LOCUS       DAOQJC010000001        92036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_85_length_92036_cov_9.064133, whole genome shotgun sequence.
ACCESSION   DAOQJC010000001 DAOQJC010000000
VERSION     DAOQJC010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 92036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 92036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92036
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85_length_92036_cov_9.064133"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1454)
                     /locus_tag="PLE39_00005"
     CDS             complement(39..1454)
                     /locus_tag="PLE39_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE66734.1"
                     /translation="MKNRITRRDFLKALSIGTASALALSCGAVTPQPEEEETSEPMPN
                     QDTSTSTSADVLVLGAGIAGLAAARTLADRGMSVIVLEARNRVGGRMWTDTSLGLPLD
                     LGASWIHGVNGNPIAKLAKQFGVATAPTDDENGIAFASDGSELPDDEFARMEALFEDI
                     YEEVAAMQEDTDEDMPLQEAFDEVIASRDLSEADLRRLNYYAYLGTALEYGADMKDLS
                     LWEWDQDEEFDGEEVIFPGGYNQITDGLAKGLDIRLGMVVNTVRYGADGVEVETSGGS
                     FVADKAVVTFPLGVLKQASVKFDPPLPESKQTAIGRLAMGVLNKVYLKFPEAFWDESI
                     ETINYMGETLGEWCDWLSFAPFTGEAVLMAFHGGDKGFDLEDLSDDEIREGAMKTLRV
                     MFGDDIPEPEGMLVTRWGKDPFAFGAYSHIPPFASGDDYDALFEPVEDVLFFAGEATW
                     REHPSTVHGAYLSGVAAAGEI"
     gene            complement(1489..1857)
                     /locus_tag="PLE39_00010"
     CDS             complement(1489..1857)
                     /locus_tag="PLE39_00010"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNE66735.1"
                     /translation="MKKLLPIVPRWLPALLMMLAIFAFSSRTSKELPNYGGWDYFIKK
                     GAHAVGYGLLALAYLDALPRRNYALAWLLALLYSATDEFHQSFVPGRHAAWTDVLVFD
                     NLGAMIALLVHYRKSRNVDL"
     gene            1870..3576
                     /gene="pabB"
                     /locus_tag="PLE39_00015"
     CDS             1870..3576
                     /gene="pabB"
                     /locus_tag="PLE39_00015"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNE66736.1"
                     /translation="MIKKDEIILRNDREWLHFSKPHRVISTENIHNAGETLLEIERLV
                     NENHWYAAGFVSYEAAPAFDSALRVIESDEFPLLWFGLYDPPQSVQLPTTVKDFSNLH
                     WEPTTERSVYNAAITKVKEQIASGKTYQVNYTMRLQTEFTEDPWEFFLFMAQTQNKYA
                     AYIDTGRHVICSASPELFFELDGEMLTGRPMKGTVKRGRTTKEDGELANWLHHSVKNR
                     AENVMIVDMIRNDFGHIAEIGSVHVPQLFTVETYPTLLQMTSTVKAKTKAPLAEIFNA
                     LFPCASITGAPKVSTMNIIAELETTPRKIYTGSIGTIAPNRKARFNVAIRTALIDRKN
                     NKAEYGVGGGIVWDSTSTDEYSEALLKARVLNQRPQGFSLLETLLWQPESGYFLFEKH
                     LERMADSAAYFGFEFSPETLHTHLAALAENFTSPKRVRVLADRFGSLTSEAKDFHPQE
                     KPFKITLAQKPIDSQNVFLFHKTTKRDMYPAPVYDDILLFNEKEELTEFTIGNLVIET
                     DEGKFTPPISCGLLGGTFRAHLLETGQISERVIQKDELANCKAIFLVNSVRGWVKVEP
                     DF"
     gene            complement(3650..4102)
                     /locus_tag="PLE39_00020"
     CDS             complement(3650..4102)
                     /locus_tag="PLE39_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66737.1"
                     /translation="MKKYSILLAIVALVLASLACQTIMGGGDNGFEAPDVPQQTDGDD
                     VEVPSVEQDGGDITVGGESPFPVTSDAFNVISTPQSVTYQTNMSGDDVMKFYRDELGK
                     QGYTEDTSMTTNFSGMIAMFFVNNGKTIVIGGAPVGDGSTSVTVAYQQ"
     gene            4186..6231
                     /locus_tag="PLE39_00025"
     CDS             4186..6231
                     /locus_tag="PLE39_00025"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNE66738.1"
                     /translation="MNRLSQATSPYLLQHAHNPVDWYPWGSEAIEKAKAEDKPIFLSI
                     GYAACHWCHVLAHESFEDPETAAFMNEHFINIKVDREERPDLDGIYMQAVVAMTGSGG
                     WPMSVFLSHDLKPFYAGTYFPPVRRFNLPAFMDVLTGLANAWKNDRAEIEKVGEQVNG
                     HLQYQNQSKNTEAITQEHLEPIAKALQGAYYWGYGGWGDAPKFPQPMALEFLLHHSIA
                     NKKDEYLKLIDHCLRIMARGGMYDVVGGGFSRYSTDNFWRVPHFEKMLYDNALLARAY
                     LHAWQVTKEPFFKQIVEETLGFVARELTHEQGGFYSSLDADSEGEEGRFYVWTLDEIR
                     AVLKDDSEFFEAAYGISPQGNWEGKTVLQRALDDTTLAARFKLDPETVPAKLAESHSR
                     LLSARASRIRPGTDDKVLTAWNGLMLAAFAESARVLAGSSPHSHTHLDLATRSAEFLV
                     NNLRVNGKLHRSWRNGQVTEEVFLEDYAALILGLLELYQTDFNNKWFTIAHELTDEMI
                     EKFSNPDGGFFDTPHTGETLLFRPKDISDNATPSGNALACEALLKMAAYTDQGSYRSL
                     AEQSLALIGESILRYPLGFARWLSAAENALGTMKQIAVLGEAGEENFERLLKVIRAEY
                     RPGVVVAASSHPPSQHAPALLENRPLLNGKATVYVCEGFVCKQPTNEIEVLIAQLKA"
     gene            6282..6920
                     /locus_tag="PLE39_00030"
     CDS             6282..6920
                     /locus_tag="PLE39_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNE66739.1"
                     /translation="MEMFYENLIKILLAVVVGGVIGAEREYQDKAAGFRTIILITLGS
                     ALFTIFSVTMDPGFTRSRVAANIVTGIGFLGAGAIVREGGKIGGLTTAATIWLSAALG
                     MGIGAGDVYISLTATVITLLVLLVFPRIEARIDRVREARSYRIVIGASGFQKIDKVEK
                     TLKECSLRVLEHHQSKSESEIISVWKTIGSPSNQEKFVQLMLKDKDIRELDY"
     gene            complement(6994..7182)
                     /gene="tatA"
                     /locus_tag="PLE39_00035"
     CDS             complement(6994..7182)
                     /gene="tatA"
                     /locus_tag="PLE39_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNE66740.1"
                     /translation="MLGLPRGAEWIIILIIIVLLFGPGRIGKVAGELGRSIKSFREGL
                     NPGEEQAADTAEKKEENK"
     gene            complement(7240..8343)
                     /locus_tag="PLE39_00040"
     CDS             complement(7240..8343)
                     /locus_tag="PLE39_00040"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNE66741.1"
                     /translation="MPFAHVTGWGMYVPEPVLTNDDISKMVDTNDQWIRERTGIRERR
                     IAREHEFPSTMGVEAAVKALQVANLAPTELDLIICTSSSPEYIFPATACIIQDQIGAT
                     KAGAFDLQAACTGFIFAVNMAAQAIRSGSIKNALVVGTEVLSRFVNWKDRGTCILFGD
                     GAGAFVLQASDVPGGVLSAVMHSDGSGADTLTLPGGGSHFPASELTIHEGKHFVHMDG
                     KEVFRFATRVMGRAVMESLEQLKMTTDDIHCIIPHQANYRIIETAAKYLKMPLDKFVI
                     NVDRYGNTSTASIPIATVEAIQNGRLKSGDKAVFVGFGGGLTWGAMVIQWTGPIPAKK
                     NVHPEQYRYFARLRSLLRRVWRYIEGFFSRKSL"
     gene            complement(8354..9595)
                     /gene="fabF"
                     /locus_tag="PLE39_00045"
     CDS             complement(8354..9595)
                     /gene="fabF"
                     /locus_tag="PLE39_00045"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNE66742.1"
                     /translation="MRKRVVVTGLGCVSPVGNTTKDTWQALLEGKSGAAPITTFDASA
                     HKTKFAAEVKGFDAAALFGTRDARKMDRFTQFATAATLEAMAQSGLKVDESNRDRIGI
                     LIGTGIGGISSLLEQYDIMRERGPERVSPFLIPMMISDGAAGNIAIRLGVRGPNMSLA
                     TACASGTNALGEAAEMIRRGAADVMIAGASEAAINAIAMAGMNSMTALSTRNDEPQRA
                     SRPFDKDRDGFMMGEGAGVLILESLEFAQARGANILCEFSGYGTTDDAHHISAPAEDG
                     AGAVGSMSLAMEDAGLKLEEIDYINAHGTSTYLNDKSETSAIKTVFGERAYKIPVSST
                     KSMTGHLLGASGAVEAVFCAMAIMENILPPTMNYETPDPVCDLDYVPNQPRKAEPNHV
                     MSNSFGFGGHNATLILSRFNK"
     gene            complement(9717..10910)
                     /locus_tag="PLE39_00050"
     CDS             complement(9717..10910)
                     /locus_tag="PLE39_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE66743.1"
                     /translation="MTKPILLLDLDDTLLNTNMEAFVPAYFQALAKELSTYVTPTAML
                     PALMAGTNAMNVSDDFSRTLEQVFNAEFYPRMDASPETLRPPLENFYDHVFPLLGNVT
                     SQRPEALEFVQWALSKGYRIAIATDPLFPLKATHSRLRWAGFEPEQFEIVSSFEHFHF
                     SKTHPAYYAEILGRMGWPEVPVLMVGNDIERDIKPSKMLGLAAFFVENEPASQSGPEA
                     GLRGNLVDLRRWLDSTDLSALIPSFKSKDAVLGILSATPAAMNSLLSGLDETTWPREH
                     SDEDWAMIEIICHMRDTEREIHQMQIRLFAEQSEPFIPRPDTGVWASQRDYLHENGPQ
                     ALKEFNDARRETLALLSEVSAEAWGRRARHAIFGPTDFLEVVSFMADHDRMHLQQIWT
                     TLKKL"
     gene            complement(10941..11270)
                     /locus_tag="PLE39_00055"
     CDS             complement(10941..11270)
                     /locus_tag="PLE39_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66744.1"
                     /translation="MKTPAGRECPHFYGNYFRGRNDEECRLLKLHGQAWTRDLCKTCP
                     VPEIARANSCQHMQLKVTVERPILAMFQRRVQVSAFCEKSNQNVSEPQIGCGKCHDLP
                     FKFEIKE"
     gene            complement(11387..11944)
                     /gene="efp"
                     /locus_tag="PLE39_00060"
     CDS             complement(11387..11944)
                     /gene="efp"
                     /locus_tag="PLE39_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNE66745.1"
                     /translation="MIDVNELRKGVTFELDGNLLKVLDYSHNKTGRGNANIKVKARNL
                     LTGANIERTFNSGQSVQDVSLDFANVSYLYNDGDLYYFMDNATFEQPGIKKESLGDSA
                     KFLTEGMEVKLTFYKGEAIDVELPTSVDLKVVEAEMAIRGDTATGVTKKVITETGVVV
                     QCPNFVVVGDVIRVDTRTGDYVTRV"
     gene            complement(12057..12551)
                     /locus_tag="PLE39_00065"
     CDS             complement(12057..12551)
                     /locus_tag="PLE39_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNE66746.1"
                     /translation="MAIRIGIMTLSDRSARGEREDASGPALADLVHAQGWSVVEQAIF
                     PDDEPAIRTALVTWADSGRFDIILTTGGTGFAPRDVTPEATRAIIQREAPGLAEAMRA
                     DSLKKTAHAMLSRAVAGIRGRTLIVNLPGSPKGAVENLQAILSVLPHAVQLLSNDPAS
                     EASH"
     gene            complement(12573..12938)
                     /locus_tag="PLE39_00070"
     CDS             complement(12573..12938)
                     /locus_tag="PLE39_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE66747.1"
                     /translation="MAKVKVLIAEDDITMVSLLKTLLKMEGFEVLALQPDADVPLAVR
                     NEKPDIVLLDVHLFGQSGLDILSDIREATETATTRVVMSSGSDVREECARRGANGFLM
                     KPYMPDDLITILKQVLQAA"
     gene            complement(12904..15483)
                     /gene="lon"
                     /locus_tag="PLE39_00075"
     CDS             complement(12904..15483)
                     /gene="lon"
                     /locus_tag="PLE39_00075"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNE66748.1"
                     /translation="MGETDEEMIKMFMTSALARFQKDDGASHAPQPDDKNEGQKFPDT
                     LPILPLRGVVVYPNTAVPLTVGQPRSIKLVDDVVGGDKLVGLVAALNPELETPGPNEL
                     YQIGTIATVHRLLRAPDGTIRLLVQGMERFRVEEFVADEPYLKARISLIPETVESNLE
                     TNALARNARDQFQQITQMIPSFPDELVGSITSLEDPLQTVYTIANFQRINLQDAQEIL
                     ELDSVYEKLKKLIGLLVREAEVLQIGQKIQNEARGEIEKVQREYFLREQMKAIQRELG
                     DRDEQSVEADEFRKKIEEANMPEEAHKQAKQELDRLSRLPTAAAEYGVIRTYLDWLVS
                     LPWSKSTQDNLEIAHAREILNKDHFGLEDVKDRILEFLAIRKLRIDRKDDEKAPSDDL
                     IRREREGVILCFVGPPGVGKTSLGQSIARAMERKFIRASLGGVRDEAEIRGHRRTYIG
                     AMPGRILQSLRRVDSKNPVFMLDEVDKLTYDFHGDPASALLEVLDPEQNSEFRDNYLE
                     VSFDLSQVFFITTANTLETIPGPLRDRMEIIFLSGYTENEKMSIARGYLIPRQIRENS
                     LRAEEISFTDDALRMIVRQYTREAGVRNLERKIGAVCRKVGTKIAEHKGEKFEITPEL
                     VEEYLDHPIFLGTEELNKRTSIPGVVPGLAWTSFGGDILFIETTAMPGGRGFQVTGSI
                     GNVMQESARAALSYVRSRAASLNVPEEFFNKYDLHMHIPAGAQPKDGPSAGVAMATSL
                     VSLISGRKVKPQIGMTGEITLRGQVLAIGGVKEKVLAAHRNGLTTVILPKRNETDIDD
                     VPDEIRQSMKFIFAESVEDVINAALEKNKPEEKKAGKPRRKKSQPTKVKKNGKSKSTN
                     RRR"
     gene            complement(15536..15937)
                     /locus_tag="PLE39_00080"
     CDS             complement(15536..15937)
                     /locus_tag="PLE39_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNE66749.1"
                     /translation="MPAIVRKSSAALLETRREILHAVSWHVRSNVWSPPTDQYETEDT
                     YVVRVEIAGMNEDDFEVSVENNNTLLIAGVRTDAQERRAYHQMEIRFGKFATALGLPG
                     PVDIEHALAEYKDGFLTVTLPKATPKQIKVE"
     gene            complement(15977..16052)
                     /locus_tag="PLE39_00085"
     tRNA            complement(15977..16052)
                     /locus_tag="PLE39_00085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16017..16019),aa:Arg,seq:tct)
     gene            complement(16062..16505)
                     /locus_tag="PLE39_00090"
     CDS             complement(16062..16505)
                     /locus_tag="PLE39_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66750.1"
                     /translation="MSLLKPNAKTPFHIDFEWWKKNERDWHVFLRSLLCPEHQEAFAE
                     MDEGGMIDWIDPQTAEVRQVAGVQHALMSHCALLPEFSDARTAMVEAVFRTFLVNGNT
                     PMSAEELSQKLGRPADTILKTIAGPRVYRGLRPFEVGLPIAESGE"
     gene            complement(16502..18073)
                     /locus_tag="PLE39_00095"
     CDS             complement(16502..18073)
                     /locus_tag="PLE39_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66751.1"
                     /translation="MAGAFSLPNEYWLSIQITPQDVENLHNYLFERETPLTTRDLSVE
                     LVETRIKAERAAAEGKKKAAGRSYLPKEKYQVGEELIFPALDWKRGTVTALREGVNPS
                     VTGFDVLTVTMEDRVERMFAANLEAHYLNDAPIVEPETNEMSAEMVLREFGEQIEKKL
                     EASFTADSGLVKIAGSWFPRALLIDINQGQLNLAEAVLDMAGGEPLTTEALMKDIELP
                     ASVNPKLAEFSLNYALQSDERFDEVGPAGQVLWCLYRLEPDLVREVPWPLRYSEIEHD
                     RSVLGDEIKRLEDQLDDELTPVDEDDARGDISSITITLIYPHLRAGTLPMSARARRLF
                     PTAYESSRVRFTLVDGKTKQSIPAWVVLKHGYVFGLREWYKAHQLIPGSIVQLKRGEK
                     PGEVIVEVKSQRSSKDWVRTVLVGKDGGFVFAMLKQPITAEFNDRMVIHVPDFKSLDP
                     VWEKKQSFNELVLSVMRELTKSNPQGHVHAQELYAAINLVRRVPPAPLFALLESNPAF
                     KHVGDLHFRLDEEAE"
     gene            18255..18740
                     /locus_tag="PLE39_00100"
     CDS             18255..18740
                     /locus_tag="PLE39_00100"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66752.1"
                     /translation="MNKALIFSLTLFTILIAACGTQATQTPPPPATVTEAPTIPAATD
                     TAIPAPTEAPILQAETPAQTQETAATVSFAKDIMPIFEASCTKCHGVEQVKEGLDLRT
                     YETLMAGSFNGSVITPGNANDSFLVEQIVEGEMPKRGQKLLPAQIQLIIDWINAGALN
                     N"
     gene            18899..19576
                     /locus_tag="PLE39_00105"
     CDS             18899..19576
                     /locus_tag="PLE39_00105"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNE66753.1"
                     /translation="MVLRDRISPELSEFLRYLASHEEATGGLPSLNDLSRELGISLAA
                     LREQLEVARALGLVEVRPRTGTRRLPYSFAPSVMKSLAYALTLDQSNFEKYSELRNHI
                     ETAYWDEAVSLLTEEDKLELKNITARAFEKLHAKQVQVPHEEHRKLHMLIYSRLNNPF
                     VTGLLEAYWNAYEAVGLNMYAGGIDYLEEVWGYHKTMVQSICNGNYAAGRKALITHID
                     LLAHRPT"
     gene            19590..21527
                     /locus_tag="PLE39_00110"
     CDS             19590..21527
                     /locus_tag="PLE39_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNE66754.1"
                     /translation="MNKIINDFSITVGTVNGSGSSTANNTILRSIFKMGIPVSGKNLF
                     PSNIQGLPTWYTIRANKDGFIAHNERRDIVIAMNPNSFVRDLASVTPGGAFFYADDIK
                     QPINRTDIAIYPMPVKTIVKNDPNVPTDFRDLVGNMVYVGVLAQMINLDMDKILAALT
                     FHFKGKQKPIDMNYGAVKAGAEWARSNLEKKDPFYVETMNKTEGMIMADGNTAAAIGS
                     IFGGVQFAAWYPITPATSLAESLNDYLPQLRKREDGKHTYAVVQAEDELAALGMTVGA
                     GWAGLRSMTSTSGPGLSLMTEFAGLAYYAETPVVIWDVQRIGPSTGLPTRTSQGDLTF
                     THFIGHGDSHSVTLLPGDVFECFEFGWRAFDVAERIQTPVFVLSDLDMGMNQWMSKPF
                     EYPSTPMDRGKVLFEKDIEELKGNWGRYLDKDGDGIGFRTFPGNKHPLSSYFTRGTGH
                     DEYAKYSEEADIYLKNMDRLKRKHETAKKYVPAPVMHSIKGATVGIIAYGSTEAAVVE
                     AQHQLEKDHGIKTDFMRVRAIPFTKEVDAFLAKYDQIFIVEMNRDGQMYQILLTEYPQ
                     FANRFKSVAFGDGMPASAKWVREGILAKYAKVGTPKKAAAAKTASKAKAVSGSKKAAT
                     KKPAAKKPTAKSKSSVKSKRK"
     gene            21552..22610
                     /locus_tag="PLE39_00115"
     CDS             21552..22610
                     /locus_tag="PLE39_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNE66755.1"
                     /translation="MTEALPMAGTPATNVNLAGLSKNEYRGNPSTLCQGCGHNSISNQ
                     IVTALYEMNIVPEDVVKFSGIGCSSKSPTYFLNRSFGFNSLHGRMPSIATGALFGDHH
                     LKGIGVSGDGDSASIGMGQFKHVMRRNVNMLYIVENNGVYGLTKGQFSATAEKGLSLK
                     KQGENPYMPVDICMEALVSNATFIARSFAGNPKQVKELLKAALAHDGIAVLDIISPCV
                     TFNNQENAYHSYAWGKDHEEPLHEISYIAPRNEIILEREMEDGEVREVAMHDGSTVIL
                     KNLEKGYNPTNRFEALRVMEEAGRNNWLITGLLYVDTSKPNLTSMYNLVDTPLNRLTE
                     AELRPDRSMIDKVNALMF"
     gene            22688..24013
                     /locus_tag="PLE39_00120"
     CDS             22688..24013
                     /locus_tag="PLE39_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE66756.1"
                     /translation="MTTETTNEKLDFKRILPVLVIVLVDLMGLSIIIPLLPLYAARFG
                     SSPLVIGILQATYPMMQFLGAPILGRLSDRFGRKPILVVSQIGTLIGFIMLGFANSLL
                     LLFISRIIDGLSGANISTAQAAIADSTTEKTRTQGLGLVGAAFGIGFVLGPILAFIVL
                     AATGQNYQAVAFTAAFFSLASILLTLFWFKETYAEVENPEASRKRRPFSFGALREALS
                     RPSIGFLLILMFFYQIAFGGYEQLFSLFTLTRLGMDARDTSGLFVLAGLFIIVVQGGL
                     IGRWSKQKGDRWLVLMGIGTLAIGLIGTALTPAVPIPWYEKAKVLESMAGQGEFRVSI
                     QSINIQLPDEAAKGWLGIIWLLLASFPAALGGGVLQPAVNSLITKASDKNEVGGMLGA
                     SSAAYSAANAIAPLFYGALFQWFGGPVPFFAGGLILAIMWLLAPRVVKA"
     gene            24066..24464
                     /gene="arfB"
                     /locus_tag="PLE39_00125"
     CDS             24066..24464
                     /gene="arfB"
                     /locus_tag="PLE39_00125"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNE66757.1"
                     /translation="MKNLESEISLEYIRASGPGGQNVNKVATAVQLRFDITNSPSLAS
                     DVKGRLIRLSGSRVTAEGVLIIEAKKYRTQEQNREDALQRFYDLVAKANEKPKVRHKT
                     KPTKASKEERLKEKKNRGSIKKIRRDKAYD"
     gene            24471..24866
                     /locus_tag="PLE39_00130"
     CDS             24471..24866
                     /locus_tag="PLE39_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE66758.1"
                     /translation="MSPSIAHVTYLVRDYDEAIAFFTEKLNFILLEDTPLDDNKRWVR
                     VSPQGGNGSALLLAKADTAEQTQHIGNQTGGRVAFFLHTDDFQRDHQHMQSHGVRFAE
                     DPRHEPYGIVAVFFDLYGNKWDLLEPRNL"
     gene            24904..25701
                     /locus_tag="PLE39_00135"
     CDS             24904..25701
                     /locus_tag="PLE39_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNE66759.1"
                     /translation="MDFSFIQITDHHLTDSDSEFLRGFSTRYALRTVLRHIAHNAGTH
                     VDFIVSTGDLVDIPSEASYQASLELLGVQNNLAEIPGPLHISVEGLQNLPMFVLPGNH
                     DDRKNFYKYLFPQSQSASIMNAAFIHKGIQFICLDWGPESKATAHPEMLEFLAQTIKK
                     DIPSIIMMHHQLVKLGSKWLDDFIADDTSRFWEIIAQKPVLGIFCGHVHATYESIVNE
                     VPIFGLRSTAPQFVLQDEPLACLLPPHYRLVTIQNDTLTTQIFEVPL"
     gene            25729..26127
                     /locus_tag="PLE39_00140"
     CDS             25729..26127
                     /locus_tag="PLE39_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE66760.1"
                     /translation="MADMAKDFIKAAKKADLDALKALLKADKSLLNARDTDGSTALHC
                     ATWKGHREVVAFLLKVGADVHAQNSNDHWGTTPLHAAAHANQAVLVQMLIEAGADVSA
                     KDMNGKTPLHHTTFHKATAAAKVLVQHGGK"
     gene            26133..26870
                     /locus_tag="PLE39_00145"
     CDS             26133..26870
                     /locus_tag="PLE39_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE66761.1"
                     /translation="MITALEEFSLNAWAALQTVHYDGWVLRFANGYTKRANSINPIHA
                     SSIPLDEKIRFCEEIYKSRQLPVVFKLTPHVYPEMLDEELSARGFQKSPSSTSVQTLK
                     LDFSDFHLPLQTELEESLSSAWLNAVCHMNGVAEENRETLRQILLNVVPRHCFLSLKF
                     DDRIVACGMGVLQSEFLGLFDIVTDPAFRNRGYGRQVVEGLLAWGQQNQAKQAYLQVE
                     LHNTVALHLYSSLGFKEQYQYWYRSKP"
     gene            27095..31291
                     /locus_tag="PLE39_00150"
     CDS             27095..31291
                     /locus_tag="PLE39_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE66762.1"
                     /translation="MNKTSHFLKPPKNNTPLFHWVGMGIVFTFGVFYYLALAVFLPRY
                     GEGVTIFVVLPVAVAGWIFGKKIGVWVGVFSFPINLLLLALANRGQFDRAMSLRWAIE
                     IDFFLAVAAGFTSGYAGEIIQKKDKNQDWESFLTAMEAVPHGMLIADSNGKISLANKQ
                     AENIFGYEASGLQGIGIEQLVPEPIRHHHTFHRSDYLNHPHPRQMEGRELTAIRRDGS
                     TFPIQVGLNPIWNNGQISILVSAMDMSLQKQAEAQRQSLMEIMQAAVETNNLQEYLAK
                     VHAALKKALSAENYFVILKNKTTGMYEGAYSVDQINAALAPQSEMQKSASAYVIRTGK
                     SLLLTEERFQQLIAEGEMELIGSRSLSWLGAPLKISGESIGVMVVQDYEKEFCYSKAE
                     EEFFTSTAGQVALAVERKRAEEELRASQQHFYELFENVPVGIWESDFTSTREYLNELS
                     DAGVTDFAAYFDAHPEAVNECVSRIKILNVNQAALQFYGVEDKSKLIDKSTTPIRIDS
                     KSFRVFKEQFIALINGKVSYDTEFEALNMRGEIRTVTMWLRLAYGAEQTWKRVFVVFS
                     DITERKLAEQAIQTAEFRYRQIMEQIPAVVYSAGLGPDMRWKFVSPQIKDLTGYTAEE
                     WIADQQIWQRSLHPGDRQLVTDVETRSIITGEPVKLEYRLYTRDGRMIWVRDLATVAS
                     DENGNPFLSLGFMFDITEQKLAEEQVRRRADEFALLYDTAHSLAKSQADLRSVLQVIA
                     ARAESMLDSNSAGFYLYDATREQLDLAVRIGEMFPAGPVRLKPGQGVSGLVVQTRKTI
                     IVNEYSTWENRSPLFDGLNVKAVLGVPMVYGGEMIGVLTIGELGSSHRKYTDADARLL
                     ELFASQAAAAIYNARLLENTIRRLDELETIARVSGTLRAANSLEEMIPGLLDETLKAL
                     DTNAGSIYLYDPASGTLQSYIQRGWFGEKMPHSQRPAEGIAGFVYSSGEVYHLKESMQ
                     EWKEIPNGWDAVCIPIQTSQEVVGVLFVSVQMPRELSSEEVRLLTTLAEIAGNAIHRT
                     RLHEQTKRNLTHISILRQIDQTIASNFDLSVSINMLINNALTQLQADAVSILRLRPAT
                     QMLEYFAGHGFKNEGMIRQVRLRLGDKHAGQAALERRQVSITNLSNSDQFFSNSDFAR
                     LENIRAYHANPLIAKGEVKGVLEVFHREPFHPDKEWLELMDTLAGQAALAIDNAELFN
                     NLQRSNQDLKFAYDTTIEGWSRALDLRDRETEGHTQRVTEMTMLLASSMGLQADELVH
                     VHRGALLHDIGKMGVPDQILLKPGRLTIKEWKIMRMHPVYAYEMLQPIAYLRPALEIP
                     YAHHERWDGSGYPRALKGEEIPLAARIFSVVDVWDALLSNRPYRRAWKQQRVVEYMET
                     YSGKYFDPKVVNSFLTLMSK"
     gene            31314..31922
                     /locus_tag="PLE39_00155"
     CDS             31314..31922
                     /locus_tag="PLE39_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HNE66763.1"
                     /translation="MDVFSLLDTVQTIARNGLLHVSNEYDKERYERLLEMAAKTYSDI
                     LNIPSEKIKQGFSNELGTITPKLGTNAAIFNERGEILLMERVDGSGWCLPAGFVEPNE
                     SPADGILREVREETGLEVEIKQLVGVFTRKPSAKMGPHTVVSVVHLCEIIGGELKISH
                     EGHALQYWAMDDMKNWHASHEIHARAAYKMWASERLIPAVSE"
     gene            31975..32850
                     /locus_tag="PLE39_00160"
     CDS             31975..32850
                     /locus_tag="PLE39_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE66764.1"
                     /translation="MHKHSVAGLFAGLGAASIWGGMYVVSKVVLEVIPPFALLSIRLV
                     MGALVLGIIIYFRKDKVVFSKEQFWKSFFVGVVGYGVSLGFQFVGTKLSTASNGSLVT
                     SATPAFVLIFAPFLLGEKTTTRRIVALVVSTLGVLAVIDPRNAELSPTLFWGNMSLLA
                     AALTWALYSVLVRKVSQSGDLLTSSTVMLLGGLPSSIAFGLWEVNTQGVGAITPGIIG
                     GLLFLGIVSTAIAMFLWNYAFAELPAAVASLTFFAQPVVGTLLGWFFLAEKITPLFLF
                     GGALISVGILIATRE"
     gene            complement(32966..34453)
                     /gene="pyk"
                     /locus_tag="PLE39_00165"
     CDS             complement(32966..34453)
                     /gene="pyk"
                     /locus_tag="PLE39_00165"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNE66765.1"
                     /translation="MRRAKIVATIGPASDSEQALEALMKAGMNIARLNFSHGTHEQHQ
                     KRVAAIRAVSKRLGMPIGILQDLQGPKIRVGKLAAPLQLAVDEQVCLYATNDEPPQTE
                     NQLLPVDFRELFDSVHEGDRLLLDDGRLALQVISAEGRVVHARVLVGGTLSSHKGINL
                     PGVKLRIAGFTEKDKTDLAFGISQGVDAVAISFVRSADDVRSVRAAIKEFSQGRFVTP
                     LLIAKLEKPEALNELEEILDVVDGVMVARGDLGVELPPERVPSLQKHIIRSANARAKL
                     VITATQMLESMIQNPLPTRAEASDVANAIFDGTDAVMLSAETASGEYPSEAVSMMSRI
                     VSEAESHFTEWGSRQDGRAGLGDSDAASMARAAKALATDPEVSAVSVFTMQGRSAWLM
                     SKGRPSKNILAFTPEYETFNQLSFLWGVQPNLTKFANSMDEMLANVDAVLLNSGIRSG
                     QQVVLVCGYPVGAQRPPNMALLHTVGSDASVNLARKLAESNVVKR"
     gene            complement(34684..35994)
                     /locus_tag="PLE39_00170"
     CDS             complement(34684..35994)
                     /locus_tag="PLE39_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNE66766.1"
                     /translation="MTTASIYQSRVTTAQEAVKTIKSGNRVFLTGNVSVPQKILAALV
                     EYAPNLTDVEICQALTVGSADYVGPEMEGHLRVNSMFISANIRKAVQEGRADFTPVLL
                     SEFPLLFKRGVLPLDVAVIHVSTPDEHGFCSLGVEVGLTKSAAESAKIIIAEVNEQMP
                     RTLGDAFIHVSRLTHIVPVNYPIPEHRMTAEGDMDIVQKIAGHVASLIPDGATMQLGI
                     GSIPDAVLKFLFDKKDLGIHTELFSDGVIDLVNAGVLTNARKSLHPGKVVAGFILGTK
                     RLYEWVNDNPMVEMHPTEYVNDPFIIAQNDRMVAVNSAIEVDLTGQICADSIGPKLYS
                     GVGGQLDFVYGASRSKGGVPIIALPSVATLRDGTQLSKITAMLKTGAGVVTSRNHVRF
                     VVTEHGIADLYGKTIRQRAQALINVAHPQFREELTRQAKELHYG"
     gene            complement(35996..37087)
                     /gene="ald"
                     /locus_tag="PLE39_00175"
     CDS             complement(35996..37087)
                     /gene="ald"
                     /locus_tag="PLE39_00175"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNE66767.1"
                     /translation="MYIGIPKESRTFEYRVGLSPAGVEILTQNGHQAYVEHEAGVMAG
                     FSDADYEKAGARLVFSKEEVFGRADLLLKVSRPMQEELNWLRPESILAGFLHLASTQQ
                     DKIDILLEKKITSVAYEQIKAPDGSLPVLRPFSQIGGAMAAQIAARLLQNNWGGKGIL
                     MGGVPGVPPAEVVILGAGVVGTSAAQMFLGMGAHVTVIDQNLVALQRIWDRFPNIATK
                     MSTKRNIERSVMYADVVVGAVLSPGQRAPILVTREMVRSMKPRSVIMDVSIDEGGCVE
                     TSRPTTHEHPVYIEEGILHYCVPNMPGAVARTATHAFVNSAIPYITEIAALGTKEILS
                     NPSLEPAINTHDGKLLNLVRLSAQQQEDE"
     gene            complement(37142..38344)
                     /gene="rocD"
                     /locus_tag="PLE39_00180"
     CDS             complement(37142..38344)
                     /gene="rocD"
                     /locus_tag="PLE39_00180"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNE66768.1"
                     /translation="MNTQDFIKMEEQYGAHNYHPLDVVIEKAEGVWVYDVDGRKYLDC
                     LSAYSAVNQGHVHPEILKALLDQAKRVSLTSRAFRNDQLPLLYKELSEMTGYEMSLPM
                     NSGAEAVETAVKLARKWAYQVKKVPRHQAEIIVASGNFHGRTVTIVSFSTEPSYRDDF
                     GPFTPGFVTVTYGDADAIEKAITPNTAAVMLEPIQGEAGVIIPPAGYLKNVAEICKKN
                     NVLFVADEIQTGLARTGKLFASHHEDVRPDIVIIGKALAGGFYPVSAVLADKPILGLF
                     QPGEHGSTFGGNPLAAAVSRAALRVIREEKLAERSQQLGEYFVEQLSEISSPHIKEVR
                     GRGLLIGVELKPEAKGARRFCEALQTKGILAKETHDNVIRFAPPLVIDKETIDWALPS
                     IREVLNMK"
     gene            complement(38433..39359)
                     /gene="argF"
                     /locus_tag="PLE39_00185"
     CDS             complement(38433..39359)
                     /gene="argF"
                     /locus_tag="PLE39_00185"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNE66769.1"
                     /translation="MKDFLAISDYSSEEIQDMLDLAAKLKKQYFKKGNKPIFKGKVLG
                     MIFQKPSLRTRVSFDMAMRHCGGDALYLSPSEIGLGKRESIADVARVLSGYVQGLMAR
                     VFDHEHVLELAKWSDIPVVNGLSDYNHPCQGMADALTIQEKFGKKSRGLNVSYVGDGN
                     NVTVSLMHVSALLGWNVTVATPEGYDVNPKAIEIAQKIAKKTKSKLTFVRDPHEAVKG
                     AHVIYTDTWTSMGQEEETAKREKIFPPYQVNAKLVGEASKDVIVMHCLPAHRNHELTD
                     DVADGKHSVIFPQAHNRLHAQKAILARLFDVA"
     gene            complement(39462..40373)
                     /locus_tag="PLE39_00190"
     CDS             complement(39462..40373)
                     /locus_tag="PLE39_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE66770.1"
                     /translation="MANTRIITIDLNFQNKTQAIASYLIRHGDANILIESGPGSTLSA
                     LKAGLAEEGLSPRNITHVLLTHIHLDHAGAAGWLSQQGAQVFVHPNGAAHMLNPEKLI
                     ASATRIYGDRMETLWGEFLPVEQNQLTVPKDAEEIAIGALRFLPIHTPGHAEHHYSYL
                     FEDLCFSGDVGGVRIPGFPYLRAPMPPPELHFAKWRESISHLKSLKFARIAPTHFGIF
                     DDVNWHLNELQKNLDEAEKWLEEIMPADPPVDELREKFTVWMNAQAASLNLSEDVLRA
                     YTLANPLGMGADGLMRYWKKVRSSPMA"
     gene            complement(40346..41662)
                     /locus_tag="PLE39_00195"
     CDS             complement(40346..41662)
                     /locus_tag="PLE39_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNE66771.1"
                     /translation="MRSGRILLALLLLIGIAGVLTTGAAIYSRFIYLSLLLGVGSWVW
                     TRWSASGMGLQRFSRLQRANVGDIFEEHFEVANNSRVIAPWIEVYNQSPIPFVSGSRL
                     FTFVLGRQKRTHLARTWLTRRGGFPLGPTRISTGDPFGLFRVSKEIPASQTLVVLPMI
                     FEIKSFLFPPGLLSGGQVIRRKSPDVTPHAAGVREYVHGDAMKRIHWPTSIRRNHLMV
                     KEFEQDPQAEIWLFLDLHRDVHLEKAYQFDEISVEAMMFGKRPRFQLPPSTLEYAITI
                     TASLTHYFIERHRAVGYASAGNTFTVHPADRSERQEGKVLETLAFVEANGKLSLAALV
                     SAQSAQMPQGSSAILVTPSTRPDLLLAVDDLQRRYLRPVVILLDGETFGGRRGSTAGL
                     VRALRERRVPVCVVACDANLSETLSEFSTEFISQDIRTWQTPVLSQ"
     gene            complement(41659..42639)
                     /locus_tag="PLE39_00200"
     CDS             complement(41659..42639)
                     /locus_tag="PLE39_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNE66772.1"
                     /translation="MADIKAFAEQVIGNLEKVIIGKRQSVELIVIGLLCQGHVLIEDV
                     PGVGKTMMARGLARSLDCIFNRIQFTPDMLPSDVTGVSIYNQQKNKFEFRPGPIIGQI
                     ILADEINRATPKTQAALLEAMEEHQVTVDGVTHSLPEPFMVLATQNPIEYEGTFPLPE
                     AQLDRFLLRLRLGYPSMTDELRVLSDQQIQHPLETLKAVVKTKEILQAIDDVKHIYIS
                     QAVKNYIVELTTRTRQSTDVYLGASPRGSLALARAGRARAAMQGRDYVLPDDIQALAV
                     PVLGHRIIVSPAARLRDLSSDRIVQEVVQATPVPGGEFSPEITSTRKKTK"
     gene            42818..42890
                     /locus_tag="PLE39_00205"
     tRNA            42818..42890
                     /locus_tag="PLE39_00205"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42851..42853,aa:Arg,seq:tcg)
     gene            43027..43860
                     /locus_tag="PLE39_00210"
     CDS             43027..43860
                     /locus_tag="PLE39_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66773.1"
                     /translation="MRSFFVLDQWEILVAPETEYTSLSWEKLKSLTPPKDRFWADPFV
                     WVQNGIHYLFIEELLFSNHRGRIACLQLDKALNIVSNQVVLERPYHLSYPFLFEYSGQ
                     LYMIPETGKNNAIELYRCIQFPDQWQFEKTLINNIRAVDTTLLESDGKWWLFTNIHEE
                     GGSSWDTLHLYYSDNPLSENWTAHPKNPIVKDIHSARPAGRIFSHNRQLIRPSQNCSP
                     RYGYAVNFNEIITLNETEYAEKTRHAFTPPSKGTIQATHTYNSAAGITVIDANIRRRK
                     F"
     gene            43873..44103
                     /locus_tag="PLE39_00215"
     CDS             43873..44103
                     /locus_tag="PLE39_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66774.1"
                     /translation="MEDSQPNTQLTRMLLSRLERISADSYWAHRASGVRGALLRMLEK
                     AEAGRPVQRSEMKRLVDLGFIILENASLEKIK"
     gene            complement(44183..46486)
                     /locus_tag="PLE39_00220"
     CDS             complement(44183..46486)
                     /locus_tag="PLE39_00220"
                     /inference="COORDINATES: protein motif:HMM:NF015149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HNE66775.1"
                     /translation="MKNKMFLILGILVILLTIPLHAAAKAPEVDVQPEKYAAIEINGN
                     NAKVPFLDLGYRERTLNSPYGITNIYFNTPLSWKLIPGGEIEINYDVFLSGADLGKVV
                     DSANPYGGNLIFTFNGQIIGSTPLEGMGSHTVRLEIPDTALTSILQDGRHQLTISLEA
                     QFSCDYDVNARIVINPTSFFDLFFEESSPELNLSRLPSPFYLENSFIPDSTLVVVADN
                     PDALELQSALNVMAGFGSMIGRVYDMQLITVSQLAGFDPTLYHMIFVGTADKFPVLTD
                     VNFKIPITDGKFVNLSPASETDGIVELALSPWNPTKVIMAVSGNSGDAVMKAGQAVSS
                     GRVFVYSDPTVAFVSNVQALSDTLPIVEDFTFESLGYVTETLSGIGLKSKEYIFYVSK
                     EQVLTKDAYVDLVYYHSGLLDYGVSSFSVELNGQVISSTPFSKESEQVTTLRIKFPPG
                     TLRFGENRLDIRASMIILPSCDTSGFSDPWFTVSNQSSIHLPVTLPGINAEPLLKDLK
                     FFPELFVTHSDLGDIAFIFPKSDVASWVIAGKLAYNLGQQFNPLIANLEAAFSDEVPQ
                     QVHDDNSMIILGLASETPFLSEFNDMLPAPFDMASNLASEKQMQVVYRIPEGVSVGYL
                     ELLLSPYNAEKSILVISGNDRNGVLLAGDSLLVNELESQLAGVFAVTNGKQVATGNAS
                     SPFSIVDNVVPGAEAVSTTPVPEILAGGPPLFERPVWLIPAIVLSSITVVGVLGYVAI
                     SAIAKRRQEKRKLLAGEEQTNQEEDKK"
     gene            complement(46585..47697)
                     /locus_tag="PLE39_00225"
     CDS             complement(46585..47697)
                     /locus_tag="PLE39_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNE66776.1"
                     /translation="MEIRFYFKIIQRGWWIILVTTLVAINVSLVYSYYFTTPMYEAVA
                     RFIVSPNMQNTESRDLVNSLEALDKRSIISTYAEVLNSHQIIESTMELLRESPNDFRG
                     YSTSVTVVPDANIIRFSVKGPDPDVAALLANSIGQHAIDYVKNLYVIYDIDFLDKALV
                     SGDPYQPQPAQDAGLALMVGLVLGVGLAIFKDQITGTIGKLGERNMLDVESTAFTRAY
                     FERRMRQEVINQPSSVLTFGVIHLLGIQEIYDSLPQAYVNQILRKVTETLKYQLRGND
                     IVGRWSQLEFSILLPSTDGVSAMNRLERIRELLNQPISLEGESGIDIVLDPRIGFADR
                     QGGESINVLISQVEKALELSMESEEKVNMYKVRPFG"
     gene            47851..49053
                     /locus_tag="PLE39_00230"
     CDS             47851..49053
                     /locus_tag="PLE39_00230"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE66777.1"
                     /translation="MFSLAFILVILLVISLGLLLYIYVGYPLIIVVLSKYRRQPIVKT
                     GTLPTATIVIPAHNEEAVINQKLDNILSLDYPRELLQIIVCDDASDDRTAEIIRTYAS
                     KGIQLSEGARAGKVGALNRALGLATGQIYVITDADTILNQNSLRELLVNFSDEKVGCV
                     VAQTRMMSSNEQETSESGGLYWRYEALIRQKESDLHSTVAATGHLMALRREIMQPIPS
                     NVILDDFYLAMMTISQGYRVISEPKSVVWERPTNTMGDDINRRRRLTAGRYQMVFMSR
                     EYLPKLSSLLRFQVISHKFLRLAIPHLMILALLTNILLVALLPALSLSPLANATLIAL
                     AILQGIFYGLALLGAVLSNTPLKKSKVFKIFMLPYFLCATNFASIAGLLWYFSGQHTV
                     LWQQAKRQ"
     gene            49057..50211
                     /locus_tag="PLE39_00235"
     CDS             49057..50211
                     /locus_tag="PLE39_00235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE66778.1"
                     /translation="METCRPLIIDLAVGYGGSSSRVLGILRALADQGAALAGLDQGAV
                     VLAAQKEGLPVYTVGAKKTDPRIVSRLVRLIREKDFQVLDTQNIQSKFWGSLAAGITN
                     TSLVSTINSWYASEHGQSSGKDRLKGRMYTALELFTNRNLDLYITVSEKDRQALMKSG
                     IPSQSIELIYNAVSFDDSQISSDPDGLRKKFNLPPQSLICTAVGRLVPVKGYDLLIEA
                     VRQVAAQIPQLYCLIVGEGECMEELSNQIKDAGLQDRVLLTGYQDRNNVMPILKSSDI
                     FAMPSRYEGTPIALLEAAAMGLPIIAAAAGGIPELVTNQEHALLVEPNNPAALAEGLR
                     KLCSDLKFAQKLGDNAQQRVRTVFNMDTQIQNTFHAYQKALDRHDAKRKK"
     gene            50290..51294
                     /locus_tag="PLE39_00240"
     CDS             50290..51294
                     /locus_tag="PLE39_00240"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNE66779.1"
                     /translation="MKVFTDAAAATGLIRIGRQFWARSLTVLNYHRIDDPHRKDFDSF
                     QPNISAHPAEFDRQMEYLSRWFNVVSLQDVVTWLTGGKPLPPYAALITFDDGYLDNYT
                     NAYPVLKKYKFPAVIYLTSGHIDTNEPFYWDLAAYCFLNTKTDHVLFPDKKELFWKNP
                     IERWQIAKSWIYAMKPLPEEEKRKWVNQLPEQLGVSIPDNFFRNLMMNWDQVREMANN
                     GIDFGGHTVNHPILTRISLEQARSEIETSKAKIEKELGRPILSFAYPNGMQTDYNTDI
                     QKIVADAGYKVAFTLQNGPTTLQEVQQSPFTIRRVFISHAHSLSQYATITSRFNRLRN
                     "
     gene            complement(51291..52550)
                     /locus_tag="PLE39_00245"
     CDS             complement(51291..52550)
                     /locus_tag="PLE39_00245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE66780.1"
                     /translation="MKNKLRIFVVEPNNGGGLVHFAYQLCNALASEGADVTLVAGAKY
                     EMADQPHSFHVEKLLNLWEHFDPDSMKAEIQNPLKRAWRKIYWTLRRMARGVRLIYAW
                     ASLTQFLLREKPDIVQFSKYEYSFESYFVGYLRRRGLIVTQLCHEFELRESQDFIWSI
                     LDRISGEVYRHFSAIFFLSEEIRKRFLSLHSTIPAERTLSVPHGNSGWLLNIPAVSAD
                     VLRQRYGLQKDERVVLFFGLLAPSKGLDDLLDAFAIARKTCNAKLVIAGYPTKHISMS
                     EMLEKNRNLGIADQVIFDARYIPLNEIQPLMALASVVVYPYRSGTQSGALQTAYTFGK
                     PVIATAVGGLPEVVENGKSGFIVSPNSPAELAEKIVAVVNDPKLGDEMGQYARHLSET
                     RFSWKTVAVQIVEVYTRLLEARKRVSN"
     gene            complement(52540..53709)
                     /locus_tag="PLE39_00250"
     CDS             complement(52540..53709)
                     /locus_tag="PLE39_00250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE66781.1"
                     /translation="MAANISAKRKYNIIFVIDGLGMGGAERLMVPILKNLSREYFEPR
                     VCVLHVKDGNPIAEDLRALGVPVDLLLIPYLRDVTAIPRLRQYLRDVKADLVHTQLEM
                     ADIMGNLAAKSMRLPSVSTMHTMPSQDMKLKSKLHQTVELLALRHFCNKVISVSEEAR
                     KFYLKISNLLPQNLLTIYNGIDLSHFEHLNHQQDRVKIREEFGLPAQAKILITVAVLR
                     EFKGIQYMIRALPAVVAKDPDVYYLVVGNGTYREALEKEVEQAGMKDHVVFAGQRSDI
                     PHLLAASDVFVLPTLTEALPTVLAEAMGASLPIIASSVGGVPEMINEGENGLLLSAGD
                     VDGLISASASLMADEGRRKAMGERGRNIVNEKFNIHVQVERLRDLYLGLIKEYEK"
     gene            complement(53699..54715)
                     /locus_tag="PLE39_00255"
     CDS             complement(53699..54715)
                     /locus_tag="PLE39_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNE66782.1"
                     /translation="MAALIDDLTTLLVSNSRRSINSNLNWVMLSNGAADPNDNVIFFG
                     FEGKATVPSFVAKVPRLPSNGRILREEYDRLAEVWGLLGKDASLLVPEPLAFFDVGQQ
                     QVSVISFVRGDGLLFSSKKKLWHDPIQVLNLSKTVAGALRELHEKTAKEAADVVSIPT
                     DFLENLELFKKIYTPTAEESFAFSELEQYFARHSVHYQTLLHGDFWHGNIIRSSQHGT
                     LIFVDWQHSRWSSDVSADVYMFLMAGALSASRGSPEERASLTAKLLVNWQQEIFPAYL
                     AAYGPTERYSLLPPRLGMQVCCIEKAVRSIRDFGYNQFDDLIWRNLLTELVNIPKDGF
                     LDGS"
     gene            complement(54715..55374)
                     /locus_tag="PLE39_00260"
     CDS             complement(54715..55374)
                     /locus_tag="PLE39_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66783.1"
                     /translation="MNSQSSPSTAAHISLPSHGVDWRFLLPLREHTRMLVIHPSDADY
                     DRSFQEFGIPFETVSFDNFNTDRSPLQFESGSFDIVAAPLGFGGDNRAGRQSQLGKNL
                     LAVRSLIRAGGMFLLGFSNRWDIRRKGREFPNSSTTWRISRSLERAGFDGMRFYGTFP
                     EPLTPEYIFPLNSQSLAFVLNYRYQHKLPGYFLKLTRTPAVNMLLGFVPFYYAVASVE
                     A"
     gene            complement(55377..56168)
                     /locus_tag="PLE39_00265"
     CDS             complement(55377..56168)
                     /locus_tag="PLE39_00265"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNE66784.1"
                     /translation="MQNHRFIFVSGLHRSGTSILFKSLRDHPEVSGFHNTNSPEDEGM
                     HLQTVYKPSGYYGGAGKFGFNPEAHLTEASPLVTEENRKKLFAEWTPYWDMSKQYLLE
                     KSPPNLIRTRFLQAMFPDSYFIVMMRHPLAVSYATRAWYKKYRIYWRRLPRIFEHWLV
                     CHEIFLEDKKHLKNAMLLNYEQFVADPSTYTNKIYEFLGLSPFPNNQKVLSSVNKKYF
                     SMWEKDQKGFLSGFVARRMISRFETRLNRFGYSLLDVERADALAK"
     gene            complement(56181..57659)
                     /locus_tag="PLE39_00270"
     CDS             complement(56181..57659)
                     /locus_tag="PLE39_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HNE66785.1"
                     /translation="MTDESVKQDAGSQFSRKVAGGLLWNSLTFGFGKLVVLLTTSVLA
                     RLLTKAEFGQVAVAVVAINYLAVIKDLGLGVALIQRRGEVDNAANTVFTINTMLGFFI
                     SALIIPLAPPIADYFRDPGVVPVLRWLGLSFAINALGSVHMIWLLRDLDYRRKFIPDM
                     GNTVVKGIASIGLAFAGFGVWAMVIGQIAGALTSVILVWIILPWRPRLMIDRSIAGSL
                     IKFGSSIIGGDILGTIIDNIDYIIVGRLFGLAQLSVYTLAYRLPEMLLIGNLWVLGEI
                     AFPAFSSIQDKPEEMRNGFLGSVRLVELIATPISLGLFIAADPIIRVVFGEQWLEAIP
                     MLRVLAIYAWVYSIGFHIGGIYKAVGRPYILFNLSVFTLIILVPALLIGAQFGVIGIA
                     WGHLIAVLIRRVVSLAVATRFVNVTLKDIFSQLVPSLRAGLVMTPLAFAVLYAASGLT
                     PIVQLILVVITGAVSYLGVAWWTEKENLFRLMKAIGGSKKKA"
     gene            complement(57729..58511)
                     /locus_tag="PLE39_00275"
     CDS             complement(57729..58511)
                     /locus_tag="PLE39_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66786.1"
                     /translation="MDAKRMMSKLRSLPALVYENFLVRSYHNLFGKQINDRFVILGHA
                     RTGSNYLFDGLISSKSITGYHEIFADHNRAIGKDFEKVLSQVYRKESKDTQLVGFKLF
                     YNHLTDEEWAKFLQHEEFRIIHLTRANRLRTIVSLDIAFKTDEWTRSSPSKGNQLDKK
                     RITLDTSKLIGRLEKIQAQEELARKRFAGRPMLEVVYEELVRIPKVVFKQAEDFLGVN
                     DIDFTKIKIMKQNTEKLEQLIVNYDEVSQCLKNSQFASCMDE"
     gene            complement(58657..60147)
                     /locus_tag="PLE39_00280"
     CDS             complement(58657..60147)
                     /locus_tag="PLE39_00280"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNE66787.1"
                     /translation="MITRLKKIEKSDVVFFVLSIVLAAGSALAIGNLDSDIGLYGLVG
                     LVGIAVALTMFVMPSFGANVLIIAVFSNVSDELSNNGYPGIIKPLVVVVVATIVARFI
                     YFEKPPAARSTTTRIEAFLFLYFLVYAFSFMMAADKSRASSVIFDVAKDIVVIYCLMY
                     TLRSPEALKRAVWVVIVVTFMLCLLGVYQSITGNYSNNFFGFAMVSLDDFSGSTAARL
                     GGPINAPNMWGQTVVSVIALVAFRIIHERRMTTRLFSMLVLGVFLFEVLNTYSRGDYL
                     ALAVVVVLIFFVFEDKFSPMIAFAGVGLIILLIPFIPSSYTERFASLSILTSGDQSGI
                     YQEDSFRGRSSELLTGLSMFKASPFLGVGAGNYKYNYQKYSQLIGIEQRAEARDPHSL
                     YVQLLAENGILGFLAFVAVIVSVFSGLNKVKRSIDYLPALRESWVPWISSLQTSMVGY
                     LVAATFLHGAYIRYFWILVGLSLTAIRLTDEMVAETNQAALSKTTV"
     gene            complement(60175..61260)
                     /locus_tag="PLE39_00285"
     CDS             complement(60175..61260)
                     /locus_tag="PLE39_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66788.1"
                     /translation="MKRIPFFIILACLLFTSCTHGSESTPVSSELGGDFDNGGDVVSH
                     PNSSEYADVDCDIPYTKDSIWNIPIDWSIAKIHPDSDKMMEAFWDGSRWIGSDPTQYA
                     PNIYFVDNKTPLVPVKLRKNRFRDAFDDKEIQYGEPAASVWMPIPEGAQPAPGTDGQM
                     VVINVDTGEEWGLNKGTIDPLGSWFANGIYRYSIENSGVPPEGFGQRGAGIGNFSGIV
                     RKCEVDLGVIEHAVTLAYDFPCTPETCGANGRPAFIPPFTKTDGRGTSTYDIPEGARM
                     IIHPEITKEEIDNACSGMKGCIVWVLAMQKYGGFIVDNSNHPKTYPEGEATANWDPEI
                     WSDDMLRNIPTEWYDILDWNYPSTTIK"
     gene            complement(61468..62043)
                     /locus_tag="PLE39_00290"
     CDS             complement(61468..62043)
                     /locus_tag="PLE39_00290"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNE66789.1"
                     /translation="MDLSLVLLSAPFWAPLLLVLGIIIKVTSPGAPALFTQFRTGKNG
                     KRFSMYKFRSMVPNAEALKEKYAHLNELQWPDFKITNDPRITKIGKILRKTSLDELPQ
                     LINVLKGDMSLVGPRPTSFGAETYKLWHTERLDVTPGLTGLWQIIGRASMEFDDRLRL
                     DIAYIERRSIWMDINILFFTVFAVFKARGAH"
     gene            complement(62148..63008)
                     /locus_tag="PLE39_00295"
     CDS             complement(62148..63008)
                     /locus_tag="PLE39_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66790.1"
                     /translation="MTNLKRSVLSIAFFLFGILVLGQLDRSDTPIINFASYFYLLAIF
                     IVPVMVFVPSLHKISVVVPMVFWGAVYFGIFQLTDRGNTGSLDVEVVVLELVIIEVGV
                     WLSFQLALGIERSESLMDVLAQGTFPHRALEIDLANEQIKVEFSRSRRYHRPLSLLVL
                     HAFPQDEAVVREMLMSLQRDVLTRLSNARVGQAIGESIRQTDLLVRDQVGRYIVLCPE
                     TDLESVYLLADRLSKIVVERTGLHVNCGVASFPDEALTFEDLLHLARDRSKQPLPSKA
                     AIEMKEQVVK"
     gene            63207..63572
                     /locus_tag="PLE39_00300"
     CDS             63207..63572
                     /locus_tag="PLE39_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNE66791.1"
                     /translation="MTVSLSGKNLFLLHIKRIEQIHPGGNFSVSNDLYNKNPSQIVMY
                     STEYCPDCHRAKKFFETNGIEYLKVGLENNEEATLFVMDINDGNRSVPTIIFPDGSVL
                     VEPSWEELRIKTAMPTETL"
     gene            63664..64311
                     /locus_tag="PLE39_00305"
     CDS             63664..64311
                     /locus_tag="PLE39_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66792.1"
                     /translation="MVKRIFTLLFLVVILSSCDTWGNLPQPLPVWTPVPSQLPNIITA
                     TPIILTPGVIVLTPVTSTATNTETPSPVPTFTFTPFTPTETPVTPIQALTVTILGCNT
                     SIDILHGMGEVTNAYVTVTNTGTTDLPNTCALLRAIDEDRDHPDKKVCVPNLPIQNQV
                     TLKLTVDSAYNKDTIIQVDASSNDLLLLRVDRQSCTDISLFGGTPSDIGVVKPIQ"
     gene            complement(64321..65481)
                     /locus_tag="PLE39_00310"
     CDS             complement(64321..65481)
                     /locus_tag="PLE39_00310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE66793.1"
                     /translation="MKILRSYLGIKLFLSYFVVILVGMTVIGITTKIVTPGAFARHLL
                     AMENQIEMGQGMGQGMGQGMGQGQGQGGGMMSEFYRSFQATFTEALLIAVAAASLVAL
                     VVSFVFSRSILSPVYVMMTASQRIAEGRYDERVQLQGTDELNQLAGSFNKMAEQLEQV
                     ENMRRRLLGDVAHELRTPLTAIKGSAEALMDGILPASDETYQQIHAEAERLSRLVDDL
                     QELSRVESRAIQLDLRAVDATVLIQTIAKRLQYQYDEKRINLTLNLPADPIHVLADEG
                     RAIQVLTNLAGNALQYTPENGSVTISVKKEKDEAQFSVYDTGTGIPAEHLAHIFDRFY
                     RVDKSRSRAHGGSGIGLTIARHLVESHGGRIWVESAGKNKGSVFSFTLPLAK"
     gene            complement(65497..66180)
                     /locus_tag="PLE39_00315"
     CDS             complement(65497..66180)
                     /locus_tag="PLE39_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE66794.1"
                     /translation="MTKILIVDDEPSIVNLVQAYLKPEGYEVFTAVDGPSGLKAARAF
                     KPDLIVLDVMLPGMDGLEVLSRLRRESQAYVILLTARTEETDKIVGLSMGADDYVTKP
                     FSPRELVARVKAAIRRIQTGTGPSAEGSVLAFRHVRIDTGARQVTVDDQTIDLTASEF
                     DLLHALAENRGRVLTREQLLERVWGGSYFGELRVVDVHLGHVRQKLGLPDVISTVRGI
                     GYRFDDETA"
     gene            complement(66294..67649)
                     /locus_tag="PLE39_00320"
     CDS             complement(66294..67649)
                     /locus_tag="PLE39_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66795.1"
                     /translation="MPTQNTIPTVVISNFAEAHIDFLGRSSDPEERDDLINGEDYHGD
                     RTILWADDPKLVIVNYPIAHAEFNVKRLGYSRTSHLAPHEPSHYLCLDILREAHLLEG
                     ILQYAAPNGIVQLIPYATTKEFLQLVEVLRREHHLQVLTPESPDADHFWLRDYIDTKT
                     GFRTLASFWMTDASRFLPFGMICETLKHAAIAAHWFCSRGEACVVKTDTGESGIGTTV
                     ISPEDHLSVEGIFEKLHSQPFYSNELIVVEKYIPSANRISPSAEVKVPKLGEGEPQIT
                     YISNQLFLGFGDFCGIQIDRSIYSEPWCADLKRCTLELAHNLQNMGYVGHFDLDCIVS
                     DDNEVYLLEINARRTGGTHVHEFGKYTFGDDYVSKVSLISFEASNSGTITDAMELLEV
                     IKDFLYPMPGDEPLGMVVTITKPLFKNRFGCIVVAPTSERALQLQAQVDEHIQRYCQS
                     K"
     gene            complement(67658..68743)
                     /locus_tag="PLE39_00325"
     CDS             complement(67658..68743)
                     /locus_tag="PLE39_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66796.1"
                     /translation="MNETKKSRVFFISLRIKLLIGFTLLFTVVFAVAFYWFYQFSTDL
                     AMQRITDDLVNTIQGATKLIDGDEFQALYTEATPREDGYTDDLRYWAQAKLLWSVKQI
                     EPRAGIYTYIKGTEPNELIFVTSGGALNNPPTGAKFMEHWTTDNVGPNLSGLEELTLQ
                     NTPEGQKNNGCIYGTPGCKVEMYSDDFGSWVSAFAPIKNSKGEVVGAIGVDFKADYVR
                     EVQAAILKKVYIAFAITYISLFFLVFFISSAFTRPILTLTKAAEKIGEGDYKGGLVFL
                     SNVGETSKFPDEIETLDKVFQSMIDKVYTREQSLRKQVEELRIIIDDSKRKKQVNEIV
                     ESEFFQDLKTKAQKIRDNQARKKSDEA"
     gene            complement(68740..69462)
                     /locus_tag="PLE39_00330"
     CDS             complement(68740..69462)
                     /locus_tag="PLE39_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66797.1"
                     /translation="MFEIPELGFEIQEIVNGNTRYLDELIELYLQLFPGYGRYVAVMR
                     RRANRPNDVVPPFLEHQWLALVNGRPAAMTVFKYNYKRNCGLGLDLGVHPDYRNFSYS
                     GYTSLARLMIELRHKQLLMDAKLLGKPLPLGSLVEVESPKVVARFKEYGMLLLPVKYY
                     EPPAPEEALEIISRHELEKTGFNPMHLGIYPIDRDAYNPKDVDQISMCVKAYLMDHYG
                     LSETHWAVQESLQSISIEESAK"
     gene            complement(69462..70808)
                     /locus_tag="PLE39_00335"
     CDS             complement(69462..70808)
                     /locus_tag="PLE39_00335"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HNE66798.1"
                     /translation="MNERITNWFGTQHVKLVLALWLLLAINMMVMELSDVVATAGFVS
                     NIGSDSLPWLWLVMTLLTLVGASGYSVLVDRYSRLNLITWLLGVMGVFYLFLMFLFRA
                     QIPDTITFPLLSIITDQQYYILPLAFWALANDVFSVTEGKRVFPLIASGAVVGSLAGN
                     SLAGLSAKLLEQLSAGTEAIFVLAALLLFVSVVVLRIAFFRHHVRARQSKDEDTSLMK
                     SMQVGVEYFTKVAMLKMVAIIMFLSGMALALLEFYFLRSIETTTSGNSLEFQQFLGYF
                     KTAQTIGLLTFQWLITRRLLEKVALKNSFMVLPVFLLSASGIALGLVGVWGVAIGRFF
                     ARMIQRGWDEPTRKSLQGLIPDERRGRVALFMDSVFYNLATIVSSMWMALLLWLMHSA
                     LITELTFQYMALGTALFAALGAMLASFNFNRVYEKSLLDWRFARSKRKSVLDNIEF"
     gene            71217..71978
                     /locus_tag="PLE39_00340"
     CDS             71217..71978
                     /locus_tag="PLE39_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNE66799.1"
                     /translation="MTDEPIKSLARNKIAMLIDGDNAQAGLLSQMLVEAGRYGQVTVR
                     RIYGDWTTQSMNSWKDTLNFHAFQPIQQFRYTIGKNATDSAMIIDAMDILHSDVVDGF
                     CLVSSDSDYTRLATRIRETGVFVMGIGEKKTPKPFVNACDVFVYTENLTSEKKTSPQK
                     NRNVDKTKKAKDEKEELDPMPILSQAFEMAVQQDGWASLANMGNALYQVDPGFDPRTY
                     GHKQLSKMISKLKDRFEMRATEKDGPIIFYVRMKE"
     gene            72056..73408
                     /locus_tag="PLE39_00345"
     CDS             72056..73408
                     /locus_tag="PLE39_00345"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNE66800.1"
                     /translation="MDSDTNQFSQREKEVIESLLQGKSNKQIALALGISPNTVEYHLK
                     KIYKKLQVSSRTEAVLQLGKSVVSHTPSTPRESAVEMNSKNADNGVKSVSTRRISLKK
                     IYAVVGTLLIIISALVLFFVNRPDPSAEGTPTNTAKLPDLAITSAYVSMVDENGNCID
                     TYVLLVTAANHSAVPVHDVAVSTANHSTQLEILEAYQSKVIFFPATATDGNYTAVIDP
                     ENLILETNEDNNSLSTFVSTPTPVADCPPPLIQTLSNNGSATQAPYPLPTATPAAPFI
                     LLASPTLLFTDSSTPISERIVHYYFITPAENPPPDGSIVVISHILAPTPSDRTYGPDT
                     ATNIRASLENVLHDERNGWTSTNVAIVDVTFNNGHTNVILQGEYFGVGDVTLIAARMQ
                     ILMTVFANPSVQTATVTLNGDTIGNMGISNSLEAKPAGYIFTRSEIETFQRDHVYGTP
                     "
     gene            complement(73427..73924)
                     /locus_tag="PLE39_00350"
     CDS             complement(73427..73924)
                     /locus_tag="PLE39_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66801.1"
                     /translation="MEQTPYSLRLLSMRPLYIFLLSSVFVFVAGCSTPQTTVNNVPVS
                     EPTSNDLPPSSVAFVDARFAAEATAIQLLETLDGANWLEGPRVLYVEEMSYEQANQKI
                     GLGEGDFVTWPQGTRVWFVIASGRWQVTQFGPNPEIYEGCLFMVYSVSGANIIAMGDS
                     VCPTN"
     gene            complement(73955..75241)
                     /locus_tag="PLE39_00355"
     CDS             complement(73955..75241)
                     /locus_tag="PLE39_00355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE66802.1"
                     /translation="MLRIAMLSYHTCPLATLGGKDTGGMNVYVRDLTCELGRMGIHVD
                     VFTRSQDEHVPHVVHELGFGNRVVHIPAGPEEPKSKSDIAKFIPEFVEGIKQFAAEKN
                     IHYDVIHSHYWMSGLAAEALSDAWGGTPIVHMFHTLGEMKNRVARSEDERAGEDRLKG
                     ERQVLGRADRIVVATLAELTQLRFLYRANARKLVVIPPGVDTSHFYPIPADEAKQYIG
                     LTPDSRMVLFVGRIEPLKGVDTLIQAMSCLGVNEIHKPVHLAIIGGEPDVIPEKMSAE
                     MARLQKLCDDLCMGGMVVFLGKRGQDTLPYYYSAAEVLVMPSLYESFGMVALEAMACG
                     TPVIASEVGGLGYLVQDGETGYTIPDSDPEALCNKLSGLLGDAHLRETMGLRAAEYAL
                     DYAWSNIAAQIVDVYKGLVEKRSVGVLLTGSDAVRS"
     gene            75336..75408
                     /locus_tag="PLE39_00360"
     tRNA            75336..75408
                     /locus_tag="PLE39_00360"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:75369..75371,aa:Thr,seq:cgt)
     gene            75491..77683
                     /locus_tag="PLE39_00365"
     CDS             75491..77683
                     /locus_tag="PLE39_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNE66803.1"
                     /translation="MNKYISEILKIKTRSFNARELVLATLVTGVVGAALMAAININRA
                     EMIPLSLLLFIALVLGYFGYLNAARWVSLFTTLAIISSLVYKNNGIRDPAILGLVVVL
                     IAAGLLAGRRGTIIVGALILAEVILFGFLETHGYITNKFSQFNTFADYFSTSLCVVLV
                     TVFQWLVIARLNENIKTAELEIRERETIEKQLRDAETRYRNLVEQIPAVVYISEPGAA
                     GIWHYVSPQIEALTGFTPEEWTLNKDLWYRLIHPDDRERTVEAELSSLNSGQMPRTEY
                     RLQTRSGNYVWVQDQSIRSMENNVLQGFLVDITARKMAEDQLQKRLAELNAVRGVSET
                     LIAQTDLKELIQQTGDQIKVTFNVDNLFISLLNATTSLIHFPYYVEKGQQIQAPSLTF
                     GKGMTSSVLRTKKAFLINTNWAQVSAEYGVIYYDNSPARSSLTVPLMIGEKGIGAISV
                     HDMEKENAFSENDLRLLSTIAANLAVAIENTRLQESLKRELTIQEKLVTELEQKNAEL
                     ERFTYTASHDLKSPLITIRGYLGYLEKDARNGNFDRLQQDIGRISEAAEKMHNLLNDL
                     LELSRVGHIINEPRNISFSEVVEEGLKRVESQLKEKQIQVKIGSDLPQINGDKERLIE
                     VIQNLVENSCKFMSDQPKPIIEIGVEKRDGENIFFVKDNGPGIRQDFHERVFGLFDKL
                     DPTSSGTGIGLALVKRIVEVHGGRIWIDSYGYGSGTTFYFTLTDKSSS"
     gene            77793..78359
                     /locus_tag="PLE39_00370"
     CDS             77793..78359
                     /locus_tag="PLE39_00370"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNE66804.1"
                     /translation="MSLKHAILGFLSFAPFTGYDLKKAFDNSVQHFWPANQSQIYRTL
                     AELEENGFVEKEVIAREERLDMKVYHITATGREELHQWLAAPLPERDDREPFLIQVYF
                     GGQLNDTELLKVFQDKLKKIEEQLALYEAVFQMTQNTPSKVSDPRTVFLSMLTLEAGY
                     INTQSIAAWLRSAIARIENKNYTINMGK"
     gene            78360..79205
                     /locus_tag="PLE39_00375"
     CDS             78360..79205
                     /locus_tag="PLE39_00375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNE66805.1"
                     /translation="MNKTIRNIIIVSLFTIGGGWLGIWLNNVTGNTQPPMQSLGVLVW
                     LTTPAFSGFLLRAFGGDGWKDSGFGLNLISGWKWYLLGILVYPLASLLTFGLGALFGI
                     VSAEGLAAQGFNAYLSAAGMIFVGSLMKNFFEEFAWRGYLTPRFEAAKLHPMLNHFIV
                     SLLWMTWHLPYYYYYLDRATLNSAITTSIPVFLLTGYLVMFPTAILFGEIRLLSKSVW
                     PVFILHNIINALSMPLLINGFIEVKGALAPVFTPTNEGILVSLVFGVVGWFLMQYRLK
                     KQVTP"
     gene            complement(79226..80953)
                     /locus_tag="PLE39_00380"
     CDS             complement(79226..80953)
                     /locus_tag="PLE39_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNE66806.1"
                     /translation="MAEYKSQIWRVNTRTQELKREPIPETWQRLGGRGLIARILVDEV
                     DAKCDPLGADNKLIFTPGLLVGHMLSSTDRISVGGKSPLTGGIKEANAGGRTGYHMAF
                     MGIHALIIEDMPKEDGYWVLHLSLNGAKWERADDLAGLGVYATAPKLLEKYGDKVAIS
                     MIGPGGEMRMKSAGIQNLDKDKIPSRIAARGGLGAVMGSKGLKAIVFDHAGGQKPPIA
                     DAETFKVAQKDYTQSVMNHPQSITYRDYGTQAMPQMTQRFAALPTHNFSRGTFYDVEK
                     ISGETMREFILNRGKPSDPSHACMAGCTIKCSNVFGGEDGKIIVSPLEYETVGLMGTN
                     LDIDSLDSIGRMNWQVNDLGLDTIEVGGALGVAAEAGLMRWGSEEDGLKLIDEIRQGT
                     ELGRVLGDGAVAVGKKYGVERVPAVKGQAMSAYEPRSIKGTGVTYATTPQGADHTCGL
                     TIRAQVNHLDPTQQKDASLNAQLNMAGYDTIGACIFAGFGYAATPDGVVKRLLKSRYG
                     WEDVPDNILQALGRETIKLEREFNKRAGFTKEDDRLPKWMTEEAIPENGSVFDVSEDV
                     LDHIFDGIE"
     gene            complement(81035..82393)
                     /locus_tag="PLE39_00385"
     CDS             complement(81035..82393)
                     /locus_tag="PLE39_00385"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNE66807.1"
                     /translation="MTKSLDRSKLETLLKQEEELFHKNHPRSYEMYQRARKSLHGGVP
                     MLWMIRWAGSFPVFVREAKGAHFTDVDGNDYIDFCLGDTGAMTGHAPEATVKAITEQI
                     QKGITLMLPYEDVIWVGEELQRRFKLPYWQFALTATDANRFALRMARLITGRSKVLVF
                     NYCYHGSVDETFITLDEEGTPISRPNNMGPQIDPRETSKVIEFNDIAALETALSARDV
                     AVVLAEPAMTNIGIIHPDPGYHKALRELTRKYGTYLIIDETHTICAGPGGYTASHSLE
                     PDFLTLGKPLAGGVPAAIYGFTEEVSKAFAAKLAMDDADVGGIGGTLSGNALSIAAMK
                     ATLQHVLTDEFYAKATALQEQFTAGVQSVIKEFELPWIVKQLGNRSEYWFRPNPPKNG
                     GEAHAAVDPELDRYMHLYALNRGILMTPFHNMALISPETTQADVDHHTRVFREAVASL
                     FA"
     gene            82504..83601
                     /locus_tag="PLE39_00390"
     CDS             82504..83601
                     /locus_tag="PLE39_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE66808.1"
                     /translation="MENTATGTLLDTFNQDKRFRVPAYYTVCFGSLSSGLRFSVFTDR
                     TRALVDDIIDCLDLERYSFITIFKNWATEDFKNARPIDDYPYEYLFKSDSGQMLVHLI
                     AREGFITVDFYYDVNRPDCEKWVVETNQNLRERFSPAKQPKFKVLMQQEGMFGTEDVN
                     TNDFESINLAEYYNDDFLEIDATISDFMAKKESGIILLHGEPGTGKTTYIKHLISRFH
                     DKNFIFVQNDFVRDLLKPSFVSFLLQNKNASLIIEDAEKVVVSRDNASEDSVVSTILQ
                     LTDGLFSDFLNIKIICTFNTDIERIDKALLRKGRMVAKYNFRALSAEKTAALAKKLGH
                     DDVKGSLTLADIFEFDKRDFKDKSKKVIGFV"
     gene            complement(83661..84938)
                     /locus_tag="PLE39_00395"
     CDS             complement(83661..84938)
                     /locus_tag="PLE39_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE66809.1"
                     /translation="MNQLYQDNFSHMTPAWSRIFSFVSERAEGAYIYTDDGKKLLDFT
                     SGIGVTNTGHCHPKVVEAIREQAGLFLHAQANIVVHKPMLQLIDELRKIVPPSLDSFF
                     FANSGAEALENAVKIARVATGRPNVIVFNGSFHGRTHATMALTTSKTIYRTGFAPLPS
                     GVYVTPFPYAFALKMTEEQASQYALDRLEYLLASQTAPKETAAILIESVLGEGGYVVP
                     PTSFMKGLREICDKHGIMLIFDEVQSGFGRTGKWFALEHFDVTPDIITAAKGIASGMP
                     LSGVFSRTDIMKKVETGSIGGTYGGNAVACAAGVATIRAMREEKMLENASERGIQLMT
                     GLRKLQEEYPQIGDVRGKGLMIGTEFIVDGTQAKAKPLVKEIIHKAEEKGMLLLSCGT
                     YDNTLRWIPPLNVSSQQINDGLKIFGEALKESV"
     gene            complement(85048..86955)
                     /locus_tag="PLE39_00400"
     CDS             complement(85048..86955)
                     /locus_tag="PLE39_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNE66810.1"
                     /translation="MPNGYNGKILHVDLTKGSLSVEEPNEAFYRKYLGGSAMGMHYIL
                     RDMPKGADPLGPDNVLTLFTGVATGAAISGQSRLNANAKSPISGGIGDSQSGGFFPAE
                     LKFAGFDGIVVKGKSSKPVYLAIIDGNYELRDAAHLMGKLTGEVDDIIHKEVDPKAEI
                     LQHGIGAENGVLFSSLCSMSNRHNGRTGMGLVMASKNLKAVVVRGTKKVQLANQKALT
                     ELNRIGPKAIPENGDMDGLAKFGTAVVVLFNNTIGTLPTRNYNEGQFEGCEPISGEKM
                     AETVLKERDTCYACVVKCKRVVEIKDGAYKVDPRYGGPEYETLGTFGSYCGIDDLAAV
                     SLANQICNEYAVDSIAAGATIAFAMECYEKGVITKEQTAGLDLKFGNADAMIKALYML
                     VKGEGEFGKTLGMGSERAAKKWGNGAEDNLITVKGAEAPAHMPQAKRSLALIYAVNPF
                     GADHQSSEHDPYYEEGVGDFNLDRLKQIGLGSPQPAYSLTEEKVRFAYETEVFYSMLD
                     SAELCQFVYGPTWTLYDGKDTANMIGSVTGWDITVEELMEVGRRRLDLFRVFNAREGL
                     TRNADKLPKKFFKQLQGTGPTAGFALTHEEVDSAIDTYYKLAGWTNDGVPTRETLKKH
                     DVEWAAEYLPA"
     gene            complement(87205..88800)
                     /locus_tag="PLE39_00405"
     CDS             complement(87205..88800)
                     /locus_tag="PLE39_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNE66811.1"
                     /translation="MKNNSGEGNVISAETKKLKRELTLLPLFGLIYFTVCGGAFGAEP
                     MVGWSGPGLALLLFILTPLFFSIPNMLMVREMQSMMPVEGGYYHWLKVAFGPFVGYMG
                     GWMNWVVSWVDVSIYPVLAAYYLGYFIPALREGAVIGGVFVDSTWLSFGVAVVLIWAV
                     SALNIRGARFSGLLTNWLGVVMLIPLIALTVIGFMAWLKSGETIHLPFLPGGAEVNFS
                     NTIGALSTGIFVAMWNFMGWELPAAAGDEIVNPKKTYPLAMVLVLVAAIATYSLPTLA
                     GLYGGAGEDGRYQMWGIEEYESGEGIGPVLDEYGVPAEQAQSWGVDTASSVGWEFPDI
                     AHVVADKLTGQTNGTLALVMGGLVTFSAVLSMIGLFIGNGLGGTRVPFAMAADGMMPK
                     FMVKVHPRYGTPWVAILFCGLIYSIFSLSAFAALVVIDVFLNMLVLMAEFFAMWKLRF
                     TMPHLERKEVPGGYFGLTLVTIAPAMIIGLAIYSQYVEEGMASISWALGAMAVGAILY
                     FPIRKWIKPGVPDVNPFVAGEED"
     gene            complement(88879..90087)
                     /locus_tag="PLE39_00410"
     CDS             complement(88879..90087)
                     /locus_tag="PLE39_00410"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNE66812.1"
                     /translation="MKVLLVGVGGVGEAIAAIAKPRAWMEQMVLADYNVKRAKEVQKK
                     LGEKKRFPVEFIDASKKSEIVKLAKKYKVDLIMNAVDPIFNEEIFDAAYSAGVMYMDM
                     AMTLSKPHPKDPFNKCGVKLGDYQFAKAKDWKKKKQLALVGIGVEPGMADVFARYAAE
                     HLFDEIEELGIRDGANIVIEGYEFAPNFSIWTTIEECLNPPVIWEEGKGWFTTAPFSE
                     PEVFDFPEIGPVEVVNVEHEEVLLMPRWVKTKRATFKYGLGDQFIGVLKTLHMLGLDN
                     KEKINVKGVQVAPRDVVAACLPDPAHLGDKMSGKTCAGTWVKGTKDGKPRQVYLYQVA
                     DNKACMKAWGCQAVVAQTAFSAVIAMDLLEHGIWDGVGVLGPEAFPPDPFMEKMADYG
                     FPYGIKEMAA"
     gene            complement(90406..90876)
                     /locus_tag="PLE39_00415"
     CDS             complement(90406..90876)
                     /locus_tag="PLE39_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNE66813.1"
                     /translation="MESLIGSGNPSSRLDNIDQYIIDAMRKDGREAFAQIAEKLNVSP
                     GMIRQRYNRLVELGYLKVVAVTNPLMMGIRTMALIGIHTDGHKMLQVAEEISKIDEVV
                     YLVVVSGRYDIVCEVFCRDHEDLLKFLTEKLAQVDGIRETESFIHLKIMKEIYF"
     gene            complement(91140..>92036)
                     /locus_tag="PLE39_00420"
     CDS             complement(91140..>92036)
                     /locus_tag="PLE39_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNE66814.1"
                     /translation="RDEYSSNEEMYAKISAGGSSYDLVQPTDYIIGLMIRQQLLQELD
                     HSKLPNMANMDSGWMDQSFDPGNKYTLPYLGGTDAIVVNTATVKTVPTSWADLWNPEY
                     AGRMIFLDDSRAVIGLTLLTLGYDVNTTDPAQLEEAKAKLAELVPNIKVFDSDSPKTA
                     LIAGDVDLGMTWTGEAFIANQENPDIQYVYPSEGPILWQDNWAIPVGASHTDAIYAWL
                     NYTMQGDVFWLTIRDFQYTNPNKAALEYAKANQPDVYKAYADSPITNPPAEAVANGHG
                     IEDVGDATPLFDDIWVEVKGGQ"
BASE COUNT        24307 a        23985 c        21776 g        21968 t
ORIGIN      
        1 attcaggaag aggtgtattt ttttaattgc ttctctgact aaatttcccc ggcggctgcc
       61 acaccggaaa gataagctcc atgcactgtg ctggggtgtt cacgccaggt ggcctcaccc
      121 gcaaagaaca aaacatcttc cacaggttcg aaaagcgcat cgtaatcgtc gccgctcgca
      181 aagggcggaa tgtgcgaata tgcgccgaaa gcaaacgggt ctttacccca acgcgtcaca
      241 agcattcctt caggctcggg aatatcgtca ccgaacatca cccgtagcgt cttcattgca
      301 ccttcgcgga tttcgtcatc tgaaaggtct tcgaggtcaa aacctttatc gccgccatgg
      361 aacgccatca acaccgcctc gcccgtgaaa ggcgcaaaac tcaaccaatc acaccactca
      421 ccgagcgttt cgcccatgta attgatggtc tcaatacttt catcccaaaa cgcttcaggg
      481 aatttcaaat aaaccttatt gaggactccc attgccaagc gcccaattgc agtttgtttc
      541 gactcgggaa gcggcggatc aaacttaacc gaagcctgct tcaacacccc aagcggaaac
      601 gtcacgacag ccttatctgc cacaaagctc ccgcccgaag tttccacctc caccccatca
      661 gccccatagc ggacggtgtt caccaccatc cccaaacgaa tatccaaacc ttttgcaagc
      721 ccgtctgtga tctggttata cccgccaggg aagatgactt cctccccgtc aaattcttca
      781 tcctgatccc attcccaaag cgacaggtct ttcatgtctg cgccgtattc caatgccgtg
      841 ccaaggtagg cataataatt cagccgccgc aaatcagctt cagataaatc cctgctggca
      901 atgacctcgt caaaagcctc ctgtaaaggc atatcctcat ctgtatcttc ctgcatcgct
      961 gcgacctctt cgtaaatatc ctcgaacaac gcttccatgc gcgcgaattc atcgtcgggc
     1021 aattcactcc catcagaagc aaaagcaatg ccattttcgt cgtctgttgg tgcggtcgcc
     1081 acgccgaatt gcttcgccag tttcgcaatg ggattcccgt tcacaccatg gatccacgaa
     1141 gcgccaagat cgagcggcaa accaagcgat gtgtccgtcc acatgcgccc gcctacgcga
     1201 ttccgcgcct caaggacaat gacactcatt cccctatcgg ccaatgtacg ggcagcggca
     1261 aggcctgcaa tccccgcccc caacaccagc acatcggcgc tggtggaagt tgaagtatcc
     1321 tggtttggca tgggttctga tgtctcctct tcttctggct gcggagtgac agctccacag
     1381 gaaagggcca gcgcagatgc tgtcccaatg gataaggctt tcaaaaaatc tctgcgggtg
     1441 atcctgtttt tcatgatttc tccaattgta atttctgtag cgttcgaatc ataaatccac
     1501 attgcgcgac ttgcgataat gaacaagtaa cgcgatcatt gcgccaaggt tatcgaacac
     1561 cagcacatct gtccacgcgg catggcgccc gggcacaaag gattggtgga attcgtcggt
     1621 ggctgaatat aacagggcga gtaaccaggc caaagcgtag ttacgacgcg gaagcgcatc
     1681 cagataggca agcgccagca gaccgtatcc taccgcatgg gcgccttttt tgatgaagta
     1741 atcccatccg ccatagttgg gcagttcttt gctggtgcgg gaagagaagg caaagatggc
     1801 cagcatcatc aggagcgcgg gaagccatcg gggtacaatt ggcagcagtt ttttcatgtt
     1861 gaggcaaaat tgatcaaaaa agatgaaatc attttgagga atgacagaga gtggctgcat
     1921 ttttcaaaac cacatcgtgt gataagcaca gaaaatattc acaacgcagg cgagacttta
     1981 ctggagatcg agagacttgt caacgagaat cactggtacg cggctgggtt tgtcagttac
     2041 gaagccgccc ctgcgtttga cagtgcactg cgcgtcatcg aatcagatga gtttcctttg
     2101 ctctggttcg ggttgtatga cccgcctcag tctgtgcaac tgccaaccac tgtaaaagat
     2161 ttctcaaacc tgcattggga acccaccaca gaacgcagtg tatataatgc cgccatcaca
     2221 aaagtaaaag aacaaatcgc atcgggcaag acctatcaag tcaattacac catgcgtctg
     2281 caaaccgaat ttaccgaaga cccatgggaa ttctttcttt tcatggccca gacccagaat
     2341 aaatacgccg cctatattga tacaggcagg catgtgattt gttcagcttc tccagaattg
     2401 ttctttgaac tggacggtga aatgctcaca ggccgcccca tgaaaggaac ggtcaaacgc
     2461 ggacgcacca ccaaggaaga tggcgagctt gcaaactggc tgcaccattc tgtgaagaac
     2521 cgtgctgaaa atgtgatgat cgtggatatg atccgcaatg attttgggca catcgctgaa
     2581 atcggcagtg tgcatgtccc tcagttgttc actgtggaaa cctatcccac acttctgcaa
     2641 atgacctcca ccgtcaaggc aaaaacaaaa gcgccgctgg ctgaaatttt caatgcgctt
     2701 tttccgtgtg catccattac tggcgcgccc aaagtcagca ccatgaacat catcgcagag
     2761 ttggaaacca caccgcgaaa aatttacacg ggcagcattg gcaccattgc cccgaaccgc
     2821 aaagcccgct tcaatgtagc aatccgtaca gcattgatag acagaaaaaa caacaaggca
     2881 gaatatggcg taggtggagg aatcgtctgg gattcgacca gcaccgatga atatagcgag
     2941 gcactactca aagcccgtgt attaaaccag cgtccgcaag gcttttccct gcttgaaacc
     3001 ctgctttggc agccagaaag cggttatttt ttgttcgaaa aacatcttga gcgcatggcc
     3061 gattcggccg cttacttcgg ttttgagttt tccccggaga cactgcacac ccaccttgca
     3121 gcactggcag agaatttcac ttctccaaaa cgtgtgcggg ttttagcaga ccgattcggc
     3181 agccttacct cagaagcaaa ggacttccat ccgcaagaaa agccgttcaa aatcaccctg
     3241 gctcaaaaac cgatcgattc gcaaaatgtc tttctctttc acaaaaccac aaaacgagac
     3301 atgtatcccg ccccggtcta cgatgacatt ctgcttttca acgaaaaaga ggagttgacc
     3361 gaattcacca tcggcaatct tgtgattgag acggacgaag ggaaatttac cccaccgatc
     3421 tcctgtgggc tgttgggggg tacattccgc gctcatttgc ttgaaacagg gcaaatcagc
     3481 gaacgggtga ttcaaaagga tgaacttgca aactgcaaag caatattcct ggtcaactct
     3541 gtccgtggct gggttaaggt tgaaccagac ttctaaccac ttaacggaat atgccaaaat
     3601 taaacaaaaa tgcggcatct ttttcaagat gccgcatttt tcaaatatcc tattgctgat
     3661 aggcaaccgt aacagaagtg gatccatcgc ccacaggtgc gccgccgatc acaatggtct
     3721 tgccgttgtt cacgaagaac atcgcaatca taccgctaaa gtttgtcgtc atgctggtgt
     3781 cttcagtgta gccctgcttg cccaattcat cacgataaaa cttcatcaca tcatcaccgc
     3841 tcatattggt ctggtacgtc acagactggg gggtgctgat gacgttgaaa gcgtcgctgg
     3901 tcacagggaa gggtgattcg ccgcctacgg tgatatcgcc gccatcctgt tcaacagaag
     3961 ggacttccac atcatccccg tccgtctgtt gcggaacatc gggggcttca aagccattgt
     4021 cgccgccacc catgatggtc tggcaggcca gtgaagctaa aacaagagcc acaatcgcaa
     4081 gcaaaatact atattttttc attctcgtct ccttgagtta agtttcagtc attatacata
     4141 ccccatcagg aatgttaatc cccttaaagt ataatttatc gcatcatgaa cagactttcg
     4201 caagccacct cgccttattt gcttcaacat gcccataatc ctgtggactg gtacccatgg
     4261 gggagcgaag ccattgaaaa agcaaaagcc gaagacaagc ccatttttct cagcattggc
     4321 tacgccgcct gccattggtg ccacgtcctc gcgcatgaat catttgaaga ccccgaaacc
     4381 gcggccttca tgaacgaaca ttttattaat atcaaagtag atcgcgaaga acgccccgat
     4441 ctggatggca tttacatgca ggctgtcgta gccatgacag gctcaggcgg ctggcccatg
     4501 tcggtctttc tttcgcacga tctcaagcct ttttatgcag gcacctactt ccctcccgta
     4561 cgccgcttca acctgcccgc cttcatggat gttctcacag gccttgcaaa cgcctggaaa
     4621 aacgaccgcg ccgagatcga aaaggtcggc gaacaagtca atgggcactt gcaataccaa
     4681 aaccaaagca aaaatacaga agcgatcaca caggaacacc ttgaacccat cgccaaggct
     4741 ttgcaaggtg cctattattg gggatacgga ggttggggcg acgcgccaaa attccctcag
     4801 cccatggcgc tcgaattcct tttgcatcat tccattgcga acaaaaagga tgaatacctc
     4861 aaactcattg accattgtct acgcatcatg gctcgcggcg gcatgtacga tgtggtcggt
     4921 ggcggctttt cacgctatag caccgataac ttctggcggg tgcctcattt cgaaaaaatg
     4981 ctttacgata acgccctgct cgcccgcgca tatctgcatg catggcaggt aacaaaagag
     5041 cctttcttca aacagatcgt tgaagaaacc ctcgggtttg tcgctcgcga actcacccac
     5101 gaacaaggcg gcttctactc ctcgctcgat gccgactcgg aaggcgagga gggcaggttc
     5161 tatgtctgga ctctcgatga gatccgcgct gttctgaaag atgattcaga gtttttcgaa
     5221 gcggcatatg ggattagtcc acagggaaac tgggagggca aaaccgttct gcaacgcgca
     5281 ttagatgaca caacactcgc cgcccgcttc aaactggacc ctgaaactgt tcccgccaaa
     5341 ttggcagaat ctcactccag gcttttatca gcccgtgcct cgcgcatccg ccccggtacc
     5401 gatgacaaag tcctcactgc atggaatggt ctgatgctgg cggctttcgc cgaatcggcc
     5461 cgagtcctcg caggatcctc cccgcattcc cacacacatc ttgatctggc tacgcgcagc
     5521 gcagagtttt tagtcaataa cttgcgtgtc aatgggaagc tgcaccgttc ctggcgcaac
     5581 ggacaagtaa ccgaagaggt tttcctcgaa gattacgccg ctctaatttt ggggctgctt
     5641 gagctatacc aaaccgattt caacaataaa tggtttacca tcgcccatga attaaccgat
     5701 gaaatgatcg aaaagttcag caacccagat ggcggattct tcgatacacc gcacacaggc
     5761 gaaaccttgt tattccgccc caaagacatc agtgataatg ccaccccgtc tggcaatgca
     5821 ttggcttgtg aagccttgct gaagatggcc gcttatacag atcagggaag ctatcgcagc
     5881 cttgccgagc aatcgcttgc tttgatcgga gaatccattt tgcgatatcc gcttggcttt
     5941 gctcgttggc tgtcagcggc ggagaatgct ttgggtacga tgaaacagat tgcggtgttg
     6001 ggcgaagcgg gggaggaaaa tttcgagcgg ttgctcaagg tcattcgagc agaatacagg
     6061 cccggcgtgg tggtggccgc atcctcccat cccccatcac agcatgcacc tgccctgttg
     6121 gaaaaccgac ctctgctgaa cggcaaagcc accgtttatg tttgcgaagg ctttgtatgc
     6181 aaacaaccaa caaatgaaat tgaagtactg atcgctcaac ttaaagcatg attgaaagcc
     6241 atgctttttt gaacgcacac aaataaaaac ggacggtacg catggaaatg ttttatgaaa
     6301 acctgatcaa aattttgctg gcggttgtag ttggcggtgt gatcggtgca gagcgtgagt
     6361 atcaagacaa agcggctggt tttcgcacca ttattctgat cacactcggg tcggctttgt
     6421 tcacgatctt ctcggtaacc atggacccag gcttcacgcg ctcacgcgtt gcggctaaca
     6481 tcgttacagg cattggcttc cttggagcag gtgcgatcgt acgtgaaggc ggcaagatcg
     6541 gcgggctgac caccgccgcc accatctggc tttcggctgc attgggcatg ggaatcggcg
     6601 caggtgatgt gtacatctca cttacggcca ccgtcatcac cttgctggtg ctgctggtat
     6661 ttccgcgtat tgaagcccgt attgaccgtg tccgtgaggc acgttcgtat cgaatcgtga
     6721 tcggcgcatc gggcttccaa aaaatagaca aagtggaaaa gactctaaag gaatgttcct
     6781 tgcgtgtgct tgaacaccat cagtctaaat cggaaagcga aatcatcagc gtgtggaaaa
     6841 cgatcggctc tccgagcaat caggaaaagt ttgtgcagtt aatgctaaaa gataaggaca
     6901 tcagggaatt ggactattga tcttaggtga taaaaccgcc cacaacatca gttaaagaat
     6961 aaaggctcct gacctaggag cctttatttg tggttacttg ttttcttcct tcttttcagc
     7021 cgtatcagca gcctgttctt cgccggggtt caacccttca cgaaaagact tgatgctgcg
     7081 cccaagttcc cctgccactt tgccaatgcg acctggccca aacagcagaa caatgataat
     7141 gaggatgatg atccactctg caccacgtgg tagtcctagc ataaaacact ccttttttga
     7201 ttttcaacct tatcgtacca cagttgaatg aaaaaacaat taaagggatt tacgcgagaa
     7261 gaaaccttca atataacgcc agacgcggcg caaaagcgag cgcaggcggg caaagtagcg
     7321 atattgttcg gggtgaacat ttttctttgc gggtatgggg cctgtccatt gaatgaccat
     7381 ggcaccccag gttaagcctc cgccaaaccc gacaaacacg gctttgtcac cactcttcag
     7441 tcgtccattt tgaatggctt ccactgtggc aatcggaatg gaagcggtgg aggtatttcc
     7501 gtatcgatct acattaatga cgaatttatc cagtggcatt ttgaggtatt tggccgcagt
     7561 ttcaataatg cgataattgg cctggtgcgg aataatgcaa tggatatcgt ctgtggtcat
     7621 tttcaattgt tcgagagatt ccatcacagc gcggcccatc acgcgggtgg caaagcggaa
     7681 cacttccttg ccatccatgt ggacgaagtg tttgccttca tgaatggtta attcagaggc
     7741 agggaaatgc gaaccgccgc caggcagggt caatgtatct gcaccggaac catctgaatg
     7801 catcactgca gagagcacgc ccccgggcac atcactggcc tgcaaaacaa aggcacctgc
     7861 accgtcacca aacaaaatac aggtaccccg gtctttccag tttacgaacc gcgaaagcac
     7921 ttctgtccca accacaaggg cgttcttgat cgatccactg cgaatcgcct gggccgccat
     7981 attgaccgca aaaataaagc ctgtgcaggc agcctgcagg tcaaatgcac ccgccttggt
     8041 tgcaccgatc tggtcttgaa tgatgcaggc cgtggcaggg aaaatatact caggcgaaga
     8101 agaggtgcaa atgatcagat ctaactcggt gggagcaagg tttgctacct gtaaagcctt
     8161 gaccgccgcc tccacaccca ttgtggatgg aaattcatgc tcacgggcaa tgcggcgctc
     8221 acgaatccct gtacgctcac gaatccactg atcgttggta tcgaccattt tggaaatatc
     8281 atcattggtg aggacaggtt cgggaacata catgccccaa cctgtaacat gcgcgaatgg
     8341 cattctttct cctttacttg ttgaagcggc ttagaataag ggttgcatta tgcccgccaa
     8401 atccaaatga gttggacatg acgtggttcg gctcggcttt gcgaggttga ttgggaacat
     8461 aatcaagatc gcacacaggg tcgggggttt catagttcat ggtgggaggc aggatatttt
     8521 ccataattgc catggcacag aaaacagcct ctacagcccc ggaagccccg agcaggtgcc
     8581 cggtcatgga tttggtggat gaaacgggaa tcttataggc gcgttcgccg aaaacggtct
     8641 tgatcgcact ggtttcagat ttatcgttca aatatgtgga ggtgccatgg gcgttgatgt
     8701 aatcgatctc ctccagcttc agaccggcat cttccatggc aaggctcatt gaaccaactg
     8761 ccccggcgcc atcttcagca ggagcggaaa tatgatgagc atcatcggtg gttccatatc
     8821 cgctgaactc acacaaaatg tttgcgccac gcgcctgggc aaattccagc gattcaagga
     8881 tgaggacacc cgccccctca cccatcataa aaccatcccg atccttgtcg aaaggacggg
     8941 aagcccgctg gggctcatcg tttcgtgtgg aaagagcggt cattgaattc atgcctgcca
     9001 tggcaatggc attgatggca gcttcagaag caccagcgat catgacatcc gccgcgccgc
     9061 gccgaatcat ttcggcggct tcacccaggg cgttcgtgcc agaggcacag gcggtcgcca
     9121 aagacatatt cggtccacgc acgccgagac ggatggctat attcccagcc gcaccatctg
     9181 agatcatcat tgggatcagg aacgggctga cacgttcggg gccgcgttcg cgcatgatgt
     9241 catattgctc cagaagggaa ctgataccgc cgatgcctgt tccgatcaaa atgccaatac
     9301 ggtcgcggtt cgattcgtcc actttcaggc ctgattgcgc catcgcttcc aatgtggcgg
     9361 ctgtggcaaa ttgtgtgaag cggtccattt tgcgtgcgtc gcgcgttcca aacaacgcag
     9421 ccgcatcaaa accctttact tctgctgcaa actttgtctt atgcgcactc gcatcaaagg
     9481 ttgtaatggg agccgcaccg gattttccct ccagcagcgc ctgccatgta tcttttgtgg
     9541 tatttcctac cggactgaca caacccagcc cggtgacaac aacgcgtttt ctcattctag
     9601 ttctccttga attcaagcag caattttata aagtttaatc aaaagacgag gacaagttcc
     9661 acacttatcc tcgtcttcat ataacactat ggcggggtgg aagacacgcc gaaacattag
     9721 agtttcttca gcgttgtcca tatttgctgt agatgcatcc gatcatgatc ggccataaaa
     9781 ctgacaactt cgagaaaatc ggtggggccg aaaatggcat ggcgagccct gcggccccat
     9841 gcctcggcag aaacctcgga gagcagggcc aatgtttcgc gccgcgcatc gttgaattcc
     9901 tttagggcct gagggccgtt ttcatgcaaa taatcacgct ggctggccca cacacccgta
     9961 tccgggcgcg gaataaaagg ttcgctctgt tcagcaaaca acctgatctg catctggtga
    10021 atttcgcgct ctgtatcacg catatggcag ataatttcga tcatcgccca atcttcatct
    10081 gaatgttcgc gaggccaggt ggtttcatcc aaaccactta gcaggctgtt catggcggca
    10141 ggggttgcag agagaattcc aaggacggca tccttcgatt tgaaagatgg gatcagggcc
    10201 gaaaggtcag tggaatcaag ccaacggcgc agatcaacta aattgccgcg caaccccgct
    10261 tcaggtccag actgggaagc gggttcattt tccacgaaaa atgcagccag cccaagcatt
    10321 ttcgatggct tgatgtcacg ttcaatatcg tttcccacca tcaacacagg aacttccggc
    10381 cagcccatac gcccaagaat ttcagcataa taggcgggat gcgttttcga aaaatgaaaa
    10441 tgttcaaagg atgaaacgat ctcaaattgc tcaggctcaa acccagccca acgcaatcgt
    10501 gaatgagtgg ctttgagagg aaacagcgga tcggtggcaa tggcgatgcg gtaccctttt
    10561 gaaagcgccc attgtacgaa ctcaagagct tccggccttt gcgaggttac gttccccagc
    10621 aatggaaaga catggtcgta aaaattttca agaggcgggc ggagcgtttc gggagaagca
    10681 tccatgcgtg gataaaattc tgcgttgaaa acctgttcaa gggtacggga aaaatcgtca
    10741 ctcacattca tggcattggt gccagccatc agggcaggca gcatggccgt tggggttaca
    10801 tacgtgctca gttcctttgc aagggcttga aaatatgccg gtacaaaggc ttccatattg
    10861 gtgttgagca gagtgtcgtc gagatcgagc aggaggattg gctttgtcat attcttggac
    10921 tcccccgcgg agtcagatgc tcattctttt atttcaaatt taaagggcag atcgtggcat
    10981 tttccacagc cgatttgcgg ctcggaaaca ttttgatttg atttttcaca aaatgcgctc
    11041 acctgcaccc gccgctggaa catggcgagg atgggacgtt ccactgtgac tttcaattgc
    11101 atgtgctggc atgagtttgc acgggcaatt tcaggcacag ggcaggtttt gcacaggtca
    11161 cgcgtccagg cctgaccgtg cagtttgagc agccgacatt cttcatcgtt tcggccacga
    11221 aagtaattgc cataaaaatg tgggcattca cgccctgcgg gagttttcat tttatataaa
    11281 aaaacggagc gcaagtttca gtctgtgctg tgcctgggac caaatccgcg ctcctgtgat
    11341 ttgcaatggg tatcagtgtg gggttcaggg caagcctgag gcaggtttac acgcgggtga
    11401 cgtaatcacc agtgcgggta tctacgcgga tgacatcacc gacaacgacg aagttggggc
    11461 attgtacgac cacaccagtt tcggtaatga cctttttggt gacgccggtg gcggtatcgc
    11521 cgcggatggc catctcagct tcgacgactt tcaggtcgac cgaggtgggc agttccacat
    11581 caatggcttc acctttgtag aaggtgagct tgacttccat gccttcggta aggaatttgg
    11641 cgctatcgcc gagggattcc ttcttgattc cgggctgttc gaaggtggca ttatccataa
    11701 agtagtagag atcgccatcg ttgtataaat aggatacgtt ggcaaaatcg agggacacat
    11761 cctgcactga ttgcccggag ttgaatgtgc gctcaatgtt ggcaccagta agcaaattgc
    11821 gggccttcac tttgatgttg gcgttgccgc ggccagtttt gttgtggctg taatccaaca
    11881 ctttgaggag gttgccgtcc aattcgaatg tgacgccctt gcgaagttca tttacatcta
    11941 tcatgcttgc actttccttg attaattgat tttgtttttt tatacgagag cgggcagatt
    12001 ataccaatga gttgtaaatc gtggctaggt tggtctgtag gagcgggtgt gctccactaa
    12061 tgggaggctt ctgaagcagg gtcattgctg aggagttgca cggcatgggg aagaacagac
    12121 aggatggcct gcaaattttc cacagcgcct ttggggctgc caggcaggtt gacaatgagg
    12181 gtccgtccac ggatgcctgc tacagcgcgg gaaagcatgg catgggcggt ctttttcagg
    12241 ctatcggccc gcatggcttc ggccaacccc ggtgcctcac gctggataat ggcgcgggtg
    12301 gcttccgggg tgacatcgcg cggggcaaag cctgtgccgc cagtggtgag gattatgtcg
    12361 aaacgtccgc tgtcagccca ggtgaccagg gcggtacgga tggcgggttc gtcgtcgggg
    12421 aagatggctt gttcgacgac cgaccagcct tgagcgtgga caagatccgc aagggcgggt
    12481 ccggaggcgt cttcgcgctc tccacgtgca gaacggtcgg agagtgtcat gattccaatt
    12541 cgaatcgcca tatttgcttt ccaccgtaaa ggttaggcgg cctgcaaaac ctgcttgaga
    12601 attgtgataa ggtcatcggg catgtagggt ttcataagga aaccatttgc gccacggcgg
    12661 gcacattctt cgcggacatc ggagcctgac gacatcacga cgcgcgtggt ggctgtctct
    12721 gtcgcttccc ggatatcgga gaggatatcc agcccgcttt ggccgaagag atgcacatcg
    12781 agcaaaacga tatcaggctt ttcgtttcgc acagctaaag gcacatcggc atctggctgc
    12841 aaagcaagca cttcaaagcc ttccattttg agcagggttt tcaaaaggga taccatggtg
    12901 atgtcatctt cggcgattag tacttttact tttgccattt ttctttactt tcgtgggttg
    12961 ggattttttt ctgcgcggct taccggcctt cttttcctcg ggcttgttct tttccaatgc
    13021 ggcattgatc acatcttcga cagactcggc aaaaataaat ttcatggatt gcctgatctc
    13081 gtcagggaca tcgtcaatat cggtttcgtt gcgttttgga aggatcacgg tagtcagacc
    13141 gttgcggtgc gccgccaata ctttttcctt gacgccgcca atggctaaaa cctgacctcg
    13201 caatgtgatc tctccggtca tgcctatctg tggcttgacc ttgcggccag agatcagcga
    13261 caccaaagat gtagccatcg ccactcctgc tgatgggccg tctttgggtt gtgcgcctgc
    13321 ggggatgtgc atgtgcaggt catatttgtt gaagaattct tcgggcacgt ttagagaagc
    13381 agcccgtgag cggacatatg agagtgcagc ccgggcagat tcctgcatga cgttaccaat
    13441 ggagcctgtt acctgaaagc cgcgtccacc gggcatggca gttgtttcga taaataagat
    13501 atcgccgcca aaagaggtcc atgccaggcc ggggaccacc ccggggatgg aggtgcgttt
    13561 attgagttcc tcggtaccta aaaagatggg atggtcgagg tattcttcca ccagctcggg
    13621 cgtaatctca aatttttcac ctttgtgttc ggcgatcttt gtgcccactt tgcggcagac
    13681 tgcgccaatc ttgcgttcaa gattgcgtac accagcttca cgtgtgtact gacgaacaat
    13741 catacgcagg gcatcatcgg tgaaagagat ctcttctgcg cgcaggctgt tttcacgaat
    13801 ctggcgcgga atcaaataac cgcgtgcgat ggacatcttt tcgttttcgg tataacccga
    13861 aaggaagatg atttccatgc ggtcacgcaa ggggccagga atggtttcaa gcgtgttggc
    13921 tgttgtgata aagaaaacct gcgagaggtc aaaagatact tcgaggtagt tatcacgaaa
    13981 ttcactgttt tgttccgggt caagcacttc caggagggcc gatgcggggt caccgtgaaa
    14041 atcgtaggtg agtttatcca cttcatcaag catgaagaca ggatttttcg aatcaacgcg
    14101 gcggagagat tgcaggatgc gcccgggcat tgcgccgatg taagtgcggc gatgtccgcg
    14161 gatttcggct tcatcacgca ctccgcccag ggacgcacgg atgaatttgc gttccattgc
    14221 acgggcgatg gattgcccaa gcgatgtttt ccccacgcca ggcgggccta caaagcaaag
    14281 gatcacgcct tcgcgttcac ggcgaatcag atcgtctgaa ggggctttct catcgtcctt
    14341 gcggtcaatg cgcaatttgc gaatggcaag aaattcgaga atgcggtctt tcacatcttc
    14401 gaggccgaaa tggtctttgt tcagaatctc acgggcgtgc gcaatttcga ggttatcctg
    14461 cgtggatttt gaccacggaa gcgaaacgag ccagtcgaga taggtgcgga tgacgccata
    14521 ctctgcggca gccgtgggaa gacgggaaag gcgatccaac tcctgtttgg cctgtttatg
    14581 ggcctcctca ggcatgttcg cctcttcgat ctttttgcgg aattcatctg cttcgacaga
    14641 ttgctcatcg cggtcgccca gctcgcgttg aatggctttc atttgttcgc gtaaaaagta
    14701 ttcgcgctga actttttcga tctcgccgcg tgcttcgttc tggatcttct gcccgatctg
    14761 cagcacttcc gcctcgcgga cgagcagtcc aatgagcttt ttcaattttt catacactga
    14821 atccagctca aggatctcct gggcatcctg caaattgatg cgctgaaaat tggcgatggt
    14881 ataaaccgtt tgcagagggt cttcgagcga ggtaattgag cccaccagtt catcggggaa
    14941 cgaggggatc atttgtgtga tctgctgaaa ttggtcgcgg gcgttacggg ccagtgcgtt
    15001 ggtttcgaga ttcgattcca cggtttcggg aatcaggctg atgcgtgctt tcaagtaggg
    15061 ctcgtcggcg acgaactcct ccacgcgaaa acgttccatg ccctgcacca acagccgaat
    15121 ggttccatca ggggcacgga gcaggcgatg aaccgtggca atggttccga tttgatacag
    15181 ttcgttggga ccgggggttt ccagctctgg gttgagggcg gccacaaggc ccaccaattt
    15241 atcgccgccg acaacatcgt ccaccagttt gatcgagcgg ggttggccaa cggtcaatgg
    15301 gacagcggtg ttggggtaaa caaccactcc gcgcaacggc agaatgggaa gtgtgtcggg
    15361 gaatttctgt ccctcgtttt tgtcatcagg ctgcggggcg tgtgaagcgc catcgtcttt
    15421 ttgaaaacgt gccagggccg aagtcatgaa catttttatc atttcttcgt cggtctcgcc
    15481 catggattgt aaaaactcat tgattccggt ttgccaccgt tctgcgggca tatgtttatt
    15541 caaccttgat ctgttttggt gttgcttttg ggagggtgac ggttagaaat ccgtctttgt
    15601 attctgcaag tgcgtgttcg atatccactg gtccgggcag gcctaatgcg gttgcgaact
    15661 tgccaaaccg aatttccatt tgatggtagg cgcgccgttc ctgggcatcg gtacgcaccc
    15721 ctgcgatcag cagggtattg ttgttctcta cggaaacctc aaaatcatct tcattcatgc
    15781 ccgcaatttc aacgcggacc acatatgtat cttcggtttc gtattggtca gtgggagggc
    15841 tccaaacatt tgagcggaca tgccaactca ctgcatgaag gatttcacgg cgcgtctcca
    15901 acaacgctgc gctggattta cgtacgatcg caggcatgga aattctctcc ttcactatgc
    15961 gtttgatgga accatgtggc gcctccgccc agattcgaac tgggaaccta ctgcttagaa
    16021 ggcaggtgct ctatccattg agctacggag gcgttttaaa actactcgcc tgattccgcg
    16081 attggtaggc caacctcaaa gggtctcaaa ccacgataca ctcgcggacc tgcgatcgtc
    16141 ttcaatatgg tgtctgcggg gcggccaagt ttttggctca gttcctcggc agacatgggg
    16201 gtattgccat ttacaagaaa ggttcgaaat acggcttcca ccatggcggt gcgtgcatcg
    16261 gaaaactcgg gcagcaaggc acaatggctc atcagggcat gttggacgcc agccacctgc
    16321 cgtacttcag cggtttgcgg gtcgatccag tcgatcatgc ccccttcgtc catctcagca
    16381 aaggcctcct gatgttcggg gcacaacaga gaacgtaaaa acacatgcca atcgcgttcg
    16441 tttttcttcc accattcaaa gtcaatatga aaaggtgttt tagcgttcgg ttttagtaaa
    16501 ctcattcggc ctcctcatcc aggcggaaat gcaggtcgcc tacatgtttg aaggcagggt
    16561 tgctttccaa caatgcaaaa agcggggcag gcggaacacg gcgtacgagg ttgatcgccg
    16621 catacaattc ctgcgcgtga acgtgtccct gcggattcga tttggtcagt tcgcgcatga
    16681 cggacaaaac aagttcgttg aacgactgtt tcttctccca aacagggtcc agggatttga
    16741 aatcagggac atggataacc atgcggtcat taaactctgc ggtgatcggc tgcttcagca
    16801 tggcaaaaac aaacccgcca tccttgccga ccagcacagt acgaacccaa tcctttgagg
    16861 atcgctggct ttttacttcc acgatcactt cgccaggctt ttcaccgcgc ttcaactgta
    16921 cgatcgaacc tgggatcagt tgatgggctt tataccattc acgcaacccg aatacatatc
    16981 catgcttcaa tacaacccag gcagggatac tttgcttggt tttaccatcc acaagggtaa
    17041 aacgcacacg cgatgattca tatgctgttg ggaacaacct gcgcgcccgg gctgacatgg
    17101 ggagcgtccc tgcacgcagg tgcgggtaaa ttaaggtaat cgtaatggat gaaatatcgc
    17161 cacgggcatc atcttcatcc accggggtca attcatcatc caactggtct tcgaggcgtt
    17221 taatttcatc gccaagcacg ctgcgatcat gctcaatctc tgaataccga agcggccatg
    17281 gcacttcacg gaccaaatca ggctccagac gatacaaaca ccacaacacc tgtcctgccg
    17341 gccccacctc gtcaaagcgt tcatcgctct gcaaagcgta attcagggag aactcggcca
    17401 atttcgggtt aacacttgca gggagttcaa tatccttcat cagtgcttca gtggtaagag
    17461 gctcaccacc agccatatcc aaaacggcct cagcaagatt caactgtccc tgattaatat
    17521 cgatcaaaag cgcacgcggg aaccagctcc cggcaatctt aaccagcccg ctatcagcgg
    17581 taaacgaggc ttcaagtttc ttttcgatct gctcgccaaa ttcgcgcaag accatctctg
    17641 cgctcatctc gttcgtctca ggctcaacaa tcggagcatc gttcaggtag tgggcttcaa
    17701 ggttcgctgc gaacatgcgc tcgacacgat cttccatcgt aaccgtgagt acatcaaaac
    17761 ccgttacaga agggtttacc ccttcacgca acgcggtaac cgtcccgcgt ttccagtcta
    17821 atgcagggaa tataagttct tcaccaactt gatacttctc ttttggaaga taactcctgc
    17881 ccgcagcctt cttctttccc tcagccgctg cacgctcggc cttgatacgt gtttcaacaa
    17941 gttcaacgga aagatcccgc gtcgtcagcg gtgtttcgcg ctcaaaaaga taattatgca
    18001 gattttcaac atcttgtgga gtaatctgaa tggataacca atattcgttg gggagtgaaa
    18061 atgcgcctgc catactcagc cttgaaatga atttacgctt tacgcgtgat aaatggatta
    18121 taccttacct tgcagatact atctatattt ttgaaaaaaa gccgccagag taagaaacca
    18181 catgatttcc acatcaaatt cacctgataa tcagttaaag tttgccccac gcaaacaatg
    18241 aaaggaactt agaaatgaac aaggctttaa ttttcagttt gaccctcttc accattttga
    18301 tcgctgcctg tgggacacaa gcgactcaga ctccgccccc acctgccact gtcaccgaag
    18361 caccaaccat ccctgcggct actgacaccg ccatccctgc cccaaccgaa gcaccgattc
    18421 ttcaggcaga gacaccagcc caaacgcagg aaacagccgc cacggtcagc ttcgcaaaag
    18481 atatcatgcc cattttcgaa gccagctgta ccaaatgtca cggtgttgaa caggtcaaag
    18541 aaggtcttga cttgcgaacg tacgaaaccc tcatggcagg atcattcaac ggcagtgtca
    18601 tcacccctgg caatgcaaat gacagtttcc tggtagaaca gatcgtcgaa ggcgaaatgc
    18661 caaagcgtgg acaaaaacta ctccctgcac aaattcagct catcattgat tggatcaacg
    18721 caggcgcact gaataactaa ccaaatcaaa gcgccaatta cgaaaacgac ttgaggtttt
    18781 cactcaagtc gttttctttt ccttgatcca caactttcac atttgcaact cacaaatact
    18841 tgacttttcc caatacgcgt cgtaaaatct tattaatctg attaatcaga ttacagccat
    18901 ggttttacgc gaccgcatct ctcccgaact ctctgaattc ctgcgctatc tggcttcaca
    18961 tgaagaagcc actggcggac ttccatcgtt aaacgaccta agccgcgagt tgggcatcag
    19021 ccttgccgcc ttacgcgaac aattggaagt tgcgcgagcc ctgggtctgg tcgaagttcg
    19081 tccccgtacg ggcacaagac gcctacccta ctcttttgcc ccatcggtga tgaagagcct
    19141 tgcatatgca ttaacccttg atcaaagcaa cttcgaaaag tattcggaat tgcgcaatca
    19201 catcgaaacc gcatattggg atgaagcggt atcgctctta accgaggaag acaagctgga
    19261 attaaaaaac atcaccgccc gagccttcga aaaattgcat gcaaaacaag tgcaggttcc
    19321 acacgaagag catcgcaagc tgcatatgct gatttacagc cgcctgaata atccctttgt
    19381 tacgggcttg ctcgaggcct attggaatgc atacgaggcg gtcggcctga acatgtacgc
    19441 aggcggcatt gattatctcg aagaagtatg ggggtaccac aaaaccatgg ttcaaagtat
    19501 ttgcaatgga aattacgcgg cggggcgcaa agcgctgatc acacacattg acctgctcgc
    19561 ccatcgcccg acataaatta aggagtcaca tgaataagat catcaacgat ttttccatca
    19621 cagttggcac cgtgaacggc tcaggcagtt ccaccgccaa caacaccatc ctgcgatcca
    19681 tcttcaagat gggcataccc gtctcaggca aaaacctctt cccttccaac attcaaggcc
    19741 tgcctacctg gtacaccatt cgcgccaaca aggatggatt tatcgcccac aacgaacgcc
    19801 gtgacattgt cattgcgatg aatcccaatt cgttcgtacg tgacctggca tctgtcaccc
    19861 ctggcggagc atttttctac gccgacgata tcaagcagcc catcaaccgc acagatatcg
    19921 ccatttaccc catgccggta aaaaccatcg taaagaatga ccccaatgtg cccaccgatt
    19981 tccgcgatct tgttgggaac atggtttacg tcggtgttct ggcgcaaatg atcaatctcg
    20041 acatggacaa gatacttgca gcgctgacct tccacttcaa gggcaagcag aaacccattg
    20101 acatgaacta tggcgcggtc aaagcaggtg cagaatgggc caggagcaac ctcgagaaga
    20161 aggatccatt ctacgttgaa accatgaaca agaccgaagg catgatcatg gcagacggaa
    20221 ataccgcagc cgcaatcggt tccatttttg gtggtgtgca gtttgccgca tggtatccca
    20281 ttacccctgc aacctcactc gcagaatcac ttaacgatta tctcccccaa ctccgcaagc
    20341 gcgaagacgg taaacatacc tacgccgtgg ttcaggcgga agacgagctt gccgccctgg
    20401 gtatgaccgt cggtgctggt tgggcaggtt tacgctccat gacctccacc tctggcccgg
    20461 gcctgtcctt gatgaccgaa ttcgctggcc tcgcctacta tgcagagacc cctgtggtca
    20521 tctgggatgt gcaacgcatt ggccccagca caggcctgcc aacacgcact tcacaaggcg
    20581 atttgacctt cacccatttt atcggtcacg gtgattcgca ctctgtcacc ctcctgccag
    20641 gcgatgtatt tgaatgtttt gagttcggct ggcgcgcatt tgatgttgcc gaacgcatcc
    20701 agactcctgt ctttgttctg agtgacctcg acatgggcat gaaccagtgg atgagcaaac
    20761 cttttgaata tccatccacc ccgatggatc gcggcaaggt cttgttcgaa aaagatatcg
    20821 aggaactcaa aggcaattgg ggccgttacc tcgataaaga cggtgatgga attggtttcc
    20881 gcaccttccc tggcaacaag cacccgctca gctcctactt tacccgcggc acaggccacg
    20941 atgaatacgc caaatacagc gaagaagcgg atatctacct caaaaacatg gaccgcttga
    21001 agagaaagca tgagaccgcg aagaaatatg tccctgctcc agtgatgcat tccataaaag
    21061 gcgccacggt tggcatcatt gcctatggct ccaccgaagc agcagttgtg gaagcccagc
    21121 accagttgga aaaggatcat ggtatcaaga ccgatttcat gcgcgtccgt gccatcccct
    21181 tcaccaagga agtggatgcc ttcctcgcaa aatacgacca aatcttcatc gtggaaatga
    21241 accgcgatgg ccaaatgtat caaattctgc tgacggaata tccgcaattt gcgaaccgct
    21301 tcaaatctgt ggcttttggc gatggcatgc ctgcctctgc caaatgggtc cgcgaaggaa
    21361 ttcttgccaa atacgcgaag gttggaacgc ccaagaaagc cgcagcggca aaaactgcgt
    21421 cgaaggcaaa agcagtttca gggtccaaaa aagcagcgac taaaaagcca gcggcaaaga
    21481 aacccactgc caagtcaaag tcatccgtga agtcgaagcg gaagtaataa ctttatcaag
    21541 gagcatataa aatgacagaa gctctcccca tggccggcac acctgccaca aatgtcaacc
    21601 tcgctggctt atccaaaaat gaatatcgcg gcaacccgag caccctgtgc cagggctgcg
    21661 gacataactc gatctcaaac caaatcgtga ccgcgctgta tgaaatgaac atcgtacccg
    21721 aagacgtggt gaaattcagc ggcatcggct gttcttcgaa gtcacccacc tacttcctga
    21781 accgctcctt cggcttcaac agtctgcatg gacgcatgcc gtccattgca acaggcgcgt
    21841 tatttggcga ccatcacttg aagggcatcg gcgtttctgg cgatggtgac tctgccagca
    21901 ttggcatggg tcagttcaaa catgtgatgc gccgtaacgt gaatatgttg tacattgttg
    21961 aaaacaacgg cgtgtacggc cttaccaaag gccagttctc tgcgaccgct gaaaaaggcc
    22021 tgagcctgaa gaagcagggt gaaaatccct acatgcctgt ggatatctgc atggaggcat
    22081 tggtctcaaa tgcgaccttt atcgcccgtt cctttgcagg caaccccaaa caggtaaaag
    22141 aattgctcaa ggccgccctt gctcatgatg gcattgcagt gctggatatc atcagcccgt
    22201 gtgtcacctt caacaatcag gaaaacgcct atcactctta tgcgtggggc aaggatcacg
    22261 aagaacccct gcatgaaatc tcctacattg cccctcgcaa cgagatcatt ctcgaacgcg
    22321 agatggaaga tggcgaagta cgcgaagtag ccatgcacga cggctcaacc gtgatcttga
    22381 agaatctcga aaagggctac aaccccacca accgtttcga agccctgcgt gtgatggaag
    22441 aagctggccg caacaactgg ctgatcacag gtttgttgta tgtggacacc tccaagccca
    22501 acctcacttc aatgtacaac cttgtcgata caccgctcaa ccgccttacc gaagccgaat
    22561 tgagacctga ccgctcaatg atcgacaaag tcaatgcgtt gatgttctaa cacacccccc
    22621 gttcccaccc taccctcccc caaatcccaa aagaggattt gggggagggt ttataaaatg
    22681 agaaaccatg accacagaaa caacaaacga aaaacttgat ttcaaacgca ttctgcctgt
    22741 attagtgatc gtgctggtgg atttgatggg cctgtccatc atcatcccgt tgctgccgtt
    22801 atacgcggcg cggtttgggt cttcgccatt ggtgatcgga attttgcaag ccacctatcc
    22861 gatgatgcag tttttaggag cacccattct cgggcgcctc tcagaccgct tcggacggaa
    22921 accaattctg gtcgtaagcc agatcggcac tttgatcgga ttcatcatgt tggggtttgc
    22981 aaactcgctt ttgctgttat tcatctcccg cattattgac ggcctctctg gcgcgaacat
    23041 ttcaacagcg caggcagcca ttgccgatag caccaccgaa aagacccgca cacaggggct
    23101 ggggctcgtg ggggcagcat ttggcatcgg ctttgtactc ggccccatcc ttgcatttat
    23161 cgtacttgcc gccacgggtc aaaactacca ggcagttgct ttcaccgccg ccttcttttc
    23221 gctggcctcg atcctgttaa ccttattctg gttcaaggaa acctacgcag aagttgaaaa
    23281 ccccgaagcc tcccgcaaac gccgcccatt ttcattcggc gcgttacgtg aagcgctcag
    23341 ccgcccttcg atcggttttt tgctcattct gatgttcttt tatcagatcg catttggcgg
    23401 gtatgaacag cttttctcat tattcacgct cacccgcctc ggcatggatg cacgggatac
    23461 ttcgggcctg ttcgtgctgg caggtttgtt catcatcgtg gtacagggcg gcttgatcgg
    23521 cagatggtca aaacaaaaag gtgatcgctg gctggtgctg atgggaatcg gcacacttgc
    23581 cattggtttg atcggaacgg ctctcacacc cgcagtgcca attccctggt atgaaaaagc
    23641 gaaggtgctt gaaagcatgg cggggcaggg tgagttccgc gtttcaattc aaagcatcaa
    23701 cattcaactc cctgatgaag cagcaaaagg ctggctcggt atcatttggc tcctgcttgc
    23761 aagtttccct gccgctttgg gtggtggtgt tctgcagccc gcagtgaaca gccttattac
    23821 aaaagcctca gataaaaacg aagttggcgg aatgctcggc gcatcctccg cagcgtatag
    23881 cgccgcaaac gccattgcgc cgctttttta tggagccctg ttccaatggt ttggcggccc
    23941 tgtcccattt ttcgcaggcg gacttatcct tgcgatcatg tggctgctcg ccccacgggt
    24001 agtgaaggca taattacgta gggacacagc ggcaccgctg tgtccctacg atataattcg
    24061 gggaaatgaa aaatctggaa tcggaaatct cactggaata tattcgcgca tcaggcccag
    24121 gcggacagaa tgtaaacaag gtcgcgacgg cggtccaact gcgctttgac attaccaact
    24181 ccccctccct cgcttctgac gtaaagggga ggttgattcg gctttctggg agtcgagtca
    24241 ccgctgaggg agtgctcatc atcgaggcga aaaaatatcg cacgcaggag caaaaccgcg
    24301 aagacgccct tcagcgattc tatgatttag tggcaaaagc aaacgaaaaa ccgaaggtgc
    24361 gtcacaaaac aaaaccaaca aaagcctcaa aagaggaaag gttgaaagaa aaaaagaatc
    24421 ggggaagcat caaaaaaatt cgacgcgaca aagcctacga ctgagccgcc atgtcgccgt
    24481 ccatcgccca tgtcacttac cttgtacgtg attacgatga agccattgcg ttcttcaccg
    24541 aaaaattaaa cttcatcctg cttgaagaca caccccttga cgacaacaaa cgttgggtga
    24601 gggtctctcc acaaggtgga aatggatctg ccttactttt ggccaaagca gacaccgccg
    24661 aacagaccca gcacattggc aaccaaacag gcggcagggt ggctttcttc ctccacacgg
    24721 acgactttca gcgcgaccat caacacatgc agtcgcatgg tgtgcggttc gctgaagacc
    24781 ctcgccacga gccctacgga atcgtggcag tgttctttga tctctatgga aacaaatggg
    24841 atttgcttga gccgagaaat ttgtagcaca atccgctaaa aaatattcta aaaagagaga
    24901 gctgtggact tttccttcat ccaaatcaca gatcaccacc tgactgactc cgacagtgaa
    24961 tttctgcgag gtttctcaac cagatatgct ttacgcacag ttctgcggca tattgctcac
    25021 aacgcaggga ctcacgttga ttttattgtt tccaccgggg atcttgtgga tatcccttcg
    25081 gaagcctctt atcaagcctc ccttgagcta ctcggtgtac aaaataacct ggcagaaatt
    25141 ccagggcctc tccacatttc agtggagggg ctgcaaaatt tgcccatgtt tgtgctgcca
    25201 ggcaatcatg atgaccgcaa gaatttttac aagtatttgt ttccccaaag ccaatcagca
    25261 tccatcatga atgcagcttt catacacaaa ggcatccaat ttatctgttt ggactgggga
    25321 ccagaatcaa aggcaacagc ccatcccgaa atgctggaat tcctcgccca aacaataaaa
    25381 aaggatatac catccattat catgatgcat caccagttgg taaaactggg aagcaaatgg
    25441 ctggacgatt ttattgcaga cgacacctcc cgtttctggg agatcatcgc tcagaaacct
    25501 gtactcggta tcttttgcgg gcatgtccat gctacgtatg aaagtatcgt gaacgaagtt
    25561 cccatttttg ggctgcgctc cacagcgcct caattcgttc tacaggatga gccgcttgcc
    25621 tgccttttac cgcctcatta tcgattggtt acaattcaaa acgacaccct gacaacccaa
    25681 attttcgaag tacctctata aataaaaact tttcaaggag aaacacaaat ggctgacatg
    25741 gcaaaagact tcatcaaagc agcaaagaaa gcggatctcg atgccctcaa agcattgcta
    25801 aaggctgaca agtctctgct caatgccaga gatacagatg gttccacagc attgcattgc
    25861 gcgacctgga aggggcatcg tgaggttgtc gcatttctgc tcaaagttgg ggcggatgtt
    25921 cacgcacaga acagcaatga ccactggggc accactcccc tccatgctgc tgcacatgca
    25981 aatcaggcgg ttctcgttca aatgctgatc gaagcaggtg cagatgtaag tgccaaggat
    26041 atgaacggaa aaactcccct gcatcacacc accttccata aagcaacagc ggcggcaaaa
    26101 gtccttgtgc agcacggggg caagtagaaa gcatgatcac agctcttgag gaattctcgt
    26161 taaatgcctg ggccgcactt caaaccgtcc attatgatgg ttgggtgctg cggtttgcca
    26221 acggatatac caaacgcgcc aattccatca acccgatcca tgcttcaagc attccgcttg
    26281 acgagaagat tcgattctgt gaggaaatct ataagagcag acaactccct gtagttttca
    26341 aactcactcc acatgtctac cctgaaatgc tggatgaaga actttcagcc agaggatttc
    26401 aaaaatctcc atcatccacc agcgtgcaga ccctgaaatt ggatttttcc gattttcacc
    26461 ttccgttaca aactgagctt gaggaaagcc tttcgagcgc ttggctgaat gccgtatgcc
    26521 atatgaatgg ggtcgctgaa gaaaatcggg aaacgctgcg acagatcctg ttaaatgttg
    26581 tgccgcgtca ttgtttttta tcacttaagt ttgacgacag gattgtagcc tgcggcatgg
    26641 gggtcttaca gtcagaattt ctcggcttat tcgatatcgt caccgacccc gccttccgaa
    26701 accgaggata tggaagacag gtggtggaag ggcttctggc gtgggggcaa caaaatcagg
    26761 ccaaacaggc gtacttacag gttgaactcc acaatacggt cgcactgcat ttgtattcct
    26821 cgcttgggtt caaggaacaa tatcaatatt ggtatcgcag caagccatag tcagcggggg
    26881 atcttgtcag cagggaaatg ccgtttgttg gctgaccagc cagcagttag ggaggattcg
    26941 ttttttcggg ggagatattc caaataatga gcgtaatgta taataccggt aagcccgttc
    27001 agattccatc tccaaaccca aatttaccgg tcgcgattca ccataaatcg tccattcgta
    27061 cgcattgtga ttttcaaagg cgtttttgtg cttaatgaat aaaaccagcc atttcttgaa
    27121 acctccaaag aacaatacac ccttattcca ctgggttggc atggggattg tattcacctt
    27181 tggggttttt tattatctgg cattggctgt ttttcttccg cgatacgggg aaggggtgac
    27241 catctttgtc gttctaccgg tggctgtcgc cgggtggatt tttgggaaga agatcggggt
    27301 gtgggttggg gtattcagtt ttcccatcaa cctccttttg cttgccctgg caaaccgtgg
    27361 acaatttgat cgggcaatgt cgcttcgatg ggcgattgaa attgactttt tcctcgcagt
    27421 tgcggcaggt tttacaagtg ggtatgcggg ggagatcatt caaaaaaaag ataagaatca
    27481 agattgggaa agtttcctca ccgccatgga agctgtgccg catggcatgt tgatcgctga
    27541 ttcgaatgga aaaatatcac ttgccaacaa acaagcggag aatatttttg gttatgaggc
    27601 gagtggctta cagggaatag gaattgagca gctggtgcct gaacccatac gccatcatca
    27661 tacctttcat cgtagtgatt atttaaacca tccccacccc cgccagatgg aagggcgtga
    27721 attgactgca attcgcaggg atggaagtac gttccccata caggttgggc ttaatccaat
    27781 ttggaataat gggcagatat ccatcctggt ttcagccatg gatatgagct tacaaaagca
    27841 ggcagaagcc cagcgccaat ccttgatgga aatcatgcag gccgcagtgg aaacgaataa
    27901 cctgcaggaa taccttgcaa aggtccatgc agcgctaaag aaagcattga gcgctgaaaa
    27961 ctactttgtc atccttaaga acaaaactac tgggatgtac gaaggagcat attcggtgga
    28021 ccagatcaat gcggctctgg ctccgcaatc ggagatgcaa aaaagcgcaa gcgcatatgt
    28081 gattcgaacg ggcaagtcgt tgctgttaac cgaggaaaga ttccagcagc taatagcaga
    28141 aggtgaaatg gagttgatcg gcagtcgttc cctctcgtgg ctgggcgcac cgcttaagat
    28201 cagcggtgaa tccatcggcg tgatggttgt gcaggattac gaaaaggaat tttgctattc
    28261 gaaggcggag gaggagttct tcacctcaac tgcgggacag gtggctctgg ccgttgaacg
    28321 gaaacgcgct gaagaggaat tgcgcgccag tcaacagcat ttttacgagt tgtttgaaaa
    28381 tgtacccgtg ggtatctggg aatcagactt cacctcaacc cgtgaatatc tcaatgagtt
    28441 gtccgatgca ggcgtgacag actttgccgc atatttcgac gcccatcctg aggcagtgaa
    28501 tgaatgtgtg agccgcatca aaattttgaa tgtaaatcag gcggcgcttc agttttacgg
    28561 cgtggaggac aaatcaaaac tgatcgataa atcgaccacg ccgatacgga tcgacagcaa
    28621 gagtttcagg gtattcaaag agcagtttat cgccttgata aatggaaaag tatcctacga
    28681 caccgaattt gaagcattaa acatgcgggg tgagatacga actgttacga tgtggctaag
    28741 gctcgcctac ggcgctgaac agacctggaa acgggtattc gtggtctttt ctgatattac
    28801 agagcgcaaa ttggcggaac aagccatcca aaccgctgaa ttccgatatc gacagatcat
    28861 ggaacagatc cctgccgtgg tatatagcgc cgggctggga ccagatatgc gttggaaatt
    28921 cgtcagcccg cagatcaaag acctgacagg atacaccgcc gaggagtgga tcgctgacca
    28981 gcaaatctgg cagcgcagcc ttcaccctgg cgatcgccag ctggtgaccg atgtggaaac
    29041 ccggtccatt atcactgggg agcctgttaa attggaatat cgcctgtata ccagggatgg
    29101 ccgcatgata tgggtccgcg atctggcgac cgttgcatcg gacgaaaacg ggaatccatt
    29161 tctttctttg ggtttcatgt tcgatatcac cgaacaaaaa ctggcggaag aacaggtccg
    29221 aaggcgggcc gatgaatttg ctctgctgta cgatactgca cacagcctcg cgaaaagcca
    29281 ggcagatcta cggtctgttt tgcaagtgat cgctgcgcgt gccgaatcga tgcttgattc
    29341 gaattcggcg ggcttttacc tgtacgatgc cacccgtgaa caacttgatc tggccgtcag
    29401 aatcggagag atgttcccag cgggaccagt gcggttgaag cccggtcagg gtgtcagcgg
    29461 gctggtggta cagactcgca aaacgatcat tgtcaacgag tactcaacct gggaaaatcg
    29521 ctcccccttg tttgacggtc ttaatgtgaa ggccgtgttg ggcgtgccga tggtctatgg
    29581 cggggaaatg atcggcgtgt tgaccattgg tgaattggga agttcccacc gtaaatacac
    29641 agatgccgat gcccgcctgc tggaactgtt tgcaagccag gcggcggcgg caatttacaa
    29701 cgcccgtctc ttggaaaaca ccatccgcag gctggatgaa cttgaaacca ttgcccgtgt
    29761 ttcaggcaca ttgcgcgccg caaattctct ggaagagatg atacccgggt tgctggatga
    29821 gaccttgaaa gcattggaca ccaatgcagg cagtatctat ctgtatgacc ctgcaagcgg
    29881 aaccctgcaa tcttacattc aacggggatg gttcggggag aaaatgccgc attcgcagag
    29941 acccgctgaa ggtatcgcag gattcgttta ttccagcggt gaggtatatc atctaaaaga
    30001 atccatgcag gagtggaagg aaattccaaa tggatgggat gcggtctgta ttcccattca
    30061 aacttcgcag gaagtggtcg gtgtgttgtt tgtttcggtg caaatgccgc gagagttatc
    30121 ttctgaagaa gtgcgcctgc tgacaacgct ggctgagatc gcaggaaatg cgattcatcg
    30181 cacccgtttg catgagcaga ccaaacgaaa cctgactcac atctccatcc tgcggcagat
    30241 cgaccagacg atcgcttcca actttgacct gtctgtttcc atcaacatgt tgatcaacaa
    30301 tgccctgacg caattgcagg cggatgcggt cagcattttg cggttgcggc cagccactca
    30361 aatgctggag tactttgcag ggcatggttt caaaaatgaa ggaatgatca ggcaggtccg
    30421 cttgcggctg ggtgataaac acgccggaca agccgcgctg gaaagaagac aggtcagcat
    30481 taccaatctt tccaactcgg atcagttttt ttcaaactca gactttgccc gtttggaaaa
    30541 tatccgcgct tatcacgcca acccgctgat cgcaaaggga gaggtgaaag gtgttcttga
    30601 agttttccat cgtgagccct tccaccccga taaagaatgg ctggaactga tggatacgct
    30661 ggcagggcag gcggcactgg caattgacaa tgccgagtta ttcaacaacc tgcagcgctc
    30721 gaatcaggat ttgaagtttg cctacgatac caccatcgag ggctggtcac gggcattgga
    30781 tctgcgcgat cgggaaactg aagggcatac tcaacgcgtc accgaaatga ccatgctgct
    30841 ggccagttcc atgggcttgc aggcagatga actggtccat gtgcatcgcg gggctctact
    30901 gcacgacatc ggcaaaatgg gcgtgccaga ccaaattctg ctcaagcccg ggcgcttgac
    30961 gatcaaagaa tggaagatca tgcgtatgca tccggtctat gcctatgaaa tgctccaacc
    31021 gatcgcctat ttacgccctg cgcttgaaat tccgtatgcc caccatgaac gctgggacgg
    31081 cagtggatac ccccgtgcct tgaaaggtga ggaaattcca ttggccgccc gcattttctc
    31141 ggtggtggat gtttgggatg ccttgctttc caatcggcca tatcgccgcg cctggaagca
    31201 acaaagggtg gttgagtata tggaaaccta ttcagggaaa tattttgacc cgaaggtcgt
    31261 gaattcgttt ttgacattga tgagcaaata gacaggagtt catttcagtg aatatggatg
    31321 tattctccct tttggacacc gtccaaacca ttgcacgcaa cggcctgctg cacgtctcca
    31381 acgaatacga taaggaacgt tacgaacgcc tgctggaaat ggccgcaaag acatacagcg
    31441 atattctaaa tattccctca gagaagataa agcagggatt ttcaaatgaa cttggcacca
    31501 tcacgccaaa attagggacc aatgcagcga tcttcaacga acgcggcgag atcctcctca
    31561 tggaaagggt ggatggcagc ggttggtgcc tgcctgctgg attcgttgaa ccgaacgaat
    31621 caccagccga tggaattcta cgggaagtac gtgaggaaac agggttggag gttgagatca
    31681 aacaacttgt gggcgttttc acccgaaaac caagcgccaa aatggggccg catactgtgg
    31741 tgtcggtcgt tcatttgtgc gaaatcatcg gcggcgagtt gaagatttca catgaaggtc
    31801 atgccctgca atactgggca atggatgata tgaaaaactg gcatgcctcg catgaaatcc
    31861 atgcacgggc ggcatataaa atgtgggcgt cagagagatt gatccctgcc gtatcagaat
    31921 aaccataagc ccaattcgag cccgatgaac cgcttttcga tataatcggg gactatgcat
    31981 aaacacagtg ttgcagggtt atttgcaggg ttgggggccg cgtccatttg gggcgggatg
    32041 tatgttgtca gcaaggttgt tttggaggtc atccctccgt ttgcattgtt gagcattcgt
    32101 cttgtcatgg gggcgctggt attgggcatc atcatctatt tccgcaaaga caaggtcgtt
    32161 ttttcaaaag aacaattctg gaaaagtttt tttgtcggcg tggtagggta cggggtttca
    32221 ctcggttttc aatttgtggg cacgaaactt tccaccgcat ccaatggatc gttggtgaca
    32281 tcggcaacac ccgcttttgt tctgatcttt gcgccatttt tgctcggcga aaaaacgacc
    32341 acacgccgca ttgttgccct ggtcgtatcc acgctggggg tgttggcggt catagatccg
    32401 cgcaatgccg aactttcccc caccctgttc tggggcaata tgagtctgct tgcggcggcc
    32461 ctgacttggg cgttgtattc ggtgttggtg cgcaaggtga gtcaatcggg tgatctgctt
    32521 acctccagca cggtcatgtt gttgggcggt ctgccttcaa gcatcgcctt cggattgtgg
    32581 gaagtcaaca cacagggtgt tggtgcaatt acacctggca ttatcggcgg cttgttattt
    32641 ttggggatcg tctcaacggc aattgccatg ttcctgtgga attacgcctt cgcagaatta
    32701 cccgccgctg tggcatcgct gacgttcttt gcccagccgg tagtgggtac gctgctcgga
    32761 tggttctttc tcgccgaaaa aatcaccccg ctcttcctgt tcggcggagc tttgatcagt
    32821 gtgggcattc tcatcgcaac acgggagtaa gatcgttttt cggggagggg atgcaatttt
    32881 agcaggacca agtttcggtt aagaaaagaa gcggatgcag ttttgaaaaa ctgcatccgc
    32941 tttgttaatt cccaaagaaa aagagctatc gcttcacaac gttgctttcg gcaagtttgc
    33001 gcgcgaggtt gacagacgca tcgctgccga cggtgtgaag cagtgccatg ttaggcgggc
    33061 gctgcgcacc gacgggatat ccgcagacca acaccacctg ctgacctgag cgaatgccag
    33121 agttgagcag cacggcatcg acattggcga gcatttcatc catgctgttt gcgaattttg
    33181 tgaggttcgg ctgtacaccc cacaaaaagg aaagctggtt gaaggtttca tactcgggtg
    33241 taaatgcgag aatatttttc gaagggcggc cctttgacat aagccaggca gagcggccct
    33301 gcatggtgaa aacggaaacc gcgctgactt cggggtcggt ggcaagcgct ttggctgcgc
    33361 gggccatgga agccgcatca ctatcgccga ggcctgccct gccatcctga cgactgcccc
    33421 actctgtaaa atgcgactcg gcttcgctga cgatgcgcga catcatggaa acagcctcac
    33481 ttgggtattc acccgaggcg gtttcagcag aaagcatgac cgcatcagtg ccatcaaaaa
    33541 tggcgttggc tacatcagag gcttcagcgc gtgtgggcag cggattctgg atcatggatt
    33601 cgagcatctg cgtggcagtg atgaccagtt tggcgcgggc gtttgcagag cgaatgatgt
    33661 gcttttgcaa agagggcacg cgttcgggtg gaagttccac gccgaggtcg ccgcgggcaa
    33721 ccatcacacc atccaccaca tcaaggatct cttccagttc gttgagggct tcgggctttt
    33781 caagttttgc gatcagcaat ggggtgacaa acctgccctg cgaaaattcc ttgatggcag
    33841 cacgcaccga gcgaacatcg tcggcactgc ggacgaaaga aatggcaaca gcatcgacac
    33901 cctgcgagat gccaaaggca agatcggttt tgtctttttc ggtaaagcct gcaatacgaa
    33961 gtttgacccc aggcaggtta atgcccttgt gcgaggacaa tgtgcccccc accaaaaccc
    34021 tggcatgcac cacgcgtcct tcggcggaga tcacctgcaa agcaaggcgg ccatcgtcga
    34081 ggagaaggcg gtcaccttca tggacggagt caaagagttc gcggaaatca actgggagaa
    34141 gctggttttc agtttgcggg ggttcgtcgt tggtggcgta aagacagacc tgttcatcca
    34201 ctgcgagctg cagcggagcg gcaagcttgc caacgcggat cttgggtccc tgcaaatcct
    34261 gcagaatacc gatgggcatt ccgaggcgtt tggaaaccgc acgaatggcg gcgacgcgtt
    34321 tttgatgctg ttcgtgcgtt ccgtgggaaa aattaagacg ggcaatattc atacccgctt
    34381 tcatcaaggc ttcgagagcc tgctctgaat cagaggcagg gccgatggtg gcaacgatct
    34441 ttgctcttct cataacgttc acttgcttcc ttctataaat gtaataaagt tcaacctgct
    34501 ccatgtgcgg atggcaattg aataatacac ccccaaaacg acaaaaagat acgctgtctt
    34561 ccctcattga tgcagttgac gggcgctatg ataggtaaaa aagccgatga tgtcaaggca
    34621 taaaaaatgc cgcaggggag tgaatatccc tgcggcatga tcttccttca tggagaaaag
    34681 cttttacccg taatgcagtt ctttagcctg acgggtcaat tcctcgcgga attgcggatg
    34741 tgccacattg atgagggcct gtgcgcgctg acgaatggtc ttgccataca ggtcggcaat
    34801 gccgtgttcg gtcaccacaa agcggacatg gttgcggctg gtgaccacac ccgcgccagt
    34861 cttgagcatg gcggtgatct tgctcaactg tgtgccatca cgcagggtgg caacacttgg
    34921 aagggcaatg atcggcaccc cgccctttga gcgggaagca ccataaacaa aatccaactg
    34981 gccgcctaca ccactgtaga gtttcggccc aatgctatcg gcgcagatct gcccggtcag
    35041 gtccacttca atggcagagt tgaccgccac catgcggtcg ttttgggcga tgatgaaagg
    35101 gtcattcaca tattccgtgg ggtgcatttc caccatcggg ttatcgttga cccattcgta
    35161 cagccgtttg gtgccaagga taaatcccgc caccaccttg cccgggtgaa gggatttgcg
    35221 ggcattggtc aaaacacctg cattcacaag gtcaatgacg ccatctgaaa acaattcggt
    35281 gtggatgcca aggtctttct tatcaaaaag gaacttcaaa acagcatcag ggatcgagcc
    35341 aatgccaagc tgcatggtgg cgccatcggg aattaaggaa gcaacatggc cagcaatctt
    35401 ctgcacgata tccatgtcac cttcggcggt catgcgatgt tcggggatgg gataattgac
    35461 aggcacgata tgagtcaatc ggctgacgtg aatgaacgca tctcccaatg tacgcggcat
    35521 ctgttcgttg acttcggcaa tgatgatctt cgccgattca gctgctgatt tggtaagccc
    35581 aacttccaca cccaggctgc agaatccatg ttcatcgggg gtggatacgt ggataaccgc
    35641 aacgtccaat ggcaacacgc cgcgtttgaa caaaagcgga aattcagaaa gcaggactgg
    35701 cgtaaaatcg gcgcggcctt cctgaactgc tttacgaata tttgcgctga tgaacatcga
    35761 attcacgcgc aaatggcctt ccatttcagg gccgacgtaa tcggcagagc cgacggtcaa
    35821 tgcctggcag atctccacat cggtgaggtt gggggcatac tccaccagcg ccgccaaaat
    35881 cttttgaggc acagacacgt taccggtgag aaagacgcga ttccccgatt tgatggtttt
    35941 gacggcttcc tgtgccgtcg tcactcgtga ttgataaata ctggctgtag tcatattatt
    36001 catcctcctg ctgctgcgcg ctcaaacgca caaggttgag cagtttgccg tcgtgcgtgt
    36061 tgatcgcagg ttcaagggat ggattgctca aaatctcttt cgttccaagt gccgcaattt
    36121 cagtgatata gggtatggcg gaatttacaa aagcatgcgt ggccgtacga gccaccgcac
    36181 ctggcatgtt cggcacacaa tagtgcaaaa tgccttcttc gatgtacaca ggatgctcgt
    36241 gtgtggtcgg gcgcgatgtt tccacacagc cgccctcgtc aatgctgaca tccataatga
    36301 cggagcgtgg tttcatggag cgcaccattt cacgcgtgac caaaatcggc gcacgctgac
    36361 caggtgacag cacagccccc accaccacat cggcatacat gaccgagcgt tcaatattgc
    36421 gctttgtaga catctttgtg gcaatattcg ggaatcgatc ccagatgcgt tgaagagcaa
    36481 caaggttttg atcgatcacg gttacatgcg cacccatgcc gaggaacatt tgcgcggcag
    36541 atgtccccac cacacccgcc ccgaggatga caacttcggc tggcggcacg cctggcacac
    36601 cgcccatcaa aatgcccttg ccgccccaat tgttctgcaa cagacgagcg gcaatctgcg
    36661 ctgccattgc gccgccgatc tggctgaatg gacgcaagac aggcagagac ccatcaggtg
    36721 ccttaatttg ttcataggct acagacgtga tcttcttttc aagcaaaata tcgatcttgt
    36781 cctgctgggt tgaggcgagg tgcaaaaaac ctgccaggat cgactcaggc cgcagccaat
    36841 tcaactcctc ttgcatgggg cggctcacct tcaaaagtag atccgcacgg ccaaagactt
    36901 cctccttcga gaaaaccaga cgcgcaccag ctttttcata atccgcatcg ctgaagccag
    36961 ccatcacgcc cgcttcatgc tcaacgtaag cctggtgccc gttctgtgtc agaatctcaa
    37021 ccccggctgg ggagagaccc acacgatact caaaggttct gctttctttt ggaattccga
    37081 tatacatgta tgcctccgta aaaaacccta aaggtttttt atcaccttta gggtttcaag
    37141 attacttcat atttagcact tcacgaatgg agggaagcgc ccaatctatg gtttctttgt
    37201 cgatcacaag cggtggagca aagcggatga cgttatcgtg ggtttctttt gcaagaatac
    37261 ccttcgtttg cagggcttca caaaaacgac gtgcgccttt tgcctcaggc ttgagttcaa
    37321 caccgatcaa caagccccgt ccgcgcactt ctttgatgtg cggactggag atctcactca
    37381 actgttccac aaaatactcg cccaactgct gagaccgttc agccagtttc tcttcgcgga
    37441 tcacacgcaa ggctgcgcgg gaaaccgccg ccgccaatgg attgccgcca aatgtggaac
    37501 catgttcacc gggctggaac aacccaagga tgggtttatc agccagcacc gcagacacag
    37561 gataaaagcc gcctgcaagc gccttgccga tgatgacaat atcggggcgg acatcttcat
    37621 ggtgagaagc gaacagtttt ccagtgcggg caagacccgt ctgaatttca tctgcaacaa
    37681 agagaacgtt gttcttttta cagatttccg caacattctt caaatatcca gcaggcggaa
    37741 taatgacccc tgcttcgccc tggatcggtt caagcatcac agccgctgta tttggggtga
    37801 tggccttttc aatcgcatcc gcatcgccat aagtaacggt gacaaagccc ggggtgaagg
    37861 ggccaaaatc atcgcgatag gaaggctcgg tggagaatga aacaatggtc actgtgcgcc
    37921 cgtggaaatt acccgaagcc acgatgatct ccgcctgatg gcggggcacc tttttcactt
    37981 gataagccca tttgcgggca agttttacag ccgtttcaac cgcctcagcg cctgaattca
    38041 tcggcagcga catttcgtaa cccgtcattt cagagagttc tttgtaaagc aggggcaact
    38101 ggtcgttacg aaaagcacgc gaggtcaacg aaacacgttt ggcctgatcg agcaaagcct
    38161 tcaaaatttc agggtggaca tggccctgat taaccgccga gtatgcactt aaacaatcga
    38221 gatattttct gccatccaca tcgtaaaccc acacaccttc cgccttctcg atgaccacat
    38281 cgagcgggtg ataattgtgc gcgccgtact gttcttccat cttgataaag tcttgagtgt
    38341 tcataaaatt agtttcctct atttgataat tgaagattga aagttaaagg ttgggagttg
    38401 gagatcgaag taaaaatccc aacccataat tcctacgcta catcaaacaa acgcgccagg
    38461 atcgcctttt gcgcatgcag gcggttatgc gcctgcggga aaataaccga atgtttgccg
    38521 tcagccacat cgtcggtcaa ttcatggttg cggtgtgctg gcaggcagtg catcacgatc
    38581 acatccttgc tggcttcgcc gaccaatttt gcattcacct gatagggcgg gaagattttc
    38641 tcgcgcttgg ctgtttcttc ttcctgtccc atggatgtcc atgtatcggt gtaaatgacg
    38701 tgcgcgccct tcacagcttc atgcgggtcg cggacaaagg tcagcttgct cttggtcttc
    38761 ttcgcgatct tctgggcgat ttcaatagcc ttcgggttaa catcgtatcc ttcgggggtg
    38821 gccacggtca cattccaacc gagcagggct gagacatgca tgagcgaaac cgtcacattg
    38881 ttgccatcac ccacatatga tacattcaga cccctggatt tctttccaaa tttctcttga
    38941 atggtcagcg catcagccat gccctgacag ggatggttgt aatcgctcaa accgttcacc
    39001 actggaatat cagaccactt tgcaagctca agcacatgct cgtgatcgaa cacccgcgcc
    39061 ataagcccct gcacataccc ggagagcacc cgcgccacat ctgcaatgga ttcgcgcttg
    39121 ccaaggccga tctcactcgg tgaaagatac agcgcatcgc cgccgcagtg acgcatggcc
    39181 atatcaaaag aaacgcgcgt gcgcaggctc ggtttctgga aaatcattcc cagcaccttg
    39241 cctttgaaaa tgggtttatt tccttttttg aaatactgct tcttgagttt tgccgccaga
    39301 tcaagcatat cctgaatttc ttcagatgaa taatccgaaa tcgcgagaaa gtccttcata
    39361 tttactcctt gttgtgaaag tatatcttta tagtgggatt ctgccacgtt tcaagcatca
    39421 tactttgcgc gtgacatttc gccatagttt acatattaac gtcatgccat gggcgagctc
    39481 cgcaccttct tccaatatcg catcaaccca tctgctccca tgccgagagg gttcgccaat
    39541 gtgtaggcac gcagaacatc ctcacttaag ttcagcgagg cggcctgggc attcatccaa
    39601 actgtaaatt tttcgcgcag ctcgtccacg ggcgggtcgg cgggcatgat ctcttcaagc
    39661 catttctcgg cttcatcaag atttttttgc agttcattta agtgccagtt cacatcatcg
    39721 aagatcccaa aatgagttgg cgcaatgcgg gcaaatttca aagacttcaa atgcgaaatg
    39781 ctttcgcgcc attttgcaaa atgcaattca ggcggcggca tcggtgcgcg caaataagga
    39841 aagcccggaa tccgcacccc gcctacatca ccgctaaaac acaggtcttc aaataaatag
    39901 gaataatgat gttccgcatg gcctggggtg tggatgggaa gaaaacgcag ggctccgatg
    39961 gcgatctctt ccgcatcctt cgggaccgtg agttgattct gctcaacagg cagaaactca
    40021 ccccaaagtg tttccatcct atctccataa atacgggtgg cactggcgat caatttttca
    40081 gggttaagca tgtgcgccgc cccattgggg tggacaaaga cctgcgcacc ttgttgtgaa
    40141 agccaccccg cagccccggc gtgatccaaa tgaatgtgag tgagaaggac atgggtgatg
    40201 ttgcggggag ataaaccttc ttccgccaga ccagctttca gcgcggaaag agtcgagcct
    40261 ggtccgcttt cgatcaaaat gttcgcgtcg ccatgtctaa ttaaataaga cgcaatggct
    40321 tgcgtcttat tttgaaagtt caaatctatt gtgataatac gggtgtttgc catgtacgga
    40381 tatcctgtga aatgaattcg gttgaaaatt ccgagagggt ctcggaaagg ttggcgtcac
    40441 aagccacaac gcaaacgggg acacgtcttt cgcgcaatgc gcgtacaagg ccagcagtgc
    40501 ttccacgcct gccgccaaat gtttctccat caagaaggat cacaaccggg cgaagatacc
    40561 gccgttgaag gtcatccaca gccagcaata ggtcagggcg tgtggatggt gtgacaagga
    40621 tcgcgcttga cccctgcggc atttgcgctg actgggcaga gaccagtgcc gccagtgaca
    40681 gcttgccatt ggcttctaca aaggccagtg tctccagcac cttgccttcc tgacgttcac
    40741 tgcggtcggc gggatgaacg gtaaaggtgt ttcctgcgct ggcgtatcca accgcgcgat
    40801 gacgttcgat gaagtaatgc gtgagtgaag cagtgatggt gatggcatac tcaagtgtgg
    40861 aaggcggcag ttgaaacctg gggcgtttgc caaacatcat cgcttcgaca gagatttcat
    40921 cgaattgata ggccttttcg agatggacat ctctgtgcag gtcaaggaac aaccagatct
    40981 cggcttgcgg gtcttgttcg aactctttta ccatcaagtg gttcctacga atactggtgg
    41041 gccaatgaat gcgtttcatc gcatcgccat gcacatattc acgcacacca gccgcgtgcg
    41101 gtgtgacatc tggtgatttg cggcggatga cctgcccacc ggacaacaag ccaggcggga
    41161 acaggaacga tttgatctca aaaatcatgg ggagaacaac aagcgtttga gacgctggga
    41221 tttccttgct gacccgaaac aagccaaacg gatcacctgt ggagatgcgt gtggggccaa
    41281 gaggaaaacc gccgcgacgc gtaagccagg tgcgggcgag gtgtgtccgc ttttggcggc
    41341 caagaacaaa agtgaacagg cgtgatcctg aaacaaatgg gattggcgac tggttgtaga
    41401 cttcgatcca cggggcgatg acacggctgt tgttggccac ttcaaaatgc tcttcgaaga
    41461 tatcccccac attggcacgt tgcaaacggg aaaaccgctg caaccccatg ccactggcag
    41521 accagcgggt ccagacccaa cttccaacgc caagcaataa actcaaatag atgaaacgcg
    41581 aataaattgc ggctccagtg gtcaggaccc ctgcaatgcc gatcagcaat aaaagggcga
    41641 ggagaattcg cccagatctc atttggtttt cttccgtgta gatgtgattt cgggtgaaaa
    41701 ttctccgcca ggtacgggtg tggcctgcac gacttcctgc acaattcggt cggagctcag
    41761 gtcacgcaaa cgagccgcag ggctgacaat gatgcggtga cccagaacag gcacagccag
    41821 cgcctgaatg tcatctggaa ggacgtaatc tcgtccctgc atggcggcgc gagcgcgccc
    41881 ggcccgggca agggcaaggc ttccgcgcgg actggcgcca agatacacat cggtgctttg
    41941 gcgggtgcgg gtggtcaact ctacgatgta attctttaca gcctgtgaaa tgtaaatgtg
    42001 cttcacatcg tcaatggctt gtaaaatttc ttttgttttg acgaccgcct tcaatgtttc
    42061 caacggatgt tgaatctgtt ggtcgctcaa cacacgaagt tcatcagtca ttgaaggata
    42121 gcccagcctc agacgcagga gaaaacggtc taattgggct tcgggaagcg gaaaagtgcc
    42181 ttcgtactcg atcgggtttt gggtagccaa caccatgaac ggctcaggca aactgtgcgt
    42241 caccccatcc acagtaacct gatgttcttc cattgcctca agcaacgctg cctgcgtttt
    42301 cggcgtggcg cggttgatct catctgcaag gatgatctgc ccaataatgg gacctggtct
    42361 aaattcaaat ttattctttt gctggttgta aatggaaacc ccagtcacat cgcttggcag
    42421 catatcaggg gtgaattgga tgcggttgaa aatgcaatca agtgagcggg ccagaccgcg
    42481 cgccatcatc gtcttcccca caccgggcac atcttcgatc agcacgtgcc cctgacaaag
    42541 taacccaatt acgatcaact caaccgactg cctcttgcca ataatgacct tttccagatt
    42601 gccgatcacc tgctctgcaa aagccttaat gtctgccatg aataaaattt cctttccaga
    42661 cgagatgaaa agattctatc atgcggcttg tcatgttggc aaacctaccc cagtattgtt
    42721 tgtatagccc ctgtaaaaca ggccattgga cgcctgcaca ccgccaattt gccccatcca
    42781 tgaggcagac ggggcaaatc gtgtataatt tcggtacgcc tcagtagctc aatggataga
    42841 gcgcctgact tcggatcagt aggttgtggg ttcgactcct gcctggggcg ccttattcaa
    42901 tccgtagttg cccccgcagg agggatgata tgggttcgac tcctgcctgg ggcgccttat
    42961 ttcaatccgt acaaaaccat gaacaaatcc cttatgacgc gccttttcca tcgaatacag
    43021 aaaaaggtgc gttcattttt tgttctggac cagtgggaga ttctggttgc gcccgaaaca
    43081 gagtacacat ccctttcatg ggaaaagcta aaatccctta cgccacctaa agaccgcttt
    43141 tgggcagatc cttttgtatg ggttcaaaat ggcattcact atctcttcat tgaggaactg
    43201 cttttttcaa accaccgtgg aagaatcgcc tgtcttcaat tggataaggc gttgaatatt
    43261 gtgtcaaatc aggtcgtatt ggaaaggcct tatcatcttt cctatccatt cctgtttgaa
    43321 tacagcggcc agttatacat gattccagaa acagggaaaa acaacgcaat cgagctttac
    43381 cgctgtatcc aattccctga tcaatggcag ttcgaaaaga cattgataaa caacatccgt
    43441 gctgtggata ccaccctgct ggaatcagat ggtaaatggt ggcttttcac aaacatccat
    43501 gaagagggcg gctcaagttg ggacaccctg catctttatt attccgacaa tcccctctct
    43561 gaaaattgga ccgcccatcc caaaaacccg atcgtgaagg atattcattc tgcccgaccg
    43621 gcaggccgca tcttttcaca taaccgacag ttgatccgac catcgcaaaa ttgttctccc
    43681 cgatacgggt acgcagtcaa cttcaacgag atcatcaccc tcaatgaaac agaatatgct
    43741 gaaaaaacca gacatgcatt cacaccaccg tcaaagggaa caattcaagc aacacataca
    43801 tataacagcg ccgcaggaat aaccgtcatt gatgcgaaca ttcgcagaag aaagttttag
    43861 gataattcca aaatggaaga ttcacaaccc aacacacaat tgacccgaat gcttttatcc
    43921 cgtttggaac gcatttccgc agactcatac tgggcacatc gtgccagtgg tgtacggggg
    43981 gcattgctcc gcatgctgga aaaggccgag gcaggccgac ccgttcaacg gtctgaaatg
    44041 aagcgactgg tcgatctggg ttttatcatt ctggaaaatg cgagtctgga aaaaatcaag
    44101 tagcctgttt tgcacaaaag tgattaaaaa gcaaaagagc gttgacgtga gatcaacgct
    44161 ctttttaatt ctacttacac tgctatttct tatcttcttc ctgattggtt tgttcttcgc
    44221 cagccagaag tttccgcttt tcctgtctac gctttgcgat cgctgagatc gccacgtatc
    44281 ccaaaacgcc gaccactgta atggaggaga gcacaatggc gggaatcagc caaactggac
    44341 gttcgaacaa tggcgggcca ccagcaagga tttcgggaac tggcgtagtg ctgaccgcct
    44401 cagcccctgg aacaacatta tcaacaattg agaacggtga agatgcatta cctgtggcaa
    44461 cttgttttcc gttcgtcact gcaaaaacac ccgccagttg actttccagt tcgttcacca
    44521 acaggctgtc acctgcaagc aaaacaccgt tacggtcatt gccagaaatc acaagaatgg
    44581 acttttcagc gttgtatgga gacaataaaa gctcaaggta accaacgctt accccttcag
    44641 gaattcggta tacaacctgc atctgttttt cgctggcaag gttactggcc atatcgaacg
    44701 gcgcaggaag catgtcgttg aactcagaca ggaaaggagt ctcactggca agaccaagga
    44761 taatcatcga attatcatca tgaacctgct ggggcacctc atcagaaaag gctgcttcca
    44821 aatttgcgat caagggatta aattgctgac ccaggttata ggctaacttc cctgcgatca
    44881 cccaactcgc gacatccgat ttcgggaaaa tgaaagcaat atcaccaaga tcgctgtgtg
    44941 ttacaaaaag ttccgggaag aacttcaggt cttttagcag gggctcagca ttgattccag
    45001 gcagggttac cggcaagtga atgctcgact gattggaaac agtaaaccac gggtctgaaa
    45061 agcctgacgt atcgcaggaa ggcaaaatga tcatgctggc acgaatatca aggcgattct
    45121 caccaaaacg aagtgttcca ggcgggaatt taattcgcaa ggtagtcact tgctcagact
    45181 ctttgctgaa aggtgtactg gaaatcacct gcccattcag ttctacagag aatgaggaaa
    45241 caccataatc aagcagcccg gagtggtagt agaccaagtc cacataggca tccttggtta
    45301 atacttgttc cttgctgaca tagaagatgt attccttcga ttttaagccg attccagaca
    45361 gggtttcagt gacgtatccc agactttcga aggtgaagtc ttcaacaatt ggaagcgtgt
    45421 cagataaagc ctgcacattg gaaacaaaag caactgtcgg gtcactataa acgaagaccc
    45481 tgccagagct tacggcctga ccagctttca tcaccgcatc ccctgaattc ccactgacag
    45541 ccataatcac cttggtggga ttccacgggg aaagggccaa ttccacaata ccgtccgttt
    45601 cagaggcagg tgaaagattt acaaatttac catctgtaat gggaattttg aaattaacat
    45661 cggtcagaac agggaattta tccgcagtac ccacaaagat catgtgatag agtgtgggat
    45721 caaagccagc caattgactg accgtgatga gttgcatgtc atacaccctg ccgatcatgg
    45781 aaccaaaccc agccatgaca ttcaaagccg attgaagttc caaagcatca gggttatcag
    45841 ccacaacaac aagagtgctg tcagggataa aggaattttc aaggtagaac ggagaaggga
    45901 ggcgtgaaag attcagttcc gggcttgatt cttcaaagaa gaggtcaaaa aacgaagtgg
    45961 ggttgatgac aattctggca ttgacatcgt aatcacagct aaattgcgcc tcaagggaaa
    46021 tcgtaagctg gtgccttcca tcctgcaaga tcgaagtcag ggcagtatcg ggaatttcca
    46081 accgaacagt atggcttccc atcccttcga gaggggttga gccgataatc tgaccgttga
    46141 atgtaaagat caaattgccg ccatacggat tggcactatc gacaactttg ccaaggtcag
    46201 cgccgctcag gaaaacatca tagttgatct caatttctcc accagggatc aatttccagc
    46261 taaggggggt attgaaataa atattagtaa ttccatatgg gctgttcagg gttcgctcac
    46321 gatagcccaa gtccaaaaag ggaaccttgg cattgttgcc attgatctcg attgccgcat
    46381 atttttcggg ctgtacatcc acttctggtg cctttgcagc agcatgcagt ggaatagtca
    46441 acaaaatcac cagtattccc aaaatcagga acatcttatt tttcataaaa actccgttta
    46501 ccaaacatca gccgacatta tataaatcag ataagataac gatatgtatc agacgcagaa
    46561 cgaaccagca tccatgatta acggttagcc aaacggccta accttgtaca tgttaacctt
    46621 ttcttcagac tccatcgata attcaagcgc tttttcaacc tgagagatca atacgttgat
    46681 ggattcgccg ccttggcggt ccgcgaaacc aattctcggg tcaaggacaa tatcgatccc
    46741 gctttcccct tccagcgaaa tgggctgatt cagcaattca cgaatccgtt ccaaacgatt
    46801 catagccgaa acaccatctg tagaaggcaa cagaatgctg aactccaatt gagaccatct
    46861 tcccacaata tcattgccgc gcaattgata cttcaaagtt tcagtcacct tgcgcaaaat
    46921 ctggtttaca taagcctgtg gcagagagtc gtaaatctcc tgaataccca aaaggtgaat
    46981 cacgccaaac gtcaaaacgc ttgatggctg gttgatcacc tcttgacgca tccgtcgctc
    47041 aaaatacgcc cgtgtaaatg ccgtagattc aacatccagc atattgcgtt cacccagctt
    47101 gccaattgtc cccgtaatct gatccttgaa gatcgccaaa cctacgccca aaaccaaccc
    47161 caccatcagg gcaaggccag catcctgggc gggctgaggc tgatacgggt caccggacac
    47221 caacgccttg tcaagaaaat caatgtcata gatgacatag aggttcttca cataatcgat
    47281 cgcatgctga ccgatgctat tggccagcaa agcagccaca tccgggtctg ggcctttcac
    47341 gctaaagcga ataatatttg catcaggcac tacagtaaca gaagttgaat agcccctaaa
    47401 atcattcggg ctttcacgca acaactccat tgtgctttcg atgatctgat ggctgttgag
    47461 cacttcggca taagtggaaa taatggaacg cttatccaat gcctcaaggc tattaaccaa
    47521 gtctctgctt tccgtattct gcatgttggg gctcacaatg aaccgtgcca ccgcctcata
    47581 cattggagta gtaaagtaat atgaatacac aagagaaaca ttgatcgcaa ccagggttgt
    47641 aaccagaatg atccaccacc cacgctggat gattttaaaa taaaaacgaa tttccataac
    47701 gaactctcca ataagctaat tgtttagtgt tacaatttaa gaataaatta taacatagcc
    47761 tcaaacaggg gtactttacc catataatga ttgcaatgca cgcgcaagta atccattttt
    47821 cctaaaaact acatcacatc gcggagcaac atgttttccc tcgccttcat tcttgttatt
    47881 ttactcgtaa tcagccttgg cctattgctt tacatctatg tgggctaccc gctgatcatt
    47941 gttgtactct caaaatatcg ccgacaaccc atcgtaaaaa caggcaccct ccccaccgcc
    48001 acgattgtca tccctgctca taatgaagaa gcggttatca accaaaaact tgataacatc
    48061 ctgagcctcg attaccctcg tgagttactg cagatcatcg tctgcgatga tgcatcggat
    48121 gaccgcacag ccgagatcat ccgcacatat gcatccaaag ggattcaact aagtgaaggg
    48181 gccagagcag gcaaagtagg tgcgctaaat cgggcgcttg ggctggcaac ggggcagatc
    48241 tacgtcatca ccgatgccga caccatccta aatcaaaatt cactgcgcga attactcgta
    48301 aatttttccg acgaaaaagt tggctgcgtg gtggcacaaa ctcggatgat gtcttccaat
    48361 gaacaggaaa caagcgaatc tggtggattg tactggcgct acgaagcttt gatccgtcaa
    48421 aaggaaagcg atcttcactc aaccgtagct gctacaggcc atttaatggc tctgcgccgt
    48481 gagatcatgc aacccatccc atccaatgta attctggacg atttctatct ggccatgatg
    48541 accatcagtc agggttaccg tgtcatctca gaaccgaaat ctgttgtttg ggagcgccca
    48601 accaacacca tgggagacga catcaatcgc cgtcgccgcc taactgcagg gcgctatcaa
    48661 atggtgttca tgagcaggga atatcttcca aaattatcaa gcctgcttcg ttttcaagtg
    48721 atttctcata aattcctgcg gctggctatt ccgcacctga tgattctggc cttgctgaca
    48781 aatatccttc ttgtggcact tctccctgcc ttatcgcttt cgcctctggc aaatgccacg
    48841 ctcattgcac tggccattct gcaaggaata ttttatgggt tggcactttt gggagcggtt
    48901 ctttcaaaca ctcctctcaa aaaatcaaaa gtcttcaaaa ttttcatgct cccatatttc
    48961 ctatgcgcaa cgaattttgc aagcattgct ggcctgcttt ggtatttctc cggacaacac
    49021 actgttcttt ggcaacaggc aaagagacaa taaaaaatgg aaacctgtcg cccgcttatt
    49081 attgatctgg ccgttggtta tggtggaagc agttcacgtg tgcttggcat tttaagagcc
    49141 ctggccgatc agggagctgc tcttgcaggt ttagatcaag gtgcagtagt actcgcggcg
    49201 caaaaagaag gcttgcctgt ttacacagta ggcgcaaaga aaaccgaccc gcggatcgta
    49261 tcgcggcttg tccgcttaat tcgtgaaaaa gattttcagg tgctggacac acagaatatc
    49321 caatccaaat tctggggcag cctcgctgct ggcataacga acacctccct ggtctcgacc
    49381 atcaatagct ggtacgccag cgagcatgga caaagttcag gcaaagaccg cctcaagggg
    49441 cgcatgtata ccgcacttga acttttcacc aatcgcaatc tcgacctgta cataactgta
    49501 tctgaaaaag accgtcaggc gcttatgaaa tcaggcattc cctcccagtc aatagaatta
    49561 atctacaatg ccgtcagctt tgacgactct caaatttcca gcgaccccga tggcttgcgt
    49621 aaaaagttca acctcccccc acaatccttg atctgtaccg ccgtaggacg cctggttcca
    49681 gttaaaggat atgacctcct gatcgaggcg gtcaggcagg ttgctgctca aattccccaa
    49741 ctttattgtt tgatcgtggg tgaaggcgaa tgtatggaag aactctccaa tcagatcaaa
    49801 gatgcagggc ttcaagaccg cgtgctttta acaggatacc aagaccgcaa caacgtcatg
    49861 ccgatcttaa agtccagcga tatctttgca atgccatcac gctacgaagg gacgcccatt
    49921 gccctgctgg aagcagctgc catggggctg cccatcatag ccgctgccgc tgggggtatt
    49981 cccgaactgg ttacaaacca ggaacacgcc ttgttggtgg aacccaacaa ccctgccgca
    50041 ctggcggaag gcctgcgcaa attatgctca gacctgaagt ttgcccaaaa gctgggagac
    50101 aatgcccaac aaagggtgcg cacagtcttt aacatggata cccaaatcca aaacactttt
    50161 catgcctacc aaaaagcact ggacaggcat gatgcaaaaa gaaaaaaata acctcaaaat
    50221 aattattgca ggaagaaatt gatgtattca gataaaacca accccaggaa attttcgcga
    50281 tctggcttga tgaaagtttt taccgatgcc gcagcggcga caggtctcat cagaatcggg
    50341 aggcagtttt gggccaggtc gttgaccgta cttaattatc atcgcattga tgacccgcat
    50401 cgcaaagatt tcgattcctt tcagccaaac attagcgccc atcccgctga gtttgaccgc
    50461 caaatggaat atctctcccg ttggttcaac gtggtctccc tgcaagatgt agtgacgtgg
    50521 ctcactggcg gaaaacccct cccgccctat gcggctctga tcaccttcga tgatggctac
    50581 cttgataact acaccaatgc atatcctgtt ctgaagaaat ataaattccc agctgtcatt
    50641 tatttgacca gcggacatat cgatacaaac gagccgtttt attgggacct ggccgcctac
    50701 tgttttctaa acacaaaaac agatcatgtt ttatttcccg ataagaaaga actcttctgg
    50761 aaaaatccca ttgaacgctg gcagatcgcc aaaagttgga tctatgccat gaagcctcta
    50821 ccagaggaag aaaaacgcaa atgggtaaat caattgccag aacaacttgg ggtttccatt
    50881 cccgataatt ttttccgcaa tttaatgatg aattgggatc aagttcggga aatggcaaac
    50941 aacggaatcg attttggcgg tcataccgtc aaccacccca ttttgacccg catatcactt
    51001 gagcaggccc gctctgaaat tgaaacatcc aaagccaaga tcgaaaagga acttggtcgc
    51061 cccattctta gtttcgccta tcccaatggc atgcagacag attacaacac ggatattcaa
    51121 aaaattgtgg ctgacgcagg gtataaagtg gcgttcacgc tgcaaaacgg cccgactact
    51181 ttgcaggaag ttcagcaaag tccgttcaca atacggcggg tctttatttc acacgcccat
    51241 tccctgtcac agtacgccac gattacaagc agatttaatc gtcttcgaaa ttagtttgat
    51301 acccgtttcc ttgcctcaag caaacgggta tagacttcca cgatctgaac tgcaacagtt
    51361 ttccaactaa aacgagtctc agaaagatga cgggcgtatt gccccatttc atcgcccagc
    51421 ttcgggtcgt tgacaacggc cacgatcttt tcagccaatt cagcagggga attaggcgaa
    51481 acgataaatc cgcttttccc attttccaca acctcgggca acccacccac tgctgttgca
    51541 atgacaggtt tcccaaatgt ataagcagtt tgcaacgcac cactttgtgt gccgcttcga
    51601 tacggataca ccaccacaga tgccaatgcc attagtggct ggatctcatt aagcgggata
    51661 taccgagcat caaaaatcac ctggtctgca atgccgagat ttcgattttt ctcaagcatc
    51721 tcgctcatac taatatgttt ggtcggataa cctgcaatca ccagtttggc attacaggtc
    51781 ttacgcgcaa tcgcaaaagc atcaagcaag tcatccaatc ccttgcttgg agccagcaaa
    51841 ccaaagaaca gcaccacacg ctcgtctttt tgcaatccat aacgctgacg aagcacgtct
    51901 gccgaaacag cggggatatt caacaaccaa cccgaattac cgtgtggaac agataaagtt
    51961 cgttcagcgg ggatggtgga atgcagggat agaaatctct tgcgaatctc ttccgaaagg
    52021 aagaagatgg ctgaaaaatg acgatagact tcgccgctga tacgatccaa aatcgaccag
    52081 ataaagtcct gactttcacg caactcaaat tcatgacaca actgagtgac gatcaaacca
    52141 cggcggcgca aatagccgac aaaataagac tcaaaggaat attcgtattt tgaaaactga
    52201 acgatatcag gtttttcgcg caacaaaaac tgggtcaagc tggcccaggc atagatcaaa
    52261 cggacaccgc gggccattcg tcgcagggtc cagtaaattt tccgccatgc acgtttcagc
    52321 ggattctgga tctcggcctt catcgaatcg gggtcaaagt gttcccaaag attgagcaac
    52381 ttctccacat gaaaactgtg gggttggtct gccatctcat attttgcccc agcaaccaaa
    52441 gtgacgtcag caccttcact ggccaatgca ttgcacaact ggtaggcaaa atggacaaga
    52501 ccgccaccgt tattcggttc aacgacaaaa atgcgcagtt tatttttcat attcttttat
    52561 caaacccaga taaaggtctc tcaatcgctc cacttgaaca tggatgttaa atttctcatt
    52621 gacaatattc ctgccacgtt cccccatggc cttacggcgg ccctcgtcag ccattagcga
    52681 ggcactggca gatatcaggc cgtcaacatc tcctgcagac aaaagcaacc cgttctcgcc
    52741 ttcatttatc atttcgggca caccgcctac actgctggca atgatcggca aacttgcacc
    52801 catggcttca gccaataccg tgggaagggc ttccgtcagt gtcggtaaaa caaagacatc
    52861 actggcagcc agcaagtgcg gaatatcgct gcgctgacct gcaaatacca catggtcttt
    52921 catgccagcc tgttcaactt ctttttcgag ggcttcgcga tacgttccat taccaaccac
    52981 cagataataa acatcggggt ctttggcaac cacagcaggc aaagcgcgga tcatatattg
    53041 aatccctttg aattcgcgca acactgccac tgtgatgagg attttcgcct gagcaggcag
    53101 gccaaattct tcgcggatct ttacacgatc ctgctgatgg ttcaggtgtt caaaatggct
    53161 taagtcaatg ccattgtaga tggtcagcag gttttgaggc aggagattgc tgatctttag
    53221 ataaaatttt cgggcttcct ctgaaacaga aatgaccttg ttgcagaaat ggcgcagggc
    53281 taaaagttcc acagtctggt gaagcttcga tttgagcttc atatcctgcg aaggcattgt
    53341 atgcatggtg gagacgctcg gcaggcgcat ggattttgcg gcaagattcc ccatgatatc
    53401 ggccatttcc aattgtgtat gaacaagatc ggcttttaca tccctcaaat attggcgcag
    53461 acggggaatg gctgtaacat cccgtaaata gggaatcagc aacaagtcca cgggcacccc
    53521 caaagcgcgc aggtcttccg cgatcgggtt gccatccttc acatgcagca cacatacgcg
    53581 gggttcaaaa tattccctgc tcagattttt caaaattgga accatcaaac gttccgcccc
    53641 ccccatgccc agaccgtcga tcacaaagat gatattgtat ttgcgtttcg cggaaatgtt
    53701 agctgccatc cagaaatcca tcttttggga tattgaccag ttcggtcaaa agattacgcc
    53761 agatcaaatc atcaaattga ttataaccaa aatcacgtat ggaccgcact gccttttcaa
    53821 tacagcatac ttgcattcca aggcggggcg gaagcagtga gtaacgctcc gtgggtccat
    53881 aggcagccag atatgcaggg aaaatctcct gttgccagtt gacaaggagt tttgccgtca
    53941 ggcttgcccg ctcttcaggt gaaccccgcg aagccgacaa agctcccgcc atcagaaaca
    54001 tgtacacatc cgcgctgaca tcactcgacc agcgtgaatg ctgccagtca acaaatatca
    54061 gcgtgccatg ttgtgaacta cggatgatat tgccgtgcca aaaatcacca tgaagcaagg
    54121 tttgataatg gactgagtgg cgcgcaaaat actgctccaa ctcagaaaaa gcaaatgatt
    54181 cttctgctgt gggagtgtaa atcttcttga aaagctcaag gttttcaagg aaatccgtcg
    54241 ggatacttac aacatccgca gcttctttgg ccgtcttttc gtgcaattct cgcagagcac
    54301 ccgccaccgt tttagataag ttcagcacct gaatgggatc atgccataat ttctttttcg
    54361 aagaaaacaa aagcccatcg ccgcgaacga aagagatcac agaaacctgc tgctgaccga
    54421 catcaaaaaa tgcgagcggt tcaggtacaa gcagagaggc gtctttaccc aaaagccccc
    54481 aaacctccgc cagccgatcg tattcctctc gaagaatgcg cccattagaa ggcaagcgtg
    54541 gaactttggc aacaaacgaa ggcacggtcg cttttccttc gaaaccaaag aaaatcacat
    54601 tgtcattcgg gtcggctgct ccgtttgaaa gcatcaccca attcagattg ctgttaatcg
    54661 aacgacggct gttcgaaaca agcagggttg tgagatcgtc aatcaatgcc gccatcacgc
    54721 ctccacactg gcaaccgcat agtagaacgg aacaaaaccc agcagcatgt tcacagcagg
    54781 cgttctggtc aacttcaaaa aataaccagg caatttatgc tggtatctgt aattcaaaac
    54841 gaaagcaagt gattgtgaat tcaaaggaaa gatatattca ggggtcaacg gctcagggaa
    54901 agtcccataa aaacgcattc catcaaaacc agccctttcc aaactgcggg atattctcca
    54961 ggtagtggaa gaattgggaa attcccgacc cttgcggcgg atatcccagc gattggagaa
    55021 tcccagcaaa aacatgccac ctgctcgaat caaactccga accgctaaca ggttcttccc
    55081 caactggctt tgcctccccg cacgattatc cccgccaaaa cccaaaggcg ccgcaacaat
    55141 atcaaagctc cccgattcaa attgaagggg ggagcgatcc gtgttgaagt tgtcgaaaga
    55201 aacagtttcg aagggaattc caaattcctg aaaagagcgg tcgtagtcgg catccgatgg
    55261 atgaatcacc aacatgcggg tgtgttctcg caatggaagc agaaaacgcc aatcaacccc
    55321 atgactgggg agtgaaatgt gcgcggcggt actcggggaa ctttgagaat tcatgcctat
    55381 ttggcgagcg cgtctgcccg ctctacatcg agcaggctgt agccgaatcg gttgaggcgg
    55441 gtctcaaaac ggctgatcat ccgacgggca acgaaccccg aaaggaaacc tttctggtct
    55501 ttctcccaca tggaaaagta ctttttattc acactggata agaccttctg gttattcggg
    55561 aaggggctca gaccaaggaa ttcataaatc ttattggtgt aagtggaagg gtctgccaca
    55621 aactgctcgt aattcaggag catggcattc ttcaaatgct ttttgtcttc caaaaagatc
    55681 tcatggcaaa ccagccagtg ctcaaagatt cgcggcaggc ggcgccagta gatgcggtat
    55741 tttttgtacc aggcacgggt ggcataagaa accgccagtg gatgacgcat catgacgatg
    55801 aaatatgagt cagggaacat cgcttgcaaa aaacgggtgc gaatcagatt cgggggggat
    55861 ttttccagca agtattgttt gctcatatcc caatacgggg tccattcggc aaacaatttt
    55921 ttgcggtttt cttcggttac caacggagag gcttcggtga ggtgcgcttc gggattgaaa
    55981 ccaaattttc cagcgccgcc ataatacccc gacggtttgt ataccgtctg aagatgcatg
    56041 ccttcatctt cgggagagtt cgtgttatgg aagccgctca cttcgggatg gtcacgcagt
    56101 gacttgaaca ggatggaggt accgctgcga tgcaatccgc tgacaaaaat aaaacgatga
    56161 ttttgcattt tagatttcct ctacgctttc ttctttgaac caccgatcgc cttcatcagg
    56221 cgaaagagat tctctttttc ggtccaccat gccacgccaa ggtagctgac ggccccggtg
    56281 atcacaacca atatcagttg aacaatggga gtaagccccg aagcagcata aagaactgca
    56341 aatgccagag gggtcattac caagccagcc cgcaaggatg gcaccaattg actgaagatg
    56401 tctttgaggg tcacattcac aaaccgcgta gcaactgcca ggctgaccac tctgcgaatt
    56461 agaacagcga tcaaatgtcc ccaggcaatg ccgatcacgc caaactgggc gccgatcaac
    56521 aaggcaggga ccaaaatgat caaggtgaaa acagagagat tgaaaagaat gtaaggccgc
    56581 ccgaccgcct tgtaaatacc gccaatatga aaaccaatgg aatacaccca ggcataaatg
    56641 gccagaaccc tcagcatcgg aatggcttcc aaccactgtt caccaaacac gaccctgata
    56701 atcgggtcag cagcgataaa gagaccaagg gagatcgggg tggcgatcaa ttccaccaga
    56761 cgcacagagc caagaaagcc gttgcgcatt tcttcaggct tgtcctgaat ggaagagaaa
    56821 gctggaaagg cgatctcacc cagaacccat aagtttccga tcaaaagcat ttcgggcaaa
    56881 cggtatgcaa gggtgtacac actgagttgg gcaagcccaa acaagcgccc tacaatgatg
    56941 taatcaatgt tatcaatgat cgttccaagg atgtctccgc caatgatgga cgacccaaat
    57001 ttgatcaaag accccgcaat gcttcgatcg atcatcaaac ggggccgcca cggcaaaatg
    57061 atccaaacca ggatcaccga agtcaatgcc ccggcaatct ggccgatcac catggcccaa
    57121 acgccaaatc cagcgaacgc cagtccgata gaagcaattc ctttgacaac tgtattgccc
    57181 atatctggaa tgaactttcg gcggtaatcc aagtcacgca acagccagat catatgaacc
    57241 gacccaagcg cattgatcgc aaaggaaagc ccaagccatc gtaatacggg cactaccccg
    57301 gggtcacgaa aataatcagc aatgggtggc gcaaggggaa ttatcaaagc cgagataaaa
    57361 aagccaagca tggtatttat cgtaaatacc gtattggcgg cattatccac ttcaccgcgg
    57421 cgttgaatta aggctacgcc caatccaagg tctttgatca cagccagata attaattgcc
    57481 accacggcaa ccgccacctg accgaattca gcttttgtca aaagacgtgc caaaacagat
    57541 gtggttaaca gcacaacgag tttgccaaac ccaaaagtga gggaattcca aagaagccca
    57601 cccgccactt tgcgcgagaa ctggctgcca gcatcttgtt ttacagattc atcagtcatt
    57661 ataaagtctc aataagaatg gaataaaatc acgaactata gggatattat tgaaaacatc
    57721 ttgataattt actcatccat gcaggaagca aattgtgaat ttttcaagca ctgagaaacc
    57781 tcatcgtaat taacgatcag ctgttccagt ttttccgtgt tttgcttcat tatctttatc
    57841 ttggtaaagt ctatgtcatt tacgcccaaa aagtcttcag cctgcttaaa taccaccttg
    57901 ggaattcgaa ccagctcttc gtaaaccacc tcaagcatgg gacggccagc aaatcgcttg
    57961 cgggccaact cctcctgggc ttgaatcttc tccagtcggc cgatcagttt ggaggtgtca
    58021 agcgtgatcc gttttttatc cagttgattc cccttggagg gagaagagcg tgtccattca
    58081 tctgttttga aggcaatatc cagggaaacg attgtacgga ggcgatttgc acgggttaag
    58141 tgaataatcc taaattcctc atgttgcaga aatttagccc actcttcgtc ggtcaggtgg
    58201 ttgtaaaaaa gtttgaagcc caccagctgt gtatccttgc tttccttgcg gtatacctgc
    58261 gagagcactt tctcaaaatc tttaccaatg gcacggttgt gatcagcaaa aatttcatga
    58321 tagccggtga tggatttcga gcttatcagg ccatcaaaaa gataatttga acctgttctc
    58381 gcgtggccca aaatgacaaa cctatcattt atctgttttc caaacaaatt atgataactg
    58441 cgaaccagaa aattttcata aaccaaagca ggcaatgaac gtaattttga catcattctt
    58501 tttgcatcca tataaaacca tcctttaaaa atagcaatac cgccaggctt tataactggc
    58561 agtctttttc agcgccgaaa gttgccgccc tgcctcattt tattgccaat gtaacaaagc
    58621 ggcttggtat ttaattataa atctagacgg taaaaattaa actgtcgttt tggaaagggc
    58681 ggcctgattg gtttccgcaa ccatctcatc tgtcaaccgt attgcggtaa gcgacaaccc
    58741 aaccaagatc caaaaatatc gaatatatgc cccatgcagg aatgtggcag caaccaaata
    58801 cccaaccatg gaagtttgca gggaactgat ccatggcacc cacgactctc ttaatgcagg
    58861 caaataatca atggagcgtt tcaccttatt taaaccacta aacactgaaa caatcaccgc
    58921 cacaaaagcc agaaacccca atattccatt ctcagccaaa agttgaacat aaagggaatg
    58981 tgggtcacgg gcttcagcac gttgctcaat accgatcagt tgagaatatt tctggtaatt
    59041 atatttgtaa ttacctgcac ccacccccaa aaatggactt gccttaaaca tacttaaccc
    59101 ggtcaaaagt tcactactac gacctctaaa agagtcttcc tgataaatgc cgctttgatc
    59161 tcctgaggtt aagatgctta gtgaagcaaa gcgctccgta tagcttgaag gaataaacgg
    59221 aattaacaat atgatcaatc caaccccggc gaatgctatc atgggactga acttatcctc
    59281 aaacacaaaa aatatcaaaa caacaacaac ggccaacgcg aggtaatctc cccggctgta
    59341 ggtgttgagt acctcaaaga gaaaaacacc aagaaccaac atggagaata atcgtgttgt
    59401 catccgcctt tcgtgaataa tacgaaaagc aacaagggcg atcacagaga ccacagtttg
    59461 gccccacata ttgggggcat taatcggccc acccaatcgg gctgcagttg agccggaaaa
    59521 gtcatcaagg cttaccatgg caaagccaaa aaaattattg ctgtagttgc cggtgatgga
    59581 ttggtagacc cccaaaaggc acaacatgaa cgttactaca atcaccaccc acacggctcg
    59641 cttcaaggcc tcaggggatc ttagtgtgta cattaagcaa taaataacga caatatcctt
    59701 ggctacgtca aaaatcaccg aggatgcacg cgatttgtct gcagccatca tgaaagaaaa
    59761 cgcataaacc aaaaaataaa gaaaaagaaa tgcctcaatt ctcgtggttg tcgaacgggc
    59821 agcggggggt ttctcaaaat aaatgaagcg cgcgacgatc gtagccacca caaccactac
    59881 aagaggcttg atgataccgg ggtagccgtt gtttgacaat tcatcggaca catttgaaaa
    59941 cacggcaatg atcaggacat ttgcgccgaa gcttggcatc acaaacatgg tcaaagccac
    60001 agcaataccc accaatccca ccaaaccata taagccaatg tcactgtcga ggttgccaat
    60061 ggcaagagca ctccctgcag ccaaaacaat gcttaacaca aaaaacacga catcactttt
    60121 ctctattttt ttaaggcggg taatcatatg catatcctct aaaaatcaac ttgactactt
    60181 tatggttgtg gacgggtagt tccaatccaa aatatcatac cattccgtgg gaatattgcg
    60241 taacatatca tcagaccata tttcaggatc ccaatttgcc gtagcctctc cttcaggata
    60301 ggtcttcgga tgattgctat tatcaacaat aaaaccgcca tatttctgca tggcaagcac
    60361 ccaaacgata caacccttca tacccgaaca tgcattatcg atttcttctt tggttatttc
    60421 cgggtgaatg atcatgcgcg caccttcggg tatgtcatac gtggatgtgc ctctaccgtc
    60481 ggttttcgta aaaggcggga tgaaagcagg ccgcccattg gcaccacacg tctcaggtgt
    60541 acatggaaaa tcataagcca gtgttacagc atgctcaatg acacccaaat caacttcaca
    60601 ttttcgtaca attccagaaa aattcccgat ccctgcccca cgctgaccaa agccttccgg
    60661 gggaactcca gaattctcaa tactataacg gtatattccg ttggcaaacc aacttcccaa
    60721 gggatcaatt gtccccttgt ttaaacccca ctcctcacca gtatctacat tgataaccac
    60781 catttgccca tcagttcctg gagcaggttg tgccccctct ggaataggca tccacacaga
    60841 ggcggcgggt tcaccatact ggatttcttt atcatcaaac gcatcacgaa agcggttctt
    60901 tcgaagtttt acaggcacca gtggcgtttt attatcaacg aaatagatat taggagcata
    60961 ttgtgttggg tcagaaccga tccatcggct tccatcccaa aatgcctcca tcattttgtc
    61021 gctgtcagga tgaattttcg caatagacca gtcaattgga atattccaaa tggaatcttt
    61081 ggtataggga atgtcacagt ctacgtcggc atattcggaa gagttgggat gcgaaaccac
    61141 atcaccccca ttatcaaagt ccccccccag ttcagatgaa acgggggtgg attctgaacc
    61201 atgagtacaa ctcgtaaata atagacaagc caagataata aaaaaaggaa ttcttttcat
    61261 agtgagtaga gtagaacaat atcagcttga tgtggaaaga atgagattga aattataccc
    61321 tgatacaggg tagtcttttt tattatataa aagctgactc gaaaaagaaa tttttagaac
    61381 acatcatata gcaaaagcgg caagctcgcc tctgaatagg taggcaaaac ctgccgctcg
    61441 aaagcgtctc tttttttcaa ttctgtgcta atgtgccccg cgcgctttga aaacggcaaa
    61501 aactgtaaaa aacaggatat taatatccat ccaaatgctt cgtctttcaa tataggcaat
    61561 atccaggcgc agacgatcat caaattccat cgaagcacgg ccaatgatct gccaaagccc
    61621 cgtcagacct ggggttacat ccaacctttc ggtatgccac aacttgtagg tttcagcgcc
    61681 aaaggaagtc gggcgcggac caaccaaact catatcacct ttcaacacgt tgatcaattg
    61741 tggcaattca tcaagacttg tttttcggag aatttttccg atcttcgtaa tgcggggatc
    61801 attggtgatc ttgaaatctg gccactgcaa ttcattcagg tgagcgtact tttcctttaa
    61861 agcctcagca ttcggaacca ttgaacggaa tttatacata ctaaaacgtt tgccattttt
    61921 tccagttcgg aattgcgtga ataacgctgg cgccccaggc gaggtaacct tgataataat
    61981 tccaagaacc aataataaag gagcccaaaa tggggcgctc aagagcacca gagatagatc
    62041 cattatgcgt tttaaaataa aataagaacg ccctacaaaa atgcgtttct ttgggtcaaa
    62101 agaagcaatc caggaatact ggtcggcagg gttgatggat gtgctgctca tttaacaacc
    62161 tgctctttca tttcaatggc tgccttggac ggtaaaggct gtttggaacg atcacgcgcc
    62221 agatgaagca aatcttcaaa agtcagagct tcatcgggaa aagaggccac accacaattt
    62281 acatgaagcc ccgtccgctc cacaacaatt ttggaaagac ggtctgccag taggtacaca
    62341 ctctccaaat ctgtttcagg gcataaaaca atatacctgc ctacctggtc gcggacaaga
    62401 aggtctgtct ggcggatgga ttcaccaatt gcctgaccga ctcgtgcatt ggacaggcgg
    62461 gtaagcacat cgcgctgcaa actcattaac atctcacgaa caactgcttc atcttgtgga
    62521 aaagcatgca agacaagcag cgagagtggt cggtgatatc gacggcttcg gctgaactca
    62581 accttgatct gctcgtttgc aaggtcaatt tcaagtgcgc ggtgagggaa tgtcccttgt
    62641 gccagaacat ccatcagcga ttccgagcgt tcaatcccaa gtgcgagttg gaaggagagc
    62701 caaacgccca cttctatgat gaccagttcg aggacaacaa cctccacatc caacgacccg
    62761 gtattgccgc gatcagtaag ctggaaaatt ccaaaataga ctgcgcccca aaaaaccatt
    62821 ggcacaacca ccgaaatttt gtggagcgac ggcacaaaga ccatcactgg aacaataaaa
    62881 attgccagca aataaaaata ggatgcaaag ttaataatcg gtgtatcgga gcggtctaac
    62941 tgacccagca ccaatatacc gaacaggaaa aatgcgatcg acaaaacaga tcttttgagg
    63001 tttgtcacgt gattacccta aaaaaatatc aatttcatat taaataattc attacttgat
    63061 tttagcataa aaaggctatt aatcaattcg aaaaagtggg gacggggaaa ttccacagct
    63121 tgagagacca tgcccgaagc gcaacatctc attttcgatt tggataaatt cagcccaaat
    63181 tttacccttt atattgattt gtaaacatga cggtttcgtt aagtggtaag aatcttttcc
    63241 tcttgcacat caaacgcatt gaacaaattc atcctggagg aaatttttct gtgagcaacg
    63301 atctttacaa taaaaaccca tctcaaatcg tcatgtattc caccgagtat tgtccagatt
    63361 gccaccgggc aaaaaagttt ttcgaaacaa atggtattga gtacttgaag gttgggttgg
    63421 agaacaacga agaggccacc cttttcgtga tggacatcaa cgatggcaat cgaagcgttc
    63481 caactattat ctttcctgac ggttccgtgc tggtcgaacc aagttgggaa gagttacgga
    63541 taaagaccgc catgccaaca gaaaccctat agttcgattt tttctcttca gcgccgacaa
    63601 aagtggaatt gcttttggcg gcgcttttgg ttatatgctg aacacgggag tataatgatc
    63661 agaatggtga agagaatatt cacgctcctg tttttggtgg tgatacttag ctcctgtgat
    63721 acttggggga acctgccgca gccgctcccc gtgtggaccc ccgttccttc tcaactgcca
    63781 aacatcatta cagccacacc catcatccta accccggggg tgatcgttct aacccctgtt
    63841 acaagcactg ctacaaacac tgaaacccca tcccccgtcc ccacttttac atttactcca
    63901 ttcacgccca ctgaaacccc tgtaacaccc atccaagcct tgacagtgac catccttggc
    63961 tgcaacacca gcattgacat ccttcacggc atgggcgaag taacgaatgc ctatgtaacc
    64021 gtgaccaata caggaacaac ggacctgccc aacacctgcg ctttgctgcg ggccattgat
    64081 gaagaccgcg accatcctga taaaaaagtt tgtgttccca acctgcccat acagaatcaa
    64141 gtgacactca aactgaccgt tgactcagcc tataacaaag acaccattat ccaggtcgat
    64201 gcttcatcca acgatttgtt gttactgcgt gtcgataggc aatcctgtac ggatatcagt
    64261 ctttttggcg gcaccccgtc agatatcggt gtagtgaaac ccattcagta aactcgatct
    64321 ttacttagcc agaggcagtg taaacgaaaa gacactgcct ttattttttc ctgcactctc
    64381 cacccagatt cggccgccat gcgactcgac aagatgcctg gcaatggtca aaccgatgcc
    64441 agaaccacca tgcgcacggg agcgtgattt atctacccga tagaaacgat caaagatgtg
    64501 tgcaaggtgt tcagcaggaa tacctgtccc agtatcgtaa accgaaaatt gcgcttcgtc
    64561 tttctctttc ttcacggaaa tggtcacact tccattttct ggggtgtatt gcagggcatt
    64621 gcccgccaaa ttagtgagta cctgaatggc gcgcccttcg tcggcaagaa catgaatggg
    64681 gtcagctggc aggttgaggg tgagattgat tcgtttttca tcgtattgat attgcaaccg
    64741 tttggcgatc gtttggatca gaacagtcgc atctacggcg cgaaggtcca attgaatggc
    64801 acgagattct acgcggctca actcttgcag gtcgtcaacc aaacgactca agcgttctgc
    64861 ttctgcatgg atttgttgat aggtttcatc agaagcagga agtatgccat ccatcagggc
    64921 ttcagcagag cctttgatgg cggtaagcgg tgtgcgcagt tcgtgggcga catcgcccaa
    64981 taaacggcgg cgcatgtttt caacctgctc taactgttct gccattttgt taaaactgcc
    65041 cgcaagttga ttcaattcat cggtgccttg cagttgcacc cgttcatcat agcggccttc
    65101 agcaatgcgc tgactggcgg tcatcatcac atacacaggg gaaaggatgc tgcggctaaa
    65161 gacaaagctg acaaccaatg caaccagcga agccgcggca acggcaatca gcaacgcctc
    65221 ggtaaaggtt gcctgaaaac tgcgatagaa ctccgacatc attccaccgc cctgaccttg
    65281 accctggccc atgccctgcc ccatcccttg tcccattccc tgccccattt caatctgatt
    65341 ttccattgcc aaaagatgac gcgcaaaagc gcctggtgta acgatttttg tagtaattcc
    65401 aatgacggtc atccccacca aaatgaccac gaaataagaa agaaataatt taattccaag
    65461 ataagatcgc agaattttca tagcctgctc aattctttat gccgtttcat cgtcgaagcg
    65521 atatccaata ccacgcactg tgctgatcac atcgggcaag ccaagtttct ggcgcacgtg
    65581 accaagatgc acatccacaa cacggagttc accgaaatat gaaccacccc acactcgttc
    65641 cagcaattgc tcacgagtga ggacacgtcc acggttctca gcgagggcat gcagcaggtc
    65701 gaattcgctg gcggtcaaat caatggtctg atcgtccact gtaacttgac gcgcacctgt
    65761 atcaatgcgc acatgacgaa acgccagcac gcttccttcc gcgctggggc ctgttcctgt
    65821 ttgaatgcgc ctgatcgccg ccttgaccct tgccaccaac tcccgcggac taaacggctt
    65881 ggtgacatag tcatctgcgc ccatcgaaag acccacaatt ttgtccgtct cttcagtgcg
    65941 ggccgttagc aaaatcacat acgcctgcga ctcacgccgc aagcgggaaa gtacttccaa
    66001 gccatccata ccaggcagca tcacatccaa cacgatcagg tcgggtttaa aagcacgcgc
    66061 cgccttgagc ccggagggac catccaccgc agtgaagact tcatatcctt cgggtttgag
    66121 atacgcttgc accaagttca caatggacgg ttcgtcgtca acgataagta tctttgtcat
    66181 gatgctattc tctcgcggtc atttttattc atcataaagg cattgtaaag atttggtaaa
    66241 gaaaaagcca cagtgaacaa ggtctttcac tgtggctttt tacaaaaatt ttcctatttc
    66301 gattggcaat accgctgaat atgctcatct acttgagcct gaagctgtag cgcgcgctca
    66361 gatgtaggcg caaccacgat acacccaaat cgatttttga ataatggttt ggtaatggtg
    66421 actaccattc ccagcggctc gtctccgggc atcgggtaga gaaaatcctt gattacctcg
    66481 agcaattcca tggcatctgt aatcgtacca gagttggaag cctcaaaact gatgagagag
    66541 accttactga cataatcatc accaaaagta tattttccaa attcatgcac atgtgtgccc
    66601 ccagtgcggc gggcattgat ctcgagcaaa tacacttcat tgtcgtcgct tacgatgcaa
    66661 tccaggtcaa aatgaccgac atatcccata ttttgcaggt tatgtgccaa ttcaagggtg
    66721 caacgtttca agtctgcgca ccagggttcg ctataaatgc tgcgatcaat ctgaataccg
    66781 caaaaatccc caaatcctaa aaacaactga ttggatatat aggtaatttg aggttcaccc
    66841 tccccaagtt tgggcacctt tacttcagcc gagggagata ttcgatttgc cgaggggata
    66901 tatttctcaa caacgatcaa ttcgttggaa tagaaaggtt gggaatgcag cttttcaaaa
    66961 ataccctcaa cagataaatg gtcttcagga gaaatgaccg tcgtaccgat accgctttcc
    67021 ccagtatcgg tctttaccac gcaagcttct ccacggctgc aaaaccaatg cgcggcaatt
    67081 gcggcgtgct tcaaagtttc gcaaatcatg ccaaatggca ggaatctact ggcgtccgtc
    67141 atccaaaaag aggccagagt acgaaagccg gttttggtgt cgatataatc acgcagccaa
    67201 aaatgatcag catcagggct ttcaggggtt aatacctgca agtggtgttc cctgcgcaaa
    67261 acttccacca actgaagaaa ttccttcgtg gtggcatatg ggataagttg aacaattcca
    67321 tttggggccg catactgcag gataccttca agaagatgtg cttcacgtaa aatatcaagg
    67381 cacagataat gcgaaggttc atgaggagcc agatggctcg tacgtgaata gcccagccgt
    67441 tttacgttga actctgcatg ggcgatcgga taattgacaa ttaccaactt tgggtcatca
    67501 gcccaaagaa tggtgcgatc gccatggtaa tcctcaccat tgatcaggtc gtccctctct
    67561 tcagggtcgc tgctgcgtcc gagaaaatca atgtgcgcct cggcaaaatt actgatcact
    67621 actgtcggaa tggtattctg ggttggcatg gaacacacta agcttcgtcg ctctttttcc
    67681 tcgcttgatt atcacgaatt ttctgggcct tcgtcttgag gtcctggaag aattcggatt
    67741 ctacaatttc attcacttgc ttctttctct tactgtcatc aatgatgatc ctcaattctt
    67801 caacctgttt acgcaggctt tgttcacggg tatagacctt gtcaatcatc gactggaaca
    67861 ctttgtcaag ggtctcaatc tcatcgggga atttggaagt ttctcccaca ttggaaagga
    67921 aaaccaaacc tcccttgtaa tcgccttcgc cgatcttctc tgccgccttg gtaagtgtaa
    67981 gaattggacg ggtgaatgcg ctggaaataa aaaagacaag gaagaacaag gaaatatagg
    68041 tgatggcaaa ggcgatatat accttcttca agatcgctgc ctgcacttca cggacataat
    68101 cagctttgaa atccactcca atggcaccta ctacttcacc cttggagttt ttaatcggag
    68161 caaacgccga tacccagctt ccaaaatcgt ctgaatacat ttccaccttg catccaggtg
    68221 tgccatagat acagccgttg ttcttttggc cttctggcgt attttggagg gtgagttctt
    68281 ccaagccaga aagattggga ccaacattat cagtggtcca atgctccatg aatttggcac
    68341 ctgttggagg gttattcaat gcaccgccgc ttgtcacgaa gatcagttcg ttgggttcgg
    68401 ttcctttgat atacgtgtag atcccagcac ggggctcaat ctgcttgacg gaccacaaca
    68461 gtttagcttg cgcccagtag cgaaggtcat cagtatatcc atcctcacga ggggtcgctt
    68521 cagtataaag agcttgaaac tcgtcaccat caatcaactt agtggcacct tgaatcgtat
    68581 tcacaagatc atctgtgata cgctgcatag ccaggtctgt ggaaaattga taaaaccaat
    68641 aaaaggcaac agcaaacaca acagtaaaca acaaggtgaa acctatgagc agcttaatcc
    68701 gtaagctgat aaaaaacacc ctgcttttct tagtttcgtt cattttgcac tctcctctat
    68761 cgaaattgat tgaagggatt cctgcacggc ccaatgcgtt tcggataatc cataatgatc
    68821 cattagataa gctttgacac acattgaaat ttgatcaaca tctttcggat tgtaagcgtc
    68881 ccggtcaata ggataaattc ccaagtgcat cggattgaaa cctgtctttt caagctcgtg
    68941 acggctaata atctcaaggg cttcttctgg agcaggcggt tcataatact tgactggcaa
    69001 caacaacatg ccatactcct taaatcgagc cacaaccttc ggcgactcaa cctccaccag
    69061 tgaccccaaa ggcaggggct ttccgagcag ttttgcatcc attaaaagct gcttgtgccg
    69121 caattcaatc ataagcctgg caagactagt ataacctgaa taggaaaagt ttctataatc
    69181 aggatgcacc cccaagtcca ggccgaggcc gcaattcctc ttatagttgt atttaaacac
    69241 agtcatcgcg gcagggcgtc cattgaccaa cgccagccac tgatgctcta aaaatggcgg
    69301 cacaacatca ttcgggcggt ttgcccgtcg tcgcattacg gcaacgtatc gcccgtaccc
    69361 gggaaacagt tgcagataca actcgatcag ttcatcaagg taacgagtat tgccattgac
    69421 aatttcctgg atctcaaatc caagctctgg aatctcgaac atcaaaactc tatattatcc
    69481 aaaacgcttt ttctcttgga gcgtgcaaaa cgccaatcca acaggctttt ttcatacaca
    69541 cggttgaaat tgaaactcgc caacatggca cccaaagccg cgaacagggc tgtgccaagg
    69601 gccatatact gaaacgttaa ttccgtgatc aacgcagaat gcatcagcca tagtaagagt
    69661 gccatccaca tgctgctaac gatggtggca aggttataga aaacagaatc catgaaaagg
    69721 gccacacgac cgcggcgctc atctgggatc aacccctgta acgacttgcg agtaggttca
    69781 tcccaaccac gttggatcat gcgcgcaaag aaacgtccaa tcgcaacacc ccacaccccc
    69841 accaagccaa gagcaattcc gcttgcactg agaagaaaaa caggtaaaac catgaaagag
    69901 tttttcaacg caactttttc cagcaaacgt cgggtaatca gccattgaaa ggttaacaac
    69961 ccgatggttt gagcagtttt gaagtatcca agaaactgct ggaattcaag ggaattgccg
    70021 ctcgtagttg tttcaatgga acggagaaaa taaaactcaa gcagcgccaa agccataccg
    70081 ctcaagaaca tgataatcgc caccatttta agcatggcaa cttttgtaaa atattcaaca
    70141 cccacctgca tacttttcat caggctggtg tcttcatctt tggactggcg cgcacggaca
    70201 tggtgacgga aaaacgcaat tcgcaagacg acaaccgaaa caaacaacaa aagtgcagcc
    70261 aggacaaaga tcgcttcagt cccagctgaa agctgctcca acaatttagc tgacaaacct
    70321 gcgagagagt ttcctgccaa actgccaacc actgcgccag atgcaatcag aggaaagact
    70381 ctcttccctt cagtaaccga gaaaacatca ttggcaagcg cccaaaacgc aagcggcaaa
    70441 atatagtatt gctggtcagt aattatggaa agtaaaggaa aggtgatcgt atcgggtatc
    70501 tgggcgcgga acaggaacat caaaaacaga tagaacaccc ccatcactcc aagcaaccag
    70561 gtgatcaaat tcaaccttga atatcgatcc acgagaaccg aatatccgct ggcacccact
    70621 agcgtcaaaa gagtcatcac aagccacagc cacggcaaag aatcggaccc aatgttactg
    70681 acgaaacctg ccgttgcaac aacgtcagat aattccatca ccatcatgtt aatggcaagc
    70741 aaaagccaca aagccagaac cagtttaaca tgctgcgtgc caaaccagtt ggttattctc
    70801 tcattcacaa aaccctcgtc ggttaacaat tttttggatt tgaagcaagc tccaatctct
    70861 ttttttcaag attcaatgaa tggatttctg gcgccgtaga caaagcaacg gggacgcccc
    70921 cgatcttcca aattccattg aagaacgaac tctctacaac ttgaaaatgg agcaaagctc
    70981 taaaatattc tttccttttg gggaaaacta acgcgagtat acccgattat gagaaaggct
    71041 ccaattctgc aaaaatgcct gaatttagtg taataaatca ggaaaagcca tatcaaccat
    71101 taattacgac ccgaattttg gagtttcttt tttaggctca cacccttacg ctgggtaatc
    71161 attaagacaa gcaaatgtta tacaagtgaa gcctcgtgaa agtgtataat aaccttatga
    71221 ccgacgaacc catcaaatca ctcgctcgaa acaaaattgc catgctgatc gacggcgaca
    71281 acgcccaggc ggggctgctt tcccagatgc tggtggaggc aggacgctac ggccaggtga
    71341 ccgtccgccg catttatggg gattggacga cccaaagcat gaattcgtgg aaagacacgc
    71401 tcaattttca tgctttccaa cctattcaac aattccgcta caccattgga aagaatgcaa
    71461 ccgacagcgc aatgatcatt gacgcgatgg atatcctgca ttcggatgtg gtggatggct
    71521 tctgtctggt ctccagcgac agcgattaca cgcggcttgc cacccgtatc cgcgaaacag
    71581 gtgttttcgt aatgggcatc ggtgaaaaga agacacccaa accctttgtc aatgcttgtg
    71641 atgtattcgt ctacacggaa aacctgacct cagaaaagaa gacctcccca caaaagaata
    71701 gaaacgtgga taaaaccaaa aaagccaagg atgaaaagga agaactggac ccaatgccca
    71761 ttctgagtca ggcttttgaa atggccgtac aacaagatgg gtgggcctct ctggcaaaca
    71821 tgggcaatgc gctctatcaa gtagacccgg gcttcgaccc acgtacctac ggtcacaagc
    71881 aactatcaaa gatgatcagt aaattaaaag accgcttcga aatgcgcgca actgaaaaag
    71941 acggtccgat catcttttac gtacggatga aggaataaaa gcaaaatgac ctgtgtcatg
    72001 caggtcattt tgcttttaga cacaaggttt tctcgcaaaa cccaaaacag ccaaaatgga
    72061 cagcgacaca aatcaattca gccagcgcga aaaagaagtc atagaaagcc ttttacaggg
    72121 caagagcaac aagcaaattg ccctggcgct tggtatttcc ccaaacaccg ttgaatatca
    72181 cttgaaaaag atttacaaaa aacttcaagt gagttcaagg acagaggcag tattgcaatt
    72241 ggggaaatcc gtagtcagcc atactccgag tacaccacgg gaatccgcag ttgaaatgaa
    72301 cagcaaaaac gccgacaatg gcgtcaagtc tgtttcaact cggaggattt ccttgaaaaa
    72361 aatatatgca gtagtcggca ctttattgat tattatttcg gcccttgtgt tgttttttgt
    72421 taaccgacct gatccaagcg cagagggcac accaacaaac acagcaaagt tgccagatct
    72481 ggcaatcacc tctgcctacg taagcatggt tgacgaaaac ggcaattgca tcgacaccta
    72541 tgtcctgctc gttacagccg ccaaccatag tgcagttcca gtacatgatg ttgctgtgtc
    72601 aactgcaaac cattcaactc aacttgagat tctggaagca taccaaagca aggtcatatt
    72661 tttccctgca acggctacag acggcaacta cactgccgtc attgacccag aaaacttaat
    72721 tttggaaact aacgaagaca acaacagtct atccaccttt gtctccaccc caacgccagt
    72781 agcggattgt ccgccaccat taatacaaac cctttcaaat aacggcagcg cgacccaagc
    72841 cccctacccg cttcccacag ctacacccgc tgcgcctttc attttacttg ccagtcccac
    72901 attgctgttt acagactcat ccacaccaat atcagagcgg attgttcact actatttcat
    72961 aacacctgct gaaaatcccc ctcccgacgg aagtattgta gtcatatctc atatccttgc
    73021 gccaacaccc tctgacagaa catacggacc cgacacggcc acaaacatca gagcatcgct
    73081 tgaaaatgtt ctgcacgatg aaagaaatgg ctggacaagc accaacgtgg caatagtgga
    73141 tgtcactttc aacaacggcc atacaaacgt gattttacag ggcgagtatt ttggtgttgg
    73201 cgatgtcacc ttgatcgccg cacgtatgca aattctcatg acggtatttg caaacccctc
    73261 tgtacagact gccaccgtca cactcaacgg agacacgatc ggcaacatgg gcatatccaa
    73321 cagccttgaa gccaagcctg ctggatatat atttacccgt tctgaaattg agactttcca
    73381 gcgcgaccat gtgtatggaa caccatgacc gcgctggaaa taagaattaa ttggttggac
    73441 aaaccgaatc acccatagca ataatattag ccccactgac tgaatacacc ataaacagac
    73501 acccttcata aatttcagga tttggaccaa attgtgtcac ctgccacctt ccactggcaa
    73561 tgacaaacca cacccttgtt ccctgaggcc aggtaacaaa atcgccctcc cccaagccga
    73621 tcttttggtt ggcctgttca taactcatct cttcaacata caagacacgg gggccttcga
    73681 gccagttcgc accatccaat gtctccagaa gctgtatcgc tgtggcttca gccgcaaacc
    73741 ttgcatcaac gaaggcaaca gaagaaggcg gcaaatcatt gcttgttggt tctgaaacgg
    73801 ggacgttgtt tacggtagtc tgaggcgttg aacaacctgc cacaaagaca aatacacttg
    73861 aaagcaaaaa tatataaaga ggtcgcatgg aaagcagacg caatgaatac ggcgtttgtt
    73921 ccatgcgacg gcacgaaaac tggcctaaaa gttcttaaga acgtacggca tcagagccag
    73981 tgagcaaaac accaacactc cgcttctcca ccagcccttt gtacacatcc acgatctggg
    74041 cggcaatatt tgaccaggcg tagtccaatg catattccgc cgcacgcagg cccatcgttt
    74101 cgcgcagatg cgcatcacct aataatccag ataatttatt gcacaatgcc tcagggtcgc
    74161 tatcgggaat ggtgtatcct gtctccccat cctgcacaag ataaccgagc ccgcccactt
    74221 cagaagcgat gacgggcgta ccgcaggcca tggcctccaa tgccaccatt ccgaaagact
    74281 cgtataacga aggcatcacc agcacctccg ccgccgagta atagtatggc agcgtatcct
    74341 gcccgcgttt gccaagaaaa accaccatgc cgcccataca caggtcgtca cacaattttt
    74401 gcaggcgcgc catttcagcc gacatttttt cagggatcac atcaggttca ccgccaataa
    74461 tcgccagatg cacaggttta tgaatttcat tcacccccag acaagacatc gcctgaatca
    74521 atgtgtccac acctttcaac ggctcaatac gccccacaaa aagcaccatg cggctatcgg
    74581 gcgtcaggcc aatgtactgc ttcgcctcat cggcaggaat gggataaaaa tgactcgtat
    74641 ccacccctgg cggaatgaca accagtttgc gggcattcgc acggtacagg aatctcaact
    74701 gggtcaactc agccaaagtg gcgacaacga tccgatccgc ccgaccgaga acctgcctct
    74761 cgcctttgag tcggtcttcc cctgcacgtt catcctccga acgcgccacc cgattcttca
    74821 tctcccccaa cgtatgaaac atgtggacaa tgggcgtacc gccccaggca tcgctcaagg
    74881 cctccgccgc caacccagac atccaataat ggctgtgaat gacatcgtag tgaatattct
    74941 tctcagcggc aaattgtttg atgccctcaa caaactcagg gatgaatttt gcaatatcgc
    75001 tcttgctttt cggctcttca ggccccgcag ggatatgcac cacacgattg ccaaatccca
    75061 attcatgcac aacatgcggc acatgctcgt cctgagagcg ggtgaacaca tctacatgga
    75121 tccccatccg ccccaattca caggtcagat cacggacata tacattcatt ccacctgtat
    75181 ccttgccgcc aagtgtggcc aaaggacagg tgtgatacga taacatggca atacgaagca
    75241 tattttctca attctccacg gtacagacga cgcgaaacct cgaaaacttg cgagattaag
    75301 cctaatcctt tataatcccc gccgcttgaa aataagccac catagctcag ctggtagagc
    75361 aaacgattcg taatcgttag gtcgtgggtt cgtatcccac tggtggctca agtgcatcac
    75421 cccctgcgac ccgcaaggct cagaccttac gggttatttc ttttaaagta gaattaccat
    75481 atctggctcc atgaataaat acatctccga aatactcaaa ataaagacgc gatcgttcaa
    75541 tgcgcgcgag ctcgtgctgg ccaccctcgt cactggggtc gtcggtgcgg cgctcatggc
    75601 tgccattaac atcaaccgtg ctgagatgat cccgctcagc ctgttgctgt tcatcgctct
    75661 ggttttgggg tatttcggat acttaaatgc cgctcggtgg gtttcgctat tcaccacact
    75721 cgccatcatt tcgagcctgg tttataagaa taatggcatc cgagatcctg ccattctggg
    75781 tttggtggtt gttttgatcg cagccgggct gctggcaggc cgcagaggga ccattattgt
    75841 gggggccctt atccttgccg aggtgatttt gttcggcttc ctcgaaacac atggatacat
    75901 cacaaacaaa ttcagccaat tcaacacctt tgccgattac ttctccacaa gcctgtgtgt
    75961 ggtgctggtg accgtctttc agtggcttgt catcgcccgc ctgaatgaaa atatcaaaac
    76021 cgccgagttg gaaatacgcg aacgcgagac cattgaaaag cagcttcgtg atgccgagac
    76081 gcgctaccgc aacctggttg agcaaatccc agcggtggtt tacatttccg agcctggggc
    76141 ggcgggcatt tggcattatg tcagcccgca gatcgaggcg ttgacaggtt tcactcccga
    76201 agaatggacc ctcaacaaag acctctggta tcgcctcatt catcccgatg accgtgaacg
    76261 gaccgtggaa gcggagctct cctcgctcaa cagcggacaa atgccccgca ccgaataccg
    76321 cctgcaaacc cgcagcggca attatgtctg ggtgcaagac caaagcatcc gctccatgga
    76381 aaataatgtc ctgcagggat ttctggtgga tatcaccgcc cgcaaaatgg ccgaagatca
    76441 attgcaaaaa cggctggccg aattgaatgc cgtccgcggt gtgagtgaga ctctcattgc
    76501 ccaaaccgac ctgaaagaac tcatccaaca aacaggcgac cagatcaagg tcacattcaa
    76561 tgtggataac ctctttattt ccctgttgaa tgccaccaca agcctgatcc atttcccgta
    76621 ttatgtcgag aaaggccagc aaattcaggc tccctctctg actttcggca aagggatgac
    76681 ctccagtgtc ctgcggacga aaaaggcatt cttgatcaat acaaactggg cacaagtctc
    76741 ggcggagtat ggcgtcattt actacgataa cagccccgcc cgttcttccc tgaccgttcc
    76801 gctgatgatc ggcgaaaaag gcattggcgc catttccgtc cacgatatgg aaaaagaaaa
    76861 tgccttctcc gaaaatgacc tgcgcctgct ttccaccatc gccgccaacc ttgccgttgc
    76921 aatcgaaaat acccgcctgc aagaatcgct caaacgggaa ttgaccattc aggaaaagct
    76981 ggtgacggaa ctggagcaaa agaacgccga gttggaacgt ttcacctaca cagcttcgca
    77041 tgacctcaaa tctccgttga tcaccatccg tggttatctg ggctatcttg aaaaagatgc
    77101 ccgcaacggg aattttgaca ggctgcaaca ggatattggc cgcatttccg aggccgctga
    77161 aaaaatgcac aacctgctta atgacctgct cgaactttcg cgggttgggc acatcatcaa
    77221 cgaaccgcgc aacatctcat tttcagaagt cgtggaagaa ggcttgaagc gcgtggaaag
    77281 ccaattgaaa gagaagcaga ttcaggtcaa gatcggaagc gacctgcctc agatcaacgg
    77341 cgacaaggaa cgtctcatcg aagtcattca aaatcttgta gagaattcct gcaaattcat
    77401 gagcgaccag ccgaaaccca tcatcgagat cggtgtcgaa aaacgggatg gcgaaaatat
    77461 cttcttcgta aaagacaatg gccccggtat tcgccaggat ttccatgaac gtgtgtttgg
    77521 gctgttcgac aaactcgacc ccaccagcag cggaacaggc atcgggctgg cactggtgaa
    77581 acggatcgtt gaagtacatg gcgggcgcat ttggatcgac tcgtacggct acggctcagg
    77641 aacgaccttt tacttcacac tgacagacaa atcatcttca taaaataccc tgatttttca
    77701 gggtattttt ttcctattga caaatatatt acatatacta taatactagt attatagtat
    77761 atgtaatata aaactacacg caaggaacaa aaatgtctct aaagcatgcc attctcggtt
    77821 ttttatcttt tgccccgttc acaggctatg acctgaaaaa agccttcgac aactctgtac
    77881 agcatttctg gccagccaac caaagccaaa tctaccgcac tctggccgaa ttggaagaaa
    77941 atggatttgt agaaaaagag gtcatcgccc gcgaagaacg gctggatatg aaggtttatc
    78001 acatcaccgc cacagggcgc gaagaacttc atcaatggct tgccgccccc ctgcccgaac
    78061 gcgatgaccg tgaacctttc ctcattcaag tctattttgg cggtcaactc aacgacacag
    78121 aattgctcaa agtctttcaa gacaaactca aaaaaatcga agagcaactg gctctgtacg
    78181 aagcggtttt tcaaatgacc cagaacacac cttcaaaggt cagcgaccca cggaccgttt
    78241 tcctgagcat gcttaccctc gaagcaggat acatcaacac gcaatccatc gcggcatggc
    78301 tgcgctcagc cattgcacgc attgaaaata aaaattacac gatcaacatg ggaaaataaa
    78361 tgaacaagac cattcgcaac attatcatcg tttcactctt tacaatcggc ggcggctggc
    78421 tgggcatctg gctcaacaac gtcacaggca acacccaacc gcccatgcaa agcctcggcg
    78481 tactggtttg gcttaccaca cctgcctttt caggcttttt actacgcgct ttcggaggcg
    78541 atggctggaa ggattctggc tttgggctaa atctcatctc aggctggaag tggtaccttc
    78601 tcggtatcct cgtctacccg ctcgcttcac tcctgacctt tggtctgggt gccctatttg
    78661 gcatcgtcag cgccgaaggg cttgccgccc agggcttcaa cgcctatctt tctgcagcgg
    78721 gaatgatatt tgtgggttcg ctgatgaaaa actttttcga agaatttgcc tggcgtggtt
    78781 atctcactcc ccgcttcgaa gccgcaaaac tccaccccat gctgaatcat ttcatcgtca
    78841 gcctgctctg gatgacctgg catctgcctt attactatta ttaccttgac cgtgccaccc
    78901 tcaacagcgc cattacaacc agcattcctg tttttctgct cacgggctat cttgtcatgt
    78961 tccccacagc gattctcttc ggtgaaatca ggttgttgag caaatcggtt tggcctgtgt
    79021 tcatcctgca taacataatc aacgccttga gcatgcccct gcttatcaac ggatttatcg
    79081 aggtcaaagg tgcattggct cccgtcttca cccccaccaa cgaagggatt cttgtttcgt
    79141 tggtgttcgg tgtcgtcggc tggttcttga tgcaatatcg gcttaaaaag caggtcacgc
    79201 cttaagcgtg acctgtaaac aacttttact caatgccatc gaaaatgtga tcaagcacat
    79261 cttcgctcac atcaaagacc gatccgtttt cagggatggc ttcttctgtc atccacttgg
    79321 gcaggcgatc atcttctttc gtaaaaccag cccgcttgtt gaactcacgc tccaacttga
    79381 tggtttccct gcccaaggcc tgcaaaatat tgtcgggcac atcttcccag ccataacgtg
    79441 atttgagcag tcgttttacc actccatcgg gcgtggcggc ataaccaaag cccgcgaaaa
    79501 tacacgcacc gatggtgtcg taccctgcca tattcaattg ggcattcagc gaagcatctt
    79561 tttgctgggt cgggtccagg tgattgacct gcgcacggat ggtgaggccg caggtatggt
    79621 cagcaccttg aggagtggtt gcgtacgtaa caccagtccc cttgatggaa cgcggctcat
    79681 acgccgacat ggcttggccc ttcacagcgg ggacacgctc cacaccatat tttttgccaa
    79741 ccgccacggc gccatcgcca aggacccgtc caagttccgt gccctgacgg atctcatcga
    79801 tcaacttcag gccatcctct tcgctgcccc aacgcatcag gcctgcttca gcggcaacgc
    79861 ccaaagcccc gccaacctca atggtatcca gaccaagatc attgacctgc cagttcatgc
    79921 gcccgatcga gtcgagcgaa tcaatatcca ggttggtgcc catcaaacca accgtctcat
    79981 actccaacgg tgaaacgatg atcttgccat cctctccgcc gaaaacgttt gagcatttaa
    80041 tggtacaacc tgccatacaa gcatgagacg ggtcagatgg cttgccgcga ttcaaaatga
    80101 attcacgcat ggtctcgccg cttatttttt ccacatcgta gaaagtgccg cgtgagaaat
    80161 tatgggtggg caacgccgca aaacgctgag tcatctgcgg catggcctgg gtgccataat
    80221 cacgatacgt aatggattgc gggtgattca tcacagactg ggtgtaatcc ttttgcgcca
    80281 ccttgaatgt ttctgcatct gcaattggag gtttctgacc gcctgcatga tcgaaaacga
    80341 tcgccttcaa tccctttgaa cccatcactg cgccaaggcc gccgcgagcc gcgatacgcg
    80401 aagggatttt gtccttgtcc aggttttgga tccctgccga cttcatacgc atttcaccac
    80461 caggcccgat catggaaatg gccactttgt cgccatattt ttccaaaagc ttcggtgcgg
    80521 ttgcgtaaac gcccagccca gccaggtcat ccgcgcgttc ccattttgcg ccattcagcg
    80581 agaggtgcaa tacccaatac ccgtcttcct ttggcatatc ttcaataatg agcgcatgga
    80641 tgcccatgaa ggccatgtga tagcctgtgc gcccacctgc attggcttcc ttgataccgc
    80701 ccgtcaacgg agattttccg cccaccgaaa tacggtctgt ggaagaaagc atgtgcccca
    80761 ccaataaacc tggcgtgaag atcagtttat tgtcagcacc aagcggatca cattttgcat
    80821 ccacctcatc gacaaggatg cgcgcgataa gaccacgccc gcccaaacgc tgccatgttt
    80881 ctgggatcgg ttcacgtttg agttcctggg ttcgggtgtt cacccgccaa atttgagatt
    80941 tatattcagc cattttgact cctgtgagca ggattatatt cgcatttatt ttcaaaaagt
    81001 ttgcaaaacc cgttatacca gtaacgaggt agggttaagc aaataacgag gccactgctt
    81061 ctcgaaacac cctggtgtga tgatccacat cggcctgggt ggtttctggt gaaatcaaag
    81121 ccatgttgtg gaagggggtc atgagaattc cgcggttcag ggcatagaga tgcatgtaac
    81181 gatcaagttc aggatcaacg gcagcatggg cttctccacc atttttcggc gggttggggc
    81241 ggaaccagta ttcagaacgg ttgcccaact gcttcacgat ccacggcaac tcgaattcct
    81301 tgatcacgga ctgcacccct gcggtaaatt gctcctgcaa ggcggttgct tttgcgtaaa
    81361 actcatcggt caacacgtgc tgtaaagtgg ctttcatcgc cgcaatggac aaggcgttac
    81421 cagagagtgt gccaccaatt ccgcccacat cggcatcgtc catggcgagc ttggcggcaa
    81481 acgccttgga gacttcttcg gtgaagccat agatcgcggc agggacacct ccagcaagag
    81541 gctttccaag ggttaagaaa tcaggctcga gggagtgcga agcggtgtat cctcccgggc
    81601 cagcacagat ggtgtgcgtc tcgtcaatga tgagataggt gccgtatttg cgcgtcaatt
    81661 cacgcagggc tttgtgataa ccagggtcgg ggtgaatgat gccaatgttg gtcatggcag
    81721 gttctgccag aacaacagcc acatctcggg cggagagggc ggtttccaaa gcggcgatat
    81781 cgttgaactc aatgaccttg ctagtctcgc gcggatcaat ctgcggcccc atgttgttgg
    81841 gacgtgagat gggcgtacct tcctcatcca gcgtgataaa ggtttcgtcc acggagccat
    81901 gatagcaata gttgaaaacc aacaccttgg atcggccagt gataagacga gccatgcgaa
    81961 gcgcaaatcg gttcgcatcg gtggcggtga gggcgaattg ccaatagggc agtttgaagc
    82021 ggcgttgtaa ttcctcaccc acccagatca catcttcata cggaagcatg agggtaatcc
    82081 ctttttggat ctgttcagtg atggctttga ccgtggcttc gggcgcatga cctgtcatgg
    82141 cacctgtatc gccaaggcaa aagtcaatgt agtcgttgcc atccacatcg gtaaagtggg
    82201 cgcctttggc ttccctgaca aagacaggaa aggaacccgc ccaacggatc atccacaaca
    82261 tcggcacgcc accatgcagc gacttgcgcg cacgctggta catttcgtaa gatctcgggt
    82321 gattcttgtg aaacagttcc tcttcctgct tgagaagtgt ttcgagtttg gagcgatcaa
    82381 gtgattttgt cattttgtgt ccattgcaat tgaattttta ggattgccaa tatatcgaat
    82441 caactttata tttcagtatg ggcgggtatt aagcaaaaac ccgcaccttc taccgaggaa
    82501 aacatggaaa acactgctac aggaaccttg ctcgatactt tcaatcaaga caaacgtttc
    82561 agggtgccag cctactacac ggtctgtttt ggcagcctgt catccggcct gcgcttctcc
    82621 gtctttactg acaggaccag agcgcttgtg gacgatatca ttgactgcct ggaccttgaa
    82681 agatactcgt tcattacgat cttcaaaaac tgggctacag aagatttcaa gaacgcgcgt
    82741 cccattgacg attatccata cgagtatctt ttcaaaagcg acagcggcca aatgctggtc
    82801 catctcattg cccgtgaagg gttcatcacc gtggattttt attacgatgt gaaccgcccg
    82861 gactgcgaga agtgggtcgt tgaaaccaac caaaacctgc gggaacgctt cagccctgcc
    82921 aagcagccaa aattcaaggt gttgatgcag caagagggca tgtttggcac tgaagatgtg
    82981 aacaccaacg acttcgagtc catcaacctt gcagaatatt acaacgatga tttcctggag
    83041 atcgacgcaa caatctcaga ctttatggcg aagaaggaat cgggcatcat tctgctgcat
    83101 ggtgaacctg gcactggaaa gacaacctac atcaaacatt tgatttcaag gttccatgac
    83161 aagaatttca ttttcgttca aaacgatttc gtccgagacc tgctgaaacc gtcttttgtt
    83221 tccttcctgc tccagaacaa gaatgcatcc ctgatcattg aagacgcaga aaaggtggtc
    83281 gtatcgcgcg ataacgcatc agaggattcg gtcgtctcga cgattcttca gttgacggat
    83341 ggtttgttca gcgatttcct caacatcaag atcatctgca ccttcaacac ggatattgaa
    83401 cgcatcgaca aggctctgct gcgaaaaggc cgaatggtcg caaaatacaa ctttagagcc
    83461 ctgtctgccg agaaaacagc cgccctcgcc aaaaagcttg gacatgatga cgtaaaagga
    83521 agcctaaccc tggcagatat cttcgaattc gacaagcgcg acttcaaaga caaatcaaag
    83581 aaagtgatcg ggttcgtcta acttttagaa ccaagtcacc agaagaagga gatgggaatg
    83641 tacggcccat ctcctttttt ttacaccgat tccttcaagg cctcgccgaa gatcttcaag
    83701 ccatcattga tctgctggct ggagacattc aaaggcggaa tccaacgaag ggtattgtcg
    83761 taggtgccgc aggagagcag gagcatccct ttttcttcag ccttgtgaat gatctctttg
    83821 acgagcggtt tcgccttggc ctgcgtaccg tcaacaatga attccgtacc gatcatgagc
    83881 cccttgccgc gcacatcgcc aatttgcgga tactcttcct gcaactttcg caggcctgtc
    83941 attaactgaa ttccgcgttc ggaggcgttc tcgagcattt tttcttcccg catggcacgg
    84001 atggtggcaa caccagccgc acaagcgacc gcatttccgc cgtacgtgcc gccgatggat
    84061 cctgtttcaa ctttcttcat aatgtcggtg cggctgaaaa cacccgaaag cggcattcca
    84121 gaggcaattc ccttggcggc ggtgatgata tcgggggtta catcaaaatg ttccagcgca
    84181 aaccatttac ctgtgcgccc aaaaccagac tgcacctcat cgaagatcag cataattcca
    84241 tgcttgtcac agatctcgcg cagccccttc ataaaggatg tcggcgggac aacataacca
    84301 ccttctccca aaacagattc gatgagaatc gcggccgtct ctttcggcgc tgtttgggaa
    84361 gccagcaaat actccaagcg atccagcgca tattgagaag cctgctcctc cgtcattttc
    84421 aaggcgaaag catagggaaa tggggtgaca tatacacccg aaggcagggg agcaaagcct
    84481 gtgcggtaga tggtcttgga tgtggtcaaa gccatggtgg catgtgtgcg cccatggaag
    84541 gagccattga aaacaatgac gttgggtcgg cccgttgcca cacgcgcgat cttgaccgcg
    84601 ttctccaatg cctctgcacc tgaattggca aaaaagaaac tatcgagcga aggcgggaca
    84661 attttgcgca gttcgtcaat caactgcagc atgggtttat gaacaacaat attggcctgt
    84721 gcatgcaaaa acaacccagc ctgctcacgg atggcttcca cgaccttcgg atgacagtgg
    84781 cctgtgttcg tgacaccaat gccgctggtg aaatcgagca gctttttgcc gtcatctgta
    84841 taaatatagg cgccctcagc acgttctgat acaaaactga aaatacggct ccaggcgggg
    84901 gtcatatgag agaaattatc ttgatagagt tggttcatgg acgttccttc taaagcaaaa
    84961 ttttgcaatt accgaaaatt ggagatgaga ctcatcatcc catctccaat ctctaattac
    85021 aaattaccag ttactactat tcgctttcta tgcgggcagg tattccgcgg cccactcaac
    85081 atcatgcttc ttcaaggtct cacgggtggg aacgccgtcg ttggtccagc ctgcgagctt
    85141 gtaataagtg tcgatggcag aatcaacttc ttcgtgagtc agagcgaagc cagccgtggg
    85201 gccagtcccc tgcaactgct tgaagaattt cttcggcagt ttatccgcat tgcgggtgag
    85261 accttcgcgc gcgttaaaca cgcggaaaag atccaaacgg cggcggccaa cttccatcag
    85321 ttcttcaacg gtgatatccc aaccagtgac tgagccgatc atgttggcag tatccttgcc
    85381 gtcatagagg gtccaggtgg ggccgtacac aaactgacac agttcagcgg aatcgagcat
    85441 ggagtagaaa acctccgtct catacgcaaa gcggaccttt tcctcggtga ggctgtatgc
    85501 gggttggggc gaaccgagac cgatctgctt gaggcggtcg aggttgaaat cgccaacacc
    85561 ttcttcgtag tagggatcgt gctcgctgga ttggtgatcc gcgccgaaag gattgaccgc
    85621 gtaaatgagc gcaagcgaac gcttggcctg cggcatgtgc gcaggagctt ccgcaccttt
    85681 gaccgtgatc aggttatctt ctgcgccatt gccccatttc tttgccgcac gttcagaacc
    85741 catgccaaga gtcttgccaa actcaccttc gcctttgacc agcatgtaca aagccttgat
    85801 cattgcatcg gcattgccaa acttgaggtc gaggccagca gtttgttctt tggtgataac
    85861 acccttctcg taacattcca ttgcaaaagc gatggttgcg ccagccgcaa tggagtccac
    85921 ggcgtattcg ttacagatct gattcgcaag cgaaaccgcc gcgagatcat caataccaca
    85981 gtaggaacca aaagtgccaa gggtttcgta ttcggggccg ccatatcgcg gatccacttt
    86041 gtacgcgccg tccttgatct ccaccacgcg cttgcatttg accacacaag catagcaggt
    86101 atcacgttcc ttcagaacgg tctcagccat cttttcgccg ctgatcggct cacaaccttc
    86161 aaattgacct tcgttgtagt tacgggtggg caatgtgccg atggtattgt tgaacaacac
    86221 cacaaccgcc gtaccgaatt tcgcaaggcc gtccatatct ccattttcag gaatggcctt
    86281 ggggccgatg cgattcaact cggtcaatgc tttctggttg gcaagctgaa ccttcttcgt
    86341 gccacgcacg accacggctt tcaaattctt tgaagccatc acaagcccca tccccgtgcg
    86401 cccattgtgt cgattggaca ttgaacacag cgaggagaac aaaacaccgt tttcagcacc
    86461 aatgccatgc tgcaaaattt cagccttcgg gtccacttcc ttgtggatga tgtcatccac
    86521 ttctcccgtc agtttaccca tcaaatgcgc cgcatcacgc agttcatagt ttccatcaat
    86581 aatggcaagg taaacaggtt tggaagattt tcccttgacc acgatcccat caaaaccagc
    86641 aaatttcaat tcagcaggaa agaaaccacc gctttgcgaa tcgccaatac cgccgctgat
    86701 cggcgacttg gcatttgcat tcaaacggct ctgaccagaa atagccgcac ccgtggcaac
    86761 cccggtgaac agggtcaaca cattatcggg gccaagcggg tcggcacctt tgggcatatc
    86821 gcgcagaatg tagtgcattc ccatcgcgct gccgccaagg tatttgcgat agaaagcctc
    86881 gttcggctct tccaccgaaa gcgaaccttt cgtaagatca acgtgcaaaa tctttccgtt
    86941 ataaccgttg ggcatgagct cctccgtatg aatatgggtg ttccgtgtag aacgtcatca
    87001 aagcgcatgt tcaatgcgcc gatcattttg tttgttggat tataacaagc gctcaaaggt
    87061 tcgcacaacg ggaaatttcc gaaagcgaca gagattcaac acaaataagt tttttcttac
    87121 acgacccgcg ccgaatcacg cttaactgga agttgattcg gtttcgaggg gcatttccct
    87181 gcgtttgagc acgtcttacc tgcttcaatc ttcctcgccc gccacaaacg gattcacatc
    87241 tggaacgccc ggcttgatcc attttcgaat tgggaagtac aaaatcgcgc caactgccat
    87301 tgctcccaac gcccatgaaa tggacgccat tccctcttcc acatactggc tgtaaatggc
    87361 aagtccaatg atcatcgccg gagcaattgt caccagggtc aggccaaaat acccgcctgg
    87421 tacttccttt cgttccaaat gcggcatggt gaaacgcagc ttccacatgg cgaaaaattc
    87481 ggccatcaaa accagcatat tgaggaacac atcgatcaca accaaagccg caaacgctga
    87541 aagtgaaaag atcgaataga tcaacccgca aaacaaaatc gccacccagg gtgtgccata
    87601 tctcggatgc accttgacca tgaacttcgg catcatccca tccgctgcca tcgcaaacgg
    87661 aacgcgggtt ccacccagcc cattgccaat gaacaaaccg atcatgctca gcactgccga
    87721 aaaggtgacc aacccaccca tgaccagagc aagcgtcccg ttggtttgac ccgtcaattt
    87781 atccgccacg acatgggcga tatcgggaaa ttcccatccg accgagctgg ctgtatccac
    87841 gccccaggat tgggcctgct cagcagggac accgtactca tccaatactg gaccaatacc
    87901 ctcgccgctc tcatactcct cgatacccca catttgatag cggccatctt caccagcacc
    87961 gccatacaaa cccgccaaag taggcaggga atatgtagca atggcagcca ccaacaccaa
    88021 aaccatcgcc agcggatagg tcttcttcgg attgacgatc tcatcgccag cagctgcagg
    88081 caattcccac cccatgaaat tccacatggc tacaaaaatg cccgtactca acgcgccgat
    88141 ggtattgctg aaattcacct ctgcaccacc aggcaaaaag ggaaggtgaa tggtctcacc
    88201 gcttttcagc cacgccatga acccgatcac agtcagggca atcaacggga tcaacatcac
    88261 caccccaagc cagttggtca gcagacccga gaaacgtgca ccgcgaatat tcaaagcaga
    88321 gaccgcccag atcaacacaa cagccacccc aaacgaaagc caggtgctat caacaaaaac
    88381 accgccgatt accgcaccct cacgcaaagc agggatgaaa taaccaagat agtacgccgc
    88441 cagtacggga taaatggata catccaccca tgaaaccacc caattcatcc acccacccat
    88501 atagccgaca aaaggaccaa aggcaacctt cagccagtgg taatagcctc cctccacagg
    88561 catcatggat tgcatctcgc gtaccatcaa catattgggg atgctgaaga aaagcggggt
    88621 caaaatgaac agcaggagcg ccaaccccgg tcctgaccaa ccgaccatgg gttctgctcc
    88681 aaatgcccca ccgcacactg tgaaatagat caacccgaaa agcggcaaca gcgtcagttc
    88741 gcgcttgagt ttcttcgtct cagcgctgat cacatttccc tccccagaat tgtttttcat
    88801 tttgccctcc gaaatttttt attgcactga aaaaaaatta gcggacaatc gctctcacga
    88861 ttgtccgctt tgtttacttt atgcggccat ttccttgatg ccgtacggga agccgtaatc
    88921 ggccattttt tccatgaatg gatcaggcgg gaaggcttcg gggccaagaa caccaacccc
    88981 atcccaaatg ccatgctcca acaaatccat ggcaatgact gcgctgaagg cggtctgtgc
    89041 cacaacagcc tgacaacccc aagccttcat acaggctttg ttatcagcca cctggtagag
    89101 atagacctgg cggggtttgc catccttggt gcctttgacc cacgtcccag cgcaggtttt
    89161 acccgacatc ttatcgccga gatgagcagg gtcaggcaga caggccgcca ctacatcacg
    89221 cggcgcaacc tgtacaccct tcacgttgat cttctctttg ttatcaagac caagcatatg
    89281 caaggtcttg agcacaccga tgaactggtc gccgaggccg tatttgaagg tggcacgttt
    89341 ggtcttgacc cagcgcggca tgagcaacac ttcttcatgc tccacgttga ccacttccac
    89401 agggccgatt tcggggaaat caaagacttc aggctcagag aaaggagcgg tggtaaacca
    89461 acccttgcct tcctcccaga tcacgggcgg attcaaacat tcttcgatgg ttgtccagat
    89521 cgagaagttg ggcgcaaact cgtaaccttc gatgactata tttgcaccat cacggatgcc
    89581 aagttcttcg atctcatcga aaaggtgttc agcggcatat cgggcgaaca catcggccat
    89641 gccgggttcc acaccgatcc caacgagtgc aagctgtttc tttttcttcc agtctttggc
    89701 tttggcaaat tgatagtcac ccagcttgac accgcacttg ttaaacgggt ctttggggtg
    89761 cggtttggag agggtcattg ccatatccat gtacatcacc ccggcggaat aggcggcatc
    89821 aaagatctcc tcgttgaaga tcgggtcaac cgcattcatg atgagatcga ccttgtattt
    89881 ctttgccagt ttgacaattt cggacttctt gctggcatca atgaactcta cgggaaaacg
    89941 cttcttttca ccgagtttct tctgaacttc ctttgcacgt ttgacgttgt aatcagccag
    90001 caccatttgt tccatccagg cgcgcggctt tgcaattgcg gcaattgcct cacccactcc
    90061 accaacacca actagcaata ctttcatgag atctgattct ccattttgtt ttataacccg
    90121 catctgcgag ttagattgac cgaaaaagaa tcccgccgtt tttatgacgg cgggatagac
    90181 tgtgactacc gatattgtcg aggcgattca tttccaggga aaatggaaat ggagagggag
    90241 gtcatataca ctttcaagaa ggtatccccc acagtcttta atacacgaac tagggctttc
    90301 attagaacct ttcagttggc tctgcggtct ggactcattt ccagcgcaga caaagttttg
    90361 gaacgccgca gcaggcacga ggtccgtact gaggcgggat tcccattaga aataaatttc
    90421 tttcatgatc ttcaaatgga tgaaggattc cgtttcacgg atcccatcca cttgggcaag
    90481 cttttcggtg aggaatttga gcaggtcttc atgatcgcgg caaaaaactt cgcagacaat
    90541 gtcgtatctg ccgctgacaa cgacgaggta gacaacctca tcgattttcg aaatttcctc
    90601 ggcaacttgc agcattttgt ggccgtcggt atggatgccg atgagggcca tggtgcggat
    90661 ccccatcatg aggggattgg tcactgcaac gactttgaga taacccaact cgacaaggcg
    90721 gttatatctt tgccgaatca tgccgggtga gacgttgagt ttctcggcaa tttgagcgaa
    90781 agcttcgcgg ccatcttttc gcattgcatc gataatgtat tggtcaatgt tatcaaggcg
    90841 gctggaagga ttaccactac cgattaaact ttccattgat ttattctatt ccttttttgc
    90901 ataaaagtca atggttttat aactatttgt cataaaaaca ttattttttg taacgtaaaa
    90961 gacgtcgttt tcagcgacta tgcgcaaaat ccgtggggtt ttcagcctga tccttgaatt
    91021 tttcagagca cggggaaagg gattatacaa cagggagtta taaaaaataa cctcaatggg
    91081 caaactaccc attgaggtta ttgtactgca tggcgacggg aaaatcccgc caaattattc
    91141 tattgtccgc ctttgacttc cacccaaata tcatcaaaga gcggggtggc atcgccaaca
    91201 tcttcaatgc cgtggccatt tgcaaccgct tcagcaggcg ggttcgtgat cggagaatcg
    91261 gcgtaggcct tgtaaacatc aggctggttg gccttggcgt attccagtgc agccttgttg
    91321 gggttggtat actggaaatc gcggatggtg agccagaaga catctccctg catggtgtag
    91381 ttcaaccagg cgtaaatggc atcggtgtga gaagcaccta cgggaatagc ccagttatcc
    91441 tgccaaagaa tggggccttc agacggatag acgtactgaa tatcggggtt ttcctgattg
    91501 gcaatgaagg cttcgccggt ccaggtcatg ccgaggtcaa catcaccagc gataagggcg
    91561 gtcttggggc tgtcgctatc gaacactttg atgttgggaa cgagctcggc cagtttggct
    91621 tttgcttctt caagctgggc agggtcggtc gtgttgacat cgtagccaag ggtcaacagg
    91681 gtgaggccga tcacagcgcg ggagtcatcc aggaagatca tgcggcccgc atattcggga
    91741 ttccagaggt ctgcccatga agtgggaacg gtcttgacgg ttgcagtgtt caccacaatc
    91801 gcatctgtgc cgcccaagta tggaagggtg tacttgttcc ccgggtcgaa agattgatcc
    91861 atccagcccg aatccatgtt ggccatgttg ggcagtttgg agtgatcaag ttcctgcaaa
    91921 agctgctgac ggatcatcag accaatgatg tagtcagtag gttgaacaag gtcgtacgaa
    91981 gagccgccag ccgagatctt ggcatacatt tcttcgttgc ttgaatattc atcgcg
//
LOCUS       DAOQJC010000002        82326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_105_length_82326_cov_9.325364, whole genome shotgun sequence.
ACCESSION   DAOQJC010000002 DAOQJC010000000
VERSION     DAOQJC010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 82326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 82326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82326
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105_length_82326_cov_9.325364"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1188
                     /gene="hppD"
                     /locus_tag="PLE39_00425"
     CDS             58..1188
                     /gene="hppD"
                     /locus_tag="PLE39_00425"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HNE66815.1"
                     /translation="MATKTIDETRPMTEEADFLQIKSIDHVHFYVGNAKHAMYYWWKA
                     FGFKPVAYSGLETGNREFASYVLESGQARIVVSAPYGPSSPLASHHMVHGDGVKVIAM
                     EVDDVEKAWQATTSRGAKSAWAPREEKDDHGIYRTSSIYTYGETLHTFVDRADYKGVF
                     APTYRPIKDKEAHSTGLAAIDHIVGNVQLGKMNQWVNFYHHVMGFRQLMHFDDKDIST
                     EYSALMSKVMQNGNGRVKFPINEPAEGKRKSQIEEYLDYYLTPGAQHLAIITGDILTT
                     VDQLRKNGVEFLRVPDTYYEALPERVGSIKEDYKTIQELGILVDKDDEGYLLQIFTRP
                     IQDRPTMFIEIIQRHGAQGFGKGNFKALFESLELEQQRRGNL"
     gene            1260..2153
                     /locus_tag="PLE39_00430"
     CDS             1260..2153
                     /locus_tag="PLE39_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNE66816.1"
                     /translation="MIFATCRTPHTAPRPALVSNDRIYPLPLADMHAVIALGADKAAS
                     LASQESFAPAEVQFLAPIQPASLRDAYAFEQHVRTASQNRGRDVPQEWYKFPAFYYTN
                     PRTIFGPDEVIPNPHYTSAMDFELEIAVIIGKSGRDIKPEDAPAHIFGYTIFNDWSAR
                     DVQREELRIGLGPAKGKDFASSLGPVIVTSEALADRAVNRPGVYDLMMTAKINGGEMS
                     RANLKDIFWSFGEIIARASDTCELQAGDVIGSGTVGTGCLLELTKGQGPWLNAGDVVE
                     LEIEKIGTLKNTIGTKPTPLF"
     gene            2169..2564
                     /locus_tag="PLE39_00435"
     CDS             2169..2564
                     /locus_tag="PLE39_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNE66817.1"
                     /translation="MEQNKIITVRPKETFISKQQLPNFEGISTQTAGTKHLCMHLVII
                     PPGGRAVAHYHNGYETTIYIIKGHAETRYGEKLEHSSINEAGDFLFIPPNLPHQPINL
                     SETEEVVAVVARNDPNEQESVVVYQPQDS"
     gene            2586..3062
                     /locus_tag="PLE39_00440"
     CDS             2586..3062
                     /locus_tag="PLE39_00440"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE66818.1"
                     /translation="MRNTHPRDEDTYTSDVFEVLFNYEVTRSRRYPSPLALLYIEMAP
                     ITLDAELQAQAPAVFAAALNSHLRSVDIPSTYGRAYRVLLPSSNEHGARAVCERLLSV
                     YRNKFEVANGSIAFSLHIGATAHNGGAELSAAELIQKAEEALKQAKLKGPNTYMVI"
     gene            3093..4256
                     /locus_tag="PLE39_00445"
     CDS             3093..4256
                     /locus_tag="PLE39_00445"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNE66819.1"
                     /translation="MPFYHKLGKIPHKRHTQFKKADGKLYREEVMGLEGFSSIQSILY
                     HHFLPPRVKMTEDLGSAKPEYVDFGPIRHRAFRTDQTPLGPDPVSARIPLLGNNDVIL
                     GVSRAKDSMGYFYRNSQAYETWWVHDGSGTLKSQFGNLPFRKGDYIVIPFGVTWQMQL
                     NEECKFFTIENPSQIEPPKRYRNNYGQLLEHAPYCERDIRLPEELETHTERGDFEVRV
                     KVRDRLSRHILDFHPFDVVGWDGYLYPWIFNIEDFEPITGRLHMPPPIHQTFDAHNFV
                     VCSFVPRLFDYHPEGIPAPYNHSNIQSDEVLYYAEGNFMSRKGIDRSDISLHPFGLPH
                     GPQPGMTEASIGKVETKELAVMVDTFHPLNLTKQAAAMEKPEYMSTWLEGDGE"
     gene            4256..5626
                     /locus_tag="PLE39_00450"
     CDS             4256..5626
                     /locus_tag="PLE39_00450"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNE66820.1"
                     /translation="MLNLSTAHIKDKNFRKLSQKVVAEMKRLSIPGVAIGVWHKGREF
                     SDGFGITSIENPLPVTSDTLFQVGSISKTFTGTLIMQLAEQGKVNLDAPVHTYLKDLK
                     LSDKAVEKQVTVRHLLTHTGGWVGDYFNDFGNGDDALARMVKDIAHLPQVMPLGEIWS
                     YNNTGFNIASRLIEVLTGKPYEQAAQEMLLDPLGLKMSFFFPSDLLITHRFVTGHYNE
                     NKKTFVSRPWAIGRAGNGVGGVVSTVKDLLAYARFHMGNGSRIIKRKSLEAMRMPQIS
                     AGGRGEMGITWFINRSGSLTRYSHGGATNGQQALFVFIPEKDFALALLTNNDNGGVLN
                     NNLLGLALEVYFGIKPKLPKPIKVSTNELKEYVGSYRIGTEAFDLKLKNGELIFQHIP
                     LGGFPTPDSPPGPAMPPMRVAFYEKDQTLMLDEPMKNGIGDFIRDKNGRVQFFRIGGR
                     AHKKIK"
     gene            5638..6378
                     /locus_tag="PLE39_00455"
     CDS             5638..6378
                     /locus_tag="PLE39_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66821.1"
                     /translation="MKKFMGLCLVAGIFISACSMPSAAGPSDSEISTAAALTVQAVIN
                     STPLASPTAAGGQQLDPEAATPVVITPTFSQPMASVGDVINCRSGPGTNFERVTQILP
                     TDSVKIVGFYPPNYWVVNTKDGDCWISGEFTTPVGSFAAVPTVTAPPTPEGGGPEAAT
                     FPKNGWTFFCYGPGKADITLSWNDNAENESGYRIIRDGEKVAELAPNTKYFAETIDLN
                     SGESVAYQIQAYNEAGSSDSATANITCP"
     gene            complement(6429..8285)
                     /gene="typA"
                     /locus_tag="PLE39_00460"
     CDS             complement(6429..8285)
                     /gene="typA"
                     /locus_tag="PLE39_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNE66822.1"
                     /translation="MLIERTDIRNIAIIAHVDHGKTTLVDGLLGQSHTFRENQHVEER
                     VMDSNDLERERGITILAKNTAISLIDENGKTVKVNIVDTPGHADFGGEVERVMNMVDG
                     VLLLVDAAEGPMPQTRFVLKKALELGHKAVVVINKVDRKDAEPARVLNETFDLFIELG
                     ASEEQADFPVVYAQATAGRAGLTPEPGPDLKPLFDVILKHIPAPKVDVDAPLQLLVTM
                     LGYDDYRGVTAIGRVFAGTIKAGQKLARMKLDGSILPESARYLYSFKGLNKIEIDEVK
                     AGDIISLAGLEGIAIGETLADPANPVALPTIKVEEPTVRMTFGVNTSPFTGREGTWST
                     SRKLRERLYDELRTNVSLRVEDTESAENFLVSGRGELHLGILIETMRREGYEFQVSRP
                     EVIYHKADDGVLLEPYEEVHVETSNETVGVVVEMLGSRRGKMMEMHDAGQGMTRIVYV
                     VPTRGLLGFRYQFLTSTRGEGVMHTIFSGYDEMAGPISSRTKGSIVAWEAGSTTAYAL
                     KAAEERGQLFVGPGVEVYEGMVVGENARGGDLSVNVCKKKHLTNMRASGGDMEIRLTP
                     PRQMSLDEAIEYLADDELLEVTPENYRIRKRILSTDERGKQTKKAKEQFGEE"
     gene            8543..10327
                     /locus_tag="PLE39_00465"
     CDS             8543..10327
                     /locus_tag="PLE39_00465"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNE66823.1"
                     /translation="MGNPYYITHIIILLISSTIGILLLLRVWSLRSLPGAHGLILTIL
                     CVVEWSLAYAMEIVRLDLTEKVLWGKLQYFGISYAALGMFIFAVNYSGHGARLTSTRK
                     ASLALLASLGFIAALTNDRHHLIWNQIQFTNNIPFGPLELSHGVLFFVVVAFQYALLL
                     AVTGIFLQIAIHGQSLYRAQSKIMLVGMSFPWLANLIYVTGWNPYKGVDFTPIALTFS
                     AIAFSISFLRYRFMDLLPVAHDSVFNAMKDGVIVLDSKDRIADINPMGSFIFQNRGNL
                     LGNEIRVLFPDWNNWISPGHHDEIHQELCLDYNGETATFKLRITPILEQQGEHNGNII
                     ILSNITEQKRFQEQIMEASRLKSQLLANLGHDLRSPLGAIIGYAEMLEDGSFGPMSNE
                     QVKATAEILDGANQLLSFINNLVGQAQIETGKVILREYPFNVEEVIGPLLSTLNFHAH
                     KKGLILIQFVDPDLPKKLVGDQFWLRQIVMNLVHNAVKFTDQGSVTVRFIVQGEDQWA
                     IQVSDTGIGIPPEAQKRVFQAFEQINSMENAKQSGFGLGLSIVAKLTSLMNGKIELKS
                     EAGTGSTFTVILPLKEAVEQEGPTMFVR"
     gene            complement(10330..11070)
                     /locus_tag="PLE39_00470"
     CDS             complement(10330..11070)
                     /locus_tag="PLE39_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNE66824.1"
                     /translation="MRITVVTPTYNEAENLPKLVSALFSLPLDINLLVVDDNSPDGTG
                     RIADELVVKYPGRVDVLHRPGKMGLRSAYLNGFQKVMDSSVDAIVQMDADFSHDPSAL
                     LDMANLLTDNDIVLGSRYVKGGSVDERWPLWRRSLSAFGNYYARTILGLPLHDVTTGY
                     RMWRRETLQQVPFERIHSSGYVFLVEMAYLAHCLEFKIGEAPIYFADRRWGKSKMSIK
                     IQLEAAIRIWQVWWNYRDLKKAGKAGRL"
     gene            complement(11079..11933)
                     /locus_tag="PLE39_00475"
     CDS             complement(11079..11933)
                     /locus_tag="PLE39_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE66825.1"
                     /translation="MADIKSKILIIEDDLDVAEMLNAYFRVQGYDVFTVNWGEDGVRS
                     CQTIHPDLVILDIRLPDIDGYEVARRLRNDRRTSEVPIIFLTEKRDRSDRLQGLEIGA
                     DDYITKPFDVQELRLRVRNALKRVSQGSLTNPVTGLPEGALVDEKLSEVIGKDGVAVL
                     FVSIDNMDSFREAYGFVASDDVLRAISLMIVNTMRETSRPEDFLGHLSTTDFILVIPP
                     SNLAALSEKLRARLEQSVEYFYPIKDREQMVNSRNRLGVKLAEIASLKDRFSDVGQIK
                     AELVRLKK"
     gene            complement(11952..12539)
                     /locus_tag="PLE39_00480"
     CDS             complement(11952..12539)
                     /locus_tag="PLE39_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HNE66826.1"
                     /translation="MITIKSLEEVMGKNGLNAILNLAGLNNYIENYPPDNLDKGFDFA
                     ELSAIGSALEEMYGPRGGRGLALRAGRATFSDALRNFGALAGAADLAFVVLPLQSKLR
                     IGLPAFAKIFSQLSDQQTTVEEKDTEWIWTIHKCPCCWGRTGADKPVCFIAAGLLQES
                     LKWVSGGNEFRVNESKCVAIGDSVCEFIVQKEPIS"
     gene            complement(12598..12969)
                     /locus_tag="PLE39_00485"
     CDS             complement(12598..12969)
                     /locus_tag="PLE39_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNE66827.1"
                     /translation="MSKNRSQQMVDRLRELQASSPDIEASAVVSVDGLSIASALPQGV
                     EEDRVSAMSAAMLSLGERIASELGRGSLEQVYIKGLKGYVVLMSVGDEAVLTALAREQ
                     AKLGLIFLDMRRAAEDLAKLI"
     gene            13124..14260
                     /locus_tag="PLE39_00490"
     CDS             13124..14260
                     /locus_tag="PLE39_00490"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNE66828.1"
                     /translation="MKRITTILFIASLFLATTQSVHAHKQTPPSEPESGGVVCEPAAY
                     LVPPDDCLPIGPSTYLTNLAHLGITFPPRPLPASKPDLSLTQLPYFYFHLDKDYVPVY
                     GAPGEKGPGGQQFPPGFVYVSYIDRVEQNGVYYLMPNGGWIPGKGSRVGEISNFQGLI
                     FSSTPKNSFGWAFEMLPVMKSPGYNAAQTGKQIQPFEVVIVYDVQTMDNEEWYMIGPD
                     QWLEGRKVARVIVNTTIPQGVTSNRWIDVDLAEQTLAVYENNRLIFATVIASGLDPFW
                     TRPGLFQIFEKKETETMRNNDMADFYYLDNVPWTMYFDGARALHGAYWRTRFGYPQSH
                     GCVNLSVGDSHWLFNWAVVGDWVYIHDRTGQTPTDPALYTGGAY"
     gene            14305..14378
                     /locus_tag="PLE39_00495"
     tRNA            14305..14378
                     /locus_tag="PLE39_00495"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14339..14341,aa:Asp,seq:gtc)
     gene            complement(14459..15136)
                     /locus_tag="PLE39_00500"
     CDS             complement(14459..15136)
                     /locus_tag="PLE39_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE66829.1"
                     /translation="MKFRTLRSVVRFVMNIIADVEVVGIEKLPQGNVMIAANHLGRLD
                     TAVLLYILDREDIIMAVAEKYKDHPIFGAIGRAANAIWLNRFEADFAALREILARMQK
                     GGLMVIAPEGTRSKTASMQQGKLGVAFLASKSGYPVIPVALTGTEDGGVVANLKRFRR
                     SKIKAVAGDPFFIEIPKGSGREQAMREATDEIMCRIAVELPESYRGYYENHPRLQELL
                     AERSSTT"
     gene            complement(15141..15806)
                     /locus_tag="PLE39_00505"
     CDS             complement(15141..15806)
                     /locus_tag="PLE39_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE66830.1"
                     /translation="MRSFLRWLIPFVLNSFTTIKIGGYENLPEKGGFVIAVNHLGFLD
                     APMAYYALDNWNLFIPVAEKWEENPLWRFLGRHLNFIFIDRFNPDLKAMREIWRRMED
                     GQTLVIAPEGTRARDEKMARGRPGVAYLASKKGWQIVPVAIAGTEDRIVLSNLKRFRR
                     TEIHLTAGKPFTLPSFPKENRDETLQEYVDEIMCRIAVMLPEKNRGYYAEHPKLKELL
                     SGK"
     gene            complement(15897..16283)
                     /locus_tag="PLE39_00510"
     CDS             complement(15897..16283)
                     /locus_tag="PLE39_00510"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE66831.1"
                     /translation="MESEQRRLARRNFNYYMRVKEEASGKVLGHISDISTGGFRIDSS
                     QSVSLNIDMRLYIDQLGEISSKSYILLKARPMWCRQDQFDPNMFNIGFKLVDATPVDR
                     EIYMKMYEVYGDKTPPKTGELKFPPE"
     gene            complement(16362..17513)
                     /locus_tag="PLE39_00515"
     CDS             complement(16362..17513)
                     /locus_tag="PLE39_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HNE66832.1"
                     /translation="MRKIAVLGIGQTKIDEHWEKSLREIAGEAAFAALQDAGIEKVDS
                     LFVGNMLSPLINGQNQLGSFLADWIGLWKQEAVKIEAACGSGAAAFRAALMAVASGDI
                     ESALVVGVEKMTDKAGRDVTAALATAADADYEVEQGVSFVGINALVMRRYMHEYGWKH
                     EDFAQFSINAHANAMHNPYARLHEKISVEKFEKSSMVATPINLLDASPIGDGAAAMVI
                     VPAEKVARKPRIVVEASAAATDSIAVHSRKDPLFLQAAYESSRRAYEMAGVTPADIDV
                     FELHDAFSIMAALSLEASGFAERGQGVKLALENEISPKGRVPVCTRGGLKARGHPVGA
                     TGVYQLVEVVQQLRGECGQTQVADAKIGMAQNIGGSGASILTHILRNDT"
     gene            17618..18103
                     /locus_tag="PLE39_00520"
     CDS             17618..18103
                     /locus_tag="PLE39_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66833.1"
                     /translation="MLRSYLISLFAAALFLAGCAQSTATAYPNPETGQTAISMPSLQG
                     DWRIKLTHSGGIMGLLRTMEISSDGSYTVTDERSSQTVTKTLGPKELQELTRLISSAQ
                     YIAPSGPNGMVCADCFVYDLEIKGSDSTFTIQLNDISLPDSGMAPVIAHLRDLIDKAL
                     K"
     gene            18106..18618
                     /locus_tag="PLE39_00525"
     CDS             18106..18618
                     /locus_tag="PLE39_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66834.1"
                     /translation="MDQTEWREKFLEAVANCCSEGKEAVEFIHAHKVRIGIKPARKSV
                     GAFWTLEKRAYLNSVYFSPESSLANPNAWVLLIHEVRHLQQGWSTALSIYGELDAWQY
                     QFNTLKKITSAKLPEAVDEILSLPLSMDRETLGRARKLMTQHAGKGYGANLLPLYPIH
                     MEIKYWISPD"
     gene            complement(18920..20083)
                     /locus_tag="PLE39_00530"
     CDS             complement(18920..20083)
                     /locus_tag="PLE39_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HNE66835.1"
                     /translation="MTNFNITPDRRKNFNRTKWTWYEKDILPMWVADMDFPAPKPILD
                     ALHKAVDHGVLGYEIPGAVLQETVAARMDRLYSWKFKPESVVTVTGIVSGFSVATRAF
                     CTPKKGIAIQTPVYNEFHQVKNNVGIPQTDIPLVKNVKGNLISYEIDWDLFEKRVKKA
                     GIFLLCNPHNPLGIIFSRKELTRMAEICIRNKVLIVSDEIHSELMLDGNKFTPMAKIS
                     SEIAQHTITLVAPSKTFNVPGLFCGFAIIPNKSLRERYTTEMEHMRLHVGSMGLHAAR
                     AAFSGKCDPWLAELQTYLTGNRDFLVEYITRNFPGVRTTVPSATYLGWLDFTQTAIDG
                     SPYEFFKDKAKVALSEGKIFGNEGAGHVRLNFGTSRKILEQGLEKMQKAFSAL"
     gene            complement(20153..>20782)
                     /locus_tag="PLE39_00535"
     CDS             complement(20153..>20782)
                     /locus_tag="PLE39_00535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HNE66836.1"
                     /translation="LDYQTVQDYLASLVPSREAELLEMEAYAEKNDFPIIGPACGYYC
                     YQLARMINAKSIFELGSGYGYSTAWFAKALVENGGGVVHHTVWDEELSKRARKHLVKM
                     GFADLVQFHMAEAVETLRQTEGPFDIIFNDIDKHAYPASLPEIKNKLRSGGLLIIDNM
                     LWHGRIFDEKDVSSATMGVQEFTRKITSDRDWIVSLTPMRDGMIVAYKK"
     assembly_gap    20783..20882
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20979..22172
                     /locus_tag="PLE39_00540"
     CDS             20979..22172
                     /locus_tag="PLE39_00540"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNE66837.1"
                     /translation="MKYNFRLFWRTFYRSFFDSKNTPARLTRKRITFLILFYLVWPIG
                     GLIHWFCFALDDILFPAYKNHPIEKPLFILGNLRSGSTFLHRLLSRDTETFTSLTTWD
                     IYLTPSVTQKKFTQLIARLDNKYLGGTLHKLLYKFDAATLGKIKIHPISFFQPEEDEN
                     IHLHIWDGYFVTFLFPFMDEFPNYQHFDEALSPEHKKRIMTFYRSMIQRHMYANGKKY
                     FVAKNPAFSAKIETLAEFFPEARIIYLARNPLDMLPSTISWINYARRQFTDPGEGFFY
                     IEEIVDMTKHWYSHPLQYLDSHPSPRHLIMKYDDLIQRPETVIRAFYEQFGYPDKPGL
                     PVIIDQAVKETLTFNSDHAYSYESMGFTREQIVAMYPEVFKRFNFETREPFIESELMK
                     VERKK"
     gene            complement(22256..23674)
                     /locus_tag="PLE39_00545"
     CDS             complement(22256..23674)
                     /locus_tag="PLE39_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE66838.1"
                     /translation="MLKKKILIPIVIVVVAAATFFAFVAAGQNSLKEADAHFTAQNYA
                     EAAKAYERSARLLFWQTGLWEKAGIASAKGGDTAQAIIYLDQAGDTLSEEGWGWLGSS
                     YLSLGEVDSAIEVLQRGLQTHEAPMLHGLLAFAYRQKKDWPAERAALEAQLRLDPNGG
                     DVYARYRLGLLLSVLEPEQALTDLMLASSLNPEFDPAVQTLRTALNLSATQPDASEQM
                     IHIGRALGLVQEWDLAVAAFEKAIAFNAENAEAWAWLGEAKQQSAQSDAENIGLAELD
                     QAVLLDHESVTVRALRGLYWRRQEKYPQMLAEYLLAAEYDPTNPAWQAEIGNAYIKRG
                     DLVSALAAYQHATELAPDDPTYWRLLAVFCAENTIHMEDIGLPAAEKAVELAPNDPLT
                     LDALGWAYLSSGRFANAENIFVDLITRFPDHLQAHIHLALTYLSMGDRDAAFEKLNEV
                     KNMDPAGPYGQEAGRLLQLYFP"
     gene            23738..26095
                     /locus_tag="PLE39_00550"
     CDS             23738..26095
                     /locus_tag="PLE39_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE66839.1"
                     /translation="MSPFTSQIFELLTVPINNLLYYIVLSYAVASAMLSAFIHWRASE
                     FPQARRALIGLGVLLAAQVTMFLFSGLGWQGLIDPKILPPMDRAFIIFGIIWITWLYA
                     FPEPSRPADAAAALLSVLVLTALGLSLLAWQPQITTITYNQTSDDWFWQIGSLAVGLI
                     GIAIMFVRKPDGMWNGIVLLGLGILGHIGHLAFPIDGNYSGVVRIAYMAAYPILLTLP
                     LRFSTPSSANESPARQITQKQDENLGERRRYSTDPKTFHAMLALAAETNPTKVSQAIT
                     RAIAQTMLSDLCFMIYINDNSNQMSIAGGYDLIREDALEGGTLNKGSIPMLANSLQRG
                     RPLRMPSSSTSADIKGLSDILGLTNPGHLLSVPILTAEKESLGGILLLSPYSDRTWTA
                     EDQAYLSNIATSLVPIIQRGQKVNRMEAQSSEAIRRSADLEHRVQELTEELHNAQTEI
                     QKAGSVEMASLLQAQEESQRIIEQLQLENAELRTGKSLQTGAAPASFASGQIEQELKT
                     TLQEVARLQNQLMEANMKTRELEKGHVTVKSTEQAEVVASIAQELRQPLSSIVGYTDL
                     LLGESVGILGALQRKFVERVKSSTERIRSLTDDMIQITTLEAELNDLNPEAVDLNAII
                     DNAMSYTSTQVREKNISMHLELPKQLAPIHADREALQQILIHLLQNAGAASPFEGTVR
                     LKVQTRNEENTEFILIQVTDSGGGIPAKELPRVFTRLYRADNVLIQGVGDTGVGLSIA
                     KTLTEAQHGRIWVESEQDVGSTFSVLLPIAGSAETNPKTKPKGKK"
     gene            26104..26925
                     /locus_tag="PLE39_00555"
     CDS             26104..26925
                     /locus_tag="PLE39_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE66840.1"
                     /translation="MRDLRNALVVAMISIGLTVGSLSISLVEFAPQAVPSATNPLQVV
                     PPLLTATPTVVPSMTATLGLESPTPSPTSTFTATPPPPTTCPPPTGWTQLVVQAGQTL
                     DTIAQQYRTSKENLRAGNCLISDNLVAGSIMYAPAVMTSTVPACVPGAAGWKKSYTVV
                     SGDTLYAIATNHSTTAGLLKSVNCLSLDDIYPGNVLWVPDVPTRTPNPTPLPGSTVTP
                     IPTQLLTETALPYTATTLPSVTPTEVPATPTPTTPATDTPTTAPTLTASPTPFPP"
     gene            26979..28490
                     /locus_tag="PLE39_00560"
     CDS             26979..28490
                     /locus_tag="PLE39_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNE66841.1"
                     /translation="MKSNSAFSTIALLAFLLSACGTPAEAVVQTPLPFVLITSAPNPS
                     PTPTPFQPSLNTPTQSAILSAAVDTSTPVPTVILPTETPSPTLIPPTESTATVDFNQL
                     FPTVAAPPVVEVVAVTPTALPPLTDNETVNFLLIGSDKRPGSSYRTDTLVIAIVWPKE
                     GQVSLISIPRDLWIYIPTVGMQRINTAYQSGEIGGYAGGGAGLLKDTITYNLGIRIDH
                     TAMVEFDGFRRIVDTLGGIEVPVACAYTDWRLIDPSLDMYDENNWWLYTVGPGQVHMD
                     GDLALWYARSRSKSSDFDRGRRQQEVLRTIFQKALQTDTFSKIPQLYEDFSSTILTDM
                     GLADMLLMSPYAVNFTNANIRGYFIRPPYVTSWTTPAGAAVLLPNETELNQMLLEATT
                     LSTFAEQRETITVEVQNGSPFDTMESLAASRLNYAGYQTILSNADRRDYANSVLVDFT
                     PGQDATQREAIIATLNLYTANVISLPDANSTVQYRVILGSDYQACFQPQDLSH"
     gene            28624..29523
                     /locus_tag="PLE39_00565"
     CDS             28624..29523
                     /locus_tag="PLE39_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNE66842.1"
                     /translation="MAKTPDSAHISAKTLLPAAIQGWEMFMLDQGRSTNTVKAFLSDV
                     RLLTQFLPPDQSIGSITTEDLNRFFTWMEKDRGIPCSPKTLSRRITSVKSFFRWLHQH
                     GSLVLDPAEKVLQRSAISPLPQVLTDDEYEAVLLAADRLRRASKPDARSYTLVYLLLT
                     TGIKKSECLGIHINHIDLEAKNGPHVFIRYASPANRYKERKIQLAEDWLEAYKEYLAQ
                     YQPVDQVFAWSPRRLEYLLEDIGNEAGLTKHLSFDMCRWTCALRDYQSGMEADNIRQK
                     LGVSKIQWRELFIKIRQLNGEIK"
     gene            complement(29585..31030)
                     /gene="tilS"
                     /locus_tag="PLE39_00570"
     CDS             complement(29585..31030)
                     /gene="tilS"
                     /locus_tag="PLE39_00570"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNE66843.1"
                     /translation="MMLENMDATLQDECGLVKDRPIIVGVSGGPDSLCLMETLRQAGY
                     SIIIAHFNHQLRPEAGQDAHMVEKIAARLMLGCIIDGADVRAYADQQKLSLEEAARNL
                     RYHFMFKLARERNAQAVAVGHTADDQVETILMHFLRGSGISGLKGMSHRSIIKTFDPK
                     IPIVRPLLNTWREETTIFCAANGLRPHYDSSNDSINFQRNRIRHLLIPSLESYNPKFR
                     DVVLRMSQSLKSDYSLLMETLENAWQEVVTSVEEDTIAFDADLLANRSIGLQRNLVKH
                     AMQILRPKLDVNFSALERATDLIKDRSHSSSVDLKGGLRMFREAGVLYVCTQNSKLPF
                     NLWPQMPSTTPVQVAIPGQVELAGGWRFSCERWHIPTLVREQAERNEDPFQIWLDAER
                     VPEMLELRVRHQGDTFEPLGLEGHSQKLSDFFVNEKMPQRARDHWPLLCAGDQIIWVP
                     GYRPAHSYRLTQSTKTAIYFSVSGPLEKITE"
     gene            complement(31100..31504)
                     /gene="mce"
                     /locus_tag="PLE39_00575"
     CDS             complement(31100..31504)
                     /gene="mce"
                     /locus_tag="PLE39_00575"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNE66844.1"
                     /translation="MPKIKAVNHVAVVVDDMEKSLLFWRDALGIQLHELRDVPAEKSQ
                     VAFLPVAGAEVELVMPTSDDSGIAKYLAKRGPGMHHLCLEVDDIEGMMSQLKAKNIRL
                     INEEPRTAVDGKKYAFIHPESTGGVLVELYQI"
     gene            complement(31527..32132)
                     /locus_tag="PLE39_00580"
     CDS             complement(31527..32132)
                     /locus_tag="PLE39_00580"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNE66845.1"
                     /translation="MEIRYLALGDSYTIGESVRESERWPNQLAAKLEEQGIHAETTII
                     ARTGWTVDELWEGIQVTPPEGRYDLVTLLIGVNDQYRGYPLDGYREDFRFMLGKAIEY
                     AGGDPQHVIVLSIPDWGFTPFAATRETEPISLQIDGFNAVNLEETKSAGAHYVDVTEI
                     SRMAMDDFELLAPDRLHPSGKMYSMWVEKVISVALEIVNRK"
     gene            complement(32289..33236)
                     /locus_tag="PLE39_00585"
     CDS             complement(32289..33236)
                     /locus_tag="PLE39_00585"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HNE66846.1"
                     /translation="MQAQANWESQLIALAGHFPHTSARPFFSYWAGDAQLHAAYKKAA
                     KITSEHSKSFYLASGLLPEEKRSAVRALYAFCRTVDDIVDEVELKKDRDFELDYWRNI
                     VKTASSPTDDPIASAWADTLTRYHIPHHYALQLIDGVARDLSQTRYQTFEDLATYCYG
                     VASTVGLMSMYIVGFKGHDAVPYAIKLGVALQMTNILRDVGEDYRNGRIYLPREELVF
                     YGIQEADIAEGKVTDRWRQFMKFQIDRTRQLYEESWEGVKMLEREGQLAIGAASTFYC
                     GILEDIEKHDYNVFNRRASLSAMGKISRIPSLWLKVRSL"
     gene            33415..34950
                     /gene="crtI"
                     /locus_tag="PLE39_00590"
     CDS             33415..34950
                     /gene="crtI"
                     /locus_tag="PLE39_00590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNE66847.1"
                     /translation="MRKQTAVVIGAGIGGIATAARLAKNGYDVTVVEKNDTPGGRCNQ
                     IIQDGHRFDIGPTLFLMPEVWEETFAALGEKLSDHLDLRRIDPTYKVRFDDGLQLELT
                     SNIGDMQTQLEKVEKTAFTGFLNYIAEGSKHYKISLEKFVGRNFYNIFEYFSLSNLPL
                     IFQLKALGKHYTNTGRFFKDERLKAAFTFQNMYLGLSPYDAPATYSLLQYTELAEGVW
                     YPMGGMYAGIQALTRIAEKLGVKFIYDAPVKKIKTGKSKVDGVILEDGRELTADIFVG
                     NADLPYIYDQLLPDRAEAKKLIDEKLYTCSTIMFYWGVDKEYSQIAHHNVFLGGEYKA
                     SFEKIFNEHTLPEVPSFYVHAPKRTDPAAAPEGQDTLYVLVPVGHLDARSAQDWDALV
                     ARARRYVLDRLAKEMDAADLEAHIKFEIVYQPKTWKERFNLVNGSAFGLSHNFWQVGY
                     LRPHNRHKTYKNMYFAGASTHPGTGLPIVLLSARLTTERILKEFTGQQVEGYKLEKAV
                     AQG"
     gene            35028..36350
                     /locus_tag="PLE39_00595"
     CDS             35028..36350
                     /locus_tag="PLE39_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNE66848.1"
                     /translation="MSISKNPAFNLKVVLQETGIAADTLRAWERRYGIPMPQRTAGGH
                     RLYSQYDIETIKWLMARQAEGLSISRAVDLWNEHNASGVDPLAGFIPTTNIAPLATPA
                     ITLSADTSLDSMRAQWIDACLNFSEATAEQVLNQGFSMFPVEVICTEVLQRGMSEIGG
                     LWYENRASVQQEHFASGLAMRRLDALISASPAPSLKQTVLVGCPPNEWHTFTPLLLCL
                     LLRRRGFGVIYLGANVPAEQFEETVSSVHANMVVLVAQTLITAAALHSTALSLVNMKI
                     PVGFGGRIFNIHPALKEYIPGHYLGNTVTASVERIEQLLKGNQSKVQAKSATEAHHVA
                     LEHFNSQRLHIESSIKEMLPPFVAKDIDTGIHFLGDNIAAALQLGDMEHVTAELEWLK
                     VLMQSHQRPASELADFMHNYATAVDKHINGRGEPIKVWLNEQARLTQL"
     gene            36347..36919
                     /locus_tag="PLE39_00600"
     CDS             36347..36919
                     /locus_tag="PLE39_00600"
                     /inference="COORDINATES: protein motif:HMM:NF021577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DICT sensory domain-containing protein"
                     /protein_id="HNE66849.1"
                     /translation="MTKQDILSTFYHKLLPLFPPRTEGYLNRLTAQDLQHSNFRYIFS
                     VQSMIEISHMIEDSVINTPGVAETHVSFQYFSRIPAQLEQYRKVSEASTGLWLYGVPD
                     APLPQLSRTIGVSTDGTPLETYWFVVAYGPGLYATLLAEEITPEEGQRMYEGFYTFEP
                     ETAFQVVSLLHQMFPMQVPAPIAPQDQEHA"
     gene            36921..37757
                     /locus_tag="PLE39_00605"
     CDS             36921..37757
                     /locus_tag="PLE39_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNE66850.1"
                     /translation="MNIRIVTDSTCDLPDEVTKQQAITVIPLNINIGNDTFQDGIDLT
                     RTEFYARLPEYSPSPKTAAPGPDVFKKEYERLADEGARSILSIHISETLSATINSART
                     AAEQFTRIPVIVLDSSQLSLGTGFLVEKAAIMGAAGETMEMIVSTLQKMMKRTYVFAS
                     LKTLEYLRRSGRMNFALASFGELLQIKPLLHMHLGKATAHRTRTQKRATERLLSWLNE
                     YAPYEKLAVLHAGAQDEAEALREKVRSFLPNDDVSIIQITPVLGAHLGIGALGFACIS
                     KE"
     gene            37762..38088
                     /locus_tag="PLE39_00610"
     CDS             37762..38088
                     /locus_tag="PLE39_00610"
                     /inference="COORDINATES: protein motif:HMM:NF018991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1475 family protein"
                     /protein_id="HNE66851.1"
                     /translation="MKIAKLISILGILAMTAALVNGFTRGDFFGEGGQLAAMPWGIVS
                     LVDLYTGFTLFSGWIIFREKSLPVAIAWTVAMMVLGFFAGSLYAFIALQTSGNDWRKF
                     WLGKHA"
     gene            38081..38779
                     /locus_tag="PLE39_00615"
     CDS             38081..38779
                     /locus_tag="PLE39_00615"
                     /inference="COORDINATES: protein motif:HMM:NF022805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HNE66852.1"
                     /translation="MPEKTLAPQGKGRELLEELQTVFAGRNSFMDAILPPILFLAVNG
                     LVNFQAAMWSALALAIFFAAFRIVRKQSLMYALAGVGSVAAAIGIAWFLGKSEGFFLP
                     GLISGSVTLFLTILSLVIRRPMVAWTSYLARRWPLEWYWHPLVMPAYSEVTFAWAVFF
                     AIRLLYQFVFFQDADVNSLAVLNFITGWPATVLLLVVSYLYGTWRLAQLRGPSVQEFL
                     DDSPAPWQGQRRGF"
     gene            38806..39672
                     /locus_tag="PLE39_00620"
     CDS             38806..39672
                     /locus_tag="PLE39_00620"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNE66853.1"
                     /translation="MKTTDRNALIAFPILILIGALVALAGSQGGASIAGVPLFAASVG
                     LAFLIQWLVFIPAYLMQTEKFFDLTGSLTYISVTIIAVIFSGTMDGRSVLLAALVVIW
                     AVRLGSFLFNRIRKAGKDDRFDEIKPSFIRFLNVWTIQGLWVTFTMAAALVAITTSAK
                     KGLDAFAIIGLAIWVFGFAFEVVADSQKSRFNANPANKGRFIQTGLWARSRHPNYFGE
                     IVLWLGIAVIAIPVLQGWQWVALISPLFVTLLLTRVSGVPLLEKKADQKWGGQEDYEA
                     YKKKTPVLIPRL"
     gene            39691..40038
                     /locus_tag="PLE39_00625"
     CDS             39691..40038
                     /locus_tag="PLE39_00625"
                     /inference="COORDINATES: protein motif:HMM:NF025607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HNE66854.1"
                     /translation="MIIQILQIIAAIATILTGLVSLFKPESVTGFTGLRPEGGRGITE
                     IRAVLGGFFVALGAAPLMLNSPVAYTMLGMAYLVVGVVRGVSMWVDKSVTQSNIISLV
                     VEIIFGVILVVPV"
     gene            40035..40517
                     /locus_tag="PLE39_00630"
     CDS             40035..40517
                     /locus_tag="PLE39_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:40164..40166,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HNE66855.1"
                     /translation="MSRAELNKPAPNFTLTDFNGNEISLTDFHLKKNVLLVFNRGFFU
                     PFCRRHMAQLRQDYNKFTEQDTVILVVGPENSLAFSLYWKTNSLPFIGLPDPSHTVLK
                     RYGQEVNLFKFGRMPAQVAIDKNGIARFVHYGHDARDIPTNEEILELLESMNKESITS
                     "
     gene            40527..41747
                     /locus_tag="PLE39_00635"
     CDS             40527..41747
                     /locus_tag="PLE39_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE66856.1"
                     /translation="MNNKRLFSIILVVFIDLLGFSLILPLLPYYAETFNANETITGIL
                     IASYAVMQLIGAPLLGRLSDRFGRRPVLLISVFGTFIGFLLLGFANALWMLFASRILD
                     GLTGGNLSVAQAYISDVTDEKSRSKGLGMVGAAFGLGFIIGPVTGGLLSQWGYAVPAF
                     VAAGISFLNLILIYAWLPESLTEEKRNQMTEKRPAVTLQALIVAFKRPFTGSLLITRF
                     FYGLAFSIFQTIFSLYALAKFDLTARDTGFVLTYVGVLAVIVQGFLVGRLTTQFREDL
                     LITVSVVLMGISLLGWALAPSVLWLYIIMTPTAISGGLLNTLLSSTLTKAVAPQEIGG
                     ILGLSAAVESATRIIAPLLGGVLLQQVGTWAPGAFGAVVMTGVSLYVFTTIYNHPIVA
                     TLKQGKNAPVPASD"
     gene            41783..42403
                     /locus_tag="PLE39_00640"
     CDS             41783..42403
                     /locus_tag="PLE39_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66857.1"
                     /translation="MTDKTTRYELPPSGITLLGYVVRRMHKTRWLLPVPDLLVQGKTD
                     MALFNVAMYAVASSTSFGRAYYQPQFNSAGAEITETDPLTGANVTAKLVATSPHYFIT
                     YTAAMPNGDLFTGSEEITGTTVGLRGLGMPAPSKFTFTSGGYTAELSGVLTSELALSL
                     IGRTRIRAYGFLNFKDNAGNTGKLNLDRSRKIKIDINDKAMEPKFI"
     gene            42400..43740
                     /locus_tag="PLE39_00645"
     CDS             42400..43740
                     /locus_tag="PLE39_00645"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNE66858.1"
                     /translation="MTSIWWIRRDLRLTDNLALHSALKTGDVIPVFIIDPAFSSQSPR
                     RRSFLYEGLHTLDQDLKKRGSYLVLRTGRPVDVLHQLMQETKAQAVFAEEDFTPYAHK
                     RDTHVAQSVPLHLLPGQTAQHPSRVHKQDGKPYTVYTPYSKTWKAMLEEIELVPAPDV
                     IPTPAGISTEPIPAFGVNPLFPAGEQEALTRLDEFAFKRIHAYDEARNRMDLDGTSSL
                     SPYLRFGMLGLRQAVRLAKQASAESRGAEIWLNELIWREFYIQILYHFPYVSHREFNP
                     ALMGIRWRNNIEEFECWKEGRTGVPIVDAAMRQLKETGWMHNRARMIVASFLVKDLLI
                     DWRWGEAWFMECLLDGDPAANNGGWQWTAGTGTDAAPYFRIFNPVLQSAKFDPLGEYI
                     RKWVHELRGLNEKEIHAPWEHGVKISGYPEKPIVDRALVKERTLQAYKQVKSER"
     gene            43796..45049
                     /locus_tag="PLE39_00650"
     CDS             43796..45049
                     /locus_tag="PLE39_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNE66859.1"
                     /translation="MNSKITPELGRAAMRALFETRSPLGPLKVMAQHVGRFFQIPLPG
                     FRPYVVFGPEAARKVLITERDKVLWRNTDPVTDLLGRGVLITDGKEHDHYRELMEPPL
                     HPSNLSGYTQMMLEKTDQVTSHWKSGDTVDMLVESRKIALLIIMQTLFSKDVWADLPR
                     IWKPILKAIDFISPGAWIIWRNMPRFGYRKPLRELDDYLFEIIEERRKTKQAPHDLLQ
                     HLIDAGLTDKVIRDQMLTMMIAGHDTSTALLAWTFALLGQHPETLKHVLAEVHSKDRS
                     PLLDHVIKESLRLYPPIHIGNRRVAKTMEFAEGTVPAGERMFYSIYLTHRDPSIWENA
                     EEFQPERFAHGRKTPPMSYIPFGGGPRACIGAAFGQAEARIVLTRLLQTFSFEPMNPD
                     KIHAHMGATLEPRPGVLMKVQPLIP"
     gene            45046..45837
                     /locus_tag="PLE39_00655"
     CDS             45046..45837
                     /locus_tag="PLE39_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HNE66860.1"
                     /translation="MTYFGFLLRFLVIPILIFLAITWWDNKNNRPTLGFTNGRAVWIA
                     ILVHILLAVTYTTPWDNYLVATGVWFYNPNLVTGIVFGYVPIEEYTFFVLETILSGLW
                     WWFLARRISEPGGFKASMPARVTSSIVLFIAWVIFTAIFFSNNEPLTYLSITLFWAFP
                     AILPQLLYGADILWHHRTLVLLGILIPGTYLSLMDIIALNETTWSISKAQTTGILFFG
                     ILPLEEVVFFFITNILITFGMTLLLSNIGRQRFSDWKLNNYKGLP"
     gene            45834..46214
                     /locus_tag="PLE39_00660"
     CDS             45834..46214
                     /locus_tag="PLE39_00660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HNE66861.1"
                     /translation="MKLEQFKKQQFLNIESFRKNGQAVKTPVWFVQDGETLLVWTQAG
                     SGKAKRIRRDGKIRVAPCTGSGELLGEWAPAYAKTNESVETVKHTTTLMKKKYGLFFT
                     IFGFIGRMRGGSNYTTISIQLQNQ"
     gene            complement(46296..47651)
                     /locus_tag="PLE39_00665"
     CDS             complement(46296..47651)
                     /locus_tag="PLE39_00665"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HNE66862.1"
                     /translation="MPQTTIACPRCKQMIPANVEQLFDVTHEPAAKQRLLSGQSNHAR
                     CQYCGYEGRLATPVVYHDNDKELLLTFFPFELGLPVNEQEKLIGPLIKQVTERLPLEK
                     RKAYLLKPQANLTYESMIETILNKDGISSEMIKTQQERVMLIEKLIRMTSADARLEMI
                     RQNENAIDEQFFSLFSRIAQNAMQSGQETIARALIDLQRQLLDETAFGRQLRDSVAEL
                     EAAQKTLQDAGQGLTREKLLDFVLESQNDSRIRAYVSLARGGMDYVFFQLLTEKIEKA
                     AGEEKSRMEGIRDKVLGFVSDVDKQMEARYKQAQDFVENLLAQEDIERATQENLENFT
                     QDAVEVVNQLLRQASEKNDYARMGKLQKMVQVLQAASTPPEVAFIEHLLQAPDEATLD
                     AMLSQNEEMVNQQFIDALSGLVAQMDSQGADNPEAKAMGEKLTEIYRVALKRVMKKNM
                     G"
     gene            complement(47755..49563)
                     /gene="pepF"
                     /locus_tag="PLE39_00670"
     CDS             complement(47755..49563)
                     /gene="pepF"
                     /locus_tag="PLE39_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HNE66863.1"
                     /translation="MATSIPPRSKIDKKYKWNSESVFPSDKAWEKEVEQIIADIPEVK
                     KHQGGLGESAAALLAGFQAFEAILIRAQKAYMYASFSYSVNTTDQTAAGMRAKAGGMY
                     GQVASAVSFLQPELLEIGKDKLDQFFQQEEKLGVYKFYVENLFRKQAHVRSAEVEEVL
                     GMVSEPLGGFYNSTSMLTNADFKFKSIKDSAGKSFDVMQGNYDTQLMGLVDRKARKAA
                     YITYMDQYVAHKNTLASNLAHNIQSNVFNMRVRKHESTLSASLFDLNISTDVFHNLID
                     TFKKNIPVWHRYFNIRRKALGLKKFAYYDIWAPIEKKKVKIPYDKAVDMICEGLAPLG
                     KEYTAQVRRACTTERWVDVYPNQGKGHGAFSSGSYGTHPFIMMSYTDEMVSMSTLAHE
                     LGHSMHTYYTFKNQQFINTDYSLFVAEVASNFNQALLRGHLLNTVKDKNFQVALILEA
                     VGSNMFRYFFQMPTLARFELETHQRAERGEPLTADSMIELMADLFAEGFGPHFDMDRE
                     RVGMTWATFPHLFADYYVYAYATGISGAHALAGRILRKEPNAVEDYLGFLKAGNSDYS
                     LNVLKNAGVDLTTPKPVEETFAVMEGYINRLEELLG"
     gene            complement(49630..51195)
                     /locus_tag="PLE39_00675"
     CDS             complement(49630..51195)
                     /locus_tag="PLE39_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE66864.1"
                     /translation="MSIIPSMLPDFRVRQRDYLLEISRLITEELDLEKLLARILRVSI
                     EMLAGQAGLIALKQADGWRVSAAHGIAPAFLSYLTPLLAEEKVRELDVRELNRMLKNL
                     TYTASMGLLNGTGLPLAAHGQVIGVIFIFRNYADLFTPDDRVLLQSFADQAAIAVYNA
                     QLYGQVSYEKKRLDALLESAADGILILDANLDIERCNGAFEKLFGQPREEITGKNHAD
                     IIKWKKTPQGITLEDSVAEGWPLTPNATLYVEGDLAKPAPPPLPVGITYAPLLSEEGK
                     LRNVILSVRDITHFRTADEIKATFISIVSHELRTPVALIKGYASTLRRDDARWDKHTI
                     NDSLAVIEEEADRLSKMVDDLLDASRLQAGGLSLNQADVAVPSLALRVSERFASQSPK
                     HSIVTDFPSNFPVILGDENRLEQVISNLVSNSLKYAPKGEIKISGSVLPEQVIVCVSD
                     EGPGIEAKDLPHIFDRFYRSTNAVKQTKGAGLGLYLARAIVEAHGGHIWADAVTGSAK
                     SESGARICFSLPR"
     gene            51422..59275
                     /locus_tag="PLE39_00680"
     CDS             51422..59275
                     /locus_tag="PLE39_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HNE66865.1"
                     /translation="MRIFSIQAFNQSKSAMVRLFVFVLLLASMLAQEHVPGVQAQAGT
                     PQLILTKTIEGGATTASLGDTIRYRIRFECSSLTTSCGQMEITDVLPAGLTYAPPPAS
                     SAPTGFTITEAPAGTITITKDDNNLLDGTQYDVVIAATIDYDLRPLPATINNTVNGRV
                     DPPGAVSWQNATPASAPPITVSNVGEYWDLTKTLYSPSVNPTINTDVTYRIQLCPTTP
                     PPGTSNVALENITITDTLPTGATFVSASDAGTEAAGVVTWPLIAGPVFPPTCVTRYVT
                     IRYNSPTFAVGNNVTNTANASGTYTDSGGGTIGPISIATDPITHPIATVLEVPTYSKN
                     DQGDPVGITGTARFVLTLNTNGTNSPANDLILIDTLPPELQVTSVTSGQWNAAFNYVR
                     AYVEYSTNYGSSYTAFPGQPISYNSNATYTAPAANITNVRWRFEYDPDAAAPFAFTQA
                     GLPFAWAFTTSPQIRVTPRATATTADAPSGVAMPAAVAGTRYENCLQTSRTSNAGAPV
                     TDPCDIEGMTVQGTLASLRASKAETRGTSWDADNDPLINTFTSDSTLLPGDTLRYTIT
                     VSVTERSSQAMIDPTILDTIPADLIFVRNGTASVNGTGCPALASQPTFTQAGTQLKWD
                     FTGFSLEHQPSGLGSCNLVVEFYARIPAGQTPGTRTNDLILVTDSDDIVCENGTEIVD
                     TTDIDSDANNTEPACQNPDTYVVERSAALRGEKWIRSVDPENSVVVDSTTFQPSASCP
                     DGGTVGLAGGGSNPFTRFPCISQAFPEGALSIGQRVPPPLNSALDDFEYNLRIYNDGN
                     VEMLSYALYDIFPYYGDTGSGGTLANSPRLSEFRPILRGPVEFISGPSLSSTDFTIEY
                     NNTTNPCRPEVFNQPAGTPNIPAGCNNTWSSTWSADARSFRILLNSPAVIPPSATTSE
                     VRFGIPMYIPKDAPTVGVFDNNDPLSLEIAWNSFSHVGSYDSDPNVPVVIQDLLASEP
                     RKVGITIPERMSVGNRVWRDSDNSGHIDAPDDIAPGIAGVTVNLYRDVDDDGVPDGPA
                     IATTTTDSLGYYLFSNIPHDLADVNNNRYLIGIPSSNFGSGQPLFNLRSSTGVFATTD
                     DSDDNGIDPVTAGQEVFSASFILQPTTEVTGETDLSSNDLDGPPGERRGTNAERDYNS
                     DLAIDFGFFGGTDVPFSIGNHVWFDNGRTGVSTFNYALNNDGIRQATELPVVGLDIRL
                     YRDGNGNNTPEPAEMIRTDITDANGFYLFDNLDPGPYIVEIPASEFGAGQPLEGWNNS
                     QVIGTETTGVPGNTNIPNVDSDDNGVDSSTPHVDGIFSGVIVLTRGTNETTGETHISN
                     QPDPDGNAATPNLGINPTEWDGPASRGRYGESDTTSNLTIDFGFFEVYSLGNVVWVDD
                     GTGGGTADDGLRNGTEPGLNGVTVNLYLDANNDGRPDTVTPKATTTTANGGYYRFDNL
                     AADTYLVEIVLPAGYSSSPIDAGDPDTDEDDFDDNGVVPATISTVLSNPVTLGRGTDE
                     PTVDSDAATANNPEPGEAPNHHSNRTVDFALTLGYSLGNRVWVDLDDSGTINAADGVT
                     PGLDNMTVNLYRSSDPSTIIATTLTAGGGYYRFDNLLAGDYIVEVVSPVGYTSSSVDA
                     NDPNTDNDSDDNGVIISATTVRSNPVSLGGIEPSTDDDPLPNPAATGEAPNARSNRTV
                     DFGFFFVPYSLGNRVWDDSDGAGGTPDNGVRDGAEPGIDGVTVNLYRDSDNNNTPDGA
                     IIATDTTSGGGFYRFDNLAADTYIVEVIPPAGYASTLDAGDPDTDLDDDDDNGVVFAG
                     ANIRSNPVRLGPPEPSEPTLENNPAVNPETADGEAADDHSNRTVDFGFVLGYSLGNRV
                     WDDSNGAGGTPENGLRDGTEPGMDGVTVNLYRDSNDDSNPDGGIIMSTSTSGGGFYRF
                     DNLMADTYIVEVVIPTGYTSTLDAGDPDTDIDDDDNGVVIVGSNVRANPVTLGPAAVP
                     EPTLENNPATNPVAGESPDARSNRTVDFGFIRSSTPPPPTGSGIQIVKTASASVVKPG
                     DTVVYSYVVSNTGTTPLRNVIVTDDKCSPVTYISGDVNANNILETTEKWNYTCSAILS
                     ETTTNIATVTGKDPTGGDVKDTDTVTVTVDTNGFSKIITGTSETFTNGMDVAIGEIVT
                     YKITMSLPAGLPYNNVVVTDRMDKGLAFVDCVSATLGGVDILNQICPPLVSAIKDPID
                     PASNPANPGRQAQFNIGNVSASTKPTALVITYRAIVLDVIENQEKDTLNNKATLTWDG
                     GSLTASAPEVVILEPDLDIEKAANPTTVTAGSSVEFTIFITHTSLSLTDAFDVVVTDV
                     LPKGLEYVACTPITYEGVVPTKQPDPCSIPNGDTLQFEWDVLPLGGNSVIKFFARYDG
                     SQVSVMNSAAVAWTSLPIDYINGKPVQHSGYNDTSTERWYDPNDLVNIYQISDNVWIN
                     PPTITEPIEEEELPGSLPATGFAPNVITALPQQPANKAYTTTDIWVEIPSIGVNMPIV
                     GVPLVDGDWDLSWLWNEAGWLNGTAFPGWDGNSALTGHITLPNGRPGPFAMLGNLKWG
                     DKIIVHAYGTAYTYEVRETRTVSPDNMTILKHEEESWLTLITCKTYDKATNTYARRTA
                     VRAVLISTTADKQTKEAKSGR"
     gene            complement(59347..60669)
                     /locus_tag="PLE39_00685"
     CDS             complement(59347..60669)
                     /locus_tag="PLE39_00685"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HNE66866.1"
                     /translation="MTNPNLPGPKAKAMIERDRKVISPSYPRGYPFVMDHGKGTEVWD
                     VDGNRFLDFMGGIAVVSTGYSHPKVVKAIQEQAEKFIHISSDFYHENWIRLGEKLSDI
                     APFKEDALTFMTNSGTEAVEAAIKLARYHTKRTNFIGFTGAFHGRTMGAVTFTASKAK
                     YHGGFYPLMNGVTHAPFPNPYRPVLERRKGEDYGEAVVRYIEEEILAQILPPKDVAGI
                     LVETIQGEGGYIIPPEGFYPALRKLCDKHGILMILDEVQSGMGRTGKWWAIEHFGVEP
                     DMITSAKGIASGMPLGACIARKSVMDWEIGTHGNTYGGNPISCAASLATIDLIENGFM
                     KNAEEVGSYALDALEEIKSRHPSIGDVRGKGLMIGVEFVKDRETKEPDHDLTERVVDL
                     GFERGLLMLSCGKSVIRIAPPLSISKSEMEEGLKIFEDALTVAEKEMK"
     gene            61089..62411
                     /locus_tag="PLE39_00690"
     CDS             61089..62411
                     /locus_tag="PLE39_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HNE66867.1"
                     /translation="MRKNFLADFWQALRDIPRWLRPGLGIKRWLTLVLIGITLLGLGL
                     AVILIDLYRTDSENPILLTFLSYASLRFIPRVWRAVIFGGLGVGVVIYGIVQLNRSLL
                     TPFVRPGADVYGQLTDFRRRERGPRIVTIGGGHGLATLLRGLKTHTRNLTAVVTVADD
                     GGSSGRLRESHGILPPGDIRNCLAALSNDEAMLTQLFQYRFSGSPGLEGHSFGNLFIT
                     AMADITGSFEEAVAESGRVLSVNGRVLPSTLHDVKLVASMELPHSLNEIRVEGESKIP
                     TMAGRVRRVWLEPDNAPAFPPVIKSILNADVIVVGPGSLFTSILPNLLVNDLLAAMHA
                     SRAIKIYVCNIATQTGETDSYTCFDHVRALEGHVGEDLFDVILCNDNYRGIISADSKW
                     VLADEKILSDSRTYCADLVDDDHPWRHDSEKLAREIIQIFSEKTGPLV"
     gene            62599..63657
                     /gene="gap"
                     /locus_tag="PLE39_00695"
     CDS             62599..63657
                     /gene="gap"
                     /locus_tag="PLE39_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNE66868.1"
                     /translation="MTVKIGINGFGRIGRQVLKAIRDMYPNELEVVAFNDIGDTKTMA
                     HLLKYDSTYGRFNGSVEIADDGLLIDGKKVKVFKETDPAAIKWSELGIDIVIESTGLF
                     TIKDDGVNKKGKTVKGAVNHITSGGAKKVIISAPAEGEDLTVVLGVNEDKYDPAKHHV
                     VSNASCTTNCLAPAAKVVHDKLNIKRAFMTTVHSYTNDQKVLDMPHSDLRRARASGLN
                     IIPTTTGAAKAVALVIPDLKGKFDGYSLRVPTPTVSVVDFTAEVEKETTTEELRQMFR
                     DAAKEPRFKGILEAVDEPLVSMDFKGSSYSSSVDLPFTSVLGKEKNNFIKVVTWYDNE
                     WGYSCRTADLAAMMAKTL"
     gene            63705..64877
                     /locus_tag="PLE39_00700"
     CDS             63705..64877
                     /locus_tag="PLE39_00700"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNE66869.1"
                     /translation="MNKKTVKDIDLKNKRVLMRVDFNVPMADGKVTDDKRIKAALPTI
                     KYVLEQGASLLLMSHLGRPKGGPDPEFSLRAASEALSALLEKPVQMAPDCVGADVEAM
                     AKGLKSGDVLMLENTRFHKGEEKNDLDLAKQMAALGDVYVNDAFGSAHRAHSSTEGIA
                     RFLPAVSGFLMEQELEYLGRAVANPEHPYIAILGGAKISDKILVVETLASKCDKLIIG
                     GGMANTFLAAKGLNMQDSLVEAESLETAKSLLGKLGEKLVLPVDAVIADKFDAEANTQ
                     VVDVDKIPAGWRMLDVGPKTISLYQATLNGSKLIVWNGPVGVFEMPKFAEGTFALARM
                     LAESKATTVIGGGDSASAVKKAGVAKQMTHVSTGGGASLEFLEGKELPGVAALLDK"
     gene            65055..66905
                     /locus_tag="PLE39_00705"
     CDS             65055..66905
                     /locus_tag="PLE39_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE66870.1"
                     /translation="MSFNIGENVGPYRILEQLGQGGMATVFKAYHASLDRYVALKVLH
                     PAFNQEQSFISRFQREARVVAKLEHPNIVPIYDYSEHEARPYLVMKYIEGDTLKARLN
                     KGPLSAGEIETVVNAIGAALAYAHRQGILHRDVKPSNVMLATDGLIYLADFGLARIAQ
                     AGESTMSADSIMGTPQYISPEQAMGKKDLDSGTDIYSFGVMLYEMVVGQVPFSADTPF
                     SIIHDHIYTPLPLPRKINPNVPESVQRVLLKALAKDRIDRYETIEELVQAFKDAWTEA
                     GVPMQGTSITMHSTNLRKQVQSTASKPKPAMPNLVNEKPRPQAKAAAKKGSAVWMWLG
                     VALTGFICIIIAFIAIRNNNRIAPLPVQPTLVPTAVILEPSPAIPTQSAPPTFPPLTP
                     PLGTDTTGQPPLNPQGSDTPEIAAAREVVAKNPQDADAHLQLAFALWDAKEIRPSMDS
                     LMQAMGLAGPGNTDFLQKAASGFHQREAWVAATAIYLRLQASYAGAPVPEDIKINTNE
                     SAYKAADRKDFLLLIAFDQIDAIDPRLGHVVRGRQALFNNNLQEAQLQLAEAEKLEPK
                     IPEMFLLKAEIALRNGNTSEARTIFNNTLNNPATPAWMRLMIEELLKTMQ"
     gene            66916..67683
                     /gene="tpiA"
                     /locus_tag="PLE39_00710"
     CDS             66916..67683
                     /gene="tpiA"
                     /locus_tag="PLE39_00710"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNE66871.1"
                     /translation="MRKPLVAGNWKMNKTITETRDLVFTMSMKLREIDGVEKVICPPF
                     MSLMAAAALLEGSGIGLGAQNMHWEEKGAFTGEVSPAMVKELCGYVILGHSERRSYFG
                     ETDQIVNRKLLAAQAMDLVPIVCVGETLEQYESKLTREVVSTQTSQSLRDVSPSYAPR
                     MIVAYEPVWAIGTGKASNGPDANDVVKDVIRPALADLFGNETAQAIRVLYGGSVTAAN
                     AAEFFGQPDIDGALVGGASLKIDEFVAIAKAAASTAA"
     gene            67717..68472
                     /locus_tag="PLE39_00715"
     CDS             67717..68472
                     /locus_tag="PLE39_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66872.1"
                     /translation="MPFTGFLLFILLLLPLVFLQRLLHREIQAVLLILTRNSQLTIGL
                     FSVLFFPGVFLHELSHFLMAKLLQVRTGNISLIPAALPNGRLQMGYVETEETDVVRDS
                     LIGLAPLIAGSLFIAYAGLNRLGFGMLMDLLANGQFALFWTALGYLPQVDDFYLWFYL
                     VFAVSSMMLPSESDRHAWLPLGVWIAGLFALAIFAGAGQWMLENLAPSLNDFLGTVAT
                     LFGLSVAVHVILLLPTMLLHRALSRITGLDIKK"
     gene            complement(68728..70056)
                     /locus_tag="PLE39_00720"
     CDS             complement(68728..70056)
                     /locus_tag="PLE39_00720"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNE66873.1"
                     /translation="MCDTLIASKQATSDNIAIFAKNSDRPPNEGQHIVYFPAQSHAAG
                     SSLKCTYIEIPQVEKTHAVLLSKPFWMWGAEMGVNEHGLVIGNEAVYSKVPANKKPAL
                     LGMDMLRAALERAITPREAVQVIIDLLEKFGQGGNAASHGETMYYHNSFLIADANETW
                     LLETVDKQWAAKQITDVYSISNCFTLGGQFDIASKELVENAVQKGWVKSGSQFDYAEN
                     YSDFIYTNFGKGRVRRATTTSLLETQKGSVTLETMMGILRHHDDEHYDPQNTVAAMDV
                     CMHAGFGPIRISQSTASLVVLLDDKTPLIYVTGTSAPCTSIFKPMWMDTASSLNLGPA
                     PASKYDSASTYWKHEQLHRATLLNYSERIKTYASDRDAMEKKFLSESRRLANATVKER
                     AEFSAHCFAEAAQAEAEWLKRAESIPSQKRALHSLAWNTFNKKAEMPPIR"
     gene            complement(70116..72056)
                     /locus_tag="PLE39_00725"
     CDS             complement(70116..72056)
                     /locus_tag="PLE39_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE66874.1"
                     /translation="MDEKTVPLSRLPVDVKMHHAGRNLLGTDLDYRALFEQTGECVFI
                     IGLDFKIVTANLQALKLLAYEEHQLVGLPVEAIMLIDDSQTRETLLERRTNPSEYTLR
                     KRDGSMLPVELSISMVHNEHNMPTYIQMLARDISEHKQAEANLKRHMLSLSVIGEASA
                     GLFRSSNIETKIPEVLESLGYAVDVFCCALFDIMANSMKVQFQWKSYDASTFDLTSTL
                     EPFLASISENPERVFSVTNVKLQNQEVPTVSILVIPVQGTLGSRGFLALFDKETELSW
                     LTAEFDTVQTTVNLIGAALERLHYEKTIRLSEMRNRILVDALPDLLLRTDLTGHILDY
                     NAHPSHPLYMPRDLVVGKRLDQLWPADVVNMIIETEDQDAFLSSHWVYGFNLPNHQQT
                     FEARLHPISAEEALIIVRDISEQARLDQMKSDFINRASHELRTPLTAAIMMTELLQQG
                     GTEEQMSEYWHTLTSELNRQKNLINELLMAGRLESGRMKLDAVPMELAPILQESVRAV
                     KSIARMRDISIELSVDSDICSVLGDAGGLSQVFINLINNAVKFSPEGSTVDVAAVCGG
                     AEHEARISITDHGLGIPPEAVPHLFERFYRAKNVTVAEIPGTGIGLYIVKSIVDELGG
                     KIDVISELNQGTTFVVHLRLAP"
     gene            complement(72049..73185)
                     /locus_tag="PLE39_00730"
     CDS             complement(72049..73185)
                     /locus_tag="PLE39_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE66875.1"
                     /translation="MENKPIILVVDDEPAIRMGLAATIERHGYGVITAMNGSDAIVKA
                     KKSPPALIVSDVMMPVVNGFDMKKQMSTDPQLASIPFIFLTARTASEDRVAGIRGGAD
                     DYVTKPFVTEELMARIDAVLRRVQTEQARGREQVQHAAQQEMDKLRREVLQNFRHELR
                     TPLSNVIMSLEMAVNTKYKNPDEQAEFIRIALASVDRLDTLVADIILLSDMDQHDLNP
                     TRQLIDVEHHILSPVQKRLHRYPLKRINFVYDIKLANPIKAPRKEFSQALAHLADNAF
                     KFSPEHGQVSLKVRSGENGGGTITIQDESVGVPVELREKVFERFYQISQGDQREFQGL
                     GVGLTVARTVFSNLGGEVKIVDSEKGCCVVATLPDMRPEDVEYG"
     gene            73369..74631
                     /gene="obgE"
                     /locus_tag="PLE39_00735"
     CDS             73369..74631
                     /gene="obgE"
                     /locus_tag="PLE39_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNE66876.1"
                     /translation="MFIDHVTIHVKSGKGGDGMVHFRREKFVPLGGPDGGDGGRGGNV
                     IFEVKATLNTLSAFRPNEKFAAEPGKNGGGSQMTGRGGKDLIIYVPPGTVLYDAETGA
                     LLGDLTTPGQQLLVCKGGRGGLGNQHYATSRNQAPRTAERGEPHEEKMIRLELKLIAD
                     IGIIGLPNAGKSTLLAALTNAKPKIGDYPFTTLEPNLGVAKIDDDTTVVLADIPGLIE
                     GAAEGAGLGHDFLRHIQRTRVLIHMVDGQSEDPLADYTQINNELALFDPNLAKKPQIV
                     ALNKTDQPDVQERLKSIKASFKKKKIELFTLSAMARTNTRDLLIAAHQRLADMPVEEV
                     EETLPVYKPDVDPNHFEITREDDGSWRITGVAIERSAKMTYFEHDGSLRRFQKLLQRL
                     GVDDALREAGVTDGDTVFIGNFELEWKE"
     gene            74647..75054
                     /locus_tag="PLE39_00740"
     CDS             74647..75054
                     /locus_tag="PLE39_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66877.1"
                     /translation="MAQLSPNKQRALTIGMIVLGVIVAVFFGMRAFRAFNRFNGHRPP
                     PQGKVETDVELIRGWMTVPFISQMYRVPDRIIFEELNIPEKGNMEKSLKDLNQEYYPN
                     EDGFVIEMVKQIVQANLPNEPHDPPEPPTPPNP"
     gene            75064..75672
                     /locus_tag="PLE39_00745"
     CDS             75064..75672
                     /locus_tag="PLE39_00745"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNE66878.1"
                     /translation="MSDFFLTQVINFGTPLVGLILFLGALGFPVGASVVVIAAGAFGQ
                     QGILDIPSAAIYGLLGAVIGDTLSFGMGYYAKDAVQRRFGNSAAWQGATETFQKNAGM
                     AVFLTRWLITAIAIPTNLIAGGSGYKYSRFISYDLTGEIVWIILYGGLGYLFGSQWEL
                     VYDFMSNFGGLILGIVLMGFGIRQALQWQKNSRDKINQKAEA"
     gene            complement(75697..76188)
                     /locus_tag="PLE39_00750"
     CDS             complement(75697..76188)
                     /locus_tag="PLE39_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE66879.1"
                     /translation="MSKDTVQDGLVVSMDYKLTVDGEVLDSSDEAGPLQFLVGYGNII
                     PGLEKEMMGMKIGDSKDVVVQPEDGYGEFDDDAFMDVPRTEFPADMKLEEGMELHVTD
                     EDGNHQAAYVASFDKDTVRLDFNHPLAGAELSFYVKVVALREPTEEELDHGHVHEDGH
                     HHH"
     gene            76327..77208
                     /locus_tag="PLE39_00755"
     CDS             76327..77208
                     /locus_tag="PLE39_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66880.1"
                     /translation="MRYDLCLPWYWEYDDAFVGMVERTCIEQGLSLWQIKPDNLLESI
                     TALYKGVTTFKTLLHRGQGEAIFDPIPRWAKEFNARRINPAEVSAWSEDKATMHLELI
                     SAGLHTPHTILLPPFLEQPVVSEINLAPLGGNFVIKPSNGGGGEGVIIGANTIEEILH
                     ARMEYPEQKYLLQAHITPRVIEDTPAWFRVFYANQETYPCWWNTETHAYRPVSAEEES
                     QHRLGRLRDVTKRIASICKLDWFSTEIALAEEFIVVDYVNDEIDTRVQSQALDGVPDE
                     IMECVAKQLVKIAKENA"
     gene            77205..78284
                     /locus_tag="PLE39_00760"
     CDS             77205..78284
                     /locus_tag="PLE39_00760"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNE66881.1"
                     /translation="MKNVRARDLGIPFEGLTGENNAITDVSNVTVGYATVIEGPSART
                     GVTIIHPRGDNDHTPVFAGIHSFNGNGEMTGAAWVEEGGMLEGPIGITNTHSVGIVRD
                     SIVAWQVKNDALYQKWSCPVVAETADGWLNDMNGFHVNENHVLQALNSAKNGSIEEGN
                     VGGGTGMLCYEFKGGTGTSSRKLPAKLGGWTVGVLVQANFGRRYQLTIAGTPMGQYFK
                     DDAPFTNGENPFRQDDGSLIVIVATDAPVLPHQLKRLAKRATIGMARTGSLGGNGSGD
                     IFLAFSTANPQAAHDGEMGVANIQSLSNNYIDPILAATAYATEEAIINAMVAAKDMKG
                     HNDVDAKALPHDGVREVLKRYNRIG"
     gene            complement(78399..78923)
                     /locus_tag="PLE39_00765"
     CDS             complement(78399..78923)
                     /locus_tag="PLE39_00765"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNE66882.1"
                     /translation="MPKNIVLTLTGRDRIGLVDHVTSLVVKRGGNVGASRMAHLGGEF
                     AMLMLIALPDNEAGNIDQDFQSLRGEGYQVSIVQTEDSKKYEGWLPYEIEVTGADHEG
                     IIHDIAHYLASRGINIENMDTSSTPAPMSGTPLFTMKGVVLAPPKLNFHAWSDELEEI
                     GDKLNVNVIVGLVK"
     assembly_gap    79010..79109
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            79155..79715
                     /locus_tag="PLE39_00770"
     CDS             79155..79715
                     /locus_tag="PLE39_00770"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HNE66883.1"
                     /translation="MQTLLKLSQRIDAITEYFGKASAFCVLLTIVIGFYNVVARYLGR
                     FIGFKLTTNALIELQWYLFSLTFLLGFSYILKHGENVRVDFLYTNWSPKTKAWVDLVG
                     HALFLVVFCIIGLYVTTHPVLQSWGRLPDGTFGTWELSPDPDGLPRAPIKSMILVGFG
                     MLLFQTISEIIKNIAIIQRPQTTDSK"
     gene            79762..81108
                     /locus_tag="PLE39_00775"
     CDS             79762..81108
                     /locus_tag="PLE39_00775"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNE66884.1"
                     /translation="MTHFEWLAIVMFVGFFIFIMSGYPVGYSFAATAILFGGIGYALG
                     AFDLNLLKLLPSRWFGTMSDFTLLAIPFFIFMGAVFERSGLAEELLETVGRLMGRVRG
                     GLALAVILVGTLLAATTGVVAATIIMMGMLALPTMLRHGYDKQLATGVITASGTLAQM
                     IPPSLVLVVLSDQIGVPVGDLFLGAVIPGLLLSSLYALYVIIITRIKPDLAPALPPEA
                     FSVTGADLLRKSVRVIIPPGLLVFSVLGSIFFGLASPTEAGSVGAVGACLLALMNKRL
                     TTKLIQDAAMSTARLTALVITILFSSTFFSLVFDGLGGQRLITDLLVNLPGGFWGFII
                     VANIAIFLLGVFLEFVEICFIAMPLFVPAAHALGIDMAWLAIVFAINLQTAFISPPVG
                     FSLFYLQTTAPKEVPTSDIHKGALPFMALQVLALIIVIIFPQTVTWLIELSAKSLP"
     gene            complement(81177..82280)
                     /gene="dctP"
                     /locus_tag="PLE39_00780"
     CDS             complement(81177..82280)
                     /gene="dctP"
                     /locus_tag="PLE39_00780"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HNE66885.1"
                     /translation="MNRRDFLKVAGMGVAASALAACAEPAPAAGNAAAEDLPVLDWQM
                     ATSWSTGLDILFGATQKFAERVKALTGGKFTITPRAGGELAKATEVLDVVSSGAVPIG
                     HTASYYYIGKSWVLAFGTSLPFGLTASQQNAWLYNGGGLELLQDFYAKKFNVIQFPAG
                     NTSAQMGGWFRKEINTLADLQGLKMRIPGLGGQVMSALGVQVQNIPGGEIFQALETGA
                     IDAAEWVGPYDDEKLGLHKATGFYYFPGWWEPGPSLELQINLDEWNKLPALYQEAIKA
                     ASYEANVAMAAHYDAVNGEALERLLAGGVKLMSYSDEIMQAAQEKAFELYDAKSAEDA
                     EFKAVYEKWMEFRTRIQKWSFTNEFALLKFMAG"
BASE COUNT        20407 a        22734 c        19465 g        19520 t
ORIGIN      
        1 ttgcatacgc aaggccttta tcgtaaaatc ccattaacca aacccaaagg agaatctatg
       61 gcaacaaaga caatcgatga aacccgcccg atgacagaag aagcagactt tctgcagatc
      121 aagtccattg atcatgttca tttttatgtc ggtaatgcca agcatgccat gtattactgg
      181 tggaaggcct tcggcttcaa acccgtggcc tactccggtc ttgaaacggg caaccgcgaa
      241 ttcgcttcgt acgtgctcga atcaggacag gcgcgcatcg tcgtttcggc cccctacggc
      301 ccatccagcc cgcttgcatc acatcacatg gtgcatggtg acggggtcaa agtcattgcc
      361 atggaagtag acgacgtgga aaaagcctgg caggccacca cctcccgcgg cgcaaaatcg
      421 gcctgggccc cccgcgaaga aaaagatgac cacggcatct accgcacatc ttcaatttat
      481 acctacggcg aaaccctgca tacattcgta gaccgagccg actacaaagg cgtatttgcc
      541 cccacctacc gccccatcaa agacaaggaa gcccactcca cggggctggc cgccattgac
      601 cacatcgttg gcaacgtgca gcttggcaaa atgaatcagt gggtcaattt ttatcaccat
      661 gtcatgggct ttcgccagct catgcatttc gacgacaaag acatttccac tgaatattct
      721 gcactgatgt cgaaggtcat gcaaaatgga aatgggcggg tcaaattccc gatcaacgaa
      781 cccgctgaag gcaaacgcaa gagtcagatc gaagaatacc tcgattatta cctcaccccc
      841 ggcgcacagc acctcgccat catcacaggc gatattctca ccaccgtaga ccaactccgc
      901 aaaaacggcg tggaattttt acgcgtaccc gatacctact acgaagccct cccagagcgt
      961 gtcggctcga tcaaggaaga ttacaaaacc attcaggagc tcggcattct ggtcgacaag
     1021 gacgatgaag gctaccttct gcagatcttc acccgcccca ttcaagaccg ccccaccatg
     1081 ttcatcgaga tcatccaaag acacggcgca cagggattcg gcaaagggaa cttcaaagcc
     1141 ctgttcgaat cacttgaact ggaacaacaa cgccgcggca atttgtagcg acctgtgacc
     1201 agtcgtcagg cattgacact gatgactggt cacttttgtt tactgaccaa ttcacccaca
     1261 tgattttcgc cacctgccgc acgcctcaca ccgcgccgag gcctgccctc gtctcgaatg
     1321 acagaatcta cccgctgccc ctcgcagata tgcatgccgt catcgcactc ggagcggata
     1381 aagctgccag cctcgcttca caggaatcat ttgcacctgc cgaagttcaa ttccttgcac
     1441 ccattcaacc cgccagtctg cgcgatgcct acgccttcga acaacatgtg agaaccgcca
     1501 gccaaaaccg cggacgcgat gtaccgcagg agtggtacaa attccctgct ttttattaca
     1561 ccaacccgcg aaccatcttt ggccccgacg aagtgattcc caacccgcac tacacttcgg
     1621 caatggattt cgaactggag atcgccgtca tcatcggcaa aagcggacga gacatcaaac
     1681 ctgaagatgc ccctgcacac atttttgggt acaccatctt caacgattgg tccgcccgcg
     1741 atgtgcagcg cgaagaactg cggatcgggc ttggcccagc caaggggaaa gactttgcct
     1801 cgtctttagg accagtgatc gtgacgagcg aagcgcttgc cgatcgggca gttaatcgcc
     1861 cgggcgtgta tgatcttatg atgacggcaa aaatcaacgg cggtgaaatg tcccgcgcaa
     1921 atttaaagga tattttctgg tctttcggcg agatcattgc aagggcctca gacacatgtg
     1981 aattacaggc aggcgatgtg atcggctcag ggacggttgg cacgggttgt cttttggaat
     2041 tgacgaaagg ccaggggccg tggctgaacg caggcgatgt ggttgaattg gaaattgaaa
     2101 aaatcggaac gttgaagaac accattggaa caaaaccaac cccgttgttt tgaaaggaga
     2161 tttacaaatt ggaacagaac aagatcatca ccgtccgccc aaaagagacc ttcatctcaa
     2221 agcagcaatt accgaacttt gaaggcatat caacccaaac ggcaggcact aaacatttgt
     2281 gcatgcacct tgtcatcatc ccgcctggcg gaagggccgt ggcgcattat cacaatggct
     2341 acgagaccac catctacatc atcaaaggtc acgccgaaac caggtacggc gaaaagctcg
     2401 aacattccag catcaacgag gcaggcgatt ttcttttcat tccgcccaac ctgccgcatc
     2461 aacccatcaa cctgagcgaa accgaggaag ttgtggctgt ggtggcgcgc aacgacccca
     2521 acgaacagga aagtgtcgtg gtttatcaac cgcaggattc ataaacaaaa gaaacggaga
     2581 agcacatgcg caatacgcac ccgcgtgatg aagataccta caccagcgat gtgtttgagg
     2641 tgttgttcaa ttatgaagtc acccgctcaa ggcggtatcc atctccgctg gccctgcttt
     2701 atattgagat ggcacccatc acactcgacg cagaattaca ggctcaggct cccgcggttt
     2761 tcgccgctgc gctaaattcg catctgcgtt cggtggatat cccatccacc tatggcaggg
     2821 cctacagggt tttgttgccc agttccaacg aacatggcgc acgtgccgtt tgcgaaagat
     2881 tgttgtcggt ctatcgaaac aaattcgagg tcgccaatgg ttccattgca ttctcactgc
     2941 acatcggtgc caccgcccac aatggtgggg ccgagctctc cgctgcggaa ctcatccaaa
     3001 aagccgaaga agccttgaaa caggcaaaac tcaaaggtcc caacacctac atggtgattt
     3061 agattttttc gtcaccactc aaggagatat tcatgccttt ttatcacaag cttggcaaga
     3121 tcccgcataa gcgtcacacc caattcaaaa aggccgatgg aaagctatat cgtgaagaag
     3181 tgatggggtt ggagggattt tcctccattc aatccattct ctatcaccac tttcttccac
     3241 cacgcgtgaa aatgacggag gaccttggca gcgccaaacc tgaatatgtg gactttggcc
     3301 cgatccgtca ccgcgccttt agaacggatc aaaccccgct cgggcctgat cctgtttcgg
     3361 cgaggattcc actcctcggc aataacgacg tcatccttgg cgtttcacgc gcaaaagaca
     3421 gcatggggta tttttatcgc aactcacaag cctatgagac ctggtgggta catgacggca
     3481 gcggcacgct taaaagccaa tttggaaatc tgccgtttcg caaaggcgat tacattgtca
     3541 ttccatttgg ggttacctgg caaatgcaac taaatgagga atgtaaattc ttcaccatcg
     3601 aaaatccaag ccagatcgag ccgccaaaac gctaccgaaa caactacggt caactgctcg
     3661 aacatgcccc ctactgtgaa cgagatatcc gcctgcctga agaattggag acccacaccg
     3721 aacgcggcga ttttgaagtc cgcgtgaaag tgcgcgacag attatcacgt cacatccttg
     3781 attttcaccc tttcgatgtc gttggctggg atggttatct ctacccatgg atcttcaaca
     3841 ttgaagattt cgaacccatc acagggcggc tgcacatgcc gccacccatc caccaaacct
     3901 ttgacgctca taattttgtt gtgtgttcat tcgtcccccg tctctttgat tatcaccccg
     3961 agggcatccc cgctccatac aatcattcca acatccaatc ggatgaggtg ttgtattatg
     4021 ccgagggaaa tttcatgagc cgcaagggaa ttgaccgcag cgatatcagc ttgcatccgt
     4081 ttgggcttcc gcatgggccg cagccaggca tgacagaagc cagcatcggc aaagtggaaa
     4141 cgaaggaact cgcagtgatg gtggatacct tccacccgct gaatctcaca aaacaggctg
     4201 ctgccatgga aaagcccgaa tacatgtcca cctggctgga aggcgatgga gaataatgtt
     4261 gaatctgagc acagcccaca tcaaggataa aaactttcgc aagctctcgc aaaaagttgt
     4321 agcggagatg aagcggctat ccatacctgg tgttgccatt ggagtttggc acaaaggcag
     4381 ggaattctct gacgggtttg gaatcaccag tattgagaat ccgcttccag ttacgtcaga
     4441 tacgttgttt caagtcggct ccatcagcaa aaccttcacg ggcaccctga tcatgcaact
     4501 ggcggaacag ggcaaagtca acctggatgc ccctgtgcat acttacctca aggacttgaa
     4561 actgagcgac aaggcggtgg aaaagcaggt caccgtacgt catttgctta cccacacagg
     4621 cggctgggtc ggagattatt tcaacgactt cggcaacggt gacgatgcgt tggctcgcat
     4681 ggtaaaggac attgcgcacc ttccgcaggt gatgccgctc ggagaaatct ggtcgtacaa
     4741 caatacaggc ttcaacattg cctcacgcct gatcgaagtg ttgacgggga agccttacga
     4801 acaggccgca caggaaatgc tgcttgaccc gctgggtttg aagatgagtt tctttttccc
     4861 aagcgacctg ctcatcacgc atcgttttgt gacgggacat tacaacgaaa ataaaaaaac
     4921 ctttgtctca cgcccgtggg ccattgggcg ggcgggcaac ggtgtgggtg gcgtggtcag
     4981 cacggtaaaa gatttgctgg cctatgcgcg ctttcacatg gggaatggca gccgcatcat
     5041 caagcgcaaa tctttggaag ccatgcgcat gccgcagatc agtgctggtg gacgcggcga
     5101 gatgggaatc acatggttta tcaatcgttc aggttcactc acccgataca gccacggcgg
     5161 cgccaccaat ggacagcaag ccctgttcgt atttattccc gaaaaggact ttgcgctggc
     5221 tttgctcacc aacaacgata atggaggagt gctgaacaac aacctgttgg gacttgcgct
     5281 tgaggtgtat tttggcatca agccgaaact tccgaaaccg atcaaggtca gcacaaacga
     5341 attgaaggaa tatgtcggct cgtatcgaat tggcacagaa gcgttcgacc tgaaactcaa
     5401 gaatggcgaa cttatctttc agcacattcc gctgggcgga tttcccacgc ccgatagccc
     5461 gcccggacct gccatgccac ccatgcgggt tgccttctac gaaaaagatc aaaccctgat
     5521 gctggatgag ccaatgaaaa acggcatcgg cgatttcatt cgcgacaaaa atggacgcgt
     5581 gcaattcttc cgcatcggcg gacgcgccca taaaaaaata aagtaatgag gagcattatg
     5641 aaaaaattca tggggttgtg tcttgtggcg gggattttca tttcggcttg ttcgatgcca
     5701 tctgccgcag gaccttcgga ctcagaaatt tccactgccg cagcgttgac cgttcaggcc
     5761 gtcatcaact ccacaccgct tgccagccca accgcggcag gtggtcagca gttagaccca
     5821 gaggcggcaa caccggttgt gatcacaccc acgttctccc agccaatggc cagtgtggga
     5881 gatgtgatca attgccgcag cggcccaggt acaaactttg aacgcgtcac ccagatcctg
     5941 cctactgatt cagtaaagat cgttggtttt tatccgccaa attactgggt cgtcaacacc
     6001 aaggatggtg attgctggat ctcgggggaa ttcaccacgc ctgttggaag ttttgccgca
     6061 gttcccacgg tcaccgcacc gcccacgcct gaaggcggcg gccctgaagc agccaccttc
     6121 cccaagaacg gatggacgtt cttttgctat ggtccgggca aggccgatat cacgctctcc
     6181 tggaacgaca acgccgaaaa tgaatcggga taccgaatca tcagagacgg agaaaaggtg
     6241 gccgaactgg ccccgaacac aaaatacttt gcagaaacga tcgatctcaa ttctggtgaa
     6301 agtgtggctt atcaaattca ggcctacaac gaagcaggca gttccgatag cgcaacggca
     6361 aatatcacct gtccctaaat ctcccatcat aaaaaaaata tgccgacact ttcgtgtcgg
     6421 catatttttt attcttcacc aaattgctct ttcgcctttt tcgtctgctt accgcgctca
     6481 tctgtggaga ggatacgctt gcgaatacga taattttcag gggtcacttc cagcaattca
     6541 tcgtcggcga gatattcgat cgcttcatcg agagacatct gtcggggtgg ggtgaggcgg
     6601 atttccatat caccacccga agcacgcata tttgtcaaat gcttcttctt gcagacgttt
     6661 acagaaaggt caccaccacg ggcgttttca cccacaacca taccttcata cacttcaaca
     6721 ccagggccaa cgaacaactg tccgcgctct tcagcagctt tcaatgcata cgccgtggtt
     6781 gagcccgctt cccaagccac aatggagcct tttgttcttg aagagatggg gccagccatt
     6841 tcatcgtagc cgctgaaaat ggtgtgcatg acaccttctc cgcgggtgga agtgaggaat
     6901 tgataacgga agcccagcaa accacgggtg gggacaacgt acacgatacg ggtcataccc
     6961 tgacctgcgt catgcatctc catcattttg cctcttcggc tgccaagcat ttccacaacc
     7021 acacccacag tttcgttgct ggtctctacg tgtacttctt catacggctc aagcaaaacg
     7081 ccgtcatcag ctttgtgata gatcacttca gggcgagaga cctggaattc gtatccttcg
     7141 cggcgcatgg tttcgatgag gatgccaaga tgcaactctc cacgaccaga aacgaggaaa
     7201 ttctcggccg attcagtatc ttcaacacgc aaggaaacat tggtacgcag ttcgtcatac
     7261 aaacgctcac gcaatttacg cgaagtactc caggtgcctt ccctgcctgt gaagggcgag
     7321 gtgttcacac caaaagtcat gcgaaccgtc ggctcttcca ctttgatggt tggcagagca
     7381 acggggttgg cagggtcggc aagggtttca ccaattgcga tcccttcgag gcctgccagc
     7441 gaaatgatat cgcccgcctt tacttcatca atttcgatct tgttcaaacc tttgaatgag
     7501 taaaggtaac gcgcgctttc aggaaggatg gagccatcca atttcatgcg ggccagtttt
     7561 tggccagctt tgatcgtacc tgcaaagaca cggccgatgg cagtgacgcc gcgatagtcg
     7621 tcatatccca gcatggtgac gagcaactgt aaaggagcat ccacatccac cttgggtgcg
     7681 gggatatgct tgagaatcac atcgaacagg ggtttcagat caggcccggg ttcaggggta
     7741 agacccgctc ttcctgcggt ggcctgggca taaacaacgg gaaaatccgc ctgttcctca
     7801 ctggcgccaa gttcaatgaa caagtcaaag gtttcattga gcacacgggc gggttccgca
     7861 tctttacggt ctactttatt gatgaccaca acggccttgt ggcccaactc aagcgccttc
     7921 ttcaatacaa agcgggtttg aggcatgggg ccttctgccg catctacaag caaaagcaca
     7981 ccatcgacca tgttcattac gcgttcaact tcaccgccaa aatcggcatg tccgggcgta
     8041 tctacaatat taactttgac tgtcttgcca ttttcatcga tcaatgaaat agctgtgttc
     8101 ttggcaagaa tcgtaatgcc tcgttcacgc tcaagatcgt tcgaatccat tacgcgttct
     8161 tcaacatgct ggttctcgcg gaatgtatgt gattgtccga gcaatccatc cacaagggtg
     8221 gttttgccgt ggtcaacgtg ggcaatgatg gcgatgttac ggatgtcagt tcgttcgata
     8281 agcatagggt ctctttaatc ttttgggcgt tgttttatga aaggccgtaa gacacacaaa
     8341 aacccccgac ggggaggccg agggcttgaa atcagtggtt cggcgtgctg gtgtgtatat
     8401 tatcagattg tgatgttttt gggtagggcg aggcagattc ctgtcatttc tgggcagata
     8461 cccatccaga acaaagcgtt taatattcta attaacttga aacgcacgga actggcattt
     8521 acccagtaaa attggcctac ttatgggaaa tccatactac atcacgcata tcatcatcct
     8581 gctgattagc agtacgatcg ggattctttt gctgcttcgg gtttggagcc tccgctccct
     8641 gcctggcgct catggcctga tcttaaccat tttatgcgtg gtggaatggt ctttagcgta
     8701 cgcgatggaa attgtgcgcc tagacctgac cgaaaaggtc ctttggggca agctgcaata
     8761 ttttggaatt tcctacgccg cactcggtat gttcattttt gcagtcaatt acagcgggca
     8821 tggggcacgg cttacttcaa cccgaaaagc ttcgttggcg ctacttgcaa gccttgggtt
     8881 tattgccgcc ctcaccaatg accgtcatca cttgatctgg aatcaaatcc agttcaccaa
     8941 caatattccc tttggccctc tggaattaag ccatggggta ttgttctttg ttgtggtcgc
     9001 ctttcaatat gctttgctgc tggctgtcac agggatattt ttacagatcg caatacatgg
     9061 gcaaagcctt tatcgagcac agtctaaaat aatgcttgtt ggcatgtcat ttccgtggct
     9121 ggcaaacctt atttatgtaa ccgggtggaa tccatacaaa ggggtggact tcactcccat
     9181 cgcccttacc ttctcagcca ttgcattttc catcagtttc ctccgctatc ggttcatgga
     9241 ccttctaccc gttgcacacg attctgtgtt taatgccatg aaagacgggg tcattgtact
     9301 ggactccaaa gaccgtattg cagatattaa tccgatggga agtttcattt tccaaaatcg
     9361 tggaaactta ttggggaacg aaatcagggt gctattccca gattggaaca actggataag
     9421 cccgggacac cacgatgaaa tccaccagga actttgcctg gactataacg gtgaaaccgc
     9481 aacctttaag ttgcgaatca caccgatcct cgaacaacaa ggtgagcata acgggaatat
     9541 tattatcctc agcaatatta ccgaacaaaa aaggttccag gaacagatca tggaggccag
     9601 ccgccttaag agtcagttac tcgccaacct tggccacgac ctgcgctcac cgcttggcgc
     9661 aatcatcggc tatgctgaaa tgttagaaga tggttctttt ggcccgatga gcaacgaaca
     9721 agtgaaagcc accgctgaaa tcctcgatgg cgcaaatcaa ctgctgtctt ttatcaacaa
     9781 tcttgtcggt caggctcaaa ttgaaacagg caaagtcatc ttacgggaat atcctttcaa
     9841 cgtggaagaa gtcattggac cgcttctttc cacattgaat ttccatgctc acaaaaaagg
     9901 acttatcctc attcaattcg tcgaccctga tcttcccaaa aaacttgtcg gtgaccaatt
     9961 ctggttgaga cagatcgtca tgaacctggt acacaatgca gtcaaattta cagatcaagg
    10021 ttctgtgacc gtccgcttta tcgtacaagg tgaagatcag tgggccatac aggtatcgga
    10081 tacaggcatc ggaatccccc cggaggcgca aaagcgtgtc tttcaagcct tcgaacaaat
    10141 caacagcatg gaaaacgcaa aacaaagcgg gtttggattg ggactttcca ttgttgcaaa
    10201 attaacatcc ctcatgaatg gaaagatcga attgaagagt gaagcaggca ctggaagcac
    10261 attcactgtc atccttccgc ttaaagaagc cgtggaacag gaaggcccga caatgttcgt
    10321 ccgttagttc tacagccttc ccgccttgcc agcttttttc aggtcacgat aattccacca
    10381 cacctgccag atacgaattg cggcttccaa ttggatctta attgacattt tcgattttcc
    10441 ccagcgtcgg tcggcaaaat aaatgggggc ttcgccgatc ttaaactcaa gacaatgtgc
    10501 aagataggcc atctccacca gaaatacata tccactggaa tgaatccgtt caaacgggac
    10561 ttgttgcagg gtctcacgcc gccacatacg atacccggtt gtgacatcat gcaaaggcaa
    10621 ccccagaatc gtgcgtgcgt aataatttcc aaaagccgac aggctccgcc gccataacgg
    10681 ccagcgctca tccactgaac caccctttac ataacgagac ccaagaacaa tatcattgtc
    10741 tgttaagagg tttgccatat ccaaaagcgc cgacgggtca tgcgaaaagt ccgcatccat
    10801 ttgaacgatg gcgtctacgc tactgtccat tactttttgg aagccattta aataggcgga
    10861 acgcaacccc atcttacccg ggcgatgcag cacatcaaca cggccggggt atttcacaac
    10921 caattcatca gcgatacgac ctgttccatc agggctgtta tcatccacga ccaacaagtt
    10981 gatatcaagt ggtagggaaa ataaagcgga gaccagtttg ggcaggttct ccgcttcgtt
    11041 atacgtaggt gttacaactg taatacgcaa acaaactcct atttctttaa ccgaacaagc
    11101 tccgctttga tttgacccac atctgagaat ctatccttta atgaagcgat ctcagccaat
    11161 ttgaccccaa gcctgttcct gctattcacc atttgttcac ggtctttgat cgggtagaaa
    11221 tattccaccg actgttcaag gcgggctcgc agcttttcac ttaaggctgc caaatttgaa
    11281 ggcggaataa ccaaaataaa atcagttgta ctaagatggc ccaggaaatc ctcggggcgg
    11341 ctcgtttccc gcattgtgtt tacgatcatc aagctgattg cacgcaacac atcatcagag
    11401 gcaacaaacc catatgcttc acggaaagaa tccatgttat caattgaaac aaagaggacg
    11461 gcaaccccgt cttttccaat cacttccgag agtttttcat ccaccaatgc accttcaggc
    11521 aggccagtca ctgggttggt caaagaaccc tggctgacac gcttaagcgc attgcgtacg
    11581 cgcaagcgta attcctgtac atcgaaaggc ttggtaatgt aatcatccgc accgatttcc
    11641 aacccttgca ggcggtcaga gcggtctcgt ttttctgtga ggaaaataat ggggacttca
    11701 gaggtgcggc ggtcattccg aaggcgacgg gctacttcat aaccgtcaat gtccgggaga
    11761 cgaatatcta gaataacaag atctgggtga atggtttgac aggagcgaac cccatcctca
    11821 ccccagttaa cagtaaaaac atcatagcct tgtacgcgaa aataggcgtt cagcatttca
    11881 gccacgtcaa ggtcgtcttc aatgatgaga attttggact taatgtctgc cacgccaaac
    11941 ctgcacttga gttatgaaat cggctctttt tggacaatga attcacaaac agagtcacca
    12001 atggcaacac atttggattc attgacgcgg aattcatttc cgccagaaac ccatttgagg
    12061 ctttcctgca acaaacctgc tgcaatgaaa cagacgggtt tatctgctcc ggtacggccc
    12121 caacaacaag gacacttatg aatggtccat atccactcag tatccttttc ttccactgtt
    12181 gtttgctggt cactcaattg gctgaaaatc tttgcaaagg cgggaagacc aattctcagt
    12241 ttggattgaa gcggaagtac tacaaaggcc aaatctgcag caccagccaa tgcaccaaaa
    12301 ttccgcaagg catctgagaa tgtggcacgc cctgcacgaa gggctaaacc gcgtccgccg
    12361 cgtggaccgt acatttcttc caaggcggag ccgattgcag aaagttcagc aaagtcaaac
    12421 cccttgtcca gattatccgg agggtagttt tcaatataat tattcaaacc tgccaaattc
    12481 aaaatggcat ttaagccatt tttacccatc acctcttcca aagatttgat ggtgatcata
    12541 ccgaacttat tgggataaaa taaaccgctt ttttgcaggg gggccataac tactccacta
    12601 gatcaacttc gccagatctt cggcggcgcg tcgcatatca aggaagatca ggccaagctt
    12661 ggcctgttcg cgtgccagtg cagtgagtac agcttcatca ccgacagaca tcaaaacaac
    12721 ataccccttc aagcctttga tgtaaacctg ctcgagggag ccacgtccta attcacttgc
    12781 aatacgctca cccaatgaaa gcatggccgc agacatggct gaaacacgat cttcttctac
    12841 gccttgcgga agggcagatg caatgctcaa accatcaaca cttacaactg ccgatgcttc
    12901 aatatcgggt gacgaagcct gcaactcacg caggcgatca accatttgtt gtgaacgatt
    12961 tttggacaac cagccctcct gtggggatgc gaacatccat tttgaaagga tgcaagcttc
    13021 tttataatag ttgtactatt ttagaacatg ggatgcattg tgtcaagttc ggcaacttct
    13081 attcttacaa aacagcaaac tacacatgct ataatttccc gccatgaaaa gaatcactac
    13141 aatccttttt atagcatctc ttttcctcgc aacaacacaa tctgttcatg cgcacaaaca
    13201 aacgcccccc tcagaacccg aaagtggtgg ggttgtttgt gaacccgccg cctatctcgt
    13261 tcccccagat gactgcctcc ccattggacc ttcaacctac ttaaccaacc ttgcccacct
    13321 tggaattacc ttccctccca gaccactccc cgcttcaaaa cctgacctga gcctgactca
    13381 gttaccttat ttttatttcc acctcgacaa agactatgta ccagtttatg gagccccagg
    13441 cgaaaaaggt cccggcgggc aacaatttcc acctggtttt gtatatgtct cctacattga
    13501 ccgtgtggaa caaaatggcg tttattacct catgcccaac ggcggctgga ttcccgggaa
    13561 aggctcacgc gttggcgaaa tatcgaattt tcaaggcttg atcttttctt ccacacctaa
    13621 aaactctttt ggctgggcat ttgaaatgtt acctgtcatg aaatcgcctg gctacaatgc
    13681 agcccaaaca ggcaaacaaa tccaaccctt tgaagttgtg atagtttatg atgtccaaac
    13741 catggataat gaggaatggt acatgatcgg accagatcaa tggctggaag gtcggaaggt
    13801 ggcccgcgtc atcgtcaaca ccacaattcc gcagggcgtc acatcaaatc gctggatcga
    13861 tgttgatctt gctgaacaaa ctctggcagt atacgaaaac aaccgactca tctttgccac
    13921 cgtcatcgcc agcggacttg acccattctg gacacgcccc ggtctttttc aaatatttga
    13981 aaaaaaagaa acagaaacca tgcgaaacaa tgacatggca gatttctatt acctcgacaa
    14041 cgttccatgg accatgtact ttgatggagc gcgagcactt catggcgctt actggcgcac
    14101 ccgttttgga tacccacagt cacatgggtg tgtcaattta tccgttggcg attcgcattg
    14161 gttattcaac tgggcagttg ttggggattg ggtctacatt catgaccgta ccggacagac
    14221 ccccacagat cctgcacttt acactggcgg agcgtattga catcaaaata ccatcatggt
    14281 aaactatcgc ccgcttagct aattggtccc gtggtgtagc ggcctaacat gcggccctgt
    14341 caaggccgag atcgcgggtt cgaatcccgt cgggaccgcc ttattgaagg caaatgctgt
    14401 attcacagca acaaaaagac cacattttca tgtggtcttt ttgttatcca tttattgctt
    14461 atgtcgtact gcttcgttct gcaagcaact cttgcaaacg agggtggttt tcataatacc
    14521 cacgataact ttcgggcaac tctaccgcaa tacggcacat gatttcatct gttgcttcgc
    14581 gcatggcctg ttcgcggccg ctgcctttgg ggatttcaat gaaaaacggg tcgccagcaa
    14641 ctgcttttat ttttgaacgg cgaaaccttt tcaaatttgc aaccacgccg ccatcttctg
    14701 tacctgtcaa agctacaggt atgacgggat atccgctttt actggcaagg aaggccacac
    14761 ccaatttacc ttgctgcatg gatgcagtct ttgaccgtgt gccttctggg gcaatcacca
    14821 tcaagccacc cttttgcata cgggccaaaa tctcgcgcaa ggctgcaaaa tcagcttcaa
    14881 aacgattaag ccagatggca ttcgctgcac gtccaatcgc cccaaaaatt ggatgatctt
    14941 tatatttctc agccaccgcc atgatgatat cctcacgatc aaggatatac aacaagacag
    15001 ccgtatccag gcggcccaaa tgattcgcgg caatcataac attcccctgt ggcagttttt
    15061 caatcccgac cacttccaca tcggcaatga tgttcataac aaatcgaaca acggaacgaa
    15121 gagttctaaa tttcatgggt ttatttaccg gacaacaatt ctttcaattt gggatgctcg
    15181 gcataatagc cgcggttctt ttcaggcaac attaccgcaa tacgacacat gatctcgtca
    15241 acatattcct gtaaggtttc atcacggttt tctttcggaa aagacggcag ggtgaatggc
    15301 ttgcctgcgg tcaggtgaat ctctgttcgg cgaaaccgct tcaggttgga taaaacaatt
    15361 cgatcttccg ttccagcaat ggcaactgga acaatctgcc atcctttttt agaagcaaga
    15421 taagcgaccc cgggcctgcc tcgtgccatc ttttcatcgc gggcacgggt cccctcgggg
    15481 gcaatgacca gggtctgacc atcttccatt cgtctccaaa tttcacgcat cgccttcaag
    15541 tcaggattga agcgatcaat gaaaataaag ttcaaatgtc tgcctaaaaa tctccaaagt
    15601 ggattttcct cccatttttc agcaacaggg atgaataaat tccagttatc caaagcataa
    15661 tacgccatgg gagcatctaa aaagccgaga tgatttacgg cgatcacaaa accgcccttt
    15721 tcggggagat tttcgtaccc accgatcttg atggtcgtaa aggaattcaa aacaaaaggg
    15781 atcaaccagc gcagaaaact tctcatttgt taattttaca cgctttccct gtctcacggc
    15841 acggcataaa aaaagaagtc ctgtttgcac agggcttctt tgaaagtact aaaaatttac
    15901 tccggcggga attttagctc gcccgtcttt gggggcgttt tatcgccata cacttcatac
    15961 atcttcatat aaatttcgcg gtctacaggg gtggcatcaa caagtttgaa accaatattg
    16021 aacatattgg ggtcgaactg atcctgcctg caccacatag ggcgggcctt cagaagaata
    16081 taagacttgc ttgaaatttc gccaagctgg tctatataca ggcgcatatc aatattaagt
    16141 gaaacgcttt gggagctatc gatgcgaaat ccacccgtgc ttatatctga gatatgcccc
    16201 agcactttcc ccgaagcctc ttctttgacg cgcatgtaat aattaaaatt tcttcgtgcc
    16261 aaccgtcgtt gttccgattc cattggagcc tcctgggtac taataaaaaa cgacaagtta
    16321 catttcagta tatacccatg aaagacctaa agcaatcaaa cttaagtatc gtttctcaag
    16381 atgtgcgtga gaatggatgc acccgagccg ccgatgtttt gcgccatacc gatcttcgca
    16441 tcagctacct gggtctgacc acattcacca cgtaattgct gaaccacctc aacaagttga
    16501 tagacacctg ttgcgccgac gggatgtcca cgggctttca atccgccacg agtgcagacg
    16561 ggcactctcc ccttggggct gatctcgttt tcaagagcaa gcttcacccc ttggcctctt
    16621 tcggcaaatc cgctggcttc caatgaaagt gctgccatga tcgaaaatgc atcatgcaac
    16681 tcaaatacat ctatatccgc gggggtcaca cctgccattt catacgcacg cctggaagat
    16741 tcgtaggctg cctgcagaaa tagcgggtcc ttgcgtgagt gaacggcaat ggaatctgtt
    16801 gctgcagcag aagcttcaac gacaatgcgc ggctttcttg caactttttc ggcggggaca
    16861 atcaccatgg cagccgcccc atcaccgatg ggagaggcat ccagcaaatt aatgggagtg
    16921 gccaccatgg aggatttctc gaacttttcc acggatattt tttcgtgcaa gcgggcatat
    16981 gggttgtgca tggcatttgc atgggcattg atcgaaaact gcgcaaagtc ttcatgcttc
    17041 caaccgtatt catgcatgta gcggcgcatg accagcgcgt tgatccctac aaaggaaaca
    17101 ccctgctcca cttcataatc agcgtcggcg gcagtggcaa gggcggctgt gacatctcgg
    17161 ccagccttgt ctgtcatttt ttcgacaccc actaccagcg ctgattcgat gtcgccggag
    17221 gcaacagcca tcagggcggc acgaaacgcg gcggcacccg atccgcaggc ggcttctatt
    17281 ttcaccgctt cctgtttcca cagaccgatc caatccgcaa ggaaggagcc gagttgattc
    17341 tgcccattga tcagggggga aagcatattt cccacaaaaa gcgaatccac tttttcgatg
    17401 ccagcatctt gaagggcggc aaaggcggct tcaccggcaa tttcacggag ggatttttcc
    17461 caatgttcgt cgattttggt ttgaccaatt ccaaggacgg caatttttct catactgcaa
    17521 attctaccgc attgccctat caagaactat ccaaaatgga aggaacaaat catgatttcg
    17581 ggtcgtccat ttttcattac aatctcaagg agaaaacatg ttacgctctt atttgattag
    17641 tttgttcgcg gccgctctat ttctggcggg ctgcgctcaa tcaactgcga cggcctaccc
    17701 aaaccctgaa actggtcaaa ccgccatttc catgcccagc ctgcaaggtg actggcggat
    17761 taagctgacc cattcaggcg gcatcatggg tttgctgcgg acaatggaaa tttcgtcaga
    17821 tggaagttac acagtgaccg acgaacgctc aagccaaact gtaacaaaaa ctcttggtcc
    17881 gaaagagttg caggaactta ccaggcttat ttcatctgca caatacatcg ctcccagcgg
    17941 acccaatgga atggtctgtg cagattgttt tgtatatgac ctggaaataa aaggaagcga
    18001 ctcaactttt accattcaat tgaacgatat cagcctgcca gactcaggga tggctcccgt
    18061 aatcgctcac ttgagagatc tgattgacaa ggccctaaaa tagaaatgga ccagactgaa
    18121 tggcgtgaaa agtttctgga agccgttgcc aattgttgca gcgaagggaa agaagctgtg
    18181 gaattcattc acgctcacaa agtaaggatt ggaattaaac cagcccgaaa aagcgtgggg
    18241 gcattttgga ctcttgaaaa acgggcttac ttaaattcgg tttacttttc accagagtct
    18301 tcacttgcca acccaaacgc ctgggtttta ctcatccatg aggtcaggca cttgcagcag
    18361 ggatggagta cagcgttatc catttatgga gaattggacg catggcaata tcaattcaac
    18421 acactcaaaa agattacctc cgccaaactc cctgaggcgg ttgatgaaat tctgtctctg
    18481 ccattgagta tggatcggga aaccttgggg cgtgcccgca aattgatgac ccaacatgca
    18541 gggaaagggt atggcgcaaa tctacttccg ctttacccga tccacatgga aataaaatat
    18601 tggatttccc cggattgata ttaatctacg cgacctcttt caaaaataac aggtattttt
    18661 aatgtactgg ctcaacacct gttgtatgct atgtacagat aaagaaagga ggttgcgata
    18721 gatacaccgc ctgaactaca acaaaatgga aaggtcgagt cgcgttgacc tggtccaagc
    18781 ccaaacttga gtaccagacg ttcccctaac gatataacgg ttgcaacttc gttcctttcc
    18841 aaaacataga gaacgcttga cggacgggta aactcccgtc cgtctttttt tgttctgagg
    18901 aaattcagaa tttcaccaac tacaaggcag aaaaagcctt ctgcatcttt tccaaaccct
    18961 gttcaagaat ttttcgcgat gtaccgaaat tcaagcggac atgccctgcc ccttcatttc
    19021 caaaaatctt cccttcgctg agcgccactt tggccttgtc tttgaaaaac tcgtagggcg
    19081 agccatcaat tgccgtttgg gtgaaatcca accagcccag gtatgttgcc gaaggcaccg
    19141 ttgtgcggac tccggggaaa ttacgggtga tatattccac tagaaaatca cggttgccag
    19201 tcaggtaggt ctgcaattcg gccagccatg gatcacactt gccagaaaaa gcagctcggg
    19261 cggcgtgcag ccccatgctt cccacgtgca ggcgcatgtg ctccatttcg gttgtgtaac
    19321 gttcacgcaa tgacttgttc gggatgatcg caaacccaca aaacagccca ggcacattga
    19381 aagtcttgga tggggcaacc agggtgatgg tatgctgagc gatctccgat gagatctttg
    19441 ccatcggggt gaatttattt ccatccaaca tcaattcaga atggatctcg tccgaaacga
    19501 tcaacacctt gttgcggatg caaatttccg ccatgcgggt gagttccttg cgcgagaaga
    19561 taattcccaa tgggttgtgc ggattgcaaa gcaggaagat gcctgctttt ttcacccgct
    19621 tttcgaacag atcccaatcg atttcgtagc tgatgagatt ccctttcaca tttttcacca
    19681 gcggaatatc cgtctgcgga attccaacat tattcttcac ctgatgaaat tcgttgtaca
    19741 ccggcgtttg aattgcaatg ccttttttgg gtgtgcaaaa tgcacgggtg gctactgaga
    19801 atccgctgac gatgcctgtt acagttacga ccgattcggg tttaaatttc caggaataca
    19861 aacgatccat gcgcgcggcg acggtttcct gcaaaacagc gccggggatt tcgtatccca
    19921 acacgccatg atccactgct ttgtgcaatg catccaaaat cggtttcggt gcggggaaat
    19981 ccatatcggc cacccacatg ggcaggatat ctttttcgta ccaggtccat tttgtgcgat
    20041 tgaaattctt tctacgatca ggtgtgatat tgaagtttgt catagatcct tgtgctgggg
    20101 tatgttgtca tcatgcccat ggtatgaaat gtggacacac caatgctgtg tcctattttt
    20161 tatacgcgac gatcatcccg tcacgcattg gcgtgagtga gacgatccaa tcgcggtctg
    20221 acgtaatctt tctggtgaat tcctgcacgc ccatcgtggc ggacgagaca tccttttcat
    20281 caaaaatacg cccatgccag agcatattat cgataataag caacccgccg ctgcgcaatt
    20341 tatttttgat ctcgggcagc gatgcagggt aggcgtgctt gtcaatatcg ttgaaaatga
    20401 tatcaaacgg accttctgtc tgacgaaggg tttcaaccgc ctctgccata tgaaactgga
    20461 caaggtcggc gaatcccatc ttaacgagat gtttgcgtgc cctcttcgac agttcctcgt
    20521 cccaaacagt gtgatgcaca acaccgccgc cattttccac gagcgccttc gcaaaccagg
    20581 ccgtggaata tccgtagccc gaccccaatt caaaaatgga ctttgcgttg atcatccgcg
    20641 cgagctgata gcaataatat ccgcacgcgg gcccgatgat gggaaaatcg tttttctcgg
    20701 cgtaagcctc catctccagc aattctgctt cacgtgatgg aacaagggat gcgagataat
    20761 cctgtaccgt ttgataatcc aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20881 nnaattcaag gcaacccgcc ccaataattg gtgatagaat accaaaggcc gtttggcctt
    20941 tttgaatgta cttatcacat gcaaggatct cgccctgaat gaaatataac ttccgcctgt
    21001 tttggcgcac cttctatcgc tcattctttg actcaaaaaa cactccagcc cgcctcaccc
    21061 gcaaaagaat caccttcctt atccttttct atctcgtctg gcccatcggc ggattgatac
    21121 actggttctg ctttgcgctt gatgacatcc tcttccccgc ctacaaaaat caccccatcg
    21181 aaaaacctct tttcatcctc ggcaatttgc gcagcggatc gaccttttta catcgcctgc
    21241 tctcacgaga cacagagacc ttcaccagcc tgaccacatg ggatatctac ctcacgccct
    21301 ccgtgaccca gaaaaaattc acacagctta ttgcaaggct tgataacaaa tatcttggcg
    21361 gaaccttaca caagctgttg tacaaattcg acgccgccac cctcggaaag atcaaaattc
    21421 atcccatctc cttctttcaa cccgaagagg acgagaacat ccatctgcat atctgggacg
    21481 gctacttcgt caccttcctc ttccccttca tggatgaatt ccccaattac cagcatttcg
    21541 acgaagccct cagccctgaa cataaaaaac gcatcatgac cttttaccgc tccatgatcc
    21601 agcggcacat gtatgcgaac ggcaaaaaat atttcgtggc caaaaacccc gccttcagcg
    21661 ccaagatcga aacgctcgcc gaattcttcc ccgaagcccg catcatctat ctcgcccgca
    21721 acccgctcga catgctgccc tccaccatct cgtggatcaa ttatgccagg cgtcaattca
    21781 ccgacccagg cgaaggattc ttttacattg aagaaatcgt tgacatgacc aagcactggt
    21841 acagtcaccc gctccaatat ttagactcgc atccctcgcc ccgccacctt attatgaaat
    21901 acgatgacct gatccagcgt ccagagaccg tcatccgcgc cttctatgaa caattcggct
    21961 atcccgataa acctggcctg ccagtcatca ttgaccaggc cgtgaaagaa actctcacct
    22021 ttaacagcga tcacgcctac tcctacgaaa gcatggggtt tactcgtgaa cagatcgttg
    22081 ccatgtaccc tgaggttttc aagcgcttca actttgagac ccgcgaacca ttcatcgaat
    22141 cggaattaat gaaagtggaa agaaaaaaat agcgcatctg ccaaaacgaa ctcgcccgcg
    22201 aaagatcaat ttcggggcga gttcgttttt aagcgcaaat tgggcacaaa atcaattatg
    22261 gaaaatataa ctgaagcaaa cggccagcct cctggccgta cgggccagcg gggtccatat
    22321 ttttcacttc attcaatttt tcaaaagccg catcccgatc acccattgaa agataggtaa
    22381 gggccagatg aatatgtgcc tgcaaatgat cggggaaacg cgttatcaaa tccacaaaga
    22441 tattttcggc gttggcaaat cttcctgaag agagataagc ccaacccaat gcatccagag
    22501 tgagcgggtc attcggcgca agttccactg ctttttctgc ggcaggcaac ccaatgtctt
    22561 ccatgtgaat ggtattctca gcacaaaaaa cagccagcaa acgccagtag gttgggtcat
    22621 cgggcgcaag ctcggtggca tgttgatagg ccgccaacgc agaaacaagg tcaccgcgct
    22681 tgatgtaggc gttcccgatc tctgcctgcc aggcaggatt ggtcgggtcg tattcggcag
    22741 ccagcaaata ctcagccagc atttgtggat acttctcctg acgccgccag tacaacccgc
    22801 gcaaagcacg gaccgttaca gactcatggt caaggaggac cgcctgatcc aattcagcca
    22861 aaccgatgtt ctccgcatca ctctgcgctg attgctgctt cgcttcgcca agccacgccc
    22921 atgcctcggc attttcagcg ttaaacgcaa tggctttttc aaatgcagca accgccaaat
    22981 cccattcctg cacaaggccg agggcacggc caatgtgaat catttgttcc gatgcatcgg
    23041 gttgggtggc agataaattc aaggcggtcc gcaatgtctg caccgcagga tcaaattcag
    23101 ggttaaggga ggaagcgagc atcaaatccg tgagcgcctg ctcgggttca aggacactca
    23161 acaagaggcc gagccgataa cgggcgtaga catccccgcc gtttggatct aaacgcaatt
    23221 gcgcttccaa agcggcgcgt tctgcgggcc agtctttttt ttgacgatag gcaaaggcca
    23281 gcaaaccatg cagcatgggt gcctcgtggg tttgcaaacc gcgttgaagt acctcgatcg
    23341 ccgaatccac ctcacccagc gacaggtacg aggaacccag ccagccccag ccttcttctg
    23401 aaagtgtgtc accagcctga tctaaatata tgatggcttg cgccgtatcc ccgccttttg
    23461 ctgaagcaat gcctgccttt tcccacaacc ctgtttgcca gaaaagaagc cttgcagaac
    23521 gttcgtaggc cttggcggct tctgcataat tttgggctgt aaagtgggca tcggcttctt
    23581 ttagcgaatt ctggcctgcc gcgacaaatg caaaaaaagt ggccgcagca acgaccacga
    23641 tgacgatggg gatgagaatt tttttcttaa gcattgcaat ggtaattata cgtgagcgaa
    23701 ccatattttt ttgaactgga agtataataa tgtcgacatg agccctttca ccagtcaaat
    23761 ctttgaatta ctcacagttc ccatcaacaa cctgctttat tacatcgtgc tttcctacgc
    23821 ggtggcaagt gcgatgcttt cggcatttat ccattggcgg gccagtgaat ttccgcaagc
    23881 ccgccgcgcg ctgatcggtt tgggggtatt gcttgccgca caggtaacca tgttcctgtt
    23941 cagcgggttg ggctggcagg gattgatcga cccaaagata ctgccgccga tggaccgtgc
    24001 tttcattatc tttggaatta tttggatcac ctggctctat gcctttcctg agcccagccg
    24061 tccagccgat gctgctgcgg ctttgttgag cgttttggtg ctgaccgcgc ttgggctgag
    24121 tttgcttgcc tggcaacccc agatcacaac catcacctac aaccaaacct ctgatgactg
    24181 gttctggcaa attgggtcgt tagcggttgg gttgatcggc atcgccatca tgttcgtccg
    24241 caagccagat ggcatgtgga acggcattgt tctgcttggc ttggggatat tggggcatat
    24301 cggtcatctg gcttttccca ttgacggaaa ttactcgggc gttgtccgca ttgcttatat
    24361 ggcggcctac ccgattctgc tgacattacc gttgcgtttt tccacaccca gttctgcaaa
    24421 cgaatcacca gcacgacaga ttacccaaaa acaggacgaa aaccttgggg aacgcagacg
    24481 gtacagcaca gacccgaaga ccttccatgc catgctggca cttgccgcag aaaccaaccc
    24541 cacgaaggtc agtcaggcta tcacccgtgc catcgcacag accatgctct cagatctgtg
    24601 ctttatgatt tacatcaacg ataacagcaa ccaaatgtcc attgcaggtg gttacgattt
    24661 gattcgtgaa gacgcattgg aaggcggcac gctgaacaaa ggctccattc ccatgctggc
    24721 gaattccctc cagcgcggac gaccgttgcg aatgccatcc agcagcacct ctgcggacat
    24781 taaggggttg agcgatattc ttggattaac caaccccggt catcttttga gcgtcccgat
    24841 tctcaccgct gaaaaggaat ctctgggcgg catccttttg ctttcgccct actcagaccg
    24901 cacatggaca gctgaagatc aggcctacct ttcaaacatc gccacctcac tggtgccgat
    24961 catccaacgc ggacaaaagg tcaacagaat ggaagcgcag agcagcgaag caattcgaag
    25021 atctgccgac cttgagcacc gcgtacagga attgaccgaa gaattacaca acgcgcagac
    25081 ggagatccaa aaagcaggct ctgtggaaat ggcatccctg cttcaggcac aggaagaatc
    25141 gcagcggatt attgaacagt tgcagcttga aaacgcggaa ttgcgcacgg gaaagagttt
    25201 gcaaacaggc gcagcacccg cttcatttgc ctccggtcag atcgagcagg aattgaaaac
    25261 caccctgcaa gaggtggccc gcctgcaaaa ccaactcatg gaagccaata tgaagacccg
    25321 cgagttggaa aaggggcatg tgacggtcaa gagcacagaa caggccgaag tggttgcctc
    25381 gatcgcacag gaattgcgtc agccactatc atccattgtc gggtacaccg acctgctgtt
    25441 gggtgaatcg gttggaattt taggtgcgtt gcagcgcaag tttgtggaac gtgtcaaatc
    25501 ttccacggaa cgcatccgca gtttgaccga tgacatgatt cagatcacca ccctggaagc
    25561 agaattgaac gacctcaatc ctgaagccgt ggatctgaac gcgatcattg acaacgccat
    25621 gtcttacacc agtacacagg tgcgtgagaa gaatatttcc atgcatctcg aactgccaaa
    25681 acaacttgcc cccatccacg cagatcgtga agccttacag cagatcctca tccacttgct
    25741 gcaaaacgca ggggcggctt cgcccttcga aggcacggtc aggctcaagg tccagacccg
    25801 aaacgaggag aacacagagt tcatccttat tcaagtaacc gacagcggcg gcggcatccc
    25861 agccaaagaa ttgccgcgtg tgttcacccg tctctaccgt gcggacaatg tcctcattca
    25921 aggtgtggga gatacggggg ttgggctttc catcgcaaag actctcaccg aagcacaaca
    25981 tggccgcatt tgggttgaaa gtgaacagga cgtgggctcg acctttagtg tgttgctacc
    26041 catcgcgggc agcgcagaga ccaacccgaa aaccaagcct aaaggaaaga aataatgcaa
    26101 agcatgcgcg acctcagaaa cgcgcttgtg gtagccatga tctcgatcgg tttgacggtc
    26161 gggtcattat ccatttccct ggtggaattt gcccctcagg cagttcccag tgccacaaat
    26221 cctttgcaag ttgtcccgcc actgctgacc gccacgccca ccgtcgtccc ctcaatgact
    26281 gcgacccttg ggcttgaatc gcctacgccc agccccacat ccaccttcac agccactcca
    26341 ccgcccccca ccacctgccc tccgcccaca ggctggaccc agcttgtggt tcaagcgggg
    26401 caaacgcttg ataccattgc ccaacaatac cgcaccagca aggaaaacct gcgtgctgga
    26461 aactgcctca tcagtgacaa ccttgtggca ggctcgatca tgtacgcccc tgccgtaatg
    26521 accagcaccg tccccgcttg tgtgccaggt gctgctggtt ggaaaaagtc atacactgtt
    26581 gtgtcaggtg atacgcttta cgccattgcc accaatcatt ccacaacagc aggcttgcta
    26641 aagagtgtaa actgcctgag cctcgacgat atctacccgg gcaatgtttt atgggtgcca
    26701 gatgtgccca cacgcacacc caaccccact ccattacctg gcagcacagt gaccccgatc
    26761 ccgacccagt tgttgacgga aaccgccctg ccttacactg ctacgacact ccccagcgta
    26821 acgccgacgg aagtcccagc gactcccact ccgaccacgc ctgccacaga tacaccgaca
    26881 actgccccca ccctgacggc aagcccaacc ccatttcccc cgtaaaaatc acccctacgg
    26941 atgatctttt cgcgctgatt caaacaacgc ctacctagat gaaatccaat tccgcctttt
    27001 cgaccatcgc gcttctggcg tttttgctct cagcatgtgg cacacccgcc gaagcggttg
    27061 tgcaaacacc cctgccgttc gtcttaatca catcggcgcc aaacccatct cccacaccaa
    27121 cacctttcca accatcgctg aataccccta cacagtctgc aattctgtct gcagcagtgg
    27181 atacatccac acctgtaccg acggtcatcc tgcccacaga aacgccttcg cctactttaa
    27241 ttccacccac cgaatcaacg gccacagtgg atttcaacca gcttttcccc acagttgctg
    27301 ccccgcccgt ggttgaagta gtggccgtca cacccaccgc cctgcctccc ctcaccgata
    27361 acgaaacggt caacttcctg ctcatcggct ccgacaaacg cccaggctcc tcctaccgca
    27421 ctgacacatt ggtcattgcc atcgtctggc caaaggaagg tcaggtttcg ctcatctcca
    27481 tcccccgcga tttgtggatc tacatcccta cggtgggaat gcaacgtatc aacaccgcct
    27541 accaaagcgg agagatcggc ggctatgccg gcggcggcgc tggcttgcta aaagacacta
    27601 tcacctacaa cctcggcatc cgcattgacc ataccgccat ggtcgagttc gacggattcc
    27661 gccgaatcgt ggataccctt ggcggaattg aagtccctgt agcctgtgcc tacaccgatt
    27721 ggagactgat cgatccctcg ctcgacatgt acgatgaaaa caactggtgg ctctacacgg
    27781 ttggccccgg gcaggttcac atggatggcg atcttgcact ctggtatgca cgttcacgct
    27841 ccaagtcttc cgattttgac cggggccgac gtcagcagga agtactgcga accattttcc
    27901 aaaaagccct gcaaactgac accttcagca aaatccccca gctttacgaa gacttctcaa
    27961 gcaccatcct caccgatatg ggactggcag acatgctgct catgtcacca tacgctgtca
    28021 actttaccaa cgccaacatc cgcggctatt tcatccgccc gccctacgtc acttcgtgga
    28081 caacccccgc gggcgcggcg gttttgctcc ctaatgaaac cgagctcaac caaatgctcc
    28141 tcgaagccac cacactttcc acttttgccg aacagcgcga aaccatcacc gtcgaagtgc
    28201 aaaatggctc cccgttcgac accatggaaa gcctcgctgc ctcacgcctc aattacgcag
    28261 gctatcaaac catcctctcg aatgccgacc gccgcgacta tgccaactcc gtcctggtgg
    28321 atttcacccc cgggcaggat gccacccaac gagaagcgat catcgccacc ctcaacctgt
    28381 ataccgcaaa tgtaatttcc ctccccgatg caaacagcac ggtgcaatat cgagtcatcc
    28441 ttggcagtga ctatcaggcc tgtttccagc cacaagacct ctcacattga aaaaaaaacg
    28501 gcatccccgc cgccaagaac ggattaaatc aacattacag tttcaatctc ggcagttgac
    28561 aacctctcct atattcgcta aactatacga aaagactatg cgctagtacg taaggaagac
    28621 cttatggcaa aaacacctga ttccgctcat atttcagcca agaccctgct ccctgccgcc
    28681 atccaaggct gggaaatgtt catgctcgat cagggacgtt ccacaaacac ggtcaaggct
    28741 tttctttcgg atgtccgcct gctgactcaa ttcctccccc cagaccaatc catcgggtcc
    28801 atcacgaccg aggatctcaa tcgcttcttc acatggatgg aaaaagaccg cggcattcca
    28861 tgcagtccca agaccctttc acgacgtatc acctccgtca agtcattttt ccgttggctc
    28921 caccagcacg gttctctggt tcttgatccc gctgaaaaag tgttacaacg ctctgccatc
    28981 agcccgcttc cgcaagtact caccgacgac gaatacgagg cagttttgct ggcagcagac
    29041 cgtctccgca gggcttccaa accagatgcc cgctcctaca ctttggtata cctgctgctc
    29101 acgacaggca tcaaaaaaag cgaatgtctt ggcatccaca tcaaccacat tgaccttgaa
    29161 gccaagaatg gaccccatgt attcattcgt tacgccagcc ctgccaaccg ctacaaggaa
    29221 cgcaagatcc aactggcaga agactggctc gaagcctata aagaatatct cgcccaatac
    29281 caacccgttg accaggtttt tgcatggtcg ccgcgcaggt tggaatacct tctcgaagat
    29341 atcggaaatg aagctggcct caccaagcac ctctccttcg atatgtgccg ctggacctgt
    29401 gccctgcgtg attatcaatc aggcatggaa gcagacaaca tccgccaaaa gcttggtgtt
    29461 tccaaaattc aatggcgcga gttgttcatc aagatcaggc aattgaacgg agagataaag
    29521 taatcgtcca ccgctaaaaa tacctcaaga ataaaaaatt cccgctgact agagcgggaa
    29581 ttttttattc ggttattttc tcaagtgggc ccgaaacaga aaaatatatg gcggtcttgg
    29641 tggattgtgt cagacgatag gaatgtgcag ggcgataccc cggcacccaa atgatctggt
    29701 cgccagcgca caacaagggc caatgatctc tggcacgctg gggcattttc tcgttgacaa
    29761 agaaatcgga aagcttttgc gaatgacctt ccaaaccaag cggttcgaag gtatctccct
    29821 gatggcggac tcgcaattca agcatctcgg gaacccgttc ggcatccaac cagatctgga
    29881 acggatcttc attcctctca gcctgctcgc gtaccaatgt gggaatatgc caacgctcgc
    29941 agctaaaccg ccacccacct gcaagttcca cctgcccagg aattgccacc tgaacggggg
    30001 ttgtgctggg catttgcggc cagaggttga aggggagttt cgaattttgg gtgcaaacgt
    30061 acaaaacacc cgcttcgcgg aacatgcgca aaccgccttt caaatccacg ctggaggagt
    30121 gggaacggtc tttgatcagg tcggttgccc gttccagggc ggaaaagttc acatccagct
    30181 ttgggcgcag aatctgcatg gcatgtttga ccagattccg ctggagtccg atcgagcggt
    30241 tggcaagcag gtcggcgtca aaggcaatgg tatcctcttc cacactggta acaacttcct
    30301 gccatgcatt ttcgagcgtt tccatcagga gagagtaatc actcttcagc gattgggaca
    30361 tgcgcagcac gacatcgcgg aattttgggt tgtagctttc gaggcttggg atcaaaaggt
    30421 ggcggatacg gttacgctga aaattgatcg aatcgttgct tgagtcgtaa tgtgggcgca
    30481 aaccgttggc cgcacaaaag atggtggtct cttcacgcca ggtgttcagc aacgggcgca
    30541 caatgggaat cttggggtca aatgttttga tgatggagcg atgcgacatt ccctttaaac
    30601 ccgagatgcc gcttccacgc aggaaatgca tgagaatggt ttccacctgg tcatctgctg
    30661 tgtgacctac cgcgaccgcc tgtgcgttcc tttcacgtgc caatttaaac atgaagtggt
    30721 atcgcaaatt acgggccgct tcttccagtg acagtttttg ctgatctgca taagcgcgca
    30781 catctgcgcc atcaatgatg caacccagca tcaggcgggc ggcgatcttt tccaccatat
    30841 gggcatcctg cccggcttcg gggcggagtt ggtgattgaa atgcgcaata atgatggaat
    30901 agcccgcctg cctcaaggtt tccatcaagc acaaactatc cggcccgcca gagaccccga
    30961 caatgatcgg gcggtcttta acgaggccgc attcgtcctg cagggtggcg tccatgtttt
    31021 ccagcatcat atctttactt cgctttaatt gtgtgcttta caaaaaccac agaggggttt
    31081 ctgcatcaaa gccacggggc taaatttgat ataactccac caacacgcca ccagtggatt
    31141 cagggtggat gaaggcgtat ttttttccgt caaccgcggt gcgcggctct tcgttgatca
    31201 agcggatgtt cttcgccttc aactgtgaca tcattccttc gatgtcatcc acttcaaggc
    31261 agaggtgatg catgccgggt ccgcgcttgg ccagatactt ggcaattccc gaatcgtcag
    31321 aagtgggcat caccaattcc acttcagcgc ctgcaacggg caaaaaagcc acctgcgatt
    31381 tttcagcagg gacatctcgt agttcatgca attgaattcc aagcgcatcg cgccagaaca
    31441 aaagggattt ttccatgtcg tccacgacga ccgcaacatg attgacagct ttgatttttg
    31501 gcatagtgtt cctcaacggt tggattttac ttccgattaa cgatttcaag cgccacggaa
    31561 atgacctttt ccacccacat ggagtacatc ttccctgatg gatgcaaacg gtctggggcg
    31621 agtaattcaa aatcatccat tgccatgcgg gagatctcag tgacatccac ataatgtgcc
    31681 ccggcggatt tggtttcttc aaggttaact gcattaaatc cgtcgatttg caggctgatc
    31741 ggttcggttt ctcgggtggc ggcaaagggg gtaaaccccc aatcgggaat ggataacaca
    31801 atcacatgct gtggatcgcc cccggcgtat tcaatggcct tgccaagcat aaagcggaaa
    31861 tcctcccgat aaccatccag cggataaccg cgatactggt cattcacccc gatcaagagt
    31921 gtgacgagat cataccttcc ttcgggcggg gtgacttgaa tgccctccca aagttcatcc
    31981 acggtccagc ctgtacgggc aatgatggtg gtctcggcat gaattccctg ttcttccaat
    32041 ttggcagcaa gttgattcgg ccaacgctcg ctttccctca cactttcacc gatggtataa
    32101 gagtcaccga gagcaagata gcgaatttcc atttttgttg cattctccac ggttggttct
    32161 aaaggttggg aacaggcaaa aagaaaaaca gacagtacaa atgcaatttt tctcacaagg
    32221 caagtatatc aaaaatcgtc cctgtcaaac tggcagggac gatttttttc gaatgggtga
    32281 gagaagaact aaagtgagcg gaccttcagc cacaagctcg gaattcggct gatcttaccc
    32341 atggcagaaa ggctggcgcg gcgattgaat acgttgtaat cgtgtttttc gatatcttcc
    32401 aaaataccgc aataaaaagt ggatgccgct ccgatggcta gctggccttc acgctcgagc
    32461 attttcacgc cttcccagga ttcttcatat agctggcggg tacggtcgat ctggaatttc
    32521 atgaactggc gccaacgatc tgtcactttt ccttcggcaa tatctgcttc ctgaataccg
    32581 tagaagacaa gttcttcgcg tggcaggtag atgcgcccgt tacggtaatc ctcccccaca
    32641 tcgcgcagga tgttggtcat ttgcagggca acgccaagtt taatggcgta gggcacagca
    32701 tcatggccct tgaaaccgac aatgtacatg ctcatcaacc ccacagtgga ggcgacaccg
    32761 tagcaataag tggcgaggtc ttcaaaggtt tggtagcggg tttgggaaag atcacgcgcc
    32821 acgccgtcga ttaattgcag ggcataatga tgcggaatat ggtatcgggt aagggtatct
    32881 gcccaggcgg aggcaatcgg gtcatcggtc ggggaagaag cggtctttac aatgttgcgc
    32941 caataatcga gttcaaagtc gcggtctttt ttcaactcga cttcatccac aatatcatcc
    33001 acggttcggc aaaaagcata caaggcacgg acggcggaac gtttttcttc aggaagcagc
    33061 ccggaagcca ggtagaaaga tttggaatgt tcactggtga tctttgcggc ctttttatag
    33121 gcggcgtgca gttgggcatc tccagcccag tacgaaaaga agggacgggc agaagtgtgg
    33181 gggaagtgcc ccgcgagggc gatcagttgg ctttcccaat tcgcttgtgc ttgcatgttc
    33241 tctcctgttt ttagtttctc tgagtagggc taaaacaacg tgctgatgaa aatattttac
    33301 aggatatgtc tattttgtca aggttttgca aagtattttt caagcaaacc ttgacaaata
    33361 ctagacaaag catataataa cctaagacgt tttttgaacc tcaaggagaa aattatgcga
    33421 aaacagaccg cagtagtgat tggtgcaggc atcggtggaa tcgccactgc cgcccgcctc
    33481 gccaaaaatg gatacgacgt gaccgttgtg gagaaaaatg acacccccgg cggacgttgc
    33541 aaccagatta ttcaagacgg ccaccgcttc gatattggcc ccaccctgtt cctcatgcct
    33601 gaggtatggg aggagacctt cgccgcgctc ggcgaaaaac tgagcgacca ccttgacctg
    33661 cgccgtattg atccaactta caaggtccgc tttgatgatg ggctgcagct tgaattgact
    33721 tcgaacatcg gtgatatgca aacccagttg gaaaaagtgg aaaagaccgc ctttactggt
    33781 tttttgaatt acatcgctga aggcagcaaa cattacaaaa tatccctcga aaagttcgtg
    33841 ggacgcaatt tctacaatat ttttgagtac ttcagccttt ccaatttgcc gctgatcttt
    33901 caactcaagg cgttgggcaa gcattacacc aacacaggtc gtttcttcaa agacgaaagg
    33961 ctcaaagccg ccttcacttt ccaaaacatg tatctgggcc tcagcccata tgatgcccct
    34021 gccacctact ccctgctgca gtacacagaa ctggcagaag gtgtttggta cccgatgggc
    34081 ggcatgtatg ccggcattca ggctctgacc cgcatcgctg aaaagttggg cgtaaagttc
    34141 atctacgatg cgcctgtgaa gaagatcaaa actggaaaat caaaagtgga tggcgtgatt
    34201 ctcgaagatg gccgcgaatt gaccgctgat attttcgtcg gcaatgcaga cctgccctac
    34261 atttacgacc agcttctgcc tgaccgcgcc gaagcgaaaa aactcatcga cgaaaagctc
    34321 tacacctgct ccaccatcat gttctattgg ggcgtggata aggaatactc ccagatcgcg
    34381 catcacaacg tcttccttgg cggcgaatac aaagcctctt tcgagaagat cttcaacgaa
    34441 cacaccctgc ccgaagtgcc atcgttctac gtccatgcac ccaaacgcac cgacccagcc
    34501 gccgcaccgg aaggacagga caccctgtat gtactcgtcc ccgtcggtca ccttgacgcc
    34561 cgttctgcac aggattggga tgccctggtt gcccgcgccc gccgctacgt gcttgaccgc
    34621 ctcgcgaaag aaatggacgc ggccgatctc gaagcgcata tcaagtttga gatcgtgtac
    34681 cagcccaaaa catggaagga gcgtttcaac ctcgtcaacg gctcagcttt tggcctcagt
    34741 cacaatttct ggcaggtcgg ttacctgcgc ccgcacaacc gtcacaaaac ctacaaaaac
    34801 atgtactttg caggcgcatc cacacacccg ggcacaggtc tgcccattgt cttgctctca
    34861 gcccgcctga ccaccgaacg cattctcaaa gaatttacgg gccaacaagt agaaggctac
    34921 aaactggaaa aagcagttgc acaagggtaa ttcggcacaa agaaggtaca atagccgtgc
    34981 aaaacctttg cagctcaccc aatttcagct tgtaaccgga gttcatcatg tccatcagca
    35041 aaaaccccgc ctttaactta aaagtcgtcc tgcaagaaac aggcatcgcc gccgacacgc
    35101 tgcgagcctg ggaacgcagg tatggcatcc ccatgccaca acgcaccgca ggcgggcatc
    35161 gtttgtattc gcaatatgat atcgagacga tcaaatggct gatggcccga caagccgaag
    35221 gactctccat ctctcgtgca gtggatctgt ggaatgaaca caacgcttcc ggcgtcgacc
    35281 cgttggctgg ttttatacca acaacaaaca tcgccccgtt ggcgactcct gccattaccc
    35341 tttcagccga tacaagtctt gactccatgc gggcgcaatg gatcgacgcc tgcctcaatt
    35401 tcagtgaagc cactgccgag caggttttga accagggctt ttccatgttt ccagtggaag
    35461 tcatctgcac cgaagtgctg caaaggggaa tgtctgagat cggcggtctt tggtacgaaa
    35521 accgcgcatc ggttcaacag gagcattttg cgtcaggcct ggccatgcgt cgactcgacg
    35581 ccctgatcag cgcttcacct gccccttctc tcaaacagac cgtgttggtg ggatgccccc
    35641 ccaatgaatg gcacaccttc accccgcttt tgctctgcct gttacttcgc cgccgcggct
    35701 ttggcgtcat ttacctcgga gccaatgtcc cagcggagca atttgaagaa accgtttcca
    35761 gtgtgcatgc caatatggtc gtccttgtgg ctcaaacgct cattaccgcg gccgccttac
    35821 attccactgc actttccctt gtcaacatga aaataccagt cggcttcggc ggccgtatct
    35881 tcaatattca tccagccctc aaagaataca tccccggtca ttacctcggc aacacagtga
    35941 ccgcctccgt tgagcggatc gaacaacttt tgaagggcaa ccagtccaag gttcaggcaa
    36001 aaagcgccac cgaagctcat cacgttgcgt tggagcattt caattcccag cgtttacaca
    36061 ttgaaagcag catcaaggaa atgcttccgc cgttcgtcgc caaagacatt gacaccggca
    36121 ttcacttcct tggcgataac atcgccgccg ccctccaact tggtgatatg gagcatgtca
    36181 ccgccgaatt ggaatggctg aaagtgctca tgcaatcgca ccaacgcccc gccagcgaac
    36241 tggcggattt catgcacaat tacgccacgg ctgtggataa gcacatcaat ggccgcggtg
    36301 aaccgatcaa agtctggctg aacgaacaag ccagactcac ccagttatga caaaacaaga
    36361 catcctttca accttttacc ataaactttt acctctattc cccccgcgga ccgaaggcta
    36421 cctcaaccgc ctcaccgcgc aggatttaca gcactccaat tttcgttaca tcttttccgt
    36481 gcaaagcatg atcgagatca gtcacatgat cgaagactcg gtcattaaca cgcccggcgt
    36541 ggcggagacg cacgtctctt tccaatattt ttcacgcatt cctgctcaat tggagcaata
    36601 caggaaagtg tcagaagcat ccactggttt gtggttgtat ggtgtgccag atgcacccct
    36661 gccccagctt tcccgcacca tcggcgtcag cacagatggg actccactgg aaacctattg
    36721 gtttgtggtt gcctatggcc caggtcttta cgccaccctg ctcgcagaag agatcacccc
    36781 ggaggaaggc caacgcatgt acgaaggttt ttataccttt gaaccagaga cagcctttca
    36841 agtggtttca ctgctgcacc aaatgttccc catgcaggta ccggcgccga ttgcgcctca
    36901 agaccaggag cacgcctgac atgaacatac gcatcgtcac agacagcact tgcgatctgc
    36961 cagatgaagt cacaaagcag caggccatta cggtgattcc actgaacatc aatattggca
    37021 atgacacgtt ccaagatggc attgacctca cccgtactga gttctatgcc cgcctgccag
    37081 aatattcccc ctcacctaaa accgcagcgc ccgggcctga tgttttcaaa aaggaatacg
    37141 aacgccttgc tgatgaagga gcgcgatcga ttctctcgat acacatctca gagacattaa
    37201 gcgccacgat caactcagcg cgcaccgccg cggaacaatt cacccgcatc ccggtcatcg
    37261 tgctcgattc cagccagctt agccttggca caggctttct ggttgaaaaa gctgcgatca
    37321 tgggcgcagc cggggaaacg atggagatga tcgtctcaac cctgcaaaaa atgatgaaga
    37381 ggacttacgt ctttgcctcg ctcaagacac ttgaatacct ccgccgcagc ggacgaatga
    37441 atttcgcgct tgccagtttc ggcgaacttt tacagatcaa accgctgctg cacatgcatc
    37501 ttggaaaagc caccgcccac cgcacgcgca cccaaaaacg ggcaaccgaa cgtctgcttt
    37561 cctggctgaa tgaatatgct ccctatgaaa aactggccgt cttgcatgca ggggcacagg
    37621 atgaagccga ggcactgcga gaaaaagtac gctcgttcct gcccaatgac gatgtttcca
    37681 tcatccagat caccccggtg cttggtgcgc acctcggcat cggcgcactg gggtttgcct
    37741 gcatttcaaa ggaataagct catgaaaatt gcaaaactca tttccattct cggcattctt
    37801 gccatgaccg ccgctctcgt caacgggttt acccgcggag acttcttcgg cgagggcggt
    37861 cagctggccg ccatgccgtg gggcattgtt tcacttgtgg acctgtacac cggcttcacc
    37921 ctcttttcag gctggatcat tttccgtgaa aagtcactgc ccgtcgccat tgcatggaca
    37981 gtagccatga tggtcctcgg cttcttcgct ggcagtctct acgctttcat tgccctgcaa
    38041 accagcggca atgactggcg caaattctgg ctcggaaaac atgcctgaaa aaacactcgc
    38101 gccacagggc aaggggcgtg aacttcttga agaattgcaa acggtctttg caggccgcaa
    38161 ttccttcatg gacgcgatcc ttccgccgat tctctttttg gcggtcaatg gcttggtgaa
    38221 ttttcaagcc gccatgtgga gcgcactggc ccttgccatc ttcttcgccg cgtttcgcat
    38281 cgttcgaaaa cagtcgctga tgtacgcgct ggcaggagtg ggcagtgtgg cggccgccat
    38341 tggaattgcc tggttccttg gcaagtctga gggatttttt ctgcccgggc tcattagcgg
    38401 aagtgtgacc ctgttcctga ccattttgag tctggtcatc cgccgcccca tggtggcatg
    38461 gaccagctac ctggcccgcc gctggcccct ggaatggtat tggcatccgc ttgtgatgcc
    38521 cgcttacagt gaagtgactt ttgcatgggc tgtctttttt gccatccgtc tgctttacca
    38581 gttcgtattt tttcaagatg ctgacgtcaa ttcgctggcg gttctcaatt tcatcacagg
    38641 ctggcctgcc acagttttgc tattggtagt gagttatctc tatggcacat ggcgactcgc
    38701 ccaactgcgc ggccccagcg tacaggaatt tctcgacgac tcccccgccc cgtggcaggg
    38761 tcagcgacgc ggcttctaat caactttata aaaggaaaat cctcaatgaa aacaacagat
    38821 cgcaacgcac tcatcgcatt tcccattttg atattgatcg gtgctctggt ggctctggct
    38881 ggcagtcagg gcggcgcttc gatcgccgga gttccgttat ttgccgcatc ggttgggctg
    38941 gcattcttaa ttcaatggct ggttttcatt cccgcctacc tgatgcagac tgagaaattc
    39001 ttcgatctga caggcagcct tacctatatt tcggtcacca tcattgcagt gatttttagc
    39061 ggaaccatgg atggaaggtc cgttcttctg gcggcgcttg tggtcatttg ggcggttcgc
    39121 ctgggaagtt tcctgttcaa ccgcattaga aaagcaggca aggatgaccg tttcgatgaa
    39181 atcaaaccct ccttcattcg ttttctgaat gtttggacca ttcaaggtct gtgggtgact
    39241 tttacgatgg cagcggctct ggtcgccatt accacaagcg ccaaaaaggg tctggatgca
    39301 tttgccatca ttggccttgc gatctgggtt ttcggatttg cgttcgaagt tgtcgccgat
    39361 tcgcaaaaaa gccgtttcaa tgcaaaccct gccaataaag gcaggttcat tcagacaggc
    39421 ctctgggcac gttcacgtca tcccaattat tttggcgaga tcgttctctg gctgggcatt
    39481 gctgtgatcg ccattcccgt tttgcagggc tggcaatggg ttgcgttgat ctcccccttg
    39541 ttcgtcaccc tgctgttgac ccgcgtcagc ggtgtgccac tgctcgagaa aaaagccgat
    39601 caaaaatggg gcgggcagga agattacgaa gcctacaaaa agaaaacccc cgtattgatc
    39661 ccgcgtttgt aattcaaaca gaaaagaaac atgatcattc aaatcttgca aatcatcgca
    39721 gccattgcca ccatcctcac agggttggtc tcgctcttca agccagagtc agtgacgggg
    39781 tttacgggcc tccgtcccga aggcggacgc ggcattaccg agatccgcgc cgtactgggt
    39841 ggattcttcg tcgcacttgg cgctgcacca ttgatgctta attcgcctgt cgcctacacc
    39901 atgctcggca tggcctacct ggttgtaggc gtggtgcgcg gcgtttccat gtgggtggat
    39961 aaatctgtca cccaatccaa catcatcagc cttgtggttg aaatcatttt cggtgtgatc
    40021 ctggtggttc ccgtatgagt cgtgctgaac tcaacaaacc cgcccccaat ttcacattga
    40081 ccgatttcaa cggcaatgag atcagcctca cagattttca cttgaagaag aacgtcctgc
    40141 tcgttttcaa tcgcggcttt ttctgaccct tctgccgacg gcacatggcg cagttgcgcc
    40201 aggattacaa caaattcaca gaacaagaca ccgtgatctt ggtggttgga cctgaaaaca
    40261 gtctggcatt ttcgttgtac tggaaaacca attccctgcc attcattggc ctgcccgacc
    40321 ccagccacac ggtcttgaag cgctacgggc aggaagtaaa cctcttcaag tttgggcgca
    40381 tgcccgccca ggtcgccatc gataaaaatg ggatcgcccg tttcgtccac tatgggcatg
    40441 acgcacgcga catccccacc aacgaggaaa tcctcgaatt attggaatcc atgaacaaag
    40501 aatcgattac atcgtaaaag gaatttatga acaacaaacg cctcttttca atcatcctgg
    40561 tcgtatttat tgacttgctc gggttcagcc tgatcctgcc gctcctgcca tattacgcag
    40621 agactttcaa tgccaacgaa accatcacag ggatcctgat cgcctcctac gcagtgatgc
    40681 agttgatcgg cgcgcctttg cttggccgcc tctcagaccg cttcggtcgt cgccccgttt
    40741 tgctgatcag cgtcttcggc accttcatcg gctttttgct gctcggcttc gccaacgccc
    40801 tgtggatgct cttcgccagc cgcattttgg atggcttaac aggcggcaat ctaagcgtgg
    40861 ctcaggcgta catctcagac gtgaccgacg aaaaaagccg ctcaaaagga ctcggcatgg
    40921 tcggtgctgc cttcgggctt gggttcatta tcggcccggt cactggcggg ttgctcagcc
    40981 aatggggata tgccgtgcca gcgtttgtcg cagcaggcat ctcgttcctc aaccttattc
    41041 tgatctacgc ctggctgccc gaatcgctta ccgaagaaaa acgcaaccag atgaccgaaa
    41101 aacgcccagc cgtcaccctg caggcattga tcgttgcatt caaacgcccg tttactggct
    41161 ccctgctcat cacccgcttc ttttacgggc tggccttttc catcttccaa accatcttct
    41221 cgctgtatgc gctggcaaaa tttgatctca ccgcccgcga tacaggcttc gtgttgacct
    41281 acgtcggcgt cctggcggtc atcgtacagg gctttctcgt tgggcgtctg accacccagt
    41341 tccgtgaaga cctgctcatt accgtttctg tggtgttgat gggcatttct ctgctgggtt
    41401 gggcattggc tccttctgtg ttgtggctgt acatcatcat gacccccacc gccatttctg
    41461 gcggcctgct caacaccctg ctctccagca cactgaccaa agccgtggcc ccacaggaga
    41521 tcggcggcat ccttggcctt tccgccgcag tggaaagcgc cacccgtatt atcgcccccc
    41581 tgctgggcgg cgtgctcctg caacaggtcg gcacctgggc accgggcgca ttcggcgccg
    41641 ttgtgatgac gggcgtcagc ctgtatgtat tcaccaccat ttacaaccac cccattgttg
    41701 ccacactgaa acaaggaaag aacgcccccg tccccgcatc ggattaacca atccacaggc
    41761 aatggcatgt caggagttga acatgaccga taaaaccacc cgttacgaac tgccaccctc
    41821 aggcatcacc ctgcttggat atgtggtccg tcgtatgcac aaaacccgct ggctgctgcc
    41881 agtgcccgat ttgcttgtgc agggaaaaac cgacatggcg ttattcaacg ttgccatgta
    41941 tgccgttgcc tcttccacat ctttcggacg ggcatattat cagccgcaat tcaactccgc
    42001 aggcgcagag atcaccgaaa cagacccgct cacaggcgca aacgtcaccg caaaattggt
    42061 tgccacctcg ccgcattatt tcatcaccta caccgccgcc atgccaaacg gtgatctctt
    42121 cactggcagc gaagagatca caggcaccac cgtcggcctg cgcggattgg gcatgcctgc
    42181 cccatccaaa ttcaccttca catcgggcgg gtacaccgcc gaactctcag gcgttctcac
    42241 cagcgagctt gcgctttcac tcattggccg cacccgcatc cgcgcatacg gcttcctcaa
    42301 cttcaaagac aacgcaggca acacaggcaa actcaacctg gatcgcagca gaaaaatcaa
    42361 aattgatatc aacgacaaag ccatggagcc caaattcata tgacttcgat ctggtggatt
    42421 cgccgcgatt tgcgtctgac tgacaacctc gcgcttcatt ctgcgctgaa aacgggtgac
    42481 gtcatcccgg tcttcatcat cgacccggca ttttcctctc aatcgccgcg ccgacgaagt
    42541 ttcctctacg aagggctgca caccctggat caggacctaa aaaaacgcgg ctcgtacctt
    42601 gtcctccgca caggcaggcc agtggacgtg ctgcatcaac tcatgcagga aacaaaagcg
    42661 caggctgttt tcgccgagga agatttcaca ccctatgccc ataaacgtga cacccatgtg
    42721 gctcaatctg tgccgctgca cctgcttccc gggcaaaccg ctcaacaccc cagccgtgtg
    42781 cataaacagg atggcaaacc atacacggtg tacacaccat attccaaaac atggaaggcc
    42841 atgttggagg aaatcgaact cgttcccgcg ccagatgtca tccccacacc ggcagggatc
    42901 tccacagagc cgatccctgc ttttggcgtg aatccgcttt ttccagcagg tgagcaggaa
    42961 gcactgaccc gcctcgatga atttgctttc aaacgcatcc acgcctacga tgaagcccgc
    43021 aaccgcatgg acctggacgg aacatcatcg ctttcaccgt atttgcggtt tggaatgctt
    43081 gggctaagac aagccgtcag gttggcgaag caggcttcgg cggagtcacg aggcgctgaa
    43141 atctggttga acgaattgat ctggcgcgag ttctacatcc agattctgta tcattttcca
    43201 tacgtcagtc atcgtgaatt caaccccgcg ttgatgggga tcaggtggcg aaacaacatc
    43261 gaagaatttg aatgttggaa ggaaggcagg acaggcgtac ccattgtgga tgcggccatg
    43321 cgccaactga aagaaacagg ctggatgcac aaccgcgcgc gcatgatcgt ggcttcgttt
    43381 ttggtgaaag acctgctgat cgactggcgc tggggcgagg catggttcat ggaatgtctg
    43441 ctcgacggcg accccgctgc aaacaatggc ggctggcaat ggacggctgg cacaggcacc
    43501 gatgccgctc catatttcag aatcttcaac cctgttctgc aaagtgcaaa attcgacccg
    43561 ctgggcgaat atattcgcaa atgggtgcat gagctgcgag gattgaacga aaaggaaatc
    43621 catgcgccat gggaacacgg tgtcaaaatc agcggctacc ctgaaaaacc gatcgtggac
    43681 cgtgccctcg tcaaagaaag aaccctgcaa gcgtacaaac aggtaaaaag cgaaagatga
    43741 agactcccct tcatgcatcc gcaccttcaa cttgcaacct gaaaccttca actacatgaa
    43801 ttcaaaaatc acccccgaac tcggacgcgc agccatgcgc gcacttttcg aaacaagatc
    43861 gccgctcggc ccgctgaaag tgatggcaca gcatgtcggc cgcttttttc aaattccact
    43921 gcctggtttt cgaccctatg tggtctttgg cccggaggcg gcccgtaaag tgctgatcac
    43981 cgaacgcgac aaggtgctct ggcgcaacac cgaccccgta accgacctgc ttgggcgtgg
    44041 ggttcttatc accgacggta aagaacatga ccactaccgc gagctgatgg agccgcctct
    44101 acacccgtcg aatctttctg gctatacgca aatgatgctc gaaaaaactg accaagtcac
    44161 ctcacactgg aaaagcggcg acacagtgga catgcttgtt gaaagtcgca agatcgcctt
    44221 gctgatcatc atgcaaaccc tattcagcaa ggatgtgtgg gccgacctgc cccgcatctg
    44281 gaagccgatc ctcaaagcca tagacttcat ctcgcccggg gcatggatca tctggcggaa
    44341 tatgccgcga tttgggtatc gcaagccgct gcgcgaattg gatgattact tgtttgagat
    44401 cattgaagaa aggcgaaaga cgaaacaagc gccccatgac ctgctccagc acttaatcga
    44461 cgcaggcctg actgacaaag tcatccgtga ccagatgctg acaatgatga tcgccgggca
    44521 cgatacatcc accgctttgc tggcatggac cttcgcgcta ctgggtcagc accccgaaac
    44581 gctcaaacat gttcttgcgg aggtccattc aaaagacagg tcgccgctgc tcgaccatgt
    44641 catcaaggaa agcttacggc tgtacccacc cattcacatc ggcaaccgcc gcgtggcaaa
    44701 aaccatggaa ttcgcagaag gcacagtccc tgctggcgaa aggatgtttt actccatcta
    44761 cctgacccat cgcgacccat ccatctggga aaacgccgaa gaattccaac ccgaacgctt
    44821 cgcccacgga agaaaaacac caccaatgag ttacatcccc ttcggcggtg gtccacgggc
    44881 ctgcatcggt gcagctttcg gccaggccga agcacggatc gtactgacca gactgttgca
    44941 gaccttttca tttgaaccga tgaacccaga taagatccac gcgcacatgg gagccactct
    45001 tgagccacgc ccaggcgtat tgatgaaagt acaaccccta attccatgac ctacttcggt
    45061 ttccttttac gcttcctcgt catccccatc ctcatcttcc tcgcaatcac atggtgggac
    45121 aacaagaata accgccccac actggggttc accaatggcc gcgcagtgtg gatcgcgatc
    45181 cttgttcaca tccttctggc ggtaacctac accacgccgt gggataacta cctcgttgcc
    45241 actggggtat ggttttacaa ccccaacctc gtcacaggca tcgtgtttgg ttacgttccc
    45301 atcgaggaat atactttttt tgtgcttgaa accatcctct ccgggctgtg gtggtggttc
    45361 ctcgcccgca gaatttcgga gccaggtggc ttcaaggcat ccatgccagc gcgggttact
    45421 tcgtccattg ttttgttcat tgcatgggtg atctttacag cgatcttttt cagcaacaac
    45481 gagccgctca cctatctttc cattaccctc ttttgggcat tccctgccat ccttccgcaa
    45541 ttgctctacg gcgcagatat tttgtggcac caccgcacgc tggttttgct cggcatcctt
    45601 atccccggta cctatctttc gctgatggat atcatcgcgc tcaacgaaac cacctggtcc
    45661 atttcaaagg cgcaaaccac agggatctta tttttcggca tcctgcccct tgaagaggtg
    45721 gttttctttt tcatcaccaa catcctcatc accttcggta tgacactttt gctgtccaac
    45781 attggcaggc aaaggttcag cgattggaaa ctcaacaact acaaaggcct cccatgaaac
    45841 tcgaacaatt caaaaaacaa caatttctca acatcgaatc cttccgcaaa aacggacagg
    45901 ccgtcaaaac cccggtctgg ttcgtgcagg atggagaaac gcttcttgtc tggacccagg
    45961 ctggatctgg caaagccaaa cggattcgca gggatggaaa gatccgcgtt gcgccatgca
    46021 cagggtcagg tgaacttctc ggtgaatggg cccctgcata cgccaaaacg aacgaatccg
    46081 ttgaaacagt caagcacacg accactttga tgaagaaaaa atacggtctg ttcttcacca
    46141 tcttcggctt catcggcaga atgcgcggcg gttcaaacta caccacgatc agtattcaac
    46201 tgcaaaacca gtgagacagt cacaggtcat gcaccaacaa aaagaggcag ctgcgaggct
    46261 gcctcttttt actttttcgt acgctctact ttcctctacc ccatgttctt cttcattacc
    46321 cgtttcaatg cgaccctgta aatctcggtc agtttttcgc ccatggcttt ggcttcaggg
    46381 ttatccgcac cttgcgagtc catctgggca accagtccgc tcaaggcatc gatgaactgc
    46441 tgattaacca tctcttcgtt ctgcgagagc atcgcatcca acgtggcttc atcaggagcc
    46501 tggagcaaat gctcaatgaa cgcaacctct ggcggagtgc tggcggcctg caaaacctgc
    46561 accatctttt gtaatttacc catgcgggca tagtcattct tttcagaagc ctgcctcaac
    46621 aactgattga ccacctccac tgcgtcctgg gtgaagttct caaggttttc ctgtgtggcc
    46681 ctttcaatat cttcctgggc cagcaaattc tctacaaaat cctgggcctg tttgtaacgg
    46741 gcctccattt gcttgtccac atcgctgacg aagcccagca ctttatcgcg aatgccttcc
    46801 atacgggact tttcttcacc agccgctttt tcgattttct cggtgagcaa ctgaaagaag
    46861 acataatcca tgccgccgcg agccagcgaa acataggcgc ggatacggga atcattttgc
    46921 gattcgagga caaaatccag cagcttttcg cgggtcaaac cctgacctgc atcctgcaat
    46981 gtcttttggg cagcttccaa ctctgcaaca gaatcacgca actgacggcc aaaggcggtt
    47041 tcatccaaca actgacgctg caggtcgatc aatgcgcgtg caatggtttc ctgtccgctt
    47101 tgcatggcgt tttgggcgat gcggctgaaa agcgagaaga attgttcgtc gatcgcgttt
    47161 tcgttctggc ggatcatctc aaggcgggca tctgccgaag tcatgcgaat caacttttcg
    47221 atcagcatga cccgttcctg ctgggtcttg atcatctccg atgaaattcc atccttgttg
    47281 aggatggttt cgatcatcga ttcataggtc aggttggctt gcggtttgag caggtatgcc
    47341 ttgcgctttt caagcggcaa acgctctgtc acctgcttga tcaatggacc gatcaatttc
    47401 tcctgctcgt tgacaggtaa ccccaactca aatgggaaaa aagtgagcag gagttccttg
    47461 tcattgtcgt gatagacaac gggtgtggca aggcggcctt catatccgca atactgacag
    47521 cgggcatggt tcgactgtcc gctcagcagg cgctgtttgg cggcgggttc gtgcgtcaca
    47581 tcgaacaact gctcgacatt ggcgggaatc atctgtttac aacgaggaca agcaatggtg
    47641 gtttgaggca tttacaattt ccgatttacg attttggatt tacgatcaac attcgatcgt
    47701 tcgatattcg gaaaaagcag gccgaacatc gaacgatcca ttttttgaag ttctttatcc
    47761 cagcagttct tccaaacgat tgatgtagcc ttccatcacg gcgaaggtct cttccacagg
    47821 tttgggggtg gtcaggtcta cgcctgcatt cttcaagaca ttaagcgagt aatctgaatt
    47881 gccagccttg aggaagccga ggtagtcttc cacggcgttg ggttccttgc gcaaaatgcg
    47941 gcctgccaag gcatgagcac ctgaaatccc tgtagcgtag gcatacacgt aataatctgc
    48001 aaagagatgt gggaaggtgg cccaggtcat gccgacgcgt tcgcggtcca tgtcaaagtg
    48061 cggaccaaac ccctcggcga acagatcagc catcagttcg atcatggaat cggcggtcaa
    48121 cggttcaccg cgttcggcgc gctggtgcgt ctccagttcg aaacgggcca gagtcggcat
    48181 ttggaagaaa tagcggaaca tgttgctgcc caccgcttca aggatcaacg cgacctggaa
    48241 gtttttgtct ttcacggtgt tgagcaaatg tccgcgcagc aaagcctgat tgaaattgga
    48301 ggctacttca gccacgaaca gcgaataatc ggtgttgatg aactgctggt tcttgaaggt
    48361 gtaataggta tgcatcgagt gaccgagttc atgcgcaagc gtgctcatgc tgaccatttc
    48421 atcggtgtaa ctcatcatga taaagggatg cgtaccatac gaacccgagg aaaatgcgcc
    48481 atgccccttg ccctgattcg ggtacacatc cacccagcgt tcggtggtgc aggcgcgccg
    48541 tacctgagcg gtgtattcct tgccgagcgg ggcaaggcct tcacagatca tatccacggc
    48601 tttgtcgtag ggaatcttca ccttcttctt ttcgatgggt gcccagatat cgtaataagc
    48661 gaatttcttc aaacccagcg ccttgcggcg gatattgaaa taacgatgcc agacagggat
    48721 gtttttcttg aaggtgtcga taaggttgtg gaagacatcc gtgctgatgt tgagatcgaa
    48781 cagcgacgct gaaagcgtgg attcgtgttt tcgcacacgc atattgaaca cgttggattg
    48841 aatattatgc gcgaggttgc tggccagcgt gtttttgtgc gccacatatt gatccatgta
    48901 ggtaatgtaa gcagccttac gcgctttgcg gtccacaagc cccatcaact gcgtatcgta
    48961 attcccctgc atcacatcga aggatttgcc tgcgctatcc ttgatggatt tgaatttgaa
    49021 atcggcattg gtcaacatgc tggtggagtt ataaaaacca ccaaggggct cgcttaccat
    49081 gccaagcact tcttccactt ctgccgagcg cacgtgtgcc tgcttgcgga aaaggttttc
    49141 gacatagaac ttgtaaacgc caagtttttc ttcctgctga aagaattggt caagtttgtc
    49201 tttaccgatc tcaagcaact caggctgaag gaacgaaact gccgaggcta cctgtccgta
    49261 cataccgccc gccttggcac gcatccctgc cgcggtttga tcggtggtgt tcaccgaata
    49321 ggaaaagctc gcatacatgt aagccttttg agcgcggatc aggatcgcct cgaaagcctg
    49381 aaaacctgca agcaaagcgg cggcactttc gcccaaaccg ccttgatgtt tcttcacctc
    49441 ggggatatct gcaataatct gctcaacttc tttttcccag gctttatcac ttgggaaaac
    49501 actttcagaa ttccatttat actttttatc gattttactg cgtggaggga tggaagtagc
    49561 catggggtat ccttttcatc aatcaattat ttttcgattt taccattgca agcacaacac
    49621 ttaatttcat tatcgaggca gggagaaaca aatcctcgca ccagattcag attttgccga
    49681 gcccgtcaca gcatccgccc aaatatgccc accatgcgct tccacgatgg cacgcgcaag
    49741 gtaaagccca aggcccgccc cctttgtttg ttttacggca tttgtcgaac gataaaaacg
    49801 gtcaaagatg tgaggcaggt ctttggcttc aatccctggg ccttcatcgc ttacacaaac
    49861 gatcacctgc tcgggcaata cactcccgct gatcttgatc tcacccttcg gcgcatattt
    49921 caacgaattg gaaaccaggt ttgagatcac ctgctccaaa cgattttcat cccccaaaat
    49981 aacggggaaa ttcgacggga aatcggtgac aatgctatgc ttcggagatt gcgacgcaaa
    50041 ccgctcgctc acccttaacg caaggctggg tacggcgaca tcagcctgat tcaagctgag
    50101 accacccgcc tgcagccgtg aagcatccag caagtcatcc accatcttcg acaaacggtc
    50161 cgcctcttct tcgataacgg ccagcgaatc attgatggtg tgtttatccc agcgtgcatc
    50221 atcacgccgc aacgtggagg cgtaaccctt gatcaaagct accggggtac gcaactcatg
    50281 gctgacaatg gaaataaacg tcgctttgat ctcatccgct gtgcggaaat gggtgatgtc
    50341 acgcacgcta agaatgacgt tacggagctt gccttcttca gacagcaagg gagcgtaggt
    50401 aattcccacc ggaagcggcg gtggcgcagg tttcgcaagg tcaccttcta cgtacagggt
    50461 tgcgttcggt gtgagcggcc agccctccgc caccgaatct tcaagggtaa tgccttgcgg
    50521 tgtctttttc cacttgatga tatctgcatg gtttttccca gtgatctcct cacgaggttg
    50581 accaaataat ttttcaaaag cgccgttaca acgttcgatg tcgaggtttg catccaaaat
    50641 caaaatgccg tctgccgctg attcgagtaa tgcatccagc cttttctttt cataagaaac
    50701 ctgaccgtac aactgcgcgt tatacacggc gatggcagcc tgatcggcaa aggattgcaa
    50761 gagaacgcgg tcatcggggg taaacaagtc ggcgtaattg cggaagataa agatcacacc
    50821 aatgacctgt ccatgtgcag caaggggcag accagtgccg tttaataacc ccatgctggc
    50881 agtataggtc agatttttca acatgcggtt cagttcacgc acgtccaatt cgcgaacttt
    50941 ttcttcggca agcaacgggg ttaagtagga gagaaatgcc ggggcaatgc catgtgccgc
    51001 gctgacgcgc cagccgtcgg cttgtttgag agcgatcagt ccagcctgcc cggccaacat
    51061 ctcaatcgaa acgcgcaaaa ttcgcgccag caacttttca aggtctagtt cttcagtgat
    51121 caggcgcgaa atttccagca aataatcgcg ttgacggacg cgaaaatcgg gaagcatcga
    51181 aggaataatg gacattgctg ttattttatc actgcgctac ggcaaaaggc aaaaacagcc
    51241 aaacccacct ctggataaca tatttgtttt gcttgcatta atgaagggaa ataacattgc
    51301 aagtaagtta acatggttgt aaaatgatga gaaattaata gtacttaagg ctaatttcag
    51361 atatccttca tgtaaattgt tgcaattttc agccttcatt ctgattttgc aggagcaaat
    51421 gatgagaatt ttttcaatcc aagcgttcaa tcaatccaaa tctgccatgg ttcgcctgtt
    51481 cgtttttgtt ttgcttttgg cgtccatgct ggcacaggag catgtgccgg gggtgcaggc
    51541 tcaggcgggg acaccccaac tcattttgac caaaaccatt gagggtggcg caaccacggc
    51601 cagccttggc gatacgatcc ggtatcgcat ccgttttgag tgcagcagtc tgacaacctc
    51661 ctgcggacaa atggaaatta ccgatgtgct ccctgccggg ctgacgtatg ccccaccccc
    51721 ggcttcttca gcaccgaccg gtttcaccat tacagaagcc ccggctggaa cgattaccat
    51781 tacaaaagat gacaacaacc tgctcgatgg tacccagtac gatgtggtca tcgccgcaac
    51841 gattgattat gatcttcgtc ctcttcctgc caccatcaac aacacagtca atggacgggt
    51901 ggatccgccc ggcgccgtca gctggcaaaa cgcaactcct gcaagcgcac ctcccatcac
    51961 tgtgagcaat gtcggtgaat attgggatct gacaaagacc ttgtatagtc cgtccgtcaa
    52021 ccccaccatc aatacagatg ttacctatcg catccaactc tgtccaacca caccgccgcc
    52081 cggcaccagc aacgtggcgc ttgaaaacat tacgatcaca gatacccttc ccacaggagc
    52141 aacgtttgtc tctgccagcg atgcaggcac agaggcagcc ggtgttgtaa cctggccgct
    52201 cattgcgggg cctgtcttcc cacccacctg tgttacccgt tatgtcacaa ttcgatataa
    52261 cagcccaacc tttgcagtag gcaacaatgt tacgaacaca gccaacgcca gcggcacata
    52321 cacggattca ggcggtggta cgataggacc aatcagcatc gccactgacc caatcaccca
    52381 tccgattgcc accgtgctgg aagttcccac ttacagcaag aatgatcaag gggatccagt
    52441 gggtatcact ggaactgccc ggtttgtgtt gacgctcaac acaaacggca cgaactcgcc
    52501 agccaatgat ctgattctca ttgatacact gcctcctgaa ttacaggtta cttcagtgac
    52561 cagcggtcag tggaatgctg catttaatta tgttcgggct tatgtggaat actccaccaa
    52621 ctatggcagc agttatacag cttttccggg ccagcccatc agctacaaca gtaatgccac
    52681 atacactgcg cccgctgcca acataaccaa cgttcgctgg cgtttcgaat acgatcccga
    52741 tgcggcggca cctttcgcat ttacacaggc tgggcttccc ttcgcttggg cgtttaccac
    52801 cagcccgcaa atccgtgtaa ccccgcgtgc aacggcaaca accgcagatg ctccttcggg
    52861 cgtagccatg cctgcagctg ttgcagggac acgctatgaa aactgtctgc aaaccagccg
    52921 taccagcaat gcaggtgccc ccgtaactga cccatgcgat attgaaggga tgacagttca
    52981 aggcacattg gccagtttgc gtgcatcgaa ggctgagacc cgcggcacca gttgggatgc
    53041 tgataacgat ccgctcatta atacatttac cagtgacagc actttattgc caggtgatac
    53101 cctgcgctat accatcacgg tcagtgttac tgagcgttca tctcaggcga tgatcgaccc
    53161 aaccattctt gatacgattc ccgctgactt gatcttcgtc cgcaatggaa cggcatcagt
    53221 caacggtacc ggttgtccag cacttgccag tcagccgact ttcacccaag caggcacaca
    53281 actgaaatgg gatttcacag gtttctcgct cgaacatcaa cccagcggac ttggcagctg
    53341 caacctggtc gttgagttct atgcccgcat ccccgccgga caaaccccag gtacccgcac
    53401 caatgacctg atccttgtca ccgactcgga tgacatcgta tgcgaaaacg gcacagagat
    53461 cgttgacact acagatatcg atagcgatgc caacaacacc gagcccgcct gtcaaaatcc
    53521 agatacttat gtggtggagc gttcagctgc attacgcggt gaaaaatgga ttcgtagtgt
    53581 cgatcctgaa aacagtgtag tggtcgactc taccaccttc caacccagtg cctcttgccc
    53641 ggatggcggc actgtaggtt tggctggagg cggcagcaac cccttcacca gattcccctg
    53701 tatctcacag gcattccctg aaggagcttt aagcatcggt caacgcgttc caccgccgct
    53761 taactctgcc ctggatgatt tcgaatacaa cttgcgcatc tacaacgatg gcaatgtcga
    53821 aatgttaagt tacgccctgt atgatatttt cccctattat ggcgacaccg gctccggcgg
    53881 tactttggcc aattccccac gcctctcaga attccgcccg atcctgcgtg gacctgtgga
    53941 atttatcagc ggtccaagcc taagcagcac cgacttcacc atcgaatata acaacaccac
    54001 caatccctgc cgcccggaag tgttcaatca gcctgcaggc actcctaata tcccagccgg
    54061 gtgtaacaac acctggagtt ccacctggtc ggcagatgca cgctcattcc gcattttgct
    54121 gaattccccg gccgtcatcc cgccatctgc caccacctcc gaagtacgct tcggcattcc
    54181 catgtacata cccaaggatg cgcccacagt cggcgtattt gacaacaacg acccgttgtc
    54241 acttgaaatt gcatggaatt cattctcaca cgtcggttct tacgacagcg accccaatgt
    54301 cccagtcgtc attcaagact tgctggcatc tgaaccgcgc aaggtcggta tcaccattcc
    54361 cgaacgcatg agcgttggta accgcgtctg gcgtgactcg gataacagcg gtcacatcga
    54421 tgccccagat gatatcgccc ccggtattgc aggggtaacg gttaacctat accgtgatgt
    54481 ggatgatgat ggcgtacccg acgggccagc cattgccacc accactacgg attctctcgg
    54541 ctactatctg ttcagcaata ttcctcatga tctggcagat gttaataaca accgctacct
    54601 tattggcatt ccatcaagca attttggatc cgggcagcca ttgttcaact tgcgcagcag
    54661 cacaggtgtg ttcgctacaa cagatgacag tgatgacaat ggcattgacc ccgtcactgc
    54721 cgggcaggaa gttttcagcg ccagtttcat cctgcaacct accaccgaag tcaccggaga
    54781 aaccgatctt tcatccaacg acctcgatgg tccccccgga gaacgccgtg gcaccaatgc
    54841 agaacgtgac tacaacagcg accttgccat tgactttggt ttcttcggcg gtactgatgt
    54901 acctttcagc atcggcaatc atgtctggtt cgataatggg cgcactggcg tatccacttt
    54961 caactatgcc ttgaacaatg acggcatccg ccaggcaaca gaactgccgg tcgtcgggtt
    55021 ggatattcgc ctatatcgtg acggcaatgg caataacact cccgaacctg cggaaatgat
    55081 tcgcaccgat ataaccgacg ccaacggctt ttaccttttc gataacctcg accctggacc
    55141 ctacattgtg gaaattccag ccagtgaatt cggtgctggt cagcctctcg aaggctggaa
    55201 caacagtcag gtaataggaa ccgagacaac cggtgtgccg ggcaatacca atattccaaa
    55261 cgtggatagc gatgataatg gtgtcgattc cagcactccg catgttgatg gaatcttcag
    55321 tggtgtcatc gtcctcacac gcggcaccaa cgaaacgact ggtgaaactc atatcagcaa
    55381 tcagccagac ccggatggca atgccgccac gcccaacctt ggcatcaacc ccaccgaatg
    55441 ggatggacct gcttcacgtg gacgttacgg cgaaagcgac acaaccagca acctaaccat
    55501 tgactttggc ttcttcgaag tatattcact cggtaatgta gtttgggtgg atgatggaac
    55561 gggcggtggc actgcagatg atggcctgcg caatggtacc gaacccggcc tgaatggcgt
    55621 caccgtcaac ttatatctgg atgcgaacaa cgacggacgc ccagacacag ttacgcccaa
    55681 ggcaaccact accaccgcca atggcggata ttaccgcttc gacaaccttg cggctgatac
    55741 ctatctcgtt gaaatcgtcc tacccgctgg atattcgagc agcccaatcg atgcaggcga
    55801 ccccgatacc gacgaagatg attttgacga caatggtgtg gttccggcca cgatctcaac
    55861 tgttctcagc aacccggtca ccctcgggcg tggaacagat gaacccacgg ttgatagtga
    55921 cgcggccaca gccaataatc ctgaacctgg tgaagcaccc aaccaccact cgaatcgcac
    55981 cgtagacttt gccctaactc ttggctacag cctcggtaac cgtgtctggg tggatttgga
    56041 tgacagcggc acgatcaacg ccgccgatgg cgtcacaccc ggtcttgata acatgaccgt
    56101 gaacctgtat cgctccagcg accccagcac cattatcgcc accaccctaa cggctggcgg
    56161 tggatattat cgcttcgata atctgctggc aggcgattat attgtggaag tggtctctcc
    56221 tgttggttat accagcagca gtgtagatgc caacgatccg aacaccgata atgatagcga
    56281 tgacaacggt gtgattattt ccgcaaccac ggtccgcagc aaccccgttt cactgggcgg
    56341 catagaacct tccactgatg acgacccact gcccaaccca gccgccacag gtgaagcccc
    56401 gaatgcccgt tccaaccgca ccgttgactt tggcttcttc tttgtgcctt attccctcgg
    56461 caaccgtgtc tgggatgata gcgacggtgc aggcggcaca cctgacaatg gtgttcgcga
    56521 tggcgctgaa cccggcattg atggcgtcac agtcaacctg tatcgcgact ctgacaataa
    56581 caacacccca gatggggcaa ttattgccac agatacaacc agcggcggtg gtttctatcg
    56641 ctttgacaac ctggctgcgg acacctatat tgtggaagtg attccgcccg ccggttatgc
    56701 cagcacactt gatgcaggcg acccggacac agacctcgat gacgatgatg acaacggtgt
    56761 cgtatttgca ggtgcgaaca tccgcagcaa ccctgtacgg cttggcccgc ccgaacccag
    56821 cgaaccgact ctcgaaaaca acccggcggt caacccagaa accgccgatg gcgaagctgc
    56881 agacgatcac tccaaccgaa ctgtagactt tggctttgtg ttgggctaca gcctaggcaa
    56941 ccgtgtatgg gatgatagca acggcgcagg cggtacacct gagaacggcc tccgtgatgg
    57001 tacagaaccc ggtatggatg gcgtcactgt caatctttac cgtgactcga acgacgacag
    57061 caaccccgat ggcggcatta tcatgtcaac ctccacctcg ggaggaggct tctatcgttt
    57121 cgataacctg atggctgata cctatatcgt tgaagttgtc atccccacag gctataccag
    57181 cacgcttgat gcaggtgacc cggatacgga catagatgac gacgataatg gtgtggtcat
    57241 cgttggttcc aatgtccgcg ccaacccggt cacgctgggt cctgccgctg ttccggagcc
    57301 tactctcgaa aataacccgg ctaccaaccc agtagctggt gaatcaccgg atgcgcgttc
    57361 caaccgcacc gttgacttcg gcttcatccg ctccagcact cccccgccgc caaccggttc
    57421 agggattcag atcgtcaaga ccgccagcgc gagtgttgtg aagccgggtg atactgttgt
    57481 gtattcctat gttgtttcca atactggcac caccccgctt agaaatgtca tcgttaccga
    57541 cgacaaatgc tcccccgtga cctacataag cggagatgtc aacgcgaaca acatcctcga
    57601 aacgaccgaa aagtggaatt acacctgctc cgctattttg agcgaaacga cgaccaacat
    57661 cgccacggta accggtaaag acccaacggg cggcgatgtc aaagacacag acacagtcac
    57721 cgttacggtc gacacgaatg gtttctccaa gatcatcacc ggcaccagcg aaaccttcac
    57781 caacggcatg gatgtcgcca ttggcgagat cgtcacctac aagatcacca tgagcctgcc
    57841 cgccggactg ccctacaaca atgtggttgt caccgaccgc atggacaagg gtcttgcttt
    57901 tgtagactgc gtatcggcca ccctgggcgg cgtggatatt ctcaaccaga tctgcccgcc
    57961 gttggtctct gcgatcaaag acccaattga cccagccagc aacccagcca acccgggccg
    58021 tcaggcacaa ttcaatatcg gcaatgtatc tgcttccaca aagccgacgg cattggtcat
    58081 cacataccgt gccatcgtgc ttgacgtcat cgaaaaccag gaaaaggata ccttgaacaa
    58141 caaggccacc ctcacatggg atggaggctc cctcactgcc agcgcgcctg aggttgtcat
    58201 cctcgaacct gaccttgata ttgagaaagc tgccaaccca accacggtca cagcaggctc
    58261 atccgtcgag ttcaccatct tcatcacgca cacttccttg agcctcacgg atgcattcga
    58321 tgtggtggta accgacgttc tgccgaaagg ccttgaatac gtggcatgta cccccatcac
    58381 ctatgagggc gtcgttccaa ccaaacaacc tgacccatgc agcatcccca atggagacac
    58441 cctgcaattt gaatgggatg tgctcccgct cggtggaaac tccgtcatca agttctttgc
    58501 ccgttatgat ggctcgcaag tcagtgtcat gaacagcgcc gcagttgcat ggacctccct
    58561 gcccattgat tacatcaacg gcaagccagt ccaacactct gggtacaacg acacatctac
    58621 tgaacgctgg tacgacccga acgacctggt caacatctac cagatcagcg acaacgtctg
    58681 gatcaacccg ccgaccatta ccgaaccaat cgaggaagag gaactaccgg gcagccttcc
    58741 tgctacaggc ttcgccccga acgtcatcac cgccctgccg cagcaacctg ccaacaaggc
    58801 ctacacaacc accgatatct gggtggagat tccaagcatt ggcgtaaaca tgccgatcgt
    58861 aggcgttccg ctcgttgacg gtgattggga cctctcctgg ctgtggaatg aagcaggctg
    58921 gctcaatggc acggccttcc ccggatggga tggcaacagc gccctcaccg gtcacatcac
    58981 cctgcccaac ggcagacctg ggccatttgc catgctgggc aacctcaaat ggggcgataa
    59041 gatcatcgtc catgcgtatg gcacggccta cacctacgaa gtccgcgaga ctcgtacggt
    59101 cagccccgac aatatgacaa tcctcaaaca cgaagaggaa tcctggctga cgttgatcac
    59161 ctgtaagact tacgacaaag ccaccaacac ctatgcccgc cgtactgcag tccgcgctgt
    59221 gctcattagc accaccgccg acaagcaaac caaggaagcc aaaagcggac gctagaaact
    59281 aacccaaaca aaaacttccg ggccaacacc cggaagtttt tgttttaagc actttccact
    59341 tactttctac ttcatttcct tctcagccac cgtcaatgca tcttcaaata tcttcaaacc
    59401 ttcttccatt tcagatttac tgatggaaag cggaggcgca atgcgaatga cgctcttgcc
    59461 gcaggaaagc atcaacaagc cgcgttcgaa accgagatca acaacacgct ctgtcaaatc
    59521 atggtcgggt tcttttgttt cgcggtcctt cacaaactcc acaccgatca tcaagccttt
    59581 tccacgcaca tcaccaatac tggggtgacg tgatttaatc tcttccaggg catccaacgc
    59641 atagctccct acctcttcgg cattcttcat gaatccattt tcgatcaggt caatcgttgc
    59701 aagagatgcg gcacaagaaa tggggttgcc gccatacgta tttccatgtg tgccaatttc
    59761 ccaatccatc acagacttgc gggcgataca agcgcccaaa ggcataccag aagcaatgcc
    59821 tttggcagaa gtgatcatat cgggctccac gccaaaatgc tcaatagccc accacttgcc
    59881 tgtgcgcccc ataccagact gcacttcgtc aaggatcatc agaatgccat gcttgtcaca
    59941 cagcttcctc aaagcggggt aaaaaccttc gggcggaatg atgtagccgc cctcgccttg
    60001 aatggtttcc accaaaattc cagccacatc cttgggcgga agaatttggg caaggatctc
    60061 ctcttcaata tagcggacga cagcttcgcc ataatcttcg cctttacggc gttccaacac
    60121 cgggcgatac gggtttggaa acggcgcatg ggtgacgcca ttcatcaacg gataaaaccc
    60181 accatggtat ttcgctttgc tggcggtaaa agtcaccgct cccatggtac ggccgtggaa
    60241 cgcgccggta aaaccgatga aattcgtacg cttggtatgg taacgcgcca atttgatcgc
    60301 tgcctctacc gcctcagtgc ctgagttggt catgaaggtc agcgcatctt ccttgaacgg
    60361 tgcaatatcg ctcaattttt caccaagccg aatccaattt tcgtgataaa aatccgatga
    60421 aatatgaatg aatttctcag cctgctcttg aatggccttc actactttgg ggtgtgagta
    60481 tcctgttgaa accacggcaa taccgcccat aaaatccaaa aagcgattgc catccacatc
    60541 ccatacttct gttccttttc catgatccat tacaaatgga tatccacgcg ggtatgaagg
    60601 tgatattacc tttcgatccc gctcgatcat tgccttggcc tttggcccgg gtaaattagg
    60661 gtttgtcata cattctcctt gtttcaatta agccgctttc aaggcgcaca gattcaggcg
    60721 accccgctgg gtattgaaaa cttgtgcacc tgaaaagcgg tcttatttat gaacttgtaa
    60781 ctcagctaca gccgagtcgt cccccggtaa atccatgcgg attaagaacc gcctcacact
    60841 caccgaaaat tgttcagccg agaaaaaaac atccttacgc ctcagcaact accggcgcag
    60901 ggtgaatcgt tgatttcatc cagattctca ttttttcggg aaaattcttc caataaaagg
    60961 tcaaaaatcg accttccacc aaggaatatg gcaattatac cgtatgccct gtttgccgcc
    61021 ttttccgccg ttgcaacccc atgcatcaaa gatttgactc ctcatttcaa ccctgctatt
    61081 atccttttat gcgaaaaaat ttccttgctg atttttggca ggctctacgc gacattccgc
    61141 gctggctgag gccggggttg ggcataaaac gctggcttac cctggtgctg atcggaatca
    61201 ctctgttggg gttggggctg gcagtcattc ttattgacct gtatcgcaca gactcggaaa
    61261 acccgatcct gctgaccttt ctctcctatg cctcgttgcg cttcattccg cgagtgtggc
    61321 gtgccgtcat ttttggcggg ttgggtgtgg gcgttgtgat ctatggcatc gtgcaactca
    61381 accgttccct gttgacgcca ttcgtccgcc ctggtgcgga tgtgtacggt cagcttaccg
    61441 actttcgccg tcgcgaacgc ggtccacgca ttgtcaccat cggtggcggg catggacttg
    61501 ccacactttt acgtgggcta aagactcaca cccgaaacct gactgcagtt gtgactgttg
    61561 cagacgatgg cgggtcatct ggcaggttgc gcgaaagcca tggcattttg ccgccgggtg
    61621 atattcgaaa ctgtctcgcc gccctttcca atgacgaggc catgttaacc cagctttttc
    61681 aatatcggtt cagcggctcg cccggtttgg aaggtcattc atttgggaac ctcttcatca
    61741 cagccatggc tgatattacc ggtagctttg aggaagcggt ggcagaatcg ggccgtgtac
    61801 tttcggtaaa tggccgcgtt ctgccttcta cattgcacga tgtaaaactg gtcgccagca
    61861 tggaactccc ccactcactc aacgaaatcc gtgttgaggg tgaaagcaaa attccaacca
    61921 tggcaggacg agtccgtcgt gtgtggctcg aaccagataa cgctcctgca ttcccacctg
    61981 ttatcaaatc cattctcaat gccgatgtga tcgtggtggg gccaggcagt ctttttacca
    62041 gcattctccc caacctgctg gttaatgacc tgctagccgc catgcatgcc agccgagcga
    62101 taaaaatata cgtctgcaat atcgccacac aaacaggtga aaccgactca tacacctgct
    62161 ttgatcatgt ccgcgcactc gaagggcatg tgggcgagga tctgttcgat gtcattttgt
    62221 gcaacgataa ctaccgcggc ataatcagcg ctgactcaaa gtgggtattg gccgatgaaa
    62281 agatattatc tgattctcgc acatactgtg ctgatcttgt agatgacgat cacccatggc
    62341 ggcacgactc tgaaaaactt gcccgcgaaa tcatacaaat cttctctgaa aagactgggc
    62401 cattggtcta gtcaagagtg cttttcccaa aaaagtaaac gattacattc gtcctattat
    62461 ttttggacgt acgaaaattc acaaattctg cttaaggcag taaaattaag gctgtatgaa
    62521 ggctcatacc agagcaggca caaattttag gatagatgtg tcctctgttg taacaaaacc
    62581 aaattggagg attttctcat gacagtaaaa attggcatta atggctttgg ccgtatcggt
    62641 cgccaggtac tcaaggcaat ccgcgatatg tatccaaacg aattggaagt cgttgccttt
    62701 aacgacattg gcgataccaa gaccatggct cacctgctca agtacgactc aacctatggc
    62761 cgcttcaacg gttccgttga gattgccgac gatggtctcc tgatcgatgg aaaaaaggtc
    62821 aaggtcttca aagagactga ccccgctgcc atcaaatgga gcgagctcgg catcgacatt
    62881 gtgatcgaat caactggctt gttcaccatc aaggatgacg gtgtcaacaa gaagggtaag
    62941 accgtgaagg gtgccgtcaa tcacatcacc tctggcggcg cgaagaaagt catcatctca
    63001 gcacctgccg aaggtgaaga tttgaccgtt gtattgggcg taaacgaaga caaatatgac
    63061 cccgccaaac accatgtggt ttcgaatgcc tcctgcacca caaactgtct cgcgcccgcc
    63121 gcgaaagttg tgcatgacaa actcaatatc aagcgcgctt tcatgaccac cgtacattcc
    63181 tacaccaacg atcaaaaagt gctcgatatg ccgcactcgg atctgcgccg cgcccgcgcc
    63241 tcaggcttga atatcatccc gaccaccact ggcgccgcca aagctgtcgc tctggtcatc
    63301 cccgacctca agggcaaatt cgatggttac tccctgcgcg tccccacccc caccgtttcg
    63361 gttgtggatt tcaccgcaga ggttgaaaag gaaaccacca ctgaagaact gcgccaaatg
    63421 ttccgcgatg ccgccaaaga accgcgcttc aagggcatcc tcgaagcggt ggacgaaccc
    63481 ctggtcagca tggacttcaa gggcagttcc tacagctcat ccgttgacct gccgttcact
    63541 tccgtcctcg gcaaagaaaa gaacaacttc atcaaggtcg ttacctggta cgataatgaa
    63601 tggggttact cctgccgtac tgctgacctc gcggcgatga tggcaaaaac actgtaaata
    63661 ttttgggtag ggacacggcg atgctgtgtc cctacaaaat taaaatgaac aagaaaaccg
    63721 taaaagatat tgatcttaag aacaaacgcg tactcatgcg cgtggatttc aatgtgccga
    63781 tggcagatgg aaaagtcacc gacgacaagc gcatcaaagc cgcactgccg accattaaat
    63841 atgttttgga acagggtgct tccctgcttt tgatgagtca tttgggacgc ccaaaaggcg
    63901 gtccagaccc tgagtttagc ctgcgtgctg cttcggaagc attatcggca ctgttggaga
    63961 aacccgtgca aatggcgccc gattgcgtcg gcgcggatgt tgaagcaatg gcaaaaggat
    64021 tgaagtcggg cgatgtgctc atgcttgaaa acacccgctt ccacaagggc gaagaaaaga
    64081 acgatctcga ccttgccaag cagatggccg ccctgggtga tgtgtacgtc aacgatgcgt
    64141 tcgggtctgc tcaccgtgcc cactcctcca ccgaagggat tgcccgcttc ctacccgccg
    64201 tatctggttt tttgatggaa caggaacttg aatacctggg ccgcgctgtt gccaaccccg
    64261 aacaccctta catcgccatt ctcggcggtg cgaagatcag cgacaaaatc cttgtggtgg
    64321 aaacccttgc ctcaaaatgt gacaagttga tcatcggcgg cggtatggca aacaccttcc
    64381 tcgctgcgaa gggattaaac atgcaggaca gcctcgttga agcggaatcg ctcgaaaccg
    64441 ccaaatccct gcttggtaaa ttgggcgaaa aactcgtcct gcctgtggat gcggtcatcg
    64501 ctgataaatt cgatgccgaa gccaacactc aggtggtgga tgtggacaag atccccgcag
    64561 gctggcgcat gctggatgta gggccgaaaa ccatcagcct gtatcaggcc acattgaacg
    64621 ggagcaaact catcgtttgg aatggacccg ttggcgtctt cgaaatgccg aaattcgccg
    64681 aaggcacatt cgcccttgcg cgcatgctgg cagaatccaa agccactacg gtcattggcg
    64741 gaggtgactc ggccagtgca gtcaagaagg ctggcgttgc caaacagatg actcatgttt
    64801 ccacaggcgg cggcgcctcg ctggaattcc ttgaaggaaa agagttgccc ggcgtggcgg
    64861 cactgctcga taaataattt tcaaaaggat gggcgaaaac gcccatcctt ttgatttacc
    64921 agaaccttcc acttcttttt taaagcgcaa ataaaccaga atttcacatt tcagtttaca
    64981 aacaatttac aggggtacaa cctaaattaa tgacactcga ctataatcta aaaaaatgca
    65041 atcggagata gtgtatgtcg ttcaacatcg gtgaaaacgt cggcccatat cgaattcttg
    65101 aacaacttgg gcagggtggt atggcaacag tattcaaggc ctatcacgcc tcgcttgatc
    65161 ggtatgtggc tttaaaagtt ttacaccctg cattcaacca ggaacagtct ttcatttcac
    65221 gctttcaacg cgaagcgcgg gtggtggcga aactcgaaca tccaaacatt gtcccgattt
    65281 acgattattc cgaacatgag gcacgcccgt acctcgtgat gaaatacatt gagggggata
    65341 cactgaaggc gcggttgaac aaaggtccgc tttcagcagg tgagattgaa acagtcgtga
    65401 atgcgattgg agcggcgctg gcctacgcac acaggcaagg cattctgcat cgtgatgtca
    65461 aaccatccaa tgtgatgctg gccacagatg gattgatcta tctcgctgat tttggccttg
    65521 ctcgcattgc acaggctggc gaatcgacca tgtctgctga ctcgatcatg gggacgccac
    65581 agtatatttc gcccgaacag gccatgggta aaaaagacct cgattcaggg acggatatct
    65641 actcttttgg tgtgatgctg tatgaaatgg tcgtggggca ggttccattc agtgcagata
    65701 cccccttctc catcattcat gaccatattt acacgcccct gcccctgcca agaaaaataa
    65761 acccaaatgt tcccgaatct gttcaacggg tgcttctaaa ggcattggca aaagatcgca
    65821 ttgaccgcta tgaaaccatt gaagagctgg tacaagcctt caaagacgca tggactgaag
    65881 ccggtgtccc catgcagggc acatccatca ccatgcactc caccaatctt cgcaagcagg
    65941 ttcaatcaac ggcatccaaa cccaagcctg ccatgcccaa cctcgtcaat gaaaaaccaa
    66001 gaccgcaggc caaggcagcc gcaaaaaaag gcagcgccgt gtggatgtgg ctcggcgtag
    66061 ccctgactgg ttttatttgc atcatcatcg ccttcattgc aattcgcaac aacaaccgaa
    66121 ttgcaccctt gcccgttcag cctacacttg ttccaactgc cgtcatttta gagccgtcac
    66181 cggcgatccc aacccaatcc gcgccgccta cattccctcc cttaacaccc ccgctcggca
    66241 cagatacaac tggtcagccg ccgctcaacc cacaaggcag tgacacaccc gaaatcgcag
    66301 ccgcaaggga agttgtggcg aaaaatccgc aggatgcaga tgcacacctt cagcttgcct
    66361 ttgcactttg ggatgccaaa gaaattcgcc cgtccatgga ttcgctgatg caggcaatgg
    66421 gactggctgg ccccggtaac acggattttc tacaaaaggc agccagcggg tttcatcaac
    66481 gcgaggcatg ggtggcagct acagccatct acctgcgttt gcaggcaagt tacgctggcg
    66541 cgcctgttcc tgaagacata aaaatcaaca ccaacgaatc ggcatacaaa gcagcagacc
    66601 gaaaagattt cctgctgctc attgcgttcg accagatcga tgccatcgac ccgcgtcttg
    66661 gacatgttgt ccgtgggcgg caggcattat tcaacaacaa tttgcaggaa gcccagcttc
    66721 aactcgcaga agccgaaaaa ctcgagccca agatccccga aatgttttta ctcaaagctg
    66781 agatcgccct gcgcaatggt aatacctctg aagcaagaac cattttcaac aacacgctca
    66841 acaatccagc cacacccgcc tggatgcgcc tgatgattga agagctgctc aaaaccatgc
    66901 aataaggaca caacaatgcg aaaaccactc gtagctggca attggaaaat gaacaaaacc
    66961 atcacagaaa ctcgtgacct ggtcttcaca atgagcatga aattgcgcga aattgacggc
    67021 gtggaaaaag tcatctgccc gccgttcatg tcactcatgg ctgcagccgc actgctcgaa
    67081 ggctcgggca tcggcctcgg cgcacaaaat atgcactggg aagaaaaagg cgccttcaca
    67141 ggcgaggtct ctcccgccat ggtcaaggag ttgtgcggat acgtcatcct gggccactcg
    67201 gaacggcgct cctattttgg agaaaccgac cagatcgtga atcggaagct gctcgctgcc
    67261 caagccatgg acctggtacc gatcgtttgt gtcggcgaga ctttggaaca gtacgaatca
    67321 aaactcaccc gtgaagttgt ttctactcaa acgagtcaga gtctcaggga tgtatctcca
    67381 tcctatgccc cgcgcatgat cgtagcgtac gaacctgtct gggccattgg cacaggcaaa
    67441 gcctccaacg ggcctgacgc caatgacgtt gtaaaagatg tcatccgccc agcgctggca
    67501 gatttatttg gaaacgaaac cgcacaagct atccgcgttt tgtatggcgg gtcggtcact
    67561 gctgcaaatg ccgccgagtt cttcggccag cctgatattg acggcgctct tgtcggtgga
    67621 gccagcttga agatcgacga attcgtagcc atcgccaaag ccgctgcaag caccgcagca
    67681 taaccctgaa ggaatccaaa aaaattcttt tcaatcatgc ccttcactgg ctttctgctg
    67741 ttcattctgc tgcttctgcc gctggtattt ctccagcggc ttttgcatcg tgaaattcag
    67801 gctgtcctgc tcatcctcac ccgcaactca cagttgacca tcggcttgtt ttcggtgctg
    67861 tttttccccg gtgtgttcct gcatgaatta agtcatttcc tcatggcaaa gttactgcag
    67921 gtgcggacag gaaacatttc actcatccca gccgccctgc caaatggacg ccttcaaatg
    67981 ggttatgtgg aaaccgaaga aaccgacgta gtccgcgatt cattgatcgg gctggccccg
    68041 ctcattgcag gctcgctctt tatcgcctac gcaggcttaa accgtcttgg gtttggcatg
    68101 ctcatggatc tgctggccaa tgggcaattt gccctgtttt ggacagcact gggttacctg
    68161 cctcaagtcg atgattttta cttatggttc tatctcgtat ttgcggtttc cagcatgatg
    68221 ctgccctctg aatcagaccg ccatgcctgg ctcccgttgg gagtatggat agcagggctg
    68281 tttgcattgg cgatctttgc tggagcaggc caatggatgt tggaaaacct ggccccctca
    68341 ttaaacgatt tccttggcac agtagccact ttgtttgggt taagcgttgc tgttcatgtg
    68401 atccttttat tgcccaccat gcttctgcac cgcgcccttt caaggatcac agggttggat
    68461 ataaaaaagt agcacagacg caaattcgat caagaaaaag ggaaccacca cttgggtctg
    68521 ctttctttct gcagatgagc cactgtttcg gggtgcccca tttgaatcag cactttcttt
    68581 gccatgtttt gcacaacttc actatcatca tttagcatgg cagtaatggc atggttgatt
    68641 tggattcggt ttaacatcaa caggtgaacc gcaggcaggg caattgagca ggtcgatttt
    68701 catggagtac accctcctac aacatgctta acgaatgggc ggcatttccg cttttttatt
    68761 gaaagtgttc caagccagcg aatgcagggc ccttttttga gacggaatgg attcggcgcg
    68821 ctttagccat tctgcttcag cttgtgcggc ttcggcaaag caatgtgctg aaaattccgc
    68881 ccgctctttt acagtggcgt tggcaagtct gcgcgattct gagaggaatt tcttttccat
    68941 tgcatcgcgg tcactggcgt aggttttaat ccgttcagaa tagtttagca aggtggcacg
    69001 gtgcaactgt tcatgcttcc aatatgtcga cgctgagtcg tattttgaag caggagcagg
    69061 cccaaggtta agggaagaag cggtgtccat ccacatgggc ttgaaaatgc tggtacaagg
    69121 ggcagatgtg ccggtgacgt aaatgagcgg agttttgtca tccaggagta caaccaggga
    69181 agcggtgctt tgactgatgc ggatcgggcc gaaacctgca tgcatgcaaa catccatggc
    69241 ggcaacggtg ttttgcgggt cgtaatgttc gtcatcgtga tgacggagaa tgcccatcat
    69301 ggtttcgagt gtgacgctgc ctttttgagt ttcaagcagg gaggtcgttg tggcgcggcg
    69361 gacacggcct ttgccgaagt tggtgtaaat aaaatcggaa taattttcgg cgtaatcaaa
    69421 ttgagatcca gatttgaccc agcctttttg tacggcattc tcgaccagtt cttttgaggc
    69481 aatatcaaac tggccgccaa gcgtaaaaca atttgagatg gaatacacat cggtgatctg
    69541 ttttgccgcc cattgtttat caacagtttc aagcagccag gtttcatttg catctgcaat
    69601 taaaaaactg ttgtggtaat acatggtttc accatgtgat gccgcgtttc caccctgacc
    69661 aaatttttcc agtaaatcta tgatgacctg cactgcctcg cgcggtgtga tggcacgttc
    69721 cagcgcagcg cgaagcatat ccattccgag caacgcaggt tttttattgg cgggcacttt
    69781 tgaatacact gcttcattac caatgacaag cccgtgttca ttcactccca tttccgcgcc
    69841 ccacatccaa aacggtttgg ataatagaac ggcgtgggtc ttttcaactt gtggaatttc
    69901 aatgtaggtg catttcaggc tgcttcctgc ggcgtgtgat tgcgcaggaa aataaacaat
    69961 atgctgacct tcgttcgggg gacggtcgga attctttgcg aagatggcaa tattatcgga
    70021 ggtggcttgt ttgctggcaa tgagagtgtc gcacatggtt attgccgaag tatagcatat
    70081 tcattttcgt ttcagatagg gtctgtccga ataattcaag gcgccagccg caaatgcacg
    70141 acaaatgttg taccttgatt caattcgctg atcacgtcaa tttttccgcc caattcatcc
    70201 acaatggatt tgacgatata caggccgatg cccgtaccag ggatttctgc aacggtgacg
    70261 ttcttggcac gataaaaacg ctcgaacaga tgcggcaccg cctcaggagg gattcccaaa
    70321 ccgtggtctg tgatggaaat acgcgcttca tgctccgcgc cgccgcaaac tgccgctaca
    70381 tcgacggtgc tgccttcggg tgagaatttc acggcattgt tgatgagatt gataaagacc
    70441 tgtgaaagac cgcccgcatc accaagcacg ctgcaaatat cagaatccac ggataattca
    70501 atggaaatat cccgcatccg cgcaatggat ttgaccgccc gcacagattc ctgaagaatg
    70561 ggagccaatt ccatgggaac ggcatcgagt ttcattcgtc cgctctcgag acggccagcc
    70621 atcaacaatt cgttgatcaa attcttttgg cggttcagtt cactggtcag cgtgtgccag
    70681 tattcgctca tttgctcttc tgtaccgcct tgctgaagca gttcggtcat cataatggcg
    70741 gcggtcaagg gggtgcggag ttcatgcgaa gcacggttga tgaaatcaga tttcatctga
    70801 tcgagccgcg cctgttcaga aatatcacgg acaatgatga gcgcctcttc ggcactgatg
    70861 ggatgcaggc gcgcctcaaa agtctgctgg tgatttggca ggttgaatcc gtacacccag
    70921 tgtgaagata gaaaggcatc ctgatcctcg gtttcgatga tcatattcac aacatcggca
    70981 ggccagagtt gatcgagccg cttccccacc accaggtcac gaggcatata caatggatgg
    71041 ctgggatgcg cgttgtaatc cagaatatgc cctgtcaggt cggtgcgcag aagcaggtcg
    71101 ggcagggcat cgaccaaaat acggttacgc atttcactaa ggcgaatggt cttttcgtag
    71161 tgcaggcgct ccaaagctgc gccgatcagg tttaccgttg tttgcacggt gtcaaactca
    71221 gccgtaagcc aggaaagctc ggtctcttta tcaaaaagcg ccagaaatcc ccttgaaccc
    71281 aaagttccct gcacgggaat gacaagaatg gaaactgtag gcacttcctg attttgcagt
    71341 tttacattgg taacggaaaa cacacgttca ggattttccg aaatcgaagc aaggaatggt
    71401 tcaagggttg aggtcagatc aaaggtggat gcgtcgtagc ttttccactg gaattgaact
    71461 ttcatggaat ttgccatgat atcgaacaag gcacagcaaa aaacatccac ggcataccca
    71521 agcgattcca acacttcggg aatcttcgtt tcgatatttg acgagcggaa cagcccagcc
    71581 gaagcttcgc caatgacgga aagcgaaagc atatggcgct tgagattcgc ttcagcctgc
    71641 ttgtgttccg aaatatcacg ggccagcatt tggatgtagg tgggcatatt gtgttcatta
    71701 tgaaccatag agatgctcaa ctctacaggc agcatcgaac catcccgctt gcgcagggtg
    71761 tattcggatg ggttcgtgcg cctctcaaga agggtctcgc gcgtttggga atcatcgatc
    71821 agcatgatcg cttccacagg cagacccacc agctggtgtt cttcatacgc cagcaatttc
    71881 aaagcctgaa gattggcagt tacgatcttg aaatcaaggc cgataatgaa tacacactcc
    71941 cctgtttgct caaataatgc gcggtaatcg aggtccgtcc ccagtaaatt tctgcctgca
    72001 tgatgcattt tcacatccac aggcagtctg ctgagaggga cagttttttc atccatattc
    72061 aacgtcttct ggccgcatat ctggcagtgt ggccacaaca caacatcctt tttcagagtc
    72121 gacgatcttt acttctccgc caagattgga gaagacagtc cgtgccacgg taagcccgac
    72181 acccaatcct tgaaattcgc gctggtcacc ctggctgatt tgataaaagc gttcaaatac
    72241 tttttcacgc agttcaacag gcacgcctac gctttcatcc tgaattgtga tggtgccgcc
    72301 cccattctca ccagacctga ccttcagact cacttgtcca tgctcggggc tgaacttgaa
    72361 ggcattatca gcgaggtgag caagcgcctg cgaaaattcc ttgcgcggcg ccttgatggg
    72421 attcgccaat ttgatatcgt acacaaagtt gattctcttc agggggtaac ggtgcagccg
    72481 tttctgtacc ggggagagaa tgtgatgctc gacatcgatc aactgtcggg ttgggttgag
    72541 atcgtgctgg tccatatccg agagcaaaat aatatccgcc accagcgtgt caagacggtc
    72601 cacactggca agtgcaatgc ggatgaactc agcctgctca tcaggatttt tatattttgt
    72661 gttgactgcc atttccaatg acatgatgac attgctcaaa ggagtgcgca attcatgtct
    72721 gaaattctgc aggacctccc tgcgaagttt atccatctcc tgctgtgccg catgctgcac
    72781 ctgctcgcgg ccgcgggctt gttcagtttg aacacggcgc aaaactgcgt caatacgcgc
    72841 cattaactcc tccgttacaa aaggtttggt tacataatca tccgcgccgc cgcgaattcc
    72901 cgccacacgg tcttcactgg ctgtacgcgc tgtaagaaaa ataaagggga tcgatgcaag
    72961 ttggggatct gtgctcattt gctttttcat atcgaatccg ttgacgacgg gcatcatcac
    73021 atcagatacg atcaaagcag gcggcgattt cttcgccttg acaatggcat cgctgccatt
    73081 catggcagtg ataacgccat agccatgacg ttcgatcgtg gctgccagcc ccatacgaat
    73141 ggcgggttca tcatccacca ctagaataat cggtttgttt tccatggtaa tttcctgatt
    73201 gataaacacc agagggtgtt tcctttctac agttcctgat tatcatcaca tcacaccgaa
    73261 atatcattgt aaggacataa cagtttcggt ttctgctttt tcaggctttc aaaggattcc
    73321 acacaatgcc agcacaaatc cagtcaatcc acccgttata atagggacat gtttatagac
    73381 cacgtaacaa tccatgtaaa atcaggcaag ggcggcgatg gaatggtgca tttccgccgc
    73441 gagaaatttg tcccgttggg tggccctgac gggggggatg gcggaagggg tggaaatgtc
    73501 atttttgaag taaaagccac gttgaacaca ctttctgcat ttcggcccaa tgaaaaattc
    73561 gccgctgaac caggtaaaaa tgggggcggt tcgcagatga caggccgtgg cggcaaggac
    73621 ctcatcattt acgtgccccc aggcactgtg ctctacgatg cagagacagg tgccctgctg
    73681 ggcgatttga ccacccccgg tcagcagctt ttggtgtgca aaggcggccg tggcgggctt
    73741 ggcaaccaac actacgccac ctcgcgcaat caggcgcccc gtacagcaga gcgcggcgag
    73801 ccccacgaag agaaaatgat ccgccttgag ctcaaactca ttgcagatat tgggatcatc
    73861 ggccttccaa atgcgggcaa atccacttta ctggcggcgt tgaccaatgc aaagccgaag
    73921 atcggtgatt atccgttcac tacgctggaa ccaaatctcg gtgtggcaaa aattgatgac
    73981 gatacaacag ttgtattggc agacatccct ggcttgatcg aaggcgcagc cgaaggtgca
    74041 ggccttggtc acgacttttt gcgtcatatt cagcgcacac gggtattgat ccatatggtt
    74101 gacggtcaat ctgaagaccc gctggccgat tacacccaaa tcaataatga acttgcgctt
    74161 tttgacccca acctggcaaa aaagccacag atcgtggcgc tcaacaaaac cgatcagccc
    74221 gatgtgcagg aacgtctgaa aagcattaag gcgagtttca aaaagaaaaa aatcgagtta
    74281 ttcacactct ctgccatggc acgcacaaac acccgcgatc tattgatcgc tgcgcatcaa
    74341 agactggccg atatgcccgt ggaagaagta gaggaaaccc tgcccgtcta caagccagat
    74401 gtggacccga atcacttcga gatcacccgc gaagacgatg gcagctggcg catcaccggc
    74461 gtggctattg agcgctcggc aaagatgacc tattttgagc acgatggctc cttgcgccgc
    74521 tttcagaagt tgctgcaacg gctcggagtg gacgacgctc tgcgtgaagc aggtgttaca
    74581 gatggcgaca ctgttttcat tggcaatttt gaactggaat ggaaggaata acttccgagg
    74641 atctgcatgg cacagctttc tcccaacaaa caacgagcgc tgacaatcgg gatgatcgtg
    74701 cttggggtca tcgtcgctgt attttttgga atgcgtgcat ttcgcgcttt caacaggttc
    74761 aacggtcatc gccccccacc gcaaggcaag gttgaaaccg atgtcgaact tatccgcggt
    74821 tggatgaccg tcccattcat ttcgcaaatg tatcgtgtcc ctgatcggat catcttcgaa
    74881 gagcttaaca tccctgaaaa gggaaacatg gaaaaaagtt tgaaggactt aaatcaggaa
    74941 tactacccca acgaggacgg ctttgtcatc gaaatggtaa aacagatcgt tcaggcgaat
    75001 ctccccaatg aaccacatga tccgccagaa ccaccaacac cgccaaaccc gtagggatct
    75061 ttgatgtctg acttctttct tacacaagtt attaattttg gtacaccact tgtcgggttg
    75121 attctttttc tgggcgcgct tggtttcccc gttggcgctt cggttgtggt gatcgcagca
    75181 ggcgccttcg gccagcaagg catattggac attccctctg cagccattta cggactgctt
    75241 ggcgcagtga tcggcgacac actcagtttt ggaatgggat actacgccaa agatgcggtc
    75301 caaaggcggt tcggaaattc agccgcatgg cagggcgcaa ctgaaacttt tcaaaaaaat
    75361 gcagggatgg cggtttttct tacgcgctgg ctcattaccg ccattgccat ccccaccaac
    75421 ctgatcgcag gcggcagcgg atataaatat tcccgcttca tttcctatga tttgacaggc
    75481 gaaatcgtgt ggatcatttt gtacggcggg ttgggttact tattcggcag tcagtgggaa
    75541 ttggtctatg attttatgag caatttcggc gggttgatcc tgggcatagt gttgatgggg
    75601 tttggcatac gccaggcgct gcaatggcaa aaaaacagca gggataaaat taaccaaaaa
    75661 gctgaggcat aagcctcagc tttttacttt ttcaacctag tgatggtgat gcccatcttc
    75721 atggacatga ccgtggtcaa gttcttcttc cgttggttcg cggagggcaa ccactttcac
    75781 ataaaacgat aactctgcac ccgccagcgg atggttgaaa tctaggcgga cggtgtcctt
    75841 gtcaaaactg gccacgtagg cagcctgatg attcccatct tcgtctgtta cgtgcaattc
    75901 catgccttct tccagcttca tatcagcagg aaattcggtg cgcggcacat ccatgaaggc
    75961 gtcatcgtca aactcgccgt agccgtcttc aggttgaacc accacatcct tgctgtcacc
    76021 gatcttcatg cccatcattt ccttttcgag ccctggaatg atgtttccat aaccaacaag
    76081 gaactgcaat ggccctgctt cgtcagacga atccaacact tcgccatcca ctgtcaattt
    76141 gtagtccatt gaaacgacca gaccatcctg tactgtatct ttgctcattc gtttttcctt
    76201 ttcttgaatt atcgcgtcgc acaatgtgac aattttttac cagatcatgc gatactgtgt
    76261 atcgcgctca ttttaccagc gaattcagcc ttcatccttg ataattcatc ctttataatc
    76321 ttttttatgc gctacgactt atgcctgccc tggtactggg aatatgacga tgctttcgtc
    76381 gggatggtgg aacgtacctg tattgaacag ggcctttccc tctggcagat caagccagat
    76441 aacctgcttg aatccatcac tgccctgtac aaaggagtga ccactttcaa aaccctgctc
    76501 caccgcggac agggtgaagc cattttcgac ccaattcccc gttgggcaaa ggaattcaac
    76561 gcgcggcgta tcaatcccgc cgaagtttcg gcatggtctg aagacaaagc caccatgcat
    76621 ctcgaattga tcagtgcagg tctgcataca cctcacacca ttttgctgcc gccttttctg
    76681 gagcaacctg ttgtgtctga aatcaatctg gcacccttgg gcgggaattt tgtcatcaag
    76741 ccatcgaatg gcggcggcgg tgaaggggtc atcattgggg caaatacgat tgaagagatt
    76801 ctacatgccc gcatggaata cccagaacaa aaatatttgc ttcaggcaca tatcactcca
    76861 cgcgtcattg aagacacacc tgcatggttc cgtgtctttt atgccaacca ggagacctac
    76921 ccctgctggt ggaacaccga gacacacgcc taccgccctg tttcagcgga ggaagagtct
    76981 cagcaccgcc tgggcaggct gcgggatgta acgaagcgga tcgcatccat ctgcaagctg
    77041 gattggtttt caacagagat cgccctcgcc gaagaattca tcgtcgtgga ttatgtcaac
    77101 gatgaaattg atacgcgggt tcaatcgcag gcgctggatg gcgtaccaga cgagatcatg
    77161 gaatgcgttg caaaacagct ggtaaaaatt gccaaggaga atgcatgaaa aatgtacgag
    77221 caagagacct ggggattcca tttgaaggac tcacaggaga gaacaatgcg atcacagacg
    77281 tttcaaatgt gaccgttggg tatgccaccg tgatcgaagg cccctcagcg cggacaggag
    77341 tgacgatcat ccacccgcgt ggagacaatg accacacacc tgtgtttgct ggcatccatt
    77401 cgttcaatgg gaacggcgaa atgacaggcg cggcatgggt ggaagaaggc ggcatgctgg
    77461 aaggccccat cggaattacg aacacccaca gcgttgggat cgtgcgcgat tccatcgtag
    77521 cctggcaggt gaaaaacgat gcgctgtatc aaaagtggtc gtgccctgta gtggccgaaa
    77581 ccgccgatgg ctggctgaac gacatgaatg gatttcatgt gaatgaaaac catgtgctcc
    77641 aggctttgaa ctccgcaaaa aacggctcaa ttgaagaagg gaacgtgggt ggaggaacgg
    77701 gcatgctgtg ctacgaattc aaaggcggaa cgggcacatc ttcacgcaaa ctgcccgcca
    77761 aactcggcgg ctggacggtg ggcgtactgg tccaagccaa cttcggcaga cgctatcaat
    77821 tgaccattgc gggcacgccc atgggacaat atttcaaaga tgatgcacca tttacgaacg
    77881 gtgaaaatcc attcaggcag gatgacggct cgttgatcgt cattgttgcc accgatgcgc
    77941 ctgtgctgcc tcatcaacta aaacgccttg caaaacgtgc caccatcggc atggcacgga
    78001 ctggcagttt gggcggcaat ggctcgggag atattttcct cgctttttcc accgccaacc
    78061 cacaagccgc acatgatggc gaaatgggcg ttgccaacat tcagtccctc tccaacaact
    78121 atatcgaccc gatccttgcg gccactgcct acgcaactga agaagccatt atcaatgcca
    78181 tggtggcagc caaagatatg aaaggccaca acgacgtgga cgccaaagca ttaccgcacg
    78241 atggcgtgcg ggaagtgcta aaacgttaca atagaattgg ttgagaaatg tctgcagtgg
    78301 aaaaagaaag aggaaagaaa gaaaaaaaaa gaagcaggtg gagaaactcc atctgcttct
    78361 tttatagatc acttacctaa aacggcaact tgcattattt atttcaccaa ccccacgatc
    78421 acatttacat tcaatttatc gccgatctct tccaactcat cgctccaggc gtggaaattc
    78481 aactttggcg gtgccagaac cacacccttc attgtgaata aaggtgttcc gctcatcggg
    78541 gctggtgtgg agcttgtatc catgttctca atgttgatgc cgcgtgaagc aagataatgg
    78601 gcaatgtcat ggatgatgcc ttcatggtca gcgcctgtca cttcgatctc atacggcagc
    78661 caaccttcat atttctttga atcctcggtc tggacaatgg atacctgata cccttcgccg
    78721 cgcaacgact gaaagtcctg gtcaatgttg cctgcttcgt tatcaggcag ggcgatcagc
    78781 atcagcatgg caaactcccc gccaaggtgt gccatgcggc tggcgcccac gttaccgccg
    78841 cgcttcacta ccaaactggt gacatggtca acaagaccga tccgatccct gcccgtcagg
    78901 gtcaatacaa tgttttttgg catggcagac tcccttttac aactggtttg tgaaatatat
    78961 taccgagtat agcataagcc caatcaacaa aaaagcccgt gattttgagn nnnnnnnnnn
    79021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    79081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gaatttttta tgctatgatt cggcatcttc
    79141 gccaaaggat agccatgcaa acactcctca aactctcgca acggatagac gccatcaccg
    79201 agtatttcgg caaagcctcc gccttctgtg ttctgctgac catcgtcatc ggcttctaca
    79261 acgtggttgc gcgctacctc ggcagattca ttggcttcaa actgaccacc aacgcactca
    79321 tcgaactgca gtggtattta ttctcgctca cattcctgct gggtttttct tacatcctca
    79381 aacacggaga aaatgtgcgc gtggattttc tctacacaaa ctggtcgcca aaaacaaaag
    79441 cctgggtgga tctggtcggc catgcgcttt tccttgtcgt tttttgtatc attggcttat
    79501 atgtcaccac ccatccagtt ctgcaatcct gggggcgcct gcccgatggc acgttcggca
    79561 cctgggaact ctcccccgac cccgatggtc tgccgcgtgc gcccatcaaa agcatgatcc
    79621 tcgttggttt tggcatgctg ctgtttcaaa caatttcaga gatcatcaaa aatattgcga
    79681 tcattcaaag gccgcaaact acagattcaa agtagacctt caacctgcaa cttgcacctg
    79741 taaccctgga ccctcacacc catgacccat tttgaatggc ttgccatcgt tatgttcgtc
    79801 ggctttttta tattcatcat gagcggatat cccgtcggct actcattcgc tgccactgcc
    79861 attctttttg gcggcattgg ctacgccctc ggcgcattcg acctcaacct gctcaaactt
    79921 ctgccaagcc gttggtttgg caccatgtcc gatttcacgt tgttggcaat tccatttttt
    79981 attttcatgg gcgcggtctt tgaaagatcg gggttggctg aagaacttct cgaaacagtc
    80041 ggcaggctca tgggtagggt cagaggcggg ctggcactgg cagtcatcct cgttggcacg
    80101 ctcctcgccg ccacaacagg tgtcgttgca gccaccatca tcatgatggg catgcttgca
    80161 ctcccaacca tgctccgaca cggctacgac aaacaactcg ccacaggcgt catcaccgct
    80221 tcaggcacat tggcgcaaat gatcccgccc agcctggtat tggtggtatt gagcgatcag
    80281 atcggcgtgc ctgttggaga tttattcctc ggggcagtga tccctggatt gttattatct
    80341 tcgctctatg ctttatacgt catcatcatc acccgcatca aacccgacct cgcacctgct
    80401 ctgccgcctg aagcattttc cgtcactgga gcagacttgc tccgaaaatc cgtgcgcgtc
    80461 atcattccgc ctgggttgtt ggtcttctct gtgctgggaa gcattttctt cgggctggcc
    80521 tcgcccaccg aagcaggcag tgtcggggct gtcggcgcct gcctgctggc attgatgaac
    80581 aaacgactca ccacaaagct cattcaagat gcagccatgt ccactgcacg gttgaccgcg
    80641 ctcgtcatca caattttatt cagctccact tttttcagcc tcgtcttcga tggccttggc
    80701 gggcaaaggc tcatcaccga cctgctggtc aacctgcctg gcggattctg gggtttcatc
    80761 atcgtagcca acattgccat ctttttactg ggtgtctttc ttgagttcgt cgaaatctgc
    80821 ttcatcgcca tgccgctttt tgtccctgcc gcgcatgcgc tcggcattga catggcctgg
    80881 ctggcaattg tcttcgccat taacctgcag accgctttca tctccccgcc tgttgggttt
    80941 tcgctgtttt acctgcaaac caccgcgcca aaagaagtgc ccacttccga catccacaaa
    81001 ggcgcattgc ccttcatggc tttgcaggtt ctcgcattaa taatcgttat tattttcccc
    81061 caaaccgtca cctggttgat cgagctctct gcaaaaagcc tgccataaaa taaaaactgg
    81121 cacaaaagcg aaaatgcttt tgtgccagtt caattcccgt ttcctgttta cagcccctac
    81181 cctgccataa acttcaacaa cgcaaactca ttggtgaagc tccacttttg aattcgagtg
    81241 cggaattcca tccatttttc atagacggcc ttgaattcag catcctctgc agatttggca
    81301 tcgtaaagtt caaaagcctt ttcctgcgcg gcctgcatga tctcatcact gtacgacatc
    81361 agcttcaccc cgcccgccag cagacgttcc aacgcctcgc cgttgaccgc gtcataatga
    81421 gccgccatgg caacattggc ctcatagctc gcggctttga tcgcttcctg atacagggca
    81481 ggcagtttgt tccactcatc cagattgatc tgcaattcaa gcgatggacc tggctcccac
    81541 caccccggga agtaataaaa gcccgtcgcc ttgtgcaagc cgagtttttc atcatcatac
    81601 ggacccaccc actccgctgc atcaattgcg cctgtttcca acgcctggaa aatctcgccg
    81661 ccggggatgt tctgcacctg cacacccaaa gccgacatta cctgcccacc caaccccggg
    81721 atgcgcattt tcaatccctg caggtcggcg agggtgttga tctccttgcg gaaccagccg
    81781 cccatttgtg cgctggtatt gccagcaggg aattgaatga cattgaattt cttcgcgtaa
    81841 aaatcctgca ataattcaag tccgccgccg ttgtagagcc acgcattttg ctgtgacgcg
    81901 gtcaaaccaa acggcaggct tgtgccaaag gccagcaccc agcttttacc aatgtaataa
    81961 taggaagccg tgtgaccgat cggcaccgca ccggacgaca ccacatccag cacttcggtg
    82021 gcttttgcaa gttctccgcc agcgcgtggt gtgatggtga atttgccgcc cgtcaacgct
    82081 ttgacacgtt cagcaaattt ctgtgttgct ccgaacaaaa tgtccaaacc tgtgctccaa
    82141 ctggtcgcca tttgccaatc cagcacgggc aggtcttcgg cggcggcatt accagcggca
    82201 ggggcgggtt ctgcacaggc ggcaagtgcg gaagccgcta cacccattcc agctactttt
    82261 aagaagtctc ttcggttcat gtcacactct ctcctttggt ttgttttgcc tgattgtatc
    82321 gtatgc
//
LOCUS       DAOQJC010000003        74461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_126_length_74461_cov_9.337513, whole genome shotgun sequence.
ACCESSION   DAOQJC010000003 DAOQJC010000000
VERSION     DAOQJC010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 74461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 74461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74461
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126_length_74461_cov_9.337513"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="PLE39_00785"
     CDS             <1..1317
                     /locus_tag="PLE39_00785"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE66886.1"
                     /translation="AKHGAKPEELVTDPNKRSETPPEWVSQAQSIGEAQSAQKAEEAP
                     IQSEAVENALNIGEQFFAEFENASKGIPSSSTTGTEPSTDAWLREFEETENQAVFSSA
                     EDSFEKQEQPRSDIPDWLNESHPSETDGQFAFEKSGSQKSDLPDWLNVEENAFPQDAS
                     AASQNDLSNWLSSLDNEPGLPFEEMPTPDSIYNASKAVSKRKPADEEPVMKKPDLPEW
                     LSNVDADDQWQAPASESTEEPVTASENENLPDWLHNLEEEEAVVVDEDSPRLVDHPVS
                     SSDWKPMANQTPEPKKEVSPVEPRKPFVPPPAEPRAEAEVQPQQMERPVEERRTEKPQ
                     SATSRKKASVSAKQRQPEAQGTSASLSQAKGELDRGDIPAALDHYKKLIKRGKHIEET
                     IRDLTESIYRYPVEVGIWQTLGDAYMRANRLKEALEAYNKAEELIR"
     gene            1381..1833
                     /gene="ndk"
                     /locus_tag="PLE39_00790"
     CDS             1381..1833
                     /gene="ndk"
                     /locus_tag="PLE39_00790"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNE66887.1"
                     /translation="MERTLVLVKPDGVQRGLIGEVIARFERRGLRLVAAKFIQVSTTL
                     AEAHYAEHKGKPFYDGLIQYITSAPVMAMVWEGSNAVAAVRQTVGSTKPAESAPGTIR
                     HDFALEVGRNLIHASDKPETGEREVALWFKKDELVDWKRAVDQWIFEK"
     gene            complement(1906..2223)
                     /gene="rsfS"
                     /locus_tag="PLE39_00795"
     CDS             complement(1906..2223)
                     /gene="rsfS"
                     /locus_tag="PLE39_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNE66888.1"
                     /translation="MVNSLEDKKGEDIVLIDLKDIVSFTDYFVLCTGTSDRMLDALAN
                     STIDNIHSAHRKKGKKQGISSDGWLVIDYGDVVVHLFSPDQREFYNLEDLWKDGKVLL
                     RLQ"
     gene            complement(2259..2843)
                     /gene="thpR"
                     /locus_tag="PLE39_00800"
     CDS             complement(2259..2843)
                     /gene="thpR"
                     /locus_tag="PLE39_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNE66889.1"
                     /translation="MSLLRVFIATEFPSNTLEAIEKQTAKLRLTLGNEIIRWVPTQNM
                     HLTLKFIGDIAASHLDFLKQLLTREAESHKPFDLQLGGLGSFPNPRRPRILWLGLHAP
                     AELLSLQKSIEAGAARLGYQQEERPFSPHLTIGRTRQNIGLPEFQKIRTALDATQLGN
                     ISPARMEAIHLFKSDLQPGGSVYTKLFSAPLSKP"
     gene            complement(2852..3232)
                     /locus_tag="PLE39_00805"
     CDS             complement(2852..3232)
                     /locus_tag="PLE39_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE66890.1"
                     /translation="MTTLQKTVMIIEDEPEAAELFGEMMRVNGFRVMKMFSSAPAIPM
                     IIQEKPDVIILDIMMPDISGLEVLRYMRREPDLATIPVIVISAKSMPSDIKTGLEAGA
                     SLYLTKPVGFHELKQAVEQVLQNA"
     gene            3362..3868
                     /locus_tag="PLE39_00810"
     CDS             3362..3868
                     /locus_tag="PLE39_00810"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNE66891.1"
                     /translation="MTDLEDFRAEKDDFFGSHPQSPLTREQRMKFKGLNYFPENESLR
                     LEVKVEEFSTKDAFEMQTSTGGVQHYERFGRFHFHVDGVEAALTIYQSPHGFFLPFVD
                     ALAGVETYPAGRYLEPEALPGGRFLVDFNVAYNPYCAYNEMWSCPITPAENRLSVAVR
                     AGEKLFHE"
     gene            complement(3920..4204)
                     /locus_tag="PLE39_00815"
     CDS             complement(3920..4204)
                     /locus_tag="PLE39_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66892.1"
                     /translation="MDNNTSLQNNQEDRNTQEQLQDEVVLKFLRVLESIQAEELSCSE
                     TFARLDEFVETEVQSKDADKITPLIREHLDMCPECNEEYEALLAVLENTK"
     gene            complement(4238..4876)
                     /locus_tag="PLE39_00820"
     CDS             complement(4238..4876)
                     /locus_tag="PLE39_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNE66893.1"
                     /translation="MTTRTNEIWLADLRANGPSHAEALNDLRDVIQKGLPYALSRWLS
                     SDNPLFQPLVEEVTQETLLRVLDQLDSFEGRSLFTTWVHKIAIRIALTELRRKRWRDA
                     SLDELTENEDAPPPPGLLADPQASPETSSERSDMLVRVRRILEEELTPKQREALVLLG
                     LQDMPMEEAAKRMKTNRNALYKLLHDARLRLKKRLALEDITPQDLLTAFEQK"
     gene            complement(5006..6433)
                     /locus_tag="PLE39_00825"
     CDS             complement(5006..6433)
                     /locus_tag="PLE39_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNE66894.1"
                     /translation="MFKIKFGTDGWRGVIAEDYTFDNVRRCAQGFATYMLSVGKKGQW
                     VIVGHDKRFSSEHFAAAVAEVLAGNGLKVYLTQEATPTPVIAYAVLDKKAAGAVNITA
                     SHNPPTDNGFKVRNETGGAIDPDGLKLIEASIPDDLKDVKRKEYKEAVAAGEIVKFDA
                     ATPYIEHLTRDGLIDLQPIKDAGLTVMVDTMWGNGAGWFPRLLAGGKTRVIEIHNERN
                     PSFPEMKRPEPIQPNIDVGLKATVDNKADVLLITDGDADRCGIGDENGEFINQLRVYA
                     LLAYYLLEVRGERGDIVKTLSTTGMLNKLGELYNVPVHETGVGFKFVAPKMTETNALI
                     GGEESGGYAFRGNVPERDGILAGLYMLDFMVKTGKKPTELLKDLFAKVGGEYFYDRVD
                     SPFSGDRAAREKIITDNNPQTLGGLKVTELITVDGFQFKLEDGGWMLIRFSGTEPILR
                     VYCETRHKDKVQGILQDGLKVAGIK"
     gene            complement(6483..7859)
                     /locus_tag="PLE39_00830"
     CDS             complement(6483..7859)
                     /locus_tag="PLE39_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66895.1"
                     /translation="MQVYLFALLAAVMVAFVYGWLNFPSLKKSGTNYYPQLAIGFKNG
                     ELSLPEQPSEALLSLSNPYNYVLRKESGVEDFPWDASLYQGRFYIYWGPVPAALLTVL
                     PVGLLNKIGDQHLVFPFVFGVFIYSLLIVVELWSRLEQRPPAWALGLALLALGIASPA
                     TLMLSRAKIYEASISASQFFFLGGCYWAFRALGSNEKNIFWQLVLAAFHWALAIGSRT
                     IILPAAAFVGVVTIFFIWKKNTAFFTPSLFAMGAPIFIVALGLCWYNWARFGSVFEFG
                     LRYQLTTTDYNEFNNLFSVRYLRENLLSYFLRPYQIQAEFPFISTVENTVSNDRLAGL
                     PYTSPHLLFLLLPLARFFGPHARAEDRQETWLINALGGASLISLVIILLYYFTAMRFS
                     FDFMPGLLVLAAIHFMQGYGQAKSNQGYLLLAVVLFSFSILLSTLIALPAVRIREVFK
                     IFELLGIR"
     gene            complement(7995..9056)
                     /locus_tag="PLE39_00835"
     CDS             complement(7995..9056)
                     /locus_tag="PLE39_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNE66896.1"
                     /translation="MSDFLNYLNTADLDTLTKTPGITRPIAGGIIAGRPFDSVDDCLK
                     VRGMGKNLLSRLQSNFEGEGNVSESRAMVTVEEEPKPAPIERSRPAQESAREEEPSFL
                     TRLGQAFLVFLRALMRLIALAIIIVGIGAAFYYGLPFINQKVIVPIEQNTVGIRELND
                     QIEILQTQLDEVNARVDAVEKTIETQSASIAQLEEMQKTLEEEMTTQNNSVMVALKRE
                     VMYTRAIETLSRARLYLSQSNFGLAKTDVQSARDLIAELSLDAPTYQVDAFNQILMRL
                     DLALGNLPAFPVIAVDDVDIAWQLMMMGLPESEAEIVPTATPLPPTLTPTVVIEPSAT
                     VTLTPAPVLEITPTPTAIP"
     gene            9135..>10267
                     /locus_tag="PLE39_00840"
     CDS             9135..>10267
                     /locus_tag="PLE39_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNE66897.1"
                     /translation="MIYLDYAATTPVDSRVFDAMLPYFRETYGNPSSVHRYGQKAESA
                     IEEAREKVAAVLHCRADEIIFTSCGSESDNLALRGAATARRSASGQKWILTSKTEHHA
                     VSKTAEQLEKEYGFSLEWLDVNEFGGVSPEALLKAVCSDTALVSVMYANNEIGTINPI
                     AELAGIAKVNNILFHTDAVQAAAYLDVNTQTLGVDLMSLGGHKFYGPKGVGALYIRKG
                     TKLLPHMTGGGQEYSLRAGTQNVPYIVGFAEALHLAAEEREMRTAHVKPLRDHIIGMV
                     LETIPDSQLTGHPTNRLPNHASFVFKNVDGNLLLQMLDAAGFACSSGSACKTGNPEPS
                     EVITALGHSRDWALGSLRITLGVDSTPEQVDSFVKELPGLVEKA"
     assembly_gap    10268..10367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10368..11858
                     /locus_tag="PLE39_00845"
     CDS             <10368..11858
                     /locus_tag="PLE39_00845"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HNE66898.1"
                     /translation="LKTQIITLESHDDLISVRDKLSWAKTPRILLVWPKYAKVTLRIL
                     DLKVLQRHADSLGAQLGLVTRRAKVRRDAESLGIPVFASTTTAQRELWPDPVPRSTRT
                     PKPPRRDLREIRDAVYQKEPAWRTSLLGRVLTFTVGVMAFVTVAGLFVPRAALTLYPE
                     TQVQSIVIPVAASEATASISLAGGVPALTLSVTEQAEQSLAIVSEISVPKSKAKGVVQ
                     FTNLSQNELSIPAGTVVATAAEVPVKFTTLEDATLAAGIDEVVEIPIEAQQAGTDGNV
                     PNDAVIVVEGPLGLTVSVTNPEPVTGGVISQVTGANDDDRARLREVMLENLRRMAETE
                     LRSKIGPADVLLVDTLEIKQVLSEAFDPPAGQPGKTLVLNMQVEFSARYVSDDAMREL
                     SLATLNAAGQDGFVSTSAPDYKVITEPSTDNSGISHFELMVTRTLLREVDEMRVFSIV
                     RGGKPDLMKAELASALSLRQSPDILLTPSWWPWMPLIPFNISVEMK"
     gene            11896..12321
                     /gene="ruvX"
                     /locus_tag="PLE39_00850"
     CDS             11896..12321
                     /gene="ruvX"
                     /locus_tag="PLE39_00850"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNE66899.1"
                     /translation="MRILAVDHGEKRIGLALSDPTATIASPLKVVEHVSRMIDAAQVA
                     EIAAQNEVGLIVIGQSYDDDGNPNPAGRRAGRFADELKKQTELPIEMWDESFSTQDAR
                     AVRIELGLSRKKRGGHQDEFAAVAILQSYLEAKRGSGNP"
     gene            12321..13484
                     /gene="mltG"
                     /locus_tag="PLE39_00855"
     CDS             12321..13484
                     /gene="mltG"
                     /locus_tag="PLE39_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNE66900.1"
                     /translation="MSDSQIVNRKSPSKNFLRRYQLHLILAVVVFCSLTCLYFALGYA
                     PARASLLYGPPASALSISDRIEYSARLLAYGDTLRSPYDPNGFEQSFFVEQGESVFSI
                     STRLEQAGLISNAQAFYDYAVYAGIDLTIQSGEYTLSPALSIIDIARALQNFSPSDAT
                     LVILPGWRMEEIAASIPTSGLEFSPEAFLALAQTPPPALEFVPSSSSMEGFFFPDSYT
                     LPRSTTPEQLLDVIARNFVQHLTDDLRTGFANQGLDIHQAVTLASIVEREAIHHEEAP
                     LIASVYLNRLAIGMKLDADPTVQYAVGYNLFQETWWTNPISAADLEFDSPFNTYLYIG
                     LPPAPISNPSLEALHAVAYPETSGFYYFRAACDNSGYHVFAATLEEQVANGCP"
     assembly_gap    13504..13603
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13619..14779)
                     /locus_tag="PLE39_00860"
     CDS             complement(13619..14779)
                     /locus_tag="PLE39_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66901.1"
                     /translation="MKIKNLLALILLLLSTLACGESTVSAAPTPACTATSPAVKLDAE
                     SRPFHMGFTRWPPEATLEGIDRMNNFVNTYGDMTALHFDGGVPWPEALTDSPLPAGLM
                     SSWQFTRDAIPAEHTLLVSITPLDMTRSKLAAYWGESDNQPLPKPWDGYSLDHADVKT
                     AYLNYARRVVEYFHPDYLAIGIEANVAQVNAPESWEAYKQLHQFVYENLKAEYPDLPI
                     FATFTISHMSGLDGGDQATQKKEIETLLPYLDMLGLSAYPYGWMYESGKADPIPDDFF
                     APALAFGKPIGITESGAPSYDFSAYGKDYKFSEEYQARWIDFLMQKGIENHFEFAIAF
                     TGIDYDKLIAGMPTDVRELATIWVYTGLQRSDGCDKFALSYWDAYLKLPFKK"
     gene            complement(14872..15606)
                     /locus_tag="PLE39_00865"
     CDS             complement(14872..15606)
                     /locus_tag="PLE39_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66902.1"
                     /translation="MSISQRLKTGFIFLLGAYLALTWLLVFFATREDRVLHAIISMGS
                     GLLLFWVVLGGSGMVLFRNRIKAFVQGIPLNWQVKFILFGILLAMTEEAITTTMTNLA
                     PLFGVAIGEAYITASANYFDVILHHSVIVFVPWFFAWAWILKRYDFSPFWVFLLLGLQ
                     GLIGESIAFGAQNLSQFAMWIFVYGLMIYLPAYTIPDGRGARKPNLLHGMLAFFLPLL
                     CGFLWAILINLIFPDHPSIHFPPIQN"
     gene            complement(15721..16725)
                     /locus_tag="PLE39_00870"
     CDS             complement(15721..16725)
                     /locus_tag="PLE39_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66903.1"
                     /translation="METFKKSLAGLFAVLFVMSAVFALLLFNFEREGFSAETYRKAFA
                     KDDFYNRLPTVLAEAMVTTDQNQLPIVMSRMDVQAWDAFFRTILAPDALTTMGDGLLN
                     STFTYLNQQSDIVQLSLAPLKTSMVGPSGVQAIYGLLSTQPSCTLMQLAQMGIDLISG
                     GSIQFCNPPAELEPVMTPAIQAQLQVAAALIPDQITVYEAPLQNDPRQKLQTFRLFMR
                     LSPILPLGFLLLMTIFAVNSFTSWLTWWGASFLATGAFAGLMSFTGAPIFSFVFQGML
                     ASRLSDYLPSIFLAYASDLATAMLQALLNPVLWQGLSLAAIGLVMLLVGYFAKASQTR
                     "
     gene            complement(16795..18774)
                     /locus_tag="PLE39_00875"
     CDS             complement(16795..18774)
                     /locus_tag="PLE39_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE66904.1"
                     /translation="MTQTVDTLFINAIVLTMDRNLNQYDPGAVAVKGDSIIAVGSEAE
                     IKSEYTASEIVDCKGRVLMPGLVNAHTHVPMTLMRGLADDLRLDVWLMGYMLPVEREF
                     VSPEFVRLGTQLACAEQIRSGVTTFNDMYFFESDIAQATAEAGMRAVCGQSVMKYPAP
                     DAPAFEDSIAYAREFIKTWKGHPLIVPAIAPHAPYSTTPEILRTCTDIAKEYDVPLHI
                     HISETAFEVENMRNEQGMPVVPYVKKQGLLEAKVIAAHCVHIDVGEMKTLQHVNAGIS
                     HNPSSNLKLASGFAPVQKMLDVGLNVGIGTDGPASNNDLDMFEEIRLASFVAKASSND
                     PTVVPAATALLMGTRLGAQALHIGHLTGSLEAGKRADLIIVDTSPAHNSPRFRRDPHN
                     AYAQLVYASKSTDVTDVMVNGKWLMRDHNLLTLNEADLIKEAQGVAKKIDTFLIEREK
                     SVLSKLIALGGSMQEESFEVQVKVKIAEPDKLVQAMKRPEIEITAQKHYQQFDDYFLF
                     DDPSQGRLRFREDNLIDEKGNSQNVRSRLTLLGQKREGEIGHDVLLSRSRFIAPAIHT
                     ARFYREYFKPAKEISITKDRLRWHIRYKNTELFINLDEIKEPPMGHFLEIKSRTWSRK
                     DADLKAELVSELLDLLGAGHAEVVTQDYIEILTAS"
     gene            complement(18839..19831)
                     /locus_tag="PLE39_00880"
     CDS             complement(18839..19831)
                     /locus_tag="PLE39_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNE66905.1"
                     /translation="MSAQRPQRKESIWVTLAWTLNLIGLGGIAVVAIGYVVMTTSFAS
                     AQEAQPISTRALPNTFTPDPNSSLFLPTVTPNPLATRITFQTPTPFVLISKNGNGIRP
                     QIIGYSVAGRPIEVYTFGNGESQRMIVAGIHGGYEWNTIALADELITHVYEHPEIIPS
                     DVTLYILRNLNPDGDARDHGYDGRVNDHGVDLNRNFPTNWAAEWDRDGCWDYRPTTGG
                     TGPGSEPETRALMGFLQSHRVEALISYHSAALGVFPGGEPWDEESTDFARALAEVTDY
                     SFPPLDLGCIFTGTLADYAVELGAVSVDMELATHTRTDFDENLAALGILLNWGR"
     gene            complement(19831..20958)
                     /locus_tag="PLE39_00885"
     CDS             complement(19831..20958)
                     /locus_tag="PLE39_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HNE66906.1"
                     /translation="MTSAEIITIGTEILLGEIVDTNTRYIARTLRSMGVDLYRTITIG
                     DNIDRIASAIHNSMERADIVITTGGLGPTVDDPTREAVAKAIGVETEFREDLWEQVVE
                     TIARYGRKPSENQKRQAYVPKGALGIRNPVGTAPCFIVETERNAVISLPGVPSEMEQI
                     LHESMIPYLQKRFNLNEIIKVRVLHCAGLGEGMIDEKIADLETLSNPTVGLAAHTGVV
                     DVRIAAKARNENEANEMIAVIEAQVRERLGNVVFGADEDKLEEVTLDLVAKRGWALTA
                     IESGLDGILARKIPHTASFSNLEPAQLLEALRAARAESKAEIALGVSVYSADRSAEMA
                     MITPRGEKTHRITYGGPPRSLPKWAMNLALNWLRMTLEEFN"
     gene            complement(21022..22179)
                     /locus_tag="PLE39_00890"
     CDS             complement(21022..22179)
                     /locus_tag="PLE39_00890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE66907.1"
                     /translation="MDTLPTSADIVIIGGGVMGASAAYHLAHRGLKKIVLIEKEKYFG
                     TGATGRCAGGVRYQFSTEINVKLSIESLPMIERFKEEIGQEVSYRPCGYLLVATNEKD
                     LAEFKHNVALQNRLGIPTQLLSGDEVRARLPLMKFNDAIAGTFNQKDGTVDPNGVVMG
                     YINAAQKMGVKAISDVEVTGVTVSGGKVDGVQTSRGGIKTRMILNVAGPWSGLIGAMA
                     GVTIPIVPLRRQMFTTNPLEEIPADFPFVIDFAKSLYFHREGEGLLVGMSNQNEKPGF
                     DQNVDDDFELVNLEAAIERLPLMEKASRASHWAGLYEVTPDAHPIYGKTDVDGFFLCS
                     GFSGHGFMHGPVSGKLMSEFILDGKFSTLDVSMLDLKRFEEGRLIQEYNVV"
     gene            complement(22252..22959)
                     /locus_tag="PLE39_00895"
     CDS             complement(22252..22959)
                     /locus_tag="PLE39_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE66908.1"
                     /translation="MLELKDVHTYYGNIHALKGISLTVNDGEIVTLIGSNGAGKSTTL
                     RTIQGLNKPRSGSVLLDGVELNKLPAHEIATLGVAQSPEGRMIFPRMTVLENLEMGAY
                     ARKDLASYKEDLEHVLTLFPRLRERISQKGGTLSGGEQQMLAMGRALMAKPKILLLDE
                     PSMGLAPLLVELIFDIIKKLNDEGTTILLVEQNALMALSIADRGYVLQTGEIVLADDA
                     KKLMKNEEVQKTYLGVE"
     gene            complement(22976..23749)
                     /locus_tag="PLE39_00900"
     CDS             complement(22976..23749)
                     /locus_tag="PLE39_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE66909.1"
                     /translation="MLFEAKAITMTFGGLTAVNKVDFNLEKGMIASVIGPNGAGKTTF
                     FNTITRVYTPTSGEMKFDGKDLSGLQPFDITTLGIARTFQNIRLFANMSALENVLVGQ
                     HCRLTSNLIGQVLRTPATMKEEADARARALDLLEFVGLRRSDANVTAKNLSYGLQRRL
                     EIARALASQPKLLLLDEPTAGMNPNETIDLTNFIQRLRKELDLTILLIEHHMRVVMAI
                     SDRVSVLDYGEKIAEGSPSEVQKNERVIEAYLGKGTATK"
     gene            complement(23761..25290)
                     /locus_tag="PLE39_00905"
     CDS             complement(23761..25290)
                     /locus_tag="PLE39_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66910.1"
                     /translation="MKLIRPGITFGIIGGLLTFLFGGWSVAVLGVLMGVGLGLGLGNN
                     FERKDPLKIALAVLPSALVAAVLLVGLSLYQNNFVMEAIGKKPAKADIAMNANLIGFF
                     GALIFTTLLASLRGLPEKQEQYAKMILLALLVIVFPFVDKATALRWTAQIIFALIFVI
                     LGLGLNIVVGYAGLLDLGYAAFFAIGAYTTGILSSPVHNIQWNFWLVIWIAAAMASVW
                     GIILGAPTLPLRGDYLAIVTLGFGEIVPVLFKNLIDVTIKEPLTCWILPMFGANTTCI
                     VFTEHFDATLGEKGISPIGRPSLPIIGEFESSNPVTWYFLIIAIILLSIFIIRRLRDS
                     RLGRAWMAIREDELAAAQMGINPVRTKLAAFAMGATFSGFAGAFYAAYIRGIFPSVFD
                     FSASIIILCVVILGGIGNINGVIVGGLVLMTADRLFLPALKDFLSSLLTHTVLPSISD
                     PVVQLAVKDNANPILYRFLLFGLTLVIMMAVRPEGLIPNAQIRAELHKEEDEASAEKK
                     G"
     gene            complement(25302..26414)
                     /locus_tag="PLE39_00910"
     CDS             complement(25302..26414)
                     /locus_tag="PLE39_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNE66911.1"
                     /translation="MENLKKINWRRLGTIAIVLYIVFLIGPIAGLDVPAIVQAIKANP
                     LQLVQFLIIGIANGAIIAIIALGYTMVYGIIELINFAHGDVYMIGVFTSLTVLSVTGT
                     LDSKDPAKIILGLILALVVAVVVCATLNATIERLAYKRLRNAPRLAPLISAIGMSFIV
                     ENIGLMWAAQAWLVPSFGTTGGASQKVFPDMLGTLFTSLGIDVNLLASSGLALRFTIK
                     DLLVIVLAVGLMTGLYLFVQRTRLGKAMRATAQNRDAAKMLGIDIDWVITATFLIGGG
                     LAGAAGLMAGLYNNTAWWFMGFRAGLQSFTAAVLGGIGNITGAMLGGFLIGLLASFSD
                     GYLNARWTNAWVFATLVLILLFRPTGLLGENTGQKA"
     gene            complement(26505..27842)
                     /locus_tag="PLE39_00915"
     CDS             complement(26505..27842)
                     /locus_tag="PLE39_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNE66912.1"
                     /translation="MNKRLLALLSVLVLASMMLAACGGGAPAATEAPAEEPAATEAPA
                     ATEAPAATEAPAAEAVTLKIVSSLPMTGSSLTQTQTIVNAMQLRLEQANYSACGGNVT
                     LEYEAWDDASAALGKWDPAVETENANKAAADPAIIAYLGTFNSGAAKLSIPILNAAGP
                     LVMISPANTNPGLTKADKDLPGVTDSYYPTGTRSYARVVAADDIQGKVAANFVKSLGA
                     TPVYILDDQELYGKGIADVFEATANEIGLTVVGHEGIDPKAADYKALMTKISTSNNGG
                     APDAIYVGMVVDNNASQLLKDKVAIMGDNETVKFVGPDGIQTQAFIDGAGADVAAGVY
                     ASVAGVPFEELPEAGKQFKADYEAKFGPLTEPYAVYGYETMNVALRAIEDVCAAGGDP
                     SDRAAINAAVFAIQNFDGALGTWSFDANGDTSLTDMTFYQVKDGAYAPVGTFK"
     gene            complement(28092..28583)
                     /locus_tag="PLE39_00920"
     CDS             complement(28092..28583)
                     /locus_tag="PLE39_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE66913.1"
                     /translation="MNSMVNFLKQGDIFYQFTPTQLELVANLCQEVVFNAGDLVFQEN
                     SGSKELYVIAQGEVDILINRGATGESDKKETAVARLRRGQSFGEVALVDEGLRSASAR
                     AAQKDTRLLVIQRDKLIMLCETYPQLGYRLMYNLAADLAMKIRNTDLRIREQLLYKPH
                     DKK"
     gene            complement(28656..30065)
                     /locus_tag="PLE39_00925"
     CDS             complement(28656..30065)
                     /locus_tag="PLE39_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNE66914.1"
                     /translation="MSKNIILLILILSLFFPFQPAQAQDTGGPIYIVQPGDSLSSIAE
                     RFSVSLADLMAVNGIANANQLNAGDALIIPGLSGITGTLNTEVVNFGDSYRSLIRRTQ
                     VSETLFKKLNHVVSPSEFYVGVSMIVPVQSGSQGLNKRISPTKGQSLLELAVQQNTDV
                     WTLSHNNHLAGSWDSMPGDTLFTVGEDNAEQTTSGLPAAFISAEIRDLPIKQGGTGVI
                     KVKTIPNVTLSGLLVDHALHFFPMEDGTQVALQGVHALLPPGVYPLRLEATLPDGSKQ
                     SFEQFILIISGNYPDDPLLYVDPATIDPASTEPELQQLIQLTLPSNPIKYWSGDFISP
                     AIAYAESTYFTSRYGNRRTYIGQGTELQITGFHTGLDFGGGDGLPITAPAAGQVVFAG
                     PLTVRGNATIIDHGWGIYSGFWHQSQINVQVGQVVQQNEVIGLVGGTGRVTGAHLHWE
                     LWVNGIQVDPLDWLILTYP"
     gene            complement(30102..30476)
                     /locus_tag="PLE39_00930"
     CDS             complement(30102..30476)
                     /locus_tag="PLE39_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNE66915.1"
                     /translation="MPRPIFVHNLNRMIEQPARVGYCDSFSCRLRGLMFRKRLELNEG
                     LLLVEKRDSRLDTSIHMFFVPFDLAVFWINSEMKVVDKIIAKSWHPAYFPKADARYTL
                     EIHPQRFGDYEIGDQVEFKNLQ"
     gene            complement(30534..30929)
                     /locus_tag="PLE39_00935"
     CDS             complement(30534..30929)
                     /locus_tag="PLE39_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE66916.1"
                     /translation="MNNTSIKHIVCIEDEPEMIELIRLILGRRGFDVHGAPGGKEGLK
                     MVRDLHPDLVLLDLMMPEMDGWEVYQQLKADSSTREIPVIVVTARAQNIDKVLGLHIA
                     KVDDYIAKPFGPQELLDSVEKVVGQKLSS"
     gene            30983..31756
                     /locus_tag="PLE39_00940"
     CDS             30983..31756
                     /locus_tag="PLE39_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNE66917.1"
                     /translation="MKIITWNVNGIRAALGKNALDWAFAQSPDFLCLQEVKARQEQLD
                     EEQLAKLKLSFVWNAAQRPGYSGVTTFFKNQPDEVVTGMGREVFDVEGRVIQTRWAGC
                     RLFNIYFPNGTRGHDRVEYKLDFYAHLLELCDALHEKGENLIITGDFNTAHMPIDLKN
                     PKQNEKTSGFLPEEREWVQKFLDHGFVDAYRKLYPEKIEYTWWTYRLDARKRGIGWRL
                     DYFLVSEKLMPFVKDVIVHDKVMGSDHCPVELVLEGEGF"
     gene            complement(31753..32673)
                     /locus_tag="PLE39_00945"
     CDS             complement(31753..32673)
                     /locus_tag="PLE39_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNE66918.1"
                     /translation="MAHSSPNDRLFLTKFAWLSIGAAIFTITLKTIAYFLTGSVGLLS
                     DALESIVNLVGAVMALAMLTIAARPADEDHAYGHTKAEYFSSGVEGALILIAAASIII
                     AAIPRLINPQPLEQVGLGLGVSIAASLANLLVALVLLQASKKHNSITLEANAHHLMTD
                     VWTSVGVLAGVGLVVLTGWERLDPIVAFVVAGNIIWSGFRIVRMSALGLMDTALEPDE
                     QKLVKNVLSSYTKNNVEYHALRTRQSGARRFVSVHILVPGKWTVQRGHRLLESIESDI
                     RKALPNVTVFTHLESLNDPASWDDTNLDRN"
     gene            32931..34793
                     /gene="ftsH"
                     /locus_tag="PLE39_00950"
     CDS             32931..34793
                     /gene="ftsH"
                     /locus_tag="PLE39_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNE66919.1"
                     /translation="MNSRSQSFFVYFLLIVAIGAMFYMGISENLNTEEPLTINEVALA
                     VQKGEVARIVIGSDDTVRVIFNNGVEEGVESRKEPDATFVDQLRSLGVTTEQLAPENV
                     VIEVSAPSVWAGVLSGAIYILPVILMAGVLWFIFRQAQGSNNAAMSFGKSRARMFSGE
                     HPTVTFADVAGAEESKQELEEIVEFLKEPQKFIQLGARIPKGVLLVGPPGTGKTLLAK
                     AVSGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRHSPCIIFIDEIDAVGRQRG
                     AGLGGSHDEREQTLNQMLVEMDGFGTDTNVIIIAATNRPDILDPALLRPGRFDRRVTL
                     DRPDVKGREAILKVHVKGKPLDPKADLASVARGTPGFVGADLENLVNEGAILAARRNK
                     KSIGQSELEEAIERVVMGPERKSRLISDEEKRIIAYHEAGHAIVANAIAEADPVQKVT
                     IVGRGQAGGVTWFRPDEDRILMSRKKMLATLAMALGGRAAEELIFDDITSGASNDIEQ
                     VTRMARAMVTRLGMSAEMGTMTYGQKEEMIFLGREISEQRDYSEAVAEQIDREVRKIV
                     EESYKIARNTVKKYRKQLDLVAKQLLEVESITREDFEKIFPAPLGKKSGTPQAV"
     gene            complement(34871..36571)
                     /locus_tag="PLE39_00955"
     CDS             complement(34871..36571)
                     /locus_tag="PLE39_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNE66920.1"
                     /translation="MNNRPWLANYDKGVPQTIDYPKAPLFHFLEEAARQYPDRACTIF
                     KGAVISYREMNALTDHMAAALVEMGVKKGDRVGIFMPNTPQFVIAYYGILKAGGAVVA
                     VNPTYPVDEIITPVNDANIEIMFTLSRFYDKLKTARERSKLKKIIVTNIKETLPPITR
                     LLFTLVKEKKEGDRVDTLASGDVWMKDLLAKHANSPKPNVEVTPDDTALFQYSGGTTG
                     VPKGAVAMHRNVVANTLQIKSWMPALEPGKEVVLMGIPLFHVYGMVAGMNFAMANGAS
                     MVMVPNARDLKDVLENISKYKATIFPGVPLLYNGINNHPDVKAGKYNLSSIKACISGS
                     APLMRETKDEFERLTGGKVFEGYGLSEAPTATHCNPLNGVNKTGSIGMPLPDVDIKLI
                     SLDDGETELPQGEIGEIIVNGPQVMKGYHNMPTETANSLRQMKDGKTWLFTGDIARMD
                     EDGYFYIVDRKKELIKPGGFQVWPREVEEAIMDHPKVLEVGVGGIPDPQRGETVKAWV
                     VVKPGETLTEAELKAFCKEHLAPYKVPTHYEFRTELPKTTVGKILRRELVRQHKEGGK
                     "
     gene            complement(36612..37547)
                     /gene="miaA"
                     /locus_tag="PLE39_00960"
     CDS             complement(36612..37547)
                     /gene="miaA"
                     /locus_tag="PLE39_00960"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNE66921.1"
                     /translation="MPLNKPPLILIVGPTAVGKTELAIQLAESLNGEIVSADSRLFYR
                     GMNIGTAKPSQEEMNRIPHHLIDIVEPDETLSLAVFQQEAKRIITEIHTRGRLPFLVG
                     GTGQYVRAVTQGWQPPEVVENPKMRDELEKTKEERGAEWLHAKLAYLDPESALKIDAR
                     NVRRTIRALEVILTTGRKFSEQRGQGEAPYRLITIGLTRPREELYQRVDERIEAMFSA
                     GFVDEVQSLLERGYAPSLPSMSAIGYRECVSVVQGQLTTEQAKVEMKRATRVFVRRQA
                     NWFKESDPEIAWFRVKEGVVMEVEKHIHNRLTFDS"
     gene            37698..38552
                     /locus_tag="PLE39_00965"
     CDS             37698..38552
                     /locus_tag="PLE39_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66922.1"
                     /translation="MINWQELTIDMFVEQLRTVYQRTYGDINSDFGRIVAWCGRLALE
                     NIANSDALYHDIDHTIMVALAGQAIVEGKHLREGGVTPRDWMHFMIAVLCHDIGYVKG
                     VCNNDTKDQFTTGVGNKMVFISPEGTDVALTPYHVDRSKQFVIERFGTGLLQDMTKFL
                     DAELIASYIEMTRFPSPPGEMYKDTKGLGGLVRAADFIGQLGDPDYLRKSPALFYEFQ
                     ELGVNEKFGYKSPNDLRKNYASFYWNSVSPYIQDALHYLRLTEDGKQWIANMHSHVFD
                     AEHDRGAR"
     gene            complement(38657..39604)
                     /locus_tag="PLE39_00970"
     CDS             complement(38657..39604)
                     /locus_tag="PLE39_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE66923.1"
                     /translation="MTEISNETRFLHAVEMGLGAWQWGDRIVWGYGQTHTDKEIRESF
                     DASLALGIRFVDTAEIYGSGYSERLLGQFLKETDQPVLVATKFFPMPWRLTKGFIPRA
                     LKGSLERLGLESVDLYQIHWPSPLMTPETMMEGMVECVQRKLTRTVGVSNFGEKRMIR
                     AYTTLARHGIPLASNQVHYSLLSREVEKNGTLARCKELGIRLIAYSPLEKGLLTGKYS
                     SENPPPGVRASQYAELIKKLPPVLKLLQEIGLNHGDKTISQVALNWLICKGALPIPGA
                     KSLQQAESNAGGAGWRMTDEEVAKLDEATDDIREYSKPA"
     gene            39786..40493
                     /gene="rnc"
                     /locus_tag="PLE39_00975"
     CDS             39786..40493
                     /gene="rnc"
                     /locus_tag="PLE39_00975"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNE66924.1"
                     /translation="MPNLNAREVESASDLSRRLGLPFSNLALLTRALTHRSFVNENPM
                     AVEDNERLEFLGDAVLDFIVGAWVYNRFPEMPEGDLTKVRSALVRNEQLAKFARKLEL
                     GNALRLGRGEAASGGSNRDNLLGSAFEALVGALFLESGLGGVEAFVHPILEDARELIL
                     NEIHDPKSKLQEWAQAQKLGAPRYRVIGTSGPDHLVTFEIAVEIAGAERGRGTGTSKS
                     HAEHAAAQDALNQLNVL"
     gene            40577..44185
                     /gene="smc"
                     /locus_tag="PLE39_00980"
     CDS             40577..44185
                     /gene="smc"
                     /locus_tag="PLE39_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNE66925.1"
                     /translation="MPLRLKSLELQGYKTFASRTVFEFASGITAIVGPNGSGKSNIAD
                     SLRWVLGEQSYTLLRGKKTEDMIFSGSEHRARAGMAQATITFDNTEQWLPVDFSEVAL
                     SRAAHRDGHNDYLINGQHVRLREMNELLAQAGLSERTYTILGQGLVDASLALKADDRR
                     RLFEEAAGVGLYRARRDEALKRLENTERNLERVLDIMAELEPRLRSLERQAKRAIDFA
                     RAQADMKLILREWYGFHWHRAQRDLTDARETVRGQEVRVRDLRAVHEKAQAEYTSFRE
                     RLSGLRAQLNGWHRQSADLHNQREAISRELAVLEERRRALTNTQASILSDQEHAMDEL
                     HLANDRFGEAEQELLRVQGEYEEAKRQLETCNLQLANRNAERATLEEQAQLLRNQIEA
                     FSQQRAENNARLDELKSRLEAQSQRIEQTKQAITSGESLAAKARSQYESAHKAREQAA
                     LALQEAEEKVISRRNEVERLERERRDALEKRTIEESDHSRLKAQLEVLQQSEQSLAGY
                     AEGARYLLDAARRDPQRLTGVRGALSAALDVPAELETAIAAALGDTLDAVLLDENQLE
                     EALGLLESDEAGRAALLPLVMQENILHHPSDENCLGIASELVNAPEEMRGAVRLALGQ
                     TLIVRNRNTARILVKDLPTHARVVTLRGEVFRGDGLIIAGKTASSSTLGRSRQKREFE
                     SALSGLVVRLAESNDLVERLSNQLAGAQRELAQADTDARESRVRLGEIQETEQQAGLE
                     SESTSRQLEWQKKQLEQLEAEAWESASTQQKITESQAEVEQRSTETLSQLKNINARLN
                     EMAVDELQDQVSYWGTRVAVAEQSVGSAAAKRDERTNEITRLDSRRVELISRLQEAEG
                     LLNNLDNEKARLRENESGLHGQIDEMRVLIEPAEKDLEMAEQEEARLQEAEAAAQRAF
                     ANAERSYGQVQLEQVRKQEALDNLHQKITDDFGLVMFDYAADVSGPVPLPLDGMVEQL
                     PVVTELAPEIEEQLTHHRAQLRRMGPINPDAKTEYDQESERYTFMKAQVEDLRKAEAD
                     LKQVVAELDEITKNEFVRTFDAVDKQFRETFVRLFGGGSARLALTDPENLVDTGIEIE
                     ARLPGRREQGLALLSGGERSLTAIALVFALLKVSPTPVCVMDEVDAMLDEANVGRFRD
                     LLIDLSKDTQFIVITHNRNTVQAADVIYGVTMGRDSASQIISLRLDQVTDDMLKRG"
     gene            complement(44352..45782)
                     /locus_tag="PLE39_00985"
     CDS             complement(44352..45782)
                     /locus_tag="PLE39_00985"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE66926.1"
                     /translation="MPNENPVLHKKHVARLQREKQQSQIILYTFIGILAAVLLLVLYG
                     YLDINYFQLKRPVAKVGETEILAKQFEARVRLQRQQLLAQYQTYSQYAQFGLDVSSQL
                     QQIQASLDSPTSIGRAVLDQMINEEIIRQEAAKRGITVSEEEISELTEGAFGYFPNGS
                     PTPSVTPTEVIMPEPPAGAFDIVTITPLPSATPENTSEPTGTATSAPAATIDPATITG
                     TPEATATLVPTATFTPTATLTVTPTLGPTNTPEPTATPYTAEGFQTEFNDANERLMEL
                     GFDDNVYKLVFENQLFETKLKDIITADVAHTEEQVWARHILVEDEATALNLIEKIKAG
                     EDFAALAQEFSTDTGSGALGGDLGWFGTGAMVPEFETAAFALENPGDFTTTPVQSQYG
                     FHIIQLIAKQERPLTADQYNAARDKVFQEWLTAAREEYGVEIFDFWQERVPSEPNFST
                     IATEAANAQDTAVAETKSAVDATAAP"
     gene            complement(46044..46331)
                     /gene="rpsR"
                     /locus_tag="PLE39_00990"
     CDS             complement(46044..46331)
                     /gene="rpsR"
                     /locus_tag="PLE39_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004648825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNE66927.1"
                     /translation="MSEERNYSGGGEGGGDRRFFAKPKFCQFCADKMLVIDYKKTDLL
                     RKYITEEGKIRPRRQTGACAKHQRVVAAAVKQARHVAFLPFSGRALDDNRS"
     gene            complement(46349..46771)
                     /locus_tag="PLE39_00995"
     CDS             complement(46349..46771)
                     /locus_tag="PLE39_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNE66928.1"
                     /translation="MSRGLNKVQIIGHLGKDPEMRYTPSGKPVTTFSVAVSRSWNSAD
                     GERHSETEWFNVVAWGNLAEICKQYLVKGQQVFVEGRLQTRRWDDKEGQKHTSVEIVA
                     NEMMMLGDRRDANNNHSQDADLSSADNGSSMAEDEFPF"
     gene            complement(46816..47100)
                     /gene="rpsF"
                     /locus_tag="PLE39_01000"
     CDS             complement(46816..47100)
                     /gene="rpsF"
                     /locus_tag="PLE39_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNE66929.1"
                     /translation="MRNYELVCVMQPDLDENAFNGVLDKVKGWISDSGGSVDKVDVWG
                     RRKLAYIIKKHREGQFVLLNVSMNPSATSDLERNLRYQETIIRHMLSVVG"
     assembly_gap    47288..47387
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<47388..48448)
                     /locus_tag="PLE39_01005"
     CDS             complement(<47388..48448)
                     /locus_tag="PLE39_01005"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HNE66930.1"
                     /translation="MSEVIVNLHMHTRYSDGSGTHQDIAKAALAAGVDAVIVTDHNVL
                     VQGFEGYYKEKNKKILMLIGEEVHDQARDPQKNHLLVFGSGRELATFAHDPQLLIDQV
                     QASGGLAFIAHPDDPAAPAFKETDISWEDWSVRNYTGIELWNALSELKTVVPTKLHGA
                     FYAFFPFFVAHQPIPNTILKWDELLATGARVVAIGGSDAHALRMSMGPISRVIFPYEF
                     HFRTVNTHLILPEGLSGDVNNDKKLIYEAMAAGHCFVGYDMPAPTRGFRFTAQGLEKT
                     AIMGDEIPAKGGVTLQAKLPSDAEIRLLKDGVVIQTWKNKSSCTHITTDAGVYRIEVY
                     RRYLGKLRGWIYSNPIYVR"
     gene            48588..48812
                     /gene="secG"
                     /locus_tag="PLE39_01010"
     CDS             48588..48812
                     /gene="secG"
                     /locus_tag="PLE39_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNE66931.1"
                     /translation="MEKFLDIALIITSVGLILGVILQSKGAGLGGLTGADTGGVFTAR
                     RGIERILFWVTIGLAVIFFGLVFAVILIGG"
     gene            48894..50585
                     /locus_tag="PLE39_01015"
     CDS             48894..50585
                     /locus_tag="PLE39_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE66932.1"
                     /translation="MKKLRWQILVVAVTVVIVALLLLSQQPISTSFLPEAAPGGIYTE
                     ALVGSMGRLNPMLDWNNPADRDINRLIFSGLIRFDSHGLPQPDLAEAWGTSPDGTLYN
                     FSIRSNAVWHDGQPVTSDDVIFTIEMIKSSGSLFPQDIKDLWSQIEIKRFDDKTFQFK
                     LPEPFAPFLDYVTFGVLPKHLLETVPADQLATAQFNLQPIGTGPYKFERLLTSGGQIV
                     GVALAVNKEYYLPSAFIEQIVFRFYPDSASALDAYQQGDVLGVSQITNDVLQQALMEP
                     TLSVYTSRLPQLGLVFLNLNNPTVAFLQSDKVRHALLLGTNRELIVSRIMQGQAIVAD
                     GPILPGSWAHYDEIEHFEYDPDAAAALLKAEGYVIPASGGDVRAKDGQYLNFTLTHPD
                     DEIHTRIAQAIQSDWALIGVRLDLQPVSYDSLVNDYLTTRNYQAALADLNTARTPDPD
                     PYLFWHQSEATGGQNYSQWDNRTASEFLESARTEADFSSRARLYRNFQVVFTKDMPSL
                     PLYYPVYSYGVDAQVQGVQVAPMYDVSDRLALVAEWYLVTRRALEEQTPVPTAAP"
     gene            50626..51993
                     /locus_tag="PLE39_01020"
     CDS             50626..51993
                     /locus_tag="PLE39_01020"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNE66933.1"
                     /translation="MSDAKKAIEYAHQNRERFTRELTEFIKIESISTDDAYKAQVLQA
                     AEWAAGHLRSIGIENVKLMPTGGHPIVYGDWIKNPGAPTVLIYGHYDVQPVDPIELWE
                     TSPFEATVKGDYLFGRGTSDMKGQVMATLNAVEAIMKTGDFPVNLKFMLEGEEEIGSM
                     NMGNFLPQHKDLFKADFCLNPDAGAISADEPTITFGLRGLAYFEVHVYGPTRDLHSGL
                     FGGTVHNPAQALAELIAGMHDANGTITLPGFYDSVRPVTDQEREATAKLPTTPESYLQ
                     QTEVPALWGEKEYTPALRTGARPTLEVNGLLSGWTGPGSKTVLPAKAMAKISCRLVPD
                     QDPAEVHKQLVRYMEEHAPKTITWEVKILHDSPWAITDVNNKGVRAMAAAMETVWGRP
                     PLYRREGGSIGVVAMLQNICGVESGLVGGGLPDDNIHSPNERMHLPTWHKNMDAFIHF
                     FFNMK"
     gene            52049..>52919
                     /locus_tag="PLE39_01025"
     CDS             52049..>52919
                     /locus_tag="PLE39_01025"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNE66934.1"
                     /translation="MEDRIITYGGQAVLEGVLMRGQKAFAIAMRAPDGSITIHTEKLA
                     KVYRSGITKVPFIRGVILLWDALGLGMKALTISANTQTGEDEKLEGPALYLTLALSMT
                     IGIGLFFLLPAGIGGAAEHYLGWSSWLNNLTEGLVRLGLLVGYVWAISFMPDVKRLFM
                     YHGAEHKTINAYEAGAELTPEVVAQYPIEHARCGTAFLLTLVLLSILVFTLLGPLPLL
                     WRLASRILFIPILAGIAIEYIRWTANHLDSPFVQFLIKPNLALQHLTTRTPDLAMLEV
                     AIQSFQSMRKAESE"
     assembly_gap    52920..53019
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<53020..54362)
                     /gene="der"
                     /locus_tag="PLE39_01030"
     CDS             complement(<53020..54362)
                     /gene="der"
                     /locus_tag="PLE39_01030"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNE66935.1"
                     /translation="MKPVVALVGRPNVGKSTLFNRLAGERLAIVDDTPGTTRDRLFGE
                     AEWNGRTFSIVDTGGIDPTHGGKTPLSVGSVEFIEDIRRQAQVAIQEADAVLFINDGE
                     TGVTEPDREVANILRRSQKKLPDGTFWPPIFLVVNKCESQERRDSASEFYEFGLGDPF
                     AISAIHGTSTGDLLDALVSSFKAPENEEEDDSIKIAIVGKPNAGKSSLLNKLTGKDRV
                     IVSPIAGTTRDSIDTKMEYEGLPITLIDTAGIRRRGRIEHGVEQYSVLRAFKSIERAD
                     VALLMIDATTGITAQDTHIAGYILDEWKSCVVLVNKWDAIEKNNETMEEYTNKIRHEL
                     NFMDYVPILFISAKTGQRMEQVLPLALQVQEERLARLTTSTINGIIHKAQDAHPHPTH
                     AGRALKMYYGTQVRSDPPTFLIYVNEPKLMHFTYIRYLENQIRAEHSFLGTPIRIV"
     gene            complement(54378..55625)
                     /locus_tag="PLE39_01035"
     CDS             complement(54378..55625)
                     /locus_tag="PLE39_01035"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNE66936.1"
                     /translation="MFRWISENYRTFLWSFALAMAVWISAVTSADPDETRTLPSAVPI
                     QVVGQNPNLVLGSELPTEMELTLRAPRSVWDVIESDPQSIRAVLDLSGLSSGEHTSEV
                     QIQIDARPVQIVSASPRTVTFLLESLDTQTFDLNLSLSGEAAIGYQIGEPVLETTEVV
                     VAGAQSQVEKVARARVQVNVSGLREDFDQTLPVEVLDEKGQTVEGVTVSPEAIHISLP
                     VSQQGGYRDVAVKVIVSGRAASGYRLTDISVYPPIVTVFASDPQLVNNLPGIVETQPL
                     SLQNAQENINTRLALNLPEGISIVGEQTVLIQAGISPIESSVTLGSEKVEIIGIDPGL
                     TAQVSPMTVDVILSGPLPLLDTLTRQGVRVTVDLTGLGIGTHQVIPKVEVLISDIVVE
                     SILPNTLEVVISLIGTPVVTPTP"
     gene            complement(55707..56582)
                     /gene="cdaA"
                     /locus_tag="PLE39_01040"
     CDS             complement(55707..56582)
                     /gene="cdaA"
                     /locus_tag="PLE39_01040"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNE66937.1"
                     /translation="MTEILNNVFFIFQRISWLSVLDIMLVTLIFFGLLYSLRDTQAMA
                     LLRGMILLVVALILLTSLVDLPAFSWFAQNSLPALLLALPVIFAPEIRRTLERLGRAG
                     TIWPASVKSTDVALEQTIRAVVSAAARLSARQHGALIIMQRLDTLDELAQSGVQMYAR
                     VTPELLLQIFYPNTPLHDGGVIIVGSQIVAAACVLPLSASGILNRTPERQMGLRHRAA
                     LGTSETSDAIAVVVSEETGAISIAHAGRMLRRLDPDRLENILTAFFRPNGKDHKPNLL
                     ERLFPGLIPTKKDKP"
     gene            complement(56742..57896)
                     /gene="coaE"
                     /locus_tag="PLE39_01045"
     CDS             complement(56742..57896)
                     /gene="coaE"
                     /locus_tag="PLE39_01045"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNE66938.1"
                     /translation="MSNWPGKFVIGLTGNIATGKSVVRKMLEHLGAYTVDADALSHRA
                     YAKGAPGYQQVIDRFGKWLLNKDGEIDRSKLGNLVFNDAEALAQLEGIVHPLVRQATE
                     MLIKRSTQNVVVIEAIKLLEGDLRKVCDSIWVTNATEDIQIERLIRKRGMNRDQARER
                     IHMQSAQSAKVAVANIVITNTGSFDDLWKQVSTAWKEIVPASVAKPADTEQTLSKVPV
                     TSNGTFTVQRGKPKHSPAIAELITRLSKGKRTVTVVDVMEDFGEKAYMLLLLDGKMVG
                     IAGWQVENLVTRTTDIFLEEHVNPQKALEILIKEVERASSELQSEASLIFPLNELAAQ
                     EELWKQLGYEKRTPETLGVQAWQDSASEALTEGGALLFKQLRQDRVLRPI"
     gene            58043..58480
                     /gene="rnpB"
                     /locus_tag="PLE39_01050"
     ncRNA           58043..58480
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLE39_01050"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     assembly_gap    58531..58630
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <58631..59489
                     /locus_tag="PLE39_01055"
     CDS             <58631..59489
                     /locus_tag="PLE39_01055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE66939.1"
                     /translation="WMKISISTGDFVHIKGKVVIVTGASSGIGEATARAFGLEGAKVV
                     LAARRVEKLQAIAQEINALNTGAETLVVPTDLSKLADIQNLIAQTLARFGRIDVLVNN
                     AGIGRVDWLENLDPVCDIQTQFDVNVMGMIQATRLALPTMIQQRSGNIINMCSIAGLV
                     ATPTYSIYAASKHAVQGFSEALRREVKPWGIHVSPIYPGGVATEFAQHAGIKRKTNAK
                     TPAFMLLTAQQVGDAIVDLVRRPRRMRIIPWLWAVTVFLNKFLPGIVDHTTINRFTIP
                     ERKDELATG"
     gene            59549..59893
                     /locus_tag="PLE39_01060"
     CDS             59549..59893
                     /locus_tag="PLE39_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE66940.1"
                     /translation="MGFLSNLFGPSVPKLGAMEVSEKMKFGKHPILVDVRQPEEFRQG
                     HIAGAKLIPLNELHKRIKELPQGREIICVCASGSRSSSAAKMLSREGYTVFDLQGGML
                     AWRHAKLPIQKG"
     gene            complement(59937..61661)
                     /gene="recJ"
                     /locus_tag="PLE39_01065"
     CDS             complement(59937..61661)
                     /gene="recJ"
                     /locus_tag="PLE39_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNE66941.1"
                     /translation="MTPDTSNTKRWVIPTSITPEADSALQAFPSILRQILFNRGYATD
                     AEARAYLNAKPDSNNDPFLMTGMREAIERIQFAIDHHEPIAIYGDYDVDGVTATALLV
                     QALEYLNADVRGYIPNRFEEGYGLNNEAMDKLQADNVKLVITVDCGIRSPNEALHART
                     LGLDLIISDHHHPAEGELPPALAVINPKQHGDIYPDKELAGVGIAYKIAEALFSIQGK
                     PLAPHLDSLLDLVALGTVADLAPLVGENRMLVRRGLRQMKRTLRQGLSSLAAVSAVKL
                     EKVNAGHIGFSLGPRLNAAGRLKEALAAFELLTTKDVFRAGELAQTLDLQNRERQRIT
                     REIQSRAEDMAMNDDPNAYLLFAAHEDFNSGVVGLAASRLTETYYRPSIVAYKGAEET
                     RGSCRSIPEFHITEALDQCADLLVRHGGHAAAAGFTVKNENLPELVSRLKTIAADKLS
                     NEDLRPTVTADAEVSLVDIRPELFENALRYLEPTGYGNPNASFVARNIKVKSARTVGS
                     DAKHLKLILEDAKGYTHDAIGFKLGDWQKKMPPRVDILFNYEVNEYNGRIGYQLNLKD
                     LKATGARD"
     gene            61750..62265
                     /locus_tag="PLE39_01070"
     CDS             61750..62265
                     /locus_tag="PLE39_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66942.1"
                     /translation="MKALAYDEKTAMVMVYTDSMLVRGEVVAKENVRVSIWLRTQGVP
                     NYIHLHGAQVISFSGGSAKNYTQAEIFIPTVQVCAFHLAPPAHDPLDYDASEINRVMQ
                     PVNIMVGSFMLRGKMRISSQTEFGTSLDVMRASWASVYEVEVSNPFLPQFNMQVPMLL
                     VNPSHVTFGLA"
     gene            62325..62398
                     /locus_tag="PLE39_01075"
     tRNA            62325..62398
                     /locus_tag="PLE39_01075"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62359..62361,aa:Pro,seq:cgg)
     gene            62559..62903
                     /gene="rpsP"
                     /locus_tag="PLE39_01080"
     CDS             62559..62903
                     /gene="rpsP"
                     /locus_tag="PLE39_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNE66943.1"
                     /translation="MVRLRLRRIGLKGQPTYRIVAADKEAPRDGRFLEILGVYNPRTN
                     PATINLKEDRIFHWMKNGALPTESVAQVFKASGTQARFERLKKGESLEALVAEAAEAQ
                     AKRAAPVKTRKD"
     gene            63020..63256
                     /locus_tag="PLE39_01085"
     CDS             63020..63256
                     /locus_tag="PLE39_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNE66944.1"
                     /translation="MKELIEYIAKSLVDHPEDVQVRESGGGSRVRIELSVSKDDMGRV
                     IGKSGKVANSIRTLLKVAAERSGKQATLDVMEPQ"
     gene            63253..63813
                     /gene="rimM"
                     /locus_tag="PLE39_01090"
     CDS             63253..63813
                     /gene="rimM"
                     /locus_tag="PLE39_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNE66945.1"
                     /translation="MTAKQKSTKQTESGSPAGEPVYLVIGYLRRSHGIHGEIIMDLHT
                     DFPERIKAGRKIFIGENHQPHTLGGVREHGNGVLVKLRGIETPEEVGRFRNQWVYVKA
                     ADVPPLPEGQYYKYELIGLDVVDDQGNALGKLNEILETGANDVYVVKNESGKEILLPA
                     IPPVILDVDMGQRVMKVHLLDGLVDG"
     gene            63838..64944
                     /gene="dprA"
                     /locus_tag="PLE39_01095"
     CDS             63838..64944
                     /gene="dprA"
                     /locus_tag="PLE39_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNE66946.1"
                     /translation="MDDRKYWVGFNLIKGIGAVRMQGLVAYFGDLASAWGADPASLAE
                     AGLGAKVIERVLAARQKVDLDQVWNKIQLQGIKILTWQDEQYPSRLKEIDQPPPVLYI
                     RGEYLLDDLFAVAIVGTRRVTPYGRQVTEEISSFLAANGITVVSGLARGVDAVAHQTA
                     LKAGGRTIGVLGSGVDKIYPPEHQKLAEQMMERGAVISDYAPGTPPDASNFPPRNRII
                     SGLSLAVVVIEAGETSGALITAEFAAEQGREIFAVPGSILAPQSKGTNKLIQNGALPL
                     LSANDLMQALDITRIGEHKTARKILPADETEARLLGVLGHEPLHVDEIRMQADLPIEK
                     VSATLALMELKGMVRQVGGMNYVSVREAQSEYKT"
     gene            65003..66094
                     /locus_tag="PLE39_01100"
     CDS             65003..66094
                     /locus_tag="PLE39_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNE66947.1"
                     /translation="MENTYAVIMAGGGGTRLWPVSRKERPKQLLPLLGQDTLFQSTVA
                     RLKNMFPYERILVVTVEEQAREMRLQAPEIPEENYIIEPAPRGTASVVGLAAAVLHKR
                     DPKAKMAILPSDHFIRNRDLFHYLLNAAFDVAENGYLVTLGITPTQPSTAYGYIQQGE
                     SLDGEYKYPAYRVKRFVEKPDELTAQKLLHTGDHSWNSGMFIWRADVILDEIDKQMPE
                     LGGALKKIAAAWGADQQGAVLDENWQTLKTETVDYGIMEHAERVAVLPAGGLGWSDVG
                     MWSSLFEVLLPDMNGNISTNSSLHLAHETHNTLVYGGNSDRLIVTIGVENMVIVDGGD
                     VVMICKTDHAQKVKDVVGHLKKHNQEKYL"
     gene            66281..>67530
                     /gene="topA"
                     /locus_tag="PLE39_01105"
     CDS             66281..>67530
                     /gene="topA"
                     /locus_tag="PLE39_01105"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNE66948.1"
                     /translation="MEAYCMKCKTKREMKDPVAGFNAKGSPVTTGTCPECGTKLYRMG
                     KTDAHADMVAPPRPEKVVVRDGKLVIVESPAKAKTVGRFLGKGYTVRASVGHVRDLLK
                     SQLSVDVENNFEPKYRVPNEKKDVVKEIKKLASTAEEIYLATDPDREGESISWHLMEA
                     AQIEPERTKRVVFHEITAPAVAEAFSHPREINMDLVNAQQARRVLDRLVGYSISPILW
                     EKVRGRLSAGRVQSVALRLIVDREREIDEFKPVEYWSIHAELKHGSAKTSFLSKLVRI
                     DDKEPELPNEETVRPILVDMETAAYAVTKVKRGERRRKPAGPFTTSTLQQEASRKLGF
                     TAKRTMGLAQGLYEGQDVGEGGTTGLITYMRTDSMNVSALAQNEAREYVSGKYGSDYL
                     PAEAPMYKTKSAGAQEAHEAIRPTS"
     assembly_gap    67531..67630
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <67631..68437
                     /locus_tag="PLE39_01110"
     CDS             <67631..68437
                     /locus_tag="PLE39_01110"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNE66949.1"
                     /translation="EENVKIPAGIAEGQEQELVRLIPEQHFTQPPPRFSEASLVQTLE
                     ENGIGRPSTYAPTISTIQQRGYVERVDKRLIPTDTGLQVNDLMVQYFPEVVDFNFTAH
                     MEEDLDKIADGEMIWTDAIREFYNPFAEDVKKAQAEMPVTKSGPEPIGRACPTCGKEL
                     VIRYGRFGKFISCSGFPECRYTEPWLEKIGVSCPKDHGDLVERKTRKGRTFFGCMNYP
                     NCDFTSWKRPIAVPCPKCSGLLVIANKREAQCTNCSESFLLEEIMPETVE"
     gene            68492..68677
                     /locus_tag="PLE39_01115"
     CDS             68492..68677
                     /locus_tag="PLE39_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66950.1"
                     /translation="MHFTPVPYLDPGSGSFIIQILIAALLGLGVALRASWGKIKGWFG
                     IKPKAEDEDGESADASN"
     gene            68664..70046
                     /locus_tag="PLE39_01120"
     CDS             68664..70046
                     /locus_tag="PLE39_01120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE66951.1"
                     /translation="MPATEQLSASFRDPSGFLFSRNGVLYRQINRAYEKDYARLMDSG
                     LYEKLVKAGLLIPHVESDQAPAESASVFKVIQPERVPFISYPYEWSFSQLKDAALTTL
                     AIHKRAIKAGMALKDASAYNIQFVRGKATLIDTLSFEIYKEGEPWVAYRQFCQHFLAP
                     LALMAYCDVRLSQLLRVYIDGVPLDLASKLLPVKTKFNFGLLTHIHVHAGAQKRYSDK
                     AVETRKGTTGVSQQAMIGLIESLEAAVKKLTWKPAGTEWGDYYENTNYTDGAFEHKRQ
                     LVREWTLEKKPTLVWDLGGNTGVFSREAVSSGAFAVSFDIDPAAVEQNYRTVRSKKEQ
                     NILPLVLDLTNPSPSLGWDHMERDSFGARGPVDMALALAVIHHLAISNNVPLPQLASF
                     FAARSKWLVIEFVPKSDSQVRRLLTSREDIFPNYTREGFESAFSNRFTIHKAEAVRDS
                     ERILYLMEAR"
     gene            70119..71114
                     /locus_tag="PLE39_01125"
     CDS             70119..71114
                     /locus_tag="PLE39_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66952.1"
                     /translation="MQFVVAFLKKLPAVPIALFVGGLVLGLVFGWVIWPTEYTNTTPD
                     SLRADLQEDYLRMAIDSYRVNLDPDLAIQRWFNLGIGADQAFKNIQANPGKLDPAVIQ
                     AYGETVTKILATGVQPPVNTGGSATLLRSIFLWGGLLVLLGIGGYGGWYMYRLLGKRG
                     SGEVTPTMQAAELNRSAEKTNFEQLGLAPPITQTMTTYVLGDDLYDESFSIDTQAGEF
                     MGEYGVGVSEAIGVGEPKKVTALEIWLFDKNDIKTATKVLMSQHAFNDPTIRARLEPK
                     GELILVEPQAQVLLETATLQLLATVVDLEYGHGPQPADSYFERITLELAIWPRQG"
     gene            complement(71161..71625)
                     /gene="greA"
                     /locus_tag="PLE39_01130"
     CDS             complement(71161..71625)
                     /gene="greA"
                     /locus_tag="PLE39_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNE66953.1"
                     /translation="MSSINYLTPEGEAKLTAELQELKGPRREELSQRLRSAIQMGDLS
                     ENADYHKAKEDQGFLEGRIQELEAILRSAVIIDNSQTKDVVSVGSRVTIQEADYDPET
                     YHLVGATEADPRKGKISIESPIGKALMEKKVGQVVEAETPGGKIKFKIISIE"
     gene            complement(71707..72855)
                     /locus_tag="PLE39_01135"
     CDS             complement(71707..72855)
                     /locus_tag="PLE39_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNE66954.1"
                     /translation="MFTRQQLEEIEDKSLASYGMRSKDSKGRAYLDSEPDYRTSFQRD
                     RDRILHTTAFRRLEYKTQVFINFEGDYFRTRLTHTLEVAQIGRTLARALGANEDLVEA
                     ICLAHDLGHSPFGHSGEVALARLMKEFGGFDHNKQSLRIVTELEQRYPEFPGLNLTWE
                     VREGMVKHESEYDISDARDFNPDLRGNLETQIANVADELAYTTHDLDDGLRSGMITPH
                     ILEGISLWEILRETYKWQGHTLSEMERHRMIRQLVGLMVTDMLQATDIRLKDSKAKTA
                     LDIQKLKYNIIGYSEEMQRRNRELKDLLYKKLYRHYRVVRMQIKAERVISELFHAYQA
                     EPSMLPDHVQFFIEKRGLERTICDYIAGMTDRYAVEEHQKLFNPSEKP"
     gene            complement(72955..73038)
                     /locus_tag="PLE39_01140"
     tRNA            complement(72955..73038)
                     /locus_tag="PLE39_01140"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73002..73004),aa:Leu,seq:taa)
     gene            73228..>74461
                     /locus_tag="PLE39_01145"
     CDS             73228..>74461
                     /locus_tag="PLE39_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HNE66955.1"
                     /translation="MADSNFYLGRLFDTKTAKVTTQPVEYDPADLTTHAVVTGMTGSG
                     KTGLCVALLEEAALQNVPAIIIDPKGDLTNLLLHFPDLLPQDFQPWIDPEIVRRAGTT
                     PERAAQEAAESWRDGLTEWGIGQDRLLALKNAAQFAIYTPGSDSGIPVSVLSSLAAPD
                     LDWETNREVLREKISSTVTALLGLVGMTDLDPLRSREHILLSNIFETHWINGKDLDLT
                     ELILQTQTPPFQNLGAFPVDTFFPPKDRMELAMVLNNILAAPAFETWREGEELDVQKL
                     LFMPDGKPRHSIFYLAHLSDGERMFFVTLLLSAVETWMRTQKGSTALRALLYMDEIYG
                     YLPPTAVPPSKGPLLRMLKQARAFGLGLLLATQNPVDMDYKALSNTGTWFIGKLQTER
                     DKNRLLDGLESAAGGIPRA"
BASE COUNT        17399 a        18588 c        19789 g        18085 t
ORIGIN      
        1 gcgaagcacg gagcaaaacc cgaagaactg gtcaccgacc cgaacaaacg cagtgaaacc
       61 ccacctgaat gggtttcgca ggctcaatca attggggaag cacaatctgc tcaaaaagca
      121 gaggaagcgc ccattcaatc tgaggctgtg gaaaacgctc tcaatatcgg cgaacaattt
      181 ttcgctgaat ttgaaaacgc ctccaaggga atcccctcct catccaccac aggaacggaa
      241 ccatccacgg atgcatggct tcgtgaattt gaagaaaccg agaatcaggc tgtattttca
      301 tctgcagaag attcgtttga aaagcaggaa caaccgcgca gcgatattcc tgattggttg
      361 aacgaatctc acccatccga aacggatggt caatttgctt ttgaaaaatc tggttcccaa
      421 aaatcggatc tgcccgattg gctgaatgtg gaagagaacg ccttccccca ggatgcatcc
      481 gccgcttccc aaaacgacct ctcgaactgg ttgagcagcc ttgataacga gcctgggctt
      541 ccgttcgaag aaatgcctac accggattcc atctacaatg cttccaaggc tgtttcgaag
      601 agaaagcctg ccgatgaaga acctgtgatg aagaagccag acctgcctga atggcttagc
      661 aatgtggacg ctgatgacca atggcaggcc cccgcgtcag aatcgaccga ggaacctgtc
      721 actgcctccg agaatgagaa tctacccgac tggctgcaca accttgaaga ggaagaagct
      781 gtcgttgtgg atgaagattc gccgcgcctg gtagaccacc ctgtatcgtc ttccgattgg
      841 aaaccgatgg cgaatcaaac ccctgaaccg aaaaaggaag tatctcctgt ggagccgcgt
      901 aaaccctttg taccgccccc cgccgagccc cgtgctgaag cggaggttca acctcaacaa
      961 atggaaagac ctgttgaaga gcgccgtacc gagaaacccc aatctgcgac ttccagaaag
     1021 aaggcaagtg tgtctgcgaa acaacgtcaa cccgaagcgc agggaacttc cgcttcgttg
     1081 agtcaggcca aaggtgagtt ggaccggggt gatattcccg ccgcgctcga ccattacaag
     1141 aaactcatca aacgaggaaa gcatatcgaa gagacgatcc gcgacctgac cgaatcgatc
     1201 tatcgatatc ctgttgaggt gggtatttgg cagacattgg gcgatgccta tatgcgagcc
     1261 aatcgtttga aggaagcact cgaggcctac aataaagccg aagagttaat tcgctgatct
     1321 gttgaatcaa actttcgttg attcgctgaa tcgtcatata ttctatttgg aggattttct
     1381 gtggaaagaa cgctcgtact tgttaagccc gatggtgtgc aacgcggcct catcggtgaa
     1441 gtgattgccc gctttgaacg tcgtgggctt cgtttggtgg ctgcaaaatt tattcaggtc
     1501 agcaccacgc tggctgaagc gcattatgcc gaacataaag gcaaaccgtt ttacgatggt
     1561 ttgatccaat acattacctc ggcgcctgtg atggcgatgg tttgggaagg ttcgaatgcg
     1621 gttgcggctg tgcgccagac cgttggttcc accaaaccag ccgagtctgc tcctggcacc
     1681 atccgccatg attttgccct tgaagtgggc cgtaatctga tccatgcttc tgacaagcct
     1741 gagaccggcg aacgcgaagt tgcgctgtgg ttcaagaagg atgaacttgt ggattggaaa
     1801 cgcgccgtgg atcagtggat cttcgaaaaa taagcgtttc acgctgatat aaaaaatccc
     1861 gcccaatttt tagttgggcg ggattttttt gtgaaaatat tttttttatt gcaggcgcag
     1921 caataccttg ccatccttcc acagatcttc aaggttataa aattcacgtt gatccgggga
     1981 gaacagatgc acaacgacat cgccatagtc aataaccagc cagccatcac tggagatgcc
     2041 ctgttttttg cctttctttc tatgggcgct gtggatgttg tcgattgtgg agttggccaa
     2101 tgcgtccaac atacggtcac tggtacccgt acacaggacg aaatagtctg tgaaggatac
     2161 aatgtctttc aagtcgatca ggacgatatc ttctcccttt ttatcttcca gggagtttac
     2221 gatttcacgg gcaagttcaa gagcgttcag ggttcacctc atggtttgga taatggtgcg
     2281 gaaaaaagtt ttgtataaac tgacccgcct ggctgcaggt cgcttttgaa tagatgaatg
     2341 gcttccattc tggcagggct gatattaccg agttgggttg catccagggc agttcgtatt
     2401 ttttggaact caggcaggcc aatgttttgt ctggtacggc caatggtgag atgcggggaa
     2461 aagggacgtt cctcttgttg atagcccagg cgggcggctc ctgcttcgat gcttttttgc
     2521 agggagagta attctgccgg ggcgtggagt ccgagccata gaatacgggg ccgcctcggg
     2581 tttggaaaag agccgagtcc gccgagctgc aagtcgaatg gtttgtgaga ttccgcttca
     2641 cgagtcagca gctgcttgag aaagtcaaga tgtgaggcag caatgtctcc gatgaatttc
     2701 aaggtcagat gcatattttg ggtgggaacc cagcggatga tttcattccc cagtgtcagg
     2761 cgcagcttgg cagtttgttt ttcaatggct tccagcgtat tggagggaaa ctcagtggcg
     2821 ataaagacgc gcaaaagact catgcgggat ttcaggcgtt ctgtaacact tgttcaacag
     2881 cttgcttgag ttcatggaaa ccgacgggtt tggtaagata cagggatgcg cctgcttcga
     2941 ggcctgtttt gatgtcactg ggcatgcttt tggctgaaat cacaatgact ggaatggttg
     3001 ccaggtctgg ttcacggcgc atgtaacgca aaacttcgag accagaaata tccggcatca
     3061 tgatatccag gatgatgaca tcgggttttt cctgtatgat catggggatg gcaggggcac
     3121 tggagaacat tttcatgaca cggaagccat taacacgcat catttctcca aataattcag
     3181 cggcttcggg ttcatcctca atgatcataa cggttttctg caaagtggtc atttttgtac
     3241 ctcatttact aaaatagcat aaactgcatg aattggcaag ggcggtctcg ttatcggaaa
     3301 tgttatcgta agaatcccgc ctgtttgcaa gtctatggtg cataacatta ttggaggaac
     3361 catgacagat ttggaagatt tccgtgcgga gaaggatgat ttctttggca gtcacccgca
     3421 gagcccactg acacgcgagc agaggatgaa attcaagggt ttgaattact tccctgaaaa
     3481 tgaatccctg cgccttgagg taaaagtgga ggaattttcc accaaggatg catttgaaat
     3541 gcagacctca acaggcggtg ttcagcacta tgaaaggttt ggcaggtttc actttcatgt
     3601 ggatggggtg gaggcggctt tgaccattta tcagagtccg catggatttt tcctgccttt
     3661 tgtagatgcc ctggcaggtg ttgagaccta ccctgctggt cgctacctgg agcctgaagc
     3721 cctgcctggc ggtcgctttt tggtggattt caatgtggcg tacaacccat attgtgccta
     3781 caatgaaatg tggtcatgcc caatcacacc tgccgagaac cgtttgtcgg tggcagtgcg
     3841 cgcaggtgag aagttgttcc acgagtagaa aagacaggtt aacaaaaaaa gcgacgggat
     3901 gttcacatcc cgtcgctttt tattttgtgt tttcaagaac ggccaaaagg gcctcatatt
     3961 cctcgttgca ttccgggcac atatccagat gttctcggat aagcggtgtg atcttatcgg
     4021 catccttgga ctgcacttcc gtttcgacga attcatcgag acgggcaaag gtttcggagc
     4081 aggatagttc ctcggcctga atgctttcca gaacccgcag gaactttaga accacctcat
     4141 cctgcaattg ttcctgggta ttgcggtctt cctgattatt ttgaagagaa gtattgttgt
     4201 ccatactgtg gatttgacga ttaatcaaat cttaatctta cttttgttca aaggcggtca
     4261 gaaggtcttg gggggtgata tcttccaagg ccagtcgttt tttcaggcgc agacgggcat
     4321 catgcaggag tttgtagagc gcgttgcggt tggtcttcat ccgtttggcg gcttcttcca
     4381 tgggcatgtc ttgcagacca agcagaacca gtgcttcgcg ctgttttgga gtgagttcct
     4441 cttccaaaat gcggcggacg cgaaccagca tgtctgaacg ctctgatgag gtttcagggg
     4501 atgcctgagg gtcagctagc aggccggggg gcgggggagc gtcttcattt tcggtcagtt
     4561 catcaaggga ggcgtcgcgc cagcgttttc gacgcagttc ggtcaacgca atacggatgg
     4621 cgatcttgtg tacccaggtg gtgaaaagac tacggccttc aaagctgtcc aattggtcga
     4681 gcacgcgcag gagggtttcc tgcgtcacct cttcgaccag cggctgaaag agtggattat
     4741 ctgaggaaag ccagcgggac agggcgtaag gcaggccttt ttggatgaca tcgcgaagat
     4801 cgttcagcgc ttcggcatgt gacggtccat ttgcccgtaa atctgcgagc cagatttcat
     4861 tggtacgtgt ggtcatgatg ttggattata agagaaaact accccgtgga cgaaaactct
     4921 tataaaactt tgagactttt tgatagcttc ttcaaaacga ccctgcctgt atatttggca
     4981 gggtcgtttt gaaatgtgaa gaaagttact tgattcctgc aactttcaag ccgtcttgca
     5041 ggattccctg caccttgtct ttatgcctgg tttcgcaata cacacgcagg atgggttcgg
     5101 taccgctgaa gcggatcaac atccagccgc catcttcaag cttgaattga aaaccatcca
     5161 cagtgatgag ctcggtgact ttcaagcctc ccaaagtctg aggattgttg tctgtgatga
     5221 tcttttcgcg ggcggcgcgg tcaccgctga acggactatc cacacggtcg tagaaatatt
     5281 cgccaccgac tttcgcgaac aggtctttca aaagctcggt tggcttcttg ccggtcttga
     5341 ccataaaatc gagcatgtac aacccggcaa ggatgccatc acgctcgggc acgtttccgc
     5401 ggaaggcgta gcctccgctc tcttcaccgc cgattagggc attcgtctct gtcatcttcg
     5461 gcgcgacgaa tttgaaaccc accccggttt catggacggg tacgttatag agctcgccga
     5521 gtttgttcaa catgcctgtt gtggacaggg tcttgacgat gtcaccgcgt tcgccgcgta
     5581 cttcaagcag ataatacgcc aacaaagcgt aaactcgcaa ttggttgata aactcgccgt
     5641 tctcgtcgcc aataccgcag cgatctgcat caccatcggt gatgagcagt acatcggctt
     5701 tgttatcaac ggtggctttc aagcccacat caatgttggg ttgaatgggt tccgggcgct
     5761 tcatttccgg gaaggatgga ttgcgttcat tgtggatttc aatgaccctg gtctttccac
     5821 ctgccaacag gcgcgggaac cagccagcgc cgttgcccca catggtgtcg accattacag
     5881 tcaaccccgc atctttgatg ggttgtagat cgatcaagcc gtcacgggtg aggtgttcga
     5941 tgtacggggt ggcggcgtcg aatttgacga tctcgccagc tgcaacagct tccttgtatt
     6001 ccttgcgttt gacatctttc aagtcatcgg gaatggaggc ttcgatcagt ttcaacccat
     6061 cggggtcgat cgctccgccc gtctcattgc ggaccttgaa gccgttatcc gtcggtgggt
     6121 tgtggctggc ggtgatgttg accgcgccag cggccttttt atcaagcacc gcatacgcaa
     6181 tgaccggagt gggagtggcc tcctgggtca ggtaaacctt caaaccattt cctgccagca
     6241 cctctgcaac ggccgcagca aagtgctcac tgctgaagcg cttgtcgtgg cccacaatga
     6301 cccactgtcc ctttttgccg accgagagca tgtaagttgc aaatccctgc gcacagcggc
     6361 gcacgttgtc gaaggtgtaa tcttctgcga tcactccacg ccaaccatcg gtaccgaatt
     6421 ttattttgaa cattggccaa ctccttggat tgaaataaat tatggcatga cttcgacgaa
     6481 ttttatcgga tgcctaaaag ctcgaatatc ttgaaaactt ccctgattct gaccgcgggc
     6541 agggcgatca atgtactcag caatatggaa aaggaaaaaa gcacgacggc caaaagcaaa
     6601 tacccttgat tggatttagc ctgcccatat ccctgcatga aatggattgc ggccagtaca
     6661 agcagcccgg gcatgaaatc gaagctgaaa cgcatggctg tgaagtaata cagcagaatt
     6721 ataaccaatg aaatgagaga tgccccaccc agggcgttga tcagccaggt ctcttgccga
     6781 tcttcagcac gcgcatgcgg accgaaaaaa cgagccaaag gcagcagcag gaacagaaga
     6841 tggggcgagg tgtaaggaag ccctgcaagc ctgtcatttg agaccgtatt ttccacggtg
     6901 ctgataaatg gaaactcagc ctgaatttga tatgggcgca gaaaatagct cagcaggttt
     6961 tcccgcaaat agcggacgga aaacagattg ttgaactcgt tgtaatctgt ggtggtgagt
     7021 tgataccgca gcccaaattc aaacacagag ccgaagcggg cccagttata ccagcacaga
     7081 ccgagcgcga cgatgaagat cggcgctccc attgcaaaca gggatggagt gaagaaggct
     7141 gtgttctttt tccaaataaa aaaaatggtc accacaccca caaatgcagc agcaggcagg
     7201 atgatggtcc gcgacccgat ggcgagcgcc caatggaatg ccgccagcac aagctgccag
     7261 aagatgtttt tttcattgct tcccagtgcg cgaaaagccc agtaacatcc gcccaaaaag
     7321 aaaaattgac ttgccgaaat tgaagcctca tagatcttcg cacggctgag catcaatgta
     7381 gctgggcttg caatgccaag tgccagcaag gccagcccga gtgcccacgc aggcggacgc
     7441 tgctcaagcc tcgaccataa ttccaccacg atcaatagag agtagataaa taccccaaat
     7501 acaaacggaa aaacaagatg ctggtcgccg atcttgttga gcagcccaac agggagcacg
     7561 gtcaatagtg ctgctggaac tggtccccag taaatgtaaa acctgccttg atacagagat
     7621 gcgtcccacg ggaagtcttc cacgccgctt tcttttcgca ggacataatt gtaggggttg
     7681 ctcaaagata gcagggcctc ggaaggctgc tctggcaggg aaagttctcc gttcttgaaa
     7741 ccgatcgcca attgtgggta atagttggtc cccgattttt tgaggctggg aaaattcagc
     7801 cagccataaa caaaagccac catcaccgct gcaagcaagg caaaaagata aacttgcatg
     7861 gaggaattca gtttggcagg tttgttcatt tggagttgat gtgaaatttg ctcgtggatt
     7921 tgcatttcaa ttcaaaatgc gggcagatta taacattttg aaaaagaacc ccgagaattc
     7981 tcggggtttc gattttacgg aattgctgtt ggggtggggg tgatttccaa aacaggcgct
     8041 ggggtaagcg taactgtagc agatggttcg atcacaactg tgggtgtgag tgtcggcggc
     8101 aggggtgtgg cggttggtac gatctcggcc tcactctcag gcagacccat catcatcaat
     8161 tgccaggcga tatccacatc atctaccgcg atgacgggga aggctggcag gttgcccagt
     8221 gccagatcaa ggcgcataag gatctggttg aacgcatcca cttgataggt cggcgcatcg
     8281 agcgataatt ctgcaatcag atcacgcgcc gattgcacat ctgtttttgc cagcccaaaa
     8341 ttactttgcg aaagatacaa cctcgcccgt gaaagagttt cgatcgcgcg tgtgtacatc
     8401 acctcgcgct tcaaggccac catcacgctg ttgttctgtg ttgtcatttc ttcttcaagc
     8461 gtcttttgca tttcttccaa ctgcgcaatg ctcgcagatt gggtttcgat cgttttctca
     8521 accgcatcca cacgggcgtt cacttcatcc agttgagttt gtaaaatctc gatttgatcg
     8581 tttagctcgc ggatccccac cgtattttgt tcgatgggca cgatcacttt ttggttaatg
     8641 aacggcagcc cgtaataaaa tgccgcacca attcccacaa taatgatggc cagagcgatc
     8701 aaacgcatca aggcgcgcag gaatacgagg aaggcctgcc ccaacctcgt caggaaggat
     8761 ggttcctcct cacgcgctga ctcttgtgca ggccgactcc tctcaatggg agcaggcttc
     8821 ggttcttcct ccactgtgac catggccctg ctttctgaaa catttccctc cccctcaaaa
     8881 ttggattgta accgtgacag caggtttttc cccatgccgc gcactttcag gcagtcatcc
     8941 accgaatcaa atggccgccc ggcaatgatc ccgcccgcga tcggacgtgt gatgccaggg
     9001 gtcttggtca atgtatcgag gtcggcggta ttaagataat tgaggaagtc actcattctg
     9061 cacctgattt cgattgattg tattttctta tctattttat caccccgata aaattgtttg
     9121 ctataatccc ttgcatgatt tatcttgatt atgccgccac aacccccgtt gactcgcgag
     9181 tctttgacgc gatgctgccg tattttcgcg aaacctatgg aaacccatct tccgtccatc
     9241 gttacgggca aaaagctgaa agcgccatcg aagaagcccg agaaaaggtg gctgctgttt
     9301 tacactgccg cgccgacgag atcatcttca cctcctgcgg ctccgaatcg gataaccttg
     9361 cgcttcgcgg cgccgccaca gcaaggcgta gcgcctcagg ccagaagtgg atcctgacct
     9421 caaaaaccga acaccatgcc gttagcaaaa ccgccgaaca acttgaaaaa gaatacggat
     9481 tttccctcga atggctggat gtgaatgaat ttggcggcgt ttcgccagag gcgctgttga
     9541 aagctgtttg cagtgacacg gcactcgtct cggtcatgta cgcgaacaat gagatcggca
     9601 cgatcaaccc catcgctgaa ttggcgggca ttgcgaaggt gaataatatt ctttttcaca
     9661 ccgatgccgt gcaagccgcg gcttatttgg atgtgaacac ccaaaccctc ggcgtcgacc
     9721 tgatgtcgct tggcgggcac aaattctacg ggccgaaagg cgttggcgcg ctctacatcc
     9781 gcaaagggac caaactcctc ccacacatga cgggtggggg gcaggaatac agcctgcggg
     9841 cagggactca aaatgtgccc tacatcgtcg gttttgctga agccttgcat ctcgcagccg
     9901 aagaacgcga gatgcgcaca gcccatgtca aacccttgcg cgaccatatc atcggcatgg
     9961 tgcttgaaac gattcccgat tctcaattaa caggtcaccc caccaaccgc ctgcccaatc
    10021 atgcttcctt tgtcttcaaa aatgtggatg gcaaccttct gttgcaaatg ctcgatgcag
    10081 cgggctttgc ctgctcttct ggctctgcct gcaaaactgg caaccccgaa cccagcgaag
    10141 tcatcacggc cctcggtcat tcacgcgatt gggcgcttgg ttccctgcgc atcactttag
    10201 gagtcgattc cacacctgag caggttgatt cttttgtgaa agaattgcct gggttggttg
    10261 aaaaagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttg aaaacccaaa
    10381 tcatcaccct cgaatcacac gatgacctca tctccgtccg cgataaatta tcgtgggcaa
    10441 aaactccgcg cattttgctg gtctggccga aatatgcgaa ggttacgctg cgcattctcg
    10501 atctcaaagt tttgcagcgt cacgccgatt cgctgggcgc acaattggga ttggtcacgc
    10561 ggcgggcaaa agtgcggcgc gatgccgaat cgctggggat tcccgtcttt gcttcgacga
    10621 cgaccgctca acgcgaactg tggcctgatc ctgttccgag gagcacacgc accccgaaac
    10681 ccccgcgccg cgacctgaga gagatccgcg atgcggtgta ccaaaaagaa cctgcctggc
    10741 gcacatccct gctgggacgt gtgctcactt tcacagtggg ggtcatggcc ttcgtaaccg
    10801 tagcaggcct gttcgtccca cgagccgcgc tgactttata tccagagacc caggttcaat
    10861 ccatcgtcat tcccgtagcg gcaagtgagg ccactgcttc catctcactt gctggcggag
    10921 tccccgcgtt gaccttgtct gtgaccgagc aggcagagca atcgcttgcc attgtcagtg
    10981 aaatttctgt gccgaaatcg aaggccaaag gtgttgtgca gttcacaaat ttgagtcaga
    11041 acgagttgag cattccagca ggcacggtgg ttgcgaccgc cgctgaagtg cctgtaaaat
    11101 ttacaaccct cgaagatgcc accctcgcag caggcattga tgaagttgtg gaaattccca
    11161 tcgaagcaca gcaggcgggc acggatggca atgtgccgaa cgatgctgtg atcgtggtgg
    11221 aagggccgct tgggttgact gtttcggtga ccaaccctga acccgtgaca ggcggtgtga
    11281 tctcgcaagt gacaggcgca aacgacgatg accgcgcaag attgcgcgag gtgatgcttg
    11341 aaaatctgcg ccgcatggcg gagaccgagt tgcgctcgaa gatcggtccc gcagatgttt
    11401 tgctggtgga tacgcttgag atcaaacagg ttctgagcga ggccttcgac ccgccagcag
    11461 gccagcctgg aaaaacgctg gtcttgaata tgcaggtcga attttcggcg cgctatgttt
    11521 cagatgatgc catgagggag ctttcactgg ctacgctcaa tgcggcgggg caggatggat
    11581 tcgtttccac ttcggcgcct gattacaagg tgatcaccga gccctccacc gataattcgg
    11641 gcatcagcca tttcgaattg atggtcacac gcaccctgct gcgtgaagtg gacgaaatgc
    11701 gcgtcttctc catcgtgcgc ggcggcaaac cagacctgat gaaagcggaa ctggcctcgg
    11761 ccttgtccct gcgccagtca ccagatattt tgctcactcc ctcctggtgg ccgtggatgc
    11821 cgctgatccc gttcaatatt tcggtggaga tgaaatgaac acaaaacagt gcgccacacc
    11881 cagcattcaa cgcctatgag aatcctcgca gttgaccacg gtgaaaaacg gatcggcctc
    11941 gctctgagcg acccgaccgc gaccatcgcc agcccattga aagtcgttga gcatgtttcg
    12001 cgcatgatcg atgccgcaca ggttgcggag atcgccgcgc aaaacgaagt cgggttgatc
    12061 gtcatcggcc agtcctatga cgatgacgga aaccccaacc cggcaggccg acgcgcaggc
    12121 cgttttgcag atgaactcaa aaaacaaacc gaacttccca tcgaaatgtg ggatgaatct
    12181 ttcagcacac aagatgcccg cgccgtccgc atcgaacttg gcctatccag aaaaaagcgc
    12241 ggcggtcatc aggacgaatt tgcagcagtg gccatcctgc aatcgtatct tgaagccaaa
    12301 cggggaagtg gaaatccgta atgagtgaca gccaaatcgt aaatcgaaaa tcgccctcca
    12361 aaaatttcct gcgccgttat caacttcacc tcattcttgc tgttgttgtt ttctgttcgc
    12421 tgacctgtct ttattttgcg ctaggctacg cccccgcgcg cgcttcactg ttgtacggcc
    12481 cgcctgccag cgcgctttcc atttcagacc gcatcgaata ttcggcacgc ctgcttgcgt
    12541 acggcgacac actcaggtca ccgtacgacc cgaacggttt cgaacaatcc ttttttgttg
    12601 agcagggcga atccgttttt tcaatatcca cccgcctcga acaggcaggt ctgatctcga
    12661 atgcgcaagc cttttacgat tacgccgtat atgctggcat tgacctgacg atccagtcgg
    12721 gcgagtacac gctcagcccc gcactttcca tcattgacat cgcccgcgcc ctgcagaatt
    12781 tttccccttc cgatgcaacc cttgtgattc ttcctggctg gcggatggaa gaaattgccg
    12841 cgtccatccc aacctcgggc ctggaatttt cacccgaagc atttctcgcc ctcgcgcaga
    12901 ctccgccccc cgcgcttgaa tttgtcccct catcctccag catggaagga ttcttcttcc
    12961 ccgattcgta taccctgccg cgctccacaa cccccgagca actgctggat gtcatcgcgc
    13021 ggaatttcgt ccagcacctc accgatgatt tgcggacagg ctttgccaat cagggccttg
    13081 atatccatca ggcggtgacc ctcgcctcca tcgtcgaacg tgaggccatt catcacgaag
    13141 aagctccgct gatcgcttct gtgtacctga accgccttgc catcggcatg aaactcgatg
    13201 ccgacccaac cgtgcaatat gcagtggggt acaacctttt tcaagaaacc tggtggacga
    13261 atcccatcag cgccgccgac ctcgaatttg actcgccttt caatacctat ctgtacattg
    13321 gcttgccgcc tgcgccgatc tccaatccca gcctcgaggc cttgcatgcg gtagcctatc
    13381 ctgaaacctc tgggttttat tacttccgcg ccgcctgtga caactcaggc tatcacgtct
    13441 tcgccgccac acttgaagag caggtcgcca acgggtgtcc gtaaaaatag ccgcgaattt
    13501 cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttcgcgg ctatgatttt
    13621 actttttgaa cggcaatttc aaatacgcat cccaatatga aagtgcaaat ttgtcacaac
    13681 cgtcactgcg ctgcaaccct gtgtaaaccc aaatggtggc cagctcgcgc acatctgtgg
    13741 gcattccagc gatcagcttg tcgtaatcaa tgcctgtaaa cgcgatggcg aattcgaaat
    13801 ggttttcgat tcccttctgc atcagaaaat caatccaacg cgcctgatac tcttcgctga
    13861 atttgtaatc cttgccgtag gcagaaaaat cataagacgg tgcgcccgat tcggtaatgc
    13921 cgatcggttt tccaaaggcc agtgcgggtg caaagaaatc atcggggatc gggtcagcct
    13981 tgccgctttc gtacatccaa ccgtagggat aggcgctcaa tccgagcata tccagatatg
    14041 gcagcagcgt ttcgatctct ttcttttgtg tggcttggtc gccgccatcc aacccgctca
    14101 tgtggctgat cgtgaatgtg gcaaaaatgg gcaggtcggg atattccgct ttcaggtttt
    14161 catacacaaa ttgatgtaat tgcttgtaag cctcccaact ctcgggtgcg ttgacctgtg
    14221 ccacattcgc ttcgatgcca attgccagat aatcggggtg aaaatattcc accacacggc
    14281 gcgcataatt caaataggcc gtcttcacat ctgcatggtc cagcgagtag ccatcccacg
    14341 gcttgggcag cggctggtta tcagattcgc cccaatatgc tgccagcttg gagcgggtca
    14401 tatccagcgg cgtgatcgaa accaacagag tgtgctccgc tggaattgca tcgcgtgtga
    14461 attgccaact gctcatcaac cctgcgggca ggggagaatc ggtcagcgct tcgggccacg
    14521 gcacaccgcc atcaaaatgc agggcggtca tgtcgccata agtgttcacg aagttgttca
    14581 tgcggtcgat tccttccaat gtcgcttcgg gaggccagcg cgtgaaaccc atgtgaaatg
    14641 gacgagattc cgcatccaac ttaacggcgg ggctggttgc tgtacaagcg ggtgtcggcg
    14701 ccgctgatac agtcgactct ccgcaggcca gtgtggatag caatagcaag atcagagcaa
    14761 gcaggttttt tattttcatc ttattttttt tctcctgaaa agaatcccat atttcaagcc
    14821 atgcatgagc gcaattgccg cgatcaaatt gcgtttcatg tgactccgaa attagttttg
    14881 gatgggtgga aagtgaattg aaggatggtc tggaaagatc aggttgatca aaatcgccca
    14941 cagaaaacca caaagcagcg gaagaaaaaa tgccagcatt ccgtgtaaaa gattcggttt
    15001 tcgcgcacca cgcccatcag ggatcgtgta agcgggcagg tagatcatca gcccgtagac
    15061 aaagatccac atggcgaatt gacttaggtt ctgcgcgcca aatgcgatgg attcaccgat
    15121 gagtccctgc aatcccaaca gcaggaacac ccaaaatgga ctgaaatcgt agcgcttcaa
    15181 aatccatgcc caggcgaaga accacgggac aaaaacgatc acgctgtgat gcagaatgac
    15241 atcaaagtaa ttggccgagg cggtgatgta ggcttccccg attgccacgc caaagagcgg
    15301 cgcaaggttg gtcatggtgg tggtgatggc ttcttcggtc atggccagta aaatgccaaa
    15361 aagaatgaat ttgacctgcc aattcagcgg aatgccctga acgaaggctt ttatcctgtt
    15421 tcggaacaga accatacccg agccgcccag caccacccaa aataaaagca gacccgaccc
    15481 cattgaaata atggcatgaa gtacacggtc ttcccgcgtg gcaaaaaaga ccagaagcca
    15541 ggttagggca aggtatgctc ccagtagaaa aataaaacct gttttcaagc gttgtgaaat
    15601 ggacatatct atcactgata caggccaaat gccgaatttg ttgcaggaat caaaaacggg
    15661 attcgctttg cttcgaatcc cgttttctgg ttttggaatg tgggctggag ttcgagttct
    15721 ttagcgtgtt tggcttgctt ttgcaaaata tccgaccagc agcatcacca gaccgatggc
    15781 ggccaaactc aatccctgcc agagcacagg attgagcaat gcctgcagca tggcagtggc
    15841 aaggtcactg gcgtaggcca ggaaaatgga tggaagataa tcggataatc tggaggccag
    15901 catgccttga aaaacaaaac tgaaaatggg ggcacccgtg aagctcatca aacctgcaaa
    15961 cgcgcctgtc gccaaaaagg aagcacccca ccaagtcaac caactggtga aggagttgac
    16021 cgcaaagatc gtcatcaaga gcaggaagcc cagcggcaaa atggggctga gccgcatgaa
    16081 taaccgaaag gtctgcagtt tctggcgtgg atcattttga agcggcgctt cgtacacggt
    16141 gatctggtcg ggaatgagcg cagcagccac ttgtagttgt gcctgaatgg ctggcgtcat
    16201 cacgggttcc aactcggcgg gtgggttgca aaattggatg ctcccgcccg agatcaggtc
    16261 aatgcccatt tgggcaagct gcatcagggt gcagctgggt tgggtgctca aaagcccgta
    16321 aatggcctgc acgcccgaag ggccgaccat gcttgtcttt agcggagcaa gcgaaagctg
    16381 gacaatatcc gattgttgat tcaagtaggt gaaggtcgag tttaatagtc catctcccat
    16441 ggttgtaagt gcatcgggtg cgagtatggt gcggaaaaat gcatcccagg cttgcacatc
    16501 cattcggctc atcacaatgg ggagttgatt ctgatcggtg gtgaccatgg cttcagcgag
    16561 gaccgtgggg agcctgttgt aaaaatcatc ttttgcaaag gctttgcggt aggtttcggc
    16621 tgaaaagccc tcacgctcaa aattaaaaag cagcaacgcg aaaaccgcgc tcatcacaaa
    16681 gagcaccgca aaaagacctg ccagagattt tttgaaagtt tccatatttt atttttgatt
    16741 acatagacaa acggcagacc acaatccgtg gcctgccgtc agacatgcaa ttccttagct
    16801 ggctgtcaaa atttcgatgt agtcttgcgt gactacttct gcgtggcctg cgccgagcag
    16861 gtcaagtaat tcgctgacca attcagcctt gaggtcggcg tctttgcgac tccacgtgcg
    16921 gcttttgatt tcaaggaagt gccccatggg cggctccttg atttcatcga gattgatgaa
    16981 tagttcggtg tttttatagc ggatgtgcca gcgcaggcgg tcttttgtga tggagatttc
    17041 ctttgcaggt ttgaaatact cacgataaaa acgcgctgtg tgaatggcag gcgcaatgaa
    17101 gcggctgcgc gaaagcagca catcatggcc gatctcgcct tcgcgttttt gcccgagcag
    17161 ggtcaggcgc gagcggacat tttgcgaatt ccctttttcg tctatgaggt tgtcttcgcg
    17221 gaagcgcaga cgtccctggg atggatcgtc gaagaggaag taatcgtcga actgctggta
    17281 atgcttctgg gcggtgatct cgatctcggg gcgcttcatt gcctggacca atttgtctgg
    17341 ttctgcgatc ttgaccttga cctgcacctc gaaagattcc tcctgcatcg agccgccaag
    17401 cgcgatcaat ttcgaaagca ccgatttctc acgttcgatc aggaaggtat cgatcttctt
    17461 tgcaacaccc tgggcctctt tgatcaagtc tgcttcgttg agggtgagca ggttgtggtc
    17521 acgcatcaac cacttgccgt tgaccatcac atcggtcaca tcagtggatt tggaggcgta
    17581 gaccagttgg gcgtaggcat tgtgcgggtc acggcgaaaa cgcggggagt tatgcgcggg
    17641 ggatgtatcg acgataatca ggtcggcgcg tttgcctgct tccagcgacc ctgtcaaatg
    17701 tccgatgtga agcgcctgag ccccgaggcg ggtacccatc agtaatgcag tggccgccgg
    17761 gacgacggtg gggtcattcg aagaagcctt cgccacaaaa gaagcgaggc ggatctcttc
    17821 gaacatgtcg aggtcgttgt tggaggcggg gccgtcggtg ccgatgccta cattgaggcc
    17881 gacatcgagc attttttgca cgggtgcaaa ccccgaagca agtttgagat tcgaagatgg
    17941 gttgtgtgag atgcccgcat ttacatgctg gagtgttttc atttcgccaa catcaatgtg
    18001 gacacaatgc gcggcgataa ccttcgcttc aagcagtccc tgctttttca cataaggcac
    18061 gacgggcatg ccctgttcgt tgcgcatgtt ttccacttcg aatgcggttt ctgaaatatg
    18121 gatgtgcagc ggcacatcgt attcttttgc aatgtcggtg caggtgcgta aaatttcggg
    18181 ggtggtggag tagggggcgt gcggcgcaat ggctgggacg atgagcgggt gtcccttcca
    18241 ggtctttata aattcacgcg catatgcaat ggaatcttca aaagcgggcg catcgggtgc
    18301 gggatatttc atcaccgatt ggccgcaaac tgcgcgcatg cctgcttcgg ctgtggcctg
    18361 tgcgatgtcc gattcaaaga agtacatgtc gttgaatgtg gttacgccgc tgcggatctg
    18421 ttcggcgcag gccaattggg tgccgagacg cacaaattca ggcgaaacaa attcacgttc
    18481 cacgggcagc atgtaaccca tcagccagac atcgaggcgc aggtcgtcgg caaggccgcg
    18541 catgagggtc atgggcacat gcgtatgggc gttgaccagc cccggcatga gcaccctgcc
    18601 tttgcaatca acgatctcgc tggctgtata ttcacttttg atttcggctt cgctgcccac
    18661 agcgatgatc gaatcgcctt tgacggcgac tgcgccgggg tcatattgat tcaagttccg
    18721 atccatcgtg aggacaatgg cattgataaa tagtgtatct acggtttgtg tcattcattc
    18781 tccttttgca agtgatctgt ttttggagtg cgaaattttt ccgagcttgt ttctgatttt
    18841 atctgcccca gttcaaaaga attcccaagg cggcgaggtt ttcatcgaaa tctgttcggg
    18901 tgtgtgtggc gagttccatg tccactgaaa cggcgccgag ttccacggca taatcggcga
    18961 gggtgcctgt gaaaatgcat cccagatcca gcggtgggaa ggagtaatcg gtcacttccg
    19021 cgagggcgcg ggcgaaatct gtggattctt cgtcccaagg ttctccaccg gggaaaactc
    19081 ccagcgcagc actatggtaa ctgattagtg cctccacgcg gtgcgattgc aggaaaccca
    19141 tcaacgcgcg ggtttcaggt tcagagccag ggcctgtgcc gcctgtggtg gggcggtagt
    19201 cccaacagcc gtcacggtcc cattctgccg cccagttggt ggggaagttt cgattcaagt
    19261 ccacgccatg gtcgttgaca cggccatcgt acccgtggtc acgtgcatca ccgtcggggt
    19321 tcaaattgcg caggatgtaa agcgtgacat cactggggat gatctctggg tgttcataaa
    19381 cgtgggtgat gagttcatca gccagcgcaa tggtgttcca ttcgtagccg ccgtgaatgc
    19441 cagccacgat catgcgctga ctttcaccgt tgccaaaggt gtatacctct atggggcgcc
    19501 ctgcaaccga atagccaatg atctgcggac gaattccatt tccatttttg ctgatgagca
    19561 caaagggagt cggcgtttgg aatgtgatgc gcgtggcaag cggatttgga gtgacggttg
    19621 gcaggaacag cgaggaattt gggtcaggtg tgaaggtgtt gggcagggcc cttgtagaaa
    19681 tgggctgtgc ctcctgcgca gaggcaaagg aagtggtcat gaccacatac ccaatggcaa
    19741 caacggcgat gccgcccaga ccgattagat tcagcgtcca tgccagtgtg acccaaatgg
    19801 attctttgcg ttgtgggcgc tgtgcagaca ttagttgaat tcctcaaggg tcattctcaa
    19861 ccagttcaag gccaggttca ttgcccattt gggcaggctg cgcggcgggc cgccgtaggt
    19921 gatgcggtgg gttttctctc cacgcggcgt gatcatcgcc atttctgcgg agcggtctgc
    19981 gctgtagacg gaaactccca atgcaatctc tgcttttgat tctgcccggg cggcgcgtaa
    20041 tgcttcgagg agctgggcag gttcaaggtt ggagaaagaa gcggtgtgcg gaatcttccg
    20101 cgcgagaatt ccatccagcc ccgattcgat ggcggtcaag gcccatccgc gttttgctac
    20161 aaggtcgaga gtcacttctt ccaatttatc ttcatctgcg ccaaagacca cattgccgag
    20221 cctttcgcgc acctgcgctt cgatgacggc gatcatttca ttggcttcgt tctcgttcct
    20281 ggcttttgcg gcaatgcgta catccactac gcctgtgtgt gccgccagcc ccacggtggg
    20341 gttgctgagg gtttcgaggt cggcaatctt ttcatcgatc atgccttcac caagcccggc
    20401 acaatgcagc acgcgtactt taatgatctc gttgaggttg aagcgttttt gcaaataggg
    20461 gatcatggat tcgtgcagga tctgttccat ttcactgggc acaccgggca gggaaatgac
    20521 agcattgcgt tcggtttcca caatgaaaca gggggccgtg ccaacagggt ttctgatgcc
    20581 aagggcgcct ttgggcacgt aggcctgccg cttttgattt tcggagggtt tgcgcccata
    20641 gcgagcgatg gtttccacca cctgctccca aaggtcttca cggaattccg tttccacgcc
    20701 gatagctttg gcgactgctt ctcgggtggg gtcatctacg gtggggccaa gcccgcctgt
    20761 ggtgatgacg atgtcggcac gttccatcga gttgtggatc gcgcttgcaa tgcggtcgat
    20821 gttatcgcca atggtgatgg tgcgatacag atccacaccc atgctgcgga gcgtgcgcgc
    20881 aatataacgc gtgttggtat ccacgatttc gccgagtaag atttccgttc ctatggtgat
    20941 gatctctgct gatgtcattt tgtctcacat aagtttctga tatgaaaaag gatgcaaaca
    21001 tccttttgtg caggtggtta attaaaccac attgtactct tgaattaatc ggccttcttc
    21061 gaagcgtttt agatccaaca tggaaacatc gagggtggag aactttccat ccaaaataaa
    21121 ctcgctcatc aatttacccg aaacaggtcc atgcataaaa ccatgcccgg agaatccaga
    21181 acacagaaaa aaaccatcca catcagtttt tccgtaaatc gggtgtgcat cgggcgtcac
    21241 ttcatacagg ccagcccaat gcgaagcccg actggccttc tccattagtg gcagccgttc
    21301 aattgccgct tcaagattca caagctcaaa gtcatcgtcc acgttttggt cgaagccagg
    21361 tttttcgttc tggttgctca tgcccaccag caagccttcg ccttcgcgat gaaaatacag
    21421 cgattttgca aaatcaatta caaagggaaa atcggcgggg atttcctcca atgggttggt
    21481 ggtgaacatt tgcctgcgaa gagggacaat ggggatcgtg acacctgcca tcgctccgat
    21541 caaacccgac caaggccctg ccacgttcag gatcatgcgc gtcttgattc cgccccggct
    21601 tgtttgcaca ccgtcaacct tccctccgct gacggtgact cctgtaactt caacatccga
    21661 gatggctttt acccccatct tttgtgcggc attgatgtat cccattacca cgccatttgg
    21721 gtcaacagtg ccatccttct gattaaatgt ccctgcaatt gcatcattga atttcatcaa
    21781 cggcaatcgc gcacggactt catctccgct cagcaattgg gttggaatgc caagcctgtt
    21841 ttgcagggcc acgttatgtt tgaattcggc aaggtccttt tcgttcgttg ctacaagcag
    21901 gtatccgcaa ggacgatagc tcacttcctg cccgatctct tccttgaagc gttcgatcat
    21961 cggcaggctt tcgatggata gttttacatt gatctctgtt gaaaattgat agcggacgcc
    22021 gcctgcgcat cggcctgtgg cgcctgtgcc aaaatacttt tccttttcaa tcagaacgat
    22081 ttttttaagt ccgcggtggg cgaggtgata ggctgcgctg gcgcccatca cgccgccgcc
    22141 aataatgaca atgtctgcag aagtgggtag tgtatccatg agatgtgcct ttattttgaa
    22201 aagacccgct tttgcaagcg ggtctttgac agtacatgag cttctatgtg tttattccac
    22261 gccgaggtag gtcttttgaa cttcctcgtt cttcattagc tttttagcgt catctgccag
    22321 aacgatttca cctgtctgca gcacgtagcc gcggtctgca atggaaagtg ccatcagggc
    22381 gttctgttcc accagcagga tggtggtgcc ttcgtcgttt agcttcttga tgatgtcgaa
    22441 gatcaattcc accagcaacg gagcgagacc catcgaaggc tcgtcaagca gaaggatctt
    22501 tggctttgcc atcaacgcac ggcccatggc gagcatttgc tgttcaccgc cagagagggt
    22561 gccgcctttc tggctgatac gttccctaag gcgtgggaag agggttaaca catgttcgag
    22621 gtcttctttg taggaggcca gatccttgcg ggcataggca cccatctcaa ggttttccag
    22681 cacggtcatg cgcgggaaga tcatgcgccc ttcgggactc tgcgccacgc cgagtgtggc
    22741 gatctcatgc gcgggaagtt tgttcaattc tacgccatcg agaagtaccg atccgctgcg
    22801 tggtttgttc agcccttgaa tggtgcgcag tgtggtgctt tttcctgcgc cgttcgagcc
    22861 gatcaatgtg acgatttcgc cgtcgttgac cgtaagcgaa atccctttca aggcgtggat
    22921 attaccgtag taagtgtgga catcttttaa ttcaagcatg ttgtgttcct cgcctttatt
    22981 ttgtggcagt gcccttgccc agataggctt cgatcacgcg ttcattcttc tgaacttcgg
    23041 aaggcgaacc ttctgcgatc ttttcgccat agtccagcac tgaaacgcgg tcagagatgg
    23101 ccattaccac gcgcatatga tgttcgatga gcaggatggt caggtcaagt tccttgcgca
    23161 ggcgttgaat gaagttggtc agatcaatgg tttcattcgg attcatgcct gctgttggct
    23221 cgtcgagcag gagcagtttg ggttgtgaag caagcgctcg ggcgatctcg aggcggcgct
    23281 gcaaaccgta ggacagattc ttggcggtca cattcgcgtc actgcggcgc agtcctacaa
    23341 attcaagcag atctaatgcg cgcgcgcggg catcggcttc ttctttcatg gttgcgggag
    23401 tgcgcaaaac ctgcccgatc aggttggagg tcaggcggca atgctggccg accagaacgt
    23461 tttccaaagc cgacatattc gcgaataggc ggatgttttg gaaggtgcgg gcgattccta
    23521 gcgtggtgat atcgaacggt tgcaggccag aaagatcctt gccatcgaat ttcatttcac
    23581 cgctggtagg tgtgtaaacg cgggtgatgg tattgaagaa ggttgttttg cctgcgccgt
    23641 tcgggccgat cacactggcg atcatgcctt tttcgaggtt gaaatcaacc ttgttcacgg
    23701 cggttaaacc gccgaatgtc atggtaattg ctttggcttc aaaaagcatg gcagtctctc
    23761 ctaccctttc ttttctgcgg atgcttcgtc ttcttccttg tgcaattccg cgcggatttg
    23821 tgcatttgga atcaaacctt caggtcgaac ggccatcatg atgacaagtg tcaagccaaa
    23881 caaaaggaac ctgtacaaga tcgggttcgc attgtctttg actgccagtt gaacaacggg
    23941 atcgctaatt gaaggaagaa ccgtgtgtgt taaaagagag gaaaggaagt ctttcagcgc
    24001 aggcaggaag aggcggtctg cggtcatcaa tacaagacca ccgacaatta cgccgttgat
    24061 attgccgata ccaccgagaa tgaccacgca tagaatgatg atggatgcgc tgaagtcgaa
    24121 aacacttgga aagataccgc ggatataagc tgcatagaat gcgcctgcaa accctgagaa
    24181 ggtggcaccc atggcaaacg cggccagctt ggtgcggacg gggttgatgc ccatttgtgc
    24241 ggctgccaat tcatcttcac gaatggccat ccaagcgcgg ccaaggcggc tgtcgcgcag
    24301 gcggcggatg atgaaaatcg aaagcaggat gatcgcgatg atcaggaaat accaggttac
    24361 cgggttgctc gattcaaatt ctccaataat gggaagtgag ggccttccaa tcggactgat
    24421 gcctttttca ccgagtgtcg catcaaaatg ttctgtgaag acgatacagg ttgtattggc
    24481 tccaaacatg ggaaggatcc agcaggtgag cggttccttg atcgtcacat caatcaggtt
    24541 cttgaaaagc actgggacaa tttcaccgaa ccccaacgtc acgatggcaa gatagtcacc
    24601 tctcaacggt agagttggtg caccaagaat gatgccccac acacttgcca ttgctgcagc
    24661 aatccagatc accagccaga aattccactg aatattgtga acgggtgaag agagaatacc
    24721 tgttgtgtat gcgccaatcg caaagaaagc cgcataacca aggtcaagca ggcctgcata
    24781 ccccaccaca atgttgagac caaggcccag aatcacgaag atcaatgcaa agatgatttg
    24841 tgccgtccag cgcaaagcag tagctttatc cacaaagggg aaaacgatca caagaagtgc
    24901 caaaagaatc atcttggcat attgttcttg tttttcagga agtccgcgca gggaggccag
    24961 cagagtggtg aagatgagcg caccaaagaa gccgatcaga ttggcattca ttgcaatatc
    25021 tgctttggct ggctttttgc cgatcgcttc catgacgaag ttgttttgat acaggcttaa
    25081 tccaacaagc aggactgccg cgaccaatgc cgacgggaga actgccaggg cgattttgag
    25141 tggatccttg cgttcgaagt tattgccaag ccctaatcca aggccaaccc ccatcagtac
    25201 gccaaggact gcgaccgacc atcctccaaa aagaaaggtc agcaagccgc cgatgatgcc
    25261 gaaggtaatt ccaggtcgaa taagtttcat gaatgtgcct cctaggcctt ctgtccggtg
    25321 ttttcaccga gcaagccggt cgggcgaaat agcagaatca agaccagtgt ggcgaacacc
    25381 catgcatttg tccagcgggc gttgagataa ccgtcactga aagaagccaa cagaccaatc
    25441 aagaatccac caagcatggc gccggtgatg ttgccaatac ccccgagcac agcggctgtg
    25501 aaggattgca aaccagcacg gaagcccata aaccaccagg cggtattgtt gtacaagcca
    25561 gccattaaac cagctgcgcc agcaagacca ccaccaatca ggaatgtggc tgtgataacc
    25621 cagtcaatat caataccaag cattttcgcc gcatcgcggt tttgtgcagt ggcacgcatg
    25681 gcctttccaa ggcgggtgcg ctgtacaaac aaatacaggc cagtcatcag gccaacggcc
    25741 agaacgatga ccaacaggtc tttgatcgta aaccgaagtg caagcccact tgaagccaga
    25801 agatttacat caatgccaag gctggtgaat aacgtgccaa gcatatcggg aaatactttt
    25861 tgtgatgcgc caccagtagt accgaaagac ggcaccaacc aggcttgtgc agcccacatc
    25921 aagccaatgt tctcaacgat aaaagacatg ccaatggctg aaatcaatgg cgcaaggcgg
    25981 ggggcgtttc gaagccgttt ataggcgagt ctctcaatag ttgcattcag cgtggcacag
    26041 actacgaccg ccacaacgag tgccagaatt aatcccaaaa taatcttggc aggatcttta
    26101 ctatccagtg tgccggtgac agacaataca gtaagggagg taaaaacacc aatcatgtaa
    26161 acatcgccat gggcaaaatt gatcaactcg atgatgccgt acaccatcgt gtatcccaaa
    26221 gcgataatgg caatgattgc gccgttggcg atgccgataa ttaaaaattg aaccaactgc
    26281 agtgggttgg cttttatggc ttgcacaatt gcaggtacgt caagcccggc gattgggcca
    26341 atcagaaaaa cgatgtaaag aacaattgct attgtgccaa ggcggcgcca gtttattttc
    26401 ttgaggtttt ccatgattct cctctacggt accttcaact tcccttgtac ctgttaagaa
    26461 tagtgggggc ggcaaagatg ccgcccccag aacttgctag agacttattt gaaggtgcca
    26521 acaggagcgt aagcgccgtc tttgacctga tagaaggtca tgtcggtcag gcttgtgtca
    26581 ccgtttgcat cgaacgacca ggtgccgagg gcgccgtcaa agttctgaat ggcgaacacg
    26641 gcggcgttga tggcagcgcg gtcggaggga tcgccaccgg cggcgcagac gtcttcaatc
    26701 gcgcggagcg caacgttcat ggtttcgtag ccgtacacag cgtagggctc ggtcaaagga
    26761 ccaaacttgg cttcgtaatc agccttgaac tgcttaccag cttcggggag ttcttcgaaa
    26821 ggaacaccag cgacggaagc atacacacca gcggccacat cggcgccagc gccatcaatg
    26881 aaggcctggg tctggatacc atcgggacca acgaacttga cggtttcgtt gtcacccatg
    26941 atggcgacct tgtccttcag gagctgggag gcgttgttgt caacgaccat accaacgtaa
    27001 atggcatcag gagcgccacc gttgttgctg gtggagatct tggtcatgag ggccttgtaa
    27061 tcagcagcct tggggtcaat accttcgtga ccaacaacgg tcaaaccgat ttcattggcg
    27121 gtggcttcga acacatcagc aataccctta ccataaagtt cctgatcgtc gaggatgtaa
    27181 acgggggtgg cgccgagaga cttcacgaag ttggcggcaa ctttgccctg aatgtcgtca
    27241 gcagcaacta cgcgggcgta ggagcgggtg ccggtcgggt agtaggaatc ggttacgccg
    27301 gggaggtcct tatcagcctt ggtcaaacca gggttggtgt tggcggggga gatcatgacc
    27361 aacgggccag cagcgttcaa aatcggaatg gacagcttgg cagcgccaga gttgaacgta
    27421 ccgaggtagg cgatgatggc ggggtcagca gcagccttat tggcgttttc ggtctcgaca
    27481 gcgggatccc acttaccgag agcggcggag gcatcatccc aagcttcgta ttcgagggtg
    27541 acattgccac cacaggctga gtagttagcc tgttcgaggc gcaactgcat ggcgttcacg
    27601 atggtctggg tctgggtcag ggaggaacca gtcatcggga ggctggagac gatcttaagg
    27661 gtaacggctt cagcggcagg ggcttcggta gcggcggggg cttcggtagc agcaggggct
    27721 tcggtagcgg cgggctcttc agcgggggcc tcggtagcgg caggagcgcc accaccacaa
    27781 gctgcaagca tcatgctggc aagaaccaac acggaaagta aagcgagcaa acgcttattc
    27841 atttttctta ctcctccaga atatattagg tgaattcgac gtcccgcaat gatgcggggt
    27901 tacacaagca gcggacaatg cttacgatgt cagcctcacc tccttttggg taaagtactt
    27961 cctcttttaa aagaaagccc gaaaaccttt ataacgattt tcgaagccca ctctaaaacg
    28021 gcaagctttt catttttttt attaaacggc aatgataagg gggtttttct ctaaggtcaa
    28081 gagcacgaat tttatttttt atcgtgtggt ttatacaaca attgttcacg gatgcgcaag
    28141 tctgtgttac gaattttcat tgcaaggtca gccgcaagat tgtacatcaa ccgatacccc
    28201 aattgtgggt aggtttcgca aagcataatg agtttgtcac gttgtatgac aagtaaccga
    28261 gtgtcttttt gggccgcccg agcagatgcg gaccgtaatc cttcatccac cagtgcaact
    28321 tcgccaaatg attgacctct gcgaaggcgt gcaacggcag tttccttttt atcagattcc
    28381 cccgtggcac cacggttgat caaaatatcc acctcacctt gtgcaatcac gtacaattcc
    28441 ttgctgccac tattttcttg aaagacgagg tcaccagcat tgaacacaac ttcctgacaa
    28501 aggtttgcca ccagttccaa ttgagtgggt gtgaactggt aaaaaatatc gccttgcttg
    28561 agaaagttga ccatgctatt cataagttga gagtttagca taaataaata tgatgtgcaa
    28621 ctgtttttgc gatgaaatca aaagacgcct ccagtctacg ggtaagtcag aatcaaccaa
    28681 tcaagagggt ccacttggat tccgtttacc cataactccc aatgcagatg cgccccagta
    28741 acgcgccctg ttccgccaac aagcccaatc acttcgttct gttgcaccac ttgaccaacc
    28801 tgtacattga tctgggattg atgccagaag ccgctgtaaa ttccccagcc gtgatcaatg
    28861 atagtggcat ttccgcgtac tgttaacggg ccagcaaaaa caacctgacc agccgccgga
    28921 gcagtgatcg gcagcccatc accaccgccg aaatcaagcc cggtgtgaaa acctgtgatt
    28981 tgcagttctg taccttgccc aatgtaagtg cgacgattgc catatctgga agtgaaataa
    29041 gtggattcgg cgtatgcgat cgcaggagaa ataaaatcac cgctccaata tttgatcgga
    29101 ttggaaggca gagtaagttg aattaattgc tgcaattctg gttcggtgga ggcagggtca
    29161 atggtggcag ggtcaacata tagcagcgga tcatcaggat aattaccgga aatgatcaaa
    29221 atgaactgct caaaagattg tttgctccca tctggcagcg tggcttctaa tcgtaaagga
    29281 tacaccccgg gcggcaacaa ggcatgtacc ccttgaagag ctacttgagt cccatcttcc
    29341 attggaaaaa aatgtagagc gtggtcaaca aggagcccgc tgagtgtcac atttggaatc
    29401 gtttttactt tgataacacc cgtgccgccc tgtttgatcg gtagatcacg aatctctgca
    29461 ctaataaaag cagcaggtaa accgctggtc gtttgttctg cgttatcttc accaacggta
    29521 aatagtgtat cgcctggcat actatcccag gagcctgcca gatggttgtt gtgggaaagg
    29581 gtccatacat ctgtgttttg ttgaacagcc aattccagca gcgattgtcc cttggtggga
    29641 gagatgcgtt tgttcaaacc ctggctccct gattgcacgg ggacgatcat acttacaccc
    29701 acataaaatt cactggggct taccacgtga tttaattttt tgaataaagt ttctgatacc
    29761 tgcgtacgcc tgataaggct gcgataagag tctccaaagt tgacaacttc agtgttcaac
    29821 gtccctgtga tgccacttag cccggggatg ataagcgcat ccccggcgtt gagttgattg
    29881 gcattggcaa ttccattgac tgccatgaga tcggccaaac tgacactgaa ccgttctgca
    29941 atggaggaaa ggctatcacc aggttgaaca atatagatgg gaccgcccgt atcctgggct
    30001 tgtgcaggtt gaaagggaaa aaacaaagat agaatgagaa ttagcaaaat gatattttta
    30061 gacatggttt ttgaaggaaa tactggagtg agtgcagtgg tttattgcag gttcttgaat
    30121 tcgacctgat cgccaatttc gtaatccccg aagcgttgcg ggtgaatttc aagggtgtat
    30181 ctggcatctg cttttggaaa gtaggctgga tgccatgatt tagcgatgat cttatctaca
    30241 actttcattt cagaattgat ccaaaatact gccagatcga aggggacaaa gaacatatgg
    30301 atggaagtgt cgaggcgcga atctcgtttt tcgaccagaa gcaaaccctc gttaagttcg
    30361 aggcgtttac ggaacatgag tccgcgcagg cggcaggaga atgaatcgca atatcccaca
    30421 cgggcgggct gttcgatcat gcgatttaag ttgtgtacaa aaatgggacg gggcatgtgg
    30481 gttatataaa acaggacggc atttccgccg ccctgccaaa agataaaaga atttcaggat
    30541 gatagttttt ggccgacaac tttttcgaca ctgtccagca gttcttgcgg gccgaacggc
    30601 ttggcgatgt agtcatcaac tttggcgatg tgcaagccga ggaccttgtc tatgttttgt
    30661 gctctggctg tgacaacaat gacgggaatt tcacgggtgg atgaatctgc tttcaattgt
    30721 tgatatactt cccacccgtc catttcaggc atcatcaagt ctagcaacac aagatcagga
    30781 tgcaggtcgc gaaccatttt gaggccttct tttccaccag gggcgccatg cacgtcaaac
    30841 ccgcgccgcc cgaggatgag ccgaatgagc tcaatcattt caggttcatc ttcaatacat
    30901 acaatatgtt tgatagatgt attattcatt tttctcctgt ttcttaaaat aagtttacca
    30961 taaaaaaaat atggatttgc cgatgaaaat catcacatgg aacgttaatg gtattcgtgc
    31021 tgcgcttgga aaaaatgcgc tggattgggc ctttgctcaa agccctgatt ttttgtgcct
    31081 gcaggaagta aaagcccgcc aagagcaact ggatgaggaa caactcgcca aattaaaact
    31141 ttcttttgtc tggaatgccg cacagcgacc gggatacagt ggggtaacga cctttttcaa
    31201 aaatcaaccc gatgaagttg taactggcat gggcagggag gtttttgatg tggaaggccg
    31261 tgtcattcaa acccgttggg ctggttgccg tctattcaat atttacttcc cgaatggcac
    31321 ccgtggacat gaccgcgtgg aatataaact tgatttctac gctcatttgc ttgagttgtg
    31381 tgatgccctg catgaaaaag gagagaacct tatcattacc ggtgatttca acaccgcaca
    31441 catgcccatt gatttgaaaa atccgaagca aaacgagaag acttcaggct ttttgcctga
    31501 ggagcgggag tgggtgcaaa aatttttaga tcatggattt gtggatgctt accgcaagtt
    31561 gtatccagag aagatcgaat acacctggtg gacttatcgt ctggatgcac gaaagcgtgg
    31621 tattggctgg cggttggatt acttccttgt ctctgaaaaa ttgatgcctt ttgtaaaaga
    31681 tgtgatcgtc cacgataaag tgatgggttc cgatcattgc ccggtggaat tggttttgga
    31741 gggggagggc ttttaatttc ggtcgaggtt ggtgtcatcc cacgaggcag ggtcgttgag
    31801 tgattcgagg tgggtgaaga ccgtgacatt tgggagggct ttgcgaatgt cagactcgat
    31861 gctttccagc agtcggtgtc cgcgctgaac ggtccatttg cccggaacaa ggatgtgtac
    31921 cgacacgaat ctccgtgcgc ctgattggcg ggtgcgaagg gcgtgatatt ccacattatt
    31981 ttttgtgtat gagctcagga cgttttttac gagtttttgt tcatctggtt cgagagctgt
    32041 atccattaat cccagtgctg acatgcgcac aattcggaat ccagaccaga tgatgttccc
    32101 cgcaaccacg aacgccacga tggggtccag tcgttcccag cctgtcagca ccaccagccc
    32161 aacacctgcc aacacaccga cagaggtcca tacgtctgtc attaaatgat gggcgttggc
    32221 ttcgagtgtg atggagttat gttttttgct ggcctgcagc aaaaccaaag caacaagcag
    32281 attggcaagt gaggcggcta tagaaacacc cagtcccagc ccaacctgtt caagcggttg
    32341 tgggttgata agccttggaa tggctgcaat gatgatgctg gccgccgcga tcagaatcaa
    32401 tgcaccttca accccgctgg agaagtactc cgctttggtg tggccgtaag cgtggtcttc
    32461 gtctgcgggg cgggcagcga tggtgagcat ggcaagtgcc atgacagcac caaccaggtt
    32521 gacgatggat tccaaggcat cggagagcaa gcctacggac ccggttagaa agtaagcgat
    32581 cgtttttagg gtgatcgtaa aaatcgctgc gccaattgaa agccaggcaa atttggtcag
    32641 gaacaggcgg tcgttgggtg atgaatgtgc catgctggtg attctatgtc gaagcctgtt
    32701 ttgcggttag tgacatttat cctccatata tgagtttgtg gaggaattgt gtaggttttt
    32761 cggggaggga gatttttgat tgtttttgca agcagttttc ggctaaaaat gaccgcatta
    32821 tttcagtcaa aaatggccgt attagaatat catataatat ttacccagta cgcgtcatct
    32881 aatggtatgg cttggtataa tctgcccgta cgtcatattt ggaggattcc gtgaattccc
    32941 gtagtcaatc gtttttcgtg tacttccttc tgatcgttgc cattggcgcc atgttttaca
    33001 tgggtatcag cgaaaacttg aatacagaag agccgctgac cattaatgag gtggctctgg
    33061 ctgtgcaaaa gggtgaagtc gcccgcattg tgatcggcag cgacgatacc gttcgtgtga
    33121 ttttcaataa cggtgtggaa gaaggcgttg agtctcgtaa agaaccggat gccacatttg
    33181 tggatcagct tcgtagtctg ggtgttacga cggaacaact tgcccctgaa aatgtagtca
    33241 tcgaagtgag cgctccctcg gtttgggcgg gtgttttaag cggtgccatt tatattttgc
    33301 ctgttatttt gatggcgggt gtgttgtggt ttattttccg ccaggcgcag ggcagcaata
    33361 acgctgccat gtctttcgga aagagccgtg cgcgcatgtt cagcggggag catcctactg
    33421 ttacctttgc tgatgtggcg ggcgctgaag aatcgaaaca ggaacttgaa gagatcgttg
    33481 aattcctcaa ggagccgcaa aagttcattc aactgggcgc gcgcattccg aagggtgttt
    33541 tgctcgttgg ccctccaggc acaggcaaga ccttgcttgc aaaagcagtt tccggcgaag
    33601 cgggtgtgcc gttcttctcc atctctggtt ctgaatttgt ggaaatgttc gtgggtgttg
    33661 gtgccagccg tgttcgtgat cttttcgatc aggccaagcg tcactctccg tgcattatct
    33721 ttatcgatga aattgatgct gtgggtcgtc agcgtggtgc cggccttggc ggttcacacg
    33781 atgaacgcga gcagaccttg aatcagatgc tcgttgagat ggacggcttt ggcactgata
    33841 ccaatgtcat tatcattgcc gccaccaacc gccccgatat tttagaccca gctttgctgc
    33901 gccccggccg ttttgaccgc cgtgtgactc tcgaccgccc cgacgtgaag ggacgcgaag
    33961 ccattctcaa agtgcatgtc aaaggcaagc cgttggatcc gaaggccgac cttgcttcgg
    34021 ttgctcgcgg tacacctggt ttcgtgggtg ccgatcttga aaaccttgtc aatgaaggcg
    34081 ccattttggc tgcgcgccgt aataagaaat ccatcggtca atctgaattg gaagaagcca
    34141 ttgaacgcgt ggtgatggga cccgaacgca agtcccgcct gatctctgat gaagaaaagc
    34201 ggatcattgc ctaccatgaa gctggtcatg ccatcgtggc aaatgccatc gctgaagcag
    34261 accccgtcca aaaagtgacc attgttgggc gcggacaggc tggcggcgtg acctggttcc
    34321 gccctgatga agaccgcatt ctcatgtctc gcaagaagat gctcgccact ttagccatgg
    34381 ccctcggtgg tcgtgctgca gaagagctta tctttgacga tatcacctct ggtgcatcca
    34441 atgacattga gcaggtgacc cgcatggcac gtgccatggt cacccgcctc ggcatgagtg
    34501 ccgaaatggg caccatgacc tacggccaaa aagaggaaat gatcttcctt ggccgcgaaa
    34561 tttccgaaca acgcgattat tctgaagctg tggccgaaca gatcgatcgt gaggttcgca
    34621 agatcgtgga agaatcatat aagattgcca gaaacacggt taagaaatat cgcaaacagc
    34681 ttgatcttgt ggccaaacag ctcctcgaag ttgaatccat tacccgtgaa gatttcgaaa
    34741 agatattccc cgcgcccctc ggcaagaaga gcggcacacc gcaagcggta taaattcttg
    34801 taaagaagca accgataaaa aagaggcttt ccaaaaggaa ggcctctttt tattaccaat
    34861 taccaattac ttactttcca ccctctttgt gctgcctcac caactcacgg cgcaaaatct
    34921 tgccaaccgt tgtctttggc agttcagtgc ggaattcata gtgggtggga accttatacg
    34981 gagcgaggtg ttccttgcag aaggctttga gttcggcttc tgtcaaggtc tcaccgggtt
    35041 tcaccaccac ccaggctttc actgtctctc cacgctgtgg gtcagggatg ccacccacgc
    35101 ctacttccag caccttcggg tgatccatga tcgcttcttc cacttcacgc ggccatacct
    35161 ggaagccgcc cggcttgatg agttccttct tgcggtcaac aatgtagaag taaccatctt
    35221 cgtccatgcg ggcgatatcg cctgtgaaaa gccaggtctt gccatctttc atctgacgca
    35281 ggctgttggc ggtttcggtg ggcatgttgt ggtagccctt catcacctgc ggcccgttga
    35341 cgatgatctc gccgatctca ccctgcggca attcggtttc gccatcatca aggctgatca
    35401 gtttgatatc cacatctggc agcggcatac cgatggagcc ggtcttgttc acgccattga
    35461 gcggattgca gtgcgtggcg gttggcgctt cagagaggcc gtatccttcg aataccttgc
    35521 cgcccgtcag tctttcaaat tcatcttttg tttcgcgcat caacggagcc gaccccgaaa
    35581 tacaggcttt gatggaggaa aggttgtatt tccctgcctt cacatcggga tgattattga
    35641 tgccgttgta cagcaaaggc acaccgggga agatggtggc cttgtatttg ctgatgtttt
    35701 ccagtacatc cttcagatcg cgtgcattcg gcaccatgac catgctggcg ccatttgcca
    35761 tggcaaaatt catccccgcg accatgccat acacatggaa aagcggaatg cccattaaaa
    35821 ccacctcttt gccaggttca agagcgggca tccatgattt gatctggagg gtattggcaa
    35881 ccacattgcg gtgcattgcc acagcaccct ttggcacacc cgtcgtaccg cccgaatact
    35941 ggaagagggc agtgtcatcc ggagttactt ctacgttggg tttcggggag ttcgcatgtt
    36001 tggcaagcag gtctttcatc cacacatccc cgctggccag ggtatccaca cggtcgcctt
    36061 cctttttctc tttgacaagc gtgaaaagca gacgtgtgat cggaggcagg gtttccttga
    36121 tattcgtaac aatgatcttc ttgagtttgg atcgttcacg tgcagtcttc aacttatcgt
    36181 aaaaacggct gagtgtgaac atgatttcaa tattggcatc gttcacaggg gtgatgatct
    36241 catccacagg gtaggtgggg tttaccgcca ccaccgcacc acctgctttt aaaatgccgt
    36301 aatacgcaat gacaaactgc ggcgtatttg gcatgaagat acccacccga tccccttttt
    36361 tcactcccat ttcaaccagt gcggcagcca tgtggtcggt caatgcattc atttcacgat
    36421 acgaaatgac cgcacccttg aagatcgtgc aggcgcggtc aggatattgg cgcgcggctt
    36481 cctctaaaaa atggaaaagc ggcgctttag ggtaatcaat tgtttgtggt acacctttat
    36541 cgtaattcgc gagccacgga cggttgttca tgatttgctc ctccttcaag ggatgtgtga
    36601 agtatataca atcaagaatc aaaagtcaat cggttgtgga tatgtttttc aacttccatc
    36661 accacgccct ctttgactct aaaccacgcg atttctggat cagactcctt gaaccaattt
    36721 gcctgcctgc ggacgaaaac ccgcgtcgct cgcttcatct caactttggc ctgctcggtc
    36781 gtcaattgac cctgcaccac gctgacacat tctcgatacc cgatcgccga catgcttggc
    36841 aacgatgggg cataccctct ttccagcaga ctctgcacct cgtccacgaa cccagccgaa
    36901 aacatggctt cgatgcgctc atccacccgt tgataaagct cctcgcgtgg acgagtcaac
    36961 ccgatggtaa ttaatcggta gggcgcttcc ccctgtccgc gttgttcgga gaatttcctg
    37021 ccagtagtca aaatcacttc cagggcacgg atcgtgcggc gtacattgcg ggcatcaatt
    37081 ttgagtgcgg attctgggtc aagataggct agtttagcgt gtagccactc tgcccctcgt
    37141 tcttccttcg tcttttccaa ctcatcccgc atcttcggat tttcaaccac ttctggtggc
    37201 tgccatccct gcgtgaccgc ccgcacatat tgccccgtgc caccgactaa aaaggggagc
    37261 cgtccgcgtg tgtggatctc tgtaatgatt cgcttggctt cctgctgaaa gactgccaaa
    37321 ctaagagtct catcgggttc aacgatgtca atgagatgat gcggaatgcg attcatttcc
    37381 tcctgcgaag gtttggctgt gccaatgttc atgccgcgat aaaagaggcg cgaatctgcc
    37441 gagacgatct cgccgttcaa tgattccgcg agttggatcg caagttctgt tttaccgacc
    37501 gcggtcgggc caacgatgag gatgagaggt ggtttgttca gtggcattgt tatgaaatga
    37561 ttggaaaaat catggggctt tctggtacac ttttataaag ttgatttggt tcccgagaca
    37621 tatcgggaca aagtgaatgt tgaatagttt ctattttatc tcttgttgaa caaatcacga
    37681 gaccaaaagg tgagcaaatg attaactggc aggaattaac cattgatatg ttcgtcgagc
    37741 aattgcggac ggtttatcaa cgcacatatg gtgatatcaa cagtgatttt ggccgaatcg
    37801 ttgcctggtg cggacgcctt gcgttggaga acattgccaa ttccgatgcg ctctatcacg
    37861 acattgacca taccatcatg gttgcattgg ctgggcaggc cattgtggag gggaaacatc
    37921 tgcgcgaagg cggggtgacc ccgcgtgatt ggatgcactt catgatcgct gtgctttgtc
    37981 acgatatcgg ctatgtgaag ggagtgtgca ataacgacac caaggaccaa ttcaccactg
    38041 gggtggggaa caagatggtc ttcatttctc ccgaaggcac tgatgttgcc ctgaccccat
    38101 atcacgtgga ccgcagcaag cagttcgtca tcgaacgttt tgggacgggg ctgcttcaag
    38161 atatgaccaa atttctcgat gcggaattga tcgcctccta catcgaaatg acccgcttcc
    38221 catcccctcc aggtgaaatg tacaaggaca caaaaggctt gggaggcctt gtccgtgcgg
    38281 cggatttcat tggtcaactc ggtgaccctg attacctgcg aaaatccccc gcgttgtttt
    38341 acgaatttca ggaattgggc gtcaacgaaa aattcggcta caaatcaccc aacgatttgc
    38401 gcaagaatta cgcatctttt tactggaaca gtgtcagccc ctacattcaa gatgccctgc
    38461 actatctccg cctcaccgaa gatggcaaac aatggatcgc caacatgcat tcccacgtct
    38521 tcgatgccga gcatgaccgc ggggcgcggt aattatccaa ataagagcga tacacattta
    38581 caaaaaataa aaggatacgt tttaggcgta tcctttttga attaacttgg tgaggcaatg
    38641 cacgttgcca tttgggctat gcaggcttcg aatactcccg aatatcatcc gtcgcttcgt
    38701 ccagctttgc cacttcttca tcggtcatgc gccagcctgc gccgcccgca ttgctttcag
    38761 cttgttgcag gctcttcgcc ccagggatgg gaagtgcgcc cttgcagatc agccaattca
    38821 aagccacctg tgaaatggtt ttatcgccat gattcaaacc gatctcctgt aacaacttga
    38881 gtacaggcgg gagtttcttg atcaattccg cgtactgtga ggcgcgcaca ccgggcggcg
    38941 gattttctga tgaatatttc ccggtcaata aacctttttc aagcggggaa taggcgatca
    39001 agcggattcc caattcttta caacgggcga gtgtgccgtt cttttccacc tcgcggctca
    39061 acaggctgta atgcacctga ttcgacgcca gcggaattcc atgccgcgcc agtgtggtgt
    39121 aggcgcggat catcctcttt tcaccgaaat tggaaacgcc cactgtgcgg gtcaacttgc
    39181 gctgaacaca ttcgaccatg ccttccatca tcgtttcagg agtcatcaaa ggcgaaggcc
    39241 agtggatctg gtacaggtcc accgattcca gcccgaggcg ctccaggctg cctttcaaag
    39301 cgcggggaat gaatcccttg gtaagccgcc agggcatggg aaagaatttt gtggcaacca
    39361 gcacgggctg gtctgtttct ttcaagaact gtccgagcag ccgctctgaa tatcccgatc
    39421 cataaatctc ggcagtgtca acgaagcgaa ttccgagtgc cagtgaggca tcgaacgatt
    39481 cgcggatttc cttgtcggtg tgtgtctgcc catagcccca gacgattcgg tcaccccact
    39541 gccacgcgcc caaccccatt tcgacggcgt gcaggaacct ggtttcattg ctgatttcgg
    39601 tcacgatgcc tcctgtgaag aacagccccg attttatctt aaaaaacccc gttcaaaaaa
    39661 aatacaggtt acaattcagc ccgcttaaaa aattttagtg gaactgatgg aatgcgagtc
    39721 tctgacctcc cgccttggga tggagcgggg gctggcgtcc cctgagttcc aggaggaatc
    39781 ttggaatgcc caacttgaat gcgcgggaag ttgaatccgc atctgacctc agccgacggc
    39841 tggggctgcc cttttcaaat ttagccttgc tgacacgggc cctgacccat cgctccttcg
    39901 tcaatgaaaa tccgatggca gtggaagata acgaacgcct tgaatttctt ggcgatgccg
    39961 tgctcgattt cattgtagga gcctgggtgt acaaccgttt tcccgaaatg cccgaaggcg
    40021 acctgaccaa ggtgcgttcg gcattggttc gcaatgaaca gctggcgaag ttcgcccgca
    40081 aattggaact ggggaatgcc ttgcggcttg gccgtggtga ggcggcttcc ggcggctcca
    40141 accgcgacaa tttgcttggg tccgctttcg aggcgctggt cggcgcattg tttttagaat
    40201 caggtctcgg cggggtggaa gcgttcgttc atcccatttt ggaagatgcc cgcgaactca
    40261 tcctgaacga gattcacgac cccaaaagca aattgcaaga gtgggcacag gcgcagaaat
    40321 tgggcgcgcc ccggtatcgg gtcatcggca caagcggacc tgaccatttg gtcacatttg
    40381 agatcgcagt ggaaattgca ggcgcggaaa gagggcgcgg cacgggcacc agcaaaagcc
    40441 atgccgaaca cgccgccgcg caggatgcgt tgaatcagtt gaatgtgctg taaaaatgtt
    40501 gactggtttg caggttgacc tttcaaatgt aaacttgaaa aatccaaaat ctgaaatcgt
    40561 aaatcgaaaa taaaacatgc ctcttcgtct aaaatcgctt gaactgcaag gatacaaaac
    40621 tttcgcatcg cggaccgtgt ttgagttcgc gagcggaatc actgccatcg tcgggccgaa
    40681 tggttcgggg aagtcgaaca tagccgactc gctgcgctgg gtgttgggcg agcaatcgta
    40741 cacactgctg cgcggcaaga aaaccgaaga tatgatcttc tcggggtcgg aacatcgcgc
    40801 acgcgcaggc atggcgcagg ccacgatcac tttcgataac accgagcaat ggctgcccgt
    40861 ggatttttcg gaggtggcac tttcacgtgc cgcacatcgt gatgggcaca acgattattt
    40921 gatcaacggc cagcatgtgc gcttgcgtga gatgaatgaa ttgctggcgc aggcaggttt
    40981 gagcgaacgg acttatacca tcctcgggca ggggttggtg gatgcttcac tggctttgaa
    41041 agccgatgat cgccgccgcc tgtttgaaga agcggcaggt gtggggttgt accgcgcccg
    41101 ccgtgatgaa gccctgaaac gccttgaaaa taccgagcgt aatcttgaac gtgtacttga
    41161 tatcatggcc gagcttgagc cgcgactccg cagtttggag cggcaggcca aacgcgccat
    41221 tgattttgcg cgcgcgcagg cagatatgaa gttgattttg cgcgagtggt acggtttcca
    41281 ctggcaccgc gctcaacgtg acctgaccga tgcacgcgaa accgtgcgcg gacaggaagt
    41341 acgcgtgcgt gacctgcgtg ccgttcacga gaaagcacag gccgaataca cctccttccg
    41401 cgaaagactt tcgggtctgc gtgctcaatt gaatggctgg catcgccaat cggcagattt
    41461 gcacaaccag cgtgaagcca tcagccgcga attggcagtt ctcgaagaac gccgccgcgc
    41521 actgaccaat acccaggcct ccattctttc ggatcaggaa catgcgatgg atgaactgca
    41581 ccttgccaat gaccgttttg gcgaagcgga gcaggaactc ctccgtgtgc agggcgagta
    41641 tgaagaggcc aaacgtcagc ttgaaacctg caaccttcaa ctcgcaaatc gaaacgcgga
    41701 gcgagccacg ttggaggaac aggctcaact tttacgcaac cagatcgaag cctttagcca
    41761 gcaacgtgcc gagaacaacg cacgtctgga tgaattgaaa tcccgcctcg aagcgcaatc
    41821 acagcgcatt gaacaaacca aacaggccat caccagcggc gagtcgctgg ctgcgaaggc
    41881 cagatcgcaa tatgaatcgg cgcacaaagc ccgcgaacag gcggcattgg cattgcagga
    41941 agccgaagaa aaagtcatca gcagacgcaa tgaagtggaa cgcctggaac gtgagcgccg
    42001 cgatgcgctc gaaaaacgaa ccatcgaaga atcggatcat tcgcgtttga aggcacagct
    42061 tgaggtgttg cagcaatcgg agcaatcgct ggctggttat gccgaaggcg ctcgttattt
    42121 gcttgatgcc gcccgccgcg atccacagcg tctgacaggg gtgcggggtg cgctcagcgc
    42181 cgcgttggat gtccctgccg agcttgaaac cgccatcgcc gccgctctgg gtgatacgct
    42241 cgatgcagtc ttgctggatg aaaatcaact cgaagaagcg ttgggtctgc ttgaatccga
    42301 tgaggcaggc cgtgcggctt tgctgccgct tgtaatgcaa gaaaatatct tgcaccaccc
    42361 ttccgatgaa aattgccttg gcattgcttc tgaacttgtc aacgcgccag aggaaatgcg
    42421 cggcgccgta cggttggcgc tgggccagac cttgatcgtc cgaaaccgaa ataccgcccg
    42481 cattctggtg aaggatttgc cgactcacgc gcgggttgtg acactgcgtg gagaggtctt
    42541 ccgcggtgac ggtttgatca tcgctggaaa aaccgcttcc agttcgaccc ttggcaggtc
    42601 acgccaaaaa cgggaatttg aatccgcctt gagcggactc gtcgtccgtt tggctgaatc
    42661 caatgacctt gtggaacgtt tgtcgaatca actggcgggc gcacagcgtg agttggcaca
    42721 ggccgatacc gatgcacgcg aatcccgcgt cagactcggt gaaattcaag agaccgagca
    42781 gcaggccggg ttggagtctg aatcgacgag tcgtcagctt gaatggcaga aaaagcaatt
    42841 ggaacagttg gaagccgagg cgtgggaatc tgcttcgacc cagcaaaaga tcactgaatc
    42901 tcaggctgag gtggaacagc gttcgaccga gacgctttca caattaaaga atatcaatgc
    42961 acggttgaat gaaatggccg tggatgaatt acaagatcag gtttcgtatt ggggcacacg
    43021 cgtggcggtt gccgagcaaa gtgttggcag cgccgccgcc aaacgcgatg agcgcaccaa
    43081 tgagatcacg cggctcgatt cacggcgcgt ggaattgatc tctcgtttgc aggaagccga
    43141 aggcttgctc aataatctgg ataatgaaaa ggcgcgttta cgtgagaacg agtcgggtct
    43201 gcacgggcag attgacgaga tgcgggtttt gattgagccc gccgaaaaag atctggaaat
    43261 ggccgagcag gaggaagccc gtctgcagga agccgaggcc gccgctcagc gtgcttttgc
    43321 caatgccgaa cgctcatatg ggcaggtgca gttggaacag gtgcgcaagc aggaggccct
    43381 cgataatttg caccagaaga tcaccgatga ttttggcctg gtgatgttcg attatgcggc
    43441 ggatgtttct ggccccgtgc cgcttccgct ggacggcatg gtggagcaat tgcctgttgt
    43501 aaccgaactc gcgcccgaga tcgaagagca gttgacccat caccgcgccc agttgcgccg
    43561 catgggtccc atcaaccctg atgcgaagac cgagtacgat caggaaagcg aacgctacac
    43621 cttcatgaag gcacaggtgg aagacctgcg caaagccgaa gctgacttga agcaagtggt
    43681 cgctgaactg gatgaaatca ccaaaaatga atttgtgcgc accttcgatg ccgtggataa
    43741 gcaattccgc gaaacctttg tgcgtttgtt cggcggtggt tctgcacgtt tggctttgac
    43801 cgaccccgaa aacctggtgg atacgggtat cgaaattgag gcgcgtctgc ctggccgccg
    43861 tgagcaaggc ttggctttgc tttcaggtgg agagcgcagt ctcaccgcca ttgcgttggt
    43921 ctttgccttg ctcaaggttt cgccgacccc cgtctgtgta atggatgaag tggatgcgat
    43981 gcttgatgaa gccaacgtgg ggcgtttccg tgacctgttg atcgacctga gcaaggatac
    44041 acagttcatc gtcattaccc ataaccgtaa tacggttcaa gcagctgatg tcatttacgg
    44101 tgtgaccatg ggccgcgact cagccagcca gatcatcagt ctgcgcctcg atcaggttac
    44161 ggatgatatg ttgaagcggg ggtaaacaaa aggatgaaag ataaaggatg aaggatgaaa
    44221 aagcccgcct cattttgagg cgggcttttt ttgtatcagt tttttgaaac gcatatttgc
    44281 tcgtttttac tgcgaagaag cggtaagttt tatggctttt ctttgcaagc tttgcgattc
    44341 aaatttgtta attacggggc agcggttgca tccacagcgg atttggtctc agctaccgcg
    44401 gtgtcttgag cgttggcggc ttcagtggcg atggtggaga aattcggttc gctgggaaca
    44461 cgttcctgcc agaagtcgaa gatttccacg ccgtattctt cgcgggcagc ggtcagccat
    44521 tcctggaaga ccttgtcacg ggcggcgttg tactgatctg cggtgagcgg gcgttcctgc
    44581 ttcgcgatca attggatgat gtggaaacca tactggcttt gaacaggggt ggtggtgaaa
    44641 tcgccggggt tttcgagggc gaaagcggcg gtttcaaatt caggaaccat ggcgcctgtg
    44701 ccgaaccagc caaggtcacc gcccaaagca ccggagccgg tgtctgtgga aaattcctgg
    44761 gccagggcag cgaaatcttc gcctgctttg atcttttcga tgagattgag cgcggtggct
    44821 tcatcttcaa cgagaatgtg gcgggcccaa acctgctctt ccgtgtgggc tacgtcagcg
    44881 gtgatgatgt ccttgagttt ggtttcgaag agctggtttt cgaagaccag tttgtacaca
    44941 ttgtcgtcga agcccagttc catcaggcgc tcgttggcgt cattgaactc ggtctggaaa
    45001 ccttctgccg tgtagggggt ggctgtcggc tccggagtgt tggtcggacc aagtgtcggg
    45061 gtgacagtga gcgtggcggt gggggtgaag gtggcggttg ggacaagagt cgctgtggct
    45121 tccggggtgc cggtgatcgt cgcagggtca atggttgcgg cgggggcact ggtggcggtg
    45181 ccagtcggct ctgaggtgtt ttccggcgtt gcgctgggca gcggggtgat ggtcacgata
    45241 tcgaaggcgc cagcaggcgg ttcgggcatg atcacttcag taggagtgac agatggagtg
    45301 ggcgacccgt tcgggaaata tccgaacgcg ccttctgtca actcactgat ttcctcttca
    45361 ctcaccgtga ttccgcgttt ggcggcttcc tggcgaatga tctcttcatt gatcatctga
    45421 tcaagcaccg ctcgaccgat ggaggtgggg gaatccagag aagcctggat ctgctgtaac
    45481 tgtgagctta catcaagacc gaactgcgca tactgtgagt aggtctggta ttgggccagt
    45541 aattgctggc gttgtaaacg aacgcgcgct tcaaactgtt ttgcgaggat ctctgtttca
    45601 ccaaccttgg cgaccgggcg cttgagctgg aaataattaa tatccaaata gccgtacaaa
    45661 accaaaagca gaaccgcggc caggatccca ataaaggtat ataaaatgat ctggctttgc
    45721 tgcttttcac gttgaaggcg tgcaacgtgt tttttatgca aaacgggatt ctcgttaggc
    45781 atcttcttgc tcctttatga tggttgcata ttgcttttca atgggtgtca ctcgcagatt
    45841 catcctaccg ccctgagggc gacatgcctg acctcgcccg gtcattccat ttcggtttgt
    45901 attattcggg gaagggtggg cgctgaaaat ctccggcgca atgacaaccc acggctaatc
    45961 acacctgctg tgcgatcagc aaagggtcgg cataaattcg gggcatggga atcgctccca
    46021 taccccgcga aattcttaaa aaactatgaa cggttgtcgt ccagggcgcg gccgctgaag
    46081 ggcaaaaatg ccacgtggcg ggcctgcttg acggcggcag ccacaacgcg ctggtgcttg
    46141 gcgcaggcgc cagtctggcg gcgcgggcgg atcttgcctt cttcggttat gtatttgcgc
    46201 agcagatcgg tcttcttgta atcaatgacc agcatcttgt cggcgcaaaa ttggcagaac
    46261 ttgggcttgg cgaaaaatct gcggtcaccg ccgccttcgc cgccgcccga atagttgcgt
    46321 tcttcactca tgatttactc caaacaaact aaaagggaaa ttcatcctcc gccatggaag
    46381 agccattgtc ggcggaagaa agatcggcat cctgtgaatg gttgttgttt gcatcgcggc
    46441 gatcaccaag catcatcatc tcgttcgcaa cgatctccac gctggtatgc ttttgacctt
    46501 ccttgtcatc ccagcggcgg gtttgcaaac gaccctcaac aaaaacctgc tgtcctttta
    46561 caagatactg tttgcagatt tcagcaaggt tgccccaggc caccacgtta aaccactcgg
    46621 tctcactgtg gcgctccccg tccgcgctgt tccacgaacg gctcacagcc accgaaaaag
    46681 tggtgacggg ttttccagat ggtgtataac gcatctcggg gtctttccca agatgtccga
    46741 tgatctgaac tttattaagg cctcggctca tgataattct ccttgataaa attaccgggc
    46801 aatgcaatat ggaaactaac caacaacaga aagcatgtga cgaatgatcg tctcttggta
    46861 gcgcaggttg cgctcaagat cagaagtcgc agatggattc atagaaacgt tcaaaagaac
    46921 gaactgacct tcgcggtgct tcttgatgat gtaggccagc ttgcggcggc cccaaacatc
    46981 aaccttatca acactgccgc ctgagtcact gatccaaccc ttcaccttat cgagcacgcc
    47041 gttaaaagca ttctcgtcca ggtctggctg catcacacag actaattcat aattacgcat
    47101 agggaaaacc tcctttccat gggtttgttc ctgttcaaac cgttggttag ccaggaacgg
    47161 aaaggcacgt atttggatga cgtacctaat ttagcgggcg aagtttacca caaatttacc
    47221 gctttggatg aaaccccgct tcgcacctaa ctgaaaactg ctcccgtccg aaaggcattt
    47281 tcagcatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntac ctcacataga
    47401 tcggattact gtagatccac ccacgcagtt tgcccaaata cctgcgatac acctcaattc
    47461 gatagacccc tgcatcagta gtgatatggg tgcaggacga tttattcttc cacgtttgaa
    47521 tcacaacccc gtccttcaat aaccgaatct ctgcatctga cggcagttta gcctgcaagg
    47581 tgacacctcc cttggcggga atctcatcgc ccatgatcgc cgttttctca agtccctgcg
    47641 ccgtaaagcg gaatccgcgg gtgggggcgg gcatgtcata ccctacaaag cagtgacctg
    47701 ccgccatcgc ctcgtagatc agttttttat cgttgttcac atcgccgctc aagccctcgg
    47761 ggaggatcaa gtgcgtgttg accgtacgga agtgaaactc ataggggaag atcacgcggc
    47821 tgatcggtcc catgctcatc cgcaaagcat gggcgtctga accgccgatg gcaaccacgc
    47881 gtgcccctgt tgccagtaat tcgtcccatt tcaagatcgt attggggatg ggttggtgcg
    47941 ctacgaaaaa agggaaaaaa gcataaaatg caccatgtaa ttttgtggga actacggtct
    48001 tcaactcgct taacgcattc caaagttcga tgccagtgta attccgtacc gaccaatctt
    48061 cccaggaaat atccgtctcc ttaaaagcgg gtgcggcagg gtcatctggg tgggcgatga
    48121 aggccagccc gccagaagcc tgcacctggt caatgagcag ctgcgggtca tgggcgaacg
    48181 tagcgagttc tcttccgctg ccaaagacca gcaggtgatt tttctgcggg tcccgcgcct
    48241 gatcgtgaac ttcctcaccg atcaacatga ggatcttttt atttttttcc ttgtaatagc
    48301 cttcaaaacc ctgaaccaat acattgtgat cggttacgat cacggcatcc acacctgccg
    48361 caagagcagc tttggcgatg tcctgatgcg ttccgcttcc atcagagtag cgtgtatgca
    48421 tatgcaaatt gacaatgact tcagacatac attcctctat atcaaattct ttcctcttcc
    48481 ttcattaaat acgtaggaag aggaaagaca attcaagaaa acctttgttg acgattcact
    48541 tctgaaacag gtataatctg cccgctaaat ttaaggaggc acatcgtatg gaaaagtttt
    48601 tggatattgc gctgattata acctcagtgg gtttgatcct tggcgtcatc ctgcagagca
    48661 agggcgcagg gttgggcggc ttgaccggcg ctgataccgg tggcgtattt accgcccgcc
    48721 gtggcatcga gcgcatcctc ttttgggtga caatcggatt ggctgtgata ttttttggtc
    48781 tcgtcttcgc agtgatcctt attggaggct aaaaaccgca agcctttgaa gggccgccgg
    48841 gccattgccc ccgtgtgggg cggcggcggc ctttcttctt tttacaacaa attatgaaaa
    48901 aactacgctg gcagattctg gttgtagcgg tcacggtggt gatcgtcgct ttgctcttgc
    48961 tcagccaaca accaattagc acgtccttct tgcccgaagc cgcaccgggg ggaatctata
    49021 ccgaggcgct ggtgggctcc atgggacgtc tcaatccaat gctggattgg aacaaccctg
    49081 ctgaccggga tatcaaccgc ctgatcttta gcgggttgat ccgttttgac tcgcatgggc
    49141 tgccccagcc tgatctggct gaagcatggg gcacttctcc cgatggcaca ctctacaatt
    49201 tctcgatccg ttcgaatgcg gtctggcatg atggtcagcc cgtcaccagt gacgatgtca
    49261 tcttcaccat tgagatgatc aaaagcagcg gctcactctt cccgcaggat atcaaagacc
    49321 tgtggtcgca gatcgagatc aagcggtttg acgataaaac cttccagttc aaacttcctg
    49381 aaccgtttgc accatttttg gattatgtaa catttggcgt tctgccaaaa cacctactcg
    49441 aaactgttcc cgcagaccag cttgccacag ctcaatttaa cttgcagccc attggcaccg
    49501 gcccgtataa gtttgagcgc ctgttgacca gcggcggaca gatcgtgggc gttgcattgg
    49561 ccgtgaacaa ggaatattat ctgccaagtg ctttcattga gcagatcgtc ttccgctttt
    49621 accctgactc agcttcagca cttgatgcct accagcaagg ggatgtcctt ggggtcagtc
    49681 agatcaccaa tgatgtgctg cagcaagctc tgatggagcc tacactctcc gtatacacca
    49741 gccgccttcc gcaattggga ctggtctttc ttaatctaaa caatcccacc gtggcctttt
    49801 tgcaaagcga caaggtccga catgcgttgt tgcttggcac caatcgcgag ttgatcgttt
    49861 cgcgcatcat gcagggacaa gccatcgttg cagatggtcc gatcctgccg gggtcatggg
    49921 ctcattatga tgagatcgag cattttgagt acgaccccga tgctgctgcg gcgctgttaa
    49981 aagctgaagg gtatgttatc ccagccagcg gtggagatgt acgcgccaaa gacggtcagt
    50041 acctgaactt tacgctgacc catcccgatg acgagatcca cacccgcatc gcgcaagcca
    50101 tccaatcgga ttgggctttg atcggtgtac ggctggattt gcagccagtc tcctatgact
    50161 cgcttgtcaa tgattatttg accacgcgaa attatcaggc ggctttggct gatctgaata
    50221 cagcccgcac accagaccca gacccatatt tgttctggca tcaatccgag gccactggcg
    50281 ggcaaaacta ttcccaatgg gataaccgta ccgccagcga atttttggaa tcagcccgta
    50341 cagaagcaga tttctcttca agagcacggt tgtatcgcaa ctttcaggtt gtgtttacca
    50401 aagatatgcc atcactgcct ttgtattacc ccgtctattc ctacggtgtg gatgcacagg
    50461 tacagggtgt gcaggttgca ccgatgtatg atgtcagcga ccgccttgct ttggttgccg
    50521 aatggtatct tgtaacccgc cgtgccctcg aagaacaaac cccggtgccg actgcggcac
    50581 cgtagaaaat gactgaagtc ttttctacct tgataaggag tgacaatgtc tgacgcaaaa
    50641 aaggcaatcg aatacgccca tcagaatcgc gagcgtttca cccgcgagtt gaccgaattc
    50701 atcaaaatcg aatcgatctc caccgatgat gcctacaaag cccaggtgct gcaggctgct
    50761 gagtgggcgg ctggccatct gcgcagtatc gggatcgaga atgtaaagct catgcccacc
    50821 ggcgggcacc ccatcgtgta tggcgattgg atcaagaacc ccggcgcgcc gaccgttttg
    50881 atctacggtc attacgacgt tcaaccggtt gatcccatcg aactttggga gacctctcca
    50941 ttcgaagcga ccgtgaaagg tgattatctt ttcgggcgtg gtacttccga tatgaaggga
    51001 caggtaatgg cgactttgaa tgccgttgag gccatcatga agacgggcga tttccccgtc
    51061 aacctcaagt tcatgctgga aggggaggag gaaattggct caatgaatat gggcaatttc
    51121 ctgccgcaac ataaagattt gttcaaggcc gatttttgtt tgaaccccga tgcgggtgcg
    51181 atctctgctg atgagccgac catcactttc ggcctgcgcg gactcgcgta ttttgaagtc
    51241 cacgtgtatg ggccgacgcg tgatttgcat tcaggtttgt tcggcggcac ggttcataac
    51301 cctgcacagg cattggccga attgatcgca gggatgcacg atgccaacgg cacgattaca
    51361 ttgccaggat tttacgattc ggtccgccct gttaccgatc aggaacgtga agccacagcc
    51421 aagctgccca ccacaccgga aagttatttg cagcaaactg aagtgccagc cttgtggggc
    51481 gagaaggaat acacgcctgc cttgcgcacc ggtgcgcgcc ccacgctgga agtgaacggt
    51541 ctgctctcag ggtggactgg tcccggctcg aagacagtgt tacctgcgaa ggcaatggct
    51601 aagatctcct gccgccttgt gcccgatcaa gaccctgctg aagttcacaa gcagcttgtc
    51661 cgctacatgg aagaacatgc ccccaagacc attacttggg aagtgaagat tctgcacgat
    51721 agtccctggg caattactga cgttaataac aaaggcgtcc gcgccatggc agctgcaatg
    51781 gaaaccgtgt ggggcaggcc tcccttgtat cgccgtgaag gcggctcaat tggtgtggtt
    51841 gccatgctgc aaaatatctg cggagttgaa tcggggcttg tcggtggcgg cctgcccgat
    51901 gacaatattc actccccgaa cgagcgtatg catttgccaa cctggcacaa gaacatggat
    51961 gcattcatcc atttcttctt caatatgaaa tagaaataaa aaattgacag tcacccccag
    52021 ggtgactgtc aattcataag gattatttat ggaagacaga attatcacct atggcggaca
    52081 ggccgttctc gaaggcgttt taatgcgtgg acaaaaagca tttgccattg ccatgcgcgc
    52141 tcctgatggc tccatcacca ttcataccga aaaacttgca aaagtgtacc gctcagggat
    52201 caccaaagtc ccgttcattc gcggggtcat tttgctgtgg gatgccctcg gattgggcat
    52261 gaaagccctc accatttccg cgaataccca aacaggtgag gatgaaaaac tggaaggccc
    52321 cgctctttat ctgaccctgg ccctgtccat gaccattggc attggtctct tctttttgct
    52381 tcccgctggc atcggcggcg cagcggaaca ttatctcggt tggagttcct ggttgaacaa
    52441 cctgaccgag ggtcttgtgc gcttagggtt gctggttggc tatgtttggg cgatcagctt
    52501 catgcctgat gtaaagcggc tcttcatgta tcatggtgca gaacacaaaa ccatcaacgc
    52561 ctatgaagca ggtgcggaat tgacccccga agttgttgca caatacccca ttgagcatgc
    52621 ccgttgcggt acagcttttc tgcttacatt ggttttgcta tccattttgg ttttcacctt
    52681 gcttggccca ttgcctttgc tttggcggct tgcttcccgt attttgttca ttcccatcct
    52741 tgcaggcatc gccatcgaat acatccgctg gacggcgaat catttggaca gccccttcgt
    52801 tcagtttttg atcaagccca acctcgctct gcagcacctc accacccgca cccccgacct
    52861 tgccatgctc gaagtggcca ttcagtcatt tcagtccatg cgcaaagcgg agagtgaagn
    52921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    52981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna caatgcggat cggggtgccg
    53041 aggaagctgt gctctgcgcg gatctggttt tcaagatagc ggatataagt gaagtgcatc
    53101 agcttcggct cgttgacata gatcaggaag gtcggcgggt cggagcggac ttgtgtaccg
    53161 tagtacatct ttagggcgcg gcctgcatga gttggatgcg gatgcgcatc ctgggctttg
    53221 tggatgatcc cgttaatggt ggatgtggtc agacgggcaa ggcgttcctc ttgcacttgc
    53281 aatgccaacg ggagaacctg ctccattcgc tgaccagttt tcgcggagat gaagagaatc
    53341 ggtacgtaat ccatgaagtt caattcgtgg cgaattttgt tcgtgtactc ttccatcgtc
    53401 tcgttatttt tttcgatggc gtcccatttg ttgacgagca ccacacatga tttccattca
    53461 tcgaggatgt aacctgcgat gtgggtatcc tgcgcggtga tgcctgtggt ggcgtcaatc
    53521 atcagcaagg ccacgtcagc gcgttcaatg gacttgaagg cacgcagcac actgtattgc
    53581 tccacgccgt gttcaatcct gccgcggcgg cgaatgcctg cggtgtcgat caaggtaatg
    53641 ggcaggcctt catattccat tttggtgtca atcgaatcac gggtggtgcc tgcaatcggg
    53701 ctgacaatga cgcggtcttt gccggtcagt ttattgagca ggctggattt gcccgcattc
    53761 ggtttgccga caatggcgat cttgatgctg tcatcttctt cttcattttc gggcgccttg
    53821 aaagatgata cgagtgcatc gagcaggtcg cctgtacttg tgccgtgaat ggccgaaatg
    53881 gcaaatggat cgcccagccc gaattcgtaa aattcactgg ccgagtcgcg ccgttcctgt
    53941 gattcgcatt tgttgaccac caaaaaaatc ggcggccaga aggtgccatc gggtaatttc
    54001 ttttgcgagc ggcgcaggat gttcgccact tcgcggtcgg gttcggtgac gcctgtttca
    54061 ccatcgttga tgaagaggac tgcatcggct tcctgaatgg caacctgggc ctgcctgcgg
    54121 atatcttcaa tgaattcgac cgagccgacc gaaagcggag tctttccgcc gtgggttggg
    54181 tcgataccgc ctgtatccac aatgctgaag gtgcggccat tccattccgc ttcaccaaat
    54241 aaacggtcac gggtggtacc gggggtgtca tccacaatgg ctaaacgttc gccagcgagg
    54301 cggttgaaaa gggttgattt accgacattg ggtcggccga cgagtgctac gacaggtttc
    54361 atttttattt tccaatttta aggtgtcggc gttaccacag gtgtgccgat cagagagatc
    54421 acaacttcaa gtgtgttggg caggatcgat tctacaacaa tatctgagat caaaacttcg
    54481 actttcggaa tgacctgatg cgtaccgatt ccgagccccg tcaggtcaac ggtcactctt
    54541 accccttgcc tggtcaatgt gtcgaggagc gggagcggcc ccgaaagaat gacatccacc
    54601 gtcatgggtg agacttgtgc cgtcagcccg gggtcaatgc caatgatctc cactttttca
    54661 ctgccaagcg taacactgct ttcaatgggt gagatgcccg cttgaatcaa aacggtttgc
    54721 tcgccaacaa tggagatgcc ctcgggcaga ttcaatgcga ggcgggtgtt gatgttttcc
    54781 tgcgcattct gcaaactcaa cggctgggtt tccacaatgc caggcaggtt gttgaccagt
    54841 tgcgggtctg atgcgaatac ggtgacaatg ggcggataca cagaaatatc tgtcagacga
    54901 tatccacttg cggcacgccc ggagacgatc acttttactg ccacgtcacg atacccaccc
    54961 tgctgactga ctggcaggct gatgtgaatg gcttcaggtg atacggtaac accttctaca
    55021 gtttgacctt tttcgtcgag gacttcgacg ggaagggtct ggtcaaaatc ttcgcgcagg
    55081 ccgctgacat tgacctgcac acgtgcccgt gccacttttt ccacttgtga ctgtgcgcct
    55141 gcaaccacaa cttcggtcgt ttccagaact ggctcgccga tctggtatcc gatcgcggct
    55201 tcgccggaaa ggctcagatt caggtcgaaa gtttgggtat cgagcgattc cagcaaaaag
    55261 gtcacagtac gcggagaggc ggagacgatc tgtaccgggc gggcatcaat ttgaatctgc
    55321 acttcgctgg tgtgctcacc tgaactcaaa cccgaaaggt cgagcacggc acggatgctt
    55381 tgcggatcgc tttcgatcac atcccatacc gagcgtgggg cgcgcagggt caattccatt
    55441 tctgttggta actcactgcc caaaacgaga tttgggtttt gccctaccac ctgaatcggc
    55501 accgcagaag gaagcgtgcg ggtttcatcg gggtcggctg aggtgacggc agagatccag
    55561 accgccattg ccagcgcaaa tgaccaaaga aatgtgcggt aattttcaga gatccatcga
    55621 aacatgtgta actcaattta gacgaacaac ccacagggtt ttctgttcaa acctgtcaag
    55681 ctgtggggga tgggcgggca atcctatcac ggtttgtctt ttttggtggg aatcaatccg
    55741 gggaatagcc gttcaagtag atttggttta tggtctttgc cattggggcg aaagaaagca
    55801 gtgaggatat tttcgagacg gtcggggtca agccgccgca acattctgcc tgcatgggca
    55861 atggagatgg ccccagtctc ttcggagact acgaccgcaa tggcatcgct ggtttcggag
    55921 gtgccaagcg cggcgcggtg acgcaatccc atttgacgtt cgggcgtgcg gttcagaatt
    55981 ccgctggcag agaggggaag tacgcaagcc gctgccacga tctgggaacc aacaatgata
    56041 acgccgccat catgcagggg ggtgtttggg tagaagatct gaagcagtag ttccggggtg
    56101 acacgggcat acatttgcac gccgctttgc gccagttcat caagggtatc cagcctttgc
    56161 atgatgatca atgcgccgtg ttgacgggcc gataatctgg cggcggcact gaccaccgca
    56221 cggatggtct gttcgagggc cacatctgtt gatttgacag aggcaggcca aatggtcccg
    56281 gcgcgaccga ggcgttccaa tgtccgtcga atctcggggg cgaagatcac tggcagggct
    56341 agaagcaggg caggcagcga attctgcgca aaccatgaaa atgcaggcag gtctaccagg
    56401 ctggtgagca aaatgagggc caccacaagc aggatcatgc cgcgcagcaa tgccatggct
    56461 tgtgtatcgc gcaatgaata cagcaagcca aaaaatatca gggtgacgag catgatgtct
    56521 aatacgctca gccagctgat tctttgaaaa atgaagaaga cgttgttgag gatttcagtc
    56581 acggtaatgg gtgatgaata caggtgtcag ggaaggagat atcctatgat acctgatacc
    56641 tgccgcccag ccctgtgatg gttgcataaa ttccgaactt caggcgagag atagccgggg
    56701 aatcgggatt ccgatggtgg aattatgcga tcgttttaac cctaaatggg gcgcaagaca
    56761 cggtcttgac gcagttgttt gaacagtaat gcaccgcctt ctgtaagggc ttcactggct
    56821 gaatcctgcc aggcttgaac gcccagtgtt tcgggggtgc gtttttcata cccaagttgt
    56881 ttccagagtt cttcctgggc ggcgagttcg ttgaggggga agatgaggga ggcctcgctt
    56941 tgtaattcgc ttgaggcgcg ctccacttcc ttgatcagta tttcgagtgc tttttgcggg
    57001 ttgacgtgct cttcaaggaa aatatcggtg gtgcgggtga ccaggttttc aacctgccaa
    57061 cctgcaatgc caaccatttt tccatcgagc aataaaagca tgtaggcttt ttcgccgaag
    57121 tcttccatta catccaccac ggtgacggtg cgtttgcctt tgctcaagcg ggtgatcaac
    57181 tcggcgatgg caggtgagtg tttgggcttg ccgcgttgaa ccgtgaacgt accgttggag
    57241 gtgacgggaa ccttcgagag cgtttgctcg gtatccgcgg gttttgccac gctggctggt
    57301 acgatttcct tccatgctgt gctgacttgt ttccaaaggt catcgaagga gccggtattg
    57361 gtaatgacaa tattcgccac ggctactttg gcgctttgag ccgattgcat gtgaatgcgt
    57421 tccctggcct ggtcgcgatt catgccgcgt ttgcgaatca atctttcgat ctggatatcc
    57481 tctgtggcat tggtcaccca aatggaatca cataccttac gcagatcgcc ttcgagcaac
    57541 ttgatcgctt caatgacaac gacattttgt gtggagcgct taatgagcat ctctgtggcc
    57601 tgacggacga gtggatgaac gatcccttcc agttgggcca acgcctcggc atcattgaag
    57661 accaggttgc caagtttgct gcggtctatc tcgccgtctt tgttgagcag ccacttaccg
    57721 aacctgtcaa tgacttgctg gtagcctgga gcgcctttgg catacgcacg gtgcgagagc
    57781 gcatctgcat ccacagtgta ggcgccgaga tgctcaagca tttttcgcac aacacttttg
    57841 ccggttgcga tgttgccagt tagaccaatg acgaatttac caggccagtt gctcacagct
    57901 tttccttaat taaagaattt tgtaatccaa ccctatttta atatcttttc aggaaatgtc
    57961 cacctttatg aaaattgcca acatatgaca agtttttccc aggttgactc tcaatcttcg
    58021 agcgtgtatc atcgaatgac ctcaggttga tcgggcaatc gcggtcctgc gttgacggga
    58081 tcgaggaaag tccgcactcc atagagcacg acgtcggata gcacccggga cggggaaccg
    58141 ccgagaggcg tgaacctgcc ggaaaagggc cacagaaaca aacagccgcc ccgcccgtaa
    58201 gggcgggcgg tgatggtgaa aaggtggtgt aagagaccac cggcgtttgc agtaatgcag
    58261 gcggccaggt aacccccgtc gggagcaagg ccaagcaggg gcgaaatcgt ctgtggacga
    58321 tgggagactg ctcgtctccg catgtactcc ccccaaccgt ggagggagtc agccccgggt
    58381 aggctgcacc gagcagagcg gcgacgctct gcccagagag atgattgcac agatggtgca
    58441 aaccattgga cagaatgcgg cttatagatc ggcctgaggt gttttttcaa tctgggcggg
    58501 ttgatccccg ccatttttta ttattcaagg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    58561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    58621 nnnnnnnnnn atggatgaaa atttctattt ccactggaga ctttgtgcat attaaaggta
    58681 aggttgtaat tgttacgggt gcctcatcag gaataggtga agcaaccgca cgcgctttcg
    58741 gactcgaagg tgcaaaagtc gtactggctg cccgtcgcgt tgaaaaactg caagccatcg
    58801 ctcaggaaat taatgccttg aatacgggtg cagagacttt ggttgtgcca accgacctct
    58861 ccaaactggc cgacattcaa aacctgatcg ctcaaaccct tgcacgtttt gggcgcattg
    58921 atgtgctggt caataatgct ggcattgggc gagtggactg gcttgaaaac cttgaccctg
    58981 tttgcgatat tcaaactcaa tttgatgtga atgtcatggg tatgatccag gcaacacggc
    59041 tggccttgcc aaccatgatc caacaacgtt caggcaatat catcaacatg tgttccattg
    59101 ccgggttggt ggctacaccg acatattcca tttatgcggc gtctaaacat gctgtgcagg
    59161 gattttcaga ggctctgcgg cgcgaggtca agccgtgggg aatccacgtc tcacctattt
    59221 atcctggtgg agttgccaca gaatttgccc aacatgctgg catcaagcgc aagaccaatg
    59281 ccaaaacacc ggctttcatg ctgctgaccg cccaacaggt tggtgatgcc attgtggatc
    59341 tcgttcgtcg cccccgccgt atgcgcatca ttccctggtt gtgggctgtg acggtcttcc
    59401 tgaataaatt tctgccaggc attgtagatc acaccaccat taatcgcttc accatccccg
    59461 agcgaaaaga cgaattggca actggatgac atgataacaa ttttgtggaa taatataaca
    59521 aatttgtgac acgtcgtctg gaggtagcat gggtttctta tccaatttgt tcggaccatc
    59581 ggtgccaaaa cttggcgcaa tggaagtaag tgaaaaaatg aaatttggaa aacatccgat
    59641 tttggtggat gttcgccaac ctgaagaatt tcgtcagggg catattgcag gggcgaaatt
    59701 aattccgctc aatgaattac acaagcggat caaggaattg ccgcaaggcc gtgaaattat
    59761 ttgcgtctgc gcttctggaa gccgcagcag ttccgcagca aaaatgctgt ccagggaggg
    59821 gtacaccgta tttgatttgc aaggcggcat gcttgcctgg cggcacgcaa aattgcccat
    59881 tcaaaaaggc tgataactta aataaaaact gtcgggatac tcccgacagt ttttatttaa
    59941 tcccttgctc ccgttgcttt gaggtctttc aaattcaatt gatatccgat gcggccatta
    60001 tattcgttca cttcatagtt gaacaaaata tccacccgtg gcggcatttt cttttgccag
    60061 tcaccgagct tgaacccgat tgcatcgtgg gtgtatcctt tggcatcttc aaggatcaat
    60121 ttcaagtgtt tcgcatctga ccccactgtg cgcgcgctct ttaccttgat gttacgcgcc
    60181 acaaatgatg cgtttggatt tccatatcct gtgggctcaa gatatcgcaa tgcattttca
    60241 aataactcag ggcggatatc gacgagggaa acttccgcgt ctgccgtaac tgtgggcctg
    60301 agatcttcgt tcgagagttt gtcagcggcg atggttttta atcgcgatac caactcgggc
    60361 aggttttcat tttttaccgt gaaccctgcc gctgctgcat gaccaccgtg ccgcacaagc
    60421 aggtctgcac actgatcgag tgcttcggtg atatggaact cgggaatgga tcggcacgac
    60481 ccgcgtgttt cttccgcgcc tttgtaggca acaatggatg gccgatagta ggtttcagtc
    60541 aaacgcgagg cagccaggcc aaccacacct gagttgaaat cttcgtgtgc ggcaaaaagc
    60601 agataggcat ttgggtcgtc attcattgcc atgtcttcag cgcggctttg aatttcacgc
    60661 gtaatacgtt gacgttcgcg gttttgcaag tctaaagttt gggcaagctc ccctgcgcgg
    60721 aaaacatcct ttgtggtcag caattcaaag gcggccagcg cttcttttaa tcttcctgct
    60781 gcgttcaagc gcgggccaag cgaaaaacca atgtgtcctg cattgacctt ttccaacttt
    60841 acggctgaaa cggcggcgag agaggataat ccctgtctca aggttcgttt catttgtctg
    60901 agccctctgc ggaccaacat gcgattttca ccgacgagcg gggccaggtc tgccacggtt
    60961 ccaagcgcaa ccaggtccaa cagagagtcg aggtgggggg ccagcggttt gccttgaatg
    61021 ctgaaaagag cctctgcgat tttgtaggca atgcccacac ccgccagttc tttatctgga
    61081 tagatatcgc cgtgttgttt ggggttgatg acagccagtg caggaggcag ttcgccttct
    61141 gcggggtggt ggtggtcgct gataatgaga tccaggccga gggtccgcgc atgaagcgct
    61201 tcatttggag agcgaatgcc gcaatcaacg gtgatgacca gtttgacgtt atccgcttga
    61261 agtttgtcca tcgcttcgtt gttgaggcca tacccctctt cgaaacgatt tggaatgtat
    61321 ccgcgcacat cggcattcag gtattcgagc gcctgcacca ataaagcggt ggcggtgacg
    61381 ccatccacgt cataatcacc gtaaatggcg atgggttcgt ggtggtcaat ggcaaattga
    61441 atgcgttcga tcgcttccct cattcccgtc attagaaacg ggtcgttgtt ggaatcgggt
    61501 ttggcattca ggtaggcccg cgcttctgca tcggtggcat acccacggtt gaaaaggatt
    61561 tgcctcaaaa tggacggaaa tgcttgcagc gcagaatcag cttcgggtgt gatcgaggtc
    61621 gggatgaccc agcgtttggt gttcgatgtg tcaggtgtca tattaaagtt gtgatgcctg
    61681 gagtataaac gttcaaccag cggcatggaa gggtgctgta taatttgggc ttattcaaag
    61741 gagatgataa tgaaggcact tgcgtatgat gaaaagacag cgatggtgat ggtttatacc
    61801 gattccatgc ttgtgcgtgg ggaagtggtt gcaaaggaaa atgtgagggt aagtatctgg
    61861 ctgcgtacgc agggtgtgcc caattacatc caccttcatg gggcgcaggt catctctttc
    61921 agtggcggca gcgcaaaaaa ttatacacag gctgagatct ttattcccac agtgcaggtt
    61981 tgtgcatttc atctggcgcc gcctgctcat gacccgctag attatgatgc ttccgagatc
    62041 aaccgtgtca tgcagcctgt gaatattatg gtcggctctt ttatgctgcg tggaaaaatg
    62101 cgcatctctt cacaaactga atttgggacc agtctcgatg tgatgcgtgc ttcgtgggcc
    62161 tccgtgtatg aagtagaggt gagcaatccg ttcctgccgc agttcaacat gcaggtgcct
    62221 atgcttctgg ttaacccaag ccatgtaact tttgggcttg cataaatgac cttctaaatt
    62281 ggaacttggc aaaacaagcc acaatggtat aattcgcagg ctttcggggc gtggctcagc
    62341 ccggtagagt gcacggttcg ggtccgtgag gtcgagagtt caaatctctc cgccccgaca
    62401 agtagtaagg agactcccaa cccgagggag tcttttttgt cgctgttggt ttttctggta
    62461 aaatgtgccc ctgtgttttc agctcgcaag cgcactctgc ctgtctgaaa cacaatgtac
    62521 cctgatgggt agaaaacatt ctaaaggagt aagtaaaaat ggttcgtctt cgtttacgtc
    62581 gtattggtct caagggccaa cccacgtacc gcatcgtcgc tgctgataag gaagccccac
    62641 gcgatggtcg ctttttggaa attttgggcg tgtataaccc acgcaccaac cctgccacca
    62701 tcaacctcaa ggaagatcgc atcttccact ggatgaagaa tggcgccctg cccaccgaat
    62761 cggttgccca ggttttcaag gcctccggta cccaggcacg ctttgaacgt ttgaagaagg
    62821 gtgagtcgct tgaggcgctc gttgccgaag ctgctgaggc ccaggccaag cgcgcggctc
    62881 ccgtcaagac ccgcaaagat taatcgcttt ccaaaagagc attaaccaca gggcgtatgg
    62941 atgccacaga gaagatgttg tacaattctt ttgtggtctt tgcgctctgt ggttattttt
    63001 ttatcaaggc aggtagaaaa tgaaggaact gatcgaatat attgccaaat cccttgttga
    63061 ccatcccgag gatgtgcagg tacgtgaaag cggcggtggt tcgcgtgtgc gtatcgaact
    63121 cagcgtttca aaggacgata tgggacgcgt catcggcaag agcggcaagg tggcaaactc
    63181 catccgcact ctgttgaagg ttgcagctga gcgttcgggc aagcaggcga cattggatgt
    63241 gatggaaccc caatgacggc gaagcagaaa tctacaaagc agacagaatc aggctcgccc
    63301 gcgggcgagc ctgtctattt ggtgatcggc tatttgcgcc gctcccatgg tatccatggc
    63361 gagatcatta tggatctgca caccgatttt ccagagcgca tcaaggcggg acgcaagatt
    63421 tttattggtg aaaatcacca gccgcacact ttgggcggtg tccgtgaaca tggcaatggg
    63481 gtcctggtca aattacgcgg tattgaaacc cccgaagagg tgggcagatt ccgcaatcag
    63541 tgggtgtatg taaaagctgc agatgttcca cctttgcctg aagggcagta ttacaaatat
    63601 gaattgatcg ggttggatgt ggtggatgat caaggcaacg cgctcggaaa acttaatgag
    63661 atcctggaaa cgggtgcgaa cgacgtgtac gtggtcaaaa atgaatctgg caaggaaatc
    63721 cttctgcctg ccattccccc ggtcattctg gatgtggata tgggtcagcg ggttatgaag
    63781 gttcatctgc tcgacggttt ggtggacggt taaatacgac gaagcaaaag tgggtttatg
    63841 gacgatagaa aatattgggt cggcttcaat ttgatcaaag gcattggcgc agtgcggatg
    63901 cagggattgg ttgcgtattt tggtgacctt gcttctgcct ggggcgctga ccctgccagt
    63961 ttggctgaag caggcctggg ggcgaaagtg atcgagcggg ttcttgctgc acgtcaaaaa
    64021 gtggaccttg atcaggtctg gaacaagatt caattacagg gcatcaaaat cctcacctgg
    64081 caggatgaac aatacccatc tcgtttgaag gaaattgacc agccgccgcc cgtgttatac
    64141 attcgcggtg aatatctgct ggatgattta ttcgctgtgg cgatcgtagg cacacggcgt
    64201 gtgaccccgt atggtcggca ggtgaccgag gaaatttcct cttttcttgc ggccaacggc
    64261 atcaccgtgg tgagcggatt ggcgcggggt gtggatgcag tggcgcatca aactgcgttg
    64321 aaggcggggg gtagaaccat cggcgtttta ggcagtggtg tggataaaat ttatccgcct
    64381 gagcatcaaa aactggcgga gcaaatgatg gagcgtggtg cggtcatcag tgattacgcg
    64441 cccggtacac cgcctgatgc atccaatttt cccccgcgaa atcgaatcat ttctggattg
    64501 tcattggctg tggtggttat tgaagcaggg gagaccagcg gtgctttgat cactgcggaa
    64561 ttcgctgccg agcagggacg tgagatcttt gctgtgccgg gcagtattct tgccccacaa
    64621 agcaaaggta cgaacaagtt gatccagaat ggggctttgc ctttgttgag tgccaatgac
    64681 ctgatgcagg ctttggatat cacccgtatc ggtgaacata aaaccgcccg aaagatactg
    64741 cccgccgatg aaaccgaggc gcgccttttg ggggtccttg gtcatgagcc cttgcatgtg
    64801 gatgagattc gcatgcaggc tgatttgccc attgaaaaag tatctgctac actggctctc
    64861 atggaattga aaggtatggt tcggcaggtt ggtggtatga attacgtatc ggtgcgtgaa
    64921 gcacagtcgg agtacaaaac ttaaggctcg gtgttgcgaa ttgctgttcc agagtgattc
    64981 gcaatgcgca aaggaatcta ctatggaaaa tacctatgca gtgatcatgg ctggtggtgg
    65041 tggtacacgt ctttggcctg tttcgagaaa agaacgcccg aaacagttgc tgcctttgct
    65101 tggtcaagat accttgtttc aaagtacggt ggcacgtttg aaaaacatgt tcccgtatga
    65161 gcgcattctg gtggtgacag tggaggagca ggcccgcgaa atgcgcttgc aggctcctga
    65221 aatccccgag gaaaattaca tcattgagcc cgcgccgcgt ggaacggctt cagtggtggg
    65281 gttggccgcg gcggttttac acaagcgtga ccccaaggcc aaaatggcga tcctgccgtc
    65341 cgatcatttc atccgcaatc gcgatctctt ccattatttg ctcaatgcag catttgatgt
    65401 ggcggaaaat ggatatttgg ttacgcttgg cattacacca acccagcctt ccacggccta
    65461 tggctatatc cagcagggcg aatcgttgga tggcgaatat aaataccctg cctaccgtgt
    65521 aaaacgtttt gtggaaaaac ctgatgaact tactgcgcag aaactgctcc ataccggcga
    65581 tcattcctgg aacagcggca tgttcatctg gcgggcggat gtgatccttg atgagatcga
    65641 taagcaaatg ccagagttgg gcggggcatt gaagaagatc gcggctgcat ggggagccga
    65701 tcagcaaggc gctgtcttgg atgagaattg gcagacgctc aaaactgaaa ccgtggacta
    65761 cggcatcatg gaacatgcgg aacgcgtagc ggtccttcct gcgggtgggc tgggatggag
    65821 tgatgtgggg atgtggtctt cgctgtttga ggtgcttctt cctgatatga atggcaatat
    65881 ctccactaat agcagcctgc atttggcaca tgaaacgcac aacactcttg tttatggcgg
    65941 gaacagcgat cgcctgatcg taaccatcgg cgtggagaac atggtcattg tggatggcgg
    66001 ggatgtggtg atgatctgca agaccgatca tgcgcaaaaa gtaaaagacg tggttgggca
    66061 tttgaagaaa cataatcagg aaaaatactt gtagccaata actgcaggag atatctcctg
    66121 cagttcgatt tttaagggga ttttcgatgt ttaaggcgca ggcaaatcgc gccttgaaaa
    66181 attgcatttg atatatttcg gaaaattgcg tagaatgcgt gtccagtatg gatggaaatc
    66241 aggttttgat atacaaacgc attttttgcg tggaggatag atggaagctt attgtatgaa
    66301 atgcaagaca aaacgggaga tgaaggaccc agttgcagga ttcaacgcaa agggttcgcc
    66361 cgttacaaca gggacgtgcc ctgaatgcgg cacaaaacta tatcgtatgg gtaaaactga
    66421 tgcccatgcg gatatggttg cacctccgcg ccctgagaag gtggttgtgc gtgatggcaa
    66481 gctggtgatc gttgaatcgc ccgccaaggc aaagactgtc gggcgttttc ttggcaaggg
    66541 atataccgtg cgtgcatcgg tggggcatgt gcgtgacctg ctcaaatcgc agttgtctgt
    66601 ggatgtggaa aataatttcg agccgaaata ccgtgtgccc aatgaaaaga aggatgtggt
    66661 caaggagatc aagaagctcg cctcgaccgc tgaagaaatc tatctggcga ccgaccctga
    66721 tcgcgaaggc gaatccattt catggcattt gatggaggcc gcccaaatcg aacccgaacg
    66781 taccaaacgc gtggtctttc atgaaattac cgcccctgca gtggcagagg ctttttcaca
    66841 cccgcgtgag atcaatatgg atctggtcaa cgcccagcag gctcgccgtg tgctggaccg
    66901 tcttgtcgga tacagcatca gcccgatact ttgggaaaaa gtacgtggac gtttgtcggc
    66961 ggggcgtgtg cagtcggtgg cactcaggct tatcgtggac cgtgaacgtg agatcgacga
    67021 attcaagcct gttgagtatt ggtccattca tgccgaatta aagcatggca gtgcgaagac
    67081 ttcgttcctt tcgaaactgg ttcgtattga cgataaggaa cctgaactac caaacgaaga
    67141 gaccgtcaga ccgatcctgg tggatatgga aaccgccgcc tatgccgtga caaaggtcaa
    67201 acgcggcgaa cgcaggcgca aacctgcagg gccatttacc acatccacgc tgcaacagga
    67261 ggcttcgcgc aaattgggct ttacggccaa acgcacgatg ggtctggcgc aaggcctgta
    67321 cgaaggtcag gatgtgggtg agggcggcac gaccggtctc atcacctaca tgcgtaccga
    67381 ctcgatgaat gtctcggcgc tggcgcaaaa tgaggcgcgt gaatatgtgt cgggtaaata
    67441 tggaagcgat tatcttcccg ctgaagcgcc gatgtacaag acaaaatctg caggggcgca
    67501 ggaggcgcat gaagccatcc gtcccacctc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    67561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    67621 nnnnnnnnnn gaagagaatg taaaaattcc cgctggaatt gccgaagggc aggagcagga
    67681 attggtgcgg ctcatccctg agcagcactt tacccagcct ccgccgcgtt tttctgaagc
    67741 ctcgctggta cagacccttg aagagaatgg cattggccgc ccttccacct acgctccgac
    67801 aatttcgacc attcaacagc gtggttatgt ggagcgggtg gataagcgcc tgatccccac
    67861 cgatacaggt ctacaggtaa atgacctgat ggtgcaatac ttccctgaag tggtggattt
    67921 taatttcacc gctcacatgg aagaagacct cgataagatc gcagacggcg agatgatctg
    67981 gaccgatgcg atccgtgagt tttacaaccc gtttgctgaa gatgtgaaaa aagcgcaggc
    68041 cgaaatgcct gtcaccaaat ctggccctga accgatcggt cgtgcctgcc ccacttgcgg
    68101 caaggaattg gtcatccgct atgggcgttt cggtaaattc atttcctgca gcggctttcc
    68161 agaatgccgc tacaccgaac cgtggctgga gaagatcggt gtctcatgcc cgaaagacca
    68221 cggcgatctg gtggagagga agacgcgcaa aggccgcacc ttctttgggt gtatgaacta
    68281 cccgaactgc gatttcactt catggaagag gcctattgcc gttccatgcc ccaagtgcag
    68341 cggattactg gtgatcgcga acaagcgcga agcacaatgt accaattgtt ccgagtcctt
    68401 cctgctggaa gagatcatgc ctgagaccgt ggaataaacc atgacaaccg ctctgtaaaa
    68461 tgagcggtcc caacttgaag gagaagaaaa aatgcacttt acaccagttc catacctcga
    68521 ccccggctcc gggagtttca ttattcaaat tttgatcgcg gccttgttag gtctgggagt
    68581 tgccttgcgt gcttcatggg gcaagatcaa gggatggttt ggaatcaagc cgaaagcgga
    68641 agatgaggat ggcgaatctg ccgatgccag caactgaaca gctttccgct tcttttcgtg
    68701 acccaagcgg gtttctgttt tctcgtaacg gggtgcttta ccgacaaatc aaccgcgcct
    68761 atgaaaagga ttatgcgcgg ttaatggatt caggtctgta tgagaagctg gtcaaggctg
    68821 ggctgctgat tccgcatgtg gagtcggatc aggctccagc agagtcggct tcggttttta
    68881 aagtcatcca gcctgaacgg gttccgttca tttcctaccc gtatgaatgg tcattcagcc
    68941 aattaaaaga tgccgccctg acgactctgg cgatccacaa acgtgcaatc aaggcgggca
    69001 tggccttgaa ggatgcaagc gcatacaaca ttcagtttgt gcgcggaaaa gcaactttga
    69061 tcgatactct ctcttttgag atttataaag agggtgagcc gtgggtggcg tatcgtcaat
    69121 tctgtcagca ttttcttgcg ccgctggctt tgatggctta ttgcgatgtg cgtttgagtc
    69181 agttgttgcg ggtgtatatt gacggtgtgc cgcttgatct tgccagcaag ctgctgcctg
    69241 taaaaacgaa gttcaacttc ggcctgctta cgcatatcca tgtccatgca ggtgcgcaga
    69301 agcgatattc tgacaaggcc gttgagactc gcaagggaac cacaggagtg agtcagcagg
    69361 caatgatcgg tttgatcgaa agcctcgaag ccgccgtcaa aaaattgacc tggaaacccg
    69421 ctggcacgga atggggcgat tattacgaaa atacaaatta tacagatggc gcgttcgagc
    69481 ataagcggca gcttgtgcgt gaatggacgc ttgagaaaaa gcccacactg gtttgggatc
    69541 tgggcggcaa tacgggtgtc tttagtcgtg aggcggtttc ttccggtgca ttcgcggttt
    69601 cttttgatat cgaccctgcc gcagtggaac aaaactatcg aaccgtaaga tcaaaaaagg
    69661 aacagaacat cctgccgctg gttttggact tgaccaaccc cagtccgtct ttgggctggg
    69721 atcacatgga acgcgactcg ttcggtgcgc gcggccctgt ggacatggcc ttggcgctgg
    69781 cggtcatcca tcacctggcg atttcaaata atgtaccgtt gccgcagttg gcatcgtttt
    69841 ttgcggcgcg ttcgaaatgg ctggtcatag aatttgtccc taaatctgat tcgcaggttc
    69901 gcaggctgct gacttcgcgt gaagatattt ttcccaatta cacccgtgaa gggtttgaaa
    69961 gcgctttttc aaatcgcttc accattcata aagcggaagc ggtgcgagat tcagagcgta
    70021 ttctttactt aatggaagcg cggtaaccat tctgatatgt gggtggtaaa aaattggatg
    70081 tataattgcc caaataacat tatacaaagg agcctcacat gcagtttgtt gtagcattct
    70141 tgaaaaaact gccggccgtc ccaattgcgc tgttcgttgg cgggttggtt ttagggctgg
    70201 tgtttgggtg ggtgatctgg cccacggagt acacaaatac aaccccagat tccctgcgcg
    70261 ccgacttgca ggaagactat ctgcgcatgg cgatcgactc ctaccgtgta aatcttgatc
    70321 ctgaccttgc cattcaacgt tggttcaacc tggggatagg tgctgatcag gcatttaaaa
    70381 atattcaggc caacccgggc aagctggatc ccgcagtaat tcaagcatat ggcgaaacag
    70441 ttacgaagat ccttgctaca ggtgtgcagc caccggtaaa tacaggcggc agcgctacac
    70501 tgcttaggag catcttcctg tggggtggtc tgctcgttct gttagggatt ggcggttatg
    70561 gcggctggta tatgtatcgt ttgcttggca agcgtggcag cggcgaagta acacccacca
    70621 tgcaggccgc ggaactcaac cgcagtgcag agaagaccaa cttcgaacag cttgggctgg
    70681 ctccgcccat tacacagacc atgaccacat acgtgctcgg tgatgatctt tatgatgaat
    70741 ccttcagcat tgatacacag gctggcgaat tcatgggcga atatggtgtg ggtgtttcag
    70801 aggccattgg tgtaggtgag ccgaagaagg tcactgcact ggagatttgg cttttcgaca
    70861 agaacgatat caagactgcc accaaggttt tgatgtcaca acatgctttc aacgacccaa
    70921 cgatccgcgc ccgcctcgaa cccaagggtg agttgatcct ggttgagccg caagctcagg
    70981 tgctgttgga gaccgccacc ctgcagttgc tcgcaaccgt tgtggacctg gagtatggtc
    71041 acggccccca accagctgac agttatttcg aacgcattac cctcgaattg gccatctggc
    71101 ctcgacaggg ataatcaaaa cataaaaaat ccgcctccat tttcggaggc ggattttttg
    71161 ttattcaatg ctgataatct tgaatttgat cttcccaccg ggcgtttccg cttcgacaac
    71221 ctgaccgacc tttttttcca tcagtgcttt gccgatgggg gactcgatcg aaatctttcc
    71281 cttgcgcgga tctgcttctg tagcgccgac cagatgatag gtctcagggt cataatcggc
    71341 ttcctgaatg gtcacgcgtg agccgaccga aaccacatcc ttggtttgag aattgtcaat
    71401 aatgacggcg ctcctcaaaa tggcttcaag ctcctgaatt cggccttcga gaaaaccctg
    71461 atcttccttg gctttgtggt aatcagcgtt ctctgaaagg tctcccattt gaatggcaga
    71521 acgtaatctt tgcgaaagtt cctcgcgccg ggggcctttg agttcctgca attcagcggt
    71581 gagttttgct tcgccttctg gagtaaggta attaatggat gacatggtaa tcgctccttt
    71641 ctgaaattgt tataagggtg gtttgacggg gacgaaaata actttgttcc cccttgattg
    71701 aaaagattaa ggcttttcag aggggttgaa cagcttttga tgttcttcaa cggcatagcg
    71761 gtcggtcatg cctgcaatgt aatcgcagat ggtgcgttcg agtccgcgct tttcgatgaa
    71821 gaattggaca tggtcaggca gcatggacgg ttccgcctga taggcatgga acaactcaga
    71881 gatgacccgc tccgctttga tctgcatccg caccacgcgg taatggcgat agagcttttt
    71941 ataaagcagg tctttcaatt cgcggttacg gcgctgcatc tcttcgctat aaccgatgat
    72001 gttgtacttg agcttttgaa tatccaatgc ggtctttgcc ttgctgtctt tcaggcggat
    72061 atctgtcgct tgcagcatat cggtgaccat cagcccaacc agttgacgga tcatgcgatg
    72121 gcgttccatt tcgctcaacg tatgtccctg ccatttgtag gtttcgcgca ggatttccca
    72181 cagcgagatg ccttcaagga tgtgtggggt gatcattccc gagcgcaggc cgtcgtcaag
    72241 atcatgtgtc gtgtaggcaa gctcgtcggc gacatttgca atttgagttt cgagatttcc
    72301 gcgcagatcg gggttgaaat cccgtgcgtc agaaatatcg tattccgatt cgtgcttgac
    72361 cattccctcg cgcacttccc aggtaaggtt taagccaggg aattcgggat accgctgttc
    72421 cagttcggtc acgatccgca aagactgttt gttgtggtcg aagccgccaa attccttcat
    72481 cagccgtgcc agtgcaactt cacctgaatg cccgaatggc gaatgaccaa gatcgtgcgc
    72541 caggcagatc gcctctacaa gatcctcgtt ggcgcccaag gcgcgggcca atgtccgccc
    72601 gatttgagca acttccagtg tatgcgtgag acgggtacgg aaatagtcgc cttcgaaatt
    72661 gataaaaacc tgcgtcttgt attccagcct gcgaaaggca gtggtatgca gaatacggtc
    72721 acggtcacgc tgaaaggaag ttcgatagtc gggttcgcta tccagatagg cgcgtccctt
    72781 cgaatccttg ctgcgcattc cataggaagc aaggctttta tcttcgatct cttccaattg
    72841 ctgacgggtg aacatgattt ccttttccga ttctattgcg tgccatggcc ttagacaaaa
    72901 ctgtcattgc gtgctttgag ttttctcaag ttctacgcaa tgacattgaa tttgtggggc
    72961 gagagggggt cgaaccctca cgttgttacc aacgacagat tttaagtctg ttgcgtctgc
    73021 cgattccgcc atcgccccgg cgattcatat tttaccttaa tttcacgctc atgcattcaa
    73081 tggctctcat gacaagtgaa taaagcacat taattatcta catggattgt ctccattctt
    73141 tgccagctcc gttttttaag ttgcactttg gtctaaaatc gagtaaattt acctgatagc
    73201 acgtacgttc tacacaggag aagattcatg gcagactcaa atttttacct tggccgattg
    73261 tttgatacaa aaacagcgaa ggtcaccact caacccgttg aatatgaccc cgcagatctg
    73321 accacccacg ctgttgtgac aggcatgacg ggctctggta aaacaggctt gtgtgttgct
    73381 ttgctcgaag aagctgcttt gcaaaatgtc cctgccatca tcattgaccc taaaggcgat
    73441 ctaaccaacc tgcttttgca tttccccgac ctgctcccgc aggattttca accgtggatt
    73501 gaccccgaaa tcgtccgccg cgcaggcacc acccctgaaa gagctgcaca ggaagccgcc
    73561 gaatcctggc gtgatggact gaccgagtgg ggcattggtc aagaccgttt gctggctctg
    73621 aaaaatgccg cacagttcgc catttacacc cctggttccg attcaggcat ccctgtcagc
    73681 gtactttctt cgctcgctgc acccgatctc gattgggaga ccaaccgtga ggtcctgcgc
    73741 gaaaagattt ccagcaccgt caccgccttg ttgggccttg tcggtatgac cgatcttgac
    73801 ccgctccgct cgcgcgaaca catcctgctc tcgaacatct tcgagacgca ctggatcaac
    73861 ggcaaagacc tcgacctcac cgaattaatt ctacaaacac agaccccgcc attccaaaac
    73921 ctcggtgcct tccctgtgga tacattcttc ccgcccaaag accgcatgga actggcaatg
    73981 gttttgaaca acattctggc tgcacctgcc ttcgaaacct ggcgtgaagg tgaagagctt
    74041 gatgttcaaa aactgctatt catgcccgat ggcaaaccgc gccacagcat cttctacctt
    74101 gcccacctct ctgatggcga gcgcatgttc tttgtcacac tcctgctttc cgctgtggaa
    74161 acatggatgc gtacccaaaa aggctccacc gctctgcgtg ccttgcttta catggatgaa
    74221 atctacggct atcttccgcc tactgctgtg ccaccctcaa agggtccatt gttgcgaatg
    74281 ttgaagcagg ctcgtgcttt tggcctcggc ctcttgctcg ccacccaaaa ccctgtagat
    74341 atggattaca aagccctctc gaacacgggc acatggttca ttggcaaact gcaaaccgaa
    74401 cgtgataaga accgcttgct cgacggcctc gaaagcgccg ctggcggcat cccgcgcgct
    74461 g
//
LOCUS       DAOQJC010000004        61880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_177_length_61880_cov_8.155487, whole genome shotgun sequence.
ACCESSION   DAOQJC010000004 DAOQJC010000000
VERSION     DAOQJC010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 61880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 61880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61880
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177_length_61880_cov_8.155487"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..778)
                     /locus_tag="PLE39_01150"
     CDS             complement(104..778)
                     /locus_tag="PLE39_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE66956.1"
                     /translation="MTRILVVDDERPIRRFLKASLGNEYEVVEAVDGEDAIRVTATEK
                     PDLVILDLGLPDMDGVEVTRRLREWTQIPIIIVSVREQETDKISALDAGADDYLTKPF
                     GAGELMARIRVAMRHASSPETQAVFESGDLLVNLSDRIVKVAGEEISLTPNEYDILRA
                     LVQHAGRVMTHHQLLRTVWGNAYENEAHLLRVNMSNLRRKIEADPARPRHIITEPGVG
                     YRLKIA"
     gene            complement(775..3441)
                     /locus_tag="PLE39_01155"
     CDS             complement(775..3441)
                     /locus_tag="PLE39_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HNE66957.1"
                     /translation="MQQRPDPDELLKHIQPEEHGRGKLKMFLGYAAGVGKTYAMLEAA
                     HQRKAQGVDVVIGCVETHKRADTDELVEGLEVLPRKQVNYHNVTLPEMDVDRILARKP
                     ALVLVDEFAHTNAPGSRHPKRFQDVEEILNAGIDVYTTLNIQHLESLNDIVAQVTGVI
                     VRETVPDRVIDEASEIEVIDLPPDELLTRLKEGKVYIPEQASRAIDKFFRKGNLTALR
                     EMSLRRAAERVDDQMRSYMQTRAISGPWAAGERLLVCVSPSPLSERLIRSARRLADEL
                     NAEWITVYVETPQFAAMSQEKRDRVAKILQLAEDLGARSYTLSSNGSIESTAQAVIDF
                     AHKNNITKIIAGKPLRPRWQEFLRGSLVDKLIHKSGDIDIYVVTSMDVPAFPADENPL
                     KLHSPLYKYFLSMLLVGFATIIGFVIGGRIEPTNLVMVYLLAVVVASVYLGRGPAVLA
                     SLLGVLAFDFLFVTPYFTFAVSDTQYIITFIALFLVGMVISQLAARASEQAEAARQRE
                     AETAELYDLSRDLAVSAELESILAVLQKHVEKIFSRTVAVLLPTENRLTSYILSTGLE
                     LSDEEAAVADWVYRHGEAAGRNTNTLPAAQLRYLPLKTARGVVGVLGVGKPNTIERDL
                     TPAQRRLMEAFANQCAQAIERVRLAEEARQIKLLQSAEKLQNALLNSISHDLRTPLVS
                     ITGALTTLEEQASELESPAHRSLVETAREEADRLNRLVGNLLDMTRLEAGALKVKCEP
                     SDIQELIGSAISQMESRLDRRRIEVQISDALPLVPLDFVLIVHVLNNLIDNALKYSPE
                     GSPLEINADLVKDEIQISVTDNGIGIPHEDLERVFDKFYRVQRPENVTGTGLGLAICK
                     GIIEAHGGRIWAKNNAVGGSVFTIALPMEEAK"
     gene            complement(3447..4010)
                     /gene="kdpC"
                     /locus_tag="PLE39_01160"
     CDS             complement(3447..4010)
                     /gene="kdpC"
                     /locus_tag="PLE39_01160"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HNE66958.1"
                     /translation="MKTHLRPAFTILTLLTVLTGLVYPFLVTGLAQVFFPQQANGSLI
                     SVNGTVYGSELIGQSFDDPKYFWARPSAAGYNAAASSGSNYGPTNPALMDAIQARIAA
                     LQTADPENTLPIPVDLVTASGSGLDPHISVAAALYQVHRVAAARGLSEAEVRSLVEQN
                     TEGRQFGFLGEPRVNVLMLNLALDGIQ"
     gene            complement(4007..4180)
                     /locus_tag="PLE39_01165"
     CDS             complement(4007..4180)
                     /locus_tag="PLE39_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66959.1"
                     /translation="MKRNHHLLPYFIYTSAILGFTAAIAPDKLNIPTNFRPWVFLASI
                     AWAVAVLSGVFTA"
     gene            complement(4188..6272)
                     /gene="kdpB"
                     /locus_tag="PLE39_01170"
     CDS             complement(4188..6272)
                     /gene="kdpB"
                     /locus_tag="PLE39_01170"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HNE66960.1"
                     /translation="MSIQTRKHNSFRREIYQRAVTEAFIKLNPRLMIRNPVMFVVEVG
                     SALTTVLWVQALLGKGEASSGFIGAIAIWLWFTVLFANFSEAVAEGRGKAQAESLRKA
                     KQDTPAKKLKKTQLDGATSEHEVISSTLLRKNDLYLVVTGDILPADGEIEAGIASVDE
                     SAVTGESAPVVREAGGDRSAVTGGTRLLSDWLIVRVSADPGQGFLDRMINMVEGAKRQ
                     KTPNEIALNILLAGFTLIFLVVCATLLPYSIYSVEAAGKGTAITITVLIALLVCLIPT
                     TIGGLLSAIGIAGMDRMIQRNVIAMSGRAVEAAGDVDVLLLDKTGTITLGNRQAVEFI
                     PVKGVMANELAEAAQLASLADETPEGRSIVVLAKEKFGLRGRTMNAPAGMHFVPFTAQ
                     TRMSGVDFDGISIRKGAPDTMLSMADFNGHPFPADLRSSVDSIARMGGTPLVVTRNTK
                     ALGVIYLKDIVKGGIKERFAQLRKMGIKTVMITGDNPLTAAAIAAEAGVDDFLAQATP
                     EAKLKLIREYQSGGRLVAMTGDGTNDAPALAQADVAVAMNTGTQPAREAANMVDLDSN
                     PTKLIEIVEIGKQLLMTRGALTTFSIANDVSKYFAIIPAAFASTYPVLGALNFMKLAT
                     PQSAILSAVIFNALIIIALIPIALRGVPYRAKPADQLLRDNLLVYGLGGLIAPFIFIK
                     LIDLILVWIGVA"
     gene            complement(6296..8014)
                     /gene="kdpA"
                     /locus_tag="PLE39_01175"
     CDS             complement(6296..8014)
                     /gene="kdpA"
                     /locus_tag="PLE39_01175"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HNE66961.1"
                     /translation="MNTNNLLQLMFFMGTLLLLVKPLGSYMARVYQNERIFLDRVLGP
                     VERLIYRASGIRPTEDMDWKSYAAALLVFNMLGLLVVYAQQRLQLALPFNPQGFSAVT
                     PDSAWNTAVSFASNTNWQSYGGETTMSHFTQMLSLTVQNFVSAATGMAVLVALIRGIA
                     RHSGKGIGNFWVDLTRSTLYILLPLSFILAMLLVSQGVVQTFNGYRTVSPLQSAEQVI
                     AVGPAASQIAIKQLGTNGGGFFNANSAHPFENPTPFSNFLEMLSILLIPAALCYTFGQ
                     MVGDLRQGWALLTVMTIIFLVLVGITIWSELGGNPALPSLGIDQIQSASNPGGNMEGK
                     EVRFGIVNSALWATATTAASNGSVNAMHDSFIPLGGLVPMWLMQLGEIIYGGVGSGLY
                     GMLAFVIVAVFISGLMVGRTPEYLGKKIEAYEMKMASLIILIPVTVVLTGTALAVSIP
                     DGRAAIYNPTAHGFSEVLYAFSSAGNNNGSAFAGLSANSPFYNLSLGIAMLISRYWLA
                     VPVLAIAGSLVRKKKIPASAGTLPTHTPLFMAWLIGVITIVGALSFIPALALGPIVEY
                     LTIIGG"
     gene            complement(8011..8103)
                     /gene="kdpF"
                     /locus_tag="PLE39_01180"
     CDS             complement(8011..8103)
                     /gene="kdpF"
                     /locus_tag="PLE39_01180"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HNE66962.1"
                     /translation="MNTLYAIVGIITLLLFGYLVLALIKPEWFG"
     gene            8356..9288
                     /gene="ricT"
                     /locus_tag="PLE39_01185"
     CDS             8356..9288
                     /gene="ricT"
                     /locus_tag="PLE39_01185"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNE66963.1"
                     /translation="MQTNIIGVRFTKIGKIYHFDSSTLPDVKQGEHVIVDTTRGKHLG
                     EVVQVLTETPPQPEGGWKAVERRATPRDLLLQQSWQSRQTEAMINCRARASELHLRDV
                     KIVTAEYNYDGSRLTFLFSTESEDKVDLKSLKKDMQQIYPNAQIEMRQIGPRDVAKFL
                     GGMGACGIETRCCSKFLTDFSPISIKMAKEQGISLTPNEITGMCGRLRCCLIYEYEQY
                     VEARKTLPKRNKRVVTPKGEGKVIDVVPMSDKVVVLIESGVDRPQQMTFAREEIEPWD
                     ELEALRRKSQAPCDRHEGGGCTCGKAPRNNNNKN"
     gene            9343..10071
                     /locus_tag="PLE39_01190"
     CDS             9343..10071
                     /locus_tag="PLE39_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNE66964.1"
                     /translation="MTLQPENWTSPQNILVILAHPDDPEFFCGATLARWARDGHKITY
                     LLLTCGDKGFNPTTNPEMTPEKLCGLRHGEQNNAAKIIGANSVHFMDLPDGYLIPDLN
                     LRRDIVREIRRHKPDILVTCDPQTLFATYGINHPDHRACGQVVLDAVFPAAGNLVYFP
                     DLLAQGFEPHMPKEVWCSLTNQPNTSVDVTDTWDIKMQAILEHKTQVQDPKALIERFK
                     TRRTEDSTEENPRYEEKFKVVKYG"
     gene            10113..10679
                     /locus_tag="PLE39_01195"
     CDS             10113..10679
                     /locus_tag="PLE39_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE66965.1"
                     /translation="MNIVNVGYQSTNYYLIDILGGKLLVDCGWPGTLPQFSAELKRKG
                     IRLQEIKYLLVTHFHPDHAGMTQELKAIGIKHILLDVQVDFVFQMTEFFKTKPHPYRP
                     ITTDDSIRLKVEESREFLLKLGLSGEIIHTPGHSDDSVSLFLDEGFVFTGDLSPIFVL
                     PESDTISRASWDRIYQHHIKTVFPGHGK"
     gene            10693..11883
                     /locus_tag="PLE39_01200"
     CDS             10693..11883
                     /locus_tag="PLE39_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNE66966.1"
                     /translation="MTDFLNEARSLFSYTQTLRRDFHLHPELGFREIRTSGIVAKELE
                     ALGLEVTKGVGKTGVVGLLEGAKPGPTLLLRFDMDALPIHEETGAEYASQNAGVMHAC
                     GHDGHTAIGLTVAKLLNQHKDKLAGTIKFCFQPSEEGNNGEEVGGAEMMMRDGVLDGP
                     KVDMTLSLHLWNEKPLGWVNVAKGPVMAGAEEFKIKVIGKGGHGAIPDLAVDPVVCAA
                     QIVSASQSIVSRNVGPLETAVISFTVINGGTAFNVIPQEVMMQGTIRTFDPQVRMKVL
                     QRFDDVVRGVASAMGCQVELNVKQLTPALINAERVAASVQATAQRLLPNLHHDLSPYL
                     TMGAEDMAYMQAKVDGCYFFIGSNNHERHLDYGHHHPKFDFDEQALITGSALMAAAAA
                     DILK"
     gene            11926..12807
                     /locus_tag="PLE39_01205"
     CDS             11926..12807
                     /locus_tag="PLE39_01205"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNE66967.1"
                     /translation="MKKLTITILLLMLLLSACTPPVSPTEAPLATEEPSQVGPAVDIP
                     ANPEPLSTATEAPVAREAKISEVQNEVLARTLSTSDLAPASVGTSILPTGGVETGTDG
                     RARLDLLPEGTIVRVGPNSSFTVPEIVQENGQPKTVIELLFGKVYILLNGGSLEVETP
                     SGVASVRGSLMRVEYDAETKRIKASCLEGHCALKNENGDEVELVAGEYTYIEGNFPPI
                     DPLLIDSSEIEEWLAEIPELGDFLNPLPDPKDYPDVEDGNDNGNGSNDNGNGNDNNNN
                     DDDDDDDDDNNNGGYPN"
     gene            12967..13563
                     /locus_tag="PLE39_01210"
     CDS             12967..13563
                     /locus_tag="PLE39_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66968.1"
                     /translation="MTKFQKWITTALVVSVAAGVFTLQNNLQPPSSENVQSTDSTLTP
                     EIAAPVESLTPTLAYEGCGFMWATHDAPELTEKIDLAVRKMNDKAWANANFFGEDCVY
                     ADGRTTFGVMETDVFIHLPVDDLLDNEFFGDWIAQVMAMIVQIPKEEFQNRYGFVEFW
                     FEASETDHLVVRVPIQKYLEVGQGVSGVEMLQLFIETP"
     gene            13686..14216
                     /locus_tag="PLE39_01215"
     CDS             13686..14216
                     /locus_tag="PLE39_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE66969.1"
                     /translation="MPIKLSQLKQDFTEGLSQISRFWGFPKGMGAIFAVLYLSQNPLS
                     LDEIVEATGLTKGAVSTEVRTLARMGLVHRSSKLADRKDYYMAEDDFYKSIRSILKER
                     QNNEFDSAIRSVGDALSKLEAGDVTGDKAEVDFTYKRIQALQEFFNAIDSLTRAVVKL
                     ESLGFTNVKTILSVLK"
     gene            14226..15128
                     /locus_tag="PLE39_01220"
     CDS             14226..15128
                     /locus_tag="PLE39_01220"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNE66970.1"
                     /translation="MKIAVTGAFSYSGKYIAGRLLERGEEVFTLTNHPDRPDPFDGKV
                     KTFPLDFNDQEGLIRNLSGMDVLVNTYWIRFDKGDNTQPRAVENTKILVEAAAKAGVK
                     RIVHISITNPSAKSHLPYFWGKAANEKAVMESGMSYAILRPTVLFGKEDILINNIAWL
                     LRRLPVFGLPGDGSYKLSPVYVDDLAEMAVDAVYKKENYIWDAVGPDEFTFKEMVELI
                     GKSIGVKKLLLPFPPRLALLAAQFMSLFVKDVMLTPEEVDGLMANLLVSKEAPRCRTH
                     LSDWLERHRASVGSQYASELARHF"
     gene            complement(15161..15742)
                     /locus_tag="PLE39_01225"
     CDS             complement(15161..15742)
                     /locus_tag="PLE39_01225"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HNE66971.1"
                     /translation="MSNTERLPYRLREPFLATTELALFRALQQMVGARYLVCPKVALN
                     DIFYIVRPNENVHFFNKFFRKHVDFLLCDPQTLAPAFGVEIVRPIAKNETRESDRFIE
                     ELFIDAGLPLVHVPSSETYDPSDIVSLFQLAVTKVGGTAALRLDTSSDSVPLCPVCGK
                     MMVLRINRDGPRSGKKYYGCMDSPRCAGLVPID"
     gene            complement(15792..16559)
                     /locus_tag="PLE39_01230"
     CDS             complement(15792..16559)
                     /locus_tag="PLE39_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNE66972.1"
                     /translation="MNLQPYLDFATSLAYRAGRITMGYYNTGVRADMKENNEPVTIAD
                     HEAEKFIRAEIEKNYPGHAIIGEEFGHSQGNGSPFRWTIDPIDGTKSFIRSVPLYGVL
                     IGLEIEGRISVGAAYYPPLDEMLCAADGLGCWWNSRRAHVSSVDEISKACLVTSDFER
                     LEEKDPTLRERFARKKALLRTWGDAYGYMLVATGRAEVCIDPFLDIWDYGPYPVILRE
                     AGGYFGTWSGEEGHTPGDALACNAALKQEVITLMQGS"
     gene            complement(16556..17800)
                     /locus_tag="PLE39_01235"
     CDS             complement(16556..17800)
                     /locus_tag="PLE39_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66973.1"
                     /translation="MKLDLENERILSNRQLTLLRDISSLSAELMIPCHLVGGSVRDLL
                     LEQPIHDLDIVFEGDAISFGNILVNRFGGRLTAHQKFHTAIWHVPESLAEQIQTPFLD
                     LITARSETYARPGLLPNVTPSNMEDDLRRRDFTINAMAIRLDGEHFGELLDPLNGQQD
                     LKQGIIRVLHPRSFIDDPTRIFRAIRYESRYSFQLDPATRELVNAEALDVFRMLSGER
                     LRHELDLIFEEEKALTMLQRLSEFDALRYIHPKLPAFNPTYADHLEMETGLEVPASRT
                     TLGYMFWLLDLSEDDIVSISRRLEFTSELTHSVWAASILRKSLPFLIGSSPSVWASAL
                     ENLPLLAVYAVYLETGEGALLSYISFWRHVKARTTGNDLKALGLPPGPRYGEILTQLR
                     AAWLDGKITTEKEEKELLNTLL"
     gene            complement(17823..19211)
                     /locus_tag="PLE39_01240"
     CDS             complement(17823..19211)
                     /locus_tag="PLE39_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNE66974.1"
                     /translation="MRIVRYFFSRWQNWLGLLIVLMFTVVAISAPLLSPPDKNTQGSF
                     KKVGRSSDRTPHPPSETSILGTLPGQIDVYHTLVWGAREAMQFGLSVAIGAFIFGTLF
                     GSVAGYFGGFVNNIMMRIADAFLTFPVVAGVVLLQQLVAVTIESLGGIYWLNNDYFGR
                     VIDFQFTPPPFAVFLMKVDPILISLIIFSWMPVARIVNTMVLTLKNKEFIQSSRALGA
                     SPAWIIFRHLIPNSVGPAVVLAARDVGSSVILQATITFVGLGGASAWGILLSIGRNWI
                     IGPGGGLLRYWWVFLPVTIAIVLFGVGWNLLGDGLLEVLDPAANAYGGTSMIKRKQAK
                     GKKEAQSASDSKSIHHDSDSVAAQTATQGISVTLQNNPPVSHSQEKHPVLQVAREAIA
                     KKDFEHAIHAYSHLIKHRRNLNEIRQDMIEYVREHANEAVIWTLLGDVLTQEGKMEYA
                     EKAYEHARNLTQ"
     gene            complement(19208..20272)
                     /locus_tag="PLE39_01245"
     CDS             complement(19208..20272)
                     /locus_tag="PLE39_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE66975.1"
                     /translation="MPPILQFLIRRIFSAFISLIIITMVLYSGFMLTPPEARARIYLP
                     PGKGGERASEQYIENIIKENHLREPYLVQYFYWAKSLLQGSWGYSPTLNADVLPSLLH
                     RTPATLELALYSLLMLIPLGLASGLVSGWRPGSMFDSGFRGLAFLGTSTPSFIVSIIL
                     ISILYIQLDWLAPGRIDVKTELQMFRSGFVEYTGALTLDSLLNKRFDIFIIALKHLIM
                     PVFTLSIYHWATLGRITRASIMNERGKEYIVAARARGVKESKLIWKHALRAILAPSLT
                     AMALSAASIVTGLFVTEIIYGLTGISQIIVIAMSSYPDAPAALGFSVYSVIMVLGLML
                     ILDFIQATLDPRVREEVLKA"
     assembly_gap    20576..20675
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20831..21361
                     /locus_tag="PLE39_01250"
     CDS             20831..21361
                     /locus_tag="PLE39_01250"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HNE66976.1"
                     /translation="MNITVFGGAQPKEGSQAYEEARELGSLLAQRGHAVLTGGYMGTM
                     EAVSRGAHEAGGHVIGVTCIDIEEWRNSKPNQWVKEERRRQTLMERLMGLIEGCDAAM
                     ALSGGAGTLTEISLMWNLMIVESLPPRPLILVGSGWQSTFDQFFREFESYMPKHQREL
                     LYFAKDVKTAVTILQS"
     gene            21431..22870
                     /gene="proS"
                     /locus_tag="PLE39_01255"
     CDS             21431..22870
                     /gene="proS"
                     /locus_tag="PLE39_01255"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNE66977.1"
                     /translation="MAEEKLTTRAEDFSEWYNQLVIRADMADYAPVRGCMVVKPYGWA
                     LWENITSWLDAEFKKTGHVNAAFPTLIPMSFFEKEKDHVEGFAPELAVVTHGGGEELE
                     EKLAVRPTSETIIGHMYSKWVKSWRDLPILIVQWGSVLRWELRTKLFLRTAEFYWHEG
                     HTAHSSEAEAKEEMYRILDIYERFCLEEAAIPVFKGTKSDTERFAGAQITTSIEAMMW
                     DKRALQSGTSHYFGDNFAKAFEIQYLDKNNKLTYAQTTSWAISSRIIGAMIMVHGDDN
                     GLVLPPRLAPIQAVVVPIFKSDEEKAKVLEVADRIFAELKAAGVRVKFDDRDNVSSGF
                     KFNDWEMRGVPVRVEIGPKDVEKGTVALARRDRPGKEGKAFVSQANLDSTVKGLLDEI
                     QASLLKRATEYRDANIHNVTTFDELKEVVKDGWAFAYWCESRECEAKAKEETKATTRN
                     IPFDQPNEEGACVVCGKPSKRKVYFAKAY"
     gene            22957..23943
                     /locus_tag="PLE39_01260"
     CDS             22957..23943
                     /locus_tag="PLE39_01260"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNE66978.1"
                     /translation="MPAIDLARLRKQANRLADFFFLPDEFMKHLREILDFYVNYTLRT
                     KENVAPGSNLKTYRTPPAVLTQIENELRAVATANPDHALNLADTLWDEGALETRLLAA
                     FLLGRIPPQEERLLPRLTAWTQQIRDPDVRSALLSTSLARMRRENPNQFFNLIREYLH
                     PARTRTWSNGIQALIPMIADTTTANLPPIFDLVEPIIEEAPSTLQNDLAELIVVLYRA
                     SSNETTFMLKHILANTENPMTVVTLRRISSAFPPPLQSALRDLLRPQPLSKPSPVEDA
                     ESSDFVTEETSEPMVKKPRRKLTTREPKPESAATDKKTVTRRKKKDESASNG"
     gene            23936..>24405
                     /locus_tag="PLE39_01265"
     CDS             23936..>24405
                     /locus_tag="PLE39_01265"
                     /inference="COORDINATES: protein motif:HMM:NF017538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE66979.1"
                     /translation="MDNSKIIYLHGLESTSQSGKARQFAQLFPGMITPDFTGSFEERM
                     AQLAPILGDKKDWTIIGSSFGGLMGTVFTCEHPSQVRKLILLAPALLRDHFASYLDLE
                     PVSVPTVIIHGKEDDIVPLEPVRILAEKTFSNLQYILVEDGHRLHKAFDELNWK"
     assembly_gap    24406..24505
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            24515..25795
                     /locus_tag="PLE39_01270"
     CDS             24515..25795
                     /locus_tag="PLE39_01270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE66980.1"
                     /translation="MKQHIYWHTTVQMPDASNLTPIPEKADVAIIGGGYTGLSAARTF
                     AKNNVHAVVLEAETIGWGASSRNGGMTLTGLKVAMQTAITRYGRDLARSLFQASIDSV
                     SLVEQIVQEENINCGFARTGHLLAANKPKHYEALKGEVDFMAKEFNHKVHLVSPKDLH
                     TEIGTDVYHGALVDEVSAGLNPAQYVAGLAAAAAKAGAILCARARVNRIRRNGTRFIV
                     ETERGSLEVDSVLVATSGYTGNVTRNLQRKIIPIGSFIIATERLSNELANELSPKNRM
                     IFDFKHFLNYFRLWDNRMIFGGRAAFFPENENTVAQSGEILRNEMLKVYPQLKDAKVE
                     YVWGGTLDFAFDQMTHVGEEDGMHYSLGYAGHGVAMATYLGKTTAEAMLKGNIKEHPF
                     AKFAFPAAPLGLYTGSPWFLPFAGMWYKILDWVE"
     gene            complement(25806..27164)
                     /locus_tag="PLE39_01275"
     CDS             complement(25806..27164)
                     /locus_tag="PLE39_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE66981.1"
                     /translation="MKKLFGRLSKAPQKDEAHTQTSFGVGTKIHQRYRLDAEIGRGGM
                     GIIYRAHDILNDREVAVKIINAATTNALSLDQFKREMEISTKLQHPHIVTTHEAGTVD
                     TDGKSVPFLVMELVQGTSLDKAGNLTYARIIDIARQVCDALAYIHDQGFVYRDLKPSN
                     IILEPRGFQTFVKLLDFGLARPRGEAYLPNESSLAGTVFYLAPELVNGEPADVGSDLY
                     AFGAVLYEMITGRVPFSNVDEQTILTQHLNEQVALPSQSRADVPSALESIVMRLLAKT
                     PQERFSSAQEVGFALERISIAKTAATIGNLPDAIPAAPQEPVTELIDLLTKSRLVTLL
                     SNDETPTLAAASQTKDIFSDGIWLIQLTSIHDPARVMPTVMEVLNVSEIPNRPPVIAL
                     IEFLREKHLLLLLSHCGHLTGACVQLIKTILPACPEVRIVAVSTQALNLPEEICYKGT
                     AH"
     gene            27252..27986
                     /locus_tag="PLE39_01280"
     CDS             27252..27986
                     /locus_tag="PLE39_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNE66982.1"
                     /translation="MRILVISDIHANFTAFEAVLKDAGSFDAVWCLGDVVGYGPEPNE
                     CINRLREFNPVCLAGNHDMAVAGRAAVWDFSKDAQEIIFWTRHRLSTQNLDWLNTLTD
                     RPLMTDFGITLVHGSPRDPVWEYMTDTGIAKSSFELIETPIGLNGHTHFPVIFRKPWD
                     DWRVLEEYPRINSPLKLTLDRMWINPGSVGQPRDEDPRAAYAMIDTEEMTYVYRRVLY
                     DVSAVQKLMKQEKFSNRLIRRLRFGQ"
     gene            28004..28441
                     /locus_tag="PLE39_01285"
     CDS             28004..28441
                     /locus_tag="PLE39_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNE66983.1"
                     /translation="MYRKILVPLDGSKIAEGVLPHAKSLAYSEGAELILLTVGANPAL
                     DFAFSDPGLAQTAVDEQEEKSKAYISRIESELKADGFKTSTLLRVGSVAEVILGVAEE
                     LQVDVIAMSTHGRTGPARWLLGSIAERVVHNSNVPVLLIRARG"
     gene            28870..30273
                     /locus_tag="PLE39_01290"
     CDS             28870..30273
                     /locus_tag="PLE39_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE66984.1"
                     /translation="MSQVTANTPASGEQVEQGLPRIIFASAAGTMIEWYDFYIFGSLA
                     TVISSKFYQTGTPLGDLIAWLAAFAVGFIVRPFGAIVFGRVGDLIGRKYTFLVTMTLM
                     GLCTFAVGLLPTAETIGPAAGIILIALRILQGLALGGEYGGAATYVAEHAPNGRRGFM
                     TSWIQITATLGLFVSLGVILTTRSIMGEEAFAATGWRVPFLISILLVGLSLWIRYSLR
                     ESPLYVKLKEKKQVSKNPLKESFGNPYNLSFVILALFGATMGQGVVWYTGQFYALFYL
                     QKIFGVSLVDSNLIVGAALLAGTPLFVVVGHLSDKYGRKPFMMAGMILAVLTYLPIYQ
                     TMAKYAPFVGNAADKVVNPGYSPFMLGVLVFVQVVYVTLVYGPIAAFLVELFPTKIRY
                     TSMSLPYHIGNGVFGGLVPIIGLSLIQSTGNNFAGLWYPMVVAGICAVIGLWKLPETN
                     HIDISSDASVASLGANK"
     gene            30363..32276
                     /locus_tag="PLE39_01295"
     CDS             30363..32276
                     /locus_tag="PLE39_01295"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNE66985.1"
                     /translation="MNFISPTVNRWLQEAKENPEAFWERAAKELHWFKTWDKVFEWNY
                     PNFKWFSGALTNLAYNALDHHVKRGWGGHTALVYMNERGERQLFTYAHMLYEVKRMAA
                     ALRGMGIKKGDRLTIYMPTCPEAIMLMLATVRIGAIHSVVFAGFGENALGDRIQASGS
                     RLVFTTDITYRKGKDVPLKGIVDGALAGTKTDVEHVILLKRGNDDVSMQDGRDITWQE
                     FIKYAEGQNGDCTPMEANEPAFILATSGTTAKPKLAIHTHGGYQVHIFSMARWVYGLK
                     PTDVWWATSDIGWIVGHSYIVYAPLITGCTTLAFEGALDHPHAEANWRIAIEEFAVSG
                     VFTSPTAVRLLMRYGDEPLSRVNHDYLERIFCAGEVLNAPAWDWLQNKMLGNKIPVID
                     HMWQTETGGPVFGNPYGIGMLPTKAGSATIPLPGIEVAVLTPEGEPCAPNEKGIMVIK
                     RPFPGLTPALWGEPERYGKDYWSKIPNVYYTGDSAHIDEDGYVWFAGRADEIIKIAGH
                     RIGTIEVETAFLKHPAVAESGVIGHPDELRGEVISAFVLLKNGKTPSAELKKELIDTV
                     RHELGPIAVIGELNFVSMLPKTRSGKIMRRVLKAVTLDKDPGDITTIEDEGSVTEARQ
                     AWQQMKDDISKGV"
     gene            32371..34266
                     /gene="acs"
                     /locus_tag="PLE39_01300"
     CDS             32371..34266
                     /gene="acs"
                     /locus_tag="PLE39_01300"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNE66986.1"
                     /translation="MAKKEMEGEVYYPSEDVIAQARLKDWDALAEKAGKDLQGFWADE
                     ASELEWFQKWDKVLDDSNKPFFKWFVGAKTNIVHNAVDRHLKTHRKNQLALIWESEDG
                     KQERTFSYYAMNREVSRMANIIKSMGVQKGERVTIYMGRVPEIVFAMLACAKIGAIHS
                     VVFGGFSVDALQGRIDDSQSKLVITCDGSYQNGKIVELKNIVDESIKRCPSVENVIVV
                     KRTGQPVAMEAGRDHWYHDLCALPIANGKCATEVLDAEDPLFILYTSGSTGKPKAILH
                     THGGYMVGTYSTIKYVFDVKDEDRWWCTADPGWITGHSYIVYGPMIAGATSMLFEGGP
                     TFPYPNRWWQVVERFGITIFYTAPTAIRGLMRFGEAWPNKHDLSSLRLLGSVGEPINP
                     EAWKWYYRVIGKEKCPIIDTWWQTETGAFQITPTPVVPLKPGSGTRPFFGQKAEIVDE
                     QGNPVPDDTEGYLTLLNPWPAMLRTIYGDDERYVNQYWSKYPGRYTTGDSAKRDKDGY
                     FWIIGRVDDVIKVSGHRLGTAEVESALVSHPAVAEAAAIGLSHEVKGQAIHTFVLLRS
                     GFSPSPELAEELRQHVAKHMGPIARPEDVKFLDKLPKTRSGKIMRRVLKARAQGLPEG
                     DISTLDE"
     gene            complement(34332..36023)
                     /locus_tag="PLE39_01305"
     CDS             complement(34332..36023)
                     /locus_tag="PLE39_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE66987.1"
                     /translation="MAGNGLIGKKVDRYEILEKIGEGGMATVYKAYDTRLEREVAIKF
                     TKREAFPPDQLEMILKRFEREAKALGRLSHPNIVGVIDYGEYDGAPYLVMTYLPGGTL
                     KQKLGKPIPWREAVETIIPIARALHYVHSHNIMNRDVKPSNILLTENGEAMLTDFGLV
                     KLFEGQDTSQQLTDSGVGIGTPDYMAPEQWTGETTALSDMYSLGVVLYEMITGRRPYI
                     SDTPAGILLQQVNEPLPLPKTYIPDLPANIEAVLLKTLARNPKDRYANMNAFANDLQS
                     ILTGGQVTATAVATDILREQMTGKVSKKEIQEVKTQIEQKQAEQKQAEVPQRERTSPT
                     SISNAGQPKSSKPMVSIALLFLGAVGLLVLCGGCWLAYSFGPWAREAETPTALAVTSP
                     EVPTATLEIEDTPTAKPSDTPIPQVTPTNTAVPILLPQQWNGTYRQAGFNSTPISIVI
                     EKMNGQTFTGKMVWTGGTNYRGAITVIEGEFVTDFGDATEQAKWNKHLDYQNGDRDGI
                     WIKWTETKFVSGGGYTLGGWYYGHVGKDGSMTGIYFLNDQASSYANDSWKLKLVK"
     gene            complement(36190..37752)
                     /gene="ftcD"
                     /locus_tag="PLE39_01310"
     CDS             complement(36190..37752)
                     /gene="ftcD"
                     /locus_tag="PLE39_01310"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNE66988.1"
                     /translation="MTNLLIECIPNFSEARRPEIIDQIVAAIQSVSEVKLLDRSSDLD
                     HNRTVLTFAGSPAGVEEAAFRAIKTASELIDLDQHTGAHPRIGATDVCPFVPLGGANM
                     ADCIAIAQRLGQRVGSELSIPVYLYEAAATRPERTNLENIRKGQYEGLKTEIETDPNR
                     APDFGPAKLGKAGATVIGARNPLIAFNVYLTTEDVEIAKKIAKAVRQSSGGLRYVKGL
                     GLLVDGRAQVSMNLTNFRETPIARVVEFIRREAQRYGVGIHHSELVGLIPEEALVDAA
                     VWYTQLDQFDKEQILESRLFDSGPQTTGSSSSPSHGLSTPQPASFIEQLAAPTPTPGG
                     GSAGAYAGAMGAGLVAMVAGLTIGKKKYADVEAEMQAIRVVAENLRQELTQAVDDDAA
                     SFEVLMATFKLPKDTDEQKATRNAAIAQATLNAAHIPLHVSEDAVKVMGLALKCAKHG
                     NVNAISDSMSGFAMARASITAAGCNVRININSLEDRTLGEKMLDELAELEKEADKLEA
                     EIRAVAKERGGI"
     gene            37848..38711
                     /locus_tag="PLE39_01315"
     CDS             37848..38711
                     /locus_tag="PLE39_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE66989.1"
                     /translation="MADNLPEHLNKLSPREIEVVDHVLQGKSNKQIALQLGITERTVE
                     FHLKNVYTKLGIGSRMELAVKLWKSADDEVKASLRESTVAESVEIAENGNQPMWAASV
                     KRMLSVLKKEFAMTMKTILNDLGNAVQKHLLAFSLFYFIAAGLTTHYLVVGFGLYFIP
                     SYLLLGLVLGMSSLYLGLRWHGIKSGEVHVRPIWAVGFLALPFLAGLLDLTLLATLAK
                     AMGEVTVNLIGLSSRAVWMVADNGSPFLLLSRSITLDDIWLYGPVLYMSLLALAGRFY
                     TIRILKRTDAV"
     gene            38838..39440
                     /locus_tag="PLE39_01320"
     CDS             38838..39440
                     /locus_tag="PLE39_01320"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNE66990.1"
                     /translation="MEKSRLEAFSDGVFAIVITLLILDIRFPEVEYAQFSETLVSVVP
                     RILAYIMSFVIIGLYWVIHHNSMHAIKKTDRGFLWLNILLLLCVSFIPFPTSLVGRYP
                     FQAGPIVFYGFTLIACNAVGYIMLLYVYYHPHLAVAEFSKQYMRSHTPNYVIVNGAYL
                     GAILLANSFPLVSYLIYIAVVILLIVFLPKLDDGIKYHVE"
     gene            39634..39864
                     /locus_tag="PLE39_01325"
     CDS             39634..39864
                     /locus_tag="PLE39_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66991.1"
                     /translation="MRAFLIQSVVALVFGFVLGVIGLLVGAWFGGNFAASFEFNGVRG
                     YEATGQVGFILFALAGLIAGWKFMEKRTKKLR"
     gene            39876..40508
                     /locus_tag="PLE39_01330"
     CDS             39876..40508
                     /locus_tag="PLE39_01330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNE66992.1"
                     /translation="MSLILFDFDGVLADTLGDLLQFGQEVCDELGVQHIATQEDLNSL
                     EVMSFATYGIQLEVPAHLVDEFVRRCLSRFAEKKSPPAIFDGLAEVVRKLAKENVLAV
                     VTGNSTQNVNAFLAEHGILDCFRAVYGVDLPGSKVQKISLAREQFSAGMMRESVFMVG
                     DSLSDIRAAKEAGIQSIAVAWGHQSLDRLLSGDPDHVVRSVQELLTLFAV"
     gene            40519..41175
                     /locus_tag="PLE39_01335"
     CDS             40519..41175
                     /locus_tag="PLE39_01335"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNE66993.1"
                     /translation="MNTNSFDLVIFDCDGVLVDSEPLANKVYVQMLAELGHHLDYDAT
                     LREFSGAAVFKRIEATSKKLNWTPPVNFLPEFFSRLSELSLRELKPVDGIRQLVESLT
                     VPVCVASNGARDEIVLRLKLANLTEFFGNAIFSGLEVPHPKPAPDVFLAAAQAFHVPP
                     SRCIVIEDSVPGVTAAVRAGMRVYGHAAITPVEVLREAGAMPFRDMRELKRILSAQGV
                     "
     gene            41291..42352
                     /locus_tag="PLE39_01340"
     CDS             41291..42352
                     /locus_tag="PLE39_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNE66994.1"
                     /translation="MTLPPEEVILKGAAAWNKWQEENKFPPHFARPSWYECRDERGMQ
                     IKGRNELDFSGIHLTNASVHLAFAEGLNMRNAEVVGCHFEEGDFSRADFSNTTFVNTK
                     FNKTILTDAVFEGATFINCNLNRVNLVNADFRLKEIRETVVYGISAWDLKTDPAMKQS
                     RLVIEKSYELYSDLIASGKIPMMVDDIEMAQFVYYLSSHKNMRNMINILNSKGVLLLG
                     KFKDGGLERLYSLMDWFKTRNYTPMLFDFERPNSLDYTETVITMAGLAKFIVADLSGG
                     SVPQELYATLSSFQKPVIVYSDQPAYSMFKDIKRKNRFSFEIVFSDEKDLYAKMEHSV
                     KEAELGHAQLAHDLIDSHG"
     gene            42478..43266
                     /locus_tag="PLE39_01345"
     CDS             42478..43266
                     /locus_tag="PLE39_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE66995.1"
                     /translation="MRIYKYWAVERQTFEVQGEQKTGTFYGGSNESVEDARAKVQAKL
                     DRVKRKIAGDPRAFNGYEVDIREQVLEEINPQAVITRNRYGAKVLNVASMMILDIDKP
                     KLSLSGLFSKKDDKSKMYEMVEKLASSKYSQFGFRIYETFQGVRVIVLGRDFDPRNAV
                     TTAMMAEFNCDPLYTILCSKQECFRARLTPKPRRMGMKSMTIRFPREDTDLQTENWLS
                     EYDRRSGDFSVCRFIGQAGAAAPTNDLVHLHDELTGASIGRQLA"
     gene            43281..43610
                     /locus_tag="PLE39_01350"
     CDS             43281..43610
                     /locus_tag="PLE39_01350"
                     /inference="COORDINATES: protein motif:HMM:NF038084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HNE66996.1"
                     /translation="MNMQDIPFGTTNWGEIAPTEHKGITGMALWRTQQFGNIRVRMVE
                     YSAGYHADHWCEKGHILLCLEGELHTELADGRKFTLTPGTSYQVSDGTDPHRSFTETG
                     ARLFIVD"
     gene            complement(43639..44733)
                     /locus_tag="PLE39_01355"
     CDS             complement(43639..44733)
                     /locus_tag="PLE39_01355"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNE66997.1"
                     /translation="MEKILILGGYGYTGKLLAKYLLAQTNVQIMIAGRSLGKAKHFTS
                     ELNNSRATAIQVDAASFDSLLQALQGITLCLVAAPTTHHAETVVRACIQARVDYLDVQ
                     YSSRKLEVLFAAEKEIEQAGLCFVTEAGFHPGLPSALIRYASKKLDVLESALIGGYLN
                     MKELPYTEAVDELMEGFINYQAQVYKNGSWTKPAAWDSRPFHFGDGIGKRTAYSMFFE
                     ELRGIPKLYPSLKETGFYIAGSNWLSDLVITPIIFVGLKLAPKRGLHPLGKLMWWSMG
                     KSKPPYVVALKAEAKGLLNGRQVEVHVRIAHPDGYEFTAIPVVAFLKQYLDGSVRRDG
                     VHMMGHIAEPLRLFKDMQEMGVELNENITD"
     gene            complement(44739..45221)
                     /locus_tag="PLE39_01360"
     CDS             complement(44739..45221)
                     /locus_tag="PLE39_01360"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNE66998.1"
                     /translation="MGMLKNKPDQLQKFLFKVPVWLHKMGLGGWERLIGAQWMLITTI
                     GRKTGKRRDVMVDVMDYNRNTDTYYIEAAYGAHADWYKNIQSNPIFEARVGRRKFKAQ
                     AGALSTEGAGEMLVQFYRAKPAYTRSVMAMAGMKFKDEEELRLLGKNLTLLAVKPEKE
                     "
     gene            complement(45225..45992)
                     /locus_tag="PLE39_01365"
     CDS             complement(45225..45992)
                     /locus_tag="PLE39_01365"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNE66999.1"
                     /translation="MKLKGLDSLSKHVPELNSTSGRTRIVLYVVSLLALVTAYFIVTD
                     NIPTWSIDSEIIIASLGFLILSLFFSRKPVYQEKYKDLAYRNAFAHYALPGLCLIFGA
                     VAHAGYMNGPVIPTGWWTTVFTVLGWYWVLVGTILWIRSIATFGVDNLNLLYVYHPEE
                     SRIVDSSIYAILRHPIYASAIRVWVGLALLNGNGNSVIFSVFVPLGLTGWIRLVEEKE
                     LLERFGKPYLDYRKHVPAFWAKPRDIGKFFKFLITGN"
     gene            complement(46136..46741)
                     /gene="cyaB"
                     /locus_tag="PLE39_01370"
     CDS             complement(46136..46741)
                     /gene="cyaB"
                     /locus_tag="PLE39_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HNE67000.1"
                     /translation="MGEQETEVKFFVRDLKKVELRLLELKAQLIQPRVHEINFRYDLP
                     DGSMRTNGRVLRIRQDAVATITYKGPSDLVDGVFSRQELETQIGDFETAHKILIALGY
                     VKILTYEKFRAVYEIDGYHIMLDELPYGDFVEVEGANGAGIRAMSKRLELNIEAAVGA
                     GYARIFEDYNTRQGRASSDLTFDALRGKIPSPEELRIRAAD"
     gene            complement(46742..47239)
                     /locus_tag="PLE39_01375"
     CDS             complement(46742..47239)
                     /locus_tag="PLE39_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNE67001.1"
                     /translation="MKYITTVDDQQFEIEVVDERHIRIGGRLLQVDFESVSGQPVFSL
                     LLDGHSYESFVYQGEEDWDVLLRGRQYQVKVEDEREKRLRAAAGGGIAEGGEFHLKAP
                     MPGLVVAILVEEGQDVKKGQVLLILESMKMQNELKAPRDGTIGRIRVKAGESVEQKQA
                     MLSVH"
     gene            complement(47253..48761)
                     /gene="accC"
                     /locus_tag="PLE39_01380"
     CDS             complement(47253..48761)
                     /gene="accC"
                     /locus_tag="PLE39_01380"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNE67002.1"
                     /translation="MFKKVLVANRGEIAVRIIRACRELGLETVAVFSEADRNALHVRY
                     ADEAYLIGPAPSRESYLRADKIFDVAKKSGADAIHPGYGFLAEREDFAEKCAELGIKF
                     IGPKPSSIAAMGDKGQARATVIKAGVPVVPGTEDVGNMTDDDLLRIAPTIGFPLLIKA
                     TAGGGGKGMREVRSIEEMPTLLQSARREAESAFGDGNVYLEKLIEGARHIEFQIIADS
                     FGNVIHLGERECSIQRRHQKLLEEAPSSYLDDDLRTKMGNIAVKSAQAVDYINAGTIE
                     FLVDKDRNFYFLEMNTRLQVEHPITEMVTGVDIVAEQIRIARGRQLSYTQDQIKFNGH
                     AIECRINAEDPFNGFLPSTGRITHSLIPTGPGVRVDTGVYPGFEITPFYDPMIAKLIV
                     WGETRAQAILRMRRALEEYRIVGVRTNIPFHQTLMDSHRFMAGQFDTRFVEERFSMDD
                     AVEARASSAEIAAILATLVEHQQSERSAQVVRRNERDTSNWKWVGRWERMHR"
     gene            complement(48779..50326)
                     /locus_tag="PLE39_01385"
     CDS             complement(48779..50326)
                     /locus_tag="PLE39_01385"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNE67003.1"
                     /translation="MSETSKIQKLTELKAQSRLGGGQARIDAQHKKGRLTARERLDLL
                     LDKGSFREVDPFVVHRTHDFGLDEQKFMSDSVVTGWGTIEGRLVYVYSQDFTVFGGSL
                     GEVHAEKICKIMDMAMKNGAPMIGLNDSGGARIQEGVVALGGYADIFLRNTMASGVIP
                     QISAIMGPCAGGAVYSPALTDFIFMSRNTSYMFVTGPDVVKAVTHEEVTQEELGGAGV
                     HSEKSGVCHVAADSEADTLYLIRKLLGYLPQNNMEDAPFVQSDDPLRMDEALNSIIPD
                     DANKPYDIKEVIRMIMDSGQFYEIHENYAQNVVVGFARLGGHSVGIIANQPAVLAGVL
                     DIDASEKAARFVRFCDAFNIPIITFEDVPGFLPGTFQEHHGIIRSGAKLLYAYCEATV
                     PKLTVITRKAYGGAYCVMSSKHIRSDVNLAWPTAEIAVMGPDGAVNIIFRKELDAAAD
                     PVQKKAELVAEYKEKFANPYVAAQRGYIDDVIEPKETRPRLINALEMLSNKRDANPAK
                     KHGNIPL"
     gene            complement(50292..50708)
                     /locus_tag="PLE39_01390"
     CDS             complement(50292..50708)
                     /locus_tag="PLE39_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67004.1"
                     /translation="MKKFSFRATLLQMLVLTLTAFNLLRMWTALAWQDILTEFSTQPS
                     PIVLAVSGAVWALCGIVLLWGIWQKTAWAEKLLLGASAGYSLWYWTERLAWQNPHPNW
                     LFAVIVNLAIIVFILFSKKSLTREAYERNIENPKID"
     gene            complement(50804..52909)
                     /locus_tag="PLE39_01395"
     CDS             complement(50804..52909)
                     /locus_tag="PLE39_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67005.1"
                     /translation="MNFLKRLPSFEAVLIAVILGIHIYAAFSDAYNLPNSWFTRDDAY
                     YYFKVAQNITEGHGITFDGINLTNGYHPLWMLICIPIFTLARFDLILPLRVMLLVMAG
                     FNAATAILIYRLVKSNLSNAVAMLAASFWAFNFYIHNNVYEFGLETPLAAFAVVLFIY
                     KLSQLEKDWRTQAVTKRQWLNLAGVATLVMLSRLDLVFLAILGGFWIILRGKPMRFLL
                     PLDVVIIFLSMTTSVALRTGIALYNSMFAASAVQAVVLALAVKIIALYFFGAYQHPRE
                     NSVVKTLLQAGLALTFSTMALTGLYLLLAQLGIGRDFPRSAFLIDLGLSAFLILALRL
                     AGYWFGNRAINPQNNTDKKEKSIFNNAPLKEFQSNWKTWFTEAIPYYGIVGGALLLYM
                     LFNRLVFGTSSPVSGQVKRWWGTLGETVYDSPAPNWTAFFGLSFQGAYDAWQPASGLF
                     LWLVKVNFPKFADIPIVSDLSYTFLALLVAVAVITLFVNANRTRRAFSNMALLPLMAG
                     CGIQILAYTTTAYGGAKEWYWVSQMVFVTLLGSVVLDFLIRPLLRTKAQLAIQYVSAA
                     ACLLLAYRFGAQVVKIMPYNFFPPERPYMDVLPYLEENTPPGSIIGMTGGGNVGYFIH
                     DRTIVNMDGLINSYEYFHALQNGEAPAYLREHGMTIVFASPRLLELPPYFGQFNPYLE
                     SYSEYGGKGLLYLLDKPKY"
     gene            complement(52910..53941)
                     /locus_tag="PLE39_01400"
     CDS             complement(52910..53941)
                     /locus_tag="PLE39_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNE67006.1"
                     /translation="MKTILFHKHGGPEVLEYTDFPMPEPKNGEVLVRLKAAALNRMDV
                     MVRNGWPGLKLELPHINGADGAGEVAGLGENTKGFKIGERVVVNANLGCGDCEFCLKG
                     QDNMCLNWHLLGESVRGTYTEYICLPARQLYKLPEDFNLHQAAAAALVYQTAWHSMVK
                     RGGVKVGETVAIVGAGGGVNSASIQVAKYLGARVIVIGSNSKKLKEAEALGADILIDR
                     SKEEDWSKAVFIATQRRGVDAVVDNVGTTFPLSLRILRKGGRLLTVGNSGAPRFEIDN
                     RYIFAKHLSIIGSTMSTLADFAEVMDLVVDGRLQPIIDRTFPLKEAAAAQERLWNGEN
                     FGKITLDIP"
     gene            complement(53938..56133)
                     /locus_tag="PLE39_01405"
     CDS             complement(53938..56133)
                     /locus_tag="PLE39_01405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE67007.1"
                     /translation="MDIHSHQPIPRKYWLVFALILVVLGQALMREPQPFPYFDTLADT
                     LKNWNDDAYLALSNPFNTLIGLALILLGTIVSALSTRTAPAEDYPVMGTMHWEKWKRY
                     KHWVILNLIVYGFIMVQLALHKDSVTFFWGWLAFLLVFTILFWENEHKPMEWITLTDA
                     AWMLGLFTLATAMGAYLLNDLPAGWIADEGPFWVAARNIAIGKTDPSFFDTGVFTFPI
                     ASSLLQGWIMRWAGINMWGWRFASVLPAAGSVIPLYLLTRELFDRRTAIAASFMMIAN
                     PYFLAFARLGYNNIQALIPVTLCLYFLTLGLKKNSLYYFWLAGLAAGVGFYTYFAAWL
                     GLAAAILIILAFTLISRQSFRSGITSLLVVSTAACLVILPRVLYGASGENEKSLTYKI
                     WETGPINLFYGKLVFGDERMVEADIFNLEGVDLFYDASLYKLVFTRGFIRTTAVLFDP
                     IGYNDHPIVFGLAGPGSSIFFALGFGMALVDLKKLRHLIMLIFFLAGFFFLGVLASIP
                     PRPTHMVAILPAIAMISASGLTNFTTTVLNTIKERSIMLTTAGILSIIVLMGCVQYFF
                     LLPFAYAPPNQNDYIAWLTRQIQEPIHLYIIDNQVEPVQDSLSPHTTTMLSSAEISVN
                     PEQVSTWKNFVAFISPQSGADYAEELAAQIPGADVQAAYAPGQRLRGYVISDLPLKTS
                     MDISLQHGITDLWNSPARSILLICGVGSIFLFAMQWLTGKTSNKMSETL"
     gene            complement(56176..58233)
                     /locus_tag="PLE39_01410"
     CDS             complement(56176..58233)
                     /locus_tag="PLE39_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67008.1"
                     /translation="MNTLSNLTKSITRAWGRLLSSPTTWYTVLIEWSIIVCAAYFFTA
                     PVLLDFDPNQLQQTGEHNESSTRPLLAEIGLLQHGEIPLWNHYMQTGFPHAGDLLGHF
                     WSPIATIPVILWGGINGMKVSIFISFVLAGLGQWYLAQVSGLKGIFRLWSSLTFMFSG
                     GLAFLWRLGWYELLVGMVWFPWTFAAFWQALKKRDITSMAWTAFCVAMILFTGGGYYP
                     FYLIASAGTMLIVALIAAQPEERFIQIKRALSIAVFSAGLIAVMALPIYDGYRLIVRE
                     SGDDLVQQGSQPVSYAVMNYVISQGEWFNTEILGTAGGWNWFYTGPLMLGALLFLPLG
                     FQHSRKRSMLAAMLAISIVLLLWVANRYPPVKYLYDYVPFLYRLRFPNRLLILAASPL
                     LILSAASLQGLMIVLRKQFKNYRISFSYQNDGLEKTTRLRGLTIIIFIVILFFSLKDV
                     YLTNHSFGFVSFPIEPKSLQALRYLKNKDEGMYYINLGGGALYWSWTPAAYIVQLPVI
                     NFNYNQHLLNRDSQNLPEAPFVATPKYQFSPTLENPPGATLLKDFDGVGLWYFADALP
                     FAFTAPGNELVNGTKLDPTHITEASAHYDGPNRVVVTTAEDHTGDVVVALVNNYPGWK
                     VLRDGEPVPLTPANEYLAAPALPGKHTYTFIFDPPLHKIGLTITLLTIFALLFFVHRE
                     QLD"
     gene            complement(58233..60290)
                     /locus_tag="PLE39_01415"
     CDS             complement(58233..60290)
                     /locus_tag="PLE39_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67009.1"
                     /translation="MSSGQISLIAVIIITIYLDWVLMKWLKKRKKENVEKKPVQKWDI
                     QLSSFYKPILQWISKFNFELPLIALWALWVGRVYLNFDRNMVPGYGNDDFLLSIYPYF
                     PFIRVLQCGDCFFWNGLLNGGSPTFGDSIGALLHPVMAGIMLVFGVTNGIKVAMLASL
                     IMGGWSLWWLAKVLDLKMVTRLWVSALAVVGGHLAGPLFSGDVGIIFSTAAASLTVAP
                     AVALGLHGRRRDAILFGLMVAQAILSGQGYLQLALFAGVVPALLIFGINRYLQIGKIW
                     KELALALVIAILVSSVVLLPWFTFGSQISRPTEPRSDQPLEYMPMNLVVRDMNFFMTE
                     TLGKIAAPNLYVMYIGWIPILLAIAAWRFTPRENFRLYLFFAIAIALIFFLGSGAPLR
                     WAKDWLPGYLRDIRNPNFGVGLAVTLIFGLAAIGLDQILNLKWPVVSVYNNVTKKSHP
                     IDFRWLLAPILLWSITSVYEFNSQFRVLHEWPKELVRAAESIPTQPAQWVRPVYGNWT
                     YVAAAVRHNVKITETFRIWEMIGRPNPPMSLDSGNDPAMVNDPNYLKTVEGISFVLYP
                     ENFYAFVETDTGRIPCEAIADGGHIQVKCNNAETGDLVVYENALQGWRVWRDGKSTPL
                     EEGQWLKVSAPAGNHTYKFRYWPTDVLGGFVLTLIGLGLSYWFWQKEQPSPVTTSPER
                     ESF"
     gene            complement(60302..61471)
                     /locus_tag="PLE39_01420"
     CDS             complement(60302..61471)
                     /locus_tag="PLE39_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNE67010.1"
                     /translation="MTELPIHPLSDEHKMLRDAARDFAQKEIAPIAAEFDESGEFPYA
                     TIRKMGELGFMGIEIPEEYGGAGMDALAYVLALEEICKVDASHGVIMSVNNSLYCHGI
                     LKFGTEEQKKNFITPIATGKAIGAYSLTEPQSGSDAGAMKSRATRDGDFYILNGRKSW
                     VTSGPVANYFVVFMMTDPDKNQKGVTAFLVDGNTPGLTRGKKEPKLGIRASATSELIF
                     ENCRIPAENRLGEEGEGFKIAMTVLDAGRIGIATQALGIAESAYEAARQYSGQREAFG
                     QPIAAFQGTGFKIADMKTRIEASRLLIYNAAIAKENSKTNHGRYSLEASMAKLFASET
                     AMFVTHQAIQIHGGMGYSKELPVERYFRDAKITEIYEGTSEIQRLVISRMETGLR"
     gene            61701..>61880
                     /locus_tag="PLE39_01425"
     CDS             61701..>61880
                     /locus_tag="PLE39_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67011.1"
                     /translation="MKKLVNKILVLFVFASLVLSACGGTAATEAPAVEATEAPAEATE
                     APAATEAPAATEAPAA"
BASE COUNT        14923 a        14843 c        16417 g        15497 t
ORIGIN      
        1 ccagggagac tcaaagagta aaaaacttcg tgcttttgtg tcttcgaggc ttcgtggcag
       61 ggttttcaga cactctctta aacctctgta aaggttgatt gaatcatgcg atctttagcc
      121 gatatcccac accaggctcg gtgatgatgt gtctgggccg tgcagggtca gcttcgatct
      181 ttcttctcag gttgctcatg ttcacacgta ataaatgggc ctcgttttcg taggcatttc
      241 cccaaacggt gcgcaacaat tgatgatgtg tcatgaccct gccagcgtgt tgaacaagcg
      301 cgcgcaggat atcgtactcg ttgggggtca gtgatatttc ttcgccagcc acttttacaa
      361 tgcggtctga aagatttacc agcaggtctc cactctcgaa tactgcctgg gtttcaggag
      421 acgaagcatg ccgcatggca acacggatgc gtgccattag ttcgcctgcg ccgaatggtt
      481 tggttaaata atcatctgcg cctgcatcca gtgcggagat cttgtcagtt tcctgttcgc
      541 gtacggaaac aatgatgatc gggatctgcg tccattcacg caggcggcgg gttacttcca
      601 cgccatccat atcgggcaga ccaagatcga gaataacaag atcaggcttt tcggtcgcag
      661 tgacgcgaat ggcatcttct ccgtccacgg cttcaacaac ttcgtattca ttcccgagcg
      721 atgctttcaa aaacctgcgg atcggacgtt cgtcatccac gaccagaatg cgcgtcattt
      781 tgcctcctcc attggcaggg caatcgtaaa tacagaaccg ccaacggcgt tgtttttggc
      841 ccagatgcgc ccgccatgcg cctcaatgat gcctttgcag atggcgagcc caagccctgt
      901 gcctgtgaca ttttctggcc gttgcacgcg gtagaacttg tcaaataccc gttcaaggtc
      961 ttcatgggga attccaatgc cattgtctgt tactgaaatt tggatttcat ctttcacaag
     1021 atcggcattg atctcaagcg gtgagccttc tggagagtat ttcaaagcgt tgtcgatcag
     1081 attgttgagc acatggacga tcaacacaaa atcaagcggg accaaaggca gcgcgtcgct
     1141 tatctggacc tcgatcctgc gtctgtccaa acggctttcc atctggctga tggcggatcc
     1201 aattaactcc tgaatgtcgc tgggctcgca ctttactttc aatgcaccag cttcgaggcg
     1261 ggtcatatcc aacaagtttc ccacaaggcg atttaatcgg tcagcttctt cacgtgcggt
     1321 ttcaaccaaa ctgcgatgtg cgggagattc caattcgctg gcttgttctt ccagtgtggt
     1381 cagtgcgcct gtgatcgaaa cgagcggtgt ccgcaggtca tgtgaaatgg agttgagcaa
     1441 tgcgttttgc agtttctcag ccgattgcaa cagtttgatc tggcgggcct cttccgcaag
     1501 gcggacacgt tcaatggctt gtgcgcattg gttggcaaag gcttccatta agcggcgttg
     1561 cgcaggtgtg agatcgcgtt cgatggtgtt tggcttgcca actccaagta caccaaccac
     1621 gccgcgcgct gttttaagcg gcaggtagcg cagttgcgcg gctggcaggg tgttggtgtt
     1681 tcgccctgca gcctctccgt ggcgatagac ccaatctgcc acggccgctt cttcatcgct
     1741 taattcaagc cctgtactta gaatgtagct tgtcaggcgg ttttctgtgg gcagcaaaac
     1801 agccacggtg cggctgaata tcttttcgac atgtttttgt aaaacagcaa gtatggattc
     1861 gagttcagca gaaaccgcca ggtcacggct caggtcgtat agctcggcag tttccgcctc
     1921 gcgttgacgt gccgcctcgg cctgttcact ggcccgcgct gccaactgac tgatgaccat
     1981 ccccaccaaa aacagggcga tgaaggtgat gatgtattgc gtgtctgaaa ccgcaaaggt
     2041 gaaatatggg gtgacgaata gaaaatcaaa tgccagcaca cccagcaggg aggctaatac
     2101 ggcggggccg cgcccgagat acacagaggc cacaaccaca gccaatagat agaccatcac
     2161 caggttggtt ggctcgattc tgccgccgat cacaaatccg atgatcgtgg caaacccaac
     2221 cagaagcatg ctcaaaaaat atttatagag agggctgtgc aatttcagcg gattttcgtc
     2281 agcggggaat gcaggcacat ccatgcttgt gacgacgtaa atatcaatgt cgccgctttt
     2341 gtgaatcaat ttgtctacaa gcgaaccgcg taaaaattcc tgccagcgtg gccgcagggg
     2401 tttgccagca atgatctttg taatgttgtt tttatgggcg aagtcgatca ccgcctgtgc
     2461 agtggattcg atggagccgt tcgatgaaag tgtgtaggag cgtgcgccaa ggtcttctgc
     2521 caattgcagg atcttcgcca cgcgatcccg cttttcctgt gacatggccg caaattgcgg
     2581 ggtttccaca tacactgtga tccattccgc attcagttca tcggcaaggc ggcgggctga
     2641 gcggatcaag cgttcgctca atgggctggg gctgacgcat accaaaagcc gctctcccgc
     2701 cgcccatgga cccgaaatgg cacgtgtctg catgtacgag cgcatttggt catccacgcg
     2761 ttcagcggcg cggcgcaaag acatttcacg taaagcggta agattccctt tgcggaagaa
     2821 cttgtcaatt gcacggctgg cctgttcggg gatgtaaact ttgccttctt tcaatctggt
     2881 cagtaattcg tctggcggca ggtcaatgac ctcgatctcc gatgcttcgt caatgacacg
     2941 gtctggaacg gtttcgcgta cgatcacacc tgtaacctgt gcaacgatat cgttcaggct
     3001 ttcgagatgt tgaatattca acgtggtgta aacgtcaatc cctgcgttca agatctcttc
     3061 cacatcctga aagcgtttgg gatgccgtga gcctggcgca ttggtatggg cgaattcatc
     3121 cacaagcacc aaggcgggtt tgcgggccag tatcctgtcc acatccattt caggcagggt
     3181 cacattgtga tagttgacct gttttcgggg cagcacttcc aacccttcga caagttcatc
     3241 ggtatctgcc cgtttatgcg tttcaacaca accgatgacc acatccaccc cttgtgcttt
     3301 gcgttggtgt gcggcttcga gcatggcgta cgtctttccc acgcctgcag cataaccgag
     3361 gaacatcttg agtttgccgc ggccatgttc ttcaggttga atgtgtttca ataattcatc
     3421 aggatcaggg cgctgttgca tcaaaattat tgtatcccat ccagtgcgag atttagcatt
     3481 aatacattta cccgcggttc acccagaaag ccaaattgac ggccttctgt gttctgctcc
     3541 acgagtgatc ggacttccgc ttcgctcaac ccgcgggctg ctgccacacg atgaacctga
     3601 tacaaagccg cggcgacgct gatatgcggg tcgagtccac tccccgaagc agtgaccagg
     3661 tcaacgggaa tcggaagcgt gttttcaggg tcggcggttt gcagtgcggc gatccgtgct
     3721 tggattgcat ccatcaaggc tgggtttgtg ggtccgtaat ttgagcctga agaagccgcg
     3781 gcgttgtacc ctgcggcaga tggtcgtgcc caaaaatatt ttggatcgtc gaatgattgg
     3841 ccgatcaatt ccgagccata cacagtgcca ttgaccgata tgaggcttcc attggcttgc
     3901 tgcggaaaaa agacttgtgc cagcccagtg actagaaatg ggtagaccaa acctgtgagc
     3961 acagtgagca gagtcaggat cgtaaatgcg gggcgcagat gtgttttcat gctgtgaata
     4021 ctcctgaaag aaccgcaacc gcccaggcga tcgaagcaag gaatacccag ggacggaagt
     4081 tggttggaat gttgagtttg tctggggcga tggcggcggt aaacccaagg attgccgacg
     4141 tatagataaa gtatggcaga aggtgatggt ttcgtttcat ggaatcctca cgcaacaccg
     4201 atccagacca ggataaggtc gatcagtttg atgaagatga atggtgcgat caaaccaccg
     4261 agcccataga ccagcaggtt gtcacgcagc aactggtcgg ctggcttggc gcggtaggga
     4321 accccacgca gggcaattgg tatcagtgca atgatgatga gggcgttgaa gatcactgcc
     4381 gaaaggatgg cgctttgcgg ggtggcgagc ttcataaagt tcaaggcgcc aagtacgggg
     4441 taggtggagg cgaaggctgc tggaatgatc gcaaaatatt tgctcacatc gtttgcgatg
     4501 ctgaaggtgg tcaatgcgcc gcgggtcatt aagagttgtt tgccgatctc aacgatctcg
     4561 atcagttttg tggggttgct gtcgaggtca accatgttgg cggcttcccg tgcaggttgt
     4621 gtgcctgtgt tcatggcaac tgccacatcg gcctgtgcca atgcaggcgc atcattggtt
     4681 ccatcgcctg tcattgcaac cagccgcccg cctgattgat attcacggat gagtttgagt
     4741 ttggcttcgg gggtggcttg tgccagaaaa tcgtccacgc ctgcttcggc tgcaatggcc
     4801 gctgcggtga ggggattatc tcccgtgatc attactgttt taatgcccat tttgcgaagc
     4861 tgtgcaaagc gctccttgat tccgcctttg acaatatcct tcaagtagat gacgcccagc
     4921 gcctttgtgt tgcgcgtcac caccaaaggg gtgccgccca ttcgcgcaat cgaatcgact
     4981 gacgatctca ggtcggcagg aaatgggtgc ccgttgaaat ctgccattga aagcattgtg
     5041 tctggcgcac ccttgcggat ggaaatgccg tcaaaatcaa cgccgctcat gcgggtttga
     5101 gcagtgaatg ggacaaagtg catgcctgcg ggcgcgttca tcgtgcgtcc gcgcaggcca
     5161 aacttttcct ttgccagaac cacgatcgaa cggccttctg gagtttcatc tgccagagat
     5221 gcgagttggg cagcttctgc gagctcgttt gccatcacac ctttgacagg gatgaattca
     5281 actgcctgcc gattgccgag cgtgatcgtt cctgttttat caagcagcaa tacatcaaca
     5341 tcgcctgccg cttccacagc tctgcccgac attgcgatga cgttgcgttg gatcatgcga
     5401 tccatgccag cgatgccaat ggcagaaagc aaaccgccga tggttgtagg gattaagcac
     5461 acaagcaatg cgatcaacac cgtaatggtg atggctgttc ctttgcctgc cgcctctacg
     5521 ctgtaaatcg aataaggcag caaggtggcg cagaccacca ggaaaataag agtgaatcca
     5581 gccagtaaaa tattgagcgc aatctcgttt ggagtcttct gtcgttttgc gccctccacc
     5641 atgttgatca tacggtcgag gaatccctgc cccgggtcgg cgctgacgcg gacgatgagc
     5701 cagtctgaaa ggagacgtgt gccgcctgta acagcggaac ggtcaccgcc cgcttcgcgc
     5761 acaacgggag ctgattcacc cgtaacggca ctttcatcca ctgacgcaat tcccgcttcg
     5821 atctctccat ctgcaggcag gatatcgcca gtgacaacaa gatataagtc gttttttctc
     5881 agcaaagtgg atgaaatcac ctcgtgctcg ctggttgcac catcaagctg tgtttttttg
     5941 agttttttgg cgggagtatc ctgttttgct tttcgcagag actcagcctg tgccttgccc
     6001 cgcccttctg caactgcttc agaaaaattt gcaaataaaa cagtgaacca aagccagatg
     6061 gcgatcgcac cgataaaacc agatgacgct tctcccttcc caagcagggc ttgcacccat
     6121 aatacggttg tcaatgcgct gcccacttcc acaacgaaca taacggggtt gcgaatcatt
     6181 aaccgcggat tgagttttat aaatgcctct gttactgcac gctgataaat ttcgcggcgg
     6241 aaagaattgt gctttcgggt ttgaatgctc attttgtgtt ccttgaattt caatatcaac
     6301 caccgatgat tgtcagatat tcaacgatgg ggcccagcgc cagggcggga atgaagctca
     6361 atgcgccaac gatcgtgatg acgccgatca gccaggccat gaacagggga gtatgcgtgg
     6421 gcaatgtccc tgcgctggcg ggaattttct ttttgcggac aagtgaccct gcgatggcta
     6481 atactggaac agccagccaa taacgagaga tcaacatggc aatccccaat gaaaggttat
     6541 aaaaaggact gtttgcactc aaccctgcaa aagcagagcc gttattgttc cctgcagatg
     6601 aaaacgcgta taagacttcg ctgaatccat gcgccgttgg gttgtaaatg gcagccctcc
     6661 cgtcagggat tgaaacagcc agcgctgtac ccgtcaaaac cactgttact ggaatgagaa
     6721 tgatgaggga cgccattttc atttcatagg cctctatctt tttgccaagg tattcagggg
     6781 tgcgtcccac catcagacct gaaataaaga ccgctacaat gacgaaagcc agcatgccgt
     6841 ataagccaga accaacgcct ccgtaaataa tctcgcccaa ttgcatcagc cacatgggca
     6901 caagcccgcc gagcggtatg aaggagtcgt gcatcgcgtt gaccgatcca ttggaggcgg
     6961 ctgtggtggc agtggcccaa agcgcagagt taacgatccc aaagcggacc tccttgcctt
     7021 ccatgttgcc gcctgggttg ctggccgatt gaatctgatc gatgcctaaa gatgggagtg
     7081 cagggtttcc gccgagttca gaccaaatgg ttatgccgac gagaaccaga aatatgatcg
     7141 tcataactgt cagcaatgcc cagccttgac ggaggtcgcc caccatttgg ccgaaggtgt
     7201 agcacaaggc cgcagggatc agcaggatgg aaagcatttc cagaaaattg gaaaaagggg
     7261 ttgggttttc aaacggatgt gctgaattgg cgttgaaaaa tccgcctccg tttgtgccca
     7321 gttgtttgat cgcgatctgt gatgcggctg gtcctacagc aataacctgc tccgcagatt
     7381 gcaggggcga gacggttcta tatccgttga aggtctgcac tacaccttgt gagaccagca
     7441 acatggcaag aatgaacgaa agcggcagaa ggatatataa agtggagcgg gtgaggtcga
     7501 cccaaaaatt cccgatccct tttcctgagt ggcgggcaat tccgcggatt aacgccacca
     7561 aaacggccat acctgttgcc gcagagacaa aattctgcac cgtcaggcta agcatttggg
     7621 tgaagtgact catggtcgtc tccccgccgt agctctgcca attggtgttg gatgcgaaac
     7681 ttacggctgt attccaggcg gaatctggcg ttacagcaga gaatccctgc gggttgaagg
     7741 ggagtgcgag ttgcaatctc tgctgggcat aaacgaccaa taatcccaac atattgaaga
     7801 cgagcagagc cgcggcatat gatttccagt ccatgtcttc ggtggggcgg atgcctgagg
     7861 cgcgataaat taatctttca acaggaccca gtacacggtc cagaaagatg cgctcgtttt
     7921 gatagaccct tgccatgtat gagccaagcg gttttacgag cagtagtaaa gttcccatga
     7981 aaaacatgag ttgaagcagg ttgtttgtgt tcatccaaac cactccggtt tgatcagtgc
     8041 cagtaccaga tatccgaaca ggagcaatgt gataatgcca acgattgcgt atagtgtatt
     8101 catttcggtt cctccccgag cctttcgcag accgtgacaa ggccataggt gatggcaaag
     8161 aaaaggacgg taaaacccag gtataaaaga tcgctcatgg tttctcctcg catttgaata
     8221 cagcaagttt atgagcgact tggtcaattt ggcggcaaga aggcggggaa atgcatcaag
     8281 aaagcatcaa gaattgcaat gtggaaaccc gaaaatgggc tttggctagc cattccctac
     8341 ggtataattg cccccatgca aacgaatatt ataggtgtcc gatttacaaa aattggcaaa
     8401 atctaccact ttgattcttc cactcttcct gatgtgaagc agggcgaaca tgtgattgtc
     8461 gataccaccc gcggaaagca tctcggcgag gtggtgcaag ttttgacaga aacccctccc
     8521 cagcctgaag gtggttggaa ggctgtggaa cgccgcgcca cgccgcgcga tttactgctt
     8581 cagcaatcct ggcaatccag gcaaaccgaa gccatgatca actgccgtgc gcgtgcttct
     8641 gaattgcatc tgcgagatgt caaaatcgtc acggccgagt acaactatga cggttcccgc
     8701 ctcactttcc tttttagcac tgaaagtgaa gataaagtgg acctgaagtc cctcaaaaag
     8761 gacatgcaac aaatttatcc caatgcgcag atcgaaatgc gtcagatcgg tccgcgggat
     8821 gttgcgaagt ttttgggcgg catgggagct tgcggcatcg aaacgcgctg ctgttcgaag
     8881 ttccttactg atttttcgcc catttccatc aaaatggcaa aggaacaggg aatttccctt
     8941 acgcccaatg aaatcaccgg catgtgcggg cgtttgcgct gctgccttat ctatgagtac
     9001 gagcaatatg tggaagcccg caaaaccctg ccgaagcgca ataagcgcgt ggttacaccc
     9061 aagggtgaag gcaaagtgat tgatgttgtt cccatgagcg ataaagtagt ggtgctcatc
     9121 gaaagcggcg ttgaccgccc ccaacaaatg acctttgctc gtgaagagat cgaaccgtgg
     9181 gatgaacttg aagccttgcg cagaaaatcg caggccccct gtgaccgcca cgaaggcggc
     9241 ggctgtacct gcggaaaagc tcctcgcaac aacaataaca aaaactaatt taaaaagcac
     9301 gaattgcccc aaaaagattc gtgctttatt ttcaaatttc ccatgaccct tcaacctgaa
     9361 aactggactt ccccccaaaa tattcttgtg attcttgccc accccgatga ccccgaattt
     9421 ttttgcgggg ccaccctggc ccgatgggcc cgcgatgggc acaagatcac ctaccttctt
     9481 ctgacttgtg gtgataaggg gtttaatccc accacaaacc cagaaatgac gcccgaaaaa
     9541 ttatgcggat tgaggcatgg ggaacagaat aacgcagcaa agatcatcgg cgcaaactcg
     9601 gttcatttta tggacctccc ggacgggtac ctcatcccag acctgaatct acgccgtgac
     9661 attgtccgcg agattcgccg ccacaagccc gatatcctcg tcacctgcga cccgcagact
     9721 ttgtttgcca cctacggtat taatcatccc gaccaccgcg cttgtggtca ggtggttttg
     9781 gatgcagtct tccctgctgc gggtaatctt gtttacttcc cagacctgct tgcgcaaggc
     9841 ttcgagccgc atatgccgaa agaggtctgg tgttcactta ccaatcaacc caacaccagt
     9901 gtagatgtga ccgatacctg ggatatcaaa atgcaggcca ttcttgaaca caagacacaa
     9961 gttcaggatc ccaaggcttt gatcgaacgt ttcaaaaccc gccgcactga agacagcacg
    10021 gaggaaaatc cgcgctatga agagaaattc aaagttgtaa agtacggata atcaaggatc
    10081 aaagggtcta acaacccttt gatcttccaa aaatgaacat tgtcaacgtt ggttaccaat
    10141 cgacaaatta ttatctcatc gacattttag gcgggaaatt gctcgttgac tgtggttggc
    10201 cggggacact tccacaattt tcagcggaat tgaagcgaaa agggatcaga cttcaggaga
    10261 tcaaatatct tcttgttacg catttccacc ccgaccatgc tggcatgacc caggaattaa
    10321 aagcgattgg aatcaaacac attctattgg atgtgcaggt ggatttcgtt tttcagatga
    10381 cggaattttt taagaccaaa ccccatccct accggccaat tacgacggat gacagtatca
    10441 ggttgaaggt cgaagagagc cgtgaatttc ttttgaagct ggggctttcc ggggagatca
    10501 tccacactcc ggggcatagt gatgatagtg tgtcactttt ccttgatgaa ggtttcgtat
    10561 ttacgggtga tttgtccccc atttttgtat taccagagag tgataccatt tcacgggcaa
    10621 gctgggatag aatttatcaa caccatatca aaactgtatt tccaggccat ggaaaataat
    10681 caggagaaat caatgaccga ttttctgaat gaagccagat cgttattttc atacactcaa
    10741 accttgcgcc gtgattttca ccttcaccct gaattgggtt tcagggaaat tcgaaccagt
    10801 ggaatcgtgg caaaggaatt ggaagcgctt ggccttgaag tgacaaaagg tgtaggcaag
    10861 acgggtgtgg ttggcttgct tgaaggtgca aagcctgggc ccaccctgct cctgcgtttt
    10921 gatatggatg ccctgccaat tcacgaagaa acaggtgcag aatatgcctc gcaaaatgcg
    10981 ggtgtgatgc atgcctgcgg acatgacggt catactgcca ttgggttgac tgtggcaaaa
    11041 ctgctgaatc agcacaaaga caagctggct ggtacgatca agttttgttt tcagccctcg
    11101 gaagaaggga ataacggcga agaagttggc ggcgcagaaa tgatgatgcg tgatggcgtg
    11161 ttggatggcc ccaaggtgga tatgactctc tcattgcatc tgtggaatga aaagccgctc
    11221 ggctgggtaa atgtggccaa aggcccggtg atggcaggtg ctgaggaatt taagatcaag
    11281 gtcattggca aaggcggtca tggagcgatt cccgatctgg cagtagaccc agtggtatgt
    11341 gcagcgcaga tcgtttcggc ttcgcagtcc attgtttcgc gcaatgttgg tccgttggag
    11401 acggcggtca taagctttac ggtcatcaac ggaggcactg ccttcaatgt cattccgcag
    11461 gaggtgatga tgcaaggcac gatccgtacc tttgacccac aagtgcggat gaaggtgcta
    11521 cagcgtttcg atgacgttgt gcgcggggtt gcctctgcaa tggggtgtca ggtggagttg
    11581 aatgtgaagc agcttacccc tgcgctgatc aatgccgaaa gagtggcggc cagcgtgcag
    11641 gccactgcac aacgtctgct tccaaacctg catcatgacc tttcacctta cctgaccatg
    11701 ggcgccgaag acatggctta catgcaggcg aaagtggatg ggtgttattt ctttatcggt
    11761 tcaaacaatc atgagcggca tcttgattac ggtcaccatc accccaaatt tgatttcgac
    11821 gaacaggcgt tgatcaccgg ctcggcgctt atggcggcag cggcagccga cattttgaaa
    11881 tagtatataa tgaaattcag gtagaaattg gagaaaggat atcttatgaa aaaactaacc
    11941 attaccattt tgcttttgat gctacttctg agcgcatgta cgcctcccgt atcgccgacg
    12001 gaagcgcccc ttgcaacaga agaaccatcc caggttgggc ctgcggtgga tatacccgcc
    12061 aaccccgaac ccctgtccac tgcaaccgag gctcctgtag cgcgtgaggc aaagatttcc
    12121 gaagttcaaa atgaagtttt ggcacggacg ctttccacca gtgatctggc gcccgcttct
    12181 gtgggaacaa gtattcttcc aaccggtggt gtggaaacag ggacggatgg ccgcgcccgc
    12241 ctcgacctgt tgcctgaagg cacgatcgtg cgcgtgggtc caaactcttc ctttaccgtc
    12301 ccggagatcg tccaagagaa tggccagcca aagaccgtaa ttgaattgct gtttggaaag
    12361 gtttatattc ttttgaacgg gggctccctt gaagtggaaa ctccctctgg cgttgcttcg
    12421 gtgcgcggca gtttgatgcg tgtggaatac gatgccgaga cgaaaagaat caaggcctct
    12481 tgtcttgagg gacattgtgc ccttaagaat gaaaacggcg acgaagttga gctggttgct
    12541 ggtgagtaca cctatatcga aggcaatttt ccgccgattg atcccttgtt gatcgatagc
    12601 tcggagatcg aagaatggct ggctgaaatt cccgaactgg gagattttct aaatccactt
    12661 cctgacccga aggactaccc tgacgttgaa gatggaaacg acaatggcaa cggcagcaac
    12721 gacaacggta atggaaacga taacaacaat aacgacgatg atgatgacga tgacgacgat
    12781 aacaacaacg gcggttatcc aaattaatca tcgactggga tcataccgac taaagacact
    12841 gatccttgcg tttcctgctt gaccttttgg tgggtgcgtg cttcgtggca gattcgccgc
    12901 cggggctgca cccaccaatt cttttgctga tcaccatcca tatcagatgg cgaggaattt
    12961 ttctcaatga ctaagtttca aaaatggatc accaccgccc tggtcgtctc tgtggcggct
    13021 ggggttttta ccctgcaaaa taaccttcaa ccgccatcgt ctgaaaatgt tcaaagtaca
    13081 gacagtaccc ttacccctga gatcgcagcg cctgtcgaat ccctcacccc aacactggca
    13141 tatgaaggct gtggtttcat gtgggccact catgatgcgc ccgaactgac agagaagatc
    13201 gatcttgcgg tccgcaagat gaatgacaaa gcatgggcca atgcaaactt tttcggggag
    13261 gattgtgttt atgcagatgg gcgtacaact ttcggcgtga tggaaacgga tgtttttatc
    13321 cacctgcctg tggatgattt gttagataac gagtttttcg gagattggat tgctcaggtc
    13381 atggccatga ttgtacagat tccgaaagag gaatttcaaa acaggtatgg atttgtggaa
    13441 ttctggtttg aagcgagtga gacggatcat ttggttgtgc gtgtgccgat tcaaaaatac
    13501 ctggaagtgg ggcagggtgt cagtggggtg gagatgttgc agctgttcat tgagacccct
    13561 tgatcgaaat gtgaattttt gtaagtgttg aaaagccgtc ctttggacgg ctttttgttc
    13621 ttgacaaggt cgggtattta tcgtattata cgtttagtaa ttactaaacg taataaaagg
    13681 tagatatgcc cataaaactt tcccaactta aacaggattt tacagaaggt ctaagccaga
    13741 tcagccgttt ctgggggttt cccaaaggaa tgggagcgat ctttgccgtg ttatatcttt
    13801 cccaaaatcc gctttcgctg gatgagattg tagaggcgac aggcttgacc aaaggagcgg
    13861 tcagcaccga ggtccgcact ttggcgcgga tggggttggt gcatcgttct tccaagctgg
    13921 cagatcgcaa agattattac atggccgaag atgatttcta caagtcgatc cgttcaatct
    13981 tgaaagaacg tcagaataac gaattcgata gcgccattcg ttcagtgggg gatgcccttt
    14041 ccaaacttga ggcgggcgat gtgaccggtg acaaggcaga ggttgacttt acctacaaac
    14101 gcattcaagc cttgcaggaa ttcttcaatg ccattgacag cctcacgcgg gcggttgtga
    14161 aattggaaag ccttgggttt accaacgtta aaactatttt gtcggttcta aaataaggag
    14221 tcgaaatgaa aattgcagtt acaggtgctt ttagttactc aggcaagtat attgccggtc
    14281 gtttgcttga acgcggggaa gaggtgttta ccctgacaaa tcaccccgat cgtcctgatc
    14341 cgtttgatgg gaaggtaaag acgtttccat tggatttcaa tgaccaggaa ggattgatcc
    14401 gcaacctgag cggtatggat gtgctggtca atacctactg gatccgtttt gacaaaggtg
    14461 ataacaccca gccgcgtgcc gttgaaaata cgaaaatcct tgtggaagcc gccgcaaaag
    14521 cgggtgtcaa gcggattgtg catattagca tcaccaatcc gtctgcaaaa tctcatctgc
    14581 cttacttttg gggcaaggct gccaatgaaa aggcggtcat ggagtctggg atgagttacg
    14641 caattctgag gccgaccgtc ttgtttggta aggaagacat tcttatcaac aatattgcct
    14701 ggctgcttcg aaggctgcct gtttttggcc tgccgggtga tggctcatac aaactctctc
    14761 ccgtttacgt agatgacctg gctgagatgg ccgttgatgc agtttacaag aaagagaatt
    14821 acatctggga tgcagttggt cctgacgaat ttacattcaa agagatggtg gaattgatcg
    14881 ggaagtcgat tggcgtcaaa aaattattgc ttccttttcc gccgcggctt gcactactgg
    14941 ctgcccaatt tatgagcctg tttgtcaaag atgtgatgtt aacccccgag gaagtggatg
    15001 gcttgatggc aaacctgctg gtttccaaag aggcgccacg ctgccgtacc caccttagtg
    15061 attggctgga gaggcatcgc gcttcggttg gaagtcagta cgcttctgaa ctggcaaggc
    15121 atttctaaaa aaacaaaatc cgctctaagc ggattttgtt ttagtctatt ggcaccaagc
    15181 ctgcgcaacg tggactatcc atacagccat agtatttttt ccctgaacgc ggaccatcgc
    15241 ggttgatgcg taaaaccatc atctttccgc ataccggaca aaggggtact gaatcagaag
    15301 aggtatcaag acgtaaagca gcagtcccgc caactttggt gacagcgagt tgaaacagag
    15361 agacaatatc ggagggatcg taggtctcac tggaggggac atgaaccagc ggcagacctg
    15421 catctatgaa taactcttca atgaacctgt cgctttcgcg ggtttcattt tttgcgatcg
    15481 gccttacgat ctcaacacca aaggcagggg caagtgtttg cggatcacag agtagaaaat
    15541 caacatgttt tcggaaaaac ttgttgaaga agtgtacatt ttcatttggg cggacgatat
    15601 aaaaaatatc gttgagtgcc actttaggac agaccagata tctcgcccca accatctgtt
    15661 gcaacgcacg aaaaagtgcc aattctgtgg ttgcgaggaa aggctcgcgt aacctgtaag
    15721 gcaatctctc ggtgttggac atgggcggat tataaattag gatgatgttt gctagcaagc
    15781 aaatcagcgt tttaagagcc ttgcatcagc gtgatcactt cctgtttcag ggcggcgttg
    15841 catgctaatg catcacctgg ggtatgacct tcctccccag accaggtacc aaaatacccg
    15901 ccagcctctc gcaagattac aggatatggt ccgtaatccc aaatatccag aaatggatca
    15961 atacatacct cggcccgccc ggtggcgacg agcatgtacc catacgcatc accccaggtt
    16021 cgaagcaggg ctttttttct ggcgaagcgt tctctcaggg tggggtcttt ttcttcaaga
    16081 cgttcaaaat ctgaagtgac taaacaggcc ttggaaattt catccacgct tgaaacatgg
    16141 gcgcgacggc tgttccacca gcagcccagc ccgtcagcgg cacagagcat ttcatccaat
    16201 ggcggataat acgctgcgcc gacactgatt cggccttcta tttccagccc gatcagcaca
    16261 ccgtataacg gcacgctgcg gataaatgac ttcgtcccat caatgggatc tatggtccag
    16321 cgaaaagggg agccgtttcc ctggctatgc ccgaattctt ctccaatgat ggcatgaccg
    16381 gggtaatttt tttcaatttc agcgcggatg aatttttccg cttcgtggtc ggcaatggtg
    16441 accggctcat tgttttcctt catatccgca cgcactccgg tgttgtaata acccattgtg
    16501 attcggcctg cgcggtatgc aagggaggtg gcaaagtcaa gatatggttg gaggttcata
    16561 acaatgtatt cagcaactcc ttttcctctt tttctgttgt gatctttcca tccagccagg
    16621 cggcgcggag ctgggtgaga atttcgccgt aacgcggacc gggcggaagg cccagcgctt
    16681 tcaggtcatt gccggtggtg cgggctttaa catgacgcca gaatgagatg taactcaaca
    16741 acgccccttc tcctgtttcg agatacaccg cgtacactgc caacagcggc agattttcaa
    16801 gggccgatgc ccaaacactt ggtgacgagc cgatcaggaa gggcagactt tttcttaata
    16861 tcgaagccgc ccataccgaa tgagttaatt ccgatgtaaa ttccaaccgc cgcgaaatgg
    16921 acacgatgtc atcttctgaa agatcaagca gccaaaacat atagcccagg gtggtgcggc
    16981 tggcgggcac ttccaaacct gtttccattt caaggtgatc tgcgtaagtg gggttgaatg
    17041 ctggcagctt cgggtgaatg tatcttaaag cgtcaaattc acttaatctc tggagcatgg
    17101 tcagcgcttt ttcttcttcg aagatcaggt caagttcgtg tcgaagcctt tcgccactta
    17161 acatcctgaa aacatcaagc gcttcagcgt tgaccaattc gcgggtggct gggtcaagtt
    17221 gaaatgaata gcggctttcg tatcgaatgg cgcgaaagat tcttgttggg tcatcgataa
    17281 atgaacgggg atgtaaaacg cggatgattc cctgcttcag gtcttgctgc ccattgagtg
    17341 ggtcgagcaa ttcgccgaag tgttcgccat ccagacgaat tgccatggca ttgatggtga
    17401 aatcgcggcg gcgaaggtca tcttccatgt tcgagggggt tacgtttgga agcaaaccgg
    17461 gccgtgcgta ggtttcagag cgggctgtga tcaggtcgag aaagggagtt tgaatttgtt
    17521 ccgcaagaga ttctggtaca tgccagatgg cggtatgaaa tttttgatgt gcggtcagcc
    17581 tgccgccgaa gcgattgaca aggatattcc caaaactgat cgcatcccct tcgaagacaa
    17641 tgtcgaggtc gtggatgggt tgttcgagca gcagatcacg caccgagccg cctacaaggt
    17701 ggcagggaat cattaactct gctgaaaggg aggaaatgtc tcgcagcaga gtcagttgtc
    17761 ggttggagag gatgcgttcg ttttccaggt ccagtttcat ttggcaggcg gcctttgatc
    17821 catcactggg ttaagttcct ggcgtgttcg taggcttttt ctgcgtattc cattttccct
    17881 tcctgggtta gaacatctcc aagcaaagtc caaatgacag cttcgttggc atgctcgcgc
    17941 acgtattcga tcatgtcctg gcggatctcg ttcaaatttc tgcgatgctt tatcaaatga
    18001 gaataggcat gtatggcatg ttcaaaatct ttttttgcga tggcttcacg tgccacttgt
    18061 aaaacgggat gcttctcttg tgaatggctc acgggtggat tgttttgtaa ggtcacagaa
    18121 attccttgcg tcgctgtttg tgctgcgaca gaatcagagt catgatgaat agattttgaa
    18181 tcacttgccg attgcgcttc tttcttacct ttggcctgct tccgtttaat catagacgtg
    18241 ccaccgtagg catttgctgc ggggtcaagg acttccaaca acccatcgcc cagcagattc
    18301 caaccaacac caaaaaggac aatggcaatt gtaaccggca ggaagaccca ccaatagcgg
    18361 agcaagcccc cgcccgggcc aatgatccaa ttgcggccaa tggagaggag aatgccccag
    18421 gctgaagcgc cgcctagccc tacaaaagtg atcgtggctt gaagaatgac ggaacttccc
    18481 acatcacgtg ctgccaatac cacggcagga ccaaccgaat tgggaatgag atgacggaag
    18541 atgatccatg cagggctggc gccaagtgcg cgggaagatt gaatgaattc cttgttcttc
    18601 agggtcagaa ccattgtgtt gacgatgcgg gcaacaggca tccatgaaaa aatgatcaag
    18661 ctgatgagga tggggtcgac cttcatcaga aaaacggcga agggcggcgg tgtaaattga
    18721 aaatcgatca cccgaccaaa gtagtcattg ttgagccagt agattccccc cagcgattcg
    18781 atcgtgactg ccacaagttg ttgcagcagc accacccccg ccaccactgg aaaggtgagg
    18841 aacgcatctg cgatcctcat catgatgttg tttacaaatc cgccgaagta acccgctaca
    18901 gaaccaaaca gggtgccgaa aatgaaggcg ccgatcgcca cggacaggcc aaattgcatg
    18961 gcttcacgcg ccccccaaac cagggtgtga tacacgtcaa tttgccccgg cagggttccg
    19021 aggatggagg tttcgcttgg cgggtggggg gttctatcgg aggaacgacc gacctttttg
    19081 aacgaacctt gggtgttttt atcaggcggg gagagcaatg gtgcggatat ggccaccaca
    19141 gtaaacatca gtacgatcag cagaccgagc caattttgcc agcgtgaaaa gaaatagcgg
    19201 acgattctca tgctttcaaa acttcctcgc ggacacgcgg gtcaagggtg gcttgaataa
    19261 agtcgaggat gagcattagc ccaagcacca tgatgacact gtacactgaa aaacccaatg
    19321 cggcgggagc atccggatag ctgctcattg cgatcacaat gatttgcgat atccccgtca
    19381 acccgtaaat gatttcggtg acgaacagcc cggtcacaat gctggcagcg gatagggcca
    19441 tggctgtcaa cgaaggcgcc agaatggctc gcagggcatg tttccagatc agcttggatt
    19501 ctttcacacc acgggcacgg gctgccacaa tatattcctt gccgcgttcg ttcatgatgc
    19561 tggcacgggt gatacgtcca agggtggccc aatggtaaat tgacagggtg aagacgggca
    19621 tgatcaaatg ttttagcgcg atgatgaaaa tatcgaagcg tttattgagc aggctgtcga
    19681 gggtcagcgc cccggtgtat tccacaaaac ccgaacggaa catttgcagc tcggttttta
    19741 catcaatgcg gcctggggca agccagtcta gttgaatgta aaggatggag atcagaatga
    19801 tcgaaacaat aaaagatggc gttgaggtgc caaggaaggc gagtccgcgg aagccgctgt
    19861 caaacatgct gcctggccgc caccctgaga ccagtccgct ggcgagcccg agcggaatca
    19921 gcatgaggag ggagtacagt gccagttcca atgtagcggg agtacggtgc aaaagggatg
    19981 gcaatacatc tgcattgagg gtcgggctgt agccccatga cccttgcagc agggattttg
    20041 cccaatagaa atattgaacc agatagggct ctcgaaggtg gttctctttg atgatgtttt
    20101 cgatgtattg ttccgaagcg cgttccccgc ctttgcctgg gggtaaatag atgcgtgccc
    20161 gtgcttcagg tggagtgagc atgaagcctg aatacaacac catggtgatg atgatcaggg
    20221 aaataaatgc agaaaatata cggcgtatga gaaattgcag aatcgggggc atggggttat
    20281 ctgatagcct gaatataaca gaatgctgga ttgaatgaaa tacctgctca atgcctcaaa
    20341 agcgcacagg atttgtcttg gagtgatttt cacctgtatc ataacaaatt ctgccaaaat
    20401 gtaaggaagt ttcttctcat ttataaatat gtgtgtataa ttggcgcatg tttaagccga
    20461 aaccatcccc tcaaagcccc ttttttaatt cctccaggtc tggttaggtt cggttcgatg
    20521 cgcccaaacg gctcccagac ttggtgagcc gtttttattt gcaaattgga aaaaannnnn
    20581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntggca tccgtttcag gtattgacct
    20701 tgaagctgcc gtgctaaaga aaattgaagt gaacaagaca agaaaatggg atgttgagga
    20761 atagttgaaa gcaacttccg aaaccttcaa ccttcaacat taaacattca acccatccac
    20821 aaggaatctc atgaacatca ctgtatttgg cggagcacaa ccaaaagaag gttcacaggc
    20881 atatgaagaa gcccgtgaac ttggctcgct tcttgctcag cgtggacatg ccgttttaac
    20941 tggcggttac atgggcacaa tggaagctgt ctctcgcggc gcgcatgaag cgggcggaca
    21001 tgtcatcggt gtgacgtgta tcgatattga agaatggcgc aattcaaagc caaatcaatg
    21061 ggtaaaagaa gaacggcgca gacaaaccct gatggagcgc ctgatgggat tgatcgaagg
    21121 atgcgatgcg gcaatggccc tctcgggcgg tgcaggcaca ctgacggaga tctcgctgat
    21181 gtggaatttg atgatcgtgg aatcgctgcc tcccaggcct ctaatactgg tcgggagcgg
    21241 ttggcagtcc acttttgacc agttcttccg tgaatttgaa agttatatgc ccaaacatca
    21301 gcgcgaattg ttatacttcg caaaagatgt caaaactgcc gtaacaatat tacaaagctg
    21361 atggatctgc caaaatcatt ttttatttgc gatctgtcaa cttgcaacct gaaaccgata
    21421 agaggaaaaa atggctgaag aaaagttaac aacccgtgca gaagattttt cagaatggta
    21481 caaccagctc gtgattcgag cagacatggc cgactacgcc cctgtgcgtg gctgcatggt
    21541 ggtaaagcct tatggttggg cgctctggga gaatattaca tcctggcttg atgcggaatt
    21601 caagaagacg ggacatgtca acgcggcttt cccgaccctg atccccatgt ctttctttga
    21661 gaaggaaaaa gatcatgtgg aagggtttgc ccctgaattg gcggttgtca cccatggcgg
    21721 cggtgaggag ttggaagaga aactggccgt tcgccccacc tctgaaacca tcattggcca
    21781 catgtattca aagtgggtca aatcatggcg cgatttgccg attctcattg tgcaatgggg
    21841 gtcggtgttg cgctgggaac tccgcaccaa gctctttctg cgcaccgctg agttttattg
    21901 gcacgaaggc cacactgcgc attcttccga ggccgaagcc aaggaagaga tgtatcgcat
    21961 cctcgatatt tatgagcgtt tctgcctcga agaagcagcc atccctgttt tcaaaggcac
    22021 aaaaagcgat accgagcgtt ttgccggcgc acagatcacc acctccattg aagcaatgat
    22081 gtgggataag cgtgccctgc aatcgggcac ctcccattat tttggcgaca atttcgccaa
    22141 ggccttcgag atccaatatc tcgataaaaa caacaaattg acctacgccc agaccacctc
    22201 atgggccatt tcttcccgca tcattggtgc aatgatcatg gtccacggcg atgacaacgg
    22261 attggtcctg cccccgcgcc ttgctccgat tcaagcggtg gtcgttccga tcttcaaaag
    22321 cgatgaagag aaggccaaag tgctggaagt cgctgaccga atttttgccg agctaaaggc
    22381 cgccggtgtc cgtgtcaaat tcgatgaccg cgacaatgtc agcagtggat tcaaattcaa
    22441 tgattgggaa atgcgcggtg ttcccgttcg tgttgagatc ggccccaagg atgttgaaaa
    22501 ggggaccgtt gccctcgccc gccgcgatcg ccccgggaaa gaaggcaaag ccttcgtctc
    22561 acaagcgaat ctggactcaa ccgttaaagg gttgctggac gagattcagg cttcgctcct
    22621 caagcgcgcc accgaatacc gcgacgcaaa catccacaat gtgaccacct tcgacgaact
    22681 caaggaagtt gtgaaagatg gctgggcctt tgcctattgg tgtgaatcac gtgagtgtga
    22741 agccaaggcc aaggaagaaa ccaaagccac aacccgcaac atccccttcg accagcccaa
    22801 tgaggaaggt gcctgcgtcg tctgcggcaa accctccaag cgaaaagtct atttcgcgaa
    22861 agcatattag tgagtgtcac gtgttcaagt gtcaggcatc aggttacaag ccgattcgcc
    22921 tgacacttgg cacttcgcac ctgacacctt cttcccatgc ccgccattga cctcgcccgt
    22981 cttcgtaagc aagccaaccg tctggcagat ttcttctttc tgccagatga atttatgaag
    23041 catctgcgcg agatccttga tttttacgtc aattacaccc tgcggacaaa agaaaacgtt
    23101 gcccctggct ccaacctcaa aacctatcgc acaccgcccg cagtgcttac ccagatcgaa
    23161 aatgaactgc gagcggtggc aacagccaac cccgaccacg cactcaatct tgccgacacg
    23221 ctttgggatg aaggcgcact cgaaacccgt ctgttggctg cttttttatt aggccgaatc
    23281 cctccgcaag aagaacgcct tttgccccgc ctcaccgcat ggactcaaca aatccgtgat
    23341 ccagatgttc gttcagcgtt gctttccaca agcctggcgc gcatgcggcg tgaaaacccc
    23401 aatcaatttt tcaacctgat ccgcgaatat ttgcatcctg cccgtacccg tacgtggtca
    23461 aatggtattc aagccctgat tcccatgatc gcggatacca ccactgcaaa tcttccaccg
    23521 atcttcgacc ttgttgagcc gatcattgaa gaagccccat ccacgttaca gaacgacctc
    23581 gcagagttga tcgtggttct gtaccgtgcc tcttcgaatg aaacgacctt catgctcaag
    23641 catatccttg cgaatactga aaacccgatg accgtggtga ccctgcgccg catttcatcg
    23701 gcatttccgc cgccgcttca atctgcatta cgtgaccttc tgcgcccgca gccattatca
    23761 aagcccagtc ccgttgaaga tgcggaatct tcagattttg tcacagaaga aacctccgaa
    23821 cccatggtaa agaaaccgcg cagaaaactt accacacgcg aacccaaacc cgaatccgct
    23881 gccaccgata aaaagaccgt cacccgcagg aagaaaaaag acgaaagcgc atccaatgga
    23941 taactcaaag ataatctacc ttcacggcct tgaaagcacc agtcagagcg gcaaagcccg
    24001 ccagttcgcg cagttatttc caggcatgat cacccccgat ttcacaggct cctttgaaga
    24061 acgcatggcc caactggctc cgatccttgg cgataaaaaa gattggacca tcatcggttc
    24121 ctcttttggc ggattaatgg gaacggtctt cacctgcgag catccatctc aggtacggaa
    24181 attaatcctc cttgcgccag cccttctccg cgaccatttc gcttcttatc tggacctcga
    24241 acctgtttct gttccaactg tcattatcca cgggaaagaa gatgacattg tgccgctgga
    24301 acccgtgcga atactggcag agaaaacctt ttccaacttg caatacattc tcgtggaaga
    24361 tggtcaccgc ctgcacaaag ccttcgatga attgaattgg aaagannnnn nnnnnnnnnn
    24421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24481 nnnnnnnnnn nnnnnnnnnn nnnnnaggtg cataatgaaa caacacattt actggcacac
    24541 caccgtccaa atgcctgatg cttccaatct aacacccatc cccgaaaaag cagatgtggc
    24601 gatcatcggt ggtggatata caggcttgag cgccgcgcgc acttttgcaa agaacaatgt
    24661 ccatgctgtg gttttggagg ctgaaaccat tggctggggt gcaagttccc gcaatggagg
    24721 catgaccctc actggcctca aggtggccat gcaaaccgcc atcacgcgtt atggacgaga
    24781 cctggcgaga agtttgtttc aagcttcgat tgattcagtt tcacttgtag agcagatcgt
    24841 tcaagaagaa aacatcaact gtgggtttgc ccgcacaggc catttgttgg cggcgaataa
    24901 gcccaagcat tacgaagccc tcaaaggcga agtggatttt atggcaaaag aattcaacca
    24961 taaagttcat ctggtttcgc cgaaggactt gcataccgag atcggtacag atgtgtacca
    25021 cggtgccttg gtggatgagg tcagcgcggg gttgaatcct gctcaatatg tggcggggct
    25081 tgcagcggcg gctgccaaag cgggggcgat tctctgcgcg cgagcgcgtg tcaacaggat
    25141 tcggcggaac gggacccgtt ttatcgtcga aacagaaaga ggctcgcttg aggttgattc
    25201 tgttttggtg gcaacttctg gttatacagg caatgtcacc agaaatttgc aacgcaagat
    25261 cattcccatc ggttcattca tcattgccac tgaacggctt tcgaatgaac tggcaaatga
    25321 gcttagcccg aagaatcgta tgatctttga tttcaagcat ttcctgaatt attttcgttt
    25381 gtgggataac cgcatgatct tcggcggacg ggcggcgttt tttcctgaaa atgaaaatac
    25441 agtggcccaa agcggcgaga tcctgcgaaa tgaaatgcta aaagtgtatc cgcagttgaa
    25501 agatgcgaaa gtggaatacg tttggggcgg cacgcttgat tttgcatttg accaaatgac
    25561 ccatgtgggc gaagaagatg gaatgcacta ctcgcttggg tatgcagggc atggtgtggc
    25621 aatggcgacc tatctcggta aaacaacggc tgaggccatg ctgaagggca atatcaaaga
    25681 gcatcctttt gcgaagtttg cctttcccgc ggcacctctg gggctttaca caggcagccc
    25741 atggtttctt ccttttgcag ggatgtggta caagattttg gattgggtgg aataacgacg
    25801 cgatttcagt gggctgtacc cttgtaacag atctcttcgg gcaggtttag tgcttgagtg
    25861 ctgaccgcta caatgcgcac ctcagggcat gcaggcagga ttgtttttat taactgcacg
    25921 catgcaccgg taagatgccc gcaatgagat aaaagcaaca acagatgttt ctcgcgtaga
    25981 aactcgatca aggcgatcac aggcgggcga ttgggtattt cacttacgtt gagaacttcc
    26041 atgacggttg gcattaccct ggcggggtca tggatcgacg ttaattgaat cagccagata
    26101 ccatcagaaa atatgtcttt tgtttgtgaa gcggccgcaa gtgttggagt ctcgtcgttg
    26161 ctcagcaagg taactaaacg gctttttgtg agcaagtcga ttagctctgt aacgggttct
    26221 tgtggtgcgg cggggatggc atctggcaaa tttccaatgg tggcggctgt ttttgcgatt
    26281 gagattcgct ctagcgcaaa acccacttcc tgagctgagc tgaaacgttc ttgcggcgtt
    26341 tttgccaaca ggcgcatcac aatggattcg agtgcagacg gcacatcagc ccgcgattgg
    26401 ctgggcaggg cgacttgttc attgagatgt tgcgtaagaa tggtctgttc atcaacattc
    26461 gagaaaggga cacgacctgt gatcatttca tataaaactg cgccaaacgc atacaggtcg
    26521 gagccaacgt cggcgggctc gccattgacc aattcggggg caaggtaaaa gaccgttcca
    26581 gccaggctgc tttcatttgg aagataggcc tcaccgcgtg ggcgcgcgag accaaaatcc
    26641 agcaacttga cgaaggtttg aaatccgcgt ggttcgagaa tgatattgct gggtttgagg
    26701 tcgcgataca cgaatccctg atcgtggata tatgccagcg catcacaaac ctgtctggcg
    26761 atatcaatga ttctggcgta ggtaaggttc ccagccttat cgaggcttgt gccttgaacc
    26821 agttccatga cgaggaaggg gactgacttg ccgtctgtgt ccactgtccc tgcttcatgg
    26881 gttgtcacta tgtgagggtg ttgtagcttt gttgaaattt ccatctcgcg cttaaattga
    26941 tcaagtgaca gggcattggt agtggcggcg ttgatgattt ttactgctac ttccctgtca
    27001 ttcaaaatgt cgtgcgcgcg atagatgatg cccattccgc cgcgcccaat ttctgcatcc
    27061 agacggtagc gttggtggat ctttgtccca acgccaaatg atgtttgagt gtgggcttca
    27121 tctttttgcg gcgcttttga taagcggcca aacagttttt tcatgactta attatattcc
    27181 gctcgctggt tcgggaaaag cggaaacttt cccatgattc aaatgcgcta taatattttc
    27241 gggaattcac aatgcgtatt ttggtgatat cggatattca tgccaacttt actgctttcg
    27301 aagctgttct aaaagatgct ggcagtttcg atgcggtgtg gtgtctcggc gatgtggtgg
    27361 ggtatggccc tgaaccaaat gagtgcatca acagactgcg cgaattcaac cctgtttgcc
    27421 ttgcggggaa tcatgacatg gccgtggcgg gcagggctgc agtgtgggat ttttcgaaag
    27481 acgcacaaga gatcattttt tggacgcgtc acaggctttc cacccaaaac cttgactggt
    27541 taaatacatt gaccgatcga ccgcttatga cagatttcgg cataaccctt gtccatggca
    27601 gtccgcgtga ccctgtttgg gaatatatga ccgacacggg tattgccaaa agcagctttg
    27661 aactcatcga aacacccatt ggattgaacg ggcatacgca tttccctgtc atttttcgga
    27721 agccctggga tgactggcgg gtgctggagg aatatccgcg catcaattct cccttaaaac
    27781 tgactctcga ccgaatgtgg atcaaccccg gaagtgtggg gcagccgcgt gatgaagatc
    27841 cccgcgcagc ctatgccatg atcgacactg aagaaatgac ctatgtatac cgacgggtgt
    27901 tgtacgatgt ttccgcggtt cagaagctga tgaaacagga gaaattttcc aatcgcctga
    27961 taaggcggtt gcgttttgga caatagcaaa aggagatgaa accatgtata ggaaaatttt
    28021 ggttcccctc gatgggtcaa agattgcaga aggtgttttg ccgcatgcca agtcgttggc
    28081 gtattctgaa ggggccgagt tgattttgct aacagtcggg gcaaaccctg cgcttgattt
    28141 tgctttttca gatcctgggc ttgcccaaac tgcagtggat gagcaagagg aaaaaagcaa
    28201 ggcatatatt tcccggatcg aatctgagtt gaaggcggac gggttcaaaa cctccacgct
    28261 tttgcgggtg gggtcggtgg ctgaggtgat tcttggggtg gcagaggaat tacaggtgga
    28321 tgtcattgca atgtccacac acgggcggac tggccctgca cgttggctgc ttggcagcat
    28381 tgccgaacgg gtggtgcata attcgaatgt gcctgtgttg ttgatccgtg ccagagggta
    28441 ggtttgaggg gcgatcattt ttgcgcgtga gcgcgagtca gcaggctgtg gttaaggttg
    28501 aagcggggtt ttcatctgct gcgctgtaaa gtctatgtga caaatgtcgt aaaaaataat
    28561 gacatttgtc tttgtttttg ggtgtatcca gttcggtttt tttgtgtttt ttcctgaaac
    28621 attaacgaat ttttattggt aggcggatgg tgattctcat cttgtgcaag gtaggtgcgt
    28681 aatggaatcc tctccccagc atgtgattca gtttgagctt cttttttcaa tgatttgggg
    28741 cgaggaaaag ctttccattg gaaaaggagc gccgatgact gataccgcct aaatcttccc
    28801 ctcttttgta aaactgcgct tttggcgcgg catatattca actgtcattt ccaaaggaga
    28861 atccagctta tgtctcaagt gactgcaaac acacctgcca gtggtgagca ggtggaacaa
    28921 ggtttacccc gcattatttt tgcgtctgct gctggtacca tgatcgaatg gtacgatttt
    28981 tatatttttg gcagtctggc gaccgttatt tcaagcaagt tctatcagac aggcactccg
    29041 ttaggtgatt tgattgcctg gctggccgct tttgcagttg gctttattgt acgtccgttt
    29101 ggtgcgattg tatttgggcg tgtgggtgac ttgatcggac gcaagtacac cttccttgtg
    29161 accatgaccc tgatgggtct ttgtacgttt gcagtgggtt tgttgcccac agctgaaacc
    29221 attggacctg cggcaggtat tattttgatt gcgttgcgca ttttgcaggg tctggcgctc
    29281 ggtggtgagt acggcggtgc tgcgacctat gtagctgagc atgctccgaa cggtcgccgt
    29341 ggtttcatga ccagctggat tcagatcacc gcgacactcg gcttgtttgt gtcacttggt
    29401 gtgatcttga ccactcgctc gatcatgggt gaggaggcgt ttgcggctac gggctggcgc
    29461 gttccattcc tgatctccat tttgttggtg gggctttcgc tttggatccg ttattccctg
    29521 cgtgaatcac cgttgtatgt caaactcaag gaaaagaagc aggtttccaa gaatccgctt
    29581 aaggaaagtt ttggaaatcc ttacaaccta agctttgtga ttctggcttt gtttggcgcc
    29641 accatggggc agggtgtggt ttggtacacc gggcagttct atgcattgtt ctatctacaa
    29701 aagatttttg gcgtgagcct tgtggattct aatttgatcg tgggtgcggc cctgttggca
    29761 gggacaccgc tcttcgtggt ggtcggtcat ctttctgata aatacgggcg aaagccgttc
    29821 atgatggcgg gaatgatcct ggcggttctc acttatctgc ccatttatca gacgatggcg
    29881 aaatacgctc cttttgttgg gaacgcggct gataaggtgg ttaacccggg atacagccca
    29941 ttcatgctgg gcgtgttggt tttcgttcaa gttgtgtatg tgaccctggt gtacggaccg
    30001 attgcagcct tccttgttga gttgttcccc accaaaatcc gctatacttc gatgtcgttg
    30061 ccttatcata tcggcaatgg tgtgttcggt ggacttgtgc cgatcattgg tctgtctttg
    30121 atccaatcca cgggcaataa ctttgcaggt ttgtggtacc cgatggtcgt tgcagggatt
    30181 tgcgctgtga tcggtttgtg gaaactgcca gaaacaaatc atattgatat cagttccgat
    30241 gcttcggttg catcgcttgg agcgaataag taaaagcttt taatcgtttt tggaagagac
    30301 agtcaccatt tcaggatatt cgccgaaatg gtgactgtcc gccgctaaag gagatggatg
    30361 ccatgaactt tatcagcccc accgttaatc gctggttaca ggaagcgaag gaaaaccccg
    30421 aagcattttg ggagcgagcc gccaaagaat tacattggtt caagacctgg gataaggttt
    30481 ttgaatggaa ttacccgaat ttcaaatggt tctccggtgc tctgacaaac cttgcttaca
    30541 acgcccttga tcatcatgta aagcgcggtt ggggcgggca cacggctctc gtgtacatga
    30601 acgagcgcgg tgaacgccag ttatttacat atgctcatat gctgtatgag gtcaagcgca
    30661 tggcggcggc tttgcgcggc atgggcatta aaaaagggga tcgtctcaca atttacatgc
    30721 caacctgccc tgaggcgatc atgttgatgt tggcaactgt ccgtattgga gcaattcatt
    30781 ccgtggtatt tgcggggttt ggcgagaatg cattgggtga caggattcag gccagtggtt
    30841 cgcgccttgt tttcacgaca gatattacct accgaaaagg gaaagatgtg ccgcttaagg
    30901 gcattgttga cggtgcgttg gctggcacga agaccgatgt ggaacacgtt attctgctaa
    30961 agcgcggaaa tgacgatgtg tccatgcagg atgggcggga cataacctgg caggaattta
    31021 ttaagtacgc tgaggggcaa aatggggatt gcacgccgat ggaggccaac gaacctgctt
    31081 tcattctggc tacttcggga acgaccgcca aacctaaact tgccatccat acccacggtg
    31141 gctatcaggt gcatatcttc agcatggcgc gctgggtcta tggcttgaaa cccaccgatg
    31201 tgtggtgggc gacttctgat attggctgga tcgttgggca tagctatatc gtttatgccc
    31261 ctttgattac aggctgtacg actctggctt ttgaaggtgc gttggaccat ccgcatgcag
    31321 aagcgaactg gagaattgcc atcgaagaat ttgcagtcag tggtgtgttc acctcaccga
    31381 ctgctgtgcg cctgctcatg cgttatggcg atgagccgtt aagtcgagtc aatcacgatt
    31441 accttgaaag aatattctgc gcgggcgaag ttctgaatgc gcctgcgtgg gattggctgc
    31501 aaaataaaat gctgggcaac aagatccctg tcattgacca tatgtggcag acggaaacag
    31561 gtgggccagt atttggaaat ccatatggga tcggcatgct gcccaccaag gcaggctccg
    31621 caaccattcc cttgcctggg attgaagtgg ctgtgttaac ccctgagggt gagccatgcg
    31681 cacccaacga aaaagggatt atggtcatca agcgaccctt ccccgggctc actcctgcac
    31741 tgtggggtga acctgaaaga tatggaaagg attactggtc taaaatcccg aacgtttact
    31801 acacgggcga ttcagctcat attgatgaag acggttatgt ctggtttgcg gggcgggcag
    31861 atgagatcat caagatcgca ggacaccgca tcggtaccat tgaagttgag actgccttcc
    31921 tcaagcatcc tgctgtggct gaatctggtg tgattgggca tcctgacgaa ttgcgcggcg
    31981 aggtcatttc tgcttttgtg ttgctcaaga atggtaaaac gccttctgct gaactcaaaa
    32041 aggaactgat tgataccgtc cgccatgagt tgggccccat cgcggtcatt ggtgagttga
    32101 attttgtcag catgttgcca aagacacgca gcggcaagat catgcggcgt gttttgaagg
    32161 ctgtcactct cgataaagac cctggcgata tcaccaccat tgaagacgaa ggctccgtaa
    32221 ctgaagcgcg gcaggcctgg cagcaaatga aggatgatat ttccaagggc gtgtaacttt
    32281 ggttgaactc tgcaaggtgt aaaattacgc aatttcaaga aagcggatta aaatagccgc
    32341 tacaattctt acctaactca ggaggctgtc atggcaaaaa aggaaatgga aggcgaagtc
    32401 tattatccat cagaagatgt gattgcacag gcgcgcttga aagattggga cgcgcttgct
    32461 gaaaaggctg gcaaggatct gcaggggttt tgggcagatg aggcatctga actggagtgg
    32521 ttccagaaat gggataaagt cctggatgat tccaataaac catttttcaa gtggtttgtc
    32581 ggtgcaaaaa ccaacattgt ccacaatgcc gttgatcgcc acctgaagac ccatcgtaag
    32641 aatcaactcg ctttgatctg ggaatcggag gacggtaagc aggagcgcac tttttcctat
    32701 tacgccatga atcgcgaagt ctcccgcatg gcaaacatca tcaaatccat gggcgtacag
    32761 aagggtgagc gcgttaccat ttacatgggg cgtgttcccg aaatcgtttt cgccatgctg
    32821 gcttgtgcca agatcggcgc cattcactcc gtggtgtttg gcggtttctc ggttgatgcg
    32881 ttgcagggtc gtattgacga tagccagtcg aaactggtca tcacctgtga tggttcctac
    32941 caaaatggaa agatcgttga actcaagaac atcgttgacg aatccatcaa gcgctgtccg
    33001 tcggtggaga atgtcattgt tgtcaagcga acaggccaac ccgtcgcgat ggaagcgggc
    33061 cgcgatcact ggtatcacga tctgtgtgcc ctgcccatcg ccaatggcaa atgcgctact
    33121 gaagtccttg acgctgaaga tccgctgttc attctttaca cctctggttc cactggaaaa
    33181 ccaaaagcca tcctgcatac gcatggcggt tacatggtcg gcacctattc caccatcaaa
    33241 tatgtctttg atgtcaaaga tgaagatcgc tggtggtgta cagccgaccc aggctggatc
    33301 accggccatt cctacattgt gtacggccca atgatcgctg gcgcaacttc aatgctgttc
    33361 gaaggcggcc ccaccttccc gtacccgaat cgctggtggc aggttgtgga acgctttggg
    33421 atcaccatct tctacactgc gccaaccgcc attcgtggac tcatgcgctt tggagaggcg
    33481 tggcccaaca agcacgatct ttcctccctt cgcctgctcg gttctgtcgg tgagccgatc
    33541 aaccccgaag catggaagtg gtattaccgc gtcattggca aggagaagtg tccgatcatt
    33601 gatacctggt ggcagacgga aacaggcgcg ttccaaatta ctccaacccc tgtggtgccg
    33661 ctcaagcctg gctcaggtac ccgcccgttc tttggccaga aggccgagat tgtggatgaa
    33721 caggggaatc ctgtccctga tgacaccgaa gggtatttga cccttttgaa tccgtggcct
    33781 gccatgctgc gcacgatcta tggtgatgac gaacgctatg tcaatcaata ctggtcgaag
    33841 tatcctggcc gttacaccac aggcgattct gccaaacgcg ataaggatgg ctacttctgg
    33901 atcattggtc gtgtggatga tgtcatcaag gtttccggtc accgcctcgg aaccgcggaa
    33961 gttgaatcgg cattggtcag tcaccctgct gtggcagaag cggctgcgat cggtttatcc
    34021 cacgaagtaa aaggacaggc gattcacacc tttgtgcttt tgcgctcagg tttctctccc
    34081 tcgcctgaat tggctgaaga actccgtcag cacgtggcca agcatatggg tccgattgcc
    34141 cgccctgaag atgtgaagtt cctcgataaa cttcccaaga cacgttcagg aaagatcatg
    34201 cgccgtgtgt tgaaggccag agcgcaggga ttaccggaag gcgatatttc aacgctggac
    34261 gagtagtaaa aaacgaaaca aaaaaacttc cgaagtttgt gcttcggaag tttttttgtt
    34321 tttgaaattt cttacttaac cagtttgagt ttccatgaat cgttggcgta agatgaagcc
    34381 tggtcgttga ggaagtagat accagtcatg cttccgtctt tgccgacatg tccgtaatac
    34441 cacccgccaa gggtataacc gccgcctgaa acgaatttgg tttcggtcca tttgatccat
    34501 atgccatccc gatctccatt ttggtaatcg aggtgtttgt tccacttggc ttgctctgtg
    34561 gcatcgccaa aatcggttac gaactcacct tcaataactg tgatcgcgcc cctgtaattt
    34621 gttccgccag tccagaccat tttcccggtg aaggtttgtc cattcatttt ttcgatcacg
    34681 atcgaaattg gtgtggaatt aaaaccagcc tgtctgtagg ttccattcca ctgttggggg
    34741 agaaggatcg gaacagccgt gttggtcggt gtgacctgtg gaatgggggt gtcgcttggt
    34801 ttcgctgttg gtgtatcttc tatctccagg gtggcagtgg gaacctctgg agaggtgaca
    34861 gccaatgcag tgggtgtttc ggcttctctt gcccacggac cgaaggagta ggccagccaa
    34921 catccaccgc ataggacgag caatccaacc gcgcccagaa aaagaagggc aatggatacc
    34981 atcggttttg acgatttagg ctgtcctgca tttgagatcg aagttgggga ggtcctttct
    35041 ctttgaggca cctctgcttg tttctgttct gcctgctttt gttcgatctg tgttttgact
    35101 tcctgaattt cctttttgga taccttgcca gtcatctgtt cacgcaaaat atcggttgca
    35161 actgcggttg cggtcacctg tccgccggta agaatgcttt gcaggtcatt ggcaaaggca
    35221 ttcatattgg catagcggtc cttggggttg cgggccagtg ttttgagcag tacagcttca
    35281 atatttgcag gcaggtctgg gatataggtt tttggaagtg gcagcggctc gttaacttgt
    35341 tgcagcaaaa tacctgctgg ggtgtcggag atataggggc ggcggcctgt gatcatttcg
    35401 tacagcacaa ccccgaggga atacatgtca gataaggcgg tcgtttcgcc cgtccattgt
    35461 tcgggggcca tgtaatcggg ggtgccaatg ccaacaccgg aatctgtcag ctgttgagaa
    35521 gtatcctggc cttcgaatag ttttacaagg ccgaaatctg tgagcatggc ttcgccgttt
    35581 tcggtcagca aaatattgga cggcttgaca tctcggttca tgatgttgtg gctgtgaacg
    35641 taatgcagtg cccgtgcaat gggaatgatg gtttcaacag cttcgcgcca agggatgggt
    35701 ttgccaagtt tttgcttgag ggtgccacca ggcagatagg tcattaccag gtaaggtgcg
    35761 ccatcatact cgccatagtc aatgaccccg acgatgttgg gatgcgaaag cctgcccaat
    35821 gccttggcct cacgttcaaa gcgcttgagg atcatctcca gttgatcggg tgggaaggct
    35881 tcacgtttgg tgaatttgat cgctacctca cgctccagcc tcgtatcata ggctttatag
    35941 acggttgcca ttccgccttc accgatcttc tcgaggatct cgtatcggtc tacttttttg
    36001 ccaattaatc catttcctgc catggcgttt tcctttttta tctggtgcgg ggtgaaatcc
    36061 gcctcaagtt gcatttgaat ggcgcaatta taagtcacat tcatcacaat tcactgtacc
    36121 ccaaattacc tattgttaaa acaaagacat ccgaaacctt tgtggcttcg gatgtctttg
    36181 tttggcggtc tatattcctc cgcgttcttt cgcaacagcg cggatctctg cttcgagctt
    36241 gtcagcttcc ttttcaagct cggccagttc atcgagcatt ttctccccga gagtacgatc
    36301 ttcgagtgaa ttgatgttga tgcggacatt acagcccgcc gcggtaattg aggcacgcgc
    36361 catggcaaat cctgacattg aatcgctgat ggcattcaca ttgccatgtt tggcgcattt
    36421 gagtgccagt cccatcactt tgacggcgtc ttctgacacg tgtaatggaa tgtgcgcggc
    36481 gttgagcgtg gcctgggcga tggcggcatt gcgtgtggct ttctgttcgt cggtatcttt
    36541 tggcagtttg aaagtggcca tcaatacttc gaaggaggcg gcgtcgtcat ctacagcctg
    36601 ggtgagttcc tggcgaaggt tttcggccac cacgcggatc gcctgcattt cggcttccac
    36661 gtcggcgtat tttttcttgc ctatggtcaa cccggcgacc atggcaacga ggccagcgcc
    36721 catggcacct gcgtaggccc ctgcagaacc gccgccgggg gttggtgtcg gggcggctaa
    36781 ttgttcgatg aaggaagcag gctgaggcgt ggaaagaccg tggcttgggg atgatgaact
    36841 gcctgtggtc tgaggtccgc tgtcgaagag tcgtgattcc aaaatctgtt ctttatcaaa
    36901 ttgatccagt tgtgtgtacc agaccgctgc atcaacaagc gcttcttcgg ggattaatcc
    36961 aaccagttca ctgtgatgga tgccaacacc ataccgttgg gcttcgcgtc ggatgaattc
    37021 tacgacccgc gcaatggggg tttcgcggaa gttggtgagg ttcatcgaaa cctgcgcgcg
    37081 gccatccacg agcaagccaa ggcctttgac atagcgcaag ccgcccgagg actggcgtac
    37141 agcctttgcg atcttttttg caatttccac atcttctgtg gtgagataaa cattgaaggc
    37201 aataagcgga ttgcgcgcgc cgatgacagt tgcgcctgcc ttgcccagtt ttgcaggacc
    37261 aaaatcaggg gcgcggtttg ggtcggtttc gatttctgtt ttgagtcctt cgtactggcc
    37321 tttgcggatg ttttcaagat tggttcgctc tggacgagtg gcagccgctt cgtataaata
    37381 gacggggatg ctgagctcgc ttccaacacg ctgacccaac ctttgggcaa tggcaataca
    37441 atcggccata ttggcacccc ccaatggcac gaagggacag acatctgttg cgccgatgcg
    37501 cggatgtgcg ccggtgtgct ggtcgagatc aattagttct gaagcggttt tgatggcgcg
    37561 aaaggcggct tcttccacgc ctgcggggga gcctgcaaag gtgaggacgg tgcggttgtg
    37621 gtcgaggtcg gaggagcggt cgagcaattt cacttcgctg accgattgaa tggcggcaac
    37681 gatctggtca atgatctcag gtcgtcgcgc ctcggagaag ttgggaatgc attcgatgag
    37741 taggttggtc atgggaggtc cgatttttga ttttcgattt tggatttacg atttacaatt
    37801 gcatgggtaa ttataaagga tgaaagttga tgaaaacaaa tcaaatgatg gctgacaatc
    37861 ttcccgaaca tttaaacaag ctatctcccc gtgagattga agttgttgac catgttctac
    37921 agggcaaaag caacaagcag atcgccctcc aacttggcat cacagagcgg acggttgaat
    37981 ttcacctgaa gaatgtgtac accaaactgg gaattggctc gcgcatggaa ttggcggtca
    38041 agctgtggaa atccgcagat gatgaagtga aagcctccct gagggaatcc acagtagcgg
    38101 aaagtgtgga aattgcggaa aatggcaatc agcccatgtg ggcggcatct gtaaaaagga
    38161 tgttatccgt tcttaaaaag gagtttgcca tgaccatgaa aacaattctc aacgaccttg
    38221 gaaatgccgt tcagaaacat cttcttgcct tcagtttgtt ctatttcatc gcagcagggt
    38281 tgactaccca ctatcttgta gtgggctttg gcttgtattt cataccttca tatttactgc
    38341 tgggacttgt cctcggcatg tcgagcttgt atctcggttt gcgctggcac ggaatcaagt
    38401 ctggggaggt gcatgtccgc ccgatttggg ctgtagggtt tctggccctg cctttccttg
    38461 ctggcctatt ggacttgacc cttttggcga cactggcaaa ggcaatgggg gaggtgacgg
    38521 tcaacttgat cgggctttcg agccgcgcag tgtggatggt tgccgataac ggcagtccat
    38581 ttttgttatt gagccgttcg atcacgctgg atgatatctg gctatatggc cccgttttgt
    38641 atatgtccct tttggctctg gcaggacgtt tctacacaat ccgaatacta aaacgaacag
    38701 acgccgtata aaatgacctt gcaacagatg gcaggcaata gtctgccatc tgtgtttgtg
    38761 tgccattgga atgcagcaaa ctctgcctac ggccgaaaat ggacagtcgc tgtcccagcc
    38821 cgtataatca gcacactatg gaaaaatccc gtttggaagc ctttagcgat ggtgtgtttg
    38881 ccatcgtgat taccttgttg attctcgata tccgtttccc tgaagtggaa tatgctcaat
    38941 tttcggagac attggtttct gtcgttccgc gcattttagc gtatatcatg agttttgtga
    39001 tcatcgggtt gtattgggtc atccaccaca actccatgca tgcgatcaaa aagacagacc
    39061 gcggatttct ttggctgaat atcctgcttt tgttgtgtgt gagttttatt cctttcccaa
    39121 cttctctggt ggggcggtat ccctttcagg cggggccgat cgtgttctat ggctttaccc
    39181 tgatcgcctg caatgcggtt ggatacatca tgctgcttta tgtctattat catccgcatt
    39241 tggcggttgc tgaattcagc aaacaatata tgcgcagcca cacgccgaat tatgtcatcg
    39301 tcaatggggc atatttgggt gcgatcttgt tggcaaattc gtttccactg gtgagttatt
    39361 tgatctatat tgccgtggtt attctgctga tcgtatttct tccaaaattg gatgatggga
    39421 tcaagtatca tgtagagtaa gtatcaatcc ctttgcgatt ttcgtgtagc caataaatta
    39481 ctaaatattg tttggtggga gagtttgtga taaaaaatct tgcaaataag ttacacacat
    39541 ttaccttggc tcttttgcgg ctggggttat cttttctttt ttcgtaaagt tgctggtttc
    39601 gatttgatat ggcgtgtgaa taaggagtga acgatgcgag cgtttctgat tcaatctgtt
    39661 gtagctctgg tctttggatt tgttttgggg gtgattggtc tcctggtcgg tgcatggttt
    39721 ggcggaaatt ttgcggccag ttttgagttc aacggtgtgc gcggatatga ggctacgggt
    39781 caggttgggt ttattttgtt tgccctggcg ggtctgattg caggctggaa attcatggag
    39841 aagcgaacga agaaacttcg ctgatttttc ctattatgtc tttaatcctt ttcgattttg
    39901 atggtgtcct tgcggacacg ttgggcgatt tgcttcaatt tgggcaggag gtttgcgatg
    39961 aattaggggt gcagcacatc gcaactcagg aggacttgaa ctcccttgaa gtcatgtctt
    40021 ttgccactta tggaattcag ctggaagtcc ctgcgcattt ggtggatgaa tttgtgcggc
    40081 gttgtttgag ccgatttgca gagaaaaagt caccgcctgc tatttttgat ggattggcag
    40141 aggttgtgcg caagctggca aaggagaacg tccttgccgt tgtgactggg aattcaacac
    40201 aaaacgtgaa tgcatttcta gcggagcatg gcattctaga ttgcttccgc gcagtgtatg
    40261 gggtggattt gcctggttcc aaggtgcaga aaatttccct tgcacgggag cagttttcgg
    40321 cgggaatgat gcgcgagtct gtttttatgg tgggcgattc gttgagcgat atccgcgcgg
    40381 cgaaggaggc gggcatccaa agcattgctg tagcgtgggg gcatcagagc ctggacaggt
    40441 tgttgagcgg cgatcctgat catgttgtac ggtcagtgca ggagcttttg acattgtttg
    40501 ctgtataatc gcgcgcctat gaatacaaac tcctttgatc ttgtcatctt cgattgtgat
    40561 ggcgtgctgg tggatagtga gccgcttgcc aataaagtgt atgtgcagat gctggctgaa
    40621 cttggccacc accttgatta tgatgcaacc ttgcgtgagt tttccggggc ggcggttttc
    40681 aaacgtattg aagccacatc gaaaaaattg aactggacgc cgcctgtaaa ttttttgcct
    40741 gaattctttt cgcgtctttc ggaattatct ttgcgggaat tgaagcctgt ggatgggatt
    40801 cgccaactcg ttgagagttt aaccgtccca gtctgtgtcg cttccaatgg cgcccgtgat
    40861 gagatcgtgc ttcgattgaa acttgccaac ctgacggagt tcttcggcaa tgccatcttc
    40921 agcggattgg aagtgccgca tcccaaaccc gctccggatg tgttcctggc tgcggcgcag
    40981 gcatttcacg tgccgccttc acgatgtatt gttattgaag atagcgttcc tggtgttacg
    41041 gcggcggttc gagcaggaat gcgggtgtac ggtcatgctg cgatcacacc tgtagaggtt
    41101 ctgcgtgaag ccggtgcaat gcctttcagg gatatgcgcg aattaaagcg gattctttct
    41161 gcacaggggg tatgattgcc cgtcgcaacg cttgatcaca tggatcaatt aattgaagtg
    41221 ctggggctgt ggatttcacc tatggaatgg tgtatgatga ttaaaatcaa atttccgctt
    41281 ggagagattt atgaccctac ctcctgaaga agtcatttta aaaggtgcag ccgcgtggaa
    41341 taaatggcag gaagagaata agttcccgcc ccattttgcg cgtccatcct ggtacgagtg
    41401 ccgcgatgag cgcggcatgc aaatcaaagg acgaaatgag cttgatttca gcggtattca
    41461 tctgaccaat gcctcggtcc acctcgcgtt tgccgagggg ttgaacatgc ggaatgcgga
    41521 agttgttggc tgtcattttg aagaagggga tttctcacgg gcggattttt ccaatacaac
    41581 ctttgtcaat acgaaattta ataagacgat cctgacggac gccgtttttg aaggcgctac
    41641 gtttatcaat tgcaatctca accgcgtgaa ccttgtcaat gcagatttcc gactcaagga
    41701 gatacgggaa actgttgtgt acggcatatc tgcatgggat ctgaaaaccg accctgcaat
    41761 gaagcaatcg agattggtga ttgagaaatc gtatgagctg tattccgatc ttattgccag
    41821 tggaaagatc cccatgatgg tggatgacat tgaaatggcg cagttcgttt attacctgtc
    41881 gagtcataag aacatgcgta atatgatcaa catcttgaac tccaagggag ttttgctcct
    41941 tggtaaattc aaagatggcg gtctcgaacg tctgtacagc ctgatggatt ggttcaagac
    42001 cagaaattac acacccatgc tgttcgattt tgaaagaccc aatagcctcg attacaccga
    42061 aacggtcatt accatggcgg ggctggcaaa atttatcgtg gccgaccttt cgggtggttc
    42121 tgttcctcag gagttgtacg caaccctttc aagttttcaa aagccagtca tcgtttattc
    42181 ggaccagcct gcctacagca tgttcaagga cattaagcgt aagaaccgtt tttcctttga
    42241 gatcgttttc tctgatgaaa aagatctgta tgcaaagatg gaacacagcg taaaagaagc
    42301 ggaattgggc catgcccaac tcgcccatga cctgatcgat tcgcatggtt aaggcggatg
    42361 ctggtcggga aatgaaatag gaataatagg gcagtcacca aatcggtggc tgcccttctt
    42421 tttatgtaca attacaggtg tcgtagaaac attgaatgga attcaaaagg agaagcgatg
    42481 agaatctaca aatattgggc ggttgaacgc cagaccttcg aagttcaggg ggaacagaag
    42541 acaggcactt tttatggagg ctcgaacgag tcggtggaag atgcccgggc gaaggttcaa
    42601 gccaaattag acagagtcaa gcgtaagatt gcgggggatc cgcgtgcatt taatggatat
    42661 gaagtagata tccgcgaaca ggtccttgag gagattaatc cgcaggcggt gatcacccgt
    42721 aaccgatacg gggcgaaggt gctcaatgtg gcgagcatga tgattttgga tatcgacaaa
    42781 ccaaaactgt cacttagtgg tttgttctcg aaaaaagatg ataaatcgaa aatgtatgaa
    42841 atggttgaga aactggcgtc ttcaaaatat tcccagtttg gtttccgcat ctatgaaact
    42901 tttcaagggg tgcgggtgat cgtgctgggc agggattttg acccgcggaa cgcggtgacc
    42961 accgccatga tggccgagtt caactgtgac ccgctttaca ccatcctgtg ttcgaagcag
    43021 gaatgtttcc gcgcacggct gaccccaaaa ccaagacgaa tgggaatgaa gtccatgacc
    43081 atccgttttc cacgcgaaga tactgacctg caaacagaaa actggttaag tgagtacgac
    43141 cgtcggagcg gtgatttcag cgtttgccgt tttattgggc aggcgggtgc ggctgcgcca
    43201 accaacgacc ttgtccattt gcacgatgaa ctgacaggcg cttccattgg gcggcagctg
    43261 gcttaagaac agaggtctct atgaatatgc aggatattcc ctttgggaca acgaattggg
    43321 gtgagatcgc accaacggaa cacaaaggta taacgggcat ggcgctttgg cgtacacaac
    43381 agtttggaaa catccgagtg cgtatggtgg agtatagcgc tgggtatcat gccgatcact
    43441 ggtgtgaaaa gggccatatt ttgttgtgcc ttgaaggaga attgcacacc gaactggccg
    43501 atggaaggaa attcaccctc accccaggca ccagttatca ggttagcgat ggaactgacc
    43561 cacatcgatc cttcacagaa acgggcgcgc gattgttcat cgtcgattga acatagattt
    43621 ccataaaaga tgtgattgtc agtcggttat gttttcgttt aattcaacgc ccatttcctg
    43681 catgtctttg aatagtctca aaggttcggc gatatgtccc atcatgtgaa ccccatcacg
    43741 gcggacactt ccatcaaggt attgtttcaa aaacgcgacg actggaatcg cagtgaattc
    43801 atatccgtct ggatgtgcga ttcttacatg gacctcaacc tgtctgccgt ttaacaatcc
    43861 ttttgcttcc gctttgagcg ccaccacata cggcggtttg gattttccca tgctccacca
    43921 catcaatttc ccgagtggat gaagcccgcg tttgggtgcg agtttgagac ccacaaaaat
    43981 gatcggtgta atgacaaggt ctgaaagcca gtttgatcct gcaatataaa aaccagtttc
    44041 cttaagcgag ggatatagtt ttgggatgcc ccgcagttct tcaaaaaaca tggaatatgc
    44101 ggttcgtttg ccgatgccat cgccgaaatg aaagggacgc gaatcccatg cggcaggctt
    44161 ggtccatgag ccgtttttat aaacctgtgc ttgataattg atgaaacctt ccatgagttc
    44221 atctacagct tcggtgtagg ggagttcctt catgttgaga tacccaccga tcaaggcaga
    44281 ttcgagtaca tccaattttt tagaggcata gcgaatcaat gcggaaggta agccagggtg
    44341 gaatcctgct tcggtgacga aacataaacc tgcctgctcg atctcttttt cggcagcgaa
    44401 caagacctca agctttcttg aagaatattg cacgtccaga taatccacgc gcgcttgaat
    44461 acaggcgcgg accactgtct cggcgtggtg tgtcgtcggc gcagcgacca gacataatgt
    44521 aatgccttga agcgcttgca aaaggctgtc gaaactggcg gcatccactt gaatcgcggt
    44581 tgcgcgtgag ttgttcaatt cgctggtgaa atgtttcgct tttcccaaac ttcttccagc
    44641 gatcatgatt tggacattcg tttgagctaa caaatatttg gcaagcaatt tgcctgtata
    44701 tccatacccg cccagtatca aaatcttttc catgatgttt actctttttc aggttttacc
    44761 gccagtaggg tcaggttttt tccgagcagg cggagttctt cttcgtcttt gaatttcatc
    44821 cctgccatgg ccatgacaga acgggtgtag gcgggcttgg ctcgataaaa ttgtacaagc
    44881 atttccccgg caccttccgt ggaaagcgcg cctgcctgtg ccttgaactt cctgcgccca
    44941 acccttgcct caaaaatggg attgctttga atgtttttgt accaatcggc gtgcgcgcca
    45001 taagcggcct cgatatagta ggtgtctgtg ttcctgttgt agtccattac gtccaccatc
    45061 acgtcgcggc gtttccccgt ttttcttcca atggtggtga tgagcatcca ttgggcgccg
    45121 atcaggcgtt cccagccgcc caatcccatt ttatgcagcc agacggggac cttgaatagg
    45181 aatttttgta attggtcggg tttgtttttg agcatgccca tgacttaatt ccctgtgatc
    45241 aaaaacttga agaacttccc aatgtcccgt ggctttgccc aaaaggctgg gacatgtttg
    45301 cggtagtcaa gatacggttt gccaaagcgt tcgagcaatt ctttttcttc cacaaggcga
    45361 atccagcctg tcaaccccag cggtacaaat actgaaaaaa tgaccgagtt gccgttgcca
    45421 tttaacaaag ccagcccaac ccacacacgg atggcgctgg cgtagatcgg atggcgtaag
    45481 atggcataaa tgcttgaatc tacgatacgg ctttcttcgg gatggtaaac atagagcagg
    45541 ttgagattgt ccacgccaaa tgtggcgatg cttcttatcc aaagtattgt gccgaccaaa
    45601 acccaatacc agccgaggac ggtgaagacg gtggtccacc agcctgtggg gatcacagga
    45661 ccgttcatgt atcctgcatg agcgaccgcg ccaaagatca ggcataaccc aggcagggcg
    45721 taatgtgcaa aggcattacg gtatgcgagg tctttatact tctcttgata aacaggtttg
    45781 cgggagaaaa acaagcttaa gatcaaaaat cccagcgagg caataatgat ctcgctatca
    45841 atactccagg tggggatgtt gtcagttacg atgaaataag ccgtgacaag agccagcagg
    45901 gacacaacgt agagaacgat ccgcgttctg ccgctggttg aattcagttc agggacatgt
    45961 ttggagagcg aatccaagcc tttgagtttc atggtcacct cttggaaata gtatttcttt
    46021 ttgcgggtga agtcaagcgc catgttgtga aaatgaaaca ggacgccagc cagctaaaga
    46081 acggttgtct ttgcgtttgt ttggcgtcct gtgatcggaa gttgttgcta ttttcttaat
    46141 ccgcggcgcg gatgcgtaac tcttccgggg aggggatttt ccctcgtagc gcatcaaatg
    46201 tcaggtcgct tgaggcgcgc ccttgtcggg tgttgtaatc ctcaaaaatg cgtgcatatc
    46261 ctgcgccgac ggcggcttcg atgttcaatt ccagccgttt ggacattgcg cggatgcccg
    46321 ctccgtttgc gccttccact tccacaaaat cgccgtaggg taattcgtcg agcatgatgt
    46381 ggtagccgtc gatttcatac acagcgcgga atttttcata ggtcaggatt tttacgtatc
    46441 ccaatgcaat gaggatcttg tgtgctgttt cgaaatcgcc gatctgggtt tccaattctt
    46501 ggcgtgaaaa aacgccatcc accagatcgc ttgggccttt gtaggtgatg gtggcgacgg
    46561 catcctgcct gatgcgcaac acccgtccgt tcgtgcgcat actgccatct ggcaggtcat
    46621 agcggaaatt gatttcatgc acacgtggtt gaatcaattg cgccttcaat tcgagaaggc
    46681 gcaattcgac ctttttgagg tcacgcacaa aaaattttac ttcggtttcc tgttcgccca
    46741 tttaatgcac gctcaacatg gcttgctttt gttccacgct ttcgcctgct ttcacacgaa
    46801 tgcggccgat cgtgccatcg cgcggagcct tgagctcgtt ctgcatcttc atcgattcga
    46861 ggatgagcaa gacctgaccc ttctttacgt cttgtccctc ttctacgagg atggcaacca
    46921 caaggcctgg cattggcgcc ttgagatgga attctccacc ttcggcaatt cctccacctg
    46981 cagcggctct gagacgtttc tcgcgctcat cctccacctt gacctgatac tgtctgccgc
    47041 gcagtaacac atcccaatct tcttcgccct gataaacgaa cgactcgtag gaatgtccat
    47101 cgagaagcag agagaatacg ggctgtccgc tgaccgattc aaaatccact tgcagcaagc
    47161 ggccgccaat gcgaatatgg cgctcgtcca ccacttcgat ctcaaattgc tggtcgtcaa
    47221 ccgttgtaat atatttcatc tcgtatcatc ctttagcggt gcatgcgttc ccagcggcca
    47281 acccatttcc agttgcttgt gtcgcgttca ttgcggcgca ccacctgtgc agaccgctcg
    47341 ctttgctggt gttcaacgag tgtggccaga atggcggcga tctctgcgct ggaggcgcgg
    47401 gcttcgacgg cgtcgtccat gctgaagcgt tcttccacga aacgggtatc gaactggcca
    47461 gccatgaagc gatgcgaatc catcagtgtt tggtggaagg gaatattcgt gcgtactccc
    47521 acgatgcgat attcttcaag ggcgcggcgc atgcgcaaaa tggcctgcgc gcgagtttcg
    47581 ccccacacaa tgagttttgc gatcatcggg tcgtagaagg gggtgatttc aaagccaggg
    47641 tacacacccg tatccacgcg cacgccaggg ccagtgggga tcaggctgtg ggtgatgcgc
    47701 ccagtggaag gcaggaatcc gttgaatggg tcttcggcgt tgatgcggca ttcaatggca
    47761 tgaccgttga atttgatctg gtcctgggtg tagcttaatt gtcgtccgcg ggcgatgcgg
    47821 atctgctcgg ccacgatgtc caccccagtc accatttcag tgatgggatg ctccacttgc
    47881 aggcgggtgt tcatttcaag gaagtagaaa ttgcggtctt tatccacaag gaattcgatc
    47941 gtgcctgcgt tgatatagtc aacggcttgt gcagatttca cagcgatgtt gcccattttg
    48001 gtgcgcaggt catcgtcaag gtaggacgag ggtgcctctt ccaaaagctt ttggtggcgg
    48061 cgttgaatgg aacattcgcg ctcgccgagg tgaatgacgt tgccaaatga atctgcgatg
    48121 atctggaatt cgatgtggcg tgcgccttcg atgagttttt ccagataaac attgccgtcg
    48181 ccgaaagctg actctgcctc gcgtctcgcg gactggagca gggtcggcat ttcctcaatg
    48241 ctgcggactt cgcgcatgcc ttttccgccg ccaccagccg tcgctttgat cagaagcgga
    48301 aagccgatcg tgggggcgat gcgcaggagg tcgtcatcgg tcatgttgcc gacatcttcc
    48361 gtgccaggca caacgggcac gcccgctttg atgacggttg cgcgggcctg gcctttatct
    48421 cccatggctg caatggacga tggtttgggg ccaatgaatt tgatgcccag ttcggcgcat
    48481 ttttcagcga agtcttcgcg ttcggcgagg aatccgtagc cagggtgaat ggcatctgcg
    48541 ccagattttt tggcgacatc gaaaatttta tcggcgcgca aataagattc gcgggaaggt
    48601 gcggggccga tcaaataggc ttcgtcggcg tagcgcacgt gcaatgcgtt gcggtctgct
    48661 tcagaaaaaa cagccactgt ttcgaggccg agttcacggc aggcacgaat gatgcggacg
    48721 gcgatctctc cgcggtttgc aacaaggact tttttgaaca tgatgtctcc ctgttccttt
    48781 acagcggaat gttgccgtgt ttcttggcgg ggttggcatc gcgcttgttg ctcaacattt
    48841 caagcgcatt gatcaaacgc ggacgggttt ccttcggctc gatcacatcg tcaatgtaac
    48901 cgcgctgcgc ggccacatag ggattggcga atttttcctt gtattccgca acgagttcgg
    48961 ctttcttttg cacggggtcg gctgcggcat cgagttcctt gcggaagatg atgttcaccg
    49021 ctccgtcggg gcccatcacg gcgatctcag ccgtgggcca ggcgaggttt acatcgctgc
    49081 gaatatgctt ggatgacatc acgcagtatg cgccgccgta tgctttgcgg gtaatgaccg
    49141 tcaatttcgg aacggtcgct tcgcagtagg catacaacag ttttgcgcca gagcggatga
    49201 tgccgtgatg ttcctggaag gtgccaggca ggaagccagg gacatcttca aaagtgatga
    49261 tgggaatgtt gaacgcatcg caaaagcgga cgaaacgggc ggccttttca ctggcgtcaa
    49321 tatccagcac gccagccaat actgcgggtt ggttggcaat gatgccaaca gagtggccgc
    49381 ctaaacgggc aaaaccaacc accacgttct gggcgtagtt ctcgtggatc tcgtaaaact
    49441 gtccgctatc catgatcatg cggatgactt ctttgatgtc gtagggcttg ttggcgtcat
    49501 cgggaatgat cgagttcaac gcttcgtcca tgcgcagtgg gtcgtcactt tgcacgaagg
    49561 gggcatcttc catgttgttc tgcggtagat agcccaacaa cttgcggatg aggtacaagg
    49621 tatccgcttc actgtccgcg gctacgtggc acacgccaga tttttcagag tgaacacctg
    49681 cgccgccgag ttcttcctgt gtcacttctt cgtgggtcac agccttcact acatcaggcc
    49741 cagtgacgaa catgtaggag gtgttgcgcg acatgaaaat aaaatcggtc agggcgggcg
    49801 agtacaccgc gccgcctgca cacggcccca tgatggcaga tatctgcggg atgactcccg
    49861 aagccatcgt gttgcgcagg aagatatcgg catagccgcc aagggctaca acaccttcct
    49921 gaatgcgcgc gccgcctgaa tcgttcaggc cgatcatggg cgcgccattc ttcatggcca
    49981 tatccatgat cttgcagatc ttttcggcat gtacttcgcc aaggctcccg ccgaagacgg
    50041 tgaaatcctg cgagtacaca tagacgagac gaccttcgat ggtgccccag cctgttacaa
    50101 ccgagtcact catgaatttt tgctcgtcca acccgaaatc atgtgtgcgg tggacgacga
    50161 atgggtccac ttcgcggaag gagcctttgt ccagtaacag gtcaaggcgt tcgcgggcgg
    50221 ttaatcgtcc ctttttgtgc tgtgcgtcaa tgcgagcctg gccgccgcca aggcgggatt
    50281 gtgctttcag ctcagtcaat ttttggattt tcgatgtttc gctcataggc ctctctcgtc
    50341 agtgattttt ttgaaaacag gataaaaaca atgattgcca gatttactat aacagcgaac
    50401 aaccaatttg ggtgcggatt ttgccaggcg agacgctcgg tccaatacca caagctgtag
    50461 cctgctgaag cgccaaggag cagtttttcg gcccaggcag ttttctgcca aatgccccac
    50521 aacaggacga ttccgcacag ggcccaaacc gcgccgctga ctgccaaaac aatgggggat
    50581 ggctgcgtgg agaactcggt caggatgtcc tgccaggcaa gggctgtcca catccgcagg
    50641 agattgaatg ctgttagggt taacaccagc atctggagca gggttgctcg aaaggaaaac
    50701 ttcttcatcg ggtgaaaatt gcctttggaa cagaatcaga ccctgggtct gggtaaagat
    50761 tcggaaagtc catccaacag aaaggtatgg atatttgcat cggttaatat tttggcttat
    50821 ccaaaagata caacaaaccc ttgccgccat actcgctgta gctttcgagg tatggattga
    50881 attgaccgaa atacggcggc aattccagca ggcgcggact ggcaaagaca atggtcatgc
    50941 catgttcgcg caggtaggca ggagcctcgc cattttgcag ggcatggaaa tattcgtagc
    51001 tgttgatgag gccgtccatg ttgacgatgg ttcggtcgtg aataaagtag ccgacattgc
    51061 cgccgcccgt catgccgatg atgctgcctg gtggggtgtt ttcttcaaga tatggcagca
    51121 catccatgta ggggcgttca ggtggaaaga agttatacgg catgatcttg accacctgtg
    51181 cgccgaagcg ataagcaagg agcaggcagg ccgcagctga aacatattga atggcgagtt
    51241 gcgccttcgt tctcaacagc gggcgaatca aaaagtccaa cacaacactg ccaagcaggg
    51301 tgacgaaaac catctggctg acccagtacc attccttcgc gccgccgtac gcagtggtgg
    51361 tgtacgcaag gatctgaatt ccacaccctg ccatgagggg taaaagagcc atgtttgaaa
    51421 acgcccgccg tgtgcggttg gcgttgacga aaagcgtgat cactgccacg gcaaccaaaa
    51481 gcgcgagaaa cgtgtaagag aggtcactca cgatggggat atctgcgaac ttcggaaagt
    51541 tgaccttgac cagccacaag aataaacccg atgcaggctg ccaggcatca taagcgcctt
    51601 gaaaactgag accgaaaaag gccgtccaat ttggggcagg gctgtcatat acggtttcgc
    51661 caagcgtacc ccaccagcgt ttgacctgtc cgctgaccgg gctggaggtt ccgaaaacaa
    51721 ggcgattgaa aagcatgtaa agcaataacg ctccaccgac gatcccataa taaggaatgg
    51781 cttcggtgaa ccatgttttc cagttgcttt ggaattcctt gaggggagca ttgttgaata
    51841 tgcttttttc ttttttgtct gtgttgttct gcggattgat tgctctgttt ccgaaccagt
    51901 aacccgccag cctgagcgcg aggatgagga aggcgctcaa accaaggtca atgaggaagg
    51961 cactgcgtgg gaagtccctg ccgatgccga gttgggcgag cagtaaatac aaccccgtaa
    52021 gggccatggt gctgaaagtt aatgcaaggc ctgcttgaag aagggtcttc acgaccgagt
    52081 tttcgcgtgg atgttgatat gcgccaaaaa agtacagcgc gatgatcttc actgccaatg
    52141 ccagtacgac cgcttgcacc gctgaagctg caaacatgga attgtacaag gcgatgccag
    52201 ttcgaagcgc aactgaagtg gtcatcgaaa gaaagatgat gacgacatca agcggaagca
    52261 gaaaacgcat gggcttgccg cgcaggatga tccaaaagcc gccgagaatg gcgaggaaga
    52321 ccaggtcgag gcggctgagc atgacgaggg ttgcaacacc tgccagattg agccattgtc
    52381 tttttgtcac agcctgggtt cgccagtctt tttcaagctg tgaaagtttg tagatgaaca
    52441 acacaaccgc gaatgccgcc agcggtgttt ccaacccgaa ttcatacacg ttattgtgaa
    52501 tgtagaaatt gaacgcccaa aacgaagccg ccagcatggc cactgcattc gagagattgc
    52561 tttttaccaa cctgtatatc aagatcgctg tggccgcatt aaagcctgcc attaccaaaa
    52621 gcatcacccg cagcggcagg atcaagtcaa aacgggcaag agtgaaaatc ggaatgcaga
    52681 tgagcatcca caaagggtga tagccgtttg tcaggttgat accatcgaac gtgatgccat
    52741 gtccctcggt gatgttctgt gcaaccttga aatagtaata ggcatcatca cgtgtgaacc
    52801 atgaattcgg caggttgtat gcatctgaaa atgccgcgta gatatgaatg cctagaataa
    52861 ccgcgatcaa cacggcttca aaggaaggga gacgttttag aaaattcatt tatggaatat
    52921 caagggtgat cttgccaaag ttttcaccgt tccacaggcg ttcctgtgcg gcggcggctt
    52981 ccttgagcgg gaaagtcctg tcaatgatgg gttggaggcg gccatccacg accagatcca
    53041 tcacttcggc gaaatcggca agtgtgctca tggttgagcc gatgatcgaa agatgtttgg
    53101 caaaaatata acggttgtca atttcaaaac gaggcgcgcc gctgttgccc actgtcagca
    53161 gccttccacc cttgcgtaaa attcgcaggc tcagtgggaa ggttgtgccg acattatcca
    53221 caaccgcatc cacaccgcgc cgttgggtgg caataaaaac ggctttcgac caatcttcct
    53281 cttttgaacg gtcaatcaaa atatccgcac ccagcgcttc ggcttctttt aattttttcg
    53341 aatttgatcc aatgacgatg actcgtgcgc caagatattt tgcaacctgg atactggcgg
    53401 aatttacgcc tccacccgcg ccgacaattg ccactgtttc cccaaccttt actcccccgc
    53461 gcttgaccat cgaatgccag gcggtctggt acaccaaagc cgccgcagca gcctgatgaa
    53521 ggttgaaatc ctcgggcagt ttgtatagct ggcgggcggg caggcagata tattcagtgt
    53581 aggtgccacg cacactctcg ccaagcaaat gccagttgag acacatgttg tcctgccctt
    53641 tcagacagaa ctcgcaatcg ccacatccaa ggttggcgtt aacgacgacg cgttcgccga
    53701 tcttgaatcc ttttgtattt tcgcccaggc ctgccacttc accagcacca tctgcgccgt
    53761 tgatatgggg aagttcaagt ttcaacccgg gccagccatt gcgaaccatc acatccatac
    53821 ggttcaaggc agcagccttc aagcggacta ggacttcgcc atttttcggc tcaggcatgg
    53881 ggaaatcggt gtattccaat acttccggtc cgccgtgttt gtggaagaga attgttttca
    53941 taatgtttca ctcattttat tcgatgtttt gccagtcaac cactgcattg caaagagaaa
    54001 tatggagccc actccgcaaa tgagcagaat cgaacgggct ggtgaattcc aaaggtctgt
    54061 tataccatgc tggaggctga tgtccatact ggtttttagg ggaaggtcgc tgatgacata
    54121 tccgcgtagg cgctggcccg gggcgtatgc cgcttgtacg tccgctccgg ggatttgggc
    54181 ggccagttct tcagcatagt ccgccccgct ttgcggacta ataaaggcca caaaattttt
    54241 ccatgttgat acttgttcag ggttcacact tatttctgcg gatgacaaca ttgtggttgt
    54301 gtgtgggctt aggctgtctt gaacgggctc gacctggttg tcaatgatgt acaggtggat
    54361 gggttcttga atttggcgtg taagccatgc aatatagtcg ttttgatttg gcggcgcgta
    54421 ggcgaaaggg agtagaaaaa aatattgtac acaacccatc aacacgatga tggacaaaat
    54481 gccagccgtg gtcagcatga tgcttcgttc tttgattgta ttcagcacgg ttgttgtgaa
    54541 attagttaat ccactggcag agatcatcgc aatggcaggc agaatcgcca ccatgtgtgt
    54601 tgggcgtggc gggattgagg caagcacacc cagaaaaaag aatcctgcca gaaaaaaaat
    54661 aagcataatc agatggcgta gtttttttag gtcaaccaat gccatgccaa accccaatgc
    54721 aaaaaaaatg gatgagcctg ggccagccag accaaaaacg atggggtggt cgttgtaacc
    54781 gattgggtcg aataacacag ctgtggtgcg gataaatccg cgcgtgaaca cgagtttgta
    54841 taaggaagcg tcgtagaaaa gatcaacccc ttcaaggttg aagatatcgg cttccaccat
    54901 gcgttcatcg ccgaagacca gttttccata aaacaggttg atggggccag tttcccagat
    54961 tttgtaggtt aatgattttt cgttttcacc gcttgcgccg tacaaaactc tcggcaggat
    55021 gacgaggcat gctgctgttg agacgaccaa tagcgaagtg atgccgctcc gaaatgactg
    55081 gcgtgaaatg agtgtaaaag caaggatgat aagaattgcg gcggcaagtc ccagccaggc
    55141 ggcgaaataa gtgtagaaac caacccctgc agccaaacca gccagccaga aataatagag
    55201 gctgtttttc ttcagcccaa gtgttaggaa ataaaggcac agggtgacag gtatcaaagc
    55261 ctgaatgtta ttgtatccaa gccgggcaaa ggcaaggaag tatggattgg cgatcatcat
    55321 aaaacttgcc gcgatggctg tgcggcggtc aaatagctca cgggtaagca aataaagtgg
    55381 aatgaccgac cctgccgcgg gtagtacaga agcgaagcgc catccccaca tgttgatgcc
    55441 tgcccaacgc atgatccatc cctgtagaag cgagcttgcg atcggaaagg taaatacccc
    55501 tgtgtcgaaa aaggacggat cggttttacc gatggcgatg tttcgtgccg caacccagaa
    55561 tggaccttca tcggcgatcc agccagcggg gaggtcattg agcagatagg cgcccatggc
    55621 tgtggcaagt gtgaatagac ccagcatcca tgccgcatcg gtgagggtga tccattccat
    55681 gggtttatgc tcgttttccc aaaaaaggat ggtgaacaca agcaggaatg ccagccaacc
    55741 ccaaaagaat gtgaccgaat ctttgtgaag ggcaagctgg accatgatga acccgtacac
    55801 gattaaattg agaatgaccc agtgtttgta ccgtttccat ttttcccagt gcatggtgcc
    55861 catgacgggg taatcctcag ctggggcggt tcttgttgat aaagccgaga ctatggtgcc
    55921 aagtagaatg agagcgaggc cgatcaatgt gttgaaagga ttgctcaggg caagatatgc
    55981 atcatcgttc cagtttttta gcgtgtcagc cagtgtgtcg aaatacggga atggctgtgg
    56041 ttccctcatc aaggcttggc cgagaacgac cagaattaaa gcaaacacca gccaatattt
    56101 tcgaggtatt ggctggtgtg aatggatatc cattgtcgtt gaggtgaatt tcatcctgct
    56161 gaaatgcgcc tggggtcagt ccaattgttc acggtggaca aaaaagagca gggcgaatat
    56221 tgtcagcagg gtaatggtca ggccgatctt atggagtggc gggtcaaaga taaatgtgta
    56281 agtgtgtttg ccgggcaggg caggggcggc aaggtattca ttggccgggg taagcggaac
    56341 aggttcacca tcacggagaa ccttccagcc ggggtagttg ttgaccaatg ccaccaccac
    56401 atcccctgtg tggtcttctg ctgttgtcac caccacgcgg tttggaccgt cgtaatgggc
    56461 agatgcttca gtgatgtgtg tggggtcaag tttggtgcca ttgacaagtt cgttgcccgg
    56521 ggcggtgaat gcaaacggca gcgcatcggc aaaataccat aaaccaacac catcaaaatc
    56581 cttgagcaag gttgcgcctg gcgggttttc aagcgtcggc gagaattgat atttgggagt
    56641 ggcaacaaaa ggcgcttcgg gcaggttctg actatcccgg tttaataagt gttggttgta
    56701 gttaaagttg atcactggaa gttggacaat gtatgccgcg ggagtccaac tccagtacaa
    56761 ggcgccgccg ccgaggttga tgtagtacat gccttcatct ttgttcttca ggtaacgcag
    56821 agcctgcaga gatttgggct cgatggggaa tgaaacgaaa ccaaagctgt gattggttag
    56881 ataaacatct ttcaacgaaa agaacaggat gacgataaag atgatgatgg tgaggccgcg
    56941 caagcgagtg gttttttcaa gtccatcatt ctggtaggag aaggatatcc tgtaattttt
    57001 gaattgctta cgcaacacga tcatcaatcc ctgcaagctg gctgcactga gaatgagcag
    57061 cggactagcc gccaatatca gcaggcggtt gggaaagcgc agccgataca gaaacgggac
    57121 gtaatcgtat aaatatttga ctgggggata gcgattggcc acccataaca acaagacaat
    57181 ggaaattgcc agcattgccg caagcatgga cctttttcta ctgtgctgga atcccaacgg
    57241 gagaaacaac agcgcaccca gcatcagcgg ccctgtgtaa aaccagttcc agccaccggc
    57301 agtccccaat atttcggtgt tgaaccactc accttgtgaa atgacatagt tcatcactgc
    57361 gtacgaaaca ggctgtgagc cctgctgtac aaggtcatct cctgattccc gtacgatgag
    57421 gcgataccca tcgtaaattg gtagagccat gactgcgatc aaccctgcac taaacacagc
    57481 gatggacaga gcgcgtttga tctggatgaa acgttcttct ggttgggcgg cgatcagtgc
    57541 aacgatcagc atcgtacctg cactggcgat caagtaaaag ggataatagc cgccgccagt
    57601 gaacaagatc atggcaacgc aaaaggctgt ccatgccatg gaggtgatat ctcgtttttt
    57661 gagggcctgc cagaaggctg caaaggtcca tgggaaccag accataccga ccaaaagctc
    57721 ataccagcca agacgccata aaaatgcaag gccgccggaa aacatgaagg tcaaactgct
    57781 ccataagcgg aatatgcctt tgagtccgct gacttgggcg aggtaccact gtcccagccc
    57841 ggccaggaca aaggaaataa agatcgagac cttcatccca ttgataccgc cccataaaat
    57901 gacagggatg gttgcgattg gcgaccaaaa gtgccccagc aagtctccgg cgtgtggaaa
    57961 acctgtttgc atgtagtggt tccacaaggg gatttcaccg tgttgcaata acccgatttc
    58021 ggccagtaat gggcgggtgg aactctcgtt gtgttcaccg gtctgttgta gttgattggg
    58081 gtcaaaatcc agaagtacag gtgctgtaaa aaaatacgca gcacagacaa taatggacca
    58141 ttcaatgagg acggtgtacc atgtggttgg cgaggaaaga agccttcccc aagccctggt
    58201 tatggatttg gtaagattac ttaacgtgtt cattaaaaag attctctctc aggggaagta
    58261 gtaacagggc tgggttgttc cttctgccaa aaccaatagg ataacccaag gccgatcaag
    58321 gtaaggacga aaccgcccaa tacatcggtt ggccaatatc tgaatttgta ggtatggttt
    58381 cctgcggggg ctgaaacctt gagccattga ccttcctcta atggggttga ttttccgtcc
    58441 cgccacacgc gccagccttg caaagcgttt tcgtaaacga ccagatcccc ggtttctgcg
    58501 ttattgcatt taacttgaat atgcccgcca tcggcaatgg cttcgcatgg aattcgacct
    58561 gtatcggttt ctacaaaggc gtaaaagttt tcggggtaaa gcacaaagga aataccctca
    58621 actgttttta gataattggg gtcgttgacc atggcggggt cattgcctga atccagactc
    58681 atgggtgggt ttggacggcc gatcatttcc cagatgcgga aggtttctgt gatcttcaca
    58741 ttatgacgga cggctgctgc cacgtaagtc caatttccat atacggggcg cacccattgt
    58801 gctggttgtg tagggatgga ttctgcggct ctgaccagtt cttttggcca ttcatgcaag
    58861 acacgaaact ggctattaaa ttcgtaaacc gatgtgattg accataatag aatgggggca
    58921 agcaaccagc ggaaatcaat ggggtgactc ttcttcgtga cattattgta gactgaaaca
    58981 acgggccact tcaagttaag aatctgatcg aggccgattg cggccagtcc aaaaatgagg
    59041 gtaacggcca gcccgacccc aaagttgggg ttgcggatat cgcgcagata ccccggcagc
    59101 cagtctttcg cccaacgcaa aggggcgccg cttccgagga aaaagatcaa cgcaattgcg
    59161 attgcgaaga ataaatatag acggaaattt tcacgtgggg tgaagcgcca ggccgcgatc
    59221 gccaataaga tggggatcca gccaatgtac atgacgtata gattgggggc ggcgatcttt
    59281 ccgagcgttt ctgtcatgaa gaaattcata tcgcgaacga ccagattcat tggcatgtat
    59341 tccaatggct ggtctgaacg cggctcggtg gggcgtgaga tttgagagcc aaacgtgaac
    59401 cagggcagca acaccacact ggagaccaat atggcaatga ccagagccag ggcaagttcc
    59461 ttccatattt tgccgatttg cagatatctg ttgattccga aaatcagcag ggctggcacc
    59521 acaccggcaa acagcgccag ttgtaaatag ccttgtccgg atagaatggc ttgagcaacc
    59581 atcaatccaa atagaatggc atcgcgccgc ctgccgtgta acccaagcgc aactgctggt
    59641 gcaaccgtca aactggcggc tgctgttgag aagatgatgc ccacgtcgcc gctaaaaagc
    59701 ggtcccgcca gatggccccc gaccacagcc aatgctgaaa cccatagacg ggttaccatt
    59761 ttcagatcca gcacctttgc cagccaccac agactccatc cgcccataat gagcgaggcc
    59821 agcatggcaa ctttgattcc attggtgacc ccgaaaacca gcatgatacc ggccataacc
    59881 gggtgtaaca atgcaccaat gctatcgcca aatgtgggtg acccgccgtt cagcaggcca
    59941 ttccagaaaa agcaatctcc gcattgcagc acacggatga acggaaagta aggatagatg
    60001 gaaagcagga aatcgtcgtt gccatagccg ggtaccatgt tgcggtcgaa gtttaggtaa
    60061 acgcgaccaa cccaaagtgc ccagagtgcg atcaggggca gttcaaaatt aaatttggag
    60121 atccattgga gtatgggttt ataaaaactt gaaagttgaa tgtcccattt ttgaacgggc
    60181 tttttctcaa cgttctcttt ttttcttttc ttcagccatt tcatcaaaac ccaatccagg
    60241 tagatcgtaa tgatgataac ggcgatcagt gatatttgcc cggaagacat caatgccact
    60301 cctatcgcag gcctgtttcc atgcgtgaaa tgacgagccg ttgaatttcg cttgtgcctt
    60361 cgtagatttc ggtgattttt gcgtcgcgga aataacgctc cacgggtagt tccttgctgt
    60421 accccatgcc gccgtggatc tgaattgcct gatgggtgac gaacatggct gtttctgagg
    60481 cgaacaattt cgccatggat gcttcaagcg aataccgccc gtgattggtt tttgaatttt
    60541 ccttggcaat ggccgcatta tagatcagca ggcgggatgc ttcgatacgg gtcttcatat
    60601 ctgcgatttt gaatcctgtt ccctgaaagg ctgcaatagg ctgaccaaac gcttcgcgct
    60661 gacccgaata ctgcctcgct gcttcgtagg cagattcagc aatcccaagg gcctgagtcg
    60721 caattccaat gcgccccgcg tccaaaacgg tcatggcgat cttgaagcct tcgccctcct
    60781 cgccgagtct gttttcagcg gggatcctgc aattttcgaa gatcagctca ctggtcgcag
    60841 aagcgcggat tccaagtttg ggttcctttt tgccgcgagt cagcccgggt gtgtttccat
    60901 ccaccaggaa agctgtcacg cctttctgat ttttatcagg gtcggtcatc atgaaaacga
    60961 cgaaataatt agccacaggg ccgcttgtca cccaggattt gcgtccattc aaaatgtaaa
    61021 aatcgccgtc gcgtgtggcg cggcttttca tggcgcccgc atctgacccg ctttggggtt
    61081 cggtaaggga gtaggcgcca atggccttgc ccgttgcaat gggggtgatg aaatttttct
    61141 tttgttcctc ggtgccgaat ttgagaatgc catgacagta cagcgaatta ttcaccgaca
    61201 tgatgacccc atgtgaggca tccactttgc agatttcctc aagagcaagg acgtatgcca
    61261 aggcgtccat gccggcgccg ccgtattcct cagggatttc aatgcccatg aaacccagtt
    61321 cgcccatttt tctgatggtg gcgtacggga attctccact ctcatcaaat tctgccgcaa
    61381 tgggcgcgat ctctttttga gcaaaatcgc gggctgcatc gcggagcatt ttgtgttcat
    61441 cgctgagagg gtgaatcggc aattctgtca ttggtttgtc tccaaaaaat cgtttttttc
    61501 gattataccc tttgtcttat tcggaaataa atagttgacg tttattcttg cctgtacata
    61561 taattctgca ctcaaaagct ggcgccctgc cagcggttca gtgcgacaat agataacata
    61621 tgaaaggagg ctgactcgca actaatctgc ttttgtcatg tttccaattg tcgtcataac
    61681 aacttataag gagagagaga atgaaaaagc ttgtaaataa gattttggtt ttgttcgtgt
    61741 ttgccagcct tgtgctttcc gcctgtggcg gaaccgccgc cactgaggcc cctgctgttg
    61801 aggctacaga agcccccgca gaagccactg aagcacctgc tgcaaccgaa gcccctgccg
    61861 ccaccgaggc tcctgctgcc
//
LOCUS       DAOQJC010000005        60424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_188_length_60424_cov_7.950952, whole genome shotgun sequence.
ACCESSION   DAOQJC010000005 DAOQJC010000000
VERSION     DAOQJC010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 60424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60424
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188_length_60424_cov_7.950952"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1186)
                     /gene="neuC"
                     /locus_tag="PLE39_01430"
     CDS             complement(5..1186)
                     /gene="neuC"
                     /locus_tag="PLE39_01430"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNE67012.1"
                     /translation="MTKRKIAVFTGNRAEYGLQYPILRAIAADPRLDYFLLAGGAHLQ
                     QDFGRTMAEIEADGFRVYREVEIRMEHDTLFSTAQAIGTGIVSLSKILDELRPDFLVV
                     YADRYEGFSAMITGTQMNIPTAHIEGGDYTEGGALDDSVRHAMTKLAHLHFATNEQAV
                     ERICKLGEEPWRVFNVGQPSLDLITAGLFASPEEVAAELKLDLSKPVILFCQHSVTTE
                     FEQAAEQVKPSLRALQKLASEGNQIIITYPNNDAGGRRIIDEIEKLKGTPNMHITKNL
                     GRHRFHGVLNIIGRVGRGAFVGNSSAIIKETPAFGCPAVNIGSRQQGRLRADNVLDTS
                     YNEENILAAIQRSIHDEGFRHQCQTCKNPYGAGNAGPKIAEALATIPLDSRLLQKKMT
                     Y"
     gene            complement(1191..2111)
                     /locus_tag="PLE39_01435"
     CDS             complement(1191..2111)
                     /locus_tag="PLE39_01435"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE67013.1"
                     /translation="MEIKNALITGVGGFLGRALAKQLLQEGATVSGLDMQAAPKFLPE
                     GVKYQQADICDFNNLSGIISKTTQKEMVVFHLAGQAHVARSRLDPMSAVTSNVTGTAN
                     LLEACRRIGIQRIVFPSTVLVYAKTPNLPFTESDAVQANSIYAATKLAAEDLLAGYAA
                     EHGFSCRIARLGNVYGPGGSTDSVIGIILQQVRSGGPISIKTLSPIRDFVYRDDVVHG
                     LIALGKDASESGCEVFNLSSGIPTSIRELAETASLLGKQKISVTETEPPSSGVEDRIL
                     LSVQKLKTHTQWQPQWSLEAGLRQTLLEMG"
     gene            complement(2115..3143)
                     /locus_tag="PLE39_01440"
     CDS             complement(2115..3143)
                     /locus_tag="PLE39_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018401706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNE67014.1"
                     /translation="MSFKIGNRMIGMEHPPLVIAEIGINHEGSYEKAIRMIDDAHAAG
                     CECVKFQSHVVDDEMIPNDVVPGNTTETIWDIMVRCALSEEEEIKIKDYVESLGMLYL
                     CTPFSRAAAERLKKMGVSGYKIGSGECNNYPLIKHIAADGKPIILSTGMNDLESIAPA
                     VDILRQAGVPFALTHCTSMYPTPYEKVRLGGLADLAKKFPDAVVGLSDHSLGNYTCFA
                     AVALGARILEKHFTSDKTWPGPDVPISIDPIELKELVEGSRAIHAALGGSKTILAEEK
                     PTIDFAYACVVSIKDIQPGEKLSAENIWVKRPGTGDIKAVHFENLLGKTAKKTIMKNS
                     QLSWDDFE"
     gene            complement(3166..4269)
                     /locus_tag="PLE39_01445"
     CDS             complement(3166..4269)
                     /locus_tag="PLE39_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNE67015.1"
                     /translation="MTYPNISNLFVKLGTSIREVIECIDRSGRISIALIVDDEQRMLG
                     TITDGDVRRGILAGISLNEPVSKLLPIKAQMPHPIPVTAPVDTDPSQFLSIMQEHAVK
                     QIPLLDKDGRVVDIVILSEILSQPKLPLQAVVMAGGLGTRLRPLTDNIPKPMLPVGGR
                     PLMELTIEKLQKAGIEHVDVSTNYRASNIVEHFGDGKAFGIELNYLQEDRPLGTAGAL
                     GLMDTPEKTLLVINGDIITHVDLEVMLSYHKKQHAELTVGVRQYGVQVPYGVIDCDGP
                     NIVSLREKPQISFLVNAGIYLLEPSVYNLIAKGERMDMTDLIQRLIDAKRNVVSFPII
                     EYWLDIGQPADYERAQNDAKDGKYNHNSKSTPT"
     gene            complement(4285..5088)
                     /locus_tag="PLE39_01450"
     CDS             complement(4285..5088)
                     /locus_tag="PLE39_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE67016.1"
                     /translation="MKSLKELMNLDGRTALITGGAGHIGSAIGEALAELGANIVVLDS
                     SPSVCAEVANKIQQQFGPQTMPLSIDLADENSVRSAPEKVLTKFGRLDILVNCAAMVN
                     TSNLKGWVAPFQEQGAEAWRLALEVNLTAPFILTQVFADALKASGHGSVINVSSIYGM
                     VGPDMRLYDETPLGNAAAYGASKGGLLQLTRWLATVMAPHVRVNAISPGGVWRNQPEL
                     FVKRYEERTPLKRMGMEEDFKGAAAYLASDLSAYVTGQNLAVDGGWTAW"
     gene            complement(5158..6960)
                     /locus_tag="PLE39_01455"
     CDS             complement(5158..6960)
                     /locus_tag="PLE39_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNE67017.1"
                     /translation="MIKVSDYIVKFLVEKGIRDIFLVSGGGIMHMLDAVGRNPDMNYY
                     CNYHEQACAISAEGYARVRNDVGACLVTVGPGGSNAISGMVGAWYDSIPMIVICGQVR
                     RNIIADYSKVRQIGPQEGNNLAMVKPVTKYANVVLDANMIKYELELAYHQAISGRPGP
                     VWLDIPLDIQDSQVEESQLPSYHPKVDEKLSQGTQLTSDVKNALEMIKNAKRPLLLCG
                     NGIHLSKSDELLKRLIEKLNIPVVLPDAAKDLVAEEHQLYMGIFGTAGQRRANFAVQN
                     SDCILSLGAGLCIKKVGFNYKGFAPNAKKIIVDIDEGQLFHQVLTPDLPIQTDVRFFM
                     EEMLGQLQTVKVEPSAKWLEACLRWKERYPLIVDEYYTDKDFVNSYVFIDKLSDLLSN
                     TDALVAGNGLDTVSYLQVFKVKEGQRTYTSINWGAMGWDLPLAVGTCIGNDKKRTICV
                     TGDGSLQWNVQELLTIKNYNLPVKIFLFNNRGYSSIRATQKTFYNGRFVGSDESSGVS
                     NPNFEYLAKAYGLEYVSIKNNDGIEQGIQETLTLNGPVLCEVNISPEQGITPKASSFV
                     REDGTIESRPLEDMFPFLPREEIWENMHLFDNDK"
     gene            complement(6980..8017)
                     /locus_tag="PLE39_01460"
     CDS             complement(6980..8017)
                     /locus_tag="PLE39_01460"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE67018.1"
                     /translation="MMQNLPSTDLGHILDHTRNLWEDLRGARLFITGGTGFFGTWLLE
                     SFLFANRQLDLKASASVLSRKPASFQKAFPHLGLDPAITLIKGNARDFKFPRGNFSHI
                     IHAATETNPDFTDVDPLEKFSSNIEGTRHVLDFAVKSGAEKFLFTSSGAVYGKQPAEI
                     THIPETYLGAPEPTDTNSAYGQSKRASEFLCAAYAKQHGFDAAIARCFAFVGPRLPLN
                     SNYAIGNFIRDAMSSTSIQIGGDGTPYRSYLYAADLAIWLWTILLRGQSCHPYNVGSE
                     NDLIILQLAKTVADVIAPELPIQVAKRPTPNKTAERYVPSTQRAKTELGLQEFTKLPD
                     AIQRTAQWYAL"
     gene            complement(7992..9311)
                     /gene="rfbH"
                     /locus_tag="PLE39_01465"
     CDS             complement(7992..9311)
                     /gene="rfbH"
                     /locus_tag="PLE39_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HNE67019.1"
                     /translation="MSERSEQLRAKILELTVEYAAEEFPERKFVPGVSSVPVAGRVFD
                     SADVQSLVDSSLDFWLTEGRFARQFEKDFARFFNMRHSILVNSGSSANLLALTCLTSP
                     LLKERQLKPGDEVITVATGFPTTVNPIFQNNLVPVFVDVDIPTYNIDVNQLEAALSQK
                     TRAVMIAHTLGNPFNLEVVTAFCKQHNLWLVEDCCDAVGATYANRMVATFGDVATVSF
                     YPAHHITMGEGGALLTDKPKLKKIIESFRDWGRDCWCEPGKDNTCGKRFDWQLGDLPQ
                     GYDHKYTYSHIGYNLKATDMQAAVGVSQIKKLNGFIEKRRENFNYLKEQLSPLQDHFV
                     LPEATQNSNPSWFGFPIFVRENKNFTRDAILRSLEARKIGTRLLFGGNLIRQPAYRNQ
                     NYRIIGDLKKSDEVMHNVFWIGVYPGLTRPMLDYVVEALHDAKSSLH"
     gene            complement(9304..10299)
                     /locus_tag="PLE39_01470"
     CDS             complement(9304..10299)
                     /locus_tag="PLE39_01470"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNE67020.1"
                     /translation="METMELNKAFWLDRPTFVTGGTGLVGTWLVKRLLAAGADVVCLV
                     RDWVPQSEMVRAGYIEKVKVVRGDIRDRDMLERTLGEFEIDTVIHLAAQTIVTIANRN
                     PVSTFETNIAGTWNILEAARRSPKVKQVVMASSDKAYGDQEILPYDEKTPLQGQHPYD
                     VSKSAADLIAHTYAKSFDLPVAITRCGNFYGGGDLNWNRIIPGTIRSILRGQRPIIRS
                     DGEYIRDYFYVEDGAAAYMLLAEQLATRKELKGEAFNFSNEIQVSVREIVDHVLELMN
                     SDLKPEILNETSNEIRHQYLSAEKARRVLNWKPLFNLDDALALTINWYKDFFKHE"
     gene            complement(10281..11051)
                     /gene="rfbF"
                     /locus_tag="PLE39_01475"
     CDS             complement(10281..11051)
                     /gene="rfbF"
                     /locus_tag="PLE39_01475"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HNE67021.1"
                     /translation="MKVAILAGGLGTRLSEETTVKPKPMVEVGGKPILWHIMNIYAAY
                     GFKEFVVALGYKGEIIKDYFLNYHYRSRSITVQLKTGGVTAHNIEGEDWTVHLLDTGA
                     DTNTGGRVKRVAEFIGNETFMLTYGDGVSNVNIPNLIDFHKQQKRLVTMTAVRPPARF
                     GQMVIEDHRVVQFKEKPQIGEGWINGGFFVLEPGITQYIQGDHTAWEFESLEHIAADK
                     QLSAYQHEDFWQCMDTVRDVHNLEKLWQEGNAPWKLWN"
     gene            complement(11199..11272)
                     /locus_tag="PLE39_01480"
     tRNA            complement(11199..11272)
                     /locus_tag="PLE39_01480"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11236..11238),aa:Val,seq:cac)
     gene            complement(11318..12520)
                     /locus_tag="PLE39_01485"
     CDS             complement(11318..12520)
                     /locus_tag="PLE39_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNE67022.1"
                     /translation="MQNDFNEDSTSSAKPKEAKQAEPVRQRSFFTRWMNTLVQMGLGE
                     SLLRSGTNLFSIAAIVVVILLVQAFNTQRANPASGNSDQASGPAPTVEVDIHSIPALE
                     NPLITGISRSAQIHTNIPSRPRNEITNYTVQEGDTVFGIAEKFGLQPQTILWGNYNIL
                     LDDPHSLKPGQELNILPVDGVYWEWLGGIPFGDWAEFYGVTAADIIEYPSNKIDPNAV
                     GDYANANIKTGTWLIIPNGKREFVNWSAPLGVTRENPASARVLGQGACEPVSGGAVGY
                     GTYVYPTNKHFLSGFDYNEKTNHRGIDLAGSEGEGVYAADAGVVVYSGWNNYGYGNMI
                     MIDHGNGFQSLYAHLSAIYRGCGQSVGQGEAIGAVGSTGRSSGPHLHFELMTATWKVN
                     PWDYLPPP"
     gene            complement(12603..13274)
                     /locus_tag="PLE39_01490"
     CDS             complement(12603..13274)
                     /locus_tag="PLE39_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNE67023.1"
                     /translation="MCGRFTLTVDPAELQDTFSEFTFPTQFSPRFNIAPTQPVLAIPN
                     DAKNKADFFLWGLIPSWAKDPSIANKLINARGETIAEKPSFRGSFKYKRCLILTDGFY
                     EWKSQPGAKTKTPYFIHMKDRKPFAFAGLWDEWHSPDGGSVRTCTIITTEPNELMSTL
                     HNRMPVILDAQNYADWLDAAPRTPDSLLHLIRSFPSDKMSAYPVSTMVNAPGNDRPEL
                     VVPVQ"
     gene            complement(13290..16427)
                     /locus_tag="PLE39_01495"
     CDS             complement(13290..16427)
                     /locus_tag="PLE39_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE67024.1"
                     /translation="MGNSFKPPTFDSEEKSHQAFLLRAIVWGMILVPIPYLIFIAITN
                     PSGFNRVLAQSAFGEAINLLLLFMLRKGIVRLAAILQIVMLWIFFSITAVSGEGVQSE
                     AYLMGFPLVILIAGVLFGGRAASYVTVASLLTGLVLIYAETQQAILSTNLTRSMNMWV
                     ISLVIFPIITTLQYLSSLSVQNALKRARISERKYMLLSNISTDYVFETKVDEAGNNTL
                     FWVGGAFEKMTGYTLEEYLAAGSWSAHVHPDDIEKDIRDMEALARNEDIKSEIRTINK
                     NGETHWERIYAHPIWDDYNNRLAGIIGAVQDITESKHAEETLIATLSQTAEMVRKIPD
                     MAWLKDVNSKYVAVNEKFASVAGRPMEEIIGKSDDEIWNPEFAKNYREDDLLVIETLK
                     LRRVEEKQMDRLGREYWVETIKTPIFNGKGEVIGTTGVARDINDRKEAELAEKRRREM
                     LEKVIELGKLVTEASDIKGTIKKIWHGVHDDLQFDRLAIFLYNAERNQMDDTLGTDIN
                     GQMTDNYGLSFPVGEDTDGSTFAYLLEKPDGFYFTKDYAKENNIPPGHEMYHVKEYTA
                     VAVWAGNKPVAVICADNLITLRPITNEQLEGLRLFAGYAGLAIENSRLHSAIQNELDL
                     QKSAEQREARRRATLEKVIQLGQSMTEVHNLRATLERIWHGVHDELAFDRLGIYMYNP
                     VRKSMDGTFGTNDRGEMVDEWETWKSIDQGNEEANSFLYLLNNPDSIYLTRDYESEHK
                     SAINDIMLGVKDFAAVAAWVGEKPVAVICVDHKISGRPISDEQLEALRLFAGYAGLAI
                     ENARLNTALQNELTQRKTFIEELEAKNAELERFTYTVSHDLKSPLVTIKGFLGYLEQD
                     ALSGNFDKFKQDVSRIETAVGKMQNLLRDLLELSRIGRLINTPADIQFDEIVKDALEI
                     VHGQIEGKSIHIIHKQTGIMIHCDRVRLTEVLQNLIENAIKFMDNQPSPRIEIGAFEN
                     ERGQATFYVKDNGAGIAPEFHEKIFGLFNKLSTESSGTGIGLALVKRIIEVHGGRIWV
                     DSQINRGATFYFTLS"
     gene            complement(16450..18345)
                     /gene="selB"
                     /locus_tag="PLE39_01500"
     CDS             complement(16450..18345)
                     /gene="selB"
                     /locus_tag="PLE39_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HNE67025.1"
                     /translation="MRVIGTAGHVDHGKSTLIAALTGTHPDRLKEEQAREMTIELGFG
                     WMNLPNGEEVGIVDVPGHRDFIENMLSGIGGIDAALLVIAADEGVMPQTKEHLAILDL
                     LQIPAGLIVLTKTDLVSDSTWLDLVETDIRGAVSGTVLQDAPILHVSAKNKLGLKELT
                     ASLQTLLENKPARPDLNRPRLPIDRVFTMSGFGTVVTGTLSDGHLTVGDEVEILPSGQ
                     KGRVRGLQTHKKKEEQALPGSRTAVNISGVNTEQIQRGEVVSHPNQYTPTRRIDARLR
                     LLKDISKSIKHNDEVKFFTGASETIATLRLLGAEELRESEEGWIQLELRDPIVAVRGD
                     RYILRRPSPSETLGGGVIVDHQPKGRHKRFDETVLKSLESLAQGSPADILFEAALALN
                     TASVKEMVARSRLESAPAGEALQELLADGRLVLLEEGEATVASDLLAIAIPHWSILQE
                     KTLQLVKTYHQQFPLRRGIPREELKSRLKLTPRVFNATVNFLTHHSALTDLRGSVAQT
                     THEIKFNGQDQARVNGLMRKFEANPFGPPSVKECQSEVGEEAFNALLEMNELVSVSPD
                     VVFKKQDHELIVIKIRAELKNKGTITLAEVRDLLGTTRKYAQAILEYMDAINITIRDG
                     DARKLRN"
     gene            complement(18411..19598)
                     /locus_tag="PLE39_01505"
     CDS             complement(18411..19598)
                     /locus_tag="PLE39_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67026.1"
                     /translation="MEFEQIIKRLEWLDKQQRKNQDALTSLDGRLESFETTINAVSKQ
                     MKGFTKQITDVSPMEKRLDQFETLMTRQRAEFVKLLEENEKAQTRTTKENLKYVQTEL
                     TEIQKVIANIKTTTNLTEIKKQLKERADEMQRVLNNVADLKFRVDEATRTKDDAVHAL
                     KAIEETRKNDLKRVADIQGEVTALRKRVDENRDKATITADSIRNVENRFTDLIASELE
                     RKQAQAAFLEQQTLAQIDRDRAWKEWKEKYESFQKEAANLDIHIQNLDEALRGAKKAQ
                     ETYLELNTKLERRINEVTEMQRLAEDRLRQEWVSFKTDDQKRWTGYSLSSEESFRDIR
                     KEVQKLETRITPFDDITQVLQDQMHQTTDATEKQLQEMMNVLHEWMLSYQRIMGHGKK
                     SKK"
     gene            complement(19660..21057)
                     /locus_tag="PLE39_01510"
     CDS             complement(19660..21057)
                     /locus_tag="PLE39_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNE67027.1"
                     /translation="MSAPIIWILIPFVAGVLILFLLQERISSFVGGVVSMLLALTALI
                     FPIDTALLIGSVSIKISSTVQFFGRSFALNTADGPLLAIIYGLATLWFFGTQASGTAN
                     RFVSLGLMIVALLTASIAVEPFLFAALLIDMAAMLVIPLLVPLHQRPGQGIVRFLIQQ
                     TLAMPFVLFSGWMLAGVEASPGDIGNTIQAGTMLSLGFAFLLAIFPLYSWIPMLTEEA
                     SPYATGFLLWALPTFTIIFLLNFLDRYTWLRSTPQLSNAIQFAGVLMVASGGLFASQQ
                     KHIGRMMGYASIVETGLLVLAAGLKSTEVVNVTFLLLTPRGLELAVWALALSIIKRKV
                     YSLRFSEVRGMARQYPIAVAAIVLAHLSMAGFPLLAGFPSRAALWQELTPESLTMSFW
                     VLIGLLGLLMGAMRTLAVFVMAEENRTWEINESWVQMGMLGVGVIGLFILGIFPQTLQ
                     PFIASLPALFEHLGQ"
     gene            complement(21054..21665)
                     /locus_tag="PLE39_01515"
     CDS             complement(21054..21665)
                     /locus_tag="PLE39_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67028.1"
                     /translation="MATDIISWIAVGMILATSAIILISRDWRIPLGALALQYLAAFWL
                     VTRHLPFVMGSAKLITGWMVVAAIGMTRLGLTATEEKAEDTSLQSGRWFRGILIGIVA
                     LVTAGATPRIETSIPGLGLPVIAGSILLIGAGVIHLGVTSDILRVTLGLLTMLTGFEV
                     LYAAVESSILVAGLLALTNLGLGIAGSYLLIAGSIPLEVEDEE"
     gene            complement(21667..23220)
                     /locus_tag="PLE39_01520"
     CDS             complement(21667..23220)
                     /locus_tag="PLE39_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67029.1"
                     /translation="MLILISCLLLFLTALILLVLGVTQPNARYTWLVAIGGAMLSLAS
                     VFIWLARIPVDFTLPAWQPLTLFQNPILFRADAISWPYAVAISGITLTILLTEVTRPN
                     STSLYAWAGVLALGGLGILAVTANNPFTLLLIWSALDLAELITQLRSVDGPSNNERVV
                     ISFSTRAFGTFLLLWANVISIAQGSTFDFHSIAADSGLYLVAAAGLRLGVFPLHLPYS
                     SESVLRRGFGTALRLVAAASSLILLAHVPAGSLNSSVTPFLLLFALIAAVYGGWMWLR
                     APDELTGRPYWIIGIAALSITATLNGNPLGAIAWGCALILVGGALFLSSVQHKWLSRF
                     LLVGAWSLSSLPFSLTASVWLGNLNFFVPFGVAAQALLTAGLIRHSLRPGDRGSLDSQ
                     ENWVNRIYPAGIILPAVLQIILGLIGWDGARQIGAWLLAVIASLLILGLVWATPRFRI
                     LNPIRAHWVNPIAANTPSVFYSSFWAVYRVFERINLTITTALEGNGGVMWTLLFLVLF
                     ISLLTQGLP"
     gene            23291..24559
                     /locus_tag="PLE39_01525"
     CDS             23291..24559
                     /locus_tag="PLE39_01525"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HNE67030.1"
                     /translation="MILQRRILALLAFFLATSCASTPDPLPASVPLAAPTLTPFQPLP
                     EATSTPDVAAIPAEPTYTPLPRQNTAIEILPTPLLIDPSANGSAGDFSLMNINNPLTG
                     LPVNDPALLERRPMVIKVANAPDTIRPQSGLTLADVVYEYYIEWGDTRFIAVMYGNDS
                     PKVGPVRSGRYFDEHVARMYNAFLVFKFADPREYSYLKNSTLNEFLVVPGGSGCPPFI
                     FGPDKKRDDYNNFFFVTSEWAACAASKGLDNSPQVIRGGFFSDAPMEGTTTASRIFSY
                     YSRQSYNYWEYDVITHKYFRYQEANDMLNGKPEAYAPLSDAETGLPVNAENIVVIFVP
                     HTFANEFNAKDEVYNIDLIDYGNAFVFRDGFVVPAKWERTDINQPLLLTNLDGSPIYL
                     RPGRTFYEVMGFTSTYTQDGSDWHFKFETP"
     gene            24627..25157
                     /locus_tag="PLE39_01530"
     CDS             24627..25157
                     /locus_tag="PLE39_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE67031.1"
                     /translation="MTFDPVFLGSLTLVLTAFGGAFLAALWISLVVWTYRDIRARARD
                     PLTQTLAALLVAVLNLPGVLVYLILRPPRTLEEEYQRTLEEEALLQALEDLPLCPGCE
                     RRVKEDWQVCPNCHTKLKKSCHNCGKFMELPWNICPFCGTPAPGMRLESTGMDDALRN
                     LKLTDDSTEVKVDSAG"
     gene            25348..26463
                     /gene="menC"
                     /locus_tag="PLE39_01535"
     CDS             25348..26463
                     /gene="menC"
                     /locus_tag="PLE39_01535"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HNE67032.1"
                     /translation="MKIDSAVLHHLSMPLVAPFETSFGRETDRECVLIELRSEGLVGY
                     GECVASRDPGYNYETTGTAIHILKDFILPLILGVDVKDALDFQTRVAGIRGHHLAKAG
                     VEMAIWDLLGKRSGKSLREMLGGAREKVEVGVSIGIQESAQGLVKTAAEFIEQGYARV
                     KIKIKPGRDVEDAAAVRKAFPDLRLQVDANSAYSMDDAPLLKPLDKMNLLLIEQPLFE
                     DDIWDHHKFQEQFETPVCLDESIVSPRHARYAIEMKACRVINIKAGRLGGISQGVMVH
                     DMCMEKSLPVWCGGMLETGIGRASNLALASLPNFVLPGDVSASDRYYKRDITNERFVL
                     NPDSTITVPNGPGLGMTIDEEALKLFSLATLSIVANS"
     gene            26532..28343
                     /locus_tag="PLE39_01540"
     CDS             26532..28343
                     /locus_tag="PLE39_01540"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNE67033.1"
                     /translation="MPRIIFLERLESYLAGRFTLIKSSFFVRQPNWNGHRATRLGFLL
                     ISILSLVACQPSLWGAPPPPPTFAAATPSAMPRIPTLAPTITPMPPTAVPTPVVLNAA
                     QVWASPATPLALREKLQHWGFQVLTTNQASANLQIDVAQPITGAFDTSTWTYALVAPF
                     PTIIDGLSSQELISVWSGASFGPFAGIPILMEESTMMTFTALWGAPAAGSVQVVPADQ
                     LLDTAWNQMPSWAIIPFEKIEPKWKVLTIDGQSPIQKHFDPVIYPLTVTYRLTCAEPC
                     EVPAELDFSYQNRDPNKLATVIMTGVTAMVRATAKTMDVKGITYPGEEIRDLLREADV
                     THINNEVPFFTGCPSPDPNQEKQIFCSSPRYMELLTDIGTDVIELSGDHFADYEDVAM
                     YETLDIYKQYNLPYYGGGYNVEDGRKPLLMEVNGNKIMFIGCNYKTIYASATDTVPGA
                     VPCDFDYMTEQIAYYRSQGYLPISTFQYHEFDTAKARPQQIIDFRRMADAGAIIVSGS
                     QAHVPQEMEFYNGGFIHYGLGNLFFDQIAKRGPRLTEREFIDRHIFYDGRYLGVELIT
                     AWLTDYARPRYMTELERTRFLTDIFKESEWGFLQVGQ"
     gene            28425..30098
                     /locus_tag="PLE39_01545"
     CDS             28425..30098
                     /locus_tag="PLE39_01545"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNE67034.1"
                     /translation="MRKIPVYLLCLAMLAACVPATNAPAVATNPIPTATASPQPVSTN
                     TPMPAALWIAPAVPAVLHEAARSSGLPITEDPALATQKLDMSDSGSLWIYALVAPFPT
                     LTDDVASQDLLSTWKGASSGPLNGHSVLLAESTLRAFASVWGEPASGSVRSVPAGEIL
                     DTAWKESAWAIIPFEEIQPKWKVLSVDGQSPLRKSFDAEVYPLKIHFSLSQPEEFALP
                     ETNRDPAKLTTVILTGVTALVRATAVTMELRGVTFPGEFVRDELREADITHISNEVTF
                     DKDCPTPSSGYRNFILCSDPKYMDLLLDVGVDVVEVTGDHLRDRGEVALLDTLAMYKA
                     NNLPYYGGGINAEEARQPVLMEVNGNKIAFMGCNGKREGKYPKASATSPGPALCDFDF
                     FGDQMQALKAQGYNIIFTFQHEECYSPGPCYTHEEGFRRAADEGAVIVSGSQAHFPHV
                     MEFHGDSFIHYGLGNMFFDQMTYNLPSGEVIDETRREFHDRHVFYDGRYLGVELLTSI
                     LEDYARPRPMTAAERLQFLSDYFYYSGWMPLLPTPVPQPTVTLTPIALP"
     gene            30222..>30835
                     /locus_tag="PLE39_01550"
     CDS             30222..>30835
                     /locus_tag="PLE39_01550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE67035.1"
                     /translation="MQKIFALLEKRSPYFWIFTSLVIVGLLGVVDYLTGNEFSFSLFY
                     LIPVSLTAWYVSSAAGAIFSILGAVTWLIADLVIGENYSSKFVYLWNTLIRFGFFAIV
                     SYLMSELHRAQNKMQTLARTDFVTGAFNSRYFHELLKTELDRSSRYKRPFTLVYLDLD
                     NFKQVNDRFGHDEGDKLLTCIAQELKQAVRKSDIVARLGGDEFAV"
     assembly_gap    30836..30935
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <30936..31225
                     /locus_tag="PLE39_01555"
     CDS             <30936..31225
                     /locus_tag="PLE39_01555"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE67036.1"
                     /translation="AVRKSDIVARLGGDEFAVLFPEAGRQEAEAIMAKVHRHIAEQLK
                     QTHSYVTFSAGAVTFESSPISPAETLRIADKLMYAVKNSTKNGIQFSTYSG"
     gene            complement(31229..31996)
                     /gene="fabL"
                     /locus_tag="PLE39_01560"
     CDS             complement(31229..31996)
                     /gene="fabL"
                     /locus_tag="PLE39_01560"
                     /EC_number="1.3.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabL"
                     /protein_id="HNE67037.1"
                     /translation="MFQNKIALVTGSGRGIGRAIALHFAKHGADIVVNFFRNRTPAEE
                     TAREVEKLGRRALLIKADVGDLDDLNRMFDEIQAGFGGLDILIHNAASGYNRPALEQK
                     PKGWDWTMNINARALLFAAQRAAALMEIRGGGKIVSISSAGATRVLPDYVVVGASKAA
                     LESLTRYLGVELISKGINVNAVSPGVVETDALHHFASMGNQDNILQNFIAQVPAGRLI
                     TPEEVAEVTAFLCTPAAAMIVGQTIVIDGGYTLPIPQ"
     gene            complement(32047..33120)
                     /locus_tag="PLE39_01565"
     CDS             complement(32047..33120)
                     /locus_tag="PLE39_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE67038.1"
                     /translation="MSKKLYLYSLVLLLIACSTQSPAISPPTPVATATQTIPPSQTAS
                     PTFASTFTPIPTATPIPYPMSIIAMRNGEYPGSEITIVKELDRGSNYRRYYSYYLSEG
                     LKIYALLTIPNGEPPEGGWPAIVFNHGYIPPDVYRTTERYIAYVDEIAKAGYIVFRID
                     YRGHDASEGEPTGAYGDPGYQIDVLNAVAAIKQLPEVNPDKIGMWGHSMGGYLTLRAM
                     VISQDVKVGVIWAGVVASYQDLLYNWRRTGSFTPSPSSRGVGWRTRWIEEFGTPEQNP
                     VFWDSISATSYLSDLSGPLELHHGTADEDVPLDFSIRLAEMARNASQTADLYIYEGDN
                     HNISANFSIAMERTIKFFDSYLK"
     gene            complement(33163..34455)
                     /locus_tag="PLE39_01570"
     CDS             complement(33163..34455)
                     /locus_tag="PLE39_01570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE67039.1"
                     /translation="MSFLAVLAVLLLTYQYTKREWEYSQKEMISQAGLLLDTISLTLR
                     DPLYNLEIDELKDLAGKIGENPQITMFVVYDKTGRILVDSSKPELEFSQEVDVLGYNL
                     VTTPTHELYISWQGTQLVSGKSIRIGNQTIGAIAIGFSTEKHDQKVKDLTLQGLTLAL
                     VVIFLGGLFTFGTARQITSPLSELARVANKMKAGDLTVRANQPRVNDEIGQLSTAFNQ
                     MAESIQQRQSELRELANGLEKTVNERTAELQEQAKILEEMAITDPLTQAYNRRQFYKL
                     AKIEMERAQQQDGKPLSVIVIDADHYKRVNDTFGHQIGDLVLIKLVEICHNSIRKTDV
                     FARYGGEEFVILMPETDSETARVIAERIRERVESTKLPVDGQLVQITISLGVSTYSNA
                     YSLSFDSLLTQADRALYKSKRLGRNRVTVWADEMLVPG"
     gene            complement(34613..35485)
                     /locus_tag="PLE39_01575"
     CDS             complement(34613..35485)
                     /locus_tag="PLE39_01575"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNE67040.1"
                     /translation="MHTILKSEKKEVVIGIDKPFVMIGEKINPTGIKKLGQALVDKNM
                     DYVKQLAKRQVEWGADVLDVNVGHPQIDEAAIIPMVVEAVKSVVDVPLCIDSNDPKIL
                     EAGLKVAPGKPLVNSVNGEEKQLETVLPIVKDRGAAVIGLTIGDDGIPPTAELRLAAA
                     AKIIERAAKIGIPIEDIIIDPLVMTVGHDSKAATVTLKTIELLVKEFGVNINLGASNV
                     SFGLPDRHSVNAAFLALAMQTGVTCSITDPIKLGSTIKATDLLLGRDANSMRYLKYFR
                     ATEKLREAEAAAKQ"
     gene            complement(35505..36662)
                     /locus_tag="PLE39_01580"
     CDS             complement(35505..36662)
                     /locus_tag="PLE39_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNE67041.1"
                     /translation="MLKQANAISEELIEWRRDFHMHPETGFDVHRTAGIVADELEKMG
                     YRVQRGVGKTGVVADIGEGGKLVAIRADMDALPIFEQNETDYVSQNTGKMHACGHDSH
                     TAMALGAAQILAKEKFTGRVRFLFQPSEETADEEGKSGAQRMYEEGATKGVDYVIAQH
                     VDPTSPVGTIAIEAGPSGGGVDTWFATINGKGGHGAYPHTTVDPFVLLAHVIMGLNNI
                     SSRRMNPFEPVALSIGSIIGGDAENVIPDQIKLKGTLRFTSLETQKLLRDEMRRVFEI
                     TKTLGGDYELDFLFGGLPMINDGHVSKVIDQVGKDLLGAENVHPMHKSLGAEDFPEFL
                     RDAPGAMYTLGTMIAGREVYELHHPKFDLDERALPIGTAVLVETTLRFLKE"
     gene            complement(36697..38115)
                     /locus_tag="PLE39_01585"
     CDS             complement(36697..38115)
                     /locus_tag="PLE39_01585"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNE67042.1"
                     /translation="MEPLKFLSENDVKAMHEATLRIMSEVGLIWTHKPSLDILLGAGC
                     TIKGNRVCMSPELVMDCIAKANKRPKIRGRNGITNELGGGNLYFHNLGGARDVFDART
                     GTRKIATDQDVIDATRVLDALPNCHTVTPFFTPQDVPGELMSLYMYRHSLPYTTKPLQ
                     GPGIQFAEEVKYAVEMAQVVGTPPEELTLSLSPVSPLTIHDPAAAAIVEMAKAGVICS
                     NLPAPTGGATSPMTITGSLVQQSAETLALVVLAQIVNPGCGVVYCGRLGMLEPRTGLI
                     WGGVELGLSSAGTVQLGHYYGFSVNVYGFSTNAHTLDAQNAFERGLNAAIPALAGADE
                     LSGIGEMEAGVMGSYAQMVLDNELVGSVMRLRSGLSADVEHLAVEIIGNVMDGTRNYL
                     GQKHTLKHLRAGELALTKLAERNSWETWEDKLERKQMADYAMEESERILREHQVPPLE
                     PEQEKELDRIMAAAEKEMVKKK"
     gene            complement(38135..39919)
                     /locus_tag="PLE39_01590"
     CDS             complement(38135..39919)
                     /locus_tag="PLE39_01590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNE67043.1"
                     /translation="MLNLAQLLRVPHVDNVLPYDVSPDGQRLIFSWNKSGKWELWEAG
                     ESGTRELMLGLDGAKFSPRFSPDGSMLAFALDLDGSESYHIAIHDFTTNTTTDLTPSI
                     AYAHQPNVSWSPDGKMLAVLSDMKGQFAAYLLPIDGSPVRLLKNIFHPCWDAVWSPDG
                     QWIAIESESTASDRSIHVVPVGRTRKGPKVNTVQLKVNGRVLNAQHPAWSPDSKFLAF
                     CGENVEWHDIGLFNVETQEITWVNESVGDDTQPAWSRSGDTLRVGWVHSEGAQTSFQF
                     KKGSADVAQIQVGAGVHSFPKFTAEGVVILYEDSSRPCDVWTLNLEDGSCKQLTDSLP
                     AELKDVQFVQPEEVWYAGMDGVQVPALLYRGQSERAVIDIHGGPNWHVQSLWSPTTSY
                     LVSRGWTVLTPNYRGSTGYGKKWQNASRYDMGGVDTDDCAAGVKYLVENGLAEKNKIT
                     VTGRSHGGFLTMSCLTRYPDLFAGGSAVVPFLNWFKSHKDSREDLQHWNIENMGDPED
                     NKELWHSHSPYFFLDKINAPVQMICGGNDPRCPASDSIAARDKLVELGKEVELLLYKD
                     EGHAFLNIENVIDAEEKSAAFLEMVLKK"
     gene            complement(39972..41450)
                     /locus_tag="PLE39_01595"
     CDS             complement(39972..41450)
                     /locus_tag="PLE39_01595"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNE67044.1"
                     /translation="MQPKLSLLDDQLIERIIEEAYQLMLKPGIKVQNPEARQLLADAG
                     AQVDEETMVVRIPEQIVRTALQTVPRQFFLYDYDGNPAVQYGGDAVQFDPGSSGISIL
                     IPETLEHKTAETADLIRVIKVAESLPQYDAQSTAIVCHDVPKDIHDLYRLYLVMMYSK
                     KPIVTGAFTNKTVHQMIEMLAIFAGSHENLRKYPRAVFDACPSPPLIWSNFGAGNLIA
                     LARAGVPAEIVSMPLAGAAAPVTLLGAVTQHAAECLSGITIHQLACAGSPIVWGGAPS
                     TFDMRKGGTPFGSVETAMIDSSYAQVGKTLGLPTHAYLGATESKILDMQAGLESGMGA
                     MIGALSGINMISGSGMVDSLLCQSPEKLVIDAEGIAMAKRMVQGMKIQTETLATGFYE
                     GVNFKGGDFLKQKITMKLFKEEQHMPSKVIDRDSMRGWKESGSQDTFSRARTRVDEIL
                     ASYRRPELDPAKVAELHAYVLNLAQQAGLEQLPVVEAMEVAG"
     gene            complement(41580..43058)
                     /locus_tag="PLE39_01600"
     CDS             complement(41580..43058)
                     /locus_tag="PLE39_01600"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNE67045.1"
                     /translation="MQPKLTLLSDEIIARILEEAYELLRCPGVKVQNPEARDLLASAG
                     AIMYPDNNVVGIPEALVKKALESVPREFYLYDYDGNPTVHYGGDSVHFDPGSSGITML
                     NPETLEHDTTETEHLIRLLKVAEQIPQYDAQSTAVICHDVPKDIQDSYRLYLTLLYSK
                     KPIVTGAFTNKTVQEMIDMLALFAGSKEAAREKPRAIFDVCPAPPLIWSNFGAGNLIA
                     LARAGIPAEIVSVPLAGAAAPVTMLGTVTQHTAECFAGIVIHQLAHAGSPIVWGGAAA
                     IFDMRKGATPMGAVETAMLDCSYVQVVKTFNMPTHTYLGATDSKLVDAQAGLESGISA
                     MVGALSGINMISGSGMLDFLACHSAEKLVMDAEGIAMAKRMIGGVKQHTDTLATGFYD
                     DKINFKGGDFLKQKATMQLFQKEQHLPSSVIDRDSTRAWKAAGSLDAYGRAKLRVNEL
                     LASYRRPEIDPVKESRLHAFILDLAQKCGLEELPKLEDFQPA"
     gene            complement(43150..44601)
                     /locus_tag="PLE39_01605"
     CDS             complement(43150..44601)
                     /locus_tag="PLE39_01605"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNE67046.1"
                     /translation="MSQKNIKTISNPKLKLEVLTKEDVLKIHNATLQIIEKVGVRFPS
                     KRALEIWESVGAEVDHEKKIVRVKPQVIEDALKKAPPAYKLAARDPQQDLQLDGNHVN
                     LGTDGCGVEVIDIHTGEKRTSCLQDVRDIARVADATEEVAFHWVAVSAQDAPVEARGL
                     HELKAVWENSTKHVQTESIYNVEEAKAAIEMSAAIVGGRDKLRERPILSLMQCTAPPL
                     GHDGGSLDAALLAAEVGIPTGFMTMAACATTGPTTMAGNLAVGNAEVVAATALLQIAH
                     PGAPVFYAAAQTASDLKTGAYTGGGPEDFLFGAATNVLADFYNIPLSMGAFATGAKEP
                     NWQAGLEGAMSSFMASVVMSDMLLGCGFLHGSRIWSFAEMMMDCEIFSIVHKMMQGIE
                     VNDETLAMESIANVGPGGHYLAQKHTRKHMRELFMPQFMDRRPYSEWETKKDDARDWA
                     LAKARKTLKEHQPDMLDERLSQELERIIKSVEK"
     gene            complement(44683..45339)
                     /locus_tag="PLE39_01610"
     CDS             complement(44683..45339)
                     /locus_tag="PLE39_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HNE67047.1"
                     /translation="MSEELYKEMAQSIIDGDSDISTELAKKAIELNMHPLDAITNGFV
                     VGVNYIGDQFGKGEAFLPELVMAGEAMKAAVAVLEPELLKLGSAREMMGKVVLATVEG
                     DIHEIGKTLVGTMLSASGFEVVDLGVDQPADKIIGKALEIDAQIIGMSALLTTTMVRQ
                     REVIEELDKEGLRPRIKVMVGGAPITRDWVTKIKADGYSEDAIGAVKIAKELVGKADA
                     "
     gene            complement(45352..46083)
                     /locus_tag="PLE39_01615"
     CDS             complement(45352..46083)
                     /locus_tag="PLE39_01615"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNE67048.1"
                     /translation="MEKELTHKPLKEEIYDALHRQIIAGKYTPGDWLRQEDIASQMGV
                     SMTPVREAFDLLVAKGIAERVPYRGVRVREISAREVVEAYGLRLMLEALIAREAAENI
                     SPQQLTQIETIMEEMDRHVILSEMPQERQLSREFHAAIAEASGNALMVQVYTIVSNAF
                     PDWLLYEALFRKPELVDESVRQTHDEHAAILDALKKHDADLAVKVSLEHVMESGRWLE
                     THLNIPAKLLKEKEKQITHLLKKPK"
     gene            46247..48133
                     /locus_tag="PLE39_01620"
     CDS             46247..48133
                     /locus_tag="PLE39_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNE67049.1"
                     /translation="MSDIDLNPLKPAIESYIPLGRSGLLPALHAAQKIYGWLPEEVAS
                     EVAKSLRVPLADVHGVIEFYSLYYNEQVGQKFIRVCTDPACALKGADGLLKHLCKNYK
                     VDAGQTTDDLSLTIEQSPCLGLCDQAPAALVDDDAETNISHNFRSYDIGVPQSLVYGS
                     IRELTANCGEGTTTLAKYGEYSAYKKALSVKPEDVIAEVKASGLVGRGGAAFPTGIKW
                     EGAAKASADQKYVICNADESEPGTFKDRVLLLDDPHRTIEGMCIAAYAIGATKGYIYI
                     RGEYPYIVPVLEEALNEARMAGYLGERFDIEIRVGAGAYICGEETALFESIEGKRGFP
                     RVKPPFPTTHGVFGKPTVINNVETMCNVPLIIEKGSAEYRKIGTEKSPGPKLFCVSGD
                     VSKPGVYEVPFGVTLRELLEMAGGVTGKKALQSVLFGGAAGAFATSEHLDVRMTFEDL
                     RAAGLPLGSGVVMVFDETRDMRDVLQRLGHFFAHESCGKCYPCQMGTQRQMEILNRVA
                     AGKTLPDDMIRLQDVGWTMTEASLCGLGQTAASAVLSAMKLWPALFENGGGKTKSDKK
                     SVAAKPRAASARKSVKAKAVKKTTVKKPVAKKTVKKAVKPSKPKAKKTATKKSTANKT
                     AKKR"
     gene            48144..48842
                     /locus_tag="PLE39_01625"
     CDS             48144..48842
                     /locus_tag="PLE39_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNE67050.1"
                     /translation="MTETVNLIIDGQEVSAEQGRTLLEVARAMGKNIPTICFHDATTA
                     NALCRICVVEVEGQRVLQPACIVKAGAGMKVQTRSEKVIRARRTILEMLASTMDLSEA
                     ADIQQMMVEYGASSERFPDAERRESEVKDDNPMYVRDYSKCLLCWRCVQVCAEDAQYT
                     FAINFDGRGFETQIGTFFDKAIPQTSCVMCGQCVGVCPTGALKPKREFLLEQGMSPSE
                     ISVMNTGRKQRKPR"
     gene            48839..51055
                     /gene="fdhF"
                     /locus_tag="PLE39_01630"
     CDS             48839..51055
                     /gene="fdhF"
                     /locus_tag="PLE39_01630"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNE67051.1"
                     /translation="MIKSDRVTTTTCPYCGVGCTLQLHVKDDFIYKVTSPFDSPVNQG
                     NLCVKGRFGYDFIYHPKRTTTPLVRRYLLEGKPKPAGREQVFAVSTDGKPIPDSKISN
                     DWDWVETDWDTALNFVADNLVRIYQRDGSDAMAVYCCAKATNEDNYLLQKMYRALFRT
                     NNVDHCTRLCHAGSVVALQQAVGSSAMSNTASQVIMNDVFIVTGSNTSENHPIIALQM
                     KEAVQKHGAKMIVIDPRRIDLVDMAEMWLPLKPGTNVPVFSAMAHVIVKENLVNHEFI
                     ANRTEGYQDFVESLEKFTPEYAEEISGVPAEEIRKAARMYAGAKNGAIYWGMGISQLS
                     HGTASAMALIHLAFLTGHIGRDGTGLNPLRGQNNVQGASDMGSMPFHYPGYMRVDNPE
                     NRAKWEKAWNIEEGGLSLKLGLTTTEILSHAHEGGVRALYIMGENPMMSEPNLNDTRR
                     HMMELEFLVAQDIFINESGSFADVFLPATPFAEKDGTFSNTDRRVQRVRAAQAPRGMA
                     RPDWQIICDVALRIESRLGRPTAKWDYTNPEQILREMGDVNSDYAGITYDRIDKIGLI
                     YPVPSMDHPGTPTLFKESFPRGRGKFHQLDYVPVREDVDDEYPFILTTGRLLEHWHGG
                     TMTRNSSLNEIYPEARVEIHPADAEIHGIRNDDPVKVQSRRGEVVVRATVTEKTTVGV
                     VFLPWHFHEAAANLLTNDALDPQAKIPEFKACAVQVFPARESDLPNPEIVLNRGRY"
     gene            complement(51124..54456)
                     /locus_tag="PLE39_01635"
     CDS             complement(51124..54456)
                     /locus_tag="PLE39_01635"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNE67052.1"
                     /translation="MAKVFVSYSRKDTEFTKHLTQELQKSEIEFWVDWEGIPPTVDFM
                     KKIENGIEESDAFLFLLSPDSAKSKVCGDEIAHAVKNNKRLIPLMIRDVKEDEVPAAF
                     SHLNWIFFREQDDFDTSFKKLLQGINTDFEWVEAHKRLLIRALEWKNKNKDKSFLLRG
                     QDLQEAESQLALNSSKDPYPTDLHREYILKSRQATDQQRRWTTVFIAIAAVIMFALAV
                     YGLNSARLAVNEAARANESEQREATAKNLALLKETEAKINQLSALAMSKIDQSYNEAL
                     LLSVEAYLHAKDNKINDVNTQSAITKILQSHPGLHQILLGHEDWVNTMVLNPDQTILV
                     SGSWDYTVNIWNMSNPDFPVKLSTLKEFTGPVENVTFTPDGKILATSSDYELILWDIS
                     DPSSPTLLSKTDGYFTNLGFTVDGKILTSLNIHADVNNTIALFDVSNPKLPVELIQLE
                     SGDESLDINSAIFNRNKNILFFTRSDGSIVAWDLQDPNAPTVLSTTDGFGSKVTAIAL
                     SPDENTLALGDINTLIVLWDVTDPATPTIYSNWSGHSLSINSLAFTADGNTLASSSNE
                     TNSQIVLWDVTDRYVPYKIRTINGHSLGVNTVLFNANNNLLFSGSRDGSIMVWDSAQP
                     KTEMEIGAIDGTMIDMAIQPNSNILVSVDSDHKLTSWDISKLSSPVKIKTQDGPASII
                     LFNKDGSLMASMNFDETVTLWDMKDFSKLNTIKLSGETINAIEFAPDGKTLAINTEKA
                     SFWDVSDPKKPVKISTLSQESNEISDMTINADGNLLAGIAANNVILWDISDVTNTKRM
                     GVLEGHSNQVLAAAFSPINDSLLVTAGKDKTIILWNVDDPINPQKMDTLANHSDWISS
                     VAFSPDGTQLASGSYDKYVNLWNISNPEVSVLISKMVGHGGNIGTIDFDPSGVFLASL
                     GNNDNIIFWDITPASWVQKACTITGGDLTDIQWKQYFPTEDYSSTCEPFQTIPEETTL
                     IPSTGGGDTPEFLAACTDSQTPSCSEPTYEITDLFCIDRGSYGLYTLSPGTTFEVLTP
                     GFNCINEDMNSDGEPRISCTGPADTNFLVSFCNSACTNSLETSYQCQAGFGLNAAEGC
                     CAPVTSASNGCITQTLALPECE"
     gene            complement(54554..55726)
                     /locus_tag="PLE39_01640"
     CDS             complement(54554..55726)
                     /locus_tag="PLE39_01640"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNE67053.1"
                     /translation="MKDARFNQYFQKSFLLQIITAWTVLVLAVYFIHENWLQNNLPAF
                     ALLWAVGILGLQTSIRWRWNNLNDEEKDFEARKISNLIYHAVMNTSTDSIAVVDPFGN
                     YIFANKQTAVLHGYSSSEEFIGVSAFKFFSLDEVARAIKYMEMTRADGVIRNIEFNLL
                     RKDGSTFPAELSAALVKNELGLPVAFIAVTRDITERKRITDQLRDVNEQLRKQLGEIE
                     KLQSILREQAIQDPLTGLYNRRYMEEVLKQEFARASREGKPFSIVMLDLDNLKTLNDT
                     HGHVVGDEALKLLARQIKRLTRIEDTACRYGGDEFLVILHNADEMTAQNRIEELTKRI
                     QEARITSVGEEMPISFSAGSATYPRNGDTVEQVIQFADTALYNVKASRKKPRVTSQ"
     gene            55777..56418
                     /locus_tag="PLE39_01645"
     CDS             55777..56418
                     /locus_tag="PLE39_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNE67054.1"
                     /translation="MTRNYLVPLTEIRREQEVLKSRFIATLAPAFSIDEARAFMARMK
                     KEFSDASHNVPVYVIGGGNTVTEYFSDDGEPSGTSGKPALAVLRGSGLGDVVLVITRY
                     FGGTLLGTGGLVKAYTEAAQSVVNAVERGRRVPVHVVMMAIPYNLLERVRLLVSKDRG
                     KILGEDYAGDITLTMQFPVENFDTFQADMRELSAGKIQAEVIESKEEIVSASA"
     gene            complement(56367..57590)
                     /locus_tag="PLE39_01650"
     CDS             complement(56367..57590)
                     /locus_tag="PLE39_01650"
                     /inference="COORDINATES: protein motif:HMM:NF027579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HNE67055.1"
                     /translation="MDYVQRAFEHIRHLSETIGGRGSCSPNHRQAAEYSAEALRSAEV
                     SQIEFEPFRGMASTYLPFALAFASALTGTCLALFFGNRLTFGIAALMNFLGAWAMFAE
                     SEFASNWTRWLIPTAETINVTGAVSPQTKPKRQIILCSHLDTHRTPIFYSSTAWQKAF
                     GVSVTGAFLSMVLSALTFGLGTLLDLNEFRWVGLLFGLVQMYALILVISAEFTPFSPG
                     ANDNASGTGIILALAERLRAEPLTNTKVNFIFTDCEETGAHGILAYLKKHAEAFGKDA
                     VYIILDEVGAGQLKTVTEDGLIRKSKTHPRALELARNANFGLTQSLIEAKGEAYTDAL
                     PATKQGLIALPISSAFPNPEQAVSHWHQMSDRIEFIDRQSLQDTFTFTWNILQAVDHQ
                     ADAETISSFDSITSA"
     gene            complement(57607..>60424)
                     /locus_tag="PLE39_01655"
     CDS             complement(57607..>60424)
                     /locus_tag="PLE39_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HNE67056.1"
                     /translation="SYNMPMNLTTILPASRIRDRLIDRIAYANDASYFRLVPQAVVQP
                     NSIGEIQSLFKFSQQNKIPMTFRAAGTSLSGQAVTDGILVDISKHWGEFSVEADVNRI
                     RFQPGIVGGFLNNVLKPHRRRIGPDPASIDACMMGGILANNSSGMCCGTTENSYKTIH
                     SMTFVLPNGFVLDTTDPNANQLFENEQPQIAKGLLELKKRVTSNLKLSTRIAKRYQIK
                     NNNGYLLNAFLDFNSPLDILVHLLIGSEGTLGFIAEGVLHTLPDFTHKYTGQMYFKNI
                     RDAAAAIYPIKQSGARAAEIMDRESLRAVENNAGVPAILKQLPNGAAAILVEYQAADS
                     ESMLQFKKEAQRVIGKLKLIHDVEFTDDPIQQAALWKPRKGIIASVGAMRPRGTTSVN
                     EDVAFPVEHLADAVTDLRHLFDDFGYTEGVIFGHAKDGNLHFLVNQAFDTPEQINHFD
                     NFMQAMVKIVSGKYDGSLKAEHGTGRNMAPFVETEWGSEAYAIMRDLKSLLDPDGMLN
                     PNVLINDDPKAHVTHLKSLSLVAPEIDKCIECGFCESKCPSRRLTLTPRQRIVVQREL
                     ARMRLADSSSPEFDSVSTDYTYAGIDTCALDGLCGTACPVGIDTGKFIKKLREKRVTS
                     NKSAEWIVENFATVEKAVGIGVSLGQAAEKVIGAIGVKSISAAAEKVTGTRLPKWNHS
                     IPHSPKKLSALRGEKEFIYFPSCISRQLGKPTHHSPLSTVFLTIAKRANINLQIPDQT
                     EGHCCGMPFSSKGYKPAYQAALHKTLMQMWEWSEHGKYPIVIDTTSCTHTLKTCGEDL
                     TPEDREIWRQLTLLDGVEFLHDHVLPKLEIHPVNEEVILHPNCSARKLNLDVKMHAIA
                     RQCARSAVVPFALGCCAFAGDRGLTHPELTASATEKESAEVLAKEYGGHYSSNITCEI
                     GMKEATGKDYVSIVYLVEKASRKI"
BASE COUNT        14036 a        14900 c        15632 g        15756 t
ORIGIN      
        1 gtttctagta ggtcattttc ttttgcagca aacgtgaatc cagcggaatg gttgccaacg
       61 cttctgcgat ctttggtcct gcattgccag cgccgtatgg attcttgcag gtctggcatt
      121 gatgcctgaa tccttcatca tggatgctac gctgaatggc cgcaaggata ttttcttcgt
      181 tataggaagt atccaaaaca ttatcagcgc gtaaacgtcc ctgctgcctg ctgccgatat
      241 tgactgcggg acaaccgaag gctggggttt ccttgatgat ggccgaagaa ttaccgacaa
      301 aagccccgcg tccgacccgc cctatgatgt tcagtacacc gtgaaagcgg tggcgcccga
      361 ggtttttcgt gatgtgcatg ttgggcgttc ctttcagctt ttcgatctca tcaataatgc
      421 ggcgtccacc tgcatcgtta ttgggatagg tgatgatgat ctggtttcct tcactagcga
      481 gtttttgcaa agcccgcaaa gatggcttca cctgttcggc ggcctgctca aattctgttg
      541 taaccgaatg ctgacagaaa agaatgacag gctttgaaag gtcaagttta agttcggcgg
      601 caacctcttc cggggaggca aataaccccg cggtgatcaa gtccagagaa ggttgcccaa
      661 cgttgaaaac tctccagggt tcttcaccca gcttacagat gcgttcgacc gcctgttcat
      721 ttgtagcgaa atgtaaatgt gcaagtttcg tcatcgcatg ccgcactgag tcgtctaatg
      781 caccaccttc ggtatagtcg ccaccctcga tgtgtgctgt tggtatgttc atctgtgtcc
      841 cggtgatcat cgcggaaaac ccttcgtaac ggtcagcgta gacaaccaga aaatcaggac
      901 gcagttcatc taggattttg ctcagactga cgatgcctgt gccgatggct tgtgctgttg
      961 aaaaaagtgt gtcgtgctcc attcgaattt caacttcacg atacactcgg aatccatctg
     1021 cttcgatctc agccattgtt ctgccgaaat cctgttgcag atgtgcccct cctgccagta
     1081 aaaaataatc caaccgtggg tcggcggcaa tggcgcgcag gattgggtac tgcaatccat
     1141 actctgcgcg attcccggta aagacagcaa tttttctttt tgtcatattt tcatcccatt
     1201 tcaagtagtg tttggcgaag accggcctct aaagaccatt gaggctgcca ttgggtgtgc
     1261 gtttttagct tttgaacgga aagcaatatt ctatcttcaa cacccgatga aggaggttcg
     1321 gtttccgtta cacttatttt ctgtttaccc agcaggctgg cggtttcagc cagttcacgg
     1381 atgctggttg ggatgccaga tgaaagattg aatacctcac aaccagattc actggcatct
     1441 ttgcccagtg cgatcaaacc atgcacaaca tcatcacggt aaacaaaatc tcggataggg
     1501 gaaagggtct tgatcgaaat tggaccaccg cttctgactt gctgtaagat gatcccgata
     1561 actgaatcag tggagccacc cgggccatat acattcccga ggcgtgctat gcggcaggaa
     1621 aacccatgtt cggcggcgta tcctgcgagc agatcttctg ctgcaagctt tgtggcggca
     1681 tagattgagt tcgcctgtac agcatctgac tctgtgaatg gcaggttggg cgtttttgca
     1741 tacaccaaca ctgttgacgg aaagacgatc ctttgaatgc caatcctgcg acaagcctct
     1801 agaagattgg ctgtacccgt cacattggag gtaactgctg acattgggtc aagccgagat
     1861 cttgccacat gtgcctgccc tgcaaggtga aaaacgacca tttctttttg ggtagttttg
     1921 ctaatgatgc cagacagatt gttgaaatca caaatatcag cttgttggta tttaacgcct
     1981 tcaggcagaa acttgggtgc ggcttgcata tccagcccgg aaacggttgc gccttcctga
     2041 agcagttgct ttgcaagcgc ccgcccgagg aatccaccaa caccggtgat caaggcgttt
     2101 ttgatttcca tgccttactc aaaatcgtcc caactcaatt gggaattttt catgattgtt
     2161 tttttagcgg ttttaccaag cagattttca aaatgaactg ctttaatgtc acctgtcccc
     2221 ggacgtttga cccaaatgtt ttcagcagac agcttttccc ctggctgaat gtctttgatg
     2281 gaaacaacac aggcataggc aaagtcgatc gtcggttttt cttccgcaag aatggtcttg
     2341 gaaccaccca acgctgcatg gatcgcgcgg gagccttcca ccaattcctt taattcaatt
     2401 gggtcaatgg agatggggac gtcgggtccg ggccaggtct tatcggaggt aaagtgtttc
     2461 tccaaaatgc gcgcaccgag agctacagcg gcaaaacagg tgtagtttcc caatgagtgg
     2521 tcgcttaagc cgaccacggc atctgggaat ttttttgcaa ggtcagccag cccgccaagt
     2581 cgaacttttt cgtagggggt gggatacatg gatgtgcagt gtgttagtgc aaatggcaca
     2641 cctgcctggc gcaaaatatc aaccgcaggc gcaatggatt cgagatcgtt catgccagtg
     2701 cttagaatta tgggcttgcc atcggctgca atatgcttga tcaacggata gttgttgcat
     2761 tcccccgaac cgatcttgta tccgctgacc cccatcttct tcaggcgttc ggcagcagcg
     2821 cgtgaaaaag gagtacacaa gtagagcatt cccaaagatt caacatagtc tttgatcttg
     2881 atctcttcct cttcggagag tgcgcaacgt accatgatat cccagatggt ttctgtggtg
     2941 ttgcctggaa cgacatcgtt ggggatcatt tcgtcatcca ccacatgtga ttggaattta
     3001 acacactcac agcccgctgc atgggcatca tcgatcatac ggatcgcttt ttcataactg
     3061 ccttcgtggt taatgcctat ttctgcaatt accagcggcg ggtgttccat gccgatcata
     3121 cggttgccaa ttttgaagct catgattaat tcctttcaaa aataattagg tcggtgttga
     3181 tttggaattg tggttgtatt ttccatcttt ggcgtcattt tgagcgcgtt cgtaatcggc
     3241 tggctggccg atatcgagcc agtattctat gattgggaag ctgaccacgt ttctttttgc
     3301 atcgatcagc cgttgaataa gatcggtcat gtccatacgc tcaccttttg caatgaggtt
     3361 gtagaccgat ggctcgagga ggtaaatccc tgcattaacg aggaacgaaa tttgcggctt
     3421 ttcacgcaaa ctgacaatat tcggaccatc acaatcaatt acaccatatg gaacctggac
     3481 gccatattgg cgcacgccaa cagttagttc tgcatgttgt tttttgtgat atgaaagcat
     3541 gacttccaga tccacgtgtg tgatgatatc gccgttaatg accagcaagg ttttttccgg
     3601 ggtatccatc agacccaatg cacctgcagt gccgagtgga cggtcttcct gtaagtagtt
     3661 tagttcgatc ccgaacgctt ttccatctcc aaaatgctca acgatgttgg atgcacgata
     3721 attggtcgat acatccacat gttcaatgcc cgctttttgc agtttctcaa tggtcagttc
     3781 cattagcgga cgcccgccaa caggcagcat gggttttgga atattgtctg ttaatggacg
     3841 caggcgtgtc cccagcccgc cagccatgac aacagcctgt agcggtaatt tgggttggga
     3901 cagtatttcg ctcaggataa cgatatccac aaccctgccg tctttatcca gcaggggaat
     3961 ctgcttgacc gcatgctcct gcataatgga cagaaattgc gatgggtcgg tgtccacagg
     4021 ggccgtaaca ggtattgggt gtggcatttg agctttgatt ggcaaaagct tgctgacggg
     4081 ctcgttcagg ctgatacctg ctagaattcc gcggcggaca tcgccatcag taatggtgcc
     4141 gagcatgcgt tgttcatcat ccacaattaa ggcaatacta atgcggccgc tacggtcaat
     4201 acattctatg acttcacgga tagaagtgcc caattttacg aacaggttgg agatgtttgg
     4261 ataggtcata cgggcaccaa ataattacca ggctgtccag ccgccatcca cagcaaggtt
     4321 ttgacctgtc acataagcag ataggtcact ggctaaatat gcagcagcgc ctttgaaatc
     4381 ctcttccatg cccatacgtt tcagcggcgt gcgttcttcg taacgcttta caaaaagttc
     4441 gggttgattg cgccatacac cgcccggtga gatggcgttg acccgaacat gcggcgccat
     4501 gactgtagcc agccaacggg ttagctgtaa taaaccacct ttgctggcgc cataagcagc
     4561 agcatttccc aaaggcgttt cgtcgtaaag gcgcatatct ggaccgacca tgccataaat
     4621 ggaactgaca ttaatgaccg agccatgtcc ggaagctttt aatgcatctg caaatacttg
     4681 tgtcagaatg aatggggctg tgaggtttac ttccaaagcg agccgccagg cctctgcacc
     4741 ttgttcctga aatggcgcga cccagccctt taaattagat gtattgacca tggcagcgca
     4801 attcacaagg atatcgagcc tgccgaattt tgtcagtact ttttctggcg cgctacgaac
     4861 cgaattctcg tcggcgaggt caatggacaa tggcatggtt tggggcccga attgctgctg
     4921 aattttattt gccacttcag cgcagacgct tggagaagag tccagaacga caatgtttgc
     4981 gcccagttct gccagggctt cgccgattgc cgacccaata tgccctgcgc caccggtaat
     5041 taatgctgta cgaccgtcaa gattcatgag ttcttttaat gatttcatta aggctccact
     5101 ttacagatca ccgcttaatt taaaataaaa actgcatgtg caagaagtaa acaataatta
     5161 tttgtcatta tcgaacaaat gcatgttttc ccatatttcc tcccgtggaa ggaaggggaa
     5221 catatcttcg agtggtcgtg attcaatggt tccatcttcg cgcacgaaag aagatgcctt
     5281 tggtgtaata ccctgttctg gcgaaatatt aacctcgcaa agaacagggc cgttcagcgt
     5341 tagtgtttcc tgaataccct gttcaatacc atcattgttt ttaatggata cgtattccag
     5401 accataggct tttgcaagat actcaaagtt tgggttgctg acaccggaac tttcgtcaga
     5461 acctacaaag cggccgttgt aaaaagtctt ttgcgtagcg cgaatgctgg aatagccccg
     5521 attattgaag aggaatattt tgaccggtag attgtaattt tttattgtaa gcagttcctg
     5581 tacattccat tgtaaactgc catccccagt aacacagatg gtgcgctttt tgtcattgcc
     5641 aatacaggtg ccgactgcca gcggcaggtc ccagcccatg gcgccccagt taatactggt
     5701 gtaggttcgc tggccttctt tgactttgaa tacttgaagg taacttactg tgtcgaggcc
     5761 attaccagcc accagcgcat cggtattcga aagcaggtcg gacaatttgt ctatgaatac
     5821 gtagctgttg acgaaatctt tgtccgtgta gtattcatcc acaatcagtg gatatcgttc
     5881 cttccatcgg agacaggctt caagccattt ggcggaaggt tcaactttga cagtttgcaa
     5941 ctgcccaagc atttcttcca tgaaaaaacg gacatcggtt tgaatcggaa ggtcaggtgt
     6001 gagcacctga tggaataact gaccttcgtc aatatcgacg atgatcttct ttgcatttgg
     6061 cgcaaagcct ttgtaattga atcccacctt ttttatgcac aaccccgccc caaggctgag
     6121 aatgcagtcg ctgttttgaa ctgcaaaatt ggcccttcgt tgacctgccg ttccgaagat
     6181 ccccatataa agctggtgct cttcagcgac taggtctttg gctgcgtcag gcaacacaac
     6241 tgggatattc aacttttcga taagcctttt cagtagttcg tctgatttgg agaggtgaat
     6301 tccatttcca cacaaaagca acggcctttt ggcgtttttt atcatctcaa gggcattttt
     6361 tacgtcgctc gtcaattgtg tgccttgact tagtttttca tctactttag ggtgatagga
     6421 tggcaattga gattcttcaa cttgactgtc ttgaatatcc aatgggatat ccagccagac
     6481 ggggccgggg cgtccactga tggcttggtg gtaggccagt tccaattcgt acttgatcat
     6541 atttgcatca agaacaacat tggcatattt ggtcacgggc ttgaccatcg ccagattatt
     6601 gccttcttgc gggccgatct ggcgcacttt ggaataatcg gcaataatgt ttcgccgtac
     6661 ctggccgcaa atgacaatca tggggatgga gtcgtaccat gcgcccacca tgcctgaaat
     6721 tgcatttgag ccgcccggac caacggtaac cagacatgca ccgacgtcat tcctaaccct
     6781 tgcatagcct tctgcactga tcgcacacgc ttgttcgtga taattgcagt aataattcat
     6841 atcgggattt cgaccaacgg cgtcaagcat gtgcataatt ccaccgcccg aaacgaggaa
     6901 aatatcgcgg ataccttttt caaccaggaa tttgacaatg taatcggaaa ctttgatcat
     6961 gaatactctc cttgtttaat tataaagcgt accattgtgc ggtgcgttgg attgcatccg
     7021 gtaattttgt aaactcttgc aggcccaatt cggtctttgc gcgttgggtc gatggaacat
     7081 atcgttcagc ggttttgttg ggggtggggc gtttggcaac ctggatgggc agctcagggg
     7141 cgatgacatc agcaaccgtt tttgccaatt gaagaatgat gaggtcgttt tctgagccga
     7201 cattgtaagg gtgacaggat tgtccgcgca ggaggatcgt ccatagccag atggcaaggt
     7261 cagctgcgta tagatatgaa cggtatgggg tgccatcacc gccgatttga atggaggtac
     7321 tgctcatcgc atcgcggatg aaatttccga tggcatagtt gctgtttaac ggcaggcgcg
     7381 gaccgacaaa tgcgaagcag cgagcaatcg cagcatcgaa tccatgttgc ttggcgtagg
     7441 cggcgcacaa aaattcagag gcacgtttgc tctgtccata ggcggaattt gtgtcggttg
     7501 gttcgggtgc gccaagatag gtttcaggga tatgggtgat ctcggcgggt tgttttccgt
     7561 acactgcgcc tgaacttgtg aataggaatt tttctgcgcc cgatttgacg gcgaaatcca
     7621 gcacatgacg agtcccttcg atgttggatg aaaatttttc caatgggtca acgtctgtaa
     7681 aatctggatt tgtttccgtg gcggcgtgga tgatgtgcga gaaatttccg cgggggaatt
     7741 tgaaatcacg tgcattacct ttgatgaggg taatggcagg gtcaagccca aggtgaggaa
     7801 atgctttttg gaaggaagca ggtttacggc taaggaccga tgcggatgct tttaaatcca
     7861 attgacgatt tgcaaaaaga aaactttcga gcagccatgt cccaaagaat cccgttccgc
     7921 ctgtgatgaa aagacgcgca ccacgcaggt cttcccacag gttgcgggta tgatcgagga
     7981 tgtgaccaag atcagtggag ggaagatttt gcatcatgaa gggcttcaac tacataatcg
     8041 agcatggggc gggtcagtcc ggggtaaaca ccgatccaaa aaacattgtg catcacctcg
     8101 tctgattttt tcagatcgcc gatgatacga taattttggt ttcgataggc aggctggcga
     8161 atcaggtttc cgccaaacag gagccttgtg ccgatcttcc gtgcttcgag cgagcgtaga
     8221 attgcatcac gtgtgaagtt tttgttttcg cgaacaaaga tcggaaaacc aaaccaactt
     8281 ggatttgagt tttgtgttgc ctctggcaga acgaaatggt cttgcagcgg gcttaactgt
     8341 tctttgagat agttgaaatt ctcgcgcctt ttttcgataa agccatttag ttttttgatc
     8401 tgcgaaacgc ccacggcagc ttgcatgtct gtagctttga ggttgtagcc gatgtgtgag
     8461 taggtatatt tgtgatcgta gccttgcggc agatcgccga gctgccaatc aaagcgtttg
     8521 ccgcaggtat tgtctttgcc tggctcacac cagcaatcgc ggccccaatc gcggaatgat
     8581 tcaatgatct ttttcaattt gggtttgtcg gtgagtaatg cgccgccttc acccatggta
     8641 atgtgatgtg ctggataaaa actaacggtc gctacatcgc cgaaagttgc caccattcga
     8701 ttggcatagg ttgcgccgac ggcatcacag caatcttcca ccaaccagag gttgtgctgc
     8761 ttacaaaagg cagtcactac ttcaaggttg aaaggattgc caagtgtgtg ggcaatcatt
     8821 acggcacggg ttttttgcga aagcgcggct tcaagttgat ttacgtcaat gttgtaggtg
     8881 ggaatatcca catccacaaa aactggaacg aggttatttt gaaagatcgg gttgaccgtt
     8941 gtcggaaacc ccgttgccac agttatgact tcgtcacctg gtttcaattg cctttctttc
     9001 aacaatggag aagtaaggca ggtgagtgcc agtaaatttg ccgacgaacc tgaattcacc
     9061 aaaatggaat ggcgcatgtt gaagaagcgt gcaaaatcct tttcgaattg ccgcgcaaaa
     9121 cggccttctg ttaaccagaa atcaaggctg gaatcgacca atgattgcac gtcggctgaa
     9181 tcgaatacgc gtcccgccac aggcacggaa gatacccctg ggacgaattt gcgttcgggg
     9241 aattcttcgg cggcatattc gaccgttaat tccagaattt ttgcgcgcag ttgctcggat
     9301 ctctcactca tgtttgaaaa aatccttgta ccagttaatg gttaatgcca gcgcgtcatc
     9361 aaggttgaaa agcggtttcc aattcaatac gcggcgggct ttttctgcgc taaggtattg
     9421 atgacggatc tcgttactgg tctcattgag gatttcaggt ttaagatcgg aattcatcaa
     9481 ctccaggaca tgatccacga tctcgcgcac tgatacttga atctcgttcg aaaagttgaa
     9541 ggcttcacct ttgagttcct tgcgggtggc gagttgttca gccagcaaca tgtaggctgc
     9601 cgcgccatct tccacgtaaa aataatcacg gatgtattcg ccgtcggagc ggatgatcgg
     9661 acgctgtccg cgcaaaatgg agcggatcgt tcctggaata atacgattcc agttcaagtc
     9721 accgccgcca tagaagtttc cacagcgggt tatggcaaca ggcaggtcga aactttttgc
     9781 ataagtatgg gcgatcaggt ctgcagctga tttgctgaca tcatatggat gttgtccttg
     9841 cagcggggtc ttttcatcgt acggcagaat ctcctgatcg ccataggcct tgtcggagga
     9901 tgccatgaca acctgcttta cttttgggct gcgtcgggct gcttcgagaa tattccatgt
     9961 gccagcaata ttggtctcaa atgtggagac cgggttacga ttcgcaatgg ttacgatggt
    10021 ctgagcagca aggtggatga cggtgtcaat ttcaaattcg ccgagtgtgc gttcgagcat
    10081 atcgcggtca cgaatatccc cgcggacaac ttttaccttc tcaatataac ctgcacggac
    10141 catttcactt tgcggcaccc aatcgcggac aaggcagact acatctgcac ctgcggcaag
    10201 aagccttttt accagccatg tcccaaccag gcctgtgcct cctgtgacaa atgtggggcg
    10261 gtccagccaa aaggctttgt ttaattccat agtttccatg gggcattacc ttcctgccag
    10321 agcttttcga gattatgaac gtcgcgaacg gtgtccatac attgccaaaa gtcttcatgc
    10381 tgataggctg aaagctgctt gtcagccgca atatgctcca aagactcaaa ttcccaggct
    10441 gtgtggtcac cttgaatgta ttgggtaata ccaggctcca aaacaaaaaa accgccgttg
    10501 atccaacctt caccgatttg cggtttttcc ttgaattgga cgacgcggtg atcttcgata
    10561 accatttgtc caaaacgtgc aggcggacga actgcagtca ttgtgaccag ccttttttgc
    10621 tgcttgtgaa aatctatcag gtttggaata ttcacattgc tgacaccatc accataggtg
    10681 agcatgaagg tctcgttgcc aatgaactct gccacgcgtt tgacacgtcc gccagtgttg
    10741 gtgtcggcac cagtgtccag cagatgtacg gtccagtctt cgccttcgat gttatgggcg
    10801 gttacacccc ctgttttcag ttggacggtg atacttcgtg atcggtagtg ataattaaga
    10861 aaataatcct tgatgatctc gcctttatac cccagtgcca ctacgaattc tttgaaccca
    10921 tatgcggcat aaatgttcat aatatgccag aggatgggct ttcctccaac ctcgaccatc
    10981 ggtttcggct ttacagttgt ttcttcagat aaccgcgtcc ccaatccacc tgccaggatc
    11041 gcaaccttca taatgaataa atcctttcaa agaaagatgt gcctgattat acatgtactt
    11101 gaagaccctt ttgttcacag aattgtaatt gaaatattgg tcatatgatg gatatacaca
    11161 aaaaaaacga ctgccatttt cggcagtcgt tttttttgtg ggcacggagg ggctcgaacc
    11221 cccgacctca tgtgtgtgat acatgcgctc taaccaactg agctacgcgc ccggaacgac
    11281 ccgcattata gtatagcctt gcgcctttgg caaggactta cggcggcggc aaataatccc
    11341 agggattgac cttccaggtg gcggtcatta actcaaaatg gaggtgtgga cccgaagatc
    11401 gcccggtgga acctactgca ccgatggctt caccctgccc aacgctctgt ccgcaacctc
    11461 tatagatcgc actcaagtgg gcatacaggg attgaaaacc atttccatgg tcgatcatga
    11521 tcatattgcc gtaaccgtag ttgttccagc cagagtacac aaccacacca gcgtcggcag
    11581 catacacacc ttcaccttcg ctgcctgcca ggtcaatacc gcgatgattt gtcttttcgt
    11641 tgtagtcaaa acctgagagg aaatgtttgt tcgttgggta cacataagtg ccatacccaa
    11701 ccgcgccacc gctgaccggt tcgcaggcgc cctgaccaag tacacgggca gaagcggggt
    11761 tttcacgtgt cacacccagt ggcgcgctcc aattaacaaa ctcccgtttc ccgttgggaa
    11821 tgatcagcca tgtgccggtc ttgatatttg cgtttgcata gtcgccgacc gcattcgggt
    11881 ctattttgtt gctcgggtat tcaataatgt ccgcagcggt gactccgtaa aactcagccc
    11941 agtcgccaaa gggaatgccc cccagccact cccaatacac gccgtccacg ggcagaatat
    12001 tcaactcctg cccgggcttt agtgagtggg gatcgtccaa taaaatgttg taattacccc
    12061 aaagaatggt ttgaggttgc aggccaaatt tttcagcaat gccgaaaaca gtgtcaccct
    12121 cttgcaccgt atagtttgtg atctcattgc gtggtcgcga gggaatgttt gtatgtatct
    12181 gtgccgaacg ggaaatcccg gtaattagtg gattttccaa tgcggggatc gaatggatat
    12241 ccacttcaac ggtcggggcg ggacctgaag cctggtcgct gtttcctgaa gcaggattcg
    12301 cccgttgggt gttgaaggcc tgtacaagca agatcacaac aacgatggcc gcaatggaga
    12361 aaaggtttgt tccactgcgt aacaatgatt ctcccagccc catttgaacc agtgtattca
    12421 tccaccgtgt gaaaaaacta cgctgtctta caggctcagc ctgtttggct tcctttggct
    12481 tcgccgagct tgttgaatct tcattaaaat cgttttgcat attgttcatc ataacatgct
    12541 taaaaaatca cagtcaagtt gccttttaaa cgctaatcct gtattaacca gaattcgggt
    12601 gtttattgaa cgggaaccac taattcgggg cggtcattgc ctggagcatt gaccatggtc
    12661 gaaacagggt aggcggacat tttgtcagat gggaaagacc tgatgaggtg gagcaggcta
    12721 tcgggagtcc gaggcgcggc gtccagccaa tcggcatagt tttgagcatc cagaatgacg
    12781 ggcatgcggt tatgcagggt ggacatcagt tcgttaggtt cagtggtgat gatggtgcag
    12841 gtgcggaccg agccgccatc gggagaatgc cattcgtccc acaaacctgc gaatgcgaac
    12901 ggcttgcggt ctttcatgtg gatgaagtag ggcgttttgg tttttgcgcc aggttgtgat
    12961 ttccactcgt aaaatccgtc ggtgaggatg aggcagcgtt tgtatttaaa actaccgcga
    13021 aaagatggtt tttcggcaat ggtttcaccg cgcgcattga tcaatttgtt ggcgatggat
    13081 gggtcttttg cccatgaggg aatcaacccc cagagaaaaa aatcggcctt gttctttgcg
    13141 tcgtttggaa tggcgaggac gggctgtgtt ggggcaatat taaatcgcgg tgaaaactgg
    13201 gtgggaaagg tgaattcaga aaaagtatct tgtaactctg cagggtcaac cgtcagggta
    13261 aatcgtccgc acatggtaag ctcctttttt tatgaaagcg taaaataaaa agtggccccc
    13321 cggttaattt gagaatcgac ccaaatgcgc ccgccatgca cttcgatgat cctttttacg
    13381 agcgcgaggc caatgccagt gccgctggat tcggtggaaa gtttattgaa caggccgaat
    13441 attttttcgt ggaattcggg cgcaatgcct gcgccgttgt cttttacgta gaaggtggcc
    13501 tgtccgcgtt cgttttcgaa agctccaatt tcgatgcgtg gcgagggttg gttatccatg
    13561 aatttgatgg cgttttcgat caggttttgc agcacttccg tcaaacgtac ccgatcgcag
    13621 tggatcatga taccggtttg cttgtgaata atgtgaatgc tttttccttc aatctgccca
    13681 tggacgattt ccagtgcatc tttgacaatt tcgtcaaatt ggatatcggc gggtgtgttg
    13741 atcaacctgc caatgcgcga tagctcaagc aggtctctga gcaggttttg catttttcct
    13801 actgcggttt cgatgcgcga gacatcctgc ttgaatttgt cgaagtttcc cgaaagggca
    13861 tcctgttcaa gataacctag aaaccctttg atggtgacca gcggcgattt taggtcatga
    13921 gagacggtgt aggtgaatct ttccagttcc gcgttctttg cttcaagttc ttcgatgaag
    13981 gtcttgcgtt gggtaagttc gttttgtagt gcagtgttca acctggcgtt ttcgatggcg
    14041 agcccggcat agcctgcaaa caagcgcaag gcttcaagct gctcatcaga tatgggtcgt
    14101 ccgctgattt tgtggtctac acagatgact gccacgggtt tttccccgac ccaggcggcg
    14161 acagcggcaa agtctttaac gccgagcatt atgtcattta tggcagactt gtgctctgat
    14221 tcgtaatcac gagttaaata gatggaatca ggattgttga gcagatacaa aaaggaattt
    14281 gcttcctcgt ttccctggtc aatggatttc caggtttccc attcgtcaac catctctccg
    14341 cggtcgttgg tgccaaacgt gccatccatt gacttcctga cagggttgta catgtagatt
    14401 ccaagacggt caaatgccag ttcgtcatgg actccgtgcc aaattctttc gagggtggca
    14461 cgcagattgt ggacctcggt catgctttgc ccaagttgaa tgactttttc cagggttgca
    14521 cgacggcgag cctcacgctg ttccgcactt ttttgcaggt ctaattcatt ctgaatggcg
    14581 gaatgcagac gtgaattttc gatggcaagc cctgcatacc ctgcaaataa acgcagacct
    14641 tcgagctgtt cgttcgtgat ggggcggagg gtgataaggt tatctgcaca gataaccgcc
    14701 acaggtttgt tgcccgccca aaccgccaca gctgtgtact ctttgacatg gtacatttca
    14761 tgtccgggag gaatgttatt ctctttggca taatctttcg tgaagtaaaa accatctggt
    14821 ttttcgagca ggtaggcaaa agtcgagccg tctgtgtctt cccctacagg gaaggacagt
    14881 ccgtagttat ctgtcatttg gccgtttatg tcggtcccca gcgtatcgtc catctggttg
    14941 cgttcagcgt tatataggaa gatcgccaga cggtcaaatt gcagatcgtc gtgaacgcca
    15001 tgccatatct tttttatggt ccctttgatg tcgctggctt cggtgacgag tttgccgagt
    15061 tcgatcactt tttcgagcat ttcgcggcgg cgtttttcgg caagctctgc ttctttgcgg
    15121 tcgttgatgt cgcgtgccac cccggttgtg ccgatgacct ctcccttgcc attgaaaatg
    15181 ggggttttga tggtttccac ccaatattcc ctgccaaggc ggtccatttg cttttcttcc
    15241 acccgcctta atttcagggt ttcgatcaca agcaagtcat cttcacggta atttttggca
    15301 aactccggat tccatatttc atcatcgctt ttgccgatga tctcttccat gggccgcccc
    15361 gcaacggacg caaatttttc attgacggcc acgtatttgc tgttcacgtc cttcaaccag
    15421 gccatatcag gaatcttccg aaccatttct gcggtttggc tgagtgttgc gatcaaggtt
    15481 tcttcggcgt gtttgctttc agtaatatcc tgcactgcac cgataatgcc agccagtctg
    15541 ttgttgtaat catcccagat cgggtgggcg tagatccgct cccagtgcgt ttcaccattt
    15601 ttgttgatgg tgcggatttc agattttata tcctcgtttc tggcaagtgc ttccatatcc
    15661 cgaatgtctt tttcgatgtc gtccgggtga acgtgcgccg accagctacc tgctgcaagg
    15721 tattcctcaa gcgtgtaacc tgtcattttt tcaaatgcgc cgccaaccca aaacagggtg
    15781 ttgttccccg cttcatccac tttggtttca aagacatagt ctgtgctgat gtttgaaagc
    15841 agcatgtatt ttcgttcgct gatgcgggca cgttttaggg cattttgcac agaaagactg
    15901 gaaaggtatt gcagtgttgt aatgatcgga aaaataacca gacttatcac ccacatattc
    15961 atggagcggg tgagatttgt tgaaagaatg gcctgctggg tctctgcgta gatcaatacc
    16021 aacccagtca agagcgaggc taccgtcacg tacgaggctg ctctcccgcc gaatagaacc
    16081 cccgcaatca aaattaccaa cgggaatccc attaagtaag cttcgctctg caccccttca
    16141 ccggaaaccg cagtgatgga aaagaagatc catagcatga caatctggag tatggctgcc
    16201 aggcggacga tcccttttct aagcatgaac agaagcaaaa ggttgatcgc ctctccaaag
    16261 gctgattgcg ccagcacccg attgaatccc gaaggattgg tgatcgcaat aaatattaaa
    16321 tagggaattg ggacaaggat catgccccac acaatggcgc gcaacaagaa tgcctggtga
    16381 gacttttctt cgctatcgaa agtgggtggc ttgaatgagt tacccataaa aaaaattgga
    16441 ggtatgcttt tagttcctca atttgcgcgc atcaccgtcg cggatggtga tgttaatggc
    16501 gtccatgtat tcgaggatgg cttgtgcata ttttctggtt gtaccgagca ggtcgcgcac
    16561 ttctgcaaga gtgatcgttc ctttgttctt cagctcggct cgaattttga tcacaatcag
    16621 ttcatggtct tgttttttga aaaccacatc tggtgacacg ctcaccagtt cgttcatttc
    16681 aagcagcgca ttgaatgcct cttcaccaac ttctgattga cattccttga cgcttggcgg
    16741 accaaacgga ttcgcctcga atttacgcat caacccatta acccttgctt gatcctgtcc
    16801 attgaatttt atctcatggg tggtttgtgc caccgaaccg cgcaaatccg tcagcgctga
    16861 atggtgggtg agaaaattga ccgtggcatt aaagacacgt ggagtgagtt ttagcctgct
    16921 ttttaactct tcgcgtggaa ttccacgccg taatggaaat tgctggtgat acgttttaac
    16981 gagttgaagg gttttctcct gcaatatgct ccagtgcgga atggctattg caagcaggtc
    17041 acttgcgact gttgcttctc cttcttccaa aaggacaagc ctgccatctg ccagcaactc
    17101 ctgcaatgct tcaccagcag gtgcagattc aagacgcgaa cgggcaacca tttctttcac
    17161 cgaagcagta tttagggcca gcgctgcttc aaacagaata tccgctgggg agccctgtgc
    17221 gagagattca agcgatttga gcacagtttc gtcaaagcgt ttgtgcctgc cctttggctg
    17281 atgatccaca atgacaccgc cgccaagggt ttcgctgggt gacggccgcc gcaggatgta
    17341 acggtcacca cgcacagcaa cgatggggtc acgcaattcg agttggatcc agccttcttc
    17401 actttcacga agttcttcgg cgccaagcag gcgcagagtg gcaatcgttt cgcttgcgcc
    17461 tgtgaagaat tttacttcgt cgttatgttt gatggatttg gagatgtctt tcaataaccg
    17521 cagccgcgca tcgattctac gggtgggcgt gtactggttt gggtgtgaga ccacctcacc
    17581 ccgctggatc tgctcagtgt tgaccccgga gatattcacc gccgtacgcg agccgggcag
    17641 agcctgctct tcttttttct tgtgagtttg caagccgcgc acacgaccct tctggcccga
    17701 gggcagaatt tctacttcat cgccgacggt gaggtgacca tcgctgaggg tacccgtgac
    17761 cactgtgccg aatccgctca tggtgaaaac acggtcaatg ggcaggcgtg gacggttaag
    17821 gtcggggcgg gcgggtttgt tttcgagcag ggtttgaagg ctggccgtta gttctttgag
    17881 ccccaatttg ttttttgctg aaacgtgcaa gatgggcgca tcttgcagga cggttccact
    17941 gacggcgccg cgaatgtctg tttcaacaag gtcaagccag gttgagtcag aaacgaggtc
    18001 tgtttttgtc aggacgatca atccagcggg aatttgcagc agatccagga tggcaagatg
    18061 ttccttcgtc tgcggcatga ccccttcatc ggcagcgatg accagcaggg cggcgtcaat
    18121 tccaccgatg cctgacagca tgttttcgat aaagtcgcgg tgaccgggca catcaacgat
    18181 cccaacttct tcgccgtttg gcaggttcat ccagccaaaa cccaactcga tggtcatttc
    18241 gcgggcttgc tcttctttga ggcggtcggg gtgggtgccg gtaagtgctg caatgagcgt
    18301 ggattttccg tggtctacat ggccagccgt tccaatgact ctcatgcctg tggttctttc
    18361 ttctttcccc tttctttgaa tgctgaaaga agaaagagga aagagttgtt ttattttttg
    18421 gattttttgc cgtgccccat gatgcgctga taggaaagca tccattcatg cagaacgttc
    18481 atcatttcct gtaattgctt ttctgtggca tcggtggttt ggtgcatttg gtcttgcaat
    18541 acctgggtga tgtcgtcgaa gggggtgatg cgggtttcga gtttttgcac ttccttgcga
    18601 atatcgcgga aagattcttc ggaggagagg ctataacctg tccagcgttt ttggtcgtcg
    18661 gtcttgaagc tgacccattc ctggcgcagg cggtcttcgg cgagacgctg catttcggtc
    18721 acttcgttga tgcgacgttc gagttttgtg ttgagctcga ggtaggtttc ctgcgctttt
    18781 tttgctccgc gcagggcttc gtccagattt tgaatgtgga tatcaaggtt ggcggcttct
    18841 ttttggaagg attcgtattt ctccttccat tccttccagg cgcggtcacg gtcgatctgt
    18901 gcgagggttt gttgttccag gaaggcggcc tgtgcctgct tgcgttcgag ttcggaggcg
    18961 atcaggtctg tgaaacgatt ttcaacattg cgaatgctat cggcggtgat ggtggctttg
    19021 tcgcgatttt catccacgcg tttgcgcagg gcggtcactt caccctgaat gtcagcgacg
    19081 cgcttcaggt cgttcttgcg ggtttcttcg atggccttta aagcatggac ggcgtcgtct
    19141 tttgtgcggg tggcttcatc cacacggaat ttcaaatcgg ctacattgtt caagactcgc
    19201 tgcatttcat cggcgcgttc tttgagttgt tttttgatct cggtcagatt ggtcgttgtt
    19261 ttgatgttcg caatgacctt ttggatctcg gtcaactcgg tttgcacata tttcagattt
    19321 tcttttgtag tgcgggtttg tgccttttcg ttctcttcga gcagttttac aaattccgcg
    19381 cgttgacggg tcatcagggt ttcaaactgg tcaaggcgct tctccatggg agagacatcg
    19441 gtgatctgtt tggtgaaccc tttcatttgt tttgagacgg cattgatggt cgtttcaaag
    19501 gactccagcc gcccgtcgag gcttgtcagg gcatcctgat ttttgcgctg ttgtttatcc
    19561 aaccattcaa ggcgtttaat gatttgttcg aattccattt tttatctctc cgatggctgt
    19621 ggggaaattt gcaaaattat agcacgcccg taaaacgggt tattgcccga ggtgttcaaa
    19681 cagggcgggc aggctggcga tgaagggttg cagggtctgc gggaaaattc cgaggatgaa
    19741 aagcccgatc accccgaccc cgagcatgcc catctgaacc caggattcgt ttatctccca
    19801 ggttcgattt tcttctgcca tgacaaaaac cgccagcgta cgcatggctc ccatcaacaa
    19861 gcccaacagc ccgatcaata cccagaacga catggtcaat gattcaggtg tcaattcctg
    19921 ccacagggcc gcacgggatg ggaaacctgc cagtagcgga aagcctgcca tcgaaagatg
    19981 ggccagaacg atggctgcga ctgcaatggg gtattggcgg gccataccgc gaacttcgct
    20041 gaagcgcagt gaatatactt ttcgttttat gatggaaagc gcaagtgccc aaaccgccaa
    20101 ctccaatccg cgtggggtga gcagcaagaa ggtgacattg acgacttcgg tggatttcaa
    20161 ccctgcagca aggaccagca gcccggtctc aacgatggag gcgtagccca tcatccttcc
    20221 gatatgcttt tgctgtgaag cgaaaagtcc gccgcttgcc accattaaca cgccggcaaa
    20281 ttgaatggca tttgaaagct gaggtgtgct tcgcagccag gtgtagcgat ctaaaaagtt
    20341 tagcaaaaat atgatggtga aggttggaag agcccacaaa aggaaacctg tggcataggg
    20401 tgaggcttct tcagtcagca tcggtatcca gctgtatagc gggaagatcg ccaatagaaa
    20461 ggcgaatccc aaactgagca tggtacctgc ctggatggtg ttgccaatgt caccggggct
    20521 ggcttccaca cctgcgagca tccaacctga aaacaagaca aacggcattg caagcgtttg
    20581 ctggatcaag aaccgaacaa tcccctgacc tggtctttgg tgcagtggaa ccaacagtgg
    20641 aatcaccaac atggctgcca tgtcgatcag cagtgctgca aacaggaatg gctccactgc
    20701 aatggatgcg gtcagcaggg ctacgatcat cagcccgagt gaaacaaagc ggttggctgt
    20761 gccagaggcc tgtgtgccga agaaccacaa ggtcgccagg ccataaataa tggccagcaa
    20821 tggaccatct gcagtgttga gcgcaaagct gcgcccgaaa aactgaaccg tggaagaaat
    20881 tttgatggaa acagagccaa ttagtaaagc ggtatcaatg ggaaagatca aggcggtcaa
    20941 tgccagcagc atggatacca ccccgccaac aaaggaggaa atacgctcct gtaacaggaa
    21001 aagaatcaaa actcctgcaa caaatgggat gaggatccaa atgatcggcg cgctcattct
    21061 tcatcctcca cttccagcgg aatggaacct gcgatcaaca agtaggaacc tgcaattccc
    21121 aaccccaaat tggtcaatgc cagcaaacca gccaccaaaa ttgaactttc cacggcggca
    21181 tagagtacct cgaaacctgt cagcatggtc agcaaaccca gggtgactcg aagaatatcc
    21241 gaggtaacac cgaggtggat cacacctgct ccgatcagca agatactgcc tgcgattaca
    21301 ggcaggccga ggcctggaat gctcgtttcg atgcgcggtg ttgcaccagc cgtgaccaaa
    21361 gccacgatcc cgatcaaaat gccgcggaac caccgtccgc tttgcagaga cgtatcttcg
    21421 gctttttcct ctgtggctgt caacccaaga cgggtcatgc cgatggccgc gaccaccatc
    21481 cagcctgtga tgagtttggc agagcccatt acaaagggca gatgtcgagt caccagccag
    21541 aaagctgcca ggtattgcaa agccagtgcg cccaacggga tacgccagtc acggctgatt
    21601 aagataattg cagaggttgc gagaatcatt cccactgcga tccatgaaat gatatcggtt
    21661 gccatgctag gggagcccct gtgtcagaag tgagatgaaa aggaccagga agagcagggt
    21721 ccacatcacg ccgccgttgc cttccagtgc ggtcgtgatc gtgagattga ttctttcaaa
    21781 gacgcggtat accgcccaga aactgctgta gaagacgctg ggggtatttg cagcaatggg
    21841 gttcacccaa tgagcacgga tcgggttgag gatgcgaaaa cgcggtgtcg cccaaaccag
    21901 cccgaggatt aagagcgaag cgatcaccgc cagcagccag gctccgatct ggcgcgcacc
    21961 gtcccagccg atcaatccca gaataatctg aagcactgca ggcaggatga tccccgctgg
    22021 ataaatacgg ttgacccaat tctcctgaga gtcgagagat cctctgtcac cggggcgcaa
    22081 tgaatggcga atgaggcctg cggtcaacaa ggcttgtgcc gcaactccaa acggaacgaa
    22141 gaagtttaaa ttgccaagcc agacactggc cgtaagtgaa aacggaagcg atgaaagact
    22201 ccatgctcca accagcagaa aacgactcag ccatttgtgt tgaaccgaag aaaggaacaa
    22261 agccccgccg accaaaatca gtgcgcatcc ccacgcaatt gccccaagtg gattgccatt
    22321 caaggttgct gtaattgaaa gcgcggcgat gccgatgatc caatatggac ggcctgttaa
    22381 ttcgtcgggc gcacgcagcc acatccaacc gccatagaca gcagcaatga gggcaaataa
    22441 aagtaaaaac ggtgtgacgc ttgaatttag actgccggca ggcacatggg ccagcaatat
    22501 caaactcgat gcggcggcaa ccaaacgcaa agcggttcca aacccgcgcc tcaacacgga
    22561 ttcagatgag taaggcaggt gcaagggaaa aacccccaac cgcagcccgg cggcagccac
    22621 aagatacaaa ccagaatctg cggcgatgga atgaaaatcg aatgtgcttc cctgtgcaat
    22681 actgatcaca tttgcccaca gcagcagaaa tgtgccaaat gcccgtgttg aaaaagaaat
    22741 aaccacccgt tcattgtttg aaggcccatc cactgaacga agctgggtga tgagttcggc
    22801 gaggtcaagt gcgctccaga tcagcagcaa agtgaacgga ttgtttgcag taacagccag
    22861 aattcccaaa ccgcccagcg caagcacacc tgcccacgca tacaaactgg tggaattggg
    22921 ccgcgttacc tcagtcagca ggatggtaag ggtgatgcca gagatagcca ccgcataagg
    22981 ccatgagatc gcatccgccc gaaacaggat cgggttttgg aacaaggtca atggctgcca
    23041 ggcgggcaac gtgaaatcca ccgggatgcg cgccagccag atgaacacac tggccaatga
    23101 aagcattgcc ccgccgatcg ccacaagcca ggtgtaccgc gcgtttggct gggtcacacc
    23161 taaaacaagc aggatcagtg cggttaaaaa aagcaacaag caggaaatta agattagcat
    23221 ggagtgaaca attgtatcag ttttattgtg gacgtacttt ccctgtttcg catttggtac
    23281 aatcataaac atgattcttc aaaggcggat tctcgctctg ctggcatttt ttctggcgac
    23341 ttcatgcgct tccactcccg accctctgcc tgcttctgtt cctttggccg ccccaacgct
    23401 gacacctttt caacccctgc cagaagcaac ttccactccc gatgttgccg ccatcccagc
    23461 cgaacccacg tacacccctc ttccccgaca gaatacagcc atcgaaatcc tgcccacacc
    23521 actattaata gacccatctg ccaacggcag tgctggtgat ttcagcttga tgaacatcaa
    23581 caaccccttg accgggctgc ccgtcaacga cccagccctg cttgaacgca ggccgatggt
    23641 gatcaaagta gccaacgcgc cagataccat ccgcccgcaa tccgggctga ccctggcaga
    23701 cgtggtttat gaatattaca tcgaatgggg tgatacacgt ttcattgcgg tgatgtatgg
    23761 aaacgactcg ccaaaagttg gcccggtgcg ttcaggaaga tattttgatg aacacgtcgc
    23821 ccgcatgtac aacgcctttt tggttttcaa gtttgccgac ccgcgcgaat attcctacct
    23881 gaaaaattcc actctgaatg agtttctggt tgtgccgggc ggttcagggt gcccgccatt
    23941 catctttggc cccgataaaa aacgggatga ttacaacaat ttcttttttg tcacttccga
    24001 atgggccgcc tgcgctgcca gcaaaggact cgataactct ccgcaagtca ttcgcggcgg
    24061 attcttctcc gatgccccca tggaaggcac aaccacagct tcacgcatct tttcctatta
    24121 ttcaaggcag agctacaact attgggaata tgatgtgatc acacacaaat atttccgcta
    24181 tcaggaagcc aacgatatgc tcaacggcaa acccgaagcc tacgcacccc tctctgatgc
    24241 cgagaccggt ttaccggtca atgcagagaa catcgttgtc atttttgtgc cgcatacctt
    24301 tgccaatgaa ttcaacgcaa aagatgaagt ttataacatt gacctgatcg attatggaaa
    24361 tgccttcgtg tttcgggatg gatttgttgt ccctgccaaa tgggagcgga cagatatcaa
    24421 ccagccgctt ttgcttacca atttggatgg cagtccgatc tacctgcgcc cgggccgtac
    24481 cttttacgaa gtgatgggtt ttacctcaac ctacacccag gatggcagtg actggcattt
    24541 caaatttgaa accccatgag aaaaagttta ccaatttgtg tgacaagcga taaaatacgc
    24601 tgcatccgtg ttcgtttgga gttgacatga cctttgaccc cgtattcctt ggaagcctca
    24661 cccttgtcct gaccgctttt ggcggcgcat ttcttgccgc gttgtggatc agtcttgtgg
    24721 tgtggacgta tcgggatatc cgtgcccgtg cccgcgaccc tttgacacaa acgctcgccg
    24781 ccttgttggt ggcagtcctc aacctgcccg gggtccttgt gtatttgatt ctgcgcccgc
    24841 cgcgcacttt ggaagaggaa tatcaacgca ccttggaaga agaagccctg ttgcaggcct
    24901 tggaagacct tccgctctgc cctggctgtg aacggcgcgt gaaagaagat tggcaggtct
    24961 gcccgaattg ccataccaaa ttgaaaaagt cctgtcacaa ctgtggaaaa tttatggaat
    25021 taccctggaa tatctgtcca ttttgcggca cacccgcccc ggggatgcgc cttgaatcca
    25081 caggcatgga tgatgcgttg cgcaacttaa aactgacgga tgactcaaca gaagtaaaag
    25141 tcgattctgc tggttgaagc ggccaatgag ggtgatctgt ttttggtaat agaataataa
    25201 aatcttcaag accacgaaat cagacgggtg tatcaaaaac ccaaaggttt cgtggtcttg
    25261 aagttatacc cgtcacggtc acaaatttag aagcgggaga gtgcaatttg tgaccgaggg
    25321 cattatataa aaataaagga ttccaaaatg aaaatagact cagctgtact tcatcactta
    25381 agcatgccgc ttgttgcccc ctttgaaacc tcttttggcc gcgaaacaga tcgcgaatgt
    25441 gtgttgatcg aactgcgttc tgaagggttg gtaggatatg gcgaatgtgt ggcctctcgt
    25501 gacccgggtt acaactacga gaccaccggc actgccattc atatcctcaa ggattttatt
    25561 ctgccgttga tcctcggtgt ggatgtgaag gacgccctcg attttcaaac gcgtgtggca
    25621 gggatccgtg gacatcacct tgcaaaagca ggcgtggaaa tggcgatctg ggatctgctt
    25681 gggaagcgct ctggcaaatc cctgcgcgag atgctgggag gagcgcgaga gaaagttgaa
    25741 gtgggagtct cgatcgggat tcaagaatca gcccaggggt tggtcaagac cgcggcggaa
    25801 ttcattgaac aggggtatgc ccgggtgaag atcaagatca aaccaggcag ggatgtggaa
    25861 gatgcggccg ccgttcgaaa agcattcccc gacctgcgcc tgcaagtgga tgccaattca
    25921 gcgtattcga tggatgatgc ccctctgttg aagccgctcg ataaaatgaa tttgctttta
    25981 atcgaacagc ccttgtttga agatgatatt tgggatcatc ataaattcca ggagcaattt
    26041 gagacgcccg tctgccttga tgaaagcatc gtttccccgc gtcacgcgcg ttacgccatt
    26101 gagatgaagg cgtgtcgtgt cattaatatc aaggcgggca ggctgggtgg gatcagtcag
    26161 ggggtgatgg ttcatgatat gtgcatggaa aaatccctgc ctgtttggtg cggcggtatg
    26221 ttggagacag gcattggccg tgcctccaac ctggcattgg cttccctgcc taattttgtg
    26281 ttgccaggcg atgtttctgc ttcagaccgc tattacaaac gcgatattac aaatgaacga
    26341 tttgtactga accccgactc cacgatcacg gtgcccaatg gccctggcct gggcatgacc
    26401 attgacgaag aagcgctcaa actgttttcg cttgcaacgc tttcaatcgt tgcaaattca
    26461 tgattaagga aactgtattg atattatttt gaagcgcaca gcgtttgctt catataatgc
    26521 gagacatgaa gatgcctcgc attatttttt tggaacgact tgaatcatat ctggcaggga
    26581 gatttacttt aattaaatcc tccttttttg tacgacaacc caactggaat ggacatcgcg
    26641 ccacacgcct tggttttctc ttgatcagca ttttgtcact ggttgcctgt cagccctctt
    26701 tgtggggggc tccaccgccg ccaccaacct ttgccgcagc aactccttcg gcgatgcccc
    26761 gcatcccaac tcttgcgcca accattaccc ccatgccgcc aaccgctgtt ccaaccccgg
    26821 tggttttgaa tgctgcccag gtttgggcaa gccctgccac accattggcc ttgcgtgaaa
    26881 aattacagca ctggggcttt caagtgttga ctacgaatca ggcctcggca aatttgcaga
    26941 tcgacgttgc ccaacccatc acaggtgcat ttgacacctc cacctggacc tatgccctcg
    27001 tggcaccgtt cccaaccatc attgatggac tttcatcaca ggaattgatc tcggtttgga
    27061 gtggcgcttc cttcggaccc tttgctggga ttccgatcct gatggaagaa tccaccatga
    27121 tgacctttac cgcgctgtgg ggcgcacctg cagcaggaag cgtacaagtt gtgcccgcag
    27181 accaattgct cgatacggcc tggaaccaga tgccatcgtg ggccatcatc ccatttgaaa
    27241 agatcgaacc caaatggaag gtactgacca ttgacggtca atctcccatt caaaagcatt
    27301 ttgatcccgt catttacccg ttgactgtaa cctatcggct gacctgtgct gagccttgcg
    27361 aagtgccagc cgaattggat ttctcctacc aaaaccgtga cccaaataaa ctcgccaccg
    27421 tcatcatgac cggtgtgacg gccatggtcc gtgcaacagc gaagacgatg gatgtgaaag
    27481 gcatcaccta ccccggagaa gagatccgcg acttgctgcg tgaagcggat gtaacccaca
    27541 tcaacaacga agtcccattt ttcacgggct gcccttcacc agacccaaat caggagaaac
    27601 agattttctg cagttcaccg cgctacatgg aattgttgac cgatatcggc acagatgtca
    27661 ttgagctttc aggcgatcac ttcgctgact atgaagatgt agccatgtat gaaacgctcg
    27721 atatctacaa acaatacaac ctgccctact atggcggcgg ttacaacgtg gaagatggcc
    27781 gcaaaccgct tctcatggaa gtgaacggca acaagatcat gttcatcgga tgtaattaca
    27841 aaaccattta cgctagcgcc accgataccg tgcccggtgc cgttccctgt gattttgatt
    27901 acatgacaga gcagatcgcc tactatcgct cgcagggata tttaccgatc tcaacttttc
    27961 aataccacga atttgatacc gccaaggccc gcccgcaaca gatcattgat tttcgcagaa
    28021 tggctgatgc cggggcgatc attgtcagtg gaagccaggc gcatgttcct caggaaatgg
    28081 aattctacaa cggcggattt atccattatg ggttgggtaa cctgttcttt gaccagatcg
    28141 ccaaacgcgg accgcgcctc acagagcgtg agtttattga ccgtcacatc ttttacgatg
    28201 gccgctacct tggcgtggaa ctgatcacag cctggctcac cgattacgcc cgtccgcgtt
    28261 atatgaccga gttggaacgt acccgttttc tcactgatat cttcaaggaa agtgagtggg
    28321 gctttttgca agtgggacag tagcaaacct cgaatggacg ctcctagcgt ccattctgct
    28381 ttttgtgtag aatgctcagc ctacccaacg tcattggaat ctgaatgaga aaaatccctg
    28441 tttacttact ttgccttgcc atgctcgcgg cttgtgtgcc agccacgaat gcgcctgccg
    28501 tagctacaaa cccaatacca actgcaacgg catctccaca acccgtctcc accaatacac
    28561 ccatgcctgc agcgttgtgg attgccccag cggtccctgc cgttttgcac gaagctgcaa
    28621 gatcttcagg gttgccaata accgaagacc ccgctctcgc cactcaaaaa ctggatatgt
    28681 ctgattctgg atctttgtgg atttacgctc tcgtcgcgcc ttttcccaca ctgacagatg
    28741 atgtggcctc acaagattta ctctccacct ggaaaggcgc atccagcgga ccgttgaacg
    28801 ggcacagcgt tctattggca gaatcaactc tgagggcgtt cgcttctgtt tggggcgaac
    28861 cagcttcagg ctcagtcaga agcgttcccg caggggagat ccttgataca gcatggaaag
    28921 agtccgcgtg ggcaatcatt ccttttgaag agattcaacc caaatggaaa gtgctcagcg
    28981 tggatggtca atctcctttg cgaaaaagtt tcgatgcaga agtttatccg ctaaaaattc
    29041 acttcagcct ttcacaacct gaagagtttg cgttgcctga aaccaatcgc gaccccgcca
    29101 aactcaccac agtgattttg accggtgtca ctgcgctggt acgtgccaca gcagtgacga
    29161 tggaattgcg tggcgtgacc ttccccggag aattcgtgcg tgacgaactg cgtgaggcag
    29221 atatcaccca catcagcaac gaagtcacct ttgacaagga ttgcccgacc ccaagctcag
    29281 gctatcgaaa ttttattttg tgcagtgacc cgaagtatat ggatctgctg ttggatgtgg
    29341 gtgtggatgt ggtggaggtg acaggcgatc acctgcgaga tcgtggtgaa gttgccttgt
    29401 tggatactct ggcaatgtac aaggccaata acctgcccta ttatggcggc ggcatcaatg
    29461 cagaggaagc ccgccagcct gtgttgatgg aagtcaatgg caataagatc gctttcatgg
    29521 gttgtaatgg aaaacgggaa gggaaatacc cgaaggccag tgcaaccagc cccgggccag
    29581 ccctatgtga ttttgatttt tttggcgatc aaatgcaggc tttgaaggcg cagggctaca
    29641 acatcatttt tacatttcag catgaagaat gttattcccc tggcccatgt tacacacatg
    29701 aagaaggttt ccgcagggcc gccgatgagg gtgcggtgat cgtcagcgga agtcaggctc
    29761 actttccgca tgtgatggaa tttcacggtg attcattcat ccattacggc ctcggcaaca
    29821 tgttcttcga tcagatgacc tataacctgc ccagcggcga agtcattgat gaaacacgac
    29881 gtgagttcca tgatcggcat gtgttttacg atggccgcta cctcggtgtt gaattgttaa
    29941 cctccatact ggaggattat gcacgtccac gcccgatgac cgcggcggaa cggctgcagt
    30001 ttctctccga ttacttttat tacagcggtt ggatgccttt gttgcccacc cctgttccac
    30061 aaccgacggt aacccttacg ccaattgcgc ttccttagta tatagttctt tgtgaaaaaa
    30121 tccatccgtt gccgcaagca taaatgcggt aacggatgtc taattttaag tgggtgtaaa
    30181 atagaaatat attcatcgtt gcgataacag aaacgcgaaa aatgcagaag atttttgcac
    30241 tacttgaaaa acggagcccg tacttttgga tattcacaag tctggtcatt gtgggactgt
    30301 taggcgttgt ggattatcta actgggaatg agtttagctt ttctttgttt tacttaattc
    30361 cagtctcttt gactgcctgg tatgtgagtt ccgctgctgg ggcaatattc tctattctgg
    30421 gcgctgtaac ctggctgatt gccgaccttg taattggtga aaattactca agcaaatttg
    30481 tttatttgtg gaacacgctc attcgttttg gtttttttgc cattgtgagc taccttatgt
    30541 ccgaattgca tcgagcgcaa aataagatgc aaactctggc gcgtactgat tttgttaccg
    30601 gggcttttaa ttcccgctat tttcatgaat tattaaaaac tgagcttgat cgttcgagcc
    30661 gatataaaag accgttcaca ctggtgtatc tggatttgga taacttcaaa caggtcaatg
    30721 atcgtttcgg tcatgacgaa ggtgacaaac ttctcacctg tattgcccag gagttaaaac
    30781 aagcggtacg taaatctgat attgtggcga ggctgggtgg agatgaattt gccgtnnnnn
    30841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaagcg gtacgtaaat ctgatattgt
    30961 ggcgaggctg ggtggagatg aatttgccgt tctgtttcct gaagcggggc ggcaggaggc
    31021 cgaagcgatc atggccaaag ttcatcgcca tattgctgaa caattgaaac aaacccattc
    31081 ttacgtcacc ttcagcgcag gtgcggtgac gtttgagtct tcaccaattt cacctgcgga
    31141 aacccttaga atcgcagaca aattgatgta tgcggttaaa aacagcacga aaaacggaat
    31201 ccaattttca acgtactctg ggtgagtttt actggggaat tggcaatgta tagccaccgt
    31261 caatgacaat ggtttgtcca acgatcatcg ctgcggctgg ggtgcagagg aacgcggtca
    31321 cttcggcaac ttcttcgggg gtgatcaacc ttccagctgg tacctgtgca atgaagtttt
    31381 gcaggatatt gtcctgattc cccatggatg cgaaatgatg cagcgcatcg gtctcaacga
    31441 cgcctggtga cacggcattg acattgattc cctttgagat caactccaca cccaggtagc
    31501 gcgtaagcga ctccagtgct gctttgctgg cgccaacgac gacataatcg ggcagtaccc
    31561 gtgttgcccc agcacttgaa atgctgacga tctttccgcc gccgcggatt tccatcaagg
    31621 ctgcggcgcg ttgtgctgca aataacaagg cacgcgcgtt gatgttcatg gtccagtccc
    31681 agccttttgg cttttgctcc agggcagggc ggttatatcc cgaagctgca ttgtgaatga
    31741 gaatatccaa cccgccaaac cccgcttgaa tctcatcaaa catgcggttg agatcgtcca
    31801 ggtcgcctac atctgctttt ataagcaggg cgcgccgacc gagtttttca acttcacgtg
    31861 ctgtttcttc agcgggggtg cgattgcgaa agaaattaac cacgatatct gcgccgtgtt
    31921 tggcgaagtg cagggcaatg gcgcggccaa ttccacggcc agagccggtg acaagtgcga
    31981 ttttgttttg aaacatgatg gtgtactcgt tttctgtgta gatactgcct attatacgtt
    32041 gcagcgctat ttcaggtacg agtcgaaaaa tttgatcgtc cgctccatgg caatggagaa
    32101 attggccgag atattgtggt tatcaccttc gtagatgtat aaatccgcag tttgacttgc
    32161 attgcgtgcc atttctgcaa ggcgaatgga aaaatccaaa gggacgtctt catctgcggt
    32221 tccatggtgc aattccaacg gtccagacag atcgctgaga tacgatgtcg ccgatatcga
    32281 atcccaaaat acaggattct gttcaggagt gccaaattcc tcgatccatc ttgtgcgcca
    32341 gcccacccca cgtgaacttg gtgagggagt gaatgagcct gtgcgccgcc agttgtacag
    32401 caggtcttga taagaggcca ccacacccgc ccagatcaca ccgaccttta catcttgcga
    32461 gatgaccatg gcacggagtg tgaggtatcc gcccatggaa tgtccccaca tgccgatctt
    32521 atcagggttg acttcaggca gctgtttgat ggctgcaacc gcatttaata catcgatttg
    32581 atacccggga tcgccatacg ctccagtagg ttcaccttcc gaggcgtcat gaccacggta
    32641 gtcaatgcga aaaacaatgt aacctgcttt tgcgatttca tccacgtagg caatataacg
    32701 ctcagtggtg cgatacacat cgggtggaat gtatccatga ttaaagacga tggctggcca
    32761 gccgccttcg ggtggttcgc cgtttgggat ggttagcagg gcatagattt ttaaaccctc
    32821 tgaaaggtag taggaataat agcggcgata gttcgaaccg cgatccagtt ctttgacaat
    32881 ggtgatttcg ctgccagggt attctccatt ccgcatggcg atgatgctca tcgggtatgg
    32941 aattggagtg gcggtgggga tcggtgtaaa cgtggatgcg aaagttggtg aagcagtttg
    33001 tgatggggga atggtttgtg tagcggtggc aacaggggtt ggcggtgaaa tcgctggtga
    33061 ttgtgtgctg caagcaataa gaagaaggac cagtgaatac agataaagtt ttttactcat
    33121 tctgaggact cctgtcctca agatgaggtg aagcagattt ttctaacctg gaaccagcat
    33181 ttcatctgcc cacacagtaa cgcggttcct gcccaagcgt ttggatttgt ataaagcgcg
    33241 atctgcctga gttagcagag agtcgaagct taaggaatat gcattggagt aagtggatac
    33301 cccaagactg atggtgattt gaacaagctg accatctact ggcagtttgg tgctttccac
    33361 tctttcgcgg attctttctg cgatcactcg cgccgtctct gaatctgttt ctggcatgag
    33421 gatgacgaat tcttcaccgc cgtaacgtgc aaagacatcg gtcttgcgaa tggaattgtg
    33481 acagatctca accaacttta tcagcacgag gtcaccgatc tgatgcccga acgtgtcgtt
    33541 tacgcgtttg tagtggtcgg catcgatcac gatgaccgaa agcggtttcc catcttgttg
    33601 ttgtgcccgc tccatttcga tctttgccag tttgtagaac tgcctgcgat tgtatgcttg
    33661 tgtcagcggg tctgtgatcg ccatttcttc aaggatcttg gcctgctctt gcagttcagc
    33721 ggtgcgctca ttgaccgttt tttccaaccc gtttgcaagt tcacgcaatt cactctggcg
    33781 ctgttggatc gattctgcca tttggttgaa agccgtggaa agttggccga tctcatcatt
    33841 taccctcggt tgattcgccc gaaccgtgag atcgcctgcc ttcattttgt tagccacccg
    33901 tgccaactca cttaatggag atgtgatctg tcgggctgtg ccaaatgtga acaggccgcc
    33961 gaggaaaatc acaaccagtg caagtgtcag cccttgcaaa gtcagatctt ttactttttg
    34021 atcatgcttt tccgtggaaa aaccgattgc aatggctcca atggtctggt ttccgatccg
    34081 aatgcttttt cctgaaacca gttgagttcc ctgccatgaa atatatagtt catgggtggg
    34141 ggtagtgacc aggttgtaac caagcacgtc cacttcttgg gaaaattcca actcaggttt
    34201 ggatgaatcc accagaattc tccccgtttt gtcatacacc acgaacattg tgatctgcgg
    34261 gttttccccg attttcccag caaggtcttt gagttcgtca atttcaaggt tgtagagcgg
    34321 gtcacgcaag gtgagtgaga tcgtgtccag caacagcccg gcttgcgaga tcatttcctt
    34381 ttgtgaatat tcccattcgc gttttgtgta ttgataggtc aataggagaa ccgccagcac
    34441 ggcaaggaaa ctcatacggg ttgctatttg tcttgcaaga gatggcctca accaaaacgg
    34501 agttctcata taacaggaag tgtatcagat tgcgctcttg ggcgagaata caatattgaa
    34561 aagagactca caaggtctga aagcttgtga gtctccatgt ttgaggccgt atttattgct
    34621 ttgcggctgc ttctgcttcc cgtagttttt cagtcgcacg gaagtatttc aagtagcgca
    34681 ttgagtttgc atcgcgcccc aggagcaggt cagtggcttt gatcgtgctt cccagcttga
    34741 tcgggtcagt gatcgagcag gtcacacccg tctgcatggc gagtgcaaga aatgccgcat
    34801 tcaccgaatg cctgtcgggc aacccgaagg aaacattgct tgcgccaagg ttgatgttga
    34861 cgccaaattc cttcaccagc aattcgatcg tcttgagtgt aacggtcgcc gcttttgagt
    34921 cgtgaccgac tgtcatcacc aggggatcaa taatgatatc ttcgatcgga atgccgatct
    34981 tggctgcgcg ctcaatgatt tttgccgccg ccgcgagacg taactctgct gtgggcggga
    35041 tgccatcatc gccaatggtc agcccgatga cggctgcgcc tctatcttta acgatgggca
    35101 gaacagtttc aagctgcttt tcctccccgt tgacagagtt taccaacggc ttaccgggag
    35161 ctaccttgag tcccgcttcg aggatctttg ggtcattcga atcgatgcac aacggcacat
    35221 ccaccaccga tttgaccgct tccaccacca ttgggatgat cgcagcttcg tcgatctggg
    35281 gatggccgac atttacatcc aacacatccg cgccccactc cacctgacgt tttgcaagct
    35341 gtttgacgta atccatattt ttgtcaacca atgcctgccc gagttttttg atgcccgtcg
    35401 ggttgatctt ttcgccgatc atcacgaacg gcttgtcaat accgatgaca acttccttct
    35461 tttctgattt tagtatggtg tgcatgtgct ctccaaattg agttttattc cttcaaaaat
    35521 ctcagcgtcg tttccaccaa cactgctgtg ccgattggaa gcgcgcgttc atccaggtca
    35581 aacttgggat gatgcagttc gtaaacctct cgtcctgcga tcatcgtacc aagagtatac
    35641 atggctccgg gggcatcccg caggaattcg gggaaatcct ccgcgcccag cgatttatgc
    35701 atcgggtgaa cattctctgc tccgagcagg tctttaccga cctgatcgat gaccttggaa
    35761 acatgcccat cgttgatcat gggcaggccg ccaaagagaa aatccaactc gtaatcgccg
    35821 ccaagggtct tggtgatttc aaagacgcgc cgcatctcat cacgtaaaag cttctgcgtc
    35881 tcaagcgatg tgaatcgcaa agtgcctttc aattttatct ggtcagggat gacgttttcc
    35941 gcatcgccgc cgatgatcga gccaatgctc aatgccacag gctcaaacgg attcattctg
    36001 cgtgaagaga tgttattaag ccccatgata acatgcgcca gcagtacgaa cggatccact
    36061 gtggtgtgcg gatacgctcc atgcccgccc ttgccgttga tggtggcaaa ccatgtatcc
    36121 acaccgccgc cgctgggacc tgcttcaatg gcaatcgtgc cgacagggct ggtggggtct
    36181 acatgttgtg caatgacata atcaacccct ttggttgcac cttcttcgta catgcgttgt
    36241 gcgccgctct tgccttcttc gtccgcggtt tcctcggaag gttggaagag aaagcgcaca
    36301 cggccagtga atttttcttt tgccaaaatt tgcgccgccc ccagcgccat ggctgtatgt
    36361 gaatcatgac cgcaggcgtg cattttccct gtgttttgtg aaacgtagtc cgtttcattt
    36421 tgctcgaaga tgggaagtgc atccatgtct gcgcggatcg caaccagctt gccgccttca
    36481 ccgatatctg ccactacacc tgttttgcca acccctcttt gtacgcgata tcccattttc
    36541 tccaattcat ctgccacgat cccagccgtg cgatggacat cgaaacccgt ttcagggtgc
    36601 atgtgaaaat cgcgccgcca ttcgatcaat tcttcagaaa ttgcgtttgc ctgtttcaac
    36661 atatccattt tccttttgcg ctaaagtatc cgcttcttac ttctttttta ccatctcttt
    36721 ttcagccgcg gccataattc tatcaagttc cttctcttgt tccggttcca gcggaggaac
    36781 ctggtgctcg cgcagaatcc tttcggattc ctccattgca taatctgcca tttgctttct
    36841 ttccagcttg tcttcccatg tttcccagga attgcgttcg gcaagtttgg tcagggccag
    36901 ttcgccagcg cgcaggtgtt tgagtgtgtg tttttgaccg agataatttc gtgttccatc
    36961 catgacattg ccgatgattt caaccgccag atgctccaca tcggcagaaa ggcccgatcg
    37021 caggcgcatg acactgccga ccagttcatt gtccaatacc atttgcgcgt acgatcccat
    37081 cacgcctgct tccatttcgc cgataccgga aagttcatct gcaccagcca aagcagggat
    37141 tgcggcattt agtccgcgtt cgaaggcgtt ttgtgcatca agggtgtgtg cattcgttga
    37201 aaacccatac acgttgacgc tgaacccgta ataatgtccg agttgaaccg tacctgccga
    37261 agataggcca agttccacgc cgccccagat caatcccgtc cgcggctcca gcatgccaag
    37321 tcgtccgcaa tacaccacac cacaacccgg gttgacgatc tgcgcaagca cgacaagggc
    37381 aagcgtttcg gcgctttgct gaacaagact gcctgtgatg gtcattggtg aagtggctcc
    37441 gcctgtaggg gcggggagat tggagcagat gacgcctgct tttgccattt caacaatggc
    37501 ggcggcggca ggatcgtgaa tggtcaacgg actgacaggg gaaagcgaca gtgtcaattc
    37561 ttcaggaggt gtgccaacca cctgtgccat ttcgactgca tactttactt cttctgcaaa
    37621 ttgaattcca ggaccctgca ggggtttggt ggtgtatggc agcgaatgac gatacatgta
    37681 caaagacatc aattcgccag gcacatcctg cggtgtgaag aatggagtga ctgtgtggca
    37741 gtttggcagt gcatccaaaa cccgggtggc gtcaatcaca tcctgatcgg tggcaatttt
    37801 ccgtgtgcct gttctggcgt cgaacacatc gcgcgcgccg ccgagattgt ggaagtacag
    37861 gtttccgccg ccaagttcgt tggtaatacc attgcgtccg cgaatcttcg ggcgtttatt
    37921 ggcttttgcg atgcaatcca ttacaagttc gggcgacatg caaacccgat tcccttttat
    37981 ggtgcagcca gctccaagca ggatatcgag gcttggcttg tgagtccaga taagtccaac
    38041 ttcgctcatg atgcgcaatg tggcttcgtg cattgccttc acgtcgttct cgctaagaaa
    38101 cttcaacggc tccattctct ctcctcatta agatttattt tttgagcacc atctctaaaa
    38161 acgccgcgct cttttcttct gcatcgatca cgttctcgat attcaaaaaa gcatggcctt
    38221 catctttgta taacagcaat tccacttcct tgcccaattc cacaagttta tcgcgtgccg
    38281 cgatcgaatc cgacgcagga cagcgcggat cgtttccgcc gcagatcatc tgcacaggtg
    38341 cgttgatttt atccaaaaag aaatatggcg aatgcgaatg ccaaagttcc ttgttgtctt
    38401 cggggtcgcc catgttctca atgttccagt gctgcagatc ttcgcgtgaa tccttgtgcg
    38461 atttgaacca attcaagaac ggcaccaccg cagaacctcc cgcgaacaga tcaggatatc
    38521 gcgtcaggca ggacatggtc aaaaagccgc cgtggcttcg gcctgtgacc gtgatcttat
    38581 ttttttcagc caggccattt tcaaccagat acttcacgcc cgcggcacaa tcatctgtat
    38641 ccacgccacc catgtcgtag cggctggcgt tttgccactt tttgccataa cccgtcgagc
    38701 cgcgataatt tggcgtcagc accgtccatc cgcgcgaaac cagataactc gtcgtaggtg
    38761 accacaaaga ttgcacatgc caattcggcc cgccgtgaat atcgatcacc gcccgttcgc
    38821 tctgaccgcg atacaacaac gcgggcacct gcacaccatc catgcctgca taccaaactt
    38881 cctcaggctg aacaaattga acatccttca actcggcagg caatgaatcc gtcaactgct
    38941 tgcacgaacc atcttccaaa ttcaacgtcc acacatcgca cggacgactc gaatcttcat
    39001 acaggatgac aacgccctcg gcagtgaact tcggaaaaga atggacgccc gccccaactt
    39061 gaatctgcgc gacgtccgcg cttcctttct taaactggaa gctggtctgt gcgccttcag
    39121 aatgaaccca cccgacgcga agcgtgtctc cgctccgcga ccatgcaggc tgtgtatcat
    39181 ctccgacaga ttcgttcacc catgtgattt cctgagtctc cacattgaac aatccgatat
    39241 catgccattc aacgttttct ccacagaacg cgaggaactt tgaatcaggc gaccatgcgg
    39301 gatgctgtgc attcaacacc ctgccattga ccttcaactg caccgtgttg accttcggcc
    39361 ctttgcgggt gcgtcccaca ggcacaacat ggatcgacct gtcactcgcc gttgactcag
    39421 attcgatggc gatccactga ccatcaggcg accagaccgc atcccaacag gggtgaaaaa
    39481 tatttttcag caaacgcacg gggcttccgt caatggggag caggtacgcg gcaaattgtc
    39541 ccttcatgtc cgaaagcacc gccaacatct ttccatcagg tgaccaactg acatttggct
    39601 gatgcgcgta ggcaatagaa ggcgtcaggt cggtggtggt gttcgtggtg aaatcgtgga
    39661 ttgcaatgtg atacgactcg ctgccatcca ggtccagcgc gaaggcgagc atggaaccat
    39721 ctggcgagaa gcgcggagaa aattttgcgc cgtccaatcc aagcatcaat tccctggtcc
    39781 ctgattctcc agcctcccac aactcccact ttcctgattt attccacgaa aaaatcaacc
    39841 tctgtccatc aggagagaca tcgtagggga ggacattatc cacatggggg acgcggagca
    39901 attgagcaag attaagcatg aacctatttt tcgctttcga tttttaatat tttgccttca
    39961 acctaaaccc ttcaacctgc aacttccatg gcttccacca caggcaactg ctcaagacca
    40021 gcttgttggg cgaggttcaa aacataagcg tggagttctg ccactttggc aggatcaagt
    40081 tcagggcggc gataagaagc gaggatttca tccacacgag tccgcgcccg ggagaaggta
    40141 tcctgtgagc cagattcctt ccagccgcgc atcgagtcgc ggtcgatcac tttgctgggc
    40201 atgtgttgct cttctttgaa gagtttcatc gtgatttttt gtttgaggaa atcgccgcct
    40261 ttgaagttca ctccttcata aaagccagtg gcgagcgttt cggtttgaat cttcatgccc
    40321 tgcaccatgc gctttgccat tgcaatgcct tctgcgtcga tgaccagttt ttcagggctt
    40381 tgacataaaa gcgaatcgac catgcctgaa ccagagatca tgttgatgcc gctcaatgcg
    40441 ccgatcatcg cacccatgcc gctttcaagc cctgcctgca tatcgaggat cttggattcg
    40501 gtggcgccga ggtaggcatg agtcggcaat cccaaagtct tgccgacctg cgcatacgat
    40561 gaatcgatca tcgcggtttc aacagagcca aagggcgtgc cgcctttgcg catgtcaaac
    40621 gtggatggcg ctccgcccca aacgatgggc gaacctgcac aagcaagctg gtgaatggtg
    40681 atgcccgaga gacattccgc ggcgtgctgg gtgactgcgc ccaacaatgt gacaggagcc
    40741 gccgcgcccg ccaatggcat ggatacgatc tcagcaggca cacccgcgcg cgccagcgca
    40801 atgagattcc ctgcgccaaa gtttgaccag atcaacggcg gcgatgggca ggcatcgaat
    40861 acggcacgcg gatacttgcg taaattttca tggctgcccg caaagatggc aagcatttcg
    40921 atcatctgat gcacggtctt gttggtgaac gcgcccgtga cgatgggttt ctttgaatac
    40981 atcatgacca gatataaacg gtacaggtca tgaatatctt tggggacgtc gtgacaaacg
    41041 atcgcggtgg attgtgcgtc gtactgcggc aggctttccg cgaccttgat cacacggatc
    41101 aagtcggcgg tttcggctgt tttgtgttcg agggtttcag gaatcaggat cgagatcccc
    41161 gaggagcctg ggtcgaattg aacggcatcg ccgccgtatt gaacggcggg gttgccgtca
    41221 tagtcataaa ggaaaaactg gcggggaacg gtttgcaggg ccgttctgac gatctgctct
    41281 ggaatgcgga cgaccatggt ttcttcatcc acttgtgcgc ctgcatccgc gagcaattgc
    41341 ctcgcttcag ggttttgcac tttgatacca ggcttgagca tcaattgata ggcttcttcg
    41401 atgatgcgtt caatgagttg atcgtcgagc agggatagtt tgggttgcat ggatactcct
    41461 tgtgtgtaat tggagattgg taaatggcaa tcaggtctaa cgacctttga atcgtaaaac
    41521 caatttcacc ttgcgacagg ctcttgatta ccaatcgcca attacgaatt acaagaagtt
    41581 caggcaggct ggaaatcttc gagcttgggc aattcttcga gaccgcactt ttgggcgagg
    41641 tccaatataa aggcgtggag tctgctttct ttgacaggat caatttcagg tcggcggtag
    41701 gatgcgagca attcgttcac gcggagtttt gcgcggccgt aggcatccag gctgccagcg
    41761 gctttccatg cgcgggttga atcgcggtcg atcacgctgc tgggcaggtg ctgttccttt
    41821 tgaaagagtt gcatggtggc tttttgtttg aggaaatcgc cgcctttgaa gttgatcttg
    41881 tcgtcgtaga aacctgttgc aagcgtgtcg gtgtgttgtt tgacgccgcc gatcatgcgc
    41941 tttgccatgg cgatgccttc cgcatccatg acgagttttt cggcgctgtg acaggcgagg
    42001 aagtccaaca tgcctgagcc agagatcatg ttgattccgc ttaatgcgcc gaccatggcg
    42061 gagatgccac tttcaagccc ggcctgtgcg tcaacgagtt tcgaatcggt tgcgccgaga
    42121 tatgtgtgcg tgggcatgtt gaatgtctta acgacttgca cgtatgaaca atcaagcatg
    42181 gcagtttcga ccgcgcccat cggtgttgcg cctttgcgca tgtcgaagat cgccgccgcg
    42241 ccgccccaaa cgatgggcga gcctgcgtgt gcaagttggt ggatgacgat ccccgcaaag
    42301 cattccgccg tgtgctgggt gacggtgcca agcatggtca caggggcggc tgctcctgcc
    42361 aacgggacag agacgatctc ggctgggatg cctgcgcgtg caagcgcgat cagattccct
    42421 gcgccgaagt tcgaccagat caatggcggc gcgggacaga catcgaagat ggcgcgcggt
    42481 ttttcacggg ccgcttcctt gctgcccgca aacagcgcga gcatgtcgat catttcctgt
    42541 acggtcttgt tggtgaatgc gcctgtgacg atgggcttct ttgaatacaa cagcgtgagg
    42601 tacagacggt atgaatcttg aatgtctttg ggaacgtcgt ggcaaatgac cgcagtggat
    42661 tgtgcgtcgt attgtgggat ctgttctgcg accttcaaca ggcggatcag gtgttctgtc
    42721 tctgtggtgt cgtgctcaag tgtttcaggg ttgagcatgg taatgcctga cgaacccggg
    42781 tcaaagtgaa ccgagtcacc gccgtaatgc acggtgggat tcccatcgta atcgtacaga
    42841 tagaattcgc gcggtacgct ttcgagtgcc ttcttgacaa gcgcctcggg aatcccgacg
    42901 acattattat cgggatacat gatcgcgcct gccgatgcga gcaaatcccg tgcttcaggg
    42961 ttttgtacct tgacgcccgg acatcgcaat aattcgtagg cctcttccaa aatacgggcg
    43021 atgatttcat cgctgagcag ggtgagtttt ggctgcatga gacacctcta atgagttgga
    43081 atgttggaaa gttggaaggt tacaagttgt caacctgtta acttgtgaac cttccaactt
    43141 gttaactctt tatttctcta ccgatttgat gattctttcc agttcctgac tcaatctttc
    43201 gtccagcatg tcaggctggt gttccttcaa ggtcttgcgg gcttttgcca gtgcccagtc
    43261 acgcgcatcg tcttttttcg tttcccattc ggagtaggga cggcggtcca tgaactgggg
    43321 catgaacaat tcacgcatgt gtttgcgggt gtgcttttgc gcgaggtaat gtccgccggg
    43381 gccgacattg gcaatcgatt ccatcgccag ggtctcgtca ttgacttcga ttccctgcat
    43441 catcttgtgg acgatggaga agatctcgca gtccatcatc atctcggcaa aagaccagat
    43501 gcggctgccg tgcaggaatc cgcacccaag cagcatgtcg gacatgacca cgcttgccat
    43561 gaaactggac atggcgcctt ccaagcctgc ctgccagttc ggttcttttg cgccagtggc
    43621 aaacgcaccc attgaaagcg gaatgttgta aaaatctgcg aggacgttgg tcgctgcacc
    43681 gaaaaggaaa tcttcaggtc cgccgcctgt gtatgcgcca gtctttagat cagaagcggt
    43741 ttgcgcagcg gcataaaaaa cgggggcacc gggatgggca atttgcaaaa gcgcggtggc
    43801 ggcaaccact tctgcattgc cgactgcaag attgccagcc atggtggtgg ggcctgttgt
    43861 ggcacatgcc gccatggtca taaagcctgt tggaattcca acttcagcag cgaggagtgc
    43921 cgcatcgagc gatccgccgt catgtccaag cgggggagcg gtgcattgca tgagcgagag
    43981 aattggccgc tcgcggagtt tatccctgcc gccaacgatc gccgcagaca tctcaattgc
    44041 ggctttggcc tcctccacgt tgtaaatgga ttcggtctgc acgtgtttgg tggagttttc
    44101 ccaaacggct ttcaattcgt gcaatccgcg tgcttcgacg ggggcatcct gcgccgagac
    44161 tgcaacccag tggaatgcaa cttcttcggt tgcatccgct acacgggcga tgtcacgtac
    44221 atcttgcagg caggaggtgc gtttctctcc tgtgtggatg tcgatgactt ccacgccgca
    44281 gccatcggtt ccgaggttga catggtttcc gtctagttgc agatcctgct gtgggtcgcg
    44341 tgccgcaagt ttgtaagcag gcggcgcttt cttgagcgca tcttcgatga cctgcggctt
    44401 gacccgcacg atcttctttt cgtgatccac ttccgcaccg acagattccc agatctccag
    44461 cgcgcgtttg gatggaaacc gtacgccaac cttctcgatg atctgcaatg tggcgttatg
    44521 gatttttagg acgtcttcct tggtcagcac ttccagtttc aacttggggt tgctgatggt
    44581 tttgatattc ttttgagaca taagccctcc gaggcaaaat gtctctcttc tctctccgag
    44641 ttaaaccttc aacttgtaac ctgtaacttg caactctttc ccttacgcgt ctgccttgcc
    44701 aaccagttct tttgcaatct tcactgcgcc gatggcatct tcagagtagc catctgcctt
    44761 gatcttggtt acccagtcac gggtgatggg agcgccgcca accatcactt tgatgcgagg
    44821 tcgcaggcct tctttatcca actcttcgat cacttcgcgc tggcggacca tggtggtggt
    44881 gagcaacgcg ctcatgccga tgatctgggc gtcgatctcc agtgctttgc cgatgatctt
    44941 gtcagcgggt tgatccacac cgaggtcaac tacttcgaaa ccagaggcgc tcagcatggt
    45001 gccgacgaga gtcttgccga tctcatgaat atcgccttca acggtggcaa gaacgacctt
    45061 gcccatcatt tcacgggcgg agccgagttt gagcagttcg ggttccagaa cggccacggc
    45121 tgctttcatt gcttcaccag ccatcaccag ttcaggcagg aacgcttcgc cttttccgaa
    45181 ttgatcgcca atgtagttaa cgcccactac aaagccgttc gtgatcgcgt cgagcggatg
    45241 catgttcagc tcgatcgcct ttttggccag ttcggtggag atatcggaat ccccatcaat
    45301 gattgattgt gccatttctt tgtaaagttc ttcagacata ttcaagcctc cttattttgg
    45361 ttttttcaac aaatgagtga tttgcttttc tttttctttc aataactttg caggtatgtt
    45421 taaatgagtt tccagccacc tgccagattc catcacatgt tccaacgaaa ctttgaccgc
    45481 aaggtctgcg tcgtgttttt ttaacgcatc aaggattgct gcatgttcat catgggtctg
    45541 tcggacgctt tcatccacca gctcgggttt gcgaaacagc gcctcgtaca atagccagtc
    45601 aggaaatgcg tttgacacaa tagtataaac ctgaaccatc aatgcgtttc cagaagcttc
    45661 ggcaattgcc gcatggaatt cccgactgag ctgcctttcc tgaggcattt cactcaatat
    45721 gacatgcctg tccatttctt ccatgatcgt ttcgatttgt gtaagttgct gaggcgagat
    45781 attctccgca gcttcacgtg ctatcaatgc ttcaagcatg aggcgcagcc cgtatgcctc
    45841 caccacttct ctggcagata tctcccgcac cctgacgccg cggtagggaa cacgctcggc
    45901 aattcctttt gccaccaaca agtcgaaggc ttcacggact ggggtcatac tgacgcccat
    45961 ttggctggca atatcttcct gacgcagcca gtcgccaggc gtgtatttgc cggcaatgat
    46021 ctgcctgtga agcgcatcgt aaatttcctc tttaagaggt ttgtgggtga gttctttttc
    46081 catggcttgt caatatgatt atatattata tataattagg gtgaatttcg gaagtaagaa
    46141 ttgtcacgaa aattgtcaca tgaaagccag gatcagtttg aaaataaaaa tcgtgggcca
    46201 gcaataaaaa attcgtttaa gttatactcg gagtactgga gcacatatgt ctgacattga
    46261 tctgaatccg ctaaaacctg caattgaatc ttatattccc ctcggccgtt caggcttgtt
    46321 gcctgcgttg catgcggcac aaaagattta tggatggctg cctgaagagg ttgcgtctga
    46381 agttgcaaaa tcgctgcgtg tgccgctggc agatgtgcat ggcgtgatcg aattttattc
    46441 gctctattat aacgagcagg tggggcagaa gtttattcgg gtttgtactg acccagcctg
    46501 tgcattaaaa ggtgcagacg gactcctcaa gcacctgtgc aaaaactaca aagtggatgc
    46561 gggtcaaacg accgatgacc tgtctctgac cattgaacaa agtccatgcc ttgggctttg
    46621 cgaccaggcc cccgctgcac ttgtggatga tgacgccgaa acgaatattt cgcacaattt
    46681 ccgttcctat gatattggcg tgccgcaatc actggtctat ggctcgatcc gtgagttgac
    46741 cgcaaactgc ggggaaggca caaccaccct tgcgaaatat ggtgagtatt ccgcctacaa
    46801 gaaagcatta agcgtcaaac cagaagatgt cattgctgaa gtaaaggcca gtggattggt
    46861 cggtcgtggc ggcgcggcat tcccaacagg tatcaaatgg gaaggcgcgg ccaaagccag
    46921 cgctgatcaa aaatatgtca tttgcaacgc agacgaatcc gagccaggga ctttcaaaga
    46981 ccgtgtttta ctgttggatg atccgcaccg taccatcgaa gggatgtgca ttgccgcata
    47041 tgcgatcggt gctacaaaag gttacatcta cattcgtggt gagtacccgt atattgtccc
    47101 tgttctcgaa gaggcgttga acgaagcaag aatggcgggc tatcttggag aaagatttga
    47161 tattgaaatc cgcgttggcg ctggtgccta catttgtggc gaggagaccg cgctgtttga
    47221 atcgatcgaa gggaagcgcg gttttccgcg tgtgaagccg cccttcccga cgacgcatgg
    47281 cgtgtttggc aagccgactg tcattaacaa tgtcgagacc atgtgcaacg tgccgctcat
    47341 cattgaaaaa ggctctgccg aataccgaaa gatcggcacg gaaaaatcac ctggcccgaa
    47401 attgttctgt gtttctggcg atgtttccaa accaggtgtg tacgaagtcc cctttggtgt
    47461 gactttgcgc gagctgcttg aaatggcagg cggtgtgaca ggcaaaaaag cattgcaatc
    47521 ggtgttgttt ggcggggcgg ctggcgcttt tgccacatcc gaacatttgg acgtgagaat
    47581 gacctttgaa gatctgcgcg ccgctggctt gccgcttggc tcgggcgtgg tcatggtttt
    47641 cgatgaaacc cgcgacatgc gcgatgtttt acagcgactt ggtcatttct ttgctcatga
    47701 atcctgcggc aagtgctacc cctgccagat gggtacacag cgtcaaatgg agattttgaa
    47761 tcgtgtggca gcggggaaaa ccctgcccga tgacatgatc cgcttgcagg atgtaggctg
    47821 gacgatgacg gaggcaagtc tgtgtggttt gggacagacg gctgcaagtg cagtgttgtc
    47881 tgccatgaag ttatggcctg cactttttga aaatgggggt ggaaaaacaa agagtgacaa
    47941 gaagtctgta gccgcaaagc cgcgtgcagc ctctgcgagg aaatcagtaa aagcaaaagc
    48001 ggtcaagaaa accacagtaa agaaacctgt cgcgaagaaa acggtgaaga aggctgttaa
    48061 accttccaag ccgaaggcga agaaaactgc tacgaaaaaa tccaccgcga ataaaaccgc
    48121 gaagaaacgc taaaggttaa gccatgactg aaaccgtaaa tctaattatt gatggacagg
    48181 aagtcagcgc tgagcaaggt agaaccctgc ttgaagtagc ccgtgcaatg ggaaagaata
    48241 tcccaaccat ctgttttcat gatgcgacga cagccaacgc cctttgtaga atctgtgtcg
    48301 ttgaggtgga ggggcagaga gtgctccagc cagcatgcat cgtcaaagca ggagcaggca
    48361 tgaaggtcca gacgcgaagc gagaaagtca tccgcgcgcg aaggacgatt ttggaaatgc
    48421 ttgcctctac catggacctt tccgaggcgg cggatatcca gcagatgatg gttgagtatg
    48481 gtgcttcatc ggagcgtttt cccgatgctg aacggcgtga gtcggaggtc aaggacgata
    48541 acccgatgta cgtccgcgat tactcgaagt gcttgctgtg ttggcgctgt gtgcaggtct
    48601 gtgcggaaga tgcgcaatat acttttgcga tcaatttcga tgggcgtgga tttgaaaccc
    48661 agatcggcac gttctttgac aaagccattc cgcagacatc ctgcgtgatg tgcggacaat
    48721 gcgtgggtgt gtgcccaaca ggcgcattga aaccgaaacg cgaattttta ttggaacaag
    48781 gcatgagccc gagcgagatc agtgtgatga acacgggccg caagcaaagg aagccgcgat
    48841 gattaaatct gaccgagtta ccacaacaac ctgtccctat tgcggagtgg gatgcacttt
    48901 gcaattgcac gtcaaagacg attttattta caaggtgacc agcccctttg attcaccggt
    48961 caatcagggg aatttgtgcg tgaaaggccg ctttggctac gattttattt atcaccccaa
    49021 gcgtactaca acgcctttgg tgcgccgtta tttgcttgaa ggcaaaccaa aacctgcagg
    49081 ccgtgaacag gtgtttgccg tcagcacaga tggcaagccc atccctgatt cgaagatctc
    49141 caatgattgg gattgggtcg aaacagattg ggatactgcg ctgaatttcg tggccgataa
    49201 ccttgtccgc atttaccagc gtgatggttc ggatgcgatg gcggtttatt gctgtgcaaa
    49261 agccaccaac gaagataatt atcttttaca gaaaatgtac cgcgccctat tccgcaccaa
    49321 caatgtggat cactgcacac gcctgtgcca tgcagggtct gtggtggcgc ttcagcaggc
    49381 agttggttcc tctgcgatga gcaacaccgc ttcacaggtc atcatgaacg atgtgttcat
    49441 tgtgacaggt tcgaacactt cggagaacca tcccattatt gcattacaga tgaaggaagc
    49501 cgtgcaaaaa cacggagcga agatgatcgt gatcgatccg cgccgcattg atttagtgga
    49561 catggccgag atgtggctcc cgctcaagcc tggcacaaat gtgcctgttt tctctgccat
    49621 ggcgcatgtg atcgtgaaag agaacctggt caatcacgag tttattgcca atcgtacgga
    49681 aggctatcag gattttgtgg aatcgcttga aaaattcacc cctgaatatg ccgaggagat
    49741 ttcaggtgtg cctgccgaag aaattcgcaa ggcggcgcgc atgtatgcag gtgcgaagaa
    49801 tggcgccatt tattggggca tgggtatttc ccagctttcc cacggtacag cctctgcaat
    49861 ggccttgatc cacctggcat ttctcacggg gcatatcggg cgtgacggta cggggctcaa
    49921 tcccttgcgc ggacaaaata atgtacaagg cgccagcgat atgggatcca tgcctttcca
    49981 ttaccctggt tacatgcgtg tggataaccc cgagaatcgt gcaaaatggg aaaaagcctg
    50041 gaacattgaa gaaggtggtt tgagcctgaa acttggcctg accacaactg aaattctcag
    50101 ccatgctcac gaaggcgggg ttcgggcttt gtacatcatg ggtgaaaatc cgatgatgtc
    50161 ggagccgaat ctcaacgata cacgcaggca tatgatggaa ttggaattcc tcgttgcgca
    50221 ggatatcttt atcaacgaga gtggttcctt tgcagatgtc ttcctgcctg caactccttt
    50281 tgctgaaaaa gatgggactt tctcgaacac tgaccgtcgt gtacagcgtg ttcgcgcggc
    50341 tcaggcccca cgtggaatgg cccgccccga ctggcagatc atctgcgatg tggccttacg
    50401 catcgaatct cgccttggca ggcccactgc gaaatgggat tacaccaacc cggaacagat
    50461 tctgcgcgaa atgggcgatg tcaattccga ttatgcaggc atcacctacg accgcattga
    50521 taagatcggt ttgatctacc ctgtgccttc gatggatcac ccaggcacac cgacactctt
    50581 caaagaatcc ttcccgcgtg ggcgtggaaa attccatcag ttggattatg tccctgtgcg
    50641 tgaagatgtg gatgatgaat atccgtttat cctcaccacg gggcgtttgc ttgaacattg
    50701 gcacggtgga accatgacgc gaaattcatc gctgaatgaa atttaccctg aagcccgtgt
    50761 ggaaattcat cctgccgatg cggagatcca tggcattcga aacgacgacc ctgtcaaggt
    50821 ccagagtaga cgcggcgagg ttgttgtccg cgcgacagtg accgagaaaa ccaccgtcgg
    50881 tgttgtcttc cttccctggc atttccacga agcggcagca aacctattga cgaacgatgc
    50941 gcttgatccg caggcaaaaa ttcccgagtt caaggcttgc gcggtgcaag tctttccagc
    51001 acgggagagt gacctgccga atcctgagat tgttttgaat agaggcagat attagaaaaa
    51061 gcagccctga gaaaatctca gggctgcttt ttgatttgga tgcttgagcg taaaaaggtt
    51121 tgtttattcg cactcaggta atgcaagggt ttgcgtgatg cacccattgc tggcggaagt
    51181 gacaggggca cagcaacctt cggccgcatt caagccgaac cctgcctgac attgatagga
    51241 tgtttccagt gaattggtgc aggctgagtt gcagaaagag acgaggaaat ttgtatctgc
    51301 agggcctgtg caggaaattc gcggctcacc atcgctgttc atatcttcgt tgatgcagtt
    51361 gaacccgggt gtaagcactt caaaggtggt gccaggtgac aaggtgtaca ggccatagga
    51421 tccgcggtca atgcagaaca gatcggtgat ttcgtaggtg ggttcgctgc aacttggggt
    51481 ttggctgtct gtgcaggccg ccagaaactc aggggtatca ccaccgcctg ttgagggaat
    51541 cagggttgtt tcttcaggga tggtttggaa tggctcgcag gttgagctgt aatcttcggt
    51601 tggaaaatac tgcttccatt gaatatcggt caggtcgccg ccagtgatgg tgcaggcttt
    51661 ttgcacccat gatgctggag tgatatccca aaaaatgatg ttgtcgttgt ttcccaaaga
    51721 tgccaggaaa acacctgacg gatcaaaatc aatcgtgccg atgttgccgc catgcccgac
    51781 cattttgctg atcaaaacgg atacttcagg gtttgagata ttccaaaggt ttacatactt
    51841 gtcgtaactg ccagaggcca attgggttcc atcggggctg aatgccacag atgagatcca
    51901 gtcagagtga ttggccagag tatccatttt ttgcgggttg atgggatcat ccacattcca
    51961 caagataatg gttttatcct tgccagcagt gacgagcagg ctgtcattaa ttgggctaaa
    52021 tgcggctgcg agaacctgat tggagtggcc ttccaacact cccatgcgtt tggtgttggt
    52081 gacgtcagaa atatcccaaa gaatgacatt gtttgctgct atgcctgcca gaaggttgcc
    52141 atctgcattg atggtcatgt cactgatctc attggattcc tgtgaaagtg tggagatctt
    52201 aacaggcttt ttggggtcgc ttacatccca aaatgaggct ttttcagtgt tgatcgcgag
    52261 agttttcccg tcgggagcga attcgatcgc gttgatcgtt tctccgctca atttgatcgt
    52321 gttcagtttg gaaaagtctt tcatatccca aagggttaca gtttcatcga aattcatgga
    52381 ggccatcaaa ctgccgtctt tgttaaaaag tatgatgctc gcagggccat cctgagtttt
    52441 gatttttacc gggcttgaaa gtttggaaat atcccagctt gtaagtttgt ggtcgctgtc
    52501 aaccgaaaca aggatgttgc tgttgggttg aatggccatg tcgatcattg tgccgtcaat
    52561 ggcgccgatt tccatttcgg tttttggttg agccgaatcc cagaccatga tggagccatc
    52621 gcggctcccc gagaagagca gattgttgtt tgcgttaaac agtacggtgt tgacgcccag
    52681 tgagtgcccg ttgatggtgc ggattttgta gggtacatac ctgtctgtaa catcccacag
    52741 cacgatctgg gagttggttt cgttgctgct ggaggccagt gtgtttccat ctgcggtgaa
    52801 ggccagactg ttgattgaaa gagagtgtcc gctccaattg ctgtagatcg tgggggttgc
    52861 agggtcggtg acatcccaca atacaatcag ggtgtttatg tcgcccaatg ccagtgtgtt
    52921 ttcatcgggg ctcaaagcga tggcggtgac ctttgatcca aacccatctg ttgtagagag
    52981 gactgtgggg gcgttggggt cctgcagatc ccaggcgacg atgcttccgt cactgcgtgt
    53041 gaagaacagg atgttcttgt tgcggttgaa aatggcactg ttgatatcca ggctttcgtc
    53101 cccgctttcc agttgtatca gctcaacagg caatttcgga ttggacacat caaataaggc
    53161 aatcgtgttg ttgacatcag cgtgaatgtt taatgatgta aggatttttc cgtccacagt
    53221 gaatccgagg ttggtgaaat atccgtcggt tttgctgagc agtgtcgggg aggatggatc
    53281 ggagatgtcc cacaggatca attcgtagtc ggaggaagtc gcaaggattt ttccatcagg
    53341 agtgaaggtt acattttcca cggggccagt gaattctttg agcgtggaca gtttcacagg
    53401 aaaatccgga tttgacatat tccagatgtt gacggtgtaa tcccagctgc ccgaaacaag
    53461 gatggtttgg tcggggttga gcaccatggt gttgacccag tcttcatggc cgagcaaaat
    53521 ttgatgcaaa ccagggtggc tttgcaagat cttggtgatg gcactttggg tgttcacgtc
    53581 attgatttta ttgtccttgg cgtgaaggta ggcttccaca ctcaggagca gggcctcatt
    53641 gtagctttga tcgatcttgg acatggccag cgctgacaat tggttgatct ttgcttccgt
    53701 ttccttgagc agtgcaaggt tttttgccgt ggcttcccgc tgttcacttt catttgccct
    53761 ggctgcttca ttgacggcca atcttgcaga gttcaggcca tacactgcca gtgcaaacat
    53821 gatcaccgct gcaatggcaa tgaagacggt tgtccacctt ctttgctggt cggtggcttg
    53881 gcggcttttt aagatgtatt cacggtgcag gtcggttgga taggggtctt ttgaagaatt
    53941 gagcgccaac tggctttctg cctcttgcag atcctgtccg cgcaggagaa agctcttgtc
    54001 tttgttttta ttcttccatt ccaacgcgcg gataagcagg cgtttatggg cctcaaccca
    54061 ttcaaaatct gtgttgatgc cttgcagtag ttttttgaag gatgtatcga agtcatcctg
    54121 ctcgcggaag aatatccaat tgaggtgcga gaacgctgcg gggacttcat cctctttaac
    54181 gtctcggatc atcaacggaa tcaggcgttt gttgttcttg accgcatgag caatttcatc
    54241 tccgcagacc tttgatttgg ccgaatcagg actgagaagg aatagaaacg catctgattc
    54301 ctcaattcca ttttcgatct tcttcatgaa gtctactgtt ggggggatgc cttcccaatc
    54361 cacccagaac tcgatctcac ttttttggag ttcctgggtc aggtgttttg taaattctgt
    54421 gtctttgcgg gaatatgaaa caaatacttt ggccatgttc tgctccattc gggccgttaa
    54481 ttgacaattc ctataataac ggcttgattt tacattgatt tatgtaaaca cgggcagacc
    54541 ttgcattttc agttcattgc gatgtgaccc ggggcttttt tcgagatgcc ttgacgttgt
    54601 acagcgccgt gtctgcaaac tggatgactt gttcaaccgt atcgccattt ctgggatagg
    54661 tggccgatcc agcggagaag ctgatcggca tctcttcacc tacactggtt attctggctt
    54721 cttgaatgcg cttcgtcaat tcttcgatac ggttttgggc ggtcatttca tcggcattat
    54781 gcagaatgac cagaaactcg tcgccgccat atcggcaggc agtgtcttcg atccgagtca
    54841 acctcttgat ctgtcttgcc agaagcttta gggcttcatc tccaacaaca tgaccatggg
    54901 tatcgttgag ggttttcaag ttgtccagat cgagcatcac aatgctgaat ggcttaccct
    54961 cgcggctggc tcgggcaaat tcctgtttga gcacttcttc catgtagcgg cgattgtaca
    55021 gccccgtaag cggatcttgg atcgcctgct cgcgcagaat ggattgcagt ttttcgatct
    55081 ccccaagctg cttgcgcagc tgttcgttca catcgcggag ttgatctgta atgcgtttac
    55141 gttcagtaat gtcgcgggtc actgcaataa atgccacagg caggccgagt tcgtttttta
    55201 ccagtgctgc gctcaattcg gcaggaaaag tcgacccgtc ttttcgcagg aggttgaatt
    55261 cgatgttcct gatcactcca tccgcgcggg tcatttccat gtacttgatt gctcttgcaa
    55321 cttcatccaa tgaaaaaaat ttaaaagcac tgaccccgat aaattcttca gaagaagaat
    55381 acccatgaag taccgcagtt tgcttgtttg cgaatatgta gtttccaaac gggtctacaa
    55441 cagcaatgga atctgttgag gtattcatga ccgcatggta aatcaaattg ctgatctttc
    55501 tggcttcgaa atccttttct tcatcgttta ggttattcca ccgccagcgt atggaggtct
    55561 gtaagccgag aattcccacg gcccacagca gggcaaaggc gggcaggttg ttctgaagcc
    55621 agttttcgtg aataaaataa acggcaagaa ccagaacagt ccacgcagtt ataatttgaa
    55681 gcaggaatga tttttggaaa tattggttga atctggcatc tttcatcttg ccctcgcctt
    55741 taagttttgt gaattatacg ccttttggaa acgcccatga ctagaaatta tctcgtccct
    55801 ctcaccgaaa ttcgccgtga gcaggaagtg ctgaaatcgc gcttcatcgc cacgcttgcg
    55861 cctgcttttt ccattgacga agcacgggct ttcatggcgc gtatgaaaaa agaattttcc
    55921 gatgccagcc ataatgtgcc tgtgtacgtc attggcggag ggaatacggt gacggaatat
    55981 ttttcggatg atggggaacc gagcgggact tcggggaagc ctgctctggc ggtcctgcgg
    56041 ggcagcggac ttggcgatgt ggttttggtc atcacccgtt attttggcgg caccctgctc
    56101 ggtacgggcg gactggtcaa agcatacaca gaggctgcgc aatctgtcgt caacgccgtg
    56161 gagcgcggac gtagagtccc tgtccatgtg gtgatgatgg cgattcccta taatctgctt
    56221 gagcgggtgc gtctgctggt ctcgaaagac cgcggaaaga ttctgggtga agattatgcg
    56281 ggggatatca cacttaccat gcaattccca gttgagaatt tcgacacctt tcaagcggac
    56341 atgcgcgaac tctcagcagg gaagattcaa gcggaagtga tcgaatcaaa ggaagagatc
    56401 gtttcagcgt cagcttgatg atcgactgcc tgcaaaatat tccacgtaaa agtgaacgtg
    56461 tcttgcagtg actgtctgtc aatgaactcg atgcggtcgg acatttggtg ccagtgagaa
    56521 actgcctgtt cagggttggg gaaggctgaa gagatgggca gggcgataag tccctgtttg
    56581 gtggcgggca gggcatcggt gtaggcttca cctttggctt cgatcaggct ttgtgtcaac
    56641 ccgaaatttg catttcgtgc caattcgagc gcccgtggat gcgttttgga ttttcggatc
    56701 aacccatctt ctgtaaccgt ttttagctgc cccgcgccga cttcgtcgag aataatataa
    56761 acggcatctt ttccaaatgc ttctgcatgt ttcttcaagt aggcgaggat gccatgtgcc
    56821 cctgtttctt cgcagtcggt aaaaatgaag ttgacctttg tgttcgtcag cggttctgca
    56881 cgaaggcgtt ctgcaagggc gaggatgatg ccagtgcctg aggcgttgtc gttggctcct
    56941 ggcgagaagg gggtgaattc agccgagatg accaggatca aggcatacat ttgcaccaac
    57001 ccaaaaagca ggccaaccca tcgaaattca ttgagatcca gcagggttcc caatccaaat
    57061 gtcagcgcgc ttaataccat gctcagaaac gctccagtga cggaaacccc aaaggctttt
    57121 tgccaggctg tggatgaata gaatatcggg gtgcggtgcg tgtcgagatg ggagcacagg
    57181 ataatttgtc gtttgggttt ggtttgcgga gaaactgccc cagtgacgtt aattgtttcg
    57241 gcggtcggaa tcagccagcg ggtccagttg gaggcgaatt cactttctgc gaacatggcc
    57301 catgcgccga gaaaattcat cagggcggcg attccgaatg tgaggcgatt tccaaaaaat
    57361 aaagcgaggc aggttccagt gagtgcggag gcaaaggcca gcgcaaaagg caggtaggtg
    57421 gatgccatgc cgcggaacgg ctcaaactca atttggctta cctctgctga tctcaaggct
    57481 tcggcagagt attctgccgc ctgccgatgg tttgggctgc aactgccgcg cccgccgatg
    57541 gtttctgaaa gatgacgaat gtgctcgaac gcgcgctgga cgtaatccat tttgaatgcc
    57601 tttgtattag atcttcctgc ttgctttttc caccagatat acgatggaaa cataatcttt
    57661 gcctgtggct tctttcatgc cgatctcaca agtgatgttg gaggagtagt ggccgccata
    57721 ttcttttgca aggacttcgg cgctctcttt ttcggtggca gatgcggtga gttcggggtg
    57781 ggtcagtccg cggtcacctg caaaggcaca gcagccaagc gcaaatggga ccactgccga
    57841 tctggcacat tgcctggcaa tggcgtgcat ttttacatcg aggttgagtt tgcgggcgga
    57901 gcaattggga tgcaggatca cttcttcgtt cacagggtgg atttcaagct tgggcaggac
    57961 atggtcgtgt aaaaattcca cgccgtcgag gagggtgagt tgacgccaga tctccctgtc
    58021 ttcgggggtg aggtcttcgc cgcaggtttt gagcgtgtgt gtgcaggagg tggtgtctat
    58081 gacgatggga tattttccat gctcagacca ttcccacatt tgcatgaggg ttttgtgaag
    58141 agctgcctga tatgcaggtt tgtatccctt ggaagagaag ggcatgccgc agcagtgacc
    58201 ctctgtttga tcggggattt gcagattgat gttggctcgc ttggcgatgg tgaggaaaac
    58261 tgtggaaagt ggtgagtggt gggtaggttt gccgagttgg cgcgaaatac aggaggggaa
    58321 gtagatgaat tctttttcac cacggagagc actgagtttt tttggagagt gcgggatgga
    58381 gtgattccac ttggggagtc ttgtgccagt tactttctcc gcggccgctg aaatagattt
    58441 gactccaatt gcgccaatta ctttctctgc ggcttgtccg agtgaaacgc caattccaac
    58501 cgctttttca accgttgcga aattttctac aatccactct gcggacttgt tacttgttac
    58561 tcgtttctca cgcagcttct ttataaactt tcccgtgtca attcccactg ggcaggcagt
    58621 tccacagaga ccgtcgaggg cgcaggtgtc gattccagcg taggtgtaat ctgttgaaac
    58681 agaatcaaat tcgggtgagg atgaatcggc gaggcgcatt cgggcaagtt ctctttggac
    58741 gacgatcctt tgtctgggag tcagggtcaa cctgcggctg ggacatttcg actcgcagaa
    58801 tccacattcg atgcatttgt caatttcagg cgcaacgagt gagagcgact tcaagtgggt
    58861 gacgtgtgct ttcgggtcgt cgttgatgag cacgtttggg ttcaacattc catcagggtc
    58921 gagcaatgat ttgaggtcac gcatgatggc gtaggcttca ctgccccatt cggtttcgac
    58981 gaaaggcgcc atgttgcgcc ctgtgccgtg ttcggctttg agagagccat cgtatttgcc
    59041 gctgacgatc ttcaccatcg cttgcatgaa attgtcgaag tggttgatct gttcaggggt
    59101 atcgaaagcc tggttgacga ggaaatgcaa gttgccatct ttcgcgtggc cgaaaatgac
    59161 accctcggta tatccgaaat catcgaagag atgacgcagg tcggtaacgg cgtcggcgag
    59221 atgttcaacg ggaaaagcga catcttcgtt gaccgaggtt gtgccgcgcg gacgcatcgc
    59281 gccgaccgat gcaatgatgc ctttgcgcgg tttccacaat gcggcctgct ggatgggatc
    59341 gtcggtgaat tcgacatcat gaatcagttt aagcttgccg atgacgcgct gcgcttcttt
    59401 cttaaactga agcatggatt ctgaatctgc ggcttgatac tccaccagaa tcgccgccgc
    59461 gccattaggc agttgcttga gaatggcagg cacgcccgcg ttgttttcca ccgcccgcag
    59521 ggactcccta tccatgatct ctgccgcgcg cgcacccgac tgtttgatgg ggtaaattgc
    59581 cgccgccgcg tcacggatgt ttttgaaata catctgtcct gtgtatttgt gggtgaaatc
    59641 gggcaaggtg tggagcacgc cttcggcgat gaatccaagc gtgccttcag agccgatcaa
    59701 caaatggacg agaatatcga gcggagaatt gaaatcgaga aaggcattga gcaggtagcc
    59761 gttgttgttc ttgatttgat agcgtttggc gatgcgggtt gaaagtttca ggttggaggt
    59821 tacacgtttt ttgagttcga gcagaccttt ggcgatctgc ggttgttcgt tctcgaaaag
    59881 ctgatttgca ttcgggtcgg ttgtatcgag cacaaatcca tttggcagaa caaaggtcat
    59941 cgagtggatg gttttgtacg agttctcggt ggtgccacag cacatgccgc tggagttgtt
    60001 cgcgaggatc ccgcccatca tgcacgcatc aatggaagca gggtcggggc cgatgcgccg
    60061 tctgtgcggt ttgagcacgt tgttgagaaa tccgcccacg atcccaggct gaaagcggat
    60121 tcggtttaca tcggcttcca cgctgaattc cccccaatgt ttggaaatat ccaccagaat
    60181 cccatctgtc actgcctgcc ctgaaagact cgtccctgcc gcgcggaagg tcatcgggat
    60241 tttattctgc tggctgaatt tgaaaagcga ttgaatttcc ccgatcgaat tcggctgcac
    60301 caccgcctgc ggcaccagcc gaaagtacga agcgtcattg gcataagcga tgcggtcgat
    60361 caaacggtca cggatgcgcg aggcggggag gatggtggtg aggttcatgg gcatattata
    60421 agac
//
LOCUS       DAOQJC010000006        49776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_283_length_49776_cov_8.752056, whole genome shotgun sequence.
ACCESSION   DAOQJC010000006 DAOQJC010000000
VERSION     DAOQJC010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 49776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49776
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_283_length_49776_cov_8.752056"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLE39_01660"
     CDS             <1..391
                     /locus_tag="PLE39_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67057.1"
                     /translation="GSIELEKKQGSVPGFGDGGMGGANDEKDLLSHIITTLNETYGLN
                     LSEDDKVDMQTIRTRLEADEALQAVLSAQNTRADKVYKFNEVLDRLILEFVHGKLDLY
                     KKLTEPKVNDMLKRQWFEKFVQEFGKA"
     gene            613..684
                     /locus_tag="PLE39_01665"
     tRNA            613..684
                     /locus_tag="PLE39_01665"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:645..647,aa:Gly,seq:ccc)
     gene            755..1186
                     /locus_tag="PLE39_01670"
     CDS             755..1186
                     /locus_tag="PLE39_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE67058.1"
                     /translation="MTYSKIQVGGSRMIPETKKGTILYVEDNPDNRSLIRRVLESEDY
                     SVIEAVNASQALENLEKGNIDLVLMDINMPDMDGYTLTAKIKSVQKFTKIPIVAVTAN
                     VMRGDREKSLEAGCDGYIQKPIDIDTLTQQIERYITRSSNV"
     gene            1179..1631
                     /locus_tag="PLE39_01675"
     CDS             1179..1631
                     /locus_tag="PLE39_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE67059.1"
                     /translation="MSDQIPAISDTQPIRKPNIPKDAAVLVVEDNVSNFVLIARMLGY
                     LGIHCEWKTSGYEVVEYADTLPRLDLILMDIRLPYEDGYGALKKIRASERFKAVPIIA
                     VTAEASMEQMAKAKDSGFDGFLGKPLDPDRFPDQIRRILSGEPVWEYS"
     gene            1756..5517
                     /locus_tag="PLE39_01680"
     CDS             1756..5517
                     /locus_tag="PLE39_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67060.1"
                     /translation="MAGTAYFRTRTLLREQANNQAQNLFVSQLNIINREVGDKEVHFQ
                     DLLASSDFQIMLELALHSDPKTDEFQVLRDGILQEFSAMNRHENMPAFDQFLLLDTNG
                     NIKIATEEKWQGETLDPAIIGQDFDTQHTLALYGLSPLYEEELLLVTTLRYETKQGAV
                     LGFVVGITEKKSIQNSMQSLSGLPMSSAYLVLSNQQFFQSQPEANRFTLLTSPLEAQS
                     ELETALTESRLDGGVSPRSIETTTAENKIKVFQVQWVPKLQVWMILETDTTEAYKSLT
                     SLAPFTAILTTITLLAIGLALAITINRVIKPLQSLSQIAHSFADGDLSKRAEIKSDDE
                     VGLLASSFNHMAEELGSLYRSLEQTVDERSRQIRTAAEVAQSVTKLSNLDEMLDKTVE
                     LLVREFGFYQASVFLMDPGGKALEFKTGFGSATSALIKRKYRLEVNSESIVGWVSTNN
                     QPRIASDVSEDPIHLKNELLPETRSEACLPISIGSLVLGVLDVQSIQVGVFDTETVAM
                     LQTLASQIATAIQTTGLTETSQINFEELAQLYRSSRLIAEANTENEILEIGRDAFADA
                     PYPVIILRVVNNKLEMFSQTDGKSGNSVKKISLPSASEASVVDIHNYLHNGQVIITSE
                     DKNIPAVLDSIMQTLRFSSAAVFPLRRGGLLHTVIIMGGQQLFSNAIVQPYANLADLM
                     SVTMEKLDAIQQTEKHLREVEALASISEVVATSSDMQSFFMNLHAKIRQVIGDYAFIV
                     ALYNERDNTVSIPFAYEDDKITSIESFPLGEGLTSILIRTRQPLMIVENAERRMIELG
                     AKFSGKPGRSWLGAPMLVQNQPVGALIIQDTEKEHAFDEEDLKFFNTIAGQVAGVIHN
                     AHLLDESQNKALQLETAAEIARDISGSLNLDELLIKAVNFIRERFDFYHASVFLHDLP
                     GEFAVIREATGEAGAQMKRSGYKIGIGSKSIVGYVSSRGERLVVGDTTKDATYYANPL
                     FPDTRSEAAVPLKVGERILGVVDVQSTRPYAFTEDKLRSLQILADQIAIAVVNTELFA
                     ETQEHLSQHRLLHHITTTAASGTTLEEALENAVNGLQVTLGGDRVTIMLANREKNLLE
                     VKASMGYAEDIAKVQVPIGSGITGWAAAHKRPLRIKDVSDDPRYIRISSNTRSELAIP
                     LVYRNELLGVLNVESEQTDAYTENDEEMLGTLGGSLAAIIANARLLEQIRIQAERERL
                     IYEVTSKIRRSTDIQSILNTTASELARLTGAQYTKIRIQPGNDPTQEGN"
     gene            5517..7112
                     /locus_tag="PLE39_01685"
     CDS             5517..7112
                     /locus_tag="PLE39_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67061.1"
                     /translation="MMESYPSTTNASNSPEEATRLLYKSWREQFTMPLLIGSLVLGIL
                     ALIPALSGAGNTFIRVLFISVYVLTGIVTVIRFSYSIRMSVFLTSIFALALAELFTHG
                     ILGDSLFFFLGLIVFATLMVSPKTGIAAIIVNILTFVAVGISMQSGRLVPINPNAPPS
                     NISDWFSAGAAIILFGVVIVLGFQRLEVAIFETQKQIQSTLSALKHERDNLETSVSDR
                     TQLLQKVNRVGRAITAILDPNELLSHAVYLISDELECYYTAIFIVDATNQWAELRAAT
                     GDAGRVLIENKHHLNVNGKSIVSTVLRTKQARVALDTGADPVRSDNPLLPYTRSQIAL
                     PLTIGDRVIGALELHSTKENAFMQQDVDAYQNMANQITIAIENSRLFTEAQQSLAEMS
                     ATQQQYLKGAWLSLSSEQKLEYAVGDSESSDKQEIEVPLTLRDQVIGQISLSSASDWT
                     SEQKNLIESVAVQAALALENARLVEESQSVATRERLINEITSKVWASTTIDSILQTTV
                     RELGRALEAAEVDIEVTMDDDNE"
     gene            7105..8766
                     /locus_tag="PLE39_01690"
     CDS             7105..8766
                     /locus_tag="PLE39_01690"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67062.1"
                     /translation="MNSKTPITTSSAFDYIGWREQFIITVLRIACVLGAALIATSFPT
                     ASATDRVLFPSLYLILLAITVLKTPYSVRAASLLFMVYIIGTNSLLSWGPWLDGSIFF
                     IAFTILAALLFDNRIDLIALILSALTFVTLGVLQQLGIYEPSAKGVPPTIATDWAAYT
                     VDFSIVSAIILVATNQFKKALTQVIFEMQSAFNSLTTEREKLEDRVRERTEELEARAI
                     QLRAATSVTRTIAGIQDISELLSETTRLITDEFGYYHSGLYLLDERKRTTFLQASSSA
                     TGKSLIGQGFRIEPNKLNMTNLVIEQRRPQIAMDVGDANFIRDPNFPLTRSRIMLPLT
                     VRGQVIGILDIHSDQPRTFDGQDAEVLQTLADLVAISIDNVRLIDETKSLVYELESNT
                     TLQTRETWSKFTSRHKPAYQYTPAGVRPLFSKIKTEKADGLHVPMILHGQNIGNIVLK
                     RKGISSTWSERERILVEKVAAQVALALENSRLVDETQKNAMRNQVIANISSRVRETLD
                     VESVVRTATTELRRVFDLKEAEISIGAPQPEPTQLRKNTSSLRLK"
     gene            8795..11158
                     /locus_tag="PLE39_01695"
     CDS             8795..11158
                     /locus_tag="PLE39_01695"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67063.1"
                     /translation="MRTRSLPTSTKSLNKPPRRGLFGRYVSLQVKLPVVVITLLLLAL
                     FASAYLSIQAAKTALIDTLKNELILQADSKAELIRSNLIWTRAAAIDLAASAESVNYD
                     EETIQKAIRNTLLRNEQVFGSTIAYEPFQFHSNSYYWAPYYSRAANGELQFTQLGNPE
                     YNYFGWEWYTLPKEKNTPVLSPPYFDAGGGEIWMVTWSAPFYNEAGEFKGVATADIAF
                     SQTQDIVSKIEVGKNGYAFLLSPEGVILGIGSNGGQYQTMVDSMIAAAQSSSQASGWA
                     SLVTSMIQGSTGFSEAVDPQGRSMFIAYSPIGLDTGWSLGLAFPQAELFQRAVQLQNT
                     LGVYTLIIAVIFGVFLFVYTLSITNPLRRLAWYVGHFSPEKINQNLEPVQIYTRDELE
                     DLSTTFQQITTNLFNTFTNFEERIAERTRETERRSILLKAVADVGKAITSFRDLSELL
                     QQTTYLINENFGYYHIGIFLLDEHKEYAVLSASNSEGGRRMLEKGHQLKVGETGIVGY
                     VTQNAKARIALDVGKDAVYFDNPDLPDTRSEMALPLIIGGQILGALDVQSTEAQAFSD
                     EDSATLQILAEQLAVAIQNANLFNETQKALEASRAAYSELSREAWTKILRNQPRIGFI
                     ATPPTTTRTYSDTVEPSIAKAFDTGDIIIGSDNLTISIPVKVRGQTIGAMRLKKAEIA
                     EAWTQEEVNLAISLSDQVSGALESARLYRDSQQTGARESLVSDISSRISAVSSTDAIL
                     RETVQELGQVLGDASITFQLLNQPDGQKQTEVELRSGLSGQAGKAKE"
     gene            11163..12917
                     /locus_tag="PLE39_01700"
     CDS             11163..12917
                     /locus_tag="PLE39_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67064.1"
                     /translation="MSISKPPLNTQSASRFERIQQIGQSLIAPAPSIKDIGQFRRAQL
                     LAILTLITSVLFFAAIFSGPRSYGVFIALTLISLASYALSRTTSFRTGTYLFTYAFTA
                     IGFIRIYQGEATSIEASIVSTVHVALVVSSVLLSQRGFLGLVALSTIATFTAPFYADA
                     TIAANSAQAFTSDISRTGGIVFTLGILLYGIQVFRANLDKEKLKELLDTNQELEEVKK
                     NLEQRISSRTLELEKANQQVQERAARFQIVSEISQEISANVEQKPKDLLQSIARSISE
                     KLGFYHVGIFLLDKNREFAVLRASNSQGGLRMMDRHHQLKVGGAGIVGYVAQAARPRI
                     ALDTGTDAVFFNNPDLPKTRSEIALPLKYGSRLIGVLDVQSTQQFAFNDEDANLLSTL
                     ANQISIVINSIIAAENGELGYSQGYFQAEKLARKKDTQAGFSYLPDGSIVTARPLNDL
                     ATEQALASGETVVLSQSSRKVPPTLAIPVKFRDQVIGVIHIQATEKNRKWTEDEVALV
                     QSISDRAALALENARLFEETVRRADQEETIAKVTTQIGASTDFNRILQTTIQELGQVL
                     GVSRSFIQMGPLKNEEEQ"
     gene            12943..15288
                     /locus_tag="PLE39_01705"
     CDS             12943..15288
                     /locus_tag="PLE39_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67065.1"
                     /translation="MSQNTPAQTTKTFTGRENNLSLQTRLVLFVLFIALVPLTIIAVR
                     DNLQTQQALTNSAEISLQSSAEQTANSLDTFIQTTLNSIVIESQFSVFTEYLSLPNSQ
                     RITGRPEQFRAQGLLNKLSSKDSQNIISYAILDVDGNILLDSAPQRAKKNESDEAYFP
                     QVRFSTQPVITEVAYDENGNKTITFANRVLSSNGTYLGILRVKYSSNVLQNIIVDSSQ
                     TSANALVLLLDEVYIRLADSRNSGLIQKSIAPLSPVDYLQAVESKRFLNTSPEEQSTN
                     FVEFENALDNASVTPFFRVDITPNIPGDDTVAVAPMKTRPWVVTYSRPTSLFLADVQT
                     QTRANTALVIGALILISILTTVVARNLTAPIVALTKTANLISQGDLSARAEINTTDEI
                     GQLASAFNSMTNQLSNSLSGLEERVFERTADIQRRNLELETISDVAREISTIRDLDTL
                     LNVSTSLIREKFNFYHVGIFLVDERGEYAILRAASSVAAEELLARKYKLRVGQTGLVG
                     NVTSTGQAYIALDVGRDAVHFENPFLPNTRSEIVLPLRNYNVTIGALDIQADVPTAFD
                     ERDMQTFQILADQLAAAIENAQLAHQVAENLAALTNANRFQTQQTWRTVIEQQRHSAY
                     EYDGLQIKAVPQDLPADIMKQLENGNPIVYQQPTQGRDVKHILMVPLIVFNQVIGVIG
                     LEQEKADHNWTDEDVTIAQAAANRAALTLENARLLEESQRRAIKERAIFEATSRIGSA
                     LSVENILQATAEELERVISGSEIILQFNTEGSSGINDKDEL"
     gene            15325..17025
                     /locus_tag="PLE39_01710"
     CDS             15325..17025
                     /locus_tag="PLE39_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67066.1"
                     /translation="MNQPSENLDFSYTRWQASFLQATLIGACVFGLLAIIPAVLGTAD
                     PILIGIYIGAYIALLLITILPAPYTIKAVTLVGLLFLLGISGLLETGIRGDARLFMLA
                     AVTMATLLLSWRAGWIMTGLSMASFIIAGWLIVTGAITISSKNVTPGNFDTWISAITA
                     ALLLEVVIVNGIRLIQVEFESSRNRVRESFQKIEEERNLLEDRVKQRTQDLGRINRIS
                     EHRAQMFQTIAQVTRAIISTQNLQDLLPQIAQVISQQFGFYHVGIFLLDSNKEYAVLS
                     ATNSEGGQRMINRDHKLRIGQTGIVGNVAGTGKPRIALDTGLDATFFNNPDLPETRSE
                     IALPLFRSGQELIGVLDVQSTESNAFTQDDIQILTTLADQVSIAIANARSYEETQTVL
                     LESQLLYRRNIQAGWSKFVRSQKLSGIRKQGLKSSLLFDPMEVPGAKEVVRSGTIYKI
                     SEDDQNTSLTIPMKLRGEVVGLLNIKTEDENAIGTDELDIINAIVERAALSIDNARLL
                     AESRKTAEKERVIGEISSKVSSFTNRDNILQAAVAEIGRALPGAEVVIQLQKKNDTDS
                     "
     gene            17044..18771
                     /locus_tag="PLE39_01715"
     CDS             17044..18771
                     /locus_tag="PLE39_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67067.1"
                     /translation="MSSNWFADLEDTNPSFLRMTRNILVFTIIANLAILLLVVGLFFR
                     ESQNAASSVALSVTLVLEIASLIFVLRGNPIPAKIVVPVSLFVAVTYTAINANGLHDL
                     SMLGFPTVIVVSALLLRKRALIISTPIAVLCVEIVAFADMTGITNSEMALKTDVSDAL
                     IAGILMIAISFLLQLLITRLNESAEEARNNAALQVEANKELLSLQANLENRIESRTRE
                     LNEVNAFNERRAANFEAAAQLARTVASIRDINKLLPAIAEVISARFDYYHVGIFLNDD
                     TQEYTSLVAANSEIGKQLMAGGLQYKIGQAGFVPSVAQTGYSRVSQDHNQIKSVSDSQ
                     EFQKTQSELVLPMKIENKVLGVLDMHSDKTYAFDEVAIEILSILADQTAIAIQNARAY
                     EETQAALAESQILYGTVIKQAWQTNVRVENKLGYRFSGTIPMLLEKPVDSQELQAAFE
                     SGKTVILASEDQNRADGSVLAVPLILRGETVGVIHVKTPADIDMGEDEADIVRATAER
                     VVLALENSTLLEESQRRALREQTIGQISSKISAGTEIEMILKTAVRELGSQISGAQIS
                     VEIGSDNDE"
     gene            18764..21721
                     /locus_tag="PLE39_01720"
     CDS             18764..21721
                     /locus_tag="PLE39_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67068.1"
                     /translation="MNRDTNMERASNSQQVDSVLVRNAYTISIMLLITAIPSVGLFVY
                     YALLKGSAQILTISIIMFIASFGAVAALFLIRRGRSNLAMMIIITSFTITFVAIPFFI
                     KGLGVILAISMFILAVSITGLAMPTRYAASGLIVGLSSGALILLLDYLFLGEARVRVP
                     ELERATVFIVVGFALFFGAFTVRHFNRFSLRIKITISILLTGGVIVVTLVWFAASRTG
                     NIINTLTTRYETASRENIQNQLLHTVEDRAAQTNGFFDKLQNDLGILAKYRASLEQQK
                     DIFIDSTYWDADNKVIQLTEGQYGNSPADVASIFIPSTVVVDQSLYNDLNTTAYLDFY
                     APNFLQTHSSVIALYFINTAGATTYYPNIRLAENVPADFDPTKEIFYTIASPENNPSR
                     EPRWTDTYEDPAGNGLITTLSIPVYQNDTFLGVIGLDVQLAQIERAIANVKVGDTGYA
                     FLVDKAGHILSMPPEGYLFYNISPEEVTTNSVHELSVLSKGPFDVQEATAQMINGDSE
                     IATIKNGDGNSFFIFSPVTVPNYRLGIVAPENEFTTEFVQSRSEIEDIVQSALQGVAI
                     ILIVLLVGAIIVSLWIGQLITRPLVRLTQTAEKISQGDFAARALVETGDETGVLAKAF
                     NSMAEILNSTLLGLEKSILERTRELEEANQSIIRRAGQFEAIARVARTISSTQTLNSL
                     LPQIVDTISQEFGFYHVGIFLVDAHREYAILAAANSEGGKRMLNRNHRLLVGGTGIVG
                     FVTNSGQPRVALDVGMDAAFFNNPDLPDTHSEIALPLLIGTQVIGALDVQSRETDAFS
                     QEDVSILSTLADQVSIAIQNARSYQQSRDALDQAEAASMQMSELQWGRFFAEQPSNGF
                     VFDGLDAKDIKQVKTKDENTYTIPLILRGTKIGTLKLNTDNPNRTWTDDEIAMAQATA
                     ERTAFAIENARLLQEAQKRAAKERAIGQITAKIGGLINIESILQTSIQELGNTIPNTD
                     IAIQISPEELEQK"
     gene            21740..23470
                     /locus_tag="PLE39_01725"
     CDS             21740..23470
                     /locus_tag="PLE39_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67069.1"
                     /translation="MLLKIINYFRDEEDSDPSFIRLVRNIVIFVLVVNIALLPLVTGI
                     VGEGSRNPRAFFTLLTTLILEIISLFLVTRGKLGMAKVVVPFALIAAVTIISLTTNGL
                     KNAAMVGMPIILVISAILLGRRSIYLTTPVAIIAVTIVAVANLQNQIPIIPAGVDDAI
                     ILPVLLLGTAAIVQLLITRLNDSIKRARESEQIQKTENAELNELRSMLEERVNQRTSE
                     LEFANRNNERRARQFEAISQVVRVVSSIQDLELLIPRIAQVISEQFGFYHTGIFLLDD
                     EREYAILRAANSEGGRKMLERGHKLQVGQTGIVGFVTATGQPRIALDVGSDAVFFDNP
                     DLPNTRSEIALPLRYAGQIIGALDVQSTEANAFGQNDVEALTTLADQISIAINNTQVL
                     EESRKAFAESQSRFGDAVLESWKIMQPESLGLGYQLVNSSIKPLDVPLNGDHIQEAIS
                     QEKPILDNPEGKVSQLAIPIRMRGKIIGIMNLSSKNDYVLTKDDADIAEAVSERLSLA
                     IETATLLRTTQHRADIERVTADISSKISSSTHFDAILQITAQELSKALGGSDVLVQIE
                     PVSMELGMSS"
     gene            23489..24457
                     /locus_tag="PLE39_01730"
     CDS             23489..24457
                     /locus_tag="PLE39_01730"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE67070.1"
                     /translation="MRTPISKFLPVLLLTVIFSACTAISQPVSAPLPPLRVEFTNWWG
                     DYTLLVAQEKGFFEKYGVQVEPVYYEFFSDTYPDLAAGQLDAGLIAVGDTINISRIAP
                     MKVVAVHDNGGDDAMVARPEILSIENLRGLTVGTQIGSQYELMITEMLRSANMDIADV
                     KVVSIYPEDGLAALENNQVQAVYIWEPYLSEALSKGYKVLYPKEQLHLFPDLIVFQKS
                     VTDQRPDDVRAFLKAWFEAVDYRLQNPEETRAIAAKYLGVSVEEVQPDDNLKILTLED
                     NKAIFRLESENSIYTVTKITSDFLIAIGTITQMPDPLELLDSRYLP"
     gene            24485..27304
                     /locus_tag="PLE39_01735"
     CDS             24485..27304
                     /locus_tag="PLE39_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67071.1"
                     /translation="MNVKNTSDQQEEARLKRNALGISLITGILFLVVSVFAVIVGYQE
                     NGMNGLWGGAITGFISLAAFFSSIQAYRGRTSIGIGFLIFAILLVSFAIPIVAQGHGL
                     ALSILVLVVVASISSTTLSAKWTTRSIIIAFIVAAAIILIDLYLPDLGLPGNPTYINI
                     VALVTSLAYIAFILRSFNTYSLRTKIITAFTLITITPLVTLGYFYSLSSTRILENQSK
                     LQLTNIAKIQAESVDNFITNQIDSVHADAKQLAFVEYLRLPAAARSGSVKELNARLAL
                     LALTRRDPVFIHSIAILDTDGTNLIDTFEDYKGQTENSLIYFVRPMQTGLPFASNILF
                     QNNRNHIYFSAPIKSQNGNTIGILRVEYHATIIQAIVRSVDPLNAGTTLSLVDTNTYL
                     RVAYTGDRDELFKTYKDFSAPDLAVLQSEGRLPSISIESLLTETDVAMVDGIDNLQQQ
                     SFFDATSKSLGSTTNTGVFLETQPWVAIVRQSKSNYLAPVEDQNRTTILISLIMVILS
                     IGAGFLASQVITSPLLTLAKTAEQIAVGNRSARAEAVTEDEIGLLAKSFNRMTDELNQ
                     AFKNLEIRVVERTTDLEIARRQSEKRASELQAIGEISKVITGEQALEKLLPLISRLVS
                     ERFGFYHTGIFLLNETNQFAVLQAASSEGGQKMLERGHKLEVGESGIVGYVAKFGMPR
                     ISLDVGHDAVFFNNPDLPNTRSEMALPLKVRNRIVGVLDVQSEKPGAFTEYDASTLSI
                     LADQIAIALENARLFTQNQQALAEAQAQYRQNLQSSWRVFSREEETIGYHQGLMGGKK
                     ISKPVETEEIREAINRGNVLVLNSETDKREATIVVPIKLRGQIIGTMNIKAPSANRTW
                     SRNEVNLAEAISERLSLALENARLIQESQRQVIKEQTISEVTGKIGASINLKSVLQTA
                     VEELGHAIPGSEVLIEFEQKNENME"
     gene            27312..>30090
                     /locus_tag="PLE39_01740"
     CDS             27312..>30090
                     /locus_tag="PLE39_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67072.1"
                     /translation="MTAAYSETPIFQNREQEQRERIALTVARAFLVTSTIISFSILYI
                     AISLNSWQLFTLYGGNILYILLTYAGLQQLQKKNTLFGIFLVTGSVHLTIILLPILFE
                     GIATLAGLFSTLAAALILSQTLETRVARGAIVVSNITGIGIILLGSIPFPVRIQIPSV
                     LVVTIAILAIIETAIFMVLIWRQFRTFNIQTKLTLVFITILVTSLLVTGGYNAVQSYR
                     TQRAQTANNLENELGVKRSVIIDFLEASRQDVVFLGEAEVVQSYVKTIDDIAAPNIVV
                     RARTIMEREFRRFAESRGIYQEIRFLNNDGNEVIRIDTALDGNSSIVPQAELRQKGEL
                     DPARELSYFAQTITLPAGRLFQSPINLRKLDGTIVTPHEPIIQFGVPLVINTQTKGVI
                     MANIYAEKFLGILSASGDDTFLVDTNGYYIAHPDESKRWGNDLNNNITIKNDFPDLVN
                     NLNNGNSGSMESNGYLVIYTPITIPGETEPRWYIGSFVSIDSITKPIFESTYNALSLL
                     LLTIIASIFIMSTLSNSITSPLGNLALAAQDVAAGKLSARVNLQTNDEIGALASIFNS
                     MTDQLRETVSSLEARVADRTAELAKEKQQSDTRAKQFEAITKVARAISAKSNLQDLLP
                     QISRVISEQFGFYHVGIFLNDPANHMAVLSAANSDGGKKMLQRSHQLRIGEQGIVGYV
                     AQTGNPRIALDVGEDANFFNNPDLPDTHSEMALPLKASNKIIGVLDIQSTMTAAFTDE
                     DFEALSALADQVSLAIENARLFDETEKMLAESRAIQRQNTLETWQRLPQEENLRGFRY
                     SIIGASPLKTSETNSTQVDTSEKRIIKVPINLRGERIGTLSVQVAKHEHVSSDQLDLI
                     KAVAERVALSAENARLFEETTRRAEQERIIAEITSRIGTSVRTENILRTTASELSQLL
                     EDADIYIELQSK"
     assembly_gap    30091..30190
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            30280..33102
                     /locus_tag="PLE39_01745"
     CDS             30280..33102
                     /locus_tag="PLE39_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67073.1"
                     /translation="MQEKSSYHQTSPNKQQLDRNAFNISVITALLLGLALGVAYLLSP
                     TSLDTISLVGLLAGIFSAGTSIWLSRRQKSEIGIGILITALIAISVSQVFVRKGQALA
                     TSITNIIIISGISFYTLRRKWAGWAIMASFLMASVTVVIDQFTTGVPESSRPEAATAI
                     AVGLSLIYLVIIALQFKNFPLRVKLIFGFLVLTTLPLLILGWQTNLATREILREEIKS
                     SILESTLSISDVFQEFVDSQHVAIGKQARTPELTDFLSLPAPERADSDLEAAALETLT
                     KFKDESASHIIAYSLVDLGGRNILSTDPSAIGVSYTDQAFIASAITDHKPHASGLYLT
                     HQPDVRIIYFATPIVSKTNDSLGVLLVTYNAKILQAVMDTILRGRPVINTDQYSFLID
                     KTGFFVLAHSSFLTLDFKTYLGENDPRLKVLQEQAGTDLDAIKAFNIPQPEIVEAIKS
                     APEGTSSFQIPSVENDGKLAESAANTISNSDWVLVTSQPVSTISKATSGQTRNTVLIS
                     IAIVFIAAVSAVAVSNVFTNPLQQLTSVAEKISAGDFSQQAEIKTHDEIGLLAGAINE
                     MASQIRGFIASLENRVEQRTLELAQRTNELEKLTEQSKKRADELQIIAEISGYISAEK
                     DLESLLPLITKTVSERFGFYHVGIFLLNENGKFAVLRAANSPGGQVMLKRQHKLEVGQ
                     VGIVGNVTATGIARIALDTGADSTFFNNPDLPETHSEMALPLIARGTIIGALDVQSTF
                     PNAFTQNDISILGLLADQIAIAIDNVRLLDETKKALAEAQSSFREYLAGAWQNKSASE
                     IFGYYQTLTGGQLITGEKTPEVVAKTEKDENALSIPIQLRDQVIGTVHIRPNKEDRAW
                     SRDEINIVNAVAERLGLALDNARLFEETSSRASRERLVTEITTKIRGTNDPQEMIRTA
                     VQELQRALGASRVEIVPRKNAPPPDK"
     gene            33118..33906
                     /locus_tag="PLE39_01750"
     CDS             33118..33906
                     /locus_tag="PLE39_01750"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNE67074.1"
                     /translation="MIKKIAISNEKGGVAKTTSTISLGAALVDMGNRVLLVDLDAQAN
                     LTLALGLEPGESAITSSEVILDNIPLLNALRKTEIENLDLIPSNSRIETAEQFLPVRT
                     SYTTTLQRAIASAPNLHYDYILFDCPPALGAITQNALIAADLLIIPTQAEYFSAYALR
                     NMMTLIRRIREGENPNLAYRVLVTMLDKRNRTHRNIHDQLRSTFGEGVFSTVIETDTK
                     LRESPIAGLPISHYRPGARGSIQYRVLAQELIEYAKETENRQTA"
     gene            33872..38245
                     /locus_tag="PLE39_01755"
     CDS             33872..38245
                     /locus_tag="PLE39_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67075.1"
                     /translation="MPKKPKIDKRLNKLFKDINLEEASSSLKQEAKDEAESKASVTET
                     APASKPDPKPASKKTDTRPIPRQGRIHTSTLIPPEPVITEEHENIASTYAVNIQTGYQ
                     DWATLRILDETTQRQWSQDEELLVKQVTDQLSLALENAQLFQETQKRANELATLNEIV
                     QAISEQIELKNVLNTAYEQIVKLIPLDAFFVALYDESSNTVSYPIVIDEGKSYEEPNE
                     ALNPENFTGRVIKSGKLLLEAVKPEDLKSKSINSDSFGNKNKNYGTMLFLPLRSVNKT
                     MGCLSIQNYDYFAYKQEDIALLENIANQISIGIQNAQLFNDAKQRNEELAVLNGLSGE
                     LSTLVGINNVAESVFKYTSKLMRTSSFFLCLLDEKQMEVSFPLVIDDGEKVEVAPRKA
                     GGNGLTDYVMRTNQTLFLPNRVNERSLALGINITIIGNPTSAKSWLGVPLALGNRILG
                     AIVVQDPEEENVYTTHQRDVLITIAQQAAVAIENTRLFQETEKRADELSLINRVVSTL
                     TGATNLNNTLGLVIRELVEAFPVSRGAIALLNDEKQSFIVVAEHSKQDNHNSALGLEI
                     PIKGNTSTESIISTRKPYILEDAANNPANPPEIREEFQRLGIVYSAIFPIIVNDTVIG
                     SLGVDTTQKDTRLDPNEITLIETTLSPISATISRLNAEEALSRSEADLRTLFSSMEDV
                     VLVFDADGRYIRIAPTNPGRLVRPPEDLLGKLMHEVLPANVADQSVAAIRHTLETGET
                     NQLEYELQIGEEKFWFLANLTKLKDDQVFWVARDITDRKKAEESIRRRNAYLAASSEI
                     GKIVTSTLDLNTIFARTVNLINERFGFFHASIFIVEETGFNALLRESTGAAGAEMKKN
                     KHSLPVNDKSIVGKVALDGQAIVVNDVKIDSLHKFNPLLPETAAEAAIPLRIGNRVIG
                     VIDVQSKSTNSFTDDEISVLQTVADQVAVAIDNARSFELSQQAVMEMREIDRLKSQFL
                     ANMSHELRTPLNSIIGFSRVIIKGIDGPVTELQQQDLTAIYNSGQHLLGLINDILDLS
                     KIEAGKMELAFDEVNIVDITSSVLSTMSGLIKDKPIQMKRILEPNLPTVRADAIRIRQ
                     VMINLLSNAAKFTDEGDILVEVGLKPGQGSTRRELRVSVTDTGPGISVQDQAKLFQPF
                     SQVDDSPTRKTGGTGLGLSICQHIVNMHGGRIWIESEVGKGSTFHFTLPLYRKEEEDA
                     DSGRKTVLTIDDDPQVIGLYERYLQQQGYQVVPLSDPSMAVERAKQVKPFAITLDVMM
                     PGIDGWQVLDKLKADPETRNIPVIVCSIIEELEKGFNLGATDYLVKPILEDDLVNALD
                     RLNSDGSIREVLVIDDDPDDLRLIGKILTDDGRYKASLAEGGRMGWDIISSGNPPHAV
                     ILDLFMEEMDGFRILENMQASEKLRNIPVIIVSGMDLSAEQKEKLNESGQRLLTKGTF
                     SEKELLISIQRSLEKAQAKNK"
     gene            38260..39912
                     /locus_tag="PLE39_01760"
     CDS             38260..39912
                     /locus_tag="PLE39_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNE67076.1"
                     /translation="MSFIIKCLDCGHNAPYYPTSTNCPKCNSQWREAEYDYELIGKTL
                     PAKLAGRPFDLWRYRELLPVRNPNVSLSLGEGGSPLIRAVNLGMMLGCPNIYIKDERQ
                     GPTSSFKDRQASVTIAALKEAGITEMVAASTGNVAISYSAYASRAGMKLWAFVTSLVP
                     ATKMREIALYGSQVIKITGSYDQCKQVASEFARQRHLYQDMGARTITSIEAMKTIAFE
                     IAEQLTAIQGTDGAAPWRTPDWYVQAISGGMGPIGVYKGFREMKDMGWVNRIPAFAPI
                     QAEGCAPMVDSWKKGLEKAEPVVSPKTRIETLATGDPGRAYEFLRSYVNGTNGAFESV
                     SDEDAYRAMHVLAKMEGISAEPAAAVAFAGLFKLIRAGIIKPSDTVVVNCTGHTMPAE
                     PIILGENWSRDVKLPTSQETPQEGLLAALTQVAPERFPKIAIVEDTTEARRLIRRILQ
                     SQGNFTILEAGNGREGLELIQRELPDLIILDLMMPEMDGFTVIEALRAKQETATIPVI
                     VATAKELTPDEKNRLSGQIQSLMQKGDFLNDEFLEEVKTLIK"
     gene            39941..40888
                     /locus_tag="PLE39_01765"
     CDS             39941..40888
                     /locus_tag="PLE39_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE67077.1"
                     /translation="MQKKHTAGVLAALSSAVFLGLAPVLGKLAITLGFSPFAVVALRT
                     GFAAGILLISITLFSPQYLYIFPVGLVGCMFAGAINGLGSLLYYLALQRLHANVGQLL
                     YSLYPFFVALWLILDHQAPSRLTLYRISLATAAVALLTGGPQQQIDIVGVFMMIGAAI
                     LYAMHLPINQRVLYEVPAPTVALYTLLSMSAVVVPVYLIFDRAWPIGEVPWLPVIGLT
                     FATVFSRIALFLGVKKIGGMQTALLGLAELLIAIIFSDIWLGENLSLLQWLGAAGLGL
                     SLILVMYEDTNPPTYSKRTGWLSWIRAPDLPKDIFGPYE"
     gene            complement(41032..41763)
                     /locus_tag="PLE39_01770"
     CDS             complement(41032..41763)
                     /locus_tag="PLE39_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE67078.1"
                     /translation="MKSNILVVDDEPVARQSLADILKLEGHNVTAAPNGQAAVEYVRT
                     HPIDLMIVDLRMPGMDGLEVVQVVNQISPETEVILLTAFGSTETAIQALRLRIHDYLL
                     KPASPAQVLASVKKGLARRTAKSRTSNVTPADVDEAMSVFSLKDGTTVDLSRRQIKHK
                     NKIEHLTPAEGRLLRILIENEGKVFSHRELVLLVQGYDTSQREAPEILRPLVSRLRHK
                     LDQFPSLSERVVSVRGTGYLYEGNK"
     gene            complement(41760..43340)
                     /locus_tag="PLE39_01775"
     CDS             complement(41760..43340)
                     /locus_tag="PLE39_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE67079.1"
                     /translation="METPFVFVVDDEPGIAMLCKRLLSRSGYTVESETDPRKAINYLK
                     ENKIDLLLVDIRMPEVDGFEVIQHAQRLQPDAAMLIMTGHGTVETAIRALRQGVDGLL
                     LKPFSKGSDLVDAVKLALADSQQKRDAARTQALRPLFSVTESLLSETRRDPLLDLILS
                     AICGHLQCSHAACYQQNNETHEFTLVASRGREISKNHLNLISHIDESATPLLLNASGP
                     GNTTLRSLLTDLRLGAAILTPIQRPNLRMVLYAARNLNEPPFRESDLELFQILARQAA
                     VALENSRLYAEQLDYVRKVEDSQKALLQAEKMAAAGRLSASIAHEVNTPLQAVQNCLH
                     LAGREDLPAEKRQEYFNLARTELERLTVTVRRMLDFYRPGASSLETVDLVEMLQYILN
                     LMSKQLSEANVKVVVDFFGKISPIQAVGSQIQQVFINLILNAVDAMPEGGVLKIIARQ
                     SKGGVELLFQDQGKGIPEEKQSNIFEPFFSTKDGGTGLGLTVSYNIITAHGGVLELLP
                     ERGPGACFRIFLPIGGEQ"
     gene            complement(43343..45949)
                     /locus_tag="PLE39_01780"
     CDS             complement(43343..45949)
                     /locus_tag="PLE39_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67080.1"
                     /translation="MQTEISLESQLKDAAQLSEAEWAVLAERVGGTWLLRGVFHLNRS
                     AQTELAAIMARPSIDAWMCGALSGGHPRSSSIPEGSGLAGSRFFVFPVTGSSRAVLVG
                     ADKQSPTAQRIWKLTASLLSNHSSLPDQPFLPDLQSGLASDLPLAIEKVLNTIVQTVN
                     CQGAWLAIRRGESLDIQAEWNDSKAKNLSLSIDTNPILRRVNRSLSDLVVSRTHPNWD
                     AIPCGPLKPNTRIWICLPLVIGQRLIGVVALWRQTEFSSSELGQLRDLAQRVSPSVEV
                     LVTFSEMAAHLRRLGLLNDFVLTVSSAQSLDQVARRMFGLLARAFNTELIALFLRSSD
                     GRVLRDYRMVDGKLNVISVSLAGHSIQPILKEARLRRVTDAIKEGFVPVFKGARSALI
                     VPLKYRGQAIGVLIIENLHAEAYSQYDEHLMVVITSHMAGLIEYTRLREEAEGRARSL
                     GLIHEVVQQVIGLNDKKEVAHIAAELVAQYFKYELAAILLVDEEGSFSIQGIGGTQAA
                     VVQKALADQGNGEYIVREGVTGLVSQSGESILINDTRQEKLYKPLKGWEAHSEICVAL
                     KDGENILGIIDVESREQNAFSHNDLIAMESLAGILASVISSANQYQKLQETIRQLRAI
                     EIELNSRIEAQRSAENRLIQAAKLAAVGEMAAGIAHELNNPLTTVTGFSELVLEELPE
                     GSSHRDDLEMVLREARRASDVVHRLLDFSRQGERIRTRADLNEVVNDVIALTRHLIQT
                     NGVNLTLGLSSSLPWVSIDRNQMKQVLLNLIHNALQAMPKGGDLFVSTDSAVRAERDW
                     VIMAVKDSGIGILPANQDRIFEPFFTTKGDSGGTGLGLSVTYGIVTDHGGSIDIASEP
                     GKGSTFTVWLPI"
     gene            complement(46063..46257)
                     /locus_tag="PLE39_01785"
     CDS             complement(46063..46257)
                     /locus_tag="PLE39_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67081.1"
                     /translation="MNKAEGRDYSAPKDESLQAFKDWILGIYFNLTGKKTDDMSEEQW
                     VAKWREFWQKQKDKQEKNNN"
     gene            complement(46250..46897)
                     /locus_tag="PLE39_01790"
     CDS             complement(46250..46897)
                     /locus_tag="PLE39_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE67082.1"
                     /translation="METKQEYLLVVEDIPDILKLLEATLVFKGYRVVTASNGQEALEV
                     IQKERPAVVITDILMPKMDGFSLVHRLRINPETRDIPVVFLSATYVAPEDKTFALTIG
                     ATRFIEKPVALEEFLPVVEELLKKGAPTAATKLPNEFEFYDGYRKRLETKLNHKLTQI
                     TRDEHLVRTLSGAEKVSIETSLRHAIEERKEIELLLGQIRAQLEKLSKPERSADE"
     gene            complement(46959..48305)
                     /locus_tag="PLE39_01795"
     CDS             complement(46959..48305)
                     /locus_tag="PLE39_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67083.1"
                     /translation="MKSRPVYKAWGCWKVLFYLLFAACSPTPTTSPLENITVTATVPV
                     EVAVTPFETESIVSAVQIRQSEVELITDAAATGDGGNIWGGHQTRVVRTDAGVFTAYT
                     VSGKDELHRVWRLVRRNAVNDWVVVAEGDAGREPVNLLAAPDGTLYLVGWPEGVATLW
                     AGKPIDGSFAMEAQVIPNMIQGNWPYSSAGIASDGTLCIVASNGGEEPGGEFYWSCYL
                     PADRQWVSRISELDFRYCYTYVFPQADGQFALVSTRDVRWQALGYEQPADSFDYAFNA
                     IGFWRTDDLLNKPLELISFDEEPPTAAYPLPFLNAQMDAYLDTHGNMHILYWREGQTT
                     GGERWVMHRILSQMGEILFDERLPEDSGYFNRIFQDSHGRFYILGSSGLLYPLDENGE
                     HPTKPIRIDLGGHEVEYSGYGLSVPRTGSALVNTIDVVFPSGNETQWLYFQIEFSE"
     gene            complement(48302..48802)
                     /locus_tag="PLE39_01800"
     CDS             complement(48302..48802)
                     /locus_tag="PLE39_01800"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNE67084.1"
                     /translation="MRFGRTFYIAVLLLCLFEMARLWLITPDQMAAHFNAQGLPDRFA
                     PKAEFFWFQAQTLLVVILVSLPLQVLFLVLPPNLINMPNREFWLAPERREETIGRLNS
                     FGAMLFGVIALMIQTAFEISAYANLRTPIQFNAGLMGAIMVTGMVVIGLLLMQLLISF
                     RLPRTK"
     gene            complement(48832..49137)
                     /locus_tag="PLE39_01805"
     CDS             complement(48832..49137)
                     /locus_tag="PLE39_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67085.1"
                     /translation="MNYWYSLNLGDGIMAGVPSAEIEAFFQRAFISAGKPPEMAVFTR
                     SESEGRLHCEVIAYFSPAAAEVALAFQAHACAQPARAGLGLLAGDEHSWSLLFPEGQ"
     gene            complement(49159..49515)
                     /locus_tag="PLE39_01810"
     CDS             complement(49159..49515)
                     /locus_tag="PLE39_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67086.1"
                     /translation="MAFRVRVVGVPVQDGSAVWVVDYVLWWPVDDYIGRNARPEFRES
                     VDSSLYLDHVGIFNKEEFIRLNDEYKASFLANPAKKQIHAEQQKRMDVIDQHLKKHLD
                     TKWILVEQYEWESGMN"
BASE COUNT        13660 a        12712 c        11269 g        12035 t
ORIGIN      
        1 aggcagcatt gaactcgaaa agaagcaagg ctccgtccct ggctttggcg atggtggcat
       61 gggcggcgcg aacgacgaaa aagacctgct ctcgcacatc atcaccaccc tgaatgaaac
      121 ctacggcttg aacctctccg aagacgacaa agtggacatg caaaccatcc gcacccgcct
      181 cgaagccgac gaagccctgc aagccgtctt atccgctcaa aacactcgtg ccgacaaagt
      241 gtataaattc aacgaagtgt tggataggtt gatactagaa tttgtacatg gcaaactgga
      301 tttatacaag aaactgaccg agccaaaggt caatgacatg ttaaagcgtc aatggtttga
      361 aaagtttgtt caggaattcg gcaaagcgta atcaatccga aagcctctca actccaaaaa
      421 cgtgtaaaaa cattgtattg tcaaacagaa tcgacccgtt tataattggg gcatcaaaca
      481 gcgcaagaaa atgaacgctt gcctccccaa atgagccaaa cgggatgcgc tgaaatccat
      541 caaatatgac ggtgtaaccc ctcgaattta gacgcgaatc tcgctaaaaa caagcgtttc
      601 ccgttaaacc aagcgggtgt agttcagtgg tagaacgctt gcttcccaag caagatatcg
      661 tgggttcgag tcccatcacc cgctctaaca agtcgaggat ttgcctgcct cgatttttta
      721 ttccatctgt aaggttgctg ttatgtaggt aggcatgact tacagtaaaa ttcaggtcgg
      781 aggcagtcgt atgataccgg aaacaaaaaa aggtactata ctttacgtgg aagacaaccc
      841 tgataacaga agtttgattc gcagggttct tgaatctgag gattattctg ttatcgaagc
      901 agttaatgca agtcaggcac tcgaaaacct tgaaaaaggc aacattgacc tggtcctgat
      961 ggatattaat atgccagata tggacgggta tacccttaca gcaaagatca aatcagttca
     1021 aaagtttaca aaaatcccca ttgtggcagt cacagcaaat gtgatgcgcg gcgatcgcga
     1081 aaaatcactg gaagcgggat gtgatggtta tattcaaaaa cccattgata ttgatacgct
     1141 tacccagcag atcgagcgct atataacaag gagttctaat gtctgatcaa atccctgcca
     1201 tatcagatac tcaaccgatt cggaaaccaa acatcccaaa agatgcggct gttctggttg
     1261 ttgaggacaa tgtttccaac tttgtgttga ttgcacgcat gctcgggtat ctgggaattc
     1321 attgtgaatg gaagacatca ggctatgaag tggtagagta tgccgacaca ctcccccgcc
     1381 ttgaccttat tcttatggat atccgcctgc cctatgaaga tgggtatggc gcattaaaga
     1441 agattcgcgc ttctgagcgt ttcaaagcgg tgccgatcat tgctgtgacc gcagaagcca
     1501 gtatggagca aatggcaaag gccaaagatt ctggctttga tggctttttg ggtaaaccgc
     1561 ttgaccccga ccgtttcccc gatcaaattc gccgcatttt gagtggagag cctgtttggg
     1621 agtatagtta atttttcaat gacagcgact caaacgacgc ccggattaga ctctaaacaa
     1681 aaaagataca agggcagtct gacaggtact cttgttcgaa cgctcttaat ctttactttc
     1741 atccctctcg ctctcatggc gggaacggct tatttccgta cgcgtacgtt gcttcgtgag
     1801 caggcaaata accaagctca aaacctgttt gtatcacaat taaatatcat caaccgtgaa
     1861 gttggagaca aggaagtcca ttttcaggat ctattggcaa gcagtgattt tcagatcatg
     1921 ctggaactgg cactgcactc cgaccccaaa acggatgaat ttcaggtcct gagagatgga
     1981 attctgcagg agtttagtgc catgaatcgg cacgagaaca tgcctgcatt cgatcaattt
     2041 ctcttactgg atacgaatgg aaacataaaa attgccacag aagaaaagtg gcagggggaa
     2101 acgctcgacc cagcaatcat tggtcaagat tttgatactc aacacaccct ggccctttac
     2161 gggctttcgc ctttatacga ggaggagttg cttcttgtca ccaccctcag gtatgagacg
     2221 aaacagggag ccgttctggg cttcgttgta gggatcacag aaaagaaaag catccaaaat
     2281 tcgatgcagt ccctatccgg cctgccgatg agttcagctt atttggtgct ttccaaccag
     2341 caattcttcc agagccagcc cgaagccaat cggtttacgc tattaacatc tcctcttgaa
     2401 gctcaatcag agcttgaaac tgccttgact gagtccaggc ttgatggcgg cgtctcaccc
     2461 agatccattg aaaccacaac cgcagaaaac aagatcaaag tctttcaagt tcaatgggtt
     2521 cccaaactgc aagtttggat gatccttgaa accgacacga ccgaagcata taaaagcctg
     2581 accagtctgg cgccgtttac tgcaattctt actacgatca ctttattggc catcggcctt
     2641 gcattggcaa tcactattaa tcgcgtcatc aaacccttgc agtcactttc acagatcgcc
     2701 cacagctttg cagacggtga cctgagtaaa cgtgctgaga tcaaaagcga cgatgaggtt
     2761 ggcttgctag ccagttcctt caaccacatg gccgaagagc ttggaagcct gtatcgctca
     2821 ttggagcaaa cagttgacga acgcagtcgt caaatccgca ctgccgcgga agtggcgcaa
     2881 agtgttacaa aactttcgaa cctcgacgaa atgcttgata aaaccgtcga actgcttgtg
     2941 cgtgaatttg gcttctatca agccagtgtt ttcttgatgg acccaggcgg aaaggcgctt
     3001 gagttcaaaa cggggttcgg ttctgcaacc agcgccttga taaaaagaaa atacaggctg
     3061 gaagtaaact cagaatcgat cgtcggttgg gtaagcacca acaatcagcc gcggatcgcc
     3121 tccgacgttt cagaagatcc gatccaccta aaaaacgaat tgctgcctga gacacgttct
     3181 gaagcctgtc ttccaatctc aattggcagt cttgtattgg gtgttctgga tgtacaaagc
     3241 atacaggtgg gcgtttttga cactgaaacg gtcgccatgt tacagacttt ggccagtcag
     3301 atcgctactg ccatccaaac tactggtctg acagagacca gccagatcaa ttttgaagaa
     3361 cttgcccaac tatatcgctc cagcaggtta atagccgaag ccaataccga gaatgaaatc
     3421 ctggaaattg gacgcgacgc ctttgcagat gctccctacc ccgtaattat tctacgggtg
     3481 gtaaataaca agctggagat gttctcacaa accgatggaa aaagcggaaa cagcgtaaaa
     3541 aagatcagcc tgccaagtgc cagcgaggcg tctgttgtgg acattcacaa ctacctgcac
     3601 aacggacaag tgatcatcac ttctgaagac aaaaacattc ccgccgtact cgacagcatt
     3661 atgcaaacac tccgattctc ctccgctgca gtttttccat tgcggagagg tggtctgctg
     3721 catactgtca ttatcatggg tgggcagcaa ttgttttcaa atgcaatcgt acagccatat
     3781 gccaatctgg cagacctcat gtccgtgacc atggaaaagc tggatgcaat ccaacaaacg
     3841 gaaaaacatc tccgtgaagt ggaggcactt gcctcgatca gcgaagtggt cgcaacatcc
     3901 tccgatatgc aaagcttctt catgaatctg catgccaaga tccgacaggt tatcggcgac
     3961 tatgccttta ttgttgcgct atacaatgaa agagataaca cggtcagtat tccattcgcc
     4021 tacgaagatg acaaaatcac ctcaatcgaa tccttcccgc tcggtgaagg cctgacctcc
     4081 atcttgatcc gcactcgcca accgttgatg atcgtggaaa atgccgaaag aagaatgatc
     4141 gaactgggtg caaaattttc aggcaagcca ggacgttcct ggctgggcgc ccccatgttg
     4201 gtgcaaaatc aaccagtcgg cgcgttgatc attcaagata ctgaaaaaga acatgccttc
     4261 gacgaggaag atctgaaatt cttcaacacc attgctggcc aggtcgctgg ggtaatccac
     4321 aatgcccacc ttctggatga aagccagaac aaggcgctcc aattggaaac tgccgctgaa
     4381 attgcacgtg acattagcgg atctctcaac cttgacgaac ttcttatcaa ggccgtcaat
     4441 tttattcgtg aacgtttcga tttttaccac gcatctgtct ttctacatga cctgccaggc
     4501 gagtttgcgg tcatccgcga agccacaggc gaagcagggg ctcagatgaa acgctcaggg
     4561 tataagatcg gcattggttc aaaatcaatc gtcggttatg taagcagccg tggggaacgt
     4621 cttgttgtcg gcgatacgac caaagacgcc acttattatg caaaccccct gttccctgat
     4681 acacggtcgg aagccgcagt gcccctgaag gttggtgaac gtattcttgg cgtggtggat
     4741 gttcaaagca cccgtccata tgcgtttact gaagacaaat tacgcagttt gcagatcctt
     4801 gctgaccaga tcgccattgc cgtggtcaac acagaattgt ttgctgaaac gcaggagcat
     4861 ctctctcagc accgtctttt gcatcacatc accaccactg ccgcttcggg caccaccctt
     4921 gaagaggcgc ttgaaaacgc agtgaacggc cttcaggtta cccttggtgg cgaccgcgtt
     4981 acgatcatgc tggccaaccg cgagaaaaac ctgctcgaag tcaaggcttc catgggctac
     5041 gcagaagata ttgccaaagt acaagttccc attggcagcg gcattacagg ctgggcagct
     5101 gctcacaaac gacctcttag gatcaaagat gtttctgacg atcctcgtta catccgcatt
     5161 agttcaaaca cccgctcaga attggcaatt ccacttgtat accgcaatga actgctcggc
     5221 gttctgaacg ttgaaagcga gcaaacggat gcatacacag aaaacgatga ggaaatgctc
     5281 ggcacacttg gaggtagttt ggcagccatc atcgccaacg cccgtctgtt ggaacaaatt
     5341 cgcattcaag cagaacgaga acgtttgatt tatgaagtga caagcaagat ccgcaggtca
     5401 accgatatcc aatccatttt gaacacgacc gcaagtgaac ttgcaaggct taccggcgca
     5461 caatacacta agattcgtat tcaacccggc aacgacccaa cacaggaagg taattgatga
     5521 tggaaagtta tccttccacc accaacgcaa gcaacagccc ggaagaagcg acacgcctgt
     5581 tgtacaaatc ctggcgtgag cagttcacca tgcctctttt aatcgggtcg ctggtgttgg
     5641 gtatccttgc cttaatccca gctttaagtg gtgcgggcaa cacatttatc agggtcttgt
     5701 ttatctcagt ttacgtattg acaggcattg tcacagttat tcgtttttca tattccatcc
     5761 gcatgagcgt tttcctgaca agcatttttg cgcttgcatt ggcagaacta tttactcatg
     5821 gaattttagg tgacagttta ttcttcttcc ttgggttgat cgtttttgca acccttatgg
     5881 tatctcccaa aacaggcatc gcagcgatca ttgtcaacat tctgactttt gttgcggttg
     5941 gcatatcaat gcagagtggt cgcctcgtac cgatcaatcc aaacgctccc ccttccaaca
     6001 ttagcgactg gttcagcgca ggtgcggcga tcatcctgtt tggtgttgtt attgtgctgg
     6061 gttttcaacg gctcgaagtt gcgatcttcg aaacacaaaa acagatccag tccactctct
     6121 ctgcactcaa acacgaacgt gacaaccttg agaccagcgt ttcagaccgc acccagctct
     6181 tgcaaaaggt caatcgtgtt ggtcgggcca tcacagccat tctagacccc aacgaactcc
     6241 tcagccatgc cgtgtatttg atcagcgacg aactcgaatg ctattacaca gcgatcttta
     6301 ttgtcgatgc aaccaaccaa tgggctgaat taagagccgc caccggtgac gccggcaggg
     6361 ttttgattga aaacaagcat catttgaatg tcaacgggaa aagcattgtc agcacggtgc
     6421 tgcggacaaa acaggcccgt gtcgcactgg acactggagc ggatcctgtc cgatcagata
     6481 atcctcttct cccctatacc cgctcccaaa ttgcgctgcc gctgaccatc ggcgatcgtg
     6541 tcatcggggc gcttgagcta cactccacaa aagaaaatgc tttcatgcag caagatgtcg
     6601 atgcctatca aaacatggca aaccaaatca cgattgcgat cgaaaattcc cgcctgttta
     6661 ctgaagcgca acaaagcctt gccgagatga gtgccaccca acagcaatac ctgaaaggtg
     6721 cctggctgtc gctatcatcc gaacaaaaac tcgaatatgc cgtaggcgat agcgaatcca
     6781 gtgataaaca ggaaatcgaa gttccattga cactgcgtga tcaggtaatc ggtcagatca
     6841 gcctatccag cgcaagtgac tggacctctg aacaaaagaa tttgatcgaa tctgtggcag
     6901 tacaagctgc ccttgctctt gaaaacgcgc gcctggtgga agaaagccaa tctgttgcaa
     6961 cacgtgaaag gctgatcaac gagatcacct caaaagtatg ggcctccacc acgattgaca
     7021 gcatcctcca aaccacagtg agagaactgg ggcgcgcact ggaagctgca gaagtagaca
     7081 tagaagtgac catggatgat gacaatgaat agcaaaacac caatcaccac ctcttctgcg
     7141 ttcgattaca taggctggcg cgaacagttc atcattactg ttctgcgtat tgcctgcgtc
     7201 ctgggtgcgg ctttgatcgc aacctccttt ccaactgcat cagccactga tcgtgttttg
     7261 tttccgagcc tttatctaat tctcctcgcg atcacggttc taaaaacccc atattctgtc
     7321 cgcgcagcca gtttgctttt catggtttat attattggga caaactcgct cctttcgtgg
     7381 ggtccctggc tggatggcag catattcttt atcgcattta ccatccttgc tgccctgctc
     7441 ttcgacaatc gcattgactt gatcgctctc atcctcagtg cattaacctt tgtaacactg
     7501 ggcgtactac agcaattggg catttatgaa ccttcagcaa agggtgtccc ccccaccatt
     7561 gcaacggatt gggccgctta tacggtcgat ttttccatcg ttagtgcaat catccttgta
     7621 gctacaaatc aatttaagaa ggcgttgacc caggtcattt tcgaaatgca gagcgcgttc
     7681 aattctttaa cgactgaacg tgaaaaactg gaagacaggg tccgtgaaag aacagaagaa
     7741 ctcgaagcac gggcaattca attgcgcgca gcaacaagcg taactcgaac cattgcgggc
     7801 attcaggata tttctgagtt attgtcggaa accacccgcc tgataacaga tgagttcggc
     7861 tactatcatt caggcttata tcttttggac gaacgcaaaa ggacgacgtt tttacaggcc
     7921 tcatcttccg ctacaggaaa gagcctgatc ggtcagggct tccgtatcga accaaataaa
     7981 ttaaacatga ccaatctggt tattgagcaa agacgcccgc aaatcgcaat ggatgtggga
     8041 gacgccaact tcatccgtga ccccaatttt ccgttgaccc gatcgcgtat catgctgcca
     8101 ctcacggtcc gcggacaggt gattggtatt ctggatatcc actccgatca accacggaca
     8161 ttcgatggac aggatgcaga ggtgcttcaa actcttgcag atctcgttgc catttccatt
     8221 gacaatgtcc gcctgatcga tgagacaaaa agcctggttt atgagcttga atccaacact
     8281 actctgcaaa cccgtgagac ttggtcaaaa tttaccagtc gccacaaacc tgcatatcaa
     8341 tatactcctg cgggtgtacg tccgcttttt tcaaaaatta aaaccgagaa ggcagacggg
     8401 cttcatgtcc ccatgatcct ccatggacaa aacataggca acatcgtcct aaagagaaag
     8461 ggcatctcgt caacgtggtc tgaacgtgaa cgtatcctgg ttgaaaaagt ggccgctcaa
     8521 gtagcgcttg cattggaaaa cagccgcctg gtggatgaga ctcaaaagaa cgcgatgcga
     8581 aatcaggtca tcgcaaatat atcctcccgc gtccgcgaaa ccctcgacgt agaatctgtg
     8641 gttcgcactg caaccactga acttcgccga gtctttgacc tcaaggaagc tgaaatcagc
     8701 attggtgcgc cacaacctga gccaacccag cttcgtaaaa acacaagttc acttcgtttg
     8761 aaataatatt tatcagtctc tcaaggaaag ctaaatgcgt actcgcagcc tacccacatc
     8821 caccaagtca ttgaacaaac ctccacggag aggtttattt ggtcgctatg tgagtctgca
     8881 agttaaactc cctgtagtgg tgatcaccct gctcttgctg gccttgtttg catctgccta
     8941 ccttagcatt caagccgcaa aaacagcttt gatcgacacg ctgaaaaatg agcttattct
     9001 tcaagctgac tctaaagcag agctcattcg ttccaacctg atttggacga gggctgcggc
     9061 aattgatctg gctgcatcag cagaatcagt gaattacgac gaagagacca tccaaaaagc
     9121 cattcgcaac acgcttctac gcaacgaaca ggtttttggt tccaccattg cgtatgaacc
     9181 ttttcaattc cattcaaatt cttattattg ggcgccatat tacagccgtg ccgcaaatgg
     9241 agagttgcaa ttcacccaac tcggcaatcc cgaatacaac tattttggct gggaatggta
     9301 cacacttcca aaagaaaaga atacacccgt cctgtcaccg ccgtattttg atgcaggcgg
     9361 tggtgagata tggatggtga catggagtgc gccattctat aatgaagctg gcgaattcaa
     9421 gggagtggca actgccgaca ttgccttttc acagacacag gacatcgtaa gcaagatcga
     9481 agtaggcaaa aatgggtatg ccttcctgct aagccctgaa ggtgtgattc ttggcatcgg
     9541 ttcaaatggc ggacaatatc agaccatggt cgattccatg atcgcggctg cccaatcttc
     9601 ctcacaggca tcgggctggg caagcctggt tacctccatg atccagggca gtactgggtt
     9661 ttccgaagct gtggatccac agggccgttc gatgttcatt gcctactctc ccattggttt
     9721 ggatacgggc tggtctcttg ggttggcatt cccacaggcc gaattattcc aaagagccgt
     9781 acagcttcaa aacacgcttg gggtttacac cctgatcatc gccgttattt ttggcgtgtt
     9841 cttatttgta tataccctgt ccataaccaa cccgcttcgg cgtttggcct ggtatgtcgg
     9901 ccatttttca cctgaaaaga tcaaccaaaa cctggaacct gtccaaattt acacacgtga
     9961 tgaattggaa gatctatcca ccacatttca gcaaatcacc acaaaccttt tcaacacatt
    10021 caccaacttt gaagaacgca ttgccgaacg cacaagagaa acagaacgcc gcagcatctt
    10081 gttgaaagct gtggcagatg taggcaaagc catcacgtct ttccgtgacc tttccgaact
    10141 tctgcagcaa accacatatc tcattaacga aaacttcggc tattatcaca tcggcatctt
    10201 tcttcttgat gaacacaagg aatacgccgt attgagcgca tccaacagtg aaggcggacg
    10261 ccgcatgctg gaaaaaggac accaactcaa agtcggcgaa acaggcatcg ttggatacgt
    10321 gacacaaaat gcaaaggccc gtatcgctct ggacgttgga aaggatgcgg tttactttga
    10381 caatcccgac cttccagaca cccgctctga aatggccctg cccctcatta tcggtggaca
    10441 gatccttggc gcgttggacg tgcaaagcac ggaggctcag gcattttcag atgaagacag
    10501 tgccacactg caaattttgg ctgaacaatt ggctgtagcc attcaaaatg cgaacctgtt
    10561 caacgagacc caaaaggccc tcgaagcttc cagggcggct tacagtgaat taagccgcga
    10621 agcatggacc aaaattcttc gcaaccagcc ccgcattggc tttattgcca cccccccaac
    10681 caccacgcgc acgtattccg acaccgtgga acccagtatt gcaaaggcct ttgatacagg
    10741 cgatattatt attggcagtg acaacctgac catcagcatt cctgtaaaag tacgcggaca
    10801 aaccattggt gccatgcgcc tcaaaaaagc cgaaattgcc gaagcatgga ctcaagaaga
    10861 agtcaacctc gccatttcat tatcagacca ggtaagtggt gcgctagaaa gcgcccgcct
    10921 ctaccgtgat tcacaacaga ctggcgcacg tgagtcgctg gtttctgata tatcttcacg
    10981 catcagtgcc gtctccagca cagatgcgat tctacgcgaa accgtacagg aacttgggca
    11041 ggtgctgggt gacgcttcga tcaccttcca attgctgaat cagcccgatg gacagaagca
    11101 gaccgaggtt gagttaagaa gcgggttgtc aggtcaggct ggaaaggcaa aagagtagag
    11161 tcatgtccat ttcaaagcca ccgctaaaca ctcaaagtgc ttcccgattt gaacgcatcc
    11221 aacagatcgg gcaaagcctg attgcgcccg ccccatcgat caaggacatc ggtcaatttc
    11281 gccgtgccca gctgcttgcg atccttaccc tgatcacaag tgttctcttc tttgccgcaa
    11341 ttttctccgg gccacgcagt tatggcgtat tcattgctct aacattgatt tcgctggcat
    11401 cctatgccct gagccgaaca acaagtttca ggacaggaac ctacctgttc acttatgcat
    11461 ttacagcgat cggcttcatt cgaatttacc agggcgaggc tacttcaatt gaggcctcta
    11521 ttgtttcaac cgtacatgta gctcttgtag tttcgagtgt actgctttca caaagagggt
    11581 ttcttgggct tgttgcgctt tccacaattg caacctttac cgctcctttt tatgcagatg
    11641 cgaccattgc cgcgaactca gcacaggcat ttaccagtga tatcagccga acaggcggta
    11701 ttgtttttac actgggtata ttgttgtatg gcattcaagt cttccgcgcc aatttggata
    11761 aggaaaagct caaagaactg ctcgacacca accaggaact ggaagaagta aaaaagaatc
    11821 ttgagcaaag gatcagcagc cgaaccctcg aacttgaaaa ggccaatcaa caggtacagg
    11881 aacgggctgc ccgttttcaa atcgtttctg agatctcaca agagatttca gcaaatgtag
    11941 agcaaaagcc caaagacctg ctccaatcca ttgcaaggtc aatcagtgaa aaactcggct
    12001 tctatcatgt gggcattttc ctgctcgata agaatcgtga gtttgccgtg ttgcgtgcct
    12061 cgaacagtca gggcgggttg cgcatgatgg atcgtcatca ccaattgaag gttggcggtg
    12121 cgggtattgt aggctatgtt gcacaggcgg cacgtccgcg cattgcgttg gatacgggta
    12181 cggatgctgt cttcttcaac aaccccgacc ttccaaaaac acgttcagaa attgcgctcc
    12241 cgctcaaata tggctccagg ttgatcggtg tcctcgatgt gcaaagcacg caacaatttg
    12301 ccttcaacga tgaagatgcc aatctattaa gtacgcttgc caaccagata tccatcgtga
    12361 tcaacagcat cattgccgct gaaaatggag agcttgggta ttcacaaggc tactttcagg
    12421 ctgaaaaact ggccaggaaa aaagacaccc aggctggatt ttcctacctg ccagatggca
    12481 gcattgtgac agcccgcccg ttaaacgacc ttgccacaga acaggcactc gcatcaggcg
    12541 agaccgtggt actgtcccag tcttcgagga aggttcctcc aacccttgcc attcccgtca
    12601 aattccgcga tcaggtgatc ggcgtcattc acattcaagc aacggaaaaa aaccgcaagt
    12661 ggactgaaga tgaggtcgca ttggttcaat ccatttccga ccgcgccgca cttgcattgg
    12721 aaaacgcccg cctgttcgag gaaaccgtcc gtcgtgccga tcaagaagaa accattgcaa
    12781 aagtcaccac ccagatcggc gcctctacag atttcaaccg cattctgcaa accactattc
    12841 aggaactcgg gcaggtactt ggtgtatcgc ggtccttcat ccaaatggga ccgttaaaga
    12901 acgaagaaga acaataacat tctggcattg ataaaggcag acatgagtca gaacacaccg
    12961 gcacaaacaa ccaaaacctt cacaggaaga gaaaacaacc tgtcgctgca aacgcgattg
    13021 gtgcttttcg tgctgttcat tgctcttgtc ccgctgacca tcatcgccgt acgcgataac
    13081 ctccaaaccc agcaagcgtt gacgaacagc gctgaaatct cactgcaatc cagcgctgaa
    13141 caaaccgcca acagcctcga cactttcatt caaaccacct tgaattcaat tgtgatcgag
    13201 tcgcaattct ctgttttcac agaatatctg tcactgccaa attcacagcg catcacaggc
    13261 aggccagaac aattccgcgc ccagggtttg ctcaataaac taagctcaaa agatagtcag
    13321 aacattatct cttatgccat tctggatgtt gatggcaata tcctgctgga ttcagcgccg
    13381 caacgcgcca aaaagaatga atctgacgaa gcgtattttc cacaggtacg atttagcaca
    13441 caacctgtca ttacagaagt tgcatacgac gaaaatggca ataagaccat cacgttcgca
    13501 aacagagtgc tgagctcaaa cggcacctat ctcggaattt tgcgggtgaa atattcctcc
    13561 aatgttttgc aaaacatcat tgtagacagt tcgcaaacat cagcaaatgc cctcgtattg
    13621 ctgctagacg aggtatatat tcgtcttgca gacagccgta actctggcct catccaaaaa
    13681 tccattgcgc cgctttcccc tgtggattat ttgcaggcag tcgaatcaaa acgctttttg
    13741 aatacatccc ctgaagagca atcaacgaac ttcgtagaat tcgaaaacgc actggataac
    13801 gcatcagtga caccattctt cagggttgat atcacaccca acattcctgg tgatgacact
    13861 gtagccgtgg cgccgatgaa gactcgtccc tgggtcgtca cctacagccg tccaacatcc
    13921 ctgttccttg ctgatgttca aacacaaacc cgcgcgaaca cggccctcgt cattggcgcg
    13981 ttgatcctga tttccattct caccacagtg gtggcgagaa atctaactgc gccgatcgtt
    14041 gcactaacca aaactgcaaa cttgatttca caaggcgacc ttagcgcccg agcagagatc
    14101 aatacaaccg atgaaatcgg ccagctcgcc tccgccttca actccatgac aaaccagcta
    14161 agcaacagtc tttcaggttt ggaggaacgc gtcttcgaac gtacagccga tattcaaagg
    14221 cgtaacctcg aattggaaac catttcagat gttgcccgtg aaatctcaac catccgtgat
    14281 ctggacacat tgttaaatgt atccacaagc ctgatccgcg agaaattcaa tttctatcat
    14341 gtcggcattt tcctggtgga tgagcgcggc gaatatgcca ttctgcgggc agcaagcagc
    14401 gtcgctgctg aagagcttct tgcaagaaaa tacaagctac gggtaggtca gaccggtctt
    14461 gttggcaacg ttacaagtac gggtcaggca tatatcgccc tcgatgtcgg tcgagatgct
    14521 gtgcattttg aaaatccatt cctgccgaat acccgttccg aaatcgtgct gcctcttcgc
    14581 aattacaatg tcacaatcgg cgcactggat attcaggcag atgttccaac cgcgtttgat
    14641 gaacgtgaca tgcaaacttt ccagatcctt gccgaccagt tggccgcagc aattgaaaac
    14701 gcgcaattgg cacaccaggt agctgaaaat ctggctgcgc taaccaacgc aaaccgtttc
    14761 caaacacagc aaacctggcg tacggtcatc gaacagcaaa ggcattcagc atacgaatac
    14821 gatggcttgc agatcaaggc tgtgcctcag gatcttcctg cggatatcat gaaacaactt
    14881 gaaaacggga acccgatcgt atatcaacaa cccacccaag gcagggatgt caaacacatc
    14941 ctcatggttc cattaatagt gttcaaccaa gtcatcggtg tgatcggcct cgaacaggaa
    15001 aaagcggatc ataactggac cgatgaagat gtgaccatcg cccaggcagc agccaaccgt
    15061 gcagcactga ccctggaaaa tgcacggttg cttgaagaaa gtcaacgacg cgcgatcaag
    15121 gaacgcgcca tttttgaggc gacctcccgc attggttcgg ccctaagcgt ggaaaacatc
    15181 cttcaagcca ccgccgagga actggaacgc gtcatcagcg gatcggagat cattttgcaa
    15241 ttcaataccg aaggctcttc tggcataaat gataaggacg aactttaggt aaaaacacgg
    15301 caaacgccac ggatttattg aatcatgaat caaccttctg aaaaccttga tttttcctac
    15361 acaagatggc aagcctcatt tctgcaagcc acattaattg gcgcctgcgt atttgggttg
    15421 ttggcgatca ttccagccgt cctcggaacg gccgatccca ttcttatcgg tatttatatc
    15481 ggcgcatata tcgcactgct actgatcacc atcctgcctg caccatacac gattaaagca
    15541 gtgacacttg taggtttatt gtttctgctt ggcatctccg gtcttttgga aacggggatc
    15601 cgtggagatg cccgcctttt catgcttgcc gccgttacca tggccaccct tttattatca
    15661 tggcgggcag gctggatcat gacaggtcta tccatggctt cgttcataat cgcaggctgg
    15721 ctgattgtta caggagccat caccatttca agtaaaaacg taactccagg aaattttgat
    15781 acctggataa gtgccatcac agcggcgtta ttgcttgaag ttgtaattgt caacggcatc
    15841 aggctaattc aggtcgagtt tgaatcctcc cgcaatcgtg tccgggagtc atttcagaag
    15901 atcgaagaag aacgaaattt acttgaagat cgtgttaagc aacgcactca ggatttgggc
    15961 cgcattaacc gaattagcga acaccgcgca caaatgtttc aaaccatcgc acaggtaact
    16021 cgagccatta tttccactca aaacctgcaa gacctgcttc ctcaaattgc ccaggtcatc
    16081 agccagcaat tcgggtttta tcatgtgggc atctttttat tggactcaaa caaggaatat
    16141 gctgtgttat ctgccacaaa cagcgaaggc gggcagcgga tgatcaatcg cgatcacaaa
    16201 ttgcggattg gtcagaccgg tatcgtgggt aacgtggcag gaacgggaaa accccgcatc
    16261 gcgctggaca ccggactgga tgccactttc ttcaacaacc ctgaccttcc agaaacccgc
    16321 tctgagatcg ctctccctct tttccgaagc ggccaggaac taattggcgt tctcgatgtg
    16381 caaagcacgg aatcaaatgc ctttacacag gatgacatcc agatattaac cacattggca
    16441 gatcaggtat ccatagccat tgccaatgcc cgttcctatg aagaaacaca aaccgttctg
    16501 cttgagtcgc aattgttata tcgccgcaat attcaagcag gctggagtaa attcgtccgt
    16561 tctcaaaaac tttctggaat ccgcaaacag ggcttgaaat caagtctgct ttttgacccc
    16621 atggaagtac ctggtgcaaa agaagttgtg cgctccggaa cgatctacaa aataagtgaa
    16681 gatgaccaaa atacctcgtt aacgattccc atgaaattac gaggcgaagt tgttggtctt
    16741 ttaaacatca aaacagagga tgaaaatgcc atcggcaccg atgaactgga catcatcaac
    16801 gccattgtag agcgtgcggc attgtcaatc gacaacgccc gcttgctggc agaaagccga
    16861 aagacagctg aaaaggaacg cgtgatcgga gaaatctcat ccaaagtaag ctctttcacc
    16921 aaccgcgaca acattttgca ggccgcagtt gcagagatcg ggcgggcgtt gcccggcgct
    16981 gaagttgtca ttcagttgca aaaaaagaac gacacagaca gttaatcgga gagcggcaat
    17041 attatgtcat ccaactggtt cgcagacctc gaagatacca acccgtcatt cctaagaatg
    17101 acgaggaata tcctcgtttt cacgatcatt gccaacctcg ccatcctttt actggttgtt
    17161 ggcttatttt tcagagaatc gcaaaacgca gcctcaagcg ttgcattatc ggtaacgctg
    17221 gttcttgaaa ttgcttcact gatctttgta ctgcgtggaa atccgatccc tgcaaaaatt
    17281 gtagtgcctg tttccctgtt tgttgcggtc acctacacag cgatcaacgc caacggtttg
    17341 cacgacctct caatgctcgg cttcccaaca gtaatcgtag tgagcgcatt gttgttgcga
    17401 aaacgcgccc tgataatctc cacccccatc gccgtactct gtgtggagat tgtggctttt
    17461 gccgacatga ccggtattac gaattcagaa atggcattga agaccgacgt gtcagacgcc
    17521 ttgattgctg gcattttgat gatcgcaatt tcttttctgc ttcaattact gataacccgc
    17581 ctgaatgaaa gcgcagaaga agctcgaaac aatgcagcac tacaggtcga agcgaataaa
    17641 gagctccttt ctctacaagc aaaccttgag aatcgcatcg aaagccgtac aagagagctt
    17701 aatgaagtca acgccttcaa tgaacgccgt gcggcgaact ttgaggccgc ggctcaactt
    17761 gcacgcactg tcgccagcat ccgcgacatc aacaaattgc ttcctgccat cgccgaagtg
    17821 atcagcgccc gttttgacta ctatcatgtt ggaattttcc tgaatgacga cacacaggaa
    17881 tacacgtcat tagtggctgc caacagtgaa atcggtaaac aattaatggc tggagggctg
    17941 caatataaaa ttgggcaggc aggctttgtg ccatctgtag ctcaaactgg gtattcgaga
    18001 gtatctcaag atcacaatca aattaaatct gtttccgatt ctcaggaatt ccaaaaaact
    18061 caatctgaat tggttcttcc catgaaaatc gaaaacaaag tgctcggcgt attggatatg
    18121 catagtgaca aaacctatgc gtttgatgaa gttgcgattg aaattctatc catcctcgct
    18181 gaccagactg caattgccat tcaaaatgca cgtgcatacg aagagacaca ggccgcgctc
    18241 gccgaaagcc agatcttata cggcacagtg attaaacaag catggcagac aaatgtccgt
    18301 gttgagaaca aattgggcta ccgtttttca ggcaccatac ccatgctgct tgaaaaacct
    18361 gttgattcac aagagttaca agctgccttt gaaagtggga aaacggtcat ccttgcatcg
    18421 gaagatcaaa atcgcgcaga tggcagtgtg ctggccgttc ctttgatcct gcgcggtgaa
    18481 acagttggtg tcatccatgt caaaaccccg gcagatatcg acatgggtga agatgaagca
    18541 gatattgtcc gtgctactgc ggaacgtgtg gtgcttgcac ttgaaaactc aactttgctt
    18601 gaggaatcac agaggcgtgc tttacgtgaa caaactattg gccaaatttc ctcaaagatc
    18661 agtgctggca cagaaatcga aatgatctta aaaacagctg ttcgagagtt gggcagtcag
    18721 atcagcggcg cccagatttc ggttgagatt ggaagcgaca atgatgaata gagatacaaa
    18781 catggaacgc gcatccaatt cacagcaggt agatagcgtt ctcgtacgca atgcatatac
    18841 catcagcatc atgctgttga tcacagccat tccaagcgtt ggcttgtttg tttattatgc
    18901 tttgctcaag ggttccgcgc aaatattaac gatatccatc atcatgttca ttgcgagttt
    18961 tggtgcagtc gccgctcttt tcctgatacg ccgcggcaga tcaaatcttg cgatgatgat
    19021 cataatcacc agtttcacca tcacatttgt tgcaatacca tttttcatca aaggccttgg
    19081 tgttatcctt gccataagca tgtttatttt ggcagtgtcc atcacgggcc tggcaatgcc
    19141 tacaagatat gcagcatcag gcttgatcgt cggactctca tctggtgcgc tgatccttct
    19201 gcttgattat ttgttccttg gcgaggcaag agttcgcgtg ccagagctgg agagagccac
    19261 tgttttcatc gtagttggtt tcgcattatt ctttggcgcc ttcacagtcc gccactttaa
    19321 ccgttttagt ttgcgcatca aaatcaccat tagtattctc ttaacaggcg gcgtcattgt
    19381 ggtcaccctg gtttggtttg ccgccagccg tactggaaac atcatcaaca cactcacaac
    19441 caggtacgaa acggcatcca gagaaaacat tcaaaatcag ctgcttcata ctgtggaaga
    19501 cagagccgct caaaccaatg gcttcttcga caaacttcaa aacgacctgg ggattctggc
    19561 aaaataccgc gccagcctcg aacaacaaaa agatatcttt attgacagca catattggga
    19621 tgcggacaat aaagtcatcc aattaacaga aggacaatac ggcaactccc cagcagatgt
    19681 agcctcgata tttatccctt ctacagttgt agtggatcaa tctctttaca atgatttaaa
    19741 tacaacagca tatcttgatt tttatgcccc caatttcctg caaacacact ccagtgtaat
    19801 tgccttgtat ttcattaaca cagcgggtgc caccacctac taccccaaca tcaggcttgc
    19861 ggaaaacgtt cctgcagatt ttgatcccac caaagagatc ttttacacaa ttgcctcccc
    19921 ggaaaacaac ccctcacgtg agccgcgttg gacagatacc tacgaagacc ctgcgggcaa
    19981 tggtttgatc accacacttt ccatccccgt gtatcaaaac gatacattct taggcgtcat
    20041 aggtttggat gttcaactag cccaaattga acgtgccatt gccaatgtaa aggttggaga
    20101 tacagggtat gcattccttg ttgataaagc cggtcatatt ctgtcgatgc cgccagaagg
    20161 atatctgttt tacaatattt cacccgaaga ggtcacaact aattccgtcc atgagctttc
    20221 cgtcctgtcc aagggtcctt tcgatgtaca ggaagcaacc gctcaaatga tcaatggtga
    20281 cagcgagatt gcaaccatca aaaatggaga cggtaattct ttcttcattt tctcacccgt
    20341 tacagtaccc aattaccgcc ttgggatcgt agcacctgaa aacgaattca caacagagtt
    20401 cgttcaatca aggagcgaga tcgaggatat tgttcaatcg gccctgcagg gtgtggcaat
    20461 cattctaatc gtattgcttg ttggcgcaat cattgtcagt ttatggatcg ggcagcttat
    20521 cacccgccca ttggtgcgcc tgacacagac agcagaaaaa atatcgcaag gcgattttgc
    20581 cgcgcgcgca ttagtagaaa caggtgacga aacaggtgta ctcgccaagg cattcaacag
    20641 catggctgag atcttgaatt ccacgctcct tgggcttgaa aaaagcattc tcgagcggac
    20701 tcgggaactt gaggaggcaa accaaagtat cattcgccgc gccggtcagt ttgaagccat
    20761 tgctcgtgta gcccgcacaa tcagttcaac tcaaacgctc aactcactgc tccctcaaat
    20821 cgtggatacg atctcacagg aatttggctt ttaccacgtc ggcatattcc tggtggatgc
    20881 ccaccgcgaa tatgcgattc tcgctgccgc aaatagtgaa ggcggcaaaa gaatgctcaa
    20941 ccgcaaccat agattgctgg ttggcggaac cggtatcgta ggatttgtta ccaactccgg
    21001 acagccgcgc gtcgcgctgg acgttggaat ggatgctgct ttcttcaaca atcctgacct
    21061 tccagatact cattccgaaa ttgcgcttcc cctgctgatc ggtacacaag tcattggcgc
    21121 tttggatgta caaagccgcg agactgatgc tttttcacag gaagatgtca gcatcctatc
    21181 cacacttgct gatcaggtaa gtatcgccat ccaaaatgca cgttcctatc aacagagccg
    21241 tgatgcactc gaccaagccg aagccgcatc catgcaaatg agcgagttgc aatggggccg
    21301 cttcttcgca gaacagcctt caaatggctt tgtatttgac ggcctcgatg caaaagacat
    21361 caagcaagtc aaaaccaagg atgagaacac ctatacaatc cccttgatct tgcgcggaac
    21421 aaagatcggt actcttaagc taaatacaga caatccaaac cgcacatgga ccgacgatga
    21481 gatcgcgatg gcccaagcca ctgccgaacg tacggcattc gccattgaaa acgcccgttt
    21541 gttgcaggaa gcccaaaaac gagcagccaa agagcgtgcc atcggacaaa tcactgcaaa
    21601 gatcggcggt ttgatcaaca tcgaaagcat cctgcaaact tcgatacagg aacttgggaa
    21661 taccattccc aatacagata tcgccatcca aatctcccct gaagagctag agcaaaagta
    21721 aaacgctcag gagaatgata tgttgttaaa gatcattaat tattttagag atgaagaaga
    21781 cagcgaccca tcttttatca gattggtgcg caacattgtc attttcgtat tggttgtaaa
    21841 tatcgcgctt cttccactcg taacaggaat tgtaggagag ggctctcgta acccgcgcgc
    21901 atttttcacg ctcctaacca ccttaattct tgaaatcatc tcactattcc tggttacacg
    21961 cggaaaactg ggcatggcaa aagtggtggt tccatttgca ttaattgctg cggtaaccat
    22021 catctctctg actaccaatg ggttaaagaa cgcagccatg gttggaatgc ctatcattct
    22081 ggtaatttct gccatcctgc ttggacgtcg ctctatttat ctaaccacac ctgtcgccat
    22141 catcgctgta acaatcgttg ctgtcgcaaa tttacaaaat caaatcccta ttatccctgc
    22201 tggcgttgac gatgcgatta ttcttcctgt tttgctattg ggtactgctg ccattgtaca
    22261 gctattgatc acccgcctaa acgatagcat taaacgtgcc cgtgaaagtg aacagatcca
    22321 gaaaacagaa aacgctgagc tgaacgaact gcgttccatg cttgaagaac gcgttaatca
    22381 gcgcacatca gaattggagt tcgcgaatcg gaacaatgaa cgccgtgccc gtcaattcga
    22441 agccatttcg caagtcgtcc gtgtagtttc aagcatccaa gacctggaac tgctcattcc
    22501 tcgtattgcc caagtgatca gcgagcagtt cggtttttat cataccggca tcttcctgct
    22561 tgatgacgaa cgcgaatatg ccatcctccg tgctgcaaac agtgaagggg ggagaaaaat
    22621 gcttgagcgg ggacacaagc ttcaggtcgg tcagaccggt attgtgggtt tcgtcaccgc
    22681 aacaggtcag cctcgcatcg ccctggacgt aggatctgat gctgttttct ttgacaatcc
    22741 tgacctgccc aacacccgct ccgaaattgc actaccactt cgctatgcag gccagatcat
    22801 tggggctttg gacgtacaaa gtactgaagc caatgccttc ggccaaaacg atgtggaggc
    22861 actgactaca cttgctgatc agatttcgat tgcgatcaat aacacacaag tcctggaaga
    22921 atcgcgaaaa gcctttgcag agtcgcaaag cagatttggt gatgccgttc tggaatcatg
    22981 gaagatcatg cagcctgagt ctcttggctt ggggtatcaa ttggtaaatt catcaatcaa
    23041 accattggat gttccactga acggtgatca tattcaagaa gcgatctcac aggaaaagcc
    23101 gatcttagac aatccggaag ggaaagtttc acaacttgcc attcccatcc gtatgcgtgg
    23161 aaaaattata ggaatcatga atctaagttc aaaaaatgat tacgtattaa caaaagatga
    23221 cgctgatatt gccgaagcag tttcagagcg tctttcactt gcaattgaaa cagccacact
    23281 cttaaggaca acccaacacc gcgcagatat cgaacgtgtg acagcagata tttcctcaaa
    23341 gataagctcc tccactcatt tcgacgccat tttgcaaatc accgcccagg aactcagtaa
    23401 agcacttggc gggtcagatg tgctggtaca gatcgagcca gtatcgatgg aattaggcat
    23461 gtccagctag tattatggaa aatcaataat gcgaaccccc atctccaagt tcctgcccgt
    23521 gctgcttctt accgttattt tttctgcctg cacggcaatt tcacagcctg tgtctgcccc
    23581 actgccgcca ctccgcgttg agtttacgaa ttggtggggt gattacacac tgctggtcgc
    23641 acaggaaaaa ggtttctttg aaaaatatgg tgtacaggtg gagcctgtgt actacgagtt
    23701 cttttcagat acctatcctg atctcgccgc aggtcagctg gatgcaggtc tgatcgcagt
    23761 tggagatacc atcaatattt cccgtatcgc cccaatgaaa gtagtcgcag ttcacgacaa
    23821 tggcggagat gatgccatgg tcgcaaggcc tgaaatcctt tcgattgaaa atctacgagg
    23881 cctgaccgtt ggaacccaaa ttggctcaca atacgagttg atgatcacag aaatgcttcg
    23941 cagtgcgaat atggatattg ccgatgttaa ggtcgtttcc atttacccag aagatggttt
    24001 ggcagctctt gaaaacaacc aggtacaggc tgtttatatt tgggagccct atctatcaga
    24061 agcactatca aaaggataca aagtcctgta tcctaaagaa cagttgcatc tcttccccga
    24121 tctgatcgtc tttcaaaaat cggtgacaga ccaaagacca gatgatgtgc gcgcctttct
    24181 aaaagcatgg tttgaagctg ttgactaccg cttgcaaaac ccagaagaga cccgcgccat
    24241 tgccgccaaa tatctgggtg taagtgtgga agaagtgcaa ccagatgaca atctaaaaat
    24301 tcttacattg gaagataata aagccatctt taggcttgaa agtgaaaact ccatctacac
    24361 ggttaccaag atcacatccg atttcctaat cgcaattggg acaatcactc aaatgccaga
    24421 cccgttagaa ttactagact caagatacct gccttaactg tacaaatcaa caagtgagta
    24481 actcatgaat gtaaaaaaca caagcgacca acaagaagaa gcccgtctca aaagaaacgc
    24541 tctaggaatc agcctgatca ccggcatcct ttttcttgtt gtttctgtgt ttgctgtcat
    24601 tgtcggctac caagaaaatg gcatgaacgg cctctggggc ggcgcaatta ctggcttcat
    24661 ttctctggct gcattcttct catccataca ggcctatcgc gggcgtactt caatcggcat
    24721 tgggttctta attttcgcga ttttgctggt ctcctttgcc ataccgatcg ttgcacaagg
    24781 acacggtctt gcactaagca tcctggtcct cgttgtggtg gcaagcattt cctccactac
    24841 cctatcagcc aagtggacga cacgatcaat cataatcgca ttcattgtgg ctgccgccat
    24901 cattctgatc gatctctatc tcccggattt agggctgccg ggaaacccca cttacatcaa
    24961 tatcgtcgct ttggtgacat cacttgctta tatcgccttt attcttagaa gctttaacac
    25021 ttactcacta cggaccaaga ttattacggc atttacacta atcacgatca cccctcttgt
    25081 tactctgggg tatttttaca gcctgtcatc cacccgaatc ctcgaaaacc aaagcaaact
    25141 acaactgacc aatattgcaa agattcaagc tgaaagcgtg gacaacttca tcacgaatca
    25201 aatagacagt gttcacgctg acgcaaaaca gctggctttc gttgaatatc ttcgcctgcc
    25261 agccgccgcc cgttcgggca gcgtaaaaga gttaaatgcg cgtctggcgc ttctggcgtt
    25321 aacacgcagg gacccggtat tcatacattc gatcgccatt ttagacaccg atggaacaaa
    25381 tcttattgac acctttgaag actacaaggg tcaaacagag aatagcttga tatatttcgt
    25441 tcgtccaatg caaacaggcc ttccatttgc gtccaacatt ctattccaga acaatcgaaa
    25501 ccacatatat ttttccgcac ccatcaaatc acaaaacgga aacaccattg gcatcctgcg
    25561 agttgaatat cacgccacaa taattcaggc aattgtgcgc tcagtcgacc ctcttaatgc
    25621 aggaacaacc ctgtcgcttg tagacactaa tacatatctg cgtgtggcct acacaggtga
    25681 ccgtgacgaa cttttcaaga cctataaaga tttttcggcg cctgatctcg cagttttgca
    25741 atctgaagga cggctgcctt ccatctcaat agaaagtctt ttaacggaaa cagatgttgc
    25801 aatggtggat ggcattgaca atctacaaca acaatcattt ttcgatgcca catcaaaatc
    25861 actcgggtca accacaaaca ctggtgtttt tctggaaacc caaccgtggg ttgcaatcgt
    25921 aaggcaatcc aaatccaatt atctggcacc agtagaagac caaaacagga cgaccatcct
    25981 gatttcactg atcatggtca tcctgagcat tggcgcgggg ttccttgcct cccaagtgat
    26041 cacctcaccg ctcctgactc ttgcaaaaac cgccgaacaa atcgcagtcg gcaatcgttc
    26101 ggcacgcgct gaggcagtga cagaagatga aatcggcttg cttgccaagt cattcaaccg
    26161 catgacagat gagctaaacc aagccttcaa aaaccttgaa attcgtgttg tagaaagaac
    26221 caccgacctt gaaatcgccc gtcgtcaaag cgaaaaacgc gccagcgaac tacaagccat
    26281 tggcgaaatc tcaaaagtta tcacaggcga acaagccctc gaaaaacttc ttccattgat
    26341 ctccaggctc gtaagtgaac gtttcggctt ttatcacaca ggtatcttcc tgctaaatga
    26401 gacaaaccag tttgctgttt tgcaggcagc cagcagtgag ggcggccaaa aaatgcttga
    26461 acgcggacac aaactcgaag tcggcgaaag tggaatcgtt ggttatgttg caaaattcgg
    26521 aatgccccgc atctcgcttg acgttgggca tgatgcggtt ttcttcaaca atcccgacct
    26581 ccccaacacg cgctctgaaa tggcactacc tcttaaggtc cgcaatcgta ttgtaggcgt
    26641 acttgatgtg caaagtgaaa aaccaggcgc gtttactgaa tacgatgcca gtacacttag
    26701 tattcttgcc gaccaaatcg caattgccct cgaaaacgcc cgcttgttca cacaaaatca
    26761 acaagctctt gctgaggctc aggcgcagta tcgtcaaaac ctgcaaagca gttggagagt
    26821 gtttagtcgg gaagaagaga ccattgggta ccaccagggc ttaatgggtg gcaaaaaaat
    26881 atcaaaaccc gttgagaccg aagaaatccg cgaagcaatc aaccgcggaa acgtcctcgt
    26941 gcttaattca gaaacagaca agcgagaagc aacgatcgtt gtcccgatca aactacgtgg
    27001 acagattatt ggcacaatga acatcaaagc accttcagca aaccgtacct ggtccagaaa
    27061 tgaagtcaat cttgccgagg caatttcaga gcgtctttcc ctagcccttg aaaatgcccg
    27121 cctgattcag gaatcgcaaa gacaggtaat caaggaacaa accatcagtg aagttactgg
    27181 aaagatcggc gcatccatca acttaaagag tgtacttcaa acggcagtag aagagttagg
    27241 ccacgctatt ccggggtccg aagtactcat tgaattcgaa caaaagaacg agaatatgga
    27301 gtaatcccct aatgactgct gcttattcag aaacccccat attccaaaac agggaacaag
    27361 agcaacgtga gcgtattgcg ctgacagttg caagagcatt ccttgtcacc agcacgatca
    27421 tttcattctc cattctttac attgccatca gccttaacag ttggcagctt tttactttgt
    27481 atggtggcaa tattttatat atccttttga catatgctgg cttgcaacag cttcaaaaga
    27541 aaaacacctt gtttgggatt tttctagtaa ctggttcagt ccacttaaca atcatactat
    27601 tgccgattct attcgaaggc atagccaccc tggctggtct ttttagcacg ttggcagcag
    27661 cactaatttt gagtcagacc cttgagacca gggttgcccg aggggctatc gtagtcagca
    27721 atatcactgg gatcggcatc atcctgcttg gatccatccc gtttcccgtt agaattcaaa
    27781 tcccaagcgt tttggtggtt acaatagcca tccttgcgat aattgaaact gccattttta
    27841 tggtattgat ctggcgtcaa tttagaacat ttaacatcca aacaaaactg acgcttgtct
    27901 ttattacaat tcttgtcaca tcattgctgg tcacaggtgg ttacaatgcc gtccaaagct
    27961 acagaactca gcgggcacaa accgcaaaca acctggaaaa cgaacttggt gtaaaacgat
    28021 cggtcattat cgatttcctc gaagcttcca gacaggatgt agtcttcctc ggagaagcgg
    28081 aagttgtaca aagttatgta aagacgatcg atgacattgc tgctcccaac attgttgtca
    28141 gagcgagaac catcatggag cgtgaattcc gccgttttgc agaaagccgc gggatttatc
    28201 aagaaatacg cttcctgaac aacgacggga acgaagtaat tcgaatagat accgctctgg
    28261 atggtaattc atccattgtg ccgcaggcgg aactacggca gaaaggtgaa cttgatcctg
    28321 caagggaatt aagctatttc gcgcagacaa tcaccctgcc agctggtcgt ttattccagt
    28381 ctccaatcaa cctcagaaaa ctggatggaa cgatcgtcac accacatgag ccgatcattc
    28441 aatttggtgt acctctcgtc attaacacgc aaaccaaagg cgtgatcatg gcaaatatct
    28501 atgccgagaa attcctcggg attctctccg catcaggtga tgatactttt ttggtggaca
    28561 caaacggtta ctacattgcc catcctgatg aatccaagag gtggggcaac gatctaaaca
    28621 acaatataac catcaaaaat gactttccag acctcgtaaa caatcttaac aatggaaact
    28681 ctggaagtat ggagtcaaat ggatatcttg taatctatac ccccatcacg atccctggcg
    28741 aaacagaacc acgctggtat attggctcat ttgtctccat agactcgatc acaaaaccga
    28801 tcttcgaatc aacctacaat gcgttatctt tgttattgct aacgatcatc gcctctattt
    28861 ttattatgag caccctgagc aattcaatta cttctccact gggcaatctg gcactggctg
    28921 cacaagatgt tgctgcaggg aaattatctg ctcgcgttaa tcttcaaacc aatgacgaaa
    28981 tcggtgcatt ggcatccatt ttcaactcaa tgaccgacca actccgagag acggtatcca
    29041 gtctcgaagc ccgagtcgca gaccgtaccg cagaacttgc aaaagaaaaa caacagtctg
    29101 acacacgcgc aaaacagttt gaggccatta ccaaggtggc ccgcgcaatc agcgccaaaa
    29161 gcaacttgca ggatttgctt cctcaaattt ccagggtgat cagtgagcaa ttcggttttt
    29221 atcacgtggg aattttcctc aatgaccccg ccaatcatat ggctgttcta agcgcagcaa
    29281 atagtgatgg tggaaaaaag atgctgcaac gcagtcatca attaagaatc ggtgaacaag
    29341 gtattgtggg ttatgtggca caaacaggga acccgcgtat tgcccttgat gtcggtgaag
    29401 atgcgaattt cttcaacaac ccagacctgc cagacacgca ctctgaaatg gcactgccgc
    29461 tcaaggccag taacaaaatc attggcgtgc ttgatattca aagcacaatg accgccgcgt
    29521 ttaccgatga ggatttcgag gccctttcag cacttgccga ccaagtgagc ctggcaattg
    29581 agaatgcccg cctctttgat gagaccgaaa aaatgctcgc cgaatccaga gcgatacaac
    29641 gccaaaatac tcttgagact tggcaacgct tgccccaaga agagaatttg cgcggttttc
    29701 gttattcgat tatcggcgcc agccctctca agacaagtga gacaaattcg acacaggttg
    29761 atacgagtga aaagagaatc attaaagtac cgatcaatct ccgtggcgaa agaattggga
    29821 cactttcggt acaggtcgca aaacacgaac atgtcagttc cgaccagtta gatttgatca
    29881 aagctgtagc agaacgagta gctttatctg ccgaaaacgc acgcttgttt gaggaaacga
    29941 cccgccgcgc agaacaggaa cgtatcattg cagaaataac atcaagaatc ggcacatctg
    30001 taaggactga aaacattcta cgcacaaccg catccgaact aagtcaacta ctggaagatg
    30061 cggatattta tattgaactg caaagcaagc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30181 nnnnnnnnnn gaactaagtc aactactgga agatgcggat atttatattg aactgcaaag
    30241 caagccacag gacacaaagt agaaatcttt agaggccgta tgcaagagaa aagttcctac
    30301 catcaaacta gcccgaacaa acaacagttg gatcgaaacg cttttaatat cagcgttatt
    30361 actgccctgc ttcttggtct ggcactaggt gtcgcctatt tactgtcccc cacttcattg
    30421 gacacaatta gtttggtggg gttgttggca ggcattttct ctgctggtac tagcatctgg
    30481 ttgagtcgtc gccaaaagag cgagatcggc ataggaatcc ttataactgc actgatcgca
    30541 atctctgttt cccaggtttt tgttagaaag ggacaggcat tagctacaag cattacaaat
    30601 attattatca tttcaggaat ctctttttac actctacgac ggaaatgggc tggctgggct
    30661 attatggcct ctttcttgat ggcatctgtt acggtcgtta tagatcaatt cacaacggga
    30721 gttccagagt catctcggcc agaggctgca acagccattg ctgtgggctt aagccttata
    30781 tatctggtaa tcatcgcact acagttcaag aatttcccgt tacgcgtgaa attgatcttt
    30841 ggtttcttgg ttttgaccac cctgcctctt ttgattctcg gctggcaaac caacctggct
    30901 acccgtgaaa tcttgcgaga agagataaaa tccagcattc tcgaaagcac attaagcata
    30961 agtgacgtgt tccaagagtt cgtagattca cagcatgttg ccattggtaa acaggcacgt
    31021 actcctgaat tgacagattt tcttagcctg cctgctccag aaagagcgga ttccgatctg
    31081 gaggctgcag cactggaaac attgacaaag ttcaaagatg aaagcgcttc acatattatc
    31141 gcctattcgt tggtggacct cggcggcaga aatattttaa gcacagatcc atcggcgatc
    31201 ggcgtcagtt acacagatca agccttcatt gcctcggcaa ttaccgatca caaacctcat
    31261 gcttcgggcc tctatttaac ccatcaacct gatgtccgaa tcatctactt cgctactccg
    31321 atagtgtcca aaacgaacga ttcactcggt gtccttctgg tcacctacaa cgcaaaaatt
    31381 cttcaagctg tcatggatac aatcttgaga gggcggccag taatcaatac agaccaatat
    31441 tcattcttaa ttgacaaaac aggctttttc gttctggcac attcgtcctt tctcacactg
    31501 gatttcaaaa catatctcgg tgaaaatgac ccacggctga aagttcttca agaacaggct
    31561 ggaaccgacc tggatgcgat caaagccttc aacattcctc aacctgagat tgtggaagct
    31621 attaaatcag caccggaagg aacttcctct ttccaaattc cttccgttga aaatgatggc
    31681 aaacttgcag aatctgccgc aaacacgatt tcaaattctg attgggtgtt ggtcacatcg
    31741 caacccgtca gcacgatttc aaaagccaca agcggacaaa cccgcaacac cgtcctcatc
    31801 agtattgcaa tcgtttttat tgcagcagta agtgcagtgg cagtctccaa tgtgttcaca
    31861 aatccattgc aacaattgac aagtgtggca gaaaaaatct ctgcaggcga cttttcacag
    31921 caggctgaaa taaaaactca tgacgaaatc ggccttcttg caggtgccat caacgaaatg
    31981 gcatcgcaga tccgaggctt tatcgccagt ctcgaaaacc gtgttgaaca gcgcacactt
    32041 gaattggcac aaaggacaaa tgaacttgaa aagctcacag aacaaagcaa aaaacgcgct
    32101 gatgagcttc aaatcattgc tgaaatttca ggctacattt cagcagaaaa agacctcgaa
    32161 agcctgctcc cattaattac aaaaactgtg agcgaacgct ttggctttta tcatgtcggt
    32221 atctttctgc taaatgaaaa tgggaaattt gccgttctac gtgcagccaa cagcccaggc
    32281 ggacaggtaa tgttgaaacg tcaacataaa cttgaagtcg gccaagtcgg tattgtaggc
    32341 aatgtgacag ccaccggcat tgcccgtatc gctcttgata caggcgcaga ttcgacattt
    32401 ttcaataatc cagacctgcc tgaaacgcat tccgaaatgg cattaccttt gattgcccga
    32461 ggtacaatca ttggagcact tgacgtacaa agcactttcc ccaacgcatt tacccagaac
    32521 gacatctcca ttctgggatt attagccgac caaatcgcca ttgccattga caatgtccgc
    32581 ctgttggacg aaaccaaaaa ggcactcgca gaagcacaat cctctttccg tgaatacctt
    32641 gccggggcat ggcaaaacaa atctgcctct gagattttcg ggtattatca aaccctgacc
    32701 ggcggccaac tcattactgg agaaaagaca ccggaagtag tcgccaaaac cgaaaaagac
    32761 gagaacgcgc tgtccatccc cattcaactg cgcgatcaag tgattggcac cgttcacatc
    32821 cgcccaaata aggaagatag agcctggagc agggatgaga ttaacattgt caatgccgtg
    32881 gcagaacgcc ttggcctcgc cctggataat gcccgcctct tcgaagaaac atcgtcacgt
    32941 gcttcgcgcg aacggttggt aacagaaatc accaccaaga ttcgcggcac aaacgatccg
    33001 caagaaatga ttagaactgc tgtacaggaa cttcaacgcg cattgggagc atcccgagtc
    33061 gagatcgttc ccagaaaaaa cgcccctcct cctgataaat agactggatg gtaaacaatg
    33121 atcaaaaaaa tcgctatatc caatgaaaaa ggcggtgtag caaaaaccac ctccacaata
    33181 tcactcggcg ccgccctggt ggacatggga aaccgtgttc tgctggtaga ccttgatgcc
    33241 caagccaacc tcactctcgc cctgggtctt gagccaggcg aatctgcaat cacatccagc
    33301 gaagtaatac tcgataacat acctctcctg aatgcgctgc gaaaaactga aatcgagaac
    33361 ctcgacctca tcccatccaa ttctcgcatc gaaaccgccg aacaattttt acccgtacgc
    33421 accagttaca ccacaacatt acaacgcgca attgccagcg cacctaattt gcattacgac
    33481 tatatcctgt tcgattgtcc acctgcattg ggcgccatca cacaaaatgc gcttatcgcc
    33541 gccgaccttc tcattatccc tacacaagcc gaatattttt cggcatacgc acttcggaac
    33601 atgatgacct tgatacgaag aattcgcgag ggcgaaaacc ccaacctggc ctaccgcgta
    33661 cttgtcacca tgctcgacaa acgtaatcgc acacatcgca acatccacga ccagctacgt
    33721 tctacttttg gtgaaggtgt gttcagcacc gtgatcgaaa ctgacacaaa attacgagaa
    33781 agccccatcg ccggtttgcc catttcccac taccgccccg gcgcacgtgg ttcaatccaa
    33841 taccgtgtgc tcgcgcagga gttgatcgaa tatgccaaag aaaccgaaaa tagacaaacg
    33901 gcttaacaag ctattcaaag acataaatct ggaagaagct tcctccagct tgaaacagga
    33961 agcaaaagac gaggctgaat ctaaagcctc ggtgacagaa acagctcccg cgtcaaaacc
    34021 agacccgaag cctgcttcca aaaaaacgga tacccgcccc atcccgaggc agggtcgtat
    34081 tcacacatct acactgattc ctcccgagcc tgtcattacc gaagaacatg agaatattgc
    34141 atccacctat gcggtaaaca tccaaactgg ttaccaagac tgggccacac tgcgcatttt
    34201 ggatgaaaca acacaacgcc agtggtcaca agatgaagaa ttgcttgtta aacaagttac
    34261 cgatcaatta tcccttgcgc tggaaaacgc ccaacttttt caggaaaccc aaaaaagagc
    34321 caacgaactc gccacactaa acgaaattgt gcaggcgatt tccgaacaga tcgaactcaa
    34381 aaacgtctta aacaccgcct acgaacaaat cgtcaaactg atcccgcttg atgcattttt
    34441 tgtcgctctc tacgacgaat catcaaacac agtttcctac ccaatagtta ttgatgaagg
    34501 caaatcgtat gaggaaccca acgaggcact caaccctgaa aacttcacag ggagagtcat
    34561 caagtctgga aagttgcttc tggaagccgt aaagcctgaa gaccttaaaa gcaaatcaat
    34621 caacagcgac tctttcggca ataaaaacaa gaattatgga accatgttgt tcctgcccct
    34681 gaggtctgtt aacaagacca tggggtgtct ttcaattcaa aactacgact atttcgctta
    34741 caagcaggaa gatatcgctc tgcttgaaaa catcgccaat cagatatcga tcggtattca
    34801 gaacgcccag ctcttcaacg acgcaaaaca gagaaatgaa gaacttgccg tactcaacgg
    34861 attgagcggc gaactctcaa cattggttgg tatcaacaat gtagccgaat cagttttcaa
    34921 atacacaagc aagttgatga gaacaagttc cttcttcctg tgcctgttgg acgaaaagca
    34981 aatggaagta agttttccgc ttgtgattga cgatggagaa aaagtagaag tagcaccgcg
    35041 aaaggcaggc ggaaatggtt tgacagatta tgtcatgcga acaaaccaaa ccttgtttct
    35101 gcccaatcgg gtgaacgagc gctctcttgc gctcggaatt aacatcacga tcattggaaa
    35161 ccccacatca gccaagagtt ggctgggagt gccccttgcc ctcggtaacc gcatccttgg
    35221 ggcgatcgtt gtgcaagacc ctgaggaaga gaacgtctac actacacacc agcgtgatgt
    35281 tctcatcacc attgcccaac aagccgcagt agctatcgaa aacacacgcc tctttcaaga
    35341 aacagaaaag cgcgccgacg aactttctct aatcaaccgt gtggttagca cactaactgg
    35401 cgccaccaac ctgaataaca cgctgggtct cgtgattcgt gaactggtgg aagcattccc
    35461 agtttcgcgt ggggccattg ccctgctcaa cgatgaaaaa caaagtttca tcgtagttgc
    35521 ggagcattca aaacaagata atcataattc ggcattgggg cttgaaattc ccatcaaagg
    35581 aaatacatca accgaatcga ttatttcaac tcgaaaacct tacattcttg aagatgctgc
    35641 caataaccct gccaacccgc cagaaatacg tgaagagttc cagcgattag gtattgttta
    35701 ttcggctatc tttccaatca tcgtcaacga tactgtaatc ggctcccttg gcgtggatac
    35761 aactcaaaaa gatacccgtc tggacccgaa cgagatcaca ttaatcgaaa caaccctttc
    35821 gccaatatca gcaacaatca gccgtctgaa tgcagaagaa gcgctctcac gttcagaagc
    35881 cgacctgcgt acccttttct cctcaatgga agacgtggtg cttgtgttcg acgcagatgg
    35941 ccgttatata cgcattgcgc caacaaaccc aggtcgtttg gtacgcccgc cagaagatct
    36001 gctcggaaaa ctcatgcacg aggtattgcc tgcaaacgtt gcagatcaat cagtagctgc
    36061 cattcggcat actcttgaaa caggtgagac caatcaactg gaatatgagc tccagatcgg
    36121 tgaagagaaa ttctggttcc ttgcaaacct gaccaaactc aaagatgatc aggtgttctg
    36181 ggttgcacgt gatattaccg accgcaaaaa ggccgaggaa tccattcgcc gccgcaatgc
    36241 gtatctggcg gcctcttcgg aaattggcaa gatcgttaca tccacacttg accttaacac
    36301 aatttttgcc cgtaccgtta acttgatcaa cgaacgcttt ggctttttcc atgcaagtat
    36361 ttttattgtg gaagagacag gattcaacgc cttattacgc gaatcaacag gcgccgccgg
    36421 tgcggaaatg aaaaagaaca agcactctct ccccgtcaat gataaatcca tcgtcggcaa
    36481 ggtggcattg gatgggcagg ctatcgttgt gaatgatgtc aaaattgact cgcttcacaa
    36541 attcaacccc ctcctgcctg aaacagctgc cgaagccgcc attccactcc gaattggcaa
    36601 tcgcgtcatc ggcgtcattg acgttcaatc caagtctaca aactctttta cggatgacga
    36661 aatatcggtg cttcaaacag tggcagacca ggtggcggta gccattgaca atgcccgatc
    36721 ctttgaactc tcgcaacagg cggtcatgga aatgcgtgaa atcgaccgcc tcaagagtca
    36781 attcctcgcc aatatgagcc atgaattacg cacaccgctt aactccatta tcggtttttc
    36841 gcgggtgatc atcaaaggaa ttgacggccc ggtaacagaa ctacagcaac aagatctcac
    36901 cgccatctat aattcaggtc agcatctgct tggtcttatc aatgacattc ttgacctttc
    36961 gaagatcgaa gctggcaaga tggaactggc cttcgatgaa gtcaacattg tggatatcac
    37021 atccagtgta ctctccacca tgagcggtct catcaaagat aaacccatcc agatgaagcg
    37081 tattctcgaa cccaacctgc cgacggttcg agcagatgcc attcgtattc ggcaggttat
    37141 gatcaacctg ttatccaatg cggctaaatt caccgatgaa ggtgatattc ttgttgaagt
    37201 gggactcaaa cctgggcagg gatccacccg ccgcgaatta agagtcagcg taaccgatac
    37261 aggccctggt atttccgttc aggatcaggc aaaattattc cagccattct ctcaggtgga
    37321 cgattcacca actcgtaaaa ctggcggtac aggtttgggc ctgtccatct gccaacatat
    37381 tgtcaacatg cacggcggac gaatttggat cgaaagtgaa gttgggaaag gcagcacttt
    37441 ccatttcaca cttccgcttt accgaaagga agaagaggat gcagacagtg gaaggaagac
    37501 tgtcctgacc attgatgatg accctcaagt gatcggactg tatgaaagat atttacagca
    37561 acaagggtat caagttgtac cgctttccga cccatccatg gctgtggaac gtgcaaaaca
    37621 ggtgaaacca tttgccatca cactcgatgt gatgatgcca gggatcgatg gctggcaggt
    37681 tttggataaa ttaaaagccg acccggaaac gcgcaacatt ccggtcattg tctgtagtat
    37741 cattgaggaa ttggagaagg gcttcaacct cggcgcgacc gattatctgg tcaaacccat
    37801 ccttgaagat gaccttgtca atgcgcttga ccgtcttaac tctgacggct caattcgtga
    37861 agtccttgta attgacgacg acccggacga cctgcgcctg atcggaaaaa tcctaactga
    37921 tgatggccgt tacaaagcct cactcgctga aggcggccgc atgggatggg atatcatttc
    37981 ctcgggcaat cctccacatg ctgtgattct cgatctgttc atggaagaga tggatggatt
    38041 tagaattctg gaaaacatgc aggcaagtga aaagctccgt aacattccag tcatcattgt
    38101 cagtggaatg gatctttccg ccgagcaaaa agaaaaatta aatgaatccg gtcagcgctt
    38161 gctgacaaag ggcaccttca gtgaaaaaga attattgatt tcgatccaac gttcattgga
    38221 gaaagcccag gcgaaaaata aataggagtt ttcaatatcg tgtcatttat cataaagtgc
    38281 cttgattgtg gacataacgc accgtattat cccacgtcta cgaactgccc gaagtgcaac
    38341 agccagtggc gtgaagcaga atacgattat gagcttattg ggaaaaccct gccagccaag
    38401 ctggcagggc gtccttttga cctctggcgc tatcgcgaat tattgcctgt acgtaatccc
    38461 aatgtgagtc tttctcttgg agaaggcggc tcaccattga tacgcgccgt aaatctgggg
    38521 atgatgcttg gttgccccaa tatttacatt aaggatgagc gtcaaggtcc aacatcttct
    38581 ttcaaggata ggcaggcttc agttacgatt gctgcgctta aagaggcggg aattacagaa
    38641 atggtggctg catccaccgg gaatgtggca atttcctatt cggcatacgc atcccgagca
    38701 gggatgaaat tatgggcatt tgtcaccagt cttgtaccag ccacaaaaat gcgtgagatt
    38761 gcgttgtatg gaagtcaggt tatcaagatc acgggctcat acgatcaatg caaacaggtc
    38821 gcttctgaat ttgcccgtca gcgccatttg tatcaggaca tgggcgcgcg cactattacc
    38881 tccattgaag cgatgaaaac cattgctttc gaaattgcag aacaattaac agcgatccag
    38941 gggacggatg gggccgcccc atggcgcaca cctgattggt atgtgcaggc cattagcggc
    39001 ggcatggggc caattggtgt gtataagggt ttccgcgaaa tgaaagatat gggatgggtc
    39061 aatcgaattc ctgcatttgc ccccattcaa gcggagggct gtgcccccat ggtggatagc
    39121 tggaaaaagg gcttggaaaa agccgaaccc gtcgtatcgc ctaaaacccg cattgaaact
    39181 ttggcgaccg gtgacccagg ccgtgcctac gaatttttac gttcctacgt caacggcacc
    39241 aacggtgcgt tcgaaagtgt cagtgatgaa gatgcgtatc gtgccatgca cgtattggca
    39301 aaaatggaag gcatttcagc tgagcctgct gcagctgttg cgtttgcagg tctattcaaa
    39361 ttgatccgtg cgggcatcat caaaccgagt gacacagtgg tggtcaactg cacagggcac
    39421 accatgcccg ctgaaccgat cattctcggt gaaaactggt caagagatgt caaattacca
    39481 accagtcagg aaacaccgca ggaaggtcta ctggcagcac ttacacaggt ggcgcctgag
    39541 cgtttcccca agattgccat tgttgaagat actactgaag cacgccgcct tattcggcgc
    39601 attttgcaat cgcagggtaa tttcaccatt ctggaagcag gcaatggtcg cgaaggactc
    39661 gaattgatcc aacgagaact gcctgacctg atcattcttg atctgatgat gcctgaaatg
    39721 gacgggttca ctgtaatcga agccttacgt gccaaacagg aaaccgccac catcccggtg
    39781 attgtggcta ctgccaaaga acttaccccc gatgaaaaga acagacttag tggacaaatt
    39841 caatccctta tgcaaaaagg cgatttcctg aacgacgaat tcctggaaga agtaaaaacg
    39901 ctcatcaaat agctttcaaa atgaccatag gctggtacct ttgcagaaaa aacatacggc
    39961 cggagttctt gccgccttaa gttcagctgt atttctcggg ttagcacctg ttctgggaaa
    40021 actggcgatc acattgggtt tctcgccgtt tgcagtggtg gcacttcgga ctgggtttgc
    40081 cgctggtatt cttttaatat ccatcaccct attctctcct caatatttat acatcttccc
    40141 tgtggggttg gttggatgta tgtttgctgg agccatcaac ggtcttggct ccctgcttta
    40201 ttatcttgcc ctgcaacggc tgcacgccaa tgttgggcaa ttgctttact cgctttatcc
    40261 atttttcgtt gccctctggc tgatccttga ccaccaggca ccaagccgat taacacttta
    40321 tcgcatcagc cttgctacgg cagccgtggc attgctcacc ggcgggccac aacaacaaat
    40381 agacatcgtg ggcgttttta tgatgatcgg cgctgccatc ttgtatgcca tgcatttgcc
    40441 gatcaatcag cgcgtgcttt atgaagtacc agcacccact gtggcgttat acactcttct
    40501 ttcgatgagc gctgtggttg ttcccgttta cttgatcttt gaccgggcat ggcccattgg
    40561 ggaagtaccc tggttaccag tcatcgggct gacatttgca actgtttttt cgcgtattgc
    40621 cttattttta ggggtgaaga aaatcggtgg aatgcaaact gccctgctgg gacttgccga
    40681 attattgata gccatcatct tcagcgacat atggcttggc gaaaacctat ctctccttca
    40741 gtggcttggc gcagctggac ttggcctgag cctgatcctc gttatgtatg aagatacaaa
    40801 ccctccaaca tatagcaaaa gaacaggctg gctctcgtgg attcgtgcgc cagacctgcc
    40861 aaaagatatt tttggtccct acgaatgaaa atcaacaaac aggtgaccga gtagaatata
    40921 gaagacgctg attttgccct tgttagagcc aatcagcgtc ttcatctcgt ttgtacgaat
    40981 gcaagtgtca aaatccaaaa ttggaatttc gggggtaatt actttttacg cctatttatt
    41041 accttcgtac aagtatcccg tccctcgcac actcaccacc cgttcagaga gtgagggaaa
    41101 ttgatccagc ttatggcgca ggcggctaac cagcggacgt aaaatctccg gagcctcgcg
    41161 ctgagaggtg tcgtaacctt gtaccagtaa aaccagttca cgatgtgaaa acactttgcc
    41221 ttcgttttca atcaagattc gcaacaacct gccttcagcg ggtgtgaggt gttcgatctt
    41281 gttcttgtgt ttgatctgtc tgcgggaaag atctacagtg gtaccatctt taagggaaaa
    41341 gactgacatt gcctcgtcta cgtcagcagg agtcacattg ctggtacgtg actttgcagt
    41401 tcgacgggca agacctttct ttacacttgc caatacctgt gcaggagagg caggcttcaa
    41461 caaataatcg tggatgcgca atcgcagggc ttgaatggcc gtttcagtgg agccaaaggc
    41521 tgtgagcaaa atcacctcgg tttcaggtga aatctgattc accacctgca ccacttccaa
    41581 accatccatg ccaggcatgc gcaggtctac gatcatcagg tcgatgggat gtgtgcgcac
    41641 atactccacc gcggcctgcc cgttcggagc ggctgtaacg ttgtggcctt cgagcttcaa
    41701 aatatctgcc agagactgac gggcaacggg ctcatcatcg accactagaa tgttggactt
    41761 cattgttctc ctcctatggg taagaatatt cgaaaacatg caccggggcc gcgttctggg
    41821 agcaattcca gtacgccgcc atgcgcggtg attatattgt aactcacagt taaacccaac
    41881 cctgtcccgc catcttttgt gctgaagaac ggctcaaaaa tattggattg cttttcttca
    41941 gggatacctt tgccctggtc ctggaacaat aattcgacac ctcctttgga ttgacgagca
    42001 atgatcttga gcaccccgcc ttcgggcatg gcatctacgg cattcaagat caaattaata
    42061 aacacctgct ggatctggct gccgaccgct tgtatgggtg aaatcttccc aaagaaatca
    42121 acaacaacct tcacattcgc ttcagacagt tgttttgaca tcaaattcaa aatatattga
    42181 agcatttcaa ccaggtcaac tgtttcaagc gaggaggcgc ccggacggta aaaatcaagc
    42241 atgcgccgca cggtgaccgt taaacgctca agctcggtgc gggcaaggtt gaagtattcc
    42301 tggcgttttt cggcgggcag gtcttctcga cctgcaaggt gcagacagtt ctgcacagcc
    42361 tgcagcgggg tattgacctc atgcgcaatg gatgcactca agcgacccgc tgccgccatc
    42421 ttctctgcct gcagcagggc cttttgtgaa tcttccactt tgcggacgta atccaattgc
    42481 tcggcataca gacgagaatt ttccagtgcg acagccgcct gacgggcaag aatttggaac
    42541 aactccagat ctgattcgcg aaagggcggt tcgttcaggt tgcgtgcggc atacaaaacc
    42601 atgcgcaggt ttgggcgctg aatcggcgtc aggatcgctg caccaagcct caggtcggtg
    42661 agaagcgatc tcagggtggt gttcccaggc ccactggcat tgagaagcaa aggcgtggca
    42721 gattcatcga tgtgggaaat caagttaaga tggttcttcg aaatttcacg gccgcgggaa
    42781 gccacgagtg taaactcatg cgtttcgttg ttttgctggt agcaggcagc atgagaacat
    42841 tgcaggtgcc cgcaaatggc gctgaggatg agatcaagca gagggtcacg gcgcgtttct
    42901 gaaagcaggg attctgtaac cgagaagagc gggcgcaatg cctgtgtgcg ggcggcatcg
    42961 cgcttttgct gcgaatcggc aagggctagt ttgaccgcgt caacaaggtc gctgcctttg
    43021 ctgaacggtt taagcaaaag cccatccact ccctgacgca aagcacgaat ggctgtctcc
    43081 actgtaccat gcccggtcat gatgagcatg gccgcatcgg gttgaagccg ctgcgcatgt
    43141 tgaatgactt caaagccatc cacttcaggc atgcggatgt caaccaacaa caggtcgatc
    43201 ttattttcct tgaggtagtt tatggctttg cgcgggtcgg tctctgattc caccgtgtac
    43261 cccgaacgcg agagcaggcg tttgcaaagc attgcaatgc caggttcatc atcaacgaca
    43321 aagacgaagg gagtttccat gtttagatcg gcagccaaac tgtgaatgta gagccttttc
    43381 caggctcgct tgcaatgtca atacttccac cgtgatctgt gacaatgccg taagttacag
    43441 aaagccccag gcccgtgccg ccgctatctc cttttgtggt gaagaatggc tcgaagatgc
    43501 gatcttgatt tgcgggaaga atgccaatcc cgctgtcttt gaccgccatg ataacccaat
    43561 cgcgttctgc acgcacggct gaatcagttg atacaaacaa atcgccgcct ttgggcatgg
    43621 cttgtaaagc gttgtggatc aggttgagta aaacctgttt catttgattg cggtcaatgg
    43681 aaacccaggg caggctgctg ctcaaaccca atgtgagatt taccccgttg gtttgaatga
    43741 gatgcctggt gagtgctatg acatcattga ccacttcgtt caaatcggca cgggtgcgga
    43801 tgcgttctcc ctgccgcgaa aaatccagca gacgatggac aacgtcgctg gctcggcgtg
    43861 cttcacgcag aaccatttcc aaatcatcac gatgcgacga accttcaggc agttcctcca
    43921 aaacaagttc ggagaagcct gtcaccgtgg taagcggatt gttcaactcg tgggcaatgc
    43981 ctgcggccat ttcacccact gcggcaagtt tcgctgcctg aatgaggcgg ttttcagcgg
    44041 aacgctgtgc ttcaattcgg gaattcaact cgatctcgat cgcccttaac tgacggatgg
    44101 tctcttgcag cttttgatat tggtttgcgc tggaaatgac ggaagccaaa atacccgcca
    44161 gtgactccat ggcgatcagg tcattgtgcg agaaggcatt ctgttcacgg ctttcaacat
    44221 caatgatgcc gagaatattt tcaccatctt tcaaggccac acaaatttca gagtgtgcct
    44281 cccagccttt gagaggcttg tagagctttt cctgccttgt gtcgttgatc aaaatacttt
    44341 cgccgctctg agagacaagc cccgtcaccc cttcacggac gatatattcg ccgttgccct
    44401 gatctgcaag cgctttctga accactgccg cctgtgtgcc accgatgcct tgaatcgaaa
    44461 aacttccctc ttcatccacc agcagaatgg cggccaattc atatttgaaa tattgcgcaa
    44521 ccaattcagc ggcaatgtgc gccacttctt ttttatcgtt caagccaatg acctgctgta
    44581 ccacttcgtg gatcagcccc aggctgcggg cacggccttc ggcctcttcg cgcaggcggg
    44641 tgtattcgat caaccctgcc atgtggctgg tgataaccac catcaaatgt tcatcgtact
    44701 gactgtaggc ttctgcatgc aggttttcaa taatgaggac gccgatggct tgtccgcggt
    44761 atttgagggg gacgatcaac gccgagcgtg cgcctttgaa aacggggaca aatccctctt
    44821 tgatggcgtc tgtgacacgg cgcaaacgtg cttccttgag tatgggttga atggaatgcc
    44881 cagccaaaga aacgctgatg acatttaatt tgccgtcaac catgcggtag tcgcgcaaca
    44941 cacggccgtc actggagcgc aggaagaggg cgatcaactc ggtgttgaag gcacgagcca
    45001 gcagaccaaa catgcggcgg gcaacctgat cgaggctttg agcggaggaa accgtgagga
    45061 caaaatcatt gagcaagccc agcctgcgca gatgggctgc catttctgaa aaggtgacca
    45121 gcacttccac ggacggagag acgcgctggg caagatcgcg caattgcccc aactcactgc
    45181 ttgaaaattc ggtctgacgc cagagcgcca ccaccccgat caggcgttga ccaatgacca
    45241 ggggcaggca gatccatatc cgtgtgtttg gttttagcgg gccgcatgga atcgcatccc
    45301 aatttgggtg tgtacgcgaa accaccaagt cagaaagaga acgattgacg cggcgcagaa
    45361 tgggatttgt atcaatggac agggaaaggt ttttggcttt cgaatcgttc cactcggctt
    45421 gaatatcgag cgattcgccg cgtcggatcg ccagccacgc gccctgacaa ttcacggtct
    45481 gcacgatggt gttcaacact ttttcgatgg caagcggcag atcggaggcc aaaccagatt
    45541 gcaggtcagg tagaaacggt tgatctggca gggaggaatg attcgagagg agcgaggcgg
    45601 tcagtttcca gatccgctgg gcggtgggag attgtttatc cgccccaacc aaaacagccc
    45661 gtgatgaccc cgtaacagga aaaacgaaga agcgactccc agcgagtccg ctcccttcag
    45721 gaatggagct tgaacgggga tgccccccac tcaaggcgcc gcacatccag gcatcaatgg
    45781 acggccttgc catgatggct gccaactcgg tttgggccga acggtttaag tggaaaactc
    45841 cgcgcaagag ccatgtgccg ccaacgcgct ctgcaagaac agcccactcg gcttcggaaa
    45901 gttgcgcggc atctttgagt tgagactcga ggctgatttc agtctgcatg gcggtatgcc
    45961 aaattataca ctcccccctg cgtctattgc caaaattggc tgttcaagaa caaggctctg
    46021 cccgcccaaa ttccaacact cggaatctgg gcgggatgca ttctaattgt tatttttctc
    46081 ctgcttgtct ttttgcttct gccaaaactc ccgccactta gccacccatt gctcttcgga
    46141 catgtcatcc gtttttttgc ccgtcagatt aaagtatatg cccaggatcc aatctttgaa
    46201 tgcttgcaat gattcgtctt tcggagcgga ataatcgcgg ccttccgcct tattcatctg
    46261 ccgacctttc aggcttggaa agtttttcca actgtgcgcg gatctgacca agcaaaagct
    46321 cgatctcttt gcgttcttcg atggcgtggc gcagggaggt ttcgatggat accttctccg
    46381 ccccgctcaa ggtccgcacc aggtgttcgt cgcgggtgat ctgggtgagt ttgtggttta
    46441 gttttgtctc aaggcgtttg cgatagccat cgtaaaactc aaattcattg gggagttttg
    46501 tcgcggcagt tggggcgcct tttttcaaaa gttcctccac aacagggagg aattcctcca
    46561 aagcaacggg cttttcgata aaccgagtgg caccgatggt cagcgcaaag gttttgtctt
    46621 caggtgccac ataggtggcc gaaagaaaga ccacgggaat atcacgggtt tcagggttga
    46681 tgcgcaagcg atgcacaagg ctgaaaccat ccattttggg catcagaata tcggtaatga
    46741 caacggcggg acgttccttc tgtatgactt caagagcttc ctgcccgttg ctggcggtga
    46801 cgacgcgata tcccttgaaa acgagggtcg cttccaataa tttgaggata tcggggatgt
    46861 cttctactac aagcagatat tcttgttttg tttccataac tcaattctaa tccattgcgg
    46921 ggtgtctgga catgtcaata tcatgtaaag catacccgtc actcgctaaa ctcaatttga
    46981 aaatacaacc attgggtttc attccctgaa gggaacacca cgtcaatggt attcaccaaa
    47041 gcagagccag tgcgtggaac agagagaccg tacccagaat attcaacttc atgcccgcca
    47101 aggtcaatgc gtatcggctt cgtggggtgt tctccatttt cgtccagtgg atacaacaaa
    47161 cctgaggaac ccaaaatata aaaacgccca tgagaatctt gaaaaatgcg gttgaagtac
    47221 ccagaatctt cggggaggcg ttcatcaaaa agtatctctc ccatttgaga aagaatgcga
    47281 tgcatcaccc agcgttctcc gcctgtggtt tgcccctccc gccaataaag gatatgcata
    47341 ttgccatgcg tatccagata agcatccatt tgtgcgttca aaaaaggcag tggatacgct
    47401 gccgttggag gctcttcatc aaaagagatc aattccaggg gtttgttgag cagatcatct
    47461 gtgcgccaaa atccgatcgc attgaaggcg taatcaaaag aatccgcagg ctgttcatac
    47521 cccaaagcct gccagcggac atcccttgtg gaaaccagag caaactgccc atctgcctgc
    47581 ggaaaaacat aagtgtaaca atatcgaaaa tccaattcac tgatgcggct gacccattgc
    47641 cgatcagcag gcaagtaaca agaccaataa aattccccgc caggttcttc tccgccattg
    47701 cttgctacaa tgcacaacgt accatcagag gcaatgcccg cagaactgta cggccaattc
    47761 ccctgtatca tgttcggaat aacctgcgcc tccattgcaa aagatccgtc aatgggtttc
    47821 cctgcccaaa gtgtggctac accctcaggc cagccgacca gatacaaggt cccatcggga
    47881 gccgcaagca gattgacagg ctcccgccca gcatctccct ccgcaactac gacccagtca
    47941 tttacagcat tccgtctgac caaacgccag acacgatgca attcatcttt tccagaaacc
    48001 gtataagcag tgaacacccc cgcatcggtg cggactacac gggtttgatg tccaccccaa
    48061 atattacctc cgtcacccgt agcagcggcg tcggtaatta actccacctc actttgacga
    48121 atctgtaccg cgctgacaat ggattctgtt tcgaaaggcg tgacagccac ctcaacaggt
    48181 acagttgcgg tgaccgtgat attctcaagc ggcgaagtgg ttggggtggg agaacaggcg
    48241 gcaaacaaaa gataaaacaa aaccttccaa cagccccacg ccttgtaaac aggtctgctt
    48301 ttcattttgt gcggggcaga cgaaatgaga tcaacaattg catcaacaac aagccgatca
    48361 ccaccatccc agtgaccatg atcgcaccca tcaaccccgc attgaattgg atcggtgttc
    48421 gcaaatttgc ataagccgaa atctcgaatg cggtttgaat catcagcgcg atcacgccaa
    48481 acagcatggc tccaaatgaa tttaggcggc caatggtttc ctcccgccgt tcaggagcaa
    48541 gccagaattc acggttgggc atgttgatca ggttgggcgg cagaaccagg aataaaacct
    48601 gcagcggcag gctgactaaa ataacaacca gcaaggtttg ggcttgaaac caaaaaaact
    48661 cggcctttgg agcaaaacga tctgggagac cttgagcatt aaaatgcgca gccatttgat
    48721 ctggggtaat gagccaaagg cgagccattt caaaaaggca gagcaacaat acagcaatat
    48781 aaaaggttct tccgaaacgc atatcacaag ctcctcatgt cctcacgaag attactggcc
    48841 ttcaggaaac agaagcgacc atgaatgttc atcaccagcc aacaacccca aacctgcccg
    48901 ggcaggctgg gcacaagcgt gtgcctgaaa tgccaaagcc acttcggcag cggcaggtga
    48961 aaaataagcg atcacctcac aatgcaaacg accctctgac tcagaacggg taaaaacagc
    49021 catttcaggc ggcttccctg cggagataaa tgcacgttga aaaaatgcct cgatttctgc
    49081 agagggcaca ccagccatga tcccatcacc aagattgagc gaataccagt aattcataac
    49141 tctcccatct gccagccgtc agttcatgcc gctttcccac tcatattgct ccaccaggat
    49201 ccatttggta tccagatgtt tttttaggtg ctggtcaata acgtccatac gtttttgctg
    49261 ctcagcatgg atttgctttt tcgcagggtt cgcgagaaaa ctggccttgt attcatcatt
    49321 caagcggatg aactcctcct tattgaaaat gcctacatga tcaaggtaga gtgagctatc
    49381 aacgctttcc cgaaactcag ggcgggcgtt cctaccaatg taatcgtcca caggccacca
    49441 caaaacataa tccaccaccc aaactgcact gccatcctga actggcaccc ctacgactct
    49501 gactctaaac gccatttcac aattctcctt tgaaaaacac ctgttttcaa ggaacatccc
    49561 tgtttcttga atacctgacg acaagtataa ctctctcgat tcacaatttg agggcaatgg
    49621 ttctggtttt ggggttgctt ctgggtggac gtggcaattc ttcccctcct aacctcccca
    49681 attgcgacga agaacacgtc gaaattgggg agggactgtt gctcttgaaa ttaaaatttt
    49741 agaaacatgc tcaatgttca taaaaactct tcattg
//
LOCUS       DAOQJC010000007        48591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_293_length_48591_cov_10.683019, whole genome shotgun sequence.
ACCESSION   DAOQJC010000007 DAOQJC010000000
VERSION     DAOQJC010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 48591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48591
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_293_length_48591_cov_10.683019"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLE39_01815"
     CDS             complement(<1..564)
                     /locus_tag="PLE39_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNE67087.1"
                     /translation="MKGIILAGGRGTRLHPLTFSMSKQLLPVYDKPMVYYPLSMLMLA
                     EIRDILVISTPEDLPLFRNLLGDGTQWGLKFSYAEQDQPRGLADAFRVGAKFVGNDSS
                     CLILGDNIFFGHGLPEILRRSATLKSGARIFAYPVRDPERYGVVEFDKDGKVLSLEEK
                     PAQPKSNYAVPGVYFYDSQVVEMAANLK"
     gene            complement(561..1640)
                     /gene="rfbB"
                     /locus_tag="PLE39_01820"
     CDS             complement(561..1640)
                     /gene="rfbB"
                     /locus_tag="PLE39_01820"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNE67088.1"
                     /translation="MKNVLVTGGAGFIGSNFVRYLLEADERVHIVNLDALTYAGSLEN
                     LKDLPHAERHTFVQGDICNRDLVRDLLHQHQIDTVIHFAAESHVDRSILSPEPFIQAN
                     VFGTFILLEAARQYWQVEKALPLDEVRFHHVSTDEVYGTLAPDDPAFSETTPYAPNSP
                     YAASKASSDHLVRSYNHTYQLPVTISNCSNNYGPRQFPEKLIPLVILNALTGKQLPVY
                     GDGQQIRDWLFVTDHCEAIHRIVVDGKPGETYNIGGNNQPANLTIVETVCDLLDELQP
                     LAESHRSLIKFVADRPGHDRRYAMNISKIERDLGWKPRHSLTEGLLATVQWILANPDW
                     VSAIQKQREYQTWLAKNYEKREKPS"
     gene            complement(1656..2204)
                     /gene="rfbC"
                     /locus_tag="PLE39_01825"
     CDS             complement(1656..2204)
                     /gene="rfbC"
                     /locus_tag="PLE39_01825"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNE67089.1"
                     /translation="MQFIPTSLPEVILVVPKVYEDERGFFLESYQKERFAAAGISFDF
                     VQDNHSASVQGVLRGLHYQIRQPQGKLVRAVVGEIYDVAVDIRRSSPTFGKWVGAVLS
                     SENRNQLWVPPGFAHGFYVMSGRAEILYKATDYYAPQWERSLLWNDPALGIDWPVTGE
                     NFPILSGKDAQGVLLKDAEIYD"
     gene            complement(2212..2979)
                     /locus_tag="PLE39_01830"
     CDS             complement(2212..2979)
                     /locus_tag="PLE39_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE67090.1"
                     /translation="MTNSNHMPEDVIRLEGVSVLYRKPGEKIRTFKEYAIRILQRRIQ
                     FKEFRALNDINLTIQKGEIFGVVGNNGAGKSTLLKVVSRVLIPTEGRVWIKGVVTPLL
                     ELGAGFHPELTGRENIMLNGTILGHPRREIEEHMDEIIEFSELGDFIDSPLRTYSSGM
                     YARLGFAAATTWEPDILLLDEVLSVGDESFRKKCEARMRGFRNGKTTSIMVSHSMESV
                     MNLCQRVAWLDHGTVRAVGPAVDIVAEYRKAQPGAGA"
     gene            complement(2984..3811)
                     /locus_tag="PLE39_01835"
     CDS             complement(2984..3811)
                     /locus_tag="PLE39_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE67091.1"
                     /translation="MKTSEEFIYDSARRGPVALEELRGIFRYRDLILQLIRKDIVARY
                     KRSVLGVAWTMLQPLGMMIILSVVFSTLFSQVKGYPAYILSGLIAWTFFSQATSAMMT
                     QIVWGGALLRQIYLPRTSFAVSSIGTGLTNLGISFIPLVFIVLIIGLPIRWSILFVPI
                     SMLLLGAFALGVGLIVSTMSIYFPDVSDMYSLVLTGWMYLTPIVYPETLIPEAYRYWF
                     FHLNPMYYYVKIFRAPIFEGVIPDGSTLLVAALISTVTLAIGWLFFSKRSDEFAYHL"
     gene            complement(3948..6515)
                     /locus_tag="PLE39_01840"
     CDS             complement(3948..6515)
                     /locus_tag="PLE39_01840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE67092.1"
                     /translation="MPINMLRWKNKSLTLAVYGLLISVGAFVTAWVVYQVPSDPKNNI
                     FLGLSLQRMILIGSVALAGLLAAVLSVRSFRNETWAEWAWTFLFGRSFLAGLIRWLAA
                     GIAITGWVIWFTPLYRWESNQDYYLRLHPIIVWLTLAGVLTFVVSGVEEYGFDPQNFL
                     STLSSNKKILGIALGVMFAFLLVWAFIALTGMGVWVSDGFWYGAGVPVLSLQIVLAMV
                     IGLGVFALERSPFKARFPKWTDLFIFFLIWGVAAYLWAREPLPTSFFAPGPFLPDYEL
                     HPFSDAATFDRGSQFALIGQGINNGAYWDRALYMAFLVFMHQLFGQNYEHLMNVQAAI
                     YAVFPALLYLLGRDVFNRSFGVILAALVTMRGVNGIMVSTLIDLANQKHMLTDFPTVI
                     FVAWFALMMVRWLKDPARNYLNAVWAGGVVGLGIMLRTNTLFLLVFAGILVPFVYWRQ
                     KLRGAVIGVLILVAMFASTYAWGAYHDKSVFDIYFYRIMIVLKARYPQAAPQLAPEEE
                     PQGNEASPLHVSTVSSTGPRLAEPARVKPVYEFVTIHFLHNIISSVFILPTDDEFHDL
                     WRTVRGDFPYWEPYWDGRFAESAHRFLTLNLLLVALGIAAAWKYSRAPGLAPLGIFFF
                     YDLANAFARTSGGRYVVPIDWIILFYFALGLFQVILWGMAFFGLREETAPVAEQVSWS
                     WEPLKKKAPLIVIFFLFAGSLVPVSGHFIPRRYPERSQEELFALLESQGDIQKMGYDA
                     AELQAAFEQWPELRIVAGRALYPRYFEAGRGIPKNRFPYTAMDFPRIAFTVIGPQGLS
                     YVLLPRADVDYFPNASDVFVLGCGLGANIDALAVVIVDQPNIVYTRRPPVPLQCPVQ"
     gene            6567..7391
                     /locus_tag="PLE39_01845"
     CDS             6567..7391
                     /locus_tag="PLE39_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67093.1"
                     /translation="MPKTSSKKPIGNPTRGKTATNRLRRVDNFILLYEPALLTRTDGL
                     FQHSMFIDLGYGFDARTTLESAERFRRINPSLPILGVEIDKERVEAALPFADSITHFR
                     HGGFNLPLQSGERVRLIRAFNVLRQYEEKDFAPAYERLAEYVLPGGLMIEGTSNPFGS
                     IWVANLVRKMESGWKFEALVFSTNFHMGFDITDFQAVLPKNHIHHVVPGETIYDFFEA
                     WKRSAAETAHAKVFGWKQWFIASAESLAAKGFKIDVHKKWLAKGWLILKNGTSAED"
     gene            7633..8544
                     /locus_tag="PLE39_01850"
     CDS             7633..8544
                     /locus_tag="PLE39_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67094.1"
                     /translation="MASFKKIRLLAVVLLLISMACATPSIFPSNESEINTAVAGTVMA
                     GQTQDAVNELLTPPTIQDTPTLVPTLTFTPEPPTATPTPTVVPTLPFTPTAETPFITV
                     SVDTNCRSGPGKVYDVVGALLVGEFAEVFGRDPSNNYWYILNPDGNDPEFCWVWGKYA
                     TLTGPAMLAPVFTPPPTPTATATSLPTPKFTVEYTSIDSCNGFWWVELKLKNTGGIPF
                     KSVNISIRDKVTDVVVSDTADGFINLDDCQTKSAKEILGIGETFTISTPAFKYNPESH
                     RLFVTITLCSDKGQQGTCATEKIDFEP"
     gene            8561..9376
                     /locus_tag="PLE39_01855"
     CDS             8561..9376
                     /locus_tag="PLE39_01855"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNE67095.1"
                     /translation="MSRSTQAADLNRQFQNKTRTRIRAPKTLFFLLAVMLLVSCISSP
                     EPAAPALTFKNTVVSLTFDDGDADNYAVRDILARNNLRATFYVVSGFTGTNGYMTEDQ
                     LRDLYNDGNEIGGHTLNHSNVPDLSGADLEREICQDRKNLLAHGFEVNSFAYPYGHYD
                     DESKQMVVDCGYSNARGVSGGPDTIPPVDMYALNAMPYIVKDTRFPKMTRYVTEVEND
                     GGGWVIFVFHHVCDGCNEYAIKLSVFDQFAKWLGEQQANGLVVKTMSEVANPK"
     gene            complement(9444..9863)
                     /locus_tag="PLE39_01860"
     CDS             complement(9444..9863)
                     /locus_tag="PLE39_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67096.1"
                     /translation="MKLNSAKEVWVARLIAWVMIGAVSCGIIFVTTFMLGPSMFAVPD
                     LSEKIAVAVFCPGAEGNSRQDGASVPTTSSPSGTYGHTVEITCTFADGSTKTIRNEQY
                     ALASIGGMFGLGGLCGVAISIPLMLLPLFLIRRKKAE"
     gene            10309..10887
                     /locus_tag="PLE39_01865"
     CDS             10309..10887
                     /locus_tag="PLE39_01865"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNE67097.1"
                     /translation="MLDIQTQLFEATDIRFGPIDHETHPEIESKWTHDAEFMRLLDLK
                     PVRPLSPAMIKKQYEAIEKEIEEDKNLFYFTIRAREDDHLIGKAMVEWIDWTNGNGFI
                     RLGIGAAEDRRKGYGSQALHMLLRYAFGELNLYRVTAVVPAYNEGALRLFQKFGFVEE
                     VRRRQALNRDGQFWDLISFGLLNTEWQDQARA"
     gene            10897..11454
                     /locus_tag="PLE39_01870"
     CDS             10897..11454
                     /locus_tag="PLE39_01870"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNE67098.1"
                     /translation="MKDIYRGTLVRLCDESPEIMAKAFAKWDRDTEQHRLADSDPAML
                     LSEKKIKEIVEKRAENKQSAPFSIRTLAEDKLIGGVGLWVHSWSHSDAWLGISIGDRE
                     YWGKGYGTDAMRLMVQYGFIELNLRRISLGLHAYNERARKSYEKIGFKMEGRTRGDGL
                     RDGVRFDSLWMGILREEWLAAQELA"
     gene            11451..12011
                     /locus_tag="PLE39_01875"
     CDS             11451..12011
                     /locus_tag="PLE39_01875"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNE67099.1"
                     /translation="MKDILKGNTVRLSAMNHEEIGQAMSAQARDSELMRLMSTSPSRL
                     HSAKTSVKFFEKMIEKNSPAEYFFSIRALEDDRLLGDINLDVINNWSSRDSFVGLGIY
                     DRADWGKGYGTDAMQITLRFAFTELNLHRVTLTVFEYNPRAIRSYEKVGFKHEGRIRG
                     GLIKDGKRWDMFFMGILHNDWKELQK"
     gene            12008..13000
                     /locus_tag="PLE39_01880"
     CDS             12008..13000
                     /locus_tag="PLE39_01880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE67100.1"
                     /translation="MTQPNLRETLLDTTLKLRTAQWADCEAVAGLIYAVCEADGDTTV
                     AVTPEELKNEWQGEGFNLETDAFVVTTHDDRIIGYEEFFNQHEHYNLRTDGYVHPQFK
                     NLGIGTTLLRRVEARAREEMQLAEPDLRAFLRSTIDSRDAASHELHKAEGYSPKRYHW
                     RMEIKLESAPSPAVFPAGVELRPFIRAEHEVAVWQAQNEAFRDHWGSHDVSFEEFTRS
                     RFDDPEFDPSLWAIAWDGNEVAGFSLNRFRMGIGWVRTLGVRRPWRKKGLGLALLQYS
                     FGELYKRGKDTIGLGVDASSPTGATRLYQRAGMHIASEFLTYEKELRPGRVVED"
     gene            13196..13618
                     /locus_tag="PLE39_01885"
     CDS             13196..13618
                     /locus_tag="PLE39_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67101.1"
                     /translation="MNLRCIYCQTPFTLSRPEMLTAIVTMNEKNLNHYDSHCPRCRRA
                     NSISRQRMELFFPNWQDAAKKQASADAKAAASAASAGVSATAYLKTEPLPKTAKPTAE
                     KKPAAKAAAKKPAAGKSSTAKAKAPAAKKSASTKTKKK"
     gene            13622..14221
                     /locus_tag="PLE39_01890"
     CDS             13622..14221
                     /locus_tag="PLE39_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNE67102.1"
                     /translation="MAIRAVFFDFGGVIMRTEYQSPRQHLAERFGMDYDDIDKVVFAS
                     ESARRASVGAITEDAHWAEVLKRLKHPASEMKSFKDEFFGGDVIDRNLVEFIRSLRGQ
                     VHTGLISNAWSGLREFITSEKVITAFDSVTISAEVGVVKPEGRIYQVALDQAKVKAEE
                     AVFVDDMNINLEGCEDLGMTGILFKNPQEAIEELKRLLA"
     gene            14396..15646
                     /locus_tag="PLE39_01895"
     CDS             14396..15646
                     /locus_tag="PLE39_01895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE67103.1"
                     /translation="MFINLKLDAKKVYLFIEFTASALFAMMFVTMSLYEATVAGLTPL
                     QLVLVGTALEVSAFVFEIPTGVVADVYSRRLSIIIGYFLMGIGFLVEGLFPAFLPILL
                     AQVIWGLGYTFTSGATQAWISDEIGEEEANKLVLRANRVGLYASLLGMGMAVLIGSQS
                     VGLPILFGGAGVVLIGLVLVVIMPETGFHPTPREDRNNWQHMAHTFKEGIKAVRARPR
                     LMTILGIGLLYGLYSEGFDRLWVKHILDTFELPVLFGQTDVAFFGLLRAGSMVLSIFA
                     TRFVEKRIDTGSPLAIGRATLGITFGISAGLIGFALSPIFAVTIIVYWFISVLRNVAG
                     PLYNTWVNQKLDPNTRATVLSMSSQVDAIGQMGGGPLAAAVAGTVSVVAAIITSGLLL
                     LPALPLIGRANSQSIAEKVEQTVS"
     gene            15762..16679
                     /locus_tag="PLE39_01900"
     CDS             15762..16679
                     /locus_tag="PLE39_01900"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNE67104.1"
                     /translation="MDLSKHAFFEGRIVPLADAKINIATHGFLYGTAVFSGMRAYWNE
                     EKKRLFVFRPFDHYQRLLNSARMMAMDIPYDEESLIQLTLDLLRTDNWQQDVYLRPTI
                     YKADMGIGVRLHNLKDEFSMFVMAFEKYVPNDANAHITFSSWRRIDDNSIPARGKIAG
                     AYANSALIKTDANRAGFDEALVLDNNGHVSEGSAMNIFIVRDGVLITPPVTDNILEGI
                     TRRSVIELARNELGLQVQERSIDRTEVFIAEEVFMTGTAAQIVAVTKIDHRPVGIGKM
                     GPITEKLRGLFDEVVRAKLPKYKQWNLEV"
     gene            complement(16758..17171)
                     /locus_tag="PLE39_01905"
     CDS             complement(16758..17171)
                     /locus_tag="PLE39_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67105.1"
                     /translation="MFQVFTGRAALRNIFLALFLLSTIAFVVMAVSTSLVKEAVSEFA
                     PSATTGDDPSSTPPSQDANGMGITAVIGSVVTSVTSLVGFIITTVITWRKEQREASLA
                     EVERKKLELELEKNRLELEELKKGEGKKKEARRKK"
     gene            complement(17190..17924)
                     /locus_tag="PLE39_01910"
     CDS             complement(17190..17924)
                     /locus_tag="PLE39_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67106.1"
                     /translation="MSKYDYNYEATSNPKQSAKLEVWDMLTIATLLLTLCLGVYFVAI
                     FIFPQSAINPLKASMVDPNAPPTATITPLQLDATWTATPFSIVTETPTLLPTHTLIPS
                     PTLFSLITPSDTPTPTPIKAQFSATVTYIDSTIIHPEADCKWQGIGGTIVDANNADIL
                     RITVRLVGSYNGSTKNELTVSSIAPAYGKSGYEFFLGTTPITSRDNLTLQILDQAGLP
                     LSDPIEIDTFKECSKNLVLVRFKKNP"
     gene            complement(17977..18864)
                     /locus_tag="PLE39_01915"
     CDS             complement(17977..18864)
                     /locus_tag="PLE39_01915"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNE67107.1"
                     /translation="MFSKPRFFAQLTLAFVLGLSACASFGTSNTPTPELPTSTPLPPT
                     ATPPPSVATVNGEYITTAEFQAELERYKSAQTALGLSFTEEDAAKAVLEDMISQVLLA
                     QAARAANFTLTEADLQSRIDALAAQLGSADALSQWQSTHGYDDASFRFALTRSIEAAW
                     MRDKIIADVPTVVEQIHVRQILTYNEADARAALDEIKAGADFDEKAAFFDPITFGELG
                     WVPRGYLLDPAADEALFALQPGAYSDVIATDAGFHIFKVVERGDHPLTPDALLVVQER
                     AVINWLADQRAKSDVTLIP"
     gene            complement(18937..20598)
                     /gene="groL"
                     /locus_tag="PLE39_01920"
     CDS             complement(18937..20598)
                     /gene="groL"
                     /locus_tag="PLE39_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920153.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNE67108.1"
                     /translation="MAAKQIVFSEDARRKLKNGMNVVAEAVSATLGPKGRNVAIDRKF
                     GSPTITHDGVSVAKEIELEDPFENMGAQLLKEAAQKTNDIAGDGTTTSTVLAHAIVNE
                     GLKALEAGYNPMLLKKGIELATETIVTELKKNSVKIDTREEIASVATNSAADVEVGEL
                     IADVMDKVGKDGVITVEESKTMQFETEFVEGMQFDRGYLSPYFITNGDQMEAQISDAY
                     VLIYDKKISAAQDIVPLLEKLVQLGKRELVIIAEDVDGEALATLILNKIRGMLNVLAI
                     KAPGFGDRRKAMLQDIASLTGGQVISEETGRKLESTTVQDLGRAEKVVSDKENTTIVG
                     GKGKKGDIEARIKEIRIEMDKSTSDYDKEKLQERLAKLSGGVAIIRVGAATETEMKEK
                     KHRVEDALSAARAAVEEGIVPGGEISLINAAGKLDKLAKAHAEDENEEIKVGINIVKK
                     ALEAPIRKLSSNAGQDGSVIIDTVRRTAAEKKNPNIGYNVLTGEYTDMIKAGVIDPVK
                     VVRGALENAASIAAMILTTDVLITDMPEKDKAPAMPPGGMGGMDY"
     gene            complement(20631..20927)
                     /gene="groES"
                     /locus_tag="PLE39_01925"
     CDS             complement(20631..20927)
                     /gene="groES"
                     /locus_tag="PLE39_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNE67109.1"
                     /translation="MAKLSFKPLGGRVLVEPIEQEEVTAGGIVLPETAKEKPQQGKIL
                     AAGPGDRNDKGERIAMDVKVGDVVLFAKYSGTEVKIEGKKVLILRESDILGIVG"
     gene            complement(21142..22572)
                     /locus_tag="PLE39_01930"
     CDS             complement(21142..22572)
                     /locus_tag="PLE39_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE67110.1"
                     /translation="MNIYQRRDIFRKRGEINIYRMFFWTILILGGIWLIRSVQSGDVK
                     PLFYPTPTPTRFALSYALEGDAQFTAGELDAAITAYGEAAKVDPSNAKLLADLARIQT
                     YSSAMMVSDADTKVRLAEALASAEKAVAISPDDSYAQSVYAFVLDWNANPVLVPDERE
                     VQRLLTKANGAAVRALQLDPNNTRALAFYAEILVDQQKWTNAQQIIDQALERDPSLMD
                     VHRVHAYVLESLGEYALAIEAYDRAILIAPNLTFLHIRAGANYRILAFNSPNEEVQTQ
                     LFEKSLEYFDRAATINDQLGINDPAPYLSIAKTYSQMGQFFIAIRNVQKTIEFQPDKA
                     EFYGELGVLYHKNRNYETGILALKCAIRGCNPDESCEGRGGCGGGDVPAEVSGLPLTS
                     NTVYYYDIYASELAALSTPSNSYCGDALDIADEIEASEFDNDPNIAADIVVVRTICST
                     LNDAPAAGESPAATPETLDVTPTATP"
     gene            complement(22569..23846)
                     /locus_tag="PLE39_01935"
     CDS             complement(22569..23846)
                     /locus_tag="PLE39_01935"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE67111.1"
                     /translation="MYLKGSKWSMNRRRTRPNWFRIILLCLLVLGASYVNRFVIAARP
                     SPFLPTPTVTRSPESFVTEAQEYFKQGQLLRAIDSYKQAILSRPDDPTVYIALAQAQV
                     FAGQYEAAQESAESAILLNNANSMAHAVLAWALGAQEKFIEAEDSIERALTLDPNNAL
                     AHAYYVEILVDSGSFDVIEKAIDESRIAVALAPNTLETHRARGIILEAVGDYENAIRE
                     YQQAIDLNPKIADLYLSLGINYRALGSYEDAITAFSNADALNPEDPTPDFLTSRTYAT
                     RGEFGKAMQYAEQAVANDGADPSLRGNLGVMYYRNGYWTEALVELGYVVDGGFTKDGV
                     QIEAMPLVPNNIRLAEYYFTYGLALARTNKCGEALNIARTILERIPADELSVENANAI
                     ISKCEQNLVQTPEPLPTSPETAPVSTETPTPTP"
     gene            complement(24101..24172)
                     /locus_tag="PLE39_01940"
     tRNA            complement(24101..24172)
                     /locus_tag="PLE39_01940"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24138..24140),aa:Lys,seq:ctt)
     gene            24546..24992
                     /locus_tag="PLE39_01945"
     CDS             24546..24992
                     /locus_tag="PLE39_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67112.1"
                     /translation="MSNSSNGVWKTFFGCGGFSLFLLTVMLLAVVLACSATVAVPIFD
                     RDVLQEIACPPGSTLITGWEETTYTRPGERVLYGYCVDAQGNESPAQDLGYGALEYFP
                     RYFGISLAASLVLTLLVVIPLMILYRVIKKKFFSKPVSTDPAIGSL"
     gene            complement(25042..26460)
                     /locus_tag="PLE39_01950"
     CDS             complement(25042..26460)
                     /locus_tag="PLE39_01950"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNE67113.1"
                     /translation="MTILPLITLIVWSCVLLLVDLFIPKKQKGWTAFLAAFGLVVTLG
                     LTIGQIGQSNTGFSGMVLVDGFSTFANVLLLTSGLFGIALAFGYTRRMGIERGEYYTL
                     LIFSVSGMMLMAQAADLIVVFLALELLSIPLYVLAAFSRANSQSQEAGVKYFLLGAFA
                     TGFVVYGTALVFGATGTTSFSGIFYAVSNGASILLLTIGAALILVGFGFKVAAVPFHM
                     WTPDVYEGAPTAVTAFMSSGAKIAGFAAMLRVFSSAFPALSADISDILWAFAALTMVV
                     GNLTALSQTNIKRLLAYSSIAHAGYILMAFVPYGNSEVSAVSIAAGLFYLVTYALTNF
                     GAWAVVITLEKSEGKGLEINDYAGLAKKYPALAAAMTVFMLSFIGFPPTLGMVGKFYL
                     FRAAVAGGYNGLAIIGVVTSLVSAYYYLRVVVTMYMREGDPTIEREPLLSITTAVTAI
                     ATVVLSFVPQFLFLLSSTALVR"
     gene            complement(26575..28086)
                     /locus_tag="PLE39_01955"
     CDS             complement(26575..28086)
                     /locus_tag="PLE39_01955"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNE67114.1"
                     /translation="MEFLLQPLTLLTFLPLVGVLVILFMNSESKNAIRWVALGTSLIT
                     FVVSLWVFSQFDSSNPDLQLEAKYPWIQVAGWNIYYYLAVDGLSILLVLLTAFLTPIS
                     LLSTWTAVEERVKDFMVFFLLLEVGMMGVFLAQDLFLFYIFWEFTLVPMYFLIGLWGG
                     PRRVYAAVKFFLYTMAGSILMLIAILFLGIKTDTFNVPTMLARLPDLYATSINPQTQM
                     LLFIAFAAAFAIKVPMWPLHSWLPDAHVEAPTAGSVILAGVLLKMGTYGFLRFNIALF
                     PDATVAAAPWIALLATIGIIYGAAVSYAQQDVKKLVAYSSVSHLGFVMLGLFALNAQG
                     VAGAILQMINHGLSTGALFLLIGMIYEQTHTREIKVYGGLWKITPVFGTLMLIASLSS
                     MGLPGLNGFVGEFTILLGAFGSKAIANPWYAGVSAIGVIMAAVYILYMFQKVFLGPAG
                     EITHHHELKDLNWREILTVAPLVIFMFWIGLYPKPFFDILAPAVEKLLSALPL"
     gene            complement(28110..30299)
                     /locus_tag="PLE39_01960"
     CDS             complement(28110..30299)
                     /locus_tag="PLE39_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNE67115.1"
                     /translation="MHLSESVSTGFFFLAPWLVFAPVIGLLINLIFGRQFSEKMVGTV
                     ASLASGTAFVVSVLLAYSVSLNHGEVVRWKLAEWIHVGTLHLDWTFRMDSLSTTMMLV
                     VSGVGTLIHIYAIGYMHEDVRFKHEEGRFNRFFLYLNLFIAAMMILVSGDSYMMLFVG
                     WEGVGLCSFLLIGFWFEMDTLARPSWANSNAAKKAFITNRVGDFGFLIAGFLMFWYLG
                     SFQFDEAFKAAPEIAHSMPWVIVAITLFMLVGVAGKSAQIPLYIWLPDAMAGPTPVSA
                     LIHAATMVTAGVYLVTRSAPLYTLVPQAQYIVAMVGAGTALFAATIAVGQYDIKKVLA
                     YSTISQLGFMVAAVGMGAYVAGMFHLITHAFFKALLFLSAGSVILGLERGHHAHAHHA
                     AHDDHGKKKKEGKHASGKAEGKKEEEHGHGGHDEHGEVFDPGDMRNMGGLRKTMPVTF
                     WLYMIGTLALAGIFPFAGFWSKDEILLDASLHFTSVYWQLTIAAFFTAFYMGRQIWMV
                     FFGEARTDAAKHAEESPKVMTVPLMVLAVLSVAGGALNLPFEGFHNLGHWLGHTIGEV
                     EGLPLDLKVAGISTALALLAILISWFIYGRNPLKAGQIDPLKKPLGFIFTGMEGKWFV
                     DEGYFAVIINPFNKLSQFLADVIDWRFWHDFVHDTVIAGTYNWLSETALNHYADQKGI
                     DGFANWLGTATQSVSATLRKVQNGFVRSYALSVLLGVVLILGYLILK"
     gene            complement(30361..30681)
                     /gene="nuoK"
                     /locus_tag="PLE39_01965"
     CDS             complement(30361..30681)
                     /gene="nuoK"
                     /locus_tag="PLE39_01965"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNE67116.1"
                     /translation="MVPVNYYVALSAVLFTIGALGVLIRRNPLIIFMSVELMLNAGNL
                     AFVAFGRSYEIAGGQPFFSGQIFVFFVIAVAAAEVAVGLALIVEIFKARKNINIDELN
                     SLKG"
     gene            complement(30681..31205)
                     /locus_tag="PLE39_01970"
     CDS             complement(30681..31205)
                     /locus_tag="PLE39_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNE67117.1"
                     /translation="MNSDIIVFLVLSLIAISSALGMLFSRNAVYSALYLVLNFVTVAV
                     FYLLLGAPFIAMSQITVYAGAIMVLFLFVIMLLGAENLAPSQSLPWQKPLAYTLAVVL
                     VVESVYLVISRAQTDKFISTPDAALNSMENLREMAMSLFNQYLLPFEITSILLLVAMV
                     GAIVLTKQEKREQK"
     gene            complement(31276..31797)
                     /gene="nuoI"
                     /locus_tag="PLE39_01975"
     CDS             complement(31276..31797)
                     /gene="nuoI"
                     /locus_tag="PLE39_01975"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNE67118.1"
                     /translation="MGVFDPIIELSKGLGETLRSFLFEKTVTYQYPEEKRPVRPRYRG
                     RHVLKRYDNGLEKCIGCSLCAAACPADAIFVEAAENTDEKRFSPGQRYASTYEINMLR
                     CIYCGFCEDACPTEAIVLGDNYELSFTDRRQAIYPKEMLLEPVRTVDMITPRKVEPGV
                     FTRSVPEMQNPSD"
     gene            complement(31784..32923)
                     /gene="nuoH"
                     /locus_tag="PLE39_01980"
     CDS             complement(31784..32923)
                     /gene="nuoH"
                     /locus_tag="PLE39_01980"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNE67119.1"
                     /translation="MDWTIWLEWVLKGFILCLILLTGFAYLTLYERRALARMQVRVGP
                     NRAGPQGVLQPIADAVKLIFKEEFTPSRVYKVVFVLAPVLTVVPSLILAAVIPLGSKF
                     VLFGREITLYVADLNVGVLYLTSIASIAVYGIVLAGWSSNNKYAMLGGVRSSAQMISY
                     ELSLGLCIVIAILLGDSMNLNDIVNAQKGMWFAVIQPAGALVFMIVTLAEVNRAPFDM
                     PEAEQELTAGYHAEYSGMKFALFFMAEYQKMIVICMIAATLFFGGYREFWFLQGTIFS
                     VDRFWFLGPIYLLLKVIVLLFFMIWIRATWPRIRYDRLMSFGWKVLLPLSLILAFITA
                     TGILLAQELNNPMIIWGIPVLSIVCVLVAVVFINSDLRRKSHGRI"
     gene            complement(32925..35393)
                     /gene="nuoG"
                     /locus_tag="PLE39_01985"
     CDS             complement(32925..35393)
                     /gene="nuoG"
                     /locus_tag="PLE39_01985"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNE67120.1"
                     /translation="MTKQVTLSINGITVTVPDGTLIADAAKMAGIDIPVFCHHPKLEP
                     AGMCRMCLVEVGRPMLDRATGQVVMENGQPKIQFMPKLETACTNRVSDGMVVMTTTDK
                     AKDGQKGTVEFLLTSHPLDCPICDKGGECPLQNLTMAFGPGKSRFIYDEKAHFEKQVP
                     LGDLIWLDRERCIQCARCIRFQDEVAGEAVLGFDERGRNTQIISLSEPGFDSIFSGNT
                     TDICPVGALTTADFRFGARPWELKAQASICTQCPVGCNTTLNTRREAKAGGDVVVKRV
                     MPRQNEEVNEIWLCDKGRFTYHFTESKKRLTRPLVRKEEKLARASWDAATKMAAENFT
                     KSKKDFVVLASGRLANEDLFNLKSLADTAGGTAILYSNMGGGDLTTLVGVGQGTNIGK
                     MGKGDAILVVASDLYQEAPIWWLRVKQAADRGATLIVANARETKLDKFAKFVIRYAQG
                     DESKAVANLSNKGKIADEFAKAKNAVVFYGNDGLGLNDTASLASACATLLKDTEHVGK
                     PNNGLVAVWERVNDQGAWEIGFRPEPDLEKALKGKAVYIVGADPVGDDPALAKALKGA
                     KFVAVQDILETATTEIADVVFPAQAYTEREGTFTSGERRVQRFYAAVPVKGDAKPDFA
                     ITSQIAKQMGVILEGSSASVVFDILSHSVESFADLSYAKLAETHGQWPIVGRGDLYYG
                     GTTYENTQGLGVQLVPTAQAGGTVRIPKVRKEAALRPKENELLAVPVTRLYDYGVTIS
                     SSAPLLGQWVGEPTVTLHPMAAKNLGVEAGTRVKLSFDGVNAEVVVKLDDTISAGVAL
                     VPRSMGIAIREPVVARVQLAGKVK"
     gene            complement(35404..36651)
                     /gene="nuoF"
                     /locus_tag="PLE39_01990"
     CDS             complement(35404..36651)
                     /gene="nuoF"
                     /locus_tag="PLE39_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNE67121.1"
                     /translation="MANVLLRHRDIVDLNKIEVYKKNGGFDAFKKAVSKMQPNEVTDV
                     VKNSGLRGRGGAGFPTGMKWSFIDTKNWPHYVVANADESEPGTFKDREIMESNPFQFL
                     EGLAIASYAVGANVAYIYLRGEFWQVAAFLDEKIADMEKAGFLGENLFGTEYSLKIYT
                     HLGAGAYICGEETALLESLEGKRGQPRVRPPFPPSFGLYGKPTIINNVETFTNVPLIM
                     ANGAEWYKSMGTADSAGVKIFSLSGRVRKPGNYELPFGTTFRQLIYEHGGGVLDGRPV
                     KAIMPAGASSSLIVVDDKVLDTPMDYATVRTLGADLGSASVIVLDDTVDIDWVVNKTI
                     HFFKHESCGKCTTCREGNYWMQHLTERIHSGQGVQNDVDLLLNVAKQMQGKCLCALGE
                     FSTMAVVTGIERFPQDFKKAVKA"
     gene            complement(36651..37157)
                     /locus_tag="PLE39_01995"
     CDS             complement(36651..37157)
                     /locus_tag="PLE39_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNE67122.1"
                     /translation="MSLEKSYPKEVKQILSKYPPEHKRSAVMPLLYLAQREEGYVNKA
                     AMQDIARMLDITETEVASIVGFYSLYHDEKAGKYRMQICTDLPCALRGADDFMKNLCS
                     NLGIKVGETTPDGLVTLEAVTCLAACDKAPMFQTQGPDGIKYHENMTVDKTIELIEAL
                     KKSDGGVK"
     gene            complement(37154..38383)
                     /gene="nuoD"
                     /locus_tag="PLE39_02000"
     CDS             complement(37154..38383)
                     /gene="nuoD"
                     /locus_tag="PLE39_02000"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HNE67123.1"
                     /translation="MSQIEEVSLEKFKHLVSERALTGETMLLNMGPQHPSTHGVLRLL
                     LELDGEIVVNCIPDIGFLHTGVEKNMEAKTYQKAEVMTDRLDYMNTMGNNLAYCMAVE
                     KLVDLDVPVRAQALRVILVELQRIASHLVWLGTSGLDLAAMSMFLYCFREREQILDIF
                     ELVSGQRMMTTYFRPGGVWRDVPVEFEKAVRDFIKIFPKRIAEYESLLTKNPLFIDRM
                     VDIGYLSKETALSYGVTGPTLRASGVDWDLRKVRPYCGYEQYDFNVPTRTEGDTYARY
                     LVRIEELHESLKIVEQALNKLPLGPVRSENRKFVPPPRSEIGVSMEALIHHFKLWTEG
                     FSAPQASIYNAVESPRGELGLFIEGDGGPKPLRMHLRTPSFDNLAVVPQIVKGHLVAD
                     LVAILASIDIVLGDIDR"
     gene            complement(38386..38898)
                     /locus_tag="PLE39_02005"
     CDS             complement(38386..38898)
                     /locus_tag="PLE39_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNE67124.1"
                     /translation="MNKNLEPVVKTIQDKFGAGCEEFRDEVHLFVKPEQIVEALTLLR
                     DEQGFELLSAYTAVDYYPSETPRFHLIYQLTSLVKNLSVQVRVAVNGSSPKVPTVTGV
                     YASANWREREIFDMFGIEFEGHPDPRRILMPEDHVGHPLRKDFPLGYEEPQFTFNFDE
                     IDLRKPYVKE"
     gene            complement(38953..39441)
                     /locus_tag="PLE39_02010"
     CDS             complement(38953..39441)
                     /locus_tag="PLE39_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNE67125.1"
                     /translation="MGVEQKLGNMGVVTTTLENVVNWSRTNAMWPMLFGLACCAIEMM
                     AAQASDYDMSRFGMELMRASPRQSDLMIVAGRVSKKMGPVLRRLYDQMPEPKWVIAMG
                     DCASCGGVFNNYAIYQGVDEVVPVDVYVAGCPPRPEALIHGVLTVYEKVKGEKFKDLA
                     AK"
     gene            complement(39432..39788)
                     /locus_tag="PLE39_02015"
     CDS             complement(39432..39788)
                     /locus_tag="PLE39_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNE67126.1"
                     /translation="MLLEYIAIGVMIAVATLIAFIAIGLGELFGPKKKTAAKDMPYES
                     GMNPYGEGTRRMPVRFYLIAVLFILFDIEVVFFLPWAIAFRKLGIFGLLEMIVFIVIL
                     LIGYVYAWKKGALEWE"
     gene            complement(39999..40847)
                     /locus_tag="PLE39_02020"
                     /pseudo
     CDS             complement(39999..40847)
                     /locus_tag="PLE39_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(41277..41525)
                     /locus_tag="PLE39_02025"
                     /pseudo
     CDS             complement(41277..41525)
                     /locus_tag="PLE39_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012367.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
     gene            complement(41653..43557)
                     /gene="dnaG"
                     /locus_tag="PLE39_02030"
     CDS             complement(41653..43557)
                     /gene="dnaG"
                     /locus_tag="PLE39_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNE67127.1"
                     /translation="MSTIDEIKARIDIVDLVSEAGVKLRHAGKNYTGFCPFHDNKHTP
                     AFVVWPESGTWRCFGQCNEGGDIFKFVMKRDGLDFKEAMQKLALRAGVEIKEYQRETP
                     QQKEAYDHLRKLLEDAVVYYRTQLFGAPAILAYLHEKRGLTDSTIETFGLGYAPPGYD
                     NLLKHFLSKGYSQQDLIDSGMVTVRENDEARTYDKFRNRIMIPIRDENGKMAGFGARI
                     VDPNDVPKFLNSPETPIFSKGRLLYGLDRARKPIRAADQAVIVEGYLDVIALHQAGYE
                     NVVSPMGTALTEDQLRLLKRSTRRIVLALDPDAAGQKAVLRGLDAARSAMDREGELGF
                     DARGLLKNEARLQADLRVATMPDDLDPDEIVARDQEEWKKLIENAKPIVTHVMDSLAA
                     GQNLNDAKVKNQIAAQVLPLIEDLPSAMERDTYRQALARMLRVNESVLTGTQVQGPVA
                     RRKPRSVEQSHKVVSSGIVAVNPTLKIESYCLSVLLRKPELLYRLDKRMQEADLAALA
                     VEDFEYTDHQLLFGVVRMAVEQDEKDYHHYVMAHVPESVSELSKELVARSDALDPVED
                     KILDDLLARFMDLRRANAMLNVNQLRFLQEEDQQQGGANMKLYQEQTLQMTRLLHSLD
                     QAKRKLSSKR"
     gene            43587..>45149
                     /locus_tag="PLE39_02035"
     CDS             43587..>45149
                     /locus_tag="PLE39_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67128.1"
                     /translation="MNSRLIKNKVFALPFLAVYAWLVIRTSWVGDDAIITFRSLENFL
                     HGYGPVFNIGERVQTFTHPLWFLLQAAAGAVFNLWKSNPFGQGQMYFLNVFISIGISL
                     LAAAIFTFNETSSAKGAALGLLIFSVSKSFVDYSTSGLENPLTHLILLLFTLTYVGPA
                     KNSQRRLYLLATLAALGGVDRLDTLLFYFPALVLLFWESPHKGKTFLTLGFGFLPLIA
                     WELFSLFYYGVPFPNTAYAKINTGIPEASLFMQGLFYYLNSLKLDPITLLTIFTVSAA
                     GWMSKNKKHQAILAGVLLYLLYTIYIGGDFMSGRYFSSPLLVCVTLLATMDFESPKKF
                     GVAVGIVLLVGLAPIAVVAERAPAFGVGDEGDHRVFVDEHGISDERIVYTGLGLFERL
                     KKKSAPSTNYARDKWVYLPDEPVRVRIVGPLGLNSMTLGPNFHVIDLNSLADPLMTRM
                     PLYEIDRWRIGHFRHIIPKGYEETLKSGENRIVNKDIAQYYDKLSFIVRGELWSWERV
                     VEIWNFNTGKYDY"
     assembly_gap    45150..45249
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(45473..47023)
                     /locus_tag="PLE39_02040"
     CDS             complement(45473..47023)
                     /locus_tag="PLE39_02040"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNE67129.1"
                     /translation="MADQNNGLEYIEQLLRDGKKQEGRTALVQYVQSHPDSSRAWWML
                     SFILTDPKQQLDCVERVLKLDPGNIPARNRLLKLQSDLAPKSDSTFSQEPSRLHPITA
                     PFILEDESAPQTSSDAFEASFLRQSQGNVRPPEAATFLPDDSIDDRDAFNISNSSYQA
                     AQDNYKPSPFFPEDDLDLQSAPPLSAFERSYSNTPSQDPYQSSSIPPLFLPEDEDLPR
                     PSADAFEHSFGQDAYENPYSSSVSPFQTETQSADKPAQPARKKPAPKKKNDFVKYIVL
                     IVVFLCVFGLILVGFFFVLRGQQISTPTAQPIFLGPTETPTTIPTIGLPPTWTPAPSP
                     TLIPTSTLGYIESTSTPISNVIGTEDPNAETQVGLGTGMAAPDFTLKNVNTGNQVSLS
                     DYKGKPVVIFFWANGCGYCDTEAAAMQDIYTQYKDIGLVVLAVDVWGDSDHARTYRDN
                     HNLTYPMLIDSKASTFKKYGGTNLFPVNYFVDVNGNISFGQVGMMDYSMLNMNVRRLL
                     NLIPTVSP"
     gene            complement(47194..>48591)
                     /gene="glyS"
                     /locus_tag="PLE39_02045"
     CDS             complement(47194..>48591)
                     /gene="glyS"
                     /locus_tag="PLE39_02045"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNE67130.1"
                     /translation="LVGGQAIIEEGLLNEVVNLIEMPTAVMGGFNEEFLALPRDVLIS
                     VMKKHQRYFPVQRVGATLQSQSDTRSDLGRSPLLAKFIAIRNGDDIHIDTVRQGNEHV
                     LGARFADANFFVREDVKLKLEEYRPKLSSLTFHTKLGSMLDKSERIVKLVNELIPSLG
                     LEADQAIFARRAAHLMKADLVTQMVTEMTSLQGIIGGEYALRSGEQKEVAAAISEQYQ
                     SVPQTKAGLTIALADRLDSLVGMFGAGLAPTGAKDPFGLRRAAIGVVMPLMSPAVGMS
                     PMDFDLAKAVKLSAQTQPIPVSDEVQKQILEFITGRMKVALGDMGFKHDVIEAVLAAQ
                     SNNPAGTVRAVDQLSAWVEREDWSSILDGFARCVRITRDQKETFKVSEKSLVEEAEKG
                     LYEAVNRLPPVVAGDVDSFLNNVSALIPAITAFFDKVLVMVEDKAVKENRLGLLQKIA
                     GLSNGIADLSKLEGF"
BASE COUNT        12316 a        12779 c        12472 g        10924 t
ORIGIN      
        1 cttgagattg gcagccattt ccacaacttg cgagtcgtaa aaatacacac ccggcacggc
       61 atagttcgat tttggctggg cgggcttttc ttccaggctc aacaccttgc catctttatc
      121 gaattcaacc acgccgtaac gttcggggtc acgcacaggg taggcaaaga tgcgtgcacc
      181 ggatttcaac gtggccgagc ggcgcagaat ttcaggcaaa ccgtgaccga agaaaatgtt
      241 atcgcccagg atcaggcagg atgaatcgtt tccgacaaat tttgcaccga cgcggaacgc
      301 gtctgccaac cctcgcggct ggtcctgttc tgcgtaggag aatttcagcc cccactgagt
      361 cccgtcaccg agcagattgc ggaaaagcgg caggtcttcg ggggtgctga tgacaagaat
      421 atcgcgaatc tccgccagca tcaacatcga aagcggataa tacaccatgg gtttgtcgta
      481 caccggcagc aattgcttgc tcatggaaaa ggtcagcggg tgcaggcgtg taccgcgtcc
      541 accggctagg atgattccct tcatgatggc ttctctcttt tttcgtagtt ttttgccagc
      601 caggtctggt attcacgctg tttttgaatg gcactcaccc aatcggggtt ggcaaggatc
      661 cactgcaccg tggcgagcag accttcggtc aaagaatggc gcggcttcca gccgagatcg
      721 cgctcgatct tgctgatgtt catggcatag cggcggtcgt gaccgggacg gtctgccacg
      781 aatttgatca aactgcgatg cgactcggcc agaggctgaa gttcatccaa caggtcacaa
      841 acggtttcga caatggtaag gttggcgggc tggttattgc cgccaatgtt gtaggtttca
      901 ccgggtttgc catccacaac gatcctgtgg atcgcttcgc aatggtcggt cacaaacagc
      961 caatcgcgga tctgctgtcc atcaccatac acgggcaatt gcttgccggt caacgcattg
     1021 aggatgacca gcggaatcaa tttctcaggg aactgacgcg ggccataatt attcgagcag
     1081 ttggaaatgg tcaccggcag ctgataggtg tggttgtacg agcgtacaag atgatcgctg
     1141 gatgccttcg aagcggcata gggcgaattc ggcgcatagg gggtcgtttc agaaaaagca
     1201 gggtcatcgg gcgcaagcgt accgtacact tcatcggtgg aaacatgatg aaagcggacc
     1261 tcatcgagag gcagagcctt ctccacctgc caatactgtc gtgccgcttc aagcaggata
     1321 aaggtgccaa acacattcgc ctgaatgaac ggctccgggc tcaaaatcga acggtcaaca
     1381 tgtgactcgg ccgcaaaatg gatgacggtg tcgatctggt gctggtgaag caaatcgcgc
     1441 acaagatcgc ggttacaaat atcgccctgc acaaaagtat gccgctctgc atgcggaagg
     1501 tctttcaaat tttcgaggct gcccgcgtag gtcaacgcat cgagattgac gatatgaacc
     1561 cgttcatccg cctctaaaag ataacgtaca aaattggagc cgataaaacc cgccccgcca
     1621 gtgaccaaaa catttttcat tttgtctccc ggagtctaat cataaatctc agcgtctttg
     1681 agaagcacgc cctgcgcatc cttcccggac agaattggga aattctcacc tgtcacgggc
     1741 caatcaatgc caagagcagg atcattccaa agcagactgc gttcccattg cggagcgtaa
     1801 taatctgtag ccttgtaaag gatttcagcc cgcccgctca tgacgtaaaa cccatgcgcg
     1861 aatccgggcg gaacccaaag ctggttcctg ttttctgacg aaaggaccgc cccgacccat
     1921 tttccaaacg tcggggaact gcggcggatg tccacggcca cgtcgtagat ctcaccaacg
     1981 accgcccgca ccagtttacc ctgaggctgg cgaatctggt aatgcaatcc gcgtaaaact
     2041 ccctgcaccg aagccgagtg gttatcctgc acaaaatcaa atgaaatgcc tgccgccgcg
     2101 aaacgttcct tctggtagct ttccaaaaag aagccgcgct catcttcata cactttcggc
     2161 acgaccagaa tcacctcagg caggctggtt ggaataaact gcatgtagat tctacgctcc
     2221 tgcgcccggt tgtgccttgc gatattccgc cacaatatcc acggcggggc ccactgcacg
     2281 aactgtacca tgatccagcc aggcaacacg ctgacaaaga ttcatcaccg attccatgga
     2341 atgtgaaacc atgatggagg tggtctttcc attgcggaaa ccgcgcatac gcgcctcaca
     2401 cttcttacgg aaggattcgt caccaaccga aagcacttca tcaagcagga ggatatccgg
     2461 ttcccaggtg gtggcggcgg caaacccgag gcgggcgtac atgccgctcg aataagtgcg
     2521 caacggcgaa tcgataaaat cacccagttc agagaattcg atgatctcat ccatgtgctc
     2581 ttctatttcg cggcgcggat gaccgaggat ggtgccattc agcatgatgt tctcgcggcc
     2641 ggtcaactca gggtgaaagc ccgcgcccaa ctccaacagc ggcgtgacaa cccctttgat
     2701 ccacacccgt ccttcggtcg ggatcaagac ccgcgaaaca accttgagca gggtgctttt
     2761 cccggcgccg ttattgccca ccacaccgaa aatctcccct ttttgaatgg tcaggtttat
     2821 atcgttcagc gcgcgaaatt ccttgaattg aatccgccgc tgaagaatgc ggatggcata
     2881 ctctttaaag gtgcggatct tttcccccgg cttgcgatac aacacggaga ccccttcaag
     2941 gcggataaca tcttctggca tgtgattcga attggtcatt ggtttacagg tgataggcaa
     3001 attcatcgga gcgtttcgag aagaacagcc acccgatggc aagagttaca gtagagatca
     3061 atgcagctac aagcaaagtg ctcccatccg ggatcacccc ctcaaagatc ggcgcgcgga
     3121 aaattttcac gtaatagtac atggggttca aatgaaagaa ccagtagcgg taggcttcag
     3181 ggatcagggt ttcagggtaa acgatgggcg tcaagtacat ccagccggtc aacaccaggc
     3241 tgtacatatc cgatacatca gggaagtaaa tggacatggt ggagacgatc aaccccacac
     3301 ccagggcaaa cgcccccagc aacagcatcg agatcggcac aaaaagaatg ctccaacgaa
     3361 tcggcaggcc aatgatgagc acgataaaca caagcggaat gaacgaaatg cccaaattgg
     3421 tcaatcctgt gccgatcgaa gaaacggcaa aagacgtgcg cggaagatag atctgccgca
     3481 acaaagcgcc gccccaaacg atctgggtca tcatggcgct ggtggcctgt gaaaaaaacg
     3541 tccacgcgat caaaccgctg agaatatagg ccgggtatcc cttgacctga ctgaacaagg
     3601 tcgaaaagac caccgacaga atgatcatca tgccaagggg ctggagcatc gtccaggcaa
     3661 cgcctaaaac cgagcgcttg taacgtgcca cgatgtcctt gcggatcaat tgcagaatca
     3721 aatcacgata tcgaaagata ccgcgcaatt cttccaacgc caccggtccg cgccgggcgc
     3781 tatcataaat aaactcttca gatgttttca taccactcac aaaaggtaac taaattccaa
     3841 aaaactgacc caaaaccagg gccagaacaa aaatccatgg tgtcgatttt tgaacgggat
     3901 taaaacagcg ttttcgattc taccatacgg aaaaaccgca gaaacggcta ctgcacaggg
     3961 cattgcaaag gcaccggcgg acggcgcgtg tacacaatgt tcggctggtc tacaatgacc
     4021 acagccagtg catcaatgtt ggcccccaac ccacacccca aaacgaacac atccgaagca
     4081 ttcgggaaat aatccacatc ggctcgcggc agcaacacat aactcaaccc ctgcgggccg
     4141 atcaccgtga aggcaattcg ggggaaatcc atggcggtgt acgggaagcg attcttcgga
     4201 atgcccctgc ccgcctcaaa atatcgtgga tacaaggccc gcccggcgac gatcctgagc
     4261 tcaggccatt gctcgaaagc cgcctgcaat tccgccgcgt cgtatcccat cttttgaata
     4321 tcaccctgcg attcaagcag tgcgaacaat tcctcttgtg aacgctcggg gtaacggcgc
     4381 ggaatgaaat gacccgacac cggcaccagc gaccctgcaa acaggaagaa aatgacgatc
     4441 aacggagctt ttttctttaa cggttcccaa ctccacgaaa cctgctcagc gacaggagca
     4501 gtctcttccc gcaggccaaa gaaagccatt ccccacaaaa tgacctgaaa caagcccagc
     4561 gcaaaataga acaggatgat ccaatcaatg ggcacgacgt agcgtccgcc ggaggtgcgg
     4621 gcaaaggcat tggcaaggtc gtagaaaaag aagatgccca acggcgccag ccccggagcg
     4681 cgggaatatt tccaggcggc ggcaatgccc agcgcgacca gaagcaggtt caaggtcagg
     4741 aaacgatgcg cgctttcagc gaagcgtcca tcccaatatg gctcccaata cggaaagtcc
     4801 ccgcgcaccg tgcgccacag gtcgtggaac tcgtcatcgg ttggcagaat aaagaccgat
     4861 gaaataatgt tatgcaggaa atgaatggtg acgaattcat aaacaggttt cacccttgca
     4921 ggttcagcca ggcgcggacc cgtggacgat acagtggaaa catgcagcgg gcttgcttca
     4981 tttccctgcg gttcttcttc gggtgcaagc tgcggcgccg cctgcggata ccgcgcctta
     5041 agaacgatca tgatcctgta aaaataaata tcgaaaacag atttatcgtg atatgccccc
     5101 caggcatatg tgcttgcgaa catcgcgacc aaaatcaaca cgccgatcac tgcgccgcgc
     5161 aatttctgcc gccaatagac gaagggaacc aagatccctg cgaacaccaa aaggaaaagc
     5221 gtgttcgtgc gcagcatgat tcccagcccc actacaccgc ctgcccacac ggcgttcagg
     5281 taatttctgg cggggtcctt cagccagcgc accatcatca aagcgaacca tgccacgaaa
     5341 atgaccgtcg ggaaatctgt gagcatgtgt ttctggttgg cgagatcgat caaagtgctg
     5401 accatgatcc cattcacgcc gcgcatcgtc accagtgccg caaggatcac accaaatgag
     5461 cggttgaaca catcccgtcc cagcaaatat agaagtgccg ggaataccgc atagatcgcg
     5521 gcctgcacgt tcatcaggtg ttcgtaattc tgtccgaaca attgatgcat aaataccaaa
     5581 aaggccatgt acagggcgcg gtcccagtag gcgccgttgt tgataccctg accgatcaac
     5641 gcgaactgac tgccgcggtc gaaggttgcc gcatcggaaa atggatgcaa ttcataatca
     5701 ggcagaaacg gtcccggcgc aaagaaactg gtgggcaatg gttcacgcgc ccaaagatac
     5761 gccgccacgc cccagatcaa aaagaaaatg aaaaggtctg tccattttgg aaaccgcgcc
     5821 ttgaacggcg aacgttccag tgcgaacaca ccgaggccga tcaccatcgc cagcacgatc
     5881 tgcaaactca gcacaggcac acccgcgcca taccaaaagc cgtcgctcac ccataccccc
     5941 atgccggtca atgcaatgaa ggcccaaacc aaaagaaaag cgaacatcac gcccagcgca
     6001 atgccaagga tctttttatt cgatgaaagc gtgctcaaaa agttctgcgg atcaaaacca
     6061 tattcttcca cccctgaaac gacaaaagtc aaaaccccgg cgagggtcag ccaaacgatg
     6121 atgggatgca gacgcaggta ataatcctga ttgctttccc aacgatacaa aggcgtgaac
     6181 caaatcaccc aacctgtaat ggcgattccc gccgccagcc agcggatcag ccccgcaaga
     6241 aacgaccgcc cgaagagaaa ggtccacgcc cactctgccc aggtctcatt gcgaaacgag
     6301 cggaccgaaa gcaccgccgc cagcagccct gccagcgcaa cactgccgat cagaatcatt
     6361 cgttgcagcg acagccccaa aaaaatattg ttcttcgggt cagacgggac ttgatacacc
     6421 acccaggccg tcacaaacgc gccgaccgag atcagcagtc catagaccgc cagggtgagg
     6481 gatttgtttt tccagcgcaa catattaatc ggcatgagtt taccataacg gaaccccatc
     6541 aatttcgccc gtataattgg gcatgcatgc cgaaaacttc aagcaaaaaa ccgattggaa
     6601 acccaacccg cggaaaaacc gccaccaacc gactccgccg tgtcgataac tttatcctgc
     6661 tctatgagcc agcgcttctc acacggaccg acggcctgtt tcagcattcg atgttcattg
     6721 atcttggcta cggcttcgac gcccgcacca ccctcgaaag cgccgaaagg ttcaggcgca
     6781 tcaacccgtc gctgcccatc ctcggcgtgg agattgacaa ggagcgggtc gaggccgcgc
     6841 tgccctttgc cgacagcatc acccactttc ggcatggagg atttaacttg cctttgcaat
     6901 caggcgaaag ggtaaggctg atccgcgcgt ttaatgtcct gcgtcaatat gaagagaagg
     6961 attttgcgcc tgcgtatgaa cgtctggctg aatatgtgtt accgggcggg ctgatgatcg
     7021 aaggcacgtc gaatccattt ggcagcattt gggtggcgaa cctcgtacgc aaaatggaaa
     7081 gtggatggaa atttgaggca ctcgtgttca gcaccaactt tcacatgggg tttgacatca
     7141 ccgattttca agccgtactt ccgaagaatc acatccacca tgttgtgccc ggtgaaacca
     7201 tttatgattt tttcgaagcc tggaagcgct ccgcagctga gaccgcccat gcaaaagtct
     7261 tcgggtggaa gcaatggttc atcgcctcag ccgaaagcct ggcagccaaa ggattcaaga
     7321 tcgatgtgca taaaaaatgg ctggccaaag gctggttgat cctgaaaaac ggaacaagcg
     7381 cagaagattg agcgtcaggt taacgcacca gacgggtaga attctcttaa ttaatagaag
     7441 catcctctcg tcatgcaatt ctcgtgaaat aaggtgtgac gcattaaatc agtttttctc
     7501 taccgtacat ttgcaccgcg aagcccgcaa agggcgcgaa gaaatcttta aaacctggcg
     7561 atcttggcgt gcttcgcggt aaaaatcgag aaatgtacgg cagcaagatc agtttcaaaa
     7621 aaggaaagtc tcatggcatc tttcaagaaa atccgtttac tggctgtggt gctgttattg
     7681 atcagcatgg cttgcgccac gccatccatt tttccatcga acgaaagtga gatcaacact
     7741 gccgtggcag ggacggtcat ggcgggccag acccaggatg cagtaaacga actcctcacc
     7801 ccgcccacca tacaagacac ccccaccctt gtccctacgc tgacattcac acccgaaccg
     7861 cccacagcaa cgcccacacc cacggtggtg ccaactttgc cattcacccc cacagcagag
     7921 actcctttca tcaccgtgtc ggtggataca aactgccgca gcggcccagg gaaggtatat
     7981 gatgtggtcg gcgccctgct ggtgggagaa tttgccgaag tgttcggccg tgacccaagc
     8041 aacaattact ggtacattct caaccccgat ggcaatgacc ccgagttttg ctgggtatgg
     8101 ggaaaatatg ccacactgac agggccagcc atgctcgcgc cggtcttcac accgccgccc
     8161 accccaaccg ccacagccac atccctgccc acgccgaaat tcaccgtcga atacaccagc
     8221 atagattcgt gcaatggctt ttggtgggtg gaactcaaat tgaagaacac cggcggcatt
     8281 ccgttcaaat cggtgaacat ttcgatcagg gataaagtca ctgatgtcgt tgtctccgac
     8341 actgccgatg gatttatcaa tctcgatgac tgccagacga aaagcgcaaa ggaaatcctt
     8401 ggcattggcg aaactttcac catcagcaca cccgccttca aatacaaccc tgaaagccac
     8461 agactcttcg tcaccattac attgtgttcg gataaaggtc aacagggaac atgtgcaaca
     8521 gaaaagatcg attttgagcc gtaattacaa aggagatgaa gtgtcacgat caacacaggc
     8581 agcagacttg aacaggcaat tccaaaacaa aacgcggacc aggatccgcg ctccaaagac
     8641 gctgtttttt ctattggcag tgatgctttt ggtcagttgc atcagcagcc ctgaacctgc
     8701 cgcgcccgca ctcactttca agaataccgt ggtcagcctg accttcgatg acggtgacgc
     8761 cgacaactac gccgtacgcg acatcctcgc aaggaataac ctgcgcgcca ccttctacgt
     8821 cgtcagtgga tttaccggca cgaacgggta catgaccgaa gatcaactgc gcgacctgta
     8881 taacgatggc aatgaaatcg gcggtcacac cctcaaccat agcaatgtcc cagacctgag
     8941 cggtgcagac ctcgaacgcg agatctgtca ggatcggaaa aacctgctcg cgcatgggtt
     9001 cgaggtgaat tccttcgcct atccctacgg ccactatgac gatgaatcaa agcaaatggt
     9061 ggtggattgc ggctacagca atgcgcgcgg agtctccggc ggcccggata ccattccgcc
     9121 cgtggatatg tatgccttga atgccatgcc ttatattgta aaagacaccc gctttccaaa
     9181 aatgacccgc tacgtcaccg aggtggaaaa cgatggcggc ggctgggtca tattcgtttt
     9241 tcatcacgtc tgcgacgggt gcaacgaata cgcgatcaaa ctcagcgtgt tcgaccagtt
     9301 tgcaaaatgg cttggcgaac aacaagccaa cggattggtg gtcaaaacga tgagcgaagt
     9361 ggcgaatcca aaataggcca cactgtaacg tgaatttttc aaagtcggct tcaaagtctg
     9421 tggattttga agccgacttc gtttcactcc gcttttttcc tgcggatcag gaataacggc
     9481 aaaagcatca gcggaatgga aatcgccacc ccgcataaac cacccaaccc aaacatgccg
     9541 ccaatggaag ccagcgcgta ttgttcattg cgaatggtct tcgtactgcc atcggcaaag
     9601 gtacaagtga tctccactgt gtgtccatac gtacccgatg ggctggatgt ggtcggcaca
     9661 gatgcaccgt cctggcgtga gttgccttcc gcgcccgggc aaaagactgc cacagcgatc
     9721 ttctcactta aatcaggcac agcgaacatt gaaggtccca gcatgaatgt ggtgacaaaa
     9781 atgatgccac acgaaaccgc accgatcatc acccacgcga tcaaacgcgc cacccaaact
     9841 tcctttgcag aattcaattt catgactcac ctctaatcat ttttcgcgga aacacacaat
     9901 taacaatgat tgtatcgaat ccaaacagca gatacaacct cccaaagttt cactgccgcg
     9961 aaatttcacg gcgatcggcc gcagtgataa aagtacaata tttcccaaaa cccttgactc
    10021 tattttcctc cgcgtgataa actcaccacg cttcaaaaaa gcatattcca tgaaaggagg
    10081 cgcacgccta atgataatga acaagtttta cacctttatc gctcccatcg gtaacaccga
    10141 tgtcgattct cgcagtgcgc ctgcccgcag ttagacaaac tttccaagtt gtcatgcggc
    10201 cacggtgtca ctgctgaccc gtggccgttt ttatttaatc gcgtgaaagg cggccatggg
    10261 tccatgagac ctgtggccgt ttttttgttt tttggtttgg aggaaaaaat gttggatata
    10321 caaacacagt tattcgaagc aacggatatc cgtttcgggc ccattgacca tgaaacacat
    10381 cctgaaatcg aatccaaatg gacgcatgac gccgaattca tgcgcctgtt ggacctgaaa
    10441 cccgtccgcc cgctctcacc tgccatgata aagaagcagt atgaggccat cgaaaaagaa
    10501 atagaggagg ataagaacct cttctacttc accatccgcg cccgtgaaga tgatcatttg
    10561 atcgggaagg ccatggtcga gtggatcgac tggacgaacg gaaatggttt tattcgcctt
    10621 ggcattggcg cagccgaaga ccgccgcaaa ggctatggct cgcaggcatt gcatatgctg
    10681 ttacggtatg cctttggtga gttgaacctg tatcgtgtta cggccgtcgt ccctgcttat
    10741 aacgaaggcg cgctacgcct cttccagaag tttggttttg tggaggaagt ccgcaggcgg
    10801 caggcattaa accgtgatgg gcaattttgg gacctgatct ctttcggcct gctgaatacc
    10861 gaatggcagg atcaggcccg ggcataggag gacgcgatga aagacatcta tcgcggcacc
    10921 ctggtccgcc tgtgcgatga atcgccggag atcatggcca aggcattcgc aaaatgggat
    10981 cgcgacaccg aacagcatcg tctggcggat agcgatcccg ccatgctgct ttcggaaaaa
    11041 aagatcaagg aaatcgtcga aaaacgcgcc gagaacaagc aatcggctcc gttttccatt
    11101 cgcaccctgg ctgaagacaa gttgatcggc ggggtggggc tatgggtaca ttcttggtcc
    11161 cactccgatg cctggctggg catttccatc ggcgaccgtg agtattgggg caaagggtat
    11221 ggcaccgatg ccatgcggct tatggtgcaa tatggattca ttgagttgaa tctgcgccgc
    11281 atttcgctcg gcctgcatgc ctacaatgaa cgcgcccgaa aatcctacga gaaaatcggg
    11341 tttaagatgg aaggccgcac acgtggcgac ggccttcgcg acggagtgcg cttcgacagc
    11401 ttgtggatgg gcattctgcg tgaagagtgg ctggcagcac aggagttggc atgaaagaca
    11461 ttctcaaagg aaatactgtc cgcctctctg ccatgaacca cgaagagatc ggtcaggcca
    11521 tgtccgcaca agcacgcgac tcggaactga tgcgtttgat gagcaccagc ccgtcccgtc
    11581 tgcattctgc caaaacctcg gtcaagttct tcgaaaagat gatcgagaaa aattcgcccg
    11641 ccgaatattt cttcagcatt cgcgcactgg aagatgaccg tcttttgggt gatatcaatc
    11701 tcgatgtcat caacaactgg agctcacgcg actcatttgt tgggctgggc atctacgatc
    11761 gcgcagactg gggcaaaggt tatggaaccg atgccatgca gatcacactt cgttttgcgt
    11821 tcaccgaact caacctgcac cgcgtcaccc tgaccgtatt tgaatacaac ccgcgtgcaa
    11881 ttcgttccta cgaaaaagtg ggcttcaaac atgaaggccg catacgcgga ggtcttataa
    11941 aagatggaaa gcgttgggat atgttcttca tgggcattct ccacaacgac tggaaggagt
    12001 tacaaaaatg acacaaccta atttacgaga aacgcttctt gatacgaccc tgaaactgcg
    12061 cactgctcaa tgggcagact gcgaagctgt tgcaggactc atctacgccg tgtgtgaagc
    12121 cgatggcgac accactgtgg ccgtcacacc agaagaattg aaaaacgaat ggcagggtga
    12181 aggtttcaat cttgaaaccg atgccttcgt tgtcacaact cacgatgacc gtatcatcgg
    12241 ttacgaagaa ttcttcaacc agcacgaaca ctacaacctg cgcaccgatg gatacgtcca
    12301 cccgcaattt aagaaccttg gcatcggcac caccctttta cgccgagtcg aggcgcgcgc
    12361 ccgtgaagaa atgcagcttg ccgaacctga cctgcgggcc tttctgcgca gcaccataga
    12421 cagccgcgat gccgccagcc atgaactgca caaagccgaa ggctattctc ccaaacgcta
    12481 ccattggcgc atggagatca aactcgagag cgccccatct cctgccgtat ttcccgcagg
    12541 tgtggagctt cgtccgttca tccgcgccga acatgaggtg gcggtctggc aggctcagaa
    12601 cgaagccttc cgcgatcact ggggcagcca cgatgtatcc ttcgaagaat tcacccgcag
    12661 ccgcttcgat gaccccgaat ttgatcccag cctttgggcg atcgcctggg atggaaatga
    12721 agtggcgggc ttttcactta accgcttccg catgggcatt ggctgggtcc gcacattggg
    12781 agtccgccgc ccgtggcgca aaaaaggcct tggccttgcg ctgctccagt attcatttgg
    12841 cgaactctac aaacgcggta aagataccat tggccttggg gtggatgcct ccagccccac
    12901 tggcgcaacc cgcttatacc aacgggcagg catgcacatc gccagtgaat tcctcacgta
    12961 cgaaaaggaa cttcgccccg ggcgcgttgt ggaggattaa tttacactca aaaaagaccc
    13021 tgatggccta aaaacgagcc aattggaggc ttgaatagac aaaaaaggcc ttcagggtct
    13081 atagtttctg gccgtttcac tgccacaaaa aatggaaatg aaaagccggt tcgcccggcg
    13141 ggtgaaatca gtgtagaatc aaagcatatt cgtgtttttg gaaaggataa tccctatgaa
    13201 cctgcgttgt atttactgcc agaccccatt cacccttagc cgccccgaga tgctgactgc
    13261 cattgtgaca atgaatgaaa agaacctgaa tcactacgac agccattgcc cgcgttgccg
    13321 ccgcgccaat tccatctcgc gtcagagaat ggaactgttc ttcccgaact ggcaggatgc
    13381 cgccaaaaag caggcttctg ctgatgccaa agcggcggct tcggctgcca gcgctggagt
    13441 ttcggccacg gcgtatctta aaactgagcc tcttccaaaa acggcgaaac ccaccgccga
    13501 gaaaaaacca gcagccaagg ctgctgccaa gaaacctgcc gcaggcaaat cttccacggc
    13561 aaaagccaag gccccggccg cgaagaaatc tgcaagcaca aagaccaaaa agaagtaacg
    13621 catggccatc cgcgcagtat ttttcgattt cggaggcgtg atcatgcgca ccgagtacca
    13681 gtcaccgcgc caacatttgg cggagcggtt cggcatggac tacgatgaca ttgacaaggt
    13741 cgttttcgcg agcgaatctg cgcggcgtgc ctcggtaggt gctattactg aagatgccca
    13801 ttgggccgag gtgctgaaac gcttgaaaca ccccgcttcg gagatgaaat ctttcaaaga
    13861 tgaattcttc ggcggtgatg tgattgaccg caatctggtg gaattcatcc gctctttgcg
    13921 ggggcaggtc catacagggc tgatctccaa tgcatggagc ggattgcgtg agttcatcac
    13981 cagcgaaaag gtcatcactg ccttcgattc ggtcacgatc tcggcggagg ttggtgtggt
    14041 caagcctgaa gggcggatct atcaagttgc gttggatcag gccaaagtta aggcagagga
    14101 agcggtcttc gtcgacgata tgaatatcaa cctcgaaggc tgtgaagacc tgggcatgac
    14161 cggcatcctg ttcaaaaatc cacaggaagc cattgaggaa ctgaaaaggc tgctggcata
    14221 gtttttgcaa cacaggttcc acattttcga taaagttttg aaacttcagc gtctaaccca
    14281 cgctgttgtc ttttaaggag tatgcgatga aaatcatcat cttttcaaac actgactggc
    14341 aaacggagtt cgctctccgt gcctgatgac tggcttttat taaaaaagga atagcatgtt
    14401 tatcaattta aagttggatg caaaaaaagt atatctattc attgaattta cggcatcggc
    14461 acttttcgcg atgatgttcg taacaatgtc gttgtatgaa gcaacggtgg cagggctgac
    14521 cccgttgcag cttgtattgg tgggaactgc gttggaggtc tcagcttttg ttttcgaaat
    14581 tccaacaggc gtggtcgcag atgtgtactc gcgccgactt tccatcatca tcggttattt
    14641 tttgatggga atcggttttt tggtggaagg gctcttccct gcctttctgc cgatcctgct
    14701 ggcacaggtg atttgggggt tgggatatac ctttaccagc ggagctacac aagcctggat
    14761 ctcggatgaa attggcgaag aagaggcaaa caagctggtg ctgcgcgcca accgcgttgg
    14821 gctgtacgcc tccctgcttg gaatgggaat ggcggttttg atcggctcac aatcggtggg
    14881 gttgcccatt ttatttggcg gggcaggtgt ggttttgatc gggcttgtct tggtggtcat
    14941 tatgcctgag accggtttcc accccactcc cagagaagac cgcaacaact ggcagcacat
    15001 ggcgcatact ttcaaggaag gcatcaaggc tgtccgtgcg cgcccgcgcc tgatgaccat
    15061 tctgggaatt gggctgttat atggtttgta tagcgaaggc tttgaccgtt tgtgggtaaa
    15121 acacattctt gataccttcg aattgcctgt cctcttcggg caaaccgatg tagccttctt
    15181 tggtctgctg cgcgcaggga gcatggtcct ttccattttc gcaacccgat tcgtggagaa
    15241 gcgaatcgat acgggcagtc cgctggccat tgggcgtgcc actctgggaa ttaccttcgg
    15301 catctctgcc gggctgatcg gctttgcgct ttcgcccatt tttgccgtga cgatcatcgt
    15361 ctactggttt atcagtgtcc tgcgaaatgt ggccggtccg ctttacaaca cgtgggtgaa
    15421 tcaaaaactc gaccccaata cacgcgccac cgtactttcc atgtcttcac aggtggatgc
    15481 cattgggcaa atggggggcg gaccgctggc agcggcagtg gctggaactg tttctgtggt
    15541 tgccgcgatc attacatcgg gactgctgct actgccagcc ctgccattga tcggacgggc
    15601 caattcacaa tccattgcag agaaggttga gcaaaccgtt tcataaacgc gggggttagt
    15661 taattcataa taaaagggaa gctggattct tttcgatccg gcttcccgat tgaatggatt
    15721 ataattttca ggcaatcacc aattcaaatc ggaggtgtct agtggatctc tcgaagcacg
    15781 ctttctttga gggaagaatc gtcccacttg cggacgcaaa aatcaacatt gccacacatg
    15841 gctttttata tggaaccgcc gtgttcagcg gtatgcgggc atattggaac gaagagaaga
    15901 aacgtctctt cgtttttcgt ccatttgacc attatcagcg ccttttaaac tcagcccgca
    15961 tgatggccat ggatattccg tacgacgaag agagtctgat acaacttacc cttgaccttt
    16021 tgcgcacaga taactggcaa caggatgtat acctgcgtcc caccatctac aaggcagata
    16081 tgggcattgg ggtacgcctg cacaacttaa aagatgagtt ctccatgttc gtgatggctt
    16141 tcgaaaaata tgtccccaat gatgcgaatg ctcacatcac tttctcatcc tggcggcgta
    16201 ttgacgataa ctccatacca gcgcgcggga agatagcggg agcatatgcc aactctgctt
    16261 taatcaagac cgacgccaat cgcgcaggct tcgatgaagc tctggtgctc gataacaacg
    16321 gccatgtttc tgaaggctcc gccatgaaca tcttcattgt ccgtgatggg gttctgatca
    16381 ctccacccgt caccgataac atcctcgaag ggatcacccg ccgttcggtt attgaactgg
    16441 cacgcaacga actagggctg caagtacaag aacgttccat tgaccgcact gaagtgttca
    16501 ttgctgaaga agtgttcatg acaggcaccg ccgcccagat cgtagctgtg acaaagatcg
    16561 accaccgccc ggttggcatc ggaaaaatgg gacccatcac agaaaaactg cgcggtttgt
    16621 tcgatgaggt tgtccgcgca aaactcccca aatacaagca gtggaacctg gaagtttaga
    16681 gaaaaggtac aaagtttctc atgaaaaaag gccagccttg tgaggctggc ctttttgatt
    16741 tattcacata aaaacggtca cttcttcctg cgtgcttcct tctttttccc ctctcctttt
    16801 ttcagttcct caagttccaa tcggttcttt tccaattcca attcaagttt tttacgttcc
    16861 acttcagcca gtgaagcctc ccgttgttcc ttgcgccatg tgatgaccgt ggtgataata
    16921 aaacccacca gcgaagtgac ggaagtgacc acagaaccaa tgaccgcggt aatgcccatt
    16981 ccattggcat cctgcgaggg cggggtcgaa gatgggtcgt caccggtggt cgccgatggg
    17041 gcaaattcac tgaccgcttc ctttaccaag gaagtgctga ccgccattac cacaaacgca
    17101 atggtggaaa gcaggaacaa agccagaaaa atgttgcgaa gggcggcccg cccggtgaat
    17161 acctgaaaca cggaagcact cccgaaaaat tacggattct tcttgaaacg taccaacacc
    17221 agatttttcg aacattcctt gaatgtatcg atctcgatcg ggtcggaaag gggcaggcca
    17281 gcctgatcga ggatctgcaa agtcaggtta tcacgggatg tgatcggggt ggtacctaaa
    17341 aagaactcat atcctgattt tccataagct ggcgcaatac tgctgacggt caactcgttc
    17401 ttggtgctgc cgttgtaact ccctacaaga cggacggtga tcctgagaat atcggcattg
    17461 ttcgcatcca caattgtgcc gccaatgccc tgccatttac agtcggcttc agggtggatg
    17521 atcgtactat caatgtaggt tactgtagcg gaaaattgag ccttgatcgg cgtcggagtg
    17581 ggagtatcag aaggagtgat aagagagaaa agggtcggcg aagggatcaa tgtatgcgtg
    17641 ggaagcagag tcggtgtttc ggtcacaatg gagaaaggag tggcggtcca ggtggcatcc
    17701 agttgcagcg gtgtgatcgt cgctgtgggc ggcgcatttg ggtcaaccat gctggccttc
    17761 aaaggattga tggccgactg ggggaaaata aagatcgcga caaaatagac gcccaaacaa
    17821 agcgtcagca aaagcgtcgc aattgtcagc atatcccaga cctcaagttt tgcgctctgc
    17881 tttgggttgg aggtcgcttc atagttataa tcgtatttgc tcattactta gctccaatga
    17941 caacttatct cactgcaaca acgggttgca tgcaaactag ggaattaaag taacatcact
    18001 ctttgcgcgc tgatcagcaa gccagttgat gactgcacgt tcctgaacga ccagcaaggc
    18061 atcaggagtc agcgggtggt cgccgcgttc aacgactttg aaaatatgaa atcccgcatc
    18121 tgttgcgatc acatctgaat aagcgcctgg ctgaagggca aacaacgcct catctgccgc
    18181 cgggtcgagc aggtaacctc gtggcaccca acccaattca ccaaacgtga tcggatcgaa
    18241 aaatgcagcc ttctcatcga aatctgcacc ggctttaatt tcatccaaag ctgcgcgtgc
    18301 atccgcctca ttataggtca aaatctggcg aacgtggatt tgttccacaa ccgttggcac
    18361 atccgcaatg attttgtcac gcatccatgc tgcttcaatg gaacgggtca acgcaaagcg
    18421 gaaggaagca tcgtcatacc cgtgggtgga ttgccactga cttaaagcgt cggcactgcc
    18481 taattgagct gccaacgcat caatccttga ctgtaagtct gcctctgtta aagtgaaatt
    18541 cgctgctctg gcggcttgtg ccagcaaaac ctgcgagatc atatcttcga gcacagcctt
    18601 ggccgcatct tcctctgtga aggagagtcc caaggcagtt tgggcagact tgtatcgttc
    18661 caattccgcc tgaaattcag cggtggtaat gtactcacca ttgaccgtcg ccacggaagg
    18721 cggtggagtg gcagtgggag gcaaaggtgt ggaagtaggc aattcgggag tgggcgtgtt
    18781 cgaggtcccg aacgaagcgc aggcacttaa tcccaaaaca aaagccagag tcaattgagc
    18841 aaaaaaacgg ggtttggaaa acattcgtgg aattatatat taaaaaagta gggacacggc
    18901 gttgccgtgt ccctacaaat ttgtttatgg gcaggtttag taatccatgc cgcccatgcc
    18961 gccaggaggc atggcaggag ccttgtcctt ctcgggcatg tcggtgatga gcacgtcggt
    19021 ggtgaggatc atcgcggcga tggaagcggc gttttcgaga gcgccacgaa ccaccttcac
    19081 ggggtcgatc acaccagcct tgatcatgtc ggtgtattcg ccggtgagga cgttgtaacc
    19141 gatattcgga ttcttcttct cggcagcggt gcggcggacg gtgtcaatga tgacggagcc
    19201 atcctgacca gcgttggaag aaagcttgcg gatcggggct tcgagcgcct tcttgacgat
    19261 attgatgccg accttgattt cttcgttctc atcttcagcg tgagccttgg cgagtttgtc
    19321 gagtttgcca gcggcattga tgagggagat ttcaccacca gggacgatgc cttcttcaac
    19381 ggctgcgcgg gcagcggaga gagcatcttc aacgcggtgc ttcttttcct tcatttcagt
    19441 ttcggtcgcg gcgcctacgc ggatgatggc aacaccaccg ctgagcttgg caaggcgttc
    19501 ctgaagtttt tccttgtcat agtcgctggt gctcttgtcc atctcaatgc ggatttcttt
    19561 gatgcgggct tcgatatcac ccttcttgcc tttgccgcca acgatggtgg tgttttcctt
    19621 gtcggaaacg accttctcag cgcggccgag gtcctgcacg gtggtggatt cgagcttgcg
    19681 gccggtctct tcagagatga cctgtccacc ggtgagggag gcgatatcct gaagcatggc
    19741 cttgcggcga tcaccaaagc caggagcctt gatggcgagc acgttgagca tgccgcggat
    19801 cttgttgagg atgagggtgg caagagcttc gccgtccaca tcttcagcga tgatgacgag
    19861 ttcgcgcttg ccgagctgga cgagcttctc gagcaaagga acgatgtcct gagcagcgga
    19921 gatcttcttg tcgtagatca aaacatacgc atcgctgatc tgggcttcca tctggtcgcc
    19981 gttggtgatg aagtaggggg agagataacc gcggtcgaac tgcatacctt cgacgaattc
    20041 ggtttcgaac tgcatggtct tggattcttc aacggtgatg acgccgtctt taccgacctt
    20101 gtccatcaca tcagcaatga gttcaccaac ttccacatca gccgcagagt tggtcgccac
    20161 agaagcgatt tcttcgcggg tgtcgatctt cacggagttc ttcttgagtt cagtaacaat
    20221 ggtctctgtg gcgagctcaa tgcccttctt gaggagcatc gggttgtagc cagcttcgag
    20281 agccttgagg ccttcgttca cgatggcgtg cgccaaaacg gtggaggtgg tggtaccgtc
    20341 accggcaatg tcattggtct tctgagcggc ttccttgagg agctgggcgc ccatgttctc
    20401 gaaaggatct tcgagttcga tttccttcgc aacggaaaca ccgtcgtgag tgatggtggg
    20461 ggagccgaac ttgcggtcaa ttgccacatt gcggcctttg gggccgaggg tagcagaaac
    20521 agcttctgca acaacgttca tgccgttctt aagcttgcgg cgggcgtctt ctgaaaatac
    20581 gatttgttta gcagccataa ttcacttcct ttgaatttct gtttggtgga ttaaccgacg
    20641 ataccgagaa tatcgctctc gcgcagaatg aggaccttct tgccctcgat cttcacttcc
    20701 gtgccggagt acttggcgaa aagcaccaca tcgccaacct tcacatccat ggcgatgcgc
    20761 tcgcccttgt cattgcggtc gccagggcca gccgccaaaa tcttgccctg ctggggcttt
    20821 tccttcgcgg tttcgggaag aacgataccg ccagcggtca cttcttcctg ttcgatgggc
    20881 tcaaccaaaa cgcggccacc caggggttta aatgagagtt ttgccatttc ttgcctccat
    20941 taagagattt gaattttttt gaataagcac agaattagca ctcaatactt gcgagtgcta
    21001 attgcaatat tacctactta aattggtcaa gtcaagggga ttgtaaaaat tcttacaaaa
    21061 ctctgatgga tgaaaagccc gcctctcccc tcactgataa ctgaatctgt ttcaaacggc
    21121 attctcgccc cgctaaattg gttacggggt cgcagtgggc gtcacatcca atgtttctgg
    21181 cgtagccgca ggggactccc ccgccgcggg agcatcattc agcgtggaac agatcgtccg
    21241 caccacaacg atatccgcgg caatgttcgg gtcattatca aattccgaag cttcgatttc
    21301 atcggcaata tccaatgcgt caccgcaata gctatttgaa ggcgtactca aagccgccaa
    21361 ttcagaggca taaatatcat aataatagac cgtattcgaa gtaagcggca atccgctcac
    21421 ctccgcaggg acatcaccgc ctccgcaacc tccgcgccct tcacaggatt catcggggtt
    21481 gcatccgcga atggcgcatt tcagggcaag gatacccgtt tcataattac ggttcttgtg
    21541 atacagcacc cccaactcgc cataaaactc ggccttatca ggctggaatt caatggtctt
    21601 ttgcacattg cggatggcaa taaaaaactg acccatctgc gaataggttt ttgcgatcga
    21661 aagatatggt gcagggtcgt tgatccccaa ctgatcgttg atggtggcag cccgatcaaa
    21721 gtactccagt gatttttcaa aaagctgggt ctgtacttcc tcgtttgggc tgttaaaggc
    21781 aaggatacga taattggccc ctgcccgaat gtgcaggaaa gttaaattgg gagcgatcaa
    21841 tattgcacgg tcataggcct caatggcaag cgcatactct cccagcgatt caagcacata
    21901 cgcatggact cgatgcacat ccatcaatga tgggtcacgc tccaacgcct ggtcaatgat
    21961 ttgctgggca ttggtccact tctgctggtc caccaaaatt tcggcgtaga atgccaaagc
    22021 gcgtgtgttg ttgggatcca attgcaaggc acggacggcg gcgccattgg ctttagtcaa
    22081 aagacgttgc acttcccttt catcaggcac aagcaccggg ttggcgttcc agtccagcac
    22141 aaaggcataa acagactggg cgtaactgtc atcgggtgaa atggcaactg ccttctccgc
    22201 cgatgcaagc gcctcagcca gacgcacctt ggtatcggca tcgctcacca tcatggctga
    22261 agaataggtt tggatgcgtg ccaaatcggc cagcaatttg gcattggagg ggtctacttt
    22321 tgcggcttca ccgtaggctg tgatggcggc atccaattca cctgcagtaa attgagcatc
    22381 cccctcaagc gcataggaca gtgcaaaacg cgttggggtt ggtgtgggat aaaacaaagg
    22441 ttttacatcg ccgctctgca ctgaacggat caaccagatt ccgccaagga tcaggatggt
    22501 ccaaaagaac atgcggtaga tattgatctc cccccgcttc cgaaagatgt cacggcgctg
    22561 gtaaatgttc atggtgtcgg ggtgggggtt tctgttgaaa caggagcagt ttcaggcgaa
    22621 gtgggaagcg gctccggcgt ctgtaccaga ttctgttcgc acttactgat gatcgcgttg
    22681 gcattttcca ctgatagttc atcagcagga atgcgctcca gaattgtgcg ggcaatattc
    22741 aaggcttcac cacatttgtt ggtgcgggca agcgcgaggc catatgtaaa atagtattct
    22801 gcaagacgta tgttgtttgg cacaagcggc atcgcctcga tctgaactcc gtcttttgta
    22861 aagccgccat cgaccacata acccaattcc acaagcgctt ccgtccagta gccgtttcga
    22921 taatacatta cgccaaggtt accgcgcagg ctggggtcgg caccatcatt ggcaacagcc
    22981 tgttccgcat attgcatggc ttttccaaat tcaccgcggg tggcataagt gcgtgaagtg
    23041 agaaaatcgg gggtggggtc ttcagggttc aaagcatcgg cgtttgaaaa tgcggtaatt
    23101 gcatcttcat aactgccaag tgcgcggtaa ttaattccca gtgaaagata caggtcggcg
    23161 atcttcgggt tgaggtcaat ggcttgctga tattcgcgga tggcattttc gtaatcgccc
    23221 acagcctcaa gaataattcc gcgtgcgcgg tgggtttcga gggtattggg cgccaaagcc
    23281 acggcaattc gtgattcgtc aattgctttt tcaatgacat caaaggaacc cgaatccacc
    23341 aggatttcga cataatatgc gtgcgccaat gcattgttgg ggtcaagggt cagggcacgt
    23401 tcaatgctgt cttccgcttc gataaacttt tcctgggcac ccaaagccca ggccagcaca
    23461 gcatgcgcca tggaatttgc attgttaagc agaatcgcac tttccgcgct ttcctgtgcg
    23521 gcttcgtact ggccagcaaa cacctgcgcc tgcgccagtg caatatacac cgtggggtca
    23581 tcaggacggg aaaggatggc ttgtttgtaa gaatcaatcg cccgcagtaa ttgtccctgc
    23641 ttgaaatatt cctgtgcctc tgtgacaaat gattcaggag aacgtgtgac tgtgggggtg
    23701 ggcaaaaacg gcgatggccg tgcggcaatg acaaatcgat tcacgtagga cgcgcccagc
    23761 accaacaaac acaacaaaat tatgcggaac cagtttggac gggtccgcct gcgattcatg
    23821 ctccacttgc tgccttttag atacatcgtt ttttatatta ccattttcat gaaatcaacg
    23881 tcaagcaggg ggtacgttct ctcatttgaa tctaagcgga tttatggact ggatgccccg
    23941 aaaaccccat ctttttgaag acacaatcgt tacacgaagt cagcgtttcg accttaactc
    24001 acaaatggtt taaacagtcc aaacacaacc tcccccgtaa acactctcag aaagataaaa
    24061 gaaaaaggtc tgactttatg aaatcagacc taatccatag tgactccgtt gggatttgaa
    24121 cccaaaacca attgattaag agtcaactgc tctgccgttg agctacggag ccgctctcaa
    24181 aacttgcgag cggtattata ccgcaaagaa aaggggtgtc aataaaaaag ttgactagcg
    24241 gtatggcttt ctcaaagtcg ggagtacttg cccaacatag ataaaactaa ccgctaagag
    24301 cgcaaagagc gcgaagttta atggattttc ttggcgttct tcgcgggctt ggcggttcga
    24361 aaggcttgtc agatattaat ttgagaaagc cataagacta ccgtttgcat ggggcactcc
    24421 atacgttgat cagcctgaaa cgcttcacct gaccttcatc ccatttcatt gaagtgaaaa
    24481 acaacctaca atctttttat ctaaggcctg cggtataagt cattccccta ttaagaaggt
    24541 gtgatatgtc aaatagctct aatggcgtct ggaaaacctt ttttggctgt ggcggattct
    24601 ccttgttttt gcttacagtg atgttgttag cggttgtttt agcttgcagc gcaacggtgg
    24661 ctgtccccat ttttgaccgg gatgtcctgc aagaaatcgc atgtccacca ggcagcaccc
    24721 tcattaccgg ttgggaggaa accacctaca cccgccccgg cgaaagggtc ctttacgggt
    24781 attgtgtgga cgcacaaggt aacgaatcac ccgcacaaga tctaggctac ggcgcattgg
    24841 aatatttccc caggtatttt ggcatctctc tcgcagcgag tttggtattg acccttcttg
    24901 tggtcattcc gctcatgatc ctgtatcggg ttatcaagaa gaaattcttc tcaaaacccg
    24961 tttccactga ccctgctatt gggagtttat aataaaaaaa gcgatgactt gaaggtcatc
    25021 gcttttttgt tgaaatcaag cctatcttac cagtgcagta ctcgacaaca ggaacaagaa
    25081 ttgagggaca aagctcaaaa cgacagttgc aatggctgtt acagcagttg tgatgctgag
    25141 caaaggctcg cgctcaatgg tcgggtcacc ttcacgcatg tacatggtga ccaccacacg
    25201 caggtagtaa tacgctgaaa caagcgatgt caccacaccg atgatggcaa ggccgttgta
    25261 tccgccagca actgcggcgc ggaacaggta gaattttccg accatgccaa gggtgggcgg
    25321 gaaaccgatg aaggagagca tgaacacagt catggcggca gcaagggcgg gatatttctt
    25381 tgcaagaccg gcataatcat tgatctcgag ccccttgcct tccgattttt caagggtaat
    25441 gaccacagcc catgcaccga aattggtcaa tgcataggtg accagataaa acaggccagc
    25501 cgctattgaa acagcagaga cctcggaatt cccgtaaggc acaaaagcca taaggatgta
    25561 cccggcatga gcaatacttg aatatgccag caggcgctta atgttggttt gggataaggc
    25621 ggtcaggtta ccaaccacca tggtcaacgc ggcgaacgcc caaagaatat ccgagatatc
    25681 ggcggaaagg gcgggaaaag cagaggagaa aacgcgcaac atggcggcaa accctgcaat
    25741 ctttgcgcct gaagacatga acgctgtcac agcagtgggg gcaccttcat atacatccgg
    25801 agtccacata tggaagggaa cagcagcaac cttgaagccg aaaccgacaa ggatgagagc
    25861 agccccaatg gtcagaagca ggatcgaggc tccgtttgaa acggcgtaaa agattccgct
    25921 aaaggatgtg gtccctgtcg ctccaaaaac gagagccgtt ccgtagacaa caaatcctgt
    25981 tgcaaatgct cccaacagaa aatatttaac tcccgcctcc tgagattggg aattggcccg
    26041 tgaaaaggct gccagcacat acaacggaat ggacaaaagt tcaagcgcaa gaaaaacgac
    26101 gatcaggtcg gcggcctgcg ccatcagcat catgcctgag acgctaaaaa taagcagggt
    26161 gtaatattca ccgcgctcaa tacccatacg acgggtgtat ccgaaggcca gtgcaattcc
    26221 aaatagtccg ctggtgagca gcaacacatt ggcaaaggtt gaaaagccat ccaccaacac
    26281 catgccgcta aagcctgtat tgctttgccc gatctgaccg atggtaaggc caagtgtaac
    26341 aacaaggcca aaggcggcaa gaaatgccgt ccaacccttc tgctttttgg ggatgaacag
    26401 atccaccagc aaaagcacac atgaccaaac gatcagtgtg atcagaggga gaatagtcaa
    26461 gtaatcctga taattaggtt gcgccatgaa cgctccaaga aaatgcaggt tgcagaccgc
    26521 aggttgaaag ttacaggtat acacctttgg ctttcaacct gccaaccgtc aaccctataa
    26581 aggcaatgca gataaaagtt tctcaaccgc aggggcgaga atgtcgaaga atggtttggg
    26641 gtaaagaccg atccagaaca tgaaaatgac caacggagcc acggtgagaa tctcgcgcca
    26701 attgaggtct ttgagttcgt ggtggtgggt aatctcgcca gcagggccga ggaatacttt
    26761 ttggaacatg tacagaatat acacagccgc catgatgaca ccgatggctg aaacacccgc
    26821 ataccacgga ttggcaatgg cctttgaacc aaacgcgcca agcaaaatgg tgaactcacc
    26881 cacgaagccg ttcaaaccag gcaggcccat cgaagaaagc gaagcgatca gcatgagtgt
    26941 gccaaagaca ggggtgatct tccacaaacc gccgtagact ttgatctcgc gggtatgggt
    27001 ctgttcgtag atcataccga tgaggaggaa tagcgcacct gtgcttaacc cgtggttgat
    27061 catttgcaaa atggcacccg ccacaccttg ggcattcaac gcgaacaagc caagcatcac
    27121 aaagccgagg tggctcacgg atgaataggc gaccaatttc ttaacatcct gttgggcata
    27181 ggaaacagct gcgccgtaaa tgatgccaat ggtcgcaagc aacgcaatcc acggggcggc
    27241 agcgactgtc gcatccggga agagcgcaat attgaagcgc aggaacccat aggtacccat
    27301 cttcagcaaa acgccagcca ggatgacaga accagcggtg ggcgcttcca cgtgggcatc
    27361 gggcagccac gaatgcagcg gccacattgg cactttgatg gcaaaagccg cagcaaaggc
    27421 aatgaagagc agcatttgag tctgcgggtt gatgctggtg gcatacaggt caggcagacg
    27481 ggcaagcatg gttgggacgt tgaaggtgtc ggtcttgatg ccgaggaaca aaatggcgat
    27541 cagcatcaaa atggaaccag ccatggtata gaggaagaac ttgaccgcag catatacgcg
    27601 gcgaggtccg ccccacagac cgatgaggaa gtacatcggg acaagggtaa attcccagaa
    27661 gatgtagaac aggaacagat cctgggcaag gaaaacgccc atcatgccca cttccaaaag
    27721 caggaagaag accatgaaat ccttgacccg ttcttcaacc gctgtccacg tggagagcag
    27781 ggaaatgggg gtcaggaagg cggtgagcaa caccagcaaa atgctcaagc cgtccacagc
    27841 cagatagtag tagatgttcc agcccgctac ttgaatccac gggtatttgg cttccaactg
    27901 cagatcgggg ttggacgagt cgaactggga gaaaacccac aacgaaacga caaaggtgat
    27961 caatgatgtg cccaacgcaa cccagcgaat ggcattcttc gactcggaat tcataaagag
    28021 aatcacgagc acgcccacaa gcggaaggaa ggtcaggagt gtaagaggtt gcaaaagaaa
    28081 ttccatgttc tatcctcggt ttctacgagt tatttcaaaa tcaaatagcc taagatcaaa
    28141 accacgccca gcaaaacaga gagcgcatac gaacgcacaa agccattttg cactttacgt
    28201 aacgttgcgg aaacggattg tgtggcagtg cccaaccagt tcgcgaaacc gtcaatgcct
    28261 ttttgatcgg cgtagtgatt cagtgcggtt tcgctgagcc agttataggt cccggcgatg
    28321 accgtgtcgt ggacaaagtc atgccagaag cgccagtcaa tcacatccgc aaggaactgt
    28381 gagagtttgt tgaatggatt gatgatgacc gcgaagtagc cttcatccac aaaccatttc
    28441 ccttccatgc ctgtgaagat aaaaccaagc ggcttcttga gcgggtcgat ctggccagcc
    28501 ttaagaggat tacggccata aatgaaccag gagatcaaaa tggccagcaa agccagtgcc
    28561 gtggaaatgc ctgcgacttt caggtcgagc gggagtcctt caacctcgcc aatggtgtgg
    28621 cccagccagt gaccgagatt gtggaaccct tcaaacggaa gattcaacgc gccgcctgcg
    28681 accgataaaa ctgccagcac cataaggggc acggtcatca ctttggggct ttcctctgca
    28741 tgtttggctg catcggtgcg cgcttcacca aagaagacca tccaaatctg gcggcccatg
    28801 taaaaagcgg tgaagaaggc ggcaatggtc aactgccaat agacactggt aaagtgcagg
    28861 cttgcatcca aaaggatttc gtccttcgac caaaaaccag caaacgggaa gatacccgcc
    28921 aaggccagtg taccgatcat gtacagccag aatgtgacag gcatggtctt gcgaagtccg
    28981 cccatgttac gcatatcacc cgggtcgaag acttcaccgt gctcgtcatg accgccatgc
    29041 ccgtgttctt cctctttctt cccttcggct ttgccagagg cgtgctttcc ttctttcttc
    29101 tttttgccgt gatcatcgtg agcggcatga tgagcgtggg catgatgtcc gcgctccaac
    29161 ccaagaataa cggaacctgc agaaaggaag agcagggcct tgaagaaagc atgggtgatg
    29221 aggtggaaca tccctgccac atacgcgccc atgcctacgg cagccaccat gaaaccaagt
    29281 tgcgaaatgg tggaatacgc gagaactttt ttaatatcgt attgaccgac tgcaatcgtc
    29341 gcggcgaaga gcgcggtccc tgcgccgacc atcgcaacga tatactgcgc ctgcgggaca
    29401 agagtgtaaa gtggagcgga acgcgtcaca agatacacac cagcggtcac cattgtggcg
    29461 gcatggatca acgccgaaac aggggttgga cccgccatcg catctggcag ccagatgtag
    29521 agcggaattt gtgcagattt acctgcaaca cccaccagca tgaacagggt aatcgctacg
    29581 atcacccaag gcattgagtg tgcaatttca ggggcggcct tgaaagcctc atcgaattga
    29641 aaagagccaa gataccaaaa catcaggaag ccagcgatga gaaaaccaaa atcgccgacg
    29701 cggttggtaa tgaaggcctt cttggcagca ttggaattgg cccaggaagg acgtgcaagt
    29761 gtgtccattt cgaaccagaa gccgatcagc aggaaggaac aaagacccac accttcccaa
    29821 ccaacgaaaa gcatcatgta cgagtcgcca gagacgagga tcatcatcgc cgcgatgaac
    29881 aggttgaggt aaaggaagaa gcggttgaac cttccctctt catgtttgaa gcggacatcc
    29941 tcgtgcatgt atccaatcgc atagatgtgg atgagggtcc caacgcccga aaccaccagc
    30001 atcatcgtgg tggagagcga gtccatgcgg aaggtccaat caaggtggag tgtgccgacg
    30061 tggatccatt ctgccagttt ccagcgcacc acctccccgt ggttgaggga aacagaatat
    30121 gccagcagaa cggaaacgac aaacgccgta cccgatgcca ggcttgccac ggtaccaacc
    30181 atcttttcgc tgaattgtct tccgaaaatc aggttgatga gcaggccaat gacgggtgca
    30241 aagaccagcc acggggccag aaagaaaaat cctgtgctaa ctgattcgct taagtgcatg
    30301 gtttctccgt aattggtaat tggagattag taatgactaa ttaccaatta ctttttactt
    30361 ttatcccttc aaggaattca actcatcaat attgatattc ttgcgtgcct tgaagatttc
    30421 cacgatcaag gcgaggccga ccgccacttc agcagcggct actgcaatga caaagaaaac
    30481 gaagatctgc ccgctgaaga aaggctggcc accggcgatc tcgtaggaac ggccaaacgc
    30541 cacgaaggcg aggttgcccg cattgagcat caattcaacg gacatgaaaa tgatcaacgg
    30601 gttgcggcgg atgagtacgc caagcgcacc gatggtaaat aaaacggcgg aaagcgccac
    30661 gtaataattg acagggacca ttatttttgc tctctctttt cctgcttggt caaaacgatc
    30721 gcgcccacca tcgcgaccaa aagcaaaatg gatgtaattt caaaaggcag gagatactga
    30781 ttgaagaggg acattgccat ttcacgcagg ttttccatgg agttgagggc ggcatccggt
    30841 gttgagatga atttatcggt ctgcgcacgg gaaatgacca gatacaccga ttccacgacc
    30901 agcacaacag ccagggtgta agccagcggc ttttgccagg gaagcgactg ggagggcgca
    30961 agattctccg caccaagcag catgatcacg aacaggaaca acaccatgat cgcacccgca
    31021 tacaccgtga tctgcgacat tgcaatgaaa ggggcgccca gcaaaaggta gaacaccgcc
    31081 acagtaacaa aattcaacac caggtacagt gcagaataca ctgcattgcg gctgaagagc
    31141 atccccaacg cgcttgaaat ggcgatcagg gaaaggacga ggaaaacgat tatgtcagaa
    31201 ttcataaaac tccatgtacg gtttgcaatt ttggatttac gagattacgg ttcgtaaatc
    31261 gtccatcgta aaccgttaat cggatggatt ttgcatctca ggcacggagc gggtgaacac
    31321 accgggttcc actttgcgcg gcgtgatcat atccacagtg cggacgggtt cgagcagcat
    31381 ttccttcggg tagatggcct gtcggcggtc ggtgaaagaa agctcgtaat tatcgccaag
    31441 cacaatggcc tcggtggggc aggcatcttc gcaaaagccg cagtagatgc agcgcaacat
    31501 attgatctca taggtggatg cataccgctg tcccggcgag aagcgctttt catctgtatt
    31561 ctcagccgct tccacaaaga tcgcatccgc agggcaggct gcggcgcaca gcgaacatcc
    31621 gatgcacttt tcgaggccgt tatcgtaacg cttcagcacg tggcggccgc gatagcgcgg
    31681 gcggacaggg cgcttttctt caggatattg ataggttacg gtcttctcaa aaaggaagga
    31741 acgcagggtt tcacccaaac cttttgaaag ctcgataatg ggatcaaata cgcccatgag
    31801 attttctcct caagtcactg ttgataaaaa caaccgccac cagcacacaa acgatcgaaa
    31861 gcacaggaat accccaaatg atcatcgggt tgttcaattc ctgagccagc aaaatacctg
    31921 tggcagtgat aaaggcaagg atcagggata acggtaaaag caccttccaa ccgaaagaca
    31981 tcaaacggtc ataacgaata cggggccagg tggcacggat ccagatcata aagaacaaca
    32041 ggacaatgac cttcaacagc aggtagatcg ggccaaggaa ccagaaacgg tccacactaa
    32101 aaatggtccc ctgcaaaaac cagaattcgc gatagccgcc aaagaacaag gtggccgcga
    32161 tcatgcagat gacgatcatc ttctggtatt cagccatgaa gaacaaagcg aatttcatac
    32221 cggagtattc tgcatgataa ccagcggtca attcctgttc ggcttcgggc atatcaaagg
    32281 gagcacggtt cacttccgcc agggttacga tcatgaagac cagcgcacct gcaggttgaa
    32341 tgaccgcaaa ccacatccct ttttgggcgt tcacaatatc gttcaaattc atcgaatcgc
    32401 cgagcaaaat ggcgatcaca atgcacaggc ccagcgaaag ttcataagag atcatctgtg
    32461 ccgaggagcg cacaccgccc aacatggcgt acttgttatt gcttgaccat cccgccagca
    32521 caatgccata caccgcaatg gatgcaatgg aagtcaggta caacacgccc acattcagat
    32581 ctgccacata cagggtgatc tcacggccaa acaatacgaa cttcgagccc aatgggatca
    32641 ccgccgccag gatcaaagag ggcaccacgg tcaacacagg cgcaagcaca aacacaacct
    32701 tgtacacacg tgatggggtg aattcttcct tgaagatcag tttcaccgca tcggcaattg
    32761 gctgcaacac accttgaggt ccagcacgat tcgggcccac acgcacctgc atacgcgcca
    32821 gcgcgcggcg ttcatacaag gtcaaatacg caaagcccgt caaaaggatc aggcagagga
    32881 taaatccctt taatacccat tcaagccaga tggtccaatc catatcactt caccttccct
    32941 gcaagctgaa cacgcgccac aacaggttcg cggatcgcaa tccccatact gcgcggcacc
    33001 agtgcaacgc ctgccgagat cgtatcgtcc aatttcacaa ccacttccgc attcactccg
    33061 tcgaaactga gcttcacacg tgtacccgct tccacgccca aattctttgc agccattggg
    33121 tgcagggtca cggttggctc accaacccac tgaccaagca gcggcgccga tgaactgatg
    33181 gtcacgccat agtcatacaa cctggtgaca ggcacagcca gcaattcatt ctcttttgga
    33241 cgaagagccg cttctttcct gaccttgggt atgcgtacgg ttccaccggc ttgagcggtc
    33301 gggacgagct gcacacccaa cccctgtgta ttctcgtaag ttgtgccgcc gtagtacagg
    33361 tcgccacgcc caacgatcgg ccattgtccg tgcgtttcgg ccagtttggc ataactcaaa
    33421 tcggcaaaag actcaacaga atgtgaaaga atgtcaaaca ccacagaagc cgatgaccct
    33481 tcgaggatca cgcccatttg tttggcgatc tgtgatgtga tggcaaaatc aggttttgca
    33541 tcacccttca ctggcacagc cgcataaaaa cgctgcacac ggcgctcgcc ggaagtgaaa
    33601 gtaccttcgc gttcggtgta ggcctgtgcc gggaaaacca catcagcgat ctcggtggtg
    33661 gctgtttcga gaatatcctg cacggctacg aacttcgcac ccttcaaagc cttggccaac
    33721 gcagggtcat caccaacagg gtctgcccca acgatgtaaa ctgcctttcc tttgagagcc
    33781 ttctccaggt caggttcggg gcggaaacca atttcccagg caccctgatc gttcacacgt
    33841 tcccatacag cgaccagtcc attatttggt ttaccaacat gttcggtgtc tttcaaaagc
    33901 gtggcacagg cagaagcaag gctggccgtg tcattcaaac caagtccatc gttgccgtag
    33961 aaaacaacgg cattctttgc cttggcaaat tcatcagcga tcttgccttt gttgcttaag
    34021 ttagccactg cctttgattc atcaccctgt gcataacgga tgacaaactt ggcaaattta
    34081 tccagtttgg tttcgcgtgc attggcgaca atgagggttg ctccacggtc agcggcttgt
    34141 tttacgcgca gccaccaaat gggggcttcc tgatacaggt ctgaagccac tacaagaatg
    34201 gcatcaccct tgcccatctt accgatgttc gttccctgcc caacaccaac aagtgtggtc
    34261 aaatctccgc cgcccatgtt ggaatacaaa atggcagtac caccagccgt atcagccagc
    34321 gacttcaggt tgaacagatc ttcattggcc aaacggcccg atgccaacac cacgaaatcc
    34381 tttttcgatt tcgtaaagtt ctcggccgcc attttagttg cagcatccca tgaggcacgt
    34441 gccagctttt cttccttgcg cacgagcggg cgggtgaggc gtttcttgct ttcggtaaag
    34501 tgataggtaa agcggccctt atcgcacagc cagatttcat ttacttcttc attctgtcgg
    34561 ggcatcacac gcttcacgac cacatcaccg ccagccttgg cctcacggcg tgtgttgagc
    34621 gtggtattgc agcccaccgg gcattgcgta caaatggaag cctgtgcttt caactcccac
    34681 gggcgcgcgc caaaacgaaa atcggcagtg gtcaatgcac cgacagggca aatgtcagtg
    34741 gtgttgccag agaagatgga atcgaacccc ggttctgaga gtgaaatgat ctgcgtgtta
    34801 cgtccgcgct catcgaaacc gagtactgct tcaccggcca cttcatcctg aaaacggata
    34861 cagcgtgcgc actggatgca gcgttcgcgg tcaagccaga tcaagtcgcc aagcggaacc
    34921 tgcttctcga aatgcgcttt ctcatcataa ataaagcggc tcttgcccgg accgaaagcc
    34981 atggtcaggt tttgcaatgg gcattcgcca cccttgtcac agatcgggca atccaatggg
    35041 tgcgaagtaa gcaaaaactc cactgtacct ttttgcccat ctttggcctt gtcggttgtg
    35101 gtcatcacca ccatgccgtc tgaaacgcga ttggtgcaag ccgtttcaag tttgggcatg
    35161 aactggatct tgggctggcc gttttccatc accacctgac cagtggcacg atccaacatg
    35221 ggacggccca cctccaccag acacatgcgg cacataccgg ctggctccag cttcgggtgg
    35281 tgacaaaata ccgggatgtc gatcccagcc atcttggccg catcagcgat caacgttcca
    35341 tcgggaacgg ttactgttat tccattaatt gatagcgtga cttgtttcgt catgcgtgct
    35401 ccattaagcc tttaccgctt ttttgaaatc ctgcgggaat ctttcgatac cggtcaccac
    35461 cgccatggtg gagaattcac caagtgcgca caggcatttg ccttgcattt gcttggccac
    35521 atttagaagc aggtccacat cgttctgcac tccctgaccc gagtgaatgc gctcggtgag
    35581 atgctgcatc caatagtttc cttcgcggca ggttgtacac ttaccgcacg actcatgctt
    35641 gaagaaatgg atcgtcttgt tgaccaccca atcgatatcc acagtatcgt ccaatacaat
    35701 gacagaagca gaacccagat ccgcgcccaa tgtgcgtacg gtggcgtaat ccatcggggt
    35761 atcgagtact ttatcatcca ccacaatcag tgaagaagat gcacccgcag gcatgatcgc
    35821 cttcacaggt cggccatcca aaacaccgcc gccgtgttcg tagatcaact ggcggaaggt
    35881 cgtgccgaag ggcagttcat aattaccggg tttgcgcaca cgtccggaga gcgagaagat
    35941 cttcacgccc gcgctatcag cggtgcccat agacttgtac cactccgcgc cgttcgccat
    36001 gataaggggg acgttcgtaa aagtctccac attattgatg atggtgggct tgccgtacaa
    36061 accaaaggaa ggcgggaacg gcggacgcac acggggctgt ccgcgtttgc cttcaagcga
    36121 ttcaagcagg gcggtttctt caccgcaaat gtatgcaccc gctcccaagt gagtgtagat
    36181 cttcagggag tattcagtgc cgaacaggtt ctcaccaagg aaacccgcct tttccatatc
    36241 tgcgatcttt tcatcaagga aagcagccac ctgccagaat tcaccgcgca ggtaaatgta
    36301 agctacattg gcgccaaccg cataggatgc gatcgccaac ccttccaaaa actggaatgg
    36361 attgctttcc atgatctcgc ggtctttaaa tgtgccaggc tcagactcat cggcattggc
    36421 aaccacataa tgcggccaat tcttggtatc aatgaaagac cacttcatac cggtggggaa
    36481 acccgcacca ccgcggccgc gcagacccga gttcttaacc acatcggtca cttcattcgg
    36541 ctgcattttg gaaacagcct tcttgaacgc gtcaaaaccg ccgttctttt tatagacttc
    36601 gatcttgttt agatcgacaa tatcgcgatg acgtaaaaga acgttagcca ttatttcacc
    36661 cccccatcag atttcttcag ggcttcgatc aattcaatgg tcttatccac ggtcatgttc
    36721 tcgtgatatt taattccatc agggccctgg gtttggaaca tgggtgcttt gtcgcaggca
    36781 gcaagacatg tcacagcttc gagggttacc aatccatcgg gtgtggtttc gccgactttg
    36841 attccgaggt tgctgcatag atttttcatg aaatcatcgg caccccgtaa agcacagggc
    36901 aggtcagtac aaatttgcat acggtatttg cctgcttttt cgtcgtgata cagcgaataa
    36961 aagccgacga tcgaggcaac ttccgtctcg gtgatgtcga gcatgcgggc aatatcctgc
    37021 atcgcagctt tgttcacata accttcctcg cgctgggcaa ggtagaggag cggcatgacc
    37081 gccgagcgtt tgtgctccgg cggatacttg gacaatattt gcttaacttc cttcggatag
    37141 gatttctcaa ggctcatctg tcaatatctc cgaggacaat atcaatggaa gcaagaattg
    37201 caacaaggtc agccacaagg tgacctttca caatctgcgg aacaacggca agattatcga
    37261 aagaaggtgt acgcaggtgc atgcgcaatg gcttcggacc accatcccct tcaataaaga
    37321 gacccaactc acctcgtgga gattccacgg cgttgtagat cgaagcttgt ggcgccgaga
    37381 aaccttccgt ccacaacttg aagtgatgga tcagagcctc catgctgaca ccaatttcag
    37441 agcgcggcgg cgggacaaac tttcggtttt cagaacgaac aggaccaagc ggcaatttat
    37501 tcaaagcctg ttcaacgatc ttcaaagact cgtgcagttc ttcaatgcgg acaaggtagc
    37561 gggcgtatgt atcaccctcg gtacgggtgg gcacgttgaa atcatattgt tcataaccgc
    37621 agtacggacg caccttgcgc aaatcccaat ccacacccga tgcccgtagt gtaggaccag
    37681 ttaccccgta ggaaagagcc gtctctttac tcaggtagcc gatatccacc atacggtcaa
    37741 taaaaagcgg attcttggtc aataacgatt cgtactcggc aattcgtttc ggaaagatct
    37801 tgataaagtc gcgcacggcc ttttcaaatt ccacgggcac gtcacgccac acgccgccgg
    37861 ggcggaagta ggtggtcatc atacgttgac cggagacaag ctcgaaaata tcaaggatct
    37921 gctcgcgttc gcggaaacaa tacaggaaca tcgacatcgc ggcaaggtca aggcctgaag
    37981 tacccagcca gaccaaatgt gaagcgatgc gctgcaactc aacaaggatc acacgcaacg
    38041 cctgcgcacg cacggggaca tcaaggtcca ccaatttttc cacggccata caatacgcca
    38101 ggttgtttcc catcgtattc atatagtcaa ggcggtcggt catcacctcg gccttttgat
    38161 aggtcttggc ttccatgttc ttctcaacac ccgtgtgcaa aaagccgata tcgggaatgc
    38221 agttgacgac aatttcgcca tccagttcga gcaaaagccg caaaacgccg tgggtggaag
    38281 ggtgctgcgg acccatgttc aaaagcatgg tctcgccggt aagcgcccgc tcagatacaa
    38341 gatgcttgaa tttttccagg ctgacttctt caatctgact cataactatt cctttacata
    38401 cggcttgcgc agatcgatct catcgaagtt gaaggtgaat tgcggctctt catacccaag
    38461 cggaaaatcc ttgcggagcg ggtgccccac atgatcttcg ggcatcaaga tacggcgcgg
    38521 atcgggatga ccttcaaatt cgattccgaa catatcgaag atctcacgct cacgccagtt
    38581 cgccgacgca tatacaccgg tcactgtcgg gaccttggga ctgctcccat ttacagcgac
    38641 tctgacctgc accgaaagat ttttgacaag cgaagtaagt tgatagatca aatggaaacg
    38701 cggcgtttcg ctggggtaat aatccaccgc ggtataagct gacagcaatt caaaaccctg
    38761 ctcgtcgcgt agaagggtca acgcctccac gatctgctcg ggcttcacga acaaatgcac
    38821 ttcatcgcga aactcttcac agcctgcgcc gaatttgtct tgaattgttt ttacaacggg
    38881 ttcgagattt ttattcataa aaaaatattc cttaagacct gacagattgg gcctgtcagg
    38941 tcttatgaat gattattttg cggccagatc cttgaatttt tcacctttga ccttttcata
    39001 cacggtcaac acaccatgta tcaaggcttc cgggcgcggc gggcaacccg ccacatacac
    39061 atccacaggc acaacttcat ccacaccctg atagatggca tagttattga agacaccgcc
    39121 gcaggaggcg caatcgccca tggcgatcac ccatttgggc tcaggcatct ggtcatacaa
    39181 acgacgcagg acgggtccca ttttcttcga gacgcgtccc gccacgatca ttaaatccga
    39241 ctggcgcggg ctggctcgca tgagttccat gccaaaacgg ctcatgtcat aatctgaagc
    39301 ctgtgccgcc atcatctcga tggcacaaca agccagacca aacaacatcg gccacatggc
    39361 attcgtgcgc gaccaattga ccacattctc cagcgtggtg gtaaccacgc ccatatttcc
    39421 aagtttctgt tctactccca ttcaagggct cctttcttcc atgcgtacac ataaccgatc
    39481 aataagataa cgataaacac gatcatctct agcaggccaa agatgcccag cttgcgaaat
    39541 gcgatcgccc agggtagaaa aaacacaact tcaatatcaa aaaggatgaa caacactgca
    39601 atcaggtaaa aacggaccgg catacgccgc gtaccttcac catatggatt catacctgat
    39661 tcataaggca tgtctttggc ggcagtcttt ttcttcgggc caaacaattc ccccaaacct
    39721 atcgcgatga aagcaatcaa tgtggcaacc gcaatcatca ccccaatggc aatatactcc
    39781 agcaacataa acacctcttt ggtgtggaaa atcagtgcgt gaaatctggc acgttcggcg
    39841 tagtatatca taagagactt tcacgagcca gacaaagtgt cacttttcac aaaaatgatg
    39901 ataattgtca taatgggaat agacaagcaa tataaataaa aacagaagga tgaaaacgat
    39961 tgttttcatc cttcatgctt caaaattcgt tctttcccct aatctcccag gtaatctcgc
    40021 agttccctgc tcctggcggg gtgacgtaac ttccgcagcg ccttcgcttc gatctggcgg
    40081 acgcgttcac gggtgacatc aaaatagcgg ctgacctctt caagggtatg ttcccttcca
    40141 tcgcgcaggc caaaccgaag ttcgagcaca tcacgttcac ggtctgacaa cgactcgagg
    40201 gcgtgctgca cctgctcgcg gagcatttcg cgggcagcgg catccatcgg cgacggggtg
    40261 tcttcgtcct caatgaaatc gcccaatgaa ctggaatctt catcccccac cgggccttcg
    40321 agggaaacgg gttcttcggc ggaacggagt accctgtcaa ttttttgtgt ggcttgttca
    40381 agttgttttt gcaggtcagg tttgatcggc ctgccttcgg catgtgcccg aagaatggat
    40441 tgcacatcgg aagcagagag aaaacccgct tccatggcca attcctcgct gctggggtcg
    40501 cggcccaaaa tctgcgtaag gtggcgctgc gctcgcaaaa tgcgcgaaat cgactcgaac
    40561 aagtgcacgg ggatgcggat cgttcgggcc tgttcagcga tggagcggtt gatcgattga
    40621 cggatccacc aggtggcata ggtgctgaat ttgaatccac ggcggggatc aaacttgctg
    40681 accgcacgca gcaggcccat attgccttcc tgaatcagat caagcaaaga catgccgcgg
    40741 ccaagataac gcttggcaac gctcaccacc agacgcaaat ttgcacggat gagcgcctga
    40801 tccccttcca cagcacgggc gtggatcgcg tccatttcgg cgagcaactc atcatcgggg
    40861 ggcaggttgc ggtagagcgt gcgttgcccg ggcagcgcat tctgggcgcg gacatttgtc
    40921 agcagccact ctgcatattt aaacgggagc aggtacaggt taagaaagac accgtaggca
    40981 tgtcgcgcga aattatccca aaggcggtcc acaccccaat ggccgttatc cagataagcg
    41041 cggaaataag aaggtgaatc gaattgccag cccgagtgca gcgattgtga ctcggccacc
    41101 atcagggcca ggttggggag ttcacaatcg agttgttcgg tatcttcgat caggcggtcc
    41161 cacgaagtgg agagttccac cagggcagtg tgataaatgg atgaggccag cgaaacgcct
    41221 ttgcgttgtt ttagttttcg cagcgcagta atatggcggt cggcttcgct aagtgtagcg
    41281 agacgaaact cgctatctga atcaagcagt ttgaccaggc cgatctcgcg caggtacaaa
    41341 cgcacggggt cttcagaaag ttccgattcc agttcatggg aacgcagggg cagaatttct
    41401 tcaagctcat ctggctgaac gagcaaggtc tcatcaggct cgatgacctc atcggctgcg
    41461 gggtcaaaca acagattgtt tccgttggaa agcgaatcca gcacatcgct ttccgggaat
    41521 ttcatgcttt tttctttcgc tctacgcccc tctggttttt cagcgttttt tatttttggt
    41581 tttgctactt tcccgtgctt ggttttggat ttggatttta cgggcatcgg tttcttctcc
    41641 cagatggagt ctctatcgtt ttgaggatag ctttcttttt gcctgatcca aactgtgaag
    41701 caatcttgtc atttgcaaag tttgctcctg atacagcttc atatttgccc cgccctgctg
    41761 ttggtcttcc tcctgaagaa aacgcaattg attgacattc aacatcgcat ttgcacggcg
    41821 caagtccatg aagcgcgcca gcaggtcatc gaggatcttg tcttccacag ggtcaagagc
    41881 atcggaacgg gcgaccaatt ccttcgaaag ctcactgacc gattccggca cgtgggccat
    41941 cacataatga tgataatcct tctcatcctg ttcaactgcc atgcggacaa caccgaacaa
    42001 caactgatgg tcagtatact cgaaatcttc cacagccaat gcagccaggt cggcttcctg
    42061 catgcgttta tcgagacggt acagcaactc aggcttgcgc agcaaaacac tgaggcaata
    42121 ggattcgatc ttcaaagtgg gattgaccgc cacaatgcca gacgacacaa ccttgtgact
    42181 ttgctccaca ctccgcggct tgcgcctcgc cactggaccc tgcacctgcg tgcccgttaa
    42241 tacgctttca tttacccgca acattcgcgc cagcgcctga cgataggtat cgcgctccat
    42301 ggcgctgggc aggtcttcga tcagcggaag cacctgtgcg gcgatctggt tcttcacttt
    42361 ggcatcgttc aggttctgac cagccgccag cgaatccatc acatgggtga cgatgggctt
    42421 ggcattctcg atgagcttct tccattcttc ctgatcgcgg gctacgatct catcggggtc
    42481 aaggtcatcg ggcatggtcg ccacacgcag gtctgcttgc aggcgggcct catttttcag
    42541 caggccgcgt gcatcgaagc caagttcgcc ttcgcgatcc atcgcagagc gggcggcatc
    42601 aagtccgcgc agaacggctt tctggcctgc cgcatcaggg tcaagcgcca gcacaatgcg
    42661 gcgcgtggac ctcttcaaca ggcgcagttg atcttcagtg agcgcggtgc ccattggcga
    42721 aacgacattt tcatagcctg cttgatgcag ggcaatgaca tcgagataac cttccacgat
    42781 cacagcctga tctgccgcac gaatgggctt gcgggcgcga tcgaggccgt aaagcaaacg
    42841 gcccttggaa aaaatgggtg tttcgggtga attcaaaaat ttggggacgt cgttcggatc
    42901 cacgatgcgc gcgccgaaac ctgccatctt tccattctcg tcgcggatgg gaatcatgat
    42961 gcggttgcgg aatttatcgt aggtgcgggc ttcgtcattt tcacggacag tgaccatgcc
    43021 tgagtcgatc agatcctgct ggctgtagcc tttggagagg aagtgtttca acaggttgtc
    43081 gtatccgggc ggggcataac ccaggccaaa ggtttcgatg gtggagtcgg tcagtccgcg
    43141 cttttcatgc aggtaggcca gaattgcagg ggcgccaaaa agctgggtgc ggtaataaac
    43201 gaccgcatct tccagcagtt tgcggagatg gtcgtaggct tctttttgtt ggggtgtttc
    43261 gcgctgatac tctttgatct ccacgcctgc gcgcagagcc agtttctgca tggcctcttt
    43321 gaaatccagg ccgtcgcgct tcatcacgaa cttgaaaata tcgccgcctt cattacattg
    43381 gccaaagcag cgccacgtgc cagattcagg ccagaccaca aaggcgggtg tgtgtttgtt
    43441 atcgtggaaa gggcaaaagc ccgtgtagtt cttccccgca tgacgcagtt ttaccccggc
    43501 ctcggaaacg aggtcaacga tatcaatacg ggctttgatc tcatcaatgg tggacatggt
    43561 tctcctttga attatacttg ccccctatga attcaaggct tatcaaaaac aaggtgtttg
    43621 cgcttccatt tttggcggtc tatgcgtggc tggtgatccg cacttcgtgg gtgggggacg
    43681 atgcgatcat caccttccgc tcgctggaaa atttcctgca tgggtatgga ccggtattca
    43741 acatcggcga acgggtgcag acgtttacgc atccgttgtg gtttttgttg caggcggcgg
    43801 cgggggctgt gttcaacctt tggaagagca atccctttgg tcaggggcag atgtattttc
    43861 tgaatgtctt catttccatc ggcatctccc tgctggccgc ggccatcttt acttttaatg
    43921 aaacgtcctc tgcaaaaggg gcggctttgg ggcttttgat cttttcggtc tccaaatcat
    43981 ttgtcgatta ttcgacctcg ggcctggaaa acccgttgac ccatttgatt ctgctgctat
    44041 tcaccttgac ctatgttggg cctgccaaaa actcacagcg ccgactctac ctgctggcaa
    44101 cgctggcagc tctcggcggg gtggatcgtc ttgatacgtt gttgttctat ttcccggcgc
    44161 tggtcctgct gttttgggag agtccgcaca aagggaagac cttcctcacg cttgggtttg
    44221 ggtttctgcc cttgatcgcc tgggaattat tttcgctgtt ctattatggt gtgccgttcc
    44281 caaatacggc gtatgcaaag atcaacacgg gcattcccga ggcgagtctt ttcatgcagg
    44341 ggctgttcta ttatttgaat tcgttgaagc tcgaccccat cacattgctg accatcttta
    44401 cggtttcggc ggcggggtgg atgagcaaaa acaaaaagca tcaggccatc ctggctgggg
    44461 tactgctgta tctgctttac acgatctaca tcggcggcga tttcatgagt ggacgttact
    44521 tctccagtcc gttgctggtt tgcgtgaccc tgctggcaac catggatttc gaatcgccga
    44581 aaaaattcgg ggttgcagtc ggaatcgttc tgctggtggg gctggctccg atcgcagtgg
    44641 ttgcagaacg tgcgcctgcg tttggcgtgg gtgatgaggg cgatcaccgc gtatttgtgg
    44701 atgaacacgg aatctcagat gaacgcatcg tgtacaccgg gttggggctg tttgaacgct
    44761 tgaagaaaaa aagcgctcct tccaccaatt atgcgcgtga caagtgggtg tacctgcccg
    44821 atgaacctgt gcgggtgcgc attgtgggtc cgctgggttt gaactcaatg acgcttggtc
    44881 cgaactttca tgtcattgac ctgaactcac tggccgatcc attgatgacc cgcatgccgc
    44941 tttacgagat cgaccgctgg cgcatcggcc attttcggca catcattcca aagggatatg
    45001 aagaaacgct caagagcgga gagaatcgca ttgtgaacaa ggatattgcc cagtactacg
    45061 acaagctctc cttcattgtc aggggcgagc tatggagctg ggaacgggtg gttgaaattt
    45121 ggaatttcaa cacgggcaag tatgattatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45241 nnnnnnnnng ggcaagtatg attatttgtt ggaaaatgtg acgcccgccg acgcgatgga
    45301 ataaaaaaaa gctgttgacg atgaaggact gttctttgaa ttcactgtaa agatttacgg
    45361 ccatcccatt tctgacagga tggccgtaag ttcaaaaggg tttttagtgc gaacacgttg
    45421 acaggtcagt acaggttcgc gaatcgtatt ttcgggcgac atgaatgtcg ccttacgggg
    45481 aaaccgtggg gattagattc aacagcctgc gcacgttcat gttcaacatg gaataatcca
    45541 tcatgccgac ctgcccgaac gagatattgc cgttgacatc cacaaaatag ttgacgggga
    45601 acaagttggt gccgccatat ttcttgaagg tactggcttt cgagtcgatc aacatgggat
    45661 aggtcaggtt gtgattatct cgataggtgc gggcatggtc ggaatctccc cacacatcca
    45721 ctgcgaggac gaccagtccg atatctttat attgagtgta gatatcctgc atggctgcgg
    45781 cttcagtgtc acaatagccg cagccgttgg cccaaaagaa gatgacaaca ggtttgcctt
    45841 tgtaatcgct caaactgacc tgattgcccg tattgacatt ctttagcgtg aaatcgggag
    45901 ccgccatccc cgtgccgagg ccgacttgtg tctctgcatt ggggtcttcg gtgccgatga
    45961 cattggaaat gggtgtggac gtggactcaa tataacccag tgtgctggtg ggaataagcg
    46021 tggggctggg agcgggagtc cacgtggggg gcaaaccgat ggttggaatg gtggtcggtg
    46081 tttccgtggg gccaaggaag atcggctgtg cagtgggggt ggagatctgc tgtccacgca
    46141 ggacgaagaa gaagccgacc aaaatcaggc caaacacgca cagaaaaacg acgatcaaaa
    46201 cgatgtattt aacaaaatcg ttcttctttt tcggggcggg ctttttgcgc gcaggttgtg
    46261 cgggtttgtc cgcagactgt gtttcggttt gaaatggaga aacgcttgat gagtacggat
    46321 tttcataggc atcctgtccg aaggaatgtt caaaagcatc ggcggaagga cgcgggaggt
    46381 cttcatcctc gggtaaaaac aggggtggaa tggaggaaga ttgatatgga tcctggctgg
    46441 gggtattcga gtaggaacgc tcgaacgcgc tcaaaggcgg cgctgattgc aggtcaaggt
    46501 catcttcggg gaagaacggt gagggtttgt aattatcctg cgcggcctga taggaagagt
    46561 tggaaatatt gaaggcatca cggtcatcga tactgtcatc gggcaaaaac gtggctgcct
    46621 caggcgggcg gacattccct tgactctggc gaaggaagga ggcctcgaaa gcatctgaag
    46681 aagtctgcgg ggccgattca tcttccaaga tgaagggagc agtgatcggg tgaagacgag
    46741 acggttcctg actgaatgtc gaatctgatt tcggggcgag gtcgctttgg agtttcaata
    46801 atcggttgcg ggcggggata ttccccgggt cgagtttcaa gacccgttcc acacaatcca
    46861 attgttgttt ggggtcggtc aaaatgaagc taagcatcca ccaggcacgg gaagaatcgg
    46921 gatggctttg gacatactgc accagcgcag tccgcccttc ctgttttttc ccgtcacgca
    46981 ggagctgttc gatgtattcc aagccgttgt tttggtcagc catgatgaaa ttatagactc
    47041 cgcttcagac aacgcaaaga tgacatttat cctaagatgc aagtacggcg ggaccaaatc
    47101 atcccgccgt actgataagc tattggtaca tggcgcagta taacgcctgc agacggtcat
    47161 acccctatga aattcattgc aaaagccttt tgtttagaag ccttccaact tgctcagatc
    47221 agcaatgccg ttcgaaagcc cggcgatctt ttgcagcaac ccaaggcggt tttccttgac
    47281 tgccttatct tcgaccatga ccagcacttt atcgaagaaa gcggtgatgg cagggatgag
    47341 ggccgagaca ttgttcagga aggaatcaac atctcctgca accacaggcg gcagacggtt
    47401 gaccgcttca tacaaccctt tttctgcttc ttcaacaagg gatttttcac tcaccttgaa
    47461 agtttccttc tggtcacggg taatgcggac acaacgggcg aaaccatcca aaatggatga
    47521 ccaatcttca cgttcgaccc aagccgacag ttgatccact gcgcggacag ttcccgcagg
    47581 gttattggat tgcgccgcaa gaaccgcctc aatgacatcg tgcttgaatc ccatatcacc
    47641 aagggccacc ttcatacggc ccgtgatgaa ttcgaggatc tgtttttgta cttcatcact
    47701 gaccgggatc ggctgggttt gcgcagagag tttgaccgct ttcgcaagat cgaaatccat
    47761 cggagacatc ccgaccgctg gcgacatcag cggcatgacc acgccaatgg cagcgcggcg
    47821 cagaccaaag ggatcctttg cgcctgtggg ggcaagcccc gcaccgaaca taccgaccaa
    47881 cgaatcgagg cggtcggcca gtgcgatggt caacccggcc tttgtctgcg gaacactttg
    47941 atactgctcg ctgatcgcag ccgcaacttc cttctgttcg ccggagcgaa gggcatattc
    48001 accgccgata atgccttgca gggaggtcat ttcggtgacc atctgtgtga cgagatcggc
    48061 tttcatgaga tgtgcagcgc ggcgggcgaa gatagcctgg tcggcttcga ggccgagaga
    48121 gggaatcaac tcattgacca gtttcacgat acgctcagat ttatccaaca tggagccgag
    48181 cttggtgtgg aaggtgagtg atgacagttt gggacgatac tcttcgagct tgagtttgac
    48241 atcttcacgg acaaagaagt tcgcatcggc aaagcgcgcg ccgaggacgt gttcgttgcc
    48301 ctggcggacg gtgtcaatgt gaatgtcgtc gccgtttctg atcgcgatga attttgccag
    48361 taggggcgac ctgccaaggt ctgaccgagt atccgattgc gattgaaggg tcgcccctac
    48421 gcgttggaca gggaagtacc gctggtgctt tttcattacc gagatcaaaa catctctcgg
    48481 caaggcgagg aactcttcat tgaatccgcc catgacggcg gtgggcattt cgatcaggtt
    48541 gaccacttcg ttgagcaagc cttcttctat aatggcttgt ccaccgacga g
//
LOCUS       DAOQJC010000008        47668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_307_length_47668_cov_9.125722, whole genome shotgun sequence.
ACCESSION   DAOQJC010000008 DAOQJC010000000
VERSION     DAOQJC010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 47668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47668
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_307_length_47668_cov_9.125722"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PLE39_02050"
     CDS             complement(<1..560)
                     /locus_tag="PLE39_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67131.1"
                     /translation="MKSLKIKLPVGQVFFQAVLWLLVFCAAVEAFARTPTGRDFFQYE
                     SYGSSHPHFDPQVIRIKAREVQDGQIDCIILGDSQVLYGIDPTIVEQAYFDSTGNTIH
                     CQNFGLGGLKPITAYPLAKLLIKNFHPAIIVFGTDLFDYTAVQAGSDSSIMSSPWVKY
                     QLDDFSVDGWLIENSNSYRFYLGADR"
     gene            complement(609..1376)
                     /locus_tag="PLE39_02055"
     CDS             complement(609..1376)
                     /locus_tag="PLE39_02055"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNE67132.1"
                     /translation="MDYSSAELMIINAARLLRDGDVVFVGVGQPNLACNLAKRTHAPN
                     LVMVYEAGVIGAEPARLPLSIGDPTLVSGSVSVVSMYDIFANYLQRGNVDVGFMGGAQ
                     IDKHGNINATVIGGYDHPKVRLPGSGGSQEIAAWANRCYIMTPHQKRRFPEKVEFMTS
                     AGFIGGKGDREKAGLRGKGMVGVVTDIGMLEPDENGEMVLTALHPGKTVEDAKANTGW
                     DLKVAAEVRVTEPVTENELRILREELDPTGIYLKSGG"
     assembly_gap    1393..1492
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1563..2558
                     /locus_tag="PLE39_02060"
     CDS             1563..2558
                     /locus_tag="PLE39_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67133.1"
                     /translation="MKTKNPTAIFSIILILSVLACNLPGSATPQATPVPILPTETPAA
                     SASATPTTVPTETEIPSPTVVPPTPTIAVFHSLVPATNIRAGDLIHDVVSVDTAAENR
                     APYGDSYNINLFERPFTQDMTYIPDVDIVSYNLSHDEKFYYVSIELVGKNPNNEIGIN
                     YAVELDLDADGFGDYIIAARPPYNAEWSTDGVVVAKDSNRDTGGLSAEKSDAPYPGDG
                     YNEIIFDSGLGSVNDSDLAWVRINAGRYATVQFAFKLWVAENKFMYGVLADAGPRSIA
                     DLDYVDRYTEEEAGSPVRSDKFYPLKALFGVDNVCREVQGFTGTGQEPQRCLPKK"
     gene            complement(2680..4011)
                     /gene="ffh"
                     /locus_tag="PLE39_02065"
     CDS             complement(2680..4011)
                     /gene="ffh"
                     /locus_tag="PLE39_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNE67134.1"
                     /translation="MFETLTGRLNQIFDNLRRRGKLSEADVDAAMKEVRLALIEADVH
                     FSVVKTFIARVRERAVGAEVSKALNPGQQVIKIVNEELIASLGEPVGLNLTGPKPRVI
                     MMVGLQGAGKTTASGKLARLMKAKGERVLLVAGDPYRPAAVQQLQQLGERLDVEVEAD
                     LSLTPPQLAKRAVDKAEKGGFSIVIVDTAGRSQMDAALMDELKAIAGNINPVDILLVV
                     DSMIGQEALNIAQGFKDAINLTGLILTKMDGDSRGGAAISIRSVTGVPIKFIGTGEKL
                     DALETYDPSRLSSRILGMGDLIGLIEKAEAAYDAKDMDAQARQAEKMMKGHFSLEDWL
                     DQMKQMKKMGPLAQIMEMLPGQMGQAARGIDPSLVEKSFKQSEAIINSMTLKERRNPD
                     ILNASRRRRIAAGAGMEVQDVNRLIKQFREAQKMMKMFQKTGGKGLGKLFG"
     gene            4175..5533
                     /locus_tag="PLE39_02070"
     CDS             4175..5533
                     /locus_tag="PLE39_02070"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE67135.1"
                     /translation="MAKEGAMVNDRREIFGWAMYDWANSAFSTTIGTVFLGPYVAGLA
                     RSAAEAAGTTNVSFLGIPVAPDSFLPYCISFSVGMQVLFLPILGAIADYSHLRKRMMQ
                     VFATIGAIATILMFLVTGGLWWLGGLLFIVANLAFGAAIVFYNAYLPDIASPDETDRV
                     SSYGWAMGYMGGGILLALNLAFFTFAEDLGVPSGLAVRINLASAGIWWLGFSLITWSR
                     LRPRHAARQLPAGDTYVSIGFKQLRKTFSEVKHFPETLKFLLAYFLYNDGIQTVIAVA
                     STFAAAPLIQGGLELEQSTLIAVILMIQFVAFFGALFWGKLAGWIGAKQSVVVSLVIW
                     AGVVIYAYGGLKGESATAQFFVLGVFIAIVMGGSQAISRSLFAQMIPNGKEAEFFSFY
                     EISERGTSWIGPLVFGLANQIFGNLRYAILSLIFFFIMGLILLPFVNVSKAVEDAKKY
                     EG"
     gene            complement(5592..6689)
                     /locus_tag="PLE39_02075"
     CDS             complement(5592..6689)
                     /locus_tag="PLE39_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67136.1"
                     /translation="MGIFIKVFVFIAGLVVVALTVFSALSTFVLPRAARSQLNRLVFG
                     VMRRVFEFILRFFPTYEQRDSIMAYYSILGVMMLVPVWYFLVAAGYAAMYWSLGVGDL
                     YADFRFSGSSVFTLGFATSDNPIVTFLSFSEALIGLVLVALLMAYLPTMYAAFSRREQ
                     AVNMLEVRAGNPPNPYDMLSRFHRIHGLDKLGDLWKNWEIWFADLEESHTTLPALVFF
                     RSPRADNSWVTSAGAVLDTAALTLSSIDIPNEMSAALCIRAGFIAFRRIADYFDITHP
                     QNPTYPETPISVSRAEYDEVIRKLEAAGVPIKPDREKAWRDFAGWRVNYDRVLLVLCT
                     LVMAPQVPWSSDRAPKFKMPPLFVPKKKLKH"
     gene            complement(6690..7322)
                     /locus_tag="PLE39_02080"
     CDS             complement(6690..7322)
                     /locus_tag="PLE39_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67137.1"
                     /translation="MRIGKSSLFLVFALVVLTLPTACNLGVVPPEASPPPVAIVETPT
                     FVIPPPVEPTATPTLIPTIVPTATVTLFHSSIPTLTPTSQWTGCPGIVVKVDDTKKGD
                     ILNILRCDDGLEYQLGPLAKGVYAVGPNNNFLIYVSFDGIVYAARIGDRYLNPLYNLG
                     REHIFTVFNKKVAPDFQLSFTGEGPIYRLVILEKNYDQKRMYELPIGLTE"
     gene            complement(7431..7655)
                     /locus_tag="PLE39_02085"
     CDS             complement(7431..7655)
                     /locus_tag="PLE39_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67138.1"
                     /translation="MAPMVHGLEAEYFGKVKFSYLDADDPRTDGFQRALGFYYQPELY
                     LLDANGNVLKKFIGLTSREDLVAEFEKYLQ"
     gene            complement(7671..8000)
                     /locus_tag="PLE39_02090"
     CDS             complement(7671..8000)
                     /locus_tag="PLE39_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67139.1"
                     /translation="MRPHFPDRIQRHGSFLTVVLVAVFAASACAPAAATNPPPAAELP
                     TATTAPAAEAIPTATGEVAAPVPTQAVAEVLPVATSRGPNLEATDPTTVNLAAGQLQL
                     VEFFRFT"
     gene            8225..9799
                     /locus_tag="PLE39_02095"
     CDS             8225..9799
                     /locus_tag="PLE39_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE67140.1"
                     /translation="MADFKLTYATMFNPPEELHTGFDKAVEKVKKNMGQEYTMLINGK
                     EVKADDKFDDFSPVNTDWKLAVMQKGNAKHAQEALAAARAAFPMWSHTPWQKRVELLR
                     KAASLIEERIFDLGAAMALEVGKNRMESLGDVQETADLIYYSCYQMEKNDGYIVEMGK
                     DPLVGYDARNVSVLRPYGVWLIVSPFNFPFALTGGPAGAALVTGNTIVIKPATDTAWI
                     VRLLVDCFRDAGIPDGVVNFVTGPGSTLGQALVDSSEVDGVTFTGSFDVGMKMFQDFG
                     KRNYVRPIILELGGKNPAIVSKNADLERATIGIIRSAFGLQGQKCSAASRVLVEDSVY
                     DDLVGRLKEAASKLVLGDPTDRNTYNGPVVNKSSYNDFKNFCEEINQGGGKFLVGGNV
                     KTGGMFDKGYYCESTFVVDLPFSHRLWKHEMFLPITTIGRVKNLDEAMSIANDVNYGL
                     TAGFYGSTEETEWFFDKIEAGVTYANRPQGATTGAWPGFQPFGGWKGSGSSGKNAGGH
                     YYLPLYMHEQIRTLVK"
     gene            9870..10622
                     /locus_tag="PLE39_02100"
     CDS             9870..10622
                     /locus_tag="PLE39_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE67141.1"
                     /translation="MPWDPTQYHKFQAERSAPFFDLLKLVNIRPNLQAVDLGCGTGEL
                     TKHLTEALPEGTVLGLDSSLEMLNKARQHEGPSLRFEQGDQSELQGDWDLIFSNAALQ
                     WSENHEALIPRLFGRLKENGQLAVQVPSNHFHISHQIIRETAREEPFQTALGGFNRQA
                     PVLSIERYAEILFECGAKDITVFEKVYPHVLENSDAIVEWIRGTALVPYLERLGQQKE
                     AFTETVRTKLKSAVPGSPVFYPFKRTFLSAVK"
     gene            10647..10910
                     /locus_tag="PLE39_02105"
     CDS             10647..10910
                     /locus_tag="PLE39_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67142.1"
                     /translation="MDDDFVFRIGMFFYVIGGGSFVLFVTSDIADKVDFDFLFVALLM
                     FGIGAYLRRGMAPPPSAGRFASFKKWRENAKNKKQQKQEAKKK"
     gene            10912..11814
                     /locus_tag="PLE39_02110"
     CDS             10912..11814
                     /locus_tag="PLE39_02110"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNE67143.1"
                     /translation="MTKLITLKQAISDFVKDGDVVYAAGFTHLIPFAAGHEIIRQGRK
                     NLTLARATPDLIYDQMVAAGCAKKVIFSYMGNPGVGSLRIVRAALEKGELEWEEYSHF
                     GMITRLQAGAAGLPFLPMNQTGATDLERSNPLIKRIPDPYGGKDVIVVPALNPDVAIV
                     HVQRADKNGNAHLWGIIGEQKEAAFAAKKVILTAEEIVEESVIRSDPNRTMIPANIVD
                     AVCHVPFASHPSYSQGYYDRDNEFYLAWDKISESQDSVKAYLDEWVFGVKDREEYWQK
                     LGSEKREQLKVVSALSEPVNYGKY"
     gene            complement(11920..12396)
                     /locus_tag="PLE39_02115"
     CDS             complement(11920..12396)
                     /locus_tag="PLE39_02115"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HNE67144.1"
                     /translation="MTVTNNVTRFLDARKVKYEAHELPPEKLGAVEAAQHMGVPPEQV
                     FKTIVVTREKKKPLLTVIPGPRVVDLKKLAAFLNEKKVSLPTEREAEALTGLQAGGIS
                     PLALINKGFQVVIDSAAQGFEKIYISGGQRGLDIQLPVADLASLTNAKFGDVSRDE"
     gene            12531..12983
                     /locus_tag="PLE39_02120"
     CDS             12531..12983
                     /locus_tag="PLE39_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE67145.1"
                     /translation="MTTASVLSQFGLFQDLPQQLLEEIAAISDEVEMKQGEFVFREGE
                     KADKLHFLLNGSVALRVKLTSRPESLTVSFVSTPYQSFGWSGIVAPNHYTSSAECDED
                     SKLLITPAQPFLKLLEKYPEAGFKVMQRIAEIIADRLRNSRQALIKTL"
     gene            13083..15314
                     /locus_tag="PLE39_02125"
     CDS             13083..15314
                     /locus_tag="PLE39_02125"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNE67146.1"
                     /translation="MKSSNTAIPLEKLIEQLPEQYTVADRELVQRAYRVAEEAHREQK
                     RNSGEPYINHCIAVASILSDLKVPPEVIAAGLLHDTVEDTTITLADIRRDFGDTIKIL
                     VDGVTKLTHLPRVSRGDQHPEKDENGNHTEEKPIEPALLGRKEDIVSETLRKTFLAMG
                     DDVRVILIKLADRLHNMRTLGHMPEHKRRRIAQETLDIFAPLANRLGIWQIKWELEDL
                     GFRYVNPEKYKEIAELLTEKRPDREAQLEAIKENLVKLLEKNNIKAEVSGRPKHIYSI
                     FKKMQKKGKPFDMVRDVRAVRLIVPDMPSCYAALGVIHTTWRPIPGEFDDYIAAPKDN
                     FYQSLHTAVIYDDKRPLEVQIRTNEMHLNAEYGIAAHWRYKEGTKHADRNYEQRINSL
                     RTMMEWRSDVKDAAEFVESMRSDVFQDRVYIFTPRGDIIDLPAGSTPIDFAYHVHTDI
                     GHCCRGARVNGKLVPLYQELKTGDQVEILTAKRGAPSRDWLNPNLGLVRTQRARSKIK
                     VWFKKQEDEQNLAQGRDALEKELQRLGLQDINFERMARDLEYKTPDEMFIALGNGDLS
                     VSKVIKQFSQQEAASDIFEVNPSTTPSTSTNAIEVVGLKGMLSTMARCCNPMPGDQVV
                     GFITRGRGATIHRQDCPNILILQTKERERLLQVGWGHVERTYPVPIKIKAYDRQGLVS
                     DISNLLADENINIADVKVNVNRSMADLRLIIEVKDLTQLSRVLTRIENLPNVMEAHRV
                     NSG"
     gene            15336..16652
                     /locus_tag="PLE39_02130"
     CDS             15336..16652
                     /locus_tag="PLE39_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67147.1"
                     /translation="MKSNFWEKSADILRRNAALFAFLLALGLYGLSAGIKLTQQSKTP
                     QYVYLAYSFLQGHTDLVELPESRYDLIEFKGKWYVPGGITPALLSLPFVILFGKGFSD
                     VLFGVLVGATNAALMAVLLGQIVEDFSTRIWLIALFIAGTVHWWLASVGSVWFNAQIV
                     ALLFMLLYARDAIRNRPWLAGLWLGLAFLARPPTIFSALFFLLLVVLRERSITPVLKK
                     MIPFGVMLAASVAVMLTYNQLRFGNPVDFGYGYVRGSNALIQTFARSGGFNVQYMPCN
                     IHVSIFGLPNLNTGIFPSVNEACSYLEPISHDFGKLSKFFNPLGMSIFLATPAFLLIF
                     RAKRRDDWVIPAWAGMIGILIPLWMYHATGWVQFGYRYTTDFMVFLFILLASAIKEIR
                     WMEKSLIGMSVFMGGTGMYLMYYMTFGLVWPEMFMELARKIYHFIF"
     gene            16654..18411
                     /locus_tag="PLE39_02135"
     CDS             16654..18411
                     /locus_tag="PLE39_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67148.1"
                     /translation="MKRQKFNPVTMTILLLVFASVAFRVQAIPNSNRDMAVYNLRWYQ
                     EISQKGIGNALGTNFSNYAPPYTYLLALATLTRDLIPPLTAIKLIPIFFDLLGAYFIY
                     RIVRLKYPQGNLSMLAAAIYFSAPTVVLNSAYWGQADSIYTSALLACLYFLLTEKPLP
                     AMLAFGAAFSVKAQAVFLLPFLGVMVLRKKIHWWVVGVIPLVYLLAIAPVVLLGRPLM
                     DALLVYAKQSDTFNMLSMNAPNIYSLFQREWYMVVLPFGLVLTAALTLGWMYSSWQAK
                     TDLDDKHMVLLAFVSAMLIPFLLPKMHDRYFYPADVLSIVLAFYWPALWFMPILLQIS
                     SGGAIAVFLFGADPAFTVYGFLLNSIALTIAMHTQRVSEKRQTHPLVSSSLAWLAAFL
                     VPLALFGIGARLLLTPTFIRAEYALPYTQEAQGLSRSERFQQASQTLEYMGSDRKPQF
                     LQRLRLDNGAPVFTEQEITTLESVKQSLRTTFSIWSLSLAVLFLLTLLAQAGEWLSKL
                     RHGVQRGGWITVAVAVISAAAVIFVPIKVFEGEMLSRLFPNTLWRDAVLILCLFMAAS
                     GFLLAKSLSTLGGNTASSQ"
     gene            18454..19677
                     /locus_tag="PLE39_02140"
     CDS             18454..19677
                     /locus_tag="PLE39_02140"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNE67149.1"
                     /translation="MYSVIEARERILSHFESTPHESVPLVACSGRVLAVDVTAAHDLP
                     PFDNSSMDGFAIRSADSANAAVQGLTLSVVADIPAGSAPKVTLATGQAARIMTGAQLP
                     AGADAVIPVEDTDFHVRDAGTTPPETVSFSRKVTAGENIRPRGMDLHAGDVVLKKGRK
                     LKPQDLGLLAMLGIAKVDVHKKPRVALLSSGDELLEVDSPLSEGKIHDSNSYALAAAF
                     ESVGAEVIRLGVAKDTRESVEALLKKSTLEHADLIVSSAGVSVGAFDFVKDVIETNGK
                     LDFWRVNMRPGKPLAFGSYKGLPFIGLPGNPVSAFVGFEVFVRPALEKLSGLLDGTRL
                     TVKVRCGEVIESDGRESYLRANIRLENGIQTAFLTGHQGSGNLLSLVQADALLIIPAG
                     VKCVPVGQEVEALLL"
     gene            19684..20112
                     /locus_tag="PLE39_02145"
     CDS             19684..20112
                     /locus_tag="PLE39_02145"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNE67150.1"
                     /translation="MDKWLYRASVALVVLGLLVSIYMTIYKYSGNDNMCLGSGDCHTV
                     NASRYSEVNGIPVAVFGIGGYLAILAVLYFENRNRFFRENATLMVFGMALTGFLFTVW
                     LIYVEIALLKAICPFCVTSQVSMTLIFILAVWRLIRQPQS"
     gene            20124..20357
                     /locus_tag="PLE39_02150"
     CDS             20124..20357
                     /locus_tag="PLE39_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67151.1"
                     /translation="MPRIKCHYIDCVFVDEGYCSAAAVEFDPDTGCNTYSAADGVAPK
                     DDDWDEEDETEEWDDDAEEEEDDDWLDDEEDEF"
     gene            20552..21034
                     /locus_tag="PLE39_02155"
     CDS             20552..21034
                     /locus_tag="PLE39_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67152.1"
                     /translation="MSRKTIIYSISVIMICTGLVQIWRFFSPLFGAGKSMNLTLILSG
                     AFVLSAGWNLLKLNTTGRELAFWILFMNLLGGLLVLGFILPVNGIVSINVQFLDKPVF
                     NSVDERLFSIVFLAVFLVIDLSALVFLEQEKTKKLFAPDLVENLTSKADAESKSTSEK
                     "
     gene            21107..21484
                     /locus_tag="PLE39_02160"
     CDS             21107..21484
                     /locus_tag="PLE39_02160"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNE67153.1"
                     /translation="METTKSKFASIDEYIATFPKETQKLLNDLRTAIKASAPNAEEKF
                     SYGIPTFFLNGNLVHFAAFKTHIGFYPAPSGIEAFKKELSMYKQAKGSVQFPLDAPLP
                     LKLVGKIVKFRVAENLKKTSKKK"
     gene            complement(21512..22282)
                     /locus_tag="PLE39_02165"
     CDS             complement(21512..22282)
                     /locus_tag="PLE39_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNE67154.1"
                     /translation="MEMITSLANPLVKHARALRQKKTRLETGLFLVEGIHHVGEAIEA
                     DWKIETVLYAPDLLTSSFARNLLTRLKTPPQPVSVSVMESLADKENPQGIIALVRSRQ
                     TQLTDLKKFSSAVALVSPQDPGNVGTILRTMDAVGAEILFLLEGGVELYHPTVVRASM
                     GTLFWKPIVQASFDDFIAWARKGGYQLIGTSAKADVDHHTLTPHTPWILMLGNEQKGL
                     TTEQSAACDVTVSLPMKGRVSSLNLAVAAGVLLYQFGE"
     gene            complement(22351..22749)
                     /locus_tag="PLE39_02170"
     CDS             complement(22351..22749)
                     /locus_tag="PLE39_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNE67155.1"
                     /translation="MKKIVVVFLVLVFLLTACGGNKPSKNLRVDMLEFTFTPAEFVVP
                     AGEEITITVANNGAVLHEFVIMKFGQTVGDDFGDEDEGNIYWEVEVEPGKTVTTTFTA
                     PADSGEYQVVCGTAGHFMAGMAGTLVVAAP"
     gene            22887..23549
                     /locus_tag="PLE39_02175"
     CDS             22887..23549
                     /locus_tag="PLE39_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNE67156.1"
                     /translation="MNTLVQKSNTAVYWQYALWLAIFTVFYNIAEGLVSISFGVSDEA
                     LTLFGFGVDSFIEVISGAGILAMVLRIRQNPNTSRTRFEVTALRITGTSFYLLAVGLG
                     VSAIYNLVIGHKPDTTLPGIVISVISIAVMWALVMGKRKTGRMLNSMPILADANCTMV
                     CIYMSVILLISSLVYAVSGFGFIDSIGALGLIYFSYKEGREAFEKANGLECTCEDEHC
                     KD"
     gene            23576..24793
                     /locus_tag="PLE39_02180"
     CDS             23576..24793
                     /locus_tag="PLE39_02180"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HNE67157.1"
                     /translation="MKFLRWLALAATMLLSACQSNPSPSITIIDSGQIQVVASDSRIP
                     LVLLTQNGITPQQFDRVLVNGVPFPIDQPLPSVDSVQLQLRRAVDLTLTTPQGTETFQ
                     TSALNVGQALAEVGYQIGAADVLDPPAETPITTAMNVTFTPARDLTVQVGESVVHIRS
                     AAGTVGEALAKAGIPLIGLDTSFPLSGEALPADGQIQVVRVQEIIDVAIEPIPFETKK
                     ISSIDVPLGQEELVQSGVNGIAMTRTRIRYENGQEVSRILEEEIVAREPQPRIVKSGE
                     QIVMSPLPGVSGEYWTAVEMYATVYSPCESGTGGCSYSTASGARAGYGIVAVDYSIYS
                     YLAGMRVYIPGYGTATIGDTGGGPIIETAFGVPRTKWIDLGFDDGAIQDMTGWVTVYF
                     LAPAPAEVPYFLK"
     gene            24905..26032
                     /locus_tag="PLE39_02185"
     CDS             24905..26032
                     /locus_tag="PLE39_02185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNE67158.1"
                     /translation="MTQSQKRILAILSFVAFVLVVTAGFFAFRSYRAFVTQPLGPSLP
                     IVAQTMPPTWTPAATTSPNMITLAPTLSFTTPTPYAGCGGTTSVMHVVAIGADSRSDN
                     YQYGLGDVIRLVRIDFVTPKVSVLEFPRDLWVEIPYISDNLNGQDHEKLNQAYLYGQP
                     GDGFHYWDDPSAGPGLLSLTINLNFGAQVDHYVAVNMRTFVNVVNAVEGIDINIPDET
                     AAENTGLTIGKHHLNGDQALKVARNREEGVFERASNQNRVLCALRQKLASPAVVTKIP
                     ELIDSFQDNILTDFTPEQIAQLACLGTKLPPENIVFASFPQELFKESRIYDPVFKKNV
                     FYWDVDFNILRDYTAKFQAGTWPEPNTITTDEEDEEEIICE"
     gene            26029..26859
                     /gene="rsmA"
                     /locus_tag="PLE39_02190"
     CDS             26029..26859
                     /gene="rsmA"
                     /locus_tag="PLE39_02190"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNE67159.1"
                     /translation="MTDYRSIPPLDAHAVLKRFQLRADKSLGQNFLQDSSALEKIARA
                     AEIQGDDCVLEIGPGLGSLTRYLAASAQKVTAVELDPDLLAPLRAVLSPYQNVNVIHG
                     DILKLNISEIIEQPDYLVAANIPYNITSAIIRHLLESTPKPRRVVLTIQKEVAERICA
                     APGDLSLLALSVQVYGRPSIAAKIPAGAFHPVPKVDSAILRIDIHKEPLIPAELLNTF
                     FKLIKAGFSQKRKTLRNSLSSGLHIPPHEAESMLTAAGIDFMRRAETLSIEEWRRLCG
                     "
     gene            complement(26856..27125)
                     /locus_tag="PLE39_02195"
     CDS             complement(26856..27125)
                     /locus_tag="PLE39_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67160.1"
                     /translation="MDKKIKPSEVIAARFQVSNETAKFFLGRVQKSFKTERPPHQLVV
                     DFMSGQSYDALPKPYQIAKALNEKGIWAYPLNSEPAEPVDEDELY"
     gene            complement(27179..28009)
                     /locus_tag="PLE39_02200"
     CDS             complement(27179..28009)
                     /locus_tag="PLE39_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE67161.1"
                     /translation="MHARINHIHFVEDSQKSPVVFVHGFPDSPAMFAAYHTEAERRQA
                     WLQGRSIYAYAFPNRHDNPNFPSLGELAGGVMDREFNALMDELARNSPTGKLVIVAHD
                     WGATHTWRWAREKKHPPIEKLIALSVGSSFRYDVFEHGLNAFTWLYGLWFCAAWYLPF
                     LRKTVAASIVSRAGYHSETAHELWKDAYHYWDRPSLLITIFLQALFFLFYKPEYLDFD
                     FPILYMRTKLDRIASTARFEQALRSRPDARFVLFEQFNHWFPEQHSDLVLAEIRSFVD
                     "
     gene            complement(28099..29430)
                     /gene="ahcY"
                     /locus_tag="PLE39_02205"
     CDS             complement(28099..29430)
                     /gene="ahcY"
                     /locus_tag="PLE39_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNE67162.1"
                     /translation="MSNYDIKDIQQAEGGRRRIEWAEREMPVLRSIRERFAKEKPLKG
                     MRLSCCLHVTTETANLMRTLHEGGADIVLTASNPLSTQDDVAAALVNQYEIPVFAIKG
                     EDKVTYFKHIRAALEHKPHMTQDDGADLVSSLFFIEMGRFERLEPSLAEWAKTLSDEE
                     RKQMLKNVIGGTEETTTGVIRLKAMEKDGVLKFPVIAVNDALTKHMFDNRYGTGQSTI
                     DGIIRATNVLLAGKTFVVAGYGWCGRGLASRARGMGAHVIVTEIDPMKALEAVMDGFQ
                     VMPMFDAAKVGDIFCTVTGDLNVIDGKHFEVMKDGALVANSGHFNVEINIPALEKMSV
                     GAPNLVRPFVDQYLTKDGRKINILGEGRLINLASAEGHPASVMDMSFANQALSAEYMA
                     KNADKLEKKVYSVPTEIDNEIARLKLEAMGVKIDILSDEQLHYLNSWEEGT"
     gene            complement(29500..30456)
                     /locus_tag="PLE39_02210"
     CDS             complement(29500..30456)
                     /locus_tag="PLE39_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNE67163.1"
                     /translation="MDILLTGSVAYDYLMTFPGLFKEQILPERLASISLSFLVESMSK
                     QRGGIAPNIAYTMTLLGEKPRVMATVGEDFGDYRAWLDSKGVDTELMKVVPGLFTASF
                     FATTDQASAQIASFYPGAMGHSATQSIKELPKKPDLVTVSPSAPDAMMKFPAECRELG
                     IPYLYDPSQQVLRLEGHELARDMEGAHFLFCNDYEFGLISKKTGWSLDQILGHVKVLV
                     ITRGKDGADLYAGGESVHIPTVPEDEIVDPTGVGDAFRGGFLAGYANGFDWKLCGEIG
                     SLSAVYCLEQRGTQNHSYTKQEFIQRFRDHFDDSGRLDALLK"
     gene            complement(30593..31372)
                     /locus_tag="PLE39_02215"
     CDS             complement(30593..31372)
                     /locus_tag="PLE39_02215"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HNE67164.1"
                     /translation="MKRVFSMLAGFCLLVLLGGCGPSAEQISTMTASAWTPTSPPTNT
                     PVPTNTPLPTETPAPTAKPPFLFRDDFDGQLADGWVWLGEDTTHWSLAEVPGFMRIIT
                     QGTNIGSDAEPINFLVHAAPAGDFEIETYVQFEPTTNFQFAGLLIYEGQGQALGFGRA
                     FAQCNFPPCKGNALYFDNPTQQNGPNFATPITSPSEVYLRLLREGNTYTAYYSEDASN
                     WTQIGQHTSDITPAFVGLIASQAYEEEKPADFDYFSIRAIP"
     gene            complement(31627..32385)
                     /locus_tag="PLE39_02220"
     CDS             complement(31627..32385)
                     /locus_tag="PLE39_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67165.1"
                     /translation="MLFPYSPPTRFDLRFSIAGIPIRVQPLFWLIAVLLGSTSGGIIS
                     LVIWVLVVFISIIVHELGHAFALRRHGISSNILLHASGGLTVPESMRWGGGIASVALT
                     PNQEIFVSLAGPFAGFGLALLVSVLTFIMGGSIVMGTILFVIPVPVHASLPFNLYLLD
                     LFLTDLLWVNIFWGVFNLVPVYPLDGGQVTRYILLIADPWDGLRKSLWLSFIAGIVIA
                     AIAFFLLGSTYMAVLFGFLAFQSYQALQGGLNKL"
     gene            complement(32386..33426)
                     /gene="mtnA"
                     /locus_tag="PLE39_02225"
     CDS             complement(32386..33426)
                     /gene="mtnA"
                     /locus_tag="PLE39_02225"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNE67166.1"
                     /translation="MRTVFWENNELKMIDQRILPARFEILSYRGHKEVAHAITDMVVR
                     GAPAIGAAAAFGLALAGYESASTSTEGLLADLRASAAVLKASRPTAVNLAWAVDRLMR
                     VAEQMKDEGNADAVRAAVLTEAQNLADEDVEINKRMAEHGAALINDGDTIIHHCNTGA
                     LATVDWGTALGVIRTAHEQGKRIHVLVDETRPRLQGARLTAWELEQYGIPYEIISDNM
                     SGYFLKAGKAQKVFFGADRVAANGDVANKIGTYMLSLAANDNNVPAYAVVPTSTVDLS
                     LAHGGLIPIEERNTAEVLGIQFQGEPVAPKNAKARNPAFDVTPNRLLSGIVTENGVVY
                     PPFDMNLKKVVK"
     gene            complement(33455..34120)
                     /locus_tag="PLE39_02230"
     CDS             complement(33455..34120)
                     /locus_tag="PLE39_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNE67167.1"
                     /translation="MLNFSPTASEAELRLAIIECGRIAYERHLVTSNDGNISARMLDG
                     NILITPSGISKGRLNPDDMLVVDLEGKVISSRPDRKPSSETPMHLEVYKQRPDVRGVL
                     HAHPIYATTLTVSGLQFPVDVLPEVLLTLGDVPITAYATPSSHEDAEVIRPFVKTHNA
                     MLLCQHGSLTYGKHLDEALIHLERIEHVSEIYWRAKMLGEVKRVPPEAQAKLIELHEK
                     YFS"
     gene            complement(34130..35275)
                     /locus_tag="PLE39_02235"
     CDS             complement(34130..35275)
                     /locus_tag="PLE39_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNE67168.1"
                     /translation="MNIHHKIYHFGRLILSAFMLLSIAACGARPQESTEHVTSSGFVC
                     PEPSPRVEFESKEINIFTWTEYVPADMIDCFGLVYGVDVNVDYFSSNEELYTKMSFGE
                     SVNPYDVVHPSDYMIDVLIREGLLQKLDAGKLANTKNLDGNLISAYGNSLDFLVPYQM
                     GTQAIVYNSETVKTPPTSWADLWSPEYEGRIVAVDDNRVVIGMALLTLGYDVNSTDEA
                     QLEEAKQKLIELMPNIRTFDSDSPKTPLVAGDVDLGIVWNGEAFLARQENAAFEYAFP
                     KEGSIIFFDGMGIPTNAPHPDISYAWFNYMMQGDVFWLTLVDYPYTIPNQAALDFAKA
                     NHAEVYEAYMASPITNTPAEIFAQGHKVEDLGNALSLYDKIWTEIKE"
     gene            35399..37876
                     /locus_tag="PLE39_02240"
     CDS             35399..37876
                     /locus_tag="PLE39_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE67169.1"
                     /translation="MNFTRPSTRRIDSDTRRSRLRTGLRFFLGLWLATTITACSIFPN
                     FPTLPPGWTLTPSTTPTPEATSTPTITPTPLPIARVDKGDHALFNGDYESAMLHYQTA
                     LSDSPDPLVQAAAKWGEARVRFAEERYEETLLSTQELISQFPESPNLGHAYFLQGLAN
                     YRLGNYQAAADSWQTYLVVRPGHLDAHVQELRGDALFEARDFPSALSAYTAAIQASAL
                     GDDTLLDLKVASTQVAIGDYNSALALYDGVFARASNDYIKAQTAYESGLAYQAIGQNE
                     EALGRFRLAVEQYPLSYYSYLSLVALLEAGGTVSQLDRGLVDYFAGQYDVAVAAFNQY
                     LVENPTNDGTAYYYRALSHRDLGNYDAALQDYKKFIEGYPTHPRWVDAWGEKAFIEWY
                     NLGSYTVGIKTLLDFVAAVPGSETSADYLMSAARIYERNGKYEEAIQIWARVANEYPG
                     SEQASTATFLTGIIHYRNGNTVPALDAFNRSLNLSVLAVDQSRAYLWLGKAQQKLGDG
                     EAAVNAWRAAQDRDPGGYYSERARDLLLERQPFQAPISVNLTPNLESERKDADSWVRL
                     TFNLPLETDLSGLGSLASDPRAIRGTEFMELGMYEDARLEFENLRAELETNMDAVGSY
                     RLMNYLVDIGLYRSAIFAARQVLTIAGLDEHTESMMAPPYFSHVRYGLYYSDLVLPDA
                     DSNRFDPLFVFSVIRQESLFEGFVKSSAGARGLMQVIPSTGAQIVSQLGWPVNYTDDD
                     LYRPDVSIAFGTHYLGNNRDLLGGDLYAALAAYNGGPGNAIEWRDLGQGDPDLFLESV
                     RFEETRNYIRNIYEIYTIYRRLYGLSE"
     assembly_gap    37944..38043
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(38139..38447)
                     /locus_tag="PLE39_02245"
     CDS             complement(38139..38447)
                     /locus_tag="PLE39_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNE67170.1"
                     /translation="MTRKFVLHSGQRGSALAVRITPRASRNQIVGVLSDGTVKVHLAS
                     EPADDVLNVELVGYLSEVLGVPKTRVEVVAGENGRDKLVSILDMDVETAHQRILSHME
                     "
     gene            complement(38456..38722)
                     /locus_tag="PLE39_02250"
     CDS             complement(38456..38722)
                     /locus_tag="PLE39_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNE67171.1"
                     /translation="MDFQILILLIRNLSTLFIWVVIAYSLLSFFVPPYHPVREALERI
                     VSPFLDPIRRILPAAGPMDFSPVVLILAIDVLSRILIAFLISLS"
     gene            complement(38722..39459)
                     /locus_tag="PLE39_02255"
     CDS             complement(38722..39459)
                     /locus_tag="PLE39_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNE67172.1"
                     /translation="MSDLVKSIRERYLATLDKIASAARSAGRNPDSVRLVVVTKAQPV
                     EVVRAVIEAGAQVLGENYPEESVKKIQYLGQFSAVEWHMIGHVQSRKAQLVAANFNYL
                     HSLDSLKLANRLDRFCSEAGRVLPVLLEFNVGGEESKSGWSAWDEAQWPALRDELGVV
                     MSLPNLKVHGLMTMPPLGETAEFSRPFFQKLKRLQAYLSTQLPKSDFSELSMGTSSDY
                     EVAVQEGATLVRVGTAIVGPRQYKTEV"
     gene            complement(39491..40273)
                     /gene="pgeF"
                     /locus_tag="PLE39_02260"
     CDS             complement(39491..40273)
                     /gene="pgeF"
                     /locus_tag="PLE39_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNE67173.1"
                     /translation="MPFQEHDGLRYYRFDIFSNAVTQAVFTRRGGVSPAPWHSLNLGG
                     SVGDDPAHVAANRIRVFQAMGCEPESIHDVWLVHGTDIVYADAPRPLDEPSAKADIIF
                     TDNPRVSLFMRFGDCVPILFHDPKKKVIGIAHAGWMGTLRGVVGAAVDGMRSHYGSDP
                     KDIVAGIGPSIGVDHYEVGAEILAQFREKFNSHADAMFEFREGRIYLDLWKSNSMQLQ
                     QAGVEQIQVSEVCTACHLDDWFSHRAEKGKTGRFGALMALQA"
     gene            complement(40273..41283)
                     /locus_tag="PLE39_02265"
     CDS             complement(40273..41283)
                     /locus_tag="PLE39_02265"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNE67174.1"
                     /translation="MLNVTLYTRKNCKLCDEVKHELQELQSKYPHRLVEVDIESDTAL
                     IDKYGIEIPVVEVGPYTLKAPISRQNLQMTLGAAADRKNQLEQVGDPDYQIRVKKGKT
                     VTTGDRVSFWIAKRYLLLLNLFMLFYVGLPLLAPTLMKLGAELPARVIYRIYSPLCHQ
                     FGFRSFFLYGEQAFYPLAEANVAGVKTFEEVTGIPNLDDPYSFTRFEARAYIGDETVG
                     YKVALCERDVAIYLAILLFGITYGLTGRRIKSLHWVLWLLIGIAPIGFDGFSQLFSQF
                     NWTWLATVVPYRESTPFLRALTGALFGLATAWFAYPNIEESMAETRQYYIKKFAVNEA
                     SQ"
     gene            complement(41388..42113)
                     /locus_tag="PLE39_02270"
     CDS             complement(41388..42113)
                     /locus_tag="PLE39_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNE67175.1"
                     /translation="MEFSQISVGLAFLAGLASFLSPCVFSLVPAYVGYLGGRAAGGES
                     NSKNRWITFSHGLAFVLGFSFVFVFLGVAAAFAGGVLFDLRFWLTKIGGVVVIAFGLH
                     MIGVFHIPFLAYDTRVQQAPDPKMGYLSSALMGVFFSAGWSPCVGPVLGAILTVVFNG
                     GSISQGAVLLTSYSIGLGIPFLFAALGIGWVTNILRNYAKTMRYVEIVMGVILLVVGV
                     MLLTGVFETLALQGQGFYIDFGL"
     gene            complement(42169..43305)
                     /locus_tag="PLE39_02275"
     CDS             complement(42169..43305)
                     /locus_tag="PLE39_02275"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNE67176.1"
                     /translation="MKQNFSRRDFLKVAGVGLGALAFKPYFSETYYEKLYTPKRLPQF
                     PAGDIIGRLVDQTDVRSRPDNNPILNNVIGAMPADYLMPWGREVIGSVIGLTNQKYLE
                     TPQGYVWASRVQPTRNLPNTPITEMPAGQPGFWAEVTVPYVDLTLETAPISPWIQSLI
                     QYNFPPRVYYGQVLWIDQVRTTNGFAEYRWNESPGHGYGFGDIYWLDGAGLKVLTDVD
                     VAPISPEIDPNEKKILADLTYQTLSCYEGNNEVFFCRISSGAKFDATGALSEERATPV
                     GELLTHWKIISLNMSGGGSASGYSTPAVPWSTVIHGDGIAIHGAFWHNDFGERRSHGC
                     INVTPEDAKWIFRWTTPYITLAQCETRMTWPDHGTVVTTKELKL"
     gene            complement(43403..44062)
                     /locus_tag="PLE39_02280"
     CDS             complement(43403..44062)
                     /locus_tag="PLE39_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67177.1"
                     /translation="MPDANNSTLITFNDWTLRVRESNSDSPRLLVMLHGFTGDENSMW
                     VFARRISTDYHLMAPRAPHPTEPSGFSWRPSQGGTFGRPSLEMLQPAAEALIHLIDEY
                     ADSVKLDASQFDLMGFSQGAAMTNVVGLLYPQRVRKMGVLAGFMPSGLDEQIAQKVLA
                     GRNIFVAHGTQDQMIPVDRARASIELLEQAGAQVTYCEDEVGHKLSANCLSALENYLN
                     N"
     gene            complement(44046..44273)
                     /locus_tag="PLE39_02285"
     CDS             complement(44046..44273)
                     /locus_tag="PLE39_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67178.1"
                     /translation="MSRDLKEYEKQTNRRLIIGAMVLLLVVGVGLIWLIYGGGAASMG
                     FFCLMVGLVPVVLIVGIFYAADWIMKNAGRK"
     gene            complement(44270..45151)
                     /locus_tag="PLE39_02290"
     CDS             complement(44270..45151)
                     /locus_tag="PLE39_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE67179.1"
                     /translation="MEITLALGGGGAKGNSHLGVLRRLLKEGFLIRSVAGTSFGGLVA
                     VLYAIGYSPDGIQEIFETVDQSRLYGRHPEDGPSLLGLAGVRKLLDEVVGQRTFKDLR
                     IPCAVTAVDVKSGCEVTISEGPLKDAILATIALPGIFPAQRINDWELVDGGVLNPVPV
                     SVARMLSPKLPVVAVMLNDPMDKPVPAYTIPMPQILPRSIAERITRMHFAQSLDIFLR
                     AVDVSSRAVAHFRLELDKPDVLIRPNVHDINLLDKVVVSDVIKLGERATEEVLPQLKR
                     AVSWPARVNRRIFGAIQ"
     gene            complement(45322..46395)
                     /locus_tag="PLE39_02295"
     CDS             complement(45322..46395)
                     /locus_tag="PLE39_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNE67180.1"
                     /translation="MIVEILNLKKSRSRNLSRWFSFLLFSVLVCAVLLPTPSKAESLP
                     RQTINTPSQLIDAVNNLRIANGLPALAVHSVLMQSSQSQADYMASVGYATHERPGGIS
                     FTQQLLSLGFPLAGDLSLGGFRAENVIQSSNPLVWNGVPPAWQDSQHMNTMLSQNFTH
                     IGAGISQGPSGYFYALDCAAATGSGEMQDGVTTLLTSVPGGAQAGVSQYMVPVMLSTA
                     NANGDVFHKVQYGQTLWSIAIEYGTTIKAIQALNGLGEDLVVYQGQELLVLKGVTPPP
                     ATPTMVQVMETPTSAELITTEMPIPPVTPTSIPQVVTVPATDMPVEEEGSSSNSSGLL
                     VGILVVAAFVGGGVAVWLIRDPN"
     gene            complement(46437..>47668)
                     /locus_tag="PLE39_02300"
     CDS             complement(46437..>47668)
                     /locus_tag="PLE39_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE67181.1"
                     /translation="LWFRLLKNGLFFNVPELIYNLLMENLKKYFYLDPSVTFLNHGSF
                     GATPKPVFEDYQNWQLRLEQQPVLFLGREYDQLLFDARAKLGVYLNADPQDLIYVPNA
                     THGVNVVARSLHLEPGDEILTSDHEYGACDYTWDFVCRKLGAKYIHQPIPLPAYIEDE
                     IVDQLWQGVTPRTKVIYLSHITSPTALRMPVEKISRRAKAAGILTVIDAAHSPGQIHV
                     DLQKVDADFVFGNCHKWMLGAKGSAFLYVRRELQHLIDPLIVSWGYNATPETTTGSRF
                     IDILQWTGTKDPAAALTVPSAIQFMEENNWDEVRKESHDLLRQTLEQICEMVKMESLY
                     PLDSDFYNQMGIAPLPQSNLATLKSRLYDEYKVEVPLIQWQNRQFVRISVQGYNSQED
                     LNKLVKALQILLPQVAV"
BASE COUNT        11748 a        12542 c        12008 g        11170 t
ORIGIN      
        1 taccgatccg cgccgaggta gaagcgatag gaattcgagt tttcgatgag ccagccatcg
       61 acagaaaaat catccaattg atatttcacc cacggcgagg acatgatgct cgaatctgac
      121 ccagcctgta cggcggtgta atcgaacagg tctgtcccaa aaacgatgat ggctggatgg
      181 aaattcttga tgagcaattt tgccaaaggg taggctgtga tcggctttag cccgcccaac
      241 ccaaagtttt gacagtgaat cgtgttcccc gtcgagtcaa aataggcctg ctccacgatc
      301 gtcgggtcaa tgccgtaaag cacctgcgaa tcgccgagga tgatgcaatc gatctgcccg
      361 tcttgaactt cgcgcgcctt gatgcgaatc acctgcgggt caaaatgcgg atgcgaactg
      421 ccgtatgact cgtactggaa aaaatccctg ccagtgggtg ttctcgcaaa agcctcaacc
      481 gccgcacaaa ataccaacag ccagaggaca gcctgaaaga atacctgccc aactggcagt
      541 ttgattttca aagatttcat tttgccgatt atacccgctg gttacgagac aaaatattta
      601 ccacagatct atccgccgct cttcaaataa atccccgtcg ggtcaagttc ttcgcgcagg
      661 attcttaatt cgttttcggt cacaggttca gtgacacgaa cttccgccgc gaccttcaag
      721 tcccagcctg tattggcttt ggcgtcttca acggtcttgc ctggatgcag cgcggtcaaa
      781 accatttcgc cattttcatc gggttccaac atgccaatgt ccgtcaccac gccgaccatg
      841 cccttgccgc gcaagcccgc cttttcgcgg tcgccttttc cgccgatgaa tccagccgag
      901 gtcataaatt ccactttttc agggaagcgg cgtttttgat gcggggtcat gatgtaacag
      961 cgattcgccc aagccgcgat ctcctgcgag ccgcccgaac caggcaaacg cactttcgga
     1021 tggtcatatc cgccgatcac cgtggcattg atatttccat gcttgtcaat ttgcgcgccg
     1081 cccatgaaac ccacatccac gttaccgcgt tggaggtaat ttgcaaaaat atcatacatg
     1141 ctcaccaccg aaaccgaacc gctcacgagg gtcgggtcac cgatggaaag cggcagacgg
     1201 gcgggctccg cgccgatcac acccgcttcg tacaccatca ccagattcgg cgcgtgtgtg
     1261 cgcttggcca aattacaagc caaattcggc tgccccaccc caacaaaaac gacatcgcca
     1321 tcgcgcagca aacgcgcggc gttaatgatc attaattcag cggatgaata gtccatggtt
     1381 cctctatgtt ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaggaact
     1501 attgtgcccg aagattgccc gtctgacaag ggagggataa aatggattac gaggagagac
     1561 atatgaaaac caagaatccc actgccatct tcagcatcat tcttattttg tccgtgctgg
     1621 cttgcaacct gcctggctct gccaccccac aagccacacc cgttcccatt ctgccaacgg
     1681 aaacgcctgc ggcgtccgcc tctgccacac cgacaactgt ccccaccgaa acagaaattc
     1741 cctccccgac agtggtcccc cccacgccga ccattgctgt tttccattcc ctcgtccccg
     1801 ccacgaacat cagagcaggt gaccttatcc acgacgtggt ttccgtggat accgccgccg
     1861 aaaaccgcgc cccctatggt gattcgtaca acatcaacct cttcgaacgc cccttcacac
     1921 aggacatgac ctacatccct gatgtggaca tcgtcagcta caacctgagt cacgacgaaa
     1981 aattttatta cgtctcgatc gaactcgtcg gcaagaatcc gaacaacgag atcggaatca
     2041 actacgcggt tgaattggat ctggatgccg acggtttcgg tgactacatc atcgccgcca
     2101 gaccgcccta caatgcagaa tggtccacag atggtgtggt ggtcgccaaa gattcaaatc
     2161 gcgacacggg cggcctctcc gccgaaaaat cagatgctcc ctaccccggt gatggctaca
     2221 atgaaatcat cttcgacagc ggcctcgggt cggtcaacga ctctgacctg gcgtgggtcc
     2281 gcatcaacgc gggcaggtat gccacggtgc aattcgcctt caaattgtgg gtcgctgaga
     2341 ataaattcat gtacggcgta cttgcagatg caggcccaag aagcatcgcc gacctcgatt
     2401 acgtagaccg ctacacagaa gaagaagcag gctcacccgt ccgcagtgac aagttctatc
     2461 cgctcaaggc cctcttcggc gtggataacg tctgccgcga agttcaaggc ttcaccggca
     2521 caggccagga gccgcaacgg tgcctgccga agaagtagaa tttcgtaccg caacgtttac
     2581 gttgcgtatg atcactatct caaagtcaaa tgtacaaggt gagttaaata aaaaagggac
     2641 acagcggcgc tgtgtcccta cattttccaa tgaaatgatc tacccgaaca atttccccaa
     2701 ccccttgccg cccgtctttt ggaacatctt catcatcttt tgtgcttcac ggaattgctt
     2761 gatgaggcgg ttcacatcct gcacttccat gcctgcgcca gcggcaattc ttcggcggcg
     2821 cgaagcgttc aaaatatcgg gattacggcg ttccttcaaa gtcatcgagt tgatgatggc
     2881 ttccgactgt ttgaaggatt tttccaccaa agacggatca atgccacggg cggcctgccc
     2941 catctgaccg ggcagcatct ccatgatctg tgcaagcgga cccatcttct tcatctgctt
     3001 catctggtcg agccaatcct ccaacgagaa gtgacccttc atcatctttt cagcctgccg
     3061 cgcttgcgca tccatatctt tggcgtcata ggcggcttcg gctttttcaa tgaggccgat
     3121 cagatcgccc atgcccaaaa tgcgcgagga caaacgcgaa gggtcgtagg tttcaagcgc
     3181 atccagtttt tcacctgtac cgatgaactt gatcggcacc cctgttaccg agcggatgga
     3241 gatcgcagcg ccgccgcgtg agtcaccgtc cattttggtg aggatcaagc cggtcaggtt
     3301 gatcgcatcc ttgaagcctt gcgcaatgtt caacgcttcc tggccgatca tggaatcgac
     3361 cacgagcagg atatccacag ggttgatgtt cccggcgatg gccttcaatt catccatcaa
     3421 tgccgcatcc atttgagagc ggccagccgt atccacgatc acgatgctga atccgccttt
     3481 ttcagcttta tctacggcgc gtttcgcaag ctggggaggt gtgagcgaaa gatcagcttc
     3541 cacttccaca tccagccgtt cgccgagctg ctgcaattgc tgaacagccg cagggcggta
     3601 tgggtcacca gccacaagca aaacacgttc gccctttgct ttcattaagc gagcgagttt
     3661 accggaggcg gtcgttttac cagcgccctg caaaccgacc atcatgatga cgcgcggctt
     3721 cgggccagtg agatttaatc cgacgggctc gcccagcgat gcgatcaatt cttcgttgac
     3781 gatcttgatg acctgctggc cagggttcaa cgcctttgaa acttccgcac cgacggcgcg
     3841 ttcgcgcacg cgggcgataa aggttttgac aacgctgaaa tgcacatccg cctcgatgag
     3901 cgcgaggcgc acttctttca tcgcggcgtc cacatccgct tcggagagtt ttccgcggcg
     3961 gcggagattg tcgaagattt ggttgagtcg tccagtgagg gtttcaaaca tgggtaaatg
     4021 ctccagagta ggaacagcat tttagcccgt agcaggttca aggtcaagca ggaggcgggg
     4081 tttcatctgc atatatttct ttacattgaa accgaaactc cttatactcg cctgtgttct
     4141 tgcttgcatc cactttttaa ctcatggagg agttatggca aaagaaggcg caatggtaaa
     4201 tgacaggcgg gagatctttg gctgggccat gtacgactgg gcgaattcag cgttcagcac
     4261 gaccatcggt acggtattcc ttgggccgta tgtggcgggg ctggcgcgct ccgcggcgga
     4321 agcagcaggc acaaccaatg tttcgttttt ggggattccc gtcgcgcctg attcgttcct
     4381 cccgtactgc atttcgtttt ccgtggggat gcaggttttg ttcctgccca tcctcggcgc
     4441 cattgcagat tattcgcatt tgcgaaaacg catgatgcaa gtctttgcga ccatcggtgc
     4501 gattgcaacc attttgatgt tcctcgtaac aggtggattg tggtggctgg gcgggttgct
     4561 ctttattgtt gccaaccttg cctttggcgc ggcgatcgtt ttttacaatg cctatctgcc
     4621 agacattgcc agccccgatg aaaccgaccg tgtttcttcc tatggctggg caatgggtta
     4681 catgggcggc ggcattttgc tggcgctcaa tctggctttc tttacctttg ctgaagacct
     4741 gggtgtacca agcggattgg cggtgcggat caatcttgcg tcggcgggta tctggtggct
     4801 tgggttttcg ctcatcacat ggagcagact gcgcccgcgc catgcggcgc gacaactgcc
     4861 cgcaggcgat acgtatgtca gcattggttt caaacaactt cgaaagactt tcagcgaagt
     4921 gaagcatttc cctgagacct tgaaattcct gctggcatat tttctctaca acgatggaat
     4981 tcagacggtg atcgccgtcg cttcgacatt tgccgccgcg cctctgattc agggcgggct
     5041 ggaacttgaa caatcgactt tgatcgccgt gattttgatg atccagttcg tggcgttctt
     5101 cggtgccttg ttctggggca agctggcagg ttggatcggc gccaagcaat cggtggttgt
     5161 aagtctggtg atctgggctg gagtggtgat ctacgcctac ggcggtttga aaggtgaaag
     5221 tgcaaccgcg caattctttg tccttggcgt tttcattgcg atcgtaatgg gcgggtcaca
     5281 ggctatcagc cgcagtctct ttgcccagat gatccccaac ggcaaggaag cggagttctt
     5341 ctctttctac gagatcagcg aacgcggcac ctcatggatc ggtccgctgg tgttcggtct
     5401 tgccaaccag atctttggga atttgcgcta tgcgatcctg tcattgatct tcttcttcat
     5461 catgggcttg atcctgctgc cgttcgtgaa tgtgagcaaa gcagtggaag atgcaaagaa
     5521 gtatgaagga tagtggaaaa cagtaatcgg taatcagtaa gcggactggc taacgccagt
     5581 ccgcttttgg tttaatgttt gagtttcttt ttcgggacga acaatggcgg cattttgaac
     5641 ttcggcgcac ggtcgctgga ccagggaacc tgcggcgcca tgaccagggt acataaaacc
     5701 aaaagcacac ggtcatagtt gacgcgccag cccgcaaaat ccctccatgc tttttcacgg
     5761 tcgggtttga tgggcacccc cgccgcttcc aatttacgga tgacctcgtc atattctgcg
     5821 cgggaaacac tgatgggggt ttcagggtag gtgggattct gtggatgtgt aatatcgaaa
     5881 taatcggcaa tgcgccgaaa ggcgataaac cccgcgcgga tgcacaacgc agcagacatt
     5941 tcgtttggaa tatcgatcga agacagggtc agggcggcgg tatcgagcac ggcccccgca
     6001 gaggtaaccc acgaattatc cgcgcgcggc gagcggaaaa agaccaaagc aggcagagtg
     6061 gtgtggcttt cctccaaatc ggcaaaccag atttcccagt tcttccacag atcgcccagt
     6121 ttatccaacc cgtgaatgcg gtggaagcgt gaaagcatat cgtagggatt gggcggattc
     6181 cctgcgcgga cctccaacat gttcacagcc tgctcacgcc gtgaaaaagc cgcatacatg
     6241 gtcggcaggt aggccatcaa aagcgccacc agcacaagcc cgatcaacgc ctcggaaaaa
     6301 gacaaaaagg tgacaatggg attatcggaa gttgcaaacc caagcgtgaa gacggaggaa
     6361 ccgctgaaac gaaaatctgc atacagatct cccacgccaa gcgaccaata catggcggcg
     6421 taccccgcag ccacaagaaa ataccagacc ggcaccagca tcatcacccc aagaatggag
     6481 taataagcca tgatactgtc acgctgttcg taggttggaa aaaacctcag gatgaattca
     6541 aagacgcggc gcatgacccc aaacaccagg cgattcaact ggctgcgggc agcgcggggc
     6601 aaaacaaagg tggaaagagc cgagaaaacc gtcagggcta cgacgaccag ccctgcaata
     6661 aaaacgaaca ccttgatgaa gatacccatt tattccgtca accctattgg cagttcgtac
     6721 atccgttttt gatcgtaatt cttttcgaga atgaccagtc tgtagatcgg accttcgccc
     6781 gtaaaggaaa gctggaaatc gggcgcaact tttttattga acacggtaaa gatatgttca
     6841 cggccaaggt tatacaaggg attcaaatat ctgtccccga tccgtgcggc gtagacaata
     6901 ccatcgaacg agacgtaaat aaggaagtta ttgttggggc caacggcata cacccccttg
     6961 gccagcgggc caagttgata ttccaatcca tcgtcacagc gcaggatgtt gagaatatct
     7021 ccttttttgg tgtcatccac tttgacgaca ataccggggc agcccgtcca ctgtgaggtg
     7081 ggagtgagcg tgggaatgct gctatgaaaa agtgtgaccg ttgcagtggg cacgatcgta
     7141 ggaatcagag tgggggtggc tgtcggttca acagggggag gaatgacaaa agttggtgtt
     7201 tcaacgatgg caactggcgg cgggctggcc tctggcggaa caacgcccag gttgcaggcg
     7261 gttggcaagg tcaacaccac caatgcaaat actaaaaaca gagaagactt gccaattcgc
     7321 atgacacaac ctctttcttt ttattcagca gattcattgc aaaaaattat agcatcaaaa
     7381 aagcaggctc aactttgggt tgagcctgct tcaattttac acacaaaaac ttattggaga
     7441 tatttttcga attcggccac cagatcttcg cgggaggtca ggccaatgaa ttttttcaag
     7501 acattgccat ttgcatccaa caggtagagt tcgggctggt aataaaaacc gagcgcgcgc
     7561 tggaatccgt ctgtgcgggg gtcatcagca tcgagatatg aaaacttgac cttgccaaaa
     7621 tactctgctt cgagcccatg aaccatgggc gccatggatc ggcaggtcgc tcaggtgaag
     7681 cggaaaaatt ccaccaactg taactgacca gccgccaaat tgaccgtggt ggggtcggtg
     7741 gcttcgaggt tgggtccgcg ggaagtggca acgggcagca cttctgcgac ggcttgagtg
     7801 ggcacagggg cggccacttc acctgtggcg gtgggaatcg cttccgcggc gggggcggtt
     7861 gttgccgtag gaagttctgc cgctgggggc gggttggtcg ccgctgccgg ggcgcaggcg
     7921 gatgccgcaa aaacggcaac cagcacaacg gtcaggaacg aaccgtgtct ttgaatccga
     7981 tctggaaagt gtggtctcat gaataaaagt ctcctgatgg tatgttcaat atgataacgt
     8041 gatttcaggc cttcttacaa aatctcatga aattcttagc cgcgcctgaa aatggctcta
     8101 ctttgacgat tgcatcgggc tgcgctatac taatattagt tcattcattg gctggctgtt
     8161 aaatgatggt cacagtcagc cagctaaaaa ccgttgacca tcaatccaac cccggaggat
     8221 ctttatggca gactttaaac ttacatacgc aaccatgttc aacccacctg aagaattgca
     8281 taccggcttt gacaaagccg tggaaaaagt aaagaagaac atgggtcagg aatacaccat
     8341 gctcatcaac ggcaaggagg taaaagccga cgacaaattc gatgacttct cccccgtcaa
     8401 cacggactgg aaacttgcgg tcatgcagaa aggcaacgcc aaacatgcgc aggaagcgct
     8461 ggccgcggct cgtgcggcct tcccaatgtg gagccacacc ccctggcaaa agcgtgtgga
     8521 gttgctccgc aaggccgctt ccctgatcga agaacgcatt ttcgatctcg gcgcagccat
     8581 ggcgcttgag gttggaaaaa accgcatgga atcccttggc gatgtgcagg aaaccgccga
     8641 cctcatttac tactcctgct atcaaatgga gaagaacgat ggctacatcg ttgaaatggg
     8701 caaagacccg ctggttggat acgatgcccg caatgtttct gtcctgcgac cgtacggtgt
     8761 gtggctcatt gtttctccgt tcaacttccc atttgcgttg acaggcggcc ctgccggcgc
     8821 ggcgctggtg accggcaata ccattgtcat caaacctgca accgacaccg cctggatcgt
     8881 ccgcctgctg gtggattgtt tccgcgatgc gggcatcccc gatggcgtcg tcaatttcgt
     8941 cacggggcct ggctccacac tcgggcaggc gcttgtggat agctccgaag tggatggcgt
     9001 caccttcaca ggctccttcg atgtgggcat gaagatgttc caggatttcg gcaaacgcaa
     9061 ctatgtgcgc ccgatcattt tggaacttgg cggcaagaac cccgccattg tttcgaagaa
     9121 tgcggatctg gaacgcgcca ccattggcat catccgctcg gcctttggtt tacagggaca
     9181 gaaatgctcc gccgcttcgc gcgtactggt agaggattct gtttacgacg acctggtcgg
     9241 ccgtttgaaa gaagccgctt ccaaactcgt cctcggtgac cccaccgacc gcaacaccta
     9301 caacggcccc gttgtaaaca agtcatccta caatgatttc aagaacttct gcgaagagat
     9361 caaccagggc ggcggcaagt tcctcgtcgg aggcaacgtg aagaccggcg gcatgttcga
     9421 caagggctat tactgtgaaa gcaccttcgt tgttgatctg cccttctcgc accgcttgtg
     9481 gaaacatgaa atgttcctgc ccatcaccac cattggcaga gtcaagaatt tggatgaagc
     9541 catgagcatc gccaacgatg tcaactacgg cctcaccgct ggtttctacg gctcaaccga
     9601 agaaaccgaa tggttcttcg ataagatcga agcgggcgtc acctacgcca atcgtccgca
     9661 gggtgcaacc actggtgcat ggcctggctt ccaacccttc ggcggttgga agggttctgg
     9721 ttccagcggc aagaacgctg gcggccacta ctatctcccg ctttacatgc acgaacagat
     9781 ccgtacactc gttaagtaag aagcataaac acaaacccct ttctcaactt ttcgagaaag
     9841 gggttttcaa ttatctatag gtattcatca tgccctggga ccccactcaa tatcacaagt
     9901 ttcaagcgga acgctccgcg cctttctttg acctgttgaa actggtcaac atccgcccca
     9961 acctgcaagc cgtggacctc ggctgcggca ccggcgagct caccaaacac ctcacagagg
    10021 ccctgcctga aggcaccgtc cttggcctcg attcttcgtt ggaaatgctc aacaaagcca
    10081 gacaacatga aggcccttcc ctgcgattcg agcagggtga ccagtccgaa ttgcaaggcg
    10141 actgggatct gattttcagc aatgccgccc tgcaatggtc tgaaaatcac gaggcgctga
    10201 ttccgcgttt gtttggcagg ttgaaggaaa acggtcaact tgccgtgcag gtgccttcca
    10261 atcattttca tatctcccac cagatcattc gtgaaacagc cagggaagaa cccttccaaa
    10321 ccgcgcttgg cggatttaac cgtcaggcgc ccgtcctttc catcgaacgc tatgcagaga
    10381 tcctctttga atgcggcgcg aaagacatca ccgtattcga aaaggtatac ccccacgtac
    10441 ttgaaaactc cgacgccatc gtagagtgga ttcgcggcac ggcactggtg ccctaccttg
    10501 aaagattggg ccagcaaaaa gaagctttca ccgaaaccgt ccgcacgaaa ttgaagtcgg
    10561 ctgtaccggg cagtcctgtt ttctacccat tcaaacgcac ctttctttct gcggtcaagt
    10621 gaagcagcct ttggtagaat acccccatgg acgatgattt tgtctttcgc atcggcatgt
    10681 tcttctatgt catcggcggc gggtcctttg ttctctttgt cacctccgat atcgccgaca
    10741 aggtggattt tgactttcta ttcgtcgccc tgctgatgtt tggaatcggc gcctacctca
    10801 ggcgcggaat ggcacccccg ccatcggctg ggcggttcgc ctccttcaaa aagtggcgcg
    10861 aaaatgcgaa aaataaaaaa cagcaaaaac aagaggccaa gaaaaaataa catgaccaaa
    10921 ctcatcacac tcaaacaggc aatttcagat tttgtaaaag atggcgacgt ggtatacgcg
    10981 gcggggttca ctcacctgat tccatttgca gcggggcacg agatcatccg ccagggcagg
    11041 aaaaatctca ccctcgcccg cgccaccccc gacctgatct acgaccaaat ggtggcggca
    11101 ggttgtgcca aaaaagtcat cttttcttac atgggcaatc ccggggttgg gtcactgcga
    11161 attgtgcgcg ccgctcttga aaaaggcgaa ttggaatggg aggaatattc ccatttcgga
    11221 atgatcaccc gcctgcaggc gggcgccgcc gggctgccat ttctccccat gaatcaaacg
    11281 ggagcaaccg acctcgaaag atcgaacccg ctcatcaaac gcattcccga cccgtatggc
    11341 ggcaaggatg tcatcgtcgt gcctgcgctt aatcccgatg ttgccattgt ccacgtgcag
    11401 cgagcggata agaacggcaa tgcccattta tggggcatca tcggcgaaca aaaagaagcc
    11461 gcctttgccg cgaaaaaggt catcctcacc gcggaagaga tcgtcgaaga atccgtcatt
    11521 cggtccgacc cgaaccgcac gatgatcccc gccaatattg tggacgcggt ctgccatgtt
    11581 ccgttcgcaa gccacccatc ctactcacag ggctactacg accgcgacaa cgagttttac
    11641 ctcgcctggg ataagatcag cgagtcacag gattcggtca aggcctattt ggatgaatgg
    11701 gtttttggtg tgaaggaccg cgaagaatac tggcaaaaac ttggctctga aaaacgcgaa
    11761 caattgaaag tggtcagcgc gttgagtgag ccagtgaatt acggaaaata ctaaaataaa
    11821 cgcggactca agtccgcgct caaaaaataa aaacgcagat cattgctgtc atgtgcaagg
    11881 tctgcgtttt tctgtattca tcgacaaccc agcgtcgttt tattcgtccc tgcttacgtc
    11941 tccaaatttt gcatttgtca aacttgccag atcggccact ggcaattgaa tatccaaccc
    12001 acgctgaccg cctgaaatat agatcttctc aaacccctgc gccgcagaat cgatcaccac
    12061 ttgaaagccc ttgttgatga gcgccagcgg agaaatgccg cccgcctgca ggcccgtcaa
    12121 ggcttcggct tcgcgctcgg tgggcaggga aacttttttc tcgttcaaga acgctgccag
    12181 tttttttaga tccaccaccc gcggcccggg gatcaccgtc aatagcggtt tctttttttc
    12241 acgggtaacc acgatggtct tgaaaacctg ttcgggcggc acacccatat gctgcgccgc
    12301 ctccactgcg ccgagtttct caggcgggag ttcatgcgcc tcgtacttca ccttgcgcgc
    12361 atccaaaaat cttgtgacat tattggtgac tgtcataatc aaaaaatcct gtacaattat
    12421 aagcataaat tggatgaacg aaccgccgct tcaaaccctg accgtacacc tgctcctgcg
    12481 aaattgatat tttcagccct cgggcaaatt caaattattg gaggccagtt atgacaacag
    12541 caagcgtgtt atcccagttc ggcttatttc aggatctacc ccagcagctt ctggaagaga
    12601 tcgccgcgat ttcagatgaa gtcgaaatga aacagggcga gttcgtcttt cgtgaaggcg
    12661 aaaaagcgga caaactgcac ttcctgctta atggcagtgt agccctgcgc gtcaagctga
    12721 cttcgagacc cgaaagcctg accgtttcgt tcgtatccac gccgtatcaa agttttggct
    12781 ggtcgggcat cgttgcgcca aatcattaca cctccagtgc cgaatgtgat gaagactcga
    12841 aactgctcat cacacccgcc caacccttcc tcaaattgct cgagaaatat cccgaagcgg
    12901 gcttcaaagt gatgcagcgc atcgccgaga tcatcgcaga ccgtttgcgc aacagcagac
    12961 aggccttgat caagaccctc taaaatcgct gattcgccaa aagaaagcag gaccatgcct
    13021 cggttaaggg ggaagcgggc aggtcctgtt ttttattaat tccgcgtagt acaataaagc
    13081 gcatgaaatc gtcgaatact gccattccac ttgaaaaact catcgaacaa ttaccagagc
    13141 aatataccgt tgcagaccgc gaactggttc agcgcgcata tcgggtggcc gaagaggcgc
    13201 atcgcgagca gaagcgaaac tcgggcgagc cgtacatcaa tcattgcatt gcagtggcca
    13261 gcattttatc ggacctgaaa gtgccgcccg aagtgatcgc ggcggggttg ctgcacgaca
    13321 cagtggaaga taccaccatc acgctcgcgg acatccgccg cgactttggg gatacgatca
    13381 aaattttggt ggacggcgta acaaagttaa ctcacctgcc gcgggtttca cgcggtgacc
    13441 agcaccctga aaaagacgaa aacggaaatc acactgaaga aaaaccgatc gagcctgccc
    13501 tgcttggccg caaggaagat attgtttcag agaccctgcg caagactttt ctggcaatgg
    13561 gcgacgatgt gcgcgtcatt ttgatcaaac tggcagaccg cctgcacaac atgcgcacgc
    13621 tgggccatat gcctgagcac aagcgcagac gaattgcaca ggaaacgctc gatatttttg
    13681 ccccactggc aaaccgcctc ggcatctggc agatcaagtg ggagttggaa gacctcggct
    13741 ttcggtatgt gaaccccgaa aaatataaag agatcgcaga actgctgacc gaaaagcgcc
    13801 ctgaccgtga agcgcagctg gaagccatca aggaaaatct ggtcaagctg cttgaaaaga
    13861 acaacatcaa agcggaagtc agcgggcggc ccaaacacat ttattcgatc ttcaagaaaa
    13921 tgcaaaagaa gggcaagccg ttcgacatgg tgcgcgatgt gcgcgcggtg cgtttgatcg
    13981 tgcccgatat gccatcctgc tatgcggcgc tgggtgtcat ccacaccaca tggcggccca
    14041 tccctggcga gtttgatgat tacattgccg caccgaagga taatttttat caatcgctgc
    14101 acacagccgt gatctacgat gataagcgtc cgctcgaggt gcagatccgc accaatgaga
    14161 tgcacctcaa tgccgagtac ggcattgctg cgcactggcg ttacaaggaa ggcaccaagc
    14221 acgcagacag aaattacgaa caacgcatca attcactgcg caccatgatg gaatggcgct
    14281 cagacgtgaa agacgccgcc gagttcgttg aatccatgcg cagtgatgtg ttccaggatc
    14341 gcgtgtacat cttcactccg cgtggcgaca tcattgacct gcccgcaggc tccacaccaa
    14401 ttgactttgc ctatcacgtc cacaccgata tcggtcattg ctgccgcggg gcaagggtga
    14461 acggaaaact ggttccgctg tatcaggaat tgaaaacggg cgatcaggtg gagattctca
    14521 ctgcaaaacg aggcgcgccc agccgcgatt ggttgaaccc gaatctgggg ctggtacgca
    14581 cccagcgcgc ccgctcgaag atcaaggtct ggttcaagaa gcaggaagac gagcaaaatc
    14641 tcgctcaggg acgtgacgct ctcgaaaagg aactgcagcg cctcggcctg caagacatca
    14701 acttcgaaag gatggcacgc gacctcgaat acaagacgcc cgatgaaatg ttcatcgcgc
    14761 ttggcaacgg tgacctatcc gtcagcaagg tgatcaagca gttctcacag caagaggcgg
    14821 ccagcgatat cttcgaggtc aatccatcca ccacaccttc cacttccaca aatgccattg
    14881 aagtggtcgg cttgaaaggg atgctctcca caatggcgcg gtgctgcaac ccgatgcctg
    14941 gcgatcaagt ggtgggattc attacccgtg ggcgcggcgc caccatccac aggcaggatt
    15001 gcccgaatat tttgatcctg caaacaaagg aacgtgaacg cctgctgcaa gttggctggg
    15061 gtcatgtaga acggacctac cctgtgccga tcaagatcaa ggcctacgac aggcagggtc
    15121 tggtcagtga catttccaac ctgctggcgg atgaaaacat caacatcgcc gatgtgaaag
    15181 tgaacgtcaa ccgcagcatg gcagatttgc gcctcatcat tgaggtcaaa gacctcactc
    15241 aactcagtcg cgtgttaacc cgcattgaaa acctgcccaa cgtgatggaa gcacatcgcg
    15301 tcaactcggg gtaagtaaac cttttcctgt tgcagatgaa aagcaatttt tgggagaaat
    15361 cagcagacat cctgaggcgt aacgctgccc tctttgcatt cctgcttgcg ttggggctgt
    15421 atggattaag cgcaggaata aaactcacac agcaatcaaa aacgccgcaa tatgtatatc
    15481 tggcttactc gttcctgcaa ggacatactg acctggttga actaccagaa agcagatatg
    15541 acctgatcga attcaaaggt aaatggtacg tccccggggg aatcacgcct gcgcttcttt
    15601 cgcttccgtt tgtgatctta ttcggcaagg gtttcagtga tgtgcttttc ggtgtattgg
    15661 tcggcgcaac aaatgcggcg ctgatggctg tcctgcttgg ccaaattgta gaagacttct
    15721 ccacgcgcat ctggttgatc gcgttattca tcgcaggcac cgttcactgg tggcttgcga
    15781 gtgtgggatc ggtctggttc aacgcacaga tcgtggcctt gttgttcatg ctgttatatg
    15841 ctcgggatgc cattcgaaac agaccctggt tggctggctt gtggttgggt ctcgccttcc
    15901 ttgcccgccc gccgacgatc ttttctgcat tgttcttttt gctcctcgtc gttctgcgcg
    15961 aacgctccat cacccccgtg ctaaaaaaga tgattccctt cggggtgatg ctggccgcaa
    16021 gcgtcgctgt gatgctcacg tacaaccaac tccgcttcgg caatccagtg gattttggtt
    16081 atgggtatgt ccgcggttcc aatgcgttga tccaaacttt tgcgcgcagc ggcggattca
    16141 atgtgcagta catgccttgc aatatccatg tttcgatttt cggtttgccg aacctgaata
    16201 caggcatttt tcccagcgtc aatgaagcct gctcgtatct tgagccgatc agccatgact
    16261 ttggaaagtt atccaaattt ttcaacccat tggggatgag cattttcctc gccacacccg
    16321 cttttttgct gatttttcga gcgaagcgcc gtgatgactg ggtgatccct gcctgggcgg
    16381 ggatgatcgg gatcttaatc ccgctttgga tgtatcatgc cacaggctgg gtgcaattcg
    16441 gctatcgtta cacaaccgat ttcatggtct ttcttttcat cctgctggcc agcgccatca
    16501 aggaaatccg ctggatggaa aaatcactga ttggcatgtc tgtgttcatg ggtggaacgg
    16561 gaatgtactt gatgtattac atgacattcg ggctggtatg gcctgaaatg ttcatggagc
    16621 ttgcgcgaaa aatttatcat ttcattttct gatatgaagc gacaaaaatt caacccagtt
    16681 acgatgacga ttttgctgct ggtgtttgca tcagtggcat ttcgcgtaca ggcaattcca
    16741 aactcaaatc gtgatatggc agtgtacaac ctgcgctggt atcaggagat aagtcaaaaa
    16801 ggcatcggca atgcactggg gacgaatttc tccaattatg cgccgccata tacttatttg
    16861 ctggccctcg ccacgctgac ccgtgatctc attccgccgt tgacggcgat caaactcatc
    16921 cccattttct tcgacctgct cggtgcatat ttcatttaca ggatcgtcag gctgaaatat
    16981 ccgcagggga atctgtccat gctggcggca gcgatctatt tttccgcgcc gaccgtggta
    17041 ttgaacagcg cctattgggg gcaggcagat tcaatttata catcagccct gctggcctgt
    17101 ttatatttcc tgttgaccga aaaaccgctg cctgccatgc tggcatttgg cgccgcgttc
    17161 tcagtgaagg ctcaggctgt ttttttgctg cccttcctcg gggtgatggt gcttcgaaaa
    17221 aaaatccact ggtgggtcgt gggtgttatt ccgctggtgt atctgctggc cattgctcca
    17281 gttgtgttgc tcggcaggcc attgatggac gctttactgg tgtatgcaaa acagtcagat
    17341 acattcaaca tgctttcgat gaatgcgccg aatatataca gcctgtttca gcgtgagtgg
    17401 tacatggtgg ttctgccttt cgggctggta ttgacagcag cactcactct cggctggatg
    17461 tactcctcct ggcaggcaaa aacagacttg gacgacaagc acatggtttt gcttgcattc
    17521 gtttcggcaa tgttgattcc ctttttgctg ccaaagatgc acgaccgtta tttttatcca
    17581 gccgatgtgc tttccatcgt gctggcgttt tactggcccg ccttgtggtt catgcccatt
    17641 ctcttgcaga tcagttcagg cggggcgatt gcggtcttcc tgtttggggc tgatccagct
    17701 ttcacggtgt atggtttttt gttgaactcc atcgcgctga ccattgccat gcacacccaa
    17761 cgggtcagcg aaaaacgaca aacgcatccg ctcgtttcat cttcgctggc atggctggct
    17821 gcgtttcttg taccccttgc cttgttcgga atcggggcgc ggctgctgct cacaccaacc
    17881 ttcatccgcg cggaatatgc tctgccgtac acgcaagaag cgcagggatt gagcaggtca
    17941 gagcgttttc aacaggcttc gcaaacgctt gaatacatgg gcagcgacag aaagccacaa
    18001 tttctacaaa ggctcaggct cgataacggc gcacccgttt ttaccgaaca ggagatcacc
    18061 acgcttgaaa gcgtgaagca gtccttgcga acgacattct cgatctggtc tctttcgctc
    18121 gcagttctct ttctcctcac cctgctggct caagctgggg aatggctttc aaaacttcgt
    18181 catggtgtac agcgcggcgg atggatcacg gttgcagttg cggtcatttc tgctgcggcg
    18241 gtgattttcg ttcccataaa ggtttttgaa ggcgaaatgc tttcaaggct atttccaaat
    18301 acgctttggc gggatgcagt tttaattctc tgtttgttca tggcggcgag cggatttcta
    18361 cttgcaaagt cactatccac attgggtgga aatacagcgt cttcgcaatg atggcataag
    18421 tttgaatgcg ttataatgcc gtggctttta gccatgtact cagttattga agcgcgcgag
    18481 cgcatcctct cacacttcga aagcaccccg catgaaagtg ttccgctggt ggcgtgttct
    18541 ggccgtgtgc tggcggtgga tgtgactgcg gcgcatgacc tccccccatt cgataactca
    18601 tccatggatg gattcgccat ccgctctgca gactcggcta acgccgccgt tcagggctta
    18661 accttgagcg tggttgctga tatcccggca ggctcagcgc cgaaggtgac tctcgccaca
    18721 ggccaggcgg cacgcatcat gaccggggcg caactgcccg ccggtgccga tgctgtcatc
    18781 cccgtggaag acaccgactt tcatgtgcgc gatgctggca ccaccccacc cgaaaccgta
    18841 tcgttttcgc ggaaggtcac agcaggtgaa aatatccgtc cgcgcggcat ggatttgcat
    18901 gccggggacg tggttctaaa aaagggacgc aaactcaaac cacaggacct gggtttgctg
    18961 gccatgcttg gcatcgccaa agtggatgtt cacaaaaaac cgagagtggc cttgctttct
    19021 tcaggcgacg aactgttgga agtggattct ccgctaagcg aagggaagat ccacgattcg
    19081 aattcgtatg cactggcagc cgcttttgag tctgtagggg cagaagtcat tcggcttggg
    19141 gtggcgaaag atacgcgcga atctgtggaa gccttgttga aaaaatcaac tcttgaacat
    19201 gcagacctga tcgtgtcgtc tgccggggtc agcgtgggcg cttttgattt tgtaaaagat
    19261 gtgatcgaaa caaacggcaa actcgatttc tggcgcgtga acatgcgccc gggcaagccg
    19321 ctggctttcg gctcttacaa gggactgccg ttcattggac tgcccggcaa ccctgtttcg
    19381 gcgttcgtgg ggttcgaggt ttttgtgcgg cccgcgcttg agaagctgag cggtttattg
    19441 gacgggacca gactcacagt taaagtgcga tgcggggaag tcatcgaatc agatggccgt
    19501 gaaagttatc tgcgcgccaa tatccgtctt gagaatggaa tccaaaccgc atttttgacg
    19561 ggacaccagg gttcggggaa cctgctttcg ttggtacagg cggatgcttt acttattatt
    19621 cccgctggtg taaaatgcgt gcctgttggc caggaagttg aagcattact tttgtgagga
    19681 aaaatggata aatggttata ccgcgcatct gttgcgcttg tggtgctggg gctgctggtt
    19741 tcgatctaca tgaccatcta taaatatagc gggaatgaca atatgtgcct tggctccggg
    19801 gactgccata cagtgaatgc cagcaggtat tccgaggtaa acggaattcc cgttgcggtg
    19861 ttcggcatcg gtggttatct ggcgatcctg gctgtgttgt attttgaaaa ccgtaatcgg
    19921 ttctttagag agaatgccac attgatggtc tttggcatgg cacttactgg ttttcttttt
    19981 acggtctggc tgatctatgt tgagatcgca ttgttgaaag ccatctgccc gttctgtgtt
    20041 acttcgcagg tttcaatgac gctgattttc attctagctg tctggcgcct gatcagacaa
    20101 ccccaatctt aggaggaagc gaaatgcccc gtattaagtg tcactacata gattgtgtgt
    20161 tcgtggacga agggtattgc tctgccgcgg cggtggagtt tgatcccgat acaggttgta
    20221 acacctattc cgccgccgat ggtgtagccc caaaggatga cgactgggac gaagaagatg
    20281 aaaccgaaga gtgggacgac gacgccgaag aagaagaaga cgatgactgg ctcgatgacg
    20341 aggaagatga gttttagtcc gcttcttgct taactgaaaa ttgaaacttg aacaaaaagc
    20401 tttctctgcg cgaggaagct ttttgttttc agacaggaga tttccatggc tgcgttgacc
    20461 tgacttgcgc ctgtgcaata taatcggctt gactttattt gaaagggaaa tttcacaaaa
    20521 caaataaagt aaccactgga ggaaattcag aatgtcaaga aaaacaataa tttattcaat
    20581 atcagtaatc atgatatgca cagggcttgt acaaatttgg aggttctttt cacctctttt
    20641 tggagcgggc aagtcaatga atttaactct tatcttaagc ggcgctttcg ttttgtccgc
    20701 ggggtggaac ttgttaaaac tcaacacaac tggaagagaa ttggctttct ggattttgtt
    20761 catgaacctg cttggcggtt tgctggtgct aggctttatt cttccagtca acggtatagt
    20821 ttcgatcaat gtacagtttt tagataagcc cgttttcaac tctgtggatg aacgtctctt
    20881 ttcaatcgtt tttctggccg tatttcttgt catcgattta tcagccctcg tttttctgga
    20941 acaggagaaa acgaaaaaac tatttgcgcc cgaccttgtg gaaaatctca cctcaaaagc
    21001 agatgccgaa tcaaaatcaa catcagaaaa ataatgatct gtgttgcact cggaaattag
    21061 agcagagaaa tttgaccagc tatgtgccag cgcgtataat aatttcatgg aaaccaccaa
    21121 atcaaaattc gcttccattg atgaatacat tgcgacattc cccaaagaaa cgcaaaagct
    21181 attgaatgat ttacggacag ccataaaagc ctccgctcca aatgcggagg aaaaattcag
    21241 ctatgggatt cccacttttt tcctaaacgg gaacctggtt cattttgccg ctttcaaaac
    21301 acatattggc ttttatccag cgccaagcgg aattgaggct ttcaaaaagg aattatccat
    21361 gtacaaacag gcaaaggggt ctgtgcaatt tccattggat gcccccctgc cgttgaaact
    21421 ggtcggaaaa attgtcaaat tcagggttgc tgaaaaccta aaaaagacaa gcaagaaaaa
    21481 atgacttgcc ccccgccagc ttcccgcttt tctactcccc aaactgatat aacaaaactc
    21541 ccgcagccac agcaaggttg agcgaactga cacggccttt cataggcagg gagacggtca
    21601 catcacaagc ggcagattgc tctgtggtga gtcctttttg ctcattgccg agcattaaga
    21661 tccatggggt gtgcggggta agggtgtgat gatccacatc tgccttggca gatgtgccga
    21721 tgagttgata cccgcctttg cgtgcccagg cgatgaaatc atcaaaggag gcttggacaa
    21781 tgggcttcca aaacaaggta cccatactgg cacgtaccac ggtgggatga tacagttcca
    21841 cgccgccttc aagcaggaag agaatttctg caccgaccgc atccatggtc cgcagaatgg
    21901 tgccgacatt gccagggtcc tgcggagaga cgagggccac agcgctggaa aatttcttga
    21961 ggtcggtcag ctgggtttgg cgggaacgaa ccagcgcaat gatgccttgt gggttttcct
    22021 tgtccgccag agattccatc acagaaacag aaacaggctg gggcggcgtc ttgaggcgtg
    22081 taagcaggtt gcgcgcaaaa gaactggtca ataggtcagg ggcgtacaaa accgtttcga
    22141 tcttccaatc cgcttcgatg gcttcaccca catgatgaat gccttccaca aggaagagac
    22201 ccgtttcaag gcgggtcttt ttttggcgca gagctcgggc atgtttaacg agcggattgg
    22261 caaggctggt gatcatttcc atgcgccgat tgtatccgaa aaccaaacag cggatggcag
    22321 gagccatccg ctgtttgaag tgctgatcat ctacggggcg gcaacgacca gtgtgccagc
    22381 catgcctgcc ataaaatgac ccgcagtgcc acatacaacc tgatattcgc cagaatcggc
    22441 gggtgcagtg aatgtggtgg tcacggtttt gccaggctca acctcgacct cccagtaaat
    22501 attgccttca tcctcatcgc caaaatcatc gcccacggtt tgcccaaatt tcatgatgac
    22561 aaactcatgc agcaccgcac cattgtttgc aaccgtgatg gtgatctcct cccctgccgg
    22621 gacgacaaat tcagccgggg tgaaggtgaa ttccagcata tccactctta aatttttgga
    22681 tggcttgttc ccgccacagg ctgtgagcag aaaaacaagc acaagaaaaa caacaacgat
    22741 ctttttcata ttaaagctct cctctttggt attcaggttg aagtcagttt acatactttc
    22801 ccgacaattt tcaatgggaa tatttgtctt ccctgttcat gccgtattga gtaaactgca
    22861 aagtgtattt tcaaaaagga gaccaaatga atactctggt gcaaaaatcg aatacagccg
    22921 tgtactggca atatgcctta tggcttgcca tatttactgt tttttataac attgcagaag
    22981 ggctggtttc catttcattc ggtgtcagcg atgaagcact gacccttttc ggtttcggtg
    23041 tggattcttt cattgaggtg atttccgggg cagggatcct tgccatggta ttgcgcattc
    23101 ggcaaaaccc gaatacatcg cgcacccgtt ttgaagtcac tgccctgcgg atcacaggca
    23161 cctcttttta cctgctggca gtggggcttg gcgtcagcgc catttacaac ctcgtcatcg
    23221 ggcacaagcc agataccacc ctgcctggca tcgtcatttc ggtcatttca attgcagtga
    23281 tgtgggcttt ggtaatgggc aaacgcaaaa caggtcggat gcttaattcg atgccgatac
    23341 tggcggatgc gaactgcacg atggtttgca tttacatgtc ggtcattctg ctcatctcga
    23401 gtctggtcta tgccgtgagc gggttcggtt tcatagacag tatcggcgct ttgggtttga
    23461 tctacttttc atacaaggaa ggacgcgaag cctttgagaa ggccaacggg ctcgaatgca
    23521 cctgcgagga tgaacactgc aaggattaag ttaagggtgg tttataatta tctgcatgaa
    23581 atttctacga tggttggcgc ttgcggccac gatgctgttg agcgcctgcc agtcaaaccc
    23641 ttccccatcc atcacaatca ttgacagtgg tcagattcag gtcgttgcat ctgattcacg
    23701 gataccgctg gtgctgctca cccaaaacgg gatcaccccg cagcaatttg accgtgtgct
    23761 ggtgaacggt gttccatttc ccatagacca accattgcca tccgttgatt ctgtccaatt
    23821 gcagttacgc cgcgcagtgg atttgacttt gaccacgccg cagggaacgg aaaccttcca
    23881 aacctccgcg ctgaatgtgg ggcaggctct ggccgaagtt ggataccaga tcggcgcagc
    23941 agatgtgctt gacccgcctg cagagactcc catcacaact gcaatgaatg tgacctttac
    24001 accagcccgc gatttgacgg tgcaggtcgg cgaaagcgtg gtacacatcc gctcggcggc
    24061 tgggacggtg ggcgaagccc ttgccaaagc ggggattccg ctcatcggtt tggataccag
    24121 tttcccgtta agtggtgaag cgctgcccgc cgatggtcag attcaggtgg tgcgagtcca
    24181 ggaaatcatt gatgtggcga tcgagccgat cccgttcgaa acaaaaaaga tcagttccat
    24241 tgatgtgccg ctcgggcagg aggagttggt gcaatctggc gtcaatggca ttgcaatgac
    24301 gcgcacacgg attcggtatg aaaatgggca ggaagtgagc cgcatccttg aagaagagat
    24361 cgtggcgcgt gagccgcagc cgcgcatcgt aaaaagcggt gaacagatcg tcatgtcgcc
    24421 tttgcccggt gtttcgggcg aatattggac ggcagtggaa atgtacgcaa cggtgtattc
    24481 accctgcgaa tctggcacag gcggatgttc ttacagcact gccagcggcg cgcgcgcagg
    24541 gtacggcatt gtggcagtgg attattcgat ctacagttat cttgcaggga tgcgcgttta
    24601 catccccggt tatggaaccg ccaccatcgg cgatacaggc ggcgggccga tcatcgaaac
    24661 cgcattcggt gtgccgcgca caaaatggat cgacctcgga tttgacgatg gcgcaattca
    24721 ggatatgaca ggctgggtga cggtgtattt ccttgcaccc gcaccagccg aggtgccgta
    24781 ttttttgaag taaaccttgc gccagcaaat gacacagatt cttctcttgg aggatcggcg
    24841 tgttattttt tactcactgg caatttgaaa ttcccactta aaggcagaga gaagaaaaca
    24901 gaacatgacc caaagccaaa aaaggatact cgccattctt tcgtttgttg cgtttgtgtt
    24961 ggtggtcact gcaggttttt ttgcatttag aagttaccgc gccttcgtaa cccagccgct
    25021 tggcccgtca ctgccgatcg ttgcacagac catgccaccc acctggaccc cggcggccac
    25081 cacatcgccc aacatgatca cactggcccc aaccctttcc ttcaccaccc ccactccata
    25141 cgctggctgc ggcggaacca ccagcgttat gcatgtggta gccatcggcg cagactcacg
    25201 ctccgataat tatcaatacg gccttgggga tgtcatccgc cttgtacgca ttgatttcgt
    25261 aacccccaaa gtttcggtgc tcgaatttcc acgtgatttg tgggtggaaa tcccatacat
    25321 ctctgataac ctcaatgggc aggatcacga aaaactaaat caagcctatc tttatggcca
    25381 acctggcgat ggctttcatt attgggatga tcccagcgca ggcccgggct tgctctctct
    25441 cacgatcaac ctcaacttcg gcgcgcaagt cgatcactat gtggccgtca acatgcgcac
    25501 ttttgtaaac gtggtcaacg ccgttgaagg gattgacatc aacatccccg atgaaactgc
    25561 cgcagaaaat acaggcctca caatcggcaa acatcacctc aatggcgatc aggcgctgaa
    25621 ggtggcacgc aaccgtgaag aaggcgtctt cgaacgggcc agtaaccaaa accgagtttt
    25681 gtgcgcgttg cgtcaaaagc tcgccagccc cgccgtcgtc accaaaattc cagaattgat
    25741 cgattctttt caagacaaca tcctcaccga tttcaccccc gaacagatcg cccagctcgc
    25801 ctgcctcggc acaaaactgc cgcctgaaaa catcgtcttt gccagtttcc cgcaggagct
    25861 tttcaaagaa agccgcatct acgatcccgt tttcaaaaag aacgttttct actgggatgt
    25921 ggatttcaac atcctgcgcg actacaccgc aaagtttcag gcaggcacat ggcccgaacc
    25981 caacaccatc accaccgatg aagaagacga agaagagatc atctgcgaat gaccgattac
    26041 cgttccattc cgccgctgga tgcccacgcc gttctcaaac gctttcaact gcgcgccgat
    26101 aaaagcctcg ggcaaaactt tctgcaggat tcctccgctc ttgaaaagat cgcccgcgcc
    26161 gctgaaattc agggcgatga ctgtgtcctt gaaattggcc ctggccttgg cagtttgacc
    26221 cgttacctgg cagcctctgc ccaaaaagtg acagcggtcg aactggaccc tgacctgctt
    26281 gccccgctac gggcagttct ctccccgtat caaaatgtca acgtgatcca tggcgatatt
    26341 ctcaagctga acatctccga gatcatcgaa caacccgatt atctcgtcgc tgccaacatc
    26401 ccctacaaca ttacctcagc catcatccgc cacctgcttg aaagcacccc caaaccacgc
    26461 cgcgtggtgc tgaccattca aaaggaagtc gccgagcgca tctgtgctgc acccggcgac
    26521 ctgagtttgc tggcattaag tgtgcaggtg tacggcagac ccagcattgc cgcaaaaatt
    26581 cctgctgggg catttcaccc cgtccccaaa gtggattccg ccattctgcg aattgacatt
    26641 cacaaggaac cgctcatccc tgctgaattg ttaaatacct tcttcaaact gatcaaggcg
    26701 ggtttttctc aaaagcggaa aaccctccgc aattcgctat cctctggcct gcacattccg
    26761 ccgcatgaag ccgagtccat gctcaccgcg gcagggatcg atttcatgcg ccgcgcagag
    26821 accctttcca ttgaagaatg gagaaggttg tgtggttaat acaattcatc ctcgtccacg
    26881 ggttcggcgg gttctgaatt caatggatag gcccagatgc ctttttcgtt caaggctttt
    26941 gcgatctggt acggcttggg cagggcatca tagctttgcc cgctcataaa atccacgacc
    27001 agttgatgag ggggtctctc agttttgaaa ctcttctgaa cgcgccccag aaagaacttg
    27061 gcagtctcat ttgagacctg aaaacgtgcg gcaatgacct cggagggttt gatcttttta
    27121 tccatgagat tctcctgcct gattgtacct tgttgagaaa aacacactgt tcctgagatc
    27181 aatccacaaa agagcggatc tccgcaagga caaggtcgct gtgctgttcg ggaaaccagt
    27241 ggttgaattg ttcgaagagg acaaatcgcg catccggtcg tgaccgtaac gcctgctcaa
    27301 acctcgccgt ggaggcgatg cggtccagtt ttgtccgcat gtacaggatg gggaaatcaa
    27361 aatcaagata ctctggcttg taaaaaagaa agaacaaggc ttgcagaaaa atggtgatca
    27421 gcaggcttgg gcgatcccaa taatgatagg catccttcca gagttcgtgc gcggtttcag
    27481 aatgataccc cgcccgtgaa acgatggaag cggcaacggt tttgcgtaaa aacggcagat
    27541 accacgccgc acagaaccac agtccgtaaa gccatgtaaa cgcattgagc ccatgttcaa
    27601 aaacatcata acgaaaagag gagccgaccg agagcgcgat caatttttca atgggcggat
    27661 gcttcttctc gcgggcccag cgccatgtat gggtggcgcc ccaatcatgg gcaacaatga
    27721 ccagcttccc cgtcgggctg tttcgggcaa gttcatccat caatgcattg aattcacggt
    27781 ccatcacacc gccagccaat tcacccagcg atggaaaatt cgggttgtcg tgacggtttg
    27841 gaaatgcata ggcgtaaata ctgcgaccct gcagccaggc ttgtcggcgc tcggcctcgg
    27901 tgtgatacgc cgcgaacatg gcaggcgaat cgggaaaccc atgcacaaag acaactgggc
    27961 ttttttgtga atcttccaca aagtgaatat gatttattct ggcatgcatg gcgaatgatc
    28021 tcatttcaaa aaacagcctc gcgaaatatc acgaggctgt tttcatcttt catttctact
    28081 tcaaactttc cctgtgatct aggtgccttc ttcccaggag ttgaggtagt gcaattgttc
    28141 atcgctgagg atgtcgatct ttacacccat cgcttcaagt ttgaggcggg cgatctcgtt
    28201 gtcgatctcg gtcggtacgg agtacacctt cttctcgagc ttgtcggcat tcttcgccat
    28261 gtattcggcg gagagagcct gattggcaaa ggacatatcc atcacggaag cagggtggcc
    28321 ttcggcagaa gcgaggttga tgaggcggcc ttcaccgagg atattgatct tgcgtccgtc
    28381 tttggtgagg tattgatcca caaacggtcg gacaaggttg ggagcgccaa cactcatctt
    28441 ttcgagtgcg gggatgttga tttcaacatt gaagtgaccg gagttggcca ccaaagcgcc
    28501 gtctttcatc acttcaaagt gcttgccatc gatcacattc aagtcaccag tgacggtgca
    28561 gaagatgtcg ccaactttgg cagcatcgaa catcggcatc acctggaagc catccatcac
    28621 tgcttcgagt gccttcatcg gatcgatttc ggtcacgatg acatgagcgc ccataccgcg
    28681 tgcgcgggaa gcgagaccac gaccacacca gccgtagcca gccacgacga aggtcttgcc
    28741 cgcaagcaaa acatttgtgg cgcggatgat gccgtcaatg gtggattgac ccgtaccgta
    28801 gcggttatcg aacatgtgct tggtgagcgc gtcgttcact gcgatcacag ggaatttcaa
    28861 cacgccgtct ttttccatcg ccttcaaacg gatgacgccc gtggtggttt cttcggtacc
    28921 gccgatgacg ttcttcaaca tctgtttgcg ctcttcatcg ctcaaggttt tggcccattc
    28981 agcgagggag ggttcaaggc gctcgaaacg tcccatttcg ataaagaaca gggaggaaac
    29041 gaggtcggcg ccatcatcct gcgtcatgtg gggcttgtgc tccaacgcgg cgcggatatg
    29101 tttgaagtag gtcactttgt cttcgccttt gatggcgaat acggggattt cgtactggtt
    29161 caccagcgca gcggccacat catcctgtgt ggagagcggg ttggaagcgg tgaggacgat
    29221 gtctgcgccg ccttcgtgca gggtgcgcat caggttggcg gtttcggtgg tcacatgcaa
    29281 acagcaggag agacgcatgc ccttgagggg tttttccttg gcaaagcgct cgcggatcga
    29341 acggagaacg ggcatttcgc gttccgccca ttcgatacgg cggcgtccac cttcagcctg
    29401 ttgaatatct ttaatatcgt aattgctcat gtattgctcc ttctaatttt ggtaattggc
    29461 aacttgtaat tggagattgg cggaattacc aactaccaat tacttcaata atgcatccaa
    29521 ccttccagaa tcatcaaagt gatcgcggaa acgctggata aattcctgtt tagtataact
    29581 gtgattctgg gtaccgcgct gttccaggca gtacactgcc gaaagcgaac cgatctcgcc
    29641 gcacaacttc caatcgaaac cgttggcgta gcctgcgagg aatcctccgc ggaaggcatc
    29701 gcctacgcct gtgggatcca cgatctcatc ctcagggaca gttgggatgt gaaccgattc
    29761 accgccagcg tacaagtcag cgccatcttt gccgcgggta atgaccagca ccttgacgtg
    29821 tccgagaatc tggtcgaggc tccagcccgt cttctttgaa atcagaccga attcatagtc
    29881 gttacagaag aggaaatgcg cgccttccat atcacgggca agttcatgcc cttcgaggcg
    29941 taacacctgc tggctgggat catacaaata agggatgccc agctcgcggc actcggcagg
    30001 gaatttcatc attgcatcgg gtgcgctggg agaaacggtg acgagatcgg gtttcttcgg
    30061 caattccttg atggattggg tggcggagtg ccccatcgct ccggggtaga acgatgcaat
    30121 ttgcgccgaa gcctgatcgg tcgtggcaaa gaacgaggcg gtgaacagac cgggaaccac
    30181 tttcatcagt tccgtatcca caccttttga gtcgagccag gcacggtagt cgccaaagtc
    30241 ctcgccgacg gttgccatca cgcgcggttt ttcaccaagc aacgtcatgg tgtatgcaat
    30301 gttcggcgca atgccgccac gctgcttcga catggattca acgaggaacg ataaactgat
    30361 cgaggcgagg cgttcgggga gaatctgttc cttgaacaga ccggggaagg tcatcaaata
    30421 atcgtaggca acagaaccag tgagaagaat gtccattgat ttactcgcag aaaaaggcgc
    30481 actcttgcga gtgcgctgga tttacttaca aacgtgagtt tatcacggga taatgtgatt
    30541 gtcaaagaaa aggatatgta tcacaccgcc gctgttctta tccatttcac gattacggaa
    30601 tggcacgaat ggagaagtaa tcaaagtctg cgggcttctc ttcttcataa gcctggctcg
    30661 cgatcagccc gacaaacgca ggtgtgatat cgcttgtgtg ctggccgatt tgggtccaat
    30721 ttgaggcatc ttcactgtaa taggccgtat aggtattgcc ttcacggagc aatcgaagat
    30781 agacctctga ggggctggtt atgggtgtgg caaaattcgg gccgttttgc tgagttgggt
    30841 tgtcgaagta caacgcattg cctttgcagg gagggaaatt acactgggcg aatgcccgtc
    30901 cgaaaccaag agcttgcccc tggccttcgt agatcaacag gcctgcaaac tgaaaattgg
    30961 tcgtcggctc gaattgcaca taggtttcaa tttcaaaatc cccagcaggt gcggcatgga
    31021 caagaaaatt gatcggttcg gcatcactcc cgatgtttgt tccctgggtg atgattcgca
    31081 taaagccagg cacttcggcc aagctccagt gtgtggtgtc ttcccccaac cacacccaac
    31141 catcagcaag ttgaccgtca aaatcatcac ggaacagaaa gggtggcttg gctgtgggcg
    31201 caggtgtttc tgtgggcagt ggtgtattgg tcggcaccgg ggtattcgta ggaggcgatg
    31261 tcggcgtcca cgccgatgcc gtcatcgtgg atatttgttc cgcagacggt ccacacccac
    31321 ccagcaaaac aagcagacaa aatcctgcca acatggaaaa tacacgtttc atttttcact
    31381 cctttcatca agtggaatca aaacgaacgg caacgtcagg cagtttataa aattgatcgt
    31441 tcacaaaaag aagatttgaa ccgttaaata ttttcccgac ccatccccgt ttgcaaaatt
    31501 cataaccacc ccatacccac cttcaatttt attatacgga aattatcctc aagattcaat
    31561 cactttgcaa gaggtcaccc tgtcgtgcag ggtgacctct tgggcttcaa acctaactta
    31621 tttcacctac agtttattca agccaccctg caaggcttga taactttgga acgccagaaa
    31681 cccaaacaat accgccatgt aagtgctgcc cagcaaaaag aaagcaatgg cagcaatgac
    31741 aatccctgcg atgaacgaaa gccacaatga cttacgcaag ccgtcccagg gatcagcgat
    31801 gagcagaatg tatctggtca cttgcccgcc atcaagcgga tagacaggta caagattgaa
    31861 cacaccccaa aagatattga cccagagcag gtctgtcagg aacaggtcta aaaggtacaa
    31921 attgaacggc agggaggcat gcacaggaac tggaatcaca aaaaggatcg tacccattac
    31981 gatggagcca cccatgataa aggtcaaaac agagaccagc agcgccaacc caaaccctgc
    32041 aaacggacct gccaacgaaa caaaaatttc ctgatttgga gtgagtgcca cactggcgat
    32101 cccccctccc caccgcattg actcgggtac ggtcaacccg cctgaagcat gcaggagaat
    32161 gttcgaagag atgccatggc ggcgcagggc aaaagcatgc cccaattcat ggacgatgat
    32221 cgaaatgaag acgaccagca cccagatcac aagggaaatg atcccgccag aggtggaccc
    32281 cagcagaacc gcgatcagcc aaaacaaagg ctggacgcga atcgggatcc cagcgatgga
    32341 aaatctcagg tcaaaacggg tgggagggga atatggaaag agcatttatt tcacaacctt
    32401 tttgagattc atatcaaacg gcggatacac cacaccattt tcagtgacga tgccgctgag
    32461 caggcgattc ggtgtgacat cgaaagcggg gttgcgggct ttggcatttt tcggtgcaac
    32521 aggttcacct tgaaattgaa tgccaagaac ctcagcggta ttgcgttctt cgatgggaat
    32581 caagccgccg tgtgcgagag acaggtcaac ggtggaggtg ggcacgaccg cataagcagg
    32641 gacgttgtta tcgtttgcag ccagagagag catgtaggtg ccgatcttgt tggctacatc
    32701 accattggcg gccacgcggt cagcgccaaa aaatactttt tgcgccttgc cagccttgag
    32761 aaaatacccc gacatgttat cgctgatgat ctcgtaggga atgccgtatt gttccaattc
    32821 ccaggcggta agacgggcac cctgcaagcg cggacgggtt tcatcaacga ggacgtgaat
    32881 ccgttttccc tgttcgtgcg cggtgcggat cacgcccaat gcggtgcccc aatctacagt
    32941 ggcgagcgca cccgtgttgc agtgatggat gatggtgtcg ccatcgttga tgagggctgc
    33001 gccgtgctcc gccatgcgct tgttgatttc cacatcttca tcggcgaggt tttgcgcttc
    33061 ggtcaaaaca gccgcacgca cggcgtctgc gtttccttcg tctttcattt gttccgccac
    33121 acgcatcaaa cggtctacgg cccacgcaag gttgaccgcg gttggacgcg aagccttcaa
    33181 aacggcggca gatgcccgta ggtcagcgag caggccttcg gttgaggtgg atgcggattc
    33241 atatccagca agagccaggc cgaaggcggc ggcggctcca atcgcaggcg cgccacgcac
    33301 caccatatcg gtgatggcat gcgccacttc cttgtgcccg cgataggaga gaatttcaaa
    33361 acgcgcaggc agaatgcgct ggtcgatcat tttcaattcg ttgttttccc aaaaaactgt
    33421 acgcatggtt tatcctttat cgcttgaaac gatgctatga aaaatatttt tcgtgcaatt
    33481 cgatcaattt cgcctgtgcc tcgggcggaa cacgtttcac ttcgcccagc atttttgcgc
    33541 gccagtaaat ttcagagacg tgttcaatgc gttcgagatg aatcagagcc tcatcgagat
    33601 gtttgccgta ggtgaggctg ccatgttgac aaagcagcat ggcattgtga gttttgacaa
    33661 aaggacgaat gacctcggca tcttcgtgtg aagagggggt ggcataggcc gtaatgggca
    33721 catcgccaag tgtgagcagc acctcgggca atacatccac agggaattga aggcctgaca
    33781 cggtaagcgt ggtcgcatag atgggatgcg catgcagcac accgcgcaca tcgggccgct
    33841 gtttgtacac ctcaaggtgc atgggcgttt cagaagaagg tttgcggtcg gggcgggagg
    33901 aaataacttt gccttccaga tcaaccacaa gcatatcgtc ggggttcaag cggccttttg
    33961 aaataccaga aggtgtgatg agaatattcc cgtccagcat gcgcgcggaa atgttcccat
    34021 cgttggaagt gaccagatgc cgctcatagg caatgcgccc gcattcaatg atggcgaggc
    34081 gtaattccgc ctcgctggca gtgggagaaa aattcagcat gatttcaggc tactccttga
    34141 tttccgtcca gatcttgtcg tacagtgaaa gcgcattgcc aaggtcttcc actttatgtc
    34201 cctgcgcgaa aatttcagcg ggggtattgg taatgggtga agccatgtag gcttcgtaca
    34261 cctctgcatg attcgctttt gcgaaatcga gcgcggcttg attgggaatg gtgtagggat
    34321 aatccaccag tgtcagccaa aagacatcgc cctgcatcat gtaattaaac caggcgtagc
    34381 tgatatcggg gtgcggggca ttggtgggaa tgcccatacc atcgaagaag atgatcgaac
    34441 cttccttggg aaaggcatat tcaaaagcgg cattttcctg ccttgccaaa aaggcttcgc
    34501 cattccaaac gatgccaagg tccacatcgc cagcaacaag cggagttttg ggactgtcgc
    34561 tatcaaaagt gcggatgttc ggcatgagtt cgataagttt ttgttttgct tcttcaagtt
    34621 gggcttcatc ggtggaattg acatcgtacc ccaatgtaag cagagccatg ccgatcacca
    34681 cacggttatc atccacggca acgatgcggc cttcgtactc agggctccac aaatctgccc
    34741 aggaggtcgg cggggtcttg acggtttcgc tgttgtacac gatggcttgt gttcccattt
    34801 gatacggcac aaggaaatca agcgaattac catacgcgct gatcaaattg ccatccaggt
    34861 ttttcgtatt tgcaagtttg cctgcatcaa gtttctgcag caaaccttca cggatcagca
    34921 catcgatcat gtaatcgctg gggtggacca catcataggg attcacgctt tcgccaaaag
    34981 acatcttcgt ataaagttct tcgttcgagg aaaaataatc cacattcaca tccacgccgt
    35041 agaccaggcc gaagcaatcg atcatgtcgg caggcacata ttctgtccat gtaaaaatat
    35101 tgatctcttt gctttcaaat tccacgcgcg ggctgggttc ggggcagacg aatccactgg
    35161 aggttacgtg ttcggtggat tcctgcggcc tggctccaca tgcggcaatg ctcaacaaca
    35221 taaacgcaga caaaatcaac cgtccaaaat gataaatttt gtggtgtatg ttcattttct
    35281 tttctttttt tcctcttttt attttgatgt gccacaaatc atggcagcgg ttaatatatc
    35341 acacacaaac cccgaaaccc cgcttgacgc acctgtcttt taccagtata aaaacggaat
    35401 gaatttcacc cgaccatcca caagacgaat cgactctgac acgcgacgca gccgcctgcg
    35461 cacaggtctc cgcttctttc ttggactttg gcttgccacg acgataacgg catgttccat
    35521 ctttccgaat tttcccaccc tgcccccagg ctggaccctg accccaagca ccacccccac
    35581 accagaagcg acctccaccc ccaccatcac ccccactccg ctgcccatcg cccgtgtgga
    35641 caagggcgac cacgccctgt tcaatggaga ttacgaaagc gcgatgctcc attatcaaac
    35701 tgcgttgagc gattcgcccg atccgctggt gcaggcggcg gcaaaatggg gggaggcgcg
    35761 tgtgcgtttt gcagaagagc gctacgaaga aaccctgctt tccacacagg aattgatctc
    35821 acaatttccc gaatctccca acctcggtca cgcttatttt ctgcagggat tggcaaatta
    35881 ccgtttggga aattatcaag cggctgcaga ttcgtggcag acctatctcg ttgtgcggcc
    35941 tggtcacctg gatgcacatg tgcaggaatt gcgcggcgat gccctgtttg aagcacggga
    36001 tttccccagt gcgctttcgg cttacactgc cgccattcaa gcctctgcac ttggcgatga
    36061 cactcttctc gatctaaaag ttgcttcaac ccaggttgcc atcggcgatt acaactccgc
    36121 gctggccttg tatgatggag ttttcgcacg cgcatccaat gattacatca aagctcaaac
    36181 ggcgtacgag tcagggttgg cgtatcaggc catcggtcag aacgaagagg cgctgggacg
    36241 ttttcgtctg gcagtggagc aatatccgct ttcgtattat tcgtatctga gtctggttgc
    36301 gctgttggag gcaggcggca cggtcagtca acttgaccgc ggtttggtgg attattttgc
    36361 agggcaatat gatgtggctg tggcggcctt caatcagtat cttgttgaga atccaaccaa
    36421 tgatgggacg gcctattatt accgcgcctt gtcgcaccgc gatcttggca attacgatgc
    36481 cgccctgcag gattacaaga aattcatcga agggtatccc acccatccgc gctgggtgga
    36541 tgcgtggggc gaaaaggctt ttattgagtg gtacaacctt ggctcttata ctgtgggcat
    36601 caaaactctt ttggattttg ttgcggctgt ccctggcagc gaaacttcag cagattattt
    36661 gatgagtgca gcacgcattt acgagcgcaa cggcaagtat gaagaagcca ttcaaatctg
    36721 ggcgcgggtt gcaaatgaat acccaggttc agagcaggcc tctacggcca ctttcctgac
    36781 ggggatcatc cattaccgca atgggaacac cgtccctgcg ttggatgcgt tcaatcgcag
    36841 cctcaatctc tctgtgctgg cggtggacca atcacgtgcg tatctgtggc ttggcaaggc
    36901 ccaacaaaaa cttggtgatg gcgaagcagc ggtcaatgcg tggcgagcag cgcaagaccg
    36961 tgaccctggc ggttattaca gcgaacgcgc ccgcgacctt ttgctcgaaa gacaaccctt
    37021 tcaagccccg atttcggtca acctgacacc caatctcgaa tctgaacgca aggatgccga
    37081 ttcttgggtg cggctcactt tcaatcttcc gcttgaaaca gacctgagcg gattggggtc
    37141 attggccagc gatccacggg ccattcgcgg caccgaattc atggaacttg gaatgtacga
    37201 agacgcccgc cttgaattcg aaaacctgcg cgccgaactg gaaacaaaca tggacgccgt
    37261 gggaagttat cggctcatga actatctggt ggatattggt ttgtatcgct cggcgatctt
    37321 tgccgcacgg caggtgctta ccatcgcagg gctggatgaa cacaccgaat caatgatggc
    37381 tcctccctat ttcagccatg tgcgctatgg gttgtactat tcagaccttg tactccccga
    37441 tgcagattca aaccgctttg acccgctgtt tgtttttagc gtgattcggc aggaaagtct
    37501 gttcgagggg tttgtcaaat ccagcgcagg tgcgcgcgga ttgatgcagg tcattccctc
    37561 cactggtgcg cagatcgttt cgcagcttgg ctggcctgtc aactacacag atgacgacct
    37621 gtaccgcccc gatgtgagca ttgcatttgg aacacactac ctcggaaaca atcgtgacct
    37681 gctgggcggc gacctgtatg cagcactggc agcatacaac ggcggccctg gcaacgcgat
    37741 cgaatggcgt gaccttgggc agggtgaccc cgacttgttc cttgaatccg tccgctttga
    37801 ggaaacgcgc aattacatcc gcaatattta cgagatttac accatctatc ggcgcttgta
    37861 cgggctttca gaataaggca ataaggcatt aactcaaaga cgcgaagacc gcagagaatt
    37921 ctggcggctt cgcgtcttta tatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38041 nnntatattt caaaaatttt ttaccgcgaa gggcggtctt cgcgaacttc gcggttcaag
    38101 ttgtcatttg catggccgcg aagaaaaaac aaaaaggttt attccatgtg tgaaagaatg
    38161 cgttgatgcg cggtctcaac gtccatatcc aaaatcgaga ccaatttgtc gcggccattt
    38221 tcgccagcaa cgacttcaac gcgcgtcttg ggcactccca acacctcgga aagataccca
    38281 accaattcca cattcaaaac atcgtcagcg ggttcagagg caaggtgtac cttcacagtg
    38341 ccatcgctca acacacccac aatctggttg cggctggcac gcggtgtaat ccgcactgcc
    38401 aaagcagacc cacgttgacc gctatgtaaa acgaattttc tagtcatggt tcacctcaac
    38461 tcaaagaaat taggaaggca atcaggatac gagacaaaac atcaatggca agaatcaaaa
    38521 ccacggggct gaaatccatg gggccagcag caggcagaat acggcggatc gggtcaagaa
    38581 acgggcttac aatgcgctca agcgcctcac gcacagggtg atacggggga acaaaaaaag
    38641 aaagcaatga ataagcgatc acgacccaaa tgaaaagagt ggaaagattc cttatcaaca
    38701 aaatcaaaat ctgaaaatcc atcaaacctc cgtcttatat tgtcgcggac caacgatggc
    38761 agtgccgact ctcaccaggg tagcgccttc ctgtacggca acttcgtaat cggaactggt
    38821 ccccattgaa agctccgaaa aatcggattt gggaagttgc gtggaaaggt aagcctgcag
    38881 ccgcttcaat ttttgaaaaa acggacggga gaattcggca gtttcgccaa gcggcggcat
    38941 ggtcatgagt ccatgtactt tcaagttggg cagagacatc accacaccaa gttcatcccg
    39001 cagggcaggc cattgcgctt cgtcccatgc agaccagccc gatttactct cttcgccgcc
    39061 cacgttaaac tccagtaaca caggcagcac acgccctgcc tcgctgcaaa aacggtcaag
    39121 tcggttggct aatttcaggc tatcaagcga atgtaggtag ttaaagttcg cagccacgag
    39181 ttgagccttt cggctttgca cgtgaccgat catatgccat tctaccgcag aaaactgacc
    39241 caaatattgt attttcttta cactttcttc ggggtaattc tctcccagca cctgcgcgcc
    39301 cgcttcgatc acagcgcgca ccacttccac aggctgggcc ttcgtcacca ccaccaggcg
    39361 gaccgaatca gggttacggc cagcactgcg ggcggcggat gcaattttat ccagcgttgc
    39421 cagatatctt tctcggatgg atttgaccag atcgctcatg ccttaatttt accccgcatc
    39481 tttcctaacg ttaggcctgc aacgccatta atgcgccgaa tctacccgtt ttacctttct
    39541 ctgcccgatg cgaaaaccag tcatccaaat gacaggcggt gcaaacctct gagacctgga
    39601 tctgctccac gcctgcctgc tgcaactgca tcgagttcga cttccacaga tcgaggtaaa
    39661 tacggccttc cctgaattcg aacatggcat cagcatggct gttgaatttc tcacgaaact
    39721 gcgcgagaat ttcagcgcca acctcgtaat ggtccacgcc gatggatggg ccaatgccag
    39781 ccacaatgtc cttgggatca ctgccatagt gagagcgcat cccatccacg gcggcaccca
    39841 ccacaccacg cagggttccc atccaccctg catgggcaat gccgatgact tttttcttcg
    39901 ggtcatggaa aaggatggga acacaatcgc caaagcgcat gaacaatgaa accctgggat
    39961 tgtcggtgaa aatgatatcg gccttcgcgg aaggctcatc aagcggacga ggtgcatccg
    40021 cgtagacaat gtctgttccg tggaccagcc acacgtcgtg gatggattcg ggttcacatc
    40081 ccatggcttg aaaaacacgg atgcggttcg cggcgacgtg cgcagggtcg tcacctacgg
    40141 atcccccaag gttaagcgaa tgccacggcg cagggctgac cccgccacgg cgggtaaaga
    40201 cagcctgtgt tacagcgttt gaaaaaatat caaaacggta ataccgaaga ccatcgtgtt
    40261 cttgaaaggg cattattgcg aagcctcgtt gaccgcgaat ttcttgatgt aatattggcg
    40321 ggtttctgcc atggattctt caatgttggg gtaggcaaac catgcagtgg caaggccaaa
    40381 gagggcgccc gtgagtgcgc gcaggaatgg ggtgctttcg cggtagggaa cgacagtggc
    40441 aagccaggtc cagttgaatt ggctgaaaag ctgcgagaag ccgtcaaagc caatgggcgc
    40501 aatgccgatg agcaaccaca acacccaatg cagtgatttg atgcggcggc ccgtaagacc
    40561 ataggtgatt ccgaaaagaa ggatggcaag ataaatggcg acatcgcgtt cacacaaggc
    40621 cactttatag ccaaccgtct catcgccgat ataggcacgc gcttcaaaac gggtgaagct
    40681 gtagggatcg tcgagatttg gaatgcctgt cacttcttcg aaagtcttta cccccgccac
    40741 attcgcctct gccagcggat aaaacgcctg ctcaccatac aggaagaaag acctgaaccc
    40801 aaattgatgg cagagcgggc tgtagattcg ataaatgacc ctggctggga gttctgcgcc
    40861 caacttcatc aatgttggtg caaggagcgg caggccaaca taaaacaaca taaacagatt
    40921 caaaagcaac agataacgtt tggcgatcca aaacgaaaca cggtctccag tggtcacggt
    40981 ctttcctttc ttgacgcgga tttgataatc agggtcgccg acctgttcca attggttttt
    41041 cctgtctgca gcggcgccga gggtcatttg caaattctgt cgtgaaatgg gagccttcaa
    41101 tgtgtaagga ccaacttcca ccacgggaat ttcgatgccg tatttatcga tcaatgcggt
    41161 atcagattcg atatccactt ccacaaggcg gtgtggatat tttgattgca gttcctgcaa
    41221 ttcgtgtttt acttcgtcgc acaatttgca attcttgcgg gtgtagaggg ttacattaag
    41281 catgggaaag gcctctcatt atcgcttgtt tcatcatcaa acccgcacgg atgtgaagga
    41341 tccgcgcggg tttgatgatg ctttagatct gtcaaagggg gatggaatta caaaccaaaa
    41401 tcaatataga agccttgacc ctgcaacgca agggtttcaa acacacctgt caaaagcatg
    41461 acgccgacca caagcaaaat cacacccatg acaatttcca cataacgcat ggtcttggcg
    41521 tagttcctca aaatgttggt tacccagcca atgcccaaag ccgcgaacag gaatggaatt
    41581 ccgaggccga tggaataaga ggtcagcaaa actgcgcctt gtgaaatcga gccgccattg
    41641 aaaaccacgg tgagaatggc tcccagcaca ggccccacac atggagacca acccgccgag
    41701 aaaaatacac ccatcagcgc cgaggaaaga taacccatct tcgggtctgg tgcctgttga
    41761 acacgggtgt catacgccag gaagggaata tgaaaaacgc cgatcatgtg caggccaaag
    41821 gcgatcacaa caacgccgcc gatcttggtc agccagaaac gcaggtcaaa cagcacccca
    41881 cctgcaaaag ccgcagccac accaagaaaa acgaagacaa aactaaatcc caacacaaaa
    41941 gccagcccgt gactgaatgt gatccagcgg tttttgctat tgctttcacc acctgcggca
    42001 cgcccgccca gataccctac gtaagcaggg accagcgaaa acacacacgg agaaaggaag
    42061 gaagccagcc cggcaagaaa ggcaagccca acactgattt gagaaaattc catttgatta
    42121 cctcataaat taaatccttc gcccttcgct caggacgaag gatcttgatt acaacttcaa
    42181 ttccttggtg gtgaccacgg tgccgtgatc gggccaggtc atgcgggttt cacattgtgc
    42241 cagcgtaatg taaggcgttg tccaacggaa gatccacttg gcatcttcgg gtgtcacgtt
    42301 gatacatcca tgcgaacgcc tttcaccaaa atcgttgtgc cagaacgcac cgtgaatggc
    42361 aattccatcg ccatgaatta cagtgctcca cggcacggcg ggtgtggaat accccgaagc
    42421 cgaaccgccg ccgctcatat tcagtgaaat gattttccaa tgggtcaaca actcacccac
    42481 tggggtcgcc cgttcttcag aaagcgcgcc tgtggcatcg aattttgcac cgctggaaat
    42541 gcggcagaag aaaacctcgt tattgccttc ataacaggaa agggtctggt aggtgagatc
    42601 tgcaaggatc tttttctcat tggggtcgat ctccgggctg atcggcgcca catccacatc
    42661 tgtgagcact ttcaaacccg cgccatccag ccagtaaata tcgccaaacc catacccatg
    42721 cccaggcgat tcattccacc gatattccgc aaagccattc gtagtgcgaa cctgatcgat
    42781 ccacaacacc tgcccataat agacacgcgg cggaaagttg tattggatca ggctttggat
    42841 ccacggcgag atgggggctg tttcgagggt caagtcaaca tatgggacgg ttacttccgc
    42901 ccaaaagcca ggctgacccg ctggcatttc agtgatcggg gtatttggta aattcctcgt
    42961 cggttgcacg cgtgaggccc acacatatcc ctgcggggtc tcaagatact tttggttggt
    43021 caggccgatc acactgccaa tcacctcacg tccccacggc atcaaataat cggcgggcat
    43081 tgctccgatc acattgttca agatcgggtt gttatccggg cggctgcgta cgtcggtctg
    43141 atccacaagg cggccgataa tgtcgcccgc agggaattga ggcaggcgtt tcggggtata
    43201 aagtttttcg taataggttt cagaaaagta tggcttgaat gccagagcac ccaacccaac
    43261 acctgcaact tttagaaaat cacgacggga aaaattttgc ttcatacttt acctttgtat
    43321 aaaaatgaat cgcggacttt ggttcgcatt cagcaaggat acctgcaaac aaaatcagcg
    43381 tcaacagcct tgtgagaaat ccttaattat tcaagtaatt ttccaacgca gaaaggcaat
    43441 ttgcgcttaa cttatgcccc acttcatctt cacaataggt cacctgcgcg cctgcctgtt
    43501 caagcaattc aattgaagca cgggcacggt caacggggat catttgatcc tgcgtgccgt
    43561 gtgccacaaa aatatttctg cctgccagca ctttttgtgc gatctgttcg tccaaccctg
    43621 aaggcatgaa tcctgccaac acacccatct tgcggacacg ctgcggatag agcaagccga
    43681 ccacattggt cattgccgcc ccctgactga atcccatcaa atcgaattgc gacgcatcca
    43741 acttaaccga atctgcatac tcatcaatga ggtgaatcag cgcctcagcc gctggttgca
    43801 gcatttcgag gctgggcctg ccaaaggtcc cgccttgaga cgggcgccag gagaatccgc
    43861 ttggctcggt ggggtgtgga gcgcgtggcg ccatcaaatg gtagtctgtc gaaatcctgc
    43921 gggcaaatac ccacattgaa ttttcatcgc cagtaaaacc atgcagcatc acaagcagcc
    43981 gcggagaatc agaattcgat tcgcggactc gcaaggtcca gtcgttaaac gtgatcaagg
    44041 tcgaattatt tgcgtccggc attcttcatg atccaatctg ccgcgtaaaa gattccgacg
    44101 atcagcacca caggaaccag cccgaccatc aggcagaaaa atcccattga agctgcacca
    44161 ccgccataga tcaaccatat caggccaacc cccacgacaa gcaataaaac cattgcgcca
    44221 ataataaggc ggcggttggt ttgcttttca tattccttta aatctctact cattgtatcg
    44281 ctccaaaaat tcttctgttt actcttgctg gccatgaaac cgcacgcttc aattgtggca
    44341 gaacctcttc agtggcccgt tcaccaagtt tgatgacatc ggataccacc actttatcca
    44401 gcaggttgat atcatgtaca ttcgggcgga taagcacatc gggcttatcc aactcaagac
    44461 gaaaatgcgc caccgcacgg ctgcttacat ccacagcacg cagaaagata tcgagcgact
    44521 gggcgaaatg catgcgtgtg atccgttcgg cgatggagcg agggaggatc tgcggcatgg
    44581 gaatggtgta ggcaggtaca ggtttatcca tgggatcgtt gagcatcact gccaccacag
    44641 gcagtttcgg tgaaagcatc cgcgctactg aaacaggcac aggattcaaa acaccgccat
    44701 ccaccaattc ccaatcgttt atgcgttgtg cgggaaaaat accaggcagg gcaatcgtgg
    44761 cgaggatcgc atctttcaac ggcccctcag aaatcgtaac ttcacaccca gatttgacat
    44821 ccaccgcagt aaccgcacat ggaatgcgca gatctttgaa ggtacgctga ccaaccacct
    44881 cgtccaataa tttccgaaca cctgccaggc caagcaaaga tggcccatcc tcagggtggc
    44941 gtccatacaa acggctttga tctactgttt caaaaatttc ctgaatgcca tctggtgagt
    45001 atccaatggc gtacaaaact gcgacgagac caccaaaact ggtccctgcc acagagcgga
    45061 tcaaaaatcc ttctttaaga agacggcgca atacgccaag atgtgaattg cctttggccc
    45121 cgccaccgcc taatgccagt gttatttcca tagttttctt ttcgtagttt tgtattttgg
    45181 cagtctatta gactgcattg cgaagagcaa ttataccttt tgccagttta cagtcatttg
    45241 atacctatta ctgggatctt acaaaaacaa acaggccgca ctcgaagcac agcctgcaca
    45301 aaacatggat cattgcccac tttaattcgg gtcgcgaatc aaccagaccg ccaccccacc
    45361 accgacaaag gcagccacaa ccaagatccc caccagcaaa ccagaagaat tactcgatga
    45421 cccttcttcc tccacaggca tatctgttgc gggtacggtt accacctgag gaattgatgt
    45481 tggtgtgaca ggtggaatgg gcatttcagt tgttatcaat tccgcagaag tgggagtttc
    45541 cataacttga accatggtcg gtgtagctgg aggcggggtc actcctttta ggaccaatag
    45601 ttcctgtccc tgatatacca ccaagtcttc tccgaggccg tttaatgcct gaatcgcttt
    45661 gatcgtcgtt ccatattcaa tggcaatgct ccacaaagtt tgcccgtatt gcactttatg
    45721 aaacacatct ccattggcat ttgccgtact caacatcaca ggaaccatat attggctgac
    45781 tccagcttgt gccccaccag gcacactggt tagcaatgta gtgacaccat cctgcatttc
    45841 cccactaccg gtggcagctg cacaatctaa cgcataaaaa tacccacttg ggccctgcga
    45901 gattcctgct ccaatatgag taaaattctg cgaaagcatg gtgttcatgt gttgactatc
    45961 ctgccaggca ggcggcacac cattccatac caacggattg gaagattgga tcacattctc
    46021 agcacgaaaa ccgccgagtg acaggtcacc ggcaagcggg aaccccaaag aaagcaattg
    46081 ttgggtaaag ctaatcccac ctggacgctc atgggtggca tacccaactg aagccatata
    46141 atctgcctga gattgggaag actgcattaa caccgaatgg acggccagtg caggcaatcc
    46201 atttgctata cgcaaattat ttacagcatc tatcaactga gatggtgtgt taatggtctg
    46261 gcgaggcagg gactcagcct tactcggggt tggtaacagt acagcacaaa ccagcacaga
    46321 aaacaaaaga aaggaaaacc aacgagatag gtttcgcgat cttgattttt tcaagtttag
    46381 aatctccact atcatgggcg gtataagtca atgcatgtat tgttttacat gcaaaactaa
    46441 accgccactt gaggcaacaa aatctgtaaa gccttgacca gtttattcaa atcttcctga
    46501 gaattgtacc cctgtacaga aatacgaaca aactgcctgt tttgccattg tatcaatggc
    46561 acttccacct tgtattcatc atacaaacgg ctcttaagtg tggcaaggtt ggattgcgga
    46621 agcggagcaa tgcccatttg gttataaaaa tcagagtcga gcggatatag agactccatt
    46681 ttcaccattt cacaaatttg ctcaagagtt tgccgcaaca aatcatggct ctcttttcgc
    46741 acctcatccc aattgttttc ctccataaat tgaatggcgc taggaacggt caaagccgcg
    46801 gctgggtctt tggtacctgt ccattgcaga atatcaataa aacgagatcc tgtggttgtt
    46861 tctggagtcg cgttgtatcc ccagctcacg atcaaaggat caatcagatg ctgcagctcc
    46921 cttctaacat ataaaaatgc cgatcccttt gcgcccaaca tccatttatg gcagtttcca
    46981 aacacaaaat ctgcatcaac tttctgaagg tcaacatgaa tttgcccggg ggaatgagcg
    47041 gcatcaataa ctgtcaatat acccgccgcc tttgcccgac gggagatctt ctccacaggc
    47101 atgcgtaaag ccgttggaga agtaatatgg cttaaataaa tgacttttgt ccgcggagtg
    47161 actccctgcc aaagctgatc gacaatctcg tcctcaatat aagctggcag tggaatgggt
    47221 tgatgaatgt atttggcccc aagttttcta caaacaaagt cccaggtata gtcacaggca
    47281 ccatactcat ggtcagatgt gaggatttcg tctccgggtt caagatgcag ggagcgggca
    47341 acaacattta ccccatgcgt tgcattaggc acgtaaatta aatcctgtgg atcagcgttt
    47401 aagtagacac ctaatttcgc ccgcgcatca aacaacaact ggtcatattc tcttccaagg
    47461 aaaagaacag gctgttgttc aagtcgtaat tgccaatttt gataatcctc aaaaacaggt
    47521 tttggggtgg ccccaaaaga gccatgattg agaaatgtaa ctgaaggatc aaggtaaaag
    47581 tattttttta gattttccat tagcaaatta taaattaact caggcacatt aaaaaacaaa
    47641 ccatttttta acagcctaaa ccacaaac
//
LOCUS       DAOQJC010000009        47424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_309_length_47424_cov_9.822099, whole genome shotgun sequence.
ACCESSION   DAOQJC010000009 DAOQJC010000000
VERSION     DAOQJC010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 47424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47424
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_309_length_47424_cov_9.822099"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1794)
                     /locus_tag="PLE39_02305"
     CDS             complement(<1..1794)
                     /locus_tag="PLE39_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE67182.1"
                     /translation="MNSLKTKLTIYQGLVGFAGILLIVSITWLVSRQRILSNAIAEAE
                     NLSDQIAYSMVVIAAQGGDATFNYQRLIEKTSTLNNVSSIKVLDEKGVILADNDHSQV
                     GQVFNSPLLEDSLKNQRKEQQIEGRSLIVVRPLRGETYTANLNDVTGFLWIELDFTPA
                     YARAQEDMSLILAVSLGGFLLTFYWYYRVTQNGILDRLSVLSSGLIKVENGDLTQRIS
                     IRKLFGSTDEINELASQFNRMTASLHQKLTFEELTSHLSAKFMNVPFSEMDTAVHDTL
                     RRLGELFKVDRTYIFEFTDDGLAMNNTFEWCANEIIPQIDNLQGVPREIVPWWMQLLE
                     NGEIIIIPRVSEMPKEAASERQILSEQGIQSVLVTPMTSDHGLFGFIGFDSVAHERNW
                     TKEEVNLLSMLSGVVANTLIRQRSQREVVEERDFALQVMETVAHGLIVADLDGSLEYT
                     NPAYAKMLKYTAQEITGRKIQEFMHIEDIPQDETQNSYTAQMRLIAKDGTVVNTLVSR
                     SPRIKDGHIKGSIGAVVDLTEQLLAEEKLRQSEARNRAFLNAIPDLIFRMDEAGRFID
                     FKAGDDSRFYIPPEEIIGASVADILPAEVSNM"
     gene            complement(1816..2808)
                     /locus_tag="PLE39_02310"
     CDS             complement(1816..2808)
                     /locus_tag="PLE39_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE67183.1"
                     /translation="MHTNKTFRWAVILLLLASIGLSGCASQKKPIRIAISAWAGVEPA
                     ELAAQLGYYEKYGVDVELVRFSAYSDSLEALRDENVDGGMHTLDDSLRNMAAGRDVRV
                     VLLTDYSFGGDGLVARAGIENLSDLRGTKIGVEIGTVGHLSALKILEKAGVAVSDVTF
                     ISIPAWEIQQAMIDGQIDAGVTWEPYLTSTAEMIGGKVLITSREYPETIVTTMTFNAK
                     LIDARPDDVQKIVAAYFDAVEYIKQNPQEAYTIMGKAEGISAEEFSTHVMGIRYLDLS
                     SNAELFGVNSDGRVYAAAQEISVFLADNNVITTMPDVQQMLEPTFVLTLFKDGQ"
     gene            complement(2824..3942)
                     /locus_tag="PLE39_02315"
     CDS             complement(2824..3942)
                     /locus_tag="PLE39_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNE67184.1"
                     /translation="MSDSRKTILVIDDEFHLLVGVKALLEHEGYNTVSTSDGMAGVQM
                     ARKVQPNLIICDVMMPFMDGFKVRETLSEDPVTKDIPFLFLSARASQTDKLRGLNNGA
                     DDYITKPFDHRELLARISAVFNRYEKGQGDMRRKMDEEILRFEREISHNISHELRTPM
                     TQILLALDMALRGKYENIDDLKLFVETALSQSHKLNGLIDDLIFLNNYDRGMVNTLRQ
                     EVDLKADFMEPIKFLQDLYAEKNIKTQVKIDPTVTIHAPRREFKRAIGHLVDNAFKFS
                     NPMTTILIDLEENEVGGFVLTVTDYGHGFAQQYSEKVFNRFFQIDQGDCRAYNGLGVG
                     LTVARIFARSLGGDVTIFPSTRGCRVQMMVPPGSTEIP"
     gene            complement(3961..4986)
                     /locus_tag="PLE39_02320"
     CDS             complement(3961..4986)
                     /locus_tag="PLE39_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE67185.1"
                     /translation="MENKTSLLLIDDDPALLVGLEAVLRYEGYRVFTARHGRTGIDIA
                     RESTPDLIICDLMMPPPSGMEVLTILGQDPRTANIPFIFLTARSSSTDKVHGLLSGAD
                     DYVIKPFVKDELLARVKTILRRKQKAPVLSSATDKDEIQHLRNEITEILRNSEVNWEK
                     FVDSLVHMLGLRNNETEDHAWRVMNLTEKTAIELGIHGVELIHIRWGAILHDIGKVGI
                     PDNILLKPGELTKEERNVMMMHPQIASQILEPLGLPSTTLDIPLYHHERWDGKGYPDH
                     LAGDNIPLSARIFSVVDVWDALTNDRPYRKAWSCEKAGDYILGEAGKSFDPHVVDVFL
                     KKVISYS"
     gene            complement(5416..5916)
                     /locus_tag="PLE39_02325"
     CDS             complement(5416..5916)
                     /locus_tag="PLE39_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67186.1"
                     /translation="MRYGSMYRMLFVLLCFSMLSLACNAVTEAMSTPPPVVPTVFTGG
                     QESQPSATQETESDPEPQATEAGEIPFGIIGTAPEDIPILPDASEMMASPNSVMYTTQ
                     LSKDEASQFYVTEMKKLGWSLTPNASINGDVANILSFTMDGKTASIIIGTATGKVKVQ
                     VVISEN"
     gene            6079..6783
                     /locus_tag="PLE39_02330"
     CDS             6079..6783
                     /locus_tag="PLE39_02330"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HNE67187.1"
                     /translation="MIRMNKDFIAGARGSLPILIGVIPFAMITGVAATSVGLTLMETI
                     GMSVLVFAGASQMVVFQLMTAGSPWIIMVLTAWVINLRFTMYSATLAPYLQKLSTWRK
                     ALLAYILSDQAFGISLSHFVTTNEEINHRWYYFGSALIVWVAWQISAILGALLGSLVP
                     ASWGFDFAFPLSFMALMFAALRDRPTVIAALAGGFIAVLAKGLPYNTGLVLATFLGIM
                     AGYWAESIQARKGTIQ"
     gene            6780..7109
                     /locus_tag="PLE39_02335"
     CDS             6780..7109
                     /locus_tag="PLE39_02335"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HNE67188.1"
                     /translation="MSAASIWLLFLAIGLGTFTLRFLFIYLFGKIEMPDWLRRALRFV
                     PAAALAALVFPALTHPTGHLDISLQNFRLLAGLGGAVVAWKTRNVLLTLIIGMILLWV
                     LEAAFGV"
     gene            complement(7211..8224)
                     /locus_tag="PLE39_02340"
     CDS             complement(7211..8224)
                     /locus_tag="PLE39_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNE67189.1"
                     /translation="MKTFVYIDHFKGDVQPASWEALGLAKSFGTATALVFGTGADDVA
                     KAALEYGADEVLLADDSLLTDYRAETYASTLSALASSHSPNLILLPTTARTREMAAMA
                     AIDLNTGVLTDVSGLEANGDSFVATRPIYEGKLIEKTVCSGKPVMATIRGRAFPKPER
                     AAGKSGTITKVSAAGEAKSTVEGYSAAESAVNLGDAGIIVSGGRGVSNNPSLTPPAGM
                     DEKQAEVWRAQQGFALITDLATLLGGAVGASRAAVDGGYITYAHQVGQTGKVVSPDLY
                     IACGVSGAIQHIVGMRNAKVIVAINKDPDAPIFKVARYGVVGDLFAVVPALTEAMKKK
                     LGK"
     gene            complement(8221..8988)
                     /locus_tag="PLE39_02345"
     CDS             complement(8221..8988)
                     /locus_tag="PLE39_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNE67190.1"
                     /translation="MKIIACIKQVPDSEAKVTASGGQVSWGDAPLVINPFDEYAVEGA
                     LQQKEATGATVTALCIGPESAKEALKHALAMGADEAILVSDAALNDLDTVGAAKVLAA
                     AIQKIGADMVVFGRQTLDNGAGLTPAQTARVLGWPMLGLAGQIKVDGGSVQVERVLEE
                     GRQSVKASMPAVLSVVQSIGEPRYPSFMGIRKASKANIPVWSLNDIGASAPAPMITRT
                     ELMTPPVRDTAVEMIAGETPAEIAEKLADKIIAEKVL"
     gene            complement(9071..9349)
                     /locus_tag="PLE39_02350"
     CDS             complement(9071..9349)
                     /locus_tag="PLE39_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67191.1"
                     /translation="MTQPPLGFVLVFLLFSLLFLSNTYKLWFKTDTYYQDLYNSLTNE
                     KTPHPFKNYFLRRLQNRKRWELEQKLFSLVGLAAVIGADVLVVTAYLG"
     gene            complement(9409..9699)
                     /locus_tag="PLE39_02355"
     CDS             complement(9409..9699)
                     /locus_tag="PLE39_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67192.1"
                     /translation="MEVNALHTKIDKTIQVNSGLIVLEVPNGFPRSESNLYLVGLRGD
                     ILWKAEKPDANTLYSKVKLNEDGETFSAYTLNGHACDLELKTGKLITFTSIQ"
     gene            9785..10006
                     /locus_tag="PLE39_02360"
     CDS             9785..10006
                     /locus_tag="PLE39_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNE67193.1"
                     /translation="MEQENKTASGLLLPETAKEKPQTGQIVAVGDDESIKLKPKDKVL
                     FAKYSGTEFKYEGVDYLLLEASDVLARLN"
     gene            10387..10470
                     /locus_tag="PLE39_02365"
     tRNA            10387..10470
                     /locus_tag="PLE39_02365"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10421..10423,aa:Leu,seq:cag)
     gene            10657..>11666
                     /locus_tag="PLE39_02370"
     CDS             10657..>11666
                     /locus_tag="PLE39_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNE67194.1"
                     /translation="MAEENLNTGNIESIDIDAQMRSAYIDYAMSVIVARALPDARDGL
                     KPVHRRILYAMHELGIRANTAYKKSARIVGEVLGKYHPHGDSAVYESMARMAQDFSMR
                     YLLVDGQGNFGSVDGDSPAAMRYTEARLQKMAEEMLADLEKDTVDFGPNFDDSLEEPL
                     VLPARLPNLLLNGSSGIAVGMATNIPPQNLRELVGAINYLIDHHDTIDDVTVNDLMKF
                     VQGPDFPTGGMIVGADGILSAYGTGRGRIVMRGIAHIEETKAGRYQINITEIPYQVNK
                     STLIERIAELVRDDKIDQISDLRDESDQRGMSIVIELKRGAQPKKVLNQLYKYTALQS
                     TFG"
     assembly_gap    11667..11766
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11767..13328
                     /locus_tag="PLE39_02375"
     CDS             <11767..13328
                     /locus_tag="PLE39_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNE67195.1"
                     /translation="LYKYTALQSTFGVQMLALVADESGRGEPRTLPLKRMLQIFIEHR
                     QTVITRRSKFDLAKAQARLHILDGYLIALNNIDAIIKVIREAEDVDTAKANLVKRFKL
                     SEVQAQAILDMQLRRLAALERWKIEEEHKQVRETIAYLEDLLAHPKKILALIKSDLNE
                     LAETYGDDRRTRIAAEAQENIHEEDLVPDESVLISLTERGYIKRVAANAFKSQSRGGR
                     GVMGHTTKDEDEVMMLIPARSLNTMLFFSDKGKVYSEKVYQIPDSDRATKGIPLVNVL
                     SLDPNEKITAAMAVGDFSSHNFCMLMTSRGKIKRVSMEEFASVRPSGLIAMSLEEGDQ
                     LGWARLTSGKDEIIIVTENGQALRFSEAKVRAMGRQAAGVQGIKLKKDDAVTSMDVVE
                     KDGALLVVTTRGFGKQTPLSDYAAKGRATSGNYTIDPKALKEIGKIAAARVVQKADDL
                     TIMTSNGVTLRLKVKDVKQSGRATKGVHLIKPQDGDNVASVARISAEDLKKAGAQVLE
                     AEKVEEQPKLV"
     gene            complement(13407..13640)
                     /locus_tag="PLE39_02380"
     CDS             complement(13407..13640)
                     /locus_tag="PLE39_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67196.1"
                     /translation="MNEFDNLREDAASSYDEAPAPKYLEVKGPAPTFTAKPRSRRILG
                     MTGQQRFILSVMFMLAVCVIGFMALFITGKIGF"
     gene            complement(13668..14453)
                     /locus_tag="PLE39_02385"
     CDS             complement(13668..14453)
                     /locus_tag="PLE39_02385"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNE67197.1"
                     /translation="MNENSLKKALSKLKLGGLRYFDSIGSTNDEALAWAATEGGAADL
                     SLVVADEQIQGRGRLNRKWFTPKGSALAFSLILRSSETLRPNLSRTVGLAALSIAESC
                     SKNGLTPSIKWPNDILINGEKLAGILVETVWSGDELDLLVIGVGINVFKSSVPPADLL
                     QFPATSLEDELGTPPNREEVLSSILEALIHWRARMGTDEFIKAWENQLAFRGQQVQTF
                     AGTDVPVVGELLGLEPDGSLRLLDKHRKSIVVRFGDVSLRPDA"
     gene            complement(14504..15679)
                     /locus_tag="PLE39_02390"
     CDS             complement(14504..15679)
                     /locus_tag="PLE39_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNE67198.1"
                     /translation="MKTLFEKINAFFTSIGGRFKRKPKEVSAVNEPPTTPRGQGAKPR
                     VDRFTIISWAVTLLIVVSLLGSTLFYKSTLANAAPVVVQPTPEEAPVAGQPQVGVPAA
                     GSGGGGVLSILRRLQLKTNIPERPRYASTTYRVSRGDAMLSIADDFKLKSESILFAND
                     QLEDNPHNLKPGMELTIPPVDGVYYEWQEGDTFESVAEKFSANADDIVNFPGNDVDLT
                     DPKIEPGTTVMVPGGTRDLRNWAADLQTMGRGAGTGTGGGNATNACGGGPVATGFGWP
                     ADDHSLSGNPYGPSHLGIDISAPEGSNVYAAGAGVVTQAQGGWNYGYGNVVQIDHGNG
                     YVTVYAHLSSYVVSVCQFVGQGAVIGYSGNTGNSFGAHLHFEIRIGGQNINPYDIVQ"
     gene            complement(15713..16993)
                     /gene="rho"
                     /locus_tag="PLE39_02395"
     CDS             complement(15713..16993)
                     /gene="rho"
                     /locus_tag="PLE39_02395"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNE67199.1"
                     /translation="MKILELENEPLSKLRGMAKTLNIPNANRLKKETLAMMIREAEAQ
                     KEGIELRGGILEIMSEGIGFLRATNYRISDQDVYVSQAQLRKYDLRAGDLVIGQVRPP
                     RESERHFGLLKVESINGLEPEPGNSRRVVFENLTPIFPDKRFDLEIGHDQLAPRLINL
                     IAPIGRGQRGLIVSPPKAGKTTILKQIANSISTKYPDVHLIIALIGERPEEVTDMDRS
                     VDAEVVASTFDEPVTAHVRTAELALERAKRLVEIGRHVVILMDSITRLARAYNLVVNP
                     SGRTLSGGMDPSALYPPKRFFGAARNVEDGGSLTIIATCLVDTGSRLDDVVYEEFKGT
                     GNMELILSRKLQERRIFPAVDIERSSTRREDLLLGPDLLQRVWLMRRMFIQMITPPPG
                     GAGMDLSVATEAIITRMERAKNNIEFLENLTKDA"
     gene            complement(17452..18891)
                     /locus_tag="PLE39_02400"
     CDS             complement(17452..18891)
                     /locus_tag="PLE39_02400"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE67200.1"
                     /translation="MAKVVVIGAGFAGHTAALYLGSRLGKKHEITVISNRDVFGYVPS
                     WVWVGVGHMKPEQTIFKLKPVYDKMNVKFVHAAAKEIHPDAGDQYVLGEGVASGQEVR
                     LDYDYLVIAPGPLLNFAGTPGLGPENGFTHSICSLPHAIKTRDAYLALCDRMKKGEKV
                     KLVMGTGHPGATCQGAAFEYIVNVHKDLVKKGLRDKADILWLSNEPAIGDFGVNGVQI
                     KRNGHIMRSEEFLTSVFNDHGIRYQVQTGVTKVEEGKIHWQNYDGEEGTTDFDFSMMI
                     PQFKGQPFKYIDKNGEDITSKITNPAGFVLVDGKYGLPYPELAQTPEAWPSQYQNPIY
                     KNVFAAGIAFAPPGPISKPFTNKNNLLIAPAPPRTGMVSGVIGRVVALNIVDLIEKGE
                     MTHREPMTEMVAACIASMGDSLWDGNAVSIVMQPVVPDRKKYQNEFGRDLFTSHLEMG
                     VSGAWMKFMLHHTFLWKFKARPGWTIIPE"
     gene            complement(19152..19577)
                     /locus_tag="PLE39_02405"
     CDS             complement(19152..19577)
                     /locus_tag="PLE39_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67201.1"
                     /translation="MRRFSLLILLIALGLSACGRNKPGPLGADASADNIPDIVGSYAL
                     NATDPTGEEYGGTLTITQGGQANEYKFQWLISGGIQEGTGTLEGNQLIFTWNSLAGTD
                     QTVSGTGQYTITVDGQLYGTRTIDGLDTPGKETAYPNPK"
     gene            complement(19640..21982)
                     /locus_tag="PLE39_02410"
     CDS             complement(19640..21982)
                     /locus_tag="PLE39_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNE67202.1"
                     /translation="MATSPTKKQLIDVLTERNPSVDWKARLNSPSRRLLNFFESISLR
                     LEAPVNWLINDPRFNPLYHTGTITIFLLVVILLTGIYLTMFYPFGFTFSYQAISKIEA
                     NLIGRIIRAMHRYASDAAVIFALLHGWRTFFQDRFRGPRWLAWVTGVGMAVVVWAIGI
                     TGYWLIWDERAQLLNQSLFSILGSFKNGQTFIVDFIVGDAAGTGWVFMMIVIVLHLGL
                     SALTAFFLWLHLKRMSRAKWLPPRYWMAISVFVLLLSAALFPLGMLPPLNATQLPAEA
                     PVDLFYLFYLSTFLRGPQALFWSVLLFIVGLVTALPWILPRAKELEPVKVDLANCDGC
                     TLCERDCPYLAIKMVPRTDGARPKFQAEIDPNLCVSCGVCIGSCPDNALTFGDIPLNP
                     LWKTTLERIAQDDKSKVKVVFTCERHALHGAGKHFEDPNTYIVPLTCIAMANPNLAAQ
                     ALEAGASDVQFIGCPPEDCANREGNVWLNDRVSGERLPKLKQNFIPLVHTAWAAPADF
                     GKAIKTQIKSEPNTFKFTPNASHLRFIIPLLGVMAVVTAFQIWLSDWPTKFFNEDSAV
                     LAIQMTHHSGYPVQDVTPLPPLEPDLEQPIRITLEVDHELVFDETYTATDNHINQGAR
                     IFEHIHLPVGRHHITVKMYDRADASFEQILFDDTVNLEPQQALTINFRDVHIPDPKAG
                     EQLYYETASGVNAGCRICHSLEKGETIIGPSFYGIANRAGERVPGLTAEEYLHQSIVD
                     PNAYVVSGYPSGQMIQNFGTILTEEEIQDLIAFLLTLDGQ"
     gene            complement(22135..23784)
                     /locus_tag="PLE39_02415"
     CDS             complement(22135..23784)
                     /locus_tag="PLE39_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="b(o/a)3-type cytochrome-c oxidase subunit 1"
                     /protein_id="HNE67203.1"
                     /translation="MKQYQLSAGEKKFIGSHLLVAILALAVGSLFGPLQAFEHAGWDF
                     YVYLKPFIKSYYQGLTVHGVLNALVWTTFFIMGFLTFNVIFGLKRPLKNMALNKVGFW
                     MMVVGVVAAAIPILANQATVLFTFYPPLKANWAFYVGLTLVVVGSWVEGWGLFMTFAA
                     WRKENPDERSPFIAFASVVTALLWQICTLGVAVEILTMLLPWSLGLIEGVDPQLARTY
                     FWFTGHPLVYFWLLPAYISWYAMLPKQAGNGKMFSDSLARLAFWIFLVLSTPIGIHHQ
                     YVDPGVSPVWKFLHSMFTFGVFFPSMMTAFTVVASLEHAGRKQGGLGMFGWIGKLNWS
                     DPSVATQLLAGILFFFGGIGGLTNASYNVNLVLHNTAFIPGHFHLTVATAVTLSFMGI
                     SYWLLPYLTGRKLWNPKWAVAQAWTWFVGMIIFSNSMHVLGLLGAPRRVPLGLAPYVP
                     AEWNSRLFQVGVGGAILFVSGAIYLTVMLMTALNKEKVNEEVIVPIAESMQEVSRTPV
                     WLDRWAPWIYGALGLIVIMYGPVLFEMIKNIALTSPGFRVW"
     gene            complement(23802..24302)
                     /locus_tag="PLE39_02420"
     CDS             complement(23802..24302)
                     /locus_tag="PLE39_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNE67204.1"
                     /translation="MSTNQIHIDSYERNWMIVSAILLVLFAAAVSFAAFGMGIQVPAP
                     EQRVDPRTVATDPNSPWSNPGLREIEPGKYDAYILARAIPSWEYLPKELTVPAGSSVS
                     FYVTSSDVQHGFKIQDTNANFMVIPGQVSKLTITFDKPGTYNFICTEYCGSGHGVMYG
                     AIIVTE"
     gene            complement(24299..24436)
                     /locus_tag="PLE39_02425"
     CDS             complement(24299..24436)
                     /locus_tag="PLE39_02425"
                     /inference="COORDINATES: protein motif:HMM:NF019724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 2A"
                     /protein_id="HNE67205.1"
                     /translation="MAKKDKEDDEFRPTGTIAVLAIFVVTLIAIWATIYMILVGRGGT
                     L"
     gene            complement(24549..25136)
                     /locus_tag="PLE39_02430"
     CDS             complement(24549..25136)
                     /locus_tag="PLE39_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNE67206.1"
                     /translation="MKTKILLGTFAIILGLVTGVYFFRPHTFHGTVIQSPETSYDFNL
                     TGGKGDVSLSDFRGKLVLIYFGYTFCPDVCPATLGQVNQAMKKLGSKAEDIQLIMISL
                     DPERDTPAKLEEYVAHFNPTFLGITGTQEQVDTVASLYGIFYEKKEGSAASGYLIDHT
                     ATLMVIDREGYLKLVFPFGVTADEIADDLKYMLKQ"
     gene            complement(25150..25824)
                     /locus_tag="PLE39_02435"
     CDS             complement(25150..25824)
                     /locus_tag="PLE39_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNE67207.1"
                     /translation="MNKYFLGSFYGLFGLIVVSAFAFKVFQPIKVLPRMQLSPAFSLT
                     DQDSNRLTSEDLRGQFVLYTFEYTNCPEVCNSINDTMLEVQSRLGEVELDGISVAFVT
                     ISMDPERDTPDALNAYAQSIGADTNRWKFATTTNEAMLKTIIGSGFETYYEKKEDGSF
                     AFDPSFVLVDGWGIVRAEYRYATEVSNADRILRHLGVLAEEVRNSKGTTKLAYEAAHL
                     FLCYAP"
     gene            complement(25821..26324)
                     /locus_tag="PLE39_02440"
     CDS             complement(25821..26324)
                     /locus_tag="PLE39_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNE67208.1"
                     /translation="MLRINRQTDYAVRVILALSKKEEGKRVSTSEVGREMLIPPALLQ
                     RIVAELASGGFILTQAGRDGGITLAHSPKQITLLQIVEHFEGELYLSDCVLKTGDCPF
                     ELRCPVHCQWVRLKNMLRNEMAQTTFQQLVDEGQQIEANLIKNASSAPKPDSAPLKIL
                     LPEEIPA"
     gene            26569..27639
                     /gene="lgt"
                     /locus_tag="PLE39_02445"
     CDS             26569..27639
                     /gene="lgt"
                     /locus_tag="PLE39_02445"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNE67209.1"
                     /translation="MDPVIFQFKLFGGEIVLRWYGVLVMLGAVVGAWLAEKEITRRGQ
                     NGEVIWDAMVWVLPAGIIGARLWYVLNSILGGSSYYTDNPGKILQVWEGGLHFFGGLL
                     FGALALYFFLKANGLDFWLFLDAIAPAVFIGQALARPANFINQELYGQPTKLPWGISI
                     DASHRLAQYADLSQFPVETTRFHPTFAYEMILNILLALLLLWVARQYEEKLKPGAIFA
                     GWLVLAGLARAFIEFFRPDQPHIGDSFVTFTMLVSLLMAVAGAVMLMVRYGRLQLAFA
                     ANWEEEYQLKKVEAAPAPKRKTSVASTAKESDEETEIEIQDEDEEMEETPAPVKKRKT
                     AAATTSTSTTKKKSTTKSKTSK"
     gene            27753..28406
                     /locus_tag="PLE39_02450"
     CDS             27753..28406
                     /locus_tag="PLE39_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67210.1"
                     /translation="MNRLFACFSLVILFTSACTVAAPSGTATQDQAVPSGTVLFQDDF
                     ANLGSGWSRFSAAEGVMDYDSGSFRILVNALDTNFWSTPQKNFTDVRLEVDAGKLGGP
                     DENRIGLICRSSGADYYFFIITSDGYYGVGLFSGGQAVLLGQNEMLTSENINKGLAVN
                     HLRADCTGDQLTFYVNGFQIAAVQDATLKSGDVGMLVGTFAKPGVDVILDNFVALKP"
     gene            28563..29474
                     /locus_tag="PLE39_02455"
     CDS             28563..29474
                     /locus_tag="PLE39_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNE67211.1"
                     /translation="MTAQIIDGTAIAQKVRDEVKEKVAARIAAGKSQPGLATVLVGDR
                     VDSAAYVGMKQKACAELGMTSYHHPLPADVSQEDLEKLIKELNADPKVNGILVQLPLP
                     SHLDEERVLQLISIEKDVDGFSPINLGRLAQKGREPLFVPCTPYGCIYLLKEAGVTIK
                     GANAVVLGRSNIVGMPAALLLVKEDATVTVVHSRTKDIPGVLRQADIVIAAIGRAEMV
                     RGDWLKPGAAVIDVGINGIPMLNPDGTPVISQKTGKPRQKLVGDVNYEEAKEVAGFIT
                     PVPGGVGPMTIAMLMNNTLRAAEIQQP"
     gene            29556..30467
                     /locus_tag="PLE39_02460"
     CDS             29556..30467
                     /locus_tag="PLE39_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNE67212.1"
                     /translation="MKKFLKWVGIVLGILAGLVAIGLVIIYVQSESRLTHVYEIPAEQ
                     VVIPTDAASIENGKHIFQFRGCEACHGENLEGTVYLDDPAVGRVISSNLTSGLGGVGS
                     AMSDSDWVRAIRHGIRPNGTAYLFMPSTEFYYLSDDDLGDVIAYIKSVAPVDNDLPAS
                     EISFTGRIVMTLVKDITFIPAELIPHDSARPTAPKAGTTPEYGAYLTQSCHVCHGLNM
                     SGGVIPGFPSTWPPAPNITFGGGSALPTWTEEEFMNTIRTGKTPQGKELRAAYMPWNS
                     YKFMSDDELKAVWAYLQSLPRLEYGNR"
     gene            30617..30973
                     /locus_tag="PLE39_02465"
     CDS             30617..30973
                     /locus_tag="PLE39_02465"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNE67213.1"
                     /translation="MTEISSHTRKFQKELNAGTSSLVLLSVLSRSKEPMYGYQIAKML
                     EESGPEIPMMKQGTLYPVLRSLEENGLLESTVEPSVSGPPRRYYKITNDGREALKEWA
                     EIWKITKKFVDANLKG"
     gene            30978..31862
                     /locus_tag="PLE39_02470"
     CDS             30978..31862
                     /locus_tag="PLE39_02470"
                     /inference="COORDINATES: protein motif:HMM:NF025168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HNE67214.1"
                     /translation="MFKTMEEYLDALKTEMKDADPALVQDAQADAREHLSTALEAARE
                     ANPAVSEADALGKIIEEYGTPEETASAYREVERRTSPALKQTTKPRSALGRFFGVYTD
                     PRAWGALLYMLIAFVTGVFYFSWAVTGLSVSISFLIFIFGFPIALLFLLSVRGLALLE
                     GRLVEALLGIRMPRRPLFSHQGMKWFDRLKALLTDKATWLMLVYMIVQFVLGTAYFIL
                     IVMVLSISLSFIALPFIQEVLHQGAVYNNGVRYYFPSWSYPLLVLGGLLLWTTFMNII
                     RGVGQLHGRLAKWLLVSE"
     gene            31867..32769
                     /locus_tag="PLE39_02475"
     CDS             31867..32769
                     /locus_tag="PLE39_02475"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNE67215.1"
                     /translation="MKQNSSASGKFMKFLQFPVTRFFLGFFLIGLVTSIAQIGTEVWP
                     GHVNNLAQIASVLIIVTIASLAYYGFVRVIEKRAVTELSPHQAVRELAGGAALGALLF
                     SATIGILWLFGFYKVSGTNAWTVLIPGLVLSISSGVIEEILIRGLLFRIMEESLGTWI
                     ALAISAVIFGFLHLANPNATLWGAIAIAIEAGIMLAAAYVFTRRLWLPIGIHFAWNFS
                     QGAIFGVAVSGNEVQGLLQSTLSGPTLLSGGAFGAEASIIAVMICLLAGIALTTLAMK
                     KGNFIQPFWAKHKPELKQENATPA"
     assembly_gap    32772..32871
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            32947..33843
                     /locus_tag="PLE39_02480"
     CDS             32947..33843
                     /locus_tag="PLE39_02480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNE67216.1"
                     /translation="MNPYLAAAQAGKNDWWRYLISFFTILTIWFVIGSIPIILLLGYV
                     MMDGNPATNFTQSGFTGISVLLDFSATMLTFFPFIAATIFSIRVIHARPLRTLITSET
                     NVRWHRILTGAAAWFGVAALISITEALLYPGRYVFSLQPLLLLVYTLLALILIPIQTS
                     AEEFFFRGYLLQWIGLKLKNKWLLAFINGVLFFIPHMVNPEMAVNGILMGLNYFAFGF
                     FACLITLQDNGMELALGLHAANNLFGALFANYTVTALPSPALFTVQTLDPVYGLISTT
                     IGMIVFYWIVFRKQPQPAVILE"
     gene            33992..34549
                     /locus_tag="PLE39_02485"
     CDS             33992..34549
                     /locus_tag="PLE39_02485"
                     /inference="COORDINATES: protein motif:HMM:NF019826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HNE67217.1"
                     /translation="MTSTIDREKKILEHLQKQGSASIQELVEVFNVSNMTIHRELNKL
                     AEAGLIQKKHGGVALVTQSPTAGINQCIMCNKTISERTVFIVRTTSGEQKRACCAHCG
                     LMVQSQTQDAAQSLTADYLHGHIISTTQAVYVLGSDLKVCCVPSVLSFGSQEDAEKFQ
                     KGFGGTLATMKDAIQFIHGMMHAHQ"
     gene            34595..35086
                     /locus_tag="PLE39_02490"
     CDS             34595..35086
                     /locus_tag="PLE39_02490"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HNE67218.1"
                     /translation="MKRILMMLVLVGTLLLSACGSTMGDEHADGTDIEAHDFWVRSAL
                     KDGNSAAYMLLHNHTEADDELVGASSDAAASVEIHLSQMKSDGTMEMIPQEAIPLPAE
                     GELELKPGSYHIMLIGLTKDLNVGDEITLTLHFKNHEDITLTIPVMEAQDMGGSGMDG
                     HMP"
     gene            35388..35876
                     /gene="nrfH"
                     /locus_tag="PLE39_02495"
     CDS             35388..35876
                     /gene="nrfH"
                     /locus_tag="PLE39_02495"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HNE67219.1"
                     /translation="MSKTPFFIAIAATVLAFGYFAFVTDAASYGGSAPETCANCHVMD
                     AQYENWYHGAHENFAKCTDCHLPHDNVVTYYAEKGRQGAKDVFAFVTGNIPVQIRASE
                     KTKGIIQANCVHCHEDTVEVVVMNAQPFDRYCWDCHRSVAHGDRGASAIPFQDSTLYP
                     IK"
     gene            35888..37225
                     /locus_tag="PLE39_02500"
     CDS             35888..37225
                     /locus_tag="PLE39_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HNE67220.1"
                     /translation="MKNYTNLILAGLVIVLAAALVGVLTFVKNQPAQERATEPLVEIA
                     AMEPDSAKWGVNFPNQYDSFMKTQTNNIDTTYAGSSQFSWLERDPRQVILFAGYPFSK
                     DYNDDRGHANALEDVRATGRLNLDDSNPKHTPATCYSCKSTDNPGLWAEMGMEAYDKM
                     SFNEMTPNINNAIGCANCHEAETMRLIVTNPALEEAFERQGKDWTTFTRQEMRTVVCA
                     NCHVEYYFKGDGKYLTFPWDNGTKVDEMLAYYEEAGFKDWEYPDTGTPMLKAQHPEYE
                     FFTADSTHYKAGVSCADCHMPYTRDGAAKYSSHDVHSPLLNAEEACGQCHTDTEFVVN
                     RVNTIQEQVATTKITTEDAILDAVNAIKAAVAAGNADPALLDQARKLHREAQYMWDIV
                     SAENSTGFHNPEYALKVLAEATNRARQAQMLAAQSINDTSLLATGTYYATPAP"
     gene            37327..37773
                     /locus_tag="PLE39_02505"
     CDS             37327..37773
                     /locus_tag="PLE39_02505"
                     /inference="COORDINATES: protein motif:HMM:NF040341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HNE67221.1"
                     /translation="MKKLILLLLALILTACSAGTLNEFDKNLAKWESAKITHYRYDVA
                     IGCFCAFRDDMPMTIEINNGEIVSITSVKGNIITPSDGLYDVVKSYAGMEALFEQLKG
                     AFEDADRVEVIYDTVNGYPTSIAIDQIEAAVDDELYISVENFEVLK"
     gene            complement(37837..38514)
                     /locus_tag="PLE39_02510"
     CDS             complement(37837..38514)
                     /locus_tag="PLE39_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE67222.1"
                     /translation="MKTIEVSHENRIKTLKGNEYFDNLPEPMLKDISEHMHLREYQRG
                     DVLFWEGDPCDGLYIVEQGSAKIFRLSPQGRQYIVRILQEGDTFAEVPAFDGAANPVN
                     VEALEACRVWVIDGELLRRLVELHPHFAQKVLANFGRMLRGMVRMVSEMAFYQVTHRL
                     ARLIAEMPQERSAPHWTQEQLAARLGTVREVVARSMRELERSGAIKVEDRRIQIVDQN
                     IFSQWME"
     gene            complement(38522..39031)
                     /locus_tag="PLE39_02515"
     CDS             complement(38522..39031)
                     /locus_tag="PLE39_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNE67223.1"
                     /translation="MQPEPRREMVTWQEIDRLIDHLLPQFKREFTAMVMITRGGIIPG
                     GLLADAMDITHILTAAVDFPAQSKTEKSNLMVWPEFIQFPAEDKLRGRPTLIVDDVWG
                     SGRTITAVKNRVSAAGGFAETCVLHFNPYRNLFGNARPDYYAAVTDAYIVYPWEVDRG
                     ADKVLLGEL"
     gene            complement(39103..39999)
                     /locus_tag="PLE39_02520"
     CDS             complement(39103..39999)
                     /locus_tag="PLE39_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67224.1"
                     /translation="MQSFSRGWAFLKQAWEMAFKDKDLLKPSIYALIVGMIVSVIGII
                     PIVGAAILFGDTQIGNIILFVLGGILVFVQFVVTYVFSGMTVHLIYGYLTEGDGDMGK
                     AWAIVRRDFFDILTLAAASTAVNLLRSLANRNRRGGVAASVARAATGLLETLWTEAAL
                     LILPAMIIDDLNLKDGMQRILKITKENLLLVGISTVGVRWVTGLIGFVFGAIGLVIAF
                     IIGGGAAFVSGGSTAISIIGIVIGALIFFTFVMLANLFSSYTNTAYHTCLYIWARQVE
                     TARAHGSAEQVRAPAPLAAALA"
     gene            40109..40480
                     /locus_tag="PLE39_02525"
     CDS             40109..40480
                     /locus_tag="PLE39_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNE67225.1"
                     /translation="MKKTILLLIALALSACSAMAKAEPTPTPDPLVLQGQQVFNAKCA
                     TCHALVPDTIIIGPSLNGIATRAETRVEGVNAEEYIQLSILRPGDYVVEGFNNVMITN
                     FAKELTNEDMNALVAFLLTLK"
     gene            40502..42748
                     /locus_tag="PLE39_02530"
     CDS             40502..42748
                     /locus_tag="PLE39_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HNE67226.1"
                     /translation="MKVWMKRIFSGLGILLLIGILYAAFAPLPQDEVLPPEKWGAGSS
                     SVEPAYSGLQRDFPATNETDANATTPEKAELGRLLFFDPILSKNDDMSCATCHNPDLG
                     FGDGMQQALGANGKTLPRNAMSLWNVAYNTNFFWDGRAATLEEQMHTPITNADEMAGS
                     PEEIESQLKAIPAYVSLFEQAFGSKDAVTYENVQNAIAAFERTLITNNSPFDRYAAGD
                     VNALTPAQRRGLATFRSAGTRCFECHAAPTFSDDSFSVTGVPDLPGQEHDAGRAEVES
                     ESLDGAFKAPSLRNIALSAPYMHNGAFATLEEVVEFYAKGGGRDDGVENVDIHVLGFD
                     LTKQEKSDLVAFMYALTDETNLPEFPESVPSGLPVVAHVENPAREIVSQMNAGGTESV
                     STSTHEAVTLRVEKGQSIQSVVDQALPGDTIEVPYGIYKEHVIIDISDIKFFGIPNEE
                     GQWPVIEGEGKGADGVIASGNNFEMAFFAVKNFTSNGVLVEGAKNVYLHDMYIENTGV
                     YGVYPVRCTDVLIERIEGTLMNDAAIYAGKSENVIIRDTLTYGNVIGVELENTVNGEV
                     YNNVAHDNTIGIFIDLLPQLPSKVSMYTKVHDNVVENNNGANFGKPGTAVSLIPPGTG
                     MLILAADHVEVYNNTIRGNKTGGMAVFNLTIGFSEEEIDVGPNPEHNYIHDNIYENNG
                     YDADKFVKDMLGQGFDIIWDGTGADNRFDEPNASSSFPPVLPSKNLPAPLYNLYWRAL
                     NFVVGLVG"
     gene            42939..43520
                     /locus_tag="PLE39_02535"
     CDS             42939..43520
                     /locus_tag="PLE39_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67227.1"
                     /translation="MKNALLVLFLVSVLTACTPAITSAPTETILPMIVLSSTPIPTST
                     LIPATETPSPVPTDPIILMITPDSIQVERWREYQTALAKTIMSYELALCEWEVLGRSE
                     NEIYVWATCRILDNGSDSPAVIHLGVDGAIQKVEIPGTNWSSDIREMFPIEIQEIFLN
                     NLMNYQQLSDHLTWRIEHPEESPLIVLSSTPAP"
     gene            complement(43554..45989)
                     /gene="priA"
                     /locus_tag="PLE39_02540"
     CDS             complement(43554..45989)
                     /gene="priA"
                     /locus_tag="PLE39_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNE67228.1"
                     /translation="MFARVAVNVSVISGLFDYAIPNELALQIQAGSFVIVPFGKQTVQ
                     GIVTALIDTPAVENPKPIMELLDPAPVLTQPQLALAIQLAESTLNPLAAIISLMLPVG
                     LSQQADVHYELRIANYEDKTSTVATRLLTLLRERGPLRGRQIDSHFSRVDWRKTAQAL
                     VRKGILATKHVLPPPRVRSKFVRVAQLSVTPEAAEAEMDSLGTKQTLARRQSALRYLI
                     QQPEAINLSWVYAETGCNLADLQELEERGLIRLFENEIFRDSLQRIENGKEEHGRQFE
                     LTSEQSSSLEKITHSSFILQPSPFLLHGVTGSGKTEIYLRAAEETIKRGKQAIILVPE
                     IALTPQAVRRFLARFPGQVGLIHSKLSEGERYDTWRRARSGKLKVIIGARSALFAPLP
                     NIGLIVVDECHDSSYHQTEPPFYNAAVAAQEYARLCGAVCVLGSATPTVEQRFAAENG
                     TLTKLTLPNRVTDSALPPVQVVDMREELKTGNRGIFSRALAESLAETISRGEQAILFL
                     NRRGTATYIFCRDCGFVLKCPNCDTPLTLHVGTFERSNVPTLLCHHCGYTRQKPKTCP
                     NCNGKQIREYGLGSEKVEEEVQAMFPGVRTLRWDWDTTRHKDAHEMILTHFSNHQADV
                     LVGTQMLSKGLDLPLVTLVGIVLADVGLNLPDPFASEKVFQTLTQVAGRAGRSSLGGK
                     VVLQTFMPEHYAIQFAAGHDVNGFYERELDYRKQLGYPPFAKLVRLEFRDADPLKAES
                     DSQKAADKLKLRIETENRRQTELIGPVPAFFSKVDGIHRWQIILRGPDPVSLLRDLKL
                     SDCRVEVDPISLL"
     gene            46031..46465
                     /locus_tag="PLE39_02545"
     CDS             46031..46465
                     /locus_tag="PLE39_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67229.1"
                     /translation="MLTIIMILGGIAILASVILLAFMFIKSNEVNLTGKTEDKPEWMK
                     SNPPKETVKATRAEGEGVTLFDHDAGEKIASPFAEQIEDILRAKLESDPFNKFDIDFG
                     SAPDGSLEIWVNGSMYQSVDGLPDEGLKNAFRNAVKEWERLR"
     gene            complement(46495..47337)
                     /gene="amrB"
                     /locus_tag="PLE39_02550"
     CDS             complement(46495..47337)
                     /gene="amrB"
                     /locus_tag="PLE39_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNE67230.1"
                     /translation="MTTLDLRPSPIAGQWYDANPTALARSVDDFLAHASVPQLNGEVL
                     ALIAPHAGHLYSGAVAAHAFATLHGLAPELVAVLSPYHNYAHFPLITTKHQAYFTPLG
                     NLSVDQAALAELQTHLEIPITAIANDREHSLEIELPFLQRVLKNEFKLLPIMIRAQDV
                     TIAQKLGHALAQTLKGKNAIIVASTDLSHFYNQKTAGILDAEMLRRFESFDPESVFDA
                     EQTGVGFACGHAAVASMLYAARELGADKVQILKYATSGDVTGDTSSVVGYGAGVVLRP
                     STSH"
BASE COUNT        12009 a        12016 c        11840 g        11359 t
ORIGIN      
        1 catgttactg acctcggctg gaaggatatc tgccacggat gcgccgatga tctcttcggg
       61 ggggatgtaa aaacggctgt cgtctcctgc cttgaaatca ataaagcgac ctgcctcatc
      121 catgcggaaa atcaaatcgg gaatggcgtt caaaaatgcg cggttgcggg cttctgattg
      181 acgcagtttt tcttcggcaa gcaattgctc ggtcagatcc accacagcac caatgctgcc
      241 tttaatgtga ccgtccttga tgcgcggaga acggcttacc agtgtattga ccacggttcc
      301 atctttggca atcaggcgca tctgggcagt atatgagttt tgggtttcat cctgcggtat
      361 atcttcgatg tgcatgaatt cctgaatttt cctgcctgtg atctcttggg ctgtgtattt
      421 cagcatcttt gcataggcgg gattggtata ttccaaacta ccatccaggt cggcaacgat
      481 caacccgtgt gcaacggttt ccatcacttg aagggcaaag tctctttcct ccacaacttc
      541 acgctggctg cgttggcgaa tcaacgtatt ggcaacgacc ccggataaca tgcttagcag
      601 attaacttct tccttggtcc agttgcgctc atgggccacc gagtcaaagc cgataaaccc
      661 gaacaaaccg tggtcagatg tcatcggcgt taccaggacg gattgaatcc cctgctcaga
      721 caggatttga cgttcagacg ccgcttcttt cggcatttct gatacacgcg gaataatgat
      781 gatctcgcca ttttcaagca attgcatcca ccagggcaca atttcccttg gcaccccttg
      841 caggttgtcg atctgcggaa tgatctcgtt tgcgcaccac tcaaaggtat tgttcatggc
      901 caggccatca tcggtaaatt caaaaatgta ggtgcgatcc actttaaaca attcgcccag
      961 cctgcgaagc gtatcgtgga cagccgtatc catttcgctg aacggcacat tcatgaactt
     1021 ggccgacaaa tgtgaggtta attcttcaaa agtcagcttt tggtgcaggc tggcggtcat
     1081 gcggttaaac tggctggcaa gttcattgat ctcatcagtg cttccaaaca atttccgaat
     1141 tgaaattctc tgggtcaggt caccattctc caccttgatc aacccgctgg aaaggacgct
     1201 taagcggtca aggattccgt tttgggtcac acggtagtac caataaaacg tcaggaggaa
     1261 gcctccaaga ctgaccgcaa ggatcaggga catgtcttcc tgtgcgcggg cataggcggg
     1321 cgtgaaatcc aactcgatcc ataaaaaacc cgtcacatca ttgagattgg ctgtgtaggt
     1381 ctctccgcgc aagggtctca ccacgatcaa tgaccttccc tcaatttgtt gttccttgcg
     1441 ttgattcttc agggaatcct ccaacagagg agagttgaac acctgcccca cctgactgtg
     1501 atcattgtca gccagaataa cgcccttctc atcgagcacc ttgatggaac tgacattatt
     1561 cagggtggat gtcttctcga tcagcctttg gtaattaaac gtagcatcgc cgccctgggc
     1621 cgcaatgacc accatgctat aggcgatctg gtctgaaagg ttttcagcct cggcaatggc
     1681 attactcaaa atccgctggc gggaaaccag ccaggtgatc gagacgatca gcagaatccc
     1741 agcaaagcca accaacccct gataaatggt gagttttgtc tttaaagaat tcatatcagt
     1801 ttattctgat ctcgctcatt ggccgtcctt gaacaaggtt agaacaaagg tcggctccag
     1861 catttgttga acatcaggca tagtagtgat cacattgttg tcggccagga acacgctgat
     1921 ttcctgagca gcggcatata cacgaccatc ggaattcacg ccaaataatt ctgcatttga
     1981 actcaaatca agatacctga tacccataac atgtgtggaa aattcctcag cgctgatgcc
     2041 ttcggccttg cccatgatcg tgtaggcttc ctgcggattc tgcttaatat attccaccgc
     2101 atcaaaataa gcagccacga tcttttgcac atcgtcaggt ctggcgtcaa tcagtttggc
     2161 attgaaagtc atggtcgtaa caatcgtctc agggtattca cggctggtga tcaacacttt
     2221 tccaccgatc atttcagccg tgctggtcag gtacggctcc catgtcaccc ctgcatcgat
     2281 ctgaccgtcg atcatcgcct gttgaatttc ccaggcgggg atactgataa aggtcacatc
     2341 actgaccgca actcccgctt tttcaagaat cttcaaagcg gataaatggc cgacggttcc
     2401 tatctcaacc ccgatcttgg taccgcgtaa atcagacaga ttctcaatcc cagcccttgc
     2461 caccagccca tccccaccga aggaataatc ggtcaacaga acgacgcgca catccctgcc
     2521 tgcggccata tttcgcagtg agtcatccag ggtatgcatg ccgccatcaa cattctcgtc
     2581 acgcagggcc tccagtgaat cactgtacgc tgagaaacgt acaagttcca catccacgcc
     2641 atatttttca tagtatccca attgagcggc aagttcggct ggttccaccc ctgcccatgc
     2701 agaaatggca atacggatcg gtttcttttg tgaagcacag ccgcttaaac caatggaagc
     2761 caggaggagc aagatgaccg cccagcgaaa agtcttattt gtatgcattt gacatccctt
     2821 cttttacgga atttcagtgg atccaggcgg aaccatcatt tgaacacgac accccctggt
     2881 actgggaaaa atggtgacat ctccaccaag ggaccttgca aaaatacgag caaccgtcag
     2941 ccccacccca aggccattat aggcacggca gtcaccctga tctatctgga agaaacgatt
     3001 gaacaccttt tctgaatatt gctgagcaaa tccatggcca taatcggtca cggttaacac
     3061 gaagcctccc acttcattct cctccagatc gatcaagatc gttgtcattg gattggagaa
     3121 tttgaaggca ttgtccacaa gatgcccaat tgcgcgcttg aactcacggc gaggcgcatg
     3181 gatggtcacc gttggatcga tcttgacctg ggttttgata ttcttttccg catacaaatc
     3241 ctgcaggaat ttgatgggct ccatgaaatc tgccttgagg tcaacttcct gccgcaaggt
     3301 attgaccatg ccacggtcat aattattcag gaagatcaga tcgtcgatca aaccgttcaa
     3361 tttatgcgac tgtgagaggg cggtttccac gaacagtttc aagtcatcaa tgttttcgta
     3421 tttgcctcgc agggccatat ccagcgcaag caggatctgc gtcatggggg tgcgcagttc
     3481 atggctgatg ttatgtgaaa tctcccgctc gaagcgcagg atctcctcat ccattttcct
     3541 gcgcatgtca ccctgccctt tttcgtagcg attgaacact gctgaaatac gggccaataa
     3601 ttcgcgatgg tcaaaaggct tggtgatgta atcgtccgcg ccgttgttca aacctcgcaa
     3661 cttatcagtt tgagatgccc tcgcgctaag aaaaagaaaa gggatgtctt tcgtaacagg
     3721 gtcttcgctc aatgtttccc tgaccttgaa cccatccatg aaaggcatca tcacatcaca
     3781 gatgatcaaa ttgggctgca ccttccttgc catttgtaca ccagccattc catcgcttgt
     3841 gctcactgtg ttatagcctt catgctcaag cagagccttc acaccaacca gcaagtggaa
     3901 ttcatcgtca atgacaagta tggttttacg agaatcggac atgtactagc actcctccat
     3961 ttacgaatac gaaataactt ttttcaaaaa gacatccacc acatgcggat caaaactttt
     4021 cccagcctca ccaaggatgt agtctcctgc tttttcacag gaccaggcct ttcggtaggg
     4081 acgatcgttg gtcaatgcat cccatacatc caccacggaa aagatgcgtg cagagagtgg
     4141 aatgttatcg ccagccagat ggtcagggta cccttttcca tcccagcgct catgatgata
     4201 caggggaata tccagggtgg ttgatggcag ccccagaggc tcaaggatct gactggcgat
     4261 ttgcggatgc atcatcatta cattgcgctc ttccttggtc aattcaccgg gctttagcag
     4321 aatgttatct ggaataccca ccttgccaat atcatgcaag atcgcccccc agcgaatatg
     4381 gatgagttcc accccatgaa ttccaagttc tatggcggtc ttttccgtca aattcatcac
     4441 ccgccaggca tggtcttctg tttcgttatt ccttaatccc agcatatgaa ccaggctgtc
     4501 tacaaacttt tcccaattca cttcagaatt ccgtagaatt tcggtgatct catttctcag
     4561 gtgttgaatt tcatccttgt ccgtggcaga agacagtaca ggtgctttct gtttgcgcct
     4621 caaaatagtt tttacgcgtg ccagcaattc atctttcaca aatggcttga tgacataatc
     4681 atccgcaccg gaaagcagac catgcacctt gtctgtggaa gaagagcgag ccgtcaaaaa
     4741 aatgaaagga atattggcag ttcttgggtc ctgaccaagg atcgtgagca cctccatacc
     4801 gctgggaggc ggcatcatca aatcacaaat gatcaagtcg ggagtggatt cgcgggcgat
     4861 atcaatgcct gtgcggccgt gtctggcagt aaacacacga tacccctcat atcgcaacac
     4921 tgcctccaac cccaccagca gggcggggtc atcatcgatc agaagtaaag aagttttatt
     4981 ttccatgagt ttggttgggt ggatggttta ataaacaacg agcccggaat catccaggct
     5041 cgcatatttc aaatgaattt ggcgcggaat ttcatgatga tacctggcag ttttgacctt
     5101 taatccctgt gaattttgtc ctgtcagcat tcatcaggtt ggaaaacgat tttcggatta
     5161 tagcatcgag gttttcgggc aacatttaca gactcgaaaa ccagcttata acatctgcaa
     5221 agactcctgc aaggattcag agatgctttg gccgattcgt caaaatatct tgcatcattg
     5281 ttgggattcc cccgctggta aagaaaagat gtattgttat tcgatcatca ttggaaagtt
     5341 cttatttttt caacatcccc cccatcaaaa atgaaaaccc gtaaaagttg tttatacttt
     5401 tacgggtcac atcagttaat tttcagaaat cacaacctgc actttcacct tcccagtcgc
     5461 cgttccaatg ataatggaag ctgtttttcc atccatcgta aaactcagaa tattggccac
     5521 atcgccatta atggatgcat tcggggtaag agaccagcca agttttttca tctcagtaac
     5581 atagaactgt gaagcttcat ctttcgataa ctgggtggtg tacatcacag agttggggga
     5641 agccatcatc tcagaggcat ccggcaggat ggggatatct tccggggcag tgccgatgat
     5701 gccaaacgga atttcacccg cttctgtggc ttgcggctca gggtcactct ctgtttcctg
     5761 tgttgcagac ggttgcgatt cctgcccgcc tgtgaatact gtcggcacca ctggaggcgg
     5821 cgtgctcatg gcctccgtca ctgcattaca agccagcgaa agcatggaaa aacaaagcaa
     5881 aacaaataac attcggtaca ttgatccgta tcgcattcca aacctccaaa atgagtttca
     5941 ttcattgtag cggacgaaaa gaaaaagtca acaaaaatgg gaaggatata ccaatcgccc
     6001 ctgctccatt gaatttgcct gccgtatttg agggtagaat acccatccaa aactttttga
     6061 agggaatgtt ggtaaacaat gattcgcatg aacaaggatt tcatcgcggg ggcacggggg
     6121 tcattgccga tcctgatcgg ggtgatcccc tttgccatga tcaccggcgt ggcagcaacc
     6181 agtgtggggt tgaccttgat ggaaacaatc ggaatgtctg tgctggtttt tgcaggagca
     6241 tcacaaatgg tggtcttcca actgatgaca gcgggcagcc cgtggatcat catggtattg
     6301 accgcctggg tcatcaatct gcggtttacg atgtacagcg caaccctggc cccctatttg
     6361 caaaaactat ccacctggcg aaaggctttg ctcgcataca tcctatccga ccaggctttt
     6421 ggcatcagcc tgagtcattt tgtgactacg aatgaagaga tcaatcaccg ctggtattat
     6481 tttggttcgg ccctgatcgt ttgggtggca tggcagatca gcgcgatcct tggcgcattg
     6541 ctcggttcac tcgtccctgc cagttggggt tttgactttg ccttcccgct cagttttatg
     6601 gctctcatgt ttgccgcatt acgtgaccgc cccaccgtaa tcgcagccct cgcaggcggc
     6661 ttcattgccg tgcttgccaa aggcctgccc tacaacaccg gtctggtact ggccaccttc
     6721 ctcggcatca tggcgggata ttgggcagaa agcatacaag ccagaaaagg aaccattcaa
     6781 tgagcgccgc ttccatctgg cttttattcc tcgccatcgg cctggggaca ttcaccctgc
     6841 gctttctatt catctatctg tttggaaaaa ttgaaatgcc cgactggctg cgccgtgcgc
     6901 ttcgattcgt ccccgcggct gctctggcgg cactggtatt ccctgcgctg actcatccca
     6961 cgggccatct cgatatttcc ctgcaaaact ttcgcctgct ggcgggcctt ggcggtgccg
     7021 tcgttgcctg gaaaacccgc aacgttctcc tcacgctcat catcggcatg atcctgctat
     7081 gggttctgga agcagccttc ggggtatgag cgctcaaaat gactgccact gaaacgtttc
     7141 cgcgaatagt aataaaaatt ctccgaagcc aagatggctt cggagaattt ttattactcg
     7201 tatcttgctc ttactttccc aatttcttct tcatcgcttc tgtcaacgcg gggacgactg
     7261 cgaacagatc ccccaccacg ccgtagcggg cgactttgaa gatgggtgca tcggggtctt
     7321 tgttgatcgc cacgatgact ttggcattgc gcatgccaac gatgtgctgg atcgcgccag
     7381 aaacaccgca ggcgatatac aagtcagggg aaaccacttt gcctgtttgg cctacttgat
     7441 gggcataggt gatgtagcca ccgtctacgg cggcacggct tgcgccaacg gcaccgccca
     7501 gcaatgtagc gaggtcggtg ataagcgcga aaccctgctg ggcgcgccag acttcggctt
     7561 gtttctcgtc catgcctgca ggcggggtga gcgatgggtt attggaaacg ccacgaccgc
     7621 cagagacaat gatgccagca tcgccgaggt tcacggcgct ttcggcggcg gagtagcctt
     7681 ccacggtgga tttcgcttcg ccagccgcag agactttcgt gatcgtgccg ctcttgcccg
     7741 ctgcacgctc gggcttcggg aaggcacgtc cgcggatggt tgccatgacg ggtttgccag
     7801 agcacacggt cttttcgatc agcttgcctt cgtaaatggg gcgagtcgca acgaatgagt
     7861 cgccgttcgc ttccaaccct gaaacatcgg tgagcacgcc agtgttcaag tcaatggcgg
     7921 ccatcgcggc catttcacgg gttcgagcgg tcgtcggaag caggatcagg ttcgggctat
     7981 gagaagaagc gagcgcggaa agggtcgagg cataggtttc agcgcggtaa tccgtcagca
     8041 aagaatcatc ggcgagcaga acttcatccg cgccatattc caacgcggct ttcgcaacgt
     8101 cgtctgcgcc tgtgccaaac accagcgcag tggctgtgcc gaaacttttt gcaaggccga
     8161 gcgcttccca ggatgcgggc tgcacgtcgc ctttgaagtg gtcaatataa acaaaagttt
     8221 tcatagcacc ttctccgcga tgattttgtc ggcgagtttt tcggcgatct cggcgggggt
     8281 ttcgcctgcg atcatttcaa cagcggtatc gcgcacgggc ggggtcatca actcagtgcg
     8341 ggtgatcatg ggtgcggggg cgctggcgcc aatgtcattc aaagaccaca cggggatgtt
     8401 tgccttcgat gccttgcgaa tgcccatgaa cgaaggatag cgcggttcgc caatgctttg
     8461 cacgacgcta aggacagcgg gcatgctggc tttgacagat tgcctgcctt cttcgaggac
     8521 tcgttcaacc tgcacgcttc caccgtcgac tttgatctgc cctgccaacc caagcatggg
     8581 ccacccgaga actctggctg tttgtgcagg ggtcaaccct gcgccgttat caagggtttg
     8641 gcggccaaag acaaccatat ctgcgccaat cttctgaatc gccgccgcaa ggactttggc
     8701 tgcgccaacg gtatccagat cgttgagcgc cgcatctgaa acaaggatgg cttcatcggc
     8761 acccatggcg agggcatgtt tgagtgcttc ctttgcagat tcgggcccaa tgcacaaggc
     8821 cgtgaccgtt gcaccagtgg cttccttttg ctgaagcgcg ccttcaaccg catactcatc
     8881 gaacgggttg atgaccagcg gcgcgtcccc ccatgagacc tgcccgcccg aagccgttac
     8941 tttcgcttcc gagtcgggta cttgcttgat acatgcgatg attttcaagt tgtactcctt
     9001 gtaaaaattc ttttttaccg cgaagtgtca cgaatgtttt tcttttacac acaacacttt
     9061 gtggtttatt tcatcccaaa tacgccgtca ccaccagcac gtcggcgcca ataacagcgg
     9121 ccaaaccaac caggctgaac aacttctgct ccaattccca acgcttgcgg ttttgcagcc
     9181 gtcttaagaa ataattcttg aacgggtgcg gggtcttttc gttggtcagg ctattataaa
     9241 ggtcttgata gtacgtgtcg gttttgaacc acagtttgta ggtattcgaa aggaacaaca
     9301 ggctgaatag caaaaataca agcacaaatc caaggggagg ttgggtcatt aggtctctta
     9361 cgaacgtggt tttttgtcac cgtttcagtg atacgaacaa atatctggct attgaatgct
     9421 ggtaaaggtg atcagtttcc ctgtcttcaa ttccagatcg caggcatggc cgttgagggt
     9481 ataagccgaa aaggtctcac catcttcatt aagtttgacc ttcgaataca gggtgtttgc
     9541 atcgggcttt tcagccttcc acaaaatatc ccctcgtaaa ccaaccaaat acaggttcga
     9601 ttcactgcgc ggaaacccgt ttggaacttc caacacgatc aggccagaat tgacctgaat
     9661 cgttttgtca atttttgtat gaagggcatt tacttccatg gtattcctat ccttatcggc
     9721 acttttcctg cagacttgtg tattatatcc caaactcccg caacctaccg agcggtaggc
     9781 aggattggag caggaaaata aaaccgcatc aggactgctt ttgcctgaaa ccgccaaaga
     9841 gaaacctcaa acgggacaga ttgtcgcagt gggagacgat gaaagcatca aactcaaacc
     9901 gaaagacaag gtgctttttg ccaaatacag cggcaccgaa ttcaaatatg aaggtgtgga
     9961 ttatctgctg ctcgaagcca gcgacgtact tgcccgcctg aattaaccaa caaaaaagcc
    10021 cgacgcgtct tgaaacatgc gtcgggctaa gggatgggat ggtcttccat cattgctctt
    10081 gaactcccat attgctgagt tcttctgaat tgtactgggc tacaaagagc tcagaaccag
    10141 ccagagcgag caacagaagg gcaaccactg ctgcgactac gaacattttg caactccttt
    10201 ctctgtttaa ctatataacg tttaaaaccg caaaaagaga cctcccccaa cagtactgta
    10261 atgtatatgt aacctcagaa gcgatttgca aagtaaacgc aaaggcaagt ggttttgttc
    10321 ccgcataact gcccgcttct tattgacatt ccaactattt gagataaaat acaacccgca
    10381 tcgcaagccg aagtggcgga attggcagac gcgctacgtt cagggcgtag tgagcttacg
    10441 ctcatgaggg ttcaagtccc tccttcggca caaaaaaaga cccacagttg agggtctttt
    10501 tttgttgggt ttgtgacagg taaaacaggt atgggggtgg tataattacc ctatcataaa
    10561 gacctttctt catctaatgc agacacgaag ttctccccca tcttcgtgtc tttttatgcc
    10621 aatttgacgg aattcccccc attttaagga tattttatgg cagaagagaa cttgaataca
    10681 ggcaacatcg aatccatcga cattgacgcc caaatgcgct cggcatacat tgactatgcc
    10741 atgagcgtta tcgtggcccg cgcccttcca gatgcccgcg acggcttgaa accagtccac
    10801 aggcgtattt tatacgccat gcatgagctg ggcatccgcg caaatacggc ttataaaaag
    10861 tcagcccgta ttgtcggtga agtgctgggt aaataccacc cgcatggtga ctctgctgtg
    10921 tatgaatcca tggcgcgcat ggctcaggat ttctccatgc gttatttact ggtggatggt
    10981 cagggcaact tcggctctgt agacggcgac tcccctgctg ctatgcgcta caccgaggcc
    11041 cgcctgcaaa aaatggcaga ggaaatgctc gctgacctcg aaaaagacac ggtggatttc
    11101 ggccccaact ttgacgactc gctcgaagag ccgcttgtcc tccctgcccg actgcccaac
    11161 cttttattga acggctcatc agggattgcc gtgggcatgg ccaccaacat cccgccgcaa
    11221 aatttacggg aattggtcgg cgcgatcaat tatttgatcg accatcacga caccattgat
    11281 gatgtaacgg tcaacgattt gatgaaattc gtacaaggcc ccgacttccc caccggcggc
    11341 atgatcgtcg gggcggacgg gatcctttcg gcgtatggaa caggccgggg tcgaatcgtg
    11401 atgcgcggca ttgcccacat tgaagagaca aaagctggcc gttaccagat caacattacc
    11461 gagattccgt atcaggtcaa caaatccacc ctcattgaac gcatcgccga gttggtgcgc
    11521 gatgacaaaa ttgaccagat ctcagacctg cgcgatgaat ctgaccagcg cggtatgagc
    11581 attgtcatcg agttgaagcg cggcgcgcag cccaagaagg tacttaatca actttacaaa
    11641 tatacggcac tgcaatccac atttggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnaact ttacaaatat acggcactgc aatccacatt tggtgtgcag atgctggcgc
    11821 tggtcgccga tgaaagcggg cgaggtgaac cacgcaccct gccgctcaag cgcatgctgc
    11881 agatctttat tgagcaccgc caaaccgtca ttacacgccg ctcaaaattt gacctggcca
    11941 aggcgcaggc acgtctgcat attttggatg gctacctgat cgcgctcaac aatattgacg
    12001 ccattatcaa agtcatccgt gaagcagagg atgtggacac cgccaaagca aatctggtca
    12061 agcgcttcaa actcagcgaa gtgcaggcac aagccatctt agatatgcaa ttacgcagac
    12121 tggcagctct cgaacgctgg aaaatcgaag aagagcacaa gcaggtccgc gaaaccattg
    12181 cctaccttga agacctgctc gcccacccca agaagatcct tgcactcatc aaatctgatt
    12241 tgaacgagtt ggcagagacc tatggcgacg accgccgtac gcgcatcgca gcggaagcgc
    12301 aagagaatat tcacgaagaa gatctggttc ccgatgagtc tgtactcatc agcctgaccg
    12361 aacgcggata cattaaacgc gtggcagcca atgccttcaa atcacaaagc cgcggcggac
    12421 gcggtgtgat ggggcatacc accaaggatg aagacgaagt gatgatgctc atccctgcgc
    12481 gcagcctcaa caccatgctg ttcttctcag ataaggggaa ggtctactct gaaaaggtgt
    12541 atcaaatccc cgattcagac cgcgccacga agggcattcc actggtcaac gtgttgtcgc
    12601 ttgatcccaa tgaaaaaatc accgcagcca tggccgtggg agatttttct tcgcacaact
    12661 tctgcatgtt gatgacctct cgaggaaaaa ttaaacgcgt cagcatggaa gaattcgcgt
    12721 cagtccgtcc ctcgggcctg atcgccatgt cactggaaga aggcgaccaa ttgggatggg
    12781 cccgcctcac ttccggcaag gatgagatca tcatcgtcac cgaaaatgga caggcgctgc
    12841 gctttagcga agcaaaggtc cgtgccatgg gccgccaggc ggcgggtgtg cagggaatta
    12901 aactcaagaa agacgatgca gtcaccagca tggacgtggt cgaaaaagac ggcgctttgc
    12961 tggtcgtcac caccaggggc ttcggcaagc aaacgccgct cagtgattac gccgccaagg
    13021 gacgtgccac cagcggaaat tacacgatcg accccaaagc cctgaaagag attggcaaga
    13081 tcgccgcggc ccgcgtcgtt caaaaagcgg acgatctgac gatcatgacc tccaatggcg
    13141 tgaccctgcg tttgaaagtg aaggatgtga agcaatcggg ccgcgctacg aagggtgtcc
    13201 acctcatcaa gccgcaggat ggtgacaatg ttgcctctgt ggcacgcatc tctgcagagg
    13261 atttgaagaa agcaggcgca caggtccttg aagctgaaaa agtggaagaa cagccaaaac
    13321 tggtgtaact gcccaacgaa cctacaacat agttaaaaca aaaactgcct gaccatttgt
    13381 gtcaggcagt tttatttcag gaaatttcag aaaccgatct tgcctgtgat aaaaagagcc
    13441 ataaaaccga tcacgcacac ggcaagcatg aacatgacag aaaggatgaa acgctgctgg
    13501 cctgtcattc caagaatacg cctcgaacga ggcttggcag tgaaggttgg tgcaggtcct
    13561 ttcacctcca gatattttgg cgctggggcc tcgtcgtaac tggatgcggc atcttcacgg
    13621 agattgtcaa attcattcat ggtcacctca ttatttttaa aagtatatca tgcgtctggc
    13681 ctcaggctga catctccaaa gcggacaaca atggatttgc gatgtttatc aagcaggcgc
    13741 aggcttccat caggctccag gccaagcagt tcccctacca cgggtacatc agtccctgca
    13801 aaggtctgca cttgctgacc tcgaaatgca agttgatttt cccatgcttt gataaattca
    13861 tccgttccca ttcgggcacg ccaatgaata agtgcctcaa gaatactgga caagacttcc
    13921 tcccgattgg gcggggtgcc cagttcatcc tccaggctgg tggcagggaa ctgtaacagg
    13981 tcggcaggag ggacagatga tttgaagaca ttgatgccca ctccgatgac aagcaggtca
    14041 agttcatccc cagaccaaac ggtttccacc aaaatacccg ccagcttttc tccgtttatc
    14101 aaaatatcgt tcggccattt gatggatggg gttagtccgt ttttagagca ggattctgca
    14161 attgaaagcg ccgccagtcc cacggtccgc gagagattcg ggcgcagggt ttctgaagag
    14221 cgcaaaatca aactaaaggc aagcgcactg ccctttgggg tgaaccattt tcggttgagt
    14281 cggccgcggc cctgtatctg ttcatccgca acaaccaacg agagatccgc cgccccgcct
    14341 tcggtggcag cccaggcaag cgcctcatca ttggtggaac cgatggaatc aaaataccgc
    14401 aagccgccaa gctttaattt ggaaagtgct tttttgaggg agttttcgtt catggaatca
    14461 aaagacctga cggctttttt gaaaaaccgt caggtctgca ttgttattga acaatatcgt
    14521 aggggttgat attctggccg ccgatgcgga tttcaaagtg caaatgtgcg ccaaaggaat
    14581 taccggtatt gccagaatag ccgatgactg caccctgccc cacaaattga cacacactga
    14641 caacgtaaga gcttaagtgg gcataaaccg ttacatagcc gttaccgtgg tcgatctgca
    14701 ctacgttgcc atagccataa ttccagccgc cttgagcctg agtcaccaca cctgcccctg
    14761 ctgcatatac atttgaaccc tcgggtgcag agatatcaat gccaagatgg cttggcccat
    14821 acgggttacc agaaaggctg tggtcatcgg caggccagcc aaaaccagta gctaccgggc
    14881 cgccgccaca agcattggtg gcattgccac cacctgtacc ggtccccgct cccctgccca
    14941 tggtttgcag gtctgccgcc cagtttcgca gatcgcgcgt gccgcctgga accattaccg
    15001 ttgtgcctgg ttcgatcttg gggtcggtga ggtcaacgtc gtttccaggg aagttcacaa
    15061 tatcatcggc attggcgctg aatttctcag caaccgattc aaaagtgtca ccttcctgcc
    15121 actcgtagta caccccatcc actggcggaa tggttaactc cataccaggt ttcaggttgt
    15181 gtgggttatc ttccaattga tcgttggcaa aaagaatgga ttcagatttt agtttgaagt
    15241 cgtctgcgat cgaaagcatt gcatcaccac gcgatacacg gtacgtggta gacgcataac
    15301 gaggccgttc cgggatattc gtcttcaatt gcaatcgtcg aagaatggat aaaaccccgc
    15361 ctccacccga cccggcagcg ggtacgccca cctgaggctg accagcaact ggagcttctt
    15421 ccggggtggg ctgcacaact acgggagcgg cattggcaag ggtgctctta taaaaaaggg
    15481 tcgaacccaa cagcgacaca acgatcaaca aagtcaccgc ccaactgatg atcgtaaaac
    15541 gatccaccct gggctttgca ccttgtccac gcggtgtggt gggaggttca ttcaccgccg
    15601 acacttcctt tggcttgcgc ttaaatctgc cgccaatgga agtgaagaag gcattaattt
    15661 tctcaaaaag ggttttcatt ctttcaattg tagtacaaat cgacagttcg cgttacgcgt
    15721 cctttgtcag attctctagg aattcgatgt tgttcttggc acgttccatg cgggtgatga
    15781 tggcttccgt ggcaacggaa aggtccatcc ctgccccacc aggaggcggt gtgatcattt
    15841 gaatgaacat gcggcgcata agccagacac gctggagcag gtctgggcca agtaacaggt
    15901 cttcgcggcg cgtggacgaa cgttcgatat ccacggcggg gaaaatgcgg cgttcctgaa
    15961 gcttgcggga aaggatcaat tccatgttgc ccgttccctt gaattcttca tagaccacat
    16021 catcaaggcg ggagccggta tccacgagac aggtggcaat gatggtgagc gagccgccat
    16081 cttccacatt acgggctgcg ccaaagaaac gcttgggcgg atacaaagca gaaggatcca
    16141 taccaccaga aagggtacgg cctgagggat tcacaacaag gttatacgca cgagccaaac
    16201 gggtaatcga gtccatgaga atgaccacgt ggcggccaat ttccaccaaa cgtttggcgc
    16261 gttcgagagc cagttccgct gtacgaacgt gggcggtaac gggttcgtca aaggtggatg
    16321 caaccacttc cgcatccacg gaacgatcca tatcggttac ttcttcaggg cgctcgccaa
    16381 taagggcaat gattaagtga acgtctggat atttggttga aattgagttt gcaatttgct
    16441 tcaaaatcgt ggttttgccc gccttgggag gagaaacgat caatccgcgc tggccacgtc
    16501 caatcggtgc aatgaggttg atgagacgcg gggccagttg gtcgtgtcca atttcaagat
    16561 caaaacgctt atcggggaag atcggggtga gattttcaaa aacaacccta cgggagtttc
    16621 ctggctcagg ctcaagcccg ttgattgatt caactttgag aagaccaaaa tgcctttcac
    16681 tttcgcgggg aggacgcacc tgcccaatca ccagatcgcc tgcgcgaagg tcatattttc
    16741 tcaactgtgc ctgtgaaacg taaacatcct gatcgctgat gcggtaattg gtggcacgta
    16801 gaaagccaat accttcgctc attatttcaa gaatgccgcc gcgcagttca atgccttctt
    16861 tttgcgcctc cgcctcgcgg atcatcatgg caagggtctc ttttttcaga cgattcgcat
    16921 ttggaatatt gagcgtcttt gccatgccgc gcagtttgga tagcggttca ttctctaatt
    16981 caagtatttt catgttggtg tagtctctta tgggatatgg tttggaatgt gaaagacacc
    17041 agtccctttt taagggctgg cgaatttcct caggaaattt ttggtgtgtg tggggttttg
    17101 ctcaaggtta tcgttgcggc ttccaggcgc gaccaaatta cgtctgggtt ttgacaaaaa
    17161 tgaattgtca aatatcaagg ggagatgccg cgattatagc acttccatgc agggggtgca
    17221 agcacaataa tattgaagtc atataaacaa gccgtatgat gagatgcctt tacatctctc
    17281 atacggcttg ctatggaaga aggaaaaaat cttacgaacg tttactttgg tataccacca
    17341 tgaggatttt gatatttagc acaacaaagc gccaaagttg ataaagtttg aagttcatcc
    17401 agaatttttc tgctttggaa aattcctgtg aggtctgcat atgccttctc cttattcggg
    17461 gataatggtc cagccggggc gggctttgaa tttccagagg aaggtgtggt ggagcatgaa
    17521 tttcatccat gcgccgctga cacccatttc gagatgcgag gtgaacaggt cacggccgaa
    17581 ttcgttttgg tattttttgc ggtctggcac cacaggctgc atgacgatgg atacggcatt
    17641 gccatcccaa agagaatcgc ccatgctggc aatacaggcg gccaccattt cagtcatcgg
    17701 ctcgcgatga gtcatttcac ctttttcgat caggtcgaca atgttgaggg ctactacacg
    17761 tccgatcacc ccgctgacca tgcctgtgcg tggaggtgca ggtgcaatga gcaggttgtt
    17821 tttgttggtg aacggtttgg aaattggccc gggaggtgca aaggcgatgc ctgccgcgaa
    17881 gacgtttttg taaatgggat tctgatattg ggagggccat gcttcgggtg tttgggcaag
    17941 ctcggggtaa ggcagaccgt attttccatc cacaaggaca aaaccagcgg ggttggttat
    18001 cttcgaggtg atgtcttcgc cgtttttgtc gatgtatttg aagggctggc ctttgaactg
    18061 cggaatcatc attgagaagt caaaatcggt ggtgccttct tcgccatcat aattttgcca
    18121 gtggatcttg ccttcctcca ctttggtgac gccagtttgc acttgatatc gaatgccatg
    18181 gtcattgaaa accgaggtga ggaattcctc actgcgcatg atgtgtccat tgcgtttgat
    18241 ctgcacgcca ttcacgccaa aatcaccgat ggcgggttca ttggaaagcc agagaatatc
    18301 ggctttatca cgcaatcctt ttttgaccag atccttgtga acattgacaa tgtattcaaa
    18361 ggcggcaccc tgacaggttg ccccgggatg ccccgtaccc atcaccagtt tgaccttttc
    18421 tcccttcttc atgcggtcac acaaagcgag ataggcatca cgagttttaa tggcatgcgg
    18481 cagggaacag atggaatgtg taaaaccatt ttctggccca aggccgggtg tgcccgcaaa
    18541 attaagcagc ggcccggggg cgatgacaag ataatcgtaa tcaaggcgca cttcctgccc
    18601 agaagcaact ccttcgccca atacatattg gtcgcctgca tcggggtgaa tttcttttgc
    18661 agcggcatgc acgaacttta cattcatttt gtcgtacaca ggtttgagtt tgaaaatggt
    18721 ctgctcaggt ttcatgtgcc ccacccccac ccatacccat gacggtacat atccgaacac
    18781 atcacggttg ctgatgaccg tgatctcatg ttttttaccc aagcggctcc ccaaatacaa
    18841 cgcggctgta tgacctgcaa accctgcacc aatcacaaca acttttgcca tataccatgt
    18901 ctccttgcgt tttagtgtag tcagcgcctg caacaactcg gggagttaac aagcgtcaac
    18961 aaaaaatgcc atgatgaaat catagcattt ttgtgattca ttttttatcc cgttgacggg
    19021 tgattttttt gtcccccaca ggggtgtaaa accgagtaat ttggtgcgaa ttgttttatt
    19081 tgattccctg tgatgatttt tgccaacaca taaatttaac agccaaattt atgattgagt
    19141 ccaaaccctg tttatttggg gttggggtat gcagtttcct tgccgggggt atccaggcca
    19201 tcaatggtgc gagtgccata gagttgacca tccaccgtga tcgtgtattg acctgtgcca
    19261 gacacggtct ggtcagttcc tgcaaggctg ttccaggtga agattaattg attgccttcc
    19321 agggtacctg tcccctcttg aataccgcct gagatcagcc attgaaattt gtactcattg
    19381 gcctgtccac cttgtgtaat ggtcagggtg ccgccgtatt cttcgccggt tgggtcggtg
    19441 gcgttcaagg cataggagcc tacaatatcg ggaatattat ccgcagaggc atctgcaccc
    19501 agcggacctg gtttattacg tccgcatgca gaaagaccca atgcgatcaa caaaatcaac
    19561 aagctaaatc gtctcatttt tcctcttatc aaaacaatgg acaatgaatg aaaagcactc
    19621 attgtccatt tcaaattttt tattgtccat ccaaagtcaa caaaaaggcg atcaggtcct
    19681 ggatttcctc ttcagtgagg atcgtgccga agttttggat catctgtccc gaaggataac
    19741 cagaaaccac atacgcattc gggtcaacaa tggattggtg caggtactct tcggcagtca
    19801 acccgggtac acgctccccg gcgcggttgg caataccata aaacgacggg ccgatgatgg
    19861 tctcaccttt ttcaagcgaa tggcaaatgc ggcagcccgc gttaacccct gaagcggttt
    19921 cgtagtaaag ctgctcccct gccttggggt ctggaatgtg gacatctctg aaattgatgg
    19981 tcaacgcctg ttgaggttca agattaaccg tatcatcaaa caagatttgc tcaaaactgg
    20041 catcggcacg gtcgtacatt tttacggtga tgtggtgcct tccaacgggc aggtggatat
    20101 gctcaaaaat acgagcgcct tgattgatat ggttatcggt ggcggtgtag gtttcgtcaa
    20161 agacgagttc gtgatccact tccagcgtga tacgaatcgg ttgttcaaga tcaggctcaa
    20221 gcggaggaag aggcgtcaca tcctgaacgg ggtatccact gtgatgggtc atctgaatcg
    20281 ccagaacagc agaatcttcg ttaaagaatt ttgtcggcca gtcactcaac cagatctgga
    20341 aggcagtgac aacggccatc acgccaagca aggggatgat gaatcgaaga tgcgatgcgt
    20401 tcggtgtgaa cttgaatgta ttcggttcag acttaatctg agtcttgatc gccttaccaa
    20461 aatcagcagg cgccgcccag gcagtgtgaa ccagcggaat aaagttctgt ttgagttttg
    20521 gcagtctttc gccagagaca cgatcattca accagacgtt gccttcacgg ttggcacaat
    20581 cttcgggcgg acaaccgatg aattgaacat cgcttgcacc cgcttcgagt gcctgtgcag
    20641 ccaggttggg attcgccatg gcaatacaag tcagcgggac aatgtatgta tttgggtctt
    20701 caaaatgctt ccctgcacca tgcagggcat ggcgttcgca ggtgaagaca acttttacct
    20761 tgctcttgtc atcttgagcg attcgctcga gggttgtttt ccaaagtggg ttgaggggaa
    20821 tatcgccaaa ggtcagggcg ttatcggggc agctaccaat acagacgccg caggaaacgc
    20881 aaagattcgg gtcgatctcc gcctgaaatt ttggcctggc accatcggta cggggaacca
    20941 tcttaatggc aaggtaggga cagtcacgtt cgcacagggt gcagccatca caattggcca
    21001 aatccacttt tacaggttcg agttctttgg cacgcggcag gatccacggc aaagcagtaa
    21061 cgaggccaac aatgaaaagt agaacactcc agaagagggc ttgaggcccg cgcaggaagg
    21121 ttgaaagata gaataaatag aataagtcaa cgggggcttc ggcaggaagt tgcgtggcat
    21181 taagcggcgg caacatacca aggggaaaaa gcgcagccga gagcaagagt acaaaaaccg
    21241 aaatcgccat ccaatatcgt ggaggcagcc actttgcgcg gctcattcgt ttgaggtgca
    21301 gccagaggaa gaaggcagtc agcgcagata ggcctaaatg cagcacgatg acgatcatca
    21361 tgaagaccca gcctgtacct gccgcgtcgc cgacaatgaa atcaacgatg aaggtctgtc
    21421 cgtttttgaa actgccaagg atgctgaaaa gactttggtt caggagctgg gcacgttcat
    21481 cccagatcag ccagtagcct gtgatgccaa tggcccaaac caccactgcc ataccaacac
    21541 cagtaaccca tgccagccag cgcggtccgc ggaaacgatc ctgaaaaaag gtccgccagc
    21601 cgtgcaggag ggcaaaaatc accgcagcat cagatgcata acgatgcatg gcgcggatga
    21661 tgcgtccgat caggttcgcc tcgattttgg atatcgcctg atacgagaag gtgaatccaa
    21721 acgggtagaa catcgtcagg tagatgcccg taagcagaat aaccaccaaa agaaaaatgg
    21781 tgatggtgcc tgtatgatac agcggattga agcgcgggtc attgatgagc cagttgacgg
    21841 gtgcttcaag gcgcaaagaa atgctttcga aaaaattgag caggcggcgg ctgggtgaat
    21901 tgaggcgtgc tttccaatcc acgctggggt tgcgctcggt gagaacatcg atgagttgtt
    21961 ttttagtcgg ggaggtagcc atgtgttttg ctgacagttt cgtttacaaa taatttcgcg
    22021 gaagtaatag tggataaaag cagacgtcaa tcaataaagt ttgcgaaatg aaacagacaa
    22081 gaagaaagtg acagtcacct tgcgggaaag gtgactgtca ctttcgtttt caaattacca
    22141 aactctgaag cctggggagg tcagggcgat gttcttgatc atttcaaaga ggacagggcc
    22201 gtacataatg acgatcagtc cgagcgcacc ataaatccac ggagcccagc ggtcaagcca
    22261 caccggggtg cgagacacct cttgcatgga ttcagcaatg gggacaatca cctcttcgtt
    22321 aaccttttcc ttgtttaagg cagtcatgag cataaccgtg agatagattg caccgctgac
    22381 aaagaggatg gccccaccca cacccacctg gaagaggcgg ctgttccatt cagcaggaac
    22441 gtagggagcc aaaccaagtg gtacgcggcg gggagcgccg agcaaaccca acacatgcat
    22501 ggagttggag aagatgatca taccaacaaa ccatgtccat gcctgagcaa ctgcccattt
    22561 ggggttccat aactttctgc cagtgagata tggcagaagc cagtaggaaa tgcccatgaa
    22621 gctgagggtt acagcagtag ccaccgtcag gtggaagtga ccggggataa aagcagtgtt
    22681 gtgcaacaca aggttgacat tgtaggaggc atttgtcaaa ccaccgatgc cgccaaagaa
    22741 gaacaggatg ccagccaaaa gctgtgtagc tacggatggg tcgctccagt tcagttttcc
    22801 aatccagcca aacattccta agccaccttg tttgcgcccg gcatgttcaa gggaagctac
    22861 cacagtgaag gcggtcatca tggatgggaa aaatacgcca aatgtaaaca tggagtgcag
    22921 gaatttccaa acaggagaaa cgcccgggtc tacatattga tggtgaatac cgataggggt
    22981 ggagaggacg aggaagatcc agaaggcaag gcgggcaagt gaatcgctga acatcttgcc
    23041 attgccagcc tgtttcggaa gcatggcata ccaggagata taggcaggca gaagccagaa
    23101 gtacaccaat ggatggccag tgaaccagaa ataggtgcgg gccaattgcg ggtctacacc
    23161 ttcaatcaag ccaagtgacc atggcagaag catggtgagg atttccacag caacgccgag
    23221 ggtacaaatc tgccaaagca gggctgttac aacactggca aaagcaatga aaggtgaacg
    23281 ttcatcgggg ttttccttgc gccatgcggc aaaagtcata aacaaacccc agccttccac
    23341 ccaactgccg accacaacaa gggtcaaccc tacatagaaa gcccagttgg ctttgagagg
    23401 aggatagaag gtaaagagca cagtggcttg attggccaaa atcggaatcg cagcagcgac
    23461 aacgccgacc accatcatcc aaaaaccaac cttgttcagg gccatatttt tgagagggcg
    23521 tttcaggcca aaaatcacat tgaaagtgag aaagcccatg atgaagaagg tcgtccaaac
    23581 cagggcgttc aaaacgccgt ggacggtaag tccctgatag taggacttaa tgaaaggctt
    23641 gagataaaca tagaaatccc agcccgcatg ttcaaaagcc tgcagagggc caaataaact
    23701 gcccacagca agcgccaaaa tggccactaa aaggtgtgag ccaatgaatt tcttttcacc
    23761 agcagataat tgatattgtt tcatgatgtc tctcttcaca gctattctgt aacaatgatc
    23821 gcgccataca tcacaccgtg accgctgcca cagtattcgg tacaaatgaa gttataggta
    23881 ccaggcttgt caaatgtgat ggtcagtttt gatacctggc caggaataac catgaaattt
    23941 gcgtttgtat cctgaatctt gaagccatgc tgaacatcag atgaagttac atagaaggat
    24001 acactggagc ctgcgggtac ggtcagttcc ttgggcagat attcccagct ggggatcgca
    24061 cgagccaaaa tgtaggcatc atacttgccg ggttcgattt cacgcaaacc ggggttcgac
    24121 caggggctgt tggggtcggt tgccacagtg cggggatcaa cacgctgttc cggggcaggc
    24181 acctgaattc ccataccgaa ggcagcaaaa ctgacagccg ctgcaaacaa aacgagcagg
    24241 atggcgctga cgatcatcca gttacgctca taggaatcaa tgtgaatctg gttggtgctc
    24301 ataatgtgcc tcctctgccg acaaggatca tgtagatcgt tgcccagatt gcgatgagtg
    24361 tgactacaaa gatcgccaaa actgcgatcg tgccagtcgg gcgaaactca tcgtcttctt
    24421 tatctttttt cgccatgttt cattctcctc ttaaaaatga tggattagga taactaccgc
    24481 ttcatgcggc gccgcaaact gcggaagtgg aaatggggtt gatcttacgc tgttgcagtt
    24541 ccacctccct attgctttag catgtacttc aaatcatcgg cgatctcatc agcagtcacg
    24601 ccgaatggaa ataccaattt caaatatccc tcacggtcta tcaccatgag agtggcggtg
    24661 tgatcgatca agtaaccact tgccgcactg ccttccttct tctcatagaa aattccatac
    24721 agagaggcga cagtatccac ctgctcttgt gtaccggtaa tacctaaaaa ggtggggttg
    24781 aaatgagcga catattcttc caattttgcg ggagtatcgc gctcggggtc cagcgagatc
    24841 ataatcaact gaatgtcttc tgccttgctg ccaagttttt tcatggcctg attgacttgt
    24901 cccagtgtgg cggggcaaac atcggggcag aaggtgtagc caaaataaat cagaaccaac
    24961 ttgccgcgga aatcacttag cgaaacatcg cctttgccgc ccgtcaggtt gaaatcatag
    25021 gatgtttcag gagactggat caccgtgcca tgaaaagtat gcggacggaa aaaataaact
    25081 cctgttacca ggcctaaaat aatggcgaat gtaccgagca gtatctttgt tttcataatt
    25141 caaaatattt tatggagcat agcagaggaa taaatgagcc gcttcataag ccagcttggt
    25201 ggttccctta ctgttgcgaa cctcttctgc taacacgcca aggtgacgga ggatgcggtc
    25261 tgcattggaa acttcggtgg cgtagcgata ctctgcgcgg acaatgcccc agccatccac
    25321 cagcacaaag gatgggtcaa aggcaaaact accatcttcc tttttttcgt aataggtttc
    25381 aaagcccgaa ccaatgatgg tcttgagcat tgcttcgttg gttgtggttg caaatttcca
    25441 tcgatttgta tctgcgccaa tggattgggc gtaggcattc agcgcgtcag gggtgtcacg
    25501 ctccgggtcc atggagatgg tgacgaaggc tacggaaata ccgtccaatt ccacttcgcc
    25561 gaggcgggat tgcacttcaa gcatggtgtc gttgatgctg ttacaaacct ctggacaatt
    25621 ggtgtattcg aatgtgtaaa gtacgaactg tccacgcagg tcttcgctgg tgagacggtt
    25681 actgtcttgg tctgtgaggc tgaaagcagg tgaaagctgc atacgcggca ggactttaat
    25741 cggttgaaag accttaaaag caaaagcgga aacaacaatc aagccaaaca agccataaaa
    25801 actgccgagg aaatacttgt tcatgcaggg atttcctcgg ggagaaggat tttgagcgga
    25861 gcagaatcag gtttcggtgc ggatgaagcg ttctttatca gattggcttc gatttgttgg
    25921 ccttcatcca ccaattgttg gaaggtcgtc tgtgccattt cattccgcag catattcttt
    25981 agcctcaccc attgacagtg gacagggcag cgcagttcga acgggcaatc accggttttg
    26041 agaacacaat cggaaagata aagttctcct tcaaaatgct cgacaatttg aagcagggtg
    26101 atctgtttgg gagaatgagc cagagtaatg ccgccatcgc gcccggcctg ggtaagaata
    26161 aaaccgccgc ttgccagttc tgccacaatg cgttgaagca aggcgggggg aataagcatt
    26221 tcacgcccaa cctctgaagt ggaaacgcgc ttgccctctt cctttttgga gagggcaagt
    26281 attactcgaa ctgcgtaatc ggtttggcgg ttgattcgga gcataacgtt tccagttcaa
    26341 tattgagtgc aagagtcaat gtttagggct attgtaagaa tttcttagtt ttttttgacc
    26401 tgccattaat cacatcggca catgacaatc atcccatctt ttgtggcggt ctgtacagta
    26461 tttattacac attttttatc tgaattatcg caactgcgtt gagaaaatcc ctaccaactt
    26521 tgaatgatat aatcttttca aaaataaatc gtgaggtagt agaaagaaat ggatcctgta
    26581 atttttcaat tcaaattgtt tggcggagaa attgttctgc gctggtatgg tgtgttggtt
    26641 atgcttgggg ctgtggtcgg tgcatggctg gcagagaagg aaatcaccag gcgcggccag
    26701 aacggtgaag tgatctggga tgcaatggtt tgggtattac ctgcgggcat cattggtgcg
    26761 cgtttgtggt acgtactcaa cagcattctc ggcggaagtt cgtattacac agataatccg
    26821 ggaaagattt tgcaagtatg ggaaggcggc ctgcatttct ttggcggcct gctctttggt
    26881 gcattggcct tgtatttctt tttgaaagcc aatggattgg atttctggct ctttttggat
    26941 gcaattgcac cggccgtatt catcggtcag gctttggccc ggcctgccaa ctttatcaat
    27001 caagagttat acggccagcc gacaaagctg ccgtggggca tttcgatcga tgcttctcat
    27061 cgccttgctc aatatgctga cctcagccag ttccctgttg agacaacccg cttccacccg
    27121 acgtttgcct acgagatgat tctgaatatt cttctggcat tgttattgct ttgggtggca
    27181 cgtcaatacg aagaaaagtt gaaaccaggt gcgatctttg cgggctggct tgtgcttgcc
    27241 ggtttggctc gggcatttat cgaattcttc cgccctgacc agccgcacat cggggattca
    27301 tttgtcacat tcaccatgtt ggtttcattg ttaatggcgg ttgcaggtgc agtcatgttg
    27361 atggtgcgtt atggccgctt gcaactggct tttgccgcca actgggaaga ggaatatcaa
    27421 cttaagaagg ttgaggccgc ccccgccccc aaacgcaaaa cttctgttgc atccactgca
    27481 aaagagagtg acgaagaaac agaaattgaa atacaggacg aagatgagga aatggaagaa
    27541 acccctgctc ctgtaaaaaa gagaaaaacg gctgcagcaa ccacatcaac ttctacaacg
    27601 aaaaagaaaa gcacgacaaa gtcaaaaaca tccaaataag taactctgtc tcacccgcga
    27661 aagctcgttc tcgcgggtga gttcattctt acaggtcaaa ccaagctcat cttcccaccc
    27721 tgcataaatc ttggcatgat aaaatcgcct gcatgaatcg tctattcgct tgcttttccc
    27781 tcgtcattct ctttacttca gcctgcaccg tagctgcacc atccggcact gcaacgcagg
    27841 accaggccgt gccatctgga acagtcctct ttcaagatga ttttgccaac ctaggctcag
    27901 gttggagtcg tttttctgcc gccgaaggtg tgatggatta tgacagcggt agtttccgca
    27961 tattggtcaa tgcgttggat acgaacttct ggtcgacacc acaaaagaac tttaccgatg
    28021 tccgccttga agtggacgct ggcaaattgg gcggtcccga tgaaaaccgc atcggcttga
    28081 tctgccgctc ttccggtgcg gattactact tcttcatcat caccagcgat ggatattatg
    28141 gagtcggcct tttttcgggc ggacaggctg ttttgctcgg ccaaaatgag atgctgacga
    28201 gcgaaaacat caacaaaggg ctggcggtca accatttgcg cgccgattgt acaggtgacc
    28261 aactcacttt ttacgtcaat ggattccaga tcgctgctgt gcaggatgca actttgaaat
    28321 caggtgatgt aggcatgttg gtgggcactt ttgccaaacc cggtgtggat gtgatcctcg
    28381 ataattttgt ggcattgaaa ccataaaaac ggtctggacg aagtttgcct cccaaacctc
    28441 aagaaaaact ttccttgagg tttgaagaac cgaaatcggg ataagtgtca ctttttgaaa
    28501 agggggagaa aatgtataat actcgtgcaa tctttcacag taaatttgca aaggagcaaa
    28561 aaatgacagc tcaaatcatt gatggcaccg ccatcgcgca gaaggttcgt gatgaagtta
    28621 aagagaaagt cgctgcaagg atcgcggcgg gaaagtcaca acctggactg gccactgtat
    28681 tggtcggtga ccgcgttgac tctgccgcct acgtgggcat gaaacagaag gcctgcgctg
    28741 aacttggcat gacttcctat catcacccac tgcctgccga tgtatcacag gaagaccttg
    28801 agaaactgat caaggaatta aacgccgacc cgaaggtcaa cgggattttg gtgcaactgc
    28861 ctctgccctc tcaccttgat gaagagcgcg ttctgcagct catcagcatc gaaaaggatg
    28921 tagatggctt ctcgccgatc aatcttggcc gccttgccca aaaaggacgc gaaccgcttt
    28981 ttgttccctg cacaccttat ggctgtattt atcttttgaa agaagcaggc gtaaccatca
    29041 agggcgccaa tgctgtagta ttgggtcgtt caaacatcgt cggtatgcct gcagcgttgc
    29101 tgcttgtaaa agaagatgcc acagtaacgg tcgttcactc gcgcacaaaa gatattccgg
    29161 gggtgttgcg ccaggcagat atcgtcattg cggcgatcgg ccgtgcagaa atggtccgtg
    29221 gtgactggtt gaaacccggt gcagccgtca ttgatgtggg catcaatggc attccgatgc
    29281 tcaaccccga tggcaccccc gtcatcagcc agaagaccgg caaaccgcgc cagaaactcg
    29341 tgggcgatgt gaattacgaa gaagccaaag aagtggcagg cttcatcacc ccggtgccgg
    29401 gcggtgttgg cccaatgacc attgccatgt tgatgaacaa cacattgcgc gcagcggaaa
    29461 ttcaacaacc gtaaaacgaa agacacaaaa gagaaaaaca aaagtcccgc tgcgaggcgg
    29521 gacttatttt gtaaatttgt aaagaggaga gcagaatgaa aaagtttttg aagtgggtgg
    29581 gaattgtgtt ggggatattg gcagggctgg ttgccatcgg gttggttatc atttatgtgc
    29641 aaagcgaatc aaggctgaca cacgtgtatg aaattccagc agaacaagtt gtcattccaa
    29701 ccgacgcggc ttccattgaa aatggaaagc acatttttca atttcgggga tgcgaagcct
    29761 gtcacggcga aaacctggag ggcacggttt atttggatga tcctgccgtg gggagagtca
    29821 tctcttcgaa tctcacatca ggcttgggcg gggtgggaag cgcaatgtct gattctgact
    29881 gggtgcgcgc aattcgtcac gggatccgcc caaatggcac cgcctatctc ttcatgccat
    29941 ccactgaatt ttattatttg agcgatgatg atcttgggga cgtgatcgcc tatatcaaga
    30001 gcgttgcacc agttgataat gacctgcccg ccagcgaaat ctctttcacc gggcggatcg
    30061 tgatgactct ggtgaaagac attaccttta tccccgcgga actgatccca cacgactcag
    30121 cgcgtccgac cgcgccgaaa gcgggtacaa cacccgaata cggggcctac ctgacgcagt
    30181 cctgccacgt ctgccatgga ctaaacatgt cgggcggcgt gattcccggc ttcccatcca
    30241 cctggccgcc cgccccaaac atcacttttg gcggcggttc cgccctgccc acatggacgg
    30301 aggaagagtt catgaacacc attcgcaccg gcaaaacacc gcaaggcaag gaattgcgcg
    30361 ccgcctacat gccgtggaat tcatacaaat tcatgagcga tgatgagttg aaagcggtct
    30421 gggcctattt gcaatcactg ccaaggcttg agtatgggaa caggtaaata atggtcaggc
    30481 atcaatcccg cttttggcgg gattgattat ttaaaacggt accatggaac tataaaaagc
    30541 ccttgacgta atatcaatct catactatta tctatttatt agtagtaaaa atctgatact
    30601 actgaaagga gcacaaatga ccgaaatttc gtcgcatacc aggaagtttc aaaaagaact
    30661 gaatgcaggc acttcgtcgc ttgtcctgct cagtgttttg agtcgcagca aagaacccat
    30721 gtatggatac cagatcgcaa aaatgctcga ggaaagcggc cctgagatcc ccatgatgaa
    30781 acagggcact ttgtaccctg tgttgcgttc ccttgaagag aacggtttgt tggaaagcac
    30841 cgtggaaccc tcggtttcag gcccgccacg caggtattac aagatcacca atgatggacg
    30901 ggaagccctc aaagaatggg ctgaaatctg gaagatcacg aaaaaattcg tcgacgcgaa
    30961 tttgaaagga tgacgtcatg tttaaaacga tggaagaata tcttgatgcc ttgaaaaccg
    31021 aaatgaaaga tgccgaccct gccctcgtgc aggacgcaca ggctgatgca cgtgaacacc
    31081 tgagtaccgc acttgaagca gcacgtgaag ccaaccctgc agtcagtgaa gcggatgccc
    31141 tcgggaagat catcgaagaa tacggtactc ctgaagagac cgcgtctgcc tatcgagagg
    31201 tggaaaggcg cacctcccct gcactaaaac aaaccaccaa accccgttca gcactgggcc
    31261 gattttttgg tgtgtacacc gacccgcgtg cctggggcgc cttgctttac atgttgatcg
    31321 cttttgtgac cggtgttttt tactttagct gggcagtcac cggattatca gtgtctattt
    31381 cctttttgat ctttatcttc gggtttccga ttgcgctgct gtttctgctc tcagtgcgag
    31441 ggctggcctt gctcgaaggc cgccttgtgg aagccctgct tggcatccgt atgccgcgtc
    31501 gtccgctatt ctcgcatcag ggtatgaagt ggttcgaccg cctgaaagcc ctcctcaccg
    31561 acaaagccac ctggctcatg ctggtttaca tgatcgtcca atttgttctt ggaactgcat
    31621 acttcatcct aattgtcatg gtgttaagca tctcactttc cttcatcgca ctcccgttca
    31681 tacaggaagt tctgcatcaa ggtgctgttt ataacaacgg cgttcgatat tactttccaa
    31741 gctggtccta tccgctattg gtactgggcg gactcctgct gtggacaacc ttcatgaaca
    31801 tcatccgcgg agttggtcaa ttgcatgggc gccttgccaa atggctattg gtcagtgagt
    31861 agggaaatga aacaaaactc atccgcctct ggaaaattca tgaagttcct gcaattcccg
    31921 gttacccgct tcttccttgg attctttctc atcgggctgg tcacctccat cgcgcaaatt
    31981 ggcacagaag tatggcccgg ccatgtgaac aatctggcac agatcgcatc ggtattgatc
    32041 attgtcacca ttgcaagtct tgcatattat ggatttgtcc gcgtaatcga aaaacgcgct
    32101 gtaacagaac tctcccccca tcaagccgtg cgtgaattgg caggcggagc cgcactgggc
    32161 gcattgctct tttcagcaac catcggcatt ttatggctgt ttggctttta taaagtatca
    32221 ggcaccaatg catggacagt gctcatcccc ggattggtgc tttcgatctc ctctggtgtg
    32281 atcgaagaaa tactgattcg cggcctgctc tttcgcatca tggaagaatc actcggaacc
    32341 tggatcgcgc ttgcaatctc cgccgtcatc tttgggtttt tacatttagc caatcccaat
    32401 gccactttgt ggggtgcgat cgccattgcc attgaggctg gtatcatgct ggcggcagcc
    32461 tatgtcttta cccgccgttt gtggctgccc atcggcatcc actttgcatg gaatttttcg
    32521 cagggtgcca tcttcggtgt agccgtttca ggaaatgaag tacaaggctt gttgcaatcc
    32581 acattgagcg gaccaaccct cctctccggt ggcgcgttcg gtgcagaagc ctccatcatt
    32641 gccgtaatga tctgtctgct ggcaggcatt gcactgacta cactcgcaat gaaaaaagga
    32701 aatttcattc aacccttttg ggcaaaacac aagcctgaac tcaaacaaga aaacgccacc
    32761 cccgcctgat annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggtccattt
    32881 tctaaaaccc agcccgcttc gagtagaatt tccaattcac caacaaaacg aatggaggta
    32941 gcttttatga atccatacct ggcagcggca caggctggca aaaacgattg gtggagatat
    33001 ttgatctcct ttttcaccat cctcaccatt tggttcgtga tcggctccat ccccatcatt
    33061 ttacttttgg gctatgtcat gatggatggc aaccccgcca caaacttcac acaaagcggc
    33121 ttcacaggca tctctgtcct gctggatttc tccgccacca tgctgacatt tttcccattc
    33181 atcgcagcaa ccatcttttc cattcgtgtc atccatgcca gaccattaag aacactgatc
    33241 acttccgaaa caaacgtccg ctggcacaga atcctgacgg gcgccgccgc atggtttggt
    33301 gtggccgcac tcatttccat caccgaagcc ctgctttacc ccgggcgata cgtcttcagc
    33361 ttacagcccc tgcttctgct ggtttacacc ctgctggcgc tcattctcat tcccatccaa
    33421 acttcagccg aagagttttt ctttcgcggt tatcttttgc aatggatcgg attgaagctc
    33481 aaaaacaaat ggctgctggc attcatcaac ggcgttctgt ttttcattcc gcacatggtc
    33541 aaccccgaaa tggccgtcaa cggaatcctg atgggattga attattttgc attcggcttc
    33601 ttcgcctgcc tcatcaccct gcaagacaac ggcatggaac ttgcccttgg cctgcacgca
    33661 gccaacaacc ttttcggtgc cctgtttgcc aactacaccg tcactgccct gcccagccca
    33721 gccttgttca cggtccaaac ccttgaccct gtttatggtc tcatctccac cacgatcgga
    33781 atgatcgttt tttattggat cgtattccgt aaacagccac aacccgccgt cattttggaa
    33841 taaggacagg aacgcatcaa accgacctgt cacctgccca gcttccaaaa acacaatcaa
    33901 cactgtgaaa gtgtcaaatc tcacttgaca gtcttcaccc attttgttat attctgcgaa
    33961 ttcttgttca aaataacata atataacaaa catgacatcg accattgacc gcgaaaaaaa
    34021 gattctggaa caccttcaaa aacagggaag tgcctccata caggaactag tggaggtctt
    34081 caatgtttcc aacatgacca ttcaccgtga attgaacaaa ctggcagaag caggtttgat
    34141 tcaaaaaaaa catgggggcg ttgcactggt cacccaatca cccactgcgg gtatcaatca
    34201 atgcatcatg tgcaacaaaa ccatctcaga gcggacggtc ttcatcgtac gtaccaccag
    34261 cggggaacaa aaacgtgcct gctgtgccca ttgcgggctg atggttcaaa gccagaccca
    34321 ggacgccgct caatctctca ccgccgatta cctgcacgga catatcatca gcaccaccca
    34381 ggctgtgtat gttcttggca gcgatctaaa ggtctgttgc gtaccgagtg tgttgagctt
    34441 cggctcacag gaagatgcag aaaaatttca aaagggcttt ggcggcaccc ttgcaaccat
    34501 gaaggatgcc attcaattta ttcatggaat gatgcacgcg caccaataga acgcgtgatt
    34561 caggcaaatc aaactaaaaa ggaagagata aatcatgaaa cgaattttga tgatgttggt
    34621 tttggtgggg acgctgctct tgagcgcctg tggttctaca atgggcgatg agcatgcgga
    34681 tggaacagac atcgaggcgc acgatttttg ggtgcgttca gctttgaaag atggaaacag
    34741 cgccgcttac atgctcctgc acaaccacac cgaagccgat gatgaattgg tcggagcttc
    34801 ttctgatgcg gcagcttctg ttgaaatcca cctgagccag atgaaatccg acggcaccat
    34861 ggaaatgatc ccgcaggaag caattcccct gcccgccgaa ggtgaattgg aactcaaacc
    34921 cggcagctac catattatgc tcatcggtct aaccaaagat cttaatgttg gtgatgagat
    34981 cacgctgacc ttgcatttca agaaccacga agatatcacc ctgaccattc cagtgatgga
    35041 agcccaggac atgggcggct ctggtatgga tggtcacatg ccatagtttg tgaattcgca
    35101 ccatcgccgg tgcattctcc ttttacgccc cgctatcagc agcggggcgt attttttatc
    35161 tctcggggag aaaaaggctt cttatataga aacagtttcc agttaaagga agaggcgggt
    35221 gtcacgtcag aatagggatg tatatcactg ggtaaatcta actatttaga gtaaatttat
    35281 ctaaataaag caggaaaatt catctttctt tatgtaacaa aagttacaaa caaatttcag
    35341 aagccagttt atattaagcg cacgttaatc ttttctgagg ataggctatg tcaaaaaccc
    35401 cattttttat tgccattgcg gccacggtcc ttgcgttcgg gtattttgcg tttgtgactg
    35461 atgccgcctc ttacgggggg agcgcccccg aaacctgtgc aaattgccat gtgatggatg
    35521 cacagtatga aaactggtat cacggcgcac atgagaattt cgccaaatgc accgattgcc
    35581 acctgcccca cgataatgtg gtgacctact atgccgagaa gggcagacag ggtgcaaagg
    35641 atgtgtttgc ctttgtaacc gggaatatcc ctgtgcaaat tcgcgccagc gaaaagacca
    35701 aaggcattat tcaggcgaat tgcgttcatt gccacgaaga taccgtggaa gttgttgtaa
    35761 tgaatgccca accctttgac cgctattgct gggattgtca ccgcagtgtg gcgcacggtg
    35821 accgcggcgc ttccgccatc cccttccagg attcaacact ctacccaatc aagtaaggag
    35881 aatttttatg aaaaattaca ccaaccttat tcttgcagga cttgtgatcg ttttggctgc
    35941 cgcactcgtg ggcgtactga cctttgtgaa aaatcagccc gcgcaggaga gagcaacaga
    36001 accattggtt gagattgccg ccatggaacc tgattcggcc aaatggggtg tgaatttccc
    36061 caatcaatac gactcattca tgaagaccca gacgaacaat atcgacacaa cctatgcagg
    36121 gtcttcccag ttctcgtggc tggaacgcga tccgcgtcag gtgattttgt ttgccggtta
    36181 tccatttagc aaggattaca acgacgaccg tggtcacgcg aatgcgttgg aagatgtacg
    36241 tgcaacgggt cgtttgaacc ttgacgactc aaatcccaag cacacacctg ccacatgcta
    36301 ctcttgcaaa tcaaccgata accctggttt gtgggcagag atgggtatgg aagcctatga
    36361 caagatgtcc ttcaatgaaa tgaccccgaa catcaacaat gccattggct gtgccaactg
    36421 ccacgaagca gagaccatgc gcctgatcgt gaccaacccc gcactggaag aagcctttga
    36481 acgccagggc aaggattgga ccaccttcac ccgtcaggaa atgcggaccg tagtctgtgc
    36541 aaactgccat gttgaatatt atttcaaggg tgatggcaag tatctcacct tcccatggga
    36601 caacggcaca aaagtggatg aaatgctggc ctattacgaa gaagcaggtt tcaaggattg
    36661 ggaataccct gacaccggaa cccccatgct caaagcccag caccctgaat acgaattctt
    36721 caccgctgat agcacccact acaaggctgg cgtttcctgt gccgactgcc atatgccgta
    36781 cacccgcgat ggcgccgcaa aatactcttc gcatgatgtc cacagcccct tgctcaatgc
    36841 ggaagaagcc tgcggtcagt gccacacaga tacggaattc gtcgtcaatc gtgtaaatac
    36901 cattcaggaa caggttgcca cgacaaagat caccactgaa gacgcaattc ttgatgcagt
    36961 caatgccatc aaggccgcgg ttgcggctgg caatgccgac cctgccctgc tggatcaggc
    37021 ccgcaaactg caccgtgaag cccaatacat gtgggatatt gtctcggctg aaaacagcac
    37081 gggcttccac aatcccgaat acgcgttgaa agtccttgcc gaagcgacca accgtgcccg
    37141 ccaggcccaa atgctcgccg cccagtccat caacgacacc agtctgttgg caacaggcac
    37201 ttactacgca acacccgccc cataaatcag gtccaatcaa ctcagttccc cgtcagcaat
    37261 ggcggggaac ttttttttct gggaacatct gcgttgaaaa tatcgtcaca gatgcaagga
    37321 gaaaaaatga agaaacttat tttattgttg ctggcactca tcctcacagc ctgctctgca
    37381 gggaccctca atgagtttga taaaaacctc gccaaatggg aatccgcaaa gatcacgcat
    37441 tatcgctacg acgtcgccat cggttgtttc tgcgcattca gagatgacat gcccatgacc
    37501 attgaaatca acaatggcga gatcgtttcc attacttcgg tcaagggaaa catcatcact
    37561 ccgtctgatg gcttgtatga cgtggtaaaa agctatgcag gcatggaagc cctgttcgag
    37621 caattgaaag gcgcatttga agatgcagat cgggtcgaag tgatatacga caccgtcaac
    37681 gggtatccca cttccattgc gattgaccag atcgaggcgg cggtggacga cgagttatac
    37741 atcagtgttg aaaatttcga agtcttgaaa taacagtcca tttttgaatg caaataaaaa
    37801 agcggcccgc aaaggccgct tttgattcaa atcactttat tccatccatt gactgaaaat
    37861 gttttgatcg acgatctgga tgcgcctgtc ttccaccttg atcgcgccgc tgcgttcaag
    37921 ttccctcatg gaacgcgcta caacctcgcg cacggttccc agcctcgccg caagttgttc
    37981 ctgagtccaa tgcggcgcgg acctctcctg gggcatttcc gcgatcaaac gcgccagacg
    38041 atgagtcacc tgatagaaag ccatctcgct gaccatgcgc accatcccgc gcagcattcg
    38101 cccaaagttt gccagcacct tctgcgcaaa atgcggatgc aattccacga gcctgcgcag
    38161 caattcgcca tcgatcaccc acacacggca ggcttccagc gcctctacat ttacagggtt
    38221 tgccgcgcca tcaaaagcag gcacttctgc aaaagtatcg ccctcctgca aaatgcggac
    38281 aatgtattga cgtccctgcg gcgaaaggcg gaagatcttc gcgctcccct gctccacaat
    38341 gtacaaacca tcacacgggt caccttccca aaaaagcaca tcgccgcgct ggtattctcg
    38401 caaatgcata tgctcagaaa tgtccttcaa catgggttca ggaaggttgt cgaagtattc
    38461 atttcccttc aaggttttta tacggttttc gtgacttact tcgatcgttt tcatggtggc
    38521 atcaaagctc gccaagcagc actttgtcgg cgccgcgatc cacttcccat ggatagacaa
    38581 tgtaagcatc agtgactgcc gcgtaataat cggggcgggc attgccaaac aggttgcggt
    38641 atggattgaa atgcagcaca caggtttcag caaacccacc cgccgcagac acacgattct
    38701 tcaccgctgt gatggttcgt ccggagcccc aaacatcatc cacgatcagg gtggggcgtc
    38761 cgcgcagttt atcttcagca ggaaactgaa tgaactccgg ccacaccatc aggttggatt
    38821 tctctgtttt ggattgcgcg gggaaatcca cagcggcagt gaggatgtga gtaatatcca
    38881 tcgcatcggc aagcaggccg ccggggatga ttccaccacg ggtgatcatc accatcgcgg
    38941 taaattcacg tttgaactga ggcagcaggt ggtcaatgag gcggtcaatc tcctgccagg
    39001 tgaccatttc tcggcggggt tcaggttgca tgggatcgaa ttcttctttc ttctttcatc
    39061 tgtcctcttt cctccattac aaaaaagagg acagaaaata aattaagcca gcgccgcagc
    39121 caacggagca ggagcgcgga cttgttcagc gctgccgtgc gcacgggcgg tttcaacctg
    39181 gcgcgcccag atgtacaggc aggtgtgata agcggtattg gtgtaggagc taaagaggtt
    39241 ggccagcatc acaaaggtaa agaagatcaa cgcaccgatg acaatgccga tgatggagat
    39301 tgccgtgctg ccaccggaaa cgaatgccgc gccgccgccg atgataaaag caatgacaag
    39361 accaatggca ccaaacacaa agccgatcaa gccagtcacc caacgcacac ccaccgtact
    39421 gatacccacc agcagaaggt tttccttggt gatcttcaaa atgcgttgca tcccgtcttt
    39481 gagattgaga tcgtcaatga tcatggcagg caagatcaac aaagctgctt ccgtccacaa
    39541 agtttcgagc aggcctgtgg ctgcgcgtgc cacactggcg gccacgccgc ccctgcggtt
    39601 gcgattggcc aggctgcgca acagattcac agccgtggat gcggctgcaa gggtgagaat
    39661 atcgaagaaa tcacgacgga cgatcgccca ggccttgccc atgtcgccat caccttcggt
    39721 gaggtagcca tagatcaaat gcacggtcat gcctgaaaag acataagtga ccacaaattg
    39781 aacaaagacc aaaataccac ccaacacgaa caaaatgata tttccgatct gggtatcgcc
    39841 aaagagaatg gccgccccaa caatgggaat gatcccaatg accgaaacga tcatgcccac
    39901 aataagcgca tagatcgaag gtttgagcag gtccttatct ttgaacgcca tctcccaggc
    39961 ctgtttgaga aaggcccagc ctcgtgaaaa actttgcata gttcactctc ctttttaatg
    40021 ttgagttgat tatagcaaag atgaggaaag agaaaagagg aaagatgcat gtataatcgg
    40081 gcaattcaaa tctcaattca ggacaaatat gaaaaagact attctgcttt tgatcgcgct
    40141 tgcgctatca gcttgttcag ccatggcaaa agccgagccc acccccaccc cagacccgct
    40201 tgtattgcag ggacagcagg tcttcaacgc caaatgcgcc acctgccacg cccttgtccc
    40261 tgataccatc atcatcgggc cgtcgctgaa tggaattgcc acccgtgccg aaacccgcgt
    40321 ggaaggggtc aacgccgagg aatatatcca gctatccatt cttcggcctg gtgactatgt
    40381 ggtggaaggt ttcaacaacg tgatgatcac caactttgcc aaggaactca ccaacgaaga
    40441 tatgaatgcg ctggtcgcat ttttattgac actgaaataa aaagccaaaa gaggagaatg
    40501 tatgaaagtt tggatgaaac gtattttttc agggttgggc attttgctgc tcatcggtat
    40561 tttgtatgcc gcattcgcac cgctgccgca ggatgaagtg ctgcctcccg aaaagtgggg
    40621 cgcgggctcc agcagcgtgg aacccgccta cagcgggctt caacgcgatt tccctgccac
    40681 caatgaaacg gatgcaaatg ccaccacacc cgaaaaagcg gagcttggcc gcctgctttt
    40741 cttcgacccg atcctttcaa aaaatgacga catgtcctgt gccacctgcc acaaccccga
    40801 tctcggtttc ggtgatggca tgcagcaggc actcggcgcg aacggcaaga cccttccgcg
    40861 caatgcaatg agtttgtgga atgtggccta caacaccaat ttcttttggg atggaagagc
    40921 cgccaccctc gaagagcaga tgcatacccc gatcacaaac gcggacgaga tggctggttc
    40981 gcccgaagaa atagaatctc aactgaaagc gattcctgcc tacgtttcgc tattcgagca
    41041 ggctttcgga agcaaagatg ccgttacgta cgagaatgtg caaaatgcca tcgccgcgtt
    41101 cgagcgcaca ctcattacaa acaactcccc ctttgaccgt tatgccgcgg gcgatgtgaa
    41161 tgccctcacc cccgcccaac gccgcggact ggcgaccttc cgctcggcag gcacgcgctg
    41221 ttttgaatgt cacgccgcac ccaccttcag cgacgactct ttctcggtaa caggcgtgcc
    41281 cgacctgccc ggacaagagc atgatgcagg ccgcgccgaa gtggaaagtg aaagtttgga
    41341 cggcgccttc aaagcgcctt cgttgcgcaa cattgccctc tcggcgccat acatgcacaa
    41401 cggcgccttt gccacgctcg aagaagtcgt ggagttttat gccaaaggcg gcggtcgcga
    41461 tgacggcgtc gagaatgtgg atattcacgt tttgggcttt gacctcacca agcaggaaaa
    41521 atccgacctc gttgcgttca tgtacgcgct caccgatgaa acgaacctgc ccgaattccc
    41581 cgaatctgta ccttcgggtc tgccagtggt ggcgcatgtg gaaaaccccg cgcgcgaaat
    41641 tgtcagccag atgaatgccg ggggaacaga atcagtttca acgtctacgc atgaagcggt
    41701 gactttgaga gtcgaaaaag gtcaaagcat ccaatctgtt gtagatcagg cgctgcccgg
    41761 tgacaccatc gaagtgccgt acggaattta caaagagcat gtcattattg atatcagcga
    41821 tatcaaattc ttcggcattc caaatgaaga aggccagtgg cctgtgatcg aaggcgaagg
    41881 caagggtgca gatggggtga tcgcctcggg gaataatttt gaaatggcgt tctttgcggt
    41941 caagaatttc acaagcaatg gtgtgctggt ggaaggcgcg aagaatgtgt acctgcacga
    42001 catgtatatt gaaaatacag gcgtgtacgg cgtgtacccc gtgcgctgca ccgatgtgct
    42061 gatcgaacgc attgaaggca cgctgatgaa cgatgccgcg atctatgcgg gcaaatcaga
    42121 aaatgtgatc atccgcgata cgctgaccta cggaaacgtg atcggtgtgg agttggagaa
    42181 tacggtcaat ggtgaggtgt acaacaatgt agcccacgat aacaccatcg gcattttcat
    42241 tgacctgctg ccccaactgc cctccaaggt ttcgatgtac accaaggtgc atgacaatgt
    42301 ggttgaaaac aacaacggcg caaactttgg caagcctggc acagcggttt cgctcatccc
    42361 gcctgggacg ggcatgttga ttctcgcggc ggaccatgtg gaggtgtaca acaacaccat
    42421 ccgcggaaat aagacgggcg gcatggcggt tttcaatttg accatcggtt tttcggaaga
    42481 ggagatcgat gtcggtccga accccgagca taactacatc cacgataata tctacgaaaa
    42541 caacggctac gatgcagata agttcgtgaa agacatgctg ggacagggtt tcgatatcat
    42601 ttgggatggc acaggcgcag acaatcgctt cgacgagccg aacgcttcat cctcctttcc
    42661 gcctgttttg ccttcgaaga atttgccagc gccgttgtat aacctgtact ggcgggcgtt
    42721 aaattttgtg gtggggttgg tggggtaaag taataagtaa gaggaaagaa gaaagaggca
    42781 gtggcgcgag ttactgtctc tttttgtttg tgggtggtat ccccaaaagc catgacattc
    42841 gccacttcac cgcgaggcgt tttttcatat aatccagatt gcgtggggga aaagcaagaa
    42901 cttcccacga tcaaaatacg acaatgaagg aatttgccat gaaaaatgca ctgcttgtat
    42961 tgtttttggt cagcgtattg acggcttgta cgccagcaat tacctctgca ccaactgaaa
    43021 ccatccttcc aatgatcgtt ttatcatcaa cgccaattcc aacatcaacc ttaattcctg
    43081 ccactgaaac tccatcccca gtgccaactg accccatcat tctcatgatt accccagatt
    43141 caattcaagt ggagcggtgg agagagtatc aaactgctct ggcaaaaact attatgtctt
    43201 atgaacttgc tttatgcgaa tgggaggttt taggacgttc tgaaaacgaa atatatgtat
    43261 gggcaacgtg tcgaatttta gataatggat cagactctcc cgcagtgatt caccttggcg
    43321 tagacggggc tatacaaaag gtggaaattc ctggaacgaa ttggagttct gatattcgtg
    43381 aaatgttccc catagagata caagaaattt ttcttaataa cttaatgaat tatcagcagt
    43441 tatcagatca ccttacatgg agaatagagc acccagaaga atctccattg atcgttctca
    43501 gttcaacccc cgctccatag cccgattact caggtcgttg tcttcaaccc aaactacaac
    43561 aaactaatcg gatcaacctc aaccctgcaa tcgctcaatt ttagatcgcg cagcagtgaa
    43621 acggggtctg gcccgcgcag gatgatctgc cagcggtgga ttccatccac tttggagaag
    43681 aaggcgggga caggcccgat caattcagtt tggcggcggt tttcggtttc aatgcggagt
    43741 ttgagtttat cggcggcttt ttgtgaatca gattcggctt tgagcgggtc ggcatcgcgg
    43801 aattccaacc gcaccagttt tgcaaaaggc ggatacccca actgcttgcg atagtccaac
    43861 tcgcgttcgt agaatccgtt cacgtcatgt cccgccgcaa attggatggc gtaatgctcg
    43921 ggcatgaagg tttgcaaaac caccttccca cccagcgacg aacgccccgc gcggccagca
    43981 acctgagtca gcgtttgaaa gaccttttca ctggcaaacg ggtcaggcag gttcagcccc
    44041 acatctgcca gcacgattcc cacgagagtc accaacggca ggtcgagtcc cttcgagagc
    44101 atttgcgtcc ccaccagcac atcggcctga tgattggaaa aatgtgtgag gatcatttca
    44161 tgtgcgtcct tgtgacgggt cgtgtcccag tcccaacgca gggtgcggac tccggggaac
    44221 atggcctgca cttcctcttc cactttttca ctgccaagcc catactcgcg gatctgtttt
    44281 ccattgcaat tcgggcaggt cttcggcttt tggcgggtgt aaccacaatg atgacaaagt
    44341 aacgttggaa cgtttgaacg ttcaaacgtt ccaacgtgca acgtcagcgg cgtatcgcag
    44401 ttggggcatt tgagcacgaa cccgcaatcg cggcagaaaa tgtaagtggc ggtaccgcgg
    44461 cggttcaaaa acaaaatcgc ctgctcgccg cgtgagatcg tttcagcgag tgattcggcc
    44521 agcgcgcgcg aaaagattcc gcgattgcct gtcttgagtt cctcgcgcat gtccaccact
    44581 tgcacgggcg gcaatgcaga atcggtgacg cgattcggca gagtcaattt tgtcagcgtg
    44641 ccgttttcgg cggcgaatct ttgctccacg gtgggcgtgg cggagcccag cacacacacc
    44701 gcaccgcaca aacgcgcata ttcctgcgcg gcaactgcgg cgttatagaa tggcggctca
    44761 gtctggtgat aggaactgtc gtggcattcg tccactacga tcaagccgat atttggcagc
    44821 ggggcaaaca aagctgaacg cgctccaatg atgaccttca acttgcccga acgcgcgcgc
    44881 cgccacgtat cgtagcgctc cccttctgaa agtttcgaat ggatcaagcc cacctgcccg
    44941 gggaagcgcg ctaaaaatct gcgcaccgct tgcggagtga gcgcaatttc aggcacgagg
    45001 atgatggcct gctttccgcg cttgatggtt tcttcggcgg cacgaagata gatttcagtt
    45061 ttgccagaac ccgtcactcc atgaagaaga aagggtgaag gctgaaggat gaaggatgaa
    45121 tgcgttattt tttcgaggga tgaactttgc tctgaggtca gttcaaattg tctgccgtgt
    45181 tcttcttttc cattttcaat tctctgcaga gaatcgcgga agatttcatt ttcaaacagg
    45241 cgaatcaatc cgcgctcttc gagttcctgc aagtcggcga ggttgcatcc tgtttcggca
    45301 tagacccacg aaaggttgat cgcttcaggc tgctggatga gataccgcaa tgcagattga
    45361 cgacgggcga gagtctgctt tgtgccaagg gaatccatct ctgcttcagc cgcttctggc
    45421 gtaaccgaca actgggccac gcgcacgaac tttgatctca cccgaggcgg aggcagcacg
    45481 tgcttcgttg cgagaatccc ttttctcacg agggcttgcg ccgttttgcg ccaatccacc
    45541 cgcgagaaat gagaatcgat ctgacgtccg cgcaggggac cgcgctcacg caaaagtgtc
    45601 agcaaacgag tcgcgacggt tgaggtcttg tcttcgtaat tggcaattcg taattcgtaa
    45661 tggacgtccg cttgctgact caggcccacg ggcagcatga ggctgatgat ggcggccagt
    45721 ggattcaggg ttgattctgc caattggatc gcaagagcaa gctgcggttg agtcagaacg
    45781 ggggcagggt cgagcagttc catgatgggt ttgggattct ccaccgcagg agtgtcgatc
    45841 aaagcggtga cgatcccctg caccgtctgc tttccaaatg ggacgatcac aaaactacct
    45901 gcctgaattt ggagggcaag ttcgttgggg atggcgtaat caaagaggcc tgatattaca
    45961 gaaacgttaa cggcgacacg tgcaaacatg gtgagattat aatgttgatg aggtcgaaaa
    46021 ggaggcagac atgttgacaa tcatcatgat cctgggcggg attgcaattt tggcatcggt
    46081 aatactgctg gcgtttatgt tcataaaatc gaacgaagta aacctgacgg ggaaaacgga
    46141 agacaagcct gagtggatga aatcgaaccc accaaaagaa actgtgaaag ccacacgcgc
    46201 cgaaggtgaa ggggtgacct tgttcgatca cgatgcgggt gaaaagatcg cttctccctt
    46261 tgccgaacag atcgaggata ttctgcgcgc caaattggaa agtgaccctt tcaacaagtt
    46321 tgatattgat tttggttctg cgcccgatgg ttcactggaa atttgggtga acggcagcat
    46381 gtatcaaagc gtggatggcc tgcccgatga aggcttgaag aatgcttttc gaaatgcagt
    46441 aaaagagtgg gaacggttga gatgattcag tgaacgggaa aaagtgatca gaactcagtg
    46501 ggaagttgac gggcgcaaca caactcctgc gccatacccc acgaccgatg aagtatcccc
    46561 agtgacatca cccgaggtgg catatttcag gatctgcact ttgtctgcgc cgagttcgcg
    46621 agcggcatac aacatggagg caactgcggc atgtccgcag gcgaacccca ctcccgtttg
    46681 ttcggcatca aaaacagact cagggtcgaa agactcgaaa cgcctgagca tttccgcatc
    46741 gagaatgcct gcggttttct ggttgtaaaa atgggaaagg tctgtggagg ctacgatgat
    46801 ggcgtttttc cctttgagcg tttgggcgag ggcgtgaccg agtttttgtg caatggtcac
    46861 atcctgcgca cggatcatga tcggcagcaa tttgaattcg tttttgagca cccgctgtaa
    46921 aaatggaagt tcgatttcga gcgaatgttc ccgatcattt gcaatggctg tgatgggaat
    46981 ctcgaggtgg gtctgcaact ctgcaagcgc agcctgatcg actgacaggt ttccaagtgg
    47041 agtgaaataa gcctgatgct ttgtggtgat gagtggaaaa tgtgcgtagt tgtgatatgg
    47101 agaaagcaca gcgaccaatt caggggcaag tccatgcagg gtggcaaagg catgcgcggc
    47161 aactgctcca gagtagaggt gccctgcatg tggtgcgatg agtgccagca cttccccatt
    47221 taattggggc acactcgcat gagccagaaa atcatccacg ctgcgggcaa gggcggtggg
    47281 attcgcgtca taccattggc cagcaatcgg agagggtcga aggtcaagcg tggtcatcag
    47341 attcattgta gcacaaggag tgggtcaaaa ttcgtgccgt accaggtcaa ttatgcaaag
    47401 gaagttctta ttttctacaa catg
//
LOCUS       DAOQJC010000010        47010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_319_length_47010_cov_10.459972, whole genome shotgun sequence.
ACCESSION   DAOQJC010000010 DAOQJC010000000
VERSION     DAOQJC010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 47010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47010
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_319_length_47010_cov_10.459972"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PLE39_02555"
     CDS             <1..516
                     /locus_tag="PLE39_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNE67231.1"
                     /translation="QVGATIVHRVLTSLFVDRGVKIDRTYQLNFGGNTDFLNMLERER
                     LESKKISKTNAVTSMIPYEIDPSNVHVGPSDHVPWLTDRKWCYIRMEGTTFGNVPLNM
                     ELKLEVWDSPNSAGVMIDAVRCAKIALDRELAGPIVGPSSYFFKTPPKQFKDEIAHQK
                     VEDFIAGRSDE"
     gene            572..1726
                     /locus_tag="PLE39_02560"
     CDS             572..1726
                     /locus_tag="PLE39_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNE67232.1"
                     /translation="MIKKRLFPTLILLAILLAGCQPAAPTADSVAIQTAAFETALAQI
                     SIPTQTPAPTDTAIPTATVPRTPPALPSIYQSPILKPEDLPRTYVADTCTYLQSKWNP
                     NNAAPGTVVMPIMIHAIDQDANSSQLLKISGKDFRQMMNDLHEFGFQAINMQQMADFM
                     YNNARIPQRSVLLIVDDRAYAEKFNTHFRGYFEDWGWPVVNAYIGLDERPDLWAENAA
                     LSAEGWVDYQSHGYIHNIPITDSSTDEYITAELSGAITSIQKYMNKTPIAYIWPGGGF
                     SRRAVELAPQLGYQLGFTVNPRGPVMYNWVPQADAFNASNPYAIPEVPAGNPLMTLPR
                     YADINARSELDNVRQISEQAAAYAEQNKATELEYYDIMCAPTYGPIPPAQ"
     gene            1953..3056
                     /locus_tag="PLE39_02565"
     CDS             1953..3056
                     /locus_tag="PLE39_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67233.1"
                     /translation="MKPFPESLRTILYRLTTLLIIVFILISCEPFTTSQQIEAQSISS
                     TDTALAQANHTQAPPRTPPALPAVYQSPYLNPLDTPRTYIQNPCQYLHNKWNPLSADP
                     GTVVMIVMFNDILMEDFDRIMEQLHEQGFEAINTKELLAFMERNVKIPQRSVVIIQND
                     SQQADDLTRNYREYYDRWKWVVVNGWTSNPDAPEYLWQDNIALENEGWVDHQAQGMIP
                     GTVLSDESSKTVIQRELENPITAFIKLYNKNPIAIIWPGGGFGMRPVEAARQLGYQLG
                     FTANTRGPVMYNWVPLADEVDPERPTYIPEGQINDPLMTLPRYWPEQIMDAIDEVRLT
                     GNEASAYAKANKKAELEYYDIVCADTYGPMPTP"
     gene            3081..3425
                     /locus_tag="PLE39_02570"
     CDS             3081..3425
                     /locus_tag="PLE39_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNE67234.1"
                     /translation="MSDCIFCKIIKGEIPSTGVYRDEQVTAFRDINPAAPTHVLIVPN
                     KHIDSVNMMSAEDEPLLGHLFMAAKTIAAQEGVAEGGYRLVVNTNANAGQTVFHIHLH
                     LIGGAPLKLQMG"
     gene            3610..5031
                     /locus_tag="PLE39_02575"
     CDS             3610..5031
                     /locus_tag="PLE39_02575"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNE67235.1"
                     /translation="MPEQEIYLLSPRALSAETIAVAFAKTSRAPESFREIAAELSDEK
                     SAQFHEKWVVGYGHASVAEHAVLHIAFENVSRVAIETIESSRLASYTEKSTRYQKWGP
                     DDFTIPPELQEVGHPLRDEFIQTVRLLFSTYAESLEPVKSLVVERTPRRDNESDEAYD
                     RRIRSQYVDRCRFILPAAANANVGMTANARIIEMVIRKMLSHPLAEVRQIGEKVKEVS
                     KAETPTLVKYADAVQYVVETVNQLSANSEQLSVKSGRADWCALIDYDKDGEKKILAAA
                     LYRFNEMPYKEALAYVKTLKKKEREGLAEALLGKLGKYDVPLRELEYATYTFDLVMDQ
                     GAYAEFKRHRMMTQTPQRLTTRLGFTIPLLVTEAGFGSRYEAAMQSASDMYEKLHAFN
                     PDVAQYIVPNGFNRRVLAQFNLREAFAFCQLRSAANAHFSIRRVAQRIYEEMTRVHPL
                     LTKYMKLREETWQQVEENYFTQV"
     gene            complement(5103..6086)
                     /locus_tag="PLE39_02580"
     CDS             complement(5103..6086)
                     /locus_tag="PLE39_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE67236.1"
                     /translation="MKYRHLGKTGIQVSELSFGSWVTFSNQADVKAAVDMMAAAYDAG
                     VNFFDNAEVYASGKSEEVMGAALKQLNWRRGSYLVSTKLFWGLHDGINEKNTLNRKRL
                     IEGIRGSLQRLQLEYVDLIYCHRPDKTTPIEETVWAMHNIIEWGHALYWGTSEWAASE
                     IVEAIQIAERHHLHKPVVEQPQYNMFVRNRLEGDYERFYKEYNYGTTIWSPLASGLLT
                     GKYKGGIPKDSRGALKGYDWLQNHLTDKQKLAKVNALEPIAKELGCSLSQLALAWCAK
                     NPFVSTVITGASRVDQVHENMKAMEVIEKITPEIMARIDEIFEIKKDEDDD"
     gene            6159..7067
                     /locus_tag="PLE39_02585"
     CDS             6159..7067
                     /locus_tag="PLE39_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE67237.1"
                     /translation="MQKTLILCAMLLSACASSNGQPSDGPLQPYLTRTPVNTSTPNTI
                     VIINTPIPTATPFTYIIQQGDTFSELAEKFKVTQDDLQAANPDVSPNSMSVGTTLLIP
                     GSFLTAADASTPTPVPAPVTQSICHPAADGGLWCFAMIQNNSAGFLENVSAQITLDPN
                     ITATAFTPLDIIPPNSSMPVYVFFPNTPAALGANPQVQVLSAIQLSANPPRYLKATLN
                     NAIAQIDWDGRSAQLSGEILLPAESTAATQVWVAAVAYDQLGQVVGISRWEGEAIQAG
                     GSLKFNFAVSSLRSTIDAVDFFIEAR"
     gene            complement(7126..8751)
                     /locus_tag="PLE39_02590"
     CDS             complement(7126..8751)
                     /locus_tag="PLE39_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE67238.1"
                     /translation="MANPFSLKFRRGSFAIRSLLLVLVCSLILSASARDVFAFQVDIP
                     FDQALVYSGGESTNLRDYDPATTYSSGDKLVFSGLVSFDPHLNLTPDLAETWDVSDDG
                     TVYTFHIRANAKFHSGRFVTAQDFIYSWERAVSPELASDTALTYLGDIVGVREMNTGQ
                     AEHISGLAAIDERTLQVTIDAPKPYFLLKLTYPTAFVVDQANVESGEEWVRDPNGTGP
                     FRLAEWVSNEYKVYAANEDFYLGMPSIPYVIVKLYAGDDVRLFETGDVDVAGVGLYSV
                     DRMLDPEEPLHNQLVTGVNLCTGYVVFDTTQPPFDDVNVRKAFSMAFDRQRYIDVVLR
                     GRALPAFGPFPPGLPGFNYQLKGLPYDPQQARELLKQSKYGGPEGLPPIVYTNGGIGS
                     YISSSVAATVEMWEQNLGVNLTVENIEYNFYNQQIYSGNHGQIFGGGWCADYPDPENF
                     ADVLFHSGSKQNNSGYSNPALDALLEQARIEQDVTKRIAMYQQAEQMIVDDAPVLFTT
                     HSLSYELVQPYVKGYVFTPIAVPLERYLWIDGK"
     gene            complement(8744..10108)
                     /locus_tag="PLE39_02595"
     CDS             complement(8744..10108)
                     /locus_tag="PLE39_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HNE67239.1"
                     /translation="MHLRKILSVLLLLAVVPVQSVFAAAGAEVQDDAVVFDFPNTATF
                     SATLKSAAAITAVTLEYGNEQQTCGEVIAKAFPQFDPSTTVQVEWTWDMRQSGSLPPG
                     STIWWRWRYTDESGAEFVSDQKTAIWLDDVHNWQTLSSDELRVHWYDNDKAFAQTMLD
                     AGTEGLRRNREQAGLSIEDVVDIYVYPNYDDMRDAILYEPSWTGGMAFSDHNIVIMGI
                     SASDSNWDKNTVIHELTHVLVGHFTFSCIGTVPTWLNEGLAMFSEGELDSYMQSQLDD
                     AIKNDTLLTIRSLNGGFSELPDKANLSYSQSHSIVEFLISTYGQDKMTEILAALRDAK
                     PIDDALIEVYGFDTDGLEDAWREAIGAAPRTVSAQPTAQPTPTFVPTYVPVSGAPLAV
                     TPTPYSVPTSSFVNTTPPIAKGPPIALTIALICMCGVILLILGVMVIGILARSQNQKA
                     GKNG"
     gene            10407..10889
                     /locus_tag="PLE39_02600"
     CDS             10407..10889
                     /locus_tag="PLE39_02600"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNE67240.1"
                     /translation="MQKNKFILGGLLILAAVVYLIFSSTQASAEYFMTVDEIHAEGDT
                     AIGKSLRLSGAVLGDTIQYDADTLTLTFEVAHVSGDNAEIEAQGGLAVVLKDAVNDPS
                     RQRITVVYVGPKPDLLRGEAQAIMTGTLGDDGIFYADELLLKCPTKYEEAVPEQAATN
                     "
     gene            10973..13039
                     /locus_tag="PLE39_02605"
     CDS             10973..13039
                     /locus_tag="PLE39_02605"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HNE67241.1"
                     /translation="MFVEFGYGVLLVSFLVAIYSIFAAVYGVSNKSTAMVESARRAML
                     LTFPLLSISAAVLVYLLVNNHFEVSFVYEVTSRSMPTYLKVTAWWGGQAGSLLFWSWL
                     LSAFASAVTIRKWERDIEFLPWVIVICSTTLVFFVGMVAFYENPFTRFWLVGGNVEPH
                     LFAPLNATLFTADDGNGLNPLLRHPGMVIHPPMLYLGFVSFVIPYAFAMAALITGRTD
                     DRWIRLTRRWSLWAWLFLSFGLVLGGRWAYDVLGWGGYWGWDPVEIAAFMPWLTGTAF
                     LHSVMIQEKRGLLKHWNMILIILTYALVVFGTFLTRSGVLSSVHAFANSPIGPLFMGF
                     VSISLIVSIALVIWRWPELRSETEMKSMLSREALFLLNNLLFMSVLVVCFWGVIFPLI
                     SELFTGQKVTVGPPFYERATAPLFAALMFLMGVAPLSAWGHSTVTTLGKAMWKSAVAA
                     VVVTGILFATYTQNVIALVGFFLIAFVIFVTVQEFWRAARARQRAQNENFFTSLTRLM
                     GRNRRRYGGYIIHISMMLMAIGILGIEIFQTETQGSLAKDEQLSISGYTLQYRELASW
                     DDRGEGVNYTRAVVDVYKDGEYIGELNPRIDYYFDSQQNMTIPGNRSTLKDDLYVLLV
                     DWQPVSNTGATFKIFVNPLVNWLWIGSLVFMLGVIFAAWPDRDPAEEPVRSGAARTAR
                     QTSAAD"
     gene            13111..13605
                     /locus_tag="PLE39_02610"
     CDS             13111..13605
                     /locus_tag="PLE39_02610"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNE67242.1"
                     /translation="MKKLFYSFTFAVLMAALFTGISFAQDTTPPTDDEVNAVARQLYC
                     PVCESTPLDVCPTEACKDWREQIRVMLSEGKSEEEILKYFEAQYGARVLAEPPKKGFY
                     WLIYLLPPAIILLGAVILLRSMKQWTTKPVAESTGMDEAGDASSAVKDDYVARLEEEL
                     KKQK"
     gene            13654..14223
                     /locus_tag="PLE39_02615"
     CDS             13654..14223
                     /locus_tag="PLE39_02615"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNE67243.1"
                     /translation="MTEETTNTKRGVPVWVQVVIWAFLVGLLGLVGFGLNRARNPMAE
                     IGKPVPDFDIVYYEGYEYDSQPQMKLSDLQGKIVLINIWASWCKPCEQEAPELEEAWQ
                     FYKNSNDVVFIGVDYVDTPAGANEYLTKFNITFPNAPDLQSTISSILNRQMGVPETYI
                     IDREGVLRHIKIGPFASVDEIKSIIQGIE"
     gene            14236..14748
                     /locus_tag="PLE39_02620"
     CDS             14236..14748
                     /locus_tag="PLE39_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE67244.1"
                     /translation="MELGSIFLVLAVIVVVFAYLYAPFTARARRLRNGETHQVSSLKA
                     ERDRVINSLQELDFDFNLGKIPEGEYPEQRAALLQKGADILRKLDELAPVSSSALNAE
                     ARIEKATAAGRADSAAVSAVLDSDDDIEAMIAERRKSHKSKSAGFCPKCGKPVLSADK
                     FCPSCGKALK"
     gene            14790..16652
                     /locus_tag="PLE39_02625"
     CDS             14790..16652
                     /locus_tag="PLE39_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNE67245.1"
                     /translation="MKLRHIVFITAIAILLTACNFTLAADVTPPPGYASPTPMPTLGP
                     LYPASNPDIHNGAVIYMEKCAPCHGNAGLGDGPQGKELPVTVAAIGLPATATKAKPSD
                     WYTQVTQGNLERFMPPFSSLSDQERWNVVYYAFTLHILPEQGDLGQTLFEENCADCEK
                     YFSDLKTMSALSEDEIVQIIKNGSGEIPAFGKDFSDDEALAVAAYLRTVIFAPPPAQP
                     TPVPASETPLSAEAGTPSAVETPNGTEQAQTTPEADNSFLTTFGNISGTVDNQTGAAL
                     PSNLTVKLRAFEHGGDPSAGPEEVDSTEVTINADGSYQFENMEIPESRIFVAELELEG
                     ISYQTDFAIVEAGMTSLVLPPLTVYATTEDLSTLKINEAQMYFDFANTEDVQIFTVYS
                     FTNTGNKTILVNMGADQVVPFIAFPKDAKALGYEAMQNSAAFVPTDGGFAMPPSELPY
                     GLIAFASVPKAREVAISQSAVLDIESISLYLPQGMEAEGATLSDAGLQNMETMSFQVY
                     SATGLKKGESIEFTVSGEPSATSETADLTQNQTLLIGIGAFGLVLIIAGGWLYIRDRK
                     KEEDLDDEDGEFEDTESTMDAIIALDDLHREGKISDEAYQKRRAELKSALKKQK"
     gene            16739..17437
                     /gene="ccmA"
                     /locus_tag="PLE39_02630"
     CDS             16739..17437
                     /gene="ccmA"
                     /locus_tag="PLE39_02630"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HNE67246.1"
                     /translation="MIEVKKLVKRFGLKVILRGLDFQVQPGEFVALLGPNGAGKTTFL
                     RILASLSRPSLGEVRVAGYKLPHEAAQVRARLGVVSHLPLLYPDLTAEENLRFYARMY
                     GIEDQEARITEVLGMVGLEPRRRDLVGAFSRGMQQRLAIGRAVLHDPEVMLFDEPYTG
                     LDQDASEMLDEVLRSVAAKGRTVVMTSHDLARAEELATRFDILSRGVIATSAKREDLK
                     STNLLAFYKQSLES"
     gene            17437..18123
                     /locus_tag="PLE39_02635"
     CDS             17437..18123
                     /locus_tag="PLE39_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNE67247.1"
                     /translation="MKRSSPSLLKATLAIVQKDLAAEFRSRELLSAMLVFSLLVILIF
                     NFALELDVKVRQSVTAGVLWTTFAFAGTLGLNRSMAVEKDRGCIDGLLLAPVDRSAIY
                     FGKAISNLAFMLIVEAIVLPVYSMLYNINLFQLDLLGVILLGSIGYTAVGTLLSAMAV
                     QTRTRDVLLPILLFPIAVPVLLAAVKASGGMLAGAEFAEILVPLNILIVYDIVFIAVA
                     FMVFDFVVEE"
     gene            18203..18898
                     /gene="ccsA"
                     /locus_tag="PLE39_02640"
     CDS             18203..18898
                     /gene="ccsA"
                     /locus_tag="PLE39_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNE67248.1"
                     /translation="MQPKPTALKVLDIASIIVLAISTYLALFFAPTELVMGDVQRVFY
                     FHIGTAWVGLLGFIIAAGSGIIYLITKDLKWDRFEVAAVEVSTVFFFLTIVLGSIWAR
                     PAWNTWWTWDPRLTTAAVTELIYVAYFMLRQGIDDPEKRARFGAVYTLLGGISAPVTF
                     MVIRLFRSIHPVVIGAANANQEKMSMTPDMRVAFFFALFAFTVIFIDLIWHRLRLGSM
                     EEKVEQLKLKVSM"
     gene            18913..19083
                     /locus_tag="PLE39_02645"
     CDS             18913..19083
                     /locus_tag="PLE39_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67249.1"
                     /translation="MESTPDTSSYMIAGYVISFITMGIYVASLYIRNNNILRDIETLE
                     SMQEEKPKATKK"
     gene            19135..20781
                     /locus_tag="PLE39_02650"
     CDS             19135..20781
                     /locus_tag="PLE39_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67250.1"
                     /translation="MNMTRRWMLIFLALTVACSGAVFTRDISEALGNGFQPVSANNPP
                     PGPDRYTYITVQYTAYEWYLATWKDQVPLCSLITDHEGMPLPGEVYRDCGETIYEKWI
                     VQKPCMATNKRSCTGFYISPVKNYPAEKEVPAELEPATVWVSLEGCETVLSTSTNFCE
                     VAPTLVFTGQEPLPGETIIRVEGTYDGAPFKCEGTNVCKFEIPSTDEDGAKIEFWAFS
                     SYGDSSLIYSAQIRVQKVDEGDPDQLYWYADVLSTQWTGEAVATCSGAWGVFPPVGGP
                     PTWLTTPKYSEELSSDIPYTYLAANLIRQGVVDASSCADGGLTPEGGVNQCGLEAARP
                     AVTQWQNQFDELILTTAQQTAVPARLLKNLFARESQFWPGSFQGMDDTGLGQLTENGA
                     DTTLFWNSSFFEQFCPFVLSEETCGNGYVNIKEEERAELRRALVGSVNATCENCPLRI
                     DLPQANYSVGVFAHSMIANCEQTGQVIKNYTGQNAGEVASYEDLWKFTLVNYNAGGGC
                     LAEALTYTLGSSLPLTWENVSPFLAGACSGAVDYVNDISK"
     gene            complement(21079..22305)
                     /locus_tag="PLE39_02655"
     CDS             complement(21079..22305)
                     /locus_tag="PLE39_02655"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNE67251.1"
                     /translation="MPAPRISRRQFLQGCSAAIASMAGARLTNLAFAKQDNPSDTLVV
                     VFLRGGWDALNVVPPLQGDDRGFYEKARPDIKISNLLSLNDQFGLHPALAPLHELYQA
                     GKMGVVHAVGLNHDTRSHFDAQEYIELGTPGSKNTTSGWITRHLQETGVSSILPVVST
                     TGQPTSLLNFVPTVNVSTPEDFSQWGNDLIGSQQTALRQMYQGETLLHRAGLRTLDAL
                     DIVSPLVSQEYQPANGAFYNDDEFGQQLKTIARMIKLDAGMRVAAVDFGGWDTHEYET
                     DGNGGYIGDLLGQLASGLSNFYLDLDSGYTNKLSVVVISEFGRRLVQNESYGTDHGHG
                     SVMLALGGNVNGGRVYGQWPGLNNDQLYDHADLAITTDYRQVLSELLSTRLGNSNLEA
                     VFPGFSPSDNPLGIFR"
     gene            complement(22312..24042)
                     /locus_tag="PLE39_02660"
     CDS             complement(22312..24042)
                     /locus_tag="PLE39_02660"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HNE67252.1"
                     /translation="MNSKQFSRRDFLKLGGSLATASLLAACQPGEETSTAPLSPTTSA
                     NDISIPEPAVSLPPVSLAILALGRMTFGIRPGDIEAFNALGSTDDERLRAFVQQQLNP
                     DSLDDSDFESRYSAAGFQALHKSHEQLYSDHIANNAYDANDDAYWEWYSLPAYELVDA
                     TLLRAVFSKKQLVELLADFWHNHFNIFFWQDDGVPLLVSYNRDVLRRHMLGNFRQMLE
                     AVASHPSMLYYLNQNNSSDAGPNENFARELFELHTLGAENYLGVRDPNTVEKDANGIA
                     IGYVDNDVYEAARCLTGWRIDDDLGEWEEGVGITGNFIYYKPWHDRFNKLVLGKYIPA
                     DQPDMQDGKDVLDLLAAHPGTARHIARKLCRRFIADLPPDSVVEKVAAAFTEFRDAPD
                     QLKRVMEVLLLSDEFKQSWGQKIKRPLEACISTLRSLNSDFTRVPGGVPWMLSLMGQP
                     LFERRSPDGYPDVKEAWANSMSLLYRWNFAVGVAENWLNDDDKGLTLHTDLLNQTPAE
                     IRTAESLADFWIQRILGRPLSSDADRNSVIAVMAQEYGTQELLPEDHVAYVLPAMVEV
                     ILMSPDFQWK"
     gene            complement(24065..24772)
                     /gene="lipB"
                     /locus_tag="PLE39_02665"
     CDS             complement(24065..24772)
                     /gene="lipB"
                     /locus_tag="PLE39_02665"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNE67253.1"
                     /translation="MFPFTIEDLGLIEYEKAWKLQDDYAAEIAEGKRPPTLLLLEHPH
                     VYTFGRKGHAENLLWNEEQLKQKGIATHWVDRGGDVTYHGPGQLVGYPLLSLGKLNNE
                     NKLPEADYVGYVRKLEETLITALMHFGIVSGQRSGLTGVWIQSDVHSRCPRCSPEDRK
                     KPAKIAAIGVKVDARGITRHGFALNVEPDMEYWNGIIACGLNEPVVSLADLLDPAPSM
                     QQVKAEVTRAFREVFAL"
     gene            complement(24828..26213)
                     /locus_tag="PLE39_02670"
     CDS             complement(24828..26213)
                     /locus_tag="PLE39_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE67254.1"
                     /translation="MNNTFTQLENFGHSLKAPSYLIKPQKSEEIYEAFRSAKQMGLTV
                     AARGGGRSYNDAALNGGGIVLDTSGMNQILEWNPATGQVRCEPGVTLKQLWQKVLPDG
                     WWPPVVSGTMFTTLGGCLSANIHGKNNFRMGTIGEHVLEFTAVLPTGAEVTCSPNKNG
                     DLFHSMISGLGMLGIFTSVTMQMKKMHSGLLEVHAWPSHTLKDHLASLEDGAPNYDYI
                     VGWLDTINTIGHGQIHAANYLHEGEDPEAQKTMQLSYQHLPDRLFGVMPKSMLHYFMT
                     PFANNLGWGLVNTAKYISSLRKHTFRQSHAAFHFLLDYVPLWELSYGKGGLIQYQSFL
                     PKETAHQAWTEMLALAKKRGLPSYLGVTKRHRPDKFLLTHAVDGFSLALDFKVTNRTR
                     VRLSAMLQDFDKIVLNAGGRFYFAKNSETSAETARAFYGEETVEKFRKLKKRCDPNGL
                     LESDLYRRIFS"
     gene            complement(26260..27048)
                     /locus_tag="PLE39_02675"
     CDS             complement(26260..27048)
                     /locus_tag="PLE39_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE67255.1"
                     /translation="MSNSPKPAQATPLNPRRRGIIVGASDGMGAALARKLAKEGYTLA
                     LIARRQDKLASLCAEINAAEGEARALAYAHDVSNYDEVPNLLRKITAELGGLDLLVFM
                     AGVNFPPGLNSYNFEGDRKMIEVNLIGAMAWLSPVAEMFQSAKAGQIVGISSVAGDRG
                     RVGNPGYNTSKAALTTYLEALRNRLTRHGVNVLTVKPGFVKTEMLKAAQGGTPFAISP
                     EQAAEDIYKAMRKRKQLIYTASIWRWIMLAIQHVPSVIFRRLSF"
     gene            27272..28543
                     /locus_tag="PLE39_02680"
     CDS             27272..28543
                     /locus_tag="PLE39_02680"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNE67256.1"
                     /translation="MAGITEKQKNIFHHAREIIKQDESIPEEEKEGIFKLIRVLVEVA
                     DLAPSKLPNQEESPTAFTQEIISRHALMAAVKQQIDELESLKKLSLNVTSSLDLQTVL
                     DTVVAEAMRLVKNSRAAHIFLYSHGKLEFGSSLTAEGLKNMPVAMPRKEGLTATVVAS
                     GVPIIVEDITDHPLYTNVSQNWRGSIIGLPLKYNKSIVGVMNLSRSTVGGFTRAELRL
                     IGLVADLAAVAISNASLHQQVAAQANTDSVTGLPNRRALDERIQEEIRFARRMNSEFS
                     VVMMDLDGFKAVNDTYGHVVGDELLYSLFNYLAENIRPTDFLARYGGDELTLVSREAN
                     QDMAESVTQKIIDLVRGYKPAFAEKKNIQLGITAGIAIYPTHSKTAGDLLRAADAALY
                     QAKKHHRGSYSIAKITTGPLDPITLPRKKPE"
     gene            28639..29562
                     /locus_tag="PLE39_02685"
     CDS             28639..29562
                     /locus_tag="PLE39_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNE67257.1"
                     /translation="MPRKVKLILNPMADMGRAWKTANDLRPIAQEFKGELTWSGTVYP
                     THAIELARQAAEEGYDLVIAMGGDGTAHEVMNGLMQVPAEKRPVMGVVPIGSGNDFAY
                     SMGITQKSAHALAHMLKAETIQSIDIGQMTDEHNRKEYFDNTLGIGFDAVVTIRSHKL
                     PIVKGFLMYLTAVIQTIILNHNPAHIKIETESEQWETSTLMVTLCNGPREGGGFMLSP
                     NSKNDDGKMEYVIVNKVSRGMMFRLVPEFMNGTHMRFKQIRMGEFKKCSITSDRPLYI
                     HADGEIFTSFGSNLRKVNFEILPQALKVVRG"
     gene            29616..31232
                     /locus_tag="PLE39_02690"
     CDS             29616..31232
                     /locus_tag="PLE39_02690"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HNE67258.1"
                     /translation="MPSLYQTFLKHDLGHLRIVADLWGLELESQDLDSAAEELSASLL
                     DLEAVTETIDILSPESRAALDAIVESNGKMEWAAFTRAHGEIRGMGAAKRDRERPHLK
                     PISPAEHLFYRGILARAFFESSKGSQEFAYIPDDLLEVIEMLVVEDDEAPTPAPHGEP
                     LGRPATPVEKAVELPATDHILDDATTYLAALRIGSRAAEGVSTRRRELHELLEAAHLI
                     KDDALQPEAVKNFLGASRAEALNILYKAWFVSNTFDELRLLPGILCEGEWSNQPQVTR
                     EFLTNLINDIPQNKWWSLPALLRDIKTKYPDFQRPAGDYDSWFIKRESDGQYLRGFAY
                     WDQVDGALIKYFVQTLHWLGRVNLAAPEEGKEPTAFFLLSSFAEKNEEKGKVIVSSNG
                     KITISRYFSRAVRYQIARFCEWDEEKGDEYFYRVTAGSLKHANEQGLKSEQLLALLVK
                     YTNGNVSPALVKALKRWDTSGVEARVESLLLLRVSRPEIMEEIRKSKAGKYLGEVLSP
                     TAVIVKSGAIQKVMAELAELGLLAEVTTHE"
     gene            31225..31464
                     /locus_tag="PLE39_02695"
     CDS             31225..31464
                     /locus_tag="PLE39_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67259.1"
                     /translation="MNRGRRIILIVAVILALIPVPMTCGHMFYGCATGPDMSGNYYTY
                     YEVEPLGITLIETLIMTNLKIYYWSGKEAHFIGIP"
     gene            31530..32708
                     /locus_tag="PLE39_02700"
     CDS             31530..32708
                     /locus_tag="PLE39_02700"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNE67260.1"
                     /translation="MSKTDWIQQEIDNLKSQGLYNNIRTIGSPQGAWLTVDGKKVLNF
                     CSNNYLGLANHPTLTAAAKKATDEMGVGPAAVRSIAGTMTLHVELEKRLAQFKGVEAA
                     ITFQSGFTANLATVPALVGKEDVIFSDRLNHASIIDGCRLSGAKIIAYEHNDVKSLEE
                     QIKANLSQYRRALIITDGVFSMDGDIAPLPDIYEVAKKYDILLMVDDAHGEGVLGKGG
                     RGIVDHFGLHGKVDVEVGTMSKAFGVVGGIVAGKSVIIEWLRQRGRPFLFSSAVTVPD
                     AAACLAAVDLLEDSTQLVDKLWDNAKYFKAEMKNLGFNTGVSETPITPIMLGEAPLAQ
                     QFSRELFEAGVFAMAIGFPTVPQGKARIRVMISAAHAKDDLDKGLEAFKTVGKKLGVI
                     "
     gene            32834..33457
                     /locus_tag="PLE39_02705"
     CDS             32834..33457
                     /locus_tag="PLE39_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67261.1"
                     /translation="MNVPIFPDNINLSRPFHFTTENADSKARIGGNMPEELSISLNEN
                     AQYFGTFPLYDNESPLYFSLFINCSFKEFVVDWHGRIKDDSKVVVALHEYRPRSKSTQ
                     YASRLKSQHSIEILKTREDIYQSDTGELVVREGHKFGGAPYCIQEPKLIGWEELQAQG
                     YRQILQIDFPGLEDGAVSGTWPFGDGIFNLFWKYPFEGEKYFWCVQS"
     gene            33618..33917
                     /locus_tag="PLE39_02710"
     CDS             33618..33917
                     /locus_tag="PLE39_02710"
                     /inference="COORDINATES: protein motif:HMM:NF015126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HNE67262.1"
                     /translation="MSTDPTFRMTIQDVFSIKGRGTVLTGRIESGTITVGDEIIIKGR
                     NSSKKAIVAGVEVRRKVTTSARSGDEAGIRLKDTEEDVQQGDILTGSEHDFTWKP"
     gene            34102..34854
                     /locus_tag="PLE39_02715"
     CDS             34102..34854
                     /locus_tag="PLE39_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNE67263.1"
                     /translation="MSGHSKWSTIKRKKGVADAKRGAVFTRLAREVTIAAREGGDPDT
                     NFRLRLAVDKARAENMPKDNIERAIKKGTGEDKEGTTFEELTLEGYGPHGSALMVTCV
                     TDNRNRTVPDMRHAFSKSGGNMAEPGAVAWQFDRKSYFAFPASKLSFDKAFELGIEAG
                     ADDVVDGGDTIEIYAPVESFKTIADALHKVHVQPEEASLRMIPKQEIELDVESTLKFM
                     KMVETIEDLDDVQDVFHNVKVSDEALAALEAA"
     gene            34903..35385
                     /gene="ruvC"
                     /locus_tag="PLE39_02720"
     CDS             34903..35385
                     /gene="ruvC"
                     /locus_tag="PLE39_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNE67264.1"
                     /translation="MTLALGIDPGTATTGYGLVRLMPNGELVTVSYGIISTPKDATPP
                     ARLEMLFDDLNKLLKKHKPETAAVEKLFFSKNVTTALAVGQARGVIMLCLQKAGIEPF
                     EYTPNEVKQAVAGYGSADKRQVQEMVRALLQLDSIPKPDDAADALAIAITHLNTKRYE
                     "
     gene            35467..36042
                     /gene="ruvA"
                     /locus_tag="PLE39_02725"
     CDS             35467..36042
                     /gene="ruvA"
                     /locus_tag="PLE39_02725"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNE67265.1"
                     /translation="MIATLRGEITQIEENAIVVEVGGVGMRVFVPAPLRTNAKAGEIL
                     FMFTHLVVREDALTLYGFESQADRDLFNILLGVDGVGPKVALSVLSTLTIDAIQRAIF
                     ADEADLLNSVPGVGKKTAQKMALHLKDKLKPTDALGKIAAMADYDSEVLAALTALGYS
                     VVEAQAAIQSLPKDAPKEVEERLRTALGYFQ"
     gene            36188..37036
                     /gene="proC"
                     /locus_tag="PLE39_02730"
     CDS             36188..37036
                     /gene="proC"
                     /locus_tag="PLE39_02730"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNE67266.1"
                     /translation="MLKKKIAFIGPGVMAEAMIAGLLRKKLADAKNITASGPREERGM
                     ELNKKYGIKATTDNAAAIHEADVVVLSVKPQRLTEVMKGLKGIRSDALVVSIIAGATM
                     KKISSGLKHKTIVRSMPNTPGQIGEGITVWTASKEVTDEQQEMTRAILGALGEEVFVE
                     DESYLDMATALSGSGPAYVFLFTEALIDAGVHMGFPRRIAEQLVLQTIKGSASYYQGA
                     SRHPATLRNQVTSPGGTSAEALYYLEKAGFRTAISRAVWAAYQRSLELGKEKPGHIDT
                     HDEESA"
     gene            37101..38363
                     /locus_tag="PLE39_02735"
     CDS             37101..38363
                     /locus_tag="PLE39_02735"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE67267.1"
                     /translation="MKKTITLLTSGTRGDVLPYIALGEGLLDAGYQIRIAAPLGFANL
                     VQQSKLTFAPFEGNPSDLLIEQGDSTPMTLGTNPIRSIRATQKFIGQARPLYRRMLHT
                     AAEACRGSDMLIHGLPTIWGAHIAEGLGIPAVRAVLQPLAPTREFPSALLPFRSSFLG
                     IGNWLTHWAVTQATWLSWRREINHARHTDLGLGAAHWLDPSLQADSNRPLTLNGFSTQ
                     IVPRPKDWNDKQVVTGYWRKNELTNNHVDRPDLQHFIDSSPNPIAIGFGSPGTRGYLP
                     MLEEALQHANAQAILTIPAKWHGEIKSKNIFPIEYAPHDWLYRRVKVAVHHGGAGTTS
                     ASLHAGIPTVTLPLAIDQFFWGEQIHKIGIGPRPIPQRKLNAENLSQAITQALTDHAM
                     TAKAKTVSAELNKENGIQAAVSQIRKLL"
     gene            complement(38380..39627)
                     /locus_tag="PLE39_02740"
     CDS             complement(38380..39627)
                     /locus_tag="PLE39_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE67268.1"
                     /translation="MITFHSPLSTFPYFLSVDNNLNSSLVFIFITIFVDLLGYGIMLP
                     LLPFYVQAQDGGAAIAGALSSLYAGTQLFAGPILGALSDRFGRRPVLLVCLFGTSLGY
                     FMLGLADTLPLIFLAVFIDGLTGANLTTAYAYIADVTTSENRARGMGMVGAAFGLGMM
                     AGPALGGLLSKYGLYVPAFTASAIALGNVIFGYFVLPESLPLEKREARSVSEMFNWMK
                     HFRSLFNQANIQKLLVTMFLLNLAFAGLQSNFPLYSNFRFHWTPTQNSFFYLYVGVCA
                     VFIQGFLFGKLQPRFGEKRLILFGLSLMVIGLAGMALAPQAWMLYPAVTVVALGTGTS
                     IPSLTALVSLRVSESEQGRLMGGTQTLLSLTNIFGPMLAGISFEVIAFSAPYWVGSIF
                     SLTALGVAYMSLRGNASHQTVLD"
     gene            complement(39652..40923)
                     /locus_tag="PLE39_02745"
     CDS             complement(39652..40923)
                     /locus_tag="PLE39_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE67269.1"
                     /translation="MTTKSDVQQHDVLIIGGGPVGLSCAYYLLKSGRKVTILDSKEIG
                     KGSGSGNAGHVVPSHIIPLAAPGVVTSALKWMLDPAHSPFGMKISLDPKYLMWLLRFV
                     LACSESNVQRGIPPLNSLGQLSAKNFAQMIAEEKFDCHYQEKGLLFLYKTEEAFSGGR
                     HEGDLMQKHGIPVSVYDKAKVHEVEPAALDDVIGGVHFTGDSHMNPALFLKMLSDRVR
                     DMGAEMLENTPVTGLESAQGKVRVVRTAVGDAKHEAEHVILAAGALTPSMARDLKLNL
                     PIQPARGYSLTMSATKQMPRHALILGERRVAVSPMGDLLRFTGRLEVGNGSMTPDPRW
                     IQRIENSAREYLRIDETLDIKETWAGLRPTTPDGVPIIGRSPNHSNLVLATGHAMLGL
                     SLGPGTGQVVAELVNGKETAFDLSPLRLERF"
     gene            complement(41000..42805)
                     /locus_tag="PLE39_02750"
     CDS             complement(41000..42805)
                     /locus_tag="PLE39_02750"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE67270.1"
                     /translation="MKHYRKFIILVMFALQTVSPVKAQEGLQFEGTIAFISTDGNIVL
                     ATGDGRQVRITADAGTMDPETGVEVTYDYLSFSPDGAYLAFDRDGYTGIGDGVFVYDV
                     LKGEIVRKFSFEEVGLPVLWHKDSDSFIDSEIDPSNDYQRNYYRQFITGEREGLFQLV
                     EGDNGYVINFDLGIVIYTEGNSQIPTFYDWANNQTTNLQINGLSASANGFWSPDKRLY
                     VHTGDEKVYSLINLDSGSVYKKIEIFDILPPREHTFPTGETYNLTSVEVYDLNQEGSR
                     LLLSDDLYLYELNLDTESVETVYTSRLDNPDSRLYGFWSGSGRVILIDEYLAGGSFFD
                     GVAGDQSLLVVEKGFSPTLVADDAEALFWLSGDSDQFLYKKYTQTAQQVSVELMLFDY
                     PVRESFRVGDLPTFHDISYDPYILNFIVDWTQKKIDLTENTLEPPLASPVPPNFSIEG
                     TPISGIQQNSQPSLPLPSGISASSLFYGGICCLLLAILLVIIAVFLSRSRKRKNTQSH
                     PEDQPQFVNSASGAEQVKQAIELAKAKQYHEAFEILEGVIKTEPNNVAAWFNLGRVFA
                     SMGNLKDAERCFLQARKLGHPKANEALIWLKKKSQ"
     gene            complement(42869..43822)
                     /locus_tag="PLE39_02755"
     CDS             complement(42869..43822)
                     /locus_tag="PLE39_02755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNE67271.1"
                     /translation="MRKGQNPAKFVKDVARPERITVALLNYIPFLSGFYAETLDVLKV
                     SLESMRKDAGLPFDLMVFDNGSCEEVREFLIQEKEEGRIQYLILSEKNMGKGGAWNVM
                     LAGAPGEIIAYTDSDVLFSPTWLSRSVELLEAFPNVGMVTARPYRTSPEFYSSTLEWA
                     RNSEHASLDEGQFIPWETFLEFNLSLGQTVEENEKVYAETKDWRIQYKGMTALAGASH
                     WQFTAYKSTLQKFLPFDMDKPMGQVRQLDKRMNDAGLLRLMVSDPLAMNMSNTLGYLR
                     GELGKERERKKEGLVRRVLEVGLVKKILLAVYNKIFSWYYS"
     gene            complement(43854..44777)
                     /locus_tag="PLE39_02760"
     CDS             complement(43854..44777)
                     /locus_tag="PLE39_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67272.1"
                     /translation="MPLITLFSAPKPFTNPHIAMIQRNAITSWTLLPDVEVILLGEEA
                     GLAEIAKELGVKHLPAVARNANGTPLISSMFQLARENSNGDLLCIINADMVLMPDFVE
                     NAKQTAKLEEKFVLLSQRWDYDITSLLDFSAGWESGLRESVRKQNQLHRPAGSDFFLF
                     PKSCYTDIPNFIIGRAGWDNWMIYKARRENWPVIDCTPSVMIVHQNHDYSHLPGGRSH
                     YDHPDTNENIRLAGGQANIRYTILDATHQLVDGKLTRPKMTSLRFTRRLELVLRSIFF
                     FLPEAMIENIARPKRWQKRFKKLFNVLTFKR"
     gene            complement(44836..45651)
                     /locus_tag="PLE39_02765"
     CDS             complement(44836..45651)
                     /locus_tag="PLE39_02765"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HNE67273.1"
                     /translation="MKPKVVAIIQGRMSSSRLTGKILANIEGQPMLTRVFTRTSRATT
                     LDEVIFATTTDASDDPVAEYCEFSGIPFTRGSLFDVLDRYYQAAKDAKADVVVRITAD
                     CPVIDPELIDHVVNTLLEDEYDFACNRLPPPWTRTYPIGLDVEACTFKVLKKAWKEAK
                     EPQHREHAMPYFYEGVQLTNLNRQLQTGVSPRGFNIALLHHTTDFGDYRWTVDTPEDL
                     EFMRQVYARFDGRDDFTWKEVLDLVHDNPELMKINEGVQHKTLKDVDKRALKR"
     gene            complement(45685..>47010)
                     /locus_tag="PLE39_02770"
     CDS             complement(45685..>47010)
                     /locus_tag="PLE39_02770"
                     /inference="COORDINATES: protein motif:HMM:NF027579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HNE67274.1"
                     /translation="MKPYTSLKSILAEFFPLHRTLASDDHDKTLEIIGSYMPDSSNYT
                     IETYTPLEKAWTWYVPERYAVHEAYLEIEGGERVVDFKNNPLHIVSYSLPVDKTLSFE
                     ELQPHLYFNEKRPHAIPWVFKYYDRDWGFCLPKNVFDKLPRDKKYRAVIKSEFITDPK
                     QGFKMATAVIHPEGGPSASAGEIIVQAHTCHPHQANDDGAGVVSTIELARRLAEKPLP
                     AGSMSVRFWFGPETIGTIVYLSHNEHLIQNLRGGIFMEMTGNKNKIAWHHTRQHTHLL
                     DRITHFIVSKSAHDERDFAAAPANDERVINGPGVNVPCISINRWPYDEYHTTDDNLDI
                     MHDDMLVGAAETAEQIIRMYATNYIPKRTFRGPVFLSGNGLWVDWRENWALNRAIEKI
                     MMRFEGELSVFEIAEQVGLDYWMVRDYIEKFRAKGFVEALPIPSEAQSA"
BASE COUNT        11846 a        12798 c        11627 g        10739 t
ORIGIN      
        1 caggtcgggg cgacgatcgt tcatcgtgtg ctgaccagcc tcttcgtaga tcgaggcgtg
       61 aagatcgacc gcacctatca actcaatttc ggcggcaata ccgacttcct caacatgttg
      121 gagcgcgaac gtcttgaaag caaaaaaatc tccaagacca atgcagtgac cagcatgatc
      181 ccctacgaga tcgaccccag caatgtacac gtcggcccca gtgaccatgt accctggctg
      241 accgaccgca aatggtgtta catccgcatg gaaggcacca ccttcggcaa tgtgccgctc
      301 aacatggaat tgaagctcga agtgtgggac agtcccaact cagcaggtgt catgatcgac
      361 gcggtacgct gtgccaagat cgcccttgac cgcgaactgg ctggccccat tgttggccca
      421 tcttcatact tcttcaaaac accgcccaag caattcaagg atgagatcgc ccatcaaaag
      481 gtggaagact tcattgcagg cagaagcgac gaataaataa agtagggaca cggcggcgcc
      541 gtgtccctac tttattggta gaattcggcg catgataaaa aaacgactct ttcctaccct
      601 catcctgctt gccattctac tggcaggatg ccaacccgca gcccccaccg ccgactccgt
      661 tgcgatccaa accgccgcat tcgaaaccgc attggcacag atcagcatcc ccacccaaac
      721 tcccgccccc accgacacag ccatccctac tgccactgtt ccgcgtacac cacctgccct
      781 gccctccatt tatcaatcac ccatccttaa accagaagac ctcccgcgca cctacgtggc
      841 agacacctgc acctacctgc aaagcaaatg gaatccgaac aatgccgcgc ccggcacagt
      901 ggttatgccg atcatgatcc acgccattga tcaggatgca aactcctcac agctgttgaa
      961 aatatcagga aaagatttca ggcagatgat gaacgacctg catgaatttg ggtttcaagc
     1021 catcaacatg cagcaaatgg ccgatttcat gtacaacaat gccaggatcc cccagcgctc
     1081 cgtcctgctg atcgtggatg accgtgccta cgccgaaaaa ttcaacactc acttccgcgg
     1141 atatttcgaa gactggggct ggcccgtcgt caatgcctac atcggtctcg atgaacgtcc
     1201 tgacctgtgg gctgaaaatg cggcgctctc tgccgaaggc tgggtggatt accaatcgca
     1261 tggctacatt cacaatatcc ccatcaccga ttcctccacc gacgaataca tcaccgccga
     1321 attaagcggg gcgatcacct ccattcaaaa atacatgaac aaaacaccca tcgcctatat
     1381 ctggccgggt ggcggcttct cgcgccgcgc cgtggaattg gctccgcaat tgggctatca
     1441 attgggattc accgtcaacc cgcgtggacc ggtcatgtat aactgggtgc cgcaggcgga
     1501 tgcttttaat gcttccaatc cctacgccat tcctgaagta cctgctggca acccgctgat
     1561 gactttgccg cgttacgcag atatcaacgc ccgcagcgag ttggacaacg tccgccagat
     1621 cagcgagcag gctgctgcct acgcagaaca aaacaaggcc accgaactcg aatactacga
     1681 catcatgtgc gcgccgacct acgggcccat tccgcctgca caataaaacg aaccgtgatg
     1741 ttaatgtacg ctgactctgc cgatcgctaa ccgaatcagt ttccccgtcg gtacgatacg
     1801 ccctgtccga atttcgtttt tggctcaagc cgccgtcctc ggcggcgttc tgcgaataaa
     1861 cccttcgccg gggacggcga agggagcaac tcaaccagtt tccccgtcgg tacgatacgc
     1921 cctgtccgaa tttcgtttac aataaagaag acatgaaacc atttcctgag tctctcagaa
     1981 ccatacttta ccgcctaacc accctcctca tcatcgtttt tattctgatt tcctgcgagc
     2041 ctttcaccac ttcccagcag atcgaagccc aaagcatcag ctccactgat acagcccttg
     2101 cgcaagcaaa tcacacccag gcaccacccc gtacaccgcc cgcgctgccc gcggtgtatc
     2161 aatctccata cttaaatccg ctggatacgc cgcgcacata cattcaaaat ccatgccagt
     2221 atctgcacaa caagtggaat ccgctcagcg cagaccccgg tacggtggtt atgatcgtga
     2281 tgttcaacga tatcctgatg gaagatttcg acaggatcat ggaacaactt cacgagcagg
     2341 gatttgaggc catcaacaca aaagaattgc tggcattcat ggaacgaaac gtcaaaatcc
     2401 cccagcgttc ggtggtcatc attcaaaatg acagccagca ggcagacgac ctgacccgca
     2461 actaccgcga atattatgac cgttggaaat gggtggtggt caacggctgg accagcaacc
     2521 cagacgcacc cgaatattta tggcaggata acatcgcact ggaaaatgaa ggctgggtgg
     2581 accatcaggc tcaaggcatg attccaggca cagtgctttc agatgaatct tccaagacag
     2641 tgatccagcg cgaacttgaa aacccgatca ctgcgtttat caagttgtac aacaaaaatc
     2701 ccatcgcgat catctggcct ggcggcggat tcggcatgcg ccccgtagaa gcggcacgcc
     2761 agcttgggta tcaacttggc ttcaccgcca acacccgcgg acctgtgatg tataactggg
     2821 ttcccctggc cgatgaagtt gaccctgaac gccccaccta cattcctgaa ggccagatca
     2881 acgatccatt gatgaccctg ccgcgctact ggcccgaaca gatcatggat gccattgacg
     2941 aagtccgcct gactggcaac gaagccagcg cgtacgcaaa agccaacaaa aaagcggaac
     3001 tcgaatacta cgatatcgtt tgtgccgata cctacggccc aatgccaacc ccgtaaccaa
     3061 ccatctttcg gaggtctccc atgtctgact gtattttttg taagatcatc aaaggcgaaa
     3121 ttccaagcac gggtgtgtac cgcgacgagc aagtgactgc cttccgcgat atcaaccctg
     3181 cggcgccaac tcatgtgttg atcgtgccca acaaacacat tgactcagtc aacatgatga
     3241 gtgccgaaga cgagcccttg ctgggtcacc tgtttatggc agccaaaacc atcgccgcac
     3301 aagaaggcgt tgccgaaggc gggtatcgtc tggtggtcaa cacgaacgcc aacgcaggtc
     3361 agaccgtttt tcacatccac ctgcacttaa tcggtggagc gcctctgaaa ctccaaatgg
     3421 gatagggttc tgtttaagat aaaataccca gcgatcagca aagcctgctg gtcgcttttt
     3481 tataacccag gccattgacc gaggacttaa attcgtccat ttgacatctt agataagttg
     3541 caaggaatgt ctttgaaatg cctttttgaa ccacgaagac actgaatcac ggagttttta
     3601 ggagacccaa tgcccgaaca agaaatttat ctgctttccc cgcgcgcgct cagcgcagag
     3661 accatcgccg tggcgtttgc aaaaacctca cgcgcgcccg aatcctttcg tgagatcgcc
     3721 gcagaactaa gcgacgaaaa atcagcacag ttccatgaga aatgggtggt cgggtatggt
     3781 catgcctcgg tcgccgaaca tgcggtcttg cacatcgcat ttgaaaatgt ttcgcgtgtg
     3841 gccatcgaaa ccatcgaaag cagccgcctc gcctcataca ccgaaaaatc cacgcgctac
     3901 cagaaatggg gacctgatga cttcaccatt ccgcctgaac tgcaggaagt ggggcaccca
     3961 ttgcgcgacg aattcatcca aaccgtgcgc ctccttttct cgacctatgc tgagtcattg
     4021 gagcccgtga agagtctcgt agtggaaagg accccgcgcc gcgacaatga aagcgacgaa
     4081 gcctacgacc gccgcatccg ctcgcaatat gtagatcgct gccgattcat tctgccagca
     4141 gccgccaacg ccaacgtagg catgaccgcc aacgcccgca tcatagaaat ggtcatccgc
     4201 aagatgctct cacatccgct tgccgaagtg cgccagatcg gcgaaaaggt aaaagaggtg
     4261 tcaaaagccg aaacacccac cctggtgaaa tatgccgacg cggttcagta tgtggtggag
     4321 acggtaaacc agttatcagc gaatagtgaa cagttatcag tgaaaagtgg tcgcgccgat
     4381 tggtgcgctt tgatcgacta cgacaaggac ggcgaaaaga aaattctcgc agcggcactg
     4441 tatcgcttca atgaaatgcc ctataaagaa gccctggctt acgtcaaaac attgaagaaa
     4501 aaagaacgtg agggcctcgc cgaagcattg cttggaaaac tcggaaaata tgacgtcccc
     4561 ctgcgcgaac tggaatatgc cacctacacc tttgatctgg tcatggacca gggcgcgtac
     4621 gccgagttca aacgccatcg catgatgacg caaaccccac aacggctgac aacccgactc
     4681 gggtttacga ttccgctgct cgtaacagaa gcaggtttcg ggtccaggta tgaggcggca
     4741 atgcaatctg caagcgacat gtatgagaag ctgcatgcgt tcaatccaga tgtcgcacag
     4801 tacatcgtcc ccaatggatt caaccgccgc gtgctggcac aattcaacct gcgtgaagcg
     4861 tttgcgtttt gtcaattgcg cagtgcggcg aatgcgcatt tctcgatccg ccgtgtggca
     4921 caaaggattt acgaggaaat gacgcgtgtc cacccgctgt tgacgaagta tatgaagctg
     4981 cgcgaagaga cctggcagca ggttgaagaa aattacttca cacaggtata aacgcaggga
     5041 tacagcaatg ctgtgcccct gaaaaatcaa aaggcggagc atgagatgct ccgcctttgt
     5101 ttttaatcgt cgtcttcgtc tttcttgatc tcaaagatct catcgatcct tgccatgatc
     5161 tcgggggtga tcttttcgat cacttccatc gccttcatgt tctcgtgaac ctgatccaca
     5221 cggcttgccc ctgtaatgac cgtactcacg aacggattct tcgcgcacca tgccagcgca
     5281 agctgagaaa gtgaacagcc aagctctttc gcaatcggct ccagggcatt cacttttgcg
     5341 agcttttgct tatcggtcaa atggttttga agccaatcat agcccttgag cgcaccacgg
     5401 ctgtctttgg gaatgccgcc tttgtatttc cccgtcaaca acccggaagc cagcggactc
     5461 caaatggtgg tgccatagtt gtattccttg taaaaacgct cgtagtcccc ttcaaggcgg
     5521 ttacgcacaa acatgttgta ctgtggctgc tccaccacag gcttatgcaa gtgatgacgt
     5581 tccgcgatct ggatcgcttc cacgatctca gaggcagccc attctgaagt accccaatac
     5641 agggcgtggc cccactcaat aatattgtgc atggcccaaa cggtttcttc gatgggtgtg
     5701 gtcttgtcgg ggcggtgaca gtagatcagg tctacatatt cgagttgtaa tctttgcagt
     5761 gaaccgcgaa tcccttcaat gaggcgctta cggttgagcg tattcttttc gtttatgcca
     5821 tcatgcaggc cccagaaaag ttttgtagaa acaaggtaac tgcccctgcg ccagttcaat
     5881 tgcttaagcg ccgcgcccat cacctcttcc gatttaccgc tggcatacac ttcagcgtta
     5941 tcaaaaaagt tcacacctgc atcgtaggct gcagccatca tatccacagc ggctttcaca
     6001 tccgcctgat tgctaaatgt aacccacgaa ccaaacgaaa gctcacttac ctgaatacct
     6061 gtcttgccaa gatgacgata cttcatgatg tctccattga ttttagaaac tcgcaacgct
     6121 ccaacattgc ggcacattcc gtccattata atcatcccat gcaaaaaaca ctgattcttt
     6181 gcgctatgct tctcagcgca tgtgcttctt caaatggtca accatcagac gggccgctgc
     6241 aaccatacct tacccgcacc cccgttaata catccacgcc aaacacgatc gtcatcatca
     6301 acacgcccat cccaaccgca accccattca cctacatcat tcaacaaggc gacaccttca
     6361 gcgaactggc tgaaaaattc aaagttacac aagacgatct gcaagctgca aatcctgatg
     6421 tctctccgaa tagcatgagc gtcgggacaa cgctcctcat cccgggcagt ttcctcaccg
     6481 cagcagacgc ctccaccccg acccctgtgc ctgctcctgt cacccaaagc atttgccacc
     6541 ctgccgcaga tggcggcctg tggtgttttg ccatgatcca gaacaactct gcgggcttcc
     6601 ttgaaaatgt ctctgcccag atcaccctcg acccaaacat caccgccacc gccttcaccc
     6661 cattggatat catcccgccg aattcatcca tgcctgtgta tgtctttttt ccaaatacac
     6721 ctgctgcctt gggggcaaat ccgcaggtgc aggtgttgag cgccattcaa ttgagcgcaa
     6781 atccgccgcg ctatctcaaa gcgaccttga acaacgccat cgcccaaatt gactgggatg
     6841 gccgcagtgc gcaactcagc ggagagatcc ttttgcccgc ggaatcaaca gccgccacac
     6901 aggtttgggt ggcggctgtt gcgtacgatc agttgggtca ggtggtggga atctcccgct
     6961 gggaaggcga agcgattcag gctgggggga gtctaaaatt caatttcgcc gtatccagcc
     7021 tcaggtccac gattgacgcg gtggattttt ttatcgaggc aaggtagatg aactttcttt
     7081 cctctttctt ctttcatcat gcaaagaaag aggaaagaag caaaattact tcccatcaat
     7141 ccataaatat ctttcaagcg gaacggcaat gggcgtaaac acatagccct ttacgtatgg
     7201 ctgcaccaac tcataggaaa gcgaatgggt ggtgaacaac acgggggcat catccacgat
     7261 catttgctcg gcttgctggt acatggcgat gcgtttggtc acatcctgtt cgatgcgtgc
     7321 ctgttcgagc agggcatcca acgcggggtt tgaatagccg ctgttatttt gcttcgaccc
     7381 gctgtggaat aaaacatcgg caaaattttc ggggtcggga taatcagcgc accacccgcc
     7441 gccaaagatc tgaccatgat tcccggaata gatctgctgg ttgtaaaagt tgtattcgat
     7501 attctccacg gtgagattca caccaaggtt ttgctcccac atttccactg tggcggccac
     7561 gctggaactg atgtagctgc caatgccgcc gttggtatac acaatgggag gcaaaccttc
     7621 cgggccgccg tatttggatt gtttgagtaa ttcacgtgcc tgctgggggt catagggcag
     7681 gcctttcaat tgataattga atccaggcag tccaggcggg aaagggccga aggcgggcaa
     7741 agcccgtccg cgcaaaacga catcaatata acgctggcgg tcgaatgcca ttgaaaatgc
     7801 cttgcgcaca ttgacatcgt caaaaggcgg ctgtgtcgtg tcaaagacca catagcctgt
     7861 gcaaagattg acacccgtca ccagttgatt gtggagcggt tcttcaggat cgagcatacg
     7921 gtccacgctg tagaggccca cacctgccac atccacatca cctgtttcaa agagacgcac
     7981 atcgtcgcct gcatacaatt tgacgatcac atagggaatg gacggcatgc cgaggtaaaa
     8041 atcttcattc gccgcataca ctttgtattc gttggacacc cattcagcaa ggcggaatgg
     8101 gcccgtgccg ttcgggtcgc gcacccattc ttcgccgctt tccacattgg cctgatccac
     8161 aacaaaggca gtggggtagg taagtttcaa aaggaaatat ggcttgggcg catcaatggt
     8221 cacttgcagt gtgcgttcat caatggcggc caacccgctg atatgctctg cctgacctgt
     8281 gttcatttca cgcacgccga caatatcgcc aagataggtc aatgccgtgt ctgaagccag
     8341 ttcagggctg acagcccgtt cccaggaata gatgaaatcc tgggcagtga cgaaacgtcc
     8401 gctgtggaat ttggcgtttg cgcgaatgtg aaatgtgtat accgtgccat cgtcgctgac
     8461 atcccatgtt tcggcgaggt caggtgtgag gttgagatgc gggtcaaaag aaaccaatcc
     8521 actgaagacg agtttatcgc ctgagctgta tgtggtggcg gggtcgtaat cgcgcaggtt
     8581 ggttgattcg ccgccgctgt acaccagggc ttgatcgaag ggaatatcca cttgaaatgc
     8641 gaacacatcc cttgcgctgg cggagaggat cagagagcag acgagaacca gcaagaggct
     8701 gcgaatcgca aatgaaccac gcctgaattt gagtgaaaat ggattagcca tttttacctg
     8761 ccttttgatt ttgagaacga gcaaggatgc cgataaccat tacacccaag atgagcagaa
     8821 tcacaccgca catgcaaatc agggcaatgg tcaatgcaat gggcgggcct ttggctatgg
     8881 gtggagtagt attgacgaaa gaagaggtgg gaacagaata gggtgtgggc gtaaccgcca
     8941 gaggcgctcc tgaaacgggc acataagtgg ggacaaaggt tggggttggc tgtgctgttg
     9001 gttgggcaga aacagttcga ggcgcagccc cgatcgcttc tctccaggcg tcttccaatc
     9061 catcagtgtc gaatccgtac acttcgatga gcgcatcgtc aatcggcttg gcatcgcgca
     9121 aggccgccag gatctcggtc atcttgtcct gcccataggt gctgataaga aattcgacga
     9181 tgctatgcga ctgactgtaa gaaaggtttg ccttgtcggg aagttctgag aagccgccgt
     9241 tcaacgaacg gatggtgagc agggtgtcat ttttgatggc gtcatccaac tgcgattgca
     9301 tgtaagaatc cagctcgcct tcgctgaaca tggccagacc ttcgttcaac catgtgggca
     9361 ctgttccaat gcaagagaaa gtgaaatgac cgacaagcac atgcgtcagt tcatggatca
     9421 ctgtgttctt atcccaattt gaatcagaag cggagattcc catgatgacg atgttgtgat
     9481 ctgagaaagc catgccgcca gtccatgatg gttcgtagag aatggcatca cgcatgtcat
     9541 cgtagttggg gtagacataa atatccacca catcttcaat ggacaggcct gcctgctccc
     9601 tgttgcggcg caggccttcc gtacctgcat caagcatggt ttgggcaaag gctttgtcgt
     9661 tgtcatacca gtgtacgcgc agttcgtcac tggaaagggt ctgccagtta tgaacgtcat
     9721 cgagccagat ggcggtcttt tggtcactga caaattcggc gccagattca tctgtgtatc
     9781 gccagcgcca ccagatcgtg gaaccaggcg gcagcgaacc gctctgacgc atatcccagg
     9841 tccactccac ttgcaccgtg gtggatgggt caaactgtgg aaacgccttc gcgatcactt
     9901 caccacaggt ttgctgttca tttccgtatt ccaatgtgac cgctgtgatg gcggcggccg
     9961 atttgagtgt ggctgaaaat gtggcggtat tcggaaaatc aaaaacaacc gcatcatcct
    10021 gaacctcggc acctgccgct gcaaaaacag actgcaccgg tacgacagcc agcagcaata
    10081 aaaccgacag aatttttctt aagtgcatct ttccaactcc ttggtttggg gcaattttct
    10141 tctttcaatg tctgcccaaa atataacatt aaccgctttt gccctgcgag ggtaccctgg
    10201 tcaaaggtca atcatcaagg caaaacaaaa tatcacatcc aaataagaca aattcgggaa
    10261 agattcatga catccatcag ccctcaaaac taagggtcgt ttaatgtcat catgtaaaat
    10321 ggatgtagat tgtgatattc ataattattc aactcaaaaa agcctgatcg gtctttaccg
    10381 aaaaagcgat tcgttcggag gaatccatgc aaaaaaacaa attcattctc gggggcttgc
    10441 tcatacttgc ggcagtggta tatctgatct tctcatccac ccaggcgagc gccgaatact
    10501 tcatgaccgt ggacgaaatc cacgcagaag gcgataccgc cattggcaag agcctccgcc
    10561 tttcaggcgc agtgctcggg gatacgatcc aatacgatgc agatacgctc accctcacct
    10621 ttgaggtggc gcacgtttcc ggtgacaacg ccgaaatcga agcgcagggc gggctggctg
    10681 tcgttttgaa ggatgcggtc aatgacccaa gccggcagcg catcacagtg gtttatgtag
    10741 gccccaaacc tgacctgctc cgcggtgaag cacaagccat tatgactggt acgctcggag
    10801 atgacggcat cttctatgca gacgaattgc tcctcaaatg ccccaccaaa tatgaggaag
    10861 cggttccaga acaggccgca acgaactaaa agaacaacct gacgggtgga tgcctgtcag
    10921 gttttgtttc taacacaaac tttcactgca cacaaaaaac aaaggaaacc ttatgttcgt
    10981 agaattcgga tatggcgttt tattggtaag tttcctcgtc gcaatttata gtatttttgc
    11041 ggcggtatat ggcgtttcca acaaatccac cgccatggtg gaaagtgcaa ggcgcgccat
    11101 gctgctcacc tttcccctgc tctccatttc agccgctgtg ctggtctatt tgctggtcaa
    11161 taatcacttc gaagtctcct tcgtttatga agtgaccagc cgcagtatgc caacctacct
    11221 caaggttaca gcctggtggg gcggacaggc cggttcgctc ctcttctggt cgtggctgct
    11281 ttcagcgttc gcttctgcgg tcaccatccg caagtgggaa cgtgatatcg aatttctgcc
    11341 gtgggtgatc gtgatctgct ccaccacgct ggtctttttc gttgggatgg tggccttcta
    11401 tgagaatcca ttcacccgtt tctggctggt cggtggaaat gtggagccgc acctctttgc
    11461 cccgctaaat gcgactctct tcaccgctga cgatggaaac ggcctgaacc cgcttcttcg
    11521 ccacccgggc atggtcattc atccgcccat gctgtatctt ggtttcgtat cattcgtcat
    11581 cccctacgcc tttgccatgg ccgccctcat caccggccgc accgatgacc gttggattcg
    11641 cctcacccgc cgctggtctt tgtgggcctg gctcttcctg tcctttggcc tcgtactcgg
    11701 tggacgctgg gcttacgacg ttttgggctg gggcggctac tggggctggg acccggttga
    11761 gatcgcagcc ttcatgccgt ggttgacggg taccgccttc ctgcattctg tgatgatcca
    11821 ggaaaaacgc ggcctgctca agcactggaa tatgattctc atcattctca cttatgcctt
    11881 ggtggtgttt ggcaccttcc tcacacgctc tggcgtactt tcctcggtcc atgcttttgc
    11941 caacagcccc attggcccgt tatttatggg gtttgtttcc atctcgttga tcgtatcgat
    12001 cgcgctggtg atctggcgct ggcctgaatt acgcagtgaa acagagatga agtcaatgct
    12061 ttcacgcgaa gctttgttcc tgctcaataa cttgttgttc atgagcgtgt tggtggtctg
    12121 tttttggggc gtgatcttcc cgttgatttc cgaactgttt acaggtcaga aagtgaccgt
    12181 tggccctcca ttttatgaac gtgccaccgc cccgctcttt gccgctttga tgttcctgat
    12241 gggcgttgct ccgctttctg cctggggaca ttccacggtg accacccttg gcaaggcgat
    12301 gtggaaatct gcggttgctg ccgtggtggt gacgggcatc ctgtttgcca cttacacaca
    12361 aaatgtcatc gcactggtgg gtttcttcct cattgcgttt gtcatcttcg tcaccgtgca
    12421 ggagttttgg cgcgccgccc gtgccaggca gcgggcacag aatgaaaact tcttcacttc
    12481 actcaccagg ctgatgggcc gcaatcgccg ccgctatggt ggttacatca tccacatcag
    12541 catgatgttg atggccattg gcatccttgg cattgagatc ttccagaccg aaacacaggg
    12601 ttcccttgca aaagatgaac aactgagcat ttccggatac accctgcaat accgtgaact
    12661 ggcttcatgg gatgaccgtg gcgaaggcgt aaattacacc cgcgccgtgg tggatgtgta
    12721 caaagatggt gaatacatcg gcgagttgaa cccgcgcatt gactattatt tcgactcaca
    12781 acaaaacatg accatccccg gcaaccgttc cacacttaag gatgatttat acgtcctgct
    12841 ggtggattgg cagcctgttt ccaacacggg agccaccttt aagatattcg tgaatccgct
    12901 ggtgaattgg ttgtggatcg gcagcctggt cttcatgctt ggcgttattt tcgccgcctg
    12961 gcccgaccgt gaccctgccg aagaacccgt gcgaagcgga gcggcacgca cagcccgcca
    13021 aaccagcgca gcagattgat cgcaagttag gtttcaggtt acaagttaca ggttgcaaga
    13081 aaaatcaacc tgaaatttcc aggagcatcg atgaaaaagc ttttttattc tttcaccttc
    13141 gcagttttaa tggcggccct gtttaccggc atttcatttg cgcaggatac caccccgccc
    13201 accgatgatg aagtgaatgc ggttgcccgc cagttgtatt gccctgtttg cgaaagcacc
    13261 ccgttggatg tttgtccgac cgaagcttgc aaagattggc gtgaacagat ccgtgtgatg
    13321 ctctcggaag gcaaatccga agaggaaatc ctcaagtatt tcgaagcaca atatggcgct
    13381 cgtgtactgg ctgaaccccc gaaaaaaggg ttctactggc tgatctattt gctgccgccc
    13441 gccatcatcc tgctgggtgc cgtcatcctg ctgcgctcaa tgaaacagtg gacgaccaag
    13501 cccgttgctg agtcaacagg catggacgag gcaggagacg cttcgtcggc tgtgaaagat
    13561 gattatgtgg cacgtctcga agaagaattg aagaaacaaa aataaattcc cctcatctac
    13621 ggccttgccg ggataaggat catggagaat gttatgaccg aagaaacaac gaacacaaag
    13681 cgcggcgtac cagtttgggt gcaagtcgtc atttgggctt ttctggtggg cctgttgggg
    13741 ctggtgggct ttggcctgaa ccgtgcccgc aaccccatgg ctgagatcgg caagcccgtg
    13801 cccgatttcg acattgtgta ttatgaaggc tacgaatacg acagccagcc gcagatgaaa
    13861 ctctctgacc tgcaaggcaa gatcgtcctc atcaacattt gggcttcgtg gtgcaaaccc
    13921 tgcgaacagg aagcgcccga gttggaagag gcatggcaat tctacaaaaa cagcaatgac
    13981 gtggtcttca tcggcgtgga ttatgtggat acccccgccg gggccaacga atacctcacc
    14041 aagttcaaca tcaccttccc caatgcgcct gacctgcaat ccaccatttc aagcatactc
    14101 aaccgccaga tgggcgtacc cgaaacatac atcattgacc gcgaaggcgt tttgcgccac
    14161 atcaagatcg gcccctttgc ttcagtagat gaaatcaagt ccatcattca aggcatcgaa
    14221 tagggggaat gacacatgga actaggctca atctttctgg tactcgctgt catcgtggtg
    14281 gtctttgcct atctgtatgc gccattcaca gcccgtgcgc gccgacttcg caacggagag
    14341 actcatcagg tctcctcgct caaagccgaa cgtgaccgtg tcatcaattc actgcaggaa
    14401 ctcgatttcg atttcaacct cggcaagatc cccgaagggg aataccccga acaacgtgca
    14461 gcactcctgc aaaaaggcgc agacattctc cgcaaactcg atgaactggc acctgtctcc
    14521 tcctctgcac tcaacgccga agcacgcatc gaaaaagcca cggctgcagg ccgcgccgat
    14581 tctgccgccg tatccgccgt tttggatagc gacgacgata tcgaagccat gatcgctgaa
    14641 cgccgaaaaa gccacaaaag caaatcggct ggcttttgcc cgaaatgcgg caaacccgtt
    14701 ttatccgcag ataaattctg cccttcctgc ggcaaagctt tgaaataact ggtgatcggc
    14761 aagcaatcac caacccatcg aggaacttaa tgaaactacg tcatatcgtt tttattactg
    14821 caatcgcgat cttactgacc gcctgcaact tcacactcgc agccgatgtc accccgccgc
    14881 ccggttatgc ctcccccacc cccatgccca ccctgggtcc gctataccct gccagcaatc
    14941 ctgatattca caacggcgcg gtcatctaca tggaaaaatg cgcaccgtgt cacgggaatg
    15001 caggtttggg cgacggcccg caaggcaagg aattgcccgt tacagtagcc gccatcggcc
    15061 tgcccgccac agccacaaaa gccaaaccct ccgactggta cacccaggtt acacaaggca
    15121 acctcgaacg cttcatgccg cccttctcca gcctgagcga tcaggaacgc tggaacgtgg
    15181 tctactacgc gttcacgctt cacattctgc ccgagcaggg cgatctcggc cagaccctct
    15241 tcgaagagaa ctgtgccgat tgcgaaaaat atttcagcga cctcaagacg atgtcggcac
    15301 tttctgaaga cgaaattgtg cagatcatca aaaatggatc gggcgaaatc cccgcctttg
    15361 gaaaagattt cagcgacgac gaagcccttg ccgttgcagc ctacctgcgc actgtgatct
    15421 tcgccccgcc tcccgctcaa ccgacacctg ttcccgcctc cgaaacgcct ctcagcgccg
    15481 aagcagggac tccgtccgct gtggaaacgc ccaatggcac cgagcaggcc caaaccacgc
    15541 cagaagcgga taattcattc ctcaccacgt ttgggaatat cagcggcacc gttgataacc
    15601 aaaccggggc agcgttgcca tccaacctca cggtgaaatt gcgcgccttc gagcacggcg
    15661 gcgaccccag cgccgggcca gaggaagtgg attcaaccga ggtcaccatc aacgccgatg
    15721 ggtcctatca attcgaaaat atggaaattc cagaaagccg catcttcgta gccgagttgg
    15781 agcttgaagg catcagctat caaaccgatt tcgccattgt ggaagctgga atgaccagtt
    15841 tggttctgcc gccgctcacc gtgtatgcaa ccacggaaga tctttccaca ttgaagatca
    15901 acgaagcgca gatgtacttt gattttgcca acaccgaaga cgtacagatc ttcacggttt
    15961 attcgttcac caacactggc aacaagacca tcctcgtcaa catgggcgcc gatcaggtcg
    16021 tgccgttcat cgccttcccc aaagatgcca aagcccttgg ctatgaagcc atgcaaaatt
    16081 ccgccgcttt tgtacccact gatggcggtt ttgccatgcc ccccagcgag ctaccctacg
    16141 gcctgatcgc atttgcctct gtgcccaagg ccagggaagt cgccatttca caatctgccg
    16201 tgttggatat cgagagcatt tccctctacc tgccgcaagg catggaagcc gaaggcgcaa
    16261 ccctcagcga tgctggcttg caaaacatgg agaccatgag tttccaggtc tattctgcaa
    16321 cggggctcaa gaagggtgaa agcatcgaat tcactgtcag tggagaaccc tccgctacat
    16381 ctgaaactgc cgacctcacc caaaaccaaa ccctgttgat cggaatcggc gcattcggtc
    16441 ttgtcctcat cattgcaggc gggtggctgt acatccgtga ccgcaagaag gaagaggatc
    16501 ttgacgatga agatggcgaa ttcgaagaca ccgaatccac catggacgcc atcatcgccc
    16561 tcgacgacct tcatcgcgaa ggcaagatct ctgatgaagc ctaccaaaaa cgacgcgctg
    16621 aattgaaaag cgcattgaag aaacaaaaat aaaaaaaatg agacggttga aagttgcaag
    16681 tggaaggcac caaattccac ctgcaacttt caacctgcca ccttcaaacc ctactcacat
    16741 gatcgaagta aaaaaactcg tcaaacgctt tgggctaaaa gtcattctgc gcggactcga
    16801 ttttcaagtg cagcctggcg aatttgtggc attgcttggc cccaatggtg ccggcaaaac
    16861 cacctttttg agaatcctcg cctcactttc ccgcccttcg ctgggtgaag tgagagtggc
    16921 aggctacaaa cttccgcatg aggccgctca agtgcgtgcg cgcctgggtg tggtctcaca
    16981 tttgccgctg ctctacccag acctcaccgc agaagaaaac ctgaggtttt acgcgcggat
    17041 gtacggcatt gaagatcagg aagcccgcat caccgaagtg ttgggcatgg tggggcttga
    17101 gccgcgccgc agagaccttg tcggtgcctt ctcacgcggc atgcagcaac ggctcgccat
    17161 cggacgcgcg gtcttgcatg accctgaagt gatgctgttc gacgaaccct acaccggcct
    17221 cgatcaggat gcctctgaaa tgctcgatga ggtactgcgt tcggtcgctg cgaaaggccg
    17281 cacagtggtg atgacctcac acgatctcgc ccgcgccgaa gaactggcca cccgcttcga
    17341 tatcctttcg cgtggagtga tcgccacctc agccaaacgg gaagacctca aatccaccaa
    17401 cctgctggct ttttacaaac aatcactgga atcatgatga aacgttcatc cccatccctc
    17461 ctcaaagcca cgctggccat cgtacaaaaa gaccttgccg cagaattccg ctcgcgtgag
    17521 ttgctctccg ccatgctggt cttttcgctt ctggtcatcc ttattttcaa ctttgccctg
    17581 gagttggatg tcaaagtacg gcaatccgtc accgccggtg tgctgtggac cacattcgct
    17641 tttgcaggca cgcttggcct taaccgttcc atggctgtgg aaaaagatcg cggctgtatt
    17701 gatggcctgt tgctcgcccc ggtagaccgc tccgccattt attttggcaa ggccatcagc
    17761 aacctcgcct tcatgttgat tgtcgaagcc atcgtgctgc ctgtgtacag catgctatac
    17821 aacatcaacc tctttcaact ggatctgctc ggcgtgatcc tgctgggctc cattgggtac
    17881 acggcggtcg gcaccctgct ttcagccatg gcggtacaaa cccgcacccg tgatgtgctt
    17941 ctacccatcc tgttattccc catcgctgta ccggtgctgc tggcagcggt gaaagcgagc
    18001 ggtggaatgc tggccggagc cgagtttgca gagattctcg ttccactgaa cattctgatc
    18061 gtatatgaca tcgtcttcat cgcggtggcg ttcatggtgt tcgatttcgt tgtagaagag
    18121 tgaacgagac agcaaatcag agattcagcg agccaaccat ggattggcat tcgtaaatcg
    18181 caaattttag caaggagttc atatgcaacc aaaaccaact gctctcaaag tactcgatat
    18241 tgcatccatt attgtgctgg caatatccac ctatcttgcg cttttcttcg cccccactga
    18301 actggtgatg ggagacgtac aacgtgtctt ctatttccac attggcacag cctgggtcgg
    18361 tctgctgggc ttcattatcg cagcaggatc aggcatcatc tacctcatca ccaaagacct
    18421 gaaatgggac cgtttcgaag tggccgctgt ggaagtgagc acggtcttct tcttcctgac
    18481 aattgttctt ggttccattt gggcccgccc cgcctggaac acctggtgga cttgggatcc
    18541 gcgtctgacc actgccgccg tcactgagct gatttatgta gcctacttta tgctccgtca
    18601 gggcattgac gatcctgaaa aacgcgcacg tttcggcgcg gtatacaccc tgttgggcgg
    18661 aatcagtgcc ccggtcacct tcatggtcat tcgcctgttc cgctccatcc accctgttgt
    18721 catcggcgct gccaatgcaa atcaggaaaa aatgagcatg acccccgata tgcgtgtggc
    18781 cttcttcttt gccctgtttg cctttaccgt catctttatt gaccttatct ggcaccgcct
    18841 tcgccttgga agcatggaag aaaaggtcga acaactcaag ctcaaagttt caatgtaaac
    18901 ggagaaacca caatggaatc cacacccgat acttccagct atatgatcgc aggctacgtg
    18961 atctccttca tcaccatggg aatctacgtc gccagcctgt atatccgcaa taacaacatt
    19021 ctgcgggata tcgaaaccct cgaatccatg caagaagaaa agccaaaagc aacgaaaaag
    19081 taacagccag gaatgacaga gtccgcttat aatataggcg gactctgttt tttgatgaat
    19141 atgacgcgcc gctggatgct cattttcctt gccttgaccg ttgcctgttc aggcgcggtt
    19201 tttacgcgcg atatttccga agcactcggc aatggcttcc agcctgtttc tgccaataat
    19261 cccccgcccg gcccagaccg ctatacctat attaccgtcc aatacactgc ctacgaatgg
    19321 tatttggcaa cctggaaaga tcaagttccc ctctgttcac tgatcaccga ccatgaaggg
    19381 atgcccctgc caggcgaggt gtaccgggac tgcggtgaaa ccatctacga aaagtggatt
    19441 gtgcagaagc catgcatggc gaccaataaa agatcctgca ccggcttcta tatttccccg
    19501 gtaaagaatt accctgcaga aaaagaagtt cctgcagaac ttgaacctgc cacggtttgg
    19561 gtctcgttgg aaggctgtga aactgtatta agtacatcga cgaatttttg cgaagtggca
    19621 cccacgttgg tctttacggg tcaggaaccc ctgcccggcg aaaccatcat ccgcgtagaa
    19681 gggacatacg atggggcgcc tttcaaatgc gaggggacaa acgtatgtaa attcgaaatc
    19741 ccctctacag atgaggatgg agcgaaaatc gaattttggg ccttttccag ttatggagat
    19801 agcagcctca tttacagtgc ccagattcga gtccaaaaag tggatgaggg tgaccctgat
    19861 caattgtatt ggtatgccga tgtgctttcc acacaatgga caggtgaagc agtggcaacc
    19921 tgctccggag cctggggggt cttcccaccc gttggcggac cgcccacctg gttgaccaca
    19981 cccaaatata gcgaagaact cagcagtgat attccctaca cctatcttgc cgccaacctg
    20041 attcgtcagg gggttgtgga tgccagttcc tgtgcagatg gtggtctcac gcccgaaggt
    20101 ggagtcaacc aatgcgggtt ggaagcagcc cgccctgcag tgacacaatg gcagaaccag
    20161 ttcgatgagt tgatcctcac tacggcgcag caaacagctg tccctgcgcg attgctgaaa
    20221 aatcttttcg caagagaaag ccagttttgg ccgggcagtt ttcagggtat ggatgatacg
    20281 ggtcttggtc aattgacaga aaatggcgca gataccaccc tgttctggaa tagttccttc
    20341 ttcgagcaat tttgtccctt cgttttatca gaagaaacct gcggcaatgg ttatgtcaac
    20401 atcaaggaag aggaacgggc ggaattacgc cgcgcactgg ttggcagtgt gaacgcaacc
    20461 tgcgagaatt gtccattgcg cattgacctg ccacaggcaa attattcggt gggggtcttc
    20521 gctcattcga tgatcgccaa ctgcgagcag acgggacaag tcatcaaaaa ttacacaggc
    20581 cagaatgccg gtgaagtggc ctcttatgaa gacctatgga agtttacgtt ggtcaactac
    20641 aatgcaggcg ggggctgtct ggcagaagcg cttacttaca cccttggctc aagcctgccg
    20701 cttacctggg aaaatgtatc gccgttcctt gcaggggctt gttcaggcgc agtggattat
    20761 gtaaacgaca ttagcaagta aaaaaaacag acctaaaaca ccccgaaatt aatctttcaa
    20821 ttctttggcc gcgaatttac acaaaaaagc cgatccattt tacagggtcg gcttttttta
    20881 tatctcgtaa tattttgcct aagttgcaag aaatattttt aaaatgtcat tttgaaccac
    20941 ggagacattg aagcagtgcg gggcctcctg cggacggagt tcttatttgt ttcctctgtg
    21001 cctccgtggc tcagtggtta atctttttgc aagaggtcca ttcaaaattc tcggtggaat
    21061 catgtctctt tgcttaaact atcgaaatat tcccagcggg ttgtcacttg gagaaaaccc
    21121 cgggaagacc gcttcgagat ttgaattccc caaccgtgta gatagcaact cactcaaaac
    21181 ctgacggtaa tcggtggtga tcgcaagatc ggcatggtcg tacagttgat cgttattcaa
    21241 cccgggccac tgaccataca cccgcccgcc gttgacgttt ccgccaagcg ccagcattac
    21301 cgagccgtga ccgtgatcgg tgccatagga ttcgttttgc acaagccgcc gcccaaactc
    21361 gctgatgacc accacagaaa gtttattggt gtaacctgaa tcaaggtcga gataaaaatt
    21421 ggaaagcccc gaagccaact gcccaagcag atcgccgatg tagccgccgt ttccatcggt
    21481 ttcgtattcg tgggtgtccc agccgccaaa atccacggct gccacacgca tccctgcatc
    21541 aagtttgatc atgcgggcga tcgttttcaa ttgctgaccg aattcatcgt cgttgtagaa
    21601 tgcgccgttc gcgggttggt attcctggct gaccaacggg ctgacaatat cgagcgcatc
    21661 aagcgtgcgc aagcctgcgc ggtgcaaaag cgtttcgccc tgatacatct gccgcaaggc
    21721 ggtttgctgt gaaccgatca aatcattgcc ccattgagag aaatcttcag gcgtgctcac
    21781 gttgacggtc gggacaaaat tcaacagcga agtgggctgc ccggttgtgg aaaccacagg
    21841 caaaatggag gaaacccctg tctcctgcaa gtggcgcgtg atccaacctg aggtggtatt
    21901 cttcgagccc ggcgttccca actcgatgta ttcctgtgca tcgaagtgac tgcgcgtatc
    21961 gtggttcagc ccaacggcat gaaccacacc cattttgcca gcctgataca actcatgcag
    22021 cggggcaagt gcagggtgca aaccgaactg gtcgttgagc gaaagcaaat ttgagatctt
    22081 gatatcgggg cgggcttttt cgtaaaagcc gcggtcatcg ccttgcaatg gcggcaccac
    22141 attgagcgca tcccaaccgc cgcgcaaaaa cacaaccacc agcgtatccg acggattatc
    22201 ctgttttgca aaggcgaggt tcgtcaaacg cgcgcctgcc atcgaggcaa tggcagcgga
    22261 acaaccttgt aaaaactgtc tgcgggagat tctaggtgcg ggcatggttt ctcacttcca
    22321 ctgaaaatcg gggctcatca aaatcacttc caccattgca ggcagcacat acgccacatg
    22381 gtcttcgggc aacagttcct gcgtcccgta ttcctgtgcc atcacagcga tgacggaatt
    22441 tctatcggca tcggaggaaa gcggacgacc gaggatgcgt tgaatccaaa aatcagccag
    22501 cgactcggcg gttcggatct ccgccggggt ctggttcaac aaatctgtgt gcagggtcag
    22561 ccccttgtca tcgtcattga gccagttttc agccaccccc acagcgaaat tccagcgata
    22621 caaaagggac atggaatttg cccacgcctc cttcacatcg gggtaaccat cgggtgaacg
    22681 gcgttcgaag agcggctgac ccatcaggct cagcatccac ggcacgccgc cgggaacacg
    22741 ggtgaaatcg gaattcaacg accgcagggt cgaaatacaa gcctccagcg ggcgcttgat
    22801 cttctgtccc caactctgct tgaattcatc agagaggagc aacacttcca tcacacgctt
    22861 caactggtcg ggcgcgtcgc ggaattctgt aaaggcggcg gccactttct ctacaacaga
    22921 atcaggcggc aggtcggcga tgaagcggcg gcataatttc cgcgcaatat ggcgggcagt
    22981 gcccggatgc gccgcgagca agtccagcac atcttttcca tcctgcatat cgggttgatc
    23041 ggcagggata tattttccaa gcaccagttt gttgaaacgg tcgtgccagg gtttgtaata
    23101 gatgaaattg cctgtgatgc caacaccctc ctcccattcg ccgaggtcat catcaatgcg
    23161 ccagcccgtg aggcagcgcg cggcttcgta cacatcgtta tccacatagc cgatggcgat
    23221 tccgttcgca tctttttcta ccgtattcgg gtcgcgcaca ccgagataat tttctgcgcc
    23281 gagggtgtgc agttcaaaaa gttcacgggc aaaattttcg tttgggccag catcactgct
    23341 gttgttttga ttcaggtaat acaacatgga gggatgggat gccaccgctt cgagcatctg
    23401 acggaaattg ccaagcatgt gtcttcgcag cacatcgcgg ttgtatgaaa ccagcaatgg
    23461 cacgccatca tcctgccaga agaaaatgtt gaaatggttg tgccagaaat cggccagcaa
    23521 ttcaacaagt tgttttttgc tgaacacagc acggagcagg gttgcatcca caagttcata
    23581 ggcaggcaga ctgtaccact cccaataggc atcatcattg gcatcgtagg cgttgttggc
    23641 aatatggtcg gagtaaagtt gttcgtgtga tttgtgcaat gcctgaaagc ccgccgcact
    23701 gtaacgggat tcaaaatcgc tgtcgtcgag cgaatcggga ttcagttgct gttgcacaaa
    23761 cgcccgcaga cgctcatcgt ccgtgctgcc aagcgcgttg aaggcttcga tatccccagg
    23821 gcgaatcccg aaggtcatgc gcccaagcgc aaggatcgcc agcgaaacag gcggcaatga
    23881 aaccgcaggc tcagggatgg aaatatcatt tgcgctggtg gtgggagaga gtggagcagt
    23941 ggaggtttct tccccgggtt ggcaggcggc aagcagggaa gctgttgcaa gtgagcctcc
    24001 caatttgaga aaatctctgc gtgaaaattg ttttgaattc aagacgcctc ttttctcttt
    24061 cgttctacaa agcaaacact tcacgaaatg cgcgggtcac ttcggctttc acctgttgca
    24121 tcgaaggcgc ggggtcaagc aggtcagcca gcgagacaac aggctcattc aagccgcagg
    24181 cgatgatgcc attccaatac tccatatcgg gctccacatt caacgcaaaa ccgtgacggg
    24241 tgatcccgcg tgcatccact ttgacgccga tggcggcgat ctttgcaggc tttttgcgat
    24301 cttccggcga acagcgcggg cagcgcgaat gcacatcaga ttggatccaa accccggtca
    24361 accctgaccg ttggcctgat acaatgccaa aatgcatcaa cgccgtaatg agagtctcct
    24421 ccaacttgcg gacgtagccg acataatctg cttcaggcag tttattttca ttatttaatt
    24481 ttccgagtga taacagggga tatcccacca actgcccggg gccatgataa gtcacatcgc
    24541 cgcctcgatc cacccaatgt gtggcgatgc ctttttgttt cagttgttct tcgttccaaa
    24601 gcagattttc ggcatgtccc tttctgccga atgtataaac gtgagggtgc tcaagcagca
    24661 agagcgtggg cgggcgtttc ccctcagcaa tttccgccgc ataatcatcc tgcaacttcc
    24721 aggctttttc atattcgatc agccccaggt cttcgatggt aaaagggaac atataagaat
    24781 gaaaacaata aataaaaagt tgaaaatgtt tttgaacgaa tttggaatta actgaatatg
    24841 cgacggtata aatctgattc gagcaggccg ttcgggtcgc agcgtttttt cagtttcctg
    24901 aatttttcca cggtctcttc gccatagaag gcccgtgcag tttcggcact ggtttcgctg
    24961 ttcttggcaa aatagaaccg accacccgca tttagcacga tcttgtcgaa atcctgcagc
    25021 atggcactca gccttacgcg agtccgattc gtcactttga aatcgagggc cagggagaat
    25081 ccgtccacag catgggtgag caggaattta tcggggcggt gccgcttggt gacaccgagg
    25141 taggagggga gtccacgctt tttggcgagg gccagcatct cggtccatgc ttgatgcgcg
    25201 gtttccttcg gaaggaatga ttgatattgg atgagtccgc ctttgccata ggaaagttcc
    25261 cagagcggga cataatccaa aaggaagtga aaggcggcgt ggctttgacg gaaggtatgt
    25321 ttgcgcaggc tggaaatata tttggcggtg ttaaccagac cccagccaag attatttgca
    25381 aacggggtca tgaaataatg cagcatggat ttcggcatca cgccaaacaa acgatctggc
    25441 aggtgttgat aagaaagctg catggtcttt tgcgcttcag gatcttcgcc ttcgtgcagg
    25501 taatttgcag catggatctg accatgcccg atggtgttga tcgtatcaag ccagccgaca
    25561 atgtaatcgt aattcggggc gccatcttca agcgaggcca gatgatcctt aagtgtgtgt
    25621 gaaggccagg cgtgaacttc gagcaggcct gaatgcatct tcttcatttg cattgtgacc
    25681 gaagtaaaaa tacccaacat acccagcccg ctgatcatcg aatggaacag gtcgccattt
    25741 ttgtttgggg agcaggttac ttctgcgccg gtgggaagca cggcggtaaa ttccaagacg
    25801 tgttcaccga tcgtacccat tctaaaatta ttcttgccgt ggatattggc cgaaagacag
    25861 ccgcccaaag tggtgaacat ggtgccagag accacaggcg gccaccagcc atcaggcaga
    25921 accttctgcc ataattgttt gagcgtaacc ccaggctcgc accgcacctg acctgtggca
    25981 gggttccatt ccagaatctg attcataccg gaagtatcca gcacaattcc accgccattt
    26041 aaagcagcgt cattgtagct gcgaccaccg ccgcgtgctg caaccgtaag gcccatctgt
    26101 tttgcagaac ggaaggcttc gtaaatttct tcggattttt gaggtttgat gagataggag
    26161 ggagctttga gcgaatgccc aaagttttcg agttgggtaa aagtattgtt catattttct
    26221 ttgaatacac cctcaaagtg aagggccctt tggggtcggc taaaaggaca ggcggcggaa
    26281 gatcaccgat ggcacatgtt gaatggcaag catgatccag cgccagatgg atgcggtgta
    26341 gatcaattgc ttccgcttgc gcatggcttt gtaaatatct tcagcagctt gttcaggaga
    26401 gatcgcaaag ggagtgcccc cctgtgcggc cttgagcatt tcggttttga caaagcccgg
    26461 cttgacggtt aatacattca caccgtggcg cgtcaggcga ttgcgaagcg cttcgaggta
    26521 ggtggtcaat gcggctttgg aggtgttgta accggggttg ccaacacggc cacggtcacc
    26581 agccactgag gagattccta cgatctgtcc cgctttcgcg ctttggaaca tctcggctac
    26641 cgggctgagc caggccatgg caccaatgag gttgacctcg atcatcttgc ggtcaccttc
    26701 gaaattgtaa ctgttcaaac cagggggaaa gttgacaccc gccatgaata cgagcagatc
    26761 caatccgccg agttcggcgg taatttttcg caaaaggttg ggaacctcgt cgtagttgga
    26821 aacgtcatgg gcgtacgcaa gtgcacgggc ttcgccttcc gcagcattga tttcggcaca
    26881 aaggctggca agtttgtcct gtctgcgggc gatcagggcg agggtgtatc cctctttggc
    26941 aagtttacgg gccagagcag cgcccattcc atcagaagcg ccaacgatga ttccccgtcg
    27001 gcgcggatta agtggtgttg cttgagcagg cttgggtgag tttgacacgc gagtcctcat
    27061 aaaaacaata tggatgttat tctaacacaa tccaaatctg agaaaaaaag gctatataat
    27121 cgggatattg gcggtcattt tagaggatac cagccatgaa agaaacggtc ttctctgaat
    27181 atgaactgga tacatgcagg tttgtcttgc gaattccagt acaaaaggac ggttgccccc
    27241 cgcccatcgt catgataaaa aggtgttcac tatggctgga ataacggaaa aacaaaaaaa
    27301 tatctttcac catgcacggg agatcatcaa acaggatgaa tcgatcccgg aagaggagaa
    27361 agagggcatc ttcaagttga tacgggtgct tgtagaggtt gcagacctgg caccttccaa
    27421 actgccaaac caggaagaat cccccaccgc cttcacacag gaaatcattt cccgccatgc
    27481 attaatggct gctgtaaaac agcagatcga tgagcttgag tctcttaaaa aactcagcct
    27541 gaatgtgact tcaagccttg acctgcaaac cgtgctggat acggtggtcg cagaagccat
    27601 gaggctggta aaaaattcac gtgcggctca catctttttg tattcacacg gaaaactgga
    27661 attcggttct tcactcactg ctgaaggctt aaaaaacatg ccagtggcca tgccacgcaa
    27721 agaggggtta accgcaactg tggtggcgag tggagttccg atcattgtgg aagatatcac
    27781 cgaccatcct ttgtacacca atgtttccca aaattggcgc ggttccatca tcggcctccc
    27841 gcttaaatac aacaaatcca ttgtgggtgt gatgaacctc tcacgctcca cagtcggtgg
    27901 ttttacacgg gcggagttac gcctgatcgg gctggtagct gacctggccg ccgtggccat
    27961 ctccaatgcc agtttgcatc agcaggtggc cgcgcaagcc aatacggata gtgttacggg
    28021 cctgcccaac cgccgcgcgt tggacgaacg tattcaagag gagatccgtt ttgcaaggcg
    28081 aatgaacagc gaattctccg tagtcatgat ggacttggat ggtttcaagg ctgtgaacga
    28141 cacctacggc catgtggttg gcgatgaatt gctgtactct ctctttaatt atcttgcaga
    28201 gaacatccgc cccactgatt ttcttgcccg gtacggcggc gatgaactga ccctggtcag
    28261 ccgtgaggcc aatcaagata tggcggaaag cgtaacacaa aagatcattg acctcgtcag
    28321 agggtacaaa cccgcatttg ctgaaaagaa gaacattcaa ctcggaatta cagcaggcat
    28381 tgccatttat cccacccact ccaaaactgc aggtgacttg ctccgtgccg ccgatgccgc
    28441 cctgtaccaa gccaagaaac accatcgcgg ctcctattcc attgcgaaga tcactaccgg
    28501 tccgctggat cccatcaccc tgccccgcaa aaaaccagaa taaaaagatc caaaccgacc
    28561 gactcacaaa atcgtcctcc tgcaaccaaa cagataaaga tggggttata ttggtaaatc
    28621 caatcacggg aggcagatat gccccgcaaa gtaaaactca tcctcaaccc catggccgac
    28681 atgggacgcg cctggaaaac cgccaacgac ctgcgcccga tcgcacagga attcaaaggc
    28741 gaattgacct ggagtggaac ggtctacccg acacacgcca tcgagctcgc ccgtcaagcc
    28801 gctgaagaag gttacgacct cgtcatcgcc atgggcggcg acggcacggc acatgaagtg
    28861 atgaatgggc tgatgcaagt ccccgcagaa aaacgacccg tgatgggagt cgtccccatt
    28921 ggctcgggca acgacttcgc ctactccatg ggcatcaccc aaaaatcggc acacgccctc
    28981 gcgcacatgc tcaaagccga aaccattcaa agcattgata tcggccagat gaccgacgag
    29041 cacaaccgca aagaatattt cgacaacaca ctcggcatcg gcttcgacgc cgtggtcacc
    29101 atccgctcgc acaaactgcc catcgtcaaa ggctttttga tgtatctcac ggcagtcatt
    29161 caaaccatca tcctcaacca caaccccgca cacataaaga tcgaaaccga atcagagcaa
    29221 tgggaaacca gcacactcat ggtcacgctc tgcaacgggc cgcgtgaagg cggcggcttt
    29281 atgctttctc ccaactccaa aaacgacgac ggcaaaatgg aatacgtcat cgtcaacaag
    29341 gtctcacgcg gcatgatgtt ccgcctcgta cccgaattca tgaacggcac acacatgcgc
    29401 ttcaaacaaa tccgcatggg cgaattcaaa aaatgcagca tcacctcaga tcgtccgctt
    29461 tacatccatg ccgatggaga aatcttcacc agctttggca gcaacctgcg caaagttaat
    29521 ttcgaaattc tgccgcaggc attgaaggtg gtacgcggtt aatcactcgc tactttccac
    29581 tttctccttt agaatatgaa aattggaaag tagaaatgcc aagcctttac caaacctttc
    29641 tcaaacacga cctcggacat cttcgcatcg tagctgatct gtggggactc gaactcgaat
    29701 cgcaagatct ggattcagct gccgaagaac tctccgcatc tttgctggac ctggaagctg
    29761 tcactgaaac cattgacatt ctctcccctg aatcacgcgc cgcgttggat gccatcgttg
    29821 aatccaacgg caaaatggaa tgggcagcct tcacccgtgc tcatggcgaa atccgcggca
    29881 tgggcgccgc caaacgtgac agggaacgcc cacacctgaa acccatctcc cctgctgaac
    29941 atctctttta tcgcggcatc cttgcccgcg catttttcga atcgagcaaa ggctcgcagg
    30001 aattcgccta cattcccgat gacctgttgg aagtgatcga aatgctcgtg gtggaagatg
    30061 acgaagcgcc gacacccgcc ccgcatggcg aaccgcttgg ccgacccgcc acgcccgtgg
    30121 aaaaagccgt tgaactcccc gccaccgatc acatcctcga tgatgcaacc acgtatcttg
    30181 ctgccttgcg catcggcagt cgggcagccg aaggcgtatc aacgcgcaga cgcgaactgc
    30241 atgaactgct cgaggcagcc catctcatca aagatgacgc cctgcagcca gaagcggtaa
    30301 aaaactttct cggcgcatcc cgtgcagaag cattaaacat tctctacaaa gcatggtttg
    30361 tctcaaacac attcgatgaa ttgcgcctgc tcccgggaat tctctgcgag ggtgaatgga
    30421 gcaaccagcc gcaggtgaca cgcgagtttc tgaccaacct gatcaacgac atcccgcaaa
    30481 acaaatggtg gagcctgccc gccctgctgc gcgatatcaa aaccaaatat cccgattttc
    30541 aacgccccgc aggcgattac gactcgtggt tcatcaaacg cgagtcagac ggtcaatacc
    30601 tgcgcggatt cgcctattgg gatcaggtgg atggcgcatt gatcaagtat ttcgtccaaa
    30661 ccctgcattg gttgggcagg gtcaatctgg ccgccccaga agagggaaaa gagcccaccg
    30721 cgttctttct gctttcttcc tttgctgaaa agaatgaaga aaagggaaag gtcattgttt
    30781 cttcaaacgg aaagatcacc atctcgcgtt atttctcacg cgcagtgcgt taccaaattg
    30841 cccgcttctg cgaatgggac gaggaaaaag gcgacgaata tttttatcgc gtcacggcag
    30901 gttcactgaa acatgccaac gaacagggat tgaaatcgga gcagttactg gctttgctgg
    30961 tgaaatacac caacggcaat gtgtcgcctg cgttggtaaa ggcgctcaaa cgctgggaca
    31021 cgagcggggt cgaggcccgg gtggagagtc tgcttttgct aagagtcagc aggcccgaga
    31081 ttatggaaga gatccgcaag tcgaaggcgg ggaaatatct gggcgaagtc ttaagcccga
    31141 cggcggtgat cgtcaaaagc ggagccatcc aaaaagtaat ggcggagttg gccgagctgg
    31201 ggttactggc agaggtgact acgcatgaat aggggcaggc gaatcatttt gatcgttgct
    31261 gtaattctgg cattgattcc cgtcccgatg acatgcgggc acatgtttta cggctgtgcc
    31321 accgggccag atatgagcgg gaattactac acctattacg aagttgagcc tttgggaatc
    31381 acgttgatcg aaaccctcat tatgaccaat ttgaaaatct attactggag cggaaaagag
    31441 gcgcatttca tagggattcc atgacacatg acgcggggga aacgcatata ataaaaatgt
    31501 catttcacaa gcaatcagga gaaaaacaaa tgtctaaaac cgactggatt cagcaggaaa
    31561 ttgataattt gaaatcacag gggttgtata acaacatccg taccatcggc tcaccgcaag
    31621 gcgcatggtt gaccgtggat ggaaaaaagg ttttgaactt ctgctcgaac aattatctgg
    31681 gattggccaa tcaccccaca ctgaccgccg ccgcaaaaaa agcgaccgat gaaatgggcg
    31741 ttggccctgc cgccgtgcgt tcgatcgcag ggaccatgac cctgcatgtt gagctggaaa
    31801 aacggcttgc acaattcaag ggcgtggaag cggcgatcac atttcaaagc ggtttcactg
    31861 ccaatttggc caccgtgcct gcgctggtcg gcaaagagga tgtgatcttt tcagaccgtt
    31921 tgaaccatgc ttcgatcatc gatggctgtc gtttgtctgg cgcgaagatc attgcctatg
    31981 aacataacga tgtgaaatct ttggaagagc agatcaaggc gaacctgagt caatatcgcc
    32041 gcgctttgat catcaccgac ggcgtcttca gcatggatgg tgacattgcg cccctgcccg
    32101 atatttatga agtggcgaag aaatacgaca ttttattgat ggtggatgac gcgcacggcg
    32161 aaggcgtgct tggcaaaggc gggcgcggca ttgtagatca cttcggcctg cacggcaaag
    32221 tggatgtgga agtcggaacg atgtcgaagg catttggtgt ggtcggcgga atcgtggcgg
    32281 gcaaatcggt catcatcgaa tggttacgcc aacgcggacg tcccttcctg ttctcgtcgg
    32341 cggtcactgt gcctgatgcg gcggcctgtc tggcggctgt ggatctgctc gaagattcca
    32401 cgcaactggt ggataaactt tgggacaacg ccaagtattt caaggccgag atgaagaacc
    32461 tcggcttcaa cacgggagtc agcgaaacac ccattacccc gatcatgctc ggtgaagcgc
    32521 cgctggccca gcaattcagc cgtgagttgt tcgaagcggg cgtgttcgcc atggcgatcg
    32581 gattccccac cgtgccgcaa ggcaaggcgc gaatccgtgt gatgatctct gccgcgcacg
    32641 cgaaggacga tctcgataaa ggtctggaag cgttcaagac cgttggaaag aagttgggtg
    32701 tgatctaatc acaatggatg caaagagacc gcaaagtgct ttgcggtctc ttttttttca
    32761 ggtgacgtat aatttggagg atgtgggtat cagactgaca taaatggtca gcgattattt
    32821 ggaggtaaat aaaatgaatg ttcccatctt ccctgataat atcaatctct cccgtccatt
    32881 tcacttcaca accgagaatg cagacagtaa agcgaggatt ggtggcaata tgcccgaaga
    32941 attgtccatc tccctcaatg aaaatgccca atatttcggg acgttccccc tttatgacaa
    33001 cgagtcacct ttatattttt ccctgtttat caattgttcg tttaaagagt tcgttgtaga
    33061 ttggcacggt agaatcaaag atgacagcaa agtagtggtc gctttgcatg aatatcgtcc
    33121 acgcagcaag tccacacaat atgcttcgag attgaaaagc cagcattcca ttgaaattct
    33181 gaaaacacgc gaagatatct atcaaagtga cacaggagag cttgtcgttc gggaaggtca
    33241 caaatttggc ggtgcgcctt actgtatcca ggaaccaaaa cttatcggct gggaggaatt
    33301 acaggcacag gggtacaggc aaatcctgca gatagatttt ccggggttgg aggatggcgc
    33361 cgtttcaggt acctggccct ttggcgatgg cattttcaac ttattttgga aatatccatt
    33421 cgaaggcgag aagtattttt ggtgcgtaca aagctgagac tcaatcttca atccttttat
    33481 tgcatcatat ccagatagga tttacatccg tcagcaaaaa ttcaaaggag ttttctgcct
    33541 tttcttttta gacgcgatgc tcaaaccaaa aaaaccttca aatcacaatt ccaagctgaa
    33601 aggatgcaca aaaaaacatg tccactgacc caacattccg catgaccata caggatgttt
    33661 tttccatcaa aggacgaggg accgttctca caggccgaat cgagagcgga acaatcaccg
    33721 tcggagatga aatcatcatc aaaggcagga attcaagcaa aaaagcgatc gtcgctggtg
    33781 ttgaagtgcg ccgcaaggtc acaaccagcg cccggtctgg cgatgaggca ggcattcgct
    33841 tgaaagacac cgaagaagac gttcaacaag gtgacatcct gactggctcc gagcatgatt
    33901 tcacctggaa gccgtagagc ggtttcaggt taatggtaaa cgttacacca ctacaaataa
    33961 aaattctggg attacggtac ggttttctct ccttttggcc atgcctgccg tccccgccct
    34021 ccgtcaaacc tccaatggta taatcccgac gaaaatatat aattccccac aaaagtggtt
    34081 gaaaaattga aggagaacgt catgtcaggt cattcaaaat ggtcaacaat taaacgcaaa
    34141 aagggcgttg ccgatgcaaa acgcggcgcc gtatttacac ggctggcccg tgaggtcacc
    34201 atcgccgcac gtgaaggcgg cgaccccgat accaacttcc gcctgagatt ggcagtggat
    34261 aaggcccgcg ccgagaacat gcccaaggat aatatcgaac gcgccatcaa aaaaggcaca
    34321 ggcgaagaca aggaaggcac aaccttcgaa gaactcaccc ttgaaggcta cggcccgcac
    34381 ggctccgccc tcatggtcac ctgcgtcacc gacaaccgca atcgcaccgt ccccgatatg
    34441 cgccacgcct tcagcaaatc aggcggtaac atggccgaac ctggcgcagt cgcctggcag
    34501 tttgaccgca aatcctattt tgccttccct gccagcaaac tctccttcga caaagccttc
    34561 gagctcggca tcgaagcagg cgctgacgat gtcgtggatg gtggcgacac catcgaaatc
    34621 tatgcccccg tcgaatcatt caagaccatc gccgatgccc tccacaaggt tcatgttcag
    34681 cccgaagagg ccagcctgcg catgatcccg aaacaggaaa tcgaactcga tgtggaatcg
    34741 accctcaaat tcatgaaaat ggtcgaaacc atcgaagacc tcgatgacgt gcaggatgtc
    34801 ttccacaacg tgaaggtttc agacgaagcc ctcgccgccc tcgaagctgc ataatcaaaa
    34861 tcatggtagg gacacagcgg cgctgtgtcc ctatattatt taatgaccct cgccctcgga
    34921 attgaccccg gcaccgccac aacaggatac ggactcgtgc gcctcatgcc caacggggaa
    34981 ctggtcacgg tttcctacgg cattatctcc accccaaaag acgccacccc gcccgcacgc
    35041 ctcgaaatgc tttttgatga cctcaacaag ctgttgaaaa aacacaaacc cgaaaccgcc
    35101 gcagtcgaaa aattattctt ctccaaaaat gtcaccaccg ccctggccgt agggcaggct
    35161 cgtggcgtga tcatgctctg cttgcaaaaa gcaggcatcg aaccttttga atacaccccc
    35221 aacgaagtga aacaagccgt ggcaggctac ggcagcgcag acaaacgcca ggttcaggaa
    35281 atggtccgcg ccctgctgca actggattcc atccccaaac ccgatgacgc cgccgatgca
    35341 ttggcaattg cgatcactca tttaaataca aaacgatacg agtagggaca cggcgctgcc
    35401 gtgtcccttt ttcaaatggc ataacggcgt tgtgccccta cccacacaat acggtaaaat
    35461 acaaatatga tagcaaccct gcgtggagaa atcacccaaa tcgaagagaa cgccatcgtg
    35521 gtcgaagtcg gtggtgtggg catgcgcgtc tttgtccccg ccccattgcg gaccaatgcc
    35581 aaggctggcg aaattctttt tatgttcacc catctcgtcg tccgcgaaga tgcgctgacc
    35641 ttgtacggat tcgaatcaca agccgaccgc gacctgttca acatcctcct cggtgtggat
    35701 ggggttggcc ccaaagtggc gttatccgtc ctctccaccc tcaccattga cgcgatccaa
    35761 cgcgccatct tcgccgatga agccgacctg ctcaacagcg tacccggcgt tggcaaaaag
    35821 accgcccaaa aaatggcgct gcatctcaaa gacaaactca aacccaccga cgcacttggc
    35881 aaaatcgccg ccatggccga ttacgacagc gaagttctcg ccgcgctcac cgcactgggt
    35941 tactccgtcg tcgaggccca ggctgcgata caatctctgc caaaggatgc gcccaaagaa
    36001 gtggaagaac gcttacgcac cgcgctggga tattttcaat aaaaaacgcc aagcctccga
    36061 gataaaactc ggaggcactt ttataaacac aagagtaaaa taaccaacac caattttcaa
    36121 cttctccctt tccctttctt catcattcat acttcatcat tcatcctttg accttaggag
    36181 ccttctcatg ctaaaaaaga aaatcgcctt cattggcccc ggtgtcatgg ccgaagccat
    36241 gatcgcaggc ctgctgcgaa aaaaactagc cgatgcaaag aacatcaccg cctccggtcc
    36301 gcgtgaagaa cggggcatgg aattgaacaa gaagtacgga atcaaagcca ccaccgataa
    36361 cgccgccgcc atccacgaag cggatgtggt cgtactctcg gtcaagccgc aacgactcac
    36421 cgaagtgatg aagggtctca aaggcatccg ctccgatgcg ttggtcgtgt ccattattgc
    36481 gggcgcaacc atgaaaaaga tcagcagcgg actcaaacac aagaccattg tgcgctccat
    36541 gccaaatacg ccggggcaga tcggagaagg tatcaccgta tggaccgcat ccaaagaagt
    36601 gaccgacgaa cagcaggaaa tgacccgagc catccttggc gcactgggcg aagaagtctt
    36661 cgtcgaagat gaatcctatt tggatatggc caccgcgctt tcaggttctg gccctgccta
    36721 cgtcttcctt ttcaccgaag cattgatcga tgcaggcgtt cacatgggct ttccgcgccg
    36781 cattgccgaa cagcttgttt tgcaaaccat caaaggttcg gcatcgtatt atcaaggcgc
    36841 aagcaggcac cctgccacac tgcgcaatca agtcacatcc ccgggcggca cttcggcaga
    36901 agccctgtat tatctggaaa aggcgggctt ccgcacggcg atctcacgcg cagtatgggc
    36961 agcctatcaa cgttcgctcg aactcggcaa ggaaaaacca ggtcacattg atacgcacga
    37021 cgaagaaagc gcgtaatctt tatcaaccgc aaaggacgta aagatttaaa atattttctt
    37081 cgcgatcctg gcggttcaaa atgaaaaaga caatcaccct cttaaccagc ggcacacgcg
    37141 gcgatgtgct gccctacatt gcgcttggcg aaggcctgct cgatgcaggt tatcaaatcc
    37201 gcattgctgc tccgcttggt tttgcgaatc tcgttcaaca aagcaaactc acatttgcgc
    37261 ccttcgaagg caatccttcc gacctgctca tcgagcaggg tgactccacg ccgatgacgc
    37321 tcggcacaaa tcccatccgc tccatccgtg ctacgcaaaa attcatcggg caggctcgtc
    37381 cgctgtatcg gcgcatgctc cataccgcag ccgaagcctg ccgcggttcc gatatgctta
    37441 ttcatggcct gccgaccatt tggggcgcgc atattgccga agggcttggc atccccgccg
    37501 tccgcgctgt gttgcagccg ctcgcgccca cccgcgaatt tccctccgcg cttttgccgt
    37561 ttcgttcttc ttttttgggg atcggaaact ggctcaccca ttgggcagtg actcaagcca
    37621 catggctttc gtggcgccgc gagatcaacc acgcgcgtca caccgacctt gggctgggtg
    37681 ccgctcactg gcttgatcct tcgcttcagg ctgattcaaa ccgacccctc accctgaacg
    37741 gattcagcac acagatcgtt ccacgcccga aagattggaa tgacaagcag gtggttacag
    37801 ggtattggag aaaaaacgag ttgaccaata atcacgttga caggccagac cttcaacatt
    37861 ttattgattc ttcccccaac cccatcgcca ttgggtttgg ctctccgggc acacgcggat
    37921 atttaccgat gctcgaagaa gctttgcagc atgccaatgc gcaggcgatt ctgaccatcc
    37981 ctgccaaatg gcatggcgaa atcaaatcaa aaaatatttt tcctatcgaa tatgcaccac
    38041 acgactggtt atatcgccgc gtgaaggtcg ccgtccatca cggcggggca gggacaactt
    38101 ctgcgagttt acatgcgggc attccaacgg tcacactgcc gttggcgatc gaccaattct
    38161 tttgggggga acagatccat aaaatcggca ttgggccgag gccgatcccc caaagaaaat
    38221 tgaacgcgga aaatctttcg caagccatca cacaggcatt gacagatcat gccatgaccg
    38281 caaaagcaaa aactgtcagc gccgagttga acaaagaaaa tgggattcag gccgcggtga
    38341 gtcagatacg gaaattacta tgacgtcgcc tcaatgggat taatccaaaa ctgtctggtg
    38401 agatgcattt ccccgcaatg acatatacgc cacccccaaa gcggtcaaag aaaaaatact
    38461 ccccacccaa tacggcgcag aaaaagcaat gacctcaaac gaaatacccg caagcatggg
    38521 accgaaaata tttgtaagtg aaagcaaggt ttgcgtaccg cccatcaaac gtccctgttc
    38581 gctttcagag acgcgcaacg aaaccaatgc cgtgagggat ggaatgctcg tgcccgtgcc
    38641 cagtgcaacg acggtcacag cggggtaaag catccatgcc tgcggggcga gcgccatacc
    38701 tgcaagaccg atgaccatga gcgaaaggcc aaagaggata aggcgttttt caccgaaacg
    38761 cggctggagt ttgccgaaca aaaatccctg aatgaaaaca gcgcacacgc cgacatacag
    38821 gtaaaaaaag ctgttctgtg tgggtgtcca atgaaaacga aaattggaat acagcggaaa
    38881 gttggattgc agcccggcaa aggcaaggtt gagcagaaac atcgtcacaa gaagtttttg
    38941 aatgttggcc tgattgaaca aactgcgaaa atgcttcatc cagttgaaca tttctgaaac
    39001 cgatctggct tcgcgttttt ccagcggaag cgattcgggc agcacgaaat aaccaaagat
    39061 gacattgccc agcgcgatcg cagatgcagt gaatgcggga acgtacaagc cgtatttgct
    39121 cagcaatcca ccaagcgcgg gtcctgccat catccccaac ccaaacgccg caccgaccat
    39181 gcccatgccg cgcgcgcggt tttcggaggt ggtcacatcg gcgatgtagg cataggcagt
    39241 ggtgaggttt gcgcctgtca atccgtcaat gaaaacggcg agaaaaatca gcggaagtgt
    39301 atcggccagg ccaagcataa aataccccag cgaagtgccg aacaggcaaa ccaatagaac
    39361 aggtctgcgc ccaaaccgat cggagagcgc acccaggatg ggcccagcaa acaattgcgt
    39421 acctgcatag agcgaactca acgcacctgc tattgcagcg cctccatctt gagcctgcac
    39481 gtaaaaaggc agcaacggca gcatgatgcc atagccgagc aagtccacga aaatggtgat
    39541 gaagataaaa acgagggagg aattcaaatt gttatcaacc gataaaaagt agggaaaagt
    39601 ggaaagtggt gagtggaaag taatcacttt ctactttcca ctttccatct cctaaaatct
    39661 ctccaaccgc agcggactca gatcgaacgc cgtctctttc ccgttgacca gttccgccac
    39721 cacctgccct gttccaggtc caagggacag accgagcatg gcatggcccg tggcaagcac
    39781 aaggttggaa tgattcggag agcggccaat gatcggcacg ccatcgggcg tcgtcgggcg
    39841 caaccctgcc caggtttcct tgatgtcgag cgtttcatca atacgaagat attcgcgtgc
    39901 agaattctcg atccgttgga tccagcgcgg gtcgggcgtc atactgccat tgccaacttc
    39961 aagtcggcct gtaaaacgca gcaggtcacc cattggcgaa accgccacac ggcgctcgcc
    40021 aagaatcaat gcatgacgcg gcatctgctt cgttgcagac atggtcaggc tgtatccacg
    40081 cgcaggctgg atgggaagat tcaatttcag atcgcgcgcc atggatggag tcaacgcccc
    40141 tgcggcaaga atgacatgct ccgcctcgtg cttcgcatca ccgacagctg ttcttacaac
    40201 ccgaactttt ccctgcgccg attcaagccc agtgacaggc gtattctcaa gcatctccgc
    40261 gcccatatcg cggacacggt cgctgagcat tttgagaaat aacgcggggt tcatatgtga
    40321 atcgcctgta aaatgcacgc cgccgatgac atcatccaat gcggcaggct caacttcatg
    40381 gaccttcgct ttgtcataca cgctgacggg gatgccatgc ttctgcatca aatcgccttc
    40441 gtgtcttccg ccgctgaagg cttcttccgt tttatacaaa aacagcagac ccttctcctg
    40501 ataatgacag tcgaattttt cctcggcgat catttgggca aagttcttcg cactcaattg
    40561 acccaggctg ttcaatggcg gaattccgcg ctggacgttc gattcactgc aagccagcac
    40621 aaaccgcagc agccacatga gatattttgg atcgagactg atcttcatcc caaacgggct
    40681 gtgagcaggg tcaagcatcc acttcaacgc ggaagttacc acgcccggcg cggccagtgg
    40741 aatgatatga ctcggcacca catgccctgc attgcctgaa ccgctgcctt tgccgatctc
    40801 ttttgaatca agaatggtca cttttctgcc agatttcaaa aggtaatacg cgcagcttaa
    40861 cccaacgggt ccgccgccga tgatgagaac atcgtgctgt tgcacatctg attttgtggt
    40921 catttttgct ccattgagct cttttcactt catgccaaca tatttggggg aatatgatat
    40981 tttattcaac ccaaaatcgt cactgggatt tttttttcaa ccaaatcaat gcttcattgg
    41041 ctttggggtg tcccaacttc ctggcttgca ggaagcaccg ttctgcgtct ttcaaattcc
    41101 ccatactggc aaataccctg ccaagattga accatgcggc aacattattt ggttccgtct
    41161 ttattacacc ctccagtatt tcaaaggcct catgatattg tttggctttt gccagttcaa
    41221 tcgcctgttt gacctgttcc gcgccagaag cggaattaac aaattgaggc tggtcttcag
    41281 gatgggactg ggtgttttta cgtttacgag agcgagacag gaagaccgca atgataacca
    41341 gcaggatggc aagaagcagg caacatatcc cgccatagaa taaagaggat gcacttatcc
    41401 cagaagggag cggaaggcta ggttgagaat tctgctgaat accacttatt ggtgttcctt
    41461 caatcgaaaa gttaggagga acaggggagg ccaatggcgg ctcaagggtg ttctccgtca
    41521 aatcaatctt tttttgtgtc caatctacga taaagtttaa tatataagga tcatagctaa
    41581 tatcatgaaa agttgggagg tcacctactc taaagctctc acgaacagga taatcaaaaa
    41641 gcatcaattc aacagaaacc tgttgtgcgg tttgagtata ctttttgtac aagaactggt
    41701 cggaatcacc ggacaaccaa aataaggctt ccgcatcatc tgcgaccaga gttggcgaaa
    41761 atcctttctc caccacaagc aagctctgat ctcctgccac gccatcgaag aaactgccgc
    41821 ctgccaaata ctcatcaatc aaaataaccc tgccgcttcc cgaccaaaaa ccatagaggc
    41881 gggagtcggg gttgtccagt ctggaagtgt atacagtctc tacactttct gtatcaaggt
    41941 ttagttcgta aagatacaga tcgtcactca acagaagccg tgacccctcc tggtttaaat
    42001 catacacttc cacactagtc agattataag tttcgcctgt cggaaaggtg tgctcacgag
    42061 ggggcaggat atcaaatatt tcgatctttt tgtataccga ccccgaatca aggttgatca
    42121 gggaatatac cttctcatct ccggtatgaa cgtaaaggcg tttatcgggc gaccaaaatc
    42181 cattagcact agcgctcaaa ccattaatct gcaaattcgt agtctggtta ttggcccagt
    42241 cataaaaagt gggtatctgt gaatttcctt ctgtgtagat gacaatgcca agatcaaagt
    42301 tgattacata tccattgtcg ccttcaacaa gttgaaacaa gccctcacgc tctccggtaa
    42361 taaattggcg ataatagttt ctttgatagt cgttggatgg atcaatctca gaatcgatga
    42421 aagaatcgct gtctttatgc cacaaaacag gcagtcccac ctcttcaaac gaaaacttcc
    42481 tgacaatctc ccccttcaaa acatcataga cgaaaacacc atctccaata cccgtatatc
    42541 catctcgatc aaacgccaaa taggccccat cgggcgaaaa actcaggtaa tcataggtta
    42601 cttccacacc cgtctcggga tccattgttc cagcgtcggc ggtgatacgc acctgccttc
    42661 catctccggt ggcaagcacg atatttccat ccgtgctgat aaaggcaatc gtcccttcaa
    42721 actgaagacc ctcctgggcc ttgacgggcg aaacagtctg taaagcgaac ataacaagga
    42781 ttatgaactt tcgatagtgt ttcatggctt tgttcccgtg agaataatga ggggtgtcta
    42841 ttttatctcc agtcagtata gaagcatcct acgaataata ccaactgaag atcttgttat
    42901 acaccgcgag caaaatcttc ttcaccaaac caacctccag cacacgccgc accaatcctt
    42961 ctttcttcct ttctctttct tttcccaact cccctctcaa ataccccagc gtattggaca
    43021 tattcatcgc cagcggatca ctgaccatca agcgcagcag acccgcgtca ttcatacgct
    43081 tatccaactg gcgcacctgt cccattggtt tgtccatgtc gaagggtaaa aacttttgca
    43141 gtgtggattt atacgccgtg aactgccaat gacttgcacc tgcaagcgca gtcatccctt
    43201 tgtattgaat tcgccaatcc tttgtctctg cgtagacctt ttcattctcc tccacagttt
    43261 gtccaagcga aagattgaac tctaaaaatg tttcccacgg aatgaattgc ccctcatcca
    43321 gggatgcatg ttctgaattc ctcgcccact ccagcgtgga agaatagaac tcaggcgaag
    43381 tgcggtatgg gcgcgccgtt accatgccga catttggaaa ggcttcgagc aattccaccg
    43441 aacgcgagag ccatgtcggc gaaaacaaaa cgtcactatc ggtataggca atgatctcac
    43501 cgggcgcgcc tgccagcatt acattccatg cgccgccctt gcccatgttc ttctcggaga
    43561 ggatcaaata ttgaattcgt ccctcctcct tctcctggat caaaaactcg cgcacctctt
    43621 cacaggaacc gttatcgaag accatcaggt caaacggcaa ccctgcatcc ttgcgcatgg
    43681 attcgagtga gaccttcagc acatccaaag tctcggcgta aaatccgctc aggaagggaa
    43741 tgtaatttaa caacgccacc gtgatccgtt cggggcgggc aacatctttg acgaacttgg
    43801 cagggttttg accttttcgc atgttaggtt ccaggttgga aagttggaac gttttaacgt
    43861 ttgaacgtta aaacgttaaa taatttctta aatcgtttct gccagcgctt tgggcgggca
    43921 atgttttcga tcatggcttc ggggagaaaa aagaaaatag aacgcaagac cagttcaagg
    43981 cggcgtgtga agcgaagcga tgtcatcttt gggcgggtaa gtttaccatc tacaagttga
    44041 tgggtcgcat ccaagatcgt gtatcggata ttcgcctgtc cgcctgccag acggatattc
    44101 tcattcgtat cgggatgatc ataatgcgac ctgcccccgg gcaaatggct gtaatcgtga
    44161 ttttgatgca cgatcatcac cgaaggtgtg caatcgatca cgggccagtt ttcgcggcgg
    44221 gctttataga tcatccagtt atcccaacct gcacgaccga tgatgaaatt tggaatatcg
    44281 gtatagcagg attttggaaa aaggaaaaaa tcgcttccag cgggacggtg tagctgattc
    44341 tgttttctga cagattctct cagcccagac tcccaccccg cagaaaaatc gagcagcgac
    44401 gtgatgtcgt aatcccaacg ttgactcaac agcacaaact tctcttccaa ctttgccgtt
    44461 tgttttgcgt tctcaacaaa atcaggcatg agaaccatat cggcattgat gatgcaaagc
    44521 aaatcaccgt ttgaattctc ccgcgccagt tgaaacatgg atgagatcaa cggtgtgccg
    44581 tttgcattgc gggcaacagc aggcaaatgc ttcacgccca gttcttttgc aatttcagca
    44641 aggcccgctt cctcaccaag caaaatgacc tccacatcag ggagcagagt ccacgatgtg
    44701 atggcgttgc gttggatcat cgcgatatgc ggattggtga aaggctttgg agcggaaaat
    44761 aaagtaatta gaggcatggg ggaggtttac aggtttgaag gttgaaactt gcgagctttc
    44821 caaacttcaa actttctaac gcttgagtgc gcgtttatca acatctttca aagtcttatg
    44881 ctgcacaccc tcgttgatct tcatcaattc gggattgtcg tgaacaagat ccaaaacttc
    44941 cttccaggta aagtcatcgc gtccatcaaa acgggcatag acctggcgca tgaactcaag
    45001 gtcttcgggt gtatccactg tccagcggta atcgccaaaa tcggtggtgt ggtgaagaag
    45061 agctatgtta aatccgcgtg gggaaacgcc tgtttgcagt tggcggttaa gattcgtcag
    45121 ttgcacacct tcatagaaat aaggcatggc atgttcacgg tgttggggct ctttggcttc
    45181 cttccaggct tttttcaaaa ccttgaaagt gcaggcctcc acatccaacc caatgggata
    45241 ggtgcgagtc cacggcggcg gcagacggtt gcaggcaaaa tcatattcgt cttcgagcaa
    45301 agtattgacc acgtgatcga tcagctctgg gtcaatgacg gggcaatctg ccgtgatgcg
    45361 gacaaccaca tcggctttgg cgtctttggc ggcctgataa tatctgtcca gcacatcgaa
    45421 caaactaccg cgggtgaagg gaatgccgct aaattcgcag tactcagcca cggggtcgtc
    45481 tgaagcgtcc gtcgtagtgg caaagatgac ttcgtcgaga gtcgtcgctc gagaggtacg
    45541 ggtgaagaca cgggtcagca tcggctggcc ttcaatgttg gcaaggatct tccccgtcag
    45601 tcgagaagac gacatgcggc cttgaatgat ggcaactact tttggtttca taattttttg
    45661 taggggcaca gcggcgctgt gctcctacgc actttgcgcc tcggatggaa taggcaacgc
    45721 ttcaacgaat cccttggcgc ggaatttttc gatgtaatcg cgcaccatcc agtaatcgag
    45781 cccgacctgt tcagcgatct caaaaaccga caactcccct tcaaagcgca tcatgatctt
    45841 ttcgatggcg cggttgagtg cccagttctc acgccaatcg acccagagac cattgccgct
    45901 gaggaacaca ggtccgcgga aggtgcgttt gggaatgtag ttcgtcgcat acatgcggat
    45961 gatctgctca gcggtttcgg ccgcaccgac cagcatgtca tcatgcatga tatcgaggtt
    46021 gtcatcggtg gtgtgatact cgtcataggg ccagcggttg atcgaaatgc aaggcacgtt
    46081 cacgccaggc ccgttgatca cgcgctcgtc attggcgggg gcggcagcaa aatcacgttc
    46141 atcatgtgct gacttgctga caataaaatg cgtgatgcgg tcaagcagat gcgtatgttg
    46201 acgggtatga tgccaggcga tcttgttctt gttccctgtc atctccataa agatgccacc
    46261 gcgcagattt tgaatcagat gctcgttgtg cgagagataa acgatggtgc caatggtctc
    46321 aggtccaaac cagaaacgga cgctcattga gcccgcaggc agcggctttt cagcgagacg
    46381 gcgcgccaat tcaatggtgc tgaccacacc tgcaccatcg tcattggctt gatgcgggtg
    46441 acaggtatgc gcctgcacga tgatctcgcc agctgaagcg ctcggtccgc cttcagggtg
    46501 aatgacagcg gttgccattt tgaatccctg tttcgggtcg gtgatgaatt cagatttgat
    46561 cactgcgcga tattttttgt cacgcggcag tttatcgaag acattcttgg gcaggcagaa
    46621 accccaatcg cggtcataat acttgaacac ccacggaatg gcatgggggc gtttttcgtt
    46681 gaaatacaaa tgcggctgaa gctcttcgaa cgaaagtgtt ttgtccacgg gcagggagta
    46741 cgaaacgata tgcaatggat tgttcttgaa atccaccaca cgctcgccgc cctcgatctc
    46801 aagatacgct tcatggacgg cgtaacgctc aggcacatac catgtccatg ctttttcaag
    46861 cggagtgtag gtctcgatgg tgtaattgga cgaatcgggc atgtaactgc cgatgatctc
    46921 caatgtcttg tcatggtcat cagaagcaag ggtgcggtgc agggggaaga actccgcgag
    46981 gatggatttg agggatgtgt agggtttcat
//
LOCUS       DAOQJC010000011        46880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_320_length_46880_cov_9.433294, whole genome shotgun sequence.
ACCESSION   DAOQJC010000011 DAOQJC010000000
VERSION     DAOQJC010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 46880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46880
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_320_length_46880_cov_9.433294"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /gene="guaB"
                     /locus_tag="PLE39_02775"
     CDS             <1..1277
                     /gene="guaB"
                     /locus_tag="PLE39_02775"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNE67275.1"
                     /translation="SEMAIAMAREGGIGIIHRFLSIDEQARQIQRVKKAESFIVDNPI
                     SMSTAHTVGDIKKLVDETGAGGILILDAEEKLVGIVTTRDLLFENDDKKSVTDLMQKK
                     VITASPDISLKEAEGLLHEHRIEKLPLVNADGRVAGLVTLKDIMKITQYPKATKDSRG
                     RLIVGAAVGVRDKEMRRVESALKAGADCIVVDIAHGDSNLEIDMIKNIRKHFPAAQII
                     GGNVATADGTKRLIDAGVDAVKVGVGPGSICITRMVAGSGVPQLTAVIECAEAARSAG
                     IPIIADGGIRQPGDVAKAIAAGAQTVMIGSMLAGTDESPGMVMTRRGHRYKASRGMAS
                     REANIDRNRKEGNDLTQEEIEEYVAEGVEAAVPYRGRAREVLTQLVGGLQSGMSYSGA
                     HNFEEFQKKAIFVRMTGAGLKESGPHDVEVLG"
     gene            1385..2722
                     /locus_tag="PLE39_02780"
     CDS             1385..2722
                     /locus_tag="PLE39_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNE67276.1"
                     /translation="MTNQETQNWIEKDKRQLHPTYHPKSHANPLVIERGDGVWLYTTD
                     GQKILDGMAGLWNVSAGYGREELAKAAYEQMKVMSFTSNFSGMTNLPSITLADKLAGF
                     AYEGLNTTFFTSGGSEANDSAFKTARYYWKRKGKPGKYKVIARKGSYHGITLAATFAT
                     GLEKYHTMFGPAMDGFVHIPAPNPYRYEGQLKAGETVGQAAARELEEAILREGEDTVA
                     AFIAEPVMGVGGVIVPPAEYFPLVRQICDKYGVLFIADEVITGFGRTGEWFALKHWNV
                     KPDILSFAKAVTSGYAQLGGIQISDEIRETMENTADTEAYMHGYTYSGHAMACAVGLK
                     NIEIMEREEFPKRARELGKRLYDGLQSLKEFSFVGDVRGLGLVCGVEIVSDKEARTGD
                     AAKTAQIFKAAQDRGLRTRPLGSTLAFAPPLSINEDEVDEIIKRLGAAMDASA"
     gene            2800..4782
                     /locus_tag="PLE39_02785"
     CDS             2800..4782
                     /locus_tag="PLE39_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HNE67277.1"
                     /translation="MSVYLHDIPLAQARARIREALQEANLWRVLGVEEIPLNENALGR
                     VTAEPIWAEISSPHYHASAMDGFAVRAISTNGAMPSSPVTLHVGPEAQYVDTGDPLPE
                     WTNAVIPIENVESLDENDQITKEIRKPASIRIRSAVAPWSHVRPLGEDIVATQLVLPA
                     GHALRPADLGAIAASGHQTIRVALKPKVAILPTGTELVPIGSKLKSGDILEYNSLVLA
                     AQIKQMGGEPTRYPITRDDFGLICERVFEASQSHDLVLLNAGSSAGAEDFSSKVVEKL
                     GTLLVHGVAVRPGHPVILGMVNRQSENLKPETLNLIPIIGVPGYPVSAALTVDIFVEA
                     LIAKWLGRPPMELPTETATLTRKLVSPAGDDDFVRVAVGQVGDKLLAAPLSRGAGVIT
                     SLVQADGLALIPSGVQGMEAGEPVKVHLYRSKAQIEKTIFCIGSHDMTLDLLAQFLAE
                     HDRRLASANVGSQGGLVALRRGEAHMAGSHLLDPSDGSYNIAYIRQYMPNIPVKVVSL
                     VGRDQGLIVKKGNPKGIKSLGDLTSHEVQFVNRQRGAGTRVLLDYHLNLMGMSSESIS
                     GYTQEEYTHLGVAAAVASGRADCGLGIAAAAQALELDFVPLFQERYDLVMPKVFAESE
                     LLAPLFDLLTDPVFRNSVSKLTGYDVNVMGRIIMED"
     gene            4787..5155
                     /locus_tag="PLE39_02790"
     CDS             4787..5155
                     /locus_tag="PLE39_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE67278.1"
                     /translation="MEDGLIIDDNRQTADALQQMLGVLDLPARVAYGSSPAISMLSSF
                     VPGFICLDINMPGVDGTEVLAFLRREPRLMKIPVIVITSDDQPETRQRVMRGGAQAVV
                     IKPATLDALENAFKRSGVLS"
     gene            5261..5620
                     /locus_tag="PLE39_02795"
     CDS             5261..5620
                     /locus_tag="PLE39_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67279.1"
                     /translation="MNQQEKTTMAPDEKTGGWKTGCLTGFLSGGVGVVGGVAVALISG
                     YFGFDCFADSQFFIEVFIFPVVGLLAGAITGAAAWLGITLSKGKASPLVVGGIIYFLF
                     FCILAVIVAAVAKAFSC"
     gene            complement(5617..6390)
                     /locus_tag="PLE39_02800"
     CDS             complement(5617..6390)
                     /locus_tag="PLE39_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67280.1"
                     /translation="MRKGLINYLICAVIVFGCSLPSQAPTATPLPPTATETPAFTNTP
                     EFTATPLATLTSTLTLTPTITFTPTITATPTFSFPSVTVNKQAHCRYGPSVAFLHAAD
                     LYPGDTGYVDGRYEYSSWLHVKFDKLKYYCWVAPSVVDVVGDISTLYYNEPDLQSVGY
                     SNYPPPQNIVAIRNGDEVTISWNQVEMTTDHDRGYLIIAFVCQNGAYLWWTFSYPDQF
                     TTSYTVQDEAGCAQPSEGVLYTVEKHGFSKPAQIPWPAP"
     gene            complement(6387..>6587)
                     /locus_tag="PLE39_02805"
     CDS             complement(6387..>6587)
                     /locus_tag="PLE39_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67281.1"
                     /translation="QHLVLSTGMDTFDGDPLGTFHVTRDGFSEIGKRIAALNLPTAII
                     MEGGYANEALGTNTVTLLEKFK"
     assembly_gap    6588..6687
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6688..7262)
                     /locus_tag="PLE39_02810"
     CDS             complement(<6688..7262)
                     /locus_tag="PLE39_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67282.1"
                     /translation="MKIFYSEEHRSHYPPFEVFDGGKRIPYYENPDRMDRILAALGKT
                     DWAELTEPQDFGLDPILAVHDGNYIQFLASAWTEWLASDPQVAASPEQNAFLPATFAL
                     RRKPRVPNSLLGKAGYYIMDLSACIVEATYKAALASANCALSAASSSLILQNASFALC
                     RPPGHHAGKDYAAGYCFINNAAVAANWLSQKG"
     gene            7309..7869
                     /locus_tag="PLE39_02815"
     CDS             7309..7869
                     /locus_tag="PLE39_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNE67283.1"
                     /translation="MELFEAIHGRLTIGKVKPEPLPRDVIEKLLSAGAQAPNHHKVRP
                     WRFVVLTGDGRKKLGDVMAASFLDRNPATPPEGLNKPRSLSLRAPLLIAVGADKPAAD
                     SKTIEVENLSAASAACQNILLAAHALGLGAMWRTGEWARDARVKEFLGFSTDQHIVGF
                     IYVGYPEVTPEPYTRIGFEDRTVWME"
     gene            complement(7931..8746)
                     /locus_tag="PLE39_02820"
     CDS             complement(7931..8746)
                     /locus_tag="PLE39_02820"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNE67284.1"
                     /translation="MTSYEDSIENHRIQRDEKTAANQLHWLNLAGLFWLEKGENSFGN
                     GTDNKICLPAFPSAHCGSFRLENGIVTLHPAKGADITINGKTPNASPLRTDREKDPDL
                     ISIGSLTLKIIIRGGEPLVRIWDRESPALKQFDGFHYYPPKPEYRVIAKYVRYDPPKP
                     IKITEVIGTEVESAFLGQARFTLNGVECVLEAEKSDDELLFNFTDGTNKDTTYGGGRK
                     FYLPIPKGDEIVLDFNLTENWPCAYTPYATCPIPPRENRLPIKVEAGELKYHD"
     gene            complement(8753..9631)
                     /locus_tag="PLE39_02825"
     CDS             complement(8753..9631)
                     /locus_tag="PLE39_02825"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNE67285.1"
                     /translation="MKLKTWQKILGILLIATILLRVTSRPAVDHPYYAADLNYPLVIA
                     HQGGDGLWPGDTMFAFQNAVDLGADVLEMDVHITKDGTLVLMHDETVDRTTNGTGEIE
                     SMTLDELKKLDAGYDWTPDEGATFPFRGQGVTIPTMEEVFAAFPDKHMTIEIKKTNAS
                     MAGPFCELIRKYNMEDKVLIASFHDDRLKEFRKECPEVATSSAKNETTVFVLLTKPFL
                     GSFYSPKFFSLQVPEESGGITVMTPAFVKAAHERNLAVEPWTINDEETMKKLIAWGVD
                     GIITDRPDIMIKVLGR"
     gene            9737..9961
                     /locus_tag="PLE39_02830"
     CDS             9737..9961
                     /locus_tag="PLE39_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67286.1"
                     /translation="MAENKFLEADMLALQLLSEGGHGPDTSLSWLLYIGIAFFVMIIV
                     FGWPEGSKKQGQAEGPREAPKGKKKADDLD"
     gene            complement(10070..10567)
                     /locus_tag="PLE39_02835"
     CDS             complement(10070..10567)
                     /locus_tag="PLE39_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67287.1"
                     /translation="MNALTLVLRLIHIVAGVFWVGAAWMLTLYIVPSATAVGDAGQQF
                     LRQMMGKTNFQKIMTTSSLSTVIAGTILYVRNPPTWFSSNAGIGFGIGAFFALIGLVF
                     GFLIGKTNMAMMQIGSQIKGQPTPDQIAQIQDLQKRQRTFSTINIYALLAAVVFMAIS
                     RYLFI"
     gene            10738..11136
                     /locus_tag="PLE39_02840"
     CDS             10738..11136
                     /locus_tag="PLE39_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNE67288.1"
                     /translation="MKKVLFAVLVLAALALAACGGGSNAVATLAPVPAEYAGQANPLA
                     ADAAAAGAEVFKTNCESCHGPQGHGDGPAGVALDPAPKNLPELAAQAGDDYLYWRINT
                     GKEGTSMVAWKGVLTDEQIWQAIAFIRTLK"
     gene            11240..11704
                     /gene="bcp"
                     /locus_tag="PLE39_02845"
     CDS             11240..11704
                     /gene="bcp"
                     /locus_tag="PLE39_02845"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNE67289.1"
                     /translation="MPIPSGIPAPEFEMLDDTNALRKLSEFRGKNVVLYFYPKDDTPG
                     CTKEACNFRDDYSAYEKAGVVILGVSPDDVASHVKFKKKFQLQFPLLADDGHKVCDAY
                     GVWGPKKFMGKAYEGVLRTTFLIDGEGMIKKVYENVRPAEHSAELLKEFGVS"
     gene            11880..12896
                     /gene="coxB"
                     /locus_tag="PLE39_02850"
     CDS             11880..12896
                     /gene="coxB"
                     /locus_tag="PLE39_02850"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNE67290.1"
                     /translation="MRHFVIVGILVIAMAVLTYVGLDAAGFATQMNPVAASAQAQTID
                     NMWHWEIVAMSFLFALIVAPMAYSLVVFRKRKGDDTDAQHVEGNTALEIGWTVGPLIL
                     VVIFAYMGAYSLGEVRRVDPEAVVIKVKAQQFAWTFEYPEYGVVSNELHLPANKQVLL
                     KMESADVIHSFWVPEFRVKQDVVPGRVTEYRITPTLIGVYKVRCAELCGTSHAYMEGM
                     IVVSEEANYEAWIAEQIAIAAAAQTPEGKGQLLTVSKGCVGCHSIDGTAMTGPTWFGL
                     FGADVKLADGSTVTADEAFISESILDPNATIVEGFPSPSVMPPFALTEEEIANIIAYL
                     KTLK"
     gene            12917..14812
                     /locus_tag="PLE39_02855"
     CDS             12917..14812
                     /locus_tag="PLE39_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNE67291.1"
                     /translation="MKRFISSALAHGLLGQLIGTVVGGGLVTGIRALMGLSVTDRFFF
                     TEPAWVLGGFIGAIAFLFSSGVTSDWVKWARGIDTPEHHEDHHSGWEKFVNVSLDHKV
                     IGIQYTIVALALISIGGLFALIFRTELAASELQFLTTDMQLFGQNGPQLYNTLMSLHG
                     MIMIVSILLGIAGIINYVVPLLLGAQDMAFPRLNAFSFWIAVPAAVLLLMSLFLGGFD
                     TGWTGYPPLSARAPVGMQMFFLGVFVAGWSSILGALNVIVTVLRMRAKGMSAMRMPIF
                     VWAAIATSLIALTATQFIGLSFQLVMFQRLFGMGFFDPAKGGNPVLFQHLFWFYSHPA
                     VYVFILPGLGVISELLPVFVRKPLFGYRWIAMSSLGIALVGFVVWAHHMFTSGMNEYL
                     RVPFMYSTLLVAIPTGVKFFSWVATLWGGKISTPTPMLFVLGGIVVFLMGGLSGPPNA
                     IVSTDLHLHDTYFIVGHFHDTIFGGFVFPFFAALYYWFPKATGRRMNETLGKVHFWLM
                     TPSFFVLTFGMMYVGLLGMRRRIVDYDPALGLDATHLVLTIAGYLIALSVVIFIYNFF
                     SSIKNGEKAEGNVWNSRSPEWQVPSPMPVHNYDQPFEVISEPYDYGLEGGHAYIKFSA
                     PESGAHK"
     gene            14827..15612
                     /locus_tag="PLE39_02860"
     CDS             14827..15612
                     /locus_tag="PLE39_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNE67292.1"
                     /translation="MSHTQTKSAAYGQGVVIFIYLAILTALEYFVAITFNAVSILVVV
                     AVIKAALVMYYYMHIYKLNEDSEGDEHSYDYKTGTNRIGLWLFLLSDGFVFAGLLVTR
                     INLLGLTRPHLNQTLGLAVTAVLLVSSFFMNRGETAMAHGDKKGFMNNTLITFVLGLG
                     FLLGVVMVEWRLAAEEGLTASFGNPAVGPMGGVFYIMTGMHAFHVLTGLIFLLIVWNN
                     ARKDVYSAEKHWGVEAAAVYWHFVDLVWIFFYPALYLIGKLVN"
     gene            15715..16326
                     /locus_tag="PLE39_02865"
     CDS             15715..16326
                     /locus_tag="PLE39_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNE67293.1"
                     /translation="MDKRITFVGLASLGIVALAFVLNIFFLKPASFRGALYGEPYPRA
                     SEIELPMANGQTFRLTEQHGKVILLFFGYTSCPDVCPTTLAEMKMVMDQLGDDGSRVQ
                     VVFVSVDPERDTPEKIQTYVEHFNPAFIGLSGSSEQLEPVWQAYSIFREAVQSDSAFG
                     VIINHTARLFLVDSQGNLRLSYAYQTPVDDIVHDIKLLLEREP"
     gene            16323..16847
                     /locus_tag="PLE39_02870"
     CDS             16323..16847
                     /locus_tag="PLE39_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67294.1"
                     /translation="MNFSIKRIVISFLLGLVLAAITTEAAYFFLKKENRAPGLIELVI
                     PAGTADSIRAGNAPVEIPRDMRFVVGDTLRVINQDDENHQLGPLWIPAESSASLKLET
                     AENLIFECSFQADNNFGVTVMEPVTWRTRVSGIFFAGFPLGMMFAVYGGLIGEKKKKD
                     NEKKDDGSSQGNLA"
     gene            16816..17550
                     /locus_tag="PLE39_02875"
     CDS             16816..17550
                     /locus_tag="PLE39_02875"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HNE67295.1"
                     /translation="MMVLRKAISRKWFFASLLVLLGTAFCIRLGFWQLDRLEQRRAFN
                     AQVESMRAAEMLDLNQAIPQDIAAMEWRAVTVTGEYDFENQVAIRNQYNGDQYGYHLI
                     TPLLSNGTAVLVDRGWIPADGNATPADWRQYDEQGTLTVTGQIRLGHGKPAIGGIADA
                     LPAAGTRLEVWNNLDVENMSAQFPYPILGVYIQPNVEPVDEVPPIPYQPIIELTEGSH
                     FGYALQWFTFATILFVGYPFYLRKQE"
     gene            17556..18872
                     /locus_tag="PLE39_02880"
     CDS             17556..18872
                     /locus_tag="PLE39_02880"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNE67296.1"
                     /translation="MKYSLRSSFARLTLSLFIMVFLLTIVGRIVTVTGAWAYCLGWPL
                     CVPSEPIGYLKLAHLILVGLAMVLMFWLLRKAWREQRHQQVILPLTTVTGVLFFGQAF
                     IGAIEVTRSYPTHLVVLHALTAVALWISLGSLVYLSGALAVDSNEVVKFDYRQRAKDF
                     FILSKPIIVLLLLVTTYSGLVAGSKAWPSASLTFWTLLGGTLAAAGSSALNQYIDREL
                     DKNMQRTSKRPLADGRLTAAEGLSYGLALCLTSYYVMAGFVNLLAALLSLAGIFYYVF
                     FYSVWLKKATVQNIVIGGGAGAIPPMVGWAAATGQLGLAAWILFAIVFMWTPPHFWAL
                     AIVRMKDYERAGVPMMPVVHGEEKTRRHILVYTIELIAVTLLLPIFNLAGMVYAVSAI
                     VLGGFLLYAAWRVFKVGGNKVAWTMYRWSSMYLAFIFLALMIDAVV"
     gene            complement(18909..20408)
                     /locus_tag="PLE39_02885"
     CDS             complement(18909..20408)
                     /locus_tag="PLE39_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE67297.1"
                     /translation="MKSQKAKLILVLVIFLLTRLAGLVTRPIWYDEAFSILFSEKGLN
                     AMIYGTLSQSGSGAADIHPLGYYVFLWAWMRVFGTSILAARLLSIFISLAALVLVYLM
                     ARELFDETTAWISALLFSILPFQVHFAQEIRMYMLLSFWLLAATYAFLRGRGGEWKWW
                     LLFGVCSALAQYTHHLASVYLIPLALTPLWQKDWKTLKSVMAGALLAVLLYIPWLIHL
                     PAQLSKVQSSYWVERPGLEKIFTLILFYLPHLPLPNGLLLPGLLIAMLVIVLAVFQTI
                     LAKRNLIANTGKATWLVTLAFLPPLLLWVISQFVPVYIERALLPSHAIFCIWLAWAFT
                     QTRLPNPVRIFTFTLIGIAAVIGLSQHFFYQGFPYGPFREINQSIQSRLENGDAIIHS
                     SKLSYLPSFYFNRKLVQTFIIDPPQSNVDTLAPATREVLQIRSAEDIETASANATRIW
                     FIIYQQSVDEYTAQGYKTHPHLEGLEENYELEFIENWNDVRIYLYVKKP"
     gene            complement(20405..21934)
                     /locus_tag="PLE39_02890"
     CDS             complement(20405..21934)
                     /locus_tag="PLE39_02890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE67298.1"
                     /translation="MKQAQSHEKDVTGFFLLLAVVFGLAVRLYPILKTDFPLVDGGMF
                     YTMVNDLRAEGFSLPLVTSYNQADIPFAYPPLGFYLAGLINLLTGISVLNILRWLPFL
                     ISMLNIPLFYFMAERLIESKPKAALAALIFALTPSAYWWNIVGGGLTRSLGVFFFTAV
                     AFCVHQMFQRRSWSWVVAAVFSAAGCVLSHPAWAVQAAVASLVLWYFFGRDRQGVFYA
                     VIVAISVLLLTSPWWFNVIRHHGLETLLHAGQGSYSRLKIWTVFFTLSFTDEYTPVIA
                     VFALCGIFMHAAKKDYLPIAWAAMGLLAEPRGGLPASTLPFSIMAMTVLSDGIATRLT
                     PSTEWMDSLKQHSGRSFFGFFILLFTYNAFQVSNTLSYQVLTKEERTAIEWVRSNTDP
                     ADRFLVLDDEGNPLHSPLTEWFPALSERRSVATIQGTEWLSGKSSYANQYDVVKNIQQ
                     CLYADMNCIHNLENLLTDEYSYILISTAGGQSPLGSDLNAQANAALVYSSTSVKVYRV
                     K"
     gene            complement(21931..23385)
                     /locus_tag="PLE39_02895"
     CDS             complement(21931..23385)
                     /locus_tag="PLE39_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67299.1"
                     /translation="MTRTERFFITIVFLLVAAIAINPPVDNDMWWHLRAGDEMWHQGR
                     ILLTDQFSYTKQGADWVNAFWVSDLALYGLWQVGGFFAITLTTALLAIFTLWIIFKRM
                     TNAIIPRGLLVILAMMGIIANWTPRPQLLSFLLLAWLDSFLYEHIHTKRQPLWKLVLL
                     FALWGNLHGGFIWGALLLFATLAGESFDLLFGNSPKLVFKEIGHLALWSLAAFLAVAL
                     NPNGFALWKLPFQQVDVSLAIQEWLSPDFHQIYAHPMLWLMFLLVFGLGFSGKRLSFT
                     DLFKGLGFAYLFFFAQRNLVAYTIIILPIVEKFLAPAWANLTEAPQVKAFLERFSRQA
                     SKGQLSPGVAGVVNNAVIGMFIILLVGYAWLVSIPASIEKEVPAQAVAWVKEHQPSGP
                     IFNSYNWGGYLTWSLREYPVFIDGRADLYGNDLIQEWVEISNGTETGLERLESRGINL
                     IFLEPYHTLLEKLDPQTWVQVYSDSQIVIYQRRP"
     gene            complement(23382..24581)
                     /locus_tag="PLE39_02900"
     CDS             complement(23382..24581)
                     /locus_tag="PLE39_02900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNE67300.1"
                     /translation="MKINIDLSRTAITAILISLALVYPLLWLKMLADPTQHTGADFIA
                     FYAAGRIADQEGPSHAYDLILQKKYQEGVVQREIELQETFPYIHPPFVIPLAQIVSNA
                     DYLKSFQRWAMLMLLLFTAGIPFLIAPARNFFSQPQTLIFAAGIFLFFPIFQSLLLGQ
                     DNAIIFLGLSLLMWGTYAKKDWAAGLGLSLATVRPHFAFFLLLPFLFQRRAIFGWFLL
                     FVFALTLFSFGVVGTDGLKGFLHILTVSGSGEGYRTSEENMVNLIGLLRRLFPFLSAA
                     LIRSAGWAFYGLAFFSTGVYFWRNHTSPSGEEISLAVVAALFFSPHTHAQDLVLLIAP
                     IMAVILILLEKQRIQTAQAVLIPLAISLLLLFSYYSPILTHSIPYLIMAGLFFAVKQY
                     KALPAKP"
     gene            complement(24654..25556)
                     /locus_tag="PLE39_02905"
                     /pseudo
     CDS             complement(24654..25556)
                     /locus_tag="PLE39_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(25623..25868)
                     /locus_tag="PLE39_02910"
                     /pseudo
     CDS             complement(25623..25868)
                     /locus_tag="PLE39_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
     gene            complement(26039..26779)
                     /locus_tag="PLE39_02915"
     CDS             complement(26039..26779)
                     /locus_tag="PLE39_02915"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNE67301.1"
                     /translation="MPRKETKPTAKASPRRKKTNVVTESTFTRESTGTQIQKPVIGIG
                     TWIALVLFAGLVTFAIFLNKKEETAVEATPTSGTVPLFPATEGTPSSIEVKPAEGEAV
                     RVLRNEENVWVLELPFETEADPGLSEAAATQVSALQEISTVQGDLADFGLENPAFVIT
                     VGFASGKTHTLEIGDMTPSNRGYYVRVDEDRLVVVAVSGLDALLQLQFSPPYLNTLTP
                     TATTPPPTETPTPAPPTETPEASVTPTP"
     gene            complement(26783..28402)
                     /locus_tag="PLE39_02920"
     CDS             complement(26783..28402)
                     /locus_tag="PLE39_02920"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNE67302.1"
                     /translation="MIDNKNNSPAKYAFIGLIVAGLACVSSGLTGIAKGMVSQQIFTL
                     EETLTKNIDLAFNISLALIVIGLAAYAIMVPDQIRRFLTGRQARYGSNSLILIVAFLG
                     ILITANVLVFRNPDFLKSPWDMTEDKSNTLAPETLQALETLPGDVTATAYYSVNLDNA
                     SAEELLTKFKNNSNGKFTYKFIDPDQDPVSAREAGITGDGKILLQMGETKEIVSFASE
                     SELTQALIRLINPGERTVYFLQGHGEPSLEAGGGQLSFSIAKSTLESKNYTVSPLNLI
                     ATNKIPEDALAIIIAGPLKPISSGEMVLLKDYMEAGGSLVVMEDPTIVTEFGDTFDPL
                     AKYLDSDWGIKLNEDMVIDLVNTQNPLQAVSSQIVDHPITQNLTENYIVILPQARSIS
                     VSQEPIENVSTTPIILTTDQSWGETEMSAETQPEFSPEKDTPGPLNLAAVAENSATGG
                     RVVVFGNSVFATDEIFDAYGNGNMFINSVDWAAEQEDLINITPREQTQRMFTPPTNGV
                     FIIMIIISIFVLPGLIVFSGVSSWIARRKKG"
     gene            complement(28402..29142)
                     /locus_tag="PLE39_02925"
     CDS             complement(28402..29142)
                     /locus_tag="PLE39_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE67303.1"
                     /translation="MRNIWTIAKREYDNYFNSPLAYVVAFAIVLPIGIYFALIMFVSA
                     QQTMMGGPAPDSTPINWLFVFLLIFLSPALTMRLLSDEARMGTLELLLTAPVRDFELV
                     AGKFLGAFMFVLTIALFTLIFPIIIDNYVTPGVDWMIILSSYIGLILISAALLSLGTG
                     ISAIFTNQFAAFFVTFGLFFFLWFMVNLPASYLQQGGDVYNYLSLSQHFSSMNRGSIA
                     LGDIVYYISLIALGLFAGTTAIEIRRWR"
     gene            complement(29154..30083)
                     /locus_tag="PLE39_02930"
     CDS             complement(29154..30083)
                     /locus_tag="PLE39_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE67304.1"
                     /translation="MIKVSGLTKDYGARRAINNLKFDAQQGEIVGFLGPNGAGKTTTM
                     RILTGYMPPTDGEAIVAGYNVVEESLEVRKRVGYLPETVPLYTDMTAFDYLKFMGELR
                     KIPNIDDRVEEVLDMVGLIDRADSYIGNLSKGMRQRVGLAQALIHRPEVLILDEPTIG
                     LDPGQVVEVRELIREIGKERTVLLSTHLLHEAQNICDRVLIINKGKIVTEDTTQNLQS
                     RLIGGERVVVRVRGEADANLVNLIKAVKGVEHAVLTKDNSVEFDFVSGKDARPEVAKQ
                     VINSGYDLLELHPLGMSLEEIFLELTGSETKNN"
     gene            30269..30994
                     /locus_tag="PLE39_02935"
     CDS             30269..30994
                     /locus_tag="PLE39_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HNE67305.1"
                     /translation="MTELLYQTDAYLKEFSATILSVDSENRSVILDRSAFYPGGGGQP
                     CDFGTLEVAGVVYPVEKVKKQGDDVLHFLGGTEPLPTSNSASRCAGTLDWVRRHQLMR
                     THTALHILCGTVFRDYGAKVTGGDMDPLKGRMDFEFETMRGELVRDIEAAVNLEVQNR
                     RDIRVKILPREEAFQIPDLIRTKINLLPEGILQVRTVEIVGLDLQADGGTHVANTAEV
                     GRVKVVDYKSKGAINKRLYIEIE"
     gene            31067..31474
                     /locus_tag="PLE39_02940"
     CDS             31067..31474
                     /locus_tag="PLE39_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67306.1"
                     /translation="MKLKNLSLLLVVAVLFASVLACGSTAPVGVSNVYMANDEEGNNK
                     TTTFAPTDVIYVFFDVNGIDEGAQFQVKWYALTVDGQDPNEAFTTTDYTYTDEATIYA
                     NIESTDGGFPAAQYKVEIYLNGAKVGEQQFSVQ"
     gene            31649..32623
                     /locus_tag="PLE39_02945"
     CDS             31649..32623
                     /locus_tag="PLE39_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE67307.1"
                     /translation="MEYRKFGKTGLKVSPLCMGTMQFGWSLGEAESHKILSATFEAGI
                     NFFDTADIYSKWVDGNPGGISEAYIGTWMKQNKIPRDQIVIATKVRGEMGNGPNNEGL
                     SRVHIMNAVEGSLRRLQTDYIDVYQSHWTDESTPIEETMQAFDDLVRQGKVRYVGASN
                     YAAWELMQALWVSDKLNLARYDSLQPHYNLIYRDEFERELRDVCKTYEIAVIPYSPLA
                     GGFLTGKYRRNEPLPESKRAEGRKRAMTEKNFSLLYEMESIGLRHKASIAQVSLAWML
                     SDAVITSPIIGATTIEQLQENLGALNVHLMDDEVATLNKLTAWKTDED"
     gene            complement(32716..34275)
                     /locus_tag="PLE39_02950"
     CDS             complement(32716..34275)
                     /locus_tag="PLE39_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNE67308.1"
                     /translation="MSRKIIPFIIALVLSCLVMVVAGVFAFSGAVTAEKFGSDPVWSS
                     EYPTAESMKIIDLTGDGQDELFIQNAFGIRLYDPNGNVISDFTYDKPKTTLGDVNGDG
                     VEDIVVYHSGNFFSVDTIINGQQKTIASELPVFVPARVALIRFEGGSQIVLGDVSGQI
                     ASITTDGKIVWESDFASEEIRGMDDAKIGGQTYAAIASHDGTVGVFAANGSAVWQLEQ
                     EQLRRMRAFDLNADGNSEIITGGELGAFYIYSAVDGSTIFSKSLGQAVSEVREVELNG
                     DPSSREIIVGGKDGGIWAFSYNGTTATQMWTASLSDKVTEISGLDINEDGKEEAVIGD
                     DSGKVAVFTENGTRNNLPSHNTGITRIDIGKLGNERYVVIADGSHVQTNKVNFNSIPG
                     FQFTPLIVGLVVSAIMLVIAGVLASIPPKPELKVSFQDKSKESLEAQRRMLKESIADV
                     ERLRKAGEVTGDAYLARLKRLRSDLAENETAYKTAGYTIKVETFQCPHCGGTLELGMD
                     KCEYCGHVILS"
     gene            complement(34276..35220)
                     /locus_tag="PLE39_02955"
     CDS             complement(34276..35220)
                     /locus_tag="PLE39_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNE67309.1"
                     /translation="MPALCVLGVVGLVVVSLFYYLLNTARKKVKAPTNIAGKKEYAPV
                     YVSIADKPDSIMRGMERFKAEVQKTETAGDKWRWIPLMIFFVGLGMMAVDGVLLLFGY
                     FSLVFTSGGLLLWIAAFIMARSLRRSDTLDFPPRYKGTKDILYTLRDDLKPGSTFLGH
                     LDLTGAMLKNKVARETHDTQNRTTHHFRDEWLSLKAKLYDGNVLRISAIQKTKKRASY
                     WKRSRISGKSKLKPEKYKGTSHDLKVRIVVNPEAYQIVPNKEFRPGVNIGKYTISQLS
                     TDGGMINMVASSPFDEVDNEQILAILKSTYSLLQRKAA"
     gene            35294..36457
                     /locus_tag="PLE39_02960"
     CDS             35294..36457
                     /locus_tag="PLE39_02960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE67310.1"
                     /translation="MAKILYTAFDIVPSPKGASTHILHNLRGLVNGGHSAHLITPNDG
                     LLPTEDTIEGAQLTRISQDLSQNFLARAMHFGRSVAAHLALHQGYDVVHFRNIWDGLS
                     IAQNKKRLGYKTLFEVNGLPSIELKYHYPGLDPELLLKIKEQEIATLHLSDAIICPSN
                     VTRDYIASLGLDRKLVTVIPNGVSPSDFSPSSLPARDGRIPVLLYIGTLADWQGLDIV
                     VKALPKILEKQPVHLKIVGRGRSRQRKVLAKQIRKLGIEEHVTVQPAVPHHEIPALIA
                     SADICVAPLGLNDRNVTQGACPIKVLEYMAAERPLLASNMPIVRELVREDMDALLFSP
                     SDPEDLARQTITLLNDFELSKRLARSASERALTKFTWHESQKKLLKVYEKLLA"
     gene            complement(36492..37280)
                     /locus_tag="PLE39_02965"
     CDS             complement(36492..37280)
                     /locus_tag="PLE39_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE67311.1"
                     /translation="MNAYLAALQTESLKAFRSKVPLFTLLGFLIAPLIGGLFMVILKD
                     PEAAKSMGLIGTKAQLSAGVADWPTFFNLLAQATAIGGAIIFAIVTTWVFGREFSDHT
                     VKELLSLPTSREAIIAAKFTVIGFWTVALTLLSYGLGLIIGNAVAIPGWTNELFRSAT
                     IDILGAGILTILLLPFVAFIASIGRGFMAAFGWAIFTVAIAQVAVVMGWGDWLPWSVP
                     ALFSGAIGAHSETLGMHSYLIVAIASAVGFFITFYWWRNADQAK"
     gene            complement(37277..38185)
                     /locus_tag="PLE39_02970"
     CDS             complement(37277..38185)
                     /locus_tag="PLE39_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE67312.1"
                     /translation="MEYVIETNNLSKTYGSINAVNSVNLRVKQGEIYGFLGLNGAGKT
                     TTIRMLLGMIRPSEGNVKVLGQVLGPGRSGPWAQVGHLVESPSAYPDLTVRENLDIAR
                     RLHGLKNSNAVDDVMESLSIASYSKRKAGALSLGNLQRLGLARAMIHHPSLLILDEPA
                     NGLDPAGVVEIRELIANLAKNGVTVFMSSHILTEVDRLATRIGIIHKGKMIEDLDANK
                     LEQFRARRLKIKVRDFARAHAVLTRENYSATITEDSIFVSDTRALETPEKIACLLVNA
                     DTPPTHLAVEQQNLEEHFMQLTGSTK"
     gene            complement(38187..38786)
                     /locus_tag="PLE39_02975"
     CDS             complement(38187..38786)
                     /locus_tag="PLE39_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE67313.1"
                     /translation="MPKAFSEQEKDTIRLQMREKGKKLFEKHGLKKTSVDELTEAVGI
                     SKGAFYLFYESKEELFLEILEQFENEIQTQILEFTIAPKANAQKNVSEMLKGLLLTWD
                     AYPLLKNFSKTDFDYLVRKIPSERVMQHASNDEAFTTEFIKKIKREGINVKAPPRVVS
                     NLIKSLFFMGLHRDDMGETAYQETIGVLIDLVAGYIAGE"
     gene            complement(38932..39303)
                     /locus_tag="PLE39_02980"
     CDS             complement(38932..39303)
                     /locus_tag="PLE39_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE67314.1"
                     /translation="MTKILIVDDDKQATDLLQKVLTVEGYETTAVNDSSKAVQIADSV
                     NPHLIILDLMMPDPDGFKVCRMLRQTPRFIFTPIIIVTALDDSDSKVVAFGAGADDYL
                     TKPFHIDELSQRVKELLGRTE"
     gene            complement(39444..39803)
                     /locus_tag="PLE39_02985"
     CDS             complement(39444..39803)
                     /locus_tag="PLE39_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE67315.1"
                     /translation="MSKILIIDDDMETTKLLESLVKMNGHSPTSINNSADAIETANSI
                     IPDLILLDIMMPRINGIQLCKMFQSTQTLKHIPIIIVSALNDIGSKKDAFNAGAKDFI
                     TKPFLPKELAQKINALT"
     gene            complement(39939..40298)
                     /locus_tag="PLE39_02990"
     CDS             complement(39939..40298)
                     /locus_tag="PLE39_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE67316.1"
                     /translation="MPKIMIVDDDPQVITLLKRYLSVEGYEVIATTHSAKAVPLANTE
                     HPDLFILDLMMPPPDGFTLCSMLRADPAFRLTPILIITAMADSNSKATSFGANDYLAK
                     PFNLDELSLRISTLLNG"
     gene            complement(40446..41576)
                     /locus_tag="PLE39_02995"
     CDS             complement(40446..41576)
                     /locus_tag="PLE39_02995"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE67317.1"
                     /translation="MSNQNIDTIIIGAGPAGLAVGACLKQANHSAIILEQDNAVGPVW
                     RRHYDRLHLHTDKKNSELPFAPFPKEYPRYLSRDQLVDYLEAYAKKFDLNILFNQQVT
                     SARKINDRWEIQTNNAQYHASQLVIATGYAREPYIPDWNGKDGFKGKIIHSSEYKNGS
                     EFKGQRVLVIGFGNSGGEIAIDLYEHGARAGIAVRNAVNVIPKELAGIPILSIGIVQN
                     LMPVWLADAINAPILRAVIGDITQYGLRKLPYGPATQIRNDKHIPLIDIGTMKLIRAG
                     HIDVYSGVENFAGNRIKFTDGKEADFDSIILATGYQPRVNSFLQEQSVYDENGTPIST
                     GREAGVTGLYFCGYYVSPTGMLREIAMEARQISAAIARKRSL"
     gene            complement(41621..42064)
                     /locus_tag="PLE39_03000"
     CDS             complement(41621..42064)
                     /locus_tag="PLE39_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67318.1"
                     /translation="MPISYDISNTFNLLVYICTDSWTGTAFFKTADKVPEDPRYCSNM
                     KIIIDIFSAEIETSLSDMHLAIEKNRQLKQKGQKLGQTAVFTKSTSLMFLADAFKLMS
                     PEAPSNFGVFNNKQDVVRWLGFTDAEKEVIRFWEGAKTSPPEKVP"
     gene            complement(42198..42578)
                     /locus_tag="PLE39_03005"
     CDS             complement(42198..42578)
                     /locus_tag="PLE39_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67319.1"
                     /translation="MPWKISYLEDTKIIKTIYTEPITLDELKECAMANFSMAAEKQTG
                     LFLGDCSSFTQQGSTMDIYELGKFLESISLELRIHIKEALIEPSNIQRVSSDLRFFET
                     VTNNRMIKARIFQDTESALEWLLN"
     gene            complement(42636..43013)
                     /locus_tag="PLE39_03010"
     CDS             complement(42636..43013)
                     /locus_tag="PLE39_03010"
                     /inference="COORDINATES: protein motif:HMM:NF026926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm10 family immunity protein"
                     /protein_id="HNE67320.1"
                     /translation="MKFTAIYIEPYANTVTANVMFADPETDPDQPSVLSFSRPIEPGF
                     ENSSYYFEINDQSYGSYGGLETVQVSRNSLVVRVSPEKVQEFGEEDFREIQVDFQVDD
                     ETYGNVLAQLKRIFEGFDILQIQ"
     gene            complement(43091..44467)
                     /locus_tag="PLE39_03015"
     CDS             complement(43091..44467)
                     /locus_tag="PLE39_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNE67321.1"
                     /translation="MESRILQLIAALRASGVRVSLAESAEAFSAVDLMGIQEREAFRL
                     SLRATLIKDVKDIPTFDKLFPLFFGSGQPPMMGGNPTDDLSPEEIQMLAEALRQFKES
                     LRQNMERLMNGEPLTKSELEALGQLVGLNQAGDLNYQNWMAQRMMRAMAFPEIQKAMR
                     ELMEQLQEMGMNRERIEQIRNMVQQNMQGMQNQIEQFAGQRIAENLSEKPRGENIDNL
                     MNRPFQALSDADKKILQREVKRLAAALRTRIALRQKRMKSGQMDPKATIRANLKYHGV
                     PMEIKHRDKVRKPKIVVICDVSTSMRFCSELMLSFLFALQGQVRKTHAFAFIDHLESI
                     SEDFNGANADEAIQSVLWRMPSGHYNTDLGWALDNFNSEHMDTLNSGTTLIIVGDGRN
                     NYNDPRIDIFSTMTRRATRTIWLNPEPPAMWHGDSDMAKYAPLCSNVLKVGNLRELAQ
                     AVDELMTG"
     gene            complement(44499..45392)
                     /locus_tag="PLE39_03020"
     CDS             complement(44499..45392)
                     /locus_tag="PLE39_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNE67322.1"
                     /translation="MTTQETFTRPTTIAEMQERMKNFATPEGWKKGLAYKPAPTDVFI
                     VTPPKCGTTWMQQIVHGLRSRGSMDFDEITRVIPWINLAHDSGIDIDAPQVAHPRAFK
                     THSTMDEVPQGGKYIVVLREPRDALLSHYYFFEGFFFEKGSIDIETFAKEYYLPGRAV
                     FKHIAALWERRKDKDVLPLCFENMKADLPRTVERVAEFIGITLDDELKEIVVRQADIK
                     FMQAHEDKFEDHIIRKSRSAAMRLPMDGQLSKVRDGQVGGAKLSVPENIKQELDAIWE
                     SELMPKIGLNSYEELKKELMH"
     gene            complement(45429..46631)
                     /locus_tag="PLE39_03025"
     CDS             complement(45429..46631)
                     /locus_tag="PLE39_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67323.1"
                     /translation="MPPKQFILISLFAILTLSLGVPSAEPVTVPQNIPTATVTVPPSP
                     TPTRTALPTPIPYYITATVWESQPAAAILTYHKFKTNRPSTYSTVSRLDFEEELESLY
                     RSGYVSVSLEKWLAGDIRVPVGKRPVIFSMDDVFFRNQIALTAEGTPAKDTGLGVAWE
                     FYKKHPEFGFHWALFSNLGDKPYGEGTSIEQKIQLANVIVWCIEHDAMIYNHTFRHVN
                     LKNTKGLGVTAELRWNDETLRELLQLVHRADLIPKLRNMVALPGGRAPRWEDSQTALY
                     GYTDPDGRKVQAIFNIDYITRPDFLKPPYARKFNPANLPRIVANLEAIEYLVENKEKY
                     PAAKSCRLGPLDQGQSADSVYLAGQVSAAVRSGQCPHGVYVTDKFIFSAETEQTSLIQ
                     EIQNDNQP"
     gene            complement(46664..>46880)
                     /locus_tag="PLE39_03030"
     CDS             complement(46664..>46880)
                     /locus_tag="PLE39_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67324.1"
                     /translation="MILAGSAETDSNHTTMKNPPKDGHNGLAALLKTIFLLIAFLLSL
                     LFLKENPDLGMAFQEWKQHKRKKEISK"
BASE COUNT        10953 a        11078 c        12545 g        12204 t
ORIGIN      
        1 aaagtgaaat ggcgatcgcc atggcgcgtg aaggcggcat tggcatcatc catcgttttc
       61 tttccattga cgagcaggcg cggcagatcc agcgcgtaaa gaaggccgag tcgttcattg
      121 tagataaccc gatctcaatg agcaccgccc acaccgtcgg cgacatcaaa aaactggtgg
      181 acgaaacagg cgcaggcggc attttgattc tggatgcgga agaaaaactc gtcggcattg
      241 tcaccacccg tgatctgctt ttcgaaaacg atgacaaaaa atccgtcact gatttgatgc
      301 agaagaaggt catcactgcc tcgcccgata tttcgctaaa agaagcggaa ggcctgctgc
      361 atgaacaccg catcgaaaag ctgccgttgg tcaatgccga tgggcgtgtg gcagggctgg
      421 tgaccttgaa agacatcatg aagatcacac agtacccgaa agccaccaaa gattcccgcg
      481 gacggttgat cgtcggtgcg gcggtgggtg tgcgtgacaa ggaaatgcgc cgcgtagaat
      541 ccgcgctgaa agcaggcgca gattgcatcg tggtggatat cgctcatggc gattcaaacc
      601 tcgaaattga tatgatcaaa aatatccgca agcattttcc tgcggcgcaa attatcggcg
      661 gaaatgtcgc cacggccgac ggcacgaagc gcttgatcga tgcaggcgtg gatgcagtaa
      721 aagtgggggt tgggcctggc tcgatttgca ttacgcgcat ggtggctggt tcgggcgttc
      781 cgcaattgac agcagtgatc gaatgtgccg aggctgcccg ctctgccgga atccccatca
      841 ttgcagatgg tggcatccgc cagcccggtg atgtagccaa agccatcgcc gcaggtgccc
      901 agacggtgat gatcggctcg atgcttgcgg gcaccgatga aagccccggc atggtgatga
      961 cccgccgtgg tcatcgctac aaagcttcgc gcggcatggc ttcacgtgaa gccaacattg
     1021 accgcaaccg caaggaaggc aatgacctca cgcaggagga aattgaagag tatgttgccg
     1081 aaggtgtgga ggccgccgtg ccgtatcgcg gcagggcaag agaagtgctc acacaactgg
     1141 ttggtggttt acaatcaggc atgagttaca gcggtgcgca caattttgag gaatttcaaa
     1201 agaaggcaat ttttgtgcgg atgaccgggg cggggctcaa ggaatctggt ccgcatgatg
     1261 tggaagtgct tggttaatga tgaattatga aggatgaagg atgaataagg aaaaagtcga
     1321 ttacgctttt aaactttgag gcctttgtgt cctttgtgtt taatattttt ctttggaggc
     1381 caatatgaca aaccaggaaa cacagaattg gatcgagaaa gacaagcgac agttacaccc
     1441 gacctatcac ccgaaatcac atgccaatcc gttggtgatc gaacgcggtg acggtgtctg
     1501 gctgtacact accgatgggc agaaaatcct tgatggcatg gcggggctgt ggaatgtcag
     1561 cgcagggtat ggccgtgagg aattggcgaa agccgcctac gaacagatga aggtgatgtc
     1621 tttcacgtcc aacttctcgg gtatgaccaa cctgccttcc attacattgg ccgataaact
     1681 ggcagggttt gcttatgagg gtttgaatac aacctttttc acttccggcg gatcagaggc
     1741 gaatgattct gcattcaaga ctgcgcgcta ttactggaag cgcaaaggca aacccggtaa
     1801 atacaaggtg attgctcgca agggttccta tcatggtatt acactggcgg ccacctttgc
     1861 caccggcctg gagaaatatc acaccatgtt cggcccggcg atggatggat ttgtccatat
     1921 tccggcgccg aatccgtatc ggtacgaggg gcagttgaag gcgggtgaga ccgtcggtca
     1981 ggcggcggcg agagaactcg aagaggcgat cctgcgtgaa ggcgaagata cagtcgctgc
     2041 attcatcgct gaacctgtga tgggtgtggg cggtgtgatc gttccgcctg ccgagtattt
     2101 cccgcttgtc cgtcagatct gtgataaata cggtgtgctt ttcatcgcgg atgaggttat
     2161 caccgggttc ggccgcactg gcgaatggtt tgcgctcaaa cattggaatg tgaagcctga
     2221 tattctttcc tttgccaaag ccgtcaccag cggatatgcc caactgggtg gcattcaaat
     2281 ttcagatgag atccgcgaga ccatggaaaa taccgctgat accgaagcct acatgcacgg
     2341 ctacacttat tcggggcacg ccatggcctg cgcggttggt ttgaagaaca tcgaaatcat
     2401 ggaacgcgag gaatttccca aacgcgcccg cgaattgggc aagcgtttgt atgatggctt
     2461 gcaaagcctg aaggaatttt ccttcgtcgg tgatgtgcgt ggattggggc tggtctgtgg
     2521 tgtggaaatc gtttcagaca aagaggcccg cacaggcgat gccgcaaaga ccgcacagat
     2581 cttcaaggcg gctcaagacc gcgggctgcg cactcgtccg cttgggagca cgcttgcatt
     2641 tgcacctccg ctttccatca acgaagatga agtggatgag atcatcaagc gccttggcgc
     2701 cgcaatggat gcttctgcat agaaggttgt aaattacagg ttgcaggttg aagattttct
     2761 ttcgacctgc aaccttcaat cttaaaccag cgatttgcca tgtctgtcta tcttcatgat
     2821 attccccttg cccaggcccg ggctcgaata agagaagctc tgcaagaggc aaatttgtgg
     2881 cgcgtacttg gcgttgagga aattccgctc aacgaaaacg cgctgggacg tgttactgcc
     2941 gagccaattt gggccgaaat ttcttcgccg cattatcatg cctctgcgat ggatggtttt
     3001 gccgtccgtg cgatctctac caatggcgca atgccttcgt cgcctgttac gctccacgtg
     3061 gggcctgaag cacaatatgt ggatacaggc gacccgctcc ctgagtggac gaatgctgtc
     3121 attccgatcg aaaatgttga atcgcttgac gagaacgacc agatcaccaa agagattcgc
     3181 aaacccgcat ccattcgcat ccgttctgcc gttgcgccgt ggagtcatgt gcgcccatta
     3241 ggtgaggata tcgttgccac gcaactggtt ttacctgctg ggcacgcgct tcgacctgcc
     3301 gacctcggtg cgatcgctgc ctctggacat cagacgatcc gcgtcgcact taagccgaaa
     3361 gttgccattt tgccaacagg cactgaactt gtcccgatcg ggtccaaact aaaatcggga
     3421 gatatcctcg aatacaactc ccttgttctg gcggcgcaga tcaaacaaat gggcggtgaa
     3481 ccgacccgct acccgatcac cagggatgat tttggcctga tctgcgaacg cgtatttgaa
     3541 gcctcacagt ctcatgacct tgtgctgctc aatgctggtt cttcagcagg tgcggaagat
     3601 ttctcatcca aagttgttga aaagcttggc acactcctcg ttcatggcgt ggcggttcgc
     3661 ccggggcacc ctgtaatttt aggaatggtt aatcgtcaat cagaaaacct aaaacctgaa
     3721 accttgaacc tgattcccat cattggtgtt cctgggtacc ctgtctctgc ggctctgact
     3781 gtggatatct ttgttgaggc gttgatcgcc aaatggttgg gacgccctcc aatggaatta
     3841 cccactgaaa ctgccactct cacacggaaa ctcgtttcac ctgcgggcga cgatgacttt
     3901 gtccgcgtgg cggtggggca ggtgggagat aaattgcttg cggcgccgct ttcacgtgga
     3961 gcaggggtca tcacctcact ggtgcaggct gatggattgg cgttgatccc cagtggcgtg
     4021 caaggcatgg aagcgggaga gcccgtcaaa gtacatttgt atcgcagcaa agcacagatc
     4081 gagaaaacga tcttctgcat cggctcgcac gatatgaccc tggatctgct ggcccagttt
     4141 ttagccgaac atgatcggcg gctggcttca gcgaacgtcg ggtcacaggg tggcttggtt
     4201 gcgcttcgtc gcggcgaggc acacatggca gggtctcatt tgctggatcc aagcgacggt
     4261 tcgtataaca ttgcctacat ccgccaatat atgccgaata ttccggtgaa ggttgtttca
     4321 ttggtgggac gtgaccaggg gttgatcgtt aagaagggaa acccgaaggg aatcaaaagc
     4381 ctgggagacc tgaccagtca cgaggtccag tttgtgaatc gccagcgtgg ggcaggcaca
     4441 cgcgtcctgt tggattatca cttgaactta atggggatgt cctccgaatc catctctggt
     4501 tatactcagg aagaatatac tcatttgggg gttgcggctg cggtggcttc agggcgtgcc
     4561 gattgcgggt tgggaattgc cgccgctgcc caggcgttgg agttggattt tgtgccgctc
     4621 tttcaagagc gctatgatct ggtaatgccg aaggtttttg cggaaagtga attgctcgcg
     4681 cctctcttcg acctcttgac tgatcctgtt tttcgaaatt ctgtatcgaa attaacaggc
     4741 tatgatgtaa atgtaatggg tagaataatc atggaggact aatttcatgg aagacggatt
     4801 gatcatcgac gacaacagac agacagcaga tgcccttcaa caaatgttag gggtgttgga
     4861 tctacccgcc cgtgttgcat atggttcaag cccggcaatt tcgatgttga gcagttttgt
     4921 gccaggtttt atctgccttg atatcaatat gcctggtgtg gatggcacgg aggttctggc
     4981 gtttctccgc cgcgaacccc gcttgatgaa gattcccgta attgtcatca catcagatga
     5041 ccagcctgag actcgtcaaa gagtaatgcg cggcggggcc caggcggttg tcattaaacc
     5101 cgctacactt gacgctctgg agaatgcctt taaacgttcg ggtgttcttt cataatcaaa
     5161 tattttgcgt aatatgggtc gaaatgaaaa aagccatgac tgggaatatg gattcctgca
     5221 tggctttttc tttttaaaaa attaattcag gattctgccg atgaaccaac aggaaaaaac
     5281 cacgatggct ccagatgaaa aaacgggcgg ttggaagacg gggtgcttga cgggcttttt
     5341 gtcaggcggt gttggtgtgg tgggcggagt ggcagtagct ttgataagcg gctattttgg
     5401 gtttgactgt tttgccgatt cgcagttttt tatcgaagtt ttcattttcc ctgtggttgg
     5461 tttgctggca ggcgcgatca ctggggcggc agcctggctt gggattacat tgagcaaagg
     5521 aaaagcatcg ccactggtgg ttggcgggat catatacttt ttgttcttct gtatccttgc
     5581 cgtcatcgtt gctgccgttg caaaggcatt ctcctgctaa ggcgcaggcc agggaatttg
     5641 tgctggcttg gagaagccat gcttttcaac cgtgtataaa acgccttccg agggttgcgc
     5701 acagcctgct tcatcctgta cggtgtagct ggttgtaaat tgatcagggt aggaaaaggt
     5761 ccaccataaa taggcgccgt tttgacagac aaaagcaata atgagataac cacggtcgtg
     5821 gtcggtggtc atttctactt gattccagct gatggtgact tcgtcaccgt tgcggatggc
     5881 aacgatgttt tgcggcgggg gatagttgct gtaaccgact gattgcagat ctggctcgtt
     5941 atagtacagg gtgctgatat cgccaaccac atccacaacc gatggggcga cccaacagta
     6001 atatttcaat ttgtcgaatt ttacgtgtag ccatgagctg tattcgtatc tcccatcaac
     6061 gtaccctgtg tcaccggggt acaagtcggc ggcatgtaaa aatgccacgg aaggaccata
     6121 acgacagtgt gcctgtttat tgaccgtgac actgggaaac gaaaaggtcg gtgtggcggt
     6181 gatggtggga gtgaatgtaa tggttggtgt gagggtgagt gtggatgtta gtgtggcaag
     6241 cggggtggct gtgaattcgg gtgtgttggt aaaggcgggt gtttctgtag cagttggtgg
     6301 gagcggggtt gctgtgggcg cttgtgaagg caggctgcaa ccaaaaacga tgacagcaca
     6361 gatcaggtag ttgataagac cttttctcat ttgaacttct ccaataaggt gactgtgtta
     6421 gttccaagcg cttcatttgc gtagccgcct tccatgatga tggcggtggg aaggttcagc
     6481 gcagcgatcc gttttccaat ttcactgaaa ccgtcacgtg tgacatggaa tgtgccaagc
     6541 ggatcaccgt caaatgtatc catgcctgtg gaaaggacaa ggtgttgnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnncct ttttgagaaa gccagtttgc cgccacagcc
     6721 gcgttgttga tgaaacaata tcctgctgca taatctttgc cagcgtgatg accaggcgga
     6781 cggcaaaggg cgaaggatgc attctgaagg atgagggagg aagatgccgc acttaaagca
     6841 caatttgcag aagcgagagc ggctttgtag gtcgcttcca cgatgcaggc ggataaatcc
     6901 atgatgtagt agccagcttt gcccaaaagt gaattcggca cacgcggctt gcgacgcaat
     6961 gcaaaggtgg ctggcaggaa tgcgttctgc tcgggcgagg ctgcaacttg agggtcagag
     7021 gccagccact cggtccaggc agaggcgagg aattgaatat agttcccgtc atgcacagca
     7081 agaatggggt caagtccgaa atcctgtggc tcggtcaatt ccgcccaatc cgtttttcca
     7141 agtgcagcca aaattctatc catacgatca gggttttcgt agtagggaat gcgtttgcct
     7201 ccgtcaaaaa cttcaaatgg cggataatgg ctgcggtgtt cttcagaata aaatattttc
     7261 atggtaagac aattttaccc gcaagcgtca gtgacggcgg aggaagcgat ggaactattt
     7321 gaagcaattc atggacgatt gacgatcggc aaggtgaaac cagaaccatt gccgcgtgat
     7381 gtgatcgaga agttgttaag tgcgggagcg caggctccca atcatcataa ggtaagaccg
     7441 tggcgctttg ttgtgttgac tggggacggg cgtaaaaaat taggcgatgt aatggccgct
     7501 tcttttctgg accgtaatcc tgccacgcca cccgaaggtt tgaacaaacc ccgatcattg
     7561 tcgctccgcg ccccgctgtt gattgctgtc ggtgcggata aacccgccgc tgattcaaag
     7621 accattgaag ttgaaaatct atcggcggct tctgcggcct gtcagaatat tttgcttgcg
     7681 gctcatgcac tgggacttgg ggcaatgtgg cgcacaggtg aatgggcacg ggatgccagg
     7741 gtgaaggagt ttctgggttt ttccacagac caacatatcg tcggctttat ttatgtgggg
     7801 tatccagaag tgacgccaga accgtacacc cgtattgggt ttgaggatcg aacagtttgg
     7861 atggagtgat ccagaaaaat aaagatgaaa aaagagagta acgaggctga cctcattact
     7921 ctctttttgc ttaatcgtga tattttaatt cgcctgcctc cactttgatc ggcaggcggt
     7981 tttctctggg tggaatggga caggtggcat aaggcgtgta ggcacacggc cagttttcag
     8041 tgaggttgaa atcgaggacg atttcatcgc ccttggggat gggcaggtaa aattttcgcc
     8101 cgccgccata ggtggtatct ttattcgtgc catcggtgaa attgaaaagc aattcgtcgt
     8161 cgcttttttc agcttcgagc acacattcaa cgccatttaa tgtgaaacgt gcctgaccta
     8221 aaaatgcgct ctcaacttct gtgccaatga cttccgtaat tttgatcggc ttgggtgggt
     8281 catagcgaac gtattttgcg atgacgcggt attcgggttt gggcgggtaa taatgaaatc
     8341 catcgaattg tttgagcgca ggtgattccc gatcccagat ccgcaccaat ggctctccgc
     8401 cgcggatgat gattttcagg gtcagtgaac cgatgctgat caaatcgggg tctttttcac
     8461 ggtcagtgcg caatggactt gcgtttgggg tctttccgtt tatagtgata tcagcgcctt
     8521 tggcagggtg cagggtgacg attccgtttt cgaggcggaa ggagccgcaa tgggcacttg
     8581 gaaaggcggg caggcagatc ttgttgtctg tgccgtttcc gaaagagttt tcgccttttt
     8641 ccagccagaa caggcctgcc agatttaacc agtgaagctg gttcgctgct gttttttcgt
     8701 ctcgttggat gcggtggttc tcgatgctgt cttcgtagga tgtcattgcg ttttaccttc
     8761 ccaatacctt tatcataata tctggcctgt cggtgatgat tccatccacg ccccaggcga
     8821 ttagtttttt catggtttct tcatcgttga tggtccacgg ctcaacagcg aggttccgct
     8881 cgtgggcggc ttttacaaag gcaggagtca ttacggtgat gccgccagat tcttcgggca
     8941 cttgcaggga gaagaatttg ggtgagtaaa aactgccaag aaacggtttg gtgagcagaa
     9001 caaaaaccgt cgtttcattt tttgcgctgg aggttgccac ttcggggcat tccttgcgga
     9061 attccttgag tctgtcgtca tggaaggatg cgatcaagac cttgtcttcc atgttgtatt
     9121 tacggatcaa ttcgcaaaat ggtcctgcca ttgaagcatt tgtctttttg atctcgatgg
     9181 tcatgtgctt gtctgggaaa gcagcaaaga cctcttccat ggtggggatt gtgacaccct
     9241 gtccgcgaaa ggggaatgtg gcgccttcat cgggcgtcca gtcatagcct gcgtcaagct
     9301 tcttgagctc gtccagcgtc atggattcga tctcgcctgt gccatttgtg gttctgtcca
     9361 cagtttcgtc atgcatcagg acgagggtgc catcctttgt gatgtggacg tccatttcga
     9421 gcacgtctgc tccgaggtca acagcgtttt gaaaggcaaa catggtgtca ccgggccaga
     9481 ggccatctcc gccttggtgg gcgatgacga gggggtaatt caggtctgcg gcgtagtagg
     9541 gatgatccac tgcgggtctg gatgtgacgc ggaggaggat ggtggcaatc aatagaatgc
     9601 ccagaatctt ttgccaagtt ttgagtttca tctgtacggc tccgatgtaa gtgtgtgcta
     9661 ttttaccctc agaattgaca tgcacagcct tttcacttat aagtatcaaa tcctgtccgt
     9721 agatggtgtt tttcggatgg ctgaaaataa atttctggag gcagatatgc ttgcgttgca
     9781 gttgttatct gagggtgggc atgggccaga cacttccctg tcatggctgt tgtatattgg
     9841 aattgcgttc ttcgtcatga ttattgtctt cgggtggcct gaaggctcaa agaaacaggg
     9901 gcaggctgaa ggtccgcgtg aagcgccgaa agggaagaaa aaagccgatg accttgacta
     9961 agtttgaggg aattggtcca taagttgcca aggtcataaa taagaattga aatgcgaaat
    10021 aaaaaaacac ctgcccgggt ttcttcaggc aggtgtttgc tttagtttat taaatgaata
    10081 gatagcggga gattgccatg aaaaccactg cggctaataa tgcatagatg ttgatggtag
    10141 agaaagtgcg ttgtcgcttt tgcaggtctt gaatttgtgc gatctggtct ggggtgggct
    10201 ggcctttgat ctgtgagccg atttgcatca tggccatgtt ggttttgcca attaagaaac
    10261 caaataccag gccgatcaac gcaaaaaatg ctccgatgcc gaacccaatg ccggcgttcg
    10321 agctgaacca tgtgggtgga ttgcggacat agaggatagt tcctgcaatg acggttgaga
    10381 gggaggaagt cgtcatgatt ttctgaaaat tggtttttcc catcatctgc cgtaaaaatt
    10441 gttgtccggc gtcgccaaca gctgttgcgc tgggcacaat atacagtgtc agcatccagg
    10501 cagcgccgac ccaaaataca cctgccacga tatgaataag ccgaagtact aaagtcaatg
    10561 cattcatgat tttctccttg ttataagtgg tttggggtta taaccccact tttctgtcta
    10621 cgtgttgaaa tggcgaaaaa agggtgtgtg agagggggat taaaatcctg tacttaaatt
    10681 cacaaaatgt atataatacg ccccgctgtc gaaccaaata tacatacgga ggtagtaatg
    10741 aagaaagttt tgtttgctgt tttagttttg gctgcgctcg ctctggcagc ttgtggtggg
    10801 ggctcgaatg cggtagctac tttggctcct gttcctgccg aatatgcagg tcaggccaac
    10861 cctcttgccg ctgacgccgc tgcggctggc gctgaagtat tcaagaccaa ctgcgaatcc
    10921 tgccacggcc cgcaaggcca cggtgatggc cctgccggtg tggcacttga ccctgctccg
    10981 aagaatttgc ccgaacttgc cgcccaggct ggtgatgatt atctgtattg gcgtattaat
    11041 accggtaaag aaggcacatc catggttgcc tggaagggtg ttttgaccga cgagcagatc
    11101 tggcaggcga ttgcgttcat ccgcacgctc aagtaagtag atcagggtat taaacaaaaa
    11161 acggcatgct ctatgcagag cgtgccgttt tttgtttgca tgtgtataat acgaccctta
    11221 taaaaaagga gattaaacca tgcccatccc atcaggaatt cccgcacccg agtttgaaat
    11281 gcttgatgat acaaatgcct tgcgaaagct gtctgagttt cgcggcaaga atgttgtttt
    11341 atatttttac cccaaggatg atacaccagg ctgcaccaag gaagcttgta atttccgcga
    11401 tgattattct gcttatgaaa aggcgggggt ggtcattttg ggggtcagcc ctgatgatgt
    11461 ggcttcgcat gtgaaattca aaaagaaatt tcagcttcaa tttccgctgc tggctgatga
    11521 tggtcacaag gtttgcgatg cctatggtgt gtgggggcca aagaagttca tggggaaggc
    11581 ctatgagggc gttttgcgta caaccttttt gattgatggc gaggggatga tcaaaaaagt
    11641 gtatgagaat gtccgccctg cggaacatag tgctgaactg ttgaaggaat ttggtgttag
    11701 ctgaggttgg tcgcattttt gcttctttgc tgatcctgtt tttcggacgg agccatgaat
    11761 cagattaggg aagatgcgta caatgtccac aaaaaatagt gacaatttga ccaaaatttg
    11821 gtaaattagg tgagtttggg gcgggcatcc ccgtttgaat ttttttgagg agagagcata
    11881 tgcgacactt tgtgatcgtg ggaattttgg tcattgccat ggccgttctc acttatgtag
    11941 gtcttgacgc tgccggtttt gcaacacaga tgaaccctgt tgcagcaagc gctcaggcgc
    12001 agacaatcga taatatgtgg cactgggaga tcgttgccat gtctttcttg tttgccttga
    12061 tcgttgcgcc gatggcgtac agcctggttg tgtttcgaaa gcgcaagggc gatgataccg
    12121 acgcccagca cgttgaaggt aacacagctt tggagatcgg ctggaccgtt ggtccgttga
    12181 tccttgtggt tatttttgcg tatatggggg cgtattcgct tggtgaagtc cgccgcgtag
    12241 atcctgaggc ggttgtcatc aaggttaaag cgcagcaatt tgcgtggaca ttcgaatatc
    12301 cggaatatgg ggtggtgagc aacgagttgc atttgcctgc gaataaacag gtgttgctta
    12361 agatggaatc tgctgatgtg attcattcgt tttgggttcc tgaattccgt gtcaagcaag
    12421 atgttgtgcc tggtcgtgtg accgagtaca gaatcacacc gacgttgatc ggtgtctata
    12481 aagttcgttg tgcggagtta tgcggtacct ctcacgctta catggaaggt atgattgttg
    12541 tgagcgagga agccaattac gaagcctgga tcgccgagca aattgcgatt gctgccgcag
    12601 ctcaaacccc cgagggcaag ggtcaattgt tgacagtttc gaagggttgt gtcggttgtc
    12661 actccattga cggcactgcg atgacaggcc cgacatggtt tggcttgttc ggggcggatg
    12721 tgaaactggc ggatggttcc actgtgacgg ctgatgaagc ttttatttcc gagtccattc
    12781 ttgacccgaa cgcgaccatt gtggaaggct tcccgtcccc gtctgtcatg cctccgtttg
    12841 cattgaccga ggaagagatt gcaaacatca tcgcgtatct taagacgctg aaataatctg
    12901 gcggaggatt tcttatatga agcgtttcat ttcaagtgct ttggcacatg gccttttggg
    12961 gcagttgatc ggcaccgtgg tgggcggtgg gctggtcact ggtattcgtg cgctcatggg
    13021 gttgagcgtt accgaccgtt ttttcttcac tgaacctgcc tgggtgctgg gtggttttat
    13081 cggcgccatt gcctttctgt tcagttccgg ggttaccagc gattgggtca agtgggcacg
    13141 gggtattgat acccctgaac atcacgaaga ccatcactct ggctgggaga agttcgtcaa
    13201 tgtttctctt gaccataagg tcatcggaat ccaatatacc attgtggctt tggcgttgat
    13261 cagcattggt ggtttgttcg ccctgatctt ccgcacagaa cttgcggcat ctgaacttca
    13321 attcctgacc acagatatgc aattgtttgg tcagaatggt ccgcagcttt ataacacgtt
    13381 gatgtctttg catggcatga tcatgatcgt gtccattttg ctgggcattg ccggtatcat
    13441 caactatgtt gtgccgctgt tgcttggcgc tcaagacatg gcttttccgc gtttgaatgc
    13501 gttctcgttc tggatcgctg ttcctgctgc cgttttgctg ttgatgagtc tcttcctcgg
    13561 cggttttgat acaggttgga ctggttaccc gccgctttct gcccgtgccc ctgttggcat
    13621 gcagatgttc ttcctgggag tattcgtggc aggttggtcg tccattttgg gcgcattgaa
    13681 tgtgatcgtg accgtattgc gaatgcgcgc caagggtatg tccgcaatgc ggatgcctat
    13741 tttcgtttgg gctgcaattg ccacctcact cattgcgttg accgccaccc agttcatcgg
    13801 tctgtccttc cagttggtca tgttccagcg ccttttcggc atgggctttt tcgaccccgc
    13861 caaaggtggt aatcctgttc tcttccagca cctgttctgg ttctactcgc accccgctgt
    13921 gtatgtgttc attctgcccg gccttggtgt gatttctgag ttgttgcctg tattcgtccg
    13981 caagccgttg ttcggttatc gctggatcgc catgtcatcc ctcggtattg cgttggttgg
    14041 gtttgtggtt tgggcacatc acatgttcac ttcaggcatg aacgaatacc tgcgcgttcc
    14101 gttcatgtac agcacccttc ttgttgccat ccctacgggc gtcaagttct tttcgtgggt
    14161 ggccaccctt tggggcggca agatttcgac ccccactccc atgctgtttg ttttgggcgg
    14221 tatcgtcgta ttccttatgg gtggtctttc tggcccgcca aatgcgatcg tttccaccga
    14281 cctgcacctg catgacactt acttcatcgt tggtcacttc cacgatacca ttttcggcgg
    14341 ctttgtcttc ccattctttg ccgccttgta ttactggttc ccgaaggcca ccggtcgccg
    14401 catgaacgaa accctcggca aggtacattt ctggctgatg accccctcgt tcttcgtttt
    14461 gacctttggc atgatgtatg tgggtctgtt ggggatgcgc cgccgcatcg tggactacga
    14521 cccggcgctt ggtcttgatg caacacacct tgttctcacg atcgcaggtt acctcattgc
    14581 cctttctgtg gtgattttca tctacaactt cttcagcagc atcaagaacg gcgaaaaagc
    14641 cgagggcaat gtctggaatt cccgctcacc cgagtggcag gttccttctc ccatgcctgt
    14701 acacaattat gaccagcctt tcgaagtaat cagcgaacct tatgattacg gtttggaagg
    14761 cggacatgcc tacatcaaat tctccgcgcc tgaatctggc gcgcacaagt aaaggggagg
    14821 agcgcaatgt cacatacaca aaccaaatct gccgcttacg gtcagggcgt ggtgattttc
    14881 atctaccttg ccattttgac cgctctggaa tatttcgtcg ctattacctt caatgcggta
    14941 tccatccttg tggtggtggc tgtcattaag gctgctcttg tcatgtacta ctacatgcac
    15001 atttacaagc tcaatgaaga cagtgaaggc gatgaacact cctacgatta caaaacgggg
    15061 acgaaccgca tcggtttatg gctgttcctg ctctcggatg gtttcgtatt cgcaggcctg
    15121 ttggtcaccc gcatcaacct gctcgggttg acacgtccac acttgaacca gaccctcggt
    15181 ctggcggtga cggcggtcct gctggtctct tccttcttca tgaaccgcgg cgaaactgcc
    15241 atggctcatg gtgacaagaa gggtttcatg aacaatacgc tcatcacctt cgtgctggga
    15301 ctaggcttcc ttctcggtgt agtgatggtg gaatggcgcc tcgctgctga agaaggattg
    15361 accgccagct ttggcaaccc tgccgttggc ccgatgggcg gcgtgttcta tatcatgaca
    15421 ggtatgcatg ccttccatgt gttgactggg ttgatcttcc tgctgatcgt gtggaacaat
    15481 gcccgcaagg acgtctactc ggctgaaaag cactggggcg tggaggctgc cgccgtttat
    15541 tggcacttcg tagacctggt gtggatcttc ttctaccctg cgctatattt gatcggcaag
    15601 ttagtaaact aaaaaaggac cgccgccgag ttaggcggcg gttttttatt ggaaaaattc
    15661 ttcaaacttt gatataagcg tacctgttcg ttacacttgg agcaagaaca tatcatggat
    15721 aaacgcatca ccttcgtagg cctggcctca ctcggaatcg tggcactggc ctttgttttg
    15781 aatatttttt ttcttaaacc agcgagtttt cgcggtgctt tgtatggcga accgtacccg
    15841 cgggcctcag agatcgaatt gcctatggcg aatggacaaa cattccgcct gaccgaacag
    15901 catgggaagg tcattctact ttttttcggg tatacctcct gcccggatgt atgcccgacc
    15961 acgcttgcag aaatgaagat ggtgatggat caattggggg atgatggttc gcgtgtgcag
    16021 gttgtgttcg tttctgttga ccctgaacgg gacacccctg aaaaaattca aacatacgtg
    16081 gagcacttta acccagcgtt cattggtttg agcggttcct cagagcagct tgagcccgtc
    16141 tggcaggcat actcgatctt ccgcgaagcc gtgcaaagcg actcggcttt tggcgtcata
    16201 attaatcaca ctgcccgcct tttccttgtg gattcacagg gcaatttgcg tctttcctat
    16261 gcctaccaaa cccctgttga tgatattgtc cacgatatca agctgctatt ggagcgggaa
    16321 ccatgaattt ctccatcaaa cgaatagtaa tttcatttct cctcgggctt gtattggctg
    16381 ccattaccac cgaggcggct tacttttttt tgaaaaagga aaaccgcgca ccaggactca
    16441 ttgaattggt gatccccgct ggtactgcag attcgatccg cgctgggaat gcgccagtgg
    16501 aaattcccag agatatgcgt tttgttgtag gcgatacttt gcgcgtgatc aaccaggatg
    16561 atgagaatca tcaactgggc ccgttgtgga tccctgccga gtcttcggcc agcctcaaac
    16621 ttgaaaccgc cgagaatttg atctttgaat gctcatttca agccgacaat aatttcgggg
    16681 tgacagtgat ggagccagtt acctggcgca cgcgtgtttc agggattttc tttgctgggt
    16741 ttccgcttgg catgatgttt gccgtatatg gcggattgat cggagagaag aagaaaaagg
    16801 ataacgagaa aaaagatgat ggttcttcgc aaggcaatct cgcgtaaatg gttttttgcg
    16861 tccctgctcg ttttgctggg tacggcgttt tgcatccgtc ttgggttttg gcaattggac
    16921 cgccttgaac agcgccgtgc ttttaatgcg caggtcgaat ccatgcgtgc ggcggaaatg
    16981 ctggatttga atcaggcaat tccgcaggat atcgctgcga tggaatggcg tgcggtcacg
    17041 gtgacgggtg aatatgattt cgagaatcag gtcgccatac ggaatcaata caacggcgat
    17101 caatatggat accacctcat tacgccgctt ctttccaacg ggacggctgt tctggtggat
    17161 cgcggctgga tccccgctga tggaaatgcc acacccgccg attggcgcca atatgatgag
    17221 cagggcactc tgactgtaac agggcagatt cggcttgggc atggtaaacc agccatcggc
    17281 ggaatcgcag atgcgctgcc tgcggcgggg accaggcttg aggtgtggaa taatctcgac
    17341 gtggagaata tgtcggcgca gtttccttat cccattcttg gggtttatat tcagccgaat
    17401 gtggagcctg tcgatgaggt tccgcccatt ccatatcagc caattattga gttgaccgaa
    17461 ggttcgcact tcggttatgc tttgcagtgg ttcacttttg cgaccattct atttgtcggg
    17521 tatccatttt atttgcgaaa gcaggaatag caggtatgaa gtattctctt cgttcatctt
    17581 ttgcgcgttt gacattgtcc ctgttcatca tggtcttttt gctgaccatc gtagggcgga
    17641 tcgtgacggt gacgggtgca tgggcatatt gtttgggctg gcctttgtgt gtgccttcag
    17701 agccgattgg gtatttgaaa ctggcccatc tgattctggt gggattggcc atggtgttga
    17761 tgttttggct gctgcgcaag gcgtggcgcg aacagcgcca tcagcaggtg attttgccgt
    17821 tgaccactgt gacaggcgtg ttgttcttcg ggcaggcttt catcggggca atcgaagtga
    17881 cccgttccta ccccacacat ctggtggttt tgcatgcttt gaccgccgtt gccctgtgga
    17941 tcagtttggg ttcgctggtt tatctttcgg gagccctggc ggtggattca aacgaagtgg
    18001 tgaagtttga ttaccgtcaa cgcgcgaagg attttttcat tctttccaag cccattattg
    18061 ttttgttgtt gctggtgacc acctacagcg gattggtggc tggctcaaag gcatggcctt
    18121 cggcttcgct gacattctgg accctgttgg gcggtacgtt ggcggcggcg ggctccagcg
    18181 cgttgaatca atacattgac cgcgaactgg ataagaatat gcagcgcacg tccaagcgcc
    18241 cgcttgcaga tggacgcctg accgcagcgg agggactttc ctacggcctt gccctgtgtc
    18301 tgaccagtta ttatgtgatg gcgggtttcg tgaatttatt ggcggccctg ctctctctgg
    18361 ctggtatttt ctattatgtg ttcttttaca gtgtctggtt gaagaaggcg actgtgcaaa
    18421 atattgtgat cggcggtggc gcaggggcga ttccgccgat ggtggggtgg gcggcggcga
    18481 ctggtcaatt gggactcgca gcctggatcc tttttgccat agtcttcatg tggacccctc
    18541 cgcatttttg ggcgctggcg attgtacgta tgaaagatta tgaacgggca ggtgtaccca
    18601 tgatgcccgt ggtgcatggg gaagaaaaga cccgcaggca tattctggtt tacaccattg
    18661 agttgatcgc ggtcacgttg ttgcttccga tcttcaacct ggctgggatg gtgtatgccg
    18721 tatctgcgat cgtgctggga ggcttccttt tgtatgctgc ctggcgtgtt tttaaagtgg
    18781 gcggcaacaa agtggcatgg accatgtacc gctggtcgag catgtatctg gcgtttattt
    18841 ttctggcgtt gatgatcgat gccgtggtgt agaagtgaaa atcctccgag aacctcggag
    18901 gatttttttt acggcttttt cacgtagaga tagatcctga catcgttcca gttttcaata
    18961 aattcgagtt cgtaattttc ttcgaggcct tcaagatgtg gatgggtttt gtatccttgt
    19021 gctgtgtatt cgtccacaga ttgttggtag atgatgaacc agatccgcgt ggcatttgcg
    19081 gaggctgttt caatgtcttc ggcagagcgg atctgcagta cctcacgcgt ggctggggca
    19141 agtgtgtcta cattgctttg gggtgggtca atgatgaagg tttgaaccag cttgcggtta
    19201 aagtagaacg agggcagata ggaaagtttg ctggagtgaa tgatcgcatc gccgttttcc
    19261 aatctgcttt gaatgctttg gttgatttca cggaaagggc cgtagggaaa accctgatag
    19321 aagaaatgct gtgagagccc gatgacggca gcgataccga taagcgtgaa cgtaaaaatt
    19381 cgcacagggt taggcagcct tgtttgagtg aacgcccatg ccagccagat acaaaaaatg
    19441 gcatgggaag gcagaagggc cctttcgata taaacaggga caaattggga gatgacccac
    19501 aaaagcagag gcgggagaaa tgccagcgtt accagccatg tggccttgcc tgtattggcg
    19561 atgaggtttc gttttgcaag gatggtttgg aaaacagcca gaacgatcac cagcatggcg
    19621 atcagcagcc ctggcaaaag cagtccgttg ggcaggggta gatgcggaag gtagaacaga
    19681 atcagtgtaa agattttttc cagcccgggg cgctccaccc aataagaaga ttgtactttg
    19741 gaaagctgag cgggcagatg aatgagccac ggaatgtaaa gcaggacagc cagcaatgcg
    19801 cctgccatga ccgatttgag cgttttccag tccttttgcc acagcggcgt aagggcaagt
    19861 ggaatcaagt acacacttgc aagatgatgt gtgtattgtg ccagcgcact gcaaacccca
    19921 aagagcagcc accatttcca ctcgccgccg cgtccgcgca gaaaggcgta ggtggcggcc
    19981 agcagccaga acgagagcag catatacatg cggatttcct gagcgaagtg gacctgaaac
    20041 ggaaggatgg agaagagcag tgcggaaatc caggcggtcg tttcatcgaa taattcgcgt
    20101 gccatcaggt agaccaggac cagcgcggca agactgatga aaatggatag caaacgtgcc
    20161 gcaaggatgg acgtgccgaa gacccgcatc catgcccata gaaaaacgta atatcccaac
    20221 gggtgaatat cagctgcgcc agaaccgctc tgactcagcg taccgtagat cattgcgttc
    20281 agtccctttt cagagaataa aatcgagaac gcttcgtcgt accaaatggg gcgtgtgaca
    20341 agccctgcca agcgtgtgag cagaaatatc accagcacaa gaattagttt cgctttttga
    20401 cttttcattt gacccgatac actttcacag aagttgagga atataccagt gccgcgtttg
    20461 cctgcgcgtt caagtcgctg cccaacgggc tttgccctcc agcggtggag atcaaaatat
    20521 aactgtattc atctgtcagc aggttttcaa gattgtggat gcaattcatg tcggcgtaca
    20581 ggcattgctg aatgtttttg accacgtcgt attgatttgc gtaactactt ttaccactca
    20641 gccattcggt tccttgaatc gtggcaacgc tgcgccgttc tgaaagggcg gggaaccatt
    20701 ccgtcagcgg ggagtgcagc ggattgcctt catcatccaa aaccaaaaaa cggtcggcag
    20761 ggtcggtgtt gctacggacc cattcgatcg ctgtccgttc ttccttcgtc aagacctggt
    20821 acgagagtgt gtttgacact tgaaaggcgt tgtacgtgaa cagcagaatg aagaagccga
    20881 agaacgatcg tccgctgtgt tgtttgaggg aatccatcca ttcagttgac ggggtcaacc
    20941 tcgtggcgat gccatctgaa agcaccgtca tcgccatgat cgaaaaaggc aaagtggatg
    21001 caggcaagcc gccgcgcggt tcggccagta aacccattgc cgcccatgca attgggagat
    21061 aatctttttt tgcggcgtgc atgaaaatgc cgcacagtgc aaaaaccgcg atcacgggag
    21121 tatattcatc agtaaacgac agcgtgaaaa agaccgtcca gatcttcaac cgcgaataac
    21181 taccctgccc tgcatggagc agtgtttcaa gcccgtgatg tcgtatcacg ttgaaccacc
    21241 atggcgatgt cagcagcaaa acgctgatgg cgactattac agcataaaac accccctgcc
    21301 gatctctgcc gaaaaaatac cacaggacaa gggaagcgac agccgcctgc acagcccagg
    21361 caggatgact caacacgcat cctgctgcgc tgaaaactgc cgcaacaacc caggaccacg
    21421 acctgcgctg aaacatttgg tgaacgcaaa aagcaaccgc tgtaaaaaag aaaacgccca
    21481 aagagcgtgt caacccgccc ccgactatgt tccaccagta ggcgctgggg gtgagcgcaa
    21541 agatcaatgc agccaatgcc gccttcggtt ttgattcgat caatcgttct gccatgaaat
    21601 aaaaaagcgg aatattcaac atcgaaatca aaaatggcag ccagcgaagg atgtttagca
    21661 ccgagatgcc tgtcaataaa ttgatcagcc ctgccagata aaagccaagc ggaggatacg
    21721 caaatggaat atccgcctga ttgtaggatg tgaccagtgg cagggaaaac ccttccgccc
    21781 gcaggtcatt gaccattgtg taaaacatgc ctccatccac cagtgggaag tctgttttga
    21841 ggatggggta caaccgcact gccagcccaa agacaactgc cagcagcagg aaaaatccag
    21901 tgacatcctt ttcgtgagat tgagcttgtt tcatggcctt ctttgataaa tgacaatttg
    21961 actgtcgctg tacacctgaa cccatgtttg cgggtcgagt ttttccaaca gggtgtgata
    22021 gggttcgagg aagatcaaat tgattccccg cgattcgagc cgctccagac cagtttcggt
    22081 cccgtttgaa atctccaccc attcctgaat gaggtcgttt ccatacaagt cggcacggcc
    22141 atcaatgaaa acgggatact cacgcaaaga ccaggtgaga tagccgcccc agttatatga
    22201 attgaagatg ggtccgctcg gttggtgttc tttgacccat gctacagcct gtgcaggaac
    22261 ttccttttcg atgcttgcag gtattgagac caaccaggca tatccaacga gcaggatgat
    22321 gaacattccg atcactgcat tattgaccac ccctgcaacc cctggcgaaa gctgtccttt
    22381 cgaagcttgt ctggaaaagc gttctaaaaa cgccttcacc tgcggcgctt cggtcagatt
    22441 tgcccatgcg ggggcaagga acttttccac gatgggcagg atgatgatgg tgtaggctac
    22501 caaattgcgc tgcgcgaaga agaacaaata agcaaatccc aatcctttga atagatcggt
    22561 gaaggaaagc cgttttcctg aaaacccgag tccgaagacc agcaaaaaca tcagccaaag
    22621 cattgggtgg gcatagatct ggtgaaagtc tggtgaaagc cattcttgga ttgccaatga
    22681 aacatccact tgctggaagg gtagtttcca aagcgcgaat ccgttcgggt tgagtgccac
    22741 ggcgaggaag gcggcgagac tccacaaagc cagatgcccg atctctttga atacaagttt
    22801 tggagaattt ccaaatagaa gatcgaacga ttcgcctgca agggtggcga acaagagcag
    22861 ggcgccccag atgaatccac catgcagatt accccatagc gcaaaaagca gaaccagttt
    22921 ccacagcggc tggcgtttgg tgtggatgtg ctcatataaa aatgaatcca gccatgccag
    22981 taacaaaaag gatagcaact gcggacgggg agtccagttt gcgattatcc ccatcatcgc
    23041 gagaatgacc agcaatccgc gcggaatgat cgcgttcgtc atccgcttga aaatgatcca
    23101 caaagtgaaa attgccagca gggcggtggt cagtgtgatg gcgaaaaatc cgccgacctg
    23161 ccacaggccg tagagggcaa ggtctgaaac ccagaatgca ttgacccagt ctgcgccttg
    23221 tttggtgtat gaaaattggt cggtgaggag gatgcggccc tgatgccaca tttcgtcgcc
    23281 agcgcgtaaa tgccaccaca tgtcattatc cacaggtgga ttgatggcga tggcggcgac
    23341 caataaaaat acgatggtga tgaaaaaacg ctcggttcgg gtcatggttt tgcggggagg
    23401 gctttgtatt gttttacggc gaagaacagc ccagccataa tcaggtaggg tatggaatgg
    23461 gtgaggatgg gcgaataata actgaataaa agcagaagtg atatggctag cggaattaat
    23521 accgcctgtg cggtttgaat ccgttgtttt tcaagcagga tgagaatcac cgccatgatc
    23581 ggtgcgatga gcaaaaccag atcctgcgcg tgggtgtgag gtgaaaagaa aagcgccgct
    23641 acaactgcaa gactgatctc ttctcccgaa gggcttgtgt gatttcgcca gaaataaact
    23701 cctgttgaaa aaaatgccaa tccgtaaaat gcccagcccg cgcttcggat cagcgcggcg
    23761 gagagaaatg ggaaaagcct tcgcaaaagg ccaatgaggt tgaccatgtt ctcttcgctg
    23821 gtgcggtagc cctcgccgct gccgctgacc gtgaggatgt gcaggaagcc tttcaaaccg
    23881 tccgtgccta caaccccgaa actgaataat gtcaacgcga agacgaaaag caggaaccac
    23941 ccaaaaatag cgcggcgttg aaaaaggaag ggaagcaaca gaaaaaacgc aaaatgcgga
    24001 cgcaccgtgg caagagataa ccccagccct gcggcccaat cctttttggc gtaggtgccc
    24061 cacattaata agctgagccc cagaaagatg atcgcattat cctgcccgag caggaggctt
    24121 tgaaagatgg gaaagaaaag gaagatgcca gcggcaaaga tcagggtttg aggttgtgaa
    24181 aaaaaatttc tggcgggcgc aatcaaaaac ggaatcccag ccgtgaagag gagcagcatc
    24241 aacattgccc agcgttgaaa ggattttaga taatctgcgt ttgaaacgat ctgtgcaagc
    24301 ggaatcacaa aaggcgggtg aatatacgga aaggtttcct gcaattcgat ctcacgctga
    24361 acaaccccct cttgatattt tttttgcaaa atcagatcat aggcatgaga agggccttcc
    24421 tggtctgcaa tgcgtcctgc cgcgtagaag gcaataaagt ccgcgcctgt gtgttgggtg
    24481 gggtccgcga gcatcttcag ccagagcagg ggatagacca gcgccaacga gatcaatatg
    24541 gcagtgatgg ctgttcgtga aagatcaatg ttgattttca tgggcgcagg gtctgggttt
    24601 cggtattgta gcagataata taaaagccct tgatctctca agggctttgt aatttattcg
    24661 cctaaatagt cgcgcagttt cctgcggatg gaggggtgac gcaggcggct gagggcctgc
    24721 gcttcgatct gccttacacg ctcgcgggtg acgcccatct tgcggcccac ttcttcgagg
    24781 gtgtaggcct gcccgtcgag cagaccatag cgcaattgca aaatacgcac ttcacgcggc
    24841 ggaagaccgt tcaacacttc gccgagatgc tcgcgcagca ggttgtaagt ggcggtttca
    24901 tcgggcgggg gagcctcatc gtcttcgatg aagtcgccga gcaccgaatc ttcttcatca
    24961 tcggttggcg tttccagcga aagtggacgg cgcgccacct gaatcatgtt ttccactttc
    25021 ttgggcggca catccagggc tacggccaat tcatccacgc ttggttcgcg cccaaggcgt
    25081 tgggtgagtt gatgctgcac acgcaggagt ttgttgatct ggtcgcccat gtgcacaggc
    25141 acacggatgg tgcgtccctg atcggcaatg gcgcgggtca cagcctgacg gatccaccag
    25201 gtggcatagg tggagaactt gtgtccgcgg cggtaatcga atttcttcgt ggcgcggatc
    25261 agaccgatat tgccttcctg aatcagatca aggaagggaa caccacgtcc catgtatttc
    25321 ttcgcgaccg aaatcaccaa acgcgagttc gcagtgatga gatgttcacg gcctgcccaa
    25381 ccgtcttcga tggaatggcg aagttcaaga cggcgcttgc tggtcacgtt tccatgggcc
    25441 agttcttcgc gggcggcgcg gccacgctcg atgcgttggg ccagttgcac ctcttccact
    25501 gcggtcaaca acggtacgcg gctgacctct ttgaggtata gaccaatcgt atcgtctgtg
    25561 tcgatgtttg ccagataatc gtcaagcgag gggtcgggta gatcttccgt ttcggtcgtt
    25621 tgctcaacag cggctaattc atcttcggtc ggctcggggg tgatggtatc ttccataaaa
    25681 gggataccag cgctcaacaa agccgaaaaa gcctcttcca actgttccac atcctgttca
    25741 gcttcaggga agaaatgcag gatgtcatcc agcgttacgt atgatttttg gcggcccaat
    25801 tccacgagcc gtgcgatagc tgaaaactct tcttcgtcat ctacaatcaa aatcttctct
    25861 acatccattt acatatccaa ctttcaatta tatttaccag caaactgcta aatcagaata
    25921 cactttttta acctgaaaat caatacctgc cctgttttca gatgctttgc aatgcttttg
    25981 tattacgaac gtccggagta tttccggacg ttcgcgttca gcagttgtaa ttaccccatt
    26041 aaggcgttgg ggttacggaa gcctcagggg tttccgttgg cggtgcaggg gtgggcgtct
    26101 ctgttggcgg cggtgtcgtg gcggttggcg tcagcgtgtt caggtaaggc ggagagaact
    26161 gcaactggag caaagcatcc aatccgctta cagcaaccac taccaggcgg tcctcatcca
    26221 cacgcacata atagccgcgg ttcgatgggg tcatatcgcc gatttccaga gtgtgggttt
    26281 ttccgctggc aaaccctacc gtaataacaa aggcgggatt ttccaagccg aaatctgcca
    26341 ggtcaccttg aacagtgctg atctcctgaa gtgcggaaac ttgcgtcgcc gccgcttcag
    26401 acaaccccgg atctgcctcg gtttcgaagg gcagttccag cacccagaca ttttcctcgt
    26461 ttcgcaagac ccgaaccgct tcaccttcgg caggctttac ttcgatgctg gagggagtcc
    26521 cttctgtggc agggaacagg ggtaccgttc ctgaagtggg cgttgcctcc actgcggttt
    26581 cttccttttt gttcaggaag attgcaaagg taaccagccc agcgaacagg acaagggcaa
    26641 tccacgtgcc gatgccaatc acaggcttct gaatttgcgt gccagtggat tcccgtgtaa
    26701 aggttgactc cgtgacaacg ttcgtctttt ttcggcgcgg agatgccttt gccgtgggtt
    26761 ttgtttcttt tcgaggcatg ttctacccct tcttgcggcg tgcgatccag gaggaaacac
    26821 cggagaacac gatcaggcca ggcagcacga agatggaaat gatgatcatg atgatgaaca
    26881 caccattggt gggcggtgtg aacatgcgct gggtctgttc acgcggagta atgttgatca
    26941 ggtcttcctg ttctgcggcc caatccactg aattgatgaa catgttgccg ttgccgtagg
    27001 catcgaagat ctcgtcggtg gcaaagacgg aattcccgaa tacgaccacg cgcccgccgg
    27061 tggcggagtt ttcggccact gcagcaaggt tcagcggacc gggggtatcc ttttcagggc
    27121 tgaattcagg ctgggtctcg gcagacattt cggtttcacc ccacgattgg tcggtcgtca
    27181 aaatgatggg ggtggtggat acgttttcga tcggttcctg tgatacgctg atgctgcgtg
    27241 cctgcggcag gatgacgatg taattctcgg tcaggttttg tgtgatgggg tgatccacga
    27301 tctgtgagga aacagcctgc aacgggttct gggtgtttac gaggtcgatc accatatctt
    27361 cattcagttt gatgccccag tctgagtcga ggtatttggc gagcgggtca aaggtgtcac
    27421 caaattcagt gacgatggtg gggtcttcca tgactacgag ggaaccgcct gcttccatat
    27481 agtccttgag cagaaccatt tcgccgctgg agatcggttt caacggaccc gcaatgatga
    27541 tggcgagggc atcttcagga atcttgttcg tggcgatcag gtttaacggg ctgaccgtgt
    27601 aattctttga ttccaaagtg gattttgcaa tggagaagct cagttggccg ccgcccgctt
    27661 caagagaggg ttcgccatgt ccttgcagga aatacaccgt gcgttcaccg gggttaatga
    27721 ggcggatgag agcctgtgtc aattctgatt cgctggcgaa ggaaacaatt tccttggttt
    27781 cgcccatttg aagcaggatc ttgccgtcgc cggttatgcc agcttcacgg gctgaaacgg
    27841 ggtcctggtc agggtcaatg aatttgtagg tgaacttgcc attgctgttg ttcttaaatt
    27901 tggtgagcag ttcttcagca ctggcgttat cgagattcac gctgtagtag gctgtggcag
    27961 tcacgtcacc gggcagggtt tcaagggcct gcagtgtttc aggggcgagt gtgttggatt
    28021 tatcctcggt catatcccag ggagatttga ggaaatcagg atttctgaag accagcacat
    28081 tggccgtaat gaggatgccg agaaatgcca cgatgaggat cagcgaattg gaaccgtagc
    28141 gggcctggcg tccggtaagg aagcggcgga tctggtcagg gaccatgatg gcgtaggccg
    28201 ccaatccaat gacgattaat gcgaggctga tgttgaaggc caggtcaatg tttttggtga
    28261 gggtttcttc cagggtaaag atttgctgcg ataccatgcc tttggcaatg ccagtgaggc
    28321 ctgaagaaac acaggccagc ccggcgacga tgagcccaat gaaagcatat ttcgcaggag
    28381 agttgttttt attgtcaatc attagcgcca tctccgaatt tcaattgcag tggtaccggc
    28441 aaacaggccc aaagcaatga gactgatgta gtaaacaata tcgccaagag cgatgctgcc
    28501 acggttcatg gatgagaaat gttgcgacag gctcaggtag ttgtaaacat caccgccttg
    28561 ctgcaggtaa ctggcgggca ggttgaccat gaaccacagg aagaagaaca ggccgaaggt
    28621 tacgaagaac gcggcgaact ggtttgtgaa aatggccgag atgcctgtgc caagcgacag
    28681 cagggcggcg ctgatgagga tcaggccgat gtaggaggaa agaatgatca tccaatccac
    28741 gccgggggtg acgtaattat caataatgat cgggaagatc agggtgaaca gggcaatggt
    28801 cagcacgaac atgaaggcgc ccagaaattt ccctgccacg agttcaaaat cgcggacggg
    28861 agcggtgagc agcaattcga gtgtgcccat gcgggcttca tctgaaagca ggcgcatggt
    28921 caatgccggg ctgaggaaga tcagcaaaaa gacgaacagc cagttgatgg gagtgctgtc
    28981 aggggcgggg ccgcccatca tggtttgctg ggcgctgacg aacatgatca atgcaaaata
    29041 aatgccgatg ggcaggacga tggcaaaggc caccacataa gccagcgggc tgttgaagta
    29101 gttatcgtat tcgcgtttgg cgatggtcca gatatttctc atggaatccc tctttaattg
    29161 ttctttgttt cgctgccggt gagctcaagg aagatttctt cgaggctcat accgagtggg
    29221 tgtaattcaa gaaggtcgta accggagttg atcacttgtt tggctacttc ggggcgggca
    29281 tctttgccgg agacaaagtc aaactccacc gaattgtcct tggtcagaac ggcgtgctcc
    29341 acacctttca cagccttgat caggttgacc agattggcat cggcctcgcc gcgaacacgg
    29401 accaccacac gctcaccgcc gatcaaacgc gattgcaggt tttgggtggt gtcttcggtg
    29461 acgatcttgc ccttgttaat gatcaaaaca cggtcgcaga tattttgcgc ttcgtgcagc
    29521 aggtgagttg atagcagaac ggtgcgttcc ttgccgatct cgcggatcaa ctcgcgaact
    29581 tccaccacct gaccggggtc aaggccgatg gtcggttcat cgaggatcag cacctcagga
    29641 cggtggatca acgcctgagc cagacccaca cgttgtcgca ttccttttga gaggttgcca
    29701 atgtagctat cggcacgatc gatcagaccg accatatcca gcacttcttc cacgcggtca
    29761 tctatgttgg ggatcttgcg aagctcgccc atgaacttca aataatcgaa ggcggtcata
    29821 tcggtgtaga gcggaaccgt ttcgggcagg tagccaaccc gcttgcgaac ctccaacgat
    29881 tcctccacca cgttgtaccc ggccacaatg gcttcgccgt ctgtgggggg catgtagcca
    29941 gtcagaatgc gcatggtggt ggttttcccg gcgccgttgg ggccaaggaa gcccacgatc
    30001 tctccctgct gggcatcgaa tttcaggtta ttgatggcgc ggcgtgcgcc gtagtccttg
    30061 gtcagaccac taactttgat cattgcctct ccttgcagta aaaattatgt tttaagaatg
    30121 gggttttgcc aaataaaaag cacgacccgc aggcagtgct atctgttttg ccgttgatta
    30181 ctatacaata ggtagagtcg caaagtcaag caggtaatta acttctaatc taacgctaat
    30241 tttctaggcc ttcggctata atttgcgcat gaccgaactc ctctatcaaa ccgatgcgta
    30301 cctcaaagag ttttcagcca ccattctgtc ggtggattct gaaaaccgtt ccgtaattct
    30361 tgaccgttcc gcgttttatc ccggtggagg cggtcagccc tgcgattttg gcacactgga
    30421 ggtggcgggt gttgtctatc cggtggagaa ggtgaaaaaa cagggcgatg acgtcctgca
    30481 ttttctcggc ggaaccgaac ctttgccgac ttccaattcc gcttcacgct gcgcggggac
    30541 cctggactgg gtgcggcgac atcagttgat gcgcacccat acagcattgc acatcctgtg
    30601 cggcacggtt ttccgtgatt atggcgccaa ggtcaccggc ggcgatatgg atcccctcaa
    30661 gggccgcatg gattttgaat ttgaaaccat gcgtggagag ctggtccgtg atatcgaagc
    30721 agcggtcaat ctcgaggtcc agaatagacg cgatattcgg gtgaagattc tgccgcgtga
    30781 agaagccttc caaatcccag acctgatccg caccaagatc aaccttcttc ccgagggtat
    30841 tttgcaggtt cgaacggtgg agatcgtcgg ccttgacctg caggcggatg gcggcacaca
    30901 tgtggcgaat actgctgaag tgggccgcgt aaaagttgtg gattacaaga gcaaaggcgc
    30961 gatcaacaaa cggctttata tcgaaatcga atagacccaa taggggaata ctcgttgccg
    31021 ctcttgtttt ataatgccag aaattaacca aaggagaatg tttaaaatga aactcaaaaa
    31081 tctctcgctt ttgcttgttg tagctgtgtt gtttgcgtct gttttggctt gtgggtctac
    31141 cgcccctgtg ggcgtttcca atgtgtacat ggcaaatgat gaagaaggca ataacaagac
    31201 caccactttt gcgccaaccg atgtcatcta tgtgtttttc gatgtgaacg gcattgacga
    31261 aggcgcacag tttcaggtga agtggtatgc gttgactgtg gatggtcagg atccgaatga
    31321 agcgttcacg accactgatt acacctatac tgatgaagcc accatctatg ccaatatcga
    31381 aagcacggat ggcggtttcc ccgccgcaca atacaaggtc gaaatttacc tgaacggcgc
    31441 gaaggtgggt gaacagcagt tctcggttca atagtttttg aagccgactt ttgaagatgg
    31501 cgcacctcga tacgatcgag gtgcgcttta tttttaagaa ggtttgccgc ttacaaaaaa
    31561 gcagggaaaa gaagaggtca ctcccttatt gatatggcag ccccgggtgt actgcatcgt
    31621 cgtataattc acaataatgg aggcaaacat ggagtatagg aaatttggca aaacgggtct
    31681 caaggtctct cctttgtgca tggggaccat gcaatttggc tggtcgctcg gtgaggcaga
    31741 atcgcacaaa attctttcgg ccacttttga ggcggggatc aatttcttcg acacagcaga
    31801 tatttattcc aaatgggtgg atggaaaccc gggtggtatt tctgaggcgt acatcggcac
    31861 atggatgaag caaaacaaga ttccgcgtga ccagatcgta attgccacaa aagtacgtgg
    31921 tgagatggga aatggcccca ataatgaagg actgagcagg gttcatatta tgaatgccgt
    31981 agaggggtcg ttgcgccgtt tgcagacgga ctatattgac gtgtaccaat cacactggac
    32041 ggatgaatcg actccgatcg aagagaccat gcaggctttt gatgaccttg tacggcaggg
    32101 aaaagtccgt tatgttgggg cctcgaatta tgcagcctgg gagttgatgc aggctctgtg
    32161 ggtttcagat aaactcaatc tggctcgata cgacagcctt caacctcact acaatctgat
    32221 ctatcgcgat gagtttgagc gggaactgcg ggatgtctgc aaaacctatg agatcgccgt
    32281 aatcccatac agccccctgg caggcggctt tttgacgggc aaatatcgcc gcaatgagcc
    32341 tttgcccgaa agcaagcggg ccgaaggccg caaacgggca atgaccgaga aaaatttttc
    32401 cctgttgtat gaaatggaat cgatcggttt gcgccacaag gcctcgattg cacaggtgtc
    32461 actggcatgg atgctttccg atgcggtgat caccagcccc atcattgggg cgaccaccat
    32521 tgaacagttg caggaaaacc tcggggcatt gaatgtccac ttgatggatg atgaagttgc
    32581 taccctgaac aaactaactg cctggaaaac agacgaagat taatccctga attgtttttt
    32641 gtaatataaa acatgacgca tcctcgaatg gttcgggatg cgtcatgttt ttaaatgtga
    32701 tactgaaaat attttttacg aaagaatcac atgcccgcag tactcgcatt tgtccatgcc
    32761 gagttcaagg gtgccgccgc agtgggggca ttggaacgtt tcaactttaa tggtgtaacc
    32821 tgctgtcttg taggctgttt cattctcagc caagtcgctg cgcagacgtt tcaaccttgc
    32881 gaggtaggca tcacctgtca cttcgcctgc cttgcgcaaa cgttccacat ccgcaatgga
    32941 ttctttcaac atgcggcgct gggcttcgag agattccttg cttttatcct ggaaggagac
    33001 cttcaattcg ggtttcggcg gaatggaggc gaggaccccg gcaatgacca gcatgatcgc
    33061 tgaaacgacc agcccgacga tcagcggggt gaactggaac ccggggatgg agttgaaatt
    33121 gaccttgttg gtttggacat gactgccatc tgcgatcact acataccgtt cattgcccag
    33181 cttgccgatg tcaatgcggg tgatgccggt gttatgagag ggcaggttgt tgcgtgtgcc
    33241 attttcggtg aaaacagcca ccttgcctga atcgtcaccg atgacggctt cttctttgcc
    33301 gtcttcattg atgtcgaggc ctgaaatttc ggtgaccttg tccgacaggg aggcggtcca
    33361 catttgagtg gcggttgtgc cgttatagga aaatgcccaa atgccgccgt ctttaccacc
    33421 gacaatgatc tcgcgtgagg atgggtcgcc atttagttcc acttcacgta cttcgctgac
    33481 cgcctgtccg agtgatttgc tgaagatggt tgagccatcc acggcactgt aaatgtagaa
    33541 ggcgcctaac tcgccgccag tgatgatttc gctgttgcca tcggcgttca aatcgaaggc
    33601 acgcatgcgg cgaagctgtt cctgttccaa ctgccaaacg gctgatccgt ttgcagcgaa
    33661 gacaccaact gtcccgtcgt gcgaagcgat ggcggcatag gtctggccgc cgatctttgc
    33721 gtcatccatg ccgcggattt cctcactcgc aaagtcagat tcccacacga tctttccatc
    33781 ggtggtgatg gaggcgatct gcccggagac atctcctaaa acgatctggg agccgccctc
    33841 aaaacggatc aatgccacgc gtgcagggac aaaaacaggc aattcgctgg caatggtctt
    33901 ttgctggccg ttgataatgg tgtctaccga aaaaaagttt ccgctgtgat aaacgacaat
    33961 atcctccacg ccatcaccgt taacatcgcc caatgtggtt ttgggtttat cgtaggtgaa
    34021 gtcactgatg acattaccgt tggggtcgta aagccttatg ccaaaggcgt tttgaatgaa
    34081 aagctcatcc tgaccatccc cggtcaggtc gatgattttc atgctttcag cggttgggta
    34141 ttcgctggac cagaccgggt cactgccgaa tttttccgcg gtcactgcgc cgctgaaagc
    34201 gaacacgccc gctacaacca tgacaagaca ggagagaacc aatgcaatga taaagggtat
    34261 gattttgcga ctcatttatg cagccttccg ttgtaaaaga gaataagttg attttaaaat
    34321 ggcaaggatc tgttcgttgt ccacttcatc aaagggggag ctggcaacca tgttgatcat
    34381 gccgccgtct gtgcttaatt gagaaatggt gtacttgccg atgttcacgc ccgggcgaaa
    34441 ctccttgttg gggacgatct ggtacgcttc cgggttgacc acaatacgta ccttcagatc
    34501 atgtgatgtg cctttatatt tttcgggctt taactttgat tttccgctga ttcggctgcg
    34561 cttccaatag gaggcgcgct ttttggtttt ttgtatcgcc gaaatgcgca gaacgttgcc
    34621 gtcatatagt tttgctttca acgaaagcca ttcatcgcgg aaatgatgag tggtgcggtt
    34681 ctgcgtatca tgcgtttcgc gggccacctt gtttttcagc attgcccctg tcaggtcgag
    34741 atgaccaaga aaggtgctgc cgggtttcaa gtcgtcacgc agggtgtata aaatgtcctt
    34801 tgtgccttta taacgcggcg gaaaatcgag cgtatcgctt ctgcgcaaac tgcgcgccat
    34861 gatgaacgcg gcgatccaca acaataaccc gcctgaagtg aaaaccagcg aaaaataccc
    34921 aaacagcaga agcacaccgt ccacagccat catgccgagg ccgacgaaga aaatcataag
    34981 gggaatccaa cgccacttgt caccagcggt ttcggtcttt tgcacctcgg ctttaaatcg
    35041 ctccatgccg cgcatgatcg aatcgggctt gtcggcaatg gacacataga caggggcgta
    35101 ctcttttttc cctgcaatgt tggtgggggc cttaaccttt tttcgggctg tattcaatag
    35161 ataatagaag agcgacacga caacaaggcc gactacgcct aaaacacata acgctggcat
    35221 gaaacattcc tccgagtggc ttacaggata ttgctaaaaa ttcttttttg ctgaaagcga
    35281 tataatttca gcaatggcca agattctcta caccgctttc gatattgtac caagcccgaa
    35341 aggcgcatcc acgcatattt tgcacaattt gcgcgggctg gtcaatgggg ggcacagtgc
    35401 ccatcttatc acgcccaacg atgggcttct acccaccgaa gatactattg agggcgcgca
    35461 gctcacccgc atttcgcagg acctctcgca gaacttcctt gcccgggcca tgcactttgg
    35521 caggtcggtt gcggcacatc tggcactgca tcaggggtac gatgttgtcc attttcgaaa
    35581 tatatgggat ggcctctcca ttgcgcaaaa taaaaaaagg cttggctaca aaacactttt
    35641 tgaagtgaat ggcctgccct ccatcgagct caaatatcac taccccggac tcgaccctga
    35701 attgctcttg aaaataaaag aacaggaaat tgcgactctg cacctgagcg acgcgatcat
    35761 ctgtccttcg aatgtgactc gcgattacat cgcctcgctc gggcttgacc gtaaactggt
    35821 cactgtcatc ccaaacggag tcagcccttc ggacttttcc ccgtcatccc tgcctgcccg
    35881 cgatggaagg attcccgtgc tgctttatat cggtactctt gccgattggc agggattgga
    35941 tatcgtcgta aaagcgctgc caaagatcct cgaaaaacaa cctgttcact taaaaatcgt
    36001 cgggcgcgga cgttcccgcc agcgcaaggt gcttgcgaag caaattagaa aacttggcat
    36061 cgaagaacat gtaaccgtac agcctgccgt accgcaccat gaaatccctg cgcttattgc
    36121 cagtgcagat atctgtgttg ccccgctcgg tttgaatgac agaaatgtca cccagggggc
    36181 gtgcccgatc aaagtccttg agtatatggc agcggaacgt ccgttattgg cttcgaatat
    36241 gcccattgtg cgtgagcttg tccgcgaaga tatggatgcg ttgttatttt ctccaagcga
    36301 tcctgaagac cttgccagac agaccatcac cttgctgaat gattttgaat tatccaaacg
    36361 cctggcccga tctgcctccg aacgtgcgtt gaccaaattc acctggcacg aatcgcagaa
    36421 gaaattattg aaggtgtacg agaagttgct ggcttaacca gcaaaagacc caaacaagga
    36481 cgtgcattga ttcactttgc ctgatccgcg ttgcgccacc aataaaatgt gatgaaaaaa
    36541 ccgactgcgc ttgcaatcgc cacaatgaga tagctatgca ttccaagtgt ttcagaatgt
    36601 gctccgatcg ctccgctgaa cagggcgggc accgaccacg gaagccagtc accccatccc
    36661 attacgacag cgacctgtgc aatggcaacg gtaaagatcg cccagccaaa agcggccata
    36721 aatccgcgcc cgatgctggc gatgaaagct acaaacggta acagcaggat cgtcaggatg
    36781 cccgcgccaa gaatatcgat ggttgccgag cggaagagtt catttgtcca gccagggatg
    36841 gcaacagcat tcccaatgat caaaccaagc ccgtaactaa gcagggttag ggccaccgtc
    36901 caaaaaccga tcacagtgaa ttttgccgcg atgattgcct cgcgcgatgt gggcaatgag
    36961 agcaactcct ttacagtatg atctgaaaat tcacggccaa acacccaggt ggtgacaatt
    37021 gcaaaaatga tcgcgccgcc aatggccgtt gcttgtgcaa gcagattgaa aaatgtcggc
    37081 cagtctgcca cacctgcgga caattgtgct tttgtgccga tcaaccccat ggattttgcg
    37141 gcttcgggat ctttcaatat gaccatgaac aggccgccga ttaagggcgc gatcaaaaat
    37201 cccaacaggg tgaatagcgg cactttcgaa cgaaatgcct tgagactctc ggtttgtaaa
    37261 gcagcaagat aagcattcat tttgtacttc ctgtcaattg cataaaatgt tcttcaaggt
    37321 tttgctgttc cactgcaaga tgggtgggtg gcgtgtcagc attgaccagc aagcatgcga
    37381 ttttctccgg tgtttcgagt gcccgcgtat cgctgacgaa aatagaatct tcagtgatcg
    37441 ttgccgaata gttttccctg gtcagcactg catgcgctct tgcaaaatca cggaccttga
    37501 tcttcaagcg tctcgcgcgg aattgctcaa gtttgttcgc gtcgaggtct tcgatcattt
    37561 tccctttatg gataattccg attcgtgtgg cgagcctatc cacttcagtg aggatatggc
    37621 tggacatgaa aacggtcacg ccgtttttcg ccagattggc gatcaactcg cggatttcaa
    37681 ccacgccagc agggtcaagc ccattggcgg gttcatcaag gatcagcaga ctcggatgat
    37741 ggatcattgc gcgtgccagc ccaaggcgtt gaaggttccc caatgaaagc gcaccagctt
    37801 tccgtttgga ataagaagcg atggaaagac tttccatcac atcatccact gcgttggaat
    37861 ttttcaatcc atgcaatctg cgggcgatat ccagattttc gcggacggtc agatcggggt
    37921 aagcagatgg actttccacg agatgcccca cctgcgccca tggtccgctt ctacctggac
    37981 ctaaaacctg acccagcact tttacatttc cttcacttgg gcgaatcatc cccaaaagca
    38041 tgcggatggt ggtggttttg cctgcaccgt tgaggccaag gaagccatat atctcgcctt
    38101 gcttaacccg taaattgacg ctgtttacag cgtttatgct gccatatgtc ttggataaat
    38161 tatttgtctc gattacgtat tccattttat tctccagcaa tatacccagc caccagatca
    38221 atcagcacac caatggtttc ctgataggct gtttcaccca tatcgtcacg atgcagcccc
    38281 ataaagaaca gactcttgat gaggttgctt acaacccgcg gaggcgcctt gacgttaatg
    38341 ccttcgcgtt tgatcttttt tatgaactcg gttgtgaatg cctcgtcgtt gctggcgtgt
    38401 tgcatcaccc gttcggatgg aattttcctg acaaggtaat caaaatcggt tttgctgaaa
    38461 ttcttcagca gggggtaggc atcccatgtc aacaatagtc ccttgagcat ctcgctgaca
    38521 tttttctgcg cgtttgcttt tggcgcgatc gtgaactcaa gaatttgggt ctgaatttca
    38581 ttttcgaatt gttccagaat ttcaaggaac aattcctctt ttgactcata aaatagataa
    38641 aaggcgcctt ttgaaatgcc cacggcttcg gtcaattcat ccacactggt ctttttgagg
    38701 ccgtgtttct caaagagctt ttttcccttt tcccgcatct gcaagcggat ggtgtctttt
    38761 tcttgttctg aaaatgcttt tggcatggga ttgcctttct gtgactaatt tagtaaaaat
    38821 ggtcacagaa attatattca aaaccgaaaa ggagtcaagg tgaggtgaat aaaagaaagg
    38881 cgtgcaataa atttgcacgc cttgaaaaat agatgttttt tgtattgctg attattcggt
    38941 tcttccgagt aattccttta cccgttggga tagttcatca atatgaaatg gctttgtgag
    39001 gtagtcgtca gctcctgcgc caaaagcgac cactttgctg tcgctgtcat ccagtgctgt
    39061 gacaatgatg atgggggtaa agatgaatcg cggtgtctga cggagcattc tacatacttt
    39121 gaatccgtca gggtctggca tcatcaggtc gagaataatc aagtgtgggt ttactgaatc
    39181 ggcgatctgc acggctttgg aactgtcatt cactgctgtt gtctcgtatc cctccacggt
    39241 tagaaccttt tgcaacaggt ccgttgcctg tttatcgtca tctacaatca aaattttggt
    39301 catgacattt tcctgactga tttggatgaa ttttcctgaa taatattttt aatattatac
    39361 cttagacata tttaaccgaa agaccgcccc tgcgatgaaa attggcagtt gcggtcttcc
    39421 tcgcaaattt gtaagctgtt gcttcaggtc agtgcgttta ttttttgggc taattccttg
    39481 ggcaggaagg gttttgtaat aaagtctttg gcccctgcgt taaatgcgtc ctttttgctg
    39541 ccaatatcgt tcaacgcaga gacaatgatg atagggatgt gtttgagggt ctgggtcgac
    39601 tggaacattt tgcacaactg gatgccattg attctgggca tcataatatc caaaagaatt
    39661 agatcgggaa tgatggaatt ggcagtttca attgcatcgg cactgttatt gatcgatgtg
    39721 ggcgagtgcc cgttcatttt taccaggctt tccaaaagct ttgtggtttc catatcatca
    39781 tcgattatta atatttttga catttcattc ttccttgtga ttggagtttt tcagcagatc
    39841 gaattccaca ttgcccgaaa agcttttaaa aggacttatg aatacctgta aaataacgat
    39901 aataatacaa ataccacttg caattattgt gtcaaatctc agccattcaa cagggttgat
    39961 atcctgaggg aaagttcatc aagattaaat ggtttcgcca gataatcatt cgcgccaaat
    40021 gaagttgcct tgctgttgct atcggccatg gcggtgatga tcaaaatagg tgtcagtctg
    40081 aaagctggat cagctctcag catactgcaa agcgtgaaac catctggagg cggcatcata
    40141 agatcaagga tgaacagatc tggatgctcg gtatttgcca gaggaactgc cttggcactg
    40201 tgagtggtgg caatcacttc atagccttcc actgaaaggt atctttttaa tagcgttatg
    40261 acctgcgggt catcatctac gatcattatt ttgggcatga tatcttccca gtatggtgaa
    40321 atttctcctc aatcatacaa ttaaaaagtt tacggcgcaa gccatagagg tgagaccatt
    40381 aaaacctgat ttcttctgcc tgcagaaaat tggcatgaaa tcgaaattaa tcggccgtaa
    40441 ctttttcaca gggaccgttt cctggcaatg gcagcactaa tctgcctggc ctccattgcg
    40501 atctcgcgga gcattccagt gggtgataca taatacccgc aaaaatacag gcctgtcaca
    40561 ccagcctcac gccctgtgga gatgggcgtg ccattttcgt catataccga ttgttcctgc
    40621 aaaaaggagt tgaccctggg ttgatagcct gtagccagaa tgatggaatc aaaatccgct
    40681 tcttttccat ccgtaaattt gattctgttt cctgcgaaat tctccacccc tgaataaacg
    40741 tcgatgtgcc ctgcgcgaat caatttcatg gtgccgatat cgatcaaggg aatgtgtttg
    40801 tcgtttctga tctgggtggc ggggccatag ggcagtttgc gcaagccata ttgggtgata
    40861 tccccgatga cggcccgcaa gatcggtgca ttgattgcat cggcaagcca gacaggcatc
    40921 aaattttgca cgatgccgat ggagagaatg ggaattccag ccaattcctt ggggataacg
    40981 ttcactgcgt ttcgaactgc aatccctgcc ctggcgccat gctcgtacag gtcaatggcg
    41041 atttctccac ccgagttgcc aaacccaatg acgaggaccc tctgcccttt aaattcagat
    41101 ccgtttttat attccgaact gtggatgatc tttcccttga aaccatcttt tccgttccaa
    41161 tcagggatgt atggttcacg cgcataaccg gtggcgataa ccagttgtga tgcgtggtat
    41221 tgtgcattgt ttgtctggat ttcccatctg tcgttaattt tcctggccga tgtcacctgc
    41281 tggttgaaga gaatattcag gtcaaatttt tttgcgtagg cttcaagata atccacaagt
    41341 tgatcgcgtg agaggtagcg gggatattcc tttgggaatg gcgcaaatgg cagttcagaa
    41401 ttctttttat cggtgtgcaa atgcagtcgg tcgtaatgac gcctccagac cgggcccacc
    41461 gcgttatctt gttcaagaat aatggctgaa tggttggcct gtttgaggca ggctccaacc
    41521 gccagccctg cagggccagc gccgatgatg atggtatcga tgttttgatt ggacatgcat
    41581 ttaagtgtac cgcagacccc ctgcttttca aactccagcc ttatggaact ttttctggcg
    41641 ggcttgtttt tgcgccttcc caaaagcgga tgacctcttt ttctgcgtcg gtgaagccca
    41701 gccagcgcac gacatcctgt ttattattga aaacgccgaa gttggacggt gcttccggtg
    41761 acatgagttt gaaggcatcg gccaggaaca tcaggcttgt gctttttgtg aaaacggcgg
    41821 tttgcccaag tttttggcct ttttgtttga gctgcctgtt tttttctatg gccaggtgca
    41881 tatccgaaag ggaggtttcg atttcagccg aaaagatatc gatgatgatc ttcatgtttg
    41941 aacagtagcg cggatcttcg gggaccttat cggcggtctt gaagaaagca gttccggtcc
    42001 aggaatctgt gcagatataa actaaaaggt tgaaggtgtt tgaaatatcg taggagattg
    42061 gcattttgaa atacatccag attaattcta aattcaagta ttcaaaatgt cattcgtatt
    42121 acccgcaaat ttgtcaacgt attttctcgt aaatctcact acgtagtttg gcggagagtg
    42181 gactgaaaaa cttcgattta attaagtagc cattcaagcg cgctttctgt atcttgaaaa
    42241 attcgggctt tgatcattcg attattggtg actgtttcga aaaaacggag atcgctggat
    42301 acgcgttgga tgtttgacgg ttcaatcagg gcttccttga tatgaatgcg caactcgagg
    42361 ctgatgcttt caagaaattt tcccaactcg taaatatcca tggtgcttcc ttgttgagtg
    42421 aaactggaac aatcgccaag gaataacccc gtttgctttt cggcagccat ggagaaattt
    42481 gccattgcac attcttttaa ctcatccagt gtgatgggtt cggtgtagat cgtttttatg
    42541 atcttggtgt cttcaagata ggagattttc cagggcatgc aggtggtcct tttggaggac
    42601 ctgaaaccca gatcctccaa tttgagattt tttgtttatt ggatttggag gatatcaaat
    42661 ccttcgaaga tgcgttttag ctgggcgagg acatttccgt aagtctcgtc atccacttga
    42721 aagtcaacct ggatctcgcg aaaatcctct tcaccgaatt cttgtacctt ttccggcgac
    42781 acacggacta caaggctgtt tcttgagacc tgcacagttt ccagaccgcc atagcttcca
    42841 taggactggt cgttgatttc gaagtagtaa gagctgtttt caaagcccgg ttcgatggga
    42901 cgtgagaaag agagaacgga aggttggtcg gggtcggtct ctgggtcggc aaacatgaca
    42961 tttgcggtga cagtgttggc atacggttcg atataaatgg cggtgaattt catgtgatca
    43021 tctcctaaaa ttttttacct gcttgttttt ttcagtgctg tttggacggt cattttgttt
    43081 tccacaaaaa ctatcccgtc attaattcat ccaccgcctg tgccagttcc cgtaaatttc
    43141 cgacctttaa aacattgctg cacagggggg cgtatttcgc catatcgctg tcaccgtgcc
    43201 acatggctgg tggctcagga tttagccaga tggtgcgtgt agcgcggcgg gtcattgtgg
    43261 agaaaatgtc aatgcgtgga tcgttgtagt tattgcggcc gtcgccgacg atgatgaggg
    43321 ttgttccact gttcaacgtg tccatgtgtt cactgttgaa attgtccaac gcccagccga
    43381 ggtcggtgtt gtagtgaccg ctgggcatgc gccacagaac agattgaatg gcttcgtcgg
    43441 cgttcgcccc gttgaaatcc tctgaaatgg attcgagatg gtcaatgaat gcaaaggcat
    43501 gtgttttgcg tacctgtccc tgcagtgcaa aaaggaaact gagcatcagt tcggagcaaa
    43561 aacgcatgga tgtgcttaca tcgcagatca ccacgatctt tggcttgcga accttgtcac
    43621 ggtgtttgat ctccattggc acaccgtgat atttcaaatt tgcgcggatg gtggcttttg
    43681 ggtccatttg cccgcttttc atcctttttt gccgcaaggc aatgcgggta cgcagcgccg
    43741 ctgccagccg tttcacttca cgttgtagaa tcttcttgtc agcatcggac aacgcctgaa
    43801 atggacggtt cattaagttg tcgatatttt caccgcgcgg cttttcgctc aggttttctg
    43861 cgatgcgctg gcctgcaaat tgctcgatct ggttttgcat cccctgcata ttttgttgaa
    43921 ccatgttgcg gatttgttca atgcgctcgc ggttcattcc catttcctga agctgttcca
    43981 tcagctcgcg cattgctttc tggatctcag gaaaggccat cgcccgcatc atccgttgcg
    44041 ccatccaatt ctgatagttg aggtcgccag cttgattcag ccccacaagc tgacccaacg
    44101 cttccaattc agactttgtc agcggttcgc cattcatcag cctttccatg ttttggcgga
    44161 gactttcttt aaactgacgc agtgcttcgg ccagcatttg aatttcttca ggtgaaagat
    44221 cgtcggttgg atttccgccc atcattggcg gctgtccgct gccaaaaaac agcgggaaca
    44281 atttatcgaa ggtgggaata tccttgacat ctttgatgag cgtggcgcgc aatgacaatc
    44341 gaaatgcttc gcgttcctga atacccatca aatccacagc tgaaaaagct tcagctgatt
    44401 ccgccaaaga aacgcgcaca ccgctggcgc gcaaggctgc gattagttgt aagattcggg
    44461 attccataat tcaagcactc caattcatca gacgtatttc aatgcattaa ttcttttttc
    44521 aactcttcat acgaattcaa gccgatcttt ggcataagct cactttccca aatggcatcc
    44581 aattcctgct tgatgttttc aggcaccgat agtttcgcac ctccgacctg cccatcccgc
    44641 accttggaaa gctggccgtc catgggcagg cgcatggctg cactgcgcga tttgcggatg
    44701 atgtggtctt caaatttatc ttcatgtgcc tgcatgaatt tgatgtcagc ctgccttaca
    44761 acaatctctt tcaattcgtc atcaagtgtg atgccgatga attccgcaac ccgttccacg
    44821 gtccgcggta gatccgcttt catattttca aagcatagcg gcagaacatc tttatctttc
    44881 cgtcgttccc ataatgccgc aatgtgtttg aaaacggccc gcccgggcag gtaatattcc
    44941 tttgcaaagg tctcaatgtc aatggagcct ttttcaaaaa agaaaccttc aaagaaataa
    45001 taatgagaaa gcagggcatc cctgggttcg cgcagtacca caatgtattt gccgccttgc
    45061 ggcacttcat ccatggtgct gtgcgtttta aatgcgcgcg gatgagcgac ctgcggcgca
    45121 tcaatatcaa tgcctgaatc atgtgccaag ttgatccacg ggatgacgcg tgtgatctca
    45181 tcgaaatcca tcgagccccg tgatctgagt ccatgaacga tctgctgcat ccaggtggtt
    45241 ccgcattttg gaggtgttac gatgaacaca tcggttgggg caggtttata ggcgaggcct
    45301 tttttccagc cttctggtgt tgcaaaattt ttcatccgct cttgcatttc tgcgatcgtg
    45361 gtcggacggg tgaacgtttc ctgggttgtc atctttttac ctctaaagtg ggatttgttt
    45421 tcaaacgatc aaggctggtt atcgttttga atttcttgaa tcaaactggt ttgttctgtt
    45481 tccgctgaaa aaataaattt atcagttacg tacactccat gtggacattg accgcttcgc
    45541 accgctgccg aaacctgccc agccaaataa acagaatcag cgctttgacc ttgatccagc
    45601 ggacccagcc tgcacgattt tgcagcaggg tatttctctt tgttctccac aagatactcg
    45661 attgcctcaa gattggcaac gatacgcggc aggtttgcag ggttgaactt tcgcgcataa
    45721 ggcggcttga gaaaatcggg gcgtgtgatg tagtcaatgt tgaaaatggc ctgcactttc
    45781 cttccgtctg ggtcggtgta cccatatagg gcggtttgtg aatcttccca gcggggagcc
    45841 ctgccaccgg ggagagccac catatttcgc agttttggaa tcaggtccgc gcgatgaacg
    45901 agttgtagca gttcgcgcag ggtttcgtca ttccagcgaa gttctgcggt cactccaagt
    45961 ccttttgtgt ttttcagatt tacatgccga aacgtgtggt tgtagatcat tgcatcatgt
    46021 tcgatgcacc acacgatcac attcgccagt tgtatctttt gttcaatgct ggttccctct
    46081 ccgtagggtt tatctcccag gttggagaac agagcccaat gaaacccgaa ctcaggatgc
    46141 tttttgtaga actcccatgc cacgccaagg cctgtatctt tggcgggagt cccttcagcg
    46201 gtcaatgcga tctggtttct gaagaagaca tcatccatgc taaagatgac cgggcgcttc
    46261 ccgacaggca cacgaatgtc acctgccagc catttttcaa gcgataccga cacatagcca
    46321 ctgcgataca ggctttctaa ttcctcttca aaatccagcc tgctgactgt gctataagtg
    46381 gatgggcgat tggtcttgaa cttgtgatag gtaagaatgg ctgctgcagg ttggctttcc
    46441 caaaccgtgg cggtgatgta atacggaatg ggggtgggaa gcgctgtacg ggtgggcgtc
    46501 ggcgatggcg gcacagtgac cgttgccgtg gggatgttct gtggaacggt tacaggctct
    46561 gccgatggga cgcccagcga aagggtaagg atggcaaata gagagatcag aatgaattgc
    46621 tttggcggca tggggcactc tatgcaccag cgcttctttt acatcatttg gaaatttcct
    46681 tttttctctt gtgttgtttc cattcctgga aggccatgcc caaatcggga ttttctttca
    46741 agaataacag agacaacaaa aatgcgatta atagaaatat ggtttttaga agcgcggcaa
    46801 gcccattatg gccgtctttt ggcggattct tcattgtagt atggttacta tcagtttccg
    46861 ctgaacctgc caaaatcatc
//
LOCUS       DAOQJC010000012        46506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_328_length_46506_cov_9.690125, whole genome shotgun sequence.
ACCESSION   DAOQJC010000012 DAOQJC010000000
VERSION     DAOQJC010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 46506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46506
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_328_length_46506_cov_9.690125"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1159)
                     /locus_tag="PLE39_03035"
     CDS             complement(122..1159)
                     /locus_tag="PLE39_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNE67325.1"
                     /translation="MWKKIFLCSVLILASCVSISVDSNAQPTAQPDFVTSTLMPTQAG
                     YVPPTLTPVPEATITPTRIPVTAPANCTDSAVLVRDVTIPDNTQVKAGEKFTKTWEFI
                     NNGTCPWIGYTLKFSSGDKMNAPLSAPIPDTLAKERVEASVELTAPASNGTFTGYFTL
                     NDANGNALPIGTEKTFWVKIIVGSGAIVPTTSGGNPTQAVGITPVSNGGGANCSFSQN
                     AGYVNQIASLINAERANAGLPQLTINAELSSAAQGHAADMACNSSISHTGSDGSSAYG
                     RVLASGYSGQFYEEIIYGGGGPEAAMSWWMSDKIHRDAILNPNTTEMGVGYAYFSNGA
                     YGDYITVDFGG"
     gene            complement(1176..3407)
                     /locus_tag="PLE39_03040"
     CDS             complement(1176..3407)
                     /locus_tag="PLE39_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67326.1"
                     /translation="MDFQPYIPGMKPADPGPLSRFLPPLEEGVISGWLSTQPFGAAQS
                     GPGTWLLDPFGFSPRLVLEAARSGTRVLVTANNPITRFLLEMFANPPPESEFIAALAD
                     LGAVKKGDERLAGHLQSLYLTKCEKCNQQIHANAFLWRKGEDAPYARIYECKHCGDSG
                     EHPATEEDIERAKKIAETDTLHRTRAFEKVVALNDEDRFYAEEAIQHYLPRPLYVITT
                     IVNRLDSLNLSAERKRALTALVLIACDAGNTIWAHPAERPRPKQLSTPSQFREHNVWM
                     VLERGLTLWSETGSTVPCEAWPKKIPESGGICIYEGRLKDLAQLVKKEIPISAVIGSV
                     PRPNQAFWTLSALWAGWLWGKEAVEPYRVALRRRRYDWAWNATALHSAFNHLNDLLPD
                     GVPFFAFLPEPEPSFLTSAFTAASASGFSLVNVALRTEHDPVQVIWKSGKKEKPQTAD
                     DSLLQTSIQEYLASRGEPASYLHVHTAGLIALAEANALKQPEHEFDEALRKTNALFES
                     ALKTDERFVHYSTGENVETGTWSLHFGGGAQAKSESLSDQVEVAIVTFLQKNQHAIYL
                     EVEDELYPRFTGLLTPSKGLIYAVLNSYAEKESGGWKLRAEDLASARRSELNDIFALI
                     EAIGRRLEYKTNRTDKTLTWLENGKPIRSFYVMASALFGRALETTAPDTILVIPGGRA
                     ALAAYKLQRDPALAAQLKTRRVVKYRLLRAILEVPILTRDSFEEQIASDPVEKSAGQM
                     MMF"
     gene            complement(3427..4392)
                     /locus_tag="PLE39_03045"
     CDS             complement(3427..4392)
                     /locus_tag="PLE39_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE67327.1"
                     /translation="MDFSTNGIVTVIPAYRVQDTITTVLKDLPAYIKHIIVVDDASPD
                     SSVKLVTAAAQQDPRIILIRHERNQGVGGAMISGFHKALELGAKIVVKLDGDGQMDSA
                     HIPALVTPLILGKADFTKGNRFRDFVSLRNMPMVRRVGNLGLSFLTKAATGYWNIFDP
                     TNGFFAIRTEVLKQLPLEAIDKRYFFETSLLANLYLVNAFVMDVPMPARYGNETSHLS
                     IRRSLLEFPLKLFSTLIRRILLKYYIYDFSIISLYIVTSIPLLLFGFIFGAIKWIDYA
                     SRNIPAPTGTVMLPTLCVILGIQIMLSAIEIDMKSTPSQPICDPL"
     gene            4442..5593
                     /locus_tag="PLE39_03050"
     CDS             4442..5593
                     /locus_tag="PLE39_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE67328.1"
                     /translation="MATGERILLVESDPDIVDLIVRQALQPLGYRVGTAGDAASAIKL
                     SMQTPPDLILANLNLPGLSGKDMLAALSAQGVKSPVIVIAEKGQETDAIQAFRLGAAD
                     VIFWPVRDAEVVSIVERALRQTQQSRERQKLDRQLSAMNTELQRRLRDLTTLLSTAKA
                     VISITDQRLLFDRILESAMQVAEADYCWLTLREEKGNAYLLRAHRNLPEAWAKKMNQP
                     LDDGISSLVGFSGESLVMNGSPLQKFKIAALGKSVGVIPIKIQGEVIGLLMVARKIDR
                     EIARDSQTMLEAMADFASISLVNARLFRALEQSAETARMNEKQRHAALDLVRTNIRNE
                     IQSAGYPLDLVLTETPGPLTPEQRKALEQVQAALQRLASSSEKTTIPNK"
     gene            5606..7054
                     /locus_tag="PLE39_03055"
     CDS             5606..7054
                     /locus_tag="PLE39_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE67329.1"
                     /translation="MSKQELILLAMDASPVLGLMERTLRSAGFDVAIAHDLVGLNKCL
                     HEAIPSLMIVGEKFVDQGGFAIAKEILERFPTLPIIFYAEQDTTGYAKTALKMGLSGY
                     LYPPLKVSDITDEVHRSLERSSKLGDWLRREVRRTTSSLTERAKLSESERSKLDAIIA
                     NIQDGVIVLDDRKFILLANHAIHDIFNFHDREIVGRPLADAIKNADLTALLARAGDSP
                     LKYHEINFDDGRVFNAQYTPIPKIGGAITMQDISYLKELDRLKSDFIHTVSHDLRSPL
                     TSVLGYTELIERTGPLNANQLEFLHRLQGSIQHITTLINDLLDLGRLEAGFDTRREVV
                     QLSGVLHYSLDMFDGQLKKKNITLVTDVAPDLHPLRANPIRIRQMLDNLIGNAIKYTP
                     DGGRVTINMFMQDQQVVLKVEDTGYGIPPEEQSRVFEKFYRATNAIEDIEGSGLGLAI
                     VKSIVDSHQGRVWVESVVGKGSAFIVLLPAHE"
     gene            complement(7244..7660)
                     /locus_tag="PLE39_03060"
     CDS             complement(7244..7660)
                     /locus_tag="PLE39_03060"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HNE67330.1"
                     /translation="MNIYFACSITGGREFETAYQEIVAALEKDGHEIPTSHLVQSEIA
                     EKERQLTPQDVYERDVNWIRNCDTLIAEVSTPSHGVGYEIAFALGLGKPVLCIYQKDR
                     RVSKMLSGNPDPKITTQAYGTVEEAISQARFFLDSL"
     gene            complement(7657..8277)
                     /locus_tag="PLE39_03065"
     CDS             complement(7657..8277)
                     /locus_tag="PLE39_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67331.1"
                     /translation="MAKEKGGRRYPLVIYTHMIKRWWTAVFTLGLALLGLAWAVYSWG
                     MDQWRWLALASIGGLHVFIGLGMWLMRKSAYVQPFREYLKLVTPFLRLNISYRRVRRA
                     TSANMGTLFPPNSVSSWRAEIIQPLAKMTAIVIEMNSHPMPQSQLRLFLSPLFFKDKS
                     PHIVILVDDWMKFSSEMDSLRTGTGPISAQQKRNASILSRLPNKDK"
     gene            complement(8297..8944)
                     /locus_tag="PLE39_03070"
     CDS             complement(8297..8944)
                     /locus_tag="PLE39_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HNE67332.1"
                     /translation="MHKLEQITDGIRKTFDARTSARDHALAQARQLTRACSLAIRAVH
                     RDEADVMNAQLQEARQLADTLRASLAAYPDLFYAGYTQDALKEFVEANVTCALIRNEP
                     LQTPEELVVEPATFLNGMAEVVGELRRRILDILRHGYSPEAERLLSYMDEIYSVLVTM
                     DYPDAITNGLRRQTDLARSIIEKTRGDITFSLRGEHLTQAISRLSAQLNHDQPSD"
     gene            complement(8947..9285)
                     /locus_tag="PLE39_03075"
     CDS             complement(8947..9285)
                     /locus_tag="PLE39_03075"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNE67333.1"
                     /translation="MTTPPIPSKPTGPILELPADLELVYANLARIAHSPADIVLDFAH
                     LLPGEPRAKIKSRVVMTPLSAKLLVRALTENIARYESAFGEINIPSNNSLADNLFRPF
                     QQPPEPPKEP"
     gene            complement(9348..9779)
                     /gene="ssb"
                     /locus_tag="PLE39_03080"
     CDS             complement(9348..9779)
                     /gene="ssb"
                     /locus_tag="PLE39_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNE67334.1"
                     /translation="MYHTIIIVGNVGKDPEMRYTPSGQAVTSFSVATSRQYTAGNGEQ
                     VKETIWFRVSTWGKTAEVCNQYVKKGSKVLVEGRLTPDKNTGGPRIWSKQDGTAGASF
                     EVTASTVRFLSSRGEAHESGPIAGGGGMEMAELPPEDDIPF"
     gene            9932..10465
                     /locus_tag="PLE39_03085"
     CDS             9932..10465
                     /locus_tag="PLE39_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNE67335.1"
                     /translation="MSETTPKRYHPLHVAIHWLMALLVFLMLGVGKVVMPGISPEDPQ
                     KVMMLQSHTYIGGAIAVLFVIRLILLFTTRRPAPADAGNAFLNFVGKAVHLLLYLFLA
                     GMAVSGAGLFQMAGLPDVFSAAKPYPSNFFDYLPRMGHGLLSWLLLALIALHFGAAMY
                     HQFLKKDNLLARMWFGK"
     gene            10521..10886
                     /locus_tag="PLE39_03090"
     CDS             10521..10886
                     /locus_tag="PLE39_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67336.1"
                     /translation="MNWQITYIADENLIYVKTSGVVTSEAANSMLKEIVEVAVCHQCD
                     RQIIDHSGADFALRLFELYERPSINVRIGLSHKWKIAMIFSELTQNTFFMETVFRNRG
                     YNFRQFDNLEKARTWILSE"
     gene            complement(11125..11679)
                     /locus_tag="PLE39_03095"
     CDS             complement(11125..11679)
                     /locus_tag="PLE39_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE67337.1"
                     /translation="MITSEVLNPLILSAFTKEITWLEAASRFSLDELIETFYYTRERT
                     EENLAPLTDAQVAYTSQAHSLWSLSESITHLIYTQGFYHNKLLEISTSQLPHMVEAAR
                     GFGEGARQNVSANDLRVSLATATERIHATIEQTRHAHDPQKTEISSAFGICNYQTWIL
                     LMLAHEVDHLRQMVAMRRLARTST"
     gene            complement(11710..12897)
                     /locus_tag="PLE39_03100"
     CDS             complement(11710..12897)
                     /locus_tag="PLE39_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNE67338.1"
                     /translation="MNKTFVKPRYDSGGFAGTPNRIIEAFASKKYDAVVLFFIDAFGW
                     RFYERFQDAPFIKRIERTGKIEKLTSQFPSTTAAHVTTMHTGLPVGESGVFEWQYYEP
                     QVDHIITPLLFSFAGTRDRDNLKNKKVNPASIYPKGILYPELAKMGVESFNFGLRDYT
                     PSTYSSVVMEGSEPHAFKTLSEALVNVGLLLEKQQNPTYIHLYFDKIDALAHEYGPNA
                     PQTEAEIQTFLLMMEHYFETIFNGKKRILFLMTADHGMCEIDPKTTIFLNTHPEFDGI
                     QRLIRKNRKDRLIVPAGSARDMFLYIKDGFLDEAQDFLARRLEGRADVVKTEALIADG
                     YFGKEISPRFLERVGNLVILSYRYESVWWYEKDKFEQNFYGHHGGLTPQEMETILYSC
                     ETG"
     gene            complement(12938..13423)
                     /locus_tag="PLE39_03105"
     CDS             complement(12938..13423)
                     /locus_tag="PLE39_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNE67339.1"
                     /translation="MPTISRPKLPKGYVDHPIAEVPWEHVEKRLTESINYWLCTVYPD
                     GRPHVVPRWGAFLDGKLYYDGSPETRHARNLEKNPNTTLHLESGNDVVIMEGTSTPAS
                     KPDLELAQRLSAVYCAKYASDGYAPKPDQWDEGGLYIFTPRQCLAWTKFFENPTKFIF
                     E"
     gene            complement(13426..14334)
                     /locus_tag="PLE39_03110"
     CDS             complement(13426..14334)
                     /locus_tag="PLE39_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE67340.1"
                     /translation="MNRITKIVLGVILGLLVVVAAGLLLKTRSEAYNLLHSPMETRKL
                     PIDTPDDLKLEFESVTVTNPDDMELAGWFIPGENDAVIIMQHGYKSTRSELLNEAEMM
                     HRHGYNVLVTSIRAHDESEGELITLGAYEVQDMEAWYQYLLTRTEIDPEKIGILGNSY
                     GGMLAIQFASQNENIKAVVANSAFSSMQDTVATSVKHFTGLSEFPFVPLIVFWAEQQT
                     GIKIEEINATEWIPLISPRPVFLMQGGRDTVISPVSGERLYNAAGEPKELWFDEKLGH
                     VEFDTRRTFEYENRVAAFFDRYLLGE"
     gene            complement(14368..16701)
                     /locus_tag="PLE39_03115"
     CDS             complement(14368..16701)
                     /locus_tag="PLE39_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNE67341.1"
                     /translation="MTEETPPVEQTPEPQNRGTGALILFLFFALPMPFCLFIYHFVLW
                     STEQSAIISSSAKDFAWAGPLGLLGQAILMSILTALLWYFTKDDRFKPVYAGLLGASL
                     MGFPALALRAIGPNNDQLGFIAQMLLCGIGTGVVILIRKNKLKWDSASLPFGLVVAGI
                     GALPFAIYGSLGLAGDVVLSLFASAAFGLLAAVLIESSTENVFLDGIGIGAVLALLGS
                     SLGYDGAQLLLLAALPSFAFAVAAVMPSRAAAGAAVSMLTFVTLAMFDPTELTFVLGD
                     IGGLAMSAGGWVMVIGWGVSIVALILRFVTATSSRSVTKRAIGWAGAGVAWILVVAVF
                     FIFGNPGSYGDRLFVILKDQADLSDVAKIKDRDERLTEAYTRLTAHANETQSELRAFF
                     DKAGIEYTPYYLENAMEVRGGTLVRLYLMTRPEVDRVIPSPRLRAAPEDAAASEGAMD
                     VESNAQWNIAMIGADQVWNEFGVTGEGIVVGQSDSGVDGNHPAIREQYRGFNSGDDYN
                     WFDPWDGTTSPNDEGGHGTHTTGTILGVNGIGVAPGAQWIGCVNLDRNLANPALYLDC
                     MQFMLAPFPHGGDPLKDGDPTKAAHVLNNSWGCPSIEGCDPNALLYAANNLRDAGIFV
                     VVSTGNDGPNCETVESPLSLYDSVFSVGAIDILGNMADFSSRGPVTADGSGRTKPDIV
                     APGVNILSSIPGGYYAEFSGTSMAGPHIVGVVALIWSAQPELIGDIDATEQLIIDTAK
                     PYTGNISSGCFTGSNPSNAFGYGVVDVYAAVKQALGK"
     gene            complement(16844..17497)
                     /locus_tag="PLE39_03120"
     CDS             complement(16844..17497)
                     /locus_tag="PLE39_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67342.1"
                     /translation="MKNILKPVRWNTFFRDFIRIEIGFMLFGLAIVLMIQGNLGTSAW
                     AVLEVALAHKIGISIGTMTVMMGFIVLSGALIMREKIGWGTLGNILSIGPWEDLWLSI
                     IPSVKDNLPLQGGMLLLAILLMGIASAIYIGVDAGAGPRDSMMLAIKRTTGISIRVAR
                     AIIEVTVVTIGWLLGGPVGIGTLVYALLVGPSVQWGFKIFHVQPHGPEALVPQAPED"
     gene            17545..17988
                     /locus_tag="PLE39_03125"
     CDS             17545..17988
                     /locus_tag="PLE39_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE67343.1"
                     /translation="MTQIIEVQREQFTISTDPARLNLETILDFFTRAYWTQGRPPERT
                     KRAIENSLVFGVYEGTKQIGIARVVSDLSIFAYLCDVFIHEDYRAHGLGKWMLETIMA
                     HPDLQGIRRWTLVTRDAHGLYKQYGFDALTNPDGWMEMIRPFPGE"
     gene            18076..19092
                     /locus_tag="PLE39_03130"
     CDS             18076..19092
                     /locus_tag="PLE39_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNE67344.1"
                     /translation="MKIKNWQFWLGVLISVIFIWLSLRGLKLNEFWGTVQKANYLWLI
                     PGIGVYFVGVWVRAWRWHYLLKPIKEIPTKTMFPVTTIGYMGNNIYPARAGEVLRAVI
                     LKRKEGVPVSASLATIIVERIFDGVVMLAFVFVNLSELAKLTGASGFVGNIQQVAIIG
                     TGVFLGALIVFLLAAMFPHTTAKIGLWLIEHLTPKRLHTKIIGIMNKFLDGLASLRSP
                     LNVLMVFLTSVIIWLLETGKYWFVMHAFSFNVSFFALMLMNGIVNLATTIPSAPGYIG
                     TFDAPGIAVLTAYGVDQATAAGYTLTLHVALWLPITILGAYYLAREGIKWSDTLREET
                     ENTD"
     gene            19097..19684
                     /locus_tag="PLE39_03135"
     CDS             19097..19684
                     /locus_tag="PLE39_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HNE67345.1"
                     /translation="MTTRIYVDWSGDPGFKFRQGSSELLVIAAVMADEEPNIGNLREK
                     LSLPDNYEFHFSKADHRIREHFRNYLNTELEIPGVVVLRADKQMFSLNMRQKRGEQVV
                     ADLIAQCVVNLPAELLHDSTLYYDGEKEQESFKNTLRVTLSQTLHSGVFLRSVKAVPA
                     SRNDGLQVADMLAGFVRNNPSAIKSSIVKLIHYPD"
     gene            19820..21094
                     /locus_tag="PLE39_03140"
     CDS             19820..21094
                     /locus_tag="PLE39_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE67346.1"
                     /translation="MKIAIVGAGYGGMVAAWDLKKAGHDVTIFESAEYAGGLASGFKE
                     PHWDWSVEKFYHHWFQSDGEMLGLIRELGLEDKVRFPRPLTVMYYKGKWYPFDSILNA
                     LLFPGLGWGLNKIRFGFVGLFLRLTNNWRALEKVTADEWMMKYAGRQVYEQMWKPLLV
                     GKFASFYKDVNMAWLWARMHARTTRLGTFEGGFQKFADLFAEKLRAQGVQIKLGVSIK
                     SIKRDQASGSLLVDDQPFDKVLVTSSPSLMAKLCPELPESYLKGLLGLKSMGAVVMTL
                     SLAHPLSKEGYYWFNVPKEEGYPFLALVEHTNFVPKENFGGDHIVYAGDYLELGHEYF
                     SMSDEQLLERFIPAFQKFNPEFSRDWVKKIWVHKTNYAQPVPLVDHSKNIPEIQTPIE
                     GLYFASMSQVYPWDRGTNFAVEIGRKAARMMK"
     gene            21185..21811
                     /locus_tag="PLE39_03145"
     CDS             21185..21811
                     /locus_tag="PLE39_03145"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNE67347.1"
                     /translation="MWFYLLQGIGFGFAAASQPGPFQTYLISQTLTRGWKHTLPAALA
                     PLVSDGPIILLCVLVLSQVPAWMQRGLYIAGGLFIIYLAYGTYRTWENFDTAAQTTAP
                     AGEVQQSVLKAAMMNALNPNPYIFWTLVTGPILLSGWRQSPILGVGFLAAFYLTMIAG
                     LAAVILIFGFAQRFGAKFNRMMLGISAIALFCFGIYQLWLGLFPKEIL"
     gene            21808..22113
                     /locus_tag="PLE39_03150"
     CDS             21808..22113
                     /locus_tag="PLE39_03150"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNE67348.1"
                     /translation="MNIVLVHVHVKPEFVEAFKQASIENASNSIKEEGIARFDVIQQN
                     DDPTRFILVEVYKTAEASAAHKETAHYAKWRDTVAEMMAEPRQGIKYTNIHPEDSGW"
     gene            22117..23274
                     /locus_tag="PLE39_03155"
     CDS             22117..23274
                     /locus_tag="PLE39_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNE67349.1"
                     /translation="MPAFEFATANRVIFGAGKLSELGNQVKGRAKRLLLVRGNSSDAI
                     PRVKEILSGQDILFNEFALHGEPTVATASEGVDLALQHGVDMVVALGGGSVLDAGKAI
                     SALTTNRGDVFDYLEVIGKGQPLVNAPLPFIAIPTTAGTGTEVTRNAVLGSPAHGVKV
                     SLRSPMMLPSLALVDPALTYALPPAITASSGLDALTQLIEPFVSVKANPMTDAICREG
                     IRRAARSLRHAWHNGAEVAAREDMSLASLFGGMALANAALGAVHGFAGPLGGMLNAPH
                     GAICAKLLPLVMEANLKALESRYAGHVAIERYTEIARMVTGNESASAWDGVQWVSDLV
                     RELKIPPLSTHGMNAAHIPEAVQKTLNASSFKGNPIPLNEGELREILEKAL"
     gene            23332..24405
                     /locus_tag="PLE39_03160"
     CDS             23332..24405
                     /locus_tag="PLE39_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67350.1"
                     /translation="MCNGHNHGAGCSCGFGPPYLGPGKPIPKGGRGDHFRGVIRERKR
                     ENWAAVGAINKEKIEQSLGQLRLGARWRKAILERYSKADFPIASSVWEGYSEGRKRGA
                     AQRMLRAVGLRQKIVEELEPIHLEIPLFRLQPPRTENSKVIYQERQIKERGWDISVKV
                     PGFATGADLSMYLEGKGLVETSHKECKQIAFPVTIRRYRVDLFLGNLCIARNKLIAEA
                     GNQKTGQIFSRTIIACKKFIPPSPQSEMIVEYDLSKDECGNSKFFLGWGEKMRRNAEV
                     TIPIPGIPSGIQVSISIQSDIQLEFDLEPKIDYKLYPTPEGIGISWEVSRGKAKRKNI
                     RNDKGISQFKQRPKKLIYVKRHV"
     gene            complement(24688..29964)
                     /locus_tag="PLE39_03165"
     CDS             complement(24688..29964)
                     /locus_tag="PLE39_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE67351.1"
                     /translation="MEITDRKKAEEALRESEAKYRLLAENISDVIWVLDVESSRFRYV
                     SPSVTSLRGYSVEEVMTEDMAAALTPVSLEYLSQVTPERLGKFQQGLSKSYTDEIEQT
                     CKDGSTVWTEVISRYVIDPETNRLEVYGVSRNITERKKAEAAIRLIEKRNTALIEHAP
                     DGIALVDADGMFLFASPSAYRMFGYDAAEIVGTQSREKVHPQDVSLLANLRGKLLSNP
                     NVPQTVEYRFMHKNGEYHWLESTYTNMFDEPSISGIVINFRDVTERKLSEEITRQQSE
                     QIHLLYEASQKLNRTLNLQEIYQTICDFMVTTIPTSTMFISAFDQETQLITCRAYWIE
                     NKWLDVSNFPSIPLEEEGKGTQSLVIRTGKAMLLNDFQSYMKNTSTVYQVDSDTNEIS
                     SEDDETPEEEVTRSALIVPLKSDGIVRGIIQVSSYRQDAYTENQLKLLESLALHIASA
                     EQNAILYNQMLKELNERTQAEDALRASNETSQAILNASTESVFLMDTSGNVIATNETT
                     AARLGKQVSDLAGTNMYDLIPESVGASRKKQVEMVIEKRKPVIFEDERFGVWLENSIY
                     PIIDGDGTIRRVAVYGRDITDRKKAEHALRESEGRAQAMLQAIPDLMFRLNREGVFLD
                     YKADAKDLHEQTVSLIGTRNRDIAPVEFADLIDHYIAKTLEANQLQTFEYQLPVPGKG
                     LRDYEARMTPSGTDEVLAIVRDITERKQVEQNLRETQSRVELALKGANAGMWDWNVQT
                     GETIFNERWAEIIGYALEEIEPVSIQTWADFCHPDDLEISDGLLKKHFAGETQFYECE
                     VRMKHKNGSWVWVFDRGKVMEWDSEQRPLRMIGTHLDITERKQTEEALRAREELYRMI
                     STINADYVFSEDVDMNGKTSTKWVGGGFEAITGYTFEEYSKQGGWLSILHPEDREKDA
                     ADFAALKKNQKIVSVLRMIAKNGDIRWVQVHANPLWDSELQCLTGVYGAVQDITERKK
                     AEDALHASEEKYRVLIESLDNMIASVDNDGRFLYVNDMAAAQMHLTPNELIGKTMYEL
                     FPPEVTARQMSDIQRVIHGDQGITFEAPSFSSTAPRWYRTSILPIHDEDGLVTQVLIN
                     STDIHNLKTAQQELQDLNRTLEERVKQRTAEVQDLYDNSPIGYHSLDADGTLLFINQT
                     HLTWLGYTREEMIGHSITEFLTTRGLEGFKEIYPSFLKNGMVRDVESEFRRKDGSIVP
                     VLISATAVYNEAGEFVMSRTSVFDNTGQKAAEQALRNANLELARAMRMKDEFLASMSH
                     ELRTPLTGILGLSEALQINVYGPINEKQQNTLANIEASGRHLLDLINDILDVSKIEAG
                     KLELQMEACSLSEICQSSIQLTKGMAGKKRQTVNFTMNPANISVRGDARRLKQILVNL
                     LSNSVKFTPEEGMLGLDVTANDSEGLVYISIWDKGIGITPEDLKKLFQPFVQLDSSLS
                     RQQSGTGLGLALVQRLVELHGGSIDVQSVPGEGSRFTVTLPCQPADKASQDVENPSQL
                     KFHQALIIEDTPADAEHLARYVKSLGITAIMHTSGKDAVERVIETQPDVVFLDLNLPE
                     VSGWDVLTQIKNSRNTKHIPVVITSVDDDKSKASKLDADGYLVKFFTLSDLRTALSHI
                     QKPSLASSEGHINPAAATVATIMIVDDNEVNINTLADFLAMQNFNIISVKSGVDFLAQ
                     APHIHPDIVLMDIQMPGMDGLETTRRLRRLSDRRLASVPVVAITALAMPGDRERCIEA
                     GADEYITKPFRLTEIHDLILKMVEERKSLTGG"
     assembly_gap    30010..30109
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<30110..32643)
                     /locus_tag="PLE39_03170"
     CDS             complement(<30110..32643)
                     /locus_tag="PLE39_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE67352.1"
                     /translation="MSKQKTKLQLQQELELAQKRIEELERKLPAPDENEERFSRFFQA
                     SPAQMAITDINTGKYLDINKTFLETLEFTREEVLGKTALELNLFFDPDQRGALLGKMK
                     EQGFLRDEDVLVRTKSGGLRHGIFSAEYIQVNSQSFLLTVMNDITERKLAEERLSESE
                     ARLKGFLDSAPDAMIIVNAQGKIILANLQTEKLFGYKQSELLEMKIEQLIPERLRDAH
                     PQKRDYYLQNPQHKTTGIQQGIYALRKDGSEFPVEISLSHHKMKNEVVILSAIRDITE
                     KKQAEVSLLKSTQILHESQLMARLGSWTADLKTGIFESSSEDAQLIGWAPGGHSLNEL
                     MNIIHPDDREFVQVSWSAALQGGPFDIEYRIIAGDDIRWLRVKASIKFDNHGVPASAI
                     GVTQDITERKQSAILQETVYHIANAAQTTESLQDFFKGIHHHISNVMAARNFYIALHD
                     EEENLLHFVYSMDEKDPIRESMPPDKGLTSHVLRTGESLLYVYNQTGQRIEVVGTQPK
                     VWLGVPLIARNKIIGVMAVQNYSDPHAYGIREQRILEFVSSQVATAIHLKKATDLLQK
                     SQTSLEMALATANLGSWELDIKRGKGMVWSKEMFHLFRRDPARGIPELAEFMEMVHPD
                     DRQRLLNAQEEAFQSGQVATLEYRATPIPGETRHYKVTIQGILDPEGRTIHASGTVLD
                     ITEIRQAFEKIQESEEKYRLLFETMVQGVVFQDENGGIIHANPAAERILGLTLDQMQG
                     RTSIDPRWKSIHEDGSDFPGDTHPSMVALKTGKPIHNVTMGVYNPAFDSYNWININAT
                     PRFNGNSEKPHQVYATFEDITERKLAENSLLASEKKLKSLIASQTH"
     gene            complement(32861..35566)
                     /locus_tag="PLE39_03175"
     CDS             complement(32861..35566)
                     /locus_tag="PLE39_03175"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNE67353.1"
                     /translation="MNWHLLGIQESLSNLNSDQQNGLTESESSARRKQYGPNELIERG
                     GRTPWQILWAQLTATMVLILIAAAVLAGILGDYKNTISILAIVALYAILGFVQEYRAE
                     QAIAALKKMSVPNVRVLRDGSLKEVAANQLVPGDILQLETGNVVPADVRLLEAVNLRI
                     QEAALTGESEAIEKHTSALSNDPNLALPLGDRRNMGYMGTIVTQGRGLALVIATGMQT
                     ELGKIADLIQKVEQENTPLQRRLDALGKNLALIGTGIAILILILGLLRGDELRHMLLT
                     AVSVAVAIVPEGLPAVVTITLALGAQRMLQRNALIRKLPAVETLGSVTVICSDKTGTL
                     TENRMTVVVLDVADHALDLTEEIERDGTLRATRGLGAPAQSSLSLAVIGGALCNDSKL
                     IDTGDDRFHTLGDPTEGALVVAAAKMGYWKDSLDPSFPRAAELPFDSERKRMTTVHHL
                     GQYDPTVLAGLEIGDHRYIAFTKGGVDGLLDITSHIWSEGKSQILDSGWRSKIEAANE
                     RLAKKGMRVLGIAFRLMNSIPEVIQTDLEQNLTFVGLFGMIDPPRGEVQEAVATCRAA
                     GIRPVMITGDHPLTAIEIARQLGITENGRALTGVEIENLSSTELRSVVDEVNVFARVA
                     PEHKLRIVQALQENGHIVSMTGDGVNDAPALRKADIGVAMGITGTDVSKEASDLVLLD
                     DNFATIVASVKEGRTIYDNIRKFVRFSVAGNIGKVLVMLIAPFLGKPLPLLPLQLLWL
                     NLLTDGLLGLGMGVENPEPDTMKRKPYSPTEGVFSRGAGTQTIWVGAMIGALALGLGS
                     WYFFTGRPEWQTMIFTSLAFMQVFQAFASRSSKESVFKMGLLSNSLLAGMGLLVVVLQ
                     LMVMYIPVLAHFFEVMPLSGCDLAIAASTGVIVLTVMELEKKLKK"
     gene            complement(35563..36234)
                     /locus_tag="PLE39_03180"
     CDS             complement(35563..36234)
                     /locus_tag="PLE39_03180"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNE67354.1"
                     /translation="MFDQIETTILSALQTIFDQFGWAGVFGLMVFENATGITPSEIIL
                     AFAGWMLIERHGIAPTFVLIAGVYAGLGSTLGASITYWIARLGGRPVIDRFARFFRID
                     PALINKAEEQMQKWGSGLVLIGRIIPGIRTLISIPAGLAKIPFLKFFAATFIGAYIWC
                     TLLIGAGYVLGHEWMLVSEAIKQHLPLVLTLGLLAMAAVVVYQYRARIPALAWMNTKR
                     MDNES"
     gene            complement(36463..37560)
                     /locus_tag="PLE39_03185"
     CDS             complement(36463..37560)
                     /locus_tag="PLE39_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNE67355.1"
                     /translation="MKWQWKLGKFAGIDVYVHATFLLLIGWVGYSHWLEHRNWGEVLN
                     GILFILALFVCVILHEYGHALTARKYGIKTRDITLYPIGGVARLERMPEKPIEELWVA
                     LMGPAVNVVIAAGLFAYLYLTSSLVPLNELTIASGNFLERLMTVNLSLVLFNLIPAFP
                     MDGGRVLRALLAMRMDYVRATQIAATIGQGMALLFGLVGLFGNATLLFIAFFIWIGAS
                     QESSATQMKNAISGIPVGRAMLTEYKSLSPRDTLARMSQLLLAGSQHDFPVIDGERIV
                     GVVTRDDFLMALTQHGEGIAISAVMKRDLPEVDSYEMVENALLRIQESGVPALPVTHG
                     GQLVGIVTAENIAEYLMIRSALRASRMAVPL"
     gene            complement(37799..39010)
                     /locus_tag="PLE39_03190"
     CDS             complement(37799..39010)
                     /locus_tag="PLE39_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE67356.1"
                     /translation="MDFNNTNDAVTRRIKAMTDRLDYLKSNDLYFYNQPVEEILPGNK
                     VRVRGREMGMYASYSYLGLVGHPRINEAAKKAVEKFGTGTNGVRTLAGTLTIHSELEE
                     TISNFKHTEAAITYTSGYATNLTVISTLMGRGDYVFSDKINHASIVDGCLMSGAEFRR
                     FRHNDMEHLEGLLKNAPADVAKLVIADSVFSMDGDIIDLPTMVTLCKKYNAWLMIDEA
                     HSVGVLGEKGTGIEEYFNMYGEIDIKMGTLSKTIPSVGGYVAARKEIIQYLRHASRAY
                     IFSAALPPAQAAAANEAFKVILDEPWRIERLNQNTKQFIGGLKSMGFDTLLTETAIVP
                     VLCGEDDMAFAMTRECQHNDVFVLPVVSPAVQEGLARLRATVTAAHEPSEIERAMDVI
                     GEAGKKLGLLK"
     gene            complement(39151..41196)
                     /gene="ligA"
                     /locus_tag="PLE39_03195"
     CDS             complement(39151..41196)
                     /gene="ligA"
                     /locus_tag="PLE39_03195"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNE67357.1"
                     /translation="MATKSSSRYEELKEQVNFHNYRYHVLDAPVISDLEYDRLLNELK
                     QLEADHPEWITPDSPTQRAGARPADRFEKIRHPAPILSLANAFGADDARAWFERIKKL
                     DDRVEKAKFVVEPKIDGLSVVLHYRDGMFMQGATRGDGEVGEDITSNLRTVRAIPLKI
                     PVGSEQGRQSSLHPFQVPKHLVVRGEAFIPNKDFEALNKKLEEAGEKTYLNPRNTAAG
                     SLRQLDPQLTASRPITLLVYQIVHSEGGKVPTSQWEILEYLKALGFPVTDVARRFNDL
                     EAAIAYTETFSTGRDALPYEADGMVIKIDDLTLAADLGFVGKDPRGAVAFKFPAREVT
                     TTLNDIGVAVGRTGVLTPYAMLEPVEIGGVVVERATLHNFDYIAEKDIRAGDRVLVKR
                     AGEVIPYVIGPIVDARSGKEKKYKPPSKCPACGQEVEHLEGEVAWYCVNAACPAQLVR
                     NVEHFVSRGAMDIVGLGIKIVEQLIEAGLVKDVADLFSIKKEQLLELEGFAEKKAENL
                     LGAIEQAKGQSLNRLIVALGIHGVGEVMAGDLARTFGNLSTLSKASAEELQQMDGLGP
                     NIAESIVDWFAQNANQKLLKKLKTAGVWPEMKKDEKKKEGAFTGQTFVVTGTLPTLSR
                     DGVKEFIESNGGKVTDSVSKKTSYLVLGEAPGSKLDKAKSLGVKVIDEAELRKLAEG"
     gene            complement(41251..41583)
                     /locus_tag="PLE39_03200"
     CDS             complement(41251..41583)
                     /locus_tag="PLE39_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67358.1"
                     /translation="MNETPFELAVKELEAGTEDIRPSDIVSYSEPLRSALNFVIRLGR
                     FSMTEFQEKLEFTREETKKISDILVARNLFAISRFTTAEETYFESRLTAMTRPLTRPP
                     SDIWKKIE"
     gene            complement(41604..42359)
                     /locus_tag="PLE39_03205"
     CDS             complement(41604..42359)
                     /locus_tag="PLE39_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNE67359.1"
                     /translation="MSTIISVHSFRGGTGKSNTTANISALLAVDGARVGVVDTDIQSP
                     GIHVLFNLDESEMGHSLNDYLWGKCSIEETAHDVTSHLGAEVKGQVFLIPSSIKPGEI
                     ARILREGYDVGLLNDGFRDVVEKLKLDYLLIDTHPGLNEETLLSIAISNALVIILRPD
                     SQDYQGTAVTVDVARKLDVPKMLMLVNKVPPQFDLEDVRTRVEQTYDATVAAVLPHSD
                     EMMTLASSGIFAIRFPDHPMTKGLRKLVDQLKA"
     gene            complement(42464..44029)
                     /locus_tag="PLE39_03210"
     CDS             complement(42464..44029)
                     /locus_tag="PLE39_03210"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE67360.1"
                     /translation="MLPESPLAAIESRLRYLLPAEMYASMWTNPSVDMMIDVHKHLRT
                     LQRILHDYSSRQISSQTMKPGNIKTEWQYGTMMFTDLAGFTKLMEASSNKGREGAAEL
                     LKHLTKYFSTMIAIISKSGGDLVEFTGDALLIMFPKRDRNDDTLHAVRAGLRMQRAMK
                     EFAHIETSAGIVTLKMRIGIHSGRFLTSDIGTPRRMEHVLLGRDVQRAKLAESKGRNE
                     RVNLSPSAYERVLEHFRFEQGEKIENEKDQYYMLVEDDLADNELGEYEITPVSRRMAS
                     STLIDRDKQSVLKEITDLVNSIEPMASFLPFPVLSLLVESAAQRKIDPDFPKPTIMFI
                     NFVGLPEAADRALPGEELKLSTSFSKAFSLINAVVESRGGMLKKVTYHLSGSDIVIYF
                     GVPTAHTNDEMRAASAAVAIRDIVMNIKPPTIGNFQPEIYCMIGIHTGPAFVGEIGDP
                     RGRREYNVLGDTVNTTARLMNRAGRNQIIISESVQQAIETKYECQPLGEASLKGKSTP
                     MALYELVQKTPKP"
     gene            complement(44125..45015)
                     /locus_tag="PLE39_03215"
     CDS             complement(44125..45015)
                     /locus_tag="PLE39_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67361.1"
                     /translation="MSNPTLISPSSPLKRIINAVHAVEAGEYVPGLLGDLINESGELG
                     QLARMLDKMAHGIAVRDHQLRLLRKVIPIGVALSAEKDYNRLLETLVVEAQFFTNADA
                     GTLYILEDNALKFVILRNTSLGMAMGGTSGNEIPFYPVKLYKADGSENRANVVSHAAL
                     THNRINVADAYEADGFDFSGTKAFDTRTKYRSKSFLAIPLTNSEDKVIGVLQLINAKD
                     PATGEIISFKDDDILEALILLATAALDGYIREASLRQEIAKLKIEINESRRSREVAEI
                     TETRYFQELKDKATQLRSRK"
     gene            complement(45114..45395)
                     /locus_tag="PLE39_03220"
     CDS             complement(45114..45395)
                     /locus_tag="PLE39_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67362.1"
                     /translation="MSTKNKWILGIGITLGLIVLFFLPSLWQVFFPSAGFGMMSHGNM
                     GMMNQIGSFPIRGGMGIGMSFLWFFPLVLLALIGLLMAALIKYLRSPSV"
     gene            45493..46101
                     /gene="nadD"
                     /locus_tag="PLE39_03225"
     CDS             45493..46101
                     /gene="nadD"
                     /locus_tag="PLE39_03225"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNE67363.1"
                     /translation="MPPTNIGIFGGTFDPPHIGHLVLASEAVQQLNLSRLLWVLAPEP
                     PHKLDQAITPLSHRLEMLKRTIADNPSFEISRVEIDRPGPHYSVDTVKLFAELYPDAE
                     LTLLLGGDSLRDLPTWRRYTELVSAVSKIGVMRRPGVSFDMPALETQIPGITQKVWFI
                     DALLQNVSSRELRRRVASGETYRYYVLPSVYDYIEANHLYGR"
BASE COUNT        10623 a        11485 c        12546 g        11752 t
ORIGIN      
        1 cgtttgaata accctgcgac aaatgagatt ttccccgact accaaccgaa gatgccgaat
       61 gtgatgaaat aaaaagtagg gacacagcac cgctgtgtcc ctaccgtgtc cctacgacat
      121 tttatccacc aaaatccaca gtgatgtaat ctccgtaagc gccgtttgaa aaataagcgt
      181 agccaacacc catctcggtg gtgttggggt tgaggatcgc atcgcgatgg atcttgtcac
      241 tcatccacca actcatcgcg gcttcgggtc cgccgccgcc gtagatgatc tcttcataga
      301 attggcctga ataccccgaa gccagaaccc gcccgtatgc cgatgagccg tctgagcctg
      361 tgtggctgat gctgctgttg caggccatgt cggcggcgtg tccctgcgcg gcggaagata
      421 gctcggcatt gatcgtcaat tgcggcaggc ccgcattggc ccgctccgcg ttgatgagcg
      481 aggcgatctg attcacgtag cccgcgttct gactgaaact gcaattcgcg ccgccgccgt
      541 tgcttacggg tgtgattcca accgcctggg ttgggttgcc tcccgatgtg gttgggacaa
      601 tggccccgct ccccacgatg atcttcaccc aaaatgtttt ttcggtgccg atgggcaggg
      661 catttccgtt cgcgtcgttc agggtgaaat accctgtgaa ggtgccgttg gaggctgggg
      721 cggtcaactc caccgaggct tcgactcttt ccttcgcaag cgtatcagga atcggcgcgg
      781 agagaggcgc gttcattttg tcgcccgatg aaaacttgag cgtgtatccg atccacgggc
      841 aggttccgtt gttgatgaat tcccaggtct tggtaaattt ctcgcctgct ttcacctgcg
      901 tgttatcagg gatggttaca tcgcgcacga gcacagcgct gtcggtgcaa ttggcaggcg
      961 cggtgacggg aatgcgtgtg ggtgtgatgg tggcttcggg cacaggtgtg agtgtgggcg
     1021 gcacgtaccc tgcctgggtg ggcatcaatg ttgaggttac gaaatcaggc tgtgcggtgg
     1081 gttgtgcgtt cgaatccacg gagatggaaa cgcaggaagc caaaatcaac acactgcaaa
     1141 gaaaaatttt cttccacata aaaactccac ttgcttcaaa acatcatcat ctgtcccgca
     1201 gatttttcca cagggtcact ggcgatctgt tcttcaaaag agtcacgcgt cagaatcggg
     1261 acctcaagaa tggcgcgcaa caaacgatat ttcaccacgc ggcgggtttt caattgcgcg
     1321 gcgagggcgg ggtcgcgttg aagtttgtag gcggcgagtg cggcgcgccc accggggatg
     1381 acgagaatgg tatctggtgc ggtcgtttca agcgcacgcc cgaacagcgc ggaggccatc
     1441 acataaaacg agcggatggg ttttccattt tcaagccagg tcagggtttt gtctgtgcgg
     1501 ttggttttgt attccaagcg cctgccgatg gcttcgatca gggcaaagat gtcgttcaat
     1561 tcactgcgcc gtgccgatgc aaggtcttcc gcgcgcagtt tccatccgcc tgattctttt
     1621 tctgcatatg aattcagcac agcgtagatc aaccctttgg agggcgtgag caatcccgta
     1681 aaacgcggat acagttcatc ttccacttca aggtagatgg catgttgatt cttttgcagg
     1741 aaggtgacga tggccacctc cacctggtct gaaagcgatt cgctttttgc ctgtgccccg
     1801 ccgccgaaat gaaggctcca cgttccagtt tccacatttt cgcctgtcga ataatggacg
     1861 aagcgttcat cggttttcaa tgctgattca aaaagcgcat tcgtttttcg cagggcttca
     1921 tcgaattcat gctcgggttg tttgagcgcg ttcgcttcgg ccagcgcgat taaccccgcc
     1981 gtgtggacgt gtaaataact ggcaggctct ccacgtgatg cgagatattc ctgaatcgaa
     2041 gtttgcaaaa gagagtcatc agccgtttgc ggcttttctt ttttgccgct tttccagatc
     2101 acctgcacgg ggtcatgctc ggtgcgcaaa gccacattca ccagcgaaaa ccccgaagca
     2161 cttgccgcgg taaatgcaga agtgaggaac gatggttcgg gctcaggcag aaaagcaaaa
     2221 aatggaacgc catcgggcag caggtcattc aaatggttga aggctgaatg cagcgcagtt
     2281 gcattccacg cccagtcata gcggcggcgg cgcaaggcca ccctgtaagg ttccacggct
     2341 tccttgcccc acagccagcc cgcccacaag gcagagagag tccaaaatgc ctgattgggg
     2401 cgcggaaccg aaccgatgac cgcagaaatt gggatctctt ttttgacgag ttgtgcgagg
     2461 tctttgaggc ggccttcgta gatgcagatg ccgccgcttt ctggaatttt cttcggccac
     2521 gcttcacacg ggaccgttga acctgtttct gaccaaagag tgagtccacg ctcaagcacc
     2581 atccacacat tgtgctcgcg gaattgactc ggcgtgctca actgcttcgg ccgtggacgt
     2641 tccgcagggt gtgcccagat ggtattgcct gcatcacacg caatgaggac gagagcagtc
     2701 aacgcccgct ttcgctctgc ggatagattt aaactatcca ggcgattgac gatggtggtg
     2761 atcacataca gcggacgggg taaataatgc tgaatggcct cttctgcgta gaaacggtct
     2821 tcatcgttta aggccacaac tttttcgaag gcgcgtgtgc ggtgcaaggt gtcggtttcg
     2881 gcgatctttt ttgcgcgttc gatatcttct tcggtggcgg ggtgctcacc cgagtcgccg
     2941 cagtgtttgc actcgtatat gcgggcgtag ggggcatctt cacctttgcg ccagagaaaa
     3001 gcattggcat ggatctgttg attgcatttt tcgcatttgg tgagatacag agattgcaaa
     3061 tggcccgcga gtctttcgtc cccttttttg acggcgccga ggtctgccag tgcggcgatg
     3121 aattctgatt ctggcggcgg gttggcgaac atttccagca gaaaacgcgt gatcgggttg
     3181 tttgctgtca ccagcacacg ggttccgctg cgggcagctt caaggacgag tcgcggggag
     3241 aatccgaatg ggtcgagcag ccaggtaccg ggtccgcttt gagccgcacc gaagggctga
     3301 gtcgagagcc agccggagat gactccctct tcgagcgggg gcagaaagcg cgagagcggt
     3361 cccgggtcgg cgggtttcat ccctggaatg taaggttgaa aatccatctt gggttaagta
     3421 tacaacctat aaaggatcgc aaattggctg gctgggtgtg gacttcatgt caatttcgat
     3481 ggcagagagc atgatctgaa tgccgaggat gacgcataaa gtggggagca tcacagtgcc
     3541 tgtgggggcg gggatgttgc ggctggcgta gtcgatccat ttgatggcgc cgaagatgaa
     3601 accaaacagc agcagcggaa tgctggtgac gatgtacagg gaaatgatgg agaaatcgta
     3661 gatgtagtac tttaacagaa tgcgccgaat cagcgtggaa aaaagtttaa gcggaaactc
     3721 caaaaggctg cggcggatgg aaaggtgtga ggtttcgttc ccgtatcggg cgggcatggg
     3781 cacatccatc acaaatgcgt tgacgaggta cagattcgcc agcagtgagg tttcgaagaa
     3841 ataacgttta tcaatggctt cgaggggtag ttgtttcagc acttcggtgc ggatggcgaa
     3901 aaagccgttg gtggggtcaa agatattcca gtaccccgtg gcggctttgg tgagaaaact
     3961 cagccccagg ttgcccacac gccgtaccat gggcatattg cgtagtgaaa cgaaatcacg
     4021 aaaacggttg cctttggtga aatcggcttt gccgaggatc aacggggtaa cgagggcggg
     4081 gatgtgagcg gaatccattt gcccatcgcc atcgagtttg acgacgatct ttgcgccgag
     4141 ttccagcgct ttgtgaaagc ccgagatcat ggctccgccc acgccctggt ttctctcatg
     4201 gcgtatcagg ataatgcggg ggtcttgctg ggcggcggca gtcaccaact ttacacttga
     4261 gtcaggtgat gcatcgtcca caacgatgat gtgttttatg taggcaggca ggtctttcag
     4321 cacggttgtg atggtgtcct gcacgcgata ggcagggatg acagttacaa taccgttggt
     4381 ggaaaaatcc ataaataaga ttgtacttga aaaagtgata gaatcagtca aaggaaaaat
     4441 catggccacc ggggaacgaa ttcttcttgt tgaaagtgat ccagatatcg ttgatctgat
     4501 cgtcaggcag gcattacaac cgctgggata cagggtgggt acagcgggcg atgccgcctc
     4561 tgcgattaaa ctctcgatgc agactccccc ggacctgatc ctcgccaatt tgaatttacc
     4621 aggcctgagc ggaaaagaca tgcttgccgc gctcagtgcg cagggcgtga aatcccccgt
     4681 cattgtcatt gcagaaaaag gacaggaaac cgacgcaatt caggcgtttc gccttggcgc
     4741 tgcggatgtc atcttttggc ctgtgcgcga tgcggaggtc gtttccatcg ttgaacgcgc
     4801 tctgcgtcag acccagcaat cccgcgaacg tcaaaaatta gatcgtcaac tctcggccat
     4861 gaacaccgag ttgcagcgcc gcctgcgcga cctgaccacc ctgctatcca ccgccaaggc
     4921 ggtcatctcc attaccgatc aacgcctgct gtttgaccgc attctcgaaa gcgccatgca
     4981 agtggccgaa gccgattatt gctggctgac cctgcgcgaa gagaaaggca atgcctattt
     5041 actgcgcgcg catcgcaacc tgcccgaggc ctgggctaaa aagatgaacc agcctttgga
     5101 tgacggcatc agttctctgg tcgggttttc gggcgagagt ttggtgatga acggctcgcc
     5161 tttgcagaaa ttcaaaattg cggcgttggg taaatcggtg ggtgtcattc cgatcaaaat
     5221 ccagggtgag gtcatcggct tgctgatggt ggctcgcaaa atagaccgtg agatcgcccg
     5281 cgattcacag accatgcttg aagccatggc cgattttgca tccatttcac tggtgaatgc
     5341 ccgtttgttc cgagctcttg agcagagcgc cgaaaccgcc cgtatgaacg aaaaacagcg
     5401 ccatgccgcc cttgacctgg tgcgcaccaa tatccgtaat gaaattcaat ctgcagggta
     5461 tccgctcgac cttgtgttga ccgagacccc gggtccgctc acccctgaac aaagaaaggc
     5521 acttgaacag gttcaagctg ccttgcaacg ccttgccagc tcctcagaga aaacgaccat
     5581 cccaaataaa tagcaggcat ctcccatgtc aaaacaggaa ctgatcttat tagcaatgga
     5641 cgcatccccc gttttggggt tgatggagcg tactttgcgt tcggctggtt ttgacgtggc
     5701 gattgcccac gacttggtag ggttgaataa atgtctgcat gaggccattc catctttgat
     5761 gatcgtcggc gagaaatttg tcgatcaagg cggttttgcc attgcaaaag aaattctcga
     5821 acgttttccc accctgccga tcatttttta tgccgagcag gataccaccg gttatgcaaa
     5881 gaccgcactg aagatgggtt taagcggtta cctctacccg ccgctcaagg tgtcggatat
     5941 taccgatgaa gttcatcgca gccttgaacg ctccagcaaa ctgggcgatt ggctgcgccg
     6001 tgaagtccgc cgcacaacct cgtcgctgac ggaacgggcc aaactttcgg aaagtgaacg
     6061 ctccaagctg gatgcgatca ttgcgaatat tcaggatggt gtcatcgttt tggatgacag
     6121 gaagttcatc ctgcttgcca accacgccat ccacgatatt tttaatttcc acgatcggga
     6181 aatcgtgggc aggccgcttg cagacgccat aaaaaacgcg gacctgaccg cccttctcgc
     6241 ccgggcaggg gacagcccgc tgaaatatca tgagatcaat tttgacgatg gccgcgtgtt
     6301 caacgcccaa tacacgccca tcccaaagat cggcggggcg atcaccatgc aggatatttc
     6361 ctacctcaag gaattggacc gcctgaaaag cgatttcatt cacactgtgt cacatgacct
     6421 gcgttctcca ttgacctcgg tcttgggata caccgaactg atcgaacgca cgggtccact
     6481 gaacgccaat cagttggagt ttcttcaccg tttgcagggc agcattcaac acatcacgac
     6541 tctgatcaac gacttgttgg atttgggccg cctcgaagca gggtttgata cgcgccgcga
     6601 ggttgttcag ttaagcgggg tcttgcacta ttcgctggat atgttcgacg gccagttgaa
     6661 gaaaaagaat atcacccttg tcaccgatgt ggccccggat ctgcacccac tacgcgcgaa
     6721 tccgattcgc atccgccaga tgctcgataa cctgatcggc aatgccatca agtacacacc
     6781 ggatggcggg cgggtgacca tcaatatgtt catgcaagac cagcaagttg tcctcaaagt
     6841 ggaagacaca ggctatggga tcccacctga agaacaaagc cgtgtgttcg aaaagttcta
     6901 ccgtgccacc aatgccatag aagacattga gggttccgga ttggggctgg cgattgtcaa
     6961 atccattgta gatagccacc aggggcgtgt gtgggtggaa tcagttgtgg ggaaggggtc
     7021 agccttcatc gtacttctcc ctgcacacga ataataaaac gatattctgc ttaaacatgc
     7081 tgccgccggt gatttcaccg gcggcagctt tcgccaaagg aggagacaag aaaggtattt
     7141 tggaggtgtc tatgttccgt ctccgtcctt ataatagtcc acttcttttt tttcagccat
     7201 tcacgaatgt cacattatgg ttgtgacaaa cttcacaaat atttcacagg ctgtcaagaa
     7261 aaaaacgcgc ctgcgagatc gcttcttcca cagttccata ggcttgcgtg gtgatcttgg
     7321 ggtctgggtt gccgctgagc attttcgata cccttctatc tttctggtaa atgcacaaga
     7381 caggtttgcc aagacccagc gcaaaggcga tctcatagcc caccccatgt gaaggtgtgc
     7441 tgacttcggc gatcagggtg tcgcaattcc tgatccagtt cacatcgcgt tcatacacat
     7501 cctgcggcgt gagttggcgt tctttctccg cgatttctga ttgaaccagg tgtgaagttg
     7561 ggatctcatg accgtctttt tcaagggcgg cgacgatctc ctgataggcg gtttcaaatt
     7621 cccgcccgcc agttatggaa catgcgaaat aaatattcat ttgtctttgt tggggaggcg
     7681 agaaagaatc gaagcgttcc gtttttgctg ggccgagatc ggccctgtgc cggtgcgcag
     7741 gctgtccatt tcagagctga atttcatcca gtcatccaca agaatgacaa tgtgaggcga
     7801 tttatctttg aaaaagagcg gcgaaaggaa cagtcgcaat tgcgattgcg gcatggggtg
     7861 gctgttcatt tcgatgacga tcgcggtcat tttggcaaga ggttgaatga tttcagcgcg
     7921 ccagcttgaa accgagttgg gaggaaataa agtgcccatg ttggctgagg tcgcacgccg
     7981 caccctgcgg taagaaatgt tcagccgtag aaaaggcgtg accaatttca aatactccct
     8041 gaacggctgc acataggcgc ttttgcgcat gagccacatc ccaagcccaa tgaacacatg
     8101 cagcccgcca atgctggcga gcgcaagcca gcgccattga tccatgcccc agctatagac
     8161 cgcccaggcc aacccgagca gggcaagacc caaagtgaag acggcggtcc accaacgttt
     8221 gatcatgtgg gtgtagatga caagcggata acggcgtccg cctttttctt ttgccatggg
     8281 aaatttcgct tccttcttaa tctgacggtt ggtcgtgatt caattgagca ctcagcctgc
     8341 tgatggcttg tgtgagatgt tcaccgcgca ggctgaaggt gatgtcgccg cgtgtttttt
     8401 cgatgatgct gcgtgcaaga tcggtctggc ggcgcaggcc gttggtgatg gcatcgggat
     8461 aatccatggt gaccagcact gagtagattt cgtccatgta gctgagcagg cgttccgctt
     8521 caggtgaata gccatggcgg aggatatcca gaatgcggcg gcgcaattcc cctacgacct
     8581 ctgccatccc gttgagaaat gttgcaggtt caacgaccag ttcttcggga gtctgaagcg
     8641 gttcattcct gatcaaggcg caggtgacat tggcttcaac aaattccttg agggcatcct
     8701 gagtgtatcc cgcataaaaa agatcggggt aggccgcgag ggatgcgcgc aatgtgtcgg
     8761 cgagttgacg tgcttcctgc aattgcgcgt tcatcacgtc cgcttcgtca cggtgtacgg
     8821 cgcggatggc aagcgagcag gcgcgggtaa gttgacgggc ctgtgcaagg gcatggtcgc
     8881 gtgcggaggt gcgggcgtca aaggtctttc gaattccgtc ggtgatctgt tcaagcttgt
     8941 gcatgattaa ggttctttcg gcggttcggg tggttgttga aaggggcgga agagattatc
     9001 tgccagtgag ttgttggatg ggatgttgat ttcaccaaat gcagattcat agcgggcgat
     9061 gttttcagtg agtgcgcgga caagcagttt ggcgctaagc ggggtcatca caacccgtga
     9121 tttgatcttg gcgcgcggtt cgccgggcaa cagatgggca aaatcaagga cgatatctgc
     9181 aggcgagtgc gcaatgcggg caaggttggc atataccaac tcaagatcgg cggggagttc
     9241 gaggatgggg cctgtaggtt tggatgggat tgggggtgtg gtcatgggaa gttccataat
     9301 atgagaggca ggtcatgcga gcaagacctg cctcctgtgt gggttggtta gaaggggata
     9361 tcgtcttccg ggggaagttc ggccatttcc atgccgccac cgccagcaat gggtccgctt
     9421 tcgtgggctt cgcctcgtga agagaggaaa cgaactgtgg aggctgtcac ttcaaatgaa
     9481 gctcctgcag tgccatcctg tttggaccag atgcgcgggc cgccggtgtt tttatcgggg
     9541 gtgaggcgtc cttcaacaag tactttggaa ccttttttca catactggtt gcagacctcg
     9601 gctgtttttc cccatgttga aacacgaaac cagatggttt cctttacttg ttcgccgttc
     9661 cctgcagtgt attgacggct ggttgctact gaaaaagaag tgacagcctg acccgatggt
     9721 gtgtaacgca tttcagggtc tttgccgacg ttgcctacga taatgatggt gtgatacatg
     9781 gggggaaatc tccttgtggt tgaggtaccc gccgaaattc ggcggggaaa atacgatagc
     9841 tcgtatttta caccactacc ctatggattg taagaaagca ggcaaaggaa ttgtcttaag
     9901 cgcatcaaaa ttaccacctg agaggaatgc catgtctgaa acaactccga aacgatacca
     9961 cccattgcat gttgcgatcc attggttgat ggccttgctg gttttcctga tgcttggtgt
    10021 gggcaaggtt gtcatgcccg ggatttcccc cgaagaccct caaaaagtga tgatgctgca
    10081 gagccatacc tatattggcg gtgcaatcgc agttttgttt gtgatacggc tgatcctgtt
    10141 atttaccaca cgccgtcctg cacctgcgga tgcagggaat gcctttttga atttcgttgg
    10201 aaaggcggtt cacttgcttt tgtatttgtt cctggcgggg atggctgtca gcggggcagg
    10261 gctcttccag atggcaggtt tgccagacgt attcagcgca gccaagcctt atccatcaaa
    10321 cttctttgat tatctgcccc gcatgggaca tgggttgttg tcatggctgt tgctggcttt
    10381 aattgcgctt cattttggcg cggcgatgta tcaccagttt ttgaagaagg ataatctcct
    10441 ggctcgcatg tggtttggaa aataagcgcg tggtgaagca tggtaaaaaa agaccatgct
    10501 ctttattcgg ttgaaatcgt atgaattggc agatcactta tattgcagac gaaaatctca
    10561 tctatgtgaa aaccagcgga gttgtaacct ctgaggctgc caattcgatg ttgaaggaaa
    10621 ttgttgaggt ggcggtttgt catcaatgtg accgccagat cattgaccac agtggtgctg
    10681 actttgcttt gaggctgttc gagttgtatg agcgtccttc gatcaatgtg cgtatcgggc
    10741 tctcgcacaa atggaagatc gccatgattt tcagcgaact tactcaaaac acctttttta
    10801 tggaaaccgt tttcaggaat cgcggctata acttccgcca atttgataat ctggaaaagg
    10861 ccagaacttg gattttgagt gaataaaaaa tgattcactc taaacgtaaa gaatcagcca
    10921 cgaattgcgc ggaacgtcgc gaatttttca aaagtctgaa agattctacg gatacgcaga
    10981 gaaaataatt ataaactccg tccgcaggag gctccgtggt tcaaaatggc ctttcaaaga
    11041 cattccttgc aacttatgca agatacggac gagatataaa aaagccgacc cagtaaaatg
    11101 ggtcggcttt ttttgcgata ttttttacgt gctggtgcgg gccagccttc tcatagccac
    11161 catttgacga aggtgatcca cttcgtgggc cagcatcaag aggatccagg tttggtagtt
    11221 gcatatccca aaagcagaac tgatttcagt cttttgtggg tcatgggcgt ggcgggtttg
    11281 ttcaatggtc gcgtggattc gttccgtggc ggtggcgaga ctcacgcgca ggtcattggc
    11341 tgaaacattt tgccgtgcgc cttcgccgaa tccacgggcg gcttccacca tatgtggaag
    11401 ctggctggtg gaaatctcca gcaatttgtt gtgataaaaa ccctgggtgt agataagatg
    11461 ggtgatcgat tcactcagag accaaagcga atgggcttgt gaggtgtagg cgacctgggc
    11521 atcggtcagc ggagcgaggt tttcctcggt tcgttcgcgg gtgtagtaaa aggtttcgat
    11581 gagctcatca agcgaaaatc tcgaggccgc ctccagccac gtgatctcct tggtaaaggc
    11641 ggagaggatg aggggattta atacttcaga agtgatcatg gttaaaagac gctgtatctt
    11701 tctgcgatgt tacccagtct cacaggagta caggatggtt tccatttcct gcggggtaag
    11761 tccgccgtga tggccgtaaa aattctgttc gaacttatcc ttttcatacc accagacaga
    11821 ctcatagcgg taagacagaa tcaccagatt cccaacacgt tccaaaaaac ggggggatat
    11881 ttctttgcca aaatagccat ctgcaatcag ggcttctgtt ttaactacat cggccctgcc
    11941 ttccaatctt ctggcaagaa agtcttgtgc ttcatccaga aacccatcct taatgtagag
    12001 gaacatgtca cgggccgagc cagcagggac gatcagccgg tctttgcgat tcttcctgat
    12061 caggcgttga atgccgtcaa attcggggtg ggtgttgagg aagatcgtgg tctttgggtc
    12121 gatctcgcac atgccgtggt cggcagtcat caagaacagg attctctttt tgccattaaa
    12181 gatggtttcg aaataatgct ccatcattaa tagaaaggtt tggatctctg cttcggtttg
    12241 cggggcgttg ggaccatatt catgggccag tgcatcgatc ttgtcaaaat acaggtggat
    12301 gtaggtgggg ttctgttgtt tttccaaaag cagaccaacg tttacgagcg cctctgaaag
    12361 cgttttgaac gcatgcggct ccgagccttc cattactaca ctggaataag tggagggagt
    12421 gtaatcacgc aggccaaagt tgaaactttc cacacccatt ttggcaagtt cgggatagag
    12481 gatgcctttt ggataaatgc ttgcgggatt cacttttttg tttttcagat tgtcgcgatc
    12541 cctggtgcct gcaaaggaaa acaacagcgg ggtgatgatg tggtccacct gcggttcgta
    12601 gtactgccat tcgaatacgc cactttctcc gacgggcagg cctgtatgca tggttgtgac
    12661 atgtgcggca gtggtggacg gaaattgaga ggtcagtttt tcgatcttcc ctgttctctc
    12721 gatgcgtttg ataaaagggg catcttgaaa ccgttcgtaa aaacgccagc cgaaggcatc
    12781 aataaagaac agtaccactg catcgtattt cttcgacgcg aatgcctcaa tgatccgatt
    12841 gggtgtccct gcaaaaccgc ctgaatcata acgaggtttg acgaaagttt tgttcattga
    12901 cctgcctttc gtgagtgtgt ttccattgaa tggatgttta ttcaaaaata aatttggtcg
    12961 ggttctcgaa gaactttgtc catgccaggc attggcgcgg tgtgaagatg tataaaccgc
    13021 cttcatccca ttgatcgggt ttcggggcgt atccgtcgga ggcgtatttg gcgcagtaga
    13081 ctgctgatag tcgctgggcc agttccaggt caggtttgga agcgggggtt gaagtccctt
    13141 ccatgatgac cacgtcattt ccgctttcaa ggtgcagggt cgtgttcgga ttcttttcca
    13201 aattacgggc atgtcgcgtt tcagggctgc catcgtaata caacttgcca tcgaggaagg
    13261 cgccccaacg cggaacaaca tgcggacggc catcagggta cacggtgcaa agccaatagt
    13321 tgatcgactc ggtcaaccgt ttttcaacat gttcccatgg cacctcggcg atgggatgat
    13381 ctacatagcc ttttggaagt ttggggcggg agatcgttgg catgtttatt ctcctaataa
    13441 atagcggtcg aagaatgctg cgaccctgtt ttcatattcg aatgtgcggc gcgtgtcgaa
    13501 ttccacgtgg ccgagttttt catcaaacca cagttctttg ggttcgcctg cggcattgta
    13561 gagacgttcg ccgctgacgg gagaaatgac ggtgtcacgc ccgccttgca tcaaaaatac
    13621 cgggcgcgga ctgatgagcg gaatccattc ggtggcatta atctcctcga tcttaatacc
    13681 cgtttgctgt tcggcccaaa agacgatcag cggcacgaaa ggaaattctg acaaccccgt
    13741 gaaatgcttg acgctggttg ccaccgtatc ctgcatggac gagaaagcgg aattcgccac
    13801 cacggctttg atgttttcgt tttgtgatgc aaattgaatg gccaacatac cgccatagga
    13861 attgccgagg atgccgatct tttcggggtc gatttcagtg cgggtgagca ggtattgata
    13921 ccaggcttcc atatcctgca cttcatacgc gccaagcgtg atcaattcac cttcactttc
    13981 atcgtgagcg cggatggatg tgacgagtac attgtagccg tggcggtgca tcatttcggc
    14041 ttcgttcaat aattcagagc gtgtggattt gtagccatgc tgcatgataa tgaccgcatc
    14101 attctcgccg gggatgaacc agcccgccaa ctccatgtca tcggggttgg ttacggtcac
    14161 agattcgaat tcgagtttaa gatcatcggg ggtgtcgatg ggcagtttgc gggtttccat
    14221 gggggagtgg agcaggttgt aggcttcgct gcgggtcttc aacaacagcc ctgccgcaac
    14281 aacgaccaac agtcccaaaa tgacacccaa aacgattttt gtaattcgat tcattctctc
    14341 tccgtaattt attgaaatgc tgatccgcta ttttcccaag gcttgtttca cggctgcgta
    14401 cacatccact acgccatagc caaaggcgtt gctggggttg ctccccgtaa aacaaccgct
    14461 ggagatgtta cctgtatatg gtttggctgt atcaatgata agctgctcgg ttgcgtcaat
    14521 atcaccgatc aattcaggtt gagccgacca gatcaacgcc accacgccca cgatatgcgg
    14581 gcctgccatt gaagtgccgc tgaattcggc ataatacccg ccagggatgg aggataggat
    14641 gtttacaccc ggggcaacaa tatcgggttt ggtgcgcccc gaaccatccg cagtgacagg
    14701 gccgcggctg ctgaaatcgg ccatgttgcc gagaatgtca atggcgccga ccgaaaagac
    14761 cgaatcgtac aacgagagcg gcgattcaac tgtttcgcag tttgggccat cgttgcccgt
    14821 ggaaaccacc acgaaaatgc ccgcatctcg taaattgttc gccgcataca acaatgcatt
    14881 cgggtcacag ccttcgatgg atgggcaccc ccaggaatta ttcagcacat gtgcggcttt
    14941 ggttgggtca ccatctttca acgggtcacc gccatgcggg aagggtgcca gcatgaactg
    15001 catacaatcg agatacaagg cggggttggc aaggttgcgg tccaaattca cacaaccgat
    15061 ccattgcgca ccgggcgcca cgccgatccc attcacgccg aggatcgtgc ctgtggtgtg
    15121 tgtgccatga ccgccttcat cattgggtga ggtggtgcca tcccacgggt caaaccagtt
    15181 gtaatcatcg ccgctgttaa aaccgcgata ctgttcacgg atggcggggt gattcccatc
    15241 cacccccgaa tcagactggc cgaccacaat gccttcacca gtgactccaa attcgttcca
    15301 cacttgatcg gcgccgatca tggcgatatt ccactgcgcg ttcgattcca catccatggc
    15361 accttcagac gccgcagcat cttcgggagc agcacgcagg cgcgggctgg ggatgactcg
    15421 gtccacttcg gggcgggtca tcaaatacag gcgcaccaaa gttccgccgc gtacctccat
    15481 cgcattttcc agataatacg gtgtgtattc gatgcctgcc ttgtcgaaaa aggcacgcaa
    15541 ttcagattgc gtttcgttcg cgtgtgcggt caagcgggta taggcttcgg tcaggcgttc
    15601 atcgcggtct ttaatttttg ccacgtcaga aagatcagcc tgatctttga ggatgacgaa
    15661 aagacggtcg ccataacttc caggattgcc aaagatgaaa aatacagcga cgacgagaat
    15721 ccatgcaact ccagcccctg cccaaccgat ggcgcgcttc gttacggacc gtgagctggt
    15781 ggctgttaca aaacgcagaa tcagcgcgac gatgctgacg ccccaaccga tgaccattac
    15841 ccatccgcct gcactcattg ccagccctcc aatatcaccg aggacaaagg tcaactcagt
    15901 gggatcgaac atggcaagcg tgacaaaggt gagcatggac accgcggcgc ctgccgcggc
    15961 tctggaaggc atgactgcag caaccgcaaa cgcgaaggac ggcagagccg ccagcaaaag
    16021 caattgtgct ccgtcatacc cgagggaaga tccaagcaag gctaaaaccg caccgattcc
    16081 aattccatcc agaaaaacat tttctgtgct tgattcgatc aagacagccg ccagcaaacc
    16141 aaatgcagca cttgcgaata gactcaagac aacatcacct gccagcccaa gcgagccata
    16201 gatggcaaac ggcaatgcac caatgccagc caccaccaaa ccgaatggca gggaggcact
    16261 atcccatttg agtttgttct tgcggatcaa aatgacaacg cccgtgccaa ttccgcacaa
    16321 aagcatttgt gcgatgaacc ccaattggtc gttgttgggg ccgatcgcac gcagagccag
    16381 ggctgggaaa cccatcagag atgcaccgag caagcctgca tatacaggtt tgaagcggtc
    16441 gtctttggtg aagtaccaca gaagggcggt caaaatgctc atcaaaatgg cttgtcccaa
    16501 cagaccaaga ggtcctgccc aggcaaagtc ttttgcactt gatgaaatga tggcgctttg
    16561 ttcggtggac cacaaaacga agtgataaat gaacagacag aatggcatgg gcagcgcaaa
    16621 gaacaggaac aggatcaatg cgccagtgcc gcgattctgc ggttcaggag tttgttcgac
    16681 gggaggggtt tcttcagtca tggaattctc ctttttgata aggtcagagt gtggattttt
    16741 tgtccgcaga tggttgaccg cggttaaagt gtaggcgggt ttcgcgccaa atcaagtcaa
    16801 ctgtggaagg tgggttatta aattattttt gtgcagggtg tggttaatct tctggtgcct
    16861 gcggaacgag tgcctcgggg ccatgaggtt gcacatggaa aattttgaat ccccattgca
    16921 ccgagggccc aaccaacagg gcatacacca atgtgccgat cccaacgggg ccgcccaaca
    16981 accagccaat ggttaccaca gtcacttcga tgatggcacg tgccacgcgg atgctgatcc
    17041 ctgtggtgcg tttgatcgca agcatcatgc tatcccgcgg acctgcaccc gcatctacgc
    17101 cgatgtagat ggcgcttgca attcccatta acaggatggc cagcagcagc attccaccct
    17161 gtaaaggcag gttgtcttta acggagggaa tgatggagag ccaaaggtct tcccaggggc
    17221 cgatggataa aatgttcccc aatgtgcccc agccgatctt ttcacgcatg atcaacgcgc
    17281 ctgaaagcac aataaagccc atcatcacag tcattgtgcc aatggaaatg ccgattttgt
    17341 gggccagagc cacttccaac acggcccagg cgcttgtgcc gaggttgccc tgaatcatca
    17401 agacaatggc aaggccaaac aacatgaagc cgatctcgat acgaataaaa tcacggaaaa
    17461 acgtattcca tcgtacgggt ttaaggatgt ttttcaaaaa ttgtctcatt tcaagatgat
    17521 accatgatgt gatagactcg ccgcatgaca cagattattg aagttcaacg tgagcaattc
    17581 accatcagca ctgaccctgc gcgcttgaac cttgaaacca tcctcgattt tttcacccgc
    17641 gcctactgga cacaaggccg cccgcctgag cgtacgaaac gggcgatcga aaattcactg
    17701 gtttttggcg tgtacgaagg tacgaagcag atcggcattg cccgcgtcgt cagtgatctt
    17761 tccatttttg cctatctgtg cgatgtattt attcatgaag attaccgtgc ccatgggctc
    17821 ggcaaatgga tgcttgagac catcatggct caccccgacc tgcaaggcat ccgccgctgg
    17881 actttggtga cacgcgatgc gcacgggttg tacaagcaat acggctttga tgcactcacg
    17941 aaccccgatg gctggatgga aatgatccgc ccgtttccgg gagagtagca gggatgcgga
    18001 tcttttggtc cgtgtggaaa agagcaatga aaatcccgca atacaggctg tattgctttt
    18061 ggtacaatcc tgcccatgaa aataaaaaac tggcaattct ggcttggggt gttgatcagc
    18121 gtcatcttta tatggctttc cctgcgtgga cttaaactca atgaattttg gggcacggtt
    18181 caaaaggcaa attacctctg gttgatcccg gggatcggcg tgtattttgt cggcgtttgg
    18241 gttcgggcct ggcgctggca ttacctattg aaacccatca aggaaatccc cacgaaaacc
    18301 atgttccctg tcacaaccat tgggtacatg gggaacaata tctaccccgc acgcgcaggc
    18361 gaagttttgc gcgctgtgat cttgaaacgc aaggaaggcg tccctgtttc tgcgtcattg
    18421 gcgaccatca ttgtagagcg catttttgat ggggtggtca tgctggcgtt cgtttttgtc
    18481 aatttatctg agctggccaa attgacgggt gcttcgggtt tcgtgggcaa tattcaacaa
    18541 gtggcgatca tcggaacggg ggttttcctt ggcgcgttga ttgtcttttt gctggcggcg
    18601 atgtttccgc acaccacggc aaagatcggt ttgtggttga tcgaacacct gaccccaaag
    18661 cgactgcata cgaagatcat cggcatcatg aacaaatttc tggatggact tgcttcgctg
    18721 cggtctcctc ttaacgtgtt gatggtcttc ctgacctcgg tcatcatctg gcttttggaa
    18781 acgggcaagt actggttcgt aatgcatgcg ttcagtttca acgtttcatt ttttgcgttg
    18841 atgttgatga atggtattgt gaatcttgcc accaccattc cctcagcccc gggatacatc
    18901 ggcacatttg atgcgcccgg cattgccgta ttgaccgcct atggcgtgga tcaagccact
    18961 gcagccgggt acaccctcac cctgcatgtc gcgctctggc tgcccatcac cattttgggc
    19021 gcttattatc tggcgcgtga aggcatcaaa tggtcggata ctctgcgcga agaaacggaa
    19081 aatacggatt gataaaatga caacccgtat ttacgttgac tggtctggag acccgggatt
    19141 taaatttcgc caaggttcgt cagaactgct cgttattgca gctgttatgg cggatgaaga
    19201 acccaatata ggaaaccttc gtgaaaaatt atctcttccg gataattatg aatttcattt
    19261 ttcaaaagcc gatcatcgta tccgtgagca ttttaggaat tatctcaata ccgaattgga
    19321 aatacccggt gtggttgtgc ttcgggccga taagcaaatg ttttcattga atatgcgtca
    19381 gaaaaggggg gaacaagtcg ttgctgactt gattgcccaa tgtgttgtta atttgccagc
    19441 cgaattactg catgactcta ctttatacta tgatggagag aaagaacaag aatctttcaa
    19501 gaacacttta cgggtaacac tatcccaaac gcttcattct ggagtgtttt tgcgtagtgt
    19561 aaaagctgtg cctgccagtc ggaatgatgg tttacaggtg gccgacatgc ttgccgggtt
    19621 tgttcgtaat aatccttcag cgataaaatc aagtattgtc aaactgatcc attaccccga
    19681 ttaaatcaaa agcgcctggc tagttggggt tgcttttata caggctgact tacctcacct
    19741 gaaacccaac ccgcgcgatt gcgcgactac accaggctgc tgggttcata ttacaccttc
    19801 aagaaacgag agtcaaggaa tgaaaatagc aattgtcgga gcaggatacg gcggcatggt
    19861 tgccgcatgg gatttgaaaa aagcgggtca cgatgtgacc atttttgaat cggccgaata
    19921 tgcaggcgga cttgccagcg gtttcaagga accccactgg gattggtcag tggagaagtt
    19981 ctatcaccac tggtttcaat cagatggcga aatgcttggg ttgatccgcg agttgggctt
    20041 ggaagacaag gtccgttttc cgcgcccgtt gacggtgatg tactacaagg gcaaatggta
    20101 tccctttgac tcgatcctca atgcgctgct tttcccgggg ctgggttggg gcctgaacaa
    20161 gatccgtttt ggctttgtcg ggctgttcct gcgcctcacg aacaattggc gcgcattgga
    20221 aaaggtcacc gcggatgagt ggatgatgaa atatgcgggc aggcaggtct acgagcagat
    20281 gtggaaaccg ctgcttgtcg gaaaattcgc gtccttttat aaagatgtca acatggcgtg
    20341 gttgtgggcg aggatgcatg cacgcaccac gcgtcttggt acgttcgaag gcgggtttca
    20401 aaagtttgcc gatttgttcg cggagaaact gcgcgcacag ggtgtgcaga tcaaacttgg
    20461 ggtgagcatc aaatcaatta aacgggatca ggcttcaggg agtctgctcg tggacgatca
    20521 gcctttcgac aaggtgctgg ttacatcgtc gccgagcctg atggcaaagt tatgtccgga
    20581 gctccccgaa agttatttga agggcttgct ggggttgaag tccatgggcg cggtggtgat
    20641 gacgctctcg cttgcgcacc cgctttcaaa ggaaggctac tactggttca acgtgccgaa
    20701 ggaggagggc tatccgtttt tggcgctggt ggaacatacc aattttgtgc cgaaggaaaa
    20761 tttcggcggc gatcatattg tgtatgcagg cgattacctc gagcttggac atgaatattt
    20821 ttcaatgagc gatgagcagc tgctcgaaag attcatccct gcgtttcaaa aattcaaccc
    20881 tgagttctcc cgtgattggg tgaagaagat ttgggtccac aaaaccaact atgcccagcc
    20941 tgtgccattg gtggatcatt caaagaacat tcccgagatc cagacaccga tcgaagggtt
    21001 gtatttcgct tcgatgagcc aggtgtatcc gtgggatcgc ggcacgaact ttgcggtgga
    21061 gatcggccgc aaagctgcgc ggatgatgaa gtaatctccc ataccgcaga cttcagtctg
    21121 cgagttaaat ataaaacaca gactgaagtc tgtggtactt aaaatagaca aggcaatact
    21181 cttcatgtgg ttctatcttc ttcaaggcat tgggttcgga ttcgccgccg catcacagcc
    21241 tgggccgttt caaacctatt tgatctcaca gaccctcaca cgcggctgga aacacaccct
    21301 gcccgctgca ctggctccgc tcgtcagtga tgggccgatc attttgctgt gcgtgttggt
    21361 gctcagccag gtgcctgcat ggatgcagcg cgggttgtac attgcaggcg gcttgttcat
    21421 tatttatctt gcctacggca cgtatcgaac atgggagaat tttgacactg cggcgcaaac
    21481 caccgctccc gcaggcgaag ttcaacaaag cgttctcaaa gccgcgatga tgaatgcgct
    21541 caatcccaac ccatatattt tctggacgct ggtcacaggt cccattttgt tgagtggatg
    21601 gcgtcaatca ccgatccttg gtgttggttt tctggccgcg ttctacctca ccatgatcgc
    21661 agggctggcg gcagtgatcc ttatttttgg gttcgcacaa aggttcggcg ccaaattcaa
    21721 ccgcatgatg ctgggcattt cagcgatcgc cttgttttgt tttggcattt atcaattgtg
    21781 gctgggtttg tttccaaagg agatcctatg aatatcgttc ttgtccatgt gcatgtcaaa
    21841 cctgagtttg ttgaagcctt taaacaggcg agcattgaaa atgcatcgaa cagcataaaa
    21901 gaggagggga tcgcccgctt cgatgtcatt cagcaaaacg atgacccgac ccgcttcatt
    21961 cttgttgagg tgtataaaac cgccgaggcc tctgcggcgc acaaggaaac cgcgcactat
    22021 gccaagtggc gcgacacggt tgcagagatg atggccgagc cgcggcaggg gatcaagtac
    22081 acgaacatcc atcctgaaga ctctggctgg taatttatgc ctgcatttga atttgccacc
    22141 gcaaaccgag tcatcttcgg ggcggggaaa ttaagtgaac ttggcaatca ggtcaagggg
    22201 cgtgcgaagc gtctgctttt ggtgcgggga aattcatcgg atgcgattcc gcgtgtgaag
    22261 gagattctct ccgggcaaga catcctcttt aatgaatttg cgcttcacgg cgagccgaca
    22321 gttgccacgg cctccgaggg agttgacctt gcgcttcaac atggtgtgga catggtcgtt
    22381 gcacttggcg gcggcagtgt gcttgatgca ggcaaagcca tttcagcgtt gacgacgaat
    22441 cgcggcgacg tttttgatta tcttgaagtt atcggcaaag gacagccgct ggtgaatgcg
    22501 ccgctgccgt tcatcgccat cccaaccacg gcagggacgg ggactgaagt gacgcgcaat
    22561 gcagtgcttg gctcgcctgc tcatggggtg aaagtgagtt tacgcagccc gatgatgctg
    22621 ccgtcgcttg cgcttgttga ccccgcgttg acatatgctc tgccgcctgc gatcaccgcg
    22681 agttctggtt tggatgcgtt gacacaattg atcgagccat ttgtttctgt gaaagccaac
    22741 ccgatgacgg atgcgatttg ccgcgaaggg attcgtcgtg cggcaaggtc attgcggcat
    22801 gcctggcaca acggggcaga ggttgcggcg cgtgaagata tgtcgctggc gagtttgttt
    22861 ggcggcatgg cgctggcaaa tgccgcgctg ggtgccgtgc atggattcgc gggtccgctg
    22921 ggcgggatgt tgaatgcacc ccatggcgcg atctgtgcaa agttgttgcc gctggtgatg
    22981 gaggcgaatc tcaaggcgtt ggagagccgt tacgcaggcc atgtggcgat cgagcgatac
    23041 accgaaattg cacggatggt cacagggaat gaatctgctt ctgcctggga tggtgtgcag
    23101 tgggtgagtg atctggtgcg cgagttgaag attccgccgc tttcgacgca tggaatgaat
    23161 gcggctcaca ttcccgaagc cgttcaaaaa accttgaatg cgagcagttt caaagggaat
    23221 ccgattccgc tgaatgaggg ggagttgcgc gagattttgg agaaggcgtt gtagggcctt
    23281 gaagttcaca tgctcctcat gctataaaga atatgtagga aaggaggagc catgtgtaat
    23341 ggtcacaatc acggggctgg ttgttcgtgc ggatttggtc cgccgtatct tggtcccgga
    23401 aaacccattc caaagggcgg gcggggtgat cattttaggg gtgttattcg agaacggaag
    23461 cgtgagaact gggcagccgt gggggcaata aataaggaaa agatcgaaca gagtttgggg
    23521 caattgagat tgggtgcgag atggaggaag gccattttgg agcgttactc caaggctgac
    23581 tttccgattg catcatctgt gtgggaggga tatagtgaag gccgcaaacg gggtgcagcg
    23641 cagagaatgc tgcgggctgt ggggctgcgt cagaagatcg ttgaagagtt ggagcccatc
    23701 catcttgaaa ttccgttgtt ccgtcttcag ccgccccgca cggaaaatag caaagtcatt
    23761 tatcaggaaa ggcagatcaa agaacgtggc tgggatattt cggtcaaggt gcctgggttt
    23821 gccaccggtg cagatttatc catgtacctg gaagggaaag ggttggtgga aacctctcac
    23881 aaggaatgca agcaaattgc cttcccggtg accatcaggc gttatcgggt tgatctgttt
    23941 ttaggcaatc tctgcattgc aaggaataaa ctgatcgcag aagcggggaa tcagaaaacg
    24001 ggtcagattt tcagccgtac gattatcgcc tgtaaaaaat tcatcccgcc ttcgccccag
    24061 tcggaaatga tcgttgaata tgatctttcg aaggatgaat gcggtaattc aaaattcttc
    24121 ctcggctggg gggaaaagat gcggcgcaat gcggaagtta cgatcccgat tccgggaatt
    24181 ccttcgggga tacaggtctc aatttcaatt caaagtgata ttcagcttga gtttgacctt
    24241 gagcccaaga ttgattataa actttacccc accccggaag gcatcggtat tagctgggag
    24301 gtgagcagag gaaaagcgaa aagaaaaaat atcaggaacg acaaaggcat ttcacaattt
    24361 aagcagagac cgaagaaact tatctatgtg aagagacatg tgtaatgccg cgattcttta
    24421 tttgaccatt tacataagtt gtaaggaatg tctttgaaag gccattttga accacgaaga
    24481 cactgaaaca ctgtaattgt ttcctctgtg tctccgtggt taaactttca gactaacatg
    24541 gaatgcaaat cgaaaagcat gtagagacca cgggtttttc cccggcgagc gatcgtctgc
    24601 attttgtttg gcttgtaaaa caaactcacc ccgaaaagct aacctttcgt gggtgagttt
    24661 gttttgaatc aggcgtcgag aatcggttta cccgccagtt aatgattttc tttcctcaac
    24721 cattttcaga atgagatcgt ggatctccgt caaacggaag ggcttggtga tgtattcatc
    24781 ggcgcccgct tcaatgcagc gttcgcggtc gccgggcatg gccagagcgg tgatcgccac
    24841 cacgggaaca gaggccagcc gtcggtctga caggcggcgc aaacggcggg tggtttcgag
    24901 cccatccata ccgggcattt gaatatccat caagacaatg tctgggtgga tatgcggagc
    24961 ctgggccagg aaatccactc cgctttttac ggagatgata ttgaaattct gcatggcaag
    25021 aaaatcggcc aatgtgttga tgttgacctc gttatcgtcc acgatcatga tggtggcaac
    25081 ggttgctgcg gcagggttga tgtggccttc actgctggcg aggctcggtt tttgaatatg
    25141 acttagggcg gtgcgcaggt ctgaaagagt gaagaacttg acgaggtatc catctgcgtc
    25201 caacttggaa gccttggatt tgtcatcatc cacggatgtg ataacgacgg gaatgtgttt
    25261 ggtgttcctg ctgttcttga tctgggtgag cacatcccaa cctgaaactt cgggcaggtt
    25321 gagatcaagg aaaacaacat caggctgtgt ttcaatgact cgctccacag cgtccttgcc
    25381 gctggtgtgc atgatggcgg taatgcccag cgacttgacg taacgtgcca ggtgttcagc
    25441 gtcggcggga gtatcttcga tgatcaaggc ttgatggaat tttagttgag acggattttc
    25501 aacgtcctgc gaagccttgt ccgcaggctg gcagggcagg gtcactgtaa agcggctgcc
    25561 ctctccgggg acactttgaa cgtcaatgct tccaccgtgc aattcaacca gtcgttgtac
    25621 caaagccagc ccaaggccgg tgccgctttg ctgccttgaa aggctgctat ccaattgtac
    25681 aaatggctgg aatagttttt tcaggtcttc gggggtgatg ccaatgcctt tatcccaaat
    25741 ggagatgtat accaagcctt cactatcatt ggctgtgaca tccaacccaa gcatgccttc
    25801 ttccggggtg aatttgaccg aattgctgag caggttgacg aggatctgct tcaatcgccg
    25861 cgcatcacca cggacactga tattggccgg gttcattgta aagttaacag tctgacgctt
    25921 tttgcctgcc atgcctttgg taagctggat gctgctctgg cagatttcgc tgagcgaaca
    25981 ggcttccatt tgcaattcga gcttgcccgc ttcgatcttg gatacatcca gaatgtcgtt
    26041 gataaggtcg agcaggtgac gtccgctggc ttcgatattt gcaagcgtgt tttgctgctt
    26101 ttcgttgatc ggaccgtaca cattgatctg cagagcttcc gaaagaccca gaataccggt
    26161 caatggggtg cgcagttcat ggctcatgct ggcaaggaat tcatctttca tgcgcatggc
    26221 gcgtgccagt tcgaggttgg cattgcgcag ggcctgttca gccgcttttt gaccggtgtt
    26281 atcgaatacg gaggtgcggc tcatcacaaa ttcgcctgct tcattgtaga ctgccgtagc
    26341 gctgatcaat accgggacga tgctcccatc tttgcggcga aattccgatt cgacatcgcg
    26401 taccatgcca tttttcaaaa aggacggata gatctcttta aacccttcca gacctcttgt
    26461 cgtaaggaat tccgtgatcg aatggccgat catttcttcg cgggtgtacc ccagccaggt
    26521 gaggtgggtt tggttgataa acaggagtgt gccatctgca tccagtgagt ggtagccgat
    26581 gggtgagttg tcgtacagat cttgcacttc ggcagtgcgt tgttttacgc gttcttcaag
    26641 tgtgcggttg aggtcttgca actcctgctg ggcggttttc aggttgtgaa tgtcggttga
    26701 gttgatcagc acctgcgtaa ccagcccgtc ttcgtcatgg atcggtaaaa tggaggtgcg
    26761 gtaccagcgg ggcgcagttg aagaaaaact gggggcttca aatgtaatgc cttgatcacc
    26821 atggattacg cgttggatgt cgctcatttg tctggctgtt acttctggcg gaaagagttc
    26881 atacatggtc ttgccaatta attcatttgg cgtcaggtgc atttgcgcgg cagccatatc
    26941 gttaacatac aggaatctgc catcgttatc cacgctggcg atcatattat ccagactctc
    27001 gatcaacaca cggtattttt cttcactggc atgcagggca tcttccgctt ttttgcgctc
    27061 ggtaatatcc tgcacggctc catatacccc tgttaagcat tgaagttctg aatcccagag
    27121 cgggttggca tggacttgta cccagcgtat atcgccgttt tttgcgatca ttctgagaac
    27181 actgacgatt ttttggttct tttttaatgc agcaaaatcg gcggcatcct tttctcggtc
    27241 ttcggggtgg agaatggaga gccaacctcc ctgttttgaa tattcttcaa aggtgtaccc
    27301 ggtaatggct tcaaagccgc cgcccaccca cttggtggat gttttgccat tcatatccac
    27361 atcttctgaa aatacataat ctgcattgat cgtggagatc atgcggtaca gttcttcgcg
    27421 ggcgcgcaag gcttcttcag tttgttttct ttcggtgata tccagatgag taccgatcat
    27481 gcgcagcgga cgctgttccg aatcccattc catgaccttg ccgcggtcaa atacccaaac
    27541 ccatgaaccg tttttgtgtt tcatacgaac ttcacattcg taaaactggg tttcaccagc
    27601 aaagtgcttt ttcaacaaac catccgatat ttccaaatca tcaggatgac agaaatcagc
    27661 ccaggtttga atgctgaccg gttcaatttc ctccagcgca tacccaatga tctcggccca
    27721 acgttcattg aatatggttt cccctgtttg cacattccaa tcccacatgc ctgcatttgc
    27781 gcctttcaga gcgagctcca cccttgattg ggtctcacgc aggttttgtt caacctgttt
    27841 tcgttcagta atatcgcgga caatggccaa tacctcatcg gttccgctgg gggtcatgcg
    27901 ggcttcgtaa tcacgcaggc cttttccggg gacgggcaac tggtattcaa atgtctgtaa
    27961 ttggttggct tccagagtct tggctatgta atgatcgatc aggtcggcga attcaacagg
    28021 cgcgatatct cgatttctgg ttccgatcag ggaaacggtt tgctcatgca ggtcttttgc
    28081 atctgccttg tagtctaaaa atacgccttc acgattcaaa cggaacatca gatcggggat
    28141 ggcttgcagc atggcctgtg cgcggccttc actttcccgc agggcatgtt cggctttttt
    28201 gcggtcagta atgtcgcgtc catacaccgc cacccggcga attgtgccat caccatcaat
    28261 aattgggtag atgctgtttt ccagccagac gccgaaccgt tcatcttcaa atatgaccgg
    28321 cttgcgcttc tcgatgacca tttccacctg cttctttctc gatgctccga cgctttctgg
    28381 aattagatca tacatgtttg tgccagccag atcagatact tgtttgccga ggcgggcggc
    28441 agtggtttca ttggtggcga tcacatttcc actggtatcc attaaaaaca cagactcagt
    28501 ggaggcattc aagatcgcct gagaggtttc attgcttgct cgcaacgcgt cttcagcctg
    28561 tgtacgctca tttagttctt ttagcatctg gttgtacaga atcgcatttt gctctgcgga
    28621 tgcgatatgc aaggccagag attccaggag cttcaactgg ttttccgtat aggcatcctg
    28681 acgataactc gatacctgaa tgatcccgcg gacgatccca tcggatttca gtggaacgat
    28741 caaagccgaa cgtgtaactt cttcttcagg tgtttcatca tcttctgatg agatttcatt
    28801 ggtgtcgctg tctacctgat aaaccgtact tgtatttttc atatacgatt gaaaatcatt
    28861 gagaagcatg gctttgcccg tgcgtatgac gaggctctgt gtgcctttgc cttcctcttc
    28921 aagtggaatg gacgggaaat tgctgacatc cagccatttg ttttcgatcc agtaggcgcg
    28981 gcaggtgatt aactgtgttt cctgatcgaa tgctgaaatg aacatggtgc ttgtgggaat
    29041 ggtggtcacc atgaagtcac agatcgtctg atagatttcc tgcaggttga gggtacgatt
    29101 tagcttttgg ctcgcctcat acaacaggtg aatctgctcg ctttgctgac gggttatctc
    29161 ctctgacagt ttgcgctcgg tcacatcgcg gaagttgatg acgatgccgc tgatacttgg
    29221 ctcgtcgaac atgttggtat aggtgctttc aagccagtga tactcaccgt tcttgtgcat
    29281 gaagcgatat tccacggttt gggggacatt tgggttggaa agtagttttc cgcgcaggtt
    29341 tgccagcaaa ctcacatctt gtgggtggac cttttcacgc gattgtgtgc ccacgatctc
    29401 tgccgcgtca tacccaaaca tgcggtatgc cgacgggctg gcaaacaaga acattccatc
    29461 tgcgtccacg agggcaatac cgtccggggc atgttcgatc agggcggtgt tacgtttttc
    29521 gatcaagcga atggcggctt ccgctttttt acgctcggta atgttgcgtg agacgccata
    29581 cacctccaac cggttggtct cagggtcgat tacataacgt gagatgacct ctgtccagac
    29641 ggtggagcca tctttacagg tttgttcgat ctcatcggta taggatttgg aaagaccctg
    29701 ttggaatttc cccagtcttt ccggagtgac ttgagaaaga tattccaatg aaacaggagt
    29761 gagagcggcg gccatatcct ccgtcatgac ttcctccaca ctataaccgc gtaaagaagt
    29821 gacagatgga ctgacataac gaaaacgtga tgactccacg tccaaaaccc agatcacatc
    29881 tgaaatgttt tcagccagaa ggcggtactt ggcttcactt tcacgcagtg cctcctcggc
    29941 ttttttacga tccgtgattt ccatgcccat gcattggatc tcgctgggtt gattgtgttc
    30001 atcggtaatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna agtgggtttg
    30121 tgacgcgatc agcgatttaa gttttttctc gctggccagg agtgaatttt ccgcgagctt
    30181 gcgttcggta atatcttcaa aggttgcgta gacctgatgc ggtttttctg aatttccgtt
    30241 gaagcgcggc gttgcattga tattgatcca gttgtaggaa tcaaaggctg ggttgtacac
    30301 ccccatggtc acattgtgaa tgggctttcc tgtttttagg gcaaccatgg acgggtgggt
    30361 atctcccggg aaatcagaac cgtcttcatg aatggatttc cagcgcgggt caatggaggt
    30421 gcgtccctgc atttggtcca gtgtcaatcc taatattctt tccgcggccg ggttcgcgtg
    30481 gatgatgcct ccgttttcat cctggaaaac cacgccttgt accatcgttt cgaacaacaa
    30541 acgatatttc tcttcacttt cctgtatctt ttcaaaggct tgtcggatct cggtgatgtc
    30601 aagaaccgtg ccggaggcat gtatggtccg tccttccgga tccaatatgc cttgaatggt
    30661 gactttgtag tgtcgggtct cccctggaat gggcgtggca cgatattcaa gagtggcaac
    30721 ttgcccgctt tgaaacgcct cttcctgtgc attcaaaagc cgctgacgat catccgggtg
    30781 gaccatttcc atgaattctg cgagttcagg aataccccgg gctgggtcgc gccgaaacag
    30841 gtggaacatt tcctttgacc agaccattcc cttgccccgt ttgatatcaa gttcccaact
    30901 gcccaaattg gctgtagcca gtgccatttc caggctggtc tgactttttt gcagcaggtc
    30961 ggttgccttt ttgaggtgga ttgcggtggc aacctgcgag gaaacaaatt caaggatgcg
    31021 ctgttcccga atcccataag catgagggtc tgaatagttt tgaactgcca taaccccaat
    31081 gattttattg cgggcgatca atggcacccc cagccagacc ttgggttgtg tgccaacgac
    31141 ctcgatgcgc tggccggttt ggttgtagac atacaacagt gattctccgg tgcgcagaac
    31201 atgactcgtc aaccctttgt ccggaggcat ggattctcgt attgggtcct tttcgtccat
    31261 cgagtagacg aaatgcagta aattttcctc ttcatcatgc aaggcgatat agaagttccg
    31321 ggcagccatg acattggata tgtggtgatg gatccccttg aaaaaatcct gcagcgattc
    31381 ggtggtttgt gcggcattgg caatgtgata tacagtttcc tgtaatatgg ccgattgctt
    31441 gcgttctgta atatcctgtg tgacaccgat cgcagaggcg ggtaccccat ggttgtcaaa
    31501 tttgatgctc gctttgacgc gcagccatct tatgtcatcg cctgcaatga tgcgatactc
    31561 aatatcgaag ggcccgccct gtagcgccgc cgaccatgaa acctgtacga attcccggtc
    31621 atcagggtga atgatgttca ttaactcatt cagagaatga ccgcctggtg cccaaccgat
    31681 taactgggca tcctctgatg aagattcaaa gatgcccgtt ttcagatctg ccgtccaact
    31741 tcccaagcga gccatcaatt gagactcatg caggatctgt gtgcttttta atagcgacac
    31801 ttccgcctgc tttttctctg tgatatctcg aatggcgctg aggattacca cctcgttttt
    31861 cattttgtgg tggctcaggc tgatctccac gggaaattcg ctgccatcct tgcgaagtgc
    31921 atagattccc tgttggatgc ccgtggtttt gtgctgcgga ttttgcaaat agtagtcgcg
    31981 tttttggggg tgggcatcac gtaatctttc gggaatcaac tgttcaattt tcatctccag
    32041 caattcgctt tgtttgtagc cgaacagctt ctctgtctgc aggttcgcca gaatgatctt
    32101 cccctgagca ttaacaatga tcatggcatc gggtgcggaa tctaaaaagc ctttcagccg
    32161 ggcctcgctt tcacttaatc gttcttcggc cagttttcgc tctgtgatgt cgttcatcac
    32221 ggtcaataga aaactttggc tattcacctg aatatattct gccgaaaaaa ttccatgcct
    32281 cagccctccc gattttgtgc ggaccaaaac atcttcatct ctcaaaaaac cctgctcttt
    32341 catcttgccc aataaagccc ctctttgatc tgggtcgaaa aaaagattta attcaagggc
    32401 ggtctttccc aatacttctt cacgggtaaa ttccagggtt tccaggaagg tcttattgat
    32461 gtcaagatat ttccccgtgt tgatatcggt gatggccatt tgagccgggc tggcctggaa
    32521 aaaacgggag aaacgttctt cattttcatc aggagcgggt agtttgcgtt ccaattcttc
    32581 gatccgcttt tgcgccaatt ccaattcctg ttgcagttgt aatttggttt tttgcttgct
    32641 catgagaaac tcccgtatga ttcaatggtc cacctttggt tatgtaattt agggcaatga
    32701 aatcgggaag gcggatttgt acaagttgaa aataacgcct gtcattgtaa ttgtccataa
    32761 gtaaaaatac ttaattaacc agtgcgttac ctctgtaggt tcatatagaa aaaacccgcc
    32821 tcatacaagg cgggtttggg tctttcttaa tcacccctga tcattttttc agtttctttt
    32881 ccaactccat tacagtcagt acgatcactc ccgtggatgc agcaatcgcc aggtcgcagc
    32941 cgctcagcgg cattacttcg aagaagtgcg ccagtacggg gatatacatc accatcaatt
    33001 gaagaacgac caccagcaac cccatgcctg ccagcagtga attgctcagc agccccattt
    33061 tgaatactga ttcttttgat gagcgtgatg cgaacgcctg aaagacctgc atgaaagcca
    33121 gcgaagtgaa gatcatggtc tgccattcgg ggcggcctgt gaaaaagtac caggaaccaa
    33181 gcccaagtgc gagcgcaccg atcattgcgc cgacccagat ggtttgcgta cctgctccgc
    33241 gtgagaatac gccttcagtg ggggagtagg gtttgcgctt catcgtatcg ggttcggggt
    33301 tttccacacc catgccgagg ccaagcaggc catcggtgag caggtttagc cagagcagct
    33361 gcaaaggcag caacggaagg ggtttcccaa gaaacggagc gatcaacatc accagcacct
    33421 tgccgatgtt gccagccaca ctgaagcgca cgaatttgcg gatattgtcg tagatggtgc
    33481 ggccttcttt gacagaggca acgatggttg caaaattgtc atcgagcagc acaaggtcag
    33541 aagcctcttt ggaaacatcc gtgccggtga ttcccatggc cacaccgata tctgccttgc
    33601 gcagggcggg cgcatcgttt acgccatcgc cggtcatcga aacaatgtga ccgttttcct
    33661 gcaacgcctg cacgatgcgc agtttgtgtt ctggggcaac acgggcaaat acattgacct
    33721 catccacaac gctccgcaat tcagtggatg aaagattttc gatctcaaca ccggtcaggg
    33781 cgcggccatt ttctgtgatg cccaattgcc gtgcgatctc gatcgcggtc agcggatgat
    33841 cgccggtgat catcaccggg cgaatccctg ccgcacggca ggttgccacg gcttcctgca
    33901 cttcgccgcg cggcggatcg atcatgccaa acagaccgac gaaggtcagg ttttgttcaa
    33961 ggtcggtttg aatgacctca gggatggaat tcatcaaacg gaaggcgatg cccaatacgc
    34021 gcattccttt tttggcgaga cgttcattcg ccgcttcgat cttggacctc caacctgaat
    34081 caaggatttg agattttcct tccgaccaga tatggctggt gatgtccagc aatccatcca
    34141 caccgccttt ggtgaaggca atgtaccgat ggtcgccgat ttccagccct gcaagcacgg
    34201 ttggatcata ttgaccaagg tgatggacgg tggtcatgcg tttgcgttcc gaatcaaagg
    34261 gaagttcggc agcgcgcggg aaggatgggt caagcgaatc tttccagtaa cccattttgg
    34321 cggcggcaac caccagagcg ccttcagtgg ggtcacccaa agtgtggaag cggtcatcgc
    34381 ctgtgtctat caactttgag tcgttacaaa gcgcgccgcc gatcactgcc agcgacagag
    34441 aggattgggc cggggcaccc agcccacggg tggcgcgaag cgttccgtcg cgttcgatct
    34501 cttcggtcag atccagcgca tggtcggcca catcaagcac aacgaccgtc atcctgtttt
    34561 cggtgagggt gcctgtttta tcggaacaaa tgaccgtgac cgagccgagc gtttccacgg
    34621 cgggaagttt gcgaatgagt gcgttgcgct gcaacatgcg ttgtgcgcca agcgcaaggg
    34681 tgatggtgac cacggcgggc aggccttcgg ggacgatggc gactgccaca ctgacggcgg
    34741 tgagcagcat gtgacggagt tcatcgccgc gcagcaaacc gaggatcaag atgaggatgg
    34801 cgatacccgt gccgatgagc gcgagatttt tgccgagcgc atcgagtcgg cgctgcagcg
    34861 gagtgttttc ctgttccact ttttgaatga ggtcggcaat tttgccgagt tcggtctgca
    34921 tgcctgtggc aatgaccagt gcaagaccgc gcccctgtgt aacgatggtg cccatgtacc
    34981 ccatgttgcg gcggtcgccc agcggaagtg cgaggttggg gtcgtttgaa agggcgctgg
    35041 tgtgtttttc gatggcttct gattcgccag tgagcgcggc ttcctgaatc cgcaggttta
    35101 cggcttcgag caggcgcaca tccgcaggga cgacattgcc tgtttcgagt tgaaggatgt
    35161 caccgggtac gagttgattg gcggcaacct ctttcagcga gccgtcgcgc aacacgcgta
    35221 cattcggcac cgacattttt ttgagcgctg cgatggcctg ttcagcgcga tattcctgca
    35281 caaaaccgag gatggcatac agtgccacaa tggcaaggat ggagatggtg tttttgtaat
    35341 cgccgaggat gcccgccagc actgctgcag caatgaggat gagcaccatg gtggcggtga
    35401 gttgtgccca caaaatttgc cagggggtgc gcccgccgcg ttcgatcagt tcgttcggtc
    35461 cgtattgttt tcggcgtgcc gatgattctg attcggtcag gccgttctgt tggtctgagt
    35521 ttaaatttga gagactttcc tgaatgccaa gcaagtgcca attcatgatt cgttatccat
    35581 tctctttgtg ttcatccatg ccagtgcggg gatgcgggcg cggtattgat agacgacgac
    35641 tgcggccatg gccagcagtc ccagcgtgag taccagcggc aggtgctgtt taatggcttc
    35701 actgaccagc atccattcgt ggccaaggac ataacccgcg ccgatgagaa gtgtacacca
    35761 aatataggca ccgataaaag ttgctgcgaa gaatttcaga aatgggattt tcgccaaccc
    35821 ggccgggatg ctgatgagcg tgcgaattcc ggggatgatt ctgccgatca ataccaatcc
    35881 ggaaccccat ttttgcattt gttcttcggc tttgttgatg agggcggggt caatgcggaa
    35941 gaacctggcg aaacggtcaa tgacgggccg tccgcccagc cgcgcgatcc aataggtgat
    36001 ggatgcgccg agggtgctgc ccaacccggc atacacgcct gcgatcagca caaaggtggg
    36061 tgcaatgccg tgccgctcga tgagcatcca ccccgcgaag gcgaggatga tctcgctcgg
    36121 cgtaataccc gtggcattct cgaataccat taatccgaat acacccgccc agccaaactg
    36181 gtcgaagatg gtttgtaatg cggaaaggat cgttgtttcg atttggtcaa acatggtacc
    36241 tctgttgttt tagatctggc aggtcttttg aaaataaaaa gaccatcgcc aaaagatttg
    36301 acgatggtct tgccttttac agaacaaccg ggaaatcatt tccgtattga cggctgtgct
    36361 acccaaactt cgggcggcta ctccccatcg gagtaaatta gagtatacag aagatgtgcg
    36421 ttttgtggat aagaatcctc ttatctaggc ttgcaattgc aatcacagcg gaaccgccat
    36481 gcgtgaagcc cgcaaggcag agcggatcat caaatactcg gcgatattct cagcggtgac
    36541 aatgcccacc aattgcccgc cgtgtgtaac gggcagggcg ggcacgcccg attcctgaat
    36601 gcgcaacagg gcgttctcca ccatttcgta ggaatccact tcggggaggt cgcgtttcat
    36661 cacggctgaa atggcgatgc cttctccgtg ctgggtgagt gccatgagga aatcatcacg
    36721 ggttacaacc ccaacgatgc gctcgccatc gatgactggg aaatcatgct gtgagccagc
    36781 cagcagcaac tgtgacatgc gcgcaagggt atcacgcggc gaaaggcttt tgtattcggt
    36841 cagcatggcg cggccaacag ggattccgct gatggcgttc ttcatttgcg tggcgctcga
    36901 ttcctgcgaa gcgccgatcc agatgaagaa tgcgatgaac aaaagtgtgg cattgccaaa
    36961 caaacccacc aggccaaaca acaacgccat gccctgaccg atggtcgcgg cgatctgtgt
    37021 cgcgcggaca taatccatgc gcatggcgag cagtgcccgc agcacgcgtc caccgtccat
    37081 ggggaaggcg gggatgaggt tgaacagcac caacgagaga ttgaccgtca tcagcctttc
    37141 gaggaaattg cccgaggcta tggtcagctc attgagcggc accaggcttg aggtcaaata
    37201 aagatacgca aacaatccag ccgcgatcac cacattgacg gcgggtccca tcagcgccac
    37261 ccacaattcc tcgatcggct tctcgggcat gcgctccaaa cgcgccacac caccgatggg
    37321 gtacagggtg atatcgcgag tcttgattcc gtacttgcgg gctgtgaggg cgtgcccata
    37381 ttcatgcaaa atcacacaca cgaacagcgc caggatgaac aaaataccgt tgagtacctc
    37441 gccccaattg cgatgttcga gccagtgact gtagccgacc cacccgatca aaagcaggaa
    37501 ggtggcgtga acgtaaacgt caatacctgc aaattttcct aatttccatt gccatttcat
    37561 ggttcgctcc ttgataatgt gagcgcattg tacaaggaat ttattagaaa aatgttataa
    37621 atattctctc aaaatctatc aggatgaaaa aacgcttcgc ccttcctgaa acctgcccct
    37681 atttcaaccc acattccctc cccgaaaaaa aacaggctgg gcgcagtacc ctgcaaccca
    37741 acctgtaact tgaaacctgc aacctgcaac ctgaaacctg aaacctgaaa cccacaacct
    37801 acttcaacaa ccccagcttc ttccccgcct cgccgatgac atccattgcg cgttcgatct
    37861 cgcttggttc atgcgccgca gtgacggtgg cacgcaggcg ggccagacct tcctgcacgg
    37921 caggggaaac gacgggcagc acaaagacat cgttgtgctg acattcgcga gtcatggcga
    37981 aagccatgtc atcttcgccg cacaagacgg gcacaatggc ggtctcggtc agcagggtgt
    38041 cgaagcccat gctcttcaac ccgccgataa actgtttggt gttctggttc aagcgttcga
    38101 tgcgccacgg ctcgtccaaa atgaccttga aagcttcgtt ggcggcggcg gcctgcgcag
    38161 gcgggagcgc cgcagagaaa atgtaggcgc ggctggcgtg gcgcaggtat tgaatgattt
    38221 ccttcctcgc agccacatac ccgccaaccg acggaatggt tttgctgagg gtgcccatct
    38281 tgatgtcaat ttcgccgtac atgttgaaat attcttcgat gcccgtgcct ttttcaccga
    38341 gcacgcccac cgaatgggcc tcgtcgatca tcagccaggc attgtatttc ttgcaaagcg
    38401 tgaccatggt gggcaggtca atgatgtcgc catccatgct gaagaccgaa tccgcgatga
    38461 ccagtttggc tacatcggcg ggggcgtttt tgagcaggcc ttcgaggtgt tccatgtcat
    38521 tgtggcggaa gcggcggaat tccgcgccag acatgagaca accatccaca atggaggcgt
    38581 ggttgatctt gtctgaaaaa acgtagtcac cgcggcccat cagcgtggaa atgacggtca
    38641 ggttggtggc ataacccgaa gtgtaggtga tggctgcttc ggtgtgcttg aaattggaaa
    38701 tggtctcttc caattcggaa tggatggtca gtgtgccggc gagagtccgc acgccgtttg
    38761 tgcccgtacc gaatttttcc acggcctttt tggcagcttc gttgatgcgt gggtggccga
    38821 ccagccccaa ataactgtaa gaagcgtaca tccccatttc acgcccgcgt acgcgcacct
    38881 tgttgcccgg cagaatctct tccacgggtt ggttataaaa gtaaagatca ttgctcttga
    38941 gataatccag gcgatccgtc atggccttga tgcggcgggt gacagcgtcg ttcgtgttgt
    39001 tgaaatccat gtagtgcctc cgaaaaagaa tgtcgcaagt gtagccgagt ggaaaggttc
    39061 tgtctagcaa aatgtgaaga aggctggtgc aataaaggat gaaggatgag ggataaagga
    39121 tgaaaaaagg taacttccac tttccactct ttacccctct gccaatttcc gcagctccgc
    39181 ttcatcaatc actttcacgc ccagcgattt tgccttatcc aattttgagc ctggggcttc
    39241 gccgagcaca aggtagctgg tctttttgct tacgctatcg gtcaccttgc cgccgttgct
    39301 ttcaataaat tccttcacac catcgcgtga aagcgtgggc agggtacccg tcactacgaa
    39361 agtctggcct gtaaacgccc cttctttctt cttttcatct ttcttcattt ccggccacac
    39421 ccctgccgtt ttcagctttt tcaacaactt ctgattggca ttttgcgcaa accaatccac
    39481 aatcgattcg gcgatattgg gccccaaccc atccatttgt tgaagttcct cggcagacgc
    39541 ctttgataat gtggatagat ttccaaacgt gcgcgccaaa tctcccgcca tgacttcacc
    39601 cacgccatga atgcccaacg ccacgatcaa gcgattcaaa ctctgacctt tggcctgctc
    39661 aatagcccca agcagattct ccgccttttt ctctgcaaaa ccttccagtt ccagcaattg
    39721 ttccttcttg atcgagaaca ggtcagccac atctttgacc aacccggctt cgatcaattg
    39781 ctctacgatt ttgatgccca atccaacaat gtccattgcg ccgcgagaga cgaaatgctc
    39841 cacattgcgc acaagctggg ccgggcaggc cgcattgaca caataccaag ccacttcgcc
    39901 ttcgagatgc tccacttcct gtccgcaggc ggggcatttt gagggcggtt tgtatttctt
    39961 ctctttgccg ctgcgtgcat ccacaatggg gccgatgaca tagggaatca cctcgcctgc
    40021 gcgcttcacc agcacccgat ctcctgcgcg gatatccttt tcagcgatgt aatcgaagtt
    40081 gtgcagggtg gctctttcca ccaccacacc gccgatttca acgggttcta gcatggcata
    40141 aggggtcaac accccggtgc ggcccaccgc cacgccgata tcattcaatg tggtggtcac
    40201 ttcacgggcg gggaatttga acgcgaccgc gccgcgcggg tctttgccaa cgaagcccag
    40261 atctgcggcg agggtcaaat cgtcaatttt tatgaccata ccgtcggctt cgtagggaag
    40321 cgcatcgcgt cctgtgctga aggtttctgt gtaggcgatc gccgcttcga ggtcgttgaa
    40381 tcgacgggct acatcggtaa caggaaagcc gagggctttg aggtattcca agatttccca
    40441 ttgggaggtg ggtaccttgc ccccttcaga atggacgatc tggtacacca gcaaggtgat
    40501 cgggcgtgac gcggtgagtt gtggatcaag ctggcgcaat gaacccgccg ctgtattgcg
    40561 cgggttgagg taggtctttt cgcctgcttc ctcgagtttt ttatttaatg cttcaaaatc
    40621 cttgttgggg atgaaagctt cgccgcgaac aacaagatgc tttgggactt gaaagggatg
    40681 aagcgatgac tgcctgccct gttcgcttcc cacagggatt ttcagcggaa ttgcccgcac
    40741 agtgcgcagg ttcgaggtga tgtcttcacc cacttcaccg tcgccgcggg tggcaccctg
    40801 catgaacatt ccatcgcgat aatgcaggac gacggataag ccgtctattt ttggctcaac
    40861 gacgaatttg gctttttcca cgcggtcatc aagctttttg atccgctcga accatgcgcg
    40921 ggcatcgtct gcgccgaagg cgttggccag gctgagaatg ggtgcggggt ggcgaatttt
    40981 ctcgaaacgg tcagcaggcc gggcaccggc gcgttgcgta gggctgtcgg gtgtgatcca
    41041 ctctgggtgg tcggcttcga gttgtttgag ttcattcagc aggcggtcgt attccagatc
    41101 actgatcacc ggtgcatcca acacatggta ccgataattg tggaagttga cctgttcttt
    41161 gagttcttcg taccgggagg aggattttgt tgccatgcct gcattatagc cgaatcaaaa
    41221 cccgcctgaa ttttcaggcg ggtgattggt ttactctatt ttcttccaga tatctgaagg
    41281 cggacgggtg agggggcgcg tcatggcggt gaggcgggat tcgaagtagg tctcttcggc
    41341 tgttgtaaac cttgaaattg cgaacaggtt tcgcgccacg aggatatcgg aaatcttttt
    41401 tgtttcttcg cgggtgaatt ccagtttttc ctgaaattcg gtcatgctga accgcccgag
    41461 gcggatgacg aaattcaaag cggagcgtaa tggttctgaa tatgagacga tgtcggaagg
    41521 acggatatcc tccgtacctg cttcaagttc cttgaccgct agttcaaaag gggtttcgtt
    41581 catggcacat tgggaaagat agcctaggct ttgagctgat ccaccagttt gcgcaggcct
    41641 ttcgtcattg ggtggtcggg gaagcgaatg gcgaaaatac cagagcttgc cagtgtcatc
    41701 atttcatcgg aatgaggcag gacggcggcg actgtggcat cgtaggtctg ctctacacgg
    41761 gtgcggacat cttcgaggtc gaattgaggc ggcaccttgt tgacgagcat cagcatcttg
    41821 ggaacatcga gtttgcgggc cacatccaca gtgacggctg ttccctgata gtcctgtgag
    41881 tcagggcgca ggatgatgac cagtgcattg gagatggcga tcgaaagcag ggtttcttcg
    41941 tttaacccag ggtgggtgtc aatgagcagg taatcgagtt tcaatttttc aacgacatcg
    42001 cggaacccgt cgttaagcag gcctacgtcg taaccttcgc ggaggatgcg ggcgatttca
    42061 cctggtttga tgctggaagg gatgaggaag acctgtcctt tgacttcagc acccaaatgg
    42121 gaagtgacat cgtgagcggt ttcttcaatt gagcattttc cccagaggta atcgttcagg
    42181 gaatggccca tttccgattc atcgagatta aaaaggacat ggatgccggg cgattgaata
    42241 tctgtatcca ccacaccgac acgcgcacca tccacggcca aaagggctga aatatttgca
    42301 gtggtgttag attttcccgt cccaccacgg aatgaatgta ctgagatgat tgtggacatc
    42361 ttccctctcc aagtaaggat ttatgaatta tttctttttt gattatatta cattcgcaaa
    42421 gattaagcag actaaaatca gttctgtagt tttctgttga cggctaaggt tttggggtct
    42481 tctgcaccag ttcgtacagc gccatggggg tgcttttacc tttaagtgag gcttcaccga
    42541 gcggctggca ttcatatttc gtctcaatgg cctgctgtac gctttctgag atgatgatct
    42601 ggtttcgccc ggcgcgattc atcaaacggg cggtggtgtt gacggtatct cccagcacat
    42661 tgtattcgcg ccgcccgcgc gggtcaccga tctcgccgac aaaggcaggc ccggtgtgga
    42721 ttccgatcat gcaatagatc tcaggttgga aattcccgat ggtgggcggc ttgatgttca
    42781 tcacgatatc gcggatggct actgcggcag acgcggcacg catctcgtcg ttggtgtggg
    42841 cggttggaac accgaaataa atgacgatgt ctgagcctga aaggtgatat gtcacctttt
    42901 tcaacatgcc gccacgagat tccactacgg cgttgattaa ggaaaaagcc ttcgaaaagc
    42961 tggtcgagag cttgagttct tcaccgggca gggcgcggtc ggcggcctca ggcaacccca
    43021 cgaagttgat gaacatgatc gtcggtttgg ggaaatcagg gtcgatcttc ctttgagcgg
    43081 cgctttccac cagcaaagaa aggacgggaa aaggcagaaa actggccatg ggttcaatgg
    43141 aattgaccag atcggtgatt tcttttaaga ccgattgttt gtcgcggtct atcagggtgc
    43201 tggaagccat gcggcggctg accggggtga tctcatattc gccgagttca ttatcggcca
    43261 ggtcatcttc caccagcatg tagtactggt ctttttcatt ttcgatcttt tcgccctgct
    43321 caaagcgaaa atgttcaagg actctttcgt atgccgaagg actcaggttt acgcgttcat
    43381 tacgtccctt gctttcggcc agtttggctc tttgtacatc ccgcccaagc aatacatgct
    43441 ccatgcggcg aggggtgcca atatcagagg tgagaaagcg gcccgagtgg atgccgatgc
    43501 gcattttcaa agtgacaatg cctgccgagg tctcaatatg ggcaaactct ttcatggccc
    43561 gttgcatccg cagcccggca cggacggcgt gcagggtgtc atcgtttcgg tctcgtttgg
    43621 gaaacatgat cagcagggca tcaccggtaa attcgaccaa gtcaccgccc gatttgctga
    43681 tgatggcgat cattgtggag aaatattttg tcaggtgttt caggagttcg gcagcgcctt
    43741 cgcgtccctt gtttgaactg gcttccatca atttggtaaa gcctgccaga tcggtgaaca
    43801 tcatggtgcc atactgccat tcggttttga tgttgccggg tttcatcgtt tgagatgaga
    43861 tctgccgtga tgagtagtcg tgcagaatgc gttggagcgt ccgcaggtgc ttgtgtacgt
    43921 cgatcatcat gtcaaccgag ggattggtcc acatggaggc atacatctcc gccgggagca
    43981 ggtatcttaa tcggctttca atggcggcaa gcggcgattc tggcagcatg ttgaaaatta
    44041 taacttgatt tcatgtctca aattatgtag tgacattgaa atagccccgc tccatttgta
    44101 ctggttataa aaataaaacc agatctattt tcggctgcgc aactgggtgg ctttatcttt
    44161 taattcctga aaatagcgtg tctctgtaat ttcagccacc tcgcgcgagc ggcgcgattc
    44221 gttgatctcg atctttaatt tggcgatctc ctgccgcaga gacgcttcac ggatataacc
    44281 gtccaatgcg gcggttgcaa gcaggatcaa ggcttcaagg atgtcgtcat ctttgaagga
    44341 gatgatctca ccggtggcgg gatccttggc gttgatcaat tgcagaacac cgatcacctt
    44401 gtcttctgaa tttgtgagtg gaattgcgag gaaggatttg gagcggtatt ttgtgcgggt
    44461 atcgaaggct ttcgtcccgg agaagtcgaa gccatcggct tcataggcat cagcgacatt
    44521 aatgcggttg tgggtaagtg cagcatgcga caccacgttc gcccgattct cactgccatc
    44581 ggctttgtat agtttcaccg ggtaaaaagg aatctcattg ccgctggttc cacccatggc
    44641 catcccaagc gaggtgtttc tcaggatcac aaatttgagc gcgttgtctt cgagaatgta
    44701 caacgtgccc gcatcggcgt tggtgaagaa ttgagcctcc acaaccaggg tttcaagcag
    44761 gcggttgtag tctttttcag ccgatagggc caccccgatc gggatcactt tgcggagcaa
    44821 acgcagctgg tggtctcgca ccgcaatgcc atgtgccatt ttatcgagca tgcgggccag
    44881 ttgaccaagt tcaccgctct cgttaatcag gtcgccaagc aggcctggca catactcacc
    44941 cgcctccact gcatggacag cgttgataat tctctttaat ggggaggatg ggctgattaa
    45001 ggtggggttt gacatgtttt tcctgcggtt gcaaaagtat accaccagct ttctctgtaa
    45061 catggaccag ccccgtctaa aagacggggc tggttgatta tcttgctatg gggttacaca
    45121 gaggggcttc gcaaatattt gatcaaagcc gccatgagca gaccgatcag ggcaagcaat
    45181 acaagcggga agaaccagag gaaggacatg cctatcccca ttcctccacg tattgggaaa
    45241 gagccaattt gattcatcat acccatattg ccgtggctca tcatgccaaa gccagcggac
    45301 ggaaaaaaga cctgccagag cgaagggagg aagaacaaca caatcagccc aagtgtgatg
    45361 ccgatgccaa gaatccattt gttttttgta gacatagtga tctcctttat tgtctttatc
    45421 atagcggcct aatataaagg gactgtaaag aaaacaaaaa catccattaa aactttgaga
    45481 tatactttca acatgccacc tacaaacatc ggaatctttg gcggtacctt tgatccgccg
    45541 catatcggtc accttgttct tgccagtgag gctgttcagc agcttaacct ttcccgcctg
    45601 ttatgggtgc ttgcgcccga gcctccgcat aaattggacc aggccattac gccgctttca
    45661 caccgccttg aaatgttgaa gcggaccatt gcagataatc cgtcttttga aatctcacgt
    45721 gttgaaattg accgccctgg cccccattac agtgtggata ctgtgaaact gtttgccgaa
    45781 ctataccccg atgcggaact cacccttttg ctcggcggtg attccctgcg tgacctgccg
    45841 acctggcgcc gctatactga actggtgtct gctgtttcca aaattggtgt gatgcgccgc
    45901 ccgggcgtct cttttgacat gcctgctttg gaaactcaaa ttcccgggat aacgcaaaag
    45961 gtctggttca ttgatgcatt gctgcaaaat gtatcttcgc gtgaacttcg ccgccgtgtt
    46021 gcaagcgggg aaacctatcg gtattatgta cttccctctg tgtatgatta tattgaagcg
    46081 aaccatcttt atggaagata gttctttctc tgttgatctt caaaaacact caattcttta
    46141 gccgcaggtt tcgcgaattt acacgaaaaa agccgatcca ttttacaggg tcggcttttt
    46201 tataactcgt ccgtatcttg caattcattt gactgcttgc gtaaggttta gggaatatct
    46261 ttgaaaagcc attttgaagc acagagacac tgagtcagtg cggagcctcc tgcaggcgga
    46321 gtttttattt ttttctctgt gtctccgtgg ttaatctttc agacttttgc ataaattcgc
    46381 gacgaaccgc gtatttcgca actgactcct tacgtttgga gtaaatcatg ttttacggtt
    46441 caaataaaaa gttaagcccg tgtgagtttt gcccgcatca cgtattggtt cacggcgccg
    46501 aagcgg
//
LOCUS       DAOQJC010000013        44941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_344_length_44941_cov_11.040748, whole genome shotgun sequence.
ACCESSION   DAOQJC010000013 DAOQJC010000000
VERSION     DAOQJC010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 44941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44941
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_344_length_44941_cov_11.040748"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..908)
                     /locus_tag="PLE39_03230"
     CDS             complement(198..908)
                     /locus_tag="PLE39_03230"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNE67364.1"
                     /translation="MSEKTFRKLAGIFFIVGAILVNIPYTLLIINFDYPDILRLPAAE
                     ILTRFQAGGSSLIYTWLAFAWVGLPMLLGAIMLKRILADENAPFLETATTLGVIGFIV
                     QVVGLLRWVFVVPVLARLFTDPTTDPVTKAALPLVFSAVHQYGGVILGEHLGQFLIIL
                     WMSMLSGILYKSKRFSKWVAYLGWIASAVYILAQTELLATAIPSFPVIGWAGLYGSLL
                     WLVWMIVVGVYLVKNSEQ"
     gene            complement(949..1530)
                     /locus_tag="PLE39_03235"
     CDS             complement(949..1530)
                     /locus_tag="PLE39_03235"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE67365.1"
                     /translation="MSTREKILDTALTLFNDQGTAAVSTNHIAEAAGISPGNLYYHFR
                     NKDEIIHELFERLFAANDAAFSLSADTLPTLADLQNMVKANYKILWQFRFAHRELAAL
                     LRADGELRSRFLSVRKRGFDGFTQLFAAFTSAGVLRAANPTVIENLAETCWLITEFWL
                     NSLEIGGKAVTEAQMERGVQLMMQSLEPYIAQP"
     gene            complement(1632..>2958)
                     /locus_tag="PLE39_03240"
     CDS             complement(1632..>2958)
                     /locus_tag="PLE39_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNE67366.1"
                     /translation="MPQATYHFPKGFLWGTATASHQVEGNNTNNNWHAWEEAGHTVHK
                     SGLAADWWGGRWREDFDRAAETGQNAHRLSLEWSRIQPTPDTWDEEAIEKYRTMLRGL
                     RERNMTALVSLHHFTDPLWVTERGAWENAEIVPLFEKYVRKVVGALKEYVTLWCTINE
                     PNVYALGGYVQGAFPPGKKDIRLSMRVLGNMLRGHAAAYRAIHELQPESRVGYALHYR
                     PMVPRLSWSPLDRLMRNIRYNGINMGFPSAISTGVMRSPVGNQNIPEAKGTQDYLGLN
                     YYSVDTVWFDLFKPGELFSNSGYPKEADMSDTNFIANIPEGIFNSIKWIERTYPNLPI
                     IITENGVEDSDDHMRPRYLAQHLHQVWRAVNFNWQVKGYFHWSLVDNFEWERGWTQRF
                     GLWGLDLDTQKRIRRPSVDLYAEICKENGISSEMVQKYCPEVFKKIFPS"
     assembly_gap    2959..3058
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3059..3741)
                     /gene="rlmB"
                     /locus_tag="PLE39_03245"
     CDS             complement(<3059..3741)
                     /gene="rlmB"
                     /locus_tag="PLE39_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNE67367.1"
                     /translation="MKELIYSRNAVYEVLRAKRRQVFSIEIAEGVQEKGRLTEIVKMA
                     QQQKIRISRVPRPKLDKVHQNNQGVVAEVSGYPYSDVVEILEQAKEELPFVLILDSLQ
                     DPQNFGTLIRTAEAIGVHGVVIPLARSVDVTPAVVNASSGASEHMLIAQANLSQTIDA
                     LKDNDVWIVGLDQAGAEVEAGSRHLKGALGLVVGSEGEGLHDLVRKKCDIVLKLPMKG
                     RIESLNAAVA"
     gene            3796..4263
                     /locus_tag="PLE39_03250"
     CDS             3796..4263
                     /locus_tag="PLE39_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE67368.1"
                     /translation="MKKEFTIATISTKDSPFINDLCKLRDGLSAHHDDTTSSERFRQF
                     MLKHLGEETMLTFLILNNGRPIGYGLAFDVIEHAYMPEWTRKGYITQFFVNAEYRGQG
                     IGEMGLAYIHDWFRARGITDVLLNVAIKNEVGNRFWQKNGYTPYATRMICRLK"
     gene            complement(4264..5709)
                     /locus_tag="PLE39_03255"
     CDS             complement(4264..5709)
                     /locus_tag="PLE39_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNE67369.1"
                     /translation="MPTLMLPKSGKPKADILESMRRARDKDAQWQNGKVFGLVYHISE
                     EIDDLLKEAYTMFFSENGLNPTAFPSLSKFENEIIAMASNLLGGDDKVAGTMTSGGTE
                     SLLMAVKTARDYARAKRGITKPEIIMPSTAHPALDKAGEYFNVRMIHIPVREDLRADV
                     DATRRAITPNTIMLVGSAPSYPHGVVDPIRELAALAQEHGLLFHTDACVGGFMLPFVR
                     KLGYPVPEFGFDVPGVTSISADLHKYAYAAKPASLVLYRTSELRRHQMFVSTDWPGGI
                     YPSPSMAGSRPGGPVAAAWALLNYLGEDGYLEITDIVMRTAKILQDGVNEIPGLKVLS
                     NPEMSVFAIASDSTDVYELADELALRGWHLDRQIFPPSLHVTVNYVHAQVGAEFLTDL
                     ANAAERVRRPGLRKTATKFLVQAANLLTRVLPEETVSNLMDKASSLLGGGGGLPARMA
                     PMYGLIGSLPNRGDLKELVLDLLDNINSYKE"
     gene            complement(5779..7176)
                     /gene="cysS"
                     /locus_tag="PLE39_03260"
     CDS             complement(5779..7176)
                     /gene="cysS"
                     /locus_tag="PLE39_03260"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNE67370.1"
                     /translation="MLKIYNTLTRKTEEFQTLEPNLVKMYVCGVTVYNDAHVGHAMSA
                     LVFDIIRRYLEYRGYAVKHVMNYTDVDDKIINRANQLGEDPLKLSQRYIEDYANDLKS
                     LNVLPATSNPQVSKTMPLIIQFIEGLIQKGHAYAATNGDVYFRVTSDDDYGRLSGRKL
                     EDMQAGARIEIGEAKDHPMDFALWKAAKPGEISWDSPWGKGRPGWHIECSAMNLAELG
                     EQIDIHGGGNDLIFPHHENEIAQSESYTGKEFSRYWIHNGMLQLGGEKMSKSLGNIVS
                     IKEFLSRRSADVMRMLVLNGTYRAPLMFNEDTLDAAEKNVERLKSALRPASASSQGLS
                     AEAASALTSASESAKANFTEAMDNDFNTAGAVAALFELSKAINTARDNGATGDQLKAA
                     QTMFGELAGVLGLKLEEKKGSSEVEAQVIALIAERTEARKQKQWKRSDELRDQLKEMG
                     VTIEDSKDGTTWRWG"
     gene            complement(7289..8374)
                     /locus_tag="PLE39_03265"
     CDS             complement(7289..8374)
                     /locus_tag="PLE39_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNE67371.1"
                     /translation="MSFEFILRILAMFALAIGGGMWGFNFTKANPETDPIVTTTLSTV
                     VGALAGIILTPYFTTRPARYLRSLLGRLAAESLFAALTGLIVGLLIAALLGFPLSLLP
                     EPFGQILPFLGVLVFSYLGISLFTMRQGDIMGLLSALSGRGEGGGSSSWTNLNRNILL
                     DTSVIIDGRVADIAKTGFLPGTLLIPRFVLNELQYIADSPDGMRRQRGRRGMEVLSEL
                     QKLPNVLVRISDINVEGVREVDDKLIVLGKQLKSPVLTNDYNLNRIAELQGVTVLNIN
                     ELANAVKSVVLPGETLRINIMQEGKEYGQGVGYMDDGTMVVVENGKDYIGEYMEVNIT
                     KVLQTAAGRMIFGRVDEENSGKKGKGK"
     gene            8488..9468
                     /gene="moaA"
                     /locus_tag="PLE39_03270"
     CDS             8488..9468
                     /gene="moaA"
                     /locus_tag="PLE39_03270"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNE67372.1"
                     /translation="MAIDRFGRNIHYLRISLTDHCNLRCVYCMPEDMTFRPNADLMQD
                     DEILTFARLFASLGFDKIRLTGGEPTVRANIVDIVRGISSTEGIRSVSMTTNGLLLKK
                     LAQPLADAGLQRVNISIDTLNPNKFKRLTRWGKVEEVWEGILAAERAGLTPVKLNAVV
                     VKGYNEEDVIDLAALTIDHPWQMRFIEMMPFGGATDLQINQVITMKQIQDNIESEFGK
                     LESANDGKLDGEAQVFHIRGAKGNVGFISSVTHPFCSACTRARLTSDGKLRLCLLREK
                     EVDLLTPMRAGASLEELRRLLLDGVWEKPWGHGLADGVIPLNRVMSEIGG"
     gene            9491..10183
                     /locus_tag="PLE39_03275"
     CDS             9491..10183
                     /locus_tag="PLE39_03275"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNE67373.1"
                     /translation="MFDLDGTLRHHLPSGGEVFVEFVRSMGFKVSEEDRIRAEHWEHF
                     YFASSLEIRKDSETYKDDRSGFWVNFGRRRLIALGLKASDALEVAPKASAHMNEAYKP
                     QVHVPPDAHTQLTALKEAGYILGVVSNRETPYQDELKELKLDSYFKFSLAGGEVNSYK
                     PDKIIFERALELAGTSAAETMYIGDNYFADVMGAHRSGLKPVLYDPTNLFPDTGGFAF
                     IRSFAELPELLK"
     gene            10281..11630
                     /locus_tag="PLE39_03280"
     CDS             10281..11630
                     /locus_tag="PLE39_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNE67374.1"
                     /translation="MAWERKLIRTVRVRNSQKTGVLARLLTAIAEAGGNTGSLVLLTE
                     TSQNVVRDITITAESEAHFETILDAMRSNEGTRIIEIRDEVLELHQKGKIAIRSRYPI
                     ESLTTLRRVYTPGVAEVCRKIAKDPSQARLYTSISHMVAIVTDGTAVLGLGDIGPLAG
                     MPVMEGKAMLMETLVGLSGIPILLNTKDTEEIISTVAAISPTFAAIQLEDISAPRCFE
                     IEERLQAMLDIPVLHDDQHGTAVVCTAALLVATRETGTDLNKAVIGQIGLGAAGHAIG
                     VMMMRLTGNPVYGADLSPEALERFEKAGGKKSNLREVMEKCDIVIATTGAPNLIKPEM
                     VRKGQIILALSNPNPEIDPEVAMEHGAAFAADGKSVNNVLGFPGIFRGAVDANAPRIT
                     HEMLLAAAHVIAEMTPPGELVPSPLDKKVHRAVARAVAETAIKQGIARAEYVPYVEE"
     gene            complement(11703..12932)
                     /locus_tag="PLE39_03285"
     CDS             complement(11703..12932)
                     /locus_tag="PLE39_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNE67375.1"
                     /translation="MQTPWEYRYAHRTQKMGSSVIRELLKFTEQPDIISFAGGLPAPE
                     VFPLKEFQEACNQVLTDHGPQALQYSTTEGYLPLREMIARHNARYSVRVTSDNIMITS
                     GSQQALDFIGRLFINRGDYVVVESPTYLGALQAWNAYGAQYISVPSDEDGMIVDQLEE
                     ALRIGPKFIYVLPNFQNPSGSTMSLERRKRLVLLADQYGVPIVEDDPYGQLRYDGEHI
                     PSVATLDSRYRNEDNGEYTGNVIYLSTFSKLLAPGLRLAWVIAPPQVIRRLVMTKQAA
                     DLHTSSFNQHVAYEVAKGGFLDEHVKVIRATYKERRDVMLELMDELFPSDVRWTKPQG
                     GMFLWGMMPENVDAAEVLKVAIDRKVAFVPGAAFHPNGGGKNTMRLNFSFSSPENIRE
                     GISRLALTLKEALKNGK"
     gene            complement(13107..13526)
                     /locus_tag="PLE39_03290"
     CDS             complement(13107..13526)
                     /locus_tag="PLE39_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE67376.1"
                     /translation="MADQIFPEPTVGVFIFNKKGELLLLQSHKFPGKYVVPGGHVELG
                     ERLEEAAIREAKEETGLDIYDLKFINFQQFIYDPSFWKRRHFIFFDYACRTDSESVQL
                     NDEAEDHVWVSPSEALHLPLDSYTRTSVEHIIAGNLS"
     gene            complement(13558..14412)
                     /locus_tag="PLE39_03295"
     CDS             complement(13558..14412)
                     /locus_tag="PLE39_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNE67377.1"
                     /translation="MPILALVLLFFSAAMHATWNFLLKSAEEKYIAMGWQVILSGVFS
                     TVLIFFTGLPPRSMWTFAVISMILEAIYFILLCIAYSDHDFSLVYPIARGAAPALLVL
                     WTALFLHEQLTTGGYIGLAMITGGMILIGATTLLQNHEEKPHLRGILTALSVALIISI
                     YTYIDGNAVKHGPALPYGLSMFVMVPFVTTPYLVQRYGWSHFSAMWRQKRGYLLMGGL
                     LGLVAYMLALFAYTFAPLSYSGAIREVSAVIGAFLGWRFLKEEMGGIRVVGSAIVFAG
                     VMVIAVFG"
     gene            complement(14416..15279)
                     /locus_tag="PLE39_03300"
     CDS             complement(14416..15279)
                     /locus_tag="PLE39_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE67378.1"
                     /translation="MQSQFVKTNSINLHVMTDGPENGTPVILLHGFPEFSYGWRKQIP
                     ALTAAGFRVIVPDQRGYNLSDKPKGVSSYAVDVLARDVLGLFDHFGIEKAFLVGHDWG
                     AVVAWTVALNHPERLKKLAILNVPHPDVMTEFVLKNAEQRKKSWYVFFFQIPLFVEWM
                     LSKNDFHNLGRMLVGSGRKSTFTLQDLAEYKKAWAQPGALTGMLNWYRAVFRRSLWHV
                     FGKKISARRITIPTLMLWGKRDVALSHEMVAPSIALCDDGKTVLFEKSTHWVQHDEAD
                     EVNKNLIEFLR"
     gene            complement(15284..15955)
                     /locus_tag="PLE39_03305"
     CDS             complement(15284..15955)
                     /locus_tag="PLE39_03305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE67379.1"
                     /translation="MKTIVITGATGVLGNLVAKTFAADGHNIALLDRDETKLESLVRE
                     LNLPSERLHAQTIDLLNGQAVQDSAEAVHSKFGSVHALIHLVGGWTGGKTIPESNAAD
                     LDAMINQHVWTTYHLLKSFAPHIANSREAWGRVIAVSMPVTVNPSAKMALYAAAKSAQ
                     ESLVLTLAQEFKENGLTANVIHVKSIDVKNEGKGTSPAEIVSAMQYLFSEEANKVTGA
                     RIPLY"
     gene            complement(16010..16768)
                     /gene="ribA"
                     /locus_tag="PLE39_03310"
     CDS             complement(16010..16768)
                     /gene="ribA"
                     /locus_tag="PLE39_03310"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HNE67380.1"
                     /translation="MTTFTHEKIQLLMEEDCCHECEGYGSDKICVHIEAIAELPSRFG
                     DFHIVAFSNNRDGKEHVAIIKGEVLGAEDVPVRLHSECLTGDVVGSLRCDCRDQLEAA
                     LQKIGEMDKGIVLYLRQEGRGIGLTNKIRAYSLQDQGLDTVEANLALGFRDDERDYAV
                     AAHMLHSLKIQSIRLMTNNPKKIAQLEQHGVKVNERLPHILPSNEHNRFYLETKAAKS
                     GHMIDFHGKPHLVEQSDPPIVAGMTEDQIQALYE"
     gene            complement(16802..17488)
                     /locus_tag="PLE39_03315"
     CDS             complement(16802..17488)
                     /locus_tag="PLE39_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNE67381.1"
                     /translation="MNRPFTFINVAMTADGKIDTFERKGSAISSKRDKTRVDELRAAA
                     DGILVGGRTLLDEAPKLTVKSEALREGRVQRGLSPNPIKVGVVTRITNADIPPDSDFI
                     KAGPSRVVIFTTNQTSKDKLAELTACGVEVFVHDAPRVDLNSMMLTLKKIGVDHLMVE
                     GGGTMNFELMRLGLVDELMIFVAPMIFGGANAPTPADGVGLPRDVAMGLKLIDVEKWE
                     DGGVLLKYRL"
     gene            complement(17519..18289)
                     /gene="ygiD"
                     /locus_tag="PLE39_03320"
     CDS             complement(17519..18289)
                     /gene="ygiD"
                     /locus_tag="PLE39_03320"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HNE67382.1"
                     /translation="MTEKMPVLFVGHGSPMNAIEDNEFSRAWRDAGKALPKPRAILCV
                     SAHWVTRGTLVTAMEQPRMIYDMYGFPKELYEVKYPAPGSPDLAEQVRRIIKKTEVYA
                     DQEWGLDHGTWSVLNQMFPEANVPVIQMSLNANLSINDHYEVAKQLKELREEGVLIIS
                     SGNIVHNLSMVQFRDFAYDWATEFDTQVKNWILANDHDPIINFQKQGQAAQLAINSGE
                     HYVPLLYSLALKDDDDKVSFFAEKVWGGSVSMRSVRIG"
     gene            complement(18299..18721)
                     /locus_tag="PLE39_03325"
     CDS             complement(18299..18721)
                     /locus_tag="PLE39_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNE67383.1"
                     /translation="MNTNQRFAISIHALTLLASSNDPLTSEMIASSVDTNPVVIRRTM
                     ASLREHGLVESKSGAKGGWRLLREPKEIVLCEVYRSLDEVNVLAVHSHPNRHCPIGGN
                     IGGVLQSVFADAQSEMEKALGNYTVADILKDVRRKANH"
     gene            complement(18825..20426)
                     /locus_tag="PLE39_03330"
     CDS             complement(18825..20426)
                     /locus_tag="PLE39_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNE67384.1"
                     /translation="MNPIRSRLTRIVSVVLLFSFLASCATPTPAPTPQVFAPFILATR
                     APTLPTTTPFQPVAQTDTSVPLLPTSTPTLQPTVLPSATFTQFPTQTQVPPTNPPPAV
                     TFTSPPPSAPSRTNYILYATMDFASRTLDVEETIRYYNGTGLTLSDIVLSVQPNLYGG
                     AFVLNTISQDSVALTTYTLNGQALTMNLPQPLPAGSATTLTLSFRLNVPAKGAGLFGY
                     DFNQINLVDWYPFVVPYNGGWVLHDPMPFGEHLVYDAADIELNIKTEAGVVIAAGAPS
                     EPNGEWTRYRLYGARTFSISASDEFLVTESAVGSVVIRSYYFDGYGAAADGILYGAVQ
                     AVSLFDVKFAPYPYQSLAVVQTDIHDGQEYDGLVFLSTDFYSQYGGGAKNNLTTIGVH
                     EIAHQWWFGLVGNDQALEPWVDEAMAVYSERIFYEYNYPRYGDWWWQFRVDYFGAGGY
                     VDASIYNFGDQRSYVNAVYLNGAHFFEDLRIRMGDDNFFRFLKDYAAIHSRGRASSFD
                     LFAAIRANTSADISDLIAAYFSQSY"
     gene            complement(20426..21916)
                     /locus_tag="PLE39_03335"
     CDS             complement(20426..21916)
                     /locus_tag="PLE39_03335"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNE67385.1"
                     /translation="MRKNIQSSISTLFVFIFLLSSCSTPSPSPTALPTSTAIPFSTST
                     PLPTASNTPLPTATTVPAPERARYVLDTLIDYDAHTVTVDETIYYPNHTGNQLNALVI
                     AIVPNLWEGSFNLNGLSINGTPITTYNINGQRLDISLPFFFKPEEVMVINIQFTLNLP
                     FAEQEDPSVSRPRIYGYTRRQLNLTNWYPFVVPYINGDWVLHEPWYYGEHLVYDAADY
                     QVDVRFADPANVPIVAASGAPEQQADSTRYTITAARTFALAASPEFQVSSLQVGDVIV
                     ASYYFPLSKIAGRAALQASAEALQIYSQKYGPYPHKTLSMVMGDFNDGMEYSAFFYLS
                     RDFYSLYDETPANYLIFVAVHETSHQWWFDQVANDQAQQPWLDESLATYSELVYYETL
                     HPDLVSWWWAYRIDFYNPQGFVDIPIYDGQGFRPYTNAAYFQGAHFLSELRARIGDDA
                     FFAFIQDYLLQGRNKIMTTSDFFRILGEHTLTDTSDIVRQYFQNIY"
     gene            complement(22013..22456)
                     /locus_tag="PLE39_03340"
     CDS             complement(22013..22456)
                     /locus_tag="PLE39_03340"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE67386.1"
                     /translation="MTIIEASIKINKSVEDVFEHLTNIDNMKKMSEYITAIESDGPLK
                     LGAKYKITTTSFGKSTTITNEIIAFEKNKLISTKTYGTPPAPDLVNTSILEADGSGTK
                     LTGQMDAVLLPPGMPNMPGMEDMMKKQLITGIENTLASYKKAIEG"
     gene            complement(22503..23318)
                     /locus_tag="PLE39_03345"
     CDS             complement(22503..23318)
                     /locus_tag="PLE39_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNE67387.1"
                     /translation="MNWLLEPLAYEFMQHGLLASVIVGVLCAVMGTYVVLRGMAFLGD
                     AMAHAILPGVAIAYIFKGDLLIGAGIAAVAVALIIGLFTKDGAVKEDTAIGILFAAAL
                     SLGVALISTTQTYTVDLSHILFGNVLGVSVSDLWLIGGLSFAILLTVALLFKPFLVIS
                     FDPVLAATLRLPVELLRNLMLVLLALTVVVSLQTAGVSLAAAMLVTPAATAYLLTRRL
                     LPMMFVSALIGALSSVVGLYLSYYFNIVSGSAIVLTATAIFLLGFLWKRIKSV"
     gene            complement(23315..24121)
                     /locus_tag="PLE39_03350"
     CDS             complement(23315..24121)
                     /locus_tag="PLE39_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNE67388.1"
                     /translation="MTHTPHRLEVQNISIGYGDKIIMHDLSFQVPHGSRVAVVGPNGA
                     GKSTLFKALVGLLPLKSGQIFIHGEALGVHKDCVAYVPQREEVDWRFPVTVNDVVMMG
                     RFGQTGWLNRASAYDRGMVHKSLEQMGIADLAEHSIGQLSGGQQQRAFLARAIAQEPH
                     ILLMDEPFTGVDATTQETTLRLLDHLQEREVTAIVSTHDLNLAASRFDLVLLLNHRLI
                     AFGKPQEVFVKENLAQAFGNSLLVMEGGMMLVDECCPPDEEHHHHHVEAE"
     gene            complement(24190..25128)
                     /locus_tag="PLE39_03355"
     CDS             complement(24190..25128)
                     /locus_tag="PLE39_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNE67389.1"
                     /translation="MKKIFNTIIVMSTLTALFLTSCGSASTGSDGTLSVLASTSFLAD
                     IAQNVAGDRTQVQSLLPIGADPHAYQPAPADVAKIAKSTVLIVNGVEYEHFIESLLEN
                     AGGERLVIEATAGLTPLQMEEHADEAESGDSHGNEAGDPHMWLDPNLVITYVENIRDG
                     LSKADPAGAETYASNAEAYITQLKELDAFITEQVETIPADRRLLVTNHEAMGYFATRY
                     GFSIVDSILPSFSSEASASAQEIAAAVDAVKNSGAPAIFLGEVENADLANQIASETGV
                     KVVDGLYLESLTDGGPASTYIEMMKNNVIQIVNALK"
     gene            complement(25181..25597)
                     /locus_tag="PLE39_03360"
     CDS             complement(25181..25597)
                     /locus_tag="PLE39_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNE67390.1"
                     /translation="MSSENWLQQLHDNGYRVTSARRAVVDTVYGNSHALTPIEVYDQA
                     RKKYRALGLVTVYRTLEKLEELHLIQRVHQPIGCQAFISAGVGHQHLLLCQNCGQVEF
                     FEGDDLDTLTKSIARKTGYQIQEHWLQLFGLCANCK"
     gene            complement(25718..28234)
                     /locus_tag="PLE39_03365"
     CDS             complement(25718..28234)
                     /locus_tag="PLE39_03365"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNE67391.1"
                     /translation="MKKTKLTLSLLLLLLVFTQAVPVLAHPADVYTHHIYVNIAQGGV
                     FIKWEIRPGPLLIQSIWFNADADQNGTVSDEEGLAWANDHLSKITSLLNGVKMPLTLD
                     GVKFPSSVDLFQAGQEFIVINLSASPIDSDAAYQLIFRNGFEEQKSLNWYYITTTDGM
                     KFETPQQKNAQIALKIFPPSTQDSAQVKLLDAWDSSMPSLSGWQGGTPPATPEPAAPT
                     PEQNANLERLLNLVRAKEFSLSFYIFALGISLILGALHALTPGHGKTVVAAYLVGSRG
                     TVWHAVVLGSVVTLTHTGSVFLLGIITLAASKYIVPTDIIPWLEILSGLLIVGLGLYL
                     LWQRFVYWWKSRQPAPVEEKAARKISLTPSTAPKKVTGVTGIQKPSANMHHHGDGKLH
                     SHDVPEAITWRSLIALGVSGGLVPCPDAIAILLVAVAINRIFLGLALIVSFSLGLAVV
                     LIAIGLLMVSSRRLFDKVSFLDKVAPIMPIVSAVVVLALGFALTWGAYVRAKDSLGLG
                     EAGTGGVEDARIVYQLEGQDRIKQLFISDIRGSQPEALTSGPNSAVDFAPSPDGSRIA
                     YIAQTGDLKNEIWLVDVNSGENKKLTDCTEAMCSQPVWSPDGSRVLYEHISLEAANAT
                     GLATLWWVDVNTGTARSLFQEEQLPGANPRWSPDGKWLSYATSENIQLYNLESGETRT
                     VASALGASAAWAPDGKSVLYRDVIIQSNQFITQLFIYDLETDAAVNISPDLGFENILA
                     AWSPDGEWIAVVRRDLSIPRGDQIWVMRRDGSEARAVTDSPNSLHGTLSWSGDGQSIL
                     YDLYDLDAFPLASKLQMVEAATGKVTDLGIAGYNPKWLWP"
     gene            complement(28242..29603)
                     /locus_tag="PLE39_03370"
     CDS             complement(28242..29603)
                     /locus_tag="PLE39_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67392.1"
                     /translation="MKPRITLDTVALGLIALLVVAAGLVILFSGQIGVRVTATLPERS
                     LIGPFQKIKLTFSESVDHELAESLFSIQPKVDGTIEWLDARTMQFVPTRPFERGASYK
                     LTLSPGRLTQNGSEIKSSQSWDMRVRDPLIVYLVANNDQSILWTVELDGSSPQPISPE
                     EIKVIAFDASADGESIVFSSANSQGGIDLWRMSRAGGDAAIMLDCGRDRCTTPAIAPN
                     GVLIAYTREAAGVGPGLPYGSPRIWVLDLQNGETGPVYEDQQVLGYKPSWSPDSSKLI
                     SFDGLADQFRLFNFTNNEQILFQSNTGGAVTWSPDGTKFLYTDIDLSENGLVTRLRLA
                     DLALNDTSTLLGNSDSRDYAYNSMAWSPSEDSVVVSLRNNEGEPAQVFWLFDPGLLDG
                     IIIADQPGYTYNSPLWDPWGTALVFQQFKLRGEFTPEIGLWQHGFREPLTIAEGIMPH
                     WLP"
     gene            complement(29600..30343)
                     /locus_tag="PLE39_03375"
     CDS             complement(29600..30343)
                     /locus_tag="PLE39_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HNE67393.1"
                     /translation="MPKTLYRSFQALLLLGLCIFFASKAASGQLTYYINLRFVPLTIF
                     GIILLAILAQTLFTEIKRSRQRKGGDHDHDHDHEPVSANLWFMLLPLLIGVLIPARPL
                     DSSALATKGFNTNAPLVSADSSAQLFETDSEERTILDWIKLFNYQSDLEQFIGQKASV
                     IGFVYFTEELGENQFFVSRFVVSCCAADGFAIAMVGEWPQASTLEKDAWVLVKGPIKI
                     ITLNDRPVPLIQAESVQSVPVPEQPYLFP"
     gene            complement(30353..31366)
                     /locus_tag="PLE39_03380"
     CDS             complement(30353..31366)
                     /locus_tag="PLE39_03380"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNE67394.1"
                     /translation="MQNKTSPRLLSRTSIVTIALVVLVLVVASMLPAGFFAVVWERLN
                     VFATVFLGIFVEAIPYLLLGTLASGLVEVFLDRDQMSRWISRRPVAAAVGGALMGMVF
                     PVCECGVVPLTRRLFNKGLPLSAGIAFLLAAPVLNPIVVLSTASAFGWGQMLLWRMGV
                     SLLIAVVVGLVFSVEQDASAVLRPVLTSAHEHDHSMDASASFNEKMRRALLVTADEFF
                     EMGRYLIIGAMLAAALQTFIPQSSLLAIGSGPVTSVLVMLALAILLSICSTVDAFVAL
                     GFFGTFSFGSVLSFLVFGPMVDIKSVIMYLQVFRRRSVAYIVAIPFLMSLLAGILFNY
                     LQP"
     gene            31523..32650
                     /locus_tag="PLE39_03385"
     CDS             31523..32650
                     /locus_tag="PLE39_03385"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE67395.1"
                     /translation="MFKRLFGQILLHLGEMEEEFRLFHLRDDIATFIITAVCLLLANA
                     IMLRVDFILYRSSMDLFYLIIPLRSLQVFLSGAFLFLVYRTTQVQKFDMFVFTWLLLN
                     VIYFSLFNFTRPADYMATTFDLLFIFGLYLLSPLQLKQTLALSVSLSLGSLYVTYYYK
                     SGIPYFIVGSTTGAHLFIHMLGLASALQIQSFKRRAFQAYARERKAHEITQDLLRIDV
                     LTKALSRRYFFEQAQWEFERSKQLNLPLSVLIIDLDHFKKINDSNGHHAGDEVLSRFG
                     HFILHQKRSQDLFGRLGGEEFSIVLPEAKTKEAQNAARRFQEAWVDTPVEVDSKIIHS
                     TMSIGIAELSQSDRNFDELLNRADSMMYKAKQRGRNRVAHK"
     gene            complement(32661..32873)
                     /locus_tag="PLE39_03390"
     CDS             complement(32661..32873)
                     /locus_tag="PLE39_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67396.1"
                     /translation="MKKIDRRGKLQENPFTFKETADGKVFIYWHGRLVKTLKNGAAEK
                     FLQGLTDENAQLRMARVTGNFKRGNE"
     gene            complement(32885..34429)
                     /locus_tag="PLE39_03395"
     CDS             complement(32885..34429)
                     /locus_tag="PLE39_03395"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNE67397.1"
                     /translation="MQLSPPSITYSSFLLIASITCLFVAVIVSRSRKNAAGANSLIAL
                     LLALAWWDATYGLFWAGTPGLTEYFWLDITYVGAVIVPGALFSFALQITNRQEWLKRP
                     FTLILYLEPLLVLTCLFTDPYHGLFFAGKRAENSAVILDAGPVFWANVAYSYTLILVA
                     TALIVGGFIRASGIYRKQLGLVAVGIGVTWLNSIVFIVGLNPLPGADNTPFSFTVAAV
                     AFAFAVSRYHLLDLIPVARDILIEKMRDGVLVIDSHNRIVDMNPAAQNLLNINSDKIG
                     KPVEEVIKKWQHYEKNTLPQIEMEIRRKTKRYVDMQTSFIVDDKGRNIGRLIILHDIT
                     KLKKVQSELHLLANSDSLTGAANRGYFMSLARKEIRRALRYKRKLSLVLMDMDFFKKV
                     NDTYGHASGDHALIALRNICINHIRKEDTFARLGGEEFALLLPETSQKTALHLAERLR
                     EALASTVINVDTLKFKVTISMGVTEFGIQEQDTFEGLFHRADKALYKAKADGRNRVVK
                     WHKGLG"
     gene            complement(34566..34982)
                     /locus_tag="PLE39_03400"
     CDS             complement(34566..34982)
                     /locus_tag="PLE39_03400"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNE67398.1"
                     /translation="MKSYRASISINASPEKIWKILSDASSYPAWDLSMDHIDGRLALG
                     ETVTFFTKLSAQSFPVKVTVFEPGKKMVLTGGLPLGLFKSERTHTLTPSKDGGTDFHT
                     EEIFSGLLLPLFGRNIPDLTSNFEGFVSALKRQAEK"
     gene            complement(34995..35354)
                     /locus_tag="PLE39_03405"
     CDS             complement(34995..35354)
                     /locus_tag="PLE39_03405"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNE67399.1"
                     /translation="MKYAKSKNDIVNEYIGELDVEIAPLFLEIRACVLAAGPVLNESI
                     KWKNCLVYGTTKNHIQTVVGKGRVSLIFFEGATLHDKYGLLEGDGSKTRTMRIASMDF
                     NKAALKDYIKQIMKNEK"
     gene            complement(35377..36093)
                     /locus_tag="PLE39_03410"
     CDS             complement(35377..36093)
                     /locus_tag="PLE39_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67400.1"
                     /translation="MESPMAVMSVEHFIPEPFFDYLFIQKGQVHIVDGGAGRKSALPH
                     QSLRNLYTRPVKLFFSTPLVLFGARFSLKFAESFSGEIKANCFLKQDWVKRDTNDLEA
                     FTLQVMEHVRMQQHPKLPHPILSLNFKESDWLDNFSARHKRRLYQKVFGLSRKDLQNI
                     QNVHVFLGQVCDFSARNPRIIEHVTPDVFYDQPHLNRSFKKMTGLAPVEYFQNSSILQ
                     DNLMSASYNEVLPAVDKMGI"
     gene            complement(36162..36758)
                     /locus_tag="PLE39_03415"
     CDS             complement(36162..36758)
                     /locus_tag="PLE39_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67401.1"
                     /translation="MDLEAQKALQVELDAADVRWAASAAHASIEKWSQRDGYYNNRLA
                     SHLTGKLGELATEKYLLENRFKLDSHFRFAERENLCDIVVKVKNYSQVCRLEVKTWSA
                     SYWAELGRCIAVDQYPTLKKKADLVIWCVLAPVDMDQLTASPSPLVVSLAGWSTMAEI
                     SKAPIHATGSGGMRKVTNYQLEEKSLHPFVEFGDELEH"
     gene            complement(36778..37779)
                     /locus_tag="PLE39_03420"
     CDS             complement(36778..37779)
                     /locus_tag="PLE39_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE67402.1"
                     /translation="MTTTHQRKNLGYWLSRIGRILLILLIIPLPIGWVWQVIAQRVEG
                     RQNLPLGQLTNVDGHMMHIYCTGEGSPTVILEGGVPEWSIHWQKVQPEISKFTRVCSY
                     DRAGYGWSEAGPNPRTAEVIVKELHTLLRNSGETGPFVYVAHSFWGPAALLYQNNYPD
                     EVVGMVLIETWSPDLFIPVPDVITQSLPLAQTLKAIAPLGQVRLVGELGILPLSEMLQ
                     AGLLPEELQPAYKAAYYDDGMWSAVAGEYSAMEQSGIQTQGLVSLADLPLVVVKAGIR
                     PADDYPPDEIWDTTQDYLASFSSRGRLVVVENSGHFVQLEQPALIVDLIRQVVEETK"
     gene            37970..39958
                     /locus_tag="PLE39_03425"
     CDS             37970..39958
                     /locus_tag="PLE39_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE67403.1"
                     /translation="MHGYGWFMAMRQGDEKPKVTRELLLRVLNYAKPYWWHITGMLVT
                     ILLSTGLSLLTPLIFRNMIDVVLPAKDTQRLIWLGLALLFIPALNGAISVVQRRLNST
                     VGEGVIYDLRASLFSRLQRMSLRFFTNTKTGELMSRLNSDVVGAQNAISNTIVNIVTN
                     LIEAIALFAVMISLEWRLTLVSILILPLFIITAQRLGTVLRDIARQAMDTNAQMNAHM
                     NETLNIGGALLVKLFGRTREEEKRFRERAANVRDIGIRRAVIGSTFFVIFGLVTAVGT
                     ALVYGLGGYFVITDVFTVGTIVAFGSYLGQLYGTLQGLASAPVDFSTSMVSFERVFEI
                     VDLPQDIAEKENAIELQDVQGELEFKDVTFNYKVDESILLKDVKRYGSMDNVKAVISG
                     QPSVDGKNSQGGNGSSSRIKNREGRNAASDSPEPDSTSQARDIALENISFVARPGQLI
                     ALVGPSGAGKTTVTYLIPRLYDPTDGIIRIDGHNLKDVTLDSLAHAIGMVTQETYLFH
                     DTIRTNLTYAKPEATQAEIESAAKAANIHNFIMDLPEGYDTIVGERGYRLSGGEKQRI
                     ALARVILKNPRILVLDEATSSLDSESEALIQDALKRVMAGRTSIVIAHRLSTILAADL
                     ILVMDRGKIAERGTHEELLAAGGIYSQLYETQFRGERV"
     gene            complement(39988..40440)
                     /locus_tag="PLE39_03430"
     CDS             complement(39988..40440)
                     /locus_tag="PLE39_03430"
                     /inference="COORDINATES: protein motif:HMM:NF019241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE67404.1"
                     /translation="MHPFFSDYLNLLQKCHNDIHAVLNDLPAEALDWGPGQEMNSICV
                     LVYHLTGAERYWIGDVAVGESSNRDRAAEFKVRGLGMDFLKKRLDDSFDYAASTLERL
                     SLQDLDRLCVSSKGECTVAWALLHALEHSTLHLGHLQITRQLWEKLAG"
     gene            complement(40442..41260)
                     /locus_tag="PLE39_03435"
     CDS             complement(40442..41260)
                     /locus_tag="PLE39_03435"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNE67405.1"
                     /translation="MKHISLDHPLARGRTADVYKWDDGYVLKLFHHWFPLEDIEYELR
                     IARAVHASGIKSPVAHELIRVNGRNGLVYDRVNGESMAAMLQHKPWKLFACAKTLAQL
                     HARMHDFVFGADMPKLRGKLQRKIENAPALPTPLKTALLTVLHSLPESDCVCHGDFHF
                     DNVLLTESDPVIIDWMDTSRGNPLADVARTSIILLGIVENREMHNPALKFLVRVFHSI
                     YLREYFRLRPQGRAEYIRWLPIVAGARLSEQIPELDGWLTAKAEEIYQYIGMEQ"
     gene            41334..41906
                     /locus_tag="PLE39_03440"
     CDS             41334..41906
                     /locus_tag="PLE39_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE67406.1"
                     /translation="MVKIENSKKTILNLAEALLQDKGFNGFSYAHIASELGVKNAAIH
                     YHFPTKEDLGQAVIQRYRDRFKLWVNNSRVKDLAPQEKLDWFFSIYTAMRSDQGKVCL
                     VGSMEVEFNSIPEGLQAEVQAMHKELLAWLEGTLTEGKAAGVFRFNGEPANKAALILS
                     TLQGGLQMARALGTKKFRDVIEQLKLDLLA"
     gene            41959..42771
                     /locus_tag="PLE39_03445"
     CDS             41959..42771
                     /locus_tag="PLE39_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE67407.1"
                     /translation="MTTLESTATKYQGIVKKAYQAFFSPSQYEVKSADREIIARGNNY
                     RISFEGGELAVTTWGESGPAVLLMHGWGGARAQMTGFVEPLLAAGYRVVAYDQPAHGE
                     SDGKMTNILEITPTMDLVEKREGKFDAIIAHSFGTLITSYALVHRNFPPPTRLVYLGA
                     FNRLLDSLPRFQVLANLPDEIIEGMRNMIYDNFGRGELDSIINESMVQQIHIPALMFH
                     DASDNVTPVDDSRAIASTWKSAQFIETSGLGHRGALQSKEVHAQIVDFLQKK"
     gene            42896..43228
                     /locus_tag="PLE39_03450"
     CDS             42896..43228
                     /locus_tag="PLE39_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HNE67408.1"
                     /translation="MKTALIFFFAVVSEVTATTALKFSEGFTKFVPSLIVVVGYGLSF
                     YLLSLSLKVIPIGTAYALWSGIGIVLTVIVGIVFWRETMDWARGIGIFLIMLGILVIN
                     LFSNSIAH"
     gene            43324..44295
                     /locus_tag="PLE39_03455"
     CDS             43324..44295
                     /locus_tag="PLE39_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HNE67409.1"
                     /translation="MNLRDLLRITSRTFAIGIERLPRVLCDATTIAYLLLRVSDYLED
                     NEEMEADKKVELLNLWVNILREEEAVETLTIQLTEANTENPDAIVAQHSKEILQHLRS
                     LPYEVQEIIVHHVINSTQGMARWTQHGSHLTDEKDLDDYMFEVAGRVGYLVTQLFAWY
                     SIAIRIKEKEIMPLAREFGLGLQTVNVIRGLREDFNRGWVYVPQKFLSEMGLTKEQLF
                     DPQHSEQALKVLDMMTDKAERHLRNALQYVKSLPWWLHGIRLGCIFPLMFAIRTLAVC
                     RHNTQVFESEAKMTRNEVKKIVMDSTLWGWSNLWLDRYYQELNVVGE"
     gene            44417..44875
                     /locus_tag="PLE39_03460"
     CDS             44417..44875
                     /locus_tag="PLE39_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNE67410.1"
                     /translation="MRLSKRGEYGLRAMIALAEPAEKGGAPQMMQIKEISLREQISPK
                     FLEQILLTLKNAGLLHSKMGVGGGYYLARSASEITLGQIFRVLDGPVAPIKCVSQMAY
                     EPCGCPDEETCGLRLAMGDVRNAIADILDNTSLADVTKRQSTVRKKLGIK"
BASE COUNT        11263 a        11857 c        11284 g        10437 t
ORIGIN      
        1 cattgagttg ggatgcgatc tgttcagcgt gggtcatagg ggtggaattt tatcagaaag
       61 gatgaacgct gcgcgtgaag ggggaaggat gaatatgagg ttgaaggtca aaggttgaaa
      121 gttgaaggac ggtagagtag tttacaaaaa cagcgcacag agattgctct ccatgcgctg
      181 ttgctactgc ttactgttta ctgctcactg ttctttacca aatacacccc tacaacgatc
      241 atccacacca gccacaggag actaccgtaa agccctgccc agccgatgac ggggaaactg
      301 ggaatggcgg tggcgaggag ttcggtctgc gcgaggatgt acacggcgga ggcgatccag
      361 ccgaggtagg cgacccattt gctgaatcgc ttcgatttgt aaagaatgcc actgagcatg
      421 gacatccaca agatgatgag gaattgccca agatgttcac cgaggatgac tccgccatac
      481 tggtggacgg cggaaaaaac aaggggcagc gctgccttcg tgaccggatc cgttgtcggg
      541 tcggtgaaga ggcgggcgag aacggggacg acaaaaaccc agcgcagcaa tccaaccact
      601 tgcacgatga atccgatcac gccgagagtc gtcgctgttt cgagaaaggg agcattttca
      661 tctgcgagga tgcgtttgag catgatcgcg ccgaggagca tgggcaggcc aacccatgca
      721 aaggcgagcc aggtataaat cagggagctg ccgcctgctt gaaatctggt gaggatctcc
      781 gcggcgggca ggcgcaggat atcgggatag tcgaagttga tgatgagcag ggtgtacgga
      841 atattaacga ggatcgcgcc gacgatgaaa aatataccgg caagtttacg aaaagttttt
      901 tcagacatgt gtttaccttt ttgaacgatg aacaaaataa gactgtgttt atggttgagc
      961 aatatagggt tcgagcgatt gcatcattaa ttgaacgccg cgttccatct gtgcttcggt
     1021 gacggctttg ccgcctattt cgaggctgtt caaccagaat tcggttatca gccagcaggt
     1081 ctctgcgagg ttttcgatca ctgttgggtt tgcagcgcgg agtacacctg ccgaggtgaa
     1141 ggctgcaaaa agctgggtga agccgtcgaa cccgcgcttg cggacggaaa gaaagcggct
     1201 gcgaagttca ccatcggcgc gtaataaggc ggcgagttcg cggtgggcaa agcggaattg
     1261 ccagaggatc ttgtaatttg ccttcaccat gttttgcagg tcagcaagtg tgggaagcgt
     1321 atcggcggag aggctgaagg cggcgtcatt agcggcaaac aagcgctcga ataactcgtg
     1381 gatgatctcg tctttgttgc ggaagtggta atacagatta ccggggctga ttcccgccgc
     1441 ttcggcaatg tgattggtgc tgaccgccgc cgtgccttga tcgttgaaaa gggtcagggc
     1501 ggtatcgagg attttttcgc gtgtggacat gggatcatct cctagagtat ttactctatt
     1561 attttagagt aaatactcta atttgtcaag ataaaaacag ggacggaatc cgtccctgtt
     1621 tttagttggt atcaggatgg gaaaattttc ttgaacactt cggggcaata cttctgcacc
     1681 atctcagacg agatgccgtt ctctttgcag atctcggcat acaggtccac gcttgggcgg
     1741 cggatgcgtt tttgggtatc gaggtcaagc ccccacaggc cgaagcgctg cgtccaaccg
     1801 cgctcccatt caaaattatc caccagtgac cagtggaaat accccttcac ctgccagttg
     1861 aaattgacgg cacgccaaac ctgatgcaaa tgctgtgcca gatagcgcgg ccgcatgtga
     1921 tcgtcggaat cctccacacc attctcggtg atgatgatgg gcaaattggg atatgtacgt
     1981 tcgatccatt tgatggagtt gaaaatgcct tcgggaatat tggcaatgaa gttcgtgtcg
     2041 ctcatatcgg cttctttggg gtagccgctg ttggagaata actctcccgg tttgaagaga
     2101 tcgaaccaga ccgtatccac cgagtagtaa ttcaagccga gataatcctg tgtgcctttg
     2161 gcttcaggga tgttttgatt tccaacagga gaacgcatca cacccgtgga aatggcagaa
     2221 gggaacccca tgttaattcc gttataacgg atgttgcgca tgagcctgtc gagcggcgac
     2281 caactcaaac gcggaaccat cggacggtaa tgcaaggcat agcctacacg cgattcgggc
     2341 tgcaattcat ggatggcacg atacgcggca gcatgtccgc gcagcatgtt tccaagcaca
     2401 cgcatggata gacggatatc cttcttgccg ggtgggaatg cgccttgcac gtacccgccc
     2461 aaagcataga cgttcggttc attgatggta caccaaagcg tcacatattc cttcaaagcg
     2521 ccgaccacct tgcggacgta tttctcaaac agtgggacga tctctgcatt ttcccaggct
     2581 ccgcgttctg tcacccaaag cggatctgta aaatgatgca ggctcaccaa agcggtcatg
     2641 ttgcgttcac gcaatccgcg cagcatggtg cggtattttt cgatggcttc ttcatcccag
     2701 gtatcgggcg tgggttgaat gcggctccat tccaaagaca ggcgatgcgc gttctggcct
     2761 gtctcggcgg cacggtcaaa gtcttcgcgc cagcgtccac cccaccaatc ggcggcaaga
     2821 ccagatttgt ggacggtgtg ccccgcttcc tcccatgcgt gccagttgtt attggtgttg
     2881 ttgccttcca cctgatgtga ggcggtggcc gttccccaca gaaagccttt tggaaaatga
     2941 taggttgctt gaggcatgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
     3061 aacagcagca ttgagcgatt cgatcctgcc tttcatgggc agcttcaaaa cgatgtcgca
     3121 tttctttcga acgaggtcat gcaacccttc gccttccgaa ccgacgacaa gaccgagtgc
     3181 gcctttgaga tgacgagatc ccgcctccac ctccgcccca gcctgatcca gaccgacgat
     3241 ccacacatca ttatctttga gcgcatcgat cgtctgcgaa agattcgcct gtgcgatcaa
     3301 catatgctcg ctcgcgccag aagatgcatt gaccacggct ggggtcacat ccaccgagcg
     3361 cgccagcgga atcaccacac catgtacacc gatggcctcc gccgtgcgga taagcgtgcc
     3421 gaagttctgc gggtcctgca acgaatcgag gatcaaaaca aaaggcagtt cttcctttgc
     3481 ctgttcgagt atctcaacca catccgaata cggatatccg cttacttcag caacgacacc
     3541 ctgattgttc tgatggacct tgtccagctt ggggcgtggc acgcggctga tcctgatttt
     3601 ttgctgctgt gccatcttaa cgatctctgt caaacgccct ttttcctgca ccccttcggc
     3661 aatttcaatg gaaaacacct gtctgcgttt tgcgcgcagc acctcataca cggcattgcg
     3721 ggaatagata agttctttca tggtggattg attttacatg agaaacaact cttctgtgaa
     3781 tgtataattt tcagcatgaa aaaagagttc accatcgcca cgatcagcac aaaagattcc
     3841 ccatttataa atgacctgtg caagcttcgt gatggattat ccgcacacca tgacgacaca
     3901 acgtcatccg agcgtttcag gcagttcatg ctcaagcacc tcggcgaaga aaccatgcta
     3961 acgttcttga tcctcaacaa cggccgaccc attggctacg gccttgcttt cgatgtcatt
     4021 gaacacgcct acatgcccga atggacccgc aagggataca ttacgcagtt cttcgtcaac
     4081 gctgagtatc gcggtcaggg catcggtgaa atggggctgg cctacatcca cgattggttt
     4141 cgcgcacgcg gcatcaccga tgtgttgctc aacgtagcga tcaaaaacga agtcggaaat
     4201 cgcttctggc agaaaaacgg ctacaccccc tacgccacac gcatgatctg ccgcctcaaa
     4261 tgatcactct ttatacgagt tgatattatc cagcaaatcc aacaccaatt cttttaaatc
     4321 gccgcgattt ggcagtgaac cgatcagccc atacatggga gccatacgcg caggcaaacc
     4381 gccgcctcca ccaagaagcg acgaagcctt gtccatcaga ttgctgaccg tctcctcagg
     4441 caggactctg gtcaacaggt tggcagcctg gaccagaaac ttcgtcgcgg tctttcttag
     4501 cccgggcctg cggactcttt cagcggcatt cgccaggtct gtgagaaact ccgcccccac
     4561 ctgtgcatgg acgtaattca ccgtcacatg cagagacggt ggaaagatct gcctgtccaa
     4621 atgccagccg cgcagggcga gttcatctgc gagttcgtac acgtccgttg aatcagatgc
     4681 gatggcaaag acgctcatct ccgggttgct caacaccttc aaaccgggga tctcattgac
     4741 gccatcctgc aagatttttg ccgtgcgcat gacgatatca gtgatctcaa gataaccatc
     4801 ttccccgaga taattgagca acgcccacgc tgcggcaaca gggccgccgg ggcgcgaacc
     4861 cgccatggat ggagaaggat aaatgccgcc cggccaatcg gtggaaacaa acatttgatg
     4921 gcggcgcagt tccgaggtcc gataaagaac gagggaagcg ggtttggcag cgtaggcata
     4981 cttgtgcaaa tccgctgaga tggaggttac accgggaacg tcaaaaccaa actcaggcac
     5041 agggtatccc aacttgcgga cgaagggcag catgaagccg cccacacagg catctgtgtg
     5101 aaaaagcaga ccgtgttcct gcgccagtgc agccagttca cggatcggat ccaccacgcc
     5161 atgcggatat gagggagccg aaccgaccaa catgatggta ttcggtgtga tggcgcggcg
     5221 tgtggcatcc acatcagcgc ggagatcttc acgcacggga atgtgaatca tgcgcacgtt
     5281 gaaatattca cccgccttat ccagtgcagg gtgtgcggtg ctgggcatga tgatctctgg
     5341 tttggtgatg cctcgctttg cacgcgcata atcacgcgcg gttttgaccg ccatcaacaa
     5401 agactcggtg ccgcccgagg tcatggtgcc tgccactttg tcatcgccgc ccaacaggtt
     5461 ggatgccatt gcgatgatct cattctcaaa tttgctcagg ctgggaaagg ccgtggggtt
     5521 gagtccgttc tctgaaaaga acatggtgta ggcttctttg agcaggtcgt caatctcttc
     5581 tgagatgtga tagaccaggc caaagacctt cccgttttgc cattgggcat ctttatcccg
     5641 tgcgcggcgc atggattcga gaatatctgc cttgggtttt cctgatttgg gaagcatgag
     5701 tgtaggcatg aaggactcct tttaaaaaaa tagggacaca gcggcgctgt gtccctacaa
     5761 tatcacaaca tttatttttt aaccccagcg ccaggtcgtg ccatctttgc tgtcttcgat
     5821 ggtgacaccc atctctttga gctggtcgcg gagttcgtcg gaacgtttcc actgtttttg
     5881 tttgcgcgct tcagttcgct ctgcgatcag ggcgatgacc tgcgcctcaa cttcgctgga
     5941 gcctttcttt tcttcgagct tgaggccgag gactcctgca agttcaccga acatggtttg
     6001 ggcggccttg agttgatcgc ctgtggcgcc gttatcgcgg gcggtgttga tggctttgct
     6061 tagttcaaac agcgcggcga cagcacctgc ggtgttgaag tcattgtcca tggcttcggt
     6121 gaagttcgct tttgcagatt cgctggcaga ggtgagagcc gaagcggctt cggcgctgag
     6181 tccctgtgag gaggcagaag caggccgcag ggcggatttg aggcgctcga cgttcttttc
     6241 ggcggcgtcg agtgtatcct cattaaacat caacggtgcg cgataggtcc cgttcaagac
     6301 caacatgcgc atgacatcgg cggaacgcct ggaaaggaat tctttgatgc tgacgatgtt
     6361 gccaagcgat ttggacattt tttcgccgcc gagctgcaac atgccgttat ggatccagta
     6421 gcgggagaat tccttgccgg tgtagctctc gctttgggcg atttcatttt cgtgatgcgg
     6481 gaagatgagg tcgttgccgc cgccgtggat gtcgatctgc tcgcccagtt cggcgaggtt
     6541 catggcagag cattcgatgt gccagccggg gcggcctttg ccccacgggc tgtcccatga
     6601 gatttcaccg ggtttggcgg ccttccataa agcaaaatcc atgggatggt ctttggcttc
     6661 gccgatttcg atgcgcgcgc ccgcctgcat gtcttcgagt ttgcggcctg agaggcggcc
     6721 gtagtcgtcg tcgctggtga cacggaaata cacgtcaccg ttggtggcgg cataggcatg
     6781 tcctttttgg atcaaccctt cgatgaattg gatgatgagc ggcatggtct tgctgacctg
     6841 cgggttggat gtagcgggca atacattcaa cgatttgaga tcgttggcgt agtcttcaat
     6901 atagcgttgt gacaatttga gtgggtcttc gccgagctgg ttggcgcggt tgatgatctt
     6961 atcgtccacg tcggtgtagt tcatgacgtg tttgacggcg tagccccgat attcgaggta
     7021 gcggcggatg atgtcgaaga caagcgccga catggcatgg ccgacatgag catcgttata
     7081 aaccgtcact ccgcacacgt acattttgac gaggttgggt tcgagggttt ggaattcttc
     7141 ggttttgcgg gtgagagtgt tatagatttt caacatggtt ctattttaac cacagatgaa
     7201 cccagatgaa agggattaat aagggcaaaa taaaacaggg cgcctctcga cgccctgtta
     7261 ccgaccactt tcaccaactg cttgttattt acttgccttt gccctttttg ccactgttct
     7321 cttcatccac gcgtccaaag atcatacggc ctgcggcggt ttgcagcacc ttggtgatgt
     7381 tcacttccat gtactcgccg atgtaatctt tgccgttctc caccacaacc atcgtaccgt
     7441 catccatata gcccacaccc tgaccatatt ccttgccttc ctgcatgatg ttgatgcgca
     7501 gggtttcgcc gggcagcacc accgatttga cggcattcgc aagttcgttg atattgagca
     7561 ccgtcacacc ctgcaattcg gcaatgcggt tgaggttgta gtcattggtc agcaccgggc
     7621 tcttgagttg tttgccgagc acgatgagtt tgtcgtccac ttcacgcacg ccttccacat
     7681 tgatatccga aatgcggacc agcacgttcg ggagtttctg caattcagag agcacttcca
     7741 tgccgcggcg tccgcgctga cggcgcatgc catcggggga gtctgcaatg tattgaagct
     7801 cgttcaacac aaaacgcgga atgagaagcg tgccgggcag gaatccagtt ttggcaatat
     7861 cggccacacg accatcaatg atgacactgg tatcaagcag gatgttgcgg ttgagattcg
     7921 tccacgagga agaaccgccg ccttcaccac gtccacttaa tgcactgagc agtcccataa
     7981 tgtcaccttg acgcatggtg aacagggaaa tgccgagata agaaaagacc agcaccccaa
     8041 gaaacggcag gatctgacca aacggctccg gcaataacga aagcggaaag cccaacagcg
     8101 ccgcgatcag cagtcccacg atcagtccgg tgagggccgc aaacaaactc tctgccgcaa
     8161 gcctgccaag cagtgagcgc aagtaacggg ccgggcgcgt ggtaaagtat ggagtgagaa
     8221 taatgccggc caaagctccc accaccgtgg aaagcgtcgt tgtcacaatt gggtctgttt
     8281 cagggtttgc cttcgtaaaa ttgaaccccc acatcccccc tccgatcgcc agcgcaaaca
     8341 ttgccaaaat acgaagaata aattcaaaac tcataataac tcctttataa tgaatgcaga
     8401 ataataaagg ataatagcac gggtaccacc caccgtcaat cacccccggg aagattcata
     8461 tattattgtt ataatctgga aaataccatg gcaatagacc gttttggacg aaatattcac
     8521 tacctgcgca tcagcctgac ggatcactgc aatttacgct gtgtatattg catgccggag
     8581 gatatgacct tccgccccaa tgcagacctg atgcaggacg acgagattct aaccttcgcc
     8641 cgcctgtttg ccagcctcgg cttcgataaa atccgcctga ccggtggcga accgacggtc
     8701 cgagccaaca tcgtggacat tgtccgcggg atctcctcca ccgagggcat ccgctccgtt
     8761 tccatgacca cgaacgggct cctgcttaaa aagctggccc aacccctggc agacgccgga
     8821 ctgcaacgcg tcaacatcag cattgacaca ctcaacccga acaaattcaa acggctcacc
     8881 cgctggggca aagtggagga agtctgggaa ggcatccttg ccgcagagcg ggcaggcctc
     8941 accccagtca aactcaacgc agtggtggtc aagggctaca acgaagaaga tgtcattgac
     9001 ctggcagcac tcaccatcga ccacccctgg cagatgcgct ttattgagat gatgcctttc
     9061 ggcggtgcaa ccgacctgca gatcaatcag gtcatcacca tgaaacagat ccaagacaac
     9121 atcgaatctg aatttggaaa gcttgaatcc gccaacgacg gcaaactcga cggcgaagcg
     9181 caggtcttcc acatccgcgg cgcaaaagga aacgtcggtt ttatttcctc ggtcacgcac
     9241 ccattttgtt ccgcatgcac ccgtgcccgc ctcacctctg acggaaaact gcgcctgtgc
     9301 ctgctgcgcg aaaaagaagt ggatctgctc acacccatgc gagctggagc aagccttgaa
     9361 gaattacgca gactcctcct ggatggggtc tgggaaaaac cctgggggca tggcctggca
     9421 gatggagtca ttccactgaa tcgggtcatg agcgaaatcg gaggatagca ttaacaacat
     9481 caaagcaatc ttgttcgatc ttgacggaac acttcgtcat cacctcccct ctggcgggga
     9541 ggtttttgtg gaatttgtcc gcagcatggg cttcaaggtc tcagaagaag atcgcatccg
     9601 cgccgaacac tgggagcatt tctatttcgc ctcgtcactg gaaattcgca aagatagcga
     9661 aacctataaa gatgatcgca gcggtttctg ggtcaatttt gggagaaggc gtctgatcgc
     9721 attgggcttg aaagccagcg atgcccttga ggtggcgccg aaagcatctg ctcacatgaa
     9781 cgaagcctac aaacctcagg tgcatgttcc gcccgatgcg catacccaat tgacggcatt
     9841 gaaagaagcg ggctacattc tcggcgtggt atccaaccgc gaaacgccct atcaggatga
     9901 gttgaaggaa ttgaaactcg attcctattt caaattctcg cttgcgggcg gcgaggtgaa
     9961 ttcctataaa cccgacaaga tcatcttcga gcgcgcattg gaactggcgg gaacctccgc
    10021 agctgagacg atgtacatcg gcgacaatta ttttgccgat gtgatgggag cgcatcgctc
    10081 cgggctgaag cctgtcctct acgacccaac caacctcttc cccgatactg gcggcttcgc
    10141 gttcatcagg tcatttgctg aactacccga gttattgaaa taatcaaacc acccaacacc
    10201 gcgaatccac aaacgattcg cggtgttggc gttaaaatga agatggaaca cttgaaagac
    10261 aaatgacaaa ggagtccaac atggcttggg aacgaaaact cattcgaacc gtgcgcgttc
    10321 gcaattcaca aaaaacgggg gtgctcgccc gattattgac cgccattgcc gaagcaggcg
    10381 gaaacacggg cagtcttgtt ctattaactg aaacatcaca gaacgtggtg cgcgacatta
    10441 ccatcactgc agaaagcgaa gcgcatttcg agaccattct ggatgccatg cgaagcaatg
    10501 aaggcacccg catcatcgaa atacgcgatg aagtgctgga actccatcaa aaaggcaaga
    10561 tcgccatccg ttcgcgttac cccatcgaat cgctgaccac cctgcggaga gtctacaccc
    10621 ccggtgtggc tgaggtctgc cgcaagatcg ccaaagaccc ttcacaagcc cgtctgtaca
    10681 ccagcatcag ccacatggtg gccattgtta cagatggaac ggccgtgctc gggctcggtg
    10741 acattggccc gctggcaggg atgcccgtca tggaaggcaa agccatgctg atggaaacgc
    10801 tcgtcggtct ttccggaatt cccattttgc tcaacaccaa agacaccgaa gagatcatct
    10861 ccacagtggc tgccatttca ccgacatttg ccgccatcca actggaggat atttccgcac
    10921 cacgctgctt cgagatcgaa gaacgattac aagccatgct cgatatcccc gtcctgcacg
    10981 atgaccagca tggtactgcc gtcgtttgta cggcggccct gctggtggcc acccgcgaaa
    11041 caggcacaga cttgaacaag gctgtcatcg ggcagatcgg ccttggagcc gctggtcatg
    11101 ccatcggtgt gatgatgatg cgtctgacgg gtaatccagt gtacggtgca gatctaagtc
    11161 ccgaagcgtt ggagcgcttt gaaaaggcgg gtgggaaaaa atccaactta agagaagtca
    11221 tggaaaaatg tgacatcgtg atcgccacca ccggggcgcc gaatctcatc aaacctgaaa
    11281 tggtgcgcaa gggacagatc atccttgccc tctcaaaccc caacccggag atcgacccag
    11341 aggttgccat ggaacacggc gctgcatttg ccgcagacgg gaaatcggtg aataatgtgt
    11401 tgggcttccc cggcatcttc cgcggcgcag tggatgcaaa cgccccgcgc atcacacatg
    11461 aaatgctgct ggctgctgca catgtcatcg cagagatgac accgcccggc gaactcgtcc
    11521 ccagcccgct tgataaaaag gtacatcggg ccgtggcacg cgccgtggca gaaaccgcca
    11581 tcaaacaagg cattgcccgt gccgaatacg tgccctatgt ggaagaataa agatcaaaga
    11641 taaagacaaa agacggcctg cctttcgagc aagccgtctt ttatttttcc actttctatt
    11701 ttttacttcc cgttcttcaa cgcctctttc aaggtcaagg ccaggcgtga aattccctcg
    11761 cggatgttct ccgggctgga aaatgagaaa ttgaggcgca tggtattttt tccgccacca
    11821 ttcgggtgaa aggccgcacc ggggacaaaa gccactttac ggtcaatggc caccttcaac
    11881 acttcagccg cgtccacatt ttcgggcatc atcccccaca ggaacatgcc gccctggggc
    11941 tttgtccaac gcacgtcaga agggaacaat tcatccatca actcaagcat cacatcgcgg
    12001 cgttccttgt aagtggcgcg gatcacttta acatgttcgt ctaaaaatcc gcctttggct
    12061 acttcatagg ccacatgttg attgaacgat gacgtgtgca ggtctgcagc ctgcttcgtc
    12121 atcaccagcc tgcggatcac ctgcggcgga gcgatcaccc acgccagacg caggccaggt
    12181 gcaagcagct tcgaaaaggt gctcaggtaa atgacgttcc ccgtgtactc gccattgtct
    12241 tcattgcgat aacggctgtc gagggtcgcc acagatggga tatgttcgcc atcgtaacgc
    12301 agctgaccat agggatcatc ttccacgatc ggcacaccgt actggtctgc cagcaacacc
    12361 agccgtttgc ggcgctcaag gctcattgta gagcccgatg gattttggaa atttggaagc
    12421 acgtaaataa atttcggccc gattcgcagc gcctcttcca attggtccac gatcatgccg
    12481 tcttcatccg aaggcaccga aatatattgg gcgccgtagg cgttccacgc ctgcaacgcc
    12541 cccaaatatg taggcgactc caccactacg taatcgccgc gattaatgaa caacctgccg
    12601 ataaaatcca gcgcctgctg tgaacccgaa gtgatcatga tattgtcaga ggtgacccgc
    12661 acactgtaac gcgcattgtg ccttgcaatc atctcacgca atggtagata cccttcggtg
    12721 gtgctgtact gcaatgcctg cggcccatga tcggtgagta cctgattaca ggcctcctga
    12781 aattccttca acgggaagac ctcgggggcg ggcaatccgc ctgcaaacga aataatatcg
    12841 ggctgctcgg taaacttcaa cagttcgcgg atgaccgaac ttcccatttt ttgtgtacgg
    12901 tgggcgtagc gatactccca gggtgtctgc attttttctc cttgatgaac ggaataaaaa
    12961 agcctgacag gtacgtgtgt acccgtcagg ctggctgact taaggcatta atatgcccaa
    13021 aaggtcgaag gccgctattc tttccatggc acctttatct taaccatttt caggcgggac
    13081 tgcaacctca ttttgtatga aacgccttat gacaaatttc ctgcaatgat atgctcaacc
    13141 gaagtccgcg tgtaagaatc aaggggcaga tgaagagcct cgctcggact cacccaaaca
    13201 tgatcctccg cctcatcatt tagctgcacc gactctgaat ccgtcctgca ggcgtaatcg
    13261 aagaagataa aatgccgccg cttccaaaac gaagggtcgt aaatgaactg ctgaaaattg
    13321 atgaacttca ggtcgtaaat atccagccca gtttcctcct tcgcctcgcg gatcgccgcc
    13381 tcctccagcc gctcacccaa ttccacgtgc ccgcccggga cgacatactt gcccgggaat
    13441 ttatggcttt gcagcaaaag cagttcccct ttcttgttga aaataaacac acctaccgtc
    13501 ggttctggaa agatctggtc tgccattatt cacctatgaa atcttcgcaa gggcgaatca
    13561 cccgaacact gcaatcacca tcacgcctgc aaaaacgatc gccgacccca ccacacgaat
    13621 gccgcccatt tcctctttca aaaatctcca gccgaggaag gcgccgatca cggcactcac
    13681 ttcacgaatc gccccggaat agctcagcgg ggcaaaggtg tacgcaaaca aagccagcat
    13741 gtaagccacc aacccaagca gcccgcccat caaaagatat cctcgttttt gccgccacat
    13801 cgctgaaaaa tgactccatc cataccgctg aaccagatac ggcgttgtca cgaacggaac
    13861 catcacaaac atggacaacc cgtacggcag ggctggtcca tgctttacgg cattgccgtc
    13921 aatgtaagtg tagatcgaga tgatcaaagc caccgaaagc gctgtgagga ttccacgcaa
    13981 gtggggcttt tcctcgtggt tttgcaataa ggtcgttgcg ccgatcagga tcattccgcc
    14041 tgtgatcatc gccaggccga tatacccgcc cgtggtcaat tgctcatgca aaaagagcgc
    14101 cgtccacaac acgagcaggg caggggccgc tccgcgtgca atgggataca ccagcgaaaa
    14161 atcatggtcg ctataggcaa tgcagagcaa aatgaaatag atcgcttcaa ggatcatgct
    14221 gatgacagca aaggtccaca tcgaacgcgg cggcaggccg gtgaagaaga tcaacaccgt
    14281 ggagaagaca ccgctcagga tcacctgcca ccccatggca atgtattttt cctccgcgct
    14341 ttttaacaaa aaattccacg tagcatgcat cgccgcggaa aagaacagca gcacaagggc
    14401 aaggatcggc atgaattacc tcagaaactc gatcagattt ttattcactt catcggcttc
    14461 atcgtgctga acccaatgtg tggacttttc aaaaagcacg gtttttccat catcgcacaa
    14521 tgcaattgac ggtgccacca tttcgtggct cagcgccaca tcccgttttc cccacagcat
    14581 caaagttggg atagtgattc gacgggcgga aatcttttta ccaaacacat gccacagact
    14641 acggcgaaac acggcacggt accaattcag catccccgtc agcgcaccgg gctgtgccca
    14701 tgctttttta tattccgcca ggtcttgtaa tgtgaatgtg ctttttcgcc ccgaccccac
    14761 caacatccga ccgagattgt ggaagtcgtt tttgctcaac atccactcca caaaaagcgg
    14821 aatttgaaag aagaacacat accacgattt tttgcgctgt tccgcattct tcaacacaaa
    14881 ttccgtcatc acatcggggt gcggcacatt gaggatggca agttttttca atcgctcagg
    14941 gtgattcagc gccaccgtcc atgcgacaac ggcaccccag tcatgcccga cgaggaaggc
    15001 cttctcgatg ccaaaatggt cgaacaagcc aagaacatct ctggcaagga catccacagc
    15061 ataggaggaa actccctttg gtttgtccga aaggttgtaa ccgcgctgat ctggcacgat
    15121 gactcgaaat ccggccgccg tcaacgcggg gatttgtttt ctccaaccgt agctgaattc
    15181 gggaaagcca tgcaataaaa tgaccggcgt cccattttcg ggtccgtcgg tcattacatg
    15241 caggttgatt gaattggttt ttacaaattg gctttgcatc gcgctagtat aacggaattc
    15301 tggctcctgt tactttatta gcttcttcag aaaacagata ctgcatggca gagacgatct
    15361 cagcggggga ggtgcctttg ccttcatttt tcacgtcaat tgactttacg tgaatgacat
    15421 tcgcagtcaa cccgttttcc ttgaattcct gcgccagcgt cagcaccaaa ctttcctgcg
    15481 cggattttgc ggcggcgtac agtgccatct tcgcagaagg attcaccgtc accggcatgg
    15541 atacggcgat gacccgtccc cacgcctcgc ggctgtttgc gatgtgtgga gcaaatgatt
    15601 tcagcaggtg ataggtcgtc cacacgtgct ggttgatcat cgcgtcaagg tcggctgcgt
    15661 ttgattcagg gatggtcttg ccgcccgtcc acccgccgac gagatggatc aaggcgtgga
    15721 cactgccgaa tttggaatga acagcttcgg ctgagtcctg aaccgcctgc ccgttgagca
    15781 ggtcaatggt ttgggcgtgg agtctttcag aaggcaggtt caattcacgg acgagggatt
    15841 ccagtttcgt ttcgtctcta tcaagcaacg caatgttgtg cccatccgcc gcaaatgttt
    15901 tcgcaacaag gtttcccaac acacccgtcg cgcctgtgat cacaattgtt ttcatcttca
    15961 acctttaatt tcaacgttca aacgttttaa cgttgaaacg tttgaacgtt tactcataca
    16021 gtgcttgaat ctgatcttcg gtcatgccag ccacaatcgg cgggtcgctc tgttccacaa
    16081 gatgcggctt gccgtggaaa tcgatcatgt gacccgattt ggcggctttg gtttcgaggt
    16141 agaagcggtt gtgttcgttg ctcggcaaaa tgtgcggcag gcgttcattg accttcacac
    16201 catgctgttc aagctgcgcg atcttcttcg ggttgttggt catcaaacgg atcgactgga
    16261 tcttgagcga gtgcagcatg tgggcggcta cggcataatc gcgttcgtca tcacggaagc
    16321 ccaatgcgag attcgcttcc accgtatcga ggccctgatc ctgcaaacta taagcgcgga
    16381 tcttgttggt aaggccgatg ccgcggcctt cctggcgcag gtagagcaca atgcccttgt
    16441 ccatttcgcc gatcttctgc agcgcggctt cgagctgatc gcggcaatca cagcgcagcg
    16501 acccaaccac atcaccggtc aaacattcgg aatgcaaacg cacgggcaca tcttcagcgc
    16561 caagcacttc gcctttgatg atggcaacat gttccttgcc atcgcggtta tttgaaaacg
    16621 ccacaatgtg aaaatcgcca aagcgtgaag gcagttcggc aatcgcttcg atatgaacgc
    16681 aaatcttatc cgagccgtat ccttcacatt cgtgacagca gtcttcttcc atgagaagct
    16741 ggattttttc gtgtgtaaaa gtagtcatgg tttcctccgt aacgcaagtt gatttctcac
    16801 gctacaatct gtatttcaga aggaccccgc cgtcctccca tttttcaaca tcgatcaact
    16861 tcaaacccat tgccacatca cgtggcaggc caaccccatc tgcaggggtg ggagcattcg
    16921 cgccgccaaa gatcatcggc gctacaaaaa tcatcaactc atccacaagt ccgaggcgca
    16981 tcaactcaaa attcattgtt ccgcccccct ccaccatcaa atggtccacg ccgatctttt
    17041 tcagcgtcag catcatcgaa ttcaagtcca cgcgcggggc atcgtgtaca aagacctcca
    17101 cgccgcaggc ggtcagttcc gcgagtttgt ctttagatgt ctgatttgtt gtaaatatta
    17161 cgacccgtga gggacctgct ttaatgaaat ctgaatcagg cgggatatct gcatttgtga
    17221 tgcgtgtgac cacccccacc ttaatcggat tcgggctcag cccacgctga accctgccct
    17281 cacgcagcgc ttcgctctta actgtgagct tgggtgcctc gtcgagcagc gtccgccccc
    17341 cgacgaggat tccatctgcc gcggcgcgca gctcatccac acgggtttta tcgcgtttgg
    17401 atgagatggc cgagcctttt ctttcaaagg tgtctatctt tccatccgcc gtcattgcaa
    17461 cgttgatgaa tgtaaatgga cgattcatgg aataacaatg ggtgatagac cgttatcttt
    17521 acccaattct taccgagcgc attgaaactg aaccacccca aaccttttct gcaaagaacg
    17581 acactttatc gtcatcatcc ttcaatgcca gcgaatacag cagcggaaca taatgctcac
    17641 cggagttgat cgccaattgc gcggcctgcc cctgcttttg gaaattgatg atcgggtcat
    17701 ggtcgttggc aagaatccag ttcttcacct gtgtgtcgaa ttccgtggcc cagtcatacg
    17761 caaaatcacg gaattgcacc atcgagaggt tgtgtacgat gtttccagag ctgatgatca
    17821 gcacgccctc ttcgcgcaat tccttcaact gcttcgccac ctcgtaatga tcgttgatcg
    17881 aaaggttggc attcaaactc atctggatca caggcacatt cgcttcaggg aacatttggt
    17941 tcaaaacaga ccatgtgcca tggtccaaac cccattcctg atcggcgtag acttcggtct
    18001 tcttgatgat gcgccgcacc tgttccgcca aatcgggcga acccggcgcg gggtatttca
    18061 cttcgtacaa ttccttcgga aaaccataca tgtcgtagat catgcgcggc tgttccatgg
    18121 cggtgaccag cgtaccgcgt gtaacccagt gcgcggaaac acacaaaatc gcgcgaggct
    18181 tgggcagcgc cttgcctgca tcacgccagg cgcgcgaaaa ttcattatct tcaatggcat
    18241 tcatcgggct tccatgccca acgaacagga ctggcatttt ttcggtcata tgacctcctt
    18301 agtgattggc ttttctgcga acatctttca aaatatcggc aaccgtgtaa ttgcccagcg
    18361 ccttttccat ttcagattgg gcatctgcaa atacagattg caacaccccg ccaatgttcc
    18421 cgccgatggg gcagtgtcta ttcgggtggc tgtggaccgc caaaacattc acctcatcca
    18481 aactgcgata gacctcgcac aagacgatct ctttcggctc gcgcaacaac cgccagccgc
    18541 cttttgcgcc tgatttggat tccaccaatc catgctcgcg caaactcgcc atcgtacggc
    18601 gaatgaccac ggggttggta tccacactgc tggcgatcat ctctgaagtc agcggatcat
    18661 tgcttgaggc aagcaaggtc aaagcatgaa tggaaatggc aaaacgttgg ttggtgttca
    18721 taattgtaac cattatagtt acgaatacaa aagagtcaat ggtcaggaca gaattcttag
    18781 gaaagattaa tcacctgaca caaggactcc cccatactca acggttaata cgattgggaa
    18841 aagtaagccg cgatcaaatc gctgatatct gcactggtgt tggcacggat cgctgcaaac
    18901 aaatcgaacg aagatgcacg tccgcgggag tgaattgcgg cgtaatcctt caaaaagcgg
    18961 aagaaattat cgtcacccat gcgaatacgc aggtcttcaa agaagtgtgc gccattcaaa
    19021 tacacagcat tgacatacga acgctggtca ccgaaattat aaatacttgc atccacataa
    19081 ccaccggcgc caaaataatc cacgcggaat tgccaccacc aatcgccata gcgcggatag
    19141 ttgtattcat aaaagatacg ctcactgtaa accgccatgg cttcatccac ccacggttcg
    19201 agtgcctgat cgttgcctac aagcccaaac caccactgat gcgcgatctc atgcacaccg
    19261 atggtcgtca gattgttttt ggcgccgccg ccatattggc tgtaaaaatc tgtggaaaga
    19321 aaaaccaacc cgtcatattc ctgcccatcg tgaatgtcag tctgcaccac tgcaaggctt
    19381 tgatacggat agggcgcaaa cttcacatca aagagactga ccgcctgcac tgcgccgtac
    19441 aggattccat ctgccgctgc gccatagcca tcaaaataat acgagcggat caccaccgac
    19501 ccgacagctg attcggtcac gaggaattca tcacttgctg aaatagagaa tgtgcgcgca
    19561 ccgtaaaggc ggtaacgggt ccattcaccg ttcggttctg aaggagcacc tgccgcgatg
    19621 accacaccgg cttcggtttt gatattcagt tcgatgtcag cggcatcata aacaagatgt
    19681 tcaccaaaag gcatggggtc gtgcagcacc cagcctccgt tatacggcac aacaaaggga
    19741 taccaatcta caagattgat ctggttgaaa tcatagccga acaaacccgc ccccttagca
    19801 gggacattga gcctgaagct gagtgtcagc gtggtggctg agcctgccgg gagtggctgg
    19861 ggtaggttca tggtcagagc ctgaccgttg agtgtgtaag tggtcaacgc aacagagtcc
    19921 tgcgaaatcg tattcaatac aaatgcgccc ccgtataaat tcggttgaac cgacaaaacg
    19981 atatccgaaa gggtcaaacc tgtgccattg tagtagcgga tcgtttcttc cacatccagt
    20041 gtgcgtgagg caaagtccat tgtggcatat aaaatgtaat tcgtacgcga aggcgctgaa
    20101 ggcggcgggg atgtgaaagt gactgccggg ggagggttgg tgggcggcac ctgcgtttgg
    20161 gtggggaatt gggtaaacgt tgccgaagga aggacggtgg gctgcagcgt cggtgtggat
    20221 gttggcaaca aagggacgct cgtatcggtc tgcgccacgg gttgaaaggg tgtggtggtg
    20281 ggcaatgtcg gggcgcgtgt tgccaaaatg aatggcgcaa agacctgcgg cgtgggggca
    20341 ggtgtaggcg tggcacagga tgcgagaaaa gaaaacagca atactacaga cacaatgcgt
    20401 gtgagacggg aacgaatggg gttcattaat agatattctg gaaatattgg cgcacgatat
    20461 cagaagtgtc ggtgagggtg tgttcgccaa gaatgcggaa gaaatcgctg gtggtcatga
    20521 tcttgttgcg cccctgcaag agataatcct gaatgaaggc gaagaaggca tcatccccga
    20581 tccgggcgcg gagttcgctg aggaaatgcg caccttgaaa ataagccgcg ttggtgtatg
    20641 gacggaatcc ctggccatca tagatgggaa tatccacaaa gccctgcggg ttatagaaat
    20701 caatgcggta agcccaccac caactgacaa ggtccgggtg aagggtttcg tagtagacga
    20761 gttccgaata agtggcgagt gattcatcga gccagggttg ttgggcctgg tcattggcca
    20821 cctgatcgaa ccaccattgg tgcgatgtct catgcaccgc gacgaagatc aaataattgg
    20881 ccggggtttc atcgtacagg ctgtaaaaat cacgggaaag atagaagaag gcggagtact
    20941 ccatgccatc gttgaaatcg cccattacca tcgaaagcgt tttgtgcgga tacggcccgt
    21001 atttttggct gtagatctgc aaggcttcgg cactggcttg taaagcggca cgcccggcga
    21061 tcttgctcag cggaaagtaa taactggcca cgatcacatc cccaacctgc aggcttgaaa
    21121 cttgaaactc cgggctggcg gcaagggcaa aggtccgcgc ggcggtgatg gtataacggg
    21181 tggagtctgc ctgttgttca ggtgccccgc tggcggctac gatgggaacg ttggctgggt
    21241 ccgcaaagcg cacatccact tgataatccg cagcatcata gacaagatgt tcgccgtaat
    21301 accagggttc gtgcaatacc caatcaccat tgatgtaagg cacaacaaac ggataccagt
    21361 tcgtcaggtt caattgccgc cgcgtgtacc cataaatgcg cggacgtgaa acgctggggt
    21421 cttcctgctc ggcaaaaggc aggttgagag taaattgaat attgatgacc atcacctctt
    21481 caggcttgaa aaaaaacggg agcgaaatat caagccgctg tccattgata ttgtaggtgg
    21541 tgatgggcgt gccattgatg gagagaccgt tcaggttgaa actaccctcc cataaatttg
    21601 gcacaatggc aatgaccaat gcattgagct ggttccccgt atgattgggg tagtagatgg
    21661 tttcatccac tgtgacggtg tgggcatcat agtcaatgag cgtatcaagg acatatcgcg
    21721 cgcgttccgg cgcagggaca gtggttgccg tgggaagcgg cgtgttcgat gcagtgggta
    21781 aaggggtgct ggtggaaaaa ggaatggctg tggaggtggg caatgcagtg ggcgacggag
    21841 aaggtgtcga acaggatgaa agcaggaaga tgaaaacaaa aagggtggaa atggatgatt
    21901 gaatattttt tcgcatgggc agtattctac cgcttttgag ctgtgcattg aaataaaaac
    21961 tcccgccgtg gatgcggcgg gagcagatgt ctaccagtaa agtaatgagg aatcagcctt
    22021 cgatcgcttt cttgtatgaa gcaagcgtat tttcaatacc ggtaataagc tgcttcttca
    22081 tcatatcttc catgccgggc atgttgggca ttccaggcgg gagcagtacg gcatccatct
    22141 gaccggtcaa cttggtaccg gagccatccg cttcgagaat ggaggtgttg accaagtccg
    22201 gtgccggggg tgtgccatag gtttttgtgg aaatcagttt atttttctca aatgcgatga
    22261 tttcattcgt gatggtggtt gattttccaa aactggtggt ggtgatcttg tatttggctc
    22321 ccagtttaag cggcccgtcc gactcgatgg cggtgatgta ttcactcatc tttttcatat
    22381 tatcaatgtt ggtgagatgc tcaaacacat cttcgacgga tttgttgatc ttgatggatg
    22441 cttcaataat agtcattgtc aatactcctt gaattggtct ggtggataac aatgaaatta
    22501 ttttatacag acttgatcct tttccacaag aacccaagta ggaatatggc ggtagccgtt
    22561 agtactattg cagagcctga cacgatattg aagtaataac tcaaatataa acccaccaca
    22621 gaagacaggg caccaatcaa tgcagagacg aacatcatcg gcaacaatcg acgggtgagc
    22681 agatatgccg ttgcagcggg tgtgaccagc atcgcagcag ccagactgac gcccgctgtt
    22741 tgaagcgaga ccaccaccgt caacgccagc aggacgagca taaggttgcg aagcaattcc
    22801 acgggcaggc gcaaggtggc agccagaacg gggtcaaagg agatgactaa aaaaggtttg
    22861 aatagcaggg ccaccgtaag caaaatggca aaactcaaac cgccgatcaa ccacaggtct
    22921 gaaacactga ccccaagcac attgccgaac aaaatatgtg aaaggtccac tgtataggtc
    22981 tgcgtggtgc tgatgagtgc cacgcccagt gaaagcgccg cagcaaaaag gatgccaatg
    23041 gcagtatctt ccttgactgc gccgtctttg gtgaacaacc caatgatcaa agccaccgca
    23101 acagcggcaa tccccgcacc gatcagcaag tcacctttaa aaatgtaggc gatcgccaca
    23161 cccggcagaa ttgcgtgtgc cattgcatct cccagaaaag ccatgccgcg caaaacaaca
    23221 taggttccca tcacagcaca caacacaccg acaataacgg aggccagcaa tccgtgctgc
    23281 atgaattcat aggcaagagg ctcaagtaac cagttcattc cgcctcaaca tgatggtgat
    23341 gatgctcttc atctggcggg caacactcat caacaagcat catgccgcct tccatcacca
    23401 gcagggaatt tccaaaggcc tgcgcaaggt tttccttcac aaatacttcc tggggttttc
    23461 caaatgcaat aagacgatga ttcagcaaca ggaccaggtc gaaacgtgaa gcggccagat
    23521 tcaggtcatg ggtcgaaacg atggcggtga cttcccgctc ttgaaggtga tcgagcaggc
    23581 gcaaggtggt ttcctgggtg gtcgcatcca ccccggtgaa aggttcatcc atcaacagga
    23641 tgtgcggctc ctgggctatg gcacgcgcca aaaatgcgcg ttgctgctgc ccgcccgaaa
    23701 gctgaccaat cgaatgctct gcaagatcgg caatgcccat ttgttcaagg cttttatgaa
    23761 ccatgccgcg gtcataggct gaggcacggt tcaaccaccc ggtctgtcca aaacgcccca
    23821 tcatcaccac atcattgacc gtgacaggga agcgccagtc cacttcttca cgctggggga
    23881 catatgcaac acaatcctta tggacaccaa gcgcttcacc gtgaatgaag atttgtccgc
    23941 ttttcagggg cagcaaaccc accaatgctt taaacaaggt ggatttaccc gcaccattcg
    24001 gccccacaac ggcaacacgt gatccgtgtg gaacttgaaa gctaaggtca tgcatgatga
    24061 tcttgtcacc atagccgata ctgatgtttt gaacttcgag acgatgaggt gtatgtgtca
    24121 ttggattttc gattttcgat tcacgattta cggatttcga gtgaaagacg cgtttatttc
    24181 tctcccatct tacttcaaag cgttcacaat ttgaatgaca ttattcttca tcatttcaat
    24241 gtaggttgag gcagggccgc catccgtgag cgactcaagg tacaaaccat ccacaacctt
    24301 cacacctgtt tccgaagcga tctgattcgc aaggtcggca ttttccactt caccgaggaa
    24361 gatggcaggc gcacccgaat tcttcacagc atccaccgcc gccgcgatct cctgtgccga
    24421 tgcactggct tcggaactaa aactcggcaa aatcgaatct acgattgaaa acccatatcg
    24481 ggtcgcgaaa taacccatcg cttcatgatt ggtcaccagc aaacgccggt cggcagggat
    24541 cgtctccacc tgttccgtga taaaagcatc caactctttg agttgcgtaa tgtatgcctc
    24601 tgcattggag gcataggtct ctgcaccagc gggatccgct ttgctcaacc cgtcgcggat
    24661 attctcgaca taggtgatga cgaggttggg atcgagccac atatgcggat ctcccgcttc
    24721 attgccgtga ctgtcgcctg attctgcttc atcagcatgt tcttccattt gaagcggggt
    24781 caggcccgca gttgcctcga tcaccagccg ttcgccgcct gcattttcaa gcaacgactc
    24841 gatgaaatgt tcatactcca cgccattgac gatcaacacg gtgctttttg cgatcttcgc
    24901 cacatcggct ggagcaggct gataagcatg cgggtctgca ccgatgggga gcagggattg
    24961 cacctgtgtg cggtctcccg ccacgttctg ggcaatatct gcgaggaagg aggtagaggc
    25021 gagcacactc aaagtgccgt cactgccagt ggaggccgac ccgcacgagg ttaagaaaag
    25081 agccgtgagg gttgacatca ctataatggt attgaaaatc tttttcattt agagctccta
    25141 ttttttatcc cccgccaatg acgagggatg gattaacgtt ttacttgcag tttgcgcaca
    25201 ggccgaacaa ttgcagccag tgttcctgga tctggtagcc tgtttttctg gcgatggatt
    25261 tcgtcagggt atcgaggtcg tcaccttcaa aaaactccac ctgcccacag ttctgacata
    25321 aaagcagatg ctgatgcccc acacccgctg aaatgaaagc ctggcaacca atgggctgat
    25381 gcacgcgttg gatgagatga agttcctcaa gcttttcgag cgtacgatac acagtgacca
    25441 gacccaaagc acggtatttc ttgcgcgctt gatcgtacac ctcgatgggc gtcagggcat
    25501 gggaattgcc atacaccgta tccaccaccg cgcgccttgc cgatgtaacg cgatacccgt
    25561 tgtcgtgcag ctgttgcagc cagttttcag aggacatgtt caccgtattt cttactgaaa
    25621 atcgttttca ataagtatag tagcaaaaaa tacgggtgtc aacccattgt gaaatgaaaa
    25681 tcgaaactct gcagtgaata aaattcgccg agcctgctta aggccagagc catttggggt
    25741 tataccccgc aatccccaga tccgtcactt tgccggtagc cgcctccacc atctgcaatt
    25801 ttgatgccag cggaaaggcg tcgagatcgt acaagtcata caaaatggac tgcccatcgc
    25861 cagaccagct caatgtgccg tgcagagagt tcggcgagtc tgtcactgcg cgtgcttcgc
    25921 tgccatctcg tctcatcacc cagatttggt cgcctcgtgg aatggaaagg tcgcgccgta
    25981 ccacggcgat ccactctcca tcgggcgacc atgccgcaag gatgttctcg aaaccaaggt
    26041 cgggactgat gttgactgcg gcatcggttt ccagatcata aataaacaac tgtgtgatga
    26101 actgattcga ttgaatgatg acatcacgat ataaaacaga ttttccatcc ggtgcccagg
    26161 ctgcagatgc gcccagtgcg ctggcaacag tgcgggtttc tccgctttca aggttataca
    26221 gttgaatatt ttcagaggtg gcgtaactca accactttcc atccggtgac cagcgcggat
    26281 tcgctcccgg caactgctct tcctgaaaaa gtgatcgagc cgtgccggta tttacatcca
    26341 cccaccacag ggttgccagg ccggtggcat ttgcggcctc gaggctgata tgctcataaa
    26401 gcacacgcga accatccggc gaccagaccg gctgtgagca catcgcttcg gtgcaatcgg
    26461 tcagtttttt attttcaccg ctattcacat ccaccagcca gatttcattt ttcaggtcgc
    26521 ccgtctgtgc aatgtatgcg atacgcgaac catcaggcga aggagcaaaa tccaccgcgc
    26581 tgttggggcc gctggtcaat gcctctggtt gagacccgcg gatatctgaa atgaacaact
    26641 gtttgatgcg gtcctgtcct tcaagttgat agacgatccg cgcatcttcg actccgcccg
    26701 tgcctgcttc acccaaacca agagaatcct tcgcccgtac atatgcgccc caagtgagcg
    26761 caaaacctag cgccaacacc accaccgcag aaacgatcgg catgatcggt gcaaccttat
    26821 cgagaaagct gaccttatcg aacagtctgc ggctcgatac catcaacaag ccgatggcga
    26881 tcaacaccac ggccagaccc agactgaacg aaacgatcaa tgccaatccg aggaaaatac
    26941 gattgatagc cactgccacc aacagaatcg cgatcgcatc cgggcagggg acaagtccgc
    27001 cgctgacgcc gagggcaatc agcgagcgcc aggtgatggc ttcgggcaca tcatgcgagt
    27061 gcagtttgcc atcgccatga tgatgcatat ttgcagaagg tttttgaatg ccggtcaccc
    27121 cggtcacttt ctttggcgcg gtggatgggg tgagcgaaat ttttctggca gccttttcct
    27181 ccaccggggc aggttgtctt gatttccacc agtacacaaa tctctgccaa agcaggtaca
    27241 gaccaagccc aacgatgagc aggccggaaa ggatctccag ccacggaatg atatccgtcg
    27301 gcacgatgta ttttgaagcg gcgagagtga tgatgccgag caaaaaaacg gagccggtat
    27361 gcgtcagcgt gacaactgac ccgaggacca ccgcatgcca gacggtcccg cgtgaaccga
    27421 cgaggtaggc ggcgaccaca gtcttgccgt ggccgggtgt caatgcatgg agcgctccca
    27481 aaatcaggga aattcccagc gcaaaaatat aaaacgaaag cgaaaactcc ttcgcgcgga
    27541 caaggtttag aagccgctca aggttggcgt tctgttcggg ggtcggtgcg gcaggctcgg
    27601 gcgtggcagg aggcgttccg ccctgccatc ctgaaagtga aggcatactg ctatcccatg
    27661 cgtcgagcaa tttcacctgg gctgagtctt gtgtggaagg cggaaaaatc tttaacgcaa
    27721 tctgggcatt tttttgctgg ggcgtttcga acttcatccc gtctgtggtg gtgatgtaat
    27781 accagttaag cgatttctgt tcctcaaagc cgtttcggaa gatcaattga tatgcggcat
    27841 ccgagtcgat ggggctggca gaaaggttga taacgatgaa ttcctgccct gcttgaaaca
    27901 ggtctacgct ggagggaaat ttcacaccgt cgagagtcaa cggcatcttc accccattca
    27961 acagcgatgt gatcttcgaa agatggtcat tggcccatgc aagaccttct tcatcgctga
    28021 ccgttccatt ctgatcggca tctgcattaa accaaatgga ttggatcaac aaaggccccg
    28081 ggcggatctc ccattttatg aaaacgcccc cctgggcgat gttgacataa atatgatgag
    28141 tgtacacatc ggcaggatgt gcaagaacgg ggaccgcctg cgtaaatacg agtaaaagca
    28201 acaacaggga aagggtgagt tttgtttttt tcattttcat ttcagggcag ccaatgcggc
    28261 atgattcctt cagcgatggt tagcggttcg cgaaaaccgt gctgccataa tccgatctcg
    28321 ggtgtaaatt caccacgtaa tttaaattgt tgaaaaacca gcgccgtacc ccacgggtcc
    28381 cagagcggtg agttgtaggt gtaccccggt tggtcggcaa tgatgatgcc atcgagcagg
    28441 ccggggtcaa acagccaaaa cacctgcgca ggttcgcctt cgttgttgcg caaactgacc
    28501 accaccgaat cttcagaggg tgaccacgcc atggagttgt aggcgtaatc acgcgagtcg
    28561 ctgtttccca gcagtgtgga agtatcgttc aaagccaggt cggccaggcg caaacgggta
    28621 accaatccat tttcagaaag atcaatgtca gtgtagagga attttgtgcc atccggcgac
    28681 cacgtgaccg cgccaccggt attggattga aaaaggatct gctcgttgtt cgtaaaattg
    28741 aacaggcgga attgatcggc aaggccatca aaggagatca gtttggatga atcgggagac
    28801 cacgacggct tataccctaa aacctgctgg tcttcgtaca ccgggcctgt ttctccattt
    28861 tgcagatcca acacccagat acgcggagac ccatagggca accccgggcc cacaccggct
    28921 gcttcacgtg tataggcaat gagcacgcca ttcggcgcaa tggctggtgt ggtgcagcgg
    28981 tcacgtccgc aatcgagcat gatggcggca tcgccacctg cacggctcat ccgccacagg
    29041 tcaatacccc cctgtgaatt cgccgatgaa aagacaatgc tctcgccatc ggctgaagca
    29101 tcgaacgcaa tgaccttgat ctcttcaggt gaaatgggct ggggcgaact cccatccagt
    29161 tccaccgtcc acaagatgct ttggtcgttg ttggcaacca gatacacgat cagtgggtca
    29221 cgcacacgca tgtcccaact ttggctgctc tttatttcgc ttccattttg ggtgagtctg
    29281 ccggggctaa gagtcagctt gtaactggca ccccgttcaa acggtcgggt gggaacgaac
    29341 tgcatggtac gcgcatcaag ccattcgatg gtcccatcca ctttgggttg aatcgaaaaa
    29401 agggactcag ccagctcatg atcgacggat tcggaaaagg tcaatttgat cttttggaac
    29461 ggcccgatca gactccgctc gggcagggtt gctgtcaccc gaacaccgat ctgccccgaa
    29521 aaaagaatca ccagaccagc cgccacaacc aataaagcga tcaggccaag cgcaactgtg
    29581 tcaagcgtta tacgtggttt catggaaaga ggtagggttg ttcaggaaca ggaaccgact
    29641 gcaccgactc ggcctggatc agcggcacag ggcgatcatt cagggtgatg atcttgatcg
    29701 gtcccttgac aagtacccac gcatcttttt ccagtgtgga ggcctgcggc cattcgccga
    29761 ccatggcaat ggcaaatcca tcggctgcac agcatgaaac gacaaaacgg ctgacaaaaa
    29821 attgattctc gcccagttct tccgtgaaat acacaaagcc gatcaccgat gctttttgcc
    29881 cgataaattg ctcaaggtca gattgataat tgaagagttt gatccaatcg aggatggtgc
    29941 gttcctctga gtctgtttca aacaactgtg ccgaagagtc ggcgctgacc aacggcgcat
    30001 tggtgttaaa ccctttggtg gcaagcgcag aagaatcgag cgggcgcgca ggaattagca
    30061 caccgatcaa cagcggcagg agcatgaacc acaggttcgc cgagacaggt tcatggtcat
    30121 gatcgtgatc atgatcgccc cctttacgct gacgagagcg tttgatctcg gtgaacaggg
    30181 tctgcgccag aatggcaagc aggatgattc cgaagatcgt cagcgggaca aaacgtagat
    30241 taatgtaata ggttaattgt ccgctggcgg ctttcgatgc aaaaaaaatg cacagaccca
    30301 acaaaagcag agcttgaaaa gagcggtaaa gtgttttagg catggggaat tcctatggct
    30361 gtaagtaatt aaagaggatg cctgccaaca ggctcattaa aaatgggatg gcaacaatgt
    30421 aagccaccga gcggcggcgg aagacctgca aatacatgat gacgctttta atatccacca
    30481 tcgggccaaa gaccaggaac gaaagcaccg aaccgaaact aaatgtgcca aaaaagccaa
    30541 gagccacgaa ggcatccact gtagagcaaa tggaaagcag aatggcgagc gccagcatga
    30601 ccagcacaga ggtgacaggt ccgctgccaa tggcaagcag actggattgc ggaataaagg
    30661 tttgcaaagc cgctgcaagc atggcaccga tgatgagata acgtcccatt tcaaagaact
    30721 cgtcggcagt gaccagcagg gcgcggcgca tcttttcatt gaaagaggcg gatgcatcca
    30781 tggaatggtc atgttcgtgc gccgaggtca acacagggcg caatacagcg gatgcatcct
    30841 gttccaccga aaagaccagc cccaccacga cagcgatgag caaactgact cccatacgcc
    30901 agagcagcat ttgtccccac ccaaaggctg aagccgtgct aagcaccaca atgggattca
    30961 acacaggcgc agccaacagg aaggcaatcc cagcggagag cggcagaccc ttattgaaga
    31021 gtcgccgggt gaggggcaca actccgcact cgcaaacggg gaagaccatg cccatgaggg
    31081 ctccacctac cgcggcggca acagggcggc gcgagatcca cctgctcatt tggtcgcggt
    31141 caagaaagac ttccaccaac cctgaagcaa gggttcccaa cagcaaatag ggtatggctt
    31201 caacaaaaat accaaggaat accgtggcaa agacattaag cctttcccat accacagcaa
    31261 agaatcctgc aggcagcatg ctggcaacaa ccagcacaag aactacaagc gcgatcgtaa
    31321 caatggatgt tctggacaat aatcgtgggg atgttttatt ttgcacaaca ggattatacc
    31381 cactggccgc cggtttgttt tgaatcgcaa aagatttttt tcaccgcata atttcgataa
    31441 ttctgctcgt aaattcacac ttttgaaagc ctgatttaca gcacgtcttt caattccacc
    31501 ataaggtatg attgcgaaaa ccatgttcaa aagattgttt gggcagatcc tcctgcacct
    31561 tggagaaatg gaagaggagt tccgtctctt ccatttacgt gatgacatcg ccacgttcat
    31621 catcacggct gtttgcctcc tgctggccaa tgcgatcatg ctgcgtgtgg atttcatcct
    31681 ataccgctcc agcatggacc tgttttatct gatcatcccc ctccgctcac ttcaagtttt
    31741 tctttcaggc gcctttttat tcctggtgta tcgcaccacc caggtgcaga agttcgatat
    31801 gttcgtcttc acatggctgc ttctcaatgt gatctatttc tccctgttca actttacacg
    31861 cccggcagat tacatggcga ccacctttga tctgttattc atcttcgggt tatatctgct
    31921 atccccactg caattaaaac aaacccttgc cttgagtgtc agtctatccc ttgggtcttt
    31981 atatgtaacc tattattaca aatccggcat accgtatttt attgtggggt caaccacggg
    32041 ggcacatctc ttcatccaca tgctggggct ggcctctgca ttgcagatcc aatcttttaa
    32101 gcgcagggct tttcaggcgt atgcacgcga aagaaaagcc cacgagatca cacaagacct
    32161 tttgcggatt gatgtcctta ccaaggcttt atcacgcaga tattttttcg agcaagccca
    32221 gtgggaattc gagcgttcga aacaactcaa cctgcccctc tcggtattaa tcatagacct
    32281 ggaccatttc aagaagatca atgactcgaa cggccaccat gcaggggatg aggtattgag
    32341 ccgttttggg catttcatcc tccaccaaaa gcggtctcaa gacctgtttg gcaggttggg
    32401 cggcgaggaa ttttccattg tcctgcctga agccaaaacc aaagaggccc aaaacgcggc
    32461 caggcgtttt caagaagcat gggtggatac accggtcgaa gtagactcca aaattattca
    32521 ttcgaccatg agcattggca ttgcggaact ttcccaaagt gaccgtaact tcgacgaact
    32581 actcaaccgt gctgactcga tgatgtacaa ggccaagcaa cgtggacgaa atcgtgtagc
    32641 tcacaaataa catcttcttt tcattcattc ccccgcttga agttccctgt cacacgggcc
    32701 atgcgcaatt gggcattttc atctgtcagg ccttgcaaaa atttctcagc agccccattt
    32761 ttgagggtct tgaccagcct gccatgccag tagatgaaaa ccttcccatc tgcagtttct
    32821 ttgaaagtaa acggattttc ctgtaatttt ccacgcctgt ctattttctt cattgagact
    32881 tttgttaccc caatccttta tgccatttga caacgcgatt acggccatct gccttggctt
    32941 tgtaaagagc tttatcggcg cggtgaaaga gtccttcaaa agtatcctgt tcctgaattc
    33001 cgaactcagt cactcccatg ctgatcgtca ccttgaattt gagggtatca acattgataa
    33061 cggttgaagc aagggcctca cgcagacgct ctgccaggtg aagcgcggtc ttttgcgaag
    33121 tttcaggcag caacagggca aactcctcgc cgcccaatct tgcaaaagta tcttcttttc
    33181 ggatgtgatt tatgcagata ttccgaagcg cgatgagggc atggtccccg ctggcatggc
    33241 cgtaggtgtc attcaccttt ttgaagaaat ccatgtccat gagaaccagg gacaattttc
    33301 ttttataccg aagcgcacgc cgaatttcct tgcgggccag gctcataaaa taaccgcgat
    33361 tggcagcacc cgtcagcgaa tcactgttag ccagcaaatg caattcactt tgaaccttct
    33421 taagcttggt gatatcgtgc aggatgatca agcgtccgat atttctgccc ttgtcatcca
    33481 caatgaacga tgtctgcata tccacgtaac gttttgtctt ccgccttatt tccatttcaa
    33541 tttgcggcag ggtgttcttt tcataatgct gccatttctt aatcacctcc tccaccggct
    33601 tgccaatttt atctgagttg atgttgagga ggttttgtgc agcggggttc atatcgacaa
    33661 tacggttgtg agaatcaatg accagaacac catcacgcat tttttcgatc aaaatatcac
    33721 gcgccacggg gatcaaatca agcaggtgat atcggctcac ggcaaaggca aaagccacag
    33781 cggcaacggt aaaggaaaaa ggcgtattat cggcgccagg cagtggattc aacccaacga
    33841 taaagacgat actgttgagc caggtcacac caattcccac cgcaacaagc cccaattgtt
    33901 ttcggtaaat cccagaggcc cgaataaaac caccgacaat caatgccgta gcgaccaata
    33961 tcaaggtgta ggaataggcc acattcgccc aaaaaacagg cccggcgtcc agaatgaccg
    34021 cactgttttc agcacgtttc cctgcaaaaa agagaccatg atatggatcg gtaaacaggc
    34081 aggtcaatac gaggagcggt tcaagataca gaattaaagt aaaaggccgt ttcagccatt
    34141 cctgccgatt tgtgatctgt aaagcaaagg aaaacaatgc ccccggaacg atcactgcgc
    34201 caacataggt aatatcaagc cagaaatatt cggtcagccc cggagtccct gcccaaaaga
    34261 ggccataggt ggcatcccac caggcaaggg ccagcaacag agcgatcaac gagtttgcac
    34321 ccgccgcatt tttccgcgac cgcgaaacaa tcacagccac gaacaaacag gtaatgctag
    34381 caatgagaag gaatgaactg tacgtaatgc ttggagggga taattgcatg agcaaccttg
    34441 cccctattgt acatctttgc acgttcaaaa aacatgagta gtttattctt ggagataaac
    34501 acagggcagt tgctttaaca caactgccct gcccacattt ctgattaatg gtgatttggt
    34561 tgcagctatt tttcagcctg ccttttcaat gcagagacaa aaccctcaaa gttcgaagtt
    34621 agatcgggaa tattcctgcc aaagagcggc agcaacagac cactgaatat ctcttcggtg
    34681 tgaaaatccg tccctccatc ttttgacgga gtcagtgtgt gggtgcgctc ggatttaaac
    34741 aatcccaacg gcaggccgcc ggtcaatacc atctttttac cgggctcgaa gaccgtcacc
    34801 ttcaccggaa aggattgggc gctcagcttg gtaaaaaatg tcactgtttc gcccagagcc
    34861 agcctgccat caatgtggtc catgcttaaa tcccaggctg ggtaactact tgcatctgaa
    34921 agaatcttcc agattttttc aggcgaggca ttgatcgaaa tggatgcgcg atatgatttc
    34981 atttgtaaag tctcctattt ttcgttcttc ataatctgtt taatgtagtc tttcaacgcg
    35041 gctttattga aatccatcga cgcaatccgc atcgtccgtg ttttactgcc atcaccttcc
    35101 aacaagccgt atttatcatg gagagtggct ccttcaaaaa agatcaaaga aaccctgcct
    35161 ttcccaacca cggtttgaat atggttcttt gttgttccat atacaaggca gttcttccat
    35221 ttgatacttt cattaagaac aggccccgct gccagaacac aagcccgtat ctccaaaaag
    35281 agcggagcga tttcaacatc cagctcgcca atgtactcat tcacaatatc attttttgat
    35341 ttggcgtatt tcacaataat ctcctttgag catttttcat atccccattt tatccacggc
    35401 aggcaaaacc tcattgtacg aagcggacat caggttatcc tgcaagatcg aactgttttg
    35461 aaaatactcc acaggcgcaa gtccggtcat ctttttgaac gaacgattca aatgcggctg
    35521 gtcataaaac acatccggcg tcacatgctc aatgatacgc ggattccgtg ccgaaaaatc
    35581 gcagacctgc cccaggaaca catgaacgtt ttggatgttc tgcaggtctt ttctactcaa
    35641 cccaaacact ttttgataca aacgccgttt atgccttgcc gaaaaattat ccaaccaatc
    35701 cgattctttg aaattcagcg acaatatggg atgtggcaat ttcgggtgct gctgcattcg
    35761 cacatgttcc atcacctgca gtgtaaacgc ttcaaggtcg ttcgtgtctc gcttaaccca
    35821 atcctgcttt aaaaaacaat tggcttttat ctccccggaa aaagactcgg cgaactttag
    35881 cgaaaaacgc gcaccaaaca aaaccagtgg tgtcgaaaaa aacagcttta cagggcgtgt
    35941 gtaaaggttc cgcagtgact gatgaggcag ggctgatttt ctccccgctc cgccatccac
    36001 tatgtgaacc tgtccttttt ggataaaaag ataatcgaag aatggctccg gtataaagtg
    36061 ctccacactc atcaccgcca tgggagactc catcgtcatt ttccagaaaa ataaaattcc
    36121 aagggaggga tctccctttt tcctttgaaa agaaatcttc atcagtgttc caactcatcc
    36181 ccgaattcaa caaatggatg caggcttttc tcttccaact ggtagttggt caccttgcgc
    36241 attccaccgc tccctgtagc atggatcggt gctttggata tttccgccat agtggaccag
    36301 cctgcaagag aaaccacaag cggtgatggt gatgcggtca actggtccat gtccactggc
    36361 gcaagcacac accaaatgac caggtcagct tttttcttta gagtcggata ttgatccaca
    36421 gcaatacagc ggcccaattc agcccagtaa cttgcgctcc aggtctttac ctcaagcctg
    36481 cacacctgcg aataattttt gaccttcaca acgatatcgc acaagttctc acgctccgca
    36541 aaacggaaat gcgaatcgag tttgaatctg ttttcgagca gatatttctc cgttgcaagc
    36601 tcgcccagtt tccccgtcag gtgggaggcg aggcggttgt tgtaatatcc atcgcgttgt
    36661 gaccatttct caatggaggc atgggctgcc gatgcagccc atctcacatc agcggcatcc
    36721 aattcaacct gaagggcttt ttgcgcttcc agatccatcg cagattccaa gcccgggtta
    36781 ttttgtttct tccacgacct gacggatcag gtccacgatc agggcgggtt gttcgagttg
    36841 aacaaaatgc ccgctattct ctacaacaac aagccgcccg cgcgaagaaa agctggcaag
    36901 ataatcctgg gtggtgtccc aaatttcgtc tggtggataa tcgtcggcgg gacgaatgcc
    36961 cgctttgaca acgaccagcg gcaggtcggc gagagaaacg agaccctggg tctggattcc
    37021 gctttgttcc atggcagaat attccccggc gacagcgctc cacatgccat catcgtaata
    37081 ggcggctttg taggcaggct gaagttcttc aggcaataga ccggcttgca acatttcact
    37141 caacgggagg atccccaatt cacccacgag gcgcacctgc cccaatggtg cgatcgcttt
    37201 caaggtctgc gcaagtggta atgactgtgt gatgacatcc ggcacaggga tgaacaggtc
    37261 cggactccat gtttcgatga gcaccatgcc cacaacttca tcgggataat tattttgata
    37321 caacaaggcg gctggtcccc aaaaagaatg ggccacataa acaaatgggc cagtttcacc
    37381 tgagtttctc aacaaggtat gcaattcctt gacgatcacc tctgcagtgc gcggatttgg
    37441 ccccgcctca ctccacccat accctgccct gtcgtaggag cagactcgtg taaattttga
    37501 tatctccggc tgaaccttct gccagtgaat ggaccattca ggaacgccgc cttccaaaat
    37561 gacggttggg ctcccctcgc ctgtgcaata gatatgcatc atatgcccat ctacattggt
    37621 caattgacca agcggcagat tttgtcttcc ttccactctc tgggcaatca cctgccacac
    37681 ccacccgatc ggcaagggga tgatcaacaa gatcagcaat atccttccga tgcgtgaaag
    37741 ccagtaaccc aaattctttc gttgatgcgt tgttgtcata tggtctcctt gcagccaggg
    37801 cacttacaaa aatttaaaca atggaattat atctcccaca tcagactttc attaacctcc
    37861 cccttcgtcc agttgcgaat ttggtcttcc tgtcttatag tccgctggtc tgctggtcaa
    37921 agtgtttact gcccaacaac cagcagaccc aggacaggag actattttta tgcacggata
    37981 cggctggttc atggccatgc gtcagggcga cgaaaagccc aaagtgactc gcgagctttt
    38041 attgcgcgtt ctcaattacg ccaaacccta ctggtggcat attacaggca tgcttgtaac
    38101 gatcctgctt agcacgggtc tatcactgct gacaccgctc atcttccgca acatgatcga
    38161 tgtggtgttg cccgccaaag acacccagag gctcatctgg ctggggctgg cgttgttgtt
    38221 catccccgca ctcaacggag ccatcagcgt cgttcaacgg cggctcaact ccactgtggg
    38281 tgaaggtgtg atctatgacc tgcgcgccag tttgttctcg cgcctgcagc ggatgtcact
    38341 gcgtttcttc accaacacca aaacaggcga actcatgtca cgcctcaaca gcgacgtggt
    38401 cggcgcgcag aatgccatca gcaacaccat cgtcaacatc gtcaccaact tgatcgaagc
    38461 cattgcatta ttcgcagtga tgatctcgct tgaatggcgc ttgacccttg tcagcatttt
    38521 gatcctgccg ctttttatca tcacagccca gcggctggga acagtactgc gcgatatcgc
    38581 ccgacaggca atggatacaa acgcccaaat gaacgcccac atgaacgaga ccctcaatat
    38641 cggcggcgca ctgctcgtta aactgtttgg gcgcacacgg gaagaggaaa aacgattccg
    38701 cgaacgcgct gccaatgtcc gcgatattgg cattcgccgc gctgtgatcg gctcaacctt
    38761 ctttgtcatc tttgggttgg tcaccgcagt tggcacggcg ctggtttacg ggttgggcgg
    38821 ctacttcgtc atcacagatg tcttcaccgt gggcaccatc gtcgcttttg gttcgtacct
    38881 tggtcaactg tacggtaccc tgcaaggcct cgccagtgcc cctgtggatt tttcaacatc
    38941 catggtcagc ttcgaacgcg tctttgaaat cgtggacctt ccgcaggata ttgccgaaaa
    39001 agaaaacgcg attgaactgc aagatgtgca aggcgaactt gaattcaaag acgtcacctt
    39061 caactacaaa gtggacgagt cgatcctgct caaagatgtt aaacgttatg gcagcatgga
    39121 taatgtaaaa gcggtcatca gcggacaacc ttctgtggat gggaaaaact ctcagggagg
    39181 aaacggctct tcgtccagaa tcaagaatcg ggagggaagg aacgccgctt ccgactcacc
    39241 cgagcctgat tccacatccc aagccagaga catcgccctc gaaaatatct ccttcgtcgc
    39301 ccgccctgga cagttgatcg cccttgttgg cccatcgggc gcaggcaaaa ccacggtgac
    39361 ctatctcatt ccacgcctgt acgatcccac agatggcatc atccgcattg acggtcacaa
    39421 cctgaaggat gtgactcttg attcgctggc tcatgccatc ggcatggtaa cgcaggagac
    39481 ctatctcttt cacgatacca tccgaaccaa tctgacctat gccaaacccg aagccaccca
    39541 ggcagagatc gaatccgctg ccaaggcagc caacatccac aatttcatca tggacctgcc
    39601 cgaaggctac gacaccatcg tcggcgaacg cggctaccga ctcagcggcg gcgaaaaaca
    39661 acgcattgcg cttgcgcgtg tcattttgaa aaatccacgc attcttgttt tggatgaagc
    39721 caccagttca ctcgatagcg aatctgaagc gctgatccag gatgcgctta aacgcgtgat
    39781 ggctggcagg accagcattg tcattgcaca tcgtttatcc accatccttg cggcggatct
    39841 catcctggtg atggatcgcg gcaaaatagc cgaacgcggc acacatgaag agttgcttgc
    39901 cgctggcggc atttacagtc aattgtacga aacgcaattc aggggagagc gggtttaaat
    39961 tgctgtggag cattgtcagg ctattaatca gcctgccaat ttctcccaca gttgacgtgt
    40021 gatctgcaaa tgcccaagat gaagtgtgga atgctccaaa gcatggagca gcgcccaggc
    40081 cacggtacat tctccctttg aagatacaca cagtctatca aggtcttgta aagagagtct
    40141 ttcaagggtg gaggcggcgt aatcaaaact gtcatcaagc cgctttttca ggaaatccat
    40201 tcccaaccca cggactttga attccgctgc gcggtcacgg ttcgatgact cgccgactgc
    40261 cacatcaccg atccaatatc gttcggcacc ggtaaggtgg tacaccaaaa cacagatcga
    40321 attcatctcc tgcccggggc cccaatccaa cgcctctgcg ggcaggtcgt tcaacacggc
    40381 atggatatcg ttgtgacatt tttgtaacag gttcaggtaa tcagaaaaaa aggggtgcat
    40441 ctcattgctc cattccaatg tattgataga tttcttccgc ttttgcggtc agccagccat
    40501 ccaattcagg gatctgctcg cttaaccttg cgcctgccac gatcggcaac cagcggatat
    40561 attccgccct gccctgcgga cgaaggcgaa aatattcacg cagatagatt gagtgaaaga
    40621 cccgcactaa aaatttcaac gccgggttgt gcatttccct gttttcgaca atgccaagca
    40681 ggataatact ggtccgcgcc acatcggcaa gtggattacc gcgtgaggtg tccatccaat
    40741 caatgatgac ggggtctgat tctgtcagca acacattgtc aaaatgaaaa tcgccatgac
    40801 agacacagtc actttcgggg agggaatgca aaacggtcaa caaagcagtt ttgagcggag
    40861 tcggaagcgc aggtgcattt tcgatcttgc gctgcaattt tccgcgtagt ttgggcatat
    40921 ccgcaccgaa aacaaaatca tgcatccgag catggagttg ggcaagggtt ttggcgcagg
    40981 caaataactt ccacggttta tgttgaagca tggccgccat cgactcgccg tttacacgat
    41041 cataaaccag cccattgcgg ccattcaccc gaatcaattc atgcgccaca ggagatttta
    41101 tgccgcttgc atgcacggcc cgcgcaatgc gcaattcata ttctatgtct tcaagcggga
    41161 accagtgatg aaacaatttg agaacatagc catcatccca tttgtaaaca tcggcggtac
    41221 gtcctcgggc cagtgggtgg tcaagagaaa tgtgtttcat actcttgcgt tcattatagg
    41281 gtattttgac aacgcttgac aacttactga ctagtaagta aaatacggtc acaatggtca
    41341 aaattgagaa ctccaaaaag acgattctga atctggcgga ggccctgttg caagacaagg
    41401 gtttcaacgg ttttagttat gcacatattg catcggaact tggtgtaaaa aatgccgcca
    41461 ttcattatca cttccctaca aaggaagacc tggggcaggc ggtgatccaa cgctaccgcg
    41521 accgcttcaa attatgggtc aacaattccc gtgtcaaaga ccttgctcca caggaaaagc
    41581 tggattggtt tttcagcatc tacaccgcca tgcgttccga tcagggcaag gtctgtctgg
    41641 ttggttcaat ggaagttgag ttcaactcga tccccgaagg tttgcaggcg gaagttcaag
    41701 ccatgcataa agaattgctg gcctggcttg aaggtacgtt gaccgaaggc aaggcggcag
    41761 gtgtgtttcg cttcaacggt gaaccagcca acaaggccgc attgatcctc tccactttgc
    41821 agggagggtt gcagatggcc cgcgcgctgg gaacaaaaaa gttccgcgat gtcatcgagc
    41881 agcttaaatt ggatctgctc gcttaatttt tttatcatca aaacttactg gttagtaagt
    41941 aaatcacaag gagaatcaat gacaactttg gaatcaaccg caacaaaata tcaggggatc
    42001 gtgaagaagg cgtatcaggc atttttcagt ccatctcaat acgaagtgaa atccgcagac
    42061 cgtgagatca tcgcacgcgg aaacaattac cgcatctctt ttgaaggcgg cgaattggcg
    42121 gtcaccacct ggggcgaaag cggccccgct gttttgctga tgcacggctg gggcggcgcc
    42181 cgcgcgcaaa tgacgggctt tgtcgaacct ttgctggctg cgggatatcg tgtcgtggca
    42241 tacgaccagc cagcacatgg tgaatccgat ggaaagatga cgaacatcct tgagatcacc
    42301 cccaccatgg atctggtcga gaagcgtgaa ggaaaattcg atgcgattat cgcccattcg
    42361 ttcgggactc tgatcacatc ttatgcactg gtccatcgga attttccgcc tcccaccaga
    42421 ctggtgtatc tgggcgcttt caatcggctt ttggattccc ttccgcgttt tcaagtgctt
    42481 gcaaacctgc ccgatgagat catcgaaggt atgcgaaaca tgatctacga taattttgga
    42541 agaggcgaac tggactccat catcaatgag tccatggtgc agcagataca tattcctgca
    42601 ttaatgttcc acgatgcttc ggataacgtc acccccgtgg atgacagccg cgccattgcc
    42661 agtacctgga aatctgcgca attcatcgag acctcagggc tgggacatcg aggtgcgctg
    42721 caatcaaagg aagtccacgc gcagattgtg gattttttac agaaaaagta atctgatttc
    42781 cacccaagaa acaaagccgc aaagtggcgg ggaatttttc ccctgtcact ttgcggcttt
    42841 gcacaagaaa tacaagtaca atcccttcag ccaaatgacc aaaagggatg agcttatgaa
    42901 aactgcgttg atcttcttct ttgccgttgt tagtgaagtg acagccacta cagccctgaa
    42961 attttctgaa ggatttacaa aattcgtccc cagcctgatc gtcgttgtgg gatacggcct
    43021 atccttctat ttgctttcgc tcagcttgaa ggttattccc atcggcacgg catacgcgct
    43081 ttggtctgga atcggcatcg tgttgactgt tattgtcggc attgtgttct ggcgcgaaac
    43141 catggactgg gcacgcggta tcggcatttt tctgatcatg ttgggcattt tggtgatcaa
    43201 tctgttctca aactcaatag ctcattaaag gtctgacgaa gcacattgcc gctgttttat
    43261 gtcaatacca aaatcggaca aggtgggtta taaatgccta tcttcgtgaa aaaggataaa
    43321 tgcatgaact tacgcgacct tctcagaatc accagccgca catttgccat cggtatcgaa
    43381 cgcctgcctc gtgttttatg tgatgccacg acgatcgcct atctgctttt gcgagtctct
    43441 gattatcttg aagataatga agaaatggaa gccgacaaga aggtggagct tttgaatctt
    43501 tgggtgaaca ttctgcggga ggaggaggcg gtcgaaaccc tgaccattca attgaccgaa
    43561 gccaatactg aaaatcccga tgcgatcgtg gcgcaacact caaaagagat cctgcagcat
    43621 ctacggtcat tgccctacga agtacaggag atcatcgttc atcatgtcat taacagcacc
    43681 caaggcatgg cgcgctggac acaacacgga tcacacctga ccgatgaaaa agacctcgac
    43741 gattacatgt tcgaggttgc aggccgtgtc ggctacctgg tgacacagtt attcgcatgg
    43801 tattccatcg ccattcgcat caaggaaaaa gagatcatgc cgctcgcacg cgagtttgga
    43861 ctggggttgc aaacggtcaa tgtgattcgc ggcctgcgcg aagacttcaa ccgcggctgg
    43921 gtctatgtgc cgcaaaagtt cctctccgaa atgggactga caaaagagca attgttcgat
    43981 ccgcaacaca gtgaacaggc gctgaaagta ttggacatga tgaccgacaa ggccgagcgt
    44041 catttgcgaa acgccctgca atatgtcaaa tcactgccat ggtggctgca tggcattcgg
    44101 ctggggtgca tcttcccgct gatgttcgcc atccgcacac tggcggtctg ccgccataac
    44161 actcaggtct tcgaaagcga agccaagatg acccgcaatg aggttaaaaa gatcgtcatg
    44221 gattccactc tgtggggttg gtccaatctc tggctggatc ggtattatca ggaattgaat
    44281 gtggtggggg aatgacccaa accattcagc gttcaatctc gcgcaaaatg gtcgggaatc
    44341 tccttgacat aattcagcgg ctgaattatg attgaggtca ataaagtcta ttggattact
    44401 aggagttata gataaagtga gactttccaa gcgcggcgag tacggccttc gggcaatgat
    44461 cgctctggca gaacccgctg aaaaaggcgg cgccccacag atgatgcaga tcaaagagat
    44521 ctctcttcgt gagcagatct cgcccaaatt ccttgagcag atccttctca cactcaagaa
    44581 tgcagggctg cttcacagca aaatgggcgt cggcggcggg tattacctgg ccaggtcagc
    44641 cagtgagatc acccttgggc agatcttccg cgtattggat ggccccgtgg caccgatcaa
    44701 gtgcgtaagc cagatggcat acgaaccatg cggctgcccc gatgaagaaa cttgcggtct
    44761 gcgcctcgca atgggcgatg tgcgcaatgc catagccgat attttggata acaccagcct
    44821 ggctgatgta accaaacggc aaagcacggt ccgcaaaaag ctcggcatca agtaaagtga
    44881 atgaaactgc gtgcagggga gcgcattttt atttacctca aaagtatact agtactatag
    44941 a
//
LOCUS       DAOQJC010000014        44067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_360_length_44067_cov_9.759134, whole genome shotgun sequence.
ACCESSION   DAOQJC010000014 DAOQJC010000000
VERSION     DAOQJC010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 44067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44067
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_360_length_44067_cov_9.759134"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="PLE39_03465"
     CDS             complement(<1..1031)
                     /locus_tag="PLE39_03465"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HNE67411.1"
                     /translation="MTNSRIPGFYNLTLEERRAKLAEASDRTPEDLLPWATGGLSAES
                     ADHMIENVVGLHSLPLGIGLNFMVNGRDVLVPFAVEEPSVVAGASFMAKLARAGGGYT
                     ATTTEPLMIGQMQLINVVNMNEARLKIYEHKAELLALADAIDPVLKKFGGGARDLEVR
                     TIEESAIGPFLVLHLIYDVRDAMGANAVNTACEKLAPQIEAITGGKVHLRILSNLADR
                     RIARARCTIPVSELTVGFESFKGETVRDGIIAAYAFAASDPYRAATHNKGIMNGVDSV
                     VIATGNDWRAIEAGAHAYAARNGRYTSLSTWGKDKDGNLVGTLEMPMAVGIVGGATKV
                     HPAAQAAVK"
     gene            1120..2805
                     /locus_tag="PLE39_03470"
     CDS             1120..2805
                     /locus_tag="PLE39_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNE67412.1"
                     /translation="MLLTREQLQERLVALHRASLELVKDVSLETLLERIVATACEQVD
                     ARYAALGVLDDEGKLAQFISVGLTDKEIKRIVHPPVGLGLIGALMNTETTIRIPVIHD
                     HPRSVGFPANHPLMNSFLGVPIRIGDRQLGQIYLTEKIDALEFSADDEMIIQMLASYA
                     ATAIKNARLYEQMKQRDLTLTRRNVDMSLLNGIASTLTSSLELDEILNKTLGLVMNYM
                     KVEAGDIFLLEEDKTTLRMVLHRGQAAEAFWTRNIFVVGEGYPGLVAKSRQPMIGRHL
                     ASDTNFLRDAIVQAGFQQIACIPLISGDNLMGVMSVATRANEPLDERNIQMLIAVGAW
                     AGLAIENARLHSNARRLAVLEERDRIGMDLHDGIIQSIYGVGLGLEGAQLMLNEDPQA
                     AKAHIRQAIDGLNQAIRDIRAYILDLRPRQLGNEGLLTGIKRLIAEYRAHTFSEVNFT
                     GPETDLKDLPHTHSLALFHICQEALANAAKHAKAKNVQVAIWTTNERALMEIRDDGKG
                     FDTENMHSSIGHGLANMQTRAHAVGGEFDISSIIDEGTTILVWVPRVIGRVIK"
     gene            3257..4594
                     /gene="dnaA"
                     /locus_tag="PLE39_03475"
     CDS             3257..4594
                     /gene="dnaA"
                     /locus_tag="PLE39_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNE67413.1"
                     /translation="MNAEQAWQSVLAQLQMEMPRASFDTWVRDTRPVAYDNGVITVGV
                     RNAYARDWLESRLASSVNRLLIGILNSNVTVDFVVSQADEAESTPEAESAPATIEITA
                     PEPKTKHVTLNPRYTFDTYVVGSGNRLAHAACQAVAEKPARAYNPLFLYGGVGLGKTH
                     LLHATGNACHASGLNVLYVSSEEFTNDMITAIRTHTTQAFRDKYRSADVLLVDDIQFI
                     AGKESTQEEFFHTFNTLHGQDKQIIVSSDRPPKSLVTLEERLRSRFEWGLSADIQAPD
                     FETRLAILRSKAERTGRQISDEILESIAKQVQSNIRELEGALNRIIAFADLSGSALTP
                     SLVEVALSDLLPNRSDLEPEKIVTQVADYYKLSVEKLMGRDRTKDVAYSRQIAMYLLR
                     EVSKISFPQIGEALGGRDHSTVMSAIEKIKDQYKSGDGRVKKDVDFLTQKLYA"
     gene            complement(4626..5930)
                     /locus_tag="PLE39_03480"
     CDS             complement(4626..5930)
                     /locus_tag="PLE39_03480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNE67414.1"
                     /translation="MKPILKQLLFLFVLNLVFSFAIQGITNSLGLKNNKMAVDHAIWI
                     FTREIGDDSWRPMKMAFDHWKESQGQSLVYSELLLTSKVKFQYPPTALLITRFIENNQ
                     INLLAFSTATTFLFLLLMAASVIATSLYSYGKTDAPKLNAAEKVGFGALLILLLLTFY
                     PAVKAGTLGQIQVWLNAFFALAILCYFTGYETLAGILLGLMASIKPQYALFIFWGLLR
                     GNKRLVIAMIITGAVGLLLGVRVFGLAMYVDYFRGLSFLAQHGESYYSNQSFNGLAGR
                     IFGVKYPEVFNNTKWRGYYFPPYNTWIFAFTQVTSIAILLIGLWKTKSSTTEARSADF
                     LLMGLAATLASPIAWEHHYGILFPIFVCLGLFIWVGESFGKSLWVKIAFVTCYLLAAN
                     VIPFTKFVADSYLNILQSYLFFAAGGAFILLAMVKHQFPYKK"
     gene            6141..7028
                     /gene="rpsB"
                     /locus_tag="PLE39_03485"
     CDS             6141..7028
                     /gene="rpsB"
                     /locus_tag="PLE39_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNE67415.1"
                     /translation="MAVISMKALLESGVHFGHRTNKWDPRMKPYIFTERNGIHIIDLQ
                     QTVKLLNQGYNVIRDTVANGGTVMFVGTKRQAQETVAEEAIRCGMPYVTERWMGGMLT
                     NWSTMYQRIQELERLEKVRDSDEINRLTKKEGLLIEREITRLSTRLSGARSLKRRPDL
                     LFIIDVGREEAAVREANLLKIPIVALVDTNCNPQNVDYVIPSNDDAIRAIKLLVAKVA
                     DAVLEGKAMRKEEEPEQPASADAQSEKPKTRGPRKPVVDVDTEEVGEDSLLGASTLAK
                     LNVSRKGDDVVADDTAAAE"
     gene            7067..7675
                     /gene="tsf"
                     /locus_tag="PLE39_03490"
     CDS             7067..7675
                     /gene="tsf"
                     /locus_tag="PLE39_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNE67416.1"
                     /translation="MEITTQMIKDLRAATSAPMLDCRKALQEANGDFQKAVDWLREKG
                     MATAAKRSDRVASNGIVEMYSHGGGRVGVMVEVNCETDFVARAEQFRSLAHEIALQIA
                     ASAPKYITAEDIPAAELAHEADIARARAIEEGKPEKMLDKIVEGRVEKYKDEVCLLRQ
                     TYIRDESLTIEKLILQNVAAIGESVIVRRFQRWELGESAASN"
     gene            7743..8459
                     /gene="pyrH"
                     /locus_tag="PLE39_03495"
     CDS             7743..8459
                     /gene="pyrH"
                     /locus_tag="PLE39_03495"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNE67417.1"
                     /translation="MAALKYKRILLKLSGEALGGKSGFGIDVDEAESIASRIKEVHEM
                     GVDVAVVIGAGNLWRGKQGLERGMDRSTADYMGMLATVMNAMSLMDALERQNVFTRVM
                     SAIEMRAIAEPYIRRRAVRHLEKRRVVIFGAGTGNPFFSTDTAAALRATEIDADVVIK
                     ATKVDGVYDADPKKNPDAKKFDQLSYIEVLNRRLEVMDSTAITLCMENNLPILVLNLW
                     DTKALLGALHGEKVGTLVSS"
     gene            8642..9211
                     /gene="frr"
                     /locus_tag="PLE39_03500"
     CDS             8642..9211
                     /gene="frr"
                     /locus_tag="PLE39_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNE67418.1"
                     /translation="MSTDEIRDLLKDAETRMSGAIHALTDDLSGIRTGRASPALVERL
                     SVEYYGTPTHLMQLATISVPEPRTLMIKPFDAGSVKDIERAIRASNLGLNPNSDGKVI
                     HLNLPPLDEERRRDLVKQMSHRLEEARVAVRNIRRDLHNDLRDYEKEKLITEDDLKRG
                     EEDLQKLTDKFIEDIARHGGNKEKEIMEV"
     gene            9257..10003
                     /locus_tag="PLE39_03505"
     CDS             9257..10003
                     /locus_tag="PLE39_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNE67419.1"
                     /translation="MAEIPSAIPLEKIPQHVAMIMDGNGRWALQRGLPRLAGHKAGTE
                     NLRRVIRASVEFGVKYLTIYAFSTENWGRPPEEVKGLMYILEDVIDRELNELNKEGVQ
                     LRHIGRLERLAPSLQEKVLDAIEATKNNNRLVLNVAFNYGGRDEIVLAIQKMMKDGIA
                     PEQVTDELVSQYLFTAGVPDPDLIIRTSGELRISNFLIWQAAYAEWYITPTFWPDFDR
                     EEYHRALEAFAHRDRRYGKVSSGELQESNA"
     gene            9996..>10390
                     /locus_tag="PLE39_03510"
     CDS             9996..>10390
                     /locus_tag="PLE39_03510"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNE67420.1"
                     /translation="MRRRLITALGLAVIGVPAILLGGIFYYLLMGTFLIGAAWEYVHM
                     FRAVKFEPQMHLTVGGVALVTLARMFAPEFAMPVFGAAILAALTYHLIAYERGRDQAA
                     LDFCISIGGVVYIGWIGSYLFDLRNLPQGG"
     assembly_gap    10391..10490
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10624..11616
                     /gene="glpX"
                     /locus_tag="PLE39_03515"
     CDS             10624..11616
                     /gene="glpX"
                     /locus_tag="PLE39_03515"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HNE67421.1"
                     /translation="MEVRGATPTRNLALELARVTEAAAMLAGRYMGRGDKEGADQAAV
                     NAMRLVLSTVDMNGVIVIGEGEKDKAPMLFNGEKVGNGSEPDVDVAVDPIDGTRPLAF
                     GRSNSLAMVALAPRGTMFDPGPFLYMNKLAVGPEAKGKIDIEKPISENLKAIAKAKGK
                     QIEDLTTIILDRPRHDDMVAEIRKAGARIRQIPDGDVAAALMTSWPDSGVDVLLGIGG
                     TPEGVITACALRAMGGEIQGKLFARDEDELRRGREAGYDFEKVLTMDDLVSSEDVFFA
                     TTGITDGELLQGVRYYGNHITTDTLVVRGLTGTIRRISATHSTDKLDNLSSVHY"
     gene            11663..11738
                     /locus_tag="PLE39_03520"
     tRNA            11663..11738
                     /locus_tag="PLE39_03520"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11696..11698,aa:Asn,seq:gtt)
     gene            complement(11835..12686)
                     /locus_tag="PLE39_03525"
     CDS             complement(11835..12686)
                     /locus_tag="PLE39_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE67422.1"
                     /translation="MEITTLSQTNWQTQLRALYVIARKDWKVFWRYPLNAVSSIFQPL
                     IWVTPVYFMGKAFSTNGQALGFAGYTGSGDYMSYILIGTALSNFIMTVFWGMGYALKE
                     DMDAGVLESNWLTPISRLHILVGRTLTSLLTTTITSTVMLIIGALLFGFKPTGNIIAA
                     ILTAIPMLIGLYGFGFAFAAIVLVMRDANTMVDVSSFLVQGLTGANFPVKSLPYWLIP
                     ISLMLPLTYGFDAVRGWILKTNTLLPLPVEVALLIGTMFLMLWFGSWFFYRVERNVRS
                     RGTLGQH"
     gene            complement(12696..13565)
                     /locus_tag="PLE39_03530"
     CDS             complement(12696..13565)
                     /locus_tag="PLE39_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE67423.1"
                     /translation="MQTTLSPQSLGRPGFASDLRALWAVVRREWTITMRYPSWIIAMF
                     IWPLIFPMMYILTAHALSGPDGSGLQVFLNTTGATEYIGYIVVGTTVWMWQNIVLWDV
                     GFALRQEQMRGTLESNWLSPTWRFAYLLGHTGPQLVSMLLFVSITAFEFGVFFGVRLN
                     GNPWMIGLMMLAAIPAIYGLGFAFASVVITMKEANAFVFLVRGLVMIFCGITFPVSLL
                     PGWMQGIAKFMPQTYLIHGMRAAAFNNAGIPELAPDLIPILLFGLFWLALGYSLFVWM
                     ERRARTTGAIGQY"
     gene            complement(13567..14625)
                     /locus_tag="PLE39_03535"
     CDS             complement(13567..14625)
                     /locus_tag="PLE39_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE67424.1"
                     /translation="MSDFAIEATQLVKKFPARPGTGDKAVETESAQGSKKKRWQFWKK
                     EPQAMFTAVNGVDLQIRRGEIFGLLGPNGAGKSTTIRMLCTLLEPTSGTAKVNGFDVV
                     AQANDVRRSLGTVLAGERSIYWKLTGRENLEYFAALYHIPAAITKKRVDDLLERMEIK
                     DRANELVEKYSTGMRQRIAIAKALLARPPILLLDEPTLGLDPQAARNLRELIATLKAE
                     GHTILLTTHYMEEADQLSDRIGIIDTGKVIALDTPEGLKRKIDQREVIKLELTGWHDD
                     LSDKLKGISGIETLTTRKQGEADLWEVNMQAQNSRTALPRVVESLSGNGTRLVNMNIV
                     KPSLEDVFIHLTGKALRD"
     gene            14904..15899
                     /locus_tag="PLE39_03540"
     CDS             14904..15899
                     /locus_tag="PLE39_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67425.1"
                     /translation="MKILKERKGLFVKILVSVLFVFLCYGLSEKAFHWAVCGEKERKE
                     LDMLFLMFCEKEADAWFEKRFWEECTKNDKNLPPLTELLISACKEPAVRGFSDGEKLF
                     VIEGKTDEVYFLDLRTGERAPVPDGKQLWDEVFLSPDLVWFDGYLKPGDTGYIPHYVL
                     DLTDGKKYELFDLSWMPLKDGKFDTQNYGYIKSAEHVYFSPKENAVIALAPNFRQSPR
                     QNVIYFDSSLEKLLNDLGVNYELVDYSLADADVPSPTGKYVVRNDGNGIYFSGTNLPI
                     ITGAYSSSCFKSWYYDESAVVIACGISGYLYHNSITHSVSHPIPLPVLKLFLPTP"
     gene            complement(15972..16775)
                     /locus_tag="PLE39_03545"
     CDS             complement(15972..16775)
                     /locus_tag="PLE39_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67426.1"
                     /translation="MKIHPVLTLTLVLILAACNVPSSAPTNAPAEAAPAVEAATATAL
                     EVAAPTETAIPPTPIPPTETFTPTLPPPTASSTSTPTEIPTVDSLKAVVTADTLSCRY
                     GPGAEYLYFYGFRKDFKLTLIGRAHASNWVMVDGLEHKCWVNQKFISVEGDPQTLRVT
                     YPGEYELPESPYYAPPAVLSAENNGGTVTVKWTDVELRAGDEEDADMFIYIIEVWRCE
                     GGQIVFEALASNYPEVTFVDEGGCAEPSHGRVFFQEKHGFAGPAEIPWP"
     gene            complement(16778..17293)
                     /locus_tag="PLE39_03550"
     CDS             complement(16778..17293)
                     /locus_tag="PLE39_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67427.1"
                     /translation="MANSRNKFDTKDSLELGEHAENLFIVLAVRSGWKVSASSKDENI
                     DDHWDYLIEKAGEVFKVEVKSQKRIRRGDKTEQTDFTWVELRNVRGKVGWLFGKADLI
                     AFEKESSFLFVKRLDLLAVVNKKVNLVAKVRTPADAVYKIYIREGRKDKITLLPTSDV
                     EEIKFMEWKKT"
     gene            complement(17295..18089)
                     /locus_tag="PLE39_03555"
     CDS             complement(17295..18089)
                     /locus_tag="PLE39_03555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNE67428.1"
                     /translation="MKAFGIHFQNERFTLVHDDILTTSLVSANSVDLVVTSPPYNVDI
                     QYNSHKDDVSYADYLEFSKKWMSRCFDWLKDDGRFCLNVPLDKNKGGQQSVGADLTTI
                     AKQIGFNYHSTIIWNEGNISRRTAWGSWKSASAPYVIAPVELIIVLYKNNWKKTSGSK
                     ISDIERNEFMDWTNGLWTFNGESKKRIGHPAPFPLELPRRCIKLFSYVDDVVLDPFSG
                     SGTTLLAAVNNNRKGIGIDVDRKYCELARKRILGITPAPVKPKKKK"
     gene            complement(18111..18884)
                     /locus_tag="PLE39_03560"
     CDS             complement(18111..18884)
                     /locus_tag="PLE39_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNE67429.1"
                     /translation="MIEEQPPLENQPTPETQNEPVIVEPQKTNWRVWLAAGGCAVLLC
                     AAVFVGTLIFVGPKFVQQFFPDKVEVAEELPRAVIQANTMGDPHAPIHIIEYGDFQCP
                     YCLRFWNETEPLLIEEYVNTGKVYFEYRSFPFLGPESVLAAEGAYCAGDQGMFWEYHD
                     TLYANWTGENVGDFTQENLIKYAKAVNLDVDAFETCLSEGTHKGTVEQDKAQAEADGV
                     HATPTFLINGHILEGAQPFSVFKQFIEKILEGNFDSQSG"
     gene            19102..20973
                     /locus_tag="PLE39_03565"
     CDS             19102..20973
                     /locus_tag="PLE39_03565"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNE67430.1"
                     /translation="MNATLTSNQNLPKQIVAALVGGFSLFLTVTITWTLGYQLLYAGR
                     IFPGVSVAGVDVSGLSINDAALKLSQTLSFPNTGKILFRAGDKLWVASPAELGMVFDP
                     TASALAAYRYGRSGGLFGALSGQVSARGLGSDVAPVIIFDQRVAYTYLQNIGAQVNQP
                     VVEASLHLEGTNVVAQPGQVGRVLNLDATLIYLGAQLQTFRDGEVPLVIQEAAPKLLD
                     VSSQAEAARRILSQPLTLSLPNYRSGDPGPWTYDVPVVANMIAVIVVENGGGAEMQVG
                     LDPNILRQSLNELKTYVDRQPTNAKFVFNDGTSQLEPIAASNVGRAMDVEASIAAIND
                     ALLRGEHNVSLVVAEQQPLVADTAVGADLGIVQLIAEQTTYFYGSSTERRQNIEAAAS
                     RFHGVLVAPGETFSMGSVMGDVSLENGFAEALIIYGGRTIKGVGGGVCQVSTTLFRTV
                     FFAGFPVVERYSHAYRVSYYEKTSSGSVDSNLAGMDATVYFPLVDFKFQNDTPYWILM
                     ETYMSENSLTWKLYSTSDGRSITWETTGPTNIVPAPPPLLEENPEMKAEQIKQVDYAA
                     QGADVNVTRVVWRNGVIYFTDQFITHYQPWQAICQYGPGTEDPKKLARRNDICVGAS"
     gene            21136..21354
                     /locus_tag="PLE39_03570"
     CDS             21136..21354
                     /locus_tag="PLE39_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67431.1"
                     /translation="MVSSELLEILRCPNCVREKSGTLVLHKDAWLICQDCGRKYPIVD
                     DIPVMLIDEGDKWVNTAQDALPVPPPEK"
     gene            21363..21941
                     /locus_tag="PLE39_03575"
     CDS             21363..21941
                     /locus_tag="PLE39_03575"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE67432.1"
                     /translation="MSEAKMQELIAELLSGDDERAEKTIPALVGFGMAAHSALLDLTR
                     AADVDSRWWGVRALAASPHTRTEDLLPLLSDSAPEVRAATALALCNHPHEAAVEALVQ
                     ALADEDPLTAGLAGTALGKIGAPSVPSLLKVMSEAPVGVKILALRALSEIKDHRAIPV
                     MMKSMGEESALLQYWAQEGLERLGLNMVYVKP"
     gene            21955..>23093
                     /locus_tag="PLE39_03580"
     CDS             21955..>23093
                     /locus_tag="PLE39_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNE67433.1"
                     /translation="MNRFEFLKKIRLPKPKIPSVGRIIFWAISIGLAAATFVFARNFT
                     SCWNITGLPGIAPSSCGTSTNPLAGPDLTAATQEPGAVDTLPTPAPVAPGVELPPAWD
                     GGSRINILFIGLDFRDWQANDGPPRSDTMILFTIDPVTKSAGMLSIPRDLWVNIPEGF
                     GYSRINTAYSSGEGAKLPGGGPGLAMKTVEQLLGVPVHYYAQVDFNTFTELVNRLGCI
                     YVVPEEILVLDPVGPGTDHVVITPGGERKLCGWKVLAYARTRKTEGGDMDRARRQQQV
                     IFALQQKIFDPEVFPSLIAQAPEMYAELSSGIHTNMPLDTAIKLAILGKDITRESIKT
                     GIIGTDMVTFESTTLGGQAASVMKPIMDKVRVLRDEIFTSGGPTSP"
     assembly_gap    23094..23193
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23401..24330
                     /gene="fmt"
                     /locus_tag="PLE39_03585"
     CDS             23401..24330
                     /gene="fmt"
                     /locus_tag="PLE39_03585"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNE67434.1"
                     /translation="MTLRIVFMGSPDFALPSLSTLAAEPNYQVVGVVTQPDRASGRGR
                     ELKPPPVKSLALELNLPVMQPEKLRQPEAMEQLRAWEPDLIVVAAFGQILKKDVLDLP
                     RHGCINVHASLLPRWRGAAPINAAILHGDEETGVTIMQMDVGLDTGPMLAKRSVRIKP
                     DDTAGSVSQALSTLGADLLIETLPAYLAGNLTATPQPEDGFTYAPMLKKTDGLLDFTQ
                     PAIDLERRVRAMNPWPGAWFEWNGNPLKVLRASTVSGEKGLASGSRFTVEGRPAVQTS
                     AGALVLDEIQPAGKKAMPGKSFLAGARDWVAVA"
     gene            24385..25149
                     /locus_tag="PLE39_03590"
     CDS             24385..25149
                     /locus_tag="PLE39_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HNE67435.1"
                     /translation="MFNSKVFLAEFIGTFALVFIGMGAGIVNAGLLGVALAHGFTLAV
                     FAYAYGHISGTHVNPAVTFALALKNVVTWGQAVVYWIAQFAGAILAAFVMKLFLDSLG
                     GPIDAGATTGALTDKAPILAMVMEAILTFFLVNTILHTAVGGKGGRFAGWAIGTTLAL
                     AILGGGPFTGASLNPARTLGPAVFASIPSLTSPNTYLIYLFGPLIGATLAVLVYNFFT
                     SSDEIEEDEEEVVEEVPAVVEEEAPVKKSRSRKSEE"
     assembly_gap    25191..25290
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            25357..26979
                     /locus_tag="PLE39_03595"
     CDS             25357..26979
                     /locus_tag="PLE39_03595"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNE67436.1"
                     /translation="MQDDLLAAKPPQNTSMDDPIAARRTFAIISHPDAGKTTLTEKLL
                     LYGNAIELAGNVRARKNQRSTTSDWMEMERERGISITSTILQFPYQSNIVNLLDTPGH
                     QDFSEDTYRTLSAVDSAVMVIDAAKGIEPQTLKLFEVCRKRGIPIFTFINKMDRPARD
                     PLELLDEIKKVLGMEPVPMNWPIGDGPEFRGVYDRASKGVYLFERTERNERMAPEIFV
                     PLDELGKNNILPQKRYDELVESVALLDEVGVVFDHDLVLQEKQTPVFFGSAVTNFGVR
                     LFLDTFVKYAPQPQPYLSDRGAVVPQDPGFSGFIFKIQANMNPKHRDSVAFLRICSGR
                     FERGMDLVHAQSGKTVRLVRPYKLFASEREIIDEAYPGDVLGIPNNGEFAIGDSLCTG
                     ELVRFAPIPRFQPEHFALLRNTDLGKQKQFQKGLLQLESEGAVQVLFNVNAFKREPIL
                     AVVGQLQFDVVQARLKAEYNVPTELERLPHVMMRWIKGPETAIQNITQRSEVIIARDS
                     REEWAAIFSTPFFLKYFIEKNPDLRFVEITEN"
     gene            27176..28669
                     /gene="glpK"
                     /locus_tag="PLE39_03600"
     CDS             27176..28669
                     /gene="glpK"
                     /locus_tag="PLE39_03600"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNE67437.1"
                     /translation="MSKYVAAIDQGTTSTRFIIFDHGGNVIAVDQKEHRQIYPKPGWV
                     EHDPLEVWERTQEVMKGALQKSGIQAADIAAIGITNQRETAVVWDKSTGKPVYNAIVW
                     QDTRTDVICNELAKVGGQDRLRSKTGLPLATYFSGPKIKWILDHVEGARAKAEKGELL
                     FGNMDTWVIWNLTGVHVTDVTNASRTLLMNLNTLDWDDELLNLLDVPRAMLPEIKSSS
                     EVYGHVTQSGLSALQGIPVAGDLGDQQAALFGQTCFSAGEAKNTYGTGCFMLMNMGEI
                     PVPSKSGLLTTLGYKIGNQKAVYALEGSIAITGALIQWLRDNLGLIQSSAEVESLASS
                     VEDNGGIYFVPAFSGLFAPYWKSDARGAIVGMTRYVTKGHIARAALEATAYQTCEVLD
                     AMEADSGVKLTALKVDGGMVFNELLMQFQSDILNVPVVRPKVAETTALGAAYAAGLAV
                     GFWKDYDELRANWGRDKEWTPQMDTTLRGKLYTGWKKAVTRTFDWVE"
     gene            28776..30482
                     /locus_tag="PLE39_03605"
     CDS             28776..30482
                     /locus_tag="PLE39_03605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNE67438.1"
                     /translation="MNRNEILEQLKANPKISVLIVGAGINGIGTFRELALNGVDVLLV
                     DRGDFCSGASAASSHMAHGGIRYLENGEFRLVREAVQERNRMIQNAPHIVQPLPTTIP
                     IFKYFSGLFNAPLKFLGWLDKPSERGSVIIKLGLIMYDAYTGKTRTVPRHQFFSRAEA
                     LKNWPRLNSEIVNTAKYYDGLISNPERLALEVMLDGEAENPNARAVNYVSMVKAEKDT
                     VILRDELTDQTYEVRPELVINAAGPWIDFVNHSMGLSTRSIGGTKGSHIVIDHPELRA
                     AIGENEFFFENKDGRIVLIFPLQDRVMIGTSDIKIDNPDEAQCTDEEVDYFIGLVARV
                     FPDIKVSREQIVFRFTGVRPLASSGAKTTGQYSRDHHIEVLNGDWTNLGFPIYSLVGG
                     KWTSFRAFSEQVADKALAFLGMPRQKDTRDLAIGGGRGFAVDPDGQKRQIEGFAAWTG
                     LDKERLQTLYLRYGSRTEAVANFMKRAADEPLKSLPSYTRREIMFLAQYEKVARLDDL
                     LLRRTMLAMLGRLTKESVLETADVLGEALGWGSDQKTAEVQRTLRLLRDRHGVVLNEA
                     KS"
     gene            30510..31451
                     /locus_tag="PLE39_03610"
     CDS             30510..31451
                     /locus_tag="PLE39_03610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE67439.1"
                     /translation="MSSNTTVDLVIPVFNEEGVIEHTHKRICAVVDSLAYDFKIIYVD
                     DGSRDGTVASLRKIADSDQRVILLQLSRNFGHQAALTAGMDASQSEIAITLDGDGQHP
                     PEMIPQMLNLISQGYDIVQTQRMDEAQPASFKKWTSGLFYRLINVISGTQVLPGAADF
                     RALTRPALNALKAMPEYHRFLRGMVSWIGFSMVILPYQPDERIGGVSKYSLSKMIRLA
                     MDAVFSFSLVPLYVGLSLGGILLCLAMVEMVYVLSFWVTGQTSNLAPGWSSLMFVILI
                     VGGMLMALLGFIGVYVGYIFQEVKRRPVYLVKGEMKR"
     gene            31448..32092
                     /locus_tag="PLE39_03615"
     CDS             31448..32092
                     /locus_tag="PLE39_03615"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNE67440.1"
                     /translation="MSESRPQPFQITLLRHGESTGNAESRWQGQADFPLNDTGRAQAR
                     ALAERWKSENMKFDFVISSPLGRAKETAEIVASCLDLKVEFDPLWLERDNGEFAGLTA
                     HEVRQNFQHPAFSTPYDPVGVDGEGDWELFLRAGQALHSLLKREPARYLIVSHGGLLN
                     QIMHAVVGLAPQANNAGTRFRFENTAFAQLTYYPHQHRWAIDKLNDHAHWRDAE"
     gene            32153..33253
                     /locus_tag="PLE39_03620"
     CDS             32153..33253
                     /locus_tag="PLE39_03620"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNE67441.1"
                     /translation="MQSSISNVKATDQLKVLVFGAGAIGTYYGGSLALAGHHVVFVEQ
                     PKMVEQLRERGLRLDLTIDERRKTKDAFVVEPRSFVIEPSLEEALKFGPFDVALFALK
                     SFDTVSALEGMKPFADKLPPILCLSNGVENEQAIARTLGADKVIYGTVTTAIGRRGAG
                     DIVLEKLRGTGIAAGHVLSEKLVAAFQNAYLNCRLYPEPHGMKWSKMLTNLIANPTSA
                     ILNMTAAQVFANRKLYKLEIAMLKECLDVMKAQNLEVVDLPGTPVKLLALAAHLPIWL
                     SKPFLSRAAGSGRGGKMPSFHIDLYAGRGQSEIEYLHGAVVREGKKHGVPTPVNEVLT
                     TTLLALTNKEIPLEEFAGQPEKLLANVLTLKR"
     gene            33275..34966
                     /locus_tag="PLE39_03625"
     CDS             33275..34966
                     /locus_tag="PLE39_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNE67442.1"
                     /translation="MIHLTSPNPNVELHLSDGRILSGPRGTQVGEFLRAVKDDFSAPI
                     VAAILNNEIHELTYPIERESDCAPVTMDRADGARIYRRSLIFLLEIAFAELFPKGKLT
                     IDHSVSSGGFYCQVTEREALTQKELDALKTHMEKIVAEDQAFERKQIPIQEAIQYFEK
                     LGYSDKVRLFSHRQKDYLTLYSLNGRMDYLHGYMVPSTGYLRWFDLNCIDGGFTLQFP
                     RRHAPTHLEPMGDYPKLLNTFRQYGDWLTRLAIDNVGALNDAIVAGRADELIMVSEAL
                     HEQHVADIAQQIAQKNSRIILIAGPSSSGKTTTSRRLAIQLLARGISPYPLELDNYFI
                     DRSKTPLDEEGKPDFEALEALDLNRLAQDIEKLLRGEKVQLPRYNFKSGLSEMGDVIQ
                     LKDGQPLILEGIHGMNPRLIPDRWSGAAFRVYVSALTQLNLDRHNRVSTTDTRLIRRM
                     VRDARERGYSATQTISRWESVRRGEKRHIFPYQENADVMFNSALVYELAALKPLAEPL
                     LRQVQHRSPEYIEARRLLAFLEWFLPLDVNLIPANSIVREFLGGSSLKDFKIWRG"
     gene            complement(35156..35788)
                     /locus_tag="PLE39_03630"
     CDS             complement(35156..35788)
                     /locus_tag="PLE39_03630"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNE67443.1"
                     /translation="MRPKRIILIRHAESEGNLDHTRYQTVQDFALRLSSTGVQQARLA
                     GVQLKEILEDGKVYVYLSPFFRTRETFQLIREAISQNIVKSIEDPRIREQDWGHLRHP
                     DDNKGIIEERDNFSTFYYRIPDGESGADVYDRVSSFLDTLHRDFEKENFPENVLIVSH
                     GLTMRLFLMRWFHWSVEEFESLHNPKNCQIVILEKQADNHYILSSELSRR"
     gene            complement(35810..36802)
                     /gene="mgrA"
                     /locus_tag="PLE39_03635"
     CDS             complement(35810..36802)
                     /gene="mgrA"
                     /locus_tag="PLE39_03635"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HNE67444.1"
                     /translation="MNYLPNESRYQTMRYNRSGRSGLKLPAVSLGLWHNFGGVDTFSN
                     SRAMVLRAFDLGITHLDLANNYGPPPGSAEETFGQIIAKDLRPFRDELIISSKAGYTM
                     WDGPYGDWGSRKYLVSSLDQSLKRMGLEYVDIFYHHRPDPETPLEETMQALDYIVRSG
                     RALYVGISNYPAEKARAAAKMLRELGTPCLIHQPAYSMFNRWVEDGLLATLKEEGVGC
                     IAFSPLAQGLLTNKYLGGGIPEGSRASKAHGFLKPANITDEKLNKVRKLNELAQSREQ
                     TLAQMALAWVLRHDSMTSVLIGASKVEQIDDAIGTLKHLEFTAAELEQIETILK"
     gene            complement(36920..38362)
                     /locus_tag="PLE39_03640"
     CDS             complement(36920..38362)
                     /locus_tag="PLE39_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67445.1"
                     /translation="MQKKLTTLIVILGNITVLSGLALLAYLGFYNRYWADDWCYSADA
                     RQLGTIPATLQYFDTEDTGYSSNRYSLTFFSALTENTLGMFGNQLVATLTISLWLVGL
                     VWTGWNVSKLIKPLPFAAILFASSLLLFYNLYLSPQRFQILYWRSGVLPYSTAIVFWM
                     LILGFVTSQINRETPARWVNYIVAPLAFIAAGLGEISCVFLFSGTTLFLLAAWYAKKQ
                     KQHWAEQSFQTLFIAWLFLLLGMIALIISPSNARVANMNVKRSSLFSVPFVSLKHAID
                     FIVFSLKDLPLPHAVFIGIFLGFGIITTKADDSTLTVKRAAVLLAFTTLITYLLITAI
                     QAPSAYFYSATPDPRGQSLSRFTLLLGIAVMAWIAGMWAAQNIKGQWLVLASVVVMLA
                     GYAYTARSIVNIYGERYGFIYRAQIWDERDALIESEKAKGNLLIEVPAIDTGEIHTRD
                     MFGSNGKGWRQFSLNCGARYYGVDGLKVKQ"
     gene            complement(38505..39533)
                     /locus_tag="PLE39_03645"
     CDS             complement(38505..39533)
                     /locus_tag="PLE39_03645"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HNE67446.1"
                     /translation="MTPSSPILVTGASGFVAIHTVIQLLQQGYNVRGTIRSLSKEADL
                     RKTISNYVQANDRLEILSADLSQDAGWDEAMQNVETVLHVASPFPLYEPKHEDELIVP
                     AVQGTLRVLRAAHKAGVKRMVQVSSVAAISSGHNGENKTFSEADWSDLTKDIGAYAKS
                     KTLAERAAWDFIHGSENTNKMQLTTINPPLILGPVPNKDFRTSIELVRTIMLGQVPGV
                     GRIKMGSVDVRDVASALILAMQTPEAAGQRILVAADRSLWLKEIAELLHGKYAGRYKI
                     NKLEFPSILVRLIALFDKKVARVTESLDWDHELSNENAKRILKWTPRSAEEGILSMAE
                     SLIEQGVI"
     gene            complement(39594..39821)
                     /locus_tag="PLE39_03650"
     CDS             complement(39594..39821)
                     /locus_tag="PLE39_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67447.1"
                     /translation="MVIYSSSEIQKQFPVFLKKAMQEGQLRFKTKDGQIFVISPVIPA
                     RESPFEVRSIKLNITRSDILEAVRESRTRYS"
     gene            complement(39824..40216)
                     /locus_tag="PLE39_03655"
     CDS             complement(39824..40216)
                     /locus_tag="PLE39_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE67448.1"
                     /translation="MKIAVDTSAIIAVIADEPEKAKLVELTKGSTIIVPPSVTWEVGN
                     AFSAMMKKTRVNLEQAIEAIKIYQEISMETVEINLQAAIQLAGKHNIYAYDAYILQCA
                     LEHSVPLITLDKDLAETAKKEGVQVIEV"
     gene            complement(40267..41136)
                     /locus_tag="PLE39_03660"
     CDS             complement(40267..41136)
                     /locus_tag="PLE39_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNE67449.1"
                     /translation="MNKPGQLRSYSFILATLRYRKLIAFLTRVMIALRRKKVILIFGM
                     SRSGTSMLGKFLSLGKSSIYMHEPETELMKQHFGPDRHYTQQNFWDFVHSEVPTELKV
                     HTLACISLFAVLKAPIFSKAIVIKPISFVEVIGESSEALTNANIVYICRHPAGRSESI
                     LRQLQHDQGIEEISLEALEQISQDWGRTNKTIKELHRQNPKWLWVYFEKLTGDPVAEF
                     KKLYTQLGLPWNQSIADEIEKSTTGMDGGYYNIQRDSRKQADKWRNSLTTEQIESVRK
                     GTLPFETDLYESF"
     gene            complement(41142..41930)
                     /locus_tag="PLE39_03665"
     CDS             complement(41142..41930)
                     /locus_tag="PLE39_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HNE67450.1"
                     /translation="MQLLLGFLLAILIAYLAYKARSLNQSGAIAAAFTGTIIFGIGGW
                     EWAVLLLTFFITSSALSRAFKKRKQGLDEKFSKGHERDAGQVFGNGGVATFFAALHFF
                     FPESSLPWVGFAAALAAVNADTWATELGVLNPNPPRMITDLRKIVEKGTSGGISLIGT
                     LASLTGSALIAFLASLLTDNWFLFPLITLSGLAGSLFDSFLGGTVQAMYYCPTDKKET
                     EKHPLHTCGTPTVHTRGWQWLDNDRVNFACGAAGTLVALLLFFI"
     gene            complement(41978..42886)
                     /locus_tag="PLE39_03670"
     CDS             complement(41978..42886)
                     /locus_tag="PLE39_03670"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNE67451.1"
                     /translation="MNIGHFPPPKRRGLIVHGSIIIVLMVIAFIGFINLSRAEVGPQF
                     LVALLVSLFAFAPIPFFIYRGYSLWRADYHMDRDSLAINWGLRVEDIPLSDIEWMRPA
                     DDLTRPLNLPSVRLPGGLLGMRRHPDLGVVEFLASDAKKLLLVATAKRVFVISPEDPA
                     LLMQTFARATELGSLAPVEAKSVYPSFVVTQAWESGLARYLWLTALFLNLGLFIWASL
                     IIPSTPRVALGPQFVGSALETVPSAQLIIFPVASLLLAVMGWVAGLYFYRWDRERILA
                     FIVWGSSTFSSLLFLLAVLFIITTPV"
     gene            complement(42916..44064)
                     /locus_tag="PLE39_03675"
     CDS             complement(42916..44064)
                     /locus_tag="PLE39_03675"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNE67452.1"
                     /translation="MPMSSPDLTDADRQAVIEVINTPNLSMGKWTPAFEKTFCDLTGA
                     KHAISVNSGTAGLHLCVRAAGIGAGDLVITTPFSFVASSNALLFENAIPVFVDIDPKT
                     GNINPELVADAAKNIEKFLPRKGADKSGKLKAILPVDVFGQPADMDAINAVAKEHGLK
                     VIEDSCEALGATYKGKQAGTLGDFGVFAFYPNKQITTGEGGVIITSDDQSAHFMRALR
                     NQGRAPGDTWLQHTHLGYNYRIDEMSAAMGASQMTRLDEILTKRNQVAAWYEARLSEI
                     PGVETPYIESFTTRMSWFVYVIRFDKRINRDEMAKKLDARGIPVRPYFLPIHLQPYMV
                     EKFGYKEGDFPVTEDLGQRGLAIPFSGVMTEEQVDFVSNIIREEVMKA"
BASE COUNT        10343 a        10876 c        11810 g        10738 t
ORIGIN      
        1 aatttaactg cggcctgagc agcagggtgg accttggtcg ccccgccaac gatccctact
       61 gccattggca tttcaagcgt gcctacgagg tttccatctt tatctttgcc ccaagtggag
      121 agggaagtgt aacgtccgtt gcgggccgcg tacgcgtgag cgcctgcttc aatggcgcgc
      181 caatcgttgc ccgtagcgat gactaccgag tctacgccgt tcataattcc cttgttgtgg
      241 gttgcggcac ggtatggatc ggatgcggcg aaggcatatg cggcgataat gccgtcacgt
      301 acggtctcac ctttgaacga ttcaaacccg accgtgagtt cgctcacagg aatggtacag
      361 cgtgcgcgtg caattctgcg gtctgccagg ttggacaaaa tacgcaagtg gacttttccg
      421 cctgtgatgg cttcgatctg tggagcgagc ttttcgcagg ctgtattcac tgcatttgcg
      481 cccatggcat cgcgcacatc ataaatgagg tgcaggacga ggaatggccc aatggccgat
      541 tcttcgatgg tccgcacttc gaggtcgcgg gctccgccgc cgaatttctt caacacaggg
      601 tcaatggcat cggccagggc gagtaattcg gctttgtgtt cgtaaatttt taatcgcgcc
      661 tcgttcatgt tgaccacatt gatgagctgc atctgtccga tcatcaatgg ttcggtggtg
      721 gtggccgtgt atccaccgcc ggcgcgggcg agtttggcca tgaatgatgc cccggccaca
      781 accgagggtt cctccacggc aaacggaacc agaacatcgc gtccatttac cataaagttg
      841 aggccgatgc cgaggggcag ggaatgcaag cccaccacgt tctcaatcat gtggtcggct
      901 gattcagcgg agagtccgcc tgttgcccac gggagcaggt cttcgggagt ccgatccgag
      961 gcttccgcta gtttggcgcg gcgttcttcg agggtcaggt tgtaaaatcc ggggatgcgc
     1021 gaattggtca tggttgattg tcccaagtat aggtttgacg ttctttcaaa ttctatcaaa
     1081 aagcgataga ttgattataa tgtctgggag gtatctttta tgctattaac ccgtgaacag
     1141 ttgcaggagc gtttggtcgc gcttcacaga gccagccttg aactggtcaa ggatgtttcg
     1201 cttgaaactc tgctcgaacg gatcgtggcc acggcttgcg aacaggttga tgcgcgttat
     1261 gcagctttgg gcgttttgga tgatgagggg aaacttgccc agtttatttc agtcggcctg
     1321 acagataaag agatcaaacg tatcgtgcat cctcccgttg ggcttgggct gatcggtgcg
     1381 ttgatgaata ctgaaaccac catccgcatt ccggtcatcc atgaccaccc gcggtcagtc
     1441 ggattccccg caaatcaccc gctcatgaac tcatttttag gggttcccat ccgcatcggt
     1501 gacaggcagt tggggcaaat ttatctcact gaaaaaatag atgcactgga attcagcgcc
     1561 gacgatgaga tgatcattca aatgctggcc tcgtatgccg cgactgccat caaaaacgcg
     1621 cgattatacg agcagatgaa acaacgcgac ctgaccctga cgcgccgcaa tgtggatatg
     1681 tctctgctga atggcatcgc atccacctta acctcaagcc tggaactcga cgagattctg
     1741 aacaagaccc ttgggcttgt gatgaactac atgaaagtgg aagcgggtga tattttcctg
     1801 ctggaagaag ataaaaccac cctgcgtatg gttctgcacc gtgggcaagc cgccgaagcc
     1861 ttttggacgc gtaacatctt cgtagtgggt gagggttacc ctggccttgt tgccaaaagc
     1921 cgccaaccca tgatcggcag gcaccttgca agtgatacca actttttgcg agatgcaatc
     1981 gttcaggcag gttttcaaca aattgcgtgt attccgttga tttcaggtga taacctcatg
     2041 ggagttatga gtgttgccac ccgtgccaat gagcctttgg atgaaagaaa cattcaaatg
     2101 ctgatcgcag tgggagcttg ggcgggtctt gcgatagaaa acgcccgttt acattccaat
     2161 gcgcgccgtt tggccgtgct ggaagaacgt gaccgcatcg gcatggattt gcacgacggc
     2221 attattcagt caatttatgg cgtgggattg gggttggaag gcgcccaatt gatgctaaat
     2281 gaggatccac aggctgcaaa agcccatatt cgtcaggcta ttgatggatt aaatcaggca
     2341 attcgtgata tccgcgctta tattcttgat ttgagaccac gtcagcttgg gaatgaaggc
     2401 ttattaacgg gaatcaaacg cctgatcgca gaatatcgtg ctcacacgtt ctctgaagtg
     2461 aacttcaccg gccccgaaac agacttaaaa gacctgcctc acactcattc actggcattg
     2521 tttcatatat gtcaggaggc tttggccaat gcagccaaac atgccaaggc aaaaaatgta
     2581 caagtagcaa tatggacaac caatgaacgc gccctgatgg agatcagaga tgatggtaaa
     2641 ggctttgata cagagaatat gcacagttcc attgggcatg gtctcgccaa catgcagact
     2701 cgcgcacatg ctgtaggcgg tgaattcgat atatcatcaa taattgacga gggaaccacc
     2761 attcttgtat gggttccacg tgttattgga cgcgttatca aatgaatcaa atcatcttgc
     2821 aattgaatcc cggaaatttg agtctcgttt aatatgtttt aaaaacgttt cttctttcga
     2881 ttttttaaga tagatatatg tcaggaacca ttttatagcc catagcaccc atatatgggg
     2941 ggtgggaagc cccaaaacgg tgtgtttggc tgaaacgcct gttctatctc aaatcataat
     3001 cgtgtttgaa tgcaagatat ttcataaacg catgtcaacc ttaaaaaaac aaatcttttt
     3061 aacttaacgt aggtgtaacg ccttgttatc taaagaaagt atctgctaga atgtaggcac
     3121 gctgataaaa tggattgatg cccattgcat ccagatccct gaaaaaaatt tacaaactaa
     3181 tatggtttcc ccaaataagc cgcagaacaa agcgggctgg atttctgtac gataaagtca
     3241 ttatcaagga atggttatga acgctgagca ggcctggcaa tcagtactcg cacaacttca
     3301 aatggaaatg ccccgcgcat cttttgatac ctgggtgcgc gatacccgtc cggttgcata
     3361 cgacaacggt gttattactg ttggcgtacg caatgcctat gcccgtgact ggctggaaag
     3421 tcgccttgcg agtagtgtca atcgcctgtt gatcggtatt ttgaattcga atgtgaccgt
     3481 ggattttgtt gtttctcaag ctgatgaagc cgaatccacc ccggaagccg aatctgctcc
     3541 tgccacgatc gaaattaccg cccctgaacc aaaaacaaag catgtcaccc tgaatccacg
     3601 ctatacattt gacacttatg tcgttggttc gggtaaccgc ctcgcccatg cggcttgtca
     3661 ggcggtggcc gagaagcccg ctcgtgccta caatccgctt tttctgtatg gcggtgtagg
     3721 gctgggaaaa acccacctgt tacatgccac tggtaatgcc tgccatgcca gcggattaaa
     3781 tgttttatac gtttcctctg aagaattcac gaatgacatg atcaccgcca ttcgtaccca
     3841 caccacacaa gccttccgtg ataaatatcg ctctgccgac gtattgcttg tggatgatat
     3901 tcagttcatt gccggcaaag aatccacgca ggaagagttc ttccacacct tcaacaccct
     3961 gcatggacag gataaacaga tcatcgtatc gtctgaccga cctcccaaat cgctcgtcac
     4021 tttggaagaa cgtctgcgat cccgcttcga gtggggatta agtgccgata ttcaagcgcc
     4081 tgatttcgaa acccgcctcg ccattttgcg ctccaaagcc gagcgcacag gccgccagat
     4141 ctcagatgaa attctcgaaa gtattgccaa acaagtgcag tccaacattc gcgaattgga
     4201 aggcgcactg aaccgtatta tcgcatttgc tgatttgagc ggttcggccc tgacccccag
     4261 ccttgtggaa gttgcccttt cagatctttt gcccaaccgc agcgaccttg aacctgaaaa
     4321 gatcgtcaca caggttgctg attattacaa actgtctgtg gaaaagctca tgggacgtga
     4381 ccgtaccaaa gacgtcgcct actcccgcca gatcgccatg tacctgttgc gtgaggtttc
     4441 caagatctca ttcccgcaaa ttggcgaagc gctcggcggc cgcgaccatt caacggtcat
     4501 gtctgccatt gagaagataa aagaccaata taagagcggt gatggcaggg taaagaaaga
     4561 tgtggatttc ctgacgcaga agttatacgc ctgaaattga ttgtagagcg agatgaaatc
     4621 tcgctctatt ttttatatgg gaactggtgt ttgaccatag ccagcaagat aaacgccccg
     4681 ccagcggcaa agaacaaata cgattgtaga atgttgaggt aggaatccgc aacgaatttg
     4741 gtgaatggaa tgacatttgc cgccagcagg tagcaagtta caaacgcaat ttttacccaa
     4801 aggcttttcc cgaaactttc tccaacccaa atgaacaacc ccaaacatac gaaaatgggg
     4861 aagaggatgc cgtaatggtg ctcccaggca atgggagatg ccagcgtagc cgccagaccc
     4921 atcaggagga aatctgcgct tcgcgcctcc gtggtgctgc tctttgtttt ccataaaccg
     4981 atcagcagga tggcaatgga tgtgacctgg gtaaacgcaa agatccatgt gttgtaaggt
     5041 gggaagtaat atccgcgcca tttggtattg ttgaacactt cagggtattt caccccgaaa
     5101 attctgcccg ccaatccatt gaaagattgg ttggagtagt aggattcacc atgctgagca
     5161 aggaaactca gcccgcggaa atagtccacg tacatggcga ggccgaaaac acggaccccg
     5221 agcagaagcc ccacagcccc tgtgatgatc atggcaatca ccaatcgttt gttgccacgc
     5281 agcagacccc agaaaatgaa cagcgcatac tgcggcttga tcgatgccat caatcccagc
     5341 agaatacctg ccagggtctc atatcctgtg aaatagcaca ggatggccag cgcaaaaaag
     5401 gcattcaacc atacctgtat ttgtccgagt gtgcctgctt tgacggcagg gtaaaaagta
     5461 agcagcagta atatgagcaa agccccgaaa ccaacctttt cggccgcgtt gagttttggt
     5521 gcgtcggttt ttccgtatga gtaaaggctg gttgcgatga cactggccgc cattagtaga
     5581 aggaacagga aggtggtggc agttgaaaag gcgagcaggt tgatctggtt gttctcgata
     5641 aaacgggtga tcaacagcgc ggtgggtggg tattgaaatt taaccttgct ggtcagcagt
     5701 aactcgctgt aaaccagact ttgtccctga gattctttcc aatggtcaaa ggccattttc
     5761 atcggtcgcc aggaatcgtc cccgatttcg cgggtgaaga tccagatggc gtgatccact
     5821 gccattttgt tgtttttcaa accaagggaa tttgtgatcc cctgaattgc aaaggaaaaa
     5881 acaaggttga ggacaaagag aaaaaggagt tgtttgagga tgggcttcat ggttttccgc
     5941 gtaatttttt agcctagtat agcattttcc tatggtataa tcgcgccgcc ctgaaatctt
     6001 cagggcaaca ttctgcctgg gacgggcaaa agcgtccctg agcgggtctg gctgatcagg
     6061 ccgtatcccc gctaaactca ttctgttcca tgccagattg catctgcgca cagggaacag
     6121 cacaagttaa ggagtaacaa atggctgtca tttccatgaa agccctgctt gagagcggcg
     6181 tccactttgg gcatcgcacc aacaagtggg acccccgtat gaaaccgtac atcttcaccg
     6241 agcgtaacgg tattcatatc attgacctgc aacagactgt caagctgttg aaccagggtt
     6301 acaacgttat tcgcgatacc gttgcgaacg gcggtacggt gatgtttgtc ggcaccaagc
     6361 gccaggcgca ggaaactgtg gctgaagaag ccatccgctg cggtatgccc tatgtgaccg
     6421 aacgttggat gggcggtatg ctcaccaact ggagcaccat gtaccagcgc attcaggaat
     6481 tggagcgcct tgagaaggtg cgcgattctg atgaaatcaa ccgcctgacc aagaaggaag
     6541 gtttgttgat cgagcgcgag atcacccgcc tttccacccg tttatctggt gcccgctctt
     6601 tgaagcgccg ccctgacctg ctcttcatca ttgatgtcgg tcgtgaagaa gctgccgtac
     6661 gtgaagccaa cctgttgaag atcccgatcg ttgccttggt ggataccaac tgcaatccgc
     6721 agaatgtgga ttatgtcatc ccgtccaatg acgatgccat ccgcgccatc aaactgctgg
     6781 ttgccaaagt tgctgatgct gtgctcgaag gcaaggccat gcgcaaggaa gaagagcccg
     6841 aacagcctgc ttccgctgac gcccagtctg agaagcccaa gacccgtggt ccacgcaagc
     6901 ctgtggttga cgtagatacc gaagaagtag gtgaggattc cctgcttggt gcctccacac
     6961 tggcgaagtt gaatgtttcc cgcaagggtg atgatgttgt cgctgatgat acggccgcgg
     7021 ctgaataatc aagcgcaaga aaaaaagaaa gtctaaggat agtgaaatgg aaataacaac
     7081 acagatgatc aaggacctgc gtgctgccac cagcgccccc atgctggatt gccgcaaagc
     7141 ccttcaggaa gcaaacggtg attttcaaaa ggcggtggat tggctgcgcg aaaaaggcat
     7201 ggctactgct gccaagcgtt ccgaccgcgt cgcctcgaat ggcattgttg aaatgtattc
     7261 ccacggcggt ggtcgtgttg gtgtgatggt ggaagtgaac tgcgaaaccg atttcgtcgc
     7321 ccgcgccgaa cagttccgca gcctcgccca tgaaatcgcc ctgcaaatcg ctgcaagcgc
     7381 cccgaaatac atcactgctg aagatatccc tgccgctgaa cttgcgcatg aagccgacat
     7441 tgcccgcgcc cgtgccattg aagaaggcaa gcccgagaag atgctcgaca agatcgtgga
     7501 aggccgcgtg gaaaaataca aagatgaagt ttgtcttctg cgccagacct acatccgcga
     7561 tgagtctctc accattgaaa agcttatttt gcagaacgtc gctgccattg gtgaaagtgt
     7621 gatcgtacgc cgcttccagc gttgggagct gggtgaaagc gcagcaagta attagtttta
     7681 aaaagccaac cttcgggttg gcttttttta taatttataa ctgaaaatac acggaggtag
     7741 aaatggcagc attgaaatac aaacgcatcc tgctcaagtt gagcggggag gcactcggcg
     7801 gaaaatcagg ttttggtatt gatgtggatg aagcagaatc gatcgccagt cgaatcaagg
     7861 aagttcacga gatgggggtg gatgtggcgg ttgtgatcgg tgctggaaac ctttggcgtg
     7921 gcaaacaagg cctggagcgc ggcatggatc gttccacagc cgattacatg ggcatgcttg
     7981 ccactgtgat gaatgccatg tcgctaatgg atgccctgga gcggcagaat gtatttacac
     8041 gggtgatgtc tgccattgaa atgcgcgcca ttgccgagcc atacatccgc cgccgtgcag
     8101 tacgtcacct tgagaagaga cgcgtggtca ttttcggcgc cgggacgggg aacccgttct
     8161 tctccacaga tactgccgcc gccctgcgtg caaccgaaat agacgcagat gtggttatca
     8221 aagccactaa agtggatggc gtgtacgatg cggaccccaa gaagaatcca gatgcgaaaa
     8281 aatttgacca gctaagttac atcgaagtgc tgaatcgccg tcttgaagtc atggatagca
     8341 ctgccattac cttgtgcatg gaaaataacc tgcccattct tgtgttgaac ctgtgggata
     8401 ccaaggcatt actcggcgca ttgcacggtg aaaaagtagg gacactggtc agttcctaaa
     8461 gatcttgctg tttcatgaaa tgaaatccgc atttcctcct tccattgcga aggaggaaat
     8521 tttttaaata catgagagcc tctcaaattt ttgcatttcc tatcgccctt ttatcactcc
     8581 catagcataa atcgttgttt taaggtatcc ttgtagcgta attattttca ggaggaagat
     8641 agtgagcacc gacgaaatta gagacctgtt gaaagatgct gaaacccgta tgagcggagc
     8701 aatccacgcc cttaccgatg acctttctgg aattcgcacc gggcgtgcaa gccctgcact
     8761 ggtagaaaga ctttctgtgg aatattacgg aacccccacg catcttatgc agcttgcaac
     8821 gatcagcgtt cctgaaccgc gtactttgat gatcaagcct ttcgatgcgg gatcggtcaa
     8881 ggatattgag cgcgcgattc gtgcctcaaa cctgggcctg aatcccaatt ccgatggaaa
     8941 ggtcattcac ctgaacctgc ctccgcttga tgaagaacgc cgccgcgacc ttgtgaagca
     9001 gatgagtcac cgccttgaag aggcccgtgt ggcagtccgt aacattcgcc gcgacttgca
     9061 taacgacttg cgcgattatg aaaaagaaaa actcatcacc gaagatgacc tcaaacgcgg
     9121 cgaagaagac ttgcagaaac tgaccgacaa atttattgaa gatatcgcca gacacggcgg
     9181 caacaaagaa aaagagatta tggaagtgta gcatcttttg agatgcccct ttggcatcaa
     9241 gaagagtgtt tcaaacatgg ctgagatccc atctgcgatt ccccttgaaa aaattcccca
     9301 acacgttgcc atgatcatgg acggcaacgg gcgctgggca cttcaacgcg gcttgccgcg
     9361 tctggcaggt cacaaagcgg ggacagaaaa tctgcgccgt gtcatacgtg caagcgtgga
     9421 atttggcgtt aagtacctga ccatctacgc tttttccacc gaaaactggg gcaggccgcc
     9481 cgaagaagta aaagggttga tgtatatcct tgaagatgtc attgaccgcg aattgaatga
     9541 attaaacaag gaaggtgttc agctgcgcca tatcgggcgc ctggaaaggc tggcaccctc
     9601 cttgcaggaa aaagtgctgg atgcgattga agcgacaaag aacaataacc gactggtttt
     9661 aaatgttgcc ttcaattacg gtggacggga tgagatcgtc cttgccattc aaaaaatgat
     9721 gaaggatggc atcgccccgg agcaggtgac cgatgaattg gtcagtcagt atcttttcac
     9781 tgcaggtgta cccgacccag acctgatcat ccgcacttcg ggtgaattgc gcatcagcaa
     9841 ctttctgatc tggcaggcgg cgtacgccga gtggtatatc acccccacct tctggcccga
     9901 ttttgacagg gaagaatatc atcgcgcgtt ggaagccttt gcccaccgcg ataggcgtta
     9961 tggaaaggta tcttcggggg aacttcagga atcaaatgcg tagaagacta attactgctc
    10021 ttggattggc tgtaatagga gtgcctgcca ttctattggg aggcatcttt tattacctgt
    10081 tgatgggaac ctttttgatc ggtgcggcat gggaatatgt gcatatgttc cgtgccgtga
    10141 agttcgaacc tcaaatgcat ttgaccgtgg gcggcgtggc tctggtgaca ctggcacgca
    10201 tgttcgctcc tgaattcgca atgcctgttt ttggcgcagc catccttgca gcattgacct
    10261 atcatctcat cgcttatgag cgcggccgtg atcaggccgc gctcgatttt tgcatctcca
    10321 ttggcggtgt ggtgtacatt ggctggatcg gttcctatct gtttgacctg cggaatcttc
    10381 cgcaaggcgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gggttgaagg
    10501 attcgagcga tatcatcccc gggcacggtg gtttttttga ccgcatcgac tcctggttgt
    10561 ggggttcgat gatcggtttt tacttcctgg tttggttcat cctgtagaga tttggaggca
    10621 aaaatggaag ttcgcggcgc tacacctact cgtaatcttg cattggaact cgcacgtgtt
    10681 acggaagcag cggcaatgtt ggcaggtcgt tatatggggc gaggagataa agaaggcgct
    10741 gatcaggctg cggttaatgc gatgcggctt gtcctcagta cagtggatat gaacggggtc
    10801 attgtcatcg gtgaagggga gaaggataaa gccccgatgc tgttcaacgg tgagaaagta
    10861 ggcaacggtt cagaacctga cgtggatgtg gctgtggacc caattgacgg gactcgccct
    10921 ttggcatttg gccgctcaaa ttctctggcc atggtggcgc ttgccccgcg cggaacaatg
    10981 tttgaccctg gcccattcct gtacatgaat aaacttgcag ttggacctga agcaaaggga
    11041 aagatcgata tcgaaaagcc catctccgaa aacctgaaag ccattgcaaa ggccaagggc
    11101 aagcagatcg aagatctgac cacgatcatc ctggatcgtc cccgccatga tgatatggtg
    11161 gctgaaattc ggaaagcggg tgcgcgcatc cgtcaaattc ctgatggtga tgtggccgcg
    11221 gcattgatga cctcctggcc tgattcaggt gtggatgttt tgcttggtat tggcggtacc
    11281 cccgaaggcg tgatcaccgc ctgtgccttg cgggcaatgg gcggcgagat tcaaggcaaa
    11341 cttttcgcgc gtgacgagga tgaattgcgc cgcggacgtg aagcgggtta tgactttgaa
    11401 aaagtgttga cgatggatga cctggtctcg tcggaggatg tgttcttcgc cacgacaggc
    11461 attaccgatg gcgaattgct tcagggcgta cgatattacg gcaatcacat caccactgat
    11521 acccttgtgg tgcgcggttt gacaggcacc attcgccgca tcagcgccac gcacagcacc
    11581 gataaattgg acaacctgag ttcggtacat tattaatttc acttgacatt ccacggcctt
    11641 gtccgtataa tgtcggtcgc tttcctgact agctcaatcg gcagagcgag cggctgttaa
    11701 ccgctaggtt ctaggttcaa gtcctaggtc aggagccaac aaagtccctc atcaattgag
    11761 ggacttttac tttaaaaaag tcccctccgc agcggtgagg gcgccgagga ggggggagaa
    11821 gtgggcttta tgaactaatg ctgacccaat gtcccacgtg agcggacgtt gcgctcgacc
    11881 cgataaaaga accatgagcc gaaccacaac atgaggaaca tggtgccgat gagcagggca
    11941 acttcaacgg gtaacggcag gagcgtattg gttttcagta tccaaccgcg gacggcatcg
    12001 aagccgtagg tgaggggcag catcaatgag atggggatca gccagtaggg gagcgacttc
    12061 acggggaaat ttgcacctgt aagcccttga acaagaaaac tacttacatc caccatggtg
    12121 tttgcgtcac gcatcaccag cacgatggcg gcaaaggcga agccaaaacc gtacaggccg
    12181 atcagcatgg gaattgcggt cagaatggcg gcgatgatgt tgcctgtggg tttgaaaccg
    12241 aagagaagcg cgccaatgat cagcatgacc gtggaagtga tggtcgtggt caataaactg
    12301 gtgagggtgc gcccgacaag aatgtgcaga cgtgaaatgg gagtcagcca attggattcg
    12361 agcacgccag catccatgtc ttctttcagg gcatacccca tgccccagaa caccgtcatg
    12421 atgaaattgc tcagtgcggt gccgatcaaa atataggaca tgtaatcgcc gctgccagtg
    12481 tagccagcga atcccagcgc ctgtccattt gtgctgaagg ctttgcccat gaaataaaca
    12541 ggcgtgaccc agatcaacgg ttgaaagatg ctcgaaacgg cgttcaacgg ataacgccag
    12601 aagaccttcc agtctttgcg ggcgatgacg tacaaggcgc ggagttgcgt ttgccagttt
    12661 gtttgagaga gggtggtgat ttccataata tcctcttagt attgcccgat tgcaccagtg
    12721 gttcttgcgc ggcgttccat ccacacaaaa agagagtatc caagggccag ccagaacaga
    12781 ccgaataaca gaatgggaat caggtcgggg gcgagttcgg gaatgcctgc attgttgaac
    12841 gctgcggcgc gcatcccgtg gatgagatag gtctgcggca tgaatttggc gatgccttgc
    12901 atccagccag gcagcaagct gacagggaag gtgattccgc agaagatcat caccaatcca
    12961 cggacgagga atacaaatgc attcgcttcc ttcatggtga tgacgacact cgcaaaggcg
    13021 aagcccaagc cgtaaatggc ggggatcgca gcgagcatca tcaagccgat catccacggg
    13081 ttgccgttca gccgcacacc aaagaacacg ccaaactcaa aagcagtgat cgagacgaac
    13141 aggagcatgg aaaccaattg cggccccgtg tgtccaagca ggtaggcaaa gcgccaggta
    13201 ggggagagcc agttcgattc gagcgtgccg cgcatctgtt cctgccgcag cgcgaagcct
    13261 acatcccaaa gtacgatgtt ttgccacatc cataccgtgg tgccgacgac gatgtagccg
    13321 atgtactcag ttgcgcctgt ggtgttgagg aagacctgca atccgctgcc atcggggcca
    13381 gagagagcgt gcgccgtcag gatatacatc atcgggaaga tcagcggcca gatgaacatg
    13441 gcgatgatcc atgaggggta acgcatggtg atcgtccact cgcggcggac aaccgcccaa
    13501 agagcgcgca ggtcagatgc gaagccaggc ctgccgagag attgagggga aagggttgtt
    13561 tgcatatcag tctcgcagtg ccttgccagt gaggtgaatg aacacatctt ccagtgaagg
    13621 cttgacgata ttcatattga caagtcttgt gccgttgccg ctcagtgatt ccaccacgcg
    13681 aggcagggcg gtgcggctgt tttgggcttg catattcact tcccatagat ccgcttcgcc
    13741 ttgtttgcgg gtggtgagcg tttcgattcc tgaaatgcct ttcaacttat cgctcaggtc
    13801 atcgtgccag cctgtcaatt cgagtttgat cacttcgcgc tgatcgatct tgcgtttgag
    13861 tccttcgggc gtatcgagcg cgataacttt gcccgtgtcg atgatgccga tgcggtcgga
    13921 gagttgatcg gcctcttcca tgtaatgtgt ggtcagcagg atggtatgtc cttccgcttt
    13981 caaagtggcg atcaactctc ttaagttgcg agccgcttgc gggtccagcc cgagcgtggg
    14041 ttcatccaaa agaagaatgg gtgggcgggc aagcagcgct ttggcgatcg cgatccgttg
    14101 gcgcattcct gtcgaatatt tttcaaccaa ttcattggcg cggtctttga tctccatgcg
    14161 ttcgagcagg tcgtccacgc gtttctttgt gatcgcggcg gggatgtgat acagcgccgc
    14221 aaaatattcg aggttctcgc gcccggtcag cttccaataa atgctgcgct cgccagccag
    14281 caccgtgcca agactgcgcc tcacatcgtt tgcctgcgca accacatcaa agccgttgac
    14341 ctttgcagtg ccgctggtgg gttcgagcaa ggtacacaac atgcggatgg tggtggattt
    14401 gcccgcgccg ttcggcccca gcaagccaaa aatttcaccg cggcggattt gcagatcgac
    14461 tccgttgacg gcggtgaaca tggcctgcgg ctctttcttc caaaattgcc agcgcttctt
    14521 ttttgaacct tgagcggatt cagtctcgac agctttatct cccgtccccg ggcgggcggg
    14581 gaacttcttg acgagttggg tggcttctat ggcgaaatca gacatggggg actccttttt
    14641 gttgaacttc aagaaaatga aggtgacaat tcgtaatatt gacttttttg tttgcttatt
    14701 ttcaatattt tacgaaaata ctttgatttt gtcaatgccg tcatttataa aatcaaaggt
    14761 gggctcaatt ttattgaaca cattgtctgt agggttagac atctcacgct gcgcccccac
    14821 atctaggtga caaatctctg ccttgcgaat aaaataccaa tccatgtggg gacgactgat
    14881 tcgcgacccg aagggagatt ttgatgaaaa ttctcaaaga aagaaaaggc ctattcgtaa
    14941 aaatactggt cagtgtctta tttgtattcc tgtgttatgg attgtcggaa aaggcgttcc
    15001 attgggcggt gtgcggagaa aaagagcgca aagaattaga tatgttgttc ctaatgtttt
    15061 gcgaaaaaga agcagatgca tggtttgaaa aacggttttg ggaggaatgc accaaaaatg
    15121 acaagaacct gccgcctctt acagaacttt tgatttctgc ttgcaaagaa cctgctgtgc
    15181 gtggtttttc cgatggagaa aaattatttg tgattgaggg taaaactgac gaagtttatt
    15241 tcttggattt gcgcacggga gaaagggcac ccgtcccaga tggtaaacaa ttgtgggatg
    15301 aggttttcct gagtccggat ttggtctggt ttgatggata tttaaagcct ggtgatactg
    15361 ggtacatacc ccattacgtt ttggatttga cagacggaaa gaagtacgaa ctttttgatc
    15421 tatcctggat gccgttaaag gatggcaaat ttgataccca aaactatgga tatattaaat
    15481 ctgccgaaca tgtttatttt tcgccgaaag aaaatgctgt aattgccttg gcgcctaact
    15541 ttaggcaaag ccccagacaa aacgttatat attttgactc atcccttgag aaattattga
    15601 acgatctggg ggtaaattat gaactcgtgg attactcgct tgctgacgca gatgtcccat
    15661 ctccaacagg gaagtatgtg gttcgtaacg atgggaacgg gatatatttt tcgggaacta
    15721 atctccccat aataactggt gcgtatagta gcagttgttt caaaagttgg tattacgacg
    15781 aaagcgctgt ggtcattgcc tgtggcattt cgggctatct atatcataat tctattacac
    15841 atagcgtaag tcaccccatt cctctccccg ttcttaaact gtttttgcct actccatgag
    15901 aagaatcatt acccgccatg cgtagcgcgg gatattatcc cgcgctacgg tttgttgcgt
    15961 taatttcttc tctacggcca cggaatttcg gcggggcctg cgaagccgtg tttctcctga
    16021 aagaacaccc tgccatgtga cggttcggcg cacccgcctt catccacaaa ggtgacttcg
    16081 gggtaattgg atgccagtgc ttcgaagacg atctgtccgc cctcacagcg ccatacttcg
    16141 atgatgtaaa tgaacatatc ggcatcttct tcatcaccag cgcggagttc gacatcggtc
    16201 catttcacag tgacagtgcc gccgttattc tcggcgctca acacggctgg gggagcataa
    16261 taaggcgact cagggagttc atattcgccc gggtaggtca cacgcagagt ttgcggatca
    16321 ccttccacac tgatgaattt ttggttgacc cagcacttgt gctccagtcc gtcgaccatc
    16381 acccagttgc tggcatgggc gcgaccgatc aaggtaagtt tgaaatcttt tctgaaacca
    16441 taaaagtaaa gatattccgc gccggggccg tagcggcaac ttaacgtgtc ggctgtgaca
    16501 acggccttca aactatccac agtgggaatc tctgtgggcg tgctggttga cgaagccgtg
    16561 ggcgggggca gggttggcgt gaatgtttca gtgggtggga tgggggtggg cggaatggcg
    16621 gtttccgtcg gtgcggcgac ttcgagcgcc gtggctgtcg ctgcttccac ggcaggtgcg
    16681 gcttcggcgg gggcattcgt cggcgcagag gaaggcacat tgcatgccgc cagaatcagg
    16741 accagggtta aagtaagaac ggggtgtatt ttcatgatca agtctttttc cattccataa
    16801 atttgatttc ttcaacatcg ctggtgggca gcaatgtgat tttatcctta cgcccttcac
    16861 ggatgtaaat tttgtaaact gcgtctgctg gggtgcggac ctttgccaca agattgacct
    16921 ttttatttac aactgcaagc aggtcgagcc gtttgacaaa aagaaaggat gattcctttt
    16981 cgaatgcgat caaatctgcc ttgccgaata accagccaac cttgccgcgc acattgcgaa
    17041 gttccaccca ggtgaaatcg gtctgttcgg ttttatcgcc cctgcggatt cgtttctggc
    17101 ttttcacttc cactttgaaa acctcgcctg ctttttcgat caggtaatcc cagtggtcgt
    17161 caatgttctc atccttcgat gaggccgaaa ccttccagcc tgatctgacc gccagcacga
    17221 tgaaaagatt ttcagcgtgt tcgccaagtt ccagcgaatc ttttgtgtcg aatttgttgc
    17281 gagagttggc catgctattt tttctttttg ggttttacag gtgcgggcgt aatgcccagg
    17341 attcttttgc gcgccagttc gcagtatttc ctgtccacgt caatgccgat gcctttgcgg
    17401 ttgttattca ccgctgccag cagggtggtg ccgcttccgc tgaacgggtc gagcactaca
    17461 tcatccacat aactgaaaag cttgatgcag cggcgcggca attcaagcgg aaaaggggcg
    17521 gggtggccga ttcttttttt gctttcgccg ttgaaggtcc acaggccgtt cgtccaatcc
    17581 atgaactcgt ttcgttcaat gtctgaaatt ttgctgccgc tggttttctt ccaattgttt
    17641 ttgtacagca cgatgatcaa ttccacagga gcgatcacgt acggtgcgga tgcacttttc
    17701 catgaccccc aggcggttct gcgcgaaatg ttgccctcgt tccaaatgat ggtcgagtgg
    17761 tagttgaacc cgatctgttt tgcgatggtc gttagatctg cgcccacact ttgctgcccg
    17821 cctttgtttt tatcgagcgg cacattcagg cagaagcggc catcgtcttt gagccagtca
    17881 aaacagcgcg acatccattt tttgctgaac tccaaataat ccgcatacga aacgtcgtcc
    17941 ttgtgcgaat tgtattggat gtccacattg taaggcgggg aggtaacaac aaggtccacg
    18001 ctgtttgcgc tcacgagcga tgtggtcaaa atatcgtcgt gaacgagcgt gaacctctcg
    18061 ttttgaaaat ggattccgaa tgctttcatg ctcggattat agccgacgcc ttatccgctt
    18121 tgtgaatcaa aattgccttc caaaatcttt tcgatgaatt gtttgaaaac ggaaaagggt
    18181 tgcgcgcctt cgaggatgtg accgttgatc agaaaagtcg gcgttgcgtg gactccatcc
    18241 gcttcagcct gcgccttgtc ctgctccacc gtccctttgt gagttccctc gctgagacag
    18301 gtctcgaacg catccacatc caggttgacc gctttggcat atttgatcag attttcctgc
    18361 gtaaaatcgc ctacgttttc gcctgtccag ttggcataga gcgtgtcgtg gtactcccaa
    18421 aacatgccct gatcccccgc acagtaggcg ccttccgctg ccaaaacaga ttcaggccca
    18481 aggaacggga atgagcggta ttcaaaatag accttgcccg tgttgacata ctcctcgatc
    18541 aaaagcggct cggtctcgtt ccagaacctg aggcagtatg ggcactgaaa atcgccatat
    18601 tcaatgatgt ggatgggcgc atgcggatcg cccatcgtat tggcctgtat cactgcacgt
    18661 ggaagttcct ccgccacttc caccttgtca ggaaagaact gctggacaaa ttttggcccc
    18721 acaaagatca gcgtgccgac aaagaccgcc gcgcaaagca gcactgcgca tccgcctgcc
    18781 gcaagccata cccgccagtt cgttttttgc ggctcaacga tcacaggttc attttgtgtt
    18841 tcgggtgtgg gctggttttc aagtgggggt tgttcttcga tcatgattct tcctccgggc
    18901 ggtatctttt tccgttgctt tttctctgtg tcatctgtgg tggatgtttt ttttgtcgtt
    18961 tcgccactcc ctttttggag cggacattca atattccctc agacgccgaa tgggggaatt
    19021 cgattacctc accacaactt ccttatctaa aactcatacc cacatcataa accgaaatca
    19081 ttttttcgat ataatttcac catgaatgcc acccttacct ccaaccagaa tttacccaaa
    19141 cagatcgttg ccgcacttgt cggcggattc tcgcttttcc ttaccgttac catcacatgg
    19201 accctgggct atcaattatt gtatgcgggc cgcattttcc ccggtgtttc tgtggcaggc
    19261 gtggatgtct ccgggctgtc catcaatgat gctgcgttga aattgagcca gaccctttcc
    19321 ttcccgaata cgggcaagat tctgtttcga gcaggcgata agctttgggt tgcatcccct
    19381 gcagaattgg gaatggtgtt cgaccccaca gccagtgctc tggcagccta tcgctatgga
    19441 cgttccggcg gcttgtttgg tgcgttatcg ggtcaggtca gcgcccgtgg acttggctca
    19501 gatgttgccc cggtcattat tttcgaccaa cgggtcgcct acacctattt gcaaaatatt
    19561 ggggcacagg ttaaccaacc tgtagtggag gccagcctgc accttgaagg gacgaatgtg
    19621 gtggcccagc ccgggcaggt gggcagagtg ctcaaccttg atgccactct catctacctc
    19681 ggcgctcaat tgcaaacctt ccgcgatggc gaagtgccgc tggtgattca ggaagccgcg
    19741 ccgaaactgt tggatgtctc ttcacaagcc gaagccgcac gccgcattct cagccagcca
    19801 ttgaccctca gcctgcccaa ttatcgcagc ggtgaccctg gtccgtggac ttatgatgtg
    19861 cccgtagttg caaacatgat cgcggtgatc gttgtcgaaa atggcggcgg tgccgaaatg
    19921 caagttgggt tggatccgaa cattttgcgg caatcattga acgaactcaa aacttacgta
    19981 gaccgccagc ccacgaacgc gaaattcgtc ttcaacgatg gcaccagcca acttgaaccc
    20041 attgcggctt ccaatgtcgg ccgcgccatg gatgtggaag ccagcattgc cgccattaac
    20101 gatgcgttgt tgcgcggcga acacaacgta tccctcgtcg ttgctgaaca gcagcctttg
    20161 gtggcagata cagctgtcgg cgcagatttg ggaatcgtgc agctcatcgc cgaacaaacc
    20221 acctatttct acggttccag caccgaacgc cgtcagaata tcgaagcggc cgccagtcgt
    20281 tttcatggtg tgttggtggc ccccggcgaa accttctcaa tgggcagtgt gatgggcgat
    20341 gtcagcctcg aaaatggatt tgccgaagcg ctcatcatct atggcgggcg caccatcaag
    20401 ggtgtgggcg ggggagtgtg tcaggtcagc accacgttgt tccgcactgt gttttttgcg
    20461 ggctttcctg tggtggagcg ttattcgcat gcctatcgcg tttcttatta tgaaaagacc
    20521 tccagtggct ctgtagactc aaaccttgca ggcatggatg ccacggtgta cttcccgttg
    20581 gttgatttca aattccaaaa cgacaccccg tattggattt tgatggaaac ctacatgtct
    20641 gaaaacagcc taacctggaa actctattcc acctccgatg gacgcagtat cacatgggag
    20701 accacaggcc cgaccaatat tgtccctgcg ccgccgccgt tgctcgaaga aaaccctgaa
    20761 atgaaagccg agcagatcaa acaggtggat tacgctgcac agggtgcaga tgtcaatgtc
    20821 acccgcgtgg tctggcgcaa tggagtgatc tatttcaccg atcaatttat cacccattac
    20881 caaccatggc aggcgatttg tcagtatggg ccaggcaccg aagacccgaa aaaactggca
    20941 aggcgaaacg acatttgcgt tggagccagt tagctgaaca aaatgatttt gattgcaagg
    21001 ctttgtggta ataaaacgga cttttacaac ggatctggag tccgagctca caggcttttc
    21061 aagatttaag aagaaaagcg ggaaaaaccc atcatattcc cgcatggtac aatccttccc
    21121 tgaaaaggag aatacatggt cagctcagaa ctgctcgaaa ttttacgatg ccccaattgc
    21181 gtgcgcgaaa aaagcggcac gctggttttg cacaaagatg cctggcttat ctgccaggat
    21241 tgcggtcgca aataccccat tgtggatgac atccccgtga tgttgattga cgaaggcgat
    21301 aagtgggtca acaccgcaca agacgccctc cccgtccccc cgcctgaaaa ataaaggttc
    21361 gcttgagcga agctaaaatg caggaactga tcgccgagct tctcagcggc gatgatgaac
    21421 gcgccgaaaa aaccatccct gcccttgtgg gttttggaat ggcggcgcat tctgcattat
    21481 tagacctgac ccgtgccgca gatgtggatt cgcgctggtg gggggtacgt gccctcgccg
    21541 cctcgcctca cacacggacc gaagacctgc ttccgcttct cagcgactca gcgccggaag
    21601 tgcgtgccgc caccgccctt gccctgtgca atcatccgca cgaggcggct gtggaggcgc
    21661 tggttcaagc ccttgcggac gaagacccgc tcaccgcagg gttggcaggg acagccctcg
    21721 gcaagatcgg tgctccttcg gtgccgagcc tgctcaaggt gatgagcgaa gcgccggttg
    21781 gcgtcaaaat tctggcgttg cgggcattat ccgaaattaa agaccatcgc gccatccccg
    21841 tcatgatgaa atcaatgggg gaagaatctg cgcttttgca atattgggcg caggaaggct
    21901 tggaacgact tgggctaaat atggtatatg ttaagccctg aggtacagaa aacaatgaat
    21961 cgatttgaat tcctgaagaa aataagactg cccaaaccaa aaatcccctc ggtgggacgc
    22021 atcatctttt gggccatcag cattgggttg gcggcagcga catttgtctt tgcacgcaat
    22081 ttcacctcct gctggaacat caccggcctg cctggcattg ctccctccag ttgcggcaca
    22141 tcaaccaacc cgctggctgg cccggatttg acggcagcca cccaggaacc cggtgcagtg
    22201 gatacactgc ccaccccggc tcccgtggct cccggtgtgg aattgccccc cgcatgggat
    22261 ggtggcagcc gtatcaacat cctcttcatt gggttggatt tccgcgattg gcaggccaac
    22321 gatggaccgc cgcgctctga tacgatgatc ctcttcacca ttgacccggt cacaaaatcg
    22381 gcgggtatgt tgtccattcc gcgcgatttg tgggtgaaca ttccagaagg gtttggatac
    22441 agccgcatta atacagcata ctccagcggt gaaggcgcaa aattgcccgg tggcggccct
    22501 ggccttgcca tgaaaaccgt agagcagttg ctgggtgttc ctgttcatta ctatgcccaa
    22561 gtggatttca acactttcac cgaattggtc aaccgactcg gttgtattta tgtcgttcca
    22621 gaagaaattc ttgtgcttga ccctgtcggc cccggtacag accatgtggt catcaccccc
    22681 ggcggcgaac gcaaactgtg cggctggaaa gtattggcct atgcccgcac ccgtaaaacc
    22741 gaaggcggcg atatggaccg tgcccgccgc cagcagcagg tgatcttcgc tcttcagcaa
    22801 aagatcttcg accctgaggt tttcccctct ttgatcgcgc aggctcctga aatgtacgcg
    22861 gaactctcca gcggtatcca caccaacatg cctctggata cggccatcaa actggctatt
    22921 ttgggcaaag acatcactcg tgaaagcatc aagaccggca tcatcggcac cgatatggtg
    22981 acctttgaaa gcaccaccct cggtggacag gctgccagcg tcatgaagcc gatcatggat
    23041 aaggtccgcg tgttgcgtga tgaaatcttc accagcggcg gccccaccag cccnnnnnnn
    23101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttatcgt cactggtaac atcaggagac
    23221 attggtgctt gagacatgat ttattcctcc gaataagttg attttggatt tccagtattt
    23281 tacaaccatt cccactatca tgcaaccaat cctcttcacc caagagtact ggattgaatt
    23341 ttaaagcgca aaccatgttg ctgatcctga aaaaaattca acaagcggta aaatcaaagg
    23401 atgacattac gaattgtttt catgggttcg cccgattttg cattgccttc tctttccaca
    23461 ctggccgccg aaccgaatta tcaagtggtt ggtgtggtca cccagccaga ccgcgcctca
    23521 gggcgtggcc gtgagttgaa accacccccg gtcaaatccc ttgcgcttga attgaacctg
    23581 cctgttatgc agcctgaaaa gctgcgccag cccgaagcca tggaacagtt acgtgcatgg
    23641 gagccagatc tcattgtggt tgcggctttt ggtcagattc tgaaaaaaga cgtattggat
    23701 ttgccccgtc atggctgtat caatgtgcat gcttccctgc ttccgcgttg gcgcggcgcg
    23761 gcgcccatca acgccgccat tttacacggg gatgaagaaa caggcgtgac catcatgcaa
    23821 atggatgtgg gactcgacac gggccccatg ctggcaaaga ggtccgtgcg catcaaacct
    23881 gacgatacag cgggttcggt ttcacaggca ttatccacac tgggagcaga cctgctcatc
    23941 gagaccctgc ctgcctacct tgcagggaat ctcaccgcca cgccgcaacc cgaggatgga
    24001 ttcacgtatg cgccaatgtt gaaaaagacc gacggcctgc ttgatttcac ccaacccgcc
    24061 attgacctgg aacgccgtgt gcgggcgatg aatccctggc cgggcgcatg gtttgagtgg
    24121 aatgggaatc cgctcaaggt gttgcgcgca tccactgtca gcggcgaaaa aggcttggca
    24181 agcggcagca ggtttacggt cgaaggaaga cccgctgttc aaacttccgc tggcgccttg
    24241 gttttggatg aaattcagcc tgcaggaaaa aaggcaatgc cgggcaaatc ctttctggca
    24301 ggcgcacggg attgggttgc agttgcgtag cgtatcgaga ccccacgggt tataatctca
    24361 aatcatatct ttttggagaa gaaaatgttc aattcgaaag tgtttcttgc cgaattcatc
    24421 ggcacattcg cgctggtgtt tatcggcatg ggtgcaggca ttgtgaatgc aggcctgttg
    24481 ggtgtggctc ttgcacatgg atttacgctg gcggttttcg cttacgccta cggccatatt
    24541 tctggaacgc acgtgaaccc cgctgtgacc tttgcactcg cactgaaaaa tgttgtcacc
    24601 tggggtcagg ctgtggttta ttggattgcg cagtttgcgg gcgcgatcct ggctgcattt
    24661 gtgatgaaat tgttcctcga ttctttgggt ggccccattg atgctggcgc gaccaccggt
    24721 gcgctcaccg ataaggctcc cattttggcg atggtgatgg aagcgatcct gaccttcttc
    24781 ctcgtcaaca cgatcttgca cactgccgtt ggcggcaagg gcggacgctt tgctggttgg
    24841 gcgatcggca ccacattggc tctggccatt ttgggcggtg gacctttcac cggcgcttca
    24901 ctcaaccctg cccgcaccct gggaccggct gtctttgctt ccattccgag ccttaccagc
    24961 ccaaacacct atctgatcta tctgtttggt ccgttgatcg gtgcaaccct ggctgtgttg
    25021 gtttataact tcttcaccag ttccgatgaa attgaagagg atgaagaaga agttgtggaa
    25081 gaagtacctg cggttgttga agaagaagct cctgtgaaga aatcccgctc ccgcaagagc
    25141 gaagagtaaa atccgcaaaa ctggggggat ggcgggtagt aaacatcagg nnnnnnnnnn
    25201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttttttccga ttgataaaaa tctatgctaa
    25321 aatctacggc ctttgtaatt tcccggagat taaagcatgc aagatgattt actggctgca
    25381 aagccgcccc aaaacacttc aatggacgat ccgatcgctg cgcggcggac ctttgccatt
    25441 atttcccatc ctgatgcggg caagaccacc ttgaccgaaa aactgctttt gtatggcaat
    25501 gccatcgaac tggcgggcaa tgtccgtgcg cgcaagaacc aacgttcgac cacctctgat
    25561 tggatggaga tggagcgcga gcgcggcatt tcgatcactt cgaccatttt gcagtttccg
    25621 taccaaagca atattgtcaa cctgctggat acccctggac atcaggattt ttctgaagat
    25681 acctatcgca ccctttcggc ggtggacagt gctgtgatgg tgatcgatgc cgccaaaggc
    25741 atcgaaccgc aaacgctgaa attgtttgaa gtctgccgca agcgtgggat tccgattttt
    25801 accttcatca acaaaatgga tcgcccggcc cgtgatccgc ttgaattgct ggatgagatc
    25861 aaaaaagtgt tgggcatgga acctgtgcca atgaactggc ccataggcga cggccccgaa
    25921 ttccgcggcg tgtatgaccg tgcctcgaaa ggtgtgtatt tgttcgagcg caccgaacgc
    25981 aatgaacgca tggcgcctga gatattcgtt ccgctggatg aacttggaaa gaacaatatc
    26041 ctgccgcaga aacgctatga cgaactggtg gaaagcgtcg ctttgctgga tgaagtgggt
    26101 gtggttttcg accatgacct tgtattgcag gaaaagcaaa ccccggtctt ctttggcagt
    26161 gcggtcacca actttggcgt gcgcctcttc ctcgatacct ttgtgaaata cgcgcctcaa
    26221 ccccagccat acttgagcga taggggagcg gttgtgccgc aagaccctgg cttttctggt
    26281 ttcatcttca aaatacaggc gaatatgaac ccaaagcacc gcgacagtgt ggccttcctg
    26341 cgtatttgct cgggtcgctt tgagcgcggc atggacctgg tccatgcgca gagcggcaag
    26401 accgtgcggc tggttcgtcc gtataaactg tttgccagcg aacgcgagat cattgacgaa
    26461 gcctaccccg gagatgtgct tgggattccc aacaacggag aatttgccat cggcgattct
    26521 ctctgtacgg gggagctggt tcggtttgcc ccgatccctc gttttcaacc tgagcatttt
    26581 gcactgcttc gcaacacaga cctgggcaag cagaagcagt tccaaaaggg actcttgcaa
    26641 ttggaaagcg aaggggccgt gcaggtgttg tttaatgtca atgccttcaa gcgtgagccg
    26701 attctggcag tggtcggtca attgcagttc gatgtggtgc aggcccgttt gaaagcggaa
    26761 tacaatgtgc ccaccgagtt ggaacgtctg ccgcatgtga tgatgcgctg gatcaaagga
    26821 cctgaaacgg cgatccaaaa tatcacccaa cgcagcgaag tcatcattgc gcgcgattcg
    26881 cgggaggaat gggcggcgat tttcagcacc ccgtttttcc tgaagtactt catcgagaag
    26941 aatccagatt tgcgctttgt ggaaattaca gagaattaag taaaacggcg ggtttcaacc
    27001 gccgttttga tttaaagatg aaagtctgtt gaagtgtggg gcagggaatg cctgaaaacc
    27061 cagccgcgaa gattttttct ttgcggcttg atgtctttat agagaaattt gtgtttctga
    27121 tacatggtca ttttttcgtg gttttgtttt acaattaacg ccaaggaagg atgccatgtc
    27181 aaaatatgtt gctgccatag atcagggtac caccagtacc cgctttatca tcttcgatca
    27241 cggggggaat gttattgctg ttgaccagaa agagcatcgt caaatttacc cgaaaccagg
    27301 ctgggtggag cacgatccgc ttgaggtttg ggaacgcaca caggaagtca tgaaaggcgc
    27361 tttgcaaaag agcggaattc aagccgcaga tatcgccgcc attggaatca ccaatcagcg
    27421 tgaaacggca gtggtgtggg ataaatccac ggggaagcct gtgtacaatg ccattgtctg
    27481 gcaggatacg cggacggatg tcatttgcaa cgaactcgcc aaggtgggcg ggcaggacag
    27541 actgcgttcc aagacgggac ttccgctggc aacctatttt tctggcccca aaattaaatg
    27601 gattttagat cacgtggaag gtgcgcgcgc caaagctgaa aaaggcgagt tgctcttcgg
    27661 caatatggat acctgggtca tctggaattt gaccggggtc cacgtcaccg atgtgaccaa
    27721 tgcctcacgc acattgttga tgaatttgaa caccctcgat tgggacgatg aacttttaaa
    27781 tctgctggat gtgccgcgcg cgatgctgcc tgagataaag tcatcttcgg aagtatatgg
    27841 ccatgttact caaagcggct tgagcgccct gcaagggatc cctgttgcag gtgacttggg
    27901 cgatcagcag gccgcgcttt tcgggcagac ctgcttcagt gcaggggagg cgaagaacac
    27961 ctacggcaca ggctgtttca tgttgatgaa catgggcgag attcccgtcc cttcgaaatc
    28021 ggggctgttg accacgcttg ggtacaagat cgggaatcaa aaagccgtgt atgcattgga
    28081 aggttcgatc gccatcacgg gggcgttgat ccagtggctg agagataact tggggctgat
    28141 ccagtcgtcg gcggaggtgg aatcgttggc ttcatccgtc gaagataatg gcggcattta
    28201 ttttgtccct gcgttttcgg ggttgttcgc tccgtactgg aagtcggatg cgcgtggggc
    28261 aattgtcggc atgacgcgtt acgtgaccaa gggtcacatc gcgcgggcgg ccctcgaagc
    28321 gaccgcctac cagacctgcg aagtgctcga cgcaatggaa gccgactcgg gtgtgaagtt
    28381 gaccgcgttg aaagtggatg ggggcatggt gttcaacgaa ttgctgatgc agttccagtc
    28441 ggatattttg aatgtgcctg tggtgcgccc gaaggtggca gagacgaccg ccctcggcgc
    28501 ggcttatgcg gctgggctcg cggtgggttt ttggaaggat tatgacgaac tccgcgccaa
    28561 ctggggcagg gacaaggaat ggactccgca gatggacaca accctgcgtg gaaaattata
    28621 tacaggctgg aagaaagccg taacacgcac gtttgattgg gttgagtagc gggttgtgag
    28681 tgggttgcaa gttgcaggtt gaatgttgca agtttgaaac ctgaaacctt caacctgtaa
    28741 ccttcaacct ttgaacatga aactggataa taaaaatgaa ccgaaatgaa attctcgaac
    28801 aactgaaagc aaacccaaag atctcggtgt tgatcgtagg cgcagggatc aatggcattg
    28861 gcacattccg tgaactggct ttgaacggtg tggatgtttt actcgtagat cgcggcgatt
    28921 tttgctcggg cgcgagtgcg gcttcttcgc acatggcgca cgggggaatc cgttatttgg
    28981 agaacggcga gtttcgtctg gtgcgcgagg cggtgcagga gcgcaatcgc atgatccaaa
    29041 atgcgccgca tattgtgcag ccgctgccga cgacgattcc gatctttaaa tatttttcag
    29101 ggttgttcaa tgcgccgctg aaatttttgg gctggctcga taaaccctcc gagcgcggat
    29161 cggtcatcat caaacttggt ttgatcatgt acgatgccta cacaggcaag acccgtaccg
    29221 tgccgcgcca tcaatttttt tcacgtgccg aagcgctgaa gaactggccc cgcctgaatt
    29281 ctgaaattgt gaataccgcc aaatactacg acggtctgat ctccaacccc gaacggttgg
    29341 cgctggaagt gatgcttgat ggcgaagccg agaaccccaa tgcgcgcgcg gtaaattacg
    29401 tcagcatggt gaaggctgaa aaagataccg tcattttgcg cgatgaattg accgaccaaa
    29461 cctacgaggt gcgcccggaa ctggtcatca atgcggctgg cccgtggatc gattttgtca
    29521 accacagcat gggtctttcc acccgttcca ttggcggcac aaagggctcg cacatcgtca
    29581 ttgaccatcc tgaattgcgc gccgccatcg gcgaaaatga atttttcttt gagaacaaag
    29641 atggtcgcat cgtcttgatc ttccccttgc aagaccgcgt gatgatcggc acatcagata
    29701 tcaagatcga taatcccgat gaggcgcaat gcaccgatga agaagtggat tacttcatcg
    29761 gactggtggc gcgcgttttc cctgatatca aagtgagccg tgaacagatc gtcttccgct
    29821 ttacgggggt acgtccgctg gcaagcagcg gggcgaagac cacaggtcaa tacagccgcg
    29881 atcatcatat cgaagtgttg aatggcgatt ggacgaatct cggtttcccc atttactctc
    29941 tcgttggcgg caagtggaca tccttccgcg cgttttcgga gcaggtggcc gataaggcac
    30001 tggcgttttt gggaatgccg cgtcagaaag atacgcgcga tcttgccatt ggcggcggcc
    30061 gcggatttgc agttgaccct gatgggcaga aacgtcagat cgaaggcttc gcagcatgga
    30121 ctggcctcga caaggaacgc ctgcaaactt tgtacctgcg ttacggttcg cgcactgaag
    30181 cggttgccaa ttttatgaaa cgcgccgccg atgagccgtt gaagtctctg ccttcgtaca
    30241 cgcggcgtga gatcatgttc cttgcgcagt atgaaaaggt cgcccgtctc gatgacctgc
    30301 ttttgcgccg taccatgttg gccatgctcg gccgccttac caaggaatcc gtgcttgaaa
    30361 ccgccgatgt tctgggcgaa gcactcggtt ggggcagtga ccagaaaact gccgaggtgc
    30421 agcggactct ccgtttgctc cgagacaggc acggggttgt cctgaacgag gccaagtcct
    30481 gagccaaggg cgcatggtag aatcctgtaa tgtcttccaa cacaacagtt gatcttgtca
    30541 tccccgtctt caatgaagaa ggcgtgatcg aacatactca caagcggatc tgcgccgtgg
    30601 tggattcgct tgcttatgat ttcaaaatta tctatgtgga tgacggttca cgcgacggga
    30661 cagttgcttc gctccgaaag atagcagatt cagaccagag agtgatcctg ctccaactca
    30721 gccgcaactt tgggcatcaa gccgcgctga cggctggtat ggacgcctcg caaagtgaga
    30781 tcgcgatcac gctcgatggc gatgggcagc acccgccaga gatgatcccg caaatgttga
    30841 atttgatttc gcaggggtat gacattgtgc aaacccagcg catggacgag gctcaacccg
    30901 cctcgttcaa aaaatggacc tcgggcctgt tctatcgcct gatcaacgtc atcagcggga
    30961 cgcaggtttt gcccggggcc gcagacttcc gcgcactcac acgccccgcg ctcaatgcgc
    31021 tcaaggccat gcctgaatac catcgttttt tgcgcggcat ggtttcgtgg atcgggtttt
    31081 cgatggtcat ccttccgtat caacccgatg aacgcattgg cggtgtttcg aaatactcgc
    31141 tcagtaaaat gatccgcctt gcgatggatg cggtcttttc attttcactt gtgcccttgt
    31201 acgtgggttt gagccttggc ggaattttgc tctgcctggc catggtggaa atggtgtacg
    31261 tcctctcgtt ttgggtcaca gggcagactt cgaatcttgc accgggttgg agttcgttga
    31321 tgttcgtgat cctgatcgtc ggcggcatgt tgatggcctt gttgggtttc attggcgtat
    31381 atgtcggtta tatttttcag gaagtcaaac gcagacctgt gtatctcgtg aaaggggaga
    31441 tgaaacgatg agcgaatccc gtccacagcc ttttcagatt accctgctcc gtcacggtga
    31501 atcgaccggt aatgccgaat cccgttggca gggtcaggct gattttccgc tcaacgatac
    31561 tggccgcgca caggcgcgtg cgcttgctga acgctggaaa tctgaaaaca tgaagttcga
    31621 ttttgtgatc tccagtccgc tgggacgggc aaaagaaaca gcagagatcg ttgcttcatg
    31681 cctggatttg aaagtggagt ttgatccgct ctggttggag cgtgacaatg gcgagtttgc
    31741 aggcctcacc gcgcatgaag ttcggcagaa ttttcagcac cctgcttttt ccacgcccta
    31801 cgatcccgtt ggtgtggatg gggaagggga ttgggagttg ttcctgcgtg cgggtcaggc
    31861 attgcatagc ctgctcaagc gtgagcccgc gcgatatttg atcgtttcgc atggcggttt
    31921 gttgaatcaa atcatgcatg ccgtggtcgg gctggctccg caggccaata atgcgggcac
    31981 ccgtttccgc tttgagaaca ccgcctttgc ccagttgacc tattatccgc accagcatcg
    32041 ctgggcgatc gataaattga atgaccatgc gcattggagg gatgcggagt agacaggtaa
    32101 acaggtttta ttcatccttc atctttcatc cttttctttc cccgaggttc ctttgcaatc
    32161 atccatttcc aatgtaaaag caacagatca attgaaagtc cttgtcttcg gcgcaggcgc
    32221 gatcgggaca tattatggcg ggtcgcttgc gctggccggt catcatgtcg ttttcgtgga
    32281 acagcccaaa atggtggagc agttgcgcga gcgcggactg aggcttgact tgacgatcga
    32341 tgaaagacgg aagacaaaag acgcctttgt tgttgagccg cgttcctttg tgatcgaacc
    32401 ctctttggaa gaggcgctga aattcggtcc ctttgacgtg gcgctttttg cgttgaagtc
    32461 cttcgatacg gtgtctgcct tggaagggat gaagcccttt gccgataaac ttccgcccat
    32521 tctttgcctt tcaaatggcg tggaaaatga acaggcgatt gcgcgcacac ttggcgcaga
    32581 caaggtcatt tacggtaccg tcacaactgc cattggcagg cgtggagcag gggatattgt
    32641 cctcgaaaaa ctacgcggca caggcattgc ggcggggcat gtcctttcgg aaaagctcgt
    32701 tgctgcattt cagaatgcct atctcaattg ccgtttgtat cccgaaccac atggaatgaa
    32761 atggtcaaaa atgttgacca atctcattgc gaatccaact tctgcgatct tgaatatgac
    32821 agcggcgcaa gtctttgcca atcgaaaatt atacaaactc gagatcgcca tgctcaagga
    32881 atgccttgac gtgatgaagg cgcaaaatct cgaggtcgtg gatctgcctg gtacacctgt
    32941 caaattgctg gcgctggctg cgcatctgcc gatctggctg tccaaaccgt tcctttcccg
    33001 cgccgcaggc agtggacgcg gcggcaaaat gccctccttt cacattgacc tgtacgcagg
    33061 ccgcggccaa agcgagatcg aatatctgca tggtgcagtt gtccgcgaag gaaagaaaca
    33121 tggcgtgccc acccccgtca atgaagtctt gactacaacc ctgctggcat tgaccaacaa
    33181 ggaaattccg ctggaagagt ttgcaggtca gccagagaaa ctattggcca acgttttaac
    33241 gttaaaacgt taaaacgttt cgacgttaaa acccatgatt cacctcactt ctcccaatcc
    33301 caatgttgaa ctccatctct ccgatggacg catcttgtca ggtccacgcg gcacacaggt
    33361 gggggagttc cttcgtgcag tcaaagatga ttttagcgct cccattgttg ccgcgatcct
    33421 caacaacgaa attcacgaat tgacctatcc catcgaacgt gaatcagatt gcgcccccgt
    33481 caccatggac cgcgccgatg gcgcgcgcat ctatcgtcgt tcgctgattt tcctgcttga
    33541 aattgcattc gctgaacttt tcccaaaagg caaacttacc attgaccatt ccgtttcatc
    33601 gggtgggttt tattgtcagg tcaccgagcg tgaggcgttg acgcaaaaag aacttgatgc
    33661 gctcaaaact cacatggaaa agatcgtggc tgaagaccag gcctttgaac gcaaacaaat
    33721 tccgattcag gaagccattc aatattttga aaagctcggc tactcagaca aagtccgctt
    33781 gttttcgcac cgccaaaaag attacctcac cctttacagc ctcaacggcc gtatggatta
    33841 ccttcacggc tacatggttc cctccacggg gtatctgcgc tggttcgatc tcaattgcat
    33901 tgatggtggt tttacacttc aatttccgcg ccgccatgcg cccacacacc ttgagccgat
    33961 gggcgactat ccaaaactgc ttaacacctt tcgtcaatac ggtgattggc tgacgcgcct
    34021 cgccattgac aatgtcggcg cactcaacga tgccattgtg gcaggacgcg ccgatgaact
    34081 catcatggtt tcagaagcgc ttcacgagca gcatgtcgct gatatcgccc agcagatcgc
    34141 gcaaaaaaat tcgcgcatta ttttgatcgc gggcccatcc tcttcaggaa aaacgaccac
    34201 ttcgcgccgc cttgcgatcc aattgcttgc gcgcggcatt tcaccgtatc cgcttgaatt
    34261 ggataactat tttattgacc gctcgaaaac tccgctcgat gaagaaggca aacccgattt
    34321 cgaagcgctt gaagcgcttg atctaaaccg cctcgcgcag gatatcgaga aactgctgcg
    34381 gggagagaaa gttcaattgc cgagatacaa cttcaagtcg gggttgagcg agatggggga
    34441 tgtgatccaa ttgaaagacg gccagccgct catcctcgaa ggcattcatg ggatgaaccc
    34501 ccgcctcatc cctgaccgtt ggtctggtgc ggcgtttcga gtatatgtct cagccctgac
    34561 acagctcaac ctggaccgcc acaatagagt ttccacaacg gatacgcgcc tcatccgccg
    34621 catggtgcgc gatgcgcgcg aacgcggata ttccgccacg cagaccatca gccgttggga
    34681 atcggtgcgc cgcggtgaaa agcggcacat cttcccctat caggaaaacg cggatgtgat
    34741 gttcaactct gcgctggtct atgaactcgc cgcgctcaaa ccgctggccg agccgctcct
    34801 gcggcaggtg caacaccgct cgcccgaata catcgaagcg agacggctgc tcgccttcct
    34861 cgaatggttc ctgccattgg atgtgaattt gatccccgcc aattccattg tccgcgaatt
    34921 tttgggcggc tcgagtttga aagatttcaa gatctggcgc gggtagatcg aattccccaa
    34981 ataattagga cgctgttttt gctgactttg gttttttcag cgttaccagc gtcctctttg
    35041 ttttatagag tgcctgaaga ccaaccacaa agccaccaag acacaaaaac acgaagtttt
    35101 tttctttctt tgcgtctttg agattttgtg cctttgtgtt gaaaatctct tctttttatc
    35161 ttctggataa ctcactgctt aggatgtaat gattgtctgc ctgtttttcc aggatgacga
    35221 tctgacaatt cttggggttg tgcaggcttt caaattcctc cacactccaa tgaaaccagc
    35281 gcattaggaa taatctcatc gtcagcccgt gagaaacgat caaaacattc tcggggaaat
    35341 tttctttttc gaaatccctg tgaagggtat cgaggaaact gctgacacgg tcgtacacat
    35401 ctgcaccaga ttcaccatca gggatgcggt aataaaaagt gctgaagtta tcccgctctt
    35461 cgataattcc tttattgtca tctgggtggc gcaagtgtcc ccaatcctgc tctcgtattc
    35521 tggggtcttc aattgatttg acgatattct ggctgatcgc ttctcgaatg agttgaaacg
    35581 tttcgcgtgt tctgaagaag ggagagagat agacgtaaac tttcccgtct tcgaggatct
    35641 ctttgagttg cacgcctgcc agccgagcct gctgaacgcc cgtggaggat aatcgcaggg
    35701 cgaaatcctg aacggtttga tagcgtgtat ggtcaagatt tccttctgat tccgcgtgcc
    35761 gaatcaggat aattcgtttg ggtctcattc caaaactccc gtttgatgct tacttcaaaa
    35821 tggtttcgat ctgctcaagc tctgctgcag tgaattcgag atgcttcaat gttccaattg
    35881 catcgtcgat ctgttccact ttactggcac cgatcagcac ggaggtcatt gaatcgtggc
    35941 gcagaaccca tgccagcgcc atttgcgcga gagtctgttc gcgggattgg gccaattcgt
    36001 tcagctttct cactttattc agcttttcat cggtgatgtt ggcaggcttg aggaagccat
    36061 gtgctttcga tgcgcgtgag ccttcgggga ttccgccgcc gagatattta ttggtgagca
    36121 agccttgcgc aagcggagag aaggcaatgc agccgacgcc ttcttctttc agcgtggcaa
    36181 gcagaccatc ttccacccag cgattgaaca tgctgtaagc tggctggtgg atcaggcagg
    36241 gcgtgccgag ctcgcgcagc attttggcag ccgcacgggc tttttcggcg ggataattgg
    36301 agatgcccac atacaatgcc cgtccactgc ggacaatgta atcaagcgcc tgcatggtct
    36361 cttcgagcgg agtttcaggg tcggggcggt gatggtagaa aatgtccacg tattcgaggc
    36421 ccatgcgttt gaggctttgg tcgaggctgg agacgaggta tttgcgcgag ccccaatctc
    36481 cgtagggacc gtcccacatg gtgtaacctg ccttggaaga gatgatcagt tcatcgcgaa
    36541 atggacgtag atccttggcg atgatttgac cgaaggtttc ttcagcagat ccagggggag
    36601 gaccataatt gttggccaga tcaaggtggg tgatgccgag gtcaaaagcg cgcaagacca
    36661 tggcgcggct gttggaaaat gtatccacgc cgccgaagtt gtgccacaag cccaatgaaa
    36721 cggctgggag tttaagtccg ctgcggcctg agcggttgta gcgcatggtt tgataacgag
    36781 attcgtttgg taggtagttc atagtttctc ctggtatgtc attgcgagga gccgttcttt
    36841 ggcgacgaag caatctccag ttgagttaga gattgcttcg gacaagaaca agaacgtcct
    36901 cgcaacgaca aaaatttttt tactgcttca cttttaatcc gtccacaccg tagtaacggg
    36961 cgccgcaatt gagggagaac tgacgccagc cttttccgtt ggaaccgaac atgtcgcggg
    37021 tgtgaatttc acccgtatca atggctggga cttcgatcaa caggttgcct tttgcttttt
    37081 cgctttcgat cagggcatcc cgctcatccc aaatttgggc gcgatagata aaaccgtaac
    37141 gttcaccgta aatgttgacg atgcttcggg ctgtgtatgc atagcctgca agcatgacca
    37201 caacagatgc cagcacaagc cattgacctt tgatattttg ggcagcccac atgcctgcga
    37261 tccacgccat gacggcgatt ccaagcagca gggtgaagcg cgagagcgac tgtccacgcg
    37321 ggtcgggggt ggcactgtag aaatatgcgc tcggcgcttg aatggcggtg atgagcaggt
    37381 aagtgatgag ggtggtgaat gccaggagaa ccgcagcccg ttttacggtt aaggttgaat
    37441 cgtctgcttt tgtggtgata atgccgaagc cgaggaatat tccaatgaag acggcatgtg
    37501 gcaggggcag gtctttcaaa gagaaaacga tgaagtcaat ggcgtgtttc agcgaaacga
    37561 aagggacgct gaataggctg ctgcgtttga cattcatgtt tgcgacacgc gcgttggagg
    37621 gggagatgat cagggcgatc atgccgagca ataagaacag ccatgcgatg aaaagggtct
    37681 ggaaggattg ctctgcccaa tgttgctttt gctttttcgc gtaccatgcg gcgagtagga
    37741 atagcgttgt gccgctgaag agaaatacgc agctaatttc gccaagccca gccgcgatga
    37801 atgcaagcgg cgcaacgatg taatttaccc agcgggcggg cgtttcgcga tttatttggg
    37861 aagtgacaaa gccaaggatc agcatccaga acacgatggc ggtgctgtag ggaagtacac
    37921 ccgagcgcca gtaaaggatt tgaaaacgct gcggtgaaag atagaggttg taaaaaagca
    37981 gcagtgacga tgcaaacagg atggctgcga agggcagagg tttgatgagt ttggaaacat
    38041 tccagcctgt ccacacgagg cccaccagcc agagtgagat ggtgagtgtg gcaacaagtt
    38101 gattgccgaa catgccgagt gtgttttcgg taagcgcgga gaagaaggtg agcgagtagc
    38161 ggttggagga atatcccgtg tcttcggtgt cgaaatattg cagcgtggct ggaatggtgc
    38221 caagctgacg ggcatcggcg ctgtaacacc agtcatcggc ccagtagcgg ttgtagaagc
    38281 cgagataggc cagcaaggcg aggcctgaaa gaacggtgat gttgccaagg atgacgataa
    38341 gggtggtgag ttttttctgc atgtgttaat ccgtgataaa gatttggcgg tttttgagaa
    38401 tatcaacact ttaccacgga gacacagagt cacggagtct tttttacctc tgtgtcttgg
    38461 tgtctccgtg gttcaaaaat tatcattcaa aatgataaga gattttatat cacaccttgt
    38521 tcgatcagac tttcggccat gctcaatata ccttcttctg cagagcgcgg agtccatttg
    38581 aggatgcgtt ttgcgttttc gttcgagagt tcgtgatccc aatcgaggct ttcggtgacg
    38641 cgggcaacct ttttatcgaa caaagcgatc aaacgcacaa ggatgctcgg gaattccagc
    38701 ttgttgattt tatatcttcc cgcatatttg ccgtgcagga gttctgcgat ctctttcagc
    38761 caaagtgaac ggtctgctgc aaccaggatc ctttgacctg ctgcttcggg cgtttgcatg
    38821 gcaagaatca atgcggaggc cacatcgcgc acgtccactg agcccatttt gatgcgcccc
    38881 acgccaggta cctgccccaa catgatcgtg cggaccaatt caatggaagt gcggaaatcc
    38941 ttgttcggca ctggccctag aatcaacgga gggttgatgg tggtgagctg cattttgttt
    39001 gtattttcgc tgccgtggat aaaatcccat gcggcgcgtt cggcgagggt tttacttttg
    39061 gcatacgcac cgatgtcttt cgtcagatca gaccaatcgg cttcgctgaa ggttttattc
    39121 tcgccattat gtcccgatga gatcgcggcc accgatgaca cctgtaccat acgcttcacg
    39181 cccgccttgt gagcggcacg caaaacacgt aatgtcccct gcacggcggg gacgatcaat
    39241 tcatcttcat gttttggctc gtacaatgga aatggagacg cgacatgcaa aacagtttcg
    39301 acgttttgca tcgcttcatc ccaacccgca tcctgactca ggtcagcgga cagaatctcc
    39361 agccgatcat ttgcctgtac gtagtttgag atggtcttgc gaaggtcagc ttccttgctg
    39421 agtgagcgga tcgtaccgcg gacgttatat ccctgctgaa gcagttgaat gacggtgtga
    39481 atggcgacaa atccgcttgc acctgtgacg agaatggggg aggagggtgt catatgtgtg
    39541 gacctttgtt gttcaaaaaa agccctgacc gttggtttgt cagggctttt tatttatgaa
    39601 taccttgttc tactttcgcg aactgcttcc agaatatctg accttgtgat gtttaatttt
    39661 atgctgcgaa cctcaaaagg tgattctctc gctggaataa cggggctgat gacaaagatt
    39721 tgtccatctt ttgtcttaaa cctgagttga ccttcctgca tggccttttt tagaaagaca
    39781 ggaaactgtt tttggatttc agatgaagaa tagatcacca tgtttatacc tctattactt
    39841 gtacgccttc tttttttgcg gtttcggcca gatctttgtc aagtgtaatt aacggaacac
    39901 tgtgttccag tgcgcattgg aggatgtaag catcgtaagc gtaaatatta tgttttcctg
    39961 ccagttgtat cgctgcctgt aaattgatct ctacagtttc cattgagatt tcctgataaa
    40021 tcttgatggc ctcaatggct tgctccaaat tcactcttgt tttcttcatc attgcagaaa
    40081 aggcatttcc aacctcccaa gttacagatg gaggaacaat aatggttgaa ccttttgtca
    40141 actcaacgag ttttgccttt tctggttcat cggcaatgac tgcaataatg gcggaggtgt
    40201 caactgcgat tttcatgatt gattttgatt atagcatggc ggtaactgaa gtccgcgctc
    40261 ccggggttag aaagattcgt acaaatccgt ttcgaagggc agggtccctt tgcgaacgga
    40321 ttcgatctgc tctgtggtga gagagttgcg ccatttatcg gcctgcttgc ggctgtcacg
    40381 ttggatgttg tagtaaccgc cgtccatgcc tgtggtgctt ttctcgatct catcagcaat
    40441 ggactggttc catggcaggc cgagttgggt gtacagtttc ttgaattcgg ctacgggatc
    40501 gcccgtcagt ttttcaaaat agacccacag ccactttggg ttttgacggt gcagttcctt
    40561 gatcgtcttg ttggtgcggc cccaatcctg gctgatctgt tcgagggctt cgagagaaat
    40621 ttcttcaatg ccctggtcat gctggagctg gcgcaggatg ctttcacttc tgcctgcggg
    40681 gtggcggcaa atgtagacga tgttggcgtt ggtcagcgct tcgctggatt cgccgatgac
    40741 ttccacaaaa ctgatgggtt ttatgacgat ggccttcgaa aagatcggcg ccttgagcac
    40801 ggcaaaaagc gaaatgcagg ccagtgtgtg gaccttgagc tcggtcggca cttcagagtg
    40861 gacaaagtcc cagaagtttt gctgggtgta gtgacggtcg gggccgaagt gctgtttcat
    40921 caattcggtc tcgggttcgt gcatgtagat ggaggacttg ccaagcgaga ggaattttcc
    40981 cagcatgctg gttccgctgc gactcatgcc aaagatgagg atgacctttt ttctgcgcaa
    41041 tgcgatcatg actcgcgtga ggaatgcgat cagtttcctg tatcgcagag tggcgaggat
    41101 gaaggaatag gaacgcagtt gcccaggttt gttcatgtgg gttaaataaa gaaaagcaac
    41161 agagcaacca aagtacctgc tgctccacag gcgaagttga cacggtcatt atccagccat
    41221 tgccatccgc gtgtgtggac ggtgggtgta ccgcaggtat gcagggggtg tttctcggtc
    41281 tcttttttat cggtggggca gtagtacatg gcttgcacag ttccgccgag gaaggagtcg
    41341 aagagcgaac cagccaaccc ggagagagtg atcaatggga acaggaacca gttgtcggtt
    41401 aggagcgaag cgaggaatgc aattaaggcg gatcctgtta atgaggcgag cgtgccgatt
    41461 aatgagattc cgcccgaggt gcctttttcc acgattttgc gtagatcggt gatcatgcgc
    41521 ggcggattcg gattcaatac gccgagttcg gtggcccatg tatcggcatt gaccgcggca
    41581 agcgcggcgg cgaatccgac ccagggcagg cttgattctg ggaaaaagaa atgaagcgcc
    41641 gcgaagaagg tggctacgcc gccgttgcca aagacctgcc ctgcatctcg ttcatggcct
    41701 ttggaaaatt tttcgtcgag cccttgtttg cgttttttga aggcgcggct cagggctgat
    41761 gacgtgatga aaaaagtaag cagtagaacc gcccattccc agccgccgat gccaaaaatg
    41821 atcgtgccgg tgaatgctgc ggcaatggct ccgctttggt ttaagctgcg ggctttgtag
    41881 gcgaggtaag caatgaggat tgcgagaagg aagccgagga gaagttgcat gggtttaacg
    41941 ttggaacgtt ggaacgttca aacgttccaa cgttttttca aacaggagtt gtgatgatga
    42001 aaagcacggc cagcaaaaag agcaggcttg aaaatgtgct ggagccccac acgataaaag
    42061 caaggatgcg ttcacggtcc cagcgataga aatataaacc tgccacccaa cccatcactg
    42121 caagcaaaag actggcaaca gggaagatga tgagctgtgc cgaagggacg gtttcaagcg
    42181 cgctgccgac gaattgcggg ccgagcgcca cacgtggtgt ggatgggatg atgagactgg
    42241 cccagatgaa caaccccaga ttcaaaaaca aggcggtgag ccagaggtaa cgggcaaggc
    42301 cgctttccca agcctgcgtg acgacgaagg atggatacac cgatttcgct tcaacaggtg
    42361 cgaggcttcc caattcggtt gcgcgggcaa aagtttgcat gagcagggcg gggtcttcgg
    42421 gcgaaatgac gaagacacgt ttggctgtgg caaccagcaa aagttttttc gcatctgagg
    42481 cgaggaattc caccacgccg aggtcggggt gacggcgcat tccaagcaaa ccgccaggca
    42541 gccgaaccga aggcaggttg agcgggcggg tgaggtcatc ggcggggcgc atccactcaa
    42601 tgtcgctgag cggaatatct tccacgcgca aaccccaatt gatggcgagg ctgtcgcgat
    42661 ccatgtgata gtcggcgcgc cagagggaat agccgcgata tataaaaaac gggatcggtg
    42721 caaaggcgaa cagcgaaacc aacagcgcga ccaaaaattg cgggccgact tcagcgcgtg
    42781 aaaggttgat aaacccaatg aaggcgatca ccatcaatac aatgatgatg ctgccatgca
    42841 cgatcaaacc gcggcgtttg ggtgggggga aatgtccgat gttcattggg aaaggatgaa
    42901 agatgaagga tgaagttatg ccttcatgac ctcttcacga atgatgttcg aaacaaaatc
    42961 cacttgttct tcggtcatca caccgctgaa gggaatcgcc aaaccgcgct ggccgagatc
    43021 ttcggtcacg gggaaatcgc cttccttgta gccaaacttt tccaccatgt acggttgtaa
    43081 atggatcggc aaaaagtatg gccgcacggg aattccacgc gcatcaagtt tctttgccat
    43141 ttcgtcgcgg ttgattcgtt tgtcaaagcg gatgacgtac acaaaccacg acatgcgcgt
    43201 cgtgaaggat tcgatgtatg gcgtttcaac gccagggatt tcagaaagcc gcgcttcata
    43261 ccaggccgca acctgattcc gcttcgtgag aatttcatca agccgggtca tttgcgaagc
    43321 ccccatcgcc gcagacattt catcaatgcg atagttgtag ccgaggtggg tgtgttgcag
    43381 ccaggtatca ccgggggcgc gtccctgatt gcgcagggca cgcataaaat gcgcagactg
    43441 gtcgtcactg gtgatgatga ccccgccctc gcctgtggtg atctgtttgt tgggatagaa
    43501 cgcgaacacg ccgaaatcac cgagcgtgcc agcctgtttg cctttgtagg tggcgcccag
    43561 ggcttcgcat gaatcttcga tcactttgag cccgtgctct ttcgccacgg cattgatcgc
    43621 atccatgtcg gcaggttgac cgaacacatc cacaggcagg attgctttca attttccgct
    43681 tttgtccgcg ccttttctcg gcaggaattt ttcaatattc ttcgctgcat cagccaccaa
    43741 ttcggggttg atgtttccag tcttcgggtc aatatctaca aaaacgggaa ttgcattttc
    43801 aaaaagcaaa gcattcgatg aagccacgaa cgaaaacggc gtggtgatga ccagatcccc
    43861 tgcgccgatc cctgccgcac gaacacacag gtgcaggccc gccgtgccag agttgacaga
    43921 gatggcgtgc tttgcgcccg tcaggtcaca gaaggtcttt tcaaaggcgg gagtccattt
    43981 gcccatgctc aaattgggcg tgttgatcac ttcgatgaca gcctgacggt cggcatcggt
    44041 cagatcgggg gatgacatgg ggatttt
//
LOCUS       DAOQJC010000015        43232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_377_length_43232_cov_10.032471, whole genome shotgun sequence.
ACCESSION   DAOQJC010000015 DAOQJC010000000
VERSION     DAOQJC010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 43232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43232
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_377_length_43232_cov_10.032471"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2313)
                     /locus_tag="PLE39_03680"
     CDS             complement(<1..2313)
                     /locus_tag="PLE39_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67453.1"
                     /translation="MNQTNTQLKRVSRPRWSIRFTSLRTRLLVTSILLVLLPVSITGA
                     VATYISSQGLTADVFDQLESVVTLKESQVHDWLASLQTNLDMVFVNVDSLKRVSELVL
                     NREPDEQQKDLIRAELTNNSQRSGYFTEIFVMDKDGQVIVSTESNQEGKILTTQSFFH
                     EGIKGRYITPPSYELSLSNYTIVISQPLVSNTGKTIGVVAGRVNLNTLDEIMQQRTGL
                     GETGETYMVSSNFAVLTKLRFGETVLGQTYIHTDGVTNAIKSKANGSGSYPDYRNTAV
                     AGVYHWVPELQVAIIAEQDESEALSAASRVFQVTLGLIVITVLAAGFAAFLTTQSITA
                     PVTKLATAAENIAKGNLDQHVDIIQQDEIGVLANSFNSMTSRLRELITTLEQRVADRT
                     KALATSSEVSRRLSTILDQRQLVTEVVEQVKNSFNYYHAHIYMYDEEHGELIMAGGTG
                     EAGATMLANGHKLSKGQGLVGRAAESNEPILVNDTSHDPNWLPNPLLPETKSEVAMPI
                     SIGNKVLGVLDIQHNIVDGLKREDVDSLQSIANQVAIALQNIGSVETMTKRAKELASV
                     AEISTVSSREADINRMLENVVYLTQRRFGLYHAHVFLYDRNANHLQIIACGWKEGDEH
                     EGTHGTTIIPLLQEQSLVARAARTKQAVIINDVHNEPGWLPNPLLPDTASELAVPLLV
                     GDQLIGVLDVQSDRLNAFTEEDANIQTTLASQIAVTVQNARTLIQSKRQAERETLVNI
                     ITQKIQNATTVEDALQVAARELGHAMGMKST"
     gene            complement(2427..3437)
                     /locus_tag="PLE39_03685"
     CDS             complement(2427..3437)
                     /locus_tag="PLE39_03685"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE67454.1"
                     /translation="MKTLNRFSKLAVFTTWITILSLALSGCGNMNFSAPDKVTVQLSW
                     FPSVEYAGFYAAIENGYFKDENIEVTLIPGGPEVNPLAEVESGKSQFGITTGDSLIIA
                     RTNNQNYVAVATIFRQNPLVVMTLDSSSINKPEDLAGKKVGVIAPDLSTTYDIQFLAL
                     LRTLNIDQNSMTFEAIDDYNGASELITGGMDAMSGSFATNEPIQATMAGNDVSLIYYK
                     DYGVDVYANVLFTSEEFLQNNSDLITRMVRATMKGYQFSIENPDKAAEFALKYDPTLD
                     LAFQVNVMTAQIPIIDTGDAPIGAMDGDVWETTQQILLDFNLITEPIELNTVYTNQFV
                     GQ"
     gene            complement(3477..5816)
                     /locus_tag="PLE39_03690"
     CDS             complement(3477..5816)
                     /locus_tag="PLE39_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE67455.1"
                     /translation="MIPPETTPTSTNPVRILVVDDHPNTATTLARALSQLGTGVSVIS
                     ASSGHDALEKVKTNGVDILFTDMIMPEMTGLELIEKMQNHPAGRPSYTYLVTAYDVPG
                     LKVTAHRLKVNEVIVKPVRPERICQIATNAIEEMKHSTHPIKQSSVLRKKFKILVADD
                     RPDNVTLLTRYLEYEGYEHIAASDGLDTLDKVRAELPDLVLLDVNMPHKDGFAVLEEI
                     RSDPSVQHIPVIILTAARLDPSDVQSGLNLGADDYVTKPFDRHELMARIRTKLRVKET
                     EDIIRRRNRELNLLPEIGKELSARTDIKDLANILIKRTVETLGAIQGNMIIFGTNGGP
                     AQNYQVPAASPDSANSMIAIPKNIIKHVEESHQGLIIADARNNSLWQLDNQDSNIHSA
                     VISPLFGRMELLGLLVLTHEQVNYFNTDHLLLLQAITSQAAIAIENARLYSIAMQEQN
                     RLSAVLKHAAEAILVFDAQGKLSLLNPEGEKLFTDFSTNLNQPLPHDHGYDVFIHMLD
                     DARTTHGAKSGDITWPDQRTFNALITPIEDGGQVAILHDVTRFKDLEQVKNEFIATAS
                     HDLKNPITTIAGFSTLLEHAGPLNQQQKDFVTRIQTATHTMSELVQNMMSLAQMDLDA
                     TQKHEVVDMDKVMTEVVNEFIPQANIKNQRFSYTPLEQPVTISGDPLQLRQLLRNLLG
                     NAIKYTPTDGEITFKTQASDSQLLIEVKDTGCGIPAADLPFIFDRFYRVRSGHNIEVE
                     GNGLGLAIVKSIVEHHHGQVNVESEVDKGTSFTLSLPLS"
     gene            complement(5987..6778)
                     /locus_tag="PLE39_03695"
     CDS             complement(5987..6778)
                     /locus_tag="PLE39_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE67456.1"
                     /translation="MSNGHILIIDDEATLRQSLARILQQAGFEATTAENADQGLAFIE
                     TTLFDLVFMDLRMPGIAGLDALKLIHANYPTLPVVLFTAQPDLNSAVEALRSGATDYL
                     LKPLKPQIVIENAQKIIASRQKEKRKLEIMQQIENLQAELKGLNNGDTGPLPATRVDA
                     NRYIKRGTLVLDLHKRCLNINEHAVNLPPTSFEYLLVLARHSPDVVSYQTLVTEAQGY
                     QADLHEAQELSKWHIHQLRQAIEPDIHSPTYLINIRGTGYRLVAD"
     gene            complement(6775..9366)
                     /locus_tag="PLE39_03700"
     CDS             complement(6775..9366)
                     /locus_tag="PLE39_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67457.1"
                     /translation="MESSQAKWLFTGLSAVIYLIGSILLFNYFGVAITTLSGLPVVVS
                     AYFFGIPGGIIASLLTILVNTVLLVNVQKIPWAEINGYDYGLGAILITLLGISVGWIK
                     YKYEEYRSILSQLRSRERFLELLNDMAHAITTAQNFESMANTLTNDITTLLDADSCYI
                     TRWDPVKEQVFPVATNNLKNTAFLTMEYPKGDANLTITALQENRIIVVEDPHHSTYVS
                     ATLIHLFPEKSFISIPLNYGDHKLGAVIVGFAEPHQFTEEELNRAEQAGNQIALAIWN
                     LQQDNELQRGLREMQSLADISLALSETERIGLSNVLHLIVKSAKDLIANSEQAVIHLL
                     NEKDQILVSEAVVGYTELEGGKRNIRLGEGVAGQAILSGETINIADVRTDPRFLRLGV
                     EPAYRSLLVTPVRSGEQKLGTISIQSSRPYAFTPSDLKLLSQLGTQAAIAIENANLLE
                     NTKQALKEANALYRVNKGLVASLDPHDLLQDTVELLQKNFGYHYVQIYVANPDTGNFI
                     MRAGSGKVGALLKAREHQLAPGEGIVGYTAETGTAFFTNNVDDVVSFVRHPLLPDTKS
                     ELAVPVKVGKQILGLLDIHQVPPKYLSQRDLQLVSAVADQLAVALQKADLYETLQISL
                     QQEKTIRNQLMQNERLTVMGRLLATVSHELNNPLQAIQNALFLLKEETNVSPQGKQDL
                     DIVLAESERMANMIARLRDTYRPTHAEDFQPTQLNDIVNDVYALVSTHLRHNQISFEF
                     TPDPAIPFIPALADQIRQVVLNLLMNASEAMQNGGRIKVCTEYQKDTGEVVMIMSDNG
                     PGIPPKILPTIFEAFVTDKQSGTGLGLTITYDIIMKHRGRITAENNHDQGATFRVWLP
                     VTSGEIL"
     gene            complement(9558..11084)
                     /gene="rny"
                     /locus_tag="PLE39_03705"
     CDS             complement(9558..11084)
                     /gene="rny"
                     /locus_tag="PLE39_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNE67458.1"
                     /translation="MSPLVLIIDLIVLIVGVSAGYFFHRYQAEQAAKARQEKADDILK
                     AANSQARLIESGARENAAKITKAAEAEVKERRIELNKETERLDKRRSELDSRFDKMEQ
                     REQTLNKRQSQVDKRANEIDKLYDDQMKKLEFIAQMTQDEARKELFALVEKEARGDMA
                     RIIRQIEAEAREEGEKRARKLIADAIQRVASEHVAEVTRAVVTLPSEEMKGRIVGRNG
                     RNIKAFEQAAGVDVIVDDTPDSVTISCFDSVRREIGRRALSKLILDGRIHPAHIEKIV
                     EDETKAVEKIINEAGEQAAYEANVTGLHTEVLRMMGRLKFRTSYGQNQLAHAVEVSKL
                     AGILAAELGANIELAKQGGFLHDIGKAMDHNQDGTHAGLGAEYCKRYGVHPTVVNAIA
                     AHHHEVDQETVEAVIAEAADAISGARPGARREDLEAYIKRIRTLEEMSMSFEGVQQAF
                     AIQAGREVRILVKPDTIDDLSSARLARDIAKKIEETMQYPGQIRVTVIRETRATEYAK
                     "
     gene            complement(11081..11746)
                     /locus_tag="PLE39_03710"
     CDS             complement(11081..11746)
                     /locus_tag="PLE39_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HNE67459.1"
                     /translation="MKKITAISVQKKTPNRVNIHLDGEFAFGVARITAAWLKVGDELS
                     DEKIASLLSEDSRERAYQQAMLFLSYRARSEKEIRQNLHKHEYSEDVVETTIERLRDA
                     GLVNDDEFAQAWVENRSTFRPRSRRALAMELRQKGLNDETVQTAVSAVDEETLAYEAA
                     QKRLGRLKGLEWNDFRKKLSEFLARRGFPYSIIAPVVTRAWSETHPTGEQKIFDDENE
                     EIT"
     gene            12247..14214
                     /locus_tag="PLE39_03715"
     CDS             12247..14214
                     /locus_tag="PLE39_03715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE67460.1"
                     /translation="MTEQQQEATGGDIGRIRRFIAFLGILFILVSQFLIFSQPVDHTL
                     VFPPYTWLAIVGVLVLALGLFIRPTAFFQRISDWFVFQERTFWVLAAFLMSALVTMAT
                     ANLMLFARINYIPVLTIWLLSAWAYIYAFSNFSISGSAIFDWFKKNRNELLAVLAVTL
                     LAAGLRFYRLGGIPRILDGDEGLIGLAAQTTTSGLLANPFSLWENFGAMYLHLINTAI
                     GFFGVNAFALRLWPAIGGTLAIPSLYLFVRWIGGRRIALITALIIAFSHSHIHFSRIV
                     SVAYIHGTWLAPLELYLMLSGLEKRQSWRAALGGVLIAIHFSIYLTAQVIIALAFVFM
                     VLAFLFYRPWFKERFAQAMAFWGGFLIIILPSAFYIYRNPNEFFNRLAQNGTFQSGWL
                     ELTMQSTGQSAAEILFGRFVHAFLSLTYYPAIDFYGSSSPMMSMISSTMFLVGLLVAL
                     WRIRNPAYLLLNGYFWGATFSVGLFAIPPNSDSYRMLMALPSALTMAAMGLDQILELL
                     GLGWNKARTSYTLSASAILVSLLIFNLWTYYGDFAGKCRFGGDLVGRFASYLGSYAHS
                     VDNELPIYLLSNGIYFYGSHASTDFLSESRAITNFPDPIDTLQPVSGETIIANPERIP
                     ELEAWARLNPGGELHYRYDCSNTILLAYRVP"
     gene            14222..14956
                     /locus_tag="PLE39_03720"
     CDS             14222..14956
                     /locus_tag="PLE39_03720"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE67461.1"
                     /translation="MDPREYELMYQVEDRHWWYRGMETITRALLERWAPARPQRILDA
                     GCGTGAAMTTYLTDYGKVTGIDLYAEALKFCRERNAPRLARASVLNLPFAPASFDMVT
                     SFDVLYERAVSDELTALKEFFRILTPNGCVLLRLPAYDWLRGQHDRNVHTNRRYTKKL
                     VRHLLEQSGFTVEHLSHANTFLFPLAVMKRMGERVFPPKDDQSDLAMNAGVFNDALKI
                     ILSAEAPLVSSIGLPYGLSVIAIGRK"
     gene            14965..15708
                     /locus_tag="PLE39_03725"
     CDS             14965..15708
                     /locus_tag="PLE39_03725"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE67462.1"
                     /translation="MKHERESQQLPSISAVFPAYNDGGTIASMVAAAWIALNQVTSDY
                     EIIVVNDGSSDYTATMLDEISVRYPELRVITHPYNQGYGAALRTGFSAASKDWVFYTD
                     GDSQYNPLELVNLVGALYEGVDVVNGYKLTRNDSWMRIVIGRAYHYLVKFLFGIHIRD
                     VDCDFRLIPRRILEEIELKSVSGAICLELVKKIEDAGYVFAEVPVNHYSRKYGVSQFF
                     VPWRIARTLYQLADLYWKLVIRKEHLIKR"
     gene            15714..16703
                     /locus_tag="PLE39_03730"
     CDS             15714..16703
                     /locus_tag="PLE39_03730"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE67463.1"
                     /translation="MDIAAAYFRQKVLITGGAGFIGSNLAHKLATLGADVTIVDSLDP
                     DSGANLFNLNGIEKTVRLIQADINDERQMAQAVRGQKFMFNLAGQTSHLGSMQDPFKD
                     LDVNSVGQLRLLEVCRQNNPEISIVFAGTRQVYGRPKYLPVDENHLLTPLDNNGVSKR
                     AGEMYHIVYNRVHGMATCVLRMTNVYGPRMRIKDDLLTFIGWWFHQLLMGENIQIFGD
                     GRQVRDINYVDDVIDAMLLCAAHPSANGKIYNLGGAPINLLDLARLMIQVNGKGGYTL
                     VPFPENRKRIDIGDYYGDYSLIQKDVGWRPQVELETGISNLLAFYRMYKEHYL"
     gene            16709..17815
                     /locus_tag="PLE39_03735"
     CDS             16709..17815
                     /locus_tag="PLE39_03735"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNE67464.1"
                     /translation="MEEIPVIDLKKQYQSIRPELDAVFARVMEKGTFILGSEVSAFER
                     EFAEYCGAAHAIGVASGTDALQLALMACEIGENDEVAVPAHTAVATVSAVEAGGAHPL
                     LVDIDSTNYTLDPNLLHRILMPHTRAVIPVHLYGCPVDMEPVLQFARENHLFVIEDCS
                     QAHGASYRGRKVGTWGDIAAFSFYPTKNLGAFGDGGMVVTNNDALAEKVRLLRQYGWG
                     EHYISSIRGINSRLDELQAGILRVKLRHLDGWNARRRQLAELYHHLLSGTDLVLPAQN
                     AGHVYHQFVIRHAQRDSLREYLKENGISTLIHYPVPVHFQPAYADLGYIAGSLPFTER
                     ASREVLSLPLYPELTEEKVVRICQTILDFFTHSM"
     gene            17997..20264
                     /locus_tag="PLE39_03740"
     CDS             17997..20264
                     /locus_tag="PLE39_03740"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNE67465.1"
                     /translation="MAKITREDALEYHRLKGKPGKVAIVPTKPMDTQRDLSLAYSPGV
                     AEPVLEIEKNPDDAYEYTSKGNLVAVISNGTAILGLGDRGALAGKPVMEGKGVLFKKF
                     ADIDVFDIELNSHDPDEIIKVVAAISPTFGGINLEDIKAPECFYIEEELKKMLNIPVF
                     HDDQHGTAIISSAGLANALEIVGKRHDEIRLVISGAGASAISCAELAISWGVKRENIM
                     LCDSKGVVYKGRKEGMNKYKERFLVETDARTLTDALRGADVFYGLSVANVMTPEMVKL
                     MAKDPIIFAMANPDPEIKPELAREARKDVIIATGRSDYANQVNNVLGFPFIFRGALDV
                     RAKQINEEMKFAASKALAALAKEDVPDSVIRAYGGESIKFGREYIIPKPLDPRVLLWE
                     APAVAEMAMKTGMARKTIDIEEYKEQLAFRQGKGERIRYFFQNKARTSGGKKRVAFAE
                     GEEQKVIRAAYQIKEEGIATPVLIGNAGVIDEQLKVLGLDYKPVIVDPNKFEKLEAYA
                     KSYYEMRQRKGVMLSDAHNKVRDPNILGPLMVKMGDADAFVSGLTFDYPEVIRPALQI
                     HHTAPGATRAAGVYIMIVDDKVFLFTDATVNIDPTAEDLAEIASLAADFAVKLEIDPH
                     VAFLSFSNFGSTPHPLSDKVRKAVELTKKRRPDLRVDGEMQADTAVVPEISEERYPFS
                     AVKDANVLVFPSLESANIAYKLLSRLGNAKAIGPILLGMGAPIHVLQTGDDVNAIVQM
                     AAVAVMDVMGREANG"
     gene            complement(20376..20792)
                     /locus_tag="PLE39_03745"
     CDS             complement(20376..20792)
                     /locus_tag="PLE39_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67466.1"
                     /translation="MNSNDIFVIEWQDPRTKTVLSVKYLKPWQWSDVVNALKEAEHYF
                     KSIDHKAIIIHDQSKFPPEQSTIGAVKDLWGKLPLPPANLRACFVVNQSKNYMLEVAF
                     DIVEKAFFRRTVTHFVYTMGEVEKMLEAFDLNSRNR"
     gene            complement(20896..21303)
                     /locus_tag="PLE39_03750"
     CDS             complement(20896..21303)
                     /locus_tag="PLE39_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE67467.1"
                     /translation="MKNGPIIVYVEDDAPSIVVMKAIMNHVLGIVDNLIVIENTSDFL
                     EKVREIGSIPDIFLLDIHIQPYDGFELLKMIRSDNRLRYCKVVALTASVMNEEVEKLR
                     ESGFDGAISKPLDRYAFPKLIEKILDDQSVWHI"
     gene            complement(21306..23702)
                     /locus_tag="PLE39_03755"
     CDS             complement(21306..23702)
                     /locus_tag="PLE39_03755"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE67468.1"
                     /translation="MAKARSMKLNKLSVRELLLLGFMAILGFNLLGMGISLINFNSFQ
                     SALKATSQESSQIRELSLEIQTEFLLARQDESAFLANWRLLGFDNARKEYGEQIQFHL
                     DAARTHLNELQTLSINSKNKNFREIANSTVQLSALLGDYENSFNATLGKIQTRIGANG
                     LEKSLASGIEEMQFETRGLDNQFFFDTLIQIQKNEQGYFSTYRQEYIDYVRINTIELV
                     DLTQATEEGGLETLNAEQLSTNLLEHYDTFTKIVALDQDIIKNTTIFRDITIDINLIT
                     KTIGEVSRNGLILANQELDSTYRTTLLIQLTAGSLSLLSVIGVFIILARQTLRPLNQL
                     TLAAERLGQGDFEKNLDIHGLKEFSTLSQTFNSMSAQLSDLIESLEQHVAERTRDLQM
                     ATDVSRQITQELKLEKLLPNLVKQTREGFDLYSTSIFLYQPETDELQLEADDVKENGV
                     KTEPKSFHINTRPSLTAEVARTRKTEVLNNIDQSSFVPTLRLPSIKSHAAFPMIVGDD
                     LVGVLDLQAETIGRFKDNDIRIFSTLAEQIAIAVRNAQLYRNQEKVTEELKRTDIMKS
                     QFLASMSHELRTPMNAIINLIDLVASEMVGPINTEQQAFLNQSLRSSHHLLHLINDVL
                     DISKIQAGQLTLNLEQDVNIYEEIDTALKMSTPLIKESVEVIQDIDSDIPLIEADRRR
                     IRQVLLNLLSNAAKFTDKGSITLILKKRADYLLFAVADTGPGIPITAQTIIFEPFTQT
                     EDGRKRVEGTGLGLPISKNLVEAHGGKLWVESQPNEGSVFYFSLPYPQIPTYPILSQR
                     "
     gene            complement(23723..24817)
                     /locus_tag="PLE39_03760"
     CDS             complement(23723..24817)
                     /locus_tag="PLE39_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNE67469.1"
                     /translation="MSTKILKPVLCLSILTLMALLSACQGWSQQQTPEAEVSPEEINF
                     YNWDNYMDPNILSNFEKEYNIKVNYGTYTSSRDLRKRLKENPEAFDLFIDSDYAVSIL
                     QWDGLLAPLNKANIPNMKNLDPLFVSPQYDPDNRYCVPYQWGTTAIAYNRKATGRDLD
                     SWGDLFDPQFKGRVSFVESNRTAVGGVLLYLGFSPNTTNESEIYQARDYLIEHIDQFY
                     TLRGDDGQDLLVNGIVDVAVEYSGDVMQIAAENPDIKYVIPKEGAVLWTDNMCISVAS
                     PHKEQTEKLINYMMDPHIGAQLSNYIRYASPNQAALPFIEQSDLTNPIIYPPTNIRYR
                     LFYVVDVGQEANNIYDAAFEMILAAHDALK"
     gene            complement(24814..>25021)
                     /locus_tag="PLE39_03765"
     CDS             complement(24814..>25021)
                     /locus_tag="PLE39_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE67470.1"
                     /translation="TAQTIIFEPFVQTEDGRKRVEGTGLGLPISKKLVEAHGGRLWVE
                     SQLGEGTAFYFTLPYLMEESKSQI"
     assembly_gap    25022..25121
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25122..26792)
                     /locus_tag="PLE39_03770"
     CDS             complement(<25122..26792)
                     /locus_tag="PLE39_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNE67471.1"
                     /translation="MNASSLPTLPANSIGYQYLKWVSLLMVGFTPFVTILPIYIYSQS
                     PSWQLLCFFTAGMVYGVGGWLSYRQAANNNHYIALRVITTFYWFAPGLGCFFLSEMPL
                     LMLTAAIYHIILFITLYPERLPFRNILIHLGIYLSLCFTAIFLDFSVAIPKWGFWIIG
                     GTVIASDIAYSIPFFLNFKQIQLAGKILVVQIQLTLIGFTAFAFIANIPEELHMILLT
                     IEGVIWLTTVWVLRYLTTPLIQLTENAEMIKQGDFSRRTKIEMNDEVGQLGIAFNQMV
                     EQLYNTLNGLESIVASRTRDLNVASQVSRDITRELNLENLLPNLVEKTRVAFNLYAVS
                     LFLFNPQTQELLFEAGSGASGEHMKGQAKAFHISAQPSLVSKSAREQKAEIVNDTSRS
                     DSHFFNPLLPNTRAEAVFPMVVGEELVGVLDLQSEYPNRFAESDIQIFSTLAEQIAIA
                     IRNAQLYRNQEKVAEELKRTDLMKSQFLASMSHELRTPMNAIINLVDMVASEMIGAIN
                     EEQKTFLNQSMQSSRHLLHLINDVLDISKIQAGQLTLYIEQDVNIYEEI"
     gene            complement(26831..27253)
                     /locus_tag="PLE39_03775"
     CDS             complement(26831..27253)
                     /locus_tag="PLE39_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE67472.1"
                     /translation="MENTNLPLLNTRIFVVEDDVLNLSVISRPLSVSGASVFYNYNSI
                     GISLHIEQHLPIDIILLDIMLKRGVNGYDTFKEIKQNPKTAHIPVIAITSLDPETEIP
                     KAKAMGFSGFISKPIRVHGFAQHIADIIAGQKKWISSQ"
     gene            complement(27350..27982)
                     /locus_tag="PLE39_03780"
     CDS             complement(27350..27982)
                     /locus_tag="PLE39_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE67473.1"
                     /translation="MSPNTIRVVLVDDHDYIHRIVTTILNQTTDIKLVGHAKNGEEAI
                     WVCEKLHPDVVLMDVLMPGINGLEATKALQAKMPNSKILILTSLQDQDMLFEMLRSGA
                     AGYVLKTTLKDDLAETINMIHRGKMVFSPEAFKTTPSDWEQTGSSLGLTKRELDVLRA
                     MADGMNMPETAARLKISATTAKFHMENICGKLGVRTRSQALIIAVKLKLI"
     gene            28280..29524
                     /locus_tag="PLE39_03785"
     CDS             28280..29524
                     /locus_tag="PLE39_03785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE67474.1"
                     /translation="MTNLESIARKITSILFAQQSLASAGFIAAATLNSIVGKELSGHS
                     DWAGVPSATYLLAGAFAAFMWGYVYDMIGRRNGLTIGLGIGAVGSGIAFYAIAIHSFA
                     IFLGGMVLMGIANAAVQLGRFAAAEVNPPEHRGRAISNVVIGGTVGSVIGPFVAGPAG
                     DLIGSWGIDELAGAYLVSLALFVIAAIVVFIGLRPDPREIGKQVAEKYPDTIVRSRVV
                     RSMMQIFSQPAALVALTSMVLGQMVMVLVMVITSLHMRGHHHDLTDISLVISSHTFGM
                     YAFSIISGRLSDQWGRGPVIITGSLTLVIACIAATISPDVLPLGVALFLLGLGWNFCF
                     VGGSTLLADQLSPDERARTQGFNDLLVGLASAIGSLSSGFIFAALGYNPMALISAIFA
                     LIPLLLASFWTLKRARKPRAIA"
     gene            complement(29586..30296)
                     /locus_tag="PLE39_03790"
     CDS             complement(29586..30296)
                     /locus_tag="PLE39_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67475.1"
                     /translation="MSMEDILKVLINSRQQGNSAQEADPMTSLVGSLLGGGQSQGGIN
                     LSDGVDAGDVAGLVGSLLGAQQAQNTPSQPQTQGLGGMMGALEGIMGGGQQANDPIMA
                     MLQPFIEPLAKKVNISPEIAMVIVSFVAHKLLAHHPTSGRDSNTFDLDEMLGQMGAGQ
                     VDSSLLHNSGMVKEISKRTGLDEATAEKSLNAAFTLVGNRVGLGNASPKPKMPGNSQP
                     NPSGPKTGMNSKSKSGKK"
     gene            complement(30365..30667)
                     /locus_tag="PLE39_03795"
     CDS             complement(30365..30667)
                     /locus_tag="PLE39_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE67476.1"
                     /translation="MPVYTYRCEACGVQFERHQSFTDTPLKTCPECRKKALKKVITPT
                     KIIFKGSGFYATDHKSPSGESSSTSSTKKTSKKEKDSAPKESASTESKSPSGGDSE"
     gene            30867..31055
                     /locus_tag="PLE39_03800"
                     /pseudo
     CDS             30867..31055
                     /locus_tag="PLE39_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            31094..32080
                     /gene="trpS"
                     /locus_tag="PLE39_03805"
     CDS             31094..32080
                     /gene="trpS"
                     /locus_tag="PLE39_03805"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNE67477.1"
                     /translation="MAKKGRVFSGARPTGRQHLGNYLGAIKNYVAMQADYECVYCIVD
                     VHALTTVETTQELKQNTFEMALDWLAAGIRPEDSILFVQSHVPEVMELHTYLSMVTPL
                     GKLTDLPTFKEKVRQQPENINYGLVGYPVLMTADIVLYKTDVVPVGIDQAPHIEFARE
                     IVRSFNYRYKTKALVEPQVKHTEVLKVVGLDGKDKMGKSLNNHIELASTPEETVKRVR
                     EMVTDPARQRKTDPGNPDVCNVFTMHKLFSAQEEVDMINTECRKAGIGCVDCKLRFAG
                     NLNKHLEPFRAKRAELASNPNHIQEILNDGAKRARTIAQQTMAEVREAMQLP"
     gene            32160..32597
                     /gene="dtd"
                     /locus_tag="PLE39_03810"
     CDS             32160..32597
                     /gene="dtd"
                     /locus_tag="PLE39_03810"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNE67478.1"
                     /translation="MRLLIQRVSQASVTVEEQTISKIGKGLLILLGIGHGDGEEQAAF
                     LAEKTANLRIFEDDQGKTNLSVLDVKGEAIVVSQFTLYADARKGRRPSFIDAALPDVA
                     APLVERFVELLRSHGVPTQTGRFGAHMDVEIHNDGPVTIWLEK"
     gene            32594..33082
                     /locus_tag="PLE39_03815"
     CDS             32594..33082
                     /locus_tag="PLE39_03815"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HNE67479.1"
                     /translation="MTIKPNFFQKFVHRFLMMRWVSAFLAVSLHRLDAIVLKLSRGRH
                     TVTELVGLPIIQITTIGAKSGEPRTMPLVSLFDGEKIALIGSNFGRQHNPGWYYNLKA
                     NPQCVVQTHGREQKFIARQAVGEEREKYWQMAVSYYKGYELYKVRAAHRQIPVMVLEP
                     MK"
     gene            33256..33465
                     /locus_tag="PLE39_03820"
     CDS             33256..33465
                     /locus_tag="PLE39_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNE67480.1"
                     /translation="MSDRVIGTVKWFNGTKGFGFIEREGGPDVFVHFSAIRGEGFKNL
                     QEGQKVEFTIEKGPKGLQAADVVVL"
     gene            complement(33601..34368)
                     /locus_tag="PLE39_03825"
     CDS             complement(33601..34368)
                     /locus_tag="PLE39_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNE67481.1"
                     /translation="MKRDIYFSKPIINAAGSLGFSPDPRAGIPFDRFGAFITNPISLR
                     PRLPTPQPALIEFPGGFLLHTGLPNPGLPAVIKKHSARWNRADLPIIPHLMADRPEET
                     AQMVRMLEGIENVMAVELGFAPLLSNDILLLTLDMCLGEVPLIFSLPMEQVLSLGSKV
                     IEKGAVAISIACPRGALVASRDKNKLTTGRVYGPSIFPQALNTVRAAAMSGIPVIGAG
                     GVWTEKDAADMLSAGAMAVEMDASLWSPKEAGAAPQI"
     gene            34493..35650
                     /locus_tag="PLE39_03830"
     CDS             34493..35650
                     /locus_tag="PLE39_03830"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNE67482.1"
                     /translation="MQPLQGIRVLDLSRVLAGPYCTMVLGDLGAEVIKVEPPEGDETR
                     AWGPPFAEGESAYYLCVNRNKRDIVINLKTDEGKKVLRDLALQSDVLVENFRPGTLEK
                     FGLDFATLSQSNPKLIYCSITGFGQTGPMRDKPGYDFMIQALGGLMSITGEPEGEPMK
                     TGVAVVDLFAGQNAVIAILAALQARTLTGRGQQLDIALFDSQLGWLANVASNYLISGK
                     LPKRHGNAHPNIVPYQSFQASDGWFAIAVGNDRQFGRLCEVIGRLDLANDERFAKNSA
                     RVQNREELIGLLKSIFLMRPVSEWLSALDEAEIPCGPINTFEQVFSMPQVQEREMLVK
                     MDHPTIGELPLVGSPLKFSDTPVQYRLPPPLMGEHTEEVLRELRDRGMKGE"
     gene            35683..>35886
                     /locus_tag="PLE39_03835"
     CDS             35683..>35886
                     /locus_tag="PLE39_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67483.1"
                     /translation="MKWLIPFFLILLGVSIFCCGFMYDAVFAGIPYQDPTPELVAKYN
                     FHSEVASVIRWGGAGTCAFGVVVA"
     assembly_gap    35887..35986
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(35989..36573)
                     /locus_tag="PLE39_03840"
     CDS             complement(35989..36573)
                     /locus_tag="PLE39_03840"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE67484.1"
                     /translation="MKLSTPEFRAMQSLPRKLGQRFFEMPVFRHMGLDLQNKDVLEIG
                     CGSGYGADLLNQLNPKSYIGLDVMEEQIELARVKYPQFEFLLQDATDLSQFSTTSKDV
                     VIIFGVLHHIPDWRKAIDEIVRVLKPGGSLFLEEPRGVDIKAFDFLFRWGHPDTDFGL
                     KVLDEHLTKRGLTILRKQWSPLLTMYHIKKRNDP"
     gene            complement(36615..37925)
                     /locus_tag="PLE39_03845"
     CDS             complement(36615..37925)
                     /locus_tag="PLE39_03845"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE67485.1"
                     /translation="MSNKFFVAVIGAGPAGLFGARELATLGAHVAVFNRDVKAGGLAE
                     YGIYPDKHTMKEGLRKQFRQVLDLANIDYYGNVTIGANGDLTLDELRALGFDAVLVTA
                     GAQGTKSLGLPGENLEGVYHAKDVVFSYNKLPPFSQKKFHFGKRCAIIGAGNVMVDIA
                     RHLLHVQNVDEVTAIVRRGPAEVNFTKEEMKHLISYLDLDDFNREMERVRPAVEAVKQ
                     DLETGRQKILDSLAKADPKTAHGKFRFDFLASPTAMYGENGLLTKLEVEDNILTERDG
                     KTSAKGTGAKRTLDVDTVIFAIGDKVDESFGLPTEWNEFIKNNSPRFPVDNLTFESAV
                     EGIFVGGWSRKASEGLVGYARKDGTNAAKAVWQYLQTKQPANASAEVVAEKMKNLGKP
                     IILKEDIKRLEAVEAEEARKRGLEEFKFASNEEMLQAMGFMETA"
     gene            38056..38892
                     /locus_tag="PLE39_03850"
     CDS             38056..38892
                     /locus_tag="PLE39_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67486.1"
                     /translation="MAEDNKKDPAQKMRYLLNGGKPSPVARLPKKDGGVASFLPKLKK
                     DDAPTETPAAVEVDVAPNKDQPEAAHQTPPKPAPFQGALSSLKGIKLNIGPAFWTIAS
                     VISMTVNVVLLIVLLALLPNLTKLNLGSALDIGNYLLGGLYSNFEKMDRAHITTSIPV
                     ETTIPVKFDLQLNQQTNVVLSQDVTITNALVTVNTGGLNITRALTTIVLPQGTNLPVV
                     LNLVVPVDTTVPVVLNVPVDIPLSQTQLHEPFAGLQDVVRPFYCMLSPNALGIDGQPV
                     CK"
     gene            38960..40225
                     /locus_tag="PLE39_03855"
     CDS             38960..40225
                     /locus_tag="PLE39_03855"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNE67487.1"
                     /translation="MTNQITQVTLKNGMKVMLKEIHTAPIISSWIWYRVGSRDEPTGK
                     TGISHWTEHMQFKGTKKFPGNVLDKAISREGGRWNASTSRDSTRYFETMPAAKIDLAL
                     RLESDRMKNSLFDEKEVASERTVIMSEREGLENEPTFRLSEAVQQTAFHVHPYHHEII
                     GDMADLRTITRDDLYEHYRRYYVPNNSILTIAGDFQTKAMLKRIQELFESIPMGKTPP
                     RLNRPEPEPKGEVRLTVEGPGETTFIQVAYRFPNAAHPDYFPLQVLDSLLGGASGLSY
                     KTSRLYRALVDKEFAVDVSSWPEATIDPFLYRIIVTNHPKRKTDQTLAALDAEIQKLQ
                     EKRVSKDEITRAVKQARAVFAYGSENITNQAFWMGYAEIFSSYRWFTSHLSNLAKVTP
                     QDVQRVAQKYFQPANRVIGIYTPNGRRSK"
     gene            40222..41508
                     /locus_tag="PLE39_03860"
     CDS             40222..41508
                     /locus_tag="PLE39_03860"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNE67488.1"
                     /translation="MNKPVSVKKKSSLPGPEDIHREILSNGITVLARSNFNSPSISMA
                     GYLPAGAIFETDDKLGLADFVSASLMRGTQTKSFDAIYNELESVGAGLGFDSGVHNAS
                     FGGRSLVDDLPLMLKLLSESLRTPVFPKNEIEKLRAQILTGLAIREQDTSDMAAMAFD
                     RLLFDGHPYSRPSDGFVETIQSITQKDLVNFHRDHYGPRGMVIAIVGAIDPKKAVAAV
                     ERVLGGWQVPGQVVPPLLPPYKPIKKTVRHHHTLANKSQSDLVIGMIGPKRKDPEFYS
                     ASLGNSVLGQFGMMGRIGESVREKSGLAYHASSSLSAGVGPGTWEVNAGVSPKNLKKA
                     IDLIEEELRKFVKSGVTKRELEDSQANYIGRLPLSVESNGGIANALLNIERYNLGLDY
                     YQRYEGLVRGVTRDQVLATAQKYIDPNRLVISTAGP"
     gene            41521..41886
                     /gene="acpS"
                     /locus_tag="PLE39_03865"
     CDS             41521..41886
                     /gene="acpS"
                     /locus_tag="PLE39_03865"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNE67489.1"
                     /translation="MKLATGVDLIEVSRVREAIERHGERFLARIFTEIEQRECGGKFP
                     SLAARFAVKEATAKALGTGIGDVSWLDIEVRGDENRAPHLYLHGAGEELAQKLGLVTW
                     SVSISHTETQAIAFVVAIG"
     gene            complement(42249..43040)
                     /locus_tag="PLE39_03870"
     CDS             complement(42249..43040)
                     /locus_tag="PLE39_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67490.1"
                     /translation="MDNSNHFTLMINALTELAKEEQSGHTSRMPEIILPTLNSVLADI
                     TPLPEEALFLGIAEDGLPVLLNLDDPIPGPILIVGDKACGKTGLLQTIAQAAELLHSP
                     SDIQYCVVTSHAEEWNNFQDRESNMGVYTTQDNGAQELIQSLVEWAHQNKNDERTFLL
                     LIDDLEFLTKLDQETEQCLRWLLLRGTSRKVWPIVTLNASRAHHIETWLGFFRTRLFG
                     NIQNLQDAHLVANASNDILGTLATGSQFAMREGQNWLRFLAPALD"
BASE COUNT        10072 a         9840 c        11242 g        11878 t
ORIGIN      
        1 tgtggatttc atgcccatgg catgccccaa ttcacgtgct gcaacctgca aggcatcttc
       61 cacagtggtg gcgttttgaa ttttctgggt aatgatgttt accagggtct cacgttccgc
      121 ctgtcgcttg gattggatga gggtgcgggc gttctgcacg gtcactgcaa tctgagaggc
      181 aagcgtggtt tggatgttgg catcttcttc ggtgaaggcg ttgaggcggt ccgattgcac
      241 atccagtacg ccgatgagtt gatcgcccac cagcaatgga acagcgagct cggatgcggt
      301 atctgggagc agcgggttgg gcagccagcc cggttcattg tgaacgtcat tgatgatgac
      361 ggcttgtttt gtgcgggcgg cacgtgccac aagggattgt tcctgcaata atggaataat
      421 ggttgtgccg tgtgtgcctt catgttcatc gccttccttc caaccgcagg caatgatttg
      481 aaggtggttt gcgttcctgt cgtacaggaa gacgtgggca tggtacaacc caaagcgtcg
      541 ttgggtcaga tacaccacat tttccaacat acggttgata tccgcttcac gggatgagac
      601 ggtactgatc tcagccaccg aggcgagttc cttggcgcgt ttggtcatgg tttccaccga
      661 gccgatgttc tgcaaggcga tcgcaacctg gttggcaatg gactgcagtg agtcaacatc
      721 ttcacgtttc aggccatcta cgatgttgtg ctgaatatcc aaaacgccca acactttatt
      781 accgatggaa ataggcatgg ctacttctga ttttgtctct ggcaaaagcg gattgggcag
      841 ccagtttgga tcatggctgg tgtcatttac caggatcggt tcattgcttt cggcggcacg
      901 acctacaaga ccctgacctt tggaaagttt atgtccattg gccagcatgg ttgcgcccgc
      961 ctcacctgta ccacccgcca tgatcaattc gccatgttcc tcatcgtaca tgtaaatgtg
     1021 ggcatgatag taattgaagg agttttttac ctgctccaca acttctgtta ccaactgcct
     1081 ttgatcgaga attgtagaaa gacggcggct gacttcggaa gaggtggcca gcgcttttgt
     1141 acggtctgca acgcgttgtt caagagtcgt gatcaactcg cgtaaacggg atgtcatgga
     1201 gttaaaggag tttgccaaaa caccgatctc atcctgttgg ataatatcca catgttgatc
     1261 cagattccct ttggcgatgt tttcagcggc ggtggccagc tttgttaccg gggccgtaat
     1321 gctttgagtg gtgaggaagg ccgcaaaacc tgcagcaaga actgtgatca cgatcaaccc
     1381 aagggtgact tggaacaccc tgctggcagc gctcaacgct tcgctttcat cctgttcagc
     1441 gatgattgcc acttgaagct caggtaccca gtgatataca cctgccactg ccgtattccg
     1501 gtagtctgga taactgcccg agccattggc cttgcttttg atcgcgtttg taactccatc
     1561 ggtatgtatg taggtttgac ccagaacggt ctcaccaaaa cgcaatttgg tcagcacagc
     1621 gaaattggaa ctgaccatat aggtttcacc agtttctcca aggcctgtgc gttgctgcat
     1681 gatttcatca agcgtattta agttgacccg ccctgccact acaccaatgg tttttccagt
     1741 attgctgaca agcggttggg atatgacgat agtgtagttg gagagcgaga gttcataaga
     1801 aggcggtgtg atatatcgcc cctttatacc ctcatgaaag aaggattgtg tcgtcaggat
     1861 ctttccttcc tgattgcttt cagtggaaac aatgacctgt ccatccttat ccatcacaaa
     1921 gatttcagtg aaataccctg aacgctggct attgtttgtc agctctgctc ttattagatc
     1981 tttctgctgt tcatcgggct ctcgattaag cacaagttcc gaaacacgtt ttagcgaatc
     2041 cacattgaca aaaaccatgt caaggttggt ttgcagagat gccagccaat catgaacctg
     2101 agactccttc agagtgacaa ccgattccaa ttggtcgaaa acgtctgctg tcagaccctg
     2161 agaactgatg taggtagcca cagcaccggt aatggatacc gggagcagca ccagcaggat
     2221 ggatgtgaca agcagacgcg tacgtagcga tgtgaatcgg atcgaccaac gcggacggct
     2281 gacgcgtttt aattgagtgt tggtttgatt catgagcgcc ctccggcaag tgagcaaaag
     2341 acagagctat atattgtgtt gacgaatttt aagcctatca tacggtatat cttcacgagc
     2401 aagccttgca ttgtacgtac aagttcttac tgaccgacaa actgatttgt gtacactgtg
     2461 tttagttcga tgggttcggt gatcaggttg aagtccagca ggatttgctg ggtggtttcc
     2521 cagacatcac catccattgc gccgatgggg gcgtcacccg tatcaataat ggggatttgg
     2581 gcggtcatca cattgacttg aaatgcgaga tccagggtcg ggtcatattt caaggcgaat
     2641 tcagcggctt tgtcggggtt ttcaatggag aattgataac ctttcatggt ggcccgcacc
     2701 attctggtga tcaggtcgga attgttttga agaaattcct cagacgtgaa aagtacgttt
     2761 gcgtacacat ccaccccgta atccttgtaa tagatcaggc tgacatcatt cccggccatc
     2821 gtggcctgaa tgggttcatt ggtggcgaat gagccgctca tggcatccat gccgcctgtg
     2881 atgagttcgc tggcaccgtt gtaatcgtca atggcttcaa aggtcatact gttttgatca
     2941 atgttcaggg tgcgcaataa agccaggaat tgaatgtcat aggttgtgct taaatccggg
     3001 gcaatgacgc ctactttttt gccagccagg tcttcaggct tgttgatgga gcttgaatcc
     3061 aaggtcatta caaccagtgg gttttgtctg aaaatggtgg caaccgcaac gtagttctgg
     3121 ttgttggttc tggcgatgat gaggctatcc cctgtggtga tgccaaattg tgactttccg
     3181 ctttccactt cagccagtgg attgacttcc ggtccgccgg ggatgagggt tacttcgatg
     3241 ttttcatctt tgaaataacc attttcaatg gcggcgtaaa accctgcgta ttccacactg
     3301 gggaaccagc ttaattgcac ggtcacttta tcgggggccg aaaagttcat gttgccgcac
     3361 ccagacaagg cgagggagag aatcgtaatc caggttgtga agacggctaa tttcgaaaat
     3421 cgatttaatg ttttcatgat cgcgtgctcc tttggtgtta aagtcttgca agatggttat
     3481 gaaaggggta acgacaaggt gaaactggtc cctttatcta cttcgctttc cacgttgacc
     3541 tgtccgtggt gatgctcgac aatggatttg acgatcgcca gaccaagacc gttgccttcc
     3601 acttcgatat tgtgtccgct gcgtacgcgg tagaaacgat caaagataaa tggcaggtct
     3661 gcggcaggga ttccgcagcc tgtatctttc acctcgatca aaagctgcga atcactggcc
     3721 tgggttttga aagtgatttc cccatcggtg ggggtgtatt tgatggcatt tccaagcagg
     3781 ttgcgcagca attgacgcaa ttgcaaggga tcgccactta tggtgacggg ttgctcaaga
     3841 ggggtgtagg aaaaccgctg gtttttgata tttgcctgcg gtatgaactc gttgaccacc
     3901 tcggtcatta ccttgtccat gtccactact tcgtgctttt gggtcgcatc caggtccatt
     3961 tgtgcgagcg acatcatgtt ttgcaccagt tcgctcatgg tgtgggtggc ggtttggatg
     4021 cgggttacaa agtctttttg ctgctgattg agcggccctg catgttcgag cagggtgctg
     4081 aacccggcaa tggtcgtgat cggatttttt aggtcgtgtg aggctgtggc gatgaattcg
     4141 ttcttgacct gttcaaggtc tttgaagcgg gttacatcgt gcagaatggc aacctgcccg
     4201 ccatcttcga tgggggtgat gagggcattg aaggtgcgtt ggtcgggcca ggtaatatcg
     4261 cctgattttg ctccatgcgt cgtgcgcgcg tcatccagca tatggatgaa gacatcgtag
     4321 ccgtggtcat gggggagggg ctggttgagg ttggtgctga aatcggtgaa cagcttttcg
     4381 ccttcagggt tgagcaggga aagcttgccc tgtgcatcga acaccagaat ggcttcggcg
     4441 gcatgcttca gaaccgccga aaggcgattc tgttcctgca tggcaatgga gtacaggcgg
     4501 gcattttcaa tggcgatggc agcctgactg gtaatcgcct gcaaaagcag taaatgatcg
     4561 gtgttgaaat aatttacctg ttcgtgggtg aggacaagca ggccgagcag ttccatacgc
     4621 ccgaacagcg gactgatcac cgcagaatgg atgtttgagt cttggttatc gagctgccag
     4681 agtgaattat tgcgtgcatc ggcaatgatc agaccctgat gcgactcctc gacatgtttg
     4741 atgatgtttt tgggaattgc gatcatgctg tttgcagaat ctggtgaggc ggcaggcacc
     4801 tgataatttt gtgcaggacc gccgttggtg ccgaagatga tcatgttgcc ctggattgcc
     4861 cctaacgttt ccacggtgcg cttgatcagg atgttggcaa ggtctttaat atcggtgcgg
     4921 gcgctcaatt ccttgccgat ctcgggcagc aggttcaatt cgcggttgcg gcggcggatg
     4981 atgtcttccg tctctttcac acgcagtttg gtgcgaatgc gtgccatcag ttcatggcgg
     5041 tcgaaaggct tggtcacata atcgtctgcg ccgaggttca aacccgattg gacatctgaa
     5101 ggatcgaggc gggctgcggt gaggatgatg accgggatat gctgcaccga aggatcactg
     5161 cgaatctctt cgaggacggc aaaaccatcc ttgtgcggca tattcacatc caaaaggacc
     5221 agatcgggca attcggcccg gactttgtcc aaagtatcca gcccatcgct tgcggcaatg
     5281 tgctcgtacc cttcgtattc cagataacgc gtcaacaggg tgacattgtc cggcctgtca
     5341 tctgccacaa ggatcttgaa cttcttccgc agcaccgacg attgtttaat ggggtgcgtg
     5401 gaatgtttca tttcctcgat ggcatttgtg gcgatctggc aaatgcgttc aggacgcacc
     5461 ggcttgacga tcacttcatt gaccttcaag cggtgagcgg tgaccttcag ccctggcacg
     5521 tcatatgctg tgacgagata agtgtatgat ggcctgccag cagggtggtt ttgcattttc
     5581 tcaatgagtt caagcccggt catttcgggc atgatcatat cggtgaacaa aatatccacg
     5641 ccgttggttt ttactttttc cagggcgtcg tgcccgctcg aggctgagat gacactcaca
     5701 cccgtaccaa gctgggaaag cgcgcgagcc agcgtggttg cggtgttcgg gtggtcatca
     5761 acaacgagga tgcggacagg gtttgtactg gtgggggtgg tttccggagg tatcatccat
     5821 ggcattttcg ttcaaaattg cacattcaac gatagtcatc tggatgattt ggttggggaa
     5881 agttggcaac cctgattcgg ggagattcgg tctgggaatc aaaaacccgc ttccggctaa
     5941 gctggaagcg ggtttaaatc caattaacag ggttggcgcc gatattttaa tctgccacca
     6001 gacggtagcc cgtacctcgt atgttgatca ggtaggttgg ggaatggatg tcgggctcaa
     6061 tggcctgacg cagttgatga atgtgccact tgctgagctc ttgcgcttca tggagatcgg
     6121 cctggtaccc ttgggcttcg gtcacaaggg tttgataact gacgacgtca ggggagtgcc
     6181 ttgccaacac cagcaggtat tcaaacgaag tgggcggcag gttgactgcg tgttcgttga
     6241 tgttgaggca gcgtttatgc aggtcgagca ccagtgtgcc gcgtttgatg tagcggttcg
     6301 catctacacg ggtggctggg agcgggccag tatcgccatt attgaggcct tttagttcgg
     6361 cttgcaagtt ttcgatctgt tgcatgatct caagcttgcg tttttctttt tggcggctgg
     6421 cgatgatttt ctgggcgttt tcgatgacga tctgcggttt gagcggtttt agcaggtagt
     6481 ctgtggctcc gctgcgcagg gcttctacgg ctgaatttag atcaggttgg gcggtaaaga
     6541 gtaccaccgg caaggttggg tagttggcat ggatgagttt gagggcatcc agacctgcaa
     6601 tgcccggcat ccgcaagtcc atgaagacga ggtcgaagag ggtggtttcg atgaaggcga
     6661 gtccctggtc agcgttttcg gcggtggtgg cttcgaaacc cgcttgctgc aaaatgcgtg
     6721 ccagactttg gcgcagggtg gcttcgtcgt caatgatgag gatgtgcccg ttgctcatag
     6781 aatttctcca ctggtgacgg gcagccagac acggaaggtt gcgccctgat cgtggttgtt
     6841 ttcggcggtg atgcgcccac ggtgtttcat gatgatgtca taggtgatgg tgaggccgag
     6901 acctgtgccg ctctgtttat cggtgacaaa ggcttcgaag atggtgggca atatcttggg
     6961 tggaatgccc ggaccattat ccgacatgat catgacaact tccccggtgt ctttttggta
     7021 ttcggtgcag actttgatcc tgccgccatt ttgcatggct tcactggcat tcatcaatag
     7081 attaagcacg acctgacgta tctggtctgc aagcgctggt atgaacggga ttgcagggtc
     7141 gggggtgaat tcgaagctga tctggttgtg gcgaagatgc gtggagacga gggcgtacac
     7201 gtcgttcaca atgtcgttga gttgggtggg ctgaaaatct tccgcgtggg tgggacggta
     7261 ggtgtcacgc aggcgtgcga tcatgttggc catgcgttcc gattctgcaa ggacgatgtc
     7321 gaggtcttgt ttgccttggg gcgagacgtt ggtctcctct ttgaggagga acagggcgtt
     7381 ttgaatggcc tgtaatgggt tgttcaattc gtgtgagacg gtagccagca ggcgtcccat
     7441 cactgtgaga cgttcgtttt gcattaattg gttgcggatg gttttttcct gttgcaggga
     7501 tatttgcagg gtttcgtaca agtccgcttt ttgcagtgcc acggccagtt ggtcggctac
     7561 ggcgctgacc agttgcaggt ctctttgtga gaggtatttg ggcggtactt gatgaatatc
     7621 gagcaggcca aggatctgtt tgccgacctt taccggcact gcaagctcag attttgtatc
     7681 tggcaggagc gggtggcgga caaaagacac cacatcatct acattattgg tgaaaaaggc
     7741 tgttcccgtt tctgcggtgt agccgacaat accctcgccg ggcgcgagtt gatgttcgcg
     7801 tgctttgagt aaggcgccaa ccttgccgct gcctgctcgc atgatgaaat tgcccgtatc
     7861 cgggttggca acataaattt gaacgtagtg atagccgaag tttttttgca ggagttcaac
     7921 tgtatcttgc agcaggtcgt gggggtcaag tgaggctacc aatcccttgt tgacacggta
     7981 cagggcgttg gcttccttaa gggcctgttt tgtattttcc aacaggtttg cgttttcaat
     8041 tgcgatggcg gcttgtgtgc caagctggct gagcaatttc agatcgctgg gagtaaaggc
     8101 gtaggggcgg ctgctttgaa tgctgatggt gccaagcttt tgttcgccgc tgcgcaccgg
     8161 tgtgaccaac agggaacggt aggcgggttc cactccaagc cttagaaagc gcgggtctgt
     8221 gcggacatcg gcgatattga tcgtctcacc actgagaatg gcctgcccag ccaccccttc
     8281 tcccagtcgt atgtttctct ttccaccttc aagctctgtg tacccaacca cggcctctga
     8341 aacaaggatt tggtcctttt cgttcaatag gtggatgaca gcctgttcgg agtttgcgat
     8401 caagtccttg gcagatttta cgatcagatg caatacattg ctcaagccaa tgcgttcagt
     8461 ttcgctcaaa gccagagaaa tatcagccag tgattgcatt tcccgtaacc cgcgttgaag
     8521 ttcgttatcc tgttgtaaat tccaaatggc cagggcgatc tggttgccgg cttgttcggc
     8581 gcggttcaat tcctcttcgg tgaattgatg gggctccgca aaccccacaa tgactgcccc
     8641 aagtttgtga tcaccgtaat tcaaagggat gctgataaat gatttttcag gaaagaggtg
     8701 aataagggtt gcacttacat aggtggaatg atgcgggtct tccacaacga tgatgcggtt
     8761 ttcctgtaag gcggtaatgg tgaggttggc gtcccctttt ggatactcca tggtgaggaa
     8821 ggccgtgttt ttcaggttgt tggttgctac aggaaatacc tgctccttga ccgggtccca
     8881 acgggtgata tagcaactat cagcatccag cagggtggtg atgtcattcg tcagcgtgtt
     8941 tgccatgctt tcgaagttct gtgctgtggt gatggcatgg gccatgtcgt tcagtagttc
     9001 gagaaaacgc tcacgggagc gtaattgact taagatgctt cggtattctt cgtatttgta
     9061 tttgatccaa ccgacgctga tgcccagtag tgtgataaga atcgcaccaa gcccatagtc
     9121 atatccattg atctctgccc acggaatctt ctgaacgtta accaacaata ctgtgttgac
     9181 aagaatcgtc aacaagcttg caataattcc accgggaatc ccaaaaaaat aggcgctgac
     9241 caccaccggt aagccgctca acgtagtgat ggcaacgcca aagtagttaa acaacaaaat
     9301 ggaaccgatc aggtaaatta ctgctgaaag gccggtaaac agccattttg cctgagatga
     9361 ttccaagtat cgattcgttg ttggttttgc gtttgggagg gccataaact tggtttatta
     9421 taatgcgcct gtttggggcg gcacattgtg aaatccttgt aaatgaagcc tccacctttt
     9481 aggtgggagg cttgaaactt cccaatcaat caaaaagccc aaagaaatat cctttgggct
     9541 ttccattttt gttttgttta ttttgcgtat tcggttgccc gtgtttcgcg tatgaccgtg
     9601 accctgatct gaccgggata ttgcatggtc tcttcgatct tcttggcgat atcgcgtgcc
     9661 aatctggcgg atgagaggtc atcaatggta tctggtttga cgaggatgcg aacttcgcgc
     9721 cctgcctgaa tggcgaaggc ttgttgtaca ccctcaaaag acatggacat ttcttcaagg
     9781 gtgcggatgc gtttgatgta ggcttccaag tcttcacggc gggcaccggg acgggcgcca
     9841 gaaatggcat cggctgcttc ggcgatcaca gcttcgacag tttcctgatc cacctcatgg
     9901 tggtgggcgg cgatggcatt gaccacggtg gggtggacgc catagcgttt gcaatattct
     9961 gcgccgaggc ctgcatgggt accatcctgg ttatgatcca tggctttgcc aatatcatgc
    10021 aggaagccgc cctgtttggc aagctcgata ttcgcaccaa gctcggccgc gaggatgcct
    10081 gcaagtttgg aaacctccac ggcatgggct aactggttct gcccgtagga cgtgcggaat
    10141 ttcaggcggc ccatcatgcg gagaacttcg gtatgtaagc cggtgacatt ggcttcatag
    10201 gcggcttgct cgcctgcttc gttgatgatc ttttcaacgg ctttggtttc atcttccacg
    10261 atcttttcga tgtgggcggg atgaatacgg ccgtccaaaa taagcttgga aagggcgcgg
    10321 cggccaattt cacggcgaac agagtcgaag caggagatgg tgaccgaatc aggtgtgtca
    10381 tccacgatca catctacacc ggcggcctgt tcgaaggcct tgatgttgcg tccattacgg
    10441 cccacgatgc ggcctttcat ttcctcgctg gggagggtga ccactgcacg cgttacttcg
    10501 gcgacatgtt cggatgccac acgttgaatt gcatcggcaa tgagtttgcg ggcgcgtttt
    10561 tcgccttctt cgcgtgcttc ggcttcgatc tggcggatga tgcgggccat atcaccgcgc
    10621 gcttcctttt caaccagggc aaacaattcc ttgcgggctt cgtcttgagt catctgtgcg
    10681 atgaactcaa gttttttcat ttggtcatcg taaagcttgt caatctcgtt tgcgcgtttg
    10741 tcaacctgtg actggcgctt gttcagcgtt tgttcgcgct gttccatttt gtcgaagcgg
    10801 ctatccagtt cgctgcgacg cttgtcaagg cgttcggttt ccttgtttag ctcaatgcgg
    10861 cgttccttga cctcagcttc ggcggctttg gtgatctttg cggcgttttc gcgtgcgccg
    10921 ctttcgatca ggcgggcctg tgagttggcc gcctttaaaa tgtcatctgc tttttcctgc
    10981 ctggctttcg cagcttgttc ggcttgatat cggtgaaaga aatatcctgc agaaacaccc
    11041 acgatgagca caattaggtc aataattagg acgagcgggc tcatgttatt tcctcatttt
    11101 catcgtcaaa aattttttgt tccccagttg gatgagtttc actccatgcc ctggtgacga
    11161 cgggggcgat gatggagtag gggaatcctc gtcttgcgag gaactcggat aatttttttc
    11221 taaagtcgtt ccattccaga cctttgagcc tgcccagcct tttttgggca gcttcgtaag
    11281 ccagggtttc ttcgtccact gctgatactg cggtttggac ggtttcgtca ttgaggcctt
    11341 tttggcgtag ttccattgcc agagcccgcc gacttcgtgg gcggaaggtg ctgcggtttt
    11401 ccacccacgc ctgtgcaaat tcatcgtcat tgaccagacc tgcatcacgg agacgttcaa
    11461 tggttgtttc aacaacgtct tcggaatatt cgtgcttgtg caggttctgt cgaatttcct
    11521 tctcagaacg ggcgcggtag ctcaaaaaga gcatggcttg ttgatatgcc cgttcacgtg
    11581 agtcttctga aagcaggctg gcgatctttt catcgctgag ttcgtcgcca actttcaacc
    11641 atgcggccgt gatacgcgcc actccaaagg cgaactctcc atcaagatgg atgtttaccc
    11701 ggttgggggt cttcttttga accgaaatgg cggttatctt tttcatttgt taaaaatcta
    11761 cagccaaggt ctccgaccag tgggaggtcg gccttggctg tggaaaatgt ctgggttgac
    11821 tgagcaggcc caagtccgtt tgaggcggat gaattggggt aggtcagagt acgtgagcaa
    11881 tcgtcacgtt tggcatgggt tgtggggggt gaagccagtc tagggtgttg gtaatattca
    11941 tttagaaaga cttcacaaaa taactcccaa acgcttgggg cgggggaggg atcgagcgcc
    12001 aaatttccag tcagatttca agcaaggccg cgaacggcgg agttttcaat gtcaaaccct
    12061 tacggatttc cttaattata catgaatttg aagatttgtt aacatttaga atccatatta
    12121 aatcactcgc tggccttgaa atgatgcctg ttttattgag tataataaaa tttcttccca
    12181 atctggttta tgaccatgtt ttgacctttt taactgaaag cagtttctca aatactggag
    12241 gaatcgatga ctgaacaaca acaagaagcg actgggggag atattgggag gatccgacgg
    12301 ttcattgctt ttctgggcat cctttttatt ttggtgagcc agtttttgat attctcccag
    12361 cctgttgatc atacgttggt gtttccgcct tatacgtggc tggcgattgt aggggtgctt
    12421 gttttggctt tgggtctttt tatccgccca acggcgttct ttcaacggat ttctgactgg
    12481 ttcgtttttc aagagcggac cttttgggtg ctggctgctt ttttaatgtc ggctttggtg
    12541 accatggcaa cggccaatct gatgttgttt gcccgtatta attacattcc cgtcctcacc
    12601 atttggttat taagcgcctg ggcttatatt tatgcctttt cgaatttttc cattagtgga
    12661 tctgcgatct ttgattggtt caaaaagaac cgcaatgaac ttttggctgt tttggctgta
    12721 accctgttgg ctgcagggct tcggttttac aggctgggtg gtattccgcg tattttggat
    12781 ggtgatgaag gtctgatcgg tcttgcggct caaaccacaa ccagcgggtt gctggcgaat
    12841 cccttttcgc tgtgggaaaa cttcggggcc atgtatctgc atttgatcaa taccgccatt
    12901 ggtttttttg gcgtgaatgc ctttgctttg cggttatggc ctgccattgg tggaacgctt
    12961 gccatcccgt ctttgtatct tttcgttcgt tggatcgggg ggcgaaggat tgctttgatc
    13021 accgccttga tcattgcttt ttcacattcc catattcatt tcagtcgaat tgtgtctgtt
    13081 gcgtatatcc acgggacgtg gcttgctccg ctggaactgt atctcatgct tagcgggttg
    13141 gaaaaacgcc aatcatggcg ggctgcgctg ggtggggtgt taattgccat tcatttttcc
    13201 atttatttga ccgcacaggt gatcattgct ctggcttttg tgtttatggt gttggcattt
    13261 ttgttttatc gaccctggtt caaggagcgt tttgctcagg caatggcttt ttggggtggt
    13321 tttttaatta tcatcctgcc ttctgctttt tatatttatc gaaatcccaa cgagtttttt
    13381 aaccgtttgg ctcagaacgg cacatttcaa tcgggctggc ttgagttgac catgcaatct
    13441 acggggcaaa gcgctgccga aattttattc ggccgctttg tacatgcctt cctttcacta
    13501 acttattacc ctgccattga tttctatggt tcatcctcgc cgatgatgag catgatttca
    13561 agcaccatgt tcctggtggg gttgctggtt gccttatggc gaattcgaaa tccagcctat
    13621 ttgcttttga acggttattt ttggggggct actttctcgg ttggtttgtt cgccattcca
    13681 cccaattcag attcctatcg tatgttgatg gcgcttccct cagcgttgac tatggctgcc
    13741 atggggcttg accagatcct tgaattgctg ggcctggggt ggaataaagc ccgcacctct
    13801 tacacacttt ccgcatcggc gattcttgtg agcctgctta ttttcaacct ctggacgtat
    13861 tatggcgact ttgcaggtaa atgccgcttt ggtggtgatc tggtggggcg atttgcttcc
    13921 taccttggca gttacgcaca cagcgtggat aacgaacttc ccatctattt gctgagtaat
    13981 gggatttatt tctatggcag tcacgcctca actgattttt tgagcgagag cagggcgatc
    14041 acaaactttc ctgacccgat tgatactttg cagcccgtct ctggagaaac aattatcgcc
    14101 aaccccgaac gcatccccga gttggaagca tgggcacgtc tgaaccctgg cggcgaattg
    14161 cattatcgct acgattgctc aaataccatc ctgttggctt atcgggttcc ctagaggaga
    14221 cttggatccg cgcgaatacg aacttatgta tcaagtcgaa gaccgtcact ggtggtatcg
    14281 tggaatggaa accatcacac gggctctgct tgaacgctgg gctccagcac gccctcaaag
    14341 aattttggat gcaggctgcg gtaccggcgc cgccatgaca acctacctca cagattatgg
    14401 aaaagtgacc ggtattgact tgtatgccga agccctgaaa ttttgccgcg aacgaaatgc
    14461 tccacgcctc gcacgcgcct ctgtgctgaa cctgccattt gctcctgctt cctttgacat
    14521 ggtcaccagt ttcgatgttc tttacgagcg ggccgtctcc gatgaattga ccgccttgaa
    14581 ggaatttttc cgtatcctga ccccgaacgg gtgtgttctg cttcgcctgc ctgcctatga
    14641 ttggctgcgt ggacaacacg acagaaatgt gcataccaat cgccgctaca caaagaaatt
    14701 ggtcaggcat ctgcttgaac aaagcggatt tacggttgaa catctttccc atgccaatac
    14761 atttcttttt ccccttgccg tgatgaaaag aatgggggag agggttttcc ctcccaaaga
    14821 cgatcaatct gaccttgcca tgaatgcagg tgtattcaac gatgctttga agatcatcct
    14881 ttctgctgaa gcccctcttg tctcaagcat tggcctgcct tatgggttga gcgtgatcgc
    14941 aatcggtaga aaatgacaaa cctgatgaag catgaaagag aaagccagca acttcccagt
    15001 atcagtgcgg ttttcccagc atacaatgac ggcgggacaa ttgccagcat ggttgctgct
    15061 gcctggatcg ccttgaatca ggtcacttct gattacgaga tcatcgtcgt aaatgatggc
    15121 agttccgact atacagccac catgttggat gagatatcgg ttcgatatcc tgagctgcga
    15181 gtgattacac atccgtataa tcaaggctat ggcgcggcat tgcggactgg gttttccgcc
    15241 gcatccaaag attgggtttt ttacaccgat ggcgattcgc aatacaaccc gcttgaactt
    15301 gtgaatttgg ttggcgcttt gtacgaaggc gtggatgtgg tcaatgggta caagctgacc
    15361 cgtaacgact catggatgcg gatcgtgatc gggcgggcct atcattacct cgtcaaattt
    15421 cttttcggca ttcatattcg cgatgtggat tgcgattttc gattgattcc ccgccgcatt
    15481 ctcgaagaga ttgaattgaa aagtgtcagc ggcgcgatct gcctcgaact ggttaaaaaa
    15541 attgaggatg cggggtatgt ctttgcggaa gttccagtca accattattc acgcaaatac
    15601 ggagtctcgc aattttttgt cccctggcgc attgcccgta ccctgtatca actggcggat
    15661 ttatattgga aactggtgat tcgaaaagag catttaatta aacgatgacc attatggata
    15721 ttgctgctgc gtattttcgc caaaaagtat tgatcacggg gggagcaggt tttattggaa
    15781 gtaacctggc gcataaactg gcaaccctcg gtgcagatgt caccattgtg gatagccttg
    15841 accctgattc tggcgccaat ctctttaact tgaacggcat tgaaaagaca gtccgcttga
    15901 ttcaggcgga tatcaatgac gagaggcaga tggctcaagc agttagagga cagaaattca
    15961 tgttcaacct tgcggggcag accagccacc tgggaagcat gcaggaccca ttcaaggacc
    16021 tggacgtcaa ctccgtgggg cagttgcggc ttttggaagt ttgcaggcag aataaccctg
    16081 aaatcagtat tgtgtttgcg gggactcgtc aggtgtatgg gcgcccaaaa tacctgcctg
    16141 tggatgaaaa ccatttgttg acccctcttg ataacaatgg cgtcagcaaa cgggcgggag
    16201 agatgtatca cattgtctat aaccgtgtgc atggaatggc aacctgtgtt ttgaggatga
    16261 ccaatgtgta tggcccacgc atgcgcatca aagatgatct cctgaccttt attggctggt
    16321 ggtttcacca acttctgatg ggagaaaata ttcagatatt cggcgatggc aggcaggtca
    16381 gggatatcaa ttatgtggat gatgtgattg atgccatgtt gttgtgcgcg gcgcatcctt
    16441 ctgcaaacgg caaaatatac aatctgggcg gggcaccgat caatcttttg gatctggctc
    16501 gtttgatgat tcaagtaaat ggcaaaggtg gatacacact tgtgcctttt cctgagaatc
    16561 gcaaacgcat tgatattggc gattattacg gcgattacag cctcattcaa aaagatgtcg
    16621 gctggcggcc gcaggtggaa cttgaaacgg gtatttcaaa cctgctggcc ttttaccgca
    16681 tgtataaaga acattatttg tgagtgaaat ggaagagatt cccgtcattg acctgaaaaa
    16741 acaatatcag tccattcgcc ccgaattgga tgctgttttt gcccgtgtga tggaaaaagg
    16801 cacttttatt ttgggttcgg aggtttctgc ttttgaaagg gaattcgccg aatattgcgg
    16861 cgcggctcat gcaattgggg ttgcctctgg tacagatgcc ctgcaattgg ctttgatggc
    16921 ttgtgagatc ggcgaaaatg atgaggtggc tgtcccggct cataccgctg tggcaactgt
    16981 cagcgcggtg gaagcgggcg gagcccatcc gcttttggtg gatattgatt ccacaaatta
    17041 cacgcttgac ccaaatctgc tccatcgaat tttgatgccg cacactcgtg cggtcatccc
    17101 tgtgcatttg tatggctgcc cggtggatat ggagcctgtg ctccaatttg cgcgcgagaa
    17161 tcatttgttt gtgatagagg attgctcgca ggcccatggc gcttcgtacc gagggcgcaa
    17221 ggttgggacg tggggtgata tcgccgcttt tagtttttac ccgactaaaa acctcggcgc
    17281 gtttggtgat gggggaatgg tggtgacgaa taacgatgcg ttggcagaga aggtgcgttt
    17341 gctgcgccag tatggctggg gtgaacatta catcagttcg attcggggaa tcaacagccg
    17401 ccttgatgaa ttgcaggcag ggattttgcg cgtcaaactg cgtcatttgg atgggtggaa
    17461 tgcacgccgc aggcagcttg ctgagttgta tcaccatcta ctttcaggga cagatctggt
    17521 tttgcctgcg caaaatgcgg gccatgttta tcatcaattt gtgattcgcc atgcacaacg
    17581 ggattcgttg cgggaatatt tgaaggaaaa tggtatttcc acactgatac attatcccgt
    17641 gcctgtccat tttcaacctg cttatgctga tctgggatat atagcgggga gtctaccgtt
    17701 cacggaacgg gcctcccgtg aagttttgtc tctgcctctt tacccggaat tgaccgaaga
    17761 aaaggtggtg aggatttgtc agaccatatt ggattttttc acgcactcaa tgtgacattt
    17821 ttcatgcctg catgacagaa cttttggctc gattggggta agtatagata agctttgttt
    17881 gtggctctcc aaagttgtga caaaaggaac tattctcttt aggcaagtta tgcgataatt
    17941 tacattaact gtcgtatttg aaactcttcg actttcaaaa tttcactgga ggcactatgg
    18001 caaaaatcac gcgcgaagat gcacttgaat atcaccgttt aaagggcaag cccggcaaag
    18061 tggcgatcgt tccaaccaag ccgatggata cacagcgaga cctgagtttg gcatattctc
    18121 ccggcgttgc cgagccagtt ttagagattg aaaagaatcc cgacgacgct tatgagtaca
    18181 cttccaaggg taatttggtg gctgtgatct ccaacggaac agccattctc ggcctgggtg
    18241 accgcggcgc attggctggc aaacctgtaa tggaaggcaa gggcgtctta tttaagaaat
    18301 ttgcggatat tgacgttttc gacatcgaat tgaattcaca cgacccggat gagatcatca
    18361 aagtagtagc ggcgatctca ccgacctttg gcgggatcaa tctcgaggat attaaagccc
    18421 ccgaatgttt ttacatcgaa gaagaactga agaagatgct gaacatcccc gtcttccacg
    18481 atgaccagca cggcacggcg atcatttcct ccgctggttt ggccaatgcg ttggaaattg
    18541 tcggcaagcg gcatgacgag atccgactcg tcatctccgg ggcgggtgct tcggcgatct
    18601 cctgtgccga attggcgatc agctggggcg tgaagcgtga aaatatcatg ctttgcgaca
    18661 gcaaaggtgt ggtgtacaaa ggccgcaaag agggtatgaa taaatacaag gagcgtttcc
    18721 tggtggaaac cgatgcccgc acactgactg acgccctgcg tggggcagat gtattctacg
    18781 gcttgtctgt agcgaatgtg atgacgcccg aaatggtcaa gttaatggca aaggatccga
    18841 tcatctttgc catggcgaac cctgacccgg agatcaagcc tgagctggcg agagaagcgc
    18901 gcaaagacgt catcatcgca actggtcgtt cagattatgc caatcaggtc aacaacgtgc
    18961 ttggcttccc cttcatcttc cgcggcgcgt tggatgtacg tgccaaacaa attaatgaag
    19021 aaatgaagtt tgcggcttcc aaggctttgg ctgcccttgc taaagaggat gtgcctgatt
    19081 cagtcatccg tgcgtatggc ggcgagagca ttaaatttgg gcgcgagtac attatcccca
    19141 agccgttgga tccgcgtgtt ttgttgtggg aagcgcctgc cgttgctgaa atggcaatga
    19201 agaccggcat ggcccgcaag accattgaca ttgaagaata caaggaacag cttgccttcc
    19261 gtcagggcaa gggtgaacgg atccgctact tcttccaaaa caaggcccgc acctcgggcg
    19321 gcaagaagcg tgtggccttt gctgaaggtg aagagcagaa ggtcattcgt gcggcctatc
    19381 agatcaagga agaagggatc gccactcctg tcttgatcgg gaacgcgggt gtcattgatg
    19441 aacaactcaa ggtccttggc ctcgactata agcccgtgat cgtggatccc aacaaattcg
    19501 aaaaattgga agcctacgcc aagtcctatt acgaaatgcg ccagcgcaag ggtgtgatgt
    19561 tgagcgatgc acataacaaa gtgcgcgatc ccaatattct tggtccactg atggtgaaaa
    19621 tgggcgatgc agatgctttt gtctcaggtt tgacctttga ttatcctgaa gtgatccgtc
    19681 ctgcattgca gattcaccat actgctcccg gcgcaacacg tgcggcgggc gtgtatatca
    19741 tgatcgtgga tgataaggtc ttcctgttta cagatgccac cgtcaatatt gacccaaccg
    19801 ctgaggatct tgcagagatc gcctctcttg ctgctgattt tgcagtgaaa cttgagatcg
    19861 atccgcatgt tgccttcctc tccttctcga acttcggctc caccccacat ccgctttcag
    19921 acaaggtccg caaagctgtg gaattgacca agaaacgccg ccccgatctg cgtgtggatg
    19981 gtgaaatgca ggccgatacc gcggttgtgc ctgagatcag cgaagaacgt tacccattca
    20041 gcgctgtgaa ggatgcaaat gtgctggtct tcccctcgct cgaatcggcc aatattgcct
    20101 acaaactgct ttcacgcctt ggtaatgcca aagccattgg cccgatcctg ttgggcatgg
    20161 gcgcacccat ccacgtattg caaactggcg atgatgtgaa tgccattgtc caaatggctg
    20221 cggttgccgt catggatgtg atgggacgtg aggcgaatgg ttaaagaaag tggttttacc
    20281 tggccatatt gaaaagtgaa agttgactac aaaaacactc tcaggatatt ttttcctgag
    20341 agtgtttttg taggcgattg ggaatagatg gaacattatc tgtttcttga attaagatca
    20401 aaagcctcca gcattttttc aacttctccc attgtgtaaa caaaatgagt gacagttctt
    20461 cgaaaaaaag ctttttctac aatgtcaaac gcgacctcaa gcatataatt tttgctttga
    20521 ttgacaacaa aacaggcacg taaattagct ggcggcagcg gaagtttccc ccacaaatct
    20581 ttaacagcgc caatggtgga ttgttcagga ggaaatttag actggtcatg aatgatgatc
    20641 gccttatggt caattgattt gaaataatgc tctgcctcct ttagcgcatt tactacgtca
    20701 ctccactgcc agggctttaa atactttacg cttaacactg tctttgttcg tgggtcttgc
    20761 cactcaatta cgaaaatatc attagagttc attgcaattt ttcctgtagt ctacctggtt
    20821 caggtaaaaa atctgtgttt ttctgtgtct caaaaaaaca aactgacggg tgactggagt
    20881 tttttgccac cccaattaaa tgtgccagac actttggtca tctaatattt tttcaatgag
    20941 ttttggaaaa gcgtacctgt ccaatggttt tgaaatggct ccatcaaaac cgctttcccg
    21001 taatttttcc acttcctcgt tcattacact tgcagtcaat gccactactt tacagtaacg
    21061 caatcgatta tcagatctga tcattttgag aagttcgaag ccatcataag gctgtatgtg
    21121 gatatcgagc aaaaagatat caggaatact gccaatctcc ctgaccttct ctagaaaatc
    21181 acttgtgttt tcaatgacga tcaagttgtc aacgatgcca agtacatggt tcataatagc
    21241 tttcataaca acgatgcttg gggcgtcgtc ttcaacatat acaatgatgg gtccgttttt
    21301 catgattacc tttgcgataa tattgggtat gtggggattt ggggataagg gagggagaaa
    21361 tagaatacag aaccttcatt aggctggctt tcaacccata gcttacctcc atgcgcttca
    21421 accaggtttt tcgaaattgg aagcccaagt cctgttcctt caactctctt acgaccatct
    21481 tctgtttggg tgaagggttc gaagatgatc gtttgtgcgg taatgggaat gccgggcccc
    21541 gtgtctgcta cggcaaaaag cagatagtcg gctctttttt tgagaataag agtgatgctc
    21601 cctttatctg taaatttagc cgcatttgac agtagattaa gcaacacttg acgaatgcga
    21661 cggcggtctg cttcgatgag tggaatatcg ctgtcaatat cctgaatgac ttcaacagac
    21721 tctttgatta atggggtgga cattttgagg gcagtatcga tctcttcgta aatgttgacg
    21781 tcttgttcaa ggttgagcgt gagttgtcct gcttgaatct tgctgatatc cagaacgtca
    21841 ttgatcaagt gcagaagatg gtgggagctt cgcagggatt gattgagaaa agcctgttgt
    21901 tcagtgttga ttgggccaac catttcagac gcaaccaggt cgatcaggtt gatgatggcg
    21961 ttcatggggg tgcgcaattc atgactcatg ctggcaagga attggctttt catgatatcg
    22021 gtccgtttga gttcttccgt gactttttcc tgatttcgat aaagttgggc attacgtacg
    22081 gcaattgcga tctgttcggc aagtgtggag aatatccgaa tatcattatc cttgaagcgg
    22141 ccgatggttt cagcttgtaa atccagtacg ccaacaagat catcaccaac aatcattgga
    22201 aatgcagcat gtgattttat tgacggaagg cgtaatgtgg gcacaaagga ggattgatct
    22261 atgttgttta aaacctctgt tttgcgagtg cgtgccacct ctgcggtaag gcttggccga
    22321 gtattgatgt gaaatgactt tggttccgtt ttcactccat tttcttttac atcgtcagct
    22381 tcgagctgca gttcgtccgt ttccggctgg tataagaata tcgaagtgga atacagatcg
    22441 aaaccctccc gtgtctgctt gaccagattt ggcagtagtt tctccagttt caattcctgt
    22501 gtgatctggc gcgacacatc agttgccatc tgcagatcac gagtacgttc ggcgacatgc
    22561 tgttcgagag attcgattaa atcgctcaac tgagcgctca tggagttgaa ggtttgtgag
    22621 agcgtggaaa attctttcaa gccgtggata tcgagatttt tttcgaaatc gccctgcccc
    22681 agacgttcgg ccgccagggt gagttgattt aatgggcgta aggtttggcg ggccagaatg
    22741 ataaatacac caatgaccga cagaagggat agactgcctg ctgtcagttg aattagcaat
    22801 gtggttctgt atgttgagtc gagttcttga ttcgccaata taaggccatt tctgctgacc
    22861 tctccaatgg ttttcgtgat caaatttatg tcaatggtga tatctctgaa aatggtagtg
    22921 ttcttaataa tatcctggtc tagggcaacg atctttgtga atgtatcgta gtgttcgagt
    22981 aggtttgtgg aaagttgttc ggcgtttagc gtctccaaac cgccttcttc agtggcttgg
    23041 gttaaatcca ccagttcaat ggtgttgata cgtacataat ctatatattc ctgcctgtaa
    23101 gtgctgaagt atccctgctc gttcttttgg atctgaatca atgtgtcgaa aaagaattga
    23161 ttgtcgaggc cgcgtgtctc aaattgcatt tcctcgattc cactggcaag ggatttttcc
    23221 agcccattgg ccccaatgcg tgtctgaatt tttcccaagg ttgcattgaa tgagttttca
    23281 taatcaccca atagagcaga taattggaca gtggaattgg cgatttccct gaagttttta
    23341 tttttagaat taattgaaag ggtttgcaat tcattgaggt gggtgcgggc tgcatcaaga
    23401 tggaactgaa tttgctcgcc atattctttg cgggcattat caaagccaag aagccgccag
    23461 tttgcaagaa aagcagattc gtcctgacgg gccagcaaaa attccgtctg gatttccagg
    23521 ctgagttccc tgatttgact gctttcctgc gaagtggctt taagtgcaga ttgaaaactg
    23581 ttgaaattta tcaggctaat gcccatacca agcaggttga atcccaatat tgccataaag
    23641 cccagaagca ataactcacg aactgataac ttatttaatt tcatagacct ggcctttgcc
    23701 aaaatttaaa aggcaacatg gattatttaa gcgcatcatg agcggcaagg atcatttcaa
    23761 atgctgcatc gtagatattg ttggcttcct gcccaacgtc tacaacatag aacaaacgat
    23821 aacggatgtt ggtgggtgga taaatgatgg ggtttgtcag gtcgctttgt tcaataaatg
    23881 gcaatgcagc ctggttgggg gatgcgtatc gaatatagtt tgaaagttga gctccgatat
    23941 gtgggtccat catatagttg atcagctttt cggtttgttc tttgtgcgga gaagcaacag
    24001 agatgcacat attatccgtc cataagacag ccccttcttt ggggataaca tacttgatgt
    24061 cggggttttc tgctgcaatt tgcataacgt ctccgctgta ttcaaccgcg acatctacaa
    24121 tgccattgac aagcaaatcc tgcccgtcat caccacgcag tgtatagaat tgatcaatat
    24181 gttcgatcaa ataatcgcgg gcctgatata tctcactttc attggtggtg ttcgggctaa
    24241 agcctaaata taaaagcacc cctcccacag ctgtgcggtt gctttccaca aatgaaacgc
    24301 ggcctttaaa ttgaggatca aataaatcgc cccagctgtc gaggtctctc cctgtcgctt
    24361 ttcgattata ggcaattgcc gtagttcccc attgatatgg aacacaatag cggttgtcag
    24421 ggtcatattg tgggcttacg aaaagaggat caaggttctt catattcggt atgttggctt
    24481 tgtttaaagg agcaagcagg ccatcccatt gtaagataga gactgcataa tctgaatcaa
    24541 taaacaagtc aaatgcttcg gggttttctt taagcctttt tcttaaatcg cggcttgagg
    24601 tatatgttcc ataattgacc ttgatgttgt attccttttc gaagttggaa agaatattgg
    24661 ggtccatata attgtcccaa ttgtagaagt ttatttcttc cggggaaact tctgcttcag
    24721 gcgtttgttg ttgtgaccag ccttgacatg cagaaagtag tgccatcaag gtgagtatgc
    24781 taagacagag aactggttta agaatctttg tgctcatatt tggcttttac tctcttccat
    24841 aagatagggc aatgtgaaat aaaaagcggt cccttcacca agttgacttt ccacccacag
    24901 cctgcctcca tgtgcttcga caagcttttt tgaaataggc agtcccaaac ctgtaccttc
    24961 aactctctta cggccatctt cagtctggac gaagggttca aaaatgatcg tctgtgctgt
    25021 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngatttcttc atagatgtta
    25141 acatcctgct caatgtagag ggtcagttgt ccagcctgaa ttttgctgat gtctaggacg
    25201 tcgttgatga gatgcagaag atgacgagaa ctttgcatgg attgattgag gaatgtcttt
    25261 tgttcctcat tgatggcacc tatcatttca gaagcaacca tgtcaacaag gttaataatt
    25321 gcgttcatgg gagtgcgtag ctcatgactc atgctggcca ggaactggct tttcatcagg
    25381 tcagtacgtt tgagttcttc agcgactttt tcctgatttc tataaagttg ggcgttgcga
    25441 atggcaattg caatttgttc tgccaatgtg gagaaaatct gaatatcgct ttcggcaaag
    25501 cgatttggat attctgattg aaggtccaat actcctacaa gttcctctcc aaccaccatg
    25561 gggaagacag cttctgctct ggtgtttggc aggagtgggt tgaaaaaatg cgaatccgat
    25621 ctggatgtgt cgtttacgat ttcagctttt tgttctcgtg cagatttgga aaccaggctt
    25681 ggttgtgcag agatatgaaa ggcttttgcc tgacctttca tatgttcccc ggatgcacca
    25741 gaacccgcct caaacaataa ttcctgggtt tggggattga aaagaaacag agaaacagca
    25801 tacagattaa aggctaccct tgttttttcc acaagattag ggagcaggtt ttctagattt
    25861 aattctcggg tgatatcccg cgaaacttga gaagctacgt tcaaatctcg cgtacgagaa
    25921 gcgactatgc tttcaagtcc attgagagtg ttatagagtt gttcaaccat ttgattgaat
    25981 gcaatcccca gttgaccaac ctcatcgttc atttcaattt ttgtacgcct ggaaaaatcc
    26041 ccctgcttga tcatttctgc gttttcagtt aactgtatca gaggggtcgt aagatatctt
    26101 aacacccaaa cagttgtcag ccagatcacc ccttctatgg tcaggagaat catgtgtaat
    26161 tcttctggaa tattggcaat aaacgcgaaa gcagtaaaac caattaaagt aagttggatc
    26221 tggactacca gaattttccc tgcaagttgg atttgtttga aatttagaaa gaagggtatt
    26281 gaataggcta tatctgatgc aataaccgta ccgccaatga tccagaaacc ccattttgga
    26341 atggctaccg aaaaatccaa aaaaatggcg gtaaaacaca gtgaaagata gattcccaga
    26401 tgaataagga tattcctgaa agggagtctt tctggataca aagtaataaa taaaataatg
    26461 tgataaattg cagctgttaa catgagcagc ggcatttcgg agagaaaaaa gcaccctaga
    26521 ccaggggcaa accagtagaa tgtggttata acccgaagtg cgatgtagtg gttgttgttt
    26581 gcagcttgtc tgtatgaaag ccagcctcca accccataaa ccatccctgc cgtgaaaaag
    26641 caaagtagtt gccacgaggg tgattgacta taaatataaa taggtaaaat ggttacaaaa
    26701 ggtgtaaaac ccaccattag taatgaaacc cattttagat actgataccc aattgaattg
    26761 gcaggtaatg taggtaaaga agaagcattc atacttgaat tttgctttta gtgaaagttg
    26821 tagaaggcaa ttattgactg ctgatccatt tcttttggcc tgcgatgata tctgcaatat
    26881 gttgtgcaaa cccatgaacg cggatgggct tgctgatgaa tcccgaaaat cccatggctt
    26941 ttgccttcgg aatctctgtt tcgggatcca acgaagtgat ggctataaca ggaatatgtg
    27001 ctgttttggg gttttgctta atttctttga aagtatcgta tccattgaca cctcttttta
    27061 acatgatatc caataagatg atatcaattg gaagatgctg ctcaatatgc agagatattc
    27121 caatgctgtt gtagttatag aagactgatg ccccggaaac cgacaaaggc ctggaaatga
    27181 cactcaggtt taatacatcg tcttcaacta caaatattct tgtgttgagt agtggcaggt
    27241 tggtattttc catcgccgcg ctccttaaaa accttgaatt ttattgatgc gcgggcattt
    27301 tatgtggtaa tcagatagag cgctactctc taaaaagagg ggaaaagggt cagatcagct
    27361 tgagctttac agcaatgata agtgcttgag agcgggtgcg tacccccagc ttcccgcaga
    27421 tgttttccat gtggaatttg gcagtggttg cactgatttt taatcgagcg gcagtttctg
    27481 gcatattcat tccatctgcc atggcgcgta gaacatccaa ttctcttttc gtcaaaccca
    27541 gagaactgcc tgtttgttcc cagtctgaag gggtggtttt aaaggcttca ggcgaaaaaa
    27601 ccattttccc ccgatggatc atattaatgg tttcggccag atcgtcttta agtgtggttt
    27661 ttaatacata tcctgcagca ccacttcgaa gcatctcaaa cagcatgtct tgatcctgca
    27721 aactggtgag gataagaatt tttgagttgg gcattttggc ctgcaaagct tttgttgctt
    27781 caaggccatt gataccaggc atcaggacat ccatcaaaac cacatcagga tgcagttttt
    27841 cgcataccca aatagcttcc tccccatttt ttgcatgtcc aaccagttta atatctgttg
    27901 tctgatttaa gatggtcgta acgattcgat ggatgtaatc atgatcatcc actaaaacga
    27961 cgcgaattgt gtttgggctc atggtgaaat tataaatgaa gtctctaaaa attctcctta
    28021 cagaataata atcctaacgc ttgaaccagc gcccgagagt aataagcaaa gaataaatag
    28081 aaaaaacctt aaccccagcg tccttgtggt caaaagcggg acatactcag ttaccaaaag
    28141 ccgcaatctg ggttacttat tgtgtgtctc acgcccatga tcacttcact cactgcgagt
    28201 ggaaaacctt gaaaatcggc tcttttaata gctataatct ctcgttttca aatttgtttc
    28261 gttcacaaca aggagttcca tgaccaacct cgaatccatc gcacgaaaaa ttaccagcat
    28321 tctttttgcc cagcaatccc ttgcttcggc agggttcatc gccgccgcca ccctgaattc
    28381 cattgttggc aaggaactca gcggacattc tgactgggcg ggcgtcccct ctgcaaccta
    28441 tcttttggcg ggtgcatttg ccgcctttat gtggggctat gtttacgaca tgatcggccg
    28501 ccgcaacggc ttgaccatcg gtctcggaat cggcgcagtt ggttcaggaa ttgcttttta
    28561 tgccatcgcg atccattcat ttgctatttt tctcggtggc atggtgctga tgggcattgc
    28621 gaatgccgct gttcagcttg gtcgttttgc cgctgcagaa gtgaatcctc ccgagcatcg
    28681 tggacgtgcc atctcgaacg tggtgattgg cggaacggtt ggttcagtga tcgggccgtt
    28741 tgttgctggg ccagcaggag acctgatcgg ttcatggggt attgatgaat tggcgggtgc
    28801 gtatctggtc agccttgcct tgttcgtcat cgctgcgatc gtcgtgttca tcggccttcg
    28861 ccccgacccg cgtgagatcg gaaaacaagt cgccgaaaaa taccccgata ccatcgttcg
    28921 ctcgcgtgtg gtacgcagca tgatgcagat cttcagccag cccgccgccc tcgtggcact
    28981 caccagtatg gtgctcggac aaatggtgat ggtgttggtc atggtcatca cctctctgca
    29041 catgcgcgga catcatcatg acctgaccga tatttcactt gtcatttcgt cacacacttt
    29101 tggcatgtat gccttttcca tcatttcagg ccgtctttca gatcaatggg ggcgtggacc
    29161 tgtgatcatc actggttcgc tcacactcgt cattgcctgt atcgctgcca ccatttcgcc
    29221 cgatgtgctg ccacttggtg tcgccttgtt tttgcttggt ctcggttgga atttctgctt
    29281 tgtcggcggt tccaccctgc ttgccgatca actttctcct gatgagcgag cgcggacaca
    29341 aggtttcaac gatctgcttg ttggtctcgc ttctgccatt ggaagtctca gcagtggatt
    29401 catcttcgcc gcacttgggt ataacccgat ggcattgatc agcgctattt ttgcactcat
    29461 ccccctgttg ctcgcttctt tctggacctt gaaacgggcg cgaaaacccc gggcaattgc
    29521 ctagcctcag cagttcgtct taatcaaaaa agtagagcga ggtgttatct cgctctactt
    29581 tttttctact tctttcctga tttggactta ctgttcatcc cagtttttgg tccacttgga
    29641 tttggttgtg agttgcctgg catctttggt ttaggtgagg catttcccaa ccccacccga
    29701 tttccaacca aagtgaatgc tgcattgagg cttttctcag cggttgcttc atccagaccg
    29761 gtgcgcttcg aaatctcttt aaccatgccg ctattgtgta acaggcttga gtctacctgg
    29821 cctgcgccca tttgtcccag catttcatca agatcaaatg tattcgagtc gcggcccgag
    29881 gtggggtggt gggcgagtag tttatgtgcc acgaaagaga caatgaccat tgcgatctct
    29941 ggcgaaatgt tgaccttctt ggccagcggt tcaatgaatg gctgcagcat tgccatgatc
    30001 gggtcattgg cttgctgacc gccgcccatg atcccttcaa gggcacccat catcccgccc
    30061 aagccctgag tttgcggctg gctcggcgtg ttttgagctt gttgtgcgcc aagcaaacta
    30121 ccgaccaaac ctgccacatc acctgcatcc actccatctg agaggttgat gccaccttga
    30181 gattgcccgc cgccaagcaa actaccaacc agactggtca ttgggtcggc ttcttgagcc
    30241 gaattgccct gttggcgtga gttaataagg accttgagga tatcttccat tgacatgttg
    30301 ttcttctcct ttttataaca aaaagcgcag cgcgatttgc gctacgcttg caaaaacggc
    30361 tgagttattc cgagtcgccg cctgagggtg acttgctttc agtggacgcg ctttctttcg
    30421 gtgcgctgtc tttttccttc ttgctggtct tctttgtact tgaagtactc gaagattctc
    30481 ccgaaggcga cttgtgatcg gttgcataga accccgaacc tttaaagatg atcttggtgg
    30541 gggtaatcac cttcttgagc gccttcttac ggcattccgg acaggttttg agaggggtgt
    30601 ccgtgaagga ttgatgacgt tcgaactgca caccacaagc ttcacatcgg taggtataaa
    30661 ctggcatttg tttttctcct tgctgttctt tctcgacaac gccgcggatt atagcccgat
    30721 tgatagggtg gggcaagcct tgaatgcctc atgttataat tttctaacaa ttaatggcca
    30781 gaccttgatg gtcatggcct tttgcggagg gcgctgtgtt aaagatcgag atcgtctatt
    30841 gtgcggtttg acattacacc aaccgggccg tgagcctggc cgacgattta ttgaagaact
    30901 ttgagcatgt cattgaatcg gtggcgttat ttccctcgga cggtggcagg ttcgaggtca
    30961 gtgtgaacgg gcagttgatc tattcaaaat tacaggcgaa acgtcatgca gaagcaggcg
    31021 aggtcgtcaa tctcatctca aaaatcgtat cataaagcag gataataatc ccgctcaacg
    31081 aagaggataa agaatggcaa aaaaaggcag agtgttttcg ggggcgcgcc caacggggcg
    31141 tcagcatctt ggcaattatc tgggtgcgat caagaattat gtcgccatgc aggcggatta
    31201 tgagtgtgtg tactgtattg tggatgtcca cgcgttgacg acggtggaga ccacgcagga
    31261 attaaagcag aacacctttg agatggcgtt ggattggctg gcagcgggca ttcgccccga
    31321 agactcgatt ctgttcgtgc aatcgcatgt gcctgaggtg atggaattac acacctacct
    31381 ttcgatggtt accccgctag gtaagttgac cgacctgccg accttcaaag agaaggttcg
    31441 ccagcagcct gaaaatatca actatgggtt ggtgggctac ccggtgctga tgaccgccga
    31501 tattgttttg tacaaaacgg atgttgttcc cgtaggcatt gaccaggctc cgcacattga
    31561 atttgcacgc gagatcgtgc gctcgttcaa ttaccgctac aagaccaagg cgttggttga
    31621 gccgcaggtg aagcatacgg aagtgttgaa ggtggtgggc ctggatggca aggacaagat
    31681 gggcaagagt ttgaataacc acatcgaact ggcttccacc cctgaagaga cggtcaagcg
    31741 tgttcgtgaa atggtgaccg accccgcccg acagcgcaag accgatcctg gcaaccctga
    31801 tgtgtgcaat gtgttcacca tgcacaagct tttctccgca caggaggaag tggatatgat
    31861 caacacagag tgccgtaagg caggcattgg ctgtgtggat tgcaaactgc gctttgccgg
    31921 caacctcaac aagcaccttg agccattccg cgccaaacgc gccgaacttg cttcgaatcc
    31981 gaatcatatt caggagattt tgaacgacgg tgccaaacgt gcccgcacca ttgcccaaca
    32041 gacgatggcc gaagtacgcg aagcaatgca gttgccgtaa tttactgaac aagccataaa
    32101 gtgagaagta aaaagtataa aatgcttttg cttctcactt tttatatcca atttaatcca
    32161 tgcgtttatt aattcaacga gtttcccagg ccagtgtcac cgtagaagag cagaccattt
    32221 ccaaaattgg aaagggtctg ctgattttgt tgggaattgg ccatggcgac ggtgaagagc
    32281 aggccgcctt tctggccgaa aaaacagcaa acctgcgtat ctttgaagat gaccagggca
    32341 agaccaacct ttcagtgctg gatgtgaagg gggaggccat cgttgtttct caattcacct
    32401 tgtatgctga tgcccgaaaa ggccgccgcc catcttttat tgacgcggca ttgcccgatg
    32461 ttgctgctcc gctggtggag cgttttgttg aactgttgcg cagccatggt gtgccgaccc
    32521 aaacgggcag gtttggcgct cacatggatg ttgaaattca taacgacggt cccgttacca
    32581 tctggttgga aaaatgacca ttaaacctaa tttttttcaa aaattcgtcc atcgcttttt
    32641 aatgatgcgc tgggtctcgg cttttttggc agtcagtctg catcgtttgg atgcgatcgt
    32701 gctgaagctt tcaagaggcc gccacacggt taccgagctg gtcggcctgc cgatcatcca
    32761 gatcaccacc attggggcaa agagtggaga gccgcgtacc atgccgctgg taagtttgtt
    32821 tgacggtgag aagatcgcgt tgatcggctc aaactttggc cgtcagcaca accctggctg
    32881 gtattacaac ctgaaggcca acccgcaatg cgtggtgcag acccatggtc gtgagcaaaa
    32941 gttcattgcc cgtcaggctg taggcgaaga acgcgaaaaa tactggcaga tggcggtttc
    33001 atattacaaa ggctacgagc tttacaaagt ccgcgctgca catcgccaaa tccctgtgat
    33061 ggttttggaa cccatgaaat aattgtcaag attagaattt catgataaag ttgttttgta
    33121 ggatgcaagt agcttttcgt tgtggaagta tgaagatgat ggcccaaagc attgcctttg
    33181 agtcgttttg tttaaccgcc cgatgaaaga catactgcgt caccttattt tttttatttt
    33241 gctaggaggc aaaaaatgtc tgatcgtgta atcggtacgg ttaaatggtt caacggcacc
    33301 aagggctttg gtttcatcga gcgcgaaggc ggccccgatg tctttgttca cttctccgct
    33361 attcgcggcg aagggttcaa gaacctgcag gaaggccaga aggtggaatt caccatcgaa
    33421 aaaggcccca agggtttgca ggctgctgac gtcgttgtcc tgtaattcgt aaccaaccaa
    33481 aaagagtctc gctgaaaagc gggactcttt ttttgtttga tgggggaatg ggcgcattgc
    33541 gctcgacgag tagattgatt ctaattaaca agcagaatct tcccgcttgg atttttctga
    33601 ttaaatttgc ggcgccgcac ccgcttcttt cggtgaccat aaactggcat ccatttcgac
    33661 cgccattgcc cccgctgaca gcatatcggc ggcgtctttt tccgtccaca caccgcctgc
    33721 gccaatgacg ggaattccgc tcatggcagc ggcgcggacc gtatttagcg cttgtggaaa
    33781 tatggagggt ccgtataccc tgcccgtggt caatttgttt ttgtcacgcg aagcgaccaa
    33841 tgccccgcgt ggacaggcaa tactgatcgc tacggcgcct ttttcgatca cttttgaccc
    33901 cagcgaaagc acctgttcca tgggtagaga gaagatcaat ggtacttcac ccaggcacat
    33961 atcaagcgta agcaggagaa tatcatttga aagcagaggg gcaaatccca gctcaacggc
    34021 cattacgttt tcgatgcctt cgagcatgcg caccatttga gcggtctctt cggggcggtc
    34081 agccatgaga tgggggatga tgggtaggtc ggcgcggttc caacgggcac tgtgtttttt
    34141 gatcacggct ggtagcccgg ggttgggcag gccggtgtgt agcaggaatc cgccggggaa
    34201 ttcgatcaaa gctggttggg gagtgggcag gcgcgggcgc agagagatcg gattcgtgat
    34261 gaaggctccg aacctgtcaa atggaatccc tgcacggggg tcgggtgaga atcccagtga
    34321 tcccgccgca ttgatgattg gtttgctaaa atagatgtca cgtttcattt tgttttgtta
    34381 tctatgtcat tgcgagggcg atgcacttcg cccgaagcaa tcttatcttc gttggagatt
    34441 gcttcgtcgc aaagagcaaa gacgctcctc gcaatgacaa tgaggagtat ttatgcaacc
    34501 cttgcaagga attcgtgtgc tggatttaag ccgtgtgttg gctggcccat attgtacgat
    34561 ggttctcggt gacctgggtg cagaagtgat caaagtagag ccgcctgaag gggatgagac
    34621 acgcgcctgg gggccgccgt ttgctgaggg ggagagtgcg tattatttgt gcgtcaatcg
    34681 taacaagcgt gatattgtca tcaacttgaa gaccgacgaa ggcaaaaagg ttttgcgtga
    34741 tctggctttg caaagcgatg tgctggtgga aaatttccga ccgggtacgc tggaaaaatt
    34801 cggcttggat tttgcgactt tgagtcaatc gaatccgaag ttgatctatt gttcgatcac
    34861 tggtttcggg cagacggggc caatgcgtga taagcccggg tacgatttta tgatccaggc
    34921 tctgggcggg ttgatgagca tcaccgggga gcctgagggc gagccgatga agactggggt
    34981 ggcggtggtg gacctgtttg cggggcagaa cgcggtcatt gccattttgg ctgccttgca
    35041 agcgcggacc ttgactgggc gaggtcagca gttggatatt gccctgttcg attctcaatt
    35101 gggctggctg gcgaatgtgg ccagtaatta tttgatctca ggtaaattgc caaagcggca
    35161 tggcaatgcg cacccgaaca ttgtgccata tcaaagtttt caagccagtg atggatggtt
    35221 tgccattgcg gtgggaaatg accgtcaatt tgggcgattg tgtgaagtca ttggcagatt
    35281 ggatttggca aatgatgaac ggtttgcgaa aaattcggcg cgtgtgcaaa accgcgagga
    35341 gttgatcggt ttgctgaaat ccattttttt gatgcggcct gtgagtgaat ggttgtcggc
    35401 tttggatgag gcggagattc cctgcgggcc gatcaacacc tttgagcagg tcttttccat
    35461 gccacaggtg caggagcggg agatgctggt gaaaatggat cacccaacta ttggcgaatt
    35521 accgctggtt ggctcgccgt tgaaattcag cgatacaccc gtgcagtatc gcctacctcc
    35581 gcctttgatg ggggagcata cggaggaagt gttgagggag ttgagagata gaggaatgaa
    35641 aggagaatag gccatacact aatgtgctgg caaaatttaa aaatgaaatg gctgatccct
    35701 tttttcctga ttctgcttgg cgtatccatt ttttgctgcg gatttatgta tgacgccgta
    35761 tttgctggga taccctacca agacccaacc cccgagttgg ttgctaaata caattttcat
    35821 tccgaggttg cttctgtgat ccgttggggt ggggcaggga cttgtgcttt cggcgttgtg
    35881 gttgcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatct acgggtcgtt
    36001 tctttttttg atgtgataca tcgtcaatag cggactccac tgcttgcgga ggatggtcaa
    36061 gccgcgtttg gtgagatgct catccaacac cttcagcccg aaatcggtgt cgggatgtcc
    36121 ccagcggaaa aggaaatcga aggctttgat atccaccccg cgcggttctt cgaggaagag
    36181 actgccccca ggtttcagta cgcggacgat ctcgtcaatg gctttgcgcc aatcggggat
    36241 gtggtgaagg acgccgaaaa tgatgacaac atccttgctg gtagtggaaa attgactcag
    36301 atcggtggca tcttgcaaca aaaattcgaa ttgcggatat ttcacccgtg caagttcgat
    36361 ctgctcctcc atgacatcga ggccgatgta gctcttcggg ttgagttggt taagcaggtc
    36421 tgcgccatag cctgaaccgc agccgatttc aagcacatcc ttgttttgca gatccaatcc
    36481 catgtgacgg aagactggca tttcaaagaa gcgttgcccc aacttgcggg gaagtgattg
    36541 catggcacgg aattcggggg tggatagttt cataaaaaat ctccccttcc ttgatgaaag
    36601 aaaggggaga aaccttacgc tgtttccatg aaacccatgg cttgcagcat ctcttcgttg
    36661 ctggcaaact tgaattcctc aagtccgcgt tttctggcct cttctgcttc cacagcttcg
    36721 agccgtttga tatcttcctt caagatgatg ggtttgccga gatttttcat tttttcagct
    36781 acaacttcgg cgctggcatt ggcgggttgt ttggtctgca aatattgcca gacggccttg
    36841 gcggcatttg tcccatcttt gcgggcataa ccgaccaaac cctcactggc tttacgagac
    36901 cagccgccga cgaaaatccc ttccactgcg gattcgaagg tgagattatc tacagggaaa
    36961 cgcggtgaat tatttttgat gaactcgttc cattctgtgg gcaggccaaa ggattcatcc
    37021 actttatcgc cgatggcaaa gatgaccgta tccacatcga gggtgcgttt ggcgcctgtg
    37081 cctttggcgc tggtcttgcc gtctctttcg gtgaggatgt tatcctccac ttcaagtttg
    37141 gtcagcaagc cgttctcccc atacatggca gtgggggagg cgaggaagtc aaagcggaat
    37201 ttgccgtggg cggttttggg gtctgctttc gcgagagaat ccaatatttt ttggcgacca
    37261 gtttcaaggt cctgtttgac cgcttcaacg gcagggcgga cccgttccat ttcacggtta
    37321 aaatcatcaa ggtcaagata tgagatcaaa tgcttcatct cttccttggt gaaattcacc
    37381 tcggctgggc cgcgccttac aatggctgtc acctcatcca cattttgcac gtggagcaga
    37441 tgacgggcaa tatccaccat cacattccct gcaccaatga tggcacagcg ttttccaaag
    37501 tggaattttt tctggctgaa gggcgggagc ttgttgtatg aaaatacaac gtctttggcg
    37561 tgatacacac cttcaaggtt ttcacctggc aagcccagtg attttgtgcc ttgtgcccct
    37621 gcagtgacga gcacggcatc gaagccaagc gcgcgtaatt catcgagggt taagtctcca
    37681 ttggcgccga tggtgacatt gccatagtaa tcaatattgg caaggtcaag cacctgacgg
    37741 aattgcttgc gcagcccttc tttcattgta tgtttgtctg ggtaaattcc atactcggcg
    37801 agaccgcctg ccttcacatc gcggttgaaa acagccacat gcgcgcccag tgtggccagc
    37861 tcacgggccc caaataaacc ggcaggcccc gcgccaatta cggctacaaa aaatttatta
    37921 ctcacttgca cgtacctcct gaatttgaca aaaacagtcg tattatattt gaaaaaattc
    37981 ccatccacaa gcagtctcat cctatgctaa gattacagag tgctgagcaa ttggaatcgt
    38041 gctaaaggag atcacatggc tgaagataac aaaaaagacc ccgcgcaaaa aatgcgatac
    38101 ctgctcaatg gaggaaaacc ctccccagtg gcgcgccttc ccaaaaagga tggcggtgtg
    38161 gcatcgttcc ttccaaaatt gaagaaggat gatgccccaa cggaaacccc cgccgctgtg
    38221 gaagtggatg ttgctcccaa caaggatcaa cccgaagcag cgcatcagac tccgccgaaa
    38281 cctgctccat ttcaaggggc attatcgagc ctgaagggaa tcaaactcaa catcggccca
    38341 gctttctgga ccattgcgag tgtcatctca atgaccgtca atgtggtttt gctgatcgtt
    38401 ctactggccc tgcttccaaa cctcaccaaa ttaaacctcg gcagcgcgtt ggatattggt
    38461 aattatctgc tgggcggcct gtacagcaat tttgaaaaaa tggatcgcgc ccacatcacc
    38521 acatccattc cagttgaaac caccattcct gtgaagttcg atttgcaact caatcaacag
    38581 accaatgtgg tcttaagtca ggatgtgacg attaccaatg ccctggtaac ggtcaacaca
    38641 ggcggtttga acatcacccg cgcattaacc accatcgtct tgccgcaagg aacaaacctg
    38701 cccgttgtgc taaacttggt cgttccagtg gatacgactg tgcctgtggt attgaatgtg
    38761 cctgtggata tcccgctttc gcaaactcaa ttgcacgaac cctttgcagg cttgcaggat
    38821 gtggtcaggc cgttttattg catgctcagc cccaatgccc ttggcattga tggacagcca
    38881 gtttgtaaat aacgccaggt atcctggtgg attaggctga acaacagtgt tcagaataga
    38941 ttaaacctat cggagacaga tgacaaatca aatcacgcag gtcacactga agaacggcat
    39001 gaaggtcatg ttaaaagaga tccataccgc gcccatcatc tcttcatgga tctggtatcg
    39061 tgtgggctcc agagatgaac ccacggggaa gacaggcatc tctcattgga ccgaacatat
    39121 gcaattcaag ggaacgaaga aattcccagg caatgtattg gataaagcca tttcgcgcga
    39181 aggaggacgt tggaacgcat ccacttctcg cgattcaacg cgctatttcg aaaccatgcc
    39241 cgccgctaaa atcgatcttg ccttgcgcct ggaatctgac cgcatgaaga acagcctgtt
    39301 tgatgaaaag gaggtcgcct ccgaacgtac ggtcatcatg tcggagcggg aaggcttgga
    39361 gaatgaacca actttcagat taagtgaggc cgtgcaacag accgcctttc atgtgcatcc
    39421 ctaccaccac gagatcatcg gcgatatggc tgacctgcgc acgatcaccc gtgatgacct
    39481 gtacgaacac tatcgcaggt attacgtccc gaacaattcc attttgacca ttgcaggcga
    39541 tttccaaacg aaagcaatgt tgaagcgcat tcaggaattg tttgagtcca ttccgatggg
    39601 caaaacgcct ccacgcctaa atcgccctga acctgagccg aaaggggagg tgcgcctcac
    39661 tgttgaaggg ccaggtgaaa ctacatttat tcaggtggcc tatcgcttcc caaatgcagc
    39721 gcaccccgat tatttcccat tgcaggtgct tgacagcctg cttggcggtg ccagcggcct
    39781 ttcctataaa acatcccgcc tgtaccgtgc cttggtggat aaggaattcg cagtggatgt
    39841 atccagctgg ccggaagcga ccattgatcc gtttttgtat cgcatcattg tcaccaacca
    39901 tcccaaacga aaaacagacc agacacttgc cgcccttgat gccgagatac aaaaattgca
    39961 ggaaaaacgc gtatcgaaag atgaaattac ccgtgctgta aaacaggcac gcgccgtttt
    40021 tgcgtatggg agcgaaaata tcaccaatca agctttctgg atggggtatg ccgagatctt
    40081 ttcatcctat cggtggttta ccagccattt gagcaacctt gccaaagtga ctccgcaaga
    40141 tgtgcagcgt gtggcgcaga aatatttcca gcctgccaat cgggtcatcg gcatctacac
    40201 acccaatgga cggaggagca aatgaacaaa cctgtttctg taaaaaagaa atcctccctg
    40261 cctgggcctg aggatattca ccgcgagata ctttcaaacg gcatcacagt gttggcgcgt
    40321 tccaacttca acagtccatc catcagcatg gctgggtacc tgcccgcggg cgcgatcttt
    40381 gagaccgacg ataaactcgg gttggcagat tttgtctctg cttcgttgat gcgtggcaca
    40441 cagaccaaaa gtttcgatgc gatctataac gagctcgaat ctgtcggggc tgggttgggc
    40501 tttgattccg gggttcacaa tgcaagtttc ggtggccgtt ccttggtgga tgacctgcca
    40561 ttaatgttga agttgttgtc tgagtcgctg cgcacacctg tttttcctaa gaacgaaatt
    40621 gagaaattgc gagcacaaat tctgaccggc cttgccatcc gcgaacaaga tacatctgat
    40681 atggctgcaa tggcctttga ccgcttgtta ttcgatggac atccttatag caggccgtca
    40741 gatggatttg tggaaacgat tcaaagcatc acccaaaaag acctggtcaa ctttcatcgc
    40801 gatcactatg gacccagagg catggtcatt gcaatcgtcg gtgcgatcga cccgaagaag
    40861 gctgttgcag cggtggagcg tgttctcgga gggtggcagg ttccaggtca ggttgtgccg
    40921 ccgcttcttc ctccatacaa gccgatcaag aaaacggtca ggcatcacca tacacttgca
    40981 aacaaatccc agtctgattt ggtgatcggt atgattggtc caaagcgaaa ggatccggag
    41041 ttttattctg cttcgctggg taattcggtt ttgggtcaat ttggcatgat ggggcgcatt
    41101 ggcgaatcgg tgcgtgaaaa atcagggctg gcctaccatg catcatcaag cctgagtgcg
    41161 ggagtggggc ctggtacctg ggaggtgaat gccggagtga gcccaaagaa cttgaagaaa
    41221 gcaattgatt tgatcgaaga agagttgcgt aaatttgtaa agagcggtgt gacaaaacgg
    41281 gaacttgagg acagccaggc aaactacatt gggagattgc cgctttctgt tgagtcaaat
    41341 ggtggtatag ccaatgcgtt gttaaatatt gagcgttaca acctcggctt ggattattat
    41401 cagcgctatg aagggctggt gcgtggtgtt acgcgcgatc aggtgttggc tacggcacaa
    41461 aaatatattg acccgaatcg cctggtgatt tcaacagctg ggccgtaatt gatttcgtat
    41521 atgaagcttg ctactggagt tgatttaatt gaagtcagcc gtgtccgtga ggcgattgag
    41581 cgccacgggg agcgttttct tgcgcggatc tttacggaaa ttgagcaacg cgaatgtggc
    41641 ggaaaatttc catcacttgc tgcgcgtttt gctgtgaagg aagcaacagc caaggccttg
    41701 gggactggca ttggtgatgt cagctggttg gatatcgaag tgcggggcga tgagaatcgt
    41761 gctccgcact tgtatttaca tggggcaggg gaagagttgg cccaaaagct gggattggtg
    41821 acctggtctg ttagcatcag tcataccgag acacaagcca ttgcttttgt tgttgccatt
    41881 gggtaatgaa aacatcccgc gaccagtatt cgcgggatgt tcgtaaggac ggttgagatg
    41941 tgggaatggc gaaggacttg gagtccacca catctcaatt catttttggt cctcaacgcc
    42001 gatccaaagg tgcccaggca tgacgggttt gtaggggcgg ataattaatt tcccaagttc
    42061 aacttgtctt tgcccgtttt ccatcatgct agggtggaca aggcataagt cgggaatgtg
    42121 gccgaatttc ttgcgataga aatccatggc tttttgaatt ttaatactca aagtggttcg
    42181 tggatcattg tcaaaccata acattccagt atgcatgatg aagtcctttc tcaaattgcg
    42241 tttgtgcgtc aatccagtgc gggagctaaa aacctgagcc agttctgtcc ctcacgcatt
    42301 gcgaattgtg agcccgttgc caatgtgcca aggatgtcgt tggatgcgtt cgcaaccaaa
    42361 tgcgcgtctt gcaagttctg gatattgcca aacaggcgtg tgcgaaagaa tccaagccat
    42421 gtttcaatgt gatgtgcgcg gcttgcgttg agagtaacaa ttggccagac tttacgggag
    42481 gttccccgta acaaaagcca gcgcaaacat tgttcagtct cctgatcgag tttggttaga
    42541 aactcaagat cgtcaatcaa aagcaaaaaa gtgcgttcat cgtttttgtt ttggtgtgcc
    42601 cattcaacaa gagactggat aagttcttgt gcgccattgt cttgcgtggt ataaactccc
    42661 atattgctct cacgatcttg aaagttattc cactcttctg catgtgatgt gacaacacag
    42721 tattgaatat ctgaaggaga gtggagtaat tcggcagctt gagcgatggt ttgcaagagt
    42781 ccggtctttc cacaagcttt gtcaccaaca atcaagattg gccctggaat cggatcgtca
    42841 agattaagta aaactggcaa tccatcttct gcaatgccta aaaaaagagc ttcttcaggc
    42901 aaaggagtaa tgtctgcaag gacactgttt aatgttggca ggataatttc aggcatgcga
    42961 gaagtgtgac cagactgttc ttcttttgcc agttcagtca atgcattgat cattaaggtg
    43021 aagtgattgg aattgtccat tattagaacg cctgttctaa taatacaacc aaagagtttg
    43081 aatgtcaagg cgcaaagttg ctgtccaatt tggaaataat gaaagtgcaa taatcttgac
    43141 ggtatgcgtt gttttggtat aatccgcctg cttaatcact tgccgacgta gctcaatcgg
    43201 cagagcaccc gccttgtaag cgggcggtta cg
//
LOCUS       DAOQJC010000016        42893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_381_length_42893_cov_9.459779, whole genome shotgun sequence.
ACCESSION   DAOQJC010000016 DAOQJC010000000
VERSION     DAOQJC010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 42893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42893
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_381_length_42893_cov_9.459779"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2150)
                     /locus_tag="PLE39_03875"
     CDS             complement(<1..2150)
                     /locus_tag="PLE39_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNE67491.1"
                     /translation="MYKTRSMHLFPKVLPSIALTVLLFIPFLSGSVEAVRALGGGAEP
                     NQSDGDTLYYFAEGNRIPLTPSLRWISVKFSESLSDQTAALENFAPMVGSLDQARPIL
                     FGDITLLPLNANMNVQSVLAGVNAMRADTAKFSQVYPVFQTADAEMALTDEFIVTFPS
                     GFRMEDIHNVNAFHGVELVEPVLGQENTFVLRVASGVDVLAMANLYQESGVALHSAPN
                     FVRILTSKPAVGQIPPSIAPMTDVNDPLYANQWYLNNTQQFGPETKNDADIDAPEAWA
                     LTTGSSSIIIAVIDEGVDLMHEDYKSNLVVGYDATGLGSKGAPVADDAHGTNVSGLIA
                     ASANNGLGVAGVCPQCRIMPVRVAYGDKYGYWVTDDATIANGIAWAYQNGASILNNSW
                     GGGLESTAINTAIANARSFGRGGKGSVVVFAAGNNNMTVVYPANLENVITVSASNLCD
                     QRKQPMDDACNGNEYWWGSSYGNSVDVSAPGVLLYSTDITGAAGYDTGNYYAEMNGTS
                     GAAPIVSGVAGLILSLNPNLTADEVQNILQSTADDVNGDGYDVDMGYGRVNAYNAALA
                     ANASSITFTDVPNTYWAYDFIERLYNSGVTRGCSSDPLSYCPLAAVTRAQMAIFILRG
                     MHGDTYVPPTASGTVFTDVTAETFGAAWIEQFALEGITSGCGSGVFCPNDNVTRAQMA
                     IFLLKGKYGASYVPPAATGIFADVPVGSFAADWI"
     gene            complement(2360..4522)
                     /locus_tag="PLE39_03880"
     CDS             complement(2360..4522)
                     /locus_tag="PLE39_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNE67492.1"
                     /translation="MKNSSHFFRFALVVIMLVAIFANTPVASASNSAQTQKAGAQTGF
                     VSDLYNTGDPVLTDIWVDAVNGNDANDGSSPASAFRTLTAAWNSIPTTNPLPQGRRIN
                     LQPGTYTESMIPNYWENRYGTFTAPIFIRGNGSSRGEVVLQGTISMYNVNYIYFENLS
                     MIFNGDVFHCELCNHILLRNTVLNGGMFAHETIKANQSQHLYVENSDISGSYENAIDY
                     VAVQYGHVVNNQIHDADDWCAYFKGGSAYLRFEANVVYNCGTGGFTAGQGTGFQYMVS
                     PWLQYETYDIKVVNNVIHDTDGAGLGVNGGYNILMAYNTLYRVGSVSHVIEVVFGGRT
                     CDGQAGDPGREMCQQNLNLGGWGTTAVDNGSNSVNIPDRNVFIYNNIVYNPAGFQSQW
                     QHFAIYDSRANPAGTNAPNPAHTDTNLNIRGNVIWNGDSGMVLGIEGNSDACIASNPT
                     CNETQLRADNAINTVEPLLVNPSAGDFHINGTWAAGVTTYDIPDFVWDIGGVPGGDNS
                     NAVPVDFEGVTRTGSNPPGAFFGGGSDTIFADVTSSYWAWPFIERLYNAGITGGCNTS
                     PLMYCPESSVTRAQMAIFILRGMHGSAYVPPAASGTVFADVPLGAFAVDWIEQLAAEG
                     VTAGCGGGNYCPDASITRAQMAIFLLRGEHGSAYSPPAGTGTVFSDVPAGAFAVDWIE
                     QLAAEGITSGCGGGNYCPDANVTRAEMAVFLVRAFSLP"
     gene            complement(4818..6578)
                     /locus_tag="PLE39_03885"
     CDS             complement(4818..6578)
                     /locus_tag="PLE39_03885"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNE67493.1"
                     /translation="MNRKMLCVLIVLFSFGAVHVVSARPALALIDSPPSLGFAEIASG
                     LSSPVSITNAGDSSGRLFITLQGGQVVIYDGSQILPTPFLDVSSLIRNGSEQGLLSVA
                     FHPNYENNGYFYVYYTRLSDAALVIARYEVSADPNIADAGSASILLTIPHAEASNHNG
                     GQLQFGPDGYLYIGTGDGGTGGAYSQDGLSLLGKMLRINVNGAAPYSIPADNPFVSDP
                     SVADEIWALGLRNPWRFSFDSLTGDLIIADVGQNAWEEVNVQPASSNGGQNYGWPCYE
                     GMHSNGDTSTCTIGTITSPVVEYEHGASDTNGCSISGGYRYRGSEFQSLKGVYLYGDF
                     CTGRIWGAEPNNGSWIAVELADTDYGVTTFGENENGELYVADFFGGVIYKVQATTFTD
                     VPLDHPQYAYIEALYDAGYTAGCSSSPLMYCPDTILDRAQSAVFMLRGQMGTGYTPPP
                     APWDTFTDDWTGFEWAQPWAEGMYQEGLTGGCQTSPLMYCPATQLPRVEASVFGLRMK
                     YGVNYIPPAATGTLFADFPATDPSYWAIDWAEQAYLDGLLPACGTDTVSGKPMFCPNE
                     LVDRGWGAFLIVNAKNLPLP"
     gene            complement(6737..8692)
                     /locus_tag="PLE39_03890"
     CDS             complement(6737..8692)
                     /locus_tag="PLE39_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNE67494.1"
                     /translation="MLQKTLVVFARKPMYLLMILGIIFSTFGTAQAQEFNGSIIPTGL
                     TQIQVDDEVFASLGQKGEARVIINLRDPVSIAAPSNERTSAIARIQGSVLGRLSSQDF
                     RVLRQYVNVPALAGHLTSSGLDILMSDPNVTSIDMDQMGGGHLSESVTALGADIVHSN
                     GYTGLGVTVAVLDTGIDTDHPDLSDDLVAQHCFTDSQCKPGSTAEGTSAEDENGHGTN
                     VSGVISSGGVVSSVGFAPDASIVAVRVLDANSSGWVSDWLAGLDWIRTNQGTLGVDII
                     NMSLGTFALYSGHCDADNSSVANVVAQLNSLGIVIFASTGNQGSSTQMAMPACLSGVV
                     AVGATFDSDLGREPDSGTYQDIFGGNWPACANDTTNLQTITCFTNSNSVMDIVAPGAR
                     ITAPGMGGGTSTYSGTSQASPTAAGIAALMLQASPGLSPATIEATLKSTGTSVTDAKN
                     GLSFPLINALAAVNAVNTTFTDVPSTYWAYDFIERLYAAGITSGCSTSPMMYCPLSPV
                     TRAQMAIFILRGEHGSAYTPPTATGTVFSDVTTSTFGAAWIEQFASEGITSGCGNGMY
                     CPDANVTRAQMAIFLLRGKYGSAYVPPVATGVFTDVPVGSFAADWIEQLAAEGITSGC
                     GGGNYCPNAEVTRDQMAVFLVRTFNLP"
     gene            complement(8822..11101)
                     /locus_tag="PLE39_03895"
     CDS             complement(8822..11101)
                     /locus_tag="PLE39_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNE67495.1"
                     /translation="MKNSKTVRLFSKLLTVIALAAMVFSTFVSGNVSAAQAKGAQPSI
                     SSQAADGSFYYYADGKRIELNPSLSWVAVRFANDDLGAQSAAVDNSSALLGSLDQARR
                     ITPSSLALLPLKAGVTAATIRDAVKALRADTAGFALVNPVFQTSDAEMAISDEFVVTF
                     PAGMGMDEINAINAANGVELVDAILGQDNTFVLRVTGGADLLEMANKYQESGIAIHSA
                     PNFIRIVQKPAPAPSSPKPDFAPMAGTNDTYYPYQWHLNNIQQYGAGTTLDADIDAPE
                     AWDLTTGSSSIIIAVLDEGVDLSHEDYSSKLVPGYDATGGGSSGGPWGNDAHGTNVAG
                     LAASGSNNSLGVAGVCQACKIMPVRIAYSDANGNWVTTDATIANAITWAYQNGADILN
                     NSWGGGSEATVINTAFSNAKTLGRGGKGSVIVISAGNSNDPTVSYPAYLSTAIAVAAS
                     NLCDQRKAPINNLCNGYEYWWGTNYGSALDISSPGVWLFSTDITGAAGYASGNYFDQM
                     NGTSGAAPIVSGVAGLVLSVNPNLTSDEVQSILQSTADDVNGGGWDAEMGYGRVNAYN
                     AVVAAGAGSTTFSDVPATYWAYDFIERLYNAGITSGCSTSPMMYCPTASVTRAQMAIF
                     ILRGIHGSAYVPPAATGTVFSDVSTSTFGAAWIEQFAAEGITSGCGGGKFCPDDVVTR
                     AQMAIFLLKGEHGSSFVPPVATGIFPDVPVGSFAADWIEQLYNEGITSGCGGGNYCPN
                     SFVLRDQMAVFLVRAFNLP"
     gene            complement(11171..11836)
                     /locus_tag="PLE39_03900"
     CDS             complement(11171..11836)
                     /locus_tag="PLE39_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67496.1"
                     /translation="MSKAFFRVILKAKGLLVFLCFILLTGCAGGTGAPANKTEFQPLS
                     SDSTSFYYYVSENRVPLTLSTKWIAVQFANEDPSAQASALKGSIVDANGQAEQLTRYG
                     VTLLPVQAGTTFETLVAGINALRSDSTFFQVNPVFQSGDTGMIVTDEFIVTFPAGMSM
                     DEINAVNASYGVEVGDPILGQENTFVLRATTATKMDTLSLANLYQESGVAMFAAPNFV
                     RVR"
     gene            complement(12062..13708)
                     /locus_tag="PLE39_03905"
     CDS             complement(12062..13708)
                     /locus_tag="PLE39_03905"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M35 family metallo-endopeptidase"
                     /protein_id="HNE67497.1"
                     /translation="MKIGNARKLFYVAMILMLALAAFGSWDVSAASAQGSLGGATVRL
                     SVDRASFGTGDRVLVKVNISNPTNSQIKILKWLTPAMGVEAPIFSITRDGKAVAYLGK
                     LAKRMAPTDADYITLPPGGSISSEVDLAEYYDLSASGNYSVQYNIASANLYRWDQSQS
                     TDLLVSASVDLFVEGRANRVIQRVDPQAVVGTNTFNGCSSSQQTDLVNARNAASTYAA
                     DDVAYFAANKQGERYVTWFGVLDSARYNLVKSHFTNVQNAVDTASPMHFDCTCTDAGV
                     YAYVYPDSPYEIYLCGAFWSAPTTGTDSKAGTLIHEITHFTVVAGTDDYAYGQTAAKA
                     LAISNPAQAVMNADSHEYFSENNPPLEVSSGTFTDVPSTYWAYNFIERLYNAGITGGC
                     SSSPMMYCPESSVTRAQMAVFILRGEHGSAYVPPAATGTVFGDVSTSTFAAAWIEQFA
                     SEGITSGCGGGNYCPDSNVTRAQMAIFLLKGKYGSAYVPPVATGIFSDVPVGSFAADW
                     IEQLAAEGITSGCGGGNYCPSANVTRAEMAVFLVRAFNLP"
     gene            complement(13750..14313)
                     /locus_tag="PLE39_03910"
     CDS             complement(13750..14313)
                     /locus_tag="PLE39_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67498.1"
                     /translation="MDTKRQNLKGMILVGGAVLVILIAVFVFRNFNTQGASAKAPMSA
                     TVTLSIDQGSFAASDDVLVHVTITNPNDTPIRILKWFTPINGLERSLFTITRNGETVP
                     YIGRMLKRAEPTEADYITLEAGGSITADVNLAEYYALSATDDYEVMYDVTSLQLYIEK
                     EIGELTNGRLSSTPLKMFITGRAAPAH"
     gene            14463..15863
                     /locus_tag="PLE39_03915"
     CDS             14463..15863
                     /locus_tag="PLE39_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67499.1"
                     /translation="MRKIFSGIGAFLQTPVGIILTTNVIWHIVVLVAKIPFNYFASAV
                     ILGLDIFAAFYLRDKLIYFFSQFVLPIQNPKHRREIAARVSNFESGRRGPALFVKNGR
                     VILHEGEAGKRGAGVIVLDTASALVLRTDTEIKDTVGPGIKFTSGNEYIAGSVDLRSQ
                     WQFIGPLASDQPFLNPASNPSKASNERRQQTSGMTRDGFEVSPTISIKFSIKRPVEKK
                     PTESGVTSQYGFDAEAVRNAITREVIQLGTTENTKVRMEWNKLPAHLVVNIWREYIRK
                     FKLSDLFTSSGVSGLQTIEDMINKRVKQSNVMALDDTGSPTGEWLESLEFKQLESRGL
                     EIQEVSIHNVLFEQEIENQVISQWSTEWMNLAKKEEGQLKEKEALIETASRDEGSKSF
                     ARIASRQFSGKPTMPQQNPFKTLQLLIQPLKEFILFESSANTDVEKELRKLDDIWKWL
                     LDNNPDGPRNQQGGQP"
     gene            15860..17461
                     /locus_tag="PLE39_03920"
     CDS             15860..17461
                     /locus_tag="PLE39_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67500.1"
                     /translation="MNKARRDELNTLLFSLTDEGVDFRNRWRSIAQIVVWLTLSGISM
                     LSAVFFTQATWQTAVVIGMSLVKYAPLMAVVYSLARKMASLYLDDIFELHDEELASQF
                     LEEVTFGYGLDKITINEGRVVEKDETSPIILIGGPGAIQVNLDSVALLEKVNGEPEVI
                     YPRKDAWKLGRFERIREIGKFDEVGKREYAIINLRDQFISGLTVKSRTKDGIPIEAQD
                     IKVIFSILRKPKPENPEAPDSSYLFDEEAVKALVYNQTIITPVPSTPSAVSFPWDTSA
                     IPLITSELENLITSHTLSEILVTIGQKEIDNTSNNEQTITQMRVELTGQQTTLGSKKA
                     ASAPSFKSRSRITAQFFEKPFQDKAAAVGIYIEWIDIGTWQLPSSLILDKHKEAWNLA
                     RENAKKAASVERLRKRFETAEIVSLIENVVIRNYERPVSGSYRSSYSGSKEKEADKEW
                     DAFIAANPDLKGQLNPRESRPKDSTATATALEILKAFRKELLAGKELIEKENRPFEEK
                     QADLLKIEKALFDISHLTHHWIRKS"
     gene            17461..18387
                     /locus_tag="PLE39_03925"
     CDS             17461..18387
                     /locus_tag="PLE39_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HNE67501.1"
                     /translation="MKKNNLTLSLEVSRALNHGAPLVALESTVITHGLPHPQNLELAR
                     DMEKQIREAGATPATVALLDGEIRIGLSDLELVRLSEADSTLKVSHRDFATAIVKKAD
                     GGTTVAGTMFVANMAGIKVFATGGIGGVHKESPFDISTDLRSLAEIPTIVVCAGAKAI
                     LDLPATLEYLETMGVPVIGYRTDEFPAFYSRESGLGVSARLDTPKEIADFAKAHWNLG
                     MKSGILVTNPIPEAEAISKSEMEPMIAQASAEAIAQGIHGQKLTPFLLGRISELTKGK
                     SLKANLALLLNNARLAAEIAKEMSGKKREWAI"
     gene            18394..18786
                     /locus_tag="PLE39_03930"
     CDS             18394..18786
                     /locus_tag="PLE39_03930"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE67502.1"
                     /translation="MKAEFTLTATFNASADKIYQAFLSTQGHTLMTGSPAKVDGKVNG
                     EFTAWDGYIWGTFLELEENKKIVQAWHTAEFPEDAADSHVEILLEEANGKTKLTLIHT
                     HIPKGQAEGYQQGWEDFYFKPMREFFGG"
     gene            complement(18921..19415)
                     /locus_tag="PLE39_03935"
     CDS             complement(18921..19415)
                     /locus_tag="PLE39_03935"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNE67503.1"
                     /translation="MTDKAFQDYYPEYFAHCYGCGSVNELGHQIKSFWDSEESVCYFQ
                     PKPYHTAIPGYVYGGLLASLIDCHGTGTAAAAGYRAENRAMDTEPPLRYLTASLHVDY
                     LKPTPMGPVLEVRANVKEVKGRKVVVEEWIVVNGVVTVRGEVVAVQVPEKLVLELLEG
                     KDGG"
     gene            complement(19518..20324)
                     /locus_tag="PLE39_03940"
     CDS             complement(19518..20324)
                     /locus_tag="PLE39_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67504.1"
                     /translation="MDIHGGIIAAAILVAVAALFVVRASVRVMQNARKMSFYSLRRMH
                     NANALRLFLFALVLFGCSFWLPFFGEPIVYDFFPPSPTASLTPTITLTPTITLTPTIT
                     PIPSETPTPAVSDTPTLTFTPFLPVAIEALFSGPVTPNPDALFTPLQFSTEFDGLNPV
                     DPKTVFELPIPKMYGGFDYNNTLPGVQWTALWYRDGQLVCYETTPWNGGTGGIGGYTE
                     CDDPIGGWQAGTYEVQIFMGYDWKVVGQFIVHSELATPTPTSTPTMTPTP"
     gene            20385..21182
                     /locus_tag="PLE39_03945"
     CDS             20385..21182
                     /locus_tag="PLE39_03945"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HNE67505.1"
                     /translation="MKTEIVLLKLGGSLITDKDIPYTPRLDKLKELALEIKTVLDSTP
                     NLLLILGHGSGSFGHVAAKKHGTRNGVQTAEQWQGFAEVRFQAAELNRYVMEALYNAG
                     VPAISFPPSSSLVSKNRKVIAHNSLTIRKSFDFHILPVVYGDVAFDEALGGTILSTED
                     VFTFLVEQFSPTRILLAGIEAGVWEDFPARTRLVKQIQLSDYEKMRAGIGGSVSTDVT
                     GGMKAKVEEMLDLIQKNPNLTVQIFSAEENGLLTRALRGENVGTLLK"
     gene            21283..23283
                     /locus_tag="PLE39_03950"
     CDS             21283..23283
                     /locus_tag="PLE39_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0035999 - tetrahydrofolate interconversion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNE67506.1"
                     /translation="MATKKSAKAKAAPKARKIVKKAVVKKAAPKAKAAFTPTKLKLVR
                     PVPSDIDIAQAAKLKPILQVAEELGVRPNELELFGPYKAKIKLEILERLKNKPNGKYI
                     DVTAITPTPLGEGKTTTTVGLSQALGAHLGKKVITAIRQPSMGPTFGIKGGAAGGGYS
                     QIIPMEDFNLHLTGDIHAITASHNLVAAALDVRVMHEKEQDDEKLFNALCPPNKKGER
                     KFSPTMLRRLAKLGIKKTNPNDLTPEERSRFARLDIDEKTITWRRVVDINDRMLREIE
                     VGRGKDEAGHAHMSGYDITVASEIMAVLALTTGLEDMRDRFGRMVVATNKRGEAVTVE
                     DLGVAGAVTVLMKDAIKPNLMQTLEGTPATVHAGPFANIAHGQSSIIADMIALKLVGK
                     DGFVVTESGFGADIGMEKFFNIKCRYSGLIPQVVVMVATVRALKMHGGGPKVVAGKPL
                     APEYTDENLELLKAGLPNLERHIQNALKFGVNVVVAVNSFATDTKAEVEMIREAALKM
                     GAMDAVVSTHWADGGAGAKALAEAVVKAASKPSNFQFLYPLENPIKSKIEDIASKIYR
                     ADGVDYTPEAEEQIERYTRLGFDKLPICMAKTHLSFSTDATKKGAPTGFRITVREIRA
                     SVGAGFLYPILGDMRTMPGLPTRPSFYDVDLDLETGKVLGLF"
     gene            complement(23370..23957)
                     /locus_tag="PLE39_03955"
     CDS             complement(23370..23957)
                     /locus_tag="PLE39_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67507.1"
                     /translation="MKKTKTILLAMTVLLALWVSACGGSAAPATQAAVPPVVESAPTQ
                     APVVESAPVQEQPLATVQSFAPACQAAASCAAPAVTDTVASDRYCVEKIPYQNILVDD
                     GVTFEVLDPANLICSDNGTMVDGKHVVECHGTEAWSSQVKFSNTACSAAGLQTGTGQC
                     QEGLGYDAANNCCAPLSAGDAGSITITVNMGECPN"
     gene            24062..25423
                     /locus_tag="PLE39_03960"
     CDS             24062..25423
                     /locus_tag="PLE39_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNE67508.1"
                     /translation="MNLSFLSLTDQRDLIKSREINASELADACYRQIERLNPTLNAFI
                     TVIPPEQLSAQEETQSGLLQEIPIAVKDLYNTKGIRTTAGTRFFADHIPQEDAYVVQK
                     IKKAGAQIIGKTNTHEIALGVTNNNPHYGACRNPWDITRTPGGSSGGSAVAVSTGMAM
                     AALGTDTGGSIRIPASLCGVVGLKPTFGRVSLRGILPLSWNLDHAGPITRKVEDAALM
                     LQVMGGYDELDPASMKTLPGDYYSHLRDGIKDRKFALATGSFIEESDPEVLEAVRKTA
                     QVLEEQGAVITETNVDFLKEAAVANALMTQADGAAFHRERLKEHPDWFGADVRQRLEI
                     GAAFTSSEYILARRTQAEVKRQLELLFEEYDGLLLPTTPITAPVLEGENALERARQLT
                     RFTAPFNLTGLPALTIPCGFTSEGLPIGLQIVSRAWNEAGVLRTGYAFQQATEWHTRM
                     PSI"
     gene            25501..26088
                     /locus_tag="PLE39_03965"
     CDS             25501..26088
                     /locus_tag="PLE39_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67509.1"
                     /translation="MLKATDLVLVALIPTPRDMEIARLLGWYRIPLRTAPKVVAVDYL
                     AFYQPAAFGERSGQIECIAQVKGHELTTRGELLKDERNHPRAKEEYYKIQLGGLEKLK
                     EPIKTDKWKRLTFLYSTGDYILNAKTLNDLVVEGGERQLLWKNLRERAENEQLYKTDL
                     PEADIPPEVLMALLGIKELQEPYEAGAPNAQKENE"
     gene            26256..27668
                     /gene="hydA"
                     /locus_tag="PLE39_03970"
     CDS             26256..27668
                     /gene="hydA"
                     /locus_tag="PLE39_03970"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HNE67510.1"
                     /translation="MNTLIKNGTLVTASDTFAADILIEGETIAQIGRDLQHPNAEVID
                     ATDRLIMPGGIDPHVHLDLPMFGTVSSDDHYTGHKAAAFGGTTTVMDFVVLENEGFDH
                     SVDLWMKKAEKAAIDYSFHMNLTNFNDKIAKEIPSLMKMGIQTLKVFTAYNGRLRIDD
                     GSIFKALRIAKENGMLMMAHCENGDVIEALIPEALNAGHTTPEWHAYTRPAWGAVEAT
                     LRMAAMAEQADSPVYIVHMNAGGEVDMLKYARENGVKVMGETCPQYLFFTIDHLYRPD
                     GAKWICSPPMRTEQDNARLWEGLSGGVLQTVGTDHCPFYFDGTSPIVYEGSEIAIPGK
                     ELGKDDFTKIPNGLPGIQDRMPIMWTYGVRAGYITPNQFVAYNCTNPAKIFGLYPRKG
                     ALIPGADADIVIWDPEKQVKYGVAHSHQRTDYNLYEGWELAGYPEKVFLRGKLIVDGE
                     EWKGKRGDGQFLKRGAGEIL"
     gene            27710..28087
                     /locus_tag="PLE39_03975"
     CDS             27710..28087
                     /locus_tag="PLE39_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67511.1"
                     /translation="MPAKKPIIHCDALVVHCMDYRLQKFIQPWITVRFGYDNFDIISL
                     AGSVHDYEMVLKYVQLAVLIHSIETVCLINHEDCRAYGRDGTYKRHKHDLKDAQAKIK
                     KLFPHLHVETFYLHLDGTFEPIS"
     gene            28100..28462
                     /locus_tag="PLE39_03980"
     CDS             28100..28462
                     /locus_tag="PLE39_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67512.1"
                     /translation="MTHNCQAIVVHCMDYRLQSHINQWLESNFEKGTYDRAVMAGGVY
                     DVYDVIRQVDIAARLHGISKVILINHEDCGMYGPGGTEERHEEDLDKAEDKIRRLFPH
                     LEVDTFYLNLDGTFEKNS"
     gene            complement(28464..29069)
                     /locus_tag="PLE39_03985"
     CDS             complement(28464..29069)
                     /locus_tag="PLE39_03985"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNE67513.1"
                     /translation="MNLTEEYITQKLQAAMRSSTDADGYAEMAVHPDTRLKCAAVLVP
                     LTQVNGEWHLLFTRRTEIVQSHKGQVSFPGGACDEGETTPEQTALREAEEEIGLRPAD
                     VRVIGRLSNLITISSFRVSPIVGVIPWPYTFKVAGVEVARVFTIPLLWLSDRNNYWEF
                     FVRDSERSLIAYHPYDGELLWGATARMTVTFLKTIETLAKK"
     gene            complement(29078..29620)
                     /locus_tag="PLE39_03990"
     CDS             complement(29078..29620)
                     /locus_tag="PLE39_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67514.1"
                     /translation="MFRRRGQQRMFRGGRREGPPVLQQANQLMLDGEYEEASSLFADL
                     GQRAEERFPHRAPFLFIEAGRAAILGGQTKTGVAHLRRGLTLFASQGRVHRMRMVGER
                     AIDELQARGLKAEAEEISALLGSNSLPDASGEKEIPAPTKRPVLPTHCPSCGAAVRPD
                     EVEWLDEVTAECAYCGSPVR"
     gene            complement(29676..30506)
                     /locus_tag="PLE39_03995"
     CDS             complement(29676..30506)
                     /locus_tag="PLE39_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HNE67515.1"
                     /translation="MIDSTITHDNDLIQLVGLTHKHFIFSLKAGAELHTHRGMIKHDD
                     LIGKPWGSQVFSHTGAPFFLLQPSIADILNDLPRATQILYPKDIGYILIQMGVAPGQR
                     VMEAGTGSGSMTIALATAVGANGRVISYEQKQDTQTLAKKNLERVGLASRVEFKLRNI
                     QEGFDETGVDAFFLDVQNPYDYIPQVRAALKPGGFFCTLIPTFNQVEKTLIALRKNQF
                     AFVEVCELMLRHYKADSARIRPTDRMVAHTGFLIFGRRIEPNTDPRGKELEQEIGMEE
                     "
     gene            complement(30511..31764)
                     /locus_tag="PLE39_04000"
     CDS             complement(30511..31764)
                     /locus_tag="PLE39_04000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNE67516.1"
                     /translation="MQTKNTHWPSILILIVLGTSVLFVFLFIAATGISSIIDLFSESG
                     DPAGEMISTFAFGFELLVLLVCMWFVFQKAQSREQADLQFKFPFANWQILAVLGLIGL
                     SVMLGAAVIYTEVIALAWVVLPFLTLLVIVPPIWIFFGLGTKGIDFGSRWRVFSILGL
                     GMTLAPVTMIVLELIILFVIIIAGTVFLALSQPDLLQEVAAVGRILQNETDQDVILNL
                     VAPYLSSPAVIATLIGYIGFIVPLIEELLKPLGVWLFARKFTSPAQGFAMGMLSGGAF
                     ALVESLNASGNGSAEWVVVVSVRAGTSLLHMVASGLVGWGIVSAFQERRYLRLPAAYF
                     CAVAIHGLWNSCAIGMGISSVGESLGRPEWLAAYAPSMLGGLVVLGVGMFVVLLAANR
                     KLSSVAVISPTPPAETRAETEEKVK"
     gene            complement(31764..33008)
                     /locus_tag="PLE39_04005"
     CDS             complement(31764..33008)
                     /locus_tag="PLE39_04005"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE67517.1"
                     /translation="MKRIVFFLIISIVSMIGLSACAAEAETAPVTVAVQPTSLPDVAT
                     PISESSCSAIVVAPTPTPGVDSYFPPATADDFSYGPADAPVTIIEYCDFQSPGCGAMA
                     LVFAEFLRSQENVRFVFRPVPLLDVMDKSDKATMAAIAAGEQGQFWNMYDLLYLRQQE
                     WINMEPRAFDDWVVREAVILGLDGEKLTAAMNAPQTEASMRSMYEAAKKLRIPAVPLV
                     LINNSPQSANILNYRNLSETVGLIALGQKQFSECPPFDIDPVKQYIATVKTEKGDIVI
                     QLFPDKAPLAVNSFVFLARQGWFSGVTFHRVLPGFVAQAGDPSGTGRGNPGYFFNNEF
                     SDLKFDKPGVVGMANSGLNMNGSQFFITFGPASHLDGGYTIFGQVISGLDVAEQLTPR
                     DPDQAVIPAPGDKIISVEIEEK"
     gene            complement(33105..34379)
                     /locus_tag="PLE39_04010"
     CDS             complement(33105..34379)
                     /locus_tag="PLE39_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67518.1"
                     /translation="MFQRIIRGLEITLILFIIAAITGHGNPSLTDKIEKVRAFTRQIE
                     FNYMAWMGNAALLKLRSASVGVPYTLNYESRKQIVTEYLRVTQSILENEYQLQQIYAD
                     AAITDKELASEELRAELDEQYARQAKLAPLAEATLQEQVTIILSEIGLAAVGQPVPNV
                     WYHSTPLPYALIVSPRDRIEQIANISINTEITLDEQAALESRVDAGLNTSSLVVPIGG
                     VGVYPTMVMRSTNLPWVLNTISHEWIHNYLTLRPLGFLYANTPELRTMNETTASIAGN
                     EIGALVIERFYPELKPASRLSPGLISAPFDHPEPGDFLRPPFDFRAEMHETRITVDAM
                     LAEGQIEEAEAYMEARRLLFLQNGYLLRKINQAYFAFYGAYADSPGGAAGEDPVGPAV
                     RALREQSESLADFVNTISWMTNFEQLQEAIKK"
     gene            complement(34472..35914)
                     /locus_tag="PLE39_04015"
     CDS             complement(34472..35914)
                     /locus_tag="PLE39_04015"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE67519.1"
                     /translation="MQAVPSEFLAIDRKERANRPFFDLDLRPAEVRTCDFDDVVIEFD
                     PERAMVEAARCIHCPDPAPCMVACPTANDIPSAMWLIEQGRFVEAAGIYHKTSSLPEV
                     CGRVCPHEALCQGSCVLNKHHQPVLCGELEAFTVDYERRTQGYVIPLGIPSGKKVAVI
                     GAGPAGLGCAEVLVQKGHDVTIFESKPAPGGLLVYGIPNFKLSKEVWQEKWGEFERAG
                     VKFVPNTYIGKDKTIDGLFEEGFEAVFIGVGSEVDAKMEDTPGTELPGVYEATDFLIR
                     GNVERDQLPEHMRAPLEVGKRVVVIGGGDTASDCLRTAIRLGSKDVTCLYRRTEKEMP
                     GGKKDRKMAREEGAKYRFLTQPVKFIAGSDGKLAAVECIEMKLGEPDAKGRRKPVPVE
                     GSNFSVAADTAILALGYWPDPIIGKTTPELEVHDWGLITVKDKARGNTSRPGVFSGGD
                     CVTGPDLVVTAMVGGRKAALAIDEYLKNKK"
     gene            36226..37482
                     /locus_tag="PLE39_04020"
     CDS             36226..37482
                     /locus_tag="PLE39_04020"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE67520.1"
                     /translation="MKQFFSKITRRTWVIIGVLAVVVLAIVFFSTRSSGEPLYQTTTV
                     ERGNLVATVGATGSVRARQSAVLIWQTTGIVKDIYMEVGNPVKRDEILASLDKNSLNQ
                     SVILAESDLAAAEKALADLLNSDTARAQALRTVDAAEEAYKKAYDYRLELNGLITITE
                     TYLVRGVPKVRTYKGYADAETIADADEKLALAEAQLNDARRNLDRLQNGVDPAELAAA
                     EGRVAAAQSTMNMAHIIAPFDGVITQSEPSVGDQVTAGTVAFRIDDVSHMLVDLQVSE
                     VDINNVAIGQPAVLTFDAILGKEYHGEVVEVGQAGDTVQGVVSFTVTVELTDADELVK
                     PGMTAAVNVVIEEQKDMLLIPNRAVRLVDGERVVYLLVDGQPVQVKIKLGSSSDTMSV
                     LAEGDIKEGDLIILNPPSNNGGPFGG"
     gene            37484..38179
                     /locus_tag="PLE39_04025"
     CDS             37484..38179
                     /locus_tag="PLE39_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE67521.1"
                     /translation="MDLVIEARDLRKTYQMGEIIVEALRGVSFTIERGEVVSIMGPSG
                     SGKSTMMNTLGCLDRPTSGDYILDGESVAEMNDDQLASVRNRKVGFVFQSFNLLSRLT
                     ALGNVELPLRYAGITEGRRERAQAALEAVGLKDRMLHRPYELSGGQQQRVAVARSLVN
                     NPAIIMADEPTGNLDSKVGKEIMSLLLNLNKDRGTTLIIVTHDPTIAEQTQRVIRLRD
                     GELDPASEIKRKG"
     gene            38184..39443
                     /locus_tag="PLE39_04030"
     CDS             38184..39443
                     /locus_tag="PLE39_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE67522.1"
                     /translation="MSFSQALLEAIESLNGNKMRSGLTVLGIVIGVAAVIAMLAVGNG
                     AQASITGSISSIGTNLLFVFKGSPDGPPGPGGGGRGSGNNDRPLTLSDAEAIADPLAA
                     PSVEIVAPAIQGNGTITFAGENTTTTISGVTPNYAPVRNLEIAEGEFINEEHILGRMS
                     VVVLGPETANAIFGHADGIVGETIRIEGQPFRVIGVLVAKGGGAFGSEDNSAYIPFTT
                     AQARLIKRTSLDEIDVLFVQATTAESVPQAADEISNILRQRHRTPIGDDDFTVFTQQD
                     FLQTFETITGVLTIFLGGIAGISLLVGGIGIMNIMLVSVTERTREIGLRKALGARKKD
                     ILLQFLTESSLLSLIGGVIGVIFGWLIAFAVGQVATATGNNFVPIVGTDAILLSTSFS
                     AIIGLFFGIYPASRAANLEPVEALRYE"
     gene            39452..40168
                     /locus_tag="PLE39_04035"
     CDS             39452..40168
                     /locus_tag="PLE39_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE67523.1"
                     /translation="MTKTFQIKRSILTAALIVVLLQACMQVRVDAPLALPTPQDIETV
                     AVESPAATATASTTPTTAPATATETLPSATPQPEVVIHAVKGNLFIRRGPDMAFNPIG
                     VLYKDTSTPVLARDVLSNWVQIVIPDSDQKGWVSLQTQYSQVDGDLRTLPELAPTDWP
                     LPAYLRNCTHHQMYILPAEVILLSSLDHPDNMVWLNPGTYTVYDMDVAGEPEVTTVEV
                     REGVTVEILDDGAGEHRKCP"
     gene            40352..41848
                     /locus_tag="PLE39_04040"
     CDS             40352..41848
                     /locus_tag="PLE39_04040"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HNE67524.1"
                     /translation="MSEKLNQLKEILGEVNDLYHAASVLEWDQNVSMPPGGSEARGQQ
                     LSTLGKIAHEKSTSDEVGKLLDELKKEYTDPNTDDGALIRVASRNYDKSTRVPASFVA
                     RQALTTSKAFDAWVEAKGKSDFALFQPHLERVLELVREYVSFFPPADHPYDTLLDDYE
                     PGMKTAEVQGIFEKLRPQQVKLLKAIKSSKQVKDDFLQKKYNEKKVWAFSEAIITKFG
                     YDWNRGRQDKAPHPFQTTFSVNDARITNRYEPNNPMATLFSAMHEAGHAFYELGSNPA
                     YERTPLAGGTSLAVHESQSRLWENLVGRSLPFWEHFYPDLKKAFPSQLDGVSLKAFYK
                     GINKVESSFIRVNADEATYNLHVMLRLELEVGMVDGSFAVKDLPEIWNTKMQEYLGIV
                     PPNNALGVLQDVHWSNGLMGYFSTYALGNLVSAQLWEKINKDIKDLDDQIRKGKFDTL
                     LHWLQEKIHTFGHKYDPQDIVQKVTGSRIDSAAYVRYLNTKYSDIYGL"
     gene            41925..42857
                     /locus_tag="PLE39_04045"
     CDS             41925..42857
                     /locus_tag="PLE39_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67525.1"
                     /translation="MKKHPLLLASLLTLITACTQLTGPAPTQTPAHSLPTVVALTGQA
                     AFATASALVPTSAPPTETLVPTETPVPTTPLPSATPTVAAGFNDFAEIHFLSPGPMSS
                     LVSPINLQVILIAGESEIVQVDLLGEDGRVLERVLERVQRNLAGNYRSFELSFEIRAV
                     SEKGYIRISSKDDHGRIQALNTMPVLLYSVGVNQVNPLGNMIYERIIYEGLEDGDEIF
                     GGTINLKGRFWPFNEQPAFLELMLPDGEVISSRVLDFNGIETQEFETTLPYKVTEPTL
                     ARLSIRQDNPDISISDPDLKKYVYIYTMEVMLYP"
BASE COUNT        11127 a        11815 c        10523 g         9428 t
ORIGIN      
        1 tcaatccaat cggctgcgaa ggaaccaacg gggacatctg caaatatgcc cgtggcagca
       61 ggcggcacat aggacgcgcc atatttgcct ttgagcagga aaatggccat ttgagcacga
      121 gtcacattat cattcggaca aaataccccg gaaccacatc cggaagtgat gccttccaat
      181 gcaaattgtt cgatccaggc ggcgccaaag gtttcggctg tgacatcggt aaagaccgta
      241 ccgctggcgg taggcggaac atacgtgtcg ccatgcatac cgcgcaaaat gaaaatggcc
      301 atctgcgcac gagtcactgc cgccagcgga cagtatgaca acgggtcaga actgcatcca
      361 cgtgtgacgc ctgagttgta caaacgctcg ataaaatcat aggcccagta ggtgttcgga
      421 acatcggtaa atgtaatgga gctggcgttg gcagccaggg cggcgttgta cgcattcacg
      481 cgcccgtaac ccatatccac atcgtagcca tcgccattga catcgtctgc agtgctttgg
      541 agaatattct gaacttcatc tgccgtcagg ttgggattaa gcgaaaggat aagccccgct
      601 acaccggaaa cgatgggagc cgcccctgac gttccattca tttcagcgta atagttccct
      661 gtatcgtatc cagctgcacc agtgatatct gttgagtaca gcaagacgcc cggtgccgaa
      721 acatccactg aattcccata gctgctcccc caccaatatt catttccatt gcaggcatca
      781 tccatcggct gtttacgctg gtcgcataaa ttcgaggccg ataccgtaat tacgttttca
      841 agattggctg gatacacaac ggtcatatta ttgtttccag cggcgaagac tactacagaa
      901 cctttgccgc cacgcccaaa ggaccttgca ttcgcaattg cggtattgat cgctgtagat
      961 tccaacccgc cgccccagct attattcaaa atggaagcgc cgttttgata agcccaggca
     1021 ataccatttg caatggtggc gtcatcagtg acccaatatc cgtatttatc accataggcc
     1081 acgcggacgg gcatgatgcg gcattgtgga cataccccag ccacaccaag tccattattt
     1141 gcgctggcag ctattaaacc gctgacattg gttccatggg cgtcatcggc aacaggcgca
     1201 cccttgctgc caaggccggt ggcatcatac ccaacaacaa gattggattt gtaatcttca
     1261 tgcattaaat ccacgccttc atcgatcacg gcgatgatga tggatgaact tccagtggtc
     1321 agtgcccatg cttctggagc atcaatatcg gcatcgtttt ttgtctctgg tccaaattgc
     1381 tgggtgttgt tcaaatacca ttgatttgcg tataaagggt cgttcacatc tgtcatcggt
     1441 gcaatggagg gaggaatctg cccaacggct ggtttgcttg ttaagatgcg gacaaaattc
     1501 ggcgcggaat gaagcgcgac tccactttcc tgatacaggt tcgccatcgc aagcacatcc
     1561 acaccactgg caacccgcaa cacaaaagta ttttcctggc ccaaaacagg ctccaccaat
     1621 tcaacgccat gaaaagcatt gacattatga atatcttcca tgcgaaagcc agatgggaat
     1681 gtgacgataa attcatcggt cagagccatc tcggcatctg cggtttgaaa gacgggatag
     1741 acttgagaaa actttgccgt atctgcacgc atggcattga cacccgccag caccgactgc
     1801 acattcatat ttgcgttcaa gggcaacagg gtgatatcgc caaaaagaat ggggcgggcc
     1861 tgatccagtg acccgaccat cggagcgaaa ttttcgagtg ccgcagtttg atccgacagg
     1921 gattcagaaa acttcacaga aatccaacgg agggatggag tcaatggaat gcggtttcct
     1981 tctgcaaaat aatacagagt atcgccatcg ctttgattcg gttccgcgcc cccgcccaat
     2041 gcgcgaaccg cctcaacgct ccctgacaaa aagggaatga acaatagaac cgtcagggcg
     2101 atcgagggca gaacttttgg aaacaaatgc attgacctgg ttttatacat atcaacctcc
     2161 ttgtgttgtg aatcgggttt cgatcacaac ccttgtttgc aaactgattt tgattttgtt
     2221 tgtttgcagc aaggtgaatt cactgaaatt ttttcttctt acaatttttt taggttcacc
     2281 tactgaaaaa ataaacggct tcttcaatcc cattttcgaa ctggattgaa gaagccgtgt
     2341 tttcatctca gaatttctcc tatggcaggc tgaaagccct caccaaaaag actgccattt
     2401 cagcgcgcgt cacgtttgca tcaggacagt agttaccgcc gccgcaacca ctggtgatgc
     2461 cttcggctgc caattgctcg atccaatcca cggcgaaggc accagcgggg acatcgctga
     2521 aaaccgtgcc cgtaccagca ggaggactgt aggcgctgcc atgttcgccg cgcagcagga
     2581 agatagccat ctgcgcacgt gtgatggatg catcgggaca atagttgccg ccaccgcaac
     2641 ctgcggtcac cccctccgct gcaagctgct cgatccaatc aacagcaaat gcgccgagag
     2701 ggacatctgc aaaaaccgtc ccagaagcag cgggtgggac ataggcactg ccatgcatac
     2761 cgcgcaaaat gaaaatggcc atctgtgcgc gagtgacgga tgattcaggg cagtacatca
     2821 aaggcgaagt gttgcaaccg cctgtgatgc cagcgttata caaacgttca atgaaaggcc
     2881 aggcccaata agaggaggtc acatctgcaa agatggtgtc tgagccgccg ccgaaaaatg
     2941 cccccggggg attgctgcca gtgcgggtta cgccttcaaa atcaacagga actgcattgc
     3001 tgttgtcacc gcctggcacg ccgccgatat cccaaacgaa atcgggaata tcataggtgg
     3061 tgacacccgc cgcccaggtg ccattgatgt gaaaatcacc tgcggatgga ttcaccagca
     3121 agggttcaac cgtattgatg gcattatcgg cacgcagttg ggtttcgtta caagtcgggt
     3181 tgctggcgat gcaggcatcg gaattgcctt cgattcccag caccatgccc gaatcaccgt
     3241 tccagatcac gttgccgcga atattcaaat ttgtatcggt gtgggcgggg tttggagcgt
     3301 tcgtcccagc cgggtttgcg cgtgaatcgt agatggcgaa atgctgccac tggctttgaa
     3361 aacccgccgg gttgtagaca atgttgttat aaatgaatac atttctatcg gggatgttga
     3421 ccgaattgga gccgttatcc actgcagtgg tgccccagcc gccaaggttt aggttttgct
     3481 ggcacatttc ccgaccaggg tcacccgcct gaccatcaca ggtacgacct ccaaaaacca
     3541 cctcgatcac atgagacacg ctgccgaccc gatacagggt gttgtaggcc atcaaaatgt
     3601 tatagccgcc attcacaccc aaacccgcgc catcggtatc gtgaatgaca ttattcacga
     3661 ccttgatatc gtaggtttca tattgaagcc agggagaaac catgtattgg aatcccgtcc
     3721 cctgccctgc tgtaaaaccg cctgtgccgc agttatatac aacatttgcc tcgaaacgca
     3781 ggtaagcgga accgcctttg aaatatgcgc accaatcatc cgcatcgtgg atctgattgt
     3841 taaccacatg cccatactgc acagcgacat agtcaatggc attctcatag gagcccgaaa
     3901 tatcgctgtt ctcaacataa agatgctgcg attggttggc cttgatggtc tcgtgtgcaa
     3961 acataccgcc attcaaaacc gtattccgca acagaatgtg attgcacaac tcacaatgaa
     4021 atacatcgcc attaaaaatc atgctcagat tttcaaagta gatgtaattc acgttataca
     4081 tactgatcgt gccctgcaga acaacctcac cccgtgaaga gccgttgccg cgaataaaga
     4141 tcggagcggt gaaggttcca tagcggtttt cccaataatt ggggatcatg ctttcggtgt
     4201 aggtgcccgg ttgcaggtta attctccgcc cctgtggaag cggattggtt gtgggaatgc
     4261 tgttccatgc agcggtgagc gttcgaaaag cggatgcagg agaggaaccg tcattggcat
     4321 cattgccatt gaccgcatcc acccaaatat cggtcaatac aggatcgccg gtattgtaaa
     4381 gatcagatac aaaccccgtc tgtgcccccg ccttttgtgt ttgtgctgaa ttagaagcgg
     4441 aagcaacagg cgtatttgca aaaatggcaa ccaacatgat caccaccaaa gcaaaacgga
     4501 aaaaatgcga actgtttttc ataccatcct ctttttcatt aatataatta tcggcatgtt
     4561 cgtttgaaat gtatatcggg cataattgtt agaatcaccc acaatattgt cagggacatt
     4621 ttttttgtaa aaacaaaaat gaaatgtcgg ttggcaatgt gataatcaac tgtgaattga
     4681 gataaaacct ttctgtgagc ttacaaaaca acctgccgta acgacacacc tgtctttttt
     4741 gaaatgactc tggccaattc cacaacaggc gttcattcag aagagaatga acgcctgtta
     4801 tttgtttttt tattggctta tggcaacgga agatttttag cgttgacgat caggaatgca
     4861 ccccagccgc ggtccaccaa ttcgttcggg cagaacattg gtttgccgct gaccgtatcg
     4921 gttccacagg cagggagcaa cccatccaaa taagcctgct cggcccaatc aattgcccag
     4981 taacttgggt ctgtggcggg gaaatcggca aataaagtac ctgttgcggc gggcggaatg
     5041 taattgacgc catacttcat tcgcaaaccg aaaacacttg cctccacacg tggaagctgg
     5101 gttgcaggac aatacatcaa tgggctggtc tggcacccgc ctgttaaacc ttcctgatac
     5161 atgccttcag cccacggttg tgcccattca aaaccagtcc aatcatcggt gaaggtatcc
     5221 cagggtgctg ggggtggagt gtagccggtg cccatttgtc cgcgcagcat gaacacagcc
     5281 gattgagcac ggtcaaggat cgtatcaggg cagtacatca gcggactgga actgcatcct
     5341 gcggtatacc ctgcatcgta caaagcctca atataggcat actgggggtg atcaagcggc
     5401 acatcggtga aggtggtggc ttgaacttta tagatcacgc cgccgaagaa atcggcaaca
     5461 tacagttccc cattttcatt ttcaccaaag gtggtgacac cataatcggt atcagccaat
     5521 tccacagcaa tccaagaacc gttgtttggc tctgcgcccc aaatacggcc tgtacaaaaa
     5581 tcgccataca ggtaaacgcc tttcaggctt tggaattcac ttccacgata acgatagccg
     5641 ccactgatgg aacagccgtt ggtatctgat gcgccatgct cgtattccac cacagggctg
     5701 gtgatcgttc cgatggtgca ggtagaggta tctccgttgc tatgcatccc ttcataacag
     5761 ggccagccat aattttgtcc accgttgctg gatgcaggct gcacattcac ttcctcccag
     5821 gcattctgac ccacatccgc gatgatcaaa tcacctgtta aagaatcaaa cgagaagcgc
     5881 caagggttgc gcaaacccaa tgcccaaatc tcatcggcaa ctgacgggtc agacacaaag
     5941 ggattgtcgg cgggaattga gtaaggcgcc gcaccattga cattgatgcg cagcatcttg
     6001 ccgagcaagg acaaaccatc ctgtgaatat gcgccgcctg tgccgccatc accagtgcca
     6061 atgtataaat acccatcggg accaaactgc aactgtccgc cgttatggtt cgaggcttct
     6121 gcatggggga tggtcaacaa gatcgaagcg gaccctgcgt cggctatgtt tggatccgct
     6181 gaaacttcgt aacgcgcgat caccaacgcc gcatccgaca agcgggtgta atagacgtag
     6241 aagtatccat tgttttcata attggggtga aacgccacgc tcagaagccc ctgctcactg
     6301 ccatttcgaa tgagcgagga aacatccaaa aacggtgtgg gcaaaatttg cgagccatca
     6361 tagatgacga cctgcccgcc ttgaagggtg atgaacaatc gtccggaaga atctcctgca
     6421 ttggtgatgg aaactggcga agacaatcca ctggcgattt cagcgaatcc aagcgacggc
     6481 ggcgaatcaa tcaacgccaa agctggccgg gcggaaacga catgcaccgc cccgaaagag
     6541 aacagaacga tcaacacaca cagcattttt cgattcataa acacctccag aatttttgaa
     6601 aaattatgac acaccccttc tcaaaacaaa tcccccattg accctacaaa aaaatctttt
     6661 gcttgcaaat ttgttttgta agaaaaaagc cgcgagacca tggtctcgcg gctccaatga
     6721 ttggatattg aatttactag ggcaggttga aagtgcggac caaaaagacc gccatttggt
     6781 cgcgtgtcac ctctgcgttc ggacagtagt taccgccgcc gcatccgctg gtgatgcctt
     6841 ccgccgcaag ctgctcgatc caatctgcgg caaatgaccc gacgggaaca tcggtgaaaa
     6901 cgcccgtagc cacaggcgga acgtaagccg agccgtattt gccgcgcaac aggaagatcg
     6961 ccatttgagc acgagtcaca ttggcatccg ggcagtacat gccattgcca caacctgaag
     7021 tgatgccttc ggatgcaaat tgttcgatcc atgccgcgcc gaaagtgctg gtggtgacat
     7081 cgctaaagac tgtgcccgtg gcagtcgggg gagtgtaggc actgccatgt tccccgcgca
     7141 aaatgaagat cgccatttga gcacgagtca ccggtgacag cgggcaatac atcattgggg
     7201 aggtactgca tccgctggtg atgcctgcgg catacaaacg ctcaatgaaa tcataagccc
     7261 agtatgtaga tggcacatct gtaaacgtgg tgttgaccgc gttgacggca gccaaagcat
     7321 tgatgagcgg gaagctcaat ccatttttgg catctgtgac agaagtcccc gtgcttttca
     7381 aggttgcttc aatggtggca ggagataagc ccgggctggc ttgaagcatc aacgccgcaa
     7441 tacccgccgc ggtcggcgaa gcttgcgatg tccccgagta tgtggaagta ccgccgccca
     7501 tgccaggtgc agtgatcctt gcgcccggcg caacaatatc catcacggaa ttgctgttgg
     7561 tgaaacaggt gatggtctgc aggttggtgg tgtcattggc acaagcaggc cagtttccac
     7621 caaagatgtc ttgatacgtg cctgaatctg gttccctgcc caggtcgcta tcgaaagtcg
     7681 cacccacggc aacgactcct gagagacagg caggcatggc catttgcgtg gaagaacctt
     7741 gatttcctgt ggaagcaaaa atgacgatcc ccagcgaatt caattgcgca accacattcg
     7801 ccactgaaga attatccgca tcgcaatggc cgctgtacag ggcaaaagtt ccgaggctca
     7861 tattgatgat atcaacgccg agtgtgcctt gatttgtacg gatccaatcc aaccctgcca
     7921 gccagtctga gacccagccg ctgctgtttg catcaagcac ccgcactgca acaatgctgg
     7981 catctggagc aaaaccaacc gaactgacca cacccccaga agaaatgacg ccagagacat
     8041 tggtgccatg tccattttca tcttccgcgc ttgtaccttc ggcagtggac ccgggcttgc
     8101 attgagaatc cgtaaagcaa tgctgcgcca cgagatcatc gctcaaatca gggtgatcgg
     8161 tatcaatgcc ggtatccaac acagcaacgg tcacgcccaa gcccgtatat ccattcgagt
     8221 ggacaatgtc agcgccaagc gcagtcacac tttcagagag gtgcccccca cccatctgat
     8281 ccatgtcaat ggaggtcacg ttcggatcag acatcaatat atccaggccg ctggatgtca
     8341 aatgacctgc caatgctgga acattcacat attgacgaag tacccgaaaa tcttgagaac
     8401 tcaacctgcc caatacactt ccctgaatac gggcgatcgc agatgtccgc tcattggagg
     8461 gagcggctat cgaaacggga tcgcgcaagt tgataatgac ccgcgcctcg cccttctgcc
     8521 ccagactcgc aaagacttca tcatccactt gtatctgggt taatccagtt ggaataatac
     8581 ttccattgaa ttcctgagcc tgagccgtgc caaaggttga aaagataata cccagtatca
     8641 tcaacaaata catgggcttt cgtgcgaaca caaccaaagt cttttggagc atttattcct
     8701 cctatgatgg gttttttatt tcaatttcca gtatataaca aagggaagga ttttcaagac
     8761 caccaacaaa aaagcgtccc tgaaaattca gggacgcttt ttttgctaat acaggtctgg
     8821 cttacggtag gttgaaagcc cttaccagga agacagccat ttgatcgcgc agcacgaatg
     8881 agttcgggca gtagtttcca ccaccgcagc cgctcgtgat gccttcattg tagagttgtt
     8941 cgatccagtc cgcagcgaag gaaccgacgg gaacatccgg gaagataccg gttgcaacag
     9001 gcggaacgaa ggaagatcca tgctcacctt tgagcaggaa gatcgccatc tgagcacggg
     9061 ttacaacatc atccgggcag aatttgccgc cgccacaacc agaggtgatg ccttccgcag
     9121 cgaattgttc gatccacgcg gcgccaaagg tgctggtgga aacatcactg aagacggtac
     9181 cggtagcagc cgggggtaca taggcgctgc catgaatacc gcgcaggatg aagatcgcca
     9241 tctgagcacg ggtcacagaa gctgtcgggc agtacatcat cggggaggtg ctgcatccgc
     9301 tggtaatgcc agcgttgtac aagcgttcga tgaaatcata agcccagtag gtggcaggaa
     9361 catcggagaa agtggtggaa ccagctccag cagccacaac cgcattgtag gcattgacac
     9421 ggccatagcc catctcggca tcccagccgc cgccattgac atcatccgcg gtgctctgga
     9481 gaattgactg cacttcatca gaggtcagat tcgggttcac cgacaacacc aagccagcca
     9541 caccggaaac aatgggagct gcaccggagg taccgttcat ctggtcaaag tagttaccag
     9601 aagcataacc agctgcaccg gtaatatctg ttgagaacag ccaaacaccc ggagaagaaa
     9661 tatccaatgc gctgccgtag ttggtgcccc accagtattc ataaccgttg cacaggttgt
     9721 tgatgggtgc cttgcgttga tcgcaaaggt ttgaagctgc aactgcaatg gcagtgctga
     9781 ggtaggcagg gtaacttact gtgggatcat tggagttacc ggcagaaatc acgatcacag
     9841 agccttttcc accacggccc agggttttgg cattggagaa cgcagtgttg atcacagtcg
     9901 cttccgagcc gccgccccag ctgttgttca aaatatcggc gccattctga taagcccagg
     9961 taatggcatt cgcgatcgtg gcatcggtgg tcacccaatt gccatttgca tcgctgtagg
    10021 caatacggac aggcatgatc ttacaagcct gacatacacc agctacaccc aggctgttgt
    10081 tactgccaga ggcagccaaa ccagccacat ttgtaccgtg ggcatcatta ccccatggtc
    10141 cgccggagct gccgccgccg gtggcatcgt agccagggac caacttggag ctgtagtctt
    10201 cgtgcgaaag gtccacgcct tcatcgagca ctgcaatgat gatggaggaa ctgcctgtgg
    10261 tgaggtccca ggcttccggg gcgtcaatat ccgcatcgag agttgttcca gcaccatact
    10321 gctggatatt gttcaagtgc cactgatagg gatagtaggt atcgttcgtt ccagccatcg
    10381 gagcgaaatc tggcttgggg ctcgaaggag cgggagcagg cttctgaaca atgcggatga
    10441 agttcggggc ggaatgaatt gcgattccgc tttcctgata cttgttcgcc atctcaagca
    10501 ggtcggcccc gccagtgacg cgcaacacaa aggtattatc ctgtcctaaa atggcatcga
    10561 ccaattcaac accgttggcg gcgttgatgg cattgatctc atccattccc atccctgcag
    10621 ggaaggtaac tacaaattca tcagaaatgg ccatttcagc atcggaggtc tggaagaccg
    10681 ggttcaccaa tgcaaaaccg gctgtatccg cacgcaacgc cttgactgca tcacgaattg
    10741 tcgctgcagt tacacctgcc ttgagaggca gcagagccag gcttgagggg gtgattcgac
    10801 gagcctgatc aagggacccc agcaatgcgc tggagttgtc aaccgcggca gactgagcgc
    10861 cgaggtcatc attcgcaaaa cgtacagcca cccaactcaa agacggattc agttcaatgc
    10921 gtttgccatc tgcatagtaa tagaaggaac catctgcagc ctggcttgaa atcgaaggct
    10981 gtgcgccttt tgcctgggcg gcactaacgt tccccgacac aaaggtggaa aataccatgg
    11041 ctgccaaagc aattactgtc agcagctttg aaaataaacg aactgtcttg ctattcttca
    11101 tttgcattct ccttttctaa aacaacaaaa attttaacca aacttgctgg ttaattcaca
    11161 tgggatggga ctatcgaaca cgcacaaaat tgggagcggc aaacattgcc acgccgcttt
    11221 cctgatacag attggcaaga gagagtgtat ccattttggt tgcagtggtt gcccgcagca
    11281 caaaggtatt ttcctgccca aggatgggat cacccacctc tacaccgtag gaagcattaa
    11341 cagcattgat ttcatccatg ctcatccctg caggaaaagt aacgatgaat tcatctgtca
    11401 cgatcattcc cgtatctccc gactgaaaga ccgggtttac ttgaaaaaaa gttgaatcgg
    11461 aacgcaatgc attgattccc gctacaagag tttcaaaggt tgttcccgcc tgcacgggca
    11521 gaagtgtcac tccatatcgg gtcaattgct ccgcctgtcc attcgcatct acgatggaac
    11581 ccttcaaagc tgaagcctgt gcagaaggat cttcattggc aaattgcact gcgatccatt
    11641 ttgtggataa ggttagaggg actcggtttt cgctcacgta gtaataaaaa gaagtgctgt
    11701 ctgaagacaa aggctggaat tcagttttat tggcgggcgc acccgttcct ccagcacatc
    11761 ctgtcagaag aatgaagcac aaaaagacaa gtaacccctt tgccttgaga attacccgaa
    11821 aaaacgcttt actcatggtt gatacccttt gcagcttgaa tccacatatg aaaatctatt
    11881 tcccacaaca ttgaacaatt cagtccgaat ctccttctgg ttggtaattt tagtataagc
    11941 cgaaaaacaa aaatttcaaa tgagaaacca gccccccaaa aggggcatac gagtttttcg
    12001 tacccatatt aaacaaaaag gtgcctctga aaaatcagag gcaccatgaa aacattcttt
    12061 actacggcag gttaaaggca cggacgagga acactgccat ctcagcacga gtgacgtttg
    12121 cgctcgggca gtaattgccg ccgccgcatc cgctggtgat accttccgct gcgagctgtt
    12181 cgatccaatc agcagcaaaa gagccgacag gaacatcaga gaagataccc gtggccaccg
    12241 gcgggacata agccgagcca tatttgccct tcaacaaaaa gatcgccatc tgggctcggg
    12301 tcacattgga atcggggcag taattaccgc cgccgcaccc gctggtgatg ccttcggaag
    12361 caaattgttc gatccacgcc gccgcgaaag tactggtgga tacatcgccg aaaaccgtac
    12421 ctgtagccgc cgggggaacg taggcactgc cgtgttcacc gcgcaaaatg aaaacagcca
    12481 tctgcgcacg ggtcacactg gattcggggc agtacatcat tggcgaagaa ctacaaccgc
    12541 cagtgatgcc agcgttgtat aaacgctcaa taaagttata agcccaatac gtggagggga
    12601 catccgtaaa ggtacctgaa gaaacctcaa gcggaggatt attctcagag aaatactcgt
    12661 ggctatcggc gttcattacc gcctgggcag gattgctgat cgccagagcc ttggccgcag
    12721 tttgaccgta ggcatagtca tcggtgccag ccaccactgt gaagtgggtg atttcatgaa
    12781 tgagtgtgcc cgccttcgaa tccgtgccgg tggtgggggc gctccaaaat gctccgcaca
    12841 aatagatctc ataaggagaa tccgggtaca cgtaggcata cacaccagca tcagtacaag
    12901 tgcagtcaaa atgcatgggg ctggccgtat ccaccgcgtt ttgaacattg gtgaaatggg
    12961 atttcacgag gttgtaacgg gcggaatcaa gcacaccaaa ccaggtgaca tagcgctcac
    13021 cctgtttatt ggccgcaaaa taagccacat catcggcggc atacgtgctg gcggcattgc
    13081 gggcgttcac cagatctgtc tgctgggagg aggaacagcc gttaaacgta tttgtgccca
    13141 ccacagcttg cggatccaca cgctgaatga cgcgattcgc gcggccttca acgaacaggt
    13201 caactgaagc agaaaccaac aggtcggtgc tttgtgactg gtcccagcgg tataaattcg
    13261 ccgaggcaat gttgtattgc accgaataat tgcccgaagc agaaaggtcg taatattcag
    13321 caaggtctac ttcgctggaa atacttcccc cgggcggcag ggtaatgtaa tcggcatcgg
    13381 tgggagccat gcgtttggca agtttgccaa ggtaggcaac agcttttcca tcacgggtga
    13441 tggagaaaat cggcgcttca actcccatgg caggcgtaag ccatttgagg attttgatct
    13501 gggaatttgt cgggttggag atgttcactt tgacgaggac cctgtcaccg gtcccgaaag
    13561 aagcgcggtc cacacttaat ctgacagtgg ctccacccaa tgagccttgg gcgctggcgg
    13621 cggatacatc ccatgaaccg aaagcggcga gggccaacat cagaatcatg gccacataaa
    13681 acaacttgcg tgcatttcct atcttcatta ttcctccgat gggtaaattt gcagggcgca
    13741 ttaactgaat tagtgagcgg gtgctgcgcg ccctgtgatg aacattttaa gcggagttga
    13801 actcagtcgt ccattggtca actcaccgat ttctttttcg atgtaaagtt gcagcgatgt
    13861 gacatcgtac atgacctcat aatcgtcggt ggcagacagg gcgtaatatt ctgccaggtt
    13921 cacatctgca gtgatgctgc cgcccgcctc cagtgtgatg taatcggctt cggtgggctc
    13981 ggcacgtttg agcattctac cgatgtaggg aacggtttct ccgttacggg tgatggtgaa
    14041 taatgaacgc tccagtccat ttatgggggt aaaccatttg agaatacgga tcggggtgtc
    14101 gttggggttg gtgatggtga catgcacaag gacatcgtcg ctggctgcaa acgaaccttg
    14161 gtctatgctc agggtgacgg ttgcgctcat cggtgctttt gcagaggctc cttgtgtatt
    14221 gaaatttctg aagacaaaga cggcaattag aatcacaaga accgccccgc cgaccaggat
    14281 catgcccttc aggttttggc gttttgtatc catttcttcc tcttttgcgc gtgtaatgtg
    14341 gacaatacaa atccagtata agtcaaacta cataaaaatc aagtaacaag tggggatgac
    14401 cctttcatac ccctgcaact cgtttgccaa attaccattt tgtgctttag aatcagccca
    14461 agatgcgaaa aatattcagc ggaatcggcg cattcctgca aaccccggtc ggtataatac
    14521 tgacaacaaa tgtaatctgg catattgtgg tgttggtggc gaaaatccct tttaattact
    14581 tcgccagcgc cgtaatcctt gggcttgata ttttcgccgc gttctatctg cgcgacaaac
    14641 tcatttactt cttttcccag ttcgtcctcc ccattcaaaa cccaaagcac cgccgtgaaa
    14701 ttgccgctcg tgtgagcaat tttgaatctg gcaggcgcgg ccccgccctt tttgtgaaaa
    14761 acggacgggt gatcctgcat gaaggtgaag caggcaagcg cggtgcaggt gtgatcgtgc
    14821 ttgataccgc cagcgccctc gtattgcgga cggataccga aatcaaggat actgttggcc
    14881 cgggcatcaa gttcaccagt gggaacgagt acattgcagg cagtgttgat ttgcgttcac
    14941 agtggcagtt catcggtcca ctggcgagcg accagccttt tctaaaccct gcttccaacc
    15001 catccaaggc cagtaacgag cgccgccagc aaaccagcgg catgacccgc gatgggttcg
    15061 aggtctcccc cacgatcagc atcaagttca gtatcaagcg tcccgttgaa aagaaaccga
    15121 ccgaaagcgg agtcacctca caatatgggt tcgatgccga agcagtgcgc aatgccatca
    15181 cccgtgaggt catccagctt ggcacaaccg aaaacaccaa ggtgcgcatg gaatggaaca
    15241 aactgcctgc gcatctggtt gtgaacatct ggcgcgaata catccgcaaa ttcaaactga
    15301 gtgacctgtt cacctccagc ggggtcagcg gattgcaaac catcgaagac atgatcaaca
    15361 agcgcgtcaa acaaagcaat gtgatggcgc tggatgatac gggctcgccc acaggtgaat
    15421 ggctcgaaag cctcgaattc aaacaactcg aatcacgcgg acttgaaatt caggaagtta
    15481 gtattcacaa cgtactcttc gaacaggaga tcgagaatca ggtcatctca caatggagca
    15541 ccgagtggat gaacctcgcc aaaaaagagg aaggccaact caaggaaaaa gaggcgctca
    15601 ttgaaaccgc ctcccgcgat gaaggcagca agagttttgc acgcattgcc tcacgccaat
    15661 tcagcggcaa acccaccatg ccacagcaaa acccattcaa gacactgcaa ctgctcatcc
    15721 agccgttgaa agagttcatc ctttttgaaa gcagtgccaa caccgatgtt gaaaaagagt
    15781 tgaggaagct cgatgacatt tggaaatggc ttttggataa caaccccgat ggtccacgaa
    15841 accagcaggg aggccagcca tgaacaaggc ccgccgtgat gaactcaaca ccctgctctt
    15901 cagccttacc gatgaaggcg tggattttag aaaccgctgg cgcagcatcg cgcagatcgt
    15961 cgtttggctg acgcttagcg gcatttccat gctgtcggct gttttcttca cacaggccac
    16021 ctggcagacc gcagttgtca ttggcatgag tctggtgaag tatgcgccgc tcatggcggt
    16081 tgtgtacagc ctcgcccgga aaatggcatc cctgtacctt gatgatattt tcgaattaca
    16141 cgatgaagaa ctcgcctccc aattcctcga agaagtgacc ttcggttacg gcctcgataa
    16201 aatcaccatc aacgaaggcc gcgtggtcga aaaagacgaa acctccccca tcatcctcat
    16261 tggcgggcca ggtgcgatcc aagtgaacct cgatagtgtg gccctgctcg aaaaagtgaa
    16321 cggtgaaccc gaagtgatct atccgcgcaa agatgcgtgg aaactcggca ggttcgagcg
    16381 catccgcgag atcggcaagt ttgacgaagt aggcaaacgc gaatacgcca tcatcaacct
    16441 gcgtgaccaa ttcatcagcg gccttacggt caaatcgcgc accaaagacg gcatccccat
    16501 cgaagcgcag gatatcaagg tcatcttcag catccttcgc aaaccaaaac ccgaaaaccc
    16561 cgaagcgccc gatagctctt atctcttcga tgaagaggcg gtcaaggcgc ttgtctacaa
    16621 ccaaaccatc atcacgcctg tgccatccac tccctcggcg gtcagttttc cgtgggatac
    16681 ctctgccatc ccgctcatca cctccgaact tgaaaacctg atcacctctc acaccttgag
    16741 cgagatcctt gtcaccatcg gccagaagga aattgacaac acctccaaca atgagcagac
    16801 catcacccaa atgcgggtgg agttgacagg ccagcagacc accctcggca gcaaaaaagc
    16861 cgcctcggca cccagcttca aatcacgttc acgcatcacc gcccaattct tcgaaaagcc
    16921 atttcaagat aaggccgccg cggtcggcat ctacattgaa tggatcgaca tcggcacatg
    16981 gcagctccca tcctcactca ttctcgacaa gcacaaggaa gcctggaacc ttgcgcgtga
    17041 aaacgcaaaa aaggccgcca gcgtagagcg tctgcgtaaa cgttttgaaa ccgctgaaat
    17101 cgtcagcctg atcgaaaatg tggtcatccg caactacgaa agacccgtca gcggcagcta
    17161 ccgttcctcc tactctggct ccaaagaaaa agaagccgac aaggaatggg atgccttcat
    17221 cgccgccaac ccagacctca aaggccaatt gaatccgcgg gaaagccgac ccaaagattc
    17281 caccgccacc gccaccgcgc tggaaattct caaagccttc cgcaaagaac tgctggcagg
    17341 caaggaactc attgaaaaag aaaacagacc tttcgaagaa aaacaagccg accttctcaa
    17401 gatcgaaaag gcgctcttcg atatctcaca tctcacccac cattggataa gaaaatcata
    17461 gtgaaaaaga acaacctcac cctctcgctg gaagtctcac gcgcactcaa tcatggcgcg
    17521 ccgctcgtcg ccctcgaatc caccgtcatt acacacggcc tgccccaccc gcaaaacctg
    17581 gaactggcgc gggatatgga aaagcagatc cgcgaggcag gtgcgactcc cgccaccgta
    17641 gccctcctgg atggagagat tcgcatcggt ctctctgacc tggaactggt tcggctttct
    17701 gaagcagact caaccctcaa agtaagccac cgcgatttcg ccaccgccat cgtcaaaaaa
    17761 gcggacggcg gcaccaccgt ggcaggcacc atgttcgtgg caaacatggc gggcatcaaa
    17821 gtctttgcca cgggcggcat cggcggcgtt cacaaagaat cgcccttcga tatctccact
    17881 gacctgcgct cactggcaga gattcccacc atcgtcgtct gtgctggcgc aaaagcgatc
    17941 ctcgatctgc ccgccacact cgaatatctt gaaaccatgg gcgtgcctgt catcggatac
    18001 cgcaccgacg aattccccgc tttctactcc cgcgaaagcg gactcggagt cagcgcccgc
    18061 ctggacaccc ccaaagagat cgccgacttc gccaaagccc actggaattt gggcatgaag
    18121 agcggaatcc tcgtgacgaa tccaattccc gaagcagaag caatttcaaa gtctgaaatg
    18181 gaaccgatga tcgctcaggc gtctgctgaa gccatcgcgc agggaatcca cggacagaaa
    18241 ctcactccat tcctgctggg ccgcatcagc gaattgacca agggcaaatc gctcaaagcc
    18301 aatctcgccc tgttgctcaa caacgcccgc ctcgccgcag agatcgcaaa agaaatgagc
    18361 ggaaaaaaga gagaatgggc gatataggaa atgatgaagg ctgaatttac gctcacagcg
    18421 acattcaatg ccagcgccga taaaatctat caggcgtttc tctccacgca gggacatacc
    18481 ctgatgaccg gcagccccgc caaagtggat ggcaaagtca acggtgagtt caccgcctgg
    18541 gacggttata tttggggcac gtttctggaa ctggaagaaa acaaaaagat cgttcaggca
    18601 tggcacaccg ctgaattccc cgaagacgca gctgattcac acgttgaaat tttattggaa
    18661 gaagcaaacg gaaaaacaaa actgaccttg attcacaccc acattcccaa aggtcaggca
    18721 gaggggtacc agcaaggctg ggaggatttt tacttcaagc cgatgaggga attctttggg
    18781 ggatagagag tgaatggtga atggtaattg gtaattaatg tctcgcgtgg acggtggtct
    18841 ggaaagacta ctggaacacg cgagactttt ttatttcaaa acaccacttt ctactttcca
    18901 cttctttctt ctttcctctt tcatccccca tcctttccct ccaataactc cagcaccaac
    18961 ttctccggta cctgcacagc caccacttcc ccacgcacag tgaccacgcc attgaccacg
    19021 atccattctt caacgaccac cttgcggcct ttgacttctt tcacatttgc gcgcacttcc
    19081 agcactggcc ccatcggtgt gggtttgaga tagtccacgt gcaatgaagc cgtgagatat
    19141 cgcaaaggcg gttcggtatc cattgcgcgg ttttcagcac gatagccagc cgcggctgct
    19201 gtccctgtgc cgtgacaatc gatcagtgaa gccagcagcc cgccatacac ataccccgga
    19261 atcgccgtgt gatacggctt cggctgaaaa taacaaaccg actcttcgct atcccaaaaa
    19321 ctcttgatct ggtgaccaag ttcattcaca ctgccacagc cataacaatg ggcaaaatac
    19381 tcggggtaat aatcttgaaa tgctttatcg gtcatgtaat tcacctttga aagaatttaa
    19441 ccacgaaggg tcacaaaggg tcacaaagaa gcgcatggtt ttcctttgta acccttcatt
    19501 cctttgtggg gatttaatta tggagtcggg gtcatcgtcg gcgtgctggt tggagtaggg
    19561 gttgccagtt cgctatgcac aataaactga cccaccactt tccaatcgta gcccatgaaa
    19621 atctgcactt catatgtccc cgcctgccac ccgccgatgg ggtcatcgca ctctgtataa
    19681 cccccgatgc cgcccgtacc gccattccac ggcgtggttt cataacagac caattgccca
    19741 tcgcggtacc acagtgcagt ccactgcaca ccggggaggg tgttgttgta atcgaagccg
    19801 ccatacattt ttgggatcgg aagttcaaaa accgttttcg ggtctacagg gttcaagccg
    19861 tcgaactcgg ttgaaaattg aagcggcgta aataatgcat ctgggtttgg agtcacaggt
    19921 ccgctgaaca gggcttcaat ggccacgggc aaaaatgggg taaaggtcag cgtgggcgta
    19981 tcagaaactg caggcgtggg cgtttctgaa ggaatcggtg taatggttgg cgtcagcgtg
    20041 atggttggcg tcagtgtaat ggtgggggtc aacgaagccg tgggcgatgg cggaaaaaag
    20101 tcgtacacga tcggttcgcc aaagaacgga agccaaaacg aacacccgaa caaaaccagt
    20161 gcaaataaaa acagccgcag cgcatttgca ttgtgcatcc gccgcaggct gtaaaaagac
    20221 atcttccgcg cattctgcat cacgcgcacc gaagcacgca caacgaacaa ggccgcaacc
    20281 gcaaccagta ttgccgcagc aatgattcca ccatgaatat ccataaatga aattatagca
    20341 ttttgtgcct tcgtgtcttc accactttgt ggttaaatgg ggaaatgaaa accgaaatcg
    20401 ttctcctcaa acttggcgga tcactgatca ccgacaagga catcccctac accccccgac
    20461 tggataaact aaaagaactg gccctggaaa taaaaacggt tctggattca acccccaacc
    20521 tgctcctgat tctggggcat ggatctggct cttttggcca cgtcgccgcg aagaaacatg
    20581 gcacccgcaa cggagtccaa accgcggaac aatggcaggg cttcgccgaa gtccgctttc
    20641 aggcggcgga gttgaatcgc tatgtgatgg aagcgcttta caacgcaggc gtcccggcga
    20701 tctcgttccc accatcttca tcactggtat ccaagaaccg aaaagttata gcgcacaact
    20761 ctttgactat acgcaaatct ttcgactttc acatactgcc tgtagtgtac ggtgatgttg
    20821 catttgatga ggcactcggc ggcacgatcc tctccactga agatgtgttc accttcctcg
    20881 ttgaacaatt ctcccccacc cgtattctgc ttgcaggaat tgaagcaggg gtatgggaag
    20941 atttcccagc gcggaccagg cttgtgaaac agattcagtt gtcagattat gaaaagatgc
    21001 gggctggcat tggcggttcc gtcagcaccg atgtcaccgg cgggatgaag gccaaagtcg
    21061 aagaaatgct cgacctcatc caaaagaatc cgaatttgac tgtacagatc ttctctgctg
    21121 aggagaatgg attgctcact cgggctttgc gcggtgaaaa tgtagggaca ttattgaagt
    21181 gaatccattt tagtgacaaa tttgggatga aattctcttg cgcttttcca aaaaaacttg
    21241 gataatagac aagctggatt atatataatt taaggagatt ctatggctac caagaagtct
    21301 gcaaaagcaa aggccgcacc caaggcgcgg aagattgtga agaaggcagt ggtaaagaaa
    21361 gccgccccca aggcaaaagc cgcattcact cccaccaaat taaaattggt gcgccctgtg
    21421 ccatcggata ttgatatcgc gcaagccgca aaattgaaac cgatcttgca ggttgccgaa
    21481 gaacttggcg tacgcccgaa tgaattggaa ctgtttgggc cgtacaaggc aaagatcaag
    21541 ctcgaaattt tggaacgcct taagaataaa ccaaacggca aatatattga cgtaaccgcc
    21601 atcactccca ccccgcttgg cgaaggcaag accacaacca cggtcggtct ttcacaggcg
    21661 ctcggcgccc acctcggcaa gaaggtgatc accgccattc gccagccttc gatgggcccg
    21721 acctttggca ttaagggcgg cgcggcaggc ggcggttatt cgcagatcat cccgatggaa
    21781 gatttcaacc tgcatctgac gggtgacatc cacgccatta ccgcttcgca caacctcgtg
    21841 gcagccgcgc tcgatgtacg cgtgatgcac gaaaaagaac aggatgatga aaaactgttc
    21901 aatgcgcttt gcccgcccaa caagaagggt gaacgcaaat tctcccccac catgctgcgc
    21961 cgtctggcga agcttggcat caagaagacc aatcccaacg atttgacccc cgaagaacgt
    22021 tcgcgcttcg cccgcctcga tattgatgag aagaccatca cctggcgccg cgtagtggat
    22081 atcaacgacc gtatgctgcg tgaaattgaa gtaggccgcg gcaaggatga agctggccat
    22141 gcgcacatgt ctggctatga tatcaccgtg gcatctgaga tcatggcagt gctggcgttg
    22201 accactggcc tcgaagatat gcgcgaccgc ttcggcagaa tggttgtagc aaccaacaaa
    22261 cgcggcgaag ctgtgaccgt ggaagacctc ggtgtggctg gcgctgtgac cgtattgatg
    22321 aaagacgcca tcaaacccaa cctgatgcaa accctcgaag gcactcccgc cactgttcat
    22381 gctggcccgt ttgccaatat cgcccacgga caatcctcca tcattgcaga tatgatcgca
    22441 ctcaagctgg tcggcaagga tggcttcgtg gtcaccgaat ctggtttcgg cgctgatatc
    22501 ggcatggaaa aattcttcaa catcaaatgc cgctactcag gtttgattcc gcaggtggtg
    22561 gtgatggtag ccaccgtccg cgcgttgaag atgcatggcg gcggcccgaa ggttgtggct
    22621 ggcaaaccgc ttgccccaga atacaccgat gaaaacctcg aactgctcaa agcaggtctg
    22681 cctaaccttg aacgtcacat tcagaacgcg cttaagttcg gtgtgaatgt ggtcgtggcg
    22741 gtcaattcgt ttgccaccga taccaaagcc gaagtggaaa tgatccgcga agctgcgttg
    22801 aagatgggcg cgatggatgc cgtcgtctcc actcactggg ccgatggcgg cgctggcgcg
    22861 aaggcattgg ccgaagccgt tgtaaaggct gcctccaaac ccagcaactt ccaattcctg
    22921 tatccgctgg aaaaccccat caagagcaag atcgaagaca ttgcctccaa gatctaccgc
    22981 gccgatggtg tggattacac ccccgaagcc gaagaacaga tcgaacgcta cacccgcctc
    23041 ggcttcgata aactgccgat ctgcatggca aagacgcatc tctccttcag caccgatgcc
    23101 accaagaaag gcgcccccac aggcttccgc atcaccgtac gtgagatccg cgcttcggtt
    23161 ggcgctggct tcctttatcc catccttggc gatatgcgca ccatgcctgg cctgcccacc
    23221 cgcccctcct tctacgatgt agaccttgac ctcgaaacag gcaaggtgct tggtctgttc
    23281 tagtcagatt tactttaata ccaacggccc ccgaagagtt ctcttcgagg gccgttttct
    23341 ttatatttta tgaaaaactt tgaaacttat tagttggggc actcacccat gttgaccgta
    23401 atggtgatcg aaccagcatc accagcagaa aggggcgcgc agcaattatt cgcggcatca
    23461 tagcccaacc cttcctgaca ttggcctgtg ccagtttgca aaccagcagc gctacaagcc
    23521 gtgttggaga atttcacctg gctggaccac gcctcagtgc catgacattc cactacatgc
    23581 ttgccatcca ccatggtgcc attatcggaa cagatcaagt tcgcaggatc aagcacttcg
    23641 aaagtcacac catcgtcaac aagaatattc tgatacggaa tcttctcaac gcagtaacga
    23701 tctgatgcaa ctgtatccgt aacggcaggg gcggcgcatg aagcagcagc ctgacaggcc
    23761 ggagcaaatg attgaacggt cgcaagtggt tgttcttgta caggtgcaga ttcaacaacg
    23821 ggcgcctgtg tcggcgcact ttctacaaca gggggcacag cggcctgggt ggccggggca
    23881 gccgagccac cgcaggcgga aacccacagg gcaagcagga cggtcattgc cagcaaaata
    23941 gtttttgtct ttttcatttt ttactccttc tgagtcattg atattaagac cttatcattg
    24001 taagatatca gtacagatag gctatgggag tattgtcctg tttggttata attgacctta
    24061 catgaacctt tcattcctgt ccctcactga tcagcgtgac ctcataaaaa gcagggaaat
    24121 caacgcctcc gaactcgccg atgcctgcta tcgtcaaatc gagcggctca acccgacgct
    24181 aaatgcgttc atcacggtta ttccgcccga gcaactctcc gcgcaggaag aaacgcaaag
    24241 cggccttctg caagagattc ccattgccgt gaaagacctg tacaacacaa agggaatccg
    24301 cactacagca gggacacgtt tttttgccga tcacattccg caggaagatg cctacgttgt
    24361 gcaaaaaata aaaaaggcag gcgcgcagat cattggaaag accaacaccc atgagatcgc
    24421 gctcggggtc accaacaaca atccgcatta tggtgcatgc agaaacccat gggatatcac
    24481 acgtaccccc ggtggttcct ctggcggcag tgcggtagcc gtttccacag gcatggccat
    24541 ggctgcactt ggaaccgaca ccggcggctc aatccgcatc cctgcctcat tgtgcggagt
    24601 ggtcggactc aaacccacct ttgggcgggt cagcttgcgc ggtattctgc cgctttcatg
    24661 gaatcttgat catgcaggcc cgatcacacg caaagtagaa gatgccgctc tcatgctgca
    24721 agtgatgggc ggatacgatg agcttgaccc cgcctccatg aagaccctgc caggcgatta
    24781 ttacagccac ctgcgcgatg gaatcaaaga ccgcaaattc gcactggcaa caggcagttt
    24841 tattgaagag tcagaccccg aagttctgga agcagttcgc aaaaccgcac aagtattgga
    24901 agaacaaggc gcggtcatca ccgaaaccaa tgtcgacttc ctcaaagaag ccgccgttgc
    24961 caacgccctg atgacccaag ccgatggtgc cgcttttcac cgcgaacgtt tgaaggaaca
    25021 tcccgattgg tttggcgccg atgtacgcca aagattggaa atcggcgctg cattcacctc
    25081 cagcgaatat attctggcaa ggcgcacaca ggctgaagtc aaacgccagc ttgaattgct
    25141 tttcgaagaa tatgatggcc tcctgctccc caccactccc atcacggccc ccgtactcga
    25201 aggcgaaaac gcactcgaac gtgcccgtca actcacccgt tttacagccc ccttcaacct
    25261 gaccggcctg cccgcactca ccatcccatg cgggtttaca agtgaaggtc tgcccatcgg
    25321 attgcagatc gtctcacggg catggaatga agcgggcgtc ctccgcacag gctacgcatt
    25381 tcaacaagca acagaatggc acacacgaat gccatccatc tagatcgcaa aaaatcctgc
    25441 cagactggca ggatttttat ttatctgccg cccgatttac aagtgactta taattaatcc
    25501 atgctgaaag ctacagactt ggttctggtc gctctcattc ccactccacg ggatatggag
    25561 atcgcccgct tgttgggatg gtatcgcatt ccgcttcgca ccgcgccgaa agttgttgcc
    25621 gtggactatc tggcctttta tcaacccgca gcattcggcg agcgcagcgg acaaatcgaa
    25681 tgcatcgccc aggtgaaggg ccacgaactg accacacgcg gtgaactcct aaaagatgaa
    25741 cgcaaccatc cgcgcgcgaa agaggaatac tacaaaattc aactgggcgg actcgaaaaa
    25801 ttaaaagaac ccatcaaaac cgacaaatgg aaacgcctca catttttgta ttccacaggc
    25861 gattacatct taaacgcaaa aactttgaac gacctggtgg tggaaggcgg agaacgtcaa
    25921 ctgctctgga aaaacctgcg cgaacgtgcc gagaatgagc agttatacaa aaccgatctt
    25981 cccgaggcag atattccccc ggaggtgctg atggcgctgc tggggatcaa ggaattgcaa
    26041 gagccatatg aagcaggcgc accaaatgcg caaaaagaaa atgaataaaa tgaaaaagac
    26101 agataagata gggaaaggat tcccaaagtt gattgtctgg caaaaagcca tcaactggtt
    26161 ttgatggttt acaaattggc tgaagctctc ccaaagcagg agctttttgg ctcttctggc
    26221 acatttgaca actctgaaca caattggagt aaaacatgaa caccctcatc aaaaacggca
    26281 ccctcgtcac cgcctctgat acattcgcag cagacattct gatcgaaggc gagaccatcg
    26341 cccagatcgg cagggatttg caacacccga atgctgaagt gatcgacgct acggacaggc
    26401 tcatcatgcc cggtggaatc gacccccatg tacatctcga cctgcccatg tttggcaccg
    26461 tctcatccga tgaccattac accggtcaca aggcggctgc gtttggcggc accaccaccg
    26521 taatggattt tgtcgtcctg gaaaatgaag gctttgacca ttccgtggac ctgtggatga
    26581 agaaagcgga aaaagccgcc attgattatt ccttccacat gaatctgacg aatttcaacg
    26641 acaagatcgc caaagagatt ccatcgctga tgaagatggg cattcaaacg ctcaaggtct
    26701 tcaccgccta caatggccgc ctgcgcattg atgacggcag tatcttcaaa gccctgcgca
    26761 ttgccaaaga aaacggcatg ttgatgatgg cccattgcga aaacggagat gtcatcgaag
    26821 cgctcatccc agaagcgctc aatgctgggc acaccacccc tgagtggcat gcatacaccc
    26881 gccctgcctg gggagccgtg gaagcgacct tgcgcatggc cgccatggca gagcaagccg
    26941 attcccctgt ttacatcgtc cacatgaatg cgggaggtga agtggatatg ctcaaatacg
    27001 cccgtgaaaa cggtgtcaag gtaatgggcg aaacctgtcc gcaatattta ttcttcacca
    27061 ttgaccatct gtaccgcccc gatggtgcca aatggatctg ctctcctcca atgcgtaccg
    27121 aacaggataa tgcccgttta tgggaaggtc tttcaggcgg ggttttgcaa acggtcggca
    27181 cggaccattg ccccttttat tttgacggta ccagtcccat cgtctatgaa ggaagcgaga
    27241 tcgccatccc cggaaaagaa ctgggcaaag acgatttcac aaagatcccc aacggcctgc
    27301 caggcattca agaccgcatg cccatcatgt ggacttatgg tgtgcgtgca ggctatatca
    27361 cgcccaatca atttgtggca tacaactgca caaaccctgc caagatcttc ggcctgtatc
    27421 cgcgcaaggg agcgctcatc ccaggtgcag atgcagatat tgtgatctgg gatcctgaga
    27481 aacaggtcaa atatggagtg gcacattcac atcaacgcac tgactataat ttgtatgaag
    27541 gctgggaact tgcagggtac cctgaaaaag ttttcctgcg cggaaagttg atcgtggatg
    27601 gcgaagaatg gaaaggcaaa cgcggcgacg gtcagttctt gaaacgcggc gcaggcgaaa
    27661 ttttataaaa gatacccctc aacaactaac tttacaggta aatcaaatca tgccagctaa
    27721 aaaaccgatc attcattgcg atgcgcttgt tgtccactgc atggactatc gcctgcaaaa
    27781 attcatccaa ccgtggatca cggtccgttt tggatacgat aattttgaca tcatttcgct
    27841 ggcaggcagc gtacatgatt acgaaatggt cttgaaatac gtacagcttg cggtactcat
    27901 ccacagtatt gaaacggtct gtctgatcaa tcacgaagat tgccgtgctt acgggcgcga
    27961 tggcacctac aaacgccaca aacatgacct gaaagatgcg caggccaaga ttaaaaaact
    28021 cttcccccat ttgcatgttg aaaccttcta cctgcatctg gatgggacat tcgaaccgat
    28081 ttcataaaag gaaacctcca tgacccacaa ttgccaggcc atcgtcgttc attgcatgga
    28141 ctaccgtttg caatcccata tcaatcaatg gctcgaatcc aattttgaaa aaggcactta
    28201 tgaccgcgcc gtaatggcag gcggtgtgta tgatgtgtac gatgtcatcc gtcaggtgga
    28261 tatcgccgcg cgcctgcatg gaatctcaaa ggtcattctc attaaccatg aagattgcgg
    28321 catgtacggc cccggcggca ccgaggaacg tcacgaagaa gaccttgata aagcagaaga
    28381 taaaattcgg cggctcttcc cccaccttga agtggatacg ttttatttaa atcttgatgg
    28441 caccttcgaa aaaaactcgt aacttatttt tttgcaagcg tttcgatggt cttcaaaaaa
    28501 gtcacggtca tccttgcggt ggcaccccac agcaactcgc catcataagg atgatatgcg
    28561 atcagtgacc gttctgaatc gcgcacgaaa aactcccagt aattatttcg gtctgaaagc
    28621 cacaacaggg gaattgtaaa cacccgcgcc acttcaactc ccgccacttt aaacgtgtac
    28681 ggccacggga tgacccccac gatcggactc acccgaaagc tgctgatggt gatcaagttg
    28741 ctgagtctcc cgatgactct gacatcggca ggccgcagcc cgatttcctc ctcggcttcg
    28801 cgcagggcgg tttgttcggg cgtggtctct ccctcgtcac aggcgccgcc ggggaaggaa
    28861 acctgtccct tgtgtgattg cacaatttca gtgcggcgcg tgaacaacaa atgccattcc
    28921 ccgttgactt gcgtcagcgg caccagcact gccgcacact tcaagcgtgt gtccgggtga
    28981 accgccattt ccgcgtatcc atcggcatct gtggaagatc gcatggccgc ctgaagtttt
    29041 tgcgtgatgt attcttcggt cagattcatg gctgggttta ccgtaccggg ctgccgcaat
    29101 acgcacactc ggcagtgacc tcgtcaagcc attccacttc atcggggcgc acggctgcac
    29161 cacaagatgg acaatgcgtg ggcaggacag gccgcttcgt cggggcggga atttcctttt
    29221 cacccgaagc atctggcagt gagtttgaac cgagcagggc cgatatttcc tccgcttccg
    29281 ctttcaatcc gcgggcctgc aattcatcaa tggcgcgttc acccaccata cgcatccgat
    29341 gcacccgccc ctgtgatgcg aagagggtca agccacggcg aaggtgggca acacccgttt
    29401 tggtctgccc gcccaaaatg gcggcccgcc ccgcttcaat gaaaagaaac ggagcgcgat
    29461 gtggaaaacg ttcctcggcg cgctggccaa gatctgcgaa aaggcttgaa gcctcttcat
    29521 attcgccatc cagcatgagt tgattggcct gctgcaaaac aggcggccct tcacgcctgc
    29581 cgccgcgaaa cattcgttgt tgacctcttc gacgaaacat catgaacctc cgttcttttc
    29641 aaacaataaa ttcacactga acaggatcga aacggctatt cttccatgcc aatctcttgt
    29701 tcgagttcct tcccgcgcgg atcggtattg ggttcgatcc ttctcccgaa gatcaaaaac
    29761 ccagtatggg caaccatgcg gtcggtcggg cggatgcggg ctgaatccgc tttgtagtga
    29821 cgcagcatta actcacagac ttccacaaat gcaaattgat ttttgcgcaa tgcgatcagg
    29881 gtcttttcaa cctgattgaa ggtgggaatt aaagtacaga agaagccgcc aggcttaagc
    29941 gccgcacgaa cctgtggaat gtaatcgtat gggttttgca catccagaaa aaaagcatcc
    30001 actccggtct cgtcgaagcc ttcctgaata ttgcgtaatt taaattccac acgggaggca
    30061 aggccaaccc gttccaggtt tttcttcgcc agggtttgtg tatcctgctt ttgttcatag
    30121 gaaatgacac ggccattcgc gccaacagcc gtggctagcg cgatggtcat cgagcccgag
    30181 ccagtaccag cctccatcac ccgctgaccg ggcgcaacac ccatttgaat caggatgtag
    30241 ccgatatctt ttggatacag aatttgggtg gcacgcggca ggtcattgag aatatctgca
    30301 atggatggct gcaacagaaa aaacggcgcg ccagtatggc taaatacctg cgaaccccag
    30361 ggcttgccga tcaagtcatc atgtttgatc atgccgcggt gcgtatgaag ttcagcacct
    30421 gccttgagcg aaaaaataaa atgtttgtgt gtcagcccca ccaattggat caggtcatta
    30481 tcgtgtgtaa ttgtcgaatc aatcatgcag ttattttacc ttttcttccg tctctgccct
    30541 ggtttcggca ggaggggtcg ggctaatgac ggctacactg cttaactttc tgttcgctgc
    30601 cagcagcaca acaaacatcc ccaccccaag cactaccaac ccgcccagca tggacggggc
    30661 atacgcggcc agccattccg gcctgccaag cgactcgcct actgaagaaa tgcccatgcc
    30721 gatcgcacag gaattccaca acccatgaat ggcaacggca caaaaatacg cggctggcaa
    30781 ccgtaaatag cgccgctctt gaaacgccga aacgatgccc caccccacca aacccgaggc
    30841 aaccatgtgc agtaaactcg tcccagcacg cacactcaca accacaaccc attcggctga
    30901 gccattcccg cttgcattga ggctttccac cagggcaaat gcaccaccgc tcaacatccc
    30961 cattgcaaat ccctgggctg gcgaagtgaa tttccgcgca aagagccaca ctcccaatgg
    31021 tttcaacagt tcctcgatca gcggcacaat aaaaccgatg tacccgatca gggttgcgat
    31081 caccgccggg ctggaaaggt agggagccac cagattcaga atcacatcct ggtctgtttc
    31141 attctgcaga attctgccaa cagccgcaac ctcttgcagc agatcgggct ggctgagtgc
    31201 cagaaatacg gtgcccgcga tgatgatcac aaataaaatg ataagttcca acacgatcat
    31261 ggtgaccggt gcaagggtca tgccaaggcc caaaatactg aaaaccctcc agcgtgaacc
    31321 aaagtcaatt ccctttgtac ccaacccaaa gaaaatccag atcggcggca cgatcacaag
    31381 caaggtcaag aacggaagca ccacccaggc caatgcaatc acttcagtat aaataactgc
    31441 cgcacccagc atcaccgaaa ggcctatcaa acccagcacg gccaagatct gccaattggc
    31501 aaatgggaat ttgaattgga gatcggcttg ttcgcggctc tgcgctttct ggaacacgaa
    31561 ccacatgcac accagcaaga ccagcaattc aaacccaaat gcaaatgtgc tgatcatttc
    31621 cccggcaggg tccccgcttt ctgaaaacag gtcaatgatc gagctgattc ccgtagcggc
    31681 gatgaacaaa aacacaaaca gcacacttgt accaagtacg atcagaatta atatcgaagg
    31741 ccaatgggta ttttttgtct gcattatttt tcttcaatct ccacactgat gatcttgtcg
    31801 ccgggcgcag ggatgaccgc ctgatcgggg tcacgtggtg tcaattgctc ggccacgtcc
    31861 aatccgctga taacctgacc aaaaatagtg taaccgccat caaggtgaga agcggggcca
    31921 aacgtaataa agaactggct tccgttcata ttcaaccctg aattggccat gcccaccaca
    31981 ccgggtttat caaatttcag atcgctgaat tcattattga aaaaatagcc ggggttgccc
    32041 ctgcctgtac cgcttgggtc acctgcttgt gccacgaaac caggcaatac ccgatgaaag
    32101 gtaacgccgc tgaaccatcc ctgccgggcc aggaacacaa acgaattcac cgccagcggg
    32161 gctttatccg gaaacaactg tatcacaatg tcacccttct cggtcttcac cgtggcaatg
    32221 tattgcttca cgggatcaat atcaaaaggc ggacactcgc taaactgctt ctgcccaagc
    32281 gcgatcagcc caaccgtctc gcttaagttt cgataattga ggatgttggc tgactggggc
    32341 gaattattga tgaggaccag cggcacagcg gggatcctca acttcttcgc cgcctcatac
    32401 atggacctca tgctggcttc tgtttgagga gcattcatcg ccgctgtgag tttttctcca
    32461 tccaacccca aaatgacagc ttcccgcacc acccaatcat cgaacgcgcg cggctccatg
    32521 tttatccatt cctgttgacg caaatagagc aggtcgtaca tattccaaaa ttgaccctgt
    32581 tcccccgccg caatcgcagc catggtggcc ttgtctgatt tatccatcac atccaataat
    32641 ggcactggcc gaaacacaaa gcggacattt tcctgactgc gtaaaaattc tgcgaatacc
    32701 agcgccatcg cgccacagcc cggtgattga aaatcgcaat actcaatgat ggtcactggg
    32761 gcatcggcag ggccgtagga aaaatcatcc gcggtggctg gcgggaagta cgaatccaca
    32821 cctggggttg gggtcggcgc aacaacgatc gcagagcaac ttgattcgga aatgggcgtg
    32881 gccacatcgg gcaggctggt cggctgaacc gccacagtaa ccggggcagt ctcggcttca
    32941 gcggcacacg cacttagacc gatcatactt acaatggaga ttatcaaaaa aaatacgatt
    33001 cgtttcatgg tttcctgatt ttgatttcag actttcccgc ttttgccgta cagaccgctg
    33061 cattggcaaa atctgcaaat gggaattgac tctctcccgc catgttactt tttgattgct
    33121 tcctgcaatt gctcgaaatt cgtcatccac gaaatggtgt tgacaaaatc ggccaaagat
    33181 tcgctttgct cacgcagggc acgcacagca gggccaacgg ggtcttcgcc agccgcgcca
    33241 ccgggtgaat cggcataggc accgtaaaat gcaaaataag cctgattgat cttacgaagc
    33301 aagtatccat tttgcagaaa aaggaggcgg cgcgcttcca tataggcttc cgcttcttcg
    33361 atctgacctt cggcaagcat ggcatccact gtgatgcgcg tttcgtgcat ttccgcgcgg
    33421 aaatcaaagg gggggcgcaa aaagtcaccg ggttcggggt ggtcaaatgg cgcagagatc
    33481 aggcccgggc ttaagcgcga agcgggtttt aattcagggt aaaagcgttc gatgacaagc
    33541 gctccgatct cgttcccggc gatggaagcg gttgtctcat tcatggtgcg caactcgggt
    33601 gtgttggcat acaaaaaacc aagcggacgc agggtcaggt agttgtgaat ccattcgtgt
    33661 gaaatggtat tgagcaccca cggcaggttc gtactgcgca tgaccatggt tgggtatacc
    33721 cccaccccgc cgatcggcac caccaacgat gaggtgttga ggcctgcatc cacacgactt
    33781 tcaagggcgg cctgctcatc aagagtgatt tcagtattga tggaaatatt tgcgatctgt
    33841 tcaatgcggt cacgcgggga aacaatgagc gcatatggca gcggtgtgga gtgataccaa
    33901 acattcggca caggctgccc aacagcagca aggccaattt ccgagaggat gatggttacc
    33961 tgttcctgca gggtggcttc agccaatggg gcaagtttgg cctgccgcgc gtattgttca
    34021 tcaagttcgg cgcgcaattc ttccgaggcg agttccttgt cagtaatagc ggcatcggca
    34081 tagatctgct gaagctgata ttcgttttcg aggatggatt gtgtgacacg caaatattca
    34141 gtgacgatct gcttgcgtga ttcgtagttg agtgtgtacg gcaccccaac cgatgcggag
    34201 cgcaatttga gcagggcagc attgcccatc catgccatgt aattgaattc gatctggcgt
    34261 gtaaatgcgc gtaccttttc gatcttatct gtaagggatg ggttgccgtg tccggtaatt
    34321 gcggcaatga tgaacaagat cagggtgatc tcaagcccgc gtatgatgcg ttgaaacatg
    34381 ggttggatta tacccgtacg ggcaggacgt aacaaaagcg cagggcggga cttgcgtccc
    34441 gccctgcttt ataccctgcc ctcacaacag gttatttttt atttttgagg tattcatcaa
    34501 tggccagtgc ggcttttctg ccgccaacca ttgcggtgac gacgaggtcc gggcctgtga
    34561 cacagtcgcc gccagagaat acgccggggc gtgatgtgtt gcccctggct ttgtctttca
    34621 cggtgatcaa accccaatca tgaacttcga gttcgggggt ggtctttcca atgatgggat
    34681 caggccagta gcccaacgca agaatggcag tatcggcagc cacgctgaaa ttggagcctt
    34741 ccaccgggac cggtttgcgg cgacctttgg catctggttc accgagtttc atttcgatgc
    34801 attccacagc ggcaagcttg ccatctgaac ctgcaatgaa ttttacaggc tgggtaagga
    34861 agcgatattt tgcgccttcc tcgcgggcca tcttgcggtc tttcttgcca ccgggcattt
    34921 ctttttcggt acggcgatag agacaggtta catcttttga gccgagtctg atggcggtgc
    34981 gcagacagtc ggaggcagtg tcaccgccac cgatgaccac aacacgcttg ccaacttcca
    35041 gcggcgcacg catgtgctca gggagttgat cgcgctccac attaccgcgg atcaggaaat
    35101 ctgttgcttc gtacacgccg ggcagttcgg tgcctggggt gtcttccatt tttgcgtcca
    35161 cttcagagcc gacgccaatg aaaacagctt caaagccttc ttcaaaaagg ccgtcaatgg
    35221 tcttatcttt accgatgtag gtattgggaa cgaatttcac accagcgcgt tcaaactctc
    35281 cccatttttc ctgccatact tccttggaga gtttgaaatt cggaatgcca tacacaagca
    35341 aaccacccgg cgcaggtttg gattcaaaga tggtcacatc gtgccctttt tgaacgagca
    35401 cttcggcaca gcccaaacca gcagggccag caccgatcac agccaccttt tttcctgagg
    35461 ggatcccgag cgggatgaca taaccctgtg tgcggcgttc ataatctaca gtaaaggctt
    35521 ccaattctcc gcacagcaca ggttggtgat gtttgtttaa gacgcatgaa ccctgacaca
    35581 aagcttcatg cggacataca cgtccgcaca cttcgggcag ggaactggtt ttatgataga
    35641 tccccgcagc ttccacaaaa cgtccctgtt cgatcaacca catggccgag ggaatgtcat
    35701 tggcggttgg gcaggctacc atgcagggag caggatcagg gcaatgaata caacgcgcgg
    35761 cttcaaccat cgcgcgttcg gggtcaaatt caatgactac atcatcaaaa tcacaagttc
    35821 gaacctctgc cgggcgaagg tcaaggtcga aaaaggggcg attggcacgc tcttttcgat
    35881 caatggccaa aaattcgctg ggaacagcct gcataatacc tccagaaaca aaaaaaaggt
    35941 ttccactaca tataaaattg cctgcaacct acacatgatg atactaaatc tacttgtaca
    36001 gacacaggct tatatgtcac caaacacgat gacaatcatc acaattttac cattagacat
    36061 ttcttaattc ccgtaccgaa aagagctcct tttcgtattc ctaaattaga atagaaagaa
    36121 accaaacctg cacaatttct acacattcat tcgttaaact tacaagataa atgaagtaag
    36181 aatacaagga agcacattga ctgtcttcct ggaggataaa tcacaatgaa acaatttttt
    36241 tcaaagatca cccgcagaac gtgggtcatc atcggcgttc tggcggtggt cgtgcttgca
    36301 atagtatttt ttagcacaag aagcagcgga gaacctcttt accaaaccac caccgttgaa
    36361 cgtggcaatc tggttgccac agtcggggca acgggtagtg ttcgtgcaag acaaagcgct
    36421 gtactcatct ggcagacgac tggcatcgtc aaagatattt atatggaagt tggaaacccg
    36481 gtcaaacgtg acgagatact ggccagcctc gataaaaatt ctctcaacca gagtgtcatc
    36541 ctcgcagagt cagatctggc tgcggccgaa aaggcactgg cagacctgct caactcagat
    36601 acagcccgtg cccaggcttt gcgcacagta gatgccgcgg aagaagccta taaaaaagcc
    36661 tatgattacc gactggaact gaacggtctt attaccatca ctgaaaccta tcttgtacgc
    36721 ggcgttccca aagtccgcac gtacaaaggt tatgctgatg ccgaaaccat cgccgacgct
    36781 gatgaaaagc tcgccctggc agaggctcaa ttaaacgacg cccgccgcaa tttggaccgt
    36841 ctgcaaaatg gtgtggaccc cgctgaacta gccgcagcag aaggacgcgt ggcagcagca
    36901 caatccacca tgaacatggc gcatatcatt gccccatttg atggggtcat cacccaatca
    36961 gaaccttctg ttggagatca ggtaactgct ggcacggtcg ccttccgcat tgacgatgtc
    37021 tcccacatgc tggttgatct gcaagtctct gaagtggata tcaacaacgt agccatcggc
    37081 caacccgccg tattgacctt tgatgccatt cttggcaagg aatatcacgg tgaagtggtt
    37141 gaagtgggtc aggctggtga cacggtgcag ggtgtggtca gttttacagt gacggtcgag
    37201 ttgaccgatg ccgatgaact tgtaaaacca ggcatgaccg ccgctgtgaa tgtggtgatc
    37261 gaagaacaaa aagacatgct gctcatcccc aaccgtgcag tgagattggt ggatggtgaa
    37321 cgggtggtgt atctgctcgt tgacgggcag cctgttcagg tcaagatcaa gctcggttct
    37381 tcatccgaca caatgagcgt tttagccgaa ggcgatatca aggaaggcga tctcattatt
    37441 ttgaatccac catccaataa tggcgggccg tttggaggct aatatggatc tggtgatcga
    37501 agctcgcgat cttcgaaaaa catatcagat gggcgagatc atcgtggaag ccttgcgcgg
    37561 cgtctcattc accatcgaac gcggcgaagt ggtttccatt atgggacctt ccggttcggg
    37621 caaatccacc atgatgaata cgctgggctg cctggatagg cccacctccg gcgattacat
    37681 tctggatggg gaatcggtcg ctgaaatgaa cgatgaccag cttgccagtg tgcgcaaccg
    37741 caaggtcgga ttcgtctttc agagtttcaa cctgctctca cggcttaccg cacttggaaa
    37801 tgtggaactg cccctgcgct atgccggcat caccgaaggc cgccgcgaac gagcacaggc
    37861 cgcccttgaa gcggtgggcc tgaaagaccg tatgctgcac cgcccctatg aactctccgg
    37921 cggtcagcaa caacgtgtgg ctgtggcgcg ttccctggtc aacaaccctg ccattattat
    37981 ggccgatgaa cccacaggca acctggattc aaaggttggc aaggaaatca tgagcctttt
    38041 gctcaatctc aacaaagacc gcggcaccac cctcatcatc gttacccatg accccaccat
    38101 cgcagagcaa acccagcgtg tcattcgtct gcgtgatggc gaattggacc ctgcatctga
    38161 aataaaaagg aagggatgag attatgagct tttcacaagc ccttctcgaa gccatcgaaa
    38221 gtttgaacgg aaataaaatg cgctccggcc tgaccgtgct cggcattgtc attggcgtgg
    38281 cagcggtgat cgccatgctt gcagttggca atggcgcaca ggcatccatc acaggttcca
    38341 tcagcagcat cggcacgaac ctgttgttcg tattcaaggg cagtcccgat ggtccgcctg
    38401 gacccggtgg cggcggtcgc ggaagcggaa ataatgaccg tcccctcacc ctttcagatg
    38461 cagaagccat tgcagatccg ctcgccgcac catcggtgga aattgtagct cccgccattc
    38521 aaggcaatgg aaccattacc tttgctggcg aaaataccac caccaccatc tcaggtgtca
    38581 caccaaacta tgcaccggtg cgcaaccttg agatcgccga aggtgagttc atcaacgaag
    38641 aacacatcct cggaagaatg tcggtggtag tccttgggcc agaaacagcc aatgccatct
    38701 ttggccacgc cgatggcatc gtcggtgaaa cgatccgcat cgaaggccag ccgttccgcg
    38761 tcattggtgt gctggttgcc aaaggcggcg gcgcatttgg cagtgaagat aacagcgcct
    38821 acattccatt caccaccgca caagcgcgcc ttatcaaacg cacttctctc gatgagattg
    38881 acgtactttt tgtacaagcc accaccgccg aatcagtacc acaggccgca gatgagattt
    38941 cgaacatcct gcgccagcgt caccgcaccc ccatcggcga tgacgatttc accgtattca
    39001 cccagcaaga ttttctacag accttcgaaa ccatcaccgg cgtactcacc atcttccttg
    39061 gcggtattgc aggcatctcc ctgcttgtgg gcggcatcgg catcatgaac atcatgctgg
    39121 tttccgttac agagcgcacc cgcgagatcg gcctgcgcaa agcactgggc gcgcgcaaaa
    39181 aagacatcct tctgcaattc ctgaccgaat cgtccctgct aagtttgatc ggcggcgtta
    39241 tcggagtcat cttcggctgg ttgatcgcgt tcgcagtggg gcaggtcgcc actgctacag
    39301 gtaacaactt cgtccccatc gtcggcaccg atgccatttt gctctccacc agtttttccg
    39361 ccatcatcgg cctgttcttc ggaatctacc ccgccagccg cgccgccaac ctcgaacctg
    39421 tggaagccct gcgctacgaa taggcaagtg aatgaccaaa acttttcaaa tcaaacgaag
    39481 catcctcacc gccgctctca tcgtggtctt gcttcaggca tgtatgcaag ttagagtgga
    39541 tgctcccctc gcactgccca ccccgcagga tattgaaacg gtggcggttg aatcgccagc
    39601 cgccacagcc accgcctcca ccaccccgac aactgcccct gccacagcga cagagactct
    39661 cccttctgca acaccccagc ccgaggtcgt cattcacgcg gtcaaaggga atctcttcat
    39721 ccgtcgcggc ccggatatgg ccttcaatcc aatcggtgtg ctttataaag atacaagcac
    39781 ccccgtgctt gcacgtgacg ttctctccaa ctgggtacag atcgtcatcc ccgactctga
    39841 ccaaaaaggc tgggtctccc tgcaaaccca atactctcaa gtggatggcg acctccgaac
    39901 cctgcccgaa ctcgccccca ccgattggcc tctcccagcc tatctgcgaa actgcaccca
    39961 ccatcaaatg tacatcctgc ctgccgaggt cattttgctc tcctcgcttg atcaccccga
    40021 taacatggtc tggctcaacc ccggcaccta caccgtctat gacatggatg tggctggtga
    40081 acccgaagtc accactgtgg aagtacgcga aggcgtcacc gtggaaattt tagacgacgg
    40141 tgcaggcgag caccgcaaat gcccataacc cgaactggca aaagccaccc tttcacgggt
    40201 ggcttttttt atccgcaaat ttcaatgaac cccttcgcag ggaggatgta aaatcacccc
    40261 gcatgggata aaatcaaaga aagcagtaat tgatcactga aattcatcaa tttgacgaaa
    40321 gtcgctcaat ttacccaatc tggaggtttt tgtgtctgaa aagttaaatc agttaaagga
    40381 aattctcgga gaagtcaacg acctatacca tgccgcatcg gtgcttgaat gggaccaaaa
    40441 tgtgagcatg ccccctggcg gcagcgaagc ccgtggacag caactctcga cattaggcaa
    40501 gatcgcccac gaaaaatcca cctccgacga agtcggcaag ctgcttgatg aactcaaaaa
    40561 ggaatacacc gaccccaaca ccgatgatgg cgccctcatc cgcgtggctt cacgcaacta
    40621 cgataaatcc acacgggtac ccgcctcctt cgttgccagg caggctctca ccacctccaa
    40681 agcctttgat gcctgggttg aagccaaagg caaatctgac ttcgccctct tccaaccgca
    40741 ccttgaacgc gtgctcgaac tggtgcgtga atatgtctcc ttcttcccgc ctgcagatca
    40801 cccctacgat accctgcttg atgactacga acccggcatg aaaaccgccg aagtacaggg
    40861 cattttcgaa aaactgcgcc cgcagcaggt gaaattgcta aaagccatca agtcttccaa
    40921 acaggtcaaa gacgatttcc tgcaaaagaa gtacaacgaa aagaaggttt gggccttcag
    40981 cgaagctatc atcaccaagt tcggctacga ctggaatcgc ggcaggcagg ataaagcccc
    41041 ccaccccttc caaaccacat tcagcgtcaa cgatgcccgc atcaccaacc gctacgaacc
    41101 caacaacccg atggccaccc tcttcagcgc catgcatgaa gcaggtcacg ctttttacga
    41161 actcggcagc aaccccgcct acgaacgcac accccttgca ggcggcactt cactggccgt
    41221 acacgaatcc caatcgcgcc tgtgggaaaa ccttgtcggg cgttcgctcc cattctggga
    41281 acatttctac ccagacctga aaaaagcctt cccctcccag ttggacggcg tcagcctcaa
    41341 agccttctac aaaggcatca acaaagtgga atcctctttc atccgcgtca atgccgatga
    41401 agccacctac aacctgcatg tcatgctccg cctcgaacta gaagtcggca tggtggatgg
    41461 aagtttcgca gtcaaagacc tgcccgaaat ctggaacaca aagatgcagg aatatcttgg
    41521 aattgttccg ccaaacaatg ccctgggcgt tctgcaagat gtccactggt ccaatggctt
    41581 gatgggatat ttctccacct acgcgcttgg caacctcgtc tctgcccagc tttgggaaaa
    41641 gatcaacaaa gacatcaaag acctcgacga tcagatccgc aagggcaaat tcgataccct
    41701 gctccactgg ctgcaagaaa agattcacac cttcggccac aaatacgacc cgcaggatat
    41761 cgttcaaaag gtcactggct ccaggatcga ttcagccgcc tacgtacgct acctgaatac
    41821 caagtacagc gatatttacg gcctgtaaaa aacacatccc gcaggcaatc tggagaacga
    41881 atcgaaactc catcactgcc tgcgggatat tgattcctgc gcccatgaaa aaacaccccc
    41941 tgcttctcgc ctctcttctc acgctgataa ctgcctgcac acaattaaca ggcccagccc
    42001 ccacacagac tcccgctcac tccctgccca ccgttgtcgc gttgacagga caggccgcct
    42061 tcgccacagc cagtgccttg gtccccacct ccgccccgcc gaccgaaacg ctcgttccaa
    42121 cagaaacacc tgtccccacc accccactcc cctccgccac accaactgtc gccgcggggt
    42181 ttaacgactt cgccgaaatt cacttcctgt cgccggggcc aatgtcgagc cttgtatctc
    42241 cgatcaatct gcaggtcatc ctcattgcag gcgaaagcga gatcgtacaa gtggacctac
    42301 tcggtgaaga cggccgtgtc cttgagcggg tgctggaacg cgtgcagaga aacctggcag
    42361 gcaactatcg cagttttgag ctttcctttg agatccgcgc cgtttctgaa aaaggatata
    42421 tccgcatcag ctccaaagat gaccacggac gaattcaagc gctcaacacc atgcccgtgc
    42481 ttttatattc cgtcggcgtc aatcaggtca acccgttagg caacatgatt tacgaacgca
    42541 tcatctatga aggtcttgaa gatggtgatg aaattttcgg cggcaccatc aacctcaaag
    42601 gacgtttctg gccgttcaat gagcaacctg ctttccttga gttaatgctc cccgatggtg
    42661 aggttatctc atcacgggtg ctcgatttca acggcatcga aacacaggaa tttgaaacca
    42721 ccctgcccta caaggtcact gaacccactc tggcacgcct cagcatccgc caggacaacc
    42781 ccgatatcag catcagcgac cccgatctca aaaagtatgt ttatatctac accatggaag
    42841 tcatgctata tccataagaa tgtgaccaag ttccgtcaga cattctgatg cac
//
LOCUS       DAOQJC010000017        42743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_383_length_42743_cov_8.933471, whole genome shotgun sequence.
ACCESSION   DAOQJC010000017 DAOQJC010000000
VERSION     DAOQJC010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 42743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42743
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_383_length_42743_cov_8.933471"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1613)
                     /locus_tag="PLE39_04050"
     CDS             complement(3..1613)
                     /locus_tag="PLE39_04050"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNE67526.1"
                     /translation="MTTKYLFFTGGVVSSVGKGVTAAAMGLLLKERGFNVAVQKLDPY
                     INVDPGTMSPYQHGEVYVLDDGAETDLDLGHYERFIDNRLTRVSNFTTGQVYAETIAK
                     ERRGDFLGGTIQVIPHITNEIKRRIGLAAKETGAEIMILEVGGTVGDIESQPFLEALR
                     QLRNEVGRENCLFVHVTWLPTIGATGEIKTKPTQHSVAALRSIGISPNIIIARADQDV
                     DKGLCEKIALFCDVEKDAVIPMKTADVLYEVPLLLEKLGVGELFLEKLGLKAKRKPNM
                     RPWKKLVEDVRKPKPTVKVALVGKYVELQDAYMSVREALKHAALANDVEVEINWVHSA
                     DLEKDKGWDIVKKSDAVLVPGGFGSRGIEGKVMAARFARENKVPYLGLCLGMQVMCID
                     FARHVLNLEDSNSSEFDRGTEHPIIDLMIDQRSITDMGGTMRLGLYPCLLQKGTKAAT
                     AYGVAKVDERHRHRFEFNNSYRADFEKHGMVFSGLSPDSRLVEIVEISDHPYMVASQF
                     HPEFLSRPMRPHPLFVGLLKAAKERAKK"
     gene            complement(1739..2113)
                     /locus_tag="PLE39_04055"
     CDS             complement(1739..2113)
                     /locus_tag="PLE39_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNE67527.1"
                     /translation="MAKSRSDQMVERLRNMQAAAPDIEASAVVSVDGLIMASALQQGV
                     EEDRVSAMSAAMLSLGERISSELGRGGLDQVYIKGDKGAIVLTAIGDEAVLTAMSRPD
                     GKLGMIFLEMRRAAEDLVKLVG"
     gene            2291..2917
                     /locus_tag="PLE39_04060"
     CDS             2291..2917
                     /locus_tag="PLE39_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67528.1"
                     /translation="MLNLPIAMLKLPVIFALFFWMQSTTAAITSPQPGDTLRGQVEIL
                     GTMDVPNFASAELAFSYDAPSPAESWFTIQTFPQPVQNPTLAVWDTTTVTDGDYTLRL
                     RVFLTDGSSQDVVVSGLKIRNDEPLTTATPTSTAQADIQLFETAAPPTLTPRPQPTLQ
                     TFPSPTPLPPNPASVTSSSIYVNFGRGALIVLVLFIFVGIIFRFRSRA"
     gene            2914..3306
                     /locus_tag="PLE39_04065"
     CDS             2914..3306
                     /locus_tag="PLE39_04065"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNE67529.1"
                     /translation="MTRPRACAAIVQNKKILMVLHRSSSREYWTLPGGGIVPGETPEQ
                     TAVREVKEETGLDTTMGNLLFFEPSGEYCFSAELANPASPILGYDPEESELPKVERLL
                     QAVAWLDLVEMKTDRQVSKVISALGIEL"
     gene            3303..3887
                     /gene="pth"
                     /locus_tag="PLE39_04070"
     CDS             3303..3887
                     /gene="pth"
                     /locus_tag="PLE39_04070"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNE67530.1"
                     /translation="MTDTTYLIIGLGNPGREYKDTRHNIGFMLVDQLAVRLDARGMKL
                     QSKAIVISAHHEERKIILAKPQTYMNLSGQSVQGLLHFYKIPIENLLIAHDDMDIPFG
                     TIRIRPGGGPGGQRGMASTIDHLGTKEFPRLRLGIGRPPGRMDPKDYVLQMFSKEDMK
                     LLPDVLSRGADAALEFVLKGLNSAMNKFNGEVGK"
     gene            3945..7337
                     /gene="mfd"
                     /locus_tag="PLE39_04075"
     CDS             3945..7337
                     /gene="mfd"
                     /locus_tag="PLE39_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNE67531.1"
                     /translation="MQPLLSNIRTLPAYQQLLVELNAGKTIAGLGLSRSARLPVLAAL
                     QADLNRPILLVTDRADHALSIFDELDFWVKSPRLLFAEPNPLFYEEAAWGVSTRRSRL
                     QTLTTLSTYHLPFSKKPETPPIIVASVRSLMTRTLPRRDYLKACKKLSVNQRIQPDGL
                     IREWARIGYQRVNTVLEPGQFSHRGGLLDIWPPTELNPVRLDFFGDEIETIRQFDPAT
                     QRTVEKLENILITPAREYLQPEGEPSENLSEFHIPLLHSQPASVLDYLPPQALVLMDD
                     ISLVETMANEIEEQAVKFRGECIAEGTLAAAFPVPYVPWSDLNDDIHHMPCLELGHST
                     ELLESESSVDLASNFSHDERFGGRLKPFIDYLASILERDEQVYIVSRQTPRLRELWAE
                     HYPNSEPENLSFLEASLSEGFTFQTSHLITDSEIFGWERPQPRIRQKQVAETPESIYA
                     DLQTGDYVVHVDHGIGRFNGLIQRQLDGHEREYLTVEYDRGDILYVPVHQADRLTRYV
                     GADGGAPSLDHLGGQAWQETKAKVKEAVQQVAEDLLDLYARRQVVEGYSFGADTQWQK
                     ELEDSFPYVETEDQKRAILDIKRDMERARPMDRLLCGDVGYGKTEVALRAAFKAVMSG
                     KQVAVLVPTTVLAQQHFETFSQRLAAFPVKVEMLSRFRTHREQTEILYKLALGEVDIV
                     IGTHRLISSDVEFKDLGLVVIDEEQRFGVTHKEHLKKLRTEVDVLTLTATPIPRTLYM
                     ALTGVRDISNLNTPPEERLPIVTHVGPYSPRLVRQSILRELERGGQVFFVHNRVNTID
                     AMKAHLNKLVPEARVDIGHGQMPEGQLAAVMHRFTMGEIDILLSTTIIESGLDIPNAN
                     TLIVDRADTFGLAQLYQLRGRVGRGAMRAYAYFFRHNKMSPTQEGQQRLEVIAENTQL
                     GAGYSIAMRDLEIRGAGELLGTRQHGYIQSVGFHLYTRMLADSVRRIRVLMKDGLNID
                     VPSALLPSAFSLPLSMPVNVDLPVAVGIPSDYISDQDLRLRLYRRIADLRDETELDAL
                     GSEFRDRFGHLPEMVQNLLYQMRVKLRAEKAGLSSVSWEAGQILLKYPAPAEGVEPKR
                     LPDLGEGVRGGKSAYWVTVAKEEAWTIRLLDVLDRLLKSPAS"
     gene            complement(7404..9446)
                     /locus_tag="PLE39_04080"
     CDS             complement(7404..9446)
                     /locus_tag="PLE39_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67532.1"
                     /translation="MKRIRSVLQNILSQEKYTPLAFLVVAFIGFGLLANRLGFYQDDW
                     PYIFYAFNKGIPSLTLELYYDSRPNAAWLYIVMFNLLGFKPIAWHLAAVIFRWLTGTL
                     LWYLLRRIWPSHNKQVTFATYLFIVHPFFLIQPYAVNSILYWAGFLFFSASLLIMARN
                     ATESKHRISLKLLAVFLEAVHLFTSEYFVGMVMVRPLILYWVLNSPELPTRQRIMTAI
                     KQWIPYLAALAAYIIWRMFLYVPPPIGDRNAPEVLFALFSKPLPTIAYLIRIALQDSV
                     IITFTSWYRTLVPELFAFSSIFSWFVILVSIFAFVAVFIHLKQITEKEERGSWFAQPL
                     TLGLFLLLLGMAPIWVIGQDIVTHKNQFAGTRFGIGSTLGAALILAVLIDRLIDGRKK
                     IVVVSACIALAISMHLTNEKDFAYSWEKQERFAQQLLWRAPKIEAGTALVTDEEILGY
                     MGSYSVSYSIITTYQPGDIQTPPHWYFPFYYTNPNVNDFLAGIPLEDSKLTMEFNGNS
                     DKMILFTFNPEMQRCLWVLRPEDTNLRLISTDMRQLSAGSDVELIQQGEKSPPEQIYG
                     KQNTDTWCYFFEKADLARQYKDWDSVVKLWKESQAIGEQADNGFEIIPFIEGYGHLED
                     WEQVKTLTKSAKRITAGLEPSLCGMLDRLALDTPASQERDDTLLKLKEDLNCSNYQ"
     gene            9587..11626
                     /gene="uvrB"
                     /locus_tag="PLE39_04085"
     CDS             9587..11626
                     /gene="uvrB"
                     /locus_tag="PLE39_04085"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNE67533.1"
                     /translation="MDFKLTAPFIPMGDQPEAIRGLVDGVRKGMKHQVLLGATGTGKT
                     FTIASVIQELQKPALIMAHNKTLAAQLYAEFKEFFPENAVSYFVSYYDYYQPEAYVPR
                     HDLFIEKETQINEEIERLRLAATTALVSRRDVIIVASVSCIYGLGNPEEFNKGSVNLA
                     VGQLVRRNALLRRLIESQYQRNDMELRTGTFRVRGDTLEIIPAYEEKRAYRITFFGDE
                     VERIMQFSPLTGEVLEEPREISIYPAKQYLTDAEKTKEAMADIESELEERLAQYKQAG
                     RILEAQRLEQRTRYDLEMLREVGYCSGIENYSRHFDRRAAGTHPWTMIDFLPSDYLLV
                     LDESHMTVPQIRGMYNGDRARKETLVEYGFRLPSAMDNRPLKFDEFEEVMGSTIYTSA
                     TPSAYEMDHAEQVVEQIIRPTGLIDPEVDVRPTRGQVDDLVGEIKQRVERRERILVTT
                     LTKRMAEDLTTYMKELGIKVQYLHSEVETLERVSILRDLRLGTFDVLVGINLLREGLD
                     LPEVSLVAVLDADKEGFLRSDTALIQTIGRAARNVNGRVIMYADRMTDSMKRAIDETN
                     RRRAKQMKYNEENGIVPISIHKEIHDLTEEMSAKAVGEMKGEYKVKGSQGMPRGELRK
                     IVDEVEKQMKEAAKNLEFERAAALRDELYELKTLLAEDESLKPWERIKLMTGEDD"
     gene            complement(11692..12120)
                     /locus_tag="PLE39_04090"
     CDS             complement(11692..12120)
                     /locus_tag="PLE39_04090"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HNE67534.1"
                     /translation="MYSPRHLEWTNALSTGDASLDSQHRYLVDTLNKLGDVINEGHGG
                     EELARILGVLRFYAGWHFGKEEDCMETYHCPAAEKNKHAHAVFVEKFDKYHAEFTKSG
                     GSNEMALKIHEEISDWIVNHILIVDGELYPCIHHKPKPTR"
     gene            complement(12246..12503)
                     /locus_tag="PLE39_04095"
     CDS             complement(12246..12503)
                     /locus_tag="PLE39_04095"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNE67535.1"
                     /translation="MDELTYEKIGEANSRAEAEVIESFLEAEGIDVELVEESISRTAY
                     TLATARVDIFIPKDEVKRALKLLQPFDDIQPDDEEEGNDQA"
     gene            complement(12537..13694)
                     /locus_tag="PLE39_04100"
     CDS             complement(12537..13694)
                     /locus_tag="PLE39_04100"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNE67536.1"
                     /translation="MQKNTPLLLAGAGPFGLSLAAYLKHEKIEHIHVGASMDYWKNFM
                     PLKMFLRSACDWHLDPHNEATIVNYLKTLNLQPADVEPLSLDFYLTYIEWFQRQKGIS
                     PLPAWITKIDQAGNGFDVTFADGSSTLAEKLVMAVGLRYFKNIPEPLNSLFPSGRFHH
                     TAEYIDFSNLKGKRVLIVGGRQSAFEWAALIHEEGAQNIHMTYRHDTPAFTPSDWTWV
                     NPLVESMADRPEWFRNLAQEEKDAIIKRMWGEGRLKLEPWLAKRITQETIHLHPQTQV
                     ADCKELPAGELQITLNDGSTFPIDHVILATGYKMDIAQIPMLANGTLAAKIKTQNGFP
                     VLDESFQSSVPNLYFTGLPAAQDFGPFFGFTAAANTSARIIGRALSMKQNS"
     gene            13844..14335
                     /locus_tag="PLE39_04105"
     CDS             13844..14335
                     /locus_tag="PLE39_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67537.1"
                     /translation="MKKTILIVGLLVLALGVLGVGVVFAQGTNPPYAGHGPMRNGEGP
                     LHTFMVIEFAKKLDLNVNDINTRIAAGETMYDIALSAGVTAEEFPTVMTEVRTAALDA
                     AVKANVITQEQADWMKSRGFGQGGMGTGACDGTGMQGAQGQGMGRGMMGQGRGWQNQQ
                     VAP"
     gene            complement(14403..15242)
                     /locus_tag="PLE39_04110"
     CDS             complement(14403..15242)
                     /locus_tag="PLE39_04110"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HNE67538.1"
                     /translation="MTQAAKAQSLLALHNNGKLLILPNVWNPIGARMLEVKGFPAVAT
                     ASAAIAESLGYNDGEQLKLESMLEMVRRIANSVEVPVTADFEAGYSETVAGLQQNISN
                     LLDTGAVGINFEDSWDDGARLRPIPEQVERIQAIRETAEKRGISLVINARTDSFFAEG
                     FASTEERIEDAVTRANAYLKAGANCIYPIGAGDEKTLKILRQRIHAPINVLASAKTAS
                     LKQLQEIGINRVSFGPYIFRSLLAKASAMYDEIKELGGYEVVTDGIFTGGDVQKYLLT
                     GKE"
     gene            complement(15252..16190)
                     /locus_tag="PLE39_04115"
     CDS             complement(15252..16190)
                     /locus_tag="PLE39_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNE67539.1"
                     /translation="MSHSHSHSHFGDLATQTTKRLSLSLSLTAAFVLVEVAAGIFGNS
                     LALLTDAAHNFTDVIALGLSWYALKIALKPANAGKTFGYHRVGILVALVNSTTLILIA
                     LGIFYEAWQRFLSPPEVDSAILMGVGALAFFINAVTAWLVQKGSEHDLNLRSAFLHLM
                     GDVISTLGAVIAGVVIYFTKWNWLDPFVSVLIGAFIVWNAWGILRQTIHILLESTPEN
                     IDMPTMISDIRKVHGVRDVHDLHVWSISEGLRMLSAHVVTDEISLRDGTFIQNNINEM
                     LAHEYHIQHATLQLECEGCQSSDLFCSIAEHHHAHH"
     gene            complement(16234..17637)
                     /locus_tag="PLE39_04120"
     CDS             complement(16234..17637)
                     /locus_tag="PLE39_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNE67540.1"
                     /translation="MATELKPNDILSKAESLRNNLSSGFRDEIVKSLYSEAEAIAKRA
                     VKTADNKRYDLDQKIDRLVTSPITGLPIMLILLSLIIWLTVSGANVASDAIANVLFAF
                     GEIGRNFFNSLGFIPWWITGFIWDGVYLGLAWVVSVMLPPMAIFFPAFTFLEDLGYLP
                     RVAFNMDWMFKKAGAHGKQSLTMAMGFGCNAAGVIATRVIDSPRERLIAILTNNFVPC
                     NGRFPTLIMLGTVFVAAGFSGLTSSVIAAGSVVGVVLLGVVFTFLMSWILSRTVLKGE
                     ASAFTLELPPYRKPNFGRILYTSIIDRTIFVLWRAMQTAAPAGAIIWILANVHIGDTS
                     LARAIADWLNPFGLLLGLDGVILLAYIIAIPANEIVVPTMMMMYMGAGMMIDGPSSNT
                     AIYDLLVHGNGWTMLTAVNLMLFALLHNPCATTIMTIYKETKSLKWATLSVVMTLGTA
                     FLVTFLTASIARLFGWA"
     gene            complement(17639..18475)
                     /locus_tag="PLE39_04125"
     CDS             complement(17639..18475)
                     /locus_tag="PLE39_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNE67541.1"
                     /translation="MTNTKTVLPTEHCETCPAFTQLEQMGIKVGDFDRVIALAGNPNT
                     GKSTLFNALTGLKQHTGNWPGKTVTRAEGGFEFNRMRYKLVDLPGTYSLLSASQDEEV
                     ARDFILFGQPNCTIVVTDATALERNLNLVMQVMEITENVVVAVNLMDEARRKGLEVDT
                     RSLSRDLGVPAIPITARTGEGIHTLLGVVAEVIEGKVEIKPLRVNGTPEFQKAVNELV
                     PMIEQVAPGIPNARWLAIRLLDGDARVQKALTSGELADIVSRQQRMDVQFSRKMSVEG
                     NQ"
     gene            complement(18504..19508)
                     /locus_tag="PLE39_04130"
     CDS             complement(18504..19508)
                     /locus_tag="PLE39_04130"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNE67542.1"
                     /translation="MNPIILLILGAALSALIFLPRVGLIAVYKTYREAKEREHVEDAL
                     KHLLDREQQGRHASPVSLAGTLDLPLAKTTQLIENMETQELLETRGNDLHLTSKGERW
                     ALHVVRAHRLWERFLADEARMPLEKVHDEAQRREHTLTDAQLNELDAALGHPTRDPHG
                     DPIPTREGTLPKAEGMPITAWQADRPARIVHLEDEPAIAYQQILAAGLRLGQVIRIIE
                     KTQGRYLLSDGETEYKLAPAVASNVHVTALPEIESLKSEAVPLAELAHDQRGEIVMLD
                     DAVQGFTRRRFLDLGLTPGTLIYPELKNFFGDPRAYRIRGTLIALRKDQAAQIWVKPL
                     "
     gene            complement(19505..20110)
                     /locus_tag="PLE39_04135"
     CDS             complement(19505..20110)
                     /locus_tag="PLE39_04135"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNE67543.1"
                     /translation="MNETIFRGIAAIVLISGMAISSYHRIKADRHTGEKVSRKTDGSV
                     MMGIIKLFGLLLWLSPFVYLIAPQWMAWSKIGLPEWARWLGVGLGVASDGLIYWLFSS
                     IGDGISPASATRKEHKLSTSGPYRWVRHPLYTVGTSLFISFGLMADNWFIALLGVLAF
                     IVMAIRTPKEEANLIAKFGDEYREYMKTTGRFLPKLGGKPS"
     gene            complement(20137..20805)
                     /locus_tag="PLE39_04140"
     CDS             complement(20137..20805)
                     /locus_tag="PLE39_04140"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNE67544.1"
                     /translation="MEQNLTISIQDYLKHIYELTENGTSASTNALAAKLKVKPASVTG
                     MVQKLASAKPALVTYQKHQGVTLTDDGKKAALEVIRHHRLLEAWLVQTLGYTWDEVHE
                     EAEKLEHVISEDFERRIAAAMGHPLRDPHGELIPTADLEMPFEDSTPLSSLRPECIVA
                     IQCVKAADTDLLRYLENLGLVPGAEIKIKAYSPYDHNLTIQIEKESIVLGLNITSKIY
                     VKEI"
     gene            complement(20807..21493)
                     /locus_tag="PLE39_04145"
     CDS             complement(20807..21493)
                     /locus_tag="PLE39_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67545.1"
                     /translation="MNSIQVTGLLAIVGATLYAIGDVLLLANKVNIDDYPKLKPFQKL
                     LSDAEKFVSIPSEKMMWGALIGVFSTPLVLAGFWQVYQGLADINPILKTATALLFVCS
                     SVIGAFVHGSFFYAGEYVKALNEVSEDSQNIFVELFTRHRKVLIVTYAPLLVMVVIAS
                     VLFSVAVATQSTMFPAWMAAINPVTMTIAWLLSKRILPAFITDALEGAGFNVAYFIFF
                     TCTTITLWNA"
     gene            21653..22804
                     /locus_tag="PLE39_04150"
     CDS             21653..22804
                     /locus_tag="PLE39_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNE67546.1"
                     /translation="MKKATHQQTKQHNRDLVLRTIFLQESISRADIARLTNLTRTTVS
                     DVVAGLIAEGLVCEVGHGASIGGKSPILLSVIADARYVVGLNLAQDKFIGALVNLKGE
                     IKETIELDVHESDGEKGLQFVYQIMDQLFKKKLKPIIGIGIGTPGLVNTREGVVLNAV
                     NLDWQDLPLGSLLEKKYKVPVSVLNDSQAAAIGEFVYGGDHRPDENLIVVNARYGIGA
                     GILINGRLFQGDGGGAGEIGHVVVQENGETCRCGKRGCLETVASVRAVLRQMNADSLE
                     AVSEAFLAGEARAVDIVTKAAHFLGVSLANLIGTLNIQKIVLTGDMTRFGPAWLSEVE
                     ASMRAAALTRMSGATKLELGKLDYRACILGAAAFLLLDDYSLLFNEENL"
     gene            22801..24177
                     /locus_tag="PLE39_04155"
     CDS             22801..24177
                     /locus_tag="PLE39_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNE67547.1"
                     /translation="MTFQLIPPRVVPPLDEGFRPAVLANRFFYQAATSPLLLGLERNG
                     GEVSRFETKILPPDHPDFEDNFLYVERIVKFLLWQRGGHTLHVGNSPRVAEYLQTLYS
                     AQGERKFDWEFMGEQVYERPLKILACALDEVPASRETGKLVGRNLQGCRIGFDLGASD
                     RKVSAVVDGTPIYSEEVIWEPRKNSDPEYHYREIVTAIRTAASKMPRVDAIGGSSAGI
                     YIDNRPMVASLFRSVPRERFGEIKEMFSKIQKEMNVPLEVINDGDVTALAGSMSIEDD
                     GILGIALGSSEAAGYVNMEGHIMGWLNELAFAPIDYSPSAPVEEWSGDMGCGSSYFSQ
                     QCVFRLAPKAGIDIPSEVTDAEKLKFVQEKLEAGHEGAVNIWRSMGIYLGYGIAHYAD
                     FYDMKHVLILGRCTSGRGGDLLIEGAKKVFEVEFPELLQKVELHLPDEKIRRVGQSVA
                     AASLPVIN"
     gene            24209..25060
                     /locus_tag="PLE39_04160"
     CDS             24209..25060
                     /locus_tag="PLE39_04160"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNE67548.1"
                     /translation="MKFHLDTAEIFVPDLEPVEKALARTTHLCLAAHQDDIEIMAAQP
                     IIECFQQKEKWFTGVVVTDGRGSPRNSLYENYTDDEMRLVRFKEQRKAAVVGEFAAQV
                     MLDIPSKVIKDVSRTEPVEDILKLLRATRPRYVYTHNLADKHDTHVGVALRVIAALRM
                     LDQSERPERIVGCEVWRALDWMVDSDKVLMDVSNHENLQAALLGVFDSQIVGGKRYDL
                     ASMGRRRANATYFESHGVDATTGLSYAMDMTPLMNDAKMDPAEFAQEFIQRFAVDVKS
                     RIGRMNA"
     gene            25057..26094
                     /locus_tag="PLE39_04165"
     CDS             25057..26094
                     /locus_tag="PLE39_04165"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNE67549.1"
                     /translation="MTLFSEINEQPERIKALLASQRKNVERIAAEIKKRDIEYVFLAA
                     RGTSDNAGRYANYLLGAMNGLPLALATPSLFTYYKRPPKLKNALVIGVSQSGKSPDIV
                     SVLEEGRKQGCQTLSITNEPDSPLAQASDFVLDIHAGAEKAVAATKTYTTELMSVAML
                     SAALSGNKAQWNDLSKVAAWMRQSLKQNDFLAQAAQRYRYIDQTVVLGRGFNYATAFE
                     WALKLKELTYIIAEPYSSADFAHGPIAMVESGYPVFAVAPKGKVFNSMLEMLNRLRSD
                     IAAELIVISNDKRALALAQVPLSIPVDVPEWLSPLVNILPAQLFAYHLTVAKGYNTEQ
                     PRSIRKVTETK"
     gene            26245..27216
                     /locus_tag="PLE39_04170"
     CDS             26245..27216
                     /locus_tag="PLE39_04170"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67550.1"
                     /translation="MRVCVLSDEIIDDFNPSPYLKDYDWEMVTLTAPVEEKIRALSES
                     KKFDVFLNVCEGYEFEDEEDHEQGYQAIEVVEALEKLGLPFTGADSNCFDPTREEMQA
                     IAEAHGVSFVKGYHVTGEDEARELVKNLRYPIMVKHPKSYGSTGMTRESRAETLEQVL
                     AQVKRICSEFGAARMEEFIVGKEYNVLVVENANDLSQPFAYPPAELVFPPNEDFWHVD
                     VKWNYDVPFDFNQVKDPALIEKLHDIAKRMYLAMGVSGYGRCDIRMNDQGELFILEIN
                     PNGGIMFKPEEYGPADYMILYDPDGYKGFFDRIFQAALARQKMRSKK"
     gene            complement(27305..28069)
                     /locus_tag="PLE39_04175"
     CDS             complement(27305..28069)
                     /locus_tag="PLE39_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67551.1"
                     /translation="MNKNTRIMVSSVAAIFVLACACPATSLPTINTQPTTPPFVVPPT
                     QAIPPTQDGFEVPTLPPLEDTNILMSDDFNEFSSRIETTSDETGSSEIKDGVYILRAN
                     GDVWNWGKSNSEFSDTVIEVDASMTVGPANNNAGIGIICRMQTREDTSIDGYLLAISA
                     DGYYSIRSILAGSMEPLVDWTYSDTIVQGSGHNIIRATCDGNSLILEVNGEQVATAAT
                     ISGGPESGPMAFSVVSFESAEPTVEVHFDNLVVTAP"
     gene            complement(28118..28528)
                     /locus_tag="PLE39_04180"
     CDS             complement(28118..28528)
                     /locus_tag="PLE39_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67552.1"
                     /translation="MENHEFDFWLGEWDVTWGEGVHGTNRVESILDGAVIQENFNGDG
                     LIGISVSVFSREDSRWHQTWVDNSGSYLDFVGEFVDGKMILSRNGIAEGKPIKQRMVW
                     FDISANQFRWNWERSDDDGANWRALWEIQYQRKA"
     gene            28671..29627
                     /locus_tag="PLE39_04185"
     CDS             28671..29627
                     /locus_tag="PLE39_04185"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67553.1"
                     /translation="MRVCILSDEAIEDYNPSVFFRAHEWDFFTVTPPVSDFIRQIVEQ
                     KNYDVFLNICEGADDNDADSGFSVVQTLESLGVPFTGADSKFYNPTREQMQTAAEARG
                     LNFARGFNARSVQDLSQAHALRFPLIVKHPNSFGSAGLTPHSRVDSFEELEEQFQRNF
                     SEYGSARVEEFIEGREVSCLVADNPDDLANPFAYLPVEVKFPVGETFMHMEVKWLNWD
                     TFIVPLEGEGLVRSVQEVSRQMFHVMGGTGYARVDIRVRPNGELVILEINPNCGILYY
                     GPDDRSMSDLPISWDKDGHDGFLDRIFRSAILRRDLRSGREK"
     gene            complement(29645..29809)
                     /locus_tag="PLE39_04190"
     CDS             complement(29645..29809)
                     /locus_tag="PLE39_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67554.1"
                     /translation="MINKRRASTIFLILGLVFLVIGIGMDNTTFSYAAIAFVLISLVL
                     GGRWLRPRKR"
     gene            complement(29806..31218)
                     /locus_tag="PLE39_04195"
     CDS             complement(29806..31218)
                     /locus_tag="PLE39_04195"
                     /inference="COORDINATES: protein motif:HMM:NF015368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67555.1"
                     /translation="MRPFEIVIPILLAIYLFWPNPRPLLIRLAPAFALAATLIHFTFE
                     GYRWQMIPLYVLSPILAASGLIKFTKVDSPSFSSVWKERIGRGLTLGLLAVSTVIPAL
                     LPVPQIPTPSGSLKIGTVIYEFTDTSRTELYSDDKSEPRRFMVQAWYPATPQKSDQRA
                     QWMSNAETFAPAISGYLNLPSFFLDHLALVNVPAYENAQAAKTDTGYPIIVFSHGWNG
                     FNAQNTGQALELASRGYVVIAVQHTYGAVVTVFPDGTVAPNNPQALPEDANDPNYEVV
                     ARKLVNQWAGDISFTLDQFAGQNQDSESIFFQTLDLSRVGVYGHSTGGGAAIQFCGTD
                     PRCKTILGMDPFMRPVSEEVLQNGLSQPSFFMFSQGWADVTDSKNNQLFNQFQPNLGD
                     NRGIIVISGTKHFDFSDLPLLSPIAPQLGLKGPLNGGRVVEIVNSYLLGFFEMTLKNQ
                     PSELFNGNFADFNEVKDRNQ"
     gene            31286..33319
                     /locus_tag="PLE39_04200"
     CDS             31286..33319
                     /locus_tag="PLE39_04200"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNE67556.1"
                     /translation="MILFSLSIFISAFLLFQIQPMIGKLILPWFGGTPAVWSTAMLFF
                     QVLLTGGYAYAYWLVKRPKQGWIHLAVLGGTIAILTTLGLVWSSPITPPANIHFAMTG
                     LPVLDVFLILAASVGLPYFVLAANGPLMQAWYSRIFPERSYARLYALSNIGSLLGLLA
                     YPLFVEPAFSVFYQGWGWAIVFFVFSVIVGILSWRTRNESEFGRNALRTETPALALHV
                     LWILLSGTASLFLLSVTNQVSQEVAVIPFLWILPLGVYLLSFVLAFSDSRWYERRIYA
                     LLFSLVSVAMLWVLTKTESLNVLVQIIVYNLLLFLACMICHGELYRLRPQADHLTSFY
                     LMVSLGGAAGGLFVNLAAPYLFSGYWEFYLGWLLVVVFLVALLWPGLHSRMERQAQIM
                     VAAFVLGAVLLALGLNREDGPLFVERNFYGVIRVFDWDGEANSMVHGVTVHGLQFLDD
                     RSRPTTYYVEDSGVGLLLLNHPRRGHGLRVGVLGLGAGTLAAYGETGDLYRFYEINPT
                     VVQLAEGAGGYFSFLSDSRAEVVTVTGDARISLEQELAKGQRQDFDVLVLDTFSSDSI
                     PVHLVTREAFALYLEHLAKDGVIAAHISNRHLDLRPILWLMAQEYDLSMIQIDRPASG
                     GDNGFPSSWVLLARDSSLLEIPAIKSHSADFDSYVTAIKPWTDDYSNLFQILK"
     gene            complement(33421..34182)
                     /locus_tag="PLE39_04205"
     CDS             complement(33421..34182)
                     /locus_tag="PLE39_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HNE67557.1"
                     /translation="MFFGFDLKEILMFPFKDQESRKYFLIGCLVALAAFFIPIVPYLV
                     LFGYAVRIARQVMNNESPRMIAWDDWGGMVKDGVKVFGIRLILVSPILILLIPLIISS
                     IAFPIVMNSVNSSEIESVATVFSLLVFASLCCIIPLSIPLGLIIPGAEMHVIEKDDFM
                     AGLRFKEWWPIFRANLSGFIAALAITYVVSMAVTLVIQILGATLILACLVPFLLLGMT
                     LHLTILMFVLGAQAYKVGKEKLAQQPEDSAPAPAE"
     gene            complement(34218..34856)
                     /locus_tag="PLE39_04210"
     CDS             complement(34218..34856)
                     /locus_tag="PLE39_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNE67558.1"
                     /translation="MLKKLREPVNSLTHWGGAILALAGLIALLIVGWSTPAKIISLTI
                     YGVSLIAMFSASATYHMVRVKDKALEIFRKIDHAAIFLLIAGTYTPFCINAFTGFWKW
                     GMMSIVWSLALIGIGVKIFYIRSPRWLNAGIYVLMGWISVIAGGQMLAALPTWVFVWL
                     LIGGVVYTLGAVVYATKIFNFIPGVFGFHEVWHIFVLLAAAAHFVAVMGVAL"
     gene            complement(34913..35425)
                     /locus_tag="PLE39_04215"
     CDS             complement(34913..35425)
                     /locus_tag="PLE39_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67559.1"
                     /translation="MENEQQQVLRKEALGNNVIRIIGILSFLFTLFSCAGAVMLDLVG
                     QKAIGKVSNAAVNCPPGKTCWTGRVDFTTEQGEQVTFHPLTAPMLFDFDPFLSGRSYA
                     DYGNYQVRYFAPFPKFAKVKLAYFLEYSTQVTGCCFGVFMLLIASAFSTKPGKPHKPL
                     VIDLSKFRNK"
     gene            complement(35478..36095)
                     /locus_tag="PLE39_04220"
     CDS             complement(35478..36095)
                     /locus_tag="PLE39_04220"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE67560.1"
                     /translation="MLMKNFMRVFFHLLYHPFAFTYDLVAAVVSFGQWKNWGRSILPF
                     IEGTRILELGHGPGHLQRFLLDLKLDPVAIDESAQMGKLARKRIGPTHKLTRGLAQTL
                     PFQTASFDCVISTFPTEYIFDPRTLSEIKRILKPAGRLVVVPAAFPSNRFLKWLYKVT
                     GENPQALDDNLKKRLVAPFTQIGFLTEVQIKEVKSSRVVILVAKK"
     gene            complement(36150..36506)
                     /locus_tag="PLE39_04225"
     CDS             complement(36150..36506)
                     /locus_tag="PLE39_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNE67561.1"
                     /translation="MSDRDEFGAFLVGFVVGGLTGAVVALLFAPQSGEETRALIKDKS
                     IELRDKAQMSAEEAYARAEQLAADARARAEELSKEARARAAELASEVRERGKGALEAV
                     RSKAGKKADGSEAAAG"
     gene            complement(36542..37003)
                     /locus_tag="PLE39_04230"
     CDS             complement(36542..37003)
                     /locus_tag="PLE39_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67562.1"
                     /translation="MATLPPTEQPTPPPLTPEQLAEIREQERKQKQMMYGIVAVIVLL
                     VALLGVAIYFLLQPNTPTDKIRDVFIIVVALETLVIGVALVVLIVQLASLINLLQNEV
                     RPILHATSETVNTLRGTAEFLGENVVEPVIKLNGYLAGLKRMLELMGIKPK"
     gene            complement(37060..37686)
                     /locus_tag="PLE39_04235"
     CDS             complement(37060..37686)
                     /locus_tag="PLE39_04235"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE67563.1"
                     /translation="MPNSFSIRPAVQADTLALSRLVIQLYAAELPGALTGPIDGQEAL
                     FRFTLEANGENALKNRYVMCDENGNILATGMIQFSDEPTFERAPDGTVSNAFRLLGTP
                     AALKLFLTVARSLIGVQKHEDMQSALIHSVVVDQNLRGKGIGVQLMTHLEERICEHGV
                     KKSRLQVLEDNISAVPFYQKLGYKQISKSPWWVSLLSWSSLGMEKNLA"
     gene            complement(37689..40868)
                     /locus_tag="PLE39_04240"
     CDS             complement(37689..40868)
                     /locus_tag="PLE39_04240"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HNE67564.1"
                     /translation="MISRIRAALNLREGEERTVFLMLAQYFFMGAAMLFVQSASFALF
                     FTAWDTTAMPYIYLGIAVIVSSITALFLKISARTSLAQFLILSILFLLFGTIALRIGL
                     AITTSKWLMLILPIWSQTLVNISVTAFWTLAGNLFDVRQGKRIFGLMNAGSWLAYVAM
                     GPFTTPLVNAIGTENLYTVIALCVLISFVLQQITLKKNPRTYATPESPEGETQKTSIL
                     HYLRNKYIFLIFALITTWRVSYFILDNIFYDRAALRYPSTAELAGFIGGFFGLVGLLG
                     FVTDMFLTGRIISRFGLRAGLLATPALTVLCTAALAVTGSINPNLILVLFWLAVAGKF
                     ANEGVGFSLDQTASALLYQPIAESDRIRAQTITEGIVQPLAIGLAGLLLLLFNTMLKF
                     DAIQLSFIYLLSGAIWIGICLALIRAYPNALTEALHKHRFGDKGIPLADLVSKEIVIN
                     ALKSPHPNEALYALELLEKSGSENFTEILIQQLRSPYPEVRIYAAKSIERLKINEALS
                     EIEVLFQKDAVPNVRQIAACTWSALSKNAKDALKLVNSPDTNTSHGVVVGLLRTRKIN
                     EVQEAYQHLRSLAGSEHAHEKLQAANLLVEIANPQMVEVLTPLLQDESIEVKKAALRA
                     AAKTHHPDVYPLVIAALASPQTRSLAFNALVAGRETALPEIIRSIKDESLHRRIHLRL
                     VRACAHIKGDAVHVLESWINHHNVSLRSRVLTALHECRYKPEGRSMQIVQNQIEVELR
                     RAAWLLSCLGDLQSTPKTEAIIRAFNDDLAETRDRLFYLFGFLHDPHIIHRARAAIAR
                     NDENRTAYAIEVMDATLSQSHKAAFIPLLENFPAAERLQKMGTRYKQDKPRTSRIIDI
                     LTNALAHENPWLVANAIEIAREVGIAESQVIIESLTGSKESLIVRIARQKRTEKNMLS
                     TVERVIILKSLRMFADTPDEALAELADLLQEMSVMPGETVIKEGETGESLYIIVDGKM
                     EVVDDNRVLNQLGARAVFGELSLLDSSPRSATVRALEETSLLRLDQTPFYEIMSDYVE
                     VAMGTIHMLTRNLRARTGDVLELSRILGQ"
     gene            complement(40980..41711)
                     /locus_tag="PLE39_04245"
     CDS             complement(40980..41711)
                     /locus_tag="PLE39_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE67565.1"
                     /translation="MNHKKFLQVFILLAVLVSSLGFTSGVSAYSCGTSYTVQSGETLS
                     SIAQTCGTTVAALQLANPKANPYIFVGQILVLPGAFWDNGNGYATYVVSRGDTLKSLA
                     ARFGTSMDTLASLNSIANYNLIYEGQRMTVPSGVAVPVPPPAAGRTYTVQQGDTLKKI
                     ATRINVTVNDILLVNPQITDANLIYVGQVISLPASASYYTVQSGDTMKKIAARFGTTL
                     DSLIVLNPQITDINKIYVGQVIRIW"
     gene            41848..42408
                     /locus_tag="PLE39_04250"
     CDS             41848..42408
                     /locus_tag="PLE39_04250"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE67566.1"
                     /translation="MTRDDVLDAAAQVFRQKGFHGASMSDIASALDVQKASLYHHVTS
                     KQEILLALLERALGMLTEHIASFAARTIPADQKLRLMIRGYLSALAENADLTSVLLFE
                     HRSLDKKSHTRHVPQRDRFEGLWHDVLDEGVREKVFDLKDTGLAVRAIMGTLNWTLTW
                     YRPDGPKSIEQIADEYSDFILKGLLK"
     gene            42449..>42743
                     /locus_tag="PLE39_04255"
     CDS             42449..>42743
                     /locus_tag="PLE39_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE67567.1"
                     /translation="MYSFEPNEEQQMLIDAVGKFASNDLRPAAHDAEESHEMSQKLIN
                     KGWELGLLQASTPEAYGGFGERSAVTTVLAVEEMAFGDLAGALAVMTPSLFATP"
BASE COUNT         9940 a        10428 c        11679 g        10696 t
ORIGIN      
        1 cactacttct tcgcccgttc cttcgccgcc ttcaacaacc ccacaaacag cggatgcggc
       61 cgcatggggc gcgacaaaaa ctcagggtgg aattgactcg ccaccatgta tgggtggtct
      121 gaaatttcaa cgatctcaac cagtctgctg tcgggtgaga gaccagagaa aaccatgcca
      181 tgtttttcaa aatcagcgcg gtaggaattg ttgaattcaa agcggtggcg atgacgctcg
      241 tccactttgg cgaccccgta agcggtggcg gccttcgtcc ctttttggag caggcacggg
      301 taaagtccga ggcgcatggt tccgcccatg tcggtgatcg agcgttggtc gatcatcagg
      361 tcaatgatgg ggtgttccgt tccgcggtcg aattcggatg agttggaatc ttcgaggttg
      421 agtacatggc gggcgaagtc aatgcacatc acctgcatcc ccaaacacag accgaggtac
      481 ggcaccttgt tttcgcgggc gaagcgtgcg gccatcacct tgccttcaat gccgcgcgaa
      541 ccgaaaccgc caggcaccag caccgcatcc gattttttga cgatgtccca gcccttgtct
      601 ttttcaaggt cggctgagtg gacccagttg atttccactt ccacgtcgtt ggcgagagcg
      661 gcatgtttaa gcgcctcacg cactgacatg taagcatcct gcaattcgac gtatttgccc
      721 acgagagcaa ccttcacggt cggttttggc ttgcgcacat cctcaaccag ttttttccac
      781 gggcgcatgt tgggcttgcg ttttgccttc agcccaagtt tttcaaggaa tagttcgccc
      841 acaccgagct tttcgagcag caggggaact tcatacaaaa cgtcggctgt tttcatgggg
      901 atgactgcat ctttttccac atcacagaaa agagcaatct tttcgcatag acctttatcc
      961 acgtcctggt ccgcacgggc aatgatgatg ttcggcgaaa tgccgatgga acgtaacgct
     1021 gcaacagagt gttgggtggg tttggtcttg atctcaccag tggcgccgat ggtggggagc
     1081 caggtcacat gcacgaacag gcagttctcg cggcccactt cgttgcggag ctggcgcagg
     1141 gcctcaagga acggctgcga ttcaatatca ccgaccgtgc cgcccacttc gaggatcatg
     1201 atctcggcgc ctgtctcttt tgcggcgagc ccgatgcggc gcttgatctc gttggtgatg
     1261 tgcggaatga cttgaatcgt tccgccgagg aaatcgccgc ggcgttcctt ggcgatggtt
     1321 tccgcataaa cctgccctgt ggtgaaattg ctgaccctgg tcaggcggtt gtcaatgaat
     1381 ctttcgtagt gacccagatc caggtcggtt tctgcgccgt catccagcac gtacacttcg
     1441 ccatgttggt atggcgacat cgtgccaggg tcaacgttga tatacgggtc cagcttttgt
     1501 acagcgacat tgaagccgcg ttccttgagc agcaaaccca ttgcggcagc ggtgacgccc
     1561 ttgcctacag atgaaacaac accgccggtg aaaaataaat atttagtggt catgattttt
     1621 ccttttctcg tccttctccc atggtgtggg ggtgaggtta acaaagagaa tggggagggc
     1681 tttaagccct ccccattctg gggttacgaa caatttgggt gatgaaattt ctttcggctc
     1741 atccgaccag ctttacgagg tcttccgcgg cgcgccgcat ttcgaggaag atcataccga
     1801 gttttccgtc aggacgggac atggcggtga gcacggcttc atcgccgatg gcggtgagca
     1861 caatggcgcc cttatcgcct ttgatgtaaa cctgatccag cccgccgcgg cccaattcgc
     1921 tcgaaattcg ctcgccaagg cttaacatag ccgccgacat ggctgaaaca cgatcttctt
     1981 ccacaccctg ctgcaacgca gatgccataa taaggccgtc gacggatacg acggcagatg
     2041 cttcgatatc gggggcggct gcctgcatat ttcgcaggcg ttccaccatc tggtcagaac
     2101 gtgattttgc cataactcag tctcctgggc cgttttgtga ttgattggca cgtagtgtac
     2161 cagaaaaata attattcgag aattgcttgc acatttcaat aatgtcattg caaggcagtg
     2221 ggtaaaaaat gccctgattt gggtcttcac ctcgcaaatt cacaacaaga tttgaccctg
     2281 cccctccttc atgttaaact tgccgattgc catgctgaaa ctgcccgtca ttttcgcgct
     2341 ctttttttgg atgcaatcca ccaccgcggc cataacttcg ccgcaacccg gggataccct
     2401 gcgcgggcag gtggaaattc ttggaacgat ggatgtgccg aactttgcct cggcggaact
     2461 ggcattttcc tacgacgccc ccagccccgc cgaaagctgg ttcaccatcc agacattccc
     2521 tcagcctgtg caaaacccca ccctcgccgt ttgggatacg accaccgtta ccgatggaga
     2581 ttacaccctg cgtttgcgcg tcttccttac ggatggttcc tcacaggatg tggtcgtttc
     2641 ggggttgaag atccgcaatg atgagccgct cacaactgca acgccgacct ccaccgctca
     2701 ggcagatatc cagcttttcg aaaccgcagc ccctcccacg ttgacgcccc gtccgcagcc
     2761 aactttgcaa accttccctt caccgacgcc tttgccgccc aaccccgcct cggtgacctc
     2821 ctcttcgatc tatgtcaact tcgggcgcgg cgcattgatc gtgctggttt tgttcatttt
     2881 cgtcgggatc attttcagat tcaggagcag ggcatgaccc gtccgcgtgc ttgtgcggcc
     2941 attgttcaaa acaaaaaaat tctgatggtc ctgcatcgtt cctcttcaag agaatattgg
     3001 accctgcccg gcggcggaat tgtgccaggc gagacgcccg aacaaaccgc tgtccgcgag
     3061 gtgaaagaag agacaggtct tgatacgacg atgggaaatt tacttttctt tgaaccatct
     3121 ggtgaatatt gcttttcggc ggaattggca aaccctgcat ctcccatatt gggatatgac
     3181 cccgaagaaa gtgaattgcc gaaggtggaa cgtcttttgc aggccgtggc ctggcttgat
     3241 ctcgttgaaa tgaaaacaga tagacaggta tcgaaagtga tctccgcact tggaattgaa
     3301 ttatgacaga caccacttat ctcatcatcg gtctcggtaa cccgggccgc gaatacaaag
     3361 acacaaggca caacatcggc ttcatgttgg tggaccagct tgcggtgcgg ttggatgcgc
     3421 gcggaatgaa attgcaatcc aaggccatcg tcatctctgc tcatcacgaa gagcggaaga
     3481 taatccttgc gaagccgcag acgtacatga acctttcagg ccaatcggtt cagggacttc
     3541 tccactttta caagatcccc atcgaaaacc tgttgatcgc tcacgatgac atggatattc
     3601 ccttcggcac gatccgcatt cgcccgggcg gcgggcctgg tggtcagcgc ggaatggcat
     3661 ccaccattga ccatctcggc accaaagaat ttccgcgttt gcgcctcggc attggccgcc
     3721 cccccggccg catggacccg aaagactacg ttctgcaaat gttctcaaag gaggatatga
     3781 aactgctgcc cgatgttctc agccgtggcg cagatgctgc gttggaattt gtgctgaagg
     3841 gcctgaattc agcgatgaat aaattcaacg gagaagtggg caagtaggga ttgaaaagta
     3901 aacatatccc agttcaattt ccacacttcg atctttctca aaccatgcaa ccccttcttt
     3961 ccaacatccg cacgcttcct gcttaccagc aattactggt tgagcttaac gcaggcaaaa
     4021 ccatcgctgg ccttgggctt tcgcgttctg cgcggttgcc tgtcttggct gccctgcaag
     4081 cggacttgaa ccgccccatt ttgcttgtta ccgaccgcgc cgatcatgcg ctttccattt
     4141 ttgacgaact cgatttctgg gttaaatcac cgcgtctgtt gtttgcagag ccgaatccgc
     4201 ttttttatga agaagccgcc tggggagttt ccacccgccg ttcacgcttg cagaccctga
     4261 ccacgctctc cacgtatcat ttgccatttt caaaaaaacc tgaaacgccg ccgatcatcg
     4321 ttgcttcggt tcgttcgctg atgacacgca ccctgccgcg ccgtgactat ctcaaagcgt
     4381 gtaaaaaact ttcggtgaac cagcgcattc aacccgatgg attgatccgc gaatgggcga
     4441 ggatcggtta tcagcgggtg aatactgttc ttgaaccagg tcaattttca catcgcggcg
     4501 gtctgcttga tatctggccg cccactgagt tgaatcctgt gcgcctcgat tttttcggtg
     4561 atgaaattga gaccatccgt caatttgacc ctgccacgca gcgcacggtc gaaaaacttg
     4621 aaaacatcct catcacccct gcgcgtgaat acctacagcc cgaaggggaa ccctccgaaa
     4681 acctgagcga atttcatatt ccgcttttac attcgcagcc tgcttcggtg ctcgattatc
     4741 tcccgccgca agcgctcgtg ttgatggacg acatcagcct ggttgagacc atggcaaatg
     4801 agatcgaaga acaggcggtg aaatttcgag gcgaatgtat cgccgaaggt acgcttgcgg
     4861 cggctttccc tgtgccgtat gtgccgtggt ccgatttgaa cgacgacatt caccacatgc
     4921 cctgtcttga gttgggtcat tccactgaat tactcgaaag cgaaagcagc gttgaccttg
     4981 cttcaaattt cagccacgat gaacgttttg gcggcaggct taaaccattc attgattacc
     5041 ttgccagcat acttgaacgc gacgagcagg tttacattgt ctcgcgccag acgccgcgct
     5101 tgcgtgaatt gtgggcggaa cattatccga actcagagcc tgaaaatctt tctttccttg
     5161 aggcttcgct ttctgaaggg ttcacgtttc aaacctcgca tctcatcacc gactctgaga
     5221 tcttcggctg ggaacggccg cagccgcgca ttcgccaaaa gcaggtagcc gagacgcccg
     5281 aatcgatcta tgctgacttg caaacgggtg actatgtcgt tcatgtagat cacggcattg
     5341 gacgattcaa cggtctgatt cagcgacaac tggacggcca cgaaagagaa tacctcaccg
     5401 ttgaatatga ccgcggtgac attctctacg tccccgtcca tcaagcagac cgactcacgc
     5461 gttacgtcgg cgcggatggc ggggcgccct cccttgacca tctcggtgga caggcctggc
     5521 aggaaaccaa agccaaagtc aaagaggcgg tgcagcaggt tgctgaagac ctcctcgatt
     5581 tgtatgcgcg ccgtcaggtt gtggaaggtt attctttcgg cgccgatacg cagtggcaaa
     5641 aagaacttga agacagtttc ccctatgtgg aaaccgaaga ccaaaaacgt gccatcctcg
     5701 atattaagcg cgacatggaa cgtgcccgtc ccatggatcg cctgttgtgc ggtgatgtgg
     5761 gctacggtaa aacggaagtt gctcttcgcg ccgccttcaa agctgtgatg agcggcaagc
     5821 aagtggctgt gctggtgccg accacggttc tggcgcagca gcatttcgaa accttttcgc
     5881 aaaggctggc ggcttttccc gtcaaagtgg aaatgctctc gcgtttccga acccaccgcg
     5941 agcagactga aattttgtac aaactcgccc tcggtgaggt ggatattgtc atcgggacgc
     6001 atcgcctgat ctcttctgat gtggagttca aagacctcgg tctggtcgtc attgatgaag
     6061 aacagcgttt tggcgtcaca cacaaggagc atctcaagaa actccgcacc gaagtggatg
     6121 tgctgacgct cacagccaca ccgattccgc gcactttata catggcgttg acgggtgtac
     6181 gcgatatttc caatttgaat acgccgcccg aagaacgcct gccgatcgtt acgcatgtcg
     6241 ggccatattc tccgcgtttg gtgcggcaat ccattctgcg cgaactggag cgcggtggac
     6301 aggttttctt cgtccacaac cgcgtgaaca ccattgacgc gatgaaggca cacctcaaca
     6361 aactcgtccc cgaagcgcgt gtggatatcg gtcacggtca aatgcccgaa ggtcaactgg
     6421 ctgccgtgat gcatcgcttc actatgggtg aaattgatat tttgctttcc acgaccatca
     6481 tcgaatcagg tcttgatatt cccaatgcca acaccctcat cgtagatcgg gccgatacct
     6541 ttggccttgc ccaactgtat caactgcgcg gacgggtcgg tcgcggcgcg atgcgggcct
     6601 atgcttactt cttccgccac aacaaaatgt cgccgacgca ggaaggccag caaaggcttg
     6661 aggtcattgc tgaaaatacc caactgggcg caggctactc cattgccatg cgcgatctgg
     6721 aaatccgcgg cgcaggcgaa ttgctcggta cacgccaaca tggatacatc cagtcggttg
     6781 gttttcatct ttacacacga atgctcgccg attcggtccg ccgaattcga gtgttgatga
     6841 aagatggatt gaatatcgat gtgccatctg cgcttttgcc ttctgccttc agcctgccgc
     6901 tttccatgcc agtaaatgtg gatctgcctg ttgccgttgg tattcccagc gattacatct
     6961 ccgatcagga tctgcgcctg cgtttgtatc gccgcatcgc cgacttgcgc gatgaaaccg
     7021 aactcgatgc gctcggctct gaattccgcg accgttttgg tcatttgcct gaaatggttc
     7081 aaaacctgct gtatcaaatg cgggtgaaac tccgcgctga aaaggcagga ctttcttcgg
     7141 tgagctggga agcggggcag atcctgctca agtaccccgc acccgctgaa ggcgtagagc
     7201 cgaagcgtct gcctgatttg ggcgaaggtg tccgtggcgg caaatccgct tactgggtga
     7261 cggtcgcaaa agaagaagcc tggaccatcc gattgcttga tgtgttggat cgtttgctga
     7321 aaagccccgc ctcttgattt cccgaggcct gattctgttt caataaaaat tcctgccccg
     7381 acaatttgtc ggggcaggct tgcttactgg tagttgctgc aattcagatc ctctttcaat
     7441 ttcagcagcg tgtcatcgcg ctcttgtgat gcgggcgtgt cgagcgccag ccgatccagc
     7501 atgccgcata gactgggctc cagccccgcc gtgatgcgct tcgcagactt ggtcaaggtt
     7561 ttgacctgct cccaatcttc aagatgacca tagccttcga taaacggaat gatctcgaag
     7621 ccgttatcgg cctgctcgcc aatggcttgt gattctttcc aaagtttcac aaccgagtcc
     7681 caatctttgt attgacgggc gaggtcggct ttttcgaaaa agtaacacca tgtgtcggtg
     7741 ttttgttttc cgtagatttg ttctggcggg cttttctctc cctgctgaat caattccacg
     7801 tccgagcctg cgctgagttg acgcatgtcc gtgctgatca atcgcaggtt ggtgtcttcg
     7861 ggccgcagca cccacaggca tctttgcatt tcagggttga aggtgaacag gatcatcttg
     7921 tcgctgtttc cgttgaattc catcgtcaac ttgctgtctt caagcggaat ccctgccaga
     7981 aaatcgttca cattggggtt ggtgtaataa aaagggaaat accaatgggg cggtgtttga
     8041 atatcgcctg gctggtaggt ggtgatgatc gaatacgaga ccgagtagct gcccatgtag
     8101 ccgaggattt cttcatcagt gaccagggcc gtgcctgctt cgatcttcgg tgcgcgccag
     8161 aggagctgct gtgcaaaacg ctcctgtttt tcccaggaat aggcaaaatc cttttcgttg
     8221 gtcagatgca tcgagatcgc cagcgcaatg catgccgaaa cgaccacgat cttttttctc
     8281 ccgtcgatca ggcggtcgat caacacagcc agaatcaatg ctgcgcccag tgtagagcca
     8341 attccgaaac gtgtccctgc gaattgattc ttgtgcgtca cgatatcctg cccgatgacc
     8401 cagatggggg ccatgccaag caggagcaga aacaggccca aggtcaacgg ctgcgcgaac
     8461 cagctgccac gctcctcttt ttcggtgatc tgtttcaggt ggatgaacac cgccacgaag
     8521 gcaaaaatcg aaacaaggat tacgaaccaa ctaaaaatgg aactgaacgc aaacaactct
     8581 ggcacgagag tccgatacca gcttgtaaat gtaatgatga ccgagtcttg caatgcgatt
     8641 cgaatcaggt aggcaatggt tggcaggggc ttgctaaaca acgcgaacaa aacttcaggc
     8701 gcgttgcggt ctccgatggg cggcggcaca tacaggaaca tccgccagat gatgtaagcc
     8761 gcaagcgcag cgagataagg aatccactgt ttgatggcgg tcatgatgcg ctggcgggtt
     8821 ggaagttcgg gcgaatttag aacccaatag aggatcagcg ggcgcaccat caccatgccg
     8881 acaaaatatt cactggtgaa gagatgaact gcttcgagaa aaaccgccag caactttaac
     8941 gaaatgcgat gcttggactc tgtggcattc cgtgccatga tgagcaaact ggccgagaaa
     9001 aataaaaacc ccgcccaata caaaatcgag ttgaccgcgt acggctggat caaaaagaaa
     9061 ggatggacaa tgaaaaggta ggtcgcaaag gtcacctgct tgttgtggct tggccaaatt
     9121 cggcgaagca aataccaaag cagtgtgccc gtcaaccagc gaaagatcac cgccgccaga
     9181 tgccaggcga tcggtttgaa tcccagcagg ttgaacatga cgatgtacag ccacgccgca
     9241 ttcgggcggc tgtcgtaata caattccagc gtcaggctcg gaatgccttt gttgaacgca
     9301 taaaagatat acggccaatc atcctgatag aagccgagtc gattcgcaag caaaccgaaa
     9361 ccgataaagg cgacaacgag aaacgccagc ggcgtgtatt tttcctgact taagatgttt
     9421 tgtaaaacgg atcggattct tttcatgacg gcgtgattat actttcactc ccgcctcaac
     9481 cctgaccttg aacctgtctc tgtttcactg gcctttcccc cgcctttgca gtaaaataga
     9541 acactcgttc ccctaatctt caagacccca tataccccaa aaaaacatgg acttcaaact
     9601 aaccgctccc tttatcccca tgggtgatca acccgaagcc atccgcggac ttgtggatgg
     9661 tgtgcgcaaa ggcatgaaac accaggttct gctcggcgcc actggcacgg gtaaaacctt
     9721 cacgatcgcc tccgtcattc aggaattgca gaaacctgcc cttatcatgg cgcacaacaa
     9781 gaccctcgcg gcacagttgt acgccgagtt caaggaattc ttccctgaaa acgcggtgtc
     9841 gtattttgtt tcgtactacg attactacca gcctgaggct tacgtcccgc gccatgacct
     9901 gttcatcgaa aaggaaaccc agatcaacga agagatcgag cgcctgcgcc ttgcagccac
     9961 cactgcattg gtctcccgcc gcgatgtcat tattgtggct tcggtctcat gtatttatgg
    10021 tttggggaat cctgaagagt tcaacaaggg ttctgtgaat ttggcagtgg ggcagttggt
    10081 ccgcagaaat gcattgctgc gccgtttgat cgaatcgcag tatcagcgca acgacatgga
    10141 actgcgtaca ggcaccttcc gcgtgcgcgg cgatacgctg gagatcatcc ctgcttatga
    10201 ggaaaaacgt gcctaccgca tcaccttctt tggtgatgaa gttgaacgta tcatgcagtt
    10261 cagcccgctg acaggcgaag tgttggaaga gccaagggaa atttccattt atcccgccaa
    10321 gcaatatctt accgacgccg aaaagaccaa agaagcgatg gcggatattg aatcggagtt
    10381 ggaggaacga ctggcgcaat acaaacaggc tggccgaata ctcgaggcac agcggcttga
    10441 gcaacgcacc cgctacgacc tggaaatgct ccgcgaagtt gggtattgct caggaattga
    10501 aaactattcc cgtcacttcg atcgccgtgc cgctggcacc cacccgtgga caatgatcga
    10561 ctttctgcca agtgattatc tgctggtgtt ggacgagagt cacatgaccg tgccgcagat
    10621 ccgcggcatg tacaacggtg accgcgcacg caaggaaact ttggtggagt acggcttccg
    10681 cctgccctcc gcaatggata accgtccgct caagtttgat gaatttgaag aggtgatggg
    10741 ttcaacgatc tacacatcgg ccacgccttc agcttacgaa atggatcacg ctgagcaggt
    10801 tgttgagcag atcattcgcc ccactggttt gatcgacccc gaagtggatg tccgccccac
    10861 tcgagggcag gtggatgacc tggtcggaga gatcaagcaa cgggtcgagc gacgcgaacg
    10921 tattctggtc acgaccctca cgaagcgcat ggcagaggat ctgaccacgt acatgaaaga
    10981 actgggaatc aaagtccagt atttgcattc ggaagtggaa acgttggagc gtgtgagcat
    11041 tctgcgtgac ctgcgcctgg ggaccttcga tgtgctggtg ggaatcaact tgttgcgcga
    11101 gggccttgac ctgcccgaag tttcactggt ggcagtgttg gatgccgaca aggaagggtt
    11161 tctgcgttca gacacggcgt tgatccagac catcgggcgt gcggcccgta atgtcaacgg
    11221 gcgcgttatt atgtacgcag accgtatgac cgattcaatg aagcgcgcca tcgacgagac
    11281 gaatcgccgc cgtgccaagc aaatgaaata caacgaagaa aatggtattg tgccgatcag
    11341 tattcacaaa gaaattcatg acctgaccga ggaaatgtcg gcaaaggcag ttggcgaaat
    11401 gaagggtgag tacaaagtga aaggttcgca gggcatgccg cgcggcgaac ttcggaagat
    11461 cgttgacgaa gtggaaaagc aaatgaagga agccgcaaag aaccttgaat tcgaaagagc
    11521 cgcggcgttg cgtgatgaac tgtatgagtt gaaaaccctg ctggctgaag atgagagctt
    11581 gaaaccgtgg gagcgtatca aactgatgac tggtgaggac gattagttct ggaaataaaa
    11641 ccagccgcct cgttacaacg ggacggctgg tttttgtttt agtcagcagg cctaacgggt
    11701 cggctttggt ttgtgatgga tacaggggta aagttctcca tccacgatca aaatgtgatt
    11761 gacgatccag tctgatattt cttcgtgaat tttcaaggcc atttcattcg aaccgcccga
    11821 tttggtgaat tcggcatggt acttatcgaa tttctcaaca aacaccgcat gagcatgttt
    11881 attcttttcg gcggcagggc aatgataggt ttccatgcag tcttcttctt ttccgaagtg
    11941 ccagcctgca taaaaccgta aaacacccag tattctggcg agttcctcgc cgccgtgacc
    12001 ttcgttgatc acgtcgccaa gcttgttgag ggtgtcaacc agatagcggt gctgactgtc
    12061 gagagaagcg tccccggtgc tgagtgcgtt ggtccattcc agatgtcttg gtgagtacat
    12121 tttgcctcca tggcttcatg taacaaggat tataaataca ttctggttta cataccttgc
    12181 aagctctgct taattttgtt tggttccagc ttatcctgaa cctctggcta tcagaaaagt
    12241 aaatgtcagg cctggtcgtt gccttcctct tcgtcatcgg gttgaatatc gtcaaatggc
    12301 tggagaagtt tgagcgccct tttcacttca tccttgggga tgaagatatc cacacgtgcg
    12361 gtggcaaggg tgtaggcagt acgagaaatg gattcctcga ccagttccac gtcgattccc
    12421 tcggcttcaa gaaagctctc gataacttcc gcttctgcgc ggctgttggc ttcaccgatc
    12481 ttttcgtagg ttagctcgtc cattttggcg gtctcctgat taaagtatat gccaatttag
    12541 ctgttttgtt tcatcgaaag cgcacgtcct atgatcctgg cagatgtgtt tgcggcggcc
    12601 gtaaacccaa agaacggacc aaaatcctgt gcggcgggca gacctgtaaa atacaggttc
    12661 ggaacgctgc tttggaagga ttcgtccaat acggggaatc cgttttgggt ttttattttg
    12721 gcggccaagg ttccgttggc gagcatgggg atttgcgcaa tgtccatttt gtagcccgtg
    12781 gcaaggatga catggtcaat ggggaaggtt gaaccgtcat tcagggtaat ttgcaattct
    12841 cccgcaggca gttctttgca gtcggctacc tgagtttgag gatgcaggtg gatggtctcc
    12901 tgagtgatac gtttggcgag ccatggctcc agtttcagcc tgccttcgcc ccacatccgt
    12961 ttgatgatgg cgtctttttc ttcctgtgcg agattgcgga accattcggg gcggtcagcc
    13021 atggattcaa ccagtggatt gacccatgtc cagtctgagg gggtgaaggc gggtgtgtcg
    13081 tggcggtagg tcatgtgaat gttctgtgcg ccctcctcgt ggatcaaggc tgcccattca
    13141 aaggcgctct gcctgccgcc aacaataagc actcgtttgc ctttcaggtt gctaaagtct
    13201 atatattcgg cagtgtgatg aaacctgcca gaagggaata gcgaattcaa cggttcgggg
    13261 atgtttttga aataacgcaa gccgaccgcc atgacgagct tttctgcgag tgtcgaactg
    13321 ccatctgcaa aagtgacgtc aaagccattg ccggcctggt cgattttcgt gatccatgcg
    13381 ggtagtgggg aaatgccttt ttgacgctga aaccattcga tgtaggtcag gtaaaagtcg
    13441 aggctgagcg gttccacatc cgctggttga aggttgagcg ttttcaaata gttgacaatg
    13501 gtggcttcgt tgtgcggatc aaggtgccag tcgcaggcgg agcgaaggaa cattttgagg
    13561 ggcatgaaat tcttccaata gtccatgctt gcgccaacgt gaatgtgttc gatcttttca
    13621 tgctttaaat aggctgccag cgagaggcca aatgggccag cgcctgccaa aagtaaaggg
    13681 gtgttttttt gcatatctgc ctccgtttgg gacgattttt tcagagataa ggcttattgt
    13741 actctttgtg atttctttat agagtcttta cattggggtt gtatcctgtg gttgtaatca
    13801 aatcaaataa gcggaaaaat tccgcagaaa acaggagata aaaatgaaaa agacaatttt
    13861 gatcgtaggt ttgctggttt tggcgctggg cgtgttggga gtaggtgtgg tctttgcgca
    13921 agggacgaac cctccgtacg cgggtcacgg cccgatgcga aacggtgaag gccctcttca
    13981 taccttcatg gtgatcgagt tcgccaagaa gcttgatctg aacgtgaacg atatcaacac
    14041 ccgcattgcc gctggcgaga ccatgtacga cattgccctt tcggctggtg taactgccga
    14101 agaattcccc acagtcatga cggaagtccg cacggctgcg ctggatgcgg ctgtgaaagc
    14161 caatgtcatt acgcaggaac aggccgactg gatgaagtct cgtggatttg gacagggcgg
    14221 catgggaact ggtgcctgtg acggcactgg catgcagggt gctcaaggac agggcatggg
    14281 gcgcggtatg atgggtcagg gacgcggctg gcagaaccaa caggttgctc cgtaatacat
    14341 cgaataaatc aaaaaggagt cggctgcggc tgactccttt ttgattcttg ttggcttgga
    14401 gcttattctt ttcctgtcag taaatacttt tgcacatcgc cgcctgtgaa gattccgtct
    14461 gtgacaactt cgtagccgcc gagctctttg atctcatcgt acatggctga ggctttcgca
    14521 agcagcgagc ggaagatgta tggaccgaag ctgacgcggt tgatcccgat ctcctgtaat
    14581 tgtttcaggc tggcggtctt tgcagaggcg aggacgttga tcggggcgtg gatgcgctgg
    14641 cgcaggatct tgagcgtttt ttcgtcacca gcgccgatgg ggtagatgca atttgcgcct
    14701 gctttcaggt aggcatttgc gcgggtgaca gcatcttcga tgcgctcttc tgtggaggca
    14761 aaaccctctg caaagaagct gtctgtgcgg gcgttgatga cgagggagat tccgcgtttt
    14821 tcagcagttt ctcggatggc ttgaatgcgt tcgacctgtt caggaatggg gcggagacgg
    14881 gcgccgtcat cccagctgtc ttcaaagttg atgcccacag cccctgtatc aagcagattg
    14941 gaaatgtttt gctgtaatcc cgccacggtt tcggagtaac ctgcttcaaa atcagcagtg
    15001 acagggacct ctacgctgtt tgcgatcctc ctgaccattt ccagcatcga ttccagtttg
    15061 agttgttcgc catcgttgta gccgagagat tcggcaatgg cggcgctggc ggttgcgacg
    15121 gctggaaagc cttttacctc caacatccgc gcgccgatgg gattccatac gttggggagg
    15181 atcagtaatt tgccgttgtt gtgcaatgca agcagggatt gagctttggc ggcctgggtc
    15241 atggggtctc cttaatggtg ggcgtggtga tgttcggcga tgctgcaaaa caggtcgctg
    15301 gattgacagc cttcacattc cagttgcagt gtggcgtgtt ggatgtggta ttcgtgggca
    15361 agcatttcgt tgatgttgtt ttgaatgaag gttccatcgc ggagtgatat ctcatcggtc
    15421 actacatggg cggaaagcat gcgcaggcct tcgctgatgc tccagacgtg caggtcgtgt
    15481 acatcgcgca caccatggac tttgcgaatg tctgagatca tggtcggcat gtcgatgttt
    15541 tcgggggtgc tttccaaaag gatgtggatg gtctgccgta aaattcccca agcgttccag
    15601 acaatgaaag caccgatcaa aacgctgaca aatgggtcga gccagttcca ttttgtgaag
    15661 tagataacca cccctgcaat gaccgcgcca agtgtggaaa tgacatcgcc catcaagtgc
    15721 aggaatgcac tgcgcaggtt gagatcgtgc tcacttcctt tttgcacgag ccaggcggtg
    15781 acggcgttga tgaaaaatgc cagggcaccg actcccatca gaatcgctga atcaacttcg
    15841 ggcggggaaa ggaagcgctg ccaggcttcg tagaaaatcc caagggcgat caatatcaaa
    15901 gtggtggaat tgaccagcgc aacgagaatt ccgactcggt gatagccgaa ggttttgcct
    15961 gcattggcgg gtttgagcgc gatcttgagc gcgtaccaac tcaagccaag agcgatgacg
    16021 tcggtgaagt tatgggcggc atcggtgagc agggcaaggc tgttgccgaa aatcccagcc
    16081 gccacctcaa cgaggacgaa agcggcggtc agtgaaaggg aaagcgacaa tcgttttgta
    16141 gtttgcgtgg caaggtcgcc gaagtgtgaa tgactgtgcg aatgtgacat gctgtaatta
    16201 tagtagagac acggcagcgc cgtgtctctt tttttatgcc cagccaaaca atctggcaat
    16261 cgaggcggtg aggaaggtga ccagaaacgc tgtgcccaaa gtcatcacga cgctgagggt
    16321 ggcccatttg agcgacttgg tttctttgta gatcgtcatg atggtcgtgg cgcatggatt
    16381 gtgcagcagc gcgaacagca tcaggttgac tgcggtcagc attgtccagc cgttgccgtg
    16441 aacgagcaga tcgtaaatgg ctgtgtttga agaaggcccg tcgatcatca tgcctgctcc
    16501 catgtacatc atcatcatgg tcggcacaac gatttcgttg gcagggatgg cgatgatgta
    16561 tgccagcaga atgacaccat caagaccgag cagcaagcca aatggattga gccaatcggc
    16621 aatggcacgg gcgaggctgg tatcgccaat gtgaacgttt gccaaaatcc agatgattgc
    16681 gccagcaggg gcggcggttt gcatggcgcg ccacaacaca aaaatggtac ggtcgatgat
    16741 ggatgtgtag aggatgcgcc caaagttcgg tttgcggtag ggtggcagtt caagcgtaaa
    16801 agcggatgct tcccctttga ggacggtgcg ggacaggatc cacgacatga ggaaggtgaa
    16861 cacaaccccg agtaacacca caccgaccac tgatcctgct gcgatgacgg atgaggtcaa
    16921 cccgctgaag cctgccgcca caaaaaccgt accaagcatg atgagtgtgg ggaagcgacc
    16981 gttgcatggg acgaagttgt ttgtgaggat ggcgatcaac cgttcacgag gagagtcgat
    17041 cacccgtgtt gcgatgacgc ctgccgcatt gcagccaaat cccattgcca tggtcagcga
    17101 ttgcttgcca tgcgctcccg cctttttgaa catccaatcc atgttgaaag ccacgcgggg
    17161 aagatagccg aggtcttcaa ggaaggtaaa ggctgggaag aaaatcgcca ttggcgggag
    17221 catcacactc accacccagg ccaatccgag atagacacca tcccaaatga agcctgtgat
    17281 ccaccagggg atgaatccca gtgaattgaa aaaattgcgc ccgatttctc caaaagcgaa
    17341 caaaacattt gcaatggcat cggaggctac gtttgcgcct gaaacggtca gccagatgat
    17401 cagggaaagc agaatcaaca tgatcggcag gcccgtgatc ggcgaggtga cgaggcggtc
    17461 tattttttgg tcgaggtcat agcgtttgtt gtccgcagtt tttacggcgc gttttgcaat
    17521 ggcctctgct tcagagtaca gcgatttgac gatctcatcg cgaaaccccg atgagagatt
    17581 gttacggagt gattccgctt ttgagagaat gtcatttggt tttaattcag tagccatatt
    17641 actgatttcc ttccacagac attttcctgc tgaactgcac gtccatgcgc tgctggcgcg
    17701 agacgatatc ggccagttcg ccgctggtca atgctttttg tacgcgggca tctccatcaa
    17761 gcaagcggat ggccagccag cgtgcattcg gtattcctgg tgcaacctgt tcgatcatgg
    17821 gcaccagttc gtttacggct ttttgaaact cgggtgtgcc gttcacgcgc aagggtttga
    17881 tctcgacctt gccttcgatc acttcagcta caacgccgag cagggtgtgt atgccttccc
    17941 cggtgcgggc cgtgatcgga atcgcaggca cgccaaggtc acggctgaga gaacgtgtat
    18001 ccacttcaag acctttgcgg cgggcttcgt ccatcaaatt gaccgcaacg acaacgttct
    18061 cggtgatttc catgacctgc atgacaaggt ttaagtttct ttccaacgcg gtggcgtcag
    18121 ttacaacgat cgtgcagttt ggctggccga acagaatgaa atcacgggcc acttcctcat
    18181 cctgcgaggc agataaaagc gagtatgtgc ctggcaggtc cactagtttg tagcgcatgc
    18241 ggttgaattc aaaaccgccc tctgcacggg tgacggtctt tccaggccag ttgcctgtgt
    18301 gctgtttgag ccctgtcagc gcattgaata aagtggattt gcccgtgttc gggttgcccg
    18361 caagtgcgat cacgcggtcg aaatctccca ctttaattcc catttgttcc aactgtgtaa
    18421 aggcggggca ggtttcgcaa tgttctgtcg ggagtacagt ttttgtgttt gtcatggttt
    18481 ctcttttcta aaatatgcac ttcttaaagc ggcttcaccc agatctgtgc agcctgatct
    18541 ttgcgcaatg cgatcaaagt gccgcggatg cgataggcgc gcggatcgcc gaaaaaattt
    18601 tttaattcag ggtagatcag tgtgccgggg gtcagaccaa ggtccaggaa tctgcggcgc
    18661 gtaaaacctt gcacagcatc atcgagcatc acgatctcgc cgcgttggtc gtgtgcaagc
    18721 tctgccagcg gcacggcttc acttttcagc gattcgatct ctggcagtgc ggtcacatgc
    18781 acattcgaag ccacggcagg tgccagtttg tactcggtct cgccgtcact cagcagatag
    18841 cggccttgtg ttttttcaat aatgcggatg acttgtccca ggcggagccc tgcggccaga
    18901 atctgttgat acgcaatggc aggttcatct tcaagatgta cgattcttgc gggacgatcg
    18961 gcctgccagg ccgtaatggg cattccctcc gccttaggca gagtcccctc acgagtcgga
    19021 atggggtcgc cgtgtgggtc gcgggtcggg tggccgagtg cagcatcaag ctcgttgagt
    19081 tgggcatcgg tcagggtatg ttcgcggcgc tgggcttcat cgtggacttt ctccaaaggc
    19141 attcgcgctt catctgcgag aaatctctcc caaagcctgt gcgcgcggac gacatgcaag
    19201 gcccagcgtt ctcctttgga ggtcagatgc aggtcattgc cgcgggtttc aagcaattcc
    19261 tgtgtttcca tattttcgat cagctgggtg gtttttgcca gcggcagatc aagtgtccct
    19321 gcaagagaaa cgggggaggc gtgtcgtccc tgttgttcac ggtcaagcag atgcttgaga
    19381 gcatcttcca cgtgttcacg ttctttggct tcgcggtagg ttttgtacac ggcgatcaac
    19441 cccacgcgcg gtagaaagat caacgcgctc aaggcagccc caaggatcaa aaggatgatc
    19501 ggattcatga tggtttacct ccaagctttg gtagaaaacg acctgtggtt ttcatatatt
    19561 cacgatattc atcgccgaat tttgcaatga ggttggcctc ctcttttggc gtacgaattg
    19621 ccatgacaat gaatgccaga actcccaaaa gagcgatgaa ccaattgtcg gccatcaatc
    19681 caaacgaaat gaataacgat gtcccaactg tgtacagcgg atgccgaacc cagcgatatg
    19741 gtccgcttgt ggaaagttta tgttccttgc gggtggcgct ggcaggggaa atgccgtcgc
    19801 caatgctgct aaaaagccaa taaattagtc cgtcactcgc aacaccaagc cccaccccaa
    19861 gccagcgcgc ccattcgggc aggccgatct ttgaccaagc catccattgc ggggcgatca
    19921 ggtacacaaa cgggcttagc cataaaagca ggccaaacag tttgatgatt cccatcatga
    19981 cagatccatc tgttttgcgg gaaaccttct cgcctgtgtg acggtcggct ttgatgcggt
    20041 gataacttga gatggccatg ccgctgatca atacaattgc ggctatccct cggaaaatgg
    20101 tttcattcat atttttttac ctctatttct aaatggtcaa atttctttca cgtagatctt
    20161 gcttgtaatg ttcaacccaa gcacaatgga ttctttttcg atctggatgg tcaggttgtg
    20221 gtcgtaggga gagtacgcct tgattttgat ttcagcccca gggaccagtc ccagattctc
    20281 gagataacgc agcaggtcgg tatctgctgc tttgacacat tgaatggcaa cgatacattc
    20341 tggccgcagt gaagagagcg gagtcgaatc ttcaaagggc atttcaagat cggcggtggg
    20401 gatcagctct ccgtgtgggt cacgaagcgg gtgtcccatg gcggcggcga tccgcctttc
    20461 gaaatcctca gaaatgacat gctcaagctt ttcggcttct tcatggacct catcccatgt
    20521 gtacccgagg gtttgaacca gccacgcttc caacaagcgg tgatgacgaa tgacttccaa
    20581 tgcggctttt ttgccgtcat cggtcagggt gacgccctgg tgtttttggt aggtcacaag
    20641 ggcgggtttg gctgaagcaa gtttttgcac catgccagtt acagaagctg gcttgacctt
    20701 gagtttggcg gccagtgcat ttgtgctggc gctggtgccg ttttcagtga gttcataaat
    20761 gtgcttgaga taatcttgaa tggagatggt caggttttgt tccatgttat gcgttccaga
    20821 gtgtgatggt ggtgcaggtg aaaaagatga aatacgccac gttgaatccc gctccttcaa
    20881 gcgcgtctgt gataaaagca ggcaaaatac gcttggaaag cagccaggcg atggtcatgg
    20941 ttacggggtt gatcgccgcc atccatgcgg ggaacattgt gctttgtgtg gctactgcaa
    21001 cagaaaataa aacagatgcg atcacgacca taaccagcaa aggcgcatag gttacgatga
    21061 gtactttgcg gtgacgggta aataattcca caaaaatatt ttgcgaatct tcgctgacct
    21121 cgttcagggc tttgacatat tcacctgcgt aaaagaaaga cccatgtaca aaggcaccga
    21181 tcacgctgct gcagacaaat agcagggcag tggcagtttt gaggatcggg tttatatccg
    21241 ccagaccttg atagacctgc caaaacccag caagcaccaa tggcgtagag aagaccccga
    21301 tcagtgcgcc ccacatcatt ttttcagatg ggatggaaac gaacttctcg gcatctgaaa
    21361 gtaatttttg aaaaggtttt agtttgggat agtcgtcgat gttcaccttg ttcgcaagga
    21421 gtaaaacatc tccaatggca tacaaggttg cgccgacaat tgccaacaaa cctgtgactt
    21481 gtattgaatt cataaatact cctaaaatta gtcaagccta aatatatttt aggcttgact
    21541 aatttatttg tcaagggcag ggttgacttt tcgtgggtga atctgtacaa tgcccttagt
    21601 ttgtccattg aatttacaaa gtcctttgcc tggattttca cacggataac cattgaaaaa
    21661 agcgacgcat caacaaacga agcagcacaa tcgcgacctg gtcctgcgca ccatattttt
    21721 gcaggagagc atcagccgcg ctgatattgc acgcctcacg aacctgaccc gcaccacggt
    21781 ttcagatgta gtggctggtc tgatcgccga aggtttggtt tgtgaagtgg ggcatggtgc
    21841 ttccatcggc ggcaaatcgc cgatcctgct gagtgtgatc gccgatgcac ggtatgtcgt
    21901 tgggttgaat ttggcgcagg ataaattcat cggtgcgctt gtcaatttga agggggagat
    21961 caaggaaacc atcgaattgg atgtacatga aagcgatggt gaaaaaggcc tgcaatttgt
    22021 gtatcaaatc atggatcagc tcttcaaaaa gaaattgaaa cccatcattg gcattggcat
    22081 tggcacacca gggttggtaa acacccgtga gggtgtggtg ctcaacgcgg tcaatcttga
    22141 ttggcaggat ctgcctctgg gttctttgct tgaaaaaaaa tacaaggttc ccgtctcagt
    22201 attaaatgac agccaggcgg ctgccatagg agagtttgtg tatggcggcg atcatagacc
    22261 tgatgagaat ttgatcgttg tcaacgcacg gtatggcatt ggggctggca ttcttattaa
    22321 tggacgattg tttcaagggg atggcggcgg tgcaggtgag atcgggcatg tagtcgtgca
    22381 ggagaacggc gaaacctgtc ggtgtgggaa gcgcggctgc cttgaaaccg ttgccagtgt
    22441 ccgcgcggtg ttgcggcaaa tgaatgctga ttcgctggag gcggtgagtg aagcgtttct
    22501 cgctggtgag gcgcgcgcag tggatatcgt aaccaaggct gcgcattttc tcggtgtttc
    22561 ccttgccaac ctgatcggca ccttgaatat tcaaaagatc gtcctcactg gcgatatgac
    22621 ccgttttggc cctgcatggc tttctgaagt tgaggcgtcc atgcgtgcgg cggcattgac
    22681 tcgcatgtca ggtgccacca aacttgaact tggcaaactg gattatcgcg cctgcattct
    22741 gggcgctgca gcgtttctgt tgttagatga ttattcactt ttgttcaacg aggaaaactt
    22801 atgacctttc aattgatccc cccgcgtgtt gtcccgcctt tggatgaagg ttttcggcct
    22861 gccgtgctgg caaatcggtt tttttatcag gcggcaactt cccctttgct tttggggttg
    22921 gagcgtaatg gcggcgaagt ctcgcgtttt gaaacgaaga tcctcccgcc tgaccatcca
    22981 gattttgaag ataacttttt gtacgtcgaa cgcattgtca agtttttgct ttggcagcgc
    23041 ggcgggcata cattgcatgt cggcaactct ccacgggtgg ctgaatatct gcaaacgctt
    23101 tattccgcac agggtgagcg taaattcgat tgggagttca tgggtgagca agtgtatgag
    23161 cggcctttga aaattcttgc atgtgcattg gatgaagtgc ccgcttcgcg cgaaacgggg
    23221 aaactggtcg gccgtaattt gcaggggtgc cgcattgggt ttgatcttgg tgcctccgac
    23281 cgcaaagtca gcgccgtggt ggatggaaca cccatttaca gcgaagaggt catttgggag
    23341 ccgcgaaaaa attccgatcc cgaatatcac tatcgcgaaa ttgtgactgc catccgaacc
    23401 gccgccagca aaatgccccg cgtggatgcg atcggcggca gttcggctgg gatttacatc
    23461 gacaaccgcc caatggttgc ttcgttgttc cgcagtgttc cgcgtgaacg atttggcgag
    23521 atcaaggaga tgttttccaa aatccaaaaa gaaatgaatg tcccgttgga agttatcaac
    23581 gatggcgatg tgaccgcact ggcaggctcc atgtccattg aagatgacgg gattttgggc
    23641 attgccctcg gttccagtga ggctgcaggt tacgtgaata tggaaggtca tatcatgggg
    23701 tggctgaacg aactggcttt tgcgccgatc gattacagcc cgagcgcacc tgtagaagaa
    23761 tggtcgggtg acatgggttg cggttcctca tatttttcac agcagtgtgt atttcgcctt
    23821 gcgccaaaag ctggcattga cattccctcc gaagtgaccg atgccgagaa gttgaagttc
    23881 gtgcaggaaa aactggaggc agggcatgag ggcgctgtca acatctggcg gagtatgggc
    23941 atttaccttg gatatggcat tgcccactat gctgattttt acgacatgaa gcacgtgttg
    24001 attctgggac gctgcacctc aggccgcggc ggtgacctgc ttatcgaagg cgcaaagaag
    24061 gtcttcgagg ttgaattccc tgagttgttg cagaaagtgg aactgcattt gcccgatgag
    24121 aaaatccgcc gtgtggggca gtctgtggct gcggctagtt tgcctgtgat taattgatat
    24181 catttcagct atccaataaa ggagtcctat gaaatttcat cttgatactg ccgaaatctt
    24241 cgtccctgac cttgagcctg tggaaaaggc gctggctcgc acaacacacc tgtgccttgc
    24301 cgcgcatcaa gacgacattg agatcatggc tgcccagccg atcatcgaat gttttcagca
    24361 gaaggaaaaa tggttcacag gcgtggtcgt gaccgatggc cgcggttctc cgcgcaacag
    24421 cctgtacgaa aactacaccg acgatgaaat gcgccttgtc cgcttcaagg agcagcgcaa
    24481 agcggctgtg gtgggcgagt ttgccgcgca ggtgatgctt gatattccca gcaaggttat
    24541 caaggatgtc tcgcgcaccg aacctgtgga ggatattctc aagttgcttc gggcaaccag
    24601 acccagatac gtctacaccc ataaccttgc cgataaacac gatacacatg tgggagtggc
    24661 tctcagggtg atcgctgccc ttcgaatgct ggaccagtcc gaacgtccag aaagaatcgt
    24721 ggggtgtgag gtctggcgcg cactggattg gatggtcgat tccgacaagg tgttgatgga
    24781 tgtctccaat catgaaaatt tgcaggccgc tttgttgggt gtgttcgatt cgcagatcgt
    24841 ggggggcaaa cgatatgacc tcgcttcgat gggtcgccgc cgtgccaatg ccacgtattt
    24901 tgaatcgcac ggtgtggacg ccacaactgg cctttcctac gcaatggata tgaccccgtt
    24961 gatgaacgat gcaaagatgg atcctgcaga attcgcccag gaattcattc aacggttcgc
    25021 agtggatgtg aaaagccgca tcggcaggat gaacgcatga cactattttc tgaaatcaac
    25081 gaacagcctg aaagaatcaa ggcgcttttg gcttcgcagc ggaaaaacgt ggagcggatc
    25141 gctgctgaga tcaaaaaacg cgatatcgag tatgtgttcc ttgcagcacg cggtacctcc
    25201 gataatgcag ggcgctatgc aaactatttg cttggggcaa tgaacggtct tccgcttgcg
    25261 ctggctacgc cttccttgtt cacctattac aagcgcccgc cgaagttgaa aaatgcgctg
    25321 gtgatcggtg tgtcacaatc gggtaagtcc ccggatattg tcagcgtttt ggaggaaggc
    25381 agaaaacagg gttgccaaac cctttccatt accaatgagc ccgattcgcc gttggcgcag
    25441 gcctcggatt ttgttttgga tattcacgca ggcgctgaaa aagcggtggc ggcaacgaag
    25501 acctacacta cagagttgat gtctgtggcg atgctttctg cggcgttgag cggaaacaag
    25561 gctcagtgga atgacttgag caaggtggcg gcctggatga ggcaatcgct aaagcaaaat
    25621 gattttctcg ctcaagctgc gcagcgttac cgctacattg accagaccgt tgtccttggc
    25681 cgcggattca actatgccac tgcattcgag tgggcgttga aactcaaaga gttgacctac
    25741 atcattgcgg aaccatattc ctctgcggat tttgcgcacg gcccgatcgc gatggtggaa
    25801 agcggatatc ctgtttttgc agtggcgcca aaagggaagg tgttcaattc catgcttgaa
    25861 atgctcaacc gtctgcgctc ggatattgcc gccgagttga tcgtcatctc gaacgataag
    25921 cgggcattgg cactggcgca ggttccgtta agcattccag tggatgtgcc tgaatggctt
    25981 tcgccgttgg tgaatatttt gccagcacag ttgtttgcct atcatctgac ggtggcgaaa
    26041 ggctacaaca cagagcagcc gcgcagtatt cgtaaggtga ctgagacaaa gtaatgcaag
    26101 gacctggggg ggaggtcaac ccgtggaaga catgatgcat cttcggaaag aaaatttcga
    26161 gatgtgtcat gtcttttttt gtaaaaacat tgtatgaata ctggttctat atgataatga
    26221 caaaaaaaac gaagaggtga cctcatgcgt gtgtgtgtac tttcggatga aattatcgat
    26281 gacttcaacc catctcccta tttgaaagat tacgattggg aaatggtcac cctgaccgcg
    26341 cccgtggaag aaaaaatacg cgcgctctct gaaagtaaaa aatttgatgt ttttttgaac
    26401 gtctgcgagg ggtatgagtt tgaagatgaa gaggatcatg agcagggcta tcaggccatc
    26461 gaagtggtgg aagctctgga gaaactgggt ctgccgttta caggggctga ttcaaattgc
    26521 tttgacccga cccgtgaaga aatgcaggcc attgccgagg cgcatggcgt cagttttgtg
    26581 aaagggtacc atgttacggg tgaggatgaa gcgcgggaat tggtcaaaaa tctgcgctat
    26641 cccatcatgg tcaagcatcc aaaaagctat ggcagcacag gcatgacccg cgaatcgcgc
    26701 gccgagaccc ttgagcaggt tctggcgcag gtaaagcgga tctgttccga gtttggtgcg
    26761 gctcgcatgg aggagtttat tgtaggcaag gaatacaatg tgctggttgt tgaaaatgca
    26821 aatgacctca gccagccatt tgcctacccg cctgcagagt tggtcttccc tcccaatgaa
    26881 gatttctggc atgtggatgt gaaatggaat tacgatgtcc cctttgattt caatcaagtg
    26941 aaagaccctg cactcattga aaaactgcac gatattgcca agcggatgta tcttgcgatg
    27001 ggagtttctg gatatggccg ttgcgatatc cgcatgaatg atcagggaga gttgttcatt
    27061 cttgagatca accccaatgg cggcatcatg tttaagcccg aagaatacgg ccctgctgat
    27121 tacatgatcc tgtatgatcc agatggatac aaaggttttt ttgaccgaat atttcaggcg
    27181 gcattggctc gccagaaaat gcgttcaaaa aagtaacctt gagaaaaaaa tgaaaggcag
    27241 gcaaacgcaa tattgcaatg ttgcgtttgc ctgccttttt gaattgttgt tcaggctgtc
    27301 acgatcatgg cgctgtgaca accaaattgt cgaagtggac ttccaccgtt ggctcggctg
    27361 actcgaagga aacaactgaa aatgccatcg ggcctgattc ggggccgcca gagatggttg
    27421 ccgctgtggc tacctgttcg ccgttgactt ccaaaatcaa gctgttcccg tcacaagtgg
    27481 cgcggatgat gttatgtccg cttccttgaa cgatcgtgtc cgaataggtc cagtccacca
    27541 gcggttccat cgaacctgcc aggatgctac gaatggaata gtaaccgtct gcgctaatgg
    27601 caagaaggta cccgtcaatg cttgtgtctt cgcgggtttg cattctgcag atgatcccaa
    27661 tccctgcatt gttatttgct gggccaaccg tcatggaagc atctacttcg atgacggtgt
    27721 cgctgaattc cgagttggac ttaccccagt tccacacatc gccattggct cgcaaaatgt
    27781 acaccccatc cttgatctcg gatgagcccg tttcgtcact ggtcgtttcg atgcggctgc
    27841 taaattcgtt gaagtcatca ctcatcaaga tgttggtgtc ttccagaggc ggcagggttg
    27901 gaacttcgaa gccgtcttgt gttgggggga tggcttgagt gggtggcacc acaaagggtg
    27961 gcgtggtggg ttgggtattg attgtgggca ggctggtggc tggacaggca caggcaagca
    28021 caaaaatggc tgcaacactg ctgaccatga tgcgggtatt tttattcatc tcattctcct
    28081 tgggtttccc gcattatagg tgactcgaaa aataagccta agccttacgt tggtactgta
    28141 tctcccagag cgcgcgccag tttgcgccat catcatcgga gcgttcccag ttccatcgaa
    28201 attgattcgc tgagatatcg aaccagacca tgcgttgttt gatcggtttt ccttccgcga
    28261 tcccgttgcg gctgaggatc attttcccgt ccacgaattc accgacgaag tcgagataac
    28321 tgcctgagtt atccacccag gtctgatgcc aacgcgaatc ttccctgctg aaaacggata
    28381 cgctgatccc gatcaatcca tccccgttga aattttcctg aataacggcg ccatccagaa
    28441 tgctttccac gcgatttgtg ccatgcactc cttcgcccca ggtcacgtcc cattcaccaa
    28501 gccagaaatc gaattcgtgg ttttccataa aactcctcca attgatttgg tttgtaattt
    28561 ttcgagtgta ttgaattctt ttacatctga caacctacca gagaatgaga tttcaagtac
    28621 aacaggagcg aatataatgg gactaccagt ttataagtga gtgaggcctt atgcgagttt
    28681 gcatcttaag tgacgaagcc attgaagact acaatccatc tgtgtttttt cgtgcccatg
    28741 agtgggattt ttttacagta acaccgcccg tgagtgattt catcaggcag attgtggagc
    28801 aaaaaaacta cgatgtcttt ttgaatattt gcgaaggcgc agatgacaac gatgcagatt
    28861 cgggcttttc tgtagtgcag actttggaat cgctgggcgt gccttttact ggagccgatt
    28921 caaagttcta taaccccact cgtgaacaga tgcaaaccgc cgccgaagca cgcgggctga
    28981 attttgcccg cggtttcaac gccaggtctg tgcaagattt gagccaggcg catgcgttgc
    29041 ggtttccgtt gatcgtcaaa caccccaaca gttttggcag tgcaggtttg accccgcact
    29101 cacgcgtgga ctcgttcgaa gaattggaag aacagtttca gagaaatttt tccgagtacg
    29161 gatcggcgcg ggtggaggaa tttatcgaag gtcgggaggt ttcgtgtctg gtggccgata
    29221 atcccgacga tctggcgaat ccgtttgcat acctgcctgt tgaggtcaaa ttccctgttg
    29281 gcgaaacctt catgcacatg gaggtcaagt ggctgaattg ggataccttc atcgttcctt
    29341 tggagggtga aggccttgtc cgctcagtgc aggaagttag caggcagatg tttcacgtca
    29401 tgggcggaac agggtatgca cgggtggata tccgcgtgag gccaaatgga gagcttgtga
    29461 ttctggagat caaccccaat tgcggaatct tgtattacgg ccccgatgat cgcagcatgt
    29521 ctgacctgcc catttcgtgg gataaagacg gtcacgacgg atttctcgac cgcattttcc
    29581 gttctgccat tttgcggcgt gacctgcgtt ctggaaggga aaaataatac cgcttgtgtg
    29641 tgggttatct tttacgcggt cgcagccagc gtccgcccaa taccagagag atcaacacaa
    29701 aggcgatggc tgcgtatgaa aatgttgtat tgtccatgcc gatgccgatg acaagaaaca
    29761 ccaatcctag aatcaaaaag atggtcgaag ctctgcgctt gtttatcatt gatttctatc
    29821 ctttacctcg ttgaaatctg cgaaattccc attgaataac tctgacggct gatttttcaa
    29881 agtcatttcg aagaaaccca gcaggtacga attcacgatc tcaaccacac gtccgccgtt
    29941 gagcggacct ttcaacccaa gctgcggtgc gatgggggaa agcaggggca aatcgctgaa
    30001 gtcaaaatgc ttcgttccgg aaatgacgat aatgccgcga ttgtcaccca gattcggctg
    30061 gaactgattg aacaactggt tatttttgct gtcggttaca tcggcccagc cctgactgaa
    30121 cataaagaac gacggttgcg ataaaccatt ttgaagcacc tcttcagaaa cggggcgcat
    30181 gaatgggtcc atgccgagaa tggttttgca gcgtggatcg gttccgcaga attgaatcgc
    30241 cgctccgccg cctgtggaat gtccatacac accgactcgg cttaggtcca aggtttggaa
    30301 gaaaatactt tccgaatcct ggttctgacc tgcaaactgg tccagcgtaa aagagatatc
    30361 gccagcccat tgatttacca gcttccgtgc caccacttcg taatttgggt cattggcatc
    30421 ttcaggcagg gcttgtgggt tattgggggc aactgttcca tcaggaaaca ctgtgacaac
    30481 cgcgccgtag gtgtgctgca ctgcgatcac cacatacccg cggcttgcca attcgagtgc
    30541 ctgccccgtg ttctgggcgt tgaagccatt ccagccatgc gaaaacacaa tgatggggta
    30601 gcccgtatcc gttttggccg cttgcgcatt ttcataggca ggaacattca ccagcgcaag
    30661 atgatcgagg aaaaatgaag gcaggttgag gtaacccgaa atggcagggg cgaaggtctc
    30721 cgcgttggac atccattgag cgcgctggtc tgatttttgg ggtgtggcag ggtaccaggc
    30781 ctgaaccata aaacggcggg gttcgctctt gtcgtcagaa tatagttctg tccgcgaagt
    30841 gtcggtgaat tcgtagatca ctgtgccaat tttgagtgac ccgctggggg tggggatctg
    30901 gggaacaggc agcagggcag ggattaccgt cgaaacggcg agcaggccca gggtgagtcc
    30961 gcgaccgatc ctttccttcc aaaccgaaga aaaagagggt gagtcaactt tggtgaattt
    31021 gatcaatccg cttgccgcga ggatcgggga taaaacgtaa agcgggatca tctgccagcg
    31081 atagccttca aacgtaaagt gaatcaaggt ggcggccagt gcaaatgcgg gcgcaaggcg
    31141 tatcaaaagc ggacgtggat tgggccagaa taaataaatg gcaagcagaa ttggaatgac
    31201 gatttcaaat ggacgcatgg aatttcctcg tgaatttatt atcccgtttt aagcgtggtt
    31261 gaatacaata aaacgcggag gcgagatgat tttattttct ctgagcattt ttatttcagc
    31321 ttttcttttg tttcagattc agccgatgat cgggaaattg attttaccgt ggttcggcgg
    31381 cacacctgcg gtgtggtcta cggccatgtt gttttttcaa gtgctgctca caggcggata
    31441 tgcctacgcc tactggctgg tgaaacgccc aaaacaggga tggattcatc tggccgtgct
    31501 gggcgggacg atcgcgattc tgacgaccct tgggctggtg tggtcgtcgc cgatcactcc
    31561 acctgcaaac atacactttg ccatgacggg cctgccagtg ttggatgttt ttttgattct
    31621 ggcggcctcg gtcggtttgc cgtattttgt gctggctgcc aatgggccct tgatgcaggc
    31681 atggtacagt cgcattttcc ctgagcggtc ttacgcgcgt ttgtatgcct tgtcgaatat
    31741 tgggtcgctg ttggggttgc tggcatatcc gctatttgtg gagcctgctt tttccgtgtt
    31801 ttaccaggga tggggttggg cgatcgtgtt tttcgttttt tcggttattg tgggaatctt
    31861 aagctggcga acccgcaacg agtctgaatt tggcaggaac gctctacgga ccgaaacacc
    31921 tgcgcttgcg ttgcatgtct tgtggattct tctgagtgga acagcctcgc tctttctgct
    31981 ttctgtgacc aaccaggttt cgcaggaggt ggcggtgatt ccctttttgt ggatcctgcc
    32041 gttgggggtg tatctgcttt cgttcgttct ggctttttct gattcgcggt ggtatgagcg
    32101 gcgtatctat gcattgctgt ttagtttggt ttcagtggca atgctctggg tgttgaccaa
    32161 aaccgaatct ttgaacgtgc tggttcagat cattgtgtat aacctgctgt tgtttctggc
    32221 ttgtatgatc tgccatggag agttgtaccg tttgcgccca caagccgatc acctgaccag
    32281 tttttacctg atggtttcgc ttggtggtgc ggcgggcggc ttgtttgtga atttggcagc
    32341 gccatatctt tttagcgggt attgggagtt ttatctgggt tggctgcttg tagtggtttt
    32401 tctggtggcg ctgttgtggc ctggtttgca ctcgcgcatg gaacggcagg cacaaataat
    32461 ggtcgctgca ttcgtgcttg gcgctgtctt gctggcgttg gggttgaatc gtgaggatgg
    32521 tccgcttttt gtggagagaa atttttacgg tgtgatccgt gtctttgatt gggatggcga
    32581 agccaattcc atggtccacg gtgtgaccgt gcatggattg caatttttag atgaccgttc
    32641 acgccccacc acgtattatg tggaggatag cggtgtgggg ctgctcctgc tcaaccatcc
    32701 ccgccgtggg catggtctgc gggtgggggt gctgggtttg ggtgcaggga ccctggctgc
    32761 ttatggagaa acgggcgacc tctatcggtt ttatgagatc aacccaacgg tggtgcaact
    32821 ggccgaagga gcaggcgggt atttttcttt tctgagcgac agcagggctg aagtggtaac
    32881 cgtgacaggt gacgcgcgaa tctctcttga acaggaattg gccaaaggtc agcggcagga
    32941 ttttgatgtc cttgttttgg atactttcag cagcgattcg atccccgttc atttggtgac
    33001 gcgtgaggca ttcgccttgt accttgagca ccttgcgaag gatggggtca ttgccgctca
    33061 tatttcaaac cgacaccttg atctgcgtcc gatcctttgg ttgatggcgc aggaatatga
    33121 cttgagcatg atccaaattg acagacctgc atcaggcggg gacaatggct ttccttcctc
    33181 ctgggtcttg ttggcacgcg attcgtcgct tctggagatt cctgcgatca aatcacattc
    33241 agccgatttc gattcttatg tcaccgcgat caaaccctgg acggatgatt acagtaattt
    33301 attccagata ctgaaataat agcgggtttg aagtttgcgt actatctaca caaaaagcag
    33361 gtggatgttg ctttttaaca acatccacct gctttgattt cacgatatta actgccgagt
    33421 ttattccgct ggtgctgggg cagaatcttc gggttgttga gccagttttt ccttaccgac
    33481 cttataagcc tgcgcgccca acacaaacat gagtatcgtg aggtgaaggg tcatgccaag
    33541 caggaggaac ggtaccagac aggcaaggat gagtgtggcg ccgaggatct ggatcaccaa
    33601 agtcacagcc atggatacca cataggtgat cgcaagggcg gcgatgaatc cgctcaggtt
    33661 ggcgcggaat atcggccacc attccttgaa gcggaggccc gccataaagt catctttttc
    33721 gatgacgtgc atttccgcac cgggaatgat caggcccagt ggaattgaca gcggaatgat
    33781 gcagcagagg ctggcaaata caagcaaaga gaaaaccgtg gcgacagatt cgatttccga
    33841 gctgttgacg gaattcatca cgatcggaaa tgcaattgac gaaatgatga gaggaatgag
    33901 cagaatgaga atcggagaga cgagaatcag gcgaatgcca aatactttca ccccgtcttt
    33961 gaccatgccg ccccagtcgt cccatgcgat catgcgaggc gactcgttgt tcataacctg
    34021 gcgagcgatg cgcacggcat aaccgaacaa aaccaaatac ggaacgatgg gaatgaagaa
    34081 tgccgccaat gcaaccagac agcctatcag gaaatatttg cgcgattcct ggtctttgaa
    34141 cgggaacatc aagatttctt tcaagtcaaa tccgaaaaac atggtcaatt cctcctgaaa
    34201 atattattgc agcgatttta taatgccacg cccatcacag cgacaaaatg cgcggcggcg
    34261 gccagcaaaa caaaaatatg ccagacttca tggaagccga acacacccgg tataaagttg
    34321 aatatttttg tagcatacac cacagcgcca agcgtataca ccacgccgcc gatgagcaac
    34381 cacacaaaga cccaggttgg cagggcggcg agcatttgtc cgcctgcaat gacgctgatc
    34441 caacccatca acacataaat ccccgcattc agccagcgcg gcgagcggat atagaagatc
    34501 ttcacgccaa taccgatcag ggccaacgac cagacgatgc tcatcatgcc ccatttccaa
    34561 aagcctgtaa aggcgttgat gcagaatggg gtgtaggtgc ctgcgatcag aagaaaaatg
    34621 gcggcatggt cgatcttgcg gaagatttcc agcgccttgt cttttacgcg caccatatga
    34681 taagtggcac tggctgagaa catggcgatc aggctgaccc cataaatggt cagcgaaatg
    34741 atcttggcag gtgtgctcca gccgacgatg agcagggcga tcaaacccgc cagggcaagg
    34801 atggctccac cccaatgggt gagactatta acaggttcac gtaatttttt caacataagg
    34861 ctttctccaa tttttgttcg gctgatttta cttgaattca gcgcggaaca aactacttgt
    34921 ttctgaattt gcttaaatcg atcaccaaag gtttgtgcgg tttgcctggc tttgtggaaa
    34981 aggcggacgc gattaagagc atgaaaactc caaaacaaca tcccgtcacc tgtgtactgt
    35041 attcaagaaa ataagccagc tttaccttcg caaattttgg aaagggggca aagtatctca
    35101 cttgataatt tccataatcg gcgtacgacc tgccgctgag gaagggatca aagtcaaata
    35161 acatgggggc ggtcagtggg tggaatgtta cctgttcgcc ctgctcggtt gtgaaatcta
    35221 ccctccctgt ccagcaggtt tttcctggcg ggcagttgac cgccgcattc gacaccttgc
    35281 cgatggcttt ttgcccaacg agatcaagca tcaccgcgcc agcgcaggaa aacagggtga
    35341 atagaaaact gaggatgcca attatgcgga ttacgttatt cccgagcgct tcttttcgca
    35401 agacttgttg ttgttcgttt tccattttta agttcctttc aggctacttc taaagatggg
    35461 gaaatgacgg cctgccttta ttttttggca accaggatga ccactcggct ggattttacc
    35521 tctttgattt gtacttcggt tagaaaaccg atctgtgtaa atggtgcaac aagacgtttt
    35581 ttcaaattgt cgtcaagggc ttgcggattt tcaccagtga ctttgtaaag ccatttcaag
    35641 aatcggttcg agggaaatgc cgctggcacc accaccagtc tgcctgcggg tttgaggatg
    35701 cgtttgatct cggagagtgt tcgagggtca aaaatatatt ctgtgggaaa ggtggaaatg
    35761 acacaatcga aggatgcggt ttggaatggc agggtttggg caagtccgcg ggttagtttg
    35821 tgggttggcc caatgcgttt tctggcgagt tttcccatct gtgctgattc gtcaattgca
    35881 acaggatcaa gtttcaggtc caggaggaag cgttgcaaat gcccgggacc atgaccaagc
    35941 tcaaggatgc gagtcccttc gataaatggg agaatgcttc ggccccagtt tttccactgc
    36001 ccaaaagaaa cgacagcggc aacaaggtcg taggtgaagg cgaatggatg gtaaagcagg
    36061 tgaaagaaca cccgcatgaa atttttcata agcatggata aaaaaagggt ttgactgcgc
    36121 agtcaaaccc ttttcatttt gcaaatgctt tagcctgcgg cggcttcgct tccgtcagcc
    36181 tttttgccag ccttgctgcg gacagcttcg agggcacctt tgccgcgttc gcggacttca
    36241 ctggccaatt cggcggcgcg ggcgcgggct tccttgctca gttcttcggc gcgggcgcgg
    36301 gcatcagcag caagctgttc ggcgcgggcg taggcttctt cagcagacat ctgagccttg
    36361 tcacgcagtt cgattgactt gtctttgatc agggcgcggg tttcttcgcc agattgcgga
    36421 gcgaagagca gggcaacgac ggcgccagta aggccgccaa ctacgaaacc tacgagaaaa
    36481 gctccaaatt catcacggtc agacatggga atactccttt gttgattggt tagttgtttt
    36541 tttatttggg cttgatgccc attaattcta acatacgctt caaaccagcc agatagccat
    36601 ttagtttgat aacaggctcc accacgtttt cgccaaggaa ttccgctgtg ccgcgcaggg
    36661 tgttgacggt ttcgctggtg gcatgcagaa tggggcgcac ttcattttgt aacaggttga
    36721 tgaggctggc caactggacg atgaggacga ccagtgccac accgatgacc agtgtttcaa
    36781 gggccacgac gatgatgaag acatcgcgga tcttgtctgt gggggtgttg ggttgaagca
    36841 ggaagtagat cgccacgcca agcagggcga caagcaatac aatgacggca acaatgccgt
    36901 acatcatttg tttttgcttg cgttcctgtt cacggatttc agccagttgt tcaggagtta
    36961 ggggaggggg agttggttgt tcagtggggg gaagcgttgc catgttttta ttatagcaga
    37021 ttcaagattc gaaaaaaagc caattggaaa cacatccatt caggcgaggt tcttttccat
    37081 gccgaggctg ctccaggaaa gcaaagagac ccaccagggt gatttcgata tctgtttgta
    37141 gcctaatttt tgatagaacg gaactgcgga tatattgtct tcgagtacct gcagtcggga
    37201 ttttttgacc ccatgctcgc agattctctc ttcaaggtgg gtcatcaact gcactccgat
    37261 cccttttccg cgcaggtttt gatctacaac aacgctgtgg atgagggcgc tttgcatgtc
    37321 ttcgtgtttt tgcacaccga tgagactccg cgccacggtc aggaacaatt tcaaagctgc
    37381 aggtgtgccc agcagccgaa atgcattcga aaccgttcca tctggggcgc gttcaaaggt
    37441 tggctcatca ctgaactgga tcatgcctgt tgcgaggatg ttcccgtttt cgtcgcacat
    37501 cacatagcgg tttttcagcg cattttcccc gttcgcttca agggtgaatc gaaaaagtgc
    37561 ttcctgtccg tcgatgggac ctgtcaacgc accgggtaat tcggcggcgt agagttgtat
    37621 taccagccgt gaaagggcta aagtgtcagc ttgaacagcg gggcgaatgg aaaaagaatt
    37681 gggcatggtt actgtccaag gatgcggctg agttcgagca catcaccagt gcgggcgcgc
    37741 agattgcggg tgagcatgtg gatcgttccc atcgctactt ccacatagtc gctcatgatc
    37801 tcgtaaaagg gtgtctggtc aaggcgcagg agcgaggtct cttccaaagc gcggactgtg
    37861 gctgagcgcg gcgaagaatc gagcagtgaa agctcgccga aaaccgcccg cgcccccaac
    37921 tgatttaaga cacggttgtc gtccaccact tccatttttc cgtccacgat aatgtacagg
    37981 ctttcgcctg tttctccctc cttgataaca gtttcaccag gcatcacact catctcttgc
    38041 aggagatcgg ccaattctgc gagcgcttca tcgggtgtat ctgcgaacat gcgtagtgat
    38101 tttaggataa tgactcgttc gacggtggat agcatatttt tctccgttct cttttgcctg
    38161 gcaatacgga cgatcaacga ttcttttgaa cccgtcaggg attctatgat cacctgactt
    38221 tccgcaattc ccacttcgcg ggcgatctcg atcgcgtttg caaccagcca tgggttttca
    38281 tgtgcaaggg cgttggtgag gatgtcaatg attcggctgg tgcgtggttt atcctgtttg
    38341 tagcgcgttc ccattttttg cagtcgctct gcagcgggga agttttccag gagtgggatg
    38401 aatgcagctt tatgagattg tgaaagtgtg gcatccatca cttcgatggc gtaggcggtt
    38461 cggttttcat cattgcgcgc gatcgctgcg cgggcacgat ggataatgtg cgggtcatgt
    38521 aaaaaaccga aaagataaaa caagcgatcg cgcgtttcgg ccaagtcatc gttgaaagcg
    38581 cggatgatgg cttccgtctt tggggtggat tgcaaatccc ccagacagga taacaaccat
    38641 gcggcgcgtc ttaactcaac ctcaatctga ttctgcacga tctgcatgga tcgcccctcg
    38701 ggcttgtatc tacactcgtg caaagcagtc aacacccgtg agcgcaagct gacattgtgg
    38761 tggttgatcc agctttcgag gacatgaacc gcatcgcctt tgatgtgcgc acaggcgcgg
    38821 accaatctca ggtggatgcg gcgatgcagg ctttcatcct tgatgctgcg gatgatctct
    38881 ggcagggcgg tttctcttcc tgcaaccaga gcgttgaatg ccagactgcg ggtttggggt
    38941 gaggcaaggg ctgcgatcac cagcggataa acatctggat gatgtgtttt tgcggcggca
    39001 cgtaacgcag ctttttttac ttcaatggat tcgtcttgta aaagcggagt caagacctcg
    39061 accatttgtg ggtttgcgat ctcaaccaaa agattggcgg cttgcagctt ttcatgagca
    39121 tgttccgacc ctgcaagtga cctcaggtgt tgatatgcct cctgcacctc gttgattttt
    39181 cgtgtcctga gaaggccaac tacaacgccg tgtgaagtgt tcgtgtcggg gctgttcacc
    39241 aatttcaagg catcttttgc attcttcgac aaggcagacc acgtgcaagc tgcgatctgc
    39301 cgtacgttgg gaacggcatc tttttggaac aaaacttcga tctcgctgag ggcttcattg
    39361 attttcaatc gttcaatgct tttcgcggcg tagatccgca cctcgggata gggagaccga
    39421 agttgttgaa tgagaatctc agtgaagttt tcactaccgc ttttttcaag caattccaat
    39481 gcgtacagtg cttcatttgg atgcgggctt ttcaatgcgt tgatgacgat ttctttgctc
    39541 accaggtcgg caagtggaat gcctttatct ccaaagcgat gtttgtgcag ggcttcggtc
    39601 aatgcattgg ggtaggcgcg gatcaaagcg aggcagatgc cgatccaaat tgcgccggag
    39661 agaagataaa tgaatgaaag ttggatcgcg tcgaatttta gcatggtgtt gaacagcagg
    39721 agcaataggc ctgccagacc aatggcaagc ggctgaacga tcccttctgt aatggtttgg
    39781 gcgcgaatac ggtcactttc ggcgatgggt tgatacaaca acgccgaagc cgtttggtcg
    39841 agagagaagc cgaccccttc gttggcaaat ttccctgcca cggccagcca aaagagaaca
    39901 aggataaggt ttgggttgat gctgcccgtc acggccaggg cggcagtgca taaaactgtc
    39961 agcgctgggg tggccaatag acctgcacgc aggccaaagc gtgagatgat tcgccccgtc
    40021 aaaaacatgt cggtgacgaa accgagcaat cccaccagtc caaagaatcc gccgataaac
    40081 cccgccagtt cggctgtgga ggggtatcgc aatgcggcgc ggtcataaaa aatattatcg
    40141 agaatgaaat atgaaacgcg ccaggtcgtg atgagggcga aaatgagaaa gatgtatttg
    40201 ttgcgaaggt aatgcaggat ggatgttttt tgtgtttcgc cttcaggact ctcaggggtt
    40261 gcgtatgtgc gcggattctt ttttagtgtg atctgttgca gaacgaacga aatcagcacg
    40321 cagagcgcga tgacggtata caggttttct gtgccgatgg cgttgaccag cggtgtggtg
    40381 aatggtccca ttgccacgta ggcaagccat gagcctgcat tcattaatcc aaagatgcgt
    40441 tttccctgcc gcacatcgaa aaggttgcct gccagagtcc aaaatgcggt aacggaaatg
    40501 ttgaccagtg tttgagacca gatgggcaga atgagcatca gccactttga agttgtgatg
    40561 gcaagcccga ttctcagggc gatcgtgccg aacaggagga aaagtatgga gagtatcaga
    40621 aattgcgcca atgaagtgcg cgcggaaatt ttcaaaaaca acgccgtgat ggatgaaaca
    40681 atgaccgcaa tgccaaggta aatataaggc attgcggttg tgtcccaggc tgtgaagaac
    40741 agggcgaatg aagctgattg aacgaacaac atggctgcgc ccatgaaaaa atactgggcg
    40801 agcatgagga agacagttcg ttcttcccct tcccgaaggt tgagggcggc gcggatgcgg
    40861 gaaatcataa aatggtttgc aaagtttacc agcgggattt tcctccgtcc atgatggggg
    40921 tggaatccaa aagtcccacg agatcatctc gtgggacttt tggatgggca ggtcttcaat
    40981 taccaaattc ggatgacctg gccgacatag atcttgttga tatctgtgat ttgtgggttg
    41041 agtacgatga gggagtccag ggtcgtgcca aaccgtgccg cgattttttt cattgtgtca
    41101 ccagattgga cggtgtagta ggaggcgctg gcaggcaggc tgatgacctg tccaacgtag
    41161 atcaaatttg catcggtgat ctgcgggttg accagcagaa tatcgttgac ggttacattg
    41221 atgcgggttg cgatcttttt caacgtgtcg ccttgttgaa cagtgtaggt tctgcctgcg
    41281 gcagggggtg ggacaggaac tgcaactccg ctgggtacgg tcatgcgctg gccctcgtag
    41341 atcaggttgt aattggcaat gctgtttaac gaagccagcg tgtccatgct tgtgccgaaa
    41401 cgggctgcca gtgatttcag cgtgtcacct cgtgagacaa cgtaagtagc gtagccattg
    41461 ccgttatccc aaaatgcacc aggtagtacc aggatctgtc cgacaaaaat gtatggattg
    41521 gctttggggt ttgcaagttg aagggccgca acggtggttc cacatgtttg cgcgatgctg
    41581 ctcaatgttt cgccgctctg cactgtgtag ctggttccgc agctataggc agacaccccg
    41641 cttgtgaatc ccagcgaaga gaccagtacg gcaagaagga taaagacttg tagaaatttt
    41701 ttgtgattca tgttacctct cattcattac agtagttcga tatgaatgag acgaaggcgg
    41761 ttgaaaaaag ttcccgaaat tcccttgact cttgaaaagt ggaggagtaa actttcccta
    41821 cctaccgttc ggtaggaaag gagaaatatg actcgagacg atgttcttga tgcagccgca
    41881 caggtttttc ggcaaaaggg ctttcatggg gcctccatgt ctgatatcgc atctgccttg
    41941 gatgtgcaaa aggccagttt gtatcatcat gtgacctcga agcaggagat attgctggcg
    42001 cttttggaac gtgccctggg aatgcttaca gaacatattg cctcgttcgc tgcccgaacc
    42061 attcctgccg atcaaaaatt acgcctgatg atccgcgggt atctttctgc cctcgccgaa
    42121 aatgccgacc tgacgtcggt tttgcttttt gagcatcgtt ctctcgataa aaagtctcat
    42181 actcgccatg ttccacaaag ggatcggttc gaagggttgt ggcatgatgt gctcgatgaa
    42241 ggagtacgtg aaaaggtttt tgatttgaaa gatacgggac tggctgtccg cgccattatg
    42301 ggtacattaa attggacgct cacctggtat cgtcccgatg gtcccaaatc catcgagcaa
    42361 attgctgatg agtattctga ctttattttg aagggtttgt tgaaatagtt cacggattgt
    42421 cataagccga aggaaaagga gtaagcaaat gtattcgttc gaacccaatg aagaacaaca
    42481 aatgttgatc gacgcggtgg ggaagttcgc ttccaacgac ctgcgtccag ccgcacatga
    42541 tgcggaagag agtcatgaaa tgtcacagaa gctcatcaac aaaggctggg agcttggcct
    42601 gctgcaagca tccacgcctg aagcgtatgg tggctttggt gaacgcagtg ctgtgaccac
    42661 cgtcctcgct gtggaagaaa tggccttcgg cgatctcgct ggcgctctcg ccgtgatgac
    42721 cccgtcgctg tttgccactc cca
//
LOCUS       DAOQJC010000018        41866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_393_length_41866_cov_9.938007, whole genome shotgun sequence.
ACCESSION   DAOQJC010000018 DAOQJC010000000
VERSION     DAOQJC010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 41866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41866
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_393_length_41866_cov_9.938007"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1324)
                     /locus_tag="PLE39_04260"
     CDS             complement(2..1324)
                     /locus_tag="PLE39_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1939 domain-containing protein"
                     /protein_id="HNE67568.1"
                     /translation="MQTFYWDCPREDKKEFQWWNYINREIPNLAKAGFTSLWLPPVHK
                     AANLGGPSMGYDPYDFYDLGEFDQKGSVPTWFGTRSELSQLIESAHRHGLSVIADMVL
                     NHNSGADLQEVNPITGQSRWTVFNPMSERFPRNWESFHPSMYESWDEMTFGDMPDLSH
                     RNPYVYAELLKLARWLIEEVGFDGFRYDFVKGYGANTITAIQEYRYMKNGAYHRPYGV
                     AEYWDNASNTLNWVNAANFSNSNPVDAFDFPLRELLKGLCDSYGFSLKSLVNGESILR
                     AQPQSTVTFVENHDLRDEGRPITNDKLLAYSYIFTHEGYPCVFWKDYFNYNLALRDTP
                     NGIDALISAHEKYAGGNVSTLYLSDDLYIMQRGGYENQPGLIYVLNNHGERWQGEWVN
                     TQWTNASFQPVAWWSKNDLSRPVNQSTGADGRGQFFAPPRGYAVYALK"
     gene            complement(1485..3155)
                     /locus_tag="PLE39_04265"
     CDS             complement(1485..3155)
                     /locus_tag="PLE39_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67569.1"
                     /translation="MNTLKLNRRPLILPLWSGLIGILPLTFLFDPPRASLKSLLELSF
                     VNLFTVGVLVFFIWFYFVRIKKELKYRIVFTSEAIEENQNKGFYSSRPRRILYSDVLS
                     VQYLFPGVIVVRSKNEEDFKFVLADIEGGADRVMELLKTHLPGTVIDEKLPEKMEKNS
                     ILVKINDAGMVLFLPLITIPVLGVYGFFQGGWNVHRLPWNEFLLDFSITSKNSPWIAS
                     TDFNGDNLIVHFDDQEQRWPLPLQNPRGTIILSDSNGQPTLITGEINYTWQDGKWLQW
                     KYFGVYKAAIYRKRFVSTTEQAWLVLYGDNKPNVLVGAIAGRPGVDVIQLPESAVQKG
                     YEPYSLSILADETVLVHAGNENEARIFLLKDGQWLEKSYDVPFDFLQVKDFIVDDVGR
                     LFIFYTTQTEEVESYFVQIQDDEKALITEIPLLGEFSDYDSLLVDSHDRLWILDDSYG
                     GGVTVLRPVWSGVAELVVQYNKENSNYPRSGRSSFVLDADGKVWVSSEDLYWIDSTAS
                     ELPRPMPDWLMDIGYSDTGYLKYIAILAGVFLGGYGILYLLSTKELKK"
     gene            complement(3213..3572)
                     /locus_tag="PLE39_04270"
     CDS             complement(3213..3572)
                     /locus_tag="PLE39_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNE67570.1"
                     /translation="MAVDLTQFEKEQYCYLTTRGRKTGDPHEIEIWFCVHEGALYLLS
                     GNMDRSDWVRNLLKDPNVTVRIAGQTFPAVAKLLDLKDGQTETLVRMKMAIKYNEWEG
                     RSPSEWARTALVVRVEV"
     gene            complement(3575..4132)
                     /locus_tag="PLE39_04275"
     CDS             complement(3575..4132)
                     /locus_tag="PLE39_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67571.1"
                     /translation="MTNSSRISSGFPPFQQGGLDSLCGLYSIVNAERFINHSSDEETQ
                     QLFDDLIHYLSRRGLLGKFLIGGILHHQMLLILNKVVGKKRISNVQIPWRGVPNPDLT
                     TFWHSMQAFLDGTPGRSIILGLEGYHDHWTVIEKITSRSILLYDSSLIKRLPRGRCTT
                     VYATEKRVHILLPAQTYFLSNEKES"
     gene            complement(4154..5308)
                     /locus_tag="PLE39_04280"
     CDS             complement(4154..5308)
                     /locus_tag="PLE39_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE67572.1"
                     /translation="MTSFLTDISSEMLVYLIPLFLSNVLGVRMAFIGLIDGIAETTAS
                     LLKVYSGALSDKLGQRKGLAVLGYAISTFAKPFLYFANSWGWVLGVRFSDRVGKGIRT
                     APRDALVAASTDETNRGLAFGLHRAGDTAGAFLGIAIAALIVWLTQQGALQITREAFQ
                     TAVLASIIPAVLAVIVLGVGAADVAASGKGAEKKRLSFKEIDPRFGKFLLITILFTLG
                     NSSDSFIILRAQERGLNVLQTLFMLMTFTAIYTILSGPLGALSDKVGRRRLIIGGWMA
                     YGLVYLGFAFAGAGWQIWALFGLYGIYYAATEGVAKAFVADLVPEAQRGAAYGWFNAA
                     IGITALPASLLAGLLWQGLGSWAGFGASAPFFFGAALALAAGILLWRLVE"
     gene            complement(5435..5887)
                     /locus_tag="PLE39_04285"
     CDS             complement(5435..5887)
                     /locus_tag="PLE39_04285"
                     /inference="COORDINATES: protein motif:HMM:NF015317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HNE67573.1"
                     /translation="MKTLLEKSKYLAIVGVLSLLIAAVAAFAWGTLKTAAAVLLVFES
                     MGKDAAITIEFIEIVDSFLIATAILIFAVSLYELFIAPLDLPEWMLAHNLYELKAKLS
                     SMIVLVMGVKFLQKLLEVKDTNDLLRTGVATALMSAVLIGFGYFGKKD"
     gene            complement(5898..6728)
                     /locus_tag="PLE39_04290"
     CDS             complement(5898..6728)
                     /locus_tag="PLE39_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE67574.1"
                     /translation="MHRERVSENVFWFQSEVYAQVTAGVIVGPQWAVVIDTLALPEET
                     LGMREFIEHELGVQVRYVINTHYHADHTWGNCFFPGATVIGHAKCRELLIEHGIPSLE
                     GARKQNPNLRQVKIIPPHLTFDSGELTMRVGKKNLVFSQSFGHSEDGISVLVEEDRVL
                     FAGDAFMSLPYIVDGDIDEMISSVKRIGRMGLENIIQGHGDIILRGEIDAAVKENMNY
                     LNNIKKLVKAAGKRKNADEYLDAITIEECGKSRVYLGGLAETLHRRNLRALYRQMYGE
                     "
     gene            6884..8587
                     /locus_tag="PLE39_04295"
     CDS             6884..8587
                     /locus_tag="PLE39_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNE67575.1"
                     /translation="MPADILVVTSSSQLGESIRRILEETDLYRIHVVTNKSSAIVRAD
                     EVGAPLAMLDLALGEGVIREIGQSLRTIRPGINLIILCDENITPPPFDELRPWILVRK
                     PFKMSNFMTAISQPQTTTATPPTPATTTSTPAAAPQNTSTPTLPWLSDPNRAAQHLTR
                     LTLESSAQAALITRKSDLWAYAGSLSQGAAKEVAQTITRNWDGQKGFDLLRFIRLEST
                     KAEHMLYATRLAADTVLALVFDAETPFSTIRAQASQLVTNLDNETAEPQKPSQPFNAE
                     KQVVEADADDGDAPNIPLISDILNNVPTPNPEPATTRDFNLPRKKETQEAFDPNQTRI
                     SESLSNASVFKRDPSPAVHYGSIPVLNKKPAPSIPEVQVQPQNQAVLNEVDVTAPSRI
                     QQRPETPVAKPAPGEQEVTRESPTTEAARKLVVEPTTAGLYHLTYACLLVPRFSAHYL
                     TGDLADQLGEWLPNICIAFGWRLEFLSVRPEYLQWVVNVQPNTSPGYLMRIMRQQTSE
                     KIFTDFTRLKRENPSGDFWAPGYLIMGGTQPHPQQLVRDYIRQTRQRQGQELPSEPKR
                     K"
     gene            8769..11561
                     /gene="polA"
                     /locus_tag="PLE39_04300"
     CDS             8769..11561
                     /gene="polA"
                     /locus_tag="PLE39_04300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNE67576.1"
                     /translation="MPPTLYLIDGHALAYRMYFALTAGGGSQRWQNSKGEPTAGIYGF
                     ARELVRVLEQEKPEYLAVAFDVGKTFRDDIFPEYKGTREKMPDDLRPQIERIRQMVDM
                     FNIPRLEMDGYEADDVLGSAARIAAKKGLGVKIITGDRDLLQLVNERTAVYLAGDDQT
                     YITDADVIKKLGVRPTQVVDYKAIVGDTSDNIPGVKGVGEKTAIALLEKFGTLDAIYA
                     NLDQVENRWKGKFEASKDAAYMSYKLAKIEVDLKIDLNLDHAAVRPFDSSALAAFFQE
                     MEFKTLLNKLPAISGSAPSVEIAAAPKKGKAGEQMSMFVNAPVIVSTPSKIEVTVVDT
                     DEKLGALKNELTRAKVISFDTETTSTEEVTAELVGISLAVKEGHGYYIPVGHVSGNNL
                     EIKKVIAALTPAMTDSKIGKVAHNAKYDYIMLARYGLVISPLTFDTMLAEFIVDPSSR
                     NLGLKNLSEYRLGEEMTHIEELIGKGKKQISMAEVAIESVANYAAADAETTLRLMPIM
                     QKELQRVNGTKLLDEIDMPLTPVLAEMEMTGALIDTKFFAAMSQELGQRLAEIEKEIF
                     QLAGKTFNLNSPQQLSDVLFNHLRLEPPDKGRKTTTGFYSTSADVLEALRGKHPALDF
                     ILEHRELSKLKSTYVDALPAAMDPNTGRVHTSYSQIGAVTGRLSSNNPNLQNIPIRTE
                     TGRRVRNGFIADKGNVLLSVDYSQIELRIVAHMAQDEAMLKAFRAGEDIHATTAAAIY
                     DIELDNVTKDMRRHAKAINFGLIYGMSAFGLTRSTDLTLAEAEDFVKAYFTKFPGVKK
                     YLDGMRRQAAEIGYVETLLGRKRYFPALQGKINMQLKNREEREAINAPIQGTAADIMK
                     LAMLKIPSALQAAGLKGKMLLQVHDELVLECPKKEVEKTAKVVQETMAEAYPMSIPLE
                     TEARAGASWGEMTVLK"
     gene            11653..13968
                     /locus_tag="PLE39_04305"
     CDS             11653..13968
                     /locus_tag="PLE39_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE67577.1"
                     /translation="MPGREDVFQKAMNDGHSAAWDQEWDKACAAYRRALEEFPDHPKA
                     LNSLGLALYQLGNFEEALQIYRNVVRLSPDDPIPMEKVSQISERIGDLKTAVEAAMRA
                     GDLFLQQRDTEKALENWVRVTNVNPENAIAHSRLAQVHERLGHMQQAVTEYLAIASVI
                     QRAGNAEKTQEMVSKALSLLPDSPEAKQAQTSLKMGQLLPKPMRGKGGTGPIRMAMVK
                     QLQKPTVSQSASGLDPIAEARQKALTQLAELLFEFSDDNPSAQEKRGLSAIMKGTGGL
                     SLQQSEQTKAVLHLGLAIDAQTKGNETQAAEEMEGALEAGFKHASLYFTLGLLRFKGN
                     RLESAQRFLQNSVKHNDYALGTRLLLGQLLVKKSLYDEAAIEYLEALKLADSITVPAE
                     KADEVSQQYEPLIEAHQSQKDENVLRKVCENINGLLMRPDWREQLHKTREQMPRQEGE
                     MLAPLADVILEAQSSSVLESMNRINQLARIGSLRSAMDEAYHAVQYAPTYLPLHALMG
                     DLLVQENRDADAIAKYSIVAHLYGVRGEVNQATKLLRRIIQLAPMDLGSRNRLIDQLV
                     ARGHADEAVREYLELAAIHYRLAELDLARKTYTTALRLVQQGNANRDWNAHIMQRMAD
                     IDLQRLDWKQALRIYEQLRSITPDDEATRRQLVDLNLRMAQPDKAMSELENYITYLES
                     QGKNDLSITFIQDLLQEHPDQLLLKRVLAARLHFAGRTEEAISILDVLGENLMQTGDK
                     HGAMEVINQIILMNPPNVSDYRQLLIQMQGN"
     gene            14129..15157
                     /locus_tag="PLE39_04310"
     CDS             14129..15157
                     /locus_tag="PLE39_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNE67578.1"
                     /translation="MLKTQNTAVMKWLYVFAFVVAFLVVFGGFVRLTRSGLSIVEWNP
                     ISGAMPPSGQQAWEEEFAKYQETPEYKLINTGMTLPEYKFIFYMEWFHRNVARLAGLF
                     YAFPVFYFLFKKTIPFKEFGIYFVMGILFISQAVAGWIMVASGLDKRPAVSHFNLTIH
                     LLLALTLFSLALWTAFGHKYGFHDTSKKAAWSLPSKLAVFSFVILAIQVSYGGFTAGL
                     KAGHVSDTWPLMLGKLIPPNLFNSWINIFESPQTIVFIHRWFAWLGLILVPAVYYLVK
                     KQNYPADIQNGLKWLIGVVALQITLGVLTILSYVNIVIALIHQANAIVLVGLAVFFIH
                     RFRAVDRK"
     gene            complement(15233..15427)
                     /locus_tag="PLE39_04315"
     CDS             complement(15233..15427)
                     /locus_tag="PLE39_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67579.1"
                     /translation="MRSPRFLLTLGLFLMILGIALPFMMVIHVIESTFLINFFSWASS
                     VVGLALGMIGATFMAARRRE"
     gene            complement(15424..16428)
                     /locus_tag="PLE39_04320"
     CDS             complement(15424..16428)
                     /locus_tag="PLE39_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE67580.1"
                     /translation="MTVNFEEPKETLLSIRDLHVWYELRRFGFGHAGYVKAVDGVSFD
                     LAEGETVAVVGESGCGKSSLMKTILGLNIPTSGEVIFGGVNLAGLKGNLLRQYRFEVG
                     YVQQDPYGALPPFMTIQQILEEPLIISGVKDKTERLDRIRKAMDEVKMYPANDFLPKF
                     PHMLSGGQQQRIVIARAMILGPKLIVADEPVSMLDASVRVEILKLMRTLQEQHQLSVI
                     YITHDLSTVKYFSSRIFVMYAGNLIEKADTRRLLDNPLHPYTMALLAATSDPDARNAD
                     TYKEVPPGEPPSLVNPPKGCRFHPRCARAIAGLCDQKEPVDFEPEPEHFVACWLYQPQ
                     "
     gene            complement(16480..17061)
                     /locus_tag="PLE39_04325"
     CDS             complement(16480..17061)
                     /locus_tag="PLE39_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE67581.1"
                     /translation="MSPQALYDVLYMFKDIHKVILDFASRLDDDQMHWRPRGYSTSIG
                     FHLWHLARESDYLKAIILERTPQLVPEFGTPLEIWVKEGLAQKWGFPTGLNATVGTGL
                     SDEAVETLPIPRKEELVDYLQRSYDAVEEFVERLDERYPNFDDVDDELKKKLQNIRLN
                     LLVFLTHDCRHLGMMECLKGLQTGFGSATENRK"
     gene            complement(17080..18042)
                     /locus_tag="PLE39_04330"
     CDS             complement(17080..18042)
                     /locus_tag="PLE39_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE67582.1"
                     /translation="MNAAPEKLLHIKNLVLHFKTANGSVQAVDGVDFDLDSNRAVVIL
                     GESGCGKTSLSKALLRLLPRNVDKYSGHVYLQGTDVMTLDNEEYRQNVRWVGMSLVPQ
                     AAMNSLNPVLRVGDQVAEPAMVHLGLSKVDAYKLVYKMFQHVGVPADFVERYPFELSG
                     GMRQRVALAMALVTSPSLIVLDEPTSALDLLTQANIMNVLKRIKHELGTSFILITHDI
                     ATSSELADEVAIMYAGQIVETGDAKDFFPAPLHPYSQMLMASVPRLRTDSDPMFITGQ
                     PPSLMNPPKGCRFAARCPYRFEKCSEEPPVFQKANRKVKCWLHA"
     gene            complement(18039..19376)
                     /locus_tag="PLE39_04335"
     CDS             complement(18039..19376)
                     /locus_tag="PLE39_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE67583.1"
                     /translation="MNNFRSSFQKLVQYPSAVAGIFVVFALVFVAAYAMITIPYTEAV
                     RLWRGGEEVWYQNPRFAAPAWINYFSSKQYSESFAVNTKDGQMTKTVTPGNEGISTFD
                     MAYEFDYSYDVYPQDLILYVTTTYKEKQPFISVELLTPDERTIRIGNFSVSNEFTYRF
                     SQDEKLLTKLRADEVIPALFSLPGSDQPLKGKYKLIITGTTFEPDSDLNVEFVSHGQV
                     FGWAGTDHARRDLVLPLLWGVPVALAFGLIASMGTSILTMVIAAIGAWYAGWVDELIQ
                     RITEVNLVLPFYALLIMIGTFYSRSIWVILGATIILSIFTGSIKAYRAIFIQVKESMY
                     IEAAKAYGASSPRIIFLYLIPRMIPLLIPSLVQSVPAFVFLEASLAVIGLGDPVLPTW
                     GKIIQDANSNGALYKGYYYWVLEPAMLLMITGLAFAFLGFALDRVFNPKLRDV"
     gene            complement(19382..20500)
                     /locus_tag="PLE39_04340"
     CDS             complement(19382..20500)
                     /locus_tag="PLE39_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE67584.1"
                     /translation="MSATAQPLENVVVKKSFLNSSFARILRYSAKRLLTLFITVVIGI
                     YLTILIANMGGFVDEIIKGQIRDSVTQKIANNPTALQMAPDVRKKLIEDQIALEEKRR
                     GLDTPIAVRNFRYLSNALQLDLGRAINLTSDSGSKQVRLILLERLPATLLLMGTSQLF
                     LFFSSIFIALSLSRKYGSFLDKLVIALSPTSAVPPWFYGIFLILIFAAALKVLPFGGM
                     VDSPPPDNTFDYSMSVLKHLTLPCLALILSSFFLSVYNYRTFFLIYSSEDYVDMAKAK
                     GLPTRDIERTYILRPTLPTIITNFSLLIITLWTGALFTETVFLWPGLGRTLFQAIGQY
                     DTPIIVGSSIIFAYMLAMTVFLLDFIYALVDPRVKIGG"
     gene            complement(20560..23148)
                     /locus_tag="PLE39_04345"
     CDS             complement(20560..23148)
                     /locus_tag="PLE39_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE67585.1"
                     /translation="MKLKKLYALFSLLVVFSMILAACAPAATEAVEEPAPTDAPAATE
                     PPTTAATEAPAAPATDRRGGWLDEIDFSVVAADSAISQLQAGTIDFYSFALASDAFPA
                     IKEAGLGYTQSFGGSYAISMNPAVFTDTAVLNPFSNRKIREAMNWLIDRNYINQEIYG
                     GGSLPKLFSITTQLVEYTNLVETARALETKYAYNLDQAKEVISAEMVGMGAELGADGK
                     WQYNGAPITLAFLIRSDGDGTRQPMGDYVSNQLEAIGFTVDRQYKTSSEAFPIWQGTD
                     AKDGQWNLYTAGYLPSGLTRDERANFQQYYLNTSVQAAEPFLSNVSDPEFQKLGDDLA
                     LGNFSTKEERDAMMAEALRLSLEDSLFVFVIDQQTYAPFNSNVSVTYDLAAGYESTFM
                     GNYNLRFKDQEGGTMKIGTNDLFTQPWNTIGGSNWVWDSNVMRATTQGADSITGTGGI
                     MGDPYTGLAWPQRIESASLTYQKGLPITHNLDWLSVEAVDQIDVPEDAWVDWDAANQK
                     FITAGEKFPDGTTAKVKSVVTFPADLYDTVKWHDGSSISVADFVMSIIIFFDRAKPES
                     YVYDEAAVPGLAGFLPSFKGYRIVSTDPLTIEAYSDSYNADAELNILTLWPNSPFGLS
                     GENSWQILAVSNLAEAAGELAYSSDKADVAQIEQMSWVGGPSLEILEKYMDQAQSENL
                     IPYEPTLGQYLTAEEITERYANMKKWYEDHGHFWVGTGPYYLDQVFTTEKSLVLKHNA
                     EYVDASDRWSRFSAPKLASTILDGPGQVTAGQEAVFDVTVNLGDAAYANADIKLVKYI
                     LYDTTGAVVSVGEAQAVGEGQFQVTLDAEATSKLQTGSAKLEVAVVPIPVAIPSFTSF
                     DFVVIK"
     gene            23528..27286
                     /locus_tag="PLE39_04350"
     CDS             23528..27286
                     /locus_tag="PLE39_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNE67586.1"
                     /translation="MRKYFVYAIFLLTLLGNSCVAAPDTLKPPLPVVFDEPQSGLNSS
                     RNSRSTPSYSQNIRFEHFSLEEGLSQSTITVIFQDSKGFLWIGTEDGLNRYDGYTFKI
                     YKPNPEDPNTLTDRWVTALAEDPEGNLWIGTRLGGLNRYDPETGQFSHLLYSNTNSAG
                     ISHNHITALSADKDGIWIGTKNGLDYLSYGSQRIEHFRASSVNPNSLSGNWISAIIQD
                     ANGNLWVGTQDSGLNFYDKTKKSFSVFKHSDDVFTSISNNHITSILIDNRGVLWVGTA
                     NGLNNSESAGRYFTHYVNVDGDSDSLSGDVVYAIYQDRSLGIWIGTDNGLNRYDPQRE
                     SFVHHNHQPTTPNSLANDTVISIFEDNSNVLWVGTDGGGLNKYNRQQDHFAYYRANPD
                     DPTSLSGNFIMPIVVGENGTVWVGAYGTGLNRLNAISNQFRRYVHDPNNSNSISTNDV
                     TALKIDSSGNLWIGTSIGLDRLNADTGQFTHFVPDINNPESLSAAPVNVIYEDHSNTI
                     WVGTSRGLDQFDPQTQVFIKYDTEDNDPNSFDNNQITSMLEDENLNFWIGTFDDGIKR
                     FNPSNGEITSYTNNPDNPSSLGSNAVLCLHIDRAGTLWIGTAGGGLNRYNPETDSFVR
                     FTEKDGLPNDVIYGILEDKNGFLWASTNFGITRFDPKTNTFRNFTASDGLQSNEFHQN
                     AFAIDLKGNLYFGGNNGFNVFTPDAIDSDTVAPKIVLTSLTRDRTPISNNIPSESIKE
                     ITLQWPQNSFEFEFAALAYEQPSKNQYAYILENFEANWNMIGTQRNGRYTNLPGGSYT
                     LHLLGANSDGVWNKEGVSIRVTVIPPIWETWWFRSSLLVFLAASVFLGYRWRVKRIEA
                     RNRELERLVQRRTGDLEKRTREIEALYQADERILRNVTLIQVFQTLVDVSVSMLKADR
                     SMVFIWNEEKNKITPRVSYGFRTETLYALHFDQGEGLVGQTMKTGETLIVMDIKRGTL
                     RGDVETVIRREGIQSFAHFPIVVDGNVIAIFNVAYTRPNAFTDDTIRLFTSLVNRAAL
                     SIANMQLFEQTKDLAVMEERNRLARDLHDSAKQKAFAALAQLGTANGILKTKPNDVKV
                     HLTEAETLVFEVIQELTFLIQEIYPIALQEKGLPTTLREYIFEWENRNDTEVKLTIRN
                     ERPLPLEIEQAVYRIIQEALANVARHSKAKRADVSLVYNSDSLQIMVADDGHGFDMNQ
                     RAKGMGFRSMRERISSVRGTIQIQSAPGQGTRLIAQLPIKSPGKEAST"
     gene            27283..27954
                     /locus_tag="PLE39_04355"
     CDS             27283..27954
                     /locus_tag="PLE39_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE67587.1"
                     /translation="MKHHRTSILIADDHEVVRNGIRSYLETLSDFQVVGEASSGEEAL
                     LMVAELIPDIVLLDLIMPGMDGIETTRRIKQTSPRTQVVVLTSYHEDVHIFPALKAGA
                     IAYILKDMKMEKLSEVLHRAVQGEVTLHPRVAARVLQNIRGENGEEQPLFTDLTDRET
                     DVLKLIANGLTNSQIAEKLVISENTVKGHVSNILSKLHLADRTKLAVYAWQQGIVNRD
                     SAPRE"
     gene            complement(28017..28787)
                     /locus_tag="PLE39_04360"
     CDS             complement(28017..28787)
                     /locus_tag="PLE39_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNE67588.1"
                     /translation="MNNPKELSKRQARREQIRRQSQRQRFIGIGLVVLGALAVAFLII
                     WPNLKPITGIISVEPNPRPQADANNMGDPNAPVKLVEYSDYQCPFCERFASETEAKLV
                     EAYVSTGKLYFTYRSAGNWVSDNIRGGKTESEDAAKAAYCAGDQNLYWEMHDMLFANV
                     LGEDVDSFTDRRLAAIAEQTGVDMTQFNECYSSGKFDDQVFQDGKDALTAGVRGTPGF
                     VLSYVNASGEEVTQLIEGAQPFDVFAQAIDAALAAAGQ"
     gene            complement(28877..29716)
                     /locus_tag="PLE39_04365"
     CDS             complement(28877..29716)
                     /locus_tag="PLE39_04365"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNE67589.1"
                     /translation="MKHFETSWKAHDGLEVFAQGWEPTVLQPKGVVCLVHGLGEHTSR
                     YAHVAEAFSREGLILFGPDLRGHGRSGGKRGHINSIEDFMTVIDQLLEQARNRYPNLP
                     IILYGHSLGGVLVLHYTLLRKPNIKGVIATSSGLRTALEDQKVKVMLAKVLGSVAPSV
                     TIPSGLDPKTISRDEKVIQAYINDPLVHDQMSFGFGKIMIGVTSWTLAHASEFPLPLL
                     LMHGKEDHLTFSAGSSEFAAPLKERCTLVLWDGAYHELHNEPEKAEVFKTMALWMDAR
                     LRE"
     gene            complement(29713..30324)
                     /locus_tag="PLE39_04370"
     CDS             complement(29713..30324)
                     /locus_tag="PLE39_04370"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67590.1"
                     /translation="MTEIGFPVHILKRFVETLSAEMGHETLTGALTNAGLPAEWAHSA
                     HFSALDDQRAANVYSRLQSALRKYYGRGARSTLLWIGVKLWEQLLNDSAFGIKAQAAI
                     IQRLPKGLRRKPALELLARILSATSGNVSVHSLDLDLLFVDHASPTAMEQTDTAPICF
                     VTLGLIRECLFWAVGQEHDIEERACRAMGARQCEFKISIGGEA"
     gene            complement(30332..31189)
                     /locus_tag="PLE39_04375"
     CDS             complement(30332..31189)
                     /locus_tag="PLE39_04375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE67591.1"
                     /translation="MININLQARRMVAEDYNQVSSLVYYEANNHRHLDWHSPLERINL
                     PNCWVLAEGASIHAVLICPEDPPNVAWVSFFGYSPSLSGAEAWSLLWEKARSDLASEN
                     RPLKVAAIVVRQWFQPLLLSSGFINQQNIVLLELRNEDIRYFLTPHGVRIRPMTDADI
                     PMVAKLDLAAFGDFWHNTASTLQRARLQCASATVAEDDSGVIGYQLSTRNPLGAHLAR
                     LGVSPEAQGRGVGAALVSDLIQSFGGAQLHRLSVNTQSDNEASLALYKKMGFVRTGEY
                     FPVLVYPLG"
     gene            complement(31186..31707)
                     /locus_tag="PLE39_04380"
     CDS             complement(31186..31707)
                     /locus_tag="PLE39_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67592.1"
                     /translation="MTIEPAQLAEQGKQAFRNKQFDESAELFRQAAEGFSLGRNGLLA
                     AEMKNNMSVALLQAGKAQESLDAALGTDLVFAGAKDIKSQGMALGNQAAALEALNRFD
                     EAIAAYERSAELFAEVGEGDLRAMVMKSAAAIKLKTGKVTESAFKMMGSLDAKDNPGF
                     FERILKFFLRFIK"
     gene            complement(31762..32436)
                     /locus_tag="PLE39_04385"
     CDS             complement(31762..32436)
                     /locus_tag="PLE39_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE67593.1"
                     /translation="MNNAQIIIREKKLGLLIRDARMAERRSIKECADAIGVKPGLFRA
                     YEEGRRSPSLPELETLVYYLKLPITHFWGRETMSETSSPAETLDTAQLIALRQRMIGA
                     LLRQERNKINMSLRQLAVDTGIKSARLNLYELGERPISVPELESILAVLGSRIETFFD
                     QNGPVGQWMTSQRAMQKFLELPEEIQNFVCQPVNRPYLELAMKLSDMSKEKLRSVAEG
                     LLDITL"
     gene            complement(32566..33879)
                     /locus_tag="PLE39_04390"
     CDS             complement(32566..33879)
                     /locus_tag="PLE39_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNE67594.1"
                     /translation="MTTVTPSSRLTGQDLSRLKKYLADNLMSAMGSERVPAAERSAFV
                     KKNIVHVYEQTQLKLPEDLRKQIFEQVLNDVLGYGPIQSLLDDEDVSEIMVNGPKKVF
                     VEKKGQLTKSTVTFDDDDHVMRIIDRIILPLGRHVDADSPTVDARMPDGSRVNAVVRP
                     VAIDGPSITIRKFRKDKLKPEDLINFGSLTRQMADFLQACVKARFNIIISGGTGSGKT
                     TLLNVLSGYIPENERIITIEDAAELQLQQDHVMRMETKVANAEGLHAVTIRELVKNSL
                     RMRPDRIVVGEVRGGEALDMLQAMNTGHDGSLTTVHANTPRDAISRIETLVLMAGMDL
                     PIKVVRQQISSAVDLIVQQTRLKDGQRKVTAVTEVAGMEGEIIVLTDVFKFIQTGVTT
                     EGKVLGEISATGIRPNFTPRLEAAGFKLTADIFAPKASANQGSRR"
     gene            complement(33882..34172)
                     /locus_tag="PLE39_04395"
     CDS             complement(33882..34172)
                     /locus_tag="PLE39_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67595.1"
                     /translation="MTIEYDEKGKFYTEVVTKVPTAVVIQTTTHLIRGLVHVRQGERL
                     KDELEMDERFIAVTNFTIQDANEKVLFSGAFLAVLRAQIIWITPADESSQGG"
     gene            complement(34300..35454)
                     /locus_tag="PLE39_04400"
     CDS             complement(34300..35454)
                     /locus_tag="PLE39_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE67596.1"
                     /translation="MSEKKILIVDADVASRNFISRNLQAQKYEIIQTGSGKEGLIHAW
                     RDRPDLMVIDPTVPDITGEEVARKLKQDARTTNMPLIALSSDLGVARIKSCLDAGFSE
                     YIAKSGQAMATLTDAINRLLGISSALVKEGGLLMAFISAKGGVGTSSLCANLGLTVCQ
                     NQPEARVAVVDLVLPIGSIGSIVGYEGSLNIVTASDMPEEKITPDYFRDQLKEAGIWR
                     FHLLSGSPDPESSNHLKVGRIWNVIAALKASYDYVLIDIGRALSKISLPILQHADLVA
                     LIISTDVSALPLTKTVLDYMKIKGVEPATIFPILNRASGLEGLSKPDAEKALDIQIKA
                     AMPYLGSSFAFASSHHQPFSLKFPNDTASFVFLETAKEMAVLAQKLRTGQ"
     gene            complement(35498..36193)
                     /locus_tag="PLE39_04405"
     CDS             complement(35498..36193)
                     /locus_tag="PLE39_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE67597.1"
                     /translation="MSKRILIVDDEPRYLRLLEANLRTEGYEVATAQDGVQALDVFSV
                     QPIDLVLLDVMMPRLDGFGVCQRLREFSNVPIVILTARGEEQDRVRGLDLGADDYLVK
                     PFSATELLARVRAVLRRAQAPTESGQARFFTHDDLKIDFARAEVWRGEEPVSLSATEY
                     RLLLQFTHNVGKILTSEDLLTSVWGVEYKNDKEILWVSIARLRQKLEADAHSPRHIVT
                     RSGLGYLMPPTEI"
     gene            complement(36227..37414)
                     /locus_tag="PLE39_04410"
     CDS             complement(36227..37414)
                     /locus_tag="PLE39_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE67598.1"
                     /translation="MDLATLTFLHKTFQEVQIQQDWRAALDALFVSMRGSFVFDNAAI
                     YLEDPRTKGLEVVYARAVGRGKAAEADSMWGEGIANEVLSKGRMILKEPRKNLNYANR
                     LTEAHLLGFPMYVGGKLNGALVFIRFGGPEYSNLHIQIASLQSFWATALIERRDLQEA
                     RTELDSVQRQMRLQDDFVSTISHELRTPLGFIKGYSTSLLRQDTTWDETTQREFLNII
                     DEEADRLARLIENMLESARLQSKTLQFKFSPIRIDALARDVAMRIHTHHPELKVNFET
                     DSLPSIRGDGMRIAQVFENLFSNALKYAPNSEITIAMRMQGDKIRVSFADKGEGIPED
                     YLPFLFERFYRVPGERTVTGTGLGLYICKQIVMAHHGNIWVESELDKGTTFIIELPVD
                     STL"
     gene            complement(37417..>38454)
                     /locus_tag="PLE39_04415"
     CDS             complement(37417..>38454)
                     /locus_tag="PLE39_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE67599.1"
                     /translation="LPRNPMSQNLPKLTPEMLIPRMGEYIVQKGLITEADLQRALTYQ
                     QDQIAKGNHYLLGQSLMDLNLLDRDTLDQVVTEQILQLRSALQSANRNLEQRVKERTA
                     ELNEALQRLSELSQLKSNFVSNISHELRTPLTHVKGYIELLVAGSLGEISEEQRHALQ
                     VSQRATAKLEGMIEDLIMFSLASKGEMSMKLQRVDIRRIVSLAAKSALPKAEERGVQV
                     LVSALENIPSVQADPEKIGWVVSQLLDNGIKFTPSGGSVVISLKEEGHNLVVLTVTDT
                     GIGIPSNKIKEIFEPFHQLDGSSTRHFGGTGLGLSLVRQIVEAHGSLLDVQSAEGKGS
                     VFKFPLLALGD"
     assembly_gap    38455..38554
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(38556..40145)
                     /locus_tag="PLE39_04420"
     CDS             complement(38556..40145)
                     /locus_tag="PLE39_04420"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNE67600.1"
                     /translation="MKLLYNANIHTLDSSHPQASAILIEGGRIVAVGEKDTLEAIAHG
                     RVEKQDMGGRTILPGLTDAHLHLQHYGLSLQKVDCETKTKAECLRRVAERAQKAKPGA
                     WILGHGWNQNEWTDDNAFPAASELDAIAPNNPVYLTAKSLHMGWANTAALQRASITND
                     TPDPKNGAIQRDNTGRATGILLETAMSLVESTIPEPTISEIESAIENAQSILWKMGLT
                     GVHDFDRRDSFMALQHLHAQKKLKLRVNKNIPVESVDHANALGLRTGFGDDWLWIGSV
                     KAFMDGALGPHTAAMFQPYQDEPENRGILNMDGEELFEHCRKAADVGLSMTVHAIGDR
                     ANHEVLNAYQQLRAYETEKGLPHLRHRIEHVQVIHPDDAPRFAQLNVIASMQPIHAPS
                     DMFAADRFWGDRSAHAYAWRTQLNHGATLAFGSDAPVESPNPFLGLHAAVTRRRADGS
                     PSAEGWYPEQKLTLAEALSAYTVGAAYAANAENRLGKLAQGYLADLIVLEENIFSADP
                     QSLLTIKAKSTMINGEWVLED"
     gene            complement(40388..40903)
                     /locus_tag="PLE39_04425"
     CDS             complement(40388..40903)
                     /locus_tag="PLE39_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67601.1"
                     /translation="MKKTRNTKGTLETLLVIGLILATLTACESKPLEVTDISVHPDPV
                     IGQTATLRVEIMSINDEKDVTINITLPDGVKLMSGDLEWKGSLIANQPQAHELELCIL
                     YEGEWGFFISTYSQYAPTSRYGDAEMLYIITSADTVRVVSSGKYHVTQPPGGMILPTP
                     IPQTPPTGICS"
     gene            complement(40983..41516)
                     /locus_tag="PLE39_04430"
     CDS             complement(40983..41516)
                     /locus_tag="PLE39_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67602.1"
                     /translation="MKKINTQKRMASLFLFIGLTVSACSSSGGYPLNITSVKVFPEPI
                     VGEVVTLEVEIVSVDDKANVKFTIDTLEDTGNKIHLVSGEPAWQGTLTANQPKIFQVK
                     ICVMEEGSWPVRLYTALPSSDQEGGYDFDIIHLESTLDSGKLIPSSEYTFSQEEYANR
                     PTPRPITVSSECSGISK"
BASE COUNT        10621 a        11422 c        10088 g         9635 t
ORIGIN      
        1 gttatttcag ggcgtacaca gcgtatccac gcgggggagc gaagaattgt ccgcggccat
       61 cggcgccagt ggattggttt acggggcggg acaggtcatt tttgctccac caggctacgg
      121 gttgaaaaga ggcgttcgtc cattgcgtat tgacccattc tccctgccag cgttcaccgt
      181 gattattgag cacatagatc aatccaggct gattttcata cccgccgcgc tgcatgatgt
      241 agagatcatc tgaaagatac agcgtgctga catttccgcc tgcgtatttt tcgtgcgcgc
      301 tgatgagcgc atcgatgcca ttgggcgtat cacgcaaggc aaggttgtaa ttgaaataat
      361 ccttccaaaa tacgcaggga taaccttcat gcgtgaagat gtaggaataa gccagcagtt
      421 tatcgttggt gatgggtcgg ccttcgtcgc gcagatcgtg gttttcaaca aaggtcaccg
      481 tgctttgcgg ctgggctctc aaaatggatt caccgttgac gaggctcttc aaactgaatc
      541 catacgaatc gcacagccct ttgagcaatt cgcgcaacgg aaaatcaaac gcatccacag
      601 ggttcgagtt ggagaagttt gccgcattga cccagttgag cgtgttcgag gcattgtccc
      661 aatattccgc cacgccgtac ggcctgtgat acgcgccgtt cttcatataa cggtattcct
      721 gaatggctgt gatggtgttt gcgccatagc ctttgacaaa gtcgtagcgg aatccatcaa
      781 agccaacctc ttcgatgagc cagcgtgcga gcttgagcaa ttcggcatac acatacgggt
      841 tgcgatgcga aaggtcgggc atatcgccga aggtcatttc atcccagctt tcgtacatgc
      901 tggggtgaaa cgattcccaa ttgcgcggga aacgctcgct catgggatta aacaccgtcc
      961 aacgcgattg ccccgtgatg ggattcactt cctgcaaatc cgctccactg ttgtgattga
     1021 ggaccatatc cgcgatcaca cttaacccgt gcctgtgtgc gctttcaatg agttgactca
     1081 gctccgaacg cgttccaaac caggtaggga cgctcccctt ctggtcgaat tcgccgaggt
     1141 cgtaaaaatc gtatgggtcg tatcccatcg aaggtccacc caaattggcc gccttgtgga
     1201 caggcggaag ccacaacgaa gtaaaacccg ccttcgcaag gttgggaatc tcacggttga
     1261 tatagttcca ccactggaat tcttttttat cctctctggg acaatcccag tagaaggttt
     1321 gcatcatcac acccattcgt attgctcctt tatcttttat ccgcacacga aagcggattt
     1381 caagcaggta ttttaatggg gtttacagaa ggaggaggtg gacgaaagtc ctaatcttcg
     1441 atctcacaac tcttcattca ggtgcaaaca cttcagcaca accgttactt tttgagttct
     1501 tttgtactca gcaaataaag aataccataa cctcccaaaa acactccagc caagatcgca
     1561 atgtatttca agtatcctgt atcggaataa cctatatcca tcagccaatc tggcatagga
     1621 cgcggcaatt cgctggcggt gctgtcgatc cagtacagat cctcacttga aacccatact
     1681 tttccgtcgg catcaagcac aaaagaagag cggccagatc tggggtagtt agaattttct
     1741 ttgttgtact gcacaacaag ttctgcaaca ccgctccaaa caggccgcag tactgtaact
     1801 ccgcctccat atgaatcatc caaaatccat aacctgtcat gcgaatcaac cagcaaggaa
     1861 tcataatcag aaaattcccc taaaagcgga atttcagtga tcaaagcctt ttcatcgtct
     1921 tgaatttgaa caaaataact ttcaacttct tccgtctgcg tagtatagaa aataaagaga
     1981 cgccccacat cgtccacgat gaagtctttt acctgaagaa aatcgaaggg cacatcataa
     2041 gacttctcca gccattgtcc atctttcaac agaaatattc tggcttcatt ttcattccca
     2101 gcatgaacca aaactgtctc atccgccaaa atggaaaggg aatagggctc atatcctttt
     2161 tgaactgcag attctgggag ttggatcaca tcaacaccag gcctgccagc tattgcacca
     2221 accaaaacat tgggtttgtt atctccgtac aaaaccagcc aggcttgttc cgtcgtactc
     2281 acaaatctct tacgatagat ggcagcctta taaaccccga aatatttcca ttgcaaccat
     2341 ttgccatcct gccaggtgta attgatttca ccagttatca gagttggctg gccattagaa
     2401 tcggacagaa taatcgttcc tcgagggttt tgcagcggaa gcggccatct ctgctcctga
     2461 tcatcaaaat gaacaatcag attgtcacca ttaaaatcag tagaagcaat ccaaggtgag
     2521 ttcttagaag tgattgaaaa gtcgagcaaa aactcgttcc agggaagtct atgaacattc
     2581 catccgccct gaaaaaaacc gtatacacca agaactggaa tcgttatgag ggggagaaac
     2641 aataccatcc ccgcatcgtt aatctttaca aggatggaat ttttttccat cttttctggc
     2701 aatttttcat ctatcactgt tcctggcagg tgggttttta ggagttccat cactctatct
     2761 gccccacctt cgatatctgc caaaacaaat ttaaaatcct cttcattttt agagcgtaca
     2821 acaatgaccc ctggaaataa atactgcact gataatacgt cactgtacag aatccgccgc
     2881 gggcgggatg agtaaaaacc cttattttgg ttttcctcaa tcgcctcgct tgtaaacaca
     2941 atacgatatt taagttcttt ttttatacgg acaaaataaa accatataaa aaaaacaagc
     3001 actccaacag taaaaagatt aacgaaactc aactccagca gggatttcaa cgatgcccgt
     3061 ggcggatcaa acaaaaaagt taacggaaga attccaatca atccagacca aagcggaaga
     3121 attagcggac gacgatttaa ctttagcgta ttcattttcc tccttacact caatgcaaca
     3181 tagttttcaa attaatttta ccttctgtcc cgctaaacct ccaccctcac caccaacgcc
     3241 gtcctcgccc actcactcgg acttctgcct tcccactcgt tgtacttgat cgccatcttc
     3301 atccgcacca acgtctcggt ctgtccatct ttcagatcca gcagcttcgc caccgcaggg
     3361 aaggtctgtc ccgcgattcg cactgtcaca ttcgggtctt tgagcaaatt cctcacccaa
     3421 tcagacctgt ccatattgcc cgataaaaga taaagcgccc cttcatgcac acagaaccaa
     3481 atctcgatct catgcgggtc ccctgttttg cgtccgcggg tggtgaggta gcaatattgc
     3541 tctttttcaa attgcgtcag gtcaacggcc atggtcaaga ctccttctcg ttcgaaagga
     3601 aatacgtctg tgcagggagc aggatatgca cccgtttttc agtggcatac accgtggtac
     3661 agcgtccgcg cggcaggcgt ttaatgagcg aagaatcgta caacaaaatg gaacggctcg
     3721 tgatcttctc gatcaccgtc caatgatcgt gataaccctc caaccccaaa atgatggaac
     3781 gtcccggcgt gccatccaga aatgcctgca tcgaatgcca aaacgtggtc aggtcggggt
     3841 tcggcacccc gcgccacggg atctgcacat tcgaaatccg cttcttgccc accaccttgt
     3901 ttaagatcaa cagcatctgg tgatgcaaga tccccccgat cagaaactta cccagcaaac
     3961 cgcgccgcga aagataatgg atcaagtcat cgaacaactg ctgcgtctct tcatcggaag
     4021 aatgattgat aaaccgctcc gcattgacaa tgctgtacaa cccgcacaac gaatccaacc
     4081 caccctgttg aaaaggcgga aaccccgaac tgatgcgtga agaattcgtc ataaacaata
     4141 accctaaatc agatcactca accaaccgcc acaacaaaat acccgccgcc aaagccaatg
     4201 ccgccccaaa gaaaaaagga gccgaagcgc cgaatccagc ccatgaaccc agaccctgcc
     4261 acagcagtcc cgcaagaagc gaggcaggca gagccgtgat cccgatcgcc gcattaaacc
     4321 atccatacgc cgccccgcgc tgcgcctctg gcaccagatc cgcaacaaaa gcctttgcca
     4381 caccctctgt cgccgcgtaa taaattccat acaaaccaaa cagggcccag atctgccagc
     4441 ccgcacccgc aaacgcaaac cccaaataca ccaacccata cgccatccac ccgccgatga
     4501 tcaaccgcct gcgccccact ttatctgaaa gcgcccccaa cggccccgaa agaatcgtat
     4561 aaatcgccgt gaaggtcatc aacatgaaca acgtctgcag cacattcaac ccgcgttcct
     4621 gtgcgcgcaa aataatgaag gaatcggatg aattccccag cgtgaaaaga atcgtgatca
     4681 acaggaattt tccaaaccgt ggatcgatct ccttaaaaga cagacgcttc ttctccgcgc
     4741 ctttgcctga tgcagcaaca tcggcagccc ccaccccgag aacgatcacc gccaacaccg
     4801 cagggatgat gctcgccaac accgccgttt gaaacgcttc gcgtgtgatc tgcaatgcac
     4861 cctgctgggt cagccacacg atcaaagcag caatggcgat ccccaaaaaa gcccccgccg
     4921 tatcgccagc ccggtgcaaa ccaaaagcca atccgcggtt ggtttcatcg gtactggcgg
     4981 ccaccaacgc atcacgcggt gccgtacgga tccccttccc tacacggtct gaaaaccgca
     5041 ccccgagcac ccatccccac gaattcgcaa aatacaaaaa gggcttggca aaagtggaga
     5101 tcgcatatcc cagcaccgcc aatcccttgc gctgtcccag cttatctgaa agcgcccccg
     5161 aatacacctt caacaaactc gccgttgttt ccgcaattcc atcgatcagc ccaatgaacg
     5221 ccatccgcac ccccaacacg ttcgaaagaa aaagcggaat caaatacaca agcatctccg
     5281 aagaaatgtc tgtgagaaaa gaggtcacgg tcacgatcca aatatttgga tgtaattttc
     5341 gaagtgtact ccgcggtttc attttttcct ttttattgtt cgttcatcat ccatcactcc
     5401 cccatcaaaa cctcaagcat ccatcattgc catatcaatc cttcttgcca aaatacccaa
     5461 acccgatcaa caccgcagac atcagcgccg tggcgacccc ggtccgcaac aggtcattcg
     5521 tatccttcac ttccaacagc ttctgcagga acttcacccc catcaccaaa acgatcatac
     5581 tgctgagttt tgccttcaat tcatacaggt tgtgggccag catccactcc ggcagatcca
     5641 gcggagcaat gaaaagttcg tacaggctta cggcaaagat caaaatggca gtggcgatca
     5701 aaaaactgtc cacgatctca atgaactcga tggtgatcgc cgcatccttg cccattgact
     5761 caaacacaag cagcaccgcc gcggcagttt tcagggtccc ccacgcaaaa gccgccaccg
     5821 ccgcgatcaa cagtgaaagc acacccacaa ttgcaagata tttggatttc tcaagtaagg
     5881 ttttcatggt gtccgtttta ttcgccgtac atctgacgat acaaagcgcg caggtttcgt
     5941 cggtgcaaag tctctgccag gccgccaagg tacacgcggc tcttcccaca ttcttcgatg
     6001 gtgatcgcgt ccagatattc atctgcgttc ttgcgcttgc ccgcagcctt caccagtttt
     6061 ttgatattgt tcaggtagtt catattctct ttgaccgccg catcgatttc gccgcgcaaa
     6121 atgatatcgc catgaccctg aatgatattc tccagtccca tacgcccgat ccgcttgacc
     6181 gaggagatca tctcgtcaat atctccatcc acgatgtaag gcagggacat gaaagcatcc
     6241 ccggcaaaaa gcacgcgatc ctcttccacc aaaaccgaaa tgccgtcttc gctatgccca
     6301 aaagactgcg aaaacaccag attcttcttg cccacccgca tggtcaattc ccccgaatcg
     6361 aaggttaaat gcggcggaat gatcttcacc tgccgcaaat tcgggttttg tttacgcgca
     6421 ccctccaaag aaggaattcc atgctcgatc agcagttcac gacatttcgc atgcccgatc
     6481 acagtggcac cggggaagaa acaattgccc caggtatggt cggcatggta atgtgtattg
     6541 atcacataac gcacttgaac gccaagttca tgttcaataa actcgcgcat accaagtgtc
     6601 tcttcaggca gggccagcgt gtcaatcaca acggcccact gcgggccaac aataaccccg
     6661 gcagttacct gagcataaac ttcgctttga aaccagaaca cattttccga aactcgttca
     6721 cgatgcataa atcacccttg tatggatata ttttcattat taatagcata ctcccgccca
     6781 aattacaagt tgattagaca aattgaaaga ttttcattaa tgtaaaccgc cctaaaaaat
     6841 ttgacttgat tagccctctt ataagtgata aactctactg caaatgcctg cggatattct
     6901 tgtagtcacc tcttcttcac aacttggaga gtccatccgt cgcatcctgg aagagacgga
     6961 tctctaccgt atccatgttg tcaccaataa atcctctgcc atcgttcgcg cagatgaagt
     7021 tggtgcgcct cttgccatgc ttgatcttgc ccttggagaa ggtgtgattc gagagatcgg
     7081 acaatctctc cgcaccattc gtcccggcat caacctcatc atcctttgcg atgaaaacat
     7141 cacgccccct ccgtttgacg aattgcgccc ttggattctt gttcgtaaac ctttcaagat
     7201 gtcaaatttt atgaccgcga tcagtcaacc ccaaaccaca acagcaacac cccccacccc
     7261 cgctactaca acgtcaaccc cggcagccgc gccgcaaaat acctccacgc ccactctgcc
     7321 ctggctcagt gacccgaacc gggcggcgca gcaccttacc cgcctcacgc ttgagtcttc
     7381 tgcacaggcc gcattgatca ctcgtaaaag tgacctgtgg gcctatgcgg gcagtctttc
     7441 ccagggtgca gcaaaggaag tggcacaaac catcacccgc aattgggatg ggcaaaaagg
     7501 atttgacctg ttgcgcttca tccgcctcga aagcaccaaa gcggaacaca tgctttacgc
     7561 cacccgcctc gctgccgaca ccgtgcttgc tcttgttttc gacgcagaaa ctccctttag
     7621 caccatccgc gcacaggcca gtcaacttgt caccaacctc gataacgaaa ccgcggaacc
     7681 gcagaaacca tctcagccgt tcaatgcgga aaaacaagtt gttgaagcag atgccgatga
     7741 cggtgatgcg ccaaacatcc cgcttatttc agacatcctc aacaacgtac cgacacccaa
     7801 ccccgaaccc gccacaaccc gcgatttcaa cctgccccgt aaaaaagaaa cacaggaagc
     7861 cttcgacccg aatcagaccc gaatttcaga atcgctttcg aatgcatccg ttttcaaacg
     7921 agacccgtct cctgcagtcc attacggcag cataccggtc ctgaataaaa agccagctcc
     7981 ctccattcca gaagttcagg ttcagcctca aaaccaggct gtgttgaacg aagttgacgt
     8041 tacagcccca tcacgcattc aacaaaggcc tgaaaccccg gtggccaaac ccgctcccgg
     8101 cgaacaggaa gtcacccgcg aaagccccac caccgaggcc gcacggaaac tcgtcgtaga
     8161 accgacaacg gcaggcctgt accatttgac ctacgcctgt ctgcttgtac cgcgtttttc
     8221 agctcactac cttaccggtg atctggctga ccagttggga gaatggctgc ccaatatttg
     8281 catcgccttc ggctggcgac tggaattcct ctcggtacgc cccgaatatt tgcaatgggt
     8341 cgtcaacgta cagccgaata cctctccagg ttatctcatg cgcatcatgc gccagcaaac
     8401 ctctgaaaag atattcaccg atttcacccg tttgaagagg gaaaatccct ccggtgattt
     8461 ctgggcgcct ggctatctca tcatgggcgg aacccagccc cacccgcagc aattggtgcg
     8521 tgattacatc cgccagaccc gccaaagaca gggacaggaa ctcccatcgg aaccgaaacg
     8581 caagtaagtt ttccgacaca aacgaagcgc agagtataat cactctgcgc ttttattttt
     8641 cagctgtttc aaaaaatgga cctcttgcat aagcctaaaa tcttaaagag aaaaacgatg
     8701 aaggctctgc ctaaaagttt tttcagttac ccgcttatgt aagaagtcaa acaaaaagag
     8761 aatcccacat gccccctacc ctttatctca ttgatggaca cgccctcgcc tatcgcatgt
     8821 actttgcact tacagcaggc ggaggcagtc aacgctggca aaactccaaa ggcgagccca
     8881 cggctggcat ttacggcttt gcgcgtgaac tggtgcgcgt gctcgaacag gaaaaaccag
     8941 aataccttgc tgttgcattt gatgtaggca agaccttccg cgacgatatt ttccccgaat
     9001 acaagggcac ccgtgaaaaa atgcccgatg accttcgtcc gcagatcgag cgcatccgcc
     9061 agatggtgga tatgttcaat attccgcgcc tcgaaatgga cggctacgaa gccgacgatg
     9121 ttctcggcag cgccgcccgc attgccgcca aaaaaggact cggggtgaag atcatcacag
     9181 gtgacagaga cctgcttcag ttggtcaacg agcgcacggc tgtctatctt gctggtgacg
     9241 accagaccta catcaccgat gcagatgtga tcaaaaaatt aggcgtgcgc ccgactcagg
     9301 tggtggatta caaagccatc gtcggcgata cttccgataa tattcccggg gtgaagggtg
     9361 tgggtgaaaa gaccgccatt gccctgctcg aaaaattcgg cacgcttgat gccatctacg
     9421 ccaacctcga tcaggttgaa aatcgctgga agggcaagtt cgaagcaagc aaagatgcgg
     9481 cctacatgag ctacaaactt gcaaagatcg aggtggacct gaagatcgat ctcaaccttg
     9541 accatgccgc ggtcaggccg tttgattcgt ctgcattggc ggctttcttt caggaaatgg
     9601 aattcaagac cctgctcaac aaactgcccg ccatcagtgg aagcgcaccc tcagtggaga
     9661 tcgctgctgc gccgaaaaaa ggcaaggcag gcgagcagat gtccatgttt gtaaatgcac
     9721 ctgtcatcgt ttcaacacca tcgaagatcg aagtgacggt tgtggacaca gacgaaaaac
     9781 tgggcgcact aaaaaacgaa ctcacccggg cgaaggtcat ctcttttgac acagaaacaa
     9841 cttcaaccga ggaagtgacc gctgaactcg tcggcatttc gctggcggtc aaagagggtc
     9901 atgggtatta cattcccgtt ggtcatgttt ctggaaacaa ccttgaaatc aaaaaagtga
     9961 tcgctgcgct cacgcccgcc atgaccgatt cgaaaatcgg caaagtggcg cacaatgcaa
    10021 aatacgatta catcatgctg gcgcggtatg ggttggtcat ctcgccgctc accttcgata
    10081 cgatgctggc tgaattcatt gtggatccat catcgcgcaa cctcggtttg aagaatttat
    10141 cagaataccg ccttggtgaa gagatgactc acatcgaaga actgatcggc aaaggcaaaa
    10201 agcagatcag catggcagaa gtggcaattg aaagcgtcgc caactatgcc gccgccgatg
    10261 cagagaccac cctgcgcctg atgcccatca tgcaaaagga attacagcgt gtgaacggca
    10321 caaaactgct cgatgaaatt gacatgccgc tcacccctgt cctcgcggaa atggaaatga
    10381 cgggcgcgct gatcgacacg aaatttttcg cggcgatgtc gcaggaactg ggtcagcgtt
    10441 tggcagagat cgaaaaggag atcttccagc tggcaggcaa gaccttcaac ttgaattcgc
    10501 cgcagcaatt atcggatgtg ctcttcaacc atttgcgcct cgaaccaccc gacaaaggcc
    10561 gcaaaacaac aacaggcttt tactccactt cggcagatgt gctcgaagcc ttgcgcggaa
    10621 agcaccccgc actcgatttc attcttgagc accgtgaact ctccaaactc aaatccacct
    10681 atgtggatgc cctgcccgcc gcaatggacc cgaataccgg ccgcgtgcat acctcataca
    10741 gccagatcgg cgcggtgacg gggcgccttt cttcgaacaa ccccaacctg caaaacatcc
    10801 ccatccgcac cgaaacaggg cggcgcgtcc gcaacggctt cattgcagac aagggcaatg
    10861 tgctgctttc agtggattat tcgcaaattg aattgcgcat cgtggcgcac atggcacagg
    10921 atgaagccat gctcaaagcc ttccgcgctg gtgaggatat tcacgccacc accgccgccg
    10981 ccatctacga catcgagttg gacaatgtga ccaaagatat gcgccgccat gccaaggcca
    11041 tcaacttcgg tctcatctac ggcatgtcgg catttggcct cacccgctcc accgacctga
    11101 ccctggccga agccgaagat ttcgtcaaag cctatttcac aaaattcccc ggtgtgaaaa
    11161 aatatctcga tggcatgcgc cgtcaggccg ccgagatcgg atacgtggaa accctgctcg
    11221 gacgcaaacg ctacttcccc gctttgcagg gcaagatcaa catgcagttg aagaaccgtg
    11281 aagaacgcga agccatcaat gcccccattc aaggcactgc ggcagacatc atgaagctcg
    11341 ccatgctgaa gatcccctcc gcgttgcagg cggcgggttt gaaaggcaaa atgcttttgc
    11401 aggtacacga tgaactcgtg ctcgaatgcc ccaaaaagga agtggaaaag accgcgaagg
    11461 tcgtgcagga aaccatggcc gaggcctacc cgatgagcat tcccctcgaa acagaagcaa
    11521 gagcgggcgc gagttggggc gaaatgaccg tactaaagta gcccgccccg ttgcttcatc
    11581 cagtctggag agacggttat aattgcgtga taatttttca ttcagcgcac atgcgaaagt
    11641 agaataaaca ctatgcccgg acgagaagat gtcttccaaa aagcaatgaa cgacggccat
    11701 tcagcggcct gggatcaaga gtgggataaa gcctgcgctg cctatcgccg cgctttggag
    11761 gaatttcccg accatccaaa ggcgttgaac agtctggggc ttgcgcttta ccagctcggc
    11821 aattttgaag aagccttgca gatctacagg aatgtggtgc ggctctcccc cgatgacccc
    11881 atccccatgg aaaaggtttc gcaaatctca gaacgcatcg gcgacctgaa aaccgccgtg
    11941 gaagccgcca tgcgcgccgg tgacctcttc ctgcaacagc gcgatactga aaaggcactc
    12001 gaaaactggg tgcgtgtcac aaacgtcaac ccggaaaatg ccatcgccca ttcacgcctt
    12061 gcacaagtcc atgaaagact cgggcacatg caacaggccg tcaccgaata cctcgccatc
    12121 gccagcgtta tccaacgtgc aggcaatgcc gagaaaacac aagagatggt cagcaaggcc
    12181 ctctcccttt tgccagacag ccccgaagcc aaacaggcac agacctcatt gaagatggga
    12241 caattgctcc ccaaacccat gcgcggaaaa ggcggaacgg gccccattcg catggcgatg
    12301 gtcaagcagc tgcaaaagcc aaccgtatcg caaagcgcct ccgggctgga cccgatcgct
    12361 gaggcgcgtc aaaaagcatt gactcaactg gcagagcttc tcttcgaatt ctccgacgac
    12421 aacccttccg cacaggaaaa acgcggactg tccgccatca tgaaaggcac gggcggcctc
    12481 tccctgcaac aatccgaaca aacaaaagcg gttctacacc tcggccttgc aattgatgcc
    12541 caaaccaaag gcaatgaaac ccaggccgcc gaagaaatgg aaggcgcgct tgaagcaggc
    12601 ttcaaacatg cctcgcttta cttcacactt ggtctgttgc gtttcaaagg caaccgcctc
    12661 gaaagtgccc aacgcttttt gcaaaactcc gtcaaacaca acgattacgc cctgggcact
    12721 cgtctgctgt tgggacaact gctcgttaaa aaatcgctct acgatgaagc cgccattgaa
    12781 taccttgaag cgttgaaact cgccgactcg atcaccgtcc ccgccgaaaa agccgacgag
    12841 gtcagccagc aatacgagcc gctgatcgaa gcgcatcaaa gccagaaaga tgagaatgtt
    12901 ctgcgcaaag tctgcgaaaa catcaatggc ctgctgatgc ggcccgactg gcgcgaacaa
    12961 cttcacaaaa cccgcgaaca aatgccgagg caggaaggcg aaatgcttgc cccgctggcc
    13021 gatgtgattc tcgaagcgca atccagctcc gtgcttgaat cgatgaaccg catcaatcaa
    13081 ctggcccgca tcggcagcct gcgctccgca atggacgaag cctatcatgc tgtgcaatac
    13141 gcgcccacct atcttcccct ccacgcgttg atgggtgatc tgctcgttca ggaaaaccgc
    13201 gatgcagatg caattgcaaa atacagcatc gttgcgcatc tttatggggt acgcggcgaa
    13261 gtaaatcaag ctacaaaact cctgcgccgc atcatccaac ttgcgcccat ggacctcggt
    13321 tcacgcaacc gcctgatcga ccaactcgtc gcaagaggtc atgccgatga agccgtgcgc
    13381 gaataccttg aactggcagc cattcactac cgcctcgccg aactcgacct ggcccgcaaa
    13441 acctacacca ccgcactcag actcgtacaa cagggaaatg ccaaccgtga ttggaatgcc
    13501 cacatcatgc agcgcatggc cgatattgac ctgcagcggc tggactggaa acaggcactc
    13561 cgcatctacg aacaactccg cagcatcacc cccgatgacg aagccacccg ccgccaattg
    13621 gtagacctca acctgcgcat ggcccaaccc gacaaagcca tgagcgaact cgagaactac
    13681 atcacctatc tcgaaagcca gggcaagaac gacctctcca tcaccttcat tcaagacctc
    13741 ctgcaggaac atcccgacca actcctgctc aaacgcgtac tggcggcacg tctgcacttt
    13801 gcaggccgca ccgaagaagc catctccatc ctcgatgtcc tcggcgaaaa cctcatgcag
    13861 acgggcgaca aacacggtgc aatggaagtc atcaaccaga tcattttgat gaacccaccc
    13921 aacgtatcag actaccgtca actgctcatc caaatgcaag gcaattagat ttcatccccg
    13981 tcagataaaa atggcgggga tttttttcgc ctctcactcg gaccgttgaa cctgatcctg
    14041 cttcccattt tccaaagaac ccccagcaga aacattctca cagcgaagtg ctataatccg
    14101 tgcgatttca acaacggaga acaataaaat gctaaaaacc caaaacaccg ccgtgatgaa
    14161 gtggctttac gtttttgcct tcgtcgtggc ttttctcgtc gtcttcgggg gatttgtacg
    14221 ccttacccgc tcgggccttt ccattgtgga gtggaatccc atcagcgggg ccatgccccc
    14281 cagcggacag caagcctggg aagaggagtt tgcaaaatat caggaaaccc ctgagtacaa
    14341 actcatcaac accggcatga ccctgcctga atacaaattc atcttctaca tggaatggtt
    14401 ccatcgcaat gttgcgcgtc tggcaggttt gttctatgcc ttccctgtgt tctacttcct
    14461 cttcaaaaag accattccct tcaaagaatt tggcatctac tttgtaatgg gcattctttt
    14521 catttcacag gcggttgcag gctggatcat ggttgccagc ggactggaca aacgccctgc
    14581 cgtcagtcac ttcaacctca ccattcacct gcttctggcg ctcacattgt tcagcctcgc
    14641 cctgtggact gccttcgggc acaagtacgg ttttcacgat accagcaaga aggcggcctg
    14701 gtcacttccc tccaaacttg ctgtattctc ttttgtcatt ctcgccattc aggtttcata
    14761 cggcgggttt acagcagggc tcaaggctgg gcatgtttca gatacctggc cattaatgct
    14821 cggcaaatta attcccccca acctgttcaa ctcgtggatc aatatctttg aatccccgca
    14881 aaccatcgtc ttcatccacc gctggttcgc ctggctgggg ttgatcctcg tccctgctgt
    14941 gtactatctc gttaaaaagc aaaactatcc cgccgatatc caaaacggac ttaaatggtt
    15001 gatcggtgtg gtggccctgc agatcacgct tggcgtgctc accatcctgt catacgtaaa
    15061 cattgtgatc gcgctgatcc atcaagccaa cgccatcgta ctggttgggc tggcagtgtt
    15121 cttcatccac aggttcaggg cagtggacag gaagtaaaag aaaaaaaagt gaaaataaaa
    15181 aagcgatggc agattcatct gccatcgctt ttttatttaa cagcccgaat tgttactcac
    15241 ggcggcgcgc ggccatgaaa gttgcgccga tcatgccaag ggcaaggcca accaccgaag
    15301 aagcccacga aaagaaattg atcaagaacg tagactcgat cacgtggatg accatcataa
    15361 agggcagggc aatgcccagg atcatcagga acaggccaag ggtcagaagg aagcgtggag
    15421 atctcattgc ggttgataca gccaacaggc cacaaaatgt tcgggttcag gttcaaaatc
    15481 cacgggttct ttttgatcgc acaaacccgc aatggcccgc gcacagcggg gatgaaaacg
    15541 acagcccttc ggcgggttca ccaaactcgg cggttcaccg ggcggcactt ccttgtatgt
    15601 atcggcattg cgggcatcgg ggtcggaggt ggcggcgagc aatgccatgg tgtagggatg
    15661 cagcgggtta tcaagcaggc ggcgggtatc ggctttttcg atcaggttgc ctgcatacat
    15721 cacaaaaata cgcgaggaaa aatatttcac tgtggaaagg tcatgcgtga tgtaaatgac
    15781 cgagagttga tgctgctcct gaagggttcg catcaactta aggatctcaa cccgcaccga
    15841 cgcatccagc atggatacgg gttcatcggc cacgatcaat ttcggcccaa ggatcattgc
    15901 ccgcgcaatg acaatgcgtt gctgctggcc gccgctcaac atgtgcggga attttggcag
    15961 aaagtcattg gcaggataca ttttcacttc atccattgcc ttgcggatgc ggtcaagccg
    16021 ttccgtttta tccttcacac cgctgatgat gagcggctct tcaaggatct gttgaatggt
    16081 catgaaaggc ggcagcgcgc catacggatc ctgctgaaca taaccgacct cgaaacgata
    16141 ttgccgcaga agattcccct ttaaaccagc cagattgact ccgccaaaaa tgacttcgcc
    16201 gctggtgggg atattcaacc cgaggatggt cttcatcaaa ctggattttc cgcatccgct
    16261 ctcgccgaca actgccacgg tttccccttc agccagatca aagctgacgc catccaccgc
    16321 cttgacgtag cccgcatggc cgaacccgaa gcgccgcagt tcgtaccaca catgcagatc
    16381 acgaatggat agcaaggttt ccttcggttc ttcaaaatta actgtcatct tgttcacctt
    16441 tcgtaattca gtgatcatgt ctgcattcat ggggatccgt cactttctat tttcagttgc
    16501 cgagccgaag cccgtttgca agcctttcaa acattccatc atgcccagat ggcggcaatc
    16561 atgcgttaga aaaaccagca agttcaagcg gatattctgt aatttctttt tcaattcatc
    16621 atccacatcg tcgaaatttg ggtaacgctc gtcgagccgc tccacaaatt cttccactgc
    16681 atcgtaggaa cgctggaggt aatccaccaa ttcctccttg cgtggaatgg gcagggtttc
    16741 caccgcttca tcactcaatc cggtgccaac cgtggcattc aaccccgttg ggaagcccca
    16801 cttctgcgcc aaaccttcct tcacccaaat ctccagcggg gtgccaaact ctggcaccaa
    16861 ttgaggtgtg cgttccaaaa tgatcgcttt gaggtaatcc gattcacgcg ccagatgcca
    16921 gagatgaaaa ccgatggagg tgctgtatcc gcgcgggcgc caatgcatct ggtcatcatc
    16981 cagtcgggag gcaaaatcaa gaatcacttt gtgaatatct ttgaacatgt agagaacgtc
    17041 ataaagagcc tgtggagaca tattcttcct ttttgtgttc tacgcgtgca gccagcactt
    17101 gaccttccga ttcgcttttt ggaaaacagg cggctcctcg ctgcattttt caaaacgata
    17161 cgggcagcgc gccgcaaaac ggcagccttt cggcgggttc atcaaacttg gcggctgccc
    17221 tgtaatgaac atcgggtctg aatcggtgcg caaacgcgga acacttgcca ttaacatctg
    17281 cgaatacgga tgcagagggg cgggaaaaaa atcttttgca tcgcctgtct ccacgatctg
    17341 gcccgcatac ataatggcaa cttcatcagc gagttcgctg gaagtggcta tatcatgcgt
    17401 aatgagaata aaggaggtgc caagctcatg cttgatgcgt ttcaacacgt tcatgatgtt
    17461 cgcctgtgtg agcaggtcca gcgctgaagt cggttcatcg aggacaatga gactgggcga
    17521 agtgacgagg gccatggcca acgccacacg ctgacgcatc ccaccgctca attcaaaggg
    17581 atagcgttcc acaaaatcgg caggcacacc gacatgttga aacattttgt aaaccagttt
    17641 gtaggcgtca actttgctca ggcccaaatg caccatggca ggttcagcca cctgatcgcc
    17701 caccctcaag acggggttca atgaattcat ggcggcttga ggcaccaatg acatgcccac
    17761 ccagcgcaca ttctgtcggt actcctcatt gtccagggtc attacatcgg taccctgcaa
    17821 atagacatgc ccggagtatt tatccacatt gcggggaagc aggcgcaaca aggcctttga
    17881 gagcgaggtc ttcccacagc ccgattcacc gaggatgacc acggcgcgat tggaatcgag
    17941 gtcaaagtca accccatcca cggcttgcac ggagccattg gcggttttga aatgcaagac
    18001 aaggttcttg atatgaagaa gtttctcagg cgcagcgttc atacatccct cagttttgga
    18061 ttgaagaccc ggtccagtgc gaaaccgagg aaggcaaagg ccaacccggt aatcatcagg
    18121 agcatggcag gctccaggac ccagtagtaa taccctttgt acaatgcgcc attggaattg
    18181 gcatcctgaa tgatcttgcc ccaggtgggc agcacagggt cacccagccc gatcacggcc
    18241 agtgaagcct ctaaaaatac aaaggcgggc accgattgaa ccaaacttgg aatcaacaac
    18301 gggatcatgc gcggaatcaa atacaagaaa atgatacgcg gactgctcgc cccatacgcc
    18361 ttggccgctt cgatgtacat cgattccttt acctgaatga agatggcgcg atatgccttg
    18421 atggaccctg tgaagatgct caagataatc gtcgcaccga ggatgaccca gatactgcgc
    18481 gaatagaaag tgccgatcat gatgagcagc gcatagaatg gcagcaccag attcacttcc
    18541 gtgatgcgtt ggatcaactc atccacccag cccgcgtacc aggcaccgat cgcggcgatg
    18601 accatcgtca aaattgatgt gcccattgag gcgatcagcc cgaaggccaa tgccacgggg
    18661 acaccccaca gcaatggcag gaccaggtca cggcgcgcat gatccgttcc cgcccagccg
    18721 aagacctgtc catgtgaaac aaattccaca ttcaagtcag agtcaggttc gaaggtcgtc
    18781 cctgtgatga tgagtttgta tttgcctttc aagggctggt cgctgccggg cagagagaac
    18841 agggcgggga tgacctcatc cgcacgtaat ttggtcagca gtttttcatc ctgcgagaag
    18901 cggtaggtga actcattgct gacagagaag tttccaatgc ggatggtacg ttcatcgggc
    18961 gtgagcaatt caacagagat aaaaggttgt ttttccttgt atgtggtcgt gacgtataaa
    19021 atcagatcct gcggatacac gtcgtaggaa taatcaaact cataggccat atcgaaggtt
    19081 gaaatgcctt cattgcccgg ggtgacggtc ttcgtcatct ggccgtcttt ggtgttgacg
    19141 gcaaacgatt cggaatattg tttgctggaa aaataattga tccaggcagg cgcggcgaac
    19201 cgcggatttt gataccagac ctcctcccca ccgcgccaca aacgtacggc ctcggtatag
    19261 ggaatggtga tcatggcata ggcggcaaca aacaccaggg caaacactac aaaaatgccc
    19321 gccactgccg atggatattg aaccagtttc tggaacgaac ttctaaaatt attcatattc
    19381 ctcagccgcc tatcttcacc cgtggatcaa ccagggcgta aataaaatca agcagaaaaa
    19441 cagtcatggc aagcatgtag gcaaagatga tggatgagcc gacgatgatg ggcgtatcgt
    19501 attgaccgat ggcttgaaaa agcgtacgac ccagccccgg ccagaggaat acagtttctg
    19561 tgaaaagggc acccgtccac agggtgatga tgagcaggga aaagttggtg ataatggtcg
    19621 gcagtgtggg gcgcaggatg taggtccgct cgatatcgcg ggtgggcagg ccctttgcct
    19681 ttgccatatc cacataatct tcgctggagt agatgaggaa aaaggtgcgg tagttgtaaa
    19741 cgctcaaaaa gaacgaactt agaatcagcg ccaggcaagg cagggtcaag tgcttgagca
    19801 cactcattga ataatcaaaa gtgttgtcag gcgggggcga atccaccatg ccgccaaagg
    19861 gcagcacttt caaagccgca gcaaagatca ggatcagaaa gatgccataa aaccagggcg
    19921 gtacggcgga cgtaggtgaa agcgcgatga ccagcttatc caggaaactg ccgtacttgc
    19981 gcgaaagcga aagggcaata aagatgctgg aaaagaacag gaataattgc gaggtaccca
    20041 tcaacaacag ggtggcaggc agccgttcga gcaggatcaa acgaacctgt ttggagccgc
    20101 tatcgctcgt cagattgatg gcgcggccaa ggtccaattg cagggcattc gagagataac
    20161 ggaagttacg cactgcaatg ggggtatcca atccgcggcg tttttcttcg agggcgatct
    20221 ggtcttcgat caatttcttg cgcacatcgg gagccatttg caaggcggtt ggattgttcg
    20281 cgatcttttg cgtcaccgaa tcgcggatct gtcctttgat gatctcatcc acaaaaccgc
    20341 ccatgttggc gatcaagatg gtcaggtaga ttccgatcac cacggtgatg aaaagcgtca
    20401 acaaacgctt cgctgaataa cgcaggatgc gtgcaaagga actgttgaga aatgattttt
    20461 tgacgaccac attctccaaa ggttgagcgg tagctgacat gactgcctcc acaatcgagg
    20521 gttcagggcg gagcgggtgc cccgccctga acaaatcact tacttgatga caacgaaatc
    20581 gaacgatgtg aaggatggga tcgccacagg aatcgggacg acagccactt ccaatttcgc
    20641 tgaaccagtt tgcagtttgg atgttgcttc cgcatccaac gtcacctgga actgcccctc
    20701 gccaacggcc tgcgcctccc cgacggagac caccgcgcca gtggtgtcgt agagaatgta
    20761 cttcaccagc ttgatatcgg catttgcgta ggcggcatcg ccaaggttga ccgtcacatc
    20821 aaagacggct tcctgaccag cagtcacctg cccggggcca tcgaggatgg tgcttgccag
    20881 tttgggcgcg ctaaagcgtg accagcgatc ggaggcatcc acatactcgg cgttgtgttt
    20941 caacacgagc gatttctcgg tcgtaaagac ctgatcgaga tagtacggcc cggtgccaac
    21001 ccagaagtga ccatgatctt cgtaccactt cttcatgttg gcgtaacgct cggtgatctc
    21061 ttcggcggtg aggtattgac ccagagtggg ttcgtacgga atcaggtttt cagactgtgc
    21121 ctgatccatg tacttctcaa ggatttcaag gctcggcccg cccacccagc tcatttgctc
    21181 gatctgggct acatcggctt tgtcggagga gtacgcgagt tcaccagcgg cttcagcaag
    21241 gttcgaaaca gccaaaatct gccagctatt ttcaccggaa agaccaaagg gagaattcgg
    21301 ccaaagggtg aggatgttca attccgcatc ggcattgtag gaatccgagt aggcttcaat
    21361 ggtcaacgga tcggtcgaaa cgatgcggta tcccttgaag gatggcagga agcccgccag
    21421 accagggaca gccgcttcat cgtaaacata gctttcgggt ttggcgcggt caaagaaaat
    21481 gatgatcgac atgacaaagt cggctacaga gatggaactg ccatcatgcc atttcacagt
    21541 gtcataaagg tcggcaggga aggtgacaac actcttcact ttggccgtgg tgccatcggg
    21601 gaatttctcg cccgcggtga tgaatttctg gttggcggcg tcccaatcca cccaggcatc
    21661 ttcgggcaca tcaatttggt ccacagcttc cacgctcaac cagtcgaggt tgtgggtgat
    21721 gggcaggccc ttttgatagg tcaacgaagc gctttcgatg cgctgcggcc aggccaggcc
    21781 tgtgtatgga tcgcccatga tgccgccagt gccggtgatg ctatctgcgc cctgcgtagt
    21841 ggctcgcatg acattcgaat cccataccca gttactgccg ccgatggtat tccacggctg
    21901 ggtgaagaga tcgttggtgc cgatcttcat ggtgccaccc tcttgatctt tgaagcgcag
    21961 gttgtagttc cccataaagg tcgattcata gccagcggcc agatcgtagg tcacagaaac
    22021 attgctgttg aaaggtgcat aggtctgctg gtcaatgacg aagacgaaga gagaatcttc
    22081 cagagaaagc cgtaaagcct ccgccatcat cgcgtcacgt tcttccttcg tagagaaatt
    22141 acccaaagcc aggtcatcgc ccagtttctg gaattcaggg tcagaaacgt tggagaggaa
    22201 aggctcggca gcctgcacac tggtgttcag gtaatattgc tggaagttgg cgcgctcatc
    22261 gcgggtgaga cctgaaggca ggtacccagc ggtgtacaga ttccattggc cgtctttcgc
    22321 atcggtgccc tgccagatcg ggaaggcttc ggaagaggtc ttgtactgac gatcaacggt
    22381 gaaaccaatg gcttccaatt gattggaaac ataatcaccc atcggctggc gtgtgccatc
    22441 tccatcagag cggatcagga aggccagtgt tatgggtgcg ccgttgtatt gccacttgcc
    22501 atcggcaccc aattcagcac ccatcccgac catttcggcg ctaatcactt ctttggcctg
    22561 atcgaggtta taggcatatt tggtttccaa tgcacgggcg gtttccacga ggttggtata
    22621 ttccaccaac tgggtggtga tcgagaacaa tttcggcaga gagccgccgc cataaatttc
    22681 ctggttgatg tagttacggt cgatcagcca gttcatcgct tcgcggatct tgcggttcga
    22741 gaacggattc aacactgcgg tatcggtgaa tacagcagga ttcatgctga tggcataact
    22801 tccgccgaac gactgggtat agcccagccc ggcttccttg atggctggga aagcatcaga
    22861 agcaagggca aaggagtaaa aatcaattgt ccctgcctgc agctgcgaga tcgcagaatc
    22921 agccgcaacc acagaaaaat caatttcgtc gagccagccg ccccgtcggt cagtggcggg
    22981 cgccgcaggc gcttccgtgg cagcggtggt gggaggttcg gttgcggcag gcgcatcggt
    23041 gggcgctggt tcttcaactg cttcagtagc agcgggtgcg caggctgcca ggatcatgct
    23101 aaaaaccaca agcagactga acaacgcata taattttttc agtttcattc tcttttctcc
    23161 ttggtttggt tttgaacaaa tgaaattgca tacaatggaa gatctacaac caaccacctc
    23221 ctttgtcttt tattcagcag atagaatggg aggcggagtt cagtgcgtaa ttctgaaatc
    23281 cgcctcgcct gtcaaagagc ggtatgcttt cgtgaggaga aggctgcccg ccaaacttga
    23341 actcatgata cgaattcccg ctgcaactgt catgcacctg cataagcatc tttgccaata
    23401 cacttgaacc gttcgtcaac catgccaaac cacccgcacc ctgctcccaa ggcaataaaa
    23461 aaaccctccc agaggagggt attcaaacag gtggggcggg cccaaactgc atataattga
    23521 actgaccatg cgaaaatact tcgtctacgc gattttttta ctcaccctgc tgggcaattc
    23581 ctgtgttgcg gcgcccgaca ccttgaagcc cccactgccc gttgtattcg acgagccaca
    23641 atcgggcttg aattcatcgc gcaacagccg ctcaacccca tcctacagcc agaacatccg
    23701 cttcgaacat ttcagccttg aagaagggct ttcgcaaagc acgatcacgg tcatctttca
    23761 agacagcaag ggcttccttt ggatcggcac ggaagacgga ttaaaccgct acgatggcta
    23821 tactttcaaa atctacaaac caaacccgga agacccgaat accctcacag accgctgggt
    23881 cacagcactg gcagaagacc ccgaaggcaa cctgtggatc ggcacacgcc tcggcggttt
    23941 gaatcggtac gaccccgaaa cgggacaatt ctcacatctg ctgtatagca acacgaacag
    24001 cgcaggcatc agtcacaatc acatcaccgc tcttagcgcc gataaagatg gcatctggat
    24061 cgggacaaaa aacggtctgg actacctctc atatggttca caaagaatcg agcatttccg
    24121 cgcctcatca gtcaacccca acagcctgag cggcaactgg atcagcgcca ttattcaaga
    24181 tgccaatggc aacctgtggg tcggcacaca agactcaggc ctgaactttt acgataaaac
    24241 caaaaaatca ttttccgttt tcaaacacag tgacgacgtc tttaccagca tcagcaacaa
    24301 ccatatcacc agcattttga tcgacaaccg tggagtgctt tgggttggca ccgccaacgg
    24361 attaaacaac tccgaatcgg cgggcagata cttcacccac tatgtcaatg tcgatggcga
    24421 ctcggacagc ctgagcggtg acgttgttta cgcgatctat caagaccgtt ccctcggcat
    24481 ctggatcgga accgacaacg gactcaaccg ctacgaccct cagcgcgaaa gttttgtgca
    24541 tcacaaccat cagcccacca cacccaacag ccttgcaaac gatacggtga tttccatctt
    24601 tgaagacaac agcaacgtgt tatgggtcgg caccgatggc ggcggcttga acaaatacaa
    24661 ccgtcagcag gaccattttg cttattaccg cgccaacccc gatgacccca ccagcctgag
    24721 cggaaacttc atcatgccca tcgttgtcgg cgaaaatggc actgtttggg tgggcgccta
    24781 tggaacaggg ctgaaccgcc tcaatgccat ttccaatcaa ttcaggcgat atgtgcatga
    24841 ccccaacaac tccaactcga tcagcaccaa cgatgtgacc gcccttaaaa tagacagcag
    24901 cggcaacctg tggatcggaa cctccatcgg gttggatcgc ctcaatgcgg atacaggtca
    24961 attcactcat tttgtccccg atatcaacaa ccctgagagc ctctcggcgg cccccgtcaa
    25021 tgtcatttat gaagaccact cgaacaccat ctgggtcggc accagccgtg gtctcgacca
    25081 gtttgatccg cagacacagg tctttatcaa atacgatacc gaagataacg acccaaacag
    25141 tttcgacaac aatcagatca ccagcatgct cgaagatgaa aatctgaatt tctggatcgg
    25201 caccttcgat gatggcatca aacgctttaa tccgagcaac ggcgaaatca ccagctacac
    25261 caacaatcca gataatccct ccagcctggg gagcaacgcc gtcctatgcc tgcacattga
    25321 ccgggcaggc accttgtgga tcggaaccgc aggcggtgga ttaaaccgct acaaccccga
    25381 aaccgactcg tttgttcgtt ttaccgaaaa agacggcctg cctaatgatg tcatctatgg
    25441 cattctcgaa gacaaaaatg gttttttgtg ggccagcacc aatttcggta tcacccgctt
    25501 tgaccccaag accaacacct tccgtaattt cacggccagc gacggcctgc aaagcaatga
    25561 gtttcatcaa aatgcctttg caattgacct gaaaggcaac ctctacttcg gcggtaacaa
    25621 cggctttaat gtctttacac ccgatgccat tgacagtgat accgtggcgc caaagatcgt
    25681 actgacctca ctcacgcgcg accgcacccc gatctccaac aacatccctt ctgaaagtat
    25741 caaggagatc accctgcaat ggccgcaaaa ttcctttgaa ttcgaattcg ccgcgctggc
    25801 ttatgagcag ccttcgaaga atcaatatgc ctacatcctc gaaaacttcg aagccaattg
    25861 gaacatgatc ggcacccaac gcaatggacg ctacaccaac ctgcccggcg gctcctacac
    25921 cctgcacctg cttggcgcca acagcgatgg ggtctggaac aaggaaggcg tctccatcag
    25981 ggtcacggtc atcccaccca tctgggaaac ctggtggttc cgaagttccc tgctggtttt
    26041 tctggcagca tccgtatttt taggataccg ctggcgtgtt aaacgaatcg aagcacgcaa
    26101 ccgcgaattg gaacgtctcg tccaaaggcg caccggcgat cttgaaaaac gcacccgtga
    26161 aatcgaagcc ctttatcaag cggacgaacg gattttacgt aacgtcaccc tgattcaggt
    26221 tttccaaacc ctggtggatg tatccgtttc catgcttaag gcagaccgca gcatggtttt
    26281 catttggaac gaggaaaaga acaagatcac cccgcgcgtc agttatggtt tccgcactga
    26341 aacattgtat gcccttcatt tcgaccaggg cgaaggactg gtcggccaaa ccatgaaaac
    26401 aggcgagaca ttgatcgtaa tggatatcaa acgcggcacc ctgcgcggcg atgtggaaac
    26461 cgtcattcgc agggaaggga tccaatcatt tgcccacttc cccatcgtgg tagatggcaa
    26521 tgtcatcgcc atcttcaatg tagcctacac ccgtccaaac gcattcaccg atgacaccat
    26581 ccgcctgttc acatcgctgg tcaatcgcgc tgcattatcc atcgccaaca tgcagctctt
    26641 cgaacagacc aaggatctgg ctgtgatgga agaacgtaac cgcctcgcac gggacctgca
    26701 tgatagtgcc aaacaaaagg catttgccgc ccttgcgcag ttgggtaccg ccaatggcat
    26761 cctgaaaaca aagccgaatg atgtgaaagt tcacctcacc gaagcagaga ctcttgtttt
    26821 cgaagtcatt caagaattga ccttcctcat tcaagaaatc tacccgatcg ccctgcagga
    26881 aaaaggattg cccaccacat tgcgtgaata catctttgaa tgggaaaacc gcaacgatac
    26941 cgaagtcaag ctgacgattc gcaacgaacg tccgctgccg ctggagatcg aacaggccgt
    27001 ttaccgcatc attcaggaag cactggccaa tgtggcccgt cacagcaagg ccaaacgtgc
    27061 agatgtatcg ctggtttata attccgactc cctgcagatc atggtggccg atgatggtca
    27121 cggcttcgac atgaatcagc gggccaaagg catgggcttc cgttccatgc gcgaacggat
    27181 cagcagtgtt cgaggaacca ttcaaattca aagcgcgccc ggccaaggga ctcgtcttat
    27241 tgcacaatta cccatcaaat caccgggtaa ggaggcttca acatgaaaca ccatcgcacg
    27301 agcatcttaa ttgcagatga ccatgaagtg gtccgaaatg ggatccgttc ctatctcgaa
    27361 acactttcag attttcaggt cgtcggcgaa gcctcttcag gagaagaggc cctgctcatg
    27421 gtcgcagaat tgattcccga tatcgttctg cttgacctca tcatgccagg catggacggg
    27481 atcgaaacca cccgccgcat caaacagacc agcccgcgta cacaagtggt tgtgctgacc
    27541 tcctaccatg aggatgtgca tatcttccct gccttgaagg ccggggccat tgcctatatt
    27601 ttgaaagaca tgaaaatgga gaaactctct gaagttctac atcgcgccgt acagggtgag
    27661 gtgaccctgc atccgcgggt ggcggcacgt gttcttcaga acattcgcgg tgaaaatgga
    27721 gaggagcagc ccctgtttac cgatttgacc gaccgcgaaa ccgatgtact gaaactcatc
    27781 gccaacggcc tgaccaacag ccagatcgct gaaaaacttg tcatcagtga aaacaccgtc
    27841 aaggggcatg tgagcaacat cctaagcaag ttacacctgg cagaccgtac aaaactggcg
    27901 gtgtatgcct ggcagcaagg cattgtcaat agagacagcg ccccacggga ataaacccgc
    27961 ctgaaacaaa aaaggacacg gtctttcgac cgtgtccttt tttgttttta gaaatcctat
    28021 tgacccgctg cagccaaagc cgcatcgatc gcttgcgcga atacatcaaa tggctgggcg
    28081 ccttcgatca gttgggtcac ttcttcgccg ctggcgttga catagctcaa gacgaagcca
    28141 ggggtaccgc gcacaccggc ggtcagcgca tccttgccat cctggaagac ctggtcgtca
    28201 aacttgccgc tggaatagca ttcattgaat tgggtcatat ccaccccggt ttgttcggca
    28261 atggcagcca aacggcggtc ggtaaaggaa tccacatctt caccaagcac attggcaaag
    28321 agcatatcat gcatttccca gtacagattt tgatcgcctg cacaataggc agccttggca
    28381 gcatcttcag attcggtctt gccgccgcgg atgttgtcac ttacccagtt ccccgcggaa
    28441 cgataggtaa agtacagctt gccagtggaa acataagcct cgaccagttt tgcttctgtc
    28501 tcagaagcga atcgttcaca gaacgggcac tgataatcgg aatattccac gagtttgacc
    28561 ggagcatttg ggtcacccat gttgttggca tcagcttgcg gacgcggatt tggctcaaca
    28621 gagataatgc cagtaatcgg tttcaaatta ggccaaatga tcaaaaaagc cacagccaaa
    28681 gcaccaagta cgaccaagcc gatgccgata aaacgctggc gctgagactg acggcgtatc
    28741 tgctcacggc gcgcctgccg tttgctcaat tctttcgggt tattcatatt ctcttactct
    28801 cctattgttt agatggatgc gaattttccc atgctgaaat caatttgtcc agtacagggt
    28861 taatgggctc ttattttcac tcacgcaggc gggcatccat ccacagcgcc atggttttga
    28921 aaacttcggc cttttcaggc tcattgtgca gttcgtgata cgcaccatcc cacaggacga
    28981 gggtgcagcg ttctttcagc ggggcggcga attctgaact gccagcggaa aaggttaggt
    29041 gatcctcctt gccatgcatg agcaggaggg gcaatgggaa ttcactggca tgtgccaggg
    29101 tccagcttgt aacgccgatc atgatctttc caaacccaaa agacatctga tcatgcacca
    29161 acgggtcgtt gatataggcc tgaattactt tttcatcgcg ggagatggtt ttcggatcga
    29221 gtccactggg aatggtcaca gatggggcca cagaccccag cacctttgcc aacatcacct
    29281 tgaccttttg atcctcgagg gcggtacgca gaccagaact ggtggcgatc acacccttga
    29341 tattgggctt tcgcagcagg gtgtagtgca aaaccagcac cccgcccagg ctgtgaccat
    29401 acaaaatgat gggaaggttg gggtagcggt tgcgggcctg ttccagcagc tgatcgatga
    29461 cagtcataaa atcttcgatg gagttgatat gtccacgttt tccaccagaa cggccatgtc
    29521 cgcgcaggtc tgggccaaac aggatcaacc cttccctgct aaaagcctct gccacatgag
    29581 cataacgcga ggtatgctct ccaagaccat gaacaagaca aaccacgccc ttgggctgca
    29641 gcacggttgg ttcccaaccc tgtgcaaaaa cttccaaacc atcatgagct ttccagctcg
    29701 tttcaaagtg cttcacgctt ctcctccaat gctgatcttg aattcacatt gccgtgcgcc
    29761 catggctcga caggcgcgtt cttcaatatc gtgttcctgc cctaccgccc aaaacaaaca
    29821 ttcacggatc aggccgaggg taacaaaaca gatcggggct gtatcggttt gctccatcgc
    29881 cgtgggggat gcatggtcaa caaagagcag gtcaaggtcc agagaatgaa ccgagacatt
    29941 tcccgatgtg gcgcttaaga tccgtgccag cagttccaaa gcaggtttac gccgcaatcc
    30001 ttttggaagc ctttgtataa tggcggcctg cgctttaatt ccaaaggcgg aatcgttcaa
    30061 gagttgttcc caaagtttta ccccgatcca taacagggtg ctgcgcgcac cgcggccata
    30121 atacttgcgc aaagcagatt gcaggcgtga gtacacattg gcggcacgct ggtcatccaa
    30181 tgcagaaaaa tgcgccgaat gcgcccactc tgcaggcagg cctgcattcg tcaatgcacc
    30241 ggtcaatgtt tcgtggccca tttctgcaga aagggtttct acgaatcttt tcaagatatg
    30301 aacggggaaa ccgatctctg tcatcaggca cctatcccaa tgggtaaacc aacactggaa
    30361 aatattcacc agtgcggacg aagcccatct ttttgtaaag tgcaagtgaa gcttcgttat
    30421 ctgattgtgt gttgacggaa agacgatgca attgggcacc accaaacgat tggatcaggt
    30481 cgctgacgag ggcagccccc acccctctgc cttgcgcttc aggactcacc ccaagcctgg
    30541 ccaggtgtgc cccaagcgga ttcctcgtac tgagttggta cccgatcacc cctgaatcat
    30601 cttcagcgac cgtggcactt gcgcattgca atcgtgcccg ttgaagggtg cttgctgtgt
    30661 tgtgccagaa atctccgaaa gcggccagat ccaatttggc aaccatggga atatcggcat
    30721 cggtcatcgg tctaatacga accccgtgcg gggttagaaa gtatcgaatg tcttcgtttc
    30781 gcaattcaag caatacgatg ttctgttgat tgataaatcc actggaaagc aaaagcggtt
    30841 gaaaccattg gcggacaacg attgcggcaa ccttgagcgg cctgttctcc gatgcaagat
    30901 cactgcgggc tttttcccag agcaatgacc acgcttctgc cccagagagg gaggggctgt
    30961 atccaaaaaa gctgacccaa gccacgttag gcgggtcttc agggcaaatt aaaacagcat
    31021 ggatgcttgc accttcagca agcacccagc aattcggcag gttgatccgt tccagcggtg
    31081 aatgccaatc caaatggcgg tggttgttgg cttcgtagta gacaaggctg gagacctggt
    31141 tgtaatcttc agcaaccatg cggcgggcct gcaagttgat attgatcatt tgataaagcg
    31201 gagaaagaat ttaagaatgc gttcgaagaa gccaggattg tccttggcat caagagaccc
    31261 catcattttg aaggccgatt ctgttacctt gcctgttttg agcttgattg ccgcggcaga
    31321 tttcatgacc atggcacgaa ggtcgccttc cccaacttcg gcaaataatt ctgcagaacg
    31381 ttcataggcg gcgatggctt cgtcaaaacg attgagcgct tcgagggcgg cggcttgatt
    31441 ccccaatgcc attccctgac ttttaatgtc cttcgcccct gcaaagacca aatcggttcc
    31501 caatgcggca tccagcgact cttgtgcttt gcccgcctgt aacaatgcca cactcatgtt
    31561 gtttttcatt tcagcggcga gcagcccatt gcggccaagt gaaaacccct ccgcggcctg
    31621 gcggaacaat tcggcggatt cgtcaaattg tttgtttctg aaggcctgct tgccttgttc
    31681 tgcgagttgg gcgggttcga ttgtcatggt catttcccaa aatcaagaat gttttgcaac
    31741 gatgcaggga atggtaatcc gctaaagcgt gatatccaag agcccttcgg caacagagcg
    31801 caatttttcc ttggacatgt cgctgagttt catggccaat tccaggtagg ggcggttgac
    31861 cggctgacag acaaaatttt gaatctcttc tggcaattcc aaaaactttt gcatggcccg
    31921 ctgactggtc atccactggc ctacggggcc attctggtca aagaaggttt caatgcggct
    31981 gcccaaaacc gccagaatgc tctccaactc agggacggag atcgggcgtt ctcccaattc
    32041 atacaggttc agccgtgcgg atttgatccc cgtatccact gcaagctgcc gaagcgacat
    32101 attgatcttg ttacgttcct gtcgcaacaa ggcaccgatc attctttgac gtaatgcgat
    32161 caactgtgcc gtatcaagag tctccgccgg ggaggatgtc tcggacatgg tttcccgtcc
    32221 ccagaagtga gtgatcggca acttcagata ataaacaagc gtttccaact ccggtaggga
    32281 tggggaacgg cgcccttctt cataagcacg gaataacccc ggctttacac cgatggcatc
    32341 cgcacattcc ttgatgctgc gtcgttcggc catacgcgca tcacgaatga gtaaaccaag
    32401 ctttttttca cgtatgatga tctgggcgtt attcattatt cctcgtaaat agggttacag
    32461 gatatttttt aatttgggtc aagggtcatt ttcgccagca atgctcaacg tggaatttat
    32521 tttgctgcat ggcgggcgaa caggcctaga ttatagcaga gtgtttcagc gccgagagcc
    32581 ttgattggcc gacgcttttg gcgcaaagat atcagcagtg agcttgaacc cagccgcttc
    32641 aaggcggggt gtgaagttgg gacgaatgcc tgtggcactg atctcgccga gcacttttcc
    32701 ttcggtggtc acgccggttt gaataaactt gaacacatcg gtcaacacga tgatctcacc
    32761 ttccatacct gccacttcag tgaccgctgt caccttgcgc tgcccatcct ttaagcgcgt
    32821 ctgttgaacg atgagatcaa cagcagatga aatttgctgg cgcaccactt taattggcag
    32881 gtccatgcct gccatgagca ccaaggtttc aatgcgcgag atggcatccc gcggggtatt
    32941 tgcatgtacg gtggtgagtg aaccgtcgtg gccggtgttc atggcttgca acatatccag
    33001 agcctcgccg ccacgcacct cccccaccac gatacggtcg gggcgcatac gcagggagtt
    33061 tttcaccagt tcacgaatgg tcaccgcatg aagtccttct gcattggcta cctttgtttc
    33121 catccgcatg acatggtcct gctggagttg tagttcagcg gcgtcttcaa tggtgatgat
    33181 gcgttcgttt tcgggaatat accccgaaag cacattcaac aatgtggttt tgccagaccc
    33241 cgttccacca gaaatgatga tgttgaagcg ggctttcaca caggcttgca aaaaatcagc
    33301 catttggcgg gtcaatgaac cgaaattgat cagatcctcg ggctttaact tgtctttcct
    33361 gaatttacga atggtaatgg aaggcccatc gatggccacc gggcgcacca ctgcattcac
    33421 gcgcgaccca tcgggcatgc gggcatccac ggtgggagag tctgcgtcta catggcggcc
    33481 aagcggcagg atgatacgat cgatgatacg catgacgtgg tcatcgtcat cgaaggtgac
    33541 cgtggacttg gtcaactgtc ccttcttttc aacgaacact ttcttggggc cattgaccat
    33601 gatctcggaa acatcctcat catccaacag ggattggatg ggaccatatc cgagcacgtc
    33661 gttcaatacc tgttcaaaga tctgttttcg cagatcctct ggcagtttca attgtgtctg
    33721 ttcatacaca tggacaatgt tctttttgac aaaggcagag cgttcagcag cgggaacccg
    33781 ctcggaaccc attgcactca tcaagttatc ggcgagatat ttctttaagc gggagagatc
    33841 ctgtccggtc aatctgctgg atggggtaac ggttgtcatg atcaacctcc ctggctggat
    33901 tcatctgcgg gggtgatcca tatgatctgt gcgcgcaaaa ccgccagaaa cgccccggag
    33961 aaaagcacct tctcgtttgc atcctgaatg gtgaaattgg ttacggcaat aaacctttca
    34021 tccatttcca attcatcttt gagcctttcc ccctgacgga catgtaccag cccacgtatc
    34081 aggtgtgtgg tggtttgtat cacaactgcg gtggggactt tggtcaccac ttcggtgtag
    34141 aacttgccct tctcatcgta ttcaattgtc atgatgcttt tccttgtgac cgcccgcaat
    34201 gcaagcggtg ataaaaagag gctttggtgc tcgccttaca tggatgcagg tgtaacggat
    34261 gtacacctgt atgatcgatg aagcacgtct attataagtc tattgacctg ttcgtagttt
    34321 ttgagcaaga accgccattt ccttcgcggt ctccaaaaag acaaaggaag ccgtatcgtt
    34381 cgggaatttt aaagaaaaag gttgatgatg gctggaggcg aacgcgaaac tgctgccaag
    34441 gtagggcatg gccgctttga tctgaatatc caatgccttt tcagcatcgg gcttgctcaa
    34501 tccctccaat ccgctggctc ggttcaagat cggaaagatc gtggcaggct ccacaccctt
    34561 gattttcatg taatccaaca cggtttttgt gagcggcagg gcactgacat cggtactgat
    34621 gatcagggcg accagatcgg cgtgctgaag gatcggaagg ctgatcttcg aaagtgcgcg
    34681 gccgatatcg atcaacacat aatcatagga agctttcaaa gcagcgatca cgttccagat
    34741 gcgccccact ttgagatgat tgctgctttc agggtcaggc gaacccgaaa gcagatgaaa
    34801 acgccagatg ccagcctctt tcaattgatc gcgaaaataa tcgggggtga tcttttcttc
    34861 gggcatatct gaagctgtga cgatgttcaa cgatccttca tagccgacaa tggatccaat
    34921 ggacccaatg ggcagaacca aatccaccac agccacacgg gcttcgggtt gattttggca
    34981 gacggtcaaa ccgagatttg cacacaacga agaagttcca actccaccct tggcgctgat
    35041 aaaggccatt aacaaaccgc cttcctttac aagcgcggac gatattccca gcaggcggtt
    35101 aatcgcatcg gtgagggtgg ccatggcctg gcctgatttt gcgatgtatt cgctaaaccc
    35161 cgcatcaagg cacgacttga tgcgggccac ccccagatca ctgctcaatg caatgagggg
    35221 catattcgta gtgcgggcat cctgcttgag ttttctggca acctcctccc cggtaatatc
    35281 cgggacggtg gggtctatta ccatcaagtc aggacgatcc cgccatgcat ggatcaatcc
    35341 ctccttacct gaaccagtct gaatgatttc atatttttga gcctgcagat tacgtgaaat
    35401 aaaattacga ctggcgacat ctgcatcaac aataaggatc ttcttttcag acatgattca
    35461 aattcctttg cgcgtgtggg tcacgcgcac cgccgggtca aatttctgtg ggcggcatca
    35521 agtatcccag accggagcgg gtcacaatgt ggcgcggaga atgcgcatcc gcctccagtt
    35581 tctggcgtaa acgggcgata ctgacccata agatttcctt gtcgttcttg tactccacac
    35641 cccatacact ggttaacagg tcttccgagg tgaggatctt tcctacgtta tgcgtaaatt
    35701 gcagaagaag gcggtattct gtggcagaaa gcgaaactgg ttcttcaccg cgccatactt
    35761 cggcacgtgc aaaatcgatc ttgaggtcat catgtgtaaa gaagcgggcc tgccccgatt
    35821 cggtgggcgc ttgtgcccgg cgtaaaacag cccgcacacg tgccagcaat tcggtggcag
    35881 agaaaggctt caccaaataa tcatctgcac caaggtccaa cccacggaca cggtcctgct
    35941 cttcgccgcg tgccgtcaaa atcacaattg ggacgttcga gaattcccgc aacctttggc
    36001 ataccccaaa cccatccaac cgaggcatca tcacatccaa aagcacaagg tcaatgggtt
    36061 gtactgaaaa cacatccaaa gcctgcacac catcctgcgc tgtggctact tcataacctt
    36121 cagtacgcag gtttgcctcc aacaggcgca aataacgggg ctcatcgtca acgatcaata
    36181 tacgcttgga cataacgatc tccttgagga aagagttgaa ggttggtcaa agcgttgaat
    36241 cgacaggcag ttcaataatg aacgttgtgc ctttatccag ctctgactct acccaaatat
    36301 taccatgatg cgccataaca atttgcttgc aaatatacaa ccccaaacca gttcccgtta
    36361 cggtacgctc accgggcaca cgatagaaac gttcaaaaag aaaggggaga taatcttccg
    36421 ggatgccctc acctttatcg gcaaaagaga cgcggatctt atctccctgc atgcgcatgg
    36481 cgatcgtaat ttcggaattg ggcgcatatt tcaaggcgtt gctaaacaga ttttcaaaca
    36541 cctgtgcaat gcgcattcca tcaccgcgaa tggagggcag tgaatcggtt tcaaaattga
    36601 ccttcaattc agggtgatgg gtatggatgc gcatggctac atcacgagca agcgcatcga
    36661 ttcggatggg tgaaaattta aattgcaggg tcttgctttg caggcgtgcc gactcgagca
    36721 tgttctcaat caatcgtgcc aggcggtcgg cttcttcatc aatgatgttg agaaactcgc
    36781 gctgagtggt ttcatcccag gtggtatcct gcctgagcag gctggtgctg taacctttga
    36841 tgaacccgag cggggtgcga agttcatgtg aaatggtgga gacaaaatca tcctgcaaac
    36901 gcatctgcct ttgcactgaa tccagttcgg tgcgcgcctc ctgcaggtct ctgcgttcga
    36961 tcagggccgt ggcccaaaac gactgcaaag atgcgatctg aatatgcagg ttggaatatt
    37021 cagggccgcc aaaacggatg aagaccagag cgccattcaa ttttccgccc acgtacatgg
    37081 ggaaacccaa caaatgagcc tcggtcaggc ggttggcata attgagattc tttctcggct
    37141 ccttgaggat cattctgcct tttgaaagca cctcatttgc aatgccctcg ccccacattg
    37201 agtctgcttc ggcggctttg ccgcgcccca ctgcacgggc atacacaact tcgagaccct
    37261 tcgtgcgcgg gtcttccaga tagatggcgg cattgtcaaa tacaaaagaa ccgcgcatgg
    37321 atacaaacaa cgcatccaac gcggctctcc aatcctgttg tatctgaacc tcttgaaagg
    37381 tcttgtgcaa aaatgttagc gtagccagat ccataattag tcacccaggg caaggagcgg
    37441 gaatttgaaa acagagcctt tgccttctgc tgattgtaca tccaataacg atccgtgggc
    37501 ttcaacgatc tgccgcacca gtgaaagccc caaccccgtg ccgccgaaat gccgcgttga
    37561 agaaccatcc aattgatgga atggttcgaa gatctctttt attttattgg atggaattcc
    37621 aatgcctgta tcggtgaccg taaggacaac gagattgtga ccttcctctt tgagggagat
    37681 caccacgctt ccacctgacg gggtgaattt gatcccgtta tcgaggagtt gactcaccac
    37741 ccagccgatc ttctcagggt cggcctgcac agatggaata ttctccaaag ctgagaccag
    37801 cacctgtaca ccgcgttcct cggctttggg cagggccgat ttcgcagcca gcgaaacgat
    37861 gcggcggata tccactcttt gcagtttcat gctcatctcg cccttcgaag ccagcgagaa
    37921 catgatgagg tcttcgatca tgccttccag ttttgccgtg gcccgttgac tcacctgcag
    37981 ggcatggcgc tgttcttctg agatttcacc cagcgaccca gccaccagca attcgatata
    38041 accttttaca tgagtgagcg gtgtgcgcaa ttcatgtgaa atgttcgaaa caaaattcga
    38101 tttcaactgg ctgagttccg aaagtctttg cagggcctca ttcaattctg ccgtgcgttc
    38161 cttcacgcgc tgttcgaggt tgcgattggc cgactgcaaa gccgagcgca attgcaggat
    38221 ctgctcagtg accacctgat cgagcgtgtc acgatccagc aggtttagat ccatcaaaga
    38281 ctggccgagc agataatgat tgccttttgc gatctggtcc tgctgatacg taagcgcccg
    38341 ttgcaggtct gcttcggtga tcagcccttt ctggacgatg tactcgccca tacggggaat
    38401 cagcatttcg ggggtaagtt tcggaaggtt ttgtgacatg gggtttctcg gtaannnnnn
    38461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatcaat cttccagcac ccattcaccg
    38581 tttatcatcg ttgattttgc tttaattgtc aacagagatt gcgggtcggc ggagaagatg
    38641 ttttcttcga gcacgatcag gtcggcgagg tagccttgtg caagtttccc gaggcggttc
    38701 tctgcattgg cggcgtaggc ggcgccgacg gtgtaggcgg ataatgcttc agccaaagtc
    38761 aatttttgtt cggggtacca gccttcggcg gagggggatc cgtcggctct tcttcgagtg
    38821 actgcggcgt gcaatcccaa aaatggattt ggggattcga ccggggcatc cgatccaaat
    38881 gcaagggttg ctccatgatt cagttgtgta cgccaggcgt aggcatgtgc ggagcgatct
    38941 ccccagaagc ggtcggcggc gaacatatcg gagggtgcgt gaatgggctg catggatgcg
    39001 atgacgttga gttgggcaaa acgcggcgcg tcatcggggt gaatgacttg cacgtgttcg
    39061 atgcggtggc gcagatgcgg cagtcctttt tctgtttcgt aggcgcgcag ttgttgatag
    39121 gcgttgagca cttcgtgatt ggcgcggtca ccgatggcat ggacggtcat gctcaggccg
    39181 acatcggcgg ccttgcggca gtgctcgaac aattcctcgc cgtccatgtt gaggatgccg
    39241 cgattttcgg gttcatcctg atacggttgg aacatggcag cggtatgcgg accgagtgcg
    39301 ccatccataa aagctttgac agagccgatc cacagccagt catcgccgaa gccggtgcgc
    39361 aggccaaggg cattcgcatg gtccacactt tcgacgggga tgtttttgtt cacgcgcaat
    39421 tttagtttct tttgcgcatg taagtgttgc aaagccatga aagaatcacg gcggtcaaaa
    39481 tcatgaacgc ctgtgaggcc catcttccat aaaatggatt gggcgttctc aattgcagat
    39541 tcgatttcgg aaatggtcgg ttcgggtatg gtgctttcca ccaggctcat cgctgtttcg
    39601 agcaggatgc ccgttgcgcg gcctgtgttg tcgcgttgaa tggctccgtt tttggggtcg
    39661 ggggtatcgt tggtgatgct ggctcgctgc aatgcggcgg tgttcgccca gcccatgtgc
    39721 agtgattttg ctgtgagata aacggggttg ttcggggcaa tggcatcgag ttcggaggcg
    39781 gcagggaagg cgttgtcatc ggtccattca ttttgattcc agccgtgacc gaggatccac
    39841 gcgccgggtt tggctttttg ggcgcgctct gccacacggc gcagacattc tgccttggtc
    39901 tttgtttcgc aatctacctt ttgaagggaa agcccgtagt gttgaagatg caggtgcgca
    39961 tcggtgagtc ccggcaaaat ggttctgccg cccatatcct gtttttccac cctgccatgc
    40021 gcgatggctt caagcgtatc tttctcccct actgccacaa tgcgcccgcc ttcgatcaaa
    40081 atggcggagg cctgcgggtg cgatgaatcg agtgtgtgta tatttgcgtt gtagagaagt
    40141 ttcatgggat gtacctttaa tttttgattc tatcatacgc atcatgacaa aatttgcaga
    40201 gatcttttca gcaatcacct tccttttcct taattcggtc ttcacacacg tttcaccgaa
    40261 aagccatgtt cttcaagccc cgtgcctgtt tatgggcagg aaagccaatc cctactgcaa
    40321 aaacgataat aaatagaaag aatacatgca agcgattgtt gtatgacatt tttcctccat
    40381 gaatttctta agaacaaatg cctgtgggtg gggtttgtgg tataggtgtt ggaagtatca
    40441 ttcctcccgg tggttgagta acgtgatatt ttccactaga cactaccctt actgtgtctg
    40501 cactggtaat aatataaagc atctcggcgt ccccatagcg actggttggg gcatattgtg
    40561 agtaagtact aataaagaaa ccccattcac cttcataaag gatgcacaat tcgagttcgt
    40621 gggcttgagg ctggtttgca attagcgagc ctttccactc taaatcgcca ctcatcaact
    40681 tcaccccatc gggcaatgtg atgttgatgg tgacatcttt ctcgtcatta atggacataa
    40741 tctcgacgcg tagggtggcg gtttgcccaa tgacaggatc gggatgcacg gaaatatccg
    40801 ttacctctag cggtttactt tcgcaggcag ttagtgttgc cagaatcagc ccaataacaa
    40861 gcagggtttc caatgtacct tttgtgttgc gagttttttt catgatgtta ctcccttatt
    40921 tttttgattt tccgcatgct caagccgtca tcctattcgg gcactttcct cggcggcaga
    40981 aactattttg agattccaga acattccgaa gatacggtaa tggggcgtgg cgtaggacga
    41041 ttggcgtatt cttcctgact gaatgtatac tcactgcttg ggataagctt gcccgaatcc
    41101 aatgtgcttt ccaagtggat tatgtcaaag tcataccccc cctcctggtc ggatgaaggt
    41161 aaagcggtat aaagtcgtac gggccaggag ccttcttcca ttacacatat tttcacttga
    41221 aatattttgg gttgattggc agtaagtgtg ccttgccagg caggctcccc gcttacgagg
    41281 tgaattttat tgccagtatc ttccaaggtg tcaattgtaa attttacgtt ggccttatca
    41341 tctacagaca cgatctcaac ctctaaagtt acaacctccc ccacaattgg ctcaggaaag
    41401 actttaacag aagtaatatt gagtgggtac cctccgcttg aactgcaagc gctcactgtc
    41461 aacccaatga atagaaaaag acttgccatc cttttttgtg tattgatttt tttcatgatg
    41521 ttgctccctt attttttgat ttcaagaagt tcgtaatgac tcatcgaact tcctttttac
    41581 tttgtacgtt gtgggttttg aaaattgatt tacccgcctt gattttttgt ccaaacagat
    41641 tagaacaaaa aaaatacaac tatcaagctt tttgcaacaa tgacgagttt gttttttttg
    41701 ttgaattact ttttttggaa atgttctttt aaagtatcac cgttattttt gtaggggcac
    41761 ttttttattc ccccagaatc ttttcaaatt acaaaaaagg gacacagcgg cgctgtgtcc
    41821 ctacgggtta gatcaatttt tcgagaagcg catccagttc ttcgcc
//
LOCUS       DAOQJC010000019        39257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_434_length_39257_cov_9.137212, whole genome shotgun sequence.
ACCESSION   DAOQJC010000019 DAOQJC010000000
VERSION     DAOQJC010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 39257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39257
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_434_length_39257_cov_9.137212"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..494)
                     /locus_tag="PLE39_04435"
     CDS             complement(138..494)
                     /locus_tag="PLE39_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE67603.1"
                     /translation="MKTILVVEDTELNIDLITQLLEDDYNLLVARDGIEGVSMATEHT
                     PDLILMDISLPILDGYEATRTIRKTLPNLPIIGLSAHAMDGHEQKAKDAGCTDYLTKP
                     VNDDLLFKMLKKYLGE"
     gene            complement(514..3843)
                     /locus_tag="PLE39_04440"
     CDS             complement(514..3843)
                     /locus_tag="PLE39_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE67604.1"
                     /translation="MAKKASPVRKAVKKGAKMQATESGQRLKSHPPSAPVTDPSSPIT
                     LAHNLAWMGQHTQAIKLTSLELSKDEHSLATRIGLLNQRVESQLAQGRFDLAAKDADT
                     MMELTEADQGELKVQTLNCKTLVQIHSGDVQDAIKTATLALDIGSTNAIRNKRLLAMS
                     HFRMSEAYKNVSQFEAAVTEAQKAMEIFLAEGDLSGAGRASYIMAPSYNVLGFAQKSR
                     HAAQTALELCQQVNDQLGIGNALNGLSSTDPDLAERIHHYQQAIQAFEAVGHLERQST
                     STGNLGFVYAALGLYTHARRLTRKAFEIDKAIGAKIGMAYWSENLFDTELRMGLIESA
                     REHLNEMGRLAAEFGDPRLSASFNTNQGLMLLAEGKTQQAVQCFQDGVQTAREAEIGN
                     ENVHLSYLAIAHLKNNDPVAALAATTAATELHRSQDYKLPDSYSSHEIWWTHARALFA
                     NGKEVEARKALARAHEFLLEAIANVRDEGLRRNCLNKVKENRELLKFWISDNLNASGR
                     RKLSKKKIFAHQYLESNTREPFKRLTDTALRLNALHTTSEMHTFIVEEATELSGGERV
                     LFILEKNGVCEAAESILPRGENVQEVLNLIDPYLTQARLTRTVQLILPANDSDSTLSR
                     IIAPLIAQNQIIGYIYADMDSLYGTFTEVDRDMLGMLANQAAVALDNVNWAQGLELKV
                     QERTAELHARVDELAILNSVQAGLASQLDVNAIYELIGEQLRSLFDSQAISIISFDLK
                     KGTRHYHYMLEQGQRHDIPDAAIPPLSQFLIQNKRPILINENFKERLASIGITSQTVP
                     GTKPTKSLLRVPIMDGDEVRGVIGLDNIDRENAFNNADVRLLTTLASSMSVALEKARL
                     YQQAQEARAAADAASAAKSAFLANMSHELRTPLNAIIGFTRIVRKKSEGVLPEKQIEN
                     LDKVLTSGEHLLGLINAVLDIAKIEAGRMEVHAANFNINALIEQCYNIALPSMKPAVK
                     FDRRASEQLTLIHSDQEKIKQIMLHLLSNAAKFTHEGQVILAVEKVDASAEVPVPCFR
                     ISVSDTGIGISEEALGFVFGEFQQADNSTTRQYGGTGLGLTISRNLANLLGGELTATS
                     ELGKGSVFTLTLPIQFQPKKN"
     gene            4280..5221
                     /locus_tag="PLE39_04445"
     CDS             4280..5221
                     /locus_tag="PLE39_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67605.1"
                     /translation="MTTIFFATDIHGSDICWSKFLNAGKFYGADKLVLGGDMTGKAVV
                     PFVHQGGKNYRITLLEQVFETTNDEELADMTKRVRSRGYYPYLTNPDEIAELEKDPDR
                     VSKIFLQQVLKVVQQWMDLADKKLDGTDMMVYCSPGNDDMDEVDEIIRSSKRVMLVEG
                     LVSQLDENHEMIASGWSNITPWKTHREENEDQLAVRYEAMYSRLKDPRNSIFNIHVPP
                     HKSGLDEAPELDENLRPVLAGNSMIPVGSTALRKAIEKVQPLLSLHGHIHEGRGATRI
                     GKTLCINPGSMYEQGTLLGAIVKLGKNKIENYVLTTG"
     gene            5274..6920
                     /locus_tag="PLE39_04450"
     CDS             5274..6920
                     /locus_tag="PLE39_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNE67606.1"
                     /translation="MSNLFVRKATGMVRSWSVFDAFIYAFFSINFVTLGFYAFSQMYY
                     FGGGMINALIISAIFLVFEIIVYASLIAVMPRSGGDYVWMTRIFGGSIGFILAITGWW
                     FTLWLWTPVYGDMFRQVVLVPIMGVLGMRDTAVWFAGTGTPLFVSSLITLAFVSLVII
                     LGMKTYARIQKFSFYAGVLGLLIVIVLLFTGSPDKFKAGFEKNAAAIFGSQPGVYDAT
                     VQTGTDAGAATPLTGGSFSLIMVTLPWLVFFNLWPNWGATLYGEVRGATDYKRNFSGM
                     AWALGTVTVLGIVFFLGVAKTIGWDFYMQANGSWWNYYWGYVTDVPPLPVWPYPAMLA
                     TFLTESRALQLIVMVLMSFWWFGWAGTLFLSSTRVVFAAAFDRLLPEKVAELNERGTP
                     VNAMLLMVIPAVIVSYMYAYNVGGFISVTLAATQAIAIMYFGTAIAAIVLPYKKKDLF
                     EASPIAQMKVAGIPLITIAGVIFASFLGFLLVEWFFDPWLNTGGEGAGLYGISFKNTN
                     SVIFLLVTYGLAAAIYYGFKSYRKKSGIDMDKVQTEIPVE"
     gene            6996..7895
                     /locus_tag="PLE39_04455"
     CDS             6996..7895
                     /locus_tag="PLE39_04455"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HNE67607.1"
                     /translation="MPVIDEVVSKMEAWKGRDVSIEHLSGGLTNTNYKVHVDGRPFFI
                     RVPGEGTELLAIDRNNEYHNSRAAANAGVAPQILHHFPEYSVMVLEFLNGKTMSKDSL
                     NQKGMPARMAQSIKKLHAGERFLTDFNMFRLTEYYLRLCKERDIKIPDGYLDRLETVA
                     LIEKAMSVNQLATVPCNNDLLAENYIDDGNQLWLIDYEYSGNNDPTFELGNTCQEMQF
                     NDEQIREVCAAYFGDVSPAMIARMKLNMIMSDVGWGLWAAIQEKISTIEFDFWGWALE
                     RWGRAQEKMDSPDFDIWLKDVRQ"
     gene            7985..8725
                     /locus_tag="PLE39_04460"
     CDS             7985..8725
                     /locus_tag="PLE39_04460"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNE67608.1"
                     /translation="MDIIQEMHRLIDAANGKNIQLRAIGGLAVQSHNKKNHPLFLREF
                     ADLDFVVAKKQRREFEAFMPEVGYAPHRQFNVLNGDQRQIYYHGETDMKIDIFVGDFM
                     MCHKIPLENRLKADPVTIPLAELLLSKAQIVHLNRKDALDIASLLLNIEVGTGNDNII
                     NLAVIAQLCGSDWGLYKTTSINLERVKEVVSADDVALTSDEKSLIVSRVDKVLDAFAA
                     MPKTLAWQMRDKVGTRVRWYEEVEEVAR"
     gene            8722..8859
                     /locus_tag="PLE39_04465"
     CDS             8722..8859
                     /locus_tag="PLE39_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67609.1"
                     /translation="MNKHLRLFFAVLILTLSISLLVWGYMPNPHETRIQWIAPTEMQL
                     P"
     gene            8856..9602
                     /locus_tag="PLE39_04470"
     CDS             8856..9602
                     /locus_tag="PLE39_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67610.1"
                     /translation="MKEVLKPLYRLVVFPVFMVVFISLACVGNGNAPSSASEPTAISA
                     LATQTTNEVFPEPAPAIPETRRLTLEFPPKMRAGVEGDIVRLTLEVDDLGNITPTAQF
                     EGNVVTGEVIEIPDLYETHNVTAEARLDMAGMQIQPSGAIYEPLKRGQSATFFWSIRP
                     QDTGVYRGTVWLHLNFTDISTGEQSRIAVSAHTVEIEAVDFFGLSVNVARTTGVVGSV
                     VGGILGFPFLEDIIKFLLKFRRKKQSPSAN"
     gene            9708..10061
                     /gene="ndhC"
                     /locus_tag="PLE39_04475"
     CDS             9708..10061
                     /gene="ndhC"
                     /locus_tag="PLE39_04475"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNE67611.1"
                     /translation="MNEWLYVGLFFIVGLIIPVGAIGVGWLLSPKKPNPIKQSTYECG
                     IEPVGEGWVQFKAQYYIFALVFLVFDVEAVFLFPWAVKLNQLGLFAVVEGIIFILILV
                     AGLVYTWRKGMLEWA"
     gene            10184..11377
                     /locus_tag="PLE39_04480"
     CDS             10184..11377
                     /locus_tag="PLE39_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNE67612.1"
                     /translation="MNFWNDPLKIAADWLTGIFVGWGMNDTAAHVLVAFLGVLILISI
                     LMVVDIFLVWIERKVVARFQDRIGPNRLGPFGLIQPFADIIKLMIKEDTTPDGADKVV
                     YNLAPMLSMMSVLILWAVVPLAPVMLGVDLNAGALFIIAAGSIGTLSIIMAGWSSNNK
                     FSVIGAFRQVAVMVSFEIPMLATLLIPTIFAGSMGMNAIIQGQDIWFVLLSPLAALIF
                     LIAAIAELGRAPFDLAEGESELVSGYNIEYSGMKFGMFYAGELLHAFTFGGFWAIFFF
                     GGYRFFGLEKVSPFLAIAIILLKALVGYWIIMWVKYTVMRIRIDQMLAFNWKFLTPVA
                     FVLVMVTALMNALLREASSGLYITGMILSNIIVGWVSLEIVRSMSRKERERVEGVKQV
                     VEAHH"
     gene            11454..11963
                     /locus_tag="PLE39_04485"
     CDS             11454..11963
                     /locus_tag="PLE39_04485"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNE67613.1"
                     /translation="MTAEQIIFLIVAGVTLASALLVVTTRNLVHAALWLVATLFGVAI
                     IYAILNAGFIAVVQVVVYIGAIAILFIFAVMLTRRELRDNTPQLNRNWWSGALVAVLT
                     FAGLASLLSRWSGFSKTAAALPSGFDAVAELGNQLVSPDAFVLPFEVASVLLLAAMVG
                     AVYVAFTRK"
     gene            11976..12284
                     /gene="nuoK"
                     /locus_tag="PLE39_04490"
     CDS             11976..12284
                     /gene="nuoK"
                     /locus_tag="PLE39_04490"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNE67614.1"
                     /translation="MIPLSWYLIFAAALFSVGLFGALSRKNAVAILLGIELMLNAINV
                     NLVAFWRYGDVAVMAGQVFAVIVFAVAAAEVSVGLALVISVYRRRNTVIADELDMLKW
                     "
     gene            12294..14447
                     /gene="nuoL"
                     /locus_tag="PLE39_04495"
     CDS             12294..14447
                     /gene="nuoL"
                     /locus_tag="PLE39_04495"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNE67615.1"
                     /translation="MTHEFVTNLVWLIPLPPVIAFALIVLFTNKSKALSHTVAISAAF
                     LSWLGAMIVFWNAVKVEHFAEEPFAASVNWLPTGETWLKVGVLIDPLSATILFFVAWT
                     VLMIFLYSVGYHNFGQPAGDHDHKGLPPHGATVTDDLGHKHTVLSIEPMYSRFFAFIS
                     LFAFGMLTLVVSDNILTLFVGWEIMGLCSYLLIGFWYGKESARNAAIKAFMTTRIGDV
                     FMLLGISFLYSVTGTLSFREIFTEEILHTLASVPSGVFGLSAAGLIGLLLFIGTVGKS
                     AQFPLHVWLPDAMEGPTPVSAMIHAATMVSAGVYAVIRMFPIISLDEKTMAVVAFIGA
                     FTALFAATIAVAQNDIKRVLAYSTISQLGFMIAALGIHAYVAAVFHLVTHAFFKALLF
                     LGSGSVIHGMEHGVMHTGNHKVDPQDMFNMGGLRKKMPITFWTFLIGGFALSGFPIVT
                     AGFWSKDEILADAFGHGHTVIFGTLAFAAFLTAFYTMRQITLTFLGEPRTKEAEHAHE
                     TPWTMTTPLVVLSVFAVTYGWVGIPEHFPLLGGLVPNWFHEFVGSTLHEHPEALEFSI
                     VPLLTSLGVALGGLGLGWLVYRNVNSPAEDRLQIGLLKNKYYFDEIYDVVFVKPAYWL
                     AENVIYKFMDQGVIDGILHFFGPATGGVGSTIRNYFDIPVINHLIGDGSADATYWIGK
                     NLRPIQTGRIQQYLILSLILLFVVGGLVYYILTLA"
     gene            14457..15980
                     /locus_tag="PLE39_04500"
     CDS             14457..15980
                     /locus_tag="PLE39_04500"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNE67616.1"
                     /translation="MDFLNSHLLSLILFLPTVAAVIMLFLPSEEAKLQRWFAFTASLI
                     PFGLTLYLWNAFDPNRTGFQFQEQYTWYEAIGSNFHLGVDGISLSMVLLTTLLTPLAI
                     LASFSVSDKVKAYFMLFLFLETGMLGVFMALDLLIFFVFWEIGLVPMYFLINQWGSAN
                     RNYASLKFMIYTMAGSLGLLLAVQMLGVLFGTYDLVTLYERWNSLDAGSVLLGMPVHT
                     VKTIAFWAFVVAFAIKVPVWPFHTWLPDAHTEAPTAGSMILAGVLLKLGAYGFIRLVL
                     PFYPLEAKIFAGALAFLAVAAIVFGAFSSYAQTDFKRLVAYSSVNHMGFVMLGIAAAG
                     LASGTQDAHIAMSGAVLQMFNHGLSAAGMFFLVGVIYERTHTRNLNDFGGLFPLVPVY
                     GGILIFTSMASLGLPGLNGFVSEFMVVRGAYPVLTTYTAISMLGLLFTGAYILKGIKS
                     VLHGPLNEHWAHGEHKLTEINTREILVMVPLMVLILVIGVWPMWLLDVINKAVTALF"
     gene            15991..17508
                     /locus_tag="PLE39_04505"
     CDS             15991..17508
                     /locus_tag="PLE39_04505"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNE67617.1"
                     /translation="MTNFPILSVITFLPILAAAIILMMPANRKNEIRAVALAAATLDL
                     LLSGWLYLQYLLGDMTGYQFIEQYNWLPALGISLKFGVDGMSTPLVLLTGIVMFTGVL
                     ISWGDEDPHVKAGIQDRPREFFAFLFLLAGGVFGVFVSLDLFMLFFFYEIAVFPMYLL
                     IAIWGWVKTREYAAMKLTLYLFIGSVVALVGALAMYWTQYQNTGVLSFDMLLMENAKF
                     SPTFQYVWFLPVFLGFAVLGGIWPFHNWSPDGHVAAPTAVSMFHAGVLMKLGAFAALR
                     VGVMLLPEGAKDWAWLLMLFAGVAVVYGAFIAFVQTDMKYMIGFSSVSHMGLVVLGVS
                     TLNHDGLVGAGVQMFSHGVMTALFFAVTGMIYDRAHTRMIPELGGIAKVMPFATVGFI
                     IGGLVSMGMPGFSGFVAEFPIFMGVWGRQWLVAVIASISIVITAAYIMINIRKVFFSE
                     MPENLHGHIGDVTMLDKIAITTLCLFMIVIGMFPAVMVPMVEKGVENVLRLLGGA"
     gene            17508..18956
                     /locus_tag="PLE39_04510"
     CDS             17508..18956
                     /locus_tag="PLE39_04510"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNE67618.1"
                     /translation="MFTSTTFASILPETLILILGIILLIVEPFWKAENRRNVGWLTAV
                     GLFIAMLVSLLFGQPGEPTSVLGGMIRFDWLGFFFKMLFMFAGAATALLLMDHEKAGL
                     RGEAYLMLLASIIGMNLMAVSSDLVMMYLAIETASIPLYILSGFLLGDERSTEAGFKY
                     LLFGTLASTVMLYGFSLIFGFSGTTNIYKLSEMLAVGELPLFVNFGLIGLVVVGLGFK
                     IAAVPLHFWAPDVYQGAPTPVAGFLSTASKAAGFAVIIRLFFVAFPEIASYWTVILAA
                     LAAVTMTAGNLLALSQTNIKRLLAYSSVGHAGYALIGVVAFSQLGAASVVFYLAAYIA
                     TNLLAFGIVMAFSRVAGLEDITDYAGLSRRNPLMGLMMLAAFLSLAGMPPFGGFVAKV
                     FVFAAGVQANYTWLVVVGILNSIIGVYYYLNVLKYVYLYRMPNEDEENHPIPLTRPYT
                     IALVVLALGVILVGTVFAPWFSWSDAAALNLF"
     gene            19200..19478
                     /locus_tag="PLE39_04515"
     CDS             19200..19478
                     /locus_tag="PLE39_04515"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNE67619.1"
                     /translation="MTQSKQTNRQNMVYTLLVVMVGQVGCLTIAVILASVFGGLWLDN
                     TFGTKPVFTLVLLFAGIPLSVFLMIFAARKTLARLKEKYETEEQKTTA"
     gene            19488..20492
                     /gene="atpB"
                     /locus_tag="PLE39_04520"
     CDS             19488..20492
                     /gene="atpB"
                     /locus_tag="PLE39_04520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNE67620.1"
                     /translation="METQKRTPWRRWIVLVLMIVGFILGGIFVPVQPEITVAAEKLIE
                     EPLIENFLGQPFYLVNTLPTLFVTVFIILLPLAWSAGRSLKKSQETDLVPRGVGNTME
                     AILEMLYNMTESSAGVKWAKTIFPWFATIMIYVLVANLLKLLPGFESIGVIHHAHGEG
                     HLTQELGNFLGFRWELLSPDKVEEGGYILAPFFRGISVDLNFTLSLALISFFAIQIIG
                     VRSQGIGYFSKFFNTKRMFKVPFFGAMDFLVGLLEIISELAKILSFTFRLFGNMFAGI
                     VLVAVVASLLGKISILPAMIMMFELFVGLIQAFVFGMLTMVFMAMATQGHGDEEHAEH
                     "
     gene            20546..20785
                     /gene="atpE"
                     /locus_tag="PLE39_04525"
     CDS             20546..20785
                     /gene="atpE"
                     /locus_tag="PLE39_04525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNE67621.1"
                     /translation="MEGNILEAMRFVGAGLAMIGAAGAGVGIGLSVQGALTGMARNPD
                     TYGNLLTNMILGVAFSESIAIYCLVIAFLMLFKVV"
     gene            20804..21523
                     /gene="atpF"
                     /locus_tag="PLE39_04530"
     CDS             20804..21523
                     /gene="atpF"
                     /locus_tag="PLE39_04530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNE67622.1"
                     /translation="MEALGINLGLLIVQLIAFAIVFLTLNAWVYQPMLDMMESRKQKI
                     SQGLEDARVAAEARANAEKEAAKVIAEAQAEASKVVREATERAAAAGKDVKAAAEAEA
                     AKARETAMAEAELERTRILGDLRGQVSALAIAAANKLVGEALDEKKQHALIDEFFSGV
                     KAGKVVVVDGEFKGDAAEVTSALPLNNQEQDAVKKSFGAKEVSFKVNPAILGGLVVKI
                     GDKVLDGSVAGKLEGLRQTLK"
     gene            21599..23170
                     /locus_tag="PLE39_04535"
     CDS             21599..23170
                     /locus_tag="PLE39_04535"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNE67623.1"
                     /translation="MLLKKLFVDFPLSMPEVIPDVEISGIAIDSRAVKPGHLFVAMKG
                     GFADGHAYIQKAIENGASAVVGERELDGLSVPYLRLENPRQALTWLAAAFYNWPARKL
                     TVIGVTGTDGKTTTSNLLHKILLAAGIKAGMISTVNAVIGDEVLDTGFHVTTPDAHDV
                     QAYLARMVEAGLTHVVLETTSHGWSQHRVDACEFDIGVVTNITHEHMDEHGSYENYRA
                     AKARLFSSLEWTRSKPAGNPRLAVINRDDSKSFGFLDEFIKVNKVNYGLGKDADVCGM
                     DIEYNPAGIRFVATSKDFRVAASSRLVGAYNVSNCLAALSAAVYGLGIKPEVAAQGIA
                     ALEGIPGRMERIDLGQNFTAIVDFAHTPNALKVSLEAGRTMTRGRVISIFGSAGLRDK
                     EKRRMMAETSAELADLTILTAEDPRTESLDGILEEMAAGAIGKGGREGETFWRVPDRG
                     EAIKFALRLAREGDIVLSCGKGHEQSMCFGKTEYLWDDRTAMRAALADFLGVDGPAMP
                     YLPSRDMNESDWLKG"
     gene            23190..23651
                     /locus_tag="PLE39_04540"
     CDS             23190..23651
                     /locus_tag="PLE39_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE67624.1"
                     /translation="MIHTLHREQIIPTSVEVLWDYFSAPQNLNDMTPPDMNFQIVMGG
                     DVKMYEGQIIEYRVEFIRGIRSLWLTEIAHVREKEYFVDEQRVGPYRFWYHEHKFEPV
                     AGGVKMTDHVTYAAPFGFLGDLVNAVWIRRRLDAIFDFRHRRIIELFGSVK"
     gene            23648..24244
                     /locus_tag="PLE39_04545"
     CDS             23648..24244
                     /locus_tag="PLE39_04545"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNE67625.1"
                     /translation="MTKPWATPVVLQGKYVRLEPLSEAHVDGLAEIGVGQDFWDFMLY
                     GRMETKEDMRNWVKDIMGRGEKGTDLPFTVIHLDSGRVAGATRYLNIMPNDRGLEVGG
                     TWYGLDFQRTPVNTECKYLLLSHAFEVLKCIRVQIKTDSRNIRSQKAIERIGAVKEGV
                     LRNHMILPSGIYRHSVFYSIIDTEWEQVKKRLEAFLEG"
     gene            complement(24335..25789)
                     /locus_tag="PLE39_04550"
     CDS             complement(24335..25789)
                     /locus_tag="PLE39_04550"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNE67626.1"
                     /translation="MNLPKLIQGGMGVAISDWRLAKAVSNLGQLGVVSGTGISRVLAS
                     RLMDGDISGNVRRALSNFSLPEPVQQIIDRYYTPGGKSPQTPYKTPAAYTIKPPKFLD
                     QVTAIANYVEVFLAKEGHNGLVGINLLEKVQMPTLASLYGAMLAGVDVVLMGAGIPTQ
                     IAGILDKLSNHEPVSYRLDVHGAGKDDDHRIHFDPEAIFPGIAKLVGKLKRPKFLPII
                     SSVVLAQALIKRSEGSVDGFVIEGPTAGGHNAPPRGTVNYNEKGEPIYGEKDAVDLEK
                     MKALGLPFWLAGGYGNPKQVQSALEAGAAGVQVGTAFSLCEESGMRPDLKQRLLEKVR
                     AEEAQVFTSPLVSPTGFPFKVAQLEGTLSDPEAYSARNRICDLGFLRILYKREDGSVG
                     YRCASEPVDVFVKKGGNLEDTVNRSCLCNNLTATAGFPQVRKDGYVEEPIITSGDDLV
                     AVGQFLKEGKTSYTAEDVVRYLLGPFMNVGQVTA"
     gene            complement(25884..26303)
                     /locus_tag="PLE39_04555"
     CDS             complement(25884..26303)
                     /locus_tag="PLE39_04555"
                     /inference="COORDINATES: protein motif:HMM:NF026937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm63 family immunity protein"
                     /protein_id="HNE67627.1"
                     /translation="MFEPSFEKIQAELREYCRRMDPAYAHVKIQTIRGDDGTSHLEIR
                     DNSYHYIATERGLILSEKQTSDKNELLYWLVDEIAWSKACAYEFKHRVKGQSFRRLLF
                     AKNIEYLMMVKPAWAERKQKEFEAILAKHPYDDAAEG"
     gene            complement(26331..27584)
                     /locus_tag="PLE39_04560"
     CDS             complement(26331..27584)
                     /locus_tag="PLE39_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE67628.1"
                     /translation="MASKLKILFNQYPRQFWLMVAGIVISTAGASMIWPFLLIYAGGK
                     LNLPLSTVATLISINAGTGLLASFLAGTLTDKIGRKAVMVFSLVVNGIAYLFLMQAET
                     YPQFIALMIFIGLCNPLYQVGADAMMADIIPTEQRTDAFAINRIAHNAAFALGPAIGG
                     FLAASSYDLAFYGAAVGFFAHSLLMFLFAHETLHKTVGATNGHSLADQPSVMEGFVRV
                     FRDGDFMAFVVLTGIGLIAPTMVWILMPVYAKTNFSLSEALYGWIPTTNAVMCVFIQY
                     AVTRITRHYKTLPVAAAGMFIYAISVGSVALMSGFWGFWLSMVMLTFGELTLVPTASK
                     YVADTAPADLRGRYMSVYWLAWGLARTLSPLIGGFINDSISPRAIWIGGLLIGMTSTA
                     GLLILNKRTLAQAALRPQPKPDSAS"
     gene            complement(27699..29393)
                     /locus_tag="PLE39_04565"
     CDS             complement(27699..29393)
                     /locus_tag="PLE39_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67629.1"
                     /translation="MNDLKDKPSFFSNPRLRITALVIILAALLLYYIDVFRTAGPTTF
                     DDAYMFIRYADNFIHGYGFAWNPDGVQTYGATSILYLGLVIIARIIFQGLGSGKLLVA
                     LSAGLGLPAIVLTAYTVSRYAESKLLKSSFLWLTVLLVGYLVTAPVYLFHMKSGMDST
                     LSFLCNALLIFSTLGWVYNENRYRLFSTLTVIAGYLAFLARPDNLLYTFFFPVLAILF
                     LAPEGRRKRLVHFLGWLGVLLALDTLVKFIVFSDPLPLPFYAKSSGFYEGYIGTHTWN
                     PIEYLFAFGTLALPFLVLIVLSSTKQTLKLLAVFLLPIALTFTYYFTVVQIMGFEARY
                     YFPALPYLIVLSILMLDRSLSSGDTEGESHGKRLMRVLLLLLIVALFSNSTLKTITSD
                     AYKNSQIAATKLYSPHTEYTTPAAKELPERGTWPMVLAVAKFSKSLPEGIIFAQSEYG
                     FTGAESPHLYIVDIIGLHDSYFAHNGFSVDEFLRRKPDLIWFPHFDYTKITAEIIDSQ
                     TFWEQYEFYPGAFDFGLAIRKDSLNYQAIHKAIEPVWREVYETRQMEDYLATPISK"
     gene            complement(29507..31765)
                     /locus_tag="PLE39_04570"
     CDS             complement(29507..31765)
                     /locus_tag="PLE39_04570"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNE67630.1"
                     /translation="MKPESKRYSAVVGTRTFTFETGKLAAQAGGAVTFGVEDSIVFAA
                     ATMGGVRDGIDFFPLSVDFEERMYAGGKIPGSFFRREGRAATDAILTGRLTDRPLRPL
                     FAHGMRNEVQVIMYTFSSDGVNPLDILAINAASAAIMISDIPWGGPVGAVRVGRINGE
                     FVLNPTFDEMNASDLDLRIAGTKDAILMVECGANEIPEDVMVEALELGHKSIQPIIDL
                     QLQMQAEIGKPKREATIVTANEDLQKKVLDAVNARMNELLDKPLSKAEFYGGMDELQT
                     AIIAEFCVAGDENAPSEKDVKSAFSEAEHKIVRERILSMGKRPDGRTPTEIRPIWCEV
                     GLFPRAHGTGLFTRGETQVLSFATLGTLGEAQELDNLSPEKSKRYMHHYSFPPFSTGE
                     VKPLRGQSRREVGHGALAERALEPVIPSEETFPYAIRVVSEALSSNGSTSMGSVCGST
                     LALMDAGVPIKAPVSGVAMGLITDETGRYKILTDIQGTEDHLGDMDFKVAGTAQGITA
                     LQMDIKISGLSAQMMKEALAQANTARMSIMEKMLAVLDAPRAELKPHAPRIITVKIPV
                     DKIGALIGPGGKNIRALQEETGTKIDIEEDGTVYIASTDSVGAKIAQERVEGLGESVI
                     VGNIYTGKVVRIEAFGAFVNLLPGVDGLVHISQLASERVNTVEDVCVLGDELTVMVTD
                     IDPQGKIRLSRQAVLEGWSAEEAREKDKGGRGGGGGGRGGDRGGRGGDRGGRGGDRGG
                     DRRGGGDRGGRR"
     gene            complement(31942..32214)
                     /gene="rpsO"
                     /locus_tag="PLE39_04575"
     CDS             complement(31942..32214)
                     /gene="rpsO"
                     /locus_tag="PLE39_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNE67631.1"
                     /translation="MPLAKEVKTKAIEDFHRHDKDTGSPEVQIAILTNRINQLTEHLR
                     SNKQDQSCRRGLLKLVGQRRRLLTYLRSSSYQSYLSVTDRLQLRRK"
     gene            complement(32404..33186)
                     /locus_tag="PLE39_04580"
     CDS             complement(32404..33186)
                     /locus_tag="PLE39_04580"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNE67632.1"
                     /translation="MKELKFLFAIWKTNLQSAMEYRAAFLTQAIGMMVNNFIYFAIWI
                     IFFDRFKDVRGWGVNDMYVTFGVLASAFGLVSLFFGNAFNLSDIIAKGRLDYYLSLPR
                     PVLLHTIASRTIASGMGDFLYGFVSYGLSGYFTWDGLLRYVLAMLLAATVFAAFLILT
                     QSLAFWFGMMTNFSNLVLNAMLTFGIYPITLFDSYAKLILFTLIPAALMGAIPAEFIR
                     AFTWQTLGELLLGAIGFLTLAVFTFRMGLRRYESGSAIQIEV"
     gene            complement(33228..34028)
                     /locus_tag="PLE39_04585"
     CDS             complement(33228..34028)
                     /locus_tag="PLE39_04585"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNE67633.1"
                     /translation="MKSISKYWSIFQITLLNSLAYPGELVGRSLMIIPFMWIFYQLWK
                     VTFSAAGAVDGMINGLTLRDTLWYLMLAETIELGRPPIARTMSENVKDGSIAYILNKP
                     YDFLLYQFSTAMGETIFRAMLNAVFGGLTIWWLVGTPNHPEGFLIALPAILGAWVLHF
                     AINAMIGLSAFVVEDVSAFTWIYQKLAFIFGGMLIPLDFYPQWLQNLAKLLPFSSAIY
                     GPARLFVTPTYDLFVSVMTLQVTWIVALVVALTIVYRRGLTQLTVNGG"
     gene            complement(34113..34937)
                     /locus_tag="PLE39_04590"
     CDS             complement(34113..34937)
                     /locus_tag="PLE39_04590"
                     /inference="COORDINATES: protein motif:HMM:NF022431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HNE67634.1"
                     /translation="MDKNFWHTIKENKYAFPQGHDLTALTDELFSYIPSTDPDLRDGI
                     GYEIFANWIETEPYQAEQLRGYLKRLLENLQVGLGQQDDDSVFGRAFSVLYLAEVIHR
                     DNQKPYLESHEVKETLTRVLAYLAAERDPRGYVPVKGWAHALAHTADMLMVFARSPHL
                     GATELEHILSAVSEKMRAAKNWIYIHGEDDRLARAIVTALAREELSLEQVKAWFENLT
                     TDWKGAWDHEEQSRAYLNARNLLRAIHLRILQMKELPRQEELSALALEAAASMRPF"
     gene            complement(34952..35941)
                     /locus_tag="PLE39_04595"
     CDS             complement(34952..35941)
                     /locus_tag="PLE39_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE67635.1"
                     /translation="MLSIDVSNLQKTFQTKRKAAGLSGSIRSLFKPEYSLVEAVHNLS
                     FQMGTGELLGFIGPNGAGKSTTIKMLTGILHPSGGEAKVLGFTPWVDRQKLAYHIGTV
                     FGQRPQLWYHLPAIDTFMLFGKIYELDDREIKKRIDFLTEAFEIKDLLDVPVRKLSLG
                     QRMRCEVAASLLHRPKLLLLDEPSIGLDVVAKQHIRDAIKRMNQEEGVGVLLTSHDAG
                     DLEALCKRVIIVNHGQIVYEDKVSNLKRKFLTTKLVEVRYALEVSKDFAIQGVETLKV
                     GHYGVKLKFDTTQTPVDTVISHLSSAGDIVDITISDPPLEEVISKIYQEAGNA"
     gene            36000..36653
                     /locus_tag="PLE39_04600"
     CDS             36000..36653
                     /locus_tag="PLE39_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67636.1"
                     /translation="MRRLSWVIVLAFLLQACAGFSLNPPTSTPTITSTPTITYTPTIT
                     PTITITPTRTALPTIVRIPTWDPNQPTPTFMAIPIFIGSVTATPIVSPTPTRPGIGFV
                     SVDVSEQKIFWGSCKHNKAKIIAKVQNPDEVFSVVIFVRVKALLKEDYTPWTTGDVMT
                     KHGDGTFSYTLVGSEIEGHNHYRDSWVYFQLVATNEEGEIVGRTMIYTNSLSLSPCK"
     gene            36669..37367
                     /locus_tag="PLE39_04605"
     CDS             36669..37367
                     /locus_tag="PLE39_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67637.1"
                     /translation="MKKFYALSLCTIMLLSCSLNLLAPSISSTPVPTLTNTPTDLPSA
                     TPTVPTPTFTPTPPLVGLRTHTLTPTITATSTQPTETSIFLATLDTPTPSVEMKGFVS
                     VNVSTKEFFKDAECQPTSVKFVAQVADSAGTAFVVLFVRFKSKQTGTTSEWTSIGMST
                     IGAGTYTYELKPEEMKGVDSFKNPWVQYQFVATNADANEIGRTGIFSERLSLLECEPT
                     PSPSPSPEPTVLRP"
     gene            37364..38101
                     /locus_tag="PLE39_04610"
     CDS             37364..38101
                     /locus_tag="PLE39_04610"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNE67638.1"
                     /translation="MMEFPDDLIRFLTKATRVSVLTGAGASQESGLRTFRDAQSGLWA
                     QYKPQDLASPEAFARDPKLVWDWYAWRREAIKGVRPNAGHYALVDMEKKVEVFTLITQ
                     NVDGLHRMAGSSNVLELHGNILKVRCSECRTFTEVWGDDSESVPLCDKCGGMLRPDVV
                     WFGEALPREELEAAVNAARSCQVFFSIGTSGVVQPAAALAHAARNKGAVVVEVNAEPT
                     PLTPKTDFAFHGKSGEILPELVRAVWG"
     gene            complement(38117..38407)
                     /locus_tag="PLE39_04615"
     CDS             complement(38117..38407)
                     /locus_tag="PLE39_04615"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HNE67639.1"
                     /translation="MTIKFKGKIFHWRGPAPFLFVAVPEKPSRDLKAISSSVTYGWGV
                     IPVQVRIGNTEWKTSLFPKDGRYLVPIRMSVQKSENIGVDDNVTIYLEVAWP"
     gene            complement(38664..>39257)
                     /locus_tag="PLE39_04620"
     CDS             complement(38664..>39257)
                     /locus_tag="PLE39_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67640.1"
                     /translation="MGSEGVLVKTDDGPWESVEIYGLGPIKYKDANLIDAIGIVGPEI
                     FTISMVSIFLLLTNIIVNAKRTDRKEYIWLISSVMALVISVGLVIFLFYIQLFFTLTM
                     VLIRLSGFVAVVSIAFIIASSFVQLKNNLSKQGMNGMIALAFLWFISCGLYILWVYDV
                     IHETRPTYIAVIILNASYLVWLLWRIAARIEQYRTKI"
BASE COUNT         8658 a         9469 c        10667 g        10463 t
ORIGIN      
        1 cctctgtcgc tgaatcaatt gccgccgcgt gttgacccgt ccacgccagt tggcggatgt
       61 aagtcagggc ctggtcatct gtattttttg ttcttttgtt ttttgtgatg tctgattttt
      121 tcttcaccat aaaaagccta ttcacccagg tattttttta acatcttgaa caacaaatca
      181 tcattgacag gcttggtcag gtaatcggtg cagcctgcat cttttgcttt ttgttcgtgg
      241 ccgtccatgg catgagccga aaggccgata atgggtaaat tgggcagggt cttgcggatg
      301 gtgcgtgtgg cttcgtaccc gtccagaatg ggcagggaga tatccatgag gatcaggtcg
      361 ggggtgtgtt cggtggccat tgaaacgccc tcgataccgt ctctggcgac gagcaggtta
      421 tagtcatctt cgagtaattg agtgatgagg tcaatgttca attcggtgtc ttccacaaca
      481 agaatcgttt tcataaaagg tctcctaata acgtcagttt ttcttgggtt gaaattgaat
      541 gggcagggtg agcgtaaaaa cggatccctt gccgagctcg ctggttgcgg tcaattctcc
      601 gcccagcagg tttgcaaggt tgcggctgat ggtgaggccg agacccgtgc cgccatattg
      661 acgggtggtg ctgttatcgg cctgttggaa ttctccgaac acaaatccca gcgcttcttc
      721 gctgatgccg atgccagtgt cactgaccga gatgcggaag cagggtacgg gaacttccgc
      781 tgaggcatcc actttttcga cggccagaat gacctgtcct tcgtgtgtga actttgcggc
      841 attgctgagc aaatggagca tgatctgttt gatcttttcc tgatcagagt ggatgagggt
      901 gagttgttcg ctggcgcgcc tgtcgaattt cacggcaggt ttcatggaag gcagggcaat
      961 gttgtagcat tgttcgatga gtgcgttgat gttgaagttg gcggcatgca cctccatgcg
     1021 gcctgcttcg atcttggcga tatcgagcac ggcattgatc aaccccagca gatgttctcc
     1081 gctggtgaga accttatcga ggttttcgat ctgtttttcg gggagcaccc cctcggattt
     1141 tttccgtaca atgcgggtga atccgataat ggcattgagc ggggtgcgca gttcgtggct
     1201 catgttggcg aggaaggcac tcttggcggc gctggcggcg tcggcggcgg cgcgggcttc
     1261 ctgtgcctgt tgatacagac gggctttttc aagcgccacg ctcatgctgc tcgccagtgt
     1321 ggtcagcaag cgcacatcgg cattgttgaa ggcgttttcg cggtcaatgt tatcgaggcc
     1381 gatcacgcca cgcacttcgt cgccatccat gatcggtacg cgcaggagcg atttggtcgg
     1441 ttttgtgcct ggcacggttt gtgatgtaat gccgatggag gccagccgtt ccttgaagtt
     1501 ttcgttgatc aggatcggcc gtttgttttg aatcagaaat tgtgaaagcg ggggaattgc
     1561 ggcatcgggg atgtcgtggc gttgtccttg ttcgagcatg tagtgataat ggcgcgtacc
     1621 ttttttcaaa tcgaacgaga tgatggaaat ggcctgcgaa tcgaacaggc tgcgcaattg
     1681 ctctccaatc aactcgtaaa tggcgttgac gtccagttga gatgccagcc cggcctgcac
     1741 actgttgagg atggcgagtt catccacgcg ggcatgaagt tcagcggttc tttcctggac
     1801 cttgagctcg agtccttgtg cccaattcac attatccaag gcgacggcgg cttgattcgc
     1861 caacatgccg agcatgtcac ggtccacttc ggtgaaggtg ccgtagagtg aatccatgtc
     1921 ggcatagatg tagccgatga tctggttttg cgcgatgagc ggggcgatga tcctgctcaa
     1981 agtgctgtca ctgtcgttcg caggcagaat cagttgcacg gtccgcgtga ggcgcgcctg
     2041 ggtgaggtag gggtcgatca ggttgagcac ttcctgcaca ttctctccgc gcggaagaat
     2101 cgactcggca gcttcgcaaa ccccgttctt ttcgaggata aacagcaccc gctctccgcc
     2161 gctcaactcg gtggcttcct ccacaatgaa ggtgtgcatt tcacttgtgg tgtgcagggc
     2221 gttcaatcgc agggcggtat cggtcagccg tttgaaaggc tcgcgtgtgt tcgattcgag
     2281 atactgatgg gcgaagatct ttttcttcga aagtttacgc ctgccgcttg cattcagatt
     2341 gtcgctgatc caaaacttca ggagctcgcg attctctttg accttgttga ggcagttgcg
     2401 gcgcagtcct tcatcacgca cgtttgcgat ggcctcaagt aaaaattcat gcgcccgtgc
     2461 cagagccttg cgtgcttcaa cctctttgcc gttggcgaat aatgcccggg catgggtcca
     2521 ccagatttcg tgagaggagt agctgtcggg tagtttgtaa tcctgactgc ggtggagttc
     2581 agtggctgcg gtggtggcgg caagggcggc aacagggtcg ttgtttttca ggtgtgcaat
     2641 tgcaaggtag ctgagatgga cattttcatt cccgatctcg gcttcacgtg cggtttgtac
     2701 tccatcttga aaacattgaa cggcttgttg ggtttttcct tctgccagca gcatgagacc
     2761 ctgatttgtg ttgaaactgg cgctcaaacg cgggtcacca aattcagcag ccagcctgcc
     2821 catttcgttt aaatgttcgc gggctgattc gatcagtccc attctcaatt cagtgtcaaa
     2881 caggttttca gaccagtacg ccatgccgat cttggcaccg atcgccttgt ctatttcaaa
     2941 ggctttgcgg gtcaggcggc gggcatgtgt ataaaggcca agggctgcgt acacaaaacc
     3001 caaattcccg gtagaggtgg attgacgttc cagatggccc actgcttcaa aagcctgaat
     3061 ggcctgctga tagtgatgaa tacgctcggc gagatcgggg tcggtggaac ttaatccatt
     3121 caaggcgttg ccaattccaa gctggtcgtt gacctgctgg cataactcca gtgctgtttg
     3181 agcggcatga cgggattttt gagcaaaccc cagaacatta taggagggtg ccatgatgta
     3241 actggcacgg cctgccccgg agagatcgcc ttcagccaga aatatttcca tggctttttg
     3301 cgcttcggta actgccgcct caaattggct gacattttta taggcctcgc tcattcggaa
     3361 gtggctcatt gccagcaggc gcttgttgcg gattgcattt gtcgaaccaa tatccagagc
     3421 cagtgtggct gttttgatgg cgtcctgcac atcgcctgag tggatctgaa cgagggtttt
     3481 acagttgagc gtctgtacct tcaattcccc ctggtcggct tcggtcaact ccatcatggt
     3541 atctgcatct ttggcagcca ggtcaaacct gccttgcgca agctgcgatt cgacgcgttg
     3601 attgagcaga ccaatcctgg ttgcaagtga atgttcgtct ttgcttaatt caagtgaagt
     3661 gagtttgatc gcctgggtat gttgccccat ccaggcaagg ttatgggcga gtgtgatggg
     3721 tgaggagggg tcggtaacag gggcggatgg aggatgactt ttcagccttt gcccactttc
     3781 agtcgcctgc attttcgccc cctttttaac agcctttctt acgggtgatg ctttttttgc
     3841 catgcccgtt tctcctcaac ctctggtttg attaaatgga agtttatcgg gcggagcacc
     3901 ttaagtcaat gggagaagtt gggttggata tgtctgcaat gtgaatttaa tccgatctta
     3961 accaaccgac aactggttct taaatcccga ctcttatact acccgatcat gacatttcaa
     4021 aaccgtgttc tctggcttat gtatattctt ctggtccttg cacaatgggt gtgggctttg
     4081 tttctacggc gaagggagcg ggttgagtat ctgaaatatc ttttacaaga ttctcttgca
     4141 taattccctg gtcacctgat tgaatcattc ttcacaagtc tgctgcaaaa cggttgcatt
     4201 atattttgcg gggagtataa tgggcgaaat ctattccgca ccacctctgc aagttatccc
     4261 atttttcaag gatttgcatg tgactaccat attttttgct acagatatac atggttcgga
     4321 tatttgctgg agcaagtttc ttaatgcggg gaaattttac ggcgccgata aactggtact
     4381 cggcggcgat atgacgggca aggcggttgt cccgtttgtg catcagggtg gcaaaaatta
     4441 ccgcatcact ttgcttgaac aggtgtttga aacaacaaat gatgaagagc ttgcggatat
     4501 gaccaaacgt gtccgtagtc gcggctatta tccatatctg accaaccccg atgaaattgc
     4561 ggaattggaa aaagaccctg accgtgtcag caagatcttt ttgcagcagg tactcaaggt
     4621 tgtgcaacaa tggatggacc ttgccgacaa aaagttggat ggcaccgaca tgatggtcta
     4681 ttgctcgcct ggcaatgatg acatggacga agtggacgag attatccgtt caagcaaacg
     4741 ggtgatgctg gtcgaaggtc tggtctccca gttggatgaa aaccacgaga tgatcgcctc
     4801 gggctggagc aatatcacac cctggaagac gcaccgtgag gagaacgagg accagctggc
     4861 agtcaggtat gaagcgatgt actcgaggct caaagacccg cgcaattcca tcttcaatat
     4921 ccacgtgccg cctcataaat caggtctgga tgaagcgcct gaactggacg aaaatctgcg
     4981 ccctgtgctg gctggaaact caatgatccc cgtcggatcc actgctttgc gaaaggccat
     5041 agaaaaggtt caaccgttat tgagtttgca tggtcacatc catgagggcc gcggcgcaac
     5101 aaggatcggc aagacacttt gcatcaatcc aggttccatg tatgagcagg gaaccctgct
     5161 tggcgcaatt gtcaagttag gaaagaacaa gatcgagaat tacgttctga caacaggtta
     5221 accgtaccgc aaaatttatt tgcagtacta ataaaaccaa agaggagaga gttatgagca
     5281 acttgtttgt ccgtaaggcg actggcatgg ttcggtcctg gtcagtcttc gacgcattta
     5341 tttatgcgtt cttttccatc aattttgtga ccctggggtt ttatgccttc agccagatgt
     5401 actactttgg cggcggcatg atcaatgctc tcatcatcag cgccatcttc ctcgtatttg
     5461 agatcatcgt ctacgcttcg ttgatcgcgg tcatgccccg ttcaggcggt gactatgtct
     5521 ggatgacgcg tattttcggc ggcagcattg gcttcatcct tgccattacg ggttggtggt
     5581 tcaccctgtg gctgtggacc cctgtttatg gcgatatgtt ccgccaggtg gtattggtgc
     5641 ccatcatggg tgtgcttgga atgagagata ctgcggtgtg gttcgcgggt actggaacac
     5701 ccctctttgt cagttcactc attacccttg ccttcgtttc cctcgtcatc atccttggca
     5761 tgaaaaccta tgcacgcatt cagaagtttt ccttctatgc gggtgtgctt ggtttgttga
     5821 ttgtcattgt cctgcttttt acaggttccc ccgataaatt caaagctggc tttgagaaga
     5881 atgccgccgc cattttcggt tcccagcccg gtgtgtatga tgccaccgtg cagacgggca
     5941 ccgatgccgg cgcagccacc ccgcttacgg gcggctcttt cagcctcatc atggtgaccc
     6001 ttccgtggtt ggttttcttc aacttgtggc cgaattgggg cgcaaccttg tacggcgaag
     6061 ttcgcggcgc cacggattac aagcgtaact tctcaggcat ggcttgggcg cttggcacag
     6121 tgaccgtgct tggtattgtc ttcttcctcg gcgtggcgaa gaccatcggc tgggattttt
     6181 atatgcaagc caatggctcc tggtggaatt actattgggg ctacgttacc gatgtgcccc
     6241 cgttgcccgt atggccttac cctgccatgc tggccacatt cctcactgaa agccgcgcct
     6301 tgcaactcat cgtaatggtg ctgatgagct tctggtggtt cggttgggct ggtaccttgt
     6361 tcctttcctc tactcgcgtg gtgttcgctg cagcgtttga ccgcctgctc cccgaaaaag
     6421 tagctgaatt gaatgagcgc ggtacccccg tcaacgccat gttgttgatg gttatccctg
     6481 cagtgatcgt ttcctacatg tatgcctata acgttggcgg ttttatttcc gttacccttg
     6541 ccgctactca ggccattgcg atcatgtact ttggaacggc catcgcagct attgttctgc
     6601 cctataagaa gaaagatctc tttgaggctt cgccaatcgc tcagatgaag gttgctggta
     6661 ttccactcat caccattgca ggtgtgatct ttgccagttt ccttggcttc ctgctcgtgg
     6721 agtggttctt cgacccatgg ctcaacacgg gtggtgaagg cgcgggcctg tatggcatca
     6781 gcttcaagaa tacgaactcc gtcatcttcc tgctggttac ctacggcctg gctgccgcca
     6841 tttactacgg gttcaagtcc taccgtaaga agagcggcat tgatatggac aaggtccaga
     6901 ccgaaattcc tgtcgagtaa gtaatacaaa ctgggtgaca gccgccttgc atgggtggct
     6961 gtcacctttt tgatttccaa tgggagtgca gaatcatgcc agtgatagat gaagtggtct
     7021 ccaaaatgga agcatggaag gggagagatg tatccatcga acacctttca ggcggattaa
     7081 ccaacaccaa ttacaaggtc catgtagatg gccgtccgtt ttttatccgc gtgcctgggg
     7141 aaggcaccga attactggcc attgaccgca acaacgagta tcacaacagc agagccgcgg
     7201 ccaatgcggg tgtcgcaccg cagatcctgc atcacttccc ggaatacagt gtgatggtgt
     7261 tggaattcct aaatggtaag accatgtcga aggattccct aaatcagaag gggatgcctg
     7321 cacgcatggc ccagtccatc aagaaactcc atgcgggcga acgcttcttg accgacttca
     7381 acatgttccg ccttacggaa tattatctgc gcctgtgcaa ggaacgcgat atcaaaatcc
     7441 ctgatgggta tttggacagg ctggagaccg ttgccctcat tgaaaaagcg atgtcggtga
     7501 atcaacttgc gacggttccg tgtaacaatg acctgcttgc agaaaactat atagacgatg
     7561 gcaatcaact ttggctgatc gactacgaat atagcggcaa taacgacccg acctttgagc
     7621 tcggcaatac ctgtcaggag atgcaattca acgacgagca gatcagggag gtgtgtgcgg
     7681 catactttgg cgatgtctct cccgccatga tcgcacggat gaagctgaat atgatcatgt
     7741 ctgatgtggg ttggggattg tgggcagcca ttcaggagaa aatatccacc atcgagtttg
     7801 atttttgggg ttgggctttg gagcgttggg gcagggccca ggagaaaatg gactcgcctg
     7861 attttgatat ttggctcaag gatgtgcgac agtaacaaga taatcacacg ggcgcgcaag
     7921 cgcggaagca tgtatactta attaacctgt gttcgcgttt ccaattttca aaggatagaa
     7981 tcacttggat atcatacaag aaatgcaccg cctcattgat gcggccaatg gaaagaatat
     8041 tcaactgcgt gccatcggtg ggctggccgt gcagtctcac aacaagaaga atcacccctt
     8101 gttcctcagg gagtttgccg atcttgattt tgtggtcgcc aaaaaacagc gccgtgaatt
     8161 tgaggcattc atgcccgaag taggatacgc ccctcatcgg cagttcaatg tgttgaatgg
     8221 ggatcaacgg cagatctatt accacggtga gacggatatg aagatcgata tcttcgtggg
     8281 cgactttatg atgtgccata aaatcccgct tgaaaatcgc ttaaaagccg acccggtcac
     8341 cattccattg gcagagctgt tgctctcgaa agcccagatc gtccacctta accgcaaaga
     8401 cgctttggac attgcctccc ttctgctgaa tatcgaagtg ggtactggca acgataacat
     8461 catcaacttg gccgtcattg cacaattatg tggcagtgac tgggggttgt ataagactac
     8521 gtccataaac ctggagcggg tcaaggaagt ggtctctgcg gatgatgttg ccctgacctc
     8581 tgatgaaaaa tcattgatcg tttctcgtgt ggataaagtg cttgatgctt ttgccgccat
     8641 gccaaagact ctggcctggc agatgcgtga caaggtgggg acacgtgtac gctggtatga
     8701 ggaggttgag gaggtcgcac ggtgaataag cacctgcgct tgttctttgc cgtcctcatc
     8761 cttacactct ccatttcgtt gctggtttgg ggatacatgc cgaatccgca cgaaacacgg
     8821 attcaatgga tcgcgccgac agaaatgcag cttccatgaa agaggtactc aagccgctgt
     8881 atcggctggt cgtttttcct gtttttatgg ttgtattcat ttcccttgct tgtgttggca
     8941 atggaaacgc tccgtcctct gctagtgagc cgaccgcgat ctctgcactt gctacgcaaa
     9001 ctaccaacga agtttttcct gaacctgcac cagccatccc cgaaacccgc cgtcttacgc
     9061 ttgaattccc gccgaaaatg cgggcaggtg tggaaggtga tattgtccgc ttgactttag
     9121 aggtggatga cctgggaaac atcactccaa ccgctcaatt tgagggaaat gtagtgacgg
     9181 gtgaggtgat tgagattccc gatttatatg aaacgcataa tgtcaccgct gaagcccggc
     9241 ttgatatggc tgggatgcag attcagccct caggcgcgat ttacgagccg ctaaaacgcg
     9301 ggcaatctgc cacatttttt tggagtattc gcccgcagga cacaggggtt tatcgcggta
     9361 cggtctggct gcatttgaat tttacggaca tctcaacggg agaacaaagc cgaattgcag
     9421 tatcagcaca cacggtcgaa attgaagcgg tggatttttt cgggctgtct gtcaatgtgg
     9481 cgcgaacaac cggggtggtc ggttctgtgg tcggtggcat cctcggattc ccgtttttag
     9541 aggatattat caagttcctg ctgaagtttc ggagaaaaaa acagtctcca tcggcaaatt
     9601 aaatatatga cgaaaatcac ctaaaatatt gacatgcagt tatagggggt gatttgttct
     9661 atatggtaca attttcccat acaagatttt cgctatggag cacacaaatg aatgaatggc
     9721 tttacgttgg attgtttttt attgttggtt tgatcatccc cgtgggcgca attggtgttg
     9781 gctggctttt gagccccaag aagcccaatc ccatcaagca atcaacctat gaatgcggta
     9841 ttgaacctgt gggcgaaggt tgggtgcagt tcaaggctca gtattatatt tttgcactgg
     9901 tcttccttgt gttcgatgtg gaagccgtgt ttctttttcc ctgggctgtg aagttgaatc
     9961 agttggggtt gttcgcagtg gtcgaaggta ttattttcat tctcattctc gttgcaggcc
    10021 tggtttatac ctggcgcaag gggatgttgg agtgggctta gttttcaaca caataacgag
    10081 aaggctgaac ggatttttcc tttcagcctt ttctttgtgt aaagcaggta aattttcgta
    10141 gagcgaactg gcacttcgct ctacagattt acaggagtga cttatgaatt tttggaatga
    10201 ccctcttaaa atagccgcag attggctgac aggtatcttt gttgggtggg gcatgaatga
    10261 caccgccgcc catgtactgg ttgcctttct cggcgtgttg attctcatct ccattttgat
    10321 ggtggtggac attttccttg tgtggatcga acgcaaggtg gtggcgcgtt ttcaggatcg
    10381 cattggcccg aatcgccttg gaccttttgg cctgatccag ccattcgcag acatcatcaa
    10441 gctgatgatc aaggaagaca ccacgcccga tggcgcggac aaggttgtgt acaaccttgc
    10501 cccaatgctt tcgatgatgt cggtgttgat cctgtgggcg gtggttccgc ttgcgcctgt
    10561 gatgctcggc gtggacctga atgcgggtgc tttgttcatc attgccgcag gttccattgg
    10621 cacgctgtcc atcattatgg cggggtggtc gtcgaacaac aagttctcgg tcatcggcgc
    10681 attccgtcag gtggctgtga tggtttcgtt tgaaatcccg atgttggcga cattgctcat
    10741 cccgaccatc ttcgctggtt cgatggggat gaacgcgatc atccagggac aggatatctg
    10801 gtttgtgctg ctttctccgc tggcggcctt gatctttttg atcgcggcca ttgcagagtt
    10861 gggacgtgct cctttcgacc ttgctgaagg cgaatcggaa ctggtctctg gttacaacat
    10921 tgaatattct ggcatgaaat ttggcatgtt ctacgcaggc gagcttttgc atgcctttac
    10981 gtttggtggt ttctgggcaa ttttcttctt tggtggttat cgcttcttcg gtttggaaaa
    11041 ggtcagtcct ttccttgcga tcgcgatcat cctgttgaag gcgctcgttg gatactggat
    11101 catcatgtgg gtcaaataca cagtgatgcg tatccgtatt gaccagatgc tggctttcaa
    11161 ttggaaattc ctcacccctg ttgcgtttgt gctggtgatg gtaacagccc tgatgaatgc
    11221 gcttctgcgt gaagcttcaa gcgggcttta tatcacgggc atgatcctct cgaacatcat
    11281 tgtcggctgg gtctcgcttg agatcgtccg ttccatgagc aggaaagagc gcgagcgggt
    11341 ggaaggtgta aagcaggtgg tggaggctca ccactaaatc cttcgcatga gcgcggtttt
    11401 gatgatcggg tccaaaaacg ggacccttcg ctcagtgcga aacaggagat tttatgacag
    11461 cagaacaaat catttttctc attgttgcgg gcgtcaccct ggcttcggct ttgttggtgg
    11521 tgaccacacg caaccttgtc catgcggcgt tgtggctggt ggccacactt tttggtgtgg
    11581 cgatcattta cgccattctc aacgcaggct tcatcgcggt ggtgcaggtg gtagtgtata
    11641 tcggcgccat tgccattctt ttcatttttg cggtcatgtt gacccgcaga gagttgcgtg
    11701 acaatacccc gcagttgaac aggaattggt ggtcaggtgc gcttgtggcg gtgttgacct
    11761 ttgcaggcct cgcctcgctg ctttcgaggt ggagcggctt ctcgaagacc gcagcagccc
    11821 tcccgtcagg ttttgatgcg gtggccgagt tgggcaacca gcttgtttct cccgatgcat
    11881 ttgtcctgcc gttcgaagtc gcatccgttc ttcttctcgc ggcgatggtg ggcgcagttt
    11941 atgttgcgtt cacgcgcaaa taggaggctg aagagatgat cccactttcc tggtatctta
    12001 tttttgcggc agcgttgttt agtgttggtt tgtttggcgc attgtcgcgt aaaaatgccg
    12061 ttgcaatctt gctcggcatt gagttgatgt tgaacgcgat caacgtcaac cttgtggctt
    12121 tctggcgcta tggggatgtg gcggtgatgg ctgggcaggt tttcgccgtc atcgtgtttg
    12181 ctgtggccgc agcggaagtt tccgtcggtc tggctttggt catttctgtg tatcgccgcc
    12241 gcaatacggt gatcgcagat gaactcgata tgttgaagtg gtagggacgt attatgacac
    12301 atgaatttgt gacgaatttg gtttggctta ttcccttgcc acctgtgatt gcttttgcgt
    12361 tgatcgtgtt attcacgaac aagagcaagg ctttgagcca tacagtggcg atcagtgcgg
    12421 cgttcctctc ctggctgggt gccatgatcg tgttttggaa tgcggtaaag gttgagcatt
    12481 ttgcagaaga accttttgcg gcttccgtca actggctgcc gacaggtgaa acttggttga
    12541 aggtcggtgt gttgatcgac ccgctttctg cgaccatttt gttcttcgtg gcatggactg
    12601 ttttgatgat cttcctgtac agtgttggtt atcataactt cggccagcct gcaggcgacc
    12661 acgaccacaa gggcttgccc ccgcatggcg caactgtgac tgacgacctc ggccacaagc
    12721 acactgtgct ttcaattgag ccgatgtact cgcgcttctt tgccttcatc agcctgtttg
    12781 ccttcggtat gttgaccctc gtggtttccg ataacatcct gacccttttt gtgggttggg
    12841 agatcatggg cttgtgctca tatctgctca tcggcttctg gtacggaaaa gaatccgcgc
    12901 gcaatgcagc catcaaagcc ttcatgacca cccgtatcgg cgatgtcttc atgcttttgg
    12961 gtatttcatt cctgtacagc gtaacgggta cgttgtcctt ccgtgaaatc ttcaccgaag
    13021 aaattctgca cacccttgct tctgttccga gcggtgtgtt tggcctctct gcggcaggtt
    13081 tgatcggttt gctactcttc atcggtacgg taggcaaatc ggcgcagttc ccgctgcacg
    13141 tctggctgcc cgacgcgatg gaaggcccga ccccggtcag cgccatgatc catgcggcga
    13201 cgatggtttc ggcgggtgtg tatgcggtca ttcgcatgtt cccgatcatc agccttgatg
    13261 aaaagacgat ggcggttgtt gctttcatcg gtgcatttac cgcgctcttc gcggcgacca
    13321 ttgctgtggc acagaatgac atcaagcgcg ttctggctta ctcgaccatt tcacaactgg
    13381 gtttcatgat cgcagcactt ggcatccatg cctatgttgc ggctgtcttc catcttgtaa
    13441 cccatgcctt cttcaaggct ttgctcttcc ttggttctgg ctctgtcatt catggcatgg
    13501 aacatggcgt gatgcacaca ggcaatcaca aagttgaccc gcaggatatg ttcaacatgg
    13561 gcggcttgcg caagaaaatg cccatcacat tctggacctt cctcatcggt ggctttgccc
    13621 tttctggttt cccgatcgta accgcaggtt tctggtcgaa agatgaaatc cttgcggatg
    13681 ccttcggcca tggacacacc gttattttcg gcaccctcgc ctttgcagct ttcctgaccg
    13741 ccttctacac catgcgccag attacgctga ccttcctcgg ggaaccccgc acgaaggaag
    13801 ccgaacacgc ccatgagact ccctggacga tgacaactcc gcttgtagtg ctctcggtct
    13861 ttgctgtgac ctacggctgg gtcggcattc ccgagcattt cccactcctc ggcggactcg
    13921 tgccaaactg gttccatgaa tttgtcggct ctacgttaca tgaacaccct gaagccctgg
    13981 agttcagcat cgttcctttg ctcacctcgc tcggtgttgc tcttggcggc ttggggctgg
    14041 gctggctggt ttatcgcaac gtcaattctc ctgctgaaga caggctgcag attggtctgc
    14101 tcaagaacaa atactatttt gacgagattt acgatgttgt cttcgtcaag cctgcctact
    14161 ggcttgcaga gaacgttatc tacaaattta tggatcaagg tgtcattgac ggcattctcc
    14221 atttctttgg gccagccaca ggcggagttg gaagcacgat ccgcaattac tttgacattc
    14281 ccgttatcaa tcatctgatt ggtgatggtt ccgccgatgc gacctactgg atcggcaaga
    14341 acctccgtcc cattcaaaca gggcgtattc agcaatatct cattctctcc ctgatattgc
    14401 ttttcgtggt gggcggcctg gtctactaca ttctcacact ggcgtaggag catacgatgg
    14461 actttctcaa ttctcatctc cttagcctga tcttgttcct cccgaccgtt gcggcagtca
    14521 ttatgctctt cctgccgagt gaagaagcca aactccagcg ctggtttgca tttacggcca
    14581 gcctcattcc gtttggtctc accctctatt tgtggaacgc gtttgacccc aacagaacag
    14641 gctttcaatt tcaggaacaa tatacgtggt acgaagccat cggctccaac ttccaccttg
    14701 gcgtggacgg catatctctt tcgatggttt tgctcaccac cctccttaca ccattggcca
    14761 tcctcgcttc cttctcggtc agcgacaagg tcaaggcgta tttcatgctt ttcctcttcc
    14821 ttgagacagg tatgctcggc gtcttcatgg cgcttgacct gcttatcttc tttgtctttt
    14881 gggaaatcgg cctcgttccg atgtacttcc tcatcaatca atgggggagt gccaatcgga
    14941 attatgcctc gctcaaattc atgatctaca ccatggcggg gtcacttggc cttttgcttg
    15001 cagtgcagat gttgggcgtt ctcttcggga cctatgacct ggtcaccctt tacgaacgct
    15061 ggaattcgct ggatgctggt tctgtcttac tcggtatgcc agtgcatacc gtcaaaacca
    15121 tcgctttctg ggcgtttgtt gtcgctttcg ccatcaaagt ccctgtttgg cccttccata
    15181 cctggctgcc agatgcacat actgaagccc caacagcggg ctcaatgatt ttggcgggtg
    15241 tactgctcaa gctcggcgcg tatgggttta ttcggctggt actgcctttt tatccgcttg
    15301 aagcgaagat ctttgctggt gcgttggcct tccttgctgt agcggccatt gtctttggtg
    15361 cattttcatc ctatgcgcag acagacttca aacgattggt tgcgtattcc tcagtcaatc
    15421 acatgggatt cgtcatgctc ggcatcgctg cggcagggct ggcttctggc acgcaagatg
    15481 cacatatcgc catgagcggt gcggtgctgc agatgttcaa tcatggcctt tcggcggcag
    15541 gcatgttctt cctcgtcggt gttatttacg agcgcacaca tacccgcaac ctcaatgatt
    15601 tcggcgggct cttcccgctt gtgcctgtgt atggtggcat tttgatcttc accagcatgg
    15661 cctcccttgg cctgcccgga cttaatggct tcgtttctga gttcatggtg gttcgaggcg
    15721 cttaccccgt gttgaccaca tacacggcca tttccatgct tggcctgctt ttcacgggcg
    15781 cctacattct caagggtatc aagagtgttt tacacggccc cttgaacgaa cactgggcgc
    15841 atggtgaaca caaactgact gaaatcaaca cccgcgaaat cctggttatg gttccgttga
    15901 tggttctgat ccttgtcatt ggcgtctggc ccatgtggct tttggacgtg atcaacaaag
    15961 ccgtcacggc tttgttctaa gaggtgatgg atgacgaatt ttcccatcct aagtgttatt
    16021 accttccttc cgatccttgc ggctgccatt attttgatga tgcctgcaaa tcggaagaat
    16081 gaaatccgcg cagtggcgct tgccgctgcc acattagacc tgttgctctc tggctggctc
    16141 tatctgcaat acctgctggg agatatgaca ggctatcagt tcattgagca atataactgg
    16201 cttcctgcgc ttggtatcag cctgaaattt ggtgtggacg gtatgagcac cccgttggtg
    16261 ttgctcacgg gtattgtcat gttcacgggt gttctcattt cctggggtga cgaagacccg
    16321 catgtcaaag ctggtattca agatagaccg cgcgagttct tcgccttcct gttcctgctg
    16381 gcaggcggtg tgttcggcgt attcgtttcg ctcgacctgt tcatgttgtt cttcttctat
    16441 gaaattgccg tgttcccgat gtatttgctc attgccattt ggggctgggt gaagactcgc
    16501 gaatatgcag ccatgaaact gaccctgtac ctgttcatcg gttcagtggt cgcccttgtc
    16561 ggcgcactgg ccatgtattg gacccaatat cagaatacag gcgtactctc ttttgacatg
    16621 cttctgatgg aaaatgcgaa attttcgccg accttccagt atgtctggtt cctgcctgtt
    16681 ttcctcggtt ttgcggtttt gggtggtatc tggcctttcc ataactggtc accagatggt
    16741 cacgttgcag cgccgacggc ggtttccatg ttccatgcgg gtgtgttgat gaaactcggt
    16801 gcttttgccg ctcttcgcgt gggggtgatg cttctgcctg aaggtgcaaa agactgggcc
    16861 tggctcctga tgctttttgc aggcgttgcg gtggtttatg gcgcgttcat cgcctttgtc
    16921 cagaccgata tgaagtatat gatcggcttc tcgtctgttt cgcacatggg tttggtggtg
    16981 ttgggtgttt ccacactcaa tcatgacgga cttgtaggtg cgggtgtgca gatgttctca
    17041 cacggtgtga tgacggctct tttctttgcg gttacaggca tgatctatga ccgcgctcac
    17101 acccgtatga tccccgaact tggcggcatt gccaaagtga tgccatttgc cacggtcggc
    17161 tttatcatcg gcggcctggt ctcgatgggc atgcctggct tctcaggttt tgttgcggaa
    17221 tttcccatct tcatgggcgt gtggggacgc caatggctgg tggcagtgat cgccagcatt
    17281 tcaattgtca ttaccgcggc atacatcatg atcaacattc gcaaggtgtt tttcagcgag
    17341 atgcctgaaa acctccacgg tcacatcggt gatgtcacca tgctggataa aatcgccatt
    17401 acaacccttt gtctcttcat gatcgtcatc ggtatgttcc ctgctgtcat ggttcccatg
    17461 gtggaaaaag gcgttgagaa tgtgcttcgc ctgttgggag gtgcataatg tttacatcta
    17521 caactttcgc ttcgatcctc cctgaaacct tgatcctcat cctgggaatc atcctgttga
    17581 tcgttgagcc gttctggaag gctgagaacc gccgcaacgt tggctggctt accgctgttg
    17641 ggttgtttat tgccatgctg gtgagtttac ttttcggcca gcctggcgaa cccacctctg
    17701 ttcttggcgg catgatccgc tttgactggc ttggcttctt cttcaaaatg ttgttcatgt
    17761 tcgcgggcgc agctacagcc ctgcttctga tggatcatga aaaagcaggt ctgcgcggcg
    17821 aggcttacct catgctgctc gcttccatca ttggcatgaa cctgatggct gtttcttccg
    17881 atctggtgat gatgtacctc gccattgaaa cggcctccat tccgctctat attctttcag
    17941 gcttcctttt gggcgatgag cgttctacag aagcgggctt caaatacctg ctattcggca
    18001 cactggcctc cacggtcatg ctgtatggat ttagcctgat cttcgggttt tccggcacga
    18061 cgaacattta caaactctct gaaatgctcg ccgttggaga acttccgctc tttgttaact
    18121 ttggcctgat cggccttgtg gtggtgggtc ttggcttcaa gattgcggct gtaccccttc
    18181 acttctgggc tcccgatgtg tatcagggtg cgccgactcc tgtggcaggt tttctttcca
    18241 cggcttccaa ggctgcgggt tttgcagtga tcatacgcct gttctttgtg gcgtttcctg
    18301 aaattgccag ctattggacg gtcattttgg cggcccttgc cgcggtgacc atgacggctg
    18361 gtaacctgct ggctctctca caaacgaata tcaaacgcct gcttgcgtat tcttcagtcg
    18421 gtcatgcggg gtatgcgttg atcggggtgg tggccttcag ccagcttggc gctgcaagcg
    18481 tggttttcta ccttgcggct tatattgcga ccaatttgct ggcctttggt attgtcatgg
    18541 ctttcagccg tgtcgctggc ttggaagaca tcaccgatta cgctggtttg agccgccgca
    18601 atcccttgat gggtttgatg atgctggcgg ccttcctttc gctggcgggt atgcctccgt
    18661 tcggtggttt cgtggcgaag gtcttcgtct ttgccgcggg tgttcaggcc aattacacat
    18721 ggctggttgt ggtgggtatt ctcaactcga tcatcggtgt gtattactac ctgaacgtgt
    18781 tgaagtatgt gtatctctat cgcatgccga atgaagacga agaaaatcat cccattccgt
    18841 tgactcgtcc atacaccatt gcgctggtgg ttttggctct gggcgtgatc ttggtgggta
    18901 cggtctttgc tccgtggttt agctggtctg atgcggcggc tttgaatctg ttctaggaat
    18961 taggctttgg acgatgggca gtagactgcg gtttattgtc catcgttttg attaggtgta
    19021 gaaaaaaaca tagaaagcag agtcaggtca aaggtccaga cagatgcgct gtttgggcgg
    19081 ctggaaaggc aggttggcgg aagtccgtct gcagtgttga aaagggccgt tcggacggga
    19141 aatcccaatt tggatttctg tttgacagcc ctttgacctg tcagttataa tcgtgcgaca
    19201 tgacacaatc aaagcagacc aatcgtcaaa atatggtgta taccctgctc gtggttatgg
    19261 ttgggcaggt gggttgtttg accattgctg tgattttggc ctcggtattt ggcgggttgt
    19321 ggcttgataa tacttttgga acgaaaccgg tgtttacact ggtgttgttg tttgctggaa
    19381 tccctctctc ggtcttcctg atgattttcg cggcgcggaa aacgctggcg cggctgaagg
    19441 aaaaatacga aaccgaagag caaaaaacta ctgcttagga ggcgggcttg gaaacccaaa
    19501 aacgtacccc ctggcggcgc tggatcgtcc ttgtgctgat gatcgtgggc tttattctgg
    19561 gcggaatttt cgtccctgta cagcctgaga tcacggttgc cgccgaaaaa ttgatcgaag
    19621 aacccttgat cgagaacttt ctcggtcagc cgttctatct ggtgaacaca ctcccaaccc
    19681 tttttgttac tgtgttcatt atccttttgc cgcttgcctg gtctgctgga cgctctttga
    19741 agaagagtca ggagaccgac cttgtgccgc gtggtgtggg caacacaatg gaagccattt
    19801 tggaaatgtt gtacaacatg accgaaagct ccgctggcgt gaagtgggcc aagaccatct
    19861 tcccgtggtt tgctaccatt atgatctatg ttttggttgc gaacctgctc aaactgcttc
    19921 ctggctttga gtcgattggt gtgatccacc acgctcatgg cgaaggtcat ttaacgcaag
    19981 aattgggaaa cttcttaggc tttcgatggg aattgctttc ccctgataaa gttgaagaag
    20041 gtggctatat cctggctccg ttcttccgcg gtatttccgt ggatctgaac ttcaccctct
    20101 cgctggcgtt gatttccttc tttgccattc agatcatcgg cgtccgctca cagggcattg
    20161 gttatttcag caagttcttc aacacgaagc gcatgttcaa ggtgccgttc ttcggcgcga
    20221 tggatttcct cgtaggtttg ctggaaatca tctctgaact cgccaagatc ctctcgttta
    20281 cattccgtct tttcggtaac atgttcgcgg gtatcgtgtt ggtggcagtg gtggcttcat
    20341 tgcttggcaa gatcagcatt ctgcctgcaa tgatcatgat gtttgagttg ttcgtgggtc
    20401 tcattcaggc ctttgtgttc ggtatgttga ccatggtatt catggcaatg gcgacacagg
    20461 gtcatggcga tgaagaacac gctgaacact aagtcaaaaa attcaattca taaattatta
    20521 ggatacccct aaggaggctt gtaacatgga aggaaatatt ctcgaagcaa tgcgctttgt
    20581 tggcgcaggt ctggcgatga tcggcgctgc aggcgctggt gttggaatcg gtttgagcgt
    20641 gcagggagcc ctcacaggca tggcccgcaa ccccgatacc tatggtaact tgttgaccaa
    20701 catgattctc ggcgtggcgt tctccgaatc catcgctatt tattgtctgg tcatcgcgtt
    20761 cctcatgctc ttcaaagttg tgtaattcaa ggagcaacga aacatggaag ctcttggtat
    20821 taatctcgga ttgctcattg tccagttgat cgcgttcgca attgtgttcc tcacgctgaa
    20881 cgcctgggtg taccaaccca tgctggacat gatggaatcc cgcaaacaaa agatctcaca
    20941 gggcctcgaa gatgcccgtg ttgctgctga agcccgcgca aatgctgaaa aagaagccgc
    21001 caaagtcatc gctgaagctc aggctgaagc aagcaaggtg gttcgtgaag ctactgaacg
    21061 tgctgccgcc gctggcaagg atgtcaaggc tgctgctgaa gctgaagctg ccaaggcccg
    21121 cgaaaccgcg atggccgaag ccgaactcga acgcacccgc attcttggtg acctgcgcgg
    21181 acaggtttct gccctcgcca ttgccgctgc caataaactg gtcggtgaag ctttggatga
    21241 gaagaaacaa cacgccctca ttgatgaatt cttctctggc gtgaaggctg gcaaggtggt
    21301 ggttgtggat ggtgaattca agggcgatgc cgctgaagtc accagcgcac ttccgctcaa
    21361 caaccaggaa caggacgctg ttaagaagag ctttggcgcc aaggaagtat ccttcaaggt
    21421 caaccctgcc attctcggcg gtttggtcgt gaagatcggc gataaggttc ttgatggctc
    21481 cgtcgctggc aaactcgaag gtctgcgcca gactttgaag taagcctgta taaatgaaca
    21541 ccgaaagggt tttggtaaaa ttaccaaaac ccttttttgt ttaagcaatc ggagtgacat
    21601 gcttctaaaa aaactgttcg ttgattttcc actttccatg cctgaggtta tccccgatgt
    21661 ggaaatttca ggcattgcca ttgatagccg ggcggtaaaa cctggtcatc tttttgttgc
    21721 catgaagggt ggttttgccg atgggcatgc ctacattcag aaagccatcg aaaatggcgc
    21781 ttcggcagtt gtgggtgaga gggaattgga cgggctttct gtcccatatc ttcgactcga
    21841 aaacccgcgt caggccctga cctggctggc tgctgcgttt tacaattggc ctgcccgcaa
    21901 gctgacggtg atcggtgtga caggcacaga tggcaaaaca accacgagta atttgctcca
    21961 caagattttg ctcgcagcgg gaatcaaagc agggatgatc tccactgtta atgcagtgat
    22021 tggggatgaa gtgttggata ccgggtttca cgtcaccacg cccgatgcgc atgatgtgca
    22081 ggcttacctc gcccgtatgg tggaggctgg gttgacgcat gtggtgcttg aaaccacctc
    22141 tcatggctgg tcacagcatc gtgtggatgc ttgtgaattc gatatcggcg tagtcaccaa
    22201 tatcacgcac gaacacatgg atgaacacgg tagttatgaa aattaccgcg ctgccaaggc
    22261 gcgtttgttc tccagcctgg agtggactcg ttccaagcct gctggaaatc ctcgcctggc
    22321 ggtcatcaac cgcgatgatt ccaagtcatt tggatttttg gatgagttca tcaaagttaa
    22381 caaggtgaat tatgggttgg ggaaggatgc agatgtttgt gggatggata tcgagtacaa
    22441 ccccgctggc atccgttttg tggcaacctc caaagatttt cgtgtagccg catccagccg
    22501 tttggttggg gcgtacaatg tttcaaattg tttagccgct ttatcagccg ctgtgtatgg
    22561 attgggtatc aaacctgagg ttgcggcaca gggaatcgct gctttggagg gtatccccgg
    22621 gcgtatggag cgcattgatc tggggcagaa cttcactgcc attgtagatt ttgctcatac
    22681 ccccaatgca ctcaaagtga gcctggaggc aggcaggacg atgacacggg gaagggtcat
    22741 atctattttt ggttccgcag gcctgcggga taaagagaaa cgtcgcatga tggcagaaac
    22801 gtccgcagaa ttggcagacc tgaccatcct gacagctgaa gatccgcgga ccgagtcgct
    22861 ggatggcatt cttgaagaaa tggcggcagg cgctatagga aaaggcgggc gtgaagggga
    22921 gaccttttgg cgcgtacccg accgcggcga agccatcaaa tttgccttgc gcttggctcg
    22981 tgaaggggat attgtccttt cgtgcggcaa ggggcatgaa cagtccatgt gttttggcaa
    23041 aaccgaatac ttgtgggatg accgtaccgc tatgcgtgcg gcgcttgcgg attttttagg
    23101 tgtggatggt ccagcgatgc cctacctgcc ttcacgggat atgaatgagt cggattggtt
    23161 gaagggatga acgctgcggc tgtgagatta tgatccatac attgcatcgt gagcagatca
    23221 ttcccacttc tgtggaagtg ttgtgggatt atttttcggc ccctcagaat ttgaacgaca
    23281 tgacgccacc tgatatgaat tttcagatcg tgatgggtgg cgatgtgaaa atgtacgaag
    23341 ggcagatcat tgaatatcgc gtggagttca tccgcggaat ccgctcgttg tggctgactg
    23401 aaattgccca tgtccgcgag aaggaatatt ttgtcgatga gcagcgtgtt gggccttatc
    23461 ggttttggta tcacgaacac aagtttgagc ctgtggcggg cggcgtgaag atgaccgatc
    23521 atgtcactta tgctgcgccg ttcggatttt tgggtgattt ggtcaatgct gtttggattc
    23581 gtcgaagatt ggatgccatt ttcgattttc ggcatcgaag gatcatcgaa ttatttggga
    23641 gtgtgaaatg acaaaacctt gggcaactcc agtagtatta cagggaaaat atgtgcggct
    23701 ggagccgttg agcgaagcgc acgttgatgg cctggcagag atcggtgtgg gacaggattt
    23761 ttgggatttc atgttgtatg gccgcatgga aacgaaagaa gatatgcgga attgggtcaa
    23821 ggatattatg gggcgtggtg aaaaagggac cgatttgccg tttacggtca tccatttgga
    23881 ttcagggcgg gtggctggtg caacccgata tttgaatatc atgcctaatg accgtgggct
    23941 tgaggtgggc ggtacctggt atggacttga ttttcaacgt acgcctgtga ataccgagtg
    24001 taaatattta ttgttatccc atgcgtttga agtattgaaa tgcatccgcg tacagatcaa
    24061 aaccgattca cgaaatatcc gttcacagaa ggccattgaa aggattggtg cggtaaaaga
    24121 aggggtgctg cggaatcaca tgattctccc cagtggcata tatcgccatt cagtttttta
    24181 ttccatcatt gataccgaat gggaacaggt aaagaaaagg ctggaagcat tccttgaggg
    24241 gtgattttag cgattcgaag aaaaatcccc gtgtttattt cacggggatt tttgtattat
    24301 tgattaatgc cttttttgac ggttttcttt tgtgctatgc ggttacctgg ccgacattca
    24361 tgaacgggcc aagcagatac ctgaccacat cttccgctgt ataactggtt ttgccctctt
    24421 tgagaaactg cccgactgca acgaggtcat cgcctgaggt aatgatgggt tcttccacgt
    24481 aaccatcctt gcggacttgt ggaaagcctg cagtggctgt cagattgttg cacagacagg
    24541 agcggttgac cgtatcttca aggttgccgc cctttttgac aaaaacatcc acaggttcgg
    24601 aggcgcagcg atagcctaca gaaccatctt cgcgtttgta caagatgcga aggaaaccga
    24661 ggtcgcagat gcggttgcgg gcgctgtagg cctcagggtc agacagtgtg ccttcgagtt
    24721 gtgccacttt gaaagggaat cctgtaggag agaccagcgg actggtgaat acctgcgcct
    24781 cttcagcgcg gaccttttcg agcaaccgct gtttcaggtc ggggcgcatc cctgattctt
    24841 cacataggga aaaggcggtg ccaacctgca caccagccgc gcccgcttcc aaggcagact
    24901 gtacctgctt cggatttcca tagccgccag ccagccagaa aggcaggccg agcgccttca
    24961 ttttttccag atccacggcg tctttttcac cgtagatcgg ctcgcctttt tcattgtagt
    25021 tgaccgtgcc gcgaggcggg gcattgtgtc cgccagcggt ggggccttcg atcacgaaac
    25081 catccactga gccttcgctg cgtttgatga gggcctgtgc cagtaccacc gaggaaataa
    25141 tgggcaggaa cttgggccgc ttaagtttgc cgaccagctt ggcgatgcca gggaagatgg
    25201 cctcagggtc aaagtgaata cggtggtcat catccttgcc tgcgccgtga acatcaaggc
    25261 gatacgaaac aggctcatgg tttgaaagtt tgtcgaggat gccagcgatc tgggtgggaa
    25321 tccctgctcc catcagcacc acgtctacgc ctgcgagcat ggcgccgtag agcgaggcca
    25381 gggttggcat ctgcactttt tcgagcaggt taatgccaac caggccgttg tgaccttctt
    25441 tggcaaggaa aacttccacg taattggcga tcgccgtcac ctgatcgagg aatttgggcg
    25501 gtttgatggt gtaggccgca ggggttttgt atggggtttg cggtgatttt ccacctgggg
    25561 tgtaataccg atcaatgatc tgttgaacgg gctctggtaa agagaagttg gacagggcgc
    25621 ggcgtacatt tccgctgata tcgccatcca tgaggcggct ggctaaaact cggctgatgc
    25681 ccgtgccaga gaccaccccc aattgcccga ggtttgaaac agctttggca agcctccaat
    25741 cagaaatggc aacacccata ccgccttgga ttaattttgg tagattcatt cattgactcc
    25801 agtgattagt attaaatctg gtgcagggtt acatgttaat aacccctttg ggcggttatg
    25861 gttctgcctt tatgaaaaca ttctcagcct tcagcggcat cgtcgtaggg gtgttttgcg
    25921 agaatggcct cgaattcttt ttgttttcgc tcagcccagg caggcttgac catcattaaa
    25981 tattcaatat tcttggcgaa cagcaggcgg cggaaactct gtcctttgac cctgtgtttg
    26041 aattcatagg cacatgcctt gctccaggca atttcatcta caagccagta gagcaattcg
    26101 tttttgtcgc tggtttgctt ttcgctgagg atgagtccgc gctcggtggc gatgtaatga
    26161 taggagttgt ctctgatctc gaggtgcgaa gtcccatcat cgcctctgat ggtttggatt
    26221 ttgacatggg cataggcagg gtccatgcgg cggcaatact cgcggagctc ggcctggatt
    26281 ttttcgaagg atggctcgaa catggtcaac tctttcgggg tggagatgtt tcaggaagcc
    26341 gaatcaggtt tcggttgcgg gcgaagcgct gcctgggcga gggttctttt attaaggatg
    26401 agcagtcccg cagtgctggt catgccgatg agcagacctc cgatccagat ggcacgaggc
    26461 gagatgctgt cattgatgaa tccaccgata agcggcgaga gcgtgcgggc aaggccccaa
    26521 gccagccaat acaccgacat gtatcttccg cgcaggtcgg cgggggcggt gtcagccaca
    26581 tatttgcttg cagtggggac aagtgtaagt tcaccaaagg tgagcatcac catgcttaac
    26641 cagaatcccc aaaagcccga catgagcgcc acgcttccca cactgatggc gtagataaac
    26701 atgcctgccg cggccacggg cagggttttg tagtgcctcg tgattcgcgt gaccgcgtat
    26761 tgaatgaaaa cgcacatgac cgcattggtg gtggggatcc agccatacag ggcttcagat
    26821 aaactgaaat ttgtctttgc gtacacgggc atgaggatcc agaccatggt gggggcaatg
    26881 aggccgatgc cagtgaggac gacgaaggcc ataaaatcgc cgtctctaaa aacgcggacg
    26941 aatccttcca taacggaggg ttggtcggct aaagaatggc catttgttgc gccgacagtt
    27001 ttgtggagtg tttcatgtgc aaagagaaac atcagcaggc tatgggcgaa aaagcctaca
    27061 gcggcgccat aaaaagcgag atcgtatgag gaagctgcca gaaatccgcc gatggctggg
    27121 ccaagggcaa acgctgcatt gtgagcgatg cgattgatcg cgaatgcgtc ggtgcgttgt
    27181 tcggtaggga tgatatctgc catcatggca tcggcgccca cttgatagag cgggttgcat
    27241 aagccgatga agatcatcaa agcgatgaac tgtggatagg tttcagcctg cataagaaac
    27301 aggtaggcga tgccgtttac gaccaggctg aacaccatca ctgcttttcg gccgattttg
    27361 tctgtaagag tgccagccag gaaggaggcg agcaggcctg tccctgcgtt gatggaaatg
    27421 agggtggcta cagtgctgag gggcaggttg agttttccgc ctgcatagat aagcagaaag
    27481 ggccagatca tgcttgcgcc agccgtgctg atgacgatgc ctgccaccat caaccagaac
    27541 tggcgtggat attgattgaa gaggattttg agtttggaag ccatgggaag tcgagtctaa
    27601 catatcctca ctgtcgggtg aggaggtgga atggggcctg agatcaaagg tgaatggcgg
    27661 tgtttgttgg cgcgattttt ggatccaggt gtttttattt atttggagat cggtgtagca
    27721 aggtagtctt ccatctggcg ggtttcgtag acctcacgcc agactggttc aatggcttta
    27781 tggatggctt gataatttag actgtctttt cggatggcta ggccaaaatc gaaggcgcca
    27841 ggatagaact cgtactgctc ccaaaatgtt tgagaatcaa tgatctctgc ggtgatcttg
    27901 gtgtaatcga aatgaggaaa ccagatgagg tcaggtttgc ggcgcaggaa ttcgtctacg
    27961 gagaaaccgt tatgggcgaa gtaggaatcg tgcagtccaa taatgtcaac aatgtagagg
    28021 tgcggcgatt ccgcgcctgt aaatccatat tccgattggg caaaaatgat tccttcagga
    28081 agtgattttg agaatttggc taccgccaga accatgggcc atgttccgcg ttcaggaagt
    28141 tcttttgcgg cgggcgtggt gtattctgtg tgaggagagt acaactttgt ggcagcgatc
    28201 tgtgagtttt tgtatgcgtc actggtgatc gtttttagag tggagttgga aaagagtgcg
    28261 acaatcagaa ggagaagcag gactcgcatg agtcttttgc cgtgactttc tccttccgta
    28321 tctcctgaag aaagcgagcg gtcaagcatg aggatgctca gaacgatcag gtacgggagg
    28381 gcagggaaat aatatctggc ttcaaaaccc atgatctgaa caactgtgaa ataataggtg
    28441 aaggtcaggg caatggggag caaaaatacg gccagtaatt tcagagtctg ttttgtggat
    28501 gacaggacga tcaaaacaag aaaaggcagg gcaagggttc cgaaggcgaa tagatattcg
    28561 atcgggttcc aggtgtgcgt gccgatataa ccttcataaa agccgctgga cttggcataa
    28621 aacggcaggg gtagagggtc agagaagacg atgaacttca ccaacgtatc gagtgcaaga
    28681 agaacgccca gccaacctaa aaaatggaca agtcgttttc tcctgccttc aggagcaaga
    28741 aataggatcg ccaatactgg aaagaaaaat gtataaagca ggttgtcggg gcgtgccaga
    28801 aaggcaagat accctgcaat gaccgtcagg gttgaaaaaa gcctgtaccg attttcgttg
    28861 taaacccagc caagcgtgga gaagataagc agggcgttgc acaaaaaaga aagcgtggag
    28921 tccattccgc tcttcatgtg gaacaggtat acaggtgctg taaccagata gccaacaagc
    28981 agtacggtca accaaagaaa agagcttttc aggagttttg actcggcgta cctgctgact
    29041 gtataggcgg tcaacacaat ggcgggcagc ccaagaccag cggaaagggc aacaagcaat
    29101 ttgcctgatc ccaatccttg gaaaatgatt cgcgcaataa tgacgagacc cagatacagg
    29161 atacttgtgg ctccataggt ttgaacgcca tctgggttcc aggcaaagcc gtatccgtgg
    29221 atgaaattat ccgcgtaacg aatgaacatg tatgcatcat caaatgtggt tggccctgct
    29281 gttctgaaaa catcgatgta atatagcagc aaagcagcga ggatgattac cagagcggta
    29341 atacgcagtc tggggtttga aaagaaggag ggtttatcct tgaggtcgtt cacagcattg
    29401 actccaattg cttttacaga aatagcacat gtaaaattat agcactgtca taaaaaaagg
    29461 acgggtgaat gctttcaccc gtccttttga ttcctgagaa aatcttttat ctacgtccgc
    29521 cgcggtcccc gccacctcga cgatccccac cgcgatcgcc gccacggcct cccctgtctc
    29581 cgccgcgtcc gccacggtca ccaccgcgac ccccacctcc gccgccgcgc ccacccttgt
    29641 ctttttcacg ggcttcttcg gctgaccagc cttcgagtac agcctgacgc gaaaggcgga
    29701 tcttgccctg cgggtcgata tctgtgacca ttacggtcaa ttcgtcgccg agcacacaaa
    29761 catcctcgac tgtgttgacg cgttcactag ccagttgcga aatatgaacc agaccatcca
    29821 caccgggcag caggttgacg aacgcaccaa atgcctcaat gcgtacgacc ttgcctgtgt
    29881 aaatattgcc aacaattaca gactcgccaa gaccttccac gcgttcctgg gcgatctttg
    29941 cgcccacgct gtcggtcgaa gcaatgtaaa ctgtgccatc ttcctcgatg tcgatctttg
    30001 tgccagtctc ttcctgcagg gcgcggatat tcttgccgcc cgggccgatg agcgcaccga
    30061 tcttgtccac ggggattttc acagtgatga tgcgaggagc atgcggcttc aattctgccc
    30121 gtggggcatc cagcactgcc aacatctttt ccatgattga catgcgcgct gtgttggctt
    30181 gcgccaaagc ttccttcatc atttgcgcgc tcaagccgct gatcttgata tccatttgca
    30241 gggcggtgat gccctgtgca gtgcctgcca ctttgaagtc catgtcgccg aggtgatctt
    30301 cggttccctg aatatcagtc aatattttgt agcgtccagt ttcgtctgtg atgaggccca
    30361 tggctacacc ggaaacaggc gccttgatcg gcacacctgc atccatcaag gcaagggttg
    30421 agccgcagac cgaacccatc gaagtggagc cgttggaaga caaggcctca gacaccacac
    30481 ggatcgcata ggggaaggtc tcttcggatg ggatgactgg ctcaagtgcg cgttcagcca
    30541 acgctccgtg accaacctcg cgcctgctct ggccgcgaag gggtttcact tccccggtgg
    30601 agaatggcgg gaaggaatag tggtgcatgt atcgcttgct cttttcaggc gacaggttgt
    30661 cgagttcctg tgcttcaccc agggtgccaa gtgtggcaaa ggaaagtacc tgggtttcac
    30721 cacgggtaaa caggccggtg ccatgcgcac gcgggaagag acccacttca caccagatcg
    30781 ggcggatctc ggtgggggtg cggccatcag gccgcttgcc catgctcaaa attcgctcgc
    30841 ggacgatctt atgttcggct tcactgaacg ccgatttgac atctttttca gagggggcat
    30901 tctcatcgcc agccacgcag aattcggcaa tgatcgcggt ttgtaattca tccatgccgc
    30961 cgtagaactc agctttgctc aggggcttat cgagcaattc gttcatgcgg gcattgaccg
    31021 catctaagac cttcttttgc aggtcttcgt tggctgtgac gatcgtagcc tcacgcttgg
    31081 gtttaccgat ctcggcctgc atctgaagct ggaggtcaat gatcggctga atggatttgt
    31141 gtcccagttc cagcgcttca accatcacat cttcgggaat ttcgtttgca ccgcattcca
    31201 ccatgaggat ggcatctttg gtgcctgcaa ttctcagatc gaggtctgag gcgttcattt
    31261 catcgaaggt ggggttgagc acgaactcgc cgttgatgcg tcccacgcga acagcgccca
    31321 ccggtccgcc ccacggaatg tctgagatca taatggcggc ggaggcggca ttgatggcaa
    31381 gaatatccaa cgggttgaca ccgtcggatg agaaggtgta catgatgacc tgcacttcgt
    31441 tgcgcattcc atgggcaaag agcgggcgca gggggcggtc tgtgaggcgg ccagtgagga
    31501 tggcgtctgt tgcagcgcgg ccctcacgac ggaagaagga accggggatt tttccaccgg
    31561 catacatgcg ttcttcaaaa tccacagaaa gggggaagaa atcaatgccg tcacgtacac
    31621 cacccatggt ggctgcggca aaaacgatcg agtcttccac tccgaaagtg accgcaccac
    31681 ccgcctgagc ggccagtttg ccggtctcaa aggtgaaggt gcgcgtgcct acaacggctg
    31741 aatagcgttt tgattcgggt ttcattattt tattgtctcc tgttgcgcga gatacgatct
    31801 cgctgtacta attttccgcc aagggtcagg gactgaagag tgggaacaac ctcggttgtt
    31861 cgcgctattc aattcctgac ggcggtgcct atttacaata tagggacacg gcgtcgccgt
    31921 gtccctacgg gttaaaactt tttacttgcg gcggagctgt aagcggtcgg ttacactgag
    31981 gtagctctgg taactgcttg aacgcaagta cgtgagcaag cggcggcgtt gacctaccaa
    32041 cttcagcaaa ccgcggcggc aggattgatc ctgcttgttg cttcgcaagt gctcagtgag
    32101 ctgattgatg cgattagtca ggatggcgat ttgcacttca ggcgaaccgg tatccttgtc
    32161 gtggcgatga aaatcttcga tcgcctttgt cttgacttct tttgcaagcg gcatgacgta
    32221 tgcttccttt ctttttagat tacgcaggtc tccatgccca cagcttgaga ccatagggca
    32281 ggaccagtca cgaagcggga ttataccaga aaaccactcc cttggatgaa aaaaacgcct
    32341 cttttcgacc tgaaacctgc ttgcccttct ggaagcaggg tcagcgccga aggtgagtct
    32401 gttttacacc tcgatctgta ttgcactccc gctttcatag cggcgcagac ccatgcgaaa
    32461 ggtgaaaact gccaatgtaa ggaacccgat ggcccctaga agcagttctc cgagggtttg
    32521 ccaggtaaag gcacggataa actctgcagg gatggctccc atcagcgcag cagggataag
    32581 ggtaaaaagg ataagtttcg cgtagctatc gaaaagggtg atcgggtaga tgccaaaggt
    32641 tagcatggca ttgagtacga ggttactgaa attggtcatc atgccgaacc agaacgccag
    32701 actctgtgtc aggatgagaa atgccgcgaa aacagtagca gctaacagca tggcaagaac
    32761 gtagcggagc aatccatccc aggtgaaata tccagaaagt ccataactga cgaagccgta
    32821 cagaaaatct cccattccgc tggcgatggt tcggctggct atggtatgca acagaaccgg
    32881 gcggggcagg gagaggtagt aatcaaggcg acccttggcg ataatatcgc tgaggttgaa
    32941 ggcgtttccg aaaaaaagac tcacgaggcc aaaggcactg gccagcacac caaaggtgac
    33001 gtacatgtcg ttgacgcccc aaccgcgcac atctttgaaa cgatcgaaaa agatgatcca
    33061 gattgcgaag taaatgaaat tattgaccat catgccaatg gcttgcgtca ggaaggccgc
    33121 acggtattcc atggcagatt gcaggttggt tttccaaatg gcgaaaagga atttcagttc
    33181 tttcatatga cgatatcctg ctaacttgtt cgatgaggtc agttttttca gccgccattc
    33241 actgtcaatt gtgtcaaccc gcggcgatag acaatggtta gcgcaacgac caacgcaacg
    33301 atccatgtga cttgcagggt catgacgctg acaaaaagat cataggttgg ggtgacaaaa
    33361 agccgcgcag gcccataaat ggcgctggag aagggtagca attttgcgag gttttgcagc
    33421 cactgagggt aaaaatccag cggaatcaac atgccgccga aaatgaacgc cagcttttgg
    33481 tagatccacg taaatgcgga aacatcctcc acgacaaaag cggagagtcc gatcatggca
    33541 ttgatggcaa aatgcaaaac ccaggccccg agaatggcag gcagtgcgat cagaaatcct
    33601 tctgggtgat tgggtgtgcc caccagccac caaatggtca acccgccaaa tacggcattg
    33661 agcatggcac ggaagatggt ttcgcccatg gcagtgctga attggtagag caaaaaatcg
    33721 tatggcttgt tgagaatgta ggcaatggaa ccatctttga cgttttcaga catggtgcgg
    33781 gcaatgggtg gacgtcccag ttcgatggtc tctgccagca tgagatacca gagcgtgtcg
    33841 cgcagggtca atccattgat catgccatca acagccccag ccgcgctgaa ggtgaccttc
    33901 catagttgat agaagatcca cataaacggg atgatcatca aactacgccc aaccagctcg
    33961 ccaggatagg cgagcgagtt caaaagcgtg atctgaaaga tggaccagta tttggaaatg
    34021 gatttcatgg aaaagacagg aaatagtttg ttttgatcac agccgaagaa cactatggaa
    34081 aaaacactct tcgactgtgc ccaagaatgt ttctaaaatg gccgcatgct tgccgccgct
    34141 tcgagggcga gtgctgataa ctcctcctgg cggggcagtt ctttcatctg aaggatacgc
    34201 aggtgaatgg ctcgcaacaa gttacgggca ttgagataag ctcgggattg ttcctcatga
    34261 tcccatgcac ctttccagtc agttgtgagg ttttcgaacc aggccttgac ctgctcaagt
    34321 gatagttctt cgcgggcaag ggccgtgaca atggcacggg caaggcggtc atcttcgcca
    34381 tggatgtaaa tccaattttt ggcggcgcgc atcttttcag agacggcaga caggatgtgt
    34441 tccaattctg ttgcacccag gtgtgggctg cgggcaaata ccatcagcat atctgcggta
    34501 tgggctaatg catgtgccca acctttgacg ggaacgtatc cacgcgggtc acgttctgcg
    34561 gcgagatagg caaggacacg agtcagggtt tcttttactt catgcgactc cagatatggt
    34621 ttttggttgt cgcggtggat cacttctgcc agataaagca cggaaaatgc gcgcccaaat
    34681 acactgtcgt catcttgctg acccaggcca acttgcaggt tttcgagcag acgtttcagg
    34741 taaccgcgta attgctcggc ctggtagggc tcggtctcga tccagttggc aaagatttcg
    34801 tagccaatgc catcgcgtag atctgggtcg gtgctgggga tgtaggagaa taattcatcg
    34861 gtcaatgctg taaggtcgtg accctgaggg aaggcatatt tattttcctt gatcgtgtgc
    34921 cagaaatttt tgtccataat tgattgaaac gctaggcgtt ccctgcctcc tgataaattt
    34981 ttgaaatgac ctcttccagc ggcgggtcgg agatggtgat atccacgatg tcgcctgcgc
    35041 tggagaggtg agagatgacc gtatccacag gtgtttgggt tgtatcgaat ttcaacttga
    35101 caccataatg acccactttc aaggtttcca caccctgaat ggcaaaatct ttggagacct
    35161 ccaaggcata gcggacctcg accaattttg tggtgaggaa tttccgcttc aggtttgaga
    35221 ccttgtcttc gtacacgatc tgtccgtgat tgacgatgat gacgcgtttg cacagggctt
    35281 cgaggtcgcc tgcgtcgtgt gaggtgagca aaacgcccac gccttcctcc tgattcatgc
    35341 gtttgatggc atcgcggatg tgttgtttgg cgaccacatc aaggccgatg gacggctcat
    35401 cgagcaggag cagtttcggg cgatgtagaa gcgaggcagc cacttcgcag cgcatccgct
    35461 ggccgagtga gagtttccgc acggggacat ccagcaaatc cttgatctca aaggcttcgg
    35521 tgaggaagtc gatgcgtttt ttgatttcac gatcatccag ttcgtaaatt tttccgaata
    35581 gcatgaaggt gtcgatggcg ggcaggtgat accaaagctg gggcctttgc ccaaaaaccg
    35641 tgccaatgtg ataagccagt ttttgacgat cgacccaggg tgtgaaaccg agcacctttg
    35701 cctcaccgcc gctcgggtgc agaatccccg tcagcatttt gatggtggtg gatttgccag
    35761 cgccgttcgg accaatgaaa cccaacagtt cgcctgtgcc catttgaaaa gacaggttgt
    35821 gtaccgcttc aaccagactg tactctggct tgaaaagcga acgaatcgaa ccgctgagcc
    35881 ctgcggcctt gcgtttggtt tgaaaggttt tttgaaggtt gcttacatcg attgagagca
    35941 tgttattata attggcagta gaatcggatt gataattata gcgtgaaaga ggtattttaa
    36001 tgcgaaggct atcctgggtg attgtgttgg cgtttttgtt gcaggcatgc gcgggtttta
    36061 gtttaaaccc gcctacttcc acgccaacga ttacttcaac accaaccatt acgtatacac
    36121 caaccatcac tccgaccatt accattacac ccacccgcac agctttgcca accattgttc
    36181 gaatcccaac ctgggatccg aatcagccga cacccacttt tatggcgatt cccattttca
    36241 tcggcagtgt cactgcgacg ccgatcgttt cgccgacacc gacccgccca gggatcggat
    36301 ttgtttctgt ggacgtttct gagcagaaga tcttttgggg aagctgcaag cataacaagg
    36361 cgaagatcat tgcgaaggta caaaaccccg acgaagtttt cagcgtggtt atttttgtgc
    36421 gtgtaaaagc attgttgaag gaagattata cgccctggac cactggcgat gtcatgacca
    36481 aacatggtga tggaacattt tcctacaccc ttgtgggcag cgagatcgaa ggccataacc
    36541 attacaggga ttcgtgggtg tactttcaac tggttgcgac caatgaagaa ggtgaaattg
    36601 ttggccgaac gatgatttac accaattcac tttcgctctc gccctgcaaa taaacaaagg
    36661 atgcgatcat gaaaaagttt tatgcccttt ctctttgcac cattatgctg ttatcttgtt
    36721 cgttgaattt gttggcgccc agtataagtt ccacgcctgt gcctacgctg acaaacacac
    36781 ccaccgacct gccttccgct acgcccacag tgcccacacc tactttcacc cctacacctc
    36841 cattggttgg cttgaggacc catacactaa ccccaaccat cacggcaaca tccacgcagc
    36901 caactgaaac atccatcttt ttggccacac ttgatacgcc aacacccagc gtggaaatga
    36961 agggttttgt ttccgtcaat gtttcaacca aggagttttt caaagatgca gaatgtcagc
    37021 cgacttcagt gaagttcgtt gcccaggtgg ctgattcggc gggcaccgct tttgtggttc
    37081 tttttgtacg ttttaagagc aagcaaacag gaacgaccag cgaatggacc agcatcggca
    37141 tgtccacgat cggcgcgggc acatatacct acgaattgaa accagaagag atgaaaggtg
    37201 tggattcttt caagaacccg tgggtgcaat atcaatttgt ggcaaccaat gccgatgcga
    37261 acgaaatcgg acgaacggga attttcagcg aacgcttatc gctattggaa tgtgaaccga
    37321 cacccagccc aagcccatca cccgaaccga cggtgttgcg gccatgatgg aattccccga
    37381 tgacctgatc cgctttttga cgaaggccac ccgcgtctct gtcttaacgg gagctggtgc
    37441 ctcacaagaa agcggactcc gcacttttag agacgctcaa tctggacttt gggcacagta
    37501 caagcctcag gacctggcct cacctgaagc ctttgcccgc gaccccaaac tggtttggga
    37561 ttggtacgca tggcggcgtg aagccattaa aggcgtacgt ccaaatgcgg ggcattatgc
    37621 cttggtggat atggagaaaa aggttgaggt gtttacgctc atcacacaaa atgtggatgg
    37681 gctgcaccgc atggcgggca gttcgaatgt gctcgaactg catggaaata tcttaaaagt
    37741 acgctgttct gaatgcagaa catttacgga agtttggggt gacgattcgg aatctgtgcc
    37801 gctctgtgat aagtgcggcg gaatgctgcg ccccgatgtc gtttggtttg gagaggcgct
    37861 tccacgcgaa gaacttgagg ccgcggtcaa tgctgcgcgt tcgtgtcagg tcttcttttc
    37921 cattggaaca tcgggggtgg ttcagcctgc ggcagccttg gcccatgcgg cgcgcaataa
    37981 aggggccgtg gtggtggagg tgaacgcaga gccgactccg ctgaccccga aaaccgattt
    38041 tgcatttcac ggtaaatccg gggagattct gcctgagttg gttagggcag tttgggggta
    38101 gtttgagtcg ggctattcac ggccaggcga cttccaggta aatggtcacg ttgtcgtcca
    38161 cgcctatgtt ctccgatttt tgaacactca ttcgaatggg gacaaggtag cggccatctt
    38221 ttggaaatag ggaggttttc cattccgtgt ttccgatccg cacctgaact gggatgacgc
    38281 cccagccata tgtgacgctg gaggagatgg ctttcaaatc acggctgggt ttttctggga
    38341 cagccacaaa gagaaagggg gcagggccac gccagtgaaa aatttttcct ttgaatttga
    38401 tggtcataaa ttcagtatac caagcctttg aatgtactaa taaatatgtg atgttgatgt
    38461 tactttattg cctttcgggg gtattcatag aatttggctt tttcatcgtt gttgagttct
    38521 ttatagagaa gttcgagttc ttcaaagaca cgggaattgg gctttaccgc ttcatcatgt
    38581 gctgcgaacg ccagtttgat ctccagaagt gcatcttcgt agcgcccaag aaggtgcagt
    38641 gtttcccttg gctgtgtatt tatttatatt ttagtgcggt actgttctat tctcgcggca
    38701 atcctccaca gtaaccaaac aaggtaacta gcgtttaaaa tgatgaccgc tatataggtt
    38761 ggtctggttt catgaattac atcgtaaacc caaaggatat acaatccgca tgaaataaac
    38821 caaagaaaag ccagtgcgat catgccattc atcccctgtt tactaaggtt gttttttagt
    38881 tgaacaaagg aagatgcaat aatgaaagca atgcttacca cggcaacgaa tccgctaagc
    38941 ctgatcagta ccattgtcaa tgtgaagaag agttgaatgt aaaagaggaa aataacaagc
    39001 ccgacagata tgacaagcgc catcactgaa cttatcaacc agatatattc ctttctgtca
    39061 gtcctttttg catttactat tatatttgtt agcaagagga aaatggatac catactaata
    39121 gtaaagattt caggcccgac aatgccaatt gcatcaatta gattggcatc tttgtatttt
    39181 attggcccca atccgtatat ttctacactc tcccaaggac cgtcatccgt tttgaccagc
    39241 acgccttcgg atcccat
//
LOCUS       DAOQJC010000020        39152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_436_length_39152_cov_9.454997, whole genome shotgun sequence.
ACCESSION   DAOQJC010000020 DAOQJC010000000
VERSION     DAOQJC010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 39152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39152
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_436_length_39152_cov_9.454997"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1807
                     /locus_tag="PLE39_04625"
     CDS             152..1807
                     /locus_tag="PLE39_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67641.1"
                     /translation="MAAHIPSSMLLELGQAFSSMPELDPVLLSSLSQIQNIVASEGAA
                     LWLLNEAEVDIICTHATGADASKIMGSVMRARKFVAAYRTSTGRLTQTEDPLQSKWMD
                     SRAYRTYFNMSVRNMIGIPLVSRGKLLGEINIINKAGETAFTHNDYDFIHEVTQHIAL
                     AIQNTQLYERNNRTTGRQKLLNQISRHLHQTLDIDELIPRIFIEVNKAINAEAQSIWL
                     VDEEAGVIRCRFARGASAETLKGLSVPLNAPSIVGTSVSKQESIIIKDAQNDPRHARS
                     VDERTGFVTRSLMTVPLVLEDKSIGAIQAVNKRDGQLFTRDDLDLFRSIADSAALAVN
                     NAQLVADLQTSYDLTLDALSAALDLRDRETEGHSRRVVEYTTCLAQQIGLDKESIKNI
                     RRGALIHDIGKIGVPDAVLHKPGSLDETERNIINLHPLAGYNMLAGIPYLREEIQIVI
                     CHQEKWDGTGYPHGLRGEEIPIGARLFAIADTFDALTSDRPYRLGCSYETARGIIIEE
                     SGRQFDPRAVEAFLAIPEHEWMHIRAKVMEEIAHRRSLQKRVF"
     gene            1966..2370
                     /locus_tag="PLE39_04630"
     CDS             1966..2370
                     /locus_tag="PLE39_04630"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HNE67642.1"
                     /translation="MDVTLTTPALLFPTVSLLMLAYTNRFLTLASIIRNLHEKYKTDH
                     NENLLGQIANLRYRVYLIRNMQIVGVLSLLFCVVSMFALFAGWTLGGNWSFGIALILM
                     IISMLISLRELQVSVGALDLLLMDMEEHEKFG"
     gene            2417..3661
                     /locus_tag="PLE39_04635"
     CDS             2417..3661
                     /locus_tag="PLE39_04635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNE67643.1"
                     /translation="MEFNNSLSQLPNPSAKRIALRISPPAERALRQGHPWIFDQSITE
                     QSHQGAPGDLAVIFDDKRRFLAIGLYDPTSPIRVRILQSRDPATINTEWFQNKLKIAA
                     QTRTHLEKEKTTGYRLVHGENDGLPGLVIDRYADTLVLKLYTPAWIPHLKSFCDALAQ
                     VNTASHLILRLNRSLQKQTEFLHHLSDGMTITDQTSPSLILFQENGLTFESDPIHGQK
                     TGFFLDQRENRTRVEKFSKNKTVLNVFAYTGGFSVYAARGGAKEVTSVDISPPAIEAA
                     IRNMKHNQHFPTVASARHETFAEDAFEVLARMQSQKRLFDVVIIDPPMFAQSEKQTAG
                     ALSAYRKLTQLGLGVLRRGGMLVQASCSSRVDSDSFFESIHRSAQEAGRSLIEIERTG
                     HALDHPIRFIEGAYLKCLFATA"
     gene            3691..4815
                     /gene="tgt"
                     /locus_tag="PLE39_04640"
     CDS             3691..4815
                     /gene="tgt"
                     /locus_tag="PLE39_04640"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNE67644.1"
                     /translation="MFSFQITSKNNRARTGIFSTPHGDLVTPVFAPVGTQATVKTLTP
                     EHLKDIQASLVLSNTYHLYLRPGDELVRDMGGLHKFMQWPNPMLTDSGGFQVFSLSNT
                     RKIDDDGVTFKSHIDGSTHRFTPEKSIAIQENLGADIIMAFDECSDPNDLAYSKIAMD
                     RTHRWAERSLKAKTRPDQALFGIVQGGVNADLRAESAQFIASLGTPGIAIGGLSVGET
                     KQEMHDTLDVVMPLLPENKPRYLMGVGTPEDLINGVLRGVDVFDCVLPTRLARHHSAF
                     APEGRLNLMNATFARDERPIDETCDCYACKTFTRAYIRHLIVAKELLAGTLISIHNLR
                     ALIRLMEKIRLYIAEGTFESRAPELLDQWSNNAKRAGSGA"
     gene            4972..5709
                     /locus_tag="PLE39_04645"
     CDS             4972..5709
                     /locus_tag="PLE39_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67645.1"
                     /translation="MSKEKSFFRPIFYVVVVIYFILGFCTLALGEKWGDKLFLEDTYF
                     ENVGALSLGIASVISFYIFQQALKARTTANIHWFKLLVYLGLAMLYFFGAGEEISWGQ
                     RIFNIPEPENLPIENVQEELNVHNLAVFENSDILKADNIFTVFWVGFAAMLPALSLAS
                     KPFKAFAEKFTPIVHWDIGLLFIFNYLAAQVAKPIFRSFYSYQTISFVQAVQEVKESN
                     YEFLFIFLSIAVLWDLRQLIGKSGTSK"
     gene            5958..6791
                     /locus_tag="PLE39_04650"
     CDS             5958..6791
                     /locus_tag="PLE39_04650"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNE67646.1"
                     /translation="MSLFNVFILGIVQGLTEFIPVSSTAHLLVAQNLLKIPATDAAFS
                     FSVLVQMGTVVSLFFFFWKDLLSIALDTLKNLGNLKNFEQMPQNAKLGWYIVIATIPA
                     LIAGFFLRDSVQALFKTPLIEAAIRLFTASFLLAMAEFLDKKTRGLETMTWLDALIVG
                     LFQIIAVFPGASRSGTTIAGSMVRGFTREDSARFAFLMSIPVMLAAGSYEMLSVIERS
                     GTRAFLPYLLAGFLTSAAVGWLAIKWLMSYLHNNKHSLYVFSAYCGVAGIAVLFIQKL
                     P"
     gene            6791..7882
                     /locus_tag="PLE39_04655"
     CDS             6791..7882
                     /locus_tag="PLE39_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNE67647.1"
                     /translation="MKNLLKLLVETESPSHDKAAVDRVGKIVAEECRKLNAQVEILPN
                     EQTGDHVLARWGGTGKGGPLLLCHMDTVFPKGTTQTFTYKEVDGKIHGPGTLDMKAGI
                     VIALGGISALLSKGTAVPHPITALFTSDEEIGSFTSREYIEKLASEASIVFVMEGALM
                     DGSLKTWRKGVGEFRVKVKGRAAHAGGDHKEGRNAIEEMAHQVIAIQKLTDYEKQTTL
                     NVGIIHGGTVSNVVPEEANIVVDVRVMQPGEWERLEGEMKKLKPVLDGTSIEVSGELN
                     RPPLPFNEINQAAFKKAAQIAKETLGLELKAGGTGGASDGNFVAPLGIPVLDGMGAIG
                     EGYHSEREYIFADSLEERAKLLSVLIENW"
     gene            7913..8542
                     /locus_tag="PLE39_04660"
     CDS             7913..8542
                     /locus_tag="PLE39_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67648.1"
                     /translation="MKKFLILIFLLASCTPPAKTTTQPQIVTAYSTSAANPWLVELFS
                     CAESLNTAINISADSPDILLRVGEPEPLVSPAYQVGKDELLVIVHQDSPLQDLTLEEV
                     QSLFSAQENSTTQVWVYASDADMQVLFEQAVMEGRIVTSFAKVAVDSQRMLEAVESES
                     NSVGFILRGSVNSGQAYREVYSAGDVPVLALTQVEASGIIRDLISCISK"
     gene            8564..9907
                     /locus_tag="PLE39_04665"
     CDS             8564..9907
                     /locus_tag="PLE39_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67649.1"
                     /translation="MKPPLFTALLVIVFLVTACAQSPAPLATATIVPTPYPNVLFVDP
                     GISLGQISPLVYGTNYGPWIALSVEGLQPAYDSGVSIVRFPAGSWGDHNNVQTYQIDQ
                     LMSFIEKVGATAMINVRLLGGTPEQAAEMVRYVNKEQKYGVVYWGIGNEPSLYDGELK
                     NRGESYDIERFNQEWRAFAKAMKKVDSSIKLVGPETHQFSFDYNGASTNFSEANELWM
                     REFLKSNGDLIDIVSFHRYPFPRSFTSGPASIEELRVNAREWDKIIIHLRELIHTETG
                     RDIPIAVTEFNSDYSKSVGGESTPDSHYNAIWLADVLGQMIKNGVFMANHWMLTSKGG
                     NGGWGLIAQTDVNPSYFTYQMYKKFGDELVYSSSDIADLTVYASLRDDGALTVLVVNL
                     SLEEQIQSLRIAGQAAVKGEAWLFDPVHQAENIGTVDLSGNHSFPAQSITLFIIK"
     gene            9917..11134
                     /locus_tag="PLE39_04670"
     CDS             9917..11134
                     /locus_tag="PLE39_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67650.1"
                     /translation="MKRNLVILWAVWFTLLYGFQWLVTTRLQLKQPDEAVVWSANETL
                     PSSNDGKIYLLEPFMNRQVAWDSEYYVGIAVGGYDDPEAGYVRNPATGLPIAKNYSFF
                     PLYPYLMSAVAFPLRIFGLSAIGTATLAGSIITLLGTLAGLFAVWDLTQGLFDEDSAW
                     RAVFYMLVFPSAFFFAQIYTEGLFVGLAFSALMLTKRKQWVWASLLGFLAAWTRAHGA
                     AMALPLLLAWIINTDWKSNLRSQLTWKWFLQGLLAFLPLIGYFIWRTSPLGKGWEELQ
                     AFYFGRGLMTIESSLQSWKDAYFYARYVSQEGLIYFGIEVFTILLGLICSIWLIRRDP
                     IVALFSLAVVVLSVFSGSAQSMARYMLIAPSIFITLGYWGKNKVVDKAWTLISILLMG
                     MSVLLFSYDMWVG"
     gene            11337..12371
                     /locus_tag="PLE39_04675"
     CDS             11337..12371
                     /locus_tag="PLE39_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNE67651.1"
                     /translation="MTRKSDSVTIRQVAEKAGVSVATVSRYINHNAPISREVSDRLKK
                     VMAELRYVPHAAARHLASKKTRVIGLLLTNMHNDFFAPLLSGIESVVREHKYNLIVAT
                     NRADEETVVSPPVGSHNTDGLLVFADSLSDKVLIDLYNSEYPVVLIHRSSPPSVNIPS
                     VTVENKAATFHLMEHLIHEHKKRRILLMRGPIHQEDSYWRELGYKSALEANGIPFDDS
                     LVLQGDFERDVAYRSLNQFLGNGKRVAFDSVFAGDDDAAVGVIASLKEHGFRVPEDIS
                     VAGFDDVKISAYLTPPLTTVSAPTESVGRIAAEQLFSIFDKQLPEKVTLLPTELIIRH
                     SCGCSHEVNP"
     gene            12464..13855
                     /locus_tag="PLE39_04680"
     CDS             12464..13855
                     /locus_tag="PLE39_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNE67652.1"
                     /translation="MKKHLLALFSLMIVASMVLTACGGSAPAEKVKIRWFVGLGTGTD
                     PAQVTVQEEVVKDFNASQSDIELVLQIVPFDSARDTLSTQIASGEGPDIVGPVGWGGS
                     NAFYGQWLDIAPYIEETKFDTSVFDPALLKFYQTEEGQVGLPFAVFPGAMYYVPSMFD
                     EAGLAYPPQKYGEKYTLDGKEVDWNWETVTEVAKRLTIDVNGVNATEEGFDRTQIVQV
                     GYSAQWQHPNSVATFYSGAAKIYSGEKKGEYASALPDGWKEAWHWYYDGMYGDQPFIP
                     TGPLANSPEFGTGNVFNSGKAAMGLTQTWYTCCLGDFAKAGNEFQLGIQPMGSDGVVH
                     GRIDADTFRIWKGTKHPKEAFQVLAYLITTGGDKLLPAYGAMPAIASKTQAFFDKKAA
                     DYPFVTKESWDVFNQGLAYPDTPSAEQYQPNWKEAFDRQQVFMDLMNNTEGLDFDAEF
                     QKLVDDLNVIYNK"
     gene            13927..14952
                     /locus_tag="PLE39_04685"
     CDS             13927..14952
                     /locus_tag="PLE39_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNE67653.1"
                     /translation="MFEELANFRKYIPSIKSFRNASPLARRELKQGMAFLSPWLIGFF
                     AFTFLPIIASLIFSFLDLNITDGILNTPKFVGLANYIQLFNDRQAGVDPRTWITWLAG
                     GTPSSMGVTISFGLIALPVGILVPLGLAVLMNNPNLKGQMLFRSLFYMPFIVPFVASV
                     FLWGGMLNSETGWINRALIGLGVPKEVTPDWANSTTWVYPTYVIMGIWGVGNAMLIML
                     AGLQGVPTELYDAAKVDGANGWQTFWKVTFPMISPVIFYNLTLNIVGLFQYFLVPLVV
                     NQGTGRPGGATMFYSLYLFKNFFAFQDMSYGSTLAWFLFIVILLITFILFGTAKYWVY
                     YAGDSRS"
     gene            14965..16011
                     /locus_tag="PLE39_04690"
     CDS             14965..16011
                     /locus_tag="PLE39_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNE67654.1"
                     /translation="MHSKPLSPLQKRISIFTVTAFTFAILFIYLSPFAFMVLTSLKTQ
                     DQISIVGAPFYPAAPPKFEYNGDKLDVFVVPFPDGTTRELAMLKKGQKESTFIDPQNP
                     TGSEIVWQGSWRTLDRPWTFSPTWENYAEVWNNIDFPRLLWNTTFYAIMTEIGVLFSC
                     TLVAFGFSRFRFPGRDFLFVILIATIFLPAFVTVIPTYTFFAKIGWVGTWLPLIVPSF
                     FANAYDVFLLRQYFMTLPRELDEAAMIDGASPLRVLWSVIIPQSYPALMAVTVFHIVF
                     AWNDYFGPLIYLSTNRDNWPISVALSTFNGIYGQKPQLIQAGALMALILPLILFAVAQ
                     RFFVQGIVVTGVEK"
     gene            16156..17454
                     /locus_tag="PLE39_04695"
     CDS             16156..17454
                     /locus_tag="PLE39_04695"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HNE67655.1"
                     /translation="MLKVAVIGGGSTYTPELINGFLARLEKFPVNELWLMDIDKERLD
                     IVGGFAQRMVEAKGSPFKVVLTTDQRAAVKDADYVTTQLRVGHMEARKRDEYLGLRHG
                     LIGQETTGVGGMGKALRTIPVILKIAKDMQELSKPGAMLVNFTNPAGLVTQALSMYAK
                     DTPSVGVCNVPITAKMSILEGLEQATGRKIEPERAELKTLGLNHLSWHRGFTVDGEDV
                     WPQVIQGYIDELKKEEHPEWEPRTVEVLRMIPNYYLQYFYHTDKKLKAQEAWPPSRAE
                     EVIEVEKDLLRQYADPALNEPPADLMKRGGAYYSTVATQLLNAHYNDLGETHVVNIKN
                     NGAVKEWPAEWVLEMPSTVKKSGITPIAAEPLPTSCFGLISAVKAYELLTVEAAVHGN
                     RDAAYQALLVHPIGPKADKVQAVLDDMLETHREHLPQFFK"
     gene            17469..18434
                     /locus_tag="PLE39_04700"
     CDS             17469..18434
                     /locus_tag="PLE39_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNE67656.1"
                     /translation="MTYFLGIDIGATKTAALITNANGEPLGAGQGGPGNHETVGFDGM
                     EKAIREALNDALNTANIRMDAIAGAGFGIGGYDWPAEETAMRSAITNVGITAPFKMVN
                     DVTLGLLAGTSEGWGVAVVSGTGCNCRGWDRDHKREGRVSGYGTLMGEGAGASELIYR
                     TMQIIGYSWTKRLPPTLLSDAFIKHFNAKNLEDLIEGYTNGYYPIGADAAPLVFQTAE
                     SGDETAKKLVHWAGIELGELANAVIRQLEFESLEFEVALIGSMFKSGEALITPMRETV
                     HALAPGAKLIPAEVKPVIGAVLLGMEASGLKLTNSERTKIKAVLA"
     gene            18512..19372
                     /locus_tag="PLE39_04705"
     CDS             18512..19372
                     /locus_tag="PLE39_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HNE67657.1"
                     /translation="MTQKPSFRIVAYATDAITPSAIPYHQLTHINYSFLIPKADGSFV
                     NINNAWALEEIVAAAHENNVRVNIAVGGWGWDNEFEAMAAVPEARSAFVSNLAAFVEQ
                     YQLDGVDIDWEYPDQGQSAQNFLALMQELRAALPEKEISTAVISFGDDIGLGVPNEAF
                     ALVDFVNVMTYDGEIHGSLVEFKKGLEYWSARGVPKEKINIGVPFYSRPSEVTFAKLV
                     QFDPAAAQADAFEYNGSLENYNGIPTVQAKTELALQNAGGIMFWALDHDAQGQYSLVN
                     TIYQTIHTNP"
     gene            19402..20634
                     /locus_tag="PLE39_04710"
     CDS             19402..20634
                     /locus_tag="PLE39_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNE67658.1"
                     /translation="MSQPFTLGVNYWPRRKAMYWWSNFDAGEVREEFSLIKEIGLNVV
                     RLFLLWDDFQPDPASVAKDKIENLVKVADIAAEHGLGLDITFFTGHMSGPNWSPRWLL
                     GGDLPPKAHNWIRDIVSEGKKTDKGYRNMFHDEMALNATRLLLKTVVTTLKDHPAVWM
                     WNLGNEPDLFAWPNSSDEGAAWVKEMVGLIKSIDPNHPVTIGLHGDGLHRDNGLRIDK
                     VYAHTDVAVMHSYPMYTPWARQPLDPDFVPFTCALTAALAGKPVLMEEFGGCTALPGE
                     TTYTMKWTETNGREREQFMASEEDFAEFLRLTIPKLQHSGATGAMLWCYADYVPELWD
                     MPPCQNSQHERFFGLVRPDGSLKPHARVIQEFAKTNPQVLPIPDYAKLTVDADEFYKE
                     PLPHLVDLYQKYLTDLSK"
     gene            20663..21463
                     /locus_tag="PLE39_04715"
     CDS             20663..21463
                     /locus_tag="PLE39_04715"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HNE67659.1"
                     /translation="MKRLIINSDDYGRTPEVSRGIREAHLRGVVTSTTCMMNIPTTAA
                     DIQVALREAPLLGLGVHLVLTADRPIGPHESVRSITDEAGNFLNLSVLTQRIHSIEVS
                     EVKAEWRRQIEAFIQAAGKKPTHLDSHHHSSYFSAALFRAMLELAKEFDCAIRLPFME
                     GNDAIKEYAPALLAEFNPRRANTFYASFYDELATRAELQRIIASIQDGTCEIMVHPGY
                     ADEALLKASSYARQRETELEILTDPAIRQAIADRGIELIHFGSVEQDT"
     gene            21487..23958
                     /locus_tag="PLE39_04720"
     CDS             21487..23958
                     /locus_tag="PLE39_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNE67660.1"
                     /translation="MKFGYFDDKAREYVITQPDTPLPWINYLGSEAYFGLISNTAGGY
                     SFYKDARLRRLTRYRYNNSPLDMGGRYLYLRDNGTGKFWSPSWMPTRTKLDEYECRHG
                     QGYTIITSKFNGIKSSTRYFVPLGESLEIWQMTLTNESGSDSDLSLFSAIEFCLWDAN
                     DDATNFQRNYSIGQMEIVDEVIYHKTEYRERRDHFAYFACSEKLAGFDTIREDFLGHY
                     RSWNEPQVVESGRSSNTEAHGWQPIGSHQVKVNLKAGESKQIVFILGYVENPKDEKFD
                     PPQSQVINKKRAKIALEKYLKPENADQAFNTLREYWTGLLGKLQVNTPDEHTNRMVNI
                     WNAYQCMVTFNMSRSASYFESGIGRGMGFRDSNQDLLGFVHMIPERARERILDLSATQ
                     LKNGGAFHQYQPLTKRGNNDVGSGFNDDPLWLVLGVSAYIKESGDWSILDEMVQYENE
                     AGTETPLYEHLQRSIQYTLDRLGPNQLPLIGRADWNDCLNLNCFSDTPGQSFQTTTNK
                     EGKVAESVFIAGLFVLACKEMADLAGKMKEERGDSFVFHLSSDDYLKFATDMESTIWK
                     SGWDGDWFRRAYDDFGHVLGSKENSEGQIFIEPQGICIMAGLGLENGNAAKALDSVAQ
                     HLATPHGIVLQQPAFSQYYLHLGEISSYPPGYKENAGIFCHTNPWIMIAEALVGRGDA
                     AYDYYKRINPSAREEISEMHKCEPYVYAQMIAGKDAATHGEAKNSWLTGTAAWNYAAI
                     TQHILGIRPTLDGLEISPAIPASWDGFTATRQYRGVTYNITVENPRHVCKGVETVTVN
                     GRQVSGGLIRAENGETEVDVRVVMG"
     gene            23961..24824
                     /locus_tag="PLE39_04725"
     CDS             23961..24824
                     /locus_tag="PLE39_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67661.1"
                     /translation="MAESHLPIASIENDFLRLDFFTTTGPRIIGLYARGANGNLLAET
                     PDVHWPTPHGEFFLRGGHRLMTAPEDLFFMGPEDGLQMERSHNTVILKSPVDASSLER
                     EITIRLDENRAHLSHRITWHGKDEIRFAPWAITQMRMGGMAIVPQSNADGLQPNRNLV
                     LWPYTRIHDPRLELNDDLILLHGKVSAEACKVGTHTPLGWLACAMGDALFIKSFEVTA
                     GDFPDQGCNVEVYVKDTCIELETLGPLKTLKHGHSVTHSETWQVLTGSYPATLETART
                     ILRDHSNQDGA"
     gene            24828..26189
                     /locus_tag="PLE39_04730"
     CDS             24828..26189
                     /locus_tag="PLE39_04730"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HNE67662.1"
                     /translation="MANKITFPENFIWGAATASYQIEGAWNKHGKGESTWDRFTHTPG
                     KIRNNDTGDVANDHYRLWKKDIALMKKLGLKAYRFSVSWPRVLPTGRGKVNQKGLDFY
                     SKLVDGLLNAGITPFVTLEHWDLPQALEDKGGWTARATTEAFVEYADVMSRALGDRVH
                     QWITHNEPAVVAWLGYEMGVHAPGIKDLSASLRAAHHLLLSHGMSVPVIRNNSKDAEV
                     GITLNIGWKVPASNSKMDMDAARLDDGKWFRWFADPLYGRGYPSDMTKYFTQLGGAPN
                     GLDFVQSGDLETIAVPTDFIGLNYYGRNLHRVEDPNNGPQLFFPHPKTPEHWTEMDWE
                     NYPEGLTGILGRVYFDYTPLKIYITENGASYSTAPDENGNVPDVHRTNYLRTHFAAAH
                     RAMQAGVPLGGYFVWSLMDNFEWSFGYAQRFGIVWVNYETQERILKDSAKWYKTVIRK
                     NGF"
     gene            complement(26186..27382)
                     /locus_tag="PLE39_04735"
     CDS             complement(26186..27382)
                     /locus_tag="PLE39_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE67663.1"
                     /translation="MKPEQKSRFNDLFFARLYYFCFMGGWGFVLPFINLFYVSIGFNG
                     KQIGVISSVSAIVGMLVSPLWVSEVKKRPQARNILQAALILGGIGYYAIGLQSQFLWV
                     VVIIFLHALAASGIAPLSDSMAVTVSQEAGSGYGSVRVWGSLGWIFTVLSSGWLTARF
                     GFIAAFIGVALMWLTAASLVFLIQPRFFSAQPASAQVRPNLSAALQYIWHDKVLRGFA
                     LALIIFGIFNNGVVQFENVFLAELGATKQVISVAGIMSALVEIPFMLFSDRIVQRVGA
                     HRLLMVALTMTIFMRLSVLTFPAILTIMAVRFIGGVAFSFYTISFIKLVSSRTQPTET
                     GTVLAILSVTITGLVNIIASPISGAIFDVIGARWLYALSATGYVISIACLWWTRPEKQ
                     PAGSAG"
     gene            27453..28358
                     /locus_tag="PLE39_04740"
     CDS             27453..28358
                     /locus_tag="PLE39_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNE67664.1"
                     /translation="MSTGNTKRLRYDRRFGLLLIAPWVIGLLFFKLLPILASLGISFT
                     NFQLLTPEQTQFVGLENYARLFQDEAVGYVLFETIALGISTIPLQLAASIVLASMLAN
                     PRLKFGNTMRTLFFLPSIIPGVAIFFMWIGFADPTTGWLNKLILHPLGLTGFNNLYSD
                     GAVALLFSISSLWSVGPGMLIMLGALQGLPPEIQEAARVDGAGPLVRFFYITLPLISP
                     AIFFSIIINMIAVFGGVVLLDRGSVFSGSFSPYDGYVSYILFDKMDLGYAASLAWFFF
                     ILVMIVVVALFSSAHNWVYYPDREA"
     gene            28361..29437
                     /locus_tag="PLE39_04745"
     CDS             28361..29437
                     /locus_tag="PLE39_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNE67665.1"
                     /translation="MKTKSRHKWLEPHNYLSLQLWETLGSALFNLLIFFMLFVYLFPM
                     LFMVATSLMEGIQLRDRNAPAYPSQQVRFEYQGEEYLVYNVPINGGVRQLALVEPKQT
                     SSKFIDPQNPEDGLIPWKGSWRQLKGVYRFHVTFDNFKILFSSLRIPEMVKNTFIMTI
                     ISEIGVLLSSIVVAYGFSRFPLPGGNFLFYVLIATILIPEKVTLIPTYFFFARVLKWQ
                     GTWLPILIPFFFGNAVYIFLLRQNFKSIPKDLDEAAMLDGAGPLRILFQVILPQSWPT
                     VLTVSLLHFYYMWNETRQAALYLSTRRDLAPISFGVQSFQSLAPIQNQLQAGALILMI
                     VPVIVLIISQRYFMRDMVVTGMEK"
     gene            29442..30752
                     /locus_tag="PLE39_04750"
     CDS             29442..30752
                     /locus_tag="PLE39_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67666.1"
                     /translation="MFKNPNHRNIFGIWLGWALIMLAYHNLLPARLTVQPPDRALEWT
                     VTETMPGSQYGKIYLNEPFLNRHVSWDSEYYLAIAVGGYEDPSPTRIRSNTGETTESG
                     GFWPFVVPPQSAERPGISLSYAFFPFYPMMIRLFALPLSLLGMNAIATASLAGVIISM
                     LGTLAAMIALYEFARTELDESSGLRAAFYLLIFPSGFFLAEIYTEGLFVGLAFSSLLL
                     LRRGHRGWAALVAVLATFTRAVGVTLAIPLFISWIKDAEWLELDMEWKQIYYRGLPWR
                     ILVNGLIATAPIIAFFLWRISYYGLAFSLVEEEFFGRGLLSLGYTFIAWKDGFMEIFG
                     NNPNAAAYYFVEWLGIIIGFAACIAGFKRHPDLAIFGFLVVFLSFTSGPAQGMYRYVL
                     AAPPVFLFLSRLGKNPIFDRIWTIASMLLMSMMCIMYMFDMWAG"
     gene            complement(30827..31123)
                     /locus_tag="PLE39_04755"
     CDS             complement(30827..31123)
                     /locus_tag="PLE39_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67667.1"
                     /translation="MATGDFAFVTSLEPAQKDVFLNREKYDIVYASILEILRMHGPMT
                     FTQLGHRVEERLQDGFDGSVLWYFTIVKLDMEACGEIQRVVNSKPQLFSLNVNV"
     gene            complement(31140..31505)
                     /locus_tag="PLE39_04760"
     CDS             complement(31140..31505)
                     /locus_tag="PLE39_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE67668.1"
                     /translation="MSKRNIVHVEIPAANVEGAGKFYQELFGWKLQHMPEMNYTMWEA
                     GDGSGGGFPAVSAENPAGQVLVYIDSDDIEADLKKAEKLGGKVIFQKTEIPGMGWFAV
                     FHDPTGNAIGLYTSLNPAN"
     gene            complement(31628..31969)
                     /locus_tag="PLE39_04765"
     CDS             complement(31628..31969)
                     /locus_tag="PLE39_04765"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE67669.1"
                     /translation="MPERRTTPRKKFDYYLRVVDDDTQGILGHMVEVSPIGLQLETTA
                     PCPVEKDYYLRVELTPELADRPFIIFIARSKWCRIDEIEPNLYHVGFAMAEIMPDDRE
                     IFLRILARFGR"
     gene            complement(32150..33487)
                     /locus_tag="PLE39_04770"
     CDS             complement(32150..33487)
                     /locus_tag="PLE39_04770"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE67670.1"
                     /translation="MKFLDLLRLIFGNLGRRKARVALTAIGVVIGTAAVVILVSLAVG
                     LQKSATDQLYGIGDLTLIDVMPAYGVFYGGGYGAPMMGGGGGGGGGLDEPALITNSVL
                     EQFRAIPGVHTVIPRDYLNASTIISYRKLEAGMGFIGIDTDDLANIGLEASEGTTILS
                     RGSVVIGAMTPNNFYDPNLRPGQEPPLPPNLYNEQIQMVVMKWDQEGNVSQKIISLRV
                     AGVLTETGGEADYSIYLPLDLVRSLNEWANGVRINYNKTGYNQVIVKVDDPNQALDIA
                     DQITAMGYQAITPQAYLESINGYFLVLQIIFGGVGTIALLVAAIGIANTMAMSILERT
                     REIGLMKAVGATNRDVLSIFLGEAAGIGFIGGAGGVIIGWLAAQGINVLAIIYLAGQA
                     SEQGGPPPSVAVYTPVWLPVFALIFATIIGMLSGLYPALRAATMIPVQALKYE"
     gene            complement(33494..35209)
                     /locus_tag="PLE39_04775"
     CDS             complement(33494..35209)
                     /locus_tag="PLE39_04775"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNE67671.1"
                     /translation="MKFQSKYFVSILLAAAFLASWATPVYADPIISSIDPNLVFNDAP
                     STVTITGSGFESGAVVSLKNYGDLTTSFDGPNQLRAVVRPGLPAGSYTVIVTNPDATT
                     FEYPNGLTVADTSPLPTPTSAPFDRPQMVIGSYEVNVQRVRSGDDFKLNIDFGNAGTV
                     AAYNVQAVFTSADLIATKTGGVIFLGTIRAGDHNDATQPFIALDSIYGKSVVTTDVTL
                     TYYDSAGTEYTDKFTLSIPVGGSNVPYPTATPTGVNTAQLVITSYGSTVDPLQPGEQF
                     QLGLTVQNMGNTSAKNVTMIVGGGSSGDSSGTPQPGGVSGSGGEFTNFAPVNSSNIQS
                     LGDLPAGGQLQVFQSLIVNVSTNPGAYPMKISFSYINNKGEVVNDDQVITLLVYSLPN
                     VDVSFYRPPDPFFAGQPGALPIQIVSLGKRASVLGNMKIETTNGMVDPATTLVGSLDP
                     GGYFTFDTTLIPDVAGPMELTVTIEYTDDFNQPRTITRKLQVTVEEAFVDPALDPSLD
                     PSLGGGGGEFIPVEEETLLQKIWRGILGLLGLDSASPANNFPTEGPALEGEPLPMPAN
                     GGKGG"
     gene            complement(35230..35967)
                     /locus_tag="PLE39_04780"
     CDS             complement(35230..35967)
                     /locus_tag="PLE39_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE67672.1"
                     /translation="MTSPLIQIRDLKKHYQMGGTTVRALDGVSLDIEPHTFTVVMGPS
                     GSGKSSLLYLLGGLDRSTSGEITISGERLDQMDENTLALFRRRTMGFIFQSYNLIPSM
                     TALENVSFPMQFSGIPVSERNSRSRQLLEQVGLGERSHHRPTELSGGQQQRVAVARAL
                     VNDPSLVLADEPTGNLDTASGVTVMQLLSDLHRSGRTVLVVTHDPRMTRFATHKIFLL
                     DGHVVTEEEYQSASMDMMQTLSDSPSA"
     gene            complement(35982..36728)
                     /locus_tag="PLE39_04785"
     CDS             complement(35982..36728)
                     /locus_tag="PLE39_04785"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNE67673.1"
                     /translation="MTESNPPLPTRRFNFARILEVLLHPKRAFAEIAGETVSSWLTPM
                     LALSISSSLVVIVSGFLKARAAAMGEIQLPPDWQYWTPDMQNNFMQAQQATQGATFVY
                     IIPLLSALAGLWLGWLILSGLLHLGSTLMGGRGTMGSALNVVGWGGLPFLLRDVLRVV
                     FMLIVGHTIVSPGLSGFAGGAVFLSKMLSRVDIFVIWNAILLILGFGTADGLTRNKAI
                     LVVIVILVVILLALAGAGTLVSSFSSGAAF"
     gene            complement(36865..37275)
                     /locus_tag="PLE39_04790"
     CDS             complement(36865..37275)
                     /locus_tag="PLE39_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67674.1"
                     /translation="MKHLRLLFVSLVLVALLAGTVTTPSLAYPDSTTTIVKVPVKPNR
                     SLPNADTYDCTKATLYNRNGSYVATFTNPPASNGCTFIFNNVPVNQWYTVEFLMKYKL
                     GSPPYIVIRTSQSVYIAKPTLTTITTATLLYRPK"
     gene            complement(37356..38201)
                     /locus_tag="PLE39_04795"
     CDS             complement(37356..38201)
                     /locus_tag="PLE39_04795"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNE67675.1"
                     /translation="MTKTLLAVLAHPDDESFGLGGTLALYASRGYDVYYVCATRGEVG
                     TVDAEHLKGFKDTAEMRTDELMRAGKELGLKEIFFLGYRDSGMPGTEDNKHPDAQINA
                     PIDEVAGRVVKHIRTLKPEVVLTFDPIGGYKHPDHIHIHKATVLAFEKADDASFHPEA
                     GAAFKPKALYFQVFPKGLLKAAVRLMPLFGKDPTKFGRNGDINIKELAEVTFPIHVRL
                     NVASAAEAKQKASACHASQGGGQMRRGLMGFVTRMFGENEDYMQAYPPVAGNSKRKTD
                     LFEGI"
     gene            38455..>39152
                     /locus_tag="PLE39_04800"
     CDS             38455..>39152
                     /locus_tag="PLE39_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE67676.1"
                     /translation="MLSIHNLSKSYGIQPVLQNINFNISAGERIGLIGPNGSGKTTLM
                     RILAKLEQPDAGRGDVASTRPNLRIGYLAQGMDLDPRQTLHTALGLDPATQTDPAAEI
                     ESLALALSQNPNDSALQTQYDHALTKLSALNLQPETILAPLGLDHLPADTPIAHLSGG
                     QKTRLMLARVLLEEPQLLLLDEPTNHLDIEMLEWLEEWLNQFQGAALIVSHDRVFLDN
                     TVTSILELNPITHG"
BASE COUNT         9708 a        10771 c         9513 g         9160 t
ORIGIN      
        1 aactccgtga tctctgtggt ggaagagtcg ttagcccttc cttttagcca ctccccgaag
       61 aaaatacttc gcccgctatt tttttgattt aaatcgcagt atactgaaaa agcaccgatg
      121 tcgtcctatt tactggtttg gagaattttt tatggccgcg cacatcccat cctcgatgct
      181 ccttgaacta ggccaggcgt tctcatccat gcctgagctt gaccccgtat tactctccag
      241 tctttcacaa attcagaata ttgtcgccag tgagggggca gccctctggc tgctcaacga
      301 ggccgaagtg gatatcatct gcacccatgc caccggggcg gatgcctcca agatcatggg
      361 cagtgtgatg cgtgcccgaa aattcgttgc ggcgtatcgt acctccacgg gcaggctgac
      421 acaaacagag gacccgctcc aatccaagtg gatggattca agagcctatc gcacctactt
      481 caacatgtcc gtccgcaata tgatcgggat tccgctcgtc tcccgcggaa aactgcttgg
      541 cgagatcaat atcatcaaca aggctggcga gaccgccttc acccacaacg actacgactt
      601 catacatgag gtcactcaac acattgcgct cgccattcaa aacacacaac tttatgaacg
      661 aaacaaccgc accactgggc ggcagaaatt gctcaaccag atcagccgcc acctgcacca
      721 gaccctggat attgacgaac tcatcccccg catttttatc gaagtaaata aagccatcaa
      781 cgctgaagcc caatccatct ggctggtgga tgaggaagcg ggcgtcattc gatgccgctt
      841 tgccaggggc gccagcgccg aaacgctcaa aggattgtct gtgccgctga atgcccccag
      901 cattgtcggc accagcgtct ccaaacagga atccatcatc atcaaagatg cgcagaatga
      961 cccgcgccat gcccgttctg tggacgaacg aacgggcttt gtcacccgct cgttgatgac
     1021 cgtgccgctg gttctggaag ataaatccat tggcgcgatt caagccgtga acaaaagaga
     1081 tggtcagctt ttcacaagag acgaccttga cctgttccgc tccattgccg atagtgcggc
     1141 attggcagtc aacaacgccc aactcgtcgc agacctgcaa accagttacg acctcacgct
     1201 ggatgcgctt tctgccgcgt tggatctgcg cgaccgcgaa accgagggac attcacgccg
     1261 cgtggttgaa tacaccacct gccttgcaca acaaatcggt ctggataagg agagcatcaa
     1321 gaacatccgt cgcggggcac tcatccatga tatcggcaag atcggcgtcc ccgatgcggt
     1381 gttgcacaaa ccaggctccc tcgatgaaac ggagcgcaac atcatcaacc ttcatccgtt
     1441 ggcaggctac aacatgctgg cagggattcc ctacctgcgc gaggaaattc agatcgtgat
     1501 ctgccatcag gaaaaatggg acggcacggg atatccacac ggcctgcgcg gagaggagat
     1561 tcccatcggt gcgcggttat ttgccattgc agataccttc gacgcactca cctccgatcg
     1621 tccctaccga ctgggatgtt cctacgaaac tgcgcgaggg atcatcatcg aagaatcagg
     1681 caggcagttc gatccacgcg ccgtggaggc cttcctcgcc attcccgaac acgagtggat
     1741 gcatatccgc gcaaaggtga tggaagagat cgcccatagg cgcagcctgc aaaagcgcgt
     1801 tttttgaaaa cagggagtgt ctaaaagaat gggttttggt tgccacgaag tcacaaagac
     1861 tcgaaggcac aaaggattct tggtggtttt gtggcttggc gtctttgtgt ttaccccaaa
     1921 gacttagcca ctcccacgaa attctataga gatataattc ccagcatgga cgttaccctt
     1981 accacccctg ccctgctttt cccaaccgta tccttgttga tgctggccta caccaaccga
     2041 tttttaaccc tcgcaagcat catccgcaac ctgcacgaaa aatataaaac agaccacaat
     2101 gaaaacctgc tcggtcagat cgcgaacctg cgatatcgcg tttacctcat ccgcaatatg
     2161 cagatcgtcg gcgtactcag cctgctgttc tgcgtggtca gcatgttcgc cctgtttgca
     2221 ggctggaccc tcggcggtaa ttggagtttc ggcatcgctc taattttgat gatcatctcc
     2281 atgttgatct ccctgcgtga actgcaggtt tctgtcggcg ctctggatct gttattaatg
     2341 gatatggaag agcacgaaaa atttggatag cacattccat acagcagagg ggattgaact
     2401 gtataatgcc agcatcatgg aattcaacaa ttccctctcg caactcccca acccgtctgc
     2461 aaagcggatc gcgctgcgca tcagcccgcc cgcagaacgc gcgttgcggc aggggcatcc
     2521 gtggatattc gatcaatcga tcaccgagca aagtcaccag ggcgcacctg gcgacctcgc
     2581 tgtgatcttc gacgacaaac gccgctttct tgccatcggg ttatacgacc ccacctcgcc
     2641 gatccgcgtg cgcattttgc aatcgcgcga tcctgcaacc atcaacacag agtggtttca
     2701 aaacaaactc aaaatcgccg cccaaacccg tacccacttg gaaaaagaaa aaacaacagg
     2761 ctatcgactc gtccacggtg aaaacgacgg cctgcccggg ctggtgatcg atcgttacgc
     2821 cgatacgctc gtcctaaaac tttacacccc cgcatggatc ccgcacctca aaagtttttg
     2881 cgacgcattg gcacaagtca acacggccag tcatttgatt ctgcgcctca accgttctct
     2941 tcaaaagcag acggaatttc tacaccacct cagcgatggg atgacgatca cggatcagac
     3001 ttccccaagc ctgatcctgt ttcaagagaa tggcctcacg tttgaatctg accccattca
     3061 tgggcagaag acaggtttct tcctcgatca gcgcgaaaac cgtacacggg tggagaaatt
     3121 ttccaaaaat aaaactgtgc taaatgtatt cgcctacaca ggcgggtttt ccgtctatgc
     3181 tgcacgcggc ggtgccaaag aagtgaccag tgtggatatc agcccgcccg ccattgaagc
     3241 ggccatccgc aacatgaaac acaatcagca cttccccact gtagcctctg cccgacacga
     3301 aaccttcgcc gaagatgcct ttgaagtcct tgcacggatg caatcgcaaa agcgcctttt
     3361 cgatgtggtc atcattgatc cgcccatgtt tgcccaaagt gagaaacaaa ccgctggagc
     3421 attatcagcc tatcgcaaac tgacccagct tgggctgggc gttttgcggc ggggcggcat
     3481 gttggtgcag gcttcctgct ccagccgcgt agattctgat tcgtttttcg aatccattca
     3541 caggtccgca caggaagcgg ggcgcagctt aatcgaaatc gagcgcacgg ggcacgccct
     3601 cgaccacccg atccgcttta tagaaggcgc atacctcaag tgcctgttcg ccacggcata
     3661 gctgtcattt tcatcgctta taatcgctgc atgttctctt ttcaaatcac atcaaaaaac
     3721 aaccgcgccc gcaccggcat tttctccacc ccacacggag accttgttac acccgttttt
     3781 gcccctgttg gcacgcaggc tacggtcaag acgctcaccc ccgaacacct caaggacatt
     3841 caagcctcgc tggttttgag caatacttat catttgtatc tacgccccgg cgacgaactt
     3901 gtgcgcgata tgggcgggct gcataaattt atgcaatggc ccaaccccat gctcaccgac
     3961 tcgggcggct ttcaggtttt ctcactctcg aacacccgca aaatagacga tgacggtgta
     4021 accttcaaga gtcatattga tggttccaca catcgcttta cacccgaaaa atcgatcgcc
     4081 attcaggaaa acctcggcgc agatatcatc atggccttcg atgaatgctc cgaccccaat
     4141 gaccttgcct acagcaagat cgccatggac cgcacccatc gctgggcaga acgttccctc
     4201 aaggcaaaga cccgccccga tcaggctttg ttcggcatcg tgcaaggcgg cgtcaatgca
     4261 gacttgcggg ctgagtcagc gcaatttatc gcttctcttg gaacacccgg tatcgccatt
     4321 ggcggacttt cggtgggcga gaccaaacag gaaatgcacg acacgcttga cgtagtgatg
     4381 ccgctgctac ctgaaaacaa accgcgctac ctgatgggcg ttggcactcc cgaagacctt
     4441 atcaatggcg ttttacgcgg cgtggatgtt ttcgactgtg tgttacccac ccgcctggcg
     4501 cgtcatcatt cggcctttgc gcccgaaggg cggctcaacc tgatgaacgc aacctttgca
     4561 agagatgaac gccccattga tgaaacctgc gattgctatg cctgcaagac cttcactcgc
     4621 gcctatatcc gtcacctcat tgtggcaaag gaactgcttg ctggcacgtt gatctccatc
     4681 cataacctgc gcgccctgat ccgcttgatg gaaaagatca ggctttacat tgccgaaggc
     4741 acgttcgaat cacgtgcgcc tgaattgctc gatcaatggt cgaataatgc caaacgggca
     4801 gggagcggcg cgtaaaaaca caaggccttg aaatgcaaag gcaatcctat ttgggtcaac
     4861 tcaaggtatc atgacgtttg tataattaac gggtgccaag atgtcgatcc cagaataaga
     4921 catccaaccc tccaaacaaa gaaacaaatt ccagaaaaaa ggaatcagaa aatgagcaaa
     4981 gaaaaatcgt tcttccgtcc catcttttat gttgtggtcg ttatctattt cattttgggc
     5041 ttttgcactc tggccctcgg agagaaatgg ggtgacaagc tctttctcga agacacttac
     5101 tttgaaaatg tcggggcact atcgttgggc atcgcctcgg tcatatcatt ctatattttc
     5161 caacaggcat tgaaggcccg caccaccgcc aatattcact ggtttaagtt actggtctat
     5221 ctcggtctgg ccatgcttta cttctttggc gcaggcgaag agatcagctg gggacaaagg
     5281 atattcaaca tccctgaacc agaaaacctg cccatcgaaa atgtccagga ggaattaaac
     5341 gtccacaacc ttgcggtctt cgaaaacagc gacattctga aagccgataa catcttcacc
     5401 gttttctggg tcggcttcgc ggcgatgctc cctgctctga gcctggcctc caaaccattc
     5461 aaagctttcg ccgaaaaatt caccccgatc gtccattggg acattggctt gctgttcatt
     5521 tttaattacc tggccgctca ggttgcaaaa cccatcttcc gctctttcta ttcgtatcaa
     5581 accatttcct ttgttcaggc tgtgcaggaa gtaaaagaaa gcaattacga atttcttttc
     5641 atcttcctct ccatcgctgt attatgggat cttcgccaat taattggcaa gtcaggcaca
     5701 tcgaaataag agagtaaact ccagccagaa caaagctcag tcactcgtaa atgtaaacac
     5761 atgcaagtct gaaattttga cattttatgg gtaaagtcca ggcccaactt tgaatctgca
     5821 atatttatgc ctcgtccaca ttgcacaaac acagtgtggg ggaggcataa atacacaaat
     5881 ctttacccgc cagaatagac cgtaaattca tttgggccaa ccacttagcc caaatataaa
     5941 acaattaagg aaaaccaatg tctctcttca acgtgttcat ccttggaatc gtccagggat
     6001 taaccgaatt catcccagtc tcctccacag cccacctgct ggttgcacaa aacctgctca
     6061 aaatccctgc aacagatgca gcattttcct tcagcgttct tgtacagatg ggaaccgtgg
     6121 tctctctgtt ctttttcttc tggaaagacc tgttgtcgat cgcccttgat acgctcaaaa
     6181 atcttggcaa tctgaaaaac tttgaacaaa tgccgcaaaa tgcaaaactt ggctggtaca
     6241 ttgtcatcgc aaccattccc gcgctgatag cggggttttt cttgagggac tctgtacaag
     6301 ccttgttcaa aacgcctttg atcgaagccg ccatccgcct attcacagca tctttcctgc
     6361 tggcaatggc cgaattcctt gataaaaaaa cacgcggcct cgaaaccatg acctggctcg
     6421 acgccctcat tgttggtttg ttccagatca ttgctgtttt ccctggggcg tcccgttcgg
     6481 gcaccaccat tgctggcagc atggtccgcg gatttacccg cgaagattcc gcccgatttg
     6541 cattcctcat gtccatccca gtcatgcttg cggcaggctc ctatgaaatg ctcagcgtga
     6601 tcgaaagaag cggaacccgc gccttcctcc cctatttgct ggcaggcttc ctcacatccg
     6661 ccgccgtcgg ctggcttgcc atcaaatggc tgatgagtta tctccacaac aacaaacact
     6721 ccctctacgt cttctctgca tattgcgggg ttgcaggcat cgccgtccta tttattcaaa
     6781 aactcccatg atgaaaaacc tactcaaact actcgtcgaa accgaatcac cctctcacga
     6841 caaagccgca gtggatcgcg tcggaaagat cgtcgctgaa gaatgccgca aactcaacgc
     6901 gcaggttgaa atcctgccca atgaacaaac aggcgaccat gtcctcgcca gatggggcgg
     6961 taccggcaaa ggcggccctc tgctcctctg ccatatggat accgtctttc caaaaggcac
     7021 tacacaaacc ttcacctaca aagaggtgga tgggaagatt cacggccccg gtacattgga
     7081 tatgaaagca ggcattgtca tcgccctcgg cgggatctcc gcgcttcttt ccaaagggac
     7141 ggctgttcct catcccatca cagccctgtt cacctctgac gaagaaatcg gaagttttac
     7201 ttcgcgtgaa tacatagaaa agctggcgag tgaagcctcc atcgtcttcg taatggaagg
     7261 tgcgctgatg gatggttcgc tcaaaacatg gcgcaaaggg gtgggggaat tcagggtcaa
     7321 ggtcaaaggg cgtgccgctc atgcaggtgg agatcacaag gaaggccgca atgcaatcga
     7381 agaaatggca catcaggtca tcgccattca aaaattgact gattatgaaa aacaaaccac
     7441 cctcaacgtg ggcatcatcc acggcggcac ggtctcaaat gtggtgcccg aagaagccaa
     7501 cattgtagtg gatgtgcgcg taatgcagcc cggagaatgg gaaaggctcg aaggggaaat
     7561 gaaaaaacta aaacccgtac tcgatggaac ctccattgag gtatcgggag aattgaaccg
     7621 tccgccgctg ccgtttaacg aaatcaatca ggcggctttc aaaaaggcag cccagatcgc
     7681 aaaggaaacg ttggggctgg aattgaaagc gggcggcaca ggcggcgcct cagatggaaa
     7741 tttcgtcgcc ccattgggca tccccgtgtt ggatggaatg ggcgcgatcg gtgaaggtta
     7801 tcactccgaa cgcgaataca tttttgctga tagcctcgaa gaacgggcaa agttactgtc
     7861 cgtactgatc gaaaactggt gagttcggta tgggaagcag taaaatcaaa aaatgaaaaa
     7921 gttcctcata ttgatattcc tgctcgcatc ctgtacccca cctgcaaaga ccaccactca
     7981 accccagatc gttactgcct attccacatc tgcagccaat ccctggctgg tggagttgtt
     8041 ttcctgcgcc gagagcctga acacagccat taacatttca gcagattcac ctgatatcct
     8101 tctgcgcgtg ggcgaacctg agccccttgt atcaccagca tatcaggtcg gaaaggatga
     8161 actgcttgtg atcgttcatc aagacagccc attgcaagac ctcacccttg aagaggtgca
     8221 aagtttattt tccgcgcagg agaattccac gacgcaggtc tgggtctatg catcagatgc
     8281 ggatatgcag gttttgttcg agcaggccgt gatggaaggc agaatcgtta cgtcatttgc
     8341 aaaggtggca gtcgattctc aacggatgct tgaagcggtg gagtctgaat ccaatagtgt
     8401 gggattcatt ttgcgaggct ccgtgaattc ggggcaggca tatcgcgagg tgtattctgc
     8461 gggtgatgtt cctgtgctgg cactgaccca ggtggaggca tcgggtatca ttcgtgacct
     8521 gatctcttgc atatcaaaat aaaatctaca ttggagcatc gctatgaagc cccccctctt
     8581 taccgctttg ctggttattg tgtttcttgt tactgcctgc gcgcaaagcc cagccccgct
     8641 tgccacggca accattgtgc caactccata ccccaatgta ttgtttgttg atccaggcat
     8701 ctcccttggg caaatcagtc cactggtgta cggcaccaac tacggtccgt ggatcgcgct
     8761 ttcagtggaa gggttgcagc ccgcgtacga ttcgggggtc agcattgtgc gttttcctgc
     8821 tggttcatgg ggcgaccaca acaatgttca aacatatcag atcgatcaac tcatgagctt
     8881 tattgaaaaa gtgggggcta cagccatgat caatgtacgt ctgctgggtg gaacgcccga
     8941 gcaggctgca gaaatggtaa ggtatgtgaa caaggaacaa aagtatggag tggtgtattg
     9001 gggtattggc aacgagcctt ctttatatga tggcgagtta aagaacagag gggaaagtta
     9061 cgatattgag cgcttcaatc aagaatggcg ggcatttgcc aaggccatga aaaaagtaga
     9121 ctcaagtata aaattggttg gccctgaaac gcatcaattt agttttgact ataacggcgc
     9181 aagcaccaat tttagtgaag ccaatgaact ttggatgaga gagttcctga aaagcaatgg
     9241 cgaccttata gatatcgttt cctttcatcg ctatcccttt cctcgttcat tcacatcggg
     9301 acctgcgtcg atcgaggaat tgcgtgtcaa tgcgcgggag tgggacaaga tcatcattca
     9361 cctgcgggag ttgatccaca cggaaacagg gcgtgatatc ccgatcgcgg tgacagaatt
     9421 caattcagac tacagcaagt cagtaggcgg ggaatcgaca cccgattcac attacaatgc
     9481 catctggctc gctgatgtgc ttgggcaaat gataaagaat ggcgtgttca tggccaatca
     9541 ttggatgctc acgtccaagg gtgggaacgg cggctggggg ttgatcgccc agaccgatgt
     9601 gaatccaagt tatttcacgt atcagatgta taaaaaattc ggcgacgagc tggtctattc
     9661 atcgtctgat atcgctgacc ttacggtgta tgcctccctt cgtgatgatg gtgcattgac
     9721 cgttcttgtg gtcaacctgt cacttgaaga gcagattcaa tcccttcgaa ttgctggtca
     9781 ggcagccgtt aagggcgaag cgtggttgtt cgatcctgta catcaggcag agaacatcgg
     9841 cactgttgat ctatcaggga accattcttt ccctgcgcaa tccatcactc tattcatcat
     9901 caaataaagg aatactatga aaagaaactt ggttatttta tgggctgtgt ggtttactct
     9961 gttgtacgga tttcaatggc tggtgacaac ccgtctgcaa ttaaaacagc cagatgaggc
    10021 tgtggtttgg agtgccaatg aaaccctgcc aagcagcaat gatggcaaga tatatttact
    10081 ggagcctttc atgaatcggc aggtggcatg ggattctgag tattatgtgg gtattgcggt
    10141 cggcggatac gatgatcctg aagcggggta tgtacgaaac cctgccaccg ggcttccgat
    10201 tgcgaagaat tattcgttct ttccgttgta tccatatctt atgagtgcag tggcgtttcc
    10261 attgaggata tttggccttt ctgcaattgg cactgccact ttggcgggaa gcatcatcac
    10321 cttgcttgga acattggctg gattgttcgc cgtctgggat cttactcaag gcctcttcga
    10381 tgaagacagc gcctggcggg ctgtgttcta catgcttgta tttccttctg catttttctt
    10441 tgcacaaatt tatacagagg gattgttcgt tgggctggct ttttcggcgc tgatgctgac
    10501 caaaagaaag caatgggttt gggcatcttt attgggattt ctggctgcat ggacgcgcgc
    10561 acacggagcg gccatggctc tgcctttgtt gttggcatgg attatcaata cagattggaa
    10621 atcgaacctg agatcgcaat taacctggaa gtggtttctg caaggattgc tcgccttcct
    10681 gcctttgata ggctacttca tctggcgcac ttccccactg ggcaaaggct gggaagaatt
    10741 acaggcattt tattttggcc gcggtctgat gacgattgag agttcgcttc aaagttggaa
    10801 ggatgcttat ttctatgcaa ggtatgtgag tcaggagggg ttgatctatt ttggtattga
    10861 agtatttaca atcttgctgg ggttgatctg cagcatttgg ttaattcgcc gtgatcccat
    10921 tgtggcttta ttcagcctgg ctgtagtggt gctttctgtg tttagtggat cagcacaaag
    10981 catggcaaga tatatgttga tcgcaccttc cattttcatt actttggggt attggggtaa
    11041 aaacaaggtc gttgataaag catggacttt gataagcatc ttgttaatgg gtatgtccgt
    11101 gctgttattt agttacgaca tgtgggtggg gtaattatct gcacgctcaa gtaacaaaaa
    11161 agtggcaact ttggatacca aaaatctaaa ttgccacttt tttgtcttca taccattcta
    11221 tatttgagag ccaaaaacac ctcgattttt taagaatttg tgccttgacc tagctttgca
    11281 agtatgaaat aatgtaaccg gttactgtaa ctggttacat acggttatac gaagctatga
    11341 ctagaaaatc tgactccgtt actatcaggc aagtggctga aaaagcaggt gtttcggttg
    11401 caaccgttag ccgctatatc aaccacaatg cgcccatttc aagggaagtt tcagaccgcc
    11461 tgaaaaaggt catggctgag ctgcgatatg ttccccatgc ggcagcacgt catctcgcct
    11521 ctaaaaaaac acgtgtcata ggccttttgc tgaccaatat gcacaatgac ttttttgcac
    11581 ctctactgag cggcattgaa tctgttgtgc gggagcacaa atacaacttg atcgtagcca
    11641 ccaaccgagc agatgaagaa accgtggtct cccctccagt tggttcccat aataccgatg
    11701 gtttgctcgt gtttgcagac agcctttcag ataaagtatt gatcgacctg tacaatagtg
    11761 agtatccggt tgtgctgatt caccgctcct ctcccccatc tgtcaatatc ccatcggtga
    11821 ccgtggaaaa taaagccgcc acctttcacc tgatggaaca tcttattcac gaacacaaaa
    11881 aacgacgaat cctccttatg cgtggtccta ttcatcaaga agactcttat tggcgcgaac
    11941 tgggttacaa atccgctctg gaagcgaatg gaatcccctt tgatgatagc cttgtgcttc
    12001 aaggcgattt tgaacgcgac gttgcctaca gatccctaaa tcagtttttg ggaaatggaa
    12061 agcgcgtcgc ttttgattct gtattcgcag gcgatgatga tgccgccgtt ggggtgattg
    12121 cttcactcaa agaacatggt ttccgtgtcc ctgaagatat ttcagtggca ggtttcgatg
    12181 atgtaaagat ctccgcatac ctcacccccc cattgacgac tgtttcagcc cctacagaat
    12241 cagttggacg gatcgcagcg gaacagttat tttccatatt cgataaacaa ctccctgaaa
    12301 aagtcacgct tttacccacg gagttgatca ttcggcactc atgcggttgt tcgcatgaag
    12361 tgaatcctta aggaaggagg caggaacaac ctacacaaat agccaccaac cctatattcc
    12421 ataaatcgtc atttcaaatt ctactagagg agaaaatagc accatgaaaa agcatttact
    12481 tgcactattt agcctgatga tcgtcgcttc gatggttctc acggcttgcg gcggatctgc
    12541 tcctgctgaa aaagtaaaga ttcgttggtt tgttggtctt ggtactggta ccgaccccgc
    12601 ccaggtgacg gttcaagaag aagttgtaaa agactttaat gcatcacaaa gcgatatcga
    12661 actcgtactc cagatcgttc ctttcgacag cgctcgtgat accctctcca cccagatcgc
    12721 ttcaggcgaa ggccccgata tcgtcggccc agtcggctgg ggtggttcca atgctttcta
    12781 tggccagtgg ctggatattg ctccctacat cgaagaaacc aaattcgaca cctccgtctt
    12841 tgatcctgcg ctcttgaagt tctatcagac tgaagaaggt caggttggtt tgccgttcgc
    12901 cgtattccct ggtgcaatgt actatgtacc ctctatgttt gatgaagccg gattggctta
    12961 cccgccccag aagtatggtg aaaaatacac cctcgatgga aaagaagttg actggaactg
    13021 ggaaaccgtt accgaagttg ccaaacgcct caccattgac gtcaatggtg tgaatgcaac
    13081 tgaagaaggt tttgaccgca cccagatcgt tcaggttggt tatagcgctc agtggcagca
    13141 cccgaactcc gtcgccacct tctattccgg cgcagccaag atctattctg gcgaaaagaa
    13201 gggcgaatac gcttccgctc ttccggatgg ttggaaggaa gcctggcatt ggtactatga
    13261 tggcatgtat ggcgatcagc ccttcatccc cactggtcct ctggccaact cccccgaatt
    13321 cggcaccggc aacgtcttta acagtggcaa ggctgccatg ggtctgaccc agacctggta
    13381 cacctgctgt cttggtgatt tcgccaaagc cggcaatgaa ttccagcttg gtatccaacc
    13441 catgggttcc gatggtgtag tccatggccg tattgatgcc gacaccttcc gcatttggaa
    13501 gggcaccaaa cacccgaagg aagctttcca ggttttggct tacctgatca ccaccggtgg
    13561 tgacaaactc ctgcccgcct atggagccat gcccgctatt gcatccaaga cccaggcatt
    13621 cttcgacaag aaagctgctg actatccgtt cgtgaccaag gaaagttggg atgtcttcaa
    13681 tcagggtctc gcctatcctg acacccccag cgccgaacag taccagccga attggaagga
    13741 agcctttgat cgccagcaag tcttcatgga cttgatgaac aacaccgaag gcctcgattt
    13801 tgatgctgaa ttccagaagc tcgtagatga cctcaacgtt atctataaca aataatcctg
    13861 catgatatac ttttggcggg ccaacttctc tgttggcccg ccaacataat aaccccggga
    13921 gagactatgt ttgaagaact tgccaatttc agaaaataca ttccttccat aaagtcattt
    13981 cgaaatgcat ctccgttagc gcgacgggaa ttaaagcagg gaatggcttt tctttccccc
    14041 tggttaatcg gcttcttcgc cttcacattc ctcccgatta tcgcatcact gatcttcagt
    14101 tttctcgacc tcaacattac agacggcatc ttaaatacgc caaaatttgt ggggttggca
    14161 aactatattc agttgtttaa cgaccgtcag gcaggcgttg acccacggac ctggatcacc
    14221 tggcttgcag gtggaactcc cagttcaatg ggtgtcacca tcagtttcgg gttgatcgct
    14281 ttgcctgtag gaattctggt gcctttagga ctggctgttc ttatgaacaa tccaaacctg
    14341 aaaggccaaa tgttattccg cagccttttt tacatgcctt ttatcgttcc ctttgttgct
    14401 tcagttttcc tctggggcgg tatgctcaac tctgaaacag gctggatcaa tcgcgccctg
    14461 atcggtttgg gcgttcccaa ggaagtcacc cctgattggg ccaatagcac cacctgggta
    14521 tatcctacct atgtgatcat gggtatatgg ggtgtcggaa atgccatgtt gatcatgctg
    14581 gcgggtttgc agggcgtacc cactgaactt tatgatgccg ccaaagtgga tggcgcaaat
    14641 ggatggcaga ctttctggaa agtcaccttc ccaatgattt ccccggtcat cttttataac
    14701 ctcacattga atatcgttgg tcttttccaa tactttcttg tgccgctggt tgtcaatcaa
    14761 gggacaggca ggccaggcgg tgcaacgatg ttctacagtc tttatctatt caaaaacttt
    14821 ttcgccttcc aggatatgtc ctacgggtca acactggcct ggttcctctt tatcgtcatc
    14881 ctattgatca cttttattct gtttggcaca gcgaaatact gggtgtatta cgcaggcgac
    14941 agccgttctt aaggagagat acaaatgcac agcaaaccac tttcccccct tcagaaaaga
    15001 ataagcattt tcacagtaac ggctttcacc tttgccatcc tcttcattta cctctcccca
    15061 tttgccttca tggtgttgac ctcgctcaag acccaggacc agatctccat cgtcggtgcg
    15121 cctttctacc cggccgcccc gccaaagttc gaatataacg gcgacaaact ggatgttttt
    15181 gtggttccct ttcctgatgg gacgacccgc gaacttgcca tgctgaaaaa gggtcaaaag
    15241 gaaagtacct ttatcgaccc gcaaaaccca actggcagcg aaatcgtctg gcagggatca
    15301 tggcgcaccc tcgaccgccc gtggacattc tctcccacct gggaaaacta tgccgaagtt
    15361 tggaacaaca ttgacttccc gcgcctgctt tggaacacca ccttttatgc catcatgaca
    15421 gagatcgggg ttctgttctc ctgcacgctg gttgcattcg gtttttcacg tttccgcttc
    15481 cccggccgtg actttttatt cgtcatcctg atcgcaacca tcttcctgcc cgctttcgta
    15541 accgtcatcc ccacctacac gttctttgcc aagatcggtt gggttggcac atggttaccg
    15601 ctcattgttc catcgttttt cgcaaacgcc tacgacgtct tcctgctgcg ccaatatttc
    15661 atgaccctgc cgcgtgaatt ggacgaggcc gccatgatcg acggtgccag ccccctgcgt
    15721 gttctttggt cggtgatcat cccgcaatcc taccctgcat tgatggcagt gacagtgttc
    15781 cacatcgtct tcgcatggaa cgattatttc ggcccgctga tctatctttc caccaaccgc
    15841 gataactggc ccatctctgt tgcgctttcc accttcaacg gcatctatgg ccaaaagcct
    15901 caattgattc aggcgggtgc attgatggcg cttatcctgc cgctgattct gtttgcggtc
    15961 gcccagcgtt tcttcgtaca gggtattgtg gtgacaggcg tagagaaata attagcacac
    16021 caaacaaaag cgaaggacac aatgggcgct gaaaagggaa aagaggatga aaagctccct
    16081 ttcgtgtgcc agcagtgtcc ttcgcttttt aagtaatcgt cagttttcca tctccaacaa
    16141 accaaggata tatctatgtt aaaagtcgca gtgatcggcg gcggttccac gtacacgccc
    16201 gaactcatca atggattcct cgcccgcctc gaaaaattcc ccgtcaacga actctggctg
    16261 atggatatcg acaaggaacg cctcgatatc gtcggcggat ttgcccaacg catggtcgaa
    16321 gccaagggat cacccttcaa ggtcgtcctc accaccgacc agcgcgccgc agtgaaagat
    16381 gcggattacg tcaccaccca attgcgggtg ggccacatgg aagcacgcaa acgcgatgaa
    16441 taccttggcc tgcgccatgg attgattgga caggaaacca caggtgttgg cggtatgggc
    16501 aaggcgttgc gtaccattcc cgtcatcctc aaaattgcca aagacatgca ggaactttcc
    16561 aagccgggcg caatgctggt gaacttcacc aatcctgcag ggttggtcac ccaggcattg
    16621 agcatgtatg ccaaggatac gccctcggtt ggtgtatgca atgtccctat tacagcgaag
    16681 atgagcatcc tcgaagggct ggagcaggcc acgggtagaa agatcgagcc tgagcgtgcc
    16741 gaattgaaga cgctcggatt gaatcatctt tcatggcatc gtgggtttac agtcgatggg
    16801 gaagacgtgt ggccgcaggt gattcaggga tacattgacg aactcaaaaa ggaagaacat
    16861 cctgaatggg aaccgcgcac cgtggaagtg ctgcgcatga tccccaacta ttatttgcag
    16921 tatttctatc acaccgataa aaagctcaag gcacaggaag cctggccgcc ctcacgcgct
    16981 gaagaagtga tcgaagtgga gaaggatctg ttgcgccaat acgccgaccc agcactcaac
    17041 gaaccacctg ccgatctgat gaaacgcggc ggcgcctatt actcaaccgt agccacccag
    17101 ctgctcaacg cccattacaa cgaccttggt gaaacccacg tggtcaacat caagaacaat
    17161 ggcgcagtga aagaatggcc cgcagaatgg gtgctcgaaa tgccctccac tgtcaaaaag
    17221 agcggtatca ctcccattgc ggctgaacca cttcccacct cctgctttgg gttgatttca
    17281 gcggtcaaag cctacgaatt gctgaccgtg gaagccgccg ttcatggtaa ccgcgatgca
    17341 gcttaccagg ctttgcttgt tcatcccatc ggcccgaaag ccgacaaggt tcaggctgtg
    17401 ctcgacgaca tgctcgaaac ccaccgtgaa cacctgcctc aatttttcaa ataaccgaca
    17461 ctgacggaat gacttacttt cttggaattg acatcggcgc aaccaaaaca gccgcactca
    17521 tcaccaacgc aaatggagaa ccgcttggcg caggtcaggg cggaccaggc aaccatgaga
    17581 ccgtcggctt tgacggcatg gaaaaagcca ttcgcgaagc attgaacgat gcactcaaca
    17641 ctgccaacat ccgcatggat gcgattgccg gggcagggtt tggaatcggc ggctacgatt
    17701 ggcccgccga agaaaccgcc atgcgcagcg ccatcaccaa tgttggcatc acggccccct
    17761 tcaaaatggt caacgatgtc acacttgggc tgctggcagg cacaagcgaa ggctggggcg
    17821 tggccgtcgt ttctggcaca ggctgcaatt gccgcggctg ggaccgtgac cacaaacgtg
    17881 aaggccgtgt ttcaggctat ggaacactca tgggcgaagg cgcaggcgcc agtgaactca
    17941 tttaccgcac catgcagatc atcggctact cgtggacaaa acgcctcccc cccacactgc
    18001 tcagcgacgc cttcatcaaa cacttcaacg ccaaaaacct cgaagacctc attgaaggtt
    18061 acaccaacgg ctattacccc atcggcgccg atgccgcccc gctcgtcttt caaaccgccg
    18121 aaagcggaga tgaaaccgca aaaaagctgg tgcattgggc aggcattgaa ctcggcgagc
    18181 tcgccaacgc agtcatccgc cagcttgaat ttgagtccct cgaattcgaa gttgcgctca
    18241 tcggctccat gttcaaaagc ggcgaagccc tcattacccc catgcgtgaa actgtccacg
    18301 ctcttgcacc tggggcaaaa ttaattcctg cagaagtgaa acccgtcatc ggcgcggttt
    18361 tattgggaat ggaagccagc ggattgaaac tgaccaattc agagcggacc aaaataaagg
    18421 ccgtacttgc ctgatgaaat tccgcgcttc tttcctaacc ttgtgcctgc ttaccctttc
    18481 attgacattt tgcgtcccgc aaaattcggt catgacccag aaaccctcct tccgaatcgt
    18541 cgcctacgcc actgacgcca tcaccccctc tgcaattccc taccaccagt tgactcacat
    18601 caactactcg ttcctcatcc ccaaagcgga tgggagtttt gtgaacatca acaacgcctg
    18661 ggctcttgaa gagatcgtcg ctgccgcaca cgaaaataac gtccgcgtga atatcgccgt
    18721 aggcggctgg ggctgggaca atgaattcga ggccatggca gccgtccctg aggcaagaag
    18781 cgcattcgtc agcaatctcg ctgcgttcgt ggagcaatat caactcgatg gcgtggatat
    18841 tgactgggaa tatccagacc aggggcaatc ggcacaaaac ttcctcgcgc tgatgcagga
    18901 actgcgcgcc gccctacccg aaaaagagat cagcaccgcc gtcatttcat ttggtgatga
    18961 cattgggctg ggcgttccca acgaggcatt tgcacttgtc gatttcgtca atgtgatgac
    19021 ctacgacggc gaaattcacg gcagtctcgt tgaattcaaa aaaggattgg aatactggag
    19081 cgcgcgcggt gtgccaaagg aaaaaataaa tatcggcgta ccgttctact cgcggcctag
    19141 tgaggtcact ttcgcaaagc tggtgcagtt cgatcccgcc gccgcacagg ccgatgcgtt
    19201 cgaatacaac ggctctcttg aaaattacaa cggcattccc actgtgcaag ccaaaaccga
    19261 actcgccctg caaaatgcag gcggaatcat gttctgggct ttggatcatg atgcacaagg
    19321 ccaatactca ctcgtcaaca ccatttacca aaccatccac acgaacccct aatcacctct
    19381 cacccttctt cggagccaac catgtcacaa cccttcacac tcggtgtcaa ctactggccg
    19441 cgccgcaaag ccatgtactg gtggagtaat ttcgatgcag gcgaagtacg cgaagaattc
    19501 agcctcatta aagagatcgg attaaatgtc gtccgcctgt ttttgctgtg ggatgatttt
    19561 caacccgacc ccgccagtgt ggcaaaggac aagatcgaaa accttgtgaa ggtcgccgat
    19621 atcgccgccg aacatgggct tggactcgat atcaccttct tcacaggcca catgagcgga
    19681 cccaactgga gtccgcgctg gttactgggc ggcgacctgc cccccaaagc gcacaactgg
    19741 attcgcgata ttgtcagcga aggcaaaaag accgacaaag gctatcgcaa catgttccat
    19801 gatgaaatgg ccctaaacgc cacgcgcctg ttgttgaaaa ccgtcgtcac tacgctgaaa
    19861 gaccaccccg ccgtgtggat gtggaacctc ggcaacgaac ccgacctttt cgcctggccc
    19921 aactcatcag acgaaggcgc ggcctgggta aaggaaatgg tcggcctcat caaatccatt
    19981 gacccgaatc accccgtcac catcggcctg catggcgatg gcttgcaccg cgataacggc
    20041 ctgcgcattg acaaagtgta cgctcatacc gatgtggccg tgatgcattc ctaccccatg
    20101 tacacgccgt gggcacgcca accgctcgac cctgattttg tccctttcac ctgcgcactg
    20161 accgccgctc tggctggcaa acctgttttg atggaagaat tcggcggctg cacagccctg
    20221 cctggcgaaa ccacctacac catgaaatgg accgaaacca atggccgcga acgcgagcaa
    20281 ttcatggcta gcgaagaaga ttttgccgaa ttcctgcggc tcacaattcc caagctccaa
    20341 cacagcggcg ccacgggtgc aatgctctgg tgctacgccg attatgtccc tgagttgtgg
    20401 gatatgcccc cctgccagaa ttcgcaacac gaaagattct tcggcctcgt ccgccctgat
    20461 ggttcactca agcctcatgc cagggtcatt caggaatttg cgaaaacaaa tccgcaggta
    20521 ttgccgatcc ccgattacgc caaattgacc gtggatgcgg acgaatttta caaagaaccc
    20581 ctgccccatc tcgttgacct gtaccagaaa tatctgacag acctgagcaa ataaccatga
    20641 ccctgatttt cactcccgcc ttatgaaacg actcatcatc aactccgacg attatggacg
    20701 aacccccgaa gtctctcgag ggattcgcga agcgcatctg cgtggtgtgg tcacatccac
    20761 cacctgcatg atgaacatcc ccaccaccgc agcggatatt caagtggccc tgcgcgaagc
    20821 acccctgctt gggctgggcg tccacctcgt tctaacagca gacaggccca tcggtccgca
    20881 cgaatcggtt cgctccatca ccgatgaagc tgggaatttc ctcaacctct cggtgctgac
    20941 ccaacgcatc cacagcatcg aggtcagtga agtaaaagcc gagtggcgca gacagatcga
    21001 agccttcatt caagccgctg gcaaaaagcc gacacacttg gattcgcacc atcacagttc
    21061 ctatttcagt gcggcccttt tccgcgccat gctcgagctg gcaaaggaat tcgattgcgc
    21121 catccgttta ccgttcatgg agggaaacga cgccatcaag gaatatgcac ccgccctgtt
    21181 ggccgagttc aatccgcgca gggcaaatac cttctacgcc agtttctatg atgaactggc
    21241 cacccgcgcc gaattgcagc gcatcattgc ctccatacaa gatgggactt gcgagatcat
    21301 ggtccacccc gggtatgcag atgaagcgtt actcaaagca tcgtcctacg caaggcagcg
    21361 cgaaaccgag cttgaaattc tcaccgatcc agccattcgg caggccattg cagacagagg
    21421 cattgagttg atccactttg gcagtgtgga gcaggatacc tagaaagtga aaaggatttt
    21481 tgaattatga aattcggcta cttcgacgac aaggcacgtg aatacgttat cacccaaccc
    21541 gatactccct tgccgtggat caattacctt ggcagtgaag cctacttcgg tctgatctcc
    21601 aacacggctg gcggttattc cttttacaag gatgcgcgtc tgcgccgcct cacacggtac
    21661 cgctacaaca actccccgct cgatatgggc ggacgatatc tctacctgcg cgataacggc
    21721 acaggcaaat tctggtcgcc atcatggatg cccacccgca ccaagctgga tgaatacgaa
    21781 tgtcgacacg ggcagggcta caccatcatc acctccaagt tcaatgggat caaatcctcc
    21841 acccgttatt ttgtcccact tggagaatcg cttgagatct ggcagatgac cctgaccaac
    21901 gaaagcggat cagactccga cttgagcctg ttctctgcca ttgagttttg tctatgggat
    21961 gcaaacgacg acgccacgaa tttccaacgc aactactcca tcggtcaaat ggagatcgtg
    22021 gatgaggtca tctaccacaa gaccgaatac cgcgaacgcc gcgaccactt tgcctatttt
    22081 gcctgctccg aaaaactggc aggcttcgac accatccgcg aggattttct cgggcactac
    22141 cgcagctgga acgaacccca ggtcgtggaa tcaggccgat cgtccaatac cgaagcgcat
    22201 ggctggcagc ccatcggctc gcaccaggtc aaagtcaatt tgaaggcagg cgaatccaaa
    22261 cagattgttt tcatccttgg gtatgtggaa aacccgaagg acgaaaaatt cgacccgccg
    22321 caatcgcagg tcatcaacaa gaagcgcgca aagatcgcgc tcgaaaaata cctcaaaccc
    22381 gaaaacgccg atcaggcctt caataccctg cgcgaatatt ggacgggact gctcggcaag
    22441 ttacaggtca acacgcccga cgaacacacc aaccgcatgg tcaatatctg gaatgcctat
    22501 caatgcatgg tgaccttcaa catgagccgc tcggcttcct acttcgaaag cggaatcggg
    22561 cgcggcatgg gcttccgcga ttcaaatcag gacctgcttg gctttgtcca catgattccc
    22621 gaacgggcac gcgagcgcat ccttgatcta tcggctacgc aattgaaaaa tggaggtgcc
    22681 ttccatcaat accagccgct gaccaaacgc ggaaacaacg atgtcgggtc agggttcaac
    22741 gatgacccgc tttggctggt gctgggtgtt tcggcctaca tcaaagaaag cggcgactgg
    22801 tccattctcg atgaaatggt gcaatatgaa aatgaagcgg gcaccgaaac cccgctctac
    22861 gaacatttac aacgctccat tcaatacacc cttgaccgcc ttggccccaa tcaactcccc
    22921 ctcattggcc gcgccgattg gaacgactgc ctgaatttga attgcttctc cgacaccccg
    22981 gggcaatcct tccaaaccac caccaacaag gaaggcaagg tggcagaaag cgtgttcatc
    23041 gcggggttgt ttgtgctggc ttgtaaagaa atggcagacc tggcaggaaa gatgaaagaa
    23101 gaaagagggg attctttcgt ctttcatctt tcttcggacg actatctcaa atttgcaacc
    23161 gacatggagt cgaccatctg gaaatctggc tgggatggcg attggttccg ccgcgcgtac
    23221 gatgattttg ggcatgtgct cgggtcgaag gaaaacagcg aagggcaaat tttcatcgaa
    23281 ccacagggca tctgcatcat ggctgggctg ggacttgaaa acggcaacgc cgccaaagcc
    23341 ctggattctg tggcccagca tctcgccact ccgcacggca ttgtgctgca acagcctgcc
    23401 ttcagccaat attatttgca tctcggtgaa atctcgtcct acccgcctgg ctacaaggaa
    23461 aatgcgggca tcttctgcca caccaacccg tggatcatga tcgccgaagc attggtcggg
    23521 cgcggcgatg cggcctatga ttattacaaa cgcatcaatc catcggcgcg tgaagagatc
    23581 agcgaaatgc acaagtgcga accctacgtc tatgcccaga tgatcgcagg caaagatgcc
    23641 gccacacatg gtgaagccaa gaattcctgg ctcaccggca ctgccgcctg gaattatgcc
    23701 gccatcacac aacacatcct cggtattcgc cccacactcg acggtctgga aatttcaccc
    23761 gccatccccg ccagttggga tggattcacc gcaacccgcc aatatcgcgg cgtcacgtac
    23821 aacatcaccg tggaaaaccc caggcacgtc tgcaaagggg tggaaactgt cacagtcaac
    23881 ggcaggcagg tatcgggtgg tttgatccgc gcggaaaatg gtgagaccga ggttgatgtt
    23941 cgggtggtca tgggatagtc atggccgagt cgcatcttcc gatcgcatcc atcgaaaacg
    24001 attttctgcg tcttgatttt ttcacgacga cagggccgcg catcatcggt ttgtatgcgc
    24061 ggggagcgaa tggaaacctg ctggcagaaa cacccgatgt tcactggccc accccgcacg
    24121 gcgagttttt tctgcgcggt gggcatcgtt tgatgactgc gcccgaagac ctttttttca
    24181 tgggtcccga agacggcctg caaatggagc gcagccacaa cacggtaatt ttgaaaagcc
    24241 ctgtggatgc ttcgtccctt gaaagagaaa tcaccattcg gctggatgaa aaccgcgcgc
    24301 atctttcaca ccgcatcacc tggcacggca aagatgaaat tcgattcgcg ccgtgggcga
    24361 tcacacaaat gcgcatgggc ggcatggcga tcgtgccgca atccaatgcc gatgggttgc
    24421 agccaaatcg gaatctcgtg ctttggccct acacgcggat tcacgatccg cgcctcgaac
    24481 ttaacgacga cctgatcctg cttcatggaa aggtcagcgc cgaagcatgc aaggtcggca
    24541 cgcacacccc attgggatgg ctggcctgcg caatgggcga tgcgttgttc atcaaaagtt
    24601 ttgaggtgac cgcgggagat ttccccgatc agggctgtaa tgtggaagtt tacgtaaaag
    24661 acacatgcat cgaactggaa acgcttggcc cgctgaagac attgaaacat ggccactcag
    24721 tgactcactc agaaacatgg caggtactga caggcagtta cccagccacg ctcgaaaccg
    24781 cacgaaccat ccttcgggat cattcaaatc aagatggagc atagaaaatg gcaaacaaaa
    24841 tcaccttccc tgaaaatttc atctggggtg cggcaaccgc ctcataccag atcgaaggcg
    24901 catggaacaa acatggcaag ggtgaatcca cctgggatcg cttcacccac actccgggca
    24961 agatccgcaa caacgacaca ggcgatgtag ccaacgacca ttaccgtctg tggaaaaaag
    25021 atattgcgtt gatgaaaaag ctcgggctga aagcctaccg tttttcggtc tcatggccgc
    25081 gtgttctgcc aaccggacgc ggcaaggtca atcaaaaagg actcgatttt tacagcaagc
    25141 tggtggatgg cctgctcaat gcgggcatta cccctttcgt cactctcgaa cactgggatc
    25201 tgccgcaagc cttggaagac aaaggcggct ggacagcccg cgccaccacc gaagcctttg
    25261 tggaatatgc cgatgtcatg tcacgcgcac tcggtgaccg tgtccatcag tggatcaccc
    25321 acaacgaacc cgccgtggta gcctggctgg gttatgaaat gggggttcat gcgcctggta
    25381 tcaaagacct gtctgcttcg ctgcgtgctg cgcatcatct gctgctctcg catggtatgt
    25441 cggtgcctgt catccgcaac aacagcaagg acgcggaagt tggcatcaca ctcaacatcg
    25501 gctggaaggt gcccgcctcc aacagcaaga tggacatgga cgcggcccgc cttgatgacg
    25561 gcaaatggtt ccgctggttt gccgatccgc tctacgggcg cggttaccca tctgacatga
    25621 ccaaatattt cacccagttg ggcggcgccc ccaacggatt ggattttgtg cagtcaggcg
    25681 atctggaaac catcgcagtc ccaaccgatt tcatcggtct taattattac ggccgcaatt
    25741 tgcaccgcgt tgaagacccg aataatggtc cgcagctctt cttcccacac cccaaaaccc
    25801 ctgaacattg gacagagatg gattgggaga actaccctga aggtctcact ggcattttag
    25861 gacgtgtgta tttcgattac accccgctaa agatctatat cacagagaac ggcgccagct
    25921 attccacagc gcctgatgaa aatggcaatg tccctgacgt gcatcgcacc aactacctgc
    25981 gcacccattt tgccgccgcg catcgcgcca tgcaggcagg tgtaccgctg ggcggatatt
    26041 tcgtctggtc gttgatggat aactttgaat ggtcgtttgg gtatgcccaa cgctttggca
    26101 ttgtctgggt caattatgaa acacaggaac gcatcctcaa agacagcgcc aaatggtaca
    26161 aaacagtcat ccgcaagaac ggattttagc ctgccgaccc tgcgggctgt ttctctggcc
    26221 gagtccacca caggcaggcg atactgatca catagcctgt ggcagacaag gcatataacc
    26281 agcgggcgcc aatgacatca aagatcgcgc ccgagatcgg cgaagcgatg atattcacca
    26341 gccctgtaat ggtcacgctt agaatcgcca gcaccgtacc tgtctctgtg ggttgtgtgc
    26401 ggctgctgac gagtttgata aatgagatgg tgtaaaagct gaaagccacc ccgccaataa
    26461 aacgcaccgc catgatcgta agaatggcgg ggaaggtcag caccgaaagg cgcataaaaa
    26521 tcgtcatggt gagagcaacc atcagcagac ggtgcgcccc cactcgttga acgattcggt
    26581 cagagaacaa catgaacggg atctcaacca atgcgctcat gatgcccgcc acagaaataa
    26641 cctgtttggt ggcgccgagt tcggccagga aaacattttc gaactgcaca acaccgttgt
    26701 tgaaaatgcc gaagataatg agcgccagcg caaatccgcg caggactttg tcatgccaga
    26761 tatattgaag tgcagcagat aagtttggtc tgacctgtgc agatgcaggc tgtgcgctga
    26821 aaaagcgcgg ttgaattaaa aagaccaggc tggcggctgt cagccacatt aaggccactc
    26881 cgataaaagc ggcgataaag ccgaagcggg ctgtcagcca tcccgaagat aggaccgtga
    26941 agatccatcc cagcgatccc cacacgcgca cgcttccgta tccgctgccc gcctcctgtg
    27001 atacggtcac agccatcgag tcggaaagcg gcgcaatgcc cgatgccgca agcgcatgta
    27061 aaaatatgat gaccacgacc cacaggaact ggctctgcaa accaatggca tagtagccaa
    27121 taccgccaag gatcaaagcg gcctgcaaaa tattgcgagc ttgtgggcgc tttttgacct
    27181 cactcaccca cagcggtgaa accagcatac ccacaatggc gctgacagat gaaataaccc
    27241 cgatctgttt cccattgaag ccaatgctga cataaaagag attgatgaat gggagaacaa
    27301 atccccaacc tcccatgaaa caaaaataat acaaacgcgc aaagaagaga tcgttgaatc
    27361 ggcttttttg ttcaggcttc atagtttgat cgggcaggag tatatcactc aaagcccacg
    27421 ctaaatttat cggaagatct gcaaaggaaa ccatgtccac tggaaataca aaacgcttga
    27481 gatatgacag acggtttggc ctgctattga tcgctccctg ggtcatcggt ctgctatttt
    27541 tcaaattact cccaattctg gcttcgctgg gaatttcatt caccaatttc caactgctta
    27601 cccccgagca aacacagttc gtcgggctcg aaaattacgc ccgcctgttt caggatgaag
    27661 ccgttgggta tgtattgttt gaaaccattg cattgggcat ttcaaccatc cctctgcaat
    27721 tggcggcatc catcgtactg gcatccatgc ttgccaaccc gcgtttgaaa ttcggcaaca
    27781 ccatgcggac tcttttcttc ctgccgagca tcatccccgg tgtagccatt ttcttcatgt
    27841 ggattggctt cgccgatcca acgacgggat ggttgaacaa gctcattctg catccgcttg
    27901 ggctgacagg attcaacaac ctgtactccg atggcgccgt ggcactgctc ttcagcatta
    27961 gctcactctg gtcggtgggg ccaggcatgc tcatcatgct tggtgccctg caagggcttc
    28021 cgcctgaaat tcaagaagcc gcacgggtgg atggcgcggg tccgctggtg cgttttttct
    28081 acatcaccct gccgctcatt tcacctgcca ttttcttttc gatcatcatc aacatgatcg
    28141 cagtatttgg cggtgtggtt ctgcttgatc gcggcagcgt attcagcggc agtttttcac
    28201 cttacgatgg gtatgtcagt tacatcctct tcgacaaaat ggatctgggc tatgccgcca
    28261 gtctggcgtg gttctttttc attcttgtca tgatcgtggt cgtggcgctg ttttcaagcg
    28321 cccacaattg ggtctactac ccagacaggg aagcctaggc atgaaaacaa aatccagaca
    28381 caaatggctt gaaccgcata attatctatc gctgcaattg tgggaaacgc tgggcagtgc
    28441 cttgttcaac ctgctcatct ttttcatgtt gttcgtttat ctttttccaa tgcttttcat
    28501 ggttgccact tcgctcatgg aagggattca actccgtgac cgcaatgccc ccgcctaccc
    28561 atcgcaacag gtccgctttg aatatcaggg cgaggaatat ctggtgtaca acgttcccat
    28621 caacggcgga gtgcgtcagc tggctctggt ggagccaaaa caaacctcca gcaaattcat
    28681 cgacccgcaa aaccccgaag atggtctcat tccctggaag ggcagttggc gccagttgaa
    28741 aggcgtctat cgttttcacg taacattcga taatttcaaa attctgttca gttccctgcg
    28801 tatccctgag atggtaaaaa acaccttcat aatgaccatc atcagcgaga tcggggttct
    28861 gctctcatcg atcgtagtgg cgtatggttt ttcgcgcttc ccattgcctg gagggaattt
    28921 tctattttat gtcctgatcg ccaccatatt aattcccgag aaggtcacgc tcatcccaac
    28981 ctatttcttt tttgcgcgcg ttctcaaatg gcagggcacc tggctgccca ttttgattcc
    29041 gttcttcttt gggaatgccg tttacatctt tttgctgaga caaaacttca agagcattcc
    29101 aaaagacctc gatgaagctg ccatgcttga cggcgctggt ccattgcgga ttttgtttca
    29161 ggtcatcctg ccgcaatcgt ggcctaccgt cctcaccgtg tcgctgctgc atttctacta
    29221 catgtggaac gaaacccgac aggcagccct gtacctcagc acccgcagag accttgctcc
    29281 catttccttt ggggtgcaaa gttttcaaag cctcgccccc attcaaaacc agcttcaagc
    29341 gggcgcgctc attttgatga tcgtccccgt cattgtgttg atcatttcac aacgttactt
    29401 catgcgcgac atggtggtga caggaatgga aaagtagacc catgttcaaa aatccgaatc
    29461 accgaaatat cttcggaatc tggctgggat gggcattgat catgctggcg tatcacaacc
    29521 tgctccctgc ccgcctcacc gttcaaccgc cagaccgcgc cctcgaatgg accgtcaccg
    29581 aaaccatgcc aggcagtcaa tatggaaaga tctacctgaa tgaacctttc ctcaaccgcc
    29641 acgtttcatg ggattcggaa tattatctcg ccatcgctgt cggcggctat gaagacccca
    29701 gccccacgcg gattcgctcc aacactggcg aaaccaccga aagcggagga ttctggccgt
    29761 tcgtcgtccc tccccaatct gccgaacgcc cgggcatatc attgagttac gccttcttcc
    29821 cgttctaccc catgatgatc cgcctgttcg cgcttccgct ttcgctgctt ggcatgaatg
    29881 ccatcgccac cgcctccctg gcaggagtga tcatttccat gctcggcaca ctcgccgcca
    29941 tgatcgcact ctacgaattc gcccgcaccg agctggatga aagcagcggt ctgcgcgccg
    30001 ccttttatct gcttattttt ccgagcggct tcttccttgc tgaaatctat actgaaggct
    30061 tgttcgtggg gttggcattc agcagtctgc tcctgcttcg gcgtggacat cgcggctggg
    30121 cggctcttgt ggctgtcctc gccaccttta cacgtgccgt gggcgtcacc ctcgccattc
    30181 cccttttcat ttcatggatc aaagatgcgg aatggctgga actcgacatg gaatggaagc
    30241 agatctacta ccgcggcctg ccctggcgca tccttgtcaa cgggttgatt gccactgctc
    30301 cgatcatcgc tttcttcctc tggcggatat cgtactacgg gctggccttc agccttgtgg
    30361 aagaagaatt cttcgggcgc ggcctgctct cgcttggata caccttcatt gcctggaaag
    30421 atggctttat ggagatcttc ggcaacaacc ccaacgcagc cgcctactat ttcgtggaat
    30481 ggcttggaat catcatcggg tttgctgcct gcattgcagg tttcaaaaga cacccagacc
    30541 tcgccatctt cggctttctt gtggtcttcc tctctttcac cagcggaccc gcgcagggca
    30601 tgtatcgcta tgttcttgcc gcaccgcctg tttttctctt tctcagcagg ctcggaaaaa
    30661 accccatctt tgaccgcatc tggaccattg ccagcatgct gctcatgagc atgatgtgca
    30721 tcatgtacat gttcgatatg tgggcaggct gaaattttca gcagtctcaa gaatcaaaac
    30781 atcccgcaga tttcatctgc gggatgtttt gattcatcaa gaacgatcag acattgacat
    30841 ttagagagaa caattgcggt tttgaattca ccacgcgctg aatctcgccg caagcttcca
    30901 tatccaattt tacgatggta aagtaccaca acactgagcc gtcaaaacca tcctgcagcc
    30961 gttcctccac acgatgccca agttgggtaa aggtcatggg gccgtgcatg cgcaagatct
    31021 caagtatgga ggcgtacaca atgtcatact tctccctgtt gagaaacaca tctttctgag
    31081 caggttcgag actcgtcaca aaagcaaaat cgccggtagc catgtgcttg tacgcctcgc
    31141 taatttgcag gattcaaact tgtgtaaaga ccaatggcat tcccggttgg gtcatgaaaa
    31201 accgcaaacc agcccatgcc cgggatctcc gtcttttgga aaatcacttt gccgcctaat
    31261 ttttcggcct ttttcaggtc ggcttcgata tcatcgctgt caatgtaaac cagaacctgc
    31321 cccgccgggt tctcagccga aacagcaggg aaaccgccgc cagagccatc gcctgcttcc
    31381 cacatggtgt agttcatttc aggcatgtgt tgaagcttcc agccaaacaa ttcctggtag
    31441 aacttgcctg caccttcaac attcgcagca ggaatctcaa catgaacaat atttcgcttg
    31501 gacatagcaa tattctcctt tggttaaagt gggtaattgt atgtttattt tagaacgcgt
    31561 gttctaaaat gtcaagcgcc atatttgggg acttttcgaa tgtgcccccc agatgtactt
    31621 tggtcgttta tcgaccaaaa cgcgcaagga tgcgcaggaa aatttccctg tcatcaggca
    31681 tgatctctgc catggcaaag cccacgtgat acagattcgg ctcgatctca tcaattcggc
    31741 accattttga gcgggcaata aaaataatga acggcctgtc tgccagttcg ggggtgagct
    31801 ctacacgcag gtaatagtct ttttcaaccg ggcagggagc tgttgtttca agctgcagac
    31861 caatggggct gacctcgacc atgtggccaa gaataccctg tgtgtcatca tctacaactc
    31921 gcaaataata atcaaacttt tttcgggggg tggttcgacg ctctggcatg gaaattctcc
    31981 ttcataaggc aatgattatt gcataatcct accattatgg atgaaggtga aaaatatacg
    32041 ttttatatta atttttcaag aaacatctgc agaatggcgg ggaaatttta cagggagaaa
    32101 atttccctcg aaagcgaggc aggttggggc ggagtgtgaa gcgtcggttt tattcgtatt
    32161 tcaaagcctg cacagggatc atcgtcgctg cacgcaaggc tggatacagg cctgaaagca
    32221 ttccaataat ggtggcgaag atgagggcga aaacgggcag ccatacgggg gtgtagaccg
    32281 ccacactggg aggcgggccg ccctgttcac tagcctgccc agccagatag atgatcgcaa
    32341 gcacgttgat gccttgtgca gccagccagc caatgatgac gccacccgcg cctccaataa
    32401 agccgatacc tgccgcctcc ccaagaaaaa tggacagcac atcgcggttg gttgcgccaa
    32461 ctgctttcat caggccgatc tcacgcgtac gctccaaaat agacatggcc attgtgttgg
    32521 caatgccaat ggctgcaacc agcaatgcaa tggtccctac gccgccaaag atgatctgca
    32581 aaacaaggaa gtagccattg atgctttcga ggtaggcttg cggggtaatg gcttgatacc
    32641 ccattgcggt gatctggtca gcaatatcga gggcctggtt ggggtcatcc accttgacaa
    32701 tgacctggtt gtagccagtc ttgttgtagt tgatgcgcac cccgtttgcc cattcgttca
    32761 aacttcgcac gaggtccagc ggaagataga tggaataatc cgcttcgccg cccgtttcgg
    32821 tgagaacacc cgctacgcgc aaactgatga tcttttgcga tacgttccct tcctgatccc
    32881 acttcatcac gaccatttga atttgctcgt tgtacagatt gggtggaagg ggcggctcct
    32941 gcccggggcg caggtttgga tcgtagaagt tgttgggcgt catggcgccg atcaccacag
    33001 aacctcttga aaggatggtt gtgccttcgc tggcctccag accaatattg gcaaggtcat
    33061 ccgtatctat gccaatgaag cccatgcctg cttccaattt acgataggag atgatggtgg
    33121 aagcattgag gtaatcacgt gggatcaccg tatggacgcc ggggatggcg cgaaactgtt
    33181 cgaggaccga gtttgtaatg agagctggtt catccaaccc tccacctccc cccccgccgc
    33241 cgcccatcat cggggcgcca tatccgccgc cataaaatac accgtaggca ggcatgacat
    33301 caatcaaggt caggtcaccg atgccgtaga gttggtcggt ggcactcttt tggaggccaa
    33361 cagccaatga aacaaggatg accaccgccg ccgtaccgat gaccacgccg atggcggtca
    33421 acgctacgcg agctttacgg cggccaagat tgccgaagat caggcggagc aggtcgagaa
    33481 atttcatatc cgattaaccg cctttgccgc catttgcagg catgggcagg ggctcacctt
    33541 ccagggcggg gccttctgtg gggaaattgt ttgcaggtga agcggaatcc aaaccgagca
    33601 ggccaaggat tccacgccaa atcttttgga ggagggtttc ttcttccaca ggaataaatt
    33661 cgccgcctcc gccgccaagt gagggatcaa gcgaagggtc aagggcgggg tctacaaagg
    33721 cttcctccac ggtcacttgc aatttgcggg tgatcgtgcg cggttggttg aaatcgtcgg
    33781 tgtattcgat ggtgactgtc aattccatcg ggccagcgac atcggggatc agggtcgtat
    33841 caaaggtgaa gtaaccgccc gggtccaacg acccgaccaa ggttgtggca ggatcgacca
    33901 ttccatttgt cgtttcaatt ttcatatttc ccagcacaga agcacgcttt ccgaggctga
    33961 cgatctgaat cggcaaagcc ccgggctgcc ccgcaaaaaa cggatcaggc gggcggtaga
    34021 aactcacatc cacatttgga aggctgtaca ccaacaacgt gatgacctga tcgtcattaa
    34081 ccacttcgcc cttgttgtta atgtatgaaa acgagatctt catcgggtag gcgcccgggt
    34141 tggtggatac attaacgatc aaactttgga agacctgcaa ctgtccgcct gcgggaagat
    34201 cgccaagcga ttgaatgttt gaagaattga caggcgcaaa gttggtaaat tcaccgccgc
    34261 tgcccgaaac accaccaggc tggggagtgc cgctgctatc accggaggaa ccgcccccaa
    34321 cgatcatggt gacgttcttc gcactggtgt tccccatatt ttggactgtc agcccaagct
    34381 ggaactgctc tcccggctgc aacggatcca ccgtggaccc gtaactggtg atgaccaatt
    34441 gggcagtgtt cacccctgtg ggggttgccg tggggtacgg cacattggaa cctcccacgg
    34501 ggatgctgag cgtaaattta tcggtgtact cggtgccggc actgtcataa taggtcagcg
    34561 ttacatctgt ggtaacgaca gatttcccat aaatagagtc gagggcgatg aatggctggg
    34621 tggcgtcatt atggtcaccc gcacggatcg tgcccaaaaa gatgacaccg cccgttttgg
    34681 tggcaatcaa atcagcagac gtgaaaacag cctgaacatt gtaggcagcc acagtgccag
    34741 cattgccaaa atcaatgtta agtttgaaat catctcctga acggacacgc tgcacattga
    34801 cctcatagga accaatgacc atttgtggac ggtcaaaagg cgccgaggtc ggtgtgggta
    34861 aaggggatgt atcggctacg gtcagtccgt tggggtactc aaaggtggtc gcatctgggt
    34921 tggtgactat gaccgtataa gaacccgcag gcagccccgg gcgcaccaca gcccgcaatt
    34981 ggttgggacc atcgaacgag gtggtgaggt caccgtaatt tttcaacgaa accaccgcac
    35041 cagattcaaa ccccgacccc gtgatcgtaa cggtgctggg agcatcattg aacacaagat
    35101 tggggtcgat cgagctgatg atcgggtctg cgtaaacagg cgtagcccaa cttgccaaaa
    35161 aagcggctgc cagaagaatg gaaacaaaat atttactttg gaacttcatg tactctccca
    35221 tgattcaatt tacgcggaag gtgaatctga caaagtctgc atcatatcca tcgaagcaga
    35281 ttgatattcc tcttccgtca ccacatgacc atccaacagg aatattttgt gtgtagcaaa
    35341 acgggtcatg cgcgggtcat gcgtcaccac cagtacggta cgccctgaac gatgcagatc
    35401 agaaagcaac tgcatcacag tcaccccgct ggctgtatca agatttcctg tcggctcatc
    35461 ggcgagaacc aggctcggat cattcaccag ggcccttgcc acagccacac gctgctgctg
    35521 accacccgaa agctcggtgg gacgatgatg actgcgttcc cccaagccca cctgttccag
    35581 cagctgacgc gagcggctat tccgctctga aacgggaatg cccgaaaact gcatgggaaa
    35641 agatacattt tccaatgcgg tcatgctggg aattaagtta tacgactgaa agataaatcc
    35701 catggtacgg cggcggaaaa gcgcgagcgt gttttcatcc atttggtcga ggcgctcacc
    35761 actgatcgtg atctcgcccg aagtggaacg gtccaacccg ccaagcaggt acaacaaact
    35821 gctcttaccc gaccccgaag gccccatcac caccgtaaag gtgtgcggct caatatcaag
    35881 gctaacccca tccaatgcgc ggacggtcgt cccacccatc tggtaatgtt ttttcagatc
    35941 acgaatttga atgagaggcg aggtcatagg caaaaatgga attagaacgc tgccccgctg
    36001 ctgaaactgg aaaccaatgt ccctgcccca gccaatgcaa gaagaatgac aaccagaatg
    36061 acgatcacta caaggatggc cttattcctt gtaaggccat ctgctgtgcc aaacccaaga
    36121 attaagagga tggcattcca gataacgaaa atatccaccc gtgaaagcat cttgctcagg
    36181 aacacagccc cacccgcaaa ccccgataac cccgggctga caatggtatg cccaacgatc
    36241 agcatgaaaa ccacccgcag cacatcacgc agcaaaaatg gcaaaccacc ccagccaaca
    36301 acattcaggg cactgcccat cgtaccgcgc ccacccatca aagtggagcc gaggtggagc
    36361 agcccgctca aaatcaacca tcccagccac aaaccagcca gcgctgaaag cagcggaatg
    36421 atgtacacaa aagtcgctcc ctgtgtggcc tgctgggcct gcatgaagtt attctgcata
    36481 tcaggggtcc aatactgcca atcgggaggc agttggatct cccccatcgc tgctgcacgc
    36541 gctttcaaaa atccactcac aatgacgacc agggaagacg aaatgctcaa cgccagcatc
    36601 ggagttagcc atgaagatac ggtctctcct gcaatttctg caaaggcgcg tttgggatga
    36661 agcaaaactt cgagaatgcg cgcaaaattg aatcggcgcg ttggaagagg aggatttgat
    36721 tcagtcatag tgtttttttt gtagtttgga gttgagaaaa ttctatcatg ctttccaaaa
    36781 agggattctc acccgaatca acgaaaaacg actggaaatg gttcccagtc gttttgacta
    36841 ccaaacatgt ctattttttc aaaatcattt cggacgataa agcaatgtcg ctgttgtgat
    36901 ggtggtaagt gtaggctttg caatataaac tgattgcgag gtcctgatca caatataagg
    36961 cggcgaaccc aatttgtact tcatcaggaa ctcaaccgtg taccactgat tgacaggaac
    37021 gttgttgaaa atgaaggtgc agccgttgct cgcaggcgga tttgtaaagg tagccacgta
    37081 actgccattc ctgttataca gggttgcctt ggtacagtcg taggtatctg cattcggcag
    37141 tgaccgattg ggcttaacgg gaactttcac gatcgtggtt gtactatcgg gataagccaa
    37201 agatggggtg gtcactgtgc cagccaaaag cgccacaagg accagggaaa caaagagcaa
    37261 acgcaggtgc ttcataacaa cctccttgtt gtgtacgaaa agtataaacc catcaagagg
    37321 tacaaacaac ctacattttt gaaaatgcaa cgaaactaaa ttccttcgaa caagtccgtt
    37381 ttgcgctttg aattcccagc cacaggcgga taggcctgca tataatcttc gttctcgcca
    37441 aacatgcgcg ttacaaagcc catcaatccg cgccgcatct gacctccgcc ctgcgaggca
    37501 tgacacgcgc tcgccttttg cttcgcctca gccgcagaag ccacattcaa acggacatgg
    37561 atcgggaagg tcacttccgc cagttccttg atattgatat cgccgttgcg gccaaatttg
    37621 gtcgggtctt ttccaaagag cggcatcaaa cgcacagccg ccttcagcag gcctttcgga
    37681 aaaacctgaa agtacaaggc cttcggcttg aaagccgcgc cagcctcagg gtggaaagat
    37741 gcgtcgtctg ccttttcgaa ggcaagcacc gtcgccttgt gaatatgaat gtggtcagga
    37801 tgtttgtatc cgccgatcgg gtcaaaggtc aaaaccacct cgggcttgag tgtgcggatg
    37861 tgtttcacca cacgccccgc tacctcatcg atcggcgcat tgatctgtgc atcggggtgc
    37921 ttgttatctt ccgtgccagg catacccgaa tcacgataac cgaggaaaaa aatttccttc
    37981 aaaccaagtt ctttgcctgc gcgcatcaat tcatcggtgc gcatctcggc ggtatctttg
    38041 aaacccttca aatgctccgc atccacggtg ccaacttcgc cccgcgtcgc gcagacataa
    38101 tacacatcat agccacggct ggcatacaag gccagtgtgc cgcccaaacc aaaagactca
    38161 tcatcagggt gagccagaac cgcaagtaaa gtttttgtca tctcattttc ctctcgtgca
    38221 tagacgtcca ccagacgcca aagtttacct tagtatgata aaattttgtc ctatcaagga
    38281 gcacccagcg aatgacggat attctgtaaa caaccagttt tttcagggca ccaacccgct
    38341 gttagcagtg gcgacatcca atcgcccact gtggattccg atgacatcga tctattggaa
    38401 aagcatcagc cgaggcatgc cctcggtttt ctttttaact acaggaacgt cattatgctg
    38461 agtattcaca acctatccaa aagctacggt atccaacccg ttttacaaaa tatcaacttc
    38521 aacatcagcg ctggcgagcg catcggactg atcggcccga acggatcggg caagaccacc
    38581 ctcatgcgca tcctcgcaaa actcgaacaa cctgacgcag ggcgcgggga tgtcgcttcg
    38641 acacgcccca acctgcggat tggatatctc gcccaaggca tggacctcga ccccaggcag
    38701 acgcttcata ctgcgctcgg gcttgatcct gccacccaaa cagatcccgc cgctgaaata
    38761 gagtcgctcg ccctcgcctt atctcaaaac ccaaatgact ctgccctgca gacacaatac
    38821 gaccacgcac ttacaaaact ttcagcctta aacctgcagc ctgaaaccat cctcgccccg
    38881 ctcggccttg accatctccc cgccgataca cccatcgccc atctcagcgg tggacaaaag
    38941 acccgcctca tgctcgcgcg cgtgttactt gaagaaccac aattgctgct gctggatgaa
    39001 cccaccaacc acctcgatat cgaaatgctc gaatggctcg aagaatggct gaatcaattt
    39061 caaggcgcgg cattgatcgt ctctcatgac cgcgtatttc tcgataacac agtcacctcc
    39121 atccttgaat tgaatccgat cacacacggc at
//
LOCUS       DAOQJC010000021        38511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_448_length_38511_cov_9.681870, whole genome shotgun sequence.
ACCESSION   DAOQJC010000021 DAOQJC010000000
VERSION     DAOQJC010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 38511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38511
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_448_length_38511_cov_9.681870"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1925
                     /locus_tag="PLE39_04805"
     CDS             201..1925
                     /locus_tag="PLE39_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNE67677.1"
                     /translation="MPRRSLARLYKDEFSGYSFAVFQKDLIAGLSVAAVALPLALAFG
                     AGSGAGAAAGLITSIIGGLVIGALGGAPYQISGAKAVTSVVLIVLVNRYGLEGMWFAS
                     LFSGLLMLTIGLLKLGRYIAFIPAPVIRGFTLGIALIIIIRQMDNLLGIHTPTTETAA
                     QKLIGYFNEDFIINPQAILLGLVVIVTMLLWPKKWNLYFPSTLAGLILATLLNQTLGW
                     SVTTIQSIPRTLILEQHLDLTKVPWTNLSDFIAPALTLTALGSIEAFLCGAVGSNMTG
                     IRLQVNQQLIGQGLGNITLPFFGAIPTTSAMIRTRVAIQSGARTRLVSIIHSLVLLIV
                     MFLFAPFLEQIPLAALAGLLMVVAIRTNEWDSIKRIFARRFKTDMIAFSIAMIATVVL
                     SLTDAILLGTFLAGAVFLNKIASIDISLQDVDVDRLKQRGIETAGKCKHIRVAFLTGP
                     LFFAATGQFEEAFANLKDTHALILSMRGVPLIDTEGLEEIHRLHERLRKQGGTLMFAG
                     IHDNARAMMERDGLVEAIGAENFFWSSDQAIVEAERRDCAFCAEEIAESPENAAAEDP
                     ITNNSSWR"
     gene            1947..3626
                     /locus_tag="PLE39_04810"
     CDS             1947..3626
                     /locus_tag="PLE39_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNE67678.1"
                     /translation="MPRRSLVRLYRDEFANYSPAKFQQDLLAGLTVAAVALPLALAFG
                     VASGASAAAGLVTAVLAGFIMGLLGGAPYQISGPTGAMSAVLIVLVTRYGLEGIWVAG
                     LFSGFLLLLIGILRLGRFIAFIPAPVISGFTSGIALIIFIGQIDNFFGIKTAAAETAA
                     QKFIGYFTTPFSFDWHSVCLALVVIGAMLFWPARWNARFPASLLGIILATMLNWILSW
                     PVSMIGAIPQTLFLEQRLSFASIPWGNLSDFIAPTLTITALGAVESLLCGAVGSNMTG
                     VRLQANQELIAQGIGNMVIPFFGGVPATAAIARSSVGIKSGGQTRLVSVIHAVGILLS
                     MFLLTVFMERIPLAALAGVLMVTAVRMNEWDAIKFIFGRRFKTDMIAFTITMFATIVL
                     DLTQAILIGSFLAGAVFLNKIASIDITVQDVDIKRLQEKGIKTDGRCRHVRVAFLTGP
                     LFFAATGQFNEAFSNLKETHALILSMRGVSLIDTAGIEAIHRLHERLHKQGGTLMFAG
                     LHDNARQMMERGGLVETIGERNFFWSSDQAIVEAERRGCKFCRTRHKFHTV"
     gene            3773..4594
                     /locus_tag="PLE39_04815"
     CDS             3773..4594
                     /locus_tag="PLE39_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNE67679.1"
                     /translation="MQLTDTHCHLDYHKFNPDRAEVIQRATDAGLIRLLVPGLHHRSS
                     KEAVKLAESNPSVYAAVGFHPTDLDEFTEKTFEKVKDLARHPKVVAIGEIGIDYYWVK
                     EMEKRAIQREVLKRQLQFAQEVGKPVVIHMREEGDAWTGDASVDLLAILSEWTSGLSG
                     TLKERPGVLHSFNGDLETAQKALDLNFFLGITGPVTYKTADKKREIIGQIPLDRILIE
                     TDSPFLSPVPHRGERNEPAYVRHIADKIAEIHLKSPAEIASITTANAARLFAWGD"
     gene            4596..5561
                     /locus_tag="PLE39_04820"
     CDS             4596..5561
                     /locus_tag="PLE39_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNE67680.1"
                     /translation="MNAVTFLSPVTQEIKLVEERMRMQADESHPDLRAALEHLLVAGG
                     KRVRPTLGLLVGNMLNAPLDKLVTLGAAVELLHTATLVHDDLIDGALLRRGMPTLNAR
                     WSPPATVLTGDFLFARAAKLAAEADHLALMKLFSETLAIIVNGELTQMFTSRGLINRE
                     NYYKRIYAKTASLFEMTSRASAMVSPVDDGIIESMRDFGYQVGMAFQIVDDILDFTGE
                     QAAVGKPLGSDLHNGLVTLPAIYYAEANPDDADVLSLPNGGWTNNENMTRLVDNIRAS
                     DSSRLAMREAERHVDRALTILTTFENCAEREALENLAKYIVDRKV"
     gene            5671..6180
                     /locus_tag="PLE39_04825"
     CDS             5671..6180
                     /locus_tag="PLE39_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNE67681.1"
                     /translation="MPSPKKPFRINVGFIIYEEVGNNYDFPFEFNKIVLGDDLELRNF
                     EGIVNVGKTPQGLVVQADFSAETTLGCVRCLSDFDHELDWSFTELYAFDKRSETESAL
                     ILPEDAHIDLGELLREYALLEVPISPICKPDCKGLCTECGQNLNEKDCGHHEDLSDSP
                     FAKLKDLLK"
     gene            6250..7473
                     /locus_tag="PLE39_04830"
     CDS             6250..7473
                     /locus_tag="PLE39_04830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNE67682.1"
                     /translation="MNSSIQKTLGFIVTLVLIGGMIFLRARLSYNTFDFQSSNFTFFW
                     LSGRMLIEGENPYDEKQYLAGHETYGIKWQPNRIFPYPLPLAIFFIPLGFLSLPAAYI
                     TWQVITLLIVATTIFLLLAHWQESAQRLLLVPVFAAMFFFGPMYLTLHAGSVGAIALL
                     ALLAAILLLEKDKSLLAGIVLSLTVLKPPQGVPILFLAGIWFLARRDWKAIYGMVIGG
                     IALLLIGMIQDPMWVVKFRGASEAVMDRTQGVHSNVWAFAYLACGGTSPCWTLLGAAL
                     SLLLLGAAGFFLWKNHPQGAAPAKWSAWEAFNLIIPISFVSTIYLWAYDQIPYLIPIV
                     WIIGVLVEKSRNFIYAFLFLIVLVLYSLFALVQQAGTDKDLWSLGTTLIVLGSLWLAS
                     RMKQKPPIDKPSSTA"
     gene            7715..9511
                     /gene="metG"
                     /locus_tag="PLE39_04835"
     CDS             7715..9511
                     /gene="metG"
                     /locus_tag="PLE39_04835"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNE67683.1"
                     /translation="MPENILIAVAWPYANAEIHVGNLTGSHLPGDIAARYHRLKGNKV
                     LMITGTDSHGTPVTMSADKLGKPVEEVYKSYHEGFIDLFQKIGISYDLYTTTHSENHF
                     KVSQTIFLALQKNGYLFKKTEPQWFSPGLNRFLPDRYIEGTCYICGTEGARSDQCDTC
                     GNVLDPMKLVNPKSKVEGDNSSPELRDTEHFYIDLSKLEPDVVKYLKDRADHMRDTVL
                     GESLRKVEAEGLKPRAITRDLDWGIPVPVDEAGWDTKKLYVWFEAVIGYLSAPIEWSQ
                     VSGNGEAWRDWWTNPNAKQFHFIGKDNIFFHTSLWPAELMGVGSAFMELFAGEEVPLI
                     LPYDVPANQFMNLEGKKISGSRSWAVWGRDALTRFDPDAIRYYLTINMPETKDSDWDW
                     AEFVARNNNELVATWGNLANRVLAFCYKNWEGHVPDVDLNTLRPADLELLAAIENGFN
                     TVGTELEAVHLRSAVGEAMKLATVVNQYLDVNAPWSAIKVDKVEAGKTIYTALKAIDS
                     LKVIFAPFLPFTSEKLHGFFNYETPLFGEQYTQEVSDSLGTHTVLRYKPVEGMQWKPS
                     DLKPGAKLNQPGPLFKKLDEIVSVEERARLGK"
     gene            complement(9569..11413)
                     /locus_tag="PLE39_04840"
     CDS             complement(9569..11413)
                     /locus_tag="PLE39_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNE67684.1"
                     /translation="MKKMIRPFISSILGLLLLASCSFGSSDTQAAVAAQATSALPAVV
                     TLELTVQADTSVTYNTVGQVIKLTQNVRNSGSVSAPGPMIVTGAICPDMTTIGNLDAN
                     FDANETITCNSSYTITQADLDKGSVTITTMATVNNVNSNQVTTSIATVPPVVLKLTKT
                     AAPTTYSQAGQTITYSYVITNVGAATLGPAQFTVTDAAFAAPFNCGEATATLASNATV
                     TCTATYTITQADMGVASISTNATATGGGAPVSQAVGATVTKSDAAPQPSGGQRTHTVV
                     EGEWLWQIARCYGVDPNKLAADNKDKIPDPSKIKPGVILTVNNPGGYSKFYGPPCITF
                     VSHTLQAGDTWATLGQKYSADATVLQLANPDTSLSAAGTVIKLRIPNYTMGTVTTPPA
                     TQSKTLSVTVAANPTAYNQTGQVITFTYTVKNSGTESLGPAQFTVSGGLLGSTPLNCG
                     AASSTLAAGATLTCTSTYTVTQEDMTVNSISHQVTASGGGAGPSQAASVTVNKSTALL
                     TLTIAANPLTYTQAGQIITFTYAIKNSGSTNLGPAQFTISGGLHGATPINCGDAAATI
                     LPGGTLTCTASYTITEADLAVPTLSHLVIAAGGGAAPSQPASITLTKQ"
     gene            11657..12802
                     /locus_tag="PLE39_04845"
     CDS             11657..12802
                     /locus_tag="PLE39_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE67685.1"
                     /translation="MDRVRALVLSGGGGRGAFHAGVYKYLMESGKPKVDSAHSAQWDP
                     QVIVGTSIGAVNGAAIAQGMPADELVRVWELLEEKDIQGLPPGMRGLARWVAHKIFGT
                     IMDANLPQVSPQDATSPMPKEFWPPLPITSKWWGERLVGRWINLLDTGPLRKTLHTHF
                     KFDPREIASSQKTLLIAATNVQTGERMMFSNRAIQRRDTGEARQDVVTSITADRILAS
                     CSIPLVYPWTFDPETNAHYWDGALVANTPIGVALDAMRDVPIDVPAEIVVVLMTPWWQ
                     SGDPIPYTARKLPESFGDAITWTLDWMLLASFRERLRLIKAFNDLAVRERAEGAGPYK
                     YREVKVIIAAPDDFLPAERIIDYDGDVSRDLIQQGYAAAKKAFEAAF"
     gene            complement(12799..14184)
                     /locus_tag="PLE39_04850"
     CDS             complement(12799..14184)
                     /locus_tag="PLE39_04850"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE67686.1"
                     /translation="MDINSPNFLFLFLPWFMLGYFFAPQRGKLIIGIAGSLLFYTWGN
                     LIYVPLMVTLTIFTYFLARGIEHWRGQKTAQILLWFGILINIALLVIFKLWTGIKYPL
                     GLSYVTFQVIAYLLEVYNKTSTSEKDLLAFSFYLLLFPKIPVGPITRYRQLREQIQNI
                     QVDPIEVAEGLRRYIRGFAKKALIADTLARVVTPIFKLASPTIAPSFAWLVIISYSLQ
                     LYFDFSGYTDMAIGLGRMMGFRFLENFNFPYISKSIGDFWRRWHISLSNWFRDFVFYP
                     LERRRLKWLGQPLNILIVFALTGLWHGLTRNFLIWGLIHGMALIFEATTLGKKLRTAW
                     APVQHVYALGVILVGWVFFRSPSPDFAFDFLRRLAGNMSGIQALPFETTSPLPFIEPT
                     FLLALAAGIIFSLPVGGWLQAKVDILTSTRPATRTPFQLAYDLGLFFLFLAALASTVS
                     AAFQPGIYGTF"
     regulatory      complement(14338..14434)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      complement(14526..14613)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(14735..15862)
                     /locus_tag="PLE39_04855"
     CDS             complement(14735..15862)
                     /locus_tag="PLE39_04855"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNE67687.1"
                     /translation="MKKQTLNIAAILVIFSLALAACGTQPATTPAPAEDVVTSNEVVA
                     EGRLEPIQGTNLSFQARGVVEEILVKAGDSVKQGDVLARLTNAGVAEAQVVIAQNAYD
                     TLLRNESGDRAKLWQAYMVAQEERGKAEQKWDDLNVDNIEDNIEDDKATVEDRKEDLQ
                     DALDEFEKYKDLDEDNSNRKAAEDDLERAQEDLNQAIRNLEEQIRERDEVRAKYDAAL
                     AVEAEAKYQYEISLDGPNADQLTLAKANLDSAKDTLSNYVIAAPFDGIVADVNIKAGE
                     QVTVDTRVLSIANFDSWVIETTDITELEVVKLSEGQPVTFVPDALSDVVLTGTVTEIS
                     EAFTQQGGDILYTVRIAVKETDPRLKWGMTVEVTFMEIVQE"
     gene            complement(15910..18342)
                     /locus_tag="PLE39_04860"
     CDS             complement(15910..18342)
                     /locus_tag="PLE39_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE67688.1"
                     /translation="MRPRWRKVLHDLWDNKARTLLVVFSIAVGVFSIGVIAGAYQIIS
                     NDMGISYAANNPSNIELRTANFDQDVLDNIRNMRGIEDAEARRVINFRARTIDSNAWV
                     SLDVIAFKSFKDNKVNLLKPVAGASEAKKDEILLDKRALEKFDFEIGDYLILELPDGT
                     SKQLRIAGIVQDSSSSAGDFLAPSLGFISMKTMPTLRQPDDLFNRAYITLSNGVDDSD
                     YIHEAGADIKDKLERNGTDVIRTRFSEKHKYPLADIITAILSILLALGILIVFLSSSL
                     IANTLDALLSQHLRHIGVIKLVGGRGRQVFAMYIALIMAFSFLALLVAVPSGGWGAYR
                     LAEFMAEKLRFDLLGYRIVPMALVIQVVIGLLVPLIAGIVPVVGGSRITVLRALSGDV
                     AGDENKPHKQTDRLNWFDWMLVRLTNMFARRGIHFPRPFIISIRNTFRRRGRLILTLF
                     TLTMGGAIFIAVFNVRVTLHNYMNDIGKYFRADVTLDFDKPYRLREVEQFALQIPNVR
                     SVEGWQFLSFELLHPDGAVAENVNVFAPPSDSTLVEPILVSGRWLEATDVRKLLISEK
                     VLDTYPGLQPGDPIFLKVNGHEETWEVIGLFKFVGQEGVFAYIPYEYAVQITNLPNQS
                     FSFRVVTLQHDRISQDQMAEQLDRFFRDEGFKVRKAESGLASLDSALESLDILVAFLL
                     IMAVLTAIVGAMGLTGTMGMNVLERTREIGIMRAIGADDRAVMRTIIAEGFVIGTISF
                     VLAIGVSIPFTYGLSYIVSLAVFETPIDVVFTYLGYAIWFGLVLFLSMLGSILPARNA
                     ARLTIREVLAYE"
     gene            complement(18397..20466)
                     /locus_tag="PLE39_04865"
     CDS             complement(18397..20466)
                     /locus_tag="PLE39_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67689.1"
                     /translation="MTNDIHRHATLLKAAARAAKNITTILDPYDLLQRTVDIICDEFG
                     FYYAGVFFLDDAKQYAVLKAGRGEAGREMIHAGHRLAVGGNSMIGACIANRQGRIALD
                     VGAEAVFFENPHLPKTRSEMALPLVVGEDVIGALTVQSTEEAAFHDEDIAALQTMADQ
                     LAIAIQNSNLHRQAERRSRLLKAANRVGKEVTSILDLEKLLPQTVNIICESYGLYYAG
                     VFLVDEAGEYAVLRAGYGKAGKAMVADGHKLKIGTDSMIGACIAMSEARIALDVGEER
                     VHFKNPHLPHTRSEMALPLIYGGKVLGAVTIQSSEERAFSEDDITTLITMAEHLAVAI
                     NNARLIDELKEAHDEILRNKVFEALTTASTEAIHWIGNKTLPISLTVSRLQQEIAEGQ
                     VDVDSLREDLEMIAESAAQIIQVKEQLIGAVREQKPRPVLFADVVQTAAHQRDISSDM
                     LKVKIDPAAAYVIADSTQLVRAVGNILQNAAEAGAKSIRITAHETEERGFLEIMVDDN
                     GSGMDRDVMEKAWSPFFTTRGVDHHGLGLPAAMHVISQAQGRIAIVSEQGKGSTVAMY
                     LPKGILADEPLKTGSVKNLLLIDDNDDWANHFSNMLKGSNVKLTQTTDLKKLPAADLI
                     LVDENITSLPLAEVLDAIVKAGLAAKTVVLTSAINPERVTQFMHNGMKDVNVKPYSAG
                     EVSELLK"
     gene            complement(20521..20910)
                     /locus_tag="PLE39_04870"
     CDS             complement(20521..20910)
                     /locus_tag="PLE39_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE67690.1"
                     /translation="MTTVLLVDDEKLILRSLEKTLLRAGFDVITASDCKSGSETFAQN
                     MGEINIAVLDLNMPGFDGMPKTGAGLDLLADLKKQKASLPVIILTAYDEVTKAKDAVS
                     GGASAFFVKGREQGLVDLIQKTLQATG"
     gene            complement(20991..22889)
                     /locus_tag="PLE39_04875"
     CDS             complement(20991..22889)
                     /locus_tag="PLE39_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE67691.1"
                     /translation="MPDPRRTPQILYIEDSDETRSLVRRLLSKKYVVLEASDPLDGFQ
                     LAEETHPSLILLDNSLPHMSGIEAATRLSKMLPNTPIVVVSGDLNPGVRERALAAGAV
                     GFISKPIDADFVDQVDAYLNGRVEKLDNAEEHLQAYQQELVERLEGNIRQLTNTVEKN
                     KHLLQQNERMFSMLERRHKLLETAARVGQMVTSILDLDDLLKHTVNIICSEFSFYYSG
                     IFLVSEDGHWAVLRAGYAAAGRKMMAENYRLPVDEKSMIGKSIIQQQAQIALDVAGEE
                     SRFKNPFLPNTRSEMALPLIVKSRSLGGLTVQSDELNAFTEEDIAALQTMADQIAIAI
                     NNAQLMFKLEDATAELVRSKTFEAIATATGEAIHWVGNKAAPIPGSIKRAREDIRYIL
                     ALAAEYAPRDLDAKNVSLMEAVETIREEAQAMGIDLIQILAEMSAMKPNRLRALVSVE
                     SLMEDLDIAENSAETILSIKEGLIGPARQRNDAPVSLRDMIADTVANMGLPDGVVEMD
                     WADNVPNAFVDARQVEQVFNNLIKNAWEAMSQTPEPHIHVKGQRDSDPGFVLVTVKDN
                     GPGIPKEIQEKIWVSFFTTKGGSGGTGLGLSAVMQIVNQHGGSISLESEAGKGATFSV
                     RLPVEKKT"
     gene            complement(22922..24061)
                     /locus_tag="PLE39_04880"
     CDS             complement(22922..24061)
                     /locus_tag="PLE39_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE67692.1"
                     /translation="MNKTPLRTQPKSGPAQVADFAPESMIELRSVVKKFSNAAGEFTV
                     LKGVDLTINRGEFVSIVGKSGSGKSTLLNMITGIDHPTDGLVVVNGTDIYTGVSESQR
                     SKWRGKNLGIVFQFFQLLPMLTLLENVMLPMDYVDMYDFDERPTRAMELLKLVGLEKF
                     ANKLPVLVSTGQQQLAAIARAMACDPPLLVADEPTGNLDSRSADTIIRLFELFVSQGK
                     TIVMVTHDPSLTSRTNRNIIISDGELINETISRALPQLRHRHMLEFTRLAEERMVSPN
                     ETILQRGQAVEHFFMIRKGEVDLHGQTALRLRPRQFFGEIELLQGGGALTEVRAGSEP
                     VELLTIPRADFTRVIQESPITAEVLLKVAQEKMEALKEDKLAAKP"
     gene            complement(24058..24795)
                     /locus_tag="PLE39_04885"
     CDS             complement(24058..24795)
                     /locus_tag="PLE39_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE67693.1"
                     /translation="MPNPLIELNNVSKTYSTAAGDFVALKEVNLKVEMGEFLGIVGKS
                     GAGKSTLLNMINGVDQLTSGEVIVSSNGKNVSIHDMNEDALALWRGKNMGVVYQSFQL
                     LPMLTLIENITLPMDLSNNFHPRRSKERAMELLRLVELEEHAYKLPAFISGGQQQRVA
                     IARALANDPPILVADEPTGSLDSITADHIFDVFEHLVSEQDKTIIMVTHDMGLASRFS
                     RSLTITDGEIATEDPEPQLDKTFRRRR"
     gene            complement(24886..25734)
                     /locus_tag="PLE39_04890"
     CDS             complement(24886..25734)
                     /locus_tag="PLE39_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE67694.1"
                     /translation="MKSVVVTGVSTGIGWGVTKVLIQNGFRVFGSVRKTQDAERLKAE
                     FGDAFSPLIFDVTDEEKVHAAARQVREALNGETLFGLVNNAGIAIPAPMAHMPIAEFR
                     SQIEINLTGQLIVTQAFLPLLGMDHSLKGQPGRLINMSSTSGKRGAPFVGAYVTSKHA
                     LEGLSETLRIELMLYGIDVIIIGPGAVATPIWDKGEQADLTKYEHTEYIEAAKKFQRY
                     MVEDGRKGLPSEKIGEVVLHALTTPKPKVRYPVVPNMLKNWILPMLLPKRVFDNLIAK
                     GLGFRK"
     gene            26052..27170
                     /locus_tag="PLE39_04895"
     CDS             26052..27170
                     /locus_tag="PLE39_04895"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE67695.1"
                     /translation="MLRNFLADLKDVSPACREYFLKADSLLSALAIGISAAVIPPTAV
                     LDYLYYGFTEKFFATVLFEGVFVALCLLAMVAVRHAYTVAVYDWAVFIWTFMAAIGFS
                     IMGFDQPERVTENILVVVIFTTVIYTLVTNRLLFRLIPAWMMMISYLVIFGSDLPTAI
                     IPQQEIFFSASLLVVNIGGLLIVGSSNRHKQDDYQARQSEKEARQQLETLASIDPLTN
                     IFNRRHFMEIFNHELDRGKRYGARFSLVLMDLDHFKQVNDTYGHLAGDEALKAFVRII
                     QENKRKTDIFGRLGGEEFGLLMMETSGADALTTLQRFLSKARQEHIVMDDSTFHFTFS
                     AGITETLPKDRSIDEIIHRADEALYKAKESGRNHCVLV"
     gene            complement(27175..27927)
                     /locus_tag="PLE39_04900"
     CDS             complement(27175..27927)
                     /locus_tag="PLE39_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67696.1"
                     /translation="MNNFFGVNLFAQLRNRIKAVFGVFTASISVFACGAFMTFVLAPN
                     QAILAYKVSQIPLMTAQSVEAIAPGGDVLITGIINGDAPTPHLPEFIAYTEERWDVTV
                     SKNENGNDNTPSGSWTRVQTVVPLLTLDMDGTSVSIQASNTATLNGALHEKLVDGTGS
                     LQAVYEGQSLHDGAMRYKGLANGDLTTVFGKKASVGGVVPSQLFAGDRVAFEKYQRDL
                     ASSFLFMGITFMLCSPVVLVIGILGVLFGRKR"
     gene            28167..28610
                     /locus_tag="PLE39_04905"
     CDS             28167..28610
                     /locus_tag="PLE39_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE67697.1"
                     /translation="MNGEPVLVMLVEDNIDHAELVIRTLEDHRIANKIKHFMDGQTAL
                     DYLFRRGEYEDPKSSPRPHMILLDLRLPRVDGLEVLHQIKERDDLKNVPVIILTTSEA
                     EKDVARAYDNHVNSYLVKPVGFDEFSKLMNDLGFYWLGWNTHPRI"
     gene            28622..30016
                     /locus_tag="PLE39_04910"
     CDS             28622..30016
                     /locus_tag="PLE39_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE67698.1"
                     /translation="MKAQYMDDRINILLIEDEDPHAELIQRAFEDQDARFQIFRVRSL
                     TEARAQLAEKDPALIIADWRLPDGESMELLPDHHDPLSTPIILMTSYGNERIAVEALK
                     SGALDYVVKSPESMLDMPHLAERAIEQWGARAERAKMQKALLESEAQFRLLAENASDM
                     ISRLTTDGRVLYVSPACETILGYTPEELTGNSAFDIVHEDDRTFVRNLFKGNRYDATY
                     TIAYQALHKLGHYVWLESSAKAILDRKTNTISEIQIASRDITERKKSEKALQAAHEGL
                     QEAYQRTIEGWVRALDLRDRETEGHTQRVTELTVKVARRLGFSEEELTHIRRGALLHD
                     MGKMAIPDEILQKPGPLNETEWERMRRHPLYAYEMLSPISYLHPALEIPLCHHERWDG
                     SGYPRGLKGEEIPLVARFFAIVDVWDALCSDRPYRKKMPQQEVMNYLREKSGILFDPK
                     LVDVFLSVVESEEK"
     gene            30082..30180
                     /gene="ffs"
                     /locus_tag="PLE39_04915"
     ncRNA           30082..30180
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLE39_04915"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            30211..30570
                     /locus_tag="PLE39_04920"
     CDS             30211..30570
                     /locus_tag="PLE39_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67699.1"
                     /translation="MKSSNWIMIIAAAIIGIGIGLTYGWVINPVVYTDVTPDALRADY
                     RADYVLMVAEAYQSDQNPETAARRIAMIGSASPDQIVEESLDYATANNFSQNEILLLQ
                     GLLTAMQTYQPQPVNAP"
     gene            30567..31463
                     /locus_tag="PLE39_04925"
     CDS             30567..31463
                     /locus_tag="PLE39_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67700.1"
                     /translation="MRSSNRNSILYIVLALLTGLGLGLAYSWLLSPVTYVDATPAMLR
                     ADFKDQYRIVVAASYASSHDLARARARLQLLGDVDPVGQLSALAQNMVAGNASLEQVQ
                     PIAQLATDLQQGFSSSPVTDTPFVVVVNTPTEMDTPSAESLATETQIVLEEDTPIPTL
                     EFDQTPLAPIVTSTFVPRPTFTPVPVSGDVFNLVGQDTVCDPGLQPGLLQFIIMDSRR
                     RQVAGIEIIVSWSEKEDRFFTGFKPELGDGYADFVMQPDVIYSVHVVEGGAIVPNIAA
                     PTCTDPNGISYFGGLLLTFQQP"
     gene            31534..32073
                     /locus_tag="PLE39_04930"
     CDS             31534..32073
                     /locus_tag="PLE39_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67701.1"
                     /translation="MNTSYNNQKPGLLTAIIWMTLASGITNLFWGFVASATALGTIIG
                     VICLPITILPTILGVFEIIYAAKLLSAQPEPTKPSQTIAIFQILMILYGNVFSVVVGI
                     LALIFYNDQNVKDYFSRLNGVQVTTPDPMPVSPLRVEPEPLPEISPAEPVVPEIPALE
                     AEADPPVKPKRTRKSSTGK"
     regulatory      32182..32312
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            32542..32919
                     /locus_tag="PLE39_04935"
     CDS             32542..32919
                     /locus_tag="PLE39_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system H protein"
                     /protein_id="HNE67702.1"
                     /translation="MNAPSNLKYTKSDEWFDPASGAAGITDYAQNQLSDIVFVELLVD
                     EGETVEVGKPIASVESVKASAEIYAPASGTVSAVNKGLSEKPETLNSDPFGEGWMIKI
                     AGGSAGEVMDSAAYTTYCDGRAH"
     assembly_gap    32949..33048
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            33064..34413
                     /gene="gcvPA"
                     /locus_tag="PLE39_04940"
     CDS             33064..34413
                     /gene="gcvPA"
                     /locus_tag="PLE39_04940"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNE67703.1"
                     /translation="MTYIPISPKERDAMLKTVGVKSLDDLFEAIPQKHRFPELDLPPA
                     LTEMEAATLLSEMAQTNENVREHMISFLGAGAYNHYVPSVVDHMLRRGEFYTAYTPYQ
                     PEISQGTLQAIFEYQSLMTNLTGMEVSNASHYDGATATAEAVNLAYAQFRGKRKKIVM
                     SPTVHPQYREVIRTYSQGMGLEEAGDDVAADLEAGPEELMSLVDDKTMLVVVQYPDFF
                     GRIHDYTKLIEDAHAKGALVCIVANPTALLMLKTPASMGADIVVGEAQPFGIPLWFGG
                     PYLGFFTTKKSYVHKMAGRLIGETVDARGQRSYVLTLTAREQHIKRERATSNICTNQG
                     LLALASAVYMSTLGKTGLQQVANLCYQKAHYAASELSKIDGFGLCFTEPFFHEFVLCC
                     PKPASEVNDYLLDNGIFGGYDLGKDYPSLKNHLLVAVTEMNSKQEIDTLVEVLKEME"
     gene            34413..35900
                     /gene="gcvPB"
                     /locus_tag="PLE39_04945"
     CDS             34413..35900
                     /gene="gcvPB"
                     /locus_tag="PLE39_04945"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNE67704.1"
                     /translation="MATVVEPTIFELSSPGRLGVTMPASDVPETALPPNHLLRSDLPL
                     PELAEVDVVRHYMRLSKFNYSVDDGFYPLGSCTMKYNPKINEDTCRLPGFLFTHPLQP
                     IETVQGNLALMFEMQEWLKEISGFAGVTLQPAAGAHGEFTGVLMMAAYHKARNDTKRT
                     KMLIPDAAHGTNPASVGMLGMSVVTIPSDSRGNVDLKALEAACDDTVVGMMLTNPNTL
                     GMFDENIEQVVKMVKDCGGLMYGDGANLNALLGIVRPGDLGFDVMHFNLHKTFSVPHG
                     GGGPGSGPVGVSQRLVDYLPSPLVGIIEEGDDDLEPLYGFVTPKHSIGRMKAFHGHFG
                     GGLVRAYTYIAMHGPDGLKDVSQYAVLNANYLQARLRDTYKIPYDRICMHEFVMEGQF
                     EGSDIRALDISKRLMDYKFHPPTNYFPLIVHEALMIEPTETENKDTLDRFADALIAIA
                     EEAKSQPDLLHHAPHNTPFGRMDEVKAARELVLCCWLPENYETLK"
     gene            36041..36844
                     /locus_tag="PLE39_04950"
     CDS             36041..36844
                     /locus_tag="PLE39_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE67705.1"
                     /translation="MLNKLLFHRFPKAKSWLTRIQYELISTLDRDKNAIFMNFGYAPH
                     SHAYEPVALPKEEEIHRYPLQLYHHVARHIDWKNADALEVSSGRGGGAHFIMRHFKPR
                     SYTGVDFSTRAVDFCRRQYKLDGLKFHHGNAEALTFPDNSFDVVVNVEASLYYPNISK
                     FFEHVRRVLKPNGYFLYTDLRYEEKVAIWHAQLKNMGLKLIKEEDITDNVLKAMELDR
                     ARRVALVDRYVPAILRKQFYHFAGVSPDESETLPHLENRRYWYFVLQKA"
     gene            36944..37819
                     /locus_tag="PLE39_04955"
     CDS             36944..37819
                     /locus_tag="PLE39_04955"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNE67706.1"
                     /translation="MQPLSEDIRQKIILFQQTEITEYHIYKRLAKRIKSEGNAKIFDQ
                     IAEDELRHYNGWKEYTNEEVQPRWFFVWFYYLVSILFGFTFGVKLMEMGEEKAQANYE
                     AVSKVIPEATKYQDEEDKHEHQLIEMLDEERLQYAGSVVLGLNDALVELTGALAGLTL
                     ALQNVKLIALSGLITGIAASLSMAASEYLSTRSEETNKHPVRAAVYTGIAYIITVTLL
                     ILPYLLFENYYLDLVMALTTAVVIIAVFNYYISVAKGESFRERFVEMAGLSLSVAAFS
                     FVIGYFIRQWLGIEI"
     gene            37840..>38511
                     /locus_tag="PLE39_04960"
     CDS             37840..>38511
                     /locus_tag="PLE39_04960"
                     /inference="COORDINATES: protein motif:HMM:NF020318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 family protein"
                     /protein_id="HNE67707.1"
                     /translation="MKILSVDIGTGTQDIFLYDSNLDIENGFKLVLPSPTMMVHRRLK
                     QALHTRTPILLSGYQMGGGPSAWAIEEMARAGIPVYMTPDAATTLNDELDKVEKLGIK
                     IVSEDEVDGLRGKVESLELRDFDFGLISKTFADYGVSLSDLSAIAVAVFDHGNAPAGV
                     SDRQFRFDYLNERIRAKNALSSFAFLSSDIPRIMTRLQSVADSAHGLPCPLVVMDTAP
                     AAVLGA"
BASE COUNT         8896 a         9724 c        10162 g         9629 t
ORIGIN      
        1 gtgcgctctg tggttaaatc tttggttggc aagcccacga aattagccac tccctttttt
       61 cgttacggcc atccaaaaaa aatcaacagt gataaaataa ccgccaatca acagaggacg
      121 ccttttgggg gagcacctgt gccgagcaat tgcctcggcg tgagcctttg taaacaaatt
      181 ttcgtttcgg agaacaatcc atgcctcgaa gaagtctcgc tcgtctatat aaagacgagt
      241 tttcagggta ttcatttgcc gtttttcaaa aggatttaat agcaggcctg tctgttgcgg
      301 ctgtggcttt gccgttggcg ctggcctttg gggcgggttc gggcgcgggg gcagcggctg
      361 ggctgatcac gtccattatc ggtgggcttg tcatcggcgc tttgggcggg gcaccttacc
      421 agatcagcgg tgcaaaggct gtcaccagtg tggtgttgat cgtgctcgtc aaccgctacg
      481 gactcgaagg catgtggttt gccagtcttt tctctgggtt gctcatgctt accatcggtc
      541 tgcttaaact cggacgttac attgcattca ttcctgctcc cgttatccgc ggcttcactc
      601 tcggcattgc tcttattatt atcatccgcc aaatggacaa tctgcttggc atccacacgc
      661 ccactaccga aaccgccgcg caaaaattga ttggatattt caacgaagac ttcatcatca
      721 acccacaggc catcctgttg gggcttgtcg tcatcgtgac catgctgctc tggccgaaaa
      781 aatggaattt atattttccc tccacattgg caggactcat ccttgccacg cttcttaatc
      841 aaaccctggg ctggtcggtc acaaccattc agtccatccc ccgaaccttg atcctcgaac
      901 aacatctcga cctcaccaaa gtaccgtgga cgaatctttc cgatttcatt gctcccgccc
      961 tcaccctcac ggcgcttggc tccattgagg ctttcctgtg cggtgcggtc ggctcgaata
     1021 tgacgggtat ccgcctgcag gtgaatcagc agcttatagg gcagggattg ggcaatatca
     1081 ccctgccttt cttcggcgcc attcccacca cctcagccat gatccgcacc agggtggcga
     1141 tccaatcagg cgcacggact cgtttggtca gcatcattca ttcccttgtt ttgttgatcg
     1201 tcatgttcct cttcgcccca tttctcgagc agattccgct ggcggctttg gcggggttgc
     1261 ttatggtggt tgccatccgc accaatgagt gggattccat caaacgcatc tttgccaggc
     1321 gtttcaaaac cgatatgatc gccttcagca ttgccatgat cgccacggtt gtcctcagcc
     1381 tcaccgatgc gattttgctc ggaaccttcc ttgcgggcgc cgtttttctc aacaagatcg
     1441 ccagcattga tatcagcctg caggatgtgg atgttgacag gttgaagcag cgcggcattg
     1501 aaacggcggg caaatgcaaa catatccgtg tggcgtttct cactggcccc ttgttcttcg
     1561 cggccacagg tcaatttgaa gaagccttcg ccaacttgaa agacacccat gctttgatcc
     1621 tgtccatgcg cggtgttccg ctgatcgata cagaaggctt ggaggagatc caccgtctgc
     1681 acgaaagact gcgtaaacag ggcggcaccc tcatgtttgc aggcatccat gacaatgcgc
     1741 gtgcgatgat ggagcgtgat ggtttggtgg aagccatcgg cgctgaaaat ttcttttggt
     1801 ccagcgatca ggccatcgtt gaagcagaga ggcgtgattg cgcattttgt gcggaggaaa
     1861 ttgccgagtc gcctgaaaat gcggcggcag aagatcccat tacaaacaac agcagttgga
     1921 gataagattt cattggagaa ataaccatgc cgcgaagaag ccttgtgcga ttgtatcgag
     1981 atgagtttgc gaattattcg cctgccaaat ttcaacaaga cctgcttgct ggtttgaccg
     2041 ttgcggcagt tgcgcttcca ttggcgctgg cgtttggcgt tgcttcgggg gccagtgcag
     2101 cggcgggact ggtcactgcc gttcttgcag gtttcatcat ggggctgctc ggcggcgcac
     2161 cataccagat cagcggaccg acgggggcga tgtctgcagt attgatcgtg ctcgttacgc
     2221 gttatggctt ggaaggtatt tgggtggcgg ggttgttttc gggctttttg cttttattga
     2281 tcggcatttt gcgtttgggc cgattcattg cgttcatccc tgcaccagtc atcagcggat
     2341 tcacctccgg tattgcgctc atcatcttta tcggccagat cgataatttt tttggaatca
     2401 aaaccgcggc tgctgaaacc gccgcgcaaa agttcattgg atatttcacc acaccatttt
     2461 catttgattg gcattctgtt tgtcttgccc tggttgtgat cggggcgatg ttgttctggc
     2521 ctgccagatg gaatgcgcgt ttccctgcat ctttgctggg catcatcctg gcaacgatgc
     2581 ttaattggat cttgagctgg cccgtctcga tgatcggcgc cattccgcaa accttgttcc
     2641 tcgaacagcg tttatccttt gccagtattc cttggggaaa cctttctgat ttcattgctc
     2701 ccacgttgac gatcacagcg ctgggcgcag ttgaatcgtt gttgtgcggt gcggtcggct
     2761 cgaatatgac gggtgtgcgc ctgcaggcaa atcaggaact tattgcgcag ggcatcggca
     2821 atatggtgat cccgttcttt ggcggggtgc ccgccacggc ggcgatcgcg cgttcgagtg
     2881 tgggcatcaa atcaggcgga cagactcgtc tggtgagtgt gatccacgct gttgggattt
     2941 tgctatcgat gttcctgctc actgtattca tggagcggat tccgcttgcg gctttggctg
     3001 gtgtgttgat ggtcactgcc gtgcgcatga acgaatggga tgcgatcaaa ttcatctttg
     3061 gcaggcgctt caaaacggac atgatcgcct tcaccatcac catgttcgcc accattgtgc
     3121 tggacctgac ccaggccatc ctgattggtt ccttcctcgc tggggcggtc ttcctgaaca
     3181 agatcgccag cattgacatc actgttcagg atgtggatat taaacgtctg caagaaaagg
     3241 gtattaaaac cgatgggcgt tgcaggcatg tgcgcgtggc ctttctcacg ggtccgctct
     3301 tttttgcggc gacgggtcaa ttcaacgagg ctttttcaaa cctgaaggag actcatgccc
     3361 tcattctttc aatgcgcggc gtgtcgctga tcgatacagc aggcattgaa gccattcacc
     3421 gtttgcatga gcggctgcat aaacaaggcg gtacactcat gttcgcaggt ctgcacgaca
     3481 atgccaggca aatgatggag cgcggtgggt tggtggaaac catcggcgag cggaatttct
     3541 tctggtcgag cgatcaggcg attgtggaag cggagcggcg gggctgcaag ttctgccgaa
     3601 cgcgtcataa attccacacg gtataatcgt ttcaacccgt cagcaaaaaa gaccgtcgcc
     3661 agaaaatcgg cggcggtttt tgggtacaat cctgaaagat tgacaccctc caacacgtca
     3721 tcatgaaggc agcaaaacct gcaaccttca acctgaaacc tgaaatcccc tcatgcaact
     3781 caccgacaca cactgccacc tcgattatca taaattcaac cccgaccgcg cggaagtcat
     3841 tcaacgtgcg actgatgcgg ggctgattcg gctgctcgtg cccggactcc accacaggtc
     3901 cagcaaagag gcggtgaagt tggcagaatc caatcccagc gtgtatgcgg cggtcgggtt
     3961 tcatcccaca gatttggatg aattcacaga aaaaaccttc gaaaaagtga aagaccttgc
     4021 ccgccatccc aaagtggttg ccatcggtga gatcggaatt gactattact gggtgaagga
     4081 aatggaaaaa cgggcgatcc aacgcgaggt gctcaagcgg cagctgcaat tcgcgcagga
     4141 agtggggaag cctgtggtca ttcacatgcg cgaagaaggg gacgcctgga cgggcgatgc
     4201 ctcggttgac ctgcttgcga ttttgagcga gtggacgagt ggtttaagcg gcacactcaa
     4261 agaacgccct ggcgtactcc attcttttaa tggcgatctt gagaccgccc aaaaagccct
     4321 cgacctgaac tttttcctcg gaatcacagg cccggtgacc tataaaactg cggacaaaaa
     4381 gcgcgaaatt attggacaaa ttccccttga cagaatcctc atcgaaaccg actcgccttt
     4441 tctctccccg gtacctcatc gcggcgaacg taatgaacct gcctatgttc gtcatattgc
     4501 tgataaaatt gcagaaatcc atttaaaaag ccctgcagag atcgccagta tcacaaccgc
     4561 caacgcggca cggctctttg catggggaga ctgatttgaa cgcagtaact tttctctcac
     4621 cagtaacaca ggaaatcaaa ctcgttgaag aacgaatgcg gatgcaggcg gatgaaagcc
     4681 atcctgacct gcgtgccgca ttggagcatt tgcttgtggc aggtggaaaa cgagtccgac
     4741 ctacccttgg cctgctcgtt ggaaacatgc tgaacgcgcc cctcgacaaa ctcgtcaccc
     4801 tcggcgctgc cgttgaattg ttgcacactg ccaccctcgt ccacgatgac ctgatcgacg
     4861 gcgctttgct ccgcagaggc atgcccacct tgaatgcgcg ctggtcaccg cccgccactg
     4921 tattgacagg cgacttcctc tttgcacgcg ccgccaaact tgccgccgaa gccgatcact
     4981 tggcgttgat gaaattattt tccgaaacac tggcgatcat tgtcaacggt gagttgacgc
     5041 aaatgttcac ctcacgcggg ttgatcaacc gcgagaatta ttacaaacgc atttatgcca
     5101 agaccgcatc gctgttcgaa atgacctccc gcgcctcggc catggtcagc cctgtggatg
     5161 acggcatcat cgaatccatg cgcgattttg gctatcaagt tggcatggct tttcagatcg
     5221 tggatgacat tctcgatttc actggggaac aggctgccgt ggggaaaccc ctcggctcag
     5281 accttcacaa tgggctggtg acactcccag ccatttacta cgctgaagcc aatccagatg
     5341 atgcggatgt cctctcgctg cccaatggcg gctggaccaa taacgagaac atgacccgcc
     5401 ttgtggataa tatccgtgcc agcgattctt cacgcctcgc catgcgcgaa gccgaacgcc
     5461 acgttgaccg cgccctcacc attttgacca cctttgagaa ttgcgccgaa cgcgaagcgc
     5521 tcgaaaacct tgcaaaatat attgtggata gaaaagtgta agttagagaa cagagagcag
     5581 tgaatagaaa gtagaaagtg gtttccttca gttgcttctt ctttcttttt tcttttcatc
     5641 tttcatcctt cataattcat cctttccctc atgcccagtc ccaagaaacc cttccgcatt
     5701 aacgtcggct ttatcattta tgaagaagtc ggcaataact atgactttcc cttcgagttc
     5761 aacaagatcg tccttggtga tgacctcgaa ctgcgcaact ttgaaggcat tgtcaatgta
     5821 ggcaaaacgc cgcaggggct tgtggtacag gcggattttt ccgctgaaac cacgctgggg
     5881 tgtgtgcgct gtcttagcga tttcgatcat gaactcgatt ggtcattcac cgagttgtac
     5941 gccttcgaca aacgctccga aaccgaatct gcgctgatcc tgcccgagga tgcccatatt
     6001 gatctcggtg aattgttacg cgagtatgcc ctgctcgaag tgcccatcag cccaatttgc
     6061 aagcccgatt gcaaaggtct gtgtaccgaa tgcggccaga acctcaatga aaaagactgc
     6121 ggccatcatg aagatctgtc tgattcgccg tttgccaaat tgaaggattt gctcaagtga
     6181 ggcatgttgg aatgcggact tcagtccgca ttttgatacc agcagaatga agtctgcgct
     6241 gcgaagaaaa tgaactcatc cattcaaaaa acgcttggct ttatcgttac actggttttg
     6301 atcgggggga tgatctttct gcgcgcacgg ctcagttaca acacctttga ttttcaaagt
     6361 tccaatttca ccttcttttg gctttcaggg cggatgctga tcgaaggcga aaatccctac
     6421 gacgaaaaac aatatctggc ggggcatgaa acgtacggga tcaaatggca gcccaatcga
     6481 attttcccgt atcccctgcc gttggcgatc tttttcattc cactgggttt tctttccctg
     6541 cctgccgcct acatcacatg gcaggtcatt actttgttga tcgtcgcaac gacgatcttt
     6601 ttgctgcttg cgcattggca ggaatctgcc cagcgtctgc tgttggttcc tgtttttgca
     6661 gcaatgtttt tctttgggcc aatgtatctc accctgcatg cgggttcggt cggtgcgatt
     6721 gccttactgg cactcctggc ggcgattttg ctgctcgaaa aagataaatc cctcctggcg
     6781 ggaattgtcc tttcgttgac cgtgcttaaa cctccgcagg gtgttcccat tctttttttg
     6841 gcgggtatct ggtttttggc gcgacgcgac tggaaagcca tttacgggat ggtcatcggt
     6901 ggaattgcac tgttgttgat cgggatgatt caagacccga tgtgggtggt caaattccgc
     6961 ggtgcgagcg aagccgtaat ggatcggaca cagggagtcc actcgaatgt gtgggcgttt
     7021 gcataccttg cctgtggcgg gacttcaccg tgctggacgc ttcttggcgc cgccttgagc
     7081 ctgctcctgc ttggggcggc tggattcttc ctttggaaga atcacccaca gggggctgcg
     7141 ccagccaaat ggtcggcatg ggaggcgttc aatctcatca ttccgatcag tttcgtcagc
     7201 acgatctatc tttgggctta cgaccaaatt ccatacctca ttcccattgt gtggatcatc
     7261 ggtgtgttgg tagagaaatc gcgcaatttc atatatgcgt tcctgttttt gatcgtgctg
     7321 gtactttact cgctgtttgc gctggtgcag caggcaggca ccgacaagga tctgtggagc
     7381 ttgggcacaa cactcatcgt gcttgggtcg ctgtggttgg cctcacgcat gaaacaaaaa
     7441 ccacccattg acaaaccatc ttccacggcc taaaatacgc tcatgaaatt cagacccgaa
     7501 ttcccaatgg tgacaacgac catggccatg cgaatgcaaa tggagatgtt ttgccgcgag
     7561 tggaaagcac gttaggtctg tatacctgaa cgaaaacggg ctgtccaatt cgcggacggc
     7621 cttttttatt gcaaattagg aaaccatatg ggatgtttcc atctcacaat cccatccttg
     7681 ctgaccaatc atccaattcc ttgtggagtc tcttatgcct gaaaatatct tgatcgcagt
     7741 tgcctggccg tatgccaatg ctgaaatcca tgtagggaac ctgacagggt cacacctgcc
     7801 cggtgatatt gccgcacgtt accatcgttt gaagggtaac aaggtgttga tgatcacggg
     7861 caccgactcg catggcacgc ctgtcaccat gagcgccgat aaactcggca agcctgtgga
     7921 agaagtgtac aaatcctatc acgaaggatt cattgacctg ttccagaaga tcggcatttc
     7981 gtatgacctg tacacgacca cacacagtga gaatcatttc aaggtttcgc agaccatttt
     8041 tcttgcactg caaaagaatg gctacctgtt caaaaagact gagccgcaat ggttttctcc
     8101 tggattgaat cgcttcctgc ccgatcgtta cattgaaggc acctgttaca tctgcggtac
     8161 ggaaggcgca cgctctgacc aatgcgatac ctgcggaaat gtgcttgacc ccatgaaact
     8221 ggtcaacccg aaatcgaagg ttgagggtga caactcatcc cctgaactgc gcgatacaga
     8281 gcatttttac attgaccttt caaaacttga gcctgatgtt gtcaaatatc tcaaagaccg
     8341 tgccgatcat atgcgcgata ccgtgttggg cgagtcgctg cgtaaggtgg aggccgaagg
     8401 gcttaagccg cgcgccatca ctcgtgacct cgattggggc atccctgtcc cggtggatga
     8461 agcggggtgg gataccaaga aactctacgt gtggttcgag gctgtgatcg ggtatctttc
     8521 tgctcccatt gagtggagtc aggtatcggg taatggcgaa gcgtggcgcg attggtggac
     8581 gaatccaaac gcgaagcaat tccatttcat cggcaaggac aacatcttct tccacacatc
     8641 cctctggccc gctgaattga tgggcgttgg cagtgcgttc atggagttgt ttgcgggtga
     8701 ggaagttccg ctcattttgc cgtacgatgt gcctgccaat caattcatga atctcgaagg
     8761 caagaagatc agcggttcac gcagttgggc tgtgtgggga cgtgatgcgc tcacacgctt
     8821 cgaccctgac gccatccgct attacctgac catcaatatg cccgaaacga aggacagtga
     8881 ttgggattgg gctgagtttg tggcccgcaa taacaatgaa ctggttgcca catggggcaa
     8941 ccttgcaaat cgtgtgctgg ctttttgtta caagaattgg gaaggccatg tgcctgatgt
     9001 ggatttgaac acgcttcgcc ccgcggacct cgaactgctc gccgccatcg aaaacggatt
     9061 caacactgtg ggcacggaac tcgaagccgt acacctgcgc tcagccgttg gtgaggcgat
     9121 gaaacttgcc accgtggtca atcaatatct cgatgtcaac gcgccatggt ccgccatcaa
     9181 agtggacaag gtcgaagcag gaaagaccat ctacactgcg ctcaaagcga ttgactcgct
     9241 caaggtcatt tttgcgccgt ttctgccgtt caccagtgaa aagctgcacg gcttcttcaa
     9301 ttacgaaact ccgctttttg gcgagcaata cacccaggag gtaagcgact cattgggtac
     9361 acacaccgtt ttgcgctaca agcctgttga agggatgcaa tggaaaccca gcgatttgaa
     9421 gcccggtgca aagctcaatc aaccaggtcc gctgttcaag aagttggatg agatcgtatc
     9481 tgtggaagaa cgggcaaggc tgggaaagta aatgaaaatc ggatgtcgaa atgacatccg
     9541 atttttttat gtgtgcaaca cgcttttgtt actgctttgt cagcgtaatg ctggcaggtt
     9601 gtgaaggcgc tgcgcctccg cctgcggcaa tgaccaaatg cgagagcgtt ggaacggcaa
     9661 ggtcagcttc ggtgatggtg taggaggcgg tacaggtgag cgtgccgccg ggcaggatgg
     9721 tagctgcggc atctccgcag ttgatcgggg ttgcgccgtg caatccgccg ctgatggtga
     9781 attgagcggg gccgaggttt gtgctgccgc tgttcttaat ggcgtaggtg aaggtgataa
     9841 tttgaccagc ctgtgtgtac gtgagaggat ttgctgcgat cgtcaaggtc aggagtgcgg
     9901 tggatttgtt gacggtgaca ctggccgctt gtgagggtcc cgctccgccg cctgaagcgg
     9961 tgacttggtg cgagatcgaa ttaacggtca tgtcttcctg agtgacggtg taagtggagg
    10021 tacaagtcag tgttgcaccg gcggcgaggg ttgaagaagc agcgccgcaa ttcaatggag
    10081 tggagccaag caagcctccg ctgactgtaa attgggcggg acccaaactt tcagtaccgc
    10141 tgtttttgac agtgtaagta aaggtgatga cctgccctgt ctggttgtag gcggttgggt
    10201 tggcagcgac agttactgag agggttttgc tttgtgtcgc gggcggggtg gtgactgtgc
    10261 ccatggtgta atttggaatg cgcagtttga tgaccgttcc tgccgcactc aagctggtat
    10321 cggggttggc taattgaagc acagtggcat cggcgctgta tttttgtccg agggtggccc
    10381 aggtatctcc tgcctgaagg gtatgggata caaacgtgat acaaggcgga ccatagaatt
    10441 tggaataacc accaggatta ttcacggtca gaatcactcc gggcttgatc ttgctggggt
    10501 caggaatttt atctttgttg tcggctgcca gtttgttggg gtccacgccg taacagcggg
    10561 cgatctgcca caaccattca ccttcaacga cggtatgagt tctttgtccg ccgctgggct
    10621 gtggtgcggc atcgcttttg gtgacagtgg cccccacagc ctgcgaaact ggggcgccgc
    10681 cgcccgttgc ggttgcattg gttgaaatgg atgcgactcc catgtcagcc tgggtgatcg
    10741 tataagtggc ggtgcaggtc acagtcgcgt tggaagcgag ggttgcggtc gcttctccgc
    10801 agttgaaggg agcggcaaag gcggcgtctg ttactgtaaa ttgggcggga cccaaagtcg
    10861 ctgctccaac attggtgatg acataactat aagtaatggt ttgcccagcc tggctgtatg
    10921 tggtgggtgc ggcggttttg gtcagcttta aaaccacagg aggtacggtg gcaatgctgg
    10981 ttgtgacctg gttggaatta acattgttca ccgttgccat ggtagtgatc gtgacactgc
    11041 ccttatcgag gtcggcctgg gtgatggtgt aggaggaatt gcaggtgatc gtttcattcg
    11101 cgtcgaaatt ggcatcgagg ttgccgatgg tggtcatatc agggcagatg gcaccggtga
    11161 cgatcatcgg accgggcgca gaaaccgagc cgctgtttct gacattttgc gttaatttga
    11221 tgacctgtcc aaccgtattg taggtgaccg aggtgtcggc ctgtaccgtg agttcaagcg
    11281 ttaccacagc gggcaatgca gatgtggctt gcgccgccac ggcggcctgc gtgtcactgc
    11341 tgccaaatga gcaggatgcc agaagcaaca ggccaagtat ggaacttatg aacggacgaa
    11401 tcattttttt catttcgatt tcctctctcc aaaagaaggt tgcgggtaaa aactgtagag
    11461 gatatttctg cgtagtcgtt tgttgcaggg gagtatagca ttaatgatgg ggctgtgaga
    11521 acagccagcc gctcccccaa agttgccatg cgggtttgtg gtgattgatt tacattgaga
    11581 tgtaaccccg cccaaaactc gcaattgaca tacgtcttga tttgccctat aaacaataca
    11641 acaaaggagc agaattatgg acagagtacg ggcattggtg ctttctggcg gcggtggacg
    11701 tggtgcattt catgcagggg tttacaagta cttgatggaa tctggcaagc caaaagtgga
    11761 ttcagcgcat agcgcccaat gggatccgca ggtgatcgtg ggcacctcca tcggtgcggt
    11821 gaatggtgcg gccattgcac agggaatgcc tgccgatgaa ttggtgcgtg tttgggaatt
    11881 gctcgaagaa aaagatatcc agggcctgcc cccgggaatg cgcggtctgg ctcgttgggt
    11941 ggcacacaaa atcttcggaa cgatcatgga tgccaacctt ccacaagtct caccacagga
    12001 tgccacctca cccatgccga aagaattttg gccgcccctg ccgatcacat ccaaatggtg
    12061 gggcgagcgt ttggtgggtc gctggatcaa cttgctcgat acaggtccgc tgcgcaagac
    12121 cctgcacact catttcaaat tcgatccacg tgagatcgcc agcagccaaa agaccctgtt
    12181 gatcgccgcc accaatgtgc aaacggggga gcggatgatg ttcagcaatc gcgcgatcca
    12241 gcgacgcgac accggagagg cgcggcagga tgtagtgacc tcgatcactg ccgaccgaat
    12301 acttgcttcc tgctcgattc cactcgtcta cccgtggacc tttgaccccg aaaccaacgc
    12361 ccattattgg gatggcgcac tggtggcaaa cacccccatc ggcgttgcat tggatgccat
    12421 gcgcgatgtt ccgatcgatg tccctgccga gatcgtagtg gttttgatga caccgtggtg
    12481 gcagagcggt gaccccattc catacacggc acgcaagctc cctgaatctt ttggcgatgc
    12541 catcacctgg accctggatt ggatgctgct ggcttcattc cgcgaacgtt tgcggttgat
    12601 caaagccttc aatgatctgg cagtgcgtga acgtgctgaa ggcgctgggc cgtacaaata
    12661 tcgcgaagta aaagtgatca ttgccgcgcc cgatgatttt ctccctgccg agcgcattat
    12721 tgattatgac ggtgatgttt ccagagacct gatccagcag ggatatgctg ctgcgaaaaa
    12781 ggcctttgaa gcagcgttct aaaaggttcc gtaaatgcct ggttgaaagg cggcactcac
    12841 tgtggatgca agtgctgcca gaaacaggaa aaagagaccc agatcatacg ccaattgaaa
    12901 aggggttctt gttgcaggtc ttgtgctggt gaggatgtca actttggctt gcagccagcc
    12961 tccaactggg agcgaaaata taatgcctgc agcgagcgcc agaaggaagg tcggttcaat
    13021 gaatggcagg gggctggtcg tttcaaaggg gagggcttgt attccactca tattgcctgc
    13081 aagacggcgc agaaaatcaa aggcgaagtc aggcgaaggg gaacggaaga atacccagcc
    13141 tactaagatc acacccaacg catacacatg ttgaacaggc gcccaggcag tacgcagttt
    13201 ttttccaagg gtggtggctt cgaagatcaa tgccatgccg tgaatcagac cccagatgag
    13261 gaagttgcga gttaatccgt gccagaggcc ggtcagcgca aagacgatga gaatgttgag
    13321 cggctggccg agccatttca ggcggcggcg ttcgagcgga taaaaaacaa aatcacggaa
    13381 ccagtttgag agtgaaatgt gccagcggcg ccagaaatcg ccgatgcttt ttgaaatgta
    13441 ggggaaattg aagttttcca gaaagcgaaa gcccatcatg cgcccgaggc cgatggccat
    13501 atcggtgtat cctgaaaaat cgaaatatag ctgcagggaa tagctgataa tgaccagcca
    13561 tgcgaacgat ggtgcgatgg tcggggatgc cagcttgaag atcggggtga ccacccttgc
    13621 gagggtatcg gcgatcaggg cttttttggc gaatccgcga atgtagcggc ggagaccttc
    13681 tgccacctcg atcgggtcaa cctgaatgtt ttgtatctgt tcacggagtt gacggtaacg
    13741 ggtgatcggg ccaacaggaa ttttggggaa gagcaaaagg taaaaggaaa aagcaagcag
    13801 gtctttttca cttgtggatg ttttgttgta gacctcaagc aggtaagcga tcacttgaaa
    13861 tgtgacgtag gaaagcccca atgggtattt gatgcccgtc cataatttga aaatgaccag
    13921 caatgcgatg ttgatcagaa tcccaaacca tagcaggatt tgcgctgttt tttgtccacg
    13981 ccaatgctcg atgccgcgtg ccagaaaata ggtgaatatg gtcagtgtca ccatcaatgg
    14041 gacgtagatg aggttgcccc atgtatagaa taacaaactg cctgcaatgc cgatgataag
    14101 ttttccacgc tggggggcga agaagtaacc cagcatgaac cagggcagaa atagaaaaag
    14161 aaagttgggg gaattaatgt ccataaaagt tggcctgccc tgtggagggg acaatgtgtc
    14221 ggcgcgatta taacaataaa ccgcctccca gttttcgggt aagtgggagg cggttttaca
    14281 tccacaaaaa aacagggatg acccaaatta tggtcatccc tgagaaaaga tttgctcttc
    14341 ggcagcctgg cgatcatgat cacaaaaata aagttgtgat tttagacccg tggctttgcg
    14401 tcatcaggtt ttcctgattt tgcgtttcgg agggtcggtc ggtcttgatc aactgtacaa
    14461 cgtgcgtact tgctgatttt agacgctctg actctgcgtc gccgtctttt ggcggttttg
    14521 cttttatcgg tagtctggct gccatgactc agttcagctt taggcccatg actttgcgtg
    14581 accggggttt ccccggtttt gccttttttc ggagcaatta aattattgca cgctcgaaaa
    14641 atattttcaa tcactcagat gttctattta tttaaacgac attccgccgc cagaaatggc
    14701 ggcggaatga ttttgaatct ggctttgtaa atatttattc ctgaacgatt tccatgaagg
    14761 tgacttccac cgtcatgccc cattttaggc gcggatcggt ttcctttacc gcaatgcgga
    14821 cggtgtagag aatgtcgccg ccttgctggg tgaaggcttc gctgatctca gtgaccgtgc
    14881 ctgtgaggac gacatcggac agggcatcgg gtacgaaagt gacgggttgg ccttcgctca
    14941 gttttacaac ttcgagttcg gtgatgtcgg tggtttcgat gacccaagaa tcgaagtttg
    15001 cgatgctcaa cacacgcgtg tccacggtga cttgttcgcc tgcctttatg ttgacatcgg
    15061 ccacgatgcc atcgaagggt gcagcgatta cgtaattgga aagggtgtct ttggcagaat
    15121 cgagattggc tttggcaaga gtgagttggt cggcgttggg gccatcaagg ctgatctcgt
    15181 attgatattt tgcttcggct tcaacagcga gagcggcatc gtatttggcg cggacttcgt
    15241 cgcgttcgcg gatttgttct tcgaggttgc gaatggcctg gttgagatct tcctgcgccc
    15301 gttcgagatc gtcttcagcg gctttgcggt tggaattatc ttcgtcgagg tctttgtatt
    15361 tttcgaattc atccaatgcg tcttgcaggt cttccttgcg gtcttccaca gtggctttgt
    15421 catcttcaat attgtcttcg atgttatcca cgttcaggtc gtcccatttc tgttcagctt
    15481 tgccgcgttc ttcctgcgcg accatgtaag cctgccagag tttggcccgg tcaccgcttt
    15541 cgttgcggag cagggtgtcg taggcatttt gtgcaatgac gacttgcgct tcagccacgc
    15601 ccgcatttgt cagccgcgcg aggacatcac cttgtttgac agagtctccg gctttgacga
    15661 ggatctcttc cactacgccg cgtgcttgaa aggaaaggtt ggttccctga atgggttcaa
    15721 ggcggccttc tgccaccact tcattggagg taactacatc ttctgcaggg gcaggggtgg
    15781 ttgcgggttg ggttccacac gccgcaaggg cgagggaaaa aatgacaagt attgctgcaa
    15841 tgtttagagt ttgttttttc attatttatt taccttctta ttatggatgc cgttggtgcg
    15901 gcactttttt tattcgtatg ccagcacttc tcgaatggtc aggcgggcgg cgttgcgggc
    15961 gggcaaaatg gagccgagca tcgaaaggaa taacaccagc ccaaaccaga tggcatagcc
    16021 cagataagtg aagaccacat caatgggggt ttcaaacacc gccagactga cgatatagga
    16081 aagcccgtag gtgaatggga tggaaacccc aattgccaga acaaatgaga tcgtaccgat
    16141 cacaaaacct tccgcaataa tggtgcgcat gacggcacgg tcatctgcgc cgatggcgcg
    16201 catgatgcca atttcgcggg tgcgctcaag gacattcatg cccatggtcc ctgtcaaccc
    16261 catggcgccg acaatggcgg taagtacggc catgatgagc aaaaatgcaa caagaatgtc
    16321 gaggctttca agcgcgctat ccagtgatgc aagccctgat tcagccttgc ggactttgaa
    16381 gccttcatca cggaagaaac ggtccagttg ttcggccatt tgatcctggg aaatgcggtc
    16441 atgctgcaag gtgacaacgc ggaaggaaaa agactggttg gggaggttgg tgatctggac
    16501 ggcatattca taagggatgt aggcaaatac accttcctga ccgacaaact tgaacagccc
    16561 gatcacttcc caggtttcct catggccgtt gaccttcaag aaaatagggt caccgggttg
    16621 aaggcctggg taggtatcga gcactttctc gctgatcaac aatttgcgaa catctgtggc
    16681 ttcgagccat cgccctgaaa ccagaattgg ctcaaccaat gtactgtctg atgggggcgc
    16741 aaagacattc acgttttccg ccactgctcc atcggggtgc aggagttcaa acgacaggaa
    16801 ttgccaacct tccactgagc ggacatttgg gatttgcagg gcgaattgtt ccacttcccg
    16861 caggcgatat ggcttgtcga aatcgagtgt gacatcggca cggaaatact ttccgatgtc
    16921 gttcatgtaa ttgtgcaaag tgacgcgcac gttgaacacg gcgataaaaa ttgcgccacc
    16981 catggtcaga gtgaagaggg tcaatatcaa acgtccgcgg cggcggaatg tgttgcggat
    17041 ggagatgata aaggggcgag ggaagtgaat gccgcgtctg gcgaacatgt ttgtgaggcg
    17101 taccagcatc cagtcgaacc aattgaggcg gtcagtctgt ttgtgaggtt tgttctcgtc
    17161 gcctgcgaca tctccgctca aggcgcgcag gaccgtgatg cgcgatccgc ccacaacggg
    17221 cacaatccct gcgatcaatg gcacgagcaa gccaatgacg acctggatca caagtgccat
    17281 tggcacaatg cgataaccaa gcaggtcaaa ccttaacttt tcggccatga attcggccag
    17341 acggtatgca ccccatccac ctgaaggaac ggcaaccagt aaggcgagaa aactgaatgc
    17401 catgatgagc gcgatataca tggcaaagac ctgcctgccc cggccgccca ccagtttgat
    17461 cacaccgata tggcgcaagt gttgacttag cagtgcatcg agtgtgttgg cgatcagaga
    17521 acttgaaagg aaaacgatca ggattccgag tgccagcaaa atactgagaa tggcagtgat
    17581 gatatctgca agcgggtact tgtgcttttc agaaaagcgg gtgcgaatga catctgtgcc
    17641 atttctttcc agtttgtctt tgatatctgc gcctgcttca tggatgtaat ccgaatcgtc
    17701 cacgccgttg gaaagggtga tgtatgcgcg gttgaacagg tcatcgggtt ggcgcagggt
    17761 gggcatggtt ttcatggaaa tgaaaccaag cgaaggcgcc agaaagtccc cggcactgga
    17821 gcttgaatcc tggacgatgc cagcgattct caattgcttt gaagtgccat cgggcaattc
    17881 taggataagg tagtcgccga tctcaaaatc gaatttttca agggcccgtt tatcgagcag
    17941 gatctcatcc tttttcgctt cagacgcccc tgcaactggt ttcaataaat tgactttatt
    18001 gtccttgaaa cttttaaacg caatcacgtc cagtgatacc caggcgttgc tgtcaatggt
    18061 gcgtgcccgg aagttgatga cccgccttgc ttcggcatct tcaatcccgc gcatattgcg
    18121 gatattgtcc aatacatcct ggtcgaaatt cgcagtccgc agttcaatgt tggacggatt
    18181 gttggccgcg taggaaatac ccatgtcatt gctgatgatc tggtacgcac ctgcgatcac
    18241 cccaatggaa aatacgccca ctgcgatgga gaataccacc agcagtgtgc gggctttgtt
    18301 atcccataaa tcatgtaaaa ctttacgcca tcttggtcgc atattactca cagagacctg
    18361 acaggtcgtg aaaggcctgt caggtcttga tataaattac ttcaataatt cgcttacttc
    18421 cccggcagag tagggtttta cgttcacatc tttcatgcca ttgtgcatga attgtgttac
    18481 ccgttcgggg ttgatcgccg aggtgaggac tacggtcttt gcagcaagcc cggctttgac
    18541 gatggcatcc aatacctcgg ccagcggaag ggatgtgatg ttttcatcca ccagaatcag
    18601 gtcggcagcg ggcaatttct tcaagtcagt ggtctgggtg agtttgacat ttgagccttt
    18661 gagcatgttt gaaaaatgat tggcccaatc atcgttgtcg tcgatcaaca acaggttttt
    18721 cacactgccg gtcttgagcg gttcatctgc cagaatgcct tttggcaggt acatggcgac
    18781 cgttgaaccc ttgccctgtt cactaacgat ggcaatgcgg ccttgcgcct gtgaaatgac
    18841 atgcatggcc gctggcaggc caagaccatg gtggtctacg ccgcgagtgg tgaagaatgg
    18901 agaccaggcc ttttccatga catcacggtc catgcctgaa ccgttgtcgt caaccatgat
    18961 ctcgaggaaa ccgcgttcct cggtttcgtg tgcggtgatg cggatggatt tggcgccagc
    19021 ttcggcggca ttttgcaaaa tattacccac agcgcggaca agttgggtgc tatcggcaat
    19081 gacatatgcg gcggcagggt caattttcac tttgagcatg tcgctggaaa tatcacgttg
    19141 gtgggccgcg gtttgaacta catcagcaaa cagaacaggg cgcggcttct gttcgcgcac
    19201 ggcaccgatt aattgttcct tgacctgaat gatctgtgcg gcgctctcag cgatcatctc
    19261 aaggtcttct cgcagggagt ccacatccac ctggccttcg gcaatttcct gctgcaggcg
    19321 ggaaacggtc aaagagatcg gcagggtctt gtttccgatc caatgaatgg cttcagtgga
    19381 ggcggtggtg agtgcttcaa agactttgtt gcgaaggatt tcatcatgtg cttctttgag
    19441 ttcgtcaatc aggcgggcgt tgttgatcgc caccgccaga tgttcggcca tggtgatcaa
    19501 tgtggtgata tcgtcttcac tgaaggcacg ttcctcggag ctttgaatgg tcaccgcgcc
    19561 aaggactttg ccgccgtaaa tcaggggcag agccatttct gagcgggtgt gcggcaggtg
    19621 cggatttttg aaatgtaccc gttcctcacc cacatccaat gcgatacggg cttcgctcat
    19681 ggcgatacag gcaccgatca tcgagtcggt tccgatcttg agtttgtgac catctgccac
    19741 catggctttg cctgccttgc cgtagccagc gcgcagaaca gcatactcac cggcctcatc
    19801 cacaaggaag acgcccgcgt agtacaagcc gtaggattcg caaatgatat tgacggtttg
    19861 cggaagaagt ttttccagat cgagaatgga tgtgacttcc ttgccgacgc ggttggcggc
    19921 ttttagcaaa cgggaacggc gttcggcctg acggtgcagg ttggagtttt gaatggcaat
    19981 cgccaactgg tcggccatgg tttgcagggc ggcgatatct tcatcatgga aggcagcttc
    20041 ttcggtggat tgaaccgtca acgcgccgat gacatcttcg cccacgacca atggaagtgc
    20101 catttctgaa cgtgttttcg gaaggtgggg gttctcaaag aacaccgctt ccgcgcccac
    20161 atccagcgcg atgcgtcctt ggcggtttgc gatacaggct ccgatcatgg agttcccgcc
    20221 gactgcgagc cgatgcccgg cgtggatcat ttccctgcct gcttcgccgc gccctgcttt
    20281 gaggacagca tattgcttgg catcatccag gaagaatacg cctgcgtaat aaaacccaaa
    20341 ctcatcgcag atgatatcta cggtgcgctg gagcaggtcg taggggtcga ggatggtggt
    20401 gatgttcttt gcggcgcgcg cagcagcttt caacaaggtt gcatgtcggt gaatatcgtt
    20461 tgtcataatt tcctcttttg gattaagtgt ttgaaggttt acaagttgaa agttaaagga
    20521 ctagcctgtg gcttgtaatg tcttttggat caaatccacc agaccctgtt cgcgaccttt
    20581 gacgaagaac gcgcttgctc cgccggaaac ggcatccttg gccttggtca cttcatcgta
    20641 cgcagtcaga atgatgacgg gcagagatgc cttttgtttt ttgagatcag ctaacaggtc
    20701 cagcccggca ccggttttgg gcataccatc gaacccgggc atgtttaaat ccagcacggc
    20761 gatattgatc tcgcccatgt tttgggcaaa ggtttcgctt ccgctcttgc aatcagaggc
    20821 tgtgatcaca tcgaaacctg cgcgcaaaag ggtcttttcc aggcttcgta gaatcaactt
    20881 ctcatcgtct actaaaagta cagttgtcat tcgtcctcca aaataattta ttcacgaagt
    20941 gcggacttta gcccgcgacc atcagtacgc agactgaagt ctgcactccg ttaggttttc
    21001 ttttcaacgg gcaggcgcac ggaaaaggtt gcgcctttgc ctgcttcgct ttcaagtgaa
    21061 atgctgccgc cgtgctggtt cacgatctgc atgactgctg aaaggcccag ccctgtaccg
    21121 ccgctgccgc ctttggtggt gaagaatgaa acccagatct tttcctgaat ttcctttgga
    21181 atgccagggc cattatcttt tacagtgacc agaacaaacc cagggtcaga atcgcgctga
    21241 ccttttacat ggatatgcgg ctcgggcgtt tgagacatgg cctcccaggc gttcttgatg
    21301 aggttgttga aaacctgctc cacctgccgc gcatctacaa aggcattcgg cacgttgtcg
    21361 gcccaatcca tttccactac gccatcgggc aggcccatgt ttgctaccgt atcagcgatc
    21421 atgtcgcgca gtgatacggg cgcgtcgttc ctttgacggg cggggccgat caaaccttcc
    21481 ttgatgctga ggatggtctc cgcgctgttt tcggcgatgt ccaaatcttc catcagcgat
    21541 tctacgctga ccaatgcccg cagacgattc ggtttcatgg cagacatttc agccagaatc
    21601 tggatcaggt caatgcccat tgcctgcgct tcttccctga tcgtttcaac ggcttccatc
    21661 aatgacacgt tctttgcatc cagatcacgc ggggcatatt ctgccgccaa tgcaaggatg
    21721 tagcggatat cttcacgcgc acgtttgatg ctgcccggga tcggggcggc tttgttgccc
    21781 acccaatgaa tggcttcacc tgtggcggtg gcaatggctt cgaaagtctt cgagcgcacc
    21841 aattcagcgg tggcatcttc cagcttgaac attaactggg cattgttgat ggcgatggcg
    21901 atctggtcgg ccatggtttg cagggcggcg atatcttctt ctgtgaatgc attcaattcg
    21961 tcgctttgta cggtcaaacc gccaagggag cgtgatttga cgatcagcgg taatgccatt
    22021 tcagaacggg tatttggcaa aaatggattc ttgaaccgcg attcttcgcc tgccacatcc
    22081 aacgcgatct gtgcctgctg ttggatgatg gattttccga tcatggattt ttcgtcgacg
    22141 ggcaggcggt aattttctgc catcatcttg cggccagctg cggcgtagcc tgcccgcaac
    22201 accgcccagt gaccatcttc ggaaaccaga aaaatgccag agtaatagaa gctgaactca
    22261 ctgcaaatga tgttgaccgt atgtttcaac aggtcatcca aatccaaaat ggaggtgacc
    22321 atttgtccaa cacgcgccgc ggtttcgagc aatttgtgcc tgcgctccaa catggagaac
    22381 atgcgctcgt tttgctggag caggtgtttg ttcttttcga ccgtgttggt caactgacgg
    22441 atgttgcctt cgagccgttc caccagttcc tgctgatacg cctgcaagtg ctcttcagca
    22501 ttatcaagtt tttccacccg tccattgagg taggcatcca cttgatccac aaaatcggcg
    22561 tcaatgggtt tggagatgaa acccaccgcg cccgctgcaa gagcccgttc acgtacgcca
    22621 gggttcaggt cacctgaaac cacaacgatg ggtgtgttgg gcagcatttt gctcaaacgg
    22681 gtggccgctt caatgccaga catatgcggc aggctgttat cgagcagaat caggctgggg
    22741 tgtgtttctt cagcaagctg aaaaccatcc agtgggtcag aggcctcaag aacaacatat
    22801 tttttggaaa gcaaacgcct taccagcgag cgggtctcgt cgctgtcttc tatataaagg
    22861 atttgcgggg tacgacgtgg gtctggcatg ttcataaaaa agcctcaaaa cctgaaatgc
    22921 tttatggttt tgctgcgagc ttatcttctt tcagggcttc cattttctcc tgtgccactt
    22981 tcaacaggac ttcggctgtg atgggggatt cttggatcac ccgtgtgaag tcggcgcgtg
    23041 gaatggtgag cagctccaca ggctcgcttc ctgcgcggac ctcggtcagg gcgccgccgc
    23101 cctgcaaaag ttcgatctcg ccgaaaaatt gacgagggcg cagccgcagg gcggtttgtc
    23161 catgcagatc cacttcacct ttgcggatca tgaaaaaatg ctccacagcc tgaccgcgtt
    23221 gaaggatggt ttcgtttggc gataccatgc gttcttcggc aaggcgggtg aattcaagca
    23281 tgtggcggtg acgcagttgc ggcagtgcgc gtgaaatggt ttcgttgatc agttcaccat
    23341 ctgagatgat gatgttgcgg ttggtgcgtg aagtgagcga cgggtcatgg gtgaccatga
    23401 caatggtttt gccctgtgac acgaacaact cgaacagtcg gatgatggta tcggcggaac
    23461 gtgagtcaag gttgcctgtg ggttcatcgg ccaccagcag gggagggtca caggccatgg
    23521 cgcgggcgat ggcggccagt tgctgctggc ccgtggaaac cagcacaggc agtttgttcg
    23581 caaatttttc aaggcccacc agttttagca attccatggc acgggtgggg cgttcgtcaa
    23641 aatcgtacat gtccacataa tccattggga gcatgacatt ttcaagcagg gtgagcatgg
    23701 gcagtaactg aaagaattgg aagacgatgc ccaggttttt tccgcgccat ttcgagcgct
    23761 ggctttcaga gacgcctgtg taaatatcgg tgccattcac caccaccaga ccatcggtgg
    23821 ggtggtctat gcctgtgatc atgttgagca gtgttgattt gccgctgccc gattttccaa
    23881 cgatggaaac gaattcaccg cgattgatgg tgaggtctac acctttgaga acggtgaatt
    23941 ctcctgcagc gttgctaaac tttttgacga cactgcgtag ttcgatcatg gattcaggcg
    24001 caaagtctgc tacttgcgcg ggaccagatt tcggctgggt gcgaagcggg gttttgttca
    24061 tctccgcctc ctgaaggttt tatccagttg tggttcgggg tcttctgtgg cgatctctcc
    24121 atcggtgatg gtgaggctgc gtgaaaaacg gctggccaga cccatatcgt gtgtgaccat
    24181 gatgatggtt ttgtcttgtt cgctgaccag atgctcaaag acatcgaaga tgtgatcggc
    24241 agtgatggaa tcgaggctgc ctgtgggttc atcggcaacg aggatgggcg ggtcattggc
    24301 gagtgcacgc gcaatggcga cgcgctgttg ctgcccgcct gagataaagg ctggcagttt
    24361 gtaggcatgt tcttccagtt cgacgaggcg cagtaattcc atggcgcgtt cctttgaacg
    24421 acgcgggtgg aagttgttgg agagatccat ggggagggtg atgttttcga tgagtgtgag
    24481 catgggcaga agttgaaagg attgataaac cacgcccatg ttttttccgc gccagagtgc
    24541 cagtgcatct tcgttcatgt catgaatgga gacgttcttt ccgttggaac tgacgatcac
    24601 ttcgccgctc gtaagctggt ccacaccatt gatcatgttc agcagggtgg attttcctgc
    24661 acccgattta ccgacaatgc ccagaaattc gcccatttcc accttaaggt tgacttcttt
    24721 gagcgcaaca aagtcacctg cggcggtgga gtaggttttt gatacattat ttaattcaat
    24781 taaagggttt ggcataattt tcggcttgat tttaccgcta ttgtgcaatt ggtagcaaat
    24841 aacgggtttg caaagggttt tcaattgatt gttttggtaa ttttcctatt ttcgaaaccc
    24901 caaacctttg gcgataaggt tatcaaaaac ccgtttgggc agcagcatgg ggagaatcca
    24961 gtttttgagc atgttcggta ccacagggta gcggactttt ggtttcggtg tggtgagggc
    25021 atgtaaaacg acttcgccga tcttttctga tggcaggcct tttctgccgt cttcgaccat
    25081 gtatctttgg aattttttgg cagcctcgat gtattctgtg tgttcgtatt ttgtgaggtc
    25141 ggcctgttcg cctttatccc agatcggggt ggctacggcg cctggcccaa tgatgatgac
    25201 gtcaattccg tacagcatta attcgatgcg cagtgtttca gaaagccctt ccagcgcatg
    25261 tttggaagtg acgtaggcgc ccacaaaagg cgcgcctcgt ttgccgctgg tggaactcat
    25321 gttgatgagc ctgcctggct ggcctttcaa tgaatggtcc atgccgagca gggggaggaa
    25381 ggcttgcgtg acgatgagct gccctgtgag gttgatttcg atttgactcc gaaactcggc
    25441 gatcggcatg tgcgccatgg gagcggggat ggcaatgccg gcattgttca ccaggccaaa
    25501 aagggtttcc ccgttcagtg cttcacgtac ctgcctggct gcggcatgga ctttctcttc
    25561 atcggttaca tcgaagatca atggggaaaa tgcatctccg aattcggctt taagccgttc
    25621 agcatcctgt gttttgcgca cacttccgaa cacgcggaat ccgttttgga tgaggacttt
    25681 tgtcactccc cagccgattc ctgttgaaac acctgttacg acaacgcttt tcatttgcgt
    25741 ctccttttca ttttttaact cgttcgataa acggaaagag aaaatttcaa ttgagtcaaa
    25801 tgattataat cttgcctgtc accctgccgt tgatttgaag tttggatgta atagactttt
    25861 tgtaaaaaat aaccactgag ccacggaggc acagagagaa cgattgaaag ctcagtgcct
    25921 ttgtggttca acttcaaaga aatttctggc gctcaaccac caggctgatc gtttggttta
    25981 aaagcagggc aagggaggta aaatagaaaa aaggttaaac aacagagaac gaattaaccg
    26041 atcaacaacc catgctcagg aattttcttg ctgacttaaa ggatgtatcg cctgcctgca
    26101 gagaatattt tctcaaggca gattccctgt tgtctgcatt ggcaattggt atatctgcgg
    26161 cggtcattcc ccccactgcg gttttggatt acctgtatta tgggtttaca gaaaaattct
    26221 ttgcaacagt tttatttgag ggggtgtttg ttgccttgtg cctgcttgcc atggtggctg
    26281 tcaggcacgc ctacactgtc gccgtttacg attgggccgt atttatctgg acattcatgg
    26341 cggcgatcgg tttttccatc atggggttcg atcagcctga acgcgttacg gaaaatattc
    26401 tggttgttgt catttttaca acggtgatct acacccttgt tacaaaccgc ctgttatttc
    26461 gtctcatccc tgcctggatg atgatgatct cgtatctggt catcttcggt tcagatctgc
    26521 caacggcgat cattccgcag caggagatct ttttcagtgc atctttgctt gtggtgaata
    26581 tcggcgggct gctcattgtc ggcagttcca accgtcacaa gcaggatgat taccaggcgc
    26641 gccaaagcga aaaagaggcg cgtcagcaat tggaaaccct cgccagtatc gacccgttga
    26701 cgaatatttt caaccgtcgg catttcatgg aaattttcaa ccatgagttg gaccgcggta
    26761 aacgctatgg ggcgcgtttc tccctcgttt taatggacct tgaccatttc aaacaagtca
    26821 acgataccta tggtcatctg gcaggggatg aagctttaaa ggcattcgtg cgcatcattc
    26881 aggagaacaa acgcaagacc gatatctttg ggcggttggg aggtgaggag ttcggcttgt
    26941 tgatgatgga aacctcagga gcggatgcgc tgactacact tcaacgattt ctctccaaag
    27001 caaggcagga acatatcgtt atggatgatt cgaccttcca tttcaccttc agcgctggga
    27061 tcaccgagac actgcccaaa gaccgctcca ttgatgagat cattcaccgt gccgatgaag
    27121 ccttgtataa ggcgaaagaa agcggtcgta accactgtgt tctggtgtag ccgattatcg
    27181 tttgcggcca aataacacac ctaaaatgcc aatgaccaat accactgggg agcacagcat
    27241 gaatgtgata cccataaata gaaaactgga agcaagatcc cgttgatatt tttcgaaggc
    27301 aaccctgtca cctgcaaaaa gctgactggg aaccaccccg ccgacggagg cctttttgcc
    27361 gaacacggtg gtgagatcgc cgttggcaag acctttatag cgcatggcgc catcgtgcag
    27421 ggactggcct tcatatactg cctgcaggga gcctgtccca tctacgagtt tctcgtgtaa
    27481 agccccgtta agtgtggccg tattggatgc ctgaatggaa acagatgtgc catccatatc
    27541 cagggttagc agaggcacaa ccgtttgcac acgggtccag gatccggatg gggtgttgtc
    27601 gtttccgttt tcatttttgg aaacggtcac atcccagcgt tcttctgtat aggcaatgaa
    27661 ttcaggcagg tgaggggttg gcgcatcccc gttgataatg ccggtgatca acacatcacc
    27721 gcctggggca atggcttcca cagattgggc ggtcatcagc ggtatttgcg agaccttgta
    27781 ggccaggatt gcttgattgg gcgccaacac gaaggtcata aatgcgccgc aggcaaaaac
    27841 agaaatggat gcagtaaaga ctccgaatac tgccttgatg cgatttctta attgagcgaa
    27901 cagattgact ccaaaaaagt tattcattgg atcggctccc aaagttgata tttttgcgga
    27961 taaatttttg caattctatc gcttgtttcg taataaagta aatttttttg aaaactgcaa
    28021 gtgagaaaaa actgttgtat accaaattat ggaatgccct gtgacctggc aaagatatcc
    28081 ttccaaccga caaagttggg aaaatgaaag ccccatgcca atttgagtca aatggtttac
    28141 aattggcaag acctatggag aaaaccatga acggtgaacc tgtgttggta atgttggtgg
    28201 aagataacat tgaccatgcc gaactggtga tcaggacttt ggaagatcac cggatcgcca
    28261 acaagatcaa acactttatg gatgggcaga ctgcgctgga ttatcttttc aggcgcggtg
    28321 agtatgaaga ccccaaatcc agccctcgcc ctcacatgat actacttgac ctccgcctgc
    28381 cacgtgtgga tggtcttgag gttctccatc aaatcaagga acgagatgat ctgaagaacg
    28441 ttcctgtgat tatcctgacc acatcggaag ccgagaaaga tgtcgcccgc gcctacgata
    28501 accatgtcaa cagctatctg gttaaaccag tcggattcga tgaatttagc aagcttatga
    28561 atgacctcgg attttattgg ttgggttgga atacgcaccc tcgcatttaa ctgtaatcaa
    28621 tttgaaagca caatatatgg atgacaggat caacatcctt ttaatcgaag acgaagaccc
    28681 tcacgcagaa cttatccaac gcgccttcga agaccaggat gcccggtttc aaattttccg
    28741 cgtgcgctcg ttgacagaag cccgagccca actggccgaa aaagaccccg cccttattat
    28801 tgcagattgg cgcctgcccg atggggaaag catggaattg cttcccgatc atcatgaccc
    28861 gctttccact cccattattt taatgacaag ttatggcaat gagcgcatcg cggtcgaggc
    28921 gctcaaatct ggtgcgcttg actatgtggt caaatcaccc gagtcaatgc ttgatatgcc
    28981 ccatctcgca gaacgagcca ttgagcagtg gggagcccgc gccgaacgtg ccaagatgca
    29041 gaaggctttg ctcgaaagcg aagcgcaatt ccgcctgctt gccgagaacg ccagtgacat
    29101 gatctcgcgc ctcacgacgg atggacgtgt gctttacgtt tcgcccgcct gcgaaaccat
    29161 tctcggctat accccggaag agctgactgg caatagcgcc tttgatatcg tccatgaaga
    29221 tgaccgtacc ttcgtcagga acctattcaa aggcaatcgt tatgatgcca cctacaccat
    29281 tgcctatcag gctctgcata aactcggaca ttacgtctgg ttggaatcat cggcaaaagc
    29341 cattctggac cgcaagacca ataccatcag cgaaattcag atcgcatccc gcgatatcac
    29401 cgagcgcaag aaatctgaaa aagccctgca agccgcacat gaaggtttgc aggaagccta
    29461 tcaacgcacc attgaaggct gggtgagagc attggatctg cgtgaccgtg aaacagaagg
    29521 tcatacgcaa cgagtcacag aactcaccgt gaaggtggcc agaaggcttg gtttctctga
    29581 agaagagctg acgcacatcc gccgtggggc attgctgcac gacatgggca aaatggccat
    29641 ccccgatgaa attttacaaa aaccaggccc actcaacgaa acggaatggg agcggatgcg
    29701 ccgtcatccg ctgtatgcat atgaaatgct ttccccgatt tcctatctcc atcctgcact
    29761 tgaaataccg ctatgtcacc acgaacgttg ggatggcagc ggctatccgc gtggattgaa
    29821 aggggaggaa attccacttg ttgcgcgttt ctttgccatt gtggatgttt gggatgccct
    29881 ttgcagtgac cgcccctacc gcaaaaaaat gccccaacag gaagtgatga actacctgcg
    29941 tgagaaatca ggaatcctgt tcgaccccaa attggttgat gttttcctct ctgtggtgga
    30001 gagcgaagag aaataatatc ttactcattg aatggctcat gttgacacgc cccagtacat
    30061 cgggtatcct tgcccccgtc cgggcaggcc cggcgcggcg aagcctttcg aaccccgcca
    30121 ggatcgaaag atagcaacgg taaggaagag tcttcgggtg ccgccggtag cctgcccgga
    30181 tattttgtcc ccatcatttc actcccactt atgaaatcat cgaactggat catgatcatt
    30241 gccgccgcca tcattggcat tggcatcggg ttgacctatg gctgggtcat taacccggtg
    30301 gtgtataccg atgtcacccc cgatgcgttg cgcgccgatt atcgcgccga ttatgtgctc
    30361 atggttgctg aagcctatca gagcgatcaa aaccccgaaa cagccgcccg ccgcatcgcc
    30421 atgatcggaa gtgcctcccc agaccagatc gttgaagagt ctttggatta cgccaccgca
    30481 aacaacttta gccagaacga gattctgctt ttgcaaggct tgctcaccgc catgcagacg
    30541 tatcaaccac aaccggtcaa cgccccatga gatcatctaa ccgtaacagt atcctgtaca
    30601 tcgtcctcgc gcttcttacg ggacttggac ttggtctggc gtactcgtgg cttctctccc
    30661 ccgtaaccta tgtggatgca acgcccgcca tgctgcgtgc cgatttcaaa gatcaatatc
    30721 gcatcgtggt ggccgcctcc tatgcttcat cgcatgacct tgcccgtgcg cgtgcccgcc
    30781 tgcaattgct tggtgatgtt gaccccgttg ggcagctcag tgccttggcg caaaatatgg
    30841 tggctggcaa tgcttctctc gaacaagtgc agcccattgc ccaactcgcc actgacttgc
    30901 aacagggatt ttcaagttca ccagtgacag atacaccttt cgtggttgtg gtcaacaccc
    30961 ccactgaaat ggatacccct tcagcagaat cgctggccac tgaaacccag atcgttcttg
    31021 aagaggatac acccatcccc acccttgaat ttgaccagac cccgctcgcg ccaatcgtta
    31081 cctccacttt cgtgccgcgc cccacgttca cccctgttcc agtcagcggc gatgtcttca
    31141 atcttgtcgg tcaagatacg gtctgcgacc ccgggcttca gccaggcttg ctgcaattca
    31201 tcatcatgga ttcgcgccgc agacaggtgg caggcatcga gatcatcgtt tcatggagtg
    31261 aaaaagaaga ccgtttcttc actggcttca aacccgagct tggagatgga tacgcagatt
    31321 ttgtcatgca gcctgatgtt atatacagtg tccatgttgt tgaagggggg gccatcgtcc
    31381 cgaatattgc cgctcccacc tgcaccgacc cgaatggaat aagttatttc ggcggattgc
    31441 tgcttacttt tcaacagcct taatgcaggc tggctttgca accaatccct gcttcattcg
    31501 ttatatctta cataaaatga tcaaggagta cgaatgaaca catcttataa caatcaaaaa
    31561 ccaggtttgc tgacggccat catctggatg acccttgcca gcggaattac caatctcttt
    31621 tggggatttg tggcatcagc cactgcgctt ggaacaatta ttggggttat ctgtttgcca
    31681 attacgatcc tgccgaccat tctcggtgtt tttgaaataa tttatgcggc taaactctta
    31741 agcgcgcagc ctgagccgac aaagccatca cagacgatcg cgatttttca aattctgatg
    31801 atcctttacg gcaatgtctt ttctgtggtg gtggggatcc ttgctttgat cttttacaac
    31861 gaccaaaacg tgaaagatta tttttccaga ttgaacgggg tgcaggtgac cactccagac
    31921 cccatgcccg tttctccgct ccgtgttgag cctgaacctc tgccagagat ctcgccagcc
    31981 gaacccgttg tccctgagat tcctgccctc gaagcagaag cagacccacc cgtcaagccg
    32041 aagcgtacgc gcaaatcatc caccggtaaa taacttgaaa atgagccgcc ctttcaaaaa
    32101 ggcggctttc tttatccaaa ttcaggatgt tttacacctt gactagcatt cccctcactc
    32161 ataaaatgga agcataattc aacggatgtt tgggaagaga ctcaacagta gcgtcgactt
    32221 cagtcggcat tgtagttgag cgccgaaggt gcaaatctgg caaacaggcg aaaccagatg
    32281 aaactctcag gcaaaaggac cctgcttccg atgcaactct ggaaaatccc ccacgggata
    32341 ccgacggtgt aaagtttggt tggtcaaatg gtcaatagtt gaatagtctg ctggtcattg
    32401 cgacaaagcg acgaatagac tagggacaaa gcgaccagcc gactaatctc tcaggttcca
    32461 ttacagagga cgcacgattc tacaatcgtg cgtccttttt tgattcgctg aaaaaaatat
    32521 ttcacaaagg agaaattgac aatgaatgcc ccttccaact taaagtacac caaatctgat
    32581 gaatggttcg atcctgccag tggtgcggca ggaattaccg attacgccca gaatcagctt
    32641 tctgatattg tcttcgttga attgcttgtg gatgaaggcg aaacagtgga agttggcaag
    32701 cccattgctt ccgttgaatc tgtgaaggct tcggctgaaa tttacgcccc tgcttcgggt
    32761 accgtcagcg ccgtgaacaa aggcctttcc gaaaaacccg aaaccctcaa ttccgatccg
    32821 tttggcgaag gctggatgat caagatcgct ggcggctctg ccggtgaagt gatggactcc
    32881 gctgcctaca ccacttattg tgacggacgg gctcattaat tagttgacgg ttgatggtgg
    32941 gtggttgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc ataaagaggc
    33061 accatgacct acatcccaat ttcccccaaa gaacgggatg cgatgctcaa aacggtcggc
    33121 gtcaaatcac tggatgattt gttcgaagcc attccgcaaa aacatcgttt cccagagttg
    33181 gatctgcccc ccgcgctcac tgaaatggaa gctgccacgc tgctcagtga aatggcgcag
    33241 accaatgaaa atgtacgcga gcatatgatc tccttcctcg gcgcaggtgc atacaatcat
    33301 tatgtccctt ctgttgtgga tcacatgctg cgacgcggcg aattctatac cgcctacact
    33361 ccataccagc ctgaaatttc acaaggcacc ctgcaggcga tttttgaata tcaatcgctg
    33421 atgaccaacc tcactggcat ggaagtgtcg aatgcttcac actacgatgg cgccaccgcc
    33481 accgcagaag cagtgaattt ggcttatgct caattccgcg gcaagcgcaa aaagatagtc
    33541 atgtcgccca cggtacaccc gcaataccgc gaagtcatcc gcacgtattc acaaggtatg
    33601 ggcctggaag aagccgggga cgatgtagca gccgacctcg aagctggccc cgaggagttg
    33661 atgtcgctcg tggatgacaa gaccatgctc gtcgtggtgc aatatcccga tttctttggc
    33721 cgcatccacg attacacaaa attgatcgaa gacgcgcacg ccaaaggcgc actcgtttgc
    33781 atcgttgcga atccaaccgc attgctcatg cttaagacac ctgcctccat gggcgcggat
    33841 attgtggttg gtgaagcgca gccgttcggc attccgcttt ggtttggcgg accctacctc
    33901 ggctttttca ccaccaaaaa atcatacgtt cacaaaatgg ctggccgcct gatcggtgaa
    33961 accgtggatg cccgtggaca acgctcctac gtgctcaccc tcaccgcgcg cgaacaacac
    34021 atcaaacgcg agcgtgccac ttccaacatc tgcaccaatc aaggtttgct tgcactcgcg
    34081 tccgccgttt acatgtccac gcttggcaag accggcttgc agcaggttgc aaatctttgc
    34141 tatcaaaaag cccactacgc cgcgagcgaa ctgagcaaga ttgacggttt tggtctgtgc
    34201 ttcaccgaac cgttcttcca tgagtttgtt ttgtgttgtc cgaaacctgc cagcgaagtc
    34261 aacgattacc tgctagataa cggcatcttc ggcggctacg atctcggcaa agattatccc
    34321 tcgctcaaga atcaccttct ggttgccgtc accgaaatga acagcaaaca ggaaattgac
    34381 accctcgtgg aagtcttaaa ggagatggaa taatggctac cgttgttgaa cctaccatct
    34441 ttgaactatc ctccccaggg cgactcggcg taaccatgcc cgcttcagac gtacccgaaa
    34501 ctgccctgcc tcccaatcat ttactgcgct ctgacctgcc cctgcccgaa cttgccgaag
    34561 tggatgttgt ccgccactat atgcggctca gcaaattcaa ctacagcgtg gacgatggct
    34621 tttatccttt gggttcctgc accatgaaat acaacccgaa gatcaatgaa gatacctgcc
    34681 gtttgccggg ctttctgttt acgcatcctt tgcagcccat cgaaactgtg caaggcaacc
    34741 tcgcattgat gttcgaaatg caggaatggc tcaaagagat cagcggattt gctggcgtca
    34801 cccttcaacc tgctgcaggc gcgcatggag aattcaccgg cgtgttgatg atggcggctt
    34861 atcacaaggc gcgcaacgat accaaacgca ccaaaatgct catccccgat gccgcgcatg
    34921 gcacaaaccc tgcatccgtt ggcatgttgg gtatgagcgt ggtcaccatc ccatccgatt
    34981 cgcgcggcaa tgtggacctc aaagccctcg aagccgcctg cgacgatacc gtcgtaggga
    35041 tgatgctcac caaccccaac accctcggca tgttcgatga gaatatcgaa caggttgtga
    35101 agatggtgaa ggattgtggc ggcctgatgt atggcgatgg ggcaaacctg aacgccctgc
    35161 tcggcattgt ccgtcccggt gacctcggct tcgatgtgat gcatttcaac ctgcacaaga
    35221 ccttctcggt gccgcacggc ggcggtggac caggctcagg cccggtcggt gtaagccagc
    35281 gcctggttga ttatctgcct tccccgctcg tcggaatcat cgaagaaggc gatgacgacc
    35341 ttgaaccgct gtacggcttt gtcacaccca aacacagcat tggacgcatg aaggctttcc
    35401 acggtcattt cggcggtgga cttgtccgcg cttacaccta tattgccatg cacggccccg
    35461 atgggttgaa ggatgtttcg caatacgcgg tgctcaatgc caattacctg caagcccgcc
    35521 tgcgcgatac ctacaagatt ccgtacgatc ggatctgtat gcatgaattc gtcatggaag
    35581 gacagttcga aggcagtgat atccgcgcgt tggacatctc gaaacgcctg atggattaca
    35641 aattccaccc gccgacgaat tacttcccgc tcatcgtcca tgaagcgttg atgatcgagc
    35701 cgaccgaaac cgaaaacaaa gacaccctcg atcgttttgc cgatgcgctc atcgccatcg
    35761 cagaagaggc caaatcccag cccgatctgc tgcaccacgc tccgcacaat accccgttcg
    35821 gcagaatgga cgaggtcaaa gcggctcgcg aattggtcct ctgctgttgg ctgcctgaga
    35881 attacgaaac gttgaaataa gaatccccct ccctgggctg ctctttaaaa cgggcaccac
    35941 ctgcacgaaa ttacagggtt ataatacgcg gcaaccccgc acaaaagaac atccagcata
    36001 gacctgctgg atgttcttta taaccgccca aggaggagca atgctgaata aactgttatt
    36061 tcatcgtttt ccaaaagcca agagctggct cacgcgcata cagtacgaac tgatctccac
    36121 cctggatagg gacaagaacg ccattttcat gaactttggc tatgctcccc attcacatgc
    36181 gtatgaaccg gttgcgcttc ccaaggaaga ggaaattcac cgatacccgc tgcaactcta
    36241 tcatcatgtg gcaaggcata tcgactggaa aaatgccgat gcgttggagg tcagctccgg
    36301 gcggggcgga ggcgcgcact ttatcatgcg ccacttcaag ccgcgctcct acaccggggt
    36361 ggatttttcc acacgtgctg ttgacttctg ccgaagacaa tataagttgg atggactcaa
    36421 attccaccac ggaaatgccg aagcgttgac ctttccggat aactccttcg atgttgtcgt
    36481 caatgttgaa gcttcgttgt attatcccaa tatctcaaaa ttttttgaac atgtgcggcg
    36541 ggtgttgaaa ccaaacggat attttctcta taccgacctg cgttatgaag aaaaggtcgc
    36601 gatctggcac gcgcagctga aaaacatggg tttgaaactt atcaaggaag aagacatcac
    36661 cgacaacgtc ctgaaagcca tggagttgga ccgtgcacgg cgggttgccc tggtggatcg
    36721 atatgtccct gccattttga gaaagcagtt ctatcatttt gcaggggtgt caccggatga
    36781 gtcggaaacc ctgcctcatc ttgaaaaccg acggtattgg tattttgtcc tgcaaaaggc
    36841 ataacgaaat tcacaaacaa aaggatgcgc cgtttgacgc atcctttttg atttcttaaa
    36901 tcgcttacaa ttgaaaacag aattttccac aaggagtatt tttatgcagc cattaagcga
    36961 agacattcgc caaaagatca ttctgtttca gcagaccgag atcacggaat atcatattta
    37021 caaacgactt gccaaacgca tcaaatccga ggggaatgca aagatctttg accagatcgc
    37081 cgaagacgaa ctgcgtcatt acaacggctg gaaggaatat acgaacgaag aagttcaacc
    37141 gcgctggttc ttcgtctggt tttattatct tgtcagtatt ctgttcggtt tcaccttcgg
    37201 cgtaaaactc atggaaatgg gcgaggaaaa ggcgcaggcc aactatgaag ctgtttccaa
    37261 ggttattcct gaagcaacca aatatcagga tgaagaagac aagcacgaac atcaactcat
    37321 tgaaatgctc gatgaagaac gcctgcaata tgcaggctcg gtggtcctcg gcctgaatga
    37381 tgcgcttgtg gagttgaccg gcgccctggc cgggctcacg cttgccctgc aaaatgtgaa
    37441 gttgatcgcc ctttcaggcc tcatcacagg cattgccgct tcgctttcaa tggcagccag
    37501 tgaatatcta tccacccgtt cagaagagac aaacaagcac cccgtccgtg ctgcggttta
    37561 tacgggcatt gcctacatca tcaccgtgac cctgctcatt ttgccgtatc tccttttcga
    37621 aaactattac ctcgatctgg tcatggcgct taccaccgcg gtggtcatca ttgccgtgtt
    37681 caactattac atctcggttg ccaaaggcga atcgttccgt gagcgctttg tggagatggc
    37741 tgggcttagt ttaagtgtgg cggccttctc gtttgtcatt ggctatttca ttcgtcaatg
    37801 gcttgggatc gaaatctaat ttaccctgag tgaggcggca tgaagattct atccgtggat
    37861 attggcacag gcacgcagga tatttttctt tacgattcga atctcgatat cgagaacgga
    37921 ttcaagctcg ttttgccatc tccgaccatg atggttcacc gccgtctcaa acaggcgctt
    37981 cacacgcgga ccccgatcct gctcagcgga tatcaaatgg gcggcggccc ctcggcatgg
    38041 gccatcgaag aaatggcgcg tgcgggcatc cccgtgtaca tgacgcccga tgctgcaacg
    38101 acattgaatg acgagttgga caaggtggag aaattgggga tcaagatcgt ttcggaggat
    38161 gaggtggatg gtttaagggg gaaggttgaa agcttggagt tgagagattt tgattttggg
    38221 ttgatttcaa aaacatttgc tgattacggt gtttcactga gtgatctatc ggcaattgct
    38281 gtggctgttt tcgatcatgg caatgcgcct gcgggagtct cggacaggca gttcaggttc
    38341 gattatctca atgagcggat ccgcgcaaag aacgccctct cctcttttgc ctttctttct
    38401 tccgatattc ccaggatcat gacccgcctg caatcggtgg cggattcggc gcacgggctg
    38461 ccctgtccgc tggtggtgat ggatacagcc cccgccgccg tgctgggcgc g
//
LOCUS       DAOQJC010000022        37867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_463_length_37867_cov_10.766503, whole genome shotgun sequence.
ACCESSION   DAOQJC010000022 DAOQJC010000000
VERSION     DAOQJC010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 37867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37867
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_463_length_37867_cov_10.766503"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /gene="mraW"
                     /locus_tag="PLE39_04965"
     CDS             <1..192
                     /gene="mraW"
                     /locus_tag="PLE39_04965"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HNE67708.1"
                     /translation="RIVKEYFREQSKDIVNPPYERIYEEERKAVVKIINKKPIVASDE
                     EIKDNPRSRSAKLRVVEKL"
     gene            210..683
                     /locus_tag="PLE39_04970"
     CDS             210..683
                     /locus_tag="PLE39_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67709.1"
                     /translation="MNIPNVNHYIHAYKIAPWRVQRQWIGNALLMVVALAMISTLYLD
                     VTSQAAIAGREIQDLTSSIIVSQQVSGDLQTHLASLTSASVMRQRALELGFRPMEAEE
                     VEYLVVPGYVTLEPDILSSAPLPQLSALTFPPEYNQSLLDWMDNRITSSASRGTQ"
     gene            680..2428
                     /locus_tag="PLE39_04975"
     CDS             680..2428
                     /locus_tag="PLE39_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNE67710.1"
                     /translation="MRQQYARRAQVLTFVMGLIALAIVVQMTRIQNSAEAAVFRQQAS
                     NYAYELRTFYPDRGEIYDRNGHLLAGNKSVYELGVDLSTVKDPHAIAFALSSELGESY
                     DRIYDMIINPPDGLSYVVVADYVESAQAIRLQELKKALQEQAPEGTLGGLTGLQFKSH
                     PQRSYPEFSLGSNIIGFVNREGKGYFGIEEKYDTLLAGNPVQVLVPTDPNKAFEIPRV
                     PNGTTLVLTINRDLQAAAEEILDRALIEHGAQGGTIAIMDPRNGELLAMAVTPRMDLN
                     EFWNYGAIYDNASDFNPAISMPYEPGSVVKILTMAAALDTGTVTPETTYLDTGSILVG
                     GATIQNWDQEPWGVQDMTGCLQHSLNVCMATLSTQMGAGNFYSYMDNFGLGQLTGVDL
                     AGEAPGRLKIPGDADWYPVDLGTNSFGQGLTATPIQMMMAASAIANHGRVVTPHALYA
                     MMRDGRQYNVPAQFAGTPISQQTADILSGMLAVSLESEGSLALVPGYRVAGKTGTAQI
                     PVNGFYDSTQTNASFIGWGPIDDPRFMIYVWLERPSSSIWGSETAAPVFAEMAQKTVL
                     LMDIPPDSIRQQLVVK"
     gene            2430..3845
                     /gene="murF"
                     /locus_tag="PLE39_04980"
     CDS             2430..3845
                     /gene="murF"
                     /locus_tag="PLE39_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNE67711.1"
                     /translation="MLTLADALEAITSVRIANANAVITEAAIDSRQVIPASLFVAIPG
                     EKMDGHDFIADAFKRGASFALTQKEMPESFRTLDLRNGLTAESPLDLSTPLCLRVDNT
                     ISALQQIARFWRRKLDLRVVGVTGSVGKSTTKETIAEVLGTRYRTLRNPGNLNNEIGL
                     PLTILKLGSGYERAVLEMGFYVPGEIQFLCDIAQPQVGVVTNVGTVHAERAGSQEAIA
                     RGKSELIQALPADGVAILNFDDPWVRKMEEKSKARVFFYGLSPEADLWADGIEGLGLD
                     GIRFRLHYKGETVHSHIPMIGRHSVHTALRATAVGLNDGMTWQEIFEGLLQGHAQLRL
                     VAVRSNTGALILDDTYNASPESMLAALNLLDEMDGRKIAVLGDMLELGPYEKQGHEMV
                     GMRAAQVARVMLTLGPRAHMIADAARRTGMKSANILEFENSEPIIDWLNKNLTSNDAV
                     LIKGSHGLRMDRITAALETQS"
     gene            3842..4846
                     /gene="mraY"
                     /locus_tag="PLE39_04985"
     CDS             3842..4846
                     /gene="mraY"
                     /locus_tag="PLE39_04985"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNE67712.1"
                     /translation="MRQIALSLSLAGLAFVMTAIWGGPLLRILRHYKIGKIIRVEEPG
                     HHGVKMGTPTMGGVMFIFPVLLFSGLLNAVALIGGRSDVVGVGRSILLPMAVMAGFAV
                     LGAVDDWEGIRGKRKGDGMRARTKFMFQVILALAAAYVLKYQMDAPDMYFPGLFFEVE
                     LGWWYVPIAAFIIVAESNAVNFTDGLDGLAGLIAATAIAAFGGIALMQEQVYLARFCF
                     ILVGALFGFLWFNVHPAELFMGDTGSLSLGAVLAVVALMEGQWLMLLVIGIIPLSEML
                     SVVIQIGYFKWTKGKRFFKMAPIHLHFELLGWSETQVVQRFWLIGLMAALIGIGLSQV
                     "
     gene            4919..6325
                     /gene="murD"
                     /locus_tag="PLE39_04990"
     CDS             4919..6325
                     /gene="murD"
                     /locus_tag="PLE39_04990"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNE67713.1"
                     /translation="MTNWNSTRTLILGAARQGIALARWLSRHGSKVTLSDSRSLEQLA
                     SARESLADTDVTWAAGGHPLELLDATDVLCLSGGVPLTLPIVQEAVKRGIPLSNDTQI
                     FLEVVPCKTIGITGSAGKTTTTTLVGKMAVASYKLQVEGNLQHSNNVFVGGNIGDPLI
                     NYVDDMTADDLAILEISSFQLEQMTISPNIAAILNITPNHLDRHGTMEAYTNAKARIL
                     EFQTEKDTAILNHDDKGSWNLRNKVKGKLMTFSLHDLDEGFNGSYLHEDGLLSLRDGY
                     AYLPLMLREKIKLPGLHNVANVLAAFTIGHAAGFKLDAMLEVVEEFRGVAHRLEFVRE
                     RNGVRWFNDSIATAPERAMAGIQSFDDPIVLMLGGRDKDLPWDEIAKLIHKRVDHVVL
                     FGEAAELIQKAISAVGGERSVDLRRARHLKEAVALAAEAATAGDVVLFSPGGTSYDEF
                     KDFAERGEAFRKWVQELS"
     gene            6304..7557
                     /locus_tag="PLE39_04995"
     CDS             6304..7557
                     /locus_tag="PLE39_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HNE67714.1"
                     /translation="MGTGTFVNEQTYKKPRFTGGFDMPLLVVVVALIVFGLAMLYSSS
                     WDFSLGAYGDAMYMFNRQLTWLGLGLFSALVLAFFDYHNWRKYVVPAMALTIGLLVVV
                     LLMNEIRFGAKRAILGGSIQPSELAKLVSILYLSVWLYAKRQYLHDISLGLIPLGVIL
                     GVVGGLIYQQPDLSAAATVLMLGGLLFFLAGGDLKQIGVLLVVATVAAWIVASISATG
                     QQRVGDFIAGIKDPLQASYHVRRSFEAIVNGGWFGVGLGQSQSKLTGLPVPPTDSVFA
                     VVVEELGWFGAVGLIVLYGALVWRGMVIARRAPDMLGTLLATGLVTWIAIEALVNMAV
                     MVGLLPFAGNALPFISAGGSNLVATMASLGILFNISRQTGEGTISDDEWRSYSAVVNL
                     RWWNGRRGLSRTRRTQRANQSAPES"
     gene            7442..8551
                     /gene="murG"
                     /locus_tag="PLE39_05000"
     CDS             7442..8551
                     /gene="murG"
                     /locus_tag="PLE39_05000"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNE67715.1"
                     /translation="MTNGGLTVRLLICAGGTGGGVYPALAVHNALTSQRQKVETLWVG
                     GEGGMEEELVNRAGIPFRSIPAAGVHGVGLRALPGNLAKLTRGVQASRRILREFKPDV
                     LFFTGGFVAAPMAVAGRNIPTALYVPDIEPGLALKFLSRFADVVTVTAPDSKKYFNRP
                     ERLVLTGYPLRSDLSNWSREKATQHFGLDPQMPTLLVTGGSKGARSINMAVLKHLHEL
                     LGAAQVIHITGSLDWPVIEKAAQTLPADLQSRYHAMPYSHEMGAALAAADLVLSRAGA
                     SSLGEYPFFGLPAVLVPYPYAWRYQKVNADFLVEKNAAVLLQDELLEENLPSVVKGLL
                     LNKTKLESMRAAMQALSHPDAAGSIASQLVKLAGA"
     gene            8560..9084
                     /locus_tag="PLE39_05005"
     CDS             8560..9084
                     /locus_tag="PLE39_05005"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNE67716.1"
                     /translation="MSVVYIFWMYIILFGIVGAMRGWAKELMVIFSVITALAVNLLLE
                     KYIPLVRDLDKASSSMFWIRSLILITLVFFGYQTVNLTRFSGKAAREKLQDGMFGAVL
                     GGFNGYLVAGSILFYNHMALYPYSNIISPATDPAIAQAIETMMKYMPPRFLGEPSIYF
                     AVIIVLVFIIVVYI"
     gene            9155..10540
                     /gene="murC"
                     /locus_tag="PLE39_05010"
     CDS             9155..10540
                     /gene="murC"
                     /locus_tag="PLE39_05010"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNE67717.1"
                     /translation="MKRVHFIGIGGSGLSAIARLLKESGYEVTGSDRTLSPFAVDLQN
                     NGVSIYVGHHPRNVSGADIVIRSSAIPDENVEVEAAKRAGVPVYKRSDFLGQLMSEKT
                     GIAVAGTHGKTTTTAMIAWTLTALKRDPSFIVGGTLNNLKVNARAGKGSPFVIEADEY
                     DRMFLGLKPQIAVVTNLEHDHPDCYPTFADMYSAFQGFVDLLPEDGTLIVCAEDEGAV
                     SLLSYARKKGLSVISYSVQSELTINSPQWMQARTPKPNTRGGFDFSAMTNMGTESASV
                     VNVSLQVPGEHNVRNALAALSVMATLGLPLQEAADALGEFTGTGRRFEIRGEQKGVIV
                     IDDYAHHPTEIRATLAGAKARYPDRRIWAVWQPHTYSRTQTLFFEFSRAFSDADEVLV
                     TEVYASREAKQEFSSAEVVGSMPHASARYSGSLEDTTAYLLKNLRSGDVLLVLSAGDA
                     DQISTNVLKNL"
     gene            10548..10769
                     /locus_tag="PLE39_05015"
     CDS             10548..10769
                     /locus_tag="PLE39_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67718.1"
                     /translation="MLENHEKQMAHLYEVIALPPLKMALFHAVDEQLTKAALINATRK
                     LSAIPVLGSENVGKQSLEKIIGHLEKKKL"
     gene            10772..11701
                     /gene="murB"
                     /locus_tag="PLE39_05020"
     CDS             10772..11701
                     /gene="murB"
                     /locus_tag="PLE39_05020"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNE67719.1"
                     /translation="MDMARSALHVDVLRQIFGDAVQENISLAPYTSARIGGPADVLLT
                     VKSADEFAEAMKVIWENDFPYYILGGGSNVLVSDLGLRGVVVLNRAKEVRFEGGDQPT
                     VWCEAGVIFSNLANRCASKGLAGLEWAATVPGTVGGAVYGNAGAFGGDVSMNLIWADV
                     LTKNGRERMSVEQMGYGYRTSVLKRGEIHAIVLSALLRLKNSAKEEVSVKIEQFSERR
                     KATQPPGASMGSMFKNPEGDHAGRLIEAAGLKGVRIGNAEISTLHGNFFINHGETRAK
                     DILALIRLTQKTVREKFGVQLELEVELVGDWNE"
     gene            11730..12842
                     /locus_tag="PLE39_05025"
     CDS             11730..12842
                     /locus_tag="PLE39_05025"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNE67720.1"
                     /translation="MKKLRVAVLFGGRSGEHDVSLMSARSVLNALDAERYEVIQIGIT
                     LDGDWFTGPNALDAFENGSVDGLDRVVPPWEPSYQSLYVIRSTKLGEKLETLSGVDVF
                     FPVLHGPFGEDGTIQGLLELADVAYVGAGVAGSSVGMDKGIFKDVMRANGIPIVDSIL
                     ILRDELEKDMNAVIAKVEQMGAYPFFAKPANLGSSVGISKCNSRSDLGEGLMEAARYD
                     RRIIVERGVGNVREIEVSVLGNEYPQTSVCGEVQPSREFYSYESKYIDGTSGLIIPAI
                     LPADVSDQIRDYAARAYKAIDCAGMARADFFFDNDMKKIYLNELNTLPGFTSISMYPK
                     LWQASGLNYPQLVDRLIELAFERKAQRDHTERRRTA"
     gene            12839..13867
                     /locus_tag="PLE39_05030"
     CDS             12839..13867
                     /locus_tag="PLE39_05030"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNE67721.1"
                     /translation="MSERQRPTRAELARQRRNQRARKELELTTKRALKPMQSVTSRVP
                     EIVVAPKLTRQSAPRRRFNIALGLPEIHLHKPQFHMPRIRPSWRLTSFVLVVLLGVVI
                     YLSLTLPYFHVPNITLLGGGRLSREEIEAVLAVNGQSIFTVQPDELEVRLRMNYPELS
                     SVDIHAYLPNHVYVTVVEREPVILWQQGGGFTWIDASGVAFRPRGMVATLVPVNGLST
                     PPAGQPATEDPLSPMPYVSKELVDSILILAPNVPAGATMFYDAANGLGWEDERGWKAY
                     FGISAHDMPLKIRVYQSLVASLTSRGTVPVFISVVYPDAPFYRMADLELDATDAASED
                     AIIEDGQQ"
     gene            13870..15114
                     /gene="ftsA"
                     /locus_tag="PLE39_05035"
     CDS             13870..15114
                     /gene="ftsA"
                     /locus_tag="PLE39_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNE67722.1"
                     /translation="MDEIVVGVDVGTTKICTLVGRVEDAKTIRILGVGIEPSDGIRKG
                     IIVDLSAASQAIKRSVEKAENTSGLEITTALVSLAGAHVSSVNSRGASGIPGGIIDAM
                     DIARALEQAQAVAIPHDREIVHVIQRGITVDGQEGIRTPIGMHGYKLEVETHIITAAA
                     ATVDNLRQCVGAAGVEIQQFVLNPLASAEVVLTEQERQMGVAVCDIGGGTTDLAIYVN
                     GDVWHTMVLAVGGNHVTQDIAHGLRLPMAQAEEVKKQQGYAIRSDIASDEYFSIRPFG
                     EDNDVKINRQDLAHIIEARIEETFRLILQEVKRSGYDGLLPAGMVLTGGTSALPGIKQ
                     IASEVLGMPVRTAQPENLVGLVDKLGSPAYSTSVGLLRWATTMRDHDVIVTSGNKNRR
                     RSRGESSMNLDSIKNWMKRLLP"
     gene            15241..16407
                     /gene="ftsZ"
                     /locus_tag="PLE39_05040"
     CDS             15241..16407
                     /gene="ftsZ"
                     /locus_tag="PLE39_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNE67723.1"
                     /translation="MDTNKRNPNTEAFARIKVIGVGGAGQNAVNRMMEEGIQGVEFIS
                     ANTDAQALTLSRAPIRVRLGDKLTRGLGAGGDPEIGRKAAEESSDELYNVLKGADMVF
                     VTAGMGGGTGTGAAPVVAQVAKECGALTIGVVTRPFTFEGGKRTQSAESGVAKMKEHA
                     HTLISIPNDRLLQLADKKSSLQDAFRMADEVLHQGIQGISELITIPGLINLDFADVKA
                     IMSEGGAALMAVGRGSGDERAKIAAEQAISSQLLDITIDGARGVLFNVTGGSNMTLFE
                     VNQAAAIIRETAHPDVNMIFGAVIDPEMGDEIRCTVIATGFERTSVPRRALERPAART
                     EKPTAASASALFARSSESVSVSADYKTPGESKPASQPSISNDDLDIPTFLRNRR"
     gene            complement(16489..17541)
                     /locus_tag="PLE39_05045"
     CDS             complement(16489..17541)
                     /locus_tag="PLE39_05045"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNE67724.1"
                     /translation="MKNFLIYGSYGYTGSLIVEQAVKAGMKPLLAGRDENLLRTQAEK
                     FGLEYRAFSMDHSAALDAALLEVNAVLHCAGPFVLTYRQMVEACIRTRRHYVDISGEI
                     EGFEALAAMDEEAKRVGIMLLPGGGFDVVPSDCLIAHVAKKLANPTHLEIYIKSIGSG
                     VSRGTARSGIENMQRQGRIRRNGEIVSVPNVWDSKQVDFGRGPSRVVSVGWGDVSTAY
                     HSTGIPNVTAYMGFPAAMVNGMRLTRHLGFLLYNRAAKDFIKWLIGVFLKTGPSRQQN
                     ENGFSLMIAEATNGKQTVRAKLRTPEAYYLTALTAVEIMTRITSEDFKPGFQTPSKAY
                     GSDFILQFEGVTRDDL"
     gene            complement(17538..18350)
                     /locus_tag="PLE39_05050"
     CDS             complement(17538..18350)
                     /locus_tag="PLE39_05050"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HNE67725.1"
                     /translation="MNWRLLYTPPSSGAWNMAVDESILEHIHRGESLPTLRLYSWNPP
                     CLSLGHAQSFADVDVERLRANGWEVVRRVTGGRAILHTDELTYSVTGGADEPVLAGGV
                     LESYNRLAQALLFAIRELGLPVEIKEHISEPATLNLNPVCFEVPSTYEITVEGKKLIG
                     SAQSRKKEGVLQHGSLPLTGDLTRICQALVFRDETARASAAQRLLARATTVESTLGVN
                     PDWETAAQAFVKGFESQLGIHFQRGELSASESERAEELIQEKYANPSWTERS"
     gene            complement(18405..19340)
                     /locus_tag="PLE39_05055"
     CDS             complement(18405..19340)
                     /locus_tag="PLE39_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNE67726.1"
                     /translation="MTDLHPLAGVYAAAVTPLLDSPAKGSKLDSNFDLEAVPALLRHL
                     AASGCHGALLFGTTGEGPSFSPKEREVLLRSVRVYRQQLPGFKLLAGTGTPSLSETIE
                     LTKLTFDLGYDGVVVLPPYYFRKAADEGLFKWFSELITKAVPAHKYLLGYHIPSAAGI
                     GFSLDLIARLKDAFPNQFAGIKDSSHDEAFSAAVGQRFGRDLLVLNGTDSHFHHALKN
                     HAQGAITAMANLIGSDLRDIWDLYNEGGDPSEIQVRVAKQRHALEKYTPFPPTLKALL
                     HKLHSLPRWTVRPPLEDISPELEDQILQGWESLQV"
     gene            complement(19343..20200)
                     /locus_tag="PLE39_05060"
     CDS             complement(19343..20200)
                     /locus_tag="PLE39_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67727.1"
                     /translation="MSDELTSRVRADFSRARFKSFINQAIAVLKGQRPTTLLSYDDVK
                     EKLHIGGPIYRGVKTIRVEQIAGSLNRYHEFDRAFLPREDQLASRWQKVDRAFYQDVN
                     LPPVVLYKVGEVYFVVDGHHRVSVAREQGQEFIEAEIRECATRVNLTPNIQPEDLEIL
                     GTKVHFLERSSLDHIRPEANIKLTIPDGFERMLEHIAVHHYFMGLDLKRDVTEEEAVA
                     HWYDTVYMPIIDVVRKSDILKNFPDKTEGDLYLWTLDHQHYLSKEKGQPLQPPHEAAQ
                     RFIRKKKKK"
     gene            complement(20203..20829)
                     /locus_tag="PLE39_05065"
     CDS             complement(20203..20829)
                     /locus_tag="PLE39_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNE67728.1"
                     /translation="MKILAVSDQAIERLYTLSASGHFQDVELILGCGDMPFEYLEYLV
                     TMLNVPLFYVPGNHDPQHNPRQESVRPEGGVNLDLKTVRHKKFLIGGFGGSIRYRPDG
                     VNQYSQQEAYYRAFSMLPRLLAYRIQYGRPLDILISHSPPFGIHDDSDPAHQGLKTLN
                     WLLRITKPRYHFHGHTHLYRRNLENVETNIGLTKVINVYPYKTIEVSH"
     gene            21058..22854
                     /locus_tag="PLE39_05070"
     CDS             21058..22854
                     /locus_tag="PLE39_05070"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNE67729.1"
                     /translation="MDRYNIQDVIGVGGMGSVYRARDLHFPNVVKLVAVKEMINSAPD
                     PLVRQTIVQNFEREANLLATLNHPAIPRIYDYFSLDDRSYLVLEFIHGKDLEAVIGDS
                     NGFLPEDQIISWAIQLCDVLAYIHGHKPDPIIFRDMKPSNVMINHNGDVVLVDFGIAK
                     TFQSGQKGTMIGTEGYSPPEQYRGEATPLADIYSLGATLHHAITRRDPRLEPPFSFAE
                     RPIRRINPNVSLEFEAVVNTALQYNPGDRFSSTAAMKDALLNVARKTGTLEKVAAALP
                     VSSGGVKPLWTFKCEDEIRGTPALHQGMLFIGCYDNNLYALNAADGQFQWKYATEGGI
                     VSRPTVFDNNVIFGSEDHRLHVVSIRSGKVVWTYYTEGEIYSSPRIADGHIFFGSDDQ
                     DLHAVNITSGRAKWKFATDSPIHSTPLVANEMIFFGNESGGFYSVDFRGEQKWRFQSK
                     RAVTSSPVIKGQAIYFASVDGTLYALDSKNGWPIWKFRMGKGSISSPAIADDFIFVGA
                     ADGFLYCVDTRNAKEVWRFRTENQVSGSPVIYKDSLYCGSVDGSLYCLEYRTGRLRWK
                     FETAAAITGSPIVFDDIVYFGSTDHQVYALFA"
     gene            22867..23784
                     /locus_tag="PLE39_05075"
     CDS             22867..23784
                     /locus_tag="PLE39_05075"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNE67730.1"
                     /translation="MADFFRKLFGTKPTEEPAKPKQVDMATTAPLSDQQINSIIANQY
                     VRYETKQVIASVGQSVGKQREHNEDSLLAITSTIAGSTESIPFGLYIVADGMGGHQFG
                     EVASNAAVRIMGGNITKKFHSYLYNLPTQALQESLQEVMESSVMEAHQYVQREAPGSG
                     TTVTAALVLGQQVTIAHVGDSRAYSIYPDGRIEPITRDHSLVKRLEELGHLSKDEAAN
                     FPHRNVLIRALGQGESLEADVFTVPFPQAGYLMLCSDGLWGVISEKDIYRSIAEAPNL
                     HRACQNMVEAANLAGGPDNITVILVQMVG"
     gene            23787..25268
                     /locus_tag="PLE39_05080"
     CDS             23787..25268
                     /locus_tag="PLE39_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNE67731.1"
                     /translation="MASSKSDHYATLGVFRDASPEEIKRAYFEAAQRLHPDKNVAAGE
                     TELFLEIQQAYEVLSNPKRKNLYDATLPPEINVNTIVKHDVTFSRPNLVKIGEPQLLY
                     ALLEISPREDKESLPAPPLNICLVLDRSTSMQGDKMDMVKATAVQLLRSLRPEDVFSV
                     VAFSDRAEVIVPAAFNVDKKKQEARIQMMQPSGATEIFNGLKAGLQEVRRTIDPSRVN
                     HIILLTDGQTYGDEQECLKLAEEAAAQNIGITGMGIGHEWNDIFLDALASKTGGSSAY
                     ISKPKDIQQLLVDKFNALISTYADEVILDFKPRDDIKIKYAFRLQPEGGSIAIETPMR
                     LGPILRDAPLHVLIEFIVEPSALDEEVVTVLNGMLKVSITARAAPVPPIRINLKRDVH
                     SNPSAEPPPTRILSALSRLTMYRMQERARTAADAGQYDEAARHLHNLATHLLSKGENS
                     LAKTALFEAENLEKMHAWSASGTKDIKYHTRALLMGGSKEKVK"
     gene            25265..25789
                     /locus_tag="PLE39_05085"
     CDS             25265..25789
                     /locus_tag="PLE39_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNE67732.1"
                     /translation="MIICSNCKNTNLDGAMFCAECGSQLVGKDTLITQNISTKQFEKA
                     RESKPARPNDTYQPFDGSDAWGSLHLLDTGQVLPLSNKNEFTMGRISEGQPIMPDIDL
                     SPYQAYAAGVSRLHAVIKREAGTGRIIFIDLGSANGTYINGKRLTPNVEQVLVHGDIV
                     ALGKLKLQILVKSG"
     gene            25810..26052
                     /locus_tag="PLE39_05090"
     CDS             25810..26052
                     /locus_tag="PLE39_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67733.1"
                     /translation="MSYTVILHISGETSVAGEIEELPKPTDTIIMVMNPRQKDGKDLH
                     YIDNNVTKVIWPLAKLSFIEILEGTGEEKIISFVRE"
     gene            26060..26779
                     /locus_tag="PLE39_05095"
     CDS             26060..26779
                     /locus_tag="PLE39_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE67734.1"
                     /translation="MSDSLERRRILVVDDEERMVRFIRMNLEHDGFQVSEAFNGKQAI
                     QKLRDVTPDLILLDVMMPDLDGFEVLETIREITTVPVIMLTAKGEEDDRVRGLEGGAD
                     DYVTKPFSPRELVSRVKAVLRRTEGATGSMHGLLEIDKRLKIDFDRREIWLEGKLVKL
                     RPTEYRLLFHLVQNAGWVVSHDQLLQKVWGYEYRDEPHYVRLYINYLRQKLEKDPAEP
                     KYILTERGVGYRFVDYKRSKE"
     gene            27005..27448
                     /locus_tag="PLE39_05100"
     CDS             27005..27448
                     /locus_tag="PLE39_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNE67735.1"
                     /translation="MSNLIRWEPAREMMTLREAMDRLFDDAFTRPLSIAGNGWAVPAV
                     DMYQTDNEVVVKAALPGMKADDVQLNVTGEVLTIKGEIKQKDELKEKAYHLREQRWGS
                     FERSVILPTEVVADKAKADFENGILTITLPKAEEAKPKSISIKAK"
     gene            27549..28796
                     /locus_tag="PLE39_05105"
     CDS             27549..28796
                     /locus_tag="PLE39_05105"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HNE67736.1"
                     /translation="MTNSFSLLRINPDRMQDTFNQLALIGSTGDGGVNRPTFSEAHLA
                     ARKWFKEQIEDSGLEFRTDGAGNHSAFLTCQRSNVPTLLLGSHLDSVPKGGRFDGALG
                     VMAALEVLRTVKESRINLRLNLEAIDFTDEEGTHLSLFGSSAISGHLHPEDLKNPYSG
                     RENFTAGLARAGLTEASLFQAKRAKESLAGYLELHIEQGKRLERAKIDIGIVSAIVGY
                     GSYRMSYIGRADHAGSTTMEDRLDAGQGVAAFILAVRETVMRDFPNCVANVGKIEFAP
                     GASNIVPARADFLLEFRSPDENQLAELDATLMMLAKEMAEQFGLQLVAEPLGKHLPNL
                     MSTEIQDAFKNSCDDLGLSSTVLASGAIHDAQSLVDVCPIGMIFVPSVDGASHAPREF
                     TKWADCVNGANVLLQTVLRLAAG"
     gene            28806..29819
                     /gene="add"
                     /locus_tag="PLE39_05110"
     CDS             28806..29819
                     /gene="add"
                     /locus_tag="PLE39_05110"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNE67737.1"
                     /translation="MKNFDPKYNNIPKTEIHCHLEGAIRTQTIIDVAREYDLSLPSYD
                     VRELDTHVKVFDQMRNLEAVLEAFGIFQNSITSPAVFERISWELFEDCAKQNIKLFEV
                     RFSPDWAFHGHNLDWDACLDGLLRAKERAEKEFDMAIGIIAITSRGMGAESCVKTVDW
                     AIRHKKHIQAVDLADGELLYPMSDFVKPILKAKEAGLKVTIHSGEDTPASAVWDTIHN
                     FQPDRIGHGIHSIEDMKVVDLIKERGITLEVNPWSNYLTNSVRTIEEHPLKKLFDLGV
                     RVTINSDDPEVLETNVNNEYRIAHEVLGMSMEDIATCNRYAFESSFVDEKVKQRIWQK
                     YFM"
     gene            complement(29878..31305)
                     /locus_tag="PLE39_05115"
     CDS             complement(29878..31305)
                     /locus_tag="PLE39_05115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE67738.1"
                     /translation="MSNQVEHYKSLFEYAPISLWEEDYSGIKLFFEGLRLGGVTDLKA
                     HFAHHPNDVDACMRRIKVTHVNLKTLEMFGARSVEELIANLHKIFRDEMRHHFQTELL
                     ALWNGETNWSGEGINYTLKDKPLNIRLRWKILPECCEKWECVMVAIEDITPLKQAEKR
                     FQNLFTYAPISLWEENYQGIREMFDTLRAQGVTDLKTHLLQTPELVGQFMNKIAVTDI
                     NLKTLELYGASSKEELLANLDRVFRDEMSAHFADELLDMWSGKTTYEREGINYSLSGE
                     PFNVHLDWRLMPGHEDDFKWVMVAVQDITARKKAEEYMRYLGTHDVMTGLYNRAFFED
                     ELLQLASSESEPSFIMIDLDGLKHVNDTLGHQAGDNLIRRAAEVLKTAFAEKATIARI
                     GGDEFAIILKNPEAPQEYLDGIHALVALNNKYYQGPQLQLSMGYATRRKNETLEKTLI
                     RADEAMYQNKESNYKNHPSWQRRKE"
     gene            31553..31723
                     /gene="rpmH"
                     /locus_tag="PLE39_05120"
     CDS             31553..31723
                     /gene="rpmH"
                     /locus_tag="PLE39_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNE67739.1"
                     /translation="MTKRTYQPKIRRRVRVHGFRARMATADGRAVLKRRRLKGRYKLT
                     VKANEHVKKTRW"
     gene            31768..32121
                     /gene="rnpA"
                     /locus_tag="PLE39_05125"
     CDS             31768..32121
                     /gene="rnpA"
                     /locus_tag="PLE39_05125"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNE67740.1"
                     /translation="MQSKFRLSRSEDFKRVRRTGKSYAHPLVVLVAQTTESPARHVRV
                     GVAAGKTTGTAVHRNRAKRLLREAMRPLLTSLASGWDLVLIARPSLVSATLEDTRSAL
                     LALLQRAKIIPTDES"
     gene            32111..32413
                     /gene="yidD"
                     /locus_tag="PLE39_05130"
     CDS             32111..32413
                     /gene="yidD"
                     /locus_tag="PLE39_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNE67741.1"
                     /translation="MNPEPEREFHLHDYSHEPRLRDMPRNLANWPRIAMLALIRLYQM
                     AISPGLPANTCRFYPSCSHYGYQAVYKHGALKGSLMAVWRVLRCNPFNPGGYDPVP"
     gene            32454..33527
                     /locus_tag="PLE39_05135"
     CDS             32454..33527
                     /locus_tag="PLE39_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNE67742.1"
                     /translation="MNTKRLVLISLLVLGSLILSACSGNRTVSNNWHGLAADAEHAYV
                     ASGTHIYAVNMDNGDEAWRFPQEADSKLMFYANPVLTADGQLLIGSAGSSHPFYSLNP
                     ANGKENWVEPFTGAKGAWLASPLIANDTIYAPNTDGYVYVLDLEGKQAADPIQIGGAL
                     WSAPVTDGKFIYIASLDHHLHIIDPSDGSTVDTIDLGGAVPGTPAVAENGVYVGSFAS
                     TVEFIQSNGNHEVVAKTENWIWGSPVLAGETLYYADLTGKVFSFNTTEGSQNWEIALP
                     DAVVAGLLVSGNQIYVATEAGNLIALDLDGNTVWEKPVGGKLYTAPVLSGETILVAPY
                     QAETTLVAYDVDGKQVWPFTPEK"
     gene            33540..34331
                     /locus_tag="PLE39_05140"
     CDS             33540..34331
                     /locus_tag="PLE39_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNE67743.1"
                     /translation="MWDTIIINPMTNLLLWIYDVLGHGPHMFGLAIILFTILIKAVTW
                     PLNASQVKGAQAMQELNNDKEWQEIQKKYAKDREKLAQEQMRIYKEKGINPFASCLPT
                     LIQFPIIIGLYQSITRALSITPFDMLKLARTIYPFQNVENIIPLNSTFLWMDLGRPEG
                     IFQSIIHTSFLPNGFPLLALIVALTTYVQSKLTMPTSSNPNDQSAQMSSMMSIYMPLL
                     LGWFALNFASGIAIYFITSNLLGIIQYAATGRANWSNLLPGSKNK"
     gene            34348..35187
                     /gene="jag"
                     /locus_tag="PLE39_05145"
     CDS             34348..35187
                     /gene="jag"
                     /locus_tag="PLE39_05145"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNE67744.1"
                     /translation="MSGTTLEIIAPTVEEALAQGLAQLGLTADAVSVEVLDSGSKGLF
                     GLGGRQVRVRLTVNPPPVEETHTPAPARQKPADRPKGKPAPAQKAESAPKAQREPKPR
                     TEKKPAPEAPAPVKQRPLSEGSDALLDTTESVISKLIHHLGMKAQVSAHYDESSTEDR
                     RVIQVDVRGDDLSALIGRQAETLNAFQYVASLIVGKETQKWVQLVVDVEGYRSRREKQ
                     VRQIALRMVDQVIKTGRKATLEPMTASERRAIHIELRGHPAVTTESVGEEPHRKVVIL
                     PKE"
     gene            35288..36139
                     /locus_tag="PLE39_05150"
     CDS             35288..36139
                     /locus_tag="PLE39_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNE67745.1"
                     /translation="MLELVSLQPVDYLVVGHAAVDITPTGMQLGGTVSYSSLTARALG
                     MRVGIVTAVGEGAPMQALDGITIVNIPSEHSTTFENIKTDHGRRQVLHHQAAPITLEH
                     IPQVWRSAPIIHLAPIARELDPSLPEKLTGSLLGITPQGWMRQWDENGQVTASAWENR
                     DQALRYAGAVVLSVEDINRDLELVEEMAHQTRILCMTEGELGSVLHWNGDRRRFRPSV
                     MHEVDATGAGDIFAAAFFIRLYATRDPWEAARFATQLAARSVTRVGLNGIPTKDEIEE
                     CLMEVYQ"
     gene            36136..36906
                     /locus_tag="PLE39_05155"
     CDS             36136..36906
                     /locus_tag="PLE39_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNE67746.1"
                     /translation="MTRIYTLVNQKGGVGKTTTTINLGAYLAQLGQRVLVVDLDPQAN
                     ATSCLGVDKLSVQGGTYEALLGDTSIASYVLLNEKLKLSLLPSSPSLAGAEVELVDEL
                     AREVRLRRALEQVASQYDYVLIDCPPSLGLLTVNGLMAAINGVIVPVQCEYLALEGLG
                     QLTQTINRVRSLLFPELNVRGVVLTMFDNRTNLSTDVVSEVNRHFPDQVFKSVIPRSI
                     RLAEAPSYGLPISAYAPTSVGAAAYEALAKELLKGDKN"
     gene            36985..37851
                     /locus_tag="PLE39_05160"
     CDS             36985..37851
                     /locus_tag="PLE39_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNE67747.1"
                     /translation="MAQKTGLGKGLDALIPAGGKTPPASSSGGGTGGVQQVAVEAIKP
                     NPRQPRVHFKEDELAELAASIREHGVIQPIIVTPKADGTYLIIAGERRWQASQKAGLR
                     TVPVITRQANNQELLELALIENVQRADLNSIEEAEAYRQLVEEFGLSHEAVAKRVGKS
                     RVAVTNTLRLLGLADVVKQALVDGTVTEGHARALLALSTQKAQASALQTIINLSFSVR
                     QTEEYVRKLAGQKPIKAKKPNRSADVADVEKRLQNSLGTKVALKHSKKGGTVTIYYYS
                     GEELDALLEKLI"
BASE COUNT         8731 a         9821 c        10105 g         9210 t
ORIGIN      
        1 cgcatcgtca aagagtattt tcgagagcag agcaaagaca tcgtcaatcc gccttacgaa
       61 agaatttatg aagaagaacg taaggctgtt gtaaaaatca tcaataagaa accgattgtg
      121 gcttcggacg aagagatcaa ggataacccg aggtcgcgca gtgcaaaact tagagttgtt
      181 gaaaagttgt agtcagagag agaagagcag tgaacatacc aaacgtaaac cactatatcc
      241 atgcctataa gatcgctccc tggcgtgttc agcgccagtg gatcggtaat gcgctgctta
      301 tggtcgttgc gcttgcgatg atttccaccc tgtatctgga tgtgacctcg caggctgcca
      361 ttgccggccg cgaaatccaa gacctgacct cttcgattat cgtcagccag caggtgagtg
      421 gcgacttgca aacccacctt gcttcgttga cctccgccag tgtcatgcgc cagcgcgcgc
      481 ttgagcttgg tttccgcccg atggaagccg aggaagtgga gtatttggtg gtgcccgggt
      541 atgtcaccct cgaaccggat atcctctctt ctgccccgtt gccgcagtta agcgcgttga
      601 ccttcccccc cgaatacaac caatccctgc tcgattggat ggataaccgc atcacatcct
      661 ccgcttcgcg aggcacccaa tgagacagca gtatgcgcgc cgcgcgcagg tgttgacctt
      721 cgtcatgggg ttgatcgcgc ttgccatcgt cgtgcaaatg acgcgcatcc agaatagcgc
      781 cgaggccgct gtattccgac agcaggcctc gaactatgcc tacgaattac gcaccttcta
      841 tcctgatcgc ggcgagatct acgaccgcaa cgggcatttg ctggcgggca ataaatctgt
      901 gtacgaactc ggcgtggacc tgagcaccgt caaagacccg catgccatag ctttcgcgct
      961 tagttccgaa cttggcgaaa gctatgaccg catctacgat atgatcatca acccgcccga
     1021 cggtctttcc tacgtcgtcg ttgcagatta cgttgaatcg gcacaggcca taagattaca
     1081 ggaattaaag aaggccttgc aggaacaggc tccggaaggc acgctcggcg gcctgaccgg
     1141 tttacagttt aaatctcacc cacagcgcag ttacccggaa ttctcccttg gctccaatat
     1201 cattggcttt gtaaaccgcg aaggcaaggg ctatttcggc atcgaagaaa aatacgatac
     1261 tctgctcgct ggtaaccctg tgcaggtact cgtccccacc gaccccaaca aggcttttga
     1321 aattccgcgc gttcccaacg gcaccaccct ggttctcacc attaaccgtg acctgcaggc
     1381 cgctgccgag gaaatccttg accgtgccct gattgaacat ggcgctcaag gcggcacgat
     1441 cgccatcatg gatccgcgta acggggaatt actggccatg gctgtcactc cgcgtatgga
     1501 tttgaacgag ttttggaatt acggcgccat ttacgacaac gccagcgatt tcaaccccgc
     1561 catttccatg ccatacgaac ccggttctgt cgtcaagatt ttgacaatgg cggctgcgct
     1621 cgataccggt actgtcaccc ccgaaaccac ctacctcgat acaggctcca tccttgtagg
     1681 cggcgccacc attcaaaact gggaccagga accctggggc gtgcaagata tgactggttg
     1741 tttacagcac tcgctcaatg tgtgtatggc caccctctcc acacaaatgg gcgctggcaa
     1801 tttttacagt tacatggata actttggcct tggtcaactc accggcgtgg atctggccgg
     1861 ggaagcaccg ggcaggctca agatccctgg tgatgcggac tggtatcctg ttgatctcgg
     1921 caccaattcc ttcggtcagg gcctcaccgc cacccccatc caaatgatga tggctgcatc
     1981 cgccatcgcc aaccacgggc gcgtcgttac acctcacgca ctctacgcca tgatgcgcga
     2041 tggacgccaa tacaatgtgc cagcccaatt tgcaggcacg cccatttcac aacaaaccgc
     2101 cgacattctt tctggaatgc tggccgtctc actcgaatct gaaggctcac tggctcttgt
     2161 acccgggtat cgggtggcag gcaaaaccgg caccgctcaa atccccgtca acgggtttta
     2221 cgacagcacc caaaccaacg cctccttcat tggctggggg cccattgacg acccccgttt
     2281 catgatctat gtctggctcg aacggccttc ttcatccatt tggggttctg aaaccgccgc
     2341 cccagtcttt gctgaaatgg ctcaaaaaac cgttctgctt atggatattc cgcccgattc
     2401 gatccgtcaa caacttgtag tcaaataaca tgctcacact cgccgacgcc cttgaagcca
     2461 ttacctcggt tcgcatcgcg aacgccaatg ctgtcatcac cgaagcagcc atcgattcgc
     2521 gtcaggtgat ccctgcttcg ctctttgttg ccattcccgg cgaaaaaatg gatgggcacg
     2581 acttcattgc cgatgcattc aagagggggg cctcttttgc tttaacccag aaggaaatgc
     2641 ccgaatcttt tcggaccctc gatctgcgca acggtctaac ggcagaatct ccccttgatc
     2701 tttccactcc cctctgcctg cgcgtggaca ataccatctc cgccttgcag cagattgccc
     2761 gcttctggcg ccgcaaattg gatctgcgcg tggtcggcgt aaccggcagt gtgggcaaat
     2821 ccaccaccaa ggaaacgatc gcagaagtgc ttggcactcg ctaccgcacc ctgcgcaacc
     2881 ccggcaattt gaataacgaa atcggcctgc ctctcaccat tttgaaactt ggttcagggt
     2941 acgaacgcgc cgttctcgaa atggggtttt acgtccccgg cgaaattcaa ttcctgtgcg
     3001 atattgccca gccgcaggtt ggtgtggtga cgaatgtcgg cacggtccat gcagaacgtg
     3061 caggctcaca ggaagccatc gcccgcggaa aaagcgaatt gattcaggca ctgcccgcag
     3121 atggtgtagc cattctcaac ttcgatgatc cctgggttcg caaaatggaa gaaaaatcga
     3181 aagcacgggt ctttttctac ggcctttctc ctgaagccga tttatgggcc gatggcatcg
     3241 aaggccttgg cctggatggc attcgcttcc gcttgcacta caaaggtgaa accgttcatt
     3301 cgcacattcc catgatcggg cgccattcgg ttcacactgc cttgcgtgcc accgcagtgg
     3361 gtttgaatga tggaatgacc tggcaggaga tattcgaagg cctcttgcag ggacatgccc
     3421 agttgcgcct tgtggctgtt cgcagcaata ccggagcttt gattttggac gacacctaca
     3481 atgcctcacc cgaatccatg ctggcagcgc tcaacctgct cgatgaaatg gatggacgaa
     3541 agatcgctgt gttgggcgac atgcttgaat tgggacccta cgagaaacaa ggacacgaaa
     3601 tggttggaat gcgtgccgca caggtggccc gtgtcatgct tacccttggt ccgcgtgcgc
     3661 atatgatcgc agatgctgca cgccgcacag gtatgaagtc tgccaatatt ttggaattcg
     3721 aaaattctga accgatcatc gattggctca acaaaaacct cacctcgaat gatgccgtac
     3781 tgatcaaggg ctcacacggc ctacgcatgg accgcattac cgccgctttg gagactcaat
     3841 cgtgagacag atcgccctat cactcagcct ggcaggcctt gcttttgtaa tgaccgccat
     3901 ttggggtggc cctctgctcc gtattttgcg ccattacaag atcggcaaga tcatccgcgt
     3961 ggaggagcct ggtcaccacg gtgtgaaaat gggaactccc accatgggtg gtgtgatgtt
     4021 cattttcccg gtgttgctgt ttagcggcct gctcaatgct gtggcgttga tcggcggtcg
     4081 ttccgatgtg gtgggggtgg ggcgttccat ccttttgccg atggcggtca tggctggttt
     4141 tgccgtcctc ggcgcggtgg acgattggga aggcattcgc ggtaaacgga aaggtgacgg
     4201 catgcgtgcc cgcaccaagt tcatgtttca agtgattctg gctctggcgg ccgcctatgt
     4261 gttgaaatat caaatggatg cgccggacat gtacttcccc ggtctctttt ttgaagtgga
     4321 acttggctgg tggtatgtgc ccatcgccgc attcatcatt gtggccgaat cgaatgccgt
     4381 caattttacc gatggacttg atgggctggc agggctgatc gccgccactg ccattgccgc
     4441 atttggcggc attgccttga tgcaggaaca ggtctatctt gcgcgttttt gtttcatttt
     4501 ggtcggcgcg ctttttggct ttttatggtt caacgttcat ccagccgaat tgttcatggg
     4561 tgataccggt tcactctcac tcggtgcagt gctggctgta gtggccttga tggaagggca
     4621 gtggctgatg ctgctggtca ttggcattat tccgctcagc gaaatgttga gcgtggtgat
     4681 ccagatcggt tatttcaaat ggacaaaggg caagcgtttt ttcaaaatgg cgcccatcca
     4741 cctgcatttt gaattactcg gctggagcga aacacaggtt gtgcagcgct tctggctcat
     4801 tggcctcatg gccgcattga tcggcatagg attgtcgcaa gtatagaaaa tggtgaatag
     4861 tgaataggcg acgggttttc ttaacctgta accttaaact ttcaaccttc aaaccataat
     4921 gaccaactgg aactcgaccc gcaccctcat tctcggagca gcacggcagg gaatcgccct
     4981 tgcccgctgg ctctcccgcc atggctcaaa ggtgactttg agcgattcgc gttcactgga
     5041 acagcttgcc tctgctcgcg aatctcttgc ggatacggat gtaacttggg cggcgggtgg
     5101 tcatccgctg gaattgctgg acgcgaccga tgtgctttgc ctctccggcg gcgtgccgct
     5161 gacattgccc attgtgcagg aagcagtcaa gcgcggcatt ccgctttcga acgacacgca
     5221 gatattcttg gaagttgttc catgcaaaac gatcggcatc acggggtcgg cgggcaaaac
     5281 caccaccacc acactggttg ggaagatggc ggttgcaagt tacaagttgc aagttgaagg
     5341 caacttgcaa cattcaaata atgtttttgt cggtggcaat atcggcgatc cactcatcaa
     5401 ttatgtggat gatatgaccg ctgacgacct tgccattctt gagatttcat ccttccaatt
     5461 ggagcagatg acgatctcac cgaacatcgc ggctattttg aatatcacgc cgaatcattt
     5521 ggaccgccac ggcacaatgg aagcctacac caatgccaag gcgcgcattt tggaatttca
     5581 gaccgaaaaa gataccgcca tccttaacca cgacgacaaa ggctcgtgga atttacgcaa
     5641 caaggtcaaa ggcaaactga tgaccttcag cctgcatgat ctggacgaag gtttcaatgg
     5701 ctcgtacctg catgaggatg gcttgttgag cctgcgtgat ggctatgcct acctgccttt
     5761 gatgttgcgc gagaagatca aattacccgg ccttcacaat gtagccaatg tgctggccgc
     5821 cttcaccatt gggcatgccg ccggattcaa attggatgcc atgctcgaag ttgttgaaga
     5881 attccgcggt gtggcacaca ggctggaatt tgtccgcgag aggaatgggg tgcgctggtt
     5941 caacgactcg atcgcaactg cgcccgaacg tgccatggct ggcatccagt catttgatga
     6001 tccgatcgtt ttgatgctcg gcggacgtga taaagacctg ccctgggatg aaattgcaaa
     6061 actgattcac aaacgtgtgg atcatgtggt gctcttcggt gaggcggcgg aactgattca
     6121 gaaagccata tcagctgtcg gcggggagcg aagcgttgat cttcgtcgcg ccaggcatct
     6181 taaagaggcg gtggcccttg ctgccgaagc tgccacggca ggagatgtcg tcctgttctc
     6241 accgggcggc acaagttacg atgaattcaa agattttgca gagagaggag aggcctttag
     6301 aaaatgggta caggaacttt cgtaaacgaa cagacttaca aaaagccgcg cttcaccggt
     6361 ggtttcgata tgccgctgct cgtggtggtg gttgctttga tcgtgttcgg actggcgatg
     6421 ttgtattcct cttcatggga tttctcgctc ggtgcatacg gcgatgcaat gtacatgttc
     6481 aaccgccagt tgacctggct tgggcttggg ttgttctcgg cgctggtgct ggctttcttt
     6541 gattatcaca actggcgcaa gtatgttgtg cctgccatgg ccctgaccat cggtctgctg
     6601 gtcgttgtgc tgttgatgaa tgaaattcgc ttcggcgcca aacgcgcgat ccttggcggt
     6661 tccattcaac cttccgagct ggcaaagctg gtttcgattt tatatctcag cgtttggttg
     6721 tatgccaagc gccaatacct gcacgatatt tccctcggct tgatcccgct gggcgtgatc
     6781 ctcggtgtgg tgggtgggtt gatctaccaa caacccgacc tgagcgctgc cgccactgtg
     6841 cttatgctgg gcgggctgct cttcttcctt gcgggcggcg acctgaagca gatcggtgtg
     6901 ctgcttgtcg ttgcaaccgt tgccgcatgg atcgttgctt ccatcagcgc cacgggtcag
     6961 cagcgtgtgg gtgattttat tgcaggcatc aaagatcctt tgcaggcttc gtaccatgtg
     7021 cgccgctctt ttgaagccat tgtcaatggt gggtggtttg gtgtgggcct cggccaatcg
     7081 caatccaaac ttaccgggct gcctgtgcct cccaccgata gtgtcttcgc cgtggtggtg
     7141 gaggaacttg gctggttcgg tgcagtgggg ttgatcgttc tgtacggtgc gctggtctgg
     7201 cggggaatgg tcattgctcg tcgtgcgccc gacatgcttg gcacactgct tgccaccggg
     7261 ttggtcacat ggatcgccat cgaagccctc gtcaacatgg cggtgatggt gggcctgctg
     7321 cctttcgcag gcaatgccct gccttttatc agcgcaggtg gttcaaatct cgtcgccaca
     7381 atggcgtcgc ttggaattct ttttaacatc tctcgccaga caggcgaagg cacgatctca
     7441 gatgacgaat ggaggtctta cagtgcggtt gttaatttgc gctggtggaa cgggcggcgg
     7501 ggtttatccc gcactcgccg tacacaacgc gctaaccagt cagcgccaga aagttgaaac
     7561 actctgggtc ggtggcgaag gcggcatgga agaggaactc gtgaatcgtg cgggcatccc
     7621 tttccgttcc atccctgccg cgggtgtgca tggcgtgggc ctgcgcgcac tgccgggcaa
     7681 ccttgccaaa ctcacacgcg gtgtgcaagc ctcgcgccgc atcttgcgtg aattcaaacc
     7741 cgatgtactc ttcttcaccg gcggctttgt cgctgccccc atggccgttg ctggaagaaa
     7801 catcccaacc gcgttgtatg ttcccgatat cgaacccggg cttgcgctta aattcctttc
     7861 ccggtttgcg gatgtcgtca ctgtaacagc gcctgactca aaaaaatatt tcaaccgccc
     7921 ggaaagactc gtcctcaccg gctacccgct tcgctctgac ctttcaaact ggtcccgcga
     7981 aaaagcaacc cagcactttg ggttggatcc acaaatgccc accctgcttg tcaccggcgg
     8041 aagcaaaggt gcgcgttcga tcaatatggc tgtattgaaa cacctccatg aattgctcgg
     8101 cgcagcacag gtcatccaca tcacaggctc gttggattgg cccgtcattg agaaagccgc
     8161 acagacattg cctgcagatt tacagtcacg gtatcatgcc atgccctact cacatgaaat
     8221 gggcgccgct ctcgccgccg cagacctggt cctctcacgc gcaggcgctt cttccctggg
     8281 agaatatccc ttctttggtc tgcccgccgt gcttgtgccg tatccatatg catggcgtta
     8341 tcagaaagtg aatgccgatt tcctcgttga aaagaatgcg gcagtccttt tgcaggatga
     8401 actcctggaa gagaacctgc catctgttgt gaaaggttta ctgttgaaca aaaccaaact
     8461 cgaatccatg cgtgccgcca tgcaggcttt atcacacccc gatgcggcag gcagtattgc
     8521 cagccagttg gttaaattgg caggagcata aaccttatga tgagcgttgt ttatatcttc
     8581 tggatgtata tcattctctt tggcattgtc ggcgccatgc gcggatgggc caaggagttg
     8641 atggtcatct tcagcgtaat tactgcactg gctgtgaatt tgctgttgga aaaatacatc
     8701 ccccttgtcc gcgatctcga taaggcatcc agttccatgt tttggatccg cagccttatt
     8761 ttgatcacac tggtgttttt tgggtatcaa acggtcaacc tcacgcgttt ttcaggcaaa
     8821 gccgcgcgcg aaaaactgca ggatgggatg ttcggcgccg tgctgggcgg tttcaatggc
     8881 tatctggtgg cagggtcaat tcttttctat aaccacatgg ccctctaccc gtattcgaac
     8941 attatcagcc cggccacaga cccggccatc gctcaggcaa ttgaaacgat gatgaaatat
     9001 atgcccccgc gctttttggg cgaaccgagt atatacttcg ctgtcatcat cgttttggtg
     9061 ttcattattg tggtgtatat ctagcgattg gctgtgagcg gttggcttgc ggctgcattc
     9121 caatcgccat cggtaaatgg ctaggagcta aattatgaaa cgtgttcatt tcatcggcat
     9181 cggaggttca ggactttccg ccatcgcgcg cctgctcaag gagagcggct acgaggtgac
     9241 gggttctgac cgcacgcttt caccgttcgc agtggatttg cagaataacg gtgtcagcat
     9301 ttacgttggg catcacccgc gtaatgtttc aggtgccgat attgtgatcc gttcctccgc
     9361 cattccagat gaaaacgttg aagtggaagc cgccaaacgt gcaggagtcc ctgtttacaa
     9421 gcgctctgat tttctcgggc agttgatgtc tgaaaagacc ggtattgcag tggcaggcac
     9481 tcacggcaag accaccacca ccgccatgat cgcatggacc ttgactgcct tgaagcgtga
     9541 cccatctttt attgtgggtg gcacgttgaa caacctgaaa gtcaacgccc gtgctggcaa
     9601 gggcagtccc tttgtgatcg aggcagatga gtatgaccgt atgttcctcg gtctcaagcc
     9661 gcagatcgcc gtggtcacca accttgaaca tgaccacccc gattgttacc cgacctttgc
     9721 cgacatgtat tctgcttttc aaggcttcgt tgacctgctt cctgaagacg gcacattaat
     9781 tgtctgtgcg gaagatgaag gcgcggtcag tttgctttcc tatgcacgta aaaaaggcct
     9841 gagtgtgatc tcttacagcg tacaaagcga gttgaccatc aacagtccgc aatggatgca
     9901 ggcacgcact cccaagccca atacccgtgg cggttttgat ttctccgcaa tgacaaacat
     9961 gggcaccgaa tcggcttcgg tggtgaatgt ttcactgcag gtgccgggtg aacataatgt
    10021 ccgcaatgcg cttgcggctt tgtctgtcat ggctacgctg ggtctgcctc ttcaagaagc
    10081 cgcagacgct cttggtgaat tcaccggcac gggacgccgt ttcgagattc gcggtgagca
    10141 gaagggtgtg attgtgatcg atgattatgc ccatcatccc actgagatcc gtgccactct
    10201 ggcgggtgca aaggctcgtt atcctgaccg ccgcatctgg gcggtttggc agccgcatac
    10261 ttattcgcgc acacagactc tgttctttga attttcccgc gccttcagcg atgcggatga
    10321 agtgctggta accgaggtgt atgcctcacg cgaggccaaa caggaatttt cctcggcgga
    10381 ggtggttggg tccatgccgc atgcatcagc acgttacagt ggttcactgg aagatacgac
    10441 cgcatacctg ctgaagaacc tgcgttccgg tgatgtgctt ctggtccttt cggcaggaga
    10501 tgccgaccag atcagtacga atgtgttgaa gaatttgtga gatgaccatg ttggagaatc
    10561 acgaaaagca aatggctcat ctttatgaag tgatcgcgct cccgcctttg aaaatggcgc
    10621 tgttccacgc ggtggatgag caattgacaa aggccgccct gatcaacgcg acccgcaaac
    10681 tctctgcaat tccggtgttg ggcagtgaga atgtcgggaa gcaatctttg gaaaagatca
    10741 tcggacactt ggaaaagaag aaactgtaaa aatggatatg gcccgttctg cccttcatgt
    10801 tgatgtgttg cgccagatct ttggcgatgc cgtgcaggag aatatctctc ttgcgccgta
    10861 cacatctgcg cgtattggcg gcccggcgga tgtgctgttg acggtcaaat cggcggacga
    10921 gtttgccgag gcaatgaagg tcatttggga aaatgatttt ccatattaca tccttggcgg
    10981 cgggtcgaat gtgctggtca gtgatttggg gctgcgtggg gtggtggttt tgaatcgggc
    11041 gaaggaagtg cgcttcgaag gcggcgacca gcccacggtc tggtgtgaag cgggtgtcat
    11101 tttcagcaac ctcgccaatc gctgtgcatc caaaggtttg gcaggtttgg aatgggcggc
    11161 gacagtcccc ggcacagtag gcggtgcagt gtatggcaat gctggcgcgt tcggcggcga
    11221 tgtgtcgatg aatttgatct gggccgatgt gttgacaaaa aacggacgtg aacgaatgtc
    11281 tgttgaacaa atgggatatg gatatcgcac cagcgtattg aagcgcggtg aaattcatgc
    11341 gatcgtactt tcagctttgc tgagattaaa aaattcagcg aaagaggaag tgtctgttaa
    11401 aatcgagcaa ttcagcgaac gccgcaaagc cacgcagccg cctggtgcga gcatgggttc
    11461 catgtttaag aaccctgaag gtgatcatgc aggtcgtcta attgaagctg ctggcttgaa
    11521 aggtgtacgt atcggcaacg ctgagatcag caccttgcat ggaaattttt tcattaatca
    11581 cggcgagacc cgggcgaagg atattctcgc cttgattcgt ttgacgcaaa aaactgtgcg
    11641 cgaaaaattc ggcgtgcagt tggagttgga agttgagctt gttggagatt ggaacgaata
    11701 accgcgaaag cgtttgttct tgaaacgata tgaagaaact tcgagtggca gtgttatttg
    11761 gtggtcgttc cggtgaacat gatgtttcac tcatgtctgc gcgctccgtg ctcaatgctc
    11821 tggatgcgga acgctacgaa gtgatccaga tcggcatcac gttggacggt gattggttca
    11881 ccgggccaaa tgcgttggat gcatttgaaa atggaagcgt ggatgggctg gaccgtgttg
    11941 taccgccgtg ggagccatcc tatcaatctt tgtatgtcat ccgttcgaca aagcttggcg
    12001 agaaacttga aacactctcc ggggtggatg tattcttccc cgttttgcat gggccgtttg
    12061 gtgaggacgg cacaattcaa gggctgcttg aactggcaga tgtggcctat gtgggggcgg
    12121 gggtggctgg ctcatcggtc ggcatggaca agggtatttt caaggatgtg atgcgcgcaa
    12181 acggaatccc cattgttgat tccatcctca ttctgcgcga cgagcttgaa aaggatatga
    12241 acgcggtcat cgccaaagtt gagcagatgg gcgcttatcc gttctttgca aaacccgcca
    12301 acctcgggtc ttcggttgga attagcaaat gcaattcacg ttccgacctg ggcgaaggtt
    12361 tgatggaagc cgcacgatat gaccgccgca tcattgttga acgcggagtt gggaatgtgc
    12421 gtgaaattga agtcagcgtt ttgggcaatg aatacccgca gacctccgta tgcggagaag
    12481 tgcagcccag ccgcgagttc tactcctatg aatccaaata tattgacggc acctctggcc
    12541 tcatcatccc tgcgattttg ccagccgatg tgagtgacca gatccgtgat tatgccgccc
    12601 gtgcctacaa ggctattgat tgtgctggca tggcacgtgc cgatttcttt tttgataacg
    12661 atatgaaaaa gatctacctg aatgaattga acaccctgcc gggctttacc tccatcagca
    12721 tgtatccgaa gttgtggcag gcaagcggtt tgaactatcc gcagcttgtg gaccgcctga
    12781 tcgaacttgc cttcgagcgc aaggcccagc gtgatcacac cgagcgacgg aggacagcat
    12841 gagcgaacgt caacgcccca cccgtgctga acttgcccgt cagcgccgaa atcaacgcgc
    12901 cagaaaagaa ctggagttga ccaccaagcg cgccctgaag ccgatgcaat cagtgacctc
    12961 tcgtgtgcct gagattgtgg ttgctcccaa attgactcgt cagtccgcgc cgcgccgccg
    13021 ctttaacatt gcactgggtc tgccggaaat tcacctgcac aaaccccagt tccacatgcc
    13081 gcgcattcgc cccagttggc ggctgacatc ttttgttctc gttgttttgc tgggtgtggt
    13141 catttacctt tcgttgaccc tgccgtattt ccacgtgcca aacatcaccc tgttgggtgg
    13201 gggtcgcctg agccgcgaag agatcgaagc agtgcttgct gtgaacggcc agtccatttt
    13261 cacagttcaa cctgatgaat tggaagttcg tctgcgcatg aattacccgg aactttcgtc
    13321 ggtggatatc catgcttatt tgccgaacca tgtttacgtt acagtagtgg agcgcgagcc
    13381 tgtcatcctt tggcagcagg gcggtggatt tacatggatc gatgcgtctg gcgttgcttt
    13441 tcgtccgcgc ggcatggttg cgactctcgt tccggtgaac ggtctttcca cgccgccagc
    13501 tggtcagcct gccacggaag atccgctcag cccgatgccc tacgtttcga aagaactcgt
    13561 ggattccatc ctcatccttg cccccaatgt gcctgccggt gcaacgatgt tttatgatgc
    13621 cgccaacggc cttggctggg aggatgaacg cggttggaag gcctattttg gaatcagtgc
    13681 ccatgacatg ccattgaaga ttcgggtgta tcaatccctg gttgcatctt tgacaagccg
    13741 tggcaccgtt cccgtattta tcagcgttgt gtatcctgat gcgccgtttt atcgcatggc
    13801 tgatctcgaa ctggatgcca ccgatgcagc atcggaagat gcaattattg aagatggaca
    13861 acagtaaaga tggatgaaat cgtagttggc gtggatgttg gcacaaccaa aatttgcacc
    13921 cttgtcgggc gggtggagga tgcaaagacc attcggattc ttggcgtagg gatcgaaccc
    13981 tcggacggaa tccgcaaagg gatcattgtt gacctcagtg ccgcatcgca agccattaaa
    14041 cgttcggttg aaaaagctga gaacacttcc ggtttggaga tcaccactgc cctggtcagc
    14101 ctggcaggtg cgcatgtttc ttccgtgaac agccgcggcg cttctggaat ccccggtggc
    14161 attatcgatg caatggatat tgcccgtgcg ctcgagcagg ctcaagcggt ggccattccg
    14221 catgaccgcg aaatcgttca tgtcattcag cgcggcatca cagtggatgg gcaggagggg
    14281 atccgcaccc cgattgggat gcatggttac aagctggaag ttgaaacgca catcatcacc
    14341 gcagcggcag ccacggtgga taaccttcgt caatgtgtcg gcgcggctgg tgttgagatt
    14401 cagcaatttg ttttaaatcc gctggcatct gctgaagtgg tgttgacgga acaggaacgc
    14461 cagatgggcg ttgcggtttg tgatatcggc ggcggtacaa ccgacttggc catttatgtg
    14521 aatggcgatg tctggcatac catggtgttg gccgtcggtg gaaatcatgt gacacaggat
    14581 attgcacatg gtttgcgcct gcccatggcc caggccgaag aagtgaaaaa gcaacaggga
    14641 tatgccatcc gctctgatat tgccagcgat gaatatttct ccattcgccc ctttggcgag
    14701 gataacgatg tgaagatcaa ccgtcaggat ctcgctcata tcatcgaagc ccgcatcgaa
    14761 gagaccttcc gcctgattct gcaggaagtg aaacgctccg gctacgatgg tttgctcccc
    14821 gctggcatgg tgttgactgg tggtacctct gcattgccag gtatcaagca aattgccagt
    14881 gaagtgctgg gcatgccggt gcgtacggct cagccggaaa accttgttgg tctggttgat
    14941 aaacttggtt cgcctgcata ctctacaagc gttggcttgc tgcgttgggc cacaaccatg
    15001 cgcgatcatg atgtgatcgt tacaagcggc aacaagaacc gccgcagatc gagaggagaa
    15061 agttcaatga atttggattc gatcaagaat tggatgaagc ggctgttacc ctagttttgt
    15121 taactttctt ccaaaaacat ggaaagaaag tttaatgaga agattcacaa agattgttta
    15181 aaccctttta aaaatcgtca ttctcgttta agattggcat atccttgcca ggaggaacac
    15241 atggacacga acaaaagaaa tccaaatacc gaagccttcg cccgcatcaa agtgatcggc
    15301 gttggtggtg ctggtcagaa tgcagtcaac cgcatgatgg aagagggaat tcagggcgtt
    15361 gaattcatct ctgccaatac cgatgcgcag gcgttgacgc tttcacgcgc gcccatccgt
    15421 gtgcgcctcg gcgacaaact cacacgcggg cttggcgcag gcggcgatcc tgagatcggc
    15481 cgcaaagccg ctgaagaatc ctctgatgaa ctctacaatg ttctcaaagg cgcagacatg
    15541 gtcttcgtca ccgcaggtat gggtggtggt acgggtacag gtgctgctcc cgttgttgcg
    15601 caggtcgcca aggaatgtgg cgcattgacc atcggtgttg tcacccgtcc tttcaccttt
    15661 gaaggcggca agcgcacaca gtctgctgaa tctggcgttg cgaagatgaa ggaacacgcc
    15721 cacaccctca tctcgatccc caatgaccgc ctgcttcaac tggccgacaa gaaatcatcc
    15781 ctgcaggatg ccttccgcat ggccgatgaa gttctgcatc agggtattca aggcatttcc
    15841 gaactcatca cgatccctgg tttgatcaac ctcgattttg ccgatgtcaa agccattatg
    15901 tctgaaggcg gcgctgccct tatggctgtt ggtcgcggtt ctggtgatga acgcgccaag
    15961 atcgctgcgg agcaggccat ttccagtcaa ttgctcgata tcaccattga cggcgcacgc
    16021 ggcgtcctct tcaatgtgac cggcggctcg aacatgaccc tcttcgaggt caatcaggct
    16081 gcagccatta tccgcgaaac cgcacacccc gatgtcaata tgatcttcgg tgcggtcatt
    16141 gatcctgaaa tgggtgacga gatccgctgt acggtcatcg cgaccggatt tgaacgcacc
    16201 agcgttccgc gccgcgcttt ggaacgccct gctgcccgca ctgagaaacc gaccgcggct
    16261 tcagccagtg cattgttcgc ccgctcaagc gaatcggtca gcgtcagcgc cgattacaag
    16321 acccctggcg aatccaagcc tgcgtctcag ccctccatca gcaacgatga tctggatatc
    16381 ccgaccttct tgagaaatcg aagataaacg atgaaagcaa agccctaaag gttttcaaaa
    16441 cctttagggc ttttttgttt tagggaacaa acatgctgaa gtatcacatt acaaatcgtc
    16501 gcgtgttacc ccttcaaact gtaaaatgaa atctgagccg taggctttgc ttggggtctg
    16561 aaagccaggt ttgaaatctt cagaagtgat gcgtgtcatg atttccacgg ctgttaatgc
    16621 ggtcaaatag taggcttcgg gggtgcgcag ctttgcacgg actgtctgtt ttccattcgt
    16681 tgcttcggcg atcatcaagc tgaagccatt ctcgttttgc tggcgtgatg gacctgtttt
    16741 gagaaaaacc ccgatcagcc atttgatgaa atcttttgca gcgcggttgt aaagcaaaaa
    16801 tccaagatgg cgggtgaggc gcatcccatt gaccattgca gcggggaagc ccatgtatgc
    16861 tgtcacattt ggaatgcctg tgctgtgata agccgtactt acatcccccc agccaactga
    16921 aaccacgcgt gaaggaccgc gcccaaagtc cacctgcttg ctatcccaaa cattcggcac
    16981 agacacgatc tctccattgc ggcggatgcg tccctgccgc tgcatattct cgatacccga
    17041 gcgggccgta ccgcgtgaga ccccgctgcc aatgcttttg atgtaaatct caaggtgggt
    17101 tgggtttgca agtttttttg caacatgtgc aatcaaacaa tctgaaggaa ccacatcgaa
    17161 gccgccgcca ggcagcagca tgatgccaac tcgttttgcc tcttcatcca ttgcagccaa
    17221 tgcttcaaac ccttcaatct cgccgctgat atccacataa tgtcgcctgg tgcggataca
    17281 tgcctccacc atctggcggt atgtcaagac gaacggcccc gcgcagtgaa gtacggcatt
    17341 tacttccaat aaagctgcgt cgagcgcagc agaatgatcc attgaaaatg cgcgatattc
    17401 caacccgaac ttttcagcct gtgtgcgcaa tagattttca tcgcgccccg caagtaatgg
    17461 cttcatcccc gctttgactg cctgttcaac gatcaaactg cctgtatacc cgtaagaccc
    17521 atagattagg aaatttttca tgatctttcc gtccatgacg ggttggcata tttttcctgg
    17581 attaattcct ctgctctttc agattcggat gcagacaatt ctccacgctg aaagtggatt
    17641 cccaactgcg attcaaatcc tttaacaaag gcctgggctg ctgtttccca atcaggattc
    17701 acccccaaag tggattcaac ggtcgttgcc cgtgccagca aacgttgggc tgcacttgcc
    17761 cgcgcagttt catccctgaa gaccagagcc tgacaaatgc gcgtcaggtc tcccgtaagt
    17821 gggagcgagc catgttgaag tacgccttcc tttttgcggg actgcgctga gccgatgagt
    17881 ttttttcctt ccacagtgat ctcatacgtg gatggcactt caaagcagac ggggttaagg
    17941 tttaaggttg caggttcaga gatatgttcc ttgatttcaa caggcagccc caactcacga
    18001 atggcgaata acaaagcctg cgcgagacgg ttatacgatt ccagaacgcc gcctgccagc
    18061 acgggttcat ctgcaccgcc tgtgaccgag taggtcaact catcggtgtg taaaatggca
    18121 cgcccccccg tcacacggcg gacaacttcc caaccgtttg cccgcaatct ttccacatcc
    18181 acatctgcaa aggattgtgc gtggcccaaa gacaggcagg gtggattcca tgaatataac
    18241 cgcagggtag gcagcgattc tccgcggtga atgtgttcaa ggatggattc gtccacggcc
    18301 atattccatg caccgctgga tgggggagtg taaaggagac gccagttcat tttggtttaa
    18361 aggttggaaa gttcgaaagt tggagagggt gaacctgtaa acatttaaac ctgcaaactt
    18421 tcccaccctt gcaggatttg atcttcaagt tcaggtgaaa tatcttccaa cggcgggcgg
    18481 accgtccacc gtggcaggga gtgtagtttg tgcagtaatg ctttcaaagt gggcggaaac
    18541 ggagtgtact tttcgagggc gtgtctttgt tttgcaaccc gcacctgaat ttcagatgga
    18601 tccccacctt cattgtacag gtcccagata tcgcgcagat cgctgccaat gaggtttgcc
    18661 attgcggtga tggccccttg ggcatgattc ttcaatgcat ggtggaagtg cgaatccgtg
    18721 ccattgagta cgagcaagtc tctgccgaag cgttgaccca ctgccgccga aaaagcctca
    18781 tcatgcgagg agtctttaat gcccgcaaat tgatttggga acgcatcttt caaacgggcg
    18841 atcagatcaa gtgagaaacc aatgcccgcg gcgctgggga tgtgataacc aagtagatat
    18901 ttatgggctg gcacggcttt ggtgatcaac tcactgaacc atttgaacag accttcgtct
    18961 gctgctttgc ggaaataata tggcggcaac accaccacac cgtcataacc gagatcgaag
    19021 gtcagtttgg tcaattcgat cgtttcggaa aggcttggtg ttcctgtgcc tgccagcaat
    19081 ttgaacccag gcagctgttg gcggtacaca cgaacggaac gcagcagaac ctcacgctcc
    19141 tttggagaga aggacgggcc ttcacctgtg gtgccgaaga gtagtgcgcc gtggcatccg
    19201 ctggcggcga gatgacgcaa aagagcagga acagcttcaa ggtcgaagtt tgaatcgagc
    19261 ttgcttcctt tggcagggct atcgagtagg ggagtcactg ctgcagcgta gactcctgca
    19321 agtggatgta gatcggtcat ggttatttct ttttcttctt tctgatgaat ctttgtgcgg
    19381 cttcatgtgg cggttgtaac ggctggcctt tttccttcga taaataatgt tgatgatcca
    19441 gggtccacaa atacagatcg ccttcggttt tatcggggaa gttcttcagg atgtcgcttt
    19501 ttcgcaccac gtcaatgatg ggcatgtaga ctgtatcgta ccagtgtgcc acggcttcct
    19561 cctccgtgac atcccgtttc aagtccagcc ccataaaata gtgatgaacg gcgatgtgtt
    19621 cgagcatgcg ttcaaagcca tcgggaatgg ttagtttgat gttcgcttca gggcggatat
    19681 ggtcaagcga ggaccgttcc agaaaatgca ctttggtgcc gagaatttca aggtcttcgg
    19741 gctggatgtt tggtgtgagg tttacacggg tggcacattc acggatctcg gcttcaatga
    19801 attcctgtcc ctgctcacgc gccactgaaa cgcggtgatg accatcaacg acgaaataca
    19861 cttcgcccac tttgtacaac acgacaggcg ggagattcac atcctgataa aaggcacggt
    19921 cgactttttg ccagcggctg gcaagctggt cttcacgcgg aagaaaagcg cggtcaaatt
    19981 cgtgatagcg attcaaacta cccgcgatct gctccacgcg aatggttttg actccccgat
    20041 aaatggggcc gccgatatgc agcttttcct tgacatcatc gtaggaaagc agggtggtgg
    20101 ggcgctggcc ttttagaacg gcaatcgcct ggttgataaa ggatttgaag cgcgctctgc
    20161 tgaaatcagc acgcactctt gaggttaatt cgtcagacat gactagtggc tcacttctat
    20221 ggttttatat ggataaacat taatgacttt ggtcaggccg atgttggttt ctacgttctc
    20281 caggttgcgg cggtacaggt gggtgtggcc gtgaaaatgg tatcgcggct ttgtgatgcg
    20341 gagcagccag ttgagggttt tcaggccctg atgggcgggg tctgagtcat cgtggatgcc
    20401 aaacgggggc gaatggctga taaggatatc cagcgggcgg ccatattgga tgcggtatgc
    20461 cagcaggcgg gggagcatgc tgaaggctcg atagtaggcc tcttgctggc tgtattggtt
    20521 gacgccatcc gggcggtatc gaatgcttcc gccgaagccg ccgatcagga attttttgtg
    20581 gcgcaccgtt ttcaggtcaa gattgacgcc gccttccggt cgcacagatt cctgtcgtgg
    20641 gttgtgttgc gggtcgtgat tccccggtac gtaaaaaagg ggaacattta gcattgtgac
    20701 cagatattca aggtattcga atggcatatc tccgcagcca aggatcagtt caacatcctg
    20761 aaaatggccg ctggctgaaa gtgtgtagag ccgttcgatg gcctggtcgc tcactgcgag
    20821 aatcttcata gcctttccat tttgccgtag aacgtaaatc ctcgcaagcc agtttaccga
    20881 cgaaattgca atttccagca tttgcaattc cattaactgc acactataat cgaagaacca
    20941 gtacgaaacg ctcaggagtc ctctctggaa accagaaaca tcacttcccc aatagggtca
    21001 gattcaggcg atagccattc cgcctcccgc cagcaaatac aatctggtat tacactcgtg
    21061 gatcgctaca acatccagga tgtgatcggc gtgggaggca tggggtcggt ttatcgtgcc
    21121 cgcgatctgc actttccaaa cgtggtcaaa ttggtggccg tgaaggaaat gatcaacagc
    21181 gcgccagacc cgcttgtgcg tcagacgatc gtgcaaaact ttgagcgtga ggcaaatttg
    21241 cttgccacgc tcaatcaccc tgccattccc cgtatttatg attatttttc actggatgac
    21301 cgctcttacc ttgtgctgga gttcatccac ggcaaggacc ttgaagcggt catcggtgat
    21361 tcgaacggtt tcttacctga agaccagatc atcagctggg ccattcaact ctgtgacgtg
    21421 ctggcgtaca ttcatggaca caaaccagac cccatcatct tccgtgatat gaagccttcc
    21481 aatgtgatga tcaatcacaa tggagatgtg gtgttggtgg attttggcat cgcgaaaacg
    21541 tttcagtccg ggcaaaaagg caccatgatc ggtacggaag gctactctcc accggaacag
    21601 tatcgtggtg aagccacccc attggctgat atttattccc tcggcgcgac cctgcatcat
    21661 gccatcaccc gtcgtgatcc gcgccttgaa ccgccttttt catttgccga gcgtcccatc
    21721 cgccgcataa accccaatgt ttcgctggag tttgaagcgg tggtaaatac ggctttgcaa
    21781 tacaaccctg gtgatcgttt ttccagcact gcagcgatga aggatgccct tctaaacgtt
    21841 gcgcgcaaga cgggtactct cgaaaaagtg gcagctgctt tgccggtatc cagcggtggt
    21901 gtcaaaccgc tgtggacctt caaatgtgaa gatgagattc gcggcacccc ggccctgcat
    21961 cagggcatgt tgttcatcgg gtgctatgat aacaacctgt acgccttgaa tgcggctgat
    22021 ggtcagtttc agtggaagta cgccactgaa ggcggcattg tttcgcgccc aacagtattt
    22081 gacaataatg tcatttttgg ctcggaagat catcgcctgc atgttgtcag tattcgcagc
    22141 ggcaaggtgg tgtggacgta ttacaccgaa ggagagatct actcttcgcc gcgtatcgcc
    22201 gatggccata ttttcttcgg ctccgatgac caggacctgc atgcggtcaa tatcaccagt
    22261 ggccgcgcca aatggaaatt tgccacagac tctcccattc attccacgcc gttggttgca
    22321 aatgaaatga tattttttgg caacgagtct ggcggatttt attccgtcga tttccgcggt
    22381 gagcagaaat ggcgttttca atccaaacga gccgtcacct cttcgccggt tatcaagggg
    22441 caggcgattt attttgcatc tgtagacggc actttgtacg cgctggattc gaaaaacggc
    22501 tggccgatct ggaagttccg catggggaag ggttccattt cctccccggc cattgcagat
    22561 gactttattt tcgtcggcgc tgcagatgga tttctctatt gcgttgatac gcggaatgcc
    22621 aaggaagtct ggcgtttccg tacggaaaat caggtcagcg gttctcccgt aatttacaag
    22681 gattcgcttt attgcggctc tgttgatggc agtttgtact gcctggaata tcgtaccggg
    22741 cgattgcgtt ggaagtttga aacagccgcc gccattaccg gttcgcccat tgtgttcgat
    22801 gatattgttt attttggttc tacagaccat caagtttatg cattgttcgc ttaaaaggag
    22861 tctctcgtgg ctgatttttt ccgaaaacta tttggtacaa aaccgactga ggaacctgcc
    22921 aaaccaaagc aggttgatat ggccacaact gcgccgcttt ccgaccagca gatcaactcc
    22981 attattgcga atcaatatgt gaggtatgaa acgaaacagg tcattgccag tgtgggccag
    23041 tcggttggaa aacagcgtga acataacgaa gatagtctgt tggccatcac atccaccatt
    23101 gccggtagca cagaaagcat cccttttggg ttgtatatcg ttgccgatgg catgggtggg
    23161 caccagtttg gtgaagtagc cagcaatgct gccgtacgca tcatgggcgg caacatcact
    23221 aaaaaattcc attcctacct ttacaatttg cctactcagg ctcttcagga atccctgcaa
    23281 gaggtgatgg aatcgtctgt gatggaagcc catcaatacg ttcagcgtga agcgccgggc
    23341 agcggcacca cagttactgc tgcattggtc ctcggtcaac aggtgaccat tgctcatgtg
    23401 ggtgatagtc gtgcctattc catttacccc gatggaagga tcgaaccgat cactcgtgac
    23461 cattcactgg tcaagcgact ggaagaactc ggacacctca gcaaagatga agccgctaat
    23521 ttcccgcatc gcaacgtttt gatccgtgcg ctcgggcagg gcgaatcact cgaagccgat
    23581 gtattcaccg tccccttccc gcaggcaggt tatttgatgc tgtgcagcga tggtttgtgg
    23641 ggtgtcatca gtgaaaaaga tatctatcgc tcgattgcag aagctcccaa tctccaccgc
    23701 gcctgccaaa acatggtgga agcggcaaat ctggcgggcg gccccgataa tattacagtg
    23761 atcctcgttc aaatggttgg ataatcatgg cgtccagcaa atcggaccat tacgccactc
    23821 tgggtgtttt tcgcgatgcc tcacctgagg aaatcaaacg tgcatatttt gaagctgcac
    23881 agcgattgca cccggataag aacgtagcgg ccggtgagac cgaacttttt cttgaaattc
    23941 agcaggcata cgaagttctc tccaacccca aaagaaaaaa tttatacgac gccacactcc
    24001 ccccggagat aaacgtcaac accatcgtaa aacacgatgt gacatttagc cgcccaaacc
    24061 ttgtcaaaat aggtgagccg cagctgcttt atgccttgct ggagatcagt ccgcgcgagg
    24121 ataaggaaag cctgcccgct ccaccgctga acatctgcct tgtgcttgac cgctccacat
    24181 ccatgcaagg cgacaaaatg gatatggtca aggcaacagc cgttcagttg ctgcgcagcc
    24241 tgcgccctga agatgtcttt agtgtggtcg ctttcagtga ccgtgccgaa gtgatcgtcc
    24301 ctgctgcgtt taatgttgac aagaagaaac aggaagcccg cattcaaatg atgcagccat
    24361 cgggcgcaac tgagatcttc aacggcctga aggctggttt gcaggaagta cgccgcacca
    24421 ttgatccctc ccgtgtcaac cacattatcc ttttgactga tgggcaaacc tacggtgacg
    24481 agcaggaatg cctgaaactg gctgaggaag ccgcagccca gaacattggc atcacaggca
    24541 tgggcattgg tcacgaatgg aacgatattt tccttgatgc ccttgccagt aaaacaggcg
    24601 gttcatctgc atatatctcg aagcccaagg atattcagca gttactggtt gataaattca
    24661 atgccctcat cagcacatat gctgatgaag taattttaga tttcaaacca cgtgacgata
    24721 tcaaaatcaa atacgcattt cggttacagc ctgaaggtgg ttcaattgcg attgaaaccc
    24781 ccatgcggct gggtccgatc ctgcgggatg ccccgctcca tgtgttgatc gagtttattg
    24841 tggagccttc ggcgctggat gaagaagttg tcactgtctt aaatggtatg ttgaaggtgt
    24901 ccattaccgc ccgtgcggcc ccggtgccgc ccattcgtat caacctcaag cgagatgttc
    24961 actccaaccc ttcagccgag ccgcctccca ctcgtatctt gagcgcgctt tcgcgcctga
    25021 ccatgtaccg aatgcaggaa cgggcacgta ccgctgctga tgcaggtcaa tatgatgaag
    25081 cagcccgtca tttgcataat ctggcaactc acctcctctc aaaaggtgaa aactctcttg
    25141 ccaaaaccgc cttgtttgaa gccgaaaacc ttgaaaaaat gcatgcgtgg agtgccagtg
    25201 gtaccaagga tattaaatac cacacccgtg cattgctcat gggcggttca aaggagaaag
    25261 taaaatgatc atttgttcaa attgtaaaaa tacaaacctt gatggggcga tgttttgtgc
    25321 cgaatgcggg tcgcagttgg ttggcaaaga tactctcatt acacagaaca tctcaacaaa
    25381 gcaattcgaa aaagcccgcg aaagcaaacc cgccagaccc aacgatacct atcaaccttt
    25441 cgatggcagt gatgcctggg gcagcctgca cctgcttgat accgggcagg tcctgccgct
    25501 ttccaataag aacgaattca ccatggggcg tatcagcgaa gggcagccca tcatgcctga
    25561 tattgacctt tctccctatc aggcgtatgc cgcaggtgta tcccgtcttc atgcagtgat
    25621 taagcgcgag gcagggaccg gtcgcatcat tttcattgac ctcggttccg ccaatggtac
    25681 ttatatcaat gggaagcgcc tcaccccgaa cgttgaacag gttcttgtac atggcgatat
    25741 tgtggccctt ggaaagttga aattacaaat acttgtaaaa agcggataga gtaggaaagt
    25801 gagaagatca tgtcttatac agtcatcctt catatttcag gtgaaacctc ggtggcggga
    25861 gagatcgaag aactcccaaa acccaccgac accatcatta tggtaatgaa tccccgccag
    25921 aaagatggca aagacctgca ctatattgat aacaatgtca caaaagtcat ctggccgctt
    25981 gccaaactga gttttatcga gattctcgaa ggcaccgggg aagagaagat catcagcttc
    26041 gtgagggaat aaggaaacca tgtctgattc attggaacgc cgtcgtattc ttgttgtgga
    26101 tgatgaagag cgcatggtgc gcttcatccg catgaacctt gagcatgatg gttttcaggt
    26161 cagtgaagcc tttaacggca aacaggccat tcaaaaactg cgcgatgtaa cgccagatct
    26221 catcctgctc gatgtgatga tgcccgatct ggatggtttt gaagtgcttg aaaccatccg
    26281 cgagatcacc actgtgcccg ttatcatgct cacagccaaa ggggaggagg atgaccgtgt
    26341 gcgcggtctc gaaggcggtg ctgatgatta tgtcaccaag ccattcagcc cgcgtgaatt
    26401 ggtcagccgt gtcaaagccg tgctgcgccg caccgaaggt gcaacgggtt ccatgcatgg
    26461 tctgctcgaa attgacaaac gcctcaaaat tgactttgac cgccgcgaaa tctggctcga
    26521 aggcaagcta gtcaaactgc gccccaccga ataccgcctg ctttttcacc tcgttcaaaa
    26581 tgcgggttgg gtcgtttcac acgatcaact gctccagaaa gtttggggct atgaataccg
    26641 cgatgagccg cattacgtcc gcctgtatat taactatctg cgccagaaac ttgagaaaga
    26701 ccccgccgaa ccaaagtaca tcctcaccga gcgaggagtg ggataccgct ttgtagatta
    26761 caagcgcagc aaagagtaaa ccatgtccga tcagaagcct gataataagc tggtcactgc
    26821 cgccaaaatg atcgccattt gcgtcacctg ttttggcact atttatggcc tcgtttcact
    26881 ggtcacattt ctaaccgcat aagatttcta acaaaatgaa tacaaaacgc ctgcgttttt
    26941 ttaacgcagg cgttttaaat taagggcgta agttcaaacc aaacaaccct aaaaggaggt
    27001 acctatgtcc aatttaattc gttgggaacc cgctcgtgaa atgatgactc tgcgcgaggc
    27061 catggatcgc ctcttcgatg atgcgttcac acggccgctc agcatcgctg gcaatggctg
    27121 ggccgtgcct gcggtggata tgtatcagac cgataacgaa gtcgtggtca aagccgcgct
    27181 gccgggcatg aaagctgacg acgtgcagct caatgtcacc ggcgaggtgc tcaccatcaa
    27241 aggtgagatc aaacagaagg atgaattgaa agagaaagcc tatcatctgc gtgaacagcg
    27301 ctggggctcg ttcgaacgct cggtcatcct ccccactgag gttgtcgccg acaaggccaa
    27361 agccgatttc gagaacggca tcctcaccat cacacttccc aaggcagaag aagccaaacc
    27421 caagagcatt tccatcaagg cgaagtaaga tccctgtttc cagtagggcg agatattatc
    27481 tcgccctact cttttatata tggatgcaga acaggcggta tgattggggg aataggagac
    27541 tcatccacat gaccaactct ttttcacttt tgcgcatcaa ccctgaccgt atgcaggata
    27601 cgtttaatca actggctttg atcggctcga caggtgacgg tggtgtcaat cggcctacat
    27661 tcagcgaggc gcaccttgct gcgcgcaaat ggttcaaaga acagatcgaa gactctggct
    27721 tggaattccg caccgatggc gcagggaatc attctgcatt tctaacctgc caacgttcaa
    27781 acgttccaac gttattactt ggctcgcacc tcgactccgt tcccaaaggc ggacgattcg
    27841 atggcgcact tggcgtgatg gcggcactcg aagtgttgag aactgtcaaa gaaagcagga
    27901 tcaacctgcg gttaaatctc gaagcgattg actttacaga cgaagaaggc acacatttga
    27961 gtctgtttgg gagcagtgcg atctccgggc atcttcatcc agaagatttg aaaaacccat
    28021 acagcggacg tgaaaatttc accgctgggc tggcgcgtgc gggtttaact gaagcaagtt
    28081 tatttcaggc aaagcgcgcg aaggaatctt tggcgggtta tttggaattg catatcgaac
    28141 agggcaagcg ccttgagcgt gcgaagattg atatcggcat tgtttcggcg atcgtgggct
    28201 atggatcgta tcgcatgagc tacattggcc gcgccgacca tgcaggctcg acgacgatgg
    28261 aagatcggtt ggatgcgggg cagggcgttg ccgcgtttat tttggcggtg cgcgaaaccg
    28321 tgatgcgcga ttttccgaat tgcgttgcga atgtgggcaa gatcgagttt gcgcccggtg
    28381 cttcgaatat tgtccccgcc cgcgcagact ttctgctgga attccgctca cccgatgaaa
    28441 atcaactcgc agaattggat gcgactttga tgatgcttgc aaaagaaatg gccgaacaat
    28501 ttggcttgca gttggttgca gagccgctcg gcaaacacct gccaaattta atgagcacag
    28561 agattcaaga tgctttcaag aattcatgtg atgacttggg attatcgagc acagtgcttg
    28621 cttccggcgc gatccacgat gcgcaatcgc tggtggatgt ttgcccgatc ggcatgatct
    28681 tcgtcccttc ggtggatggc gccagccacg cgccgcgcga gtttaccaaa tgggcggact
    28741 gcgtgaacgg cgcgaatgtg ctgttgcaaa cagtgttacg gttggcggca gggtaaaata
    28801 tcattatgaa aaacttcgac ccgaaatata acaacattcc caaaaccgaa atccactgcc
    28861 atcttgaagg cgcgatccgt acgcagacga tcattgatgt ggcgcgcgaa tatgatttga
    28921 gcctgccttc gtacgatgtc cgcgaactgg atacgcatgt gaaggtgttc gatcaaatgc
    28981 gtaatttgga agcggtgttg gaggcgtttg ggatttttca aaacagcatt acttcgcctg
    29041 ccgttttcga gcggatcagt tgggagttgt tcgaagattg cgcgaaacaa aacatcaaac
    29101 tatttgaagt gcgtttttct cctgattggg catttcacgg tcacaacctg gattgggatg
    29161 cctgcctcga tggcttgctg cgtgcgaagg aacgcgccga aaaggaattt gatatggcaa
    29221 ttggcatcat cgccattacc tcacgcggca tgggtgcaga gtcttgtgtc aaaacagtgg
    29281 attgggcgat ccgccataaa aaacatattc aggcggttga ccttgccgat ggggaattgc
    29341 tctacccgat gagtgatttt gtgaagccga ttctgaaggc gaaagaagcg gggttgaagg
    29401 tgacgattca ttcgggggag gatacgcctg cttcagccgt gtgggatacg atccataatt
    29461 ttcaaccgga tcgcatcggt catggcattc atagtattga agatatgaag gtggtggatt
    29521 tgatcaagga gcgcggtatc acgcttgagg tcaacccctg gagcaactat ctgaccaact
    29581 cggtgcggac aattgaagag catccgctca agaagttgtt cgatttaggc gtgcgcgtaa
    29641 ccattaattc agatgacccc gaagtgttgg agacgaatgt caacaatgag tatcgaatcg
    29701 ctcacgaggt tttggggatg agcatggaag atatcgcaac ctgcaaccgc tatgcgtttg
    29761 aatcttcctt tgtggatgaa aaggtcaaac aacggatctg gcagaaatat tttatgtaga
    29821 tgtgaaaaga aaatcaaaac gggtcaggcc aaacctgacc cgtttttgat tcggcggtta
    29881 ttctttgcgg cgctgccagg aggggtggtt cttgtagttg ctttccttat tttgatacat
    29941 ggcttcatct gcgcggatga gggttttttc aagcgtttca ttctttcttc gagttgcata
    30001 tcccatggaa agctgcaact gcggaccctg ataatatttg ttgttgagag ccacaagggc
    30061 atggattcca tccaagtact cttgtggggc ttcaggattt ttcaggataa tcgcaaattc
    30121 atcgccgccg atgcgggcta tggtggcttt ttcggcaaaa gcggttttta atacttcagc
    30181 tgcgcggcgg atgaggttat cgcccgcttg atgtcccaga gtgtcattga cgtgtttcaa
    30241 accgtccagg tcgatcatga taaagctggg ttcgctttca gaagatgcga gttgaagtag
    30301 ttcatcttca aagaaggcgc gattgtacaa gccagtcatg acatcatggg tgccaaggta
    30361 tcgcatgtat tcctcggctt ttttacgggc ggtgatatcc tgaaccgcca ccatcaccca
    30421 tttgaaatcg tcttcatgtc cgggcatcag ccgccaatcc agatgcacgt tgaagggttc
    30481 accggagagc gagtagttaa tcccctcacg ctcgtaggtt gtttttccac tccacatatc
    30541 gagcagttcg tcggcgaaat gagcgctcat ttcatcgcga aacactcggt ccaggttggc
    30601 gagcagttct tctttggatg acgctccgta gagttcgagg gttttcaggt tgatatcagt
    30661 gacggcgatc ttgttcataa attggccgac cagttccggg gtctgtagaa gatgcgtttt
    30721 caaatcggtc acaccctgtg cgcgcagcgt gtcgaacatc tcgcggatgc cctggtaatt
    30781 ctcttcccaa agtgaaatgg gggcgtatgt gaacaggttt tggaaacgtt tttctgcctg
    30841 tttgagcggg gtgatgtctt cgatggctac catgacacat tcccattttt cacagcattc
    30901 tggcaggatc ttccagcgca ggcggatatt aagcggtttg tctttcaagg tatagttgat
    30961 gccttcgcct gaccaatttg tttcgccatt ccatagtgcg agcagctcgg tctggaagtg
    31021 gtgccgcatt tcgtcgcgga agatcttgtg taggttggcg atcagttcct cgacagatcg
    31081 tgcgccgaac atttccaatg ttttgaggtt gacatgggta accttgatgc gcctcatgca
    31141 ggcatccaca tcgttggggt ggtgggcgaa gtgggctttc aggtcggtta cgcctccaag
    31201 gcgcaaccct tcaaagaata gtttgatccc actgtaatcc tcctcccaga gtgaaatggg
    31261 ggcatattcg aacaggcttt tgtaatgctc aacctgattt gacatggcgg tctccttcgg
    31321 atgatttgat tatacaaaag aaaaagggca aaaggggatg gctaaaagaa tgctttgatt
    31381 gccacgagca gcaattcctc aggatccttg tgtttggtca ggtagcccgc cgctcccgcc
    31441 gaaaactgaa attgaccgaa tcggaagcat tggtgtataa ttccgcgcca ctaacttaca
    31501 gacaactccc ctgtgggatt tgcaatcatc attaaccaag gaagactttt gaatgactaa
    31561 gagaacatac caacccaaga tccgccgccg cgtgcgtgtg catggcttcc gcgcccgcat
    31621 ggcaaccgct gacggacgtg cagtgttgaa gcgccgccgc cttaagggcc gctacaaact
    31681 caccgtcaaa gccaatgagc atgtaaagaa gactcgttgg taggcatttg cgttttttag
    31741 ggcgcataga ttcacattga ggcacgggtg cagagcaaat ttcgcctgtc ccgatctgaa
    31801 gatttcaagc gggtgcggcg aactggcaag tcctatgccc acccgcttgt tgtgttggta
    31861 gcacagacga ctgagtcacc tgcccgccat gtgcgtgtgg gtgtagcggc agggaaaacg
    31921 accggtactg ccgtccaccg caatcgggcc aaacgattac tgcgcgaggc catgcgaccc
    31981 ctgctaacct ccctcgcttc cggctgggac cttgtattga ttgcccgacc ttcgcttgta
    32041 tctgctactt tagaagatac ccgctccgcc ctgctggccc tcctccaacg cgcaaaaatc
    32101 attcctacgg atgaatcctg aacctgaacg ggaattccat ttacacgatt actcccatga
    32161 gccgcgtctg cgtgacatgc cgcgtaatct ggcaaactgg ccacgcattg ctatgctggc
    32221 cttgattcgt ttgtatcaaa tggcaatttc cccggggttg cccgcaaaca cctgccgctt
    32281 ttatccatcc tgctctcatt atggctatca ggccgtttat aaacatggcg cgttaaaagg
    32341 ttcgttgatg gctgtttggc gcgtattgcg ttgcaacccg ttcaacccgg gcgggtacga
    32401 tcctgttccc tgaaaccccg gctgtggcac ttaatttagg agaagtaaat atcttgaata
    32461 caaaacgatt ggttttgatc tcattgctgg tactgggttc tttgatactc agtgcctgta
    32521 gcggcaaccg cacagtatcc aacaattggc atggccttgc tgcagatgcg gaacatgcct
    32581 atgtggccag cggcacacac atttatgcgg tgaatatgga caatggcgac gaagcctggc
    32641 gcttcccgca ggaagccgat tccaaattga tgttttatgc caaccccgtc ctcacggctg
    32701 atggtcagtt gttgatcgga agcgcgggat cctcgcaccc cttttacagc ctgaaccctg
    32761 caaatggaaa agagaattgg gttgagccat tcactggagc aaagggagcc tggcttgcct
    32821 ctccattaat cgcgaatgac accatctatg ccccaaatac tgatggctat gtctatgtgc
    32881 tggatctgga gggcaaacaa gccgccgacc ccattcagat cggtggcgca ttgtggtctg
    32941 cccctgtaac agatggcaag ttcatttata tcgcttcact cgatcaccat ctgcacatca
    33001 ttgacccatc tgacggcagc actgtagata ccattgacct gggtggagcc gttcccggca
    33061 ccccggcggt ggctgaaaat ggtgtgtatg tgggttcgtt cgcatccact gttgaattca
    33121 ttcaatccaa cggcaatcac gaggtcgttg ctaaaactga aaattggatc tggggttccc
    33181 ctgtccttgc aggtgagacg ttgtactacg ctgatctcac tggcaaggtc ttttcattca
    33241 acacaaccga gggaagccaa aactgggaaa ttgccctgcc cgatgccgtc gtggctggcc
    33301 tgcttgtttc tggcaaccag atctacgttg cgaccgaagc gggtaacctc atcgcattgg
    33361 acttggatgg aaacaccgtc tgggaaaaac ccgtgggcgg caaactctac accgcgcccg
    33421 tgctttcagg cgaaaccatc ctcgttgcac cgtaccaggc cgaaaccacc ctcgttgctt
    33481 atgatgtgga tggcaaacaa gtctggccgt tcacaccgga aaaataagga aaccgaccca
    33541 tgtgggatac tattattatc aaccccatga ccaacctgtt attgtggatc tatgacgtac
    33601 ttggtcatgg tccgcacatg tttggtcttg cgatcatttt gttcaccatt ctcatcaaag
    33661 cagtgacctg gcccttgaat gcctctcagg taaaaggcgc acaggccatg caggaactga
    33721 acaacgataa ggaatggcag gagattcaaa agaaatacgc caaagaccgt gaaaagctcg
    33781 cgcaggagca aatgcgtatt tacaaggaaa agggcatcaa tccttttgcg tcctgtctgc
    33841 ccacgttgat ccaattcccg atcatcattg gcctgtatca aagcattacc cgcgcgcttt
    33901 ccatcacccc ctttgacatg ctcaaactgg cgcgcaccat ctaccccttt caaaacgttg
    33961 agaacatcat cccgctcaac agcacattcc tgtggatgga cctgggccgc cctgagggta
    34021 tcttccaaag catcattcat acatccttcc tgcccaacgg gtttccgctt cttgcactga
    34081 tcgtggccct caccacctac gtgcaatcga agttgaccat gccgacctcc agcaacccaa
    34141 atgaccaatc ggcgcaaatg tcgagcatga tgagcatcta catgccgctc ctgcttggct
    34201 ggttcgcact gaattttgcc tcaggcatcg ctatctattt catcaccagc aacctactcg
    34261 gtatcattca atatgcagcc acgggtcgcg ccaactggag caacctgctg cccggcagca
    34321 agaacaagta aaagataggg aggcactatg agcggaacca cgcttgaaat catcgctcca
    34381 accgttgaag aagcccttgc acagggcctg gcccaactcg gcctgacggc agatgcagtt
    34441 tctgtggaag tgctcgattc aggcagcaag ggcctgtttg gactcggcgg aaggcaggta
    34501 cgagtacgcc tgaccgtgaa cccgccgcct gttgaagaaa cccacacccc tgcacctgcc
    34561 agacaaaaac ctgcggatcg tcccaagggc aagcctgctc ctgctcaaaa agcagaatca
    34621 gcgccgaagg cccagcgcga accaaaaccc cgcacagaaa agaaacccgc accggaagcg
    34681 cctgccccag taaaacagcg ccccctctcc gaaggtagtg acgccctgct cgataccacc
    34741 gagtctgtca tttcgaaatt gattcatcac cttggcatga aagcacaggt ctcggctcat
    34801 tacgatgaat ccagcaccga ggaccgccgt gtcattcaag tggatgtacg cggagatgac
    34861 ttgagcgccc tgatcggccg ccaggcagaa acgctgaacg cctttcaata tgtcgcatcc
    34921 ttgatcgttg gcaaggaaac ccagaagtgg gtgcaactgg tcgtggatgt ggaagggtac
    34981 cgcagccgcc gcgaaaagca ggtccgccag atcgccttgc gcatggtgga tcaggtcatc
    35041 aagaccgggc gcaaagccac actcgaaccc atgacggcca gcgaacgccg cgccatccac
    35101 atcgagttgc gcggacatcc cgcagtgacg actgaaagcg tcggcgaaga accgcaccgc
    35161 aaagtggtca tcctgccaaa agaataaccg tctgtaggga cacagcgccg ctgtgtccct
    35221 tttttattca ccaaaacagg acacggcagc gccgtgtcct tacatttccc tacggtataa
    35281 ttcgtccatg ctcgaacttg tatcacttca acctgtggat tatctcgttg taggtcatgc
    35341 cgctgtggat atcaccccaa cggggatgca gcttggcggc acggtttcct actcgtcgtt
    35401 gaccgcacgt gcgttgggga tgcgggtggg aattgtcacc gcggtggggg agggtgcgcc
    35461 catgcaggcg ctggatggca tcaccattgt caacattccc tcggaacaca gcaccacttt
    35521 tgaaaatatc aaaaccgatc acggacgcag gcaggtattg catcatcagg ctgcgccgat
    35581 cacattggaa catattccgc aggtctggcg gagtgcgccg atcatacacc tcgcgcccat
    35641 cgcccgcgaa ttggacccat ctttgcctga gaaactgact ggctcattgt tgggaatcac
    35701 gccgcaaggc tggatgcgcc aatgggatga aaatggtcaa gtgacggcct ctgcctggga
    35761 aaacagggat caggcccttc gttatgcagg agccgtggtt ttgagcgtcg aagatatcaa
    35821 tcgcgatctt gagcttgtcg aagaaatggc gcaccagacc cgcatcctct gcatgacgga
    35881 aggggagttg ggttctgtgc tgcattggaa tggcgaccgc cgccgttttc gtccgtctgt
    35941 gatgcatgaa gtggatgcga cgggcgcggg cgatattttt gctgcagcat tttttatccg
    36001 tttatatgcc acgcgtgacc cgtgggaggc ggctcgtttt gccactcaac tcgcggcccg
    36061 ctcggtgaca cgggtcgggt tgaacgggat cccgaccaaa gatgagatcg aagaatgttt
    36121 gatggaggta taccagtgac gcgtatttat acgcttgtga atcaaaaagg cggtgtgggg
    36181 aagaccacca ccacgatcaa cctcggtgcg tatctggcgc agttgggcca gcgtgtgctg
    36241 gtggtggatc tggacccgca ggccaatgcc acatcctgcc ttggcgtgga taagttgagc
    36301 gttcagggcg gtacctacga agccctgttg ggcgatacga gcattgcttc ctatgttttg
    36361 ctgaatgaga agctcaaatt atccctgctg ccttcttcgc cgtcgctcgc gggtgcggaa
    36421 gtggaattgg tggatgaact tgcccgggaa gtgcgtttgc gcagggccct ggagcaggtt
    36481 gccagtcaat atgattatgt gttgatcgat tgtccgcctt cgttggggct gttgaccgta
    36541 aatgggttga tggctgccat caacggtgtg atcgtgcctg tgcagtgtga gtacctcgcg
    36601 ctggaagggc tggggcagtt gacccaaacc ataaaccgtg tgcggtcttt gcttttccct
    36661 gagttgaatg tgcgcggcgt ggtgctgaca atgttcgata accgtacaaa cctttcaacg
    36721 gatgtggttt ctgaggtgaa tcggcatttc cccgatcaag tgttcaagag tgtgattccg
    36781 cgcagtatcc gccttgcgga agcgccttcg tatggcctgc ctatttcggc ttatgcgccc
    36841 acttctgtcg gcgcggcagc atatgaagcg ctggcgaagg aattgttgaa gggtgataaa
    36901 aactaaccac aaagtatcac gaaggaaatc ttcttaaacc tttgtgatac tttgtgaccc
    36961 ttggtggttg aaaaggtaaa aactatggct caaaagactg gacttggcaa aggtttggat
    37021 gcgctcattc ctgctggtgg gaaaaccccg cccgcttcat caagcggggg aggcacgggt
    37081 ggtgttcagc aagtggctgt ggaagccatc aagccgaatc cgcgccagcc gcgtgtgcat
    37141 ttcaaggaag atgaattggc agagttggct gcgtccatcc gtgaacatgg tgtgatccag
    37201 ccgatcatcg tcacccccaa agcggatggg acctacctca tcattgcggg ggaacgacgc
    37261 tggcaggctt ctcaaaaagc tggcttgcgg actgtccctg tcatcacgcg ccaggcgaac
    37321 aatcaggaat tgcttgagct tgcgttgatc gaaaacgtcc agcgagccga cttgaattct
    37381 attgaagaag ccgaagccta ccgtcaactc gttgaagaat tcgggctttc gcatgaagcc
    37441 gttgcgaaac gcgttggcaa aagccgcgta gccgtgacca ataccctgcg cctgctcggc
    37501 ctcgcggacg tggtcaaaca ggccctcgta gatgggaccg tgaccgaagg ccacgcccgt
    37561 gcattgctgg ccttgtccac gcagaaggcg caggcttccg ctttgcagac gatcatcaac
    37621 ctctctttca gtgtccgcca aacggaagag tacgtgagaa aactcgcggg gcagaaaccg
    37681 atcaaagcga agaagcccaa ccgcagtgca gatgtggcgg atgtggaaaa gcgattgcaa
    37741 aacagcctcg gcaccaaagt cgccttgaag cacagtaaaa aaggcggcac ggtcaccatc
    37801 tactattact caggcgaaga actggatgcg cttctcgaaa aattgatcta acccgtaggg
    37861 acacagc
//
LOCUS       DAOQJC010000023        37796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_466_length_37796_cov_8.131025, whole genome shotgun sequence.
ACCESSION   DAOQJC010000023 DAOQJC010000000
VERSION     DAOQJC010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 37796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37796
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_466_length_37796_cov_8.131025"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLE39_05165"
     CDS             <1..474
                     /locus_tag="PLE39_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67748.1"
                     /translation="HFTGFGLYDQKVIDILKGLQDPYPYFRGLIADIGFEPAIIEFTQ
                     PARKFGKSKSNLFYLYEEAMLGLTSYTKLPLRLATIIGFSSALISFLIGMFYLVYKLL
                     FWSSFSVGTAPITIGLFFFASIQLIFLGIIGEYISMIYIHVLNRPLVYEKERINF"
     gene            471..1508
                     /locus_tag="PLE39_05170"
     CDS             471..1508
                     /locus_tag="PLE39_05170"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNE67749.1"
                     /translation="MNQQSTPVPLPEDLVDEKPKRTSRLMLLVSVILAGVFLYFALRN
                     LDWQVFFAALKGARYSYLPIILFLININYLFRAMRWRGLLLNEKHISVFTVFWANMTG
                     YLGNNVLPARAGEVMRAAYIARKENISISFIIATGVTERLVDLAALVIVGAVSLFFVD
                     ALPRSVQDALKSFAIIALAGVVFLFLLPYFHGIFSRILASLSFLGDSLKTKIQEFTSR
                     FIEGIKVISRAERGLPFIFFTALIWLLDGIGTVFFALAFNESLTLMQAFLFIAALGLS
                     SAIPSTPGYVGVYQFVAVTVLAPFGIKRESALAMILVSQALNLLIVSLWGGLGLWFGS
                     QGMVKSTPQGN"
     gene            1511..3625
                     /locus_tag="PLE39_05175"
     CDS             1511..3625
                     /locus_tag="PLE39_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67750.1"
                     /translation="MDSNFFLQDIVGISLAFLLFSFVFIFPGYVIGWGFNLLKHRSQP
                     LMLQLGTGIFISISVIPAFLFLVYRFLPAKIGIGILFAFAIAAIYIYFTQNKASGNNA
                     VPASEKGYQKSVLGLILVWFLFSVFVSADLQIGKRLYFSNNSYDMLTRVAVVDAMTRT
                     GVPPVNPSYYPGEEVRLNFLYYYWYILASVVDMLGGPLVSALHAMIASISWCGIALFA
                     TVSTYMQVRAPSENAWKKTFIGIQLFAVSGLDCIIIILAAVREGYLPVNGGIEGWNMP
                     IMSWLNAITWVPHHVAAAIACIFALLLLLHEKNERLPAKLLVSVMIGLAFASAFGLSV
                     WVMFVFAFFWAVWAIISLLEKKYASLGVMVLSGFFALIFISPFLAGLVSPTGETSTAS
                     SASPVAFYIRPFMASVVFPEGTPSIVLGTANFFMLPLNYLFELGFFFLMAAIWIQEFY
                     SRNKNTNPIYKAELVLAAVTTVVLSFLYSTLISINDLGIRGWLPLQIILIVWAVDVIT
                     MFMGNEKWITPRIFRVFQNPKSLGLVLGVTLTLGLLTTSLEAISLRTWAILIDMNVTD
                     FPNSISVDNHLGERTYYARLAYSYISENTPNNIIIQSNPQNQMERPSGLYATRQMVIS
                     DRTIYGVPIPVYAKLSQDIGSIFRRKTSSWDDIDTKCTQYKIDILVFKDTDRVWESLP
                     DLKTIRPPLYENPYYALYSCGK"
     gene            complement(3628..>5341)
                     /gene="mutS"
                     /locus_tag="PLE39_05180"
     CDS             complement(3628..>5341)
                     /gene="mutS"
                     /locus_tag="PLE39_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNE67751.1"
                     /translation="RKGSLLGTLDFSVTPMGKRLMHQWVSQPLLHVAKITQRQNGVQY
                     FVENGMVRAELRATLKPLADLERLVNRVLAGQAQPRDLVAMRTTLTELPKIKEVISGQ
                     KAVVSDQWSVCTQELSLLQNAIDDDPPATLQNTGVIRPGYSSELDGVIDASKHARDWI
                     ANLEGVEREKTGIKTLKVGYNKVFGYYIEISRGAADKAPESYIRKQTLVNAERFITPE
                     MKEYETLVLNAEERIKEIETRLFREVCAELVKAANQILATARAIAELDVLSALGEAAA
                     LGGYTRPEVHEGSGLEIHEGRHPVVEQLLKSDRYIPNDVIFEKGEVVRVITGPNMSGK
                     STYLRQTALIVLMAQMGSFVPAASAKIGLVDRIFTRIGAQDEIHAGQSTFMVEMVEAA
                     NILHHATPRSLLILDEIGRGTSTYDGLSIAWGIIEYIHNHPQLRAKTLFATHYHELTQ
                     LADLLPGIRNYNVAVSEADNKVVFLHKIIAGGADRSYGIHVGQLAGLPAPVIQRATEI
                     MAELEKTSGRAVKIDPNAAQQVALFPETNPLLDELKELDVNSLSPIEALNKLFEWRMR
                     FTKS"
     assembly_gap    5342..5441
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5442..6301)
                     /locus_tag="PLE39_05185"
     CDS             complement(<5442..6301)
                     /locus_tag="PLE39_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNE67752.1"
                     /translation="MPSNDDLTPVRQQYLEIKREHPDRIVFFRLGDFYETFDEDAEIT
                     ARELDIVLTSRPVGGGVRVPLAGIPYHAVENYLARLIEKGYHVAICEQVGEQPVKGIF
                     PRKVVRVVTPGTVTEPGLLPGDANNYLASVILDTTTAVNSNPITASVSYVDITTGEFA
                     VTELPLEALRAELTRLHPAEIIHPDSQVLPDGITGHITALPAWKFEPGKCSEVLISHF
                     KASTLAGFGLKDNSLSARAAGAIVQYLKETQPDALNLLTSLRAYSLNEFMTLDASTRR
                     NLELDETLRG"
     gene            6412..6939
                     /locus_tag="PLE39_05190"
     CDS             6412..6939
                     /locus_tag="PLE39_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67753.1"
                     /translation="MFNHRLPKLNDVLSVYAVIATMLFAWSALLFFWYLPSWMHFMLL
                     GEIAATFSYVVIASFLEGLSFLLFLLVLCFFLPPEYLRDEFAARGTALTLPIIGMIMI
                     YFRITNENIARIIFSVTGLVISLVVILLFYFSANNPRIKSAFAALAERFTVFLYILIP
                     IFVLSLLGIALRNIF"
     gene            6949..8481
                     /locus_tag="PLE39_05195"
     CDS             6949..8481
                     /locus_tag="PLE39_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNE67754.1"
                     /translation="MKSLSRRDFLKTAAAFSAGSLLSMAPALQNIKAQSNKPNIIVMV
                     FDAMSARNLSVYGYPRSTTPGLERIAERATVYHSHYSAGNFTTAGTASMLTGLYPWTH
                     RGINLSGMVNKNHVDHNIFNLIGDEYHRTAFTQNYLADVLLGQFQSGLDRHIPAPTFY
                     DQLDHPLIGQYFPRDPIPAYYGLDDYLFSGGIDRVGSIPSAPIFGFLNKLARRNQDFN
                     KSLPDYPYGIPNNTFYSYKHEVVYAGVLDEIKNLASEQNPFLAYFHLFSPHEPYRPRK
                     QFTDIFPELEVPFKAPTKFSTLDIDNDHILWRRKTYDEFVADVDSEIEKLVNALDESG
                     ILENTYLILTADHGELFERGEYGHVTRLLYDAVVHIPLIVIAPGQKSRVDVHHPTSNI
                     DLLPTLLHIAGKTAATDLEGRILPGLGGIEESRSLFSMEAKECSAFGNLKDGATVSLI
                     QGEHKLIYYTGYPKRPDTFELYNLKDDPDEMKDLFTVDTTTAAKMKEEMLDTFESNRK
                     LK"
     gene            8553..8714
                     /locus_tag="PLE39_05200"
     CDS             8553..8714
                     /locus_tag="PLE39_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67755.1"
                     /translation="MYIDPNTGGILFQALAAVFVAGSGVLFFFSRNIREGIAKLRRRF
                     RKEDEEQNQ"
     gene            8724..>10522
                     /locus_tag="PLE39_05205"
     CDS             8724..>10522
                     /locus_tag="PLE39_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNE67756.1"
                     /translation="MKTIILGFDSFDPAVFEDMAGQNKLPNFSKFMDAGGYSRLEVCS
                     PPQTEVSWTSIATGADPGGHGIFDFVHRDPATYAPYVSILPMKKSAMGEQFVPPYIAK
                     TLFEEAASMGYPATALWWPAMFPARPELPVNTLPGLGTPDIRGQLGVGTLFTTEDEKK
                     SKTAVTRFESTGKGRYSALLNGPQVQGKNGPQPATLPIQLEALDQTSARVTIGDQQIQ
                     LRVGEWSDIVEVKFKAGLFLSIHAITRMILTSLNGVVRLYTLPLQIHPLHALSHYSSS
                     KSFAKDLWQKVGPYLTLGWPQDTTGLEDGCITDEQFIALCDLIFERRKQIFFHLLGNF
                     KEGVLGSIFDDLDRVQHMFFHNRLDVAQAWYQKLDAFVGEVSQRVEKWGGKYNYLIMS
                     DHGFTTYQQKIHLNRWLAENGYLATSNGSAEGDLSNMDWQKTKAYAVGLNSIYLNVAG
                     REGKGVVGANEIESLLAEIQQKLMGWNGPDGRPVMQRVRFKHEVYNGAYTRLGPDLVI
                     GYAPGYRASSETGLGKVPQNLVEANHDHWGADHCVDSDVVPGVIFANRDLQNFGGVSF
                     RDIPFLAIGKHLDQSHIKPPSQVSGGSQKDLEDR"
     assembly_gap    10523..10622
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10623..11245
                     /locus_tag="PLE39_05210"
     CDS             <10623..11245
                     /locus_tag="PLE39_05210"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNE67757.1"
                     /translation="SGNPLKNLFSRQTPKKTNEKIVVVSGLPRSGTSMMMKMLAEGGL
                     PTVVDSLRQADEDNPNGYFEIEQVKALKDGEKNWIYDARGKVVKVISYLLEYLPNDLE
                     FNVIFMDREIHEVLASQKKMLEHRNEVSAISDEDMEKQYREHLKAVRYWLPRQPNMRV
                     LYVKYDQMVKSPQTLVPSIIEFLERPLNAEAMAAVPSPTLYRNRKP"
     gene            11296..12042
                     /gene="gpmA"
                     /locus_tag="PLE39_05215"
     CDS             11296..12042
                     /gene="gpmA"
                     /locus_tag="PLE39_05215"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNE67758.1"
                     /translation="MYKLVLVRHGQSTWNLENRFTGWTDVDLTDLGRSEAREAGKLLR
                     EGGYDFDIAYTSVLKRAIKTLGLVQEEMDREWLPVVRAWQLNERHYGSLQGLNKSEMA
                     EKFGEEQVKIWRRSYDVPPPALELTDERHPKFDRRYASLTPEQLPATESLKITLDRVL
                     PFWHSTLAPEIKSGKRVLIVAHGNSIRAMVKYLDNISEAEITELNIPTGLPLVYELDE
                     ALKPIKHYYLGDAEEAAKKAAAVANQGKAK"
     gene            12133..12690
                     /locus_tag="PLE39_05220"
     CDS             12133..12690
                     /locus_tag="PLE39_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67759.1"
                     /translation="MKLLKLISERMPDSKNILAVYAFIVMLVYSWTLFTSFYKLPSWL
                     FYLSIGQILSVYAYAFSFDLLESLIALTVILLLDYTVFLFLKNKEEFQSRSMILAGIL
                     LGTSMTRLALFQNYEDITAFLSGELIWWAIAIPLGLVLAAFAPRIKLVRTIFEGFAER
                     ATVFVYIYIPLSLISLVVVLIRNIN"
     gene            12699..14240
                     /locus_tag="PLE39_05225"
     CDS             12699..14240
                     /locus_tag="PLE39_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNE67760.1"
                     /translation="MTNFSRRDFLKLLGAASVGALASERVLSLKAADNSKPNIIIILW
                     DAFSARHLSLYGYPRQTTPNIDAFAEYSTVYHNHYSGGNFTTCGTASMLTGMIPWKHR
                     AINYDGLVRAEYIHNNPYTLLGSDYKRLMFAQNPWPDRLVGQYYEDVDRFLPPTAFSL
                     LEDSPVFFENDRAIASVAMSSFLMPIQGGNAPVGSSILGYMNKSRVLNFADGQKSPRY
                     PKGIPEAMNMGYMIPYLNEDVYEGLFSEMTQLEKNGDPFFSYFHLYSPHYPYRPRNDY
                     LKMFRDDGFAPVSKPIHPLSVGLQQDYLNGQRDMYDRQIAQIDEEFGRLIPRLEESGV
                     LENSYIIFTADHGELFERGFAGHGFQFMYESVLHIPLIIHAPGQTKRQDIHALTSNTD
                     ILPTILSIAGKETTPDMDGRILPGLGGEVDEDRPIISLVGVDNSAFGVIKKAVIAMRK
                     REFKLIAYLGYEDSYKKSFELYNIEADPEELDDLSDRDTATLSAMKDELLTYLDEANK
                     PFRKS"
     gene            14248..15789
                     /locus_tag="PLE39_05230"
     CDS             14248..15789
                     /locus_tag="PLE39_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNE67761.1"
                     /translation="MAQITRRDFLKLAGGALAGSLVSSKLTSLAQGDKDSPNIIIILC
                     DALSARHMSVYGYPRETTPYISAFADRATVFHQHISGGNFTSTGTASLLTGMFGWKHR
                     AINQGGLVLPEYAKTNPYSLLSTDYFKFAFSQNPWSDRLIGQNSRAVDKFLPVTSYSL
                     REENPISRIFKNDRTLASIALDDFMIPVQGDTPGSSIISAYYKKKAMDTFLKDKNKSA
                     RYPDGLPEILIGGYVAPYLNESVYNGVYEEVSQLEASSQPYFAYFHLFSPHFPYSPRR
                     EYLKLFRDDFSPPLKPIHPLSPRLHKDFIASRRTAYDRQVAQIDEEFGRLIAKLDKLE
                     ALANSYLIFTADHGELFERGFSGHGFQFMYEGALHTPLLIHAPGQASRVDIHTPTSAV
                     DLLPTLLCITGKDIPTDIDGKVLPTFGGDEDTERPIFSVYAVDNSAFGPLKKCVIAMR
                     KGAHKLIAYLGYKNYDEVFELYNIETDPEELFDLAAKELDLLSKLKQEFKAHLEEANK
                     PFAKT"
     gene            15859..18813
                     /locus_tag="PLE39_05235"
     CDS             15859..18813
                     /locus_tag="PLE39_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNE67762.1"
                     /translation="MTLSPDFLHELKKRFTGDLRLDPASRTLYSTDASMYQIEPLGIA
                     IPKTQDDLQAAVELAAKYAIPILPRGAGSSLGGQAIGEALILDCSRHLDSILEIDPDS
                     QTATVEPGVVLSTLNAASAKFGLTFGPDPASAERATMGGVIGNNATGAHSILYGMSAD
                     HLVSADVIMADGSLAVWNKIVLEEREKRKDDSLFSSFLSTALDIREKYSKAIKQNFPK
                     TWRNSAGYRLNYLLPWSPSAPPQWIGDSYPANLQPSIFNLATLLAGSEGTLAVMQRAT
                     VNLVKKPKHTILGVLAYQSIADACDDVPRLLEFKPSAIELIPQMIIQLARGVPSFARQ
                     MGWMTGNPAAVLVIEFSGDQPSALKEAVRRVGNVITIAESPEEQARVWNIRKMGLGIL
                     DSRPQAARPAAFIEDCAIPVERLGEFVREIERILRDHHTEGGIYAHASGGCLHIRPIL
                     NLHLGEGVRALRSIGGQVLQLALRLGGSMSSEHGDGIATGEWIEKTYGAEVTKAMRTL
                     KHAADPRNILNPKKMFDAPPMDTNLRYGENYQAKTWVPSLHLDHERGLAGAIEHCNGQ
                     GVCRKTNGVMCPSFQATRDEANSTRGRANLLRGLITDYHLPSAENARQKELMQATYQA
                     LDLCLACKGCTSECPSGVDMPKLKYEFMNEYYKTHRRHLRDYLFGYFHVVSKWLAPAA
                     PLANAFMQMSWAQKLIARTTGITEKRPFPLFAKRSSAQKAYPNAWKNGKAVIFLSDVF
                     SRYLEPEVEQAAFELLHAAGYEVKTLPIIGSGASLLSKGFVDAARKHAEKVLSEIHAL
                     DPDSTLSVVGCEPPEVYCLKHEYSALLPNRRTELESISRRVWLVDEFLLRDENLERLV
                     QMGETAPHSEVIHFHPHCHQRAEGLSEDGLPNGTAATTELLRRFGFQVNLLDTGCCGM
                     AGTFGYDANHYELSMQVGELKLFPKIRELGSGNGDLASSGSACRLQIKQGVGKTAEHP
                     LVVIAKRISPNG"
     gene            complement(18874..19314)
                     /locus_tag="PLE39_05240"
     CDS             complement(18874..19314)
                     /locus_tag="PLE39_05240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE67763.1"
                     /translation="MANGGEFKIELFADKAPITVNSFVFLTCKGFFNGLTFHRVLEGF
                     MAQGGDPTGSGMGGPGYEFVNEYSDLTFDKPGILAMANAGPDTNGSQFFITFGPAEWL
                     NGGYTIFGQVVSGMDVVNSLTLRDPQQNPSFIGDAMESVTISQE"
     gene            complement(19623..20255)
                     /locus_tag="PLE39_05245"
     CDS             complement(19623..20255)
                     /locus_tag="PLE39_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNE67764.1"
                     /translation="MSYVSFEVRVCQSCGLRYPITIGHSFGVRCPHCLGETRVVLGRQ
                     IQKEAVVDEERSKPKEMKAVLLDNIRSAWNVGSILRSADGFGFSHAYLCGITPTPEVD
                     AVRKTALGADEFVTWSHHKDSVKLVTGLKLEGWKVVALEEDERSIPISRYAEFQFPSP
                     VVMLLGSEVTGVDPELLELADAVFHIPMHGQKRSFNVAVAFGVAGFSLSS"
     gene            20341..24270
                     /locus_tag="PLE39_05250"
     CDS             20341..24270
                     /locus_tag="PLE39_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE67765.1"
                     /translation="MQTTRQLVPEFIYDKFRNNEISGVFQGAAMFVDLSGFSTMTDAL
                     SAHGTHGAEVLANMMRVVFEPLVNAVYEQGGFVIGYAGDAFNAVFPTEQDAGQTMRRC
                     LAAAVQMQAHAKANQLASTPYGDFSIRIKVGIGYGQVTWQIFKSINGKRASYWFRGES
                     LTGAVLGEERAYAGEIVADPISYKLISDIVEVRPVQECFLIEKVIADLPAALPVPTPE
                     PADDLASIFLADTIAHLPISGEFRQAMNLFIDIPVNISDQALITPFMETVYLLQEQYG
                     GVFLRPDLGDKGFNLLMFWGAPIAYENDVDRALNFIIELATRTRITLRAGITYHMAYA
                     GFIGSSKREDYTAYGWGINLAARMMEHAGQGEYWMSEEVARRAEKHFNLNFLGEHHFK
                     GFKQDQKVFSLLGRKNIVETVYQGQLVGREKEMENLRSFTEPIHRGNFAGVMVLQGEA
                     GMGKSRLIHSFQFSEYFTEHPARWVVCQADEILRLPFNPFKDWLKKRFELLEGQPDEI
                     NAIAFNRNLQELANITPDPELAAELIRTRSVLSALINITQPDSLYESLDAKGRYDNTL
                     IALSALLRAESLQRPLVLFLEDTHWMDEDTGNFLSYFMRTLLAESEKQYPIAIITTQR
                     PEGNANWELDGTYAHHIKLEKLPAASIAHLAKNIVDHPISASLLELLISRTDGNPFFA
                     EQVLRYLTEHNALSLNEEGEYIANQAENISLPTDVRAVLVARLDRLTKQVKEAVQTAS
                     VLGREFEIRVLGLMLKNDKRLLSHVAQAESANIWTPLSEIEFIFRHALLRDAAYSMHL
                     VARQRELHGLAVSAMETIYHEDLEPHYGELAYHAEKAALNEKALHYLNLAADSASQAY
                     QNAQVIDYLTRAHNLLPQNSQHERFEVLYKRSIAYSIIGNRSSEDMDLTALEETASAL
                     RDNTKLARVLMRRTYYFATQGENFMAISFGERAATLAQSVNDMETLGEVYNFLPSAFT
                     RVGSLKEAFDCAQKALKLSQSTNNRLGEGNALTSLGLIALENKGPSEAYQYQANALKI
                     ARELGNRDLEAKVLNNLANLIGLSNGDYSTARDYFEQAQVIYQEQGRMYPSGLALTNL
                     GWVSNMLGDYETALTYHTRALTIAREVGNKTQEMYTLINLSASTFGKGNNPNAAIYWA
                     EKALWLSEKIADQIGTAWSFFYLGYGYMLGKQYPAARQAFESSLKIREGLDAKVLTAE
                     SQAAIGQTLYELMDIEAAFIEVEKVLEYMQENPNLEGAEEPLRVMLTCYLVLKEKRDP
                     RHKNILQNANLLLDTQVSKLRSDEARHVYVENVPWRRMLRKYYKETQ"
     gene            complement(24274..24660)
                     /locus_tag="PLE39_05255"
     CDS             complement(24274..24660)
                     /locus_tag="PLE39_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67766.1"
                     /translation="MQNKVVVPGKVKKDKDNNKKYKIEKDKVSESDITIDIVDDGDYD
                     VEKLSVEGLPANMVDGNPITWINNFSIKKNGQYINQRFFVTIPNIGSSKLIIFDGNGN
                     PYYYTGEIKNNRFELTDGDPAIGHWP"
     gene            complement(24777..25610)
                     /locus_tag="PLE39_05260"
     CDS             complement(24777..25610)
                     /locus_tag="PLE39_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67767.1"
                     /translation="MRNSQLDSANPPISSLLRGITWLEVLVLIVTGGGLFFLPALARE
                     QWPWVIAPFNGRFVGTVYLGSFVSAGFMAFYGRWSPGRIVLPMIFIFTTIVLGVSLFH
                     LDQFDLQRWQPWIWFLLYVVLPLNSAYHLWLYRAWAPAESTPTPATWRNYALGLSALF
                     GLYGLALLIAPTAVSAFWPWPIDAFHGQMYSVVFITPAVGIFLITRQASKLEWKTLGL
                     TLLVIGACAIIGLLLVDAVVPPEKKVNWAIAETWTWMALMTLYGASGAAMILRSGGKN
                     P"
     gene            complement(25682..25921)
                     /locus_tag="PLE39_05265"
     CDS             complement(25682..25921)
                     /locus_tag="PLE39_05265"
                     /inference="COORDINATES: protein motif:HMM:NF021429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HNE67768.1"
                     /translation="MNSDRPKNIPDGITLEMIVTQLSEYYGWAELGRRIEIKCFTNDP
                     SIKSSLKFLRKTPWARKKVEDLYLDSLHKMNPRSG"
     gene            complement(25934..26746)
                     /locus_tag="PLE39_05270"
     CDS             complement(25934..26746)
                     /locus_tag="PLE39_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNE67769.1"
                     /translation="MGKKAIVFCVIGLFVVSCAAPQSLVTSTPDVTPTVAASFTPSPV
                     PTITATAYLTPTPPAALVTSGDWTLPYVALTFDMCQDPLYPAGYDAGIVDVLQRYNVP
                     ATFFMGGDWMRTHPDETRLLGANPNFELGNHSWSHADFTTLTAEQMGQEIDMTEDMMF
                     QLTGKRSHLFRFPSGFYNDTALQIVSEHGLYAVQWDSVTGDPDPTFDAATILAEVQRT
                     VQNGSIIIMHGNGRGWHTAEALPLVIDYLQSKGYTLVTVSQLINLEPLPAAE"
     gene            complement(26796..27584)
                     /locus_tag="PLE39_05275"
     CDS             complement(26796..27584)
                     /locus_tag="PLE39_05275"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HNE67770.1"
                     /translation="MKTTKSLIGIFVFIAVLLVTGACSGNATTTSTPLEITPPDVSTN
                     TPTAAPVLQTTETPIVPTVDPVYFRDDFDDILQPGWTWLREIPGQWSLDKAPGSLRIA
                     GGRGSVNAETMTNILLRPAPAGDFQIETILTFEPDQNFQFAGLVVYQDNNNFIQAGQG
                     YCRGGGCVSRGLYMNYYGRGLIVPPNFAQSYKNNAVVLRLTRKATSYIFEISGDGKVF
                     YKVGAHESDMTPLQVGVLAGQNVDGISTTIPALFDYFEMSSPAP"
     gene            complement(27698..28657)
                     /locus_tag="PLE39_05280"
     CDS             complement(27698..28657)
                     /locus_tag="PLE39_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNE67771.1"
                     /translation="MLRSFFIYLSKAAWAQNLITNWSFAWRAASRFVAGTNLSDAVRV
                     VKELNAKGINATLDHLGEHTNTPEEAQQATDDIFATLDAIGAEATLRGNVSIKLTQIG
                     LGLDENLCAENLERILARARQNNTFIRVDIEDTPYTDKTINLYYMMREKGYTNVGMAV
                     QSYLYRAEADTRRLTQDQITIRLVKGAYKEPPEKAFPRKADVDANYDLLTKILIDASL
                     AFQTKLSADGRFPAIPAIASHDEKRIAFAKQYADKVGLPKDGLEFQMLYGIRRDLQER
                     LVKEGYPVRVYVPFGTHWYPYFMRRLAERPANIWFFISNFFKG"
     gene            28803..30257
                     /locus_tag="PLE39_05285"
     CDS             28803..30257
                     /locus_tag="PLE39_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNE67772.1"
                     /translation="MSILFLTAALVLSIISLITYSRQRNNYPASMTIAGVPVGGLTPV
                     EASQRLLEVYTSPVELLYNDAIIHIEPAVVGFEPDIETMLAAADLSRTGGSFWGGYWD
                     FLWNRTPSEIAIPLSPKFSEERLREYLQNEIAARYDVPPSPAVPVPGGTDFLPGQPGQ
                     TLDLERAVLLVNDALRSPTNRSVALTFTRGSVARPTIDNLTILLKQIVQVSDFDGLIG
                     LYMMDLQTGQEIHFAINNKEEISVTPDIAFTASSTMKIPVVASYLINRGSNLSPDIAN
                     SISQTLGKSSNDATDVVLSAIEPNRGPVIVTENMRSIGLESTFLAGFFTSPAYLLPAI
                     PITPANSRTDVTTEPDSYSQTTPSEMGSLLADIYQCAQSNGGALIAAFPDKVSPQTCQ
                     LLIDFMAQDKLGSLIQGGVPDGTLVPHKHGYVPAADGVVHDTSDVGIVYTPGGNFVLS
                     VYTYHPVTNIWDITNPLIGDITKAIYNYFNVTSQ"
     gene            30343..31041
                     /locus_tag="PLE39_05290"
     CDS             30343..31041
                     /locus_tag="PLE39_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67773.1"
                     /translation="MSASLGLFRLQQVDRKIDRARSQLETIRKTLENDTELRECLTHA
                     ETAKASHHHAHHNLKSAEMEVESQKIKIEQADSSLYGGRVQNPKELQDLQKEIVSLKK
                     HLATLEERELEAMLAAETAEQNLQAASTQLELIQARLGSEHTKLLADQNALVAELEQL
                     IEEREATLSPLDTSLLQIYEDLRQQKRGIAVTEITDNSCAACGGTLNSAIQQSARSQK
                     LANCPTCGRILYSN"
     gene            31051..33600
                     /locus_tag="PLE39_05295"
     CDS             31051..33600
                     /locus_tag="PLE39_05295"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE67774.1"
                     /translation="MSRFAIDPYSFFVGFIAASIFWLLVSRARPLWAELVAGIKEQRE
                     IAQTRKSSTVEENHRRITLRRAQGMHLAAPLFSLDEIILEPLLIAPPQVVQPGTPAKF
                     EDVVSQTLPYMPMWPEIAAAYHAQTLTLTQALSGNANIVIMGQPGVGKTVALAHLASL
                     AANRSAKLETLQNAVPFLYHVANLKLPLPDPKKDVLAPLIEAASENAPLMDLNRLPGF
                     FEGAFKSGEALLLIDGFDEITAEYQQLVTDYFKAIMQAYPKTRIVTTGAPEYLDGLIP
                     LGFAPLALMTWSQRENQKFIEHWGELWSQTVAMEAWAQTGPEQVDPLLLNTWLGTDNL
                     NLSPLELTLKAWGAYAGDSLGPHVLEAIATHIRRIAPANTPLAALETLAMQAMVNTQP
                     IFDPRVARAWVKSFEVAEETPTGEATEQAAAGEETEEKAEDTKQLKGKKVEKVSTPTS
                     GLLGKMAASGLLVVYPNNKMRFTHSVFAGFLAGRALMSFKADETILNQADWSGKYLVM
                     RYFAAHGDASKLVQSLMEFSRLPMHRPLFAAARWLRDAPRNAPWRGKLFGTLAAIMQS
                     EGLPLNLRGQAMAAFVVSNDSSITTLFRQLAGTLSFELVHLAVLGAGAMRDAKALEVL
                     ERALQAPSTAVRRAACMALVSVGTNDALETVAHTLLSGEEDIRRAAAEALANDPHEGY
                     AMLRDGVTIDDILLRRAAAYGLSRVSEEWAIELLKKIQIDDKEWVVRNAATEVLDSKT
                     SAGSLAPHKLKAPSESPWLIEFAAKQGMGISPGVPATDILLLALKSDDAETRLAALPY
                     LKYTPNEGVISQLYAAMYKDDHELREAAYNTLWELGASGVKLPDPSQYGFG"
     gene            33603..34955
                     /gene="ssnA"
                     /locus_tag="PLE39_05300"
     CDS             33603..34955
                     /gene="ssnA"
                     /locus_tag="PLE39_05300"
                     /inference="COORDINATES: protein motif:HMM:NF005540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HNE67775.1"
                     /translation="MLITNAKIITWETTNQVLENHAILIEGDRIKEIGTTKALTAKYP
                     KAKTLDARGQYVMPGSICAHTHFYGAFARGMAIPGPAPKDFPEILQKLWWPLDRSLDA
                     EAVRFSALVCLVDAIKHGTTTLIDHHASPNAIDGSLDIIADAVDKSGLRGVLCYEVTD
                     RDGSEKANAGINENIRFLKRLASNPHPRLAGTFGLHASLTLSGITLQACRAAVPEGTG
                     FHVHTAEHESDEYDSLGKSQMRVIDRLQKHGILGPNTITAHGIHFDAREMEILKETGT
                     WLTHQPRSNMNNGVGVAPVESMLRLGVKVCLGNDGFSNAMWEEWKAAYLLHKIHHRDP
                     RRMGGYDVTQMAVYNNAALANQFFPAAPLGQITQGAFADLIFVDYHPYTPLTAGNLPW
                     QIIFGFQQSMVTTTIAAGKVLMKDRELLTLDEAEIAARAREIAPRIWKRYEEEVAKVM
                     "
     gene            34992..35672
                     /gene="npdG"
                     /locus_tag="PLE39_05305"
     CDS             34992..35672
                     /gene="npdG"
                     /locus_tag="PLE39_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HNE67776.1"
                     /translation="MNDSNLLLSIAVLGGTGKEGKGLAFRWARAGYHVHIGSRSAEKA
                     IAAANEIREMLGDGISISGMSNPEAARDSNIIVLTVPYSAHRDTLESVKEELKGKLLV
                     DVTVPLVPPKVATVQMPAAGSAAQEAKQIVGEEVQVCAAFQNISHEHLLDNSEVECDV
                     LVTGTSKEARAETIKLVEAAGLIGWDAGPLENSVVVEGLTSVLIGINKKYGSTHAGIK
                     ITGAKRNT"
     gene            35727..36494
                     /gene="cofE"
                     /locus_tag="PLE39_05310"
     CDS             35727..36494
                     /gene="cofE"
                     /locus_tag="PLE39_05310"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNE67777.1"
                     /translation="MSLSITPLQNIPLIRQGDNLADILVASIQNSTIQLENDDILVLA
                     QKIVSKSEGRMVNLATVTPSPQAIELAQKTEKDPRVVQLMLQESNEIVRTRIGAIIVE
                     HKLGFVCANAGIDHSNVAGDGSENEEYVLLLPEDPDASAKALRDQIKQKTGLNVGVMI
                     IDSHGRAWRNGTVGICIGLSGIPAMIDERGWKDLFGYTLKITVVGVADELAAAASLMM
                     GQASEGIPAVHVRGFPYPLGEGSLKELIRPKEQDMFR"
     gene            36550..36942
                     /locus_tag="PLE39_05315"
     CDS             36550..36942
                     /locus_tag="PLE39_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HNE67778.1"
                     /translation="MKNFPPEYLDLLKDETRAYLFLATTMSDGSPQVTPVWFNMDDEY
                     ILINTNEGRTKDRNMKARPKVAFVIQDPETPYRYIQVRGEVAEFTTQGADEHINQLSL
                     KYDDRPWKYREGQKRIIFKIRPTHFDRH"
     gene            36929..37558
                     /locus_tag="PLE39_05320"
     CDS             36929..37558
                     /locus_tag="PLE39_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNE67779.1"
                     /translation="MTATDLHTFLRTRRSIRRFKTDPVPDTVIESILSTATFSPSAHN
                     RQPWRFAVVTASAVKEKLADAMAVEFQRDLEADAISPENVQRKVSRSRERIVAAPVVI
                     ILSVDMTDMDTYPDTRRKKAEYIMATQSTANAGMQMLLAAHAEGLGSVWVCSPLFAQE
                     TVQNVLGLPRSWEPQAMYFLGYPSETPEVRERKSIKEIVKRVDVQKSTP"
     gene            37569..>37796
                     /locus_tag="PLE39_05325"
     CDS             37569..>37796
                     /locus_tag="PLE39_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HNE67780.1"
                     /translation="MKITALAGGVGGAKLAHGLAQILPSQDLTIIVNTGDDFEHLGLS
                     ICPDLDTVCYTLAGLANPETGWGRVNETWNTI"
BASE COUNT         9431 a        10187 c         9054 g         8924 t
ORIGIN      
        1 cactttacag gatttggcct gtatgaccaa aaagtaattg atattcttaa aggtttgcaa
       61 gatccatatc cttacttccg tggcttgatt gctgatatcg ggtttgagcc cgctatcatt
      121 gaattcacac aacccgcgcg gaaattcggt aaaagtaaaa gcaatctttt ttatctttac
      181 gaagaagcca tgctgggcct taccagttac accaaacttc ccctaaggct cgccaccatc
      241 atcggatttt ccagtgcctt gatcagtttc cttatcggca tgttctacct ggtctacaaa
      301 ctactctttt ggagcagttt ttcagtcggt actgcaccaa tcaccattgg gcttttcttt
      361 ttcgcctcaa tccagcttat ctttctaggt atcattggtg aatatatctc catgatctat
      421 attcacgtgc tgaatcggcc gctggtctac gaaaaggaac ggatcaactt ttgaatcaac
      481 agagcactcc tgtacccttg cctgaagatc tagtggatga aaagccgaaa cgcaccagcc
      541 gcctcatgct tctggtctcg gtcattctgg caggggtatt tttatatttc gccctgcgca
      601 atctcgactg gcaggtattt tttgcagccc tgaaaggcgc caggtactcc taccttccga
      661 tcatcctctt cttgatcaac atcaattacc tttttcgtgc catgcgttgg cgtggactat
      721 tattgaacga aaagcacatt tcagttttca ctgttttttg ggcaaacatg actggatatc
      781 tcggcaataa tgtattgccc gcacgcgccg gggaagtgat gcgtgccgca tacatcgcgc
      841 gcaaggaaaa tatttccatc agttttatca ttgcaacagg agtcactgaa aggctggtgg
      901 atctggctgc cctggtcatt gtcggcgctg tctccctttt ttttgtagac gcacttccca
      961 ggtccgtgca ggatgcgttg aagagttttg caatcatcgc gcttgcaggc gtcgttttcc
     1021 tgttcctcct gccttatttc cacggaatat tcagccgcat ccttgcttcg ctttcatttc
     1081 tgggcgattc actcaaaaca aagatacagg aatttacgtc acgattcatt gaagggataa
     1141 aagtgatttc gcgggcagaa cgtggtctgc catttatctt cttcaccgcc ctgatctggc
     1201 tgctggatgg aatcggtaca gtcttttttg cactggcatt taatgaatct ttgaccctga
     1261 tgcaggcctt tttattcatt gccgcgcttg gactatcaag tgcgatccca tccacaccag
     1321 ggtatgtggg ggtttatcaa tttgtagcgg tgacggtgct tgcgccgttc ggcatcaagc
     1381 gtgaatctgc ccttgccatg atccttgttt cgcaggcgct gaatctattg atcgtgtctc
     1441 tgtggggcgg actcggcctt tggtttggat cacaaggcat ggtcaagtca actcctcaag
     1501 ggaattaaac atggactcga atttcttcct tcaagacata gtcggtatct cgcttgcatt
     1561 tttattattt tcttttgtct ttatatttcc cgggtatgtc atcggctggg gattcaacct
     1621 gttgaagcat agaagtcaac cgctcatgtt gcagcttggt acagggattt ttatctcaat
     1681 ctcggttatt cctgcctttc tatttctggt ctatagattc ctacctgcaa agatcggcat
     1741 tggtatcctt tttgcatttg ctattgctgc tatatatatt tactttaccc aaaacaaagc
     1801 cagtggcaac aatgcggtgc ctgcatcaga aaaaggatat caaaaatccg ttcttggtct
     1861 gattttggta tggtttctgt tttcggtatt tgtttctgcg gatcttcaaa ttggaaaacg
     1921 cctctacttc agcaacaact cttacgatat gctgacacgg gtggccgtgg tggatgcaat
     1981 gacccgtacc ggcgttccgc ctgtaaatcc gtcttattac cctggcgaag aagtgcgtct
     2041 gaatttcctc tattactact ggtatattct cgcgagtgtt gtggacatgc tcggcggccc
     2101 gctggtcagc gccctgcatg ccatgattgc aagcatcagt tggtgtggaa ttgcactctt
     2161 tgcaactgtg tcaacctata tgcaagtccg tgcgccttct gaaaacgcat ggaaaaaaac
     2221 ctttatcggg atacagttgt tcgcagtcag cggattggac tgcatcatca tcatccttgc
     2281 ggcggtaagg gaagggtatc tgcctgtcaa cggcggcatc gaaggctgga atatgccgat
     2341 tatgtcatgg ctcaatgcaa taacctgggt gccgcatcat gttgcagccg caattgcatg
     2401 tatctttgca ttattacttc tcctgcatga aaagaacgaa cgcctacccg ccaagcttct
     2461 tgtttcagtc atgatcgggt tggcttttgc ctccgctttc gggttatcgg tctgggtaat
     2521 gtttgtattt gcgttcttct gggcagtttg ggcgatcatt tcacttttgg aaaagaaata
     2581 tgccagtttg ggagtaatgg tattaagtgg tttttttgcc ttgatcttta tctcaccttt
     2641 tctggctggg cttgtgtcgc caactggtga aacatcaaca gccagtagcg cgtcaccagt
     2701 ggctttttac atccgtccct tcatggcaag cgtcgttttc cccgaaggca ctccctccat
     2761 tgttttggga accgccaact ttttcatgct tccgttgaac tatctgttcg agctgggctt
     2821 tttctttctc atggctgcta tctggattca agagttttac agcaggaata aaaacacaaa
     2881 ccccatctac aaagcggagc tggtccttgc ggctgtaaca acggtggtgc tttccttctt
     2941 atactccacc ctaatctcga tcaacgattt aggcatccgt ggttggctgc ctctccaaat
     3001 cattcttatt gtctgggctg ttgatgtaat taccatgttc atgggaaatg aaaaatggat
     3061 tacacccagg atattccgtg tgttccaaaa cccaaaaagc cttggtctgg ttctgggggt
     3121 tacactgacc ctcggcctgc tcaccacaag cctggaagcc atttcacttc gcacctgggc
     3181 catactcatt gatatgaacg tgaccgactt ccccaactcc atcagcgtgg ataaccatct
     3241 cggcgaaaga acttattacg cccgtcttgc ttactcttac attagcgaaa acactccaaa
     3301 caatatcatc atccagagta atccacaaaa tcaaatggag cgccccagcg gtttgtatgc
     3361 cacccggcag atggtcattt ctgatcgtac aatctacggg gtgccaattc ccgtttacgc
     3421 aaaactctca caagatattg gcagtatttt cagaagaaaa acctcttcat gggatgacat
     3481 tgatacaaaa tgtactcaat acaagataga catacttgtc ttcaaagaca cagacagagt
     3541 ctgggaaagc ctgcctgatc tgaagaccat ccgcccgccc ttgtacgaaa acccgtatta
     3601 cgccttgtac tcgtgtggaa aataaaccta acttttcgta aaccgcatcc gccactcaaa
     3661 cagtttattc aatgcctcaa ttggcgaaag tgaattcaca tccaattcct tcaactcatc
     3721 caaaagcgga ttcgtctcag gaaaaagcgc cacttgttgc gcggcattgg ggtcaatttt
     3781 cacagcgcgg cctgatgtct tttcaagctc agccatgatc tctgtggcac gttggatcac
     3841 tggtgcaggc aaccctgcaa gctgtccaac gtggattcca tacgaacggt ctgcaccgcc
     3901 tgcgatgatc ttgtgcagga acacaacctt gttgtcagcc tcacttactg ccacgttgta
     3961 gttgcgaatc ccgggcagca ggtcggcgag ttgggtcaac tcatgataat gtgtagcaaa
     4021 cagggttttc gcacgcaatt gcggatgatt gtgaatgtac tcaataatgc cccacgcaat
     4081 ggaaagccca tcataggttg aagtgccacg gcctatctca tcaagaatca acaagctgcg
     4141 cggcgtggcg tgatgcagaa tatttgccgc ttccaccatc tccaccataa atgtagactg
     4201 ccccgcatga atttcatcct gtgcgccgat gcgggtgaag attcgatcca ccaacccgat
     4261 ctttgcagat gcggcaggca cgaacgaacc catttgcgcc atcaacacga tcaacgcggt
     4321 ctgccgcaaa taggttgatt tacccgacat gttcggcccc gtgatcaccc gcaccacttc
     4381 acctttttcg aagatgacat cattggggat gtacctgtct gatttcaaca actgttccac
     4441 aacaggatgc ctcccctcat ggatctccaa tccgcttcct tcgtggacct cgggtctggt
     4501 gtagcctccg agagcagcag cctcccccaa agcagacagc acatccaatt cggcgattgc
     4561 ccgggcagtg gcaaggattt gattcgcggc cttcaccaat tcagcacaca cctcacggaa
     4621 caaacgcgtt tcgatctcct tgatccgttc ctcggcgttt agaaccagcg tttcgtactc
     4681 cttcatttca ggtgtgataa accgttcagc attgaccagt gtctgtttgc ggatgtaact
     4741 ctcaggggcc ttgtccgctg cgccgcgtga aatctcgatg taatagccaa acactttgtt
     4801 gtagccgacc ttgagggttt tgatgcctgt cttttcgcgc tccacacctt cgaggttggc
     4861 gatccaatca cgggcgtgct ttgaagcgtc tatgacgcca tccaattcag aagaatagcc
     4921 agggcggatc acacccgtat tttgcagcgt agcaggcggg tcatcgtcaa tggcgttttg
     4981 cagcagagaa agctcctggg tacacacaga ccattgatcg ctgaccactg ctttttgtcc
     5041 gctgatgact tcttttatct ttggcaactc ggtcagagtg gtgcgcatcg caaccagatc
     5101 acgcggttga gcctgccctg ccaacacacg gttgaccagc cgctccaggt ctgcaagtgg
     5161 ctttagtgtc gcccgcaatt ccgcacggac catgccattt tccacaaaat actgcacgcc
     5221 gttctgccgt tgtgtgatct tcgccacatg cagcaaaggc tgactcaccc actgatgcat
     5281 gagacgcttt cccatcggcg tcaccgagaa atccagcgta cccagcaaag accctttgcg
     5341 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcgccgcgc aaggtttcat
     5461 ccaattccaa atttctgcgc gtggaggcat ccaacgtcat gaattcattc aggctgtagg
     5521 cacgcagcga agtaagcaaa ttcaatgcat cgggctgggt ttctttgagg tattgcacaa
     5581 tggcacccgc cgcccttgcg gagaggctgt tatctttcaa cccgaatcca gccaaggtgg
     5641 aggccttgaa atgtgagatc aaaacctcgc tgcatttgcc tggctcgaac ttccatgcag
     5701 gcagggcggt gatgtgacct gtgatcccat caggtaaaac ttgcgaatcg gggtgaatga
     5761 tctcagcagg gtgaagacgg gtcaactcgg cacgcagggc ttcaagcggc aattcggtaa
     5821 cggcgaattc tccagtggtg atatctacat aggaaacaga agcagtgatc gggttggaat
     5881 tgaccgctgt tgtcgtgtcg agaatgaccg aggcaaggta attattcgca tcgccgggca
     5941 gcaatcctgg ttcggtcacc gttcctggtg taaccacacg cacaaccttg cgcggaaaaa
     6001 tccccttgac gggttgttcg ccgacctgct cacagatggc aacgtgataa cctttttcga
     6061 tgagacgtgc cagataattt tcaaccgcat ggtatggaat gccagccaac ggcacacgca
     6121 cgccgccgcc cacggggcgc gaggtgagaa cgatatcgag ttcgcgggct gtgatctcgg
     6181 catcttcatc gaaagtctcg taaaaatcgc cgaggcgaaa gaaaacgatt cggtcggggt
     6241 gctcgcgttt gatctcaaga tattgctggc gtacgggggt caggtcgtcg ttactgggca
     6301 tgggcgctgc tctgaattaa gtttaatgtg tgatttgact tattattata actggagtat
     6361 aagcctttat tccaacccta aatacaaccc atccatatct caaaacggcc catgttcaat
     6421 caccgacttc caaaactaaa cgatgtactg tctgtgtacg cggtcattgc aaccatgctc
     6481 ttcgcgtggt cggctttgct gttcttttgg tacctgccat cctggatgca ttttatgttg
     6541 ctcggcgaaa ttgcggccac tttttcgtac gtggtcattg caagtttttt ggagggatta
     6601 agctttctgc tctttttgct ggtgctgtgt ttttttctgc cgcccgaata tctgcgtgat
     6661 gaattcgccg cgagagggac agccctcacc ctgcccatca tcggcatgat catgatctac
     6721 ttccgcatta cgaatgagaa cattgcgcgc atcatctttt cggttacagg gttggtcatt
     6781 tcgctggtgg tcatactcct gttttatttt tcagcgaaca acccgcggat aaaatcagct
     6841 tttgcggcgc tggctgagcg gtttacggtt tttctataca tcttgattcc gatctttgtt
     6901 ttatcgctgc tgggaatcgc cttgcgaaat atcttctaag agggaaagat gaaaagttta
     6961 agcagacgtg actttttgaa aactgccgcg gccttttcgg ctggctcgct gctatccatg
     7021 gctcctgcgc ttcaaaacat caaagcgcaa agcaacaagc cgaatatcat cgtcatggtc
     7081 tttgatgcca tgtcagcgcg caacctgtcg gtgtatggtt acccgcgcag caccaccccc
     7141 gggcttgagc ggatcgccga acgggcaacg gtctaccact cgcattattc ggcagggaat
     7201 ttcaccacgg ctggcacggc ttccatgtta actggcttgt atccatggac acatcgcgga
     7261 atcaatttga gcggcatggt caacaagaat cacgttgatc acaatatttt caacctcatc
     7321 ggggatgaat atcatcgcac tgcattcaca caaaattatc ttgccgatgt tctgcttgga
     7381 caattccaat caggtctgga tcgtcacatc cctgcgccaa ctttttacga ccaattggat
     7441 catccgttga tcgggcaata ctttccccga gacccgatcc ctgcttatta cgggctggat
     7501 gattaccttt tttctggcgg cattgaccgt gtgggcagca ttccttccgc gcccattttt
     7561 ggttttttga acaaattggc gcgcaggaat caggacttca acaaatcact gcctgactat
     7621 ccgtatggga ttccaaacaa cacgttctat agctacaaac atgaagttgt gtacgcgggc
     7681 gtgctggatg agatcaagaa tctggccagc gaacaaaacc cgtttctggc gtactttcac
     7741 ctgttctcac cacatgagcc gtatcggccc cgtaaacaat tcaccgatat cttccctgag
     7801 ttggaagtcc cattcaaagc gccgacgaaa ttctccaccc tggatattga caacgaccat
     7861 atcctctggc gccgcaagac ctacgatgaa ttcgtggcgg atgtggattc tgagatcgaa
     7921 aaactcgtga acgcgctgga cgaatcgggt attctggaaa atacctatct aatcctgacc
     7981 gccgaccacg gagaattatt cgaacgcgga gagtatggtc atgtcacccg attgctgtac
     8041 gatgcagtgg tgcatattcc gctcatcgtc attgccccag gacaaaaatc tcgtgtggac
     8101 gtccaccacc ctaccagcaa cattgacctg ctgccgacac tgctgcatat cgctggcaaa
     8161 acggccgcaa ctgacctcga aggcagaatc ctgccagggc tgggcggaat cgaagagtca
     8221 cgcagtttgt tttcgatgga agccaaggaa tgttccgcat ttggaaatct caaagatggt
     8281 gctactgttt cgctgatcca aggcgagcac aaactgatct actacactgg ctacccgaaa
     8341 cgcccagaca cattcgaact ctacaacctg aaagatgacc ccgatgagat gaaagacctg
     8401 ttcacggttg acaccaccac tgctgcaaaa atgaaggaag aaatgctgga tacttttgaa
     8461 tccaacagga aattaaaata gtctaaacct ctcaaatgga caagggttgt gatgtcagat
     8521 ataatcttca accgaaaaaa ggagtgtctc aaatgtatat tgacccaaac acaggcggca
     8581 tcttattcca ggctcttgcc gcagtattcg tagcaggctc gggcgtattg ttcttcttct
     8641 cccgcaatat tcgtgaaggt atcgcaaagc tccgccgccg tttccgcaag gaagacgaag
     8701 agcaaaatca gtaattcggc tgcatgaaga cgatcattct tggctttgac tcttttgacc
     8761 ccgctgtgtt cgaagacatg gcggggcaaa ataaattacc gaacttttcg aaattcatgg
     8821 acgccggggg gtattcacgt cttgaagtat gttcaccacc acaaaccgaa gtttcatgga
     8881 cgagcattgc cacgggggca gaccccgggg ggcacggcat ttttgatttc gtgcatcgtg
     8941 acccagccac atacgccccg tatgtttcga tcctgccgat gaaaaaaagc gcgatgggag
     9001 agcaatttgt ccctccatac atcgcgaaga ccctttttga agaagccgcc agcatgggct
     9061 acccagccac tgctttatgg tggcctgcca tgttcccagc acggcccgaa ttgcccgtca
     9121 acacactgcc cggcctcggc actcccgata tccgcggaca gttgggagtc ggcacgcttt
     9181 tcaccaccga ggatgaaaag aaaagcaaga ctgcggtaac gcgtttcgaa tccacgggca
     9241 agggcaggta ctctgctctg ttgaatggac cgcaagtgca aggcaagaac ggtccgcagc
     9301 ctgccacgtt gccgatccag cttgaagcgc tggaccagac ttcggcacgg gtaacgatcg
     9361 gcgatcagca gatccaactc cgcgttggtg agtggagcga tatcgtggaa gttaaattca
     9421 aagcggggtt attcctgagc attcacgcca tcacccgcat gatcctcacc agcttgaacg
     9481 gtgttgtgcg tttgtacacc ctgccgctgc aaattcatcc actgcacgcg ttatctcatt
     9541 attcttcttc gaaatcattt gcgaaagacc tgtggcagaa agtggggccg tatctcactt
     9601 tgggttggcc gcaggacact actggtcttg aagatggctg catcaccgat gaacagttca
     9661 ttgccttgtg cgacctgatc ttcgagcgcc gcaaacaaat tttcttccat ttgcttggca
     9721 acttcaaaga gggcgtgctt ggcagcatct tcgatgacct agaccgtgtt cagcacatgt
     9781 tctttcataa ccgtctcgac gtggcgcagg cctggtacca aaaactggat gcgtttgtcg
     9841 gcgaagtcag tcaacgcgtg gaaaaatggg gcggtaaata caactacctc atcatgtctg
     9901 accatggctt cacgacctac cagcaaaaga ttcatctcaa tcgttggctg gctgaaaatg
     9961 ggtacctcgc cacaagcaat ggcagcgccg aaggcgacct atccaacatg gactggcaaa
    10021 agaccaaagc ctacgcagtc ggcttgaaca gcatctactt gaatgtggct gggcgcgagg
    10081 gcaaaggcgt ggtcggcgcg aacgagatcg aatccctgct ggctgaaatc caacagaaat
    10141 tgatgggttg gaatggcccc gatggaagac ccgtcatgca gcgtgtgcgg ttcaaacacg
    10201 aagtgtacaa cggagcctac acccgcctcg gccctgacct ggtcattggg tacgcacctg
    10261 ggtatcgcgc ctcctccgaa acaggactcg gcaaagtgcc gcaaaattta gtcgaagcca
    10321 atcacgatca ttggggcgcc gaccactgtg tggattcgga tgtcgtccct ggtgtgatct
    10381 tcgccaaccg cgacctgcaa aactttggcg gcgtttcctt ccgcgatatt cccttcctcg
    10441 ccattggtaa acacctcgac caatcacaca tcaaaccgcc gtcgcaagtc agcggtggca
    10501 gccaaaaaga cctggaagac cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnattcaggt aatcctttga aaaatctatt ctcacgccaa acaccgaaaa agaccaatga
    10681 aaagatcgtc gtggtttctg gtctgccccg ctcgggcaca tccatgatga tgaaaatgct
    10741 cgctgaaggc gggttgccca ccgttgtgga ttcacttcgt caggccgatg aagacaaccc
    10801 caatggttac tttgagatcg agcaggtcaa ggcgctcaaa gacggcgaaa agaactggat
    10861 ctatgatgcg cgcggcaagg ttgtcaaagt gatttcgtat ttgcttgaat acctgccgaa
    10921 cgacctggaa ttcaacgtaa tcttcatgga cagggaaatc cacgaagtgc tggcttctca
    10981 aaagaagatg cttgaacaca gaaatgaagt ttccgccatt tcagatgaag atatggaaaa
    11041 gcaatatcgc gagcatttga aagccgtccg ttactggctt ccacgccagc ccaatatgcg
    11101 cgtgctgtac gtgaaatacg accaaatggt caaatcgccg caaacgcttg tgccatccat
    11161 cattgaattt cttgagcgcc cactcaacgc agaagcaatg gcagctgtcc cgagcccaac
    11221 cctctaccgc aaccgcaaac cataacctgc aatgcccgac ctgtaacatg caatcttaaa
    11281 ttcatggagt ccatcatgta taaacttgtt ctcgtccgtc acggacaaag cacctggaat
    11341 ctcgaaaacc gcttcactgg ctggaccgat gttgacctta ccgaccttgg ccgctccgaa
    11401 gcccgcgaag caggcaaact tctgcgtgaa ggcggctatg atttcgatat cgcctacacc
    11461 tccgtattga aacgcgccat caaaacactc ggcctcgttc aggaagaaat ggaccgcgag
    11521 tggctgcccg tggttcgcgc ctggcaattg aacgaacgtc actatggttc tttgcagggc
    11581 ttgaacaaat ctgagatggc tgaaaaattc ggcgaggagc aggtcaagat ctggcgacgt
    11641 tcctacgacg tgccaccccc agccctggaa ctgaccgacg aacgccaccc gaaatttgac
    11701 cgccgctacg cttctttgac tcccgaacaa ctgccagcca ccgagtcgct taagatcacc
    11761 cttgaccgtg ttctcccctt ctggcattcc acgcttgcgc ctgagatcaa atcgggcaaa
    11821 cgtgtgctga tcgtggcgca cggcaattcc atccgcgcga tggtgaagta cctcgataac
    11881 atctcagaag ccgagatcac cgaactcaac attcccactg gcctgccgct cgtctacgag
    11941 ttggatgaag ccctcaaacc catcaagcac tactatctcg gcgatgcaga agaagccgca
    12001 aagaaagccg ccgcagtggc aaatcagggc aaagccaagt aaaacccgtt ttaaaactaa
    12061 aaatcaaagc aggcatggat ggagcacaac acctgtccat gcctttttta tccagtcagc
    12121 gagagttagc ccatgaaact actgaagctc atttctgaac gaatgccaga ctcgaaaaac
    12181 attttggcgg tgtatgcctt catcgtcatg ttggtctact cgtggaccct gttcacatcg
    12241 ttttacaaac tgccttcgtg gctgttctac ctttccattg ggcaaattct ttcggtgtac
    12301 gcctacgcgt tttcttttga ccttctcgaa agcctgattg cattgacggt catcctgctt
    12361 ttggattaca cggttttcct cttcttgaaa aacaaggaag agtttcagtc gcggtccatg
    12421 attctggcgg gtattttgct gggcacatcc atgacgcgac tggcgttatt ccaaaattat
    12481 gaagatatca ccgccttttt gagcggcgaa ttgatctggt gggccattgc cattccgctg
    12541 gggttggtcc tcgctgcctt cgcgccgaga atcaaattgg tcaggaccat ttttgaggga
    12601 ttcgcagagc gcgccacggt cttcgtctac atttacattc cgctgagtct gatctcactg
    12661 gttgtggttt tgatccgcaa catcaactag gtgaaccgat gacaaacttt tcccgcaggg
    12721 atttcctcaa actcctcgga gcggcatcgg tcggcgcgct tgcctctgaa cgcgtgcttt
    12781 cgctcaaagc cgccgacaac agcaagccga acatcatcat cattttatgg gatgcgtttt
    12841 ctgcaagaca tttatcgttg tacggctatc cacgccagac cactcccaac attgacgcct
    12901 ttgccgaata ttccaccgta tatcacaacc actacagcgg cggaaacttc accacctgcg
    12961 gcactgcctc catgttgaca ggcatgatcc cctggaagca tcgcgccatc aactacgatg
    13021 ggctggtacg tgcagagtac atccacaaca atccatacac cctgcttggt tccgattaca
    13081 agcgattgat gtttgcacaa aacccctggc ccgatcgttt ggtgggacag tattacgaag
    13141 atgtagaccg cttccttccg cccaccgctt tcagcctgct cgaggattcg cccgtctttt
    13201 ttgaaaatga ccgcgccatt gcatctgtgg cgatgagcag ttttctgatg ccgatccaag
    13261 gcgggaacgc ccccgtgggt tcatccattc tgggttacat gaacaaaagc agagttttga
    13321 acttcgctga tgggcaaaaa tctccacgct acccgaaggg cattcccgaa gcgatgaata
    13381 tgggctacat gatcccctat ctcaatgagg atgtgtatga agggttgttc tcagaaatga
    13441 cccagcttga aaagaatggc gacccttttt tctcatactt tcatttgtat tcgccgcact
    13501 acccctaccg tccgcgcaat gattatttga agatgttccg tgatgacggt tttgcccctg
    13561 tctcaaaacc gattcatccg ctatcggtcg gcctgcagca ggattacctc aacggccaaa
    13621 gggacatgta tgacaggcag atcgcccaga tcgatgaaga attcggcagg ctgattccac
    13681 gccttgagga aagcggcgtg cttgagaaca gttacatcat ttttaccgcc gaccacggtg
    13741 aattgttcga aagaggcttt gcagggcatg gattccaatt catgtacgag tcggtcctgc
    13801 atattccgct catcatccac gccccagggc agacaaaacg gcaggatatt cacgccctca
    13861 ccagcaacac agacatcctt ccgaccatcc tgtctattgc aggcaaggaa accacaccag
    13921 acatggatgg gcgcatcctg cctgggctgg gcggcgaagt ggacgaagac cgccccatca
    13981 tctccctcgt cggcgtggac aactccgcat ttggtgtcat caaaaaggcc gtcatcgcaa
    14041 tgcggaaacg cgaattcaaa ctgatcgcat acctgggcta cgaagacagt tacaaaaagt
    14101 catttgagct gtacaacatt gaggccgacc ccgaagagct cgacgacctc tcagaccgcg
    14161 ataccgccac cctttctgcc atgaaggatg aattattgac ctatctcgac gaagccaata
    14221 aaccgttcag gaagagctag caattccatg gcgcagatca cgagaagaga ctttctaaaa
    14281 ttggcgggcg gtgcgctggc ggggtcgctg gtttccagta aattgacttc gcttgcacag
    14341 ggcgacaaag actctcccaa catcatcatc attctttgtg atgccttatc cgcacggcac
    14401 atgtcggtgt acgggtatcc gcgtgaaacg acaccgtaca tcagcgcctt tgccgaccgc
    14461 gccacggtct tccatcaaca catcagcggc ggaaatttca ccagcacagg cacagcctca
    14521 ctgttgacgg gcatgtttgg ttggaagcat cgcgccatca atcaaggcgg attggtgctg
    14581 cccgaatacg caaaaacgaa tccctattct cttctcagca cagattattt caaattcgct
    14641 ttctcccaaa acccgtggtc agaccgtttg atcgggcaga acagccgcgc cgtggataaa
    14701 ttcctgcctg tcacctcata cagtctgcgc gaggaaaatc cgatctcgcg aattttcaaa
    14761 aatgaccgca cactggcctc gatcgccctg gatgacttta tgatcccagt gcagggcgac
    14821 accccaggct catccatcat cagtgcctac tataaaaaga aagcaatgga cactttcttg
    14881 aaagacaaga acaagtccgc acgctaccct gatggacttc ctgaaattct catcggcgga
    14941 tatgtggcgc catatttgaa cgagagtgtg tacaacggcg tgtacgaaga agtctcacaa
    15001 cttgaagcga gttcacaacc ttattttgcc tacttccatt tattctcgcc gcattttccg
    15061 tacagcccgc gcagagaata tctgaaatta ttccgcgatg atttttcgcc gccgctcaaa
    15121 cccattcatc cactttcacc caggctgcac aaagatttta ttgcttcacg cagaaccgcc
    15181 tacgaccgac aggtggcgca gatcgatgag gaattcggca ggctgatcgc caaactcgac
    15241 aagctcgaag ccctcgccaa cagttacctg atcttcaccg ccgatcatgg cgaattgttc
    15301 gagcgcggct tttcagggca cgggtttcaa tttatgtacg aaggcgctct gcacacgccc
    15361 ttattgatcc acgcgcctgg gcaagcctcc cgcgtagaca tccacacccc caccagcgca
    15421 gtagatttgc tgcccaccct gctctgcatc acaggcaaag acatcccaac agatatcgat
    15481 ggcaaggttc tgccaacgtt tggcggtgat gaagacaccg agcgccccat cttctccgtg
    15541 tatgcggtgg acaactcggc atttggtccg ctgaaaaaat gtgtcatcgc catgagaaag
    15601 ggcgcacaca aactcatcgc gtatctgggt tacaaaaatt acgatgaggt cttcgagctg
    15661 tacaacatcg aaaccgaccc cgaagaattg ttcgacctcg ccgccaaaga attggacctg
    15721 ctttcgaagt tgaagcagga attcaaggcc cacctcgaag aggcgaacaa accgtttgct
    15781 aaaacataag tcgtttcatc aaaatagaag caaagtgaac gttgcatcac gaaatcaagt
    15841 ctgcgataga atcaccgcat gaccctctcc cctgattttc ttcatgaact aaaaaagcgc
    15901 ttcactggcg acctgcggct tgatcctgcg tcacgaacct tatattccac cgacgcatcc
    15961 atgtaccaga tcgagccgtt ggggattgca attcccaaaa cgcaggacga tctacaagcc
    16021 gctgttgagc tcgcggcgaa atatgccatt cccattttgc cgcgcggtgc agggtcatcc
    16081 ctcggcgggc aggccatcgg cgaagccctg atcctggatt gctcacgcca tctggattcc
    16141 atcctcgaaa ttgaccctga ctctcaaact gcgactgtgg aaccaggcgt cgttctttcc
    16201 acgctgaatg ctgcctctgc caaatttggc ctgaccttcg gccccgaccc tgcttctgcc
    16261 gaacgcgcca cgatgggcgg ggtcattggc aataacgcca cgggggcgca ttccattctg
    16321 tacggcatgt ctgcagacca tttggtttca gcggacgtga tcatggcaga tgggagtctt
    16381 gcagtttgga acaaaatcgt tttggaggaa agggaaaaga ggaaagacga ttcacttttt
    16441 tcctctttcc tctccacagc acttgatatt cgcgaaaaat actcaaaggc tatcaaacaa
    16501 aacttcccca aaacctggcg caattcggcg ggttacaggc tgaattatct tttgccgtgg
    16561 agtccatccg ccccgccgca gtggatcggc gattcatatc cagcgaacct gcaaccttcc
    16621 atcttcaacc ttgcaacact tctcgctggt tccgaaggca cgctggcggt gatgcagcgt
    16681 gccacggtga atctggtcaa aaaaccgaag cacacgatcc tgggagtgct ggcttatcaa
    16741 agcattgccg atgcctgtga tgatgttccg cgcttgctgg aattcaaacc cagcgccatt
    16801 gagttgattc cgcagatgat cattcaactg gctcgcggtg tcccttcatt tgcccgccaa
    16861 atgggttgga tgacgggaaa ccctgctgcg gtgctggtca ttgaatttag cggggatcaa
    16921 ccgtcggctt tgaaagaggc ggttcgtcgt gtcggcaatg tcattacgat cgccgagtcg
    16981 cccgaggaac aggctcgtgt atggaacatc cgcaagatgg gattgggaat tttggattcg
    17041 cgcccgcaag ccgcaaggcc tgccgcattc atcgaagatt gcgcgatccc cgtggaacga
    17101 ttgggcgaat ttgtgcgtga gatcgagcgg attctgcgcg accatcacac cgaaggtgga
    17161 atttacgcgc acgcttcggg cggttgtttg cacattcggc cgattctaaa tctgcatctt
    17221 ggcgagggtg tgcgggcttt gcgaagcatt ggcggacagg ttttgcaatt ggcgctgcga
    17281 cttggcggct cgatgagcag tgaacatggc gatgggatcg caacaggcga gtggatcgaa
    17341 aaaacatacg gtgcagaagt gaccaaagcc atgcgcacct tgaaacacgc cgccgacccc
    17401 agaaatattt tgaacccgaa gaaaatgttc gatgcgccgc ccatggatac aaatttacga
    17461 tatggcgaaa attatcaggc gaagacctgg gttccatctt tgcatctcga tcatgagcgc
    17521 gggctggcag gtgcaatcga gcactgcaac ggtcagggcg tatgcagaaa aaccaacggg
    17581 gtgatgtgtc cctcttttca agcgactcgc gacgaagcca attcaacacg cggcagggca
    17641 aatcttctgc gcgggttaat taccgattac cacttacctt ccgcagaaaa cgcaaggcag
    17701 aaagaactca tgcaggccac ctaccaagcc cttgatcttt gccttgcctg caaaggctgc
    17761 acctccgaat gccccagcgg cgtggatatg ccgaagttga aatacgagtt catgaacgag
    17821 tattacaaaa cccatcgcag acacctgcgt gattatttgt tcggatattt ccatgtggtt
    17881 tcgaaatggc ttgcgcctgc ggcaccgctg gcaaatgcgt tcatgcaaat gagctgggct
    17941 cagaaactga tcgcccgcac aacggggatc acagagaaga ggccgttccc attatttgca
    18001 aagagaagca gcgcacagaa agcatacccc aatgcatgga aaaatggaaa agccgtcatc
    18061 tttttatcgg atgtgttctc gcgctatctt gaacctgaag tggaacaagc tgcctttgaa
    18121 cttttgcatg cagcagggta cgaggtgaaa acactgccca tcatcggctc gggggcatct
    18181 ctgctttcaa agggattcgt tgacgcggcg cgaaaacatg ccgaaaaggt tctcagtgaa
    18241 attcatgcgc ttgatcccga ttcaacgttg agcgtggtcg gatgcgaacc gccagaagtg
    18301 tattgcctca agcatgaata ttcggctttg ctgccaaatc gccgcacgga actcgaatcc
    18361 atttccaggc gggtgtggct ggtggatgag tttttgttac gggatgaaaa cctcgaaaga
    18421 ttggtgcaga tgggggaaac tgccccccac agtgaggtca tccattttca tccgcactgc
    18481 caccaacgcg ctgaagggct ttcagaagac ggcctgccca atggaaccgc cgccaccacg
    18541 gaattgctgc gccgattcgg ctttcaagtg aatctgctgg atacaggctg ctgcggcatg
    18601 gcgggtacct tcggctacga cgcaaaccac tatgagcttt caatgcaggt cggtgaatta
    18661 aagctgtttc caaagatcag ggaattggga tcgggaaatg gagacttggc ttcttctggc
    18721 tcggcttgca gattgcagat caagcaggga gttgggaaga ccgcggagca tccactggtg
    18781 gtgattgcga agcgcatctc tccaaacgga taaagaaaat cccgccctgt agaaatctac
    18841 agggcgggat ttttgtatta tcaatttttt cgtctactct tgcgagatcg tgacgctttc
    18901 catcgcatca ccaataaagc tgggattttg ctggggatcg cgaagcgtga gtgaattgac
    18961 aacatccatg ccgctgacaa cctgaccaaa aatggtgtag ccgccgttca gccattcagc
    19021 agggccaaag gtgatgaaaa actggctgcc atttgtgtca ggaccagcgt tggccatcgc
    19081 caaaatgcca ggcttgtcaa aggtcaggtc gctatattcg ttgacaaact cataaccagg
    19141 tccgcccatg cccgaacctg tggggtcacc gccctgcgcc atgaatcctt ccaaaacacg
    19201 gtgaaaggta aggccgttga aaaatccttt gcaggtgagg aacacaaaac tgttcacagt
    19261 gatcggggct ttatcagcaa acaactcgat cttgaattcg ccgccgttcg ccattttgaa
    19321 ggtggcgaaa tatttggccg tggtatcgat caaaatttca ggtgcggaat tgtattgggc
    19381 tgccgcaggg atcgaatcga acgctgcgca ggcagaggaa gatccgcctg caggtggaac
    19441 aaccgcagga gccgttgctt caacgacggc tggttcagtg gcaagcggtg cagaggtcgc
    19501 tggagcagac ggagagaggg aacaggcgag cgaggcgatc accaaaacag cgaccgcaaa
    19561 ttggatcttg atatttttgt acatgaaaac actccttgag aattgaatta cagggggcta
    19621 ttttatgaag ataatgaaaa tcccgccacc ccaaatgcca cagccacatt gaacgagcgt
    19681 ttctgtccgt gcatggggat gtgaaaaacc gcatcagcaa gctcaagcag ttcggggtca
    19741 accccagtca cttcactgcc aagcagcatc acaacggggg atgggaattg aaactcagca
    19801 taacgcgaaa tgggaatcga gcgctcgtct tcttccagag caacaacttt ccaaccttcc
    19861 aactttagac ctgtaaccaa cttgaccgaa tctttgtgat ggctccaggt cacaaattca
    19921 tctgcgccga gggcggtctt gcgaacggca tccacttcgg gcgtgggggt gattccgcac
    19981 aggtaggcat ggctgaaccc aaatccatcc gcgctgcgca ggatcgaacc aacattccat
    20041 gccgagcgga tgttatccaa caacacagct ttcatttcct tcggtttact tctttcttca
    20101 tccacaacag cctctttttg gatctgtctc cccaacacga cgcgagtctc tcccaggcag
    20161 tgcgggcagc gcaccccaaa ggaatgtcca attgtgattg ggtaacgcaa tccgcaactt
    20221 tggcacactc gaacttcaaa agatacatat gacatgcccc gattatattc gcttgtacac
    20281 caaaccccag cctgataatt tttgactggc aattcagaca acaagcggta aaattaaaag
    20341 atgcagacaa ctagacagct ggtgccagaa tttatctatg ataaatttcg caataacgag
    20401 ataagcgggg tctttcaagg tgccgccatg tttgttgacc tttcgggctt ttcaaccatg
    20461 acagacgccc tttcagcaca cggcacacat ggcgccgaag ttctggcgaa tatgatgcgt
    20521 gtggtcttcg agccgttggt gaatgccgtg tacgaacagg gcggatttgt gatcggatat
    20581 gccggggatg cgttcaatgc tgtttttccc accgagcagg atgcaggtca gaccatgagg
    20641 cgttgtctgg cagcggctgt ccaaatgcag gctcacgcca aagccaatca acttgcatcc
    20701 acaccttatg gagatttctc catccgcatc aaggtgggga ttgggtatgg acaggtgacc
    20761 tggcagatct tcaagtccat caatggaaaa cgggccagtt attggttccg tggtgaaagc
    20821 cttacggggg ctgttcttgg tgaagaacgc gcatacgcag gcgagatcgt agccgacccg
    20881 atctcttaca aactcatcag cgatattgtg gaagttcgac ccgtgcagga atgttttctc
    20941 atcgaaaagg tgattgccga cctgcctgcg gcgcttcctg ttccaacgcc cgagcctgcc
    21001 gacgacctcg caagcatttt cctcgccgac accattgccc acctgcccat ttctggcgag
    21061 tttcgtcagg ccatgaacct tttcattgat attcctgtca acatttcaga tcaggctttg
    21121 atcacgcctt ttatggaaac ggtttatctt ttgcaggaac aatacggcgg cgttttcctg
    21181 cgccccgatc ttggcgacaa aggtttcaac ctgctcatgt tctggggcgc gcccatcgcc
    21241 tacgaaaacg atgtggaccg ggcgttgaat ttcatcatcg aactggccac ccgaactcgc
    21301 atcacattgc gggctggcat cacgtatcac atggcttatg cagggtttat cggttcaagc
    21361 aaacgcgaag attacaccgc ctatggatgg ggcatcaatc tggctgcccg catgatggaa
    21421 catgcaggtc agggcgagta ttggatgtct gaagaagtgg cccgccgtgc tgaaaaacat
    21481 ttcaacctca acttcctggg ggaacatcat ttcaaaggtt tcaagcagga tcaaaaggtc
    21541 tttagcctgc tcggaagaaa aaacattgtt gaaaccgtgt atcagggcca actggttggg
    21601 cgcgaaaaag aaatggaaaa tttgcgctct tttacagaac ccatccatcg tggaaacttt
    21661 gcaggtgtga tggttttgca aggcgaggca ggcatgggca agagccgcct catccattct
    21721 tttcagtttt cagaatattt tactgaacac cccgcccgtt gggtcgtttg ccaggccgat
    21781 gagattctgc gcctgccatt caatccattc aaagactggt tgaaaaagcg ttttgaatta
    21841 ctggaaggcc agcccgatga aatcaacgca attgccttta accgcaacct gcaggagctt
    21901 gccaacatca ccccagaccc cgaactggcc gcggaactca tccgtacccg ttctgtgctt
    21961 tccgccctca ttaacatcac ccagcccgac tccctgtatg aaagccttga tgcaaaaggc
    22021 cgctacgaca acaccctgat cgccttaagt gctttgttgc gcgcagaaag tctgcaaagg
    22081 ccactggtcc tcttcctcga agatacacat tggatggatg aagatacagg caatttcctt
    22141 tcctacttca tgcgcaccct gctggcagaa tctgaaaaac aataccccat cgccatcatc
    22201 accacccaac gccctgaagg caatgcaaat tgggaattgg atggaactta cgctcaccac
    22261 atcaaattag agaaacttcc agccgccagc attgctcatc tggcaaaaaa cattgtagac
    22321 catccgatct cagcatccct gcttgagctt ttgatttccc gcaccgatgg caatcctttt
    22381 tttgccgagc aggttttgcg ctatctcacg gaacataacg cattgagcct gaatgaagaa
    22441 ggggaatata ttgccaatca agccgagaat atctccctgc caaccgatgt acgcgccgtg
    22501 ctggtggcac gcctcgaccg tttaaccaag caggtgaaag aggcagtaca aaccgcttca
    22561 gtgcttggcc gcgagttcga aatccgcgtg ttgggtttga tgctgaaaaa tgataaaagg
    22621 cttctatctc atgtggcaca agccgaaagc gccaacatct ggaccccgct cagcgaaatc
    22681 gaattcatct tccgccatgc cctattgcgg gacgccgcct actccatgca tttggtcgca
    22741 cgtcaacgcg aattgcatgg gctggcagtt tctgccatgg aaacgatcta tcacgaggat
    22801 cttgagccac actacggaga attagcctat cacgcagaaa aagcggccct gaacgagaaa
    22861 gcgcttcact accttaatct ggcagcggat tcagcgtcac aggcatatca aaacgcccag
    22921 gttattgatt atctcacccg cgcccataac ctactgccgc aaaatagcca acatgaacgt
    22981 tttgaagttc tgtacaaacg ctccattgca tattccatta tcggcaatcg aagcagcgag
    23041 gatatggacc tcaccgccct tgaagaaaca gcttctgcac ttagagacaa caccaaactg
    23101 gccagagtat taatgcgacg cacctattat tttgccacgc aaggtgaaaa cttcatggcg
    23161 atctcatttg gtgagcgcgc tgccacactg gcccaatcag tcaatgatat ggagacattg
    23221 ggagaggttt acaacttcct gcccagtgca tttacacgcg tgggaagttt gaaagaggcc
    23281 tttgattgcg ctcagaaggc cctgaagctt tcacaatcca caaacaaccg ccttggggaa
    23341 ggcaatgccc tgacttcgct gggattaatt gcactcgaaa ataagggacc ctccgaggct
    23401 tatcaatatc aagccaacgc tttgaagatc gcccgcgaat tggggaatcg tgacctggaa
    23461 gcaaaagtac ttaataatct ggctaattta atcggcttgt ctaacggtga ctattccacc
    23521 gcccgtgact acttcgagca ggcgcaggta atttaccagg aacagggaag aatgtatcct
    23581 tccgggcttg cgctgaccaa ccttggctgg gtttccaaca tgcttggaga ttatgaaaca
    23641 gccctaacct accatacgcg tgcccttacc attgcacgtg aagtaggaaa taaaacccag
    23701 gaaatgtaca cactcatcaa cctgagcgca tccacatttg gcaagggaaa caatcccaac
    23761 gctgcaatct attgggctga aaaagcgctg tggctttcag aaaaaatcgc cgaccaaata
    23821 ggcaccgcat ggtcattttt ttatcttggc tatgggtaca tgctgggcaa acagtaccct
    23881 gccgcccgtc aggcatttga aagctcgcta aaaatcaggg aaggacttga cgcaaaggta
    23941 ctaacagccg aatcccaagc tgcgattggg caaactctgt acgaattaat ggacattgaa
    24001 gccgccttca tcgaggttga gaaagttttg gaatatatgc aagaaaatcc aaaccttgaa
    24061 ggcgcagagg aacccctacg tgtaatgctc acatgctatc tcgtcttaaa agaaaaaaga
    24121 gacccacgcc ataaaaacat tttgcaaaac gcaaatctgc ttttggatac gcaggtctca
    24181 aaattacgtt cagatgaggc acgtcacgtc tacgtggaaa atgtcccctg gcgacgcatg
    24241 ctcaggaaat attacaagga aacgcagtaa ctcttaaggc caatgaccga tcgcgggatc
    24301 gccgtctgtt aattcaaaac ggttgttctt gatctcacct gtatagtagt agggatttcc
    24361 attcccgtca aaaatgatca actttgaaga gccaatattc ggaatggtga caaaaaatct
    24421 ctgattgatg tactgtccat tctttttgat ggagaagttg ttgatccagg taatggggtt
    24481 accatccacc atgttggctg gcaggccttc gacactgagc ttctcaacat cgtaatcgcc
    24541 atcatccacg atatcaatcg tgatgtctga ttcagagact ttatccttct cgatcttgta
    24601 ttttttatta ttatccttat ccttcttcac cttgcctggc acaacgactt tgttttgcat
    24661 ggggactctc cttgggaatg ggatgatatg tcaaaatcca cctttggggt ggcgtatttc
    24721 aaagacgggc atattatatc gcctgtcatt actaaccaat aaaccaaata agcgttctat
    24781 ggatttttgc cgccagatct caaaatcatg gctgcgccgg aggctccata gagagtcata
    24841 agcgccatcc aggtccaggt ctctgcaatt gcccaattga ccttcttttc aggcggcacc
    24901 actgcgtcca ctagaagcag gccaatgatt gcacaggcac caattaccag cagagtcaga
    24961 ccgagggttt tccattccag tttggaagcc tgacgggtaa tcagaaatat cccaacggcg
    25021 ggcgtaatga aaacaacgct gtacatctgt ccgtgaaatg catcaatggg ccagggccaa
    25081 aacgcagata ctgcggtggg ggcgatcaac agtgcaagtc cgtatagccc gaacaaggct
    25141 gacaacccca aggcataatt ccgccacgtc gcaggggttg gggtggattc tgctggcgcc
    25201 cacgctcgat aaagccacaa atgataggca ctgtttagag gcaaaacaac atacaggaga
    25261 aaccagatcc acggctgcca tctctgcaga tcaaactggt caagatggaa cagcgaaacg
    25321 cccagaacaa tcgttgtgaa aataaaaatc atcggaagga cgatccgtcc gggcgaccat
    25381 cgcccataaa atgccatgaa ccccgctgaa acaaaggaac caagatacac tgtcccgaca
    25441 aaacggccat tgaacggagc aatgacccag ggccactgct cccgtgcaag ggctggcaaa
    25501 aagaaaagtc cgccgcccgt tacgatcagg acgaggactt ccaaccaggt aatgccgcgc
    25561 aacaaagacg aaataggtgg gtttgcagaa tccaactgtg aatttctcat catatgctcc
    25621 aatcaataat ttaggttcta caacagcggc agggtcaacc gcacaattaa ttgggccgct
    25681 gttatccgct tctgggattc atcttatgca aggaatccaa gtacaggtct tccacttttt
    25741 ttctggccca gggggtcttt cgcagaaact tcaagcttga ttttatgctg gggtcattgg
    25801 taaagcactt gatctcgata cgcctgccca attctgccca gccataatat tccgacaatt
    25861 gagtgacgat catttcaagg gtgattccat cagggatgtt tttagggcgg tctgaattca
    25921 ttctacagtc cttttactct gctgcaggca gaggttcaag attaatgagc tgcgagacag
    25981 ttaccagggt atatcccttg ctctgcagat aatcaatcac caaaggcagg gcttcagccg
    26041 tatgccagcc cctcccattt ccatgcataa tgatgatcga accgttttgc actgtccgtt
    26101 gaacctcagc caggatcgtt gccgcatcga aggtcggatc agggtctccc gtgaccgagt
    26161 cccattgcac tgcatacaac ccatgttcag aaacaatctg caaggcagta tcgttataaa
    26221 acccggatgg aaagcgaaac aggtgcgaac gtttccccgt caattggaac atcatatcct
    26281 cggtcatgtc aatttcctgc cccatttgtt ccgcggtcaa ggttgtaaaa tcggcatgac
    26341 tccatgaatg gttacccaat tcaaaattcg ggttggcccc caacaaccgc gtttcgtcag
    26401 gatgggtacg catccaatca ccgcccataa aaaaagtggc agggacgtta taccgctgga
    26461 gaacatccac aatccctgca tcataccctg ccgggtataa gggatcctgg cacatatcga
    26521 aggttaaagc cacgtatggg agagtccagt caccgctggt gacgagagca gcaggcggag
    26581 ttggggttag gtatgcagtt gccgttatcg tagggaccgg gctgggcgtg aaagaagctg
    26641 cgactgtagg tgtgacatca ggagtggacg ttacaagcga ttgaggcgcg gcgcaggaaa
    26701 ctacaaacaa tccgatcacg caaaatacaa tggctttttt tcccatttcc actactccca
    26761 agtccacttg ttgtgaccac gcccagagaa aacttctaag gcgctgggct ggacatttcg
    26821 aagtaatcaa acaaagcagg gatggtggtg ctgatgccat ccacattctg gcctgccaaa
    26881 acgcccactt ggaggggagt catatcactt tcatgcgccc cgactttata aaacaccttg
    26941 ccatcaccgc tgatctcaaa aatataactg gtcgccttgc gggtcaagcg caagactacc
    27001 gcattattct tataagattg ggcaaaattg ggcggcacga tcaatccccg cccgtagtaa
    27061 ttcatgtaga gcccccggct cacacacccg ccgccgcggc aatatccctg cccggcctga
    27121 atgaagttgt tgttatcttg ataaacgacc agcccggcaa attgaaaatt ctgatcaggt
    27181 tcaaaggtca ggatggtttc gatctggaaa tcccccgccg gggcaggcct gaggagaata
    27241 ttcgtcatgg tttcagcgtt gaccgatccg cgcccgccag cgatgcgcaa cgatcctgga
    27301 gccttgtcca aactccattg tccgggaatc tcccgcagcc aggtccaccc aggctgaaga
    27361 atgtcatcga aatcatctct aaaatagaca gggtcaacgg tcggcactat gggtgtctct
    27421 gttgtttgaa gcactggagc agcagttggg gtatttgtgg aaacgtccgg aggggtaatc
    27481 tccaacggag ttgatgttgt tgtggcatta ccactgcacg cacctgtcac aagcaacaca
    27541 gcaatgaaga caaaaattcc gataaggctt ttcgtggttt tcatcctgta aattataaag
    27601 ccgattgtat ggaaacccca acccaccttg tttttacgtg ttcataaaaa aacagcggat
    27661 accatttcgg catccgctgt tttggaagag gctcaaatca tcccttgaag aagtttgaaa
    27721 taaagaacca gatattggca ggacgttccg caaggcggcg catgaaatac gggtaccaat
    27781 gcgtcccaaa tggaacatac acacggaccg ggtatccttc tttcacgagt ctttcctgca
    27841 agtcacggcg aatgccatac agcatctgaa attcaaggcc atctttggga aggccgactt
    27901 tatcagcgta ttgttttgca aaggcaatac gtttctcgtc atgtgaagcg atcgctggga
    27961 tggcagggaa acgaccatct gctgaaagtt tcgtttgaaa ggccagggag gcatcgatca
    28021 gtattttggt caagagatcg taattcgcat ccacatctgc ttttcttgga aaagccttct
    28081 caggcggttc tttataagca cccttcacca gacgaatggt gatctgatcc tgtgtcaggc
    28141 ggcgggtatc ggcttcagcg cgataaagat aggactgaac cgccatgccc acattggtat
    28201 aacccttctc acgcatcatg taatataggt tgatggtttt atcagtatag ggcgtatctt
    28261 caatatccac gcggatgaat gtgttgttct gccgtgcccg ggccaggatt ctctcaaggt
    28321 tttcagcaca caagttttcg tcgaggccca gaccgatctg tgtcagcttg atggatacgt
    28381 taccgcgcag cgtcgcttcg gcgccaatgg catccagtgt ggcaaaaata tcatctgtgg
    28441 cttgttgtgc ttcttctggt gtattcgtat gttcccccaa atgatcgagc gtggcgttaa
    28501 tcccctttgc atttaattcc ttgacaaccc gtacggcgtc gctcagattg gtgccagcaa
    28561 caaaacggga tgcggcacgc caggcaaaac tccagttcgt aatcaggttt tgtgcccagg
    28621 ctgctttgga gaggtagata aagaaagaac ggagcatttc ggtccttttt gcgagaaata
    28681 tagagcagtt aatgtatttt agcacaagcg catggtttgg tgtgttatac ttttttccgt
    28741 gatatttgtc atttcttgga ggttctgtga gaaatcgtaa taccaacaca atccttcggg
    28801 gtgtgtcgat tctatttttg actgccgcgc tcgtactcag tatcatctcc cttattacct
    28861 acagccgcca aaggaataat tacccagcca gcatgaccat tgccggcgta cccgtcggcg
    28921 ggttgacccc agttgaggca tcccagcgcc tgctcgaagt gtacacctca cccgtggaat
    28981 tgctctacaa cgacgccatc atccacattg agcctgccgt ggtgggtttc gagcccgata
    29041 ttgaaaccat gctggccgct gccgacctta gccgaacggg cggttcattt tggggcggct
    29101 actgggattt cctctggaac cgtaccccat ctgaaattgc cattccactc agcccgaaat
    29161 tttcagaaga acgcctgcga gaatatctcc aaaatgagat tgccgcacgt tacgatgtcc
    29221 ccccctcccc ggctgtcccc gttcccggcg ggaccgattt cctgcccggc caacccgggc
    29281 agactcttga ccttgagcgc gccgtcctgc tcgtcaacga tgcacttcgc tcgccgacca
    29341 accgctctgt agccctcacc ttcacccgcg ggtcggtcgc acgtcccacc atcgacaacc
    29401 tgaccatcct gctcaaacag atcgtgcagg tctccgattt cgacggtttg atcggcctgt
    29461 atatgatgga cctgcaaacc ggacaggaaa tccacttcgc catcaacaac aaggaagaaa
    29521 tcagcgtcac acccgatatc gccttcactg cctccagtac gatgaaaatc ccagtggtgg
    29581 cttcctattt gatcaaccgt ggcagcaatc tttcgcccga tattgccaac agcatttcac
    29641 aaacgctcgg caaatcaagc aatgacgcca ctgatgttgt cctctctgcc atcgaaccca
    29701 accgcggacc cgtgatcgtg acagagaaca tgcgcagtat cggcctcgaa agcaccttcc
    29761 tggctgggtt cttcacctcc ccggcctacc tgcttccagc cattcccatc accccggcca
    29821 actcacgcac tgatgtcacc actgagccag attcatattc acagaccacc ccttctgaaa
    29881 tgggctccct cttggccgat atctaccaat gcgcccaaag caatggcggt gcactaatcg
    29941 ccgcattccc cgacaaggtc agcccgcaaa cctgtcaact gctgatcgac ttcatggccc
    30001 aggataaact cggttcgctc attcaaggcg gcgttccaga tggaactctc gtcccacaca
    30061 aacacggcta cgttcccgcc gcagacggtg tggtccacga taccagcgat gtgggcatcg
    30121 tatacacacc cggtggaaac tttgtcctct ctgtctacac ctaccatcct gtcaccaaca
    30181 tttgggatat caccaaccct ttgatcgggg atatcacgaa agccatctac aattatttca
    30241 acgtaacaag ccagtaaaaa taaccttaca gttcaataac tctccgaccc gtcttccaac
    30301 gaaaacgggt ctttttttcg cacatcgtat ataattcgac ccatgagcgc atcgttggga
    30361 ttgttccgtc tacagcaagt ggatcgtaaa attgaccgtg cgcgttcgca attggagacc
    30421 atccgcaaaa cacttgaaaa cgacaccgaa ttgcgagaat gcctcaccca tgctgaaacc
    30481 gccaaagctt ctcaccatca tgcgcatcac aacctcaaaa gcgcagaaat ggaagttgaa
    30541 tctcaaaaaa taaaaataga gcaggccgat tcaagtttat atggcggcag ggtgcaaaac
    30601 cccaaggaat tgcaagacct tcaaaaagag atcgtttcgc taaagaaaca ccttgccacc
    30661 cttgaagaac gcgaattgga agccatgctt gccgcagaga ccgccgagca aaacctgcaa
    30721 gcagccagca cacaactcga gttgatccag gctcgccttg ggagtgaaca cacaaaactg
    30781 ctcgcggatc aaaacgcact ggtagccgaa cttgaacaac tgattgaaga acgcgaagca
    30841 accttatcgc cgctggacac ttccctgctc caaatctatg aagacttgcg ccagcaaaaa
    30901 cgcggaattg cagtaacaga gatcacagac aattcctgtg ctgcctgtgg cggtacgctc
    30961 aactccgcca ttcagcaaag cgcccgttca caaaaactcg caaactgtcc cacctgcgga
    31021 cggattttat attcaaatta aacgtctccc atgtctcgat ttgcgattga cccctactcc
    31081 ttcttcgtcg gctttattgc agcatccatt ttttggctgc tggtatcccg agcgcgtccg
    31141 ctatgggccg agttggttgc gggtatcaaa gaacaacgtg aaatcgcaca aacccgcaaa
    31201 tccagcaccg tggaagaaaa tcatcgccgc atcaccctgc gccgcgcaca agggatgcat
    31261 ctcgccgcac cgcttttctc gttggatgag atcatcctcg aacctttgct gatagccccg
    31321 cctcaagttg tacaacccgg tacaccagcc aaatttgaag atgtagtttc acaaaccctg
    31381 ccctacatgc ccatgtggcc agagatcgcc gctgcatatc atgcgcaaac cctgaccctg
    31441 acacaagcct tgtctggcaa cgccaacatc gtcatcatgg gccagcccgg cgtcggcaaa
    31501 actgtggcac ttgcgcacct tgcctccctc gctgcaaatc gaagcgcaaa gctggaaacg
    31561 ctccaaaacg ccgttccatt tctctatcac gttgccaatc taaaactccc gctgcccgac
    31621 cccaaaaaag acgtccttgc tccgctcatt gaagcagcat ctgaaaacgc cccgctcatg
    31681 gacctaaacc gcctgccggg ctttttcgaa ggcgccttca aaagcggtga agccctttta
    31741 ttgatcgatg ggtttgacga aatcacagcc gaataccaac agcttgtcac cgactatttc
    31801 aaagccatta tgcaggccta cccaaagaca cgcattgtca ctacgggtgc gcccgaatat
    31861 cttgatggat taattccgct tggctttgct ccattggcat taatgacctg gtctcaacgg
    31921 gaaaatcaga aattcatcga acactggggg gaactttggt cgcaaaccgt ggcgatggaa
    31981 gcctgggcac agactggtcc tgaacaggtg gacccgctct tacttaacac ctggcttggt
    32041 actgacaatt tgaatttatc gccccttgag ttgaccctca aagcctgggg cgcctacgca
    32101 ggcgatagcc tcggcccgca cgtgctggaa gccattgcca cacacatccg ccgcattgca
    32161 cctgccaaca caccacttgc cgctcttgaa accctggcga tgcaggcgat ggtcaacacc
    32221 caacccattt ttgatccacg ggtggcccgg gcatgggtga agtcttttga agttgcagaa
    32281 gagactccca ccggcgaagc gactgaacaa gccgcggctg gagaagagac cgaagaaaaa
    32341 gcagaagaca ccaaacaact caaaggcaaa aaagtcgaaa aagtctccac acccacttca
    32401 ggtctgcttg gcaaaatggc agccagcgga ttgttggtgg tttatccgaa taacaaaatg
    32461 cgcttcaccc attcagtctt tgcaggcttt ttggctggcc gcgcattaat gagcttcaaa
    32521 gccgatgaaa ccatcctcaa tcaagcggat tggtctggca agtatctggt gatgcggtat
    32581 ttcgccgcac atggtgacgc cagtaaactg gtgcagtctc tcatggaatt ttcacgcctg
    32641 cccatgcacc gtccgctgtt tgcagcggcg cgttggctgc gtgatgcccc gcgcaatgcc
    32701 ccctggcgcg gaaaattatt cggcacactg gcggccatta tgcaaagtga aggcctgccc
    32761 cttaatctgc gtggacaagc catggcggct ttcgtcgtca gcaacgattc aagcattaca
    32821 accctcttcc gtcagcttgc gggcactctc tcttttgaac ttgttcatct ggccgtgcta
    32881 ggtgcaggtg ccatgcgtga tgcgaaagcc ctggaagttt tggagcgtgc attgcaggca
    32941 ccaagcacag ctgtacgcag ggcggcctgc atggctttgg tctcggtggg taccaatgat
    33001 gcgttggaga ctgttgctca caccctgctc agcggagagg aagatatccg ccgcgccgcc
    33061 gcagaagcac tggccaatga tccgcacgaa ggttacgcca tgctgcgtga tggggtcacc
    33121 attgacgata ttctcctgcg tcgcgctgcc gcttatggcc tgagccgtgt cagtgaagaa
    33181 tgggccattg aactgctgaa aaagatccag atcgacgaca aggaatgggt tgttcgcaac
    33241 gcggccaccg aagttctgga ctcaaagacc agtgcaggct cacttgcacc gcacaaactt
    33301 aaggcaccat ccgaatctcc ctggctgatc gaatttgccg ccaaacaggg aatgggtatt
    33361 tcccccggag tccctgccac cgatattctt ttactggctc tcaagagcga tgatgcagaa
    33421 acccgccttg cggctctgcc ttatctcaaa tacaccccca acgaaggcgt gatctcacag
    33481 ctttatgccg caatgtacaa ggatgaccat gaattgcgcg aggctgccta caatacttta
    33541 tgggaattgg gagcctccgg tgtgaagctg ccagacccca gccaatatgg atttggataa
    33601 acatgctcat aacaaacgca aaaatcatca cgtgggaaac caccaatcaa gtccttgaga
    33661 accatgcaat tctgatcgaa ggggatcgta tcaaagagat cggcaccacc aaggcgttga
    33721 cggcaaaata tccaaaagcc aagacactcg atgcccgtgg gcagtatgtg atgccgggca
    33781 gcatctgcgc gcacacacat ttttacggcg cctttgcacg cggcatggcc atccccgggc
    33841 ctgcacccaa agacttccct gagattctgc agaagttgtg gtggcctctt gaccgttcgt
    33901 tggatgcgga agcagtccgc ttttcggcgc tggtttgttt ggtggatgcg atcaaacacg
    33961 gcacaacaac tctgatcgac catcacgcct cacccaacgc cattgacggc tcactcgata
    34021 tcatcgccga tgctgtggat aagagcggct tgcgcggtgt gctgtgctac gaagtaaccg
    34081 accgtgatgg cagtgagaaa gccaatgctg gcatcaacga aaatatccgt tttttgaaac
    34141 gactggccag caacccgcat ccgcgcctcg ctggaacctt tggcctgcat gccagcctga
    34201 cgctttcggg catcaccctg caagcctgcc gcgccgccgt tcccgaaggg acgggattcc
    34261 atgtccacac tgcagaacat gaatcggatg aatatgacag cctcggcaaa agccagatgc
    34321 gtgtgatcga ccgcctgcaa aaacatggga ttcttggtcc gaacaccatc acagcacatg
    34381 gcatccactt tgatgcacgt gaaatggaaa ttctcaaaga gactggcacc tggctcaccc
    34441 accagccgcg ttcgaacatg aacaatggtg tgggcgtggc tcccgttgaa tcgatgctgc
    34501 gactgggcgt caaagtttgt ttggggaacg atggtttctc caacgccatg tgggaggaat
    34561 ggaaagccgc atacctcctg cacaagatcc accaccgcga tccacgccgc atgggcggat
    34621 atgatgtgac gcaaatggcg gtttacaaca atgccgccct ggcaaatcaa ttctttcccg
    34681 ccgccccgct cggacagatc acacaaggtg cgttcgccga tctgatcttt gtggactatc
    34741 atccctacac tccgctgacc gctggcaacc tgccatggca gatcatcttt ggatttcagc
    34801 aaagcatggt caccactact attgcagcag gcaaagtgtt gatgaaagac cgtgagttac
    34861 tcacattgga tgaggcggag atcgccgccc gcgcccgcga aattgccccg cgcatctgga
    34921 agcgctatga agaagaagta gcaaaagtaa tgtagccaga acgaaatcgt atttccatac
    34981 ttggagactt aatgaacgac tcaaatcttt tattatccat cgccgtattg ggcggcacag
    35041 gcaaagaagg caagggactc gctttccgtt gggcacgcgc agggtatcac gtccacattg
    35101 gatcgcggtc tgctgaaaaa gcgattgccg cagcgaatga gatcagggaa atgctcgggg
    35161 atgggatctc catctcaggg atgagcaacc cggaggcggc gcgcgattcg aatatcatcg
    35221 tgttaaccgt cccatattcc gcacatcgcg acaccttgga atccgtcaaa gaagaactca
    35281 aaggcaaatt gttggtggat gtcacagtac cgctggtacc gcccaaagtt gccaccgttc
    35341 aaatgcccgc tgctggctct gctgcacagg aagcaaaaca gatcgtcggc gaagaagtgc
    35401 aggtctgtgc cgcgtttcaa aatatctcgc acgaacacct actggataac tccgaggttg
    35461 agtgtgatgt gttggtcaca ggcacaagca aggaagcacg tgctgaaacc atcaaactgg
    35521 ttgaggcggc tggcttaatc ggctgggacg cggggccgct tgaaaactct gttgttgtcg
    35581 aaggacttac cagcgtgttg atcggcatca acaaaaaata cggttccacc catgctggca
    35641 tcaagatcac aggcgcgaag cgcaacacgt aattcgtaac tcaaaattcg gcgtttcgaa
    35701 ttttgggccg cgcgttacag aattttatgt ctctttccat caccccatta caaaatattc
    35761 cccttatccg ccaaggcgac aacttggcgg atattcttgt cgcttcaatt cagaattcga
    35821 ctattcaact tgaaaacgac gacatcctgg tccttgcaca aaaaatcgtc agcaaatcag
    35881 aaggccgcat ggtgaacctt gccacggtca caccatcgcc acaggcgatc gagctggctc
    35941 aaaaaaccga aaaggacccg cgcgttgtac aactcatgct gcaggaaagc aacgagatcg
    36001 tacggacacg catcggcgcg atcattgtgg agcacaaact tggttttgtg tgtgccaatg
    36061 caggcattga ccattccaac gtggccgggg acggcagcga gaatgaagag tatgtcctcc
    36121 tgctcccgga agaccccgat gcatcagcca aagccctgcg cgaccagatc aagcaaaaaa
    36181 caggcctcaa cgtcggtgtg atgatcatcg actcacatgg ccgcgcatgg agaaacggca
    36241 cggtcggaat ctgcattggc ttaagcggaa tccctgccat gatcgatgaa cgcgggtgga
    36301 aagacctgtt cggctacacc ttgaaaatca ccgtcgttgg cgtggccgat gaactcgccg
    36361 ctgccgcatc tctgatgatg gggcaggcct cagagggaat ccccgccgtg catgtgcgcg
    36421 gttttccata ccccctcggc gaaggctcac tcaaggagtt gatccgcccc aaagaacagg
    36481 atatgttccg ctaaaaaact aacacaaatc ttatccgcag ttgatacact cggtttgaga
    36541 atcaacatca tgaaaaattt tccaccagaa tatctcgacc tgctcaaaga cgaaaccagg
    36601 gcttaccttt ttcttgcaac caccatgtcc gacggctcgc cgcaagtgac accagtctgg
    36661 ttcaacatgg atgacgaata catcctcatc aacaccaacg aaggccgcac caaagaccgc
    36721 aacatgaaag cccgtcccaa agtggcattt gtcattcagg accccgaaac tccctaccgc
    36781 tatattcagg tccgcggcga agttgccgag ttcacaacac aaggcgcaga tgaacacatc
    36841 aatcagctat cgctcaaata cgatgacagg ccctggaaat atcgtgaagg acaaaagcgg
    36901 atcatcttca aaatcagacc aactcacttt gaccgccact gatctccaca cctttctgcg
    36961 gactcgacgc tcgatccgcc gcttcaaaac ggaccctgtg cctgatacgg tgatcgaaag
    37021 cattctctcc accgcgacat tttccccgtc tgcgcacaac cgccagccct ggcgttttgc
    37081 cgtcgtaaca gcatcagccg ttaaagaaaa actcgccgac gccatggccg ttgaatttca
    37141 acgcgatctc gaggcggatg cgatctcccc agaaaatgtt caaagaaaag tcagtcgctc
    37201 gcgtgaaagg atcgttgccg cccctgtggt catcatcctc agcgtagaca tgaccgatat
    37261 ggatacctac cccgataccc gtcgcaaaaa agctgaatac atcatggcca cccaatccac
    37321 cgcgaatgcg ggcatgcaaa tgctccttgc cgcccacgcc gaagggttgg ggagtgtctg
    37381 ggtatgcagc ccgctgtttg cacaggaaac tgttcaaaat gttttggggc tgcccaggag
    37441 ctgggagccg caggctatgt atttcctggg ttacccatct gaaacccccg aagtacgtga
    37501 aagaaaatcc atcaaggaaa ttgtaaaacg tgttgacgtt caaaagtcaa caccgtgaag
    37561 ctcaatccat gaaaatcact gccctcgcgg gcggagtcgg tggcgcgaaa ctggcccatg
    37621 gactcgccca aatcctccct tctcaagacc tgaccatcat cgtcaatact ggtgacgatt
    37681 tcgaacattt ggggctatcc atctgccccg atctggacac agtctgctat accctggctg
    37741 ggttggcaaa ccccgaaact ggctggggac gtgtcaacga aacctggaac accatc
//
LOCUS       DAOQJC010000024        37773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_468_length_37773_cov_10.454027, whole genome shotgun sequence.
ACCESSION   DAOQJC010000024 DAOQJC010000000
VERSION     DAOQJC010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 37773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37773
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_468_length_37773_cov_10.454027"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..94)
                     /locus_tag="PLE39_05330"
     tRNA            complement(22..94)
                     /locus_tag="PLE39_05330"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59..61),aa:Ile,seq:cat)
     gene            complement(174..245)
                     /locus_tag="PLE39_05335"
     tRNA            complement(174..245)
                     /locus_tag="PLE39_05335"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Gly,seq:tcc)
     gene            411..2990
                     /gene="gyrA"
                     /locus_tag="PLE39_05340"
     CDS             411..2990
                     /gene="gyrA"
                     /locus_tag="PLE39_05340"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNE67781.1"
                     /translation="MELGIVRKIDIDTEMQQSYLDYAMSVIVARALPDARDGLKPVQR
                     RILYAMYDMGIRADTAYKKSARIVGEVLGKYHPHGDSAVYEAMARMAQDFSMRTLLVD
                     GQGNFGSVDGDPPAAMRYTEARLTSAALDILFDLNKETVDFVPNFDDTLEEPHVLPAA
                     IPNLLVNGATGIAVGMATSIPPHNLGEIVDAIVYMIERWEKLDDIDVEQLMEFVQGPD
                     FPTGGIIIQEKGEEGIESAYGSGRGRITVQAKAHIEEMERSKSRIIVTELPYTVNKSS
                     LIERIAELARDGHLEGLSDLRDESDRQGMRIVLEMTKNADPEAVLRELYKRTPMQTTF
                     SINLLALVDGEPRTLTLKQALRVYVEHRLEVIKRRAEFDLDKAKARAHILEGYLVALK
                     NLDELIGIIRNAPDVETARQKLMKRYKLTELQANAILDMQLRRLAALERKKIETEYKE
                     VTGVIKDLTTLLKSPKLMRGVVAEELLKVKAAYGDRRRTQIVNLKKGKASKMLTATDL
                     MPDQIVWVGVTADGVIARTHDDKQPKHSGNDAPRWLVKASTSDTLYLAAENGKAAAVA
                     MHVIPEAEKLSDGVPYQKVAPLGEDDVLSAMFALPARKSEFPPETCVISITRFGMVKK
                     SLVTELPGPSSQSFVLARVNEGDGLGWVGLTDGKKKDVLLVTASGMAIRFKEDDVRPM
                     GLVAAGVNGIKLDDNDEVVGAEILPAEGEIFLLASDGKAKRVDQKDFPSQGRYGKGVR
                     AWDLPKKITLVGAVSGKPNHMATIVLAKGAPKSARLDAAAVRKRASGRGDVIVEMKKD
                     EIITGLSVAWMVDRYVKLAKTVEEGEKKEEKAKKAAVKKTPAAKAKAKPAKASAKAKK
                     KKK"
     gene            complement(3059..3730)
                     /locus_tag="PLE39_05345"
     CDS             complement(3059..3730)
                     /locus_tag="PLE39_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67782.1"
                     /translation="MFINRLELPALDLIAQRDYYANVLELPVHLEGDSLLVKAGKTDL
                     IFTQAPPQWEGAYHFCFNIPENQFPYAKAWLSARIPLVKDDKGNDEFNSQSWNSTSLY
                     FKDAAGNILEFIARHRQQNSVNSPFNSGQILQVSEIGLASQDVISFAKELCAKLGVSV
                     YEQDFNDTFTPIGDEDGLFILPVEGRIWYPNTGIPAKMLPIKVDVEANGKLFHLEGVP
                     YRIKE"
     gene            complement(3739..4308)
                     /locus_tag="PLE39_05350"
     CDS             complement(3739..4308)
                     /locus_tag="PLE39_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNE67783.1"
                     /translation="MTREEALELVREFVKNEGLVRHMLSVEAAMRFYAEKFGEDVEAW
                     GLIGLLHDFDWEIHPTLEQHPQDGAPMLRERGVPEDIIQDILSHADHLKLPRDTMRRK
                     AICACDEVTGLITAVALVRPSRSLYDLEASSVKKKWKDKAFAAGTDRSEMEHAAKEFG
                     IDIWEHVGNVITAMRKIAPELGLVGNLQQ"
     gene            complement(4360..5010)
                     /locus_tag="PLE39_05355"
     CDS             complement(4360..5010)
                     /locus_tag="PLE39_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNE67784.1"
                     /translation="MLKKIKILTWRIILTLSLIAILTLFLPRLITQLYAAPRIFTVDK
                     SPVEQVAIVFGAGLLRDGSAGPVLSDRVNTAVQLYHQGKADKLLMSGDNRFVEHNEPE
                     AMRQYALARGVPDEDIILDYAGQSTYDTCYRAREIFQVQSAILVTQSFHLPRALFLCN
                     WFGVESTGVEANNRYFRKISRLYWNFRELFATPQAAWEVLVAKPVPILGKPEPIFE"
     gene            complement(5018..6025)
                     /locus_tag="PLE39_05360"
     CDS             complement(5018..6025)
                     /locus_tag="PLE39_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNE67785.1"
                     /translation="MKKLLLILTLTILACASPINLALEGATPTPTAETNSAPTATPQA
                     TPEPGTEKNPLILALGVSAHPSTQMVDAGKVIAAYLEGRTGYRIVIVVPPTENALVES
                     LQKGNTHIASLAPFSYLLARESDTVTAIMSSVHSGQVFYGAQFIANREKDFTSFYDAE
                     RNANTAEAATALKQFEGKKPCWSDNVSPSGYVVPLGVLNQAQVKIRDGAFIESQPSVV
                     RAVYADDICDFGATFIDARELPALEYDYADVLERVVIIWRVPPVIPYEIIAMSNSLPL
                     EMRRVIQRAFVDLMLTPEGKSAVQVVYGIDEMQIAEDSNYTDFLLYVKASGLDISGLL
                     E"
     gene            6293..6973
                     /locus_tag="PLE39_05365"
     CDS             6293..6973
                     /locus_tag="PLE39_05365"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HNE67786.1"
                     /translation="MKNTFNTIMSILILVVLAAGVYFIVQTVRESAQAAADAATAPFQ
                     QVNQANQALQTQVSQMMNPTPTIIPDPVTYINEIRALARLETIQYTVEKVITAEVGQG
                     TFGFLYGDKLLFVAHGIVIAGIDMEKLQPTDMRFDGQVLYVKLPPTEVFIATLDNDKS
                     YVYDRETGALTKGNVDLETIARQSAEDEIRKAALEDGILVQGQVNAEAYLLKFFTALG
                     YKSVIFEH"
     gene            complement(7008..7709)
                     /locus_tag="PLE39_05370"
     CDS             complement(7008..7709)
                     /locus_tag="PLE39_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67787.1"
                     /translation="MKRRVLLTGLVSALLAACTAATPQPTATPTPITVPSVQPTAPIP
                     PTTTPVTASLTVKDSPINCRLGPGTVYELINELDAGENALVVGRSEDFAWWYIRDPGN
                     PDGFCWVSANVTETQGIVEELPIIQPPVTTVTDVNLRVEPNRIVINCNQFPQTVFLEA
                     EITTNGPAFVIWRWEASTGAASNDSTLVFKESGPQIINDYYQFAEPNEYWIRLHILSP
                     NELTEQVNIPVNCTP"
     gene            complement(7894..9864)
                     /locus_tag="PLE39_05375"
     CDS             complement(7894..9864)
                     /locus_tag="PLE39_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNE67788.1"
                     /translation="MSILGLAGYGFLGLLLLIFLSGIRFIPNNRIGIIEKRFTFKGSV
                     KSGLIALNGEAGYQPRILRGGLHYLMPIQYVVHTMPLVTISQGKIGYVFARDGRQLDP
                     AQVLASNAEVTDFQDVEGFLKKGGQRGPQRRILREGTYAFNLVQFIVITEERIYTLPL
                     SKEEADTIRGMAQVIAERGGFRPVVIKDTDDSIGVVTVHDGPSLAQGEIIAPVVGDDP
                     DQSVNYHNKFQDADNFLRAGGQRGRQLQVLVEGTYYVNRLFGTVEMIPKTVIEVGNVG
                     VVVSYTGETGDDLSGKEYRHGELVNKGYRGVWSEPLLPGKYPFNTYAGKVISVPTTNI
                     ILKWIRTETGAHRYDENLTEVSLITKDAFEPSLPLSVVVHIDYQKAPLVIQRFGDIKK
                     LVEQTLDPMVSAYFKNVGQMRTLIQLLQDRSAIQQQASQEMREKFAHYNLALEEVLIG
                     TPSSPENDTQIETILMQLRSRQVAEEQVETYNQQEKAAVKERELREAQSRAQMQTKLT
                     EAELNINIQTDQGKAEYQRSIQQAQQIRALAEAEAEKVARIGIAQALATEEQVRAYGG
                     PQFQVTQQVLNRFAEAIQQSGVDVVPRVVVSGSGNGEGGNSSTSSSIMEGLLTMLLSE
                     RFGAMAKESQSPRSPEADALREQIRKDMEKKK"
     gene            10084..11883
                     /gene="lepA"
                     /locus_tag="PLE39_05380"
     CDS             10084..11883
                     /gene="lepA"
                     /locus_tag="PLE39_05380"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNE67789.1"
                     /translation="MTDHIRNFCIIAHVDHGKSTLADRLLQLTGAISERDMTEQALDS
                     MDLEREKGVTIKASAVRMFYNAKDGQRYELNLIDTPGHVDFGYEVSRALKACEGAILV
                     VDATQGIEAQTLANLYQALDADLTIVPVINKIDLPSARPDEVAEDVGSLLGIEPDSVL
                     RVSAKEGINVDQILEAIVQRVPSPKDADDDPVRALIFDAHYDSYKGVIAYIRVFEGKI
                     KATDVLRMFSTQTDLRPVEIGIFAPGMKPVENIGSGEVGYIATGFKTVHECRVGDTMT
                     LASAPASEPLPGYFTPKPMVFAGIYPVEADDYTTLRESLEKLQLNDASLSFLPETSQA
                     LGFGFRAGFLGLFHMEIIQERIEREYDLDVLFTAPSVEYEVLTIGDEVVKVDSPAELP
                     DPSKIVEVREPWMNIEIITPTDYYGPIMDLVTKRRGIFKKQEYPAPHRVQLDFEIPLS
                     EIIVDFFDHLKSRTKGYASLDYQFLEYRADKLQKLEILVNGDTVDALAAIVHEKDAYH
                     KGQRLITKLKDLIPRQLYDVAVQAASGGRIISRANVKATRKDVLAKCYGGDISRKKKL
                     LEKQKRGKKRLKMVGNVEIPQEAFMAVLKLDDE"
     gene            11919..12923
                     /locus_tag="PLE39_05385"
     CDS             11919..12923
                     /locus_tag="PLE39_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNE67790.1"
                     /translation="MSQFLKDSKRWLPGALISVALIAAILYFVDINAMVNAIRSANYY
                     ILALAMSLSFVWMLGRAKVWQTLLRDKPAFRDVLFATGEGYLLNNFLPFRLGEIGRVF
                     LLSRKSGLQFGEVLPTVFIERSVDLAFSAAVFLAALPFVAGTAGSERIGIIVGIAVVI
                     GLLVMYFVARNNQWALGVFHKLSARWPSLQKFGGEFLESFFTGMGVLTDGWLFLRFLF
                     WMTLDWAIALLNYYLMLRAFFPQAEWIWGFFILGAAAFGGAIPSLPGAVGTFDGAIVG
                     ALTLLSGDQSTALAAALTARFYNYLNSGVIGGIGLMREGQTLTGVYQQIKDFRKKKEE
                     "
     gene            12920..13945
                     /locus_tag="PLE39_05390"
     CDS             12920..13945
                     /locus_tag="PLE39_05390"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNE67791.1"
                     /translation="MKKNYLVTGGAGFIGSNYVHRLLQRGETVTIFDNLSRAGAPRNL
                     EWLKKEFGANAFEVVVGDVRDADRIAEAARSADVIVHLAGQVAVTTSVTNPRNDFEVN
                     ALGTFNVLEAARSSGRNPIFLYASTNKVYGGMDDVEVVEDATRWHYKDLTYGAPETQP
                     LDFHSPYGCSKGTGDQYVRDYFRIYDLPTVVLRQSCIYGPRQFGIEDQGWLAWMIIAA
                     VTGRQITIYGDGKQVRDVLHVEDLMDAYDAAVANIHVAKGQVYNMGGGVNNVLAIWAE
                     FGPMIENLLGKKVEVARGDWRPGDQRVFYADYRKAERELGWKPKISLEEGIERLFQWV
                     KENKDLF"
     gene            14132..14944
                     /locus_tag="PLE39_05395"
     CDS             14132..14944
                     /locus_tag="PLE39_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNE67792.1"
                     /translation="MQEYFSELAAQFVASIPNIVTALLIFILSLYFARLLSNFLKRVL
                     VRRKTDSGVTQLLTQTLQWTIIVFGAITALQRFFNVTAFLTGLGIIGFTVGFALQNVM
                     QNFVSGIILLMQQPFKVGEEIRVLDFDGVVLQINLRTTELHALDGRIVILPNADVISH
                     AIVNYTRANRRRVDLPLTIASTANPEKVRSLLLEVFKDTPGYVIAPAPEINFTSFNDT
                     SIGLTASFWVDTAVLKPEHAADAALVLVKNAFEKERVASPLPAQTVFLKSEQ"
     gene            complement(15257..16228)
                     /locus_tag="PLE39_05400"
     CDS             complement(15257..16228)
                     /locus_tag="PLE39_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67793.1"
                     /translation="MNSALKILILATLFTLGACASPTEPPVPTETPIPPSPTADLGIG
                     PFLTVNKAGDLYLKSPYGAAVNQAGEIYISDAGNSRVLKFDSSGTLLAAWDKKGSGDG
                     EFNSMGFGGLAVDPSGNVFVVDNGNHRIQKFDGDGNFLTAWGTEGTGDGQFVRAIGIA
                     VDKDGNVYITDDGNPFVQKFDNDGNFLMKWGGAGSTTGKFSHATGIAVDGNGNIFVAD
                     YENKNVQKFSADGSFITTWDIRNADGKKGTPEGIVVDVQGQVYISDYDLGQIQAFTND
                     GELLWSWTDQDRNDGYFSRPTGLALDGKGMIVIVNQSGGGWEIFKLP"
     gene            16294..17460
                     /locus_tag="PLE39_05405"
     CDS             16294..17460
                     /locus_tag="PLE39_05405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE67794.1"
                     /translation="MKILTVLTYYRPHTSGLTIYAERLARAFVKRGHEVTVMTTHYDP
                     SLPLEEMMDGVKVIRIPVIARVSKGVIAPTFGLVATKLVWQHDVVQLHLPQFDAPGVA
                     MRARLFGKPVVLTYHCDVQLPQGWFNRFVNSVLDFQNDMAGRLANHIVTYTQDYADHS
                     PYLSRYASKLTPILPPVEMPVPAPEAVTAFAEKHRTKELKPVIGMVTRFASEKGVEIL
                     LDALPIILKKYPNAQVLYAGQHQNVMGEQAYFDRLIPKIHEYETSGHWTFLGNLTPVQ
                     LAALYPNLDVLTVPSLNSTEAFGLVQIEAMMNGVPSVPSALPGVRRPVQMHGMGVVSE
                     IGNAQSLAASILEVLDTPAKYRGDVDGIKTTYDPDSVAQEYEKMFERLKGRTTG"
     gene            complement(17450..17845)
                     /locus_tag="PLE39_05410"
     CDS             complement(17450..17845)
                     /locus_tag="PLE39_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNE67795.1"
                     /translation="MIYVDTSAFLAICNPEDPSYQIALDTWKNLIEKGERLASNNYVL
                     VESIALIQNRVGLEALSILHNDIIPFLEIEWLDESLHNGVMDQVITGNRRQISMVDYS
                     SFATMKRHNIRTAFTFDDHFRQHGFEIIP"
     gene            complement(17842..18099)
                     /locus_tag="PLE39_05415"
     CDS             complement(17842..18099)
                     /locus_tag="PLE39_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNE67796.1"
                     /translation="MVQLTEEQVKTLKKMARVRKASVASLVRESVTLYVDASKVNPQM
                     DEKRRRALEGLEKIKKAHFKDIEGKTDVAKNHDKYLNETYS"
     gene            18158..18919
                     /locus_tag="PLE39_05420"
     CDS             18158..18919
                     /locus_tag="PLE39_05420"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE67797.1"
                     /translation="MSQKDFLFLQVSSLPYFRAFLRAVESSYYQDLPLPAPVYDVGCG
                     DGHFASLTFDQKLDVGLDPWRFSMREAKKYNAYKSLVEADGSRAPFPSNHFASGLSNS
                     VLEHIPHIDAVLAETARVLKPGAPFYFCVPNTRYFSALSLTKVFGKPYENWFRKISRV
                     HHADEPDVWEKRLEAAGFKLERWWHYFSPASMRVLEWGHYFGLPSLVAKALTGQWILS
                     PTKWNLSLTENYLRKYASPEPVEDGTFTFYIARKK"
     assembly_gap    18960..19059
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19060..19805
                     /locus_tag="PLE39_05425"
     CDS             <19060..19805
                     /locus_tag="PLE39_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE67798.1"
                     /translation="ITNYQLPITPMSFDEKYFSTHTYANITFAKYSMYWWSNRFYAML
                     ARRYGRRGARLLEVGSGMGHLVGQLSAWFEAYGMDLNHWAVNKSKADAEAASLQTASA
                     QDLPYQNGAFNVIIIKHIVEHLPDPAKAIREIARVTEKGGILILATPNLGSLLKPWKG
                     KTWIGYQDPTHISLKQPEEWLEIIRGAGFETLRIFSDGFWDVPYIRYVPKQIQKLLFG
                     SLGGLQAITGFVFLPMRWGESIIVIARKR"
     gene            19827..22019
                     /locus_tag="PLE39_05430"
     CDS             19827..22019
                     /locus_tag="PLE39_05430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE67799.1"
                     /translation="MNEPSVLDFVKAIFKNWNSFQSFLQALFKRGDASQLMQSADEAT
                     IQLDPQPDPEPVEAPAPKKLPWRTLLVLFLALAAQQTFEPPRTSTANLGVALYIAAFG
                     LAVWAYLRGEWTMTPVSQIVPGVEDASIRRGSFVLSVIFSAATFWMMGGNKFTALNLT
                     LWVLAIVFFLRAFWLNEPRSAASSNFDLKSIFTSSEWNIRITRSALAVFVMAVIIIFF
                     RTYRLYDVPPEMTSDHAEKLMDVYDITQGQFSIYFPRNTGREPLYIYLCALFAELFTG
                     TSFLTLKVVAVIGGLLTLPYIYLLGKEFGSERIGLLAVLFAGIGYWPSVIERFGLRIS
                     FYPLFAAPTLYYIIRGLRRQNRNDFILAGIALGVGLNGYTPFRIMPFVVVALFGVYLL
                     HARDWQARKQSIIWLALLAFTSFVFFIPLARYMIEFPDMFVYRAFSRLSTVERPLPAP
                     IWQLIPLNLWNAAKEFNWYNGDIWVHSIPGRPALDIVSGALFLLGVSLMLFRYIRSRS
                     WMDLMLLLAVPFFQMPSILSLAFPEENPSLNRTGAAIVPVFLLVAIGLDTLLNSINLR
                     EKANEESIMSENRRAMGHRPLLAGTIFVLLFLFSFTQNYNLVFHTYYDQYRASAWNSS
                     EMGAVMKGFMERGGSADNVWIVPFPFWVDTRLPPFWAGVPGRDIAIAPDNIPATVELS
                     GDKLFMFTLDDTVTMSELKRLYPQGTLSQFHSAVPAHDFYVYQIPASQ"
     assembly_gap    22236..22335
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22336..23907
                     /locus_tag="PLE39_05435"
     CDS             <22336..23907
                     /locus_tag="PLE39_05435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE67800.1"
                     /translation="VQKMDWEWLVLLLAVFVFAAFFRFYRLNEVLAEPFSDHAEKLLD
                     VYDISKGNTSIFFIRNTGREGLQMYWTFLVAKIFGTGISFFSLKLGTALIGLFTLPFV
                     YLLGKEFGNPVVGFFAMFLFGISYWMNVTARIGLRFPLYPLFAAGTLLFLTRGLRTAS
                     RNDFLLCGLLLGLGLHGYTPFRIMPFVVLTAFAIFILHNNSKAIRVQAVQGLTIIAVT
                     ALMVFLPLLRYWLEYPEVFGLRAFSRLDSAGTFGSLMGVFFSNLANALLMFNVDDGNI
                     WVNSLPHRPALDVVTGAFFVIGLVLLGLRYLRQRDWRDLFLLVLIPLLLMPSVLSLAY
                     PEENPALNRAGGAAVAATLIAALAVEGLVVGTVRGVESGQGPEKRRGFILHILVGVLL
                     AAASFQNYDLVLRQFDRYFRIGAWNSSEMGRSIKFFGDVYGRTDTAWIVPYEQWVDTR
                     LPALWMGDPNRDFALWPSQFADTLAMPAPKMFIFNLRDLDTKNALKQLYPNGTLSRYT
                     SATTGKDYMIFFVEE"
     gene            23964..28865
                     /locus_tag="PLE39_05440"
     CDS             23964..28865
                     /locus_tag="PLE39_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNE67801.1"
                     /translation="MTEKRAWFYDLLFVCILLIAGFLRLTGVEWGEGYHQHPDELFLV
                     GVLDNLRAHTCADAVTPVDLCKPEDQRWMTLGEYFDTATSTLSPYNRGHAFFVYGNLP
                     MTVTRIAMEMTGNDDIGKSKFFARQFSALADLFTIFILYLIVSNLYGRRIGLFAAVFS
                     TFAVMQIQQSHFFTTDLFVNLFMFLAIMFAMGIVEKRDLGIGNNESETLQLTSSDAGF
                     SNYLLRLVRSPLFLLSIGFGFALGMAMASKINAAALAILLPGAFAVRFLVQDKDKKLA
                     ADYWSLITVFLIAGGLATIISFRIFQPYAFNGLGLNDQWLSNIAEQRVQANGDADLPW
                     NLQWARRTHLYSFTNLTVWGLGLPLGILAWVGFLLMGWRILKGEWRHLLLWGWTAFYF
                     LWQSLQFNATMRYQLPIYPLLCMMAAWALFELPKLRGETGRFKLFEVLVATMGVVVLV
                     TTTIWAFAFQSIYTRDEPRMAASRWLFQNAPGPVNVKIKMADNTIYNQPLPVPMDMAI
                     QAGAPFTMTFSANRDGDISEIVFGHAIDLESPSTIVLTLASASAPDLVLARASEKVDY
                     SQTKDPRGPAVEFKLDETVPLVKGQAYLLKIETLGHALTLSGSAIANETGYDWGLPFR
                     IDAYDAFGGIYRGDLTLEVYWDDNPDKVNRYVSMLSMADYIVIPTNHQYAQITRLPER
                     YPLTTYYYRKLLGCPENADIIECYRNAKPGQYEGGLGFELVKVFETYPQFGPIIINDQ
                     AAEEAFTFYDHPKVLIFKKTSDFNADKLSAMLNTVDLTNVVHLTPKGADNYKSLMLSK
                     EQYAAQRAGGTWSQLFNYEWLQNKYPVIGLLLWYAFIFLLGLAAYPIVRLALPGFRQY
                     SYPLGRMAGLSLLAWISWMGGSLGVPYTRGTIGAAFVLVTVAGLGLWWIRKAQFKDEW
                     DSNRKFFTLVEIVFAVFFLIDLLIRFGNSDLWHPSKGGERPMDFSYFNAILKGTSFPP
                     YDPWFAGGYINYYYYGFVLAGTPVKLLGIVPSIAYNFILPTWFALVAVGAFAIGWNLL
                     EKEEGTSPNFSAQHSSLLSGLSAAVLSVLLGNLGTIQMIFQALQRMAAPGGIITDATF
                     VQRWIWAFQGFMMSFGPGASLPIGRGEWYWNPSRVIPPGPGNEITEFPLFTFLYSDLH
                     AHMLVMPLALFVIAWALSFIKSRANLRREEWAASFLLGAMVLGAVYPTNLSDFYTYLL
                     LALVVLAFVFLFRAEVSGLRLLEDLPDAVKRIALMLVSIGALTVLSFVMYQPYRSAYS
                     QSYGALDPWTASHTPVSSYLTHWGLFLFIIFAWMAWETREWMARTPLSSLASLRPFAI
                     LIELALAGIFVVLVYFAYEGIWVGWLALPLAAWAGVLLLRPDQPDEKRLVLFLIGTAL
                     MITLVVELRTVRGDIGRMNTVFKFYMQAWLMLSVSAAAALGWMFSDAPRWSAGWRNTV
                     QVILLTLVFGATLFTFTATSDKVTDRASTDTPRSLDGLNYMNYSTYFDHGQNLTLNED
                     YRAIRWMQDNVEGSPVIVEAAPAGRQYEWHSRFTIYTGLPGVVGWQWHQQQQRVLFSQ
                     QIIDRGIEVDAFYNSLDQDSVRKFLAKYEARYIIVGQLEQAKYMPIDPGIPNGLMKFD
                     LWNGTLWNEVYRDGDTVIYEVIQ"
     gene            28862..29218
                     /locus_tag="PLE39_05445"
     CDS             28862..29218
                     /locus_tag="PLE39_05445"
                     /inference="COORDINATES: protein motif:HMM:NF017880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HNE67802.1"
                     /translation="MILHITSRAEWSAAQARGEYIAPSLQTEGFIHCSTDKQVAHVAN
                     AFYRGRNDLVLLMIDEAKLKPELKWEPPAGPPAPGISESDSFPHIFGPINLTAVASVL
                     DFEPDPASGTFSYPKA"
     gene            29215..29832
                     /locus_tag="PLE39_05450"
     CDS             29215..29832
                     /locus_tag="PLE39_05450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNE67803.1"
                     /translation="MTEPAAALRIRPAHAADSALAAKVIYMSMGGLADYLFDNNGQAI
                     QTILAELFTRDAGRFGFRIAMVAEDETQPVGMLFACPGARLAGFNMASFQHFFPVMGF
                     KHALKFMWRGVTLPGGPEAEKDEYYVSNLGILPEAQGRGIGTSLLAYAEETCRAHQLS
                     KCSLIVGMHNENAFRLYQRLGYQVVETAHAPNEHLAYYRMVKHLN"
     gene            29864..32380
                     /locus_tag="PLE39_05455"
     CDS             29864..32380
                     /locus_tag="PLE39_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNE67804.1"
                     /translation="MTAFIAWYFILTLLGWLTFPLAHFLFPALKDRGYTLSRAAGLLI
                     WGYSFWLMASLGIAQNDLGGILLALIVLASLSAWVLANQRSEIIDFFRTHRSLVITTE
                     VLFLVAFAFLAFVRSANPELTGTERPMELAFINAILRSPVFPPQDPWLSGYAISYYYF
                     GYVMTAMLAKVSGIYGSMAHNLMTALVFALGAIGSYGILYNLLSHGHEPKKTEDGDMD
                     TAGSHRSSFSIYGFLSLLAPLFLLLISNLGALLEVMHQWGLFWKQDYISGEYTSAFWK
                     WMDMKELSQSPILPFHAPPLDRYLWWWRSSRVLQDYDMLNRPLEVIDEFPFFSFLLGD
                     LHPHVLAIPFGLLAIAAALNLFLGGWRGEVKLPLGARLQISGPGFLFTALLLGGLAFL
                     NTWDILIAAALIVASYVLWCANEEGWRWDRLEDALLFGFPLGILAILMYLPFYVGFSS
                     QAGGILPNFMYPTRGIHLWVMWGTLLVPILAYVLYLWRGEKFPANWKLGVSLGLGFVV
                     FLLVFSWLLGVIGSILEKDFVNGFLASQGMTASQFFGATLMRRLSYIGGLITMLAVFI
                     PSLAFLFSQKQQGQPEAELSAPLEDNRKPETQFVLLILILGAILIITPDFVYLRDKFG
                     YRINTVFKFYYQAWMLWSLAAAFAASRLLQSLRGAANVAFRVALVLVVSTGLLYPVLG
                     LITKTNNFNPPYGFALDDFERVIRETPEEAAAINFLRTAPDGVVAEAIGDSYNYPFTR
                     ISTHTGLQTVLGWPGHEDQWRGSYIPQGSRRDDINRLYTTVRWEEALQIIEQYNIRYI
                     FIGTLERTSMNVSEEKFIAHLKPIFQQGGTVIYEVPVLEK"
     gene            complement(32427..34118)
                     /locus_tag="PLE39_05460"
     CDS             complement(32427..34118)
                     /locus_tag="PLE39_05460"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE67805.1"
                     /translation="MTTDFSSLNLREEITQAITELGYSEPTPIQAGMIPLMLTGVDVI
                     GQAQTGTGKTAAFALPILNNFQHQKNPQALVLAPTRELALQVADSMTEYGKHLNLRVL
                     AVYGGQPYSPQINSLKRGVDIVVGTPGRLNDLLERGVLILNEIKTVVLDEADEMLNMG
                     FIEEVEKILSMTPSERQTALFSATMPPRIRALANRFMRDPQSVVIKRSTLTAAAIEQR
                     YYLVHDNDKINALTRLFEVEPIHSALIFARTRAETGQLANELVIRGIPAEAIHGDLDQ
                     NARERVLGRFRANQLKVLVATDVAARGLDIDDISHVFNFHLPDDAEVYVHRIGRTGRA
                     GKTGIAISLISPREKRRMREVEALTKQLVTKMELPTASEIMRHRESLVVENLKIWLGR
                     ARYKRELEMVQELIEAGHDVMNIAAAAIKIARADEKQRPIAEVSDVKTDFTRKPEREF
                     GRRDSKGGKREFGAGSAAKGSKREFGDKKSRRDSHEEGMVRLKINKGKAHNIRPNDIV
                     GQIAFHANIPGYTIGKIRIEEKVSFVDVPADAVDQVLKHSGNYKIGKEKFSVAKP"
     gene            34405..34809
                     /locus_tag="PLE39_05465"
     CDS             34405..34809
                     /locus_tag="PLE39_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNE67806.1"
                     /translation="MAAKNVHLNWNPDQQFTLQDNDGYQLVMKKPKGVSASDLLPMAL
                     IGCASHDVVEILNKQKQDLRQLNVTAESIQDETPPWRFRKIHIHFQVVGKGIDPEKVH
                     KAIQLSEEKYCSVYATLKDAVEIVNDFEVVEA"
     gene            complement(34899..35699)
                     /locus_tag="PLE39_05470"
     CDS             complement(34899..35699)
                     /locus_tag="PLE39_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67807.1"
                     /translation="MKLKQRFPFLVLITLSAALMIVLPLIRFFTLQPQQDALQQLEPS
                     FQFRVFFLGLADGLWKAVLILLCFWSGVGINRLLLRIQDSAKRKLAINGVIFGSLFLC
                     VVLPILGLILTYTFFSPKESWVQLPDPPEAARTISGGIYNRIIIETEKGNYFSHTIPD
                     DGLGWLADEKPDASLLPEMDESTSPDIEAPGVFTNLIGVPAYPGSTQKIYYAILEDHT
                     VWYLPQTGNTFFTTVLLAAVSIPILIGGLLMLLGMGTISILDKLAERI"
     gene            complement(35860..36189)
                     /locus_tag="PLE39_05475"
     CDS             complement(35860..36189)
                     /locus_tag="PLE39_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67808.1"
                     /translation="MKKANKTSQTEDMLPEYNLEGKKGVRGKYVKALQAGYSVRVMHE
                     DGTVTMRHFVPKESAILLDKDVKAHFPDSKSVNAALRSLIRKRAKVVVEKQKKVRSIA
                     AKARRSK"
     gene            complement(36173..36469)
                     /locus_tag="PLE39_05480"
     CDS             complement(36173..36469)
                     /locus_tag="PLE39_05480"
                     /inference="COORDINATES: protein motif:HMM:NF016268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNE67809.1"
                     /translation="MKIIFEWDENKARTNFEKHKVSFDEARTIFVDPLLVTFADELHS
                     EKEERFISIGLSENNRILLVVHTEREEKKETIIIRIISCRKATPTERKRYEKGE"
     gene            36900..>37773
                     /locus_tag="PLE39_05485"
     CDS             36900..>37773
                     /locus_tag="PLE39_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67810.1"
                     /translation="MTVAICIYWLFSGTFDISNDLGGVAGIIAVPLLFTIAAFRSYIL
                     QNMPSATEAVEQDPRLPVLYLRSFDQEMLKFVHLKDEEKARYNTFLNYAGDVNLPPFE
                     QAIYTALNSSALLTFGSVESEDVTFEKYFRGEVMERIGPFVALGNPIDSLPTEGAFRD
                     YQIDERWKDVFFNHSERAACILMQLGSSDNLQYELTAILSRHISHKLFILTPPQENLK
                     DRYAWVRKYILREKPANWQAFSAILQKNGFRIPDEPLGIGSVLTFEKDGSPLVLVRNA
                     FQPQEYIQPIAERLK"
BASE COUNT         8295 a         9601 c         9866 g         9811 t
ORIGIN      
        1 atggggcgtg cgaacaattt gtgggcccgg caggattcga acctgcgacc aagcgattat
       61 gagtcgcctg cgctaaccgc tgcgccacgg gcccgtgtta agtaccaagt gttccagcgt
      121 catgtgccag gcaaactgac accaaagcac ctgacactgg aacactgatg aagagcggga
      181 gatgggattc gaacccacga atatcagctt ggaaggctga tgccttacca cttggcgact
      241 cccgcattgc gagcgtgatt ttactgtgag gtgtgaaccc agtcaagcca cttgacgcct
      301 tccatctttc cccgtaagat aagccccgca atagaacata aattcaccac aaatagcgca
      361 aagttacgaa aaaatttctt tgtatgccat tgtggttatg aggaactctt atggaactcg
      421 gtattgtcag aaaaatagac attgacactg aaatgcagca atcgtatctc gattacgcca
      481 tgagcgtgat cgtggcgcgc gccctgcccg atgcgcgaga cggacttaaa cctgtgcagc
      541 gccgcattct gtatgccatg tacgacatgg gcatccgcgc tgatacggct tataagaaat
      601 ccgcccgtat cgtgggtgaa gtgctcggta aatatcaccc gcatggcgat tcggctgtat
      661 atgaagccat ggcacgcatg gcgcaggatt tttccatgcg cacgctactt gtcgatggtc
      721 agggtaactt cggttccgtg gatggcgacc cgcctgctgc gatgcgttat accgaggcgc
      781 gccttacttc tgctgcactc gatatcctct ttgacctcaa caaggaaacc gttgacttcg
      841 ttccgaattt cgacgatacc ctcgaagagc cgcatgtatt gcctgctgcg atcccgaacc
      901 ttttggtgaa tggcgcaacg ggtattgcgg tgggtatggc tacatccatt ccgccgcaca
      961 acctcggcga gatcgtagat gcgatcgtgt acatgatcga gcgttgggaa aaactggatg
     1021 acatcgacgt tgaacagttg atggaatttg tgcaaggccc cgatttcccc acgggcggca
     1081 tcatcattca ggaaaaaggt gaagagggca ttgaatccgc atatggctcg ggacgcggac
     1141 gcatcactgt gcaggccaag gcacacatcg aagagatgga acgcagcaag agccgcatca
     1201 tcgtcaccga attgccgtac acggtcaata aatccagctt gatcgagcgc attgccgaac
     1261 tggcacgcga tggtcacctc gaaggcctct ctgacttgcg cgacgaatcc gaccgacaag
     1321 gcatgcgcat cgtccttgag atgacgaaaa atgccgaccc ggaagctgtt ctgcgcgaat
     1381 tgtacaaacg caccccgatg cagaccactt tcagcattaa cctgctggca ttggtggatg
     1441 gcgaaccccg tacactcact ctcaaacagg cgctgcgtgt ttatgttgag caccgactcg
     1501 aggtcataaa aagacgcgct gaatttgatc tggataaagc caaagcccgc gcccatattc
     1561 ttgagggata tctggtcgca ttaaagaatc tggatgaact tatcgggatt atccgcaacg
     1621 caccagacgt tgaaactgcc cgccagaaat taatgaaacg ctacaagctg acagagttac
     1681 aggccaatgc cattctcgat atgcaattgc gccgactggc cgctcttgag cgcaagaaga
     1741 tcgaaaccga atacaaggaa gtgaccgggg tgataaaaga cctgaccaca ctcttgaaat
     1801 cgccgaaact gatgcgcggt gtggtggctg aagaattgtt gaaagtgaaa gccgcttatg
     1861 gcgaccgccg ccgtacgcag attgtcaatt tgaagaaagg caaagcctcc aagatgctca
     1921 ctgccaccga cctgatgccc gatcagatcg tatgggttgg tgttaccgcc gatggtgtca
     1981 ttgcccgcac acatgatgat aaacagccca agcattctgg taatgatgcc ccgcgctggt
     2041 tggtgaaggc ctccacttct gatacgcttt accttgcagc agaaaacgga aaggccgctg
     2101 cggttgcaat gcatgtcatt cctgaagcgg aaaaattgag cgacggtgtg ccgtatcaaa
     2161 aagttgcgcc gttgggcgaa gatgatgtac tttcggccat gtttgctttg cctgcacgga
     2221 aatctgaatt cccgcctgaa acttgtgtca tttccatcac gcgctttggc atggttaaga
     2281 agagtttggt taccgaactg cccgggcctt cttcacagtc gtttgtcctt gcaagggtca
     2341 atgaagggga tgggttgggc tgggtcggtt tgaccgatgg aaagaaaaag gatgttttgc
     2401 tggtcacagc cagcggcatg gcgatccgct tcaaggaaga tgatgtgcgc ccgatggggc
     2461 ttgtcgctgc tggtgtgaac ggaatcaaac tggatgacaa cgatgaagtg gttggtgcag
     2521 agatcctgcc cgccgaaggg gagatcttcc tgctggcgag cgatggcaag gccaagcgtg
     2581 tagaccagaa ggatttcccg tcacaaggcc gctatggcaa gggcgtccgc gcctgggatt
     2641 tgccgaagaa gatcacgctc gttggcgcgg tcagtggtaa accgaatcac atggcgacca
     2701 ttgtccttgc gaagggtgct cccaaatctg cacgattgga tgcggctgcc gttagaaagc
     2761 gcgcctctgg ccgcggcgat gtcatcgttg aaatgaagaa ggatgagatc atcaccggcc
     2821 tgtctgtggc gtggatggtg gatcgttatg taaaactggc caagactgtg gaagaagggg
     2881 agaagaagga agaaaaagca aagaaggccg ctgtaaagaa gacgcccgct gcaaaagcga
     2941 aggcaaaacc agcgaaggct tccgcaaagg caaagaaaaa gaagaaatag gaaagagaaa
     3001 acccgcatct gagacaaaac agatgcgggt tttgtttttg aaaagggtaa gccaattttt
     3061 attctttgat tctgtaaggc acgccttcca gatgaaacaa cttcccattc gcttccacat
     3121 ccactttgat gggcaacatc tttgcaggga tgcctgtatt gggataccaa atgcgtccct
     3181 caactgggag aatgaataga ccatcttcgt cgccgatggg ggtgaaggtg tcgttgaaat
     3241 cctgctcgta cactgaaaca cccagctttg cgcataattc ttttgcaaat gagatgacat
     3301 cctgcgaagc cagcccaatt tcgctgactt gcagaatttg tccgctgttg aatggggagt
     3361 tgacgctgtt ctgctgtctg tgacgggcga tgaactcaag aatgtttccc gctgcatcct
     3421 tgaaatacag ggatgtggaa ttccatgact ggctgttgaa ttcatcattg cctttgtcat
     3481 cttttacgag cggaatcctt gctgagagcc aggcttttgc gtacggaaac tgattttcag
     3541 ggatgttgaa acagaagtgg tatgcacctt cccactgtgg cggtgcctgg gtgaagatca
     3601 gatctgtttt cccagctttc acaagcaaag aatcaccctc aagatgaaca ggcagttcca
     3661 aaacgtttgc gtagtaatcg cgttgggcga tcaggtccaa agcgggtagc tcgagtctgt
     3721 tgataaacat atgaccagct actgctgaag attcccgacc aaacccaatt caggtgcgat
     3781 cttgcgcatg gctgtgatga cattaccgac atgctcccaa atatcaatcc caaattcttt
     3841 ggcggcatgt tccatttcag agcggtcggt gcctgcggca aaggctttgt ccttccattt
     3901 ctttttgacg gaactggctt caaggtcgta aagcgaacgg gacgggcgca ccaatgccac
     3961 agcggtgatg agacctgtta cttcatcaca ggcacaaatg gctttacggc gcatcgtgtc
     4021 gcgtggaagt ttcaaatggt cggcatggct gagaatatct tgaatgatat cttcagggac
     4081 gccgcgctca cgtaacatgg gcgcaccatc ctgtgggtgc tgttcgagcg tggggtggat
     4141 ctcccagtca aagtcatgca gcaggccgat caagccccag gcctccacat cctcgccgaa
     4201 tttttcagcg tagaaacgca tggcggcttc caccgaaagc atgtggcgca caaggccttc
     4261 gttttttaca aactcacgga cgagttcaag ggcttcttca cgagtcattg gggttctcct
     4321 taataaggtt gcgcgagaca gcatcccgcg ttacgaaatt tattcaaaga tgggttctgg
     4381 tttgccgaga atgggtactg gttttgcaac caacacctcc catgccgcct gcggagtggc
     4441 aaacagttcg cggaagttcc aatacaatcg cgatattttg cgaaagtatc ggttgttcgc
     4501 ttccacacca gttgattcaa cgccgaacca gttgcacagg aacaaggcac gcggcaggtg
     4561 aaaactttgc gttacaagaa tggccgattg cacttgaaat atttccctgg cgcggtagca
     4621 ggtatcgtat gtgctctggc ctgcataatc cagaataata tcttcgtcgg gcacaccgcg
     4681 tgcaagcgcg tattggcgca tggcctcggg ttcgttgtgt tccacaaagc gattgtcgcc
     4741 gctcattaac aacttgtcgg ccttgccttg gtgatacaac tgaactgccg tattgaccct
     4801 atcacttaac accgggccag ccgagccgtc gcgcagcaag ccagcaccaa acacaatcgc
     4861 cacctgctca acaggagatt tgtccacggt gaagatgcgt ggggcggcgt aaagctgggt
     4921 aatgaggcgg ggcagaaaaa gtgtgagaat ggcgatcagg ctgagggtca gaatgatgcg
     4981 ccatgtcaga attttgattt ttttcaacat ggaggagtta ttcgagcaat cccgatatat
     5041 caagccccga ggcttttaca tagagcagaa aatcggtgta gtttgaatct tcggcgatct
     5101 gcatttcatc aatgccgtac acaacctgca ccgccgattt tccctcgggg gtgagcatca
     5161 ggtcaacgaa ggcacgctgg atcacgcggc gcatttcgag cggcagcgaa ttggacatgg
     5221 cgatgatctc atatggaatg acgggcggca cacgccagat gatgacgacg cgttcaagca
     5281 catcggcata atcgtattcg agtgcgggca attcgcgggc gtcaatgaac gttgctccaa
     5341 aatcgcagat gtcatcagcg taaacagcgc gcaccacact gggttgactt tcgatgaaag
     5401 ccccgtctct tatcttaacc tgggcctgat tcagcactcc gagcggcacc acataccccg
     5461 aaggtgaaac gttgtcgctc cagcagggtt tctttccctc gaattgtttc aaggcggtgg
     5521 cggcctcagc cgtgttggcg ttgcgttcgg catcgtaaaa agaggtgaaa tctttttcgc
     5581 ggttggcaat gaattgtgcg ccgtaaaaga cctgccccga atgtacactg gacatgatgg
     5641 ctgtaactgt gtcactttcg cgcgcgagca ggtatgagaa tggcgccagc gatgcgatgt
     5701 gggtatttcc cttttgaagc gattcaacga gggcattttc agtgggcggc accacaatga
     5761 caatacggta gcctgtgcgc ccttcaagat acgcagcgat gaccttgccc gcatccacca
     5821 tttgggtgct ggggtgtgcc gataccccca aagcaaggat cagcggattc ttttcagtgc
     5881 caggctcggg tgtggcttgt ggcgttgcag tcggcgcgga attagtttca gcagtggggg
     5941 tcggtgtggc accttccaaa gccagattga tgggagaggc acacgcgaga attgtgaggg
     6001 tcaggataag caggagtttt ttcattttca aaattgtaca tgcataattg cgcgatgttg
     6061 ctataataac tcaccttgca tggattctat aactattcgg gagtggctaa aagaatgggc
     6121 tttgactgcc acaaagtcac aaaggctcga aggcacaaag gattcttggt ggcttagtgg
     6181 cttggcgtct ttgtgattag cctatggact tggtcactct caactatccg tgtggaacaa
     6241 aatcgggcac aagtgcgtat atcattaata aggagattaa gaaaggacaa tcatgaaaaa
     6301 tacattcaat acaatcatgt ccatcctcat tttggtcgtg ctggctgctg gtgtctattt
     6361 tatcgtacaa actgtgcgtg agagtgcgca ggctgccgcc gatgctgcca ctgcaccatt
     6421 tcagcaggtg aatcaggcga accaggcttt gcagacgcag gtatcgcaga tgatgaaccc
     6481 cacccccacc atcatcccag acccggtgac ctacatcaat gagatccgtg cgttggcacg
     6541 tttggagacg atccaataca cggtggagaa agtcattacc gccgaggtgg gtcagggaac
     6601 ctttgggttt ctgtatggag ataaactgct gtttgtggcg catggcattg tgatcgcagg
     6661 cattgacatg gaaaagctcc agcccaccga tatgcgcttt gatggacagg ttttgtatgt
     6721 gaaattaccc cccaccgagg tttttattgc cacacttgac aatgataaat cctacgtgta
     6781 cgaccgtgag accggcgccc tgaccaaggg caacgtggac ctcgaaacca ttgcccgtca
     6841 atcggctgag gatgagatcc gcaaggccgc gttggaagat ggcattctgg ttcagggtca
     6901 ggtcaatgcc gaggcgtatt tgctgaagtt ttttacggca cttgggtata aatcggtgat
     6961 cttcgaacat taagatggag tgcggacgaa aagtccgcac tcttctttta tggggtgcaa
     7021 tttacaggga tgttcacctg ttcggtcaat tcatttgggc tgagaatgtg caggcggatc
     7081 cagtattcgt tcggttctgc gaactggtaa taatcgttga tgatttgtgg acccgattct
     7141 ttgaatacca atgtcgagtc attggaggcc gctcccgtgc tggcttccca tctccaaata
     7201 acaaaagcgg ggccattggt ggtgatttcg gcttcgagaa agaccgtttg tgggaactgg
     7261 ttgcagttga tcacgatgcg gttcggttcg acccgcagat tgacatcggt tactgtagtg
     7321 accggaggct gaatgatcgg taattcctcc acgattccct gggtttcagt cacattggcc
     7381 gaaacccagc aaaacccatc cgggttgcca ggatcgcgga tgtaccacca ggcaaaatct
     7441 tcacttctgc cgactacgag ggcattttct cccgcatcaa gctcgttgat taactcatat
     7501 acggtccctg gcccaagcct gcaattgatg ggggagtctt ttacggtcag gcttgccgtc
     7561 acaggagttg tagtgggcgg gatgggagca gtaggctgca cactgggaac ggtgattggc
     7621 gtaggcgtgg cagtaggctg tggcgttgcg gcggtacagg cagccagtaa agcagagaca
     7681 agtcctgtca gcaggacgcg gcgtttcatg tttcctcctt ggaacataac aatctgcttg
     7741 ctgtaacatt aattatccgt atttggtgcg cagtttcaag ttaatttttg aaaatcagta
     7801 cttgagagtg ggttattttc agtgtttgta cgtggaaaat gaaaactccc ccgcaaaagg
     7861 tacattgcgg gggagtttgt atataacctg ggcctacttc ttcttttcca tatccttgcg
     7921 gatttgttca cgcagggcgt ctgcttctgg agatcgcggg gattgagatt cctttgccat
     7981 ggctccaaaa cgttcggaga gtagcatggt cagcagtcct tccatgatgc tgctggaggt
     8041 ggaactgttg ccgccctcgc cgtttccgct gccgctcaca accacacggg gaaccacgtc
     8101 cacaccagat tgttgaatgg cttccgcaaa acggttgagt acctgctggg ttacctggaa
     8161 ctgcggcccg ccataagcac ggacctgttc ttcggtggca agcgcctggg cgataccgat
     8221 gcgagcgacc ttttcggctt ctgcttcggc caatgcgcgg atctgttgcg cctgttgaat
     8281 ggagcgttga tattcagcct taccctggtc ggtttggatg ttgatgttca actccgcttc
     8341 cgttagtttg gtctgcattt gcgcacggga ttgggcttcg cgcagttcac gttccttcac
     8401 ggcagccttt tcctgctgat tgtaggtttc aacctgttct tctgccacct ggcgcgaacg
     8461 taactgcatc aggatggttt cgatctgggt gtcgttttct ggtgaagacg gtgtaccgat
     8521 cagcacttct tcaagtgcaa gattgtagtg cgcaaatttc tcacgcattt cctggctggc
     8581 ctgctgttga atggcgctgc ggtcttgcaa taattggatt aaggtacgca tctggccgac
     8641 attcttgaaa taggcagaga ccatcgggtc gagggtttgt tccaccagtt tcttgatgtc
     8701 accgaagcgc tggataacga gcggggcttt ctggtaatca atgtggacca ccactgaaag
     8761 cggcagggac ggctcgaagg catctttggt gatgagcgaa acttcggtca ggttttcatc
     8821 gtagcggtgt gcgcccgtct ccgtgcggat ccacttgaga atgatgttgg ttgtcgggac
     8881 agagataacc tttcccgcat acgtgttgaa tggatacttg cccggcagaa gaggttcact
     8941 ccacacaccg cggtagccct tgttaaccaa ttcaccgtgg cgatattcct tgccagaaag
     9001 atcatcaccg gtttcacctg tgtaggaaac gaccacgccc acattaccga cctcaattac
     9061 ggtcttgggg atcatttcca cggtgccaaa caggcgattg acgtaataag tgccttccac
     9121 caatacctgc aactgacgac cgcgctgacc gccggcacgc aggaaattat ccgcatcctg
     9181 gaatttgttg tgataattca cggattgatc gggatcgtca ccaaccactg gggcgataat
     9241 ctcaccctgt gcgagtgatg ggccgtcatg tacggtcaca acaccgatcg aatcgtctgt
     9301 gtccttgatg accacagggc ggaaaccgcc acgctctgca ataacctgtg ccatgccgcg
     9361 gatggtatcc gcttcttcct tgctgagagg cagggtgtag atacgctcct cggtgatgac
     9421 gatgaactgc acaaggttga acgcgtaggt accttcacgc aaaatgcggc gttgtggacc
     9481 acgctgtcca cccttcttca agaaaccttc cacatcctga aaatcagtta cctccgcatt
     9541 ggatgccagc acctgtgcag gatccaattg cctgccgtca cgggcaaata catagccgat
     9601 cttgccttgg gaaatcgtca ccaacggcat ggtatggaca acatactgaa tgggcatcaa
     9661 gtagtgcaga ccaccacgca ggatgcgcgg ttgatacccc gcctccccgt tcaaagcgat
     9721 caggcccgat ttcaccgacc ccttgaatgt gaagcgtttt tcgatgatac cgatgcggtt
     9781 attgggaatg aaacggatcc cggaaagaaa gatcagaagc aaaagaccca aaaaaccata
     9841 acctgccaaa cccaaaattg acattttttc tcctttgtgc gtcgaattac gcacaataca
     9901 taaatacgaa ctggccccat cagggttgct tgaattctaa accctttcat ttttgcgggt
     9961 atcgcccttt gatactaccc agcgggcaaa atgggaaaaa taaatcggtt atacttcgtt
    10021 cgcttacagg gcagaaacct tccctttgta gtaaatttaa aaaaacgaaa tttcgaaagc
    10081 agcatgaccg accatatccg taacttctgt atcatcgccc atgttgacca tggcaaatcc
    10141 accctcgcag accgcctgct tcaattaaca ggtgccatct ccgagcgtga tatgaccgaa
    10201 caagccctcg actcgatgga tctcgaacgc gaaaaaggcg ttaccatcaa ggcctcggcg
    10261 gttcgcatgt tttacaacgc caaagacggc caacgctatg aacttaacct tatcgacacc
    10321 cccggccacg tggattttgg ctatgaagtc agccgcgcac tcaaagcctg cgaaggtgcc
    10381 attctggtgg tggatgcgac tcaggggatc gaagcccaaa cgctggcgaa tctatatcag
    10441 gcgctcgatg ccgacctgac aattgttcct gttatcaaca aaattgacct cccctcagcc
    10501 cgtcccgatg aagtggctga agatgtaggc tccctgcttg ggattgagcc agattcggtt
    10561 ctgcgcgttt ccgccaaaga aggcatcaat gtagaccaga tcctcgaagc cattgtccaa
    10621 cgtgtgcctt ctcccaaaga tgcagatgac gatcctgtac gcgcactgat ctttgatgca
    10681 cattatgatt cgtataaagg ggtcatcgct tatatccgtg tgtttgaggg caagatcaag
    10741 gcgactgatg tgctgcgcat gttctccacc caaactgatt tacgccctgt tgagatcggt
    10801 atctttgccc ccggtatgaa gcctgtggaa aacattggtt ccggcgaggt tggttacatc
    10861 gccaccggat tcaagaccgt gcatgaatgt cgtgtgggtg atacgatgac gcttgcttct
    10921 gcgcccgcct cagagccctt gcctgggtat ttcactccaa aacccatggt gtttgcgggt
    10981 atctaccctg tggaagccga tgattacacc accctgcgcg aatcgctgga aaaactgcaa
    11041 ctcaacgatg cttctttgag cttcctgcct gaaacatcgc aggcgcttgg cttcgggttc
    11101 cgtgcgggtt tccttggtct ctttcacatg gagatcattc aggaacgcat cgaacgtgaa
    11161 tacgatctgg atgtcctctt taccgcgcca tccgtggaat acgaagtgtt gaccattggc
    11221 gatgaagtgg tgaaagtgga ttcacccgcc gaattgcccg accccagcaa aattgtcgaa
    11281 gtgcgcgaac cgtggatgaa tattgagatc atcacaccca ccgattacta cggccccatc
    11341 atggacctgg tgaccaagcg ccgcggcatt ttcaaaaaac aggaatatcc cgcacctcac
    11401 cgtgtgcaac ttgatttcga gatcccgctt tccgagatca ttgtggattt cttcgaccat
    11461 ttgaaatcgc gcaccaaagg ctatgcctcg ctcgattatc aattcctcga ataccgcgcc
    11521 gataaacttc aaaaattgga gatccttgtc aatggcgata cagtggatgc gcttgctgcc
    11581 atcgtccatg aaaaggatgc ctatcacaag ggccagcgcc ttattaccaa actcaaagac
    11641 ctgattccgc gccagttgta tgatgtggcc gtgcaagccg cctccggtgg acgtatcatc
    11701 agccgggcca acgtcaaagc cacgcgtaaa gacgtgctgg caaagtgtta tggcggcgat
    11761 atctcgcgca agaagaaact gcttgagaaa caaaagcgag gcaagaaacg tctcaagatg
    11821 gttggtaacg tggagattcc gcaggaagcc ttcatggccg tgttgaaact ggatgatgaa
    11881 tagcctactg gatagttgcc agtaaatcgt aaatcgaaat gtcccaattc ctcaaagatt
    11941 ccaaacgctg gctgcctggt gccctcatca gtgtggcgtt gatcgctgcg attctgtact
    12001 tcgtggatat caatgcaatg gtgaacgcca ttcgttcggc aaattactac attctggcac
    12061 tggccatgtc tctctctttt gtatggatgc ttggccgtgc aaaggtttgg cagacgcttt
    12121 tgcgtgataa acccgcattt cgcgatgttc tttttgcaac cggggaaggc tacctgctca
    12181 ataattttct ccccttccgc ctcggtgaga tcggacgtgt ctttttgctc agccgcaaat
    12241 cggggttgca gtttggcgaa gtgctcccca cggttttcat cgaacgctcc gtagacctgg
    12301 cattttccgc ggctgtattt ttagccgcgt tgccctttgt ggctggcact gcaggctctg
    12361 aacgcatcgg tattatcgtt ggtattgcag tggtcattgg tttgttggtg atgtattttg
    12421 tggctcgcaa caaccaatgg gcgttgggtg tctttcacaa attgagcgcg cgctggccgt
    12481 ctttgcagaa attcggcggg gaatttcttg aatcattttt caccggcatg ggtgtgttga
    12541 cggacggctg gttgtttctg cgttttctat tttggatgac cctcgactgg gcgatcgcct
    12601 tgctgaatta ttacctgatg ctgcgggcgt tctttccgca ggcagaatgg atctggggat
    12661 ttttcatcct tggtgcggcg gcgtttggcg gcgcgatccc ttctctgcct ggggcagtgg
    12721 gtacatttga cggcgccatc gttggggcat tgaccctgct ctccggcgac caatccactg
    12781 cattggcggc ggcattgact gcgcgttttt acaactacct gaacagtggt gtgatcggag
    12841 gcattggcct catgcgcgaa ggtcaaacat tgacaggcgt ttatcaacag atcaaagatt
    12901 tcaggaagaa aaaagaagaa tgaaaaagaa ctatcttgtt accggcggtg cggggtttat
    12961 cggctcgaat tatgttcacc gattgttgca gcgcggcgaa accgtcacca tcttcgataa
    13021 tctttcgcgt gcaggcgcgc cgcgcaacct ggaatggttg aaaaaagaat ttggtgcgaa
    13081 tgcctttgaa gtggttgtag gggatgtgcg cgatgccgat agaatcgctg aagcagcccg
    13141 cagcgcggat gtgatcgtac atctggccgg gcaggtggca gtcaccacct ccgttaccaa
    13201 tccgcgtaat gattttgaag tcaatgcatt gggcaccttc aatgtattgg aagccgcacg
    13261 ttcatcgggc aggaacccga tctttttgta cgcctccacc aacaaagtgt atggcggcat
    13321 ggacgatgtg gaagtggtgg aggatgccac gcgttggcac tacaaagatt taacctacgg
    13381 tgcgcccgaa acccagccat tggatttcca ttcgccttac ggatgctcaa aaggcacagg
    13441 cgatcaatac gtgcgcgact atttccgcat ctatgacttg ccgacagttg tcctgcgaca
    13501 aagctgcatt tacggcccgc ggcagtttgg cattgaagac cagggctggc tcgcgtggat
    13561 gatcatcgcc gccgtgacag gacgccagat caccatctac ggggatggca aacaggtgcg
    13621 cgatgttttg catgtggaag atttgatgga tgcctatgat gcagcggttg ccaatatcca
    13681 cgttgcgaag ggacaggtct acaacatggg cggcggtgtg aacaacgtgc tggcgatttg
    13741 ggccgaattt ggcccgatga tcgagaacct gcttgggaaa aaggtcgaag tggctcgcgg
    13801 cgactggcgc cccggtgacc aacgtgtttt ttatgccgat tatcgcaaag cggagcgcga
    13861 gttgggttgg aagccgaaga tcagccttga ggaaggcatt gagcgtttgt tccaatgggt
    13921 gaaggaaaac aaggacctgt tctaggcagc catctgaaag agtttgtaaa gttttccgct
    13981 gctcaagccg ccgagaacat attttgaaaa atgtttttga aacttccgaa aggctcactt
    14041 tcggaagttt tattttcatc gcgttataat ctggctctct ggaattgata tcagcatctg
    14101 cgtaaccggc agatagccca ttggagtttt tatgcaggaa tatttttcgg aacttgccgc
    14161 tcagttcgtc gcttccattc caaatattgt taccgcgttg ttgatcttta tcctcagcct
    14221 gtattttgcc aggctgttga gtaattttct gaaacgggtg cttgtgcgcc gaaagaccga
    14281 ctctggcgtg acacagcttc tcacccaaac cctgcaatgg acgatcattg tgtttggtgc
    14341 gatcactgcg ttgcagcgat ttttcaatgt cactgctttt ttgaccgggt tggggatcat
    14401 cggttttacg gttggctttg cccttcaaaa tgtgatgcag aattttgtgt cgggcatcat
    14461 tcttttgatg cagcagccgt tcaaagtggg cgaggagatt cgcgtgctgg attttgacgg
    14521 tgtggttttg cagatcaacc tgcgcaccac cgaattgcat gcgcttgatg gccgcatcgt
    14581 catcctgccc aatgcagatg tgatttctca tgccattgtc aattacaccc gcgctaaccg
    14641 ccgccgcgtg gacctgccgc tcacgatcgc cagtactgcc aacccggaaa aggttcgttc
    14701 cctgttgttg gaagtgttca aggatacgcc gggctatgtc attgcgcctg caccggagat
    14761 caattttacg agtttcaacg atacctccat tgggttgacc gcttcatttt gggtggatac
    14821 agcagtgttg aaacccgaac atgccgccga tgccgccctg gtattggtga agaatgcctt
    14881 tgaaaaagaa agagtcgcaa gcccgctgcc tgcccaaacg gtttttctaa aatcggagca
    14941 gtagcagtta ttttgagctg attcgctggt gcagcgtgat tcaccccaaa cgtaaggaat
    15001 cagtcgcgaa tattttcaaa agtctgaaag attaaccacg gagacacaga gaaaacaatt
    15061 aaaaactccg tccgcaggag gctccgcact gcctcggtgt ctccgtggtt caaatgaatt
    15121 gcaagatacg gacgagttat aaaaagccga ccctgtaaaa tggatcggct tttttcgtgc
    15181 aaattcgcgg ctaaagaatt gaaagattaa tttcgggata tttcatgttt gttttcatgc
    15241 ttggatgtaa aaacggctac ggcagtttga aaatctccca gccgccaccg gattgattga
    15301 caatgacgat catgcctttt ccgtccagtg cgaggccggt gggacggctg aaatacccgt
    15361 catttcggtc ttgatcggtc cacgaccaga gcaattcgcc gtcgttggtg aatgcctgaa
    15421 tttgaccaag gtcataatcg ctgatgtaga cctgcccctg aacatccact acgatgcctt
    15481 caggggtgcc cttttttcca tctgcattgc ggatatccca tgttgtaatg aaacttccat
    15541 cggcgctgaa tttctgcacg ttcttatttt cgtaatcggc gacgaagata tttccattcc
    15601 cgtccacggc tatgccggtg gcatggctga atttgcctgt tgtggagcca gctccgcccc
    15661 atttcatcag gaagtttccg tcgttgtcga atttctgcac aaagggattt ccgtcgtcgg
    15721 tgatgtacac attcccatct ttatccacgg cgatgccaat ggcgcggaca aattgaccat
    15781 cgcccgtccc ttccgtgccc caggcggtga ggaagttgcc gtcgccgtca aatttttgaa
    15841 tgcggtgatt cccgttatcc accacaaaga catttcccga agggtccaca gccagcccgc
    15901 caaaacccat tgaattgaac tcgccgtcgc cgcttccttt cttgtcccat gcggcgagca
    15961 gggttccgct cgagtcgaat ttcagcaccc gtgaattgcc tgcatcagag atgtaaattt
    16021 cgccagcctg gttgaccgcg gcaccatagg gtgatttcag gtacaggtct cctgctttgt
    16081 ttacggtgag aaaaggccca attcccagat cagctgtcgg tgagggtgga atcggggttt
    16141 cagtcggtac gggaggttcg gtggggctgg cacatgcacc aagcgtgaat agggttgcca
    16201 ggatcaaaat cttcaatgca gagttcattt ttttctcctt gaataaagtc tttgcttgtt
    16261 catctttctt atccgttata atcttctggc gttatgaaaa ttctaaccgt acttacttac
    16321 taccgtccac atacttcagg actgactatt tatgccgagc gccttgcacg tgcctttgtc
    16381 aaacgcggcc atgaagtgac cgtgatgacc acccactacg atccatccct gccgcttgaa
    16441 gagatgatgg atggggtgaa ggtgattcgc attcccgtga tcgcgcgtgt cagcaaaggc
    16501 gtgatcgctc ccacatttgg gctggttgcc acgaaactgg tctggcagca tgatgtagtg
    16561 cagctgcatc ttccacagtt cgatgcgccc ggcgttgcca tgcgtgcgcg tttgttcgga
    16621 aaacccgttg tgctgaccta tcattgcgat gtgcaactcc cgcagggatg gtttaatcgc
    16681 ttcgttaatt ccgtgctcga ttttcaaaac gatatggcgg gacgccttgc gaatcacatc
    16741 gtgacctaca cacaggacta cgccgatcat tctccctacc tctcgcgcta cgcttcaaaa
    16801 ctgaccccga tcctgccgcc cgttgagatg cctgtccctg cgccggaggc tgttaccgcc
    16861 ttcgctgaaa agcaccgcac gaaggaactt aaacctgtca tcggcatggt cacacgcttt
    16921 gcctctgaaa aaggcgtgga aattttgctc gatgccctgc ccattatttt gaaaaaatat
    16981 ccaaacgcgc aggtcttgta cgcgggtcaa caccagaatg tgatggggga acaggcttat
    17041 tttgaccgcc tgattccaaa gatccatgaa tatgaaacct ccggtcattg gacctttctc
    17101 ggcaatctga cacccgttca actggcggcg ctctatccaa atcttgatgt gctcaccgta
    17161 ccatcgctca attccacgga ggcctttggc cttgtgcaga tcgaagcgat gatgaacggc
    17221 gttcccagtg ttccctccgc tttgccgggg gtgcgcagac ccgttcaaat gcacggcatg
    17281 ggcgtcgttt cagagatcgg gaacgcacaa agcctggccg cttccattct tgaagtgctg
    17341 gatacccctg caaaatatcg cggcgatgtg gatgggatca agaccaccta cgatcccgac
    17401 tcggtcgcgc aggaatatga aaaaatgttt gagcgtttga agggaagaac tacgggatga
    17461 tttcaaatcc gtgttggcgg aaatgatcat cgaaagtgaa agcggtgcgg atgttgtggc
    17521 gtttcatggt ggcaaaactt gagtaatcca ccatgctgat ctggcgacga ttccccgtaa
    17581 taacctgatc cataacccca ttgtgaagtg actcgtccag ccactcaatt tccaaaaatg
    17641 gaatgatgtc attgtgtaaa atggacagtg cttcaagtcc aacacggttc tggataagag
    17701 cgatgctttc cactaaaaca taattgttgc tggccagtct ttcgcctttt tcaatgaggt
    17761 ttttccaggt gtccagtgca atttgatacg atgggtcttc tggattgcaa atggcaagaa
    17821 aggcagatgt atccacatag atcatgagta agtctcattg aggtatttat cgtggttttt
    17881 ggccacgtct gtttttcctt caatatcttt gaagtgggcc ttcttgattt tttcaagacc
    17941 ttccaatgcg cggcggcgtt tttcgtccat ttgcgggttt actttggaag catcaacata
    18001 caaggttacg ctttcacgaa ccaatgaggc aacagatgct ttgcggaccc gtgccatttt
    18061 tttcaaggtt tttacctgtt cttctgttag ttgaaccatt acccgtacca taaacgcctc
    18121 tgctatcact ttttggaaag aatgctatca ttatactatg tcgcaaaaag actttctctt
    18181 tctccaagtc agttcgctgc cgtattttcg cgcatttctg cgcgcggttg aatcttccta
    18241 ctatcaagac ctgcccctcc ctgccccagt ctatgacgtg ggttgcggtg acggtcattt
    18301 tgcctccctg accttcgacc aaaaactgga tgtagggctc gatccgtggc gtttttccat
    18361 gcgggaggcg aaaaaataca atgcctacaa atccctcgtc gaagcagatg ggtcgcgcgc
    18421 gccgttccct tcgaaccatt tcgcaagcgg attaagcaac tcggttctgg aacatattcc
    18481 gcacattgat gcggtgctgg cggaaaccgc ccgcgtactg aaacctggtg cgccgtttta
    18541 tttttgtgtg ccaaacaccc gttatttttc cgcgctttcg ttgacgaagg tcttcggcaa
    18601 gccgtatgaa aactggttcc gcaagatctc ccgcgtccac catgccgacg aacctgacgt
    18661 atgggaaaaa cgcctggaag cagcagggtt caaactcgag cgttggtggc attacttttc
    18721 acccgcttcc atgcgcgtgt tagaatgggg ccactatttt ggcctgccct cgctggtggc
    18781 aaaagcgctt accggccaat ggattctttc ccccacaaag tggaatctca gcctcacgga
    18841 aaactatctc cgcaaatacg cctctcccga acccgtggaa gatggaacct tcacttttta
    18901 tattgcaaga aagaagtaat tggcaactca caattaccaa ttaccaatta ccaattaccn
    18961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc aattaccaat taccaattac
    19081 caattacccc catgtccttc gacgaaaaat acttctcaac ccatacctac gccaatatca
    19141 cctttgccaa atacagcatg tactggtggt cgaaccgatt ctatgccatg cttgcgcggc
    19201 ggtacgggcg gcgcggagcg cgtctgcttg aggtgggatc aggcatgggg catttggtgg
    19261 gccagctttc ggcgtggttc gaagcgtatg gcatggacct gaatcactgg gcggttaaca
    19321 aatccaaagc cgatgccgaa gccgcatccc tgcaaactgc atcagcacag gacctgccat
    19381 atcaaaacgg cgcattcaac gtgatcatca tcaagcatat tgtggagcat ttgcccgacc
    19441 ctgcaaaagc catccgtgaa attgcgcgcg ttaccgaaaa aggcggcatt ctcatcctgg
    19501 ccacaccgaa ccttggttca ctgctcaaac cgtggaaggg caaaacatgg atcggttatc
    19561 aggaccccac ccacatttcg ctcaaacaac ccgaagaatg gctcgagatc attcgcggcg
    19621 caggctttga aacgctccgc attttttcag atggcttttg ggatgtgcca tacattcgtt
    19681 atgttccaaa acagattcaa aagttattat tcggttccct tggcggtctg caagccatta
    19741 ccggattcgt ctttctgccg atgcgctggg gagaaagcat cattgtgatc gcgcgcaaac
    19801 gctaatttcc ttttgggtga agatgtatga atgaaccttc tgttctcgat tttgtaaaag
    19861 ccattttcaa aaactggaat tcttttcaat cctttcttca ggccctgttc aagcgggggg
    19921 atgcctctca attaatgcag tccgcagacg aagcgacgat tcaacttgac ccgcagcctg
    19981 atcctgaacc tgtggaagcc cccgccccga agaaactccc ctggcggaca ttgctcgtgc
    20041 tgttcctggc actcgcggcg caacagacct ttgagccgcc ccgcaccagc acagccaacc
    20101 tgggcgtcgc gctgtacatt gccgcctttg ggctggccgt gtgggcctac ctgcgcggcg
    20161 aatggacgat gactcccgtt tctcagatcg tgcccggtgt ggaagatgcc agcatccgcc
    20221 gcggctcgtt cgtgctttcg gtcatcttca gcgccgcaac cttttggatg atgggcggca
    20281 ataaattcac tgccctgaat ctcacactgt gggtgcttgc cattgtgttt ttcctgcgtg
    20341 ctttctggct taatgaaccg cgcagcgccg catcgtcaaa cttcgatctg aaatccatct
    20401 tcaccagcag cgaatggaat atccgcatca cacgcagtgc tttggccgtt tttgtgatgg
    20461 cggtcatcat catcttcttc cgcacgtatc gcctgtacga tgtgccccct gaaatgacca
    20521 gtgaccacgc cgaaaaattg atggatgtgt acgacatcac gcagggacag ttctccattt
    20581 attttccgcg caacaccggg cgtgagccgc tgtatattta tctctgcgcc ttgttcgcag
    20641 agttgtttac gggtacctcc ttcctcactt tgaaagtggt ggcagtcatc ggcggtctgc
    20701 tgaccctgcc ctacatttac ctgcttggaa aggaattcgg cagcgaacgc attggtttgc
    20761 tggctgtgct gtttgcaggc atcggatatt ggcccagtgt gatcgaacgc tttggcctgc
    20821 gcatttcttt ctacccgttg tttgctgcgc caactttgta ttacatcatc cgtgggttgc
    20881 gccgccagaa ccgaaacgat ttcattcttg caggcattgc cttgggcgtc ggtttgaacg
    20941 gctacacccc attccgcatc atgccatttg tggtggtggc cttgttcggc gtgtacctgc
    21001 tgcatgcccg tgactggcag gcccgcaaac aatccatcat ctggctggct ttgctggcct
    21061 tcacttcttt tgttttcttc atcccgctgg cccgttacat gatcgagttc ccggacatgt
    21121 tcgtgtaccg tgccttttca aggttgagca cggtggaacg tccactgcct gcgccgatct
    21181 ggcaattgat ccccttgaat ttgtggaatg ctgcaaagga attcaactgg tataacggcg
    21241 atatctgggt gcattccatt cccggtcgcc ccgcattgga cattgtctcc ggtgcgctgt
    21301 tcctgctggg tgtcagtctg atgcttttcc gctacatccg ctcacgcagt tggatggacc
    21361 tcatgctcct gctggcggtt ccattcttcc agatgccttc cattctttcg ttggcttttc
    21421 cagaggaaaa cccgtccttg aatcgaacgg gggctgcgat cgtgccggtc ttcctgctgg
    21481 ttgccatcgg gttggatacg ttgttgaaca gtatcaacct gcgggaaaaa gcgaacgaag
    21541 aatccatcat gagcgaaaat cgccgcgcaa tgggacatcg tccgcttttg gcgggaacca
    21601 tcttcgtttt gttattcctc ttttccttta ctcaaaatta caaccttgtc ttccatactt
    21661 attacgacca ataccgtgct tctgcgtgga attcatctga aatgggtgcg gtgatgaagg
    21721 gtttcatgga gcgcggtggt tctgccgaca atgtgtggat cgtgcctttc ccattctggg
    21781 tggatacgcg tctgccgccg ttttgggcgg gtgtgcccgg cagggatatt gccattgccc
    21841 cggataacat cccagccacg gtggagcttt caggtgacaa gctgttcatg ttcacactgg
    21901 atgacacggt gaccatgtct gaattaaagc gtttgtatcc gcagggcaca ttatctcaat
    21961 ttcattcggc agttcccgcg cacgattttt acgtgtatca gatccctgcc tcgcaataac
    22021 ccgcagactc caatgtcgga aggcattgga gtctgttttt ttaatcatgc aaaacgaacc
    22081 atcccatctt aaagcatttg gttttccgtg gcggtctctg gcagcgcttg tgctggcatt
    22141 gaccgctcaa ctcatgttgg aaccgcctgc aaagatctgg gtgggcatcg tgctttacat
    22201 tgccgcgatt gggctggtcg tatggtcgtt tctgcnnnnn nnnnnnnnnn nnnnnnnnnn
    22261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22321 nnnnnnnnnn nnnnngtaca gaaaatggat tgggaatggc tcgtgctgct gcttgctgtt
    22381 tttgtcttcg ccgccttctt tcggttttat cgtttgaacg aagtactggc cgagccattt
    22441 agtgaccatg ccgaaaaact gctcgatgtg tacgacattt cgaaaggcaa cacgtccatt
    22501 tttttcatcc gcaacaccgg gcgtgaaggc ctgcaaatgt attggacctt tcttgttgca
    22561 aaaatattcg gcacgggcat ttcgtttttt agtttaaaac tcggcacagc cctgatcggc
    22621 ctgttcacac ttccgttcgt ttatttgctt ggcaaagagt ttggaaaccc cgtggtcggt
    22681 ttttttgcca tgtttttatt tgggatttcc tactggatga acgtgaccgc ccgcatcgga
    22741 ttgagatttc ccctgtatcc gctcttcgcc gcgggcacgc ttctttttct aacccgtggc
    22801 ctgcggactg cttcgaggaa cgattttctg ctctgcggcc ttttgctcgg actcggcctg
    22861 cacggttaca ccccgttccg catcatgccg ttcgtggtgt tgaccgcgtt cgcgattttt
    22921 attttgcaca acaattcaaa ggcgattcgg gtgcaggcgg ttcaaggcct gacgatcatc
    22981 gccgtcaccg cgctgatggt tttcctgccg cttttgcgct attggctgga ataccccgag
    23041 gtgttcggct tgcgtgcctt ttcacggctc gactcggcgg gaacttttgg ttcgctgatg
    23101 ggcgtcttct tttcaaattt ggccaacgcc ctgctcatgt tcaatgtgga tgatgggaac
    23161 atctgggtga attccctgcc gcaccgcccc gcgttggatg tggtgacagg cgcatttttt
    23221 gtgatcggtc tggtgttgtt gggtttgcgg tatctgcgcc aacgggattg gcgtgacctg
    23281 ttcctgctgg ttttgattcc gctgttgctg atgccttcgg tgctttcgct ggcgtacccg
    23341 gaagagaacc ctgctttgaa tcgtgcgggc ggcgcggcag tggctgcgac tttgattgcg
    23401 gccctggcgg tggaaggcct ggttgtagga actgtccgtg gtgttgaatc aggtcagggt
    23461 ccagaaaaga ggcggggttt catcctccat attttggtgg gagtgttgct ggcggctgcg
    23521 tcttttcaga attacgacct tgtcctgcgg caatttgacc gctatttccg cattggcgcc
    23581 tggaattctt ctgaaatggg gcgctcgatc aagttttttg gcgatgtgta tgggcgcacc
    23641 gatacagcct ggatcgtgcc ctacgagcag tgggtggata cccgcctgcc cgccctgtgg
    23701 atgggtgacc ccaaccgcga ttttgcgctg tggccgagcc agtttgccga tacgctggca
    23761 atgcctgcgc cgaaaatgtt catcttcaat cttcgcgatc tggacactaa aaatgccctt
    23821 aagcaactct acccgaatgg tacgttaagc agatatactt ctgccacgac cggcaaggat
    23881 tacatgattt tctttgtgga ggaatgatgt cgttgtgagt tggaggccgt gcggactccc
    23941 tcttttcact gacatgaagt tttatgactg aaaaacgcgc atggttttat gatcttcttt
    24001 ttgtctgtat cctgctgatc gcagggtttt tgcgattgac cggggtggag tggggagagg
    24061 gttaccacca gcaccccgac gaactgtttt tggtgggtgt gctcgataac ctgcgggcgc
    24121 acacctgcgc cgatgccgta accccggtgg acctgtgcaa acccgaagac caacgctgga
    24181 tgacccttgg ggaatatttc gatacggcca cttccaccct cagcccctac aaccgcggac
    24241 atgccttctt cgtctatggc aacctgccga tgacggtgac ccgcattgcc atggaaatga
    24301 ccggcaacga tgacattggc aaatcgaagt tctttgcgcg ccaattttcg gcgctggccg
    24361 acctgttcac catttttatt ttgtatttga tcgtttcaaa tttatatggg cggcgtattg
    24421 gtctgtttgc ggctgtattt tccacattcg ccgtgatgca gatccagcag tcgcatttct
    24481 ttaccaccga cctgttcgtg aacctgttca tgttcctggc gatcatgttt gccatgggca
    24541 ttgttgaaaa acgggatttg ggcatcggca acaacgaatc ggagactttg caattaacgt
    24601 catctgatgc cgggttttca aattacctgt tgcgtttggt gcgctcgccc cttttcctgc
    24661 tcagcattgg ttttgggttc gcgcttggca tggcgatggc ttccaagatc aatgcggcgg
    24721 cgctggcgat cctgctgccg ggggcatttg ctgtccgttt tctggtgcag gacaaagaca
    24781 aaaaactggc cgctgattat tggtcgttga tcactgtctt tttgatcgca ggcggactcg
    24841 ccaccatcat ttccttccgt attttccagc cctatgcctt caatgggctg gggttgaacg
    24901 atcagtggct ctcgaacatt gctgaacagc gcgtgcaagc caatggcgat gccgacctgc
    24961 cctggaactt gcagtgggca cgccgcacac atctgtattc cttcaccaat ctcaccgtgt
    25021 ggggattggg tcttccgctt ggcattctgg catgggtggg tttcctgctg atgggttggc
    25081 gcatcctgaa aggcgagtgg cggcacctgc tgttgtgggg gtggaccgca ttctacttcc
    25141 tctggcaatc gctgcaattc aatgccacca tgcgttatca actgccgatc tatccgctcc
    25201 tgtgcatgat ggccgcctgg gctttgtttg aattgccgaa attgcgcggc gaaaccgggc
    25261 gttttaaatt attcgaagtc ttggtcgcca ccatgggtgt ggtggtgttg gtgacaacga
    25321 ccatctgggc ctttgccttc caaagcattt acacccgcga cgaaccacgc atggccgcct
    25381 cgcgctggct gttccaaaat gcgccgggcc cggttaatgt gaagatcaaa atggccgaca
    25441 acaccatcta caaccagcca ttgcctgtgc ccatggatat ggcgattcag gcaggggcgc
    25501 ccttcacaat gaccttttcc gccaatcgtg atggggatat ctctgaaatc gtctttggtc
    25561 atgccattga tcttgaatcg ccttcgacga ttgtcctgac cctggcatcc gcctctgcac
    25621 cggacctcgt gctggcgcgg gcctccgaaa aagtggatta ctcgcagacg aaggatccgc
    25681 gcggccccgc cgtggaattt aaactggatg agaccgtccc gcttgtcaaa ggtcaggcct
    25741 atcttttgaa gatcgaaacg ctcggacatg cgctcacttt gagcggatcg gccattgcca
    25801 acgaaacagg ttacgactgg ggtctgccct tccgcattga cgcctacgat gccttcggcg
    25861 gcatctaccg cggcgacctg accctcgaag tatattggga tgacaacccc gataaggtca
    25921 accgctatgt gagcatgttg agcatggccg attacatcgt cattcccacc aatcatcaat
    25981 atgcacagat cacccgcctg ccggagcgct atccgctgac gacctattac taccgtaaat
    26041 tgcttggctg ccccgaaaac gccgatatca tcgaatgcta tcgcaacgcc aaacccggtc
    26101 agtatgaagg tggtctcggt tttgaactgg tgaaggtttt tgaaacctac ccccagtttg
    26161 ggccgatcat catcaacgat caggcagcag aggaagcctt tactttttac gaccatccaa
    26221 aggtgttgat cttcaaaaag acctccgatt tcaacgcgga taaactatct gcaatgctca
    26281 acaccgtgga cctgacaaac gtagtccacc tcacgccgaa gggcgccgat aactacaaga
    26341 gcctgatgct ttcgaaagag cagtacgcgg cacagcgtgc gggcggcaca tggtcgcagt
    26401 tgttcaatta tgaatggctt caaaacaaat accccgtgat cgggcttttg ctttggtatg
    26461 cattcatctt cctgcttggg cttgcggcct atcccattgt gcggctggct ttgccgggct
    26521 tccgccaata ttcatatccg ctgggacgca tggcgggttt gtctttgctg gcatggattt
    26581 cgtggatggg cggttcgctg ggcgtgccgt acacacgcgg caccatcggg gcggcctttg
    26641 tccttgtcac tgtggcaggc ctcgggttgt ggtggattcg caaagcgcag ttcaaagatg
    26701 agtgggattc caatcgcaag ttcttcacgt tggtggagat cgttttcgct gtcttcttcc
    26761 tgatcgatct gctcatccgc ttcggcaact ctgacctttg gcatccctca aaaggcggcg
    26821 agcgcccgat ggatttctcg tatttcaacg ccattttgaa agggacgagt tttccgccgt
    26881 atgacccgtg gttcgcgggc gggtatatca attactacta ttatggtttt gtgctggcgg
    26941 gcacgccggt gaaattgctc ggcattgtgc catccattgc ttataacttt attttgccca
    27001 cctggtttgc gctggtggct gtgggtgcgt ttgccatcgg ttggaatttg ctggaaaaag
    27061 aggaaggtac ttctcccaac ttctccgcac agcattcatc cttgctttcc ggtctttctg
    27121 ctgcggttct gtctgttttg cttggcaacc ttggcacgat ccagatgatc tttcaggctt
    27181 tgcagcgtat ggcggcaccg ggtgggatca ttaccgatgc cacctttgtg cagagatgga
    27241 tctgggcttt tcagggattc atgatgtcat ttggcccggg cgcttcactg ccgatcgggc
    27301 gcggagaatg gtattggaat cccagccgcg tgattccgcc gggcccgggc aatgagatca
    27361 ccgaatttcc gcttttcacc tttttgtaca gcgatttaca cgcgcacatg ctggtgatgc
    27421 cgctggcatt gtttgtcatt gcgtgggcgc tgtcctttat caaatcgcgc gcgaacctgc
    27481 gccgtgaaga atgggctgct tcgttcctgt tgggcgccat ggtgctggga gcggtatacc
    27541 caaccaacct ttctgatttt tatacctatt tgctgttggc gttggtcgtg cttgcgttcg
    27601 tcttcctctt ccgcgctgaa gtgagcggac tgcggttgct ggaagacctg cccgatgcag
    27661 tgaaacgcat cgccttgatg ctggtgagca tcggtgcatt gaccgtgctt tccttcgtca
    27721 tgtatcaacc gtatcgcagc gcatacagtc aatcgtatgg cgcactcgac ccgtggacgg
    27781 cttcgcatac acccgtttct tcgtacctca cccattgggg attgttcctc tttatcatct
    27841 tcgcatggat ggcttgggaa acccgcgaat ggatggcgcg cacgccgctt tcttcgctgg
    27901 caagcctgcg gccgttcgca attttgatcg aacttgcgtt ggcgggtatt ttcgtcgttc
    27961 tggtttactt tgcctacgaa gggatctggg tcggttggct ggcattgccg ctggcagcct
    28021 gggcaggcgt tttgctcctg cgccccgatc aacccgacga aaaaaggctg gtcctgttcc
    28081 tcattggcac ggcattgatg atcactcttg tagtggaatt gcgaaccgtg cgcggcgaca
    28141 ttggccgcat gaacacggtc ttcaagtttt acatgcaggc ctggctcatg ctttcggtca
    28201 gtgcggcggc ggccctcggt tggatgttta gcgatgcccc gcgctggtcc gctggatggc
    28261 gcaacaccgt gcaggtgatt cttttgacgc tggtctttgg cgccaccctg ttcaccttca
    28321 ccgccacctc agacaaggtg acagaccgcg cctcgaccga tacgccgcgt tcgcttgatg
    28381 gcttgaacta catgaattac tccacctact tcgatcacgg tcaaaacctg accctgaacg
    28441 aagattaccg cgccatccgc tggatgcagg acaacgtgga aggctcgccc gtcatcgtgg
    28501 aagccgcacc tgcaggcagg caatacgaat ggcattcacg cttcaccatt tacacaggcc
    28561 tgcccggtgt ggtcggctgg caatggcacc aacaacagca gcgcgtgttg ttttcgcagc
    28621 agatcattga ccgcggcatc gaagtggatg ccttctacaa ctcgctcgat caagactctg
    28681 tgcgaaaatt ccttgcgaaa tatgaggcgc gctacatcat cgtcggacag ttggaacagg
    28741 cgaagtacat gcccattgac ccgggcattc caaacggcct gatgaaattt gacctgtgga
    28801 atggcacact ctggaatgaa gtctatcgcg acggcgacac cgtcatttac gaggtgatcc
    28861 aatgatcctg cacatcacat cccgtgctga atggagcgcg gcgcaggcac gcggcgaata
    28921 tattgcgccc agcctgcaga ccgaggggtt cattcattgt tccactgaca aacaggttgc
    28981 ccatgtggcg aatgcctttt atcgcggacg aaatgacctg gtgttgttaa tgatcgacga
    29041 agcaaagctc aagcccgagc ttaagtggga accgcctgct ggaccacccg ccccgggcat
    29101 ttccgaatcc gattcatttc cacacatctt tgggcccatc aacttaaccg cagtcgcctc
    29161 tgtgctggac tttgaacctg atcctgcatc tggaacattc tcctacccca aggcatgacc
    29221 gaaccagccg ccgcccttcg tattcgcccg gcccatgccg ccgattccgc gcttgctgca
    29281 aaggtgattt acatgtccat gggcggcctt gccgattatc ttttcgataa caacgggcag
    29341 gcgatccaga cgatattggc agagttgttc acacgcgatg ccgggcggtt tggttttcgc
    29401 atcgccatgg tcgccgaaga tgaaacacag ccagtgggca tgctgttcgc ctgtcctggc
    29461 gcacggctgg cgggtttcaa tatggcttcg tttcagcatt tcttcccggt catggggttc
    29521 aaacatgcgc taaaattcat gtggcgtggt gtaaccctgc ccggcggccc cgaagctgaa
    29581 aaggacgaat attacgtcag caatctcggc atcctgcccg aggcacaggg acgcggtatt
    29641 ggcacatctc tgctggctta tgccgaagag acctgccgtg ctcatcaact ctcgaagtgt
    29701 tcgctcatcg tgggtatgca taatgaaaat gcattccgcc tctatcaacg gctcggttac
    29761 caagttgtcg aaaccgctca tgcgcccaac gaacaccttg cctattaccg catggtcaag
    29821 cacctgaact gatcgcagac tctccacaca ttggatctca caaatgaccg catttatcgc
    29881 ctggtatttc atcctcaccc ttctcggctg gctgaccttt ccgctggctc attttctttt
    29941 ccccgcttta aaagatcgcg ggtacaccct ttcacgtgcc gccgggcttt tgatttgggg
    30001 gtattccttc tggctgatgg cctcccttgg tattgcacaa aacgatctgg gtggaattct
    30061 gttggcgttg atcgtgctgg cctcattgag tgcgtgggta ttggccaatc aaaggtctga
    30121 gatcattgat tttttcagaa cgcatcgttc actggtcatc accacagagg tcttgttcct
    30181 ggtcgctttt gcgttcctgg cttttgtccg ctcggccaac cctgaattga ccggcaccga
    30241 acggcccatg gaacttgcat tcatcaacgc cattctgcgt tcgcctgttt ttcctccaca
    30301 agacccctgg ctttccgggt acgcgatttc ctactactac tttggctatg tgatgaccgc
    30361 catgcttgca aaagtatcgg gcatttatgg cagcatggca cacaacctga tgaccgcgct
    30421 tgtctttgca ttgggagcga tcggctccta tggcattttg tacaacctgc tatcacatgg
    30481 acatgaaccg aagaagaccg aagacggcga tatggatacc gctggctcgc atcgttcttc
    30541 tttttccatt tatggttttc tatccctgct ggccccgctt tttcttttgc ttatctccaa
    30601 tctgggcgca ttgctggaag tgatgcatca atggggtctg ttctggaaac aggattacat
    30661 cagcggagaa tacaccagcg cattttggaa gtggatggat atgaaggagc tttcacagtc
    30721 tcccattttg ccattccatg cgccgccgct tgaccgatac ctgtggtggt ggcgttcttc
    30781 acgcgttttg caggattatg acatgctcaa ccgtccgctg gaggtgatcg acgaattccc
    30841 gttcttctcc ttcctgctgg gtgatttaca tccgcatgtg ctggcgattc cgttcggttt
    30901 gctggcgatt gcagcagctt taaatctctt tttaggcggc tggcgcggtg aagtcaaatt
    30961 accgttgggc gcacgtttgc agatcagcgg accggggttt ttattcacag ccttgttgct
    31021 tggcggattg gcctttctca acacatggga tattctcatt gccgcggctt tgatcgttgc
    31081 ttcgtatgtg ctttggtgtg caaatgaaga aggctggcga tgggaccgcc ttgaagatgc
    31141 gctgttgttt ggttttccgc tgggcattct ggcgattttg atgtacctgc cgttctatgt
    31201 cggcttttca tcgcaggcgg gcggcatttt acccaatttc atgtacccca cccgcggcat
    31261 tcatctgtgg gtgatgtggg gcacactgct tgttcccatt ctggcttatg tcttgtatct
    31321 gtggcgcggc gaaaaattcc ctgccaattg gaaactcggg gtttccctcg ggctcggatt
    31381 cgttgtgttc ttgctggtct tttcgtggct gttgggggtg atcggttcca tccttgaaaa
    31441 ggattttgtg aatggattcc tcgcctcaca gggaatgaca gccagtcagt tctttggcgc
    31501 tacactgatg cggcggttga gttacattgg cgggctcatc accatgcttg cggtttttat
    31561 tccctcactg gcttttctgt tttcgcaaaa gcaacaaggt cagcccgaag ccgaattatc
    31621 ggcaccattg gaggataatc gcaaaccaga gacccagttt gttttgctga tcctcatcct
    31681 tggcgccatt ttgatcatca cccccgattt cgtatacctg cgggacaagt tcggctatcg
    31741 tatcaatacg gtgttcaagt tctattatca agcgtggatg ttatggagtt tggcggcggc
    31801 ttttgctgct tcacgtttgt tgcaaagcct gcgaggcgcg gcgaatgtag ccttccgggt
    31861 ggcgctggtg ttggtggtct caacggggtt gttgtatcct gttttggggt tgatcaccaa
    31921 gacgaacaac ttcaacccgc cgtatggctt tgcccttgat gattttgaac gggtcatccg
    31981 tgagacaccg gaagaggcgg ctgcgatcaa ctttttacgc actgcgccgg atggcgtcgt
    32041 tgccgaggcg atcggcgatt cgtacaacta tccatttacg cgcatctcca ctcatactgg
    32101 cctgcagacg gtgcttggct ggcccggcca cgaagatcaa tggcggggca gttatattcc
    32161 acaaggctcc cgccgcgatg atatcaaccg tctttacacc acggtccgct gggaggaagc
    32221 cttgcagatc attgagcagt acaacatccg ctatattttc attggcacac tggagcggac
    32281 ctcgatgaat gtgagtgaag aaaaattcat tgcccacctt aaaccgatct ttcaacaagg
    32341 cggcacggtc atttacgaag tgccagtgct ggagaaataa aaatataaaa aaaagtcccc
    32401 gaactctcgg ggacttttta ctgtaattag ggttttgcaa cggagaattt ttctttgccg
    32461 atcttgtaat tgccgctgtg tttcagcacc tggtccacgg catcggcagg gacatctaca
    32521 aaagaaacct tttcttcaat gcgaatttta ccgatggtgt agccggggat gttggcatgg
    32581 aaggcgatct gccctacaat gtcatttggg cggatgttat gggccttgcc tttgttgatc
    32641 ttaaggcgca ccatgccttc ctcgtgtgag tcgcggcgtg attttttatc accgaattca
    32701 cgcttgctgc ctttggctgc agagcctgca ccaaactcgc gcttgccgcc ttttgaatca
    32761 cggcggccaa attcacgctc gggcttgcgg gtgaagtcgg ttttcacatc agacacttca
    32821 gcaatgggac gctgtttttc gtcggcgcgg gcgatcttaa tggcggcggc ggcgatgttc
    32881 attacgtcat gcccggcttc gatcagttcc tgcaccattt caagttcacg tttgtatcgc
    32941 gcacggccaa gccagatctt caggttttcg accaccagag attcgcggtg gcgcatgatc
    33001 tcagaggcgg tcggcagttc catttttgta acgagttgtt tggtgagcgc ttccacttca
    33061 cgcatgcggc gtttctcgcg cggagagatc aggctgatgg cgatgccggt tttgcctgca
    33121 cggcctgtac gcccaatgcg gtgaacgtac acttctgcat catcgggcag gtggaagttg
    33181 aagacatggg agatgtcgtc aatatccaac ccgcgtgcgg ccacatctgt agcgaccagc
    33241 accttcaatt gattggcgcg gaaacgcccg agcacacgct cgcgggcatt ctggtcaagg
    33301 tcaccatgaa tggcttcggc agggatgccg cggatgacca attcattggc gagttgtcct
    33361 gtttcggcgc gtgtgcgggc aaagatcaga gcgctgtgaa tgggttcgac ttcgaagagt
    33421 cttgtgaggg cgttgatttt gtcgttgtcg tggacaaggt agtagcgttg ttcaatggct
    33481 gcagcggtga gcgtgcttct tttgatgacg acagattgag ggtcacgcat gaagcgattt
    33541 gcaagggcgc ggatgcgcgg gggcatggtg gcgctaaaga gggcggtttg cctttcggag
    33601 ggagtcatcg aaagaatttt ttcgacttct tcaatgaaac ccatgttgag catttcatcc
    33661 gcttcatcca ggacgacggt cttgatctcg tttaagatga ggactccacg ctcaagcagg
    33721 tcgtttaggc ggcccggtgt accgacgacg atatcaacgc cgcgtttgag actgttgatc
    33781 tgcgggctgt acggttggcc gccatagacg gcaagtacgc gcaggttgag gtgtttgcca
    33841 tattcggtca tcgaatcggc cacctgcagg gcgagttcgc gggtgggggc gagtaccaat
    33901 gcctgcggat ttttttgatg ttgaaagttg ttcagaatgg ggagggcgaa ggcggctgtt
    33961 ttgcctgtgc cagtttgggc ttgaccgatc acgtcaacgc cggtgagcat gaggggaatc
    34021 atgcctgctt gaatgggggt gggctcggaa tagccaagct cagtgatggc ctgcgtgatc
    34081 tcttcacgga ggttgaggga ggaaaaatcg gttgtcatct ttgtccttta gatctgtggt
    34141 cggctggtcc atgatttgga tcggcgaccc taagggattg ctctgatgac tccgctgtaa
    34201 atttcttcct gtcaacaagg tttgcttttg caggcaaacc cgcccaacaa aaaagcccga
    34261 taaagtttca tcgggcattc gttcagagtg atcaaaacaa tttcccagaa aattcgcctt
    34321 ccattgaagg cgggaagagt ttaccacagt ttttgagatt caagccctga tacgcagagg
    34381 cgcgaacttt gctatacttt gtgtatggct gctaaaaatg ttcacctcaa ctggaatcct
    34441 gaccagcaat tcacccttca ggataatgat gggtaccagc tcgttatgaa aaagccaaag
    34501 ggagtcagtg cttctgacct gctgcccatg gccttgatcg gatgcgcttc gcacgatgta
    34561 gtggagatcc tcaataaaca gaagcaggat ctacgtcaat tgaatgtgac tgcggaaagt
    34621 attcaagatg agaccccacc atggcgtttt cgaaaaatcc acattcattt tcaggttgtc
    34681 ggcaaaggca ttgaccctga aaaggtgcat aaggcgattc aactgagcga ggaaaagtat
    34741 tgctcggtgt atgccacatt gaaagatgcg gtggagatcg tcaacgactt tgaagtggtg
    34801 gaagcgtaaa ttttacgggt ggtttgaaac aagtatttga aacagatgcc gctttctggc
    34861 ggtcgaatgc gttgagcggg atataaacag tttttgaatc agatgcgttc ggctagtttg
    34921 tctaatatag agatggttcc catccccagc aacatcagca ggccgccgat caggatcgga
    34981 atggatacgg cagccaataa tacggtggta aaaaaggtgt taccggtctg cggaagatac
    35041 cacaccgtgt ggtcctcaag aatggcatag tagatttttt gcgttgaacc gggataagca
    35101 ggaaccccga tcaggtttgt aaagacaccg ggtgcttcaa tgtcaggtga ggttgattca
    35161 tccatctctg gtagaagaga agcgtcgggc ttttcatctg ccagccatcc caaaccatcg
    35221 tctggaatgg tgtgcgaaaa ataattccct ttttctgttt caatgatgat gcggttgtaa
    35281 atcccgcccg aaatggttct ggcggcttcg ggtggatcgg gcaactgcac ccaactttct
    35341 ttgggtgaga aaaaggtata ggtgaggata agcccaagaa tgggcagaac aacacaaaga
    35401 aagagggagc cgaagatcac tccatttatt gccagtttgc gcttcgcaga atcctgaatg
    35461 cgaagaagta gacggttgat tcccactccc gaccagaaac acaacaaaat taaaacggcc
    35521 ttccaaagcc catcagccaa ccctagaaag aaaactcgaa actgaaatga cggttcgagt
    35581 tgttgaagcg catcttgttg aggttggagt gtgaaaaagc ggatgagggg cagcacaatc
    35641 attaaggctg cagagagtgt aattagcacc aaaaacggga agcgctgctt aagtttcata
    35701 aggcgcgatt atatggatgc tgtctttttt ttcactcccc tgttggtaca gtctttgaaa
    35761 acaaaattgc tcccacaaca ctcagcaccc ataatcatct gtcctcttat atcaaaaaca
    35821 ctgggacatc catcccagtg ttctctcgtt tatgcttggc tatttacttc tgcgtgcctt
    35881 tgccgcaatt gacctgacct tcttttgctt ttcaacaacc acctttgctc gttttcggat
    35941 aagggaacgc aaagccgcgt tgacactttt tgagtcaggg aaatgtgcct tcacatcctt
    36001 atccaacagg atggcacttt ctttggggac aaaatgccgc atggtgacag ttccgtcctc
    36061 atgcatcacg cggacagaat accctgcttg caaggctttg acatatttcc cgcgtacacc
    36121 ttttttgcct tccaaattat attcaggcag catatcttca gtttgagaag tcttattcgc
    36181 ctttttcata tcgttttctt tcggttggag ttgcttttcg acaactgatg atacggatga
    36241 tgatggtttc tttcttttct tcacgttccg tgtggactac aagcaaaata cgattgtttt
    36301 cggaaaggcc aatactgatg aagcgctctt ccttttcgga atgcagttca tccgcgaagg
    36361 tcacaagaag tgggtctaca aatattgtgc gtgcctcatc aaaactgact ttgtgcttct
    36421 caaaattggt acgcgctttg ttttcatccc actcgaatat gatcttcata cccatattat
    36481 acccgcgcct ctattccgca aatggactag cctttaggag agggtggggg gagaggccca
    36541 cccttcaaat caaaacacag ggacacccat ttaataatgt tttttggttt cccgtcaaac
    36601 tggttgacga tgtatcttag cttgatacct cttgaatact ttttcaagat gataaaatca
    36661 agtcgtttac tcggatttca aaaggacaaa taatggtaga catcttgcag aatatcgggt
    36721 cgactctatg tatattgggc ctttgctttg gccccttcct tttcattggg tttgggcttc
    36781 cagcgattgg gtacattagt aaacgcagga acgcccgcaa acaaaagcgg agcaaccagc
    36841 agcaccgctc aaggttccca tatcggcttg ttgcgtatgc tatgttgggg gtgaccctga
    36901 tgacagtggc catctgcatt tactggctct tttcagggac gttcgacatt tcgaacgatc
    36961 ttggcggggt tgcaggaatc atcgctgttc cgcttttgtt caccattgcc gccttccgtt
    37021 cctacattct acaaaacatg ccgtccgcga ccgaggcagt cgagcaggat ccacgactgc
    37081 ccgtgttata tctgcgttcc ttcgaccagg agatgctgaa attcgtacat ctaaaggatg
    37141 aagaaaaagc cagatataac accttcctca attacgccgg tgatgtcaac cttccgccct
    37201 ttgaacaagc gatctacacg gcattgaaca gttccgcatt attgaccttt ggctctgtgg
    37261 agtctgagga tgtgaccttc gaaaaatatt tccgcggcga ggtgatggaa cgcatcggac
    37321 ccttcgtggc gctgggcaac cccattgaca gcctgcctac cgagggagcc tttcgcgatt
    37381 accagatcga tgaacgttgg aaggatgttt tcttcaacca ttccgaacgc gccgcgtgca
    37441 tccttatgca attaggcagc tcggacaacc ttcaatatga gttgaccgcg atcctctccc
    37501 gtcatatttc gcataaattg ttcatcctaa ctccacccca ggaaaatctg aaagaccgtt
    37561 atgcctgggt acggaagtat atcctcaggg aaaagcctgc aaactggcag gcattctccg
    37621 ccatcctcca aaaaaatggg tttcggattc ccgacgaacc gcttgggatc gggtctgtgc
    37681 tgacctttga gaaggatggt tccccgctgg ttcttgtccg taacgctttt caaccgcagg
    37741 aatatattca gcccattgca gagaggttga agg
//
LOCUS       DAOQJC010000025        36607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_489_length_36607_cov_8.673397, whole genome shotgun sequence.
ACCESSION   DAOQJC010000025 DAOQJC010000000
VERSION     DAOQJC010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 36607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36607
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_489_length_36607_cov_8.673397"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="PLE39_05490"
     CDS             <1..879
                     /locus_tag="PLE39_05490"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE67811.1"
                     /translation="GGQIQILQTLMQVETYTRYLISYPSDGLTITGILQVPAGEGPFP
                     VIVMNHGYYNRGEYNSGDGSDRAAAYLVERGYITVSSDYRTWGGSDIGYSFFHTGLVA
                     DVMNLIASLPSIPQADATRVGMWGHSMGGGITTKILTLESPVRAAVLYAPNSADDADL
                     IARWGYGCIGDIAAGELLNTCNAADVIPVTLSDELIAAYVESATSAERMREIAPYYHL
                     ENISVPVQIHIGSADGDFIGATPPEWSYKLNQGLLDAGKDVELFVYEGQRHSFTGEAW
                     FTFMQRALDLFDEQLK"
     gene            884..1993
                     /locus_tag="PLE39_05495"
     CDS             884..1993
                     /locus_tag="PLE39_05495"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNE67812.1"
                     /translation="MKTKSMRFWVCLVLFALSACLAAPLSPPPVPAPVETATKIPVTF
                     TPSVTPSPTATRTTQQALYPFTIEGLRKHKFQSGEIIIRETLLETEVFTSYLIEYPSD
                     GLTITGVLQIPKTGEPPFPVIVMNHGFFSRYIYRSGDGTDRAAEFLNRRGYLTVSSDY
                     RSWASADTGESLFYSGLAIDVVNLMNALPSIPEADVTRIGMWGHSMGGGTTLKVLTID
                     ERVKAAVLYSTVSGDFKDIIERWGPGCLGDVLEGEATFGCNSSDILPLTLPDDLIASY
                     FNGVADPVILKAVSPIYHLKNVTAPVQINYGTEDGKTSAGTPPEWSKKLHEAFQRAGK
                     ESQLIGVEGEEHSFKYDQWFYLMERSAQFFDKYVK"
     gene            2002..4485
                     /locus_tag="PLE39_05500"
     CDS             2002..4485
                     /locus_tag="PLE39_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67813.1"
                     /translation="MKKFLPIIFLLFLLSACASRSSVPATTQPVVAASPNDGEWSGTG
                     NSDEGKSFIVKFTIREEHLAGIVYSFTGTDGISCTGIEYGHLDPADDLLIHDDNLSAV
                     LGEDLSFEAAFVDGRTASGQMTIHWHDRQPRCNGDYEVTWSAEKAATPVEQAAQPRNP
                     NAPDPFETFLQILVFGLSNGAVLALNAIGVTLIYGTVRTLNLAHGDVFALTTALVTSI
                     VNIIGIRENWPLPQLIGALFVVFLLAMALGALLSILVEALGFAPFRGSSRLGPMIATL
                     GLSFILFQGALVWRTYQGSWIPGEHRSVPGLPEVPTDGIPSFLPEINLVRYFGLNSNI
                     IIRFSDVFVILSAVVLVAFANWFIQNSKTGRAIRAVSQNPQAAQILGVNIGGTIRKAF
                     AFGGAFAGAAGFIFALYYSRPFGSHGAQSGLFAFMTALLGGIGNPFGALVSGLLIGGL
                     SSLSDYYLTAQWTPALLLLILILLFAWRPTGFTSEGEGGEAAVARDSIILAGPVQGSG
                     GMRWLFIALLILFAFPLAFQGGQVILRLAGIFVLLALGLNLVLGIAGLLDFGFAASFG
                     VSAYLTALFSSKFDFTIALLLGALMGAFLGWLKGGLARRLRSDFFAVATLAFGLLIRQ
                     VIINLDFTGGMGGIGGIPAPHIFGFNFAGQMQKYYLVFFLIILLAWFSQRVISSRTGR
                     SWMALGDDELAALSVGVDVGRARMRALMLGSAMAGVAGVLYAGGLSFVEPDLMAFHVS
                     SMILTMVILGGAGSVPGALIGAVTIILYDKVFVPQLANWLALIWPVAIGSVPDIRGAS
                     FFNFGIALYLTVLVRSRMASAGSGEARKQ"
     gene            4822..>5127
                     /locus_tag="PLE39_05505"
     CDS             4822..>5127
                     /locus_tag="PLE39_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67814.1"
                     /translation="MSKNQLFEYFDELDDQEGTLAQSNHKRVSVRRESKKRDNEARLF
                     IRAQEFSRDFQFTYKAARFEEAWLLDSLAELADLEWISDVLRKVKGGKEASVYLCKGG
                     "
     assembly_gap    5128..5227
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5228..5611
                     /locus_tag="PLE39_05510"
     CDS             <5228..5611
                     /locus_tag="PLE39_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HNE67815.1"
                     /translation="VDLDRDEAGQLFERVIRNIDLLLRNDRIHGDLSAYNILYWNGDV
                     TLIDFPQVVAPEANPQAWNIFLRDVTRVCDYFKNQGVWSDPRALAAELWTAYGHKTTK
                     EVEPSLLDAEKPEDRKLWMKQSNKL"
     gene            5725..7152
                     /locus_tag="PLE39_05515"
     CDS             5725..7152
                     /locus_tag="PLE39_05515"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNE67816.1"
                     /translation="MNNVRPKLSMLTEEQIQEIHDHTMNVLETVGVRVDSPSALEMLK
                     KKVGASMVNDRLVKIPRDVVEWAIKSAPKQVQVYDRRGKPQFTVGGPEDRIRFGIGVT
                     ALYYQEPDTDTPVLFERKHMRDMVRVGNKLPHYDMVSTVGIVRDVPEHLTDMYGSLEH
                     FVNGVKPLVLLVSDEHKFNDVMEMFETLHGDLGEKPFIIPYFNPVSPLVMNEGTVDKM
                     KIAIERGLPVIVSNYSMSGATTPITPAGTIAVLLAELLAGLVIGQLYKEGAPIFLGML
                     PVYFDMKTLMNFYDPQSILVNLACAEMMAHYGLPHCGTSGSGTGWGMDLLAAETYWMN
                     TLTFSLTKGGIAPFVGDTLGSKAISPQTFIYCHEIIDQALRFSSGFQLDEASVGLSEV
                     EKVGPGKSYVSAPTTLKKYKNGYYVNPIFPRWSMEKWIESGQPHAQQKLREYTIEFLK
                     DLPAPEDYEELHAKGEEFIASRLSK"
     gene            7157..7477
                     /locus_tag="PLE39_05520"
     CDS             7157..7477
                     /locus_tag="PLE39_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67817.1"
                     /translation="MPARVATFVFGIVFAVLFSWLPVYPLLIEGREISESGESLYQEW
                     RFVPLAEYNDSAIFARSAWQKSVRATYVVTTIVNYLVLFGASWGIASLGVRIIKKSAP
                     RLIA"
     gene            complement(7513..8712)
                     /locus_tag="PLE39_05525"
     CDS             complement(7513..8712)
                     /locus_tag="PLE39_05525"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNE67818.1"
                     /translation="MNNRSLLATRNSEFFLYEELTWDAVAELPRDTPLILPLGSGYNL
                     DLLADQLSNPPRIGLLPPFPFGWRGSGLELPDAIFTQYIVNLMDSLRDDGFSRVYCLA
                     PQGLDPQSSFILQHSASILRQPHPSLYEAQTFLPPDSERGKVILIPIGHTEQHGYHLP
                     LSVDTIIINSIAQGTAAKVPTRSWAMPVMPYGVSTHRSSFAATLNAGGRAFEDFWGAA
                     IDVLVARGFDRFYLMSGHGGNTSFLVNIVKYAGERHRRIFCATAFLHTSGRIGAEALD
                     KYRTSKIGGMGHAGELETSYLLHLRPDLCQMDRVVDETDFVSTPDYYMDWIEGGSLVA
                     NPPWDDDTKTGAYGAGSHATAEKGRLWLEAAIEEKADHVEQIHEQHERREKRRNEGYG
                     LWGKFRK"
     gene            complement(8732..10585)
                     /locus_tag="PLE39_05530"
     CDS             complement(8732..10585)
                     /locus_tag="PLE39_05530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNE67819.1"
                     /translation="MDNSVKLTEKYERNGWPSVKRPDLNPPAGLNLSLLTSLERIRSH
                     ALSSQGQISCIKDGESLSDVYTMQANGSWLQRISTERPLVPYWDDEIPEWSPDGNWLA
                     FGIKGHVHIVPRDGGLPKKITDFAVAASGPRWMPDSIGLIVTVERNEADQLLLTNYNG
                     DWPRALTTDTIGDHWDARPSPDGKFIVFNLRRFDDLNRLDIVLLEIATGKQTTLYGKP
                     STRAVSPKWSPDGKWISFISQETGREELYLIKPDGEGLHQLTKAGKDIFQYEWSPSGK
                     QMLVVENNGGSFELMLLETESGSRSALRSEKGIHSNPNWAKDESFITFEFEGPTLPPD
                     VYRMELPSKKITQLTFSNPPALQRNKFIVPEVVSYKSYDGLEIPAFLYRPEKSNGAAI
                     LYPHGGPKDQYGFGWDELAQYYVAKGYTYLAPNYRGSTGYGQEFERANYNDWGIGDTQ
                     DCLHGAKYLRTLKTIKPDRIAITGGSYGGYMTICSLSRDPEYLFACGITKYGDSNLVS
                     SWAQCEKRLRVYTEIFLGHPSENLQIYLKGSPITEAKNVQKPVLILHGLLDSIVPPEA
                     SEEWVEALRREGKTFEYKTYGDEPHGFLRRENLLDVYERMERFLDWYLLPS"
     gene            10779..12407
                     /locus_tag="PLE39_05535"
     CDS             10779..12407
                     /locus_tag="PLE39_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE67820.1"
                     /translation="MKRRFLFILVILTLVLSACGGGNEKAIVRIGWAGSPDNLNPGMG
                     ILTESYTIYNLVYDTMYSLNLDGSFTLSIADSVERSDDGLVYTYKIKDGIKWHDGKPL
                     TAEDVAFSYNLYKDTPEYPYLNGYYTTYFASVESKGNEVILTLTEAIPNIESQLVFLY
                     ILPKHVWEGVNKLEYENFEMIGSGPFKMAEYAQNEFVRLTANKEHFSRPPKVDEVVFQ
                     TFENQDALVQGLKTGQLDMITEMPNTSVETLKAEENVEVVIGAPFSPGVTDIIFNQID
                     PDNCPTDAGGLCTGHPALRDRNVRLAMAHATDKQKLIDLILLGLGTPGLTLIPDGLGV
                     WYNNSIKDYEYDVAAANKILDDAGYKDTDGDKIREMPDGSKPLTFRLNWPSDSINAPR
                     MAELLSEMWGEIGISLEMQAVDPDALTAQCCPAFDFDIMIWGWGSDPDPSALLYVYTS
                     EGIPTGSSETGYSNPKYDELYAQQQTELNFEARKSIVWEMQKLVHDDVVYIIPFYDAE
                     TQAYRSDRFTGWITNQPKVELSDVTSLVLIEPVK"
     gene            12469..13497
                     /locus_tag="PLE39_05540"
     CDS             12469..13497
                     /locus_tag="PLE39_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE67821.1"
                     /translation="MNRYQILLRKTAWALFTILFVIILNFFLFRILPGDPARAGIRDP
                     RLKKESIEILRVRFGLDKPIINCFESLNPIKVGSCEVNPLETQFFIYVRNLLQGEFGF
                     SYHTNRPVGEILAERLWNTVLLIGAGQILSILIGVAFGIIAAWKARTTVDHAAITFSL
                     VAWSLPTFWLGIILLFWGSQNGLPLAGKSTPGMSSMPLLQQWGDLFVHMFLPTLTYTL
                     IYMAEYTLIMRSSLMDVLSEDYILTAKAKGLSNFQILKDHALKNAMLPLVTVIAINLG
                     FTVAGAVQIETVFSWPGLGSAIFEAVNRRDFPVLQGAFLLIAVAVILANFLADLTYTY
                     LDPRVTME"
     gene            13502..15421
                     /locus_tag="PLE39_05545"
     CDS             13502..15421
                     /locus_tag="PLE39_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HNE67822.1"
                     /translation="METTVQVVETKSRFHGFWRSLRKNRIGFIGLVMLAVIVLLALFA
                     PFIATHDSSSSASAEIGDIYNPPSAEHWLGTDDAGQDVFSNFIFGARISLIVGFFAAF
                     ISLIIGGMLGLVAGFFGGRVDNVIMRFTDIMLVIPDLPLIVVIVALTKPSLLNIIFVI
                     GLLGWTTTARVVRSQTLAVKSRKFVLRARAIGAGRRHIITNHILPLVMPILVVQAVLV
                     VSSAILSESALSFLGLGDPSALSWGQMLNYAFGRGAMSIGAWWALFVPGFGIVWVVLG
                     LTLLGQGLEQVLNPRLDTHHLTPGRPTVQHEAAQPSEQKSEKLLEVRNLSVNYVNEGK
                     PPARAVENVSFTLKPGELIGLVGESGCGKTTLMLALLRLLPSAGQVVNGSVNFHGRNL
                     TELGDDELNQVRWKGISIVFQGAMNALNPVRTVGEQIAEAIIKHLPDFPRDARLQERV
                     VELLDLVGIAADHKDHFPHQYSGGMRQRAMIAMALACNPQVIIADEPTTALDVMIQAQ
                     ILELLDSLRKKLGLAIIFVTHDLGVVAEICDKVLVMYGGVTAEYADVDTIYNEPRHPY
                     TQELIKAFPDLTKPKKKLSSIPGYPPRLDDLPAGCRFAPRCPQAFDRCRTEQPVLYTV
                     KEDHVTSCHLVEASK"
     gene            15418..16410
                     /locus_tag="PLE39_05550"
     CDS             15418..16410
                     /locus_tag="PLE39_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE67823.1"
                     /translation="MSDDQFLKVRGLKKYFDAGRPGLLFGSRTPRYVHAVDGVDFTLR
                     RSEVLALVGESGCGKSTLSLLLMGLEDPTEGNIIFEGRDITHLQDSERKELRRKIQMV
                     FQDPYESLNPTQTIEEIVTEPLIVHNLETEKLERDSRVKKALEDAGLKPAEVYLNRFP
                     HELSGGQRQRVVIAAALVLEPEILLADEPVSMLDVSIRAEIINLLAELRITRQIAVIF
                     ITHDLGSVGFFADRVAVMYLGRIVEIGTMLEVLEKPQHPYTKALLSVIPVPNPRMRRE
                     RIILQGETPNPIDLPTGCRFHPRCPSAMPTCKVSDPPFEVFTKTHQAACLLLVK"
     gene            16434..17213
                     /locus_tag="PLE39_05555"
     CDS             16434..17213
                     /locus_tag="PLE39_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNE67824.1"
                     /translation="MDITSLQNPKIKYIVKLREDKRLRRKDGLMLVEGRDELTLALST
                     GLKPHTLLTAPELATVPMDISSAEITTVTRAVFEKISYRDNPDGWLGLFPIPQTDLDD
                     LKLGANPFVIVAESVEKPGNLGAILRTADAAGVDAVIVCDGRVDVWNPNTVRASRGTV
                     FTVPTVEADSQMTLNWLRLRKMRVLAATPSAEVLHTSIDLSQPIALAVGTEDEGLTDF
                     WMQNADIKVKIPMLGKVNSLNVSIATALITYEAVRQRNGKS"
     gene            17225..18172
                     /locus_tag="PLE39_05560"
     CDS             17225..18172
                     /locus_tag="PLE39_05560"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNE67825.1"
                     /translation="MKALWLEDNQISLRDVAKPKNPNEALIKIRKAGICSTDLELVKG
                     YYPYTGILGHEFVGEVVEAPDAAWIGQRVVGEINAVCGTCETCLSGRPTHCENRTVLG
                     IVNRDGVLAEYATLPLNNLHRVPDSVSDDMAVFTEPLAAALEIQQQIQLRPDDRVLVV
                     GAGRLGQLIAQTLALTGCDLQVVTRHAHPQSLLAARGIRFTTVDEVQPRKWDVVVEVT
                     GSPDGFNLARKAIRPRGTLVMKSTYKGDMNVNFSSIVVDEIQIVGSRCGPFEPALRLM
                     EKREVDPTVLIMEQYKLNDVVRGFEHAARPGVLKVLIEM"
     gene            complement(18282..18593)
                     /locus_tag="PLE39_05565"
     CDS             complement(18282..18593)
                     /locus_tag="PLE39_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67826.1"
                     /translation="MSTLREKRTYFGLAALIIGGISVLDLAARFAVAYMDIAPRTFGI
                     LNQITTLFFCILTPLAFVLAVLGWRRKNDSRSQAGIALALVLLPFAGLLLQFILNFFI
                     Q"
     gene            complement(18602..19456)
                     /locus_tag="PLE39_05570"
     CDS             complement(18602..19456)
                     /locus_tag="PLE39_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNE67827.1"
                     /translation="MLETPSAGRHRQIFWSVLGLLLFLRIPYTLAIIYFLPIENQNGA
                     AVYEVGTYFLTAFLIWWERENLANLHIDTTALLLIIFLRPIQTLILAYWKVETPLVFP
                     GWPSLLIWIIAIGLGLALWQSGFKSTRISFLTLAWLSFGLLIGAVTSIAENFSSFQSM
                     VTHPRAFSSAVLLSTSLNLFYHLGFAPINEEPLFRGFLWAALRQLKWKDGWILVFQAV
                     LFTSAHVYFAGQYPFMFWVFIPAAALLFGWLTLRSRSIAPAMLAHGMINGSVYLWLVS
                     ILSAGSGL"
     gene            complement(19460..20458)
                     /locus_tag="PLE39_05575"
     CDS             complement(19460..20458)
                     /locus_tag="PLE39_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE67828.1"
                     /translation="MKTEIKPFSAEMIPLAGNLLSARHKRNRAKLSLLPERFEDSTVA
                     GKAVEALWQKKFKNGYAAFRDGKMTAYLIGDFTVQPWGRCGYVYLAGYALEEGESLKT
                     LQDLYSLLGEDWVQKGVFSHSLYISAADADVIDALFALGFGKERVDALLDLGIAEIAE
                     PAAPANIDIRIAGTGDNSHLGDLSHVIMESLANAPYWHPTIPEDWEELREGWAELADD
                     QEWTVWLALEKEKALATVGFRPEEEQETAMLVSPRTVYLSIAATLPQARGRGISTALT
                     LCGLKQVQKEGFNICYTNWISPNLPASRFWPRFGFKDAAYRLAKRIDPMIAWTKQR"
     gene            complement(20536..22437)
                     /locus_tag="PLE39_05580"
     CDS             complement(20536..22437)
                     /locus_tag="PLE39_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE67829.1"
                     /translation="MTDELIELEEEEYTSNLTMPVLRRIGGLLKPHWRWVLGFLVTIA
                     LVSSMDAYFTYLNKQIVDQGIKFSDYERVSEIAWMYGSFLILQAAFIFIFIYLAGVLG
                     ERVQYDLRKMLFNHLQDLSLSYYAQNAVGRLIARVTSDTGRVSDLVTWGIVDSVWAVM
                     NITTSLTFMAIINWRLALIVSVIIPIMMFVATKFQKYILVEFRQSRRANSKITGAYNE
                     NIQGVRVVKALGREDENLVEFQQLTTRMYRASYRAAWLSALFLPTVQIIAALALGAIV
                     WYGGVQIQVGLITIGGIQAFVSYLTFMMWPVQDLARVYAEMQHSIASAERIFKLVDTP
                     PEVHNRSDAIEAKTLLGEIEFDHVDFYYEDRKSVLTDFTLKVKPGEMIALVGPTGGGK
                     STIVNLLCRFYEPRNGVIRINGHDYMDYTLESIHNKIGIVLQTPHLFSGTVRENIRYG
                     NLSATDAEVEEAAKIAGAHDFIVTLEKGYEQNVGESGNLLSVGQKQLISLARAVLARP
                     ELFIMDEATSSVDTLTESLIQRGMEALMKGRTSFVIAHRLSTIRRANRILVIENGSIA
                     EQGTHAELLRQRGHYYRLYTQQFRHELEVAYGVTEEIDGNERANVTADSIMVRRERET
                     AGEEAIAAD"
     gene            complement(22487..24325)
                     /locus_tag="PLE39_05585"
     CDS             complement(22487..24325)
                     /locus_tag="PLE39_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE67830.1"
                     /translation="MSTTTTSPYFDFRDALHTNRLVGLWRMMTDFRLSYVAATLALAV
                     SALAKTSVYLLLRFFADDVLKKGTMIGTTMTQTFLYIGLGFFILALFEGGFAFLSGRL
                     AAYTAEGITRRLRDFLFDHIQRLSFSYHSATPTGDLIERVTSDVDALRRFFSEQAIGV
                     GRIILLFVINWVAILYINVKLGLLSVVVIPPILWVSLWFFKKVTKAYEDYQAQEALLS
                     TTLQENLTGVRVVKAFARQDYEKEKFEKDNWGKYLKGKLLLLMHSLFWPLSDIVLGAQ
                     MLFGFVYAAFMAINGEITVGDYVAYVGLVVWLIFPIRNLGRTIVQTSTGMVSYARLMD
                     ITKQAREPLTDGSIQPAGPARGEIEFKNVGFIYSDGTHDVIKDISFHIKPGQSIALLG
                     STGSGKTSLVNLLPRFHDYTSGQILLDGVELKEYPRKYLREQIGIVQQEPFLFSRSIR
                     ENILYGVGRSVTQEEVEAAAKAAAVHDVILTFPDGYNTIVGEKGVTLSGGQKQRVTIA
                     RTILKNPRILILDDSTSSVDTETEASIRNALNGLMENRTTFIIAHRIQSVMKADLILV
                     LDKGQVIQKGNHEELLKQFGGMYRKVYDIQTRIDEELEQEIMKSEL"
     gene            complement(24410..24595)
                     /locus_tag="PLE39_05590"
     CDS             complement(24410..24595)
                     /locus_tag="PLE39_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67831.1"
                     /translation="MKPANNFHKFLDTSQIKGVNSASQYEKSKERSALLMFNTIFYTF
                     PEAVGILPSRSAPADRR"
     gene            complement(24620..25981)
                     /locus_tag="PLE39_05595"
     CDS             complement(24620..25981)
                     /locus_tag="PLE39_05595"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE67832.1"
                     /translation="MNQLFVSYSRKDADFARKLTNSLESEKMSVWIDWQDIPPSVDWF
                     KEIEKGIEEADVFIFVVSPDSILSPVCTDEVAHAIKNGKRIAPVVAREIDMKDAPKSL
                     SHLNWVFFSRPPDTFEEAFNKLLTAIRTDYDWVQTHNRLQVKALEWDRNKREDSFLLH
                     GMELLDAEAQLKVNGEKQPLPTSLQRTYISASRVAENEFIEAERAEAEQLKLEQEMRL
                     RLRRVTFLVLAVFTLAFFALYAWLSRVTTDLALNSIKNQMLALVETSVSIVDGNEYQS
                     FASAYADPSDTAVYSDEYYQRLTRYVDGIRAGNTNIPGEIVLYTIVKGGNSGEFSVLV
                     STMEDLPFRFSITSNNPSDIHAVGMNETVADPQTIYQNENGGWVCSCAPIYNDNGESV
                     GALCADFDANLVEETKRRVKTILTLGFIAIYPVMIGLTLMATRNVQKRNAQIPAETKP
                     SAS"
     gene            complement(25986..27365)
                     /locus_tag="PLE39_05600"
     CDS             complement(25986..27365)
                     /locus_tag="PLE39_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNE67833.1"
                     /translation="MFNKIVALYSDREYFNNVFKIALPIIFQQFVYALLNMLGVVFVG
                     QKGDTAVAAVGLAGQIAFLLNLVHFGIISGAAMFTAQFWGRQDIPNLKRVLGLCLMFA
                     ISASAVFFAISQFVPEWFLGIYSKDPEVIAMGTEYVRTFSWTFLFMAVSASYVFVMRS
                     TGSVKLPTYVSVGVLSLNTLLSYVLIFGYFGMPEMGVQGAAVAAVIARALECVILLYV
                     IYAYKFPVAASIKELTDFDLAFTARVIKPMLPVMLNELFWSLGITTYNAIYGRMGTEA
                     LATVNIVSPIEQIAFVVFVGLANATSVLVGNRIGAGKDDEAYLYGGRSIGLGIAGGVL
                     LGLLLQIFKVPALSLFNVSSEVLQNASVMVNIVSIFLWVRVNNMTIVVGILRAGGDTR
                     FSLFLDGIIIWLVGVPFAALGAFYFHLPVYFVYLCVMSEEVTKWILGIFRWRSRKWIH
                     NLASQMEGI"
     gene            27440..28084
                     /locus_tag="PLE39_05605"
     CDS             27440..28084
                     /locus_tag="PLE39_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67834.1"
                     /translation="MKRLLILLIFVITACTPAFVDITPTPASPLPDASPTETTSPQVD
                     SATNTPVELESNVDILANLPNPDCAALTPADQEGPYYTPDTPERNSLIDEGMQGTKLI
                     LAGFVLTADCLPVPNAWLDFWQANANGEYDNEGYTLRGHQFTDNKGRYYLETILPGEY
                     PQRPIEHIHVKVQLPAGEVFTTQLYFPTQPVNGLTVQLEDKGDHFIAYFNFVMK"
     gene            complement(28146..29840)
                     /locus_tag="PLE39_05610"
     CDS             complement(28146..29840)
                     /locus_tag="PLE39_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE67835.1"
                     /translation="MTDQMIGQQLANFRVERLIGQGGMATVYLGQDVKLQRPVAIKVI
                     DKRYKNDPAYTSRFIKEARMMAKWRHQNLIQIYYADEEKGYSYYVMEYVDGNDLSTVM
                     EMYAEEGELMPIADVLRIGNAVAGALDYAHSQGVIHRDVKPSNVLIANDGRVLVGDFG
                     MAMEVSDGSMGNIFGTPHYISPEQARRSADAVPQSDLYSLGVILYEILTGAVPFNDPS
                     PASIALQHISQPPPSLRSINPEISPAVEAVVLKALDKNPKNRYQTGAQLMSALDEAFQ
                     AANSSPKIPLPPLPVGVPTVRRSGIPMDRITKRNSGKVKGNTIHQPVELPATVRAANQ
                     NRKKINGWLVVLALLLFLGIGGWYLFKNRPASQTDSPTAAFSPAPLNSTVEPAPPTET
                     LISASATPVPPPTVEASPIPTLAQEAAPTAIPTVKYRDGNNLTLFWNETSFHMLNRSG
                     QPRSLSGFSFERLDENGKPNDRFAGHLWENLKFNYIPSKYCVSIKIYKDEDPPYIDPP
                     DCHGGYVSIIQPRMDLDAELFFWTPKEGSSQFRVVWVGEEVARCDISAGTCEVYIP"
     gene            complement(29837..32224)
                     /locus_tag="PLE39_05615"
     CDS             complement(29837..32224)
                     /locus_tag="PLE39_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67836.1"
                     /translation="MTATNLRTVAKTISVQQLSRLSLPEVEAVVDLVSKVLPAGNVPG
                     MILSGLARLPGRRIPVQKMHQDINALFSGVEQILDSAMYGAIFAGPAAIIWGYQHLLK
                     LAGKDPETAFPEGVWQFYASYALREDTARHTNETHGFDSLLRAHKIQLSPVDRLTAWL
                     MASVTCLHQYDRLLENEWRERVSISTLEESSSERAKRLHRDWQLHLPYRREEDAPDLD
                     YPAYRRFKFDQFLKANLTSLPKQTYEKWEARLNLLTQNSLPAYQRQMSILAYLEPGSY
                     GESRIPFDLTDAQIGIIHRDSYYLFPVCEQGSNKPLNVMTARAQIANLLRSPFSSPSQ
                     IASLTRIKRSALTELRGKLNPMLVNELDNLKFAPILISTDMRSPALPLSELRQTERGI
                     GSHALTIFDTGETMVFDQSHIFFDGAWGTALSEIMTNEALSWARYLSMLPSPTPADTR
                     VYTALTLQLQPADVDLVQQAPHVSPESGAETDRVKLKECLLLRKKFKQRNDQIQLTIN
                     DLLVLYRAIHAATYKPSAHLLEEITKLGATKPDVAAAIRQVVEEGSRTNPAILIPMDA
                     SLKVPRERIYPLNMEVPLHELNLLSLHAQTMKLLAAYEDSKEDRAAIYSSFDQMQRMY
                     LASLAGFGLYLNRAKEIAIQGESASVGAIKLLAHLPSPIQRILNKIPERFELLNNLLK
                     GREVFSNVGAVVPMSTLTRFSTAKDDNEQKQLAWGVLTDAKSVMHISLRDFRPQVATL
                     FSIGRKDLANLITQDYLDAYADGFNKFIGDLSRITQASRETLPTSKTRKRNPENR"
     gene            32334..33161
                     /locus_tag="PLE39_05620"
     CDS             32334..33161
                     /locus_tag="PLE39_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNE67837.1"
                     /translation="MTISVFQLTKRGPHTIATHARTLDEITRELPQGFYTTFSTLNAG
                     TEVLGLHMHLQRLYVPAKELSFSPAVDELTLRREIAELARSFLPKEARIRLILAKNDA
                     SMYIVLQQFDALPKKVYEEGVQVVTASLARMAPRVKDTGFISSSNHQRRLVGGDIFEV
                     LLTKEGKILEGMTSNFYAVKKGTLITSQRGILLGVTRRAVLRLARGEGMSVEYRPPQV
                     DENFNEAFLTSSSRGVVPIVSIDGKPVGQGSVGEWVKRLMKAYAEYVERKAERIDGW"
     gene            complement(33187..34221)
                     /locus_tag="PLE39_05625"
     CDS             complement(33187..34221)
                     /locus_tag="PLE39_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNE67838.1"
                     /translation="MSTDLEQKNVSISEAIQQYLRMVERARSRKTMLAYKNALNVFCQ
                     MLNEKSTNPNTTPITQLNEEQFSKFIDYLSPYTTATEQLYLQAVKGFYLFLDSEKLTE
                     VNQSRLTVFMRQRARRPGKHAPPYKIDEIDKILKQARNVQNLVNLGAEEGEKNAKLRA
                     YRDRAFLFMLAETGLRVHEACNLRRGDIDWNEGRAIITGKGNKQAVIRFTTYALQVVK
                     EYLAQRAELDGASGKQLSSLPLFARHDKGAGKKIKPITTTTGRNIVNECVERFLGQDL
                     VGEITPHTFRHYFVTTTLRATGNLAIAQRLARHENIQVTNRYTQLADEELDKAYYDIF
                     EKDSKWKHDD"
     gene            34787..35452
                     /locus_tag="PLE39_05630"
     CDS             34787..35452
                     /locus_tag="PLE39_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE67839.1"
                     /translation="MPPAGLKLLYPVKPTFPITQRFGENPQWYPITNGHNGIDWGIPL
                     RTPIYATLPGSVVRVAADNSGYGNHIRISHDNNLLSLYGHMDERNYALVKVGDTVTAG
                     QHIGYSGNTGRSTGPHLHFEVREGSTAFNPEPYLSDNINIPPARTLFKARVLETTQPF
                     LNIRSGPGILYPVIARYSPGDEMEVTDIGGDTVWLQTKKGFVAYRIDKKTLIEILGME
                     DEG"
     gene            complement(35544..>36607)
                     /locus_tag="PLE39_05635"
     CDS             complement(35544..>36607)
                     /locus_tag="PLE39_05635"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE67840.1"
                     /translation="FNCHAALTRLAAAEPAPVPSQITFEKDTAHPVPVVQKDVLIVGG
                     GPAGLSAAVELKKQGIQNILVVDREPEAGGMPRFCQHTGFGREDLWRMWSGPKYAKYY
                     RDLAEKMDVEVQTSTTILGWAGPAKLSFTSPSGVGEVDAKAVLLATGVRERPRAARLI
                     PGTRPQGVFTTGSLQRFVYQERLPVGKRAVIVGAELVSLSALMTLMHAGVKCERMVTE
                     EARHQIEFPYIVMKWALADILTRTPITPNARISNIFGRRRVEAVELTHASGQTETVEC
                     DTVIFTGSWIPEHELARTGGLILNPQTKGPQIDADFRSSVHGVFAAGNLLRGVETADH
                     CAIEGQQVARSIARFLKGK"
BASE COUNT         8413 a         9275 c         9791 g         9028 t
ORIGIN      
        1 ggcgggcaga ttcagatcct ccaaactttg atgcaggtgg aaacctacac acgctacctc
       61 atctcctatc cttccgatgg actgacgatc acaggcattt tgcaagtccc agcaggggag
      121 gggccgtttc ccgtcatcgt catgaatcac ggctactaca accgcggcga atacaattca
      181 ggcgatggct cggaccgtgc tgccgcctac ctcgtcgagc gcggatatat caccgtctct
      241 tccgattacc gtacctgggg cgggtctgat atcggctatt ctttttttca cacaggcctg
      301 gtggccgatg tgatgaattt gatcgcttcg ctgccatcca ttccgcaagc cgatgcgacc
      361 cgcgttggca tgtggggaca tagcatgggc ggcggcatca ccacaaaaat actcacactt
      421 gagtcgcctg tccgcgcggc ggtgttgtac gcgcccaact ccgccgacga tgccgacctg
      481 atcgctcgct ggggttatgg ctgtatcggc gatatcgctg ccggggaatt gctcaatacc
      541 tgcaatgccg ccgatgtcat tcccgtcacg ctgtcagatg agcttattgc tgcatatgtt
      601 gaatccgcta ccagcgcgga gaggatgcgt gagatcgctc cgtattatca ccttgaaaat
      661 atttccgtcc ctgtgcagat tcatatcggc tcggcagatg gcgatttcat cggcgccact
      721 ccgcctgaat ggtcgtataa actaaatcaa ggcctgctcg atgcaggcaa agatgtggaa
      781 ctgtttgtat atgagggcca gcgacattcc tttaccgggg aggcttggtt cactttcatg
      841 cagcgcgccc tcgatctgtt cgatgagcaa ttaaaataag ccgatgaaaa caaagtcaat
      901 gcgtttttgg gtttgcttgg tcttgtttgc gctttcagcc tgccttgccg caccgttgag
      961 cccaccccct gtacccgcgc ctgttgaaac tgccacaaag atccccgtca catttacgcc
     1021 ttccgtaacc ccatcaccga ccgccacgcg cacaacacag caggctttgt atccattcac
     1081 cattgaggga ttgcgaaagc ataaattcca aagcggcgag attatcatcc gcgagaccct
     1141 gcttgagacc gaagtgttca ccagctatct cattgaatat cccagcgacg gcctgaccat
     1201 cactggagtt ttgcaaattc caaagacggg agaaccgccg ttcccggtca tcgtgatgaa
     1261 tcacggtttc ttttcgcgct acatttaccg ctctggcgat ggcacagacc gtgccgccga
     1321 gttcttgaat cggcgcggat acctcaccgt ctcttccgat taccgcagtt gggccagcgc
     1381 agacacgggc gaaagtttgt tttactcggg ccttgccatt gatgtcgtca atttgatgaa
     1441 cgcacttccg tccattcccg aagccgatgt gacccgcatt ggtatgtggg ggcacagcat
     1501 gggcggcggc accaccttga aagtgctcac cattgatgaa cgcgtcaagg ccgcagtttt
     1561 atattccacg gtcagcggcg attttaaaga tatcatcgaa cgttgggggc caggctgtct
     1621 tggggatgtc ctcgaaggag aagccacctt cggatgcaat tcttccgaca tccttcccct
     1681 gaccctgccc gatgacctga tcgcatccta ctttaacggg gtggctgatc ctgttattct
     1741 gaaagcggtc tctcccattt atcatttgaa gaacgtcacc gcacccgtgc agatcaatta
     1801 cggcaccgaa gatggcaaga cctcggcagg cacgccgccg gagtggtcga aaaaactgca
     1861 tgaagccttt cagagggcag gcaaagaatc tcaactgatc ggtgttgaag gtgaggaaca
     1921 ttcattcaag tacgaccagt ggttctattt aatggaacgt tcggcgcagt tttttgataa
     1981 atatgtgaag tgaaattgcc aatgaaaaaa ttcctcccga tcatttttct gcttttcctg
     2041 ctttcggctt gtgcaagccg ttcttctgtg cccgcgacca cacagccagt tgtggccgct
     2101 tcacccaatg atggcgagtg gagcggaact gggaattccg acgaagggaa gtcttttatc
     2161 gtcaaattca cgattcgcga agagcatctg gctggcatcg tgtacagttt taccggcacc
     2221 gacggaatca gctgcaccgg catcgaatac ggccatttgg accccgccga tgacctgctc
     2281 atacatgacg ataacctttc cgcggtcctc ggggaggacc tttcttttga agcggctttt
     2341 gtggatggga gaactgcctc agggcagatg accattcact ggcacgaccg tcaaccgcgc
     2401 tgcaatggcg attacgaagt gacatggtcc gctgaaaaag ctgcgacccc ggtggaacag
     2461 gccgcccagc cgcgcaaccc gaatgccccc gatcccttcg aaaccttttt gcaaattctg
     2521 gtcttcgggc tttccaacgg agccgtactg gctttgaatg ccatcggcgt taccctcatc
     2581 tacggcacgg tccgcacttt gaatttggct cacggcgatg tcttcgctct gacgaccgcc
     2641 ctcgtcacat ccattgtgaa catcatcggc atccgtgaga attggcctct gcctcaattg
     2701 atcggcgcac ttttcgtcgt gtttcttttg gcaatggcct tgggggcttt attgagcatt
     2761 ctggtggagg cgttggggtt tgctccgttt cgcggttctt cgcgtctggg gccgatgatt
     2821 gccacgctcg ggttgtcttt cattctgttt caaggggcac tggtctggcg cacctatcaa
     2881 ggttcatgga taccgggcga gcatcgcagt gtgcccggcc tgcccgaagt gcccaccgat
     2941 ggcattccct cgtttttgcc tgaaatcaat ctggtgcgat actttgggtt gaattcgaac
     3001 atcattattc ggttcagcga tgtgttcgtg attctttctg cagtggtgtt ggtggcattc
     3061 gcaaattggt ttattcaaaa ttcgaaaacc gggcgggcga ttcgggcagt ttcgcaaaat
     3121 ccacaggcgg cgcagatcct tggggtgaat atcggcggga cgatccgcaa ggcgtttgct
     3181 tttggcggcg catttgcagg ggccgcgggg tttatctttg ccttgtatta ttcgcgtcca
     3241 tttgggtcgc acggtgcgca aagtggattg tttgcattta tgaccgcgtt gctgggcggt
     3301 attgggaatc cgtttggggc attggtcagc gggttgttga tcggcggcct gtcttcgttg
     3361 agcgattatt acctcaccgc gcaatggaca cctgccttgt tgctgttgat tctgatcctg
     3421 ttgtttgcgt ggcggccaac aggattcact tcagaagggg aggggggcga agcagccgtc
     3481 gcacgtgatt cgatcatttt ggctggaccc gttcaagggt caggtgggat gcgctggctc
     3541 ttcatcgccc tgctgatttt gtttgcattc ccgctggcgt ttcagggtgg gcaggtcatt
     3601 ctgcgtctgg ctgggatttt tgtcctgctg gcgcttgggc tgaatcttgt gttgggaatt
     3661 gcgggactgc tggactttgg ctttgccgcc agtttcgggg tatcggccta cctgaccgct
     3721 ttattctctt cgaaatttga ttttacgatc gcattgcttt tgggggcgct gatgggtgca
     3781 tttctgggct ggctgaaagg cggcctggca cgccgtttgc gcagcgattt ttttgcagtt
     3841 gccacgcttg cctttggtct gttgattcgg caggtcatta tcaaccttga ttttacaggc
     3901 ggcatgggcg gcattggggg aatccctgcg ccgcatatct ttggtttcaa ttttgccggg
     3961 cagatgcaga aatactacct tgttttcttt ttgatcattt tgcttgcatg gttcagccag
     4021 cgagtgattt cttcgcgcac tggccgctcg tggatggcgc ttggcgatga tgagctggcc
     4081 gccctgtcgg tgggtgtgga tgtgggacgg gcgcgcatgc gggctttgat gcttggttcg
     4141 gcgatggctg gagttgccgg ggtgttatat gctggcggct tatcgttcgt tgaacctgac
     4201 ctgatggcct ttcatgtctc aagcatgatc ctgacgatgg tgattttggg aggcgcaggc
     4261 agtgtgcccg gagcgttgat cggcgcggtg acgatcattt tatacgacaa ggtatttgtt
     4321 ccgcagctgg caaactggct ggctttgatt tggcctgttg cgattgggtc ggttcctgat
     4381 attcgcggcg cgagtttttt caattttggc attgcgttgt atcttaccgt tctggtgcgt
     4441 tctcgaatgg cttcggctgg atcaggtgag gcacgcaagc agtgatgcct gaataaaaag
     4501 caagccgtgt tttcgcggcc atattgcgtt cgcacattct tgacattggt ttcccgacag
     4561 gataaaatca cgtcactata tttcactttg aaaggaggca cgaaaaaatg acatccatct
     4621 tgttatctgt caacattctt gtttgcgcgc ctcctgttgg tactgtgtag tacggcttta
     4681 tttctcgtac attgaaccgc aggtgcgcca tgccctgcgg tttttgattc catgctgcag
     4741 ccgcgggcca aaaccctgcg gttttgcttt taacgcgggg aaacgtcgga tgacttttac
     4801 aaaaataagg taattaaaaa aatgagcaag aaccagcttt ttgaatattt tgatgaactc
     4861 gatgaccagg aaggcaccct ggcgcaatcc aaccataaac gtgtgtcggt caggcgtgaa
     4921 tcgaagaagc gcgataatga ggcgaggttg ttcatccgcg cccaggagtt ttcgcgtgat
     4981 tttcaattca cctacaaggc cgctcgtttc gaggaagcct ggttgttgga ttcgctggcg
     5041 gagcttgccg accttgagtg gatctcggat gtgcttcgaa aggtgaaagg cggcaaggag
     5101 gcttcggttt acttgtgtaa aggcggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnngtg gatctggacc gtgatgaggc aggccaactt tttgagcgtg tgatccgcaa
     5281 cattgacctg ttgcttcgga atgaccgcat ccatggagat ctttcggcgt acaacatcct
     5341 gtattggaat ggcgatgtta cgttgatcga ttttccacaa gtggttgccc cggaggccaa
     5401 tcctcaggca tggaacattt tcctgcgtga cgtcaccagg gtttgcgatt attttaaaaa
     5461 tcagggagtt tggtcagatc cgcgcgcgct ggctgcagag ttgtggacag catatggtca
     5521 taagaccaca aaagaagttg agccatcctt gctggatgcg gaaaaacctg aagaccgaaa
     5581 gttgtggatg aagcagagca acaagctcta acctgaatct gcgcagggaa ttgggcaatc
     5641 gccatcaaaa ggcagccgtt gacgagagtt gcggctgcct ttttcatgcc agtgataaaa
     5701 ttgcggcgag gagatcataa actcatgaat aatgtccgcc caaaattgag catgttgact
     5761 gaagaacaga ttcaggaaat ccacgatcac accatgaacg ttcttgaaac tgtaggggtg
     5821 cgggtggatt ctccttccgc tttggaaatg ttgaaaaaaa aggtcggcgc atccatggtg
     5881 aatgaccgat tggtgaaaat tccgcgtgat gtggttgagt gggcgatcaa atctgctccg
     5941 aagcaggtgc aggtgtacga tcggcgcgga aagccgcagt ttacggtcgg cggccctgag
     6001 gatcgtatcc gctttgggat cggtgtgacc gccttgtatt atcaagagcc agatacagac
     6061 accccggtat tgttcgaacg caagcacatg cgtgatatgg tgcgtgtggg gaacaaactt
     6121 ccgcactatg atatggtgtc cacagtggga atcgtccgcg atgttcccga gcatctgaca
     6181 gatatgtatg gcagcctcga acattttgtg aacggagtca aaccattggt tttgttggtc
     6241 tctgatgaac ataaattcaa cgatgtgatg gaaatgtttg aaacgcttca cggcgatctg
     6301 ggagagaagc cgttcatcat cccgtacttt aatcccgttt caccattggt gatgaatgaa
     6361 ggcaccgtgg acaaaatgaa gatcgccatc gaacgcggat tacccgtcat cgtttcgaac
     6421 tacagcatgt ctggtgcgac gaccccgatc acacccgcag ggacgatcgc ggtcttgctg
     6481 gctgaattgc ttgcggggtt ggtcataggt cagctttata aagaaggcgc acccattttc
     6541 ctcgggatgc tgcccgtata ttttgatatg aaaaccctga tgaatttcta cgatccgcag
     6601 agcattttgg tcaatcttgc ctgcgctgaa atgatggcgc attatgggct tccgcattgc
     6661 ggcacatcgg gcagcggtac tggctggggc atggaccttc tcgcagccga gacttattgg
     6721 atgaatacgc tgaccttttc tttgacaaag ggcggcatcg ctccatttgt cggtgatacg
     6781 cttggatcga aagcgatctc cccccagaca tttatttact gtcacgagat cattgaccag
     6841 gccctgcgct tctcttctgg ctttcaactt gatgaagcat cagtgggctt gagtgaagtc
     6901 gaaaaagtgg ggcctgggaa gagctacgtc tctgcgccga ccacgctcaa aaaatacaag
     6961 aacggctatt acgtcaaccc gatcttccca cgctggagca tggaaaagtg gatcgagtcg
     7021 gggcagccgc atgcacagca gaaattgcgc gagtacacca tcgaattttt gaaagacctg
     7081 cccgcacccg aagattacga agagttgcat gccaaaggtg aagagtttat cgcaagccgt
     7141 ttgagtaaat aaagtgatgc ccgccagagt cgccaccttt gtttttggaa tcgtgtttgc
     7201 cgtgttattt tcatggctgc ctgtttaccc gttgctaatt gaaggccgtg agatttcgga
     7261 atctggcgaa agcttgtatc aggaatggcg ctttgtccct ctggctgaat ataacgactc
     7321 ggctatcttt gcccgttctg cgtggcagaa gtctgtccgc gccacatatg ttgtgacaac
     7381 gatcgtcaat tatcttgttt tgtttggggc ttcttgggga atcgcttccc tgggtgtgcg
     7441 catcatcaaa aagagcgcgc caaggcttat cgcctgacgc gctcttccaa tgcttgctgg
     7501 tttctttcct ttttactttc taaactttcc ccacaatcca tacccttcat ttcttctctt
     7561 ctcgcgccgc tcatgttgct cgtggatttg ctccacatga tcggcttttt cttcgatggc
     7621 ggcttccagc cataggcggc ctttttcagc cgtggcatga ctgcccgcgc cgtatgcgcc
     7681 tgtcttggtg tcatcgtccc acggcgggtt ggcgaccaaa gacccgcctt caatccaatc
     7741 catgtaatag tcgggtgtgg agacgaagtc tgtttcatcc accacgcgat ccatctggca
     7801 gagatcgggg cgcaagtgca gcaggtacga tgtttccaat tcgcctgcgt ggcccatgcc
     7861 gccgattttc gatgtgcgat atttatcgag cgcctccgcg ccgatacggc ctgaggtatg
     7921 caaaaatgca gtggcgcaga aaatgcggcg gtgacgttct ccagcgtatt tgacgatgtt
     7981 caccaaaaac gaagtgttgc cgccgtgtcc gctcattaag tagaagcggt caaaaccgcg
     8041 cgcgacgaga acatcaatgg cagcgcccca aaaatcttca aaggcacgtc cgcctgcgtt
     8101 caaagtggcc gcaaaggatg aacgatgtgt gctgactcca taaggcatga caggcatcgc
     8161 ccaactgcgc gtaggcactt tggctgctgt gccttgggca attgaattga taataatggt
     8221 gtcaacggaa agagggaggt ggtagccatg ctgttctgtg tgaccgatgg gaatcaagat
     8281 cacttttcca cgttcgctat ctggtggaag aaaagtttgt gcctcataaa gtgacggatg
     8341 cggctggcga agaatggatg cagaatgttg aagaatgaag ctcgattgcg ggtcaaggcc
     8401 ttgtggcgcg aggcaataga cccgtgaaaa tccatcatcg cgcagactat ccatgaggtt
     8461 gacgatgtac tgcgtgaaga tcgcatctgg caattcaagt ccgctgccac gccagccgaa
     8521 tgggaacggt ggcaggagtc cgatgcgggg cggattgctt agttggtcag caagcaggtc
     8581 gaggttatat ccagagccga gtgggaggat gagcggcgtg tcgcgtggaa gttccgcaac
     8641 cgcatcccaa gtcagttcct cgtacaaaaa gaactctgaa tttcgtgtag ccaataaaga
     8701 acgattattc attttaattt ttcctctcta tttaagaagg aagtaaatac caatcaagga
     8761 atcgttccat gcgttcgtaa acatcgagca gattctcgcg gcgtaaaaag ccatgcggct
     8821 cgtcgccata ggttttgtat tcgaaggtct tcccttcgcg gcgcagggct tcaacccatt
     8881 cttcgctggc ttcaggtggc acaatgctat cgagcaggcc atgcaaaata agcacgggtt
     8941 tttgcacatt ctttgcctcg gtgatggggg agcccttgag atagatttgt aaattttcag
     9001 atggatggcc gaggaaaatt tcggtataaa cccgcaggcg tttttcacat tgcgcccacg
     9061 aactgaccag atttgaatcg ccgtacttgg tgatgccgca ggcaaaaaga tattcggggt
     9121 cacgcgaaag tgagcagatg gtcatgtagc cgccataact tccgcccgta atggcgatgc
     9181 ggtcgggttt gatggttttg agcgtgcgta aatatttggc gccatgcaaa caatcctgcg
     9241 tgtcgccgat gccccagtcg ttgtaattgg cgcgctcgaa ttcctggccg taccctgtgg
     9301 agccgcgata attcggcgca agataggtat agcctttcgc aacgtaatac tgtgcgagtt
     9361 catcccagcc aaacccatat tggtcctttg gcccgccatg cggatacaaa attgcagcgc
     9421 cgttcgattt ttcagggcgg tataaaaatg ctgggatttc cagcccgtcg tagcttttgt
     9481 atgaaaccac ttcaggcacg atgaatttat ttctctgcaa cgcaggcgga tttgaaaaag
     9541 taagttgtgt gattttcttg ctcggaagct ccatgcgata cacatcgggc ggaagggtcg
     9601 gcccttcgaa ctcgaacgtg atgaaggatt cgtccttcgc ccagtttgga ttcgagtgaa
     9661 tgcccttttc gcttcttaat gcggaccttg agcctgattc tgtctcgagc agcattaact
     9721 caaacgatcc gccattgttc tccactacga gcatctgttt tcccgaaggt gaccactcgt
     9781 attggaaaat atcttttcca gccttggtga gttgatgcag cccttcgcca tctggcttga
     9841 tgagatacag ttcttcgcgt ccagtttcct gcgagatgaa cgaaatccat ttgccatcgg
     9901 gtgaccattt cggcgatacc gcacgcgtgg acggttttcc gtacaacgtg gtttgtttgc
     9961 ccgtggcaat ttcaagcagg acgatgtcga ggcggttcag gtcgtcaaag cggcgcaggt
    10021 tgaagacgat gaattttcca tcgggcgagg ggcgcgcatc ccagtgatcg ccgatggtgt
    10081 cggtggtgag cgcgcgtggc caatctccgt tgtaattggt aagcagtaat tgatctgctt
    10141 cgtttctttc gacagtgacg atcaaaccga tcgagtcggg catccagcgt ggtccgctgg
    10201 cggctacggc gaaatcggtg atctttttgg gaagcccgcc atcgcgagga acaatgtgga
    10261 cgtggccttt aattccaaat gccagccaat tgccatcggg tgaccattcg ggaatctcat
    10321 catcccaata tggaacaagc ggccgttcgg tggaaattct ctgcagccaa ctgccgttcg
    10381 cctgcatggt gtagacatcg gaaaggcttt cgccgtcttt gatgcaggag atctgtccct
    10441 gagatgaaag cgcgtgagac cgaatccgct cgagggaagt gagaagcgag aggtttaatc
    10501 cagcaggtgg attcaggtct gggcgtttga ccgagggcca gccgttccgt tcatattttt
    10561 cagtgagttt gaccgagttg tccatggtgg tttcctttcc atttcgtgcc aagtatagca
    10621 aaataggctg aattacagta caatttttac aggatacgct tttggactct ctggaatgtc
    10681 tctacttttt tagcacgttg gcccgatgag tacccgcgtc aaggcaggtt tctgctttga
    10741 cgcgttttgt tatcataatc aaccaaagag gagagaatat gaaacgaaga tttctgttta
    10801 ttctggtcat tctaacgctg gttctttcag cttgcggagg cggaaatgag aaggccattg
    10861 tccgcattgg gtgggcaggc agccccgata atctcaaccc aggcatggga attttgaccg
    10921 aatcatatac gatctacaac ctggtctacg acacgatgta cagccttaat ctcgatggtt
    10981 cgttcaccct gagcattgcc gacagcgtgg agcgttctga cgatggtttg gtatacacct
    11041 acaagatcaa ggatggcatc aaatggcacg acggcaagcc gttgaccgcc gaagatgtgg
    11101 ccttcagcta caacctgtat aaagacaccc ccgaatatcc ctacctgaac ggttattaca
    11161 cgacctattt cgcttctgta gaatccaagg gaaatgaagt cattcttaca ttgaccgagg
    11221 caattcccaa tattgaaagt caattggtct ttctttacat tttgcccaaa catgtttggg
    11281 aaggtgtgaa taagctggag tatgaaaact ttgaaatgat cggctccggg ccgttcaaga
    11341 tggccgaata tgcccaaaac gaatttgtgc gtctcacggc aaacaaagaa catttctcca
    11401 ggccgccgaa ggtggatgag gttgtcttcc agacctttga aaatcaagat gcgttggtac
    11461 agggtctcaa gaccggtcag ctggatatga tcacagaaat gccgaacact tctgtggaaa
    11521 ctttgaaggc tgaagagaat gtggaagttg tgatcggtgc gccgttctct ccaggcgtga
    11581 ccgacatcat tttcaaccag atcgacccgg ataactgtcc caccgatgcc ggcggtcttt
    11641 gcacgggcca ccctgccttg cgtgaccgca acgtccgcct tgcgatggca catgccaccg
    11701 acaaacagaa attgatcgat ctgatcctgc ttggccttgg tacgcccggc ctcacgctca
    11761 tccctgacgg gcttggtgtt tggtacaaca acagcatcaa agattatgaa tacgatgtgg
    11821 ctgctgcaaa caagatcctt gacgatgcag gctacaagga taccgatggc gacaagatcc
    11881 gagagatgcc agatggctcg aagccgttga ccttccgctt aaactggccc agcgacagca
    11941 tcaacgctcc gcgcatggct gagttgttga gtgaaatgtg gggcgagatc ggtatcagcc
    12001 tcgaaatgca ggccgttgac cccgatgcgt tgaccgccca gtgctgccct gccttcgact
    12061 ttgacatcat gatctggggt tggggctcag acccagaccc aagcgccttg ctttatgttt
    12121 acacttcgga aggcattccg acgggttcaa gcgaaactgg atactccaac ccgaaatatg
    12181 acgagcttta cgctcaacag caaaccgaat tgaactttga ggcgcgcaag agcattgttt
    12241 gggaaatgca gaagttggtt cacgatgacg tggtgtacat cattcccttc tatgatgccg
    12301 aaactcaagc ctatcgctcc gaccgcttca ccggctggat caccaaccag cccaaggtgg
    12361 aactttcaga cgtaacctct ctagtgctga tcgagcctgt taaataaacc ccacagcagg
    12421 gagcggaaga tgccttccgc tccctgctca tgttttcaag gagttacctt gaaccgttat
    12481 cagatccttt tacgaaaaac agcctgggcg ttattcacca ttctgttcgt tattatcctg
    12541 aatttctttt tattccgcat cctgcccggt gacccggcgc gggcgggaat ccgtgacccg
    12601 cgtttgaaga aggaatccat tgagattttg cgggtccgtt ttgggttgga caagcccatt
    12661 atcaactgct ttgagtcgct gaatccgatc aaagtgggga gttgtgaggt taatccgctt
    12721 gaaacgcagt tctttattta tgttcgcaat ctgttgcagg gcgaattcgg gtttagttat
    12781 cacacaaacc gccctgtggg cgaaattcta gccgagcgtt tgtggaatac ggtcctgttg
    12841 atcggtgcag ggcagatttt gtccatcctt atcggggtgg cctttggcat tattgcagcc
    12901 tggaaggcac gcacaacggt tgaccatgct gcgattacct tcagcctggt ggcatggagc
    12961 cttccaacgt tctggctggg cattatcctg ctgttttggg gcagtcaaaa cggtttgccg
    13021 ctggcgggaa aatccacacc gggtatgagt tccatgccgc ttttgcagca atggggcgat
    13081 ctcttcgtcc acatgttcct gccaaccctc acctatacct tgatttacat ggcggaatat
    13141 acactcatca tgcgctcaag tttgatggat gtcctttcag aagattatat tttgacggcg
    13201 aaggccaaag gcttgagcaa ttttcaaata ttgaaggacc atgccttgaa gaatgcgatg
    13261 ctgccactgg tgacggtcat cgccatcaac cttggcttta cggtggcagg tgccgtccag
    13321 atcgagacgg tcttctcctg gcccgggctt ggcagcgcca tctttgaagc ggtcaaccgt
    13381 cgtgattttc ccgttctgca aggagccttc ctgttgatcg ctgtcgccgt gatccttgcc
    13441 aatttccttg cggatctcac ctacacctat cttgatccgc gtgtgaccat ggagtagcct
    13501 gatggaaacc acagtacagg ttgttgaaac aaagagtcgt ttccatgggt tctggcgaag
    13561 cctccgcaag aatcggatcg gattcatcgg gttggtgatg ctggctgtca tcgttttgct
    13621 ggcgcttttt gcccctttta ttgccacaca tgattcatct tcttcagctt cggctgagat
    13681 cggcgacatc tacaatccgc ccagtgcaga gcactggctc ggcaccgatg atgcaggtca
    13741 ggatgtcttt tcgaatttca tttttggggc gcgtatttcg ctgatcgtgg ggttctttgc
    13801 cgcttttatc tccctgatca tcggggggat gcttgggttg gtcgctggct tttttggcgg
    13861 gcgggtggat aatgtcatca tgcgctttac cgatatcatg ctcgtcatcc ccgacctgcc
    13921 gctgatcgtg gtgatcgtag cgttgaccaa gccctcgctg cttaacatta tctttgtcat
    13981 cggtttgctg gggtggacga caaccgcacg cgtggtccgc tcacaaacat tggctgtaaa
    14041 atcacggaaa tttgtgctgc gggctcgtgc cattggcgca ggcagaagac acatcatcac
    14101 gaaccacatt ctgccgctgg tgatgcccat ccttgttgtg caggctgtgc ttgtagtttc
    14161 gagcgccatc cttagcgaaa gcgcgctttc gtttctgggg cttggtgacc cttccgccct
    14221 ttcatggggt caaatgctga actacgcttt tggacgtggt gccatgtcca ttggtgcgtg
    14281 gtgggccttg tttgtgcctg ggttcggcat cgtgtgggtg gtgcttggtc ttaccctgct
    14341 tgggcaggga cttgagcagg ttttaaaccc aaggttagac actcatcatt taacaccggg
    14401 caggcccacg gtccagcatg aagcggcgca gccttccgaa caaaaatcag aaaaattgct
    14461 cgaagttagg aatctttcgg tcaattacgt caacgaaggc aagccgccag cccgggctgt
    14521 agagaatgta agttttaccc tgaagccagg tgaattgatc gggttggtgg gagaaagcgg
    14581 ctgcggaaag acaacgctga tgcttgcctt gttgcgcctg cttccatctg cggggcaggt
    14641 ggtgaatggc agtgttaatt tccacggcag aaacctgacc gagcttggcg atgatgaatt
    14701 gaatcaagtc cgctggaagg gaatttcaat tgtttttcaa ggtgcgatga acgcgttgaa
    14761 tcccgtccgc acggttgggg aacagatcgc ggaagccatt atcaagcact tgccagattt
    14821 tccgcgtgat gcacgtttgc aggaacgtgt ggtggaactg ctggatctgg tgggcattgc
    14881 ggcagatcac aaagaccact ttccacacca gtattcgggc ggtatgcgcc agcgtgcgat
    14941 gatcgccatg gcactggcct gcaacccgca agtgatcatt gccgacgaac ccaccaccgc
    15001 gctggatgtg atgatccagg ctcagatcct tgagctgctt gattcactgc gcaagaaact
    15061 tgggcttgcc attatttttg taacccatga cctcggcgtt gttgctgaaa tttgcgataa
    15121 agtgctggtg atgtatggcg gtgttacggc cgaatatgcc gatgtggata cgatctacaa
    15181 tgagccgcgc cacccctaca cacaggaact gatcaaagcc tttccagacc tgaccaaacc
    15241 gaagaagaaa ctttcatcca ttcccgggta cccgcctcgt ttggatgacc tccctgccgg
    15301 ctgccgtttt gcgccgcgtt gtccgcaggc ttttgaccgc tgccgcaccg aacaacccgt
    15361 tctgtacacc gtcaaggaag accatgtgac aagctgccat cttgttgagg cctcaaaatg
    15421 agcgatgacc aatttctaaa agttcgtgga ttgaaaaaat attttgatgc aggccgtcct
    15481 ggcttgttat ttggcagccg aaccccgcgt tacgtccatg cggtggatgg cgtggatttt
    15541 acgctcaggc gcagtgaagt tctggctttg gtcggcgaaa gcggttgcgg gaaatccacc
    15601 ctttcgctgc tgttgatggg tctcgaagac cccactgaag ggaacatcat ctttgagggc
    15661 cgcgacatca cccacctgca agacagcgaa cgcaaggaac tgcgccgcaa gattcaaatg
    15721 gtgtttcaag acccgtatga atccttgaac cccacgcaaa ccatcgaaga gatcgtcacc
    15781 gagccgttga tcgttcacaa cctggagacc gaaaaactcg aacgtgatag ccgcgtaaaa
    15841 aaggcactgg aagatgcagg cctgaaacct gccgaagtgt atttgaaccg tttcccgcac
    15901 gagctttcag gcggacagcg ccagcgtgtg gtgatcgctg ctgcgcttgt gcttgagcct
    15961 gaaattttgc tggcagatga acccgtttcg atgctggatg tctccatccg tgccgagatc
    16021 atcaacctgc tggccgaact ccgcatcaca cgccagatcg ccgtcatttt catcacacac
    16081 gaccttggct cggttggttt ctttgccgac cgtgtggccg tcatgtacct tggccgcatt
    16141 gttgagatcg gcaccatgct tgaggtactt gaaaaacccc aacaccccta cacaaaagcc
    16201 ctgctttccg tcatcccggt gccaaatccg cgaatgcgcc gtgaacgcat catcctgcaa
    16261 ggcgaaaccc caaaccccat tgacctgccc acgggctgtc gctttcatcc acgctgcccc
    16321 tcggccatgc ccacttgcaa ggtcagtgac ccgccattcg aggtgttcac gaaaactcat
    16381 caggccgcct gcctgctttt ggtaaagtag ggtcggcgct gcatcaaaac atcatggaca
    16441 tcaccagttt acaaaaccca aaaatcaaat acatcgtcaa attgcgcgag gataaacgtc
    16501 tgaggcgcaa ggatgggtta atgcttgtgg aaggccgcga tgagttgact ctggccctca
    16561 gcactggact caagccgcat acgcttctca ccgcgcccga gcttgccact gttccaatgg
    16621 atatttcctc cgctgagatc accaccgtca cccgcgctgt ctttgagaaa atttcctatc
    16681 gcgataaccc cgatggctgg ctggggcttt ttcccattcc acaaaccgat ctggatgatc
    16741 tgaaattggg cgcaaatccg tttgtgatcg tcgctgaatc ggtggagaag cctggtaacc
    16801 ttggcgcaat tctgcgcaca gccgatgcag caggtgtgga cgcggtcatc gtttgcgacg
    16861 ggcgcgtgga tgtgtggaat ccaaatacgg tccgcgccag ccgagggacg gttttcaccg
    16921 tacccaccgt cgaggctgat tcgcaaatga cgctgaactg gttgaggtta agaaagatgc
    16981 gcgtgcttgc agcaacacct tctgccgagg tgctgcacac ttccatagac ttgagtcaac
    17041 ccatcgcgtt ggcagtcggc acggaagatg aaggtctcac cgatttttgg atgcaaaatg
    17101 cggatatcaa agttaaaatt cccatgctgg gaaaggtcaa ttcgttaaat gtttcgatcg
    17161 cgactgcgct catcacatat gaagcagtgc ggcaaagaaa tggtaaatcg tgagaggtaa
    17221 ttcgatgaag gcattgtggc tggaagacaa tcaaatttca ttgcgcgatg tggcaaaacc
    17281 aaaaaatcca aatgaagcac tgatcaagat ccgcaaggcg ggaatttgca gtaccgacct
    17341 tgaattggtg aagggatatt atccatacac aggcattctt ggccatgaat ttgttggcga
    17401 ggtggtggaa gcccccgacg ccgcatggat cgggcagcgg gtggtgggtg agatcaacgc
    17461 agtatgtgga acctgcgaaa cctgcctgag cgggcgccca acccattgtg agaaccgcac
    17521 cgtgttgggc atcgtcaacc gtgatggcgt gctcgccgaa tatgccaccc tgcctttgaa
    17581 caatctccac cgcgtccctg attcggtttc ggatgatatg gccgtgttta ccgagccact
    17641 ggcggcggct ttggaaattc aacagcagat ccagcttagg cccgatgacc gtgtgctggt
    17701 ggttggcgca ggcaggctgg gacagttgat cgcgcagacg ctggcgttaa cgggttgtga
    17761 cttgcaagtg gtgacccgcc atgctcatcc tcaaagtttg ttggctgcac gcggaattcg
    17821 attcaccaca gtcgatgagg tccagccgag aaaatgggat gtggtggtgg aagtgacagg
    17881 ttcaccagat gggttcaacc ttgcccgcaa agccatccgt ccgcgcggga cattggtcat
    17941 gaaatccacc tacaagggtg atatgaatgt gaatttctcg tccattgtgg tggacgaaat
    18001 tcagatcgtt gggtcacgct gtggtccgtt tgagccagcc ctgcgtttga tggaaaagcg
    18061 cgaagtggac cctacagttt tgatcatgga acaatataaa ctaaacgatg tcgttcgggg
    18121 atttgagcac gccgcaaggc ctggagtact gaaagtgctg atcgaaatgt gataaggaat
    18181 ttctttagaa aagccatttt gaaccacgga gacactgaag ctccgtggtt atttttcggg
    18241 cttgtgcaat aagttttcag gaatcatttt gttttggtgg gttattgaat gaaaaagtta
    18301 aggatgaatt gcagtaacag ccctgcaaaa ggaagcagga ccagagcgag cgcgattcct
    18361 gcttgagatc gcgagtcgtt cttcctgcgc cagccgagga ctgccagcac aaaagccagc
    18421 ggggtgagaa tgcaaaagaa caacgtggtg atctgattca agatgccaaa ggtgcgcggg
    18481 gcaatatcca tgtatgcgac agcgaagcgt gccgccaaat caaggacgga tattccgcca
    18541 atgatgagcg cggccaggcc aaaataggtt cttttttctc gaagtgtgga catgaatcgg
    18601 attataaacc agaccctgct gaaagaatcg ataccagcca aagataaacc gacccattga
    18661 tcattccatg agccagcatg gcgggggcaa tggagcgcga gcgcaaggtc agccagccga
    18721 agagcaaggc tgcggcaggg atgaacaccc aaaacatgaa tggatattgc ccagcaaaat
    18781 aaacatgggc ggaagtgaaa agaacggctt gaaacaccaa tatccaacca tccttccatt
    18841 tgagttggcg cagggctgcc cataaaaatc cgcgaaagag gggttcttca ttgataggcg
    18901 caaagccaag gtgataaaac aaattcaggc tggtggaaag caggacggca gatgaaaatg
    18961 cccgtggatg tgtcaccatg gactggaagg atgaaaaatt ttcagcgatg gatgttacgg
    19021 cgccgatcaa gagtccgaag ctgagccagg ccagggttaa gaaagagatg cgtgtggatt
    19081 tgaatccact ttgccaaagg gcaagcccca gaccgattgc gataatccaa atcaacaggc
    19141 tgggccatcc agggaaaacg agcggagttt ccaccttcca gtatgcgagg atgagagttt
    19201 ggatcggtct gagaaaaatg atgagcaaca aggctgttgt gtcgatgtgc aggttggcga
    19261 ggttttcgcg ctcccaccaa atcaggaagg cggtcagaaa ataggtgccg acttcataga
    19321 ccgcggcgcc attttgattt tcaattggca ggaagtaaat gatggcaagg gtgtaaggga
    19381 ttctaagaaa aaggagcagg ccgagcacac tccaaaagat ttgacgatgt cggcctgctg
    19441 acggtgtttc aagcatggct tagcgttgtt ttgtccaggc gatcattggg tcaatccttt
    19501 ttgcgaggcg ataggcggca tctttgaaac cgaagcgggg ccagaaacgg gaagcaggca
    19561 ggttgggact gatccagttg gtgtagcaaa tattgaagcc ctctttttga acctgtttca
    19621 atccgcaaag agtgagcgca gtgctgatgc cgcgtccgcg agcttgcgga agggtggcgg
    19681 caatactcaa gtagacagtg cgtggggata ccaacatcgc agtctcttgc tcttcttcag
    19741 gacggaagcc cacggtggca agtgcttttt ctttttcaag agccagccat acggtccact
    19801 cctgatcgtc tgccaattca gcccatcctt cacgcagttc ttcccaatct tctgggatgg
    19861 ttggatgcca atagggagca tttgccaatg attccatgat gacatgtgaa agatcgccaa
    19921 ggtgggaatt gtcacctgtg cccgcaattc gaatatcaat gttggctggg gctgctggct
    19981 ctgcaatttc tgcgatgcct agatccagca aggcatccac gcgttccttg ccgaaaccaa
    20041 gcgcaaacaa ggcgtcaatt acatctgcat ctgcggctga aatataaagg ctgtgactaa
    20101 agacaccttt ctgtacccaa tcttctccca atagagaata taggtcttga agtgtcttca
    20161 ggctttcacc ttcctccaat gcatacccag ccagatacac atatccgcag cggccccaag
    20221 gttgaacggt gaaatcaccg atcaagtatg cggtcatctt tccatcgcgg aatgcggcgt
    20281 atccattctt gaatttcttc tgccaaaggg cttccactgc cttgcctgca actgttgagt
    20341 cttcgaatcg ttcaggaagc aaggatagtt tggcgcggtt tcgcttgtga cgcgcagaaa
    20401 gcaaattccc tgccagggga atcatctctg ctgaaaatgg cttgatctcg gttttcatgt
    20461 ttcaatttcc aattctgggt tttcatacga aaagcaggtc aggatgcaaa ccctgacctg
    20521 cttcactaaa tttacttaat ctgcggcaat ggcttcttcg ccagccgttt cacgttcgcg
    20581 cctgaccatg atcgaatctg cggttacatt ggcacgttcg ttgccgtcaa tttcttcggt
    20641 gacaccatag gccacttcca attcatggcg gaactgctgt gtgtacaggc ggtaataatg
    20701 tccgcgctgg cggagcaact cagcatgggt gccttgctcg gcaatgctgc cgttttcgat
    20761 gacgagaatc cgattcgcgc ggcgaatggt ggaaagacga tgggcaatga cgaaagatgt
    20821 gcgccccttc atcaaggcct ccatgcctcg ctggatgagc gactcggtca gtgtatccac
    20881 ggaggaggtc gcctcatcca tgataaacaa ctcaggtctg gcaagtacgg cacgtgccag
    20941 cgagatcaac tgcttctgtc caacggagag caggtttccg ctttcaccta cgttctgctc
    21001 gtaccctttt tcaagcgtca caatgaaatc gtgagcgcct gcgatctttg cagcttcttc
    21061 cacttcggca tccgtggcag acaaattgcc atagcggatg ttctcgcgaa cggtgcctga
    21121 aaacaggtgc ggggtttgca gcacgatgcc gatcttgttg tggatcgact cgagcgtgta
    21181 atccatgtag tcgtgtccgt tgatgcggat gactccattc cgcggttcgt aaaatcggca
    21241 gagcaaattc acgatcgtgg acttgccgcc gcccgtcggg ccgaccagtg cgatcatttc
    21301 gccaggcttc accttcagcg tgaaatcggt caatacggat tttctgtctt catagtaaaa
    21361 atccacgtga tcgaattcga tctcaccgag cagagtcttc gcttcgatgg catcgcttct
    21421 attatggacc tcgggcgggg tatccactaa tttgaaaatt ctctccgctg aggcaatgct
    21481 gtgctgcatt tcggcataca cgcgcgccag gtcctgtaca ggccacatca tgaaagtgag
    21541 atacgaaacg aaagcctgaa taccaccgat ggtaatgagc cctacttgaa tttggactcc
    21601 accgtaccaa acaatggcac ccagcgccaa cgcagcgatg atctgcacag tggggaggaa
    21661 gagcgccgag agccaggctg cacggtagga agcgcggtac attctcgtgg tcaactgctg
    21721 gaattccacc agattttcgt cttcgcgtcc cagcgccttg accacacgta cgccctgaat
    21781 attttcattg tacgcgcctg tgattttgct gttggcgcgg cggctctggc ggaactccac
    21841 cagaatgtat ttctgaaact tcgttgccac gaacatcatg atggggatga tgaccgaaac
    21901 gatcaacgcc agcctccagt tgatgatggc catgaaggtc aacgaggtcg taatgttcat
    21961 cactgcccaa acgctgtcca cgatgcccca ggtcaccagg tcagagacac ggccagtgtc
    22021 tgaagttaca cgcgcgataa gtcgtcccac tgcattttgg gcgtagtagg aaagcgatag
    22081 gtcctgcagg tgattgaaga gcattttgcg caggtcatat tgcacccgct cgcccagcac
    22141 acctgcaagg taaatgaaaa tgaagatgaa cgccgcctgc aagatcagga acgagccgta
    22201 catccatgcg atttcgctca cgcgctcgta atcactgaac ttgataccct gatcaacgat
    22261 ctgcttgttg aggtaggtga aataagcgtc catgctggag acaagggcaa tggttaccaa
    22321 aaagcccagc acccatctcc aatgcggctt cagcagaccg ccgatccgtc tcagaacggg
    22381 catggtcagg tttgatgtgt attcttcttc ttcgagttcg atcaattcgt cagtcatggt
    22441 aattctccag aataaaatga attcagcggg agttgtccgt caaaattcat aattcagatt
    22501 tcataatttc ctgctcgagt tcttcgtcaa ttctggtctg aatgtcgtac acctttcggt
    22561 acatgccgcc aaattgtttg agcagttctt cgtggttccc tttttggatc acctgacctt
    22621 tatcgagcac caggatcaag tcggctttca tcacagactg gatgcggtgc gcaatgatga
    22681 aggtcgtgcg gttttccatc aggccattca acgcattgcg gatggaggct tcagtctctg
    22741 tatctacgga ggaagttgag tcatccagga tcaaaatgcg cggattcttc aggatggtgc
    22801 gggcaatggt cacacgctgt ttttgtcctc cagagagggt cacgcccttt tcaccgacaa
    22861 tggtgttgta gccatcggga aaggtcagga tgacatcatg tacggcggca gcttttgcgg
    22921 cggcttcaac ttcttcctgt gtcacactgc ggcccacgcc atacagaatg ttctcgcgga
    22981 tcgagcggct gaacaggaac ggttcttgtt gaacgatgcc gatctgttca cgcaggtact
    23041 tgcgtggata ctctttcaat tccacgccgt caagcaggat ctgcccgctg gtgtaatcgt
    23101 ggaagcgcgg cagcaaattc accagtgatg tcttgcctga tccagtggaa cccagcaggg
    23161 caatggactg tccaggtttg atatggaagg aaatatcctt gatcacatca tgcgtgccgt
    23221 ctgagtaaat aaacccaacg ttcttgaact cgatctcgcc tcgtgccggg cctgcgggct
    23281 ggatgctgcc atcggtgaga ggttcccgcg cctgtttggt gatgtccatt aagcgggcgt
    23341 aggagaccat ccctgtcgat gtctgcacaa tggtgcgccc aagatttcgg attgggaaaa
    23401 taagccacac cacaagccct acatacgcca cataatcacc gactgttatc tcgccgttga
    23461 tggccatgaa ggcggcgtac acaaacccaa acaacatttg ggcgccaagc acgatatccg
    23521 aaaggggcca gaagagtgag tgcatgagca ggagcaattt acccttcagg tatttgcccc
    23581 agttgtcttt ttcaaacttc tctttttcgt agtcctgccg tgcaaaggct ttcaccacac
    23641 ggactcctgt caggttttcc tgcagggtgg tggagagcag tgcttcctgc gcctggtaat
    23701 cttcgtatgc cttggtcact tttttgaaga accaaagcga aacccacaag atcggtggaa
    23761 taaccaccac agaaagcaag ccgagtttga cgttgatgta caggatcgca acccagttga
    23821 tgacaaacaa caggatgatc cttccaacac caatggcctg ctcggaaaag aagcggcgca
    23881 aggcgtccac atcggaggtg acgcgttcga tcaagtcacc ggtcggtgtg gcgctgtgat
    23941 atgaaaagct caagcgctgg atgtggtcga agaggaagtc tctcaaacgg cgggtaatgc
    24001 cctcggcggt gtaggcagcc aaacgtccgg aaagaaacgc aaatccgcct tcgaaaagcg
    24061 ccaggatgaa gaatcccaac ccgatgtaaa ggaaggtttg ggtcatcgtc gttccgatca
    24121 ttgtgccttt tttcaagaca tcgtctgcaa agaagcgcaa caaaagatag acggatgttt
    24181 tcgccagagc tgagactgcc agggccaaag ttgccgcaac gtaggacaaa cggaaatccg
    24241 tcatcatgcg ccatagaccg acaaggcggt tcgtatgcag tgcatcgcgg aagtcaaaat
    24301 atggggaggt tgtagtggtg gacatgtgtg gttctccgat aaaaagggaa acaaaaaggc
    24361 tgtggtgagt gcaccacagc ccataaacga acaagggaca gaaagagacc tagcggcggt
    24421 ctgcaggcgc actccgagaa ggcaaaatgc caacggcttc ggggaaggtg tagaaaatgg
    24481 tgttgaacat caagagtgcg ctcctttctt tggatttttc gtactgtgag gctgagttta
    24541 cacctttgat ttgagatgtg tcaaggaatt tgtgaaaatt gtttgcaggt ttcatgggat
    24601 gattctaaga aatgcctgct tacgacgcag atggcttggt ctctgctggt atctgtgcat
    24661 ttcttttttg aacgttgcgt gttgccatca aggttaatcc gatcatgacg gggtaaatgg
    24721 cgataaagcc aagggtcagg attgttttaa cccgcctttt tgtttcctcg acaaggttgg
    24781 catcgaaatc ggcacacaaa gcgccaactg attcgccatt gtcattgtaa atgggcgcgc
    24841 atgaacacac ccagccgcca ttctcatttt ggtaaatggt ctgtggatct gccaccgttt
    24901 cgttcatgcc gacagcgtgg atatctgacg ggttattgct ggtgatcgaa aatcggaagg
    24961 ggaggtcttc catggtggaa acaagcacag aaaactcccc ggaattccct ccctttacga
    25021 tcgtgtacag tacgatctct ccgggaatgt ttgtgttgcc tgccctgatg ccatccacat
    25081 agcgggtgag cctttgataa tactcatcgc tgtacacggc tgtgtcagat gggtcggcat
    25141 atgcgctggc aaacgattga tattcatttc cgtccacgat gcttacgctc gtttccacca
    25201 atgcaagcat ctggttctta atggaattca gcgccaggtc tgtggtgacg cggcttagcc
    25261 aggcatagag cgcaaagaag gcgagagtaa aaacagccaa caccaggaag gtaactcggc
    25321 gcaaacgcag acgcatttcc tgctcaagtt taagttgttc cgcctccgcc cgttctgctt
    25381 cgataaactc attttctgcc actcgactcg ctgaaatata ggtgcgctgc aggctggtgg
    25441 ggaggggttg cttttccccg ttgaccttca actgcgcctc ggcatccagc aactccatgc
    25501 catgcagtag aaaactgtcc tcgcgcttgt tgcgatccca ttcaagagcc tttacctgca
    25561 ggcggttatg ggtttgcacc caatcgtaat cggtgcggat ggcggtcagc agtttattga
    25621 atgcttcctc aaatgtatct ggaggccgtg aaaagaagac ccaattcaaa tgtgagagcg
    25681 acttcggtgc atccttcatg tcgatttcgc gcgcaaccac aggtgcgata cgcttgccgt
    25741 ttttgatcgc atgggccact tcatctgtgc acacagggga aaggatcgaa tccgggctga
    25801 cgacaaaaat aaaaacatcc gcctcttcga tgcctttttc gatttcttta aaccaatcca
    25861 cactgggagg aatatcctgc caatcgatcc acacgctcat tttttcagac tcaagactgt
    25921 ttgtaagctt gcgggcaaaa tcggcatctt ttcttgaata ggaaacaaaa agctggttca
    25981 taacattaaa ttccttccat ttgacttgca agattgtgga tccacttacg cgaccgccag
    26041 cggaaaattc ccaggatcca tttggtcact tcctcgctca ttacgcagag atagacaaaa
    26101 tagacaggca aatgaaaata aaaagcgccc agtgccgcaa aaggtacgcc caccagccag
    26161 atgataatcc catctaaaaa cagggagaag cgtgtatcgc cgccagcacg cagaatgccc
    26221 acgacaatgg tcatgttgtt gacccgcacc caaaggaaaa tggaaacgat attgaccatc
    26281 acactggcat tttgcagaac ctctgaagat acattgaaca gggaaagcgc ggggactttg
    26341 aatatctgca acagaagccc cagcagcact ccgccggcaa tgccaaggcc gatcgaacgg
    26401 ccgccataca gataggcttc atcgtccttg cctgcgccga tgcggttgcc caccaaaacc
    26461 gaggttgcat ttgccagccc tacgaagacc acaaacgcta tttgttcaat gggcgaaaca
    26521 atattgaccg tggcaagcgc ctccgtgccc atccgcccgt agatggcatt gtaggttgtg
    26581 attcccagcg accagaacaa ctcattcagc attacaggca gcatgggttt gatcacacgg
    26641 gcggtgaaag ccaggtcaaa atcggtcagt tccttgatcg aggccgccac agggaattta
    26701 taggcataga tcacatacaa aaggatcacg cactccagag cacgggcgat cactgccgcc
    26761 accgccgccc cttgaacgcc catttctggc atcccaaaat acccaaagat caacacatac
    26821 gaaagaagcg tgttcaaaga cagaactccc acgctgacgt aagtggggag tttgacactg
    26881 cccgtggaac gcatcacaaa aacataactg gcagaaaccg ccatgaataa aaaggtccac
    26941 gaaaaagtgc ggacatattc cgtccccatg gcgatcacct ctgggtcttt cgagtaaatc
    27001 cccaaaaacc attcagggac aaattgcgag atcgcaaaaa atacagcgct ggctgagatc
    27061 gcgaacatca aacacaatcc caacacacgc ttgaggtttg gaatatcctg cctgccccaa
    27121 aattgagcgg tgaacatggc cgcgccgctg ataattccaa agtggaccag attcaaaagg
    27181 aaagcgatct ggcctgccag ccccaccgcc gccacagccg tatcgccttt ttgccctaca
    27241 aagaccacgc ccaacatatt caacaaggca tacacaaact gctgaaaaat gatcggcagc
    27301 gcgatcttga aaacattgtt gaagtattca cgatcactat ataaagctac gattttattg
    27361 aacacatcac ctcaaggttt gccattatag cagtaagaga cccttcaaac cgaccatcta
    27421 aaagcccaaa ggagaaccaa tgaaacgact gttgatttta ttgatatttg tgatcaccgc
    27481 ctgcacacct gctttcgtag atataacccc gactcccgct tcaccgttgc ctgatgcgtc
    27541 cccaacagaa acaacctccc cgcaagtgga ttcagccacc aacacccccg ttgaacttga
    27601 atccaatgtg gatattcttg ccaacctccc caaccccgat tgcgccgctc tcacccctgc
    27661 agaccaggaa gggccttatt acacacctga tacccccgag cgtaattcct tgattgacga
    27721 agggatgcaa ggcaccaaac tgattctcgc aggcttcgtc ctcacagccg attgtctccc
    27781 tgtgcccaat gcctggcttg atttttggca ggccaatgca aatggagaat acgataacga
    27841 aggctacacc ctgcgcggac atcaattcac cgacaacaaa ggccgctact accttgaaac
    27901 cattctgcct ggtgaatacc cacaacgccc cattgaacat atccacgtga aagtgcaact
    27961 ccctgctgga gaagtgttca ccacccagtt atatttcccc acccaacccg tcaacggcct
    28021 taccgtgcag ttggaagata aaggcgatca ttttattgcc tacttcaatt ttgtgatgaa
    28081 ataacagaag catgattcac aacaaaaaag agcggcaatt ttcttgccgc tcttttttgt
    28141 tgtctttatg gaatatacac ttcgcaagtc cctgcgctga tgtcacaacg ggccacttct
    28201 tcgccaaccc acaccacacg gaattgagac gagccctctt ttggagtcca gaagaacaac
    28261 tcggcatcca aatccatacg cggttgaatg atgctgacat agccgccgtg gcaatcgggc
    28321 gggtcaatgt agggcgggtc ttcgtctttg tagatcttga tgctcacaca atactttgaa
    28381 gggatatagt tgaatttgag attctcccaa agatgaccag cgaatctgtc atttggtttt
    28441 ccgttttcat cgaggcgttc aaaggaaaag cccgataaac tgcgcggctg acccgatcgg
    28501 ttaagcatgt gaaaactggt ctcattccag aacagggtga ggttgttacc gtcacggtat
    28561 ttgacggttg gaatggctgt gggtgctgcc tcctgtgcca gggttgggat gggggaggcc
    28621 tccaccgtcg gcggcgggac gggagtggcc gaggcggaga tcaaggtttc agttggcggg
    28681 gcaggttcaa cggtcgaatt aagaggcgcg ggagaaaatg ctgcagtggg ggagtctgtt
    28741 tgtgatgctg gtctattctt gaacaggtac cagcctccga tccccaaaaa gaggagaagc
    28801 gccagcacaa ccagccagcc gttgatcttt ttcctgttct gatttgccgc acggacagta
    28861 gcgggcaatt ccacgggctg atggatggtg ttgcctttca ccttgccaga gttgcgcttg
    28921 gtgatgcggt ccatcggaat gccgctgcgg cggacagtgg gtacgccaac tggcagcggc
    28981 ggcaacggaa ttttcggcga tgaatttgcc gcctgaaatg cttcatcaag cgccgacatt
    29041 aattgcgcgc cagtctggta acggttctta gggtttttat cgagcgcctt caacaccacg
    29101 gcttccacgg ctgggctgat ctcagggttg atgcttcgca gtgaaggcgg cggctgtgag
    29161 atgtgctgca gggcaatgct ggctggtgat ggatcgttga acggcactgc gcctgtgaga
    29221 atttcataca ggatgacgcc aagggaatac aggtccgatt gaggcaccgc atctgccgac
    29281 cgacgggcct gctcgggcga aatgtaatgc ggcgtaccga agatattccc catggagcca
    29341 tcgctgactt ccatggccat gccaaaatcg ccgaccaaaa cgcggccatc gtttgcgatc
    29401 aacacgttgg agggttttac atcacggtgg atcactccct gactgtgggc ataatccagt
    29461 gcgcctgcca cagcgttgcc gatgcgcagc acatcggcga tgggcatcaa ctcaccttcc
    29521 tcggcgtaca tttccatgac ggtcgagagg tcgtttccgt ccacatactc catgacgtaa
    29581 taggagtagc ctttttcttc gtcggcgtaa tagatctgga tcaggttttg gtggcgccat
    29641 ttcgccatca tgcgcgcttc cttgatgaag cggctggtat aggcggggtc gttcttgtag
    29701 cgcttatcga tcaccttgat ggcaacaggc cgctgaagtt tcacgtcctg cccaaggtac
    29761 acggtggcca tgccgccctg cccgatcaaa cgttccacgc ggaagttggc taattgctgg
    29821 ccgatcattt gatctgtcat cgattctctg gatttcgctt tcttgttttg gatgtgggca
    29881 gcgtttctct gctggcttgg gtaattctcg aaaggtcacc gatgaatttg ttgaatccgt
    29941 cggcataggc atcaagataa tcctgagtga ttaaattcgc aaggtctttg cgcccgatcg
    30001 agaacagggt tgccacttgc gggcggaaat cgcgcaggga gatgtgcatc acagatttgg
    30061 catcggtgag gacgccccag gcaagctgct tttgttcgtt gtcatctttg gcggtgctga
    30121 aacgggtaag ggtgctcatc ggcaccaccg cgccgacgtt tgaaaatact tcgcggcctt
    30181 tcaataaatt gttcagcaat tcgaagcgtt cgggaatctt gttcaagatc cgctggatgg
    30241 gggaggggag atgggccaac aacttaatgg cgcccacgct ggcgctttcg ccttgaatgg
    30301 caatttcctt tgcccgattc aaatacaggc caaacccagc cagagaagcc agatacattc
    30361 tttgcatctg gtcaaagctt gagtagatgg cggcgcggtc ttcctttgaa tcttcgtagg
    30421 cggcaagcag cttcatggtt tgggcgtgca agctaagcag attcaattcg tggagcggca
    30481 cttccatatt gagaggataa atccgttcac gcgggacctt gagactggca tccatcggaa
    30541 tcaggatcgc agggttggtg cgactccctt cctcaaccac ctggcggatg gctgcggcga
    30601 catcgggttt ggtggcgccg agttttgtga tctcctccag caaatgcgcc gacggtttgt
    30661 acgtggcggc atggatggcg cggtacaaaa ccagcaggtc gttgatggtt agttggatct
    30721 ggtcgttgcg ctgtttgaat tttttccgca gcagcagaca ttccttgagc ttgaccctgt
    30781 cggtctctgc gcctgactct ggcgaaacgt gcggcgcttg ttgcacaagg tctacatcgg
    30841 cgggttggag ctgaagtgtg agcgccgtgt aaacgcgggt atcggctgga gtcggggagg
    30901 ggagcatgct caaataccgc gcccatgaaa gcgcttcgtt cgtcatgatc tctgaaaggg
    30961 ctgtgcccca ggcgccatca aagaaaatat gtgactggtc aaagaccatg gtttcgcccg
    31021 tatcgaaaat ggtcagtgcg tgtgagccga ttccgcgttc ggtttggcgc agctctgaga
    31081 gcggcaatgc aggagaacgc atgtcggtgg agatgaggat gggagcgaac ttcaggttat
    31141 cgagttcgtt taccagcatg gggttgagtt tgccgcgtaa ctccgtcagc gccgaccgtt
    31201 tgatcctcgt cagcgaagca atttgagagg gggaagagaa cggcgacctg agcaaattcg
    31261 cgatctgtgc gcgggcggtc atcacattca atggcttgtt cgagccttgt tcacaaactg
    31321 gaaagagata ataactatcg cgatggataa taccgatctg ggcgtctgtc aggtcaaagg
    31381 ggatacgcga ttcgccgtat gacccaggct ccaaataagc aaggatggac atctggcgct
    31441 gataggcagg caggctgttc tgggtgagta aattcaaccg tgcttcccat ttttcgtagg
    31501 tttgcttcgg cagggaggtt aggttggctt tcaaaaattg gtcaaatttg aagcggcgat
    31561 aggcggggta atcgaggtcg ggggcgtctt cttcgcggcg atagggcagg tgaagctgcc
    31621 agtcacgatg gagacgtttt gcgcgttcac tgctcgattc ttccagcgtg gagatcgaga
    31681 cgcgctcacg ccattcgttt tcgagcagac ggtcatattg atgcaggcag gtgaccgaag
    31741 ccatcagcca tgcagtcaat cggtcaacag ggctcagttg aattttgtgc gcacgaagca
    31801 gggagtcgaa cccgtgggtt tcgttggtgt ggcgggctgt atcttcacgc agggcgtaac
    31861 ttgcatagaa ctgccagacg ccttctggaa aggcggtttc gggatctttg ccagccaatt
    31921 tgagcaggtg ctgatatccc caaatgatgg cggcagggcc agcgaagatc gccccgtaca
    31981 ttgccgaatc tagaatttgt tcaacgccac taaacagggc gttgatatcc tgatgcattt
    32041 tctgcacagg gatgcggcgc ccgggcaggc gggccaatcc gctcaggatc atgccaggca
    32101 cattgccggc aggaagaacc ttggaaacga gatctacaac cgcttccact tctggcaggg
    32161 agaggcgtga aagctgctgg acggagatcg tttttgcaac cgtccgcagg ttggttgcgg
    32221 tcatttgaga tgcttgctgt agatctgatt tttttccact catggctggt gcgtaattat
    32281 aattgcactt gtccatgctc gtattttttc tttcaaaagt gtaaaatcaa cttatgacaa
    32341 tctctgtttt tcaacttacc aaaaggggac cacacaccat tgccacccat gcgcggactc
    32401 tcgatgagat cacccgcgaa ttgccgcagg ggttctacac tacattttcc accctcaacg
    32461 cagggacaga ggtccttggt ttgcacatgc atttgcagag gttgtacgtc cctgcaaagg
    32521 agctgagctt ttcgcctgcc gtggatgagc taaccttgcg acgggaaatc gccgagctgg
    32581 cccgatcctt tttgccaaaa gaggcgcgga tccgtttgat tctggcgaag aacgatgctt
    32641 ccatgtatat cgtccttcaa cagtttgatg cgcttccgaa gaaagtttat gaagaaggag
    32701 tgcaggtggt gacagcgtcg ctggcacgca tggccccgcg agtgaaggac acgggtttta
    32761 tttcatcgag caatcaccag cgcaggctgg tgggcggaga catatttgag gtgttgctca
    32821 caaaggaagg aaaaattctg gaggggatga cttcgaactt ctatgccgtc aaaaagggga
    32881 cgttgatcac atcccagcgc ggaatcctgc ttggggtcac gcgccgcgcc gtgctgcgcc
    32941 tcgcaagagg ggaggggatg tctgtcgaat atcgtccccc gcaggtggat gaaaacttca
    33001 acgaagcttt tctcacgtcc agttcgcgcg gcgtggtgcc gatcgtttcc attgacggaa
    33061 agccggtagg gcaggggagt gtgggggaat gggtgaagcg gttgatgaag gcgtacgcgg
    33121 aatatgtgga gcgaaaggct gagaggattg atggctggta aggttggaac ttacgcaggg
    33181 ggcaattcaa tcatcatgct tccatttcga gtctttttca aaaatatcgt aatatgcctt
    33241 atccaactcc tcgtcagcca gttgggtata tctgtttgtg acttgaatat tctcatgccg
    33301 tgcaaggcgt tgggcaatgg caagattgcc tgtagctctt agggttgtgg tcacaaaata
    33361 atgacggaac gtgtgtggcg tgatttcgcc aaccaggtct tgacctaaaa aacgctccac
    33421 acattcattg acgatgtttc tgccagtggt cgtggtgatc ggtttgatct tttttcctgc
    33481 gcctttgtcg tgccgcgcaa agagggggag ggaacttagt tgtttaccgg aagctccgtc
    33541 cagttcagcg cgttgcgcta aatattcttt cacgacttgc aaagcatatg tggtgaatcg
    33601 aatcacagct tgtttgttgc ctttccctgt aatgatggct ctgccttcgt tccaatcgat
    33661 atcgccgcgc cttagattgc aggcttcatg aacacggagc ccagtttcag ccagcatgaa
    33721 caaaaaagcg cggtcgcgat atgcgcgtag cttagcattt ttttcgcctt cttctgcacc
    33781 taaattgacc agattttgaa catttcgtgc ttgctttaga attttgtcaa tctcgtcaat
    33841 cttatatggc ggagcatgtt tacccggacg ccttgcacgc tgtcgcataa acactgtgag
    33901 ccgcgattga ttgacctcgg tcaatttttc agaatccaga aaaaggtaga agcccttgac
    33961 ggcctgcaaa tacaattgct ctgtggcagt agtgtaggga ctgagatagt ctataaattt
    34021 tgagaactgt tcttcgttga gttgtgtaat gggggtggtg tttggatttg tagatttttc
    34081 attcagcatt tgacagaaaa cattgagcgc gttcttatac gcaagcattg tcttcctgct
    34141 gcgagcgcgt tccaccatgc gcaaatattg ctgaattgct tctgaaatgc tgacgttttt
    34201 ttgttcaagg tctgttgaca tggcctcatt atatgggttt tcctcaggct gtcaatgctt
    34261 tcgctaataa tacttatcga aagcataaga ggtgaaaacg aaacgatgga aataatttca
    34321 cccgccctgc tcccccacgc ggatttttgc gcaggccaga tcacagccca aatttcaccg
    34381 acgcagggaa tcgggcgacg agcttttatt catccttttg ccttcatcgt tcatccttgt
    34441 gtccctcccc tgccccacag atgcggacgt ggaattcttg caaaatgacg catcccccgc
    34501 atttctgccg ggggatgcaa tatgggttac tttcaaattt ggtcgggaaa agagttactt
    34561 cttgggcgga gcggtctttt ccttcttctt ctttcgcttt tccttgttct tgccttgttc
    34621 cttgtttgcc atggatggct cctttcgtga aatgattgga tcctgtatga aaacgccgca
    34681 aacgtgaccg tcagaacaaa ttatacaccc gcgagattgt atcgtccgtg ccaatttgct
    34741 acaatgaatg caaccaattg gatgaaacac aaaaaaggag aacaccatgc cccctgccgg
    34801 actcaaactg ctttacccgg tgaaacccac cttccccatc acccagcgct tcggtgaaaa
    34861 cccgcagtgg tacccgatca cgaacggtca caacggcatt gattggggca ttcccctgcg
    34921 cacccccatc tatgccaccc tgccgggcag cgtggtccgc gtggcggctg ataacagcgg
    34981 ttacgggaat cacatccgca tcagccatga taacaacctg ctttcgctct acggccacat
    35041 ggatgaaagg aattacgctc tcgtcaaggt tggcgatacg gtcacagcgg gccagcacat
    35101 tggctactcc gggaatacgg gccgcagcac ggggccgcac ctgcattttg aagtccgcga
    35161 agggagcacg gccttcaacc ctgagccgta tctcagcgat aacatcaata ttccgcccgc
    35221 aaggacgctt ttcaaagccc gtgtgttgga gaccacgcag cccttcctca acattcggtc
    35281 gggcccgggc atcctgtacc cggtcatcgc caggtacagc cccggcgacg aaatggaagt
    35341 gaccgacatc ggcggcgata cggtctggct gcaaaccaaa aagggatttg tagcctaccg
    35401 cattgacaaa aagaccctca tcgagatcct cggcatggaa gatgaaggct gaagcataaa
    35461 atttgtccgc cacacaaaag cccaccccga aatgaatgtt tcggggtggg ctttttcatt
    35521 aggccgcggt ctgccgtcag cggtcatttc cctttcaaaa accgcgcgat ggatcgtgcc
    35581 acctgttggc cttcaatggc gcaatggtcg gcggtttcca cgccgcgcag taaattccct
    35641 gccgcgaaga ctccatgcac cgacgagcgg aaatccgcat cgatctgtgg acccttcgtc
    35701 tgtgggttga gaatcaggcc gccggtgcga gccagctcat gttcggggat ccagcttccg
    35761 gtgaagatga ccgtatcaca ttccacggtt tcagtttgtc cgcttgcgtg ggtcaactcc
    35821 acggcttcca cccgcctgcg gccgaagatg ttcgatatcc gcgcgttggg tgtgatcggg
    35881 gtgcgggtga ggatatcggc aagcgcccac ttcatcacga tgtatggaaa ttcgatctgg
    35941 tgccgcgctt cttccgtgac catcctttcg catttcaccc cggcatgcat caaggtcatc
    36001 aacgccgaaa gactcaccaa ttccgcaccg acgatgacgg cccgcttccc cactggcagc
    36061 cgctcctgat atacaaagcg ctgtaacgac cccgtggtaa acacaccctg cggacgtgtg
    36121 cccggtatca accgcgccgc ccgcggacgt tcgcgcacgc cggtggccag cagcactgct
    36181 tttgcatcca cctcccccac cccactgggc gaagtgaaag acaactttgc agggccagcc
    36241 catcccaaaa tcgtcgtgga ggtttgtact tccacatcca ttttttcggc gaggtcgcgg
    36301 taatactttg cgtatttcgg tccgctccac atgcgccaca ggtcttcacg gccaaacccc
    36361 gtgtgctgac aaaaacgcgg catgccgcca gcctcaggtt cgcgatccac aacgaggatg
    36421 ttctggatgc cttgcttctt caactcaacc gccgccgaaa gtcccgcagg cccgccgccg
    36481 acgatcaaaa catctttttg cacaacgggc acagggtgtg ccgtgtcttt ttcaaaagtg
    36541 atttgagaag gcacgggcgc aggttctgcg gctgccagcc ttgtcagcgc ggcatggcaa
    36601 ttgaatc
//
LOCUS       DAOQJC010000026        36446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_493_length_36446_cov_9.188810, whole genome shotgun sequence.
ACCESSION   DAOQJC010000026 DAOQJC010000000
VERSION     DAOQJC010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 36446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36446
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_493_length_36446_cov_9.188810"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /gene="uvrC"
                     /locus_tag="PLE39_05640"
     CDS             <1..1142
                     /gene="uvrC"
                     /locus_tag="PLE39_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNE67841.1"
                     /translation="IFATDYKDSDVLAMARSDGEACVQIFFIRGGKLIGREYFILEGT
                     EDTADTEVMAEFVKQFYTEAATIPDQVMIPEEIQVEEAKIIGQWLRSKRGGSKVELFV
                     PKEGQPHELVQMAAENASETLQALRAQWQADAHKQEQALSELQNALKLSAPPNRIECY
                     DVSHTQGVATVGAMIVFEQGVPAKNLYRKFNIDSTSIGAPDDFASMEEMLTRRFKRWL
                     SSQETEAGPGSKKDASFSFLPDLIIIDGGKGQLGRVVRVLEEFELYDKVPVVGLAKQE
                     EEIFFPHNSKPLLLPRHSQGLYLVQRIRDEAHRYGITAHRARRSKQGLASQLDSIPGI
                     GPARRKALLKHFGSLDKIKEASVTDLAAVKGMNETAAESIKAHLE"
     gene            1139..1519
                     /locus_tag="PLE39_05645"
     CDS             1139..1519
                     /locus_tag="PLE39_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE67842.1"
                     /translation="MKQVWIVDDDEEMGHAISLMLKLLDCETKHFINPRPAAQALLAG
                     GRPDLFVLDINMPEVSGLDMLEFLRRRKEWKDLPVIMLSSEAADVMVDKALEMGADGY
                     VMKPVTIDELERVMAQAFYRHITK"
     gene            1816..2892
                     /gene="prfA"
                     /locus_tag="PLE39_05650"
     CDS             1816..2892
                     /gene="prfA"
                     /locus_tag="PLE39_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNE67843.1"
                     /translation="MLDKLQVIEERYEQLGSELLEVGSDYQRAGEINKERVELEPLYN
                     KIREYRQALKSLEEAKSIIISENDSELIALAKADVDDLEPKIEALEKEIKGMLVPKDP
                     RDERNVIFEIRAGAGGDEAAIFAADLFRMYTRYVDGKRWKVELMSESAIGVGGYKEVI
                     FEVKGKGAYSKLKYESGVHRVQRVPATESQGRIHTSTATVTVLAEVDDVEVDIPESDI
                     EIEVYRSSGAGGQNVQKNSTAVRLYHKPTGMIVTCQDERSQLQNKLRALSILRARLYE
                     IEEQKRRAELEADRRSQVGSGDRSEKIRTYNYPQSRVTDHRVGFSSYNLSAVMDGAID
                     QFIDELSTRDESERLAATGVDDDE"
     gene            2941..3789
                     /gene="prmC"
                     /locus_tag="PLE39_05655"
     CDS             2941..3789
                     /gene="prmC"
                     /locus_tag="PLE39_05655"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNE67844.1"
                     /translation="MNAGEYLNNHKASLDGLDAQILLAHVIKRPRTWLLAHLETPLTP
                     PQLVEAEQAFARLIAGEPLPYIIGHWEFFGLDFDITHDVLIPRPETELLVEKALAWLN
                     RHPEARSVADIGTGSGAIAVSIAANIPDAHILATDLSPAALNVAKGNAEKFHVQNQIQ
                     FLECDLLPASSPNLQSSSFNLLCANLPYIPTSTLRQLPIFGREPTLALDGGPDGLDVY
                     RRLFKAAPAWLKPGGMMLFEIESTLGVQAAGLAFDSFSNAVIHLHQDLAGKDRLLEII
                     PSPDAN"
     gene            4066..5127
                     /locus_tag="PLE39_05660"
     CDS             4066..5127
                     /locus_tag="PLE39_05660"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNE67845.1"
                     /translation="MMVVILVHVTISNLAYLIIAKEGMYFNYIIQDVIVLSVLFLFWH
                     LNRRGYTKLAAHACINISIFGAVLLYEPHDLEFTMVILALPIITSSFVLHPIASFFYA
                     LLTTLGYTYSSYYYDSLISYNFTGVLALFAIAFFTFFTAWQLNHTLNKNQFLITDLRS
                     SYETTLDSWSRALDLRDKETEGHTQRVTKLTYRIAKAMRLTEEELLHIRRGSLLHDIG
                     KLGVPDEILHKNGPLTDLELKVMRQHPQFAYDLLYPIEYLRPAMDIPYSHHERWDGSG
                     YPRGLKGLEIPLSARIFAVADVYDALTSDRPYRKAWPPEKVLEYIKNESGAHFDPNIV
                     EIFLHEMKNLNDNKDHLNQ"
     gene            5099..5716
                     /locus_tag="PLE39_05665"
     CDS             5099..5716
                     /locus_tag="PLE39_05665"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNE67846.1"
                     /translation="MTTKIISTSKSHSTEDALLTLQQGGLVAFPTDTVYGLGALAFDN
                     AAIENIYIAKNRPIEKAIPILIGNMDMLHLVTDHIPDMAYTLMARFWPGPLTCVLPKK
                     QTLPPAVSATSTVAVRIPNHPDALTLLTAAGPMAVTSANMSGHPSPTTAQEVYTQLHD
                     RIPLILNGGQTPGGIPSTLVDCTGETPVILREGPITLDELLNALR"
     gene            complement(6007..7032)
                     /gene="tsaD"
                     /locus_tag="PLE39_05670"
     CDS             complement(6007..7032)
                     /gene="tsaD"
                     /locus_tag="PLE39_05670"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNE67847.1"
                     /translation="MDLKPARILALETSCDETACAIVENGRALLSSVVASQMEIHARY
                     GGVYPEVASRQHVLSVTPVVEQALAQAHLTLKDIDALAVTQGPGLAGSLVVGMNMAKG
                     LALGSGLPLIGVNHLEGHLYSAWVHDSGELVPPAPQFPLMALLVSGGHTELNLMTDHL
                     TYQRLGSTLDDAAGEAFDKVSRLLELGYPGGPAIQKSAEEGDPNRFHFPRAWLEGTWD
                     FSFSGLKTAVLYEIRELQKRTHTLPVPDLAASFQKAVVDVLFKKTINAAREFGAKEIL
                     VAGGVSANRMLRQTFRAQTEFPVHIPPLSLCTDNAAMIASAGYHRYALGHLSQMDMDV
                     LPTWPLP"
     gene            complement(7032..8285)
                     /locus_tag="PLE39_05675"
     CDS             complement(7032..8285)
                     /locus_tag="PLE39_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNE67848.1"
                     /translation="MKSELDTLMQAKDMDAILIFGNAEHNPPMYYLTGGGHVSHATLI
                     KKRGGDATLFFGDMERDEAAKSGLKLIPYSTYDFEEIYKKANRNPTLAGAMRLELMLK
                     DVGVESGRVGVYGTYDIGPVFGLLMQLQKLMPNLEFIGEPREDSIFLRAMEKKDEAEV
                     ERIRKMGKVTTTVVGRVADFLTSCEVREDEVLLNENGSALTVGDVHSKIRLWVSELGA
                     ELPSGFIFAIGRDAGVPHSAGNPADLMKLGQTIVFDIYPAEAGGGYYYDFTRTWSLGY
                     ATPEAQELFDQVKEIYDQLNENFDLNVPFKDYQKMTCEYFGGKGHKTPMNSKSPVDGY
                     VHSLGHGVGLNIHERPFSGINTSDEERLAPGVVITSEPGLYYPEKGMGFRIEDTIWVR
                     PDGRMEILADYPYDFVLPMKKWKKK"
     gene            complement(8348..8788)
                     /locus_tag="PLE39_05680"
     CDS             complement(8348..8788)
                     /locus_tag="PLE39_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNE67849.1"
                     /translation="MDEIYIVEKELSYLIMQVAFEVHNTLGPGFPEVIYEEAMNRELA
                     RHGVNLERQKHIKVFYKNEPIGEFVLDNVANGRVILEYKAVAEIAKIHVQQALSYLKA
                     TGLELAIVINFGSTRVQSSRVVFKNGKSNFPSQTPRPRNVLRND"
     gene            complement(8804..10192)
                     /gene="selA"
                     /locus_tag="PLE39_05685"
     CDS             complement(8804..10192)
                     /gene="selA"
                     /locus_tag="PLE39_05685"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HNE67850.1"
                     /translation="MTTLRNLPSIDQILQTQLAAQLIARYGRPLTLSAIRFTLDEIRA
                     RFKSGQITALPLRDLILAQADATLTAWTKPTLIPVINASGVILHTNLGRAPLSDDTIR
                     AMDVASRGYSNLEYDLEKGARGSRLIHAESVLQRLTGAEAAMVVNNNASAVLLVLSAL
                     ANKKRVIISRSQLVEIGGGFRIPDVMKQSGARLVEVGTTNKVHLRDYEEALLGANAIV
                     MRAHPSNFKIIGFTEEPELKDVVALAHAADTILVDDLGSGTFVDTARFGLSHEPTVQE
                     SLAAGADIVCFSGDKLLGGPQAGIIIGKRDLIEKIRKHPLARAVRADKTCLAGITATL
                     LHYLKDEAEREIPIWKMISLTPKQVKGRAEAWAANLGQGTVVEGESTVGGGSLPTESF
                     PTFLLSLSVSSADKFLKKLREQNPPIIARTADNKVLLDPRTVLPEQEGALLVGLKNVM
                     KNLNANAANKRM"
     gene            complement(10189..10587)
                     /locus_tag="PLE39_05690"
     CDS             complement(10189..10587)
                     /locus_tag="PLE39_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67851.1"
                     /translation="MDSQITFRQYLFSTLALLLIGWGGLALLLIYSVPFVWARWGFYV
                     CGIMALTGTALPVVYFLHRRFPTTPQAEPGVIVRQAMWVGVYGATLAWLQLGRLVTLY
                     VILGLAGGLIAIEYFIRLREKANRRLPDIQ"
     gene            complement(10752..12908)
                     /locus_tag="PLE39_05695"
     CDS             complement(10752..12908)
                     /locus_tag="PLE39_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNE67852.1"
                     /translation="MTLPTVSETTLLKEFAYTLRPVERGYANRTLYINLNDNTILEKP
                     VTQQMKDLFTGGRGFALKLLWDAVTPETKWDDPQNELVIANGPICGITAYPGSGKSTV
                     VTLSPLTENVIDSNVGGYFAPFLKFSGFDALEIQGKAAKDVIIFIDGDTGRVTIEADS
                     LEAVDSHQIGNVLTERFGGDEKGKRAISVLSAGQAAEHTRIAMINSTWYDVRRKEARF
                     KQAGRGGPGRVFRDKKIKAIVVRYSDMGGDKNGVADMPLIRKAGQRINKEIADLDDKQ
                     NQMRKVGTANIVEVMDHFDLLPTHNFRYGSHPDTHKIDSKVWKEKFTQGLPDGCWLGC
                     TMSCSHGVDHFPLQTGPYAGDCVLVDGPEYENAAGLGSNIGNFDPQKVLELNFYCDTY
                     GIDSISFANCVAFAMECYEEGIITKEQTGGLELKFGNGSAALDLIHQMARGEGFGVIV
                     GQGVRYMKELFIRQYGADPKFIHDIGMEIKGMEISEYLTKESLAQQGGYGMATKGPQH
                     DEAWLIFMDQVQNLLPSFKDKAEALHYFPMWRTWFSLHGLCKLPWNDISPENNKQTKE
                     PAKVEEHVENYTWIHQGVTGKPTVAEDLLHQSERVYNFQKVFALRMGRVGRKHDYPPY
                     RAMGPVTAAEYESRQDRYDEQLKRLVEVDPANLTTQEKMAHLRKYREEQYEQLMDAVY
                     QRRGWDANSIPTLEKLHQLGIDLPEVVEVVKQAQKK"
     gene            13065..13577
                     /locus_tag="PLE39_05700"
     CDS             13065..13577
                     /locus_tag="PLE39_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNE67853.1"
                     /translation="MNVSEAIRLKRAVRKFQDKPLPDDVIHAILNAGRRSQSSKNNQA
                     WRFIAVREKETLKALSQCGEWAGHIAGAALCVAILTPEPTEKFQTMFDAGQAAAFMQL
                     AAWELGIGSCPASLYDFERTRQILTFPPEWHLRIALSFGYALDEEKISAAPKKGGRVS
                     LDEIVHWEKW"
     gene            complement(13642..16374)
                     /locus_tag="PLE39_05705"
     CDS             complement(13642..16374)
                     /locus_tag="PLE39_05705"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNE67854.1"
                     /translation="MKYKTSRFVHLFFVLILFFNLAGAPAPEMVQPAVTTSNPILFVT
                     QLPIRQDFTTITAVFGNHKAGMQDVGRGGDLWIRYGDGSLKNLTQAAGYGSTGANGFQ
                     DHNAISVRDPYVYWDGTKALFSMVIGAPEQQYTVETYYWQIYEITGLGKNQSPVITKI
                     PNQPEGFNNITPIYGTDDRIIFTSDRPRDGSLHLYPQLDEYEEAPTVSGLWSLDPATG
                     DLFMMNHAPSGDFTPIIDSYGRVIFTQWDHLQRDQQADADKYDGGTYGTFNWSDESAG
                     STPLNIRTEIFPEPRADEEAAGTNLMQHTFNMFMPWQINEDGTEPETINHIGRHELAH
                     YISNSFTDDPNVVYNTPGPIFNPNKADIFLQIKESPTKPGTYFGATMPEFGTHGSGQI
                     ISMTAPPGLDADHISITHITHPDTSSTTATANNSGHYRDPLPLSDGQIIAAHTTQTGG
                     DASSGASIYDFRLKTLAVGGNGYYTASQPLTSGISETISYWSPDTKVTFSGVLWELQP
                     VEVRARTRPAKLTATLEATEQAVFSQAGVNVADFQAYMQANNLALIVSHNVTTRDHSD
                     VQQPLNLRVPGGAETIFASGKVYDVQYLQIFQADQLRGIGGTQSPRPGRRVLAQNLHD
                     PAVAFVQPPNPDGPVGSVVLGLDGSMAAFVPARRALTWQLTDPSGVGVVRERVWVTLQ
                     PGEIRVCKSCHGVNTADQAGNPPPDNEPQALLNLLEYWKVAANSLFADVPASHWAASF
                     IERLYNAGITGGCGTAPLLYCPEATVTRAQMAIFILRGMHGSAYVPPTATGTVFGDVP
                     AGSFAADWIEQLAAEGVTAGCGNGNYCPDATITRAQMAIFLLRGEHGSAYTPPVGTGA
                     VFGDVPAGSFAVDWIEQLAAEGITSGCGGGNYCPDANVTRAEMAVFLVRAFNLP"
     gene            complement(16458..17984)
                     /locus_tag="PLE39_05710"
     CDS             complement(16458..17984)
                     /locus_tag="PLE39_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNE67855.1"
                     /translation="MKNISFFRSICLRVFLSIIFLTSINAVRMNDGPQSPAQVDEAAA
                     TIGTCPTFPQDNFWNTPVTGLPVHPMSDAWVSAIGTNTGFHMDFGSGTWDGGPIGIPY
                     NIVSGSATTKYTVDFYYPDESDPGPYPIPNSPNREWGSDHHILIVDTDDCTLYELYDA
                     SQTTNGWAAGSGAIWDLNSNALRPDGWTSADAAGLPILPGLARYEEVATGIIEHALRF
                     TTNCTADYYIWPARHVAQSGSCANPPPFGARFRLKADYDISGFSPQAQVILQAMKTYG
                     IVLADNGSPWYVSGAPNEGWDNDVLHEMDVLTGASFEAVDTSGMMTTTTFADVPESHW
                     AYQYIEQLYVAGITGGCSTNPLMYCPDSTVTRAQMAIFILRGMHGSAYVPPTATGAVF
                     GDVPAGSFAADWIEQLAAEGVTAGCGNGNYCPDATITRAQMAIFLLRGEHGSAYTPPA
                     GTGAVFGDVPAGSFAVDWIEQLAAEGITSGCGGGNYCPNANVTRAEMAVFLVRAFHLP
                     "
     gene            18267..19109
                     /locus_tag="PLE39_05715"
     CDS             18267..19109
                     /locus_tag="PLE39_05715"
                     /inference="COORDINATES: protein motif:HMM:NF018669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67856.1"
                     /translation="MERLRFLHIPKTAGTTFTTCLDYQYSGKGHFVFKGAVQYDVMRF
                     KELPEAEKQKVVLFTGHAPILTGLEEADTAKIITFLRNPISRVKSFIQHISEGKSPEY
                     LKGPFNLDEFLASGTKDLENLQTKMLINKGPFALSTLIDSMSKSEARDLALNNLFNKV
                     EEYGLQEYFDESLVLFASALNWSTPFYTSLNKKDSSRLLEFKQHHLDRIAELNSIDIE
                     VYNTAKARFLQTLESPKFDKIKLEKLRYKNSLASPHKHAWNLWYSFYRLKRRVRRYLG
                     DESS"
     gene            19191..20915
                     /locus_tag="PLE39_05720"
     CDS             19191..20915
                     /locus_tag="PLE39_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67857.1"
                     /translation="MRKNPLFLLLSLFVILSLFLASCGGEEGEYYTEEATNDEYLTEE
                     PFATEAPASESEGGAPAATNLPGEVVYDLGFNPVDNGFSFSNYGDDIPATNLTADEVR
                     RMFGDQVCARLDGDTCTLTPPAEQWMEQINGAMAGGHCEGMAVLSLMMYTDQVSPSNF
                     GGSTASELDINDEALQREIAYWWATQAVAPTNTSIIKGTPVEILEIVQQMDAKGETYT
                     IGIYKEDGSGGHAITPIGVEDKGNGLYAILVYDNNYPGETRELYVDSRDNTWLYEASI
                     NPQVQSELYTGNADTQTLDLTPTSARLNTQQCPFCNGSGVSSFTGGKLTSPSLQGSQI
                     NQIFLDGEGHILITDENGNRLGYVDGKIVNEIPGATYSAYRMEASGNAPEPIYSLPAD
                     LDVTVSIDGSALTEETLTDLIMIGAGFSIGVEGIYLEPGQVDTAYFYPSEETIAYETE
                     ADESPFIVIGVENPDAEADYYFEVQGADIQGGGIITVYLDTKAGDLLINAEKLNNEGF
                     FDFYLTRITDELEEEFAAEEIALQEGAIVYVNYAEWNDANPEGMYFGVDLDGDGTIDD
                     EYVVDDSQ"
     gene            21067..21378
                     /locus_tag="PLE39_05725"
     CDS             21067..21378
                     /locus_tag="PLE39_05725"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE67858.1"
                     /translation="MKFNPVLFAKAISDETRQKIMNICCCSSLSVSEIVEKLDVSQPT
                     VSHHLAILREADLVTVREDGKQTFYSLNQDNVAYCCGQLMVKFAPESVATENLIKTIK
                     A"
     gene            21636..22601
                     /locus_tag="PLE39_05730"
     CDS             21636..22601
                     /locus_tag="PLE39_05730"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNE67859.1"
                     /translation="MDFPSLLQYTFGKVVETFSHNWFLLLLSIIASAALKLYVDEDAV
                     ARFLRRNTKNSVLMSTGVAVATPLCSCGTTAIVLGMMASTIPWAPIVAFLVASPLTSP
                     QELFYSAGLFGWDFAIAFFVASIVLGLAGGAIASFAESRGWLANQARMAAPKFAATSM
                     GIMGGPVPLDLPVIMQPVAKTEKPTVTFQMFIQEMWTVSRRLLPLFLGFTFIGYMLNG
                     MIPAAWITSLFGSGHVYSIPLAATLGLPFYINTEASLPLVRAMIDSGMSQGAALAFLI
                     TGAGTSLGALGGALTIARWRVIGIVVGTLWVGSIILGFAYNLFIM"
     gene            22622..23419
                     /gene="arsM"
                     /locus_tag="PLE39_05735"
     CDS             22622..23419
                     /gene="arsM"
                     /locus_tag="PLE39_05735"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNE67860.1"
                     /translation="MTQSPTPIHETVREHYAERIKSSASCCGPSDCCTPNNILYPEQL
                     LTTVPSDVASTSYGCGDPITLASLKPGQTVLDLGSGAGLDCILAAQKVGESGHVIGVD
                     MTPEMIERATANAKKVHLTNVEFRQGYLEDLPVESNTVDVIISNCVINLSPDKEKVFT
                     EAFRVLAPGGKLAVSDIVTAGVLPEPVRQSFSAWAGCVAGAVQAEEYIAMMNSVGFTD
                     ISITPVFFDKEFVESAIAEMNLDVSNYPREEIYKAIFSAKITAYKPA"
     gene            complement(23511..23738)
                     /locus_tag="PLE39_05740"
     CDS             complement(23511..23738)
                     /locus_tag="PLE39_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67861.1"
                     /translation="MAESIQIGDRVKIYLDSKFGEKEGWYNGTVFKVDPYSAHRSFYW
                     VELTTEAQTLLGIRQISVFNLKNIQKLQPDS"
     gene            complement(23748..24167)
                     /locus_tag="PLE39_05745"
     CDS             complement(23748..24167)
                     /locus_tag="PLE39_05745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HNE67862.1"
                     /translation="MSDSKVKLERKAKKMIKNEYVIWLTTVDAKHVPQPRPVWFIWDE
                     DSFLIFSQPKAHKVQHIRENGSVSLHFNTDEKGDQDVVIYIGMATLDAKTSPAHKVPA
                     YLRKYRKGIKELGMTLEQFSSEYSTAIRVKPTSLRGW"
     gene            complement(24193..24375)
                     /locus_tag="PLE39_05750"
     CDS             complement(24193..24375)
                     /locus_tag="PLE39_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67863.1"
                     /translation="MVKNVVKGLAVLFFLAGGTWVLQGINVLPGSFMTGDPQWAINGG
                     ITMAVSAGVFWFASRK"
     gene            complement(24444..25265)
                     /locus_tag="PLE39_05755"
     CDS             complement(24444..25265)
                     /locus_tag="PLE39_05755"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNE67864.1"
                     /translation="MKILIAADMEGITGVVHWDQVNPKHPEYARFRKLMTADVNAAIR
                     GAFAGGATSVVVTDGHNSGRNILIEELDARAMLNAGSPAPLSMVQGVDDGVDGVMFIG
                     YHGRTGAADAILDHTWSESVSGVWVNDRAFGEVGLNGAVCGHFDVPIIMASGDQTLCA
                     EVREFFGSEVETAQVKKAVGRMAAECLTPAASASLIEEAAKRAVINLRSGRKTKPFKV
                     SKPIKMTLEFAQSEMADKAALMPGAVRTVDRRVEFSAEDMVTVYLAFRVLMTLAG"
     gene            complement(25274..26047)
                     /locus_tag="PLE39_05760"
     CDS             complement(25274..26047)
                     /locus_tag="PLE39_05760"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE67865.1"
                     /translation="MDANHDVRSYNRAAWDNQVRSGKNPWTVPVSPEVIANARKGDWS
                     VLLTQNIPVPKKWFPPMQGLKILGLASGGGQQGPVFAAAGAEVTIFDNSPAQLERDRE
                     VADRDGLNIRTVEGDMRDLSVFADESFDLVFHPVSNLFIHEIRPVWKEAFRVLRKGGT
                     LLAGFMNPLFYLFDWEDMDNGILNVRHKIPFSDIETYGMERLKQEDRPAEFGHSLTDQ
                     LTGQMEAGFHLIDMYEDHHTGLRISEFTPTYIATRALKP"
     gene            26145..27062
                     /locus_tag="PLE39_05765"
     CDS             26145..27062
                     /locus_tag="PLE39_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67866.1"
                     /translation="MRKYLFITLLTLFALSCGTPAAAAGESLTAADLPVETVTQPAVK
                     NESPKSATTSTTMLYVSATMHIESQPDSWPKDVDAFLAFIQQLTDAGVRLSIGADVGW
                     LTGGIRVQEIIQRTSAMGVQWDVHAHKQSDRAKAAYLLAKYGATPTSVISGMQISEFD
                     KARKPSSYKGYTWTPKVVWGGVACIGHRPGCDDTSIVLYRPTSSAKYTVHDPKGNIVR
                     VGNTDHQLATAESLLAQIEAGAYSVPMLSFTIMIEPETLLIVNSDDGLTEILAFVEQV
                     KENPNLRFATIEETAQAWVAAGSVPIQIP"
     gene            complement(27127..28365)
                     /locus_tag="PLE39_05770"
     CDS             complement(27127..28365)
                     /locus_tag="PLE39_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE67867.1"
                     /translation="MLARLKSIYHEYPKAFWVYNIIVFIDRFGGFMLYPFFALYLTKK
                     FEIGMATVGILFGIFSISGLVGSALGGAITDRMGRKGVIIFSLLLSALSALGMGYAPT
                     LGIFVAVSVIVGTLSSIGGPAHEAVVADLLPENKRAEGYGIIRVIFNMAVIIAPATAG
                     LLIAKSYILLFIIDAVISVVAAGIVLFALPETKPKAHPDAKHETIAQTFAGYGKVFKD
                     IPFMGFVFVTILMALVYMNMNTTLGVYLRDSHGLPELNYGYLLSMNALIVVFFQFWVA
                     RKLEKYKPMLMMALGTALYAIGFAMYGFTSTYLLFVVAMIIITIGEMVVSPFQQALVA
                     SFAPEEMRGRYMAISGLSWGIAFAAGPYMAGWVMENINPNWLWVACGVIGGVAMLGYI
                     VLDRIHHSPVSMAVEPAAAD"
     gene            complement(28451..28690)
                     /locus_tag="PLE39_05775"
     CDS             complement(28451..28690)
                     /locus_tag="PLE39_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67868.1"
                     /translation="MNPLLEEYLVEEHQREIEREMNSAQLLGQTVKAGVYHPTLSTRL
                     LRGFGQWMIEKGEDLVKRHEIPAQNRRTADQRYAH"
     gene            complement(28711..29811)
                     /locus_tag="PLE39_05780"
     CDS             complement(28711..29811)
                     /locus_tag="PLE39_05780"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE67869.1"
                     /translation="MSPFELNLEKTRTETSLVGLEPALNAFGSMLLISKAEDEPGIHE
                     WVSKTRGEMSAEEKFRHGLVTIGFYYSILPQKGGISFEAYLDGLEATPPSVFRERLLK
                     AYEMICITEEAKANQGESVNWEETLSSAENYVDFLKYRFGEDLTNVEMETRAYQYVID
                     PAALKQLVTGHIRWFWKNHLQAEWARVRPMLEESARAFNKLDTDDMTRLEQLRFVTGK
                     DAQETKWADTLENADRVTFVPNAHIGPYMHTTRVGNDLYIYFGAHLPEGSDVQIPELD
                     RAEIVSKLSALADETRLQILQMIAENGKMRSNEVMDATRLSQPSVSRYLGQLTASGFL
                     QERRAGGAKEYSLNRERIEKTLKAVSAFLLDR"
     gene            complement(29971..31140)
                     /locus_tag="PLE39_05785"
     CDS             complement(29971..31140)
                     /locus_tag="PLE39_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE67870.1"
                     /translation="MSQESNPNQKTKVCPTCGTRLSENAMRCLVCGTEFNAKPEPKAA
                     KKVEKSVQASRMPVITMSLPAAVGALAVVVIVAAAIVFFSLRASAPPGTFETAETATP
                     SATPTETQPATATLIPTQVPTSTNIPPFDYTVSAGDGTCSQIAFNFNISVQSIIIANN
                     LPSTCPISVGQVLKIPYPTPTVPPAATNTPEAAEATRISCETVPITVQDGDTLSTIAL
                     NYAVPPEAIKEFNGLPTDNVFIGSSLLIPLCKRAATPGPTPTATIPPPYPAPNLLLPA
                     DGAAFTLANDVVTLQWASVGVLRDGEAYQITIEDVTSDQTRRLTDYVTDTKYIVPTSF
                     RPKDNVAHVLRWWVTPVRQAGTDEQGQPIWVASGAVSDKHVFTWVGVAVEGTPNP"
     gene            complement(31167..31454)
                     /locus_tag="PLE39_05790"
     CDS             complement(31167..31454)
                     /locus_tag="PLE39_05790"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HNE67871.1"
                     /translation="MTAYVIVDIEVSDPEGYKEYVKLAPEAVKLYGGKYIARGGYNET
                     LEGDWQARRLVILEFPSVEQAKNWVNSPEYAPARALRHKYARSNMVVVEGV"
     gene            complement(31581..32876)
                     /locus_tag="PLE39_05795"
     CDS             complement(31581..32876)
                     /locus_tag="PLE39_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNE67872.1"
                     /translation="MSIINEIVLILILILINALLAMSEAALVASRKVRLQQQANDGNK
                     SSELALKLLKDPNIFLSTVQIGITLIGVLAGAVGGATISEALALALQKIPYLGEFSES
                     ISLAIVVLTITILTIWLGELVPKRLGLTSPEKIAQVVAGPMLFTSKAFSPLIKLMSKA
                     TNFALSLMGVDPSSEPPVTEEEIHLLIDQGTQAGVFEEAEQDMVEGIFSLGDSRVYSL
                     MTPRTEVVWLDINDSIEDIREKIAECPYSRFPVRQDSLETIVGIVKSRDLLVESLSGK
                     AVDLKSLLKPAHFIPETMFASRALELFKEKNTELLLVVDEFGGLQGLLTINDILEEIV
                     GAMEFEEPQATQRQDGSWLLDGMLEVDEFKEIFDFESLPHEDEYETLSGFVMTSLGRV
                     PQAADHFEWNNFRFEVIDMDGRRVDKVLVTTLPKPQPPQ"
     gene            complement(33066..33218)
                     /locus_tag="PLE39_05800"
     CDS             complement(33066..33218)
                     /locus_tag="PLE39_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67873.1"
                     /translation="MFRISILELAFTCALILLVLVVPIMVARFQARMDGRLKDIEKKL
                     DKKKQQ"
     gene            complement(33221..34279)
                     /gene="selD"
                     /locus_tag="PLE39_05805"
     CDS             complement(33221..34279)
                     /gene="selD"
                     /locus_tag="PLE39_05805"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(34217..34219),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNE67874.1"
                     /translation="MSIPQSGREIRLTTLSACAGUASKLSADALTQVLRPIKNVFDPA
                     AYPDLLVGLASPDDAAVWRLSEDKAIVVTTDFFTPVVDTPYEYGSIAAANSLSDVYAM
                     GGQPFLALNVAALPDHLPIEISSEIMRGGAEKAREAGVVIAGGHTVKDKEPKFGLVVI
                     GFVDPRKMISKGGLRVGDVLVLTKPLGGGVTTTALKQEKAEERHIAEAIEWMSRLNKT
                     AGQLALEFDLRGGTDITGFGFLGHGLEMAEASGVRLSIENSKVPLLSGARGYAEKGTF
                     PGGAFDNKGHVGENVRFDVGVDEPAQMLLFDPQTSGGLLLGVPREKLSAFETRARELG
                     QPVWVVGEVLDGQGITVQ"
     gene            complement(34420..35436)
                     /locus_tag="PLE39_05810"
     CDS             complement(34420..35436)
                     /locus_tag="PLE39_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HNE67875.1"
                     /translation="MNIAGVIQAVGSFAWLVFVGLLVMIGVRSSRNQPSKGFGTGVVA
                     SLVVAILLTTLGAGIVFIQPEERGIVISAFSSKGYREQSLQPGLRWIIPFAEQVTLYP
                     ISRQTYTMSSSPTEGQIVGDDSIRARTNDGQEILIDASVIYSIDPSKIIELHINWQSR
                     YQDELVRPASRNTIRDAVSQYSVEEIVSTKRAEMEEEITRSLTEAFKENDLVLVSFLL
                     RDIHFSEEYAAAVEQKQIAEQQAQQAKLVVEQKKQEAEQARQVAQGQADAAVIAAQGA
                     AEARLIQAQAEAQANQLLAASLTPTLLQYQYILKLAPGVQTIFIPSGNQFILPLPDTT
                     LQTQ"
     gene            35477..36154
                     /locus_tag="PLE39_05815"
     CDS             35477..36154
                     /locus_tag="PLE39_05815"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67876.1"
                     /translation="MHTGTGLITEIYLDGSAQMDCPPNLIPAPGQYLLAHASGSTSPL
                     PVSVYFYDSAPKGFRFAPPLPPTWTIGTQLNLRGPLGHGFSVPANTRKLALIALESSS
                     ARLNGLIPIALKQGAEVVLVCDSAPSGLPEVVEVQPLRSLEEICKWADFVAVDSARET
                     LPQVKKLFEKLNQVSAVREAQVLIQAPLPCGGLAECGVCAVSSHHPHEWKMACKDGPV
                     FEMNFFI"
BASE COUNT         9059 a         9849 c         8875 g         8663 t
ORIGIN      
        1 tcatctttgc caccgattac aaagattcgg atgtgctggc aatggctcgt tcagacggcg
       61 aagcctgtgt gcagatcttc ttcatccgcg gcggaaagct gatcggccgc gagtatttca
      121 tcctcgaagg cactgaagat accgccgata ccgaggtaat ggcggaattc gtcaagcagt
      181 tttacaccga agccgccacc atccccgatc aggtgatgat ccctgaagaa attcaagtgg
      241 aggaggcaaa gatcatcggc caatggctga gatccaagcg cggcggcagc aaagtggaat
      301 tgttcgtgcc gaaggaaggt caaccgcatg agttggtgca aatggccgct gaaaatgcct
      361 ctgaaaccct gcaggcattg cgtgcccaat ggcaggccga tgcgcacaaa caggaacagg
      421 cgctcagcga attgcagaac gcattgaaac tctctgcgcc gccaaaccga attgagtgtt
      481 acgatgtcag ccatacacag ggtgtggcaa ccgtcggtgc gatgatcgtt tttgagcagg
      541 gtgtgcctgc caagaatctg tatcgcaaat tcaacattga cagcaccagc atcggcgcgc
      601 ctgatgattt tgcttcgatg gaggaaatgc taacccgtag attcaaacgg tggctatcca
      661 gccaggaaac agaagcaggc ccggggtcga aaaaagacgc gtcgttctcc ttcctgcctg
      721 atctgatcat catcgatggc ggcaaggggc agttgggccg tgtggtgaga gtcctggaag
      781 agtttgaact atacgataag gtgcccgtgg tggggctggc caaacaggag gaggaaatct
      841 tcttcccgca taacagcaag ccgttgctgt tgccgcgtca ttcgcaggga ctgtaccttg
      901 tgcagcgcat ccgtgatgaa gcgcatcgtt atggcattac ggcccatcgc gcacgaagat
      961 cgaagcaggg actggcctca cagttggatt ccatccccgg cattggccct gcccgtagaa
     1021 aagcactctt gaaacatttc ggttctctgg ataagattaa ggaagcgagt gttaccgact
     1081 tggctgctgt gaaaggcatg aacgaaacag cggcagaaag cattaaggca catctggaat
     1141 gaaacaagta tggatcgtag acgatgacga ggaaatggga cacgcaatca gtttgatgct
     1201 caaactgctg gattgcgaaa ccaaacattt cattaatccc cgccctgccg cccaggcttt
     1261 gctggctggc gggcggcctg atttgttcgt cctcgatatc aacatgcctg aagtgagcgg
     1321 gttggatatg ctcgagtttc tgcgccgccg caaggagtgg aaggacctgc ctgtcatcat
     1381 gctttcctct gaggcggctg atgtgatggt ggataaagcc cttgaaatgg gcgcagacgg
     1441 ctacgtgatg aaacctgtta caattgacga acttgaaaga gtaatggcgc aagcctttta
     1501 cagacatatt acgaagtgag tggaaagatg gctggaaaaa catccaaaac aaaaaaacaa
     1561 gggaaacccg ctgaccaagg gaattcaaac cagagagccg cacagatcct gttcgcggtg
     1621 tttgcggtca ttctgatcct ttcgatggtg ctttcggcag tcgcaaatta ctaggtacgt
     1681 tcacggttgt tttcgtttcg cctgtgcggc acagctttgt gtgccgcctt ttatttcgca
     1741 cattggaaga gaatataaaa cattccaaac ctcgataccc aagaggttta atatacaaaa
     1801 aactggaatc aaatcatgct cgataaacta caagtcattg aagaacgata cgaacaactg
     1861 ggaagtgaac tgctcgaagt aggcagcgac taccagcgcg ctggggagat caacaaagag
     1921 cgtgttgaat tggaaccgct ctacaacaag attcgcgaat atcgccaggc cctgaaaagc
     1981 ctggaagagg ccaagtccat catcatttct gaaaacgatt cggaattgat cgcgcttgcc
     2041 aaggccgatg tggatgatct ggagccaaag atcgaagcgc tcgaaaaaga gatcaaaggc
     2101 atgctggtgc caaaagaccc gcgcgatgaa cgcaatgtca tttttgaaat ccgcgcaggt
     2161 gcgggcggtg acgaggccgc tatttttgcc gctgacctgt tccgcatgta cacccgctat
     2221 gtggatggca aacgctggaa ggtcgaactc atgtctgaga gtgccatcgg cgtcggcggg
     2281 tacaaggaag taatcttcga agtcaagggc aagggtgcgt actcaaaact gaaatatgag
     2341 tcgggggtgc atcgtgtgca gcgcgtgcca gccacagagt cgcagggacg catccacacc
     2401 tccacggcta ccgtcactgt actggccgaa gtagatgatg tggaagtgga cattcccgaa
     2461 agcgatatcg agatcgaagt ctatcgctct tcgggcgcag gcggacaaaa cgtgcaaaag
     2521 aactccactg cggtgcgttt gtatcacaaa cccactggca tgatcgtcac ctgtcaggac
     2581 gaacgctcgc aattgcaaaa caaactccgc gccctcagca ttttacgggc acgcctgtac
     2641 gagatcgaag aacaaaaacg ccgtgccgaa cttgaagccg accgccgttc acaggtgggt
     2701 tcaggcgacc gttcggaaaa gatccgcact tacaactacc cgcaaagccg tgttacggat
     2761 catcgcgttg gcttttctag ctacaatctt tctgcagtga tggatggggc cattgaccaa
     2821 ttcatcgatg agttgtcaac ccgggatgag tccgaacgcc tcgccgccac gggtgtggat
     2881 gatgacgaat aatcgaatgt ggcggctgca tggtcgccac attttctttg ggttcaactc
     2941 atgaatgcgg gagagtatct caacaatcac aaggcttctc tggatgggct tgacgcgcag
     3001 attctccttg cgcatgttat taaacgtccg cgaacatggc tgcttgccca ccttgaaacg
     3061 cctcttaccc cgccccagct tgtcgaagca gaacaagcat ttgcccgcct gatagcgggt
     3121 gaacccctcc cctacataat cggtcattgg gaattcttcg gtcttgattt tgacatcacc
     3181 catgatgtcc tcattccacg ccctgagaca gaacttctgg tagagaaagc tctcgcatgg
     3241 ctgaacaggc accccgaagc acgaagcgtg gctgatatcg gcactggttc aggggcaatt
     3301 gccgtatcca tcgccgcgaa catcccggat gcccatatcc ttgccactga tctttcgccc
     3361 gccgctttga atgtggcaaa aggcaatgcc gaaaaattcc acgttcaaaa tcaaattcag
     3421 ttcctggaat gcgacctgct ccctgcttca tcacccaacc ttcaatcttc atctttcaac
     3481 cttctctgcg ccaacctacc ctacataccc accagcacat tgcggcagct tcccatcttt
     3541 ggccgtgaac ccacccttgc ccttgatggc ggccctgatg ggcttgatgt gtaccgccgc
     3601 cttttcaagg ctgcgcctgc ctggctcaaa ccaggaggca tgatgctttt tgagatcgaa
     3661 tccacattgg gggtgcaggc ggccggtctt gccttcgatt ccttctccaa tgcggttatt
     3721 catctgcatc aggatctggc aggcaaagac cgcctgctgg agatcatccc atctcccgat
     3781 gcaaattaag cgcaggttgc cgcccttcca aaatcatgcc atactcagca ccacgcatcg
     3841 acaaaaatcc gtgcaaaatt gtccaggcgc gaaactgcaa gcaaacacca agatttcaac
     3901 ctgaaaaacc atgtaatact tctccaattg agtaacagac agtctatgaa tcgctacaac
     3961 ggaatagtcc ccaaattaaa tagcctatac tatcaggtca cagatacaga cgcagtggaa
     4021 cccagcgaac agcgacgagg ccgtctgatg gcaatgtacc tgatcatgat ggtagtcatc
     4081 ctggtacatg tcacgatctc caatcttgcc tatttgatca tagctaaaga gggaatgtac
     4141 ttcaactaca ttattcaaga tgtgatcgtt ctgtcggttt tattcctttt ttggcatctc
     4201 aaccgcagag gctacacaaa actggccgcc catgcctgca ttaatatttc catttttggg
     4261 gcggtgctcc tgtacgaacc tcatgacctt gaatttacga tggtcattct ggctctcccc
     4321 atcattactt ccagttttgt cctccacccg attgcatcct ttttttatgc cctgctcacc
     4381 acccttgggt acacctactc cagctattac tacgactccc tgattagcta taatttcacc
     4441 ggggtactcg ccctttttgc catcgcattt ttcaccttct tcactgcctg gcagcttaat
     4501 cacaccctga ataaaaatca attcctcatc accgatctgc gctcttccta tgaaaccaca
     4561 cttgatagct ggtcgcgcgc cctggacctg cgagacaagg aaaccgaagg ccacacgcag
     4621 cgcgtcacca aacttaccta tagaattgcc aaggcaatga ggcttacgga agaggaactg
     4681 ctccacatcc gccgcggatc gctgctgcac gatattggaa aacttggcgt ccccgatgag
     4741 atactgcaca aaaatggtcc cctgacagac cttgaattga aggtcatgcg tcaacacccg
     4801 caattcgcct acgacctgtt ataccccatc gaatacctgc gccccgccat ggatattccc
     4861 tacagccatc acgaacgctg ggatggcagc ggatatccgc gcggattaaa ggggctggag
     4921 attcccctct ctgcccgcat ctttgccgtt gccgatgtat acgatgcgct cacctcagac
     4981 cgcccctacc gcaaagcatg gccgcctgaa aaagtactcg aatacatcaa aaatgagtcg
     5041 ggcgcacact tcgacccaaa tatcgtggaa atctttttgc acgaaatgaa gaacctgaat
     5101 gacaacaaag atcatctcaa ccagtaaatc tcattccacc gaagatgccc tgttaaccct
     5161 tcaacaaggc ggccttgtag ccttccccac cgatacagtc tacggccttg gcgcgctcgc
     5221 ctttgataac gccgccatcg aaaacatcta catcgccaag aaccgtccca tcgaaaaagc
     5281 cattccaatt ttgatcggga acatggatat gctccacctt gtcactgacc atattccaga
     5341 catggcctac accctcatgg cccgcttctg gcccggaccc ctcacctgtg tgctccccaa
     5401 aaagcagact ctccccccgg cagtatctgc cacctccact gtagccgtca gaatcccaaa
     5461 ccacccagat gccctcacct tgttaactgc cgcaggcccc atggcagtta cctctgccaa
     5521 tatgtcagga catcccagcc caaccacagc acaagaagtt tacacacaac tccacgaccg
     5581 cattccgctc attttaaatg gcggccaaac cccaggcgga atcccatcca cactggtgga
     5641 ttgcacaggt gaaacacccg tcattctacg ggaaggtccc attactttgg acgaacttct
     5701 caacgcactc cgttgaccga atctctaatc ctgtgttcat ttcccattca ggctgttttt
     5761 agctcactga atataataaa gttacattct ccttatcgcg ggccccgatt tttcagactt
     5821 gggcaagttt gaaacggggc ctcgatgttt taaatttcag gcacattttc tctcatccaa
     5881 ctcttaatca atgctcaggt tcagtttagg tgacgattct aaaatacagt tacattctcc
     5941 ctaaaccaga aggccgatga cttgggcaag tcatcggcct tctggtttta atgcatgatt
     6001 ttatcgttac ggcaatggcc aggtcggcag aacatccatg tccatctgtg aaaggtgacc
     6061 aagtgcatag cgatggtacc ctgccgaggc gatcatggcg gcattgtcgg tacacaacga
     6121 cagtggcggg atatgaacag gaaattccgt ttgggcccta aaggtctggc gcagcatacg
     6181 attggctgaa acgccaccag ccaccaaaat ttccttcgcg ccaaattcac gagcggcatt
     6241 gatggttttc ttgaaaagaa catccaccac agctttttga aaagatgcag ccagatcggg
     6301 tacgggcagg gtgtgtgtcc gcttttgcaa ttcgcggatc tcgtacaata cggcggtctt
     6361 caacccgctg aacgaaaaat cccatgtccc ttcgagccat gcccgaggaa aatggaaacg
     6421 attcgggtcg ccctcttccg ctgatttttg gatggcaggg ccgccgggat atccaagctc
     6481 caacaggcgg gaaactttgt cgaaggcttc accagctgca tcgtcgaggg ttgaaccgag
     6541 cctttgatag gtcaaatgat cggtcatgag attcagttcc gtatgcccgc ctgaaaccag
     6601 caaagccatc aaggggaatt gtggtgcggg cggtacgagt tctcccgaat catgcaccca
     6661 ggccgaatac aaatgccctt caagatggtt cactccgatc aatggaagcc cggaccccaa
     6721 ggcaagtccc tttgccatgt tcatccccac aaccaacgaa cccgcgaggc caggcccttg
     6781 tgtgacggcc agtgcgtcaa tatctttgag cgtgaggtga gcctgtgcaa gagcctgctc
     6841 tacaacaggc gttacgctca aaacatgctg gcgtgaagcc acctcgggat acaccccgcc
     6901 atagcgcgca tgaatctcca tctgtgaggc aaccacagaa gaaagcaatg cgcggccatt
     6961 ttcaacgatg gcacaggcgg tttcatcgca ggaggtttca agggcaagga ttcgggcggg
     7021 tttcaggtcc attatttttt cttccacttt ttcatcggca aaacaaaatc atacggataa
     7081 tctgcaagga tctccatacg cccatcgggg cgcacccata ttgtatcttc gattcggaag
     7141 cccatgccct tttcaggata atacaagcct ggctcagagg tgatcaccac cccgggcgca
     7201 agccgttcct catcgctggt attgatgccg ctgaatggac gttcgtgaat gttgagcccc
     7261 acgccgtgac ccaggctgtg aacataccca tcgacaggcg atttggagtt catgggggtt
     7321 ttatgtccct tgcccccgaa atattcacag gtcatttttt ggtaatcttt aaacgggaca
     7381 ttcaggtcaa agttctcgtt caactggtcg tagatctctt taacctgatc gaacaattcc
     7441 tgtgcctctg gcgtggcgta acccaaactc caggtgcggg tgaaatcgta gtagtagccg
     7501 ccgcccgcct cagcaggata aatatcgaac acgatcgtct gcccaagttt catcaggtcg
     7561 gctgggttgc ctgcggaatg cggcacgcct gcatcgcggc caatggcaaa gatgaatcca
     7621 gaaggtaatt ctgcgccgag ttcagatacc cataaacgaa tcttcgaatg gacatcgccc
     7681 acggtcagag cggatccatt ttcgttcaaa agaacctcat cttcacgaac ttcgcaggac
     7741 gtcagaaaat cagcaacacg gccaaccacg gttgtggtga ccttgcccat tttgcggata
     7801 cgctccactt cggcttcatc tttcttttcc attgcgcgca ggaaaatgga atcttcccgc
     7861 ggttcgccga taaattcgag attcggcatc agcttttgca actgcatcaa taatccaaaa
     7921 acgggaccga tgtcatatgt tccgtacacc cccacccgac ccgattccac acccacatct
     7981 ttgagcatca gttcaaggcg catggcgccc gccagcgtgg ggtttcgatt cgcctttttg
     8041 tagatctctt caaaatcata agtactgtag ggaatcaatt tcaatccgct ttttgcagct
     8101 tcgtcacgtt ccatatcacc gaagaaaagg gtcgcatcgc cgccgcgttt tttgatcaga
     8161 gtggcatggc tgacatgtcc accacctgtc aggtaataca tgggcgggtt gtgttcggca
     8221 ttgccaaaga tgagaatggc atccatgtct ttggcttgca taagggtatc aagttctgat
     8281 ttcataagtt tcctttttat aagagtcttt aacgcgaata gagcgaatgt gcgaataaaa
     8341 cgaattttta atcgttgcgc agaacattac gcggccgtgg cgtctgcgat gggaaattag
     8401 atttaccatt tttgaaaaca acacgactgg attgaacccg tgtcgatcca aaattaatga
     8461 caatcgccaa ttcaagaccg gttgctttca agtatgaaag cgcctgttgc acatgaattt
     8521 tcgcaatctc cgcaacagcc ttgtattcaa gaattacacg tccattagct acattatcaa
     8581 ggacaaactc accaattggt tcgtttttat aaaaaacttt gatatgtttt tgacgttcca
     8641 aattaactcc gtgtcttgcg agctcacgat tcatagcctc ttcataaatt acttctggaa
     8701 aacctggccc gagcgtatta tgtacctcaa aagccacttg cataatcaaa taagacagtt
     8761 ctttctctac tatataaatt tcgtccatcc caaactccat tcgttacatt cgcttattcg
     8821 cagcattcgc gttaaggttt ttcatcacat tcttcaaacc aaccaacaag gccccttcct
     8881 gttcgggcaa aacggtgcgc gggtcaagca aaactttatt atctgccgtg cgtgcaatga
     8941 tcggcgggtt ttgttcgcgc aactttttca ggaatttatc ggcagatgag accgataatg
     9001 acaataaaaa tgttgggaag gattccgtgg gcaggcttcc gccgcctact gtggattcac
     9061 cctccacgac cgttccctgc cccaaattgg cagcccacgc ttccgcccga cctttgacct
     9121 gcttcggcgt aagagagatc atcttccaga tgggaatttc acgctccgct tcatctttga
     9181 ggtaatgcaa aagggtggcg gtgatacccg caaggcaggt cttatccgcg cggacagcac
     9241 gcgccagcgg atgcttgcga atcttctcga tcaaatcccg cttcccgatg atgatccctg
     9301 cctgtggccc gccgagcagt ttatcaccag aaaaacagac aatatctgcc ccagccgcaa
     9361 gggattcttg tacggtgggt tcatggctga ggccaaacct ggcagtgtca acgaatgtgc
     9421 cagaaccgag atcatccacc agaatcgtgt cggcggcatg tgccagcgca acaacatcct
     9481 tcaattcggg ctcttcggta aaaccaatga tcttgaaatt cgatgggtgt gcccgcatga
     9541 caatggcatt cgcacccagc aaggcttctt cataatcccg caaatggact ttgttggtcg
     9601 tgcccacttc caccagcctt gcacctgatt gcttcatcac atcaggaatg cggaagccgc
     9661 cgccgatctc caccaattgc gaacgtgaaa tgatgacccg ctttttattc gccaatgcag
     9721 aaagaaccag caaaaccgcc gaagcgttgt tgttgaccac catcgccgct tcagccccag
     9781 tgagtctctg caaaacagat tcagcatgga tgagccgtga accgcgcgcg cctttttcaa
     9841 gatcgtattc gaggttggaa tagccgcgtg aagccacatc catggcgcgg atggtgtcat
     9901 cgctgagcgg ggcacggccg aggttcgtat gcagaatcac ccccgaagcg ttgatgaccg
     9961 ggatgagggt cggctttgtc catgcggtga gggtggcatc tgcctgagcg aggatcaaat
    10021 ctcgtagagg cagggcagtt atctgtccag atttgaagcg ggctcttatt tcatccaatg
    10081 tgaaacgaat ggcgcttaag gtcaatggcc tgccatagcg ggcaatgagc tgtgcagcca
    10141 actgtgtttg cagaatttgg tcaatggaag gtagattgcg aagcgttgtc attggatatc
    10201 gggcaggcgg cggttggctt tttctcgcag gcgaatgaaa tattcaattg cgatcaggcc
    10261 gcccgccaaa ccgagaatta cataaagtgt gacgagacgc ccaagttgaa gccatgccaa
    10321 agtggcccca tacaccccca cccacatggc ctgccgcaca ataacgcctg gctcggcttg
    10381 tggagtggtc ggaaagcggc ggtgtagaaa atagacaaca ggcaaggccg tgcctgtaag
    10441 cgccataatg ccgcagacgt agaatcccca acgggcccat acgaagggca cagaataaat
    10501 caacagcagg gcaagtccgc cccagccgat gagcaggagg gcaagcgttg aaaaaagata
    10561 ttgtcgaaat gtgatttgcg aatccattgc acgaattata accgcagagg gaaaaggtca
    10621 tttgcgatga tataaaacca tccgctcgaa tttttaacac aaagccgcca gggcgcgggg
    10681 attgcaatgg ctttgcatct ccgcgccctg gcgttaaatt ttctggttct gtaaaacaga
    10741 gatttcgtga tttacttttt ctgcgcctgt ttgacgacct ctacgacttc aggcaaatca
    10801 atgccgagtt gatgaagttt ttcgagggtg ggaatgctgt tcgcgtccca gccccttctt
    10861 tgatagaccg cgtccatcaa ttgttcatat tgttcttcgc ggtatttgcg gaggtgcgcc
    10921 atcttttcct gtgtggtcag gtttgcgggg tcaacttcaa ccagccgttt gagttgttcg
    10981 tcatagcgat cctgacggga ttcgtattca gcagctgtga caggtcccat tgcacggtaa
    11041 ggcgggtaat cgtgtttgcg tcctacacga cccatgcgca aggcaaagac cttttggaag
    11101 ttgtacactc tctccgattg atgcagcagg tcttcggcaa ccgttggctt accagtgacg
    11161 ccctgatgga tccaggtata gttttcaacg tgttcttcca ctttggcggg ttctttggtc
    11221 tgcttgttgt tttcgggtga gatgtcgttc cacggcagtt tgcacaaccc gtgcaggctg
    11281 aaccatgtgc gccacatggg gaagtaatgc aaagcttcgg ctttgtcttt gaaagagggc
    11341 agcaggtttt gcacttgatc catgaagatc agccaggctt catcatgctg tggacctttg
    11401 gtggccatgc cgtagccacc ctgctgagcc agcgattctt tggtgaggta ttcggaaatt
    11461 tccatgccct tgatctccat gccaatatca tggatgaatt tcgggtcagc gccgtattga
    11521 cggataaata gttctttcat atagcgaaca ccctgcccca cgatcacacc aaagccttcg
    11581 ccgcgtgcca tttggtgaat gagatccaaa gcagccgagc cgttgccgaa tttcaattcc
    11641 aaaccgcctg tttgttcctt tgtgataatg ccttcttcat aacattccat cgcaaaggca
    11701 acgcagtttg cgaatgagat gctgtcgatg ccgtaggtat cgcaatagaa attgagttcc
    11761 aaaacctttt gcgggtcgaa attgccgatg ttcgaaccca gcccagcggc attctcatat
    11821 tcagggccat ccaccagaac gcagtcacct gcgtaaggtc cagtttgaag cgggaagtga
    11881 tccacaccat gagaacatga catggtgcag cccagccagc agccgtcggg taatccctgt
    11941 gtgaattttt ccttccaaac tttcgaatcg attttatgcg tatcgggatg tgatccatag
    12001 cggaaattat gtgtgggcag cagatcgaaa tggtccatta cttcgacgat gttggccgtg
    12061 cccacttttc gcatttgatt ctgcttgtca tcgaggtcgg cgatttcctt gttgatacgc
    12121 tgaccagcct tgcggatgag cggcatgtcg gccacaccgt tcttgtctcc gcccatatcg
    12181 gagtagcgta ccacgatggc tttgatcttc ttatcgcgga agacacgccc tggaccgcct
    12241 cggcctgcct gtttgaaacg ggcttccttg cggcgcacat cgtaccaggt tgaattgatc
    12301 atggcgatgc gggtgtgttc cgccgcctga cctgccgaaa gcacggatat ggcgcgtttg
    12361 cctttttcgt caccgccaaa tctttcggtg aggacattgc cgatctggtg tgagtcgaca
    12421 gcttcgagtg agtctgcttc gatggtcact cgtccagtat cgccgtcaat gaaaatgatc
    12481 acatctttgg ctgccttgcc ttgaatctcg agcgcgtcaa aaccagagaa tttcaggaag
    12541 ggggcaaaat atccgcccac gttgctgtcg atcacatttt cggtcaacgg gctcagggta
    12601 acaactgtgg atttgccgct gccggggtag gctgtgattc cacagatcgg gccgttggca
    12661 atgaccagtt cattttgcgg gtcatcccat ttcgtttcgg gggttacggc atcccacagt
    12721 aatttgagtg caaatccgcg cccgccggtg aacaggtctt tcatttgttg ggtgacgggc
    12781 ttttccagga tggtattgtc gttcaggttg atgtacagcg tacggtttgc atatccacgc
    12841 tctacggggc gcagggtgta ggcaaattct ttcaataggg tggtttctga aacagtgggt
    12901 aaggtcatag ggggctcctg tttttatata aaaattgaca aattaccagt ttcattatca
    12961 caaaatgagg tcaaactgcc tattgactat cttccctttt tgatagtgcc aagagcaaca
    13021 tccattttca acggtaggct ataatcctcg aaaagcgagg aaccatgaac gtatctgaag
    13081 ccatccgtct aaaacgcgcc gtgagaaaat ttcaagacaa acccctgcca gatgatgtga
    13141 tccacgccat tctcaatgca gggcgcaggt cgcaatcttc gaagaacaat caggcctggc
    13201 gtttcattgc cgttcgtgaa aaagaaacgc taaaggcttt gtcacaatgc ggagagtggg
    13261 caggccatat tgcaggtgcg gccttgtgcg tggccattct cacccccgaa cccaccgaaa
    13321 aattccagac gatgtttgac gcagggcaag ccgccgcctt tatgcagctc gccgcatggg
    13381 aattgggcat tggatcatgc cccgcttctc tctatgactt tgaacggact cgccaaattt
    13441 tgacattccc tcccgaatgg catttacgta tcgccctctc cttcggctat gccctcgacg
    13501 aagagaaaat ctccgccgcc cccaaaaaag gcggacgggt atcgctggat gagatcgtac
    13561 attgggagaa gtggtgatta aacataaaaa tgccgttgac tttttgtcaa cggcattttt
    13621 atttatcagc aattcgattc tttacggcaa gttgaatgct ctcaccaaaa agacagccat
    13681 ctccgcacgg gtcacatttg catcggggca gtaattccca ccgccgcatc cactggtgat
    13741 gccttcggct gcgagttgtt cgatccaatc cacagcgaag gaacctgcgg gcacatcccc
    13801 aaagacagcg cccgttccaa caggcggagt gtacgcactg ccatgttcgc cgcgcaacag
    13861 gaagatcgcc atttgggcgc gagtgatggt ggcatcgggg cagtaattcc cgttgccgca
    13921 accagccgtg accccttctg ccgctagctg ttcgatccaa tccgcggcaa aagaacctgc
    13981 tggtacatca ccaaaaactg tgcccgttgc tgtgggagga acataggcac tgccatgcat
    14041 tccacgcaaa atgaagatcg ccatttgtgc gcgggtcacg gtcgcctcgg ggcagtaaag
    14101 caacggtgcc gtaccgcatc cacctgtaat gcctgcattg tacaaacgct caatgaaact
    14161 ggctgcccag tgtgaagcag gcacatcagc aaaaaggctg ttagccgcca ctttccaata
    14221 ttccagaaga ttcaacaatg cctgcggttc gttgtcagga ggcgggttgc cagcctggtc
    14281 cgcggtattt acgccatgac aggatttaca aacacgaatc tcacccggtt gtaacgtcac
    14341 ccacacacgc tcacgcacca cccccacccc ggaaggatcg gtcaactgcc aggtcaaagc
    14401 ccgccgggca gggacaaaag cggccatgga accatccagt cccagaacca cactccccac
    14461 cgggccatcc gggttgggcg gttgaacgaa ggcaacagcc gggtcatgaa gattttgagc
    14521 aagtactctt cgtcccggcc ttggtgattg tgtgcctcca attccacgca attgatcggc
    14581 ctggaagatc tgcaggtatt gcacgtcata caccttccca gaggcaaaga tcgtttctgc
    14641 accaccaggc acacgtaagt tgagcggctg ttgcacatcc gaatgatcgc gggtggtgac
    14701 gttatgactg acgatcagag ccagattatt cgcctgcata tacgcctgaa aatcagccac
    14761 attgacaccg gcctgagaaa aaaccgcctg ttctgttgct tccagtgttg ctgtcaattt
    14821 cgcagggcgt gtacgtgcgc gtacttccac gggctgaagt tcccacaaca caccggaaaa
    14881 ggtcactttt gtatcggggc tccaataact gatggtttcc gaaatcccag aggtcaatgg
    14941 ctggcttgcg gtgtaatatc cattgccacc cacggcgaga gtttttaagc ggaagtcata
    15001 aatcgacgct ccagaagatg catcgccccc ggtctgagtg gtatgggcgg caatgatctg
    15061 accatccgaa agcggcaacg ggtcgcggta gtgaccggaa ttgttggccg ttgcagtggt
    15121 gctggatgta tctggatggg tgatatgggt aatggaaata tggtcagcat ccaatccggg
    15181 aggggctgtc atactgatga tctgcccgga gccatgcgta ccgaattctg gcatggtcgc
    15241 gccgaaatac gtccctggct ttgtcggcga ttctttaatt tggaggaaaa tatccgcctt
    15301 attggggtta aagatcgggc caggcgtgtt gtaaaccaca ttgggatcat ctgtaaatga
    15361 attggaaatg taatgtgcca gttcatggcg gccaatgtga ttgatggttt cgggttcggt
    15421 gccatcttca ttgatctgcc agggcataaa catattgaat gtgtgctgca tcaggttcgt
    15481 ccctgctgct tcttcatctg cacgcggctc tggaaagatt tcggtgcgaa tgttgagcgg
    15541 cgtggagcct gccgattcat ccgaccagtt gaaggttccg tatgtaccgc catcgtattt
    15601 atccgcatca gcctgttgat cccgttgaag atgatcccac tgcgtaaaga tgacacggcc
    15661 atagctatcg atgatgggag tgaaatcacc ggatggggca tggttcatca tgaacaggtc
    15721 accagtggca gggtcaaggc tccacaaccc gctgacagta ggcgcttcct catattcatc
    15781 caactgcgga tacaaatgca acgaaccatc acgcggacga tcagaggtga aaatgatgcg
    15841 gtcatccgtg ccgtagatcg gtgtaatatt gttgaaccct tcaggctggt ttggaatttt
    15901 ggtgatcaca ggggattgat tctttcccaa cccggtgatt tcatagatct gccagtaata
    15961 ggtttccaca gtatattgct gctccggagc gccgatgacc atgctaaaaa gcgccttcgt
    16021 accatcccaa tacacatacg gatcgcgcac agaaattgcg ttatggtcct gaaatccatt
    16081 ggcacctgtg ctgccatacc cggcagcctg agtcagattc ttaagtgaac catcgccata
    16141 acggatccat agatcgccgc cgcgccccac atcctgcatc cctgctttat ggttgccaaa
    16201 tacggcggtg atggtcgtaa aatcctggcg gatcgggagc tgcgttacaa acaaaatggg
    16261 gttggaggtg gtcactgcag gttgaaccat ctcaggcgca ggcgctcctg caaggttaaa
    16321 aaacagaatt aatacaaaaa aaagatgaac gaaccgcgat gttttatatt tcattattaa
    16381 attctccatg acaatatttt ggaaatcaaa aagtgcctca atacacaaag gcactttttg
    16441 atttttagca aactcgttta cggtaaatga aatgccctca ctaaaaagac agccatttct
    16501 gcacgggtga cattggcatt ggggcagtaa tttccgccgc cgcagccgct ggtgatgcct
    16561 tcggctgcga gttgttcgat ccaatccaca gcaaaggagc ctgcgggtac atccccaaag
    16621 actgcacctg tccctgcagg cggggtgtaa gcactgccat gctcaccgcg caacaggaag
    16681 atcgccattt gggcgcgagt gatggtggca tcggggcagt aattcccgtt tccacaacca
    16741 gccgtgaccc cttctgccgc tagctgttcg atccaatccg cagcgaaaga acctgctggt
    16801 acatcaccaa agacagcgcc cgtcgctgtg ggtgggacat aggcactgcc atgcattcca
    16861 cgcaaaatga aaatcgccat ttgtgcgcgg gttacggttg aatcggggca atacatcaat
    16921 ggattggtcg aacagccccc cgtgatgcct gcgacgtata actgctcaat atattgatat
    16981 gcccaatgcg actcggggac atcggcaaag gtggttgtgg tcatcatgcc agaggtatcc
    17041 acagcctcga agctggcccc ggtcaagacg tccatttcgt gcaggacatc attgtcccaa
    17101 ccttcgttgg gggcaccgct cacataccag ggagagccat tgtctgcgag tacgatgccg
    17161 taggtcttca tcgcctgcaa gatgacctgt gcctgtggtg aaaagccgct gatgtcataa
    17221 tccgctttca ggcggaagcg cgcgccaaag ggcggcgggt tcgcacaact tcctgattgc
    17281 gctacgtgcc gcgcaggcca gatgtaatag tcagcggtgc agttggtggt aaaacgcaaa
    17341 gcatgttcga taatccccgt ggctacttct tcatagcgtg ccagcccggg caagatcggc
    17401 aaaccagccg catcggcgga tgtccagcca tcggggcgca gggcattgga gttcagatcc
    17461 cagatcgcgc cagagccagc ggcccatcca ttggtggttt gagaagcatc ataaagttcg
    17521 tacaatgtac aatcatcagt atccactatc aggatatgat gatccgaacc ccattcacgg
    17581 ttgggactgt ttggaatggg atacggcccg gggtctgatt cgtccgggta gtagaaatcc
    17641 actgtatatt ttgttgtagc ggaacccgaa acaatgttgt agggaatgcc gatcggtccc
    17701 ccatcccatg tgcctgaacc gaaatccata tggaagccgg tgtttgtgcc aatggcgctg
    17761 acccatgcat cagacatggg gtgaacgggc aagcctgtga ccggtgtgtt ccaaaagtta
    17821 tcctgcggga aggttgggca ggttccgatg gtggcggcgg cctcatcaac ctgggcgggg
    17881 ctttgagggc catcgttcat gcggacggca ttgatggatg tcaggaaaat aatgctgaga
    17941 aatactctta aacaaataga gcggaaaaaa ctgatattct tcatttgttt gtttcctttt
    18001 cttccttggg gtggaatttt atgggtactg aaaaaacatg tcacctctct aaacggaggg
    18061 gaaaaaccga caaaatcgaa agcatatggg gcacgatccc agtcactctc tctgcataaa
    18121 ttttataaaa cttatcaggg ataacaacca tgatgtaggt actaaaaaaa atcgtgttat
    18181 tgacagtttt gcaagggcga tactcattgg cttataattg ccatcctgcc cgccttgcag
    18241 gcggcaaatc ttttatggag accttcatgg agcgtttgag attcctgcac attcccaaaa
    18301 ctgcgggaac cacatttacc acctgccttg attatcaata ttcggggaag ggacattttg
    18361 ttttcaaggg tgcggttcaa tacgatgtga tgcgattcaa ggaactccct gaggcggaaa
    18421 aacagaaggt cgttctgttc acaggccatg cgccgatctt gaccgggctg gaagaagctg
    18481 atacggcgaa gatcatcact tttttgagga atcccatcag ccgtgttaaa tcttttattc
    18541 agcatatttc cgagggaaaa tcgcctgagt atttgaaggg gcctttcaac cttgatgagt
    18601 ttctcgcaag cggtacaaag gaccttgaga atttacagac caagatgctc atcaacaagg
    18661 ggccattcgc cctttccacg ttgatcgatt cgatgtctaa atctgaggcg cgcgatctgg
    18721 cgctgaataa tctgttcaac aaggtggaag aatacggttt gcaggagtat tttgacgaaa
    18781 gcctggtgct ttttgcctct gcgctgaatt ggtccacacc attttacaca tcgctgaaca
    18841 aaaaagattc cagccgtttg ctggaattca aacagcatca cctcgaccgc atcgcagaac
    18901 ttaattccat tgacatcgaa gtgtataaca cagcaaaagc aaggtttttg caaactcttg
    18961 aaagcccaaa gttcgataaa atcaaacttg aaaaactccg ttataagaat tcgctggcat
    19021 caccgcataa acatgcctgg aatctctggt actccttcta tcgcctcaag cggagggtaa
    19081 ggcggtatct tggagacgaa tccagttaac cagcacggca acctcatcac cccgtttacc
    19141 attctcccaa taacactggg agaaaatttt ttcaaaaagg atctattcac atgagaaaaa
    19201 atcccctgtt cctcctgctc tctctttttg tgattctcag cctcttcctc gcctcgtgtg
    19261 gcggcgaaga aggtgaatat tacacagagg aagccaccaa tgatgaatac ctcaccgaag
    19321 aaccctttgc aacagaagca cccgcttcag aatcagaagg tggggcacct gccgccacca
    19381 acctccctgg tgaagttgtc tacgatctcg gctttaaccc agtggacaat ggattcagct
    19441 ttagcaatta cggcgatgac atccccgcca caaatctaac agcggatgaa gtgcgccgca
    19501 tgttcggcga ccaggtctgc gcccgcctcg atggagatac ctgcaccctg acacctcctg
    19561 ccgagcaatg gatggaacag atcaatggcg ccatggcagg cgggcattgt gaaggcatgg
    19621 ctgtactcag tttaatgatg tacaccgacc aggtctcacc atctaacttt ggaggctcca
    19681 cagccagcga acttgatatc aatgatgagg ctctccagcg cgaaattgcc tattggtggg
    19741 caactcaagc tgtagcgcct actaacacca gcattatcaa aggcactcct gttgaaatcc
    19801 ttgagatcgt ccaacaaatg gatgcaaaag gagaaaccta caccatcgga atttacaaag
    19861 aagatggttc tggtggtcat gccattactc ccattggcgt ggaagacaag ggcaatgggt
    19921 tatatgccat tcttgtgtac gacaacaact accctggcga aacccgcgaa ctgtatgtgg
    19981 attcccgcga caacacctgg ctatatgagg cctcgatcaa tccacaggtt cagtctgagc
    20041 tttacactgg caatgccgat acccaaaccc tcgacctcac ccccacttcc gcccgcctca
    20101 acacccaaca atgtcccttc tgtaatggca gtggagtttc aagttttaca ggcggcaaac
    20161 tcacctcacc gagtcttcag ggcagccaga tcaaccagat ctttctcgat ggcgaaggtc
    20221 acattctcat taccgatgaa aacggcaacc gtctcggcta tgtggatgga aagatcgtca
    20281 acgagatacc cggtgcaacc tactctgcat atcgcatgga agccagcgga aacgcacccg
    20341 aacccattta ctcgctccca gccgatttgg atgtgaccgt ttcaattgac ggctctgcct
    20401 taaccgaaga aacgctcacc gacctcatca tgatcggcgc cggtttcagc atcggtgtgg
    20461 agggcatcta cctcgaacct ggtcaggtgg atacggccta cttctacccg tctgaagaaa
    20521 ccattgccta cgaaaccgaa gcggacgaat ccccgttcat tgtgatcggt gtggaaaatc
    20581 ccgatgccga agcggactat tacttcgaag tacaaggcgc tgatattcaa ggcggcggca
    20641 tcatcacggt ctatctcgac accaaagccg gtgacctgct catcaatgcc gaaaaactca
    20701 ataacgaagg cttcttcgat ttctatctca cccgcatcac cgacgaactc gaagaggaat
    20761 ttgccgccga agagatcgcc ttgcaggaag gcgccattgt ctacgtcaac tacgccgaat
    20821 ggaatgacgc caaccccgaa ggcatgtact ttggtgttga ccttgacggc gatggaacca
    20881 ttgacgacga atacgtggtg gacgattcgc aataaattcg gaaaggctgg ctgcgcaaag
    20941 tcgtactgag caaagccaaa tatctctcga ccagaaaact gcccactcca gtaaaaacgg
    21001 gtgggcagtt tcacctcttg acaaatagac gtttgtctat ataataaaca acagaaagcc
    21061 atcaaaatga aattcaatcc tgttctcttt gccaaagcca tctcagacga aacccgccag
    21121 aagatcatga acatctgctg ttgcagttca ctttcggtca gcgagatcgt tgagaagctc
    21181 gatgtttccc agcctacggt gtcacaccac ctcgccattt tgcgcgaagc ggacttggtc
    21241 actgtgcgtg aagatggcaa gcaaacgttc tactctctca accaggataa tgtcgcttat
    21301 tgttgcggcc agctcatggt caagtttgca ccggagtctg tcgccacaga aaatttgatc
    21361 aagaccatca aggcctgatc gggaaatgtc ttcggccgag gcatcgtccc gttttttttg
    21421 gcaaattcca tagacaaact tctatatgaa aggagaaacc ccatgaacga attaccagtt
    21481 gtaaacaatg aatgctgtga aggcggctgt gaaagctgcg gttgcggctg ctgctagtgt
    21541 acgaaccctg ttcaccgcat ggtgaatagg gcgcatttat cggtaaattt ttttacaaaa
    21601 acaaatagac attcttctat acaaaggatc tgacaatgga ctttccatct ctacttcaat
    21661 acacgttcgg caaagttgtg gaaacctttt ctcacaattg gttcctgctg ctgctgagca
    21721 tcatcgccag cgctgcgctg aaactttatg ttgatgaaga tgccgttgcg cgttttctgc
    21781 gccgaaacac aaagaatagt gtgcttatgt ctacaggcgt tgccgtcgcc acaccgcttt
    21841 gctcgtgtgg caccacggcc atcgtgctgg gaatgatggc ttccacgatc ccctgggcgc
    21901 ccatcgtggc ctttttggtg gcctcaccgc tcacctctcc gcaggagctt ttttatagcg
    21961 ctggcctttt tggctgggat tttgccatcg ccttttttgt cgcatccatt gtgcttggcc
    22021 tcgcaggcgg agccattgcc agttttgcag aatcgcgcgg ctggcttgcg aaccaggccc
    22081 gcatggctgc tcccaaattt gccgccacct caatgggaat catgggcggc ccggtcccgc
    22141 tcgacctgcc ggtgatcatg cagcctgtcg caaaaacaga aaagccaacc gtcacctttc
    22201 aaatgttcat acaagaaatg tggaccgtga gcaggcgctt actgcctctc ttcctcggct
    22261 tcactttcat tggttacatg ctcaacggaa tgatccccgc cgcatggatc accagcctgt
    22321 tcggcagcgg gcatgtctac agcattccac ttgccgccac actcgggctt ccgttttaca
    22381 tcaacaccga agcatccctg ccgctcgtcc gcgcaatgat cgatagcggc atgagtcagg
    22441 gtgcggcgct tgccttcctg atcacaggag caggcacatc cctcggcgcg ttgggcggag
    22501 cactgaccat tgcccgctgg cgcgtcatcg gcatcgtcgt cggcacactg tgggtcggtt
    22561 ccatcatcct cggctttgcc tacaaccttt tcattatgtg attttcaaca aggagactca
    22621 aatgacccaa tcccccactc ccattcacga aaccgttcgc gaacactacg cagagcggat
    22681 taaaagcagc gcttcctgct gtggaccttc tgactgctgc acaccaaaca acattctcta
    22741 ccccgaacag ctgctcacca ccgttcccag cgacgtggcc agcaccagct acggctgcgg
    22801 tgacccgatc acactcgcct cattgaagcc cggtcaaacc gtgctcgacc tcggctccgg
    22861 ggcaggcctc gattgcatcc tcgctgcaca aaaagtcggc gaaagcggac atgtcatcgg
    22921 tgtggatatg acgcccgaaa tgatcgaacg cgccacagcc aatgccaaaa aggtccattt
    22981 gaccaacgtt gagtttcgac agggatatct cgaagacctt cccgttgaaa gcaacacggt
    23041 ggatgtcatc atttccaatt gcgttattaa tctctcacca gataaggaaa aggtttttac
    23101 tgaagccttc cgcgtactcg cccccggcgg caaactggca gtctctgata tcgtcacagc
    23161 aggtgttctg cctgaacctg tgcgccagag ttttagcgcc tgggctggct gtgtagctgg
    23221 cgccgtgcag gcagaagaat acatcgccat gatgaattcc gtcggcttca ccgatatttc
    23281 catcaccccc gtcttctttg acaaggaatt cgtcgaatca gccattgctg aaatgaacct
    23341 ggacgtgagc aactatccac gcgaagaaat ctataaagcc atcttcagcg ccaagatcac
    23401 agcctacaaa cctgcctaaa agcaaaaaat cgttagccgt ttcttttcac cacagcgggc
    23461 acggagtgct ttagaagaac tccgctgccc gctgtggtcg tcccgcccat ttatgaatcg
    23521 ggctgtaatt tctgtatatt cttcaagttg aacacagaga tctgcctgat tccaagcaag
    23581 gtctgtgcct ccgtggtcaa ttccacccag taaaaactac gatgggctga atacggatcg
    23641 accttgaaga cggtgccgtt gtaccagcct tccttttcgc caaatttcga atcgagatag
    23701 atctttaccc gatcacctat ttgaatcgac tctgccattt cagcccatca ccagccgcgc
    23761 agcgatgtcg gtttgacacg gatcgccgtc gaatattcac tgctgaactg ttccagagtc
    23821 atccccaact ctttgatgcc tttgcgatat ttccgcaaat aagcagggac tttatgtgcg
    23881 ggcgacgttt tcgcatccaa tgtggccatg ccgatataaa tgaccacatc ctgatcgcct
    23941 ttctcatctg tgttgaaatg cagggaaaca ctgccatttt cacgaatgtg ctgaaccttg
    24001 tgcgccttcg gctggctgaa gatcaaaaag gaatcttcat cccaaatgaa ccacacaggc
    24061 ctgggttggg gaacgtgttt tgcatccacc gtggtcagcc agatgacata ttcattcttg
    24121 atcatctttt tcgccttgcg ttcaagtttc accttcgaat cgctcatcat tgcctcctgt
    24181 gaaattggat gattattttc gacttgcaaa ccaaaacacc cccgcactga cagccatggt
    24241 aataccgcca ttgatcgccc actgcggatc acccgtcatg aaactgcctg gcagcacatt
    24301 gatgccttgc aaaacccagg tcccgcccgc cagaaaaaac aaaaccgcca atccttttac
    24361 aacattcttc accatagata atcctctctc tattcactac tcactactca ctattcacca
    24421 ctcaccactt actattttcc accctaccct gccaacgtca tcaacactcg aaatgcaaga
    24481 tacacagtca ccatatcctc tgcggaaaat tccacccgcc gatccactgt ccgcacggca
    24541 ccaggcatca gcgcggcttt atccgccatt tcagattgag cgaactccaa tgtcattttg
    24601 atgggtttag aaactttgaa cggctttgtc tttcttccgc tgcgcaggtt gatcaccgcc
    24661 cgtttggcgg cttcctcgat caaactggca gatgccgcag gggtcaaaca ttcagccgcc
    24721 atgcgcccaa cagccttctt cacttgtgca gtttcaacct cactgccaaa aaattcacgc
    24781 acttctgcac acaaggtttg gtcgcccgac gccatgatga tcgggacatc gaaatgcccg
    24841 cacaccgcac cattcaaacc cacttcccca aaagccctgt cattgaccca aactccagag
    24901 acactctccg accacgtatg gtcgagaatc gcatcggccg cccccgtgcg cccgtgatag
    24961 ccgatgaaca tcacgccatc cacgccgtca tccacaccct gcaccatcga aagcggagca
    25021 ggcgaaccag cgttcaacat ggcacgtgca tccaactcct cgatcaaaat atttctgccg
    25081 ctgttgtgac catccgtcac caccaccgat gtggctccgc cagcaaacgc cccgcgaatg
    25141 gcggcgttca catctgcagt catcaatttg cgaaaacggg catattcggg gtgcttgggg
    25201 ttgacctgat cccaatgcac cacgcctgta ataccttcca tatctgctgc aattaatatt
    25261 ttcatgaaac tccctacggt tttaaagccc gtgttgcgat gtaggtcggg gtgaattcgc
    25321 tgatccgcag gcccgtatga tgatcttcgt acatgtcaat gagatgaaac cccgcctcca
    25381 tctgacctgt caactggtcg gtgagcgaat ggccaaactc agcgggacgg tcttcctgct
    25441 tcaatcgttc catgccatag gtttcgatat ctgaaaacgg aattttatgt ctcacgttca
    25501 aaatgccgtt atccatgtct tcccaatcaa aaagatagaa caatggattc atgaatcctg
    25561 ccagtaatgt tccgcctttg cgcaacacgc gaaacgcctc tttccacaca gggcgaatct
    25621 cgtgaatgaa caaatttgag acggggtgaa agaccaggtc aaaactttca tcggcaaata
    25681 ccgagaggtc gcgcatatcc ccttccactg tgcggatgtt caacccatcg cggtcggcta
    25741 cctcgcggtc gcgttccagt tgggcgggag aattatcaaa aatggtgacc tcggcccctg
    25801 ccgccgcaaa gacaggtccc tgctgaccgc cgccgctcgc cagccccagg attttcaatc
    25861 cctgcatggg cgggaaccat ttcttcggta cgggaatatt ctgcgtcagc aaaacagacc
    25921 aatccccttt gcgggcattg gcaatcactt caggagaaac aggcaccgtc cacgggtttt
    25981 taccggaacg gacctgatta tcccaagctg cgcggttgta agaacggaca tcatgatttg
    26041 catccataaa acctcacctt gttcgaattt ttgttacaaa gattttacat ggtggcaatg
    26101 ggggcataaa acctatccgt acaattacaa cataggagac caccatgcgc aagtatctat
    26161 ttatcaccct tctcaccctg ttcgcgttat cttgcggaac gcccgcagca gcggcaggcg
    26221 aatcactgac agcggcggat ctgcccgttg aaacggtcac ccagcctgcg gttaagaatg
    26281 aatcgccaaa gtctgccacc acttccacaa ccatgctgta cgtctcggct acgatgcata
    26341 ttgaatcaca acctgattcc tggccgaagg atgtagatgc ctttctggcc ttcatccagc
    26401 aattgaccga tgccggtgtg cgcctttcca tcggggccga tgtgggctgg ttgacaggcg
    26461 gcatacgtgt gcaggagatc atccagcgca ccagcgccat gggtgtgcaa tgggatgttc
    26521 acgcgcataa acaaagtgac cgcgccaaag cggcctacct gctcgcaaag tacggagcca
    26581 cacccaccag cgttatcagt ggaatgcaga tcagcgagtt cgacaaggca cggaaacctt
    26641 cgagttacaa gggttataca tggacgccga aagtggtttg gggtggggtg gcttgcatag
    26701 gtcatcgccc gggctgtgac gatacatcga ttgtgttgta ccgcccgacc agcagtgcca
    26761 aatataccgt gcatgaccca aagggtaaca ttgtgcgtgt ggggaatacc gatcatcaac
    26821 tggcaacagc cgaatcactg cttgcacaga tcgaagctgg agcatattct gtgccgatgc
    26881 tcagtttcac aatcatgatc gagcctgaga ccttgctgat cgtcaattcg gacgacggcc
    26941 tcactgagat acttgccttc gtggagcagg tgaaagagaa tccaaatctc cgctttgcaa
    27001 cgattgaaga aacagcacag gcctgggttg cggcgggaag tgtgccaatt caaattccgt
    27061 agcccattaa atggtgagag gcgggctgcg tagacccgcc tctcttgtga ggggaacaac
    27121 aacttgctaa tctgcagcgg ctggttcaac cgccatagat acgggggaat ggtggatccg
    27181 atccaaaaca atgtaaccga gcatggcaac gccgccgatc acgccacatg caacccacag
    27241 ccagtttgga ttgatgtttt ccatgaccca gcctgccatg taagggccag ccgcaaaggc
    27301 gatgccccat gaaagacctg agatcgccat gtaacgtccg cgcatttctt cgggggcaaa
    27361 gcttgccaca agcgcctgct gaaaaggtga aacgaccatt tcgccgatgg tgatgatgat
    27421 catcgccacc acaaagagca ggtaggttga ggtaaatcca tacatggcaa agccgatcgc
    27481 gtataacgcg gtgccaagcg ccatcatcag catgggtttg tatttttcaa gtttccgtgc
    27541 cacccaaaat tgaaagaaca caacaatgag ggcgttcatg ctgagcaggt agccataatt
    27601 caattcaggc aagccgtggc tatcgcgcaa gtacacaccg agagtggtgt tcatgttcat
    27661 gtacaccagc gccatcaaaa tggtgacaaa aacgaatccc atgaacggaa tgtccttgaa
    27721 aacttttccg tagccagcga aggtttgggc aatggtttcg tgcttcgcat cagggtgagc
    27781 cttgggcttg gtctctggca gggcaaaaag gacgatcccc gccgccacca cactgatgac
    27841 tgcatcaatg ataaaaagca ggatgtagga ttttgcaatg agcaacccag ccgtggcagg
    27901 agcaatgatg accgccatgt tgaagatcac gcggatgatg ccatatcctt cggcacgttt
    27961 gttttcaggg agcaggtcgg caaccacagc ctcatgagcg ggtccgccaa tgctggaaag
    28021 ggtgcccacg atgaccgaaa ccgccacaaa gattcccagc gtgggggcat agcccatgcc
    28081 aagtgcgctc aatgccgaaa gcaaaaggct gaaaatgatc acgcccttgc gccccattcg
    28141 gtcggtaatt gcgccgccca acgcactgcc caccaacccg gaaatggaaa agatcccaaa
    28201 caaaatcccg actgtggcca tgccgatctc aaacttcttc gtcaggtaca gcgcaaagaa
    28261 cggatagagc atgaatccgc cgaagcggtc aatgaaaacg ataatgttgt acacccaaaa
    28321 cgcctttggg tattcatggt aaatagactt gagtcttgct aacattctct ctccttgaga
    28381 ataaaaactt cggaagcctg tcttcgtggt tcattaacaa caggcttccg aagaaaaagc
    28441 cgtaaaaata ttaatgagcg tagcgttgat ctgccgtgcg ccgattttga gcagggatct
    28501 catggcgttt caccaaatct tcaccttttt cgatcatcca ctgaccaaat ccgcgcagga
    28561 ggcgggtgga taatgtcggg tgatatacgc ctgccttcac cgtctgcccg agcaactgtg
    28621 cgctgttcat ttcacgttcg atctctctct gatgttcttc aaccaaatac tcttccaaaa
    28681 gcgggttcat gttaagcctc cttccaatga ctaccgatcc agtaaaaagg ccgaaactgc
    28741 cttaagtgtc ttctcgatac gttcacgatt taaagaatat tctttcgcgc cgcctgccct
    28801 cctttcctgt aaaaatccac tggctgtgag ttgaccgagg taacgcgaaa cactcggctg
    28861 gctgagtcgg gttgcatcca tcacctcgtt ggaacgcatt ttcccgtttt ctgcgatcat
    28921 ttgtaagatc tgcaaacgtg tctcatcggc aagagccgaa agtttgctga caatttccgc
    28981 acggtccaat tcaggtatct gcacatctga gccttcgggc aggtgtgctc caaaataaat
    29041 gtaaagatcg ttgccaaccc tggtcgtatg catatacggg ccaatatgag cgttcggcac
    29101 aaaggtcacc cggtcggcat tttcaagggt gtctgcccat tttgtttcct gcgcatcctt
    29161 gccggtcaca aatcgcaact gttcaaggcg tgtcatgtcg tcggtatcga gcttattaaa
    29221 agccctggcc gattcttcca acatggggcg aacacgcgcc cattctgcct gcaggtgatt
    29281 cttccaaaac caacggatgt gacccgtcac cagctgcttt agcgcggcag ggtcaatcac
    29341 atattgatag gcacgcgtct ccatttccac attggtcaga tcttcaccaa aacgatattt
    29401 cagaaagtcc acatagtttt cagcagaaga aagcgtctct tcccagttca cagactcgcc
    29461 ttgattggcc tttgcctctt ctgtgatgca gatcatttca taagccttga gcaggcgttc
    29521 acgaaatacc gaaggcggtg ttgcctccaa accatccaaa tatgcttcga agctgattcc
    29581 gcccttttgc ggcaaaatcg aataataaaa accgatggtc accaaaccat gacgaaactt
    29641 ctcctctgcc gacatctctc cacgggtctt tgaaacccat tcatgaatcc ccggctcatc
    29701 ctctgccttc gaaataagga gcatgctccc aaaagcattg agcgcgggct caagacctac
    29761 aagtgaagtt tcggttctgg ttttttcgag gttgagttca aaaggtgaca ttgggcgctc
    29821 cgctttattc tatttattca atttattgaa taatacacat attataatca cacggatgaa
    29881 tttgtcaagg gaattccaca aggaaaagaa aaactcccca ccgaaattgg cagggagttt
    29941 gggaatgaaa atccgcttct tggtctggac ctagggattg ggcgtgcctt cgacggccac
    30001 tccaacccag gtgaagacgt gcttgtcgct gacggcaccg gaagcaaccc aaatcggctg
    30061 cccctgttca tcagtccccg cctggcggac gggggtcacc caccagcgca ggacatgtgc
    30121 cacattatct ttcgggcgga aagaagttgg gacaatgtac ttggtatccg tcacgtaatc
    30181 ggtcaggcgg cgggtttggt ctgaggtgac atcctcaatg gtgatctggt aggcctcccc
    30241 atcacgcaaa acacccacgg aagcccattg cagggtcacc acgtcgtttg ccagggtgaa
    30301 ggctgctccg tccgcaggca gaagcaggtt cggggcgggg taaggaggcg gaatcgtcgc
    30361 agttggggtc ggcccggggg tggctgcccg cttacaaagc gggatcagca gcgacgatcc
    30421 aataaagacg ttatcggtgg gtaaaccgtt gaattcctta atagcctctg gcggtaccgc
    30481 gtaattcagc gcaatggtcg aaagagtatc cccatcctgt acggtgatcg gaacggtctc
    30541 acaactgatg cgggttgcct cggctgcttc gggtgtattt gttgcagcag gtggaacggt
    30601 cggcgtgggg tacgggatct tcaagacctg ccccacactg atcggacagg tggacggcag
    30661 attattggca atgatgatgc tctgaacgga aatattgaag ttgaacgcga tctgcgaaca
    30721 ggttccatca cccgcagaaa cggtgtaatc gaaaggcgga atattggtgg aggttggcac
    30781 ctgggtggga atcaacgtcg cagtggcggg ttgggtctct gtgggggtgg cgctgggcgt
    30841 ggcagtttca gcagtttcaa aggttccagg cggtgcgctg gcccttagag agaagaatac
    30901 gatggcagcg gccacgatga ccaccaccgc aagtgcgccc acggccgccg gcaaactcat
    30961 agtgatgacc ggcatacggc tggcctgcac cgatttttcc actttcttgg cggctttcgg
    31021 ctcaggtttg gcgttgaatt ccgtgccaca cacgagacag cgcattgcat tttcgctcaa
    31081 acgcgtaccg catgtaggac agacttttgt cttttgattg gggttggatt cttggctcat
    31141 acgaaaaaaa ttataccatc gaacaattac acgccttcaa cgacgaccat gttactgcgg
    31201 gcgtatttat gacgcaaagc acgggctggt gcatactcgg gagagttcac ccagttcttt
    31261 gcctgctcta cagaagggaa ttcaagaatg acaagccgcc tggcctgcca atcaccctcc
    31321 agggtttcgt tatagccgcc acgtgcaatg tactttccgc catacaattt caccgcttcc
    31381 ggcgccaatt tgacatactc cttgtacccc tctggatctg aaacttcaat atctacgatt
    31441 acataggctg tcattgtttt tttcctttta tggctccacc gttttgacgg aaaagcacaa
    31501 gttttgaccg cgaattttca gatcaaattc gcgaaaatcc gtgtaaaact gcattgagca
    31561 gggatttatt gcaggggaat ttattgtggc ggttgcggtt tgggcaatgt ggtaacaagc
    31621 accttgtcca cacggcggcc atccatgtcg atcacctcaa agcggaagtt attccactcg
    31681 aaatggtcag ccgcctgcgg gacgcgcccg agcgaggtca tcacaaatcc gctcagggtt
    31741 tcgtattcat cttcatgggg cagcgactcg aaatcgaaga tttccttgaa ttcatccacc
    31801 tccaacatgc catccaaaag ccaagatcca tcctgacgtt gggtggcctg cggttcctca
    31861 aattccatgg caccgacgat ctcctccaaa atatcattga tcgtgagcaa gccctgcaac
    31921 ccaccgaatt cgtccaccac gaggagcagt tcggtgtttt tctctttgaa cagctccaat
    31981 gcgcgtgaag cgaacatggt ttcagggatg aaatgtgctg gcttgagcag agacttcaaa
    32041 tccacagctt taccggaaag gctttccacc agtagatcac gcgatttgac gatgccgacg
    32101 atggtttcca gtgaatcctg ccgaacagga aaacgggagt aaggacattc tgcgatcttc
    32161 tcacggatat cctcaatgga atcgttgata tccaaccaaa ccacctcggt ccgcggggtc
    32221 atcagcgagt aaacacggga atcaccgagg ctgaaaatgc cctcaaccat atcctgttcc
    32281 gcttcttcga acacccctgc ctgcgtaccc tgatcgatca acaggtggat ctcttcttcc
    32341 gtcacaggcg gttcggaaga agggtccact cccatcaggc tcaaagcaaa gttggtggct
    32401 ttgctcatta atttaatcaa cggagaaaac gccttcgaag tgaagagcat gggccctgcc
    32461 acaacctgcg caatcttttc cgggctggta agtccaagcc gcttggggac cagttctccc
    32521 agccaaatgg tgaggatggt gatggtcaac acaacgattg ccagtgaaat tgattcgcta
    32581 aattcaccaa gatagggaat cttctgaagc gccagggcca gggcctctga aatagtggca
    32641 ccacccaccg ctcccgccag cacaccgatc aacgtaatgc cgatctggac cgtggataaa
    32701 aatatattgg gatctttgag cagtttaagc gccaactctg acgatttatt cccgtcattt
    32761 gcctgttgct gcaaacgcac cttgcgggaa gcgaccaatg ctgcttcaga cattgccagc
    32821 aatgcattga tcaatatcag aattagaatc aaaacgatct cgttaataat actcatggtt
    32881 tccttgtcca ccggttacag gcttaaaaac ttaaagccgc gaaaaaacct cgcagcaaaa
    32941 gtcatctttc gtcaattgat ttgaataaaa cttgataaat ggagacgcaa aaaggtctat
    33001 caaaatttct atgggaattt ctgacatggg gtttttcagg tcgttttctc tacttctatt
    33061 gtaacttatt gttgtttttt cttatcgagc ttcttctcaa tgtctttcag acggccatcc
    33121 atacgggcct gaaaccttgc caccataatc ggtaccacaa ggaccagcag aataagagca
    33181 caggtgaaag ccaattccaa aatgcttatg cgaaacatct ttattgaacc gtaatcccct
    33241 gcccatccag cacttcaccg accacccaaa cgggctgacc taattctctg gcacgagttt
    33301 caaaggcact cagcttttca cgcggaacac caagcaaaag cccgccgctg gtctgtgggt
    33361 caaacaaaag catttgggca ggttcatcaa cacccacatc gaagcggaca ttttcgccga
    33421 catggccttt attgtcaaat gcgccgccgg ggaaggtccc tttttcagca taaccacgtg
    33481 cgccgctcaa tagcggcacc ttcgaatttt cgatggacaa acgtaccccg gaagcctccg
    33541 ccatttccag gccatgacca aggaaaccaa agccggtgat atccgtgccc ccgcgcagat
    33601 caaattccaa ggcaagctgc cccgctgttt tattcaaacg cgacatccat tcaatggctt
    33661 ccgcgatatg cctttcttca gctttttcct gcttgagcgc agttgtcgtc acaccgccgc
    33721 cgaggggttt tgtcagcacc agcacatcac ccaccctcag accgcctttg ctgatcatct
    33781 ttcgcggatc gacaaaaccg atcaccacca acccgaattt tggttccttg tctttgacgg
    33841 tatgcccgcc tgcaatcacc acccctgctt cacgggcctt ctccgcaccg ccgcgcatga
    33901 tctcactgga gatttcaatc ggcagatgat cgggcagggc ggccacattc aaagcaagga
    33961 agggctgccc gcccatggca tatacatctg aaagagagtt ggcggctgca atcgaaccat
    34021 attcgtaagg tgtatccacc acgggtgtga aaaaatctgt agtgacgacg atcgccttat
    34081 cttcacttaa acgccacaca gccgcatcat ccggggaagc aagccccacc aaaaggtctg
    34141 gatatgcagc aggatcaaaa acattcttga tcggacgcag aacctgcgtc agcgcatcag
    34201 cgcttagttt agacgctcag ccagcgcacg ccgaaagcgt ggtcaggcga atttcgcgcc
    34261 cagattgagg aatcgacatt gtgctcctat ttcgaatcag cgggatgaag atcttattcc
    34321 gctttcagca gacagactaa agtctgcgcc ccttataaac aatatgacca tcggcaaaac
    34381 aaccgacggt catattgtac caatgaagcg tgatcgattt tactgtgtct gcaaagtggt
    34441 atcgggcagc ggcaaaataa actgattacc gctcggaatg aaaatggtct gcacacccgg
    34501 ggcaagtttc aaaatatatt gatactgtaa aagcgtgggc gtgagcgaag ccgccaacaa
    34561 ctgattggct tgtgcctcgg cctgcgcctg aataagccgc gcctctgccg cgccttgcgc
    34621 cgcaatcacc gcagcatctg cctgtccttg cgcaacctga cgggcttgtt cggcttcctg
    34681 ctttttctgt tcaacaacta attttgcctg ttgggcttgt tgctcggcaa tctgcttttg
    34741 ttcgacagcg gcagcatact cttcgctaaa atgaatatca cgaagcagga aactcaccaa
    34801 aaccaggtca ttctctttga atgcctctgt caacgatctg gtgatctctt cttccatttc
    34861 agcgcgcttg gtgctgacga tctcctcgac gctgtattgc gaaacagcat cgcggatggt
    34921 gtttcgtgaa gcggggcgca ccaattcatc ctgatatcgg ctctgccagt tgatatgcag
    34981 ttcaatgatc ttcgatggat caatggagta gatcaccgac gcatcaatca aaatttcctg
    35041 cccatcgttc gtacgggcac ggatcgaatc atcgccgaca atctgacctt ccgtaggcga
    35101 tgaagacatt gtataggtct ggcgcgagat cgggtacaaa gtgacctgct ctgcaaaagg
    35161 aatgatccag cgcaagccag gctgcaatga ctgttcacga taccctttgg aagaaaacgc
    35221 agaaatcaca atgccgcgtt cttcgggctg aatgaaaacg atccctgcac caagggtcgt
    35281 cagcaaaatc gctacaacga gcgatgccac tacccctgtc ccgaacccct tcgaaggctg
    35341 gttgcggctc gaacgaacac cgatcatcac cagcaaacca acgaaaacca gccatgcaaa
    35401 agaaccaacc gcttgaatta cacctgcaat attcatgact cctccgagtt attgtttggt
    35461 tgattataat catcccatgc acacaggcac aggactaatc accgaaatat atctggatgg
    35521 ctccgcccaa atggactgtc ctccaaatct gattccagcg ccggggcaat atctcctcgc
    35581 ccacgccagc ggatcaacct caccgctgcc tgtttctgtt tacttctacg actcagcgcc
    35641 gaaagggttt cgcttcgcgc cgcccctgcc ccctacctgg acaatcggaa cgcagcttaa
    35701 cttacgcggc ccgcttgggc atgggttttc cgtccctgcc aatacacgca aactggcgtt
    35761 gatcgcactt gaatcctcat ccgcccgcct gaacgggttg attcccattg cattgaaaca
    35821 gggtgccgaa gttgtgctgg tatgcgattc cgcgccgagt ggtttgcccg aagtcgtgga
    35881 agtgcagccg ttgcgttcac ttgaagaaat ttgcaaatgg gctgatttcg tcgcagtcga
    35941 ttctgcccgt gagactctgc cacaggtaaa aaaactgttc gagaaactga atcaggtgtc
    36001 ggcggtgcgt gaagcgcagg ttttaatcca ggcgccactg ccgtgtggag gtctcgcaga
    36061 atgcggcgtg tgtgcggttt catcccatca tccacatgaa tggaagatgg cttgcaaaga
    36121 tgggcctgta tttgaaatga atttctttat atgatgtttg tactttggcc ttcgtgacgc
    36181 ttggcactac tctttattgt aggaaaacga cataatcaac atggcgggca atcccgctaa
    36241 attatgtccg acttctcata taaatcaagg agttgcaatg gccgcaaaag gctggatcga
    36301 agtcagtgat acttattgta aaggatgcga ggtgtgcatt agtgcatgtc cgcagggtgt
    36361 gatggaaatt aacatgtcac acctgacccc gaagggctac caccccgcgc acacgttcaa
    36421 ggacggttgc acggggtgtg ctattt
//
LOCUS       DAOQJC010000027        36134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_512_length_36134_cov_9.361817, whole genome shotgun sequence.
ACCESSION   DAOQJC010000027 DAOQJC010000000
VERSION     DAOQJC010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 36134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36134
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_512_length_36134_cov_9.361817"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..960)
                     /locus_tag="PLE39_05820"
     CDS             complement(25..960)
                     /locus_tag="PLE39_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE67877.1"
                     /translation="MIETHDLSKQFHDFMAVDGVNLSVDSGQILALLGQNGAGKTTTV
                     RMLTALLNPTRGWARVAGYDVVKNGHAVRSSVGVLTEQHGLYMRMNAIEYLDYFGRIY
                     NLEPAIRKTRSDNLLEYYGLTEAAKRRIGEYSKGMRQKLALARAMMHDPGVLLLDEPT
                     SAMDPESARLVRDEIARLKSSQRTIVLCSHNLTEVESLADKIAIIYRGKILLQGSLEE
                     MKRDVLGAPEYEIRFSEAWDAGGLVLPEGVTLLSRTATSLKVRVERPQVSNPIMLQAL
                     SALGAPLLAFQEQARTLEQVYLKVMADAKGSDHVQ"
     gene            complement(1029..1829)
                     /gene="lgt"
                     /locus_tag="PLE39_05825"
     CDS             complement(1029..1829)
                     /gene="lgt"
                     /locus_tag="PLE39_05825"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNE67878.1"
                     /translation="MTEGLSLGPILLRWNGLLIACGMALGALLSAREAKRHGYDADLI
                     YYLFLPLAVWGTLGARLWHILTPPLSSVQLGLTTQHYLTHALDFIAFWLGGFGFPGAL
                     IGGGLALLYFAKKNELSFWALTDVLAPGLALGQAIGRIGNYFNQELYGLPTDLPWKIF
                     IDPAYRLSGFEAVNFYHPLFAYESLLSLGNLFLLLWLSRRFAETLAVGDLFFAYLGFH
                     SLARFLLEFLRLDVALVNGLNINQAFFAVTFLFSSVSLFWHHRRVREL"
     gene            complement(1831..2457)
                     /locus_tag="PLE39_05830"
     CDS             complement(1831..2457)
                     /locus_tag="PLE39_05830"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNE67879.1"
                     /translation="MKPTFTDQLRRIFKGILDPTGAFLNRSGLTPNAITLLGLAGTTV
                     GAYILSQGYMTIGGIVLFVSVLVDALDGTMARLRGEPSDFGGFVDSVSDRYAEFITFG
                     GLLYFFLTQADYPGVVATFLATAGSVLVSYVKARAEGLGFTAKVGLLTRVERYIVLIP
                     LLIFNQPFLAVVFIAVLGNITALQRILHVRAQGRERMKKAKENKEYLH"
     gene            complement(2454..4724)
                     /locus_tag="PLE39_05835"
     CDS             complement(2454..4724)
                     /locus_tag="PLE39_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNE67880.1"
                     /translation="MTDSSQPRNRQYRQAEPPPIAQNDWLDRFARFYLQFGRFARDIL
                     GVVLFAFALMSLLAVFESIFNQIRSGSETGYNTVMGGVILSFVASRLRLWLGVGSIFV
                     ILGFGYMGYWLIQRDRNEIAWGRLISLELASLLTLGLFAVLGNNDIIAAEAGWDGGRL
                     GWGLVTLAWRLMGKIGGSLVLFLLWIFTIITGFGLWTKIEAWLLKLAGEAPPVVVQTP
                     IPSEPVEQPVEEPEKPAAPKKKAPVIPAEFRTSLKAVDKKDEKPAKPRPRNEDLPPLH
                     ILLAESALRADERTINQTAGLIMKTLADFGIPATVIGYRVGPSVTQFAVQPGFIKKPG
                     TDEEDVQQMKVRVAQIASLEKDIALALSAQRLRIEAPVPGKPYVGIEVPNTHSSVVRL
                     KTLLESEAFNKVGAPLTVAVGRDVSGNPLIADLSRMPHLLIAGATGSGKSVCITSIAA
                     CLAMNNTPEDLRMVMIDSKMVELIRFNGLPHLYGKVETNLERILGVLRWVVVEMEHRY
                     RLLESAHARDLDTYNRKLTRKADGKPLPRIVVMIDELADLMMSAPDVTEHNLVRLAQM
                     ARATGIHLVVATQRPSTDVVTGLIKANFPARLAFAVASGTDSRVILDYTGAESLLGRG
                     DMLFLNPEVGNPVRAQGVMITDMEIERIISHWQKNGDGSEETPPWEKLLQEPGEDEDD
                     GLVEQAISIVRQSQRASASLLQRRLRIGYPRAARLLDQLEEMGVVGPSQGGGKERDVL
                     VSEEDLEEGRQEDLGK"
     gene            complement(4731..5729)
                     /locus_tag="PLE39_05840"
     CDS             complement(4731..5729)
                     /locus_tag="PLE39_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNE67881.1"
                     /translation="MSVQKNKHENPMPVIDEGWWSSVLMEESRYSSAHTPVRSAGNRS
                     EVRSDSKSVASQNDAAMDDPTEKKPATNWRQVKELYMSDQIVDLMVTGHNRGGLLVEG
                     GGLYGFVPFSHLIELAGKVENIDRDHDLEAYVGRSLHLKVIECVPEDDRVVFSERAAQ
                     SEPGKRAELFSALQAGQKVKGTVTNVTDFGVFVDLGGVEGLIHISELSWGRVSHPGMI
                     TKIGNSIDVQVLDLAPERCRVALSLKRLQNNPWESANAEFPEGSIQNATITNVLSFGA
                     FARLDAGVEGLIHVSEIPQANGRPLKEILSEGQPVQVRILHLDSAHQRMGLSMRLE"
     gene            complement(5799..7709)
                     /locus_tag="PLE39_05845"
     CDS             complement(5799..7709)
                     /locus_tag="PLE39_05845"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67882.1"
                     /translation="MINGWMEPLLAGIQALNQILTAGIAITAFSLFLYSLSFNLRDRV
                     ARSFAIILLCVVIVFTAEALQDEAMSSETVDLLLRLQWFGIVFLPAAYLHLSDALLVT
                     AGRPSRGRRRLAVRGMYVVSAFFLALLAFGYLLGPLVPDGKPAPHLQRTMWTEVFTLF
                     YVTAMVWAGVNFARAYSLMLTRSGRRRMLYLMAGATAPALGSYPYLLFGYGLAAEHPY
                     LFWSVATVINVLVGALVIIMAYAVAFFGVSWPDRVIKSRLFKWIMRGPVTASAALALM
                     TVIRRSGELLGSPYNPFVPFIVVVTILSLEHAITFAAPLWERWLFFGRDRGELQLLQN
                     FEERLLTQSDLQQFLESVLAAVRDHMQSPSAFVAALDDETLSPIVVAGNRAMLDQEGL
                     TEALEQIGEDERHEFQWGSFWILPLRHRRRPEMDQDEMPPLLGLLGVAKRDKQLIERD
                     QRDALWLLADRAALALQDRQLQQRAFQSLVDLQPQVEMIQRMRAAVRYDTRANLLTEP
                     LPEEADLANWVKDALTHYWGGPKLTNNPLIKLQVVRELAEKDGGNSANAVRTLLRRAV
                     DQVKPKGDRKFTTEWILFNILEMKFIEGRKVRDVASRLAMSEADLYRKQRIAVEAVAK
                     AILEMEMSRKEA"
     gene            complement(7767..8306)
                     /locus_tag="PLE39_05850"
     CDS             complement(7767..8306)
                     /locus_tag="PLE39_05850"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE67883.1"
                     /translation="MSEIQIRPTIANDLSRLMGFDHTATSDSVWQLELRRDTGQVLAS
                     FREVRLPRSISVAYPRNSFGLADDWAKRSMMYTACIGADPVGYISLLERGMDSMVWVT
                     DLVVDVAHRRQRVGSTLLAAAQDWASGRSYRRLGLEMQSKNLPAIRLAQKFGYEFCGY
                     NDHYYLTQDVALFFGKTLK"
     gene            complement(8334..9008)
                     /locus_tag="PLE39_05855"
     CDS             complement(8334..9008)
                     /locus_tag="PLE39_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67884.1"
                     /translation="MAEIYNFLASYEALIYIVLAIGGLFSFRWLWRSWRESQTAVFTM
                     EREFSSRRLGQAAAISTIIAVLFCAEFFVATFIIPGLPSTALIPTSTLDLISTPTGTL
                     SAEAMTQFANLPPQPASVSLTGCTPNQIMLTSPTPGQEVQGTITLVGTVNIPNFGFYK
                     YEVAPQGGSTWATISAGRETVVNGKLGEWITNALTPGDYQLRLVVTDNQGVSLPACVV
                     PVRVMP"
     gene            complement(9023..9859)
                     /locus_tag="PLE39_05860"
     CDS             complement(9023..9859)
                     /locus_tag="PLE39_05860"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNE67885.1"
                     /translation="MQNFITLEQIDSVAQAIKKQISIQPIVGIILGSGLNGLADSVRD
                     AVYIPYKDLPNFPVSTVHGHAGRFVIGELEGKPVFVMQGRIHYYEGYTMGQATLPVRV
                     MQRLGIPSMIVTNAAGGVHPDFEPGDVMLITDQLNLMGMSGLNPLMGPNLDEIGPRFP
                     DMSQPYDRAYCDLARAVAKENGLTLREGVYAGLSGPSFESPADLRFLRLAGADAVGMS
                     TVPEVIIARHGNMRVLGLSGISNKANLDGSTITTHEEVIEAGQVITPKVEKIIRGVLR
                     SL"
     gene            10264..>11222
                     /locus_tag="PLE39_05865"
     CDS             10264..>11222
                     /locus_tag="PLE39_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67886.1"
                     /translation="MIRWLNILFLFLFAVLAIRADYFIDAIILILISTTQAILATPLR
                     DRFIKFNLLQTCVALIGFFGGLYLILNGDQYAGINLTPYRFPLAAMLMLFPVIWMFFT
                     LSRNHKFNPLVSRLLVIPWLIWCAIFTPPKLVANLIAPAMLCMVVLFEGFIPWNKLAV
                     ARNNVLGRKVVMIISTLEMAMLIFLGALFIVIDNFAGENYSIFAATHEGAFLFFILIS
                     IAVYFALFLILVTVNGIMSKLMELDKDDDEDVQSETKASLWNTRLAHYIEPFINIQEG
                     VRIRLLAQTDQIGVLTQQVKSEKKRNAQLTLLLELSQQLENQL"
     assembly_gap    11223..11322
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11323..12906
                     /locus_tag="PLE39_05870"
     CDS             <11323..12906
                     /locus_tag="PLE39_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNE67887.1"
                     /translation="SSYHQRLMNLVQAGSQLSAMVEPAATTREIATIAREILQARFTF
                     VQIQQGQERNFTESAYSGEAPRLLGSLQESLDSEALIKMAFNAVQPFRVRDVRKYSST
                     SHLMIDNPVFRSMLAIPIRWHRLSIGVILAFGKQNEVFFNENDESLAELLSIQAAGAF
                     ESAWLQQELRTSLRTTSLLYHLSNKIIQVDNLKDAARDIAQTAHKLAKSITTGVILFN
                     AEGDMVAEVEISSEGIHGGTDHPMEIIRDVMVSGQLIYLSRGQSIIRACLPIQTPIRT
                     YGAVWMDMPENQGNKHAANPNDLQALVSQTAIALERSLLLVESQRQAIEIKAAYDKLE
                     STYDQTLAALMSALDARDRETEGHSLRVSHLAAKLGKALNFSHDQLKVLERGSLLHDI
                     GKIGISDTILHKPGPLNEEEWKIMKLHPDIGAKIVAGIPFLQDTIPLIRHHQERWDGT
                     GYPDALYGEEIPILARMFAIVDAFDALTSKRPYRERTMSTEEALDYLRSQSGILFDPN
                     IIEIFETLIKENQPDLLID"
     gene            12910..14466
                     /locus_tag="PLE39_05875"
     CDS             12910..14466
                     /locus_tag="PLE39_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE67888.1"
                     /translation="MKTKFSSFTPQENLISILPSIVAGILVLVTTVSVSLVYFPPQQA
                     FTRNVILVFGIVGSIYLAFYYILFSASVNKSRFTWSNVIISSATLSTLAFVIPDELDH
                     MLYTLVFIASLSTSLISTRAPAYSLVISFTSIYIISRFNTKLETYDWVIHGSQVIAAF
                     MVIETIQQLKKMAREQINKLEIINELSKQIVSTLDTKQVFTLLNAAFQNALEADSYFI
                     GVVEGEEIRLELLYDGGEYFHNVKLPRRGTFSSWVIDNQRELFLPDLRQKIVLEGVDV
                     VLVGQNQTSLSWVGVPMRGTHVDGIMAIASYQPNAFDRGDVELLFTIAQRAALALDNA
                     HHHAIAEEQARLDSLTHAYNHGYFLKLLNQQAQECLDNQQPLSLIMLDIDFFKQYNDT
                     YGHIVGDEVLVSLCRTIQMHIKSTDAVGRWGGEEFVIFLPNADGIQATQIAQRIRESL
                     AAHKIQNNEHANLPIPTISMGIAVFPTETDQTTKLIDLADNRLYKAKERGRDQIEPTA
                     AFWEELNKSS"
     gene            complement(14468..15508)
                     /gene="ltaE"
                     /locus_tag="PLE39_05880"
     CDS             complement(14468..15508)
                     /gene="ltaE"
                     /locus_tag="PLE39_05880"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HNE67889.1"
                     /translation="MEFVDLRSDTVTKPTPEMREAMAEAEVGDDVYMDDPTVNALQEK
                     AAEMLGKEDALFVPSGTMGNLLALLVHCQRGDEVIVGDKSHIYLNEAGGMSALGGIHP
                     RPLQNQADGTLLLDEIRASIQTEDVHHTITRLICLENTQNVCGGVVLSAEYLQAVGKI
                     AQENGLAFHIDGARIFNAAAALNISVKDLVAPADSVMFCLSKGLVAPVGSMLVGGRKF
                     INRARHLRKMLGGGMRQAGVLAAAGMISLEKMSTRLGQDHARAKKLFEGLQQVRGLKL
                     DAFGPSSTNMVYFDLADEVKLSVNQVVEEMKKHGVLVDWAGPRRFRLVTHYWVDDAGV
                     EKTLQALGAVLK"
     gene            complement(15588..16508)
                     /locus_tag="PLE39_05885"
     CDS             complement(15588..16508)
                     /locus_tag="PLE39_05885"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNE67890.1"
                     /translation="MSDRVEKFVYDGEKADRLDKFLVTCLPEFSRARLQGLIDEGFVT
                     INNVRAKKSGQSIDPGAEIEVRIPPPVPSGLVGEDIPLDIIFENDDLLIVNKPAGMVV
                     HPAAGHDSGTLVHAVLGYDPDLEGIGGEERPGLVHRLDKETSGLIVLAKNERAHRWLQ
                     DQFRLRTVEKTYLALVDGMPPTPTGRVEASIGRDPSHRKKMAIVTAGKGREAVSEYKT
                     LEAFKNHTLLEFHPHTGRTHQIRLHCQFLGCPIVGDSIYGRKNPTVDLDRHFLHAYRL
                     KIVLPGEKEPRVFTAALPKELEGVLTDLRG"
     gene            complement(16505..17110)
                     /gene="lspA"
                     /locus_tag="PLE39_05890"
     CDS             complement(16505..17110)
                     /gene="lspA"
                     /locus_tag="PLE39_05890"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNE67891.1"
                     /translation="MNRVVKDYLVLFGVGGTSIALDQWTKWLVRTNIEFGMQWLPDSL
                     MWLSPYARIVHWYNKGAAFGMFQNGNIVFTILAFIVIGAIIYYYPRVEAGDWTLRLAM
                     GLQLGGAAGNLIDRLRVGQVTDFISVGTFPVFNIADASISVGVAVLLLGVWLNERKEK
                     LNAAQQSTPEGEQPAEAVLNGVGPVDGVAGGEQPKSNGGQS"
     gene            complement(17139..18254)
                     /gene="alr"
                     /locus_tag="PLE39_05895"
     CDS             complement(17139..18254)
                     /gene="alr"
                     /locus_tag="PLE39_05895"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNE67892.1"
                     /translation="MRPTFLEVNLTQLKQNLENIRAKVAPAKVLVVLKANAYGHGVDG
                     VAPFIAPFADYIGVAIAEEGIHLRKMGIATPILVMGGTLPQQLPDFFEYDLTLAASSL
                     DLLTAAEQLAASTRKRLKIHLKVDTGMERIGVREYEADSLIELALSCSHLDLEGIFTH
                     FANSEVMSLQKNMTPLSLQLERFEEILALFEKRGAPQPRIRHTANSGAILLHPESYYD
                     MVRPGVLFYGVYPGRDVEKTIEVRPALTWRSKVVYSKITQPGRGVSYGSLWQAEAESR
                     VVTVPCGYADGYFRRMTNQARVNINGRLYPQVGRICMDQFMVNAGEDDVKVGDEVILL
                     GGGITAEDLADWTGTNEYEVMTNISARVPRVFTRAVQ"
     gene            complement(18281..18733)
                     /locus_tag="PLE39_05900"
     CDS             complement(18281..18733)
                     /locus_tag="PLE39_05900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE67893.1"
                     /translation="MPNTIRPYKTEDFEAVTRLWFDAQTVAMPEMMARMGYEFSEAQE
                     YFRNIVVNENQIWVYERDGEPLGFLAIQGELIDRLYVHPAWHRQGIGQALLNKARELL
                     PKHIWLYTHTANRMARAFYEKNDFIAEGFGSSPPPESEPDVEYHWRAK"
     gene            complement(18969..19394)
                     /locus_tag="PLE39_05905"
     CDS             complement(18969..19394)
                     /locus_tag="PLE39_05905"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HNE67894.1"
                     /translation="MDFSSINWLAVVVCVLASMMIGGLWFGPKTFFPIWWKAVGKADV
                     QAPGSGTSMAIVWGLIVVSSFVQAMFMALMVNAMGSLSGGATLGSGALTGFLLWLGFV
                     APSSLTNKLFADRIKAWMIEVGNHLVTFVVMGAILGAWH"
     gene            complement(19577..20704)
                     /gene="dnaJ"
                     /locus_tag="PLE39_05910"
     CDS             complement(19577..20704)
                     /gene="dnaJ"
                     /locus_tag="PLE39_05910"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNE67895.1"
                     /translation="MSNQDYYETLGVGRNASDDEVKAAFRKLARQYHPDVNKEPDAEE
                     KFKEINEAYGVLSDSDKRARYDRFGKEGLGGMGSGGFHDYSADFGDIFEDLLSGFGFS
                     SGRRSRNSTRPRRGRDLQMAVSLTFEEAVFGVDKDIEFEREETCSRCNGNGAEPGTSP
                     VKCSTCNGQGEVRQVRQTFLGQMVQTAACPTCNGRGETISSPCKTCRGGGLERKHIKK
                     KVQIPAGVDHGTQIRLNGEGAPGVYGGPSGSLFLVLDVKQHQFFKRRDNDVVLNLDIN
                     VAQAVLGAEVDVPTLDGEEKLKIPAGTQPGKVITMKGKGVPYLRRKDRGNQLVIVNVA
                     IPTKLTKEQHQLFEKLAESLGTSVKPQEKGFLDWLNEALGG"
     gene            complement(20781..22661)
                     /gene="dnaK"
                     /locus_tag="PLE39_05915"
     CDS             complement(20781..22661)
                     /gene="dnaK"
                     /locus_tag="PLE39_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNE67896.1"
                     /translation="MAKIIGIDLGTTNSVAAVMSGGEPIVIPSAEGERLVPSVVAMNK
                     NSERLVGRVARNQAITNPQNTIFSVKRFMGRKSDDPEVERTRKRVPYAVNAADNGDVR
                     VRLGERDYSAPEVSAMILAKIKADAEAYLGETITQAVITVPAYFNDAQRNATKDAGKI
                     AGLEVLRIINEPTASSLAYGLDKKKNEVIVVYDLGGGTFDVSILDVGDGVFQVRATSG
                     DTFLGGDDFDLRIMDYLIEQFKKDNGIDLNNDRQALQRLKEASEKAKIELSTVMQTEI
                     NLPYLTADANGPKHLVMTLSRAKLEQITADLVDRTIAPVKQALSDAGLNAGDINEVVL
                     VGGMTRMPAIQDRVRAFFGKDPHKGVNPDEVVAIGAAIQAGVLGGEVKDILLLDVTPL
                     TLSIETLGGVATPQIERNTTIPTRRTQVFSTAGDNQTQVEIHVLQGERPMANDNKSLG
                     KFILDGIPPAPRGVPQIEVTFDVDANGILKVSAKDKATGRSQHITITASSGLSDSEVE
                     KMRKDAEAHAEEDRKRKDLIEARNNADNTAYAAEKALREFGDKIPADVRADVEAKAAE
                     VKSAAQGEDLEKIKAATEALGLSIQKIGASVYEQGQAAGGGEAGESSSAGPDVVDGEV
                     KE"
     gene            complement(22746..23291)
                     /gene="grpE"
                     /locus_tag="PLE39_05920"
     CDS             complement(22746..23291)
                     /gene="grpE"
                     /locus_tag="PLE39_05920"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNE67897.1"
                     /translation="MADEKDKQEEIDETQPSAEEMPVENGQASAEREALINQLKEAEA
                     RVIEYKDGWARSQAEFQNYKKRIERDNELTYASMKGDIIKKVLPALDDLERALQNRPA
                     DDAWASGIELIARKMQNILEGEGLKRIDAKGKPFDPNFHEAISHEPADGVESEHVIDV
                     VQNGYMLGDRVIRPALVRVAQ"
     gene            complement(23359..24396)
                     /gene="hrcA"
                     /locus_tag="PLE39_05925"
     CDS             complement(23359..24396)
                     /gene="hrcA"
                     /locus_tag="PLE39_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNE67898.1"
                     /translation="MPELTERQRTILQLLIRDYIETAQPVGSKRLVERFHLDLSPATV
                     RNEMSALTEMGYLRQPHTSAGRVPSEDGYRAFVGEMIHKAELPASVQNTISHQFFQAR
                     PDMDQWMTLAASILANQSQGVSVVTAPHAETIRFKHIELISTQGRQVLMVLVMDGGEV
                     NQQILTLAEIVTQERLSQAAAHLNSLLAGLSMDSIAAVSSRADALEQDIITILLQDMK
                     RTSESVSGEIYTDGLTNVLDEPEFAESDEARWALRLFEERSTLQGLLARASSNSSVGG
                     LQVLIGGSGGWDELRQCSMVVARYGVPGLLTGTLGVLGPMRMSYARTIPTVRYVAGLL
                     SDLVNDTISGE"
     gene            complement(24530..26644)
                     /locus_tag="PLE39_05930"
     CDS             complement(24530..26644)
                     /locus_tag="PLE39_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE67899.1"
                     /translation="MILENETLLHNRYRILENLGQGGMGSVYRAVDENLSVDVAVKEN
                     LFTTEEYARQFRLEAVILANIRHPNLPRVSDHFVIAGEGQYLVMDFIEGEDLRQRMER
                     LGALPEDEVILVGAAICDALTYLHARKPPILHRDIKPGNVKISPDGHIFLVDFGLAKI
                     MQGNQATTTGARAMTPGYSPPEQYGTARTDARTDIYSLGATLYAALTGVIPEDGLARA
                     MDNAQLTPLRKRNPDVARRLAAAIEKAMEVDPADRFQTAEEFKLAMLNAKSRTQQLLG
                     TYTIAPPPSEAERKSDPVKAPNIIKHSSAALEDQEFVSPRKKLLERNRRNMRALVGGL
                     LAVGFVVFLGIMLFVPNAMPESVRRLFPFLPTTEPTTIPTEIASVPPTPVAVVVDEPT
                     ATLVVSTPVQVTPTSSVNQAVEPTPIGADYLQIAYVSTRTGLPQIYLTNILTDQSIAV
                     TNMTGGACQPAWAPDGSKLVFISPCAVKKDTYPEASLFIINSDGTGLQPLPTALGGDF
                     EPAWSPDGKSIAFTSLRDGYMQIYSYDIENDKIQRLVETDATTAARQPAWSPDGRQLV
                     FSLKRVATYELWLMSSLGANERLLFTSGNVFSNQYPVWTPDGKFILFSQRRPEEATFP
                     NLFSHKIGDNNDAALKLTLSVRSVEDVDYSPGSFWIAYESGGDRGYHVYYSTPAGGNL
                     TRVTEDVTFDDFDPAWRPISTP"
     gene            complement(26893..29142)
                     /locus_tag="PLE39_05935"
     CDS             complement(26893..29142)
                     /locus_tag="PLE39_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE67900.1"
                     /translation="MTLEKNTLLHNRYRIIEILGQGGMGSVYRAIDENLGVAVALKEN
                     LFTTEEYTRQFRLEAVILANLRHPNLPRVSDHFELGEQGQYLVMDFIEGEDLRFRMER
                     LGTITEEDAVQIGAAMCDALAYLHSRKPPILHRDIKPGNVKITPDGHIFLVDFGLAKV
                     YQGASQATTTGARAMTPGYSPPEQYGTARTDPRTDYYSLGATLYAALCGVIPEDGLAR
                     AMDNAQLTPLRKRNPKISRRFAAAIEKAMAIDPSDRFQNAEDFKKALLGSSSKSQSRP
                     GGYTVTPPPAEAFLPNDPSEVVAAKEKEKTPPRQMTGPVQPASAEEEQPFVSPLKKQK
                     ERERKRRNALMRFMAVLILLAVIGVLVFAPGVIPADLRAQIPFLAPTSTATSSPTSTA
                     LPTFTLTSTSTATDVPPTATKTKVPPTHTNTPPPTGNIPVEATLTNTPSVTSTPTLVQ
                     PSPTPSLSVTPIGGGSGQIAYASNRTGLPQIYLADIASQTATQITEMPDGACQPSWSP
                     DGQRLVFISPCKGMDEIYYQAALYIINVDGTDLTPIATVPGGDFDPAWSPDGKSILFT
                     SLRTGQMEIFMVKLDDLSNAIEVTKGAKSVESRQPSWSPDGKQIVYSVKRFGVYQIWL
                     MNADGTEPKQIVRSGVAFSDYLPTWSPDGSLILFNQRCATSFCLPYLMSISSTDRSVE
                     QGTLLQLGVTSVEDVHYSPDGFHLLFEGEEAGENNDIIYVTVTGANRVRITTDPGLDF
                     DPVWRPTPK"
     gene            complement(29184..30182)
                     /locus_tag="PLE39_05940"
     CDS             complement(29184..30182)
                     /locus_tag="PLE39_05940"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNE67901.1"
                     /translation="MQTLIEGGIALIIAIQSLGDWLIAPMKFFSYLGNEEFFLLVLPL
                     LYWSVDSALGLRVAFILVTSNLFNHVGKLLFAGPRPYWVSSDVRALWNEISFGIPSGH
                     AQIAASVWGMFAAYSKKNWVWVGSITVIFLIGFSRLFLGAHFLHDVLLGWLIGAAILW
                     AFVRFWDSAAAWLEKKSMGGQIMVGFIVSLIFIAIGFGASLFRSGFQVPETWIANAAL
                     AGTEVPAPLDPNSTFTSAGTLFGVVIGAAWIHSFGGYQASGPVAKRAIRYVIGLIGIL
                     ILWMGLGAIFPRGEGFIFYTLRFIRYTLIGWWVAGGGPWLFVRLKLASWNTGENSI"
     gene            30254..32638
                     /locus_tag="PLE39_05945"
     CDS             30254..32638
                     /locus_tag="PLE39_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNE67902.1"
                     /translation="MEKSSITNSNAESSARTSVWDWRAILLTIALIEISSSRLVITEW
                     VPYLYFTQTISLAGVILGFTLGYSIFSRKAATRIAVGYSLILLPAQLLNAVERTDWVW
                     PDLVVLFNRLFTSMAQFIKGQPVYDQLFFISLVTAGYWIIGISAGYWLTRHRDFLNVI
                     LPSCLAMLVVQIFDPTESRRMWALAFYLFAALLLLGRLYFLENQTYWRKTRFLLTAET
                     INNLERGALTVAAITVFVAWSIPGWIKQIKPAAKAWREISEPILERLSDAVSALESPY
                     AGSNSGGEFYSSELSLGQQAAEGDTPVFFVTVKESEFVPIRNYWKGRMYDLYINGRWT
                     TLSDSNTPFVPDTDQLEVEYPENRFEMEFTFTNNLTSQNLLYGPSETIWVSREGTVTA
                     TEIGSSLKDVTAWNATPGVSSGGRYTVRALIADPTQEDLRAAGTDYPEWVTERYLQVP
                     ENIAPALRTLAEEITAPYDNPFDKTQAITAYLRNEIEYTTEITAEPPENRDPVLWVLF
                     DYKKGFCMYYASAEALMLRSIGIPARMAVGFAEGTFNEIDERYVVTFEDAHAWPEVYF
                     PGIGWVEFEPTGNQLPIERAATRDVINPDATPDPVTAETPAANPLVPPLLEEPPNLTT
                     QQNSGATQADRIELYKTIALYSLLTMTVVFVVFAFRRYSLDDRLPVYLASRYTKSGNQ
                     PPRWLNRWLRWTTLSPIERTFQAVNLSLYLLGRPQPRHITSQERAKALIERMPSAEPQ
                     VLALLQEYQSTIYTPKMGSLTTARKAAALILLKTWQIRAKEALQSFDYRYNQLR"
     gene            32645..34354
                     /locus_tag="PLE39_05950"
     CDS             32645..34354
                     /locus_tag="PLE39_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE67903.1"
                     /translation="MNEQTRPTTVPDQLQTLSLQVGKIRIDLEESRLKLPKNTGEDLR
                     HIETSLMLISEKIDAFQREHNNMLALANIGQVVNSSLELDEVLRIVMDNIVRLTKAER
                     GFLMLRDDKGEMVTRMARNWEMESIHSSELTVSRSVVGRVIETGEPIITTNAQEDQRF
                     VGQESIVAFNLRSILCVPLKVKNDLIGVIYADNRIRAGIFADSERNLLVAFANQAAVA
                     IENARLFSSLKNTLEEVTGLKNLMDNIFASIASGVITADIQNQITLTNRAAERILGSS
                     SSDIIGHHLNETLASVSSELEPHLTEVRQTDKPIVDLEISHHLPNHGNVDWRFNLSPL
                     KDAGQKTQGIAIVLDDLTERKKLEAQRRLFERMVSPAVIEQLDPNSLQLGGKRTNITT
                     LFADIRGFTSFSENQEPEKLVQILNRYLAAMAEAVLVQAGTIDKFMGDAIMAWFNAPI
                     PQPDHTLRAVKTALIIRESVENLYKELPPEFHLGFGAGIHCGDAVLGLIGTEKRLEYT
                     AISDAVNTAKRIQENSARNQILISASAYERVSKEINARPHADMSVKGKTQPLGVFEVL
                     GLK"
     assembly_gap    34363..34462
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(34848..35954)
                     /locus_tag="PLE39_05955"
     CDS             complement(34848..35954)
                     /locus_tag="PLE39_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNE67904.1"
                     /translation="MNNIATQTIDLAIQIQQIPAPTFSEGQRAEFTRDLFLKENLKDV
                     SMDSLGNVYARLPGKQKKAKPLIVSAHLDTVFPTSISLQVKKEAGRIVAPGIGDNSLG
                     VAALFGILWSLRERNLSLQHDLWFVANVGEEGLGDLRGMRAVVERFGANVIGYLVLEG
                     LALGHVYHRAIGVRRYRISVKTAGGHSWSDYGKPSAVHELANLVTQFAGIRLPREPRT
                     TLNVGTIHGGTGINVLASESKCELDLRSEDPKTLLKLIHQVEDLLKDSDRPGVRVLAE
                     IIGERPAGEIPADHPLVKLAVDCTREQGLESALTSGSTDANIPLSSGIPSVVMGITTG
                     GSAHTPQEFMEVDPVEKGMEALVRFVELVGERSE"
BASE COUNT         9097 a         9893 c         8807 g         8137 t
ORIGIN      
        1 gccaccagcc agaccggtct cagtttattg aacatggtca cttcctttgg catctgccat
       61 caccttcaaa tacacctgct ccagcgtacg cgcctgctct tgaaatgcca gcaaaggtgc
      121 tccaagcgca gacaacgcct gcaacatgat cggattggaa acctgcggcc gttccacacg
      181 gaccttcaaa ctggtcgctg tccgagagag cagggtcacc ccttcaggca acaccaaccc
      241 acccgcgtcc caagcttcac tgaatctgat ctcatattcg ggtgccccca gcacatcgcg
      301 tttcatctct tccaatgaac cctgcaagag gatcttgccg cgataaatga tcgcgatctt
      361 atcagctagt gactccacct cggtgagatt gtgcgaacaa aggacgatcg tccgctggga
      421 tgatttcaac cgcgcaattt catcccgcac cagccgcgcc gactcggggt ccatggctga
      481 agtgggttca tcaagaagca atacgccagg atcatgcatc atggcacgag ccagcgccag
      541 tttctggcgc atacctttcg agtattcgcc gatcctgcgc tttgcggctt ccgtcagccc
      601 gtaatattca agcaaattgt cgctgcgggt tttccgtatc gcgggttcca ggttatagat
      661 acgaccaaaa taatcgaggt attcaatggc attcatacgc atgtacaggc cgtgttgttc
      721 ggtcagcacc cccactgaag aacgaaccgc atgtccattt ttcacaacgt cataccctgc
      781 cacccgcgcc caaccacgcg tcgggtttag caaggcggtc aacatgcgga cggtggtggt
      841 tttgcccgcg ccattttgcc ccaacaacgc aaggatctgc ccagaatcta cagagagatt
      901 tacgccatcc acagccataa aatcgtggaa ctgcttgctt aggtcatgag tttcgatcat
      961 aggttttcct tcactggtat tgacgttatt gtattcccca ctttacaagg cctgccttca
     1021 cagagacctt acaattcccg gacacggcga tgatgccaaa acaggctcac acttgaaaag
     1081 agaaatgtca ccgcaaagaa agcctgattg atattcaacc cgtttaccaa tgcaacatcg
     1141 aggcgtagaa attccagcaa aaagcgggca aggctgtgaa aaccaagata tgcaaaaaac
     1201 aggtcaccga cggccaatgt ttcagcaaat cgccgcgaaa gccagagcag caagaacaaa
     1261 ttacccaggc tcaaaagcga ttcatacgca aacagcggat gataaaaatt caccgcttca
     1321 aatccgctta accgataggc tgggtcaata aagatcttcc acggcaaatc ggtggggaga
     1381 ccgtacaact cctgattgaa gtagttgccg atacgtccaa tggcctgccc caaggcaagc
     1441 cccggagcca gcacatcggt cagcgcccag aaagagagtt cattcttttt ggcaaaatat
     1501 aacaaggcca atccaccgcc gatcagcgca ccggggaagc cgaaaccacc gagccaaaag
     1561 gcgatgaaat ccaatgcatg ggtgagataa tgctgcgtcg tcagacccag ctgaacggaa
     1621 gaaagaggcg gggtcaaaat atgccagagc cgcgctccca gagtccccca aactgccagc
     1681 ggcagaaaaa gataataaat gaggtccgca tcataaccat gccgttttgc ttcacgtgcc
     1741 gaaagcaggg cacccaatgc catcccgcaa gcgatcagga ggccgttcca gcgcagaagg
     1801 atggggccaa gcgatagacc ttcggtcatg ttagtgcagg tactctttgt tctctttggc
     1861 ttttttcatt cgttcacgcc cctgcgcgcg gacatgcaaa atgcgctgca atgcggtgat
     1921 gttgccaagc acggcaatga ataccaccgc aagaaaaggt tgattgaaga tcagcaaagg
     1981 aattaaaaca atgtaccgtt ccacccgggt aagcaggccg accttggcgg taaagcccag
     2041 cccctcagca cgggctttaa cataagaaac cagcaccgaa cctgctgtgg ctaaaaaggt
     2101 tgccactaca ccggggtaat cggcctgtgt gagaaaaaaa tacaaaagcc cgccaaaggt
     2161 gatgaactct gcatagcggt cactgacaga atctacaaag ccgccaaaat cgcttggttc
     2221 accgcgcaaa cgagccatgg tgccatcaag ggcatccacc agaacagaga caaagaggac
     2281 gatgccgcct atggtcatgt atccctgtga caggatgtag gcacccaccg tggttcccgc
     2341 caaccccaac agagtgatgg cattcggcgt caacccgctg cgatttagga aggcacccgt
     2401 tggatccagg atgcctttga aaatacggcg caactggtct gtgaaagttg gtttcactta
     2461 cccagatcct cctgcctgcc ctcttccaaa tcttcttcgc tgacaagcac atcacgttcc
     2521 ttgccgccgc cctgtgatgg accgacaacg cccatctctt caagctgatc gagcaggcgt
     2581 gcagcacgcg gatatccgat tcgcaatcgg cgctgaagca gggaggctga ggcacgttga
     2641 ctttggcgca cgatggaaat ggcctgctct accagaccgt catcttcgtc ttcgccaggt
     2701 tcctgcagga gtttttccca gggtggagtc tcttcagacc cgtcaccgtt cttctgccag
     2761 tgagagatga tgcgctcgat ttccatgtcg gtgatcatga ccccttgtgc gcggacgggg
     2821 ttgcccacct cagggttgag gaaaagcatg tcaccacgcc caagcaggga ttctgcgccg
     2881 gtgtaatcga ggatgacgcg actgtctgtg ccggaggcta cagcaaatgc aagacgcgcc
     2941 gggaagttgg ctttgatgag acctgtcaca acatcggtgg agggtcgctg agtggcaaca
     3001 acgaggtgaa tacccgtcgc acgcgccatt tgcgcaaggc gtacaaggtt atgttcggtg
     3061 acatccgggg cagacatcat cagatcggca agttcgtcga tcattacgac gatgcgcggc
     3121 aggggtttgc catcagcttt gcgagtcaat ttgcgattgt aggtatccag atcgcgggca
     3181 tgggccgatt cgagcaggcg gtagcgatgc tccatttcaa cgacgaccca gcgcaacaca
     3241 cccaaaatgc gttcaaggtt ggtttccacc ttgccataca aatgcggcaa gccattgaag
     3301 cggatcaatt ccaccatctt tgaatcgatc atgaccatgc gcaaatcttc gggggtgttg
     3361 ttcatggcga ggcaggccgc aatggaagtg atgcagaccg atttaccaga gccggtggcg
     3421 cctgcaatca gcaagtgcgg catgcgtgaa aggtcggcaa tcagcggatt tcccgaaacg
     3481 tcacggccca cagccaccgt cagtggtgcg cccactttgt tgaacgcctc cgattcgagc
     3541 agggtcttca aacgcacaac cgagctgtga gtattgggta cctcgatgcc tacatagggt
     3601 ttgccgggca caggcgcttc aatacgcaaa cgctgggcag acaatgccag agctatatct
     3661 ttttcaagcg aggcgatctg cgccacacgc accttcattt gctgtacatc ttcttcatcg
     3721 gtgccgggct ttttaatgaa acccggttga actgcaaact gggtgaccga tgggcccacg
     3781 cggtacccga tcacggtggc agggatgccg aaatcggcga gggttttcat gatgagcccc
     3841 gccgtttgat taatggtacg ttcatccgca cggagggctg attcagccag aagaatatgc
     3901 agcggcggaa ggtcttcgtt acgcggacgc ggtttggcag gtttttcgtc tttcttgtca
     3961 acagccttca gcgaggttcg gaattcagca gggatgaccg gcgccttctt ctttggtgca
     4021 gctggctttt caggttcttc cacgggctgt tccacaggtt cactggggat gggcgtttgc
     4081 accacaacag ggggagcttc tcctgccagt ttcaaaagcc atgcttcgat ctttgtccac
     4141 aacccaaaac ccgtgatgat ggtgaaaatc cataacagaa acaggaccag ggaaccgccg
     4201 atctttccca tcaaacgcca ggcgagcgtg acaagtcccc agccaaggcg gccgccatcc
     4261 cagcctgctt ctgcagcgat gatgtcattg tttccgagaa cagcaaacag gccaagggtc
     4321 aggagagagg cgagttccag ggagataagt ctgccccagg cgatctcatt cctgtcgcgt
     4381 tggatcagcc aatatcccat gtatccaaac ccaagaataa cgaagatgct gcccactcca
     4441 agccaaagcc gcaaacggct ggctacaaat gaaaggatga ccccacccat gacggtgttg
     4501 taacccgttt cactgccgct gcggatctgg ttgaaaatgg attcaaacac agccagcaaa
     4561 gacatgagcg caaaagcaaa taacaccaca ccaagaatgt cgcgggcaaa gcggccaaat
     4621 tgcaggtaaa aacgagcgaa ccgatcgagc cagtcgttct gcgcgatggg gggtggttct
     4681 gcctgacggt actgtctatt gcgtggctga gatgaatcgg tcatgcggtt ttattcaagc
     4741 ctcatactta aacccatgcg ctgatgggcg gagtcaagat gcaaaatacg cacctgcaca
     4801 ggctggcctt cagaaaggat ttccttcaac ggcctgccgt tggcctgcgg aatttcagaa
     4861 acgtgaataa ggccttccac accggcgtca agccgtgcaa aggcaccaaa ggacaacacg
     4921 ttggtgatcg tggcattctg gatgctgcct tctggaaatt cagcgttggc gctctcccag
     4981 ggattgtttt gcaaacgttt aaggctcaag gcaacacggc agcgttcagg ggcaaggtcg
     5041 agcacttgca catcaatcga atttccgatc tttgtaatca tgccggggtg agaaacacgc
     5101 ccccaggata attcagaaat atggatcaac ccttccacac cgccgaggtc tacaaaaact
     5161 ccaaaatcgg tgacgttggt aacggtccct ttcaccttct gccctgcctg caatgcgctg
     5221 aaaagttcgg ctcgcttgcc cggttctgat tgtgccgcgc gttctgaaaa gaccacacga
     5281 tcatcttccg gcacacattc gatcactttc aaatgcaacg agcgccctac ataggcttcc
     5341 agatcgtggt cacggtcaat gttttccact ttccccgcca gttcgatcag gtgcgaaaat
     5401 ggcacaaatc catacaatcc gccgccctcc acgagcagac caccgcgatt gtgcccggtg
     5461 accatcagat ccacgatctg gtcactcata taaagttcct tgacctgacg ccaattggtc
     5521 gcaggttttt tctccgttgg atcatccatc gctgcatcat tttgtgaagc gacagacttc
     5581 gaatcactac ggacctcgga cctgttcccc gccgaacgaa ccggagtatg cgccgaagaa
     5641 taacggctct cctccatcag caccgatgac caccagccct cgtcgatgac gggcatcgga
     5701 ttttcatgtt tgtttttctg tacagacatc gtgaactcct tcccaacaca ataaatatga
     5761 gcggcaatgc ttcccaagca tacgctcgaa ttgcaaaatc aagcttcttt tcggctcatt
     5821 tccatttcaa gaatcgcttt tgcaaccgcc tcaacagcaa tacgctgctt gcggtacaga
     5881 tctgcctcag acatggcaag gcgtgaagcc acgtcacgca ccttgcgccc ttcaatgaat
     5941 ttcatttcaa ggatattgaa caggatccat tctgtggtga atttacgatc acccttgggt
     6001 ttgacctgat ccacggctct tctcaacagg gtacgcactg cattggcaga attaccgccg
     6061 tctttttcag ccagctcacg cacgacctgt aacttgatca acgggttatt cgtcaatttg
     6121 gggccgcccc aataatgggt tagggcatcc tttacccagt tggcgaggtc tgcttcttcg
     6181 ggtagcggtt ctgtgagcag attggcacgc gtatcatagc gaaccgcggc acgcatacgc
     6241 tggatcattt ccacctgggg ctgcaaatcc accaacgatt ggaaagcccg ttgctgaagt
     6301 tgacggtctt gcaatgcaag tgcggcacgg tctgccaaca gccagagggc atcgcgttgg
     6361 tcgcgttcga tcaactgctt atcgcgtttg gcaactccca acaaccccaa caaaggcggc
     6421 atttcatcct gatccatttc agggcggcgg cgatggcgta atggcaaaat ccaaaaactg
     6481 ccccactgaa actcatggcg ttcgtcctcg ccgatctgtt ccaaagcctc ggtgagtcct
     6541 tcctgatcga gcatggcgcg attcccagcc accacaatgg gtgacaaggt ttcatcatcc
     6601 agcgctgcca caaaagcgga tggagattgc atatggtcac gcactgcggc tagcaccgat
     6661 tcaaggaatt gctgtaaatc gctttgagta agcaggcgtt cctcaaaatt ttgaagcaat
     6721 tgcagttcac cgcgatctct gccgaagaaa agccagcgtt cccaaagagg tgcggcaaat
     6781 gtgatggcat gttcaagcga aagaatggtc acgaccacga tgaaaggcac aaagggatta
     6841 tagggtgaac caagcagttc cccactgcgg cgaatcaccg tcatcaatgc caaagcagcg
     6901 gatgcagtta caggaccacg catgatccat ttgaacaaac gacttttaat aacacggtct
     6961 ggccacgaaa cgccgaagaa agccaccgca tacgccataa taatgaccag tgcccccacc
     7021 agcacattga tgaccgtcgc cacactccaa aacaggtagg gatgttccgc cgcgaggcca
     7081 tatccaaaca acaggtaagg ataagaaccg agtgcaggtg cggttgcgcc agccatcagg
     7141 tacaacatac ggcggcgccc cgaacgggtc aacatcaggc tgtaagctcg tgcaaaattt
     7201 acacctgccc agaccatggc cgtgacgtag aacaacgtga agacctccgt ccacattgtg
     7261 cgttggaggt gcggggcggg cttcccatct ggaacgagcg ggccgagtaa atatccgaat
     7321 gcaagtaaag caaggaaaaa agccgaaaca acatacatgc cgcgtactgc aagccgtctg
     7381 cgcccacggg agggacgacc agccgttaca agcagggcat ctgaaagatg aagatatgca
     7441 gcagggagaa agacaatgcc gaaccattgc aagcgcaaaa gcagatcaac cgtttctgac
     7501 gacatcgctt catcctgcag cgcctcggcg gtgaaaacga tcactacaca aagcaaaatg
     7561 atcgcaaacg accgcgccac cctgtcacgc aggttgaacg aaagcgaata tagaaacagc
     7621 gaaaacgccg taatggcaat tcccgccgtc agaatctggt taagcgcctg gatccccgcc
     7681 agtaatggtt ccatccaacc gttaatcatg taacccttcc aacaaataat tcgcaagcct
     7741 ttggcccatt ttttgggcct ggatcactat ttcaatgttt tgccaaagaa caaggccaca
     7801 tcctgtgtta agtaataatg gtcattatac ccgcagaatt catacccgaa cttttgagca
     7861 agacgaatgg caggcaggtt tttcgactgc atctccaacc caaggcgacg gtacgatctt
     7921 cccgacgccc aatcctgcgc ggccgccaaa agggtactcc ccacccgttg gcgacggtgt
     7981 gccacatcca ccaccagatc ggtcacccac accatggaat ccatgccacg ctcaagcagg
     8041 ctgatatacc ccaccgggtc cgcgccgata caggctgtgt acatcatgga ccgtttggcc
     8101 caatcatcgg ccaaaccaaa agaattacgg gggtaagcca cagaaatgga tcggggcaag
     8161 cgcacttcac ggaacgaggc caacacttgc ccggtatcac ggcgcaattc aagctgccat
     8221 accgagtcac tggtggcagt atggtcaaac cccatcaaac gggaaaggtc attggcaatg
     8281 gttggacgaa tttgaatctc agacatattt tttccagaat acagtttttt ccatcaaggc
     8341 atcacgcgga ccggcacaac acaggcaggc agcgatactc cttgattatc agttacaacg
     8401 agtcttagtt gatagtctcc gggcgtgagg gcattggtga tccactcacc cagtttgcca
     8461 ttcaccacgg tctcacgacc agcagaaatg gtggcccaag tgctgccacc ttgtggggca
     8521 acttcatatt tgtaaaagcc aaagttggga atgttcaccg tccccaccaa tgtgatggta
     8581 ccctgcactt cctgccccgg agttggagag gtaagcatga tttggttggg ggtacagccc
     8641 gttaaactga ccgatgcggg ttggggaggc aggtttgcaa attgggtcat ggcttcggct
     8701 gaaagagtgc ctgtgggtgt ggagatcaga tccagggtgg aggtggggat caatgcagtt
     8761 gaaggaagcc ctggaatgat aaaagtggca acaaagaact cagcacaaaa caaaacagca
     8821 atgatcgtgg aaatggccgc ggcttgtccc agcctgcggg aggaaaactc acgttccatt
     8881 gtaaacaccg cagtttgcga ttcccgccat gaacgccata accagcgaaa ggagaataaa
     8941 cctcctatcg ccaaaacaat ataaatcaac gcctcataag aggcgaggaa attatatatt
     9001 tctgccataa atcaaactct tgctacaaac tgcgcaacac gccgcgaatg atcttttcaa
     9061 ccttcggcgt aatcacctgc ccggcttcaa tcacctcttc atgggtggtg attgtggaac
     9121 catccagatt ggctttatta ctgatgcccg agagacccaa cacacgcatg ttgccatgtc
     9181 gtgcaatgat cacctcgggg acggtggaca tgccgaccgc atcggcccca gccagacgca
     9241 ggaagcgtaa atcagcaggt gattcaaacg agggaccgct caaacctgca taaacgcctt
     9301 cacgcaaagt aagtccattt tcctttgcca ctgcacgggc cagatcacaa taggcacggt
     9361 catatggctg actcatatca ggaaagcgcg gaccgatctc atcaagattt gggcccatca
     9421 atggatttaa gccagacatt cccatcaggt taagctggtc tgtaataagc atcacatcac
     9481 ccggttcaaa atcggggtga acaccacctg cagcgttcgt tacgatcatg ctgggaatgc
     9541 ccagacgctg catcacacgc acaggcaggg tagcctgccc catggtgtag ccttcgtaat
     9601 agtgaatgcg tccctgcatg acgaagacag gtttgccttc caattcacca atgacgaacc
     9661 gtcccgcatg tccgtggacc gtggaaacgg gaaaattggg aagatcctta taagggatgt
     9721 acactgcatc tcggactgaa tcagccaaac cgttaagacc tgagccgaga ataattccca
     9781 caatgggttg gatggaaatt tgtttcttga tcgcctgtgc gaccgaatcg atctgttcaa
     9841 gagtgatgaa attttgcatg tgtgtatgtc cgtgtaagtt ttttgtaaaa ggctcatccc
     9901 catcggcaag ccaaatgtac attaagatta tatcagacct tgaagttggc tttattgcag
     9961 gggaatattg aaaaacatgc caacattttt ccattccaca acttttgaaa ggtaggtaaa
    10021 atcaaccctg ttatgttatt cacacaaacc acagagcaat ccactgaaca tgagttggac
    10081 ttgctcactt ctggaatcat tgggatattc ggagtgggtt tattgttgat tccctcacac
    10141 ttaaaccgat tcgttgcggg gatgtctact tgggttagtg tgattatggg cattatggcc
    10201 tttgcccttg ctttatcgct tcaaatcact tcaacttttc ggtcttcaca caaaccggcc
    10261 attatgatcc gatggttgaa tattcttttc cttttcctgt ttgcggttct tgcgatcagg
    10321 gcggattatt ttatagatgc cattatcctc atactgattt caacaacaca agcgatactt
    10381 gcaaccccgt tgcgagatcg ctttataaaa ttcaacctgc tgcaaacctg tgttgcatta
    10441 atcggttttt ttggcgggtt atacctgata ctcaacgggg atcaatatgc tggcatcaat
    10501 ctgacgccat acagatttcc actggcggcc atgttgatgt tattcccagt gatctggatg
    10561 ttttttacac tttccaggaa tcacaaattc aacccattgg tatcgcgcct gttagtcatt
    10621 ccatggctta tttggtgtgc aatttttaca ccgccaaaac ttgttgcgaa cctgatcgca
    10681 cctgccatgc tgtgcatggt tgtgctattt gagggtttca tcccgtggaa taaacttgcg
    10741 gttgccagaa acaatgttct ggggcgcaag gttgtcatga ttatcagcac gctggaaatg
    10801 gccatgctta ttttcctcgg cgcgcttttt atcgtcattg acaattttgc cggggaaaat
    10861 tactcgatat tcgctgcaac ccacgagggc gcatttcttt tctttattct gatatccatc
    10921 gcggtatatt ttgcattgtt cctcattctg gtaacggtca atggcatcat gagcaagttg
    10981 atggaattgg ataaggatga tgatgaggac gtacaatccg aaaccaaagc aagtctttgg
    11041 aatacccgcc tggcacacta tatagaacct tttattaata ttcaggaagg ggtgcgtatc
    11101 cgtttattgg cacagaccga ccagatcggt gtgctaaccc agcaggtaaa gagcgaaaaa
    11161 aagcgcaacg cacaactcac tctgttattg gaattaagcc agcagcttga aaatcaactt
    11221 gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcaagcta ccatcaacgc
    11341 ctgatgaatc tggtccaggc gggaagtcag ctctctgcca tggtggagcc cgctgcaacc
    11401 acacgcgaga ttgcaaccat tgcgcgggag atcctgcaag ccaggtttac atttgtacag
    11461 atccaacagg gacaggaacg gaacttcacc gaaagcgctt attccggaga agctccccgt
    11521 cttttggggt ctttgcaaga atctttggat tctgaggcgt tgatcaaaat ggccttcaac
    11581 gcagtacagc ctttccgtgt gagggatgtc cgcaagtatt cctccacatc acatcttatg
    11641 atcgataatc ctgttttccg aagcatgctg gccattccga tccgctggca tcggttgagt
    11701 atcggggtca ttctggcttt tggcaaacaa aatgaagtct ttttcaacga gaatgatgaa
    11761 tcgttggcgg aattgctgtc cattcaggcg gcaggcgcat ttgaaagtgc atggctgcaa
    11821 caggaactac gcacttccct gaggaccacc tccctgctgt atcacctaag caacaagatc
    11881 attcaggtgg ataatctgaa agatgctgcg cgggacattg cgcaaacggc ccataaactg
    11941 gccaagagca tcactacggg ggttatcctg ttcaatgcag agggtgacat ggtcgcagag
    12001 gtggaaatct catccgaggg tattcatggt ggaacagacc atcccatgga aatcatcaga
    12061 gatgtaatgg tttcggggca gctgatctat ctttcacgtg gacaatccat catccgcgcc
    12121 tgcctcccca tccaaactcc aattcgcact tatggcgctg tatggatgga tatgcccgaa
    12181 aaccagggaa acaaacatgc cgccaacccc aatgacctgc aggctctggt cagccagaca
    12241 gcaattgcgc ttgaacggtc tctactattg gttgaatcac agcggcaggc gattgaaatc
    12301 aaggcagcgt acgacaagct cgaatctaca tatgaccaga cccttgctgc actaatgtct
    12361 gcgttggatg cccgtgacag ggaaaccgaa ggacatagtt tgcgcgtcag tcacctggca
    12421 gccaaattgg gcaaggctct taatttttcg cacgaccagc tcaaggtatt ggaacgtgga
    12481 tcgcttctgc atgacattgg caagatcggt atcagcgata cgatcctgca caagcctggc
    12541 ccgttgaacg aagaagagtg gaagatcatg aaactccacc ccgatatcgg cgcaaaaatc
    12601 gtggcgggaa tcccatttct acaagacacc atccccctca tccgtcacca ccaggaacgc
    12661 tgggatggca cagggtatcc agatgcccta tacggcgagg agatccccat ccttgcacgc
    12721 atgtttgcaa ttgtggatgc atttgatgcc ctcaccagta aacgtcctta tagagaaaga
    12781 accatgagca ccgaagaagc cctggattac ctgcgctcac aatcgggcat tctgttcgac
    12841 cccaatatta ttgagatttt cgaaacactg atcaaggaaa accaacccga cttattaatt
    12901 gattaaagca tgaaaacaaa gtttagtagt tttacaccgc aagaaaacct gatctccatt
    12961 ttgccatcca ttgtggcggg cattttggtg ctggtgacca ctgtgagtgt aagccttgtc
    13021 tacttcccgc cgcaacaggc attcacaagg aatgttattt tggttttcgg cattgtaggc
    13081 agcatttacc ttgccttcta ctacatcctg ttttcagcct cggtgaacaa atccagattt
    13141 acctggtcca atgtcattat ttccagtgcc acactgagca cgctggcatt cgtcatccct
    13201 gacgaacttg accacatgct ttatacactg gtctttatcg cctccctctc cacatccctg
    13261 atctccaccc gcgcacccgc ttacagtctc gtcatcagct ttaccagtat ttacatcatc
    13321 tcaagattca acaccaaatt ggaaacctac gactgggtca tccacggaag ccaggtcatt
    13381 gctgcattca tggtgatcga gaccattcag caattaaaaa aaatggcccg tgaacagatc
    13441 aacaaacttg aaatcatcaa tgagcttagc aaacagatcg tctccacttt ggacacgaaa
    13501 caggtattca ctttgctcaa tgccgccttt caaaatgccc tcgaggccga ttcgtatttc
    13561 atcggtgttg tggaagggga agaaatacgg cttgaactgc tttacgatgg cggggaatac
    13621 tttcacaatg taaaactacc cagaaggggt actttttcaa gctgggttat cgataatcag
    13681 cgcgaactct tcctgcccga cctgcgccag aaaatcgtac tggaaggcgt agatgttgtt
    13741 ctcgttggcc agaatcaaac atcgctgtcc tgggtgggcg tgcccatgcg cggcacacat
    13801 gttgatggca tcatggccat tgcctcttat caacccaacg ccttcgaccg cggcgatgtg
    13861 gaactgctct tcaccattgc ccaacgcgcc gccctcgcac tggataacgc ccaccatcac
    13921 gccattgccg aagaacaggc acggctcgac agtctcaccc acgcctacaa ccacggatac
    13981 ttccttaaac ttctaaacca acaagctcag gaatgtctcg acaatcagca gccactcagc
    14041 ctcatcatgc tcgacattga tttcttcaaa caatataacg acacctacgg ccacatcgtc
    14101 ggcgacgaag tcctcgtcag cctgtgcaga accatccaaa tgcacatcaa aagcacagac
    14161 gcggtcgggc gctggggcgg agaggaattc gtcattttcc ttccaaatgc cgatggaata
    14221 caagctacac agatcgcaca acgcatccgt gaaagccttg ccgcgcacaa aatccaaaac
    14281 aacgaacacg ccaatcttcc cattccaacc atcagcatgg gcattgccgt cttcccaaca
    14341 gaaaccgacc aaaccacaaa actgatcgac ctcgccgaca accgcctcta caaagcaaaa
    14401 gaacgcgggc gggatcagat cgaacccaca gccgcgttct gggaagaact caataaaagc
    14461 agttaggcta cttcaaaacc gcgcctaaag cctgcaaggt tttctccaca cccgcatcat
    14521 ccacccaata atgcgtcacc aaccgaaacc gacgggggcc agcccaatcc accaacacac
    14581 cgtgtttctt catttcttca acgacctgat taacacttaa tttgacctca tcagccaggt
    14641 caaaataaac catatttgtg gatgaagggc cgaacgcatc cagctttaat ccccgaacct
    14701 gctgcaaccc ctcaaacagc ttcttcgccc gggcatggtc ctgcccaagc cgcgtagaca
    14761 ttttctccaa cgagatcatc cccgccgccg ccaacacacc tgcctgccgc atccccccac
    14821 ccagcatctt gcgaagatga cgtgcacggt taatgaactt cctccccccc accagcatgg
    14881 accccacagg cgcaaccaat cctttgctca aacaaaacat cacagaatcg gcaggcgcaa
    14941 ccagatcctt cacggagata ttcaatgccg ccgccgcgtt aaaaatccgt gcaccatcaa
    15001 tatggaaagc caacccgttt tcctgcgcga tctttccaac cgcctgcaga tactccgcgg
    15061 ataacacaac cccgccgcac acattttgcg tattctcaag gcagatcagt cgcgtaatgg
    15121 tgtgatgcac atcctcggtt tggatggatg cgcgaatctc atccagcaga agcgtgccat
    15181 cggcttgatt ctgcaaaggc cgtggatgaa tcccgcccag tgccgacatt ccacccgcct
    15241 cattcaaata gatatgcgat ttatcaccaa cgatcacctc atccccacgt tgacaatgca
    15301 ccagcaaagc cagcaggttc cccatcgttc ccgaagggac aaacaacgcg tcttccttgc
    15361 caagcatttc ggcggctttt tcctgcaacg cgtttacggt tgggtcatcc atgtaaacat
    15421 catcacccac ttccgcctcg gccatcgcct cacgcatctc aggtgtaggt tttgtgacag
    15481 tatcagaacg aagatccaca aattccatga caaactcctt taaatagact tgctgcctct
    15541 tccccaaaaa cggaaacagt ccacgttcat tccatgaatt gatttcctca accccgcaaa
    15601 tcggtcaaca caccttccaa ctctttgggc aaagcagcag taaacaccct tggttctttt
    15661 tctccgggca aaacgatctt caaacgatag gcatgcagaa aatgcctgtc aaggtcaaca
    15721 gtcggattct tgcgcccata aatggaatca cccacaatcg ggcaccccaa aaactgacag
    15781 tgcaaacgaa tctgatgcgt acgccctgtg tgcggatgaa actccaaaag cgtatgattc
    15841 ttgaacgctt caagcgtttt gtattcactg accgcttcac gtcccttgcc cgcagtaaca
    15901 atggccatct tctttctgtg actcgggtcg cggccaatcg aagcctccac acgcccggtg
    15961 ggagtgggcg gcataccatc caccaatgca agataggtct tttccaccgt ccgcaaacgg
    16021 aattggtctt gtaaccagcg gtgtgcgcgc tcgttcttcg ccagcacgat cagacccgac
    16081 gtttccttat ccagccgatg caccagaccg ggacgttcct cgccgccgat gccttcgagg
    16141 tcggggtcat atcccaacac cgcatgaacc aaagtcccag aatcatgccc ggcagcagga
    16201 tgaacgacca tgcccgcagg cttgttcaca atcaacagat catcgttctc aaaaataata
    16261 tcaaggggaa tatcctcccc caccaaacca cttggcacag gcggcggaat acgcacttcg
    16321 atctctgcgc ccggatcaat ggattgcccc gatttcttcg cgcggacatt gttgatcgtg
    16381 acaaaacctt catcgatcaa tccctgcaac cgcgccctcg aaaactcagg caggcaagtc
    16441 accagaaatt tatcaaggcg atctgctttt tctccgtcat atacgaattt ttcaacacgg
    16501 tcactcacga ttgcccgcca ttcgatttgg gttgttcacc gccagccact ccatccacgg
    16561 ggccaacgcc attcaaaact gcttctgcag gctgctcccc ctctggggtg gattgttgcg
    16621 cggcattgag tttttctttg cgctcattca gccacacccc cagtaaaagc acagccacac
    16681 ccaccgaaat ggatgcatct gcaatattga aaacaggaaa ggtgccgaca gaaataaaat
    16741 ccgtcacctg cccaacgcgc agacggtcga tcaaattccc cgccgcaccg ccaagctgca
    16801 accccatcgc caaacgcaaa gtccaatcac ctgcctcaac ccgcggataa taataaatga
    16861 tcgcgccgat cacaataaaa gccagaatgg tgaacacgat gttcccattt tgaaacatac
    16921 caaaggcggc ccccttgtta taccaatgca caatgcgggc atatgggctc aaccacatca
    16981 aagagtcggg cagccactgc atgccaaatt caatgttcgt tcgtacaagc cacttcgtcc
    17041 actgatccaa ggcgatgctt gtgcccccca cgccaaataa aaccagataa tccttcacaa
    17101 cacgattcaa agttcacctc aatttcaaat ttgtgcgcct attgtaccgc acgggtaaat
    17161 acacgcggca cacgcgcact gatgttggtc attacttcat attcattcgt acccgtccaa
    17221 tcggccaggt cttcggctgt gatgccgccg ccgagcaaaa tcacctcatc cccaaccttc
    17281 acatcatcct cccctgcatt gaccataaac tgatccatgc agatgcgccc cacttgcgga
    17341 tatagcctgc cgttgatatt cacccgcgct tgattcgtca tgcggcggaa gtatccatcg
    17401 gcatacccac acgggacagt aacgactcgt gactccgctt cggcctgcca caacgaccca
    17461 tagctgactc cacgcccggg ctgggtgatc ttgctgtaaa cgaccttgga gcgccaggtc
    17521 agtgcggggc ggacctcaat ggtcttttcc acatcacggc cagggtatac accataaaac
    17581 aagacaccgg gccgcaccat gtcgtaatag ctttcagggt gcaacaggat cgcacccgaa
    17641 ttcgcagtgt gccgaatgcg tggctgcggc gcccccctct tctcaaacaa agcaaggatt
    17701 tcttcgaagc gttcaagctg caaggaaagc ggggtcatat ttttctgcaa cgacatcact
    17761 tctgagtttg caaaatgagt aaagatgcct tccaaatcca agtggctaca cgacaaagcc
    17821 agttcaatta aagagtccgc ttcgtattcg cggacaccga tgcgttccat gccagtatcc
    17881 actttcaagt ggatcttgag tcgcttacgg gtggaggctg ccaattgttc ggcagcagtt
    17941 aacaggtcca atgaagaagc ggcgagggtg aggtcgtatt caaaaaaatc aggcagctgc
    18001 tgcgggagag tcccacccat cacgaggatg ggagtcgcaa ttcccatttt gcgaagatgg
    18061 atgccttctt cggcaatggc cacaccaatg taatcggcaa agggggcaat gaagggagcc
    18121 acgccatcaa ctccgtggcc ataggcattg gccttgagca ccacgaggac tttggcaggc
    18181 gctacctttg cgcgaatgtt ttcaagattt tgttttagtt gggtgaggtt gacttcaaga
    18241 aatgtaggac gcattttttt attttatcgc accttcacct ttacttcgcc cgccagtgat
    18301 actccacatc gggttcggat tcgggtggag ggctggagcc aaatccctcg gcgatgaagt
    18361 catttttttc ataaaatgcc cgcgccattc ggttggcggt gtgggtgtat aaccagatgt
    18421 gtttgggcaa aagttcgcgg gctttattga gcaatgcctg cccaatgccc tgacggtgcc
    18481 aggcagggtg aacatacagg cggtcgatga gttcgccttg aatggcgaga aaacccaggg
    18541 gttcgccatc gcgttcatat acccagatct ggttttcatt gacaacgata ttgcggaaat
    18601 attcctgtgc ctcgctaaat tcatatccca tacgggccat catttcgggc atggcaacgg
    18661 tttgggcatc gaaccatagg cgggtaacgg cttcaaaatc ttctgttttg tagggtcgga
    18721 tggtgtttgg catgggtgga gtataaccga acgggtcagg aaatcagtgg caaggaattg
    18781 atgaccttac gctattaaat tcgggacgca gatgaacgct gaaaacgctg attttgggag
    18841 gtcttcggca gaatcctcaa aaaaatccgc gcccaagaac aaagtgcaat gtgatcatag
    18901 cggggaaata aaaaactcat ccacgaaaca ggttcgggat gagtcggtat ggggttgtac
    18961 aggtctgatc aatgccatgc gccgaggatg gcacccatca caacaaaagt gacgagatgg
    19021 ttccccactt cgatcatcca ggcttttatg cggtctgcaa ataatttatt cgtcaggctg
    19081 gagggggcta caaaaccgag ccaaagcaaa aagcctgtca atgcgccaga gccaagtgtg
    19141 gcgccgcccg acaggcttcc catggcattg accatgagcg ccataaacat cgcctgcaca
    19201 aatgaagaga ccacaatcag tccccagaca atggccatgc ttgtgccgct tcctggcgct
    19261 tgaacatctg ctttgcctac agccttccac cagatcggga aaaaggtctt gggaccaaac
    19321 cacagaccac cgatcatcat acttgccaaa acacaaacca ccactgccaa ccagtttata
    19381 gaactaaaat ccatctcagc ctccgttgat aaatttgatc agccgcgcca atcttttagt
    19441 cattataaca gaaaatttgt tctaattcac agttgtgctc tcgtctcgat acggatgcac
    19501 aggcgaaaaa aagcaaaacg agcaggttca tcacctgctc gttgttcgtt tatcagttgc
    19561 tgttctactg taaaaactat ccacccaagg cttcattaag ccaatccaaa aatccttttt
    19621 cctgcggttt gaccgaagta cccagactct cagccagctt ttcaaacaat tgatgctgtt
    19681 cctttgtcag tttggtgggg atcgccacgt tgacaatgac cagttggttg ccgcgatctt
    19741 tgcgccgcag atacggaaca cccttcccct tcatggtgat gaccttgcca ggttgggtac
    19801 cagcaggtat tttcagtttc tcttcgccat cgagggtggg cacatccacc tcggcaccaa
    19861 ggacagcctg tgccacattg atatcgaggt tcaagaccac atcattatca cggcgtttga
    19921 agaattgatg ctgctttaca tccagcacca ggaacaggct gccgctgggc ccgccataca
    19981 caccgggagc gccttcgccg ttcaggcgaa tctgtgtgcc atggtccacc ccggcgggaa
    20041 tctgcacttt ctttttgatg tgcttgcgtt ccagaccacc accccgacag gttttgcatg
    20101 gcgaggagat cgtttcaccg cgaccattgc aggtggggca ggctgctgtc tgcaccattt
    20161 gacccagaaa ggtctggcgc acctgacgga cttcaccctg cccgttacag gtggagcatt
    20221 tcactggcga tgtgcctggc tcggcaccat tgccattaca acgtgaacag gtttcttcgc
    20281 gctcgaattc gatatctttg tccacgccaa agaccgcttc ttcgaaagtc aacgaaacgg
    20341 ccatttgcag gtcacggcca cggcgcgggc gtgtgctgtt gcgtgaacga cggccagagg
    20401 agaagccgaa tccgctcagc aggtcttcga agatatcgcc gaaatcggcg ctgtaatcgt
    20461 ggaagccacc gctgcccatg ccgccaagac cttccttgcc aaaccggtca tagcgcgcac
    20521 gcttgtccga atccgaaagc acaccgtagg cttcgttgat ttccttgaac ttttcctcgg
    20581 cgtcgggttc cttgttcaca tcggggtgat attggcgcgc gagtttacga aacgcggctt
    20641 tgacttcatc atcgctcgcg ttccgtccca cacccagagt ttcgtaataa tcctgattgc
    20701 tcataattta agttacaggt tgcgggttac aacttgcagg ttacaaacct tcaacctgtc
    20761 acattgaacc ttaaaccgac ctactccttg acttcaccat ccaccacgtc agggccagcg
    20821 gatgaacttt cacccgcttc accgccaccc gcggcctgtc cctgctcata cacagaggca
    20881 ccgatctttt ggatggacag ccccaaagcc tcagttgcgg ctttgatctt ttccaaatct
    20941 tcaccctgtg cggcagattt gacctctgca gctttggctt ccacgtcagc acgcacatcg
    21001 gcgggaattt tatccccgaa ttcacgcaga gctttttcgg cagcataagc agtgttgtcg
    21061 gcgttgttgc gggcttcgat caagtccttg cgtttgcggt cttcttcggc atgggcttcg
    21121 gcatccttgc gcatcttttc cacttcgcta tcgctcaagc ctgaggatgc agtaatggta
    21181 atgtgctggg aacgacccgt ggctttatct ttcgcgctga ccttcaaaat gccgttcgca
    21241 tccacatcaa aggtcacttc gatctgcggc acaccgcgcg gcgcaggcgg aattccatcc
    21301 aggatgaatt tgcccaggga tttgttatcg ttcgccatgg ggcgttcacc ctgcaaaaca
    21361 tggatctcca cctgggtctg gttatcgcct gccgtggaaa atacctgtgt gcggcgggtt
    21421 ggaatggtgg tgttacgctc gatctgtgga gtggcaactc cacccaaggt ttcaatggaa
    21481 agagtcaacg gagttacgtc aagaaggaga atgtccttca cttccccacc gagcacacca
    21541 gcctgaatcg cagcgccaat ggcaaccact tcatcgggat tcacgccttt atgggggtct
    21601 ttgccgaaaa aggcacgtac acggtcttga atggcgggca tacgggtcat gccgcccacc
    21661 agcaccactt cattgatgtc acctgcgttt aatccagcgt cagataatgc ctgtttgaca
    21721 ggagcaatcg ttcggtcgac aagatccgcg gtgatctgct ccagtttggc gcgggagaga
    21781 gtcatcacca aatgtttggg gccattcgca tcggcggtca gatacggcag gttgatttcg
    21841 gtctgcatca cagtggagag ttcgatcttg gctttttcag aagcctcctt caaacgctgc
    21901 aaggcctgac gatcgttgtt caaatcaatt ccgttatctt tcttgaactg ttcgatcaaa
    21961 tagtccatga tgcgaagatc gaaatcatcg ccaccaagga aggtatcgcc gcttgtagca
    22021 cggacctgga aaacgccatc gcccacatcc aaaatggaaa catcgaatgt accgccgccg
    22081 agatcataca cgacgataac ttcgttcttc tttttatcaa ggccgtaagc cagtgaagaa
    22141 gcggtcggct cgttgatgat gcgtaacacc tcaaggccag cgatcttgcc cgcatctttt
    22201 gtggcattgc gctgggcgtc attgaagtac gcagggacgg taatgaccgc ctgtgtgatg
    22261 gtctcaccaa gataggcttc ggcatcagcc ttgatcttcg cgaggatcat cgcagaaacc
    22321 tcaggcgcag agtaatcgcg ttcgccgagg cgcacacgca catcgccgtt atcagccgcg
    22381 ttcaccgcgt aaggcacacg tttgcgggtg cgctccactt cagggtcgtc agatttacgg
    22441 cccataaaac gcttcactga aaaaatcgta ttctgcggat tggtgatcgc ctgattgcga
    22501 gcgacacgcc cgaccaaacg ctcactgttc ttattcatcg ccaccaccga agggacaagc
    22561 cgctctcctt cagcagatgg aatcacaatc ggctcaccgc ccgacataac agcggccacc
    22621 gagttggtcg taccaagatc aataccaatg atttttgcca tgatttctcc taagtttcaa
    22681 gttgaaggtt gaaagccgaa ggttcgtaca aacttgcaac ctgtaacctt caaccttaac
    22741 cgaatttact gtgccactct aaccaatgca gggcgaatta cccgatcacc aagcatgtat
    22801 ccgttttgca ctacatcgat cacatgctcg ctctccacac catcggcggg ttcgtgcgag
    22861 atcgcttcat gaaaattcgg gtcgaagggt tttcccttgg catcaatgcg cttcaggcct
    22921 tccccttcca aaatattctg catcttgcgt gcgatcaact caatgccgct tgcccatgca
    22981 tcatccgcag ggcggttttg caaggcgcgc tccaaatcat cgagcgcggg caacaccttc
    23041 ttgatgatgt cacctttcat actggcatag gtcaattcgt tatcacgctc aatgcgcttc
    23101 ttgtaattct ggaattccgc ctgagacctt gcccacccat ctttatattc gatgaccctg
    23161 gcttcggctt ccttcaattg attgatcaac gcttcgcgct ccgctgaggc ctgcccattc
    23221 tcaacgggca tttcttcggc tgagggttga gtctcatcaa tttcttcctg tttatctttc
    23281 tcgtctgcca tgtttctcac tctcaaattg gtaattcgta attggcaatt tcatccacaa
    23341 acgaccaatt acgttttttt actcaccaga aatcgtatcg ttcaccagat cacttagcag
    23401 gccagccaca taacgcacag tggggatcgt ccgcgcatag gacatgcgca tggggcccaa
    23461 cacaccgagc gtacccgtca acaacccggg gacaccgtat ctggcgacca ccatcgagca
    23521 ttgacgcaat tcatcccagc cgccggagcc gccgatcaat acctgcaacc caccgacgct
    23581 gctattggaa gatgcacgcg ccagcaaccc ctgcaaggtg gatcgttctt caaaaagacg
    23641 caaagcccaa cgagcctcgt ccgattctgc gaattcgggt tcatccaaca cattggtgag
    23701 gccgtcggta taaatttcac ctgaaacgct ttcagaggtg cgtttcatat cctgtaacag
    23761 gatggtgatg atatcctgtt cgagtgcgtc agcgcgggat gaaaccgcag cgatggagtc
    23821 cattgaaaga ccggccagca gcgaattgag atgagccgcc gcctgactga gtctttcctg
    23881 tgtgacgatt tcagccaatg tcaaaatctg ctggttgact tcgccgccat ccatgaccaa
    23941 gaccatcaag acctgacgtc cctgggtgga gatcaactca atatgcttga aacggatggt
    24001 ctctgcatgg ggtgcggtga ccaccgaaac gccctgcgat tgattcgcca ggatcgaagc
    24061 ggcaagggtc atccactggt ccatatcggg gcgggcttga aagaactgat gcgaaatggt
    24121 gttttgcacc gaagcgggca attccgcttt gtggatcatc tcaccgacaa aagcccgata
    24181 gccatcttcc gagggtacac gacccgctga ggtgtgaggc tgtcttaggt agcccatctc
    24241 tgtcagcgct gacatttcat ttcggacggt ggcagggctc aggtcaagat ggaagcgctc
    24301 caccaatcgc tttgagccga cgggttgggc cgtctcaata tagtcgcgaa tgaggagctg
    24361 taagatggtc cgctgtcttt cggtaagttc tggcatcgga atgcaagttt acatgtggaa
    24421 atggagggcg tcaataagag gagtgtaaga gtttataaga aaaaagagaa agaagaaaga
    24481 ctggcatctt tcttctttct ctttctcttt tgttgtatca gggtgaaatt cagggtgtgg
    24541 agatgggtct ccaggcgggg tcgaaatcgt caaaggtgac atcttcggtg acacgggtga
    24601 ggttgccgcc tgccggggtg gagtaataga cgtgatatcc gcggtcgccg ccgctttcgt
    24661 aggcgatcca aaaactgccg ggagaataat ccacatcttc caccgagcgg acactcagtg
    24721 taagtttgag ggcggcgtca ttgttatcgc caattttatg tgagaacaaa ttggggaagg
    24781 tcgcttcttc agggcggcgc tggctgaaga gaatgaattt tccgtcaggc gtccacacag
    24841 ggtattgatt cgagaatacg ttgccgcttg tgaaaagtag tctttcattg gcgccgaggc
    24901 tggacatcag ccaaagctca taagtggcaa cacgcttcaa agagaaaaca agctgtctgc
    24961 catcgggaga ccaggcgggc tggcgcgcag cggtggtggc atctgtttca acgaggcgtt
    25021 gaatcttgtc gttttcaata tcataggagt agatctgcat atatccatcg cgcagggaag
    25081 taaaagcgat ggattttcca tcgggagacc aggcgggctc aaaatctcca ccgagggctg
    25141 tcggcagggg ctgcaagcct gtgccatccg agttgatgat gaataaactc gcctcaggat
    25201 aggtatcctt ttttacagcg cagggggaaa tgaaaaccaa tttgcttcca tctggagccc
    25261 atgctggctg acatgcaccg cccgtcatat tggtgaccgc aatggattga tcagtaagaa
    25321 tattggtgag atagatctgg ggcaacccgg tacgggtgga aacatatgcg atctgcaggt
    25381 aatctgcacc gatgggggta ggttcaacgg cttgattcac agatgaggtg ggtgttacct
    25441 gaaccggggt ggacactacg agtgtggcag tcggttcatc caccacaact gcgacagggg
    25501 ttggaggtac agatgcaatt tctgttggga tggttgtggg ttcggtggtc ggcaaaaatg
    25561 ggaacaatct ccgcaccgat tccggcatgg catttggaac aaaaagcatg atgcctaaaa
    25621 atacaacaaa acccaccgca agcaagccac ccacgagggc acgcatgttg cgacggttac
    25681 gttcgagtaa ttttttccgc ggggagacaa attcctgatc ttccagagca gcggaagagt
    25741 gtttaatgat attgggggcc ttaacgggat cgctttttct ttccgcttcg gaaggagggg
    25801 gggcaatcgt ataagttcca agcaactgtt gggtcctgga tttggcattg agcatggcca
    25861 gtttgaattc ttctgctgtt tggaaacgat cagcaggatc cacttccatg gctttttcaa
    25921 tggcagccgc aaggcggcgg gcaacgtctg gattgcgctt gcgcagcggt gtaagttgag
    25981 cgttatccat ggcgcgggca aggccatctt ccgggatgac accggtcagg gccgcatata
    26041 gcgttgcacc aagggagtag atatctgtcc gcgcatcggt gcgggcggtg ccatattgtt
    26101 caggaggaga atacccgggc gtcattgcgc gcgcgccggt ggtggtggcc tgattcccct
    26161 gcatgatctt ggcgagacca aaatctacaa ggaaaatgtg tccatcaggt gagatcttta
    26221 cgttaccggg ttttatatca cgatgcagaa tagggggttt acgggcatgt agatatgtaa
    26281 gcgcatcaca aatagccgca ccgaccagaa taacctcatc ttctggaaga gcccccaacc
    26341 gttccatgcg ttggcgtaaa tcctccccct caatgaaatc catgacgagg tattgacctt
    26401 ccccggcaat gacgaaatga tctgatacgc ggggcagatt tggatgacga atatttgcaa
    26461 gaatgaccgc ctcaaggcgg aattgacgtg catattcctc tgttgtaaac aggttctctt
    26521 taacggcaac atccacgctg agattttcat ccacagcgcg gtacactgac cccatccccc
    26581 cctgcccaag attctctaga atccgatagc ggttatgtaa aagagtttcg ttttcgagaa
    26641 tcatcaaatt cctgtctgca ttttttcatt tcataattcg aaaggtattc tactctcaag
    26701 aaggaaagtt ttcatttctg ttctatttca atactatttt atttcaattc aaaaaattgc
    26761 ttattaatat ttttgaaagg tattgtagca aataaagatt aaaaacctgc cacacatgag
    26821 atgactcaat aaaatcgaag cgccagagta cggtattata ctctggcgct tggggattct
    26881 aggtgaaagt acttattttg gagttggtcg ccaaacggga tcaaaatcca agcctggatc
    26941 tgtggtgatg cggacgcgat ttgcgccggt aaccgtcaca tagatgatgt cattattctc
    27001 accagcttct tcaccttcaa acagcaagtg aaaaccatcc ggggaataat gaacatcttc
    27061 cactgaagtt acacccaatt ggagcaaggt cccctgctcc acggagcgat ctgtggagga
    27121 aatgctcatc aaatacggca gacagaacga agtggcacaa cgctggttga acaggatcaa
    27181 acttccatcc ggtgaccagg taggcaggta atctgaaaac gccacaccgc tgcgaacaat
    27241 ctgttttggc tcggtaccat ctgcattcat caaccagatc tggtaaacac caaagcgttt
    27301 gaccgagtag acgatctgct tgccatccgg tgaccaggaa ggctggcgcg actcaacact
    27361 cttggcgccc ttcgtcactt caatggcatt cgagagatca tccaacttga ccatgaagat
    27421 ctccatctgt ccggtgcgca gagacgtgaa caaaatggat ttgccatccg gcgaccaggc
    27481 tgggtcaaaa tctccaccgg ggacggtggc aatcggggtc aaatcagtgc catctacatt
    27541 gataatgtac agggcggctt gatagtagat ttcatccatg cctttacagg gagaaatgaa
    27601 gacaagcctc tgtccatcgg gcgaccacga aggctggcag gcaccatcag gcatctcagt
    27661 gatctgggtt gcggtctggc tggcgatatc tgccaggtaa atttgcggca aaccggtacg
    27721 gttcgaagca taagcgatct gccctgaacc gccgccgatc ggcgtaaccg acagagaagg
    27781 tgtcggcgaa ggctgaacaa gagtgggggt cgaagtaacc gaaggcgtat tcgtcaaagt
    27841 agcttcaaca ggaatgttcc cggttggcgg tggagtattg gtatgagttg gcggaacctt
    27901 ggttttggtt gcggtaggag ggacatcggt cgcagtggat gtggatgtca gcgtaaaggt
    27961 tggcagcgca gttgaggttg gcgaagaagt ggctgtactc gtcggcgcaa ggaaaggtat
    28021 ttgagcacga agatctgcag ggataacacc cggggcaaat acgagaacac cgatcacagc
    28081 caataaaatc aaaacagcca taaaacgcat caacgcattt ctacgtttgc gctcacgttc
    28141 tttttgtttc ttaagcggtg agacaaaagg ctgctcttcc tctgcagatg caggctgcac
    28201 ggggccggtc atttggcgcg gcggagtctt ttccttttct ttggctgcta caacttcact
    28261 gggatcgttc ggcaaaaacg cctcggcagg cggtggcgta actgtgtaac cacccggccg
    28321 cgactgcgat tttgaactcg aacccaacaa ggccttcttg aaatcctctg cgttttgaaa
    28381 acggtcgctt ggatcaatag ccatggcttt ttcgatggca gcagcaaagc ggcgcgaaat
    28441 cttcgggtta cgtttccgca gcggagtcaa ttgcgcgtta tccattgcac gcgcaagccc
    28501 atcttcaggg atgacaccac acaaggcggc atacaaggtt gcgcccaatg aatagtaatc
    28561 ggtgcgtgga tctgtgcgtg ccgtgccgta ttgttcggga ggagaatatc cgggcgtcat
    28621 cgcgcgggcg ccagtagtgg tagcctgact ggccccctga tataccttcg cgaggccgaa
    28681 atctacaaga aaaatatgcc catccggggt gatctttaca ttaccaggct tgatatcacg
    28741 gtgaaggatg ggcggcttgc ggctgtgcag ataagccaac gcatcgcaca tcgccgcgcc
    28801 aatctgaacc gcatcttctt cagtgatggt accaaggcgt tccatgcgga aacgcaggtc
    28861 ttcgccttca ataaaatcca tcaccaaata ctgaccctgc tcgcccaatt caaaatgatc
    28921 ggaaacacgc ggcaggttgg gatggcgcaa attcgccaga atcaccgcct caaggcggaa
    28981 ctggcgggta tattcctcag tggtgaacag gttttctttt aaagcaactg ccacgccaag
    29041 attctcatca atggcgcggt aaaccgaccc catgccgccc tggccaagaa tttcaatgat
    29101 acgataacga ttatgtagga gggtgttttt ctctagcgtc atctacgaac ccttatggat
    29161 ttatacggaa tggcgcgatt atatcagatg gaattctcgc ccgtattcca tgaggccagc
    29221 tttaacctta caaataacca cggaccgccg cctgccaccc accagccgat cagcgtgtaa
    29281 cggataaacc gcagggtgta aaaaatgaat ccctcgccgc gcgggaaaat cgcccccaaa
    29341 cccatccaca ggatgaggat tccgataagg ccgatgacat aacgaatggc gcgcttcgcc
    29401 accggacctg aagcctgata cccgccaaag gagtgaatcc acgccgcgcc aatgaccacg
    29461 ccaaaaagcg tccctgctga ggtgaaggta ctgttcgggt cgagcggagc aggcacctcg
    29521 gtcccggcca gagccgcgtt tgcgatccag gtttcaggaa cctggaatcc gcttcgaaaa
    29581 agcgaagcgc caaatccgat ggcgatgaaa atcaacgaaa cgataaaacc gaccatgatc
    29641 tgcccgccca ttgatttttt ctccagccag gccgctgccg aatcccaaaa gcggacaaag
    29701 gcccacagga tcgccgcacc gatcagccag cccagcaaca catcatgcag gaaatgcgcg
    29761 ccgagaaata aacgtgaaaa accgatcagg aaaataaccg tgatcgaacc aacccatacc
    29821 caattctttt tactgtacgc cgcaaacatc ccccacacac tcgctgcgat ctgcgcatgc
    29881 ccggagggga tgccaaatga aatttcattc cacaatgcgc ggacatcgga acttacccaa
    29941 taggggcgcg gcccggcgaa caataattta cccacatgat taaataaatt gcttgttacc
    30001 aaaatgaaag ccaccctcag acccaacgca gagtcaacgc tccaatataa aagaggcaga
    30061 actagcaaaa agaattcctc gttgccgagg tagctgaaaa atttcatggg agcaattaac
    30121 caatccccca ggctttgaat cgcgataata agggcgatac ctccctcaat taaggtctgc
    30181 ataccatcct ccagtagggt tttttggggt tgtacaattg acgaacgggc ttcttgtatt
    30241 gtacactcaa accatggaaa aatcatccat caccaactcc aacgctgagt catctgcaag
    30301 aaccagtgta tgggattggc gcgccattct gttgacgatc gccttgatcg aaatttcaag
    30361 ctcgcggctg gtcatcactg aatgggttcc ctatctctat ttcacgcaaa ccatcagcct
    30421 ggcgggcgtc attctcggtt ttacgctggg ctacagcatt ttttcacgaa aagcagccac
    30481 acgcattgcg gtgggatatt cgctcattct gctccctgca caattattaa atgccgttga
    30541 aagaaccgac tgggtttggc ctgatctggt cgtcctgttt aaccgtctgt tcacttcgat
    30601 ggcgcaattc atcaaaggcc agcccgtata tgaccagctg ttctttattt cgctcgtgac
    30661 agcaggctat tggatcatcg gcatttcggc gggctactgg ctcacccgtc atcgcgattt
    30721 tctaaacgtc atcctgcctt catgtctggc gatgttggtg gtgcagatct tcgacccgac
    30781 cgaatcgcgg cgcatgtggg cattggcctt ctatttattt gcggcattgc tcctgcttgg
    30841 gcgcttgtat ttccttgaaa atcaaaccta ttggaggaag acccgcttcc tgctcacggc
    30901 tgaaaccatc aacaacctgg agcgcggcgc gctgaccgtg gcggcgatca cggtattcgt
    30961 agcctggtcc atcccgggtt ggatcaagca aattaaacct gcagcgaagg cctggcgcga
    31021 aatttccgag ccgatcctgg aaaggctttc cgatgcggtc agcgccctgg aatcaccgta
    31081 tgcgggcagt aactcaggcg gggaatttta cagcagcgaa ctttcgctgg gccaacaggc
    31141 cgcagaaggt gacacgcccg ttttcttcgt cacagtgaag gaaagtgaat ttgtgccgat
    31201 ccgcaattat tggaaaggca gaatgtacga cctgtacatc aatggccgct ggacgacact
    31261 ttcagattca aatacgcctt tcgtgccaga taccgatcaa ttggaagttg aatatccaga
    31321 gaaccgcttt gaaatggaat tcaccttcac caacaatttg acaagccaga acctgctgta
    31381 tggcccatcc gaaacgatct gggtcagccg cgaagggacg gtaaccgcaa ccgagatcgg
    31441 ctcttcatta aaggatgtca ctgcctggaa cgccaccccg ggtgtttcga gcggaggcag
    31501 gtacacggtc cgcgcgttga tcgcagaccc cactcaggaa gacttgcgcg ccgccggcac
    31561 tgactatcca gagtgggtta ccgagcgata tctgcaagtc ccggaaaaca ttgccccagc
    31621 cctgcgcaca ttggcagaag agatcaccgc gccctacgac aatccatttg ataaaacaca
    31681 ggcgatcacg gcgtatttac gcaacgagat cgagtacacc accgaaatca ccgccgagcc
    31741 gcctgaaaac agggaccccg tgttgtgggt gcttttcgat tacaaaaaag gtttctgcat
    31801 gtactatgcc agcgcagaag ccttgatgct gcgttccatt ggcattcccg cccgcatggc
    31861 agtgggcttt gccgaaggca cctttaatga aattgacgag cgttatgtcg tcacttttga
    31921 ggatgcgcat gcatggcctg aggtatattt ccctggcatt ggctgggtgg aatttgaacc
    31981 cacaggcaac caattaccca tcgaacgtgc cgcgaccaga gatgtcatca accccgatgc
    32041 cacccccgac cctgtaaccg ccgaaacacc cgccgccaat ccgttggttc ctccgctgtt
    32101 ggaagaacca cccaacctga ccacccagca aaacagcgga gccacccaag ccgaccgaat
    32161 tgaactttac aagaccatcg ccctgtattc actgctgaca atgacggttg tctttgttgt
    32221 ttttgccttc cgccgctatt cattggatga ccgactgcct gtgtaccttg caagccgcta
    32281 cacaaaaagc ggaaatcaac cgccgcgctg gctcaatcgc tggctgcgtt ggaccaccct
    32341 ctcccccatt gagcgcacat ttcaggcagt caacctcagc ctgtacctgc ttgggcggcc
    32401 gcaaccccgt catatcactt cgcaggaacg ggccaaagca ttgatcgaac gcatgccgtc
    32461 ggcagagccg caagtgctgg ccttattaca ggaataccaa tccaccatat acacgcccaa
    32521 aatgggaagc ctcacaaccg cccgaaaagc cgccgccctt atcctactta agacatggca
    32581 gattcgtgca aaagaagcct tgcaatcctt cgattaccgt tataatcaac tacggtaaac
    32641 gcctatgaat gaacagaccc gtcctacaac cgttccagac cagttgcaga ccttgagcct
    32701 gcaggtgggg aagattcgca ttgacctgga agaaagccga ctcaaattgc ccaagaacac
    32761 gggcgaagac ctgcgtcaca tcgaaacctc gctgatgctc atcagcgaaa agatcgatgc
    32821 cttccaacgc gaacacaaca acatgctggc gcttgccaac atcggtcagg tggtcaattc
    32881 atcactcgaa ctcgacgaag tgctgcgcat cgtgatggac aacatcgtgc gcctcaccaa
    32941 agccgaacgc ggctttctca tgctgcgcga tgacaaaggc gaaatggtca cccgcatggc
    33001 ccgcaactgg gaaatggaat cgatccactc cagcgagttg accgtgagcc gctcagtggt
    33061 agggcgtgtg atcgaaacag gcgaacccat catcaccacc aacgcacagg aagaccagcg
    33121 ctttgtaggg caggaaagca tcgtagcctt caacctgcgc tccattttgt gcgtacccct
    33181 caaagtaaaa aacgacctca tcggcgtcat ttatgccgat aaccgcatcc gcgcgggcat
    33241 cttcgctgat tcagagcgga acctgctggt tgcttttgcg aatcaggccg cagttgcgat
    33301 cgagaacgcc cgtctgttct cctcgctcaa gaacaccctc gaagaggtca caggcttgaa
    33361 gaatctgatg gataacatct tcgcatccat cgccagcggt gtgatcactg ccgatattca
    33421 gaatcagatc acgctcacca accgcgccgc ggaacgcatc ctcggatctt cctccagcga
    33481 catcatcggt catcatctta acgagacgct tgcttctgtt tcctccgaac ttgagccgca
    33541 tctgacagag gtccgccaaa ccgacaaacc catcgtagac cttgagatca gccaccacct
    33601 gcccaaccac ggcaacgtgg actggcgttt caatctctct ccgctcaaag atgcaggcca
    33661 gaagacacag ggcatcgcca tcgtgcttga tgacctgacc gagcgcaaaa agctcgaagc
    33721 ccagcgccga ctcttcgagc gtatggtctc ccctgctgtg atcgaacaac tcgacccgaa
    33781 cagtttgcag ctgggcggta aacgcacgaa catcaccacc ctgttcgcag atatccgcgg
    33841 attcacctcc tttagcgaaa atcaggaacc agaaaaactg gtgcagatct tgaaccgcta
    33901 cctcgccgcc atggccgaag ccgtgctcgt gcaggcaggc accattgaca agttcatggg
    33961 cgatgccatc atggcctggt tcaacgcccc cattccgcag cctgaccata ccctgcgcgc
    34021 cgtgaagacc gcgctcatca tccgtgaatc ggtggaaaac ctgtataaag aattgccgcc
    34081 tgaattccat ctcggttttg gcgcaggcat tcactgcggc gacgccgttc tcggtttgat
    34141 cggcaccgaa aagagactcg aatacaccgc catcagcgac gccgtcaaca ccgccaagcg
    34201 cattcaggaa aactccgcac ggaatcagat cctcatcagc gcctcggcat acgaacgggt
    34261 cagcaaggaa atcaacgcca gaccccatgc cgatatgagc gtgaaaggca aaacccagcc
    34321 gctgggagtg tttgaggtgc tggggttgaa gtaggggaat agnnnnnnnn nnnnnnnnnn
    34381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34441 nnnnnnnnnn nnnnnnnnnn nngcaaaacc cagccgctgg gagtgtttga ggtgctgggg
    34501 ttgaagtagg ggaataggaa gtagtgaata gtgaatagtg agtagggaat agtgaagagg
    34561 cttccgaggt gcatttgctt cggaagtttt tattgccagg aaccaggaaa agcccctttt
    34621 tcaccacgaa gccacaaagg cacaaagacg caaagaaaaa aacttcgtgc tttcgtgtct
    34681 ccctggcacc gaccgccagg gcaggtgttg agactttgtg tttaagtttt cagacacgct
    34741 tcaagtaaaa aaatccccat tttcaaaaat cgaaaacggg ggaagggacc ataccacttg
    34801 atttaccatt taccatttac catctactat tcactattca ccaatcacta ttcacttctc
    34861 tcccccacca actctacgaa ccgcaccaaa gcttccatgc ctttttcaac ggggtccact
    34921 tccatgaact cttgcggggt gtgggcgctg ccgcctgtgg tgattcccat cactacagag
    34981 gggatgccgc tgctgagcgg gatgtttgca tcggtggagc ctgaggtgag cgcggattcg
    35041 agtccctgtt cgcgggtgca atccacggcc agtttcacca gcgggtgatc agcagggatc
    35101 tcacccgcgg ggcgttcgcc gatgatctca gccagcacac gcacgccggg gcggtctgaa
    35161 tctttgagca ggtcttccac ttgatggatc aatttcaaaa gtgtcttcgg gtcttcggag
    35221 cgcaggtcca actcacattt ggattcagag gccaacacat tgatccccgt cccaccgtga
    35281 atggtgccga cattgagcgt ggtgcgcggc tcccgcggca agcggattcc cgcaaactgc
    35341 gtcaccaggt tggcgagttc atgcaccgcc gaaggcttgc cataatctga ccatgaatgt
    35401 ccgcctgcgg ttttgaccga gatgcgatat ctccgcacac cgatggcgcg atgatagaca
    35461 tgtcccagtg ccagcccttc caacacgaga tacccgatca cattggcgcc aaaccgctcc
    35521 accacggcgc gcattccgcg cagatcgcca agcccttctt cacctacatt tgccacaaac
    35581 cagagatcgt gctgcaggct gagattccgc tcgcgcaacg accagaggat tccaaacaag
    35641 gccgccacac ccagagaatt atccccgatg cccggtgcga cgatgcggcc cgcttctttt
    35701 ttaacctgca agctgatact ggtcggaaaa acagtatcca aatgggcaga gacgatcaac
    35761 ggcttggcct tcttctgttt cccgggcaaa cgcgcataca catttccaag tgaatccatg
    35821 gaaacgtctt tgagattctc ttttagaaaa aggtcacggg tgaattcggc gcgctgtcct
    35881 tcggaaaacg tcggcgcggg gatctgttga atctggatgg caaggtcaat ggtttgggtg
    35941 gcgatgttgt tcatgagggc aagtataaag gcaggcagtg aagtggacaa gtgagcatga
    36001 ataaagagag tggctaaaag ggtgggtttt gattgccaca aagtcacaaa gcctcgaaga
    36061 cacaaaggat tcttagtggc ttagtggctt ggcgtctttg tgtttacccc acgggtttag
    36121 ccactccctg aata
//
LOCUS       DAOQJC010000028        36043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_516_length_36043_cov_10.633044, whole genome shotgun sequence.
ACCESSION   DAOQJC010000028 DAOQJC010000000
VERSION     DAOQJC010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 36043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36043
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_516_length_36043_cov_10.633044"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..2125
                     /locus_tag="PLE39_05960"
     CDS             32..2125
                     /locus_tag="PLE39_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67905.1"
                     /translation="MKKFQNLILLLAFIMTACMPGKPPKQAPQPAPETDSVSTGIPAP
                     QIVPTVQADCAEPICLTASISLLDLQNRSVDENVLKLFSAAVDDENNRVYVAGIMTLD
                     VAVLDGATETWIGTVETGLTERGIKYIYFDSKAQYLYIYDTVRKELRRVDVESGEIVG
                     PVPLAEGGFGQLATVDETHTRFYLTTSKGLVAFDGNTLEQLYTVSGLGESAGPMVYDP
                     AKDVFYLLDTTSKEADRNIFILAAASGEVTGSIHYTAMQGIRSRWLHFDPADQHFIIG
                     MDRSIAIFDMNGTEISAFPFSGEHIVEDQVYDPATKQVFVSSINSPQNGQVAGIGGHM
                     DVYDLSGKRTSSFDFGRKPHHMNLNPANGHIYIPNGDASVVWSVDTHAPTQATSLRLG
                     DSVEQIVPSGDGSTLYFASRLGGSYIGSYNQDSGAFEAFTAGTWPIPIRRDASGEHLF
                     TLNAWDGTLSVYSITPNQTLLGTIPLGTPAGSTDRLPDLAIDSTHKLAYAAYPEFGTI
                     AVVNWETMQPVATIPVEGAETGDTGGGPGQLQVTVNETENRVFVYDSKSRRLWIYDAA
                     NQFSPLGSVDLREASRSIGQSGADLFFFDAQTNRIYLGALELDAVTGQPSGRTLSTGD
                     KIFGLDSASNTYWVFDSASNAILAVDRETLSVRFSQPLPESASFLPTLAYNPARNQIH
                     VGYLATAHVYTFTVR"
     gene            2183..4255
                     /locus_tag="PLE39_05965"
     CDS             2183..4255
                     /locus_tag="PLE39_05965"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNE67906.1"
                     /translation="MRRFIQITLTLLFALALTLGFFSAAPVSPVRAAGVTYYVAVNGS
                     DANPGTLDQPWQTIQHAADSVSAGDTVQVRGGTYNETVSINVSGSDAGGYITFQNYSG
                     ETAILDGSGFSSAAGDIAFTIENQSHISVNGFEIRNYKTNQAGATPMGIFVTGASHHI
                     QILNNYVHHIETNAGANGNAHGIAVYGTAAPASIHDLVIQGNQIRDLKLGSSEALVLN
                     GNVENFIVTQNTVTFCDNIGIDLIGFEGVSPNPSYDQARDGIVSDNTVTAIDTITNPA
                     YGGSQSAAGIYVDGGTRILIERNKIAASNIGIELASEHQGHATSYITVQNNLISYNHI
                     AGLAMGGYDTQRGSTENSTIINNTFFENDQNQDGNGEIWLQYNVNNNTIQNNIVFANS
                     QSWLITNPYTQNQNNVVDYNLYFAPAGGDGSEWQWKNVFYQGFAAYQAGTGNDAHSLF
                     TDPQLTGTTVFDYHLLSTSPAIDIANCANAPTDDFAGDLRPQGAGCDIGADEYTSQLF
                     ADVPADYWARTWIETLYASGITGGCGTNPLTFCPEAPVTRAQMSIFLERGMNGAAYNP
                     PTASGTVFSDVPADYWAAAWIEQLASDGITAGCGNGNYCPEDYVTRDQMAVFLLRAKY
                     GSAYTPPAASGVFSDVPADYWAAAWIEQLANEGITAGCGNGNYCPTQLVSRAEMAVFL
                     VRTFNLTP"
     gene            4343..5443
                     /locus_tag="PLE39_05970"
     CDS             4343..5443
                     /locus_tag="PLE39_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67907.1"
                     /translation="MRLIRTLILITLFAVSCTPTSPPPPPTTATVTESSPTFNIQSPI
                     TSPASISFIINVHDWNHPEESAELLLKLVDLFEQYNIRGDFYFTAEITRQLAEKRPDV
                     IERFKNSNMTISYHVRPPHPLYNGFDAGLQSMSDAELYQTLLDYETYALNLETGELDR
                     SRPGGYTYVAQVFGRNPVVAPVPNSGRIKDAGQKVYASLGAQMTLLYHESGTDLEQPF
                     EYVNGLLVRPSDFSITRTTVIDGADNFWWNFMSKPNADLYDPLKMLQTQLSEWDAHGY
                     NREPFITSLIHENNFYRSGAEAWTSIYFSMQNNKKVQPLSPPYDLNAPDPSMLRTEED
                     QQAIFAAYEKMVAYAAAHLHVVTSEDILEIAE"
     gene            5551..6504
                     /locus_tag="PLE39_05975"
     CDS             5551..6504
                     /locus_tag="PLE39_05975"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE67908.1"
                     /translation="MQRVSFFITLLVISALACGASQSQPSAAVGPVETAPSPQVTEEA
                     GVSSSAFDSQKLGVVERDVTYCIMNGIELKMDVYYPSSNDGPWAAAMYIHGGGWSNGD
                     KADGAGAKEIGPLRAAGFLVVAVNYRLAPQNLFPAMIEDVKCAVRSLRAHADEYNLDP
                     DRIGVWGGSAGGHLAAMLGTTDKNAGFDVGEYLDQSSRVQAVVDMFGPADLTVPFEGG
                     YESARRVFDGFDAARASPVTYVSADDPPFLILHGTEDELVPLEQSQILLARLQAAGVP
                     AELVIVANAAHGFTPAPGGISPSRREISQRVVDFFVKYLGG"
     gene            complement(6589..7017)
                     /locus_tag="PLE39_05980"
     CDS             complement(6589..7017)
                     /locus_tag="PLE39_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67909.1"
                     /translation="MKSDVIKPMLIIMAAPIFSALLSVFKIVPAPPYQGFASLLYLPI
                     CALSLPVAILLGLQKKLLGVNLLVGLIVSFIFPFALFILINPLWMPSGMSNCKEGTPP
                     SATVAHYECEDSSSDDGSYHRDFIIEGYKGWPLMRVIEDK"
     gene            complement(7030..8478)
                     /locus_tag="PLE39_05985"
     CDS             complement(7030..8478)
                     /locus_tag="PLE39_05985"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNE67910.1"
                     /translation="MRHIILLLVLLLTACSSSTAQPIMATLPVETSTPIPPATESPTA
                     TNTPVPIPEREKYIINTFLDYDQHFIDVDQDIIYPNHTGQQLTTLTLAVPPNFWPNCF
                     YLMELQVDGNVVTNYSFNLHRLDIPLPTPLEPDSVSTISLRYSIFLPFMDQVNSQRAR
                     IFGYSDLQMNLVNWYPFVVPFKDGEWIIREPWSHGEYLVYPMADFEVNLKFANPDTAP
                     IIAASGPAEPNGEFTRYTLTGGRAFAISASRDFLVSSTQVGETVVYSYHLPIYQVAGE
                     AAMLTSAQAIQVYSQMFGPYPHKTLSVVVADFQDSMEFSALYFHSRSFYNLYDGTPQS
                     YLVSVAAHETGHQWWFDQVANDQAREPWLDESLTTYSEAIYYEAAHPESLAWWWTNRI
                     DFFQPFGDIDIPVYEGQDADTYKYIVYFNGAHFMRDLRGRIGDAAFFAFLQDYYNQGR
                     GKIVTGDDFFRILDQHTDMDYSDLVSTYFKNR"
     gene            8516..8587
                     /locus_tag="PLE39_05990"
     tRNA            8516..8587
                     /locus_tag="PLE39_05990"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8549..8551,aa:Arg,seq:cct)
     gene            complement(8785..10521)
                     /gene="recN"
                     /locus_tag="PLE39_05995"
     CDS             complement(8785..10521)
                     /gene="recN"
                     /locus_tag="PLE39_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNE67911.1"
                     /translation="MLTELHIQNFAIIDKLDLRFDKGLIILTGETGAGKSIILDAVVM
                     LIGGKADATFVRSDSDAAFVEAVFQLSGPEKEAVTEVLTREELLDDPNYVVLMREVRK
                     EGRSVARVNGRTVNVGLLKELGALLIDIHGQAEHLSLLDPRAHLGLLDRYAEVVKPLG
                     DYRQSYQALLGLRRELVELRKAQSDSERRTEMLTYQAEEIEDARLKAGEDEELRKERD
                     RLANAESLAQNAQEALAILDEGSPDMPAATDLIGQAAQALAALAKIDPEQVELSNQAE
                     LMLDTISDVVHGLQNYLEEIEFNPKRLDEVEERLNLIHSLTRKYGGSISSVLAYGADA
                     RKQLETLTNAADRINELEMQEAKLLDKLAKLGGVLSEKRKSAALTMSKGIEAELGDLR
                     MEAARFSVDFQTKPDVNGIPLADGTRIAFDQNGFDRVEFLIAPNPGEGLKPLAKIASG
                     GETSRLMLGLKNVLARADQVPSLIFDEIDQGIGGRVGMVVGQKLWNLSRSHQVFCVTH
                     LPQLAVFGDQHYQVQKLVDQGRTLTRVERLEGEPRLLELSQMLGEVGEGTLRSAHELL
                     QTARQMVKTPKK"
     gene            complement(10525..11100)
                     /locus_tag="PLE39_06000"
     CDS             complement(10525..11100)
                     /locus_tag="PLE39_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67912.1"
                     /translation="MTDTEITPTATYCYAHPGRETSLRCKRCEKPICASCAQRTPTGY
                     MCKDCVNQHQKIFDTAVWYDYLTVFFTSAILSGLAAVATIIITSIIWGFFIIGLAPLA
                     GTMIANVTRRFVKNHRSPMLNTTLVVGMILGALPVLLLSGLPGLLTLLGGGGNVMGSV
                     VSFSPVLWQIVYLVIAAPVAYSQFSGLVFRR"
     gene            complement(11097..11960)
                     /locus_tag="PLE39_06005"
     CDS             complement(11097..11960)
                     /locus_tag="PLE39_06005"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNE67913.1"
                     /translation="MPDPFLPKNPVVTAYPKMPEAFTEAEAMVSFLKERGIQAPFGSL
                     YDEELRRRVKNGEFDMLIAVGGDGSVLRAGHLCAPCGVPILGVNLGRLGFLIQIDRKE
                     WREYFEKLFEGEAWIENRMMLRAEHIRAGESINRSNALNEVVVARGQNLRPVRLTASV
                     DNRRLTSYVADGLIASTATGSTAYALAAGGPILPPELRNILLVPIAPHLSVDRAVVLS
                     EGSSVSIVVKSENSVLSVDGQPPTPLMEDDQVKVTAADVTAQFVRFGDPGYFYRNLTA
                     HMNENSLGFPR"
     gene            complement(12003..12515)
                     /gene="cas4"
                     /locus_tag="PLE39_06010"
     CDS             complement(12003..12515)
                     /gene="cas4"
                     /locus_tag="PLE39_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HNE67914.1"
                     /translation="MLYLALALFLFALFFFRKSSVQRKEAGLPGGRIIYTDTRGWGKV
                     EKPLFYPALELTGKPDYLVEKNGQIIPVEVKSGRAPQAPYDSHIYQLAAYCLLVEKTY
                     GKRPPYGIIHYENRDFAVDYTHELETALIDLLADMKLDEHKNDVPRSHEQAARCAKCG
                     FRNMCDQSLA"
     gene            complement(12534..13166)
                     /locus_tag="PLE39_06015"
     CDS             complement(12534..13166)
                     /locus_tag="PLE39_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67915.1"
                     /translation="MSAVLSRLAHLQTRRDRTPNLDLARDLAARNDKAGIREIAQNMW
                     SANKNVHWDCIHVMYEIGAIDPALIAPYAEDFVKLLKSKHNNMVWGAMTALAEVAKVK
                     PEVIFKHLDEIKKAKENGSVITVDNSISVLAQTAAANEEYNKAIFPYLLKHLSACRPG
                     EVPQHAERTLPAVNESNRDDFIKTLNRRNDDLSGAAFTRLGRIIRIAENI"
     gene            complement(13188..13454)
                     /locus_tag="PLE39_06020"
     CDS             complement(13188..13454)
                     /locus_tag="PLE39_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67916.1"
                     /translation="MAIENLCYTLAMPIIRASDIGNYLYCRRSWWYRKQGFESANKAE
                     LAAGTELHSRHGRQALASSLSRTVGLILLLGALILLVAYCTAQL"
     gene            13526..14077
                     /locus_tag="PLE39_06025"
     CDS             13526..14077
                     /locus_tag="PLE39_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNE67917.1"
                     /translation="MTKHQVHPIEIAGIKRELPLFEVKPGLRIAILNILGDTELVQAC
                     ARELGKKLQAVDYDILVTAEAKSIPLAYALSVETKKPYVILRKTYKPYMGAALEAETL
                     SITTGQPQVLILDEKDRSIIAGKKVVILDDVISTGSTLQGMRMILDKTDAKVVAEAAI
                     LTEGDRAQWMHIISLGHLPLFTG"
     gene            complement(14140..14397)
                     /locus_tag="PLE39_06030"
     CDS             complement(14140..14397)
                     /locus_tag="PLE39_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNE67918.1"
                     /translation="MTCYCYIVECSDGTYYTGWTTDPERRVSQHNKGIGAKYTSTRRP
                     VKLVYLEIQPDRTAAMKRELAIKRLKREQKARLIAGDLKPK"
     gene            complement(14394..15380)
                     /locus_tag="PLE39_06035"
     CDS             complement(14394..15380)
                     /locus_tag="PLE39_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNE67919.1"
                     /translation="MPTQITIIGLGQIGTSMGMALAAHKASIVRVGHDKKAELERDAQ
                     QKDAIDKAEHNLPTAVRDARIVVLTVPVSQVRDTLEFIAPDLKEGTVVLDTSPIKANV
                     AKWAKEILPADRHYVGLVPAINPVYIHDFQFGQAAAKPDLFANGLFLIDAPYGTPESA
                     VTTAVDFVRLLEALPMLTDPAESDGLMSTTHLLPQIVAASLLNATVDQPGWQEARKVA
                     GRPFATVTAGLAYQDEIDSLRLSSLQNRAGMIHALDVMIAALRGFREDIENEDDEGVA
                     ARLEAALEGRQRWLSERVAADWSGATRSEAADMPTFSERFFGGMFVKKHNRK"
     gene            15621..16145
                     /locus_tag="PLE39_06040"
     CDS             15621..16145
                     /locus_tag="PLE39_06040"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNE67920.1"
                     /translation="MTNLPLKIYLGELNHTPLGDLRIAASDLGLVAVEWAESQPRLFE
                     YLFRLKGTVEHSQKATHRYTKELGEYLKGKRHDFTFEINWGTLKPFQRKALKAVYQIP
                     YGETLTYADIAMQIGHPKASRAVGRANATNPMPLVIPCHRVIGKDGKLHGYGGGDGIP
                     TKEWLLKLEGAVIA"
     gene            16202..16720
                     /locus_tag="PLE39_06045"
     CDS             16202..16720
                     /locus_tag="PLE39_06045"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNE67921.1"
                     /translation="MNILTELFWTFIKVNLLSTSGPASVGLLYHEAVGRFVTESQFVQ
                     AVGFSSVLPGSDALQLAMYIGYAAGGIPGGLVALLASILPPTLIILGVTMILHRLRRE
                     AWVGHFVEGLAPAISVLMVFTAWKIFEKGGETGPLGWAIGILSLIAMWLKAPERIVVI
                     LAGIAGIILLSP"
     gene            16717..17253
                     /locus_tag="PLE39_06050"
     CDS             16717..17253
                     /locus_tag="PLE39_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNE67922.1"
                     /translation="MKQSKITVWSSLLWMFLKVNLLSPSGPASVGLLYKEAVGKIMTE
                     EQFVEAVGFSNVLPGSEALKLAMFVGFAAGGVPGVLTALLGAVLPPTVMMLAVAFVLQ
                     KFQQEDWLQGFVKGMSPAIAALIIVVAWELFHVGQTKKIDRRTLFIAAVSAVALWFDL
                     PSPLVLLGAGVLGVFLFR"
     gene            17265..18110
                     /locus_tag="PLE39_06055"
     CDS             17265..18110
                     /locus_tag="PLE39_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNE67923.1"
                     /translation="MTQNVLIATNGFKGTWPSIEYGTWLASTLNTNVTLLGVNEKPNP
                     AAIDDHNPLEDVFEAAVELFQKNGVKYELLVENGNAEDVIPQWAKQRDSIVVLGPLGR
                     PQIRRLLTGRSIRHLMEEIEQPILYVPEATLPLKKILICIGGLGYEVTAEHIAMQMAM
                     KSRAEVTLLHIVPPMDLDYPTARIVRENWQNLTETDTPVGRSLREALQVAKDEGLTAS
                     VKARQGNVVEEILAEVKEGGYDLLCMGSPYSTNALRQLYAPNVTAEIAEAVTCPVLSA
                     RYKRE"
     gene            complement(18118..18855)
                     /locus_tag="PLE39_06060"
     CDS             complement(18118..18855)
                     /locus_tag="PLE39_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67924.1"
                     /translation="MELQFATVTWPVPAVWGVGLVVLLIGFFIGFLDSNIRTNKKITA
                     AEVQAEAKIREAENKMILASQEESVSQKKDDPGLLRLKKENAHYTLEMDGRVVNKPPS
                     SEEKKRLIELITIFRPWLDNSPAPASERPAAPVQPAPTPAPVQATSPRPMPPIVTVAA
                     QAELDKSIRAMSIVNQIDTVLQARLVGTPLEKAGIRLQESPEGGVEVIVGIETFPTVD
                     DVRDAVIKREIRAAIAEWEDKYVPGAR"
     gene            complement(18859..19668)
                     /gene="mutM"
                     /locus_tag="PLE39_06065"
     CDS             complement(18859..19668)
                     /gene="mutM"
                     /locus_tag="PLE39_06065"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNE67925.1"
                     /translation="MPELPEVETFARSLKPELVGNTILSADLRWARTLATPSPSQFKQ
                     RIIGQTIVDVSRRAKYLIVHLKDYNLLIHLRMSGDLIVRTGKIKPEKHDRLLLHLSNH
                     TALAFNDTRKFGRVWLTDQPEKILGKLGPEPLGNDFTAQWLFENLHTRKRQLKPLLLD
                     QSFIAGLGNIYTDECLHIAKLHPQALSNTVSQEKAQALHKAIRAVLKEGIRRNGASID
                     WVYRGGDFQNHFRVYDQDGKPCPVCGTTIQKLTVGQRGTHVCPKCQAIERK"
     gene            complement(19705..20694)
                     /locus_tag="PLE39_06070"
     CDS             complement(19705..20694)
                     /locus_tag="PLE39_06070"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNE67926.1"
                     /translation="MHHVHRDLKVYGGIKLFAGTGSPDLAQKIADYLDQPVSSREVIE
                     FPNENLFVKLNGSVRGQDVYVIQTTSSPVHRNLMELLIMIQTLRLDSAARITAVIPYL
                     CYGRSDKKDQPRVPITARLVADMLEVAGADRYMTLDPHAGQVQGFFSIPGDVLTASHL
                     LVDHINNNMRSQMKDPVVVSVDLGFAKKGRNYAADMDMPIAFIEKRRHGNDAKAEALT
                     LIGDVKDRDVIIVDDEVDTGGSISQAVNVVKQNGARDVYLAFVHAILSRNGAERLASL
                     PIKHIITTDSAPLTPEKWKFLESRTTVLSVAQLLGEVIRRAHEGRSVGEMFNE"
     gene            complement(20695..21249)
                     /locus_tag="PLE39_06075"
     CDS             complement(20695..21249)
                     /locus_tag="PLE39_06075"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNE67927.1"
                     /translation="MSIIYGKRIRLRAAEREDVATFHKWVNDPEVTRHLVLSLPMSMV
                     DEENWFNSMNQRPQNEKVLVIEIREGEGWRMIGNCGVFAVDSVSRCGELGIMIGEKSE
                     WNKGYGTETIALLAQHCFETLNFNRVFLQVYAENERGKHAYEKAGFVEEGRLREAVYK
                     HGKYDDVFVMSILRSEWNAKKGKE"
     gene            complement(21249..22277)
                     /gene="meaB"
                     /locus_tag="PLE39_06080"
     CDS             complement(21249..22277)
                     /gene="meaB"
                     /locus_tag="PLE39_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNE67928.1"
                     /translation="MTYSQAVLNGERLALSRLLTQVENDAPESRAALIELFPHTGKAH
                     LIGVTGSPGTGKSSLVNQLALHYRKTAQKRVAVIAVDPSSPFTGGAVLGDRVRMRDLS
                     GDPGVFIRSMATRGSLGGLAQSTANMAQVFDAAGYEVIIIETVGAGQSEVDVARLAHT
                     TLVVEAPGLGDDIQAIKAGILEIADILVVNKADRPGVENTEKALKSMLDLAHPTERIF
                     QHHGTAMRISSGPTSGGPDSKQEASSAPIWIPPIQRTVSTEGKGIVELTDAIARHVAH
                     LTQSGDWAVRERARLEVELDALIQETLVSRFREKVSQGQYENALERIFQRKISPWEAV
                     KSLMDGDA"
     gene            complement(22421..22819)
                     /locus_tag="PLE39_06085"
     CDS             complement(22421..22819)
                     /locus_tag="PLE39_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNE67929.1"
                     /translation="MTEKKIRVLVAKPGLDGHDRGAKVVARALRDAGMEVIYTGLRQT
                     PEMIAEAALQEDVDVVGLSILSGAHMALTPRLLELLKMNGQSHVKIFVGGIIPDEDLH
                     RLKEMGVTGVYGPGASTEDIIRDVKLAVAG"
     gene            complement(23111..25438)
                     /locus_tag="PLE39_06090"
     CDS             complement(23111..25438)
                     /locus_tag="PLE39_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNE67930.1"
                     /translation="MPLLKPSTPPKKTAKPAGKTIPPKKGEAPQNHPAQKGKGQSPAP
                     TPPPPPTPALSAWDRLSPERKLDVVGIILAFFGVIIILGLASANRSAVVGGIIFFIAQ
                     IFGWGVYILPLGLLIFGLWLVFRKIERIPPLSIERVVGSVILFLWLLIVLHTFVATAE
                     TAEAEALKGIGGGYIGGKLQWFLWVGFGGGGAFIALVAWLVIGIAVLLDKPVQDLFFW
                     LGPIFTWIRNLLNKPIASDSPAPASASTEDLTPIDPSAMPDVKPAAGIGQPTRTTSGA
                     TVIHWTLPNVSDILDAGNAPSINEDFIQQRARLIEETLASFGAPAQVVAISRGPSITQ
                     FGVEPLFLESRGGVKTKVRVSKIASLSDDLALALAAPRIRIQAPVPGHSYVGIEVPNE
                     EMAMVALRDILEGEVYKRNTKPLNFALGRDVAGLPVIASIESMPHLLIAGTTGSGKSV
                     CVNSILTCMLLYNTPDDLRLVLVDPKRVELTGYNGIPHLLGPVVVEMDRVIGALQWMT
                     REMDKRYHMFAQVGSRNITDYNAKMKLKGEKKLPFLVVIIDELADLMMIAPDETEKTI
                     TRLAQLARATGIHMILATQRPSVDVVTGLIKANFPARIAFAVASNTDSRVILDQPGAE
                     RLLGRGDMLYQAPDAPSAARLQGVYVSDHEIQSLVEYWRNQAGGASPYAVAGSNPADA
                     VPTNVPLKQAPLWEDMTKVEGDPLFDDAVEIVRKEGRASVSMLQRRLRIGYTRASRIV
                     DTMEDKKIVGPPEGATQMRAVLDYGQTAPPKDDGM"
     gene            25737..26669
                     /locus_tag="PLE39_06095"
     CDS             25737..26669
                     /locus_tag="PLE39_06095"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNE67931.1"
                     /translation="MFEILFLGTSASAPSAKRGLSGQIVSHNEYRFLVDCGEGTQRQI
                     LQSGIGFKRLTRILLTHGHLDHILGLGGLLSTFLRWEAIEELEIYAGHSTLERVRTLL
                     YDVVLRGNQPPMPLILKEIKPGVIFEAEDFTVTAFSVTHRGPDCLGYIFEEKPRRPFL
                     SQKADELGVPFGPERRDLVEGREITLANGTRIKPDEVLGDWEKGSKLVLVGDAGRTDN
                     LLEVCKDADALVIESTYLDEEADMAKQFSHLTARMGAELAQKAGVKKLILTHISRRYR
                     EKDVIAEAQSIFANTVVARDFDNYQIRKETEHGR"
     gene            26659..27633
                     /gene="pfkA"
                     /locus_tag="PLE39_06100"
     CDS             26659..27633
                     /gene="pfkA"
                     /locus_tag="PLE39_06100"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNE67932.1"
                     /translation="MADKFTVGVLTSGGDAPGMNAAIRAVVRTALTNNMKVIGVHNGY
                     EGLINGEYHQLDARAVGGILQRGGTILLTSRSKRFQEPTGQRDAIRKMNEAGMDALIV
                     IGGEGSMNGAYALSQKGVKVIGIPGSIDNDIWGTNIAIGTDTAMNTIMEAVDKLRDTA
                     SSHQRAFVIETMGRNSGYLAVMAGIVCGAEMVLIPEVPVTGEEVAAAVEDAYQRGKTH
                     AIIINAEGSAIRTTDLVEKIDTLDLGFKTRMTILGHIQRGGSPTAYDRLLASRMGVKA
                     VEALVEGQHGVMTGLKGKGVDFIPLLDVISNKRKVNMEYYHMTKVLAR"
     gene            complement(27710..29071)
                     /locus_tag="PLE39_06105"
     CDS             complement(27710..29071)
                     /locus_tag="PLE39_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67933.1"
                     /translation="MNGTKERLMILDMIAEGKITAAEGEELFKVLEEGDEPSAEPDFP
                     MPGPPSRPFPPLRPEPPQSPRGQRASTELVAALKTAGIDHVTLSDIQEMQDHNLTAEY
                     VHEMLALGIEPDGLGEWINLRMHDITPRYVRELRDMGVDDLDMDELIELSLHDVSAKY
                     VSELREVGLKDLDVDELVELSNHEVSAKYIAEMHELGLKDLDVDELIELNDHEVSARY
                     VAELRAMGLKDFDVDDLIELSNHEISPKYVAELKKMGFKNFDVDDLVELGVHEISPEF
                     IAELQTLGIKDLDIDDLVELGTHGVSPEFIAQMRESGLKNLDTDDLVQLSIHNIDPEF
                     MKALRDFGITGFDVDDVVELGIHNVTARYLTEMKEAGLKDVDTEELVEMRIHNVTPKF
                     VRELREMGFSNLPADELVELSIHHVTPRFIREMRQRYGENLTLEQLLDMRLHGVEEAL
                     GSR"
     gene            complement(29068..29454)
                     /locus_tag="PLE39_06110"
     CDS             complement(29068..29454)
                     /locus_tag="PLE39_06110"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HNE67934.1"
                     /translation="MPLIPQTCPSCSSPLVVTQLNCTSCGTGVVGKFELSPFSRLSPE
                     RLHFLEIFVRNRGNVKEMERETGESYWAIRRQLDEVITEMGFEETPKEDDPSTRRQEI
                     LARLSRGEINVQEATKLLAQIKGDPA"
     gene            29758..33513
                     /locus_tag="PLE39_06115"
     CDS             29758..33513
                     /locus_tag="PLE39_06115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE67935.1"
                     /translation="MSSEPNEYNALNEAPAQDTLTGAYTRATFKASLHALIEQAQRDN
                     TTCSLMVIDLDHFKSINDAFGHAKGDEVLSGAAHLILQNTRASDLFFRYGGDEFVLLL
                     PNTTKTEAFDLAQRLLGIIRAQSFSRDTVLTLTLSIGVAAFPEDASDGQSLFEKADAR
                     NYEAKRRGRACVISRDGGTTSDLSFIKPSRLIERDASLELAQNFLSALVSQQHGVFGV
                     IGPNGVGCSRFLVEVEKRARLQGFEVIALRGTPQLMARPYGVLENILQNPAGAAFTQN
                     NFEKTIKAFQRRLTESGRDRLLISVDDLPQLDWGTLDLLRQILSSNEFPVLGLVYATD
                     PDHARVTAPFRAALSEYVTLSPLTPAGLQVWLRTLLQWDPPNDFIHWLEKHTHGLPAQ
                     AEQVLKHMLKEGALTRQDSGWHLSENYATIGAAGMRQWQKRVSPNNLPAALTSFVGRE
                     TELKDVRQLLATTRLLTVVGPGGMGKTRLTLQVAKEAINGFRNGVWLVELAPVTDPAL
                     ALYTIATVLGVEQVQSHSLMETVCTWLSDKEILLILDNCEHLIDMCAQFSHEVLSTSP
                     SVRILASSREALDIAGELVYRMPALETPNIQAEVPAGQLMQYAAARLFAERAAFALST
                     FRVNAEEAPLIAQICRQLDGIPLAIELAAARLRTLTLEEIATGLNDRFNLLTRGNRAA
                     APRQQTLRALIDWSYDSLSENERRLFCRLSIFQGSWTQDAIGVICSGDGIESHDISNL
                     FTQLINKSLIEAIERSTDKEPRFRMLETIRQYAYEKLLETEGSETIRQKHLAYFMGLV
                     ERAAPNLRTFDMLIWLDRLEDELDNIRAALEWGLKSDIETVLRLASALLWFWHIHSHQ
                     NEATDWLKQGLAREANERGGNPIGPQRALLRGNALNTISFLMRNSEQRITLLEESLSL
                     FRELGVQGRQGLATALRILGNSELEKGHNKQARAFVEEGLSIFMEIREPFDMAECLDL
                     LVSLAMDEGDFERARGLQEEALSLRKEIGDKDGTAYALYILGQIAHRQNKAALAQALY
                     EESLKLFREIDNRTWVADITGTFAVLASAQGNRKKTNLLIEEGLALARDLDDKYGLAH
                     LMLNLGEAMRFQGDLDRASQLYEEALVLSQRMDANNFISSVLYGKAEIARSQNDLEQA
                     YALHCEALNIRQKISHTYGIVRSLNAIAELTILQGNSPQAVRLFGAIESMYKTSRPPT
                     EQAGHDRAVSAARSQMGETMFNSIWSEGQKLPLDQAISLVLTKRSAT"
     gene            complement(33506..>36043)
                     /locus_tag="PLE39_06120"
     CDS             complement(33506..>36043)
                     /locus_tag="PLE39_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNE67936.1"
                     /translation="NRGMQKYLPVLRARRERRLQKQNKQANNARGAILSGGIVLSLLL
                     GVFIILGAFAYADVTRELPSVEILPRLLNPPDGLLLEPTHIYDRTGTHILFTFAPVDE
                     QRRYIPANPQNPQHIPANLIDAIIAVQDPQFRKHSGYSLQGLDDANQHPTIAQKLIGG
                     LVIYNEPPSLRRAIRERILAAQITAQFGRTQVMEWYLNSAHFGNYAYGVEAASQLYFG
                     KAVEQLTLAESAILAAASESPALNPLDAPQVALQRGREVLYVLNGLGIVSDEATEIAL
                     AEEPEIRASTEDDANQPDSEPAPAFVNMVLAQLDTQLPRDRVERGGINIITTLDYDLQ
                     QQAACVTEVYAVRMAGLEEPKEACDAARFLPSLPPAVTLTDSSASALILDPNHGQVLA
                     VVGETVQGQETPLVTAHSPGSSLDAFVYLTGFTRGLSPATLAWDIPGEGIIQNFDGEY
                     HGPVRLRLALANDYQVPMEALKVQMGVENVSGIASSFGVALDKQVTMLQLAGAYGVFG
                     KQGVYFGQNMNDEFLPSAVLRVEGVDRSLLLDWSLPQAKPVLTPALAYLMTHVLSDEA
                     SRWPSLGRPNVLEIGRPAGVKVGQSANGLDAWVVGYTPAHVVVTWTGVHGEAESVTPR
                     FPAALWSALMKVASGDAPADGWTAPQGVSTITVCDPSGMLPTKECPRLVSEVFLNGSE
                     PVQADTLYREFGINRETGLLATVFTPPELIETRIYMLVPESARDWALSAGLEIPPASY
                     DAIQAPPVNPFANIVEPKLFAEVGGVVKIMGTAAGENFQYYRVQVGKGLNPQEWVQLG
                     GDVISPVEDGLLAEWDTSGLSGLYAVQLLVVRADQIVDTAVIQVTIK"
BASE COUNT         8722 a         9662 c         9369 g         8290 t
ORIGIN      
        1 gcacgaaaaa caataaaaca cggagaccac catgaaaaaa tttcaaaacc tgatcctgct
       61 cctggccttc atcatgacgg cctgcatgcc ggggaagcct cccaaacagg cgcctcaacc
      121 cgcgcccgaa actgattctg tttcaacagg cattcccgcc ccgcaaatcg tccccaccgt
      181 tcaagccgat tgtgccgaac cgatctgcct caccgcttcc atttccctgc ttgatctgca
      241 aaaccgcagc gtggacgaaa acgtgctcaa gctattctcc gccgcagtgg atgatgaaaa
      301 caaccgcgta tatgtggcgg gcatcatgac cctcgatgtg gccgtactcg acggcgcaac
      361 cgaaacatgg atcggcaccg tggaaacggg tctcactgaa aggggaatca aatacatcta
      421 ctttgattca aaagcgcaat acctttacat ctacgatacg gtccgcaagg aattgcgccg
      481 tgtagatgtg gaaagcggcg aaatcgtcgg ccctgttcca ttggcggagg gcggcttcgg
      541 ccagcttgcc acagtggatg aaactcatac ccgcttctat ctcaccacct ccaagggcct
      601 cgttgctttc gatggcaaca ccctcgaaca actctacact gtaagcggac tcggcgagag
      661 tgccggtccc atggtgtatg acccagccaa agatgtcttc tacctgctcg ataccaccag
      721 caaggaagcc gaccgcaaca tcttcatcct tgccgccgca agcggagaag tgaccgggag
      781 catccactac accgcgatgc agggcatccg ctcgcgctgg ctgcacttcg acccagccga
      841 ccaacacttc atcattggca tggatcgcag catcgccatc ttcgatatga acgggactga
      901 gatcagcgcc ttcccgtttt ccggtgaaca catcgttgaa gatcaggttt atgaccccgc
      961 cacaaaacag gtcttcgtct cctccatcaa ctccccgcaa aacggacagg tggcaggcat
     1021 cggcggccac atggatgtgt acgacctttc aggaaaacgg acctccagct tcgattttgg
     1081 acgaaaaccg caccacatga acctcaaccc agccaacggg catatctaca tcccgaacgg
     1141 cgatgcctca gtggtctgga gcgtggatac acatgcgccc acacaagcca cctccctgcg
     1201 cctcggcgac agcgtggaac agatcgtccc atccggcgat ggcagcaccc tgtattttgc
     1261 cagccgcttg ggcggcagtt acatcggcag ctacaatcaa gatagcggtg catttgaagc
     1321 cttcactgcg ggcacatggc cgatccccat ccgacgcgat gccagcggcg aacacctctt
     1381 cacactgaat gcctgggatg gaaccctctc tgtttactcg atcaccccaa atcaaaccct
     1441 gcttggcaca attccacttg gaaccccagc aggttccacc gaccgcctgc ccgatcttgc
     1501 catcgactcg acccacaagc tcgcctatgc cgcctacccg gaattcggca ccattgccgt
     1561 ggtcaactgg gaaacgatgc agcctgtcgc cacgatccct gttgaagggg cagaaacagg
     1621 cgacaccggc ggaggacccg gtcaactgca ggtgactgtc aacgaaacgg agaatcgtgt
     1681 cttcgtctac gattcaaaat cccgccgact gtggatctac gatgccgcaa atcaattctc
     1741 tccccttggc agtgtcgact tgcgcgaagc gtcccgctcc atcggccaat caggtgccga
     1801 cctgtttttc ttcgatgccc aaaccaatcg catctatctc ggcgcacttg aattggatgc
     1861 ggtcacaggc cagcccagcg gacgcacact gagcacaggc gacaagatct tcggcttgga
     1921 ttccgcttcg aacacatact gggtgtttga ttctgccagc aatgccatcc ttgcagtaga
     1981 ccgtgaaact ctgtcggtgc gattcagcca gccgctgccc gaatcagcat cctttttgcc
     2041 caccctcgcc tacaaccccg cacggaatca gatccatgtc ggctacctgg ccacggcaca
     2101 tgtgtacaca ttcacggtcc gctaacgcaa cccaagcctg ttagaatcca tgcacaacaa
     2161 ttgcacgcga aaggaatctc acatgcgccg tttcattcaa atcaccctta ccctgctctt
     2221 tgctctggca ttgacccttg gctttttcag cgccgccccc gtcagcccgg tccgcgccgc
     2281 aggggtgacc tattacgttg ccgtcaatgg cagcgacgcc aaccccggca cactcgacca
     2341 gccatggcaa accatccaac acgcagcgga ttccgtcagc gcgggtgata cagtgcaggt
     2401 acgcggtgga acttacaatg aaacggtctc cattaacgtc tctggttcgg atgcaggcgg
     2461 ctacatcacc tttcaaaact attccggcga aaccgccatt ctggatggca gcggattttc
     2521 ctccgctgcc ggggatatcg ccttcaccat tgagaatcaa agccatatca gcgtgaacgg
     2581 gttcgagatc cgcaactaca aaaccaatca ggcaggcgcc acaccgatgg gcatttttgt
     2641 caccggcgca tcgcatcata ttcagatcct caacaattac gtccaccaca tcgaaaccaa
     2701 tgcaggcgcc aatggcaacg cacacggaat cgccgtctat ggcaccgccg ctccagcctc
     2761 gatccacgac ctggtaattc aaggcaacca gatccgtgac ctcaagctcg gcagcagtga
     2821 agcgctggtc ctcaacggca acgtggaaaa tttcatcgtc acccaaaaca cagtgacctt
     2881 ctgcgacaac atcggcattg acctcatcgg ctttgaaggc gtttcaccca acccatccta
     2941 cgatcaagca cgggatggca tcgtcagcga taacaccgtc accgccattg acacgatcac
     3001 caacccggcc tacggcggct cacaaagcgc agcaggcatc tatgtggatg gcggcacacg
     3061 catcctcatt gaacgcaaca agatcgccgc ctcgaacatc ggcatcgaac ttgccagcga
     3121 acatcagggc catgccacca gctacatcac cgtgcaaaac aacctgatat cctacaacca
     3181 cattgccggg cttgccatgg gcggttacga cacccagcgc ggcagcactg agaattccac
     3241 catcatcaac aacaccttct tcgaaaacga ccagaatcaa gatgggaacg gcgagatctg
     3301 gctgcaatac aacgtcaaca acaataccat tcagaacaac atcgttttcg ccaattcgca
     3361 aagctggctt atcaccaacc cctacaccca aaatcaaaac aacgtggtgg attacaacct
     3421 gtactttgcc cccgcaggcg gcgatggcag tgaatggcaa tggaagaatg ttttttatca
     3481 gggcttcgcc gcttatcagg caggtaccgg caatgacgcc cattccctgt tcaccgaccc
     3541 gcaattgacc ggcaccacgg ttttcgatta ccacctgctt tccacctcac ccgccataga
     3601 cattgccaac tgcgccaacg ctcccaccga tgacttcgcc ggtgatcttc gtccgcaggg
     3661 tgcgggctgc gatatcggcg ccgatgaata caccagccaa ctcttcgccg atgtccccgc
     3721 cgattattgg gccagaacct ggatcgaaac cctctacgcc tcgggcatca ccggcggctg
     3781 cggaacgaac ccgcttacct tctgccccga agccccggtc acacgcgcgc aaatgtccat
     3841 cttcctcgaa cgcggcatga acggcgccgc ctacaacccg cccaccgcca gcggcacggt
     3901 cttcagcgat gtccctgccg actactgggc cgccgcatgg atcgaacaac tcgcctccga
     3961 cggcatcacc gccgggtgcg gcaatggcaa ctactgcccc gaagactacg tcacccgcga
     4021 ccaaatggcc gtcttcctcc tgcgcgcaaa atatggctca gcctacaccc cgcccgccgc
     4081 cagcggagtc ttcagcgatg tccctgccga ttactgggcc gccgcatgga tcgaacaact
     4141 tgccaacgaa ggcatcaccg ccgggtgcgg caacggcaat tactgtccga ctcaacttgt
     4201 gagccgcgcc gaaatggccg tcttcctcgt caggaccttc aacctcacac cataaaatcg
     4261 tacggcaaaa ttcattttgc accagcgtgg caaattccgc cccgctctct tcaacgcacc
     4321 ttttttacga aaggactccg aaatgcgact catccgcact ttgattttga tcaccctgtt
     4381 cgcagtttcc tgcaccccca catcgcctcc gcccccgccg acaactgcca cagttaccga
     4441 atcatctccc accttcaaca ttcaatcgcc aataacatcg cccgcctcca tctccttcat
     4501 catcaacgtc cacgattgga atcacccgga agaaagtgcg gagcttctgc tcaaactcgt
     4561 tgacctcttt gagcaataca acatccgcgg cgatttctat ttcactgccg agatcacccg
     4621 tcaacttgcc gaaaaacgcc ccgatgtcat cgagcgattc aaaaattcga acatgaccat
     4681 cagctaccat gtccgtccgc cacacccgct ttacaacggc tttgatgcag gcctgcaatc
     4741 catgagcgac gccgagttgt atcaaaccct gcttgattat gaaacctacg ccttaaacct
     4801 cgaaaccggt gaattggaca ggtcccgccc cggcggttac acctacgtgg cacaggtctt
     4861 cgggcggaat cctgtcgttg cccctgtgcc caacagcgga aggatcaagg atgcggggca
     4921 gaaagtctac gccagcctcg gcgcacaaat gactttgctt tatcacgaat cagggaccga
     4981 tcttgaacaa ccgtttgaat acgtcaacgg gttgttggtg cgccccagcg atttcagcat
     5041 cacccgcacc acggtcattg atggcgccga taatttctgg tggaatttca tgtccaaacc
     5101 caatgccgat ctgtacgacc ccttgaaaat gctgcaaacc caactgtcag aatgggatgc
     5161 gcacgggtac aaccgtgagc ctttcataac gtcgctgatt catgaaaata atttctaccg
     5221 ctcgggagca gaagcctgga catccattta cttttcgatg caaaacaaca aaaaggtcca
     5281 accgctttcg ccgccctacg atttaaacgc cccagatcca tccatgctcc gcacagaaga
     5341 ggatcagcag gccattttcg cggcgtatga gaaaatggtc gcatatgccg ccgcacatct
     5401 gcatgtggtc acatcagaag atattctgga aatagcggaa taagtcattt gcagggatcg
     5461 aatttcaaac cgccgcagca caagaacaca taaaaaaatc ggtcataagc gatgactgtc
     5521 actttatttt caagcacaca aaggatgatc atgcaacgag tttctttctt tatcacatta
     5581 ctggtaattt ctgcactggc ctgtggcgcc tcgcagagtc aaccgtcagc ggcggttggg
     5641 cctgttgaaa cggcacccag tccacaggtc acggaggaag cgggggtttc atccagcgca
     5701 tttgactcgc agaagcttgg cgtggtcgaa agggatgtaa cctattgcat aatgaatggg
     5761 atcgagctga aaatggatgt gtactatcca tcttcgaatg atgggccgtg ggcggcggcg
     5821 atgtacattc atggcggggg ttggtcaaat ggcgataagg ccgatggtgc aggcgcgaag
     5881 gagatcggac ccttgcgggc ggcggggttt ttggtggtgg cagtgaacta tcgtctcgcg
     5941 cctcagaatc tcttccctgc aatgatcgaa gacgtgaaat gcgcggtgcg ttcattacgc
     6001 gcccatgccg atgaatacaa ccttgaccca gaccgcatcg gtgtgtgggg cgggagtgca
     6061 ggcggacatt tggcggccat gctcggtacg acagataaaa atgcagggtt cgatgtgggc
     6121 gagtatctgg atcaatccag ccgtgtgcag gctgtggtgg atatgtttgg ccccgctgac
     6181 ctgactgtgc ctttcgaggg tggatacgaa tcggcgcgcc gtgtgttcga tgggttcgat
     6241 gcagcacggg ccagcccggt tacttatgtt tcagcagatg atcctccatt tttgattctg
     6301 catggcacgg aggatgaact ggtaccgctt gaacaatccc agatcctgct tgcgcgtctt
     6361 caagcggcgg gtgtcccagc agaattggtc attgttgcaa acgcagccca cggatttacc
     6421 cccgccccag gcggcatcag cccatcgcga cgagagatca gtcaacgggt ggtggatttt
     6481 tttgtgaagt atttgggagg atagttttca ggcaggaaaa attcccgacc ctgctgctcc
     6541 tgccgtagaa cgtggcaaaa gcagcagggt cgtttgcttg catacaggtt atttatcctc
     6601 gatcacacgc attaacggcc accccttgta tccttcgatt ataaaatcgc ggtgatagga
     6661 gccatcatca ctggaagagt cttcacactc ataatgtgcg accgtggcag aaggcggggt
     6721 tccttcctta caattggaca ttccacttgg catccataac gggttgataa gaataaagag
     6781 ggcaaatggg aatataaaag aaacgatcaa cccgaccagc aggttgacac caagcagttt
     6841 tttctgcagc ccaagtaaaa tggcaacagg caggctcagg gcacagatcg gcaaatacag
     6901 cagggaggca aatccctggt atgggggagc agggacgatt ttgaagacag agagcagagc
     6961 ggaaaagatc ggcgctgcca tgatgatcaa catgggtttg ataacgtcgg atttcatgga
     7021 gaaggttcct tatctgtttt tgaagtatgt gctcacaaga tcggaataat ccatatcggt
     7081 gtgctgatcg aggatgcgga agaagtcatc gcctgttacg attttgccgc gcccctggtt
     7141 gtagtaatcc tgtaaaaagg caaagaaggc tgcgtcgccg atgcgtccgc gcaggtcgcg
     7201 cataaagtga gcgccgttga aatagacaat gtatttgtag gtatcagcat cctgcccttc
     7261 ataaacgggg atgtcaatat cgccaaaagg ctggaagaaa tctatgcgat tcgtccacca
     7321 ccaggccaga gactctggat gtgcggcttc atagtagatg gcttccgagt aggtggtgag
     7381 cgattcatcc agccagggtt cgcgggcctg gtcatttgcg acctgatcga accaccattg
     7441 atgtcccgtt tcatgtgcgg caaccgagac gaggtaactt tgcggggttc catcatacag
     7501 gttgtagaaa ctgcggctgt ggaaatacaa cgcggaaaat tccatgctgt cttgaaaatc
     7561 ggcaaccacc acggaaaggg tcttgtgtgg atacggtccg aacatctgcg aatagacctg
     7621 aatggcctgt gcgctggtca acatggcggc ttcgcctgca acctggtaaa tgggaaggtg
     7681 ataactgtac acaaccgttt caccgacctg cgtgctggaa actagaaaat cgcggctggc
     7741 cgatatcgcg aaggcacgtc cgccggtaag ggtgtagcgt gtgaattcgc cgttcggttc
     7801 ggcgggtccg ctggcggcaa tgattggggc agtgtctggg ttggcaaatt tcaggttcac
     7861 ttcgaaatca gccatgggat acacgaggta ttcgccatgt gaccacggtt cacggatgat
     7921 ccattcacca tccttgaagg gcacaacgaa tggataccaa ttgacaaggt tcatctggag
     7981 gtcgctgtag ccgaagatgc gtgcgcgctg gctgttgacc tggtccatga acgggaggaa
     8041 gatgctgtag cgcagggaaa tcgtcgaaac agaatcaggc tcaagcgggg tgggaagcgg
     8101 aatgtcgagt ctgtgcaggt tgaaggagta attcgtgacg acattcccat ccacttgtaa
     8161 ttccatcaga taaaagcaat tcggccagaa atttggaggc acagccaggg tcaatgtggt
     8221 gagctgctgg cctgtgtgat tggggtagat gatgtcttga tccacgtcaa taaaatgttg
     8281 atcgtaatca aggaaggtgt tgatgatata tttttcccgc tccgggattg gaacgggggt
     8341 attggttgcc gtggggctct cagtggctgg cgggatgggt gtggaggttt caacaggcaa
     8401 tgtagccatg attggttgtg ccgtggatga cgaacatgcc gtgagaagca ggacaaggag
     8461 aagaatgata tggcgcatgg aactatttta accttttgtg tataatccag tcatcgcctc
     8521 gatagttcaa cggatagaac aaggcactcc taaggcttag atgtaggttc gattcctact
     8581 cggggcacaa cattaaggaa actctaataa aaaagtcttg ccaaaaaata gaaccgcgat
     8641 tattattacc aacgatgagc gccagggtgc ccccctcacc ctggcgctca tcatttaatt
     8701 ttataaacaa aaaaacatcc cacaagtttt gactttcgga aatccgaacc aaacttgcgg
     8761 gatgttttcg tttttatgat cgaattactt cttcggagtt ttgaccatct gccgcgccgt
     8821 ttgcagcaat tcatgtgcag aacgcagcgt tccttcgccg acttcaccaa gcatttgcga
     8881 aagttcaagc aggcgcggtt cgccttcgag tctttccaca cgggtgaggg tacgaccctg
     8941 gtccaccagt ttttgaacct gataatgctg gtcgccaaaa acggcaagct gtggaaggtg
     9001 tgtaacacaa aagacctgat gcgagcgcga aagattccat agtttttgac caaccaccat
     9061 gcccacccgg ccgccaatgc cctggtcaat ttcatcaaag atgagagagg gcacctggtc
     9121 ggcgcgagcc agtacatttt tcaaaccaag catcaaacgg gaggtctcac cgcctgaggc
     9181 gatcttggca agaggcttga gtccttcacc ggggttgggt gcgatcagga actctacgcg
     9241 atcgaaacca ttttggtcga aagcgatgcg cgtgccatca gcaaggggaa tgccgttgac
     9301 atcaggcttg gtctgaaaat ccacgctgaa acgggcggct tccattcgga gatcacccaa
     9361 ttcggcttcg atgcctttgc tcattgtcaa tgcggcggat ttgcgttttt cagaaagcac
     9421 accgcccaat ttggccagtt tatcgagcag tttcgcttcc tgcatttcca attcattgat
     9481 gcggtcggcg gcatttgtaa gggtttcaag ttgtttgcgg gcatcggctc cataagccag
     9541 cacagatgaa atactgccgc catacttgcg cgtgagagag tggatcaggt tgagtcgttc
     9601 ctccacttca tcaagtcgtt tcggattaaa ttcgatctct tccagataat tctgcaaccc
     9661 atgcacaaca tcggaaatcg tatcgagcat caactcggcc tggttcgaaa gctccacttg
     9721 ttcaggatct atttttgcga gggcggccag cgcctgcgca gcctgaccga tcaggtcggt
     9781 tgctgcgggc atatcggggg agccttcatc aagaatcgcc aatgcttcct gtgcattttg
     9841 cgcaagcgat tcagcgttgg caaggcggtc acgttccttg cgcaactctt cgtcttcacc
     9901 cgctttcagg cgggcgtctt cgatttcttc agcctgatag gtcagcatct cggtgcggcg
     9961 ctcagaatct gattgcgcct tgcgcagctc caccagttca cgtcgcagcc caagcaaagc
    10021 ctgatagctc tggcggtaat cgccgagggg ctttacaact tcggcgtagc gatcgagcag
    10081 gccaaggtgt gcgcgtggat cgagcaggga aagatgttcg gcctgcccgt ggatgtcgat
    10141 cagcagggcg cccagttcct tcaacaggcc cacattgact gtgcggccat tcacacgcgc
    10201 cacactgcgc ccctccttgc ggacttcgcg catcagcacc acataatttg gatcgtcgag
    10261 caattcttcg cgagtcagga cctcggtcac cgcttctttt tctggacctg aaagctggaa
    10321 caccgcttca acaaatgcgg cgtctgaatc ggagcgcacg aaggtcgcat cggccttgcc
    10381 gccgatgagc atcacaactg catcaaggat gatggatttc cccgcacccg tttcgcctgt
    10441 gaggatgata agtcccttgt caaagcgcag gtcaagttta tcgataatgg caaagttctg
    10501 aatatggagt tcggtaagca taagttatcg tctaaagacc aaaccagaga attgggaata
    10561 cgccaccggc gcagcgatga caaggtaaac gatctgccag agtactggcg aaaaagatac
    10621 aactgacccc atgacattgc cgccaccgcc aagcaaggtc agcaagccgg gcaggccgct
    10681 caacaacaaa acaggcagtg cgccaaggat catgcccacc accagtgtgg tattcaacat
    10741 gggagagcga tggtttttca caaaacgccg tgtgacattg gcgatcatcg tgcctgccag
    10801 tggagccaga ccaatgatga aaaagcccca aataatggat gtgatgatga tggtggcgac
    10861 cgccgccagc cctgaaagaa tcgcagaggt gaagaacacg gtcaggtagt cataccagac
    10921 cgccgtgtca aagatctttt gatgctggtt cacacaatcc ttgcacatgt acccggtagg
    10981 tgtacgttgg gcgcaggagg cacaaatggg tttttcacaa cgcttgcagc gcaggctggt
    11041 ctcacgcccg gggtgggcat aacaataggt ggcagttggg gtgatctctg tatcggtcat
    11101 cgtggaaatc ctaacgagtt ttcgttcata tgggcagtga ggttgcggta gaaatacccc
    11161 gggtctccaa agcgaacgaa ctgcgcggtg acatctgcag cggttacttt tacctggtca
    11221 tcctccatca aaggcgttgg gggttgacca tccacactta gaactgaatt ttcacttttt
    11281 acaacaatgc taacggaaga accttccgat aaaaccacgg cacggtccac tgagaggtgc
    11341 ggggcaatgg gcacgagcaa aatattgcgc aactccggcg gcaggatggg cccgccagcg
    11401 gcaagtgcat atgcggttga gcctgtcgct gtggatgcga tcaaaccatc ggcaacataa
    11461 ctggtaagtc ggcgattatc caccgaagcg gtcaggcgca ccgggcgcag gttctgccca
    11521 cgggcgacga caacctcatt caaagcattg gagcggttga tgctttcacc cgcacggata
    11581 tgctcggccc gcaacatcat gcgattttcg atccatgctt cgccttcgaa caacttttca
    11641 aagtactcac gccattcctt gcggtcgatc tgaatcaaaa agccaagccg cccaaggttt
    11701 acaccgagaa tgggtacgcc gcagggcgcg cataaatgcc ccgcccgcaa gacacttcca
    11761 tctccaccaa cggcgatgag catgtcgaat tcgccatttt tgacacgcct gcgcaattct
    11821 tcatcataca aggaaccaaa cggcgcttga atgcctctct ctttgagaaa ggacaccatg
    11881 gcttccgctt cggtgaatgc ttccggcatt ttggggtacg ccgtgacaac aggatttttt
    11941 ggaaggaagg gatcgggcat aacgaaatta tacctttgga atgtctcagc gcatccccgc
    12001 cttcaggcaa ggctttgatc acacatattc ctgaaaccac atttggcaca gcgggcggcc
    12061 tgttcatgtg aacgcggaac gtcattcttg tgttcatcga gtttcatgtc agccagaaga
    12121 tcgatcaggg cggtttccaa ttcatgggtg taatctacgg cgaaatcacg attctcatag
    12181 tggatgatcc catagggcgg acgttttccg taagtctttt ccaccaacag gcagtaggca
    12241 gccaattggt agatgtgaga gtcgtaaggc gcctgcgggg cacgccccga tttcacttca
    12301 acaggaatga tctgtccgtt cttttcaaca aggtaatcag gcttgccagt caactcaagt
    12361 gcgggataaa aaagcggctt ttccactttg ccccatccac gtgtatctgt gtagatgatg
    12421 cggcctccag gcagccctgc ttccttccgc tgaaccgatg atttgcggaa aaagaaaagt
    12481 gcaaagagga agagagccag ggcaagataa agcatgggaa ggataagggg cggttagatg
    12541 ttctcggcga tacgaataat gcgacccaga cgggtaaacg ccgccccgga aaggtcatcg
    12601 tttctgcggt tgagggtttt aatgaaatcg tccctgttcg attcattgac ggcgggcagg
    12661 gttcgttcag cgtgttgggg tacttccccg ggacgacagg ctgagaggtg tttcaataaa
    12721 tatgggaaaa tggctttgtt gtactcttca tttgcggcgg cagtttgcgc caggaccgaa
    12781 atggaattat ccacggtaat gaccgaacca ttctctttgg ctttcttgat ctcatccaaa
    12841 tgcttgaaga tgacttcggg ttttactttg gctacttcag cgagtgccgt catggcaccc
    12901 cagaccatgt tgttgtgttt gcttttcagt aatttgacaa aatcttcggc gtaaggggcg
    12961 atgagagcag ggtcaattgc accgatctca tacatcacgt ggatgcaatc ccagtgaacg
    13021 tttttgtttg cgctccacat gttttgggcg atttctcgaa tccctgcctt gtcgttccgc
    13081 gctgcaagat cgcgtgcaag gtcaaggttt ggggtgcgat cgcggcgggt ttgcaggtgg
    13141 gcaaggcggc ttaaaacagc ggacatgaaa tggattctcc aagcaattta taactgggcg
    13201 gtgcaataag ccaccagcag gatcaaagcc cctaaaagca gaatcaggcc aacggtccgg
    13261 gaaagagacg aggcaagggc ttgacgacca tgcctagaat ggagttcagt ccctgcggcc
    13321 aattcagctt tatttgcaga ttcgaatccc tgtttgcgat accaccagga gcggcggcaa
    13381 tacaggtagt ttccaatatc ggaagcacgg atgatgggca tggccagagt atagcacaaa
    13441 ttttctatgg ccattcattt cattttcaat atcgagttat gataaacttc gcgggtaaat
    13501 tacctttcat atcagaggaa cactcatgac caaacatcaa gtccacccta ttgagattgc
    13561 ggggatcaag cgtgaattgc ccctttttga ggtaaagcct ggtttgcgca ttgccatttt
    13621 gaacatcctt ggcgatactg aattggtgca ggcctgtgcg cgggaacttg gaaagaaact
    13681 tcaggcggtt gattacgata tccttgtcac tgcggaggcc aaatcgattc cgctggcata
    13741 tgcgttgtct gttgaaacga agaaaccgta tgtcatttta cgcaagacct acaagcctta
    13801 catgggcgca gcactcgaag ctgaaacact ttcaattacc accggccagc cgcaggtttt
    13861 gattctggat gaaaaagacc gctccatcat tgcgggaaag aaggtcgtga tcctggatga
    13921 tgtcatcagt acgggttcca ccctgcaagg gatgcgcatg atcctggata agactgatgc
    13981 aaaggtggtg gctgaagccg ccatccttac cgaaggtgac cgtgcccaat ggatgcacat
    14041 catctcgctg gggcatttgc cgctgttcac tgggtaagtt tgattcattc aaaaaaacgg
    14101 cactgggaag ttttcccagt gccgtttttg tttaagtgtt catttcggtt tcaaatcccc
    14161 tgcaatcaac cttgcttttt gttcgcgctt caggcgtttg atggcaagtt cgcgcttcat
    14221 ggctgcggtg cggtctggct gtatttcgag gtaaaccagt ttcacagggc ggcgggtgct
    14281 tgtatatttt gcgccaatgc ctttgttatg ttgggatacg cgccgctcag ggtcggtggt
    14341 ccagccagtg tagtaagttc catcagaaca ttctacgatg tagcaatagc aggtcatttc
    14401 cggttgtgtt ttttcacgaa catgccgcca aagaatcgct cggaaaacgt gggcatatca
    14461 gccgcttcag agcgggtagc acccgaccaa tcagcagcga ctctttcact gagccagcgc
    14521 tgacggcctt ccaaagccgc ctcaagccgc gccgccacac cctcatcatc ttcattttca
    14581 atatcctcac gaaaaccacg caacgccgcg atcatcacat caagtgcatg gatcatccct
    14641 gctcgatttt gcagggagga aagacgcaaa gaatcgatct catcctgata agccaaccca
    14701 gcggttactg tggcaaaggg gcgccccgcc actttgcgtg cttcctgcca gccgggctga
    14761 tccactgtgg cgttcaaaag tgaagcagcc acgatctggg gcaaaaggtg cgtggtggac
    14821 atcaatccat ccgattcggc tgggtcggtg agcatgggca acgcctccaa caagcggacg
    14881 aaatccactg cggtcgtcac tgccgattcg ggcgttccgt agggtgcatc aatgaggaaa
    14941 aggccattcg caaacagatc aggcttggcc gcagcctgac cgaactgaaa atcatggatg
    15001 taaacagggt tgatcgcggg caccaatccc acgtagtggc ggtctgccgg cagaatttcc
    15061 tttgcccatt tggctacatt ggctttgatg ggggaggtat ccaaaaccac tgtcccctct
    15121 ttcaaatccg gcgcaataaa ttcgagggta tcacgcacct gactgacagg cactgtcaac
    15181 accacgatcc gtgcatcgcg aacggcggtg ggcaggttat gctccgcctt atcgatcgca
    15241 tccttttgct gggcatcacg ctccaactcc gcctttttat cgtgccccac ccgcacaatg
    15301 gaggctttat gggcggcaag cgccattccc atcgaagtac cgatctgacc aaggccgata
    15361 attgtgattt gtgtgggcat aatatatacc tcgcaggagt ttgataaaac cattatactg
    15421 ccactatctc gttaaatacg agaaccgcgg acgccgaccg cggttctctt ggtaaaggag
    15481 gagaagagaa atcaccttac gaacgtaaat ttatcatgct tgtccaaaaa ctacttgacg
    15541 cctgacctac gcttacccta cgattgtttg acaaattaca ggaatgcaaa cattctcaat
    15601 atttttcgga ggacaacgtg atgacaaacc tgcctctcaa aatttatctt ggcgaattga
    15661 atcacacacc tctcggggat ttgcgcatcg cggcctccga cctgggtctg gttgctgttg
    15721 aatgggcaga gtctcagccc aggctgtttg aatacctctt ccgcctgaaa gggaccgtgg
    15781 agcacagcca gaaagcaacc caccgctaca ccaaagaact tggggaatac ctgaaaggca
    15841 aaaggcatga tttcaccttc gaaattaact ggggaacctt gaaacccttt cagcgcaaag
    15901 ccttgaaagc cgtttatcag atcccctacg gcgaaacgct cacctatgcc gatattgcca
    15961 tgcagatagg tcatccaaaa gccagccgcg ccgtggggcg cgccaatgcc accaatccca
    16021 tgccgcttgt cattccctgc caccgtgtca tcggcaagga tggaaaactc cacggctatg
    16081 gcggcgggga tggaatcccc accaaggagt ggctgttgaa actcgaaggt gcggtcatcg
    16141 cctgatatga tacacttcgg aaaattcctt actttaatat ttcagctaaa gcccaatcac
    16201 cttgaacatc ctcaccgaac ttttctggac attcatcaaa gtcaacctgc ttagcacctc
    16261 cggcccagcc tccgtagggt tgctctatca cgaagctgtg gggcgtttcg ttacagaaag
    16321 ccaatttgta caagctgtgg ggttttcctc ggttctgccc ggcagtgatg ctttgcagct
    16381 tgccatgtat attggctacg ctgccggggg aataccggga ggtctggtgg cattgcttgc
    16441 atccatattg ccacccaccc tcatcattct gggggtaaca atgatcctcc accgtttgag
    16501 acgcgaggct tgggttggtc attttgtaga aggtctcgcc ccggccatat ctgtgctcat
    16561 ggtcttcact gcgtggaaga tatttgaaaa gggtggtgaa actggcccac tgggctgggc
    16621 gatcggcatc cttagcttga tcgccatgtg gttgaaagcg ccagagcgta ttgtcgtcat
    16681 ccttgccggc atagccggaa tcattctcct ctcgccatga aacaatcaaa aattaccgtt
    16741 tggagcagcc tgttatggat gttccttaaa gtaaatcttc tttccccttc agggcctgct
    16801 tcggttggcc ttttatacaa ggaagcggtc ggcaagatca tgaccgaaga acaatttgtc
    16861 gaggctgttg gtttttccaa tgtattgccc ggcagtgagg ctctaaaact tgccatgttc
    16921 gttggctttg cagcaggcgg tgtgcctggt gtgttgacag ccctgcttgg cgcggtgttg
    16981 ccccccactg tgatgatgct ggccgttgcc tttgtactac aaaaatttca gcaggaagat
    17041 tggcttcagg gatttgtaaa aggtatgagt ccggccatcg ccgcacttat cattgtcgtg
    17101 gcgtgggaac tcttccatgt tggtcaaacc aagaaaattg acaggcgcac tttattcatt
    17161 gcggctgtca gcgccgtagc tttatggttc gatctgccct ctccgctggt tttactaggt
    17221 gcaggcgttc tgggcgtatt cttatttcgg tgaatggaaa atatatgacc caaaatgtat
    17281 taatcgccac caatggattt aaaggcacct ggccttccat cgaatatggg acatggctcg
    17341 cctcgacact taacacgaac gtcacattgc tcggtgtaaa tgagaagcca aacccagccg
    17401 ccattgacga tcacaaccca ttggaagatg tgttcgaagc cgctgtggaa ttgttccaga
    17461 aaaacggcgt gaaatatgag ctgcttgtgg aaaatggaaa tgccgaggat gtcattccgc
    17521 aatgggcaaa gcagagagat tccatcgttg tacttggccc gctgggacgt ccgcagatcc
    17581 gccgtctgtt gacggggcga tcgatccgtc acttgatgga ggaaatcgaa cagccgatcc
    17641 tgtatgttcc ggaagccacc cttcctctga aaaagattct gatctgcatt ggcggccttg
    17701 gctacgaagt gactgcagaa catattgcca tgcaaatggc gatgaagagc cgcgccgaag
    17761 tcaccctgct gcacatcgtc ccgcccatgg atctggacta ccccacggca cgtattgtcc
    17821 gcgaaaattg gcaaaacctg accgagaccg atacgcccgt tgggcgaagt ctgcgcgagg
    17881 ctttacaggt ggccaaagac gaaggcctga ccgccagtgt aaaggcgcgt cagggaaatg
    17941 tggttgaaga aatattggca gaagtaaaag agggtggata cgacctgcta tgcatggggt
    18001 cgccatacag cacaaatgca ctgcgccagc tttacgcccc taatgtgacg gctgagatcg
    18061 ctgaagccgt tacctgcccg gtcttatcgg cgcgatacaa gagggaatag aacttttcta
    18121 tctggccccg gggacgtatt tatcttccca ctccgcaatg gcggcgcgga tttccctttt
    18181 gatcacagca tcacggacat catccactgt ggggaatgtt tcgattccca caatgacttc
    18241 cacaccgcct tcgggggatt cttgcaggcg aattccagcc ttttccagcg gtgttccgac
    18301 caaacgggct tgcagcacgg tatcaatttg attgacaatg ctcatcgccc ggatggattt
    18361 atccaactcg gcctgggcag ctaccgtaac aatgggaggc atgggacgag gtgatgtcgc
    18421 ttgaacagga gcaggcgtgg gcgcaggttg aaccggagcg gcgggtctct cagaagccgg
    18481 ggcagggctg ttatcaagcc agggacgaaa gatggtgatg agttcgatca ggcgcttttt
    18541 ttcttcggaa gagggtggct tgttgacaac tctgccgtcc atttcaagtg tgtagtgggc
    18601 attctctttt ttgagacgca gcaagccggg gtcatccttt ttttgtgaga cgctttcctc
    18661 ttgagaagca aggatcattt tgttttccgc ttccctgatc ttggcttcgg cttgaacttc
    18721 tgcggcagtg atctttttat tcgtacggat attggaatca agaaacccga taaaaaaacc
    18781 gatcagcaaa accacaagac ccactcccca aacggctggt acgggccagg taacggttgc
    18841 aaattggagc tccatgattc atttcctttc gattgcctgg cacttcgggc aaacatgcgt
    18901 gccacgctgg ccgacggtca acttttgtat tgtggtgccg caaacaggac agggcttgcc
    18961 atcctgatcg tacacgcgaa aatggttttg aaaatcaccg ccgcgataca cccagtcaat
    19021 gctggcgccg ttgcggcgga tgccttcctt aagcacggca cgaatcgctt tgtgcagggc
    19081 ctgcgccttc tcttgtgata cggtattcga taaggcctgc ggatgaagtt ttgcaatatg
    19141 caggcattca tccgtgtaaa tattgccgag ccctgcaata aaagattgat cgagaagcag
    19201 gggctttaat tgtcgtttac gcgtatgcag gttttcgaaa agccactgcg ctgtaaaatc
    19261 atttccaagt ggttcagggc ccaatttgcc gagaattttt tcaggctggt ctgtcagcca
    19321 cacgcgccca aatttgcggg tatcgttgaa agcgagggcg gtgtgattgg aaagatgcaa
    19381 aagcaggcgg tcgtgttttt caggcttaat tttacctgtt cttacgatca aatctccact
    19441 catgcgcaaa tgtatcagga gattgtaatc tttcaggtga acaataaggt attttgcccg
    19501 gcgcgaaaca tccacgatcg tttgcccgat gatccgctgt ttgaactgtg atggagaagg
    19561 ggttgccagc gtgcgcgccc agcgtaaatc agccgagagg atggtgttcc caaccaactc
    19621 gggttttagc gaacgggcaa aggtttccac ttcgggcagt tcgggcatga gaaatctcag
    19681 gcgggaattt tcaaaagaac cgttttactc gttgaacatc tcaccaaccg aacggccttc
    19741 atgcgcacgg cgaatcacct caccgagcaa ttgcgctact gaaaggacgg ttgtgcggct
    19801 ttcgaggaat ttccattttt cgggagtgag cggcgccgaa tcagttgtga tgatgtgttt
    19861 gatgggcagt gaagccagac gttctgcgcc gttgcgggaa aggatggcat gaacaaatgc
    19921 cagatagaca tcgcgcgcgc cattttgctt caccacatta acggcctgag agatggaacc
    19981 gccggtatcc acttcatcat ccacgatgat gacatcacgg tctttcacat caccgatcaa
    20041 ggtcaacgct tcggctttgg catcgttgcc gtggcggcgc ttttctatga aagcgatcgg
    20101 catgtccata tcagcggcgt aattacggcc cttcttggca aaccccaggt ccacagaaac
    20161 caccacgggg tctttcattt gggagcgcat attgttgttg atgtgatcca ccagcaggtg
    20221 agatgcagtc aacacatcac cggggatgga gaagaacccc tgtacctgcc cggcgtgcgg
    20281 gtcgagggtc atgtaacggt cggccccggc cacttcgagc atatcggcaa ccaggcgtgc
    20341 cgtgatcggc acacgcggct ggtccttttt atcggagcgt ccataacaca agtatggaat
    20401 caccgccgtg atccgcgccg cagaatcaag ccgcagggtt tggatcatga tgagcaattc
    20461 catcaaattg cgatgtaccg gtgaggacgt ggtctggatc acgtacacat cctgtccacg
    20521 cacactgccg ttcaacttca caaacaggtt ctcatttggg aattcgatca cctcacggct
    20581 gctgacaggc tggtcgaggt aatctgcgat cttctgggca aggtctggcg agcctgtgcc
    20641 cgcaaacaac ttgatccctc catacacttt caggtcacga tgaacatgat gcatctattc
    20701 tttgcctttc ttcgcgttcc attctgaacg taaaatactc atgacgaaca catcgtcata
    20761 ttttccatgt ttgtaaaccg cctcacgcag gcggccttct tccacaaacc ccgccttttc
    20821 ataggcatgt tttccgcgct cgttttcggc atacacctgt aaaaagacgc ggttgaaatt
    20881 cagggtttca aaacaatgct gcgcgagcag cgcgatcgtc tccgttccat agcccttgtt
    20941 ccactcgctt ttttcgccga tcataatgcc gagctcacca caacgggaaa cagagtccac
    21001 tgcaaacacg ccgcaattgc cgatcatgcg ccagccttcg ccctcacgaa tctcaatgac
    21061 gaggaccttc tcattctgcg gtcgctggtt catggaattg aaccagtttt cttcatccac
    21121 catggacatg ggcaggctga gcaccagatg tcgggtcact tcagggtcgt tcacccattt
    21181 atggaaggtc gccacatctt cccgttcggc agcacgcagg cgaatccgtt ttccgtagat
    21241 gatgctcatc aggcatctcc gtccatcaaa gatttcactg cctcccaggg agaaattttt
    21301 ctctggaaga ttctctccaa cgcgttttcg tactggcctt gtgagacctt ttcacgaaaa
    21361 cggctgacca gtgtctcctg aatcaacgca tccaactcga cctcaagcct ggctcgctca
    21421 cgaacggccc aatcgccgct ttgggttaag tgtgccacat gccttgcgat ggcatcggtc
    21481 aattcaacaa tgcctttgcc ttcggtggaa accgtccgtt gaatgggtgg aatccatatc
    21541 ggcgctgatg aggcttcttg ttttgaatca ggcccacccg aagttggacc ggatgaaatc
    21601 cgcatggctg tgccgtgatg ttgaaaaatc cgctcggtgg ggtgggccag atcaagcatg
    21661 gatttcaaag ccttttcggt gttttccacg ccgggtcgat ctgccttgtt gacgacaaga
    21721 atatcggcaa tttcgagaat gcccgctttg atggcttgaa tatcatcgcc aaggccgggg
    21781 gcttcgacca ccagtgtggt gtgggcgagc cgggcaacat ccacttcgct ttgacctgcg
    21841 ccgacggttt caatgatgat gacttcataa ccagcggcat caaagacctg tgccatgttc
    21901 gcggtggatt gcgcgagacc gccgagcgat ccacgggtgg ccatggagcg aatgaaaacg
    21961 ccggggtcac cggagagatc gcgcatgcgc acgcggtcac caaggaccgc gccgcccgtg
    22021 aacgggctgg atggatccac ggcaatgaca gccacccgct tctgcgccgt tttgcgataa
    22081 tgcagcgcca gttgattcac cagcgaagat ttgcccgtgc cgggggaacc cgttacgcca
    22141 atgaggtggg ccttgccggt atgagggaat aattcgatca atgcggcacg gctttcaggc
    22201 gcgtcatttt ccacctgtgt gagcaggcgc gagagcgcga gtcgttcgcc gttgaggact
    22261 gcctgtgagt aggtcatgga gtggtttcga aataagtgaa gagatgatcg acacaatcaa
    22321 agatgaacgg ggatgaaaac ctttcagtat ctccgttcat cttgggtttg tttcagcgaa
    22381 aaaatacaca gagcctttgc tctgtgtatg tgtgaacagg ttaccctgcc accgccagtt
    22441 tgacatcccg gataatgtct tcagtggatg cgccggggcc ataaacgcct gttacgccca
    22501 tttcttttaa gcgatgcagg tcttcatcgg ggatgatgcc tccgacgaag atctttacgt
    22561 gagactggcc gttcattttc aacagttcca acaaacgcgg tgtgagtgcc atgtgggcac
    22621 cggaaaggat ggagaggcca accacatcca catcttcttg aagagcggct tcggcgatca
    22681 tttccggggt ttggcgcagg ccggtgtaga tcacttccat accggcatcg cgcagggcgc
    22741 gcgccaccac ttttgcgccg cggtcatgtc catccagacc gggcttggca accaacacac
    22801 ggattttctt ttcggtcata gttcctccga gggaatgatg agtgaaatta tactatgtat
    22861 cacaagcgca gttattaggg agtggctaat ttcgtgggct tgccaaccaa agatttaacc
    22921 acagagcgca cagagaacac ggagaaaacc ttttgaaaat ctctgtgaac tccgtgatct
    22981 ctgtggtgga agagtcgtta gcccttcctt ttagccactc ccagttatta cctgatgaca
    23041 ggcagtgcct gatgagttgc ccgatatcag gcaataaaag agcgacattg aatgtcgctc
    23101 tcgttctgtt ctacattccg tcatcctttg ggggtgcggt ttgaccataa tcaagcacgg
    23161 ctcgcatttg ggtggcacct tcgggcgggc cgacaatctt tttatcttcc attgtgtcga
    23221 cgatgcggga ggcgcgggta tagccgatgc gcagcctgcg ctgcagcatg gatacagatg
    23281 cgcggccttc cttgcggacg atttcaacgg catcatcgaa aagcgggtcg ccttcaactt
    23341 tggtcatatc ttcccaaagg ggtgcctgtt tgagcggtac gttggtcggc actgcatcgg
    23401 cagggttcga accagccaca gcatacggac tcgccccgcc agcctgattg cgccagtatt
    23461 ccaccaggct ttgaatttca tgatctgaaa catagacgcc ttgtaaacgt gcggcagagg
    23521 gagcatcagg cgcctgatag agcatgtcac cacggccgag caagcgttca gcgccgggct
    23581 ggtcgaggat cacacggctg tcagtattgg aggccacggc aaaagcaatg cgggccggga
    23641 agttggcttt gatgagacct gtcaccacat ctacagacgg acgttgggtg gcgagaatca
    23701 tatgaatgcc tgtggcacgg gccaattgtg cgaggcgcgt gatggtcttt tcggtctcgt
    23761 caggagcgat catcatcagg tcggctagtt cgtcaatgat aaccaccagg aagggtagtt
    23821 tcttttcgcc ttttaatttc attttggcgt tgtaatcggt gatgttgcga gaacccacct
    23881 gcgcgaacat gtgatagcgc ttgtccattt cgcgtgtcat ccattgcaag gctccgatca
    23941 cgcgatccat ttcaacgacg accgggccaa gcaggtgcgg aatgccgttg tagccggtca
    24001 actccacgcg tttggggtcc accaatacga ggcgcaggtc atcgggggtg ttgtagagca
    24061 acatgcaggt gaggatggaa tttacacaga ccgatttacc cgagccggtg gtgcctgcga
    24121 tcaacaagtg cggcatgctc tcaatactgg caatgaccgg cagaccagcc acatctcgtc
    24181 cgagtgcaaa gtttaggggt ttggtgttgc gtttgtatac ttcgccttcg agaatatccc
    24241 gcagggcgac cattgccatt tcttcgttgg gaacttcaat gccgacataa ctgtgaccag
    24301 gcaccggcgc ttgaatgcgg atgcgagggg cggccagcgc aagggccagg tcatcggaaa
    24361 gggaggcgat cttgctgaca cgcaccttgg tctttacccc gcctctggac tcaaggaaaa
    24421 gcggttccac gccgaattgg gtgatcgaag gtccgcggct gatggcgacc acctgtgccg
    24481 gtgcgccaaa agaggcgagt gtttcttcga tcaatcttgc gcgttgttga atgaaatctt
    24541 cattaatgga gggcgcgttg cctgcatcaa gaatatcgct gacgtttggc agagtccaat
    24601 ggatgacggt ggcgccgctg gtggtacggg tgggttggcc aatccccgcg gcgggtttca
    24661 catcgggcat ggcggagggg tctatcgggg tgaggtcttc tgtcgaagca gaagcaggcg
    24721 cgggtgagtc ggaggcgatg ggtttgttca gcaggttccg aatccaggtg aagatcggtc
    24781 cgagccagaa gaataaatcc tgtacgggtt tatcaagcaa gacggcaatg ccaatgacca
    24841 gccaggcaac aagtgcaatg aaggcgccgc caccgccaaa cccaacccaa agaaaccatt
    24901 ggagttttcc accgatgtat ccaccaccga tacctttgag ggcttcggct tcagcggttt
    24961 ctgctgtggc gacgaaagta tgcagaacga tgagcagcca taaaaagagg atgacgctgc
    25021 ccaccacacg ttcaattgaa agtggaggga tgcgttcgat cttgcggaag accagccaca
    25081 gaccaaagat gagcaggcca agtggaagga tgtagactcc ccagccgaaa atttgggcaa
    25141 tgaaaaaaat gatgccacca acaaccgctg agcgattggc agaggcaagc cccagaatga
    25201 tgatcacgcc aaagaaagcg aggatgatcc caaccacatc cagtttgcgc tcgggggaaa
    25261 gcctgtccca tgcggagagg gcgggtgttg ggggcggggg tggtgtaggc gcaggagatt
    25321 gtcccttccc tttttgggcg gggtgattct gcggcgcttc cccttttttg ggaggaatgg
    25381 ttttgcccgc tggttttgcc gtcttcttgg gaggggtgga tggtttcagg agtggcatat
    25441 gaaaaagatg aaggagacag gatgaaggat gaaaatgatt cgcatccttt tttctgccca
    25501 acatcatatg atgtttatat ttgttgaagt taaaaaagat tatagcacaa atggtctgtt
    25561 atcttcgcag cacagcgact ggttgtgcgc tgcgctttca aatactatcg attttttctt
    25621 caggcgggag atggtctttt ctttaaggta caattgcggg gttttacgcc gtttctcatt
    25681 gcatctttcc gattcccgtt cccccgggtt ttcaagcatt tcctgaggaa ttcaccttgt
    25741 tcgaaatact ttttcttggc acatctgcct ccgcgccttc tgcaaaacgc ggactttcag
    25801 ggcagatcgt ttcccataat gaatatcgtt ttcttgttga ttgcggtgag ggcacgcaac
    25861 gacaaatcct tcaatcgggc atcggtttta aacgactgac ccgtattttg ctgactcatg
    25921 gccacctcga tcacatcctt gggctgggcg ggctgctttc cacttttttg cgctgggaag
    25981 ccattgagga acttgagatc tatgccgggc acagtacgct cgaacgtgtg cgcacacttt
    26041 tgtatgatgt ggtcttgcgc ggcaaccagc cgccgatgcc attgatcctt aaagagatca
    26101 aacccggggt cattttcgaa gccgaagatt tcacggtcac tgcgttttcg gtgacccacc
    26161 gcggcccgga ttgccttggg tatattttcg aagaaaagcc gcgccgtccc ttcctctcac
    26221 aaaaagcgga tgaactcgga gtcccatttg gaccggagcg ccgtgatctg gtggaaggca
    26281 gggagatcac cctcgccaac ggcacccgca tcaaacctga cgaggtgctg ggtgactggg
    26341 aaaagggcag taaacttgtc cttgtagggg atgctggaag aaccgacaat ctgctcgaag
    26401 tctgcaagga tgccgatgcc ctggtgatcg aatcgacata tctggatgaa gaagcagaca
    26461 tggcaaagca attttcccat ctaaccgcac gcatgggtgc ggaactcgct caaaaagcag
    26521 gggttaaaaa actaattctt acccatattt cgcgccgcta tcgtgaaaaa gatgtgatcg
    26581 ccgaagcgca atccattttt gccaatacgg tggtggcccg cgattttgac aattatcaaa
    26641 tcaggaaaga aacagaacat ggcagataaa tttacagtag gggtacttac ttccggaggc
    26701 gatgcccccg gcatgaatgc cgcgatccgc gccgtggtgc ggaccgcgtt aaccaataac
    26761 atgaaagtca tcggtgttca taatggatac gaaggcttga tcaatggcga gtatcatcaa
    26821 ctggatgcac gtgcagtcgg cgggatctta cagcgcggcg ggaccatttt gctcaccagc
    26881 cgcagcaaac ggtttcagga accgacaggc cagcgcgatg cgatccgcaa gatgaacgaa
    26941 gctggcatgg atgcattgat cgtgatcggc ggcgaaggct ccatgaacgg cgcgtatgcg
    27001 ctctcgcaaa aaggtgtgaa agtgatcggc atccccggca gtattgataa cgatatctgg
    27061 ggcacgaaca ttgccatcgg taccgacacc gccatgaaca ccatcatgga agccgtggac
    27121 aaattgcgtg ataccgcctc ctcccatcaa cgcgctttcg taatcgagac catggggcgt
    27181 aacagcggtt accttgccgt catggcggga atcgtttgcg gtgcagaaat ggtgttgatc
    27241 cccgaagtac cagtaaccgg tgaagaagtg gccgccgcag tggaagatgc ctatcaacgc
    27301 ggaaagacac acgcgatcat catcaatgcg gaagggtcag ccatccgcac cactgacctg
    27361 gtggaaaaga tcgataccct cgatctcggc ttcaaaaccc gcatgaccat tctcggccat
    27421 attcaacgcg gaggttcacc aaccgcctat gaccgtttgc tggcctcacg catgggtgtc
    27481 aaagctgtgg aggcgcttgt ggaaggtcaa catggcgtaa tgaccggact gaaaggcaaa
    27541 ggcgtggatt ttattccact tttggatgtg atcagcaaca agcgcaaggt caatatggag
    27601 tactatcaca tgaccaaggt gctggcgaga tagtgaaaat tgaagaatac gaaaaggccg
    27661 ttgagaagtt atctcaacgg ccttttcatt tatgaggctt tctgtaaagt tagcggctgc
    27721 ccagcgcttc ttccactcca tgcaggcgca tatcgagcag ctgctcaagg gtaaggtttt
    27781 ctccatatcg ctggcgcatt tcgcggataa agcgcggggt gacatggtga atgctcaatt
    27841 caaccaattc atcggcgggc aggttgctga aacccatttc gcgcagttca cgcacgaact
    27901 tcggggtgac gttgtgaatg cgcatttcga ccaattcctc ggtatccaca tctttcaaac
    27961 ccgcttcctt catctcagta agatagcgcg cagtgacgtt gtgaatgccc agctcgacca
    28021 catcatccac atcgaaaccc gttataccaa aatcacgcag ggccttcatg aattccggat
    28081 caatgttgtg gatgcttaat tggacaaggt catccgtatc cagattcttc aatccagatt
    28141 ctcgcatctg tgcaatgaat tcgggtgaaa caccatgtgt gcccaattca acgaggtcat
    28201 cgatatccag atctttgatt cccagtgttt gcagctctgc gatgaactca ggtgatatct
    28261 catggacgcc aagttccacc agatcatcca cgtcgaaatt cttgaagccc atcttcttta
    28321 actcggcaac atatttgggg gagatctcgt ggttactgag ctcaatcagg tcgtccacgt
    28381 caaaatcttt caaacccatg gcacgtaatt cggcgacata cctggcagaa acttcatgat
    28441 cgttcaactc gatgagttca tccacatcca ggtcttttaa tcccagttcg tgcatttcgg
    28501 caatgtattt ggctgagacc tcgtgattgc taagttcaac cagttcgtcc acatcaaggt
    28561 ctttcaagcc gacctcgcgt aactcagaaa catattttgc agatacatca tgaaggctga
    28621 gttcgatcag ttcatccata tccaggtcat caacacccat atcgcgcaac tcacggacgt
    28681 agcggggggt gatgtcatgc atgcggaggt tgatccactc gccaaggccg tctggttcaa
    28741 taccaagggc cagcatttca tgaacatact cggcagtaag gttatggtct tgcatttcct
    28801 gaatatcgct caaggtgaca tggtcaatgc ctgcagtttt gagtgcagcg accagttcgg
    28861 ttgaagcacg ctgtccgcgc ggggattggg gaggttcagg ccgaaggggc ggaaatggcc
    28921 ttgagggcgg accaggcatc gggaagtctg gttcggccga aggttcatcg ccctcctcca
    28981 acactttaaa aagttcctcg ccctcggcgg ctgtgatctt gccttcagcg atcatatcta
    29041 aaatcatcag gcgttctttg gttccgttca tgctgggtct cctttaattt gagcaagcag
    29101 tttggtggct tcctgcacgt tgatctcgcc acgactcagc cgtgcaagga tttcctgccg
    29161 tcgggtggat gggtcatctt ctttgggtgt ttcttcaaaa cccatttcag tgatgacttc
    29221 atcaagttgg cggcggatcg cccaatagga ttcgcctgtt tcgcgttcca tctctttgac
    29281 attgccgcgg ttgcgcacaa agatctctaa aaagtgcagg cgttccggcg agagacggga
    29341 gaatggcgaa agttcgaatt tgccgactac gccggtacca catgatgtac agttgagttg
    29401 ggtaaccacc agcggagacg aacaacttgg gcaggtttgg ggaattaatg gcataggggg
    29461 agctcctggc gatatattag caaatatttt tggatttgtc aatatttttg gtttacttca
    29521 aaatattttg gttttacgcc tatttatttt gttttacggc ttttgcgccg tttttttact
    29581 caaattaggg ttttacacgc caaaatgggc atccacgcag gaaagaccca cggtaaacaa
    29641 cgatttgtaa ccttgggaag gatctttcag gagactgaat gtccggattg attttgccat
    29701 ataatgaaag ctgttcgcct ttttctgaag acggcttgtc ttatggaata cccaccgatg
    29761 tcatcagaac ccaacgaata caatgccttg aatgaagcac ccgcccagga tactctaacc
    29821 ggcgcatata cacgtgcaac attcaaggca agtttacatg ccctcattga acaggcgcag
    29881 cgtgataaca caacatgttc tttaatggtg attgaccttg atcatttcaa aagcatcaat
    29941 gatgcatttg gccatgcaaa aggggacgag gtattaagtg gggccgctca tctcatcctg
    30001 caaaatacgc gggcgtccga tctttttttt cggtatggcg gtgatgaatt tgttttattg
    30061 ctgccaaaca caacaaagac agaagcgttc gatctggcac aacgcttact gggaatcata
    30121 cgtgcccagt cttttagcag ggatacagta ctgaccctga cattaagtat tggcgtagct
    30181 gccttccctg aagatgcatc ggacggtcaa tcgctatttg aaaaagcaga tgcgcgcaat
    30241 tacgaagcca agcggcgcgg acgggcatgt gtaataagca gggatggggg cacaacatct
    30301 gatttgtcat ttataaagcc atcgcgcctt atcgaacgtg acgccagtct ggaactggca
    30361 caaaactttt tatctgcctt agtgagtcaa caacacggcg tattcggcgt cattggtcca
    30421 aatggtgtgg gatgttcccg ttttctcgtt gaagtggaaa aacgggcgcg actgcaaggt
    30481 tttgaagtca ttgccctgcg cggaactccc caattaatgg ccaggcctta tggcgtactg
    30541 gaaaacatac tgcaaaaccc tgccggcgcc gcgttcactc aaaacaattt cgaaaaaaca
    30601 atcaaagcct ttcagcgccg actcaccgaa tcaggacgtg accgtttgct gatcagtgtg
    30661 gacgatctac cccagcttga ttggggcaca ttggacctcc tgcgccagat tctttcctca
    30721 aatgaattcc cggtgttggg attggtctac gccaccgacc cagaccatgc cagggtcacc
    30781 gcaccgtttc gggcggctct gtcagagtat gtgaccttgt ccccgctcac gcctgcaggt
    30841 ttgcaagtgt ggctgcggac attgcttcaa tgggacccgc ccaacgattt catccattgg
    30901 ctggaaaaac atacccatgg tttacccgct caagctgagc aggttttgaa acacatgttg
    30961 aaagagggcg cactaacaag gcaagattcg ggatggcatc tcagtgaaaa ctatgcaacc
    31021 atcggcgcag caggaatgcg gcaatggcaa aagcgtgtca gcccgaacaa tttaccggcc
    31081 gcactcacca gttttgtggg acgcgaaact gaactaaaag atgttcgtca actactggca
    31141 acgactcgac tcttaacggt ggttggcccg ggcggaatgg gcaaaacccg cctgacgctg
    31201 caagtggcga aagaagcaat aaatggtttt cggaatgggg tttggcttgt tgaacttgcc
    31261 cccgtcaccg accctgcact ggcgttgtac accattgcca cggtattggg agtggaacag
    31321 gtgcaatccc attctttaat ggagaccgta tgcacctggc tcagtgacaa ggaaattctc
    31381 ctcatcctcg ataattgtga acatctgatt gacatgtgcg cacaattttc ccatgaggta
    31441 ttgagcacca gtccgagtgt acgcattctt gcaagcagca gggaggcgct cgacattgca
    31501 ggagagctgg tatatcgcat gcctgcgttg gaaaccccaa atatacaagc cgaggttcct
    31561 gccggacaat taatgcagta cgctgcagcg cggctttttg cagagcgcgc cgcctttgcc
    31621 ctttccacgt tcagggtcaa tgcagaagaa gcgcctctca ttgcccaaat ttgcaggcaa
    31681 ctggatggga ttccgcttgc aattgagctg gccgccgcac ggttgagaac tttaacgctc
    31741 gaggaaattg ccacggggtt gaacgaccgt ttcaacctgt tgacgcgcgg caaccgcgca
    31801 gcagcgccgc gtcaacaaac cctgcgcgcc ctgatcgact ggagttatga cagcctctca
    31861 gaaaatgagc ggaggttatt ttgccgcctc tccatcttcc agggaagttg gactcaggat
    31921 gcaatcgggg tcatatgctc gggagatgga atcgaatcac acgacatttc aaatttattc
    31981 acccaactca tcaacaaatc actgatagaa gccatcgaac ggtccactga caaagaaccc
    32041 cgcttccgca tgctggaaac catccgtcag tatgcgtatg aaaaattatt ggaaacggaa
    32101 ggcagtgaaa ccatccgcca aaaacacctg gcctatttta tgggtttggt ggaacgagca
    32161 gcgccgaatt tacggacatt cgatatgctg atctggctgg atcggttgga agatgaattg
    32221 gacaatatcc gcgccgcact ggaatgggga ttgaaaagtg acattgaaac cgttttacga
    32281 ttggcaagtg cgttattgtg gttctggcat attcatagcc accaaaacga agccaccgac
    32341 tggctaaagc agggtcttgc acgcgaagca aacgaacgcg gcgggaatcc cataggtccg
    32401 cagcgtgcct tgttacgggg caacgcctta aatacgatca gtttcctaat gagaaactcc
    32461 gagcagcgga tcacattatt ggaagaatcc ctatcattgt tccgtgaatt gggcgtacaa
    32521 ggcaggcagg gacttgccac tgcactacgg atcctgggaa atagtgagtt ggaaaagggg
    32581 cacaacaaac aggcacgtgc atttgtggaa gagggcctgt ccatttttat ggagataaga
    32641 gaaccattcg acatggctga atgtctcgat ttattggtca gcctggcaat ggatgaaggc
    32701 gactttgaac gcgccagagg acttcaggaa gaagccctgt cattacgaaa agagatcggt
    32761 gacaaggatg gaaccgccta tgcactctac attttgggcc agattgctca cagacaaaac
    32821 aaagccgcac tggcacaggc cctttatgaa gaaagcctga aactattccg cgagatagat
    32881 aaccgaacat gggtggctga catcacaggc acctttgccg ttctggcttc agcgcaggga
    32941 aaccgcaaaa aaacaaatct cttgatcgaa gaaggtctgg ctctagccag agacctggat
    33001 gacaaatacg gactggctca cttaatgctg aatctgggag aagccatgcg ttttcaaggt
    33061 gaccttgacc gggcctcgca gctttatgaa gaagccctgg ttctaagcca aagaatggat
    33121 gccaacaact tcatttcatc cgtgctgtat ggcaaagccg aaatcgcccg ttctcaaaac
    33181 gatttagaac aagcatacgc cctgcactgt gaagcactga acatcaggca gaagatcagc
    33241 cacacatacg gaatcgtccg ctcgctgaac gcaattgccg aattgaccat ccttcaaggg
    33301 aattccccac aagccgttcg attatttggt gcgatcgaat ccatgtataa aacaagccgc
    33361 cctcctacag aacaagccgg gcatgaccgt gcggtctccg ctgcacgatc tcaaatgggc
    33421 gaaaccatgt tcaactcgat ctggagcgag gggcaaaaat tacctctgga tcaggcgatc
    33481 tctctcgtac tgacaaaacg atcagctact tgatcgtcac ctgaatcact gctgtatcca
    33541 cgatctgatc tgccctcacc accagcagct gcacagcata caaaccgctc aaaccgctgg
    33601 tatcccattc ggcaagcagg ccatcttcaa caggcgaaat cacatcgccg ccaagttgaa
    33661 cccattcctg cggattcaaa cccttcccaa cctgcacgcg ataatactga aaattctccc
    33721 cagccgcggt ccccatgatc ttcacaacgc cgccgacctc ggcaaacaat tttggttcca
    33781 caatattcgc gaacggattc accggcggcg cctgaatggc atcataagaa gcgggcggaa
    33841 tctccaaccc ggcgctcaag gcccagtcac gtgctgattc agggaccagc atgtaaatgc
    33901 gggtttcgat caattcgggc ggtgtaaata cggtggcaag cagacccgtt tcacggttga
    33961 tcccaaactc acggtacagg gtatcagcct gcacaggttc actgccattc agaaaaacct
    34021 cactcaccaa acgcggacat tcctttgtag gcagcatacc cgatgggtca cacactgtga
    34081 tggtcgaaac tccctgcggc gcggtccaac catcagcagg agcatcaccc gaagcaactt
    34141 tcatcaaggc gctccataac gcagcaggga agcgcggagt aaccgactcc gcttcaccat
    34201 gcaccccggt ccaggtcacc accacatgag caggggtata tcccaccacc cacgcatcca
    34261 atccattggc tgattgaccg accttcacac cggcaggcct gccgatctcc aacacatttg
    34321 gcctgcccaa cgatggccag cgtgaagcct catcactcaa aacatgggtc atcaaatagg
    34381 ccaatgcagg ggtcaacaca ggcttcgcct gcggcagaga ccaatccaac aacagcgaac
    34441 gatccacacc ttccacacgc agcacagcgg aaggcagaaa ttcatcgttc atattctggc
    34501 caaaataaac accctgcttg ccaaacacac catacgcacc cgccaattgc agcatcgtca
    34561 cctgcttatc cagagcaacg ccaaaagacg acgcaatccc gctcacattc tcaaccccca
    34621 tttgcacctt caatgcctcc atcggcactt gataatcgtt cgccagcgca agccgcagcc
    34681 gcaccggtcc gtgatattcg ccatcaaaat tctggatgat gccttcgccc gggatgtccc
    34741 acgccaatgt agcaggactc aacccgcggg tgaatcctgt caaataaaca aacgcatcca
    34801 aactggaccc ggggctgtgt gcggtcacga gcggcgtttc ctgcccttga acagtttccc
    34861 ccaccaccgc caacacctgg ccatgattcg ggtcgaggat cagcgcactt gcagatgaat
    34921 ctgtcagcgt cactgcaggc ggcagtgaag gcagaaatcg cgcggcatca caggcctctt
    34981 tcggctcttc caacccagcc atgcgcactg catacacttc agtgacacaa gccgcttgct
    35041 gttgcaggtc ataatccagc gtggtgatga tgttgattcc gccgcgctcc acacggtcac
    35101 gcggaagttg ggtatccaac tgggcgagga ccatgttcac gaaagccgga gcaggttcag
    35161 agtcgggttg attcgcatca tcctcagtcg aagcgcggat ctcaggctct tctgccaggg
    35221 caatttccgt tgcttcatcg ctgacaatgc cgagtccgtt caacacataa agcacttcac
    35281 gcccgcgttg taaagccact tgcggagcat ccagcggatt gagcgccgga gactcgcttg
    35341 ccgccgccag gatcgcagac tcagccagag tcaactgctc caccgccttg ccaaaataca
    35401 actgtgaagc ggcttcgact ccgtaggcat aattgccaaa gtgcgcgctg ttgagatacc
    35461 actccatcac ctgtgtgcgc ccaaactgtg ccgtgatctg cgccgcaagg atccgctcac
    35521 gaatggcacg ccgcagtgag ggtggttcgt tgtaaatgac cagcccgccg atcagttttt
    35581 gcgcaatggt cgggtgctga ttcgcatcgt ccagcccttg cagggaatac cccgaatgtt
    35641 ttcgaaactg cgggtcttgc actgcgatga tggcatctat taaattggca gggatatgct
    35701 ggggattctg cgggttggcg ggaatataac gccgttgttc gtccacgggt gcaaacgtaa
    35761 aaaggatgtg cgtgcctgtg cggtcgtaga tatgggtggg ttcgagcaaa agcccatcag
    35821 gcgggttaag caaacgcgga aggatttcca cggaaggcaa ttcacgcgtc acatcggcat
    35881 acgcaaacgc acccaaaatg atgaacacac ccaacagcag cgataaaacg atccccccgc
    35941 tgagaatcgc gccacgcgca ttattcgcct gtttgttttg cttttgtagt ctgcgttcac
    36001 gccgcgcacg caggacgggc agatattttt gcatacccct att
//
LOCUS       DAOQJC010000029        35811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_522_length_35811_cov_9.600934, whole genome shotgun sequence.
ACCESSION   DAOQJC010000029 DAOQJC010000000
VERSION     DAOQJC010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 35811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35811
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_522_length_35811_cov_9.600934"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3865)
                     /locus_tag="PLE39_06125"
     CDS             complement(<1..3865)
                     /locus_tag="PLE39_06125"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNE67937.1"
                     /translation="MFEKKEYFYKVALFIGMAVILFLVSGIFQVDAASAALGQITATP
                     TPVVTTLPPNGSNVQNSFGPSGVGPFVSTIYYVNISGGNDGNTCTTIAAPCQHIQEAV
                     NKAVAGDVINVATGTYTFSSNGTPNVVIINKNLKLSGGWNSGFTTQTGASVIDGANAN
                     NGILAVSGTVNVENFVVKNSKSYNGGAVYIVNGSFTLTRSTLMNNVATQHGAGIFLDN
                     GTLNVVNSTVTGNKATSGGGGIYVSNNAAGSAFIQNSTVAYNTAGTGGGIRRSNGTYN
                     ITNSIIANNSSSNSGPDCAGTIAVANYNIIEDVTGCSITGGSNNLNVDPAIENSLSGS
                     ILSHALMLGSPAIDAGTSVGCPSTDQLGLSRPQGSACDIGSIEYMDFPPTVLEVTRAG
                     LNNTSAALVAFAVNFSENVTGVDANDFALTTTGVSGAVISSVSGSGSIYSVTVNTGSG
                     NGTIRLDVLSNGGIVDAGANSLSSAFTSGEVYTIVKTGGGGPSLIAYSDLVVDEGRNR
                     LYGADKNGNKIDVVDLTDLSVIGSYTLAYGTSPISLDLSPDGNELAVAQSGTGYVAFV
                     NLSTSSISQIPSALSGSSTKVTDVIYGRAGILYVLSSNGLHVIDLTLSPHSEIVTQYL
                     YDTEVYEQRFGAISSDKNTLYIVTGTCCSGYDSLGKYNISNGLAKPVLLDRTHLYATG
                     YVKNIRLNLINDENLLVSNFAVYNTSNLTPRARNSLYSLVTPAATLPGKDYYVVLYDN
                     GNLETDTLNFHDVDGSYPLSSASTNVIGTPGAIVANNNGNTLFVSSTGGMAKFNLGVT
                     PPGDAVPLPTSLHHYRDFVFDIPRGVAYGTDESGRVDVVNLSTSSIVKSYLLPSGANP
                     IGIDLSPDGSELAIALNGLEKLLFIDPVTGATIAEVTPDLSDSIYYVNRPFDVIYGRS
                     GRLYSDGDPVGGTDYLHVINTVTHSWVSKSPYPVLLRTGPELEISADNQYVYANEIFS
                     PNNIFVLDVRTDNITKLYSGPHGPVSAPKFTIAPDGSKIFTSSGQVWDGIMQSQLGAL
                     EGAPGNLIEFIPGQNMLVLSDIDTGSDVLKFISAVDYHLISTYTPSSPGGSITEMEVS
                     PDGNMLIMNINNEVRVMSLDPTAPAGAWTVGGDGQATTPGTQFPAPLVLLIENYLGQP
                     IVGRNVTFTAPASGPSGTFQGTNSRIATAVTDANGHAISPMFVANNQQGTYNIIASVN
                     GWEIPFELINVDFAPYVTSVYRISPDPTAAASVDYTVTFSEAVTGVDKDDFTLTTSGV
                     TGASIATVTGSGSSYTVKVNTGSGNG"
     gene            complement(4140..4907)
                     /locus_tag="PLE39_06130"
     CDS             complement(4140..4907)
                     /locus_tag="PLE39_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE67938.1"
                     /translation="MPITHETQKTLINDKAETGDALCMQVRDLHFSYPDGHSALHGVS
                     LKLCNGDKVALVGPNGAGKSTLMLHLNGILSGNGEIEIRGQRLTRDNLPAIRAMVGLV
                     FQNPDDQLFSPTVFEDVAFGPLHMGLPEVEVRARVDAALEAVKMTAYKDRLSHHLSVG
                     EKKRIAIATVLSMNPSLLILDEPSAGLDPRARRTLINLLRELPITMLVSTHDMKLVQE
                     LFPRTIVMDEGRVVADGLTTEILENEELLTAHGLEKP"
     gene            complement(4907..5698)
                     /gene="cbiQ"
                     /locus_tag="PLE39_06135"
     CDS             complement(4907..5698)
                     /gene="cbiQ"
                     /locus_tag="PLE39_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HNE67939.1"
                     /translation="MHADAFDRYHDKESFVHRLDPRVKTIVTVVFIVSNALLPDGAWL
                     AFVLAWVFLLGINLLSNLGFMYTFKRSFVALPFALVAVTVLFSIPGNPVSSFHFLMWD
                     FTITDAGLLRFVSILIRSWLSVQMAIMLVAVTRFPDLIHALEHLHVPAILTTIIAFLY
                     RYLFVLVDEVYRLLRARESRSAAVSGQRSGGSVVWRAKVAGNMAGQLFLRSYERSDRI
                     YNAMLSRGYTGHLYTLNPHEMKSRDYLVTALVVVLIFVMQILGRL"
     gene            complement(5714..6655)
                     /locus_tag="PLE39_06140"
     CDS             complement(5714..6655)
                     /locus_tag="PLE39_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNE67940.1"
                     /translation="MFYSPAPLHIPDGFLSVLVSIICWVITVITLGIAISKTNKSLGE
                     RQVPLMGVMAAFIFAAQMINYPVLGGTSGHLLGGALAAITLGPWAGILVMTAVITVQG
                     LLFQDGGLLAMGANILNMGLLTAGVGYGLYRSVLGKGRGIQLAVAGVAAWLSVMAGAL
                     ATSLQLWLSGTSDLRLVIPAMLSVHALIGLGEALVTVAALGFIFRTRPDLLDEKSASA
                     QGGRGWVVAGVLITLAVLLLSPFASADPDGLERVAINMGFIESGQSAPYEIIPDYAIP
                     FLGETPISTIVAGGVGVLVVVAVAFLLGRSLQQRNAG"
     gene            complement(6660..7085)
                     /locus_tag="PLE39_06145"
     CDS             complement(6660..7085)
                     /locus_tag="PLE39_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNE67941.1"
                     /translation="MTCADLYTPELRARGYRMTPQRMAILHTLHHAGTHLSPTEIYEA
                     AKKDLPGLTEPTVYRTLEFLAENGLARPAHMGNGHLVYEIARHEHHHLVCRICGDEME
                     VEHALLKSLYQKLESTSGYKLTDSHVTFFGLCPSCQKGK"
     assembly_gap    7159..7258
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7408..8115)
                     /locus_tag="PLE39_06150"
     CDS             complement(7408..8115)
                     /locus_tag="PLE39_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67942.1"
                     /translation="MDFKKLFYLLVLITLTACTLPVDEAPPPTALTTATPQPALTLPA
                     TFTPDPTPTYSPTPTLAINEIRTPVQFYDFCADNRGYILIEELIQAITQENGTAFEKL
                     VSPAIGVDIRYYRNDKVVNYTLDTASIFQDTAEVDWGISFGSGQPTVGTFREIVLPSL
                     QKVFTPASLIVCGQLQIGNASYFPEWPYPDMNFYSIYFPGTDEFGGMDWQTWAVGMDT
                     ITGKPYLAALVHYVWEP"
     gene            complement(8185..8595)
                     /locus_tag="PLE39_06155"
     CDS             complement(8185..8595)
                     /locus_tag="PLE39_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67943.1"
                     /translation="MTQKNKKEGLSAGDYGIVIGGNVNNSNIVMGDGNTVSLSATSLD
                     SFFKDMEQMVDEQPNLKPDDKEDIKAELQEIQVALKAKEPDETFLARRFRNLKRMAPD
                     ILEVAIETLKNPIGGVAEVIKRVAKKMAEDAAAS"
     gene            complement(8642..11275)
                     /locus_tag="PLE39_06160"
     CDS             complement(8642..11275)
                     /locus_tag="PLE39_06160"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE67944.1"
                     /translation="MTEKKQPSKKTSKPSQPEQEIQAGDQSIAVGGSVTDSAIIHGET
                     IVLADRFWRDLKPALPPEKLREATAAYLAYLTDRHYYLSLKGMGVSDRVPLKLKLLDL
                     YVPLKARMELPEGETWKRDVSLAGRNVSAEEEEMFNLGEPVPLLKVMQKHSGVIILGD
                     PGAGKTTFVKYLALRLARGEGKKIGLNDYLPILLPLAAFANAITARDVRLDDFIAEYF
                     DGIGADLPVGPMLKEALKAGRALILLDGLDEVRDISLRNTVVERVVDFFAFHRREGNK
                     FVLTSRVVGYRAVRPSAEDLVECTIVDFEEDEIEEFVKYWTTALEKQAQGNTAVARAD
                     AETDRRELLDAIQQNPGVRQLASTPLLLTILALMKRQGVSLPERRVQLYDQYVNTLLS
                     TWNRARSLSGRAPGRDIDELQTVRILAPLALWMHEVSPGVGLVGREDMRRKLEELFLE
                     RGDVSPHQAARQFLIDVREHAALLLERGPGEYGFIHLTFEEYLAAVALALMGQGNPAP
                     IIEVLSAHVGEQAWREVTLLTIGYLGIRQQLPKVAGEVVEALVNNQPGPPGEAVVLAG
                     DAVLDTWPGGVSPQNREYVIQALVDTMQDGAIRPDLRRHASLLLGRLGWRPGDLDRFA
                     EIPEGEYQAGIKKEPKTIPYNYFIGKYPITNIQFARFVKEDGYHTQEYWSYDGWEWRT
                     GKYDSRTLEAVAHDWLEHRPLAKRNIPYYWHNIELSNPIVPVVGVCWFEAEAYCNWLA
                     KKIVAVPEGYTIRLPREGEWERASRGEDGREYPWGEGFDKAAANTWDSDTKGSGLGGT
                     TAVCTFPAGVSPNGVWDTSGNVWEWTSSWYDEDRKYRVVRGGSWIGYQWFARSSFCNW
                     SIPLMFNDDLGFRVVIAPKDL"
     misc_binding    11476..11791
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11836..11988
                     /locus_tag="PLE39_06165"
     CDS             11836..11988
                     /locus_tag="PLE39_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67945.1"
                     /translation="MNPVTEQDEQTTYMQERAIETPTDDSLPAAQVIEEQNKEAEQNY
                     TPRFIP"
     gene            12010..13377
                     /locus_tag="PLE39_06170"
     CDS             12010..13377
                     /locus_tag="PLE39_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNE67946.1"
                     /translation="MENTTRFLLNETDLPKFWYNIAADSPVAPNPVLHPGTLEPVTPD
                     FLSVLFPMDLIMQEISTERYIEIPEEVREVYKLYRPTPLLRARRLEKALDTPAHIYYK
                     YEGVSPSGSHKPNTAIAQAYYNKIAGTKALTTETGAGQWGSSLAFACSVFNMELEIYM
                     VKVSYHQKPYRRNLMEVYGATVYASPTDRTNYGRSVLAENPDNKGSLGIAISEAVEAA
                     ATSNGTKKYSLGSVLNHVLLHQTVIGEEALKQMDMANEYPDVVIGCAGGGSNFAGIAF
                     PFLRENLKNGKRARLVAVEPTATPSLTKGVFTFDYGDTAKMAPIVKMHTLGHDFMPPG
                     IHAGGLRYHGMAPSVSALVDAGHIDAVAVKQRETFEAAIQFANAEGIVPAPESAHAVR
                     AAINEAMDAKAKGEKRVILFNLSGHGNFDMAAYESYLGGKLEDFEYPAEAVQEAMKKL
                     PQVSL"
     gene            complement(13439..14599)
                     /locus_tag="PLE39_06175"
     CDS             complement(13439..14599)
                     /locus_tag="PLE39_06175"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNE67947.1"
                     /translation="MALFASVGSAQALDGREAGLQAAHQALNHLGANSANFAIVVASH
                     QYQAREVLNGVTSLLGELPMVGFSSPAGLTSEGHFQHSVVVALLSGDFQADAMWLPGY
                     AQSGRETAAKVADHAAAREERQSLLVFADGFNGDAEQFCGAMPPGLNILGALSSGDLN
                     TGNTYQMTGTQTGTGGMVAAFLRGEIKTGVGYGHGWDQVGKQFRVTRSRGFWLRTLDG
                     RPASEAYAALFGYPARDWAFPPLSYLARLYPLGVEVGDDLITRSPIRVEADGSFRMNA
                     SIRDGIDAYLLVGSRAACERAARDATQQALLQLGDSKPVFALVLVDVAWQMLLKAHPG
                     VEIEAVRDILGTNVPIAGGYTLGQVVSSENVTPKFLNQHIVVVVFGEPVHNS"
     gene            complement(14609..18670)
                     /locus_tag="PLE39_06180"
     CDS             complement(14609..18670)
                     /locus_tag="PLE39_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE67948.1"
                     /translation="MAARNFQRSAQINLPRNGTLFNQILQWTVFAYQAIAVIIFLVGM
                     YLSVRWFQQPFLGALYEHTMVFNGTGPSEAMPEWALFKQVVVGDQMVAIDGTPVRNTR
                     EVRSILQGHVPGENVTVTVKSESGQSRDVSVTLHNFPASSRTTYFIVPSILGLVFIIA
                     SWWIFGLRRNEPAGRAFSLFTSSLAIVTGAYFNLVTTHEFTVFWTFALAIMGGALFDL
                     GLSFPLEPRVVINRPYLRWVGLIIGIVLAVFNLTTLFNFERPTAYIPVWQYIYGFNAI
                     SIIFYIGLSLYQAVSAQSPVVKTQARAILLGAMLAFVPAGIWLGLNAFQLLWKETGSS
                     ASQPLNFSPYLFLPLAFFPLTIGYTILRFRFLRTDDFVRRGLMYFFLTAFVMGGYGLV
                     VAGVGLLVNSSSSDALVSNPYLVGGFMLLLALLLEPFRVRLQSVVDNTFFRGERALAE
                     KLEDFSHKLATALDLNSISTILREQIASTLTPSRVHIYTYDSLNDFYSALPGEDRRPT
                     SDIRFTTTSPFVRYFEKEHLPLYLDTTAALPSDLQSEQSRLALLGARLFVVLPGKNRI
                     NGWLALGPRLSGQPYNPRDLRFLENICDQASVAIERMQTVARLERQIQEMNALTRVSQ
                     GVNVTLTFDDVLELIYAQTAQIIPTSHFHITLYNEASDYYYYGFCVENNERISERENL
                     PFPVTAGLSPEVIAKSRQIMTQDYIHECQARSLTPIVENVFAWMGVPLNAGAKSIGAL
                     SIGSRDGATAYTRGQLELLQAIADQTAGAIVKARLLEETQQRAHQLSTLNEINRQLTS
                     TLELSPLLQNILENAVGILNCEAGSLFLVDEQTKELVFKVTVGPVAANLIGQRLPPGT
                     GIVGRAVQSRGPVIENEENRSASRFRGVDQQTGFVSRSLMAVPLQVKDRVIGVIEVIN
                     RRDGLPFVTGDQDLLTAFAGQAAVAIENARLYTLTDQELAARVEELSVMQRIDRELNA
                     SLEMDRAMHITLEWALRQSDAEAGLIGMLEEGKLRVMAHQGLDDRMANLPEDQAMKIE
                     LPAMIQSIENGQPQSLSVATSNDKLLSSAHTQMIIPIRRETTVIGLLHLESISDSQVD
                     IGFLTRLTDHAAIAISNAQLFGEVQRANLEKSRFVSFVAHELKNPMTSIKGYTDLMAA
                     GAVGPISDMQANFLNTIKTNVIRMDTVVSDLNDISKIEAGRLSLAYKVTEVPPLIDDT
                     VRSLKRQIEEKKQTLEIVLPEKLPVIWADPNRITQVLVNLLSNSYKYTPESGILQIGA
                     EEASNLGETNSSMRVVHIWVKDNGLGMTPEDQKKVFQQYFRTEYSKEVASGTGLGLNI
                     TKNLIEMQGGSIWFESEFHVGTTFHITIPVAEE"
     gene            complement(18838..20769)
                     /gene="gyrB"
                     /locus_tag="PLE39_06185"
     CDS             complement(18838..20769)
                     /gene="gyrB"
                     /locus_tag="PLE39_06185"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNE67949.1"
                     /translation="MAEKEKKINYDVSSIQALEGIEHVRKRPGMYVGGTDIKALHHLV
                     YEVVDNSIDEALAGVCNRINITIHPDSSVTIEDNGRGIPVGPHPSKKDANGKPMETLD
                     VVMTVIGAGGKFGGGGYKVSGGLHGVGVSAVNALSEWMVTEIKRDGKLWRQEYKRGVP
                     QGSIKQIGKVKDETGTKQTFRFDKQIFTEDIDFRFDTLVQRFREMSFVARGITIKFVD
                     ERADREMTFYFEGGLTSFVRYLNRNRENLHPVVHVEKEIENIGIEAAIQYTDAYTESV
                     YSFANTINTVDGGTHLTGLRSSLTRVINDYARKNGLLKDADPNFSGDDTREGLTAIVS
                     VKHPDPQFESQTKVKLMNPEAQTYVTQVVGETFGTFLEENPQAAKAIIAKCLTSARAR
                     DAARKARDLVIRKSALESLTLPGKLADCSERDSSKTELYIVEGDSAGGSAKQGRDRHF
                     QAILPLRGKILNTERARLDKILGNNEVRSLISALGTGVGDNFDLEGLRYGRVIIMTDA
                     DVDGSHIRTLLLTFFFRYMPLLIDEGHLYIAQPPLYRVAYKNQVKYAYSDKEKDKLVK
                     EIGSDKVGIQRYKGLGEMNPEQLWETTMNPTNRTLLLVTVDDAAEADRTFDMLMGDAV
                     DPRKKFIQTHAKSVRNLDI"
     gene            complement(20814..21803)
                     /locus_tag="PLE39_06190"
     CDS             complement(20814..21803)
                     /locus_tag="PLE39_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67950.1"
                     /translation="MEVSRTENLPTPPGIINSIKAGFDMIASHITAILLPLLLNLFLW
                     LGPRLRMDALFNSIKDDVVSIWRTGGVPAEDIQRILTWYETTIPNINLFWIVRTLPIG
                     ISSLLFPQQPAATPLGNPLVLQVDAVNLMGWVFLLNLLGWVGGGLYFRSVAWLTVADK
                     ENQPIQISRAIVQTVLTSIIWSILSVIILVPAFLVLAVVLQFSAFFANLLILFMSLIS
                     MWVIVPFFFWPHGIFLRRQNFLTSILSSVQMTRFTLPTSSMFVLTVFLLSVGLNFLWS
                     IPPEDSWMTLLGIFGHSFVTTALLASSFVYYRDMTAWLQAVIEKLRPNAVTKA"
     gene            21866..22681
                     /locus_tag="PLE39_06195"
     CDS             21866..22681
                     /locus_tag="PLE39_06195"
                     /inference="COORDINATES: protein motif:HMM:NF040134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67951.1"
                     /translation="MQERHSYLPSADRVGVLIASVLLTFALTRLIQSPQFTLTIELPG
                     FYFAYPLTLGTAMTILAAALAAAGMDWLTRGHPNLGEKPNIEHLMLPTLTTFVIGSPL
                     ALLPESIAWWVALAFAASLLVIVFLAEYITIDPSAPSYAFARAGLTALAYALFLILAM
                     SLRFSGVRMFLLFPVVLVVAGLISLRILHLDGTDRWDFPWALGIGIVCAQIGAGLHYW
                     PLTPIQFGLALTGPLYALTMLSASLTENTPLRRAAVGPILILGLSWVSAFLVK"
     gene            22698..23096
                     /locus_tag="PLE39_06200"
     CDS             22698..23096
                     /locus_tag="PLE39_06200"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNE67952.1"
                     /translation="MNSLTLTNEQLQKMIDHVDSHAPLEACGLLAGKGSTVEAVLVVR
                     NQAQSPVRYVMDPIEQLHAFEWIESNGLDLIGIFHSHPTGPETVSPTDIAEAAYAVTY
                     VILARVNDAWRTRGFWVEHGGFREVDLQIL"
     gene            23152..23844
                     /locus_tag="PLE39_06205"
     CDS             23152..23844
                     /locus_tag="PLE39_06205"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNE67953.1"
                     /translation="MQIVFDIALILLAYVFGSIPFGLLIVKLKTGKDIREVESGRTGG
                     TNAMRAAGFWAGFATAMLDILKGAVGVWVARWLSPDQTWVHVLAPIAAILGHNYSVFL
                     IERDENGKIIRFRGGAGGAPSVGGAMGLWPGSILIILPLGMLTFFSIGIASITTMAVA
                     LFAIIVFAIRASQGLLPWAYVWYGVGAELLLIWALRPNLKKLFEGNERVVKYSLNGWL
                     RARREAKTAAGK"
     gene            23976..24512
                     /locus_tag="PLE39_06210"
     CDS             23976..24512
                     /locus_tag="PLE39_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE67954.1"
                     /translation="MKSIDQNDISLRRWSKNDLPLLEGLLGDPAMTKHIGGPETPEKI
                     RARLERYCVDSSISKIHMFVIVLQAKQLGIGSIGFWERQWKNQTVWESGWRILPAYQG
                     RGIASKAIALVMERARAEQKHRYVHAFPSVENDSSNALCKKTGFVLLEEVDFEYPPGH
                     TMRSNDWQLDLFSHMSED"
     gene            complement(24600..25379)
                     /locus_tag="PLE39_06215"
     CDS             complement(24600..25379)
                     /locus_tag="PLE39_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNE67955.1"
                     /translation="MKRIAFIILIAVLSSCNSTPPASLPTESPTPPVVTGEQIIQFSG
                     YDWIVRDGGLSGPGPNTWNGNNVWVDENGDLHLKITHTESGWTCAEVTTTERLGFGIY
                     QFQVIGPIDQLDANVVFGMFNYPTEDVGPNATNEIDIEYAHWGNPEWPIGNFTVWPAQ
                     SGVEQTSESFPVALNGSYTTHRFVWESQKILFQALHGHRDDNENEIVNWLYKPDEPLV
                     HISQQAMPVHINLWLFQGQPPVDGNEVEVVIHSFTFASLGK"
     gene            complement(25376..25675)
                     /locus_tag="PLE39_06220"
     CDS             complement(25376..25675)
                     /locus_tag="PLE39_06220"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNE67956.1"
                     /translation="MYAMCGRLIAKPNQRGQLVEILLRAAQMVGQLSSCHLYAVTEDL
                     ADEVTVWVMEIWDDKEAHDASLKDEHVRALIAEAMPLMGGAPSGSELKVAGGHGV"
     gene            25955..27121
                     /locus_tag="PLE39_06225"
     CDS             25955..27121
                     /locus_tag="PLE39_06225"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNE67957.1"
                     /translation="MRKSVYLVVTVAILFALLTTVTGAAQAAPAAQAKWTVMVYISGD
                     NNLEDYVVKDLELELAPTGSSANVQVVALADRGPGYDISRGDWQTTKLFHVTTGMIAD
                     SASAVADWGERNMGDKQTLIDFVSWSKTNYPADHYALYFWGHGWNWHPGYVMEDVTNA
                     DTLDLDELKAALPSLGFIDVVGYDGCNMASIEVFNAWHGYATAVSSSQEYVGWDGIEY
                     DVVLAQLAANPNMTADQVAIATSQSASNDKTWSAVAVDSRFNALYTAVDQWSVALKNG
                     LAANRKKYDRAFGATQDFWQAPMDKDLYDMAYEIKRNVADTNIKNKSQAVMDAIAGVV
                     LNEHHVNGYPDAHGITIYYVSSAAGKDSNYTYYRSTVDFALTTGWDEFLDAYAK"
     gene            complement(27614..27997)
                     /locus_tag="PLE39_06230"
     CDS             complement(27614..27997)
                     /locus_tag="PLE39_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNE67958.1"
                     /translation="MCPVRMSKVESAVRVVIEFNEAFNRHDVEGMMRLMGDDCVFENT
                     SPAPDGTVYSGREAVTQFWQDFFRESPQAHIEIEEIFGYGIRCVMRWKYSWVDVTGNK
                     GHVRGVDIFQLKDGLISQKLSYVKG"
     gene            complement(28031..28900)
                     /locus_tag="PLE39_06235"
     CDS             complement(28031..28900)
                     /locus_tag="PLE39_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE67959.1"
                     /translation="MNTIILSILLGIGGMFGWGIYDFLGGVFAKQIGSFRSLFWSQLA
                     GLISVLILAVVARLTVDIPILVVALSLIAAILYSAGYLFFFKGFETGNVSIIAATMNL
                     WAVFTMLFAFVFMGQRLSALKTLGVLMIICGATVASLNWNEIGNRKFQLSAGVREAIL
                     GAFFFGVFWNVSEIISEKVGWLLTTLLIKAGIIIFLSIFSFLTKQGIGLTHVSANTKA
                     IIFLMGVIEAGAVALVNYGLTIGDAILITPIASALSIVTITLAIIFLKDKINKIQGLG
                     IVAAIVGIIVTAF"
     gene            complement(28984..29670)
                     /locus_tag="PLE39_06240"
     CDS             complement(28984..29670)
                     /locus_tag="PLE39_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67960.1"
                     /translation="MNLTVDQLAIIDKQLLDGNLVAAVRELRQFTGVQLAEAKIFVDD
                     RLAYLRMTRPSQFQTAQKASPLPQPWVLQQIKPLPDEALREFRALLSHIDATLCGLNY
                     VTTLSVHAATVPASDLPIPELMMTFFPKSTPFEAKIRSAQPDELVEYVTECFRYVGGG
                     SAGPQLIDALLSEYWRHLNLLVPLETSTLYYYGSDVGLPGHYIFWRFAYLLHDSKTNR
                     CVVITGNSTD"
     gene            complement(29762..30775)
                     /locus_tag="PLE39_06245"
     CDS             complement(29762..30775)
                     /locus_tag="PLE39_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE67961.1"
                     /translation="MSDKQVLVTGASGEIGQALVQGLAARGGYRIVTSDFMPLPDSIK
                     HLSAEHVQGDLVYKIKTFYDYDFDIIFHLAASLSSKAEVATEEAHRINVEGTMQLLML
                     AAYRSEKYGKAVKFLFPSSIAAYGMPTLDAKRKAGLVKEDEWDYPHTMYGCNKLYCEK
                     LGMYYGKFYGQKHLDEKPPVMLDFRAIRFPGLISAFTLPSGGTSDYGPEMLHAAAQGK
                     PYSCFVRADTKISFMAMPDAIKSLLMLMDVPREKLSNTVYNIAAFALTAEEFRQRALK
                     AFPDAKINFEPNPRRQGIVDSWPEDVDDSRARKDWNWTPDYDVDKFFDEYFLPEIRKR
                     YGK"
     gene            30853..31662
                     /locus_tag="PLE39_06250"
     CDS             30853..31662
                     /locus_tag="PLE39_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67962.1"
                     /translation="MMFLRFQIPAKKFLLTALTTLMLTSSCAVVDAMLTTPTPQIPTE
                     TPIPTATIVWFPPSATPSAQPFSTQLPTPEMRPGLGSIVLTDNFSNEEDWNTATSGQA
                     SAAIENNQLILASQSKVYMLSLRRDLALDDYYAEITARINLCRGDDSYGILVRANAVA
                     YYRFALTCNSTVSAERVSVGTRQLLQSPLPSGDAPPGAPGEVRIGIWAVGTEMRLFLN
                     GRYQFSISDTNYPSGTIGVFVNSAGDTGTVISFTELTVRQVNAALPAESSP"
     assembly_gap    31735..31834
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<31835..32928)
                     /locus_tag="PLE39_06255"
     CDS             complement(<31835..32928)
                     /locus_tag="PLE39_06255"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE67963.1"
                     /translation="MDWLTGSKQGEARKLISQLADSTKRDAASQSLLKLGADAVPALM
                     EALQTQDLNLLLLYQHLLARISPAQPQLIKVLSTAHPVIRGRIIEVFGFSKDKALIPV
                     LLDALKSEYFTVRAKALWALAAIGEERTVSDLLPLLKDPEDEVRIAACKAVAKFRDPS
                     TFDEITNVLLDDLKIEVRQAAAKALGDTRHPAAIPFLMEALRDSYWWYEKEQAVSVLL
                     TAIENMGEAVVYPLIDALADKEGTVRKFAAMILSKLGDIRAIEELGMALYDLHHEVGE
                     AAAEALTKFGSQAVDILIESLNHPEAAIREHAVTALGKIQDVRVAPVLIEMLHDPDRV
                     VQKKAMASLANLRDERAIPALRQIAENRADR"
     gene            33150..34109
                     /gene="pdhA"
                     /locus_tag="PLE39_06260"
     CDS             33150..34109
                     /gene="pdhA"
                     /locus_tag="PLE39_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNE67964.1"
                     /translation="MKKEQQLDLYYQMVLIRRAEERGAELYQQGKIGGFMHLYIGQEA
                     VSTGLIAAREPRDRVITAYRDHGVAINCGISTNEVMAELLGKATGMSKGKGGSMHMAS
                     TEKNMWGGHAIVGGHLPVAAGLALGDQYAKNDNITICMFGDGATNIGYFHEALNLAKT
                     WGLRVLWVCENNQYGMGTAVERASAVTEIRQKAEGYGMKNGQVDGMDVMKVYEAAKEA
                     LDFIRKEGQPYLLEIDTYRFRGHSMGDPERYRKAEEVKQWQENDPIGIYNKYLLDKKI
                     ATRKQLDEIESRADEEVNKAVEFAEASPEPTMEDLYADIYADN"
     gene            34201..35175
                     /locus_tag="PLE39_06265"
     CDS             34201..35175
                     /locus_tag="PLE39_06265"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012009660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNE67965.1"
                     /translation="MAKITMREAISQALMEEMDRDPAVFIMGEEVGVWGGTYAVTKGF
                     FDKYGADRIKDTPIAENAILGAAIGAAMVGQRPIAELMTINFAFSAMDYIVNQAAKLR
                     YMFGGQFMLPLVIRAVGGGGRQLGATHSQTPDAIFAHFPGLKVVSPGTPEDAKGLLKS
                     AIRSNDPILFIEHATMYQVRGEVPEGEYTIPIGKSKIQRPGKDLTIVTYCKGLELSMK
                     AADELSKQGIEAEIVDLRTLRPLDMEPVLESFKKTNRAIVVEEGWKSYGVGSEIVSRI
                     YEEAFDYVDAPIIRVAQKEVPLPYNRTLEQAALPQVHDIVAAAKEVLK"
     gene            35175..>35811
                     /locus_tag="PLE39_06270"
     CDS             35175..>35811
                     /locus_tag="PLE39_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HNE67966.1"
                     /translation="MAETINMPKLGFDMAEGLLVRWVKQVGENINKGDVLAEIETDKA
                     TVEVESSASGVVLSHIVDQGSMVPVNAPIAVVGAAGEKVDAPAAAPKAEAKAEEKPAA
                     QTQPTAASAPSGQASAPADNGPVKASPLARKVAKDNGVNLASVQGTGPGGRVVRKDVE
                     AALAGGQTSKVESRPSAPITNYQLPVSTEDKVTPTTKLRQAIGRRLVESKQT"
BASE COUNT         9398 a         9438 c         8420 g         8355 t
ORIGIN      
        1 ttccattccc gctgcctgtg ttgaccttaa cggtatagct actccccgaa ccagtgactg
       61 tggcaatgct cgcccccgtc acaccgctgg tagtgagcgt aaagtcatcc ttgtccacac
      121 cagtaacagc ctcagagaag gtgacggtgt aatccacact ggccgccgct gttgggtctg
      181 gcgaaatgcg gtagacacta gttacatatg gagcaaaatc aacatttata agctcaaaag
      241 gaatttccca gccatttacc gacgcaatga tattgtaagt gccttgttgg ttattcgcaa
      301 cgaacattgg tgaaatagca tgtccattgg catctgtgac ggcagtggca attcttgaat
      361 ttgttccttg aaaagtacca ctgggaccac ttgcgggtgc agtaaaggtt acattcctgc
      421 caacaattgg ctggcctaaa tagttttcaa tcagtaacac aagaggcgct ggaaattggg
      481 tcccgggtgt agtcgcttgt ccatcgccac caactgtcca tgctccagca ggagcagtag
      541 gatccaggct cataacacga acttcattgt tgatattcat aatcaacata ttgccatcag
      601 gtgaaacctc catttcggtg atactccctc caggagatga tggcgtataa gtggatatca
      661 agtgataatc aacagcactg ataaatttca atacatcact tccagtatct atatcactaa
      721 gaacaagcat gttttgaccg ggaatgaatt cgattaaatt cccgggcgcc ccttcaagtg
      781 cgcccaactg agactgcatt atgccatccc agacctgtcc actggatgta aatattttgc
      841 ttccatccgg agctattgtg aatttgggtg ctgacacagg tccatgtggc cctgaataaa
      901 gtttagtaat attatccgtg cggacatcta acacgaaaat attgttaggc gaaaaaattt
      961 cattggcata aacatattga ttatccgccg aaatttccag ttctggccct gtccgcaaaa
     1021 ggaccggata aggtgatttt gaaacccagg aatgtgtgac tgtattgatg acatgcaagt
     1081 aatccgtacc gccaacgggg tcgccatctg aatataaacg gccgctacgt ccataaatca
     1141 catcaaatgg gcggtttaca tagtagatac tgtccgagag gtctggagta acctcggcaa
     1201 ttgttgcacc tgttaccggg tcaataaaga gcaatttctc aagcccgttc agcgcaatag
     1261 ccaattcact tccatcaggg cttaagtcaa ttccgattgg gtttgcacct gagggaagca
     1321 aataactttt aacaatactg gatgtgctta ggttgacaac atccaccctg ccagattcat
     1381 cagttccata tgccaccccg cgcggtatgt caaaaacaaa atccctgtaa tgatgcaggc
     1441 ttgtcggaag tgggacagca tcaccaggag gagtaacccc caaattgaat ttagccatcc
     1501 cacctgttga gctgacaaac aatgtattcc cattattgtt agcgacaata gctcctggag
     1561 ttccaataac attagtactt gctgaagaaa gcgggtaaga gccatccaca tcatggaaat
     1621 ttaacgtgtc tgtttccaga ttaccattgt catacaaaac cacataatag tcttttccag
     1681 gcagggttgc tgccggtgta accagtgaat acaaagaatt tctagccctt ggcgtcaggt
     1741 tggatgtgtt gtagacagca aaatttgaaa ccaacaagtt ttcatcatta atcaggttta
     1801 atctgatgtt ttttacataa cctgtagcat acaaatgtgt tctatcaagc agaacaggct
     1861 tcgccaagcc gttggaaata ttgtatttac caaggctgtc atatccgctg caacatgttc
     1921 cagtaacaat atatagggtg tttttatctg aagaaatggc accaaatctt tgctcataca
     1981 cttcggtatc atacaggtat tgagtaacaa tttcggaatg tggactgaga gttaaatcaa
     2041 taacatgcaa gccattactt gaaagtacat aaagaatccc ggcacgacca taaataacat
     2101 cagtaacttt cgtgcttgat ccactcaaag cagatggaat ttgcgaaatg gaacttgtag
     2161 acagatttac aaaggccaca taccctgttc cagattgtgc tacggcaagt tcatttccat
     2221 cagggcttag gtccaaacta atgggtgaag tcccgtaggc aagcgtatac gaaccaataa
     2281 cagaaagatc tgtcaggtca acaacatcaa tcttgttgcc atttttatcc gccccatata
     2341 acctatttct tccctcatcc actaccaggt cagaatatgc aataagggaa ggtccaccac
     2401 caccagtctt tactattgta tatacttctc cactcgtaaa agccgaagaa aggctattgg
     2461 cacccgcgtc tacaattcct ccattactca aaacatccag acggatcgtg ccatttccac
     2521 tacctgtatt gaccgtaaca ctataaatgc taccagagcc gctcacactg cttataaccg
     2581 cacccgaaac tccagttgtt gtcaatgcaa aatcgtttgc atccacacct gttacatttt
     2641 cagaaaagtt aacagcgaaa gcaacaagcg ccgctgacgt attattcaaa ccagcacgtg
     2701 tgacctccaa aactgttgga ggaaaatcca tatactcaat cgaaccaata tcacaagcac
     2761 tcccttgtgg acgagataat cccaattggt ctgtcgaagg acatccaact gaagttccag
     2821 catcaatagc cgggctaccc agcattaatg catgagacaa aatgctcccg ctcaggctgt
     2881 tttctattgc cgggtcaacg tttaagttgt tactaccacc agtaatcgaa cagcctgtga
     2941 catcttcaat aatattgtaa ttcgccactg caatggtacc ggcacaatct ggtccggaat
     3001 tcgaacttga gttattggca atgatcgaat tggtgatatt gtatgttcca ttacttcgac
     3061 gaataccgcc acctgtacca gccgtattat aagcaacggt tgaattctga ataaaagcag
     3121 aaccggcggc attgttcgat acataaattc caccaccacc gctggtcgct ttattcccag
     3181 taactgtgct gttgactaca ttaagcgtcc cattatccag gaatattccc gccccatgct
     3241 gggttgcaac gttgttcatt aaggtacttc ttgtcagtgt aaaactgcca ttgacaatgt
     3301 aaacagcccc gccattgtac gatttggaat ttttgactac aaagttttca acattcacag
     3361 tcccagaaac tgccaaaatt ccgttattag cattggcgcc atcaataaca gatgcaccag
     3421 tttgggtggt aaatcctgag ttccatcccc cggaaagctt caggttttta ttgataatta
     3481 caacattggg tgtgccattc gaggaaaacg tatacgtccc tgttgccaca ttaatgacat
     3541 ccccggctac agctttatta accgcctctt gaatatgctg acatggagct gcaattgttg
     3601 tgcatgtatt accgtcgtta ccgccagata tatttacata atatatggta ctaacaaacg
     3661 gacctacacc agatgggcca aatgaatttt gaacattact tccattaggc ggcaaagttg
     3721 ttaccacagg tgttggagtg gcggtaattt gacccaatgc agcacttgct gcatcaacct
     3781 gaaaaattcc cgaaaccaaa aacaaaatga ccgccatccc tataaacaag gcgaccttgt
     3841 aaaaatattc ttttttttca aacatgaaaa cctccttttt attttccagc ccattaagaa
     3901 gtggtattaa accatttttc accgttttat gtacattacc accttacagg ttttaaaacc
     3961 aatttaaccg ctaatctatc agattccctt caaaattgcc agtgatttta gtcatcatat
     4021 taagggggaa cacccaaaaa tcttgcagaa tcaaaaggac tccagagttt tgtaagcctg
     4081 gagtcctttc tgtttttatt actctacaca tttgtcagcg atttttttcg tctattctcc
     4141 tatggtttct ccaaaccatg ggccgtcaat agttcttcat tttccagaat ctctgttgtc
     4201 aagccatcag cgacaaccct gccttcgtcc atcacgatcg tgcgcggaaa caattcctgc
     4261 acaagtttca tatcatgtgt ggagaccagc atggtaatcg gcagttctcg caacaggttg
     4321 atcaaagtcc gtcgtgcgcg cggatccagc cccgccgatg gttcgtccaa aatcaacaaa
     4381 cttggattca tggaaagtac tgtggcaatt gcaatccgct tcttctcacc cacactcaaa
     4441 tgatgcgaga gtctgtcttt gtaggcagtc atcttcactg cttcaagcgc ggcgtccaca
     4501 cgagcacgga cttccacttc aggcaagccc atatgtagtg gaccaaacgc cacatcttcg
     4561 aacaccgtcg gcgaaaaaag ttggtcatcc gggttttgga acacgagtcc caccattgca
     4621 cggatggcag gcaaattgtc acgagtgagg cgttgaccgc ggatctcgat ttcgccattc
     4681 ccgctcagga ttccgttcaa atgcaacatc aaagtggatt tccctgcacc gttcggccca
     4741 accagcgcca ccttatctcc attgcacaat ttcaaagaaa caccatgcaa ggcagaatga
     4801 ccatcaggat aggagaaatg caaatcacga acctgcatac aaagtgcatc gcctgtctcg
     4861 gctttgtcat tgattaaagt tttttgagtt tcatgtgtaa ttggcattag agtcttccaa
     4921 gaatttgcat cacaaaaata agcacgacaa ccaaagcagt gacaagataa tctctagact
     4981 tcatctcgtg cggattcaaa gtatacagat gcccggtata accacgggaa agcatcgcat
     5041 tatagatacg gtcactgcgc tcgtagctcc gcaaaaagag ttgacctgcc atattacccg
     5101 cgaccttcgc ccgccaaaca acgcttccac ccgacctctg gcctgatact gccgccgaac
     5161 gagattccct tgcccgcagc agacggtaca cctcatccac caacacaaac aagtagcgat
     5221 acaaaaacgc gatgatcgtg gtgaggatgg cgggcacatg caaatgctcc aacgcgtgga
     5281 tcaaatccgg gaagcgtgtg acagccacca acatgatcgc catctgcacc gaaagccagg
     5341 agcggataag aatacttaca aaccgcaaca ggcccgcatc tgtgatcgtg aaatcccaca
     5401 tcaaaaagtg aaaagatgaa acaggatttc caggaattga aaataaaacg gtaaccgcca
     5461 ccaatgcaaa gggcaatgca acaaatgacc gcttgaacgt atacatgaag cccaggtttg
     5521 agagtaagtt gattccaagc agaaaaaccc aggcaagtac aaaagccagc cacgccccat
     5581 ctggcagtaa ggcattggag acaataaata caacggtcac gatcgtttta actcgagggt
     5641 caagacggtg aacaaagctt tccttgtcat gataacggtc aaaggcgtct gcgtgcatat
     5701 gtccctgttt ctcctaacca gcattccgct gttgaaggga gcgcccaagc aaaaaggcga
     5761 cagccacaac aaccagcacc cccacccctc cagcaacaat ggtggatatt ggagtttctc
     5821 caagaaatgg aattgcataa tcggggatga tctcataggg tgcagactga ccactttcaa
     5881 tgaagcccat attgatggct acacgctcta atccgtcggg gtccgccgag gcaaatggag
     5941 aaagaagtaa aactgccaga gtaatcaaaa ccccagccac cacccaacca cgcccaccct
     6001 gagccgaagc ggatttttca tccagcaaat cgggtcgggt gcggaagatg aatcccaaag
     6061 cagcaacggt tacaagggcc tcgccaagac cgatcaaagc atgaacactg agcatggctg
     6121 gaataactag ccgcaaatct gaagtaccgc tcaaccataa ttgaagtgaa gtggccaaag
     6181 caccagccat tacagaaagc cacgcagcca caccagcaac agcaagctga atgccacgcc
     6241 ccttacccaa cacactacgg tacaggccgt atcccacgcc tgccgtaagc agacccatat
     6301 ttaaaatatt tgcccccatg gcaagcagtc ccccatcttg aaatagaaga ccctgcacgg
     6361 taataactgc tgtcatcacc aagatcccag cccaggggcc gagggtaatg gctgccaatg
     6421 caccgccaag taagtgtccg gaggtaccgc ccaatacggg atagttaatc atctgagccg
     6481 caaaaatgaa ggcagccatc acacccatca atggaacctg tctttcgcca agagatttat
     6541 tcgtttttga aattgcaatt ccaagggtga tcacagtaat cacccagcaa atgatggaaa
     6601 ccaaaacact aagaaagcca tcgggaatat gcaaaggtgc aggagagtaa aacatattat
     6661 tattttcctt tctgacagct gggacaaagg ccaaaaaaag tgacatggct atcggtcagc
     6721 ttatagccgc tggtggattc aagtttttgg taaaggctct tcaacaaggc atgttcaacc
     6781 tccatttcat ctccacagat acgacaaacc aaatgatgat gttcatgtcg ggcgatctca
     6841 taaaccagat gaccgttgcc catatgggct gggcgcgcca gtccgttttc agcaagaaac
     6901 tcaagcgtgc gataaacagt gggttcggtg agaccaggca ggtctttttt cgcagcttca
     6961 taaatttcgg ttggggaaag atgagtacct gcatgatgaa gcgtatgcag aatcgccatt
     7021 ctttgcgggg tcatgcggta tccacgggca cggagttcgg gggtatatag atcggcacag
     7081 gtcataggtg tccttattgc aactttttgc aataatgtat agaggagtac aagaaaggaa
     7141 ttcttaccac cctaaaaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa
     7261 aatccgcaca acacctgcac tgtgccacat tgcgttcgcg gaaaaaattc ttgaggcaga
     7321 agtagttgaa cctcaagaaa cttgacctac actgcaggta acgggaaaac tacctccagc
     7381 cttctaaaaa tacttttacc agagccgtta cggttcccaa acatagtgaa ctagtgcggc
     7441 cagatatggc ttgcctgtga tggtatccat gccaacggcc caggtttgcc aatccattcc
     7501 gccaaattca tcggtaccgg ggaagtagat cgaataaaaa ttcatatccg ggtagggcca
     7561 ctcgggaaag taggacgcat tcccgatctg taattgaccg cacacaatca gggaagcagg
     7621 tgtgaacacc ttttgcagag acggcaaaac gatctctcga aacgtgccaa ctgtcggttg
     7681 gccgctgccg aatgaaatgc cccaatcaac ttcagcggta tcttgaaaaa tggatgccgt
     7741 atccagtgtg taattgacaa ccttgtcatt gcgatagtag cggatatcca caccaattgc
     7801 gggggagaca agcttttcaa aagcggttcc attttcctgg gtaatggctt gtatcaattc
     7861 ttcgatcagg atgtagccgc gattatcggc acagaaatca taaaattgga caggggtgcg
     7921 gatttcgttg attgccagtg taggggtggg agaatatgtc ggtgtagggt cgggggtgaa
     7981 tgttgcgggg agggtcaggg ctggttgagg tgtggcagtt gtcagcgcgg tgggaggcgg
     8041 ggcttcatcc acaggaagag tgcaggcggt aagggtgatg agcacaagca agtaaaaaag
     8101 tttcttgaag tccattctgt ttcctttttc gatggtttag ctcaagacga aaaactggcg
     8161 gaggtccgcc agttggttgt atttttatga agccgctgcg tcctcggcca ttttttttgc
     8221 aaccctcttg atcacttctg ccacaccgcc gatggggttt ttgagggtct caatcgccac
     8281 ttcaaggata tcgggggcca tgcgtttgag gttgcggaaa cggcgggcca gaaaagtttc
     8341 atcgggttct tttgctttga gcgcaacttg aatttcctgc aattccgcct tgatatcttc
     8401 cttatcatct ggtttcaagt tgggctgctc atccaccatt tgttccatgt ccttaaagaa
     8461 agaatccaga ctggttgcag aaagcgaaac cgtattgcca tcgcccatga caatattgct
     8521 attattgaca ttgccgccaa tgacaattcc ataatcacca gcgctcaggc cttctttttt
     8581 atttttttga gtcatgctgc ctccatcatc atcaataaac actttgtgga cacagggtct
     8641 attataaatc cttcggagca atgacaacac ggaagccgag atcatcattg aacatcaacg
     8701 ggatggacca gttacagaaa gaggaacgtg caaaccattg atatccgatc catgagccgc
     8761 cacgcacgac tcggtatttt ctatcctcat cataccagga ggaggtccac tcccagacat
     8821 tgccgcttgt atcccacaca ccattcgggc ttacacctgc agggaaagta caaactgcgg
     8881 tcgtaccgcc aaggcctgaa ccttttgtat cgctatccca ggtattggcc gccgctttat
     8941 cgaaaccttc tccccacgga tattcgcggc catcttcacc gcgtgaagcc cgctcccatt
     9001 caccttctcg cggcaagcgg atggtgtacc cctcaggcac agcaacgatc ttctttgcca
     9061 gccagttgca ataggcttcc gcttcgaacc agcacacccc caccacaggg acaatgggat
     9121 tgcttaactc aatgttgtgc cagtagtatg ggatattacg ctttgcaagc ggacgatgtt
     9181 ccaaccaatc atgcgccacg gcttcgagcg tacgagaatc atatttaccg gtgcgccact
     9241 cccagccatc gtagctccaa tattcctgtg tatgatagcc gtcttcttta acaaagcgtg
     9301 caaattgaat attggtgatg ggatactttc caatgaagta attgtacggg atcgtcttcg
     9361 gctctttctt aatccctgcc tgatactcac cttcaggaat ttccgcaaac cgatccaggt
     9421 caccgggccg ccagcccagc ctgccgagca aaagactcgc atgtcttctc aggtcaggcc
     9481 gaatggcgcc atcctgcatg gtatccacca aagcctgaat gacgtattcc ctgttttgtg
     9541 gggagacccc gcctggccag gtatccaata cggcatcgcc tgccagcacc accgcttcac
     9601 cgggaggccc gggttggttg ttgaccagtg cttcaacgac ctcacctgcc accttcggca
     9661 attgctgacg aatgccaagg tacccgatgg tcaacagagt tacttcgcgc caagcctgct
     9721 cgcccacgtg cgctgacaaa acttcgatga tcggagcagg gttgccttgc cccatcaatg
     9781 ccagtgcgac ggcggccaga tattcttcaa aggtcaggtg gataaaacca tattcaccgg
     9841 ggccacgctc aagcaaaagc gcggcatgtt cgcgcacgtc aataaggaat tgacgcgccg
     9901 cctgatgagg tgaaacgtcg ccgcgctcta aaaacagttc ttccaatttg cggcgcatgt
     9961 cttcacgccc caccaaccca acaccagggc tgacctcatg catccacaaa gccaacgggg
    10021 ccaaaatacg gacggtctgc aattcatcaa tgtcacgccc gggagcacgt ccgcttaaac
    10081 tacgggcacg gttccaggtt gaaagcaggg tgttgacata ttggtcgtac agttgaacac
    10141 ggcgctcagg cagggaaacg ccctgccgct tcatcaatgc cagaatggta aggagcaagg
    10201 gtgtggaagc cagctgccta acgccgggat tctgctgaat ggcatcgagc agttcacggc
    10261 ggtcggtttc cgcatctgca cgggcgaccg cggtatttcc ctgagcttgt ttttcgaggg
    10321 cggtcgtcca atatttgacg aattcctcga tctcatcctc ttcgaaatcc acaatggtgc
    10381 attcgacaag gtcttcagca gaagggcgga cagcacgata ccccaccaca cggctggtta
    10441 agacaaactt attaccttca cgacgatgga aggcgaaaaa gtccaccaca cgctccacaa
    10501 ccgtattgcg caaactgata tcacgcactt catcgaggcc atccaataaa atgagggcgc
    10561 ggccagcctt caaggcttct ttcagcatcg ggccaacggg caggtctgca ccaatgccgt
    10621 caaaatattc tgcaataaaa tcatcaaggc gaacatcacg ggcggtgatg gcattggcaa
    10681 aagccgccaa cggaaggagg atgggcaggt aatcgttcag gccgatcttc ttcccttcgc
    10741 cgcgtgcaag gcgcaacgcc agatatttta caaaagtggt tttgcctgcc ccgggatcac
    10801 caaggatgat cacccccgaa tgtttttgca taactttcag cagcggcacc ggctcaccga
    10861 ggttgaacat ttcctcttct tcagcggaaa cgttacgtcc agccaggctc acatcacgct
    10921 tccaggtttc gccttcgggc aattccatgc gggccttcag cggaacataa agatcgagca
    10981 gtttaagctt gaggggaacg cggtcagaaa ctcccatccc tttcaggctg agatagtaat
    11041 gacgatccgt tagataagcg agatatgctg ctgtggcttc acgtaatttt tcgggaggca
    11101 gggcaggttt caggtcacgc cagaaacggt ctgcgagaac gatggtttcc ccatgaatga
    11161 tggcactatc ggttaccgat ccccctactg caatgctctg gtctcccgcc tgaatttcct
    11221 gctctggttg tgatggcttt gatgtctttt tagagggttg ctttttttcg gtcattgccg
    11281 cctcgttgat ttatcgcttt attccaaatc gagtaaacac cgcatggaaa ataattcaag
    11341 ctttccgttt accaatttat gcgatacttt gtaaaattca gtatacagta attcgaaaga
    11401 tttctgcttt accaaattgc aaggtgaccc actttattga cattttcact gttctgaata
    11461 taatcagcca aaatattgaa ggcgttgacg aggaaaagta ggcttgtacg acggttcaga
    11521 gagcaggtca gaacatggtg aaagacccgc ccgaaacaac aaagccgaat ggtcctccga
    11581 gctgcaaagg caaaaaatca gacagtttga gtagcctttg acggaatgct cccgttatag
    11641 agctagggcc tggtttttat aaaccgtgcc cgaaagagtg aggctggctt cccgttgcat
    11701 cataacggga ttttttgtaa ggtaatgcca ctatgtcagc ctaatttggg tggcaccacg
    11761 gatgcgagac catgcagcgt tcgtcccagt tgggatgaac gctgtgtttt tttaacccga
    11821 ccgaaggaga gaaaaatgaa ccccgtgacc gagcaagatg agcaaaccac atacatgcaa
    11881 gaacgtgcca ttgaaacccc cactgacgac tcccttcccg ccgcacaggt gatcgaagag
    11941 cagaataaag aagcagagca aaactatacg ccgcgcttta tcccataatt cattttttgg
    12001 aggaacccca tggaaaatac cacccgcttt cttttgaacg aaaccgacct gccaaagttc
    12061 tggtacaaca tcgcggcgga tagcccggtc gcaccgaacc cggttctgca tcctggaacc
    12121 ttggagcccg tcaccccgga ctttttgtct gtgcttttcc ccatggacct gatcatgcag
    12181 gagatcagca ccgaacgcta tattgagatc cctgaagagg tgcgcgaagt ttataaactc
    12241 taccgcccca ccccgctcct gcgtgcccgc aggttggaaa aggcgcttga taccccggcg
    12301 catatttatt acaaatatga aggcgtctcc ccttcaggaa gccataaacc aaataccgcc
    12361 atcgcacagg cctattacaa caaaattgct gggaccaagg cactgaccac tgaaacaggt
    12421 gcaggccaat ggggttcctc gcttgccttt gcctgcagtg tcttcaacat ggaacttgag
    12481 atctacatgg tgaaggtgtc gtatcaccaa aagccctatc gccgcaacct gatggaagta
    12541 tatggtgcca ccgtgtacgc cagcccaaca gaccgcacca attatggccg ctctgtgttg
    12601 gcagagaatc cagacaacaa aggctcgctt ggcatcgcca tttcggaagc tgttgaagcc
    12661 gcagccacct cgaatggcac aaagaaatac agcctggggt cggtcctcaa ccatgtgttg
    12721 ctgcatcaaa ccgtcatcgg tgaagaagcg ttgaaacaga tggatatggc aaacgaatat
    12781 ccagatgtgg tcatcggctg tgcaggcggc ggctcgaact ttgcaggcat tgccttcccc
    12841 ttcctgcgtg aaaacctgaa gaacggaaag cgcgcccgcc tcgttgcagt ggagccaaca
    12901 gccactccgt cactgaccaa gggtgttttc accttcgatt acggtgatac ggccaagatg
    12961 gcacccatcg tcaagatgca tacgctcggc cacgatttca tgccccctgg cattcatgcg
    13021 ggtggattgc gctatcatgg catggcgcca agtgtcagcg ctctggttga tgcgggacac
    13081 atcgatgcgg tagcggtcaa acagcgcgag accttcgaag ccgccatcca atttgccaat
    13141 gcagaaggca ttgtgcccgc ccctgaatct gcgcacgctg tccgtgcggc gatcaacgaa
    13201 gccatggatg ccaaagccaa aggagagaaa cgcgttattc tattcaacct ttccggacac
    13261 ggcaatttcg acatggccgc atacgaatcc tatctcggtg gaaaactcga agatttcgag
    13321 tatcctgccg aggcggtgca ggaagcgatg aagaaacttc cgcaagtgtc actctaatcc
    13381 gttcaaaaaa aaacatcccc atgacttttt gagtcatggg gatgttttcg ttttgcgttt
    13441 aactattgtg aacaggttcg ccaaatacca ctacaacaat atgttgatta agaaatttcg
    13501 gcgttacatt ttcgctggaa accacctgcc caagggtata tccacccgca atggggacgt
    13561 tcgtcccaag aatgtcgcgg actgcctcaa tctccacccc ggggtgtgct ttcaatagca
    13621 tctgccaggc aacatccacc agtacaagag caaagacagg ttttgaatca ccaagttgca
    13681 gcagggcttg ttgggtggca tcacgcgctg cacgttcaca agcggcacgg ctcccaacca
    13741 acaagtatgc atcgatccca tcacggatgg atgcattcat gcgaaaactt ccatcagcct
    13801 ctacgcggat gggagaacgc gtaatcaaat catcgcccac ttccacaccg agcggatata
    13861 gacgtgccag ataacttaaa ggagggaaag cccaatcacg ggccgggtat ccaaagagag
    13921 ccgcataggc ttctgacgct ggcctgccat ccaatgtccg cagccagaaa ccacgcgaac
    13981 gggtcactcg aaattgtttt ccaacttgat cccaaccatg gccataccca acaccggtct
    14041 tgatttcacc gcgtaagaaa gcggccacca taccgccggt acccgtttgt gtcccggtca
    14101 tttggtaggt attgccagta ttcaaatccc cgcttgagag tgctcccagt atgttcaaac
    14161 caggcggcat tgctccgcaa aattgttccg catctccatt gaaaccatct gcaaaaacca
    14221 ggagggactg acgctcctcc cgcgctgcgg catgatcagc caccttggca gccgtctcac
    14281 gaccggattg agcatacccc ggaagccaca tggcatctgc ttgaaaatcg ccgctcaaca
    14341 aagcaaccac tacagaatgc tgaaaatgcc cctcactggt taaccctgca ggtgaactaa
    14401 accctaccat gggaagctca cccagcaggc tggtgacgcc gttcaaaact tcacgcgcct
    14461 ggtattgatg ggatgccacc acgatggcaa agttggctga atttgcgcca aggtgattca
    14521 acgcctgatg cgccgcctgt aacccagcct cacgcccatc caatgcctgg gcagatccaa
    14581 cggatgcaaa caatgccata acgcctcgct actcttccgc aaccgggatg gtaatgtgga
    14641 atgtagtccc aacatggaat tcgctttcaa accatatact accgccttgc atctcgatca
    14701 ggttctttgt gatattcaat ccaagtcctg tgccggatgc cacttccttc gaatactcgg
    14761 tgcggaagta ttgctggaag acctttttct gatcttccgg ggtcatcccc aatccgttgt
    14821 ccttcaccca gatatgcacg acgcgcatgg aactgtttgt ctctccgaga ttactcgcct
    14881 cttctgcgcc aatctgcaag ataccgcttt caggcgtata tttgtaggag ttgctgagca
    14941 aatttacaag cacctgggta atgcggttgg ggtccgccca aatgacggga agtttttcgg
    15001 gcagcacaat ttccagggtt tgctttttct cttcgatctg gcgcttgaga gagcggactg
    15061 tatcatcaat caaaggcggt acttctgtca ctttataagc aaggctcaaa cgtcccgctt
    15121 cgatcttcga gatgtcgttg aggtcggaca ccaccgtatc catgcggatc acattggtct
    15181 tgatcgtatt gagaaaattc gcctgcatgt cactgattgg acctaccgct ccagccgcca
    15241 tcagatcggt gtaccccttg atggaagtca tgggattctt caattcatgt gccacaaatg
    15301 aaacaaatct gctcttctca aggttggcac gctgaacctc accaaacagc tgcgcattcg
    15361 aaatggcgat cgcggcatgg tcggtaagac gggttaggaa gccaatatcc acttgtgaat
    15421 cgctgatact ttccaaatga agaagaccga tcaccgtcgt ttcgcggcgg atcgggatga
    15481 tcatttgggt gtgcgcgctg gaaagcagtt tgtcgtttga ggtggcaaca gaaagacttt
    15541 gaggttgtcc attttcaatg gattggatca tggcaggcag ttcgattttc atcgcctgat
    15601 cttcagggag gttggccata cgatcatcca aaccctgatg agccatcacc cgcagtttgc
    15661 cttcttccaa cataccgatc aaccctgcct ctgcatccga ttgacgcaaa gcccactcca
    15721 gagtgatgtg catggcgcgg tccatttcaa gactggcatt caattcacgg tcaatgcgct
    15781 gcatgactga aagttcttcc acgcgggccg caagttcctg atcggtcagg gtgtataaac
    15841 gggcattttc gatcgccacc gccgcctgac ctgcaaacgc ggtcagcaaa tcctgatcgc
    15901 cggttacaaa tggaagtccg tcgcgacggt tgatcacttc aatgacgccg atgacgcggt
    15961 ctttcacctg cagaggcaca gccatcaaag aacggcttac aaatcctgtt tgttgatcca
    16021 caccacggaa tctcgaagcg gaacgatttt cttcattttc gatcacaggt ccgcgtgatt
    16081 gcaccgcacg acccacaatc cccgtccctg gcggcaggcg ttgaccaata aggtttgcag
    16141 ccaccgggcc tacggttact ttgaacacca attctttggt ctgttcatcc accaggaata
    16201 aactacccgc ttcacagttc aaaattccaa cagcattctc aaggatgttt tgaagcagcg
    16261 ggctcaactc aagcgttgag gtcaactggc ggttgatctc attcagcgtt gaaagttgat
    16321 gggcgcgttg ttgggtctct tccagcaaac gggctttgac gatcgcaccc gccgtctgat
    16381 cggcaatggc ttgcaaaagt tcaagttgtc cgcgcgtata agctgtagcc ccatcacggc
    16441 tgccgatgct gagcgcgccg atggattttg cacctgcatt gagagggacg cccatccagg
    16501 caaagacatt ttccacgatc ggggtaaggc tacgtgcctg acactcgtga atgtagtcct
    16561 gtgtcatgat ctggcggctt ttcgcgatca cttccggact aaggcctgct gtgaccggaa
    16621 acggcagatt ttctctttcg gaaatacgct cattattttc cacacaaaag ccatagtaat
    16681 aataatcgct ggcttcgtta tacaaagtaa tatgaaaatg tgaagtaggg atgatctgcg
    16741 cagtttgggc gtagatcagt tcaagtacat catcgaacgt gagggtcacg ttaacaccct
    16801 gcgaaacgcg ggtcagggca ttcatttcct ggatctgacg ctcaaggcgg gccacggtct
    16861 gcatacgctc aatcgccacc gaggcctgat cgcaaatatt ttcaaggaat ctcaagtcgc
    16921 gcggattgta aggctgcccc gataaacgcg gtccgagtgc aagccagcca ttgatgcggt
    16981 tcttgccggg taaaaccaca aagagacgcg cacccagcaa agccaggcgc gattgttctg
    17041 attgcaaatc cgatggaagt gcggcggttg tatccagata caaaggcaga tgctcttttt
    17101 cgaaataccg cacaaatgga ctggtcgttg taaaacgaat atcgcttgta gggcgacggt
    17161 cttcaccagg tagagcagag taaaagtcgt tgagagagtc gtatgtataa atgtgaaccc
    17221 tgctcggggt cagtgttgaa gcgatctgct cgcgcaggat cgtgctgatc gagtttagat
    17281 ccagcgcagt ggcaagcttg tgggaaaaat cttcaagttt ttcagccagc gcacgctcac
    17341 ctcggaagaa ggtgttatcc accacagatt gcaaacgcac acgaaaaggc tccagcaata
    17401 aagccagcaa caacataaac ccacctacaa ggtagggatt gctcacaagc gcatcagaac
    17461 tgctggagtt aactaaaagc cccaccccgg ccaccaccaa cccatacccg cccatgacaa
    17521 atgcggtcag gaaaaagtac atcagtccgc gccgcacaaa atcgtcagtg cgcaggaagc
    17581 ggaaacgtaa aatggtatag ccaatggtca gcggaaagaa agcaagcgga agaaacaggt
    17641 agggtgaaaa attcaaaggc tgggatgcag atgagccagt ctctttccac agcagttgaa
    17701 aagcattcaa gccaagccaa atgcctgccg gaacaaacgc cagcatggct cctaaaagga
    17761 tggcacgcgc ttgagttttc acaactggag attgtgcaga aacagcctga tacaggctaa
    17821 ggccaatgta aaaaataatg ctaatggcat tgaagccata aatgtattgc cagaccggaa
    17881 tataggctgt gggtctttca aaattgaaca aggttgtgag gttgaacaca gccaacacaa
    17941 ttccaataat aagcccaacc cagcgcaagt atggacgatt aatcaccaca cgtggttcaa
    18001 gcgggaagga aaggcccagg tcaaatagag cgccgcccat gatcgccaga gcgaaagtcc
    18061 agaaaactgt aaattcgtgg gtggtgacca ggttgaaata tgcccccgtc acaatggcaa
    18121 gggatgaagt aaaaagagaa aaagcgcgtc ctgccggttc attgcggcgc aaaccaaaga
    18181 tccaccagct ggcaatgata aaaacaagac ctagaatgga agggacaata aaatacgtgg
    18241 tacggctgga ggcagggaaa ttatgtaaag tgacacttac atcacgagac tgacccgatt
    18301 ctgatttcac cgttacagtg acattttcac cgggcacatg cccctgcaaa atggaacgaa
    18361 cctcacgtgt attacgcact ggcgttccgt caattgcaac catctgatcg ccaactacaa
    18421 cctgcttgaa taaagcccac tccggcattg cctcacttgg gcctgtgcca ttgaacacca
    18481 tggtatgttc atacaatgcc cccaaaaatg gctgttgaaa ccaccgaacc gacaggtaca
    18541 tgccaaccaa aaaaataatg acagcaatcg cctgatatgc aaacacagtc cattgcagga
    18601 tctggttgaa gagggtgcca ttccgcggaa ggttgatctg cgcactgcgc tgaaaatttc
    18661 gagccgccat agtgaacgaa ttttactcta caacggcttt gacagtatat ggtactttca
    18721 taacaaagaa gaaaggctga taagaaaaga gaccgctaaa tgcggcctct ttttgaaaga
    18781 tcacttcttt aattgagatt aaaacacccg cttcaccccg accccgaaac gggtacgcta
    18841 aatatcgaga ttcctcacgc tcttggcatg ggtctgaata aatttcttgc gtggatccac
    18901 cgcatcaccc atgagcatat caaaggtgcg gtctgcttca gcggcatcat ccacagtgac
    18961 aagaagcagg gtgcggttgg tgggattcat ggtcgtctcc cacagctgtt cgggattcat
    19021 ttcacccaaa cccttataac gttgaatgcc aaccttatcg ctgccgatct ctttgaccaa
    19081 tttatctttt tccttgtcgc tatacgcata cttgacctga ttcttataag ccacacgata
    19141 tagcggaggt tgagcaatat acaaatgccc ttcgtcgatc aaaagcggca tatagcggaa
    19201 gaagaaggtc aacaacaaag tgcggatatg gcttccatct acatcggcat cggtcatgat
    19261 aatgacacga ccataacgca aaccttcaag gtcaaagtta tcacccaccc cggttcctaa
    19321 agcagaaata agggagcgga cttcattgtt acctaaaatt ttatcaaggc gggcgcgttc
    19381 ggtgttgagg atcttaccgc gcaatgggag gatcgcctga aagtggcggt cacgtccttg
    19441 cttggcggag ccacctgccg agtcaccttc cacgatgtaa agctcggtct tggaagaatc
    19501 acgttcagag caatcggcca atttaccggg cagtgtcagc gattcaagtg ccgatttgcg
    19561 aataacaaga tcacgcgcct tacgggctgc atcgcgtgcg cgcgccgaag tgagacattt
    19621 tgcaatgatg gccttcgccg cctgcggatt ttcttcgagg aaggtgccaa aagtttcgcc
    19681 gaccacctga gtcacatagg tctgtgcctc gggattcatc agcttgacct tggtctgcga
    19741 ttcaaattgt ggatcggggt gtttgaccga aacgatggcc gttaaccctt cacgagtatc
    19801 atcgccagag aaattcgggt cggcatcctt gagcaggcca ttcttacggg cataatcgtt
    19861 gatgacgcgt gtcagcgagg aacgcagccc cgtcaaatga gtaccgccat ccacagtatt
    19921 gatggtattg gcgaacgaat acaccgattc ggtgtaggca tctgtatatt gaatggcggc
    19981 ttcaataccg atgttctcga tctctttttc cacatgtacc acagggtgca ggttttcacg
    20041 gttgcgattc aaatagcgga caaatgaagt cagaccgcct tcaaagtaga aggtcatttc
    20101 acggtcagca cgctcatcca caaacttgat ggtgatgcca cgggccacaa aagacatttc
    20161 acggaaacgc tgaaccaatg tatcgaagcg gaaatcaata tcttccgtaa aaatctgttt
    20221 atcaaagcgg aaggtttgct tggtgccggt ctcgtccttc actttgccga tctgtttgat
    20281 ggatccttgt ggcacgccac gcttatattc ctggcgccac aatttgccat ctcgtttgat
    20341 ctcggtaacc atccattcag agagggcgtt cacagcactc acacccacgc cgtgcaaacc
    20401 gccagagacc ttgtacccgc caccgccaaa tttaccgccc gcaccgatca cggtcatgac
    20461 cacatccaac gtttccattg gtttgccgtt cgcatctttt ttggaggggt gcgggcctac
    20521 aggaatacca cggccattgt cttcaatggt cacactgcta tcggggtgaa tggtgatatt
    20581 gattctgttg cagaccccag ccagcgcttc gtcgatggag ttatccacca cttcatatac
    20641 gaggtgatgc aacgctttga tatcggtacc gcccacatac ataccggggc gcttccgcac
    20701 atgctcgatt ccttcaagtg cctgaatgga actaacatca taattaattt tcttttcttt
    20761 ttcagccatt acaacctcca acaattttta ttgcagaacg gaatgccgtc gaactatgcc
    20821 ttcgtaacgg cattcggacg taatttctcg atcacagcct gtaaccaggc cgtcatatca
    20881 cgataataga caaaactcga agccagcaat gccgtggtca caaatgagtg accaaagatg
    20941 ccaagaaggg tcatccacga atcttcgggg gggatgctcc acagaaaatt caacccaacg
    21001 gacaaaagga aaacggtcaa tacgaacatg ctgctggtgg gcagggtgaa gcgtgtcatc
    21061 tgcacgctgc tcaaaatgga agtgaggaag ttctgacgcc tgagaaaaat cccatgcggc
    21121 cagaaaaaga aaggcacgat cacccacatc gaaatgaggc tcatgaacag gatcagcagg
    21181 ttggcaaaaa aggcgctaaa ttgcaaaacc actgccagca ccaaaaaggc aggcaccaaa
    21241 ataatcacgg agagaatgct ccagatgatc gaagtcagca ccgtttgtac aatggcacga
    21301 gaaatctgaa tgggctgatt ttctttatcg gcaacggtca gccaggcaac actgcgaaaa
    21361 tacaagccgc ctccaaccca cccaagcagg ttgagcagaa aaacccagcc catcaggttg
    21421 acagcatcca cctgtaaaac cagtggattt cccaatggtg tggcggctgg ctgttgtgga
    21481 aataggaggc ttgaaatccc gatgggaagc gttcgaacga tccaaaacag gttgatgttc
    21541 ggaatcgtcg tctcatacca ggtgaggatg cgttgaatat cctccgcagg gacaccaccg
    21601 gtgcgccaga tcgacacgac gtcatccttg atcgagttga acagggcatc catgcgcagg
    21661 cgtggaccaa gccacaggaa cagattcaac aacagcggca atagaatggc cgtgatgtgc
    21721 gaggcgatca tatcgaagcc cgctttgatt gaattgatga ttccaggggg cgtgggcaga
    21781 ttttctgtac gtgaaacttc cataacaggt tgattctacc atacccctgc ttgcgcttgc
    21841 gtgcccccac aggtacaatc ttcacatgca agagcgccat tcctacctgc catcagcgga
    21901 ccgtgtgggc gtattgatcg cctcggtcct gctgactttt gccctcaccc gcctcattca
    21961 gtcgccgcaa tttacgttga cgatcgaact gcctggcttt tattttgcat atccgctcac
    22021 ccttggcaca gccatgacca tcctcgctgc tgcactggcc gccgcaggca tggattggtt
    22081 gactcgtggt caccccaacc ttggggaaaa accgaacatc gaacatctca tgctgcccac
    22141 cctcactacg tttgtgatcg gctcgccgct cgcgctgctg ccggaaagca ttgcctggtg
    22201 ggtggcgctg gcatttgcag caagcctgct ggtgatcgtg ttcctggcag agtacatcac
    22261 cattgaccca tccgcgccct catatgcgtt cgctcgagcc ggtctgacag cactggccta
    22321 cgccctgttt ctcatccttg ccatgtcgct gcgtttttcc ggggtgcgca tgttcctgct
    22381 tttcccggtg gtgttggttg tggcagggct gatcagcctg cggattttgc atcttgatgg
    22441 cactgaccgt tgggattttc cgtgggcgct tggcattggc attgtctgcg cgcagatcgg
    22501 agcagggttg cattactggc cgctgactcc gatacagttt gggcttgcac tcacgggtcc
    22561 gctgtatgca ttgacgatgc tatctgccag tttgaccgag aatacgccgt tacgccgtgc
    22621 cgcagtaggg ccgatcttaa ttttaggcct gagctgggtt tcggcttttt tagtgaaatg
    22681 accaccacac atttcaaatg aactccctca ccctgacaaa tgaacaactt caaaaaatga
    22741 tcgaccatgt ggattcccat gcgccgctgg aggcatgcgg tcttttggca gggaaaggct
    22801 ccacggtgga ggctgtgctt gttgtgcgga atcaggccca aagtccagtg cgatacgtga
    22861 tggatcccat tgagcagtta catgcctttg agtggattga gtccaacggg ttggatctca
    22921 tcggaatctt tcattcgcat ccaactggac ctgaaactgt ttcgcccacc gacatagcag
    22981 aggccgccta tgcagtgacc tacgtcatcc tcgcacgcgt gaatgatgca tggcgcaccc
    23041 gcggattttg ggtggagcat ggcggttttc gtgaagtgga tttgcaaatc ctctagcaac
    23101 cttatttcat tttttgtgtt ttatgattac aacctaattg gaggagtttc aatgcaaatt
    23161 gtgtttgata tcgcgcttat tctgctggct tatgtttttg gttcgatccc ctttgggctt
    23221 ttgatcgtga agctaaaaac tggaaaagac attcgtgagg tggaaagtgg ccgcactggc
    23281 ggcaccaacg cgatgcgtgc ggcaggtttt tgggcggggt ttgcaaccgc catgctcgat
    23341 attttgaagg gtgcagttgg tgtgtgggtg gctcgctggc taagcccaga ccaaacctgg
    23401 gtgcatgtgc tggcgccgat cgcagctatt ctggggcaca attattctgt ctttctcatt
    23461 gaacgcgatg aaaatggaaa gatcatccgc tttcgcggtg gtgcaggtgg ggcgccttcg
    23521 gtgggcggcg ccatggggtt gtggcctggt tcgatcctca tcattttgcc actcggcatg
    23581 ttgacctttt tcagcatcgg cattgcctcg atcacaacga tggcggtggc attgttcgcc
    23641 attatcgttt ttgcgatccg cgcttcgcag ggacttttgc catgggctta tgtgtggtac
    23701 ggggtcggcg cggaacttct gctgatctgg gcgttgcggc caaatttgaa aaaattattt
    23761 gaaggcaatg aacgcgtggt gaagtacagt ttgaacggtt ggctgcgggc aaggcgtgag
    23821 gcgaagacag cggctggaaa gtagaaagtg gaaggtagac actggaatca aaaacgtcct
    23881 tttctgaagg gcgttttttg ttgcaagggg tatgctatac tttttatcgc ctactgggaa
    23941 ataatcggac taattttatt caaggaatga acaagatgaa atccattgac caaaatgata
    24001 tcagtctccg tcgctggtca aaaaacgacc tgcctttgct tgaggggttg ttgggcgacc
    24061 cagccatgac caaacacatc ggcggaccag agactcctga aaaaatccgc gccaggcttg
    24121 agcgatattg tgtagatagc agcatcagca agatacacat gttcgtcatt gtgctgcaag
    24181 ccaaacaatt gggcatcggt tcgatcggat tttgggaaag gcaatggaag aaccagacag
    24241 tgtgggaaag cggctggcgc atccttccag cctatcaagg caggggaatt gcctcaaagg
    24301 ccattgcttt ggtcatggaa cgtgcccgtg ccgagcagaa gcatagatat gttcacgcct
    24361 tcccatcggt tgaaaatgac tcgtccaatg ctttgtgcaa gaaaacaggc tttgttctat
    24421 tggaagaagt tgattttgaa tatcctccgg gccatacgat gcgcagcaat gactggcagc
    24481 tggatctctt ttcgcatatg tctgaagatt gaagcgtgta ccgaactggg tcagcttctg
    24541 aaaagcacaa atttatcctt caagacgata aacgagcagt accgtttctt acaccactgc
    24601 tacttcccta gtgaagcgaa tgtaaaactg tgaatgacaa cttcaacttc atttccatcc
    24661 acgggcggtt gaccttgaaa cagccacagg ttgatgtgaa cgggcatggc ttgctgggaa
    24721 atgtgaacca agggttcatc tggtttgtac agccaattga cgatctcgtt ttcattgtca
    24781 tcacggtgac cgtgcagcgc ttgaaaaaga atcttctggc tttcccacac aaagcgatga
    24841 gtggtatatg acccgttcaa tgccacaggg aaggattctg aggtttgctc cacaccgctt
    24901 tgggcgggcc agaccgtgaa gtttccgatc ggccattcgg ggttgcccca atgggcatat
    24961 tcgatgtcaa tttcattggt ggcattggga ccaacatctt cagttgggta gttgaacatg
    25021 ccaaagacta cgttggcatc caactggtca atcggcccga tgacttgaaa ctggtagatg
    25081 ccaaatccaa gccgctcggt ggttgtgacc tctgcacagg tccagcccga ttcggtgtgg
    25141 gtgatcttca aatgcaggtc accgttttca tccacccaaa cattgttccc attccaggta
    25201 tttggcccgg gtccgctcag gccgccatcg cggacgatcc aatcgtagcc agaaaattga
    25261 atgatctgct ccccagttac aacaggcgga gttggagatt cagtgggaag cgaggcaggc
    25321 ggggttgaat tgcacgagct caacacggca ataagaatga tgaatgcgat tctcttcata
    25381 ccccgtgtcc accagccact ttcaactctg agccgctggg agcaccgccc attaacggca
    25441 tggcttccgc aatcaacgct ctgacatgct catctttcag cgaggcatca tgcgcttcct
    25501 tgtcatccca aatttccatg acccagaccg ttacttcatc agccaggtct tctgtaacgg
    25561 cgtataaatg acaactcgag agttgaccaa ccatctgcgc ggcgcgcaag aggatctcga
    25621 cgagttgccc acgttggttg ggtttggcga tcaatcttcc acacattgca tacatttttt
    25681 tactcctgat gttaaagcca gcaatcgcat tgcaaataac gcgagggcca ttttaaacct
    25741 agtggacacc tattcaaatc aaaaccaaag tataagccaa atacaatata accagcaaca
    25801 atgccattta ttaagggttg aaatattgaa ttgacatggc gctgtatgct acactcagcc
    25861 ttaatcgcca aaggagatgc ctgacatatt attttcaaac tgcaaccacc cccacgagca
    25921 cgcaaacctt atcaccaact caggagaaaa caaaatgcgt aagtcagtat atctggtcgt
    25981 gaccgttgca atcttgttcg ccttgcttac taccgttaca ggtgccgccc aggctgcccc
    26041 cgctgcacaa gccaagtgga ctgtgatggt ttacatttcg ggagataaca atctcgaaga
    26101 ttatgttgta aaagatttgg aactcgaact cgcacccacc gggtccagcg ccaacgtaca
    26161 agtggttgca cttgcagacc gtggtcccgg ctatgacatc agccgcggag actggcagac
    26221 caccaagtta ttccatgtca ccactggcat gatcgccgat tctgccagcg ccgttgcaga
    26281 ttggggtgaa cgcaacatgg gcgataaaca aacgcttatt gatttcgttt catggtcaaa
    26341 gaccaattac cctgccgacc attatgcgct ctatttctgg ggacacggct ggaattggca
    26401 ccccggttac gtgatggaag atgtcacaaa tgccgataca cttgacctcg acgaattgaa
    26461 agcggccctc ccctcactcg gcttcattga cgttgtcggc tacgacggat gcaacatggc
    26521 gtccattgag gttttcaacg cctggcatgg gtatgcaacc gccgtctcca gttcacagga
    26581 atatgtcggc tgggatggca ttgaatatga tgtcgtcctc gcacaactgg cagccaaccc
    26641 aaacatgacc gccgaccagg tagcgatcgc cacgagtcag agcgcatcca acgacaagac
    26701 ctggtctgct gtggccgtgg acagccgctt caacgcctta tacacagccg tggatcaatg
    26761 gtcagttgcc ttgaagaacg gacttgccgc caaccgcaaa aaatatgacc gcgcttttgg
    26821 cgccacccag gatttctggc aggctccaat ggacaaagac ttgtacgaca tggcctacga
    26881 gatcaagcgc aacgtagccg ataccaacat caagaacaag agtcaggctg tgatggatgc
    26941 cattgcaggc gtggtactga acgagcacca cgtcaacggg tatccggacg cacatggcat
    27001 caccatctat tacgtttcca gcgccgcagg caaggactca aattacacct actatcgttc
    27061 cacggtcgat ttcgctttga ccaccggttg ggatgagttc ctcgacgcat acgccaaatg
    27121 atcacttcgg caagttaata caatcaggcc tccagaaatc tggaggcctg aattttttcg
    27181 accaacccaa aactagcgcg attgcgggct gtgattcgga agcgcccttg gctgacgggg
    27241 caacccacag tgcgggcagt tcatcaatcg gatggaaacc gtgtaatcac gtggacactc
    27301 gcaatacttc tttttctcca cccgctttcc ttcatggtca acccacttac cggtatcatc
    27361 caattcctgg cgcttatggc acatctcaca ccaacggcca cattcgtcac tggtataaac
    27421 ccatttatga agaccaactt tgcaaaattt catagtacct tcctctggcg gaccattcgc
    27481 ccaacggttt acgctaactg acagttagac ggactcggcg cgcccatcgc aaaggggatg
    27541 agccgcagcc aaggccgtag tataaaccaa tttttacagg ctgttcccat ttctttcatt
    27601 ttttcaaggt gggtcatccc tttacatagg acaatttttg cgagatcaaa ccgtccttta
    27661 actgaaaaat atccacacca cgtacgtgcc ccttgttccc agtcacatcc acccaactgt
    27721 atttccaacg catgacacaa cggattccat acccgaagat ctcttcgatc tcaatatgag
    27781 cctgaggcga ctcgcgaaag aaatcctgcc agaattgcgt aaccgcctcc ctgcccgaat
    27841 agaccgtccc atccggggct gggctggtat tctcaaaaac acaatcatcg cccatcaacc
    27901 gcatcatccc ttccacatca tgacggttga aggcttcatt gaattcgatc acaacacgca
    27961 cggctgattc cacttttgac attcgcacag ggcacatggc aggacctcct acatataaaa
    28021 cgcataagca ttaaaacgct gttacgatta taccgacaat tgcggcaacg atccccagtc
    28081 cctgaatctt gttgatctta tccttcaaga aaataatggc cagggtgatc gtcacaattg
    28141 acaatgccga agcaataggc gtgatcaaaa ttgcatctcc aatggtcaag ccgtaattta
    28201 cgagcgccac tgcaccagcc tcaatcaccc ccatgaggaa aatgatggct ttcgtgttcg
    28261 cggaaacgtg agtcaggccg atcccctgtt ttgtcaaaaa cgaaaagatg gacaggaata
    28321 tgataatccc tgctttgatc aacagggttg tcaataacca tccgaccttt tcagaaataa
    28381 tctcgctgac attccaaaaa acgccaaaga aaaaagcacc caaaatagct tccctgaccc
    28441 ctgccgaaag ttgaaacttt cggtttccaa tttcattcca attcaaagac gccacagtgg
    28501 cgccacaaat aatcatcaag acgcccaggg ttttcaaagc agagagtcgc tgccccatga
    28561 aaacgaaagc gaacaacatg gtaaacacag cccataggtt catggtcgct gcgatgatcg
    28621 aaacattacc tgtctcaaag cctttaaaga aaaataaata tccagcagaa taaagaattg
    28681 ccgcgatcag ggatagcgca accaccaaaa tagggatgtc aacggtcagc ctggcgacca
    28741 cggcaaggat caaaacggat atcaaacccg ccaattgtga ccaaaacaaa gacctgaaag
    28801 acccgatctg tttggcaaac acaccgccta aaaagtcata tattccccac ccaaacatac
    28861 cgccgatacc caataaaatg ctcaagatga ttgtattcat gactcttttt tacccgtgaa
    28921 atgtccaagc ccatctgttg agattacgaa tacactgcac atacacttct aatacttact
    28981 acttcagtcc gttgaattgc cagtgatgac aacgcagcga ttggttttcg agtcgtgcaa
    29041 cagatatgca aaacgccaga aaatgtaatg accgggaagg ccaacatcac ttccataata
    29101 atagagagtc gacgtttcaa gcggcacaag cagattgagg tggcgccagt attccgaaag
    29161 aagtgcatcg atcaattgcg gcccggcaga acctccacct acatatctga aacattccgt
    29221 aacgtactca acaagctcat caggctgggc ggaccgtatt tttgcttcaa agggggtcga
    29281 tttcggaaaa aacgtcatca tcaactcagg gatcggcagg tcagatgcgg gaactgtcgc
    29341 agcatggacg gaaagagttg tcacatagtt gaggccgcac agagttgcgt caatatggga
    29401 gagcaatgcc cgaaattcac gcaaggcctc atcaggcagg ggcttgatct gctgcaaaac
    29461 ccacggctgc ggtaatggtg aagctttttg cgctgtctga aattgtgagg gccgcgtcat
    29521 gcgcagatat gcaaggcggt catccacaaa gatttttgct tctgcaagtt gaacacccgt
    29581 aaactgacgc agttctctta ccgccgcaac aagattcccg tcaagtagtt gtttatcaat
    29641 gatcgcaagt tggtcaacag ttaaattcac cgaggtttcg ccctttgtat gatttgatcg
    29701 tattattccc attttagtca ttcagggtgt tttccaacac cctgaatgtt cgtggtcttc
    29761 gttacttccc gtatcgttta cggatctctg gcaggaaata ctcgtcgaaa aacttatcca
    29821 catcgtaatc cggcgtccag ttccaatctt tgcgggcgcg ggaatcatcc acatcctctg
    29881 gccacgaatc gacgatcccc tgtcggcgcg ggttgggttc aaagttgatt ttcgcatcgg
    29941 gaaaagcctt caacgcccgc tgacggaatt cctctgcggt gagtgcgaac gctgcaatgt
    30001 tgtacaccgt attggacagt ttctcgcgcg gcacatccat cagcatcaac aaagacttga
    30061 tcgcatcagg catggccata aaagagatct tcgtatcggc acgcacaaag caggaatatg
    30121 gcttcccttg cgccgccgca tgcagcatct cggggccgta atcgcttgtg ccgccgctcg
    30181 gcaatgtaaa agcgctgatc aagcccggga agcggatcgc ccgaaagtcc agcatgaccg
    30241 gtggtttttc atccaaatgt ttctgaccgt agaattttcc gtaatacatc cccagtttct
    30301 cacaatacaa cttgttgcat ccgtacatcg tgtgtggata atcccactca tcttccttca
    30361 ccaaccccgc ttttcttttt gcatccagcg ttggcatgcc atacgccgca atcgaacttg
    30421 ggaacaagaa cttgacagcc ttgccatact tttccgaacg atacgccgcc agcatcaaca
    30481 actgcattgt gccttccacg ttgatgcggt gcgcctcctc cgttgccact tcagccttgg
    30541 aagaaagcga agccgccaga tggaagatga tatcgaaatc gtaatcgtaa aaggtcttga
    30601 tcttgtaaac cagatccccc tgcacatgtt cggccgataa atgtttgatc gaatccggca
    30661 gcggcataaa atctgaagtt acgatccgat acccgcctct tgccgccaac ccctgcacca
    30721 gcgcctgccc aatttcacca gatgcaccgg ttacaagaac ttgcttatcg ctcattgtca
    30781 actccttatt atgctagaat gaagttatct tattttacgc cacaatcaaa atttccctgt
    30841 cataaattct ccatgatgtt tttacgtttt caaattcccg ccaaaaaatt cctattgacc
    30901 gccctcacca ccctgatgct gaccagttcc tgcgccgtgg tggatgcgat gctcaccacc
    30961 cctacaccgc aaatcccgac ggaaaccccg attcccactg cgaccatagt ctggttccca
    31021 ccatcggcca ctccctccgc ccaaccattt tccacccaac tccccacccc cgaaatgcgc
    31081 cccggccttg gcagcattgt cctcactgac aacttctcaa acgaagaaga ttggaacaca
    31141 gccacatctg gccaggccag cgccgccatt gaaaacaatc aactcatcct cgcatcacag
    31201 agcaaggtat acatgctcag cctgcgccgt gaccttgccc tcgacgatta ctatgccgaa
    31261 atcacggccc gcatcaacct ctgccgcggt gacgatagtt atggcatcct cgtccgcgcc
    31321 aacgcagtcg cttattaccg ctttgcgctg acttgcaaca gcaccgtcag tgcggaaaga
    31381 gtcagtgtag gcacacgcca gttattgcaa tctcccctgc ccagcggaga tgctcccccc
    31441 ggagccccgg gcgaagtgcg tatcggcatt tgggctgtcg gcacagaaat gcggctgttc
    31501 ttgaacggac gctaccaatt cagcatcagc gacacgaact accccagcgg caccatcggt
    31561 gttttcgtca actccgcagg agatactggc acggtcattt catttactga actaacggtt
    31621 cgccaggtca acgccgctct ccctgcagaa tcatctccgt aaatcacaca ggtttaaaag
    31681 aaaaatccgc aaaactctgc ggatttattt cttcacctta tctctggtca taaannnnnn
    31741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgatct gcccgatttt cggcgatctg
    31861 ccgcaacgca gggatggcac gttcatcgcg caagtttgca agtgatgcca ttgccttctt
    31921 ttgcacaacg cgatcgggat catggagcat ttcgatcaat acaggtgcta cgcgcacatc
    31981 ttgaattttt cccagcgcgg tcaccgcatg ttcgcgtatg gcggcttcgg ggtgattcaa
    32041 agactcgatc aaaatatcca cagcctgcga cccaaatttt gtcaacgcct cggcggcggc
    32101 ttcacccact tcatggtgca ggtcgtataa ggccatgccc aactcttcaa tggcgcgaat
    32161 atcccccaac ttcgagagaa tcatcgccgc aaacttgcgg accgtccctt ccttatcggc
    32221 aagtgcatcg atcaatggat acaccaccgc ttcgcccata ttctcaatgg cggtcaacag
    32281 cacagaaacg gcctgctcct tttcatacca ccagtaggaa tcgcgcagcg cttccataag
    32341 aaaagggatc gcagcaggat gacgggtatc tcccagggct ttcgcggcgg cctgtcgcac
    32401 ctcgatcttc aaatcgtcga gcaagacatt ggtaatctca tcaaaagtgg aagggtcgcg
    32461 gaattttgca acagccttgc aggctgcaat gcggacttcg tcctcggggt ctttgagcaa
    32521 gggcaacaaa tctgaaacgg ttctttcttc accgatggct gcgagggccc aaagggcttt
    32581 tgcccgcacc gtaaaatatt cgctcttcaa cgcatccagc aggacgggta tgagcgcctt
    32641 atctttgctg aagccaaaaa cttcgatgat ccgtccgcgg atgacgggat gcgcggtgga
    32701 aagtaccttg atcaattgag gttgggcagg agatattcgg gcgagaagat gctgatagag
    32761 cagaagcagg ttcaagtctt gggtttgaag cgcttccatc agggccggca cggcatcggc
    32821 gccgagtttc aagaggcttt gtgaggcggc atcacgtttc gtcgaatccg caagctgcga
    32881 aatcaacttc cttgcttctc cctgtttcga acctgtgagc cagtccatgt ttttattcta
    32941 atctaaaatt tatcagtgaa gaaaagttca aaggaagtat ctttcaaaaa ctctcaggcc
    33001 agaaatcggc caatttttct ctgatataat ccccgattcg atttgcggga atcataacgc
    33061 cagagggctt ttggtgttct tttcctaagc atcacactaa ttttcagtgc gctttggcgc
    33121 acgataaacc ttatcggagt tcccaactaa tgaagaaaga acaacagtta gacctctatt
    33181 accagatggt actgatccgt cgtgcagaag aacgcggcgc ggagttgtac cagcagggca
    33241 aaatcggcgg cttcatgcat ctttatatcg gtcaggaagc cgtttcgact ggtttgatcg
    33301 cggcacgtga accacgtgac cgtgtcatca ctgcctaccg tgaccatggc gtggccatca
    33361 attgcggcat ttccaccaac gaagtaatgg cggaactgct cggcaaggct acgggcatgt
    33421 cgaagggcaa gggcggttca atgcacatgg caagcaccga gaagaacatg tggggcggac
    33481 acgccatcgt cggcggccac ctccctgttg cggcaggact cgcccttggc gaccagtacg
    33541 ccaagaacga caacatcacc atctgcatgt ttggtgatgg cgccaccaac atcggctact
    33601 tccatgaagc cttgaacctc gccaagacat ggggattgcg ggtgttgtgg gtttgtgaaa
    33661 acaaccaata cggcatgggg accgcggttg agcgcgcctc ggctgtgacc gagatccgcc
    33721 aaaaggctga aggctatggc atgaaaaacg gccaggtgga cggcatggat gtgatgaagg
    33781 tttacgaagc cgcaaaagaa gccttggact ttatccgcaa agagggccag ccctacctgc
    33841 ttgaaattga cacctaccgc ttccgcggtc actccatggg cgaccccgaa cgctaccgca
    33901 aagctgaaga agtgaagcag tggcaggaaa atgaccccat cggcatctac aacaagtatc
    33961 tgcttgataa aaagatcgcc acccgcaagc aattggatga aatcgaatcg cgcgcggacg
    34021 aagaagtgaa caaggctgtt gagtttgcag aagccagccc cgaacccaca atggaagacc
    34081 tgtacgcgga tatttacgcg gataactaaa accactaaga cacaaagtca cggaggcaca
    34141 aagaagtaaa aatcttggtg tcttggggtc ttcgtggctt cgtggttaag gaataacaca
    34201 atggcaaaaa ttacaatgcg cgaggcgatc tcgcaagctt taatggaaga aatggaccgc
    34261 gaccccgccg tctttatcat gggcgaggaa gtcggtgttt ggggtggcac ttatgccgtc
    34321 accaaaggtt tttttgacaa gtacggtgca gaccgcatca aagacacccc cattgcagaa
    34381 aacgccatcc tcggtgcggc catcggcgcg gcgatggttg gtcagcgccc gatcgctgag
    34441 ttgatgacca tcaacttcgc cttctcggca atggactaca tcgtcaatca agcggccaaa
    34501 ctgcgctaca tgttcggtgg acagttcatg ttgccgctcg tcatccgtgc cgtcggcggc
    34561 ggcggacgcc aacttggcgc tacccactcg cagactcccg acgccatctt cgcgcacttc
    34621 cccggtctga aagtcgtcag ccctggaacg ccagaagatg ccaagggtct tttgaaatcc
    34681 gctattcggt cgaatgatcc gattctcttc atcgaacacg ccaccatgta ccaggtccgc
    34741 ggcgaagttc ccgaaggtga atacaccatc ccgattggca aatccaaaat tcagcgcccc
    34801 ggcaaagacc tcaccatcgt cacgtactgc aaaggtcttg aactctccat gaaggcggca
    34861 gatgaactca gcaagcaagg catcgaagcc gagatcgttg acctgcgcac cctgcgcccg
    34921 ctcgatatgg aacccgtgct cgaatccttc aagaaaacca accgcgccat cgtcgttgaa
    34981 gaaggctgga agtcttacgg cgtcggcagc gaaatcgtca gccgcatcta cgaagaagcc
    35041 ttcgattatg tagacgcacc gatcatccgc gtagcacaaa aagaagtgcc cctgccttac
    35101 aaccgcaccc tcgaacaagc cgcactcccg caagttcacg acatcgtcgc cgcggcaaag
    35161 gaggtcttga aataatggct gaaacaatca acatgcccaa acttggcttc gacatggcgg
    35221 aaggtcttct cgtccgctgg gtgaagcaag ttggcgaaaa tatcaataag ggcgatgtcc
    35281 tcgccgagat cgaaaccgac aaagccaccg tcgaagtcga atcttccgcc agcggtgttg
    35341 tgctctcaca catcgtcgat caaggcagca tggtgcccgt caatgcaccg attgccgtcg
    35401 ttggcgctgc cggtgaaaaa gtggatgcgc ctgcggcggc tccaaaagca gaagccaaag
    35461 cggaagaaaa acctgcggct cagactcagc cgacagctgc ttccgctccc tctgggcagg
    35521 cctctgcccc cgctgacaat ggtcccgtga aagcatctcc gcttgcgcgc aaagttgcca
    35581 aagataacgg tgtcaacctc gcctccgttc agggcaccgg tcccggcgga cgtgtggtac
    35641 gcaaagatgt agaagccgct ctcgcgggtg gtcaaacgtc aaaggtcgaa agtcgcccat
    35701 ccgccccaat taccaattac caattacctg tctccaccga agataaagtc acccccacca
    35761 ccaaactacg ccaagccatt ggtcgccgac tcgtcgaatc caagcagacc a
//
LOCUS       DAOQJC010000030        35677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_528_length_35677_cov_8.030487, whole genome shotgun sequence.
ACCESSION   DAOQJC010000030 DAOQJC010000000
VERSION     DAOQJC010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 35677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35677
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_528_length_35677_cov_8.030487"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PLE39_06275"
     CDS             complement(<1..697)
                     /locus_tag="PLE39_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE67967.1"
                     /translation="MTNTNATQNEFLYRMSNSLRRGLADVQKNVNDLYRLEELEDYIG
                     VKDSPMEYIQAALAPMIAQVSDFQDYAETVTGRMALNLEEVDIHALLDGVLTIADQLV
                     EQKSGVTLEDEIEDDLPLIEADATRLSQALLNLIHNAVKFTESGTVTVLVSREPEDIL
                     FEVRDTGMGIAEENQELVFQPFETILEDKDDPRLGFGLGLKIVEHIVDLHDGETWFES
                     DLGSGSSFFFTIPL"
     gene            complement(704..>2473)
                     /locus_tag="PLE39_06280"
     CDS             complement(704..>2473)
                     /locus_tag="PLE39_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE67968.1"
                     /translation="YVTQSEEFYDKPLDPNIAKRLIGFVAPYQWQLLFSAFLMLVAVL
                     SSVMGPYLVKIAIDDGLVAGNYTVLRNVVLFYLAAAIIRWGFIYWRVNIMARVGQSVI
                     YDMRKLLFEHLQSLSLGFFSRYSVGRVITRVINDVETLREFITWAVLAIARDLFALVA
                     IIVAMLALNVKLSLLTFVTIPFMILATVVYRRTARIAYRKVRAAVSWVNSVLAENING
                     IRVVQAFSRQSHNYDSFRNYTNRYYLQTAINAAKVAASFLPVVDVLGALATAAVVYVG
                     GTAVLGESITAGVLVAFVLYIDRYFEPIRDLSRRFDTLQSAMAGGERLLELLDTQVEV
                     RDADNAHAMEPIHGGVRFERVSFHYSDDPTLVLDGIELDVKAGETVALVGETGAGKTT
                     IVKLLARFHDPTDGCVLVDGVDLRKVTQRSLREQMGMVLQDPFLFNGSVRENILFGRL
                     NASDEEVIAAAQAVDAHDFIVGLKNGYDTSVEEGGIMLSVGQRQLISFARALLADPRI
                     LILDEATSSVDTQTEQVIQKALARLLKGRTSFVIAHRLSTITSADKIVVIEGGRIVEQ
                     GTHAELLEKRGKYFELYQTGFQE"
     assembly_gap    2474..2573
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2596..4392)
                     /locus_tag="PLE39_06285"
     CDS             complement(2596..4392)
                     /locus_tag="PLE39_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE67969.1"
                     /translation="MNILRLRSFVTAYLPQIFFSALNLGALTALNLYVPRIIRDVIDD
                     GLVGGDTGFLLRSALILLGLGLTAAALTALQRYLSEWIGSRVGYDIRNKLYNHIQNLS
                     FSYHDHAQTGQLISRCIEDVRAVQNFIGSSVVELTQLVFVLFGALFIMFSANWQLTLI
                     AVIPIFPLVWMTFKFGDRVTHLFFNVDNLLGELSARLQENVTGVQVVRAFAREPHEVK
                     RFDIVNRAYYNARLKVIGEWAKVMPSTDFLVTLCTILILWFGGQMVLNGTMTVGEIVA
                     FNAYVMMLAAPAQQLTWLVNAAGEADAGAKRVLEVLDAQPEIASPAAAVQLPSLRGEV
                     EFKNVSLKYLDEKRSSLTDVHLSVKPNQLVALIGQTGSGKTSLINLIPRFYDVSDGAV
                     LVDGEDVRSVDLVSLRRQIGIVLQTSLLFSDTIRENIAYGRPDATEEEVIAAAKAAQA
                     HEFITGFPEGYKTIVGERGVTLSGGQRQRVAIARALLMNPRILILDDSLSSVDTQTEK
                     LIQEALDNLMEGRTTFVIAHRLSTVRRADLILVMDKGHIVEQGTHTELLKRGGLYKEI
                     HDLQLAQNADPAEVTLFEARPEKAVYHSRDLN"
     gene            4515..5390
                     /gene="panB"
                     /locus_tag="PLE39_06290"
     CDS             4515..5390
                     /gene="panB"
                     /locus_tag="PLE39_06290"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNE67970.1"
                     /translation="MTETRKKITLPYLFKKVEDKQPITWLTCYDYPTAYLQEQAGIEM
                     ILVGDSLGMTMLGYDSTLPVKMDDMIRHAQAVRRGAPTAFVVGDMPYMTYQDSVENAI
                     RNAGRFMSEAGCDAIKLEGGAAMANRIKGIVDAGIPAIGHLGLTPQSVSALGGFRLQG
                     KSATLAKKILDDAKALEEAGAFCILLELVPDRLCKLITERAENCIIMSLGSGPDAHGQ
                     LLIYHDMFGLYPKFKPKMAKVYGNAGEVILNGLKGYHDEVIGGQFPEKDNYFGMPDEE
                     FEELKKMVEGGQLHR"
     gene            5450..6712
                     /locus_tag="PLE39_06295"
     CDS             5450..6712
                     /locus_tag="PLE39_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67971.1"
                     /translation="MANVQKLLKISPDKLKAFNSILLDPNSQIMKDFLAVVAKYGTPE
                     EINKKHRAARKMENLFKIVQERNPEAIKDLNWLIQQRDKGAFISVADYRKKVLGNAAK
                     KMKFKDKYAVTLEVSALQYFPWVRVMAEEAIANQTLMPGRYIAVRKMKESEADGDLPA
                     IVAALDIIGASFVETLDTKGTDGSNPHLGGPATITGYFGGIGQPNEHALMWLDEFLYY
                     YTNYGVQHVLNFNAGTILLAFLLYRLGVNIQFKISVFFGADNPYNALWILTMAKLLSR
                     DDGTSPLIGFNWSNSVNNQTMELTADFRKALDLEKVVRFEHHITEPQKSIVLQPYNRR
                     AELVELADHVANIAAKHEGGDPEVDVTRAHQSDILDYFREKKEVLASGDWDNLQRNYL
                     DKVDACNRTAWALTEKGLSFIAAKNLHK"
     gene            complement(7036..8523)
                     /locus_tag="PLE39_06300"
     CDS             complement(7036..8523)
                     /locus_tag="PLE39_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNE67972.1"
                     /translation="MKRLEQLTFTRFIAIIVVLFFHGGGGVYLNAIDFFPLSPILVSA
                     TTSVTYLYVLSGFVMSLVNHRPGEKFNIGKYWTARFLRLYPLYLLSFLLVCYFYADSV
                     AQIKLQKTLANVLILQAWIPRYAQSFNFAAWSMTVEFFFYAVFPFFTMWAYRQPMKKL
                     IWGSVILWTVSQTIHNALWVGYFPEYENFLVYFPVFHFSSFILGAVGGIWYLREGRNH
                     DYKPSMTLPVFIGGIFLACAYIVVSSRVPGLPHGLQMMAGLLAPILTVVIVALALDKS
                     RLSVFFNTPALVTLGDSSYGLYILHVPFFWLYERMLNNLGIADPKRLFDLTFLPLTIV
                     LVLLIHFYFDQPIRTWLKRILQNVSMPLLALDLCALGLSIYLAFTLRVDMGRESDLYR
                     PIALAMFWSAFALRTVFTVAFQAANPAMLRGSLFEFIRPVLISVTVGTVALGAIVFAG
                     YWAGAFHNFPRSILITDWAIMLALSLLVRYLFRQAGVYAPRPMSV"
     gene            complement(8615..8845)
                     /locus_tag="PLE39_06305"
     CDS             complement(8615..8845)
                     /locus_tag="PLE39_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNE67973.1"
                     /translation="MKAYIMIKIRAGDVKDVVLQLRKVGDVKEAHMTFGPYDAVAVVE
                     TSDLARLGSITALEIQPIPGVEQTLTCLAVDL"
     gene            complement(8861..9748)
                     /locus_tag="PLE39_06310"
     CDS             complement(8861..9748)
                     /locus_tag="PLE39_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite family transporter"
                     /protein_id="HNE67974.1"
                     /translation="MSEIRIDALLPAEMATRAEYLGVRKAEMPAFTMFMLSLLAGAFI
                     SLGAIFATTVASGNMAVTAADGAVAYTTGLPFGVTRLLTGLVFCLGLILVVVGGAELF
                     TGNNLIVMAWASGKVTGRALLRNWAIVYAGNFIASIATAALMFFTKQYTFGSNAVGVA
                     ALRIAVAKCDLGFVQAVALGILCNALVCLAVWLTYSARTTIDKILAIVFPITAFVAAG
                     FEHSIANMYFIPIALFIKDFDPQFVAAVGDKVPNLELLTWQAFFIKNLIPVTIGNVIG
                     GAVLVAAVYWVVFLRDKKA"
     gene            complement(9852..10412)
                     /locus_tag="PLE39_06315"
     CDS             complement(9852..10412)
                     /locus_tag="PLE39_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE67975.1"
                     /translation="MRRFFTLILIPFLFLTLSLIPFQWVDATSVQPAQVSTVVVTMIS
                     GTPIPVIHTSTPMEDGAVYHVVQADEALWSIALAYNTSVEQLKLLNSLSNNAIFIGQT
                     LLIVRPEVSTETPTPTLTVTLGIPTSTVTRPVTATATVTPTPLPVAPISRRSSGMVLG
                     AIVIIALLGAGIGAWLGSKRKAVAAE"
     gene            10557..11978
                     /locus_tag="PLE39_06320"
     CDS             10557..11978
                     /locus_tag="PLE39_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNE67976.1"
                     /translation="MPFKRIPFKKRVLAKIELAIKGPMFGCRMCGNCLLQETAFICPM
                     ECPKGLRNGPCGGSTPEKCYVDESRPCIWYKIYERSFKLGREALLLEVLPPMDWDKAG
                     TDAWSDVFEQAKQVGVKKIITGLTSGETGSVSKTLDSVFRPVRQPEWWQGDSLYHAPK
                     YTEPVSELERRLKAGEFAVAAEVAPPLSANTDKLKQNIEMLKPYVAALNFTDSPSATP
                     RMSSWACSKIAVDHGADPIMQIAARDRTRVGLQGEVMGAEALGVRNILCLTGDSMALA
                     PSPRGRMDIVDLDGIQILWILRRMRDEGKYLDGRELKTPPKYFLGAAASPFASNMKFQ
                     ALREQKKVNAGAQFFQTNVVFDADALDEWLNEIAKRDILDKVYIMIGLSPLKSYRAAV
                     FMNDKVPGVFVPEKILKRMENAKAKGNEQEEGVQITLELIEKIKGKQGVSGIHLMAVG
                     WEEIVPRIVREGLGVAPATATIK"
     gene            complement(12025..12438)
                     /locus_tag="PLE39_06325"
     CDS             complement(12025..12438)
                     /locus_tag="PLE39_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNE67977.1"
                     /translation="MTHIITSLCIRDRGCIEVCPVECIVPGQPVAEWPWMYIDPDTCI
                     DCGACVPECPYAAIFPEDEVPSAYSAKGGEYISKVGLTGHYEGTNHHGKAVVLETTRK
                     LDAGETVDLTPDIQPNYEFFKSGPGYSAKDSDQGS"
     gene            complement(12612..13217)
                     /locus_tag="PLE39_06330"
     CDS             complement(12612..13217)
                     /locus_tag="PLE39_06330"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNE67978.1"
                     /translation="MAFELPKLAYAYDALEPHIDARTMEIHHTKHHQTYITNLNGAVE
                     KTPELAGKSLEAMLSDLNAVPEGVRMVVRNHGGGTYNHNLFWEVMGPNAGGAPKGDLA
                     KAIDASFNSFDAFKDEFTKAATTRFGSGWAWLVKKGSGLAVVSTANQDTPLSDGLTPI
                     MGIDVWEHAYYLNYQNRRPDYITAWWNVVNWEAVSAKYAAK"
     gene            13345..14298
                     /locus_tag="PLE39_06335"
     CDS             13345..14298
                     /locus_tag="PLE39_06335"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE67979.1"
                     /translation="MQDSRARLLLPVIIPSAILAVSTASIFIRFTQAEGAPSLVIAAV
                     RLTIATLLLAPFALTNYLDEIKRFTRTEILLGAFSGIFLAVHFATWISSLEYTSVASS
                     AVFVSTGPLWVALLAPLLLKERLTKTAILGLILALVGGTVIGLSDACVWESGLICTDI
                     QNILQGRAMWGNILALIGALAVSGYLIIGRRLRNAGNMSLIPYIFLVYGMAAVTLNVI
                     MFASGHSPFGYSPKIYGLIFLLAAIPQLIGHSTFNWLLKFLPATFVAVTTLSEPIGSA
                     ILAYFILNETPTSFVIIGGCILLIGIYLTSREENNTSKESK"
     gene            14295..15560
                     /locus_tag="PLE39_06340"
     CDS             14295..15560
                     /locus_tag="PLE39_06340"
                     /inference="COORDINATES: protein motif:HMM:NF016310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HNE67980.1"
                     /translation="MTLQLSAKDQEMLKGQHGPATRMAMSILVRMAEVSGAKELLDIS
                     GAHIDSTVYIGDAGLEYAERLASLGAKVAVPTTLNVSGVDEHHWKEWAVPTLWAKQAH
                     RQMVAYQSMGTIPTWTCAPYQTEMRPRFGQQIAWGESNAIVFANSVIGARTERYPDMF
                     DVCCAITGRAPAIGLHLTENRAGKILLKLEGVSAELQASDDFYPVLGHYLGKISLDRI
                     PVIEGMTIVPDDDQLKALGAAAASSGAVALFHMVGVTPEAPTVEAAFQGNKPEETLIV
                     NMDTLRQARRELTHADDASLDMVVLGSPHFSLAEYKILAKLVEGKKKHADVKFLVTSS
                     RAMTQLAQQAGFLDPILQFGAQITVDTCILTTPMLPREIRFLMTNSAKFAYYAPGLLD
                     KRIAFSSLADCVTSAVKGEIVRDETLWQS"
     gene            15548..15967
                     /locus_tag="PLE39_06345"
     CDS             15548..15967
                     /locus_tag="PLE39_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HNE67981.1"
                     /translation="MAKLELFGKPVISGKANGIALVSREPLSFWGGYDWKSGEITDRR
                     HPLSGENASGKVLVVPFTRGSSTTTAVLLEAIREGTAPAAIITTATDFFFALASVVAG
                     ELYEKSFPLIALSEADFGAIKTGDQVKVMEDGKLIVE"
     gene            16016..16336
                     /locus_tag="PLE39_06350"
     CDS             16016..16336
                     /locus_tag="PLE39_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67982.1"
                     /translation="MSWLIVILIAVVVNIIVLATCGGGGGLMLLVALNGFSESAAMPF
                     LAGYGLLVLAISFGIATGATWIYVKPRSTASGIGFWQVAGLNAGIIVLLILGVVAFMA
                     IRAL"
     gene            16344..16826
                     /locus_tag="PLE39_06355"
     CDS             16344..16826
                     /locus_tag="PLE39_06355"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HNE67983.1"
                     /translation="MIIVVMGVAGSGKTTVGALLALELGWDFYDGDDFHSDANRAKMS
                     QRIPLNDEDRSTWLAALRDLINQNLSENRSIVLACSALKESYRHMLKVNEQVHFVHLQ
                     GSYQQIEERLRGRTGHFMSAEMLASQFDILETPEDALSINISNTPQEIVAIICKGLNP
                     "
     gene            16823..17320
                     /locus_tag="PLE39_06360"
     CDS             16823..17320
                     /locus_tag="PLE39_06360"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNE67984.1"
                     /translation="MKLPSHLYLDEKNIPYEALSFSPETEKGAANVAHALGFSERQAV
                     KTLIFQVDTGERVLVMLGGDQNAVSGNLKKAIGSRNIQMAAPDVVKETTGYVIGSIPA
                     FHWQPENFRSFLEASLLNEPILGTGGGQWGEEIMITPENLVKASNAIVVNLTDREKPV
                     FLESV"
     gene            17367..18629
                     /locus_tag="PLE39_06365"
     CDS             17367..18629
                     /locus_tag="PLE39_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67985.1"
                     /translation="MIGKSFPAKKTIVLVILSVAGGLLAVVNFFLLSKDMLRGDYSAK
                     DIFPQPMYGFFRVPDRPNTIAQNGVNRLAGDYAQVYFPSLEFSSLTQNYETGYLDPWK
                     RPSRYAPFIHYVCALSFCKLDYGYASLLHMLTQMVLFYAFFIAAFKILEIESDLWVGL
                     LITTLLLFVTPAGLSWFERGQFSLYVALAYLLLMLGLIKNKPVFVFISALFAYVKWTS
                     FPFLFVVLFVHWMSAKNTKERAQYTQSALIYLLVILVLSLAFRSRFIHFLEGIYKQEV
                     NVEAVGIALAQLLPTSLVKGMPILLILLGYLSLRKNNMDFERAAPFLIGSGILMLTYP
                     TVAFEYNIPNLFCFIPLIFYWARNPKLMNHAAKYVFFIFISLASCTAFLGSTVGAKTI
                     LFGYMAVAGFFLILPFIDNKTFGNALKG"
     gene            18637..19158
                     /locus_tag="PLE39_06370"
     CDS             18637..19158
                     /locus_tag="PLE39_06370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE67986.1"
                     /translation="MANPQIRIQTELGDMIVELYPAHAPITVENFLRYVDENRFEGAT
                     FYRVVHLENQPNNQVKIEVIQGGLKDEDHPQSLPNITHETTTQTGILHRDGVISMARN
                     EPGTASADFFICTGDQPELDFGGRRNPDGQGFAAFGKVIAGMETARRIQALPEENQYL
                     KTEIAFKIQRAQP"
     gene            complement(19236..20741)
                     /gene="malQ"
                     /locus_tag="PLE39_06375"
     CDS             complement(19236..20741)
                     /gene="malQ"
                     /locus_tag="PLE39_06375"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNE67987.1"
                     /translation="MTFKRSSGILLHPSSLPSPYGIGDLGPQSFRFIDWLASTGCKLW
                     QVLPLGPTGYGDSPYQAFSAFAGNPYLISPDELFADGLLTQEDLDGMKDLPASHVDFG
                     LFIPRKLDLLQKAFSRYQSNPEHLRQAFDYFCAENASWLDDYTLFMALKEANGGGAWN
                     GWPEALRKRKKAALSKAQKELGESVMRFAFYQFIFFRQWNKLRAYANERGIQIIGDIP
                     IFVAYDSSDVWSHPDLFFLDREGNPTVVAGVPPDDFSATGQLWGNPLYKWDVHKKEGY
                     AWWLSRVRSSLQIFDILRFDHFRGFAGYYEIPADHTTAEFGRWVTGPGKDLFRAVDKF
                     LGDGLITHGTGLPIIAEDLGLVTPDVIELLAAFDLPGMKVLQFAFSGPENPFLPHNYI
                     PNSVAYTGTHDNDTARGWFEHAAPHEREFAQRYLSVDGNDFAWDLIRGVWKSVAVFAI
                     TPMQDLLNLGSEARMNFPSKLGGNWEWRMGEYDLREDIAGKLRDLNWLTYR"
     gene            complement(20738..22162)
                     /locus_tag="PLE39_06380"
     CDS             complement(20738..22162)
                     /locus_tag="PLE39_06380"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNE67988.1"
                     /translation="MPKTINVLFLAAEAEPFIKVGGLGDVAGSLPRALRALSNDEITL
                     DVRLVLPYHPAIKAENLRPIGIFSIPRGNSEVQVDAFETSLKGMPVYFINGDAIRANG
                     SVYSLDAKLDAEKYSFYSIAALELSRQINWFPDVVHGNDWHTALSLYYSLTKRWEEGA
                     RHVASVLTLHNLPFMGPDVSGILESYGLKLAQTDLPDWARVMPLPLGLWSSDAIVGVS
                     PTYAKEVLTEEYGCGLNEFLQSRRETLSGILNGLDTDSFNPADDPAIGVNFALETLEK
                     RAANKGLLQARLGLPQDPEIPLFAIVSRMDVQKGVDLAFNALNTMKRLNWQAVILGTG
                     DPKLEEAAMKLQKLFPEKVKVETRYDAGLARQIYAGSDMFLMPSRYEPCGLSQMIAMR
                     YGCVPIVRAAGGLNDTVRHGVTGFVFEKAHHLSLVAAVKNALKVFANREQWQSLQRSG
                     MAQDFSWENSARQYLNLYLSLIKS"
     gene            complement(22223..22294)
                     /locus_tag="PLE39_06385"
     tRNA            complement(22223..22294)
                     /locus_tag="PLE39_06385"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22260..22262),aa:His,seq:gtg)
     gene            complement(22326..22790)
                     /locus_tag="PLE39_06390"
     CDS             complement(22326..22790)
                     /locus_tag="PLE39_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE67989.1"
                     /translation="MTISLHPVSAENWRALISLKVREDQTHFVASNLYSIAEAQFGFE
                     DEGHWEMFPFGAYAGEEPVGFLMYCLNFTHSRFQAFIARLMVDEKFQGKGYGRDIMSK
                     ALDRLRADERIRNVGISYEPENESARKLYASLGFEEPGEMYDGETLAVLNLR"
     gene            complement(22795..24066)
                     /gene="hisS"
                     /locus_tag="PLE39_06395"
     CDS             complement(22795..24066)
                     /gene="hisS"
                     /locus_tag="PLE39_06395"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNE67990.1"
                     /translation="MASKIQPVKGTREFYPEQMALRNFIYEKVRMASQKFGYQEWDAP
                     FIETIDLYAAKSGEELVKKQSFTFTDRGGDFVTLRPELTPSLARMIAAKQGELTFPVR
                     WWSFGPCWRYEQPQKGRTREFFQWNIDMLGVNSPEADAELIAVAATFLRSVGLTPERA
                     AIYVNNRRLMDSEFDALGLAPERRLDVSNLVDRRTKMDPAKWDSYALELGLTQTQLDG
                     LKGILGNFDLWKNSEELVRLFSALKALGVSEYVKFDPNIMRGLLYYTGTVFEAFETSG
                     SLKRAILGGGRYDNLLSDVGGQPLSGVGFAMGDVVAGIVLQEAGLVPEFIPTPAAVLV
                     TVFDEAMWLKSFELAAQLREAGINAMVFPEPAKLQKQFKFADKMKIKLAVTVGPDEAA
                     NGQAAVKNLMTGEQVTVQREAVVSEIRKMIG"
     gene            24229..25029
                     /locus_tag="PLE39_06400"
     CDS             24229..25029
                     /locus_tag="PLE39_06400"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE67991.1"
                     /translation="MKIDYQEPSKDLLMRIDIHEKYGSANIDVWTNDLLKPQAGMKIL
                     DVGCGAGKLCFLFNEFTKGGAQITGGDFSNELLDKAREKNKTVGANIDFQFLDFNQRF
                     NFADNTFDLCTSAFAIYYASDLDFTFGEAHRVLNPGGRLFVSGPLPENKQMFYDIIKE
                     ATGAPIPPMPGSSRFKGDIFNTIDKIFAKTELHKFENHLTFPEVAPFIDYVRASLGED
                     RRLWTSMFNGKDEYEALIGKIEAVATRWFERDGKLVMTKVVGGILATK"
     gene            25091..25723
                     /locus_tag="PLE39_06405"
     CDS             25091..25723
                     /locus_tag="PLE39_06405"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNE67992.1"
                     /translation="MNFFGKRVLFLGAHPDDIELGCGALLHKIAKKTDVLCVTLSDNQ
                     KNPDLQKVKSEHFQSMQVLDVPEEKIVLGPFTTRVFPDARQEILEYFLKLRKDFNPDL
                     IFTHTRQDVHQDHNTMTDEALRAFRGITVLGFDVVRSSYGFFPSFLVEVSEENAAAKI
                     EALSKYETYRDRYYFNAELTRSIMVRHGALAERPLAEGFDILRIVGKFGE"
     gene            25806..26507
                     /locus_tag="PLE39_06410"
     CDS             25806..26507
                     /locus_tag="PLE39_06410"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE67993.1"
                     /translation="MQKYERAVFISYAWGDEREEIVNQLDKALQKRGVKIIRDKRELE
                     YKGSISEFMERIGRGDCVIVVISDKYLRSPNCMFELIEIAENKKFRERVFPIILSDAN
                     IYDPVKRVEYVDFWENKRDELAKAIQKLDPANLQGIREDMDLYDRIRDMISGLASTLK
                     DMNALSPQTHRDADFEDLYNAIEQRLGQPQSARPKKPSKPAKIEKDETGTRNIEIGGS
                     ASGNVFIGDNNKIEK"
     gene            26541..28805
                     /locus_tag="PLE39_06415"
     CDS             26541..28805
                     /locus_tag="PLE39_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67994.1"
                     /translation="MANVSIGGNVTDSVLVFGNNNYVVKIGDVNGGVVNIVKPSDKPK
                     VSARPAPVMIKPRAFPSLMDRVDEAETVKKAAELAVPVSVWGKQGIGKTSFIRHLAHI
                     LPTNKYPNGVVYLNVSSLGHEDLLQALFDAFFESDNTYKPTTADILHGLQKISALIFL
                     DDLQIGRDETISILNAAPSSTFILISSERSFWGEGEAVQLQGLPVGDSVRLFEKELAR
                     PLETEEKITVPKICKLLQGHPLSILQSASLARESGKPLEEILNGLTNEKKDDKSMAYM
                     GLASLTDSEKQVIALLAAAGGTIVLAEHIKGIFKGDSRQTDIQKLIQLGFVQAHSPKF
                     SVTEAFASSLAATWDLSSWHDILLKYSIKWLSQQPASALVDESTNLLIHTVKTAGERK
                     KWSEVIQLGRALEKFLVYYKRWQAWADILKLIIEAAKALSDSKTQAWALHQLGTRALY
                     LGQTSEANSLLAQAQTIRKAIGDKAGLRVTQHNLKTIKGGSGCAKFVSYFVGAVVGAT
                     VLTGIAWLAINVLFSPPIPIALPTATNTPTLRPSSTIPPSNTPRPSETPTVRPSRTPR
                     PTDTPMPVLLYDFVESAHEVYWYQTTSNSDGEFTQDLPFFFEPVVPDVESYLPGSDSA
                     YVGWSFDTQLTNGNRYEKVLLTYPLYEHFKVFGDYTVKIGRPAPEAYLELTAGFKDIV
                     IEPVEGVMFRVYVNGKVYIEEPYYFGSEPIQTGSRIRLAANTYEFRLEVESLDPSPYD
                     FATWAVVRLWDKEP"
     gene            complement(28934..29281)
                     /locus_tag="PLE39_06420"
     CDS             complement(28934..29281)
                     /locus_tag="PLE39_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HNE67995.1"
                     /translation="MFNYTALDESKVEFLEIAPESELPNGERLFVDLGDKPIVIFNIA
                     GQFFAIGDICTHDDGPLGDGVIEDHNIVCPRHGAEFDVRTGKVMSMPAVVDIPAYPVQ
                     VRDGIIFIGVPKE"
     assembly_gap    29296..29395
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(29401..29796)
                     /locus_tag="PLE39_06425"
     CDS             complement(29401..29796)
                     /locus_tag="PLE39_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNE67996.1"
                     /translation="MDDLYREVIIEHYKHPSYRGKLDPHDISFADNNPLCGDHIQIDL
                     RVDAGGLVTDARFDGHGCAISQASADLLMETIIGKPLEEVKKLNKDSVLEMLGIDLGP
                     VRLKCALLSLKVLKAGVYGLGEASDSLVE"
     gene            complement(29829..31055)
                     /locus_tag="PLE39_06430"
     CDS             complement(29829..31055)
                     /locus_tag="PLE39_06430"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNE67997.1"
                     /translation="MKLNVSQVRKDFPILEREIRAGVGLTYLDSTATSQKPLAVIEAM
                     NEYYRLSNANIHRGVHALAEEATAMYEGARERIAKFINAASAREVIYTRNTTESINLV
                     AYSWARANLKQGDLVILTEMEHHSNLVPWHMLQAERGIELEFIPVTEDGVLDLEVYKT
                     LLDRRPKLVSFTHMSNVLGTINPAAEMIRLAHAVGAITLVDAAQSVPHLTVDVQALDA
                     DFLAFSAHKMCGPTGIGALYGKTALLEAMPPFLGGGDMIKEVKLRSFRANTLPHKFEA
                     GTPAIAEAIGFGAAVDYLSKVGMDAIAAHEHEITEYALERLEEIPGVKLFGPSADKKG
                     GVAAFTLEGVHPHDVAQILDQDGIAVRAGHHCAQPLHEKFGIPATSRASFYLYSTKEE
                     VDLLVNGIYKVKEMFG"
     gene            complement(31095..31697)
                     /locus_tag="PLE39_06435"
     CDS             complement(31095..31697)
                     /locus_tag="PLE39_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE67998.1"
                     /translation="MAVKSNSKRMVKPSAKAKTARTVSKKRVAVKTKTTVKTKVIKTE
                     VKKKPMQASKVVTAAPKKEVEQQTSNHPTAVKPAVTPVIKRVVNQMVERRRPVARPSR
                     TNQFSFMKAPGAEHDYEVGGSVEVFCDHEKNRERIRGWVKGIVVQVDNKMVAVQFRSN
                     VYLTDGWMVPDRILWFPLNSEHMRPVPGKKPPVKDFIPEY"
     gene            complement(31846..33183)
                     /gene="sufD"
                     /locus_tag="PLE39_06440"
     CDS             complement(31846..33183)
                     /gene="sufD"
                     /locus_tag="PLE39_06440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNE67999.1"
                     /translation="MQEKPKVTVTRQRRADTAAKEFAFTQADVSAAGSLASFRASAWE
                     AFNKHSLPVNTDEAWRRTDLRLLPASDFKLPKIGAFDDLPAVPEYLLQPLTGEQHGGQ
                     ITLLPGGSKVDLDESLVKKGVVFTDFRTAEKNHADLLAKLIGQIVKPDDGKFAALASA
                     LAQNGVLLYVPRNVQVEQPLHSVLWGPGSELAHFSHLLVYVEDGASVTYVHEAASPDE
                     VGHAMHAGVVEIYVGKDANLRFVELQSWGRHVWNFSHERARVERGGNLDWIFGAVGSR
                     LTKNFSDLDLAGEGSVGRMSGFYFTDGTQHLDHDTQQNHLAPHTTSDLLFKGALKGKS
                     RSVWQGMIYVAPGAQKTDGYQANRNLVLDDKARADSIPGLEILADDVRCTHGATVGKL
                     EQEPLFYLKSRGIPQREAERLVVEGFFDPIMQRIPFEGVRERFQQAIQDKLSA"
     gene            complement(33251..34660)
                     /gene="sufB"
                     /locus_tag="PLE39_06445"
     CDS             complement(33251..34660)
                     /gene="sufB"
                     /locus_tag="PLE39_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNE68000.1"
                     /translation="MSEDTKILDEIGEYKYGFHDRDDNYTFKSEKGLSREVVENISRM
                     KGEPKWMLEFRLKALEHFLARPMPNWGPELSSLDLDNIYYYVKPTEKSEKSWDDVPDD
                     IKRTFDKLGVPEAEQKFLAGLGAQYESEMVYHSIQEHLEKQGVIFLSIEDGLRQHPEL
                     FREYFGTVVPIEDNKFAALNSAVWSGGSFVYVPKGVKVDLPLQAYFRLNTANVGQFER
                     TLIIVDEGAQVHYVEGCTAPQYTTDSFHSGVIEIIVKKGARSRYSTIQNWSTNVYNLV
                     TQRAKVFENGTHEWVDANLGSKVTMKYPSCYLMEPGAHGEMLSMAFAGTGQIQDAGSK
                     MVHFAPNTTSKITSKSISKNGGRASYRGLLKVYKGAKGVKSNVVCDALLLDPKSRSDT
                     YPYIEIDEDDVTIGHEASVSKVGEEQLFYLMSRGLSEEEATTMVVSGFIEPLVKELPM
                     EYAVEMNRLIQLQMEGSIG"
     gene            complement(34734..35510)
                     /gene="sufC"
                     /locus_tag="PLE39_06450"
     CDS             complement(34734..35510)
                     /gene="sufC"
                     /locus_tag="PLE39_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNE68001.1"
                     /translation="MAQLEIKDLHVSIEDKEILKGLSLTIKQGETHAIMGPNGTGKST
                     LAYTLMGHPNYTVTSGEVLLDGENILEMEPDERSRAGVFLAFQYPVAIPGVTVANFLR
                     SAINSRRRAENPEDKGMPIPEFRKMLKEKMDMLKMDHNFAGRYLNEGFSGGEKKRAEI
                     LQMATLKPRFAVLDETDSGLDIDALRIVSEGVNALSGPELGVLVITHYQRLLNYIKPH
                     FVHVMMNGRIVESGGPDLALHLEDQGYDWVREKYDAVAEG"
BASE COUNT         9294 a         9222 c         8705 g         8256 t
ORIGIN      
        1 acagcggaat cgtaaagaag aacgaactcc ccgaacccag gtcactctca aaccaggtct
       61 cgccgtcgtg caggtcaaca atgtgctcca cgatcttcaa ccccagccca aagccgaggc
      121 gcggatcgtc cttgtcttcc aaaatggtct cgaaaggctg aaagaccagt tcttgattct
      181 cctcggcgat gcccatgccc gtatcgcgca cctcaaagag aatgtcttcg ggctcgcgtg
      241 aaaccaacac cgtgaccgtt ccgctttcgg tgaatttcac cgcattgtgg atcaaattca
      301 acaacgcctg cgaaagccgt gtggcgtctg cctcgatcag aggcaggtca tcttcgatct
      361 catcttccag ggtcactccg ctcttttgct ccaccaactg gtcggcaatg gtcaacaccc
      421 catcgagcag cgcatggata tccacctctt ccaggttgag cgccatcctg cctgtcacgg
      481 tctcggcgta atcttgaaaa tcactcacct gcgcgatcat cggagccagc gccgcctgaa
      541 tgtactccat gggcgaatcc ttcacgccga tgtaatcctc caactcctcc aaacggtaca
      601 ggtcattgac gttcttctgc acatcagcca gccccctgcg cagcgagttg gacattcgat
      661 acaaaaattc attctgggtc gcattggtat tggtcatggg gttctactcc tgaaagccag
      721 tttgatatag ttcaaaatat ttgccgcgtt tttcgagcag ttcagcatga gtgccctgct
      781 ccacgatcct gccgccttcg ataacgacga ttttatccgc actggtaatg gtagacaagc
      841 gatgcgcaat gacgaaggag gttcgtccct tcaacaaacg cgccagcgcc ttttgaatca
      901 cctgctcggt ctgtgtatcg actgaagaag tggcctcatc gaggatcaag atccgcgggt
      961 cggccagcag cgcacgcgcg aacgagatca actgtctctg ccccacgctg agcataatgc
     1021 cgccctcctc caccgaagta tcgtatccgt tcttcaaacc cacgatgaaa tcatgcgcat
     1081 ccacagcttg cgcggcagcg atcacttctt cgtcactggc attcaaacgt ccaaacaaaa
     1141 tattctcgcg cacactgcca ttgaacagga aggggtcctg caacaccatg cccatttgct
     1201 cacgcagcga acgctgagtc actttccgca agtccacacc gtctacgagc acgcagccat
     1261 cggttgggtc atggaagcgg gcgagcagct tcacaatcgt cgtcttgcct gcgcccgtct
     1321 cccccaccaa cgccaccgtc tcacctgcct tcacatcaag ctcgatccca tccaaaacaa
     1381 gggttggatc atcggaatag tggaaggaaa cccgctcgaa tcggactccg ccgtgaatgg
     1441 gttccatcgc atgagcattg tctgcatccc gaacctccac ctgcgtatca agcaattcca
     1501 gcagtctctc gccgccagcc atggccgatt gcagggtgtc aaaacggcgg ctcaaatcac
     1561 ggatcggctc gaagtaacgg tcaatgtaca aaacaaaagc caccaacacc cccgccgtga
     1621 tcgattcacc caacactgcc gtgccgccca catataccac cgccgcggtt gccagtgcgc
     1681 caagcacatc caccacaggc aaaaaggacg cggccacttt ggcggcattg atcgcggtct
     1741 gtaaataata gcggttggtg tagttgcgaa acgaatcgta attatgcgac tgacggctga
     1801 atgcctgcac cacacggatg ccgttgatat tttccgccag caccgagttc acccatgaaa
     1861 ccgccgcgcg caccttgcga taggcaatcc gcgcggtgcg gcgatagacc accgtggcaa
     1921 ggatcatgaa cgggatggtc acgaaagtaa gcagggatag ttttacattg agcgccagca
     1981 tcgccacgat gatcgccacc agcgcaaaca ggtcacgcgc aatggccagc accgcccagg
     2041 tgatgaattc gcgcagggtc tccacatcgt tgatcacacg cgtaatcacg cgccccaccg
     2101 agtaacggct gaagaatccc agcgaaaggc tctgcaaatg ctcgaacaac aacttgcgca
     2161 tgtcgtagat caccgactgc cccacccgcg ccatgatatt cacacgccaa tagatgaagc
     2221 cccaacggat gatcgccgcc gcaaggtaaa acaacacgac gtttcgcagc acggtgtaat
     2281 tccctgccac aaggccgtcg tcgatcgcaa ttttgacaag gtacggtccc atcacagaag
     2341 aaagcacggc caccagcatc aggaaagccg aaaacagcaa ttgccattga tacggtgcta
     2401 caaatccgat cagccttttg gcaatattcg ggtcaagcgg tttgtcgtag aattcttccg
     2461 actgggtaac gtannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaagggta
     2581 aaggttgtgg gtggatcaat tgaggtctct ggaatgatag acggcttttt cagggcgcgc
     2641 ttcaaataac gtgacctcgg cagggtcggc attttgagcc agctgcagat cgtgaatttc
     2701 cttgtacagg ccgccgcgtt ttagcaattc ggtatgggtg ccctgttcaa caatatgtcc
     2761 cttgtccatc acaaggatca ggtcggcacg gcggacggtg gagaggcgat gcgcaatgac
     2821 aaaggtggtg cggccttcca tcaggttatc caacgcttcc tgaatcagtt tttcggtctg
     2881 cgtatccacg ctggaaaggg agtcatccag gatcaaaatg cgcggattca tcagcaaggc
     2941 acgggcgatt gccacccgct gacgctgccc gcctgaaagg gtcacaccgc gttcgcctac
     3001 aatggtcttg tagccctcag ggaaacctgt gatgaattca tgggcctgcg ccgctttcgc
     3061 cgccgcaatg acttcctctt cggtggcatc gggtcggccg taggcaatat tctcacggat
     3121 ggtatcggaa aaaagcaggg aggtttgcag cacaatgccg atctgcctgc gcagggaaac
     3181 caggtccacg ctgcgaacgt cctcgccatc cacgagcaca gcgccatcgc tcacatcgta
     3241 aaaccgcgga atgagattga tgagcgaagt ttttccgctg cccgtctggc cgatcaacgc
     3301 aaccagttgg ttgggcttca cactcagatg gacatcggta agggaggaac gtttttcatc
     3361 aaggtatttg agcgagacgt ttttgaattc cacttcacca cgcaggctgg gaagctgcac
     3421 cgccgccgca ggtgaggcga tctcgggctg cgcatccaac acttcaagca cgcgctttgc
     3481 acctgcatcc gcttcaccag cggcattcac cagccaggtc aactgctggg cgggggcggc
     3541 cagcatcatc acgtaggcat tgaatgcaac gatctcaccg acggtcatcg tgccattcaa
     3601 gaccatctgt ccgccgaacc acaaaatgag aatggtacac agcgtgacaa ggaaatcggt
     3661 ggaaggcatc accttcgccc attcgccaat caccttgagg cgggcattgt aataggcgcg
     3721 gttgacaata tcaaaacgct tgacctcatg cggctcacgg gcaaaggcgc gtacaacctg
     3781 cacacctgtc acgttttcct gcaggcgtgc agaaagttcc ccaagcaggt tatccacatt
     3841 gaaaaagaga tgggtcacac ggtcgccgaa cttgaaggtc atccacacca gcgggaagat
     3901 tggaatgacc gcgatcaagg tcaactgcca attggcgctg aacatgatga acagcgcccc
     3961 aaacaggacg aagaccaact gggtcaactc caccacagaa cttccgatga agttctgcac
     4021 ggcacgcaca tcttcgatac agcgggagat gagctgccct gtctgggcat ggtcgtggta
     4081 tgaaaacgaa agattctgga tgtggttgta caacttattt cgaatgtcat accccacacg
     4141 cgaaccgatc cattcggaaa gataacgctg aagggcggtc aatgccgcgg cggtcagacc
     4201 cagcccgagc aagatcagag ccgagcggag caggaatcca gtatcgccgc cgaccagtcc
     4261 gtcgtcgatc acatcacgga tgatgcgcgg cacatacagg ttcaaggcgg taagggcgcc
     4321 caaattgagc gcagaaaaga aaatctgcgg caaataggca gtaacaaagg agcggagtct
     4381 aagtatgttc atggtttatg cggggtgtta aggataccac gagaaaatca atcaaacatc
     4441 atgattttcg tcagttgtac ccgcctgcct cccgcttata tactgaaatc acacccatcc
     4501 aaaaaggagc aagaatgacc gaaacgcgca agaaaatcac cctgccgtat ttgttcaaaa
     4561 aggtcgaaga taaacagccg atcacctggc tgacctgtta cgactaccca actgcttatc
     4621 tgcaggaaca ggcgggaatc gaaatgattt tggtcggcga cagccttggc atgacgatgc
     4681 tcggctacga ctccaccctg cccgtcaaaa tggatgacat gatccgccat gcacaagccg
     4741 tgaggcgcgg cgcaccaacc gcatttgtgg tcggcgatat gccgtacatg acctatcagg
     4801 attctgtgga gaacgcgatc cgcaatgctg gccgattcat gtcagaagct ggatgcgatg
     4861 ccatcaagct cgaaggtgga gccgcaatgg cgaatcgtat caaagggatc gtggatgctg
     4921 gcatcccagc cattggccac cttgggctga ctccgcaatc tgtctccgct ctgggtgggt
     4981 tccgcttaca agggaaatct gccacgctgg caaaaaagat cctggacgat gcaaaagcgc
     5041 tcgaagaagc aggcgcgttc tgcattctgc ttgagttggt acctgaccgt ttgtgcaagc
     5101 tcatcaccga acgcgccgag aactgcatca tcatgtcgct tggctcaggg cctgatgcgc
     5161 atggtcaatt actgatctat cacgacatgt tcggcttgta tcccaaattc aaaccaaaaa
     5221 tggcaaaggt ttacggaaac gctggcgaag tcattctgaa cggactcaaa gggtatcacg
     5281 atgaggtgat tggcggacaa ttccccgaaa aagacaacta cttcggcatg cctgacgaag
     5341 aattcgaaga gttgaagaag atggtcgaag gcggccagct gcatcgataa caacacaggc
     5401 ggcagtcatt ctggtcataa actgccgcct gcttacacaa ggagcaagaa tggcaaacgt
     5461 acagaaactt ctcaagatct cgcccgataa actcaaagca ttcaactcga tccttcttga
     5521 ccccaattca cagatcatga aagatttcct tgccgtggtg gcaaagtacg gcacacctga
     5581 agagatcaat aaaaagcacc gcgccgcccg caaaatggaa aatctcttca agatcgtgca
     5641 ggaaagaaac cccgaggcca tcaaggatct gaattggctc attcaacaac gtgacaaagg
     5701 cgcctttatt tcagtagccg actatcggaa gaaagtgctc ggcaacgcgg caaagaagat
     5761 gaagttcaaa gacaaatatg ccgtcacgct ggaggtttct gcgctgcaat attttccatg
     5821 ggtccgcgtc atggcagagg aggcgatcgc gaatcaaacc ctcatgcctg gccgctacat
     5881 tgccgtccgc aagatgaagg aatctgaagc ggatggagac ctgcctgcca tcgtcgccgc
     5941 tctggatatc atcggcgcgt cctttgtcga aacactcgac accaaaggca ccgacgggtc
     6001 gaatccacac ctgggcgggc cagccaccat cacaggctac tttggcggca tcggccaacc
     6061 caatgaacac gcgctgatgt ggctggacga attcctgtac tattacacaa attacggtgt
     6121 tcaacatgtg ttgaatttca acgcaggcac gatcctgctg gcctttttac tgtatcgtct
     6181 gggggtgaac atccaattca agatatccgt cttcttcggc gcggataatc cctacaatgc
     6241 cctatggatt ctgaccatgg ctaaactgct cagccgtgac gatggaacca gtccactgat
     6301 cggattcaac tggtcaaact cggtcaacaa ccagaccatg gaattgaccg ccgatttccg
     6361 caaagcactg gatttggaaa aggtcgtccg ctttgagcat cacatcaccg agccacagaa
     6421 gagcattgtg ctacagccat acaatcgccg cgctgaattg gtggaactcg ccgatcacgt
     6481 cgccaatatc gccgcaaaac acgagggcgg cgaccccgaa gtggatgtca cccgcgcaca
     6541 tcagtctgac attctggatt acttccgcga gaagaaggaa gtccttgcct cgggtgattg
     6601 ggataatcta caacgaaact acctcgacaa ggtggatgcc tgcaatcgca ccgcctgggc
     6661 gttgacagaa aaagggctaa gtttcatcgc cgcaaagaat ttacacaagt aaacaaaaac
     6721 cgcaaccatg attcactcca aacttaggga atcagctgtg aattttgaat agacctcttg
     6781 aaaagagatt aaccactgag ccacggagac accgagaaaa caattaaaac tccgtccgca
     6841 ggaggctccg caccgcctca gtgtctccgc ggttcaaaat ggttttccaa aaacattcca
     6901 tgcaacttat gcaagatacg ggcaagttac aaaaaggccg accctgtaaa acgggtcggc
     6961 ctttttgtgt aaattcgcag ctaaagtatt gaaaaaacaa tttcgggatg tttcatattt
     7021 gcgagcccaa aacacttaca cagacatggg tctgggagca tataccccag cctgcctgaa
     7081 caaataccga accaacaggg aaagcgccag catgatcgcc caatcagtga tcaaaatact
     7141 gcgcggaaaa ttatgaaatg cgccagccca atagccagcg aaaacaattg cacccaaagc
     7201 aacagtccca actgtgacag agatcaaaac agggcggata aactcaaaca atgatccgcg
     7261 caacatggca gggttcgctg cttgaaaagc cacagtaaac accgtccgca gggcaaaagc
     7321 tgaccaaaac atcgccagtg cgatcggacg gtacagatca gattcgcggc ccatatccac
     7381 gcgcagggtg aaggcaaggt aaatggaaag tcccagcgcg caaagatcca gcgccaggag
     7441 aggcatgctc acattttgta gaatccgctt cagccaggtg cgaatcggct ggtcaaagta
     7501 aaagtggatc aacaaaacga ggacgatcgt caacggcagg aaggtcaggt cgaaaagccg
     7561 tttcgggtca gcgataccca ggttattcaa catgcgctca tacagccaga aaaatggaac
     7621 atgcaaaatg tataacccat aagaagaatc gccgagagtg accaacgcag gggtgttgaa
     7681 gaaaaccgaa agacgagatt tatccaacgc cagcgccaca atgacaacgg tcagaatcgg
     7741 ggcgagcagt ccagccatca tttgcagtcc atgcggcaaa cctggcaccc tgctgctgac
     7801 gacaatataa gcacatgcca gaaaaatgcc gccaataaaa acaggcaatg tcatgctggg
     7861 tttgtaatca tggtttcgcc cctcacgcag ataccagatc ccacccaccg cccccagaat
     7921 gaacgaacta aaatgaaaca cagggaaata aaccaggaaa ttttcgtact cgggaaagta
     7981 accgacccaa agcgcattat ggatggtctg gctgaccgtc cataaaatga cggagcccca
     8041 gatcaatttc ttcatgggtt gacggtacgc ccacatggtg aagaaaggga ataccgcata
     8101 aaagaaaaac tccacagtca tggaccatgc tgcgaaatta aaagactgtg cgtaacgggg
     8161 aatccacgcc tgcaggatca gaacatttgc aagcgttttt tgcaatttga tctgggcaac
     8221 cgaatcggca tagaagtaac aaaccaacag aaacgacaaa aggtacaacg gatataaacg
     8281 taaaaaacgc gcagtccagt atttaccaat attgaacttt tcacccgggc gatgattcac
     8341 aagcgacatg acaaagccag aaagcacgta caagtaagtg acactggtgg tggcggaaac
     8401 caggatcggt gaaagcggaa agaagtcaat ggcgttcagg tacacaccgc cgccaccgtg
     8461 gaagaacagc accacaatga tcgcaatgaa acgggtaaag gttaattgtt cgagtctttt
     8521 catccaaatc ctcaaggaac tgcgcctatt ttactggaaa aaatcgggct ggcatcagcc
     8581 agcccgatgg ggaaaacagg gggaacttcc aagtctacaa gtctacagcc agacaggtga
     8641 gggtttgttc cacacctgga atgggttgga tctccaaagc ggtgatcgag cccagcctgg
     8701 ccagatcaga ggtttcgaca actgccaccg cgtcatacgg gccgaaggtc atgtgcgcct
     8761 cttttacatc ccccaccttg cgaagctgca aaaccacatc tttcacatcg cccgcgcgga
     8821 tcttgatcat gatatacgct ttcatggttt ctccttttgt ttatgctttc ttatcacgaa
     8881 ggaacacgac ccaatagaca gcggccacca acaccgcacc gccgatgaca ttgccaatgg
     8941 taacagggat tagattttta atgaagaaag cctgccatgt aagaagttca agattgggaa
     9001 ccttatcgcc aaccgctgca acgaactgcg ggtcgaaatc tttgatgaaa agcgcgatgg
     9061 gaatgaaata catgtttgca atgctgtgct caaaacctgc cgcaacaaag gctgtgatcg
     9121 ggaaaacgat cgccaggatc ttgtcaatgg tggtgcgtgc gctgtaggtc agccacaccg
     9181 ccagacacac cagcgcattg cataaaatgc ccaaagcaac cgcttgcaca aagcccaggt
     9241 cacatttggc tacggcaatt cgcagcgcag ccacgccaac cgcattggaa ccaaaagtat
     9301 attgtttcgt gaaaaacatc aaggcggcag ttgcaatgga agcgatgaag ttccccgcat
     9361 aaacaatggc ccaatttcgc aataacgccc gtccagtcac cttgccactg gcccaagcca
     9421 tcacgatcaa attattccct gtgaataact ccgcgccacc tacaaccaca aggatcagac
     9481 caaggcagaa aaccaacccg gtcaacaggc gtgttactcc gaaaggcagc ccggtggtat
     9541 aggccactgc cccatccgct gcggtaacgg ccatgttccc agaagcaacg gtcgttgcaa
     9601 agatggcgcc cagtgaaata aacgcaccag ccagcaggga aagcatgaac atcgtaaaag
     9661 caggcatttc cgctttgcgt acaccaaggt actcggcgcg ggtggccatt tcggcgggta
     9721 gaagtgcatc aatccgtatc tcactcataa atactccttt tcaagaactg atgttaactt
     9781 ttccacactt tcactatatc aaagcctggt cttcaaaatc aaagtggttt tgtaatggaa
     9841 agcaggtgat gttactctgc tgctacggct ttccgcttgg aacccaacca tgcaccaatg
     9901 ccggcaccaa ggagcgcaat tataacgatc gctcccagaa ccattccgct tgaacgacgg
     9961 gagatcggcg ccactggcaa cggagttggt gtgaccgttg cggttgcagt tacgggccgt
    10021 gtcaccgtgg aagttggaat ccccaaagtg actgtaaggg ttggagtggg agtttctgtg
    10081 ctcacttcgg gtctcacgat caacagtgtc tgtccgatga aaatcgcatt gttgctcagg
    10141 ctgttgagca acttcaactg ctccaccgag gtgttatagg caagcgcaat gctccacaaa
    10201 gcttcatcag cctgcacaac atgatagacc gcgccgtctt ccatgggtgt gctggtgtgg
    10261 atcacgggaa tgggagtgcc cgatatcatg gtcacgacca ccgttgaaac ttgagcaggt
    10321 tgtaccgagg tggcatccac ccattgaaac ggaatcaggc tcagggttaa gaaaaggaac
    10381 ggaattaata tcaaagtaaa aaatcgtcgc attcaaaaat tataccgtcc ccggcacaca
    10441 tccaaggact tctcaaattc accagtgcgg gtgtaaaatt atatataata tataatccca
    10501 aggagaccgt tcccatgccc ggctttactc caggacgccg ctttcaaccg ccgtttatgc
    10561 ctttcaagcg aataccattc aagaaacgtg ttcttgcaaa gatcgaatta gcgatcaaag
    10621 gccccatgtt tggatgtcgc atgtgcggga attgtctctt gcaggaaacc gcatttatct
    10681 gccccatgga gtgcccaaag ggattgcgta acggaccgtg cggcggctcg actcctgaaa
    10741 aatgttatgt ggacgagtcg cgtccatgca tttggtataa gatctacgag cgttctttca
    10801 aactcggccg cgaagctttg ctgctcgaag tacttcctcc aatggattgg gataaggcgg
    10861 gaaccgatgc ctggagcgac gtatttgaac aagccaaaca ggtcggcgtg aaaaagatca
    10921 tcacgggtct tacttcgggt gaaactggtt ctgtttcgaa gacgcttgat tctgtctttc
    10981 gacccgtacg ccagcccgag tggtggcagg gcgattctct ttaccatgcc ccaaaatata
    11041 cagaacctgt ttcggagttg gagcgccgcc ttaaggccgg ggaatttgcc gtggccgccg
    11101 aagtcgcccc tcccctttca gcaaacacag acaagctcaa acaaaacatt gaaatgctca
    11161 agccttatgt tgctgcgctg aatttcaccg acagcccttc ggctactccg cgcatgtcat
    11221 cgtgggcatg cagcaaaatt gcagtagatc atggagccga cccgatcatg cagatcgcag
    11281 cccgtgaccg cacacgtgtg gggttgcagg gcgaggttat gggcgccgaa gcccttggcg
    11341 ttcgcaacat cctgtgtctg acaggcgaca gcatggcatt ggccccctct ccacgcgggc
    11401 gcatggatat tgttgatctc gacggcattc aaatcctatg gattctgcgg cgaatgcggg
    11461 atgaaggcaa gtatcttgac gggcgcgaac tcaaaacacc cccgaaatat tttctgggtg
    11521 cggcagcttc accctttgct tccaatatga agttccaggc attgcgcgaa cagaaaaaag
    11581 tgaacgcagg cgcacaattc tttcaaacca atgtcgtctt cgacgctgac gcacttgatg
    11641 aatggttgaa cgaaatcgcc aaacgcgata ttctcgacaa ggtttacatc atgatcgggc
    11701 tgtctccgct gaagagctac cgcgccgctg ttttcatgaa cgacaaggtg ccaggagtat
    11761 tcgtccctga gaaaattctc aaacgcatgg aaaatgcaaa ggccaaaggc aatgaacagg
    11821 aagaaggtgt gcaaatcaca ctggaactga tcgaaaagat caagggcaaa caaggcgtaa
    11881 gtggaattca cctgatggct gtgggatggg aggaaatcgt cccacggatt gttcgcgaag
    11941 ggttgggagt ggcacccgca acggcaacaa tcaaataaac aaaaaagcca ggcaagcgcc
    12001 tggctttttt gttgcgatga ttgattacga accctgatcg ctgtctttgg cggaataacc
    12061 ggggccactc ttgaagaact catagtttgg ctggatatcc ggggtcaggt cgacggtttc
    12121 gccagcatca agctttctgg tggtttcgag gacaacggcc ttgccgtggt ggttggtacc
    12181 ttcgtagtga ccggtgagac cgaccttgct gatgtattcg ccgcccttgg cagagtaagc
    12241 cgaaggtact tcatcttctg ggaagatggc ggcatacggg cactcgggaa cgcaagcgcc
    12301 gcagtcgatg caggtatcgg ggtcgatata catccacggc cactcagcaa cgggctggcc
    12361 gggaacgata cactccacgg ggcatacctc gatgcaaccg cggtcacgaa tgcacaggct
    12421 ggtaataata tgtgtcatgg taatttactc cttgtaggat tgtaattaaa ccgaacaaac
    12481 ctattataac ttcgtttatc tttgtgacaa gggacacagt ggaaagtaag acgccccctc
    12541 aaaagataag tttgaaaaaa acatcccgca gaaatctgcg ggatgttttg aaatggattg
    12601 tccttacgct gttattttgc agcgtatttg gcggaaaccg cttcccagtt gaccacattc
    12661 caccaggcgg tgatgtaatc ggggcggcga ttctgataat tgaggtagta ggcatgttcc
    12721 cacacatcaa tacccatgat cggggtcaga ccgtcagaaa gcggagtatc ttgattggca
    12781 gtggaaacaa cagcgaggcc gctgcccttt tttacgagcc aggcccagcc agaaccgaag
    12841 cgggttgttg cggctttggt aaattcatcc ttgaaagcgt caaatgaatt gaaagacgca
    12901 tcgatcgctt tggcaaggtc gcctttcgga gcgccgcctg cattgggtcc catcacttcc
    12961 cagaacaggt tgtgattgta ggtaccgccg ccatgattgc gcacaaccat gcgcacacct
    13021 tcaggaacgg cattcaggtc actgagcatc gcttcgaggg acttaccagc aagttcaggg
    13081 gtcttttcca ccgcgccatt caagttggtg atgtaggtct ggtggtgttt ggtgtgatgg
    13141 atttccatcg tgcgcgcatc aatgtggggt tcaagcgcat cgtacgcata tgcaagtttg
    13201 ggaagttcaa aagccatgtg ggttctcctt tgagttgata atttcgtttg aatgtataat
    13261 tcgattttac ccccttataa caatacccgc aagaaatttg acaattttta tacaaatatc
    13321 atataaatca accccagaga gtcaatgcaa gattcccgtg cgcggctgtt gttgccagtt
    13381 atcattccca gtgccattct cgcggtctca accgcgagta tttttattcg attcacccaa
    13441 gccgaagggg caccttcact ggtgatcgca gcagtcagat tgaccattgc gactctcctg
    13501 ctggcgcctt ttgcattgac aaactatctg gacgaaataa aacgcttcac acggaccgag
    13561 attctgctcg gtgctttctc gggcattttc ctcgccgtac attttgccac atggatctca
    13621 tctctggaat ataccagcgt ggcaagttcg gcggtcttcg tcagcacggg gccgctttgg
    13681 gtggcgctgc tggctccgct cttgctgaag gagcgtctga caaagacagc cattctcggg
    13741 ttgatccttg ccctcgtagg cggaacagtt atcgggttgt cagatgcgtg tgtctgggag
    13801 tcaggactca tctgtactga tatccaaaac atcctgcaag gccgtgctat gtggggaaat
    13861 atcctcgcat tgattggggc attggcggtc agtggatatc tcatcatcgg caggagattg
    13921 cgcaatgctg gcaacatgtc actgatccct tatatttttt tggtgtatgg catggcggca
    13981 gtgacattga acgtcattat gttcgcctct ggtcactccc cttttgggta ttcccccaag
    14041 atatacggat tgatttttct gctggcggct attccgcaac taattgggca ctccacattc
    14101 aattggcttt tgaaattcct gcctgctact tttgtggcgg tgaccacttt gtcggaaccc
    14161 atcggctcgg caatcctcgc ttattttatt ctgaatgaaa ccccgacttc atttgttatc
    14221 ataggcgggt gcattctctt gatcggtatt tatctcactt ccagagaaga aaacaacaca
    14281 tccaaggaat caaaatgacc ctccagttat cagcaaaaga ccaggaaatg ttgaaaggcc
    14341 agcacggccc agccacccgc atggccatgt ccattttggt acgcatggca gaagtttcag
    14401 gtgcaaagga attactcgat atcagcggcg cacatattga cagcactgtt tacatcggcg
    14461 atgcagggct tgaatatgca gaacgcctcg ccagccttgg tgcaaaggtg gccgtaccaa
    14521 ccacgctcaa cgtgagcggt gtggatgagc accactggaa ggaatgggct gtacctacgc
    14581 tgtgggccaa acaggcacat cgtcaaatgg tggcatatca aagcatgggg accatcccaa
    14641 catggacctg tgccccgtat caaacagaga tgcgtccacg tttcggtcag caaattgcgt
    14701 ggggagaatc aaacgccatt gtatttgcca actctgtgat cggcgcacgg acggaacgat
    14761 accccgacat gttcgatgtg tgctgtgcca tcacaggccg cgctcctgcc atcgggctac
    14821 acttgacaga aaaccgagcg gggaagatcc tgctcaaact cgaaggggtt tcggcggaat
    14881 tacaggcaag tgacgacttt tacccagtac tcggtcatta cttggggaaa atctcattgg
    14941 acagaatccc cgtgatcgag ggaatgacca ttgtcccaga tgacgatcaa cttaaggcat
    15001 tgggtgcagc agcagcttct tctggtgcag tggcactctt tcatatggtg ggtgttaccc
    15061 ccgaagcgcc gactgtggaa gcggcctttc agggaaataa accagaggaa accttgatcg
    15121 tcaacatgga tactttgagg caggcgcgca gggaattgac ccatgctgat gatgccagcc
    15181 tcgacatggt tgtattgggc agtccgcatt tttctttggc ggaatataaa atccttgcga
    15241 aattggttga ggggaagaag aaacacgcag atgtgaagtt tctagtcacc tccagcaggg
    15301 caatgaccca gcttgcacag caggcagggt ttcttgaccc cattctgcaa tttggcgcgc
    15361 agatcaccgt agatacctgc atcctcacta caccgatgct gccaagggaa atccgctttt
    15421 tgatgacgaa ttcagccaaa tttgcgtact atgctccagg cttgctcgat aaaaggatag
    15481 cattcagcag cctggctgat tgtgttacct ccgctgtaaa aggcgagatc gttcgggatg
    15541 agacactatg gcaaagctag aactttttgg gaaacccgtc atatcaggca aggcaaacgg
    15601 gattgcactg gtcagccgtg agccgctttc attttggggc ggatacgatt ggaaaagtgg
    15661 agagatcacc gaccgcaggc atccgctttc gggtgaaaat gccagcggta aagtattagt
    15721 ggtgccattc acccgcggtt cgtcaaccac gaccgcagtt cttttggagg ccattcgcga
    15781 ggggactgcc cccgctgcaa ttatcacaac tgcaaccgat tttttcttcg ccctggcttc
    15841 tgtggtggct ggcgaattgt acgaaaaatc ctttccactg atcgctttgt cagaagcgga
    15901 ttttggcgcg ataaaaactg gagatcaggt taaagtgatg gaagatggga agttgatcgt
    15961 tgagtagcaa aacaacttct ggcattttgc aacttcacac gaaaaggagt cgttgatgag
    16021 ttggcttatc gttattttga tcgctgttgt tgtaaacatc attgtcctcg ccacttgcgg
    16081 cggaggcgga ggcttgatgc tgctggttgc gctcaatgga ttttctgaat cggctgcaat
    16141 gccgtttttg gcgggctatg gtctgttggt gctcgccatc agttttggaa ttgccacagg
    16201 cgcaacctgg atatatgtga aaccccgcag cacagcctct ggaattggat tttggcaggt
    16261 ggcaggtctc aacgcgggga ttatcgtcct gttgattttg ggggttgttg cgttcatggc
    16321 gatcagggca ctttgatact gcgatgatca ttgttgtaat gggtgtggca ggttcaggca
    16381 agaccaccgt gggcgcgttg ctcgcgctcg aacttggctg ggatttttac gacggcgacg
    16441 attttcattc cgatgccaat cgcgcaaaaa tgtcacagcg catcccgctc aacgatgaag
    16501 accgctccac gtggctggct gcccttcgag acctgatcaa ccaaaattta agtgagaatc
    16561 gttccatcgt gctggcatgt tctgcattga aagagtcgta tcggcacatg ctcaaggtca
    16621 atgagcaggt tcattttgtc cacttgcagg gaagttatca acagatcgaa gaacggctgc
    16681 gcgggcgcac ggggcatttt atgtctgcgg agatgcttgc cagtcaattc gatatccttg
    16741 aaacgccaga agacgccttg tccatcaata tttcaaacac gccgcaggaa attgtggcga
    16801 tcatttgcaa aggattgaat ccatgaaact cccatcacat ttatatctcg atgaaaagaa
    16861 catcccgtat gaagcgttga gcttttcgcc agaaacagaa aagggcgcag ccaatgttgc
    16921 acacgccctc ggtttttccg aacggcaggc agtgaagact ttgatttttc aagtggacac
    16981 tggtgagcgt gtactcgtga tgctgggtgg tgatcaaaac gcagtctcag gaaatttgaa
    17041 gaaagccatt ggttcgcgga atattcaaat ggctgcgcct gacgtcgtga aggagaccac
    17101 tggatacgtc atcggttcga tccccgcctt tcactggcag ccagaaaatt tcagatcttt
    17161 ccttgaagcg agtctcctta atgaacccat cctcggcacg ggtggtggtc aatggggtga
    17221 agagatcatg ataacgcctg aaaacctggt caaggccagc aatgccatcg tcgtcaacct
    17281 gacagaccga gaaaagcctg tatttttgga atcggtttag ataggactgc agttttcgaa
    17341 attacaagca caggtgattt gtgaagatga tcggaaagtc atttcctgcg aaaaagacca
    17401 tcgtactggt gattctgtca gtagcgggcg gacttcttgc agttgtaaat tttttcctgc
    17461 tttcgaaaga tatgctccgt ggagattatt cagcgaaaga tatttttcca cagccgatgt
    17521 atgggttctt ccgtgtgcct gacaggccga acaccatcgc acagaacggg gtaaacaggc
    17581 tggctgggga ttacgctcaa gtttattttc cgtcgctgga attctcttcc ctgacccaaa
    17641 attacgaaac gggatattta gacccgtgga aaagaccttc acgctatgcg ccgttcattc
    17701 attacgtgtg cgcactttcc ttctgcaaat tggattatgg gtatgcaagc cttttgcaca
    17761 tgcttactca aatggttctg ttctatgcgt tttttattgc cgcattcaag attttggaga
    17821 tcgaatcaga cctttgggtt ggcctgctga taaccaccct gcttctgttc gttacacctg
    17881 cggggttatc ctggttcgag cgcggacaat tttcactgta tgttgccctg gcatatttat
    17941 tgctgatgct ggggttgatc aaaaacaaac ctgtattcgt gtttatctcg gcgctgttcg
    18001 cttatgtcaa atggacatct tttccatttc tttttgtggt gttgttcgta cactggatga
    18061 gtgcaaaaaa cacaaaagag agagcccaat acacacaatc ggcgttgatc tatttgctgg
    18121 tgattcttgt gctttcgctt gcgttccgaa gtcgttttat tcatttcctt gagggtattt
    18181 acaaacagga agtcaatgtg gaggcggtgg gaattgcact ggcacagtta ctaccgacaa
    18241 gccttgtaaa aggcatgccg atcctcctca tcctgctggg gtatttatct ttgcgaaaaa
    18301 acaacatgga ttttgagcgc gccgccccat ttctgatcgg gtcgggaatt ttgatgctga
    18361 cctaccccac cgttgcattc gaatacaaca taccgaatct attctgtttc atcccactga
    18421 tcttttactg ggcgagaaat cccaagttaa tgaaccacgc tgcaaagtat gtttttttta
    18481 tcttcatttc tctggcttcc tgcacggcat ttcttggctc aacggtaggc gcaaaaacaa
    18541 ttttattcgg atacatggcc gtggcgggct tttttctaat tctgccattc attgacaaca
    18601 aaacgttcgg aaacgcgctg aaaggatgat ttaccaatgg caaatccgca aattcgaata
    18661 cagaccgagt taggggatat gattgtggag ctttaccctg cacatgcccc tataaccgtt
    18721 gagaattttt tacgctacgt tgacgagaat cgctttgaag gagccacatt ttatcgagtt
    18781 gtacatttgg aaaatcaacc caataatcaa gtcaaaatcg aagtaattca aggcggactc
    18841 aaggatgaag atcatccaca aagtctgccg aatattacac atgaaaccac aacccagacg
    18901 ggaattctgc acagggatgg cgtcatttca atggcacgga atgagccagg cactgcatca
    18961 gcggatttct tcatctgcac cggcgatcaa cctgagttgg atttcggagg caggcgcaac
    19021 ccagatggtc aaggttttgc ggcatttgga aaagtcattg cggggatgga aacagcacga
    19081 aggatccaag cgctgccaga agaaaatcag tatctgaaaa cggagatcgc gttcaaaata
    19141 cagcgtgccc aaccctgacg gtcatatttt ttaccagcgc ctcgcatggt taaaacaaaa
    19201 aacttgccac gtattctttc gtggcaagtt tttttctagc gatacgtcaa ccagttcagg
    19261 tctctcagct tccctgcaat atcttcgcgc aggtcatatt cacccatacg ccattcccag
    19321 ttaccgccaa gcttactcgg gaagttcatg cgggcttcac tgccaagatt aagcagatcc
    19381 tgcatcgggg taatggcaaa gacggcaacc gatttccaca caccacgaat caggtcccag
    19441 gcaaagtcat tgccatccac gctgaggtag cgttgtgcga attcacgttc gtgaggcgca
    19501 gcatgttcga accaaccgcg ggcggtgtcg ttatcgtgtg tgcctgtgta ggctacgcta
    19561 ttgggaatat agttatgcgg caggaaggga ttttcagggc ctgaaaaggc gaattgcaaa
    19621 accttcatac cgggcaaatc aaaagcggcc aataattcga tcacatccgg tgtgacaaga
    19681 ccaaggtcct cggcaatgat aggcaggcct gtgccatgcg tgatgaggcc atcaccaagg
    19741 aatttatcca cagcgcggaa caggtctttt ccggggccag tgacccaacg accaaattcg
    19801 gctgttgtgt gatccgcagg gatctcataa tacccagcaa aaccacgaaa gtggtcaaaa
    19861 cgcaaaatgt cgaagatctg caaagaggaa cggacacgtg aaagccacca ggcatagcct
    19921 tctttcttgt gaacatccca tttgtaaagc ggattacccc acaactgccc cgttgcagag
    19981 aaatcgtcag gcggtacacc tgccacaacg gttgggttgc cttcacggtc taaaaagaac
    20041 agatcgggat gcgaccagac atcagaagaa tcataggcaa caaagatggg aatatcgccg
    20101 atgatctgaa ttccgcgttc gttggcataa gcgcgtaact tgttccactg gcggaagaaa
    20161 atgaattgat aaaaagcaaa acgcatgaca ctttcgccaa gttccttctg cgctttgctc
    20221 aaggcggctt tctttctctt acgaagggct tcgggccaac cattccacgc accgccgccg
    20281 ttggcttcct tcaatgccat gaaaagagtg taatcatcga gccaggaggc attttcagcg
    20341 cagaaatagt caaaggcttg gcgtaaatgc tcagggttgg attggtagcg ggaaaatgcc
    20401 ttttgcaaaa gatccagctt ccttgggatg aacaggccaa agtccacgtg tgaagcgggc
    20461 aggtctttca tcccatcaag gtcttcctga gtcaataatc catctgcaaa aagttcgtca
    20521 gggctgatca aatatgggtt tcccgcaaat gcggaaaaag cctgataagg cgaatcgcca
    20581 taccctgtcg gcccgagcgg aagtacctgc cagagtttgc agcctgtcga tgcaagccag
    20641 tcgataaagc ggaaggactg cgggccaagg tcaccgatgc cgtatggaga tggcaggctg
    20701 gatggatgaa gaagaattcc agaagaacgt ttgaaggtca tgatttaatg agggagagat
    20761 atagattgag atattgtctt gctgaatttt cccaggagaa atcctgagcc atgcctgagc
    20821 gttgaagtga ttgccattgc tcgcggtttg caaaaacttt cagcgcattc ttgaccgccg
    20881 ccaccaagga caggtgatgc gctttttcaa aaacaaagcc tgttacaccg tgacgaacag
    20941 tatcattcaa cccgcctgcc gcacggacaa tgggcacaca gccgtaacgc attgcgatca
    21001 tttgcgagag gccgcaaggc tcataacgcg acggcatgag gaacatgtcg gagcctgcat
    21061 aaatctggcg cgcaaggcct gcatcgtaac gggtctcaac tttgaccttt tcagggaaaa
    21121 gcttttgcag tttcattgca gcttcttcga gttttgggtc gcctgtgcca aggataaccg
    21181 cctgccagtt caaacgcttc attgtgttga gcgcattaaa ggcaaggtca acaccttttt
    21241 ggacatccat ccgcgaaacg atggcgaaaa gcggaatctc aggatcctgc ggaagcccaa
    21301 gccgtgcttg taagaggcct ttgtttgcgg cacgtttttc gagggtttca agtgcaaaat
    21361 taacaccaat ggcagggtca tcagcggggt tgaacgagtc ggtatcgagg ccgttcaaaa
    21421 tgccgctcaa ggtttcgcgg cgcgattgca gaaattcatt caagccgcag ccgtattcct
    21481 cggtcagtac ttctttggca taggttggcg aaacgcccac aatggcatct gaagaccaaa
    21541 gccccaaagg caggggcatc acacgcgccc aatcaggcag atcggtttga gcgagtttca
    21601 ggccatagct ttccaaaata ccgctgacat ctggacccat gaatggaaga ttgtgcaacg
    21661 tcaataccga agcgacatgg cgggcacctt cttcccaacg tttggtcagg gaataataca
    21721 ggctcagagc cgtatgccaa tcattgccat gaacaacgtc agggaaccag ttgatctgcc
    21781 gcgataattc cagtgcggca atggaataaa aagaatattt ctcggcgtcc agtttggcat
    21841 ccaaagagta gaccgaacca ttggcacgaa tggcatcacc attaatgaag tagacaggca
    21901 ttcccttcag ggatgtttca aacgcatcca cctggacctc ggaattgcct ctcggaatcg
    21961 aaaaaatgcc tatcggcctg agattttcgg ctttgatggc aggatgatac ggcagcacaa
    22021 gccgtacatc cagtgtaatt tcatcgttgg aaagggcgcg caaagcgcgc ggcagagacc
    22081 ctgccacgtc acccaaccca cctactttga taaacggttc ggcttccgcc gcaagaaaaa
    22141 ggacattaat ggtttttggc atggcggtta ttttacctta cataaagacc ctaaaggcta
    22201 aataccttta gggtcttcca ggggtgcctg aagggtttcg aaccctcaac atccacatcc
    22261 acagtgtggc gctctgccgt tgagctacag gcaccatatt tagagcggcg aaattttacc
    22321 acaattcacc gcagattcaa caccgccaat gtctcgccgt catacatctc cccgggctct
    22381 tcaaaaccga ggctggcata cagcttcctt gcggattcgt tctctggctc ataactgatg
    22441 cccacattcc tgatcctttc atcggcacga agacgatcca gggctttcga cataatatcg
    22501 cggccatacc cttttccttg aaacttctca tccaccatta aacgggcaat aaaagcctga
    22561 aagcgcgagt gggtaaaatt caaacaatac atcaagaaac caacaggctc ttcacctgca
    22621 tacgcaccaa aagggaacat ctcccaatgc ccttcatcct caaaaccaaa ttgagcttca
    22681 gcaatggaat acagattaga agccacaaaa tgtgtctgat cttctcgtac cttcaaactg
    22741 atcagtgccc gccagttttc agcagaaacg ggatgaagag aaatcgtcac gtgatcatcc
    22801 aatcattttt cgaatctcgg aaaccaccgc ttcgcgctgc accgtgacct gctcgccagt
    22861 catcaggttt tttacggcgg cctgaccatt cgccgcttcg tctggaccaa cggtgacagc
    22921 gagcttgatc ttcattttgt cggcaaactt gaattgtttt tgcagcttcg caggttcggg
    22981 gaataccatg gcattgatcc cagcctcacg gagttgcgcg gccaactcaa acgatttcaa
    23041 ccacatggct tcatcaaaaa ccgtcaccaa cactgcggca ggcgttggaa taaattcagg
    23101 cacaagacca gcctcttgta aaacaatgcc cgccaccacg tcgcccatgg caaaaccaac
    23161 acctgaaagc ggctgtccgc caacatcaga cagaagatta tcgtaacgcc ctccgcccag
    23221 gatggcacgc ttcaatgaac cgctggtttc aaaagcctca aaaactgtcc ccgtataata
    23281 caagagacca cgcatgatgt tcgggtcaaa tttgacatac tcgctgacgc ccaaggcttt
    23341 caatgcagaa aacaggcgaa ccaactcctc gctatttttc cacagatcaa aatttccaag
    23401 gatgcccttc aacccatcaa gttgggtttg cgtcaggccc agttcaagtg cgtacgaatc
    23461 ccatttggca ggatccattt ttgtacgacg gtctaccagg ttggaaacat caaggcgcct
    23521 ttcgggagcc agaccaaggg cgtcgaattc agaatccatg aggcgccgat tatttacata
    23581 aatggcagcc cgctccgggg tgagtccgac cgaacgcagg aatgtcgccg caacagcaat
    23641 aagttccgca tccgcttcgg gagaattgac tcccagcatg tcaatattcc actgaaagaa
    23701 ctcacgtgta cggcctttct gcggctgttc gtaacgccaa catgggccga acgaccacca
    23761 acgcacaggg aaggttaact caccctgctt cgctgcaatc atacgcgcca ggctgggcgt
    23821 gagttcgggg cgcagagtca caaagtcacc gccgcggtcg gtgaaagtga aggactgctt
    23881 cttcaccagc tcctcccccg attttgccgc gtatagatcg atcgtctcaa taaagggtgc
    23941 gtcccattct tgatacccaa atttctgaga agccatgcga actttttcat aaatgaaatt
    24001 tcgcagcgcc atttgctcag gatagaattc tcttgtgcct tttacaggtt gtattttgct
    24061 cgccattttg actccagtgt gcaaatttta tcatgggttt caaatgcccc caaaattcag
    24121 cgattgaatt ttgattttgt tttggtataa tctcaaaacg tttattgcag tgcggacttt
    24181 ggttgtctgc actaacacga ttaaagttgt catacacaag gagataaaat gaaaattgat
    24241 tatcaagaac ccagcaaaga cctgctgatg cgcattgaca ttcacgaaaa atatggctcg
    24301 gccaatatcg acgtttggac gaacgacctg ctcaaacctc aggctggcat gaaaattctg
    24361 gatgtgggct gcggtgctgg caagctctgc ttccttttca atgaatttac aaaaggcggc
    24421 gcgcaaatta caggcggcga cttttcaaat gaattactcg ataaggcccg cgagaagaac
    24481 aaaaccgtcg gcgccaatat tgattttcaa tttttggatt tcaatcaacg ttttaacttt
    24541 gccgacaata cctttgattt gtgtaccagc gcatttgcca tttattacgc ctccgatctt
    24601 gactttacat ttggtgaggc ccaccgagtg ctgaatccag gcggacggct tttcgtctcc
    24661 gggccattgc ctgagaacaa gcaaatgttc tatgacatca tcaaggaagc cacaggcgct
    24721 cccatccccc ccatgcccgg gtcatcgcgc ttcaaaggtg atattttcaa caccattgac
    24781 aagatatttg ccaaaacaga actgcacaag ttcgaaaacc acctcacctt ccccgaagta
    24841 gccccgttca ttgattacgt ccgcgcttct ttgggtgaag accgcagact gtggacatcc
    24901 atgtttaacg gcaaagatga gtacgaagcc ctgatcggca agatcgaggc tgtggctaca
    24961 cgctggtttg agcgagatgg aaaactcgtc atgaccaagg ttgtcggcgg aattctcgcg
    25021 acgaaataaa ccaaatcacg acccgaagat aattgacgaa agccagttgt ttcgggtccc
    25081 cacattcacc atgaattttt tcggaaaacg agttctcttt ttaggcgccc accccgacga
    25141 tattgagttg gggtgtggag cgctgttgca caagatcgca aagaagacag atgtgttgtg
    25201 tgttacactc tctgacaatc agaaaaaccc cgacctgcaa aaagtaaaaa gcgaacattt
    25261 tcaatccatg caagtgttgg atgtgcctga agaaaagatc gtactgggac cattcaccac
    25321 ccgggttttc cctgatgcgc ggcaggaaat tttggagtac tttctgaaac tccgcaagga
    25381 tttcaacccc gacctgatct ttacccatac ccgacaggat gtacaccaag accacaacac
    25441 catgaccgat gaagccctgc gcgcctttcg tggaatcact gttttgggtt tcgatgttgt
    25501 gcggtcctct tacggctttt tccccagctt tctggtggag gtttctgaag aaaatgcggc
    25561 tgcaaagatc gaagcgcttt caaaatacga gacctaccgt gaccgttact atttcaacgc
    25621 agaattgacc cgttccatca tggttcggca cggagccctg gcagagcggc cactggcaga
    25681 aggatttgac attctgagga ttgtcggcaa attcggggag tgacgacata tcttgtttga
    25741 catcaattct tcaaaaccct ataatcaggc aaaatacccc tcaaccgccg caaggagaaa
    25801 aaatcatgca aaagtacgag cgggcagttt tcatttcata tgcttggggc gatgaacgcg
    25861 aagagatcgt caaccaactt gacaaggctc tgcaaaagcg aggggtcaag attatccgcg
    25921 acaaacgcga actggaatac aaggggtcca tttctgaatt catggagcgg atcggtcgcg
    25981 gcgactgtgt cattgtcgtc atcagcgata aatacctgcg gtcaccgaat tgcatgtttg
    26041 agctgatcga aattgctgaa aataaaaagt ttcgagagcg cgtttttccg atcattttgt
    26101 cagatgccaa tatctacgac cctgtcaaaa gagtggaata tgtcgatttc tgggaaaaca
    26161 aaagggatga gcttgccaag gccatccaaa aacttgaccc ggcaaacctg cagggcattc
    26221 gcgaagacat ggatctgtac gaccgtatcc gcgacatgat ttccgggctg gcaagcaccc
    26281 taaaagacat gaatgccctc tcgccacaaa cacaccgcga cgctgatttt gaagatctgt
    26341 ataacgccat tgagcaaagg ttggggcagc cccaatccgc ccgccccaaa aaaccgagca
    26401 aacccgcaaa aatagaaaaa gacgaaactg gcacaagaaa tatagaaata ggcggcagtg
    26461 caagcggcaa tgtgttcatt ggcgataaca acaagatcga aaaataatca gtcagaagtt
    26521 ccgtcttttt agaggtcagc atggcaaatg tttcaattgg cggcaatgta acagacagtg
    26581 ttttggtttt cggaaacaac aactacgttg tcaagatcgg ggatgtgaac ggcggcgtgg
    26641 tcaatatcgt caagccttcc gacaaaccca aggtctcggc acgccctgcc ccggtcatga
    26701 taaagccgcg cgcctttccc tctttaatgg accgtgtcga tgaagctgaa acggttaaaa
    26761 aggcggcaga gctggcagtc cctgtttctg tttggggaaa acaaggcatc ggaaagacct
    26821 ctttcatccg ccatttggca cacatccttc ccacaaacaa atatccaaat ggagtagtct
    26881 atctgaatgt ttccagcctt gggcatgaag acctgcttca agctttattc gatgcatttt
    26941 ttgaaagcga caacacttac aagccaacca cagctgacat ccttcatggg ctacagaaga
    27001 tctccgcact tatttttctt gatgatctgc aaattggccg tgatgaaacc atttccatcc
    27061 tgaacgcagc acccagttcc acttttatcc tgatctccag tgaacgcagt ttctgggggg
    27121 agggcgaagc ggttcaattg caggggctgc ctgttgggga ttcggtgcgg ttgttcgaaa
    27181 aagaattggc acgtccgctt gaaacggaag aaaaaatcac agtaccaaaa atctgcaaac
    27241 tgctgcaggg tcacccgctc agcatcctgc aatcggcttc actggcgcgt gaaagcggta
    27301 aaccccttga agagattctg aacggattga ccaatgaaaa gaaggatgac aagtccatgg
    27361 catacatggg gttggctagc cttacagact cggaaaaaca ggttattgcc ctgctggcag
    27421 ccgctggcgg aaccatcgtt ttggcagagc atatcaaggg catcttcaaa ggggacagcc
    27481 gtcagacaga tattcaaaag ctgatccagc tcgggtttgt acaggcacac agcccaaagt
    27541 tcagtgtgac cgaggcattc gcttcttccc ttgccgcgac ctgggatcta tcttcatggc
    27601 atgatatcct gttgaagtat tcgatcaagt ggttgagtca acagccagca tcggcattgg
    27661 tggatgaatc caccaacctg ctgatccaca ccgtcaaaac agcgggggaa agaaaaaaat
    27721 ggagcgaagt aatccaactt gggcgggcgc tcgaaaagtt tctggtctat tacaagcgct
    27781 ggcaggcatg ggccgatatt ttgaagttga tcatcgaggc cgccaaagcc ttgagcgaca
    27841 gcaaaacaca ggcctgggcc ctgcatcaac tcggaacccg cgccctgtat cttggtcaga
    27901 ccagcgaagc aaactcgctc ctggcacagg cacaaaccat ccgcaaagcc attggagaca
    27961 aagccggttt acgggttaca caacataatt tgaaaaccat caaaggtgga tcggggtgtg
    28021 caaaattcgt ttcctatttt gtcggtgctg ttgttggcgc caccgtactg acgggaatcg
    28081 cctggctcgc gatcaatgtt ctattttctc caccgatccc catcgcttta ccaactgcta
    28141 caaacacacc aaccttgcga ccgtcgtcga ccattccccc atcaaatacg cctcgaccat
    28201 cagaaacgcc gacagttcga ccgtcgagga cgccaagacc cacagacact ccaatgccag
    28261 tgctgttgta cgattttgtt gaaagcgcgc acgaggttta ttggtaccag accacaagta
    28321 attctgatgg tgagtttact caagacctgc catttttctt cgagccggtt gttccagatg
    28381 ttgaatcgta cctccctgga agtgacagcg cttacgttgg ttggtctttc gatacacaat
    28441 tgaccaatgg caacagatat gaaaaagtcc tgctcaccta cccgctttac gagcatttca
    28501 aggtcttcgg agattacaca gtaaagatcg gcagacctgc cccggaagca tacctcgaat
    28561 tgaccgccgg gtttaaggac attgttatcg aacctgtgga gggtgttatg ttcagggtgt
    28621 acgtgaatgg caaggtgtat attgaagagc catattactt tggcagcgaa ccgatacaga
    28681 cgggaagtcg aatccgactg gctgcaaaca cctatgaatt caggctcgaa gtggaatctc
    28741 tggatccatc tccgtacgat ttcgccactt gggcggttgt aaggctttgg gacaaagaac
    28801 cgtgagatta tttacttggc gctgacaaaa aaccccgcag gtgcttttcg cggtgacgca
    28861 aaatgtattt tagataccca aacaataaaa aaggacgttc tcggttgaga gcgtcctttt
    28921 atgatcttac ggcctattct ttcggcacgc caatgaaaat aattccatcc cgcacctgca
    28981 caggataagc agggatatcc accacagcag gcatggacat gaccttccct gtccgcacat
    29041 caaactcggc accatgccgc ggacagacaa tattatggtc ttcgatcacc ccatctccca
    29101 gcgggccatc atcatgggta caaatatcac ctatcgcgaa aaactggcca gctatgttga
    29161 aaataacgat cggcttgtca cccaaatcaa cgaacaggcg ctcgccattg ggcaattcgg
    29221 attcgggtgc gatctcaaga aattcgactt tggattcatc aagggcggtg taattaaaca
    29281 taaattcctt ttattnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcctt
    29401 ttattcaacc aaagagtcgc ttgcctcacc aagaccatac acacctgctt tcaaaacctt
    29461 caaagagagc aaggcgcatt tcaagcgcac agggcccaga tcaataccaa gcatttcaag
    29521 gacagaatcc ttgtttaatt ttttcacttc ttccagcggt ttgccgatga tggtttccat
    29581 caacaaatct gcggaggctt gagaaatcgc gcagccatga ccgtcaaaac gggcatcggt
    29641 caccaggccg cctgcgtcaa ctcgcaaatc gatctggata tgatctccgc aaaggggatt
    29701 gttatctgca aaagaaatgt catgcggatc gagctttccc cgatacgaag ggtgtttata
    29761 gtgctcaata attacttcac ggtagagatc gtccatctgt gctccaaaca ggcaaacctg
    29821 aaaacggtct acccaaacat ttccttcact ttgtagatcc cgttcaccag caaatccact
    29881 tcttcttttg tgctgtacaa atagaatgaa gcacgactcg tggcagggat gccgaatttt
    29941 tcatgcaaag gctgggcgca atgatgccca gcacggacgg caattccatc ctgatcaagg
    30001 atctgcgcca catcatgcgg atgcacaccc tcaagggtaa acgccgccac gccgcctttt
    30061 ttatcggcag atgggccaaa tagtttcaca ccggggatct cttccagccg ttcaagtgca
    30121 tactcggtga tttcatgctc gtgcgccgca attgcatcca tgccaacttt tgaaaggtag
    30181 tctacggctg caccaaagcc aatggcttct gcgatggcgg gagtgccagc ctcaaattta
    30241 tgcggcaggg tatttgcgcg gaacgaacgc agtttcactt ccttgatcat atcgccgccg
    30301 cctaaaaatg ggggcatcgc ctccagcaaa gcggtcttgc catacaatgc gccaatgcct
    30361 gtcggcccac acatcttgtg cgcggaaaaa gcaaggaaat ctgcatcaag agcctgcaca
    30421 tccactgtca aatgcggcac agactgtgca gcatccacca atgtgattgc acccactgca
    30481 tgagccaaac gaatcatctc tgcggctggg ttgatcgtac ccaaaacgtt ggacatgtgc
    30541 gtaaacgaaa ccagcttcgg gcgacggtca agaagcgttt tatacacctc aagatcgagt
    30601 acaccgtcct ccgtcacagg aataaattcc aactcgatac cgcgctccgc ttgcagcatg
    30661 tgccacggta caaggttgct gtggtgttcc atctcggtca ggatgacaag gtcaccctgc
    30721 ttcaagttgg cgcgcgccca actatacgcc accagattga tcgactcagt ggtgttgcgg
    30781 gtataaataa cttcgcgtgc cgatgcggca ttgataaatt tcgcgatccg ctcacgcgca
    30841 ccttcgtaca ttgcggtggc ttcttcagcc aaagcatgga caccacgatg aatgttcgca
    30901 ttcgaaaggc ggtaatactc gttcattgcc tcgatcacag ccaacggttt ctgcgaagtg
    30961 gcggttgagt ccaaatatgt caaaccgacc cctgcgcgta tttcacgctc aaggatcgga
    31021 aagtcttttc ttacctggga tacatttagc ttcatgatcg tttcgtcaac ttttagattt
    31081 ggcgactcgc tggcctaata ttctggaatg aaatccttca ccggcggctt ctttcccggt
    31141 acggggcgca tgtgctcgga attcaacggg aaccacaaaa tacggtcagg gaccatccaa
    31201 ccgtccgtga ggtacacatt tgaacggaac tgcacggcca ccattttgtt gtccacctgc
    31261 acaacgatcc ccttcaccca cccacgaatc cgttcgcgat tcttctcgtg gtcgcaaaac
    31321 acttccacgc ttccacccac ttcgtaatca tgctcggcac cgggtgcctt cataaaacta
    31381 aactgatttg tcctgctcgg acgggcaaca gggcggcggc gttccaccat ttgatttaca
    31441 acacgtttga tcaccggtgt aacggctggt ttcaccgctg tgggatggtt cgaggtttgc
    31501 tgttcaactt cctttttagg cgcagcagtt acaaccttgg aagcctgcat cggtttcttt
    31561 tttacttcag tcttgatcac ttttgttttt acggtcgttt tcgttttgac tgccacccgt
    31621 tttttcgaaa ctgtcctggc tgtcttggcc ttcgcactgg gctttaccat acgcttgctg
    31681 tttgatttca ctgccataac tcacatccta ttcttttgaa tggaagagac gccctccgcc
    31741 tctcacttaa ctgacaattg ccccggcaaa atcggcattc ccaccgccga taattttgcc
    31801 cgaaaacctg acaagcccca aagggacctg tcaggtttcg ttagattacg ctgatagctt
    31861 gtcctgaatc gcctgctgga acctttcacg cactccctca aatggaatac gctgcatgat
    31921 cgggtcaaaa aagccctcaa ccaccaaccg ttccgcctca cgctgcggga taccacggga
    31981 cttcaaatag aagagcggtt cctgctccaa cttgccgaca gtggccccat gcgtacagcg
    32041 aacatcatca gcaaggattt ccaaacctgg gatcgaatca gcgcgcgcct tgtcatcaag
    32101 gacaagatta cggttggcct gatacccatc tgtcttttgt gcgccggggg ccacatagat
    32161 catgccctgc cacacagagc ggcttttgcc tttcaaagca cctttgaaca gtaagtcact
    32221 ggtggtgtgc ggagcaagat gattctgctg ggtatcatgg tcgagatgct gggttccatc
    32281 tgtaaaataa aaacccgaca tacggcccac ggaaccttcc ccggcaaggt caaggtcaga
    32341 gaagtttttg gtcagacggg aacccactgc gccaaaaatc caatcgagat tgccgccgcg
    32401 ctccacgcgg gcgcgctcgt gcgaaaaatt ccacacatgg cgaccccacg actgcaattc
    32461 aacaaaacga aggttggcat ccttgccaac gtaaatttcg acaacacctg catgcatggc
    32521 atgccccacc tcatcaggtg aagccgcttc atggacatag gtcaccgagg cgccatcttc
    32581 cacataaaca agaagatgcg agaaatgagc cagttcagag cccgggcccc acaagacaga
    32641 atgcaaaggc tgttccacct gtacattcct gggaacatac agcaaaacac cattttgagc
    32701 caaagcagaa gcaagcgcgg cgaatttgcc atcatcaggt ttaacgatct gcccgatcaa
    32761 cttcgccagc aaatcggcat gatttttctc ggcagtgcgg aaatcagtga agaccacacc
    32821 ttttttcacc agagactcgt ccaagtccac tttggagccg ccggggagca gggtgatctg
    32881 tccgccatgc tgttcaccgg tcaacggctg gagcagatac tcgggcacgg caggcaggtc
    32941 atcaaaggct ccgatcttcg gcagcttgaa atcagaagct gggagtaagc gcaggtcggt
    33001 gcggcgccag gcttcgtctg tgtttacggg gagagaatgc ttgttgaatg cttcccaagc
    33061 cgaggcgcgg aaagaagcga ggctgcctgc agcggaaaca tctgcctgag taaaagcaaa
    33121 ttcctttgcg gcagtatctg cgcgtcgttg tcgtgttaca gtaacttttg gtttctcttg
    33181 catgttcaat tccgtttcaa agttggaaag ttcactggcc ggaagattca cctgccagca
    33241 atttaacttt ctaaccgata gacccttcca tttgtagttg aatcaaacgg ttcatttcga
    33301 cagcgtattc catcggcaac tctttgacga gcggctcgat gaaaccagaa acaaccatgg
    33361 ttgtggcttc ttcttcgctc aagccgcgag acatgaggta gaaaagctgc tcctcgccca
    33421 ctttgctgac agaggcttcg tggccgatgg tgacgtcatc ttcatcaatt tcgatgtagg
    33481 gataagtatc agaacgcgat ttggggtcga gcaacaacgc atcgcaaacc acattggatt
    33541 tgacaccctt ggcaccttta tacactttga gcaagccgcg ataggaagca cgtccgccat
    33601 tcttggaaat ggatttggaa gtgatcttgc tggtggtgtt cggcgcgaag tgaaccatct
    33661 ttgaacccgc atcctgaatt tggcccgtgc ccgcaaacgc catcgaaagc atttcgccgt
    33721 gcgcgcctgg ttccatcaga tagcaggaag gatatttcat ggtgactttg ctgccgaggt
    33781 tggcgtccac ccattcgtgg gtgccatttt caaaaacctt ggcacgctgg gtcacaaggt
    33841 tgtacacgtt cgttgaccag ttttgaatgg tggagtaacg ggaacgcgca ccctttttga
    33901 caataatctc gatcacacca gagtggaatg agtcagtcgt gtattgaggg gctgtacaac
    33961 cttccacgta atgcacctgc gcgccttcat cgacgatgat gagcgtgcgt tcaaattgac
    34021 cgacattggc ggtgttcaag cggaaatatg cctgcaaagg cagatccact tttacacctt
    34081 tgggtacgta aacgaaagaa ccgcccgacc aaacagcaga gttcaatgcg gcaaatttat
    34141 tatcttcaat cggcacaacc gtgccaaaat attcacggaa caattcgggg tgttggcgca
    34201 agccatcttc gatggacagg aagatcacac cctgtttctc aaggtgttct tgaatcgagt
    34261 ggtacaccat ctcagactcg tattgcgcgc caaggccggc caaaaacttt tgttcggctt
    34321 ctggcacacc cagcttgtca aaggtgcgct tgatatcatc gggaacatcg tcccatgatt
    34381 tttcgctttt ctcggtgggc ttgacgtaat aataaatatt atcaagatca agcgagctga
    34441 gttcaggtcc ccaattgggc atggggcgcg ctagaaaatg ctcaagcgcc ttgaggcgaa
    34501 attcaagcat ccacttcggc tcgcccttca tgcgtgagat gttttccaca acctcacggc
    34561 tcaacccttt ttcagactta aaggtgtagt tgtcatcgcg gtcgtgaaaa ccgtatttgt
    34621 attcgccgat ctcatcgagg attttagtat cttcagacat tcgattcacc attttctgga
    34681 tgacgctccg cttcacacgg acctgtcacc tgctactgat aaacaaacag actctaccct
    34741 tcggcaactg cgtcgtattt ctcgcggacc cagtcatagc cttgatcttc aaggtgaagt
    34801 gccaggtctg gcccgccaga ttcaacgatg cggccattca tcatcacatg cacgaagtgc
    34861 ggtttgatgt aattcaaaag gcgctgataa tgtgtgatga ccagcacccc aagttcaggc
    34921 ccagaaagag cgttgacgcc ttcagaaacg atgcgcaggg catcaatatc caaacctgag
    34981 tcggtttcat ccaaaactgc aaagcggggt tttagggttg ccatttgcag aatctcggcg
    35041 cgtttctttt caccacctga aaaaccctcg ttgaggtaac ggcctgcaaa attatggtcc
    35101 attttgagca tatccatctt ttccttcagc attttgcgga attcggggat gggcatgcct
    35161 ttatcttcgg gattttcagc gcgacggcgg gaattaattg ccgagcgcag gaagtttgcc
    35221 acggttacac cgggaatggc aacagggtat tggaaagcaa gaaaaactcc cgcacgggaa
    35281 cgttcatcag gttccatttc aaggatattt tcaccatcaa gcagcacttc gccgctggtg
    35341 actgtgtaat tggggtgccc catcaatgta taagccagtg tagatttacc tgtaccgttc
    35401 ggccccatga ttgcgtgagt ttcgccctgt ttaatcgtta gtgaaagccc tttcaaaatt
    35461 tctttatctt caatgcttac gtgaaggtcc tttatttcga gctgcgccat tcttttactc
    35521 ctgaaataag ccatttctgg cgtttttgat taagttggcg gcattatagc agggttgacc
    35581 gaaattgtca aatatttatg atatttttct agtatattcc cttttggcac aataaacacc
    35641 ccgcaaatca attcacctaa ccaaatacaa tggcaag
//
LOCUS       DAOQJC010000031        34494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_549_length_34494_cov_10.542495, whole genome shotgun sequence.
ACCESSION   DAOQJC010000031 DAOQJC010000000
VERSION     DAOQJC010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 34494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34494
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_549_length_34494_cov_10.542495"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLE39_06455"
     CDS             <1..188
                     /locus_tag="PLE39_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68002.1"
                     /translation="ILPMVTGILPWLTPLAAVGLTVIQLLALFAVHLPKKEPIVPNII
                     LGALSVFIVVGRWGLFS"
     gene            252..1283
                     /locus_tag="PLE39_06460"
     CDS             252..1283
                     /locus_tag="PLE39_06460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNE68003.1"
                     /translation="MQNIEFGLDTFGDVPMDDSGKPVSYARAIRQVVDEAVLADSVGV
                     DVIALGEHHRVEYSISTPETVLAGIATKTNRIRLASGVTVLSSDDPVRVFQRFATLDA
                     LSNGRAEVILGRGSFTESFPLFGYDLRDYEVLFEEKIELFAELLKEKPVTWSGTKRAS
                     LENVDVFPKTESGHLSTWVGVGGSPESVIRTARYGLPLILAIIGGNPQRFAPYIDLYK
                     RAAAEFGTTPQAVGIHSPGFIADTDEEAWDLYYPQARVVRDRIGRERGWPPSNLQEYQ
                     RERETGSLYVGSPETVARKIARVVKALGVNRFDLIYTAGTQSISSRLRAVELFGTKVI
                     PMVREILAG"
     gene            1331..2035
                     /locus_tag="PLE39_06465"
     CDS             1331..2035
                     /locus_tag="PLE39_06465"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HNE68004.1"
                     /translation="MKDSIYTMNIHTIGILGAGKVGTVLARLALSAGYRVLIGGSGDP
                     AKIALTIEVLTPGAVAVTAQEAASQADVVILALPLGKYRNIPVEALQGKLVIDAMNYW
                     WEVDGIRDDLTDPRSSSSEIVQAFLRESRVVKAFNHMGYHDLEEGAVPAGTSGRRAIA
                     IAGDDSNDVNTVAKLVDALGFDPVIAGPLAEGMRLGPGTEAFGANVGADELRAILERF
                     PESERGKVILQARMVL"
     gene            complement(2167..2733)
                     /locus_tag="PLE39_06470"
     CDS             complement(2167..2733)
                     /locus_tag="PLE39_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE68005.1"
                     /translation="MLPNPDEKLDPRVRRTRGLILSSFESLLAEKNFEGISVQDVTEK
                     AQINRATFYAHFPDKYALLDYSMSLMFMSEIEKRTLNVCTYSPDNLRNLILAVCEFLS
                     HLHTGCAQPHQQFESLVEGTLKKQIFDLLSYWLRKSKLPTEIPATVATWGIYGLASHY
                     SHLKKRPALEKFVDEAYPLVAVNLEQFA"
     gene            2868..3512
                     /locus_tag="PLE39_06475"
     CDS             2868..3512
                     /locus_tag="PLE39_06475"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNE68006.1"
                     /translation="MNITIIGSGNMGRGIGTRAVAGGHSVTFVDANPEVAEKAAADVK
                     AAAKSGAKVSTASLGDAELGDVVVLAVWYGTNIEIAKQLGSKLAGKVVVDIANPLNST
                     YDGLATAPDSSSAEDLAKALVAGAKVVKAFNTTYAGTLMAGEVAGQKLDVFIAGDDAD
                     AKAKVAQLVTDGGLRAVDTGPLSRARQIEGMQLLHIVLQGSLGTNWGSALKILG"
     gene            3611..4522
                     /locus_tag="PLE39_06480"
     CDS             3611..4522
                     /locus_tag="PLE39_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE68007.1"
                     /translation="MNAQTNAAKTEFSIHPATKLGLVSLTVANLENQILFYTQALGFK
                     VHWREGNRAGLGAGGADLLLMTEESNLKRYRGVTGLYHFAVLFPNRRELARAVARLSV
                     LKYRNHPTDHIMTKTTYLDDPEGNGIELYCESPEDGSFVIENDDFVTRRTDGSWSDGR
                     EPLDVEALFSHLKDDDRLDDPLPAETRVGHVHLHVRNVQEAVDFYHGIIGFDVMGNSS
                     KFQAAFISAGGYHHHLGLNAWKGEGAPPPPADAVGLRHFTVELPNQQALDEVVKRIDD
                     AGIPSNKTDQGLLLYDPSQNGVMLKVG"
     gene            complement(4809..7298)
                     /locus_tag="PLE39_06485"
     CDS             complement(4809..7298)
                     /locus_tag="PLE39_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNE68008.1"
                     /translation="MTHHRAKNIFIAIFLVLFLFGLRGSLAERAQAAPAQAVTAFLYP
                     PYPGTASQESVFDHTSPNYVADNRVVTFNGAEGKKTCPNPPPAGTPPPEAGVCDIGYG
                     GYWSYKLGDWLFYDGHDGIDYGMSYRPLYAAADADQVIYSGWWDPQNHKVSLGLYVRL
                     HHPNGYSTSYGHMSAVAVSACSTPGCVFIPHGEWIGFSGSTGNSSGPHLHLRVVNPSG
                     KAVDPYGWTGSGTDPWPSNQKESLWVQYPSLIYYGAAIFPSGSALGYPPAPVNGIVVD
                     DGGAGFSESPAACWKTGTATNATNGAMRYVKPRTSTATCSAQWEFPQGSTAGMYSVYI
                     RVPSNHATVEGAFYDIQHAGKTARVLINQAVYPNRFYAADGWIYVGKYNFTGTGSEFI
                     RLTNQTQDESAAIADMELGADSVQFIYQGDATPTPPSIVTDTPTPTRTLTGTPTKTFT
                     PTITRTPTATRTATITRTPTITRTPTITRTPSKTPTATNTKPATATPLYTKVKLYFVN
                     RFRLKANTPPYEVFGTRYLSSSSNLPEGVLKEYFKGPGSTEYGWGYTAIYNGFTGFNK
                     LEIVGDVAHVHLKGACVSSGKDFNIADLITLNLKQFGTIQSVKIYDQFDQTQNPSGAG
                     DSEPICLDPSFTATATASITPTASRTPTPTKTPTVTRTPTITKTPTNTRTPSRTPTPT
                     NTKLPSATPAYTKVTVYFVNKSKYNANTPPFEVEGKRYASSSTDFAKFVLDEYFKGPG
                     LTEKNDYGWTAVFSGFTGYSKFEVKEGVAYVYLKGTCNSAGRDYNLADILMVNLKQFS
                     SRIQFVKIYDENGSTQIPDGASDSIPACLAP"
     gene            complement(7319..7996)
                     /locus_tag="PLE39_06490"
     CDS             complement(7319..7996)
                     /locus_tag="PLE39_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68009.1"
                     /translation="MRKLITLLSIFFITACAAQNAAMSAPAQKVEAQQEFESPTAEPP
                     TMTATALLETETATATLVPPAMTPTETPLPPLELPTPLANPPALLAWDGTPTYLGDSK
                     PDYLFRVRYDPEIWALTTDSYGYPAIGHRGIEYCVIAPAFGRGLPSNTTVEHDIRKIG
                     VVEFEINTVFRGGVRQSVTYLGGDTNIYTGFEVTFAENADLCLQEAEMVFATFTSVKV
                     TQATPVP"
     gene            complement(8067..9128)
                     /locus_tag="PLE39_06495"
     CDS             complement(8067..9128)
                     /locus_tag="PLE39_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68010.1"
                     /translation="MKSHISALISFILLLSSCSPQPPAKNILAAIRLDPPALIQFSED
                     LTPLGELPISLPADCGIYDIFSPSRGSTLAVEISCSFGQALLFLDTETGSVTQAFPQG
                     DSHLLAWASNGKSVFLRVDNASDPRILRVNAETLAAEIIPINEFTYDLAPKPESFDFT
                     FTYSRGFGFGSEIQLAKDDGRIVQLLYADPLNYISFARWSPNGKQIAFIKIPDSQTPF
                     TVGELWVMDADGSNPRKLTDADAGHGYAANWSPDGKRIAFIVRENAEDEAANVSADAL
                     ISNLYVVDVTTDELTQITNLSEGRIETAHWSPDGNALFFLRVLNGKMELQTAPSDQLS
                     AEIKALLAEPACCLSWMRK"
     gene            9195..9959
                     /gene="surE"
                     /locus_tag="PLE39_06500"
     CDS             9195..9959
                     /gene="surE"
                     /locus_tag="PLE39_06500"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNE68011.1"
                     /translation="MHILVTNDDGVQAPGLLALAKEMRKLGKVTVFAPDKNWSASGHV
                     KTMERPLRVREVLLADGSPAFASDGAPSDCVALPLLGLIEKQVDLVVSGINPNANLGH
                     DVTYSGTVTAAMEAVIAGVKGIAVSLDSPEGFKGKLDYSTAAIVARRVAKKVMADGLP
                     EGVVMNVNVPYLKKEKLKGYMITRQGLRVYRDALDERVDPRGKPYYWIGGEAPTGVDE
                     PGTDFGALRAGYVSITPLQLDLTHYKAMDVLRKWKF"
     gene            9982..10602
                     /locus_tag="PLE39_06505"
     CDS             9982..10602
                     /locus_tag="PLE39_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HNE68012.1"
                     /translation="MTQYFFVDESGDAGLAGQIGSSSHFVVVMVQLPERTKVNPLVEL
                     RDSLNLSPHFEFKFHQSKPWQKERFFNELLNFPFRIRAAIVDKSQADFHPQEISPRQL
                     IIDLIVGLTMRADELDVSNDILIVDGATPTFCRDLRVSFTEQCRQKKRIRPFKNIVGA
                     NSKNEDGLQIADMVAGAIRLHSIGALDGYFHTLLPKIVDLWHFPEK"
     gene            10773..11054
                     /locus_tag="PLE39_06510"
     CDS             10773..11054
                     /locus_tag="PLE39_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68013.1"
                     /translation="MDFFKKLFSGTPAKAQKRYHTFSVKCLRCGEVVEGRIDLDNELS
                     VEYESNRDVYYGRKVLMGSGRCFQRMEVELKFDYNRQLLDQQVSGGEFV"
     gene            11054..11521
                     /locus_tag="PLE39_06515"
     CDS             11054..11521
                     /locus_tag="PLE39_06515"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HNE68014.1"
                     /translation="MDESIVDHYWYSFLHSLPPDSRYFGKLYVAERFGDNSALAQELG
                     QLVVDGVKTGTCSAIWEWEAEGKSIPQPGLITIVLDGNDQPMCIIETIEVFACRFKDV
                     DEEFAEAEGEGDRSLEYWRSEHKKYFSRVLPKIGREFSEDMPLVCERFKVVYK"
     gene            11521..13041
                     /locus_tag="PLE39_06520"
     CDS             11521..13041
                     /locus_tag="PLE39_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68015.1"
                     /translation="MRRLSNILLILLAGLLVVQAGLSLQWPIAHDEAPLLYEAFLMHS
                     EGRVPYRDLFDFQMPGSFAAYYLLGRLSGFNGFFRLRLLDLGILAALLAITFLFMRRF
                     GRQTAFAAAILFGLKYMQGGAFMSLQREYLLLGFVALAVWVSMRDVLTYKHRLLIGIL
                     FGLSAVIKPHAALGLLPILLFDITSLIQRQKLTLIKSAMTSTPPVAIGFAMPILAMIG
                     WLAATNALTPFLDIVLHYVPLYAQINGQMEITEAGTRIPFLLNQSLRLGGHALWVIPA
                     ALGVYLVPQENKRQAWLLAGLAACYAVYPAFSGQFFEYHYIPFIYFIVILAALALTEQ
                     QPLSTFHSPPSFLFPLSSLILLFAIAFAVRPSNTFLRQIQNRPIAKTTDRAEEIASYL
                     KENMQAGDTVQPLDWTGGTLLAMLETRAPLATKYVFDFYFYHHISTPYIQNLRADFMD
                     ELQEADPRFIVEVIAVDKPWLTGSDTSREFPELRAFLKANYSVTILEDDYLIYKRK"
     gene            13102..14667
                     /locus_tag="PLE39_06525"
     CDS             13102..14667
                     /locus_tag="PLE39_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68016.1"
                     /translation="MKHLFRITYLYLTIPFIIFCMGWLRLSVAIPVVAVIVWALWQLL
                     KQTPPGQDSKITIHHLLLALLVTGIWLFLSGVGGYAFQNWDHHWRNAVFRDLVTNNWP
                     VIYSSPERGPITMLVYYVGDWLPAALFGRVFGWSIANFMLFLWTWLGVLLTVLHLNLK
                     LKTSLVRSALLLIFFSGLDVVGALLFAKEYPTLWPPITHLEIWSGGDLQYSSFTTQLF
                     WVFNQAVPAWLCIVLILGDNVIARRVLSPTTQSSIHKEIASGIEQDRPRYDIKFFIWA
                     LSFFFAPLASIGLMPYLMVDFFTQADWRSFFKSIRVDLLFAAGIIFLLSYFFFSSNTA
                     AQERGFHSLVLKDWLFFFLLDGGIFWLMLAPFKWRDPRWAVTGILLLVMPFIQLGNGR
                     DFVMRASIAPLFYLMMMVGETIFKNTTARGLRFAFYVLLFLGALTPLYEINRSAVRTY
                     EYYFVLDETQRAQPNVEPATHLEKGGAPELEHPNAILADDIYTLTFMQDKLSKNFIAN
                     VRQSLYYRYLSSR"
     gene            14695..15063
                     /locus_tag="PLE39_06530"
     CDS             14695..15063
                     /locus_tag="PLE39_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNE68017.1"
                     /translation="MTQQFPSLPNPQAFYAEVWNIVCLIPRGKVASYGQIAKMIRPPA
                     GVDPETYLEFGALWVSNAIAASPNEVPWQRVVNSKGEITERDGLEAKRHKLLLEDEGV
                     PFGIRGRIDMKKYGWSGKSK"
     gene            15115..>15390
                     /locus_tag="PLE39_06535"
     CDS             15115..>15390
                     /locus_tag="PLE39_06535"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNE68018.1"
                     /translation="MPSFSSPPNPQAYYEQVWAITRLIPRGKVATYGQIAKMIPPPNG
                     VELEAYAAFAPRWVGGAMANCPDDVPWQRVINSQGKISERPGAERQRP"
     assembly_gap    15391..15490
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15491..16675
                     /locus_tag="PLE39_06540"
     CDS             <15491..16675
                     /locus_tag="PLE39_06540"
                     /inference="COORDINATES: protein motif:HMM:NF021832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNE68019.1"
                     /translation="GACIIQGDHKTFIPSVPTTELDPTGAGDTFCGATLAYLAQGAHP
                     IMAAQHAVPLAAQMIAHVGPAALLFNEPAPEIPASPKAQTNPTQIQKISKMVAALDEA
                     APFNFISPELPPAHHPKALDYFFAVTLQQFSFWNEQNNRYHTPLIAPLGGVSQKGAFY
                     LFDAYRRRLESDADFCSPERQANLSRAELLEIFKADDGTDPMPALDLHLEMANAYGRD
                     MLALHLTPEAVLQKARASANPLATLISILDKIGGYKEDPLRKKSGLLALILNQRPEAF
                     LTFAAGEQVEPVIDYHLMRSCLRVGLVDILDEDLKTRITNRELVSPAEEWAIRHTAYL
                     AIEEVVALSGKSTGAVDWFFFGARKRCPEMTEPECSLCQIDSVCAHRKELFQPVLRTT
                     FY"
     gene            16735..17106
                     /locus_tag="PLE39_06545"
     CDS             16735..17106
                     /locus_tag="PLE39_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68020.1"
                     /translation="MQSPEKPTKFGTISFILAIVVVVLWCVYFILFAAMTEGGATFGM
                     DGETAGYALVLGGGTVMAVLTVLITLAGIILGILALRKQDPKRGLAITGLVLNFLCFA
                     PYCLLLLLIAFGSISTADFIP"
     gene            17369..18673
                     /locus_tag="PLE39_06550"
     CDS             17369..18673
                     /locus_tag="PLE39_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68021.1"
                     /translation="MRKWRGTFYVLFLAVLMMLLSSCAPKVTLTYRVSGTATKALVEY
                     RGVDGEKTQESVTLPWEKSMEVGEDFRYELYVMNENEGGDIQCEISADGISFGTAEGS
                     MYTGCSGVVRTDGKTTSSFMTTLNDTYPDLSDLPYKEQGRFLIYTSQKFDMGEIVIKD
                     LKTGEEMQMTDGLRGGYCLSKSPVNAKVAFTSLRDSVNGDIYILDINARKGEELTNIT
                     NTPDTYEMCPSWSNDGQMVVFTFKSSETSPWEIGKIGYYGGDVSQITNSTAGENSLYP
                     AWSPDGAKIAYVVNNEDGDTVYVMNADGSNPAPLSETFASAFSVSQVAWSVDGQQLAF
                     FQHNDDLVSLFILSADGNVEKRIEMDVARTGSLSWDAEGKQIVFHAWKNISESADIYS
                     INLESGEIMILAENPTLDFLGPFLNTDDTITSFPRRTIELAQ"
     gene            complement(18756..19724)
                     /gene="holA"
                     /locus_tag="PLE39_06555"
     CDS             complement(18756..19724)
                     /gene="holA"
                     /locus_tag="PLE39_06555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNE68022.1"
                     /translation="MPNIFLLYGNDEFAISRKLKDFESDFTDPTTADMNTTRLEARTM
                     TENELNNAVNAMPFLAPKRLVFISGPSGKFNNPSTRKKFLEFLEKAPDTAKIVLFETI
                     EPKEAEKHWLTKWAEKNKPQVKPLGFFLPKQRDMPGWIINEAKNLGGQIDPPAAARLA
                     EMVGVDTRQAGMELSKLLAYVNWARPVHGSDVEAVCIVTSQQSVFDFVDALSQGNGKS
                     AQKLLHRLLENEDPFSLWGMVVRQFRLLIQAREILDGRGNKDDVARALGVHPFVAEKT
                     TGQAARFTMESLESIYHRLLGIDERVKTSQITLDLALDTLVVELAR"
     gene            complement(19758..20204)
                     /locus_tag="PLE39_06560"
     CDS             complement(19758..20204)
                     /locus_tag="PLE39_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68023.1"
                     /translation="MSTETPTNFRVNIHWALQLFLLVLCIPVIWGLIYLLTKMFAAIS
                     VAGSIEIVFLLVFLWFLVSFAFSAIEVNHSSISLHAPFGTTSINWDDVEIITLKGELF
                     TIQTKGKKKHLSVSMEIAGKGKKEFYEFINKRIESERIIVQKQSKH"
     gene            complement(20220..20705)
                     /locus_tag="PLE39_06565"
     CDS             complement(20220..20705)
                     /locus_tag="PLE39_06565"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HNE68024.1"
                     /translation="MILDLPFILETRRNHALEHATLHMLAHTHTGTMAGHSNPTGFFL
                     IGNFSTQDVWAAATEALERLRGGESGLAIHAGCGTNMATTALLAGTFAWSVLRLAKST
                     LMKILLMPLAVFLGVVGFLISRPLGPVLQQRITTEANMGNMQIIDIIPIRKGVHRVIT
                     K"
     gene            complement(20753..21748)
                     /locus_tag="PLE39_06570"
     CDS             complement(20753..21748)
                     /locus_tag="PLE39_06570"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNE68025.1"
                     /translation="MNTKASPLQVPSFHIREVPIHGDTILAPMDGYSDWPFRSLCREL
                     GSAMSYTEFIKVEKILSRSKQPAKRMYFTEPERPITFQIYGDDPDLILRAALQIQENN
                     PDVIDLNMGCPAKTIADRGAGVGMMPTPIKIARTFRKLVKHLHVPVTGKIRLGWDRKK
                     NYKLIARIVEEEGGSLIAVHGRTKEQRYAGNADWDAIAEVKSLVKIPVIGSGDVKTVA
                     DIDRMKAHTNVDAVMIGRGAIPNPWIFSRLDRDQVPPEMLKQTIRKHLARCVEFYGDG
                     DGSRLFRKNAVQYVMMQHLTRDERKEILKTRPSAEFLELLEKIYDSPIIPQPAIP"
     gene            21926..23365
                     /locus_tag="PLE39_06575"
     CDS             21926..23365
                     /locus_tag="PLE39_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNE68026.1"
                     /translation="MNRKILVAIFLLIFAFAQVGNASAATVEGTATLVELPTFSNIFD
                     VDLEWSVSGMPGGATQNIFVWGRQQAAPSVAYDPSDCRLAASLPGVSDAAGTYTYQYF
                     FSAFDQQTWEFTLTVDDAVDCNSATLPSTNSVMGSTFIDGWWLDGFIANPPSLDPAFF
                     EEIPVACNTFELWGLVTDRVPDRVAKTHGGEASTLGYSGFASWNQSITGTFTPPAPLG
                     PPDALLSWVYTFPSTASGLWSFSVDPTDYAGNNWGTTNFRYSFISEEELADCVTFSDV
                     SETDTEIYVRYMADLDLAHGYLDGTFLPDATLTRAEMAAFIELANGIQGDDPEFATPP
                     PSGSACDFSDVSSDDWFAGWVWQACADGYMNGIGNGLFDPNNYLTRGQVVTVLDNINK
                     MGTGSGYLATQNVFQGQWGNLYREAVFLDVAVGDFYAGPVQRAFGVGVVDGTSDTTFS
                     PDQAILRGEFIKWMYRTLSRIQPGCVECQ"
     gene            complement(23414..23965)
                     /gene="msrA"
                     /locus_tag="PLE39_06580"
     CDS             complement(23414..23965)
                     /gene="msrA"
                     /locus_tag="PLE39_06580"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNE68027.1"
                     /translation="MNTNYETITLAGGCFWCLEAVFDEMKGVLSVDSGYSNGHVPNPS
                     YRDVCSGTTGYAEVVQIKFDPTVISFRDVLNVFFAIHDPTSLNRQGADVGTQYRSGIY
                     YHTPEQKAAAEQAISELNAQGIWSSPIVTEVEAVHNFHIAENYHQEYFVRNQFQPYCQ
                     AVVAPKVAKFRKHYLELLKKQPA"
     gene            complement(24114..25163)
                     /locus_tag="PLE39_06585"
     CDS             complement(24114..25163)
                     /locus_tag="PLE39_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68028.1"
                     /translation="MNKQELLKLADYNFQRGNRELAKKYLVELLTLHPEDEPAWMLLA
                     RVVEERERQVECYRKVLKINPGNNEAKIALARLESKTQPLLVSTYQQQKAAAAKAPAR
                     NVLRGVMIAVAVLLGLGATTFAIASRNPQSNMAKLIIPASPTPYTQTLAGDVAPQTRA
                     DIVKKYPEYAPLVDTLISFAISNAESGMVGAPERPGAEIMPSDTAASQAKEAFESALP
                     QPGTLNTATLSEEQITSWLAVEMRNSPDLPLQDVQVYLRDGKIQIWGVVKGSADSTSA
                     LMVGSVSLDANGLPGFEIESMQIGQQVIPDVLVTQAESWLNQMLLDEINRQVPGLRIM
                     NINISSGMITVAGMR"
     gene            complement(25214..27700)
                     /locus_tag="PLE39_06590"
     CDS             complement(25214..27700)
                     /locus_tag="PLE39_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNE68029.1"
                     /translation="MAGMERFTQRARRVLSLAHQEAERARQNNIGTEHLLLGLMDEEG
                     GVAGRVLRDLGMTPERVREVVQRVSGSAVNFDPNRVELAPETQQVLEFAVDEARRLSH
                     HYIGTEHILLALVRVESTGLEALRRLGVTADQVRRQTRRVLNESASSSSPTPAGPQPG
                     KGGAANASPKTPLVDQLASDLTSKAEEKKLDPVIGRQKEIERVIQILARRTKNNPALI
                     GEPGVGKTAIVEGLAQRIVDGDVPAPLMNKRVLQLDVASLVAGTMYRGQFEERLKRII
                     DELKQSGAILFIDEVHMLVGAGAAGSSVDAANILKPALSRGELQVIGATTMDEYRKHI
                     ESDAALERRFQSVQVDEPSEEETIQILKGIRSAYEEHHHLVISDEALEAATHLSSRYV
                     TERFLPDKAIDLIDESSSRVRMYKSPAAKHAKDLFGQLRQARQNRTLAQEEGNSEDIK
                     EWEERELDLNQQIERLRTGWDRTTSPVVSAEDIAEVVSMWTGVPLMQLAEAESQRLLK
                     MEDELRKGIVGQEDAIIAISRAVRRARAGLKDPKRPIGSFMFLGPTGVGKTELTKSLA
                     KFMFGSEDAAIQLDMSEFMERHTAARLVGAPPGYVGYEDAGQLTEALRRRPYSIVVFD
                     EIEKAHPEVHNMLLQIMEEGHLSDAKGHKVDFRNAIIVMTSNIGADMIKNQTSLGFAM
                     KRDEATEERLSYEDMRKKLNESLKRAFRPEFINRMDSVVIFRSLNKEDIQKIVSLELS
                     KVSDRLVEHNLVLTASEEALSSLATMGYDSEFGARPLRRVIQQRVEDPLSDRLLAGEF
                     INGDTIFVDVNDDGEVVLNKAAEKQPEPSV"
     gene            complement(27952..28635)
                     /locus_tag="PLE39_06595"
     CDS             complement(27952..28635)
                     /locus_tag="PLE39_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE68030.1"
                     /translation="MFTPVQISRMFLKSYFTILNRSFDVDGNLPMPNGPKIIAMNHTP
                     GFDPLYLPFLLEDTPHFLLQDGMFRIPVIGWMLKGSGQIPVYRGTDKAKEAKEQACAI
                     LRNGGTIAIFPEGQDVELGKRIPAKTGVVRMALETGAPIIPLGLYTSPQNLTRLNFQW
                     EGTPRSGAWQFSGKSYMRIGTPWNPNAQADIHAQTEELMNRIYTLVAEAEVDSHFERH
                     TLLKPIHQW"
     gene            complement(28646..29305)
                     /locus_tag="PLE39_06600"
     CDS             complement(28646..29305)
                     /locus_tag="PLE39_06600"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNE68031.1"
                     /translation="MKKIFTIILVTAVLIAAWAFRVPAVNLLGWFGNREAVVNSIQGL
                     GLWGPFVLIVLLVLQVFLAFIPGQALMVACGYIYGFWGGLLISWIGLFAGGQAAFFLA
                     RHYGRPFAERWIAPDVLSKWDKAAAGQGIVFFTLSLVLPIFPNDAMCYVAGLGNITSR
                     RFTAANSLGRGLACLFMSAVGAFGSQLGWQAWVVIAILVILTCLGWLAFKNLKPEFLN
                     A"
     gene            complement(29442..31172)
                     /locus_tag="PLE39_06605"
     CDS             complement(29442..31172)
                     /locus_tag="PLE39_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68032.1"
                     /translation="MLFQATVLVDITAMAICLWMAFYLFARGFPSRVTMRGVILLLSL
                     SAFFFGAYNNIFNQITGTAALRAAFLSIGLGAWYDITLQLLPVENRKSRLPWTISLYT
                     LGLISIILLLSTPNSFIGEQGNQIYVARMGIGLPYILYAVFETATCFGIFKNLFSHRK
                     IGMTAQGKFFLVASIFPAVSVLYGALALALAPPLPRLIQDLLIFSGVFLMGLSVANHQ
                     TLVERRTTLQDFPITSLTILVITSIYAVISLRFGLPLQLVGPLAAFVVLTHALYDLVR
                     ESLERLRIRKEEMLRKQLHKLKSGQTPEDTLHLRLQEGLDLLCSTLDTSSGFIAIKRG
                     GEFVVTASRLSVEIDARFSSSLVSYEDIVQDQNDQIPNIIYFAPSFDGQIQIAIVGVG
                     KPNDRLDYSRGDLDLLSEVADQIGTIVSLNAPQVQPTQTEIDESGSTLAAAGMIDAIE
                     INPDQDFIKMVEEGLRHLSDYIALGKSPLADRLGVSAGSHIERGRELQQTIVAAIDLL
                     KPSEKRPPEPLPRVWYNHAVLHDAYVEGVPNREIMARLYISEGTFNRTRRNAIRGLAR
                     LLMEKKTTAK"
     gene            complement(31237..31566)
                     /locus_tag="PLE39_06610"
     CDS             complement(31237..31566)
                     /locus_tag="PLE39_06610"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HNE68033.1"
                     /translation="MNFNDYQSKSRVTAKYPAIGHGVVYPTLGLVNEAGEVAGKIKKV
                     FRDKSGEISAETREALKAELGDVLWYLAQTCTELEISLDEVAEANLAKLLDRQARGKI
                     QGDGDNR"
     gene            complement(31744..32319)
                     /locus_tag="PLE39_06615"
     CDS             complement(31744..32319)
                     /locus_tag="PLE39_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68034.1"
                     /translation="MQPESFSQKETEQPGMSLIQETKPAQRNPTPVILTVFLIIFLAA
                     TAGLGYWAYTLNSSLIATQQQLAELQSDHTKLQSENENLTAELAQVKADLEESSKDLT
                     NTQTKLEKAQAENLELNVKIENASKKLQILYAFSTIATPTDILAIDTLIKETKDRQLL
                     ERWNEFYSNPSESAGVNFILNLITAIQTSLK"
     gene            complement(32471..33376)
                     /locus_tag="PLE39_06620"
     CDS             complement(32471..33376)
                     /locus_tag="PLE39_06620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE68035.1"
                     /translation="MKIISRLYEGPNDFQTMLNLLAKIRPAARMNDFPVKVDIEENMA
                     NEEIRANTRLWFEGDRPIGWAYVDDGNNLRWEVEKQFEDSVGVEMVAWGEACMRSRRK
                     LNTGETSTLDASCREENHERLEFIRKHGFHQTDVISVGMKRLLSEPIPEPELPHGFSI
                     RAVKGTEEAEAVASTHRSAFGTEYMTTENRLIIMNSSEYDPSLDLVVIAPDGSIAAYC
                     TCSVNANGEGYTDPVATHPRYQRLGLAKALLLKGMQLLKERGMKSASLGTNGDNIAMQ
                     RTAEAVGFRLEHKTLWFEKEVNQID"
     gene            complement(33453..>34494)
                     /locus_tag="PLE39_06625"
     CDS             complement(33453..>34494)
                     /locus_tag="PLE39_06625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HNE68036.1"
                     /translation="RETMKLDAALPIVGLKDIPAIAEAAQAIGFDALWTQETQHDPFL
                     PCTLIAEHSETLRFGTAIAVSFSRSPANLAYTAWDLAAQSNGRFILGLGTQVKAHIER
                     RFGMAWPESVTGKLREQIQVIRAFWDCWQNGTKLNFRGEYYKATLMSPFFQPPPLPSP
                     IFGENGRGLGGGIPIYIAGVNAGLAKLAGELCEGFHAHPFNSSRYMNEVMLPAIEEGL
                     KKEGRKRQDITVSMTPFIATSPEEESFARMQIAFYASTPSYKSVMDFYGWGATAEKLS
                     GFAAKGEWAEMPMLITDDMLNEFCLMTTQENLAVDLKKRYAGIADRLTLYTPFVPGEK
                     DDWWRNLAKAFN"
BASE COUNT         7690 a         8513 c         9307 g         8884 t
ORIGIN      
        1 tgattttgcc aatggtcact ggcattctgc cgtggctgac gccgcttgcc gctgttgggc
       61 ttacggtcat tcaattgctt gccctgttcg ctgtgcattt gccgaagaag gaaccgatcg
      121 tgccgaatat tatattgggt gcgctgtcgg tgtttattgt tgttgggcgt tggggacttt
      181 tctcctaagg aaacaagggt taatgtagtc tacaatacca gcaactatta cctcacatgg
      241 agaaccgcgt tatgcaaaat attgaatttg gattggatac cttcggcgat gtgccaatgg
      301 acgactctgg caaacccgta tcttatgcga gagcgatccg tcaggtcgtt gatgaggctg
      361 tattggcaga ctccgtcggc gtggatgtga tcgcgctggg cgaacatcat cgcgtggagt
      421 attcgatctc gacgccagag acagtgctgg cgggcattgc cacaaagacc aatcggattc
      481 ggctcgcttc gggcgtgacc gtgctgagtt ccgatgaccc tgtgcgcgtg ttccagcgct
      541 ttgccacgct ggacgcgctc tcgaacgggc gggctgaagt catccttggg cgcggttcct
      601 tcactgagtc ttttcccctc ttcggttatg acctgcgcga ctacgaagtg ttatttgaag
      661 aaaagatcga actgtttgcc gaactgctca aagaaaaacc cgttacgtgg agcggcacca
      721 aacgtgcatc gcttgaaaac gtggatgttt tccccaagac cgagtcgggg catttgtcca
      781 cttgggtggg tgtcggtggt tcaccagagt cggtcattcg tacagcgcgt tatgggcttc
      841 cgcttatcct tgccatcatc ggtggcaatc cgcaacgctt tgccccttat attgatctct
      901 ataaacgcgc cgcggcagag ttcggcacaa cgccgcaagc cgttggaatt cactcaccag
      961 gcttcattgc cgacacggat gaagaggcgt gggatttgta ttacccccaa gcgcgggtcg
     1021 ttcgtgaccg catcggcagg gagcgcggtt ggcccccgtc caatttgcag gaatatcagc
     1081 gtgaacgaga aacgggttcg ctctacgtcg gttcgcccga aacggttgcg cgaaagatag
     1141 cccgtgttgt caaagcgctt ggtgtgaacc gcttcgacct gatctacacc gcaggtacac
     1201 agtccatcag ttcacgttta cgtgcggtgg agttgtttgg cacgaaggtg attccgatgg
     1261 tgcgcgagat tcttgcaggt taagcctgac cgttgaccgc ggtcgaccgt caacggctgt
     1321 cgtccaagat atgaaagaca gcatctacac tatgaatatt cacaccatcg gaattttagg
     1381 cgctgggaaa gtaggcaccg tgcttgcgcg acttgctttg tcggcgggat atcgcgtact
     1441 gatcggcggt tcaggcgacc ctgcaaagat tgcgctgacg atcgaagtcc tcacccctgg
     1501 cgcggtggca gtcactgccc aggaagccgc gtcacaagcg gatgtggtga tattggcact
     1561 tccgcttgga aagtaccgca acatccctgt cgaagccttg caaggcaaac ttgtcattga
     1621 tgcaatgaat tactggtggg aagtggatgg aattcgcgac gacctgaccg acccgcgtag
     1681 ttcatccagt gagatcgtgc aggcattcct gcgtgagtcg cgtgttgtca aggcattcaa
     1741 tcacatgggg tatcatgatc ttgaagaggg agccgtgcct gctggaacat ctgggcgtag
     1801 agcaattgcc atcgctggtg atgactccaa tgatgtcaat acagtagcaa aacttgttga
     1861 tgctctcggc ttcgaccccg tcattgctgg tccgctcgcg gaagggatgc ggcttggacc
     1921 tggcacggag gcattcggcg ccaatgttgg cgcggacgaa ttgcgggcga tccttgagcg
     1981 cttcccagaa tctgaacgtg ggaaggtgat tctgcaggcg aggatggttt tgtaataaaa
     2041 aatcttatgt gcagtagtta atttgtttat aagcagaccg ccgaccgaag actacagtct
     2101 tcggtcggcg gtctgccgtc agtaaatgtt atttttttcg tcgcttttct tggataccct
     2161 tgcggtttac gcaaactgtt ccaaattgac cgccaccagc ggatacgctt cgtctacgaa
     2221 tttttcgagg gcggggcgtt ttttcaagtg gctgtagtga gaagccaatc cgtaaatgcc
     2281 ccaggtggca accgtggcag ggatctcggt ggggagtttt gatttacgca gccagtaaga
     2341 gagcaggtca aaaatctgct tcttgagcgt gccttccacc agtgactcaa attgctggtg
     2401 cggttgggcg cagcctgtat gcaaatggga aaggaattca catacggcaa gaattaaatt
     2461 ccgcaggtta tcgggggagt atgtgcaaac gttcaatgtg cgtttttcga tttcgctcat
     2521 gaacataaga ctcatggaat aatccagcaa tgcatattta tcggggaagt gtgcataaaa
     2581 tgtggcgcga ttgatctgcg ctttttcggt cacgtcctgc acggagatgc cctcgaaatt
     2641 tttttcggcg agtaaagact caaaggatga cagaatcaag ccgcgggtac gtctgacgcg
     2701 cggatccagt ttttcatcgg ggttaggcaa cattttcact ccagtgttgg gtatccaaca
     2761 aatcaaaatg attgttggtt gattttatgg gcaagactgg taaactaagc aacatctgtt
     2821 gtttacacag caaccgtata ttatcactca taacaaggag agtcaagatg aacataacaa
     2881 ttatcggatc aggaaacatg ggacgcggta tcggaacccg cgcagtcgct ggtggtcact
     2941 cggtcacttt tgtggatgcg aaccctgaag tagcggaaaa ggctgccgct gatgtgaagg
     3001 ctgccgctaa gagcggtgca aaggtttcca ccgccagcct tggtgatgca gaacttggtg
     3061 atgtggttgt gctcgccgtt tggtacggca ccaacatcga aattgccaaa cagctcggca
     3121 gcaaactcgc tggcaaagtc gtggtggata ttgccaaccc gctcaactcc acctacgatg
     3181 gtcttgccac tgcccccgat tcttcttctg cagaagatct cgccaaggca ctcgtcgctg
     3241 gcgcaaaggt cgtcaaagcc ttcaacacca cctatgccgg tacgctcatg gctggtgaag
     3301 ttgctggtca gaagcttgat gttttcatcg ctggtgatga cgccgatgcc aaggcaaagg
     3361 ttgcccaact cgtgaccgat ggcggtctgc gcgctgtgga tacaggcccc ttgagccgcg
     3421 cccgccagat cgagggcatg cagttactgc acatcgttct gcagggttca ctcggaacca
     3481 attggggtag tgcactgaaa attttaggat aatgtaaaat ggagtgcggg gcatccccgc
     3541 actccattta ttgtttgtct catcggttga cgatgacttt tagaatgatg agacacttat
     3601 agaggaaaga atgaacgcac aaacaaatgc cgccaaaacg gaattctcga ttcatcccgc
     3661 cacgaaactt ggtctggtct cgttgacagt agccaacctt gagaatcaga tccttttcta
     3721 tacccaggca ctcggattca aagttcactg gcgtgaaggc aacagggcgg gccttggtgc
     3781 aggcggcgca gacttgctgc tgatgaccga agaatccaac ctgaagagat accgcggcgt
     3841 gactggcttg tatcactttg cggtgctgtt ccccaatcgc cgtgaactgg cacgtgccgt
     3901 ggcccgtttg tctgtgttga aataccgcaa ccaccccacc gaccacatca tgacgaagac
     3961 cacctacctc gatgaccccg agggcaatgg catcgaactg tattgcgaat cgcccgaaga
     4021 tggttccttt gtcatcgaaa acgatgactt tgtcacccgc cgtactgacg gttcctggag
     4081 cgatggccgt gagcccttgg atgtggaagc cttattctcc catttgaagg acgatgaccg
     4141 tctggacgat ccattgcccg ctgaaacacg ggtgggacat gtccatttgc atgtgcgcaa
     4201 cgtgcaggaa gccgtggatt tttatcacgg catcatcggt ttcgatgtga tggggaattc
     4261 atccaaattt caggccgcgt tcatttccgc gggcggctat catcaccacc ttggcttaaa
     4321 tgcctggaaa ggtgaaggcg ctcctcctcc ccctgccgat gcagtcgggt tgcgccattt
     4381 cacagtggaa ttgccgaatc aacaagcatt ggatgaggtt gtcaaacgca ttgatgatgc
     4441 aggcatccca tccaacaaaa cggatcaagg attgctgctg tacgatccat cgcaaaacgg
     4501 tgtcatgttg aaagttggat aagcaattat aaataaagta tggacacagt aacgctgtgt
     4561 ccatacttta ttaaaaagat ttgcagaaga tgaagattat attggacctc ttgcataagt
     4621 tgcagggaat gtctttgaaa agccattttg aaccacgaag acattgaagc agtgcggagc
     4681 ctcctgcgga cggagttttt aattgttttc tctgtgtctc cgtggttaaa ttttcagact
     4741 tttgcaagag gtctattgac catgtcaatg atttttactg atgtcttcca ttatgaatta
     4801 cagggatatc acggcgccag acaggcaggg atcgaatcgc ttgcaccgtc gggaatctgt
     4861 gtagacccgt tctcgtcgta gatcttcaca aattgaattc ggctgctgaa ttgttttaaa
     4921 ttcaccatca gaatatcggc gaggttgtaa tccctgccag cggagttgca ggtgcctttg
     4981 aggtagacat acgccacacc ctctttgacc tcgaattttg aataccccgt aaatccgctg
     5041 aacacggcgg tccaaccgta gtcgttcttt tcggtcaggc ctggaccttt gaaatattca
     5101 tccagaacga attttgcaaa gtcagttgat gaactggcgt agcgtttgcc ttccacttcg
     5161 aagggcggag tgttggcgtt gtatttggat ttgttcacaa agtacacggt cactttggtg
     5221 taggcgggcg ttgccgaggg gagttttgta ttcgttggcg tgggggtacg tgacggggtg
     5281 cgggtgttgg tgggtgtctt tgtaatggtc ggggtgcgtg tgaccgtggg tgtctttgtc
     5341 ggtgtggggg tgcgtgatgc agtgggggtg atgcttgcag tagccgtggc cgtgaaagac
     5401 gggtcaaggc agatcggctc ggagtcaccg gcgccagacg gattttgagt ttgatcgaac
     5461 tggtcgtaga tctttacaga ttgaatggtg ccaaactgtt tcaagttcaa tgtgatcagg
     5521 tcggcaatgt tgaaatcttt tccgctcgaa acacaggccc ccttgaggtg gacatgtgcc
     5581 acatcgccaa cgatctccag tttgttgaac cctgtaaacc cgttatagat ggcggtgtat
     5641 ccccatccat attcggtcga accagggcct ttgaagtatt ccttcaaaac gccttctggt
     5701 aaattggatg aggaggaaag gtaacgtgtg ccaaatactt cgtagggcgg tgtattggct
     5761 ttgagcctga aacgatttac gaaatacagt ttcacttttg tataaagcgg cgtggctgtg
     5821 gcgggcttgg tgtttgtggc agtgggcgtc ttcgatggcg tgcgggtgat cgtcggtgtc
     5881 cgtgtgatgg tgggagtgcg ggtgatcgtt gccgtgcgcg tggctgttgg cgtgcgggtg
     5941 atggtggggg tgaaggtctt ggtcggagtc cctgtcagcg tacgagtcgg tgtgggggta
     6001 tcagtaacaa ttgacggcgg agtcggagtc gcgtcgcctt gatagatgaa ttgaacggaa
     6061 tcagcaccca gttccatatc cgcgatcgca gcggactcat cctgggtctg gttcgtgagt
     6121 cggatgaatt cgctccctgt accggtgaag ttgtatttgc ccacgtagat ccagccatcg
     6181 gcggcgtaga aacggtttgg atacacagcc tgattgatga gcactcgcgc cgttttgcct
     6241 gcgtgttgaa tatcgtagaa cgcaccttcc accgtggcgt gattcgacgg cacacggata
     6301 tagaccgaat acattcctgc tgtgctgcct tgtgggaatt cccattgcgc cgagcaggtt
     6361 gcggtcgatg tgcgcggttt gacgtaacgc attgcgccgt tggttgcgtt ggtggcagtg
     6421 cctgtcttcc aacatgctgc gggtgattcg ctgaagcctg cgccgccatc atccacgaca
     6481 atgccgttga cgggagcagg cgggtagccc aatgcactcc ccgaaggaaa gatggccgcg
     6541 ccatagtaaa tgagcgatgg atattggacc cacagtgatt ctttttggtt gctgggccac
     6601 gggtctgtgc cggagcctgt ccagccgtag gggtcaacgg ctttgccgct tgggttgacc
     6661 acgcgcaaat gcaaatgcgg acccgatgaa ttgcctgtgc tgccgctgaa accgatccac
     6721 tcgccgtgtg ggataaagac acagcccggt gtggagcagg ctgagaccgc cactgcactc
     6781 atatgaccat aggaggttga atacccattc ggatgatgca ggcggacgta caagccaagg
     6841 ctgaccttgt gattttgcgg atcccaccag ccagaataga tgacctgatc ggcatcggcg
     6901 gcggcataca gcggacgata gctcatgcca tagtcaatgc catcgtgacc atcgtagaag
     6961 agccaatcgc cgagtttgta agaccagtag ccaccatagc caatatcaca cacacctgct
     7021 tctggcggag gtgtgcccgc gggcggcggg ttggggcagg tcttcttacc ttcggcgccg
     7081 ttgaaggtga cgacgcggtt atcagcgaca taattggggg aggtgtggtc gaaaaccgat
     7141 tcttgcgaag ccgtgccagg atatggcgga tataaaaatg cggtcaccgc ctgggcaggc
     7201 gcggcttgtg cgcgctcggc caatgacccg cgcaagccga ataaaaacag gacgagaaag
     7261 attgcgatga aaatattttt tgcgcgatga tgagtcacaa tgatttcccc aattcggatt
     7321 atggaacagg tgtggcctgg gtcactttga ccgatgtgaa tgtggcaaat accatttcgg
     7381 cttcctgcaa acacaggtcg gcgttttcgg cgaaggttac ttcgaagccc gtgtaaatat
     7441 tcgtgtcgcc gccgaggtag gtgacggatt gccgcactcc gccgcgaaag acggtgttga
     7501 tctcaaattc aaccacacca attttgcgaa tgtcatgttc cacggttgtg ttggagggca
     7561 gtccgcggcc aaaggctgga gcgatgacgc aatactcgat gccgcgatga ccgatggcag
     7621 ggtagccata gctatcggtg gtgagcgccc aaatttcggg gtcgtaacgc acgcggaaca
     7681 gataatcggg tttcgaatcg ccgaggtagg tgggggtacc atcccatgcg agcagggcgg
     7741 gcgggttggc aagcggtgtg ggcaattcga gcggcggcaa cggcgtttcg gttggggtca
     7801 ttgcgggggg cacgagtgtg gctgtggcag tttccgtttc aagcagggcg gtggcggtca
     7861 tcgtgggtgg ttcagcggtc ggcgactcga attcctgctg ggcttccact ttttgggcgg
     7921 gcgcagacat ggccgcattt tgtgcggcac aggcagtgat gaaaaagatg gagaggaggg
     7981 tgatgagttt tcgcattcag ttatttttta ccacgaaggg tcacaaagat acacaaagtt
     8041 tttgattttc ctttgtgggt aaagatttac ttccgcatcc aacttaaaca gcaggctggt
     8101 tctgcgagaa gggccttgat ctccgctgac agttgatccg aaggcgccgt ttgcaattcc
     8161 attttaccat tcaggactcg caggaaaaaa agcgcatttc cgtccggtga ccagtgtgcg
     8221 gtttcgattc ggccttcgct caagtttgtg atctgcgtca attcgtctgt tgtgacatcc
     8281 acaacataaa gattgctgat cagcgcgtcg gcagagacat tcgccgcttc atcttccgcg
     8341 ttttcacgga cgatgaatgc gatgcgtttt ccatcgggtg accaattcgc cgcgtagccg
     8401 tgacctgcat ctgcgtcggt gagtttgcgc gggttgctgc catcggcatc catcacccac
     8461 aattcgccca ctgtgaacgg ggtctgcgaa tcgggaattt tgatgaaggc gatctgcttt
     8521 ccattgggtg accagcgcgc aaaggaaatg taattgagcg ggtcggcata caaaagctgg
     8581 acgatgcggc catcgtcttt ggcaagttga atctcgctgc caaatccgaa tccgcgtgag
     8641 taggtgaagg tgaaatcaaa actttcgggt ttgggagcga ggtcgtaggt gaattcgttg
     8701 atggggatga tctcagcggc gagcgtttct gcattcacgc gcaggatgcg tggatcggag
     8761 gcattgtcca cgcgtagaaa aacggacttg ccgttggaag cccatgcaag caggtgactg
     8821 tcaccctgag gaaatgcctg ggtgaccgaa ccagtttccg tatccagaaa aagaagcgcc
     8881 tgcccgaacg agcaggaaat ttccacggcg agggtggatc cgcgtgacgg ggagaaaatg
     8941 tcgtagatgc cgcaatctgc aggaagggaa atgggcagtt cgccaagggg ggtgaggtct
     9001 tcggagaatt ggatcagcgc gggagggtca agccgaatgg cggcgaggat gtttttggcg
     9061 ggcggttggg gcgagcaaga ggaaagaagt aagatgaaag aaatcaatgc agaaatatgg
     9121 gacttcataa gggtattata atcatcgcac atttgtaaat ccaaaatcgt aaatctcaaa
     9181 tcggaaatcg actcatgcac atacttgtaa ccaatgatga cggcgtgcag gcgcccggac
     9241 tcctcgctct ggcgaaggaa atgcgtaaac ttggcaaagt gactgttttt gcacccgata
     9301 agaactggtc tgcttcgggg catgtgaaaa cgatggagcg gcctttgcgt gtgcgcgaag
     9361 tgctgcttgc ggatggaagc cctgctttcg cttcagatgg tgcgccctcc gattgtgtgg
     9421 ctttgccgct gcttggcttg atcgaaaagc aggtggacct ggtcgtttcg ggcattaacc
     9481 ccaatgccaa cctcggccat gatgtgacct attccggtac tgtcaccgcc gcgatggaag
     9541 ctgtgatcgc aggcgtgaag ggaattgcgg tttcgcttga ttcacctgag gggttcaaag
     9601 gcaaattgga ttattccacc gccgccattg tggcccgccg cgttgctaaa aaagtgatgg
     9661 ctgacggcct gcccgaaggt gtggtgatga acgtcaacgt gccgtatttg aagaaggaga
     9721 aactcaaggg atacatgatc acccgtcagg gtttgcgcgt gtatcgcgat gcgctggatg
     9781 agcgtgttga tccgcgcggc aaaccgtatt actggatcgg cggcgaagcc cccaccggcg
     9841 tggacgagcc cggtaccgat tttggcgccc tgcgtgcggg gtatgtctcg atcacgcccc
     9901 tgcaattgga cttgacccat tacaaggcga tggatgtgtt gaggaagtgg aagttttagg
     9961 atgtgaccgg ggcagagcct catgacgcaa tatttctttg tagatgaatc gggagatgcg
    10021 ggtttggctg ggcaaattgg ctcaagcagt cattttgttg ttgtaatggt gcaactgcca
    10081 gaacgtacaa aggtaaaccc gcttgttgaa cttagagatt cactcaacct atccccccac
    10141 tttgaattca agtttcacca gtctaaaccc tggcaaaaag aacgtttttt taatgaactc
    10201 ttgaatttcc ctttcaggat ccgtgccgca attgttgata aatctcaggc cgattttcac
    10261 ccgcaagaga tatctccccg ccagcttatc attgatttga tcgttgggct taccatgaga
    10321 gcagacgaat tggatgtttc caatgatatt cttatcgtag atggggccac cccaacattc
    10381 tgtcgtgacc ttcgcgtatc ttttacagaa caatgcaggc agaaaaaacg aatccgccct
    10441 tttaagaaca tcgttggggc aaattcgaaa aatgaagatg ggttgcaaat tgcggatatg
    10501 gttgcaggag caatacgttt acattccatc ggcgcattgg atggatattt tcacaccctg
    10561 ttgccgaaaa tcgttgattt gtggcacttc ccggagaaat aaaacacccc cgactattct
    10621 cttgcgagaa agacagcctc aggtggctac cttgtcgtcg ggggcgggta aaacggtctt
    10681 ccatccattg ctgggtgctt ccgttttatt ggtaaactca ttatacagag aatggaaact
    10741 gacgtcaaac ccttgtttta ggaaaaaaag aaatggactt cttcaaaaaa ctctttagtg
    10801 gcacccctgc aaaagcgcaa aagcgctacc ataccttctc tgtcaaatgc ctgcgttgcg
    10861 gagaggtggt cgaaggccgt attgacctgg acaatgaact gagcgtggaa tatgaatcga
    10921 atcgcgatgt gtattatggc cgcaaagtat taatgggcag cggccgatgc ttccaacgga
    10981 tggaagtgga attgaaattc gattacaacc gtcaattgct ggatcagcaa gtgagcggtg
    11041 gagaatttgt ctaatggacg aatcgatcgt agatcattat tggtattcat ttttacacag
    11101 cctgccgcct gattcgcggt attttggaaa actatatgtc gccgaacgtt ttggcgataa
    11161 ctctgcactg gcgcaggagc tcggccaatt ggtggtggac ggggtcaaga ccggtacatg
    11221 ctctgcgatt tgggagtggg aggccgaggg caaatccatt ccccagcccg gtctgatcac
    11281 cattgtcctt gatggaaatg accagcccat gtgcatcatt gaaaccattg aggtctttgc
    11341 ctgccgattc aaagatgtgg acgaggagtt tgcagaggca gagggggagg gcgatcgttc
    11401 gctggaatac tggcgcagtg aacacaaaaa gtatttttcg cgtgtgcttc caaaaattgg
    11461 gcgggaattt agtgaagata tgccgctggt ctgtgaacgt ttcaaagtcg tttataaata
    11521 atgcgtcgtc tctcgaacat tctcctgatc cttcttgccg ggcttctggt tgttcaggcg
    11581 ggcctctctt tgcaatggcc cattgcacac gatgaagcgc cgcttcttta cgaagccttc
    11641 ctcatgcata gtgaaggccg tgttccctac cgcgaccttt ttgattttca aatgccaggc
    11701 agttttgccg cgtattattt gctcggcagg ttgagcggct tcaacggttt cttccgcttg
    11761 cgcctgcttg atcttggtat ccttgccgcg ctgcttgcca tcacattttt attcatgcgc
    11821 cgttttggca gacaaaccgc atttgccgcc gcgattctct ttgggttgaa atacatgcag
    11881 ggtggcgcgt tcatgtcttt gcagcgtgaa tacctcctgc ttggttttgt tgcacttgcc
    11941 gtttgggtat ccatgcggga tgtcttgacc tataaacatc gtctgctcat tggaattctc
    12001 tttggcctat cggcagtgat caaaccccac gccgcgctcg gactcctccc catcctgctc
    12061 tttgacatta caagcctgat ccaacgccaa aagttgactc tgattaagtc tgccatgaca
    12121 agcactcccc ccgtcgcgat cggattcgca atgcccatcc ttgccatgat cgggtggctt
    12181 gccgctacaa acgcgttgac tccttttctg gatattgttc tgcactatgt gcccttgtac
    12241 gcgcagatca acgggcagat ggagatcact gaagcgggga cgagaattcc atttttgctg
    12301 aatcaatccc ttcgtctggg tggacacgcg ctttgggtga ttccagcagc gcttggtgtg
    12361 tatctcgtcc cacaggaaaa taagagacag gcctggctgc ttgctggtct tgccgcatgt
    12421 tacgccgttt accctgcatt ctcgggtcag ttctttgaat atcactacat cccttttatt
    12481 tatttcattg tcatacttgc tgcgctggct ctcaccgaac aacagccact ttccactttc
    12541 cactcaccgc cttccttcct cttccctctt tcttctctta tccttttatt tgccatcgct
    12601 ttcgctgtcc gaccgtccaa tacctttctc cgtcagatcc aaaaccgacc cattgcgaaa
    12661 accacagatc gagccgaaga gatcgccagt tatcttaaag aaaatatgca ggcgggcgat
    12721 accgtccagc cattggattg gacaggcggc accctgcttg ccatgcttga aacgcgcgca
    12781 ccgctggcta cgaaatatgt tttcgatttt tatttttatc atcacatttc aacaccttac
    12841 atccaaaacc tgcgcgccga tttcatggac gaactgcagg aggccgatcc gcgctttatc
    12901 gttgaggtta tcgccgtgga taaaccctgg cttacgggct cggatacctc ccgcgaattt
    12961 cctgaattac gtgcgtttct gaaagcaaat tattccgtta caatccttga agacgactat
    13021 ttgatttata aaagaaagta gtgcaggctt aaccctgcca ttccccgcag cggaccagag
    13081 tccgcgcgtt gcagcgaaaa catgaagcat cttttccgca tcacctacct gtacctcacc
    13141 atcccattca tcattttttg catggggtgg ctgcgcttgt ctgttgcgat tcctgttgtg
    13201 gctgtcattg tgtgggcgct ctggcaattg ctaaaacaaa ccccacctgg gcaagattcg
    13261 aaaattacta ttcaccattt actacttgct ttactggtca ctggcatctg gctcttcctt
    13321 tccggggtgg gtggatatgc ctttcaaaac tgggaccatc actggcgcaa tgcggtcttc
    13381 cgcgatcttg tgacgaacaa ctggcctgtg atctattcct cccccgagcg tggaccgatc
    13441 accatgctgg tttactacgt gggcgactgg cttcccgcgg cgctttttgg gcgggtcttt
    13501 ggctggagca ttgccaattt catgctcttc ctttggacgt ggcttggggt tctgctcacc
    13561 gttttgcatt tgaatctcaa actgaagacc tcgctggtca ggtccgcatt gctgttgatc
    13621 tttttcagcg gattggatgt ggttggggcg ctgctttttg caaaggaata cccaacgctt
    13681 tggccgccga tcactcacct tgagatctgg tcgggtggtg atttgcaata ctcctccttc
    13741 acgacgcagt tgttttgggt gttcaatcaa gctgtccctg cgtggctgtg tattgtgctg
    13801 atcctgggtg acaatgtcat tgcgaggagg gtgctttccc cgacgacgca atcctcaatt
    13861 cataaagaga ttgcttcggg catagagcaa gatcgccctc gttatgacat caaatttttc
    13921 atctgggcgt tgtcattttt ctttgcgccg ctggcatcca ttgggttgat gccctacctc
    13981 atggtcgatt ttttcacgca ggccgattgg agatcgtttt tcaaatccat tcgtgttgac
    14041 cttttgtttg ctgcggggat catttttctc ctttcatatt ttttcttttc ctccaacacc
    14101 gccgcacagg aacgtggttt tcactctctt gtgctgaagg attggctttt cttctttttg
    14161 cttgatggcg gcatcttttg gttgatgctg gctccattca aatggcgcga tccgcgctgg
    14221 gcggtgacgg ggattctgct tttggtcatg ccgttcattc aacttggcaa cggccgcgat
    14281 tttgtgatgc gggcttcgat cgcgccgctg ttttatttga tgatgatggt tggcgaaacg
    14341 atttttaaaa acaccacagc ccgcggtttg cgatttgcgt tttatgttct gcttttcctc
    14401 ggcgcgttga ctccgcttta cgagatcaac cgctcggctg tgcggaccta cgaatattat
    14461 tttgtgttgg atgaaaccca gcgcgcccaa ccgaacgttg aacctgccac acatcttgaa
    14521 aagggtggtg cgcctgagct tgagcatccc aatgccatcc ttgccgatga tatttacacg
    14581 cttacattta tgcaggataa actctcgaag aatttcattg ccaatgtcag gcagtctttg
    14641 tattatcgat acctgtcgtc gcgttgatct gatacctaaa aaaggaaaat tcccatgacc
    14701 cagcaattcc cgtcccttcc gaaccctcag gctttttatg ccgaggtgtg gaatattgtc
    14761 tgtctgatcc cgcgtggcaa ggttgcttcg tatgggcaga tcgcgaaaat gatccgcccg
    14821 ccagcaggtg tggaccctga aacctacctc gaatttggtg ctttgtgggt cagcaacgcc
    14881 attgcggcca gccccaatga agtgccgtgg cagagagtgg tcaactcgaa aggtgagatc
    14941 accgagcgtg atgggctgga agccaagcgc cacaaacttt tgcttgagga tgaaggcgtt
    15001 ccgtttggca ttagagggcg tattgacatg aagaaatatg gttggagtgg aaagtcgaaa
    15061 tagacgacag gccacgatca ctggtcaacg gtttactgtc gaaaggataa actcatgcct
    15121 tccttctcct ctccccccaa cccacaagcc tattacgaac aggtctgggc gatcacccgc
    15181 cttatcccgc gcggaaaagt cgccacctac gggcagatcg ccaaaatgat tccgccgccc
    15241 aatggtgtgg aacttgaagc ctatgccgcg ttcgctccgc gctgggtggg cggcgcgatg
    15301 gcgaactgcc ccgacgatgt gccgtggcag cgcgtgatca actcgcaagg taagatcagc
    15361 gaacgccccg gcgcggagag gcagcgtccg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15481 nnnnnnnnnn ggcgcttgca tcattcaagg cgatcacaaa accttcatcc cctccgtacc
    15541 caccaccgag ctcgacccca ctggtgcggg tgacaccttc tgcggcgcga cgctggcgta
    15601 tctcgcgcag ggagcgcatc ccatcatggc ggcacagcac gccgttccac tcgccgcgca
    15661 gatgatcgcg catgttggtc ccgccgcgtt gttgttcaac gaacccgcgc ctgagattcc
    15721 agccagcccc aaagcgcaga cgaacccaac ccagattcaa aaaatttcca aaatggttgc
    15781 cgcgctcgat gaagccgcgc ccttcaactt catcagcccc gagcttccgc ctgcgcatca
    15841 ccccaaggcg ctcgattatt ttttcgcggt cacgctgcag cagttcagtt tttggaacga
    15901 acagaacaat cgttatcaca cgccgctgat tgcgccgctt ggcggagtca gccaaaaggg
    15961 cgcattctat ttgttcgacg catacaggcg tcggcttgag tctgacgcgg atttttgttc
    16021 ccccgagcgg caggcaaatt tgagtcgcgc cgagctgctc gagatcttca aagcggatga
    16081 tggaaccgat cccatgcccg cgctggattt gcatctggaa atggcgaatg cgtacgggcg
    16141 ggatatgctt gcgcttcatc tcacgccgga agctgtgttg caaaaagccc gagcctcagc
    16201 caacccgtta gcgactctca tctccatcct cgacaaaatt ggcggataca aagaagaccc
    16261 gctccgtaaa aaaagcggac tgctcgcgct catcctcaat caacgccctg aagcattcct
    16321 gacctttgcc gctggcgagc aagtggaacc agtcatcgac tatcacctca tgcgctcatg
    16381 cttgcgtgtg ggtttggtgg atattctcga cgaagatttg aaaaccagaa tcaccaaccg
    16441 tgaacttgtc tcccccgccg aagaatgggc gatccgccac accgcctacc ttgctattga
    16501 agaggttgtg gcattgtctg gcaaatccac cggcgccgtg gactggttct tcttcggcgc
    16561 gcgcaaacgt tgccccgaaa tgaccgaacc cgaatgcagt ttgtgccaga tcgattcggt
    16621 ctgcgctcat cgcaaggaat tgtttcaacc tgtgttacga acgacatttt attaattgac
    16681 cgtggacagc gaacgataga ccatcatcca aaaactttat caaggagcca taccatgcaa
    16741 tcccccgaaa aaccgaccaa atttggaacc atctctttca ttcttgccat cgtcgtcgtt
    16801 gtgttgtggt gcgtttactt catcctcttt gctgcaatga ccgaaggcgg tgcaaccttc
    16861 ggcatggatg gtgaaactgc aggttatgca cttgttcttg gcggcggcac agtgatggca
    16921 gtgctgaccg ttttgatcac ccttgcagga atcatcctcg gtattctcgc actacgcaag
    16981 caagacccca aacgcggact tgccatcacc gggcttgtgc tcaacttctt atgctttgcg
    17041 ccctattgtc ttttgctgtt gttgatcgcc tttggcagta tctccactgc cgatttcatt
    17101 ccctagtatc ctttcaacca gttcccaaaa tacccagagc caggtttgga aaaacctggc
    17161 tctttgcttc ttatccatcc acatcctgca aatcacttta aaagtacgat gtcttttatg
    17221 agagtgtttt tataagcgaa gggctctaat tatggacatc gagattcttc gctttgcaca
    17281 ggatgacact tttgagaagc attcgctaaa aacgaaagct ctggggtaaa atgccgtttc
    17341 aaaaaaataa atgaggatga gaaaaataat gagaaaatgg cgtgggactt tctatgtttt
    17401 gtttttggct gttctgatga tgttactttc aagttgtgct cccaaggtga cacttaccta
    17461 tcgtgttagc ggaacagcca ccaaggcttt ggtggaatat cgcggggtgg atggggagaa
    17521 aacacaggaa agcgtaaccc ttccttggga gaaaagcatg gaagtcgggg aggatttccg
    17581 ttacgaattg tacgtaatga acgaaaatga aggtggagat atccagtgcg agatttctgc
    17641 tgatggaata tcgttcggta ccgctgaggg atccatgtac actggttgtt caggggttgt
    17701 tagaactgat gggaaaacga cttcctcctt catgaccacc ctgaatgata cctatccaga
    17761 tttgagcgat ctgccttata aggaacaggg gcgttttttg atttacacct cacaaaagtt
    17821 tgatatgggc gagatcgtga taaaagactt gaaaaccggt gaagaaatgc aaatgacgga
    17881 cggcttgaga ggtggatact gtctttcaaa atctcctgtg aacgccaagg tcgcgttcac
    17941 ctcactgcgg gatagcgtca atggcgatat ttatattttg gatataaacg ccaggaaggg
    18001 tgaagaacta acgaacatta ccaacactcc cgatacgtat gaaatgtgcc cctcctggtc
    18061 gaatgatgga cagatggtgg tattcacgtt caaatcatct gaaacctccc cgtgggagat
    18121 cggcaagatc ggctactatg gcggcgatgt ttcacagatc accaactcca cagcgggtga
    18181 gaattccctt tacccggcct ggtctccaga tggagcaaag atcgcctacg tggtcaataa
    18241 cgaggatggc gacactgtct atgtgatgaa cgccgatggc agcaaccctg ccccgctttc
    18301 ggaaacattt gccagtgctt tctcggtcag tcaggtggcg tggtctgtag acgggcagca
    18361 gcttgccttc ttccagcaca atgatgacct tgtaagctta ttcatccttt ctgcagatgg
    18421 aaacgtggaa aagagaattg agatggatgt ggcgcggaca ggctcacttt catgggatgc
    18481 cgaaggaaaa cagatcgttt tccatgcctg gaagaacatc agcgagtcag cagatattta
    18541 ctcgatcaac cttgagtcag gagagataat gatccttgca gaaaacccca cgttggattt
    18601 tcttggcccg ttccttaata ccgatgacac catcacgtcg tttccccgcc gtacaattga
    18661 actggcgcaa tagtaaggtt cattcaccct gttgctttgt cgcctgcaag cgctgaaaac
    18721 ggagtatgtg catcccccag tttgcatcgg ctgacttatc gtgccagttc caccaccaat
    18781 gtatcaagag ccaggtccag cgtgatctgg ctcgttttga ctctctcgtc aatgccgagc
    18841 aggcggtgat aaatgctttc aagcgattcc atcgtaaaac gcgcagcctg tcccgttgtc
    18901 ttttcagcga caaacggatg aacccccagc gcgcgcgcca cgtcgtcctt gtttccgcgc
    18961 ccgtccagaa tttcacgcgc ctgaatcaac aagcggaatt gacgcacgac catgccccac
    19021 aacgaaaatg gatcttcgtt ttccaaaagc cgatgcaata atttttgcgc ggattttcca
    19081 ttgccctgag acaatgcatc aacgaaatca aacacacttt gctgggatgt gacgatacaa
    19141 accgcttcca catccgaccc gtgaacgggt ctggcccaat tgacataggc cagcaatttt
    19201 gagagttcca tccccgcctg gcgggtatcc acgccgacca tttctgccag ccgtgccgcc
    19261 gcaggcgggt caatttgccc gcccagattt tttgcttcat taatgatcca gcctggcata
    19321 tctctttgct tgggtaaaaa gaatcccaac ggtttgacct gtggcttgtt cttttctgcc
    19381 cattttgtca gccagtgttt ttctgcttcc ttgggttcaa tggtttcaaa aagcacgatt
    19441 ttggcagtgt cgggggcttt ttccagaaat tccaaaaact tcttccgtgt ggatgggttg
    19501 ttgaatttcc ccgatggccc tgagataaag accagtctct tgggggcaag gaatggcatg
    19561 gcattgaccg cgttgttgag ttcgttttcg gtcatggtcc gcgcttcgag acgggtcgta
    19621 ttcatgtccg cggtggtggg gtcggtgaaa tccgactcga aatctttgag cttgcgcgag
    19681 atcgcaaact cgtcattgcc atagaggagg aagatgttgg gcattggagt tgaaaggcgg
    19741 aactttcgtg ctttgagtta gtgtttcgat tgcttttgaa cgatgatgcg ttcgctttcg
    19801 atgcgcttgt tgatgaattc gtaaaattct tttttgcctt ttcctgcgat ctccatcgaa
    19861 actgaaagat gctttttctt gccttttgtt tgaatggtga aaagctcgcc tttcagggtg
    19921 ataatttcca catcgtccca attgatggaa gttgtgccga atggcgcgtg caggctgatg
    19981 gacgaatggt tgacttcaat ggctgagaag gcaaaagaaa caaggaacca caggaacaca
    20041 agcagaaaaa cgatctcaat gctgccagca acggaaatcg ccgcaaacat tttggtcaaa
    20101 agataaataa gcccccaaat tacaggaatg cataaaacca gcaggaaaag ctgtaacgcc
    20161 caatgaatat taactctaaa gttggttggg gtttcggtgg acataaactg gattcctctt
    20221 tattttgtaa tcacccgatg cacacctttg cgaatgggga tgatgtcaat gatctgcatg
    20281 ttccccatgt tggcttcggt ggtgatgcgc tgctgcagca cagggccaag cggacgggag
    20341 atgaggaagc cgacaacgcc gaggaacaca gccagcggca tgagcaggat cttcatcagc
    20401 gtggattttg ccaatcgaag caccgaccag gcaaaggtcc cggctagaag cgctgtggtc
    20461 gccatgttgg tcccacaacc ggcatggatc gccagtccgc tttcaccgcc gcgtaatctt
    20521 tccagtgctt cagtggcggc cgcccagaca tcctgcgtgg aaaaattccc gatcaggaaa
    20581 aagccagtgg ggttggagtg ccctgccatg gtgccagtgt gagtgtgtgc cagcatgtga
    20641 agcgtggcat gttcgagcgc atggttgcgc cgcgtttcaa ggatgaaggg aaggtctagg
    20701 atcatggttt gattatacgt taaatcgata cgctcccgtc ggggtcgcgt tgttagggta
    20761 tggcaggttg ggggatgatc ggcgagtcgt aaatcttttc gagcaattcc aggaattcag
    20821 ccgaggggcg ggttttgagg atctctttgc gttcatcgcg ggtgaggtgt tgcatcatga
    20881 catattggac cgcgttctta cggaagagac gcgaaccatc tccatcgccg tagaattcaa
    20941 cacagcgggc aagatgtttt cggatggttt gtttcaacat ttcaggcgga acctggtcgc
    21001 ggtcaagacg cgagaatatc cacgggttgg ggattgcgcc ccgcccgatc atgacggcat
    21061 ctacattggt atgcgctttc atgcgatcaa tatcggcgac ggtttttaca tcgccgctgc
    21121 caatgacggg gatcttgacc agtgacttga cctcggcaat ggcatcccaa tcggcgttgc
    21181 ctgcgtaacg ctgttctttg gtacgtccat gcacagcaat gagcgagccg ccttcttctt
    21241 ccacaatgcg ggcgatgagc ttgtaatttt ttttgcgatc ccacccaagg cggatcttgc
    21301 ccgtgaccgg cacatgcaga tgtttgacca gtttgcggaa ggtgcgcgcg attttgatcg
    21361 gcgtgggcat catgccaacc cctgcaccac ggtccgcaat ggttttggcg gggcagccca
    21421 tgttgaggtc gatcacatca ggattatttt cctggatctg aagggcggct cgcaaaatga
    21481 gatcggggtc atcgccgtag atttgaaagg tgatggggcg ttcgggttcg gtgaagtaca
    21541 tccgcttggc gggctgtttg gaacggctga gaattttctc cactttgatg aattcggtgt
    21601 agctcatggc tgagccgagt tcgcggcaaa gggagcggaa gggccagtcg gagtagccat
    21661 ccatgggggc aaggatggta tctccatgaa tgggcacttc acggatgtgg aaagagggta
    21721 cttgaagagg ggaggctttt gtattcattt tccgttctgg aaagtataac agagtatgtt
    21781 ttggctgttt tttctgttaa tgggctattt tgtattcatt gagatttcgt tttggttttg
    21841 gtaaaaaggt actactttca tttttgaaag atataatgtc ttttaagtag acaagttttg
    21901 tattgttttt attgaggaga gaaagatgaa cagaaaaatc ctggtagcta ttttcctgct
    21961 gatttttgcc tttgcacagg ttggcaacgc ttcagccgcc actgtggaag gtacggctac
    22021 ccttgtagaa ctgcccacat tcagcaatat attcgatgtc gaccttgagt ggagtgtaag
    22081 cggaatgccg ggtggggcaa cccagaacat ttttgtctgg ggcaggcagc aagcggctcc
    22141 gtccgtggct tatgaccctt ccgattgccg tttggcggcc tctttgccgg gtgtgagcga
    22201 cgctgctggc acttacactt atcaatactt cttttccgct ttcgatcagc aaacctggga
    22261 atttacgttg acggtggatg atgctgtaga ttgtaattct gccaccttac cctccaccaa
    22321 ttcggtaatg ggcagcactt tcattgatgg ctggtggttg gatgggttta tcgccaatcc
    22381 gccctccctt gaccccgctt ttttcgaaga gataccggtg gcctgcaaca ccttcgaact
    22441 gtgggggctg gtcaccgata gagtgccaga ccgtgtagcg aaaacgcacg gtggagaagc
    22501 ctcaaccctt ggttattctg gctttgcttc gtggaatcaa tccattaccg gcaccttcac
    22561 tccgcctgca cctttagggc cgccagatgc cttgctttca tgggtgtaca ccttcccatc
    22621 aactgccagt ggtctgtggt cattctctgt agatcccacc gattatgctg gaaacaactg
    22681 gggtaccacc aacttccgct attcgttcat cagcgaagag gaattagctg attgtgtaac
    22741 cttctcagat gtaagtgaaa ccgatactga aatttacgtc cgctatatgg ccgatcttga
    22801 tctggcgcat ggttatttgg atggtacctt ccttccagat gccactttga cccgtgctga
    22861 aatggccgca tttattgagt tggccaatgg aatccagggt gatgaccccg aatttgcgac
    22921 cccaccccca tctggctcgg cttgtgattt tagcgatgtt agcagcgatg attggtttgc
    22981 tggctgggtt tggcaggcct gtgctgatgg gtatatgaac ggtatcggca acggtttatt
    23041 tgacccgaac aactacctca cccgtggaca ggtggtaacg gtcctcgata acatcaacaa
    23101 aatgggcact ggcagcggtt accttgccac ccagaacgtt tttcagggcc aatggggcaa
    23161 cctgtaccgt gaagcagtct ttttggatgt cgctgtcggc gatttctacg caggcccggt
    23221 tcagcgcgct tttggcgttg gtgttgtgga tggaaccagc gacaccacat tctcgccaga
    23281 tcaggccatt ctgcgcggcg agttcatcaa gtggatgtac cgcactttga gccgcattca
    23341 gcctggctgt gttgagtgcc agtaataggg aaaagaatca agaggcggac ccatttgggt
    23401 ccgcctcttt ttgttatgca ggctgctttt ttagcaattc cagataatgc tttcgaaact
    23461 tcgccacctt tggcgcaaca accgcctgac aatacggctg gaactggttc cgcacaaaat
    23521 actcctggtg ataattctct gcaatgtgga agttgtgaac cgcttccacc tccgtaacaa
    23581 tggggctgct ccagatcccc tgggcattca actcgctgat agcctgttca gcagccgctt
    23641 tttgttcggg ggtgtgataa tagattccag aacgatactg tgttcccaca tccgcaccct
    23701 gacggttaag actggtggga tcatggatcg caaaaaagac attcaaaaca tcacggaatg
    23761 agatgaccgt tgggtcgaat ttgatctgca ccacttcagc atatccagtg gtgccgctgc
    23821 aaacgtcacg ataacttggg ttgggaacat gcccattcga atatcccgag tcgaccgaaa
    23881 gcacaccttt catttcatcg aaaacagctt ccaaacacca aaagcatccc ccagccagtg
    23941 tgatggtttc ataatttgtg ttcatgataa aaactccttt ttattgtttg gatcacaatg
    24001 acaggcctgt ctgctgaata tgacgcgtcg aagacttgaa atattcttaa atgcatatga
    24061 tagtttaaga aaaaaagccg cccaatccgt gggcggcttt ttgtgtgatc ggtttatcgc
    24121 atcccggcaa ctgtgatcat gccgctgctg atattgatgt tcatgatgcg cagcccaggc
    24181 acctgcctgt tgatttcatc cagcagcatc tggttgagcc aggattcagc ctgggtaacc
    24241 agcacatcgg gaatgacttg ctggccgatt tgcatcgatt cgatttcaaa gcccggaagt
    24301 ccgttcgcat ccaaactaac actccccacc atgagcgcag acgttgagtc agcgctgcct
    24361 ttcaccaccc cccaaatctg tattttcccg tcacgcaaat agacctgtac atcctgcaat
    24421 ggaaggtcag ggctgtttcg catttccact gcaagccacg aggtgatctg ttcttcgctt
    24481 agtgtggctg tgttcagggt accgggctgg ggcagggcgc tttcaaaggc ttctttggct
    24541 tgtgaggcag ccgtgtctga aggcatgatt tcggcaccgg ggcgctccgg ggcgccgacc
    24601 atgccgcttt cggcattgct gatcgcaaag ctgatcaacg tatccaccaa tggcgcatac
    24661 tcaggatatt tttttacgat atctgcacgg gtctgtggag cgacatctcc agccaaagtt
    24721 tgggtgtagg gtgtggggga ggctggaata attaacttcg ccatgttcga ctgcgggttt
    24781 ctactggcaa tcgcaaacgt ggttgcccca agcccgagta agacggccac cgcaatcatt
    24841 acaccgcgca atacattacg tgctggggcc ttggccgccg cagctttctg ttgctgataa
    24901 gtggaaacaa gcaggggttg tgtttttgac tcgagacggg caagggcgat ctttgcttca
    24961 ttattccccg ggttgatttt gagcaccttt cggtaacact caacctgtcg ctctctttcc
    25021 tctactacac gtgcaagcag catccaggcg ggttcatctt cgggatgcag ggtgagcaac
    25081 tcaacgagat atttcttcgc gagttccctg tttccgcgct ggaagttgta atcagctaat
    25141 ttgagaagtt cttgtttgtt catggaaagc cgtagggaca cggcaggccg tgtccctaca
    25201 ttaggtttta tttctagacg ctcggctcgg gttgtttttc cgcggctttg tttaatacca
    25261 cttcgccgtc gtcatttaca tctacaaaaa tagtgtcacc gttgatgaac tcaccggcca
    25321 gcaagcggtc agagagcggg tcttccacgc gctgctgaat gacgcggcgc agagggcgcg
    25381 cgccgaattc agagtcgtag cccatggtgg caagggagga aagcgcttct tcagaagcgg
    25441 tcagaacaag gttgtgttcc accagacggt cggaaacctt tgaaagctca agagaaacga
    25501 tcttctgaat atcttccttg ttgagcgagc ggaagatcac caccgaatcc atgcggttga
    25561 tgaattccgg gcggaaggcg cgcttgagcg actcgttcaa tttcttgcgc atgtcttcgt
    25621 aggacaggcg ttcctcggtg gcttcatcgc gtttcattgc aaagccaagg gaggtttgat
    25681 ttttgatcat gtctgcgccg atgttggagg tcatcacaat gatggcgttg cggaaatcca
    25741 ccttgtggcc tttcgcatcg ctcaagtgac cttcttccat gatctgcagg agcatgttgt
    25801 gtacttcagg gtgggccttt tcgatctcat cgaagacgac gatcgagtac ggcctgcggc
    25861 gcaacgcttc ggtaagctgg cctgcatctt catagccgac atatccggga ggagcaccca
    25921 ccaaacgggc ggcggtgtga cgttccatga attcagacat gtccagctga atggcggcgt
    25981 cttcgctgcc aaacataaat ttggcgagcg atttggtcag ctcggtcttg ccaacaccag
    26041 tggggccaag gaacatgaat gaaccgatcg gacgcttcgg gtctttcaac ccggcacggg
    26101 cgcggcggac ggcgcgggag atggcgatga tcgcatcttc ctgaccgaca atgcctttgc
    26161 ggagttcatc ttccattttg agcaggcgct gtgattcggc ttctgcaagc tgcataagcg
    26221 gcacgcccgt ccacatggag acgacttcag cgatatcttc agcagagacg acagggcttg
    26281 tggtgcggtc ccaaccggtg cgcaggcgtt cgatctgctg gttgagatcg agctcgcgct
    26341 cttcccattc cttgatgtct tcggagttgc cttcttcctg agccaatgtg cggttttggc
    26401 gtgcctgacg caattgcccg aacaggtctt tggcgtgttt ggcggcaggg ctcttgtaca
    26461 tgcgcacacg ggaggaggat tcgtcgatca ggtcaatggc cttatcgggt aaaaatctct
    26521 cggtaacgta gcgtgaagat aaatgtgttg cggcttcaag cgcttcgtcg gaaatgacaa
    26581 gatggtggtg ttcttcgtag gcgctgcgga tgcccttgag gatctgaatg gtctcttcct
    26641 cagagggttc gtccacctgc accgattgga agcggcgctc aagcgcagca tcggattcga
    26701 tgtgcttgcg gtactcgtcc atggtggtgg cgccgatgac ctgcaattcg ccgcgcgaca
    26761 atgcaggctt gaggatgttt gccgcatcga cggaggaacc agcggctccc gccccgacca
    26821 gcatatggac ctcgtcaatg aagaggatcg cgcctgattg ttttagctca tcaataatgc
    26881 gtttgagtct ttcctcgaac tgaccacggt acatggtgcc agccacaagg gatgcaacat
    26941 ccagttgaag aacgcgcttg ttcatcaacg gagcggggac gtcaccatcc acgatgcgtt
    27001 gggcgaggcc ttcaacgatg gcggtcttgc cgacacccgg ttcaccgatg agggcagggt
    27061 tgttcttggt gcggcgcgcc agaatttgaa tgacgcgttc gatttccttt tgacgcccga
    27121 tgacggggtc cagttttttc tcttctgcct tcgaggtgag gtcggaggcg agttggtcaa
    27181 cgagaggggt tttgggggag gcgtttgcgg cgccaccttt gcccggttgc gggccggcag
    27241 gcgtcgggga ggaggaggaa gcggattcgt tcaggacgcg gcgagtttgc ctgcggacct
    27301 ggtctgctgt gacgccgagt cggcgcaggg cttccaatcc agtagattca acccgcacga
    27361 gggcgagcag aatgtgttcc gtgccaatgt agtggtggct gaggcggcgc gcttcatcca
    27421 cagcaaattc gaggacttgc tgggtttccg gcgcgagttc gactcggttg gggtcgaaat
    27481 tgaccgcact gcccgagacg cgttgaacga cttcacgcac acgttcaggg gtcatgccaa
    27541 ggtcgcgcag aacacgtccg gctactccgc cttcttcatc cattaagcct aagagaaggt
    27601 gttcggttcc aatattgttt tggcgagcgc gctcggcttc ttgatgtgcg aggctgagta
    27661 cgcgccttgc tcgctgtgta aatctttcca tgccagccat agttttctcc tatatcctaa
    27721 ggcattgtac caaaaaggcg agggactctg cgtaggaaaa aggttagagt ttgcttacgg
    27781 gttttgagtg caggtagccc ttgcagcctg aaatttggat tatttgataa tcagtcctca
    27841 ttttgggctg gaatcttacc gtcaggatgc ctatacaaat ggctgggttt tgtgtcgggc
    27901 gggagacgcc gccgagagca tctttttaca aaagttattt cgaccgacta cctaccattg
    27961 atgtatcggt ttaagcaggg tatgcctttc aaagtggctg tcaacttccg cttctgcgac
    28021 gagggtgtag atgcgattca tcaattcttc tgtttgtgca tgaatatcag cctgggcatt
    28081 tggattccag ggtgtgccaa tacgcatgta gctttttcct gagaattgcc atgcaccaga
    28141 ccgcggagtt ccctcccatt gaaaattgag gcgggtcaaa ttttgtggtg acgtatataa
    28201 gcccagcggg atgatcggtg cgccggtttc aagcgccatg cgtaccacgc ctgttttggc
    28261 agggatgcgt ttccccaact caacatcctg tccttcagga aagatggcga tcgtaccgcc
    28321 attgcggagg atggcacacg cctgttcttt ggcttccttt gctttgtcgg ttccgcgata
    28381 tacggggatt tgccctgacc ctttcaacat ccagccgatt actgggattc tgaacattcc
    28441 atcctgcaaa aggaagtggg gggtatcttc gaggagaaag ggcaggtaaa gcggatcgaa
    28501 gcccggggta tgattcattg cgatgatctt ggggccgttc ggcatgggga gatttccgtc
    28561 aacatcaaaa ctgcgattaa ggatggtgaa ataggatttc aggaacattc gggaaatttg
    28621 tacgggtgtg aacatgtcat cctccttatg cgtttagaaa ttcaggtttc aggtttttga
    28681 aagccagcca gccaaggcag gtcaatatga cgaggatggc aatgacgacc caggcctgcc
    28741 agcccagttg acttccgaac gcgccaaccg cgctcatgaa gaggcaggcc aatccgcgcc
    28801 cgaggctgtt ggcggccgta aaacggcggg aggtgatatt tccaagaccg gctacgtagc
    28861 acatggcgtc atttgggaag atcggcagaa ccagtgaaag tgtaaaaaaa acaattccct
    28921 gcccggctgc ggccttgtcc catttcgaga ggacatcggg tgcgatccag cgttcggcaa
    28981 atggacgtcc ataatggcgt gcgaggaaga atgcggcctg cccgcctgcg aacagaccga
    29041 tccacgagat caaaagcccg ccccagaaac cgtagatata tccgcaggcc accatgagtg
    29101 cctgccccgg gatgaaggca aggaaaacct gtaaaacaag caatacgatc agaacgaagg
    29161 gtccccaaag tccaagcccc tgaatggagt tgaccaccgc ctcgcgattg ccaaaccagc
    29221 cgagcaaatt cacagcgggc acccgaaatg cccaggcggc gatcaaaact gctgtaacca
    29281 aaatgatggt gaatatcttt ttcatggcga attcctttcg tggctccact ttctcaaatt
    29341 tcaactgcca aagccagaga agaaactgcc aaaagacgcc aacttaaaaa aatcgtccct
    29401 gaaggctcaa accttcaggg acgatgagta attacgggcg gctatttcgc cgttgtcttt
    29461 ttttccatca acagcctcgc cagcccgcgg atggcgttgc ggcgggtacg gttgaaagtg
    29521 ccctcggaaa tatataaccg cgccatgatc tcgcggttgg gaaccccttc cacgtaggca
    29581 tcgtgaagaa cggcgtggtt gtaccaaacg cgcggcaacg gttcaggcgg gcgtttttca
    29641 gacggcttca acaggtcaat ggctgcgacg atggtctgct gcaattccct gccgcgttcg
    29701 atgtgtgacc ctgcacttac tcccagcctg tcggcaagcg gcgattttcc caaagcgatg
    29761 taatctgata aatggcgcaa tccttcttca accattttga tgaaatcctg gtcagggttg
    29821 atttcaattg cgtcgatcat tcctgcggcg gcaagggtcg atccgctttc atcaatttct
    29881 gtctgagtgg gttgaacctg tggagcgttc aacgacacga ttgtgccgat ctggtctgcg
    29941 acctctgaaa gcagatccaa atctccacgg gagtaatcca gcctgtcgtt gggtttgccg
    30001 acccctacaa tggcgatctg gatttgtccg tcgaatgatg gggcgaaata aattatgttt
    30061 gggatctgat cgttttgatc ctggacaata tcttcgtatg aaaccagtga ggatgaaaaa
    30121 cgcgcatcaa tctcgaccga gagcctgctc gctgttacga cgaattcccc tccccgttta
    30181 atggcgatga atccgctgga tgtatccagc gtgctgcata aaaggtcgag gccttcctga
    30241 aggcgcaggt gcaacgtgtc ttcgggagtt tgtccgcttt ttagtttgtg cagttgtttg
    30301 cggagcatct cttctttgcg aatgcgcaga cgttcaaggg attcgcgcac aaggtcgtac
    30361 agggcatgtg tcagcaccac gaatgccgcg agcgggccga ccagctgcaa ggggagtccg
    30421 aagcgcagcg agatcacagc gtagattgat gtgatgacca ggatcgtcag gcttgtgatc
    30481 gggaaatcct gcaaggttgt gcgtctctcc accagggttt gatggtttgc aacagataag
    30541 cccatgagaa aaacgccgct aaagatcaac aggtcttgaa tcaggcgcgg cagcggcggt
    30601 gcgagggcga gggccagtgc gccgtaaagc acactcacgg ctgggaagat ggacgcaacc
    30661 agaaaaaatt ttccctgagc ggtcatgccg atctttctgt gtgagaacag atttttaaaa
    30721 atgccgaaac aggtggctgt ttcaaaaacg gcgtacaaaa tatatggcaa gccgattccc
    30781 atgcgggcga cgtaaatttg gttgccctgt tccccgatga atgaattggg cgtacttagc
    30841 aacaggatga tcgatatcag gccgagggtg taaaggctta ttgtccacgg caggcgtgac
    30901 ttgcggtttt ctacgggtag aagctgcagg gtgatatcgt accaggcacc cagaccgatg
    30961 ctgaggaatg cggcacgcag ggcggctgtg cctgtaattt gattgaaaat attattgtat
    31021 gcaccaaaga aaaaagcgga caaggacagg agcagaatca ctccccgcat ggtcaccctg
    31081 cttggaaacc ctcttgcaaa aaggtagaac gccatccaca ggcatatcgc cattgcggtg
    31141 atgtcaacca ataccgtcgc ttgaaatagc atttcttctc cttgcctgta caaaccaatg
    31201 gagaacagtg ttgccgtgta tcagaaccct gacactttaa cgattatccc catcaccctg
    31261 tatttttccg cgtgcctgcc tgtctaaaag tttggctaaa ttggcttctg ccacttcatc
    31321 aagggaaatt tccagttcgg tgcaggtttg ggcgagatac cagagtacgt cgccaagctc
    31381 ggctttgagt gcttcgcgtg tttcagcgct gatctctccg cttttatcgc ggaatacttt
    31441 tttgatcttg cccgccactt cacccgcttc gttgaccaac cccagagtgg ggtagacaac
    31501 gccgtgccca atggcagggt attttgcagt gacacgtgat ttgctctggt aatcgttgaa
    31561 gttcatgagt tttccttcag tatgtttttt ttggataaat ttctcatgaa ttggatttta
    31621 cattactttt gaatagccat ttcctttgcc tgaatgcaaa atgttgcagg ttgcggaaaa
    31681 aaataaaagc cctgtacctg ttcttcagat agtacaaggc tcttcgtgtt cagagtggtg
    31741 gaattatttc aggctggttt gtattgcggt tatcaagttt agaataaaat tgacaccggc
    31801 agattcggat gggtttgagt aaaactcatt ccaacgttcg agtagctgcc tgtcttttgt
    31861 ctctttgatc agcgtgtcta ttgccagaat gtctgtaggg gtcgcgatag tggagaatgc
    31921 atacaggatt tgcagtttct tgctggcatt ttcgatcttc acattaagtt caaggttttc
    31981 ggcctgcgct ttttctagct tggtttgcgt gttggtcagg tctttactgc tttcttccag
    32041 gtcagccttg acctgggcga gttctgctgt cagattttcg ttttcacttt ggagttttgt
    32101 gtgatctgac tgtaattcag ccaattgctg ttgtgttgcg ataagacttg agtttaatgt
    32161 gtaagcccag taaccaagcc cggccgttgc tgccagaaaa atgatcagaa ataccgtcag
    32221 gataacgggg gttgggtttc tttgtgcggg ctttgtctcc tgaatcagag acattcctgg
    32281 ctgttctgtt tctttttgag aaaatgattc gggttgcatg ctatcctcca tatttgtgca
    32341 atttgattta aattattttt gctggtgctt ttgggatggc caatccctaa ctagaaaata
    32401 ccccggcatg atgaagccat tatactaatt ccatacttaa agtcaactag atattgcgga
    32461 tcttctactt ttaatctatt tggttcactt ccttttcaaa ccacaacgtt ttatgttcca
    32521 gacgaaaccc aaccgcctcg gctgtccgtt gcatggcgat attgtctcca ttagtgccga
    32581 ggctcgcgga tttcatgccg cgttctttga gcagctgcat tcctttaagc aggagcgcct
    32641 ttgccaaccc cagccgttga tagcgcggat gtgtggccac ggggtcggtg tatccctccc
    32701 cgttcgcatt gaccgaacag gtgcaatatg cggcgatgct gccatcgggt gcaataacca
    32761 caaggtcaag cgacgggtcg tattcgcttg aattcatgat gatcaggcgg ttttccgtgg
    32821 tcatgtattc cgtaccaaat gcggatcggt gcgtgctggc aactgcttct gcttcctccg
    32881 tcccttttac agcgcggatg gagaatccgt gcggcagttc aggttcggga atgggttctg
    32941 aaagcaggcg tttcatgccg accgagatca catctgtttg atggaatccg tgcttgcgga
    33001 taaattccag tcgttcgtga ttttcttccc tgcaacttgc atccagtgtg gatgtttccc
    33061 ccgtgttcaa tttcctgcgg cttcgcatgc aggcttcgcc ccatgcaacc atttccacgc
    33121 cgacggagtc ttcgaattgt ttttccactt cccagcgcag gttgttccca tcatccacat
    33181 aagcccaacc gatgggacga tctccttcga accacaaccg tgtattcgca cggatttctt
    33241 cattcgccat gttttcttcg atatccacct tgacggggaa gtcattcatc cgtgcagcgg
    33301 gacgaatctt tgccagcagg ttgagcatgg tttgaaagtc gtttgggccc tcatagagtc
    33361 gactgatgat tttcaagttg gactccttta taaaaacctg gagcgcggac tttagtccgc
    33421 tgtgtggcgg gcagactaaa gtccacgctc tgctaattaa acgccttcgc cagattcctc
    33481 caccaatcat ccttttcacc ggggacaaag ggtgtgtaga gcgtcaggcg atctgcgata
    33541 cccgcgtaac gcttcttcaa atcaacagcc agattttcct gtgtggtcat cagacagaat
    33601 tcattcagca tgtcatcggt gatgagcatg ggcatttcgg cccactcgcc tttggcggca
    33661 aaccccgaaa gtttttcggc ggttgcgccc cagccataaa aatccatcac ggatttataa
    33721 gacggtgtgc tcgcgtagaa agcgatctgc atccgtgcga aactttcttc ctcgggtgat
    33781 gtcgcaataa acggagtcat cgaaacggtg atgtcctgtc gtttgcggcc ttcttttttc
    33841 agaccttctt caatcgcagg cagcatcact tcattcatgt agcgcgaact attgaacggg
    33901 tgcgcgtgga atccctcgca tagttcgccc gccagttttg ccagccctgc attcacgcct
    33961 gcaatataaa ttggaatacc cccacccaac cctcgcccat tttccccgaa aatgggcgag
    34021 ggcaagggag ggggttgaaa aaacggcgac atcaacgtgg ctttgtaata ctcgccgcgg
    34081 aaattcaact tcgttccatt ttgccagcaa tcccaaaaag cgcggatgac ttgaatctgc
    34141 tcgcgcaatt ttcccgtcac cgattcaggc caggccatgc cgaaacgccg ctcaatatgc
    34201 gccttgacct gtgtgcccaa ccccaaaata aaccgtccat ttgactgtgc cgccaaatcc
    34261 catgcggtgt aggcaaggtt ggcgggcgag cgcgaaaacg agaccgcgat ggcggtgcca
    34321 aagcgcagag tctctgaatg ttcagcgatc agcgtacagg gcagaaaagg atcgtgctgc
    34381 gtttcctgag tccacaacgc atcgaatcca attgcttgtg cggcttcggc gatcgcagga
    34441 atatccttca ggccaacaat gggtaatgct gcatcgagtt tcatggtttc cctt
//
LOCUS       DAOQJC010000032        34321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_554_length_34321_cov_8.734285, whole genome shotgun sequence.
ACCESSION   DAOQJC010000032 DAOQJC010000000
VERSION     DAOQJC010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 34321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34321
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_554_length_34321_cov_8.734285"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PLE39_06630"
     CDS             <1..836
                     /locus_tag="PLE39_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNE68037.1"
                     /translation="ADGENIIRIGEDVAQGQVVIPKGTVLRPAEIGGLTALGFTSLRV
                     ARKVMVGLISTGDEVIEPNQTPRPGQVRDINSYTLGALVEKAGGATKRYGIIADQFSA
                     LKEAAAQALSECDVVVITAGSSASTRDMTAEVIRQLGEPGVLVHGINTRPGKPTILGV
                     CNGKAVIGLPGNPVSALVNGYLFVVPVIEKLLGILPKPKASVRAMLTVNLPSQAGRED
                     WWPVKLIAEQPASNVKYKAEPIFGKSNLIFTLAAADGLLRIHPDATGLSAGEVVEIVL
                     I"
     gene            850..>1140
                     /locus_tag="PLE39_06635"
     CDS             850..>1140
                     /locus_tag="PLE39_06635"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE68038.1"
                     /translation="MQNQRKESRKKLIAFTPVYELLHKKLIGYVGDLTLQGVMVIGET
                     SLEAGRRLAIGIEFPADELDEKLQRVAVSGRVAWCRPDEDTPQYLNIGFEFLE"
     assembly_gap    1141..1240
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1372..6132
                     /locus_tag="PLE39_06640"
     CDS             1372..6132
                     /locus_tag="PLE39_06640"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE68039.1"
                     /translation="MNFQYSPVAFVLLITALISIFIAFYIWPQRKTRGVTALIILSLS
                     AAQWLIFYALEVSAIDLETKFFWGKLEYVGIVLAPLSWLLFSLDYAGLERFLGRRAIV
                     VYSIIPAITIALAVTTEYHGLIWSEYHIEYLANFSALGVTHGLWFWVHFSYSYIILLI
                     GTALLIRVFLSRQEGMYRSQGVALLVASLAPWIGNALYLTGNSPIPNLDPTPFAFTIS
                     IAALAWAIFGLHLADITPLARDLVIDSMREGMIVLDARNNVIDINTAASLMIGVSIPD
                     AIGKTAADVFSPWPHLIEKFRSAVDVNQEIAVGTGDSQRRYQVRFSALTGQQDELLGR
                     VIMLRSLDESLVLAPKVQTEKSASRSMLEADTQPQADSPRNIFSLLYNFFFAPAKTDQ
                     KAPANMNPGWFQARERSFTIITRVSALIGSIVFIFVFFFMDDAELQRVNLIYGVAVVL
                     LWSFGLLRTLDFNYRAFLFLFMVYALAVVETINYGFSVESFIFFTAFVVTAVVLIGRS
                     GGWMAAAISLFSLFIFNWLIGTRYFIPVNLTLETVVPPDFGSGLTSLLVFSASSLAMV
                     SSITILMESLNRAWQSELQSSNLLQQERDLLEQRVDERTQDLEDARDSAVRSNIELRK
                     YYRVIEQSGNSIVITDLDGNIQYANPRFEEVSGYALEEVLGKNPRILKSGRQKPEFYE
                     KMWRTISSGHIWTGVFHNRRKDGTLYWESATIAPVLDQDNVVVNYVAIKEDITSRREV
                     ESQLRKLSRAVEQSGNTVIIMDRNGLIEYVNPKFTEVTGYSPEEAAGKSPIALMQQVE
                     EIPDFREDDWWLTVNNGQIWQGEFRNHRKDGSVFWESASIAPVHGRDGEITNFIEIKQ
                     DVTEQKILQEQLQKQNDYLSILHQVTLDLLNRRDLTDLLKTIVNHSALLLDAPLSELM
                     LIEGDKLVVKAVTDNQLDLLEERVGRDQASLSWKAFDTLQPVLLEDYSTWSGHRPKYD
                     DFQLHAVADFPVVAGDHCFGVLALGRNKPDYPFTPEQTQTGILFARLVALVLDNVNLY
                     DSAMQEIMERERVQASLQRSNQQQQVINSLLKVSLENRSMDEVLGSILDEILSIDWLT
                     ISPKGGVFLFNKQTNLLELRTHRNLSPHLQSLCAGVALGHCLCGRAAQTRQIQFADCL
                     DERHETRYEGIEEHGHYNIPIVDGEDILGVIVLYLPHGYQKSENDLVFLNSVADAVKG
                     IMQRKQAETLLLESEIRFRQIVENASDIIYRADINGNFTYANPSALKMMGFMSEKDVI
                     GKNYLDLTTSEYRQKLKRVYDHQYISKTMSTYHEFPAVAADGGIVWVGQNVQLIMDGD
                     KIIGFQAVARNITQLKQAQEALALSRDQALEASRFKSQLVSRVSHELRTPLGGILGYA
                     ELLQVEAFGGLNEKQQAALKNIVESTNFLTTTVNDLLDQAQIESKSLSLHNAYFSPAT
                     LLSKISAPMSVLAEKKGLKFRTEISPSLPLELYGDEKRLQQVIINLAGNAIKFTSAGE
                     VYINVSSPIPAYWAIEVRDTGVGIPENEQANIFEPFRQVSNSITRENRGSGLGLAIVR
                     QLVELMGGQISLQSESGKGSIFTVTLPLINAPGE"
     gene            6135..6491
                     /locus_tag="PLE39_06645"
     CDS             6135..6491
                     /locus_tag="PLE39_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE68040.1"
                     /translation="MPIQPYAIVVEDDPKLGAIFQTTLQHAGFETALDHNGDKYQAFL
                     NIAEPALVILDLHLPFAFGGDILEVIRARYPNTVVAIVTADLYKAKAFNGKADHILIK
                     PVSVASLLKLAESVKG"
     gene            6501..6863
                     /locus_tag="PLE39_06650"
     CDS             6501..6863
                     /locus_tag="PLE39_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE68041.1"
                     /translation="MPKPLALVIEDDPQLNQIFTLTLFESFEVETISDGSQALDRLTE
                     IIPNVVVLDINLPGVPGDQILRMIRSNSRLHSVKVILATASTHQARDLQDMADIVLLK
                     PISPMQLRELAERLSRMG"
     gene            complement(6900..7403)
                     /locus_tag="PLE39_06655"
     CDS             complement(6900..7403)
                     /locus_tag="PLE39_06655"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HNE68042.1"
                     /translation="MKNSCSSPLHPQAIEGLRLFNQRKYFEAHEALEDAWREETGPVR
                     ELYRGILQVAVAYLHITRGNYEGAVKVYGRSLKWLKDWPDICRGIGVEQFRKDAERVM
                     NQVVILGKDGIRNFNPSLFHEITWNEQRVWICDRCGTEMYEKNCKVSCPNCGNRFDCS
                     DLNIYFD"
     gene            7462..8355
                     /locus_tag="PLE39_06660"
     CDS             7462..8355
                     /locus_tag="PLE39_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HNE68043.1"
                     /translation="MRKQLEDRRWGIALAFFALISLVLLAGALQNANFLPAQPLGGSE
                     SDQITIDLSVLKDALVNSGEIPLWKQFVFFGAIFLFLSLLALFLSPELRKKLFLMLLR
                     LLASVIMVYYLLKVKPDIFSGLLQFGQLGQSDGQIQDVPAPVFQPPQISSATTFLLTL
                     AFVVLVAFLFWLINRWWTRQKELIAKRRPLAEIAAIARASLHDLASASNSNDAIIQCY
                     VRMSNVVAMKRGLQRQYAMTPAEFAAYLEGAGIPPEPISRLTGLFEAVRYGGHISGPR
                     EIDEASDCLKSILRYCGEAVS"
     gene            8352..8960
                     /locus_tag="PLE39_06665"
     CDS             8352..8960
                     /locus_tag="PLE39_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68044.1"
                     /translation="MKRRYLLLALLMVGAVVVSYLLKEVIYQTVFVPLAYLWWLFGLY
                     YRAVPQLILWILLVVMVFISSLKLVPIINFFPAVEKKIKNETVGPVEQLSMMLNKPSR
                     GVYYKWMIANRLGRVAREMLDQREGRTFKGYSRLRGRDWTPPADVDEYLETGLNGSFA
                     DYPTPHWWREAEPTPLDLDPQKAIDYLENEMEMNRDRNRKSI"
     gene            8935..9888
                     /locus_tag="PLE39_06670"
     CDS             8935..9888
                     /locus_tag="PLE39_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNE68045.1"
                     /translation="MTEIVKASELSKQVISEVERAIVGKRSLLEMIMAAVLAGGHVLL
                     EDYPGLGKTLIARSFAAALGLDFKRIQFTPDLLPGDITGGYIFNRTKNKFELRQGPVF
                     TNILLADEINRASPKTQSALLEAMQEGQVTLEGESMKLPDPFIVLATQNPIEYEGTFP
                     LPEAQLDRFMLKLTVGYPDLEEEREILKRRRERKQDELALKQVIKPKQLLEMRAALES
                     VHVDSDLEGYISALVHATRTDRRVSVGASPRGSLAFLKMARASAVLAGRDFILPDDIK
                     RFAVPVLSHRLLLQPEYWMSQQVNGDVIRDVLSHTPVPVIK"
     gene            9890..11170
                     /locus_tag="PLE39_06675"
     CDS             9890..11170
                     /locus_tag="PLE39_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNE68046.1"
                     /translation="MYRYVLLSALVYGLLLAGLATLQGTFVALAIPFMLFLVYGFLNA
                     PEELNIQIQRTLSTDRASADADVVVTLKVTNNGAAIEELLLDDRISPNLTLRNGSPRH
                     LLRLAKDSTCTLTYSVAGTRGMYHFDQIHLTVTEPLGLIRREQTVHAEKRLTIFPLVT
                     KFKSVAIHPLRTRVYAGTIPGRIGGPGTEFFGVREYQMGDSPRTINWRASARHPGNLF
                     SNEYQQERVADVGVVLDARASANIFHVDQSLFEYSVAAAAGLADAFLAQGNRVGLLVY
                     GSYLGWTMPGYGKIQRERIMRALAGAEVGVSSVFSGLQHLSPHMFPPESQIVLVSPLV
                     NDDLEVLIQLRGRGYQVMVISPDPARFESRLLADHPKVDLAERIVRMERSLLLRRLER
                     AGIQVIGWDVSIPLDQAIGPFLTRPHRSVFRRSS"
     gene            11167..11655
                     /locus_tag="PLE39_06680"
     CDS             11167..11655
                     /locus_tag="PLE39_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68047.1"
                     /translation="MTEFVLVASIVLSAGSLAWVFFEAGLSDFGRWVLIFGVAWLVAQ
                     RVGWKWISSVGLVLAVLLAATGLWFGLTPGWMFAGAIFALAAWDLTDFRDRLRFVAVD
                     DNARGMERRHLARISWLVLVGMALASITMVLQVKFTIEWGALLVVVILIGLGQLVNWL
                     RK"
     gene            complement(11682..12230)
                     /locus_tag="PLE39_06685"
     CDS             complement(11682..12230)
                     /locus_tag="PLE39_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68048.1"
                     /translation="MKPFPYRKVVIVGTTSSGKSTLAKALAEKIGAICIDLDYLHWEP
                     NWVEAPNDVFRERVEKAISAESWVVAGNYSATRDLIWPQAEAIIWLNYSFPIVFWRLF
                     TRTFRRVATKEKLFSDNVENFWVQIKFWSQDSLFNWLFKTYWRRKREYPNLFSQPENA
                     HLTIIQFEHPQEAEKWLAAISA"
     gene            12328..13677
                     /locus_tag="PLE39_06690"
     CDS             12328..13677
                     /locus_tag="PLE39_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE68049.1"
                     /translation="MFELPDDEILNLSKEHVFWTWSAQAKVNPIAVKKAKGVYFWDVD
                     DKKYLDFNSMTMCVNIGHGNERVIKAMQEQAAELPYAAPGMATKIRALASKAVAEITP
                     KGALSKVLFTLGGADANENAIKLARGYTKRHKILTRYRSYHGASAGAMAATGDPRRVA
                     WEPNLMTGVVHFLDPYRYRSTFHRTNPNISEEEFTRDYLNHLEEIILYEGPESIAAIL
                     MESVTGTNGIIIPPNGYMQGVRALCDKYGIVMICDEVMSGFGRTGKMFAIEHWDVVPD
                     IMTMAKGLTSAYAPLGAVAMKPEIAAAFNEHAFESGLTYTSHPISLAAAVANIEVMKE
                     ERIVENAAAMGGTLRRMLTDLGEAHPSVGEVRNIGLFGILELVKDRSTKEPMAPWNGS
                     SPEMNALKKYCTDHGLFLYTHWHTVLIIPPLIITEEELKEGMDVLDKALEITDKVVK"
     gene            complement(13695..14675)
                     /locus_tag="PLE39_06695"
     CDS             complement(13695..14675)
                     /locus_tag="PLE39_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE68050.1"
                     /translation="MLFIAGTAAILYWAFSEYAGVRNIWLLLCCTPVAVIMLGVFAVF
                     SMYMRFGKPLSQIFNAIDSVAEGDLSVRVPENRSDMFSELITRFNKMIAELERADQQR
                     RNLTSDIAHELRTPLHIIQGNLEGIIDGVYEPTTEHINNTLDETKLLARLVNDLQTLS
                     LAESGQLPLHPTRFLLADLTHDLTASFSAQAATLGIDLTTELANPTQEITADYDRLNQ
                     VLSNLISNALRHTPKGGTISLETESGLSEAGSVRIKVKDSGEGIASEDLPFIFDRFWR
                     GDKSRTGRVHSGLGLAIAKQIVHAHNGTIEVESEIGRGTTFTIEIPTGHV"
     gene            complement(14795..15472)
                     /locus_tag="PLE39_06700"
     CDS             complement(14795..15472)
                     /locus_tag="PLE39_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE68051.1"
                     /translation="MSEIILVVDDEPKITRLARDYLEKNGYHVLTASDGQSALATARR
                     EKPDLIVLDLMLPNMDGREVCKILRRESDVPIIMLTALAEEIDQVTGLEIGADDYITK
                     PFSPRALVARVRALLRRSKGGIKTPTTIRIGGLEIDSEKYSVTLNGTPIKLTPNEFKL
                     LSILASRPGQTLTREQLLEDLHGAASSIDRSVDSHIKNLRKKLEAESDSPSIETIYGI
                     GYRFIER"
     gene            complement(15529..16335)
                     /locus_tag="PLE39_06705"
     CDS             complement(15529..16335)
                     /locus_tag="PLE39_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE68052.1"
                     /translation="MNRNDILIEADELLKKLGDKNIRIFDATITDDAYLQEHIPGAAF
                     LEHENFSAPESANRYTTMLPEDKLAEQIGALGISNETEVVVYACGMLPYAVRAWWVLR
                     YAGHDKVRILNGGLSAWKQAGGQTEQEIHSYEPANFQPSLRKWMYADKEEVLKSLKET
                     DIAVVNVMPRESYDARHIKGSICLSSMNLMQQLDAFLPDDQLAAHLQEVTHNKRVITY
                     CGGGIAATVNAVAHLMTGYEDVTVYHGSMFEWLAEDLPTVGDGKWEIWKK"
     gene            complement(16517..17005)
                     /locus_tag="PLE39_06710"
     CDS             complement(16517..17005)
                     /locus_tag="PLE39_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68053.1"
                     /translation="MKKGPFILFRVIGFLLVLGLIAGAGAFAYKAGMAQGISQAPAVA
                     TAIAQAAEDGQAVPPMVYGHGFAPYGYGYGMHGHGHFGFFPLGGICFGIFLLFFFFGF
                     MKMMFFRRMRHGWGHHGGHGPWGKNWEGGVPPMFNEWHKRAHGETPAADGEKPAEEKK
                     SE"
     gene            17281..18219
                     /gene="trxB"
                     /locus_tag="PLE39_06715"
     CDS             17281..18219
                     /gene="trxB"
                     /locus_tag="PLE39_06715"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNE68054.1"
                     /translation="MTEKHVKVLVLGSGPAGLSAALYAARAELEPIVLTGMQLGGQAA
                     LTHLIENYPGFPEGVGGAQLGELFQKQAEHFGAKVEFNMAHEVDLSQRPFTVKTDDGD
                     YKAETLIVTTGANPNHLEIPGEVELTGRGVSYCATCDGWFFKDKKVVIVGGGDSAFEE
                     ALFITRYASSVTLIHRREDFRAGAILQKRAKEHPKMNFILNTVVTEVLGSDKLEALKL
                     KNVQTGEESIFETDGLFIFIGHTPNSQMFKGQLEMSDLGYVLVNDKMEASVEGVYAAG
                     EIADPHFRQVITSAGMGAAAAIQATKFLEEKEAVHS"
     gene            18443..19378
                     /gene="mdh"
                     /locus_tag="PLE39_06720"
     CDS             18443..19378
                     /gene="mdh"
                     /locus_tag="PLE39_06720"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNE68055.1"
                     /translation="MKKISIIGAGNTGATAAHWLAERELADVVLLDVVEGMPQGKSLD
                     MLEAMPIIGKDTHVVGTNDYADTKNSDIIIITAGIARKPGMSRDDLLKTNADIVGKAA
                     METLKHSPNAIFIVLTNPLDTMAYLTMKMTKLPRERVIGQAGILDSARMRAFVAMETG
                     VSVENINCYVLGGHGDEMVPLTRHSNIAGIPLKEYLPADKLEAIVNRTRKGGGEIVNL
                     LKTGSAYYAPSMACVQMAEAILKDKKLIVPCATMMQGEYGLNDMYFGVPVVLGAGGME
                     RVVEYKFDAEEKAMFEKSAASVKETHEALKGLVTF"
     gene            19426..20745
                     /locus_tag="PLE39_06725"
     CDS             19426..20745
                     /locus_tag="PLE39_06725"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HNE68056.1"
                     /translation="MILHEKLSAQLPAWRERIKSLAKEHAEVVVDHVNIGQIVGGMRD
                     IKSLLSDVSFVDPAHGIIFRGLSIPELLKALPKARASKMPLVGGLYYLLMIGEVPTKE
                     QALEVEAEWAKRATVPDYVYKMLKTMPKETHPMTLFSQAVLALQNGSNFAAKYHSMKK
                     DVYWEAALEDSLDLTAKLPVIAAYIYRMKYYGETSKPKYNPKLDYGANFSKMMKVSDK
                     KGYSELARLYFILHSDHEAGNVSAHTMHLVGSALSDPYLSFSASLNGLAGPLHGLANQ
                     ECLGWLLEVHKKFGGVPSREDLYKFAWDTLNGGHVIPGYGHAVLRVPDPRFMAQMEFA
                     KKRFPDDELVRLADLVFDVVPAVLKEQGKAKNPAPNVDAISGTLQYYYGVREFDFYTV
                     LFGVGRALGVTANYVWARALGMPIERPKSLTTKMLEDVVAKAAQQPA"
     gene            complement(20816..22873)
                     /locus_tag="PLE39_06730"
     CDS             complement(20816..22873)
                     /locus_tag="PLE39_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNE68057.1"
                     /translation="MPPIAPKRDHALTQHGQTRNDEYYWMRNREDPEVKAYLQAENDY
                     LEETLQHLNPLREKLFHEMKARIREVDHSVPEKHGDFLYYTRTEAEKQYPIFCRKKNI
                     PNAAEEILLDQNALAEGHEFCSVSAFSVSPDQNKLAYSVDLEGSEVYTLHVKDLISGT
                     MYPEAIANIHGSVYFQAGVEWANDNETVFYLSLDESHRACKLYRHKVGTAPSQDVLVY
                     FEEDDTFSLSLFKSRSEKYIFTYHYNTISQEMRFLPADHPVGALNVLQPRKAGLEYYA
                     AHLDDHFYIVTNEDAHNFKLMKTPTAAPSRENWQEVIGHREDTLLEHVDVFAEHIVLH
                     ERKGGLKQLRVSRPDGHSDVRYVNFPDPTYEVIVDVNPEFHTNLLRLRYSSLTTPFST
                     VDFHMDNGEWEVKKEYQIPGGYDKSQYVTEYIHATAADGRKIPISLAYKKGLKKDGQN
                     PTLLYGYGAYGASSDAAFNLSLLSLFDRGFVFAIAHVRGGSEMGRFWYDEGRMFNKKN
                     SFTDFIACAEHLIAEGFTSPEKLASTGGSAGGLLVGASMVMRPELFEAVICKVPFVDV
                     ITSMSDPSIPLTTLEYDQWGNPANRDEYDYMLSYSPYDNLREVEYPHLLLSTGFNDPR
                     VAYWEPAKFLARLRDKKNDDNLALLQTNFSAGHAGASGRYDYLKENVLDFAFLIDRLG
                     AKP"
     gene            complement(22890..24410)
                     /locus_tag="PLE39_06735"
     CDS             complement(22890..24410)
                     /locus_tag="PLE39_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68058.1"
                     /translation="MEKHSPLKIILITSAVFYLIFIIGTSFDIKGERYFTLVDDAMIS
                     MRYARNLAQGHGLVWNIGEAPVEGFTNLGWTLYLAFLHLFPFPASKISLVVMLTSLAI
                     LLANSYTVSKIAEALQPDSKYAPTLAALITAFYFPLVFWSLRGMEVGLLALLVSLAVL
                     FSITEKHPVFIGLIFAAAILVRVDAIISIAPIALYLLLKNKRNAILPIASAAITLLAV
                     FYFQHNYFGDFLPATYYQKMTGFTALERIRHGLLVFVEFAARDTLPLFLFSLAGIFFY
                     KLQRSRETLLLLGIFLAQSAYSIYVGGDYAEPETNAANRFITQGMPALIILFSWMTNR
                     ILTDLQAAQPQSAFTAPKVNPAIPLAVITLLIVSGEPWFTYTVDNAPLLKADIRRVRL
                     GLFLAESTSPEAVIAVHAAGQIPYYSQRTTIDLLGLNDPIVAKGQGHGEFYPGHNKWD
                     YDYSIIHLRPDLIADNFAPLADFMRGKEEYQKLENGIYIRIDSNLINLEKISQDYR"
     gene            complement(24503..25471)
                     /locus_tag="PLE39_06740"
     CDS             complement(24503..25471)
                     /locus_tag="PLE39_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HNE68059.1"
                     /translation="MALLASLFFGFVPMFLFAAFVYWLDRYEKEPKLLLGAAFFWGVV
                     IAAGGSYILNTAFGIGIFMFTGSEGAADFGTTSIVAPIIEEFLKGLAVAIVFFMFRKE
                     FDTILDGIIYGGIVALGFAATENTLYIYRNGYQENGWGGLIVLVFIRVILVGWQHPFY
                     TSFTGIGFAFSRTQRNPLIKFIAPFLGYGVAVTTHAFHNTFGGLIGGFEGLFAGTFVD
                     WIGWGLMLGFIIWMINNERNIVKRQLYDEVTNGIISAAHYQRALSPWTLTMAGLSGRA
                     TSRFYQACGELAHKKEQLARHGDESGNVAIINSLRTELTALAPQVR"
     gene            complement(25483..26103)
                     /locus_tag="PLE39_06745"
     CDS             complement(25483..26103)
                     /locus_tag="PLE39_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNE68060.1"
                     /translation="MRVRITFTKQGALRYTGHLDLHRLWERAMRRADLPITYSQGFHP
                     QPRMSLAAALPLGFSSRCEMLDVRLNDEIPLEDIRVRLKDNLPHDIQVTNIESVDERA
                     PALQTQVVSAQYHVQLTEPVDGSDLKRRVAELLESESIPRERRGKFYDLRPLIEALTA
                     DIESSTLQMTLAAREGATGRPDEVLTALGIEPEYARVERTRLIFQE"
     gene            complement(26106..26630)
                     /locus_tag="PLE39_06750"
     CDS             complement(26106..26630)
                     /locus_tag="PLE39_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68061.1"
                     /translation="MNKSLFLPRLQRERDKFELLLNQVGFSRRMTMKGVVDGLSVKDM
                     LADILSREQFIADRLNEILQGEVYSPCASHSALDAFQSKFGYPDYESPLLNQEARNHL
                     VIHRHKNIGLDDIVEQELAAYENILSAFEHLTHNQCLDHDLFHRTAEHTYKVYRRASL
                     EIQTWLKSIATESN"
     gene            complement(26632..28578)
                     /locus_tag="PLE39_06755"
     CDS             complement(26632..28578)
                     /locus_tag="PLE39_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HNE68062.1"
                     /translation="MMNPEQIELKLDRILLKVQKPGRYVGGELNSIVKDWEKAKTKVA
                     FVFPDIYDIGISNVGIKILYDQINQREDALAERAYAPWTDMEELMREHGIPLYTLESK
                     QPLACFDLIGFTLPYETLYTNTLNILDLAGIPVRSADRNESHPIIIAGGHSTTNPEPM
                     HAFIDAFVIGEGEEVIHDVVDTIQKFKSSPSYKRSELLVALAQISGVYVPKFYETTYL
                     EDGTVAHTLPTIPEAPKLVNKRIVAKLLPPPVKFIVPNIEVIHNRVSVEIMRGCTRGC
                     RFCQAGMITRPVRERSVQEVVDAADAAVRSTGFEELALMSLSSSDYTHISELVDAISK
                     RFEGRKLSVNLPSLRIESVSVDLMDKLKQHRSAGFTLAPEAASERMRRIINKFIPDED
                     IINTTREIYSRGWTTIKLYFMIGHPSETVEDVQAIVDLCRRVIEEGRKVAGWKVKLHA
                     GVSTFVPKPQTPFQWVSCDTKENILEKQALLKRQLLKDKSIRLSWTKPEDSLLEAWLS
                     RGDRRMAEVIYTAWQNGAKFDAWDEAKKQEAWLPAFEAHGLDPAFYTHRQRRTDEVFP
                     WEHITAAVRKNFLFQDFRQSLEGQIRVDCRLNCFACGILPTFANLRRENPGEGWKCPE
                     VKSPVRVTSNQLSVISEQSPLAGD"
     gene            complement(28665..29984)
                     /locus_tag="PLE39_06760"
     CDS             complement(28665..29984)
                     /locus_tag="PLE39_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68063.1"
                     /translation="MKTIHKLFSIFSLIAILALAFATPAQAFDGRTGNKVVIEKDEVI
                     EDDVYVNAETFILEGTVKGDLVVFGTNITINGVVEGDLIAAGQSVAINGSVSDDARIA
                     GAALLISDSAVIGGDVVGGSASFETKEGSSIEGELVIGAAQALLAGNVTGDALLGLGA
                     LDLRGEFGGNVLANVGEPTEEAGPPFSMFMPQVPFSMPTVNPGITIAEGAKINGNFDY
                     TQFADIDIPTDVVGGKVTRTEPVIDPEETITPPTQSELIVDWSLNLLRTIVTLLILGL
                     LLGWLAPTFIKTLVEKVQTQAAISLGWGVVAYAVFFFAIFIVVVAMVIGGILFGALQL
                     GSLSGTIIVVGLLIIFALIVGFVLATAFLTKIVISWLGGKLILARINPALAESKVWPL
                     ALGVIIVALLTALPGIGWLANLLVVLLGLGALWLWGRETMQARKTAA"
     gene            complement(30053..30745)
                     /locus_tag="PLE39_06765"
     CDS             complement(30053..30745)
                     /locus_tag="PLE39_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE68064.1"
                     /translation="MNKEFFDVIKRGYLEVVQRLLSSNPDLIYEKENGLSPVMVALYH
                     REPKIVNYLAEKTGSLNIFEASALGRTTQVVRHLARDPMLVNAYTDDGFQPLGLACFF
                     GHAETAEYLIKAGASINSPSRNQLRATPIQSAAAAGHVEIVLLLLKNRADPNVREQGG
                     YTPLHAAAQNGDVAIIRSLLFNGADLTIRSREGKLPIDMAVESGHVDAAKLLKEGITR
                     RFRTKITLQRTE"
     gene            complement(30859..31974)
                     /locus_tag="PLE39_06770"
     CDS             complement(30859..31974)
                     /locus_tag="PLE39_06770"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNE68065.1"
                     /translation="MKTFKAFLAFTLLFLTGCGTTEMEVPMPSESNSTTPNQQVDEFF
                     AEFVSDNTPGGAVIVVQNGKILHHAAYGLANLEEGTPLTTDHIFHLASVGKQMTALGI
                     LMLVEDGSLNLDDPVGIHVPEVAHFGNEFTIRHLLHHTSGLPDYDDQITDALYTRSDT
                     PTNEDLVAALSEMTELPSTPGDSFEYSNPGYDLLAVVIERVSGENFPDFMQSHIFDPA
                     GMTHTFSLPNEKRRADPLVAMSYTNESGTPEAYPYDDLDNLYGSGSIYSTTTDMALYD
                     EALYTEKFVSQALLAEAFLPTELNDGSIEPYGFGWELEEWNGENYVAHSGAWLGFNSD
                     YIRFPERHLAVIVLLNRDYDYPDDPRIALKIAEYFLQ"
     gene            complement(31971..32507)
                     /locus_tag="PLE39_06775"
     CDS             complement(31971..32507)
                     /locus_tag="PLE39_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE68066.1"
                     /translation="MTEESLDIVNDNDIVTGQAPRSTIHQNGLQHRGAHVFLFTPEGK
                     MLIQKRSADRTSAPSLLDCSVSEHVKAGESYLEAARRGLREEMGITVEELTPLVKFKM
                     IYGPNDNEISTLYKGLVDPASVKFDPVEIAEIRYLSMDELRSVLENEKQTLCGWFVEI
                     LHWYWGHPAAMTILKAWS"
     gene            complement(32631..33389)
                     /locus_tag="PLE39_06780"
     CDS             complement(32631..33389)
                     /locus_tag="PLE39_06780"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNE68067.1"
                     /translation="MSKIKAVFFDQDGVIIDTERDGHRVSFNMTFKEFGFTDEWSVEY
                     YHELLQIAGGKERMKHHWKTKGFSKPLTEEEIDNLVKEMHKRKTALFVELIESGKLPL
                     RPGIHRFMKELMEAGIKIGVCTTSNEQAAKAITEGILSDIKFEVVLAGDVVEKKKPDP
                     EIYNLGLAKLELKPEEAFVVEDSKNGVKAAKAAGLKTIVTTNPYTEKEDVEAGDVIVS
                     CLGDPDGEKAKMRKGDIKDFDGIVHAKQLIELFG"
     gene            complement(33474..>34321)
                     /locus_tag="PLE39_06785"
     CDS             complement(33474..>34321)
                     /locus_tag="PLE39_06785"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNE68068.1"
                     /translation="GNGSSEVYDNIFRMFIEPGDEVIQHTPCFGIYGLRGTLLGAKMV
                     SVPMIYEDHKLKFDPDGLMKAVTDKTKIIVIPNPNNPSGNFMDPEHFEPFAKLGIPLV
                     VDEAYIEYAGLGKSQVELTKKYKNVFITRTLSKAYGLAGMRFGYTIAHEDTLKQVAGS
                     LLPWNVGTIPMWAALAAFEDTEGLAERVKFNNDAVDYISESLSVIPGFYVMQSKANYI
                     LFDCGETGKTGKQVLEYAETKGIILRGESKKYGSDGWFRVTIGSKEENELFVNTVLEF
                     FGMKK"
BASE COUNT         7737 a         8190 c         9564 g         8730 t
ORIGIN      
        1 tagcagatgg agaaaacatc atccgcattg gcgaggatgt ggcacaggga caggttgtta
       61 ttccgaaggg gacggttttg cgcccagccg aaattggcgg attgaccgct ttgggattta
      121 ccagcctgcg cgtagccaga aaggtgatgg ttgggctgat ttcaactggt gatgaggtca
      181 tcgagccaaa tcaaactccg cggcctgggc aggtgcggga tattaattcg tacacgctgg
      241 gggctttggt ggaaaaagct ggcggcgcaa ccaaaagata tgggattatc gccgaccagt
      301 tttcagcgtt gaaagaagcg gcggcgcagg ccctatccga atgtgatgtg gtcgtcatca
      361 ccgcaggttc ctccgcctcc accagagata tgaccgccga ggttatccga cagctgggag
      421 agcctggtgt gctggtgcat gggatcaaca cccgccccgg caaaccgacg attttggggg
      481 tgtgtaatgg caaagccgtg atcggcctgc cgggcaatcc tgtcagcgcg ctggtgaatg
      541 gatatttgtt cgtggtgcct gtcatcgaaa agttgttggg gatactgcca aagccgaagg
      601 cgagtgtgcg ggcgatgctc actgttaatc ttccttcaca ggcgggccgt gaagattggt
      661 ggcccgtgaa gttgatcgcg gagcagccag cctcgaatgt caaatacaaa gctgaaccca
      721 tctttggaaa atcgaacctg atctttactc ttgccgcggc agatgggctt ttgcgcatcc
      781 acccagatgc aacgggtttg agtgcgggtg aagttgtgga aattgttctg atataaaagg
      841 gaggtcgtcg tgcaaaatca acgcaaggaa tcgcgcaaga agttgattgc ttttacgccc
      901 gtgtatgaac tgcttcataa aaaattgatc gggtatgttg gtgacttaac ccttcaaggt
      961 gtgatggtaa tcggggaaac ctcgcttgag gcaggcaggc gtcttgccat tggcatcgaa
     1021 tttcctgcag atgaattgga tgagaaattg cagcgtgtgg ccgtttctgg tcgtgtagcc
     1081 tggtgccggc ctgatgaaga taccccccaa taccttaata tcggttttga atttcttgag
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcaaaccccc aatttcactt
     1261 gtcttttcaa tgacattaca aaaggcgggc ggttttctgt aatgccaccc cttcgcgtat
     1321 aatgcgggaa ttccttgcga aaatacagct tgtaaatttg aagggttgtg tatgaatttt
     1381 cagtattctc ccgtcgcctt tgtgttgctg attacggctc ttatctcgat ttttatcgcc
     1441 ttttacattt ggccgcagcg taaaacccgt ggtgtgacag ccctgatcat cctgtcgtta
     1501 agtgcggctc aatggctcat tttttacgca cttgaagttt ctgcaataga ccttgaaaca
     1561 aaattctttt gggggaaact ggagtacgtt ggcatcgttc tggcccccct ttcctggctt
     1621 ttgttttcgc ttgattatgc gggtctggag cgttttcttg gccgcagggc catcgtcgtt
     1681 tattcgatca tcccagccat cacaattgca ttggcggtca ctaccgaata tcatggcctg
     1741 atctggtccg agtatcacat cgaatatctg gcaaactttt cagcactggg ggtgacacac
     1801 ggcctttggt tctgggtgca tttttcctat tcttatatca tcctcctgat cggtacggct
     1861 ttgctgatcc gcgtgttttt gagcagacag gaaggcatgt atcgaagtca gggagttgca
     1921 ttattggtgg ccagcctggc accgtggatc gggaatgccc tttaccttac aggcaacagc
     1981 cccattccca atctcgaccc tacgccgttt gcattcacca tcagcattgc cgcccttgct
     2041 tgggcgattt ttggtcttca tctggcagat attacgcccc tggcgcgcga tcttgtgatc
     2101 gactccatgc gcgaggggat gatcgtcctt gatgcacgca acaatgtgat cgatattaat
     2161 accgctgcct ccctgatgat cggcgtgagc attccagatg caattggaaa gacagccgca
     2221 gatgtattct ctccatggcc gcatctgatc gaaaaattca ggagcgcagt ggatgttaat
     2281 caggagatcg ctgtcggcac gggcgactcg cagcgtcgtt atcaagtgcg attctcggcc
     2341 ctgacggggc aacaggatga actacttggc cgtgtcatca tgttgcgttc tttggatgaa
     2401 tctctagtcc tcgcgccgaa ggtacagacg gaaaaatccg catctcgctc catgctggaa
     2461 gcggataccc aaccccaggc agatagcccc cgcaacatct tcagcctgct ctataatttt
     2521 ttctttgccc ccgcaaaaac tgaccagaaa gcgcctgcca atatgaaccc gggctggttc
     2581 caggcccgcg agcgttcctt taccatcatt accagagtct ccgctttgat tggcagtatc
     2641 gtattcatct ttgtattctt ctttatggat gatgcggagt tgcagcgggt gaatttgatc
     2701 tatggggtgg ccgttgtact gctttggtcg tttggccttc ttcgtacact ggatttcaat
     2761 tacagggcgt ttttgttcct gttcatggtg tatgcgcttg ctgttgtgga aaccataaat
     2821 tacggctttt ctgtggaaag cttcatcttt tttacagcct tcgtggtgac cgcagtggtg
     2881 ttgatcggcc gtagcggcgg atggatggcg gccgcgatca gccttttttc gcttttcatt
     2941 tttaactggc tgattggaac aaggtatttt attcctgtca atttaacgct tgaaaccgtc
     3001 gtgcctcctg attttggctc ggggttgacg agtttgctgg ttttttctgc aagttcactt
     3061 gcaatggtct cctcgatcac catcctcatg gaaagtttga atcgagcctg gcaatccgag
     3121 ttgcagtctt ccaatctcct tcaacaggaa cgtgacctgc tcgaacaacg cgtggatgaa
     3181 cgtacgcaag acctcgaaga tgcacgtgat agcgccgttc gcagcaatat tgaattgcgc
     3241 aagtattacc gtgttattga acagagcggc aattccattg tcatcacaga tctcgatggc
     3301 aatattcaat atgccaaccc caggttcgaa gaggtttccg ggtacgcttt ggaggaggtg
     3361 ctgggaaaga atccacggat tttgaaatcg ggcaggcaaa agccagagtt ttacgaaaag
     3421 atgtggcgca cgatcagttc cgggcatatc tggaccgggg tcttccataa tcgccgtaaa
     3481 gacggaacct tgtactggga atccgcgacg attgctcctg tgcttgatca ggataacgtg
     3541 gttgtcaact atgttgccat caaggaagat atcacttcac gacgcgaggt ggaaagccaa
     3601 ctcagaaaac tttcccgtgc ggttgagcag agtggtaaca cggttattat catggaccgc
     3661 aacggcctga tcgagtatgt caatcccaaa ttcaccgagg tgacaggata ctcgcctgaa
     3721 gaagccgctg gcaaatctcc aattgccttg atgcagcagg tggaggagat acccgatttt
     3781 cgcgaggatg attggtggct tactgtaaac aatggccaga tctggcaggg tgaattccga
     3841 aatcatcgca aagatggctc ggtgttttgg gaatccgctt cgatcgcccc ggtccatggc
     3901 cgtgatgggg agatcacgaa ttttatcgag atcaagcagg atgtgacgga acaaaagatc
     3961 ctgcaggagc aactgcaaaa gcagaatgat tatctgtcca ttcttcatca ggtgacactt
     4021 gacctgctta atcgccgcga tctaacagac cttttgaaga cgattgtcaa tcattccgct
     4081 ttgttgttgg atgcgcctct cagtgaattg atgctaattg aaggcgacaa actggtggtg
     4141 aaggcggtga cggataacca actggatctc ctggaggaaa gggttggtcg cgatcaggca
     4201 tctctttcat ggaaagcatt tgatacgctt caacccgtcc tgcttgaaga ttattcgacc
     4261 tggtcagggc acagacctaa atatgatgat tttcaattac acgccgtggc tgattttcct
     4321 gttgtggcag gagatcattg ttttggtgtg cttgcccttg gcaggaataa acccgattat
     4381 ccctttaccc ctgaacagac ccagacaggt attttgtttg cccgcttggt tgcactcgtg
     4441 ctcgacaatg tgaatctcta tgactcagcc atgcaggaga tcatggaacg cgaacgtgta
     4501 caggcatcct tgcagcgttc gaaccagcag caacaggtca tcaactcgct cttgaaagtg
     4561 agcctcgaaa atcgctcaat ggatgaagtc ctcggctcca ttttggatga gatcctttca
     4621 attgactggc tgaccatttc tccgaaaggc ggcgttttcc tgttcaataa gcagaccaac
     4681 ttgttggaac ttcgaaccca ccgcaatctt tcgccgcatt tacagtcatt gtgtgccggt
     4741 gtggctttgg ggcattgcct gtgcggacgc gccgcgcaaa cccgccagat ccagttcgca
     4801 gattgccttg atgaacgcca cgagactcgg tatgaaggca ttgaggaaca tggtcattac
     4861 aatattccga ttgttgatgg tgaagatatc ctgggtgtga tcgttttgta tttgccgcat
     4921 ggatatcaaa aatcggaaaa cgaccttgtc ttcctgaatt cagtggcaga tgctgtgaag
     4981 ggtatcatgc agcgcaagca ggcggagacc ctgcttctgg aaagtgagat ccgtttccgt
     5041 caaattgttg aaaatgccag cgatatcatt tatcgtgccg atatcaacgg caatttcaca
     5101 tatgccaatc catccgcatt gaagatgatg ggctttatga gcgaaaaaga tgtgatcgga
     5161 aaaaattatc ttgatctgac cacatctgag tatcgtcaaa aattgaagcg cgtgtacgat
     5221 catcaataca tcagcaagac catgagcacg tatcacgaat tccctgccgt ggctgctgat
     5281 ggaggtattg tttgggttgg gcaaaacgtt caactcatca tggatggcga taagatcatc
     5341 gggtttcagg ctgtggcgcg taacattacc cagcttaaac aggcacagga agccctggcc
     5401 ttatctcgcg atcaagccct ggaggccagc cgtttcaaga gtcagcttgt ctcaagggtc
     5461 agccatgaat tgcgcactcc attgggcggc attcttggct acgctgagtt acttcaagtg
     5521 gaagcctttg gcggacttaa tgaaaaacaa caagccgcgc tcaaaaatat cgttgagagc
     5581 acaaactttc ttaccaccac ggtcaatgac ttgctcgatc aggcccagat cgaatccaaa
     5641 tcgcttagct tgcacaatgc ttatttcagc ccggcgacat tgctctcaaa gatctctgcg
     5701 cccatgagtg tgttggctga gaaaaaggga ttaaaattcc gcactgaaat ttcaccatcc
     5761 ctgccgctgg aactgtatgg tgatgaaaaa cgcctgcagc aggtcatcat caatcttgct
     5821 ggcaatgcga tcaagtttac gagcgctggt gaagtctaca ttaatgtctc atctcccatc
     5881 cccgcttatt gggcgatcga agtgcgtgat acaggagtag gcatcccgga aaacgaacag
     5941 gcaaatatct ttgagccttt ccgccaggtc agtaattcca tcacacgcga gaaccgtggt
     6001 tcaggtctgg ggcttgccat cgtaaggcaa ttggtggaat tgatgggcgg ccaaatatcc
     6061 ctgcaaagtg aatcaggcaa aggtagtatc tttactgtta ccctgccgct tattaatgct
     6121 cctggagaat agtgatgcca attcaaccct atgcgatcgt tgtggaagat gacccaaagc
     6181 tcggagccat cttccaaacc accctgcaac atgcgggatt tgaaaccgcc ctcgatcaca
     6241 atggcgacaa atatcaagcg tttctaaata tcgccgaacc tgctcttgtg atcctcgatc
     6301 ttcatctccc ctttgcattt ggtggcgata ttctggaggt gattcgtgcc cgctacccca
     6361 atacggtcgt tgcaatcgtc accgcagatc tttataaagc caaggctttc aacggcaaag
     6421 ctgaccatat tctcattaaa ccggtcagtg ttgcgagcct tttgaaactc gccgaatcag
     6481 tgaaaggatg aactagtctt atgcccaaac cccttgcgct cgtaattgaa gatgacccgc
     6541 aactcaacca gattttcacg ctaaccctgt tcgaatcctt tgaagttgaa actatttcag
     6601 atggttccca agccctggat cggctgactg aaattattcc caatgttgtt gtccttgata
     6661 ttaacctgcc cggagtgccc ggtgaccaga ttcttagaat gatccgctcg aattcgcgtt
     6721 tgcattcggt gaaggtcata ttggcgactg ccagcacgca ccaagccaga gaccttcaag
     6781 acatggccga tatcgtgctt ttgaaaccga tcagccccat gcagctgcgg gagcttgccg
     6841 aacggcttag tcgtatgggg tagggtttcg attggcgaaa ttggaaaagg ctgggaattt
     6901 cagtcaaagt aaatattcag gtctgaacaa tcaaagcggt ttccacaatt ggggcaactg
     6961 accttgcaat tcttttcata catttctgtc ccacagcgat cgcagatcca tacccgttgt
     7021 tcgttccatg taatctcatg aaaaagggat gggttgaagt ttcggatacc atccttgccc
     7081 agaatgacga cctgattcat gaccctttca gcatctttcc tgaattgttc caccccaata
     7141 cccctgcaaa tatccggcca atctttcagc catttcaaac tccgtccata caccttgact
     7201 gcgccctcgt aatttccgcg ggtaatgtgc aagtatgcca ccgcaacttg aaggatgccg
     7261 cgatacaact cgcggaccgg gcctgtttct tcgcgccatg catcttcaag cgcttcgtgg
     7321 gcttcgaagt actttctctg gttgaacaac cgtaacccct caatggcttg cggatggagc
     7381 ggggaggaac aggaattttt cataaaacag ttctgtagta tggtttgggc cgtgtctttc
     7441 aaatggtaga attaaaccac aatgcgaaag caacttgaag acagacgctg gggaattgcc
     7501 ctggcatttt ttgcgctgat ttccctggtt ttgctggcgg gcgcattgca aaatgcaaac
     7561 tttctgcccg cccagccgtt gggtggctct gaaagcgacc aaatcaccat tgacctttct
     7621 gtgctgaaag acgcgttggt taattcgggg gaaattcctc tttggaaaca atttgtattt
     7681 ttcggggcca ttttcctttt tctttcactg ttggcgctgt ttctctcccc tgagctgcgc
     7741 aaaaaactgt tcttaatgct gctgcgcctg ctggcctcag tgattatggt gtattacctg
     7801 ttgaaggtca agccggatat tttttcaggg ctattgcagt ttggtcaact ggggcaatca
     7861 gacggacaga ttcaggatgt gcccgcacct gtttttcaac ctccacagat atcatcagcc
     7921 acaacctttt tgttgaccct ggcttttgtg gttctggtcg catttctgtt ctggctgatc
     7981 aatcgctggt ggacaaggca aaaggaattg atcgcaaagc gaagaccact tgccgaaatt
     8041 gcagcgattg cacgcgcatc gctgcatgat cttgcctctg cttcaaattc gaatgatgcc
     8101 attattcaat gttatgtccg catgagcaat gtggtggcga tgaaacgggg actgcagcgt
     8161 cagtatgcca tgacccctgc cgaatttgcg gcgtatcttg aaggggcagg cattcctccc
     8221 gagccgatct cccgtttaac ggggctgttc gaggcggttc gttatggcgg gcatatttca
     8281 ggtccacggg agatcgatga agcctccgac tgtctgaaat ccatccttcg atattgtggc
     8341 gaggccgtat cgtgaagcgc cgctatttat tgcttgccct gttgatggtt ggagcggtgg
     8401 tggtctcgta cttgttgaag gaagtgatct atcaaactgt ttttgtgcca ttggcgtact
     8461 tgtggtggtt gtttggtttg tattatcgtg ccgtgccgca gctgatcctg tggattttgc
     8521 ttgtggtcat ggtttttatt tcctctctga aactggttcc cattatcaat tttttccctg
     8581 cggtagaaaa aaagatcaag aatgaaacag tgggcccggt ggagcagctt tccatgatgc
     8641 tcaataagcc gtcccgtggc gtttattaca agtggatgat cgccaaccgt ttaggcaggg
     8701 tggcgcgtga aatgctcgac caacgcgaag gacgtacgtt caaaggatat tcgcgtttgc
     8761 gcggcaggga ttggactccg cctgccgatg tagatgaata cctcgaaacc gggttgaacg
     8821 gctcttttgc cgattaccca accccgcact ggtggcggga ggcagaacca accccgctcg
     8881 accttgaccc gcaaaaagca attgactatc ttgagaacga gatggagatg aatcgtgacc
     8941 gaaatcgtaa aagcatctga actgagtaaa caggttatct ctgaagtgga gcgtgccatc
     9001 gtaggcaagc gcagtttgct tgaaatgatc atggcggctg ttctggcggg cgggcatgtt
     9061 ttattggaag attaccccgg ccttggcaaa accctgatcg cacgcagctt tgcggcggca
     9121 ctcgggttgg atttcaagcg gattcaattt acgcccgatc ttttgcccgg cgatattacc
     9181 ggtggctata ttttcaatcg gacaaagaac aagttcgagt taaggcaggg acccgttttt
     9241 accaacatcc tgcttgcaga tgagatcaat cgcgcttcgc ccaagaccca atctgctttg
     9301 ttggaagcca tgcaggaagg tcaagtgact ctggaaggcg agagtatgaa actgcccgat
     9361 ccgttcattg tgctggcgac acaaaacccg atcgaatacg agggcacttt cccgctgccc
     9421 gaagcgcaac tggatcgttt catgttgaag ttgaccgtgg ggtaccccga ccttgaagaa
     9481 gagcgggaga tcctcaaacg ccgccgcgaa cgcaagcagg atgaacttgc gctcaaacaa
     9541 gtcatcaagc cgaaacagtt gctcgaaatg cgtgccgcgc ttgaatcggt gcatgtggat
     9601 tctgatcttg aggggtacat ctctgcgctg gtgcatgcca cgcgcaccga tcgccgtgtg
     9661 tctgttggag ccagtccgcg cggatcgctg gcgtttctaa aaatggcgcg tgccagtgcc
     9721 gtactggctg ggcgggattt cattttgccc gatgatatta aacgctttgc cgtgcccgtg
     9781 ttgagtcatc gccttttgct gcagcccgaa tattggatgt cacagcaggt taacggcgat
     9841 gtcattcgtg atgtattgag ccacactccc gttcccgtca taaaataact tgtatcgcta
     9901 cgtcctcctt tctgcgttgg tgtatggatt gctgcttgca gggttggcca ccctgcaggg
     9961 cacttttgtt gcgcttgcga ttcctttcat gctgttcctc gtttatggtt ttttgaatgc
    10021 gcccgaagag ttgaatattc agattcaacg cacgctttcc accgaccgtg cttcagccga
    10081 tgcggatgtg gtcgtgactt tgaaagtcac caacaacggc gcagcaattg aggaactttt
    10141 actggatgac aggatttctc caaacctgac cttgcgaaac ggatcgcccc gacacttgct
    10201 gaggctcgcg aaggacagca cctgcaccct gacatattct gtcgcaggga ctcgcggcat
    10261 gtaccacttt gaccagattc atctcacggt tactgaaccg cttggattga ttcgccgtga
    10321 gcagaccgtc catgctgaaa aacggttgac catcttcccc ttggtgacaa agtttaaatc
    10381 ggtcgccatt caccctctgc ggactcgggt ttatgctggg accattcccg ggcgcattgg
    10441 aggcccaggg acagagttct ttggtgtgcg tgaatatcaa atgggcgatt ctccccgcac
    10501 gatcaattgg cgcgccagtg ccagacaccc cggtaatttg ttttcaaacg aatatcaaca
    10561 ggaacgtgtg gcggatgtcg gtgtggtgtt ggatgcccgt gccagtgcca acatctttca
    10621 tgtggatcaa tcgttgttcg agtattcggt ggctgccgct gcaggtcttg cggatgcgtt
    10681 cttggcgcag ggcaaccgtg tcggcttgtt ggtttatggc tcgtatcttg gttggaccat
    10741 gcccgggtat gggaaaattc agcgtgaacg catcatgcgt gccttggcag gggcagaggt
    10801 gggtgtgagc tccgtctttt cgggcttgca gcatttatcg ccgcacatgt tcccgcctga
    10861 atcgcagatc gtgctggtta gtccgctggt aaatgatgat cttgaggttt tgattcaact
    10921 gcgtggacgc ggctatcaag tgatggtcat cagcccagac cctgcgcgct ttgaaagccg
    10981 ccttttggcc gaccacccca aagtggattt ggcggaacgc attgttcgca tggagcgcag
    11041 cctgttgctg cgacgattgg aacgggcggg gattcaagtc atcgggtggg atgtgtcgat
    11101 tccgctggat caggccatcg gtccattttt gacacggccc catcggtccg ttttcaggag
    11161 gtcatcgtga cagaatttgt acttgtggcc agcatcgtct taagtgcggg gtcgctggct
    11221 tgggtgtttt ttgaagcagg cttgagcgat tttgggcgct gggttttgat ctttggtgtg
    11281 gcatggttgg tggcacagcg ggttggttgg aagtggattt cctcagtggg gctggtgttg
    11341 gcggtccttc ttgcagcgac tggcctgtgg tttgggctta ctccaggctg gatgtttgca
    11401 ggggcgattt tcgccctcgc cgcatgggac ttgaccgatt tccgcgaccg cctgcgtttt
    11461 gtcgctgtcg atgacaatgc gcgcggcatg gaacggcgtc atctggcgcg catcagttgg
    11521 ctggtgttgg tggggatggc acttgcctcg atcacgatgg ttctgcaagt caaattcacc
    11581 attgaatggg gtgcgctgct ggtggtggtg atcttgatcg ggctggggca gttggtcaat
    11641 tggttgagga aataattttg ctttgaacga tactgcttcg cttatgctga aatggccgcc
    11701 agccattttt cggcttcctg cggatgttcg aattggatga tcgtcaggtg ggcattttcg
    11761 ggttgtgaga acaggtttgg gtattcgcgt tttctgcgcc agtaggtttt gaacagccag
    11821 ttgaaaagcg aatcttgcga ccagaatttt atttgtaccc agaaattttc aacgttatct
    11881 gaaaagagtt tttcttttgt tgccacgcgc cgaaatgtgc gggtgaacaa ccgccagaaa
    11941 acaatcggga aggaataatt gagccagatg atcgcctcgg cttgcggcca gatgagatcg
    12001 cgggttgcgc tgtaatttcc cgccaccacc cagctttcgg cgctgatggc tttttctacc
    12061 cgttcacgga atacgtcgtt gggggcttcc acccaattgg gctcccagtg caggtaatca
    12121 aggtcaatgc aaatggcgcc gattttctcc gccagagcct tcgccagcgt ggattttccc
    12181 gatgaggtgg tgccgacaat aacaacttta cggtagggaa atggtttcat aatgggcaga
    12241 gtataatttg gctgtcataa atcgtaaact tgtgctgaac aagtcgaaat atttcaaatc
    12301 gaaaatcaaa aatccaaatg aggctccatg tttgaacttc ctgatgatga aattctgaat
    12361 ctctcaaaag aacacgtctt ctggacgtgg tcggcgcaag ccaaagtgaa tccaattgcc
    12421 gtgaagaaag ccaaaggcgt ttacttctgg gatgtggatg ataagaagta cctcgatttc
    12481 aactcgatga ccatgtgtgt gaatattggt catggcaacg aacgtgtcat caaagccatg
    12541 caggagcagg ccgcggaatt gccctatgcc gcgccaggca tggctacaaa gatccgcgca
    12601 ttggcaagca aggcggtggc agagatcacc cccaaaggcg cgttgagcaa agtccttttc
    12661 acgcttggcg gcgcagatgc aaatgaaaac gccatcaagc tggcgcgcgg ttatacaaaa
    12721 cggcacaaga tcctcacgcg ctatcgttcg tatcacgggg cctcagcagg agcgatggcc
    12781 gcgacgggcg atccgcgccg tgtggcttgg gagccgaacc tgatgacggg ggtggtgcat
    12841 ttcctcgacc catatcgtta tcgctccacg ttccatcgca cgaaccccaa catttccgaa
    12901 gaggaattca cccgcgatta cctcaaccac cttgaagaga tcattctcta cgaaggccca
    12961 gaatccattg cggctatttt gatggaatcg gtgacaggca cgaatggaat catcattccg
    13021 ccgaatgggt acatgcaggg tgtccgtgcg ttgtgtgata aatatggcat tgtgatgatc
    13081 tgtgacgaag tgatgagcgg gtttgggcgc actggcaaaa tgttcgcgat tgaacactgg
    13141 gatgttgtgc ctgatatcat gaccatggca aaagggctta cttctgcgta tgcgccgctt
    13201 ggtgcagtgg cgatgaagcc agaaattgcc gcggctttta atgagcacgc ctttgaaagc
    13261 ggcctgactt atacatcgca tcccatttca ttggcggcgg cagttgccaa cattgaagtg
    13321 atgaaagaag aaaggatcgt tgaaaatgca gctgcgatgg ggggcacact tcgccgaatg
    13381 cttaccgacc ttggcgaagc gcatccgtca gtgggcgagg ttcgcaacat cggcctgttc
    13441 ggcattctcg agttggtgaa agaccgcagc accaaggagc ccatggcccc gtggaacggg
    13501 tcatcgcctg agatgaacgc gcttaaaaaa tattgcaccg atcatggcct gtttttgtac
    13561 acccactggc ataccgtcct catcattccg cctttgatca ttaccgagga agaactaaaa
    13621 gaaggaatgg atgttctgga caaggctttg gaaatcaccg acaaagttgt gaaataaaaa
    13681 aaacaggtca cgtttcaaac gtgacctgtt ggtatttcga tcgtaaatgt cgtccccctg
    13741 ccgatctcgc tttcgacttc gatcgtcccg ttgtgcgcgt ggacgatctg ttttgcaatg
    13801 gccagcccca gcccgctgtg aacccgccct gtgcgtgatt tatctccgcg ccagaatcgg
    13861 tcaaagatga aaggcagatc ttcggatgca attccctcgc ctgaatcttt gaccttgatc
    13921 cgcacgcttc ccgcttcact gaggcctgat tctgtttcaa gagaaatagt cccacctttg
    13981 ggcgtgtggc ggagggcatt ggaaatgaga ttcgacagca cctgattcag cctgtcgtaa
    14041 tcagcggtga tttcctgggt tgggttggcg agttcggtgg tcaggtcaat gccaagggtg
    14101 gcggcttgtg cggaaaaact tgcggtgaga tcatgcgtca ggtcagcaag caggaaacgg
    14161 gtggggtgaa gcggaagttg tcctgattct gccagtgaga gggtttgcag gtcgttgacc
    14221 aggcgggcga gaagtttggt ctcatccaga gtgttgttga tgtgctcggt cgtcggctcg
    14281 tacacgccat cgatgatgcc ttcgaggttg ccttgaataa tgtgcagagg tgtgcggagt
    14341 tcgtgggcaa tgtcagatgt aaggttgcgg cgctgctggt cggcgcgttc gagttcggcg
    14401 atcatcttgt tgaagcgggt gatgagttcg ctgaacatgt ctgatctgtt ttctggcaca
    14461 cgcactgaaa gatcgccttc ggcaacagag tcgatcgcgt tgaatatctg gctcaatggt
    14521 ttgccgaaac gcatgtacat ggagaagacc gcaaaaacac cgagcatgat cactgcaaca
    14581 ggcgtgcagc ataaaagaag ccagatgttt ctaacccctg catattccga gaaggcccag
    14641 tacaggatgg cggctgtgcc tgcaatgaaa agcatgacag tgccaagaaa gaagaacgcg
    14701 aacggaaaaa aacgacgccc tccgcgccag cggcgttgga aatgaggcgg ttgattttgg
    14761 ggagggcttt gatgagacat gcgaggtgtg tgggttaccg ttcgataaac cgatagccaa
    14821 tgccataaat ggtttcaatg gatgggctgt ctgattcggc ttccagtttc tttctcaggt
    14881 ttttgatgtg cgagtcaacg ctgcggtcaa tgctggatgc ggcgccgtgc aggtcttcga
    14941 gcaattgctc gcgggtgagc gtttggcccg ggcggctggc aaggatggag agcagcttga
    15001 attcgttggg cgtaagtttg atgggtgttc cgttgagtgt gacggaatac ttctctgaat
    15061 cgatctccag cccgccgatt cgaattgtgg tcggggtttt gatgccgcct ttgctgcggc
    15121 ggagcaaggc acgcacacgg gcaacgagcg cacgcggcga aaatggcttg gtgatgtaat
    15181 catcagcgcc gatttcaagg ccagtgacct ggtcaatttc ttcggcgagg gcggtcagca
    15241 tgatgatggg aacgtcgctt tcgcggcgca agatcttgca gacctcgcgc ccgtccatgt
    15301 tgggaagcat gaggtcaagg acgataaggt cgggtttctc acggcgggcg gtggccaacg
    15361 ccgattgacc gtcactcgcc gtgaggacat gatatccgtt cttttcaaga taatcccgtg
    15421 caaggcgggt gatcttgggt tcgtcatcta caacaaggat gatttcagac atgggggcag
    15481 tataagggat gaagggtgaa agatgaagga tgaaaattgg gagggcgatt actttttcca
    15541 gatctcccat tttccatcgc caactgtcgg aaggtcttct gccagccatt cgaacatgga
    15601 gccgtgatac acagtgacgt cttcgtaccc agtcatcagg tgggccactg cattgactgt
    15661 tgcagcgatt cctcctccgc agtaggtaat gacacgctta ttgtgtgtca cttcttgtaa
    15721 gtgggcggca agttggtcgt cgggcaggaa ggcatccagt tgctgcatca gattcatgga
    15781 ggaaaggcag atcgagcctt tgatgtgcct ggcgtcgtag ctttcgcgag gcattacgtt
    15841 caccacggcg atgtcggttt ctttcagaga cttcaatact tcttccttgt ctgcgtacat
    15901 ccactttctc aggctgggct ggaaattggc aggctcataa ctgtggattt cctgttcggt
    15961 ttgcccgcct gcctgcttcc atgcagagag tccgccgttc aggatgcgga ccttgtcatg
    16021 ccctgcgtag cgaagcaccc accaggctct cacggcatat gggagcattc cgcaggcata
    16081 gaccaccacc tctgtttcat tggaaatgcc aagagcaccg atctgttcgg cgagtttgtc
    16141 ctctggcagc attgtggtgt aacggttggc gctttcaggc gcagagaaat tttcatgctc
    16201 aaggaaagcg gcgccaggga tgtgctcctg caggtaggca tcgtctgtaa tggtggcatc
    16261 aaagatgcgg atatttttat cgcccagttt cttcaacagt tcatcggctt cgatcagaat
    16321 gtcatttcgg ttcatggcag tgcctccatt ggatttagtg ttatcgaaac tatatcaacg
    16381 ctttgtggag gaaatatgga cgtctccgcc agaaaatgtg gaagtttagg cggggaaaat
    16441 aaaaagagga aagaagatgg gcttacattc ttctttcctc tttctcttgc cgatcacttt
    16501 ttacagatcg tcggtcttac tcgctcttct tttcttcagc gggtttttcg ccgtcagcgg
    16561 caggagtttc gccgtgagca cgtttgtgcc actcgttgaa catgggcggg acgccgcctt
    16621 cccagttctt gccccacggg ccgtggccgc catgatggcc ccagccgtgg cgcattctgc
    16681 ggaagaacat catcttcatg aagccgaaga agaagaacag caggaaaatg ccgaagcaga
    16741 tcccgccgag cgggaaaaag ccgaagtgtc catggccatg cattccatag ccgtagccat
    16801 aaggcgcaaa gccgtgacca tacaccatgg gcggtacggc ctgcccatct tcagcggcct
    16861 gtgcgatggc ggttgccaca gctggagcct gtgaaattcc ctgtgccatg cctgctttat
    16921 aggcaaaggc acctgcgcca gcgatcagcc cgagaacgag cagaaagcca atgacgcgaa
    16981 acaaaataaa cggtcctttt ttcattttta cctctcagag ttgaattgat ttggtttgtt
    17041 cttacgcccc taatttatca atccattgtg gaggaattat ggatgggtgg gcgagagatt
    17101 gtggaggctg tgtaggaaaa gtgttaactg gaacgcctcc agcctgagac tttgcaactt
    17161 ttcctgcggg cacgcatctg ataaaatgca ggtcggtaat agcaggcctg aatgggtcgt
    17221 cttgtatacg gttgataaca aatttcaggt gaaaatcacc ttaggatagg aaaacgaaaa
    17281 atgacagaga agcatgtaaa ggtattggta ttgggttctg gccctgctgg gctttcggcg
    17341 gcgctgtacg cagcgcgggc agaattggag ccgatcgttc tgacaggaat gcagttgggc
    17401 ggccaggccg ctctgacgca cctcattgaa aactaccctg gttttcctga aggtgtgggc
    17461 ggtgcgcagt tgggcgagtt gttccaaaag caggccgaac atttcggcgc gaaggttgag
    17521 ttcaatatgg cgcacgaagt ggatctttca cagcgcccat ttacggtcaa gaccgacgat
    17581 ggtgattaca aggccgaaac cctcattgtg accaccggag cgaacccaaa ccatttggag
    17641 atcccaggtg aggtggaact gactggtcgc ggcgtttcct attgcgccac ctgtgacggc
    17701 tggttcttca aagataaaaa ggtcgtcatt gtgggcggcg gcgatagcgc cttcgaagaa
    17761 gcgctcttca tcacccgcta cgcttcctct gtcacgctca ttcatcgccg cgaagatttc
    17821 cgcgcgggcg ccattttgca gaaacgcgcc aaagaacacc ccaagatgaa ttttattttg
    17881 aacacggtcg tcactgaagt gctgggcagt gacaagcttg aagccttgaa gctcaagaac
    17941 gtgcagacgg gcgaggagtc gatttttgaa actgacggcc tgttcatttt catcggtcac
    18001 acccccaatt cgcaaatgtt caaaggtcag cttgaaatga gcgacctcgg ctatgttctg
    18061 gtaaatgaca agatggaagc cagtgtcgaa ggtgtgtatg cggctggcga aattgccgac
    18121 ccgcacttca ggcaggtcat cacttcggcg ggcatgggtg ccgctgccgc cattcaggcg
    18181 acgaaattcc tcgaggaaaa agaagcagtt cacagttaag ggcgaagcga tgaaaatcgc
    18241 gcttccactt tgacgggtgg cctgattctg accgaagggc agtctctaac gggtggaagt
    18301 tcctcaataa agactcgtga aagcgggtct tttttgtgcg taaaaaaagt gacattttgt
    18361 acttttgacc catgccactt tcggatagaa ttagtgaaaa atcacggcgt ttttcgccga
    18421 ccaaacccca ttggaggaac acatgaagaa aatctcaatt atcggcgctg gcaatacggg
    18481 cgcaaccgcc gcgcattggc tcgccgagcg cgaacttgct gatgttgtct tgctggacgt
    18541 ggtggaggga atgccgcagg gcaaaagcct cgacatgttg gaggccatgc cgatcatcgg
    18601 aaaagatacg catgtggtcg gcacgaacga ttatgccgat acaaagaatt cagatatcat
    18661 catcattacg gctggcattg cccgcaagcc tggcatgagc cgtgatgacc ttctgaagac
    18721 caatgccgac attgttggca aggccgccat ggagaccctc aagcactctc cgaacgccat
    18781 ttttattgtt ttgaccaatc cgctcgacac catggcttat ctcaccatga agatgaccaa
    18841 gctgccgcgt gaacgtgtca tcgggcaggc gggcattttg gattcagccc gcatgcgtgc
    18901 ctttgttgcc atggaaactg gggtgagcgt ggagaatatc aactgctatg tgttgggcgg
    18961 tcacggtgat gaaatggttc ccctgacccg tcattccaac attgcaggca ttcctttgaa
    19021 ggaatatctg cccgctgaca agcttgaggc cattgtcaac cgcacccgca agggcggcgg
    19081 cgagatcgtg aacctgctca agacgggttc cgcttactat gcgccttcca tggcttgtgt
    19141 gcaaatggct gaagccattt tgaaagacaa gaagttgatc gtgccttgcg ccaccatgat
    19201 gcagggtgaa tacggtttga acgatatgta ctttggcgtg cctgtggtgc tgggcgcagg
    19261 cggcatggaa agagtcgtcg aatacaagtt cgatgccgaa gaaaaggcca tgttcgagaa
    19321 atctgcggct tctgtgaagg aaacgcacga agcattgaag ggtttggtta cattctaatt
    19381 tgatgggtca cggcttttgc cgtgacccta cctttggagg aacaaatgat tttgcatgaa
    19441 aaactctctg ctcaactgcc cgcatggcgc gaacgcatca agtctcttgc caaggaacat
    19501 gccgaagtgg ttgtggacca tgtcaacatc ggccagatcg tgggcggcat gcgcgatatc
    19561 aagtccctgc tttctgatgt ctcttttgtt gaccccgcac acgggatcat cttccgcggc
    19621 ttatccatcc ctgagttgtt gaaggcgctg cccaaggcgc gcgccagcaa aatgcctttg
    19681 gtgggcggtc tgtattattt gttgatgatc ggcgaagtgc cgaccaagga acaggcgctc
    19741 gaagtggaag ccgaatgggc caagcgcgcc accgttcctg actatgtgta taagatgctc
    19801 aagaccatgc cgaaagagac tcacccgatg actctcttct cgcaggctgt ccttgcgctt
    19861 cagaacggct cgaactttgc ggccaagtat cacagcatga agaaggatgt ttattgggaa
    19921 gccgcccttg aagacagcct cgacctgacc gcgaagctcc ccgtcatcgc cgcgtatatt
    19981 taccgcatga aatattatgg cgagacctcc aagccgaaat acaaccccaa gctcgattac
    20041 ggtgcgaatt tctccaagat gatgaaggtt tcagacaaga agggttattc cgaacttgcg
    20101 cgcctgtatt tcatcttgca cagtgaccat gaggctggga atgtctccgc ccataccatg
    20161 cacctcgttg ggtctgctct ctccgacccg tacctttcct tctccgcctc gctcaacggt
    20221 cttgctggac ctttgcatgg tctggccaat caagagtgcc tcggctggct gctggaagta
    20281 cacaagaaat tcggcggtgt gccttcccgc gaggacctgt acaaattcgc ctgggatacc
    20341 ctcaacggcg gtcatgtcat ccctgggtat ggtcatgcgg tcttgcgcgt tcccgatccg
    20401 cgttttatgg cgcagatgga attcgccaag aagcgcttcc ctgatgacga attagtacgc
    20461 cttgccgacc tcgtcttcga tgtggtgcct gccgtcctca aggaacaggg caaggccaag
    20521 aaccccgccc cgaatgtgga tgcgatcagc ggtaccttgc agtattacta cggtgtgcgt
    20581 gaatttgatt tctacaccgt gttgttcggg gttggccgtg cgctcggcgt tactgccaac
    20641 tacgtttggg ctcgtgcgct cggcatgccg atcgaacgtc ccaagtcgtt gaccaccaag
    20701 atgctggaag atgtggtcgc aaaagccgcc caacagcctg catagtcaat agaatcaatc
    20761 tcccgaagtg aaaacttcgg gagattttta tttaaatcag gggtgttgtg atggtttatg
    20821 gttttgcgcc caacctgtcg atcaggaacg caaaatcaag tacattttct ttcagatagt
    20881 cataccttcc actggcgcct gcgtggcctg cgctgaaatt tgtttgaagc agggcgaggt
    20941 tgtcgtcgtt ctttttatcc cgcaggcgtg caaggaattt ggctggttcc caataggcca
    21001 cccgtggatc gttgaaccct gtggagagca gcaggtgcgg atattccact tcccgtagat
    21061 tgtcgtatgg agaataggac agcatgtagt cgtattcatc cctgttcgcg gggttgcccc
    21121 attgatcgta ttcgagcgtg gtcagcggga tgcttgggtc actcatgctg gtgatcacat
    21181 ctacaaatgg gactttgcag atcactgcct cgaaaagctc tggccgcatc accatgcttg
    21241 cgccgaccag gagtccacct gcggagccgc ctgtgctggc gagcttttcg ggagaggtga
    21301 atccttctgc gatcaagtgc tcggcgcagg cgatgaaatc ggtgaatgag ttctttttgt
    21361 tgaacatcct gccttcgtcg taccagaaac gtcccatttc ggagccgccg cgcacgtgag
    21421 caatggcaaa gacaaatcca cggtcgaaca ggctcaaaag gctgaggttg aatgccgcgt
    21481 cgctggaggc gccataggct ccatacccat ataacaaggt tgggttttga ccgtcttttt
    21541 tcaatccctt tttgtaggca agggaaattg gaatcttgcg cccatccgct gcggtggcat
    21601 ggatatattc cgtgacgtat tgtgatttgt catagccgcc tgggatttga tattcctttt
    21661 tcacctccca ctcgccgtta tccatgtgaa agtcaaccgt agagaagggg gtggtcaacg
    21721 aggagtagcg caggcgtaac aggttcgtgt ggaattcagg gttgacatca acaatgacct
    21781 cgtaagtggg gtcggggaag ttgacataac ggacatcgct gtgaccatct ggcctgctga
    21841 ctctgagttg tttgagtccg ccttttcttt cgtgaaggac gatgtgctcc gcgaagacat
    21901 ccacgtgttc gagcagggta tcttcacggt gaccgatgac ctcctgccag ttttccctgc
    21961 ttggggcggc agtgggtgtt ttcatcagct tgaaattatg cgcgtcttca ttggtgacga
    22021 tatagaaatg gtcatcaagg tgggcagcgt aatattccag ccctgctttg cgtggttgaa
    22081 ggacattcag cgccccgaca gggtggtcgg ctggcaggaa gcgcatctct tgtgaaatcg
    22141 tattgtaatg ataggtgaag atgtattttt cactacgaga tttgaagagt gaaagagaga
    22201 aggtgtcgtc ttcttcgaag tacaccagca cgtcctgtga tggcgccgta cccactttgt
    22261 gacggtagag tttacaggca cgatgcgatt catccaggct gagatagaac actgtttcgt
    22321 tgtcgttggc ccattccacg cctgcctgaa aataaacact gccatggata ttggcaatgg
    22381 cttcggggta cattgtgccg ctgatgaggt ctttgacatg cagggtgtag acctcagagc
    22441 cttcaaggtc aacggaataa gccagcttgt tttggtctgg gctgacggaa aaggcgctga
    22501 cactgcaaaa ttcatgtccc tcggccagtg cgttttgatc caacaggatt tcctcggctg
    22561 cattggggat atttttttta cgacaaaaga tggggtattg tttctctgct tctgtgcggg
    22621 tgtagtacag gaagtcacca tgtttttcgg ggacactgtg gtccacctcg cggatgcgcg
    22681 ctttcatttc gtgaaagagc ttttcacgca gcggattcag gtgttgaaga gtttcttcga
    22741 ggtagtcatt ttcggcttgc agataggctt tgacctctgg gtcttcacgg ttgcgcatcc
    22801 agtagtattc gtcgttgcgg gtctgtccat gctgggtaag ggcatggtct cgtttgggtg
    22861 caattggcgg catgatgctc ctgtttgggt taacggtaat cttgtgagat tttttctaaa
    22921 ttgatcaggt tgctgtcgat tcggatgtag attccgtttt cgagtttttg gtattcttct
    22981 tttccgcgca taaaatcggc gaggggggcg aagttatctg cgatcaggtc gggacgaagg
    23041 tggatgatgc tgtagtcata gtcccatttg ttgtgacctg gatagaactc gccgtggccc
    23101 tggcctttgg ccacaatggg gtcgttgagt ccgagcaggt cgatggtggt gcgttgcgag
    23161 tagtatggaa tctgcccagc ggcgtggacg gcgatcacgg cttcgggtga ggtggattca
    23221 gcgaggaaga gtcccagccg tacgcggcgg atatccgctt tgaggagggg ggcgttgtca
    23281 acggtgtagg tgaaccatgg ctcgccgctg acgatgagca gggtgatgac cgcaagtgga
    23341 atggcaggat tcactttcgg ggcggtgaat gctgattggg gctgggccgc ttgcaggtcg
    23401 gtgaggatgc ggttcgtcat ccaactgaaa aggatgatga gggcgggcat tccctgggtg
    23461 atgaagcggt tggcggcgtt cgtttcaggc tctgcataat caccgccaac atagatggag
    23521 taggcagatt gagcgaggaa gatgccgagc agtaaaagtg tttcgcggct gcgttgcagt
    23581 ttatagaaga agatgcctgc cagtgaaaac aaaaagagcg gaagtgtatc gcgtgcggcg
    23641 aattcgacga agaccagcag cccgtggcgg atgcgttcga gggcggtgaa ccctgtcatt
    23701 ttttggtaat aggtggcggg aagaaaatcg ccgaagtaat tgtgttgaaa atagaaaacg
    23761 gccagcaatg tgatggcggc tgatgcgatg gggaggatgg cattgcgttt gtttttgagc
    23821 agcagataaa gcgcgatggg tgcaatggaa atgatcgcat ccacgcggac gagaatcgcc
    23881 gctgcaaata tgaggccgat aaaaacgggg tgtttctcgg tgatcgagaa aagaactgca
    23941 agactgacga gcaaggcaag caagcccact tccatgccac gcagtgacca gaacaccagc
    24001 ggaaaataaa atgccgtgat gagggctgcc agtgtgggcg cgtattttga atcgggttgg
    24061 agcgcttctg caattttgga aactgtgtag ctgtttgcga gcaaaatcgc cagcgaggtg
    24121 agcatgacca caagggagat tttcgaggct ggaaacggga agagatgcag aaaagcgaga
    24181 tagagcgtcc agcccaggtt ggtgaagcct tccacaggcg cttcgccgat attccatacc
    24241 aacccgtgac cttgtgcgag attgcgggcg tagcgcatgg agatcatggc gtcatctacg
    24301 agggtgaaat agcgctcgcc tttgatatcg aaggaggtgc cgatgatgaa aatgagatag
    24361 aagacggcag aagtgataag aatgattttg agcggggaat gcttttccat gagttgtggg
    24421 attatatcgc acgggaggga aagcaaaagg ctgacgaaac atcgccagcc tttgaagatt
    24481 ttagtccgtg ctttggagtt ttttacctga cttgcggagc cagcgccgtc aattctgtgc
    24541 gcagcgagtt gatgatggcc acgttgccgc tttcatcgcc gtgcctggcg agttgttctt
    24601 ttttatgggc cagttctccg catgcttgat agaagcgtga ggtggctctg ccgctcaagc
    24661 ctgccatggt cagggtccac ggggagagcg ctctttggta atgcgctgct gaaattattc
    24721 cgttcgtcac ttcatcgtaa agctgccgct tgacgatatt gcgctcgttg ttgatcatcc
    24781 agatgataaa gccgagcatg aggccccagc cgatccaatc tacgaaggtg cctgcgaaaa
    24841 ggccttcgaa accgccgatc agcccgccga aggtgttgtg aaaggcatgg gtggtgactg
    24901 ccacaccgta gccgagaaag ggtgcgatga atttgatcaa cgggttgcgt tgggtgcgtg
    24961 aaaaggcaaa tccgatccct gtgaaagagg tgtagaacgg atgctgccat cccaccagaa
    25021 tgacgcggat gaagacgagc acgatcaggc cgccccagcc gttttcctga tatccgttgc
    25081 ggtaaatgta gagtgtgttt tctgtggcgg cgaacccgag cgccacaatg ccgccataaa
    25141 taatgccatc gaggatggtg tcaaattcct tgcggaacat aaaaaataca attgccactg
    25201 cgaggccctt gagaaattcc tcaatgatgg gggcaacgat ggatgtggtt ccaaagtccg
    25261 cggctccttc agagcccgtg aacatgaaaa tgccgatgcc aaaagccgtg ttgaggatgt
    25321 atgacccgcc agccgcgatc accacacccc aaaagaaagc cgcaccgagc aaaagtttcg
    25381 gctctttttc ataacggtca agccaataca caaaggccgc gaataaaaac atcggcacaa
    25441 aaccaaagaa tagtgaagcg agcagagcca tctgatttct ccttattctt gaaaaattaa
    25501 gcgggttctt tccacacgcg catattcagg ctcaatgcca agcgcggtca atacttcatc
    25561 ggggcggccc gtggcgcctt cacgtgcggc gagggtcatt tgcagggtgg aggattctat
    25621 atctgcggtc agggcttcga tgagcggacg caggtcgtag aattttccac gacgttcgcg
    25681 cggaatcgat tcggattcga gcaactctgc gaccctgcgc ttcagatcgg acccgtcaac
    25741 tggctctgtc aattggacat ggtattgcgc cgagaccacc tgtgtttgca gggcaggcgc
    25801 acgctcgtcc acgctttcga tgtttgtcac ctgaatatca tgtggaagat tatccttcaa
    25861 acgcaccctg atatcttcca gcggaatctc atcgttcagg cgtacatcca gcatttcaca
    25921 gcgtgaagag aagcccagcg gcagcgcggc ggcaaggctc atccgcggtt gggggtgaaa
    25981 gccttgtgag taggtgatgg gcaggtcggc gcggcgcatg gcgcgctccc agagacggtg
    26041 caggtcaagg tgacctgtgt aacgcagggc gccttgtttg gtaaatgtga tgcgaacacg
    26101 catgtttaat ttgattccgt tgcgatggat tttagccagg tctgaatttc gaggctggcg
    26161 cggcgataga ccttgtaggt gtgttccgca gtgcggtgga aaaggtcatg gtcgaggcat
    26221 tgattgtggg tcaggtgttc aaaggccgaa aggatgtttt cgtaggcggc gagttcctgt
    26281 tccacgatgt catccaaacc gatgttctta tgcctgtgaa taaccagatg gttgcgggct
    26341 tcctgattca gcaggggtga ttcgtaatca gggtaaccga atttgctctg gaatgcatcg
    26401 agtgcagagt gtgaagcgca gggagagtaa acctctccct gcaggatttc atttaagcga
    26461 tcggcaataa actgctcacg gctgagaata tccgccagca tatccttgac cgataatcca
    26521 tccacgacgc ccttcattgt catgcggcgc gaaaaaccaa cctgattcag gagcaattcg
    26581 aacttatcgc gttcgcgttg caagcgtgga agaaaaagac tcttgttcaa tctagtcacc
    26641 cgccaatggc gattgctcac tgatgaccga caattgattg ctggtcaccc gtacggggct
    26701 cttaacttca ggacacttcc agccttcccc tgggttttcg cgtcgcaggt tggcaaaggt
    26761 cggcaaaatg ccacaggcaa aacaattcaa gcggcaatcc acgcggatct gcccttcgag
    26821 cgattggcgg aaatcttgaa agaggaaatt cttgcgcacg gcggcggtaa tgtgttccca
    26881 cgggaagacc tcgtcggtgc ggcgctgacg gtgggtataa aacgcggggt cgagcccgtg
    26941 tgcttcgaaa gcaggcagcc aggcttcctg tttcttggct tcatcccagg catcgaattt
    27001 cgcgccgttc tgccaggcgg tgtagatcac ctctgccatg cggcggtcac cgcgcgaaag
    27061 ccaggcttcg agcagagagt cttcgggttt tgtccagctc aacctgatgc ttttatcttt
    27121 caaaagctgg cgtttcagca gggcttgttt ttcgaggatg ttttctttgg tatcgcaaga
    27181 aacccactgg aaaggcgttt gcggtttggg cacaaaggtg ctcacacctg catgcagttt
    27241 caccttccag cctgccactt tacggccttc ttcaatgacg cggcggcaca gatctacaat
    27301 ggcttgcaca tcttccacgg tttcgctggg gtggccgatc atgaaataca gtttgatcgt
    27361 cgtccaaccg cggctgtaga tttcgcgggt agtgttgatg atgtcttcat cgggaatgaa
    27421 cttgttgatg atgcggcgca tccgttcgct ggcggcttcg ggcgcaaggg tgaaacctgc
    27481 cgaacgatgc tgtttcagct tgtccatcag atccacggag accgattcga ttcttagcga
    27541 cggcaaattc accgagagtt tgcgcccttc gaagcgtttg ctgatggcat ccaccaattc
    27601 gctgatgtga gtgtaatcgg atgacgagag cgacatcaat gcgagttctt caaaccccgt
    27661 agagcggact gccgcatcgg cggcatccac cacctcttga acgcttcttt ctctaacggg
    27721 acgcgtgatc atacccgcct gacagaatcg acaccctcgg gtgcatcccc gcatgatttc
    27781 caccgaaaca cggttgtgaa tcacttcaat gttcggcaca atgaatttaa cgggcggcgg
    27841 gagcaacttt gccacaatac gcttgtttac cagctttggc gcttcgggta tggtgggcag
    27901 ggtgtgcgcc acagttccat cttcaagata ggtggtctcg tagaattttg gcacatacac
    27961 acctgagatc tgggcaagcg ccaccagcaa ttcactccgc ttgtagcttg gagacgattt
    28021 gaacttttga attgtgtcaa caacatcgtg tatgacttct tcaccctcac cgatgacaaa
    28081 tgcatcaata aaggcgtgca tcggctcggg gttggtggtg gaatgtccgc ctgcgatgat
    28141 gatggggtgg ctttcgttgc ggtctgctga acggacggga atgcctgcaa ggtcgagaat
    28201 gttcagggtg tttgtgtaga gggtttcgta agggagcgta aagccgatga ggtcgaaaca
    28261 ggccaggggt tgtttggatt cgagcgtgta gagcgggatc ccgtgttcac gcatcaattc
    28321 ttccatgtcg gtccacggtg cataggcacg ttctgccaat gcgtcttcac gttgattgat
    28381 ctgatcgtaa aggatcttaa tgccgacatt tgaaatgccg atgtcgtaaa tatcagggaa
    28441 gacgaaggca acctttgttt tggctttttc ccaatctttg acgatgctat taagctcgcc
    28501 gcccacgtat cgaccgggct tttgtacctt taaaaggatt cggtcaagtt ttaattcgat
    28561 ctgttctggg ttcatcatgg gcgggattat aacaaagaac ctccgattaa aaagaaagcg
    28621 gatggacttg tgagggtcca cccgcttttg aggggaagat gaaattaagc ggcggttttg
    28681 cgggcttgca tggtttcgcg gccccaaagc cagagggcgc caaggccaag taaaacaacg
    28741 agcaggttag ccagccagcc aatgcctggc aacgcggtca acagagcgac gatgatcacg
    28801 ccgagcgcaa gcggccatac tttactttcg gccagtgcag ggttgatgcg cgcaaggatc
    28861 agtttgccgc ccagccagga aatgacgatc ttggtaagaa aggcggttgc cagaacaaag
    28921 ccaacgatca atgcaaagat gatgagcaga ccaaccacaa tgatcgtgcc gctcaggctg
    28981 ccaagttgga gtgcgccgaa gaggattccg ccaataacca tggccacgac aacgatgaag
    29041 atggcgaaga agaagaccgc gtaggcaacc acgccccagc ccagtgatat ggctgcctgg
    29101 gtttggactt tttctaccag ggtcttgatg aaagttggag caagccagcc tagcagcaat
    29161 ccaaggatca ggagggtcac gatggtgcgt aacaggttga ggctccaatc cacgatgagt
    29221 tcagattgcg tgggtggggt aatggtttct tctgggtcga taacgggttc ggtgcgtgta
    29281 acctttccgc cgacaacatc ggtcggaata tctatgtcgg cgaattgggt gtaatcaaag
    29341 ttaccgttga tctttgcacc ttcagcaatg gtaatgccag ggttgacggt aggcatggaa
    29401 aacggtacct gtggcatgaa catggaaaat ggaggaccag cttcttcagt gggctcaccg
    29461 acgttggcga gtacgttgcc gccaaattct ccgcgcaagt caagcgctcc caagccgagc
    29521 agtgcatcgc cagtgacatt gcctgccagc agggcttgtg cggcaccgat caccaactca
    29581 ccttcgatgg aactgccttc tttggtctca aagctggcac taccgccaac cacatctccg
    29641 ccgattacgg ctgagtcgct gataagaagt gcggcacctg cgatacgggc gtcatctgaa
    29701 accgagccgt tgatcgccac gctttgcccg gcggcgatca aatcgccttc aacaacgcca
    29761 ttaatggtga tattggttcc aaataccacc aaatcacctt tgactgtccc ttccagtatg
    29821 aaagtttcag cgttgacata tacatcgtct tcgatcactt catctttctc gatgacgact
    29881 ttattgcctg ttcggccatc aaatgcctgg gcaggggtgg cgaaagcaag ggcaaggatg
    29941 gctatgaggg aaaaaatgga aaaaagttta tgaatggttt tcatgggggc tcctttgaaa
    30001 aaaacttccc gatgtaggta tacgtggcca tcatcgggag gttgcattca ttttattcgg
    30061 ttctttggag ggttattttc gtcctaaaac ggcgggtgat gccttctttg aggagtttcg
    30121 ccgcatccac atgacctgat tcgactgcca tgtcgatggg gagttttccc tccctgctgc
    30181 gaatcgtcaa atctgcaccg ttgaagagga gcgagcggat tatggcgaca tcgccatttt
    30241 gtgcggcggc atgcagcggc gtgtagccac cttgttcgcg tacatttggg tctgcgcggt
    30301 ttttgaggag gaggagcacg atctcaacat ggcctgcggc ggcggctgat tgaattgggg
    30361 ttgcccttaa ttgatttcta gagggagagt ttatggaagc cccagccttg atcaggtatt
    30421 cggcggtttc ggcatggcca aaaaaacatg ccaacccaag cggttgaaaa ccatcatccg
    30481 tataggcgtt gacgagcatt gggtcgcggg caaggtggcg caccacctgt gtggttctgc
    30541 cgagggccga agcttcgaag atattcaggc tgcccgtttt ttcggcgagg taattcacga
    30601 tcttcggttc gcgatgatag agggccacca tgaccgggct gagaccattt tctttttcat
    30661 agatcaggtc ggggtttgat gaaaggaggc gctggaccac ttcaaggtac ccgcgcttga
    30721 tcacgtcaaa aaattccttg ttcatttgat gatcactcca tttcctctgt aggatttacc
    30781 cgcatgtgga taattaatga ccgattatac gtctgatggt ttgtgaagct gtttgcaatc
    30841 ctgttagccg tttttgagtt attgaaggaa gtattcagcg atttttagag cgatgcgtgg
    30901 atcgtctgga tagtcataat cacggttaag taaaacaatg accgctaaat ggcgttcggg
    30961 aaaacggatg taatcggagt tgaacccgag ccaggcaccc gaatgagcga cgtaattttc
    31021 accattccac tcttccagtt cccagccgaa gccgtaaggt tcgatgctcc catcgtttaa
    31081 ttctgtgggt agaaatgctt cggccagcag tgcttgcgag acgaattttt cggtgtaaag
    31141 ggcttcatcg tacaaagcca tatctgttgt tgtggaataa atggagcctg aaccatagag
    31201 attatccaaa tcgtcgtagg ggtaggcctc gggtgttcca ctttcattgg tatagctcat
    31261 tgcaaccagt gggtcggcgc ggcgtttttc gtttggcagg gagaaggtgt gggtcatgcc
    31321 ggcagggtca aagatatgag attgcataaa gtcagggaag ttttccccgg aaacgcgttc
    31381 gatgaccact gccagcaggt cgtaaccggg attgctgtat tcaaatgaat ctcccggggt
    31441 ggagggtaat tcggtcattt ctgagagagc ggcgacaagg tcttcatttg ttggggtatc
    31501 tgaacgggtg taaagcgcat cggtaatttg gtcatcgtaa tcgggcaggc ccgaagtatg
    31561 gtggagaaga tggcggatgg tgaactcatt gccaaaatga gctacttcgg gaacgtgaat
    31621 gcctaccggg tcatccaggt tcaagctgcc atcttcaaca agcatgagaa tgccgagggc
    31681 tgtcatttgt ttgccaacgg atgcgagatg aaagatgtgg tctgtggtga gcggtgtgcc
    31741 ttcctccaga ttggccaatc cataggcggc gtgatgaagg attttcccgt tttgcacaac
    31801 gattacggca ccgcctgggg tgttgtcact gacaaactct gcgaagaatt catccacttg
    31861 ttggttgggt gtggtggaat ttgattcaga tggcatggga acttccattt ctgtggtgcc
    31921 gcagccagtc aggaagagca gggtgaaagc gaggaaggct ttgaaggttt tcatgaccac
    31981 gccttgagaa tagtcatcgc ggcggggtgc ccccaatacc agtgcagaat ttcgacaaac
    32041 cagccgcata gggtttgttt ttcattttcc aacacggatc gtaattcgtc catgctgagg
    32101 tagcggattt cggcgatttc gacagggtca aatttcacac tggcggggtc aacgaggcct
    32161 ttgtagaggg tgctgatctc gttgtcgttg gggccgtaga tcattttgaa cttcacgagg
    32221 ggggtgagtt cttcgaccgt gatgcccatt tcttcccgca gcccgcgtcg ggcggcttcg
    32281 agatagcttt cacctgcttt gacatgctcg gagacggagc aatccagcag agatggggcg
    32341 gaggtgcggt cggcgctgcg tttttggatg agcattttgc cttcgggtgt gaaaaggaaa
    32401 acatgcgcac cgcgatgttg taatccattt tggtggatgg ttgagcgagg ggcttgccct
    32461 gtgacaatgt cattgtcgtt tacgatgtcg agggattctt ctgtcatgtt tttttgaggg
    32521 gagaaaatat caatggtggt ggagcaggtt gagggtgagt tggaggcggg gtttgatttg
    32581 gaaaaagaaa gaggcgcgga agagcgatcc gcgcctgctt gttttgtggg ctatccaaac
    32641 agttcaatga gttgtttggc gtgtacgatc ccgtcaaagt ctttgatgtc gcctttgcgc
    32701 attttcgcct tttcgccgtc ggggtcgccg aggcaggaga cgatgacatc gcctgcttcc
    32761 acatcttctt tttcggtgta gggattggtg gtgacgatgg ttttcaggcc agcggctttg
    32821 gcggctttga caccgttctt ggagtcttcg accacaaagg cttcttcggg tttcaactca
    32881 agtttggcaa ggccgaggtt gtagatttct ggatcgggtt tcttcttttc aaccacatcg
    32941 cctgcgagca caacttcaaa tttgatgtcg gacaggatgc cttctgtgat ggctttggcg
    33001 gcctgttcat tggaggtggt gcagaccccg atcttgatgc cagcttccat gagttctttc
    33061 atgaagcggt gaatgccggg gcgcagggga agtttgcccg attcgatcaa ctcgacgaaa
    33121 agggcggtct tgcgtttgtg catttccttg acgagattat caatctcttc ttcggtgagc
    33181 ggcttggaaa accctttggt cttccagtga tgtttcatgc gttccttgcc gccagcgatc
    33241 tggagtaatt cgtggtagta ctccacgctc cactcgtcgg tgaagccgaa ttccttgaag
    33301 gtcatattaa acgatacgcg gtgaccatcg cgttcagtgt cgatgatcac gccatcctgg
    33361 tcgaagaaaa ctgcttttat tttggacatg gtttcctctt tctcgccaca aaggagcgcg
    33421 aaggatcaca aagttgtttt tctctgtgat ccttcgtgtt cttcgtggaa accttatttc
    33481 ttcattccaa agaattcaag cacggtgttg acgaagagtt cgttctcttc cttcgagccg
    33541 atggtcacac ggaaccagcc atcggaacca tatttcttgc tttcgccgcg gaggattatg
    33601 cccttggttt cagcgtattc gagcacctgt ttgccggtct tgccggtttc gccgcagtcg
    33661 aagaggatgt agttggcttt ggactgcatg acgtagaagc cgggaatgac gctcaacgat
    33721 tcgctaatgt aatccaccgc gtcgttgttg aacttgacgc gctcggcaag gccttcagtg
    33781 tcttcgaacg cagcaagggc ggcccacatg gggatggtac ccacgttcca aggcaggagc
    33841 gaaccggcaa cctgtttaag ggtgtcttcg tgcgcaatgg tgtaaccgaa acgcatgcca
    33901 gccaaaccat aggccttgga aagcgtgcgg gtgatgaaca cgttcttgta cttcttggtc
    33961 aattccactt gcgacttgcc caaaccagca tattcgatgt aggcttcatc cacaaccaac
    34021 ggaatgccta acttggcgaa gggttcaaag tgctccggat ccatgaagtt gccgctgggg
    34081 ttgttgggat tcgggatgac gatgatcttg gtcttgtcgg tgacggcttt catcaaacca
    34141 tcggggtcga atttcagttt gtgatcttcg tagatcatcg gcacagagac catcttggcg
    34201 ccgagcaatg ttccgcgcag gccgtaaatg ccgaaacagg gggtgtgctg aatgacttcg
    34261 tcgcccggtt cgatgaacat gcggaagatg ttgtcgtaca cttcgctgga accgttaccg
    34321 a
//
LOCUS       DAOQJC010000033        34204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_557_length_34204_cov_10.736654, whole genome shotgun sequence.
ACCESSION   DAOQJC010000033 DAOQJC010000000
VERSION     DAOQJC010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 34204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34204
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_557_length_34204_cov_10.736654"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PLE39_06790"
     CDS             <1..789
                     /locus_tag="PLE39_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68069.1"
                     /translation="KTPALQNVNLQVNEGEFVAVVGPNGAGKSTLCYTLAGFAPHFFK
                     GEMSGTVEVAGKESSKSTLDEWVLNVGLAFQNPFNQISGAKYTVFEEIAFGLENIGVP
                     RDEMKNRVEDAMALTGIRDLADRSPYSLSGGQQQRVALTSILVMRPKLLVLDEPTSQM
                     DPIGTREVFGTIRKMAEEGMTVVLVEHKVEWIAEFADRVVALKDGSVLLEGKPNEVLT
                     SELLAEHGFGLSRYTSTAREARKRGLWKKENLPVTLDEAVEGFS"
     gene            825..1616
                     /locus_tag="PLE39_06795"
     CDS             825..1616
                     /locus_tag="PLE39_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68070.1"
                     /translation="MKIEIKDLRFIYPTGLEALKGISLAIESGEQVAIVGQNGAGKTT
                     LVRHFNGLLQPASGSVLIGDWDVKKHSVAKLASRVGYVFQNPDEQLFSRDVLTEVTFG
                     PKNLGYAREKIDQLVKRALALTELNDKTETNPYDLSPTWRKMVALASIVAMDTPIVIF
                     DEPTTGQDAVNVARIANVIAELRKEGKTVITITHDIDFCAENFERVIALSKGQVLLDG
                     PAREILGQEEILAQTYVDPPQLTRLGKRLGLKETVRNQEEFLNSL"
     gene            complement(1679..2077)
                     /locus_tag="PLE39_06800"
     CDS             complement(1679..2077)
                     /locus_tag="PLE39_06800"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNE68071.1"
                     /translation="MKAHTFRLKPAQDLFDSIEEFVKQNNIQAGCVLSSVGSLTHATL
                     RLANRSHYNEYEGHFEIVSMTGTVSIHGSHIHVAISDGDGVTIGGHLVSGCKIYTTAE
                     IVIAEFEDVVYKRELFENDSGYEELVVYKT"
     gene            2104..2814
                     /locus_tag="PLE39_06805"
     CDS             2104..2814
                     /locus_tag="PLE39_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNE68072.1"
                     /translation="MKSYRYFDIILGVFIAVLLISNVASSAKIVDFGFGIFGIPLAFD
                     AGTILFPIGYVFGDILTEVYGYQRSRRVIWTGFFSLALAAFVFWIVGRLPGEVTWQGY
                     AGDAAYQSILGGMSSGGILFASLAGFWLGEFSNSFILAKMKVWTNGRWLWMRTIGSTL
                     VGEMMDTAIFVVVATAFGVFPWSLFLTLTVTNYLFKVMVEVVMTPLTYIFVRALKRAE
                     NEDYYDRETNFNPFAIIK"
     gene            complement(2844..3404)
                     /locus_tag="PLE39_06810"
     CDS             complement(2844..3404)
                     /locus_tag="PLE39_06810"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNE68073.1"
                     /translation="MSFRSLLELDARLSGQMRVAEKPGFLRNVAAFFAHSGDSWFWFA
                     ALLLFWFFSNSFWKHWEVVEFFGILGLAGVVLGIKFLVRRKRPEGEWGGIYRNTDPHS
                     FPSGHAARSFLIAVLASFLAPPWLAAVLWVWAPLVALARVAMGVHYLSDVIAGAILGV
                     IVALIGMQIYQPMIDWFTALTHFSFW"
     gene            complement(3401..4309)
                     /locus_tag="PLE39_06815"
     CDS             complement(3401..4309)
                     /locus_tag="PLE39_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNE68074.1"
                     /translation="MDAIVTAGGIPQPGDPLYVYSNGNSKALIDISGKPMIQWVLDAL
                     GESKKVDNVIVIGLSPKSGLTCKKPLHYISNQGRMLANIVAGVNKSLELNKKNEYVLI
                     VSSDIPALKGDMVDWLVDTCMSTKDDLYYGVCPREVMEERYPTSKRTYTKLKEMELCG
                     ADINVIHVSMATDHLNTWEQLIGNRKSPLRQAAVIGWDTLFQLATRQFTLQGLVERAS
                     QRIGIKGRAIVWDKAEPCMDVDKPHQLELMREDIASQLQKAAKAEKKKVAAAKKAAEP
                     VSKTKKTTASAAAKPKPKPKAKVSTK"
     gene            complement(4312..5217)
                     /locus_tag="PLE39_06820"
     CDS             complement(4312..5217)
                     /locus_tag="PLE39_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68075.1"
                     /translation="MKRAVSISIGSSKRNKAVEVTLLGEKVSIERIGTDGDMEAAALK
                     YQELDGTVDAFGVGGADLGAIVEGKFYPFHSVQKLIRYIKKTPVVDGGGLKNTLENKA
                     PGFLDKKIGDYINQRGRKVMVTVGVDRWGLSKSFAEANYETVFCDLMFALDVPIPVRT
                     MKGLRTLAAILIPIVTRFPFEWLYPTGDKQNEHKPKYENWYQWATVIAGDCLYIKRHM
                     PLNMQGKVVVTNTTTPEDVEFFRKCGIKYLITTTPVMDGRSFGTNMMEAALVAISGKG
                     RPLSFDELNELLDKLGFEPQLQELN"
     gene            complement(5296..7893)
                     /locus_tag="PLE39_06825"
     CDS             complement(5296..7893)
                     /locus_tag="PLE39_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE68076.1"
                     /translation="MPNRFRRLLSSPIFENEEKTRTAQILNSFVWSAISILLIIIIIR
                     AIIGQEENPMPLLVLTAIVVTLGIVQVIIRRGYIRGASIFLAGSFWVIITFQAWAADG
                     IRDAAVISYILIILLSALILGWREGLFFGVISLSALWFFAVEEEWGLRVQHMDTPLNL
                     ARDFTVIFLFAGTLIYLLINSLNRTLYDARLELKERLRAERKLQVQADYLTALNETAL
                     GLLNRSELRPLLESILSRACALLDTQHGLIDLVLPDESALRQELGHGVLSRYDGSLTQ
                     KNVGVTGEVWGSGKTLVIQNYGEWEKNLPEFVDAGFNAVMGVPLKVGNKVIGVLAISF
                     VEQERTFTHDHVNLMERFAALASLAIDNARLFEQTQKEIAERTMAEAALRSSEERFRK
                     VFHASPVAIVITSLSEGTIIEANEAYWRLSGLDLQKTIGYQATELGLWQDEEEPGDFI
                     ERLKAERSIYVPEYLFLNEREGLYSTVLAFYELVKLEGTDSILSMFHDITEQKNARES
                     LQNSEARTRAILASIPDMIFEISKDGVFLDFMASSQLVPLMSPSQFLGRTVQELFPPI
                     IAQQTLFALERTLATGHLHAFEYGMPPGEEVQFFEARITAISPESAIIMVRDISQQKY
                     VTMEREKLINELEDKNSELERFTYTVSHDLKSPLITIKGFLGFLEQDALSGNTTRLKL
                     DIQRIGDATDKMQLLLSELLELSRIGRLTSPYHFIKVEDLITEAVEALHGRLNKAHVK
                     VETQGDLPTIYGDHQRLLEVFQNLIDNAAKFTEGQPDPHIEIGQAGLENEQPIIYIRD
                     NGIGIDPIHHDRIFGLFNKLDASSEGTGIGLALVKRIIEVHGGRIWVQSEAGHGSTFF
                     FTFPNGPES"
     gene            8134..8227
                     /locus_tag="PLE39_06830"
     tRNA            8134..8227
                     /locus_tag="PLE39_06830"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8168..8170,aa:Sec,seq:tca)
     gene            8310..9488
                     /locus_tag="PLE39_06835"
     CDS             8310..9488
                     /locus_tag="PLE39_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNE68077.1"
                     /translation="MSIPETEILTNLVSRVFRIEDVTSEDPRKGFFLRYRGVLANEDS
                     AALYDTLADSLSPYGIIPLFRMEEGRQVVYLAPKPPEPKQDRISTNILLFVLTVFSVM
                     LAGAQPEGTMPDDFMGQLLMLAKSIFTGWPFALSLLGILLTHELGHYFMSRYHKTPAT
                     LPYFIPLPLSLLGTMGAAIIMRGTPKNKRVLFDIGVAGPIAGLIVALPVLFYGLSLST
                     LGTIDPNPNGFIEGNSLMYLLAKYITFGQLLPSPLEPQGILYWLQYFFTGRPVPFGGL
                     DVFIHPVAFAGWAGLLVTGLNLIPAGTLDGGHVIYGLFGEKARKGFPFIIGILAILGI
                     FWSGWWLWALLLFWLGRVNAEPLDQITTLDPPRRLLGYIMILVFLLVFMPVPFMTLAQ
                     "
     gene            9496..10872
                     /locus_tag="PLE39_06840"
     CDS             9496..10872
                     /locus_tag="PLE39_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNE68078.1"
                     /translation="MKKIILSFIIFTFVLAACGTQEDVTSTAEPAVTQAATKTPQPEP
                     TVEAASRLGVGEEALNGLEITVWHPWYGVEASLFESFVEDFNESNSWGIKVVAESQVS
                     FTNLYENLMASIPGKDRPDLAIALPEHALEWDNNGMSVDLMPYVNDPAYGLDSSNIPV
                     VFWNQDLDGEKRVALPAQRTARFLLWNETWADEIGFASPPDAPDDFRQQACRAQQFML
                     KDESPQNDFKGGWYADTDYMTAYAWLLAFEGGVLEGSDYRFLTPNNIEAFKFLRELSE
                     STCSWQSADTDAVTAFANREALFITASLEDLPDAARAFASAGNTDTWKVLSFPGENED
                     ALVVYGSSYVMLKSTNEEQLAAWLFARWLTDEKQDARWVEATHLFPLRTTTLDLLADY
                     KKTHPQWEQAVKLLPQGEIQPQLDSWRKVKIMLGDGFTHMYRVNVPSGQVAAILAQME
                     ALSRELSR"
     gene            11079..11870
                     /locus_tag="PLE39_06845"
     CDS             11079..11870
                     /locus_tag="PLE39_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68079.1"
                     /translation="MPESEYRARQVRAKTNEVFEYDRDDEKPGKPLHILIPGGLIALA
                     TLALIVFIAYQTWFEGNLAENIGLILVVILSPFYVGGVFLFSYGYELYNIPRALRLTA
                     IIVFVTLAAVVIVAVLFFLLANMNGSSGSKSSSSNSSKGGKSRSSSSSSSSKPSNSPR
                     PSTGKMGGIVGAAGAALNRSSTNSSSSDSSSSSGYSSGGHVYIGGGETREVTKEVVKE
                     VPKEPMPVTCPFCARSYVPVENNYICPSCGAATPRELIKESQEPK"
     assembly_gap    11948..12047
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12048..12366
                     /locus_tag="PLE39_06850"
     CDS             <12048..12366
                     /locus_tag="PLE39_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68080.1"
                     /translation="KAIKKTKNNDGRSEYDFSKAQRGRFYRPLDKGYTVHVRKSDGTE
                     VVNHYTLKEGTVLLAPDVLEYFPDSESVNEALRSLIHLMENVPVKKYSVSKMATHQVA
                     ERK"
     gene            12443..13906
                     /locus_tag="PLE39_06855"
     CDS             12443..13906
                     /locus_tag="PLE39_06855"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE68081.1"
                     /translation="MRQMLINGKFTNGNATEEIEVQNPATEEVIESVPRGTPQDVEDA
                     VQAAKSAFDSWRKLGSNERARLLHEVADRVHEHREKIVHMLTLEEGKPVSENEEEVEW
                     VINTFHYYAELGRHHRGSVLASGEASQFNFIIKEPYGVVGCIVPWNYPLLLLAWKVAP
                     ALAAGNTVVIKPSEMTPLTALYLAKHCFNGLPAGVVNVVTGYGLETGEPLVKHPDVPV
                     IAFTGSLATGQRIASIAAPMMKKLHLELGGKDATVIAEDADPEIAAKAVAYAALINAG
                     QVCTSTERVYLPKTHAAKFTEAIVEHVKSLKLGPGLDPKTDMGPMIGETFRAKFEKQL
                     VDARASGAKILTGGARPQYLEKGFFHEPTVLTGVNHSMVIMREETFGPAVPIMEYTSF
                     DEAIQLTNDCQYGLGAVLISNDPKKIKRFFDDVKAGTIWINDPLTDNYAGPFGGMKYS
                     GGARELGEEGLEEFRETKHVHWDFNMEEKGYWFPYGR"
     gene            13957..15141
                     /locus_tag="PLE39_06860"
     CDS             13957..15141
                     /locus_tag="PLE39_06860"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HNE68082.1"
                     /translation="MRKQSWLFPFVLLLAVILYFQISRVQLPGDGFSTFGSDTVRAEV
                     IQIIEEGEVDLGGHTQKYQIARVNILEGEYEGIPMEIDYGKRQLRSDDYDLVVGGRVM
                     VSISKTPDNVVNAYFVDFVRTSPLLWLTVIFAAAIILISRWKGVRALLSMAFSLYVII
                     DQIIPHILNGDDPLWVSIIGSIVLLSVTLYLTYGWNLKTHASVLSMVLVLLLTGALSA
                     VFVIFTKLNGTGDENVMFLMQMMEKPLNLRGLLLGGMIIGALGVLDDLVTTQSAAVFE
                     LHHANPSFKFRDLYNSAMRIGQDHVAATVNTLVLAYAGASLPMLLMFSLGHGNYGYII
                     NFSFIAEEVVRTLVGSLGLIAAVPLTTAIAILFAQRAELLGRWEQVLGPEGSGDGHGH
                     AH"
     gene            15255..15857
                     /locus_tag="PLE39_06865"
     CDS             15255..15857
                     /locus_tag="PLE39_06865"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE68083.1"
                     /translation="MNEEQTWVIQAQQGSDEAFTHLVETYQTPVFNLCYRMLGEPDLA
                     EDAAQETFLRAFQHLHRYDQKRSFATWLLSIAAHYCIDRLRRRKFTIFSMDTEDEDGN
                     TFELPDLDSPNPEAESIKGQTRDRIQAMLKDLDPTDRAAIVMRYWYDYSEVEIADSLR
                     LTVSAVKSRLHRARKELAGLWQEEETRAETERRPYESPAF"
     gene            15838..16368
                     /locus_tag="PLE39_06870"
     CDS             15838..16368
                     /locus_tag="PLE39_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68084.1"
                     /translation="MNHQPFEEWLLNDKNLNSAEKRELDLHLRTCAHCTALSATGLAL
                     RSANVVAPASGFALRFQQRLAAQRIAERRRKLWGAMVLVLSGFALLTWFVAPFVYAFL
                     NSPAEWITAAVSYFLFFASSVQALTEAAAVLFRILPDFVPPYMWMVFVSALAGLGLLW
                     TISIWQFSRTPQGVSA"
     gene            16365..17450
                     /locus_tag="PLE39_06875"
     CDS             16365..17450
                     /locus_tag="PLE39_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNE68085.1"
                     /translation="MKSKLTTLFLLLALLFLPTGAVHAQSPDGGDVFLLGQNYTLKSD
                     ETLDGSLVVIGGNVDIEKDATVNGDSVLIGGNLQVNGDANGSVVLIGGNLVISGKIKG
                     DIVLIGGQALLTETAVVDGDIVTMGGQVQKDPGAEVTGQIVNNAPPIDVPDIPDVPNV
                     PDAPDAPDAPDIPDVPGVPQINVDFNPLWEVTGVFTRALAIAAVGMLLTLFLQPQLDR
                     VADAITRQPMMAGSFGLLTVVVAPVAILIMIVTILLIPVAAIVAFILLPMAWLFGMVA
                     IGQEIGDRFTKAINQTWAPVLSTGFGTFLFLLVGGFIGLIPCIGWLISLMFSLVALGG
                     VMMTWFGTRAAPGSAPAVVTETIPPAS"
     gene            17671..18783
                     /locus_tag="PLE39_06880"
     CDS             17671..18783
                     /locus_tag="PLE39_06880"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNE68086.1"
                     /translation="MFTKQEIPNTPIHNTPPPLIEVSGTHREMGRQIGEAARAQVQHS
                     VANARVLIDAAYDSLELTWQGAQIQSRKYLPFAEERYPQYVDEMRGIAEGANVAFDDI
                     VTLNAMEAVTVDALHLTRCTSMAVNEERTADGHVLAAHNEDWVPEDEDGVLVISAKPD
                     KEPPFLAMTYGGLLPNVGFNAFGIAQLIDSVYPSDSRIGIPRLVVARAVLSSHRISGA
                     IGRTLVQHRAAGYNHLLIHESGEMYSIEVSARKFEILYAHDGYMVHTNHYLDPQMKQI
                     EKDPEELLSSRVRYFRASRLLRQNGQHTIKSLQAIQKDHTNIPNSICNHNIEGLDPLD
                     REKTISALVIDLTSREMHITWGNPCQNAYHTYHLNA"
     gene            18896..19768
                     /locus_tag="PLE39_06885"
     CDS             18896..19768
                     /locus_tag="PLE39_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNE68087.1"
                     /translation="MRDLTNEILELIRRTSSSLPPDVEKRLRESIEKEEPGSAARGAL
                     ETILKNIDLSRANSTPICQDTGTPIFYVHYPVGWSTIKLKEQIRSAMAEATKRSYMRP
                     NAVDAIYDKNTGNNLGGDDFPYVHFEETEGDDLTIELMLKGGGCENVGRQYSLPDNSV
                     GAGRDLAGVRKVVLDAVQKAQGQGCAPGILGVAIGGDRGSSYIKSKEVLFSEMGTRNK
                     DPKLAELEERLTSEANQMGIGPMGFGGKTTVLDTKITGLSRLPASYFVSVSYMCWAYR
                     RRKMTVKGEEITYD"
     gene            19814..>20295
                     /locus_tag="PLE39_06890"
     CDS             19814..>20295
                     /locus_tag="PLE39_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HNE68088.1"
                     /translation="MKQIKTPITDEVIRELKVGDAVSLSGMMVTGRDAAHKWMIETFI
                     KKTRAPQGDDLQVYEELKKLLAGSVIYHCGPVVTGLDTKEYKIIAAGPTTSIREEPYQ
                     ADVMKHFNVKGVIGKGGMGAKTLKGCQDTPGVYFHAIGGAASFLAQTVKKVHGVFKLD
                     "
     assembly_gap    20296..20395
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20396..20721)
                     /locus_tag="PLE39_06895"
     CDS             complement(<20396..20721)
                     /locus_tag="PLE39_06895"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HNE68089.1"
                     /translation="MDSNEIQPSSPLNTKSILSLVFAVLTLLTFCTGWLPVPFTGILC
                     FPASFLLGILALTYGTISLRQIRQRNETGHPMAWTGVIIGGFVFVCVLCMLIAIISIF
                     LYSPDLL"
     gene            complement(20765..20839)
                     /locus_tag="PLE39_06900"
     tRNA            complement(20765..20839)
                     /locus_tag="PLE39_06900"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20802..20804),aa:Pro,seq:ggg)
     gene            20968..21546
                     /locus_tag="PLE39_06905"
     CDS             20968..21546
                     /locus_tag="PLE39_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNE68090.1"
                     /translation="MSDMIEVVIDSIRVHLMTPQRVVVLKQTNAERYLTIWVGPYEAE
                     AITVALQEVEMSRPLTHDLLKNIFTTFNARILRIEITELQGEVFFGNIIAEVDGRELR
                     IDSRPSDAIAIAVRAHVPILVHAHVMESAGMAPDQDMPDTSPTTPLARREAPAPLSDD
                     ANKRLSVFKDFFDKLDIDNPDRDKPDSTPPLD"
     gene            21556..22962
                     /gene="dnaB"
                     /locus_tag="PLE39_06910"
     CDS             21556..22962
                     /gene="dnaB"
                     /locus_tag="PLE39_06910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNE68091.1"
                     /translation="MTDSLPETFPPYGDDSSSAAPASSTSSSIPHSREAEEAVVGAVL
                     INPEIYYDVAQFLSPDDFYIHRNKFVWEAFTRLHEQRIPVDLLTLSEELDRVGQLAEI
                     GGSAYLTSLINQVPSSLNAESYGRIVEGHSIRRKMINAANKIASVAYNEASTVEDVMN
                     EAEKAVFNVSERRLKHDLQPISTVLSDYYDRIDDLAKRPDEIHGVPTGFIDLDRLLTG
                     LQPSDLLIIAGRPGQGKTGFLLSIAKNAALTHKKHVAIFSLEMSNEQVVQRLIAQETG
                     IDSQRLRNGKLHEHEWPLFTHAIEVFSDTHIYLDDTPAITPLQLRTKCRRLHMEYGID
                     LVIIDYLQLMGGDSRNDNRVQEVSHISRSLKVLARELNVPVLTAAQLSRAVEQRTDKK
                     PVLSDLRESGSLEQDADIVMFIYRPDQYEKDSEKQNVAEIIVAKHRNGPVGSVEMIFR
                     GAQAKFENAATRVFRPNE"
     gene            23035..23691
                     /locus_tag="PLE39_06915"
     CDS             23035..23691
                     /locus_tag="PLE39_06915"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HNE68092.1"
                     /translation="MAAFSGFTSSETFTQIPDSFIRLLGEIEDVAELKVALHAIWRIE
                     HMEGNFRALRESDFAVEMLGLEGREIKRGLKKCLERGTLLKAENESEVLYFLNSPRGR
                     LAVDSFTKGKLAGIASATRERSNLFKLYEENIGPLTPLVADVLKDAEEIYQAEWIAEA
                     IGLAVENNKRNWKYIEAILKRWKEEGRAEKQDRRNDKKDGQQSLSRKIEQLRKRADKK
                     "
     gene            23630..25003
                     /locus_tag="PLE39_06920"
     CDS             23630..25003
                     /locus_tag="PLE39_06920"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE68093.1"
                     /translation="MASRASAERSSSSASAPIKNDLPGDPNCPHCHGVGYLRSDVPVG
                     HAEFGKLEICTCRRSKVADNIRDRLFAMSHLDELKDLTFESFKPRGRKGLGEMQARSI
                     EMAFNHAQHYAKSLNGWLLLQGGYGSGKTHLAAAVANFVVGMGVPTLFLTVPDLLDAL
                     RFAYGSEDTTFEERFEQIRSAKLLVLDDFGTQNATGWAQEKLFQIINYRYINKLATVV
                     TTNLSLDEIEPRIRSRLSDPELVSPVKMSAPDYRRPMEDTSHPELSSLDLLSSRTFGS
                     FEDRAAEKLTPDEHKSLQKALKIAHSFSEKPKGWLVFEGTYGCGKTHLAAAIANYRAG
                     LGDPPLFVMVPDLLDHLRAAFSPNSGTSYDRRFDEVRTAGLLVLDDLGAQSATPWARE
                     KLHQLLNYRYNAELPTVITVAKENLDQGQIDERIITRMLDERLCEYIEIKAPAYQGKG
                     KRKIGRK"
     gene            25231..26082
                     /locus_tag="PLE39_06925"
     CDS             25231..26082
                     /locus_tag="PLE39_06925"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HNE68094.1"
                     /translation="MLPSYLLSLREGLEAALIIGIVLGALQKIRRTDLAPMLWYGTFA
                     AIGVSILTAILLTIFGLSLEGSAEQIYEGITMFLAAGLLTWMIFWMSQQARTLKAELE
                     ADVNKAAASRNGRALFWMAFVAVVREGVELAFFITAAFFAGNTENVGANTIQTLAGVV
                     LGLSTSILLGWSLFATAVRLDLRRFFQVTGFILILFAGGLVAHGIHEFNEVGWIPNII
                     EHVWDVNAIVDEKSVLGELLKTLFGYNGNPSLTEMIGYFVYLAVVAVFFQRSNTKKDV
                     MVAQPSA"
     gene            26198..26866
                     /locus_tag="PLE39_06930"
     CDS             26198..26866
                     /locus_tag="PLE39_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE68095.1"
                     /translation="MNTKYEAYEQPGITVDLAIFTVNNNKLKVMLVRRAEDPYKDFWS
                     LPGGFIKQGESLEEAAIRVLNEKTGVKNVYMEQLYTFGDPARDPRSRVITVTYFALIP
                     WAQLAQPASEKVADLAWSHIDRVPKLAFDHKMILDYAVKRLRAKASYSNIVYGLMPVQ
                     FRLSELQTMYEIIINDKLDKRNFRKRMLSTGLLQETGEKDLTGAHRPAMLYQFKKMEI
                     AFLD"
     gene            26874..27893
                     /locus_tag="PLE39_06935"
     CDS             26874..27893
                     /locus_tag="PLE39_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68096.1"
                     /translation="MKKQTLPLPEFFESARVGEIWKVDYASRAEQARQYARQHDLKPA
                     SAASEKISLLVIDAQNTFCMPNFELFVGGRSGHGAVDDNVRLCEFIYRNLGRLTHIIA
                     TMDTHMSQQIFHPIFFVDPEGGHPTPYTDIHLADLISGRWKFNAALAPRLNIAPEYGQ
                     QMMIHYAESLEKKGKYALTIWPYHAMLGGIGHALVPALEEAIFFHSIARFAQPDFEIK
                     GNKPFTEHYSVIGPEVLTGPMGETLGSHDTTFIQHLQEVDRLYIAGQAKSHCVAWTVS
                     DLLADIQDADPSLAEKVYLLEDCSSPVVVPGVVDHTEAANEAYIRFAQAGMKVVKSTD
                     EISSM"
     gene            27929..28480
                     /locus_tag="PLE39_06940"
     CDS             27929..28480
                     /locus_tag="PLE39_06940"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HNE68097.1"
                     /translation="MPNLTRLSKFVSVILRHRANDFDLHLDAQGFTDFAELKRIVIEK
                     STDVYSEEDWQKVLNGELDGKKRFEVKDGRIRAMYGHSKVDPVSYPKVEPPEILYHGT
                     TPQAEKNIRREGLRSMKRQYVHLSSNLERANSVGSRRTEHVTILVVRAREAAVAGVEF
                     YAPEPEHFLARSIPPNFIDFPQN"
     gene            28489..29832
                     /locus_tag="PLE39_06945"
     CDS             28489..29832
                     /locus_tag="PLE39_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNE68098.1"
                     /translation="MSIFDGKRLTNETFKLDIDRMRRGWYSDKYFENINRMLTALSKE
                     NYNYSGSFPILPNGISPEQIAVGDIEVEMQWFTRRMGKTTVVGVDKSLEMLRHCTGYF
                     DGDTFVDTSDRLEVWAVQDGSIVKFEGDPTKIQPVIKVRGRYRDFALLETPTLGILTR
                     SSRVATNVYETLVASRGKPVLFFPARFDVHEVQAADGYAYNIALLRFNKDHAQELGAF
                     VSTDAQGDWWGGAGGGTVAHAAIASFLGDTAEAMMQFAHILPARIPRIALVDFNNDSV
                     RDSLRVLDAMFAKYRELMDAGNREEAEKYKLYGVRLDTSGSLRDVSVQPLGDPALDLG
                     VNPRLVFNVRQGLDNAWESWTLPTSWKDAAREFCHNVKIVVSGGFNPEKIRKFERLEV
                     PVDIYAVGSYLFNNSNSTSTDYTADVVRVKIHGEWIEMAKMGRKVGENENLERVW"
     assembly_gap    30108..30207
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<30208..30686)
                     /locus_tag="PLE39_06950"
     CDS             complement(<30208..30686)
                     /locus_tag="PLE39_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68099.1"
                     /translation="MPQNKSMPDEHQIEKILSTIQPMPGEGFYSRMKTAPWQVKRTGK
                     TTTMKYKYAFLTLAMLLALTATVVFVPPVRAQVEEWFFVKFQSPNNSNLSFGFVSNEP
                     MKYQLMQPGYLPEIFKDINSASFGDVSELLYKVDDTFLIITQQPAADNDALPDGEAVT
                     "
     gene            complement(30697..31266)
                     /locus_tag="PLE39_06955"
     CDS             complement(30697..31266)
                     /locus_tag="PLE39_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE68100.1"
                     /translation="MTEQPVLQNIKRWLWQEQEYSITDADAFAKLYELHHLTVFRYVY
                     GLSGGPQQDAEDITAETFIRAWTTRHRFTGSKQAALGWILRIARNLIIDSSRRRRVRP
                     EEENIDLESLLDTKSLPEIDIITREQAMVLWKTLSTLPEDLREMLVLRYMLGWQVKQI
                     ALHLDITENNVSVSMRRALNRLQQGWPQS"
     gene            31972..33603
                     /locus_tag="PLE39_06960"
     CDS             31972..33603
                     /locus_tag="PLE39_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE68101.1"
                     /translation="MLLQQIKPAKSFISTRVGWLMLALSILLIAGLSFFPMFNGEKDI
                     PSPTAAQTDIPPTPTPLFAPVEPVEPGQVMVLVANMEQVGSTKRDVTRFIVEDLVNHF
                     ESDLSSTNIRIREYERFITSNDEALQLAEQTQAVIVVWGQYDNDNVTVNIQLGSLQSR
                     PAIALDRSLLEQAVNIRVKLTDERQETLAYHILSSLILIHCAENDMVKAMSLIISFDL
                     LDAPGPEPIGNSSAAHVQKAMKAFLSDRKTALDELNQTVELAPDNPLFYMLRAILYQG
                     MGEFALSNQDLDTALLLAPEDWILPYHLKGGISLLLSDVPNGVEFYTRVIEKTPDSWF
                     PYNQRGYLYFLAGQYDLARSDIEKSIALKPEAEWPYMWGALIALRQGRMEDASFMMSL
                     LLQEQPNPTFINRLMTILYGEATAKLLGESMAAMGHLTLGQYDVAIQDTDGVLAVMPA
                     YTDMYMLRGLSYCNLDNYVDAEAAYTQGLTLDPSFALLHLLRVEVRTKQGNVAGAMED
                     IDALQQNNELTANLAPYLAAAQSGEFSCKQLLRSK"
     gene            33756..>34204
                     /locus_tag="PLE39_06965"
     CDS             33756..>34204
                     /locus_tag="PLE39_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68102.1"
                     /translation="MNDQLSKNVIKKIKADYNLEEIPRVAEYLGQYGTDKPETERVQL
                     GILNVANGDIPKLQMLVSVALTDYRDLLVQAEYLNDPYNLLQTLLRNLQESNTFTTEE
                     VTETMRATGGTNYRKAFEFLMVMIPQRGKTLNAEQYQLIDKLGRALKL"
BASE COUNT         8129 a         8340 c         8987 g         8448 t
ORIGIN      
        1 aaaacgcccg cgttgcaaaa tgtcaacttg caggtgaacg aaggcgaatt cgtcgcggtc
       61 gttgggccga atggcgcggg caagtccacg ctttgttata cgctggcagg ttttgcgccg
      121 cattttttca aaggcgaaat gagcggcacc gtggaagtgg ctggcaaaga atccagcaag
      181 tccacgcttg atgagtgggt cttgaatgtc ggcctcgcgt ttcagaatcc cttcaaccaa
      241 atttcgggcg cgaaatacac tgtctttgaa gaaattgcat tcggtctcga aaacatcggc
      301 gtgccgcgcg atgaaatgaa aaatcgtgtg gaggatgcca tggccctgac cggtatccgc
      361 gatttggcag atcgctcgcc ttattcactc tccggcggac aacaacaacg cgtcgctctg
      421 acctcgatcc tggtcatgcg gcccaagctg ctcgtcctcg acgaaccgac ctcgcaaatg
      481 gacccgatcg gtacccgcga agtcttcggt acgatccgca aaatggccga agaaggcatg
      541 accgtcgtcc tcgtggaaca caaagtggaa tggatcgcag aattcgcaga ccgagtcgtt
      601 gcgctcaaag atggcagtgt gctgctcgaa ggcaaaccga acgaagtgct cacctctgaa
      661 ttgctcgccg aacatggttt cgggctttca cgctacacgt ccacggcgcg tgaggccaga
      721 aaacgcggac tttggaaaaa agaaaatctg cccgtcacat tggatgaggc tgtggaaggg
      781 ttttcgtaag ggcacagcgc cgctgtgccc caacgggata atcaatgaaa atcgaaatca
      841 aagaccttcg ttttatctac cccacaggcc tcgaagcctt gaaaggcatt tcactcgcca
      901 ttgaatcggg tgagcaggtt gccattgtcg gtcaaaatgg cgcggggaaa accaccctcg
      961 tgagacactt caacgggttg ttgcaaccag cttccgggtc tgttttgatc ggggattggg
     1021 atgtgaaaaa acattcggtg gcgaaactcg cgtcgcgagt cgggtacgtg tttcaaaatc
     1081 ccgatgagca attgttttca cgcgatgttc tcaccgaggt cacgtttggg ccgaagaatt
     1141 tggggtatgc cagagagaag atcgatcaac tggtgaagcg ggcgctggca ttgactgaat
     1201 tgaacgacaa aaccgaaacc aatccctacg acctttcgcc gacgtggcgc aagatggtgg
     1261 cgctggcttc gatcgttgca atggatacgc ccatcgtcat tttcgatgaa cccacgacag
     1321 ggcaggatgc cgtcaacgtg gcgcggattg cgaatgtgat cgccgagttg cgcaaggaag
     1381 gcaagaccgt catcaccatt acgcacgata ttgatttttg cgccgagaat tttgagcgtg
     1441 tcatcgcgct ttcaaaggga caggtgctgc tggatggccc cgcccgcgaa atattggggc
     1501 aggaagaaat cctcgcgcag acgtacgttg acccgccgca gttgacgagg ttgggcaaaa
     1561 ggctggggtt gaaagagacc gtgagaaatc aggaagaatt tttaaattca ttgtaaggtg
     1621 taggggcgca gcgccgctgc gcccttttcc gttataggga cacggcaacg ctgtgtccct
     1681 acgttttata caccaccaat tcttcatatc ccgaatcgtt ttcaaataat tcccgcttgt
     1741 aaaccacatc ttcaaattca gcgatgacga tctcagccgt ggtgtaaatt ttgcacccgc
     1801 tgacgagatg cccgccgatg gtgacaccgt caccatccga gatcgccacg tggatgtgtg
     1861 aaccgtggat cgaaacagtg cctgtcatgg aaacgatctc gaaatggcct tcatactcgt
     1921 tgtaatggct gcgattcgcc agccgcaaag tggcatgtgt gaggctcccc acagacgaaa
     1981 gcacacaacc tgcctgtata ttattttgtt tgacgaactc ttcgatcgag tcaaataaat
     2041 cctgtgcggg tttgaggcgg aaggtatggg ctttcataaa ggctccttgg taaaatcgcg
     2101 gatatgaaat cttatcgtta tttcgacatc attctcggtg tgttcatcgc tgtgctgttg
     2161 atcagcaatg tcgcatcttc agccaagatc gtggattttg gtttcggcat cttcggtatt
     2221 ccgctggcgt tcgatgcggg gacaattctt ttcccgatcg ggtatgtctt cggcgatatt
     2281 ctaaccgagg tctatggtta tcaacggtcg cggcgcgtga tatggacagg gttcttttcg
     2341 ctggcgttgg cggcgttcgt gttttggatc gtgggacgtt tgccggggga agtcacctgg
     2401 cagggctatg caggtgatgc ggcgtatcag tccattcttg gcgggatgag cagcggcggc
     2461 attttgtttg ccagccttgc ggggttttgg ctgggtgaat tcagcaattc cttcatcctc
     2521 gcaaagatga aagtgtggac gaacggccgc tggctatgga tgcgtaccat cggaagcaca
     2581 ttggtgggcg agatgatgga cacagcgata ttcgtcgtgg tggcgactgc tttcggcgtt
     2641 tttccgtgga gccttttcct cacactgacg gtgacgaatt atcttttcaa agtgatggtt
     2701 gaagtggtga tgaccccgct gacctatatc tttgtccgcg ctttgaagcg ggcagaaaat
     2761 gaagattatt acgaccgtga gacgaatttc aatccgtttg caataataaa atagcgcggg
     2821 atattatccc gcgctacgtg tgattaccag aatgaaaaat gtgtcagtgc agtgaaccag
     2881 tcgatcatgg gctggtagat ctgcatcccg atcagtgcca cgatcacgcc taaaattgcg
     2941 cctgcgatga catcagaaag atagtgcacg cccatggcca cacgtgccaa agccaccagc
     3001 ggcgcccaca cccaaagaac agcagcaagc cagggcgggg caagaaacga ggcaaggacg
     3061 gcgatcaaaa acgaacgggc ggcatgtccc gaggggaagg agtgcgggtc ggtgttgcgg
     3121 tagattccgc cccattcgcc ttccggtcgc ttgcggcgga cgaggaactt gattccaagc
     3181 acaacacccg ccagcccaag gatgccgaag aattcaacga cttcccagtg tttccaaaac
     3241 gaattgctga aaaaccaaaa aagcagaagc gctgcgaacc aaaaccacga gtcgccggag
     3301 tgggcaaaaa aggcggctac attccgcaaa aaacccggct tttcagccac acgcatttgg
     3361 ccggagagtc gggcatcgag ttcaagaaga gaccgaaaac tcatttggtg ctgacctttg
     3421 cctttggttt tggtttgggc tttgcagcag cagaagcggt ggtctttttc gttttgctga
     3481 caggttcggc ggcttttttg gcggcggcga ctttcttctt ctctgccttg gcagcctttt
     3541 gtaattgact ggcgatgtct tcacgcatca gttcaagttg gtggggcttg tccacatcca
     3601 tgcagggttc ggccttgtcc catacgatgg cgcggccttt gatgccgatg cgctgggagg
     3661 cgcgttcgac cagcccttgc agggtgaatt ggcgagtggc tagttgaaag agagtatccc
     3721 aaccgatgac ggcggcctga cgcagggggc tcttgcggtt gccgatgagt tgttcccagg
     3781 tattaaggtg atcggtggcc atgctgacgt gaatcacgtt gatatcggcg ccgcacagtt
     3841 ccatttcctt cagtttggtg taggtgcgtt tcgatgtggg gtagcgttct tccatcactt
     3901 cacgcggaca gacgccatag tacaggtcat ctttggtgct catgcaggta tccaccagcc
     3961 agtccaccat atcgcctttc agggcgggga tgtctgagga aacgatcaac acgtattcat
     4021 tctttttatt taattcaagc gacttgttga ccccagcaac gatattggcg agcatgcgtc
     4081 cctgattgga gatgtagtgc aggggcttct tgcaggtcaa tccgcttttg ggcgagaggc
     4141 cgatgacgat gacattgtcc acttttttcg attcgcccaa cgcatccaaa acccactgga
     4201 tcatgggctt gcccgaaata tcgatcagcg cttttgaatt gccgttggaa tatacatata
     4261 aagggtcacc gggttgtgga attccaccgg cagttacgat tgcatccata attaattcaa
     4321 ctcctgcagt tggggttcaa aaccgagttt gtcgagcagt tcgttcagtt catcgaagga
     4381 aagcggacgg cctttgccgg agatcgccac cagcgcagct tccatcatgt tcgtgccgaa
     4441 ggaacgccca tccatgaccg gggtggtggt gatgaggtac ttgatgccgc acttgcggaa
     4501 gaattcaaca tcttccgggg tggtggtgtt cgtcactaca actttgccct gcatgttcaa
     4561 tggcatgtgg cgtttgatgt aaagacaatc acccgcgatg acggtggccc actgatacca
     4621 gttttcatac ttgggtttgt gttcgttctg tttatcgccg gtggggtaca gccattcgaa
     4681 gggaaagcgt gtcacgatcg ggatgagaat ggccgcgagg gtgcgcagtc ccttcatggt
     4741 gcggacgggg atgggcacat cgagtgcgaa catcaggtcg cagaaaaccg tttcatagtt
     4801 ggcttcggca aaggatttgg agaggcccca gcgatccaca ccgacggtta ccatgacctt
     4861 gcgtccgcgc tggttgatgt aatcgccgat ctttttgtcg agaaagccgg gggctttgtt
     4921 ttcaagcgtg tttttcaacc cgccgccatc caccacgggg gtctttttga tgtagcgaat
     4981 caatttttga acggaatgga atggatagaa cttgccttca acgatcgccc ccagatctgc
     5041 accaccgaca ccaaaggcgt ccactgtgcc gtccaattcc tgatatttca gggcggcggc
     5101 ttccatatcc ccatcggttc caatgcgttc gatgctgacc ttttcaccaa gcaacgtcac
     5161 ttcgacggcc ttgttgcgct ttgatgaacc gatgctgata ctcacggctc gtttcatata
     5221 tttagtctcc tcgtttgatg ggttaaagcg agtatacccc gaagtcaggt gcgtgagaat
     5281 gacattgaaa caggatcagg attcaggtcc gttgggaaac gtgaagaaga aagtcgagcc
     5341 gtgccccgct tcgctctgga cccagatcct gcctccatga acctcgatga ttctcttaac
     5401 cagggcaaga ccaatgccag tgccttctga agaggcatcc agtttgttga acaggccaaa
     5461 gattcggtca tggtgaattg ggtcaatgcc gatgccatta tcgcggatgt aaatgatcgg
     5521 ttgctcattt tccagcccgg cctgcccgat ctcgatgtga gggtcgggtt gtccttcggt
     5581 gaatttggcg gcattatcaa taaggttttg aaatacttca agcaggcgtt gatggtcgcc
     5641 ataaatcgtc ggcaggtcgc cctgggtttc cactttgaca tgggccttgt tcaatcgtcc
     5701 atgcagagct tccacagcct cggtgataag gtcttcaacc ttgatgaaat gatatgggct
     5761 ggtgagccga ccaatgcggg aaagttcaag caattcactg agcagcaatt gcattttgtc
     5821 ggtggcatca ccgatccgtt gaatatcaag tttcaggcgg gttgtgtttc cgctgagtgc
     5881 atcctgctcc agaaaaccga ggaagccctt gatggtaatg agaggtgatt tgaggtcgtg
     5941 cgaaacagtg taggtgaagc gttccagttc gctgttcttg tcttcgagct cgttaatgag
     6001 cttttcgcgt tccatggtca catatttttg ctggctgatg tcgcgcacca tgatgatggc
     6061 agattcaggg ctgatggcgg tgatgcgtgc ctcaaagaat tgcacttcct cgccgggcgg
     6121 cattccatat tcgaaggcat gcagatgccc tgtagccagg gtgcgctcaa gtgcgaacag
     6181 ggtttgctgg gcaatgatgg gtggaaatag ttcctgcacc gtcctgccca aaaattgtga
     6241 tggcgacatg agaggcacaa gctgggagga agccataaaa tcgagaaata cgccgtcctt
     6301 tgaaatttcg aagatcatat ccggtatgga ggcaaggatg gcgcgggtgc gcgcttcgct
     6361 gttttgcagg gattcgcgcg catttttttg ctcggtaata tcatggaaca tggaaaggat
     6421 gctgtctgtg ccttcgagtt tcaccagttc ataaaaggcc agtactgtgc tgtagagtcc
     6481 ttccctctcg ttcaggaata aatactctgg aacatagatg gagcgttcgg ctttcaagcg
     6541 ttcaataaaa tcaccgggtt cctcttcatc ctgccataga cccaattctg tggcctgata
     6601 cccaatggtt ttttgcaggt caagcccgga taacctccaa tacgcctcat tggcttcgat
     6661 gatggtgcct tcgctcaaag aagtgatgac aatggccacc gggctggcat ggaatacctt
     6721 gcggaagcgt tcttcgcttg agcgcagggc agcttcagcc atggtgcgtt cggcgatttc
     6781 tttttgggtc tgttcaaaga gtcgggcatt gtcaatggca agggaggcca gggctgcgaa
     6841 gcgctccatt aaattgacgt gatcgtgtgt aaaggtgcgt tcctgttcga caaatgaaat
     6901 ggccagcaca ccgatgacct tgttccccac tttcaggggg acacccatca ctgcattgaa
     6961 acccgcatcc acgaactctg gcaggttttt ctcccattca ccataatttt gaatgaccaa
     7021 agtcttgccg ctgccccaaa cctcccctgt cactcccaca tttttttgtg tcaggctgcc
     7081 atcgtaacgg gagagtacgc catgccccaa ttcctgtcgc agagcggatt cgtcgggcag
     7141 gacgaggtcg atcaacccat gttgggtatc cagcagggca caagcccgtg aaaggatgga
     7201 ttcgagcaag ggcctgagtt cagaacggtt taacaaaccc agagcagtct catttaacgc
     7261 ggtaaggtaa tcggcttgta cctgtaattt tcgctcggcg cgcaggcgtt cttttaattc
     7321 cagccgcgca tcatacaggg tacggttcaa actgttgatc aaaagataga tgagtgtgcc
     7381 tgcaaaaagg aaaatgaccg tgaaatcgcg ggcaaggttc aacggcgtat ccatgtgttg
     7441 aacacggagt ccccactctt cttcaactgc aaagaaccaa agtgctgaaa gactgataac
     7501 cccaaagaac aagccttcac gccatcccag gatcaaggct gaaagcagaa tgataagaat
     7561 ataggaaatg acagcggcat cacgaatgcc gtctgcggcc catgcctgaa aggtgatgat
     7621 gacccaaaat gaccccgcaa gaaagatgct tgcaccacga atgtagccgc ggcgaatgat
     7681 gacctggacg attcccaacg taaccacgat ggctgtcaac accagcaatg gcatggggtt
     7741 ttcttcctgc ccaatgatcg cccgtatgat gatgatgatt aaaagaatac tgattgcact
     7801 ccacacaaag ctgttgagaa tttgcgcggt gcgggttttt tcctcatttt caaaaatggg
     7861 ggatgagagc agtcggcgga accgatttgg catcctcgat attatatatc atgtatacat
     7921 aagtgcgctt tcaaaaaagt aagcaaacta tttagcttcc atgttgaacc gatggacgac
     7981 attcaagtaa aacgtcgaaa gataattaat atatgatgac cgcagacggt agaacgtggt
     8041 tatcgattcg gaattttagg aaagtgctgg cttaataaca gtgaagaact ttataacttg
     8101 cgaatccatc ttctctcggt tatccttgca aacgggagtg gttaggtccc ggtgggcttc
     8161 ctggtcttca acacctgtgg cgggtcgcgt tgcgggccgc ggtgggttcg actcccatcc
     8221 actcccgcct atgaattgga cgatggatga caggtggtct tgttgtccat cgtccgctgt
     8281 ccattgactg ttgattgaaa aggtatgcaa tgtcaattcc tgaaactgaa atccttacca
     8341 accttgtgtc gcgggtcttt cgcatcgaag atgtgaccag tgaagatccc cgcaagggat
     8401 ttttcctgcg ttaccgtggt gtattggcga atgaagattc tgcggcgctc tacgataccc
     8461 tggccgattc gctttcaccg tatggcatca ttccgctgtt tcgaatggag gagggacgtc
     8521 aagtcgttta tcttgcgccg aagccgccag agccaaagca ggatcgaatt tccaccaaca
     8581 tccttctctt cgttttgacc gtgttcagtg tgatgctggc aggggcacaa cccgaaggca
     8641 caatgccaga tgatttcatg ggtcaattgc tgatgctggc caaaagtatt ttcacgggtt
     8701 ggccttttgc attaagcctg cttggaattt tgttgacgca tgaactgggg cattatttca
     8761 tgagccgtta tcacaaaacc cccgccaccc tgccgtattt cattccgctt ccgctcagcc
     8821 ttttgggcac gatgggcgcg gccattatca tgcgcggaac ccctaaaaat aaacgcgtcc
     8881 tttttgatat tggtgtggct ggtccgatcg ccggattgat cgtggcattg ccggtgcttt
     8941 tttatggttt gtcactttcc acactgggca ccattgaccc caacccgaac gggttcatcg
     9001 aaggcaattc actcatgtat ttactggcga aatacatcac cttcggacaa ttgctgccat
     9061 ccccgctgga accgcaggga attttatact ggttgcaata tttcttcacg ggtcgccctg
     9121 ttccctttgg cgggttggat gtcttcattc acccggttgc atttgcaggc tgggcaggtt
     9181 tgttggtgac tggcctgaac ctcatccccg ctggtacgtt ggatggcggg catgtcatct
     9241 atggcctttt tggcgagaag gcacgcaagg gatttccttt tatcattggc attctggcga
     9301 tactcggcat tttctggagc ggatggtggc tctgggcttt gttgcttttt tggctcggca
     9361 gagttaatgc agaaccattg gatcaaatca ccacgctcga cccgccccga cgcctgctcg
     9421 gctatatcat gatcctcgtc ttccttttgg tattcatgcc tgttccattc atgacactgg
     9481 ctcaataaat cagcgatgaa aaagataatc ctctcattta ttatttttac gttcgtcctc
     9541 gccgcatgcg gaacgcagga ggatgtcact tccacggcgg agcctgctgt tacgcaggcg
     9601 gccacgaaaa cgccgcagcc tgaaccaacc gtcgaagcag cgagcaggct tggggtcggt
     9661 gaagaagcgt tgaatggatt ggagatcaca gtctggcatc cgtggtacgg agtcgaagcc
     9721 agtctgttcg agtcatttgt tgaagatttc aacgaaagta attcatgggg catcaaagtg
     9781 gttgcagaga gtcaggtcag ttttacgaac ctgtacgaaa acctgatggc ttccataccg
     9841 ggtaaggatc gtcccgatct ggcgatcgcc ctgcccgaac atgccctgga gtgggataac
     9901 aacggcatgt cggtggactt gatgccgtat gtgaatgacc cagcctatgg tttggattca
     9961 agcaatatcc ctgttgtatt ttggaatcaa gacctcgatg gcgaaaaacg agttgcgctg
    10021 ccagctcagc gcacggcgcg ctttttgctt tggaatgaga cctgggccga tgagatcggg
    10081 tttgcctctc cacccgatgc gcccgatgat tttcgccaac aagcctgccg tgcccagcaa
    10141 ttcatgttaa aggatgaatc gccgcagaac gatttcaaag gcggttggta tgccgataca
    10201 gattacatga ccgcttatgc ctggctgctg gcgtttgaag gcggtgtgtt ggaaggcagt
    10261 gattatcgct ttctcacccc gaacaacatc gaagccttca aatttttacg tgaactttcc
    10321 gaatcgacct gttcctggca gtcggcagat actgatgcgg tgaccgcatt tgcgaatcgc
    10381 gaggccttgt ttatcactgc cagccttgaa gacctgcccg atgccgcccg cgcatttgca
    10441 agcgcaggca acaccgatac gtggaaggtc ctctccttcc cgggtgaaaa cgaagatgcg
    10501 ctggtggtct acggttcttc ctacgttatg ctgaaatcaa cgaacgaaga acaactggcg
    10561 gcatggttgt tcgcccgttg gttgacagat gaaaaacagg atgcacgctg ggtggaggcg
    10621 actcatctct ttccgcttcg cactacgacc ctcgacctgc ttgccgatta caagaagact
    10681 cacccccaat gggaacaggc ggtcaaattg ctgccacagg gcgagattca accacaattg
    10741 gattcctggc gaaaagtgaa gatcatgctt ggcgatggct tcacccacat gtatcgggtc
    10801 aacgtcccca gcggacaggt ggccgccatc cttgcgcaaa tggaagccct ttcaagggag
    10861 ttgagcagat agatcattca gcgcagagca tgttgtgcca gatgagtgag aatctactta
    10921 actgcgaaac tcgccaagag tgcccttaaa tctttgtgag ctttgcgttc ttggcggtgt
    10981 aattattttt ttcgcttatc ttgatgtgta aatttgggcc gccgtgatga tatttttatt
    11041 gaagctggta aggcgctttt tttataaagg agacttgtat gcctgaatca gaatatcgcg
    11101 cccgacaagt ccgcgcgaaa acaaacgaag tgttcgagta tgaccgtgat gatgaaaagc
    11161 ccggcaaacc cctgcatatt ttgatccccg gtgggttgat tgctctggca accctggcat
    11221 tgatcgtttt tatcgcgtat caaacgtggt tcgagggaaa tcttgcggag aacatcggcc
    11281 tgattttggt cgtcatcctt tctccgttct acgtcggcgg agtgttcctt tttagttatg
    11341 gatacgagct ttacaacatt ccgcgcgccc tgcggttgac ggcgatcatc gtgtttgtga
    11401 ccctggcggc ggttgtgatc gtagccgtgc tgttctttct gctggcaaat atgaatggca
    11461 gttcgggttc caaatcttct tcttcaaatt cttccaaagg tggtaaatca cggtcatctt
    11521 catcctcgtc ttccagtaag ccatccaatt cgccaagacc ctccacgggc aaaatgggcg
    11581 gaattgtcgg cgcggctggc gcggctttga acagatcgtc caccaactct tcctcctccg
    11641 attcatcttc ctccagcgga tattcttccg gcggacatgt gtatattggc ggcggggaaa
    11701 cccgtgaagt gacaaaggaa gtggtgaagg aagtgcccaa agagccaatg cccgtcacct
    11761 gtccattttg cgcacggtct tacgtgcccg tggaaaataa ctatatctgc ccaagctgtg
    11821 gagcggcgac accaagggaa ttgatcaaag agtctcagga accgaagtaa agaatggaaa
    11881 acgaggtgtg ttgatgctgg gtgtgataat cacataacat cttcacggta taatcagaaa
    11941 aggcagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa agccattaag
    12061 aaaacaaaga ataatgacgg acgttctgaa tatgatttca gtaaggctca gcgtggcagg
    12121 ttctaccgcc cactggataa ggggtacaca gttcatgtgc gtaaaagtga cggaacggaa
    12181 gtcgtcaatc attacacatt aaaagaaggt acggttttac ttgcgccaga tgtactcgaa
    12241 tattttccag attctgagtc tgtaaatgag gctttgcgtt ccctgattca cttaatggag
    12301 aatgtccccg tgaaaaagta tagtgtttcg aaaatggcta cacatcaagt ggcggagaga
    12361 aaataaagat atagaccctt gttgaaaact tactcggcgt ggaagttgct tcccgccgag
    12421 gatttattta caggaggcaa ccatgcgcca gatgcttatc aatggaaagt tcacaaatgg
    12481 aaatgcaacg gaagagatcg aagttcaaaa ccctgccaca gaagaggtga tcgagtcggt
    12541 cccgcgcggc accccgcagg atgtggagga tgccgtacag gcggcgaaat ctgcatttga
    12601 ttcgtggcgc aagctggggt caaatgaacg tgccagattg ttgcacgaag tggcagacag
    12661 ggttcatgaa catcgtgaga agatcgttca catgctcacc ctggaagaag gcaaacccgt
    12721 ttctgaaaac gaagaagaag tggaatgggt catcaacacc ttccactact acgctgagtt
    12781 gggccgtcat catcgtggca gtgtactggc ttcgggtgag gcttctcaat tcaatttcat
    12841 catcaaggag ccgtatggtg tggtcggctg tattgtgccg tggaattatc cgctgctcct
    12901 gctggcatgg aaggtcgcac ccgcacttgc ggctggcaat accgtcgtca ttaagccgtc
    12961 agaaatgacc ccgttgacgg cactatatct ggccaagcat tgttttaatg ggcttcccgc
    13021 gggtgtggtc aatgtggtca caggctatgg gcttgaaaca ggcgaaccgc ttgtcaaaca
    13081 ccccgacgta cctgtcatcg cttttacggg cagtctcgca acaggtcaac gcattgcttc
    13141 gatcgccgca ccaatgatga aaaagctgca cctcgaatta ggcggcaagg atgccaccgt
    13201 cattgcagag gacgccgacc ccgagatcgc cgccaaagct gtggcatacg cggctctcat
    13261 caacgccggg caggtttgca catcaacaga aagagtctac ctgccgaaga ctcatgcggc
    13321 gaaattcacc gaagccatcg tggagcacgt caaatcgctc aaactgggcc ccggcctcga
    13381 ccccaaaaca gacatgggac ccatgatcgg cgaaaccttc cgcgccaaat ttgaaaagca
    13441 acttgtggat gcgcgcgcca gcggggcaaa gatactcacc ggcggtgcgc ggccacaata
    13501 tttggaaaag ggttttttcc acgaaccgac cgtgttgacg ggtgtgaatc acagcatggt
    13561 catcatgcgc gaggaaacct tcggccccgc cgtgccgatc atggaataca cctcttttga
    13621 cgaagccatt caattgacca atgactgtca atacggtctg ggagcggttt tgatctccaa
    13681 cgacccgaaa aagatcaaac gctttttcga cgatgtcaaa gcaggcacga tctggatcaa
    13741 cgaccccctg accgataact acgccggtcc attcggcggc atgaagtact ccggtggcgc
    13801 acgcgaactt ggcgaggaag gcctcgaaga attccgcgag accaagcatg tacattggga
    13861 tttcaatatg gaagaaaaag gatattggtt tccatacgga cgctagcatt ttcatcaaat
    13921 accagccagt agtcggtcca gtttataatc tgagccatga gaaaacaatc atggctcttt
    13981 ccttttgtat tgctcctggc agtgattttg tatttccaga tttcgcgtgt gcagttaccc
    14041 ggtgatggat tttccacctt cggctcggat accgtccgtg cggaggtgat acagattatc
    14101 gaagaaggcg aagtagacct tggtggtcat acccagaaat atcaaatcgc gcgtgtgaat
    14161 attttggagg gggaatatga aggcattcca atggaaattg actatggcaa gcgtcaactt
    14221 cgttcagatg attatgacct tgtcgtaggc ggcagggtga tggtgtccat tagcaagaca
    14281 ccggacaatg tagttaatgc ctattttgtc gatttcgtcc gcacttctcc gttgttgtgg
    14341 ttgacggtca tttttgcggc ggcaatcatt ctcatcagcc gctggaaggg ggtgcgcgcc
    14401 ttgttgagta tggcatttag tttgtatgtc atcattgacc agatcatccc acatattctc
    14461 aatggcgatg acccgctttg ggtgagtatt atcggttcga tcgtgctgct gagcgttaca
    14521 ctgtacctca cctatggctg gaatttgaag acacacgcct cagtgctcag tatggtgctg
    14581 gtattgctgc tcacgggggc gctttccgcg gtgttcgtca ttttcaccaa gttgaatggc
    14641 acaggggacg aaaacgtcat gttcctgatg caaatgatgg aaaaaccgct caacctgcgc
    14701 gggttattgc tcggcggtat gatcattggc gcacttggcg tgctggatga tcttgtgacg
    14761 acgcaatcgg cggcggtgtt cgagctgcac catgccaacc cgagttttaa gttccgagac
    14821 ttgtacaact cggccatgcg catcggtcag gaccatgtgg cggcgacggt caatacattg
    14881 gtgctggcgt atgcgggagc gtctttgccc atgctgttga tgttctcttt agggcatggg
    14941 aattacggct acattatcaa tttttcgttt attgctgaag aagtggttcg gactttggtc
    15001 ggctcgttgg ggctgatcgc ggctgtgccc ttaaccacag ccattgccat tctttttgct
    15061 caaagggccg agttgcttgg acggtgggag caggttctcg gtccagaagg aagcggggat
    15121 gggcatggac acgcacacta actgcgcata ggcgcttggg ctgacgaggg ggaatccagt
    15181 aatttgcatc ccgcttggcg caactttctc tcaccatccg cgtaaaagaa gtagaaagtc
    15241 tacgaggagc acgcgtgaac gaagaacaga cctgggtgat ccaggcacaa caaggcagtg
    15301 acgaagcatt tactcatctc gtcgaaacct atcagacccc tgttttcaac ctgtgttatc
    15361 gcatgttagg cgaaccagat ctggctgaag acgccgcgca ggagacgttt ttgcgcgcct
    15421 tccagcacct gcatcgctat gaccagaaac gttcgttcgc tacctggctg ctttccattg
    15481 ccgcacacta ctgtattgac aggttgcgcc gccgtaaatt cactattttt tcgatggata
    15541 cagaggacga ggacggtaac acctttgagt tacctgacct cgattcaccc aacccggaag
    15601 ctgaatccat caaagggcag actcgtgacc ggatccaggc catgctcaaa gacctcgacc
    15661 ctactgatcg cgctgccatc gtcatgcgtt actggtacga ctattctgaa gttgagattg
    15721 cagactcttt acgattgacg gtgagcgcgg tgaagagccg tttacaccgc gcacgcaagg
    15781 aacttgctgg tctttggcag gaagaagaaa cccgtgctga gaccgaaagg agaccctatg
    15841 aatcaccagc cttttgaaga atggctactg aacgataaga acttgaattc cgccgaaaag
    15901 cgtgaactcg atctgcattt gcggacttgt gcccattgca ccgcgctttc tgccactggt
    15961 ctggcattgc gctctgccaa tgtggttgca cctgcttcgg ggtttgccct gcgcttccaa
    16021 cagcgtttgg ctgcacaacg aattgccgaa cgccgccgca agttgtgggg ggcgatggtg
    16081 ctggttttga gcggatttgc cctgctgaca tggtttgtgg cgccatttgt gtatgccttt
    16141 ctaaattccc cggcagaatg gattacggcc gcagtgagct attttctatt ctttgcaagc
    16201 tcagttcaag cgttgaccga agccgccgcc gtgttattcc gcattctgcc tgactttgtc
    16261 ccgccttata tgtggatggt gttcgtttct gcgctggccg gcctgggcct tttatggaca
    16321 atttcaatct ggcaatttag ccgcaccccg caaggagttt cagcatgaaa agtaaattaa
    16381 cgaccctttt tctattacta gctttattgt ttttacccac tggagctgtc cacgcgcaaa
    16441 gcccggacgg tggcgatgtt tttctattgg ggcagaacta cacccttaag agcgatgaaa
    16501 cactggatgg cagtctggtt gtgatcggcg gtaatgtgga tatcgaaaaa gatgccacag
    16561 tgaacggcga tagcgtgctg atcggcggca atttacaagt caatggagat gccaacggca
    16621 gtgtggttct gatcggtggg aaccttgtga tcagcggaaa aatcaaaggt gacattgttt
    16681 tgatcggtgg tcaggctttg ttaaccgaaa ccgctgttgt agatggcgat attgtgacaa
    16741 tgggcggtca ggtgcaaaaa gatcccggcg ccgaagtaac cggccagatc gtgaataacg
    16801 ctccgccaat tgatgttcct gatattcctg acgtgccaaa tgtgcccgac gcacccgatg
    16861 cccccgatgc gcctgatatt cctgatgtcc ccggggtgcc tcagatcaac gttgatttca
    16921 acccgttatg ggaagtgaca ggtgttttca cccgtgccct ggccattgcc gccgttggca
    16981 tgctgctgac cctcttcctc cagccgcaat tagatcgtgt ggcagatgcc attacacgtc
    17041 agccgatgat ggcgggtagt tttggcctgc tgaccgtggt cgttgccccg gtggcgatcc
    17101 tgatcatgat cgtgaccatc ctgctcatcc ctgtggcagc catcgtggcc tttattctgc
    17161 ttccaatggc ctggttgttt ggtatggtgg ccatcgggca ggagatcggt gaccgcttca
    17221 ccaaggccat caatcaaacc tgggctcctg tactttcgac aggttttgga accttcctgt
    17281 tcctgttggt cggcggcttt attgggctga tcccctgtat cgggtggctg atctccctta
    17341 tgttctcact cgtagccctc ggcggtgtga tgatgacgtg gtttggcacc cgtgctgccc
    17401 ccggctcggc tcctgcggtg gtaacagaaa cgatcccgcc tgcttcgtaa atagccgttt
    17461 tcaaggttgt ctggcgagag ttgctaagtc tgtgggctaa ccacaaagac gccaggccac
    17521 taagccacca agaatccttt gtgtcttcgt ggcaatcaaa accctttagc ttcctgtttg
    17581 gcaagccaaa gccgaaaacc aaacctgcat gatataatgc gcccgccccg gggaagttcc
    17641 cccggggcgg ttttcatgat ggaggaaaac atgtttacaa agcaagaaat tcccaatacc
    17701 ccaattcaca acaccccgcc gcctctgatc gaggtttcag gcacacaccg cgagatggga
    17761 cgccagatcg gtgaggccgc ccgcgcacag gtgcagcata gcgtggccaa tgcccgcgtt
    17821 ttgatcgacg ccgcctacga cagccttgaa ctcacctggc agggcgcgca gatccaatca
    17881 cgcaaatacc tgccttttgc cgaggagcgc tacccgcaat atgtggatga aatgcgcggc
    17941 attgccgagg gcgcaaatgt tgccttcgat gacattgtca cgctcaatgc aatggaagcc
    18001 gtcaccgtgg atgcgcttca cctcacccgc tgtaccagca tggcagtcaa cgaagagcgc
    18061 acagccgatg ggcatgtgct ggccgcccac aacgaagatt gggttcccga agatgaagat
    18121 ggcgttctgg tcatttcagc gaagcccgac aaggaacccc cgtttcttgc catgacctat
    18181 ggcgggctgc tgcccaatgt gggattcaac gcctttggca ttgcccaact cattgactct
    18241 gtttatccga gcgactcgcg cattggcatt ccgcgcctcg tggtggcgcg cgctgtcctt
    18301 tcttcgcatc gtatttcggg cgccattggg cgtaccctcg ttcaacatcg cgcggctgga
    18361 tataaccacc tgctgattca cgaaagcgga gaaatgtatt ccatcgaggt ttcggcgcgt
    18421 aaatttgaaa ttctgtatgc gcacgatggg tacatggtcc ataccaatca ttatctcgac
    18481 ccgcaaatga agcagatcga gaaagacccc gaagaattgc tctcttcccg tgtgcgttac
    18541 ttccgtgcct cgcgcctgct gcgccagaac ggtcagcaca ccatcaagtc cttgcaggcc
    18601 attcaaaagg accacaccaa catccccaac tcgatctgca accacaacat cgagggactt
    18661 gatccgctcg accgcgaaaa gaccatctcc gcccttgtca ttgacctgac ttcacgtgaa
    18721 atgcacatca cctggggaaa cccctgccag aatgcgtatc acacttatca tttgaatgcg
    18781 taaggtgtaa gaaagtgaaa agaaaaagag tctgtgcgcc cggcacagac tctgaatttt
    18841 taaatggcaa attcatgcct tcgggattat cataagcgct tctattggag gtcccatgcg
    18901 agatttaaca aatgaaatcc ttgaattgat tcgccgcaca tcttcaagcc tgccgccaga
    18961 tgtggagaaa cggctgcgcg aatccattga aaaagaggag cctggttccg ctgcccgcgg
    19021 ggcgttggaa accatcctca aaaatattga cctttcccgg gcaaattcca cgccgatttg
    19081 ccaggatacc ggcacgccta ttttctatgt gcattacccg gtgggctggt ctacgatcaa
    19141 gctcaaagag cagatccgct ctgcgatggc cgaagccacc aaacggtcct atatgagacc
    19201 gaatgccgtt gatgccatct acgacaaaaa caccggaaac aatctcggcg gcgatgactt
    19261 cccctatgtc catttcgaag agacagaagg cgatgacctc accatcgaat tgatgctcaa
    19321 aggtggcgga tgcgaaaatg tcggccgtca atattccctg cctgataatt ccgtcggcgc
    19381 ggggcgtgac ctggccggtg tgcgcaaagt ggttttggac gcagtgcaaa aagcacaggg
    19441 acagggatgt gcgcccggta ttttaggtgt tgccatcggc ggtgaccgtg ggtcgtcgta
    19501 tatcaaatcg aaggaagtgt tgttcagcga aatgggcaca cgcaacaaag acccgaaact
    19561 ggctgaattg gaagaacgcc tgacgagcga agccaaccaa atgggcattg gcccgatggg
    19621 gttcggcggc aagaccaccg tgttggatac gaaaatcaca gggttgagtc gtttgcctgc
    19681 ttcgtatttt gtatctgttt cgtacatgtg ctgggcctat cgccgccgca agatgacggt
    19741 gaagggtgaa gagatcactt acgattaacg attttcgatt tacgatggtg cggttcgtaa
    19801 aaaggagaat gaaatgaaac aaataaaaac accgatcacc gatgaggtca ttcgtgaatt
    19861 aaaggttggt gatgcggtca gcctgtcggg tatgatggtg accgggcgcg atgccgccca
    19921 caaatggatg atcgagactt tcatcaaaaa gactcgtgct ccgcagggcg atgacctgca
    19981 agtgtatgaa gaattgaaaa aactgctggc tggctcggtc atttaccact gtggccccgt
    20041 ggtgaccgga ttggatacga aggaatacaa gatcatcgcc gctggcccca ccacatccat
    20101 ccgcgaagaa ccgtatcagg cggatgtgat gaagcatttc aatgtcaaag gggtgatcgg
    20161 caaaggcggc atgggcgcca agaccttgaa aggctgtcag gatacccccg gggtgtattt
    20221 tcatgccatc ggtggtgcag cctcttttct ggcgcagacc gtgaaaaagg tgcatggtgt
    20281 tttcaaactg gatttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagtaa
    20401 atccggcgaa tataaaaaga tcgagatgat cgcaatcaac atacacagca cacaaacaaa
    20461 aacaaagccg ccgatgatga ccccggtcca tgccatgggg tggcctgttt cgttccgctg
    20521 acggatctgc cgtagtgaaa tcgtcccata agtcagtgca agaatgccaa gcagaaaact
    20581 ggctgggaaa caaaggatcc ccgtgaatgg aacgggaagc cagcccgtgc aaaatgtcaa
    20641 cagggttaac actgcaaaga caaggctgag aatggatttg gtattgagag gggaggaggg
    20701 ctgtatttcg tttgagtcca tggaaaaata aaaccgccct gtaagggcgg ttccttttgt
    20761 gctgtcgggg cgagaggatt tgaacctccg acctcttgca ccccatgcaa gcgcgctagc
    20821 cgggctgcgc cacgccccga ttgagcgaag cggattatag ctgtatttca tcattctggc
    20881 aaatgcaatt tttcacggct ccgggtggca acctgcaacc ttccaccttc accctttatg
    20941 ctaaaataag cgcattggag ttgccttatg tcagatatga tagaagttgt aattgatagc
    21001 atacgtgtac acttgatgac accacagcgt gttgtcgttc tcaagcagac gaatgccgaa
    21061 cgctacctta ccatttgggt gggcccttac gaagcagaag cgatcaccgt ggcattgcag
    21121 gaagttgaaa tgtcccgccc gcttacccac gacctgctta aaaatatttt taccactttc
    21181 aacgcgcgca tcctgcgaat cgaaattacc gagcttcagg gcgaggtctt ctttggaaac
    21241 atcatcgccg aagtggatgg acgggaattg cgcatcgatt cgcgcccgtc tgatgcgatc
    21301 gccattgcgg ttcgtgcaca cgtgccgatc cttgtacatg cccatgtcat ggaatccgcg
    21361 ggcatggccc ccgatcagga tatgcctgat acatcgccaa caactccgct ggcacgccgc
    21421 gaagcccctg caccgctctc agatgatgcc aacaaacgtc tgtctgtttt caaggatttc
    21481 tttgacaaac tcgacattga caaccctgac cgcgacaaac ccgattcaac cccacctctg
    21541 gattgatcca catcaatgac agattccctc cccgaaacat tccccccata cggggatgat
    21601 tcatcatccg ccgcccccgc cagttcgacg tcttcgtcca tcccgcacag ccgcgaagcc
    21661 gaagaagccg ttgtaggggc ggtcttgatt aaccccgaaa tctattacga cgtagcccag
    21721 ttcctgagcc cggatgattt ctacatccac cgcaacaagt ttgtgtggga agccttcaca
    21781 cgtttgcacg aacaacgcat ccctgtggat ttgctcaccc tctccgaaga acttgaccgt
    21841 gtagggcagc ttgcagaaat tggcggctcc gcctacctga cgtcgctcat taatcaggtc
    21901 ccatcttcgc taaacgcgga atcttatggc cgcatcgttg aaggccactc catccgccgc
    21961 aagatgatca atgccgccaa caagatcgca tcggtggcat acaacgaagc ctccaccgtg
    22021 gaagatgtga tgaacgaagc ggagaaggcc gtcttcaacg tcagcgaacg ccgcctcaaa
    22081 cacgacctcc aaccgatttc cacggttttg agcgattact acgaccgaat tgacgacctc
    22141 gccaaacgcc cggatgagat ccacggggta cccaccgggt ttattgacct cgacaggctg
    22201 ctgacaggtt tacagccatc cgatttgttg attattgcgg gccgcccggg ccagggtaaa
    22261 acggggttcc tgctttcgat cgcaaagaat gcggcgttga cccacaaaaa gcatgtcgcc
    22321 atcttttcgc tggaaatgtc gaatgagcag gtggtgcagc gcttgatcgc ccaggaaacg
    22381 ggaatcgatt cacaacgcct gcgaaacggt aaactgcatg agcatgaatg gccgcttttc
    22441 acccatgcca ttgaagtgtt ttccgatacg catatctacc tcgacgatac tcccgccatc
    22501 accccactgc aattgcgcac caaatgccgc cgtttgcaca tggagtatgg cattgacctt
    22561 gtcatcattg actacctcca actcatgggc ggcgactcgc gcaacgataa ccgcgtgcag
    22621 gaagtttcac acatctcgcg cagtttgaag gtgctggctc gtgaattgaa tgtccccgtg
    22681 ttgactgcgg cacagttgag ccgtgccgtg gaacaacgca ctgacaagaa gcccgtgctt
    22741 tcagacctgc gcgaatcagg ctcacttgaa caggatgccg atatcgtcat gttcatttat
    22801 cgtcctgacc aatacgaaaa agacagcgag aaacaaaacg tggctgagat cattgtggcc
    22861 aagcaccgca atggacctgt tggcagcgtg gaaatgatct tccgcggcgc acaggcgaag
    22921 ttcgaaaatg cggccacgcg tgtattcaga ccgaacgagt gacaagggaa aggatgatgg
    22981 gtgaattttg aaggatgaag atcgcgctca tcctttttat aaacccataa agcaatggct
    23041 gctttttctg gttttacctc ttctgaaaca tttacccaga ttcctgattc gttcatccgc
    23101 ctgcttggtg agatcgaaga tgtggctgaa ttgaaggtgg ctctccatgc catctggcgc
    23161 atcgaacata tggagggaaa ttttcgggct ttgcgtgaat cggattttgc ggttgaaatg
    23221 cttgggttgg aggggaggga gatcaagcgc gggctgaaga aatgtctgga gcgcggcacc
    23281 ctgctcaagg cggagaatga atcggaggtt ctgtatttcc tcaattcccc gcgcgggcgt
    23341 ctcgcagttg attcttttac aaaagggaaa ctggctggca ttgcttcggc aacacgtgaa
    23401 agatcaaacc tgttcaagtt gtacgaggaa aacatcggcc cgttaacacc gttggttgct
    23461 gatgttctga aagatgcgga agaaatttat caggccgagt ggatcgctga agcgatcggt
    23521 ctggcggttg aaaataataa aagaaactgg aaatatattg aagcgatttt gaagcgctgg
    23581 aaggaagaag gccgtgccga aaagcaggat agacgaaacg ataaaaaaga tggccagcag
    23641 agcctcagcc gaaagatcga gcagctccgc aagcgcgccg ataaaaaatg atctgcctgg
    23701 cgacccgaac tgcccgcact gccacggtgt gggatatttg cgttcagatg ttcctgtagg
    23761 gcatgcggaa ttcggcaagc tcgaaatttg cacctgccgt cgttcaaagg tggccgataa
    23821 tatccgcgac cgtttgttcg cgatgagtca tcttgatgag ctaaaagacc tgacctttga
    23881 atctttcaaa ccgcgcggcc gcaaagggtt gggagagatg caggctcgtt cgattgaaat
    23941 ggcattcaac cacgcccagc attacgccaa aagtttaaat ggctggctgc ttttacaggg
    24001 agggtacggc agcggaaaga cccaccttgc cgccgccgtt gcaaattttg ttgttggcat
    24061 gggcgttccc actttgttcc tcacagtgcc tgacctgttg gatgccctgc gttttgcata
    24121 cggctccgaa gatacaactt ttgaagaacg ctttgaacag atccgctctg cgaaattgct
    24181 ggtgctcgat gattttggaa cgcaaaacgc cacaggctgg gcacaggaaa aattatttca
    24241 gatcatcaac tatcgctaca tcaataaact ggccacagtg gtgacgacca atctctcact
    24301 ggatgagatc gaaccccgca tccgctcgcg tctttcggac ccggaactgg tcagcccagt
    24361 gaaaatgtcg gccccggatt atcgccgccc aatggaagat accagtcacc ccgaactttc
    24421 ctctttggat ctgctttcaa gccgaacgtt cggttccttt gaagaccgtg ccgcagagaa
    24481 actcacgccg gatgagcata aatccctgca aaaggcgttg aagatcgccc attccttttc
    24541 tgaaaagcca aaaggctggc tggtttttga aggcacctac ggatgcggta agacgcacct
    24601 tgctgctgcc attgccaatt atcgtgctgg ccttggtgac ccgccgttgt tcgtgatggt
    24661 tcctgatttg ctcgatcatc tccgtgccgc ttttagtccc aactcaggga caagctacga
    24721 ccgccgcttc gatgaagtgc gcaccgccgg tctgcttgtt ttggatgacc ttggtgcgca
    24781 atctgccaca ccttgggcgc gtgagaaatt gcatcagctt ttgaactacc gctacaatgc
    24841 cgaattgccg actgtcatca cagtggcaaa ggaaaatctc gatcaaggtc agatcgatga
    24901 acgcatcatc actcgcatgc tggatgaacg cctgtgcgaa tatatcgaga tcaaagcgcc
    24961 tgcctatcaa gggaagggga aacgcaagat cggcaggaag tagccttttt aactgatatc
    25021 aaggaaacgc tgatgtacgc tggccaaagc ggaccatcag cgttttttgt ataatgggag
    25081 gcggcacggg ccgaatttgg agccttttcc tcaaccaaat tttcaacatg gttgaaaatt
    25141 tggttcaagt gcctatctag attctccccc tggtctcgtt tataaacagg gcaaataaga
    25201 ccaaaaaaca cacaaaggaa aaagaatcga atgctccctt cctatttgct ctccctgcgc
    25261 gaaggccttg aagccgccct cattatcgga attgtacttg gcgccttgca gaagatccgc
    25321 cgtacagatc tggctcccat gctttggtat ggcacatttg ccgccatcgg ggttagcatc
    25381 cttaccgcca ttttgctgac catttttggt ttgagcctcg aaggcagcgc cgagcaaatt
    25441 tatgaaggta tcaccatgtt ccttgcggca ggcctgctta cctggatgat cttttggatg
    25501 agccagcagg ctcgtaccct caaagccgaa ctggaagccg atgtgaacaa agccgccgcc
    25561 tcacgcaacg gccgcgccct gttttggatg gcttttgtcg ctgttgtgcg cgaaggcgtg
    25621 gaactggctt tcttcatcac tgccgccttt tttgcgggca ataccgagaa cgtcggcgcg
    25681 aatacgatcc agacgctggc tggtgtggtc cttggtttga gcacctccat tctgcttggc
    25741 tggtcactgt ttgcaaccgc tgtccgcctt gacctgcgcc gcttttttca ggtcactggg
    25801 ttcatcctca tcctttttgc gggtgggttg gtggcgcatg gaattcatga attcaacgaa
    25861 gtcggttgga tccccaatat catcgaacat gtttgggatg tgaatgcgat cgttgatgag
    25921 aaatctgtgc tgggtgaatt gctcaagact ctttttgggt acaacggcaa tccctctctc
    25981 accgaaatga tcggttactt tgtgtacctt gctgttgtgg cggtcttctt ccaacgcagt
    26041 aataccaaaa aggatgtgat ggtcgcccag ccttcggcat agagtggttg ggaaggtaac
    26101 tgattttcaa gagcggggtt cgcgcacagc gcgaaccccg ctctatgtta ataaagctct
    26161 tgacagcgac cgcaaaaata aatatactaa gtgtataatg aacactaagt atgaagctta
    26221 tgaacaaccc ggtatcaccg ttgaccttgc cattttcacg gtcaacaaca acaaactcaa
    26281 ggtaatgctg gtcaggcgtg ccgaagatcc ctataaagat ttttggtctt tgcccggcgg
    26341 attcatcaaa caaggcgaat cgctcgagga agccgcaatc agggtcttga atgaaaagac
    26401 cggtgtgaag aatgtgtaca tggagcagct ttacaccttt ggcgaccccg cgcgcgaccc
    26461 gcgctcaaga gtgatcacag tgacatactt tgccctcatt ccctgggctc agcttgcaca
    26521 acctgcatca gaaaaagttg ccgaccttgc ctggtcacat atcgatcgcg tcccaaaact
    26581 cgccttcgac cataaaatga ttttggatta tgccgtcaaa cggctgcgcg caaaagcaag
    26641 ttacagcaac atcgtctatg ggttgatgcc cgtgcaattc aggctttcag agctccaaac
    26701 catgtacgag atcatcatca acgacaaact cgataaacgg aatttcagaa aaagaatgct
    26761 ctctacaggc ctgctgcaag agaccggcga aaaagacctc accggcgcgc acagacccgc
    26821 catgttgtat caattcaaaa aaatggaaat agcttttctc gattaggtga accatgaaaa
    26881 aacaaaccct gccccttcct gaattttttg aatcggcccg tgtgggtgaa atctggaaag
    26941 tggattatgc cagccgtgcc gaacaagccc gtcaatacgc gcgtcaacac gacctgaagc
    27001 ctgcctctgc tgcaagtgaa aagatctccc ttttagtgat cgatgcacaa aacaccttct
    27061 gcatgcccaa ttttgaatta ttcgttggcg gccgcagcgg acatggtgcc gtggacgata
    27121 acgtccgctt gtgtgaattt atctatcgca accttggccg ccttactcac atcatcgcca
    27181 cgatggatac ccacatgtca cagcagatct ttcatcccat cttttttgtg gatcccgaag
    27241 gcggtcaccc taccccatac accgacattc atcttgcaga tttgatatcg ggcaggtgga
    27301 aattcaatgc agcacttgct ccacgcctta atatcgcccc cgagtacggc caacaaatga
    27361 tgatccatta cgctgaatcg ttggagaaga aaggcaaata tgcgcttacc atctggcctt
    27421 atcacgccat gctcggcggc atcggccatg ccttggtccc cgcattggaa gaggccatct
    27481 tcttccattc gatcgcaagg ttcgcccagc ctgatttcga gatcaaaggc aacaaaccgt
    27541 ttacagagca ttattccgtt attggccccg aagtgctgac tggccccatg ggcgaaaccc
    27601 tcggctctca cgatacaacg ttcatccagc atttgcagga agtggatcgt ttgtatatag
    27661 cggggcaggc caagagtcat tgcgtagcgt ggacggtttc agacctgctg gctgatattc
    27721 aagacgccga cccaagcctg gctgaaaagg tgtatctgct cgaagattgt tcctcccctg
    27781 tggtcgtgcc tggggtggtg gaccataccg aagcggcgaa tgaagcgtac atccgctttg
    27841 cgcaggctgg gatgaaggtt gtcaaatcca ccgatgagat cagttcaatg taaacatacg
    27901 ggtgtataaa cagtaaaaat ggagtattat gccaaacctg acccgcctct caaagttcgt
    27961 gtctgtcatt ttgcgtcacc gtgcgaatga ttttgacctt catctggatg cgcagggatt
    28021 tactgatttt gccgaattga aacgcattgt gattgaaaaa tccaccgatg tttatagcga
    28081 agaagactgg caaaaggttt tgaatggcga gctcgatgga aaaaagcgtt tcgaagtgaa
    28141 agacgggcgc atccgtgcga tgtatggcca tagcaaggtt gacccggtca gctacccgaa
    28201 ggttgagcca cccgaaattt tgtatcacgg tacaaccccg caggctgaaa agaatattcg
    28261 cagggaaggc ctgcgttcca tgaaacgtca gtatgttcac ctgtcttcaa atctggagcg
    28321 cgccaactcg gttggcagcc gccgcaccga gcatgtaacg attttggtgg tgcgcgccag
    28381 agaggccgcc gttgcgggag ttgagtttta tgcgcctgag cccgagcatt ttttagcaag
    28441 atccatccca ccgaacttta ttgatttccc acagaattga gaagccccat gtccattttt
    28501 gacggtaaga gactgacgaa cgaaacattc aagctcgata ttgatcgcat gcgccgcggt
    28561 tggtattcgg ataaatattt cgagaacatc aaccgcatgt tgaccgcgct ctcgaaggag
    28621 aattacaact attccgggag ttttcccatt ttgccgaatg gcatatctcc cgagcagatc
    28681 gccgtgggcg atatcgaagt ggagatgcaa tggttcacgc ggcggatggg caagaccacc
    28741 gtggtgggtg tggataagtc tcttgaaatg ctcaggcatt gtacgggtta tttcgatggc
    28801 gatacttttg tggatacatc tgatcggttg gaggtatggg cagtgcagga tggcagcatt
    28861 gtcaaatttg aaggcgaccc cacaaagatt caacctgtca tcaaggtgcg cggacgatac
    28921 cgtgattttg ccctgctcga aacacccacg ctgggcattc ttacacgttc cagccgtgtg
    28981 gcaacgaatg tgtatgaaac cctggtggct tcacgcggca agcctgtgct gttcttccct
    29041 gcgaggttcg atgttcatga agtgcaggct gcggatggat atgcctataa catcgccttg
    29101 ctgcgattta acaaggatca tgcccaggag ttgggcgcgt tcgtttcgac cgatgcgcag
    29161 ggtgactggt gggggggcgc tggcggcggg acagtggccc atgccgcgat tgcatccttc
    29221 cttggtgata ctgccgaggc gatgatgcag tttgcacaca tcctccctgc cagaattccg
    29281 cgcattgcac tggtggattt caacaatgac tctgtccgcg attcgctgcg ggtgttggat
    29341 gcgatgttcg cgaaataccg cgagttgatg gatgcaggca acagggagga agcggaaaag
    29401 tacaagcttt acggtgtgcg ccttgatacc agcggaagtt tacgtgatgt atcggtgcag
    29461 cctttgggcg accccgcttt ggatcttggg gttaacccgc ggctggtgtt taatgtccgc
    29521 caggggttgg ataatgcctg ggaaagctgg accctgccca cgtcgtggaa ggatgcggcg
    29581 cgggaatttt gtcataatgt gaaaatcgtt gtttcggggg ggttcaatcc agaaaagatc
    29641 cgtaaatttg aaaggcttga agtgcctgtg gatatttatg ctgttggttc gtatcttttc
    29701 aataacagca atagcacatc cactgattac acggcagatg tggtgcgcgt gaagattcat
    29761 ggagagtgga tcgaaatggc gaagatggga cgcaaggtcg gcgaaaacga aaaccttgaa
    29821 cgggtttggt gattgagtgg ttgatcaaat taaaaaaggc tcatgaaaaa cgttgccaaa
    29881 gcgtgtgcct gacgtagggg ttgcgtccag tatttttttg aggtgagtga taaagttgaa
    29941 ttctcttctc ctctggaaaa tgaaatggcc ggtgaacatc accggccatt tcatttttgt
    30001 aacgatagac tgttttttga atgagcgtta gttggctggg atcaattgct cggcgatctt
    30061 gagtaattcg tccataggta aattggtgag tagctcaatt tttgtgcnnn nnnnnnnnnn
    30121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30181 nnnnnnnnnn nnnnnnnnnn nnnnnnngtg acagcttctc catctggcag tgcatcgttg
    30241 tcggcggcgg gttgttgagt gatgattaag aacgtatcat ccactttgta gaggagctct
    30301 gaaacatcgc caaagcttgc agagtttata tctttgaaga tctcaggcag gtatccaggc
    30361 tgcattaact gatatttcat tggctcgttg ctgacgaagc caaaactcaa gttgctgttg
    30421 ttggggcttt gaaatttgac aaaaaaccat tcctcgactt gtgcccgcac cggggggaca
    30481 aagacaacgg tggcggtcag tgcaagaagc atggccaatg tcaaaaatgc atatttgtat
    30541 ttcatggttg tagtttttcc tgttctctta acctgccagg gagcggtttt cattcttgaa
    30601 taaaaaccct cgccaggcat gggctgaatt gtggataata ttttttcgat ctgatgttca
    30661 tcgggcatgg atttattttg aggcattttc attctcctac gactgaggcc aaccctgctg
    30721 taaacggttc aaggcacgac gcatactgac gctgacattg ttttcggtta tatcgaggtg
    30781 taatgcaatc tgctttacct gccagccaag catatagcgc aagacgagca tttcgcgcag
    30841 gtcctcgggc aaagtggata atgtcttcca taggaccata gcttgttcac gggtgatgat
    30901 atcgatttcg ggcagcgatt tcgtgtccag cagtgattcg aggtcgatgt tttcttcttc
    30961 ggggcggacc ctgcgtcggc gtgacgaatc aatgatcagg ttgcgtgcga ttcgtaaaat
    31021 ccaaccgagt gcggcctgtt tgctgccagt gaagcggtgg cgggtggtcc atgcccggat
    31081 gaaggtttcg gctgtaatat cctctgcgtc ttgctgaggt ccgccgctta gaccatatac
    31141 atagcgaaat acggtcaggt ggtgtagctc atagagtttt gcaaaggcat cagcatccgt
    31201 gatggaatat tcctgttctt gccataacca gcgtttaatg ttttgcagta cgggttgttc
    31261 agtcaaacag acctccgata tgtgaaatac gcgcgttcac catataaaac gcagatgggc
    31321 tcaaaatctg acaaacgaat atacactgga ataattaaag gcttatggaa ttttgagtat
    31381 tgtgggggat gtgtcagtct gttttggggt gggtgacatg gtcaaggccg tttctttttt
    31441 ctggaaagtg gttggcccaa gccttgttta ttgtgatttt ttgcattaat aactggatga
    31501 cagtataatc aacaaaaata cattgctgtg aggtggattg tgaaacgaat tttcatttct
    31561 tatcgcagac aggatagcgg ggtctttatt ggaaggattt atgatcaact taaacttcat
    31621 tttggcgaac gcaatgtgtt tcgggatgta tacaatattc ccgctggcag tgatttccgt
    31681 gaagtattga ataaggaagt ggggcagtgt gacattttcc tggctgtgat cggacgccag
    31741 tgggtggata tctccagtac tcagggcagg cggcgtttgg atgaccccaa tgattttgtg
    31801 cgtattgaag ttgaattggc attaagtaac ccaaatatcc ttgtgatccc tgttttggtg
    31861 gacaatgctg aaatgcctga ggttgaagat ttgcctccgg gcttgcagcc tttgtgttat
    31921 cggaatgcgg tcaaagtgcg gacagatccc gatttccccc atgacatgca aatgttgttg
    31981 caacaaataa agcccgccaa gtctttcata tcaacccggg ttggctggtt gatgttggcc
    32041 ttgtccatcc tcctgatcgc gggattaagt tttttcccca tgttcaatgg agaaaaggac
    32101 attccatctc ccacagctgc acagactgat ataccgccga cacccactcc gctgtttgct
    32161 ccggttgaac ctgtggagcc tggtcaggtt atggtccttg tggctaacat ggagcaggtc
    32221 ggttcaacaa agcgggatgt gacccgtttt attgtagaag atcttgtcaa tcattttgag
    32281 tctgatctgt cgagtacgaa tatccgtatt cgggagtatg aacgatttat tacctccaat
    32341 gatgaagcgc tgcaattggc cgagcagaca caagcggtga ttgtggtttg gggacagtat
    32401 gataacgaca atgttacggt gaatattcaa cttggttccc tgcagtcccg acctgccata
    32461 gcacttgacc gttccctgct tgagcaggcc gtcaatattc gagtgaaact gacggatgaa
    32521 cgtcaggaga ccctggcata tcacatcttg agttctctta tccttataca ttgtgctgaa
    32581 aatgacatgg tcaaagcgat gagtttgatc atttcgtttg accttctgga tgcgcccggc
    32641 cctgagccta tcgggaattc gagtgctgcc catgtgcaaa aggcaatgaa ggcatttctt
    32701 tcggatcgaa aaacagcgtt ggatgagctg aatcagacgg ttgagcttgc tcctgataat
    32761 ccactgtttt acatgcttcg cgcaatactg tatcaaggca tgggggaatt tgccctaagt
    32821 aatcaagatc ttgataccgc actgttgctg gctcctgaag attggattct gccttaccat
    32881 cttaaaggcg gaataagcct tcttttgagc gatgttccaa atggcgtgga gttttataca
    32941 agggtgatcg agaaaactcc tgatagctgg ttcccttata accaacgtgg ctacttgtat
    33001 ttccttgcag gacagtatga tctggcgcgt agtgatattg agaaatccat tgcgctcaaa
    33061 ccggaggcag aatggccgta tatgtggggc gcattgattg ctttgcgtca ggggcgcatg
    33121 gaagatgcct cctttatgat gtcactcctg ttgcaggaac agccaaaccc aacctttatt
    33181 aatcgtttga tgaccatttt atatggggaa gccactgcaa aactgttggg cgaatcgatg
    33241 gctgcaatgg gacatctcac tttgggccag tatgatgtgg ctattcaaga tacggacggc
    33301 gttttggcgg tcatgcctgc ctatactgat atgtatatgt tgcgcggatt aagctattgc
    33361 aacctggata actatgtgga tgcagaggcc gcttatacac agggcctgac ccttgacccc
    33421 tcatttgccc ttttacattt gctgcgtgtg gaggtccgaa caaaacaggg gaatgtagcg
    33481 ggcgcaatgg aggatattga tgccttacaa cagaataatg aactcacagc taatttggct
    33541 ccctatcttg ctgcggctca gtctggggag ttttcttgta aacaattatt gagatccaaa
    33601 taggcgtaaa ggttttttgt tttacgaaaa aaaggtcaag atatccagta cggatatctt
    33661 gacctttaaa attcagcagt ttccttttgg gcgggtgagt ggttattact tttgggccat
    33721 gttcatgcaa aatattgaat gatattttct gcaatatgaa cgatcaacta tccaaaaacg
    33781 tgattaaaaa aattaaagca gattacaact tggaagaaat tccccgcgtg gccgaatatt
    33841 taggacaata cggcacggac aaacccgaga cagaacgtgt gcagcttggg atcttaaacg
    33901 tggcaaatgg ggatattccc aaactccaaa tgttggtgtc cgttgctctt accgattaca
    33961 gggacctgct cgtgcaggcg gaatacctta atgatcctta taatcttttg caaaccctgc
    34021 ttcgaaactt gcaagagagc aatacattta caacagagga agtgacagaa acgatgcgcg
    34081 ccacgggtgg aacgaactat cgaaaggctt ttgagttcct tatggtaatg atcccccagc
    34141 gcgggaagac cctgaacgct gagcaatatc aattaataga taaactcggc agggcgttga
    34201 aact
//
LOCUS       DAOQJC010000034        33966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_563_length_33966_cov_9.672555, whole genome shotgun sequence.
ACCESSION   DAOQJC010000034 DAOQJC010000000
VERSION     DAOQJC010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 33966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33966
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_563_length_33966_cov_9.672555"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..376
                     /gene="rpsJ"
                     /locus_tag="PLE39_06970"
     CDS             68..376
                     /gene="rpsJ"
                     /locus_tag="PLE39_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNE68103.1"
                     /translation="MAKQKIRIRLKAYDHRVLDQSAKRIVETAERTGAQVVGPVPLPS
                     KIERFTIRRSAFIDKDSHEHFEIRTHNRLIDVLDPDSKTIDMLMRLNLPAGVDIEIKI
                     "
     gene            561..1193
                     /gene="rplC"
                     /locus_tag="PLE39_06975"
     CDS             561..1193
                     /gene="rplC"
                     /locus_tag="PLE39_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNE68104.1"
                     /translation="MLKGLIGKKIGMTQIFDEQGVAYTVTLIEAGPCYVTQVRTPEKE
                     GYSSVQLGFGEVNPKRLTGGQLGHFKANEISPVRFLREFRAKDHGLKVGDKVTVVDAF
                     AVGERVDVVGVSKGKGFAGGVKRYHFHGMNATHGTSDRNRAPGSRGSGTTPGRVYKGA
                     RGAGHMGVDRVTVQNIKVVMVDAERNLLAINGAVPGGKGSLVMIKESRKQ"
     gene            1222..1851
                     /gene="rplD"
                     /locus_tag="PLE39_06980"
     CDS             1222..1851
                     /gene="rplD"
                     /locus_tag="PLE39_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNE68105.1"
                     /translation="MKVDVFDLKGQKVREMELPANVFEAPVNVDLMHQAYVRQMANAR
                     LGTHETKVRGEVRGGGRKPFKQKGTGRARQGSTNAAQWVGGGRIHTPHPRSYEQRMPR
                     KMRLAALRSVLSAKAADAGIVVVDDLDIKEPKTRVMAEALGNLVGSSTALVLMPVKDQ
                     SYDNVMRSASNIESAKILLASYLNVRDVLNFDKVVLPLKTIDALVAHLG"
     gene            1861..2166
                     /gene="rplW"
                     /locus_tag="PLE39_06985"
     CDS             1861..2166
                     /gene="rplW"
                     /locus_tag="PLE39_06985"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNE68106.1"
                     /translation="MTTIHDVLVRPLVTEKSSFQSGKLGQYVFVVADKATRTMVKDAV
                     ETLFDVTVARVNILNAPAKRGRRTRSRRLLVRRPSFKKAIVTLAEGSKSLEIFEGVQ"
     gene            2166..3002
                     /gene="rplB"
                     /locus_tag="PLE39_06990"
     CDS             2166..3002
                     /gene="rplB"
                     /locus_tag="PLE39_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNE68107.1"
                     /translation="MAVKKYKPVTPGMRDMTGYTFEEITKSTPERSLLVKKTARGGRN
                     AQGRVTVRHRGGGARRYIRIVDFKREKHGIPAKVAAVEYDPNRTARLALLFYADGEKR
                     YIISPLDLKVGDAVMSGPNAEIRPGNALPISNIPVGTLIHNIEVKEGKGAQLVRSAGG
                     AAQLLAKEGDFAQIRMPSGEVRLIHQVCYATIGQVGNLDHGNVKLGKAGRKRHLGIRP
                     TVRGTAMSPRDHPHGGGEGRQPVGLPGPKSPWGKPTLGKKTRRNKKTDQYIVRRRSKT
                     NR"
     gene            3025..3297
                     /gene="rpsS"
                     /locus_tag="PLE39_06995"
     CDS             3025..3297
                     /gene="rpsS"
                     /locus_tag="PLE39_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNE68108.1"
                     /translation="MSRSLKKGPYIEPKLLKRIEAMNQKNEKKVIRTWSRPSVIFPQM
                     VGHTIAVHDGRRHVPIYITENMVGHRLGEFAPTRTFRGHQTKEAAA"
     gene            3309..3656
                     /gene="rplV"
                     /locus_tag="PLE39_07000"
     CDS             3309..3656
                     /gene="rplV"
                     /locus_tag="PLE39_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNE68109.1"
                     /translation="MSDIHAKLSHLSLSAQKVRIVIDMVRGKSANEALEILRFVNKRA
                     ALPVQKLVASAVANAEENFGVSRDDLYVAKITADEAPTRKWRRFGARGRFKPILRRSS
                     HVTVVLRERGREA"
     gene            3666..4415
                     /gene="rpsC"
                     /locus_tag="PLE39_07005"
     CDS             3666..4415
                     /gene="rpsC"
                     /locus_tag="PLE39_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNE68110.1"
                     /translation="MGRKVHPVGFRLGINQPWGGRWFAEGSTYRQQLHQDLAIRSLLL
                     GKFSKGGAAASEVVRGLRKQMPDAVKDGKAGIARVDVERTPGKVRVSIHTAKPGILIG
                     RKGEGVKKIRQALETLAGKKVELDIKEISSPDTDAMLVANNIADQLERRIAYRRAMKR
                     AIQQAIKQGAEGIKILVGGRLGGAEMSRDVWLREGRVPLQTLRANLDFATTEALTTYG
                     QIGIKVWIYKGESAPEVEEKAEATEGVYVSE"
     gene            4447..4866
                     /gene="rplP"
                     /locus_tag="PLE39_07010"
     CDS             4447..4866
                     /gene="rplP"
                     /locus_tag="PLE39_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNE68111.1"
                     /translation="MLMPKRVKWRKQMRGRMRGKALRGAEVSFGEYGLQALEPGWVTA
                     RQIEAARRSIVRGMKRRGKVWIRIFPSKPFTHKPPETRMGSGKGNVEYYVAVVKPGRV
                     MFEVGGLPGDIAVAALKSAMYKFSIKTKVVGRHAEGE"
     gene            4869..5084
                     /gene="rpmC"
                     /locus_tag="PLE39_07015"
     CDS             4869..5084
                     /gene="rpmC"
                     /locus_tag="PLE39_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNE68112.1"
                     /translation="MKSMKIEDIRKMAAEEIRKKLTDTRDELMKLRFQQVSGQLTDTS
                     SLPALRRNIARMETVLRELENAVVEGA"
     gene            5084..5356
                     /gene="rpsQ"
                     /locus_tag="PLE39_07020"
     CDS             5084..5356
                     /gene="rpsQ"
                     /locus_tag="PLE39_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNE68113.1"
                     /translation="MNNRRRMTGVVTSNKMTKTVVVEITRKFRHPLYKKVVYSSARVK
                     AHDEIGCQIGDEVQIVETRPLSRDKRWAVETIVKRETRTADQGVGE"
     gene            5360..5731
                     /gene="rplN"
                     /locus_tag="PLE39_07025"
     CDS             5360..5731
                     /gene="rplN"
                     /locus_tag="PLE39_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNE68114.1"
                     /translation="MIQQESRLKIADNTGAREILVIQVLGGSRRKYGAIGDIVVATVK
                     AAAPQGSVKKSEVVKAVIVRCKKEWRREDGSYIRFDDNAAVILDADGENPRGTRIFGP
                     VARELRDKGYMKIVSLAPEVL"
     gene            5741..6070
                     /gene="rplX"
                     /locus_tag="PLE39_07030"
     CDS             5741..6070
                     /gene="rplX"
                     /locus_tag="PLE39_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNE68115.1"
                     /translation="MKVKIRKGDTVEVISGRLEDKGKRGEVINVILSDRRVVVQGVNI
                     RTKHQKQVQAGNRQMNPGRIQFEGPVDISNVMLVCPKCSEATRVGIQRDDDGAHRVCK
                     NCDALID"
     gene            6088..6642
                     /gene="rplE"
                     /locus_tag="PLE39_07035"
     CDS             6088..6642
                     /gene="rplE"
                     /locus_tag="PLE39_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNE68116.1"
                     /translation="MNKMQELYNNEVAPALFKTFGFKNVMQVPRIEKVVLNIGLGEAM
                     DNPKALEAATADLMQITAQKPVQTKARKSIANFKLREGRLIGAKVTLRGPRMWAFIDR
                     LFNIALPRVRDFRGVSANAFDGRGNYTLGLKDQLVFPEIEYDKIDKLRGMEITIVTSA
                     QNDDHARALLRLLGMPFSSNKKDA"
     gene            6646..6828
                     /locus_tag="PLE39_07040"
     CDS             6646..6828
                     /locus_tag="PLE39_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNE68117.1"
                     /translation="MAKKCMQYRELRRRHAVQVRNRCQRCGRPRGFIRRFGLCRICFR
                     ELALTGKIPGVVKASW"
     gene            6848..7261
                     /gene="rpsH"
                     /locus_tag="PLE39_07045"
     CDS             6848..7261
                     /gene="rpsH"
                     /locus_tag="PLE39_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNE68118.1"
                     /translation="MSFTDPIADMLTRIRNAVLAGHAQVAMPNSKIKVEIAKILKDEG
                     FLEGFEVVEGEREAQKVLRVKLKYVGERRQRKAVLSGLERVSKPGRRIYTKKTDIPWV
                     LSGIGVAIISTPKGVMTGARARQLGVGGEIICKVW"
     gene            7272..7820
                     /gene="rplF"
                     /locus_tag="PLE39_07050"
     CDS             7272..7820
                     /gene="rplF"
                     /locus_tag="PLE39_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNE68119.1"
                     /translation="MSRIGRLPIDIPSGVQVSVNGLDVHVKGPKGEMKRSFSRMVGIK
                     MENNQLNITRNSEEPAERAMHGTARAVIANMIHGVSKGFEVVLQVEGVGYRAEMEGKN
                     LALFVGYSHPIKMEPPVGVTFETDAKTRQIKVSGYDKELIGQVAANIRKVRPPEPYHG
                     KGLRYLGERVRRKAGKAGKGAK"
     gene            7824..8189
                     /gene="rplR"
                     /locus_tag="PLE39_07055"
     CDS             7824..8189
                     /gene="rplR"
                     /locus_tag="PLE39_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNE68120.1"
                     /translation="MANKSRSLARERRHGRVRKHVYGTSDRPRLNVFKSLSGIYAQII
                     DDNEGNTLVSASTVDKELREKMKGLKKTEQAKAIGKAVAERAKSKGISAIVFDRGGFR
                     YVGRIKALADGAREGGLQF"
     gene            8235..8783
                     /gene="rpsE"
                     /locus_tag="PLE39_07060"
     CDS             8235..8783
                     /gene="rpsE"
                     /locus_tag="PLE39_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNE68121.1"
                     /translation="MAEKQFTEKQQFETQDAFEERVIEIARVAKVVKGGRRFRFRVTV
                     VVGDKNGTIGMGIGKANAVPDAMRKASERARKDLRVVNLSNTTIPHESIGKVAGAKVF
                     LKPASAGTGVIAAGGVRAVLELAGVRDILTKSMGSANVLNVVMATFGALEGLRSPANE
                     AARRGKRAEELVPFWERRKQHA"
     gene            8776..8973
                     /gene="rpmD"
                     /locus_tag="PLE39_07065"
     CDS             8776..8973
                     /gene="rpmD"
                     /locus_tag="PLE39_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNE68122.1"
                     /translation="MPKQTTSGKTLRVTWVKSDIGFPKDQKATLKALGLRRLHQTVEH
                     KDTPALRGMLNKVVHLLKIEE"
     gene            8982..9437
                     /gene="rplO"
                     /locus_tag="PLE39_07070"
     CDS             8982..9437
                     /gene="rplO"
                     /locus_tag="PLE39_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNE68123.1"
                     /translation="MKLHDLVPNAGSKKNRKRVGRGISAGQGKTAGRGTKGAGARSGE
                     GGHAYRQGGNLPFFRRLPFIRGEGFTPPNQVFYNEVNLDQLAQAFQANADVTPESLEQ
                     AHLLRDSRNPIVVLGRGEMTVALKVRVHRVSAGAKAKIEKAGGSVELIA"
     gene            9458..10846
                     /gene="secY"
                     /locus_tag="PLE39_07075"
     CDS             9458..10846
                     /gene="secY"
                     /locus_tag="PLE39_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNE68124.1"
                     /translation="MKSTFSGWHYLWKSDDIRRKLIVTFAILAIYRLAANVPAPGVNF
                     DVLAQFRASSAGSGGFLGFLDLLSGGTVSNFSLLSMGVYPYITAQIILQLLIPIIPAL
                     QRRMEEDPREARRWQEKWTYYLAVPMAALSAIGQINIFNSLSAQAIGQTILPFGLFSS
                     DLWLPSLTVLLSMTAGTMFAIWLGELISEYGIRGQGLSLVIFAGIVARMPANLVSLLS
                     DESTRWFMLIFTIIIIILTVFAIVYVQQGRRNVPVMYPGRRMGNRMSMPVKGTLPLMV
                     NLSGMIPLIFASAILQFPAIIASYFTSSENAGVAKFFTDVQNFFGATSTTTGNWGYWL
                     LYFFMVVVFTFFYTAVLFDQQNYGENLKKVGATVPGVHTGAPTQKYLSTVQRRITLVG
                     AVFLGVVAIMPFLLGLLLRALSLNAASSQAGLFLVSSSGLLIVVGVVRDTFQNIDAEL
                     KLHGYKDSLLVR"
     gene            10856..11506
                     /locus_tag="PLE39_07080"
     CDS             10856..11506
                     /locus_tag="PLE39_07080"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNE68125.1"
                     /translation="MTTFIVLLGPPGVGKGTQAKILADATGLAHISSGDLFRENLKNQ
                     TDLGKLAKSYMDKGELVPDDVTINMIRDRLKRDDCKSGAILDGFPRTPAQADALEKML
                     SEFNGHVDSVPYITAAEDILIERTGGRFTCRASGHIYHEKFNPPSKVGICDVDGSELY
                     QRDDDKKETVTKRIRVYLDQTMPLVEYYRTRGRLVEIDGDQPVEQVTQRLLAALKK"
     gene            11519..12334
                     /gene="map"
                     /locus_tag="PLE39_07085"
     CDS             11519..12334
                     /gene="map"
                     /locus_tag="PLE39_07085"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNE68126.1"
                     /translation="MQSDLLALLKCRFYSMSWERQIKVKSPAELKIMREAGHINASVL
                     AAVRELLQPGVTTADLNAAAEEVLKSHGCVSPFKNYGHPPFPASITVSINDEMVHGIP
                     HPKRKLKDGDIVSIDCGTTYNGFVADSAFTAGVGEISPQATKLLEITENALYAGIDKM
                     RKGNRTGDVSAAIQKYVEDNGFHVTREYTGHGVGRKMHEGPQVPNIGIPGTGLQLRPG
                     LTIALEPMVLVGTHETRVLPDQWTVVSADGSLTAHFEHTVAVTEGEPLILTVL"
     gene            12435..12548
                     /gene="rpmJ"
                     /locus_tag="PLE39_07090"
     CDS             12435..12548
                     /gene="rpmJ"
                     /locus_tag="PLE39_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNE68127.1"
                     /translation="MKVSPSIRKRCAKCRIIRRKGIVYIICENPKHKQRQG"
     gene            12560..12946
                     /gene="rpsM"
                     /locus_tag="PLE39_07095"
     CDS             12560..12946
                     /gene="rpsM"
                     /locus_tag="PLE39_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNE68128.1"
                     /translation="MARIEGVDLPRNKRVEVGLTYLYGIGPTRAQAILANTKVNPDTR
                     IKDLSEADVAAIRDYISKHYKVEGDLRREVQMSIKRLIEIGSYRGLRHRRNLPVRGQR
                     TRTNARIRKGTKKTVAGRGRRKGAKK"
     gene            12972..13376
                     /gene="rpsK"
                     /locus_tag="PLE39_07100"
     CDS             12972..13376
                     /gene="rpsK"
                     /locus_tag="PLE39_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNE68129.1"
                     /translation="MAQSVRSTRRPSGAKKGKRTLSYGQVHIFASFNNTIVTVTDTEG
                     NTVSWGSAGSAGFKGSRKSTPFAARLAAEQALKAAQQLGLQDVDLFVKGPGPGRENAI
                     RAVQSMGLKVRSISDITPVPHNGCRPPKKRRV"
     gene            13397..14062
                     /gene="rpsD"
                     /locus_tag="PLE39_07105"
     CDS             13397..14062
                     /gene="rpsD"
                     /locus_tag="PLE39_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNE68130.1"
                     /translation="MAKTLSPVCKLCRREGEKLFLKGERCFTPKCSFEKRDFAPGQHG
                     RTAGRGGGKGKGMGTGRASDFARQLRAKQKARRIYGVMERQFRRYYDTAKSGQSLTGL
                     TLLQILESRLDNVVFRLGFASSRSQARLLVAHGHFKVNGRRTDVPSMLLSEGDVVSVR
                     EGSSKLTYFKDLSAYTEKRTAPSWLNRDIKAMAGTLARMPERAEIDGSLDEQLIVEYY
                     SRR"
     gene            14160..15110
                     /locus_tag="PLE39_07110"
     CDS             14160..15110
                     /locus_tag="PLE39_07110"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNE68131.1"
                     /translation="MVMPKIEREAEARNYGKFTIGPLEGGYGVTLGNALRRVLLSSLE
                     GTAITSIRFADVLHEFSDVPGVREDVIQIMLQVKQIRIKMDGVDSARMHLEVRGEGTV
                     TAADIVTPAEVEIVNPDTYLFTVDGAKTKHDIEFVVERGRGYSPANERAGHMPIGELP
                     VDAIYSPVKRVNWEVSSARVGQSTNYDKLILEIWTDGTISPERALSSSARILIDHLRY
                     IAGINEEMLAVAVERESSGSRLSSELAETPVEALDLSVRVFNSLKRTGITNVGDVLDL
                     LEKGETAVMSIRNFGEKSLDELRQKMQEKGFMKDDTSSES"
     gene            15114..15491
                     /gene="rplQ"
                     /locus_tag="PLE39_07115"
     CDS             15114..15491
                     /gene="rplQ"
                     /locus_tag="PLE39_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNE68132.1"
                     /translation="MRHSVAGYILGRTKSSRIALRRNLIKQLFTHERIKTTEAKAAAV
                     RGDAERLITLARNSANGTDAEKLSARRLAISKLGDNQLIKRLFDEIAPRFATRPGGYT
                     RVTKLGPRLGDAAEMVVLELVEE"
     gene            15542..16288
                     /gene="truA"
                     /locus_tag="PLE39_07120"
     CDS             15542..16288
                     /gene="truA"
                     /locus_tag="PLE39_07120"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNE68133.1"
                     /translation="MARYKVILAYDGTGFTGSQRQANSRTVQGEFEKALRRLGWSDKS
                     IVMAGRTDTGVHATGQVAAFDFDWAHTEEKLLRALNSDLPSDLVIKSLITAPADFHPR
                     FDAASREYRYRLFCGTIRDPLREKYFWRVWPALDEDALKQAAAIFLGTHDFATMGSPT
                     TPKGTTVRTVTKAEWKRMPDDEWQFEVRADAFLYRMVRRLVFVQVSVAQGKAPLEKVR
                     KALDEKGKLPSGLAPAQGLTLVEVTYGSHD"
     gene            16311..16754
                     /gene="rplM"
                     /locus_tag="PLE39_07125"
     CDS             16311..16754
                     /gene="rplM"
                     /locus_tag="PLE39_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNE68134.1"
                     /translation="MQQKTFYPKAAEIQRDWVVIDAEGQTLGRLAARVAHVLLGKHKP
                     TFTPGVEMGDFVVVVNTERVSVTGTKTSSKLESKIYYSHSGYPGGLKSISLRDQLATH
                     PDRALRDAVWGMLPHNRMGRAVLKRLKIYAGPNHPHAVQTAKAAK"
     gene            16770..17162
                     /gene="rpsI"
                     /locus_tag="PLE39_07130"
     CDS             16770..17162
                     /gene="rpsI"
                     /locus_tag="PLE39_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNE68135.1"
                     /translation="MAQYYEGIGRRKASTARVRVSTGSGKFTVNEKEGAAFFTRMGDV
                     EDIMRPFSAVGQEAVKYDVSAVVNGGGTSGQTESVRLGIARALQLINAEWTAALRKKG
                     LLTRDARVKERKKPGLKKARKAPTYTKR"
     gene            17285..18388
                     /gene="mazG"
                     /locus_tag="PLE39_07135"
     CDS             17285..18388
                     /gene="mazG"
                     /locus_tag="PLE39_07135"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNE68136.1"
                     /translation="MDFSLVSQTFETLRIAPPSKLTLLEAHTLAPEFAHVPPFPPDIP
                     ALLTGIASRELALQVRTVLRTVYPQEHEVFVVEAGVAREHSLSELGTGEFSEQTCLYL
                     PSLAAGTSFESFAEIVAHLRAPDGCPWDKEQTHQTLRKHLLEEAYETLSAMDENNSDE
                     MREEFGDLLLQIVLNSQIANEAGEFTMNGVIKGIYDKIVRRHPHVFGDVKLDGVDGVL
                     QNWEKLKEQERKEKGEDGGKKKGLLDGVSAALPALTQAQEYQDRAARVGFDWPEIEGV
                     LDKIREEIEEIKKAQNLDEVTAELGDLFFVLVNLARWRKVDAESALRGTNQKFKKRFA
                     YVEQGAKQQGRNLSDMTLEEMDILWNEAKRVGI"
     gene            18482..19465
                     /locus_tag="PLE39_07140"
     CDS             18482..19465
                     /locus_tag="PLE39_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68137.1"
                     /translation="MESQLGTPSIPQKPKWDLPDVWAKSGHCPACGATNLKITHLPDY
                     PDYLSCAHCQVSFEVENGGRHIRLKYIPDQFEYADAMLRGRWVAAADLPAILRKPQPV
                     VKEADEPQLPKQPLSDAEIWKRAVGMYKLGNKPRMIQLTLMQSGVPQTQADAILEKLK
                     RLAEEDTRKMNQKFWILAGVALLLIFSLAGGWLYGSGNLAVLMGDATPVPTQSGPSAV
                     DLLLDLVPDEAKPAIIDLPKTSVDVGRGPGQASCPPTSGMAAELFGGDAAMWQQAGAF
                     AAWQMIDPINSATITVPTDMTAAYVDNRTRQFLSVHGPATIYNVNFLVISC"
     gene            complement(19473..20009)
                     /locus_tag="PLE39_07145"
     CDS             complement(19473..20009)
                     /locus_tag="PLE39_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE68138.1"
                     /translation="MPPSETDFTIRHINPADLQVFRELRLEALKNHPSAFGSDYEETL
                     LQPQSYWEKRLTFQVDEQALFFSEHHGQPIGMAGIVRNNSKKNHHSATIISVYVKPEW
                     RGRRIAEMLIHACLGWAAEHDVVIVKLAVVTDNLSAIHTYERCGFKIYGTEPKALRIN
                     GIYYNEHLMAVEIPHPSK"
     gene            complement(20036..20671)
                     /locus_tag="PLE39_07150"
     CDS             complement(20036..20671)
                     /locus_tag="PLE39_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68139.1"
                     /translation="MKKTALLFPNLCLILLLMAACSPKTTLPTLDPNIQIQQAVAETL
                     AAIPTATPYTPPTPFPSATPISLAGVFCEYQFCIGHPNNMAFYDHMAALNQLTPSAYN
                     NGFLAAYQIPTMVINFIWLQAPGTSDPQFLLETLLDDQADTKNGTFDVKLIRGMNVMY
                     VPITTTISEVPFGAIAAWTCGDRVFAWKVYTPQAETAGPLFEEALARFTCN"
     gene            complement(20737..22500)
                     /gene="nirK"
                     /locus_tag="PLE39_07155"
     CDS             complement(20737..22500)
                     /gene="nirK"
                     /locus_tag="PLE39_07155"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HNE68140.1"
                     /translation="MKTFLKFLAAAIFLGILVSACTGTSFQPVDKEYVLTTDLREGRL
                     VFLGVSDEINGLENPTLSARPGSTITVTLINGGEGQHNLTFPEVQASTAVLTEKGEEA
                     SVTFTVPNTHGEMEYYDDVANHAELGMRGKLMVSETDQPMPVAAEQSNGDPQVLAAFQ
                     KGACGSCHAIDGIPNAVGVIAPNLSDINTAAEEHLKSDAYTGTATTTEEYIRESIADP
                     NLFVAPTCPTGACAPNVMPATLKDALTEEEINSIVNYLSGLPEGAYSTSTEAAAPQAP
                     TTGADVVRDPTDLPAPLAVREPQTVRVDLETIEVEGQLADGTTFTYWTFNGAVPGPFI
                     RVRVGDTIEVHLKNSGSSTMNHSVDFHAVTGPGGGAAVTQTEPGKETTFTAKAINPGL
                     YVYHCATPMVANHIANGMYGLILVEPEEGLPPVDREFYVMQGDIYTTGAYGDHGMQTT
                     DVTKLLNEDPEYVVFNGAVGALTDQKPLKANVGETIRIFFGVGGPNLTSSFHVIGEIF
                     DRVFDQASITSAPLTDVQTTLVPPGGATMVEFKLEVPGRFILVDHALSRLQRGLAGFL
                     IVEGDPNPEIFDGTPAPGSGH"
     gene            complement(22514..22726)
                     /locus_tag="PLE39_07160"
     CDS             complement(22514..22726)
                     /locus_tag="PLE39_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68141.1"
                     /translation="MNESFEISEASVEDILRSIPGAARLFLNWRTGCVGCGFARFCTW
                     KDVVKTYQLDGQKALEELKQANVQNP"
     gene            complement(22803..23501)
                     /locus_tag="PLE39_07165"
     CDS             complement(22803..23501)
                     /locus_tag="PLE39_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE68142.1"
                     /translation="MNLSPKDLKQVIVFQNATDDDLRLILQNSVERSMEESGFFFFHG
                     DPAHYLYILTSGQVKLMQNSVNGQQVNLRIIYPWQMFGALGAVRAEATYPASAQALEN
                     STALAIKSEFLHEMIQTRPQLSFDLMTLMTTYIQEMQARYRELATERVEQRVANALVR
                     LAGQAGIRSEKDAAIELSFSRQDVAEMTGTTLYTVSRLFSDWERQGIITTGRERIRII
                     KPHDLVRIADGLEK"
     gene            23590..24636
                     /locus_tag="PLE39_07170"
     CDS             23590..24636
                     /locus_tag="PLE39_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNE68143.1"
                     /translation="MKKIFVVLGVVFMMAVLAIWKYQQVELYQFHGSVFREPKLAAEI
                     VLRSASGPVRLADYRGKITLLYFGYTSCPDVCPTTLANLKLALENLAPADASQVQVIF
                     VSVDPDRDTPERLGKYVQLFNSGFVGATGTRQEIDLMTDAFDVYYKINNSESKENYSV
                     DHSGFVTVIDRHGYAVMSWPHGTAPRDVSEDLQYLIEHDMPVSAQILAGPTYTPVVCA
                     VTLAPAHIQGGEWLYQHHCAQCHGADLGGNASWQVELADGTRLPPPLDGNGKAWQYSE
                     TELIEIIKNGRNLDKPLFMPAFKTTLADWEISFILTYLKSHWSINQLNYQHGFMTLTP
                     QATPSTLPTNTALP"
     gene            24793..25062
                     /locus_tag="PLE39_07175"
     CDS             24793..25062
                     /locus_tag="PLE39_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNE68144.1"
                     /translation="MENDNTLRRLKTVEGHLRGVIRMVEEDAYCIDVIRQIQAIEAAL
                     NKVSSKILEDHLNSCVITAIQGSDKKERERVLKEITEVFEMSTKV"
     gene            25154..25372
                     /locus_tag="PLE39_07180"
     CDS             25154..25372
                     /locus_tag="PLE39_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNE68145.1"
                     /translation="MTTVTYTVPAISCGHCTRTIETEVADLQGVQTVKAEIESKKVTI
                     TFDAPADETKIKALLAEINYPAEGLLTL"
     gene            25430..27901
                     /locus_tag="PLE39_07185"
     CDS             25430..27901
                     /locus_tag="PLE39_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HNE68146.1"
                     /translation="MTDTKQLTLPITGMTCANCVATVERNLKKLDGVQSAVVNLSSER
                     ATVDFDAAKLGLTDVIARVNRAGYGVATGEADLIIKRLSDGNDAARLEKALVKLEGVL
                     EAQVTFANEKARVKYIPTIITQAELRRAVSSAGFEALELGGEAEDAEALAREHEIKEQ
                     RRLLIIGLVFTVPLFLLSMSKDFGLLPDFFYTGTTMDGMRDAQPWFGWLMLVLALPVQ
                     FYVGWQYYVGAFKALRNRSANMDVLIVMGSSAAFFYSLPITFGWLMGHVYYETAAVII
                     TLIKLGKFLEARAKGRTSEAIKKLMGLRAKTARVIRDGVEAEVSIDDVRVGDMVLVKP
                     GEKIPVDGAVVEGRSAVDESMLTGESLPVEKKPGDAVIGATLNKLGMLKFEATKVGRE
                     TALAQIIKLVEDAQGSKAPIQKMADQVSAVFVPIVIGIAFLTFLAWYFFGPALPINSD
                     IDNFTRALIYMVAVLVIACPCAMGLATPTAIMVGTGKGAEAGILFRNSEALERAGRVS
                     VVVLDKTGTITKGQPAVTDIVTNNESPMTGDELLRLAASVEKGSEHPLGESIWAEATT
                     RGLVLVEPAGFKAEAGHGVQAEVEGRSVMVGNRRMFEARGIQLGNLESEVNRLQSEAK
                     TAMLVAVDGLAVGIIAVADTIKDTSKDAITNLHEMGLKVAMITGDNQKTADAIAKQVG
                     IDMVLAEVLPEGKSAEVKKLQSDQSPTTHVVAMVGDGVNDAPALAQADVGLAIGTGTD
                     VAMAAAPVTLMSGDLRGVSRAILLSRKTLSTIKQNLFWAFIYNVILIPAAALGYLNPM
                     LAAGAMAFSSVFVVTNSLRLRSVKI"
     gene            27953..28456
                     /locus_tag="PLE39_07190"
     CDS             27953..28456
                     /locus_tag="PLE39_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68147.1"
                     /translation="MKKLTLLLLAVLFLTACAPKAAEMPKAELPAGKAWADGKEIYFI
                     HTEASDAGVAEKLTNMMKSPVVLVPSLANVPAESLANVYVFTNGLAGTGPFGFQADVF
                     DNPPGTDGYTPLRSLHLITWTDETKARELTSAADVLAAEAAGELTIEQPGVVINMPFV
                     VWDGGKR"
     gene            28492..29664
                     /locus_tag="PLE39_07195"
     CDS             28492..29664
                     /locus_tag="PLE39_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNE68148.1"
                     /translation="MKKNKNKPFDPVIIITVGVTFLAVAFMLLVKPQSLAGDSSAMPQ
                     LMVAFVTGLTTGGLSCLAVQGGLLASSLAHQIEQDYVAGQATKKAASPRTNSALPILL
                     FLISKIAAYTLLGALLGILGSYLTLSPLTRAMLMILIGIFMIGNALRMFNVHPIFRYF
                     SIEPPSSIKRYIRKTAKGTDTATPVFLGALTVLIPCGVTQAMMATALGTGSAGMGAAL
                     MFAFTLGTSPVFFIVAYLTTELGSKLEKFFMRFVAVVVLILGFVTLDGGLNLMGSPLS
                     FQNLTRNLLPSENNSTTQAVSSLPAAEGEIVLEVQNSGYFPTTLKAPAGKELTLNLIT
                     DQTYSCARDFVIPSLDYYQLLPETGTTQVNIPAQEQGSTLFFTCSMGMYTGQIVFE"
     gene            29683..29895
                     /locus_tag="PLE39_07200"
     CDS             29683..29895
                     /locus_tag="PLE39_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNE68149.1"
                     /translation="MLKKTFKVEDMTCANCAMKLEALEDTLEGVKEINASYHRLEMVI
                     EYDEAKLTDEQIVAAVKKKGYTAVVK"
     gene            30042..30425
                     /locus_tag="PLE39_07205"
     CDS             30042..30425
                     /locus_tag="PLE39_07205"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HNE68150.1"
                     /translation="MPKYYTLHEANKTLTIIEPMLQEMMEIAMKIRENQPELWDLVRK
                     SAGNGGNPHLSRLLVEFDHLDALFHQLQDMGVVVKDLIIGLIDFTALHNGREVYLCWK
                     YGEGAIQFWHEIEAGFAGRQRIDWE"
     gene            30494..31327
                     /locus_tag="PLE39_07210"
     CDS             30494..31327
                     /locus_tag="PLE39_07210"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNE68151.1"
                     /translation="MTVSTIGRYQVESEIAQGGMGVIFLARDPYIQRQVVVKVLMYSR
                     TLEDVYREFFQQEAEVIAALEHPCIVPIYDFGWHGEQPYIVMRHMSGGSLDDHLRKAE
                     VKLTDMAHILKRVSDALDAAHARNIIHRDVKPSNILFDSTGEAFLSDFGIAKSQKISD
                     EDGEEYMVGTPAYMSPEQIKGGRLDGRSDVYAMGVVLYRLLTGQLPFSSNSTTGLINA
                     HVNMPVPDVRLVKANIPAVWQEVISKAMAKDPSDRYATAGDFARDVNEVVSGKWFLRK
                     L"
     gene            31507..32406
                     /gene="rarD"
                     /locus_tag="PLE39_07215"
     CDS             31507..32406
                     /gene="rarD"
                     /locus_tag="PLE39_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HNE68152.1"
                     /translation="MKKGILYGIGAYLLWGFFPIYWKFLHVVPALQVIGHRIAWSFLV
                     LGVYILLTGQWRDFRKVASNAKTIGIYAIAGVLLSINWLIYVWGVNAGYIVETSLGYF
                     INPLISVLLGVLFLRERLRPAQWLPVFIAAVGVFYLTFVYGRLPWIALSLAFSFGFYG
                     LVKKLAPLGSLYGLTLETGIVFPAALVYLIIAQAGGSGAFMHDGLQVDLFLMGAGIVT
                     TIPLLMFASAAKEIPLSMVGILQYIAPTLQFLIGVFLYHEPFDQSHLIGFGIVWVALV
                     IFWVENYIANRNPVEPIPELGEG"
     gene            32569..33762
                     /locus_tag="PLE39_07220"
     CDS             32569..33762
                     /locus_tag="PLE39_07220"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNE68153.1"
                     /translation="MANKKGVIGILTGGGDVPGLNPAIRAVTIRALREGYQVVGIRRG
                     WAGLVDIIRDKNADNSNNYQFLSEEIVNRAGRTGGTFLHTSRTKPSSVKKASVPEHLQ
                     GQYAEEKNDLTPEVLKNLAFLGVDYLIPIGGDDTLSYGVRLYKEGVKVIAIPKTMDND
                     VPGTDYCIGFSTCVTRTISLTNSLRTIAGSHERFLVLEVFGRYAGFTAMLPTMAGAAN
                     RCVIPEYKFNIEHLTELMSKDRDANPSRYSVLLISEGAMYEGGEMVFEDRTTDAYGHA
                     KLGGIGDLVSSQIKEISPKFNKGKTVDVVNQKLGYMVRGGDPDAIDSIVPMAFGNLAL
                     DLLLKGVHGRLVVLKNGRYDNMPLDVVTASKKVVEVKHFYDTDRYRPIYENFEMKPLF
                     IMTSE"
BASE COUNT         8150 a         8291 c         9202 g         8323 t
ORIGIN      
        1 ttcgtgaagg cggtctcacc gtcggcgcgg gtgtcgttac caagatcatc gagtaggtga
       61 gatgatgatg gctaaacaga aaatccgaat tcgcctcaaa gcatacgatc atcgcgtgct
      121 tgaccagtct gctaagcgta ttgttgaaac cgccgagcgt acgggagccc aagttgttgg
      181 gcccgtaccc ttgccaagta agattgaacg ctttacaata cgtcgctctg cattcattga
      241 taaggattcg cacgagcact ttgaaatccg gacccataac cgtctaattg atgttctgga
      301 tccagattcc aagacgattg atatgttgat gcgtttgaac ctgcctgcag gtgttgatat
      361 cgagattaag atctaatatt ttgtcagtaa ctgtagaata atttctacag attatttgag
      421 acagcgcaag agtaggtctg tgttgtagaa ccgacacaca ccgcttcgca ccagaccttc
      481 tgactgcgct gcacggagat gatgcggtcg ttgccccgac tggcagtctc gtcaatatct
      541 tctaaaggag taaattgagt atgttgaaag ggctaattgg taagaagatc ggcatgaccc
      601 agatcttcga tgagcagggc gttgcctata cggtaaccct catcgaagct gggccatgct
      661 acgttaccca ggtacgcaca ccggagaagg aaggttattc atcggtacag ctgggttttg
      721 gcgaagtgaa tcccaaacgc cttacgggtg gacagttggg tcattttaaa gccaatgaaa
      781 tttcccctgt gcgctttctg cgcgaatttc gagcaaaaga tcatggtttg aaagtcggcg
      841 acaaggttac agttgtcgac gcttttgccg ttggcgagcg tgttgatgtg gttggcgtta
      901 gcaaaggcaa gggctttgcc ggtggcgtca aacgctatca ctttcacggt atgaacgcaa
      961 cccacggtac atctgaccgt aatcgcgccc ctggttctcg tggttcggga actacaccag
     1021 gccgtgttta caaaggtgca cgcggcgctg gtcacatggg cgtggatcgt gtcaccgttc
     1081 agaacatcaa agttgtcatg gtcgatgcgg aacgcaatct gcttgccatt aatggtgcag
     1141 ttccgggcgg caagggtagt ctcgtcatga tcaaggaatc gcgcaagcaa tagcgcgtag
     1201 gaaacgggag ccagattaac catgaaagta gatgtttttg atttgaaggg ccagaaagtt
     1261 cgcgaaatgg aattgcccgc gaatgttttt gaagcccctg taaatgtcga cctgatgcat
     1321 caggcatatg tccgtcagat ggcaaatgcc cgtcttggca cacatgaaac aaaggttcgc
     1381 ggagaagtcc gcggtggtgg tcgcaagccg ttcaagcaaa agggaaccgg tcgtgctcgt
     1441 caaggttcca ctaacgctgc tcagtgggta ggtggtggtc gtattcatac cccccatcct
     1501 cgcagctacg aacagcgtat gcccagaaaa atgcgccttg ccgcccttcg ctctgtttta
     1561 tctgccaaag cggccgatgc cggtattgtt gtagtggacg atctcgatat caaagaaccc
     1621 aagacgcgtg tcatggctga agcgttgggt aatcttgtcg gttcatcaac cgcgttggtt
     1681 ttgatgcccg tcaaggatca gagctatgac aatgtcatgc gttctgccag caatattgag
     1741 agcgccaaga tcttactcgc aagctacttg aatgtgcgtg atgtccttaa tttcgacaag
     1801 gtcgtcctgc cgctcaaaac aattgatgcg ctcgtggcgc acctcggata ggagagagac
     1861 atgaccacga ttcatgatgt cctcgtccgc cctttggtga ctgaaaaatc cagtttccag
     1921 tcgggcaaac ttggtcaata cgtttttgtt gtcgctgaca aggcgacccg caccatggta
     1981 aaggatgcag tggaaaccct gtttgatgtt actgtagcgc gtgtaaacat tctgaatgca
     2041 cctgccaaac gtggccgccg aactcgtagc cgccgcttgc tggtgcgccg tccttcgttc
     2101 aagaaagcca ttgttacttt ggccgaagga agcaagtccc ttgaaatctt tgaaggggtg
     2161 caataatggc agttaaaaag tacaaaccag taacccctgg catgcgcgac atgaccgggt
     2221 atacctttga ggaaattacc aaaagcactc ctgaacgttc ccttttggta aaaaagacag
     2281 cccgtggtgg tcgtaacgcc cagggccgcg tcactgttcg gcatcgtggc ggcggcgctc
     2341 gtcgctacat ccgtatcgta gatttcaaac gcgaaaaaca tggcattccc gccaaagttg
     2401 ctgctgttga atatgatccg aaccgcaccg cgcgtcttgc attgcttttt tatgcggatg
     2461 gtgaaaaacg ctacatcatt tcgccccttg acctgaaggt cggtgatgcg gtcatgtctg
     2521 gccccaatgc cgaaattcgc cccggcaacg cccttccaat tagcaacatt cctgtgggta
     2581 ctttgatcca caatatcgaa gttaaggaag gcaagggtgc ccagcttgtc cgttcagccg
     2641 gtggagctgc tcaattgctt gcaaaagaag gcgactttgc gcaaatccgc atgccctctg
     2701 gtgaagttcg cttgatccat caggtttgtt atgcaaccat cggtcaggtt ggcaatcttg
     2761 atcacggtaa cgtcaaactc ggcaaagccg gacgcaaacg ccacctgggt atccgcccaa
     2821 ccgttcgtgg aactgcaatg agcccccgcg accatcctca tggtggtggt gaaggccgcc
     2881 agcctgttgg tctccctggt cctaagagtc cgtggggtaa acccactctc ggcaagaaga
     2941 cccgccgaaa caagaagact gaccagtaca tcgtgcgccg tcgcagtaag acgaaccgct
     3001 aagaattaga cgaggtacgt atatatgtca cgatcattaa aaaaaggtcc ttacattgag
     3061 ccaaaactgc tcaaacgtat tgaggccatg aaccagaaaa atgaaaagaa agtcatccgc
     3121 acttggagcc gcccatctgt gattttccct cagatggttg gacacaccat tgcggttcat
     3181 gacggacgcc gccatgtacc gatctacatt accgaaaaca tggtcggtca ccgcttgggc
     3241 gaattcgccc cgacacgtac cttccgcggt caccagacca aagaagctgc ggcataggaa
     3301 gttgagccat gagcgatatt cacgcaaaac tcagccacct ttccctctct gcgcagaagg
     3361 tccgcatcgt aattgacatg gtgcgcggca agagcgccaa tgaagccctg gaaatccttc
     3421 gcttcgtaaa caaacgtgcc gcattgcctg tgcaaaaact ggtcgcttct gcagttgcca
     3481 atgctgaaga aaactttggc gtcagccgcg acgacctgta tgttgccaag atcacagccg
     3541 atgaagctcc cactcgcaag tggcgtcgct tcggtgcccg tggccgtttc aaaccgatat
     3601 tacgccgtag ttcacatgta actgttgtgt tgcgtgagcg cggacgcgaa gcgtaaggag
     3661 attttatggg tcgtaaagta catcctgttg gttttcgttt agggatcaat cagccctggg
     3721 gtggtcgttg gttcgctgaa ggtagcacct atcgtcagca actacaccag gatctggcca
     3781 ttcgctccct gttacttggt aaattctcca agggtggcgc ggcagctagt gaagttgtgc
     3841 gcggtctccg caagcagatg cccgatgctg tcaaagacgg caaagcaggt attgcccgtg
     3901 tagacgttga gcgcaccccc ggcaaggtcc gcgtttcaat ccacaccgcc aaacccggca
     3961 ttttgattgg ccgcaaaggc gaaggtgtga aaaagatccg ccaggctttg gaaacccttg
     4021 ctggcaaaaa ggtcgagctg gatatcaaag agatttcttc ccccgacacc gacgccatgc
     4081 tggtggcaaa taacattgct gatcagcttg aacgccgcat tgcatatcgc cgcgccatga
     4141 agcgcgccat tcagcaggcc atcaaacaag gcgctgaagg catcaagatt ttggtcggtg
     4201 gtcgcttggg cggcgcggaa atgtcccgcg atgtgtggtt gcgtgaaggc cgcgtgcctt
     4261 tgcagaccct gcgcgccaat cttgatttcg ccactacaga agcattgacc acctatggtc
     4321 agatcggtat caaggtctgg atctacaagg gtgagtctgc acctgaagtt gaagaaaaag
     4381 ctgaagcgac ggaaggcgtg tacgtcagcg agtaatcgcg ctgtcgcatg agatgaggta
     4441 attgatatgt taatgccgaa gcgtgtaaag tggcgcaagc agatgcgcgg tcgtatgagg
     4501 ggcaaggctc tccgcggtgc ggaagtttcc tttggcgaat atggtctgca ggcgctggaa
     4561 ccgggctggg tcaccgcccg tcaaattgaa gcggcccgcc gctccattgt ccgtggaatg
     4621 aagcgccgtg gaaaggtctg gattcgcatt ttcccatcca agccttttac ccacaagccg
     4681 cccgagactc gtatgggttc cggtaaagga aatgtggagt actacgtggc agtagttaaa
     4741 cctggccgcg ttatgttcga agtgggtggc ttgcctggtg atattgctgt ggccgccttg
     4801 aagagcgcaa tgtataaatt ctccattaag accaaggtcg tgggtcgcca cgcggaagga
     4861 gaataaagat gaagtctatg aaaatagaag acatccgcaa aatggctgcg gaggaaatcc
     4921 gcaaaaaact cacagatacg cgcgatgaat taatgaaact gcgctttcag caggtgagtg
     4981 gtcagttgac cgataccagc agcctgccag ccctgcgccg caacatcgcc cgcatggaaa
     5041 cggtcttgcg cgagcttgag aacgctgttg tggaaggtgc ataatgaata atcgtcgtcg
     5101 tatgactggt gtagtgacca gcaataaaat gaccaagact gttgtggtgg agatcacccg
     5161 caagtttcgt caccctcttt ataagaaggt ggtgtactca agcgcacgtg taaaagcgca
     5221 tgatgaaatc ggctgccaga ttggtgatga ggttcagatc gtggaaaccc gtcccttgtc
     5281 tcgtgataag cgctgggcgg ttgaaaccat cgtcaagcgt gagacacgca ccgctgatca
     5341 aggtgtagga gaataagcca tgatccagca agagtctcgt ttaaagattg ccgataatac
     5401 cggcgcccgc gaaatacttg tcattcaggt gttgggcggt tccagacgca agtatggagc
     5461 cattggtgat attgtggtgg caacagttaa ggctgccgcc ccccaaggct ctgtgaagaa
     5521 gagtgaagtt gtaaaggctg tcattgtgcg ctgcaagaag gaatggcgtc gcgaagatgg
     5581 ctcatacatc cgctttgatg ataacgccgc ggtcattctt gacgctgatg gcgaaaaccc
     5641 gaggggcaca cgcatctttg gacctgtggc acgcgaattg cgtgacaagg gttacatgaa
     5701 gattgtgtcg ctggccccgg aagtgctgta ggagcgaaga atgaaagtta agattcgtaa
     5761 aggcgataca gtagaagtga ttagcggccg ccttgaagat aaaggcaaac gcggtgaggt
     5821 tattaacgtg atccttagtg atcgccgcgt ggtcgtgcag ggtgtgaaca tccgcaccaa
     5881 gcatcagaag caggtccagg caggtaatcg ccaaatgaac cctggccgta ttcagttcga
     5941 gggaccggta gacatctcaa atgtgatgct ggtttgcccc aaatgcagtg aagccacccg
     6001 cgttggaatt cagcgcgatg atgatggtgc ccaccgcgtt tgcaagaact gcgatgcttt
     6061 gatcgattag gccgtggagc agataatatg aacaagatgc aagaactcta taacaatgaa
     6121 gtagccccgg ccctgttcaa gacgttcggt ttcaagaatg tgatgcaggt cccccgcatc
     6181 gaaaaagttg tgttgaacat tggtctcggc gaagcgatgg ataacccgaa agctttggaa
     6241 gccgccaccg ctgatttgat gcagatcacc gcccagaagc ccgtgcagac aaaggcgcgc
     6301 aagagcattg cgaacttcaa gttgcgcgaa ggacgcctga tcggtgcgaa ggtcaccctg
     6361 cgtggacccc gcatgtgggc ttttattgac cgtcttttca acattgcatt gccgcgcgta
     6421 cgcgatttcc gcggtgtttc ggcgaacgct tttgatggtc gtggaaatta cacattgggt
     6481 ctcaaggatc agctcgtttt tcctgagatc gaatatgaca aaattgacaa attgcgtggt
     6541 atggaaatca ccattgtgac ttctgcccaa aatgatgatc acgcccgcgc attgttgcga
     6601 ttgcttggaa tgccgttcag cagcaacaag aaggatgctt aatctatggc aaagaaatgt
     6661 atgcaatatc gagaactgcg ccgaagacat gctgttcagg tgcgcaatcg ttgtcagcgt
     6721 tgtggtcgcc cgcgtggttt tattcgccgc tttggtctgt gccgtatttg tttccgtgaa
     6781 ttggcactga cgggcaaaat ccccggcgtc gtaaaggcat cctggtagcc ccaggtggag
     6841 gaagatcatg tcatttactg atccgatcgc tgatatgttg acccgcattc gcaatgctgt
     6901 gctggctggc catgctcagg ttgcaatgcc caactcaaag attaaagtag agatcgccaa
     6961 gatcctcaag gatgaaggct ttctcgaagg tttcgaagtg gtggaaggtg agcgtgaagc
     7021 ccagaaggtt ttacgcgtga agctgaaata tgttggcgaa cgtcgccagc gtaaggcggt
     7081 actttccggt cttgagcgcg taagcaagcc gggccgccgc atctacacca agaagacgga
     7141 cattccgtgg gtgctttctg gtattggcgt agccattatc tccactccca agggtgtcat
     7201 gaccggcgca cgtgcccgac agttgggtgt gggcggcgag atcatctgca aggtgtggta
     7261 ggaggtttat cgtgtctcgc attggtcgat tgcctattga tattcccagc ggcgtacagg
     7321 tgagcgtgaa cggtttggat gttcatgtga agggcccgaa gggtgagatg aaacgatcgt
     7381 tttcccgcat ggttggcatt aagatggaaa acaatcagtt gaacatcact cgcaactcgg
     7441 aagagcccgc cgaacgcgcc atgcatggta ctgcccgagc cgtgattgca aatatgattc
     7501 acggtgtcag caagggtttt gaggttgttc ttcaggtgga aggcgtgggt taccgcgctg
     7561 aaatggaagg caagaacctg gctctgtttg ttggttattc acacccgatc aaaatggaac
     7621 ccccggtggg tgtgacattt gaaacagatg caaaaacccg tcagattaaa gtttcgggtt
     7681 acgacaagga attaattggt caggttgcag cgaatattcg caaggttcgt ccccctgagc
     7741 cttatcacgg caagggtttg cgctacctcg gtgaacgtgt tcgccgcaaa gctggtaaag
     7801 ctggaaaagg agccaagtaa gctatggcta ataaaagtcg ctctctcgct cgcgaacgcc
     7861 gtcatggacg tgtacgcaag catgtttatg gaacttcaga tcgcccgcgc ttgaatgtgt
     7921 tcaagagcct ttctggaatc tatgcccaga tcattgatga taacgaggga aacaccctgg
     7981 tttcagcatc cacagttgat aaagaactgc gtgagaagat gaaggggttg aagaagaccg
     8041 aacaggccaa ggccattggt aaagcagtgg ctgaacgtgc caagtccaaa gggatttctg
     8101 caattgtgtt cgaccgcgga ggtttccgct atgttggacg cattaaggcc cttgctgacg
     8161 gtgcccgcga aggcggtttg caattctaga agttgtaaca acgggttccc gttgttgcgt
     8221 tactggagaa agatatggca gaaaagcaat ttactgaaaa gcagcaattt gaaacacagg
     8281 atgcctttga agagcgcgtt attgaaattg cccgcgtcgc taaagtagtc aagggtggcc
     8341 gtcgcttccg cttccgcgta acagtggttg tgggtgataa gaatggcacc attggtatgg
     8401 gcattggaaa ggcaaatgct gttccggatg ccatgcgtaa ggcatctgaa cgggctcgta
     8461 aagatttgcg tgtagtaaat ctttccaaca ccaccattcc gcacgaatcg atcggcaaag
     8521 ttgcaggtgc aaaagtgttc ctcaagcctg cctcggctgg tacgggcgta atcgccgctg
     8581 gtggcgtacg tgctgttttg gaactggccg gtgttcgtga tattctcacc aaatccatgg
     8641 gcagcgccaa cgttttgaac gtagtgatgg cgacctttgg tgctcttgaa ggtctgcgtt
     8701 cacctgcaaa tgaagcggcc cgccgtggca aacgtgccga agagttggta cccttctggg
     8761 agcggaggaa gcaacatgcc taagcaaacc acctctggca aaaccctgcg tgtgacttgg
     8821 gtgaaaagtg atattggctt tcccaaggat caaaaggcaa cccttaaggc gcttgggttg
     8881 cgcagattac atcaaactgt tgagcataag gatacacccg cgttgcgcgg catgttgaac
     8941 aaggttgtcc atttgctcaa gatcgaagag taggttgaat tatgaaactt cacgatctcg
     9001 tacccaatgc tgggtcaaaa aagaatcgaa aacgtgttgg tcgtggtatt tcggcagggc
     9061 agggtaaaac tgctggccga ggcaccaagg gtgcaggcgc acgttctggc gagggcggtc
     9121 acgcttatcg tcaaggtggt aacctgcctt tcttccgtcg gttgccgttt atccgcggtg
     9181 aaggttttac cccgcccaat caggttttct acaatgaagt gaaccttgat caacttgcac
     9241 aggccttcca ggccaatgct gatgtgacgc ccgaatccct cgaacaggcg catcttctcc
     9301 gcgattcccg caaccctatc gttgtgcttg gtcgtggtga gatgaccgtc gctttgaagg
     9361 ttcgcgtcca tcgcgtgagt gctggtgcca aagccaaaat tgaaaaggct ggcggctcgg
     9421 ttgagttgat cgcctaaccc attaaaggat actttgcatg aagagcactt tttcaggctg
     9481 gcattacctt tggaaatcgg atgatatccg acgcaagttg atcgttactt ttgctatttt
     9541 agcgatttat cgtcttgcgg cgaatgtccc tgcccctggt gttaattttg atgtattggc
     9601 ccaattccgg gcttcatctg cgggcagcgg aggcttcctt ggtttccttg atctactctc
     9661 gggcggaaca gtctccaact tttcactgtt gtcgatgggt gtgtacccat acattacagc
     9721 acagattatt ttgcagctgt tgattccaat cattcccgct ttacagcgca gaatggaaga
     9781 ggatccacgt gaggctcgcc gttggcagga aaagtggaca tattaccttg ccgtgccgat
     9841 ggctgctctt tccgcgattg gtcagatcaa tatttttaat tctctgtccg ctcaggcaat
     9901 tgggcagacg attctcccct ttggattgtt ttcatctgac ctttggcttc cttctctgac
     9961 agttttgctg tctatgacag ctgggaccat gtttgctatc tggctgggtg agttgatttc
    10021 agaatacggc attcgcggac agggactttc ccttgtcatc tttgcaggta tcgttgcacg
    10081 catgcccgcg aatcttgtct cccttctttc agatgagtca acccgttggt tcatgttgat
    10141 atttaccatc attatcatta tcttaacggt gttcgcaatt gtgtacgtgc agcagggacg
    10201 ccgtaatgtt ccggtaatgt atccaggccg ccgcatggga aatcgtatgt ccatgccggt
    10261 caagggaaca ctacctttga tggtgaattt gtcaggcatg atccccttga tttttgcaag
    10321 tgccattttg cagttcccag ccattatcgc aagctatttc actagcagcg aaaatgcggg
    10381 tgttgccaag ttttttactg acgttcagaa cttttttggc gctaccagca caaccactgg
    10441 aaattgggga tactggcttc tgtatttctt catggttgta gttttcacct tcttctacac
    10501 tgctgtattg tttgaccagc aaaactatgg tgaaaacctg aagaaggtgg gagcgactgt
    10561 tcctggcgta cacactggtg cgcccacaca gaagtatttg agcacagttc agcgacgtat
    10621 tactctggtt ggagccgtct tccttggtgt ggttgcaatt atgccatttc tccttggatt
    10681 gcttcttcga gcattgagtc tgaatgctgc cagttctcag gctggtttat tcctggtttc
    10741 ctcttcaggc ttgctcattg tggtcggtgt ggttcgagac accttccaaa acattgatgc
    10801 tgaacttaaa ttgcacggct ataaagattc attgcttgtc cggtaaggag ttgtgatgac
    10861 caccttcatc gtcctgcttg gtcctccagg tgttggtaaa gggactcagg ccaagatttt
    10921 ggctgatgca acaggcctgg cgcatatctc ttcgggagat ttgttccgcg aaaaccttaa
    10981 aaaccaaacc gatctgggta agctggcaaa atcctatatg gataagggcg aattggtacc
    11041 ggatgatgtg accatcaata tgattcgcga ccgcttgaag cgggatgact gcaaaagcgg
    11101 cgcgatcctt gatggtttcc cacgtacacc cgcgcaggct gatgcattgg aaaaaatgct
    11161 ttcggaattc aacggccatg tggattcagt tccttatatc actgccgcag aagacattct
    11221 tattgagcgc actggtggac gatttacatg ccgggccagt ggacatattt atcacgaaaa
    11281 gtttaatccg ccatccaaag ttgggatttg tgatgtagac gggtccgagt tatatcagcg
    11341 tgatgacgat aagaaggaaa ccgttacaaa gcgtatccgt gtttatcttg accaaaccat
    11401 gcccttggtt gagtactatc gtacaagagg caggttggtt gaaattgatg gtgatcagcc
    11461 ggtagagcag gtaacacaaa ggctcctggc tgctctgaaa aaataaattg ttgttattgt
    11521 gcagagcgac ttattagctc tgctgaaatg caggttttat tcaatgagtt gggaacgcca
    11581 gatcaaagta aaaagccccg cagagttgaa gatcatgcgc gaagctgggc atatcaatgc
    11641 atcagttctg gctgcagtaa gagaactttt acaacctggt gtgacgaccg ccgacctcaa
    11701 cgcggctgct gaggaagtgc tgaagtcaca cggatgtgta tcgccattca agaattatgg
    11761 tcatccgcca ttccccgcgt ccattaccgt ttcaatcaat gatgagatgg tacacgggat
    11821 ccctcatcca aaacgaaagt taaaggatgg ggatattgtt tcaattgatt gcggaacaac
    11881 ctacaatggc tttgtcgcag actccgcttt cactgccgga gtaggggaga tatctcctca
    11941 agcgacaaaa ctgctcgaaa tcaccgaaaa cgccttgtat gcaggtattg ataaaatgcg
    12001 caagggaaac cgtacaggtg atgtctcagc agcgatacag aaatacgtgg aagataacgg
    12061 ctttcacgtg acacgtgagt acacagggca tggtgtcgga agaaaaatgc atgaaggccc
    12121 tcaggtacca aatattggta tcccagggac cggtttgcaa ctccgcccgg gattgacaat
    12181 tgccctggaa cccatggtac tcgttggaac gcatgaaacc cgcgttctgc ccgatcaatg
    12241 gacggttgtc tctgctgatg ggtctttgac agcacacttt gaacatacgg ttgctgtcac
    12301 cgaaggagaa cccctcatcc tgactgtcct gtaaccttgc aggcaaccga aaaagtatgg
    12361 acggttcgta aaacaaccag tataattaag tctttggtcg tcagaaggcg accccaagta
    12421 aggagattga attcatgaaa gtatcaccct ccattcgcaa gcgttgtgcc aagtgccgca
    12481 tcattcggcg caaaggtatt gtttacatca tttgcgaaaa tccaaaacac aagcagagac
    12541 agggttagtg tggtgaccta tggcgagaat tgaaggtgta gatctacccc gcaacaagcg
    12601 ggttgaagtt ggactgacat acctttatgg tattggccca acccgcgccc aggcgatttt
    12661 ggctaacacc aaagtcaacc ccgatacgcg catcaaagat ttaagcgaag cggatgttgc
    12721 cgcgattcgc gactatattt ccaagcatta caaagttgaa ggcgatttgc gccgcgaagt
    12781 gcaaatgagc atcaagcgtt tgatcgaaat tggatcatac cgcggtttgc gccatcgccg
    12841 taacttgccg gtgcgcggcc agcgcacacg caccaatgca cgcattcgca aaggaaccaa
    12901 gaaaacggtt gccggtcgcg gtcgtcgcaa gggcgccaag aagtaattct gtccgaaagg
    12961 tataaataaa aatggctcag agtgttcgtt ccacccgccg cccatcaggc gctaaaaaag
    13021 ggaagcgtac cctgtcctac ggacaggtgc atatctttgc ttccttcaat aacacaattg
    13081 ttactgtaac cgacacagaa ggtaatactg tttcgtgggg ttccgccggt tccgctggct
    13141 tcaagggctc ccgcaagagc accccttttg ctgcgcgcct tgctgctgaa caggccttga
    13201 aagccgcgca acagcttggt ttgcaggatg ttgatctttt cgtaaaaggg cctggccccg
    13261 gccgcgagaa tgcgatccgc gccgtccagt ccatgggttt gaaagttcgt tctatttcgg
    13321 atatcacccc ggtgcctcat aacggctgcc gccctccgaa aaagcgacgc gtgtaattta
    13381 atgaggttgt taccatatgg ctaaaacatt aagtccagtt tgtaaacttt gcaggcgcga
    13441 aggcgaaaaa cttttcctca aaggtgagcg ttgctttacc cccaaatgtt cctttgaaaa
    13501 gcgtgatttt gcccccggcc agcatggacg taccgccggt cgcggcggtg gtaaaggaaa
    13561 agggatgggc actggacgcg cttcagactt tgcccgtcag ttgcgcgcca aacagaaagc
    13621 ccgtcgtatt tacggtgtga tggaacgtca gttccgccgc tactacgata ctgctaaatc
    13681 tggtcagagc ctcacaggtt taaccctgct tcagattttg gaatcccgcc ttgataatgt
    13741 ggtgttccgt cttggttttg cctcgagccg ttcacaggcc cgcctgctcg ttgctcatgg
    13801 tcatttcaaa gtaaatggcc gccgcaccga cgttccatcc atgcttctta gtgaaggtga
    13861 tgtagtcagt gtgcgcgaag gctccagcaa gttgacctat ttcaaggacc tgagcgccta
    13921 caccgaaaaa cgcactgctc ccagttggtt gaatcgcgat atcaaagcga tggctggtac
    13981 cttggctcgc atgccagaac gtgctgaaat tgacggaagt ctcgacgaac aattgatcgt
    14041 cgaatactat tccagacgct aatcttccat tttcgctcca ggctggacct gtaagctggt
    14101 acccagcctt gagccttata taaagggaaa ggtgaaccgt gactggtatc atcacgaaca
    14161 tggttatgcc aaaaattgag cgtgaagctg aagctcgaaa ctatggcaaa tttacaatcg
    14221 gcccgctgga gggtgggtat ggtgtgacgt tagggaatgc cctgcgccgc gtcctgcttt
    14281 cctctcttga aggcaccgcc attacatcca ttcgttttgc ggatgtattg catgagttta
    14341 gcgatgtccc tggcgtgcgc gaagacgtta tccagattat gttgcaagtc aaacagattc
    14401 gcatcaaaat ggacggcgtg gatagtgccc gtatgcacct cgaagtgcgt ggagaaggaa
    14461 ccgtaaccgc cgctgacatt gtcaccccgg cagaggttga gatcgtcaac ccagatacct
    14521 acctcttcac tgtggacggc gcaaagacca aacacgacat tgaatttgtt gtggaacgtg
    14581 gtcgcggtta ttccccggct aacgaacgcg ccggacacat gcccattggc gaacttcctg
    14641 tagatgcgat ttacagccct gtaaagcgtg tgaattggga agtttcctct gcccgtgtgg
    14701 gtcagagcac aaattacgat aaattgattt tggaaatctg gacagacgga accatttcgc
    14761 ccgagcgcgc cctcagctcc tccgcccgca ttctgattga ccacctgcgc tacattgccg
    14821 gtatcaacga agaaatgctt gcggtcgcag tggaacgcga atcctctggc agccgtttga
    14881 gcagcgaact ggccgaaacg ccagtggaag ccctcgatct ttctgtgcgt gtcttcaact
    14941 cattgaagcg cacaggcatc accaacgttg gtgacgttct cgaccttctt gaaaaaggcg
    15001 agaccgcggt catgtccatc cgcaacttcg gcgaaaagag tctcgacgaa cttcgccaga
    15061 agatgcagga aaagggcttt atgaaagacg acacatcctc ggagagttag aagatgagac
    15121 attcagtagc aggttatatt ttaggacgca ccaaaagctc acgtattgcc ttgaggcgca
    15181 acctgatcaa acagcttttt acacacgaac gtatcaagac caccgaagcc aaagccgcag
    15241 cagttcgcgg tgacgctgag cggctgatta cgcttgcccg caatagtgcc aatggtaccg
    15301 atgcggagaa gctcagcgcc cgccgcctcg ccatttccaa acttggtgac aaccaactca
    15361 tcaaacgtct gtttgatgag attgccccac gctttgcgac ccgcccaggc ggctacactc
    15421 gtgtgacaaa actcggaccc agacttggcg atgccgctga aatggtcgtc cttgaattgg
    15481 ttgaagaata aagaaacatc tggcgctcag ctaaagccac tcgctgaaag ctgatagcta
    15541 tatggcacgt tacaaagtga tccttgccta tgacggcacc ggttttaccg gaagtcaaag
    15601 acaggcaaac tcccgcacag tgcagggcga gttcgaaaaa gcgctccgca gactgggatg
    15661 gtctgataag tccatcgtta tggctggcag aacagatacg ggagtgcatg cgacaggaca
    15721 ggtggccgca tttgattttg attgggctca taccgaggaa aaactccttc gggcgctcaa
    15781 ttccgacctt ccttctgatc ttgtcataaa aagcctgatc acggccccgg ccgattttca
    15841 cccgcgcttt gacgccgcct cacgcgaata ccgttatcgg ttgttttgcg gaaccatccg
    15901 cgatccgtta cgggaaaagt atttctggcg ggtatggccc gctctcgacg aagatgccct
    15961 taagcaagcc gccgccatct tcctcggcac acatgatttc gccaccatgg gttcgcccac
    16021 aaccccaaaa gggacgacgg tacgcacggt gacgaaagcc gaatggaaga gaatgccgga
    16081 tgatgagtgg cagttcgaag tcagggcaga tgcgttttta tatcgcatgg taagacggct
    16141 ggtttttgtg caagtctctg tcgctcaggg aaaggccccc ctcgaaaaag tccgcaaggc
    16201 tctggacgag aaaggcaaac tgccttcagg actggctccc gcgcaaggtt tgacgctggt
    16261 tgaagtaacg tatggatcac atgactagat tatgaaacgg agtgacgaaa gtgcaacaga
    16321 aaacatttta tccgaaagcc gctgaaattc agcgcgactg ggttgtaatt gatgccgaag
    16381 ggcagacgct gggccgcctt gctgcgcgcg ttgcgcatgt tctgctcggc aagcacaaac
    16441 ccaccttcac acccggtgtg gaaatgggag actttgtcgt agtggtgaac accgaacgcg
    16501 tcagcgtaac cggcaccaag accagcagca aactggaaag caagatctac tacagccatt
    16561 ccggttaccc gggtggtttg aagagcatct ctctgcgcga ccagcttgcc acgcatcccg
    16621 accgcgccct gcgcgatgct gtgtggggca tgcttccgca caaccgcatg ggtcgtgccg
    16681 ttttgaagcg cctgaagatt tacgcgggtc ccaaccaccc gcatgctgtg caaaccgcga
    16741 aagccgcgaa ataaaagagg taagagaata tggctcagta ttacgaaggc attggtcgcc
    16801 gcaaggccag caccgcccgt gtgcgtgttt ccaccggcag cggaaaattc acagtgaacg
    16861 aaaaggaagg cgccgccttc ttcacccgca tgggcgatgt cgaagacatc atgcgcccgt
    16921 tcagtgcggt ggggcaggaa gctgtcaagt atgatgtgag cgccgtggta aacggtggcg
    16981 gcacctccgg acagaccgaa tctgttcgcc ttggcattgc ccgcgccctt cagctcatca
    17041 acgccgaatg gacagccgcc ctgcgcaaga agggcctgtt gacccgagac gcccgcgtga
    17101 aggaacgcaa gaaaccaggt ctcaagaaag cccgcaaggc accgacctac accaagcgct
    17161 aaacctgtgc tgtggtgtgt cacgtatcaa gaattacgtg gcttattcga tcaattaaaa
    17221 cgtaatgcgt gacggctaac gcctgtttcg cattacgttt tctttgttaa ccaaggaact
    17281 taaaatggac ttttcccttg tttctcaaac atttgagact ctccgcatcg caccaccctc
    17341 gaaactgact ctgctcgagg cgcatacgtt agccccggag tttgcgcatg tgccaccctt
    17401 tccacctgat attccagctt tactgacggg gatcgcctcc cgtgaacttg ccttacaggt
    17461 ccgcaccgtg ttacggaccg tttatcccca ggaacatgag gtctttgtcg ttgaagccgg
    17521 agtggccaga gaacatagcc tgagtgagct tgggactggt gaattttccg agcaaacctg
    17581 cctgtatctt ccttcattgg cggcaggtac gtcgttcgaa tcttttgctg agatcgtggc
    17641 gcatctgcgt gcgccggatg gctgtccatg ggataaggag caaacccacc aaacactccg
    17701 aaaacatctg ttggaagaag cctacgaaac cctgtctgcc atggacgaga acaattccga
    17761 cgagatgcgt gaggaatttg gcgacctgct tctgcagatt gtgctaaatt cacagatcgc
    17821 aaatgaggct ggcgaattta ccatgaatgg ggtgattaaa gggatttatg acaagatcgt
    17881 acgacgtcat ccgcatgtgt ttggagatgt caaactcgat ggcgtggatg gcgttttgca
    17941 gaactgggaa aaattgaagg agcaggaacg caaagaaaaa ggggaggatg gcggcaagaa
    18001 aaagggtctg ttggatggtg tctctgcggc attgcccgcc ttgacccagg cacaggaata
    18061 tcaagaccgg gccgctcgtg ttggttttga ctggcccgag atcgaagggg tgttggataa
    18121 gatccgcgaa gagatcgaag agatcaaaaa agcgcagaac ctcgatgaag tgacagccga
    18181 acttggcgac ctattctttg tccttgtgaa tcttgcccgc tggcgcaaag tggatgctga
    18241 atccgctctg cgcggaacca accagaaatt taagaagcgc tttgcatatg ttgaacaagg
    18301 cgcaaaacag cagggtagaa atctttccga catgactttg gaagagatgg acatcctttg
    18361 gaacgaagcc aaacgtgtgg gaatatagtt tccttatcag actgtataag cccgaattaa
    18421 ccgcttgcct gtagatgatt tgtaaggatt cacggcaggc aagcggtata aaatctctat
    18481 tatggaatct cagctgggaa caccttccat acctcaaaaa cccaaatggg atctgcccga
    18541 tgtttgggca aaaagcgggc attgtcctgc atgcggggct acgaatttaa agatcacaca
    18601 cttgccggat tacccggatt atctgtcttg cgctcattgt caggtttcgt ttgaagtgga
    18661 aaatgggggg cgccatatcc gtttgaagta cattcccgat caattcgaat acgccgatgc
    18721 catgcttcgt ggacgttggg tggcggcggc agatttaccc gcgattttgc gcaaacctca
    18781 acccgttgtg aaggaagcgg atgaacctca actgccaaag cagcccctct cagatgcaga
    18841 aatctggaag cgggcggttg gcatgtataa attgggtaac aagccgcgta tgatccaact
    18901 gacgttaatg cagtctggcg taccccagac gcaggcggat gccattcttg aaaaattaaa
    18961 gcgactggca gaagaagaca ctcgaaagat gaaccagaaa ttctggatac tggccggtgt
    19021 cgcgcttttg ttgatcttct ctcttgctgg tggatggctg tatgggagcg ggaatctcgc
    19081 cgtgttgatg ggagatgcga cccctgtgcc cacccaaagc ggtccttcag ccgtggacct
    19141 gttgctggat cttgttcctg atgaagccaa gcctgccatc atcgacctgc caaaaacatc
    19201 tgtggatgtc ggccgcggac cgggacaggc atcctgtccg ccgacatcgg ggatggcggc
    19261 agaattattc ggcggggatg ctgccatgtg gcagcaggcg ggcgcttttg cagcctggca
    19321 gatgatcgac ccgatcaact cagccacgat cactgtgcca accgatatga cagccgccta
    19381 tgtggataac aggacgagac aattcctttc ggtgcatggt ccggcaacga tctacaacgt
    19441 gaattttttg gtgatctcct gttgaggctg tatcattttg agggatgggg aatttcaacc
    19501 gccattagat gttcattgta ataaatacca ttgatgcgaa gcgccttggg ttcagtgccg
    19561 tagattttga atccacagcg ctcgtaggtg tgtatggcag agaggttgtc tgtaacgact
    19621 gccagtttga caatgactac atcatgttcg gctgcccaac caaggcaggc atgaatgagc
    19681 atttcggcaa ttcgcctgcc gcgccattca ggttttacgt agacactgat gatcgttgca
    19741 ctatggtgat tcttttttga gttgttgcga acgatcccag ccatgccgat cggctgtccg
    19801 tgatgttccg aaaaaaacaa ggcttgttca tctacttgaa atgtcaggcg cttttcccag
    19861 tagctttgcg gttggagcag ggtttcttca taatctgagc caaatgcgga ggggtgattc
    19921 ttcagggcct caaggcgcaa ttcacgaaag acttgcaggt ctgcggggtt tatgtggcgg
    19981 atggtgaaat cagtttcgct tgggggcatt aagtttgttt cccaggtgat cggctttagt
    20041 tgcaggtgaa tcttgccagt gcttcttcga atagcggacc agcggtttcg gcttgcgggg
    20101 tatatacctt ccatgcgaag acacggtcgc cacaggtcca ggcggcgatg gcaccgaagg
    20161 gcacttcgct gatggtggtg gtgatgggga catacatcac gttcatgccg cggatcagtt
    20221 tcacatcaaa ggttccgttt ttggtatcgg cctggtcatc gagcagggtt tcgagcagga
    20281 attgtggatc agatgtgccg ggggcctgca gccagataaa gttaatgacc atggtcggga
    20341 tctgataggc ggcgaggaat ccgttattgt aggcgctggg ggtgagttga ttgagtgcgg
    20401 ccatgtggtc ataaaatgcc atgttgttgg gatgcccaat gcagaattga tattcgcaaa
    20461 acacacctgc gagcgaaatg ggtgtggccg aggggaacgg ggtcggtggg gtgtagggag
    20521 tggcggttgg gattgcggcg agagtttcgg ccactgcctg ttgaatttgg atgttagggt
    20581 cgagggtggg gagagtcgtt ttcggggagc aggctgccat tagaagcagg atcaggcaga
    20641 ggtttgggaa aagaagcgct gtttttttca tcccaaaatt ataaccgccg catttgaaaa
    20701 acagccccgt ccatttctga acggggctgg gtgtttttag tgtccgctgc cgggggcggg
    20761 tgtgccatca aagatttcgg gattgggatc gccttcgacg atgaggaatc cggcgaggcc
    20821 gcgctgcaat cttgagaggg catggtctac gagaatgaag cgacccggga cttcaagttt
    20881 gaattcgacc atggttgctc caccgggcgg gacaagggtg gtttgtacgt cggtgagcgg
    20941 agcagaggtg atggaggctt gatcgaagac acggtcgaag atttcgccga tgacatggaa
    21001 ggaggaggtg aggttggggc cgccgacacc gaagaagatg cggatggttt caccaacgtt
    21061 ggctttgagc ggtttttggt cggtgagtgc gccgaccgcg ccgttgaaga cgacgtattc
    21121 ggggtcttcg ttcaacaact tggtgacatc ggtggtttgc attccgtggt cgccgtaggc
    21181 tccggtggtg tagatatctc cctgcatgac gtagaactcg cggtcaacag ggggaaggcc
    21241 ttcttcgggt tcaacaagga tgagaccata catgccgttg gcaatgtgat tggccaccat
    21301 cggtgtggcg cagtgataaa catacaaacc cgggttgatg gctttggcag tgaaagtggt
    21361 ttctttgccg ggttcggttt gggttacggc agcaccgccg ccaggacctg tgacagcatg
    21421 gaaatcaacg gagtgattca tggtgcttga gccgctgttc ttcaagtgga cttcgatggt
    21481 atctccaacc cgcacgcgga tgaaaggccc gggcactgca ccgttgaagg tccagtaggt
    21541 gaaggtggtt ccatcagcca gctggccttc gacttcgatg gtttcaagat caacacggac
    21601 ggtctgcggt tcacggactg cgagtggggc gggcaaatca gttgggtcac ggacaacatc
    21661 tgcaccagtg gttggggctt gtggagctgc tgcttcggtt gaagtgctgt acgcgccctc
    21721 tggtaatccg ctcagataat taacaatgga attgatttct tcttcggtga gagcgtcttt
    21781 caaagtggca ggcatgacat tgggcgcgca agcgccagtg gggcaggtgg gggccacaaa
    21841 gagattcggg tctgcaatgg attcgcggat gtattcttca gtggtggtgg ctgtgccggt
    21901 gtaggcatcg cttttcaaat gttcttcagc ggcggtgttg atatcggaaa gatttggtgc
    21961 gatcacgccg accgcgttgg ggattccatc aatggcatgg cagcttccgc aggcgccttt
    22021 ttggaaggcg gccaatacct gtgggtcacc gttgctttgt tcggctgcca cgggcatggg
    22081 ttgatcggtt tcactcacca tcaacttgcc gcgcattccc aattcagcgt ggttggctac
    22141 atcgtcataa tattccattt ctccgtgggt gtttggcacg gtgaatgtca cggaggcttc
    22201 ttcacctttt tctgtgagga cagcggtgct ggcctgcact tcggggaagg tgaggttgtg
    22261 ctgaccttcg ccgccattga tcaatgtgac ggtgatggtg ctgccgggac gggcgctcag
    22321 ggttgggttt tcaagtccgt tgatctcatc gctgacgcca agaaagacca gcctgccttc
    22381 acgcaggtcg gtggtcagca cgtattcctt atccacaggt tgaaaacttg tccctgtaca
    22441 ggcagagacc aaaataccaa gaaaaatggc tgcagcaaga aatttcagaa atgttttcat
    22501 tttttactcc ttattaaggg ttttgaacgt tggcttgttt cagttcttcc agtgcttttt
    22561 gaccatcaag ctggtaggtt ttgaccacat ctttccaggt acaaaaacgg gcaaaaccac
    22621 agcccacaca acctgtgcgc cagtttaaaa aaagcctggc ggccccgggg atggatctca
    22681 aaatgtcttc aacactggct tcagaaattt caaaactttc gttcatggtg acgatagaat
    22741 atcgcactcc cccaaacctg tctttgcgct ggcgcaaatt ggcgtaagta aaaaattacc
    22801 tcctactttt cgagtccgtc tgcaatgcgc accagatcgt gcggtttgat gatgcggatt
    22861 cgctcacggc cagtggtgat gatgccctgc cgttcccagt cactaaacag acggctgacg
    22921 gtgtacagcg tggttccagt catctctgcc acatcctgtc tggaaaagga taattcgatc
    22981 gccgcatctt tttcggacct gatgcctgcc tgccctgcca ggcggaccag cgcattggcc
    23041 actctctgct caacacgttc cgtggcgagt tcgcggtagc gtgcctgcat ttcctgaatg
    23101 taggtggtca tgagggtcat cagatcgaaa gacaactgcg gacgtgtttg gatcatttca
    23161 tgcagaaatt cacttttgat ggccaatgcc gtgctatttt ccagtgcctg cgcactcgct
    23221 gggtaggtgg cctccgcacg gaccgctccc aacgcaccaa acatttgcca gggataaatg
    23281 attcgcaaat tgacctgctg cccgttcaca gaattttgca ttaactttac ctgtccgctg
    23341 gtaaggatgt aaagataatg agcggggtcg ccatgaaaaa agaagaaacc actttcttcc
    23401 attgaacgtt ccacgctgtt ttgcaaaatg agtcgcaggt catcgtctgt tgcattctgg
    23461 aaaacgatca cctgtttgag gtcttttggc gaaagattca tatttccatt ttacacccca
    23521 ttctctttga tgcagcgcaa agagaaaaaa agcccttttg agtatattga tgttgaaaaa
    23581 ggacttctta tgaaaaaaat ctttgttgtt ttaggcgttg tattcatgat ggctgtactg
    23641 gccatttgga aatatcagca ggttgaactg tatcaattcc atgggtcggt ttttcgggaa
    23701 cccaaacttg ccgctgagat cgttttacgc tcggcaagtg gcccggtgcg tttggcggat
    23761 tatcgcggaa agataaccct gctttacttt ggttacactt cctgcccgga tgtatgcccc
    23821 accacgctgg caaacttgaa attggcactg gagaatctgg ctcccgcaga tgcttctcag
    23881 gtgcaggtta tttttgtctc tgtggaccca gaccgcgata cccccgaaag gctcggaaaa
    23941 tatgtccagt tgttcaattc aggctttgtc ggcgctacag ggactcgtca ggaaattgac
    24001 ctgatgaccg atgccttcga tgtgtattac aagatcaata attccgagtc aaaagagaat
    24061 tacagtgtgg atcattcggg ttttgttacc gtgatcgaca ggcatggcta tgctgtcatg
    24121 agttggcctc atggaaccgc tccccgcgat gtaagcgaag acctgcaata cttgatcgag
    24181 catgatatgc cagtcagtgc gcaaattttg gcaggaccaa catacacccc tgtagtgtgt
    24241 gccgttaccc ttgctcctgc acatattcaa ggcggggagt ggctgtatca acatcactgt
    24301 gcgcaatgtc atggcgctga tttgggcggc aacgcctcct ggcaggtgga attggcagac
    24361 ggcacccgcc tgcctccacc tttggatggc aatggaaagg cctggcaata ttctgaaacc
    24421 gagttgatcg agatcataaa aaacgggcgt aatctggata agccgttgtt tatgccagca
    24481 tttaaaacca cgcttgcaga ttgggagatc agttttattc tcacttatct caagagccat
    24541 tggagcatta atcaactgaa ctaccagcac ggctttatga ccctaacccc gcaggcaacc
    24601 ccatccactt tgccgaccaa tacagcactc ccataatagg aatattttct gttagaccac
    24661 acacggggaa attggttgcc gtgttggatt gcatggataa aaagtgacat tcctctcctt
    24721 gacagaacct ctgctgcaat ttatcatttg aacaccctac ccccctgggg ggggtggaaa
    24781 gcagaggtga tgatggaaaa cgacaacaca ctacgaagat taaagaccgt tgaaggccat
    24841 ttacgcggag ttatccgcat ggtggaggaa gatgcttatt gtatcgatgt catccgccag
    24901 attcaggcga tcgaagcggc tttgaacaag gttagctcga agatattgga agatcatctt
    24961 aactcatgtg tcattaccgc cattcagggc agtgacaaaa aggaacgtga gcgtgtgttg
    25021 aaagagatca ccgaagtgtt cgaaatgtcc accaaagttt aagagttgac aagttgctgg
    25081 cagccgcttt ttgcccaaac cctgcaaact tgaaacccgt aaatccgaaa caaataaaaa
    25141 ggagtaaata ttcatgacca cagttaccta taccgttccc gccatttcct gcggacattg
    25201 cacccgcacc atcgaaaccg aagttgctga cttgcagggt gttcaaaccg taaaggctga
    25261 gatcgaaagc aagaaagtga ccatcacttt cgatgccccc gcagatgaaa caaagatcaa
    25321 agctttgctg gcagagatca actaccctgc cgaaggcctg ctgacactgt aaacctcaga
    25381 cctgccgggc tttgaagcct gacaggtcat tttttttcaa ggaatttcaa tgaccgacac
    25441 caagcaactc acccttccca tcaccggcat gacttgcgcc aattgtgtgg caacagtcga
    25501 acgtaatttg aaaaagctgg atggtgtaca aagtgcggtg gttaatctct cctctgagcg
    25561 tgccactgtg gattttgatg ccgccaaact ggggctgacc gacgtcattg cgcgggttaa
    25621 tcgtgcaggt tatggcgttg ccacaggcga agcggatttg atcattaagc gcctttccga
    25681 tggcaacgat gccgcacggt tggaaaaagc cctcgtcaaa cttgaagggg tgctcgaagc
    25741 acaggtgacc tttgccaatg aaaaagcccg tgtgaaatac attcccacca tcattacgca
    25801 ggccgaattg cgccgcgcgg tttcatcggc aggttttgag gccctggaat tggggggtga
    25861 ggcggaagat gcggaagcgc tggctcgtga acacgagatc aaagaacagc gccgcctgct
    25921 tatcattggt ctggtgttca ccgttccact tttcctgctt tccatgagca aggattttgg
    25981 cttgctgccc gatttctttt acaccggcac caccatggat gggatgcgcg atgcccaacc
    26041 ctggttcggt tggttgatgc ttgtgcttgc actgcctgtt caattttatg tgggctggca
    26101 gtattatgtt ggggcgttca aagcgctgcg gaacagatct gccaatatgg atgtgctcat
    26161 tgtaatgggc tcttccgctg catttttcta ctccctgccc atcacctttg gctggctgat
    26221 ggggcacgtt tattacgaga ccgccgcggt catcatcacc ctgattaagc tcggcaaatt
    26281 tctagaagcc cgtgccaagg gacgcacctc tgaagcgatc aaaaaactga tgggactccg
    26341 cgccaagacc gcccgcgtca ttcgtgacgg ggtggaggct gaggtctcga ttgacgatgt
    26401 gcgtgtcgga gacatggttc tggtaaagcc tggcgaaaaa attcctgtgg atggcgcggt
    26461 tgtggaagga cgtagcgcag tggatgaatc catgttgaca ggtgaatcgc tgccagtgga
    26521 gaaaaagcca ggtgacgcgg tcatcggggc caccctcaac aagctgggca tgctcaaatt
    26581 tgaagccacc aaagtgggca gggaaaccgc ccttgcacag atcatcaaac tggtggagga
    26641 tgcccaaggc agcaaggctc ccattcagaa aatggccgat caggtttcag ccgtatttgt
    26701 tcccatcgtc atcggcattg ccttcctgac gtttttagcc tggtactttt tcgggccagc
    26761 cctgccgatc aactcagaca ttgataattt cacccgcgct ctcatttaca tggtggctgt
    26821 gttggtcatt gcttgcccgt gtgccatggg gcttgccacc ccaactgcca ttatggtggg
    26881 cacaggcaaa ggcgccgaag cagggattct gtttagaaac agcgaagccc ttgaacgggc
    26941 gggcagggtt tcggtggtgg tgctcgacaa aacgggaacg atcaccaaag gtcaacccgc
    27001 tgttacagat atagttacca ataacgaatc accaatgact ggtgatgaac ttctccgcct
    27061 cgctgcttcc gtagagaagg gctctgaaca cccgctgggc gaatccattt gggccgaagc
    27121 caccacccgt ggattggttt tagtggaacc cgctggtttt aaagcagaag ccgggcatgg
    27181 cgtgcaggcc gaggttgagg gaagaagcgt aatggttggg aacaggcgaa tgttcgaggc
    27241 gaggggaatc cagttgggga atctcgaatc cgaagtgaat cgtctgcaat ctgaagcaaa
    27301 gacagcaatg ttggttgcag tggatggcct tgccgtcggc atcattgccg ttgcagatac
    27361 cataaaagac acttcgaaag acgccattac caatttgcac gaaatgggtt tgaaggtggc
    27421 aatgatcaca ggtgacaacc aaaagaccgc cgatgccatt gcgaaacaag ttggcattga
    27481 catggtgctg gctgaagtgt tgcctgaagg taaatctgct gaagtaaaga aattgcaaag
    27541 tgaccaatct ccaaccaccc atgtggttgc catggtgggc gatggggtga acgatgctcc
    27601 tgcgctggcg caggcagatg tgggccttgc cattggcaca ggcaccgatg tggctatggc
    27661 ggccgcccct gtgaccttga tgagcggcga tttgcgcggc gtgtcacgtg ccattttgct
    27721 ctcacgcaaa accctttcca ccatcaagca gaatttgttt tgggcgttca tttacaacgt
    27781 tatcctcatc cctgccgccg cgcttggata tttgaacccg atgttggccg ctggcgcaat
    27841 ggcttttagc agcgtctttg ttgtgacgaa tagtttgcga ttgcgaagcg tcaagatata
    27901 gcccataggg cagtactctc ccgctttcta ttttctacag gaaaccatta acatgaaaaa
    27961 acttacactc cttcttctcg cagttctctt tctgactgca tgtgctccca aagctgccga
    28021 aatgcccaaa gctgaattac ctgctggcaa ggcctgggct gatggcaagg aaatttactt
    28081 catccatact gaagcctccg atgcaggtgt ggctgaaaaa ctcaccaaca tgatgaaatc
    28141 ccccgtggtg cttgtgcctt ctctggcaaa cgtgcccgcc gaatcgcttg ccaatgtgta
    28201 tgtcttcacc aatgggttgg caggcacagg ccccttcggc tttcaggctg atgtattcga
    28261 caacccgcca ggcactgacg gatatacccc tctgcgcagt ttgcatctga ttacatggac
    28321 cgatgaaacc aaggcccgtg aactcacttc cgctgccgat gtgctggccg ctgaagctgc
    28381 tggcgaattg accattgaac agcctggcgt tgtcattaac atgcctttcg tcgtctggga
    28441 tggcggcaag cgctaattcc actttccgcc ttttaccttt attgaggacg aatgaagaaa
    28501 aacaagaaca aaccctttga ccccgtcatt atcattaccg tgggagttac ctttctcgcc
    28561 gtcgctttca tgctgttggt caaaccccaa tcacttgcag gcgactcgag tgccatgcct
    28621 cagttgatgg tggccttcgt tacggggctt actacgggcg ggttgagttg ccttgcagta
    28681 cagggcggcc tgctggcgag ttcacttgca caccagatcg aacaggatta cgttgcgggg
    28741 caggctacaa aaaaagccgc ctcgccccgc accaatagcg ccctgccgat tctattgttt
    28801 ttgatttcaa agatcgcagc ctataccctg ctcggtgcgc ttcttggcat attgggttcc
    28861 taccttaccc tcagcccgct cacccgcgcc atgttgatga tcttgattgg catctttatg
    28921 atcggcaacg cgctgcgcat gttcaacgtc catcccatct ttcgatactt ttcgatcgag
    28981 ccgccgtcat ccatcaaacg ctacatccgc aaaaccgcca aaggcaccga tactgccacc
    29041 cccgtttttc tgggcgcact cacagtgctt atcccctgcg gggttacaca agccatgatg
    29101 gcaaccgccc ttggcacagg cagcgcagga atgggcgccg cgctcatgtt cgcattcact
    29161 cttggcacca gccctgtttt cttcatcgtg gcctacctca caaccgaact gggctcaaag
    29221 cttgaaaaat tcttcatgcg atttgtagcc gtggtggttt tgattttggg atttgtaacc
    29281 ctcgatggtg gactcaacct gatgggctcg cccctctcgt ttcaaaacct gacccgcaac
    29341 ctgcttccgt ccgagaacaa ttccaccacc caggctgtat cttccctacc cgccgccgaa
    29401 ggagagatcg tcctcgaagt acaaaacagc ggatatttcc ccaccaccct gaaagcccct
    29461 gcaggcaagg aacttaccct caacctcatc accgatcaaa cctattcctg cgcccgcgat
    29521 tttgtcatcc catcgctgga ttactatcaa ctcctcccag aaacaggcac gacccaggtc
    29581 aacattcccg cgcaagagca gggcagcacc ctcttcttta cctgctccat gggcatgtat
    29641 acagggcaga tcgtgtttga ataaaaatga aagagacctc caatgctcaa gaaaaccttc
    29701 aaagttgaag atatgacctg tgccaattgc gccatgaaac tggaggcgct tgaagatacc
    29761 ctcgaagggg tgaaggaaat caacgccagt tatcacagac tcgaaatggt catcgagtat
    29821 gacgaagcca aactgaccga cgaacagatc gtggcagccg taaagaaaaa gggatataca
    29881 gcagttgtaa aataggaaaa aaagagataa gaaaacaagc gcacaagtaa gaatcgacgt
    29941 gagcttgttt tcttttttca tccttcatcc ttcatccttc atccttcatc cttcatcctt
    30001 catccttcat cctttttctc cgtttccgtt atactcccca catgccaaaa tactataccc
    30061 tccatgaagc gaacaaaacc ttgaccatca tcgaacccat gttgcaggaa atgatggaga
    30121 tcgcaatgaa gatccgcgaa aatcagcctg agttatggga tctggtgcgg aaatctgctg
    30181 gcaatggcgg gaatccccac ttaagcaggc tgcttgtgga atttgaccac ctcgatgccc
    30241 tattccatca gctgcaggat atgggagttg tggtcaagga ccttatcatt gggctgatcg
    30301 atttcaccgc cctgcacaat ggcagggagg tctacctgtg ttggaaatat ggagaggggg
    30361 cgattcagtt ttggcacgag atcgaagcgg ggtttgctgg gcgtcaaaga attgattggg
    30421 aatagtaagt tgccaactcg ctcaaattga cctatactag cccctatcta tcttataaac
    30481 agcccgaggg aagatgaccg tatctacaat tggccgttac caagttgaga gtgaaattgc
    30541 acagggcgga atgggtgtca tatttttggc gcgtgacccg tatattcagc gccaggtcgt
    30601 cgtaaaagta ttgatgtata gccgtactct ggaggatgta tatcgggagt tcttccagca
    30661 ggaggcagag gtgatcgccg cccttgagca tccctgcatt gttcccattt atgattttgg
    30721 ctggcacggg gaacaaccat atatcgtgat gcggcatatg tctggaggca gccttgacga
    30781 ccacctgaga aaggccgagg tcaagctgac agacatggca cacatcctca agcgggtatc
    30841 agacgcgctg gatgcggctc atgccagaaa catcatccac cgtgatgtga aaccctccaa
    30901 tattctcttt gactccacgg gcgaagcctt cctttcagac tttggcatag ccaaatcgca
    30961 gaagatttca gatgaagatg gcgaagaata catggtcggc actcctgcat atatgagccc
    31021 cgaacaaata aaaggcggcc gcctggatgg tcgttctgat gtttatgcca tgggtgttgt
    31081 tttgtatcgt ttactcacgg ggcagcttcc tttttccagc aattccacca cgggtcttat
    31141 caatgcgcat gtcaacatgc ccgtccccga cgttcgcctg gtcaaggcga atattcctgc
    31201 ggtctggcag gaggtcatta gcaaggccat ggcaaaagac ccttcagatc gttatgccac
    31261 tgcgggcgat ttcgcccgtg atgtgaacga agtggtttca ggcaagtggt ttttgagaaa
    31321 gttgtaagag acaacatacc gcccccatca atgaccttgt tgccagaaga acaaggtcat
    31381 ttttttgttg aactcatcca tctgtgtaga cattgttgga aggaagccgc attctgctcc
    31441 tctactggca gaggacctgc aaggcttgcc accgatttaa gctaatttgt aagccaaagg
    31501 agaaaaatga aaaaaggaat actgtatggt atcggcgctt atctgctttg ggggttcttc
    31561 cccatctatt ggaaattttt gcatgtagta cctgctttgc aggtcatcgg gcatcgcatt
    31621 gcatggtcgt tcctggtgct gggtgtttac atccttttga cgggtcaatg gcgcgatttt
    31681 cgtaaagtgg cctccaatgc caaaaccatc ggcatatatg cgattgcggg cgtcctgctc
    31741 tccattaatt ggcttatcta tgtttggggt gtgaatgcgg gctatattgt tgagaccagc
    31801 ctggggtact ttatcaatcc gctcatcagt gttttgctgg gggttctttt tctgcgtgag
    31861 cgcttacgac cagcgcagtg gcttccagtt ttcattgcgg cggttggggt gttctatctc
    31921 acctttgtat atggccgcct gccgtggatc gcgctctcgc ttgcgttttc attcggcttt
    31981 tatgggttgg tgaaaaagct ggcgccgctt gggtctttgt atggcctcac ccttgaaaca
    32041 ggcattgttt tccctgcggc tctggtctat ttgatcattg cacaggcggg aggcagcggc
    32101 gcatttatgc acgacggcct gcaagtggac ctgtttttga tgggcgcggg gatcgttacc
    32161 acgattccgc tgttgatgtt tgcctcggcc gccaaggaga ttccgctttc aatggtggga
    32221 atccttcaat acattgcgcc tacccttcaa tttctcatcg gggtgttttt gtatcatgaa
    32281 ccgtttgacc agtcacattt gatcgggttt ggaatcgttt gggtggcact cgtcattttt
    32341 tgggtggaga attacattgc caaccgtaat cctgtggagc ccatcccaga actgggggag
    32401 gggtaggata ggaagaccgt tcttggtgtg ccgcaccttg tctttgggta aaggtgcggc
    32461 acactttcgg cgctgattca tcatcgaagc aggggcagga ttcaggttaa gaaagaggcg
    32521 cgttttttac gtcgaagatg tataatgact tcagtcaaag aggtgaatat ggcaaacaaa
    32581 aaaggtgtca ttggaatctt gactgggggc ggcgatgtgc cagggttgaa ccctgccatt
    32641 cgcgctgtga cgatccgcgc gctgcgagag gggtatcaag tggtggggat ccgccgcggc
    32701 tgggccgggc tggtggatat catccgcgat aagaacgcag acaatagcaa caattaccaa
    32761 tttttatctg aagagatcgt gaatcgtgct ggccgcacag gcggaacctt tttgcatact
    32821 tcgcgcacaa agcccagttc tgtaaaaaag gcgagtgtgc ccgagcattt gcagggacaa
    32881 tacgccgagg aaaaaaatga cctgacgccc gaggtgttga agaaccttgc ttttttgggc
    32941 gttgattatt tgattcccat cggcggtgac gataccctga gttatggcgt gcgactgtac
    33001 aaagagggtg tgaaggtgat cgccattccg aagaccatgg ataacgatgt gcctggcact
    33061 gattattgca tcggctttag cacctgcgtg acccgtacca tttcgttgac caacagcctg
    33121 cgcaccattg cagggtcgca tgagcggttt ttggtgttgg aggtgtttgg gcggtatgca
    33181 ggttttaccg ccatgctgcc gacgatggca ggggccgcga accgttgtgt gatccctgaa
    33241 tataaattta atattgagca tttgaccgag ttgatgagca aggaccgtga tgccaacccc
    33301 agccgctatt cggtgttgtt gatttcagaa ggcgcaatgt acgaaggcgg cgaaatggtc
    33361 tttgaagacc gcaccaccga tgcatatggc catgcaaagt tgggtggtat cggcgatttg
    33421 gtttcctcgc agatcaagga gatttcgccg aaatttaaca aaggcaagac cgtggacgtg
    33481 gtgaaccaga aactgggtta catggtgcgc ggcggtgacc ccgatgccat tgattcgatc
    33541 gtgccgatgg cgttcggcaa cctggcgctt gacctgttgt tgaagggtgt gcatggccgt
    33601 ttggtggtgt tgaaaaacgg acgttacgac aacatgccgt tggatgtggt gacggcgagc
    33661 aagaaggttg tggaagtgaa gcatttctac gacaccgatc ggtatcgtcc aatctatgaa
    33721 aactttgaaa tgaagccgct tttcattatg acgagcgaat aacggttttg gaaaacgatt
    33781 tgccccgtca ggttcacgtg gacctgacgg ttttattttc aaataaggga aacctttgct
    33841 gtgaagttct gtggatggga tttttctttc cgagtgtctt tgtggcgaaa aaggatctta
    33901 gactctttcc aaggtaacaa aaccgccaat tccaaagatg gattaaagac cgaattggtt
    33961 ataatt
//
LOCUS       DAOQJC010000035        33929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_567_length_33929_cov_8.540651, whole genome shotgun sequence.
ACCESSION   DAOQJC010000035 DAOQJC010000000
VERSION     DAOQJC010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 33929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33929
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_567_length_33929_cov_8.540651"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PLE39_07225"
     CDS             <1..527
                     /locus_tag="PLE39_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68154.1"
                     /translation="ASPSEVARGTFAGGLNQKSTRNGDSLDVRMKPAKEFVDFPFFGG
                     GNQLDWDVALNANLPMALTMNLGANKAALDLRDLTITDLKLETGASETKLTLPSRGRF
                     RADLDLGAASLEVIVPAGVSARIRATVGAVDLKVDESRFPRSGGYYQSQDYDTAANAV
                     DLSVDAGAASVKIS"
     gene            539..1306
                     /locus_tag="PLE39_07230"
     CDS             539..1306
                     /locus_tag="PLE39_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68155.1"
                     /translation="MKRFDPNIVFGFLLLVGGGLALAQTMGILENASRLFWGGLFLSV
                     GLVFLTLLFGGHWWAAIPGFTLLGLGALVLLPKSLDEIGGALFLGGIGLGFWYVFLSA
                     REERWWAIIPACVLSALAVMIVAAQRYEELGGAIFLGGIGLAFLFVFFTERNSRWWAL
                     IPAGVLVTLATVTVAAQRFGEFQTAGIFFFGLAITFLLVAMLTGMRWAYWPALGLGVM
                     GVLGIISLLELANYLWAVVMIAAGGFLLFRYFSNRNV"
     gene            complement(1665..1910)
                     /locus_tag="PLE39_07235"
     CDS             complement(1665..1910)
                     /locus_tag="PLE39_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNE68156.1"
                     /translation="MVATQIQTQEMQLRGLLEKLSAYVEQFHGGTVEFVSFDGKALRV
                     HLGGACLNCPLLPSTLHGWVAGTVHQFFPDIEVIEEK"
     gene            complement(1916..2383)
                     /locus_tag="PLE39_07240"
     CDS             complement(1916..2383)
                     /locus_tag="PLE39_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE68157.1"
                     /translation="MLELLPNIPLFENLDTAQIALLKPLFEQFNCAANTVVFEQGEPA
                     SYLYLLLKGEMLIRFKPYDAPPITLTRLRDGDVFGWSAVLGSTHYTSSLVSETQIESI
                     RIRGSHLLMLVRDHPETGKIIMDRLANVVSSRWKNARTQVQALLNSTNQNIKE"
     gene            2536..2736
                     /locus_tag="PLE39_07245"
     CDS             2536..2736
                     /locus_tag="PLE39_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68158.1"
                     /translation="MTEISELSALERLLLGLDAPPKLETEPLRKDALCPQCHLGRLDY
                     NGLLQLECPACGYVNGEGGGCT"
     gene            2785..3363
                     /locus_tag="PLE39_07250"
     CDS             2785..3363
                     /locus_tag="PLE39_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNE68159.1"
                     /translation="MTDSPLIESPQPPTARPFAAMLIGAGLIAIGIMFYMLLSQQDVS
                     SQDFSAVPVKVDYPAPKLDLVDLSGKSVSLDDYLGSVVLVNLWATWCPPCREEMPTLQ
                     TFYEKYKEDGFVLLGVDQEETAAVVQPFVEEFGLTFPVWLDLDYLAQREFNTMNLPSS
                     YVIDRSGTVRLMWIGGISKRNLEKYVPDLIAE"
     gene            3367..3834
                     /locus_tag="PLE39_07255"
     CDS             3367..3834
                     /locus_tag="PLE39_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNE68160.1"
                     /translation="MDVLLNWKGKMSFEGAGDSGFVQKMDSDSSVGGDNNGARPMEFI
                     ALGLAGCTAMDVISILQKKQQPVVDFKVQIHTERANEHPKVFTEAVIEYVVTGNGVDE
                     AAVLRAIELSAEKYCPAQAMLSKAFPIRMVYKILGDGGETIKEGEYFSKRDSV"
     gene            complement(3936..5420)
                     /locus_tag="PLE39_07260"
     CDS             complement(3936..5420)
                     /locus_tag="PLE39_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE68161.1"
                     /translation="MNNLSRIKQKLSSAFLVFAFLLSACNLEASVEPQVPTPTSDIAP
                     YSFAPTALPTRPTYKPGELVDYIAQNGDTLPALAARFNTTVEEIFQANPFIPREATTM
                     PPGMPMRIPIYFLALWSSPFQIIPDHAFVDGPTVIGFNTSAFVASQPGWLKDYRVYAG
                     GDWRSGAEMVDYVATNYSVNPRLLLAILEYQGGGLTAAEPPVKKNLLGFTRRYWENPY
                     LQLVIAANTLNNGYYGWRTGEMIEFEDTNEIIIRPDPWQNAASVSIQYYFSRIYDGQK
                     YAVATGPQGLFLTYEKFFGNPWQDSTIIIPGSLAQPEFQLPFPSDQTWYYTGGPHTGW
                     GTGEPLAGVDFAPPSDKSGCFVADDKNFAVAMADGLVVRSGVEGVLLDLDKDGNERTG
                     WVIFYLHLATKDRAPLGAELKTGDKIGYPSCEGGRATGSHVHIARKYNGEWIMADGIL
                     PFNLNGWITHNGTREYLGTLTKGSIIVTACECGDAFTAISGSFP"
     gene            complement(5413..7041)
                     /locus_tag="PLE39_07265"
     CDS             complement(5413..7041)
                     /locus_tag="PLE39_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE68162.1"
                     /translation="MSRTLAILIVLSLVLSACMSSSPSLWGVPQTPTPNTVDLASAPI
                     DPFAIQDHPIDFPTSTVPPEIATENAYTPTPTITPNSSTPVIEPTYTLAYDVAPFLYY
                     AQSGDMLSAVANRFGVDESEITSDADLTKTTLIDPGTLLVIPNRITEPMTPYVQIMPD
                     AELIFSYTAVDFDVKEYVQQSGGYLSKFKDYLGSTGWIDGDAAIERLATENSINPRLI
                     LAMLEFESRWVRGQPVDELHIDYPMGFNDYHYKGMSVQLTWAINNLAIGYYSWRAGTL
                     THLEFPDGTTMRIDPRLNAGTVAIQYLFSRSHSPSQWEQIINPSSGFPAMFNEMFGDP
                     WARADAVNPIFPSALNQPPLVLPFEPDVEWSMTGGPHNAWGQINPKIYGPKNSVFAAI
                     DFAPATDHGGCDPTPTWVTAAAPGLVVRSSNGAVMVDLDGDGYEQTGWNLLYMHVGAK
                     DRVAVGTWLEVNDRIGHASCEGGVSTGTHLHFARKYNGEWVTADGPIPFIMSGWRVVA
                     GEKAYEGKLVRGEEVVIADPVGQKWSNIFRKENE"
     gene            complement(7080..8702)
                     /locus_tag="PLE39_07270"
     CDS             complement(7080..8702)
                     /locus_tag="PLE39_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE68163.1"
                     /translation="MSVQKKSSFKFLFRSIILHLLAAILVSCAPATATPAAHPTYDPF
                     LPVSEGTFALSPNIPTVTITSTPVPPTATREPTPTRVPLTIILPTAVPTDQPIVTPTP
                     DAERVLPTPRQDEAQYVVQFGDTLGGIAFMYGISVEDLMLSNNLTDANMLDVGMTLNV
                     PAPNPSEGGTSFKVIPDSEMVYGPGSAYFNIQEFIESQNGYLEIYEQDVSGFILSGYE
                     IVWIVARNYSINPRLLLALLEYQSGWVTNPEPFNTAYPMGLADEGHYGLYRQLTWAAN
                     TLNQGYYLWKANAISTWVLNDGTVVPVDPTINAGTAAVQYFFAQLDDRPVWEQDVSGT
                     GLLLSYYVFFGNPFDFAIEPLVPAYVSQPTMQLPFSEGETWYFTGGPHGGWDYGSAWA
                     ALDFAPPGDNGGCGVSPLWVRAVADGLILRASNGALIQDLDNDGYEQTGWVILYMHVA
                     EEDRVEPGEYVYAGEPIGHASCEGGISNATHLHLARKYNGEWIAADGPLPFNLDGWIS
                     SGGGREYDGYLSRNGITIEALDSANELNQVYR"
     gene            complement(8811..9257)
                     /locus_tag="PLE39_07275"
     CDS             complement(8811..9257)
                     /locus_tag="PLE39_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE68164.1"
                     /translation="MTTVRKLLEAKETSKNYSIASTDTVLKAIRLMAELHIGALLVTD
                     GGKIVGIYTERDYLYKGENEGRAAKDTLLKDVMVSSMITVTKDTSVEQCMALMKQYNI
                     RHLPVVEHDQLVGLVSMRDVMFAALENKESEIRGLENYIMGSGFQS"
     gene            complement(9349..9831)
                     /locus_tag="PLE39_07280"
     CDS             complement(9349..9831)
                     /locus_tag="PLE39_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNE68165.1"
                     /translation="MKKTLIIGYGNPDREDDGVAWHILRALTLKLGLPAPASYEDEFP
                     QNGGIEFAFHLQLTPEMAEDIAAFEQVCFVDAHTGSIPEEVRLIEVESDFQRSPFTHH
                     LTPQSLVSICESLYQRKPEAVLLSVRGYQFLFSRQLSAQTEALVPQAVRLIWNWLQMS
                     "
     gene            complement(9903..10349)
                     /locus_tag="PLE39_07285"
     CDS             complement(9903..10349)
                     /locus_tag="PLE39_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE68166.1"
                     /translation="MTTVRKIVEEKAETKVHTIAADATVLDALQVMNEANTGAVLVTE
                     NEKIIGIFTERDYAREGEVKGKFAKDTIVRDVMTSQMVMIKLETTLQECAELMRQYRV
                     RHLPVLDNDRVVGMVSIRRLAEALLQEQKGTIVELENYILGTGYGE"
     gene            complement(10440..11867)
                     /locus_tag="PLE39_07290"
     CDS             complement(10440..11867)
                     /locus_tag="PLE39_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HNE68167.1"
                     /translation="MSTQKITIEPVTRIEGHAKVTIHMKADNTVDHAYMHVNEFRGFE
                     KFCEGRLYFEMPQITPRICGICPVSHHLAAAKAGDALTGQTPPRPANLLREMMHMGQM
                     IQSHGMHFFELAGPDLLLGFDASPEIRSVVGLIGANPELTVKAVQLRKFGQEIIKTLG
                     GRKLHPVFAVPGGVNKALTMAERDSILSGVDTAVATLQAGLQIMKDWAQKNMEDINKF
                     AVFPTGYFGLTTPENGLELYDGNIRLISREGVEMERFPVANYLDVIAEHVEPWSYLKF
                     PYYKKMGYPDGVYRVGPLGRLNLAEKIDTPLADRELKIFKSLNNGKPVENTLYYHYAR
                     LIEALFAAERVRVLCEDPDILSTDILNTRRNYTGHGVGVLEAPRGTLIHDYTADENGK
                     LLKVNLIVSTGHNNWAMTHAVDSVAKTYVKGPDVHEGMLNRVEAAIRAYDPCLSCSTH
                     AVGQMPIQLDIVAADGTLVKTLKRD"
     gene            complement(11933..12475)
                     /locus_tag="PLE39_07295"
     CDS             complement(11933..12475)
                     /locus_tag="PLE39_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HNE68168.1"
                     /translation="MNKPKIASDWMCGCAGCHMSLLDMDERILQIVELADLRATPITD
                     LKEPDESGVDVGVLEGGINNSANEEVAHKMRQRAKILVALGDCAVFGGVPAMRNLCGV
                     EAALRRAYVEAESNEPNSQIPNDPELATMTKARAIHEVVPVDYSVPGCPPDADTIFHV
                     LAELAQGRKPEIKDDLLHWH"
     gene            complement(12498..14477)
                     /locus_tag="PLE39_07300"
     CDS             complement(12498..14477)
                     /locus_tag="PLE39_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNE68169.1"
                     /translation="MAKITINEKQIEVPDGTTVLRAAEMAGIHIPRLCDHKELTPYGG
                     CRLCIVEVEGARVPMASCTLPVNNGMVVRTNTPALKQSREFILSMLFSERNHFCPFCQ
                     VSGGDCELQNSAYHEEMTHWPIQPGWSNFAVDTTHPYFILDNNRCILCRRCVRACGEL
                     AGNFTLSVAERGAASIIVADTDVPLGESTCIKCGSCVQVCPTGALIDRVSAYQGHDKH
                     LEEIKSVCSGCSVGCSIKIMVRDNRIVRIEGDWDGKVNRGVLCEHGRYDPVTESRKRI
                     TTPLVRKNGKLEPVSWDEAMGAVAGKLMENKNSIAAIASTRLSTETLSAFKELFSEKF
                     QSKLVTSVEEGVPTASVTRFAQTHEAFEGKLDVLRNADTVMCIGANLHRSHMVAGFMF
                     KRNLSKGTRLITIDPESSELDYMANIALKTQPGSDLALIRGLQAVIVQEELGRAPLSL
                     PDAGQLIEKAVSASGVSLEELRQTGRLLARSISPVILFGKGVTAQRDETIVEELHRLA
                     VLIGAVDSERHGLLSIKGEANSLAAALLSMDSAFELNGEKVVYAAIGDDYISKSLVER
                     VSKASHLVVQASYESKLTEQADVVLPVTIWAEQEGHYINLDGRIQESHKALVPPETVR
                     ENLAILNELAMRTNTTIKTDWQKAILARRSSVALN"
     gene            complement(14530..16170)
                     /gene="nuoF"
                     /locus_tag="PLE39_07305"
     CDS             complement(14530..16170)
                     /gene="nuoF"
                     /locus_tag="PLE39_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNE68170.1"
                     /translation="MIKKRAMILVSNDPESIRLGANEIVRHLNEALVTYGLQDEVDVS
                     TVSDVNHSNILPLVVVYPEAVVYGPVKAADVRYLVEEHLYKGRVVNDLLAPPKMLTGQ
                     IGWLRGHKGYNPAEQRIVLERAGRIDPESIEEYITANGYEALGKVLGEMTPEGVIDLV
                     EKSGLQGRGGAGFPTGRKWKFVRAAAGSKKYIVCNADESEPGTFKDRIVLEGDPHVIL
                     EAMAIAAYALGADEGYIYIRGEYGLAYRRLEKAILQAREFGFLGKNIFGTNFNFDIHI
                     HAGAGAYVCGEETALLESLEGKRGEPRSRPPYPVTRGLWNKPTSVNNVETLANVPAII
                     KNGAEWYRSIGTPSSPGTKVYTIMGNVNFSGVIEVPMGVTLREVINIYAKGMKPGSTL
                     KLAQTGGSSGSIIPAVLQDTPMDFESFRKAGVSLGSGALLICDQNTCIVDLVKVLLQF
                     FRFESCGKCTPCRVGTQRAYEIVDRISKGQGRLEELDTLLVLANEMERASNCGLGQTA
                     TVPIRDMLKHFRTEVEAHIRSGSCPTGVCEMAQEIEKV"
     gene            complement(16182..16679)
                     /gene="nuoE"
                     /locus_tag="PLE39_07310"
     CDS             complement(16182..16679)
                     /gene="nuoE"
                     /locus_tag="PLE39_07310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNE68171.1"
                     /translation="MTAPTIERDVVSVVQAAIEKHGNGRDAMIPVLSEINRQFGYVPR
                     EAIREVRKQLHDPARQVHIAESQLYTLTSFYDMLSTKPRGRHVIKFCENAPCHVVGGK
                     AIWDGLREKLNLENGETTPDNKWTLVTTSCLGLCSVGPVLLIDDDMYGNVTPEQITEI
                     LEKYE"
     gene            complement(16713..18497)
                     /locus_tag="PLE39_07315"
     CDS             complement(16713..18497)
                     /locus_tag="PLE39_07315"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNE68172.1"
                     /translation="MAFYRSHVLVCVDPECLAKGAHEVVDALQDEFVAQGLIDEIQVL
                     ETSRIGGCSDGPEMMVYPEGVHYHGMTADDIPFLVEEHFLKGRIVERFREVTKPVVDE
                     ELGPLRPKEVRVVLRNCGEIDPNNIEDYLATDGYQALGKVLFEMTPEQVIEEVSNSGL
                     RGRGGAGFPAGRKWGLTRQVQDNPKYMVCNADEGDPGAFMNRRVLEGDPHSVIEGMII
                     GAYAVGASYGYIYCRAEYPVAVETLNIAIKQAREFGFLGNNILGTDFNFDLEVRVGAG
                     AFVCGEETALMASIMGMRGEPRPRPPFPSVKGVWGKPSNNNNVETYSNVPQIILKGAA
                     WYASMGTERSKGTKTFAVGGNVVKPGLIEVPMGITLREIIFDVAGGIKDGKKYKAVQT
                     GGPMGGCLTEEHLDLPLDYEALTQAGSMMGSGGLVVMDETSCMVDIARFFMDFTQAES
                     CGKCTPCRVGTRRILELLQKICDGKGQDGDIEKLENLCHEVARNSLCGLGQGAPNPVL
                     STLKHFRHEYEAHIYEKRCPAKVCRPLIHYEIQAPTCTGCTVCARNCPVEAISGERRQ
                     AHHIDPDTCIRCGICMQVCNFNAIAVES"
     gene            complement(18548..18916)
                     /locus_tag="PLE39_07320"
     CDS             complement(18548..18916)
                     /locus_tag="PLE39_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNE68173.1"
                     /translation="MPAIKSLEELKRVREEALQKKKMKAAPGNVQVIVAMGTCGIAAG
                     ARDTMKSVLNYIETNNLSGITVTQTGCIGMCEQEPIVQVVIGDQPKVVYGKVNAEVAG
                     QIMKQHVQNGQPVKDYVLPS"
     gene            complement(18929..19498)
                     /locus_tag="PLE39_07325"
     CDS             complement(18929..19498)
                     /locus_tag="PLE39_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE68174.1"
                     /translation="MREIALHLMDIAENSVAAGGRNIRIAVHEDLQGDLLTATVSDDG
                     RGMDEETARQVMDPFYTTRTTRKVGLGIPLLKLAAEISGGRLSLVTEAGKGTKIEACF
                     RHSHIDRMPLGDLASTFLALLVSYPNIHWVFDYKTTDKNGNDDNFEFDDAELKNELGD
                     MSLTEPEILKFVRGLIEEGIQASALQTNF"
     gene            complement(19495..20007)
                     /locus_tag="PLE39_07330"
     CDS             complement(19495..20007)
                     /locus_tag="PLE39_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNE68175.1"
                     /translation="MTQSLKEIPATDPLSIAEQKQIIDTAITENKDRPGSVMLVLNEV
                     QGQIGHVSPSMQAYIARQLGVPLGQVHGVVTFYSFFKTKPRGKHTIKFCLGTACYVAG
                     VPQLIEKAKQMLNVELGETTPDGQITLEECRCVGACSQAPVVVVDEEVQGRLRPNKFP
                     QVLKKVQKPS"
     gene            complement(20124..20864)
                     /locus_tag="PLE39_07335"
     CDS             complement(20124..20864)
                     /locus_tag="PLE39_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNE68176.1"
                     /translation="MKNFRADLHVHTVLSPCAEVEMIPPLIVQEALERQIDIIAITDH
                     NASANVAAVQKAAHGTALHVFGGMEVQTREDVHLLCLFDHTEALANWQALVDSALPNL
                     KNNAEFLGEQFIVDEEGNFIRSEPRMLLTSTHFSIDEIFNKVNALGGLVIPAHVDRNS
                     YGLFPTLGLLSPNWPILSLEISRHISPEKAASLYPFIKNTPLIQSGDVHRLDEFLGKT
                     IFTIDQPSLAEIRMAFRNESQRTVNIEP"
     gene            complement(20877..21218)
                     /locus_tag="PLE39_07340"
     CDS             complement(20877..21218)
                     /locus_tag="PLE39_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68177.1"
                     /translation="MNLQQIIERLNLNLLTDPRDFETIHPEGGYTSDLLSCVMAGAKS
                     KYIWVTLQAHLNIVAVAALLDVAAIIITEDAQPDAATITKANEQGVILLSTSMPSFEV
                     DGKLWEIGLRN"
     gene            complement(21225..22232)
                     /locus_tag="PLE39_07345"
     CDS             complement(21225..22232)
                     /locus_tag="PLE39_07345"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE68178.1"
                     /translation="MCPNRIRTEQEIHGRHGRPITDQDAHEINRVEELSYDLKIHEVM
                     SKNLQTASPDISLSDVLKILRINRISGVPVVQDEKLVGVISIEDIVRALQNNDLTETV
                     GQYMTRELVTVASFDSIVKAMQTFAEKHVGRLPVVDEEKKLVGMITKGDITRGILVAL
                     QKDYKAEEVRRYRASHLFEDIISDRTTLVLRYNIKAQDFTHGGNASSHIKRALLRLGA
                     DAQIARRCGIAVYEAEMNLIIHTTHGGILKLEVEPHRITMSTTDDGPGIPDVEQVLQP
                     GYSTATDQVREMGFGAGMGLVNIKRCVDNMELESTVGKGTKLIMRIHVPAENFNARKE
                     G"
     gene            complement(22222..22578)
                     /locus_tag="PLE39_07350"
     CDS             complement(22222..22578)
                     /locus_tag="PLE39_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68179.1"
                     /translation="MNLNQVIELVGGTTLTTEADLNINIQGGYGADLMSDVLAASQPN
                     AVLLTGLTNPQVVRTAQMADFRAIIFVRGKQPQAETLELAEQENMPLITSPLGMFELC
                     GRLHKAGLPSFESNVS"
     gene            complement(23163..23936)
                     /gene="fdhD"
                     /locus_tag="PLE39_07355"
     CDS             complement(23163..23936)
                     /gene="fdhD"
                     /locus_tag="PLE39_07355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNE68180.1"
                     /translation="MNAPQKSIHYEQYEFQKWKSFDAETIVEMPVSLTVNGKVWISFM
                     CTPVNLEALAVGFLFNEGIIQEMDEVEDVRVCEHGDNVDVWLSHAAEQPTSWRRTSGC
                     TGGVTAVDLLAKPNVSLSGNRFTVRPDAIGQLVEMLFESQSLYRETGGVHTSALSDGE
                     KILIAAEDIGRHNTLDKIAGLCLMNDIWPENRILITTGRISSEMLQKAAQINAPILIS
                     RTSPSSLSIEMAERYGITLIGYARKHRFNVYTNGERVGL"
     gene            complement(23955..24602)
                     /locus_tag="PLE39_07360"
     CDS             complement(23955..24602)
                     /locus_tag="PLE39_07360"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HNE68181.1"
                     /translation="MPNLNLWKAGDHILLRGVYNGSPTYMQSARVVQDTPEETALLIW
                     PGAECMVPSGYLHHAHDRSMWDRWAETTTNTLKLEKYIWHTNRFLILLEPEKYFSTIY
                     IWEAAKDEFVGYYINFQLPYRRSHLGFDTLDLDLDIVIEPDFQWSWKDMDEYQQGIEA
                     GGIHADWVNEIERAKQEVFTRLEKRGYPFNSDWLNWRPNPNWTAPSLPENWDVID"
     gene            complement(24589..24804)
                     /locus_tag="PLE39_07365"
     CDS             complement(24589..24804)
                     /locus_tag="PLE39_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68182.1"
                     /translation="MDKEKAKNSKKFVALCGRIFRDADPKYFPSAQYRGRTLRFCTES
                     CLGAFLADPDIFYKVHRNSEKNKDAKP"
     gene            complement(24797..26518)
                     /locus_tag="PLE39_07370"
     CDS             complement(24797..26518)
                     /locus_tag="PLE39_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNE68183.1"
                     /translation="MTAHKWWQTAVFYQIYPRSFADGNGDGIGDFKGATEKLDYLASL
                     GVDAVWLSPHFPSPNWDCGYDISDYCNVAPEYGTLDDFKTFLNEAHKRNIRVILDLVL
                     NHTSDEHAWFLESKSSRDNPKADWYVWVDTPPNNWQSCFDGEAWTYVPERGQYYYHYF
                     MKQQPDLNWHNPEVKKAMWDAARFWLDMGVDGYRLDAIGTIFEDPQLTPHNVPMNLAE
                     LRRFSELANTPKEKKLKDKYWYEMFKHQYGKPGVHELMKELRAILEEYEGDRMLVGED
                     DDIAYMGNGRDELQLIFNFPLMRTDQITPAHIRKNQKDRLAALEKLPVETGWPCNTLG
                     NHDCSRIHTRYGDKIHDAELARLNAALVLTLKGTAFLYNGEEIGMTDFMLSDISHVKD
                     TMGSWYYHTIVTEMGVHPEEAMLRTAEMTRDKNRTPMQWSNSPNAGFSPSGVNTWLPV
                     NPNYKDGINVYDQDKNPSSLLHYYKHLLRVRKGSPALQHGEYLPLHNTSREYFAFLRR
                     SENQTVLVVLNYSDKVFELDFSRTKEIKDSNLRILFTSAERRGGSFAPRFKVGAFEVF
                     IAETERG"
     gene            complement(26593..26844)
                     /locus_tag="PLE39_07375"
     CDS             complement(26593..26844)
                     /locus_tag="PLE39_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HNE68184.1"
                     /translation="MNIPDRIISILELRRNTGERYSRMWIAHNGIVYDVTDCPKWRTG
                     MHEFLHFPGQDLSGELTDAPHEEEVFKHDCVKIVGRLES"
     gene            complement(26884..27921)
                     /locus_tag="PLE39_07380"
     CDS             complement(26884..27921)
                     /locus_tag="PLE39_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNE68185.1"
                     /translation="MKAIRFIGANQPLQMQEIPIPEIGERDILVRVKAAGICHSDAHY
                     RAGISPVKPVPLTLGHEVAGVVEKVGAQVGNLKIGERVCLHYNISCGDCYHCSTGNDQ
                     FCEKVLMLGHYTNGGYAEYISVPARNAIRLPDEIPFEQGATLMCASATAFHALRKSRL
                     KAGETVAIFGAGGLGQSAIQLAKAFGAVEVYAVDINDDKLNLAAQYGAIPVNGNKVNA
                     VEEIKRLTHGKGVDVAIEMIGLPLTMKQALQCAGVMARVVIVGLSNKPLEIQTYTELL
                     GNEVELIGSNDHLLQELPLLVDLARRKVLDTSKIVTRTVPLDANAINATLDSLEKFSG
                     DVRTVIVPASN"
     gene            complement(28005..28559)
                     /locus_tag="PLE39_07385"
     CDS             complement(28005..28559)
                     /locus_tag="PLE39_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNE68186.1"
                     /translation="MTPNHLTKEELEAGLQHIAQSPKDNGVVEMIVRRPEVDGREILE
                     SAELNLTEGLVGDNWKTRGSTATPDGSAHPEAQINIMNSRAVALLAQEKDRWALAGDQ
                     FFIDLDLGSENLPAGTRLALGSAILEVTAKPHTGCDKFAARYGRAAALFVNSTEGKKL
                     HLRGINAKVVQAGTVRVGDAVKKI"
     gene            complement(28581..29156)
                     /locus_tag="PLE39_07390"
     CDS             complement(28581..29156)
                     /locus_tag="PLE39_07390"
                     /inference="COORDINATES: protein motif:HMM:NF020581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNE68187.1"
                     /translation="MITARHKVLAHLKKTRAASAREIARALKMSAPNVRHHLSVLCSD
                     GRVESTEVNNREGRGRPEKLYSLSQAALGDNLSALTKALLTESGPRLNIEALANHILN
                     SGQFANLPITKRLVLLIEKINEMHYQARWEASAEGPRVILGRCPYAKIIAGHPELCKM
                     DAAILKNALGNSVEQTSKMENLHGVCIFAMR"
     gene            29333..29542
                     /gene="iscX"
                     /locus_tag="PLE39_07395"
     CDS             29333..29542
                     /gene="iscX"
                     /locus_tag="PLE39_07395"
                     /inference="COORDINATES: protein motif:HMM:NF016284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HNE68188.1"
                     /translation="MTLNWESTYAIALELRRQHPEVNIEDVSLQQIYQWTIQLSEFED
                     DLALVNDDILYAIYQDWFEENIHGK"
     gene            29532..30020
                     /locus_tag="PLE39_07400"
                     /pseudo
     CDS             29532..30020
                     /locus_tag="PLE39_07400"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            30170..31915
                     /locus_tag="PLE39_07405"
     CDS             30170..31915
                     /locus_tag="PLE39_07405"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNE68189.1"
                     /translation="MATAIQTSTVDLVARFPGKVTADTRPGYSGFIVNKDNLVEVATA
                     IRDEFGFDLLTAATGADYIAENKMEMVYHAYKTTGGPGLVFKVQVERVDPVEIPSLIN
                     IWPGVDFQEREAWDLLGIKFTGHPDLRRILMWEGYEGHPLRKDWKEPFFEEEAKPFKS
                     RWPDGQHTFAENKNPFRDNLKFPADFDPEKFEIVNEDGLYASLERHTMKNEEGSLNTD
                     HIVVNMGPHHPSTHGVLRVAVTLDGETIIGLKPVMGYLHRNHDKIGERNTYLQVIPYT
                     DRLDYFNSMSNNFGYVTTIEKLMKIQVAERAEYIRVIMAELSRVQNHLIFIGMLMNDL
                     GTMYTPSLYAFEERELVLDIFEAVSGARMMCNYFRFGGVVRDIPDYVMQKIKDLVFER
                     LPAKTDEMERFLNENEILVARLKDIHVLNAEDAIKYSVTGPVLRAAGVPYDLRRADPY
                     SIYDRFDFDVAVRQNGDMMDNYLIRFDEIRQSLRILEQALKQIPQGPINSQKPQYQVR
                     VPAGEAYGRIESPKGELGFYVVSNGKPNPYRYHVRPASFVNLQAVESMCIGTKIADFV
                     ALLAMLDIVMGELDR"
     gene            31930..32544
                     /locus_tag="PLE39_07410"
     CDS             31930..32544
                     /locus_tag="PLE39_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNE68190.1"
                     /translation="MYGKGILKGLSVTWKRFWDTYTEDISWMLRGKKRYNTVEGVAHR
                     SSKDTKGIFTVQYPEEQLIQPEEFRYVPFLVYDEGADGKKEVRCTSCGICSKVCPPQC
                     IWIVRTNDPATGRPVPVPTNFYIDMDICMNCGFCAEYCPFDAIKMDHDFDIASYDRNV
                     YNLEKLLKPAKYYAEIRPENYTREEEARKAKEAAKAAKAEAASA"
     gene            32630..33703
                     /locus_tag="PLE39_07415"
     CDS             32630..33703
                     /locus_tag="PLE39_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNE68191.1"
                     /translation="MTLSKESVLAALGKVQEPELHQDLVTLNMIRNLEISGDKVSFTV
                     MLTTPACPLRGRIEKEVREAVMAVDGVKTIDLKMDSDVPNDGRMRGLVSMPIRNAIAI
                     GSGKGGVGKSTVSVNIAVALAQSGARVGLMDADIYGPNTPTMLGVDKLPPPSGQKLIP
                     AEAYGIKMISMGLLVKPGQPLIWRGPMLNSAIRQFLGDVVWGELDYLIIDLPPGTGDA
                     ALSLAQALPLSGSVVVTLPQLVSLEDASRGLNMFKQLEVPILGVVENMSYLDLPDGTR
                     MDLFGSGGGEELAKATDTTFLGKIPIDQNVRIGGDTGKPIVAAHPESAVAKSIQEIAQ
                     KIAAKASVAAMSANNALPINIVE"
BASE COUNT         7535 a         8437 c         9126 g         8831 t
ORIGIN      
        1 gcgcatcgcc cagcgaggtg gctcgcggca catttgcagg tgggctgaat caaaagtcca
       61 cgcgcaatgg tgatagcctc gatgtgcgca tgaagcctgc aaaagaattt gtggattttc
      121 catttttcgg cggaggcaac cagcttgatt gggatgtcgc cctgaatgcg aaccttccca
      181 tggctctgac catgaatctc ggtgcgaaca aagccgcgct cgatttgcgc gacctgacca
      241 tcactgactt aaaactggaa actggtgcca gcgaaacgaa attgaccctg ccatcacgcg
      301 gccgcttccg tgcggacctc gatcttggtg cggcttccct tgaagtgatc gtccccgcag
      361 gagtctccgc ccgaatccgc gccacagttg gtgctgttga tttgaaagtg gatgagtctc
      421 gttttccgcg cagcggtggt tattatcaat cgcaggatta cgacactgcc gccaacgccg
      481 ttgacctgag cgtggatgcg ggtgcggctt cagtgaaaat cagttagtga ggtgtgtaat
      541 gaaacggttt gacccaaata tcgtgttcgg ttttctgctt ctggtgggtg gcggtttggc
      601 gctcgcacag acaatgggca tccttgagaa cgcctcaaga ttgttttggg gtggactatt
      661 cctttccgtt ggtctggtct ttctcacttt attgtttggc gggcattggt gggcggccat
      721 tccaggcttt acattgctgg gccttggcgc tttggtcctt ttgcccaaat cgcttgatga
      781 aatcggcggg gcgctctttc ttggcggtat cggtctgggg ttttggtatg tgttcctttc
      841 tgcccgtgaa gaaagatggt gggcgatcat tcccgcctgt gtcttgtctg cgctggctgt
      901 gatgatcgtt gctgctcaac gctatgaaga attgggtggg gcgatctttt taggcggcat
      961 cggccttgcg ttcttattcg tcttcttcac cgaacgcaat tcgcgctggt gggcgctcat
     1021 ccctgcaggg gtgctggtca cactggcaac ggtcaccgtt gcggcgcaac gctttggcga
     1081 atttcaaacg gctggcattt tcttcttcgg gcttgccatt accttccttt tggtagccat
     1141 gctgacgggg atgcgctggg catactggcc cgcactcggt ttgggtgtga tgggcgtgtt
     1201 ggggatcatt tcactcttgg aactggccaa ctacctttgg gcagtggtca tgatcgcagc
     1261 aggcggattt ttactgttcc gctatttctc gaatcgcaac gtttaaggtg agtaaaacag
     1321 ttgaacaaaa catgattcac tccaaactta gggaatcagc cgcgaattta tgtgaaagtc
     1381 tgaaagtcta accacggaga cactgagaca cagagaaaac aattgaaaac tccgtggctc
     1441 cgtggttcaa aatggccttt caaagacatg acacgagagg tcaaatgaat tgcaggatac
     1501 ggacgagata taaaaaagcc gacccaataa aatggatcgg cttttttcgt gtaaatccgc
     1561 ggctaacgaa ttgaaagatt atattcggga tgtttcatgt ttagattcac attctatgtt
     1621 gcgatggaat ctgaaattgc gcccagaata accgcttgag tgaattactt ttcttcaatg
     1681 acctcgatgt cagggaagaa ctgatggacg gtcccagcca cccagccgtg cagggtggag
     1741 ggcagcaggg gacaattcaa acatgcacct cccaaatgaa cccgcaaagc cttgccgtcg
     1801 aaggagacga actctacagt gccgccgtgg aattgttcca cgtaggcgct gagtttttca
     1861 agcaaaccgc gcaattgcat ttcctgtgtc tggatctgtg ttgcgaccat ggcttttact
     1921 cctttatatt ttgattggtt gaattcaaaa gggcctggac ctgggtacgc gcgttcttcc
     1981 aacgtgatga tactacattt gccagacgat ccatgatgat ctttccagtt tcaggatggt
     2041 cacgcaccag catcagcaaa tgtgagccgc ggatgcgaat ggattcgatc tgtgtttcac
     2101 tgacaaggct tgaagtgtaa tgggtgctgc ccagcactgc cgaccagccg aagacatctc
     2161 catcgcgcag gcgtgtcaat gtgatcgggg gggcgtcata aggcttaaag cgaatgagca
     2221 tttcgccttt gagtaacaga tacaaataac tggcaggttc accctgttca aataccacgg
     2281 tgtttgctgc acaattaaat tgttcgaaca gcggcttgag cagggcaatt tgggctgtgt
     2341 cgagattttc aaacaaggga atattcggca ggagttcaag catttataga atgttaatca
     2401 atgtccaatc aacaatccag tatcaaatat cataaacttg atgacatttt tccttttttc
     2461 aaaaaatgct ggtgtccctg ggttatactt tgtgaaataa gtaattttct gaatcttcac
     2521 ctcttcaatg aagtgatgac agaaataagt gaactctccg cgcttgaacg cctcctgctt
     2581 gggcttgatg cacccccaaa gcttgaaacc gaaccgttac gtaaagatgc gctttgccca
     2641 caatgtcacc tcggcaggct tgattacaat ggcttgttgc aattggaatg ccctgcctgt
     2701 ggttatgtta atggtgaagg cggcggatgt acctgatgaa tctctttttg ctccgtgtga
     2761 gcaaatctta ttggatgtga aaacatgact gactcccctc ttatcgaatc accccagccg
     2821 cccaccgcgc ggccctttgc tgccatgctg atcggcgctg gactgatcgc gatcggcatc
     2881 atgttctaca tgctgttaag tcaacaagac gtttcttctc aggacttttc agcggttccc
     2941 gttaaagtag attatcccgc cccgaaattg gatcttgtag acctttctgg caaaagcgtc
     3001 tcgcttgatg attatttggg aagtgttgtc cttgtcaact tgtgggctac ctggtgcccg
     3061 ccttgcaggg aggaaatgcc caccctgcaa actttctacg aaaaatacaa ggaggatggt
     3121 tttgtccttc tcggtgtgga tcaggaagag actgccgcgg ttgtgcagcc atttgttgaa
     3181 gagttcggcc tcacctttcc cgtctggctg gatctggatt atctcgccca gcgtgaattt
     3241 aataccatga acctgccaag ttcttatgta attgaccgtt ccggtaccgt ccgcttgatg
     3301 tggatcggcg gcatctcaaa acgaaatctt gaaaaatatg tacctgattt gatcgcggag
     3361 tgaaaaatgg atgtattgtt gaattggaag gggaaaatgt cttttgaagg ggcgggcgat
     3421 tctgggtttg tgcaaaaaat ggactcggat tcgtcggttg gtggtgacaa caacggcgct
     3481 cgtccgatgg aattcattgc attggggttg gctggctgta ccgccatgga tgtgatttcc
     3541 attttgcaaa aaaagcagca gcctgttgtg gattttaaag ttcagattca tacagaaagg
     3601 gcaaacgaac atcccaaagt gtttaccgaa gcggtgatcg aatacgtggt cacaggcaat
     3661 ggggtggatg aggcggctgt gttacgcgcc attgagcttt cagccgagaa gtattgccct
     3721 gcccaagcca tgctttccaa ggcgttcccc attcgtatgg tatataaaat tttgggagat
     3781 ggcggcgaaa ccataaaaga aggcgagtat ttttcgaagc gcgattcggt ttgattaaat
     3841 tggaaaaatg aatacggctc acctgtgaat gtcaaatttg caggtgagcc gtatggtttg
     3901 tgggtggaat tttttattga atttccctta tctatttatg gaaaactgcc gctgatggct
     3961 gtgaaggcat cgccgcattc gcaggcggtg acgatgatcg agcctttggt gagggttccc
     4021 aaatattcgc gggtgccatt gtgagtgatc caaccgttca agttgaatgg caggatgcca
     4081 tcggccatga tccattcgcc gttgtatttg cgggcaatgt ggacgtgtga ccccgtggcg
     4141 cgcccgcctt cacatgaggg atagccgatc ttatctcctg tttttaattc agcgccgagc
     4201 ggagcgcgat cttttgtggc aagatgcagg tagaaaatga cccagcctgt acgctcattg
     4261 ccatccttgt cgaggtcaag aagcacccct tccacgccgg agcggacgac gaggccatct
     4321 gccatggcaa cggcaaagtt tttatcatct gcgacaaagc agcccgattt atcggagggt
     4381 ggggcaaaat ccacgcctgc cagcggctct cctgttcccc agcccgtatg cggaccgccc
     4441 gtgtaatacc aggtctggtc actggggaag gggagttgaa attctggctg ggctaaactg
     4501 cccgggatga tgatggtgga gtcctgccac gggttgccga aaaatttctc gtatgtcagg
     4561 aacaggccct gaggtccagt ggcgaccgca tatttttgtc cgtcatatat ccttgaaaaa
     4621 taatattgga tcgagacaga ggcggcattt tgccacgggt cggggcggat gatgatttca
     4681 ttcgtatctt cgaactcgat catttcgccg gtacgccagc cgtagtatcc gttgttcagc
     4741 gtgttggctg caatgacaag ctgcaggtat gggttttccc aatagcggcg cgtgaagccg
     4801 agcaaattct ttttcacagg cggttcggcg gcagtgagac cgccgccctg atattctaga
     4861 atggccagca aaagacgcgg attgacactg taattggtgg ctacgtaatc aaccatttcg
     4921 gcgccgctgc gccagtcacc gcccgcatac acgcggtaat ctttgagcca gcccggttga
     4981 gatgccacaa aagcggatgt gttgaacccg atgacggtag gaccatctac aaaggcatgg
     5041 tctgggatga tctgaaatgg gctggaccac aacgccagaa aataaatggg aattctcatg
     5101 ggcatgcccg gcggcatggt ggtggcttcg cgggggatga acgggttggc ttgaaagatc
     5161 tcttcaactg tggtgttgaa gcgtgcagcc agagcgggca gcgtatcccc gttctgggca
     5221 atgtaatcca ccagttcacc gggtttgtac gttgggcggg tggggagagc tgtcggcgca
     5281 aagctgtagg gtgcgatgtc agatgtgggt gttggaacct ggggttctac agaggcttcg
     5341 agattgcagg ctgaaagtaa aaatgcgaaa accaggaacg cgctggacag tttttgtttt
     5401 attcttgaaa gattattcat tctcttttcg gaagatgttc gaccattttt gtccgaccgg
     5461 gtcggcgatg accacttctt cgccgcggac aagtttgcct tcgtaggctt tttcgcctgc
     5521 cacaacgcgc catccgctca tgatgaacgg aatcgggcca tcggcggtaa cccattcgcc
     5581 gttgtatttt cttgcaaagt gcaggtgggt gcctgtggat acgccgccct cacaagaggc
     5641 atgcccgatg cggtcgttca cttcgagcca ggtgcccact gcaacgcggt cctttgcccc
     5701 gacatgcatg taaagcaggt tccaaccggt ctgctcgtac ccatcgccat cgaggtcaac
     5761 catcacggct ccattgcttg aacgcacaac gaggcccggc gccgccgcag tgacccaggt
     5821 gggggtggga tcgcacccgc cgtggtctgt tgcgggagcg aaatcgatcg cggcaaacac
     5881 actgtttttt ggtccgtaaa tcttggggtt gatctgcccc caggcattgt gcggaccgcc
     5941 cgtcatgctc cattccacat caggctcaaa gggcagaacc aatggcggct ggtttaatgc
     6001 agaggggaaa atggggttca ctgcatcggc gcgtgcccat gggtcgccga acatttcatt
     6061 aaacatggcg ggaaagcctg atgacggatt gatgatctgt tcccactgcg aaggactgtg
     6121 gctgcgtgag aacagatatt gaattgccac cgtgccggca ttcaaccgcg gatcaatgcg
     6181 catggtggtg ccatcgggaa attccaaatg ggtcaatgtg cctgcgcgcc aactgtaata
     6241 gccgatggcg aggttgttga tagcccaagt caattggacc gacatgcctt tgtagtgata
     6301 gtcgttgaaa cccattgggt aatcaatgtg caattcgtcc acgggttgtc cgcgtaccca
     6361 acggctttcg aattcaagca tggccaggat cagccgcggg ttgatcgaat tttcggtggc
     6421 aagccgttca atggcggcat cgccatcgat ccagcctgtg gaaccgagat aatccttgaa
     6481 cttgctcaaa tatccgcccg attgttgaac gtactccttc acgtcaaaat caaccgccgt
     6541 gtaggagaaa atgagctcgg catccggcat gatctgcaca tagggtgtca tcggttcagt
     6601 gatgcgattg gggatgacca gtagcgtacc ggggtcaatc agggtggtct tggtcaagtc
     6661 agcatctgag gtgatctcgg attcgtccac cccaaaacgg tttgccacag cggagagcat
     6721 gtcgccgctt tgggcatagt aaaggaaggg ggccacatca taggccagtg tgtaggtcgg
     6781 ttcaatgaca ggcgtggaac tgtttggcgt gatggttggg gtcggggtat aggcgttttc
     6841 tgtggcgatc tcaggcggaa cggttgaagt agggaagtcg atcggatgat cctgtattgc
     6901 aaatgggtca atgggtgctg aagccagatc cacggtgttg ggcgtgggcg tttgtggaac
     6961 cccccaaagt gagggtgaag aagacatgca agccgataaa accagcgata gtacgatgag
     7021 aatggcaagt gtgcgagaca tggttaagat tatatccgcc tggattaagt tcagatgaat
     7081 taacgataga cttgatttaa ttcgttggca ctatcgagag cttcgattgt aattccgttg
     7141 cgtgagagat agccatcata ttccctgccg ccgccgcttg agatccagcc atcgaggttg
     7201 aagggcagcg gaccatccgc agcgatccac tcgccgttgt atttccgcgc aagatgtaaa
     7261 tgggtggcgt tcgagattcc gccttcacat gaagcatggc cgatgggttc gcccgcatag
     7321 acatactcac cgggctccac acggtcttcc tctgcgacgt gcatgtacag gattacccag
     7381 cctgtttgct cataaccgtc attatcgaga tcctggatca aggcgccgtt ggaggcacgc
     7441 aaaataaggc catccgccac agcccgcacc caaagcggac tgaccccaca accgccatta
     7501 tcgccggggg gagcaaaatc cagcgcggcc caggctgagc cgtaatccca gccgccgtgt
     7561 gggccgccgg tgaaatacca ggtttcgcct tcgctgaacg ggagctgcat tgtgggttgt
     7621 gaaacgtatg caggtacgag gggttcaatg gcgaaatcga atggattgcc aaagaacaca
     7681 taataagaaa gcaaaaggcc tgttccactg acgtcctgct cccaaacggg tcgatcatcc
     7741 agttgggcaa agaagtattg gacggcagct gtccctgcgt taatggttgg gtccaccggg
     7801 acaaccgttc cgtcgttcag aacccaggtt gaaatggcat ttgctttcca aagataatag
     7861 ccctggttca gggtgtttgc cgcccaagtg agctgacgat acaagccata atggccttca
     7921 tctgccaatc ccatggggta ggcggtgttg aagggttcag ggttggtgac ccatccgctt
     7981 tgatattcga gcagggccag caacaggcgg ggattgatcg agtaatttcg ggcgacgatc
     8041 caaacgattt catatcctga gaggatgaat ccgctcacat cctgttcata gatttccaga
     8101 taaccgttct ggctttcgat gaattcctga atattaaagt aggcactgcc ggggccgtac
     8161 accatttctg agtcgggaat cactttgaag gatgttccgc cttcgctggg gttgggtgca
     8221 gggacattca aggtcatacc gacatccagc atatttgcat cagtgagatt gttggatagc
     8281 atgaggtctt ccacgctgat cccgtacatg aaagcgatgc cgcccagcgt atcgccaaat
     8341 tggaccacgt attgcgcctc atcctgtctt ggggttggga ggacacgttc cgcatcggga
     8401 gtgggtgtga cgattggctg gtcggtggga actgcggttg ggaggatgat cgtcaacgga
     8461 acccgtgtcg gggtcggctc ccgcgttgcg gtaggcggaa caggagtgct ggtaatggtg
     8521 actgtgggga tgttgggaga aagcgcaaat gtcccctccg aaaccggcaa aaaaggatcg
     8581 taggtagggt gggcggctgg tgttgctgtg gcaggtgcgc atgaaacaag gatggccgcc
     8641 aacaaatgga gaataatgct tcggaaaaga aacttgaaag acgatttttt ctgaacagac
     8701 atggctgcga ttttaccagc ggagaggaaa agagtgggat atggtgcaat gaaaaagaga
     8761 cgcaaaccgt atggatgcgc ctcttttata aaccgatttt accgaaacgc ttacgactgg
     8821 aaccccgagc ccatgatgta gttttccaat ccacggatct cgctttcctt gttttccagg
     8881 gctgcgaaca tcacatcgcg catggagacc aggcccacca actggtcgtg ctcaaccaca
     8941 ggcaggtggc ggatgttgta ttgtttcatc aatgccatgc actgctccac actggtgtct
     9001 ttggtaacag tgatcatact ggagaccatc acatctttga gtaaggtgtc ttttgcggca
     9061 cggccttcgt tttcgccttt gtaaagataa tcacgttcag tgtaaatgcc aacgatcttc
     9121 ccgccatcgg tcaccaacag ggcgccgatg tgcaactcgg ccattaatcg aatggccttc
     9181 agaacggtat cggtggatgc aattgagtag ttttttgagg tttccttcgc ttccaaaagt
     9241 ttacggacag tggtcatggg tgtcactcct ttaaatggtt ttggttgtta cgactgctgt
     9301 cagtatagca aagaaacaat ggcatgaaat caaactagtg attgatgtct atgacatttg
     9361 caaccaattc cagatcaggc ggacggcctg cgggaccagc gcttcggttt gagcggataa
     9421 ctgccgcgaa aaaagaaatt gatagccgcg caccgagagc agtacagcct cgggtttgcg
     9481 ctgataaagc gactcacaga tcgacaccag agattgcggg gtgagatggt gtgtaaaagg
     9541 agaacgctgg aaatcactct ccacttcgat caaccgtact tcttctggga tgctgccagt
     9601 gtgtgcatcc acaaagcaga cttgttcaaa agccgcgata tcttcggcca tttcaggtgt
     9661 caattgcaaa tgaaatgcga attcgattcc cccgttctgc ggaaattcgt cctcataaga
     9721 ggcgggggcg ggcagcccca gctttagggt cagtgctcgt aaaatatgcc aggccacccc
     9781 atcgtcttcc cggtcagggt ttccgtaccc tatgatcagt gtttttttca agttatatat
     9841 tccggcggac cagaatggcc cgccgttcta cctttgcttt ttaaaatgtc ggccagttgg
     9901 ggctactcgc cgtagcccgt accgagaatg tagttttcca actcaacgat ggtgcctttt
     9961 tgttcctgca gcagcgcctc ggccagcctg cgaatcgaaa ccatgccaac cacacggtcg
    10021 ttatcgagca cgggcaggtg gcgcacacgg tattggcgca tcagttccgc gcattcctgc
    10081 agggtggttt ccagcttgat catgaccatc tggctggtca tcacatcgcg gacgatggtg
    10141 tcctttgcga acttgccctt cacttcccct tcacgggcat agtcgcgttc ggtgaagatg
    10201 ccgatgatct tttcattctc tgtgacaagc actgcgcctg tattggcctc gttcatcacc
    10261 tgcagcgcat ccagtacggt ggcatcggcg gcaatggtgt gaaccttggt ctcggctttt
    10321 tcttcaacta tttttctaac tgtcgtcatg gttggactcc tttgacagaa tttgaggaac
    10381 gatgagcgac aatatttcta aaattgagct ggctttgcgc ttcaatgggt aaaaatattc
    10441 tagtctcgtt tcaaggtctt gaccagtgtg ccgtcagcgg cgacgatatc caactggatg
    10501 ggcatctgcc cgacggcatg agtggaacat gacaggcacg ggtcatacgc acggatggct
    10561 gcttcaacac gatttagcat tccctcatgg acgtctggtc ctttgacgta ggtcttggcg
    10621 acactatcca cagcatgggt catggcccaa ttgttgtgtc ctgtggaaac gatgaggttc
    10681 accttgagca gttttccatt ctcgtcggca gtgtaatcgt ggatgagggt tccgcgcggt
    10741 gcttcgagta cgcccacgcc gtggcctgtg tagttcctgc gagtgttgag gatatcggtg
    10801 ctgaggatat cggggtcttc acataacacg cgcacacgct ctgcagcaaa caacgcttca
    10861 atgaggcggg cgtagtgata gtagagtgtg ttttcaacag gcttgccatt gttcaggctc
    10921 ttgaagatct tcagttcacg atctgccagc ggcgtatcaa ttttttcggc cagattcagg
    10981 cgtccgagcg gaccgacgcg gtacactcca tcggggtagc ccatcttttt gtagtagggg
    11041 aatttcaaat acgaccacgg ctccacatgt tcggcgatca catccagata attggcaacc
    11101 gggaagcgct ccatttccac gccttcacgg ctgatgaggc ggatattgcc atcgtatagt
    11161 tcaagaccgt tttcaggggt ggtcaggccg aaatagcctg ttgggaacac ggcgaacttg
    11221 ttgatgtctt ccatgttctt ttgcgcccag tccttcatga tctgcaagcc tgcctgcaaa
    11281 gtggcaacgg ccgtatccac accactgagg atggaatcgc gttccgccat cgtaagcgct
    11341 ttgttgactc cgcctggcac cgcaaagaca gggtgtaact tgcggccgcc gagcgtcttg
    11401 atgatttcct gcccaaactt gcgaagctgc acggctttga cggtgagttc ggggttggcg
    11461 ccgatcagcc caacgacact tcgaatttca ggagaggcat cgaagccaag caacaggtcg
    11521 gggccagcga gttcaaagaa gtgcattccg tggctctgaa tcatctgacc catgtgcatc
    11581 atttcgcgca acagattggc agggcgtgga ggggtttggc ctgtgagcgc atcgccagct
    11641 ttggcggctg caagatgatg gctgacgggg cagatcccgc aaatgcgcgg agtgatctgc
    11701 ggcatttcaa aatacaggcg cccttcgcaa aatttttcaa atccgcggaa ttcgttgaca
    11761 tgcatgtagg catggtcaac cgtgttatct gccttcatat gaatggtgac ttttgcgtga
    11821 ccttcgatac gggtaacggg ttcaatcgtg atcttttgtg tgctcatatt tttctccaac
    11881 tcaggaaatc cttcaacttt caacctttga ccttcaacct gcaacccata actcaatgcc
    11941 aatgcaatag gtcgtcttta atttcaggct tacgcccctg cgccaattcg gcgaggacgt
    12001 ggaagatggt gtctgcatcg ggcgggcagc ctggcacgct gtaatcgaca gggacaactt
    12061 catggatggc acgtgccttg gtcatggttg ccagttcggg gtcgttggga atttggctgt
    12121 ttggttcgtt gctttcagct tccacataag cgcggcgcag tgcggcttcc acgccgcaca
    12181 gattgcgcat ggcaggtacg ccgccaaaca cagcacaatc acccagcgct acaaggattt
    12241 tggcgcgttg gcgcattttg tgtgccactt cttcattggc gctgttgttg atgccgcctt
    12301 ccagcacacc tacatccacg ccgctttcat cgggttcttt caggtcagtg atgggggtgg
    12361 ctcgcaggtc ggcaagttcc acgatctgta aaatgcgttc gtccatatcc agtaatgaca
    12421 tgtgacaccc agcgcagccg cacatccagt ctgaagcgat ctttggttta ttcatgagag
    12481 actcttttcc ctggcaatta attcaatgca acgcttgatc tgcgtgccag aatggctttt
    12541 tgccaatcgg ttttgatggt ggtattcgtt cgcatcgcaa gttcattcaa gatggcgagg
    12601 ttttcacgga cggtttcggg cggcaccaga gccttgtggc tttcctgaat tcgtccatcc
    12661 aggttgatgt agtgcccttc ctgttccgcc caaatggtga caggtaaaac gacgtctgct
    12721 tgttctgtca gtttggattc ataactggct tgcaccacca gatgcgacgc tttggaaact
    12781 cgttccacca gagatttgga gatgtagtcg tcaccgatgg cggcatacac aactttttct
    12841 ccattcagtt cgaaggcgct gtccatgctg agcagggccg cggccaggct gttggcttca
    12901 cctttgatgg agagcagtcc gtggcgttcg ctatccactg cgccgatcaa cacggcaagg
    12961 cgatgcaact cttcaacgat ggtttcatca cgttgggcag tgacgccttt tccgaacagg
    13021 atcactggcg aaatggagcg tgccaataac cgacccgtct gcctgagttc ttcgagagaa
    13081 acacccgagg cgctgacggc tttttcgatg agctggcctg catcgggtag ggaaagaggc
    13141 gcgcgcccca attcctcctg aacgatgacg gcttgcaatc cgcggatgag tgcgaggtct
    13201 gagccgggtt gggtcttcaa agcgatattg gccatgtaat ccagttcgct tgattcaggg
    13261 tcaatggtga tcaggcgtgt tcccttggaa agattgcgtt tgaacatgaa gcccgccacc
    13321 atgtgactgc gatgcagatt cgcaccgatg cacattaccg tatcggcatt gcgtaaaaca
    13381 tcgagcttgc cttcgaaggc ttcgtgggtt tgtgcaaagc gggtgacaga cgcggtgggt
    13441 acgccttctt ccacactggt gaccagtttg ctttggaatt tttcagagaa cagttctttg
    13501 aaagcggaaa gggtttcggt ggataatctc gttgaggcaa tggccgcgat gctgtttttg
    13561 ttttccatta atttgcctgc tacagcgccc atggcttcat cccacgagac gggctcgagt
    13621 ttgccgttct tgcggaccag cggtgtggtg atgcgtttgc gagattctgt gacggggtcg
    13681 taccgcccgt gctcacataa cacgccgcga ttgaccttgc cgtcccaatc tccttcgatg
    13741 cggacgatgc ggttatcgcg taccatgatc ttgatcgaac atcccactga acagcctgag
    13801 caaaccgact tgatttcttc gaggtgtttg tcgtgtccct ggtaggcgct gactcggtcg
    13861 atcaatgcgc ctgtgggaca gacctgtaca caggaaccgc atttgatgca ggtgctttca
    13921 ccaagcggca catcggtatc ggcaacaata atgctggccg cgccgcgttc tgcaacagag
    13981 agggtaaagt tgcctgccag ttctccgcag gcacgcacac accttcggca aaggatgcag
    14041 cggttgttat ccaaaataaa gtatggatgg gtggtatcca ctgcgaaatt gctccagcct
    14101 ggttgaatgg gccagtgggt catttcttcg tggtaggctg agttttgcag ttcgcaatcg
    14161 ccgccgctga cctgacagaa cgggcagaaa tgattccgtt cactgaacag catggaaagg
    14221 atgaattcgc gggattgctt caaggctggg gtgtttgtgc gaaccaccat gccgttgttt
    14281 accggtagtg tgcaggatgc catgggcaca cgtgcgcctt ccacttccac tatgcacagg
    14341 cggcagccgc catagggagt gagttccttg tggtcgcaca ggcgtgggat gtggatccct
    14401 gccatttctg cggcgcgcaa aaccgttgtt ccatcgggca cttcgatctg tttttcgtta
    14461 atcgttattt ttgccataca tacctctgtg ttgaagttct ggtttcaact attttttgaa
    14521 tgaaaggttt taaaccttct cgatttcctg tgccatttcg catacaccag tcggacaact
    14581 gccagagcgg atgtgagctt ccacttcggt gcggaaatgt ttgagcatat cgcggatcgg
    14641 cacggtggcg gtttgtccaa gtccgcagtt ggaggcacgt tccatttcgt tggcgagaac
    14701 gagcaatgta tccaattctt ccagcctgcc ttgtcccttt gaaattcgat ccacgatctc
    14761 ataggcgcgc tgtgtgccga cacggcaggg ggtgcatttt ccgcaggatt caaagcggaa
    14821 gaattgcaaa agcactttga caaggtccac gatacaggtg ttctggtcgc agatgagcaa
    14881 tgcgcccgac ccaagcgaaa cacccgcctt gcggaaggat tcaaaatcca tgggtgtatc
    14941 ttgcaaaaca gcgggaatga tcgagccgct gcttccgcct gtttgtgcta gtttgagcgt
    15001 gctgccgggt ttcattccct tggcatagat gttgatgacc tcacgcaacg tcacacccat
    15061 gggaacttcg atcacaccgc tgaaattgac attgcccatg atggtgtaaa cctttgtgcc
    15121 cgggctcgaa ggcgtgccaa tggagcgata ccattcggcc ccattcttga taatggcagg
    15181 gacgttcgcg agagtttcta cattattaac tgatgtgggt ttgttccaca aacctctggt
    15241 aacggggtag ggcggccgtg accgtggttc accgcgtttg ccttccaatg attcgagcag
    15301 ggcggtctct tcaccgcaca catacgcgcc cgccccggcg tggatgtgaa tatcgaagtt
    15361 gaaatttgtg ccgaatatgt tttttccgag aaagccgaat tcccttgcct gcagaatggc
    15421 tttttcaagc cgtcgatagg caagtccata ttcacctcga atatagatat aaccttcatc
    15481 ggcgccaagt gcatatgcgg caatcgccat cgcttcgagg atgacgtgtg gatcgccttc
    15541 gaggacaatg cgatctttga acgttccggg ctcactctca tctgcattgc agacgatata
    15601 ctttttgctc cccgcagcag cgcggacgaa cttccacttg cgcccggtgg ggaagcccgc
    15661 tccgccgcgt ccctgcaaac ctgatttctc aaccaggtcg atcacgcctt caggcgtcat
    15721 ttctcccagt acttttccaa gggcttcgta accatttgcc gtgatgtatt cttcaatgct
    15781 ttcagggtcg atacgacctg cacgctccag tacaatgcgc tgttcggcgg ggttgtagcc
    15841 tttgtgtccg cgcagccagc cgatctggcc ggtcagcatt ttgggaggtg ccagcaggtc
    15901 attgacaacc cgtcccttgt ataaatgttc ttccaccagg tagcgcacat ctgcagcttt
    15961 tacagggccg tacaccactg cttccgggta aaccaccacc agcggaagaa tgttcgagtg
    16021 gttgacatcc gagacggtgg aaacatccac ctcatcctgc agtccgtagg tgacgagggc
    16081 ttcgttcaaa tgacgcacaa tttcattggc gcccaggcgg atactttcgg ggtcgttgga
    16141 gaccaggatc atggctcgtt tcttaatcat gttggcctct cttattcgta tttttcaaga
    16201 atctctgtga tctgctcagg ggtgacattg ccgtacatgt catcatcaat gagcaagaca
    16261 gggcccacac tgcatagacc aaggcaggaa gtggttacca aagtccattt gttgtctggg
    16321 gtggtttctc cgttttcgag attgagcttc tccctcaacc catcccaaat ggcttttccg
    16381 cccaccacat ggcagggtgc attctcgcaa aacttgatga catggcgtcc gcgtggtttg
    16441 gtggaaagca tgtcgtagaa actggtcaac gtgtagagtt gactttcggc aatatgcacc
    16501 tgacgggcgg gatcgtggag ctgcttgcgc acttcacgga tcgcttcacg gggcacgtat
    16561 ccaaattggc ggttgatctc gctcaagacg gggatcatcg catcccgtcc gttgccatgt
    16621 ttctcgattg cagcctggac gaccgagacg acgtctcttt caatggttgg ggcagtcatt
    16681 ccttctccga tcacatgatt ctcgatttcg atttaggatt cgacagcaat tgcgttgaag
    16741 ttacagactt gcatacaaat tccgcagcgg atgcaggtgt cagggtcaat atgatgcgcc
    16801 tgacgtctct caccgctgat ggcttccacg ggacaattcc tcgcgcagac ggtacaacct
    16861 gtgcaggtcg gcgcctgaat ttcgtaatgg atcaaggggc ggcagacttt tgcagggcag
    16921 cgcttttcat agatatgcgc ttcgtactcg tggcggaagt gcttgagggt gctaagcaca
    16981 gggttgggtg cgccctgacc aagcccgcat aaactattgc gtgccacttc gtgacaaagg
    17041 ttttcaagct tttcgatgtc gccatcctgg cctttgccat cacagatctt ttgcagcagt
    17101 tcaagaatac ggcgtgtgcc gacgcggcat ggggtgcatt ttccacagga ttcagcctgc
    17161 gtaaaatcca tgaagaagcg ggcgatatcc accatgcagg aggtttcgtc cattacgaca
    17221 aggccgccag aacccatcat ggagcccgct tgagtcaatg cttcgtaatc gagaggcagg
    17281 tcaagatgtt cttcggtcag gcatccgccc attgggccgc ctgtttgtac ggctttatat
    17341 tttttaccgt ctttgattcc gccagccaca tcgaaaatga tctcgcgcag ggtgatgccc
    17401 atcggcactt caatgagacc tggtttgaca acatttccgc cgaccgcaaa ggtctttgtg
    17461 cctttggatc gttccgtgcc catgctggca taccatgccg cgccttttag aatgatctgc
    17521 ggcacgttgg aataagtttc cacattgttg ttgttgctgg gtttacccca cacacctttt
    17581 acagaaggga agggcgggcg tggacggggc tcgccgcgca tccccatgat cgaagccatc
    17641 aaagctgttt cttcaccaca gacgaaagca cccgcgccga cacgcacttc aagatcaaaa
    17701 ttgaaatcgg tgccgaggat gttgttcccg aggaaaccaa actcgcgtgc ttgtttgatg
    17761 gcaatgttga gcgtttccac tgccacgggg tattcggcgc ggcaatagat gtatccataa
    17821 cttgcgccaa ccgcatacgc gccgatgatc atgccttcga tcactgaatg cggatctcct
    17881 tcaagcacgc ggcgattcat gaatgcgccc gggtcgcctt catcggcatt gcagaccatg
    17941 tatttggggt tatcctgcac ctgacgggtg agcccccatt ttctcccggc ggggaaaccc
    18001 gcaccgccgc gcccgcgcag accggaattg ctcacttctt caatgacctg ttcgggtgtc
    18061 atttcaaaca acactttgcc aagcgcctga tacccatctg ttgccagata atcttcaata
    18121 ttgttcgggt cgatttcacc gcagttgcgc agtacaaccc gcacttcctt cgggcgcagt
    18181 ggaccaagtt cttcatccac cacaggcttg gtcacttccc ggaagcgctc cacgatgcgg
    18241 cccttcagga aatgttcttc cactagaaat ggaatgtcat ccgcggtcat gccgtgatag
    18301 tgaacgccct cagggtacac catcatttca gggccgtcac tgcatccgcc gatgcgtgag
    18361 gtttccaata cctggatctc gtcaatcaac ccctgtgcga caaattcatc ctgtaatgcg
    18421 tcaacaactt catgagcgcc ttttgcaaga cactcagggt caacacacac caaaacatga
    18481 gaacgataaa acgccataca aaactccaaa actcgatctt gtaataacgg ccatggccgt
    18541 tacacagcta gctcggtaaa acgtaatcct ttacgggctg cccattttgc acatgctgct
    18601 tcatgatctg cccggccact tcggcattca ctttcccgta aactactttg ggttgatcgc
    18661 cgatcaccac ttgtacgatc ggttcctgct cacacatacc gatgcacccc gtttgcgtca
    18721 cagtgatgcc gcttaggtta ttcgtttcga tatagttcaa cacactcttc atcgtgtcgc
    18781 gtgcgcctgc tgcaattccg caggtgccca tggcaacgat cacctgcaca ttgcccgggg
    18841 cggctttcat ttttttcttt tgcaaggcct cttcgcgaac gcgcttcaat tcttccagtg
    18901 atttgatcgc aggcatgatg ttctcctttt agaaatttgt ttgcagcgca gaggcttgaa
    18961 tgccctcttc gataagcccg cgtacaaact tcaagatttc gggttcagtg agcgacatgt
    19021 cgcccagttc gttttttaat tcggcatcgt cgaattcaaa gttatcgtcg ttgccgtttt
    19081 tgtctgtggt tttgtaatcg aatacccaat gaatattcgg ataggacacc agcaaagcca
    19141 gaaatgtgga tgcgaggtcg ccgagcggca tccggtcaat gtggctgtga cggaaacacg
    19201 cctcgatctt tgtgcctttg cctgcttctg ttaccaggct taatctgccc ccggagattt
    19261 ctgccgccag cttcaaaagc ggaatcccca aacccacttt gcgggtggtg cgtgtggtgt
    19321 agaagggatc cattacctgt ctggcggttt cctcatccat gccacgacca tcgtctgaca
    19381 ctgttgctgt cagcaggtca ccttgcaaat cttcgtggac agcgatgcgg atgttgcgtc
    19441 cgccagcggc gacgctgttc tctgcgatat ccattagatg cagggcaatc tctctcatga
    19501 cggcttttgc accttcttca aaacctgggg gaacttgttc gggcgcaggc gtccctgcac
    19561 ttcttcgtcc accaccacaa caggcgcctg actgcatgct cccacacaac gacattcttc
    19621 caacgtgatc tgcccatcgg gggtggtttc gcccaactcc acgtttaaca tttgtttggc
    19681 tttttcgatc aactgtggaa cccccgccac atagcatgct gtgccaagac aaaacttgat
    19741 ggtatgtttg ccgcgcggtt tggttttgaa gaacgaatag aaagtgacta ctccatgcac
    19801 ctgcccaagc ggaacgccga gttgacgggc aatgtaggct tgcatggaag gggagacgtg
    19861 gccgatttgg ccttgtactt cattgagcac caacattaca gaaccggggc ggtctttgtt
    19921 ttccgtaatc gcagtatcta tgatctgttt ttgttccgcg atgctgagtg ggtcagtggc
    19981 tgggatttcc ttgagtgatt gcgtcattgg ctttaccttc gataaatatt gtgataattt
    20041 tcacaaatct agtttatcat acagtttcac agcgtcaatg accaatgtca tataaagact
    20101 ggtgaaggtt gtcagtcaaa ttattacggc tcgatattga cggttcgctg gctttcattt
    20161 cgaaaagcca tgcgaatttc tgccaacgat ggttgatcga ttgtaaagat cgttttgcca
    20221 agaaattcat cgagcctgtg aacgtcgcca ctttggatga ggggagtgtt tttgatgaat
    20281 gggtagagag aggctgcttt ttcaggggag atgtggcggg aaatttcaag ggaaagaatt
    20341 ggccaattgg gtgatagcag gcccaaggtt gggaaaagac cgtagctgtt cctatccaca
    20401 tgcgctggga tgacgagtcc gcctaacgca ttaactttat tgaagatttc gtcgatcgaa
    20461 aaatgcgtgg acgtaagcag catgcgcggc tcactgcgaa taaagttgcc ctcttcatcc
    20521 acaatgaatt gttcccctaa aaattcggcg ttgtttttca ggttgggcag tgcggaatct
    20581 accagggcct gccagttggc gagtgcttct gtgtggtcaa acaaacagag aagatggaca
    20641 tcttcacggg tctgcacttc cattcccccg aatacgtgta gtgcggttcc gtgcgctgct
    20701 ttttgaacag cagcgacgtt tgcgctggcg ttatggtctg tgatggcaat gatatcaatc
    20761 tggcgttcga gcgcttcctg cacgatcaac ggtgggatca tctccacttc ggcacagggt
    20821 gaaagtacag tgtgtacgtg caggtcggcg cgaaaatttt tcatgccggt cagtgtttaa
    20881 ttgcggaggc cgatttccca aagttttcca tccacttcaa agctgggcat ggaagtggaa
    20941 agcaggatca cgccctgttc gttggctttt gtgatcgtgg cggcatctgg ctgtgcatct
    21001 tctgtaatga tgatcgcggc tacatccagc agggcggcaa cggctacgat attcaaatgt
    21061 gcctgcaagg tgacccagat atatttactt tttgcacctg ccatgacaca tgaaagcagg
    21121 tctgaggtgt acccgccttc ggggtggatc gtttcaaaat ctcgtggatc agtcagcaga
    21181 ttgaggttga gtcgttcgat gatttgctga aggttcatgg agagtcatcc ttccttgcgt
    21241 gcgttgaaat tttcggcagg cacatgaatc cgcatgatga gttttgtgcc tttgccgact
    21301 gttgattcga gttccatgtt gtcgacacag cgtttaatgt tgaccagccc catgcctgcg
    21361 ccgaatccca tttcacggac ctggtcggtt gcggtggagt acccaggctg cagcacctgt
    21421 tcgacatcgg gaatgcctgg gccgtcatcg gtggtggaca tggtgatgcg gtgcggttcc
    21481 acttcaagtt tgagaatgcc gccatgggtg gtgtgaatga tgagattcat ttcggcttca
    21541 tacacggcaa tgccgcagcg ccgcgcaatt tgtgcatcgg cgccaagtcg cagcagggcg
    21601 cgtttgatgt ggctggaagc gttgcctccg tgtgtaaaat cctgggcttt gatgttgtag
    21661 cgcagcacca gtgtggtgcg gtcggagatg atatcttcga agaggtgact ggctcgatag
    21721 cggcgcactt cttccgcttt gtaatctttt tggagcgcca ccagaatgcc gcgggtgata
    21781 tcgcctttgg tgatcatccc tacgagtttt ttttcttcat ccaccacagg aagacgcccc
    21841 acgtgttttt cggcaaaggt ttgcatggct ttgacgatgg agtcgaaact tgccacggtg
    21901 acaagttcgc gtgtcatata ttgtccgacg gtttccgtca ggtcattgtt ttgcagtgcg
    21961 cggacaatat cttcgatgct gatcacgccg accagtttct cgtcctgaac tacagggacg
    22021 cctgaaatgc ggttgatgcg caggatcttc aagacatccg atagggagat atccgggctg
    22081 gcagtttgca aattttttga catcacctcg tggatcttca ggtcatagga caattcttcc
    22141 acgcggttga tttcgtgggc atcctgatca gtgatggggc gtccatgccg accgtggatt
    22201 tcctgttcgg tgcggattct gttaggacac attggattca aaactcggca gtccggcctt
    22261 gtggaggcgg ccgcacaatt cgaacattcc tagcgggctg gtgattaatg gcatgttttc
    22321 ctgttcggca agttcgaggg tttcagcctg gggctgttta ccgcgtacaa aaataattgc
    22381 gcggaagtcg gccatttggg cggtacgcac cacctgtgga ttggtaagcc cggtcaataa
    22441 aacggcgttg ggttggctgg ctgcaaggac atcgctcatc aggtcggcgc catagccgcc
    22501 ttgaatattg atgttcaggt ccgcttcggt tgtaagggtt gtgccgccta ccaattcgat
    22561 cacttgattc agattcatgg ttaaagtcat acctttctga tttgcttttc aatggataat
    22621 ctttaattaa attattctct agcttatctc ctttggtaag tttcattact cataattcgt
    22681 ggagatgatt atccttcttg ttgtgacaat tttcacaatc aagtataaca gaaaaaagcc
    22741 actcatcagt attgggtggc ttttatgtct ttttccgtga tatggtttca aagtgttttc
    22801 ggatggaaat ttggaaccgt cctatctcct tggttttgcc tgatcctttc tgcttttacg
    22861 gaagaacatt tccctttgta taaaactaac cggtatgatc attgcgaaaa cgcaaaatgc
    22921 aaccgtaagc gcccacgatt cgacagaaag caaaaagcca attcctacaa taacaaaaaa
    22981 agcaatgacg gcagcgatcc attctttggg ggtgggccgt gccatgtaac cagattggta
    23041 tttccacttt tgctggttgg tggggatggg gatgcgaggt cctttccctc cacttgacgg
    23101 aatttgaaat gaaattggcg ttccgttcga tctgtctttt ttagccatgt gtattcctcc
    23161 ttttatagcc caactctttc gccatttgta tacacgttga aacggtgttt tcttgcatac
    23221 ccgatcagtg taattccgta gcgttcggcc atctcgattg aaagcgaaga tggagaagtg
    23281 cgcgagatca ggatcggcgc gttgatctgc gctgcttttt gcagcatctc agaactgata
    23341 cggcctgtgg tgatcaaaat tcgattttca ggccagatat cattcatcag gcacaaccct
    23401 gcgatcttat ccaacgtgtt atgcctgccg atatcttccg cggcgatcag gattttttca
    23461 ccatcagata aagcggatgt atgcacaccg cccgtttcac gatacaggga ctgcgactcg
    23521 aaaagcattt ccaccaactg accgatcgca tcaggcctga ccgtgaacct gttcccgctt
    23581 aaagagacat tcggcttcgc gagaagatcc actgctgtta ccccgcctgt acaccctgag
    23641 gtgcgtctcc atgaggttgg ttgttctgcc gcatggctta gccatacatc cacattgtca
    23701 ccgtgctcgc acacgcggac atcttccacc tcatccattt cttgaataat gccctcgttg
    23761 aagagaaacc caactgccag cgcctccagg ttgacgggcg tacacatgaa gctgatccat
    23821 actttgccgt tcaccgtcaa agaaacgggc atttcaacga tcgtttctgc atcgaaggat
    23881 ttccattttt ggaattcgta ctgctcatag tggattgatt tttgtggggc gttcatctca
    23941 aactccgtat accgttaatc gatcacatcc cagttttcgg gtaggctggg cgcggtccag
    24001 ttcgggttgg gacgccagtt cagccagtct gaattaaaag ggtatccgcg tttttcaagg
    24061 cgggtgaata cttcctgttt ggcacgttcg atctcgttga cccagtccgc gtgtattccg
    24121 ccagcctcga taccttgttg atattcgtcc atgtctttcc agctccactg aaaatcgggt
    24181 tcgatgacga tgtcgaggtc aaggtcgagg gtgtcgaatc cgaggtgtga gcggcggtag
    24241 ggcagttgaa aattgatgta gtaccccaca aactcatcct ttgctgcttc ccagatgtag
    24301 atcgtggaaa aatatttttc gggttcaagc agaatcagga atcggttggt gtgccagatg
    24361 tatttttcaa gtttcaaggt gtttgtggtt gtttctgccc agcgatccca catggacctg
    24421 tcatgggcgt ggtggaggta tccgctggga accatgcatt ctgcgccggg ccaaatgagc
    24481 agggcggttt cttcgggggt gtcttgtacc acacgtgctg attgcatgta ggttggtgag
    24541 ccattgtaca caccacgcag gaggatgtga tcgcctgctt tccacaaatt aaggtttggc
    24601 atctttgttt ttttccgagt tgcggtggac tttgtagaaa atatctggat cggctaaaaa
    24661 tgcgccgaga caggactctg tgcaaaagcg aagtgtacgc ccgcgatatt gagcggacgg
    24721 gaaatatttc gggtcggcat cacggaaaat ccttccgcac agcgccacga attttttgct
    24781 gttttttgcc ttttctttat ccacgctctg tttccgcgat gaacacttcg aatgcgccca
    24841 ctttgaatct gggtgcaaat gatccgccgc gtctttctgc gctggtgaac aggatgcgca
    24901 ggtttgaatc cttgatctcc tttgttcgtg aaaaatcaag ttcaaatact ttgtcggagt
    24961 aattcaagac caccagaact gtttggtttt cagaacggcg caggaaggcg aaatattccc
    25021 tggaggtatt gtggagtgga agatattccc catgctgtaa tgccggggaa cccttgcgca
    25081 cacgcaacag atgcttgtag tagtgcagca gtgaacttgg gttcttgtcc tggtcgtata
    25141 cgttgatgcc gtctttgtaa ttcgggttga caggcagcca ggtattcacg cctgacggag
    25201 agaagcctgc attcgggctg ttggaccatt gcatgggagt gcggttcttg tcgcgagtca
    25261 tttctgcggt acggagcatg gcttcttcag ggtggacacc catttcagtg acaatggtgt
    25321 ggtaatacca ggagcccatg gtgtctttga cgtgtgaaat atcagagagc atgaaatcgg
    25381 tcattccgat ctcttcgccg ttgtacagga atgccgttcc tttgagggtt agaacgagtg
    25441 cggcgttcaa acgtgccagt tctgcgtcgt ggatcttatc gccatagcgg gtgtggattc
    25501 gagagcagtc atgatttcca agggtgttgc atggccagcc tgtttcgacg ggtagttttt
    25561 ccagagcggc aagtctatcc ttttggttct tgcggatgtg tgcaggcgtg atctggtctg
    25621 tgcgcatgag ggggaagttg aagatgagtt gcagttcatc ccgcccgttg cccatgtagg
    25681 cgatgtcgtc atcttcgcca acgagcatgc ggtcgccttc gtattcttcg agaatggcgc
    25741 ggagttcttt cattagctca tgtacacctg gtttcccata ttgatgcttg aacatttcat
    25801 accagtattt gtctttgagc tttttctcct ttggcgtgtt ggcaagctca gagaaacggc
    25861 gcaattccgc taaattcatt gggacattgt gcggggtgag ttgcgggtct tcgaaaatgg
    25921 tgccaatggc atcaaggcga tagccatcca cacccatatc gagccagaaa cgggcggcat
    25981 cccacatggc ttttttcact tcagggttgt gccagttcaa atcgggctgt tgcttcatga
    26041 agtagtgata ataatattgg ccgcgctcgg gcacgtaggt ccaggcttcg ccatcgaagc
    26101 aggattgcca gttgttgggc ggcgtatcca cccagacgta ccaatcggct ttgggattgt
    26161 cgcggctgga ttttgactca aggaaccacg catgctcatc ggaggtgtga ttcaacacaa
    26221 ggtccaaaat gacgcggata ttgcgtttat gcgcttcgtt gaggaaggtc ttgaaatcgt
    26281 cgagtgtgcc gtactcaggt gcgacattgc agtaatcgga aatgtcgtag ccgcaatccc
    26341 agttagggga ggggaagtgc ggagaaagcc agaccgcatc cacgccaaga gaggcgaggt
    26401 aatcgagctt ttccgtggcg cctttgaagt caccgatgcc gtcgccgttt ccgtctgcga
    26461 acgagcgcgg atagatttga taaaaaacag ccgtttgcca ccatttgtgt gcagtcatga
    26521 gttattcctt atagtttgaa ggttgaaagg tttgaaagtt ggaacgttga ggaattgttt
    26581 ccttcaaact tgtcaacttt ccaatctgcc aactattttt acacagtcat gtttgaagac
    26641 ttcttcttcg tgcggggcgt cggtgagttc accgctgagg tcctggcctg ggaagtgcag
    26701 aaattcatgc atgccagtgc gccatttggg gcaatcggtg acatcgtaca cgatgccgtt
    26761 gtgtgcgatc cacatgcgtg agtagcgttc gcctgtgttg cggcgtaatt ccaaaataga
    26821 gatgattcgg tcggggatgt tcatggggga gattatatct ttcgaaacag aatcagccca
    26881 aggttagttt gaagcgggga cgatgaccgt ccgtacgtcg ccgctgaatt tttccagtga
    26941 gtcgagggtt gcgttgatcg catttgcatc cagcggcacg gtgcgggtga cgatttttga
    27001 agtgtccaac acttttcggc gtgccaggtc tacaagcagg ggaagttcct gcagcaagtg
    27061 atcgttggaa ccaattagtt caacctcgtt gccgagcagt tctgtgtagg tttgaatttc
    27121 gagcggtttg tttgaaagcc ccacgatgac cacgcgtgcc atgacacccg cacattgcaa
    27181 agcctgtttc attgtcagag gcaggccgat catttcgatg gcaacgtcca cgcctttgcc
    27241 gtgggtgagg cgtttgatct cctccactgc gttgaccttg tttccattta cgggaatcgc
    27301 gccatattgt gcggcaagat ttaatttgtc atcgttgata tccacagcgt agacttcgac
    27361 cgcaccgaat gcttttgcaa gttgaatggc cgattgtccg agtccgcccg cgccgaaaat
    27421 tgccacggtt tccccagctt tgagtctgct cttgcgcagt gcgtggaaag cggtggccga
    27481 ggcacacatg agcgtggcgc cttgctcaaa gggaatttca tcgggcaggc gaatggcgtt
    27541 gcgtgcgggc acgctgatgt attcggcata gccgccgttg gtgtagtggc cgagcatcaa
    27601 aaccttttca cagaattggt cgttgccggt ggagcaatga taacaatcgc cgcaggagat
    27661 gttgtagtgc aggcaaacgc gctcgccgat tttcaaattg ccaacctgtg cgccgacctt
    27721 ttcaaccacg ccagccactt catgtcccag tgtgagcgga acaggtttga cgggtgaaat
    27781 acccgcacga taatgcgcat ctgaatggca gatgcccgca gctttgacgc gcacaagaat
    27841 atcgcgctcg ccgatttcgg gaatggggat ctcctgcatt tgaaggggtt ggttggcacc
    27901 gataaaacgg atggctttca tgagaggctc ctttgactgg cggggttgaa tttcaaacaa
    27961 aaatcgtttt atttggctgt aattttccag caccaagccg aggtttagat ctttttcact
    28021 gcatcgccga cgcgcactgt gccagcctgc accactttgg cattgatccc acgcaggtga
    28081 agtttcttcc cttcggtgga gttgacaaaa agtgcggcgg cgcgcccgta gcgggcggcg
    28141 aatttgtcac aacctgtatg cggctttgcc gtcacttcaa gaatggcaga accaagcgcc
    28201 agccttgtgc ccgcaggcag attttcgctg cccagatcaa ggtcaataaa aaattgatcg
    28261 cctgcaagtg cccagcggtc tttttcctgt gccaacagtg caaccgcacg tgaattcatg
    28321 atgttgatct gtgcctcagg atgagcagac ccatcgggtg tggcagtact gccgcgggtt
    28381 ttccagttat cgccaaccag gccttccgtc aggttcagct ctgccgattc gagaatttcc
    28441 cttccgtcca cttcgggtct gcggacgatc atttccacaa caccgttgtc tttgggtgat
    28501 tgtgcaatgt gctggaggcc agcttcaagt tcttctttcg tcaggtggtt cggggtcatt
    28561 gtgaattctc ctttataaaa ttatcgcatc gcaaaaatgc aaacgccgtg caggttttcc
    28621 attttcgacg tttgttccac tgagtttccc agcgcattct tcagaatcgc cgcatccatt
    28681 ttacacaact cggggtgccc cgcgatgatc tttgcatagg ggcatcttcc caagatcacc
    28741 cgcgggcctt ctgcgctggc ttcccaacgc gcctgataat gcatctcatt tattttttcg
    28801 attaagagca caagcctttt tgtgatggga agatttgcaa actgacccga attcaaaata
    28861 tgattcgcca gcgcctcgat atttaaccgc gggcctgatt ctgtcaaaag ggctttcgtc
    28921 agcgccgaca aattatctcc caacgccgcc tgcgacaggg aatacaactt ttcagggcgt
    28981 ccacgtcctt cgcggttgtt aacctctgtg gattccacgc gcccgtcaga gcacagcaca
    29041 gaaaggtggt ggcgcacatt cggcgcggac attttcaagg cgcgcgcaat ttcccgtgcc
    29101 gatgcggcgc gggtcttttt caagtgggcg aggactttgt ggcgggcggt gatcatcggg
    29161 gagaaaatgc ctttcgaaac tgaggcaggt atcggtttat gtgatgacct cgttgtcatc
    29221 cgaatggtgt gattatactc acttctttat ttttgcaaat atatttttac gtaatttggt
    29281 tgactaatcc acttcacctg ctataattcg cgcttgtgac ggaaagtacc gaatgacctt
    29341 gaactgggaa tccacctacg ccattgcttt ggagcttcgc cgccagcacc ctgaggtaaa
    29401 tatcgaagat gtttcgcttc aacaaattta ccaatggacg attcaactaa gtgaatttga
    29461 ggatgacctc gcgctcgtga acgatgacat cctttacgca atctatcaag actggttcga
    29521 ggagaacatt catggaaaat gaaaagctaa gtttaccgaa ctacaacatc cctgaagacc
    29581 ttgaaaatgt tgtagccatt acgacgctcg atcgtttgta taactggggt cgtcgttctt
    29641 ccgtgtggcc gttgatgttc ggtctggcat gctgtgccat cgaaatgatc gctgcccaaa
    29701 ccgctcgtta tgacatggcg cgcttcggca tggaagtgat gcgcccgact ccgcgtcagg
    29761 ccgatatgat gctggtctct ggcactgtca ccaagaagat gctgcccgcc atcatccgtc
    29821 tttacaatca gatgcctgag ccgaaatatg tggttgcgat gggcgcgtgc gcatcgagcg
    29881 ggggaccttt caaggagggc tataacgtag ttgctggtat cgataaattc ctgcccgttg
    29941 atgtgtacat accgggctgt cctcccactc cgcaggcttt gatcgctggc ttgatcaaac
    30001 tgcaggaaaa gattgacaaa caatccatca agaccgtgcg ttggtacaac aaggaaaagc
    30061 aggatggtaa ctatgtgccc atgccgatcc ttggcccgga cttgatcgat gtccgccgca
    30121 acaacgagat caagcaggcc gcagctgctt cgcaaaagga aggtgcataa tggccaccgc
    30181 gatccaaact tccaccgttg atctggttgc ccgtttcccg ggcaaggtca ctgctgatac
    30241 gcgtcccggt tacagcggat ttatcgttaa caaagacaat ctcgtagaag ttgccactgc
    30301 catccgcgat gaatttggtt ttgacctgct caccgccgcc accggcgcgg attacatcgc
    30361 cgaaaataaa atggaaatgg tctatcacgc ctacaagacc accggcgggc ctgggttggt
    30421 gttcaaggtt caggtggagc gtgttgaccc cgttgaaatt ccgtctctca tcaatatctg
    30481 gccaggtgtg gattttcagg agcgtgaagc ctgggatttg ctcggaatca aattcaccgg
    30541 gcaccccgac ctgcgccgta ttttgatgtg ggaaggatat gaaggccatc cattacgcaa
    30601 agattggaaa gaacctttct ttgaagagga agccaaacct ttcaagagcc gctggccgga
    30661 tgggcagcat acctttgctg aaaacaagaa tccgttccgc gataatttga aattccccgc
    30721 ggatttcgac cctgagaaat ttgagatcgt caacgaagac ggactgtatg cttctctcga
    30781 acgccacacc atgaagaacg aagagggcag ccttaacaca gaccatatcg tggtcaacat
    30841 gggcccgcat catccttcca cacatggtgt gttgcgcgtg gctgttacgc ttgatggtga
    30901 aaccattatc ggtcttaagc ctgtgatggg ttatctgcat cgtaaccatg acaagatcgg
    30961 cgagcgcaac acctatctgc aggtcattcc ctacactgac cgcctcgact atttcaactc
    31021 gatgagcaat aacttcggtt atgtcaccac gatcgagaag ttgatgaaga tccaggttgc
    31081 ggagcgtgcg gaatacatcc gcgtgatcat ggctgagtta agccgcgttc agaaccactt
    31141 gatcttcatc ggcatgttga tgaacgatct cggtaccatg tacactccct cgctctacgc
    31201 ttttgaagag cgcgaactgg tgctcgatat ttttgaggcc gtttcaggcg cacgcatgat
    31261 gtgcaattac ttccgctttg gcggtgtggt gcgcgatatt cccgattatg tgatgcagaa
    31321 gatcaaagac ctggtcttcg agcgcttgcc cgcgaaaacc gatgaaatgg aacgcttcct
    31381 caacgaaaat gagattttgg tggcgcgcct caaagatatc cacgtgttga atgcggaaga
    31441 cgcgatcaag tactcggtga cgggtcctgt gcttcgtgcg gcaggtgtgc cgtatgatct
    31501 gcgccgcgcc gatccgtact ccatttatga ccgctttgat ttcgatgtgg cggtccgcca
    31561 gaacggcgac atgatggata actacctgat ccgtttcgat gaaatccgcc agtccctgcg
    31621 tattttggag caggcactca aacagattcc ccaggggccg atcaactcac agaagccgca
    31681 gtatcaggtt cgggttccgg caggtgaagc gtacgggcgt atcgaatccc cgaagggtga
    31741 gttgggcttc tatgtcgtct cgaacggcaa gccgaatccc tatcgctatc atgtgcgccc
    31801 tgcatctttt gtcaacttgc aggccgttga aagcatgtgc atcggcacaa agatcgccga
    31861 ctttgtagcg ctgctggcca tgctcgatat cgtgatgggc gaactcgacc gctaacagga
    31921 gaatgaacta tgtacggaaa aggtattctt aaaggattaa gcgtcacctg gaagcgattc
    31981 tgggatacat acacggaaga tatttcctgg atgctgcgcg gcaagaaacg ctacaacact
    32041 gttgaaggcg tggcgcatcg ttcaagcaag gacaccaaag gcatctttac ggttcaatac
    32101 cctgaagagc agttgatcca gcccgaagag tttcgatacg tgcccttcct tgtgtacgat
    32161 gaaggcgcag atggaaagaa ggaagtgcgc tgtacctcgt gcggaatttg ctccaaggtg
    32221 tgtccgccgc aatgtatttg gatcgtgagg acgaacgatc ccgccacggg caggcctgtt
    32281 cctgttccga cgaatttcta catcgatatg gacatctgca tgaactgcgg tttctgtgcc
    32341 gaatattgtc ccttcgatgc catcaagatg gaccacgatt ttgatatcgc ctcctatgac
    32401 cgcaatgtgt acaacctcga aaagctgctg aagcccgcca aatattacgc ggagatccgc
    32461 cccgagaatt acacccgcga agaggaagcc cgcaaagcca aggaagccgc caaggccgcc
    32521 aaggctgaag ccgcatcagc ctaaatttga ccgcagacag gtaaacacga atactgttta
    32581 cctgtctgct tgtttacttg tacgcaattt atcactcata ggaaacgaaa tgacactttc
    32641 aaaagaatct gttctcgctg ctttaggcaa agtgcaggaa cctgaactac atcaggatct
    32701 tgtcaccctg aatatgatcc gcaatctgga gatatcaggc gacaaggtct catttactgt
    32761 tatgctcacc actcccgcct gccctttgcg cggacgaatt gaaaaggaag tgcgcgaagc
    32821 tgtgatggcc gtggatggcg tcaagaccat tgacctcaaa atggattctg acgtgccaaa
    32881 cgacggccgt atgcgcgggt tggtttccat gccgattcgc aatgccattg ccattggttc
    32941 tggcaaaggc ggtgtgggta aatctactgt ttcggtgaat attgcagtgg cattggctca
    33001 aagcggtgcc cgtgttggtt tgatggatgc cgatatctac ggcccgaaca cgcccaccat
    33061 gctgggtgtg gataaacttc cgccgccctc tgggcaaaaa ttgatccctg ccgaggcgta
    33121 cggaatcaaa atgatctcaa tgggtttatt ggttaaacct ggtcagccgc tgatctggcg
    33181 tggacccatg ctcaactccg ccatccgtca atttcttggc gatgttgtgt ggggcgaact
    33241 tgactatctc atcattgacc ttcccccggg cacgggtgat gccgcccttt ctctggcgca
    33301 ggctttgccg cttagcggct ctgtggttgt gacccttcca caactcgttt ctcttgaaga
    33361 tgccagccgt ggcttgaaca tgttcaagca gttggaggtt cccattttgg gcgtggttga
    33421 gaatatgtct tatcttgact tgcccgatgg cacccgtatg gacctgtttg gttcaggcgg
    33481 cggtgaagag ttggccaaag ccacagacac caccttcctt ggaaaaatac cgatcgatca
    33541 gaatgtccgc attggcggcg atacaggcaa gcccatcgtt gccgcccatc ccgaatcggc
    33601 tgttgcgaaa tccattcagg aaattgctca aaagattgct gccaaagcca gtgtggctgc
    33661 catgagcgcg aacaacgcat tgccgatcaa tatcgtggaa tgaactgata tgtgaccaga
    33721 cgtcaccttt tggggggtgc aaatcgaagg tgacggtttt atacgaacgt aaaagtgcct
    33781 cgtcttggaa tttcaattcc aagacgaggc actttgttat ttgttagaaa atgtctgaaa
    33841 agtctgtttt tgaccacaac actggcgtgc ggtgccaggg agactcaaag agtaaaaaac
    33901 ttcgtgcttt tgtgtcttcg aggcttcgt
//
LOCUS       DAOQJC010000036        33290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_581_length_33290_cov_10.055183, whole genome shotgun sequence.
ACCESSION   DAOQJC010000036 DAOQJC010000000
VERSION     DAOQJC010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 33290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33290
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_581_length_33290_cov_10.055183"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /locus_tag="PLE39_07420"
     CDS             <1..1445
                     /locus_tag="PLE39_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68192.1"
                     /translation="TKRIFISADHGMAIIYFLQSDVVSTLLEAGVEVIVLTDDDTKEK
                     IASRFARQGLTFEGLRLKQANDYAKKVQPRMQWLLGYLRRVGGSRRINTEAMDSHIWE
                     VWGENGWKFRLGIWIPSALMILLLRNFSWARSLLVRMQNRFTPTPGLYTDLFEKYQPD
                     MVIASTPGWRMDRYLLRESARRGIPNMTVIVGWDNSSSYNVSGADVQWATCWSELQKN
                     ELVKGSDWDPEHVNIGGIPSYDGYFRKQWLMPRDEYFKLHGLDPNRKLISYASSFVHF
                     APNFPNIEALAKLVSSDSLAEPSQLLIRLHPSHFQDKPKIFADERAQVFELEKKYPHV
                     HVVQPVALGGSLGYYGGEDMDEKSSMMAYSDVVVTVYSTMLVETAVHDTPMIAATIDT
                     PGGWNKPNKFSLSLKEIGDWPTHQRFRWAKAGRVAENEEQLRDALNLYLTDSTVDAAQ
                     RRKFVEDEITFTDATSGKRTAEFILKVLGK"
     gene            1538..2152
                     /locus_tag="PLE39_07425"
     CDS             1538..2152
                     /locus_tag="PLE39_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68193.1"
                     /translation="MNKKQLTILSTLILFILSACGGAAEPTITAADVEGTAVANAWIA
                     LTQTQAALPTQTPSPIPSETPTIQPTMTAAALVLPTLAPPTLAVVTTPTNPADDPCNQ
                     PPPAEPKGLTVKVKFVNKSGGQAALSFGMNFPNKIGECGTYGYTLGPFDEPTVTVLAG
                     CYWGYAWITGDKPSVAKTGDAILCVQDPNEVRAIWITAENIGFH"
     gene            2273..2719
                     /locus_tag="PLE39_07430"
     CDS             2273..2719
                     /locus_tag="PLE39_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNE68194.1"
                     /translation="MKPKTMSLFYFSITLAICSSALYHFVAKSTPSGVNFTVSLLVTY
                     AVAFVVTLFTFFFFPTKNGIAAELKQLNWASIGLAIAIVGIEFGFLLTYRAGWHLGIA
                     AVLVNVVASLILVPVALLVFKDEISWVNILGILICLVGLVMLNWKR"
     gene            2716..3246
                     /locus_tag="PLE39_07435"
     CDS             2716..3246
                     /locus_tag="PLE39_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68195.1"
                     /translation="MKHTKFLLILLTALLISCTPTPAPVHFPTFLPTATANAASVPSS
                     VSTATLPAAATVETFYKFINAAQSPNDFNAAWDMLTNEEQCNTPEKCELSYFQEKWME
                     SKVIYKIYECGPDYAIVEERLYPRTADPASASSEARLIRFQLAQADEQIFITNRKTVM
                     ALEAGCVLIVDSALSP"
     gene            3287..4792
                     /locus_tag="PLE39_07440"
     CDS             3287..4792
                     /locus_tag="PLE39_07440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE68196.1"
                     /translation="MKAPFFSSRFAQIAAFVLLLGLGLGIRLYDLTDLPLDFHPTRQL
                     LSALKARGMYYQTLSDIPTEQKSFAVQQWKIRAAIEPEIVERIVAFTYQFTGEQIWVA
                     KVFSSAFWVIGGLFLFLLARQLTSTEGALAATAFYLFTPYAVAASRSFQPDPLMVMLI
                     IIFWWAVYKWSEDLTSFQWAVIAGLFGGLAIYVKFVAAFFVIGGGLGSILRRESLFET
                     ARRPQIYVMTVLGILPGAAYVIYGVWIAGYLGQQFGGRFIPALFLSPSYYLGWVGMLN
                     IVIGGIALMLGLLGLFFVDEKKRRFFLGLWGGYALFGMYFNYHISSHDYYSLPLIPIV
                     ALSLAPLAEWFFAQLTALTPTHLARFSAFGLLLFGIFASLWNIRAEMKATDYRPEAQM
                     WAEIGETLEDKNTAGLTQDYGSRLAYWGWTSITTWPTYGDLIYHDDLRGAQLEFDGLF
                     EDIALKKELFLVTDFDELKRQPLLKEKLSGYPIFAEGDGYIIFDLTSTGSQ"
     gene            4793..6247
                     /locus_tag="PLE39_07445"
     CDS             4793..6247
                     /locus_tag="PLE39_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68197.1"
                     /translation="MTKTTKPTFDFITIATGLGAAFSAFALGVYAYLGIFSRHLADDY
                     CSVDFIRTNFFSALLNNYIFVSDRYTNFMLIALSEYISPRTVSILPALFIVLWLTGIA
                     WMLSEASRFTKNGWSKLTIVSLTFLVVFFSLLQAPNRFQILYWRSAMSTHFAPLVFMP
                     FLAAFILKVITESEGKPAPFWKYLLGFFIALIIGGFSEPTVAVMITLLALAFAATWKW
                     MSPSTRSAALKMIGWSLAGAVTALLIMGLAPANYYRVSLGKEPPTLAFLIVRSFQYGF
                     NFIDNSFRTLPLPTLFTVAMPFFMFYNRYASPTPALTESQRKLARTLLMVIPALSYLL
                     IVASFAPSVYGQSFPVERARFAGQVSLVAGLMIEGALLGSLLAQWRPAFAEKLPLKLA
                     SAVLFAVAALYPLRAGWLSLNDIPDYRARAEAWDTRVIQINDLIAQGQTDLLIVQLDG
                     VEGVKELEVTEKHWVNRCAAKYYEVNSIRTAPPK"
     gene            6250..7170
                     /locus_tag="PLE39_07450"
     CDS             6250..7170
                     /locus_tag="PLE39_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNE68198.1"
                     /translation="MKRPILVTGAHRSGTTWVGRMLAANLQTAYISEPLNVLHRRGVY
                     RAEVQHWYQYITSENEGAYLPAFHELLNYRYHLFAEIRSIRSQKDFLRMGRDLSIFMK
                     GKLQNQHALLKDPFAVFSAPWFAKTLNCQVVITVRHPAGFASSLKRLNWPFDFRDLLN
                     QPMLMRDHLEEDRAEMESTPADDIIGQSSLLWRMIYRAVHASRTRGLIPEIKIVRHED
                     LSLDPIAGYKSLYESLGLTFDENVRDTILNSSSSENPTELSKKKVHSVKLDSRANMDN
                     WKKRLTAEEILRIRKMTESVSHLFYSENEW"
     gene            7197..7976
                     /locus_tag="PLE39_07455"
     CDS             7197..7976
                     /locus_tag="PLE39_07455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE68199.1"
                     /translation="MSTLPLVSIITPSFNQAQFLEATIQSVLGQEYPRIEYIVVDGGS
                     KDGSVDVIQKYAGRLAWWVSEQDKGQTDAINKGFNRATGDILAWINSDDTYNPQAVGQ
                     AVKQLMENPEVGLVYADCNYIDEAGNVIGKFPAAQTDHRRLREGYVHIPQQTMFFRAK
                     YWKELGPLDPSFYFAMDYDLWTRIAKHAPFKYIAGQTWANFRIHNLGKTTSADDRCWP
                     EMIRVHYRDGGSFFSVIVAKHYLRKVIGPLWKWRIKKQANR"
     gene            7999..8604
                     /locus_tag="PLE39_07460"
     CDS             7999..8604
                     /locus_tag="PLE39_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68200.1"
                     /translation="MTHLLTPPTLEDLIRLLKAWRFWMFGALVGALIGAAVYYADPPP
                     FRAQATVNVDFNLEQAWPQETDRQQFYYLERESRKLEEVAWSDEVLGQLSSSFGISVE
                     ELRRGKLQLSQPAEAGWHFYADDDDAQFAASLATTWAKAFVAKTEADVASGAINEFVR
                     LEATQSESTAKERSIPLSSYLLAGSIGFLTLAVFVLLFKRK"
     gene            8670..10139
                     /locus_tag="PLE39_07465"
     CDS             8670..10139
                     /locus_tag="PLE39_07465"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNE68201.1"
                     /translation="MKEFFPNSYKISRILWGAALLTLPVTSFRWFPFLGEGTLVRPLA
                     MYPLAVLIPLLLILAWRGKTKLNWSGALIPLGVLVLFMVAATSFGALIDPIPLRGQTY
                     SGRAIRALATLFIGIAFFIAAMWMNKDEDDFRFTVTWLFAGLCLDLAWSGLQAVTFYT
                     GLLEKEMVTHWQRAFSMRELVRTNRISGLAYEPAWLAGQFATTFIPFLFAAVLTNFRI
                     TRLKWLEPVLLALSLLVVLATYSRGGLLTTLAAAGLTFLFTGQLGIVWKWFIGGFRGG
                     MRGILFRVGILAAIFSVAAGAFIFLSQKNFFRRLWETDAQSLSEYIVDINAGARGAYS
                     AGALSAFEEYPWAGVGLGASGFYIYQNLPDWSLTTVPEIAKQLSPENKLYPNPKNLYA
                     RLLAETGLIGFFLFLAFQFYILGDILSLLRHREGWARFAAVAGIFAWLAITFYNFTQD
                     SLTTPNIWVIPGILVGLAASSTMPEEPLPGIGLQSKENK"
     gene            10136..10861
                     /locus_tag="PLE39_07470"
     CDS             10136..10861
                     /locus_tag="PLE39_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNE68202.1"
                     /translation="MIVLSVGMPRAGSGWHYNLIHDLMKTTGCADARDIRERYRLQNV
                     LTEVNCNIGVLSARRLGLVMIPALVNTFVIKAHSAPTNASRLLSTMGRLRITYIYRDP
                     RDAMLSAYDFGQRALTKGRPNAFSHLSSFEKSVDFMMEYVHIWEKWIKEKNVLTARYE
                     DLLMNYDEESTRLVEYLKLNGSRPEVRKVIEQYRPGTAEGQQGLHFYKGKIGRFRDAY
                     NEEQKSILKEKLGPYLTQMGYNE"
     gene            10931..12682
                     /locus_tag="PLE39_07475"
     CDS             10931..12682
                     /locus_tag="PLE39_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNE68203.1"
                     /translation="MLRRFDSPYMADWFAISLRWIILVGFVVSLGLGDKLEIAVSWPL
                     GLLILWNLFMTALASMNIRVAYHRLFSILVDITLAGLFFWTQGGLRGPAAWAGILPIL
                     TGSIFFELLGAVAASVFFSGLMIYLGLQDGDSLPLSITFSAALIALSMVFGFLGKRMI
                     IHMRKNRELWLDSEERKSAMQAERMRAIYELTAALSSTLSYKRVLESALNLSASALNP
                     DPEQLHSDPLVGVVMLFNGGKLRIGSARRFTTADMRVTFDAAEGILKKTIDEGEPLWF
                     KDIGYDPELGRVIALRNCTSGYCFPLRSGFNVYGVLLFAHPDAEYFTTERRNLLDIIG
                     RQAVIAIQNARLYQDLVEEKERMVEIHEEARKKLARDLHDGPTQSVAAIAMRLNILRR
                     MMAKDVASAGDEVVKLEELAHRTTKEIRHMLFTLRPLILESQGLVAAIQAMADKMAET
                     YSQKVTHNIDERVTSQLEMGKQGVIFYIIEEAVNNARKHAAAETIAVNLTQMDAGIAL
                     LEIADNGLGFDVKSVTEAYDKRANSSLGMVNLRERAELVNGLFQIDSAPGKGTKVQVY
                     IPLTEEAADRLHYMRNK"
     gene            12686..13573
                     /locus_tag="PLE39_07480"
     CDS             12686..13573
                     /locus_tag="PLE39_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE68204.1"
                     /translation="MPIAANLYYFSHDTEDDLETKRPPIILIHGAGGNLLSWPPQVRR
                     MVDERVYSLDLPGHGKSEGTGRQSIDEYVEDVLAFMQALKLRTAIFAGVSMGAAVALA
                     LTLKHPRKVRGLCLLGSGSKMRVAPLILETAGNPNTFESAVDLINENCFSSEAPQHLK
                     DISKRHLLDVRPPVLLGDFLACHEFDVTSQLKKIRTPTLLICGAADKMTPVRYSELMQ
                     ETMPNAKLHIVENAGHLVMSEQPDVVADLMKQFVDSIPVRTRKTKKDRVELNVESDPA
                     QETESALPADSVPSTEPNS"
     gene            complement(13570..14166)
                     /gene="rdgB"
                     /locus_tag="PLE39_07485"
     CDS             complement(13570..14166)
                     /gene="rdgB"
                     /locus_tag="PLE39_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNE68205.1"
                     /translation="MKKLLIATNNKGKVKELQELLRELNVQLVTPAEINLDLDVEEDG
                     ATYIENAAKKAIAFAQASGLISLADDSGLEVDALDGAPGLYSARYHPKPGANDADRRA
                     YMLENLQGKPRPWKAHFHATIAIAIPGGDVKIADGNCHGEIIPEERGTGGFGYDPIFF
                     MPELGKTMSELEMDEKNRLSHRARAVINAMPVLKEIFS"
     gene            complement(14163..15221)
                     /gene="queG"
                     /locus_tag="PLE39_07490"
     CDS             complement(14163..15221)
                     /gene="queG"
                     /locus_tag="PLE39_07490"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNE68206.1"
                     /translation="MSDLKQAIKDKARQLGFILAGVTSCEPPAHYGMFEEWLHQNRHG
                     NMQYLAEERSRIRRADPKQILPECRSILVLALPYQPVTFNPQSSREAFRISSYALGDD
                     YHDIIPLRLQQIVDFIEEQLGHPIPNRYYTDTGPILERELAQRAGLGWIGKNSMLINP
                     QMGSAFFLAEILLGIELEPDDALITDHCGTCTRCLDACPTQCILPNRTLDASRCISYL
                     TIELKEDIPEELRPLMQNWIFGCDACQQACPWNRFSPQADPAFESKIPLPVLTSDLLL
                     TSVEFNQRFKKSPVKRAKRRGYLRNVAVAVGNTGSENDLPILEQAAQDEEPMIREHAQ
                     WAKKHVTQDIILRHRNSK"
     gene            complement(15262..15876)
                     /locus_tag="PLE39_07495"
     CDS             complement(15262..15876)
                     /locus_tag="PLE39_07495"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNE68207.1"
                     /translation="MKARFVISAIIATLMLAACAPATPIVPTPDVLAVRTSAAMTVVA
                     EFTLTAAAFTPTPMPPTPTFTAEPPTLTPTIAFATDPTQIALGTPGGPLCDDYSFDVA
                     TVDVTILDGTAMTPGQEFVKTWKIKNTGICAWGDGYGLVYTYGDKMSGVPVPIGLVEV
                     GQEVEVSVNFKAPDQIGEYTSAWQMANAKGVTFGKAIFVKIVVK"
     gene            complement(15904..16605)
                     /locus_tag="PLE39_07500"
     CDS             complement(15904..16605)
                     /locus_tag="PLE39_07500"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNE68208.1"
                     /translation="MRVRKFLWLASFLVLAMVLSSCNLGATPVPTQDLAAVQTEAFNA
                     VLTQVAAQSSPTPLPTNTLLPTSTLAALPTFAPVGGGDATPIPFNTQQPVLGITPLAS
                     PIPTAAVFATITTKNGCNDGVLVKESEPFDGDVLQRGKAFEKVFQFLNTGSCVWDEGY
                     TFAFLPDFSTPGFKGSDIVIKKAEDYVKPGANISFVLKLVTNNAPGEYIGTWKLRDDE
                     GNYFGSMVWVKYVIK"
     gene            complement(17069..18313)
                     /locus_tag="PLE39_07505"
     CDS             complement(17069..18313)
                     /locus_tag="PLE39_07505"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNE68209.1"
                     /translation="MKKAPISLFVTFLMMAQLACNLGNSTATPDTFATLNGLYTASAQ
                     TLEAGTTQQVSTAAVGASATPGLPLPTYSPAPPPTGLPAATATNQPIYATAVPVSRCD
                     AAEFVKDITYPDGSPVVRNTSFEKTWRIKNIGTCTWTTAYSLVFDGGYAMSGPTTAAL
                     SKSVSPGSTIDISVQLTAPSAEGEYIGYWKLRNANGVNFGIGDKAQKAFWVEIRVPGA
                     SYMAYSFVEHFCEAEWRNEGAPLPCPGTRGDANGFVVRLDTPMMENGVTENNPSLLTV
                     PQDARQGEISGQYPAFTVKEGDHFRTIVGCQYGSRRCDVVFRLDYRNNGTVKNLFTWN
                     EVYEGNSYPIDFDLSPLAGETVKFILVVTAHGGQNQDDAIWLHPFILRQGTPVPTTIP
                     TLTFTPTFTPTPTFTATATPTP"
     gene            complement(18445..19407)
                     /locus_tag="PLE39_07510"
     CDS             complement(18445..19407)
                     /locus_tag="PLE39_07510"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNE68210.1"
                     /translation="MFKKFKYLLFTLIVVLSLLITACSGSAETPETTQQDTNSIQTAV
                     AETVAALGNVSNPTPEAPAFTPTPLLFAPTFTPLAPVASPTRTRNPSVSECAQASLVS
                     ENIPDGTIFKPGQGFTKTWEIKNTSPCSWDTSYKIIFWDGNVLGGAYVYNLPQAVDPG
                     QIVPISILFTAPQADEAYTSKWMLQTPDGVEFGVGEYSAPFYTQIVVSSSANPGFAIT
                     SVEYNVVRDPATGCPANVNHIVYATFTSNGPIEFTYYWMQSDNNNSNPKKLKMDAAGT
                     LTISREWKLHLGSTPGTKWMAIVIVDPVEQEYPHAEFTYLCGSS"
     gene            complement(19493..20464)
                     /locus_tag="PLE39_07515"
     CDS             complement(19493..20464)
                     /locus_tag="PLE39_07515"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNE68211.1"
                     /translation="MKKQIRLFNLLMIFTLFLSACNLPSNNAEDVASTSAAQTVEALL
                     SATPAIIINNTPTFTVAPPVTTATLTPIPLPVLTNTPIATATSNCNVAEFVADVTIPD
                     GTVVTPNQAFVKKWRIKNIGSCTWTGFSMVFDSGDSMGGPASKPISTVAPGQEIDLEV
                     NLTAPATLGQYKGYWRIVTNGNVLVPVVKGYQGKSFYVDVKVQAATTGSPSVTTTITP
                     TATVTSTPFVFTVTSVLFNVTGSCPNFHYTFSVTTNGPGTVTLHRVFSDGSTDPYPAS
                     LTFNSAGTQTSPSIPMTFNTPGSSAWVDVYIDSPNQKQFGRATFTCP"
     gene            complement(20797..21342)
                     /locus_tag="PLE39_07520"
     CDS             complement(20797..21342)
                     /locus_tag="PLE39_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68212.1"
                     /translation="MKKYIYMLILVVLVVSISACGGGNPAPTEASADSGAPVDSGAPA
                     DSGAPADPGAVVDAPADSTDGGAPDPMDGFMFPPMPGEAEPQDDVNYFVAILREQSGI
                     SGDLQLMSAVPATTTWDTVFAYYDQQAKNNGWVLEKTEEVKTQKGYLCTVALFTHGKN
                     KILIAYYPLNGVVNILQVQGQ"
     gene            complement(21481..23016)
                     /locus_tag="PLE39_07525"
     CDS             complement(21481..23016)
                     /locus_tag="PLE39_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68213.1"
                     /translation="MTEAKIVLEARNITKQFPGVLASDNVNFDLRKGEIHALLGENGA
                     GKSTLMNLIYGLHRPDSGEIAVNGKTIEIDSPNDSIAAGIGMVHQHFMLIPVFTVAEN
                     VMLGDETVKRGSLDRAAVATRINEISKQYGLDVDPNALVGDLPVGLQQRVEIVKTLYR
                     NAEIVILDEPTAVLTPQEAEDLFRIMRDLTARGVSIIFITHKLKEVLAVADRITVMRA
                     GRVINTVQPQGTNEAQLANMMVGRQVILTVEKDDHAASGDILKVEGLSVRDQRNLETV
                     HNVSFTVRGGEVLGIAGVQGNGQTELSEALTGLRSPTGGKVTMDGIDLTGKPPRIMTE
                     AGLAHIPEDRQRHGLVLSYSIADNMALCDYYRPPFSNRGVLQQKALDENARKLIGAFD
                     VRTPSPFVSAGKLSGGNQQKVIVARELSRSNVKLVIANQPTRGLDVGSIEYIHSEIIK
                     MRDRGVAVLLISAELDEIMALSDRIAVMYRGQIVTVVEAKKTTREQLGLWMAGAHVES
                     SPA"
     gene            complement(23026..24138)
                     /locus_tag="PLE39_07530"
     CDS             complement(23026..24138)
                     /locus_tag="PLE39_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE68214.1"
                     /translation="MDWKRFGFIALIIVVLFGTVVMIGQVKQPPVLIITSMLATTIAV
                     ATPLALGALSGVYCERAGVVNIGIEGMMLTSAFFGWLGAIYMYHVFGFGVGFSIGMGV
                     FFAILTGVLMSLLHAVLSITFKVDQIIGGTVINILAVGLTGFLNRQLFFGKGSVFGGS
                     VPNSPGVLPTIHVPLTEIFTSVCGSSASCLENLAAINRVFDQKPIAISAILLVFFSHY
                     VLFNTRWGLRTRAVGEHPKAADTVGINVIKMRYANVLIGGMFAGLAGAYFTIESVPSF
                     EPLLTNGRGFISLAAMIFGNWTPIGAWAASLVFGASQALLINMQIFRDVIPPQWSFLQ
                     ESYIVGLTPYILTMFILTGIIGKTTPPAADGVPYEK"
     gene            complement(24147..25274)
                     /locus_tag="PLE39_07535"
     CDS             complement(24147..25274)
                     /locus_tag="PLE39_07535"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE68215.1"
                     /translation="MQSEKQSNSLIKQILQTISDNSLVPFLAILTAVVVGGVIIAFVG
                     GNPILAYRGLLEGSFGSTKALSETAVWATPYIFAGLAVALAFKGGLFNIGAEGQLAVG
                     AVASSLVGYALPEWLGYDLPAIIHLPLAIVFGVLMGGLWAAIVGWLKAYTGGHEVINT
                     IMMNYIALNTTSYLLNGVMKDRSPTNVIARTPLIADSARIPPIFDGLRVHWGFILALL
                     VAFGIWWLLNKTTLGFQIRTVGLNPDAAKYAGINVKRIVILTMALSGMLAGLAGTIEV
                     TGLNYRHELGFSQGYGYDAIAIALLGKSHPLGVVLAAFLFAAMRNGATRMQFLTQMPV
                     DLISMIQAFILLFVAADAIVRYIYRIKSKGERVVLTRGWGG"
     gene            complement(25415..26461)
                     /locus_tag="PLE39_07540"
     CDS             complement(25415..26461)
                     /locus_tag="PLE39_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE68216.1"
                     /translation="MKKLYFVMALLIVASMVLTACGASAPTAEDCSKEEVFCVGLVTD
                     VGKINDKSFNQSAWEGVQKSQADGVADVVQFIETSDAKDYAKNIAQFGDAGYDVIVTV
                     GFGLGEATAAAAATYPNVRFIGVDQFQAETVAGVSGLNFPEDNAGFLVGALAAMMSES
                     HKIGAVCGTDVVPPVWRFGEGYKAGAAYADEKNGTTTEVFVVYHSDVGFDKTFTDPEW
                     GAQTAKSMMDQGADAVFGCGGLTGNGAITAAAQAGAYAIGVDTDQYLTLPEAAPRMLS
                     SAMKLITPGVADLIKATKEGSIADGNVFGAAGYAPFHDLDSSVPAEVKTMMEEINKGL
                     LDGSIKTNVAPVKP"
     gene            complement(26592..27548)
                     /locus_tag="PLE39_07545"
     CDS             complement(26592..27548)
                     /locus_tag="PLE39_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE68217.1"
                     /translation="MSALTACMQSQDCFSEEVFCAALVTDTRGLEDHGMNQETWAGLQ
                     EAQSGGLSDRVEYIESVDTRDYEKNIVFFAEQGVDVIFTIGPGMDDETLRSADLYPDS
                     VFVGISQPQKEARKNLIPVNFAEDQMGFLAGALAARLSGAGVVGAVCETSDIDAMWRY
                     CEGFNAGVKSVDENIKVLVFYRDDASRDDLFLDEVWGYETGQSLIQRGADVIFAAGGA
                     TGEGALRAAMDGQVLAIGAERDQSAEGYGVVTSVYGSASFEVQEVMRMIQGENLSKAG
                     IGSFGYVPLNEKFSEGLTRELDSLLSALLNGEIQTGVSREKP"
     gene            complement(27599..28879)
                     /locus_tag="PLE39_07550"
     CDS             complement(27599..28879)
                     /locus_tag="PLE39_07550"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE68218.1"
                     /translation="MSSRLNYGKTFLLGFGFFGLSVVWGVYNAFVPIFLSEKFGLSPV
                     VIGFFMTLDNIAALFIQPPVGAWSDRLRTPLGRRIPFLLIGAPITAVAFGLIPLPAIL
                     PLFVACTSTLLLSAAFWRTPVIALMPDITPSEFRSQANGIINFMGGIGTIVALQTGGL
                     LYKLSPNFPFWLGSGLVLLSALIVYLFIEEPKEYAASEEQPGMLASLREVVSSEDKSG
                     LRILFAIFFWFIGFSAVETFFTLYAKNHLGLNEGDGATLLSVLPLFFVLFAIPSGFIA
                     GRLGRRTTISIGLITLTIMLVLLYITPAKALLTGISPLPLVGIPLEEGGPRMLTLAGV
                     LLIFGGAAWALININSLPMIVDLTDAARIGTFTGLYYLFSTFSAIVGPNVNGWAIQLT
                     NGNYNIIMLIAPFFMMIALWLMLGVKRGEAAQAV"
     assembly_gap    28922..29021
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29022..29775)
                     /gene="trmD"
                     /locus_tag="PLE39_07555"
     CDS             complement(<29022..29775)
                     /gene="trmD"
                     /locus_tag="PLE39_07555"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNE68219.1"
                     /translation="MQFEVFTLLPEVFSSYLDTSILKRARERGLIDVRVHNIRDYTHD
                     KHHVTDDTPYGGGGGMVMKPEPVFEAVESVLGISNENDSSPIPVILLTPQGRLFNQAV
                     AQELSAHPHIALICGRYEGFDERIREHLVTDEISIGDFVLTGGELPALMLIDAVSRLL
                     PDVLGDPTGAQDDSHAMGLLEYPHYTRPPEFRGWRTPDILLSGDHAKIDKWRREQALE
                     RTFKKRPDMLEKAELTKEDLKFIEKLKNLKPST"
     gene            29875..30576
                     /locus_tag="PLE39_07560"
     CDS             29875..30576
                     /locus_tag="PLE39_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNE68220.1"
                     /translation="MNNYIALVITFAASLLFLRSMDFIAHRGWMDGKLSRKIIHIGTG
                     PLFVLCWFFFNDAPEARWLAALVPFAVTAQFALIGLGVIKDEASVQAMSRTGDPKEIL
                     RGPLYYGIMFVVLTLVYWRENPTGIIALMMMCGGDGIADIVGRRIASPKLPWSREKSI
                     AGMVSVFAGGWLMAGIMIYIYVAAGVFSAPFAQYTLPITLIALAGAVVESLHYKDIDN
                     VTMTLASALIGGLFF"
     gene            complement(30895..31641)
                     /locus_tag="PLE39_07565"
     CDS             complement(30895..31641)
                     /locus_tag="PLE39_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNE68221.1"
                     /translation="MSNEQEYLPKIAMSIQAHPDDQDFTVAGTLAKWAKAGCKIISVL
                     ITSGDSGSNDVTKGANYKSVLAELREGEQLAANAVLGISETVFLHYPDGELTASIALR
                     KDLTRLIRIYKPDVVVSGDPSAWFYGSGYINHPDHRAAAEAAAYAVFPSAGTRLIFTD
                     LLEAGYEPHNVKRLYIHGSEKPDTWVDISETIDIKIEALKKHVSQSDTHDVDDWMHEW
                     AKEEGKEKGLEFAESYKVMILKKEDDIPGE"
     gene            32311..33285
                     /locus_tag="PLE39_07570"
     CDS             32311..33285
                     /locus_tag="PLE39_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNE68222.1"
                     /translation="MKTVQPIQSFDQIKLLADARRMEILRLLMAAPATLTILAQTMKQ
                     SPAWVRHHILALESAELIEVSELRKTGKVTEKFYRAKAAAFLLQEVVLPKSKKQSIIF
                     SGSHDLALEEIAEHLNKHVTLLSMPVGSLDGLVNLRQGLCQISGAHILDATGEYNTPT
                     VRHLFPDRNVELVTLAYRTQGLIVAPGNPRNIKKISDLARENVKFINRNSGSGTRIWF
                     DNELKKLKLDPTQIPGYDQSVKTHSEAAQAVFTKTADVALGLQAAAKQHDLDFIPLFE
                     ERYDLILPRENESLLSPLLDYLQTSTFRSSLGRLVGYNPTHSGEQIAV"
BASE COUNT         8165 a         8873 c         8317 g         7835 t
ORIGIN      
        1 tgaccaaacg cattttcatc tccgccgatc acggcatggc aatcatctac ttcctgcaaa
       61 gcgatgtcgt ttcgaccctg cttgaagcgg gcgtcgaagt catcgtcctc accgacgacg
      121 acaccaaaga aaagatcgcc tcacgttttg ctcgtcaggg tctcaccttc gagggcttgc
      181 gcctcaaaca agccaacgac tacgccaaaa aagttcaacc aagaatgcaa tggctgctcg
      241 gctacctgcg ccgtgtgggc ggttcgcgcc gcatcaacac cgaagccatg gacagccaca
      301 tttgggaggt ctggggcgag aacggctgga aattccgcct cggcatttgg attccctccg
      361 cgctgatgat tttgctgctg cggaattttt catgggcgcg cagcctttta gtgcggatgc
      421 aaaaccgctt cacccctacc ccgggcctgt acactgacct ctttgaaaaa taccaacccg
      481 acatggtgat cgcctccacc cccggctggc gcatggaccg ttatctcctg cgcgaatccg
      541 cccgccgcgg aatcccgaac atgaccgtca tcgtcggctg ggacaattca tccagctaca
      601 acgtcagcgg cgcagatgtg cagtgggcca cgtgttggtc tgagttgcaa aaaaatgaac
      661 tcgtcaaagg ctccgactgg gaccccgagc atgtgaatat cggcggcatt ccatcgtacg
      721 acggatattt ccgcaagcaa tggctcatgc cgcgtgacga atatttcaaa ctgcacgggc
      781 ttgacccaaa ccgcaagttg atttcctacg ccagcagctt tgtccatttt gcgccgaact
      841 tcccgaatat cgaagcattg gcaaaactgg tttcatctga ttcactcgca gaaccttcgc
      901 agttattgat ccgtctgcac ccgagtcact ttcaagacaa gcccaaaatt tttgcagacg
      961 aacgcgcaca ggtttttgag ttggagaaga aatacccgca tgtgcatgtc gtccagcctg
     1021 tggcgttggg tgggtcgctc ggctattacg gcggcgaaga catggacgaa aaatcttcga
     1081 tgatggcgta ttcggatgtg gtggtgacgg tctactccac catgctggtc gagaccgccg
     1141 tccacgatac gcccatgatc gccgccacca tcgacacccc aggcggttgg aacaaaccga
     1201 acaagttctc gctctcattg aaagagatcg gcgactggcc tacgcatcaa cgtttccgct
     1261 gggcgaaggc agggcgcgtg gcagaaaacg aggagcaact tcgggacgcg ttaaatcttt
     1321 atttgacaga ttcaactgtc gacgcggccc agaggcggaa attcgtcgaa gatgagatca
     1381 catttacaga cgcaacttct ggaaagcgga ccgcagaatt catcctgaaa gtattgggca
     1441 aataaaaata acggcggcaa agaaaaaatc tttgccgccg ttggcgtgct tcgcggttta
     1501 aaactctttg tggtaaaaat gggggctgga gaaacaaatg aacaagaaac aactgaccat
     1561 cctttccacc ctcatcctct tcatcctatc tgcctgcgga ggggccgctg agcccaccat
     1621 caccgcagcg gacgtggagg gcacggccgt agccaatgca tggatcgcgc ttacccaaac
     1681 acaagctgca ctccccaccc agaccccctc cccaattcca agtgaaacac ccacgatcca
     1741 acccaccatg acggcagccg cattggttct gcccacgctg gctcccccca ctctggcagt
     1801 ggtcaccacc cccaccaacc cagcggatga tccctgcaac cagccgccgc cagccgaacc
     1861 caaagggctc acggtcaagg tcaagttcgt caataaatct ggcggacaag ctgcgctgtc
     1921 atttggcatg aactttccga acaagatcgg cgaatgcggc acctacggct acacccttgg
     1981 gccattcgat gaaccaaccg tcaccgtatt ggcaggttgt tattggggct atgcctggat
     2041 cactggcgac aagccctcgg tagccaagac aggcgatgcc attctgtgcg ttcaagaccc
     2101 caacgaagtc cgcgccattt ggatcacggc tgaaaacatc ggcttccatt agcccaaaac
     2161 acctcgacca ttctcaacgg gaagggctga ccaaaaccag cacttcccga tagaaacgtc
     2221 ccgaaggcca gcaagaacct tcgggacgat gcgtatcatc cctttactca ttatgaaacc
     2281 aaaaaccatg tccctctttt atttctcgat cactctggcc atttgttcca gcgcactgta
     2341 tcactttgtt gccaaaagca cgccttcggg tgtgaatttc accgtttcac ttttggtgac
     2401 ctatgccgtc gctttcgtgg ttacgctgtt caccttcttt ttcttcccaa ccaaaaacgg
     2461 gatcgccgcc gagttgaaac agcttaactg ggccagcatt gggctggcaa tcgccatcgt
     2521 cggcattgag ttcggattcc tgctcaccta ccgtgcaggc tggcatttgg gcattgccgc
     2581 cgtattggtc aatgtggtgg catcgctcat ccttgtccct gtggcgctcc ttgttttcaa
     2641 ggatgaaatc tcgtgggtga acattctcgg gattctgatt tgtttggtcg ggctggttat
     2701 gctaaactgg aagagatgaa acatacaaag ttccttctta tccttctcac agcgcttctc
     2761 atctcatgca ctccaacacc tgccccggta cactttccca cattcctacc caccgccact
     2821 gccaacgccg cttctgtacc gtccagtgtt tccactgcca cgctccctgc cgctgcaacg
     2881 gttgaaacct tttacaaatt catcaatgcc gcccaatctc ccaatgactt caacgctgcg
     2941 tgggatatgc tcaccaacga ggagcaatgc aatacacctg aaaaatgcga actgagttat
     3001 tttcaggaaa aatggatgga atcgaaggtc atctacaaaa tctacgaatg cggccccgat
     3061 tatgccatcg tggaggaacg tctttatccc cgaactgccg atcccgcctc ggcaagttct
     3121 gaggcaaggt tgatccgctt tcaattggca caggccgatg aacaaatctt catcacgaat
     3181 agaaaaacgg tcatggcgct ggaggcgggc tgcgttctga tcgttgacag cgccctcagt
     3241 ccgtaacctt gcccttttcc gttacaatag gcgggctgtg tcagaaatga aagctccctt
     3301 tttctcctcc cgcttcgctc aaattgccgc gtttgttctg ctgctgggtc ttgggcttgg
     3361 aatccgcctt tacgacctca ccgacctccc actcgatttt catcccacgc gccaattgct
     3421 ctccgcgctc aaagcgcgcg gcatgtatta ccaaaccctc agcgatattc ccacagaaca
     3481 aaaaagcttc gccgtacagc aatggaagat ccgcgcggcg atcgaacctg agatcgtcga
     3541 acgcatcgtc gcattcacgt atcaattcac gggcgagcag atctgggttg caaaagtttt
     3601 ctcctccgcg ttttgggtga tcggcggact ctttttgttt ttgctcgcac gtcaactcac
     3661 ctccaccgaa ggcgcactgg ccgccacagc cttttatctt tttacgccct acgccgtcgc
     3721 cgccagccgc agttttcagc ccgacccatt gatggtgatg ctcattatca ttttttggtg
     3781 ggcggtttac aaatggagcg aagacctcac ttcttttcaa tgggcggtca ttgcaggtct
     3841 cttcggcggg ctggccattt atgtgaaatt cgtcgcggca ttttttgtca tcggcggcgg
     3901 gttgggatcc attctcagac gagagtctct ttttgaaaca gcacgcaggc cgcagattta
     3961 tgtcatgacc gttttgggca tcctgccagg cgcggcgtat gtcatctatg gggtctggat
     4021 cgcgggttac ctcggtcagc aattcggcgg gcgattcatt ccagcgcttt tcctaagccc
     4081 atcctattac ctcggctggg tcgggatgct caacatcgtc atcggtggaa tcgctttgat
     4141 gctgggattg ctcggtttgt tttttgtgga tgaaaaaaaa cgccgcttct ttcttggact
     4201 ttggggcggg tacgccctct tcggcatgta cttcaactat cacatttcct cgcacgacta
     4261 ttacagcctg ccgttgattc caatcgtggc cctttcactc gcgccactgg cagaatggtt
     4321 cttcgctcaa ctcacagcat tgacgccaac acaccttgcc cgtttttctg ctttcggcct
     4381 tttgctgttc ggaattttcg cctccctatg gaatatccgc gccgaaatga aagcgaccga
     4441 ctaccgcccc gaagcccaaa tgtgggcgga gatcggcgag accctcgaag ataaaaacac
     4501 agcaggcctc acgcaagatt acggctcacg cctcgcctat tggggttgga ccagcatcac
     4561 cacctggccc acctacggcg accttatcta tcatgatgac ctgcgcggcg ctcaactcga
     4621 attcgacggg ctgttcgaag acattgccct caaaaaagaa ttatttctgg taaccgattt
     4681 cgatgaatta aaacgccagc cattactaaa agaaaaattg tctgggtatc ccatctttgc
     4741 cgaaggcgat ggatacatca tcttcgatct cacctctaca ggttcacaat aaatgaccaa
     4801 aacaaccaaa ccgactttcg atttcatcac cattgccacg ggcctgggcg cggccttctc
     4861 ggcttttgcg ctcggggtgt atgcctacct cggcattttc tcgcgccatc tcgccgatga
     4921 ttattgcagt gtagacttca tccgcaccaa ttttttcagc gcactgctga ataactacat
     4981 ttttgtttcc gaccgctaca ccaacttcat gctcattgca ttgagtgaat acatttcacc
     5041 gcgcacggtt tcgatcctgc ctgccctgtt catcgtcctc tggttgacag gcatcgcgtg
     5101 gatgttatct gaagccagcc gctttaccaa aaatggctgg tcgaaactca ccattgtttc
     5161 gcttactttt ttggtggtgt tcttttccct gctgcaggcg cccaaccgtt ttcaaatttt
     5221 gtattggcgt tctgccatgt ccacgcattt tgcgccgctg gtcttcatgc cgttcctagc
     5281 ggctttcatt ttgaaggtca tcaccgaatc tgaggggaaa cccgccccat tttggaaata
     5341 ccttctcggc ttctttattg ccctcatcat cggcggcttc tccgaaccga ccgtggcagt
     5401 gatgatcacc ctgctggccc tcgccttcgc cgccacctgg aaatggatgt caccatccac
     5461 ccgcagtgcc gccctgaaaa tgatcggttg gtcactggct ggtgcagtga cggctttgct
     5521 catcatgggg ctggctcctg ccaattatta ccgcgtcagc ctcggcaagg aaccaccgac
     5581 gcttgccttc ctcattgtcc gctcttttca gtatgggttc aacttcattg ataactcctt
     5641 caggaccctg cccctgccca ccctcttcac cgtggccatg ccgttcttca tgttctacaa
     5701 ccgctacgcc tcccccaccc cagccctgac cgagtcccag cgcaaactgg ctcgcaccct
     5761 gttgatggtc atacccgcgc tttcgtattt gctcatcgta gccagttttg ctcccagcgt
     5821 gtatgggcaa tcctttcccg tggagcgggc gcggtttgcg ggccaggtca gtttggtggc
     5881 ggggttaatg atcgaaggcg ccttgcttgg aagtttactc gcgcaatggc ggcctgcctt
     5941 cgccgaaaaa cttccgctta aactggcatc ggctgttctg tttgcagtgg cggcgcttta
     6001 tcccttgcgc gctggctggc tctccctcaa tgacatcccc gattaccgcg cccgtgccga
     6061 agcctgggat acgcgtgtga ttcagatcaa tgacctcatc gcccagggtc aaaccgacct
     6121 gctcatcgtt caactcgatg gcgtggaagg tgtaaaagaa ttggaagtca ccgagaagca
     6181 ttgggtgaac cgctgtgctg caaaatatta cgaagtcaac tccatccgca ccgcaccgcc
     6241 aaaatgagca tgaaacgacc gatccttgtc acaggggcgc accgcagtgg aaccacctgg
     6301 gttggccgca tgttggcggc gaacctgcag accgcttaca tcagcgagcc gctcaacgtg
     6361 ctgcatcgcc gcggcgtgta tcgcgccgag gttcaacact ggtatcaata catcaccagc
     6421 gaaaatgaag gcgcgtacct gcctgccttc catgaactgc tgaactatcg ttatcacctt
     6481 tttgcagaga tccgctcgat ccgctcgcaa aaggatttcc tgcgcatggg ccgcgacctt
     6541 tccattttta tgaaaggcaa attacagaac cagcacgccc tgctcaaaga cccgttcgcg
     6601 gttttctccg cgccgtggtt cgcaaagaca ttgaattgcc aggtcgtcat cactgtgcgg
     6661 catcctgcag gctttgccag cagcctcaaa cgcctcaact ggcccttcga tttccgcgac
     6721 ctgctcaacc agccaatgct gatgcgtgac cacctcgaag aagaccgtgc cgaaatggaa
     6781 tcaaccccag cggatgacat catcggacaa tcgtccctgc tttggcggat gatctaccgc
     6841 gcggttcacg cttcgcgtac ccgcggcctg atccccgaaa tcaaaattgt ccgccacgaa
     6901 gacctttcac ttgaccccat agcagggtat aaatccctgt atgagtcgct cggcctgacc
     6961 tttgacgaaa acgtgcgcga taccatcctc aattccagca gttctgaaaa cccaaccgaa
     7021 ctctcgaaga agaaggtcca ctcggtgaaa ctggacagcc gcgccaacat ggataactgg
     7081 aaaaaacgcc tgaccgccga agagattctc cgaatccgca agatgaccga aagcgtctcc
     7141 cacctctttt attcagaaaa tgaatggtga cccagtcacc aacgaccaaa ttaccaatgt
     7201 ccaccctccc tctcgtttcc atcatcacac cctcctttaa tcaggcgcaa ttcctcgaag
     7261 ccacgattca atctgtgctt gggcaggaat atccacggat cgaatacatc gttgtagatg
     7321 gcggctccaa agacggcagc gtggatgtga tccagaaata cgcgggtaga ctcgcctggt
     7381 gggtcagcga acaggacaag ggtcagaccg atgcgatcaa caagggtttc aaccgtgcca
     7441 cgggcgatat cctcgcatgg atcaactctg acgacaccta caatccgcag gcggttgggc
     7501 aggctgtaaa acagcttatg gaaaatccag aagtgggact ggtgtacgct gactgtaact
     7561 acattgatga agcagggaat gtgatcggca aattccctgc cgcgcaaacg gatcaccgcc
     7621 gcctgcgtga aggttacgtc cacattccgc agcagacaat gttcttccgc gcaaagtatt
     7681 ggaaggaact cggcccgctc gacccgtctt tttattttgc aatggattat gatctttgga
     7741 cacgaattgc aaagcatgcg ccgttcaaat acatcgcagg gcaaacgtgg gcgaactttc
     7801 gaatccacaa cctcggcaag accacctctg ccgatgaccg ctgctggcct gaaatgatcc
     7861 gcgtgcatta ccgcgatggc ggcagttttt tttcggtgat cgtggcaaag cattatctgc
     7921 gcaaagtcat tggcccgctg tggaaatggc ggatcaaaaa gcaagcgaac cgttaagcga
     7981 gtttctttca ctcttcccat gacacatctt ctcaccccac ccactcttga agacctcatc
     8041 cgcctgctta aggcatggcg cttttggatg ttcggcgcgc ttgttggcgc gctcatcggc
     8101 gctgcggttt attatgcaga tccgccgccg tttcgtgcgc aggccaccgt caatgttgat
     8161 ttcaatctgg aacaagcctg gccgcaggaa acggaccgtc agcagtttta ttatctcgag
     8221 cgcgagtcac gcaaattgga agaagtcgcc tggtctgatg aagtgttggg gcagctatct
     8281 tcttcattcg gcatttctgt ggaagaactg cgcaggggta aactgcaact cagccagcca
     8341 gctgaagcgg gctggcattt ctacgccgac gatgacgatg cacagtttgc cgcatcactg
     8401 gcgaccacct gggcgaaggc cttcgtagcc aaaaccgaag cagacgtggc ctcaggcgcg
     8461 atcaacgaat tcgtccgcct tgaagcgacc caatcggaaa gtactgccaa agagcgcagt
     8521 attccattga gcagttattt actggcgggt tccatcggtt ttcttacact cgctgtgttc
     8581 gtattgttgt ttaaacgcaa gtgaacgcgg attcagagat gagaaaaaag atttttatca
     8641 gcattcatct gcgtcccaat taatttttta tgaaagaatt tttccccaac tcttacaaaa
     8701 tctcccgcat tctgtggggt gcggctttgt tgaccctccc cgtgaccagt ttccgctggt
     8761 ttcctttttt gggagaaggc accctggtcc gtccactggc gatgtacccg ctggcggttt
     8821 tgattccgct cttgttgatt ctggcatggc gtggaaaaac aaagctcaac tggtcaggcg
     8881 cgttgattcc gcttggggtg ctggtcctgt tcatggtggc agcgaccagt ttcggggcgt
     8941 tgatcgatcc gatcccgttg cgcgggcaaa cctattcggg cagagccatc cgcgcgctgg
     9001 cgactctctt catcggcatc gcctttttca tcgctgccat gtggatgaac aaagatgaag
     9061 atgatttccg tttcacggtc acgtggctgt ttgccgggtt gtgcctcgac cttgcatgga
     9121 gcggtttgca ggcggtcacg ttttatacgg gcctgctcga aaaagaaatg gtgacgcatt
     9181 ggcagcgcgc attttcaatg cgtgaactcg tgcgcacaaa tcgaatctcc gggcttgcct
     9241 acgaacccgc ctggctggca ggacaattcg ccacaacctt catcccgttc ctgttcgctg
     9301 ccgtgctgac gaatttccgc atcacacgcc tgaagtggct ggaacctgtg ctattggcat
     9361 tgtctttgct cgtggttttg gcaacctact cacgcggcgg gttgctcacc acgcttgccg
     9421 cggctgggtt gaccttcctt tttacggggc agcttggcat cgtgtggaag tggttcattg
     9481 gcggcttccg cggggggatg cgcggaattt tattccgtgt cggcattctg gccgcaattt
     9541 tcagcgtggc ggcgggtgcg ttcatttttc tcagccagaa gaatttcttc cgccgtttat
     9601 gggagacgga tgcgcaaagc ctgagcgaat acattgtgga tatcaatgcg ggcgcgcgcg
     9661 gcgcctattc ggctggggca ttatccgctt ttgaggagta tccctgggcg ggtgtggggc
     9721 tgggggcaag cggtttttac atttaccaga acctgcccga ttggtcgctg accaccgtgc
     9781 cagaaattgc caaacaactc agccctgaaa acaagttata ccccaaccct aaaaatctct
     9841 atgcccgcct gttggcagaa acaggattga tcggcttctt tttattcctg gcatttcagt
     9901 tttacatact tggcgatata ctcagccttc tgcgtcacag ggaaggctgg gcacgttttg
     9961 cagcagtggc gggaatattt gcatggttgg caatcacatt ttataatttc acacaggatt
    10021 cgctcacaac gccaaacatc tgggtcattc cgggcatact ggtggggctt gcagcgtctt
    10081 ccacaatgcc agaagaaccc ctgcctggca ttggactcca atctaaggaa aataaatgat
    10141 cgtattatct gtcggtatgc cgcgggctgg gtcgggctgg cactacaact taattcacga
    10201 ccttatgaaa accacaggct gtgccgatgc gcgtgatatc cgcgaacggt atcggcttca
    10261 aaatgtcttg accgaggtca attgcaacat cggtgtgctt tcagccagaa gacttggact
    10321 ggtgatgatc cctgcgctgg taaatacttt tgtcatcaag gctcattccg cgccgacgaa
    10381 tgcttcgcgt ctgctttcga ccatgggcag gctgcgcatc acgtacattt atcgtgaccc
    10441 gcgcgatgcc atgctctccg cttatgattt cggtcagcgg gcgcttacca agggccgccc
    10501 caatgccttc tcgcatcttt caagcttcga gaagagcgtg gattttatga tggaatatgt
    10561 ccatatttgg gaaaaatgga ttaaagaaaa gaacgtgctc accgcacgct acgaagacct
    10621 gctgatgaat tacgatgagg aatcaaccag actggtggaa tacctcaaat tgaacggcag
    10681 caggcctgag gtccgcaagg tgatcgagca atatcgtccg ggcacagcgg aaggtcagca
    10741 gggcttgcat ttttataagg gcaagatcgg ccgcttccgt gatgcgtaca acgaggaaca
    10801 aaaatccatc ttaaaagaga aacttggccc atatttgact caaatgggct acaatgaatg
    10861 acaaggtgta atctatttgt aagtcaccca agttgtcgcg cggaatgttt ttcgttatca
    10921 tagggaagtt atgttacgaa gatttgattc gccctacatg gcagactggt ttgccatatc
    10981 attgagatgg atcattctgg tcggctttgt tgtttcactt ggattggggg acaaacttga
    11041 aattgcagtt tcgtggccgc tggggttatt gattctctgg aacctgttca tgactgcact
    11101 ggcaagcatg aacatccgtg ttgcctatca ccgtctcttc agcattcttg tcgatatcac
    11161 actggctggg ttgttctttt ggacccaggg cggcctgcgc ggacctgccg catgggcggg
    11221 cattttaccc attctgacgg ggtcgatatt ttttgagttg ttgggcgccg tagccgcctc
    11281 tgtcttcttc tccgggctca tgatctattt ggggctgcaa gacggggaca gtctgccgct
    11341 ttccatcaca ttctcagctg cactgatcgc attaagcatg gtgtttggtt ttctcggcaa
    11401 acgcatgatc attcacatgc gcaagaaccg cgaactgtgg ctggattcag aagaacgaaa
    11461 aagcgccatg caagccgagc ggatgcgcgc catttacgaa ttaaccgccg cattatcctc
    11521 caccctgagc tataaacgcg tgctcgaatc cgcgctgaat ttgagcgcct cggcgctaaa
    11581 ccccgacccc gaacaacttc acagcgatcc gcttgtcggt gtggtgatgc tgttcaatgg
    11641 cggcaaactg cgcatcgggt cggcacggcg cttcaccacc gccgacatgc gtgtgacctt
    11701 cgatgcggcg gaaggcatcc tcaaaaagac aattgacgaa ggtgaaccgc tctggttcaa
    11761 agacattggc tacgaccccg aactgggccg tgtgatcgcg ctccgcaatt gcaccagcgg
    11821 atattgcttc ccgctccgca gcggcttcaa cgtttatggc gttctgctgt ttgcccaccc
    11881 cgatgcagag tacttcacca ccgagcgccg caacctgctc gatatcatcg gccgtcaggc
    11941 cgtcatcgcc attcaaaatg cgcgtttgta tcaggacctt gttgaagaaa aagaacgcat
    12001 ggtggagatc catgaagaag cacgcaaaaa actggcgcga gacttgcacg acggccccac
    12061 ccaatctgta gcggctattg ccatgcgtct caacatcctg cgcagaatga tggcaaaaga
    12121 cgtcgcatcc gccggggacg aagtggtcaa actggaagaa cttgcccacc gcaccaccaa
    12181 ggaaatccgc cacatgcttt tcaccttgcg cccgctcatc ctcgagtcgc agggattggt
    12241 cgccgccatt caagccatgg ccgataaaat ggctgaaacc tattcacaaa aagtgaccca
    12301 caacattgac gaacgggtca cctcgcaatt ggaaatgggc aaacaaggcg tcatcttcta
    12361 cattattgaa gaagccgtga acaatgcccg caaacacgca gcggcagaga ccatcgcggt
    12421 aaacctcaca caaatggatg caggcattgc cctgcttgaa atcgccgaca acggcctggg
    12481 cttcgacgta aaatcggtca ctgaagcata cgacaaacgc gcaaacagca gcctcggcat
    12541 ggtaaacctg cgcgaacgcg cagaactcgt gaatggcttg ttccagatcg actccgcgcc
    12601 cgggaagggt acgaaggtac aggtgtacat cccgctcaca gaggaagccg cagaccgttt
    12661 acattacatg cgcaacaaat aaaaaatgcc catcgcagca aacctttatt atttttccca
    12721 cgacacagaa gacgacctcg aaaccaaacg cccgcccatc attctcattc atggggcggg
    12781 cggaaatctt ttgtcctggc cgccacaggt ccgccgcatg gtggatgaaa gggtgtactc
    12841 actcgacctg cccggtcatg gcaaatcgga aggcacaggc aggcaatcca ttgacgagta
    12901 cgttgaagat gtactggcat ttatgcaggc cttgaaactt cgcacggcca tcttcgcagg
    12961 cgtttccatg ggtgcagccg ttgccctcgc actcacacta aaacacccac gcaaagtgcg
    13021 cggactttgc ctgctgggca gcggctccaa aatgcgtgtg gctcccctta ttttggaaac
    13081 agcgggcaac cccaacacct tcgaatctgc tgtggatttg atcaatgaaa actgcttcag
    13141 ttccgaagcc ccgcaacacc tcaaggatat ttcaaaacgc cacctgcttg atgtgcgccc
    13201 gcccgttttg ctcggcgatt tcctcgcctg tcatgaattc gatgtcacca gccagctcaa
    13261 aaaaatccgc acccccaccc tgctcatttg cggcgccgca gacaaaatga cccccgtccg
    13321 ctattccgaa ttgatgcagg aaacaatgcc caacgcaaaa cttcacatcg tggagaatgc
    13381 agggcacctc gtcatgagtg aacaacccga tgtcgtagca gacctgatga aacagttcgt
    13441 cgattcaatt ccggtccgca ccaggaagac gaaaaaagac cgcgtcgaac ttaacgttga
    13501 atctgatcct gcccaggaaa cagaatcagc acttccggca gattctgtcc cttcgacgga
    13561 gcccaacagc taagaaaata tttccttcaa aacaggcatt gcattaataa ccgccctggc
    13621 ccgatggctg aggcggtttt tctcatccat ctccagttca gacatggttt tgcccaattc
    13681 aggcatgaag aaaatgggat catacccaaa accgcctgtg ccgcgttcct cagggatgat
    13741 ttcgccgtgg caattgccgt cagcgatctt cacatcgccg cctggaatgg caatggcaat
    13801 cgtggcatgg aagtgtgcct tccacggacg cggtttacct tgcaggtttt cgagcatgta
    13861 ggctcggcgg tcagcgtcat ttgcgcctgg cttggggtgg taacgggcag aatacaaccc
    13921 gggtgcgccg tcgagcgcat ccacttccag gcctgagtca tcggcaaggg agatcaatcc
    13981 gctggcctgg gcgaaggcga tggctttttt ggctgcgttt tcaatgtagg ttgccccatc
    14041 ttcctccaca tcgagatcca ggttgatctc agcaggggtg acgagttgga cattcaattc
    14101 tctgagaagt tcctgaagtt cttttacttt gcctttgttg ttggtggcga taagaagttt
    14161 tttcattttg aattcctgtg gcgcaagata atatcttgcg ttacatgttt ctttgcccat
    14221 tgtgcatgtt cgcggatcat gggttcttca tcttgagcgg cttgttctaa aattggaagg
    14281 tcgttttcac tgcccgtatt gccaacagca accgctacat tgcggagata cccacgtcgc
    14341 ttggcacgtt tgactgggct tttcttgaag cgctgattaa attcaacgga tgtcagcagc
    14401 aggtctgagg tcaagacagg aagcgggatt ttcgattcga aggcggggtc tgcctgaggt
    14461 gaaaaccgat tccatgggca ggcttgttga caggcgtcac atccgaaaat ccaattctgc
    14521 atgagtggac gtaactcttc ggggatgtcc tctttgagtt cgatggtgag gtaggagata
    14581 cagcggcttg catcgagggt acggttgggg aggatgcatt gtgtggggca ggcatcgagg
    14641 cagcgtgtgc aggtaccgca atggtcggtg atgagggcat cgtcgggttc cagttcgata
    14701 ccaagcagga tctcggcaag gaaaaatgca gagcccatct gcgggttgat gagcatggag
    14761 ttttttccga tccagccaag gcctgcgcgt tgggcaagtt cacgttcgag gatgggacct
    14821 gtatcggtgt aataacgatt tggaatggga tgcccgagtt gttcttcgat gaaatccacg
    14881 atctgctgca ggcgcagagg gatgatatcg tgatagtcat cgccgagagc ataggaggaa
    14941 atgcgaaacg cttcgcgtga agattgaggg ttgaaggtta caggttggta tggcagggca
    15001 aggacgagaa tggatcggca ctcggggaga atttgtttgg ggtcggcacg gcgaatgcgg
    15061 ctgcgttctt cggcaaggta ttgcatgttg ccgtgtctgt tttgatgaag ccattcttcg
    15121 aacatgccgt agtgtgcagg cggctcgcaa gaagtaacgc cagccaggat gaatcccagc
    15181 tggcgcgctt tgtctttgat ggcttgcttg aggtcggaca agttaattcg ggaaaggtgc
    15241 gtcgcactca atgtagaatt gctatttgac gacgatcttc acaaagatgg cttttccaaa
    15301 cgtaacgccc ttggcatttg ccatctgcca tgcactggtg tattcaccga tctggtcggg
    15361 agccttgaag ttgacggata cttccacttc ctgccccact tcaacaaggc caatgggaac
    15421 gggtacgccg ctcatcttgt caccataggt gtaaaccaag ccgtaaccat ctccccaggc
    15481 acagatccct gtatttttga tcttccatgt tttgacaaat tcctgtccgg gggtcatggc
    15541 ggtgccatcc aggatggtga catccacagt agcgacgtca aatgaatagt cgtcgcataa
    15601 tggccctcca ggagtaccca atgcgatctg ggttggatct gttgcaaatg caatggttgg
    15661 ggttaatgtg ggcggttcag cagtgaaggt gggggtggga ggcattggtg tgggtgtgaa
    15721 cgcagcagcc gtcagtgtaa attcggcgac tacggtcatg gcggcagaag tgcggacggc
    15781 cagcacatcg ggcgtcggca cgatgggggt ggcaggggca caggcggcca gcatcagggt
    15841 ggcaattatt gcagaaatga caaatctagc tttcatggtt cctctcttga tgtataagaa
    15901 caattacttg atcacgtact taacccagac catggagccg aagtaattgc cctcatcatc
    15961 tctaagtttc caagtaccaa tgtattcacc aggggcgtta tttgtcacca gcttcaagac
    16021 aaacgaaata ttggcgcccg gtttcacata atcttccgct ttcttgatga caatatcgga
    16081 gcctttaaag cccggagtag aaaaatcagg aaggaaggca aaggtatagc cttcgtccca
    16141 cacacaagaa ccggtgttca aaaattggaa gaccttttca aaagccttgc cgcgctgcaa
    16201 tacatcacca tcgaaaggct cgctctcctt gaccaaaacc ccgtcattgc agccattctt
    16261 ggtggtgatg gtggcaaaaa cagcggctgt tgggatgggc gaggccagcg gggtaatacc
    16321 cagaacaggc tgctgagtgt tgaagggaat tggagtggca tcaccaccgc cgacgggcgc
    16381 aaatgtgggc aaagccgcaa gagtactagt tggcagaagg gtattggtcg gcaaaggagt
    16441 tggggaactt tgtgccgcca cctgggtcaa aacagcgtta aaggcttctg tctgaactgc
    16501 tgccagatcc tgtgttggga ccggggtagc gcccagatta caggaactta acaccatggc
    16561 aagcaccaaa aaagacgcca gccaaagaaa tttacgaact ctcattatag taacctccgg
    16621 acatagttaa ctcaaagttc tgcccaaccc cacgattttt gaagattttg aagcaaaatt
    16681 gcgaattcgg cgaggcagaa actttgattc gttaacttga ttataacgca cctatgaaag
    16741 cgtttcttat ggagtatcgc ataaattggg catgcagatt ttccgttttt cacaaaaagg
    16801 gagaaaattc cagtagacct cttgcaaaaa tctgaaagat taaccactga gccacggaga
    16861 cacagagaaa aattaaaaac tccgtccgca ggaggctccg cactgcctca gtgtcttcgt
    16921 ggttcaaaat ggcatttcaa agagatgcct tacagcttat gcaaaggatc aagttaacaa
    16981 tttttgttgc tgacccagaa gcgggaataa aacctgcttc tgggtcagca accgatacgt
    17041 tacaaccaaa caggagaaac tcaaattatc aaggtgtggg agtggcggtc gcagtaaagg
    17101 ttggcgttgg agtgaaggta ggtgtaaatg tcagtgtagg gatggttgtg ggaaccggtg
    17161 taccctgccg caaaatgaag ggatgcagcc agatggcatc gtcctgattc tgtccgccgt
    17221 gagcggtgac tacaaggatg aacttcactg tttcaccggc cagagggctc aggtcaaaat
    17281 cgatcgggta ggaattccct tcatacactt cattccaggt aaacaggttt ttcactgtgc
    17341 cgttatttcg gtaatcaaga cgaaagacca catcgcaccg cctcgaacca tactggcaac
    17401 ccacaatcgt ccgaaaatga tcgccttcct tcacggtaaa ggcaggatac tgtccactaa
    17461 tttcaccctg acgcgcatcc tgtggaacag tgagcaggct ggggttattc tcggttacgc
    17521 cgttttccat catgggggta tccaacctga caacaaaacc atttgcatca cccctcgtac
    17581 ccgggcacgg cagaggagcc ccctcgttgc gccactcggc ttcacaaaaa tgctccacaa
    17641 agctataagc catgtagctg gcgccaggca ccctgatctc aacccagaat gccttttggg
    17701 ctttgtcgcc aatcccaaaa ttaacaccat tggcattgcg taatttccaa tagccaatgt
    17761 attccccttc cgcgctgggg gcggtcaact gaacagagat gtcaattgtg ctgccagggc
    17821 ttacactctt cgaaagagct gcggtggttg ggccgctcat tgcatacccg ccatcaaaca
    17881 caagcgaata agccgtcgtc cacgtgcagg tgccaatatt tttaatacgc caggtctttt
    17941 cgaaggaagt attccgcacc acaggacttc catccggata agttatatct ttgacgaatt
    18001 ccgccgcatc acatctggac accggaactg ctgtggcata aatgggctga ttcgtggctg
    18061 tggcagcagg caacccagtc gggggcggag cagggctata tgtaggcaat ggcaatccag
    18121 gggttgcact cgctcctaca gcggcggtcg aaacctgttg tgtagtgcca gcttcaaggg
    18181 tctgagcaga agcggtgtaa agtccattta atgtggcaaa tgtatctggg gtcgctgtgc
    18241 tgtttccaag attacaggcc aactgggcca tcatcaaaaa cgtaacaaac agggaaatcg
    18301 gggctttttt catgaacatg actattctcc ggaagatgaa tttccaggga ttaagacgcc
    18361 tgtgaaagat aattggttcc gtccatttaa taaagaagcc agcttgcgct ggcttccaaa
    18421 tgtttatgag ggaaaaccgt aagatcagga acttccgcac aaataggtga attcagcatg
    18481 tggatactcc tgctccaccg ggtccacaat cacaatggcc atccattttg tgcccggcgt
    18541 gctgccaagg tgaagcttcc actcgcgcga aatggtcagc gtccctgcgg catccatctt
    18601 taatttcttg ggattggaat tgttattgtc actttgcatc cagtaatagg tgaattcgat
    18661 gggaccattg ctggtaaacg tggcatacac aatatgattc acattggcag ggcaccctgt
    18721 cgctgggtca cgtaccacat tgtattctac agaagtaatg gcaaaccccg ggttggctga
    18781 agaggatacg acgatctggg tatagaacgg ggcgctgtac tcacccactc caaactcaac
    18841 cccatccgga gtctgtaaca tccacttgga agtgtaggcc tcatcagctt gtggggctgt
    18901 gaacagaatt gaaatgggaa cgatctgtcc ggggtctact gcctgcggca ggttgtatac
    18961 ataggcacca ccaagcacat tgccatccca aaagatgatc ttataactgg tatcccatga
    19021 gcatggactg gtattcttga tctcccaagt cttggtaaaa ccctgccccg gtttgaagat
    19081 ggttccatct ggaatgttct cactaaccag gctggcctgc gcgcattctg aaacagacgg
    19141 gttacgcgtc cgtgtgggcg aggctactgg cgcaagtggg gtaaaagttg gggcaaacaa
    19201 caagggggtc ggtgtaaaag cgggggcttc aggagtgggg ttgcttacat tgccaagcgc
    19261 cgccacggtt tcagcgactg cggtttgtat gctgttggta tcctgctgtg tggtttcggg
    19321 ggtttctgca ctgccgctac aggctgtaat gagcagagac aagacaacga tcaaggtaaa
    19381 aagtaaatac ttgaattttt tgaacatttt tttcctttcg cacagataat tatactgtta
    19441 aacagaagcg cccggatttc ccgggcgctt tacgaaaatc ttttaaaagg ggttatggac
    19501 aggtaaaagt tgcgcgccca aactgtttct ggttcggtga gtcgatgtaa acatcaaccc
    19561 acgcgctgct gcccggtgta ttaaaggtca tgggaatgct cgggctggtt tgcgtacctg
    19621 cagaattgaa cgtcaggctg gcaggatagg ggtctgtgga gccatcactg aaaacgcggt
    19681 gcagggtgac ggtccccgga ccgttggtag tcacactaaa cgtatagtgg aaattgggac
    19741 atgaacctgt gacattgaac aagacactgg tcaccgtgaa cacaaagggg gttgaagtaa
    19801 cagtggctgt gggtgtaatg gtcgttgtaa cgcttggcga gccggtagtg gctgcctgca
    19861 ctttaacatc cacgtagaaa gacttccctt gatatccctt tacaacgggc acgaggacat
    19921 tgccattggt aacgatacgc caatatccct tgtattgccc caaagtggca ggagcagtga
    19981 gattcacttc aaggtcaatt tcctggcctg gcgccacagt gctgatgggt tttgaggcag
    20041 ggccacccat tgagtcgccg ctatcaaaga ccattgagaa accagtccag gtacaagagc
    20101 cgatgttctt gatccgccat tttttgacaa aagcctgatt gggggtcacc acagttccat
    20161 cagggatggt gacgtcagca acgaactcag ccacattgca gttggaagtt gccgtagcga
    20221 tcggtgtatt cgtcaaaacg ggaagaggaa tgggggtaag ggttgcagtt gtaaccgggg
    20281 gagccaccgt aaaggtggga gtattgttta tgataattgc aggggtcgcg ctcaataagg
    20341 cttctacagt ttgggcagcc gaggtacttg ctacatcttc cgcattgttt gagggcaaat
    20401 tacatgcgct caaaaacaag gtgaagatca tcagcagatt aaataagcgg atttgtttct
    20461 tcatgatttt ctcctttgta gtcgtgatat tgtaccgcag gtcaaatttc gagaattttg
    20521 cataaattta gacatcctgc tgccaccatc aagtttttag ccgtgaatta agcgaatgtt
    20581 ctaaactcca ttcatcatac gcggatcaag tgacagctcc gattgtctta taaaaatgta
    20641 tgcagacctg gcgggccact tgcattaacg cctataagct ttaatttgtt cccaagacaa
    20701 attttgaatt cagacaaaga atgcgtagtt tgcaaggaaa caaaaaaata gtgcaaaatc
    20761 ttgtgatttt gcactatttt gcttatgcgc aggtttttat tggccttgaa cctgaaggat
    20821 gtttaccacc ccattcaatg ggtagtacgc aatcaagatc ttgtttttcc catgtgtgaa
    20881 caaagccaca gtacacaaat agcctttctg tgtcttcact tcctccgttt tttcaaggac
    20941 ccagccattg tttttggcct gctgatcata ataggcaaaa acggtatccc aggtagttgt
    21001 agctggcaca gccgacatca attgcaagtc accgctgatg cctgattgtt cgcgcaggat
    21061 ggcaacaaaa tagttcacat catcttgagg ttcggcttca cccggcattg gagggaacat
    21121 gaaaccatcc attggatcgg gcgcgccacc atcagtagag tcggcagggg catccactac
    21181 cgcaccggga tcagcagggg cgccgctatc agcaggagca ccagagtcaa cgggggcgcc
    21241 agaatcggcg gaggcttctg taggagcggg gttcccgcca ccacaggctg atatgctgac
    21301 aaccagcaca accaaaatca gcatatatat gtatttcttc attttttatc tccttgatat
    21361 ttggaacatt acaaaaccac ttccctgctc atcttcaaca ggataccggc aagccaaaag
    21421 acggccagtc ctgttttcgt ttgaaacgca gggaagtgat attttacaac aatctgtttt
    21481 ttacgctggc gaagattcca cgtgagcgcc agccatccat agaccaagct gttcacgagt
    21541 ggttttcttt gcttccacta ctgtcacaat ttgtccacga tacatgacag cgatgcggtc
    21601 tgaaagcgcc atgatctcgt ccaactcagc ggaaataaga aggacagcca ccccgcggtc
    21661 acgcatcttg atgatctcgc tgtgaatata ttcaatggaa ccgacgtcca gaccacgggt
    21721 gggttgattc gcaataacga gcttcacatt gctgcgactt aattcacgcg ccacaatcac
    21781 cttttgctgg ttgccaccag aaagtttgcc agcgctgaca aagggagatg gcgtgcgcac
    21841 atcaaatgca ccaataagtt tgcgggcatt ctcatccagc gccttttgct ggagtacacc
    21901 acgattgctg aagggtggtc gataataatc gcacaaggcc atattatcgg caatggaata
    21961 ggagagaaca agcccgtggc gctgacgatc ttcggggata tgagccagcc cggcttcggt
    22021 catgatccgt ggcggtttgc cggtcaggtc aatgccatcc atggtcactt ttccgcctgt
    22081 cggagaacgc aacccggtca atgcttctga aagttcagtt tgaccattgc cttgaacccc
    22141 ggcgatgccg agcacttcac caccgcgtac cgtgaatgaa acgttgtgga ctgtttccag
    22201 gttacgctgg tcgcggaccg agaggccttc cactttgagg atatcgccgc tggcggcgtg
    22261 gtcatctttt tcaacggtga gaatcacctg gcgaccaacc atcatattgg caagctgtgc
    22321 ttcgttcgtt ccctgaggct gaaccgtgtt gatgacccgt cccgcacgca tgacggtgat
    22381 acgatctgca acagcaagga cttccttgag tttgtgtgtg atgaagatga tggaaacacc
    22441 gcgtgccgtc aggtctcgca tgattctgaa gaggtcttca gcttcctgtg gagtgagcac
    22501 ggctgtaggc tcatcgagga tgacgatctc tgcattgcga tacagggttt tgacgatttc
    22561 aacacgttgc tgcaaaccaa ccggcaggtc acccaccaat gcatttgggt ccacatcaag
    22621 gccatattgt ttggatattt cattgatacg ggtggcaaca gctgcacgat ccaacgaacc
    22681 gcgtttgacg gtctcatcac ccagcatgac attttcagcc acggtaaata ctggaatgag
    22741 cataaaatgc tggtgaacca tgccaatgcc tgctgcaatt gaatcgttcg gggaatcgat
    22801 ttcgatcgtt tttccattga cagcgatctc accagagtcg gggcgatgca ggccatagat
    22861 gagattcatc aacgtgcttt ttcccgcacc attctcaccc aacaaggcat ggatctcgcc
    22921 cttacgcagg tcaaaattga cattatcgct ggcaagcact ccggggaact gtttggtgat
    22981 gttcctggct tcgagaacga ttttggcttc tgtcatttcc gcctcctatt tctcgtatgg
    23041 aacgccatct gcagcgggcg gcgtggtctt gccgatgata cccgtcagaa tgaacatggt
    23101 gaggatgtat ggagtgaggc ccacaatata ggattcctga aggaaagacc attggggtgg
    23161 gatcacatca cggaagatct gcatattaat gagcagcgcc tgcgatgcac cgaaaaccag
    23221 cgaagcagcc catgcgccga tgggcgtcca gtttccaaag atcatcgcgg ctagtgaaat
    23281 aaatccgcgc ccattggtga gcaaaggctc aaaggaggga accgactcga tggtaaagta
    23341 cgcgcccgcc agaccagcaa acatgccgcc gatcagaaca tttgcataac gcatcttaat
    23401 cacattgata ccgacggtat cagccgcttt gggatgttca cccacggcac gagtgcgcaa
    23461 accccagcgg gtattgaaca gcacgtaatg tgaaaaaaac acaagcaaaa tggcgctaat
    23521 ggcaatcggc ttttgatcaa aaacgcggtt gatcgctgcg agattttcaa ggcaggaagc
    23581 agaagaaccg caaacagatg taaatatctc agtaagcgga acgtggatgg tgggaaggac
    23641 gcccggggaa ttcggcacag aaccgccaaa aacactgcct tttccaaaga acaattgacg
    23701 attcaaaaag ccagtcaacc caacagccag gatattgata acagtgccac cgataatctg
    23761 atccactttg aaggtgatgg aaaggaccgc gtgcagcaga gacatcaaca ccccggtcaa
    23821 aatcgcaaaa aacacaccca tgccaatgct aaaaccaact ccaaaaccaa agacgtggta
    23881 catgtaaatg gcacccagcc atccaaaaaa cgcagaggtc aacatcatac cttcaatgcc
    23941 gatattgacc accccggccc gttcacaata gaccccggaa agagcgccca gtgccagtgg
    24001 ggtggcaact gcaatcgtgg tagccaacat actggtaatg atcaatacag gcggttgttt
    24061 tacctgtccg atcatgacga ctgtgccgaa aagaacaaca atgatcaggg caataaagcc
    24121 aaaacgtttc cagtccattt tttctcctag cctccccaac cgcgggtgag tacgacacgt
    24181 tcacctttgg atttgatgcg atagatatat cgcacgatcg catctgctgc aacaaagagc
    24241 aggatgaaag cctggatcat ggagatcaga tccacaggca tctgggtgag gaactgcatt
    24301 cgtgtcgctc cattacgcat cgcagcaaac aggaaagctg ccagcaccac ccccaaagga
    24361 tgtgattttc caagcagagc gatcgcaatg gcgtcataac catagccttg tgaaaagcca
    24421 agttcatgac ggtaattcaa accagtcacc tcgatcgtgc ctgccagacc agccaacata
    24481 ccagaaagcg ccatcgtaag gatgacgatc cgcttcacat tgatgcccgc atacttcgcc
    24541 gcatccggat tcaaccccac agtgcggatc tgaaaaccga gggtcgtttt gtttaacagc
    24601 caccaaatcc caaatgcaac cagcaaggcc agaatgaacc cccaatgcac acgcaagcca
    24661 tcaaaaatgg gagggatgcg cgcactatcc gcgataagcg gagtgcgtgc aatcacattc
    24721 gttgggctgc gatctttcat cacaccgttc aacaaatagg aggttgtatt caacgcgata
    24781 tagttcatca tgatggtgtt gatgacttca tgtccgcctg tataggcttt caaccagccg
    24841 acaatagccg cccacaggcc gcccatcaac acaccgaaaa caatggccaa cggcagatga
    24901 ataatggcag gcaggtcata cccaagccac tcgggcaggg catagccaac caatgacgag
    24961 gcaaccgctc caacagccag ttgaccttct gcaccgatat tgaacaaccc acccttgaag
    25021 gccagcgcaa cagccagccc cgcaaagatg tatggtgttg cccaaacagc ggtttcactt
    25081 aatgccttcg ttgaaccaaa tgagccttca agcaaacccc gataggccag aattgggttt
    25141 ccgcctacaa acgcgataat cacgccgcca acaactaccg ccgtcagaat cgccaaaaat
    25201 gggaccaggc tgttatctga aatcgtctgc aatatttgtt ttatcagcga atttgattgc
    25261 ttttctgatt gcatccttcg ctccagttga actgtgaatt gtaggacaat tatatcaaac
    25321 aagccgtctg gaaccccatt atcggtatta caaaatcaaa taaaaaaggg aaagagactc
    25381 gcgcctcttt cccttttctt acttcgtcaa aaaattacgg cttcacagga gcaacattgg
    25441 tcttgatgga accatcgagc agacccttgt tgatctcttc catcatggtc ttgacttcag
    25501 cggggacgct gctgtcgaga tcgtggaacg gagcgtaacc agcggcaccg aagacgttac
    25561 catcagcaat ggagccttct ttggtggcct tgatcaggtc ggcaactccg ggggtgatga
    25621 gcttcatcgc gctggagagc atgcgcggag cagcttcggg cagggtcagg tactggtcgg
    25681 tgtccacgcc gatggcgtag gcgccagcct gagcggcggc ggtgatggcg ccgttaccgg
    25741 tcaaaccacc gcaaccaaac acggcatcag caccctgatc catcatggat ttggcggtct
    25801 gagcgcccca ctcgggatcg gtaaaggtct tgtcgaagcc cacatcgctg tggtacacaa
    25861 cgaacacctc agttgtggtg ccgttcttct catcggcata cgcagcacca gccttgtagc
    25921 cttcaccaaa gcgccaaacg gggggaacca catcggtacc gcaaacggcg ccgatcttgt
    25981 ggctctcaga catcatcgcg gcaagggcgc caacgaggaa gccagcgtta tcttcaggga
    26041 agttcaaacc agaaacgccc gcaacggtct cagcctggaa ctgatccaca ccgatgaagc
    26101 gaacattcgg gtaggtggca gcggcagcag cggtggcttc accgagaccg aaaccgacgg
    26161 tcacgatgac atcgtagccg gcatcaccga actgagcgat gttcttggcg taatccttgg
    26221 catcagacgt ttcgatgaat tgaacaacat cggctacgcc atcagcctga gacttctgaa
    26281 cgccttccca ggcagactga ttgaaggact tgtcattgat cttgcccaca tcggtcacga
    26341 gacccacgca gaacacttct tccttcgagc agtcttcagc ggtcggagca cttgcaccgc
    26401 aggcagtgag gaccatcgaa gcgacgatca acaaagccat tacaaagtaa agctttttca
    26461 ttttttcttc tcctaaaaga atagggtttt aaaggtctgc catttctggc aaacctttac
    26521 gacagaacaa gtataaatcg ttatatttca ttgtgcaagc ctgaactatt acaagcatat
    26581 ttcaggcaaa atcacggttt ttcgcgggat acccctgtct gaatttcacc atttaaaagg
    26641 gcagaaagca gggaatccaa ctcacgcgtc aaaccttcgg aaaatttttc attaagcggc
    26701 acatacccaa acgaaccgat tcccgccttg gaaagatttt ctccctgaat catgcgcatc
    26761 acctcctgga cctcgaaact ggcactgcca taaacagaag tgaccacccc gtaaccttct
    26821 gcactctggt cgcgttccgc gccgatcgcc agcacctgcc catccatcgc tgcacgaagc
    26881 gccccctccc ctgtcgcccc acccgcagcg aagatcacat ccgccccgcg ttgaattaaa
    26941 gactggcctg tttcgtaccc ccaaacctca tccagaaaaa ggtcatcacg gctggcgtca
    27001 tcgcggtaaa acacaagcac cttgatgttt tcatccaccg actttacccc cgcgttgaat
    27061 ccctcacagt agcgccacat cgcgtcaatg tcgctggttt cgcaaactgc ccccaccacc
    27121 cccgcaccag acagccgggc cgccaacgcc ccggcgagaa aacccatctg atcttcggca
    27181 aaattgacag ggatgaggtt cttgcgtgcc tctttttggg gttgggaaat gcccacgaaa
    27241 acagaatcag gatacaggtc cgcactacga agcgtttcat catccatacc ggggccgatg
    27301 gtgaaaatca catcgacccc ctgttcagca aaaaacacga tattcttctc ataatcgcgg
    27361 gtatcaactg attcgatata ttccaccctg tcactcaacc cgccagattg agcctcctgc
    27421 agccccgccc aggtttcctg attcataccg tgatcttcga ggccgcgtgt atcagtgacc
    27481 agagccgcgc aaaagacctc ttcactaaaa caatcttgcg attgcatgca agccgtgaga
    27541 gctgacatgg caatcgcaag aagaaatatt tttgatctca attccaaatt cctgatctct
    27601 aaactgcctg cgccgcttct ccgcgtttga cgccaagcat caaccacaag gcgatcatca
    27661 tgaaaaacgg tgcgatcagc ataatgatgt tgtagtttcc attggtcaat tgaatggccc
    27721 agccattcac attcgggcca acgatggcag agaaagtgga aaacagataa tacaaccctg
    27781 taaatgtgcc gatgcgcgcc gcgtcagtca ggtctacgat catgggcagg gaattgatgt
    27841 tgatcaaagc ccaagccgca ccgccaaaaa tgagcagaac gcccgccagg gtcaacatgc
    27901 gtggaccgcc ttcttcaagc ggaatgccaa ccaaaggcag cggagagatg cccgtcagca
    27961 gggctttggc gggggtgata tagagcaata ccagcatgat agtgagagtg atcaaaccga
    28021 tcgagatggt ggtacggcga cccagcctac ccgcgataaa accagatggg atcgcaaaca
    28081 ggacaaagaa caaaggcagc accgaaagca gggttgcccc gtcaccttca ttcaacccga
    28141 ggtgattctt ggcataaagg gtgaaaaagg tttccactgc tgaaaaacca atgaaccaga
    28201 aaaatatggc aaaaagaatg cggaggccgc ttttatcttc actgctgaca acttcgcgca
    28261 gggaggcaag catgcccggc tgttcttcac ttgccgcata ctcttttggc tcttcgataa
    28321 aaagataaac gatcaacgcc gagagcaaaa ccaacccgga gccaagccag aacggaaaat
    28381 tcgggcttaa cttgtacagc aggccgcccg tctgcaaagc gacaatggta ccgatgccgc
    28441 ccatgaaatt gatgatgcca ttggcctgag agcggaattc agaaggggtg atatcgggca
    28501 tcaaggcgat gacaggtgtt cgccagaatg ccgcgctcaa cagcagggta cttgtgcaag
    28561 ccacaaacaa cggcaatatg gcaggcaatg ggatcaggcc aaaggctaca gccgtgatcg
    28621 gtgcgccgat cagcaaaaat gggatgcggc ggcccagggg tgtccgcaat cggtcagacc
    28681 atgcgccgac aggaggctga ataaaaagcg cggcaatatt atccagcgtc atgaaaaagc
    28741 caatgaccac cggagacagg ccaaattttt ctgaaagaaa gatcggaaca aaggcgttgt
    28801 acacacccca aactacgctt aaaccaaaaa agccaaagcc gagcaaaaag gtcttaccgt
    28861 aattcaatct tgatgacatc gtttcctcca tgcagtggat atgggatggg gttcagattg
    28921 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naggttgaag gtttcaggtt
    29041 tttgagcttt tcgatgaatt tcaggtcttc ttttgtcaat tccgcttttt caagcatgtc
    29101 ggggcgtttt ttgaaggttc tttccagagc ctgttcgcgc cgccatttgt caattttggc
    29161 atggtcaccg gaaagcaaga tatcgggtgt acgccagcct cgaaattcag gcggacgggt
    29221 gtaatgtgga tactcaagca ggcccatggc atgtgagtca tcctgtgcgc cggtagggtc
    29281 gccaagcaca tcaggcaaca gacgggaaac cgcatctatc aacatgagtg cgggcaattc
    29341 gccgcctgtc aacacaaaat cgccgatgga gatttcgtca gtaaccaaat gctcgcggat
    29401 gcgttcatca aaaccttcgt aacggccaca gatcagggcg atgtgcgggt gtgcggaaag
    29461 ctcctgggca accgcctgat tgaacaaacg tccctgcggg gtgagcaaaa tgaccgggat
    29521 gggggaagaa tcattctcat tggatattcc cagcacagat tccaccgctt cgaaaacagg
    29581 ttcgggtttc attaccatcc caccgccgcc gccgtagggg gtatcgtcgg tcacatggtg
    29641 tttgtcgtgg gtataatcgc ggatgttgtg tacccgcaca tcgatcaatc cccgttcacg
    29701 ggcgcgtttg agaatactgg tatcaagata ggaagaaaaa acttcgggca aaagggtgaa
    29761 cacctcaaat tgcatgcggc gattttagca tagaaacctt gcaggacatc cttttagcca
    29821 tccacgggtt ttagagagtg tcgcactacc aatccaaacc attcggagtt tcaagtgaat
    29881 aattatattg ccctcgtcat cacctttgcc gcctctctat tgtttctacg atccatggat
    29941 ttcattgccc atcgcggctg gatggacgga aaactcagtc gtaagatcat ccacattggc
    30001 acaggtccgc tttttgtgtt gtgctggttc tttttcaacg atgcacccga agcacgctgg
    30061 cttgcggcgc tggtgccttt tgctgtgacg gcacaattcg ccctgatcgg gctgggagtc
    30121 attaaagacg aggcctctgt gcaggccatg tcgcgcacag gtgacccaaa ggaaattctg
    30181 cgcggaccgt tgtattacgg catcatgttc gttgtattga cgctggtgta ttggcgagag
    30241 aaccccaccg gcatcatcgc gttgatgatg atgtgcggcg gtgatggcat tgccgatatc
    30301 gtcgggcggc gcatcgcctc acccaaactg ccgtggagca gggaaaaatc cattgctggc
    30361 atggtcagcg tttttgcggg cggctggttg atggcaggca ttatgatcta catttatgta
    30421 gcggctggtg ttttttccgc tccatttgcc caatacactt tgccgatcac gctcattgcg
    30481 ctggcgggtg cagtggtcga gtcactgcat tacaaggaca ttgacaatgt gaccatgaca
    30541 ctggcctcgg ctttgatcgg cggtttgttt ttctaagaaa gcgcctgaaa gtgtttttgt
    30601 gtctacgtga ttgaattttc aagcttaagg aatcagccgc gaattatttt aaaagtctga
    30661 aagattaacc acggagccac agagaaaaaa taaaaactcc gtgaccccgt ggttcaaaat
    30721 ggttgttcaa agaaattccc tgcaacgtat gtgaattgca agatacggac aagatataaa
    30781 aaagccgacc ctgtaaaatg gatcggcttt ttcgtgtaaa tccgcggcta aagaattgaa
    30841 tgattagatt cgggatgttt catattttgc gtaagaactt aagcagagaa tcggctactc
    30901 gcccggtata tcatcctctt ttttcaggat catcacttta taagattcgg caaactcgag
    30961 gcccttttcc tttccttcct ctttggccca ctcgtgcatc caatcatcca catcatgggt
    31021 gtcagactgg ctgacatgtt tcttcagtgc ttcgatcttg atatcgatgg tttcgctgat
    31081 atcaacccag gtatcgggct tttccgaccc gtggatatac aaccgtttga cattgtgcgg
    31141 ttcatacccc gcctcaagca ggtcggtgaa aataagcctt gtcccagcgg aagggaagac
    31201 cgcgtacgcg gctgcttctg ccgcagcacg atgatccggg tggttgatgt atccgctgcc
    31261 gtagaaccag gccgaagggt cgcctgaaac gactacgtca ggtttgtata tgcggatgag
    31321 gcgagtcagg tctttgcgca gggcaatgct ggcggtcaat tctccatctg gataatgaag
    31381 aaaaaccgtt tcagaaattc ccagcaccgc attggctgcg agttgttcgc cttcgcgcag
    31441 ttctgcaagc acggatttat agttggcacc tttggtcaca tcgtttgaac cagagtcgcc
    31501 gctggtgata agcacggaga tgatcttgca tcccgctttg gcccatttgg ccagcgtacc
    31561 cgcaacagta aagtcttgat catcgggatg cgcctgaatg gacattgcaa tctttggaag
    31621 atattcctgt tcgtttgaca tggctgtgat tttaccagag gcttaaagtc agagcgccag
    31681 gggctgccga ccctggcgct ctttctacaa ggaggagaag agaaatgagc ttgtgcctcc
    31741 tactttatca caggcattca agcactactt gacgccaacc ctacgcctct cccatgatta
    31801 tgcaacgtct tccctgcaat gaaccgctga ccagcaaaac ctttgtgact gcaggcaggc
    31861 tggtgatttt gaaacgcaaa taatgcgaac agcgagtttt cataatttat ccaagttgcc
    31921 atttgcgcaa aagcagggaa aagaaaaacg tcagccgcga atggcattaa aaaattcacg
    31981 gctgaaaact tgattttgat cacagagaat caacttgcta attgctttac tcgcagcatt
    32041 cacattaaag ctttttgact ttgcaacggt cacagcagac tctatatggc tttctcaaat
    32101 taatatctga caagcctttc gaaccgccaa gcccgtgaag aacgccaaga aaatccatta
    32161 aacttcgcgc tctttgcggt tagttttgtc tatgttgggc cagtactccc gactttgaga
    32221 aagccatagc agactctaca tccatgcttg acacatccac aaattgctcg tagactctca
    32281 attaagttct gccttaatcg aaagataccc atgaaaactg tccaacccat tcaatccttt
    32341 gaccagatca aattgctggc agatgcacgc cgaatggaaa ttctgcgcct gctgatggcg
    32401 gcccccgcca ccctcacaat tcttgcgcaa accatgaagc agtcgcctgc atgggtgcgg
    32461 caccacatcc tcgcgcttga gtctgcggaa ctgatcgaag tttctgaatt gcgcaaaaca
    32521 ggaaaagtga ccgaaaaatt ctaccgcgcc aaagccgcgg cttttctttt acaggaagtg
    32581 gtcctgccga aaagcaaaaa gcaatccatt atcttctctg gcagccacga tctggcgctg
    32641 gaagagattg ccgaacacct caataaacat gtcaccttgt tgagcatgcc cgtcggctcg
    32701 ctggatgggc tggtcaattt acgtcagggg ctatgtcaga tctccggggc gcatattttg
    32761 gatgcaacag gcgaatacaa cacccccacc gtccgtcact tgttcccaga ccgcaacgtg
    32821 gaattggtta cgctggcata ccgcacgcag ggcttgatcg ttgccccagg caaccccagg
    32881 aacatcaaaa aaatatccga tctggctcgt gagaatgtta aattcatcaa tcgaaattcg
    32941 ggctcgggca cacgcatctg gttcgacaac gaactgaaga aactaaaact cgacccgaca
    33001 caaatccccg gttacgacca atcggtcaaa acccatagtg aagccgcaca ggcagtcttc
    33061 accaaaactg ccgatgtggc ccttggcctg caagccgccg ccaaacaaca cgacttggat
    33121 ttcatccccc tctttgaaga acgctacgac ctgattctgc cgcgtgaaaa tgaaagcctg
    33181 ctttcaccgc ttttggacta cctgcaaact tcaaccttcc gctcatcgct gggcaggctg
    33241 gtcggttaca acccaactca cagcggcgaa cagatcgccg tgtaaatcaa
//
LOCUS       DAOQJC010000037        33223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_584_length_33223_cov_11.244875, whole genome shotgun sequence.
ACCESSION   DAOQJC010000037 DAOQJC010000000
VERSION     DAOQJC010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 33223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33223
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_584_length_33223_cov_11.244875"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="PLE39_07575"
     CDS             <1..1314
                     /locus_tag="PLE39_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNE68223.1"
                     /translation="ELRIRAVLEVPMLYGGELIGVLTADEINDSTRVFTESDERLLTL
                     FASQAAGAIHSARLREQTAQRVNQLQALHTIDRAISSSFDLRLILNTVISQSISQLGV
                     DAADILLFNPYLQTLEYVAGQGFRTRNIEQSRLRLGEGQGGQVALNRTVLHVSNLVKA
                     GTEFTRASLLVGEGFLEYYGVPLIAKGEIKGVLEVFHRTPLHASSDWLGFLETLAGQA
                     AITIDQTQLFDDLQRANLELIIAYDTTIEGWSHAMDLRDEETEGHTRRVTEMTLALAN
                     AMGISDDQMLHIRRGALLHDIGKMGIPDKILLKEEKLTEAEWKVMRQHPNYARDMLQP
                     IKYLQKSLDIPYCHHEKWDGSGYPRGLKGEQIPLVARIFAIIDVWDAVTIDRPYRKAW
                     TKQKALKYIREQSGKHFDPSVVAAFLKMFDKKDPPAGKPAKRKNK"
     gene            1393..3078
                     /locus_tag="PLE39_07580"
     CDS             1393..3078
                     /locus_tag="PLE39_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68224.1"
                     /translation="MIYRHFKHETWLYVLVLLIAVSVRTIQLGAMPLSDSEAAPALQA
                     LQITQGQRPLLSPHPFYILSTAVLFFLYGEGTNFLARFSPALIGSLLVLIPLLFTERI
                     KPRPGLILALFIALDPGMVALSRQAASPIFAITFLLFAIGFYENNNLKLAGSFAALAL
                     LGGPSIWLGLLSIGIAWAIYRGVQLRGSEEQASVNVKEALWPFVVTLVVAGTFFFLIP
                     NGLSAAFSSIPEFFKRWTIASDFPASRFLIALAVYQPLGILLAVIALIRGWQHGSRRI
                     ISLSLWFIVALLLAIFIPSREIADISWALIPLWGLAALELIRNLHIFPEERNEIFGVV
                     FLTAFIWVFAWLDFSGMVWLPNTTREYMMRFWLLVGALLLIAISLLLVAAGWSIRTAR
                     IGGVLGMALVLGALGLGGTFGAAGLRGLDNPEIWWQAPLPVQAKLIQASVNDLSEWGT
                     GNDNMAPVIIAGLDSPALEWALREHQVEVVETLDISSAPYFVITPLQNDPAFASTYRG
                     QDFTWRKTFLWNETLPQDWIRWVTLREVPQTGESIILWARDDLFLDKANQSIP"
     gene            3093..3776
                     /gene="cmk"
                     /locus_tag="PLE39_07585"
     CDS             3093..3776
                     /gene="cmk"
                     /locus_tag="PLE39_07585"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNE68225.1"
                     /translation="MTNKPTPSIIALDGPAASGKSTLGKRLADALGFLFFDTGIMYRA
                     ITWIALQHDMDLRNEAAVSQLAQNAQIDIRPPSKNDGRACDVIIGEKDVTWDMRGGDV
                     DANVSVVSAYAKVRQALSEQQRRIGMRGNVVMVGRDIGTVVLPNADLKIYLDASAEER
                     ARRRYNEIIARGEPANLDEILKKVIERDRIDSTRAVAPLRPADDAILIDSDAMDAEQV
                     FAYVLNLCK"
     gene            3767..4471
                     /locus_tag="PLE39_07590"
     CDS             3767..4471
                     /locus_tag="PLE39_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE68226.1"
                     /translation="MQVKDYRIPFHIQANRLWLRFGIRTLFNILGSVKITGAENIPLG
                     SPYVIAMNHISIFDPPLVVSFWPEEPEVIGASDVFAKKGQGTILSLYGVIPVHRGTYD
                     RTLLEKIILILKQGRPLVIAPEGGRSHEPAMRRAMPGVGYIIEHAGVPVVPVGLVGTT
                     DDFWQRSKQYWRGQGEKPRLEMRIGKPIHFQPILEKGAERREARQKNADLVMQHVAGL
                     LPAEYHGVYTGQAIST"
     gene            4474..5220
                     /locus_tag="PLE39_07595"
     CDS             4474..5220
                     /locus_tag="PLE39_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE68227.1"
                     /translation="MDTPTPKPVSEVWRPELVRLPKMTHARRAFRVFGHGLTKLVAKL
                     CLHVTVEGLENFPRKAGAEPVLVVINHLGDSDVPALISSLPFPPDALAKVELYDLPIL
                     GKLMDWYGVIWLHRGRPDKRALRAALDGLAEGRVIVIAPEGRYSLTGALEEGNNGAAF
                     LAYKSGAAVLPIAVTGTENETVYGNLKKFRRARVNLRVGKMFKLAGQAQGGERGGAGE
                     RQEVIAQGTRQIMEALAGLLPEKYRGAYSG"
     gene            5296..8610
                     /locus_tag="PLE39_07600"
     CDS             5296..8610
                     /locus_tag="PLE39_07600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE68228.1"
                     /translation="MGSLSTLPASIAFPAPPTLAGWAVWLSLLGMLAYALYHWRAYQP
                     AMKRIEWGFLVGLLLLTLVANVFILRPFASSARPLPNLPVQPPGSALIVFSAIPWLLS
                     GGLIGPLGAAAVGALAGLLRGTLDTYSLFSIIEFALMGAWFSTNTRQRFRTPVYSLLR
                     QPIVGALLLIPLHALFYIVVALFTQWGVDIPTTARLDFAISNTGIVTLAFGIEMLVGG
                     IVAQIISMSFPAVWGGKQQALQPSPGEKSLESRFQFAVGTFISLLLLTLLIGDWVVAG
                     QAARDMLQDRLTSAGEAAAQSIPFFLETGQNLAVQLAADPRLLEATGDELHSIIGSRI
                     QAVPYFDQFFVLEANSETLLAGYPDDSVTTFKLYPDESMGLILAANGVLTQIYSIPPI
                     SADESARVSFLVGIVDKFGSVQRVLIGRTTLKTNPLTLPLIEGIDNMKNLNGAGFLLD
                     ESNRIIYHSETIEMAATYNGQRSTEAVFYDDTAADGTRQLVYYQPVTGRPWAVVLTVP
                     AQQAQQLALNIAMPLSLMILVLAVVAMISLRVGLRVVTGSLQSLATEANRIAQGNFDH
                     PLQTEGVDEVGQLRRAFEKMRSSLQARLNEINSLLRVSQGVASSLEMQDAVKPVLDAI
                     LATGANSVSVVLSPSILPDTFVELPSRFAVGTVQDIYAHLDDQILALAQSQEKVVLPN
                     LSRSRELELDPSLPQPLSLLAVALRHENRYYGVVWAGFEQPRMFSDSDIRFMTTLAGQ
                     AALAVANAHLFLNVEASRRQLKAILNSTPDPVLVTDHRNRLLLTNRAAAIALGQSEDT
                     TSDIGRKTEQVVKIRPLLALLESATTENQSTEITLADKRTYLATASAVMVEGKQIGRV
                     CIMRDVTHFKELDMMKSEFVATVSHDLRSPLTLMRGYATMLEMVGELNEQQQGYVKKI
                     ISGVENMSRLVNNLLDLGRIDLGVGLQVENVSVLDIIERVTGALQLQASQKNIQLTVE
                     LSKDMPHAIEADQALLHQAVYNLVENAIKYTPNDQRVTIRTETQPGSLTFIVEDSGIG
                     IAADDLPRLFEKFYRGKQREARAQHGSGLGLAIVHSIATNHGGKVWVDSVVGKGSTFY
                     LQIPLTQPKENKRIGP"
     gene            8722..10032
                     /locus_tag="PLE39_07605"
     CDS             8722..10032
                     /locus_tag="PLE39_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HNE68229.1"
                     /translation="MKLIRISLFTLMIALSAFQLAAAQGDDPLAVVMTAEGTIMPPML
                     EYFKRGIETAENSNADVLVIQLNTPGGSIETMLDIIQTMRASKVPIVVYVSPNDAMAG
                     SAGALITMAGHASAMAPKTAIGAASPIDSSGADIESTLETKIKEILKAKARVLVEARG
                     EEATRLAEAMIDDAKAVTASEALEANLIDFVSDDTEDLLQALNGFTVKMDDGSEVTLN
                     TENIRTEELGISFIEQLLMMLTDPNIAFILLAIGVQGILIEISSPGGWFAGFLGAVCL
                     TLAVYGMGVLTVNWFGFIFLVIAFVLFILDIKAAAHGALTTAGVASFIVGALVLFNSP
                     GTPQFQRVSVPLVIGVGVFIGALFFAIVLVALRSRHNPIQTGVESLLGKTGTAKTFEE
                     EAGQVQVGSELWSAEKAPGSESIRKGDPVEVVEVRGLRLIVKKK"
     gene            10066..11004
                     /gene="lipA"
                     /locus_tag="PLE39_07610"
     CDS             10066..11004
                     /gene="lipA"
                     /locus_tag="PLE39_07610"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNE68230.1"
                     /translation="MPATPTRDRVKPETEARPLRRPEWIKVRAPSGETFERLQVLMRS
                     KELHTVCEEAMCPNLGECWGSGTATFLMLGDVCTRTCAFCDVKHGKPLLMDWGEAERV
                     AQAVKSMNLKHAVITSVNRDERRDGGAPIFAMVIRRIREVLPGCSIEVLIPDFKGSIE
                     ALKIVMDARPEILNHNVETVPRLFKTVQPQDNYEWAAATLSNAKKLDPDVLTKSGIML
                     GLGETMDEVKQVMRDQRSWGVDILTIGQYLQPSKKHMPMDRYYTMEEFAELKAFGMEI
                     GFKWVESNPLVRSSYHAAEQVRALSIVHRKLYGEKA"
     gene            11226..12266
                     /locus_tag="PLE39_07615"
     CDS             11226..12266
                     /locus_tag="PLE39_07615"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNE68231.1"
                     /translation="MYTTPKHIFLRSLLLAAYILALVIQPFSARPAFAAPSLQTINYQ
                     PTLTATPASGPAPLTVNFSRYVATDPNGPLVSYVMLDFGAGPGLGAEIDFGDGTSTFI
                     LDKTSSMVYNLPGTYTAVMTVFYNNSTSITTTLIITVDPSVTPEQLPLIVASPASGTA
                     PLVVNLTSSATMNPNGPLINNYKVDYGDGTSDLYFFLDGAPSFINNGSHTYNTPGTYT
                     ATLTVTYNNATSASATTTIVVNPVLSNVVRSTAVNLSATLKKTTVNVSGTVIVQNENG
                     TVIPGAIVSITWVKPNGRVVTQNAKTNANGVAKFSTSGNRGTYTLTVTNIRKTGYSFD
                     SINSVVSGSITK"
     gene            12379..12909
                     /locus_tag="PLE39_07620"
     CDS             12379..12909
                     /locus_tag="PLE39_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE68232.1"
                     /translation="MKKGFIFMNILETPRLILRNANADDAPFMLDLLNQPSFIRFIGD
                     RGIRSLDGAREYIETRLVKSYKDNGFGLYITLLKEENLPIGLCGLVKRDGLDDIDIGF
                     AFLAQHESKGYGFESASAVMEYSKNTLGLKRIAGITTPDNEKSIGLLEKLGLKFEKMI
                     KLPNDTEELKLFIRTL"
     gene            13056..14198
                     /gene="phnD"
                     /locus_tag="PLE39_07625"
     CDS             13056..14198
                     /gene="phnD"
                     /locus_tag="PLE39_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNE68233.1"
                     /translation="MNKRIAFVLLTLVVAISMMLSACGPAATEAPVATEAPTEAPTEA
                     PTEAPVATEPPMPAIGSPEHPIKVLFVPSVDANIIVTGGEVMAAALNEATGLTFEVVV
                     PTSYAATIEEMCASPSDTMAFIPGLGYAIASQLCGVDVSFKAIRFGFPVYWAEYIVAR
                     DSKYQTLADLEGAKWGYGDQGSTSGYMVPTVELAAAGITPGEQVQTGGHNQTVTAVYN
                     GEVDFGTVFYSVPLTPEGAPLFSYDDFVAGKITSLEQYEIPTESVPNCAPDTEGKKLL
                     CDGWRILDARANIRTEAPDVIQKVRILGVSAAIPNDTLSFGPEFPADLRTQIEEALLA
                     FAATEAWGTSIGSADFYGWSGIAPATDAEYDMVRAMVAATGYEPPK"
     gene            14378..15130
                     /gene="phnC"
                     /locus_tag="PLE39_07630"
     CDS             14378..15130
                     /gene="phnC"
                     /locus_tag="PLE39_07630"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HNE68234.1"
                     /translation="MLQVKNLTKIYDGGVKALDNVSFEVPKGQFLAVIGLSGSGKSTL
                     LRCINRLIDPTEGQILWDGEDVTAATPEEMRLIRRKIGMVFQHFNLVHRSKVITNVLA
                     GRLGYVNPAWSFLNRFPKEDIEKAIKQLERVGIADKADQRADELSGGQQQRVGIARAL
                     MQDPNMILADEPVASLDPVLAHSIMKHLEEINKKDGVTVLCSLHFLDLVHRYADRVIA
                     LNQGQLVFEGHPRDINDMKFKEIYGRDAERVG"
     gene            15134..16975
                     /locus_tag="PLE39_07635"
     CDS             15134..16975
                     /locus_tag="PLE39_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNE68235.1"
                     /translation="MKEKNSPASAWRSVQIGLAVLLGLFVYAYGFDVTKVNLEELRSE
                     RRQESLTRVTRTLAQPDIIGYDQEKAIVNLPVFVPCPSDDLLATLPEADTSGAYLTVS
                     PACAAPGESVQVEGFNFVAHTEGPIRFVPGNDPANVVELGRETVETDGSGHFSTSFVM
                     PERLSDAPQFIRVTISQNVGAPHFTQNAKDTWDKIIETVFMALLATTAGTILAFPISF
                     LAARNLMKSVRSPLTSVSLSVLGWPIGMGLGYFIVNLVGKLAIPLSENIAISLVSVVA
                     TPFIFWYAMRWALPQEETKIPELAMRLTRMLVIFLAILVAFFGLFQLAGFTTSLSLAI
                     EESLGVLGFLATFLFQVSDIVRISIPALGALAAGGALSNFLGRIGQHTAEKSPAARVK
                     SINFIVAALAGATIFGLSGWLVEWLYQIGRVELTFWGPIIIGALLGLLLAFFTKPKDT
                     LPTGMVIYSITRTILNALRSVEALVMAIVFVIAVGIGPFAGMMALGLHTIVSLAKLYS
                     EQVESIMPGPLEAITATGANRLQTIIYAVIPQIVPPYISYTMYRWDINVRMSTIIGFV
                     GGGGIGFLLQQNINLLNYRAASTQMLAIAIVVASMDYISSVLREKYV"
     gene            17119..18609
                     /locus_tag="PLE39_07640"
     CDS             17119..18609
                     /locus_tag="PLE39_07640"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNE68236.1"
                     /translation="MKKNKRTPQSRLKSAFLAGLFILAACAQPTPAPTETSAPIADPA
                     PYSATIFEVNSDAKASPSRLADERPNIIFVLTDDQPYETIQYMPTVRDVLLKNGVNFE
                     NGFVTTPLCCPSRSSMLTGQYAHNHQVLTNRMPLGGAPKLDDSSTIATWMNDAGYRTG
                     YFGKYLNSYEDLKPYGYVPPGWDEWGTLLDAAFYNYNLSVNGTVKAYGETPEEFCADV
                     TTRNAVDFITASKDEPFFMVVGYFNPHSPYFWAERHDEQFRANSALKAEPYRNPAFME
                     EDRSDKPKYLQDLNSIAVSKIDITYKQILRSLLSVDDGVASILNALDKTGLSEKTIIV
                     YLSDNGLTVGDHGFGLTKNCAYESCIRVPFIMYAPGMFPARTESRFVANIDLAPTFAD
                     LAGFAAPDTVDGTSLLPLLKNESITWRDDLLFEHWPTEDGVGADIPEFYAVRNAEWKY
                     VEYSTGEKELYDLVNDPYELNNLAGQPEYAEVQTQLQQRLIELKKE"
     gene            complement(18791..19801)
                     /locus_tag="PLE39_07645"
     CDS             complement(18791..19801)
                     /locus_tag="PLE39_07645"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNE68237.1"
                     /translation="MSIGKIKWQSIVSQYAYPETWRSLWQVVNSVIPFFVLWYLAYRS
                     LEIGYWLTLLLTIPAAGFMVRMFIIFHDCCHGSFFETPLANNRLGLLLGVAVWTPYYA
                     WKHSHAIHHATAGDLDRRGIGDVYTMTVEEYLASPWYKKFGYRVMRNPFILFTIGALI
                     VFVFAHRFWEKGAGKREKNSVVWTNIALLGFVGWTIYHIGWQAYLLVEIPILLMACGA
                     GVWLFYVQHNFEPTYWERHQNWEFFKAGMDGSSFYKLPKVLQWFTGNIGFHHIHHLSP
                     KIPNYKLEKCHNENPIFQIQPLTFRDSLKSLYFRLWDEKEKMLVGWRAIKKYKMKMQM
                     DN"
     gene            complement(19886..21124)
                     /locus_tag="PLE39_07650"
     CDS             complement(19886..21124)
                     /locus_tag="PLE39_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68238.1"
                     /translation="MSSFQKKIGLIAVLAFALAGLLFFRPSRTTPPADSPTVMVVDVT
                     ATSTWTPSRIPENIPIADDPLKESILAAGDYLVRQQLTNGELSYQVDFISGERSFSPS
                     HVRLISGTGALFTVCRVSGDSKYCDAADLALDHYLELLVTDPAHFKGTCLFTEGTCQL
                     GGAAMTVDAIYKRWQASGELILHERNLLETAINLGYFIVSMRRPEGGFYHAYDPYFGG
                     IVDDDFYLSYFPGQSLVALTQLYEITGNDFWLEQAREVNDFMITQPISEDHWHSYGLS
                     MLARWDALTEADLTYAKQIADVVIAGEVRSLNLKNTSMATATKIEALSALAQAFYLSN
                     TDHAKLEPEIRAFITFVQARQLPDNNCGWTISEEITEKFGGGFFSNCEDTTIRVDGQQ
                     NWINGVTAYLEYRSMIKARK"
     gene            complement(21136..21807)
                     /locus_tag="PLE39_07655"
     CDS             complement(21136..21807)
                     /locus_tag="PLE39_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNE68239.1"
                     /translation="MLKALIFDFDGLILDTETPEVLVWQSIYRDHGFELPVDEWEKTI
                     GGYGLSNFDAAEHLALLSSGRLDSVSMRSRYRKEADAIIHASPILPGVVGLIEQAREQ
                     GLQVAIGSSSPHSWVDTHAKRLGIFHYFKYIICQDDVAPGRTKPNPDIYLKALEVLKV
                     QNTEAVVFEDSPNGVLAARRAGIFAVAVPNPLTKRLGVSGDLTVPSLAGISLHEISQA
                     ISLRS"
     gene            complement(21809..22129)
                     /locus_tag="PLE39_07660"
     CDS             complement(21809..22129)
                     /locus_tag="PLE39_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68240.1"
                     /translation="MSNWATYDGGRSIGKTSPAGDVILYDEEHHLGARITLRRGKNFI
                     SVSCNFYGWMDHTRFFNALSEAEREYRAMKTAIAAMMEPLKAAASNKLKGWEAISEFV
                     RRFP"
     gene            complement(22122..23039)
                     /locus_tag="PLE39_07665"
     CDS             complement(22122..23039)
                     /locus_tag="PLE39_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68241.1"
                     /translation="MSIRPLDLLDLPVITRYRNNVLTLDSARALTRGHPLGAMGLLAY
                     INPARHLYAAIANGGDTALLGGVIHTRGETFAKLLYLAPSSNLGDPTLPALIEHLAAQ
                     AGDWKAFHVLAEVDESSDVFPALRTAGFSVYAWQRLWDVSLLEKTESDISLRWRRTQS
                     LDLPAVQSLHYQIVPPLMHPVEPAPKRVLGFVCQNLKCYASVTAGMYGIVLTPLIHPE
                     EQNVGEKLLALVAGLPDRRGRKVYLNVRSYEAWLEPVLADLGAQASSRQAVMVKHLAR
                     LIKEGQSVRTVPTNVGVPASRISRMDFDE"
     gene            complement(23084..23662)
                     /gene="pdxT"
                     /locus_tag="PLE39_07670"
     CDS             complement(23084..23662)
                     /gene="pdxT"
                     /locus_tag="PLE39_07670"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HNE68242.1"
                     /translation="MKIGVLALQGDFAEHISMLKKIGVDTAEVRLPKHLDGLDGLIMP
                     GGESTTFGKLAVAYGLIDPIRQFGKSHAVWGTCAGAIFLSKDIGRDQPLLGLMDIKVQ
                     RNAFGRQVDSFETDLEIDELMKATGTEHPYHAVFIRAPIIESVHGNARVLSALEDGRI
                     VAAQEGHLLATSFHPELTDDTRFHEYFISLAS"
     gene            complement(23709..24596)
                     /gene="pdxS"
                     /locus_tag="PLE39_07675"
     CDS             complement(23709..24596)
                     /gene="pdxS"
                     /locus_tag="PLE39_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNE68243.1"
                     /translation="MESKTGTWTEKKGLAQMLKGGVIMDVVNAEQAKIAEDAGACAVM
                     ALERVPADIRADGGVARMSDPDMILKIMDAVSIPVMAKCRIGHFVEAQILESIGIDYI
                     DESEVLTPADEEHHILKHNFKVPFVCGCRNIGEALRRIGEGAAMIRTKGEAGTGDVVE
                     AVRHARSVIGSIKQLQVMGDEELMTFAKNNGAPYELVKEVKELGRLPVVNFAAGGVAT
                     PADAALMMQLGVDGVFVGSGIFKSGDPAKRAAAIVKSVTHYQDASILAEVSKNLGEAM
                     VGRNAAQMPESEKLAVRGW"
     gene            24934..25362
                     /locus_tag="PLE39_07680"
     CDS             24934..25362
                     /locus_tag="PLE39_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNE68244.1"
                     /translation="MTEKKLTLRLDFHSGLPIYTQIVNQIQSQLANGILMPGDQLPTV
                     RALAQELRVNFNTVARAYRILDEARVISTQQGRGTYITEIPPPKVSEKLRSESLATLT
                     QRFISEAIRLGFSEREISQMVRDNLKSMKETLEQQKENVK"
     gene            25359..26300
                     /locus_tag="PLE39_07685"
     CDS             25359..26300
                     /locus_tag="PLE39_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNE68245.1"
                     /translation="MNTSLFKSLLEKNKQTKEDQHISPIAITIFTVFLLVAVFGAVIG
                     DGRFSDILIGGWVLGWTMIGAYFLFALRVASQWEKAVVLRLGKFHGLKGPGLFWLIPI
                     IDNATMWIDHRVMVTSFSAEKTLTKDTVPVDVDAVLFWVVWDAEKAALEVKDYQVAIS
                     WAAQTALREIIGQMSLADILVGRAKMDEELQKIIDERTTPWGVTVQSVEIRDVVIPQV
                     LEDAMSRQAQAERERQARVILGESEQQIAISFAEASQAYINNPTALHLRAMNMLFEGL
                     KEKGALVIVPSSAVDSMNLGGLSGMVSLAQNNQVKEK"
     gene            26336..26986
                     /locus_tag="PLE39_07690"
     CDS             26336..26986
                     /locus_tag="PLE39_07690"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNE68246.1"
                     /translation="MNTKTTSIIALGMIVIAIMAGVFLWNQLPEQMASHWNANDEVDG
                     TMPRFWGVFLMPIVTLGMFALFIFIPNMDPLKANIVEFRETFNLFIVFFVMFMLYVHT
                     LTLIWSLGYNNFKMSTTLLPIIGLMFIFIGYMLRKARRNFFIGIRTPWTLSSDTVWSK
                     THQLGSILFMLSGVFAMIGGFFGGMLAFWLMFVPLIGSTLFLVVYSYVLYRNEIKA"
     gene            27089..27916
                     /locus_tag="PLE39_07695"
     CDS             27089..27916
                     /locus_tag="PLE39_07695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HNE68247.1"
                     /translation="MFISYSILSYISMILLPIVAGFLIARNFNFGFKDFRKLFTTGAL
                     TFIAAQMLHIPVVYGLTALFSSGILPAPDKSITLIFNAIVLGLLAGIFEETARYILFR
                     TIRKSMDTWQTSIVIGLGHGGIEAILLGIISISTMLQMIAMRNPINLSALNLPADQLD
                     IVQQQIAAYWAQPDAMPLFGFMERISALSLHIGLSILVLYSIISNQKKWFWIALLWHA
                     FIDALAVYVFPKITAGENVITGVFLFELLILILGGGVLIYAIRFRTSFPAKNEAPTN"
     gene            complement(27997..29130)
                     /gene="dnaN"
                     /locus_tag="PLE39_07700"
     CDS             complement(27997..29130)
                     /gene="dnaN"
                     /locus_tag="PLE39_07700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNE68248.1"
                     /translation="MKVTVLQENLARGLSTVSRAVSPRSTLPVLSNILIASDEGRLRL
                     SATNLELGITCWIAARIDEEGSTTVPARTFADLVNTLPGDQVQLNLDVKTQNLHVKSG
                     SSNNDIKCIDAQEFPPLPVPEMDGAVQLNVADFKEMIQQVVFAASTDEARPVLMGVLM
                     HVEKDKLTMAAADGFRLSVRKAVLSNPVPQPLNLIIPSRALSELARVASDPEEPIYMV
                     APKNRGQVLFRVKDVEVVSQLIDGTFPDYQQIIPRSYKSRTLVSTASLLKACKQAEIF
                     AREGSNVARFDIKQSNGEMQPSEVEISATSEETGKNETIVEATVDGGSVLIAFNVKFL
                     REALEVIKTPNVALETSAANAPGVIKPVGDDNFLHVIMPMHLG"
     gene            complement(29201..31126)
                     /locus_tag="PLE39_07705"
     CDS             complement(29201..31126)
                     /locus_tag="PLE39_07705"
                     /inference="COORDINATES: protein motif:HMM:NF039189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HNE68249.1"
                     /translation="MKTVRRLYFYAVALISFEVVLWGLVGLIRSIVNPNLITDNAQAL
                     ATALSLILVGIPFFLIHWLWAQRVSARDEEEKTATVRAVFLYGALFATLLPAVQNLLA
                     LINRTFIGAARISYERALVGGTQSWPDNLIAIILNLLMAAYFWSILRAEWPTLPDVEN
                     FKDIRRVYRYLWMLYGLLMVVFGTQQILQFILQIPTDLIGTSGRETFVNATALLVVGT
                     PIWFFAWRFIQDALPDPDESGSVLRLVILYLLSIGGTITVLAAGGNILYDLLSRLFGE
                     NISNTDLLQNISDPLSLVIPFGVLWGYFGGQLNRQFDAETNIPRRSGMKRIYFYILSI
                     IGLGATVTGVLSLFVVIVNQLSGKDVDVIAADSVLKQLASAIAVIVVGLPLWLKAWNP
                     MQAEALADGESGGHARRSITRKSYLYLVLFASVLGTMAPAGMIIFLLIQASLKGGVEN
                     DFLNSILNAFQALIVFAVVLTYHLLALRKDGAAQADVLEEKQSEFRVVVFDHDGKFGE
                     ALKAVFTRHAPKLPVTVLMVGQPIPVDLKANAVVLSGSLAVNLPDHVEAWFRSFNGSR
                     LIVNDDAAGIYWMNDFGQAALSAQALAEGQEIRPRSAVKSTPIWTYVAYVFAALFACQ
                     LIFILLSLGISLVVGGF"
     gene            complement(31149..31622)
                     /locus_tag="PLE39_07710"
     CDS             complement(31149..31622)
                     /locus_tag="PLE39_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68250.1"
                     /translation="MKQDRFLTGILAGIGVLILLALVLFFVRQDDTKEYRVDASPEAT
                     VYNYVLAITNDDYQKAYSYLAEKEHKPSYEQFRQSFFNGNVSPDNVGVDVGTAEISED
                     DALVNLSLVYSPNDPFFGGYSSTDRAQLIRQNGEWKLTYMPYNFWSYDWYQEQFK"
     gene            31734..>33223
                     /locus_tag="PLE39_07715"
     CDS             31734..>33223
                     /locus_tag="PLE39_07715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNE68251.1"
                     /translation="MTISAPHWDLTNVYPSLESKEFKAAVDDYKKQVASFGKFFKNKL
                     GKVTPKTKAREIAPLVGEAIDRINSIQTLSSTIFPYIYSFVTTNSRDKVAMKTLSEME
                     QASLPMSQLYTQFTAWVGANAKKLGEIIKLDPSAKAHAFMLKESAQQSKYLMSEIEEQ
                     LAAEMSLSGGNAFGKLQGAVVSQMSVEFELNGKVEKLPMPALINLRSHPDESVRRRGY
                     EAENQAWEGVKETLAACLNGVKGEVIVLNKRRGRKDALHSAIEAARLDRKTLEAMLEA
                     MKDSFPMFRKYFKAKAKKIGKEQLAWWDIFAPLGKTDKVYSYEEARDFILENFEKFSP
                     DLSTFAKRAFDNNWIDAEQREGKRGGAFCTEVQALKESRILCNFDGSFDQISTLAHEL
                     GHAFHNECAYQADKTVLQQLTPMTLAETASTMCETIVTEAVLSTTKDPQEELAVLEAQ
                     IQGASQVVVDIYSRFLFEKEVFERREKSELSADDFNDIMERAQKAT"
BASE COUNT         8418 a         8804 c         8091 g         7910 t
ORIGIN      
        1 gaacttcgca tccgtgcggt gcttgaagtg ccaatgctgt atggcggcga gctgatcggt
       61 gtgctgaccg ccgacgagat caatgactcg acacgggtat ttaccgaatc cgatgaacgc
      121 ctgcttacct tgttcgcatc acaagcggcc ggagcaattc attcggcacg tttacgtgag
      181 caaaccgcac aacgtgtgaa tcaactgcaa gccttgcata cgatcgaccg tgcgatctca
      241 tcatcctttg acctgcgcct catcttgaat acggtcatct cccaatccat atctcaattg
      301 ggtgtggatg cggcagatat tttgcttttc aacccctacc tgcaaacact ggaatatgta
      361 gccgggcagg ggttccgcac gcgcaacatt gaacaaagcc gcctgcgcct cggggaagga
      421 cagggcgggc aggtcgcgct gaatcgtaca gttttgcatg tatcaaattt ggtcaaagca
      481 gggaccgaat ttacacgcgc ctcacttttg gttggagagg gttttctgga atactacggc
      541 gtaccgctca ttgccaaagg tgagatcaaa ggggtactcg aagtttttca ccgcacccca
      601 ctgcatgcaa gttccgattg gctgggcttc ctcgaaaccc ttgccgggca ggccgccatc
      661 accatcgacc agacccaact cttcgatgac ctgcaacgcg ccaaccttga attgatcatc
      721 gcatacgaca cgaccatcga aggctggtca catgccatgg acctgcgcga tgaagagacc
      781 gaaggccata cacgccgtgt caccgaaatg acccttgccc ttgccaacgc catggggatc
      841 agcgatgacc aaatgctcca cattcggcgc ggcgcactgc tgcacgatat cggcaaaatg
      901 ggcatcccgg acaagatcct gctgaaagag gaaaaactga ccgaggccga atggaaggtc
      961 atgcgccagc accccaacta tgcgcgagac atgctgcaac ccatcaagta tctgcaaaaa
     1021 tcgctcgata tcccctactg ccaccacgaa aaatgggacg gcagcggtta tccacgcgga
     1081 ttaaaggggg aacagattcc gcttgtggca cgcatctttg ccatcattga tgtatgggat
     1141 gccgtcacca ttgaccgccc ctatcgcaaa gcgtggacaa aacaaaaagc cctcaaatac
     1201 atccgtgaac aaagcggaaa acatttcgac ccttccgtgg tcgccgcatt tctaaagatg
     1261 ttcgataaaa aagacccgcc cgccggcaag cccgcaaaac gaaaaaacaa atagcagtgg
     1321 cacaaaaaaa ccgcacctca atttaacaaa ggacaccaga ctcacagttc tggatggatt
     1381 acaattgcgc ccatgattta ccgacacttt aaacatgaaa cttggttgta tgttctggtg
     1441 ctgttgattg cggtgtcagt ccgcaccatt caacttggcg ccatgcctct ctcagattca
     1501 gaagccgcgc ccgccctgca ggcattgcaa atcacccagg ggcagcgccc cttgctcagc
     1561 ccgcatccct tttacatcct ttccactgcg gttttatttt tcctttatgg cgaggggacg
     1621 aatttcctcg cccgattttc cccggcctta atcggcagcc tcctcgtcct cattccgctt
     1681 ctcttcaccg aacggatcaa accccgcccg ggcctgatcc tggccctttt cattgcgctt
     1741 gacccgggca tggttgccct gtcacggcag gcggcgagtc cgatctttgc gatcaccttc
     1801 ctgctttttg ccattggatt ttatgaaaac aacaacttga aacttgcggg cagctttgcc
     1861 gcccttgccc tgcttggcgg accatccatc tggctggggc ttctcagcat tggcattgca
     1921 tgggcgatct atcggggcgt gcaattgcgt ggttccgaag agcaggcctc ggttaatgtc
     1981 aaagaagcgc tttggccgtt cgtcgtcact ttggttgtgg cgggcacgtt tttctttctc
     2041 atccccaatg ggctcagcgc cgcattttca tccattcccg aattcttcaa acgctggaca
     2101 atcgcttctg attttcccgc ctcacgtttt ctgatcgcgc tggccgtgta tcaaccgctg
     2161 gggattctgc ttgctgtcat tgccctcatc cgtggatggc agcatggaag ccgccgcatc
     2221 atttcactga gtctgtggtt cattgtggcg ttgctgctgg ctattttcat cccatcgcgc
     2281 gagatcgccg atatatcctg ggcgctcatt ccgctgtggg ggctggctgc acttgaactc
     2341 atccgcaacc tgcatatctt cccggaagag cgcaatgaga ttttcggtgt ggttttcctc
     2401 acggctttca tatgggtatt tgcctggctc gatttctccg gcatggtctg gcttcccaat
     2461 accacccgcg aatatatgat gcgcttctgg cttttggtag gcgcactgct cctgatcgcc
     2521 atcagtcttc tgctggttgc ggcgggttgg tcaattcgca cggcgcgtat cggcggggtc
     2581 ctgggcatgg cactggtcct tggcgcactc ggcctgggcg gaacttttgg tgcggcaggt
     2641 ttacgaggtt tggataaccc tgagatctgg tggcaggcgc cgctgccagt tcaagccaag
     2701 ttgattcaag caagcgtcaa cgacctttct gagtggggca ccggcaacga caatatggcc
     2761 cccgtgatca tcgccgggtt ggattctccc gctctcgaat gggccttgcg cgaacatcaa
     2821 gtggaggttg ttgaaacgct tgatatctcc agtgcgccct attttgtgat taccccgctt
     2881 caaaacgacc ccgcctttgc ctccacctat cgggggcagg atttcacctg gagaaaaacc
     2941 ttcctgtgga atgagaccct gccgcaggat tggatccgct gggtgactct gcgcgaagtt
     3001 ccgcaaaccg gcgaatccat cattctctgg gctcgtgatg atcttttcct cgacaaggcc
     3061 aatcaatcca tcccctaaat gaatggaaaa aaatgacaaa taaacccact ccatccatca
     3121 ttgcacttga cggccctgcc gcatcgggca aatcaaccct cggaaaaaga ctcgccgatg
     3181 cactcgggtt cctgttcttt gataccggta tcatgtatcg cgccatcacc tggatcgcac
     3241 tacaacatga catggacctg cgcaatgaag ccgcggtctc tcaacttgcg cagaatgccc
     3301 agatcgacat ccgcccgccc tccaaaaatg atggacgtgc ctgcgatgtg atcattggtg
     3361 aaaaagatgt tacctgggat atgcgcggcg gggatgtgga tgcgaatgtt tctgtggttt
     3421 ccgcctatgc caaagtgcgt caggcgttat cggagcaaca acgccgcatc ggcatgcgcg
     3481 gcaacgtggt gatggtcggc agggatattg gcacggtcgt cctgccaaat gccgatctca
     3541 aaatttatct cgatgccagt gcagaagaac gcgcccgccg ccgatacaac gagatcattg
     3601 cccgcggaga acccgccaac ctcgatgaaa ttctcaaaaa ggtgatcgaa cgcgatcgca
     3661 tcgactccac gcgtgcggtt gcccctctcc gccccgccga tgatgccatc ctcatcgact
     3721 cggatgcaat ggacgcagaa caggtttttg catacgtgtt gaatttatgc aagtaaaaga
     3781 ctaccgcatt ccatttcaca ttcaggccaa ccggttgtgg ttgcggtttg gcattcgcac
     3841 attgttcaac attttgggga gtgtaaagat cacaggggcg gaaaatattc cgcttggcag
     3901 cccgtacgtg atcgcaatga accatatttc gatttttgac ccgccgctcg tggttagttt
     3961 ctggccggaa gaaccggagg tcattggcgc atcagatgtg tttgcaaaaa aagggcaggg
     4021 gaccatcctc tcgttatatg gtgtcatccc tgtccatcgc ggcacgtatg atcgcaccct
     4081 gcttgaaaag atcatcttaa ttctgaaaca aggccgtcca ctggtcattg cgcctgaagg
     4141 cgggcgttcg catgaacccg ccatgcgccg ggcaatgccg ggcgttggtt atatcatcga
     4201 gcacgccggg gtaccggttg tgccggtggg tctggtgggg acaaccgatg atttctggca
     4261 gcgttcaaaa cagtattggc gcgggcaggg agaaaaaccc cgcctcgaaa tgcgcatcgg
     4321 aaaaccgatc cactttcagc cgatccttga aaagggcgcg gaacgccgcg aagcccgcca
     4381 gaaaaatgca gacctggtga tgcaacatgt cgccgggctg ctgcccgcgg aatatcatgg
     4441 agtttatacc gggcaggcca tttccacata attatggata ccccgactcc caaaccagtc
     4501 agtgaagtat ggcggcctga gttggttcgt ttgccaaaaa tgacccatgc ccgccgcgca
     4561 tttcgggtct ttgggcacgg attgacaaaa ctggtggcca aactctgcct gcatgtaaca
     4621 gtggaaggct tggagaattt tccgcgcaag gcgggagcag agcctgtttt ggtggtcatc
     4681 aaccatctcg gcgactcaga tgtcccggca ttgatctcgt cactgccatt tccgcctgat
     4741 gcattggcca aagtcgagct gtacgacctc cccatcctcg gaaaattaat ggactggtat
     4801 ggtgttattt ggctgcatcg cggccgcccc gataaacgcg ccttgcgtgc cgcattggat
     4861 ggtctggcgg aagggcgggt gattgttatt gcgcctgagg ggcggtactc gctcaccggg
     4921 gcattggaag aggggaacaa cggtgcggca ttcctggcct acaaatcggg ggcggcggtt
     4981 ttgccgattg ctgtaacagg aactgaaaat gagaccgtgt atgggaattt gaagaaattc
     5041 cgccgtgcgc gggtgaatct gcgggtgggg aaaatgttca agttggctgg gcaggcccag
     5101 ggcggagaaa gaggcggggc cggggagcgg caggaggtca tcgcgcaggg gacacgtcag
     5161 atcatggagg cgctggcggg gctgcttcca gaaaaatacc gcggcgcgta ttcaggctga
     5221 ttttagggaa attgggcggt gccccccaca atcttgtaat taaaattgta attatttggg
     5281 gctataattc agaaaatggg atcactttcc acgctgccgg caagcatcgc attccccgcc
     5341 cctcccactc ttgcaggatg ggcagtatgg ttgtctttgc tgggaatgct ggcttatgcc
     5401 ctgtaccatt ggcgtgccta ccagcccgca atgaaacgaa ttgaatgggg atttttagtt
     5461 ggccttttac tcctgacact tgttgcaaac gtttttatat taagaccttt tgcttcttca
     5521 gcacgccctt tacccaactt gccggttcaa ccgccgggct cggcgctgat cgttttttca
     5581 gccattccat ggttgttgag cggcgggttg atcgggccgc ttggtgcggc ggcggtcggt
     5641 gctttggctg gcctcctgcg cggcacactt gatacctatt cgcttttttc catcattgaa
     5701 tttgcgttga tgggagcgtg gttttccacg aatacccgtc agcgtttccg cacacctgtg
     5761 tatagcctgt tacgacaacc cattgtcggc gctttgttgt tgattccgct gcatgcgttg
     5821 ttctacattg tggtggcatt gttcacccaa tggggtgtgg atatccccac cacggcgcgg
     5881 cttgattttg ccatcagcaa cacaggtatt gtgaccctcg catttggaat tgaaatgctg
     5941 gtgggcggca ttgtggcaca gatcatttcc atgtcctttc ctgcggtttg gggtggaaaa
     6001 cagcaggcct tgcagccctc gcctggcgag aaaagccttg agtcgcgttt tcaatttgca
     6061 gtaggcacgt ttatttccct tttgttgtta acccttctga tcggtgattg ggtggtggcg
     6121 ggtcaggccg ctcgtgacat gttgcaagac cgccttacca gtgcgggtga agcagcggcc
     6181 caaagcattc cttttttcct tgaaaccgga cagaacctgg cggtgcagct cgctgccgat
     6241 ccgcgcctgt tggaagccac cggtgatgaa ttgcattcca tcattggctc gcgtattcag
     6301 gccgtgccat attttgacca gttcttcgta ctggaagcca atagtgaaac cctgctggca
     6361 gggtacccgg atgactctgt taccaccttc aaattgtatc ccgatgaaag catggggttg
     6421 attctggcgg ccaacggggt cttaacccag atctattcca tcccaccgat ctccgctgat
     6481 gaatcggcac gggtttcgtt cctcgtcggt attgtagata agttcggttc ggtacaacgg
     6541 gtattgatcg gccgcaccac cctgaaaacg aatccgttga ctctgccttt gatcgaaggc
     6601 attgacaaca tgaaaaacct caacggcgca ggatttctgc tcgatgagag caaccgcatc
     6661 atttatcact ctgaaaccat tgaaatggct gccacataca acggtcagcg cagcactgaa
     6721 gccgttttct acgacgacac cgcggcagac ggaacccgac aactggtcta ttatcaaccg
     6781 gtgacgggcc gcccctgggc agttgtgtta accgtgcccg cgcaacaggc ccaacaattg
     6841 gcattaaaca tcgccatgcc gctttcattg atgatccttg tcctggcggt cgttgcgatg
     6901 atttctttac gggtgggctt gcgtgtggtg acagggtcac tgcaaagcct cgccaccgaa
     6961 gccaaccgca ttgctcaggg caacttcgac catccgcttc aaacagaagg cgtggatgag
     7021 gtggggcagt tgcgtcgtgc cttcgaaaag atgcgttcca gcttgcaggc acgcctcaac
     7081 gaaatcaaca gcctgctccg tgtaagtcag ggtgttgcct ccagtttgga aatgcaggat
     7141 gcggtcaagc cggtgttgga cgccatcctt gccacgggtg caaactcggt cagtgtggtg
     7201 ctttcaccaa gtattttgcc cgacacgttt gtagaattgc cctcacggtt tgctgtgggc
     7261 actgtgcagg acatttacgc ccatcttgat gaccagatcc tcgcgctggc gcaaagccag
     7321 gagaaggtgg tgcttccaaa cctttctcgc agccgcgaac tggaactcga cccatccctg
     7381 ccgcagccgc tctccctgct ggcggtggcc ttgcgccatg aaaaccgtta ctacggtgtg
     7441 gtatgggcag ggtttgaaca accgcgcatg ttctccgatt cagatatccg ttttatgacc
     7501 acgctcgctg gtcaggcggc actggcagtt gccaatgccc acctcttcct gaatgtggaa
     7561 gcctcgcgcc gtcagttgaa agccattctc aattccacac cggacccggt gctggtgacc
     7621 gatcaccgca accggttgct gcttaccaac cgtgccgccg ccatagctct gggacaatct
     7681 gaagacacca ccagtgatat cggtcgcaag accgaacagg tcgtcaagat cagaccgctg
     7741 cttgccctgc tggaaagcgc gaccacagaa aatcaatcca ctgagatcac tctggctgac
     7801 aaacgcacct atctggctac cgcatcggct gtaatggtcg aaggcaaaca gatcgggcgt
     7861 gtgtgcatca tgcgcgatgt gacccacttc aaggaactgg atatgatgaa gtcggagttc
     7921 gtcgccacgg taagccatga cctgcgttcc ccgcttacgc tgatgcgcgg gtatgccacc
     7981 atgctcgaaa tggtcggtga attgaatgaa caacagcagg gatatgtcaa aaagatcatt
     8041 tccggcgtgg agaacatgtc gcgccttgtc aacaatctgc tcgacctcgg gcgcattgac
     8101 cttggggttg gcttgcaagt ggaaaatgtc agcgtgctcg atattatcga acgtgtgacc
     8161 ggtgcgcttc agctacaagc cagtcaaaag aatatccagt tgaccgtgga actttccaaa
     8221 gatatgccgc atgccatcga agcggatcag gcccttctgc atcaggctgt gtataacctt
     8281 gtggaaaacg ccatcaaata cacacccaat gaccaacgtg tcaccattcg caccgaaacg
     8341 cagccgggca gcctgacctt cattgtcgaa gattcaggca tcggcattgc cgccgacgac
     8401 ctgccgcgcc tgttcgaaaa attctaccgc ggcaagcagc gtgaagcgcg cgctcaacat
     8461 ggatccgggc tggggctggc cattgtccat tccattgcca ccaatcacgg cggcaaggtc
     8521 tgggtggata gcgtggtggg taagggcagc actttctatt tacagatccc gctcacccag
     8581 ccgaaggaaa acaaacgcat tggtccgtag gaactgaaaa aaattaagca gcaaagggcg
     8641 taaagaggtt tctctttacg ccctttgctg cttaattccc tgcatcatcc tttataatga
     8701 cactggaaag tgaatacaaa aatgaaactc attcgaattt ccctcttcac cctcatgatc
     8761 gcgttgtcgg catttcagct tgcagccgca cagggtgatg acccattggc cgtagtgatg
     8821 accgccgaag ggaccatcat gccgcccatg ttggaatact tcaagcgcgg cattgagact
     8881 gccgaaaaca gcaatgcaga cgtactcgtc attcaactga atacccccgg cggaagcatt
     8941 gaaaccatgc tggatatcat tcaaaccatg cgcgccagca aggtacccat cgttgtgtat
     9001 gtttcgccaa acgatgccat ggcaggcagc gcaggagcat tgatcaccat ggcggggcat
     9061 gcctctgcca tggcccccaa gacagccatt ggcgcagcca gccccattga tagttccggt
     9121 gcagacattg aatccacgct ggaaacaaag atcaaggaaa tactgaaggc caaggcccgt
     9181 gttctggtgg aagcacgcgg agaagaagcc acccgcctcg cagaagccat gatcgatgat
     9241 gccaaagctg tcacagccag tgaagcgctt gaagcaaatt tgatcgattt cgtttccgac
     9301 gacacggaag acctgctgca agccttgaat ggtttcaccg tcaaaatgga cgatggcagt
     9361 gaggtcacgc tcaacactga aaacatccgc acggaagaac ttggcatcag tttcatcgaa
     9421 cagctattaa tgatgctcac cgacccgaac attgccttta tcctgcttgc cattggtgta
     9481 cagggaattc tgatcgaaat ttccagcccg ggcggctggt tcgcaggctt ccttggtgca
     9541 gtctgtctta cgctggctgt gtatggaatg ggtgtactca cggtcaactg gttcggtttt
     9601 atctttttgg tcattgcctt tgtgctgttc atccttgata tcaaggccgc cgcacacggc
     9661 gcgttgacca cggccggtgt ggcatccttc atcgtcgggg cgctggtgct gttcaattcg
     9721 ccgggtacac cgcaatttca acgcgtatct gttccgcttg tgatcggtgt gggtgttttc
     9781 atcggcgcat tgttctttgc gatcgtgctg gttgcattgc gctcgcgcca taatccgatc
     9841 cagaccgggg ttgagtcatt gcttgggaaa acaggaacag ccaaaacgtt cgaagaagaa
     9901 gcggggcagg ttcaagtggg aagcgaacta tggagcgcag aaaaagcccc cgggtcagaa
     9961 tccattcgta agggtgaccc ggtcgaagtt gtcgaagtac gcggattaag attaatcgtg
    10021 aaaaaaaagt aatgcaacat taatgttgcg ccacaagagg accttatgcc tgccacacct
    10081 acgagagaca gagtcaaacc cgaaacagaa gcacgcccat tgcgccgccc cgaatggatc
    10141 aaggtgcgcg cgccttcggg cgaaaccttt gaacgcctgc aagtgctgat gcgctcgaaa
    10201 gaattgcaca ccgtttgtga agaagccatg tgcccgaacc tcggcgaatg ctggggcagc
    10261 ggcacggcta catttttgat gcttggcgat gtatgcacac gcacctgtgc gttttgcgat
    10321 gtaaaacatg gcaagccgtt gttgatggat tggggggagg ccgaacgtgt agcgcaggcc
    10381 gtgaaatcaa tgaacctgaa acatgcggtg atcacctcgg tgaaccgcga tgaacgccgc
    10441 gatggcggtg cgccgatctt tgcgatggtc attcgcagaa ttcgtgaggt gctgcccggc
    10501 tgttccatcg aagtgttgat ccccgatttc aaagggagca tcgaagcgtt gaagatcgtc
    10561 atggatgccc gccctgaaat tttgaatcac aatgtggaaa ccgttccgcg tttgttcaag
    10621 accgtgcagc cgcaggataa ttatgagtgg gctgcggcaa ctttatcgaa cgccaagaaa
    10681 ctcgaccccg atgtgctgac caaaagcggc atcatgctcg gcctcggtga aaccatggac
    10741 gaagtgaagc aggtgatgcg cgatcaacgc agttggggcg tggatattct caccatcggc
    10801 cagtatttgc agccaagcaa gaaacacatg cccatggacc gttattacac gatggaagaa
    10861 ttcgccgaac tcaaagcctt cggcatggag atcggtttca agtgggtcga gtcgaatccg
    10921 ctggtgcggt cttcatatca tgcagcagaa caggtgcggg cgttaagcat cgtgcatcgt
    10981 aagttgtacg gagaaaaagc ataaccagtg gttagattaa gcccctgtga cgcaggggct
    11041 ttttttcctt attgaggcag tttaacccgt aacttttcag ggtttaaaac gactagaaat
    11101 aaataagata tccatgcagg catgccgcca aaaacattaa agttgtgtcc attcttaatt
    11161 tctggcagta gaagcgacgg cgggtaacac aaagtaaaaa tatttcacca aggagacaca
    11221 attttatgta cacaacccca aaacacattt tccttcgcag cctgcttctg gcggcctata
    11281 tcctggcgct ggtgatacag ccattttcag cccgtccggc ttttgcagcc ccatctcttc
    11341 aaacaataaa ctatcaaccc accctcactg ccacccccgc cagcggaccg gcacccctca
    11401 ccgttaactt ttcaagatac gtcgccacgg atccgaacgg cccacttgtc agctacgtca
    11461 tgttggattt cggtgcaggc cccggcctgg gcgctgaaat tgactttggt gacggtacat
    11521 caaccttcat ccttgataaa acttcaagca tggtatacaa cctgcctggt acttacaccg
    11581 ctgttatgac agtgttctac aacaattcca ccagcattac caccacgctg atcatcactg
    11641 tcgaccccag tgtaaccccg gaacagcttc ccctaattgt tgcctcccct gccagtggaa
    11701 ctgcgccatt ggtcgtcaac ctcaccagtt ctgccactat gaatccgaat ggtcccctca
    11761 tcaacaacta caaagtggat tatggagacg gcacaagtga cctttatttc ttcctcgatg
    11821 gcgccccatc atttatcaat aatggaagcc acacctacaa cactccaggc acatatacag
    11881 ccacactgac agtaacctac aataacgcaa ccagtgccag tgccacgacc acgatcgtcg
    11941 tgaatcctgt gctcagcaat gtggtgcgaa gtacggcggt caacctttca gctactttga
    12001 aaaagaccac ggtcaatgtg agtggaaccg ttatagttca aaacgagaat ggcacagtta
    12061 ttcccggtgc tatcgtctca ataacctggg tcaagccaaa tgggcgggtg gtcacacaaa
    12121 atgcaaaaac aaatgcaaac ggtgtcgcca aattcagcac cagtggcaat cgcggcacct
    12181 acacactgac tgttaccaat atccgcaaaa caggctatag ttttgacagc atcaacagtg
    12241 tggtgagcgg aagcattaca aagtaagcac tggaattaaa aataacaaca aaaaggccat
    12301 ccgtcttgtc agcggaatgg cttttttgtt gtcaaacatt tggattagtt gtatagttga
    12361 aaccaccaca caaatttcat gaaaaaaggc tttatcttta tgaacattct cgaaactccc
    12421 cgcttaattc tccgcaatgc aaacgcagac gatgcgcctt tcatgcttga tctgttaaac
    12481 cagccctcat tcatccgttt catcggggac cggggcatcc gcagcctcga tggtgcgcgt
    12541 gaatatattg aaaccaggct ggttaaaagt tacaaagaca atggctttgg gttgtatatc
    12601 accctcttga aggaagagaa tcttcccatc ggcctgtgtg gacttgtaaa aagagatgga
    12661 ttggatgaca ttgatatcgg ctttgccttc ctcgcccaac acgaatcaaa ggggtatgga
    12721 tttgaatccg cttctgcggt catggaatac agcaaaaaca cgctggggtt gaagcgaatc
    12781 gcaggcatca ccaccccaga taatgaaaaa tcgatcggtc tgctcgaaaa actgggactt
    12841 aaattcgaga aaatgatcaa acttccaaac gatacagagg aattaaagtt attcatccgc
    12901 actttgtgac gtgaaaaata caggaaagta gccagtgaaa aacataatct ttttttaaga
    12961 caagcgtact tgtttgtaca accgttatga actattatct ttaagtcagg ggtatagtgc
    13021 cccataaact ttttatctct taagaggaga agataatgaa caaacgtatc gcttttgtcc
    13081 tgctgacgct cgtcgttgcc atatcaatga tgctgtcagc ttgtggccct gccgccactg
    13141 aagcccccgt tgccaccgaa gcccctactg aggctcctac cgaagccccc accgaggctc
    13201 ctgtcgctac tgaaccgccc atgcccgcca ttggctcccc tgaacacccc atcaaagtgt
    13261 tgttcgtccc ctctgtagat gcgaacatca ttgtgaccgg cggtgaagtg atggctgctg
    13321 ctttgaacga agcgaccggc ctcacctttg aagtggtcgt cccgacctcc tatgccgcaa
    13381 ccatcgaaga aatgtgcgcc tccccgtcag ataccatggc cttcatccct ggccttggct
    13441 atgccattgc cagccagttg tgcggcgtgg atgtttcctt caaggccatc cgcttcggct
    13501 tcccggtcta ctgggctgaa tacattgtag cccgcgatag caaataccag actctggccg
    13561 acctcgaagg cgccaagtgg ggttacggtg accagggttc aacctctggc tacatggtcc
    13621 ctaccgttga actcgccgcc gctggcatca cccccggcga acaggttcag accggcggtc
    13681 acaaccagac cgtcaccgct gtgtacaacg gcgaagttga ttttggtacc gtcttctaca
    13741 gcgttccgct gacccctgaa ggcgctccac tcttcagcta cgatgatttc gtcgctggca
    13801 agatcaccag cctcgagcag tatgagatcc ccactgaatc cgttcccaac tgtgctccgg
    13861 ataccgaagg caagaaattg ctttgcgacg gctggcgcat tcttgatgcc cgtgccaaca
    13921 tccgcaccga agccccggac gtgatccaga aggttcgcat tctcggcgtc tctgccgcga
    13981 tcccgaacga caccctgtcc ttcggccccg aattccccgc tgacctgcgc acccagatcg
    14041 aagaagctct gctcgccttc gccgcaaccg aagcctgggg cacctccatt ggttccgcag
    14101 atttctacgg ctggtctggc attgccccgg ccaccgatgc cgagtatgac atggtccgcg
    14161 ccatggttgc cgccaccggc tacgaacccc ccaagtaaga tccgcttttc ataaattttc
    14221 gggcagggag ccattccctg cccgaacttg ttttatatac ccgtacagca attgtgatcc
    14281 aagtttttat ttgagctgtt attttcagct tatatcttat aatctggtga aacgcatcaa
    14341 gcacaaacat cttttcaaaa gactgtgaga agccacaatg ctccaggtaa aaaacctaac
    14401 caaaatatac gacgggggcg tcaaagcgct ggacaatgtc tcttttgaag tacccaaggg
    14461 ccagtttttg gccgtgatcg ggttgagcgg ctccggcaaa tccacactgt tgcgctgcat
    14521 caatcgtttg atagacccca ccgaaggcca gattttatgg gatggtgaag atgtcaccgc
    14581 tgccaccccg gaagaaatgc gcctcattcg ccgcaagatc ggcatggtct ttcagcattt
    14641 caacctcgtg catcgctcga aggtcatcac caatgtgctg gccggacggt tgggctacgt
    14701 caacccagcc tggagtttcc tcaaccgctt tccaaaagaa gacatcgaaa aagccatcaa
    14761 acaactggag cgggtgggca ttgcagataa ggccgaccaa cgcgccgacg aactcagcgg
    14821 cggccagcag cagcgtgtgg gaatcgcacg cgcgctcatg caggacccga acatgatcct
    14881 cgccgatgaa ccggttgcca gccttgaccc ggtcctcgcg cacagcatca tgaaacacct
    14941 tgaagagatc aacaaaaaag atggtgtaac cgtgctatgc agcctgcact tcctcgatct
    15001 cgttcatcgc tatgctgacc gtgtcattgc gctcaaccag ggtcaactgg tattcgaagg
    15061 acaccccaga gacatcaatg acatgaagtt caaagagatc tatggccgcg atgcagaaag
    15121 ggtgggataa cccatgaaag aaaaaaattc acccgcatct gcatggagat cggttcaaat
    15181 cgggctggcg gttttgcttg gacttttcgt ttacgcctac ggcttcgatg tcaccaaggt
    15241 caaccttgaa gaattgcgca gtgaacgcag gcaggaaagc ctcacccgcg tcacccgcac
    15301 actggctcaa cccgatatca tcggctatga ccaggaaaag gccattgtca acctgccggt
    15361 gtttgttccc tgcccgtcag atgacctgct ggccaccctg cccgaagcag atacatccgg
    15421 tgcatacctt acggtcagcc cggcatgtgc ggcacccggt gaatctgtgc aggtggaagg
    15481 cttcaatttt gttgcccata ccgaaggtcc gattcgattc gtgccgggca atgatcccgc
    15541 caacgtggtg gaactcggcc gcgaaacagt tgaaaccgac ggctccggac atttctccac
    15601 ttcattcgtc atgcctgagc gtcttagcga tgcgccgcaa ttcatccgcg tgaccatcag
    15661 tcagaacgtg ggcgcaccac acttcaccca aaacgcaaaa gacacctggg ataagatcat
    15721 tgaaacggtg ttcatggcct tgctggccac gaccgctggc accatccttg ccttccccat
    15781 cagttttctg gctgcccgca acctgatgaa atctgtgcgc agtccgctca ccagtgtttc
    15841 tctttccgtg ctgggctggc ccattggcat ggggctggga tatttcatcg tcaatcttgt
    15901 gggcaagctt gccattccac tttcggaaaa catcgcgatc agtttggtca gtgtcgtggc
    15961 aacgcctttc attttttggt atgcaatgcg ttgggccttg ccgcaggaag aaacaaaaat
    16021 ccccgaactt gccatgcggc tgacccgtat gctggttatc ttccttgcga ttctggtggc
    16081 cttctttggt cttttccaac tggctgggtt cacaaccagt ctcagccttg ccatcgaaga
    16141 atcgcttggc gtgcttggtt ttctggctac cttccttttt caggtcagtg acatcgtgcg
    16201 catttcaatt ccagctttgg gtgcattggc agcaggcggc gcgctttcca acttcctggg
    16261 ccgcatcggg cagcacactg ccgagaaaag ccccgctgca cgtgtgaaga gcatcaattt
    16321 catcgtagcc gcgctggcag gtgcaaccat ttttggtttg agcggctggc tggtggaatg
    16381 gctgtatcaa atcggccgtg ttgaattaac tttttggggg ccgatcatca tcggcgcact
    16441 gcttggcttg ttgcttgcct tcttcaccaa acccaaagac accctgccca ccggcatggt
    16501 catttacagc atcacccgca ccatcctcaa tgcattgcgc tctgtggaag ccctcgtcat
    16561 ggcgatcgta ttcgtcattg ccgtcggcat tggtccgttc gcaggcatga tggcactcgg
    16621 cctgcatacc atcgtcagcc ttgcaaaact atattcggaa caggtcgaaa gcatcatgcc
    16681 tggtccgctc gaagccatta cagccacagg tgcgaatcgt ctgcaaacca ttatctatgc
    16741 agtcattccg cagatcgtcc cgccatacat ttcctacacc atgtatcgct gggatatcaa
    16801 cgtgcgtatg tccaccatca tcggtttcgt gggcggcggc ggcatcggct tcctgcttca
    16861 gcaaaatatc aacctgctta actaccgcgc cgccagcacc caaatgctcg ccatcgccat
    16921 tgttgtagca agcatggatt acatttcatc tgtgttgcgc gaaaaatacg tgtaaatcga
    16981 cacactcatg caaaacagcg cagacaagtg cagtctgcgc tgttttttat tacgatataa
    17041 tgactcgcgc ttcacgcttt gccgaggcct gcttcgcctc ccgatcattc tctccctgca
    17101 acagtaccct gactaaaaat gaaaaaaaac aaacgcaccc cacaaagtag attgaaatcc
    17161 gcattccttg cgggcttatt catcctcgca gcctgcgcgc aaccaacccc ggcgcccaca
    17221 gaaacatccg ctccaattgc tgaccctgcc ccttattcag cgaccatttt cgaggttaat
    17281 tcagatgcga aggcttctcc atcgcggctt gcagacgaac gtccgaacat catcttcgtg
    17341 ctgaccgatg accagccgta tgaaaccatt caatacatgc cgaccgtgcg cgatgtactt
    17401 ttgaagaatg gtgtgaattt tgaaaatgga tttgtcacca cgccattgtg ctgcccaagc
    17461 cggtcgagca tgttgacggg gcaatatgcg cacaaccatc aggtgttgac caaccgcatg
    17521 ccgctcggcg gcgcgcccaa actggatgac tcttccacca tcgcaacctg gatgaacgat
    17581 gcaggctatc gaaccgggta ttttggaaaa tatctaaact cgtatgaaga cctcaagccc
    17641 tacggctatg taccgccggg ctgggatgaa tggggaactc tgcttgacgc ggctttttat
    17701 aattacaatc tttcagtcaa tggaaccgtg aaagcctatg gcgaaacccc ggaagaattt
    17761 tgtgccgatg tgaccacgcg caacgcggtt gatttcatca cggcttcaaa agacgagcca
    17821 ttcttcatgg tggtgggata tttcaacccg cactcgccgt atttttgggc agagcggcac
    17881 gacgagcaat tccgcgcaaa ctcggctttg aaggcggaac cgtatcgcaa ccctgcattc
    17941 atggaggaag accgcagcga caagccaaag tatttgcagg atttgaattc gatcgcagtc
    18001 agcaaaatag acatcaccta caaacagatc ctgcgttccc tgctgtcggt ggatgatggt
    18061 gtcgcatcca ttttgaatgc actcgataaa acagggctga gcgagaaaac catcatcgtt
    18121 tatctttcag acaatggatt gaccgtggga gatcatggct tcggtttgac aaagaattgc
    18181 gcatacgaaa gctgcatccg tgtgccgttc atcatgtacg caccgggcat gttccctgca
    18241 cgcaccgagt cacgttttgt cgccaatatt gaccttgccc ccaccttcgc cgatctggca
    18301 ggctttgccg cgcccgatac agtagatggc accagcctgc ttcctttgct aaaaaatgaa
    18361 agcatcacct ggcgcgatga cctgctcttc gaacactggc ccacagaaga tggagtcggt
    18421 gcggatatcc ccgaatttta tgcagtgcgc aatgccgaat ggaaatacgt ggaatattcc
    18481 acaggtgaaa aggaactcta cgacctggtg aatgacccgt acgagttgaa caatctggcg
    18541 ggtcagcctg aatatgcgga agtccaaacg caactgcaac aacggctgat cgaattgaaa
    18601 aaagagtaat tatgcaagta gtcaaataag ttaaaagata cggacgattt ataaaaaagc
    18661 cgaccctgta atggatcggc ttttttcgtg taaattcgcg aaatccgcgg ctaaagaatt
    18721 gaaagactaa attcggggtg tttcatattt catttatggg cttctcccaa ctctgagaca
    18781 agctcgggaa tcaattatcc atctgcattt tcattttata ttttttgatc gcgcgccagc
    18841 cgacgagcat tttttccttt tcatcccaca aacggaagta cagcgatttc aagctgtctc
    18901 taaacgtaag cggttgaatt tggaagatcg ggttctcgtt gtgacatttc tccaatttgt
    18961 aattggggat cttcgggctg aggtggtgaa tatgatggaa accaatattg ccggtaaacc
    19021 attgcaacac tttgggaagt ttatagaacg aactgccgtc catgcccgcc ttgaagaact
    19081 cccaattctg gtggcgctcc cagtaggtcg gctcaaagtt atgctgaaca tagaataacc
    19141 agaccccggc gccacaggcc atcagcaaaa tgggaatttc aaccagcaag taagcctgcc
    19201 agccgatgtg gtagatcgtc cagccgacaa aaccgagcag ggcaatgttc gtccaaacga
    19261 cgctgttctt ttcacgcttg cctgcacctt tttcccagaa acgatgtgca aacacaaaaa
    19321 cgatcaacgc gccgatggta aacaaaatga acggattacg catcacccga tacccgaatt
    19381 tcttgtacca gggcgaggca aggtactctt ccacggtcat ggtgtagacg tcgcctatcc
    19441 cacggcggtc aaggtcaccg gcggtggcgt ggtgaatggc atgggaatgt ttccaggcat
    19501 agtaaggggt ccacacggca acaccaagca gcagccccaa acggttgttt gcaagcggcg
    19561 tttcaaagaa cgagccgtgg cagcaatcgt ggaagatgat gaacatccgc accatgaacc
    19621 cggcggcagg gattgtgagc aacaaggtta gccaatatcc aatttcaagg ctgcgatatg
    19681 ccagatacca caacacgaaa aagggaataa ccgaattgac cacctgccac aaactacgcc
    19741 aggtttcagg atatgcatat tgcgaaacga tggattgcca ctttattttt ccaatactca
    19801 tgggaatctc cttctttcat aagattattt ttcaggtctg ccgaattagc gggagcaaag
    19861 caggaactct gctttgctct ctttattatt tcctggcttt aatcatactc cgatattcaa
    19921 gatatgccgt aaccccgttg atccagtttt gctgcccatc aacacggatg gtggtatctt
    19981 cacaattgct aaaaaatccc ccgccaaatt tttcggttat ttcttctgag atggtccaac
    20041 cacaattatt gtctggaagc tgacgggcct gcacaaatgt gataaaggcg cggatctcag
    20101 gctccagctt cgcatggtct gtgtttgaaa gataaaaagc ctgcgccaga gccgaaagtg
    20161 cttcgatctt tgttgcggtg gccatgctgg tgttcttcaa attaagtgaa cgcacttccc
    20221 cggcaatgac cacatcggcg atctgtttgg catacgtcag gtcggcttca gtcagcgcat
    20281 cccaacgagc cagcatggat agaccgtaac tgtgccagtg gtcttcagag atgggctgtg
    20341 tgatcatgaa atcgttcacc tcacgggcct gctccaacca aaaatcattc cccgtgatct
    20401 cataaagctg ggtcaacgca accagactct gcccgggaaa ataggacagg taaaaatcgt
    20461 catccacaat gccgccaaaa tatgggtcat atgcatggta aaacccgcct tccggtctgc
    20521 gcatggaaac gatgaaatac ccaaggttga tcgcggtttc aagcaaattg cgttcgtgca
    20581 aaatgagttc gccgctggcc tgccagcgtt tataaatggc atccacggtc atggctgcgc
    20641 cgcccagttg acaggtgcct tccgtgaata aacaggttcc tttgaaatgt gcgggatcgg
    20701 ttaccagcaa ctcaagataa tggtcgagcg caaggtcggc ggcatcgcag tatttcgaat
    20761 cgcctgaaac cctgcacaca gtaaaaagcg cccctgttcc cgaaatcaga cgaacatgtg
    20821 agggagaaaa agaccgttcc cctgaaataa aatccacctg atacgacaac tcgccattgg
    20881 tcaattgctg gcgcacaagg taatctcccg ccgcaagaat ggactctttc aatgggtcat
    20941 ctgcaatggg gatattttct ggaatgcgcg acggggtcca ggtggaggtt gcggtcacat
    21001 caacaaccat cacagtggga gagtcagcag gcggggttgt acggctgggc ctgaaaaata
    21061 acaacccagc caatgcaaat gcaagcacgg cgatcaaccc gatctttttc tgaaatgacg
    21121 acatggtctt tgacttcagg atctcaatga gatcgcttgc gaaatttcat gcagggatat
    21181 cccagccagc gacggaacgg tcaggtcgcc ggaaacgccg agtcgttttg tcagcgggtt
    21241 tggaacagcc actgcaaaaa tgcctgcccg ccgcgcggcg aggacaccat ttggagaatc
    21301 ttcaaaaact actgcttcgg tattttgaac cttgagcact tccaatgcct ttaaataaat
    21361 atccgggttg ggtttggtgc ggccgggggc tacatcatcc tgacaaatga tgtatttgaa
    21421 ataatgaaag ataccgagtc ttttcgcatg tgtatccacc catgaatgcg gcgaactgga
    21481 accaatggca acctgcaagc cctgttcacg ggcttgttcg atcaaaccaa ccaccccggg
    21541 caaaatgggg ctggcatgga tgatcgcgtc tgcttctttt cggtagcggc tgcgcattga
    21601 aacagaatcc agccttcccg aagaaagaag cgcaagatgt tccgccgcat caaagtttga
    21661 gaggccgtat ccgccaattg ttttttccca ttcatccaca ggtaattcaa aaccgtggtc
    21721 acgataaatg gactgccaca ccaatacctc aggggtttcg gtatcaagaa tcagtccgtc
    21781 aaaatcaaag atcaaagctt tcaacatttt atggaaaccg ccttacaaat tctgagatcg
    21841 cttcccaccc cttgagcttg ttcgaggcgg cggctttcaa gggttccatc atcgcggcga
    21901 tcgccgtttt catggctctg tattcacgct cagcctcgga aagcgcattg aagaaacgtg
    21961 tgtgatccat ccacccgtaa aaattacagg agacggaaat aaaattcttg ccgcgcctca
    22021 atgtgatgcg cgcaccaaga tggtgctctt cgtcatagag gatgacatcg ccagccgggc
    22081 ttgttttgcc aatgctgcga ccaccatcat aggttgccca attactcatc aaaatccatt
    22141 ctactaatcc gcgaagcagg cacgcccaca ttggttggaa ccgtacgcac agactggcct
    22201 tccttgatca atcgcgccag atgtttcacc atcaccgcct gacgcgatga cgcctgcgcg
    22261 ccaagatctg ccagcacagg ctcaagccac gcttcatacg aacgcacatt taaataaact
    22321 ttgcggccac ggcgatctgg cagccctgcc accaatgcca ataatttctc gccgacattt
    22381 tgctcttcag gatggataag cggggtcagc acaattccat acatgcccgc agttacactg
    22441 gcataacatt tcaaattctg gcatacaaac ccaagcaccc gtttcggggc aggttccaca
    22501 ggatgcatca acggcggcac gatctgataa tgcagacttt ggactgcggg caggtccagc
    22561 gattgagtgc gcctccacct taagctgata tctgattctg ttttctcaag caggctcaca
    22621 tcccaaagtc tttgccaggc atagacggaa aacccggcag tccgcaatgc cgggaaaaca
    22681 tcacttgatt catccacctc agccaataca tgaaatgcct tccaatcgcc agcctgggcg
    22741 gccaaatgct caattaacgc aggcagggtt gggtccccca aattggaaga aggggcgaga
    22801 tacaacagct ttgcaaatgt ttcaccacgt gtgtgaatta cgccgcccaa aagggccgta
    22861 tctccgccat ttgcgattgc cgcatataaa tgacgggcag ggttgatata agccagcagc
    22921 cccatcgccc caagtggatg tccacgcgtg agggcacgcg cgctatcgag agtcaagaca
    22981 ttatttcgat atcgcgtaat aacaggcaga tcaagcaggt caagtgggcg gatggacatt
    23041 ttttatagtg caggggcaca gtgaaatact gtgcccctgc aatttacgat gccagagaaa
    23101 taaaatactc gtgaaaacgt gtgtcgtcgg tcaattcagg atgaaaggaa gtagccaaca
    23161 aatgaccttc ctgagctgca acaatgcggc catcttccag ggccgaaagc acccgtgcat
    23221 tcccatgtac cgattcgatg atgggagccc gtataaatac ggcatgatat ggatgttctg
    23281 tgccagtggc tttcatcaac tcatcaatct caaggtcggt ttcaaaggaa tcgacctgcc
    23341 tgccaaatgc atttcgctgg accttgatat ccatcaatcc gagcaaaggc tggtcacggc
    23401 caatatcttt tgaaagaaaa atggcaccgg cacaggtgcc ccaaacggca tggctttttc
    23461 caaattgtcg aatggggtca atcaaaccat aggcaacagc cagctttcca aatgttgtcg
    23521 attcgccacc gggcatgatc aacccatcga gcccatccag atgttttggc aggcgtactt
    23581 cagccgtatc cacaccgatc tttttcagca tggaaatatg ttctgcaaaa tcgccctgca
    23641 gggcgagaac tccgattttc attttcaaat tccgtaggag catggcggcg ccatgctcct
    23701 acaatagttt accaaccacg aacagcgagc ttttcgcttt caggcatttg agcggcgttg
    23761 cgtcccacca tggcttcgcc gagattcttg ctcacttccg caaggatgga tgcatcttga
    23821 tagtgcgtca ctgatttaac aatggcggca gcccgtttgg cagggtcacc actcttgaag
    23881 ataccagaac ctacaaacac accatccaca ccgagctgca tcatcaaagc cgcatcagca
    23941 ggagttgcta ctccaccggc agcaaaatta accacaggca ggcgtccgag ttctttcact
    24001 tcctttacca gttcatacgg tgcgccgtta ttcttcgcaa aggtcatcaa ttcttcatcg
    24061 cccatcacct gtaattgctt gatggagccg atcacgctac gggcatgacg aaccgcttca
    24121 accacgtcac ctgtaccagc ttcgcctttg gtacggatca tagccgcgcc ttcgccaata
    24181 cgacgcaaag cctcgcctat attacggcat ccacaaacaa acggaacttt gaagttatgc
    24241 ttcaaaatgt gatgttcttc atctgcaggc gtaagaacct cagattcatc gatgtagtca
    24301 atgccaatcg attccagaat ctgtgcttct acaaaatgac cgatacggca ttttgccatg
    24361 acagggatgc ttacagcgtc catgatcttc aaaatcatgt ccgggtcaga catgcgggca
    24421 acgccaccgt ctgcacgaat atcagcaggg acacgttcca acgccattac tgcgcatgcg
    24481 cccgcatctt ctgcaatctt ggcctgctcg gcattcacca catccatgat gacgccgcct
    24541 tttaacattt gggccagccc ctttttctca gtccatgtac cagtttttga ttccattttt
    24601 atacactcct ttaattaatg cgtatgaatt ctaccatgca gccatattta aatcataaga
    24661 gcactgtaaa catattttcc aatccctgga ctcgtgaaaa tataaattca agtaatgtat
    24721 tgcctgcggc ataaacattg aaagggacat gttgtgacca attccaacct ttaccacatt
    24781 cagtggcatg aaccatcttc gctggacatc aaagtaaaca ttcccagatc catggtattc
    24841 cactggattc cattaaaagg gaagggcttg acttaaactc aaaaatagtt acaattatta
    24901 caatgataca attagtacaa ttaggataac cgaatgacag aaaagaaact caccctgcgt
    24961 cttgatttcc attcaggctt gcccatttac acccaaatcg tcaatcagat acaaagccag
    25021 ctagccaatg gaatattgat gcctggagat caactgccta ccgttcgagc actagctcag
    25081 gaattacgcg tcaatttcaa cactgtagca cgtgcttatc gaatattgga tgaagccaga
    25141 gttatatcca cacagcaagg aagaggaacg tatattaccg agataccacc gccaaaggta
    25201 agtgaaaagt tgcggagtga gtcgctggca acgttgacac aacgatttat cagcgaagcc
    25261 attcggctgg gattctcaga gcgagagatc agtcaaatgg tgagagacaa tttgaagtcc
    25321 atgaaagaaa cgcttgaaca acagaaggag aatgtcaaat gaatacaagt ctatttaagt
    25381 ctttacttga aaagaacaag caaactaaag aagatcaaca tatttcgcca atcgccataa
    25441 ccatatttac agtattcctt ctggttgctg tatttggcgc agtcattggt gatggtcgtt
    25501 tttcagatat tctgattgga ggctgggtac tgggatggac catgatcggc gcatatttcc
    25561 tttttgcatt gagggtggca tcgcaatggg aaaaagctgt tgtactgcgc ctgggaaaat
    25621 tccatggact gaaaggccca ggtttgtttt ggttaattcc gattattgac aacgcgacca
    25681 tgtggatcga ccatcgtgta atggtcacat ctttttcagc ggaaaaaacc ctgaccaaag
    25741 ataccgtacc tgtagatgtg gacgctgtcc tgttttgggt ggtgtgggat gcagaaaaag
    25801 ctgcgcttga agtaaaggat taccaggttg cgatctcatg ggccgctcaa actgctttgc
    25861 gcgagattat cgggcaaatg tcacttgctg atattctggt gggccgtgcc aaaatggatg
    25921 aagaattaca aaaaattatt gacgagcgaa ctacgccttg gggcgtaacc gtacaatctg
    25981 tggagatccg tgacgtggtc attcctcagg ttttagagga tgccatgtca cgtcaagcac
    26041 aggcagaacg ggaaagacag gcacgcgtca ttcttggaga atcggagcag caaattgcaa
    26101 tttcatttgc agaggcatca caagcctata tcaacaatcc aacagcactg catttgcggg
    26161 caatgaatat gctgtttgaa ggcctgaaag aaaaaggcgc actcgtaatc gttccatcat
    26221 ccgcagtgga ttcgatgaac cttggcggct taagtggaat ggtgtcgctt gcacagaata
    26281 atcaggttaa ggaaaaataa tcgtttcacg tgaaacgaag tagaaaagag aaaaaatgaa
    26341 taccaaaaca acttcaataa ttgcacttgg catgatcgtt attgccatta tggctggtgt
    26401 attcctttgg aatcaactcc ccgagcaaat ggcttctcac tggaacgcca atgatgaagt
    26461 ggatgggacc atgccaagat tttggggagt atttctgatg cccatcgtca cactgggaat
    26521 gttcgcgctt ttcatattca ttcccaacat ggatccgctc aaggccaata ttgtggaatt
    26581 ccgcgaaacg ttcaacctgt ttattgtttt ctttgtgatg ttcatgctct atgtccacac
    26641 actaactttg atctggagtc ttggctataa caacttcaaa atgagcacaa ccttactgcc
    26701 aattatcggc ctgatgttca tctttattgg ttacatgttg agaaaagcca ggcggaactt
    26761 cttcatcgga attcgaactc catggactct ttccagtgac acagtctgga gtaaaacgca
    26821 tcaactcggt tcaatcttat tcatgctttc aggagtgttt gccatgatcg gaggattttt
    26881 tggcggcatg ctggcattct ggctaatgtt cgttccgttg atagggtcaa cactttttct
    26941 agtagtctac tcatatgttt tgtatcgcaa tgaaataaag gcttaaacaa aaagatgcgg
    27001 cttcagttta gaagccgcat ctttttgttt cacgtgaaac taccgagcaa atactcactt
    27061 aacaaaaact atacagatat ggagaaaaat gttcatctca tattccatcc tgagttacat
    27121 aagcatgatt ttgttgccca ttgttgctgg cttcttaatt gcgcgaaatt tcaactttgg
    27181 attcaaagac tttcgcaaat tatttacaac tggcgcgttg actttcattg cggcacagat
    27241 gcttcacata cccgttgtgt atggattgac cgccttgttt tcgagcggca tactcccagc
    27301 gccggataaa tccataacgc tgattttcaa cgcaattgta ttgggattgc tcgcaggtat
    27361 ttttgaagaa acagcccgtt acattttgtt tagaaccatc cgcaaatcaa tggacacttg
    27421 gcaaaccagt attgttatcg ggctaggaca tggcggcatc gaagcgatcc tgcttggtat
    27481 catttcgatc tcaacaatgc ttcaaatgat tgccatgcgc aacccaatca atctttctgc
    27541 tttgaattta ccagcagatc aacttgacat cgttcaacaa caaattgctg cttactgggc
    27601 acaacctgat gcaatgccat tgtttggatt tatggaacgt atctcagcat taagcttgca
    27661 cattggctta tccattcttg tgttgtatag cattatttcc aaccaaaaga aatggttttg
    27721 gatcgcattg ctctggcatg ctttcattga tgcattagcc gtttatgtat ttcccaaaat
    27781 cacagctgga gaaaatgtta ttacaggtgt gtttttgttc gaactcctga tcttgattct
    27841 aggtggtggg gtattgatct atgccattcg atttcgtacc agctttcccg caaaaaatga
    27901 agcgcctaca aattagaaca tttgttcttt tacttagcag tcaaaaaaag tccgctgaat
    27961 gttttattca gcggactttt tttgactata tcaacactat cctaaatgca ttggcatgat
    28021 cacatgcagg aaattatcgt ccccgactgg tttgatgaca ccaggtgcat tcgccgcaga
    28081 agtttccaat gcaacattcg gggtcttgat cacctctaaa gcctcgcgca aaaatttgac
    28141 attgaacgcg atcaacacac tgccgccgtc tacagttgcc tcaacaatgg tctcattctt
    28201 accggtttct tctgacgtgg cagagatctc aacttcactg ggctgcattt caccattcga
    28261 ctgcttgatg tcaaaacgag ctacgttcga gccttcacgc gcaaaaattt cagcctgctt
    28321 gcaagccttg agtaatgaag ccgtggagac caatgtccgc gatttgtagc tgcgcggaat
    28381 gatttgctga taatcaggga aggttccatc aatcaactgc gaaacaactt ccacgtcctt
    28441 tacacggaac aacacctgac cgcgattctt cggagcgacc atataaatgg gttcttcagg
    28501 gtctgaagcc acacgcgcca attcgctcaa agcgcgtgat ggaatgatca aattcaacgg
    28561 ctgcggcacc gggttcgaaa gcacggcctt acgcaccgaa agacggaaac cgtctgctgc
    28621 ggccatcgta agtttgtcct tctcaacatg catcaacaca cccattaaca caggacgggc
    28681 ctcatcagtg gatgcagcaa agacaacctg ctgaatcatt tccttgaaat ccgccacatt
    28741 caattgcaca gcaccatcca tttcgggcac gggcaatggg gggaactcct gggcatcaat
    28801 gcacttgata tcgttattgg aagatccgct cttaacatgc agattctgtg ttttcacatc
    28861 cagattaagc tgcacctgat cgccgggtag tgtattcacc aaatctgcaa aagtgcgggc
    28921 aggcaccgta gtggagcctt cctcatcaat acgggcggcg atccaacagg taatgcccaa
    28981 ctcaaggttg gtggcagaaa gccgcaaacg gccttcatca cttgcgatca aaatattgga
    29041 taaaacagga agggtgctgc gcggcgaaac ggcgcgggag acagtactca aaccgcgtgc
    29101 aaggttttct tgaaggacag ttactttcat aggttatcgc ctcggatgga tttttttgaa
    29161 agtatatcat taactcattg tcaaaaacac agcacgccga tcaaaagccg ccaaccacca
    29221 aagaaatacc caaagacaac aaaataaaga tcaactgaca ggcaaacaat gccgcgaaaa
    29281 cgtaagcaac atacgtccat atgggtgtcg acttcacagc cgaacgcggg cggatctcct
    29341 gcccttctgc caatgcctgt gcggatagag ccgcctgccc gaaatcattc atccaataaa
    29401 tgcccgccgc atcatcatta acgatgagcc tgctcccgtt gaatgaacga aaccacgctt
    29461 caacatggtc gggcaaattt acagccaaag aacctgataa aaccacggca ttcgccttca
    29521 aatcaacagg aattggctgc cccaccatca acaccgttac aggtaatttg ggagcatgcc
    29581 ttgtaaaaac cgccttgaga gcctccccaa attttccgtc atggtcaaat accaccacgc
    29641 gaaattcaga ttgcttctcc tccaatacat cagcctgtgc cgcaccatcc ttgcgtaatg
    29701 ccaaaagatg atacgtcaac accacggcaa acacgatcaa agcctgaaaa gcattcaaaa
    29761 tactgttcag aaaatcgttt tcaaccccgc cttttagcga tgcttgaatt aacaggaaaa
    29821 tgatcatccc agccggggcc atggttccaa gcacggaagc aaacaaaacc aggtacaaat
    29881 atgattttcg agtgatggaa cggcgcgcat gcccgccaga ttcaccgtcg gcaagtgcct
    29941 ctgcctgcat gggattccag gcttttaacc aaagaggcag ccctacaaca atcacagcaa
    30001 ttgccgatgc caattgcttc aaaacagaat ccgctgcaat gacatccaca tctttaccgc
    30061 tgagttgatt gacgatcacg acaaacaacg agagtacccc ggttacagtg gctcccagcc
    30121 cgataatgga aagaatataa aaatagattc gcttcatccc tgagcggcgc ggaatgtttg
    30181 tttcagcgtc aaattgtctg tttaattggc cgccaaaata gccccacaaa acgccgaagg
    30241 gaatcaccaa cgaaagcgga tcgctgatat tttgcagcag gtcggtgttt gaaatgtttt
    30301 caccgaaaag gcggctgagc aggtcgtaaa ggatgtttcc acctgctgcc agtacggtga
    30361 tggttccgcc gatggacaga aggtagagaa tgacaaggcg cagaacagag ccgctttcat
    30421 caggatcagg caaagcgtct tgtatgaagc gccaggcaaa gaaccagatc ggtgttccaa
    30481 ccaccagcaa tgccgttgcg ttgacaaacg tttcacgtcc ggaggtcccg atcaaatctg
    30541 ttgggatctg caaaatgaat tgcaaaattt gctgtgtacc aaaaacgacc atcaacaatc
    30601 cgtacagcat ccacaagtat cgataaacac ggcggatatc tttgaaattt tcaacatcgg
    30661 gcagggtggg ccattcggcg cggagaatgc tccagaaata agcagccatc aacaggttaa
    30721 ggatgatcgc aataaggtta tccggccagg attgggtgcc gccgaccagg gcacgttcgt
    30781 aggagatccg tgcggcgcca atgaacgtgc gattgatcag tgccaacagg ttttgaactg
    30841 caggcaggag tgtggcaaat aatgcaccat acaaaaaaac agcgcggact gtggcagttt
    30901 tctcctcttc gtcgcgcgct gaaacccgct gggcccacaa ccaatggata aggaaaaacg
    30961 gaatgcccac caaaatgaga gacagggctg tggccagcgc ctgtgcgttg tcggtgatca
    31021 ggttcgggtt gacgatggag cggatcaagc cgaccagccc ccacaacacc acctcaaaac
    31081 tgattagggc taccgcgtaa aaatagagtc ttctgactgt tttcatcttt cactccatcc
    31141 tgtatctgct acttgaattg ttcctgatac caatcgtaag accagaagtt gtatggcatg
    31201 taagttaatt tccattcacc attctggcgg ataagttggg cgcggtctgt actgctataa
    31261 ccgccgaaga atgggtcgtt gggggagtaa accaaggata gattcaccag ggcatcatct
    31321 tcactgatct cggctgttcc gacatccaca ccgacgttat ccggggaaac attgccatta
    31381 aaaaaggatt gccgaaattg ttcatagctg ggcttgtgtt ccttttccgc cagataggaa
    31441 taagcctttt ggtaatcgtc gttggtgatg gcgagaacat agttatagac ggttgcttca
    31501 ggagaagcat caaccctgta ttctttcgta tcgtcctgcc gtacaaagaa caaaacaagc
    31561 gcaagcagta tcaaaacccc aatgcctgcc agaatgccgg taagaaatcg atcctgtttc
    31621 ataagaacct ccgtgttaat ttcattgtat aggttcaaag atgaaaaggc aagtcaaaaa
    31681 gttccaatgg cgctttggta tactagccat atctggtaaa tcaaggagaa acaatgacca
    31741 tatctgcccc acactgggac ctcacaaatg tctatccttc gcttgagtcg aaggaattca
    31801 aagccgctgt tgatgattac aaaaaacagg tcgcatcttt tggaaagttt ttcaagaaca
    31861 agctcggcaa agtcactcca aaaacaaaag cacgggaaat agccccgctt gttggtgaag
    31921 ccattgaccg catcaacagc atccaaaccc tttcttccac gatcttccct tacatttatt
    31981 cattcgtcac caccaattcg cgtgacaagg tggccatgaa gaccctttct gaaatggagc
    32041 aggccagcct gcccatgagt caactgtaca cacagttcac cgcctgggtc ggggcaaatg
    32101 ccaaaaaatt gggtgagatc atcaagttgg atccttctgc aaaggcacac gccttcatgc
    32161 tgaaggaatc tgcacagcaa agcaaatacc tgatgagtga gatcgaagag cagcttgcgg
    32221 cagaaatgtc gctcagcggt gggaatgcct tcggaaaact acagggagcg gtcgtttcgc
    32281 aaatgtcagt ggaatttgaa ctcaatggca aagtggaaaa actgccaatg ccagccttaa
    32341 tcaacctgcg ttcccaccct gatgaatccg tccgccgccg cgggtatgaa gcggaaaatc
    32401 aggcttggga aggtgtaaaa gaaacgctgg cagcctgtct caatggagtt aaaggcgaag
    32461 ttatagtctt gaataaacgc cgcggacgca aggatgcact ccactcggcc attgaagccg
    32521 cccgcctcga ccgcaagacg ctcgaagcca tgctcgaagc aatgaaggat tccttcccga
    32581 tgttccgcaa gtacttcaaa gccaaggcca agaagatcgg aaaggaacaa ctcgcctggt
    32641 gggatatctt tgccccgctc ggaaagacag acaaggtgta ttcctacgaa gaggcgcgcg
    32701 atttcattct tgagaatttt gaaaaattct cgcccgattt gagcacattc gccaaacgcg
    32761 cattcgacaa caattggatc gacgccgaac aacgtgaagg caagcgcggc ggtgcattct
    32821 gcactgaagt gcaggcatta aaggaaagcc gcattctctg caattttgac ggttcgttcg
    32881 atcagatcag cacgttggcc cacgaattgg gacatgcctt ccacaacgaa tgcgcctatc
    32941 aggcagataa gacggtgctg caacaactca cgcccatgac cctggccgaa accgcctcaa
    33001 ccatgtgcga aacgatcgtc accgaggccg tactctccac aaccaaagac ccacaggaag
    33061 agttggccgt gcttgaagca cagattcaag gcgcaagcca ggtggtggtg gatatttatt
    33121 cgcgcttcct cttcgaaaag gaagtgttcg aacgccgcga aaaatcggaa ctctccgccg
    33181 acgatttcaa tgacatcatg gaacgtgccc aaaaagccac cta
//
LOCUS       DAOQJC010000038        32941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_590_length_32941_cov_9.072706, whole genome shotgun sequence.
ACCESSION   DAOQJC010000038 DAOQJC010000000
VERSION     DAOQJC010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 32941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32941
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_590_length_32941_cov_9.072706"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PLE39_07720"
     CDS             <1..589
                     /locus_tag="PLE39_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68252.1"
                     /translation="YCGDRERAKGFSSCKSKRISARKVNALVLDAILNRILTPHYFNA
                     LLDETKKLYVDTETIDAQLSQKRLDLSYTERAIKNLLELVEAFGVNESAKERLRQKEL
                     EKLNLMQEIKDIETRKRLIKIEVTPEALALVFEQWRKQIIDADKNNDVAAVRALLAGF
                     IDRVEIESPNVTIRYKYPIQSLIATSPDLLPVGAP"
     gene            complement(651..1142)
                     /locus_tag="PLE39_07725"
     CDS             complement(651..1142)
                     /locus_tag="PLE39_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68253.1"
                     /translation="MSMNKPVSASTPVDTAPYSAQNQNTPRVYVGLTLTFVGLFIFTI
                     GSKPDWFGWDRSAVVGFVQIAVFLVGLALICLGGYISLLTLWHGQERTILADIGTRLV
                     GTGYVIAVFAGLADVFGMGSQSFPKVPYFGPWQATGVLIGQGVIALGFLLFIPFFPAR
                     KKN"
     gene            complement(1139..2236)
                     /locus_tag="PLE39_07730"
     CDS             complement(1139..2236)
                     /locus_tag="PLE39_07730"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNE68254.1"
                     /translation="MPIIVDAHADIAWNMLKYGRDYTRSAVETRQIEAGSDVVKQNDD
                     TLLGWPEYQNGQVAVIFSTLYATPIRWSKSENKAQVYKTYDEAHQLYREQLLTYHRMT
                     DAMPDKFRLIASNRDLDLILENWQSLPATASSGHPVGMVVLMEGAEGIRTLSELEEWH
                     EMGVRLIGPAWVGTRYCGGWNEPGPLTPDGRKLLAAMADYNFILDLSHMDEQAAVEAL
                     DIYRGPIVGTHANCLSLMPNSPSNRHFSDRIIEGIIERDGVIGVVPFNGYLKVGWVAG
                     KSERSEISLDHVANHIDHICQIAGDSLHAGIGSDFDGGFGLQSVPPEIETVADLQKLS
                     ALLLARGYNDVDVSNILGGNWLNRLRRDLPS"
     gene            complement(2285..3433)
                     /locus_tag="PLE39_07735"
     CDS             complement(2285..3433)
                     /locus_tag="PLE39_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNE68255.1"
                     /translation="MESLLESESLSVELPQVGEIRKGMIASIGPSQILISVGAKSEGV
                     VAGRELEQLTAEERAALKVGQEVNVYVLNPEDQNGNVVLSFKRAQEEMSWENVEKMIA
                     DETVIDTKIVGFNKGGLIAAVGNLRGFVPSSQISAVRRAQSSGDTPEQRWQKMVGQAI
                     SVRIIEVDRERRRLILSERSTNTETRSSMKDRVISELEEGKVYTGKVTSLADFGAFVN
                     VSGADGLVHLSELSWDRLQHPKEILEVGQEVKVKVINIDRDKKRIGLSIRALQDDPWK
                     SRVEKFSVGQLVEGVITRLTKFGAFARLEGDIEGLIHISELSENRVEHPKEVLKEGEV
                     KTLRVIRIDGEQHRIGLSLRKVDSAAFADKDFKMLTQGFQDPDEENKE"
     gene            3648..4748
                     /locus_tag="PLE39_07740"
     CDS             3648..4748
                     /locus_tag="PLE39_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE68256.1"
                     /translation="MPAIFPFTAIVGQERMKRALILNAVDTRIGGVLIRGERGTAKST
                     ASRSLAALLPHVKIVQDCRFGCDPDKSSTWCTECKERYAASKKLPIAERATPFINLPV
                     SATEDRVVGTLDIEKAIQKGERHFEPGVLAGANRGLLYIDEVNLLDDHVVDILLDSAA
                     MGVNTVEREGISFAHPARFILVGTMNPEEGDLRPQLLDRFALSVDIVGIRDARERVTI
                     MERNLSYEKDSEAFRKQWLPKEEELSSKIARARELVDQVTHTSRDLLSIAALTASLNV
                     DGHRADLVILKAARAEAAFEGRTKINDHDIALAAELALPHRIKRTPFQQAELTTEQLQ
                     ERIEQLAGQSMPEDSDEKSESKQDGEGEEKKT"
     gene            4799..5710
                     /locus_tag="PLE39_07745"
     CDS             4799..5710
                     /locus_tag="PLE39_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNE68257.1"
                     /translation="MQQQEVFASSNANWWEGGQKVKAGETFQPRKLNTPLDKLTRKQA
                     GRRSLTKTERKHGRYIKARPAGDKLNDIAFDATFRAAAPFQKQRTEERKKLAFSIKKS
                     DLQRKIRVKRVANLVLFLVDASWSMAVAERMNATKGAILSLLTDAYQRRDRVGLIVFQ
                     KDRATLVLSPTNSVQLAQRALMDIPVGGKTPLSAGLFMAHDVLTHEKHTHPDVEPLLI
                     VLTDGAGNVSLGTLPPQEESHKFAELIANEKIRSVVINMEHAAFDQGLAQELANNLKA
                     PCYALSELKAESLYNTVIDEMSKPAKK"
     gene            complement(5982..7397)
                     /locus_tag="PLE39_07750"
     CDS             complement(5982..7397)
                     /locus_tag="PLE39_07750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE68258.1"
                     /translation="MIFSNTPRPETLKSLADVKHVPFWLDDPARPEPASALTESITAD
                     LVVIGAGFTGLWTALLAKQADPSREVVLLEAGETGIGASGRNGGFVASSLTHSFQNGL
                     NRWPKELKKIIELGHKNLDGIEATIKQFNIDCDFIRSGEINIANEEYQIEELREEAEH
                     SAEYGEHVQFLDREQIRARVNSPTYLAAIHDSSVAMVNPARLVWGLRRACLEVGVRLF
                     EGTQVTSLEEKRDSILVKTSYGQVESKRVALATNAFPPLLKRLSFYVVPVYDYVLMTE
                     PLSKEQRDAIGWHGREGVSDNGNQFHYYRTTADGRILWGGYDAIYYQNNGVAPHLENR
                     PETFARLAEHFFQTFPQLTGLRFTHAWGGVIDTCSRYTAFWGQAYGGKLAYAMGYTGL
                     GVGASRFGAQVMLDLLDGKKTERTELQMVKSKPFPFPPEPFRSPIVNFTRWSLNRADD
                     NGGQRNLWLKILDALGLGFDS"
     gene            complement(7508..8572)
                     /locus_tag="PLE39_07755"
     CDS             complement(7508..8572)
                     /locus_tag="PLE39_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE68259.1"
                     /translation="MKNQTFLITVFAAFVLSLGFLSVSDAFAHPSAQSEETPTPSSDR
                     PPVPAEIMEGVNNLRLSHGIAPLNAHPVLMQIGQMEADGIAAGREGHWRPEGITLGQW
                     LLSLGYPLSGDISLDGYRSENWGFAHNADEAVAMWLSDDEHENTMLSLERSDIGVGIA
                     KMPDEDVYVIVLETALQTKSGQMQYDAIVYLTGIPMTQAAYSSMATLAAENGLLPQNV
                     APVFLNTAMPDGNVYHEVKYGQTLWSIAISYGTTIKQIQQLNGLTDTNLRTGQKLLVL
                     KGATQPAPGGASTAPTLAQSPQTFPTPTMVPAKTTEPEPTPTLTPEQRRENMMGIAAI
                     GVAAIFLGGLFTAMTRKKPL"
     gene            complement(8592..10460)
                     /locus_tag="PLE39_07760"
     CDS             complement(8592..10460)
                     /locus_tag="PLE39_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68260.1"
                     /translation="MKRFLLGMLIFVLAACSGAVPTSAPAEPSFFEVTPAPADLKLDS
                     ASEEIQRALLESAAQWTTLQMDGTITTYLPDGTTQTFREQVWLEPLKSRFKVEVNGIT
                     DPAQHGLKLIDGVNLYDANLSLAQVQIAPVPDFIRVGQYIPPLVPGQVNTNPIWTQLG
                     TPLSQLAFASDFSQNGGIFKPLAAESIAGREALVVEWTYDANSLPSLKMWVDTQTGIL
                     LKLQEFTKGNSSQLAGERVVDHISFDVTFDETLFTPPAGLQQAGLPNAEAEAVPVVTA
                     SGSPSSEDAGELYFFLQPRQAGQSIQLARVSGICVFDSANCPPMQIVTVPFAFNFTIN
                     ALSWSPDGKLAAFSYSDSSNGTPTKLWIFDPAANTWTSIAQFAYIDPPFWSPDGTWIA
                     FRTLDGLGGEDVYVVRRDGSELKSVSLNLPVEGRPYIMDGWYTDHILMRSALPSNAGN
                     IFLVRASDGAAKPMFETQQSKSQFVASPDASLLAYDEIDYNTQGHVIKVMEPDGANAV
                     ALTSFAGGSIYPLVWSPDSSLIAFNHYSNSADGSPIAEVHVISRDGKTQSLVYTGTTI
                     GRLLFSPNGRTLLVEETTSISGGHLFLIDLATLEQKILQAPGLSTDFDWYAPSWRP"
     gene            complement(10563..12770)
                     /locus_tag="PLE39_07765"
     CDS             complement(10563..12770)
                     /locus_tag="PLE39_07765"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNE68261.1"
                     /translation="MKFTSKFAAVLLLLVFMFSVVPSAATQPAQAAACYWAQFIADVT
                     IPDGTNFAPGTAFKKTWRLKNIGTCAWNAGDVSLIFDSGEKMGAPASSALPTTVNPGQ
                     TVDITVDMVAPSAAGHYFGYYKFKSTQGGVFGIGSTANKSFWVEINVTSSSGTGYDFT
                     SNAASATWSSGAGTLTFPGTDGSANGFALKLDKPKFENGIESAQAGLLFVPNNVNNGY
                     IQASYPAFKVQSGDRFQATIGCEYGATSCYVAYRLDYQIGSNPVKTFWTFREKYEGLT
                     YNANLDLSSLAGQDVKFILVISAYGSQVGDRALWGNPVIARAGGTVATNTPTVTGTLP
                     TTVASKTPTPGPNACDRAQFIKDVTVADGTTFQPGTTFAKTWRIKNVGTCTWTNYSLM
                     FDSGEKMGGPDSALIPTTVAPGQTVDITINLTAPANAGTYRGYWKLKSSTGVPFGIGS
                     DGTKSFWVEIKVAGTPVTPSTPATPVTPGTPVTPATPISGTSYDFVSNVCAATWYSGA
                     GTLPCPGTDNDAKGFVLITNPSKLENGTTDNRSGLITFPQNINNGYIQGIYPSYKVKS
                     GDKFRAVVNCESGATSCYVVFRLDYSVSGSSSIQTFWAFVERYEGQYYTADIDLTPLA
                     GQDLKFILTVLSTGSATGDRALWVSPIIYNASSGSGSSSASPTPTFTVSPTLQATISA
                     TATTPSPTSTVTVTVPAATATSTPGTPTPPTPTLTLTPDVTATPIPPSATPTP"
     gene            12951..14114
                     /gene="dinB"
                     /locus_tag="PLE39_07770"
     CDS             12951..14114
                     /gene="dinB"
                     /locus_tag="PLE39_07770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNE68262.1"
                     /translation="MPRTILHLDLDAFYCSVEETQNPALRGKPFAVGGKPDERGVVAS
                     CSYAARALGIRSAMPMSRAVRLCGNLIIIPGRHKLYGEYSEKVMDKLRNLTPLLEQIS
                     IDEAFLDISDIRESPARIALDLQAGIKNELHLPSSIGIASNKLVAKIATEVGKKSSKK
                     KNEPPFGLTIVPAGEEAAFLAPLPADMLWGVGPKTSARLTELGIRTIGDIANWPEKDL
                     VHLFGENGRDLWRHAHGIDNRPIVTEYETKSISQETTFNVDVRDDKTLEKTLREQAKE
                     VARQLRKNDLAGKTVKIKIRWPDFTTLTRQVTLPTATDSEDDIIKAALKLLQAIRKPN
                     QAVRLLGVGVSGIGAPVRQLSLWDAGSEKSRKLQEVVDQLQEKYGRNVIHRGE"
     gene            14201..15166
                     /locus_tag="PLE39_07775"
     CDS             14201..15166
                     /locus_tag="PLE39_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HNE68263.1"
                     /translation="MSNVATRLITLILTLQSQPNQKASDLANKLGISVRTLHRYFSML
                     DEMGIPVYAERGPYGGFSLVRGYKLPPLIFSVEEAVAVYLGTNLISEAWGKLYKDSAQ
                     SAMAKIENILPNEQRDEIHWARRSLVTTSLYRADLASLSPIMEKLRSAARELRQVNIT
                     YQGQTNAKAEKRRIDPFALVFRAGWWYLVGYCHLRNAPRTFRVDRIQKLSVLEKSFEA
                     PADFNIHAYLENEFKNQPSIRAKLRFIPEAAHMVKANPSMWETVTENSDGSMDVTLTA
                     PDLPWLASMALSFAHWTRVLEPQELQVMIREWAQATADLYSEITR"
     gene            15175..15669
                     /locus_tag="PLE39_07780"
     CDS             15175..15669
                     /locus_tag="PLE39_07780"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HNE68264.1"
                     /translation="MSKPDSKDQMLKAAQEERDALEALLNTLTTEQMTQPNIVGEWAI
                     KDVLSHLMEWEKMVMSWYVNGLKGKPTFVPSREFNWGQLPQLNHAIYLKHRDKNLADV
                     QKEFKASYKKIIKTIEGIPEKELFTRKIYAWTKNNLLAAYFISCTSSHYRWARTVIRK
                     ATKS"
     gene            15684..16328
                     /locus_tag="PLE39_07785"
     CDS             15684..16328
                     /locus_tag="PLE39_07785"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNE68265.1"
                     /translation="MKTLDLKKQFKYLYQPSAKKIEAVQVPNLQFAMIDGAIEKGSEP
                     GKSPTFAEATQALYSLSYTLKFMLKKRKTNAVDYPVMALEGLWGVEDGFFDIAIKDNW
                     TYTLMIMQPDIITSEDFEEAREQVRKKKGDSEMLSKIRLAHFEEGLCVQTMHIGPYAT
                     EPATIDRMKEYMAANKLKDNVGPNGMHHEIYLSDPRKASPDKMKTVLRHPVIAL"
     gene            16374..18761
                     /locus_tag="PLE39_07790"
     CDS             16374..18761
                     /locus_tag="PLE39_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68266.1"
                     /translation="MKNRFALFILIAVLIGSCAPTPSNTATASLPSSTVIADSPTATI
                     APSATPTPVPITLPVDRFNPIPELNPITTENIDALEEVAFFDNSPISTHITEDKSRLT
                     VWFESGLQTFDMPTLTPYPFIRMNMSIFSSNDAISEDGRLFARAYPGVKAEFWTPTIF
                     IWDTETGEEVCKIKLSEHRGNFFYTNFHLEQNTFSIHGDLGPNGKHKVIIYGLNTCQR
                     IFDRDSDSAMSAISPDGKYAAVTQKGQVVIYDTSTKSHNAIGETRGAQGVAFLPDSGE
                     VAISYSGQTFLYDMTSGEVNGFFDAGISNFYSTISVLDHGEWVVISASEKNYFWNSSE
                     KQVYSVQEFIGRDFKFSNGILTTYKTVFNLRTKAKISIENYGPQAFTALSSDQSYLAV
                     SSNIPPFVTDIYETATGKQINSIKGVYGPITVGSDSFIASSHEVGKVFNFTTGETDQD
                     LDRSYLSGFLLGEKEIVTWDALGKISLLDIKTGKAVTAANLPVFPSSFLNYTRYKNAT
                     PLWDELSQSDFASLPPSNTMVSNDRTIGIQENNGQLQIFALSDGNFIPATDDPIAAYN
                     LTNLGFQFKFSPDDTIIAGANYNSLTLWDGQTGDQLERIGLPNGAVTDIRFSADGTKI
                     VISKNDGPPQINAFGNLSLIIFDVLEKKIVQDYKLNQQLNKTGCNTTLPFALTSDGGR
                     VATLTQDCKIGVYDSLTFEELSSFGSPYSDSNPAFSISPDNNLLVVVDRKNIEIWDIK
                     TEKRIRKMDIPELAGTFDFYAHKAAFTSDGKMIIVNSTFWSQSSVTTFWAVPSIQ"
     gene            18802..19161
                     /locus_tag="PLE39_07795"
     CDS             18802..19161
                     /locus_tag="PLE39_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68267.1"
                     /translation="MNCFYHPDQPAVGLCKYCQRGLCLNCATVVDDVLACKDRHEDEV
                     HQLEQLTARNLFQSKRVASGYMRNAIFYGLVGAAFTGFGLWQLKWLGLQAVVFIMLGV
                     FLFYAAGANYLEARKHK"
     gene            19163..19909
                     /locus_tag="PLE39_07800"
     CDS             19163..19909
                     /locus_tag="PLE39_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNE68268.1"
                     /translation="MGTWICHLRIAEKLLTHFPELDKTLFTFGNLAPDSGIPNETWTE
                     FDPPKEVTHFLQKGEGEDLIRDLVYYREYLANLHLYDDPQKYSFRLGYFTHLICDILW
                     MKYIGSTTKIAFHELFEQDKQKAVDLVKNDWYGLDQLYNRAHPENLFWQVIYKTPNPP
                     SYLPFVQEKALHQQYAHIRKFYGEPDSEWFIERPYPYLSESSMTRVVEDTVQATLIIL
                     EKIKYTDINKTQSSTEILPQEFMRPYSTPL"
     gene            19943..21199
                     /locus_tag="PLE39_07805"
     CDS             19943..21199
                     /locus_tag="PLE39_07805"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE68269.1"
                     /translation="MNEFENFFNLSTDPMTIADANGKIIHANSVYTAITGWSVDELKA
                     TSYWDLLHPDDHPKARAALQTVKAGHPVFALEYRFLCKDGTYKTFIANLSGNKKSEHL
                     YAISRLKEGQIVPYIMVANAAPVAVIIINTSGTIIHANDLATTLFGYEAGELQEKSIE
                     DLIPMRLRKKHQAHRGDYQSNPVNRPMGSHYNLKGLQKNGLEIRVDIALNPLYEQNEL
                     YVACSILDITEKITTAELALNLERENLRLARLAQRDPLTQAYNRRTMEEMFPKIAEEC
                     KKQNRSVSAIMLDLDNFKTFNDSYGHQTGDQLLKELAYIAQNHIRENDILVRYGGEEF
                     FIIMPNCTPHQASEVAERIRSSLEKDMTSGHKITASLGISTYGFSNGPGEPLDEILEK
                     LIEQSDQALYQAKDKGKNRVQHFSSI"
     gene            21257..21448
                     /locus_tag="PLE39_07810"
     CDS             21257..21448
                     /locus_tag="PLE39_07810"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNE68270.1"
                     /translation="MNEVKTLTRSKSNRMIAGVCAGLADYLNMDPTVVRLLFVIGFFT
                     LNGGMLVAYLIMAIVTPEQ"
     gene            complement(21483..22388)
                     /locus_tag="PLE39_07815"
     CDS             complement(21483..22388)
                     /locus_tag="PLE39_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68271.1"
                     /translation="MTEAVITVQNLTRRFGDFIAVDHINFEVQAGEVVGYLGPNGSGK
                     TTTIRMLLGLLKPSDGKATVLGYDVFKESEEIRMRVGYMSQKFAIYDDLTTLENLTFY
                     GGVYGITDKTRIQRTLELVGLNGHEHTLTRDLSTGWRQRLSLGIALVHEPKLLFLDEP
                     TSGVDPTARRAFWDLIYELAESGVTILVTTHYMDEAEYCERVGVMRDGKLLAMDTPTA
                     LKHSVITGDVWEVYAYPLEKGLEILSSVQGVERVGLAGDHLRVISSQVEKEAMQSLLS
                     AKSLRVEKIERGEATLEDVFLSLAR"
     gene            complement(22381..23352)
                     /locus_tag="PLE39_07820"
     CDS             complement(22381..23352)
                     /locus_tag="PLE39_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68272.1"
                     /translation="MNDYLVQANGLVRHFGSVHAVDGVDLKIKPGEIYGLVGSDGAGK
                     TTTMRLLVGALLPSQGEITICGYDVLKQAEQARSTIGYLSQRFSMYEDLTVLENIRFF
                     AEIRGLSTTEWLPRSMEILEFVGLEKFKDRRAGQLSGGMKQKLGLASALVTRPRLLLL
                     DEPTTGVDPVTRQDFWQLVIKLVSQPVSSQPETLQSEGVSVIISTPYMDEASRCHRVG
                     FMKKGKIIAEDTPSSLRSRLNGRILELRGNPINTLRHVAHQDSGVEDVAAFGDKLHVR
                     VLEGQADLIMKRLPEEIKAAGGTLTDLRIIPPALEDVFIALSEVSND"
     gene            complement(23412..24716)
                     /locus_tag="PLE39_07825"
     CDS             complement(23412..24716)
                     /locus_tag="PLE39_07825"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE68273.1"
                     /translation="MNHKSIPVPVRIVLALVVLGAIGYLAFLTLNTNGNGELTASGSI
                     EAVIVNVSPELAGKVTGVDVAEGALVNANDPLLHLDPSLLTAQRAVAASALDSAKAAL
                     TTAQTKFDQTLQAALSAQDAQRAKDLRVSAPDEFNQPLWFIDQTDQLAAAQTEVDAAK
                     IALDEANANLEKVISDLGNAQYLDAEKKLSEARAAFLIADKVKVQAENAVEAGAVQDA
                     AYDYYNDVLDELQRAQDDFNALTNTEAEDDIKYARGQVVIAQQRYDAAYTRLLSLQTG
                     TESPAVISAKNTLDQAQTAVDQAQANLDLLDAQIAKLDVYAVMSGVILSRNVEPGEFV
                     QPGAVALTMADLTNLTITVYVPEDRYGQVSLGQTAEVSVDSFPGLTFTATVVHIADQA
                     EFTPRNVQTVEGRSSTFYAIKLQVSDPDGKLKIGMPADVVFK"
     gene            complement(24767..25894)
                     /locus_tag="PLE39_07830"
     CDS             complement(24767..25894)
                     /locus_tag="PLE39_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE68274.1"
                     /translation="MLNSRISSIIRKEFLQILRDPRTLAMIIIMPIMELFLLGYAATT
                     DVKNISMAVWDQSKTTQSRALLDAFRAADYFILEYSVGSTDEYQALIESGDTRVVLVI
                     PADYDRRLIEGNAQVLMVLDGSDASVGATALSTARLVGQAYSTKILSEQTLQSGRGAP
                     SAPLEVRTQVWYNPDLDGAYFNIPGVIGMILSFITSILTATTIVRERERGTIEQLIVT
                     PIRSWELIAGKLLPYVILGFVETFEVLLIGHWWFGVPIRGSAVLIIVTSGLFLMSSLG
                     IGLFASTMANTQQEAMMTVMMFNLPMIFLSGFFFPIAAMPRFLQIVSYAIPLRYYLII
                     IRSLLIKGVGLVAIQNEVIALVIFGSVIMGAAALRFRKRLD"
     gene            complement(25904..26521)
                     /locus_tag="PLE39_07835"
     CDS             complement(25904..26521)
                     /locus_tag="PLE39_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE68275.1"
                     /translation="MSAKSKDAPKKGEVTRLAIEDAAIELFMEHGYHATSMRQIADQA
                     GLALGGIYNHFASKEEIFEGIIIDKHPYKKILPLILEAKGDTAEEFLRNAMRIAITEL
                     SKEPYYIKLMMIEFVEFNGGHGAAMLKEVAPKVLPVFEQIVKIRKNLRVTNPAMLMRS
                     FFGLVVSYFITEMVVSNSIVGSLMPKNPMDVYVDVYLHGILKPES"
     gene            26622..27905
                     /locus_tag="PLE39_07840"
     CDS             26622..27905
                     /locus_tag="PLE39_07840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE68276.1"
                     /translation="MLPFTTLEKQIRADLKYNIKVNLWDGALFGVALGFASFGTVLPL
                     FVASMTNSALLIGLVPAIHSVGWQLPQLFTASYVSRLRRYKRSVMMMTIHERVPFLGF
                     GIIALLLPMIGLKVGLVITFLLLIWQGLGGGFTANSWTSMISKIIPPENRGTFFGFQA
                     GLANLFISGSAVAAGYLLDYLDSPLDFAACFFIASIFFTLSWFALALTREPEDTEKII
                     PEEKTHFWDDSKKILKKDFNFNWFLVARFISQFATMGFSFYIIYALRRFNMDAITAGY
                     LTATLTIAQTIANASMGWLGDKWGHRSMLIIGAAAALASATLAWLATSIFWFYPIFLL
                     TGFANVSIWTIGMAMTVDFGTEAERPLYIGLSQTLTAPATILAPLLGGWIVDAAGFIP
                     TFSISIILSIAMIGILVFLVKDPRMHHSRKMKEVK"
     gene            27952..29142
                     /locus_tag="PLE39_07845"
     CDS             27952..29142
                     /locus_tag="PLE39_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNE68277.1"
                     /translation="MSLIKEKVNQAIEILKEQQIDMWLTFVRETSGVRDPALDFLIGE
                     NDLTWPSALILTRKGEKIAIIGNLEKESLQRLDVFDEILGYDTAVSTLFKETITRLNP
                     DRIAVNTSRNNVHADGLTHAMYEFLREYLAGTPYADRLVSAEPVISAMRGRKTATEQA
                     RVKKAIEITEEIFAKTFDFIKVGMTEIEVGAYMHQLAKDYNVDLAWPAENCPAVNSGP
                     NSPVGHNGPTDIKIERGHLVHFDFGVKYEGYCSDIQRIAYVLREGETEAPIEIQRGFI
                     TIRTAIEKSREAMKPGVTGNVIDTISREIVADAGYPDYKYALGHQLGRVAHDGGALLG
                     PLWEKYGDSPNQKLEIGQIFTIEPGLPVNGYGYLGLEEDVVMTKSGAEYFHEPQKEIV
                     LLKG"
     gene            29329..30882
                     /locus_tag="PLE39_07850"
     CDS             29329..30882
                     /locus_tag="PLE39_07850"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNE68278.1"
                     /translation="MTQEDPRILELRKMRAKALEGGGEERIAKQKAKGKMTARERVIA
                     LLDPGTFNEIEPFITHQGDELDLLKDKFYGDGVITGYGQINGRTVYLYSQDFTIYGGT
                     LSEMQSHKICRVMDLAVRNGVPFISLIDSGGARIQEGVRSMGGYAEIFRRNAQYSGVV
                     PQISVMLGPCAGGAAYSPALTDLVIMVEKQSFMFLTGPEVIKAVTGEMIDAESLGGSG
                     VHTSVSGVAHLSVQSEKAALEMARHFLSYLPSNNVENPPFTPTTDDLARMDEELNHMI
                     PLEATEPYVMHQVIEKIIDQGSFLEIQPDWARNAIVGLARIGGHSVGIVGQEPAIMAG
                     VIDIDAADKMTRFVRMCDCFNIPLVTFVDSPGFLPGIAQEHGGIIRHGAKLLYAYSEA
                     TVPKICIITRKAYGGAYVVMSSKYLGTDVTYAWPSAEIAVLGAEGAANILFKKQIETA
                     NDPTAERKKLANEYREKFNNPYYAASTGYVDDIIEPRESRPKLIATLSALRDKYAPAP
                     PRKHGNIPV"
     gene            30937..31302
                     /locus_tag="PLE39_07855"
     CDS             30937..31302
                     /locus_tag="PLE39_07855"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HNE68279.1"
                     /translation="MTTDLLLSLQITALGMGLVFGAILLLWLMMALLTRFTAEKESAA
                     ETDSTAQTGASDSPEPAPVSTSGFKAQAAALAVALALAEQELSSAHPLLEPPTAIVSA
                     WQLGMRTSQRSEKGNFRRH"
     gene            31331..31792
                     /locus_tag="PLE39_07860"
     CDS             31331..31792
                     /locus_tag="PLE39_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNE68280.1"
                     /translation="MKYKVTVNNQVYEVEIENINTRPVIAHVDGERFEIMPENAGQAE
                     TGKDSSIPTEKKAFNPSPVTAAKPSPNPALSGNILTAPLPGTIVEVFVKPGEKVEAGQ
                     VVVIIEAMKMKNSIRSVYSGTVNEVFVSAGQSVAHKQALVKFADLGEASWM"
     gene            31833..>32941
                     /locus_tag="PLE39_07865"
     CDS             31833..>32941
                     /locus_tag="PLE39_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNE68281.1"
                     /translation="MDLTSLLPELLKGFTHFTLGNGVMIFAALILVYLAVFKEIEPVL
                     LLPIGFGCLLANIPLAGMTAAEGMSKVLYDAGIKTELFPLLIFIGVGAMIDFSPLLAQ
                     PRMVLLGAAGQFGIFGTLMLAIALGFPLNQAASIGVIGAIDGPTSIFVATKLAPELLA
                     PIAVAAYSYMSLIPIIQPPLMKLLTTKKERMIQMDYAPTPISKKTLIFFPVVLTIVVG
                     LLVPEATPLISMLMLGNLLKVSGVVDRLSNAAQNEIINIATLFLGLAIGSTMSAASFL
                     NLDTGKIMILGLFAFALDTVAGLLFGKLMAVVTGGKINPLIGAAGISAFPMAGRLAAK
                     TANDEDPNNFILMHAMGANTAGQLGSVIAGGILLSV"
BASE COUNT         8795 a         8478 c         7986 g         7682 t
ORIGIN      
        1 ctactgcgga gaccgtgaac gcgccaaagg tttttcaagc tgcaaatcaa aaagaataag
       61 cgcaagaaaa gttaatgcgc ttgtcctcga tgcgatccta aaccgcatat taaccccgca
      121 ctactttaac gccctgctcg atgagacgaa aaaattatac gtggacacgg agaccattga
      181 cgcgcagctc tcacaaaagc ggctcgatct ttcctacacg gaacgcgcaa taaaaaatct
      241 tttagaactt gtggaggcct tcggcgtgaa tgaatccgca aaagaaaggt taaggcaaaa
      301 agaacttgaa aagctgaact tgatgcaaga gatcaaagac atagaaaccc gtaaaaggtt
      361 aatcaagatc gaagtcaccc cggaggctct cgccctggtc tttgagcaat ggcgcaaaca
      421 gatcattgat gcagataaaa ataatgacgt ggcagccgtc cgcgccttgc tggctggctt
      481 tattgatcgg gttgaaattg aaagcccgaa tgttacaatc cgctacaaat acccaataca
      541 atcgttaatt gctacctcac ccgatcttct ccccgtgggg gcaccatagt gaacaaaaaa
      601 cgctggataa cccagcgttt tttgtttttt tgcactttaa atggctgatt ttagtttttt
      661 ttccttgcgg ggaaaaaagg gataaataaa agaaatccca atgcaatcac gccctggccg
      721 atcaaaacgc ccgtggcctg ccacggccca aaataaggca ctttcggaaa ggattgtgaa
      781 cccatgccaa agacatcagc cagacccgca aaaacagcaa tgacatagcc agtaccaaca
      841 agccttgtcc caatatcggc gaggatggtg cgttcctgcc catgccacaa ggttagcaga
      901 ctgatgtatc cacccaggca gatcaaagca agacccacta aaaagacggc gatttgcaca
      961 aagccaacga ccgcactacg atcccaaccg aaccaatcag gtttggaacc gatcgtgaaa
     1021 ataaacaaac caacaaaggt aagcgtcaac ccgacataca ctcgcggcgt attctggttt
     1081 tgagcggaat atggcgctgt atctacgggg gtggatgctg aaacaggttt attcatactc
     1141 atgatggcaa atcccttctt aggcggttga gccagttacc gccaaggata ttggagacat
     1201 cgacatcgtt ataaccacgt gccagcaaca aagccgaaag tttttgcaga tcagcaactg
     1261 tctcaatttc agggggaaca gattgcaacc caaagccgcc gtcaaaatcg gaaccaatac
     1321 ctgcgtgaag ggagtcacct gcgatctggc aaatatggtc aatatgattg gcaacatggt
     1381 caagtgaaat ttcactccgc tcgcttttgc cagccaccca ccccactttg aggtatccat
     1441 tgaatggcac cactccaata acaccgtccc gttcaatgat cccttcaatg atgcggtctg
     1501 aaaaatgacg gttcgaagga gagttcggca tcaatgacag gcagttggca tgcgtgccta
     1561 caatcggtcc acgataaata tccaaggctt ccactgcagc ttgttcatcc atgtgactga
     1621 ggtcgaggat aaagttatag tctgccatgg cggcgagcag tttacggcca tctggagtca
     1681 atgggccagg ttcattccaa ccaccacaat aacgggtgcc aacccaggca gggccgatga
     1741 ggcgtacgcc catctcgtgc cactcttcca attcagaaag ggtacggatt ccttctgcgc
     1801 cttccatgag cacaaccatg cccacggggt gtccgctgct ggctgtggcg gggaggctct
     1861 gccagttttc cagaatcagg tctaagtcac ggtttgatgc gatgagtcgg aacttgtcgg
     1921 gcattgcatc ggtcatccga tggtaggtca gcagttgttc gcgataaagc tgatgggctt
     1981 catcgtaagt tttgtatacc tgggctttgt tttcgctttt gctccagcgg ataggggtgg
     2041 cgtagagggt tgaaaagatg accgcaacct ggccattttg gtattcgggc caaccaagca
     2101 gagtatcatc attttgcttt acaacatcac tgcctgcttc aatctggcgc gtttccacgg
     2161 ctgaacgagt gtagtcacgt ccatatttga gcatgttcca ggcaatatct gcatgtgcat
     2221 caactatgat aggcatggga gattagtgaa gagcgcacct ttcggtgcgc tctggattat
     2281 ttttctactc tttgttctct tcgtcggggt cctggaaccc ttgtgtaagc atcttgaagt
     2341 ctttgtcggc gaaggcggcc gagtcaacct tgcggaggct aagaccaatg cggtgctgtt
     2401 caccgtcaat gcggatgacg cgcaacgtct tgacttcacc ttccttaaga acttccttgg
     2461 gatgctcaac acgattctcg ctcaactcag agatgtggat gagaccttca atatcgcctt
     2521 caagacgggc gaatgcacca aacttggtca ggcgggtaat gacaccttca acaagctggc
     2581 cgacgctgaa tttctcaaca cgactcttcc aggggtcatc ctgtaaggcg cggatggaca
     2641 gaccaatgcg tttcttgtcg cggtcaatgt tgataacctt gaccttgact tcctgcccga
     2701 cttcgagaat ctccttggga tgctggaggc gatcccatga aagctctgag agatgtacca
     2761 agccatcggc gccgctgaca ttgacgaatg cgccaaaatc tgcgaggctt gtgactttgc
     2821 cggtgtatac cttgccttct tccaactcac taatgacacg gtccttcatc gaggagcggg
     2881 tttcagtgtt ggtggagcgt tcggaaagaa taaggcggcg gcgttcgcga tccacttcaa
     2941 taatgcggac ggaaattgcc tgaccaacca tcttctgcca acgctgttca ggggtatcac
     3001 ctgaagactg cgcacggcgt acggcgctga tctgtgaaga aggtacaaaa ccacgtagat
     3061 ttccaacagc agcgatcaaa ccgcctttgt tgaaaccaac gatcttggta tcgatcacgg
     3121 tttcatctgc gatcatcttc tcaacatttt cccaagacat ctcttcctgt gcgcgcttga
     3181 acgataaaac gacattgccg ttttgatctt caggattgag aacgtaaacg ttcacttcct
     3241 gtccaacctt gagcgcggcg cgttcttcag ctgtgagttg ttccaactca cggccagcta
     3301 caacgccttc ggattttgca ccgacactga tgagaatttg gcttggtcca atgcttgcga
     3361 tcatgccttt gcgaatttcg ccgacctgcg gcaactcgac actcaaagat tcagattcga
     3421 gcagtgactc cattgtctga ttgtggggtt ggtctccctg gggatttaca gccccttgcc
     3481 cagtttgggc ttcattttga tccatcacgc gtgactccaa ttaaaactga aatatgattt
     3541 cgattataca agtgattaca gaacttgaca accccgatat acgcattaaa acaacagatt
     3601 cttacccgaa agaaaaaact cgcttataat cacgctctgg agaaaaaatg cccgctatct
     3661 tcccatttac agccattgtt ggacaggagc gcatgaaacg cgccctcatt ctcaatgccg
     3721 ttgatacccg tatcggcggc gtattgattc gcggtgaacg tggtacggca aaatcaaccg
     3781 catcgcgttc gcttgccgct ttgttgccgc atgtcaagat cgtacaggat tgccgcttcg
     3841 gttgcgaccc ggataaatca tcgacatggt gtaccgaatg taaagaacgt tatgccgcca
     3901 gcaaaaaact ccccatcgca gaacgcgcca ctccatttat caatctgcct gtttctgcca
     3961 cggaagaccg tgtggtcgga acgctcgata tcgaaaaagc gattcaaaaa ggtgaacgcc
     4021 attttgaacc tggtgtactt gcaggtgcca atcgtggact tctctacatt gatgaagtca
     4081 acctgcttga tgaccatgta gtggatattt tgctcgactc cgctgccatg ggtgtgaaca
     4141 ccgttgaacg cgaaggtatt tcttttgcgc accctgcccg tttcattctc gttgggacga
     4201 tgaatcctga agaaggagat ctccgccccc agttgctgga tcgtttcgcc ttatccgtgg
     4261 acattgtcgg tattcgtgat gctcgtgaac gcgtgaccat tatggagcgc aatctttcct
     4321 acgaaaagga ttctgaagcc ttccgcaaac aatggcttcc caaagaagaa gaactttcca
     4381 gcaagattgc ccgtgcccgc gaactcgtag accaggtcac ccacaccagc cgtgacttgc
     4441 tctccatcgc tgccttgacc gcctccttga acgtggatgg tcatcgtgcc gaccttgtca
     4501 ttctcaaagc cgcccgcgct gaagccgcct tcgaaggccg caccaagatc aatgaccacg
     4561 atattgctct tgccgcagaa ctggcgcttc cccaccgcat caaacgcacg cccttccaac
     4621 aagccgaatt gaccaccgaa cagttgcagg aacgcatcga acaactggcg gggcaatcca
     4681 tgcctgaaga ttcggatgaa aaatccgaaa gcaaacagga tggcgaaggc gaggaaaaaa
     4741 aaacttaaag cttgaagatg agccacagga aggtccccag cagggcaacc ctgaacccat
     4801 gcagcagcag gaagtttttg caagttccaa tgcgaattgg tgggaaggcg gacaaaaagt
     4861 aaaagctggt gagaccttcc agcctcgtaa acttaatacc ccactcgata aactaacccg
     4921 caagcaagct ggccgccgtt cgctcacaaa aacggagcga aaacatgggc gctatatcaa
     4981 ggcgcgaccc gcaggcgaca aactcaacga catcgccttc gatgccacct tccgtgctgc
     5041 tgcaccattc caaaagcaac gcaccgaaga acgcaagaag ctcgcattct ccatcaagaa
     5101 aagcgacctt caacgcaaga tccgtgtgaa gcgggttgca aatttggttc ttttcctagt
     5161 ggatgcatcc tggtcaatgg cagttgcgga acgcatgaat gcaaccaaag gcgccatcct
     5221 ctcgttattg accgatgcct atcaacgccg cgaccgtgta ggcctgatcg tctttcaaaa
     5281 ggatcgcgcc accctggtcc tctcccctac gaactccgtt caactcgcac aacgcgccct
     5341 gatggatatc cccgtcggcg ggaaaactcc actctcggct gggttgttca tggcgcatga
     5401 tgtgctcacc cacgaaaagc acacccaccc cgatgtggaa ccgctcctca tcgtgctgac
     5461 cgatggtgct ggcaacgtat cccttggcac cttgccaccg caggaggagt ctcataaatt
     5521 tgccgaactc atcgccaacg aaaagatacg ctccgtcgtc atcaacatgg aacacgccgc
     5581 cttcgatcag ggacttgcgc aagaactcgc aaacaacctc aaagcgccct gctatgcgct
     5641 cagtgaattg aaagctgaaa gcctgtacaa cactgtcatt gatgaaatgt cgaagccagc
     5701 gaagaagtaa tcaacacaca acagacctct tgtaaaatct gaaagtttaa ccactgagcc
     5761 acggagacac agagaaccaa attaaaaact ccgtggctca gtgtctttgt ggttcaaaat
     5821 ggtatttcaa agacattcgt tacagcatac gcaggatatc aaataaataa gacacagaaa
     5881 catagaggaa atcaataaac tccatgtttt tgtgtctttg tgatttaaaa gtttttagtt
     5941 gcctacgtgt tcaaaaaaac aactaaacat ccaaactgcg gttacgaatc aaaccccagc
     6001 ccgagtgcat ccaagatctt caaccataga tttctttgtc cgccattatc gtcagcgcga
     6061 ttcaaagacc atcgtgtgaa gttgacgatc ggggaacgaa atggctcagg cgggaacgga
     6121 aacggtttcg atttcaccat ttgcaattcg gtgcgttcgg tctttttgcc atccaacaga
     6181 tccaacatca cttgcgcgcc aaagcgtgat gcgccaacac ccagacccgt gtagcccatg
     6241 gcataggcca acttcccacc ataagcctga ccccaaaacg cagtgtagcg tgaacaagta
     6301 tcgatcactc caccccaggc gtgtgtgaaa cgcagaccgg tcaactgcgg gaaggtttgg
     6361 aaaaaatgct ccgccaaacg agcaaaagtt tcggggcggt tttccaagtg cggagctact
     6421 ccgttgttct ggtaataaat ggcatcgtat ccaccccaca aaatcctgcc atcggcagtc
     6481 gtgcggtagt aatggaattg attcccatta tcgctcacac cttcgcgtcc atgccagccg
     6541 atcgcatcgc gctgttcctt tgagagcggt tcggtcatca gcacataatc gtaaacagga
     6601 accacataaa aagaaagccg tttcagcaaa ggcggaaacg cattcgtggc aagcgccaca
     6661 cgtttggatt cgacctgtcc gtatgatgtt ttgacgagaa tcgaatcgcg cttctcttcc
     6721 agactcgtaa cctgtgtgcc ctcaaacaag cggacaccaa cctccaggca ggctcgcctc
     6781 aaaccccaaa ccaatcgtgc agggttgacc attgccacag acgaatcatg gattgcagca
     6841 agataagtcg gtgaattaac ccgggcgcgg atctgctcac gatcgaggaa ttgaacatgc
     6901 tctccatact ctgcagaatg ttcggcttcc tcgcgcaact cttcgatctg gtattcttca
     6961 ttggcaatat tgatctcgcc tgaacgtatg aaatcacaat caatattaaa ttgcttgatc
     7021 gtggcttcga ttccatccag atttttatgc cccagctcaa tgatcttttt tagctctttg
     7081 ggccagcggt tcaacccatt ttgaaaggaa tgggtcagcg atgaggcgac gaaaccgcca
     7141 tttcgtccgc tggcgccgat gcctgtttcc ccagcttcga gaaggaccac ttcacgagat
     7201 gggtcggctt gctttgctag aagggcggtc cacaagcctg tgaagcctgc accgatgaca
     7261 acaagatcgg ctgtgatgct ttcggtcaag gccgaagcag gctccggacg tgccgggtca
     7321 tccaaccaga acggcacgtg tttcacatcc gcaagagatt tcaaggtctc agggcgcgga
     7381 gtgttcgaga aaatcattag gtctccattt tcgcggcatg gccgcctcgc agtgagtatg
     7441 caggccatgc tcatagattt gttgtagttt acgctgtaga tgtagaggag tcaaatatca
     7501 aattatttca caagggtttc ttgcgggtca tcgcggtaaa caaaccgcct aggaaaattg
     7561 cggccacgcc aatggcggcg atccccatca tgttttcccg tctttgttcg ggggtcaggg
     7621 ttggggttgg ttctggctct gtagttttgg caggcaccat ggtcggggtg gggaatgttt
     7681 ggggtgactg ggcaagtgtc ggtgcggtgg atgcgccgcc gggggcgggt tgcgtcgccc
     7741 ctttcagcac cagcagtttt tgtcctgtgc gcaaattcgt gtcggtcaat ccatttaact
     7801 gctgaatttg tttaatggtg gtgccgtagg aaatcgcaat gctccacaag gtttggccgt
     7861 atttgacttc gtgatagaca ttgccatcag gcatggcagt gttgagaaag acgggagcaa
     7921 cattttgcgg aagcaatcca ttttcagccg caagggttgc catgcttgaa taggcggcct
     7981 gggtcatggg gatgcctgtg aggtaaacaa tggcatcgta ttgcatctgt ccgcttttgg
     8041 tttgaagcgc agtctcaagt acaatgacat acacatcttc atcgggcatt tttgcgatgc
     8101 ccacgccaat atcgctgcgc tcaagagaca gcattgtgtt ttcgtgttcg tcatcgctca
     8161 gccacatggc tacggcttcg tcggcattgt gggcgaatcc ccagttttca gaacggtagc
     8221 catcgagtga aatatcaccc gaaagcggat atccgagcga aagcagccat tgtccaagcg
     8281 taatgccttc gggccgccaa tgaccttccc tgcctgccgc aatgccatct gcttccatct
     8341 gaccgatttg catcaaaaca gggtgtgcat tcaacggtgc gatgccgtgt gaaaggcgca
     8401 ggttgttgac tccttccatg atctcagcag gcacaggcgg gcggtcagaa gagggagtcg
     8461 gtgtttcctc gctttgagct gagggatgtg cgaatgcatc tgaaaccgaa aggaagccaa
     8521 gtgatagaac aaaggctgca aagactgtga tcaaaaaggt ttgatttttc atatgcgcga
     8581 gtttacagaa ctcatggtct ccatgaaggg gcgtaccagt caaaatcggt ggaaaggccc
     8641 ggggcttgca gtattttctg ctccagtgtg gcaaggtcaa tgaggaacaa atgcccgcct
     8701 gaaatggaag tggtctcctc gaccaacagg gttctgccat ttggagaaaa aagcaaacga
     8761 ccaatggtgg tgcccgtata aaccagcgac tgggtctttc catctctgct gatcacatgc
     8821 acttcggcaa tcggcgaacc atcggcggaa ttgctgtaat gattgaaggc gatcagcgaa
     8881 ctatcaggcg accacaccaa cgggtagatc gatccgcctg caaaactggt gagggcaacg
     8941 gcattggcac catcaggctc catcaccttg atgacatggc cttgagtatt gtaatcaatt
     9001 tcgtcgtagg ccagtaaact ggcatcgggt gaagcaacga attgcgattt ggactgctgg
     9061 gtttcaaaca tcggtttggc ggctccgtca ctggcgcgca cgagaaaaat attccctgca
     9121 ttggagggca atgccgagcg catcaaaata tgatcggtgt accagccatc cataatgtaa
     9181 ggccgtcctt caacaggcag gttcagcgat acacttttca gttctgagcc atcacggcgc
     9241 acgacataca catcttcacc gcctaatcca tccagcgtgc ggaatgcgat ccaggtgcca
     9301 tcaggcgacc agaacggcgg gtcaatgtat gcgaactgcg cgatcgaagt ccacgtgttg
     9361 gcggcggggt caaagatcca tagtttcgtt ggagtaccat tggacgaatc ggaatacgaa
     9421 aaggcggcaa gttttccatc gggcgaccag gacaaagcgt tgatcgtaaa attaaaagca
     9481 aaaggaactg tcacaatttg catcggcgga caattggccg aatcaaatac acagatgcct
     9541 gaaacgcgtg ccaattgaat ggattgtcct gcctgccgcg gttgaaggaa gaaatacaat
     9601 tcacccgcat cttcgctgga cggggaacct gatgctgtta caacaggcac agcctccgct
     9661 tcagcatttg gcaaaccagc ctgttgcaaa ccagcaggag gcgtgaacaa agtttcatca
     9721 aaggtcacat caaaagagat gtgatccaca acccgctccc ctgccaactg actcgaattg
     9781 ccttttgtga attcctgtaa ttttagaagg atgcccgttt gggtatccac ccacattttc
     9841 agagagggaa gtgaattggc atcataggtc cattccacga cgagggcttc gcgacctgcg
     9901 atactttccg ctgcaagggg tttgaaaatc ccgccgtttt gtgaaaaatc ggaagcaaat
     9961 gccaattgcg aaagcggagt acccaactgc gtccagatcg ggtttgtatt cacctgcccg
    10021 ggaaccaacg gaggaatata ttgtccgacc cgaatgaaat ctggcacagg agctatctgc
    10081 acctgcgcaa gcgagaggtt tgcatcgtag agatttacac catcaattaa tttcaaccca
    10141 tgttgggcgg ggtcggtaat tccattcacc tctactttga atctgctttt gagcggctcc
    10201 aaccagactt gttcgcggaa ggtttgtgtt gttccatcag gaagataggt ggtaatggtt
    10261 ccatccattt gcagggtggt ccattgtgcg gcactttcca aaagcgcccg ctgaatttcc
    10321 tcggaagccg aatcaagttt caggtcggcg ggagcggggg ttacttcaaa aaaagaaggc
    10381 tcggcagggg cggaagtcgg tacagcaccc gagcaggcag ccagcacaaa gatcaacata
    10441 cccaacaaaa atcgtttcat gtattctcca aaacaaaggt gtgaccagtt gacgactacg
    10501 gaaaacaaaa gttcccgccg ccttattgtg ggcgacggga acaattacgc aagtggatgt
    10561 ttttagggtg tcggtgttgc tgagggcggg atgggagtag ccgtcacatc cggtgtaagg
    10621 gtaagggttg gggtcggcgg agttggggtg cccggagtgg aagtagctgt ggcagctggg
    10681 acggtaacag tcacagtgga ggtcggtgaa ggagttgtgg ctgtggctga gatggtggcc
    10741 tgaagagtcg gtgaaactgt aaaggtgggt gttggagaag ctgaggaaga gcctgaaccc
    10801 gatgaagcat tgtagatgat cggggaaacc cacaacgcac ggtcaccagt ggccgaacca
    10861 gtggaaagga cggtaagaat gaacttgagg tcctgcccag ccagcggggt cagatcaata
    10921 tcagcggtgt aatactggcc ttcataccgt tccacaaaag cccaaaaggt ttggatggat
    10981 gaactgcccg atacggaata atcgagacgg aaaaccacgt agcaggaggt agctccgctt
    11041 tcgcaattga cgacagcgcg gaacttgtca cctgatttca ctttgtaaga ggggtaaata
    11101 ccctgaatat agccgttgtt gatattctgc gggaaggtga tcaaccccga gcggttatcg
    11161 gtggtgccat tttccagttt ggatggattg gtgatcaaca caaatccctt ggcatcatta
    11221 tctgtaccgg ggcagggcaa tgtaccagcg ccgctatacc aggtcgctgc acaaacattg
    11281 gatacaaagt catagctggt gccagaaatg ggcgtagctg gagttacagg cgtgcccgga
    11341 gtcaccggcg tagcaggtgt ggatggggtg acaggcgtgc cagccacctt gatctcaacc
    11401 cagaaggatt tcgtaccatc agaaccgatt ccaaacggaa cgccagtgct gctcttgagt
    11461 ttccaatagc cgcgataggt gccagcgttg gcaggggcag tcagattgat ggtgatatca
    11521 acagtttgtc caggcgcgac agtggtgggg ataagcgcag agtcgggtcc gcccatcttt
    11581 tcgccagaat caaacatcag gctgtagttg gtccaggtgc aggtacccac gttcttgata
    11641 cgccaggtct ttgcaaaggt ggtgcccggc tggaaggtcg taccatccgc aacggttaca
    11701 tctttgatga actgggcgcg gtcacaggca ttcggcccgg gagtgggagt ctttgaagcc
    11761 acggtcgtcg gcaatgtacc ggtgactgtg ggagtgttgg tcgcaacagt gccgccagcg
    11821 cgggcaatga cagggttgcc ccacaacgcg cggtcaccca cctgcgaacc ataggcagag
    11881 atcaccagga tgaatttcac atcctgccca gccaacgaac tcaggtcaag attggcattg
    11941 taggtcaggc cttcatactt ttcgcggaag gtccagaaag tcttaacggg gttcgagccg
    12001 atctggtaat cgaggcgata tgccacataa caagaggtag cgccatactc acaaccaatg
    12061 gttgcctgga agcggtcacc actctgcact ttgaaagcag gatagctcgc ctgaatatag
    12121 ccgttattaa cattgttcgg aacgaagagc agcccagcct gcgctgattc aattccattc
    12181 tcgaattttg gcttgtccag tttcagggca aagccattgg cactgccgtc ggtaccgggg
    12241 aaggtcaaag tacccgcacc gctcgaccag gtggcagaag cggcgttcga ggtaaagtca
    12301 tacccagtgc ccgaagacga agtcacattg atctcaaccc agaacgattt gttcgcggtc
    12361 gaaccaatgc caaacacacc accctgtgtg ctcttgaatt tatagtaacc aaagtaatga
    12421 cccgccgcac ttggcgcaac catatccaca gtgatatcca cggtctgtcc ggggttcaca
    12481 gtggtcggca gggcagatga agcaggcgcg cccatctttt caccgctatc gaagatcaaa
    12541 gaaacatcgc cggcattcca cgcacaagtg ccgatattct ttaatcgcca ggtcttctta
    12601 aaggctgttc ccggggcaaa atttgtccca tcaggaatgg tcacgtcagc gatgaactgc
    12661 gcccaataac aagctgcagc ctgcgctggc tgtgttgccg cagagggcac aactgaaaac
    12721 atgaacacga gcaaaagcaa cacagcagca aatttagatg tgaatttcat ttcttcctcc
    12781 atgtaaagca aaagctttaa taatgacggt aattttcttg aaaggttcca tttgactata
    12841 cgccatccct agtataactt tactcataag aatgttcaat atattaacaa agcaatttca
    12901 taaccatttt tcctaccgcc gcaaccaccc agtacactat aatctcaatc atgccacgca
    12961 caatcctcca tttggacctt gacgcattct actgttccgt ggaagaaacg cagaaccccg
    13021 cactacgcgg caagcccttt gctgtggggg gcaaacccga tgaacgaggc gtggtggcat
    13081 cgtgttcata tgcagcacgc gctctcggca tccgcagcgc catgcccatg tcacgtgcag
    13141 ttcgattgtg cggtaatctc atcatcatac cagggcggca taaattgtac ggagaatatt
    13201 ccgaaaaagt gatggacaaa ttgcggaacc tgactccgct tttggaacaa atttccattg
    13261 acgaagcctt tctcgatatt tcagatattc gcgaatcccc cgcccggatc gcgctggacc
    13321 tgcaagcggg tatcaaaaac gaactgcatt tacccagctc gatcggaatc gcgtcgaaca
    13381 aacttgtagc aaagatcgcc accgaggtcg gtaaaaaatc gagcaaaaaa aagaacgaac
    13441 cgccatttgg tttgaccatt gttcccgctg gagaagaagc cgcctttctc gctcccctgc
    13501 ccgcagacat gctttggggt gtcggcccca aaacatccgc acggctgacc gagcttggca
    13561 tccgcaccat tggcgatatt gccaattggc ctgaaaaaga ccttgtccat ctcttcggtg
    13621 aaaatggccg tgacctctgg cgtcatgcac atggtattga taatcgcccc atcgtcaccg
    13681 aatacgaaac caaatccatc agccaggaaa ccaccttcaa tgtggacgtg cgtgacgaca
    13741 aaacccttga aaagaccctg cgcgaacaag ccaaagaagt cgcccgccaa ctccgcaaaa
    13801 atgaccttgc tgggaaaacc gtcaaaatca aaatccgctg gcctgatttc accacactca
    13861 caagacaggt caccctgccc actgcaactg acagtgaaga tgacattatc aaagccgcgc
    13921 tgaaactatt gcaggcgatt cgcaaaccca atcaagccgt gcgcctgctc ggagtcggcg
    13981 tcagcggaat cggtgcccca gtacgccaac tcagtttgtg ggacgcagga agcgagaaat
    14041 cccgcaaatt gcaggaagtg gtcgatcagc tccaggaaaa atatggaagg aacgtcatcc
    14101 accgcggaga ataagcggaa aatccaaaac aatcagcaaa gcggatatat gtcataattt
    14161 gacatatatc cgctttatac tttttatgca ggaacaagcc atgtctaatg ttgccacccg
    14221 tttgatcacg ctcattctca ctttgcaaag ccagccaaac cagaaagcca gcgaccttgc
    14281 aaacaagctc ggtatttcgg tgcgcacctt gcaccgctac ttcagcatgc ttgatgaaat
    14341 gggcattcct gtctatgccg agcgtggacc ttatggcggc ttttcgctcg tccgcggata
    14401 taaacttccg ccgctgatct tttctgttga agaagctgtg gcagtttatt tgggaaccaa
    14461 cctgatcagc gaagcctggg ggaagttgta taaagattca gcacaaagtg caatggcaaa
    14521 gatcgaaaac atactgccga acgaacaacg ggatgagatc cactgggcgc ggcgttctct
    14581 tgtaacgacc agtttgtatc gcgccgacct cgccagcctc tcacccatca tggaaaaact
    14641 tcgttccgcc gcacgcgagc tgaggcaggt caatataact taccaggggc aaaccaatgc
    14701 aaaagccgaa aaaagaagaa ttgacccatt cgcattggtg tttcgtgcag gctggtggta
    14761 tttggttgga tattgtcacc tgcgcaatgc gccacgcact ttccgcgtgg atcgaatcca
    14821 gaaattgagc gttctggaaa aatcatttga ggcacctgcc gatttcaata tccatgcata
    14881 tttagaaaat gagttcaaga accagccaag tattcgcgca aaactgcgat ttattcctga
    14941 agcagctcat atggtgaaag cgaatccatc catgtgggaa acggttactg agaattcgga
    15001 tggcagtatg gatgtaaccc tgaccgcacc agatttgccg tggctggctt ccatggcgct
    15061 gagtttcgcc cattggacaa gagttctgga gcctcaagaa ctgcaggtaa tgattcgaga
    15121 atgggcgcag gcaaccgcag atttatacag cgaaatcacc cgataggagg aatcatgtca
    15181 aaaccagact ccaaagatca aatgctcaaa gccgcacaag aagagagaga tgcattggaa
    15241 gcacttctca atacgctcac cacagagcaa atgacccagc ccaacatcgt tggtgagtgg
    15301 gcgatcaaag atgttttgag tcatcttatg gaatgggaaa aaatggtcat gtcctggtat
    15361 gtgaatggct tgaaaggcaa acccacattt gtgccatcca gagaattcaa ctgggggcaa
    15421 cttccgcaac tcaaccatgc catctaccta aagcatcgcg ataagaatct tgccgatgtt
    15481 caaaaggaat tcaaagcctc ctacaagaaa atcatcaaaa ccattgaagg cattcctgaa
    15541 aaggagcttt tcacacgaaa aatctacgca tggacgaaaa acaatctact tgcagcatac
    15601 ttcatctctt gcaccagcag ccattaccgc tgggcaagga ccgtgatccg caaagcaacg
    15661 aaaagctaat aaaaggagaa accatgaaaa ctctcgattt gaagaaacaa ttcaaatatc
    15721 tgtatcaacc ttcggcaaaa aagatcgagg ctgtgcaggt gccgaatctg caatttgcaa
    15781 tgatcgatgg cgccattgaa aaaggctctg aacctggaaa atcgcccaca ttcgcagaag
    15841 cgactcaggc cctgtacagc ctgtcttaca cgctcaaatt catgctaaaa aagcgcaaga
    15901 caaacgctgt tgattacccc gtcatggcac tggaaggatt gtggggtgtg gaagatggat
    15961 ttttcgatat cgccataaag gataactgga cctacacact gatgatcatg cagcctgaca
    16021 tcatcacatc cgaggatttc gaggaagctc gtgaacaggt ccgcaaaaag aaaggcgaca
    16081 gtgaaatgct ctccaaaata cggctcgctc acttcgagga aggcctgtgt gtacaaacca
    16141 tgcatatcgg cccttacgcc accgaacctg ccaccatcga tagaatgaaa gaatacatgg
    16201 ctgccaacaa gttgaaggac aatgtcggcc caaatggcat gcatcacgag atttacttga
    16261 gcgatccacg gaaagcgtct cctgacaaaa tgaaaaccgt attaaggcat ccagtaattg
    16321 cgctataatt tcgtttgcaa aaaacttcac aaaagaagtg gaaacgaatt gaaatgaaaa
    16381 acagattcgc gttatttata ctcattgcag ttttgatcgg cagctgcgcc cctacaccat
    16441 cgaacactgc aactgcttca ctaccatcat ccactgtaat tgcagattcc cctactgcaa
    16501 cgatcgcgcc ctcggcaact ccgaccccag tgccaataac ccttcctgtt gatcgtttca
    16561 acccgatccc tgaacttaac ccaattacga cagaaaatat cgacgctctg gaagaagtgg
    16621 ctttttttga caattctccc atatccacac acattacaga agacaaaagc aggttgaccg
    16681 tctggtttga atctggctta caaacttttg atatgccaac actgacacca tatcccttta
    16741 tcaggatgaa tatgtccatt tttagttcta atgatgcgat ctctgaagat ggacggcttt
    16801 ttgccagggc atacccggga gtcaaagccg aattctggac gcccaccatt tttatctggg
    16861 atacagaaac aggggaagag gtatgcaaaa tcaaactatc agagcatcga gggaattttt
    16921 tctacacaaa ttttcacttg gaacagaata cattttcaat tcatggagac ctgggtccca
    16981 atggaaagca taaagtcatc atctacgggc tgaatacttg tcaaaggata tttgaccggg
    17041 attctgattc agccatgtct gcgatctcac cagatggaaa atatgctgca gttactcaaa
    17101 aggggcaggt tgttatttac gacacgagca ccaaaagcca taacgccatt ggagaaacaa
    17161 ggggagccca gggagtggct ttcttgcccg atagcggaga agttgccatc tcctattccg
    17221 ggcaaacttt tctgtacgac atgaccagcg gagaggtgaa tgggttcttc gatgcaggta
    17281 tatcgaattt ttattcaaca atttccgttt tagatcacgg tgaatgggtt gttatctcag
    17341 cttccgaaaa gaattacttc tggaattcat ctgaaaaaca ggtgtattcc gtacaggaat
    17401 tcatagggcg cgattttaaa ttcagcaacg gcatcctgac cacctacaaa accgttttca
    17461 acttaagaac caaggcaaaa atcagcatcg aaaattacgg acctcaggct tttacggcgc
    17521 tttcatccga tcagagctac cttgccgtca gttcaaatat tccacccttt gtaaccgata
    17581 tttacgagac cgctacaggc aaacagatca actcaattaa aggggtatat ggtccgatca
    17641 ccgtaggctc cgattcattt atagcaagca gccacgaagt tgggaaggtt ttcaacttta
    17701 ctaccggaga aaccgatcaa gatcttgacc gcagctacct gagcggcttc cttttgggtg
    17761 aaaaggaaat cgtgacttgg gatgcactag gcaaaatttc cctgctagac ataaaaacag
    17821 gcaaggctgt gacagcggca aacctgcccg tctttccctc aagtttccta aattacacac
    17881 gatacaaaaa tgcaacgcca ttatgggatg aactatcaca gtcagatttt gccagcctgc
    17941 cgccttcaaa caccatggtt tcaaacgatc ggaccatagg gatccaggaa aataatggac
    18001 agctacagat tttcgccctt tcagatggga atttcatccc agcaactgac gatccgattg
    18061 ctgcttacaa tctgacaaat cttggttttc aattcaagtt cagcccggac gatacgatca
    18121 tagcgggggc aaattacaac tcattaactt tgtgggatgg ccaaaccgga gatcagttgg
    18181 aaagaatcgg actccccaat ggcgccgtca cggacatacg gttttcagcc gacggcacaa
    18241 agatcgtcat cagcaagaac gatgggccgc ctcaaataaa tgcatttgga aatctgtcct
    18301 tgatcatttt tgatgttctt gagaaaaaaa tcgttcagga ttacaaatta aatcagcagc
    18361 taaataaaac aggctgcaac acaacattac cctttgcatt gacatcagac ggaggccgag
    18421 tcgccacatt gacccaggat tgcaagatag gagtttacga cagtctcacg tttgaagagc
    18481 tttcatcctt cgggtcacca tattcagatt caaaccctgc tttttcaatc tcgccggata
    18541 acaacctgct ggttgtagtt gacagaaaaa acattgaaat ttgggatata aaaaccgaaa
    18601 aaagaataag aaagatggac attcctgaac ttgcaggaac atttgatttc tatgctcaca
    18661 aggctgcttt tacatctgat ggtaagatga tcattgtcaa ttcaaccttt tggagccaaa
    18721 gttctgtaac aacattttgg gcagttccaa gcatacaata atattttccc ttaaaatttg
    18781 aaaaaggaaa caaacagcgc catgaactgt ttttaccacc cagatcaacc tgctgtcggc
    18841 ttgtgtaaat actgccagcg cggactttgc ctcaactgtg ccactgttgt agatgatgta
    18901 ctagcctgca aagaccgtca tgaagacgaa gtccatcaac tggaacaact gacagcacgg
    18961 aacttattcc agtccaaacg tgtagcgtca ggctatatgc gaaatgctat tttttatggc
    19021 ctggttgggg ctgcctttac aggttttggg ctatggcaat tgaaatggtt gggactgcaa
    19081 gctgttgttt tcataatgct gggtgtattc ctgttctatg cggcaggtgc aaactatctc
    19141 gaagcgagaa agcacaaata aaatgggaac ctggatctgt cacctccgta tcgccgaaaa
    19201 actactaact cacttcccag aactcgacaa aaccctcttt acctttggta acctcgcccc
    19261 agattcgggc atccccaacg aaacgtggac ggaatttgac ccgcccaaag aagtgacaca
    19321 tttcctgcaa aaaggcgaag gcgaagacct gattcgcgat ttggtatatt atcgtgaata
    19381 tcttgcgaat cttcatttgt atgatgatcc acaaaaatat agtttccgtc tcgggtattt
    19441 tacccacctt atctgcgata tcctctggat gaaatacatc ggctcaacaa caaagattgc
    19501 attccacgaa ttatttgaac aggataaaca aaaggctgtc gatcttgtca aaaatgactg
    19561 gtacggactc gatcaactct acaatcgtgc gcaccccgaa aacctgttct ggcaggtcat
    19621 ttacaaaaca ccaaacccgc catcctacct gccattcgtt caggaaaaag ccctgcacca
    19681 acagtacgcg cacatccgta aattttacgg cgaaccagac tctgaatggt ttattgaacg
    19741 cccctacccc tatctaagcg agtcaagcat gacgcgtgtt gttgaagaca ctgttcaagc
    19801 cactcttatt attttggaaa aaatcaagta cactgacatc aataaaactc aaagttcaac
    19861 tgaaatcctc ccacaggagt ttatgcgtcc ctactcaaca cccctctaaa ttatcgtttt
    19921 cttaaatcca ttttacaggt acatgaacga attcgagaat tttttcaacc tctccaccga
    19981 tcccatgacc attgcagatg caaatgggaa aattattcat gcgaacagcg tctacacagc
    20041 cattacaggg tggagcgtgg acgaactgaa agcaacctct tattgggatc tgcttcatcc
    20101 cgatgatcac ccaaaggcac gcgccgcatt acaaactgtc aaagctggcc accctgtttt
    20161 cgccctggaa tacagattcc tctgcaaaga cggcacatat aaaaccttta tcgcaaacct
    20221 cagtggaaat aaaaaaagcg aacatcttta cgccatcagc aggctaaagg aaggacagat
    20281 cgttccatat atcatggttg caaatgccgc accggtagct gtgatcatta tcaacaccag
    20341 cggcacgatc attcatgcca acgatctggc aaccaccttg tttggatacg aagcggggga
    20401 attacaggaa aaatccattg aagatctaat acccatgcga ttaagaaaaa aacatcaagc
    20461 ccaccgcgga gattatcaat ccaacccggt caaccgccca atgggctcgc attacaacct
    20521 gaagggtttg caaaaaaacg gactggaaat tcgggtggat attgcattga accccctgta
    20581 cgagcaaaac gaactatatg ttgcctgctc cattctcgat atcaccgaaa agatcaccac
    20641 cgccgaactc gccctaaacc ttgaaaggga aaacctgcgc ctcgcccgcc ttgcccaacg
    20701 cgatccgctc acgcaggcct acaaccgccg caccatggaa gaaatgtttc caaagatcgc
    20761 ggaagaatgc aaaaagcaaa acaggagtgt ctctgcaatt atgctggatt tggataattt
    20821 caaaacattc aacgacagct acggacatca aacaggtgac caactgctaa aagagcttgc
    20881 ctacatcgcc caaaaccaca taagggaaaa tgatattctt gtcagatacg gcggcgaaga
    20941 attcttcatc atcatgccaa attgcacccc gcatcaggcc agcgaagtgg cggaacggat
    21001 tcgatcttcg ctggaaaaag acatgacctc aggccacaaa atcacagcca gcctcgggat
    21061 cagcacctat ggtttttcaa acggccccgg cgagcctctg gatgaaattt tggaaaaact
    21121 cattgaacaa tccgatcagg cactatatca ggccaaggac aagggcaaaa accgcgtaca
    21181 acatttttcc tctatttaat ttgcaactct ttttatttgc ctgcgtaaag caagtagaca
    21241 aacgaaagga gtaaaaatga acgaagtcaa aaccctcacc cgaagcaaat ccaaccgcat
    21301 gatcgcgggc gtgtgcgcag gccttgcaga ttatctcaac atggacccca ccgttgtgcg
    21361 tttattattt gtgattggtt tcttcacgtt gaacggcggc atgctggtgg cctatttgat
    21421 catggccatc gtcacccccg agcaataaaa caacagggac acggcatgcc gtgtccctgt
    21481 gattatcgag ccagtgagag gaagacatcc tccagagttg cctcgcctct ttcgattttt
    21541 tccacccgca ggctttttgc acttaacagg ctttgcatcg cctctttttc gacctgtgag
    21601 ctgatcaccc gtaaatgatc acccgccaac ccgacccgtt ccacaccctg aacggaagaa
    21661 agtatctcca atcccttttc aagcgggtaa gcatacactt cccacacatc tcctgtaatg
    21721 accgaatgtt tcagcgcggt cggtgtatcc atcgccagta attttccatc gcgcatcacc
    21781 cccacccgtt cgcaatattc agcttcatcc atgtaatgcg tggtgacgag gatcgtcaca
    21841 ccgctttccg ccaattcata gatcaaatcc caaaacgcac gacgggcagt ggggtctaca
    21901 ccagaagtgg gttcatcaag gaacaacaat ttcggctcgt ggaccagcgc gatgccaagt
    21961 gacaaccgct gccgccatcc tgtagacagg tctctggtga gggtatgttc atgcccattc
    22021 aggccgacca gttcaagggt ccgttggatg cgggttttgt cggtgatccc ataaacgccg
    22081 ccgtaaaaag tcaggttttc cagagtcgtc aagtcatcat agatcgcgaa tttttgcgac
    22141 atatacccaa cacgcatacg gatttcttcg ctttccttga agacatcata accaaggaca
    22201 gtggcctttc cgtcgctggg tttgagcaaa cccaataaca tgcgaatggt ggtggtcttt
    22261 cccgagccgt tcgggccaag atacccgacc acctcacccg cctgcacttc aaagttgatg
    22321 tgatccacgg caatgaaatc gccgaagcga cgggtgaggt tttgaacggt gattacagcc
    22381 tcagtcattg ctcacctccg ataaagcaat aaatacatct tccaatgcag gtggaatgat
    22441 acgcaggtcg gtcagtgtgc cgccagctgc tttgatctcc tcaggcaggc gtttcataat
    22501 gaggtcagcc tgcccttcca acacacgaac atgtaatttg tcgccaaaag cggccacatc
    22561 ctccacacca gaatcttgat gggcaacatg ccgcagtgtg ttgatggggt tgccgcgcaa
    22621 ttcaagaatg cggccattaa gccgtgaccg caaactggac ggggtatctt cagcgatgat
    22681 ttttcctttt ttcataaacc caacccgatg acagcgtgaa gcttcatcca tgtaaggggt
    22741 ggagatgatg acggacaccc cttcagattg caaagtttca ggctgcgaac tgacgggctg
    22801 ggaaaccagc ttgatcacca attgccaaaa gtcctgacgg gtaacgggat ccacccctgt
    22861 ggtgggttca tcaagcaaaa gcaatcgcgg acgtgtgaca agcgcagaag ccagcccaag
    22921 tttctgcttc ataccgcccg agagctgccc agcgcggcgg tctttgaatt tttcgaggcc
    22981 gacgaattca aggatctcca ttgaacgcgg caaccattct gtagtggaga gcccacggat
    23041 ctcggcaaag aagcggatgt tctcaagaac ggtaaggtct tcgtacatgg aaaaacgctg
    23101 cgaaagataa ccgatcgtgg agcgggcttg ttcagcctgc ttgagtacat cgtatccgca
    23161 aatggtgatc tctccctggc tgggcaacaa tgcaccaaca agcagacgca tggtggtggt
    23221 cttccccgcg ccatctgaac cgacaaggcc atagatctca ccaggtttga tcttcaaatc
    23281 aaccccatcc actgcgtgaa cactaccaaa gtgcctcacc aatccatttg cctgaaccaa
    23341 ataatcattc ataaaaatct gcctttaatt tattttgcac atgtcttgcg ggtacatcct
    23401 gacgccagaa tttatttgaa aaccacatcg gcgggcatgc cgatcttcaa cttgccatca
    23461 gggtcagata cctgtaattt gatggcgtaa aaagtggagc tgcgtccttc cacggtttgg
    23521 acattgcgag gggtgaactc cgcctgatcg gcaatgtgaa caacagtggc agtgaaggta
    23581 agcccgggga aagaatccac gctgacttca gccgtttgcc caaggctgac ctgcccatag
    23641 cgatcttcag ggacataaac ggtgatggtc aggttggtga gatctgccat cgtcagagca
    23701 actgctccag gctgcacaaa ttcaccaggc tccacattgc gggagaggat gactccgctc
    23761 atgaccgcat aaacatccaa ttttgcaatt tgcgcatcaa gcaggtcgag gttggcttgc
    23821 gcttgatcta cggcggtctg ggcctgatcg agcgtgttct tagctgaaat cacagcagga
    23881 gactccgtgc cagtttgcag ggagagcagg cgggtgtagg ccgcatcata acgctgctgg
    23941 gcaatcacca cctgtccacg ggcatatttg atgtcatctt ctgcttcagt attggtgagg
    24001 gcattgaagt catcctgtgc gcgttgcagt tcatcaagga catcgttgta gtaatcgtag
    24061 gcggcatcct ggacggcacc agcttcaaca gcattttcag cctgcacctt gaccttatct
    24121 gcaatgagga aggcggcgcg tgcctcagag agtttcttct cggcatcaag atactgtgca
    24181 ttgcccagat cagagatcac tttttcaagg ttggcattcg cttcatcgag agcgatcttt
    24241 gcggcatcca cctcggtttg agcggcggcg agttggtctg tctggtcaat aaaccataaa
    24301 ggttgattga attcatcagg tgcagaaact cgcaggtcct ttgcgcgttg ggcatcttgc
    24361 gcagagagag cggcctgcag ggtttggtca aatttggttt gggcggttgt cagggcggct
    24421 ttggcagaat caagcgcgga ggcggccact gcccgttggg cagtcagcag gctggggtcg
    24481 aggtgcagaa gcggatcgtt cgcatttacc aatgcgcctt cggccacatc cactccggtg
    24541 acttttccag caagttcggg tgaaacgttc acaatgacag cttcaatgga acctgatgcg
    24601 gtaagttcgc cgtttccatt ggtgttaagt gtgaggaatg caagataacc gatggcacca
    24661 agtacaacaa gagccaggac gatacgaacg ggtacgggaa ttgatttgtg attcatggtt
    24721 taactccaat gttttaataa aaagactgat tacgagatcc tctacattaa tcgaggcgtt
    24781 tgcggaagcg gagtgcggcg gcacccatga tgactgagcc aaatatgacc agtgcaatta
    24841 cttcgttttg aatggccacc aatccgactc cctttatcaa gagtgagcga atgatgatga
    24901 ggtaataacg aagcggtatg gcataggaaa cgatctgcaa aaatctcggc atggccgcaa
    24961 tgggaaaaaa gaaaccagaa aggaagatca tgggcagatt gaacatcatc acggtcatca
    25021 tggcttcctg ttgagtgttg gccatggtcg aggcaaacaa cccaattcca agactcgaca
    25081 tgaggaacag cccggatgtg acgatgatca gcacagcgct tccacggatg ggcacgccaa
    25141 accaccaatg cccgatcaac aagacctcaa aggtttcgac aaaacccaaa ataacgtaag
    25201 gcagcaattt accggcgatc agctcccacg aacggatggg ggttacgatc aactgttcaa
    25261 tggtgccgcg ttcgcgttcg cgcacgatgg tggtggcggt aaggatggaa gtgatgaagg
    25321 aaaggatcat gccgatcaca ccgggaatgt taaagtaggc tccatccagg tctgggttgt
    25381 accaaacctg cgtgcggact tccaaaggtg cggagggtgc tccacgcccg ctttgcaggg
    25441 tttgttcact gaggatcttc gtggagtagg cctgcccaac aagccgagcc gtggaaagtg
    25501 cagttgcgcc aacactggca tctgaaccat ccagcaccat gagtacttgt gcgtttcctt
    25561 caatcaaacg gcggtcgtaa tcggctggga tgaccagtac aacccgtgta tcacccgact
    25621 cgatcaatgc ctgatattca tccgttgagc cgactgaata ttcaaggatg aagtaatcgg
    25681 cagcgcgaaa agcatccagc agggcacggg attgcgtggt cttgctttgg tcccaaaccg
    25741 ccatggaaat attttttacg tcggtggttg ccgcataacc cagcaggaac aactccataa
    25801 tgggcatgat gatgatcatt gccagggtgc gcggatcacg aaggatttgc aaaaactcct
    25861 tgcgaataat ggaagagatg cgggaattga gcataaaagt ttcctagctt tctggtttca
    25921 agataccgtg taaatagaca tcaacataca catccatggg gtttttgggc atgaggctgc
    25981 ccacgatcga attggagaca accatctctg taataaagta ggagaccacc aacccaaaaa
    26041 aggaacgcat cagcatggca gggttggtca cacgcaggtt tttcctgatc ttcacgatct
    26101 gttcgaacac aggcagcacc ttgggagcaa cttccttcaa catggccgcc ccatgccccc
    26161 cattgaattc aacaaactcg atcatcatca gcttgatgta gtacggctcc ttgctgagtt
    26221 cggtgatcgc aatccgcatg gcgttgcgca ggaattcctc ggcggtatcc ccttttgcct
    26281 ccaggatcaa cggcaatatc tttttataag gatgtttgtc gataatgatg ccttcgaaaa
    26341 tttcctcttt actggcaaag tggttgtaaa ttccaccaag tgccagcccc gcctgatcgg
    26401 caatctgccg catggatgtg gcgtgatagc catgctccat gaataactca atggcggcat
    26461 cttcaatggc aaggcgggtg acctcgcctt ttttaggtgc atcttttgat ttcgctgaca
    26521 tggcttcctt tcaagaccaa aaatgaacga acgttcgttt ttattttatg caaaagtaga
    26581 aatctgtcaa taggcaaatt gcatcgggta aaatcatcta catgcttccc tttaccacac
    26641 ttgaaaaaca gattcgtgca gacctgaaat acaacatcaa ggtcaatctt tgggatggcg
    26701 cattattcgg tgttgcactt ggctttgcct cctttggaac cgtgctgcca ctcttcgtcg
    26761 cgtccatgac caactccgcc ctgcttattg gtcttgtgcc tgccattcat tcagttggct
    26821 ggcaattgcc gcaacttttt accgccagtt atgtttcgcg cttgaggcgc tacaaacgca
    26881 gtgtaatgat gatgaccatc catgaacggg ttccattttt agggtttgga atcatcgcgc
    26941 tcctgctgcc catgattggg ttgaaggtcg ggcttgtcat cacttttctt ttgctcatct
    27001 ggcaggggct tggcggcgga ttcactgcca actcgtggac cagcatgatc tcgaagatca
    27061 ttccgcctga aaaccgcggc accttctttg gatttcaagc agggctcgca aacctcttca
    27121 tcagcggatc agccgtggcg gcaggttatc ttttagacta cctcgattca cccctggact
    27181 ttgcggcctg cttcttcatc gcaagcatct ttttcacctt gtcatggttc gcacttgccc
    27241 tcacacgaga acccgaagat accgaaaaga tcatccctga agaaaagacc cacttctggg
    27301 acgattcgaa aaaaatcctg aaaaaagatt tcaatttcaa ctggttcctc gttgcaagat
    27361 tcatttccca atttgcaacg atgggatttt ccttctacat catttacgcc ctccgccgct
    27421 tcaacatgga tgccatcaca gcgggatacc tcaccgccac gctcaccatc gcccaaacca
    27481 ttgccaatgc cagcatgggc tggctcggtg acaaatgggg tcaccgttcc atgctcatca
    27541 ttggggctgc ggctgcgttg gcaagtgcca cccttgcatg gctggcaaca tccattttct
    27601 ggttctatcc catcttcctt ctcacaggat tcgccaacgt atccatctgg accattggaa
    27661 tggcgatgac cgtggatttt ggcaccgaag ccgaaagacc gttgtacatc ggcctttcgc
    27721 aaaccctcac tgccccagcc accattctcg ctcccctgct cggcggttgg atcgtggatg
    27781 cggcaggctt catcccgacg ttttcaattt ccatcattct atccatcgcc atgatcggaa
    27841 ttctggtctt ccttgtgaaa gacccgcgca tgcatcactc aagaaagatg aaggaagtga
    27901 aatagttttc tttcatccca tatcaatttt caccatttta cgggagcctc catgtcactc
    27961 ataaaagaaa aagtaaatca agccattgaa atcctcaaag aacaacagat cgatatgtgg
    28021 ttgaccttcg tccgcgagac cagcggtgta cgcgaccctg cccttgattt cctcatcggc
    28081 gaaaacgacc tcacctggcc ttcggcgttg atcctcactc gcaaaggcga aaagatcgcc
    28141 atcattggca acctcgaaaa agaatctctg caaaggctcg atgtctttga cgagattctc
    28201 ggatacgaca ccgccgtcag cacattgttc aaagagacga tcacccgcct caaccctgac
    28261 cgtattgcgg ttaacacctc ccgaaacaac gtccacgcag acggattgac ccatgccatg
    28321 tacgaattcc tcagggaata tctggcaggc acgccctatg ctgaccgtct tgtcagtgcc
    28381 gagccagtca tctctgccat gcgcggacgc aaaaccgcca cagagcaggc ccgtgtcaaa
    28441 aaagccattg agatcacaga agaaattttc gccaagacct ttgatttcat caaagtcggt
    28501 atgaccgaga tcgaagttgg cgcatacatg catcaactgg ccaaagacta caacgtggac
    28561 cttgcctggc ctgctgaaaa ttgtcctgct gtcaactcag ggccgaattc tcccgtcggg
    28621 cacaatggcc ccaccgatat caagatcgaa cgcggacatc ttgtccattt tgattttggc
    28681 gtaaagtacg aagggtattg ctccgatatt caacgcattg cctatgtgct gcgcgaagga
    28741 gaaaccgaag cccccatcga aattcaacgc ggattcatca ccatccgcac cgcgatcgag
    28801 aaatcgcgtg aggcgatgaa acctggagtg acaggcaatg tcattgacac catctcacgt
    28861 gagatcgttg ccgatgcagg ctaccccgat tacaaatatg cccttgggca tcaacttggg
    28921 cgtgtggctc acgatggcgg cgcattgctg ggtccgctct gggaaaaata tggcgactcg
    28981 cccaatcaaa aactggagat cggtcagatc ttcacgatcg agcctggctt gcctgtaaac
    29041 ggctacggct atcttggcct tgaggaagat gtggtcatga ccaaaagcgg cgcggagtat
    29101 tttcacgaac cgcaaaaaga gatcgttttg ttgaagggat aagagaatga agcgcgagag
    29161 agtatctcgc gcttttgatt cggagagaaa aatgtcactt aatcagagtc aggacagggg
    29221 ttggaatgaa gcggttcttc atcccaattg tatgacctcc atcactggca gttaagtcgc
    29281 gtaatgctat acttctgatt gtgaaattta tcaccaaaag gatgaaatat gactcaggaa
    29341 gatcccagaa ttttggaatt gagaaagatg cgcgccaagg ctttggaagg tggcggcgag
    29401 gaacgaattg ccaaacaaaa agccaaaggc aaaatgacag cgcgcgaacg tgtgattgcc
    29461 ctgctcgacc ctggcacatt caatgagatc gaaccgttta ttacacatca aggcgatgaa
    29521 ttggatctgc tgaaggataa gttttatggc gatggcgtca tcacggggta tggtcagatc
    29581 aacgggcgga cggtttattt gtattcacag gatttcacaa tttatggcgg cacactcagt
    29641 gaaatgcaat cgcacaaaat ttgccgtgta atggatctgg cggtacgcaa tggcgtgccg
    29701 ttcatttcgt tgatcgactc aggcggtgcg cgcattcagg aaggtgtacg ttccatgggc
    29761 ggctatgccg agattttccg acgcaacgca caatactcag gcgtggtgcc gcagatcagt
    29821 gtgatgcttg ggccttgcgc aggcggcgcg gcctactccc ctgccctcac cgatctggtc
    29881 atcatggtcg aaaaacaatc cttcatgttc ctgactgggc ctgaagtgat caaagctgtg
    29941 acaggtgaga tgatcgacgc agagtcactg ggcggttcgg gtgtgcatac atccgtaagc
    30001 ggtgtggccc atctgagcgt acaaagtgaa aaagcggcgc ttgaaatggc gcgccacttc
    30061 ctttcgtacc tgccttcgaa caatgtggaa aatccgccgt tcaccccaac gaccgatgac
    30121 ctggcccgca tggatgaaga actgaatcac atgattccgc tcgaagccac tgagccgtat
    30181 gtgatgcacc aggtgatcga aaagatcatt gaccaggggt cattccttga aatccaaccc
    30241 gattgggcgc ggaatgccat tgtaggcctc gcccgcatcg gcggacacag tgtgggcatt
    30301 gtcgggcagg aacctgccat catggcaggc gtgatcgata tcgatgccgc cgacaaaatg
    30361 acccgcttcg tgcgcatgtg cgactgtttc aatattccgc tcgtcacgtt tgttgactca
    30421 cctggttttc tgccaggcat cgcacaggaa catggcggca tcattcgtca tggcgcaaaa
    30481 cttttatacg cctattcaga agccaccgta ccgaagatct gcatcatcac ccgcaaagca
    30541 tacggcgggg catacgttgt catgtccagc aagtatctcg gcaccgatgt cacctatgca
    30601 tggccttcgg ctgagatcgc cgtgttgggc gcggaaggcg cagcgaatat tttgttcaag
    30661 aagcagatcg aaaccgcaaa tgacccaacc gcggaacgca aaaaacttgc gaatgaatac
    30721 cgcgaaaaat tcaacaatcc gtactatgcc gcttccacag gctatgtgga cgacatcatc
    30781 gaaccgcgtg aaagccgccc gaaactcatc gcaaccctgt ctgcactccg cgacaagtac
    30841 gcacccgcac cgccgcgcaa gcatgggaat atccctgtgt agaaaacaga aagtggaaag
    30901 caaattcatt actttccact ttcaaggcaa atacccatga ccactgatct actcctctcc
    30961 cttcaaatca ccgcccttgg tatggggctt gtgttcggcg cgatcctttt attgtggctg
    31021 atgatggccc tgttgacccg cttcaccgca gaaaaagaat cagcggctga aacagactcc
    31081 accgcacaaa caggcgcatc tgactcaccc gaacctgctc ccgtttcaac aagcggattc
    31141 aaagcccagg ccgccgcact ggctgtggcg ctggcgctcg ccgaacagga actatcttca
    31201 gcgcatccgc tgcttgaacc gcccacggcc attgtctctg cctggcaatt gggcatgcgg
    31261 accagtcaac gctctgaaaa aggaaatttt agaagacact aaccagacct gaaaggtctt
    31321 cggagaatgt atgaaatata aagtcacagt caacaatcag gtttatgaag ttgagatcga
    31381 aaacatcaac actcgccccg tcattgcgca cgtggacggt gaacggtttg agatcatgcc
    31441 tgagaatgct ggtcaggccg aaacaggaaa agactcgagc atcccaactg agaaaaaagc
    31501 ctttaacccg tccccagtca cagccgcaaa accatctccc aaccccgcat tgagcggaaa
    31561 tatcctcacc gcgccgttgc ctggaaccat tgtagaggtt tttgtgaagc ctggtgaaaa
    31621 agtggaagct gggcaggtgg ttgtgatcat cgaggcgatg aaaatgaaga acagcatccg
    31681 ctcggtatac agcggcacgg tgaacgaagt gttcgtgagt gcaggccaaa gtgtggcgca
    31741 caaacaagcg ctggtcaaat tcgcagacct gggcgaggcc tcgtggatgt aacaatgttt
    31801 acaggttgca agtttcaggt ttaaggattc ttatggatct aacttcccta cttcccgaac
    31861 tactaaaagg ttttacccat ttcaccctcg gcaatggggt gatgatcttc gccgcgctga
    31921 ttctggtgta tttggctgta ttcaaagaga tcgaacccgt cttgttgctg ccgatcggtt
    31981 ttgggtgctt gcttgcaaat attccgctgg caggcatgac cgccgcagaa ggcatgtcaa
    32041 aagttttgta cgatgcaggc attaagacag aattattccc cctgttgatt ttcatcggcg
    32101 tgggagcaat gatcgacttt tcaccgctgc tcgctcaacc gcgcatggtt ctgctcggcg
    32161 ccgcaggtca atttggaatt tttggcacac tgatgctggc aattgcactg ggatttccgc
    32221 tgaaccaggc agcatccatc ggtgtcattg gcgccattga cggaccaact tcgatttttg
    32281 ttgcgaccaa actcgctcct gaactgctcg cgccgatcgc tgtggcggca tattcataca
    32341 tgagtttgat ccccatcatc caaccgccgc tgatgaaact actcacaaca aaaaaggaac
    32401 gcatgatcca aatggattat gcgccgacac cgatctcgaa aaagacgttg atcttctttc
    32461 ctgttgtgct gaccattgtt gtcggcctgc ttgtgccaga agccaccccg ttgatctcga
    32521 tgctgatgct tggaaattta ctgaaagtat caggcgtagt ggaccgtttg agcaatgccg
    32581 ctcaaaatga gatcatcaat attgcgacat tatttttggg tctggcaatc ggctccacca
    32641 tgagcgcagc cagtttcctc aatctcgata caggcaagat catgatcctc ggcctgttcg
    32701 cttttgcgct ggatacggtg gctgggctgt tgtttggaaa actgatggcg gtcgtcacag
    32761 gcgggaagat caatccgctc atcggcgcgg ctggaatttc cgcgtttccg atggcaggac
    32821 gcctcgctgc caaaactgcc aacgacgaag acccaaacaa tttcattctc atgcatgcga
    32881 tgggagcaaa cacagcaggc cagcttggaa gcgtgatcgc gggcggtatt ctgctctcgg
    32941 t
//
LOCUS       DAOQJC010000039        32797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_596_length_32797_cov_8.785994, whole genome shotgun sequence.
ACCESSION   DAOQJC010000039 DAOQJC010000000
VERSION     DAOQJC010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 32797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32797
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_596_length_32797_cov_8.785994"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            337..1236
                     /locus_tag="PLE39_07870"
     CDS             337..1236
                     /locus_tag="PLE39_07870"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNE68282.1"
                     /translation="MDFSWISNPESWIALVTLIALELVLGVDNVIFISILSGKLPQED
                     QQRARTTGISLAVITRVLLLLSLSWIIGLTEPLFHLPFFEGEVAEITGRDLILLLGGL
                     FLLWKSTHEIHDKLEGKEGSASAKVAANFVSVIIQIMLLDIVFSLDSVITAVGIADNI
                     IIMIVAVVAAAVVMIFTAKPIGDFVERHPTIKILALSFLLMIGFTLIAEGFGVEIPKG
                     YVYFAMGFSVMVEMLNLRVRDSHVKPVNLRDPYKEEKVELQPAFVAASKAATVTTEKL
                     TTSPAKKASTKTKAKPKSKTKKS"
     gene            complement(1280..2164)
                     /gene="thrB"
                     /locus_tag="PLE39_07875"
     CDS             complement(1280..2164)
                     /gene="thrB"
                     /locus_tag="PLE39_07875"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNE68283.1"
                     /translation="MLKIRVPATSANLGPGFDCLGLALDLWNEITFEPAEKLTYHVTG
                     EGAEKLNKGTKNLLTKSYSNLYEFCGKKVSPVRICAHNEILMSSGLGSSAAAIVSGLF
                     GANEMLGSPLSQNELLKFASDMEGHPDNVAPALFGGLVVSVMAGEEIISHRYEVPELT
                     IVIVKPIVEWLTKTARAVLPKSVSRADAVYNIGRTSLVVEALRTGNLALLQKVMDDRI
                     HQPYRLRHISGGTAAYKKARQFGAAALSGAGPSIITFVPPDHAELARSEIESVFVERG
                     IETKSVITKPGKQGAHRV"
     gene            2261..3295
                     /gene="thrC"
                     /locus_tag="PLE39_07880"
     CDS             2261..3295
                     /gene="thrC"
                     /locus_tag="PLE39_07880"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNE68284.1"
                     /translation="MTYQGLIHRYGQYLDLPAHTKTISLLEGNTPLIPMPRLGRELGC
                     ELFIKFEGLNPTGSFKDRGMTAAISEAVGRGAQTVICASTGNTAASAAAYAARAGLRS
                     IVLIPEGKVAVGKLAGAIAYGAEVIQIQGSFDDALTLVVEITQKTPIALVNSINPFRI
                     EGQKTASFEICDDLGSAPDWLCLPVGNAGNITSYWAGFKQYKKGLPQVLGVQAAGAAP
                     LVLGHPIEKPETIATAIRIGKPARGEQALQAAEESNGRIIAVSDEQILSTQKILAAEG
                     VWVEPASAAGLAGLLAESASGKFEAKGKRIVVVCTGHGMKDPSIITESFKSPKVISAD
                     YDALISAVGA"
     gene            3383..4387
                     /locus_tag="PLE39_07885"
     CDS             3383..4387
                     /locus_tag="PLE39_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68285.1"
                     /translation="MFFNEWNILVVDDEPDVLAVTKLALRDVKVYGLPVKIHTASSKA
                     EAIEMLKGPLALQGSTEPIVAVALVDVVMENDQAGLELCKFIREEANGHSVQLFIRTG
                     QPGVAPERSVIDNYDISGYFTKVELSEQKLYTLVKSGVRQWFTSWYALMTEESTNNIV
                     THSGSRKDFLENGIGWFGEPGPQEGEGVTGFIFDRNLIVSDYPTQIKPLYDRLNALKP
                     VIETAEGHKLAMDEMGNLLVKTVKTKTTADYAYVGESSMVMPRLLLELTFKSGLVFST
                     LWKKITDDEKRAAAKKPVAKKSAKKPAKPAKKAVKKVAKKVAVKPAKKTTKKAAKKKK
                     "
     gene            4492..5700
                     /locus_tag="PLE39_07890"
     CDS             4492..5700
                     /locus_tag="PLE39_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE68286.1"
                     /translation="MTKKNPVLNPNILQKFSFFASFSKDQLEEIAKSAPKVACKTGEI
                     VFRQGDRSATMYLILKGAVKIEREDSVGGIVSVGQLSVHQVFGELAMLSKEPRQATVT
                     VTKNAEFLVIDRKMMLEIIAKSDPEEIIEVFSVLSEQTRAANDREFHETLSRRTLEAQ
                     MEVEKQRALTQLVAGVAHEINTPLGIINTAVSIMARELSEPSEITAQRAADIAESLEL
                     MRRNVERAHRLVQDFKKVSASQLMDEIETFDISETIRETVDLMMVGLKSSQIQAKFHD
                     KLTLTQKAWMGYRGGLSQILINLLTNAERHAYPNGAGGLIDITIEMFDEKNYRLTVQD
                     YGQGIPAENQPRIFEPFFTTARARGGTGLGLAIIHNLVVNVLKGEIQLRSEIQKGTAF
                     LVVFPRDLPK"
     gene            5818..7461
                     /locus_tag="PLE39_07895"
     CDS             5818..7461
                     /locus_tag="PLE39_07895"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68287.1"
                     /translation="MNEIPLVVENLTFKYRVRPELAIENISFELKQGELLLIAGSSGC
                     GKTTLARCINGLVPRSYRGERSGRVLLHGKEVTELKIAEMAQVVGTLLQDPERQIVAS
                     NVYNEIAFGPENLGLPHDEIEARIEQALKRLKIEYLRDRETFNLSGGEKQKVALAGLL
                     AMNPSILLLDEPLASLDPASAHDALKIFRSLADEGKTIILVEHRVEDSIEAKPDRLLY
                     MESGRIKYLGGITSLPKEIDHTQVKLPAQWVVQRVKEDKKHADVPPVQKGESGDEPLV
                     VFEDVSFRYDDEYPLILKDVNLTINRGDLIAVLGPNGAGKSTLVKHAIGLLKPTSGRV
                     LVQGEDTRKMSVAQIARVLGFVFQSPTHMLYAPTVQEELEFGPKNLAFDKEAMDKSVT
                     ESLNAVNLKGFEEYPPLGLSFGQQKRTTIAAVLAMQSKIMVMDEPTAGQDFANYTRFM
                     DALCSASNNAKSILSANFDSTLFITHDVDLAVTYANRVLLFGDKHVVADGKPEEVLKD
                     IDLLMKYRVRPTSLLHLNLDLFAKTKKFLPAEALAGFVS"
     gene            7547..8095
                     /locus_tag="PLE39_07900"
     CDS             7547..8095
                     /locus_tag="PLE39_07900"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNE68288.1"
                     /translation="MNDKSTWEFGTRQVVYGAIGAALYGVLSLATNYFPLPAAGNVTF
                     RPAVAVLIFFGAAYGPWVGLLAGFIGNTLGDAAMGWGFYWNWSLGNGLMGLVAGLAMP
                     MLKDFKARGDIIKAIIYGLIGIAVGMLFASVTEKFIGGLDWNTALVGYFTPAFIGNAV
                     VVVILLPVLMIAFAAVASRRGR"
     gene            8101..8994
                     /locus_tag="PLE39_07905"
     CDS             8101..8994
                     /locus_tag="PLE39_07905"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNE68289.1"
                     /translation="MLVTWKYRPRDTFIQRLDPRTRFIYMVCIIFALTLPDIWDYRII
                     FPLFISSLTLYLLARIEWKDVRRAWIFIGVFVFFIVGLNALLSGRGGPDTVTNVYSPT
                     IFNLPLKIPFTNIGWDIPVTVAKLWFAVNQITRMLTMAILAFPIPYTVSPTIYGTTFK
                     RMGTSDKFSFTMDLAFRFLPTFARDLMITMDAQRARGYELDKLKGGIAARLRKLAPLI
                     IPVVMQSTVTGEEVIDAMDLRAFGTAPRTWLQSQDLQYKPMDYVMLSLGLAILVVCCI
                     LKWGFGIGGFFVPDFFMNLFV"
     gene            complement(9052..9879)
                     /locus_tag="PLE39_07910"
     CDS             complement(9052..9879)
                     /locus_tag="PLE39_07910"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNE68290.1"
                     /translation="MTTKTYRQLLNEWRKERDETIRRENGWLSLAGLYWLKLGKNQFG
                     ADPKCEIQLPTRISPVVGYLEYNGKSVSMRTTDGHKVHVNEKLSDFAILHPDISENPS
                     FIKFEDICLVVIQRGNRLGVRMWDNQREERRSFPARSWYDIDEQYRIPATFTPYDRPK
                     MAYFPDITGEKSEFPVEGYLSFEFGGNHYQLDINKEDDGTLFVRFWDPTSKDETYPTG
                     RYLIADIEADGKIFIDFNRAYSPPCAFTDFATCVFAPEQNHLDFKVLAGETYPRREN"
     gene            complement(9948..10541)
                     /locus_tag="PLE39_07915"
     CDS             complement(9948..10541)
                     /locus_tag="PLE39_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68291.1"
                     /translation="MKKAFGFLSNEIFLGTMIALLSAFTAIASYQGAMSDSEQNKYEI
                     LGMQTLNDGNAEYLRANQDITQDYNYFDNWYINAGERPEIAEYYQTSFSEQLAAAIER
                     DPETVWDEQYYTDMYTEATVLWDESDTNFDLASQWDERGDQLQLVMLIMALGLALAAW
                     ASLLGAESTMRTLFSLFGLLAFIAGLFIYLTMVPTVG"
     gene            10715..11269
                     /gene="dcd"
                     /locus_tag="PLE39_07920"
     CDS             10715..11269
                     /gene="dcd"
                     /locus_tag="PLE39_07920"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNE68292.1"
                     /translation="MGLKPDKWIRKMALEQKMIEPFVDSQVREGVISYGVSSYGYDVR
                     VANEFKIFTNVFSATVDPKQFDTKSMVDFVGDVCIVPPNSFALARTVEYFRIPRKVLT
                     VCLGKSTYARCGIIVNVTPFEPEWEGYVTLEISNTTPLPAKIYANEGLAQVLFFEADE
                     ECDVSYADKKGKYQKQQSIVLPKL"
     gene            11322..12593
                     /locus_tag="PLE39_07925"
     CDS             11322..12593
                     /locus_tag="PLE39_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNE68293.1"
                     /translation="MADSNEAQTMQKQMDDMNKMLLRVISHLARSTDGQTSLILRDLT
                     QISSTMAALKQSITGYLSTYERQVGALVGVGSAINSSLGLKRVLEMVMDSLIALMGAE
                     RGLLMLREPNGELKVRVARGGDREDLAQEVFSISNTVVQQVAETGNAILTTNAQDDPR
                     FGRQVSVAAYRLRSILCAPLKIKDNLIGVIYVDNRAHTGIFGNNDLSLISAFSNQAAV
                     AIDNASLFDDLQESNAELEIAYQATLEGWVRALDLRDKETEGHTQRVTALTQRLAQAM
                     GVDDDALLHITRGALLHDIGKMAIPDGILLKPGSLTDEERAHIQKHPVYAYEMLSPIK
                     FLHPALDIPYCHHEKWDGTGYPRKLKGEEIPFAARIFSVVDVWDALISDRPYRKGLPP
                     SEIRERIQKDSGSHFDPKVVETFLSLDNLAV"
     gene            complement(12702..15500)
                     /locus_tag="PLE39_07930"
     CDS             complement(12702..15500)
                     /locus_tag="PLE39_07930"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNE68294.1"
                     /translation="MTSIVSLDIETTGLDENREAIIEIAAVKFNGRRIESEFNTLINP
                     GRHIPDFITGLTGIDDAMVRQAPRLRDIANDLLAFVGDAPILGHNVKFDIGFLRKAGL
                     FEYQQTIDTYELASVLMPTASRYNLGSLGQQLNILLPATHRALDDARVTQAAYVRLFE
                     MASELPLETLNEIVQLGDFVDWDAGWVFQQALKQRAKEAPKAKRARLASFSGPMFDSA
                     KRESASIQKTEEPLPLDPEEIAAVLEYGGPFSQYFESYEHRPEQVEMLKAVTNALSTG
                     NHLMVEAGTGVGKSFAYLVPAAYFAVQNSTRVVVSTNTINLQDQLIKKDIPNLKAALN
                     LENLNATVMKGRGNYLCPRKLEYMRSHGPKDANEMRVLAKIMVWMLDNASGDRNELNL
                     TGPTEREAWARLSAEDDNCTTETCMGRMGGSCPFQRAKQAALNSHILIVNHALLLSDV
                     ASGSKVLPEYDYVIIDEAHHMESAVTGALSFRMTQNDLERMMKELGGSSAGILGRILT
                     DLHASLRPSDFGLLQQKIKRATDQAFRVEQLSKQFFMNLSDFIAIQREGQPQSNYSWQ
                     MRVMPATRTLPGWDDVEMGWGQVGETMSLLLKTLDELYKGLGDLMSEGHDSVEDIMGS
                     LSSLMRRMTEAEAASSGMMHKPSNELIYWVEVNPRGERLSLNAAPLRVGPLMQKHLWH
                     EKASVIMASATLTTHGEFQYIRNTLSAEEVDTVALGSPFDYESSTLLYVANDIPEPNI
                     HGYQQMLDKTIIATAKATGGRMLVLFTSYAALKKTAQAITGPLAREDIFVFEQGEGAS
                     PNALLESFKSTDRAVLLGTRSFWEGVDVPGDALSVVFITKLPFDVPSDPIIAARSELY
                     EDSFNEYYLPESILKFRQGFGRLIRSASDRGIVAILDRRVLTKQYGRLFLESLPQCTA
                     RQGAAAGLAKMAGQWLGT"
     gene            complement(15609..16958)
                     /locus_tag="PLE39_07935"
     CDS             complement(15609..16958)
                     /locus_tag="PLE39_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68295.1"
                     /translation="MKVSEKIGVTLLIWVILAGMAFFRLKAYGSINLSVANADTPSYI
                     DEAAPPLFSRDMLTRSRLFTTNLVYHLAKVQECELTAISHPALGLETERAVQPCFTRI
                     ALLQNLLSILAWSLLALTVASKLTGGFEKLFAAISITAFGFTPAIADWDSILGSESLT
                     FSLFAISLALLLKICFTAIEEDQRPTSILLTILTLVSLALWTFTRDSNVYALAVLFAF
                     LAVSLVIFPRLRKNKSVLVISAVILVLTVVGLQSAMESRRWKKPLTNVFAEFILPYPA
                     RAEFMTGIGMPDPKSAEYPTWFRENAPRAYARFLLAHPGYTLTSFTSNLGGIFSENTQ
                     PYFFSEQTGTRKVLNALNDILHPNTHLVFILDLLLLMGLVVSAIARRNGEFGAWAWLS
                     LWLIASASAMMVINFFADSIGVTRHTMLAVEIFRLMLWIFLAILFDQANRPLKSEKG"
     gene            complement(16983..17675)
                     /locus_tag="PLE39_07940"
     CDS             complement(16983..17675)
                     /locus_tag="PLE39_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68296.1"
                     /translation="MKIIKNEKLIERNGKIGQWMSLASLIVLGLGLYISFTVPEYFIY
                     SIVCLVIGFTMTQISIYMGNRWGRSPRADEKFDAGLKGLHSDFVIYHFSSPVSHLLIG
                     PSGAWVLHPLHQRGKISYQKNRWKLSNGGFLQAYMSVFGQEGLGRPEIDAENEVQALR
                     KFLAKKMDESTIPEIKPILVFTNDEIEVDAGDSPIAAMRLKQLKEFMRQGGKNRSLSN
                     EQIKALSDALES"
     gene            complement(17676..18692)
                     /gene="plsX"
                     /locus_tag="PLE39_07945"
     CDS             complement(17676..18692)
                     /gene="plsX"
                     /locus_tag="PLE39_07945"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNE68297.1"
                     /translation="MARIVVDAMGSDSYPVPDVEGAVMAAREYGAEIILVGDEARIKP
                     VLATQNTEGLKIRIVNTPEMLTMEDKGENLVLKARAKDAKTSMAVGIDLVKNGEADAF
                     VTAGNTGAGMVTALFRLGRIRGVDRPALAPIFPTATGTCVVLDIGANPDCKAENLYQF
                     GILGSIYAEKVRGVKSPKVGLVSNGEEEGKGNELVKAATPLFKASKLNYFGNVEGKEV
                     IGGKVDVAVTDGFTGNVMLKSSEAVAKLITDKIREIIKNGSILTKIGGMLVRPALGAI
                     KKLLDPSEQGAAPLLGVNGLVFIGHGRSDAIAIKNAVRVAKHAVDVKVLDAMKSAIEE
                     SLKS"
     gene            18758..18839
                     /locus_tag="PLE39_07950"
     tRNA            18758..18839
                     /locus_tag="PLE39_07950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18792..18794,aa:Leu,seq:gag)
     gene            complement(18876..20201)
                     /locus_tag="PLE39_07955"
     CDS             complement(18876..20201)
                     /locus_tag="PLE39_07955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE68298.1"
                     /translation="MDDKSGYKEPLSSQLRRRVYGNEEQIAETVRWVAYSSLFILLVL
                     VINQLFKGRYIQTLVILAGTLPIIISLLMIKSGKLSLPISIFATGTILLLTYISTVGN
                     GIHDVANMAFPIILIVTGLILRGRVLPYLTGGIILCLGWLVFGDMLGLYKADTELASL
                     PEDFLFASAIILVTSNAVHLLVENIRRSLIRAREEIAEREKAEMEREELVRQLKLKNQ
                     ELDRFAITVSHDLKTPLITITGYLGYLERDALAGNMDRVHKDISQVNEAAKNMGKLVD
                     EILDLSRIGRIMNPPREVDFAEIVGEALKASSGLLQEKQVRVKVGSEFPIVYCDFARI
                     VQVIQNLVTNAAKFMGEQPVPVIEIDTEVRNNERVFFVRDNGMGIDPKYHEQIFGLFN
                     KLNAKADGLGIGLGIVKRIIEVHEGRVWVESETGNGASFYFTLPDKPLL"
     gene            complement(20217..21866)
                     /locus_tag="PLE39_07960"
     CDS             complement(20217..21866)
                     /locus_tag="PLE39_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE68299.1"
                     /translation="MKKTKSASDEKQNAPEEKLPRRSDVLLDAIAQSAVRLLEAPDWK
                     TEINDLLKLLGEATHASHAYLFENHLDENGNLVTSQKYEWTGPGQTSELDNPDFQNVP
                     VLEEGLAAWYSVVSQGKPFYNTTKIFEEVWQDTPSRQDIKTLLDVPIFVDGKWWGVIG
                     FDDCIREMAWTKAEVHAMQVTASMLGSAIKRQKIDTALRASEEKFQTTFHHTFVPMVI
                     GRLSDKSIVDINEAFSQEIGFSREEAINHKASDLNLWADPDEHNKHHQLLQEQGFIRE
                     FKARFRRKSGEAGVALLSVAPITVNNEACVLYTLYDITKIDELLIELQNKNNELESFT
                     YTVSHDLKAPLITIGGFVGYLEKDILNGNTAKAQKDIQRVMDAVIKMQRLLNELLELS
                     RIGRFVNKPENANIGDIAREASRLLHEQVTSNKVKVIIEKDLPSMEVDRHRLVEVYQN
                     LIDNAIKFMGNRSNPEVKVGSFQQENEQIFFVKDNGIGIDPLYHERIFGLFNKLDAGT
                     DGTGIGLALVKRIIEFHGGRVWVESESGKGTTFCFTLTART"
     gene            complement(21949..22707)
                     /locus_tag="PLE39_07965"
     CDS             complement(21949..22707)
                     /locus_tag="PLE39_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HNE68300.1"
                     /translation="MPIAITRKISPRFNECEVTHIERTPIDLNVARAQHQEYVHALAA
                     LGCQVIELPEEPDLPDSVFVEDTAFILPEVAVITRPGADSRKPETESIIRALSSYRPL
                     VHVTEPGTVDGGDVLVLGKNIYIGNSTRSNADAVRQIQQLLDHYGYTVTAVDMHDCLH
                     LKTALTKVDDKTLLINPNWVDTSHFKGFDWIEVDSAEPFAANCLPVGDGIIFPTAFPK
                     TSKKLEARGYKIQAVNVAELAKAEGAVTCCSLIV"
     gene            complement(22708..23460)
                     /locus_tag="PLE39_07970"
     CDS             complement(22708..23460)
                     /locus_tag="PLE39_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNE68301.1"
                     /translation="MVKISNLDKYFGRLHVLKNVSLEVPERQVVCLIGRSGSGKSTLL
                     RCLNFLEEPSHGTIEVDGVRVEGGEKGSRHRQLVHDVRLKTGMVFQEFNLFPHMTVLE
                     NVIEGPVIVKGMDRKKAIELGEQNLDSVGMLFKKDEYPMRLSGGQKQRVAIARALTME
                     PRVMLFDEPTSALDPELIGEVLNVMKKVALEGMTMIVVTHEMGFAREVADRVIVMAEG
                     ELIEDATPDDLFKNPKDPRTKALIDRYRSGEN"
     gene            complement(23474..24292)
                     /locus_tag="PLE39_07975"
     CDS             complement(23474..24292)
                     /locus_tag="PLE39_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNE68302.1"
                     /translation="MNLFAKAESGAPKTQAELLFEAQQRKERHFRANVALTWGVLLLL
                     LVFLFSGPINVDTNFIKENMLFIAGGVGETIRISILSIILATFLALLAALGRLSTFPP
                     IYALSTFYVSLIRGTPLYLQIMFFFLALPQLDIRLPGLWAGVIALGLNYGAYMSEIFR
                     AGLASVGKGQREAAAALGMTPGQTMKRVILPQAMRFAIPPTGNEFIAMTKDSALVSAT
                     GFVHELTWRAVKVGRAQFHNLEALIMAAIFYWVMTIILSYVQGVIETRLSKGDR"
     gene            complement(24366..25268)
                     /locus_tag="PLE39_07980"
     CDS             complement(24366..25268)
                     /locus_tag="PLE39_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNE68303.1"
                     /translation="MKKLLTMASLLVVASLMLTACGGGGSTATDLLGAIKERGYILIS
                     TDPNYEPQSFLNTEGSRPSDTKCPSDALTTAEMQGFDVDVAIAVGDALGVETCFATPG
                     WDTLTAGNWADKWDMSVGSMTITTDRQKVLDFSVPYYYTPAVVAVLGDSEFQALEDLS
                     GQAICAGVSTTYETWLNGGEIGLPASSIYAKPPSGITVVPLESDQECAQALAAGRTDF
                     AAYSTSATVVNANIAAGIPVRVLGSAVYSEDLAAAFDKSSTLPTASLIAEVNKIFEAM
                     HGDGRLTELSMKWFSEDLTQAPNK"
     gene            25426..25836
                     /locus_tag="PLE39_07985"
     CDS             25426..25836
                     /locus_tag="PLE39_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNE68304.1"
                     /translation="MSVIFPSAEWLAELEAKLNSDEKYREIAKNWEGDIFFNIEPEGN
                     LKEPLTFYLDLWHGACRKVDYNPAASTYPKPTFILNSSYGNITAVLLGKMNPMTAMMT
                     MKLKVQGNMGYMMRNVPTVLDFVRCAQEVTTDIL"
     gene            25893..27704
                     /locus_tag="PLE39_07990"
     CDS             25893..27704
                     /locus_tag="PLE39_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNE68305.1"
                     /translation="MLPILKINLTTNTTEEFVIPKGWERDFLGGASLAARILYSTLTK
                     ELDPLSPESPLLFINGPLTGTNGPTTGRFVICGKSPATQLWAESHIGGFWGPELRKAG
                     YDGLWITGKAQSPIYLWIEEGRFEVRDAARVWGKDVYEAQEIVKEEVGVKAARVAVIG
                     EAGERGVYYSSICCDHGRMAGRTGLGAVMGAKNLKAVAVHGKNKIEVTAQYAQARSEA
                     NRTLKQDNEAKILHELGSSGVANYAEYLGSMPAKYYHKGSFEEVDHISGSKMAESILV
                     STSACQGCVIACGRVVNLGDGAKRKGPEYETIAGFGPNLLIGDLNDVVRLGEMCDKYG
                     MDAISASNTIGLAFHLFEMGKITLQDTGGLDLTWGSFLAARQLLRMIARREGIGDLMA
                     QGSRALGKAFDAEDEAIQVNGLETAYHDPRGVSGMALSYATSPRGACHNQSDYFFVDF
                     GHTYDEIGITYFDRHAQAEKAANVAKHQDWRTHFNAVLICIFANVPPETQVQLLNAQL
                     DLNWTIADLMRSGERGWNLKRVINNRMGLTRANDKLPKAFLEPHQEGGAAGFVPDIEG
                     MLSAYYETRGWDVRTGRPTKQKLIELGMSDIANDLWG"
     gene            complement(27728..28924)
                     /locus_tag="PLE39_07995"
     CDS             complement(27728..28924)
                     /locus_tag="PLE39_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE68306.1"
                     /translation="MIRILDIALKDLLQLMRDFKTFMFLLIMPILFTFLFGYAFGGFG
                     GNESDSRVPVGYLSLDDSWVTEELHDLLKNSEVIRLDENVFRSASDLEKLVANGELAA
                     AIIVPDGYGRAVLKDKTIRLTVLADTNTQAWMTIEAEIFTAASRVDGAVRTAGIMEQF
                     KMEGVPFNYAFDEILAAWDEPPIAIEETSSLVIKEGEENNALAHTSPGMMLQFAIAGL
                     LTAAQVIVTERKSRTLQRLLTTDTRRIQIIVGHFLAIFSLIFTQFLILICFGQFALKV
                     DYLRLPSATLLVAFTAALCISALGLLIGILARTEEQAITFSLVPMFVFSGLGGAWVPL
                     EFTGETFQAIGHISPVAWGMDGFENIVARGLGFESVVIPCLALAGYALVFFVLAVWRL
                     NATEEK"
     gene            complement(28934..30238)
                     /locus_tag="PLE39_08000"
     CDS             complement(28934..30238)
                     /locus_tag="PLE39_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE68307.1"
                     /translation="MKAFDIALKDMTRSFRSTFAVVFMFGVPLLVTGMFYFMFGNIAD
                     EGGFTLPKTNVIIANLDEGGPKFQVNPKNIPGGKEADSMGDLIVSILQSDDMADLIEV
                     SLAPNAESARSAVDRQESQVAIIIPADFSHQFADVDGKAVIEFYQDPTLTLGPAVMRS
                     ILNRFMDGMSGVKIAINFFMDEAQPEQLVLTGQVVDQYLEVSLAESDDPEAELLEVRN
                     PANGPEKEADESQNLLLGIIGPIMGGMMVFYAYYTGTSTAQSILREEEERTLPRLFTT
                     PTSQATILTGKLLSVFMTVSVQIIVLLIAANLIFGIQWGEFTSVALMAVGIIVSASSF
                     GIFVNSFLKDTKQGGVLFGGILTVTGMMGMIKIFAMNSAIANKMGDTISLLVPQGWAV
                     RGLMQAMSGESFNSIALTVLVSFLWGAAFFAIGVWRFNKRYA"
     gene            complement(30250..31197)
                     /locus_tag="PLE39_08005"
     CDS             complement(30250..31197)
                     /locus_tag="PLE39_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68308.1"
                     /translation="MNTIVPAIEVQGLRKSFGENHAVQGVSFKVDQGEIFSLLGPNGA
                     GKTTTISMLSCLLHPDEGDAQIMGHSISTNQRRVKSVLGVVPQDIALYEDLTARENLV
                     FWGKMYGLRGTALKSRVDEVLDVIGLRDRASERVGKYSGGMKRRVNIGVALLHKPKVI
                     YMDEPTVGIDPQSRRNILDSVILLKNEGMTVLYTTHYMEEAQEISDHIGIMDHGQLIA
                     HGTHDELVKMVGQQTRIDLTLNGNMDTVIPAWKELADVSRITLNGELISVLVQDSNLT
                     LPHLFETATKYSARITSVNISEPNLEAVFLHLTGRALRD"
     gene            complement(31335..31703)
                     /locus_tag="PLE39_08010"
     CDS             complement(31335..31703)
                     /locus_tag="PLE39_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68309.1"
                     /translation="MNWKMYYDEAEKIIFIKTNGVLEIKSATLMRNEGVELIRKHNCM
                     RCLLDHRDISEDALATMDIYELPKRYAELGIPRQFKIALIVPKKLETNLKFYETVCRN
                     NGFFASLFVDYESALDWLKK"
     gene            31895..>32797
                     /locus_tag="PLE39_08015"
     CDS             31895..>32797
                     /locus_tag="PLE39_08015"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNE68310.1"
                     /translation="MKLVTVAQMRAIEKEADANGVSYAQLMQNAGRGLAEVVHALGQE
                     NGWEEVTALVGAGNNGGDALVALTWLAQAGWTTHAYLVRRDSANDDLVTAYLAAGGEV
                     ADFEDDRALAVFLNESDVLLDGLLGTGIRLPLKKEAALVLSNAKEMISEMEIPPFVVA
                     VDCPSGVDCDSGEIAEETLPADLTLTMAAAKYGLLKLPAFEFIGELQVVDIGLPDGLS
                     SWQEVNTEAADDELVAVLLPERTPASHKGTFGTAFVVAGSTSYSGAALLAATAAYRIG
                     AGLVTLAVPEPLHVALAGRLLEATW"
BASE COUNT         7938 a         7762 c         8567 g         8530 t
ORIGIN      
        1 gctgtgttgg ataccgcacg gaataaaatt cttaagtaag catgctcttg ttgaggataa
       61 ttttattaag ccggttttcc ctttgaatcc gagacctatg gcatgttcgc tcatgaatcc
      121 atgccgaagc tgacaattgg caaaaaggtg tacgtcaaat tcgacccgaa caaacctgaa
      181 atttcggctc tgatgcgggc ggtagatccg taacttgctt gagtgatatg ggacaattcg
      241 ccccgaaata ccgtggcatg gaactgcctc agtttgagat aatctgttat aaaatacggg
      301 taaaattaca tatcattttc atcaggagtt gtaactgtgg attttagctg gatttcaaac
      361 cccgaatcgt ggattgcgtt ggtaacactt atcgccttgg agttggtact tggcgtggac
      421 aatgtgatct ttatttccat cctttcaggc aaattgcccc aggaagacca gcagcgggcc
      481 agaacaaccg gtatttcgct tgcggttatt accagagttt tgttattgct ctctctatcg
      541 tggattatag gtctgacaga gccgcttttc catcttccat tttttgaagg cgaagttgcc
      601 gaaatcaccg ggcgtgacct gattctcttg ttgggtggtc ttttcctcct ttggaagagc
      661 acccacgaaa ttcatgataa attggaaggg aaagagggat ccgcttctgc aaaggtggcg
      721 gcgaattttg tcagtgtgat cattcagatc atgcttttag atattgtctt ctcactcgat
      781 tcggttatta cggctgttgg tattgcagac aacattatta ttatgattgt tgcagttgtt
      841 gccgctgcag ttgttatgat attcactgcc aagcccattg gggattttgt agaaagacat
      901 cctaccatca agatactggc actaagcttc cttcttatga tcggttttac cttgattgct
      961 gaaggatttg gtgttgaaat tcccaaggga tatgtctact ttgcaatggg cttttctgta
     1021 atggtggaga tgctcaacct gcgtgtacgg gactctcatg tcaagccggt caacctgcgt
     1081 gatccctata aagaggaaaa ggttgaattg cagcccgctt ttgttgctgc gtcaaaagct
     1141 gcaacagtaa caacagaaaa actaacaacc tcgcccgcta aaaaggcttc taccaaaaca
     1201 aaggcaaagc cgaaatcaaa aactaaaaag agctgagaac aaaaagtgga cttctaaaga
     1261 agtccacttt ttgtttgatc tatacccgat gggcaccctg ttttccaggt ttcgttatga
     1321 cagatttggt ttcaatgcct cgctctacaa aaacactctc aatttctgat ctggccaatt
     1381 ccgcatgatc aggcggaaca aaggtgatga tcgagggccc tgcaccggag agtgccgctg
     1441 caccaaactg tctggctttt ttgtatgctg ctgtcccccc ggaaatatgc cttaaccgat
     1501 agggttgatg gatacggtca tccattactt tttgaagaag tgcaaggttt cctgttcgta
     1561 atgcttcaac tacaagcgat gtgcgcccga tgttgtacac agcatcggca cgtgaaacgg
     1621 attttggcag gaccgcccgc gcggtttttg tcagccattc cacgatgggt ttgacaatga
     1681 caatcgttaa ttcaggcact tcatatctgt gcgaaatgat ttcttctcct gccattactg
     1741 aaacgaccaa tccgccaaac aaggcagggg caacattgtc ggggtggcct tccatatcag
     1801 atgcaaactt gagaagttcg ttctggctga gtggagaccc cagcatttca ttcgcaccaa
     1861 atagccccga aacgatggct gctgcactcg aaccaagccc gctgctcatt aagatttcgt
     1921 tgtgagcgca aatgcgtacg ggactcactt ttttgccgca aaattcataa agattcgaat
     1981 acgatttggt caagaggttt tttgtgcctt tattaagttt ttcggcaccc tcaccagtga
     2041 cgtgataagt caacttttcg gcaggctcaa atgtgatttc gttccaaaga tctaaagcaa
     2101 ggcccagaca atcaaagcct ggacccagat tggcggaggt ggcggggact cggattttta
     2161 gcatggcgac attatataat acctgccgcc aaaaattgct tttttcgtaa atttcagtgt
     2221 cgataggtat aatcgtgtca tcacttaacc tggagttgct atgacctatc aaggcttgat
     2281 ccatcgctat gggcagtatc ttgacttgcc cgcccacaca aagacaattt ccctgctcga
     2341 gggaaatacg cccctgattc ccatgccacg tttgggcagg gaacttggct gtgaattatt
     2401 catcaaattc gaaggcttga atcccacagg ttccttcaaa gatcgtggga tgaccgctgc
     2461 catcagcgag gccgtaggcc gtggtgcaca gactgtgatt tgtgcttcta caggcaatac
     2521 agcggcgtct gcggctgcat atgctgctcg ggctgggctc cgttccatcg tcctgattcc
     2581 tgaaggcaag gtggctgttg ggaagttggc tggagccatt gcctatgggg cagaggtcat
     2641 ccaaattcag ggttcctttg atgatgccct aacccttgtg gtggaaatta cacaaaagac
     2701 gcccattgct ttggtcaatt cgatcaaccc atttcgcatt gaagggcaga aaactgcgtc
     2761 ttttgaaatt tgtgacgatc ttggttcggc gccggattgg ctatgcctgc ctgttggcaa
     2821 tgcggggaat atcacctcgt attgggcggg tttcaaacaa tataaaaaag ggttgcccca
     2881 agtgctggga gtgcaggcag caggggccgc tccacttgtg ttaggtcatc ccattgaaaa
     2941 accagagaca atcgctaccg ccattcgcat aggcaagccc gcgcgcggcg agcaagcttt
     3001 acaggcggcg gaagaatcca atggaaggat cattgctgtt tcagacgaac agattctttc
     3061 cacacagaag atattggccg cggaaggggt ttgggttgag cctgcatctg ctgcggggct
     3121 ggctggtttg ttggcagagt cagcttccgg taagttcgaa gcgaaaggca aacgaatcgt
     3181 agtggtttgc acaggccatg ggatgaagga tccatccatc atcaccgaat ctttcaaatc
     3241 ccccaaagtg atctcggcag attacgatgc gttgatcagt gctgtgggtg cgtagtactg
     3301 gtgtaccatt cgtatggcgg cgggccttta tataataagg cggatttacc gatcaatcca
     3361 aatttgctaa aaggagccaa acatgttttt taatgagtgg aatattcttg ttgttgatga
     3421 tgaacccgac gtccttgctg tgaccaaact ggctttgcgc gatgtgaagg tatatggttt
     3481 gcctgtaaag attcatacgg catccagcaa ggccgaagcg attgaaatgc tcaagggtcc
     3541 gctggcttta cagggctcta cagagccgat tgtggctgtg gctttggtgg atgtggtaat
     3601 ggaaaacgat caggctggtc tcgaactgtg caaattcatc cgtgaagagg cgaatggaca
     3661 ttcagtgcag ttgttcatcc gtactgggca gcctggtgtt gctcctgaac gttcggttat
     3721 tgataactat gatatttcag gttacttcac caaagttgaa ttgagcgagc agaagttgta
     3781 cacgttggtg aagagcggtg tgcgtcagtg gtttaccagt tggtatgcgt tgatgactga
     3841 ggaatcgaca aataacattg ttacgcactc tggctctcgc aaagactttt tggagaatgg
     3901 cattggttgg tttggtgagc ctggtccgca ggaaggcgag ggtgtcactg gttttatttt
     3961 tgatagaaac cttattgtga gtgactatcc cacccagatc aagccgttgt atgatcgttt
     4021 gaatgcattg aagcccgtca ttgaaacggc agagggccac aagctcgcca tggatgaaat
     4081 gggcaatctt ctggtcaaga cggtcaagac caagacgaca gcagattatg cctatgtagg
     4141 tgagtcatcc atggtcatgc cgcgattgct gcttgaattg accttcaaga gtggtttggt
     4201 cttttccact ctatggaaga agatcaccga tgacgaaaag agagccgctg ctaaaaagcc
     4261 tgttgcgaaa aaatccgcta aaaaacctgc caagcctgcc aaaaaggctg taaagaaggt
     4321 cgcaaaaaag gttgcggtca agcctgctaa aaagacgacg aagaaagccg cgaagaagaa
     4381 gaaatagtgg ttttcttgtt gaaacgttct tgaaaatgta acccaaggcg gatgctgtgc
     4441 tgagtaattg ataagccatg catccgcttg tgttttgaag gtccatgcaa aatgacgaaa
     4501 aagaatcctg tgctcaatcc aaatattctg caaaagtttt cgttctttgc ttctttttcc
     4561 aaagaccaat tggaagagat cgcaaagtcg gcgccaaagg tggcctgtaa aactggcgag
     4621 atcgtatttc ggcagggtga ccgctctgcg acgatgtatt tgattctcaa aggtgcagtg
     4681 aaaattgaga gagaagacag cgtgggtggg atcgttagtg taggccagtt atctgtgcat
     4741 caggtatttg gcgagctggc gatgttgagc aaggagcctc gtcaggcgac tgttaccgtg
     4801 acgaaaaacg ccgaattcct tgtgattgac cgtaaaatga tgttggagat cattgccaaa
     4861 tcagaccccg aggagatcat tgaagttttt tctgtattga gtgagcaaac tcgtgctgca
     4921 aacgaccgcg aatttcacga gaccctctcg cggcgtaccc tcgaagcaca gatggaagtg
     4981 gaaaaacaac gcgctctgac gcagctggtg gctggtgtgg cgcacgagat caatacaccc
     5041 cttggaataa ttaacacagc ggtcagtatt atggctcgtg aattatcaga gcccagcgaa
     5101 atcactgccc agcgtgcggc agatattgcc gaatcgctcg aattgatgcg ccgcaacgtg
     5161 gagcgggctc atcgtttggt acaggatttc aaaaaggtct ccgccagtca gttaatggac
     5221 gagattgaaa cctttgacat ttctgaaacc attcgcgaaa ctgttgactt gatgatggtt
     5281 ggcttgaaga gtagtcaaat tcaggctaaa ttccacgata aattgacact cacgcaaaaa
     5341 gcatggatgg ggtatcgagg cgggctatct cagattctga tcaatctgtt gaccaatgcc
     5401 gaacgacatg cctatcccaa tggagcgggc ggattgatcg acattaccat cgaaatgttc
     5461 gacgaaaaga attatcgcct gaccgttcaa gattatgggc agggtattcc agctgaaaac
     5521 caacctcgta ttttcgaacc ctttttcacc acagcacgtg ctcgtggcgg cactgggttg
     5581 ggattggcca ttatccataa ccttgttgtc aacgttttga agggtgaaat tcaattaagg
     5641 tcagaaatcc agaaaggcac agcctttttg gtggttttcc caagggattt gcccaaataa
     5701 actgttttca gcatgttcaa gggattcaat ctcctacttg acattcccat ttaatgtctt
     5761 gataatagga tggatgcaat tgaattcatt aaaaaccgag agtgctgaaa cctgtccatg
     5821 aacgaaattc cattagttgt tgaaaactta acgtttaagt accgtgttcg ccctgagctt
     5881 gcgatcgaaa acatttcgtt cgagctaaaa cagggtgagt tgcttttaat tgcaggttca
     5941 agcggctgcg gcaaaaccac actggcccgc tgtattaatg ggcttgttcc gcgcagctac
     6001 cgtggcgagc gcagtggacg agtccttttg catggcaagg aagtgaccga actaaagatc
     6061 gccgaaatgg cgcaggtggt tggcaccctg ttgcaagacc ctgaacgtca gatcgtagcc
     6121 agtaatgttt ataacgagat cgcttttggc cccgaaaacc ttggcctgcc gcatgatgag
     6181 attgaagcac gcattgaaca ggccctgaag cgtttgaaga tcgaatattt gcgcgaccgt
     6241 gagaccttca atctttctgg gggcgaaaag caaaaagtgg cactggctgg tttgcttgca
     6301 atgaaccctt ccatacttct gctggatgaa ccccttgctt ccctggaccc cgcctcagca
     6361 cacgatgccc tcaagatatt cagaagcctc gcagatgagg gaaaaacaat cattctggtg
     6421 gaacatcgtg tggaagattc catcgaagcg aaacccgacc gcctgcttta catggagagc
     6481 ggtcgtatca agtatttggg tggcattacc agcctgccaa aagaaattga ccatacccag
     6541 gtaaaactcc ccgctcaatg ggtggtgcaa cgggtcaagg aagataaaaa acatgccgat
     6601 gttccgcccg ttcaaaaagg ggaaagtggt gatgagccgc tggttgtttt tgaagatgtc
     6661 agttttcgat acgacgatga atacccgctc atcttgaaag atgtcaacct caccatcaat
     6721 cgcggtgacc tgatcgctgt ccttgggcca aacggcgctg gaaaatccac gttggtgaag
     6781 catgccattg ggttgttaaa acccacctca ggccgtgtgt tggtgcaggg tgaagatacc
     6841 cgaaaaatga gcgtcgccca aattgcgcgc gtgttggggt ttgtctttca aagtcccact
     6901 catatgttgt acgctcccac ggttcaagag gagttggaat tcgggcctaa gaatctcgct
     6961 tttgacaaag aggcaatgga caaatcggta actgaaagcc tcaacgcggt caacttgaaa
     7021 ggttttgaag aatacccgcc tttggggctg tcctttggtc agcaaaagcg cacaactatt
     7081 gccgccgtgc ttgccatgca atcgaagatc atggtaatgg atgagcctac tgccggacag
     7141 gattttgcca attacacgcg ttttatggat gcattgtgca gcgcctccaa taacgcaaaa
     7201 tcgatcctct ctgccaattt tgactcaacc ctcttcatta cacatgatgt tgatctggca
     7261 gtgacctacg caaaccgtgt gctgcttttt ggagataaac atgttgttgc agatggaaaa
     7321 ccagaggagg tcttgaaaga tattgacctg ttgatgaagt atcgtgtgcg ccccacttcg
     7381 cttcttcacc tgaacctgga cctgtttgca aaaacgaaga agtttttacc cgctgaagca
     7441 ttggccgggt ttgtgtcatg atcttccctg aaacgcattg acggattcag ggtttatcta
     7501 ggtatagggt cagctcgtat cgaataccaa tcaaaaggag agaatcatga acgacaaaag
     7561 tacttgggaa tttggaaccc gccaggttgt atatggtgca atcggcgcgg cattgtatgg
     7621 tgtgctgtca ctggccacca attacttccc ccttccggcg gctggtaatg taaccttccg
     7681 cccggcggtc gccgttttga tcttttttgg cgcggcgtat ggcccctggg ttgggttgct
     7741 tgcggggttc atcggcaata cccttggtga tgctgccatg gggtggggct tctattggaa
     7801 ctggagcctc ggaaatggtc tgatgggttt ggtcgctggc ttggcgatgc caatgctcaa
     7861 agatttcaag gctcgtggcg atattatcaa ggccattatc tacggtttga tcggtattgc
     7921 cgtgggtatg ttgtttgcct cagtcactga aaaattcatc ggtggccttg attggaatac
     7981 agccctggtt ggctacttca caccggcctt tattgggaat gccgtagtcg tggtgatcct
     8041 gctgcctgtg ttgatgatcg ctttcgccgc agtcgcttct cgtagaggcc gctaaaacat
     8101 atgttggtca cctggaagta ccgtccccgc gatacattta ttcagcgatt ggatccgcgc
     8161 acaagattta tttacatggt gtgcattatt ttcgcgctga ccctgcccga tatctgggac
     8221 tatcgcatca tctttcctct gttcattagc tcgctgaccc tgtatttgct tgctcgaatc
     8281 gagtggaagg atgtccgacg tgcctggata ttcatcggtg tcttcgtttt ctttatcgtc
     8341 ggcttgaacg ccctgctatc cggccgaggc ggtcctgaca cggtgaccaa cgtttattct
     8401 ccaaccatat tcaacctgcc tctgaaaatt ccctttacca atatcggctg ggatatccca
     8461 gttacagttg caaaattatg gtttgccgtc aaccagatta cgcgtatgtt gaccatggcc
     8521 attctggcgt ttcccattcc ctacacggtc agccccacta tttatgggac cacttttaag
     8581 aggatgggta cctcggataa gttttccttc acgatggacc tggcttttcg ttttctgccg
     8641 acctttgccc gtgatttgat gatcacaatg gatgcccaac gggcgcgtgg atacgaatta
     8701 gacaagttga agggcggcat agcggcacgc cttcgaaaac ttgcaccatt gatcattccc
     8761 gtggtgatgc aatctacggt gacaggggaa gaggtgattg acgccatgga tttgcgggct
     8821 tttggtacgg ctccccgcac atggctgcag agtcaagact tgcaatacaa acccatggat
     8881 tatgtgatgc tcagtttggg tttggcgatt cttgtagtgt gttgtatttt gaaatggggg
     8941 tttggcatag gcggtttctt tgtgccagat tttttcatga acctttttgt gtaaaacaaa
     9001 aacgcatcgc caaaagcggt gcgttttttt ataagacttt ttgacacatt cctagttttc
     9061 gcggcgtgga taggtttctc cggccaatac tttgaaatcg agatggtttt gctcaggggc
     9121 gaaaacacag gtggcaaaat ctgtaaatgc gcagggcgga gagtaggctc ggttgaaatc
     9181 aatgaaaatt tttccgtcgg cttcgatatc tgcgatcaag tagcggcctg tgggataggt
     9241 ctcatctttg ctggtggggt cccagaagcg gacaaaaagc gtgccgtcat cttccttgtt
     9301 gatatccagt tggtagtgat ttccaccgaa ttcgaaagat aaataacctt ccaccgggaa
     9361 ttcggatttt tcgcctgtta tatctggaaa gtaagccatc ttggggcgat catacggcgt
     9421 aaatgtggca gggatacgat attgctcatc gatgtcgtac cagcttcggg cggggaaaga
     9481 tcgtctctct tcgcgttggt tatcccacat gcgtacgccc agtctgttgc cgcgttgaat
     9541 caccacgagg cagatatcct caaatttaat aaagcttggg ttttcagaaa tatcggggtg
     9601 aagaatggca aaatctgaaa gtttttcgtt tacatgcact ttatgaccat cggtggtgcg
     9661 catgctcact gattttccgt tgtattcaag ataccctaca acaggtgaaa tacgggtggg
     9721 caattgaatt tcgcacttgg ggtcggcgcc aaactggttt ttgccgagtt tgagccagta
     9781 gagcccggca agcgagagcc atccattttc tctgcggatg gtttcatcac gttccttgcg
     9841 ccattcattg agtagttgcc gataagtttt tgtggtcatg tttgggatta taccaacaaa
     9901 aaaaggcggg ccgttgccga cccgcctttc tgatttgtta tgctgttcta cccaactgtg
     9961 gggaccatgg tgaggtagat aaagaggcca gcaatgaatg caagcaggcc gaacagtgag
    10021 aagagggtgc gcatggtgct ttctgcgccg agcagggacg cccaagccgc gagtgcaaga
    10081 ccaagggcca tgatcaacat gacgagttgt agttgatcgc cgcgctcatc ccattgtgac
    10141 gcaaggtcaa agttggtgtc ggattcatcc cagagaacgg ttgcttcggt gtacatatct
    10201 gtgtaatatt gctcatccca gacggtttca gggtcacgtt cgatggctgc ggcaagttgc
    10261 tctgagaaac tggtttggta gtattctgcg atttcggggc gttctccggc gttgatatac
    10321 cagttgtcga agtagttgta gtcctgggtg atgtcctggt tggcgcgcaa gtactcggcg
    10381 ttgccgtcat tcagggtttg catgccaagg atctcatact tgttctgctc tgaatcagac
    10441 atggcgccct gatagctggc gatggcggta aatgcgctta gtaaagcaat catcgtaccg
    10501 aggaagattt cattgctcaa aaaaccaaaa gcctttttca tttctctcac tccttatgaa
    10561 ttttggataa attacttggt ggaactacgg cagagattat aaaacatgat tttgcattgc
    10621 atatttacaa aattgttata aaatcatgcc agtttgcaag aaagtatatt catgcctttg
    10681 tcaaaagact gacagggccc caaggagaat cggtatgggt ttgaaaccag ataagtggat
    10741 tcgcaagatg gcgcttgaac agaaaatgat tgaacccttt gtagatagcc aggtgcgtga
    10801 aggggtgatt tcttacggtg tatcctcgta tgggtacgat gttcgagtgg cgaacgagtt
    10861 caagattttt acgaacgttt tttcagccac ggtcgatccg aagcaatttg acacaaaatc
    10921 aatggtggat tttgtgggtg acgtttgtat cgtgccgcca aattcttttg cgctggcgcg
    10981 cacggttgag tatttccgca ttccgcgcaa agtgctgact gtgtgtctgg gcaagtccac
    11041 atatgcccgt tgtggcatta ttgtcaatgt gacaccgttt gagccggagt gggaagggta
    11101 tgtgacgctg gaaatttcga ataccacacc gttgcctgcc aagatttacg caaacgaagg
    11161 gttggcgcag gtcttgttct tcgaagctga tgaagaatgt gacgtttcct acgccgataa
    11221 aaaaggaaag taccagaaac agcaatcgat tgtgttaccc aaattatagt attgtgataa
    11281 tctgatactg attgaaattg gttttatcaa ggagagcggt tatggctgat tccaatgaag
    11341 cacagacaat gcaaaagcaa atggatgaca tgaacaagat gttgttgcgt gtcatctcgc
    11401 atctggcacg gtccacagat ggtcagactt cgttgattct gagggacctt acacagattt
    11461 ccagcaccat ggccgcactc aagcaaagta tcactggcta tttgagcacg tacgaacgtc
    11521 aggtgggggc attggtgggc gtaggaagcg caattaattc ttcattgggg ctgaagcgag
    11581 tgctggaaat ggtgatggat tcgctgatcg ccttgatggg agcagagcgc ggtttgttga
    11641 tgctgcgcga gccgaatggc gaattgaagg ttcgtgtagc ccgtggtggt gaccgtgaag
    11701 acctggcaca ggaagttttc tcgatcagca atacagttgt gcagcaagtg gctgaaaccg
    11761 ggaatgcaat tttgacaact aatgcgcagg atgatccgcg tttcggcagg caggtcagtg
    11821 tggcggcgta tcgtttgcgg tcaattttat gtgcgccgct caagatcaaa gataacttga
    11881 tcggtgtgat ctatgtagat aaccgtgctc ataccggtat ttttggcaac aacgatctct
    11941 ctttgatttc agcgttttcc aatcaggctg ctgtggccat tgataatgcc agcctcttcg
    12001 atgatctaca ggaaagcaat gctgaattgg agatcgcgta tcaggccaca ctggaaggtt
    12061 gggtgcgcgc actggacttg cgtgacaagg agacagaagg ccatacgcaa agggtgactg
    12121 cgctcacaca gcgcctcgct caagccatgg gtgtggatga tgatgccttg ctccacatca
    12181 cacgtggagc gcttttgcac gatatcggta aaatggcaat cccagatggc attctgctca
    12241 aacctggcag tcttaccgat gaggaacggg cgcatattca gaaacaccct gtatatgcct
    12301 atgagatgtt aagcccgatc aagttcctac accctgcatt ggacattccc tactgtcatc
    12361 atgaaaaatg ggatggtaca gggtacccgc gcaaactaaa aggagaggag attccatttg
    12421 cagcgcggat cttctcggtt gtggatgtgt gggatgcgct tatttcggat cgcccatacc
    12481 gcaaggggtt gccgccaagt gagattcgtg aacgcattca aaaagattcg ggttctcatt
    12541 ttgaccccaa ggtggtagag actttcctaa gccttgataa tcttgctgtc tgaatttctc
    12601 ttgtgaatcc agcaaccata aagcacaacg acatggaatt agtttttctc catgtcgttg
    12661 tgcttttaag atcttttatt gtagtttgct gttgtgaact gttacgtccc gagccattga
    12721 cctgccatct tggccaaccc tgcggcagct ccctgtcttg cagtgcattg cggcagggat
    12781 tcgaggaaca aacgcccgta ttgttttgtc agtacacggc ggtctaaaat ggcgacgatg
    12841 ccgcgatcag aggcagagcg gatgaggcgg ccgaatccct ggcggaattt cagaatggat
    12901 tcgggcaggt agtattcgtt gaacgaatct tcataaagtt ctgagcgcgc agcaatgatg
    12961 ggatcagagg ggacgtcaaa tgggagtttt gtgatgaaaa cgaccgaaag cgcatcaccg
    13021 ggcacatcca caccttccca gaatgagcgc gtgccgagca acactgcacg gtcggtggat
    13081 ttgaacgatt ccagcagggc attgggtgaa gcgccttcgc cttgctcaaa aacaaaaata
    13141 tcctcgcgtg ccaatggacc ggtgatggcc tgtgctgttt tcttcagcgc ggcataggat
    13201 gtgaacaata cgagcatccg cccgcctgtg gctttggctg tggcgatgat ggttttatcg
    13261 agcatttgct gatagccatg gatatttggt tcagggatgt cgtttgccac gtaaagcagt
    13321 gtgctggatt cgtaatcgaa gggcgagccg agcgcaaccg tatccacttc ttcagcggag
    13381 agggtgttgc ggatgtattg aaactctcca tgtgtggtga gtgtggcgga agccatgatg
    13441 acactggctt tttcatgcca caggtgtttt tgcatcagtg gaccaacccg cagtggagcc
    13501 gcgttgagcg aaagcctttc gccgcgcgga ttgacctcca cccagtagat cagttcattg
    13561 gatggtttgt gcatcatgcc tgaggatgcg gcttccgcct cggtcatccg acgcatgaga
    13621 ctgctcaagc ttcccatgat atcttccacg ctgtcatggc cttccgacat cagatcgccg
    13681 aggcctttgt acagttcatc gagggttttc aataacagtg acatggtttc gcccacctgc
    13741 ccccagccca tctctacatc gtcccaaccg ggcagggtgc gtgtggcagg catgacgcgc
    13801 atctgccatg aataattgct ttgcggctgt ccttcacgct gaatggcaat gaaatcgctc
    13861 aggttcatga aaaactgttt agagagttgt tcgacgcgga atgcctggtc ggtggcgcgt
    13921 ttaatttttt gttgaagcag gccaaagtcg gaggggcgaa gcgaggcatg cagatcggtc
    13981 aaaatacgac cgagaattcc tgccgaagat ccgcctaatt ccttcatcat gcgttcgagg
    14041 tcgttttgag tcattcggaa agacagcgca cctgttactg ccgattccat gtggtgcgct
    14101 tcgtctatga tgacgtaatc atattcgggc aatactttgc ttccagatgc cacgtctgac
    14161 agtagaagcg catggttgac gatcaagata tgagaattga gcgcggcttg tttggcacgc
    14221 tggaatggac aggaaccccc catgcgcccc atgcaggttt cggtggtgca gttatcgtcc
    14281 tctgcagaaa gccgtgccca tgcttcgcgt tcagtggggc ccgtgaggtt gagttcgttt
    14341 cggtcgccgc tggcgttatc gagcatccac accatgattt tggcgagcac ccgcatttca
    14401 ttggcatcct tcggcccgtg cgagcgcatg tattcgagct tgcgcggaca gagataattg
    14461 ccgcggccct tcatcaccgt ggcgttcagg ttctcaaggt tgagcgcggc tttcaagttg
    14521 gggatatctt ttttgatcaa ctgatcttgc aggttgatgg tgttggtgga gacaaccacg
    14581 cgggtgctgt tttgaaccgc gaaataagcc gcaggtacaa gatacgcgaa ggatttaccc
    14641 acaccggttc ccgcctcgac catgagatgg ttgcccgtgg aaagggcatt ggtcacagct
    14701 ttgagcattt ccacctgttc aggacgatgc tcgtatgatt cgaagtattg cgagaagggc
    14761 ccgccatatt caaggacggc agcgatctct tcagggtcaa gcggaagcgg ctcttcggtc
    14821 ttttgaatgg aggctgattc tcgttttgct gaatcaaaca tcggtccaga aaaagaggcg
    14881 agcctggctc gtttggcttt gggtgcttcc ttggcgcgtt gtttcaatgc ctgttggaag
    14941 acccagcccg catcccaatc cacaaaatcg ccgagctgca cgatctcatt caaggtttcg
    15001 agcggaagtt cactagccat ttcaaacaaa cggacataag ccgcctgtgt gacgcgggca
    15061 tcatccaatg cgcggtgagt ggcagggagc aagatattga gttgctggcc cagcgagccg
    15121 aggttgtaac ggctggcggt gggcattaac acagaggcaa gttcgtaggt atctatggtt
    15181 tgctgatatt cgaataaccc cgccttgcga agaaaaccga tatcgaattt cacattgtga
    15241 cccaaaatgg gtgcgtcacc aacaaaggcg agtaggtcgt tggcgatgtc gcgtaaacgc
    15301 ggtgcctgcc gtaccatggc atcgtcaatg cctgtcagcc cggtaatgaa atcaggaatg
    15361 tgcctgccgg ggttgatcag cgtgttgaat tcactttcaa tgcggcggcc attgaacttt
    15421 acggcggcaa tttcaataat ggcctcgcgg ttttcgtcga ggccagtggt ttcgatatcc
    15481 agagagacaa ttgaggtcat attagaacaa gtgtaccatg aaaagtttgt ttcgcttgcc
    15541 gtgcgggttt atgagagtgg ctgacaagaa tagtttgtga cttcaccatc tttttgccgc
    15601 tcccgcattc atcccttttc agatttcaaa gggcggttgg cttgatcgaa gaggatggct
    15661 aaaaatatcc acagcattaa tcggaagatt tccactgcca gcatggtatg gcgtgtgacg
    15721 ccaatggagt cggcaaaaaa attaataacc atcattgccg atgcgctggc gatcagccaa
    15781 agactcaacc aggcccaggc gccgaattcc ccatttcttc gggcgatcgc tgaaacgacc
    15841 aatcccatca ataacagcag atcgaggata aaaacgagat gggtgtttgg atgcagaata
    15901 tcgttgagcg cattcaaaac ctttcttgtg cctgtttgct ccgagaagaa atagggctgt
    15961 gtgttttcag agaaaatgcc ccccaaattc gaggtgaatg aggtaagtgt gtagccaggg
    16021 tgagcaagca ggaagcgggc ataggcccgt ggtgcgtttt ctctgaacca ggttgggtat
    16081 tcagcagatt tggggtctgg catgccgatg cccgtcataa actcagcgcg ggctgggtat
    16141 ggcagaatga actctgcaaa cacattggtg agcggttttt tccagcgcct gctttccatg
    16201 gcgctttgca accctacaac tgtcagaaca agaatgaccg cactgatgac gagtacgctt
    16261 ttgtttttgc gcaggcgcgg aaaaatcacc agtgacacgg ccaaaaacgc gaaaagcaca
    16321 gcaagggcat acacgttcga atctcttgta aaagtccaca gggccagtga aaccagggtc
    16381 aggatggtta gcagaattga ggtcggcctt tgatcttctt caatggcagt gaagcaaatt
    16441 ttcagcaaca aggccaggct gatggcaaac agcgaaaagg tcaatgactc agagccgagg
    16501 atgctgtccc agtctgcgat ggcaggcgta aaaccaaatg cggtgatgga gattgccgcg
    16561 aacagctttt caaatccgcc tgtcagtttg ctggctacgg tcaatgccag caggctccag
    16621 gcaaggatgg aaagcaggtt ctgcaacagg gcaatccttg taaagcacgg ctgtaccgcc
    16681 cgctcggttt caagcccaag ggcggggtgg ctgatggcgg ttaattcgca ctcctgaacc
    16741 tttgccagat gataaacaag gtttgtggtg aaaagacggc ttctggtgag catatccctt
    16801 gaaaagaggg gaggtgcggc ctcgtcgata taactgggcg tatccgcatt ggccacagaa
    16861 agattgatac ttccgtacgc cttcagccga aagaaagcca tgcctgccag aatgacccag
    16921 atgagaaggg tcaccccgat tttttcagat accttcatgg aatcctctgt agcaggatgt
    16981 gactaggatt ccagcgcgtc actcagcgcc ttgatctgct cgttggaaag tgaccgattc
    17041 ttgccgcctt gacgcatgaa ttccttaagc tgtttcaaac gcatggccgc gatgggagaa
    17101 tcgcccgcat ccacttcgat ctcatcattg gtgaagacca aaatgggttt gatctcaggg
    17161 atggtggatt cgtccatttt ttttgcgaga aattttctca acgcctgcac ttcattttca
    17221 gcatctattt cagggcgacc caaaccctcc tgcccaaaga cgctcatgta ggcctgtaga
    17281 aaccctccat tgctcagttt ccagcgattc ttttggtaag agatcttccc ccgctggtgc
    17341 agcggatgca acacccatgc ccctgaaggc ccaatcagca ggtgtgaaac aggcgatgaa
    17401 aaatgataga tcacaaaatc gctgtgcaag ccttttagcc ctgcgtcaaa tttttcatcc
    17461 gcgcgcggcg acctgcccca gcggttgccc atgtagatgc tgatctgggt catcgtaaac
    17521 ccgatcacaa ggcagacgat cgaatatata aaatattctg gtacggtgaa agaaatgtac
    17581 aagcccagcc caagcactat tagagaggca aggctcatcc attggccgat ttttccattg
    17641 cgttcgatca atttctcgtt tttgatgatt ttcatttagc ttttcaaact ctcttctatg
    17701 gcagatttca ttgcgtccag cacttttaca tccacggcat gtttagccac gcgaacagca
    17761 ttcttaatgg cgatggcatc cgaacggcca tgaccgatga acaccagccc gttgacgccc
    17821 aataaaggcg ccgctccctg ctcactggga tccaacagtt tcttgatggc tcccaaagca
    17881 ggccgaacaa gcattcctcc gatcttcgtc aaaatgcttc cgttcttgat gatctctcgg
    17941 attttatcgg tgatcaactt ggcgacagcc tccgaggatt tcaacatcac gttccctgta
    18001 aaaccgtcag tcacggccac gtccactttg ccgccgatca cttccttgcc ttccacattt
    18061 ccaaaataat ttaattttga agccttgaac aaaggcgtgg ctgcctttac caattcattg
    18121 cccttgcctt cttcttcgcc atttgaaacc aggcccacct tcgggctttt tacaccacgt
    18181 accttctcag cgtaaatact tccaaggatt ccaaattgat agagattctc agctttgcaa
    18241 tcggggttcg cgccgatatc aagcacaaca cacgtgcctg ttgctgttgg aaagatgggg
    18301 gccagggcag ggcggtctac accacggatg cgccccaagc gaaatagcgc ggtcaccata
    18361 cccgctccag tgttgcctgc agttacaaat gcgtcggcct cgccattctt cacgaggtca
    18421 atgcccactg ccatcgaagt cttcgcgtcc tttgcgcgtg ccttcaatac caaattttca
    18481 cctttgtctt ccatcgtcaa catttcgggt gtgttgacga tgcgtatctt caacccttcc
    18541 gtattttgtg tggctaatac aggtttgatc ctggcttcat cgcccactag aatgatctcc
    18601 gctccatatt cacgtgcggc catcaccgcc ccttccacgt ctggaacagg gtaactgtca
    18661 cttcccattg cgtctacaac gattcgagcc atagaaatct cctgtgataa ttatgcttga
    18721 caagaaaagc aagccgatta taatacgccc tgcttacgcg ctggtgctgg aactggcaga
    18781 caggcatggt tgagggccat gtgtcgcaag acgtggaggt tcaagtcctc tccagcgcac
    18841 acctcgtccc cattatcaaa atggggacgt tttgtttaca aaaggggctt atctggcagg
    18901 gtaaagtaaa aactcgctcc atttccagtt tccgactcaa cccaaaccct gccctcatga
    18961 acctcgataa tgcgtttgac gatccccagc ccaattccca acccatcggc ttttgcattt
    19021 aatttgttga acaacccaaa gatctgttca tgatattttg ggtcaatccc catcccgtta
    19081 tcccgcacaa aaaagacacg ctcgttattc ctcacctccg tgtcaatctc gatgactggc
    19141 acaggttgtt cccccatgaa tttggcggcg tttgtcacca gattttggat gacttgaacg
    19201 attcgtgcaa aatcacaata cacgatgggg aattcgcttc ccaccttaac cctgacctgt
    19261 ttttcctgta ataggccact cgatgccttg agcgcttctc ctacgatctc ggcaaagtcc
    19321 acctcccgcg gcggattcat gatgcgccca attcgcgata agtccagaat ttcatccacc
    19381 agtttcccca tgtttttcgc cgcttcattc acctgactga tgtctttatg aaccctgtcc
    19441 atattccctg ccaaggcatc cctctccaga tatcccaaat atcctgtgat cgtaataagc
    19501 ggcgtcttta ggtcatgcga tacagtgatc gcaaagcggt ccaattcctg gtttttcagt
    19561 ttcaactgac ggacaagttc ttcacgttcc atttccgctt tttctctttc agcgatttcc
    19621 tccctggctc gaattaaact gcgcctgatg ttttccacta aaagatgcac tgcattgctg
    19681 gtaaccagga tgattgcgct ggcaaaaaga aaatcctcgg gcaggctggc caattctgtg
    19741 tcggctttgt aaagtcccaa catatcgcca aagacaagcc acccgaggca taaaatgatt
    19801 cctcctgtca ggtagggcag cactctgccg cgcagaatca accccgtcac aatcagaatg
    19861 attggaaaag ccatattcgc cacgtcatgg atcccgttgc caactgtgga gatgtaagtg
    19921 agtagtaata tggttccagt ggcaaaaata gagatcggca gagaaagctt tccgctcttg
    19981 atcatcagca gggatatgat gatgggcagg gtgcccgcca gaattaccag ggtttggatg
    20041 tacctgccct tgaataactg gttaataacc aataccagca gaatgaaaag ggaggaatag
    20101 gccacccatc gcaccgtttc agcgatttgc tcttcattgc cgtagacgcg tcttctcaat
    20161 tgcgaggaaa gcggttcctt gtagccagat ttatcgtcca tatcacacct gttgtgttac
    20221 gtgcgtgccg tcaatgtaaa gcagaaggtc gtgccttttc cagactctga ttcaacccaa
    20281 accctgcccc catgaaattc aatgatgcgt tttacgagtg caagcccgat ccctgttcca
    20341 tcagtcccag catccagttt gttgaaaagg ccaaagatgc gttcgtgata gagcgggtca
    20401 atgccaatgc cattgtcttt tacgaaaaat atctgctcgt tctcttgttg aaaagaacca
    20461 accttgacct cggggtttga gcggtttccc atgaacttga tggcattgtc gatcaggttt
    20521 tggtacacct cgaccaagcg gtggcggtcc acttccatgc tgggaagatc tttctcaata
    20581 atgaccttga ccttgttgct ggtcacttgt tcgtgaagca gccttgaggc ttctctggca
    20641 atatcaccga tgttcgcgtt ttcaggcttg ttaacgaatc ggccgatcct tgaaagttca
    20701 agcagctcgt tgagcagacg ctgcattttg ataactgcat ccattacgcg ctgaatatct
    20761 ttttgggcct tggcggtgtt tccattcagg atgtcttttt cgagatagcc tacaaacccg
    20821 ccaatggtga tcaacggggc tttcaggtcg tgtgagacag tgtaggtgaa gctttcaagc
    20881 tcattatttt tattttgtaa ctcgatcaat aattcatcta tttttgtgat gtcgtagagc
    20941 gtgtagagta cacatgcttc attattgact gtaatcggtg caaccgataa taaggctacc
    21001 cctgcttcgc ccgatttgcg cctgaatctc gccttgaatt cacgaatgaa accctgttct
    21061 tgaaggagtt gatgatgttt gttgtgttcg tctggatcag cccagaggtt taggtcgctt
    21121 gctttgtggt ttatggcttc ttcgcggcta aagccgattt cctgactgaa cgcctcgttg
    21181 atatccacaa ttgacttgtc ggaaaggcgg ccgatcacca tgggtacaaa tgtgtggtga
    21241 aaggttgttt ggaatttttc ttctgatgcg cgcaatgcgg tgtctatttt ctggcgttta
    21301 atggctgacc ccaacatgct ggcggtaacc tgcatggcat gtacttcggc ctttgtccac
    21361 gccatctcac ggatgcaatc atcgaatccg atcacccccc accatttgcc atctacaaaa
    21421 atgggtacat ccaaaagcgt ttttatatcc tggcggctgg gagtgtcctg ccatacttct
    21481 tcaaatatct ttgtggtgtt gtagaaaggt ttgccctgac tcaccacgga ataccaggct
    21541 gccagtcctt cttcaaggac ggggacgttt tgaaagtcgg gattatcaag ctcggaggtt
    21601 tgtcctgggc cagtccattc gtatttttgg ctggttacca gattgccgtt ttcgtcgagg
    21661 tggttttcga aaaggtaggc atggctggcg tgtgtagcct ctccaagcaa tttgaggagg
    21721 tcattgatct cggttttcca atcaggggct tccagcagcc ttacagcaga ctgggcaatc
    21781 gcatcaagga gtacatcgct tcgccttgga agtttttcct cgggggcatt ttgcttttca
    21841 tctgatgcgg attttgtctt tttcatggat attcaagccg cttttttgaa ttgggataaa
    21901 aaaatctcta ccactaaatg atagagattt tagcagataa acttgacctt acacgatcaa
    21961 actgcaacag gtgacggccc cttccgcttt tgccagttca gccacgttga cagcctgaat
    22021 tttgtatcct cttgcttcaa gttttttgct tgtttttgga aaggctgtgg ggaagataat
    22081 tccatcgcca acaggcaggc agtttgccgc aaacggttct gcggaatcca cctcgatcca
    22141 gtcaaagcct ttgaagtggg aggtgtccac ccagttgggg ttgatgagca gggtcttgtc
    22201 atccactttg gtgagggcgg tttttaggtg cagacaatca tgcatgtcca cagcggtaac
    22261 ggtgtagcca taatgatcga gcagttgctg aatttggcgt acagcatctg cgttgctgcg
    22321 agtggagttg ccgatgtaga tgttttttcc caaaacgagc acatcaccgc cgtctacggt
    22381 gcctggctct gttacatgga caagcgggcg gtacgacgat aatgcacgga tgatggattc
    22441 tgtttcgggt ttccgagaat cagccccagg tcgggtgatg accgccactt ctggcaggat
    22501 aaaggctgtg tcttccacaa ataccgagtc aggcaggtcg ggctcttcgg gtaattcaat
    22561 cacctgacaa cccagggcgg ccagcgcgtg aacatattcc tgatgctggg cacgagccac
    22621 attcagatcg atgggggtgc gttcaatgtg ggttacttcg cattcgttaa agcgcggact
    22681 gattttgcgg gtgatcgcta tgggcattta gttttctccg gagcggtagc ggtcaataag
    22741 agcttttgtg cgcgggtcct tcggattctt gaagaggtca tcaggggtgg cgtcttcgat
    22801 cagttcacct tctgccatga cgatgacacg gtcggcaact tcacgggcga agcccatttc
    22861 atgtgtgacg acgatcatgg tcatgccttc gagtgccacc tttttcatca cgttcaacac
    22921 ttcgccaatg agttcggggt caagggctga agtgggttca tcgaagagca tgacacgcgg
    22981 ctccatggtg agtgcgcggg caatggccac acgttgtttc tggccgcccg aaagccgcat
    23041 ggggtattcg tcttttttga aaagcatgcc gacgctgtca aggttttgct cgccaagctc
    23101 aatggctttt ttgcgatcca ttcctttgac gatgaccggg ccttcgatca cgttttccaa
    23161 aacggtcatg tgagggaaca ggttgaattc ctgaaagacc atgcctgttt ttagacgcac
    23221 atcatgaaca agctggcggt gacgggaccc cttttcgccg ccttccacac gaacgccatc
    23281 cacctcaatg gtgccgtgag agggttcttc aaggaaatta agacagcgca ggagcgtgct
    23341 ttttcctgaa ccgcttctgc caatgaggca tacgacctgt ctttcaggaa cctcaaggct
    23401 gacatttttc agaacgtgca aacggccaaa atatttatca aggtttgata tttttacaat
    23461 ggaggatgtc atgttatcta tctcccttgg aaaggcgggt ttcaatgaca ccttgaacat
    23521 aggaaaggat gatggtcatg acccagtaaa aaatggcggc catgatcagg gcttcaaggt
    23581 tgtggaattg tgcgcgtcca actttgacgg cgcgccaggt caactcgtga acaaagcccg
    23641 tggctgaaac cagcgcggaa tctttggtca tggcgatgaa ttcgtttccc gtgggtggaa
    23701 tggcgaaacg catggcttga gggaggatca cacgcttcat tgtctgacct ggggtcatgc
    23761 ccagcgctgc tgcggcttct ctctgccctt tgcctacaga agccagcccc gcgcggaaaa
    23821 tttcagacat gtacgcgccg tagtttaatc ccagcgcaat gacgcccgcc caaagcccag
    23881 gcaggcggat atcaagctgc ggaagggcca ggaagaaaaa catgatctgc aaatacagcg
    23941 gggtaccgcg gatcaatgaa acataaaagg tgctgagcgc ataaatcggc ggaaacgtgg
    24001 aaagcctgcc gagcgctgcc aataaagcca gaaaagtggc gagaatgatg gaaaggatgg
    24061 aaatacgaat ggtttcgccc acgccgcccg caatgaacag catgttttcc ttgatgaagt
    24121 ttgtatctac attgatgggg ccgctgaata aaaatacaag caacagcaat aacacccccc
    24181 aggttagagc cacgtttgca cggaaatgac gctctttacg ctgttgcgct tcaaacaaca
    24241 attcggcttg tgttttaggc gcgccagatt ctgctttggc gaaaaggttc atgagaaaat
    24301 tcctccacag ggaattaata gcaaggaagc ggcatcacat gccgcttcct tttgttttga
    24361 acagtttact tgttcggtgc ctgggtcagg tcttcgctga accatttcat cgagagttcg
    24421 gtcaggcgtc cgtcaccatg catggcttcg aagattttgt tcacttcggc gatcaggctg
    24481 gctgtgggca gggtggagga tttgtcgaac gctgcggcaa ggtcttcaga gtacacagca
    24541 ctgccgagaa cacgaacggg gatgccggcg gcaatattcg cattcacaac ggtggctgag
    24601 gtggagtagg cggcaaagtc ggtgcggcca gcggcgagcg cctgggcgca ttcctggtca
    24661 gattccaacg gaaccacagt gatgcctgaa ggaggcttgg cgtagatgga actggcgggc
    24721 aagccaattt cgccgccatt cagccaggtt tcataggtgg tggaaacacc tgcacaaatt
    24781 gcctgaccag agaggtcttc aagagcctgg aactcagagt cgccaagtac tgcaacaacc
    24841 gcgggggtgt agtagtacgg aacgctgaaa tcgagcactt tttggcggtc agtggtgatg
    24901 gtcatggagc caacgctcat atcccatttg tcagcccagt tgccagcggt gagggtgtcc
    24961 caaccggggg tggcaaaaca ggtttcaaca ccgagcgcat cgccgacggc aatggccaca
    25021 tccacatcga agccctgcat ttcagcagtg gtcaatgcat cagaggggca tttggtgtct
    25081 gaagggcgtg agccttcggt gttcaggaag gactggggtt cataattcgg gtcggtcgaa
    25141 atgaggatat agccgcgttc cttgatggcg ccaagcaggt cggttgcggt tgagccgcca
    25201 ccaccacaag cggtgagcat gagagatgcc actacaagca ggctggccat tgtcaaaagt
    25261 tttttcatta tctcctccaa gagaaataaa aatgattggc aatgtttcat tgccacggaa
    25321 cgttttattc cgcgggttaa gcatagacta tttttagcat ttgtgcaagg attttgtgta
    25381 aatccgtaat ggtacaattg tgtatactat ttttcggagg caggcatgtc tgtaattttt
    25441 cccagcgcag aatggctcgc ggagttggaa gccaaattga attctgatga gaagtaccgt
    25501 gagatcgcca aaaattggga gggcgatatt tttttcaata tcgaaccaga gggaaatttg
    25561 aaggaaccgc tcacgttcta tctagacctg tggcatggtg cctgcagaaa agtggattac
    25621 aaccccgcgg cctccaccta cccaaaaccg acttttatcc tcaattcttc ctacggcaat
    25681 atcacggctg tgctgttggg taagatgaac cccatgaccg ccatgatgac catgaagttg
    25741 aaggttcagg gcaatatggg ttacatgatg cgcaacgtgc cgaccgtgct tgattttgtc
    25801 cgctgcgcgc aggaagtgac gacggatatt ctgtaatttt tcttgccagc ttcttttttc
    25861 acttgtgaga aaaaagaaga aagaattgat atatgctgcc gatattaaaa atcaacctga
    25921 ctaccaacac aactgaagaa tttgtcatcc caaaagggtg ggaacgggat tttctcggcg
    25981 gtgcatctct cgcggcgcgg attttatact caaccctgac aaaggaactt gacccgcttt
    26041 cgcccgaatc tcccttgttg ttcatcaacg gcccattgac tgggacaaac ggccccacca
    26101 caggccgttt tgtgatctgt ggaaaaagcc ccgccacaca gttatgggcg gaatcgcata
    26161 tcggcggttt ttggggaccc gaacttcgca aggctggtta tgacgggttg tggattacag
    26221 gaaaagctca aagccccata tatctctgga tcgaagaagg caggttcgag gtccgcgatg
    26281 cggcgcgtgt ttggggcaaa gatgtgtatg aggcacagga gatcgtcaaa gaggaagttg
    26341 gagtcaaagc cgcaagagtc gcggtgatcg gcgaagcagg tgaacgtgga gtttattatt
    26401 cctcgatctg ttgcgaccac gggcgcatgg cagggcggac gggtctgggc gctgtgatgg
    26461 gggctaagaa tctcaaagcg gttgcggttc acggaaaaaa caagatcgaa gtgaccgcgc
    26521 aatacgcaca ggcgcgttct gaggccaacc gaactttgaa acaggataat gaggcaaaga
    26581 tcctgcatga acttggttca tccggtgtgg caaactacgc cgagtatttg ggctccatgc
    26641 cagcgaagta ttatcacaaa ggttcgttcg aagaggtgga tcatatttcg ggttcgaaaa
    26701 tggccgaaag tattttggtc agcaccagtg cctgccaggg atgtgtgatc gcttgcggac
    26761 gtgttgtaaa tttaggcgac ggagcaaaac gaaaaggccc cgagtatgaa acgatcgctg
    26821 ggtttggccc caatcttttg atcggcgacc tgaatgatgt ggttcgcttg ggggagatgt
    26881 gcgataaata tggaatggat gccatcagcg catccaatac catcggtctt gcattccact
    26941 tgtttgaaat gggcaagata acgcttcagg atacgggtgg gctggacctg acatggggaa
    27001 gtttccttgc tgcgcggcag ctgcttcgaa tgattgcccg ccgcgagggg atcggcgacc
    27061 ttatggcgca aggctctcgg gctttgggca aagcctttga cgctgaagat gaggccattc
    27121 aagtgaatgg gcttgaaacg gcatatcacg atccgcgcgg cgtttcaggg atggcgcttt
    27181 cttatgccac cagtccgcgt ggtgcctgtc acaaccaatc agattatttc tttgtggatt
    27241 tcggccatac ctacgacgag atcggcatca cctattttga tcgccatgcg caggccgaaa
    27301 aagccgccaa tgtggcaaag catcaagact ggcgtactca tttcaatgcc gttttgatct
    27361 gtatctttgc caatgtgccg ccagaaacgc aggtgcagtt actcaatgcg caattggatt
    27421 tgaattggac cattgctgat ttgatgcgtt caggcgagcg cgggtggaat ctgaaacgcg
    27481 tcatcaacaa tcgcatgggg ctcacccgtg caaatgataa attgcccaag gcctttcttg
    27541 agccgcatca agaaggtggc gcagcgggat ttgtccctga tattgaaggc atgctttcgg
    27601 cttattatga aactcgcggg tgggatgtaa ggacaggtcg cccgaccaaa cagaaattga
    27661 ttgaattggg aatgtcagat attgcaaacg acctgtgggg gtgacaggtc gttttgtgat
    27721 ccgtatttta tttttcctcg gttgcgttca accgccagac ggccagaaca aaaaagacaa
    27781 gtgcataccc tgccagtgcg aggcagggta ttacaactga ttcaaaccca agcccgcggg
    27841 caacgatgtt ttcaaaaccg tccatgcccc aggctacggg tgagatgtgg ccaatggcct
    27901 gaaaggtttc tcctgtaaat tccaatggca cccatgcgcc gcccaaacca gagaagacga
    27961 acatcggtac cagcgagaag gtaatggcct gttcctcggt gcgtgcgagg atcccgatca
    28021 gcaagcccag cgcggagata cagagcgctg ccgtaaaggc tacaagcagt gttgccgaag
    28081 gcaggcgcag ataatccact ttcaaagcga attgaccgaa acagatcaag atgaggaact
    28141 gggtaaagat aagcgagaag atcgccagaa aatgacccac tatgatttga atacggcgcg
    28201 tgtccgtggt cagtaacctt tgcagtgtgc gggatttgcg ctcggtaaca atgacctgag
    28261 ccgcggtcag caggcctgca atggcaaatt gcagcatcat gcctggtgaa gtatgtgcca
    28321 atgcgttgtt ctcttcgccc tctttgatca caagactgga cgtttcttca atggcgatgg
    28381 gcggctcatc ccatgcggct agaatttcat caaaggcgta attaaaaggc acgccttcca
    28441 tcttaaattg ctccatgatg ccagcggtac gcactgctcc atccacacgg ctggctgcgg
    28501 tgaatatttc agcctcgatc gtcatccaag cctgggtatt tgtatccgcc agcactgtga
    28561 ggcggatcgt tttatccttc aacactgctc tgccgtaccc atcggggaca atgatggcgg
    28621 cggcaagttc cccatttgca accagttttt ccaggtcaga ggcggaacgg aacacatttt
    28681 catccagacg aatcacttca gagtttttga gtagatcgtg taattcctcg gtcacccagc
    28741 tgtcatccag agagagatat ccaacaggca cgcgcgaatc ggattcgttg ccgccgaacc
    28801 ctccaaaggc gtatccgaac aaaaacgtga aaaggatcgg catgatgagc aggaacatga
    28861 aagtcttgaa gtcgcgcatc aattgcagca ggtcttttaa agcaatgtcg agaatgcgga
    28921 tcatgtcggg ctcctatgcg taccgcttgt tgaaacgcca tacgccgatg gcaaagaatg
    28981 ccgcacccca caaaaacgag accagcaccg tcagggcaat gctgttgaag gattcgccgc
    29041 tcatggcttg catgagtccg cgaacggccc agccctgcgg caccagcagt gaaatggtgt
    29101 cgcccatttt gttggcaatg gccgagttca tggcaaagat cttgatcatg cccatcatgc
    29161 cagtcaccgt caaaataccg ccgaataaca ctcccccctg ttttgtatct ttcaaaaatg
    29221 aattcacaaa aataccaaac gatgatgcgc tgacaataat tccaaccgcc atcaatgcca
    29281 ctgatgtaaa ttcaccccat tggattccaa agatcaaatt cgcggcgatc aacagcacaa
    29341 tgatctgcac cgaaacggtc atgaacactg aaagaagttt gcctgtcaaa atggtggctt
    29401 gtgaagtggg ggtggtaaag agtcgcggaa gtgtgcgctc ttcttcctct ctaagaatgc
    29461 tttgcgcggt ggatgtccct gtgtagtagg catagaaaac catcatccca cccatgattg
    29521 gaccaataat gcctaataat agattctgac tttcatccgc ttccttttct ggaccgttgg
    29581 ctgggttgcg cacctcaagc agttccgcct ctggatcatc gctttctgcc agtgaaactt
    29641 cgagatattg gtcaaccact tggcctgtca aaacaagctg ttcgggctgt gcttcatcca
    29701 tgaagaagtt gatggcaatc ttcactcccg acatgccgtc cataaaacga ttgaggatgg
    29761 atcgcatcac agcgggcccg agggtgagcg ttgggtcttg ataaaactcg atcacagctt
    29821 ttccatccac atcggcgaat tgatgcgaga aatcggctgg aataatgatg gctacctgtg
    29881 attcctggcg gtccaccgca ctgcgggcgg attctgcgtt tggtgccaga gagacttcga
    29941 tcaagtctgc catgtcatcg ctctgcagaa tgctgacaat gagatcgccc atcgagtcgg
    30001 cttcttttcc accagggatg tttttcgggt tcacttggaa cttcggcccg ccttcatcaa
    30061 ggttggcaat gatcacattg gttttcggca gggtaaatcc gccttcgtcg gcgatgttgc
    30121 caaacataaa ataaaacatg ccagtgacca gcagcggcac accgaacata aagacaacgg
    30181 cgaatgtgct gcgaaaggaa cgggtcatgt ctttcagggc gatgtcgaat gctttcatga
    30241 caaaattcct taatcgcgaa gtgcgcgccc tgtcaggtgc aggaaaaccg cctcaaggtt
    30301 cggctcgctg atgttgaccg aagtgatccg tgcagaatat tttgtagccg tttcgaagag
    30361 gtgaggcaag gtcaggttgc tgtcttgaac caggacgctg atcaactctc cgttaagggt
    30421 gatgcgggaa acgtcagcca gttccttcca ggcggggatc acggtatcca tgtttccgtt
    30481 cagcgtcagg tcaatgcgtg tttgctgacc gaccattttg accagttcat cgtgggtgcc
    30541 gtgtgcgatc agctgaccat gatccataat gccgatgtgg tcagaaatct cctgtgcttc
    30601 ttccatgtag tgcgtggtgt acagcacggt catgccttcg tttttcaaca atatcacgct
    30661 gtcgaggatg ttgcgccgcg attgcggatc aatgcccacg gtgggctcat ccatgtaaat
    30721 gaccttgggt ttgtgcaaaa gcgccacacc gatgttgaca cgccgtttca tgccgcctga
    30781 atatttccca acccgttcgc tggcgcggtc acgcaggcca atgacgtcca acacttcatc
    30841 aactcgagat ttgagggcgg ttccacgcag gccgtacatc tttccccaaa agaccagatt
    30901 ttcgcgcgcg gtcaggtctt catacaaggc gatatcctgc ggcaccactc ccagcactga
    30961 tttgactcgg cgctggttcg tggaaatgga atgccccatg atttgtgcgt caccttcatc
    31021 ggggtgaagc aggcaggaga gcatggagat ggtggtcgtt ttgcctgcac cattggggcc
    31081 aagcaggctg aaaatctcac cttgatctac tttgaagctg acaccttgaa ctgcatgatt
    31141 ttccccaaag gatttgcgta gtccttgaac ttcgatcgct ggcacaatgg tgttcattag
    31201 aggctctcgt tttcagcgat atatccttat cgcggaattt gggagtggct aaaaactggt
    31261 tttgatttca cgaaattagc cactcgtcgg aagtaaaaac tgttctttct atacgcagat
    31321 ttacagaatt cgtttcactt tttcagccaa tccagtgcgc tttcgtaatc aacaaagagc
    31381 gaggcgaaaa agccgttatt gcggcagaca gtttcgtaaa acttcaggtt ggtttccagt
    31441 tttttaggca caatcagggc tattttaaat tgacgcggaa tcccaagctc ggcatagcgt
    31501 ttgggaagct cgtaaatatc catggtggcg agcgcatctt ccgaaatgtc gcgatggtcg
    31561 agcaggcatc gcatacagtt gtgttttctg ataagttcaa ctccttcgtt gcgcatcaaa
    31621 gtagcggact tgatctcaag tacgccattg gttttaatga aaatgatctt ctccgcctcg
    31681 tcatagtaca ttttccaatt catggctgcc tctctccctg tttttattcc tgacgatatt
    31741 gttattatat aatagagttc ttgcaaaagt ccatttttgc cagatggtct caaagacttt
    31801 acaagaggtc ggctggttta tcgcggcaat tcccaattaa ccttggccga tttggggcaa
    31861 ctcactgatc gcctgaaata aaattggagg ttgaatgaaa ctcgttactg tggctcagat
    31921 gagagccatt gaaaaagaag cagatgccaa tggggtttcg tatgcacagt tgatgcaaaa
    31981 tgcagggcgc ggccttgcag aagtggtcca tgctttgggg caggagaatg gctgggagga
    32041 agttacagct ttggtgggag caggcaataa tggtggtgat gcacttgtag ctctcacatg
    32101 gcttgcgcag gcaggctgga ccacgcatgc atatctggtt aggcgtgatt cggccaacga
    32161 tgacctggtc acggcgtatt tggcggctgg gggagaagtg gcggattttg aagacgaccg
    32221 agcgctagcg gtttttctta atgagtcaga tgttcttttg gatggcctgc ttgggacggg
    32281 gatcaggctt ccgcttaaga aagaggcggc gcttgtcctt tcaaatgcaa aagagatgat
    32341 ctctgaaatg gagattccgc cttttgtcgt ggcggttgat tgtccatctg gcgttgattg
    32401 tgacagcggc gaaatcgccg aggagaccct cccagccgac ctgacgctta cgatggcggc
    32461 agccaaatac ggattgctca aattacctgc attcgaattt atcggcgagt tgcaagtcgt
    32521 ggatattgga ctgcctgatg gtttgagttc gtggcaggag gtcaacaccg aggccgcgga
    32581 tgatgagctt gtcgctgtgc ttttgcctga acgtaccccc gcttcgcaca aagggacatt
    32641 tggtacggct ttcgttgtgg ctggttccac ctcatacagt ggggcggctt tgttggcagc
    32701 aacagccgca taccgtatcg gtgctggcct tgtgaccctg gctgtcccag agcccttgca
    32761 cgttgccctt gctggccgat tgttggaagc aacctgg
//
LOCUS       DAOQJC010000040        31706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_615_length_31706_cov_7.972955, whole genome shotgun sequence.
ACCESSION   DAOQJC010000040 DAOQJC010000000
VERSION     DAOQJC010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 31706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31706
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_615_length_31706_cov_7.972955"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PLE39_08020"
     CDS             complement(<1..910)
                     /locus_tag="PLE39_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68311.1"
                     /translation="MTYEQQFLNALKSIFIGAKVEGDSGYINLMKIKASYFEKGVFPV
                     LMKDIDAACKPFEKGFREEMFDKLYDFFQHYFSESGSIYFRNTAQHHNIYEKVYTDDR
                     DVMLFWKTHMLYYVKTDRLFNSMEIEVDGEKFFFDVSEMQLKRSNEKRELAYTFKSIK
                     NGVINLTVGYSERGRKTKIDEIVKAVKDKGMTLGEETLTKAFAVFQKQSEVDYFINKN
                     AKAFLQEQFDLWMYQYVFKGESVFNETRLKQLQAIKEIAYKIIEFIAQFEDELVKVWN
                     KPKFVLNSNYIITLDKITDEKLLKKAF"
     gene            complement(1128..2252)
                     /locus_tag="PLE39_08025"
     CDS             complement(1128..2252)
                     /locus_tag="PLE39_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68312.1"
                     /translation="MKENDQSRFIRWGVPGWTMLLAFASFTLLDILLSPSSSKNSLVD
                     VIKTIVAIISLNAAASAAAALLVAAAGIPLGFVIYQAYFYIRWNSPFSRDGFFPPFIV
                     GRLNDLERTMEGVHKHFIVGDDKWRREWTNNPLYNSDHGLKWRYIENLFTEIVQYMDS
                     KLSGLSLHVRYRYLMDLMHTLGAALFGIYLGYLGYILLKVKTDDISLSIILSISIVCL
                     FFLMILLEMEDKVKKGKVKPVPKDKIHYLVVTPIKRFDLFQLSNPSANYLLFLFTLLY
                     MGSPSPYTVTYTPSHFVFRIAVVVAVVLAWLIPKLAYPKRVIISEILTLAVLEILSVM
                     ASKYINDWSAFKPDWWNIGWCILFFSCNKYDLHKKPSKHT"
     gene            complement(2468..3019)
                     /locus_tag="PLE39_08030"
     CDS             complement(2468..3019)
                     /locus_tag="PLE39_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68313.1"
                     /translation="MAKVRNNIIIQGLSGSLGDQLVIKQDKAGRTIVAVPPSVDPNRT
                     FSAAQLERQEKFRDASVYAKGAKDEEVYVEKAKGTPQTPSNVAMADWFHAPEVKEIDT
                     EAWSGQAGQVIRIQAVDDVQVTQVNVVITDAEDAVMEQGAAVRAEGAWWAYTTTASVT
                     GEAKIVVTARDLPGNIASLSWES"
     gene            3375..4625
                     /locus_tag="PLE39_08035"
     CDS             3375..4625
                     /locus_tag="PLE39_08035"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE68314.1"
                     /translation="MRFNFFQQSDTPQKYRANFTNLYLDIAWFGVLSGTSVNFLNVYA
                     TRLGATSFQIGLLAAMAAVVNLFLAIPAGRWIEKRHTGRAVFWSSVLFRIGYLFWIPL
                     PWLFDEQGQIWALIILAFLMAIPLTPLGVGFNALFAESVPERFRAQVAGTRNVTFAIA
                     YMLTSLIAGAILKNLPFETGYQVIFAIGAFGAAMSSYHIYHVTPLEETAPLPAIPTPA
                     PTPQTDSPRSLHTTLRLDIWNTKFRNVLLVLFFFHLTHFLPTPIYPLYNVRILNLNDN
                     NIGIGTAIYYLTVLIGSTQFRRIAHRYGNKKVTAWGAAGMAIYPLLLAFSGNIWQFYG
                     LSFIGGFLFAMINGAYINYMLENIPPNDRPSHLAWYTIILNTAILAGSFLAPAIAETI
                     GLANALIFIGILRLLAGLSVHKWG"
     gene            4690..5151
                     /gene="smpB"
                     /locus_tag="PLE39_08040"
     CDS             4690..5151
                     /gene="smpB"
                     /locus_tag="PLE39_08040"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNE68315.1"
                     /translation="MTEDIKVIATNRKASFEYFLMEKFEAGIVLQGSEIKSVRAGQVS
                     IQEAYVDISDGEQAWLVEAHIAPYEQAGKYFNHEPKRKRRLLLHKKQIRELWNNVRIK
                     GNTVVPTRVYLKDGRAKIEIALAKGKKAYDKRATIAKRDEARSNERATRVR"
     gene            5225..6007
                     /locus_tag="PLE39_08045"
     CDS             5225..6007
                     /locus_tag="PLE39_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68316.1"
                     /translation="MPPSTIGGINLLPESEKRAIYSRYIPTVLLEKYNLPPLTSAAGY
                     NLLQFRFATGSTDVEMRLFHQVDFPDPILYAHLTDTLNGQIHVLLYILNDPASPRFNV
                     DRMPDGSPTKFGTLQRNIPSETEALEAGLAPGQVRRGLRLLGQAIEAFEGFVAALGHD
                     MYFVEPLYYHNAVIFERYGFSYQVGKRLMDSIHAGFQNGGDLAAKLDGSTPFRHPEAA
                     NGIRKRSWAIHDGLLDEPFTNVTMYKRVGKSAGINTTPDCKW"
     gene            complement(6272..6721)
                     /locus_tag="PLE39_08050"
     CDS             complement(6272..6721)
                     /locus_tag="PLE39_08050"
                     /inference="COORDINATES: protein motif:HMM:NF023075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group I intron-associated PD-(D/E)XK
                     endonuclease"
                     /protein_id="HNE68317.1"
                     /translation="MNTKHKGDIAEQAAVLEALKRGWGVLHPVGDNLPYDLVFDVFGV
                     LIKVQVKCAWFDVPSQNYVVDNRRTKTNRRSMIRDEYTETDFDFALVYLPDCSLFYIF
                     PVNVFISYGSEIHMTETDKRQRRPRSFEYRDAWKLIQQWAANEETHS"
     gene            7022..7678
                     /locus_tag="PLE39_08055"
     CDS             7022..7678
                     /locus_tag="PLE39_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE68318.1"
                     /translation="MKTTTLLVIEVHHSEIPSFAPDLQKKGFDVQIAPNGSKAVARLK
                     DVSPSLVVINAASLRSTGLRICQAIREKDAKLPIILIVENDGDVSKDAADAVLGLPFT
                     VQKLVNRIKPLLPGDGKNVLHVGPIRLDLEKRRVRCLGKSTKLTPRLVTLLHLLMDKH
                     GEVVEREPLFKKAWETNYTGDTRTLDVHISWLRRAIELDPDNPKFLKTIRGVGYRLDI
                     "
     gene            complement(7667..8899)
                     /locus_tag="PLE39_08060"
     CDS             complement(7667..8899)
                     /locus_tag="PLE39_08060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNE68319.1"
                     /translation="MPILNWKGKHPAAPEPASLIQDAILYPKGHGYPKTRPSGRVILG
                     ENLSVMAALLPEYEGRIDLIYADPPFFTNRKFSARIGRGEDSRKPHKWKLAEGYHDAW
                     PDLDSYLQFLYERLALMVRLLSPKGTLYLHLDWHADAYARLILDELFGSTGVFINEII
                     WAYHGPSPIKTAFNRKHDTILSYAKSGDYTFNVDAVREPYNPSTINTFKSSRKAGFGK
                     VPNLERGKVPEDWWYFPVVARLHNERTGYPTQKPEALLERIILASSNKGDLVADFFCG
                     SGTTALVAAKHGRKFITSDEAVRAVNTVRGRLVEAKSVFTVDRDESIKSVFLDGAKKV
                     KAHVLENSIRLETSLDVDFWEVDPDWDGKTFKSAVQAHRHARSGEIPLELEIKIGPKH
                     NACIRLVTVQGTQYQLYI"
     gene            complement(8959..10047)
                     /locus_tag="PLE39_08065"
     CDS             complement(8959..10047)
                     /locus_tag="PLE39_08065"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNE68320.1"
                     /translation="MKSQLSRRDFLKLGGLTLGGLAFTPFLPGLTDFDDGFVVRIATD
                     QMPVRSEPKDGSRIYQTWYRDDLVHVYEEVTAEEPKHNPVWYRVWGGYLHRGRLHRVK
                     TVYQVPMSSVPEGKRVLTEASVPYTTPYRFTKARGWEALNPPIYYGSVHWVDGVEEGP
                     KMADYTGAWYRIYDELDSNVPYYVPAIHMRVLPSSYFEPISPNVPYEEKLIEVNLSTQ
                     TLVAYEYGKSVFQTNISSGIPGGGLGGDKGISTTTPNGKFTVLDKVPAKHMGYSYFSI
                     GKGGDVTTDESGYVLPGIPWSTFFTPVGHAFHGTYWHENFGQEMSHGCINMRSNEANW
                     IFRWVQPNHSPEAISNHRGRGTMVEIHY"
     gene            complement(10173..10415)
                     /locus_tag="PLE39_08070"
     CDS             complement(10173..10415)
                     /locus_tag="PLE39_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68321.1"
                     /translation="MDSGLIGKVEKAKRYAEDRHRFHFNQFDLDFHGDNSEHHVSYNN
                     GVFNCDCEFFITHKRCGHSMALEILLKDMITESVAS"
     gene            complement(10780..13728)
                     /locus_tag="PLE39_08075"
     CDS             complement(10780..13728)
                     /locus_tag="PLE39_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNE68322.1"
                     /translation="MLISRASRLKFNFSVDFFRPDGHVTFADLTAARAFAAQMSTPRT
                     DPVPGSDLYAFYLLDEAYHIILKHYFSRYTGVMGRAMGVLQSNLGSKYDLTLSKFIEE
                     FPPLSVFLGQTTAGVYLATKLPNFGDFGHGVRAAAVEEMLLINNSNNNPALSRYKVLI
                     DESVMNGSAYKELVQLLLDFFARQPGFGNGEFALGETLNELLLAPIKASPDSLEGQLQ
                     FIIQKWGHLLGEAFSAKLLRGMDFLREETIRLQTRQIDPSQKVETYVPSFVGSEYAEY
                     ERYSPDKDWMPRLVLLAKNSYVWLGQLSKKYQREISALDQIPDEELDLLASRGFTGLW
                     LIGLWERSRASQIIKQRMGQADAVASAYSLHSYDIASDLGGWNALNNLRSRAWARGIR
                     LSADMVPNHMGIDSTWVVEHPEWFLSTSNPPYPSYSFRSENLSNDLRVGIYLEDHYYD
                     KTDAAVVFQRRDLQTGDLRYIYHGNDGTSFPWNDTAQLDYTNPVVREAVIQVILHVAR
                     NFPVIRFDAAMTLAKKHVQRLWFPEPGAGGAIPSRSQYGMTKSEFDEKVPEEFWREVV
                     DRVAAEVPDTLLLAEAFWLMEGYFVRTLGMHRVYNSAFMHMLRDEDNAKYRLAIKNTL
                     EFDPQILKRYVNFMNNPDEKTAVEQFGSGDKYFGICTVLSTLPGLPMFGHGQVEGFAE
                     KYGMEYRRAKLDETPDEGLLRGHEWRIFPLLHRRHLFADVEKFLLFDFYTSSGNVNED
                     VFAYSNRHNDERGLIVYHNRFANTSGWIKTSAAYIDKSSGRTLRKNLAEGLSLPRAGY
                     VIFKDCSSQLEYIRSCKDIWEKGMYIELGAYQCHAFVDFRFVGDVEWKTICDQLNGAG
                     VPSMQDKWKEMFAAKEETSVVEKTAKKKRVVKKAVTKKKETALAKAKKSPVKKKATVK
                     KPAKKKVAPKAKKAVETKPAAKAKKIVLTSKKAVKKTTKPKKGVESKKPVASVKTSKP
                     VRKKSSTKTK"
     gene            13784..16045
                     /locus_tag="PLE39_08080"
     CDS             13784..16045
                     /locus_tag="PLE39_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE68323.1"
                     /translation="MNTSPTLFVLLLLLGALYLPIIFFAIQRRDDGHGISIWLAAFFA
                     LLAMVLNVAEAIEASQGGTLLFQEVQIYIAFTLIAILMLAFQNFMRYETWWVWVGVWT
                     LWMLGLALILTNALKLPDVLWSNGTLTLPRERLGPSWAILGWLIISIALVISISNAGK
                     QARQQLFRNRLNYWWPTVFLLFVNDVLLFSNIMIAGQPLRLMAAVLMVYVIGTHNVPD
                     LRQIFRRILAYVAIAVVIVGAYAAGYLLIEAVFGNSKNFNPLFAGAFIALLLTLIFAP
                     MLNLVSRAVNNWVKGDQYNPSHTIHKYSESISNILDMDRLASIAVGIILEAMQIERGF
                     LFLVDSDREADGHRTYRLRSARSPEERQMIAVELDENGIIASYFIREQKPLLQYDLDL
                     LPAFRAASPFERDWFNQLHTEVYIPIFAKKQWIGLLAFGPKLSRNRYTSEDLVTLSSH
                     ANQTAIALENARLVDNLMRLNNELRQARRALEKNNRDLERIDQAKSDFISIASHELRT
                     PLTVIKGYTEMLLEDTSLDPNYKQIMNSIHDGTIRLHEVMDSMFDIAQIDARSLKPHI
                     QPVEIGHLLQEVCIEQASSIKERKHSLSIDLPSLPHLKCDPNLMRKLFHHLIRNAVKF
                     TPNNGKITVTGHSIPAIIDLPNGGLEIVIADTGVGVDPNFREIIFTKFYQPGELGKHS
                     TSKTRFKGGGAGLGLALAKGIVEAHGGRIYVESPGYDEINFPGSQFHVILPLTKPEEG
                     EEQKTSTPVQFQV"
     gene            16207..17469
                     /locus_tag="PLE39_08085"
     CDS             16207..17469
                     /locus_tag="PLE39_08085"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNE68324.1"
                     /translation="MPGKSHQELLKQYAEAIVKVGLNIRAGQRLIITLAATRGVPYQF
                     APLVHEVTKAAYAAGAKYVEVIWSDEEMMRLRAEHAPKDSFSEYPTWQVDAILKMIEN
                     GDALLSIGGSNPDLLGSLDPEKVGAMQRTHLQYYSKVSEKVTTNAINWCVVAAAGETW
                     AKKIFPDLDAQTAQQKLWDAIFETTRIDQPDPIAAWETHIVNLRKRAKYLQAKQYTGL
                     HYSAPGTDFTLGLPSGHQWISAQSMAQNGVMFTANMPTEEVFTLPDRNRADGIVTSTF
                     PLSYGGTLIEDFQVTFEKGRIVKVSARKGEAALQRLVDTDEGSHRLGEVALVPASSPI
                     AKRGHLFYNTLYDENASCHIAIGRAYRFTLTGGEELTDEEFVSAGGNVSLNHVDFMIG
                     SPKMDIDGIKKDGSREPVMRQGEWAIEA"
     gene            17534..18775
                     /locus_tag="PLE39_08090"
     CDS             17534..18775
                     /locus_tag="PLE39_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNE68325.1"
                     /translation="MDDQEFNQLLEKYAEVVVKVGLNLRNDQHLSIRGNVDDAPFIRK
                     VAETAYKAGARYVDVLWDDENIKRTRLQHADPETLTTLPDWLYNRYEEYSKRGDAELA
                     ILSSNPDLYNGIDSDRIAITRKTLYQKIVEPLRKYNNYANWCVVSTAAPAWAQKVFPN
                     LPEKEAQTKLWDAIFRACRIHTPDPVKAWEEHIQKLDKYKNYLNAKRFASLRYKAPGT
                     DLTIGLPEDHHWGGAQETFKNGITGTVNIPTEEVFTMPHKNKVDGLVTATLPLNNQGV
                     LMEEFSIRFENGRAVNFSAKKGDADLRKLIETDENAGRLGEVALVPNSSPISQSGILF
                     YNTLFDENASCHLALGSAYKSSMQGGVDMTDEEFAARGGNKSLIHVDFMIGSAKMDID
                     GIQADGTREPLMRNGEWAFSV"
     gene            18818..20062
                     /locus_tag="PLE39_08095"
     CDS             18818..20062
                     /locus_tag="PLE39_08095"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNE68326.1"
                     /translation="MNTAEFQKSLEKYADVIVKVGINLRKGQNLTISASITDYPLVRA
                     ITKSAYASGAKLVTVFYGDDEVFHTRMKMSSHKNLTEVPSWIVPAYTSIVKNGDAYIG
                     ITSDNPDLLADVDPEAIAIYRKTMGTKMRKLTQAVMRDAINWCVISYPAENWAKKVFP
                     KTSTKQAQEKLWEAIFKTCRVDQRDPVAAWEKHIKDLSLRSAYLNKKRYAALHYKAPG
                     TDLTVGLPSQHKWVAARSKSQAGIDFTANLPTEEVFTMPHKDKVDGFVAASRPLVIAG
                     TTVEDFTLTFEKGRVVKVTAKKHEETLRKLIETDEGAARLGEVALVPNSSPISQSGIL
                     FYNTLFDENASSHIALGAAYPFTMKDGNKLSQGDFQKRGGNHSIIHTDFMIGSKDMDI
                     DGILANGKREPLMRKGEWAFKA"
     gene            20135..20785
                     /locus_tag="PLE39_08100"
     CDS             20135..20785
                     /locus_tag="PLE39_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68327.1"
                     /translation="MPANSPKKETPISKDSMDLLIAEYNYIAATAAQSNEDRARVSSF
                     YLVAVGSLVAALFSTQFLNNVFNPDLINILFSILFLLLTLLGTTTILQLTRLRVAWFE
                     SLIAMDRIKEFIIARDKSLAGAFRWRIQNAPEFFKPNSVSFLQTVEVAILSALTFGTG
                     VFFFQKGIQYNCAPCNWAYSIALGLLAFFLQIWVYKKQLVLEKKRLQKEFSNNHDS"
     gene            20775..21347
                     /locus_tag="PLE39_08105"
     CDS             20775..21347
                     /locus_tag="PLE39_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNE68328.1"
                     /translation="MTRNYLGQPPTAFQRRPHLTRDDEWIAALLKVAQVGHIASAYEN
                     QPFINPTTFWFDEENHQIVFHSNIAGRVRSNIEANPKVCLEVSELGKLLPSNVALEFS
                     LQYRSAVVFGEARLVSEPNEAKQLLYGLISKYFPKMTAGKEYREITDKELRATSVYAI
                     KIESWSGKENWEARADQSDEWPALDEKWFE"
     gene            21360..21608
                     /locus_tag="PLE39_08110"
     CDS             21360..21608
                     /locus_tag="PLE39_08110"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HNE68329.1"
                     /translation="MPDDFRTEEFKVDGEKLIAKIKELIHEGNIRRIIIKDKEGKTIM
                     EIPLTFGVVGVLLAPTLAAIGAVAALLTEATIVVEKSA"
     gene            21620..22198
                     /locus_tag="PLE39_08115"
     CDS             21620..22198
                     /locus_tag="PLE39_08115"
                     /EC_number="2.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF040696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-S-isoprenylcysteine O-methyltransferase"
                     /protein_id="HNE68330.1"
                     /translation="MAIFKLVFWAAIIAEMVIRAPISRQQRKEAKSEQRVSAQEKTIL
                     GFLFVGMFLLPLIYSTTNWLDFANYSLPNWAGWLGVVLMGFALLIFWRSHKDLGLNWS
                     PSLEIRKEHTLITNGIFGYIRHPMYASQWVWVIAQPLLLQNWLAGFLNFLIFIPFYIL
                     RVRAEEQMMLDRFGDEYRTYMQKTGAIFPKGK"
     gene            22201..22647
                     /locus_tag="PLE39_08120"
     CDS             22201..22647
                     /locus_tag="PLE39_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE68331.1"
                     /translation="MNRDFQIKNKTSREKMRELIEGMTDAELALPAVAGWTVAATFAH
                     VAFWDFRILHLLRLWRENEIVPSPYDVDAINEAQKPLCMALPPRTAAELALKAALEID
                     SEIEAASDDLVKKIQDANVQFKFERNGHREYHIKEIEQVLAVHRAK"
     gene            22839..23501
                     /locus_tag="PLE39_08125"
     CDS             22839..23501
                     /locus_tag="PLE39_08125"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNE68332.1"
                     /translation="MTIQYLSESTEMYLKAMLELSDREKVAVGRLAERLNVTPVSANE
                     MVRRLSEQGLMAHTPYKGVSLTAKGRKAACNVLRRQRLWECFLYEHLKIEWAKIYELA
                     CSLEHATAPEVTEALSVFLGDPKTCPRGNPIPAADGTFAPLEGTPLSMAPIGATVEVL
                     AVNATATDVLTYLFERGILPGKKITITEAAPLQGPLTLKAGGKETALGLSMAEFVIVK
                     TN"
     gene            23518..23763
                     /locus_tag="PLE39_08130"
     CDS             23518..23763
                     /locus_tag="PLE39_08130"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HNE68333.1"
                     /translation="MPSSTQLHLLQPGQRATIVQINGAGALRRRFVEMGIVRGETILI
                     ERNAPLGDPVEYFIKGYHLALRREEAAQIEVNLDGGN"
     gene            23763..24278
                     /locus_tag="PLE39_08135"
     CDS             23763..24278
                     /locus_tag="PLE39_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNE68334.1"
                     /translation="MANLTIALAGNPNAGKTTIFNLLTGLHQHTGNWPGKTVEKKEGE
                     IEHNGMSINIVDLPGTYSLTAYSPEEIIARDFIIQEKPDVVINVIDATNLERNLYLTV
                     QLLELDVPVVLALNMTDALQKNGTKINTEHLSQMLGGIPILFTAANKGRGISDLMTKA
                     IESARKIKETA"
     gene            24278..25963
                     /gene="feoB"
                     /locus_tag="PLE39_08140"
     CDS             24278..25963
                     /gene="feoB"
                     /locus_tag="PLE39_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNE68335.1"
                     /translation="MHPTPQTIFRLDYGETLETEIASILAIFESMKFDTGKYSKRWLA
                     IKLLEKDSDILEHIQSMQGGREVIQFAVERAKQVESTLGEEIDLLTADRRYGFINGIV
                     RKSLQRPLIDRITLTDRIDDIVTHKWLGLPFFFAIMYVMFRLVIDVSSPFLDWTDTLI
                     NGPIAHLFSFLLDLVQAPAWMHSLLIDGIVAGVGGVLVFVPGLLILYFFLALLEDSGY
                     MSRAAFVMDRFMRVVGLHGKSFIPMILGFGCAVPAVYATRTIASRRDRILTALLVPLM
                     SCSARLPVYVVFGLAFFGSKAGTVIWAMYALGILVAMLAGMVFTRTLLKPDSTSAFVL
                     ELPPYRQPAMKSILIHMWENTREFVRKAGTVILGASVIMWLLLNLPLGVTNQKDSYFG
                     KVSGFIAPIFSPLGFGNWQTSGALVTGFAAKEIVVSTMSLVYTGSETTANAEPATFRE
                     EIISTGKGFVEATINSGKILVSILPGIDLIPEDEQTEDTVLSIALRNEFTPLSAAALL
                     VFVLLYVPCVATLGAIKHEFGNSWAVTSALYQTAIAWCTAFLVYQGGKILGFG"
     gene            25968..26249
                     /locus_tag="PLE39_08145"
     CDS             25968..26249
                     /locus_tag="PLE39_08145"
                     /inference="COORDINATES: protein motif:HMM:NF020581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HNE68336.1"
                     /translation="MLGQLLQLLEDKENGLSLSEISRALNAQPTAVLAMIAYLVQKGK
                     LLEIGPDGGHCASCGLVSDCNLLAARGKRYLRAELRDGKSIPGYVAPIE"
     gene            complement(26352..27794)
                     /locus_tag="PLE39_08150"
     CDS             complement(26352..27794)
                     /locus_tag="PLE39_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE68337.1"
                     /translation="MTNQLPTILVIDDDPIVRQTIKTLLAGDDVRLAFAEDGPSGIET
                     AQKLLPDAILLDVMMPRMNGYDVCQALRADPSLSEVHIVMLTSLDDRDARLTGFMAGV
                     DDFITKPFDRLELHLRLKTITRINRYRRLMAERARFSWIVENSYEGYLLLSSDGTIQY
                     ANSSAQTFLHLPRECVGINITSHVVKHYSPYPTETWTAWLDTPAPCYLVQPETANARA
                     FWLLLEAIDMPVKSDNSRVVRVRDVTELLSTHHDMRKFHKAVTHKLRSPFSTLVMSMS
                     MLENRLQKLSESEIKGFVSLSLEAINFLAGDVNKVLDYIDTPLAMESNIPLEIRFLPE
                     MLKSIQNEQGLGNINLTIHPDLMTACLGITDYAFELVLGELIENSKKFHPDHAPQVDV
                     LVEPVEHENMVRVTVMDDGIRLTAEQLKWARMPYFQGEKFYTGETPGMGLGIPTITSI
                     IWQVGGEVVITNREDRDGLKVSLRIPLANE"
     gene            complement(27817..28479)
                     /locus_tag="PLE39_08155"
     CDS             complement(27817..28479)
                     /locus_tag="PLE39_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE68338.1"
                     /translation="MHPVTILLVDDHKLIRQGMRSLLETRKGYSVVGEAGDGQGGLEM
                     IERFSPDVAILDVMMPGLNGIEVTRLARQRGFKTKILVLSMFANAAYAVRALQGGALG
                     YVLKEADFSEILCAIDTVMDSRRYLSPEIAEEVLEMLLQTGNEKESDVLNLLSPRERE
                     ILQLVGEGKTNAVIADKLILSVRTVESHRFNAMKKLRINSHADLVKFAINAGLVTSEP
                     YI"
     assembly_gap    28711..28810
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28811..29245
                     /locus_tag="PLE39_08160"
     CDS             <28811..29245
                     /locus_tag="PLE39_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNE68339.1"
                     /translation="KHENLMKSYRKELWFNIPARRGFVNITAQVEACLEESGIQEGLI
                     LINAMHITASVFINDDESGLHQDYDKWLEKLAPHEPVSGYRHNDTGEDNADAHMKRQV
                     MGREVVVAVTEAKLDFGPWEQIFYGEFDGRRRKRVLVKIIGE"
     gene            29302..29826
                     /locus_tag="PLE39_08165"
     CDS             29302..29826
                     /locus_tag="PLE39_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNE68340.1"
                     /translation="MRLQNKLIAILIAEGVEDLEYYVPLMRLQEEGAKVISAGLDMKS
                     VHGKNGLVITPDATIESLNADELFAIILPGGWAPDKIRRYDVVKNLVRKINDAEKIIG
                     IICHGGSIAISAGIIKKGQRATGSTGIKDDLVNAGAVWADEAAFREGNQVWGRVVADI
                     PDFNRELVKALVEG"
     gene            complement(29812..30627)
                     /locus_tag="PLE39_08170"
     CDS             complement(29812..30627)
                     /locus_tag="PLE39_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNE68341.1"
                     /translation="MNKVILVLSDALRYDVAKNNMGFLGHLVETKQASLHKIVGELPS
                     MSRPMYETIHTGMPSSEHGIVANSIVRLSAKPNVFQTLKNAGKTTAAAAYYWYSELYN
                     RAPYHPVDDKETDDERLNIQHGRFYTEDEYPDVELFASAAHLIRKFSPDYLLLHPMGM
                     DYHGETFGADSKEYRNHAIYQDSKLAPLIMEWKERGYIVFVTGDHGINADGGHGGTTP
                     EQRTVPFFVIQPSGRGRGETGEVISHLQIAPTILKLLNVPIPESMKYPPLSLD"
     gene            complement(30699..>31706)
                     /locus_tag="PLE39_08175"
     CDS             complement(30699..>31706)
                     /locus_tag="PLE39_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68342.1"
                     /translation="VDDFNLDIEKGEFVSFLGPSGCGKTTTLRMVAGFEIPTSGKIML
                     DGADITDKAPNQRNVGMIFQSYALFPNMTVAQNIGFGLRVRKEPEATIKQRVEEMVSL
                     INLEKHANKYPYQLSGGQQQRVSLARALAIHPQVLLLDEPLSALDAKIRISLRSEIRA
                     IQKKLGITAIFVTHDQEEALSISDRIVVMNTGVMEQVGTPFEIYNFPKTRFVANFVGS
                     LNTADAEVVDPANGLVSMDGVQFESAEGTEGRKKGDKVKIAIRPERLSFAADQKKANV
                     VDATIENITFLGSVVRIQVKIGNTKFNMDTFNNPFLELPALGSKEQVTCSKEAVLVLD
                     E"
BASE COUNT         8352 a         8289 c         7240 g         7725 t
ORIGIN      
        1 caaaagcttt tttgagcagt ttttcatcgg tgatcttatc gagcgtgatg atgtaattgc
       61 tatttagaac gaacttgggc ttattccaga ctttcaccaa ttcatcttca aattgggcga
      121 tgaattcaat gatcttgtag gcaatttcct tgatggcttg cagttgcttc aagcgagttt
      181 cattgaagac gctctcaccc ttgaagacat actgatacat ccacaggtca aattgttcct
      241 gcaagaaggc tttggcgttc ttgttgatga agtaatccac ttcgctttgc ttttgaaaga
      301 ccgcaaaggc tttggtgagg gtctcttcgc caagagtcat gcctttgtct ttcacggctt
      361 ttacgatttc gtcaattttg gtcttccgtc cacgctccga atagccaacg gtcaaattaa
      421 taaccccgtt tttgatcgac ttgaaggtat aagccaactc acgcttttcg tttgagcgtt
      481 tgagttgcat ttctgaaaca tcgaaaaaga acttttcccc gtccacttca atttccatgc
      541 tgttgaagag tctgtccgtt ttcacgtaat acagcatgtg agttttccag aacagcatca
      601 catcgcggtc gtcggtgtag accttctcgt aaatgttatg gtgttgcgcc gtattgcgga
      661 agtaaatgct tccgctttcg ctgaaataat gctggaagaa gtcgtataac ttatcaaaca
      721 tttcctcgcg gaagcccttc tcgaacggct tacaagcggc atcaatgtcc ttcatcagaa
      781 cggggaatac gcccttctca aagtagctgg ctttgatctt catcaggttg atatagcctg
      841 agtcgccttc cacttttgcg ccgataaaga tggatttgag tgcgttgagg aattgttgtt
      901 cgtaggtcat gaggaatcct tgagaatttt gtcttatttt acccgactga taatgaagcg
      961 gggcggcaac aaggtatttt ttatcctttc caaaacttta ttttttgcgc ctttttaatt
     1021 tcggcgtttt tctttttatg ccgctggttt tttggggagg ttcaaatggc tgtatactgt
     1081 tggaaaagta acgattgatt gtgtaatttt gcatcgcgat aaggtcatca cgtgtgtttt
     1141 gacggttttt tatgaagatc atatttgtta caagaaaaaa aaagaataca ccagccaata
     1201 ttccaccaat caggtttaaa ggcactccag tcattaatat atttagatgc cataaccgac
     1261 aatatctcca gcacggcgag cgtgagtatt tcggaaatga taacccgctt cgggtaggct
     1321 aatttgggta taagccacgc tagaacaaca gcaactacaa cagcaattcg aaacacaaaa
     1381 tgtgaaggcg tgtatgtaac tgtatatggg gatgggcttc ccatatacaa aagggtaaaa
     1441 aggaaaagaa ggtaatttgc agaagggttt gataattgaa acagatcaaa tctcttaatg
     1501 ggcgtcacaa caaggtagtg aattttatct ttggggacgg gcttcacttt accctttttc
     1561 accttgtctt ccatttccag caatatcatc aggaaaaaca ggcagacaat gctaatggat
     1621 aaaatgatcg aaagggatat gtcgtctgtt ttcaccttaa gtaaaatata tcccaggtac
     1681 cccagataaa taccgaacaa tgccgcccca agagtatgca tcaaatccat taaatagcga
     1741 tagcggacat gtaaacttaa tccgctcaac ttactatcca tatattggac aatttcagta
     1801 aagagatttt cgatatatct ccacttcaag ccatgatcgg aattgtacaa cggattgttc
     1861 gtccactctc tcctccactt gtcatctcca actataaaat gtttatgaac tccctccatc
     1921 gtacgttcga gatcgttgag tctgccaaca atgaagggcg gaaaaaaacc atctctcgaa
     1981 aatggggaat tccatcggat gtaaaaataa gcctgataaa tgacaaagcc aagcggtatg
     2041 cctgcagcag caaccaacaa agcagctgct gcagaagcag cggcattcag tgaaataatg
     2101 gcaacaatgg tcttaattac atctaccaga ctattcttac ttgacgaagg ggatagtaat
     2161 atgtcaagta aagtgaaact cgcaaaagcg aggagcattg tccagccagg caccccccat
     2221 cgaataaatc ttgattgatc gttttctttc atgaatcacc tccctaatat atgatttttt
     2281 gatcgacctt caaagtatac ctcacatctt tttagcatga catccccact gccgcacaag
     2341 ccgccgtcct tggcggcagg ggcgctacgc atcttgaacg ggtgacagcc atctcagaaa
     2401 tgactgtcac ccttccccta tcccccatcc cccattcact atccactatc ccccatccac
     2461 tatcccccta actctcccaa ctcaacgaag caatattccc aggcaaatcc cgcgctgtca
     2521 ccacgatctt cgcctccccc gttacagaag ccgtcgtggt ataagcccac caggccccct
     2581 cagcccgcac cgccgccccc tgctccatca cagcatcttc cgcatcggtg atcaccacat
     2641 tcacctgagt cacctgcaca tcatccacag cctgaatacg gatcacctgc cccgcctgtc
     2701 cgctccacgc ctctgtatcg atctccttca cctcaggcgc atggaaccaa tctgccatcg
     2761 ccacatttga aggcgtctgg ggcgtcccct tcgccttctc cacatacacc tcctcatcct
     2821 tcgcgccctt ggcatatacc gaagcatcgc ggaacttctc ctgccgctcc aactgcgccg
     2881 ccgaaaacgt acggttcgga tccaccgaag gcggcaccgc cacaatcgtc cgccccgcct
     2941 tatcctgctt aatcacaagc tgatcaccca gcgacccgct caaaccctga ataataatgt
     3001 tattgcgtac cttggccatg ttgaaagtct ccttttttct tttcgtatag gtcttgaaat
     3061 gacattcctt cgactctcct tcgacacacc ttcgactcac tcacatctca aataggccct
     3121 ccttcctgcg cgactatacg cagaaccaga ttcccccgcg cacagaaaca accccgaacg
     3181 caaggctcgc aaatggcagg tcaaaaatat tcaaaatcat ccaaagtgga aaaccatcat
     3241 ccaagtcccg ccattgaaga aatcatatcc aaaaccccgt cagctttcaa gcccccctaa
     3301 aaaaactgta aaccccacca attaccaatt acccattccc catcactcac ccctaaatca
     3361 tatataatct ctttatgcgt ttcaacttct ttcaacaatc cgacaccccg caaaaatacc
     3421 gcgccaactt taccaacctc taccttgata tcgcctggtt tggcgtcctc agtggcacat
     3481 cggtcaactt cctcaacgtt tacgccaccc gtcttggcgc aacgagtttt cagatcggcc
     3541 tgctcgccgc catggcagcc gtcgtcaacc tttttctcgc catccccgct ggccgctgga
     3601 tcgaaaaacg acacacaggc cgcgccgtat tctggtcctc cgtcctcttt cgtatcggct
     3661 accttttctg gatccccctc ccctggctct tcgatgaaca aggccagatc tgggcgctca
     3721 tcatcctcgc cttcctcatg gccattccgc tcaccccgct cggcgtcggc ttcaatgccc
     3781 tcttcgccga atccgtcccc gaacgattcc gcgcccaggt ggcaggcaca cgcaacgtca
     3841 ccttcgccat cgcctacatg ctcacctccc tgattgcagg cgccatcctg aaaaatctac
     3901 cctttgaaac gggctatcaa gtcatctttg cgattggagc attcggcgca gcgatgagca
     3961 gctaccacat ttatcacgtc acacccctgg aagagaccgc cccgctcccc gccataccga
     4021 cacctgcccc aacaccccaa acagactctc cccgcagcct ccacaccacc ctccgcctcg
     4081 acatctggaa caccaaattc cgcaacgtcc tgcttgttct attcttcttt cacctcacac
     4141 acttcctccc cacgcccatc tacccgcttt acaacgtccg catcctcaac ctcaacgaca
     4201 acaacatcgg cattggcact gccatctact acctcaccgt cctcatcggc tccacccaat
     4261 tccgccgcat cgcccaccgc tacggcaaca aaaaggtcac agcttgggga gcggcgggca
     4321 tggccatcta tccgctgtta ctggcttttt caggaaacat atggcaattc tatggcctct
     4381 ccttcatcgg cggcttcctc ttcgccatga tcaacggcgc ctacatcaac tacatgctcg
     4441 aaaacatccc ccccaatgac cgtccctccc accttgcgtg gtacaccatt attctaaaca
     4501 cggccatcct tgcaggctcc ttcctcgccc cagccatcgc cgaaaccatc ggcctcgcca
     4561 acgcccttat cttcatcggc atcctccgcc tcctcgcagg tctctccgtc cacaaatggg
     4621 gctaatctga accttcaacc tgcaaccttc aactttcaac ctgtaacctt cttatgctaa
     4681 aatcaacccg tgacagaaga tatcaaagta atcgcaacca accgtaaagc cagcttcgaa
     4741 tacttcctga tggaaaagtt tgaagcaggc attgtcctgc aaggctccga gatcaaatcc
     4801 gtccgcgccg ggcaggtcag catccaggaa gcctacgtcg acatttcaga tggggaacaa
     4861 gcctggcttg tcgaagcgca catcgctccc tacgaacagg caggcaaata cttcaaccac
     4921 gaaccgaaac gcaaacgccg cctgctcctg cataaaaaac agatccgcga actgtggaac
     4981 aacgtccgca tcaaaggcaa caccgttgtc cccacccgcg tctacctcaa agacggacgt
     5041 gcaaagatcg agatcgccct tgccaaaggt aaaaaagcat acgataaacg tgccaccatc
     5101 gccaaacgcg atgaagcccg cagcaacgaa cgcgcgacca gagtgagata gttgtcggta
     5161 tctggactta tcgtctatcg cataggctat aagaccaaaa gaccagcaac aaggagacta
     5221 caccatgccc ccctccacca tcggcggaat caacctgttg cctgaatcag aaaagcgggc
     5281 catctattca cggtacatcc caaccgtact tctggaaaaa tacaacctcc ctccgctcac
     5341 ctcagcggca ggctacaacc ttttacaatt ccgctttgcc acgggctcaa cagatgtgga
     5401 aatgcgcctt tttcatcagg tggattttcc cgatcctatt ttatacgccc acctcaccga
     5461 caccctcaat gggcagatcc atgttttgct ttacattctc aacgatcctg catcgcctcg
     5521 cttcaatgta gacagaatgc cagatggctc cccgaccaaa ttcggcaccc tgcaaaggaa
     5581 tatcccatca gaaacagaag ccctcgaagc aggtctggcc ccaggccagg tgcggcgcgg
     5641 attaaggctg ttaggtcagg ccatcgaagc cttcgaagga tttgttgcgg cccttggtca
     5701 tgatatgtat tttgttgaac ctttgtatta tcacaacgcg gtcatcttcg aacgttacgg
     5761 tttttcatat caagttggca aacgcctcat ggacagcatc cacgcagggt ttcaaaatgg
     5821 aggcgatctc gccgccaaac tcgacggttc cacccccttc cgccaccctg aagccgcaaa
     5881 cgggattcgc aaacgctcct gggccattca tgatggtctg cttgatgagc ctttcaccaa
     5941 tgtaaccatg tacaaacgtg ttggcaagtc agctggcatc aacaccacac cggactgtaa
     6001 atggtaggaa taaaataaaa agagaccgtg taaaacggtc tctttttatt tagtagcggg
     6061 ggcaggattt gaacctgcga ccttcgggtt atgcatacca cttcgacttt cgccgcctga
     6121 ccattgtcag tttgtggtct ggactttctc ttcacccaca gcagtatctg tttgggtgcc
     6181 tgccattaag tctctacacc ttccctgaat attcttcagg gcttggctcg ggattacctt
     6241 tgcgaaaggc ttccccgaat ttgacaagtt atcacgaatg agtttcctca ttcgcagccc
     6301 attgctggat tagtttccaa gcatcacgat attcaaaaga cctcggtctt ctttgacgct
     6361 tgtcagtttc ggtcatatgt atttcgctac cgtaactgat gaacacgttt acaggaaata
     6421 tgtaaaacaa actgcaatcc ggcaaataca caagggcaaa atcaaagtct gtttcagtgt
     6481 attcatctct aatcatggac cgcctatttg tcttcgttct acggttatca actacataat
     6541 tttgagatgg aacatcaaac caagcacatt tgacttgcac cttaatcagc acgccgaaaa
     6601 catcaaaaac caaatcgtag ggcagattat caccaactgg gtgaagaacg ccccaacctc
     6661 gcttcaatgc ttctagaact gctgcctgct ctgcaatatc tcctttatgt ttcgtattca
     6721 tttgttaaac ttctcccgcg gtcgagcccg acgagctgcc actgctccac cccgcatcgt
     6781 ttagtaaagc ccgccgatat taccaacaaa cgatacaaaa gtcaagcaat gcaatcaagc
     6841 tgctcattac aaaatattta tgcaactctc atcaaattac tatgaagcgg tacttttgga
     6901 taaaaacaat ttgttaagat ttagtgtata ataacgaaac cttaacaaaa tcactgcaaa
     6961 cattttacat gattgcgtta agtattccaa cagaaacaca ggcatcattc actcattcat
     7021 tatgaagacc acaacgcttc tggtcataga agttcaccat tcagagatcc cttcctttgc
     7081 acctgatttg caaaagaagg gatttgatgt acaaattgca ccaaatggca gcaaagcagt
     7141 ggctcgtctt aaagatgtca gcccaagcct tgtggtgatc aacgcagcat ctttgcgcag
     7201 cacgggcttg cgcatctgtc aggcgattcg cgaaaaagat gcaaagctcc ccattatctt
     7261 aattgttgaa aatgacggtg atgtcagcaa agatgcagcc gatgcggtgt tgggactgcc
     7321 tttcaccgtg cagaaacttg ttaatcgtat taaaccgctc ctgcctggcg atggaaaaaa
     7381 tgttttgcat gtcggcccca ttcgtcttga tttggaaaaa cgccgcgttc gttgcctcgg
     7441 taaaagcaca aaactcaccc cacggcttgt gactttgctc caccttctca tggataaaca
     7501 tggcgaagtg gttgaacggg aacccctttt caaaaaagca tgggaaacga attacaccgg
     7561 cgacacacgc actctggatg tgcatatctc atggttgcgt cgcgccattg aacttgaccc
     7621 cgacaatcca aaattcctca aaacgattcg cggtgttggc tatcgactag atatataact
     7681 ggtattgtgt tccctgaacc gttaccaatc ggatacaagc attgtgcttg ggtccgattt
     7741 ttatttccaa ctcaaggggg atttcgccgc ttcgtgcatg acggtgagcc tgcactgcgc
     7801 ttttgaaggt ttttccatcc caatcaggat ccacctccca aaaatccaca tcgagagacg
     7861 tctccaaacg aatcgaattt tccagcacat gtgccttgac ttttttagca ccgtccaaaa
     7921 aaacgctttt gatggattcg tctcggtcca ccgtaaaaac cgattttgct tccacaagcc
     7981 tgcctcgcac agtattcaca gcacggacag cttcatcact ggtaatgaac tttcgtccat
     8041 gcttcgcagc caccaaagct gttgtgcctg aaccacagaa aaaatcggca accaaatcac
     8101 ctttgttgga cgaagcaagg ataatccgct caagtaacgc ttcgggtttt tgagttgggt
     8161 agcctgtgcg ctcattgtgc aaacgcgcca ctaccggaaa gtaccaccaa tcctcgggaa
     8221 ctttgccgcg ctccaaatta ggaactttgc caaaccccgc cttccgcgat gacttgaagg
     8281 tgttgatggt gctgggatta tatggctcac gaacagcatc tacattaaac gtataatcgc
     8341 cgctttttgc ataagaaagg atggtatcgt gttttcgatt aaaggccgtt ttgatcgggg
     8401 atggtccgtg ataggcccag atgatctcgt tgataaagac ccccgtggac ccaaataatt
     8461 catcgagaat gagacgggca taagcatccg cgtgccaatc gagatgcagg tacaaggtac
     8521 ctttcggaga aagcagacga accatcaatg ccaggcgttc gtataaaaac tgcaaatacg
     8581 aatcgaggtc aggccaggca tcgtggtaac cttcagcgag tttccacttg tgaggcttgc
     8641 gtgagtcttc gccgcggcca atgcgtgcag aaaactttct attggtaaaa aacggcgggt
     8701 cagcataaat caggtcaatg cggccttcat attcaggcag taaggctgcc ataaccgaaa
     8761 gattttcgcc caaaataaca cgccctgacg ggcgtgtttt gggatagcca tgtcctttcg
     8821 ggtagagaat ggcatcttga atcagagaag caggctcagg cgcagcggga tgctttccct
     8881 tccagttgag aataggcata ttgagaaatt agataatgag gcgaggattt tgtttgagag
     8941 gagaaagtcc acaaaaaact agtagtggat ctccaccata gtaccgcgac cgcgatgatt
     9001 ggagatggcc tcggggctgt gattgggctg tacccagcga aagatccagt tggcttcgtt
     9061 tgagcgcatg ttgatacagc catggctcat ctcctgaccg aagttttcat gccaataggt
     9121 gccatggaat gcatgaccaa ccggggtaaa aaacgttgac catgggatgc caggaagaac
     9181 gtaaccactt tcgtcggtgg tgacatcacc acccttgcca atgctgaaat aactgtagcc
     9241 catatgcttg gcgggcacct tgtctaaaac ggtgaatttg ccattggggg tcgttgtgga
     9301 aataccttta tcaccgccca aaccaccacc gggaataccc gatgagatat tggtctgaaa
     9361 aacggatttt ccatattcat acgcaaccag agtttgcgtg gaaagattta cctcgatgag
     9421 tttttcttca taaggaacat tgggcgagat cggctcaaag taactggaag gtaaaactcg
     9481 catatgtatc gcagggacat aatacggcac attcgagtct aactcgtcgt aaatgcgata
     9541 ccatgcgcct gtgtaatcag ccatcttggg gccttcttcc acgccatcca cccaatgcac
     9601 cgacccataa taaataggcg ggttgagagc ctcccaaccg cgtgcctttg taaaacgata
     9661 cggggttgtg tatggaacac tggcttctgt gagtacgcgt ttcccttcag gaacgcttga
     9721 catcggaacc tgataaacgg tctttacgcg atgtaaacgt ccgcgatgca aatacccacc
     9781 ccacacccga taccaaacag gattatgctt tggttcttca gcggtcactt cctcatagac
     9841 atgcacaagg tcatcgcggt accaggtctg gtaaatcctg cttccatctt tgggctcgga
     9901 gcgtactggc atttggtctg tggcaatgcg cacgacaaaa ccatcatcga aatcggtcaa
     9961 tccaggcaaa aagggggtaa aagccaaccc acccagagtc agccctccaa gttttaagaa
    10021 gtcgcgtcgg gaaagttgtg atttcataag gaaaattgta caccaaaaag tttaagattt
    10081 aatgagaatt ccctaaggct gggtaatttt ctcgggatca tccaaaaaaa ctgccctccg
    10141 ccatggcgga gggcagttgc ttattcaatt ttttaggaag ccaccgattc agtgatcatg
    10201 tcttttagaa ggatctcaag ggccattgag tgaccgcagc gtttgtgagt gatgaaaaat
    10261 tcgcaatcac aattaaacac gccattgtta taggatacat gatgctcgct attatcaccg
    10321 tgaaaatcaa gatcaaactg attaaagtga aaacggtgcc tgtcttcagc atatcgtttt
    10381 gctttttcaa ccttgcctat cagtccagaa tccatttttc gtatctcctt tttttgagaa
    10441 tataaaaaaa cacacgcggg aatatccgcg tgtgtttaat caacttgcct gggtatttcc
    10501 atgaaataag cgcatggtgg cgttacctcg atgaagtgag tatagtactt ttcaaaaagc
    10561 aagtcaatga caaatgtcag tttgtggttg attcgtatgg catttgtagg gttttggatt
    10621 tatgtgtcgc tgcgaattac tagactacac gcagactaag ggccgattct aacttaaccg
    10681 acaaatgcct ctacttaatc aaccttttct cacccaaact ctgactaaaa tcacaaaaaa
    10741 ggaagcctca caataatttt atgaggcttc cttaatcagt tacttcgttt ttgtagaact
    10801 cttttttctg acaggcttgc tggttttgac agaagccact ggctttttag attcaactcc
    10861 ctttttgggt ttggttgtct ttttcacagc ctttttactg gttaagacaa tcttctttgc
    10921 ttttgccgcg ggttttgttt caacagcctt cttagctttc ggtgcaactt tctttttggc
    10981 gggctttttt actgtcgcct tctttttcac tggggatttc ttcgctttcg ccagagccgt
    11041 ttcttttttc ttcgttactg ccttcttgac cacacgtttc tttttcgcgg ttttttccac
    11101 aactgacgtc tcttctttgg cggcaaacat ttccttccac ttatcctgca tggaggggac
    11161 gcctgcgccg ttcaactggt cgcaaatggt cttccattca acatcgccta cgaagcgaaa
    11221 atcaacaaag gcgtggcatt gataggcacc aagttcgatg tacatgcctt tttcccagat
    11281 atctttgcag gagcgaatgt attcaagctg ggaggaacaa tctttgaaaa tgacgtaccc
    11341 tgcacgcggc aggctgaggc cttcggcaag attcttgcgc aaggttctgc cagaagattt
    11401 atcaatgtac gcagcggagg ttttgatcca gccgctggtg ttggcaaagc gattgtggta
    11461 gacgatgagt ccgcgttcgt cattgtgacg gttggagtat gcaaacacat cttcatttac
    11521 gttgccgctc gatgtgtaaa agtcaaagag cagaaacttt tccacatcag cgaacaggtg
    11581 tcggcggtga agaagcggga agatccgcca ttcgtgacca cggagaaggc cttcgtcggg
    11641 ggtttcatcc agcttggcgc ggcgatattc cattccgtat ttttcagcaa agccttccac
    11701 ctgcccatgc ccaaacatgg gcaacccagg caacgtggaa agaaccgtgc aaatgccaaa
    11761 gtatttgtca ccgctgccga attgctcaac agccgtcttc tcatcggggt tgttcataaa
    11821 attgacataa cgcttgagga tctgcggatc gaactcaagg gtatttttga tggcaaggcg
    11881 gtatttggca ttgtcttcgt cgcgcagcat gtgcataaaa gcagagttgt aaacacggtg
    11941 catgccaagc gtgcgcacaa aatacccctc catcaaccag aaagcctctg caagcaaaag
    12001 cgtatctgga acttcagcag cgacacggtc caccacttca cgccagaatt cctctggaac
    12061 cttttcgtca aattcagatt tggtcatacc gtattgtgag cgtgatggaa tcgccccacc
    12121 cgccccaggt tcgggaaacc acaggcgctg aacatgtttc ttggcaaggg tcatggccgc
    12181 atcgaaacga atgacgggga aattgcgagc cacatgcaaa atgacttgaa tcaccgcttc
    12241 acggacgacg gggttggtgt agtccaattg cgctgtatcg ttccacggga aggacgtgcc
    12301 atcgttgcca tgataaatgt agcgcaaatc acctgtttgc aggtcgcggc gctgaaatac
    12361 cactgcggca tcggtcttgt cgtaataatg gtcttcaagg taaatgccaa ctcgcaggtc
    12421 gttggagagg ttttcagagc ggaaagaata agatggatac ggcgggttcg atgtggagag
    12481 gaaccactcg gggtgttcca ccacccaggt tgagtcgatc cccatgtgat tgggaaccat
    12541 gtcggcggaa agacggatgc cgcgtgccca tgcacgagag cgaaggttgt tcaaggcatt
    12601 ccagccgccg aggtcacttg caatatcgta ggagtgaagg gagtatgcgg aggcaactgc
    12661 atcggcctgc cccatacgtt gtttgatgat ctgggaagcg cggctgcgtt cccaaaggcc
    12721 gatcaaccaa aggcctgtga atccacgtga ggcgagcaaa tccagttctt catcaggaat
    12781 ttgatcgaga gcggaaattt cgcgttgata ttttttcgaa agctggccaa gccagacata
    12841 cgaatttttt gcgagcaaaa caaggcgggg catccaatct ttgtcggggc tgtagcgttc
    12901 atattcagca tattcactgc cgacaaaact gggaacgtag gtctccacct tttgcgaggg
    12961 atcgatctgt ctggtttgca gacggatggt ttcttcacgc aggaaatcca tgccgcgcag
    13021 taattttgca gaaaacgcct cgccaagcaa atgtccccac ttttgaataa taaattgtaa
    13081 ttggccttca agcgaatcag gtgacgcttt gatgggcgca agcagtaatt cgttgagggt
    13141 ttctcccaag gcaaattcac cattgccaaa accaggctgg cgggcaaaaa agtcaagcaa
    13201 gagttgcacc agttccttgt acgccgaacc gttcatcacg gattcgtcaa tcaacacttt
    13261 ataacgggat agtgcagggt tgttattgga gttattgatc agcagcattt cttccacggc
    13321 ggcagcgcgc acgccatgac caaaatcgcc aaaattcgga agcttggtgg caagatacac
    13381 acccgccgtt gtctgcccta aaaagacaga caggggtgga aattcttcga taaatttact
    13441 gagggtcaag tcgtattttg agccaaggtt cgattgcaaa acccccatgg cacggcccat
    13501 cacgccggtg tatctgctga aataatgttt gaggatgatg tggtaggcct catccagcag
    13561 gtagaatgca tataggtcag aaccaggtac cgggtcggtt cgaggcgttg acatttgggc
    13621 ggcaaaggct cgggcggcgg tcaaatccgc gaaggttacg tgtccgtccg ggcgaaagaa
    13681 atccacagaa aaattgaatt tgagacggga tgcgcgggaa attagcataa caacaatgat
    13741 aaccgattat cgaatttttt cgggtaaaat cctttctgca agcatgaaca catcacccac
    13801 actttttgtc ttattgctcc tgctgggggc cttatacctg cccatcattt tcttcgccat
    13861 ccaaaggcgt gatgacggcc acggcatctc catttggctg gcggcttttt ttgcgcttct
    13921 tgccatggtg ttgaatgttg ctgaagccat tgaagcctca caaggcggta cacttctttt
    13981 tcaggaagtg cagatctata ttgcgttcac attgatcgca atcctcatgc tggcatttca
    14041 aaatttcatg cggtatgaga cctggtgggt ttgggttggt gtgtggacgt tatggatgct
    14101 tggccttgcg ctgattttga ccaatgcact caagctgccc gatgtgttgt ggagcaatgg
    14161 cacactcacc ctgccccgtg aacggcttgg accctcctgg gcaattttag ggtggttgat
    14221 catttcgatc gcgctggtaa tctcgatctc aaacgcaggc aagcaggcgc gccagcaact
    14281 gttccgcaac cgtctgaact attggtggcc tactgttttc ctgttgttcg tcaatgacgt
    14341 tctccttttt tccaatatca tgattgcagg ccagcccctg cgcctgatgg cagcggtgtt
    14401 gatggtgtac gtcatcggca ctcataacgt acccgacctg cgccagatct tccgtcgcat
    14461 tctggcttat gtggccattg ccgtggtcat tgtcggcgcg tatgcggcag gctacctctt
    14521 gattgaagct gtttttggaa attcgaaaaa cttcaatccg ctttttgcag gcgctttcat
    14581 tgctctgttg ttgaccttga tctttgcacc aatgttgaac ctggtttcac gcgcggtaaa
    14641 caattgggtc aagggcgatc aatacaaccc cagccatacc attcataaat acagcgaaag
    14701 catcagcaat attttggaca tggaccgcct tgccagcatt gccgtgggca tcattctgga
    14761 agccatgcaa atcgagcgcg gatttttatt tttggtggac tcagaccgcg aagcagatgg
    14821 gcatagaacc tacagacttc gctcagcccg cagccctgaa gaacgtcaaa tgatcgcagt
    14881 ggaattggat gaaaacggaa tcatcgcctc gtacttcatc cgcgaacaaa aaccgcttct
    14941 tcaatatgac ctggacctgc ttcccgcgtt tcgggctgct tcaccctttg aaagagactg
    15001 gttcaaccag ctccacactg aagtgtatat tccgatcttt gccaagaagc aatggatcgg
    15061 cctgctggcc tttggcccca aactcagccg aaaccgctac accagtgaag acctcgtcac
    15121 tctcagttca cacgccaacc agaccgccat cgcacttgaa aatgccagac tggttgataa
    15181 cctcatgcgg ctgaataacg aactgcgtca ggcacgccgc gctctcgaaa aaaataatcg
    15241 cgatctggaa cgcatcgacc aggctaaatc cgatttcatc agtatcgcct cgcacgaact
    15301 gcgcacccct ttgaccgtca tcaaaggcta tactgaaatg ctgctcgaag ataccagcct
    15361 cgaccccaat tacaagcaga tcatgaacag catccacgac ggcaccatcc gcctgcacga
    15421 agtcatggac tcgatgttcg acatcgcaca gatcgatgcg cgttccctaa aaccacacat
    15481 ccagcccgtt gaaatagggc atttgctcca ggaagtatgt atcgagcagg cttcctccat
    15541 caaagagcgg aagcactccc tcagcattga cctgcccagt ctgccccacc tgaaatgcga
    15601 ccccaacctg atgcgaaaat tgttccatca cttgattcgt aatgcggtca aattcacacc
    15661 caacaatggc aagatcaccg tcaccggcca cagcatcccc gcaatcattg acctgcccaa
    15721 tggcggtctt gaaatcgtca tcgctgatac gggtgtcggt gtagatccaa atttccgtga
    15781 aatcatcttc acgaaattct accaaccggg cgaactgggc aaacactcca ccagcaaaac
    15841 acgttttaag ggcggcggcg ccggactagg ccttgccctt gcaaaaggca ttgtcgaagc
    15901 gcacggcgga cgtatttatg tggaaagccc cggttacgat gaaatcaact tccccggcag
    15961 tcaatttcat gtaatccttc cactgaccaa acccgaagaa ggcgaagaac aaaaaacaag
    16021 cacccctgtt caatttcaag tgtaaataaa aatggcttgg caaacatatc caggcctttt
    16081 ttgttccaac cctcctgaca gacaggcaat aaatcggatt ggttaaatat gaaatagaaa
    16141 gtatttttag gggcaaaata agcacgtcca tgctatactt gcaaaaaatc cttctaccga
    16201 gaccccatgc ctggaaaatc ccaccaagag ctcttaaaac aatacgctga agcgatcgta
    16261 aaagtaggtt taaatattcg cgctggccaa cgtttgatca tcaccctcgc cgctacacgt
    16321 ggtgttccct atcaatttgc acccctggtt cacgaagtta ccaaggccgc atacgccgca
    16381 ggtgcaaaat atgtggaagt aatatggagt gatgaagaaa tgatgcgtct gcgggcagaa
    16441 cacgccccca aagactcctt tagcgaatac ccaacctggc aagtggatgc gatcttgaaa
    16501 atgatcgaaa atggagatgc actcctctcc atcggcggct caaatccaga tttgcttggc
    16561 agccttgacc ctgaaaaggt cggggccatg caaagaaccc atttgcagta ttacagcaag
    16621 gtcagcgaaa aggtcaccac caatgcaatc aactggtgtg ttgtggctgc tgccggtgaa
    16681 acttgggcaa agaagatctt tccagatctg gatgcacaaa cagcacagca aaaattatgg
    16741 gatgcaatct tcgaaaccac ccgcattgac cagcctgacc ccattgccgc ctgggaaacc
    16801 catatcgtca atctgcgcaa acgcgccaaa tatctgcaag ccaaacaata cacaggcctg
    16861 cactattccg cccctggcac cgatttcaca cttggccttc ccagtggtca ccaatggatc
    16921 agcgcacaat ccatggcgca aaacggcgtc atgttcaccg ccaacatgcc gaccgaagag
    16981 gtgtttactc tgcccgatag aaatcgcgca gatggtattg tcacatccac cttcccgcta
    17041 agttacggcg gcaccttgat cgaagatttt caggtaactt tcgaaaaagg ccgtatcgtc
    17101 aaagtcagtg caagaaaagg cgaagcagcc ctgcaaagac tggtggatac cgatgaaggc
    17161 tcgcaccgcc tcggcgaagt cgcccttgtc cctgccagct cccccatcgc caaacgcgga
    17221 catctcttct acaacacctt gtacgacgag aatgcctcct gtcatatcgc aatcggacga
    17281 gcataccgct tcactcttac gggtggagag gaactgaccg acgaggaatt tgtcagcgcc
    17341 ggtggcaatg tcagcctcaa ccatgttgac tttatgatcg gttctccaaa gatggacatt
    17401 gacggtatca aaaaggacgg ttctcgcgaa ccagtcatgc gtcagggtga atgggcaatt
    17461 gaagcgtaaa acggacctcc cgccgaaatc aagatcggca ggaggtttta tctttaaaaa
    17521 caaaaggtga atcatggacg atcaggaatt taatcaactg ctcgaaaaat acgccgaagt
    17581 tgtcgtaaaa gttggcttga atttgaggaa cgaccagcac ttatccattc gcggaaatgt
    17641 cgatgacgcc ccatttatca ggaaggtcgc cgaaaccgca tacaaggccg gggcaagata
    17701 tgtggacgtt ttatgggatg atgaaaatat caaacgcacc cgcttgcagc acgccgaccc
    17761 tgaaaccctc accaccctgc ccgattggct ctataaccgt tacgaagaat acagcaaacg
    17821 cggagatgcc gaactcgcca ttctctccag caacccagac ctatacaatg gaattgactc
    17881 cgaccgaatt gcaattacaa gaaaaaccct ttatcaaaaa atcgtcgagc cgctacgcaa
    17941 atacaacaac tacgccaact ggtgtgtagt ttccacggct gcacccgcct gggcacaaaa
    18001 ggtctttccc aacctgcctg aaaaagaagc ccaaacaaaa ttatgggatg caatctttcg
    18061 cgcctgccgc atccacaccc ccgacccggt aaaagcctgg gaagagcaca ttcaaaaact
    18121 ggataaatac aaaaactatc taaacgcaaa acgatttgcc agcctgcgtt acaaagctcc
    18181 cggcacagac ctaacgatcg gcctgcctga agaccatcac tggggcggcg cacaggaaac
    18241 cttcaagaat ggcattaccg gcacggtaaa cattcccaca gaggaagtat tcaccatgcc
    18301 tcacaaaaat aaagtggacg gtctggtaac agccacactc ccactgaaca atcagggtgt
    18361 tttgatggaa gaattcagca tccgctttga aaacggacgt gcggtcaatt tttctgcaaa
    18421 aaaaggagat gccgaccttc gcaaactcat cgaaaccgac gaaaacgcgg gtcgtcttgg
    18481 cgaggtggca ctggtaccca acagctcccc catcagccaa agcggaatcc tgttttacaa
    18541 cacccttttt gatgaaaacg catcctgcca tcttgccctt ggcagcgcct acaaatcatc
    18601 catgcagggc ggcgtggaca tgacagatga agaatttgca gcacgcggtg gaaacaaaag
    18661 cctgatccat gtggatttca tgatcggctc tgccaaaatg gatattgacg gaattcaagc
    18721 tgacggaaca cgcgaacccc tgatgcgcaa cggtgaatgg gcctttagcg tataagaaca
    18781 tgacaacaaa caaacttgcc catacaatgg aggctacatg aatactgctg aatttcaaaa
    18841 atcgttagaa aaatatgccg atgtgatcgt caaagtcggg atcaacctgc gcaagggcca
    18901 gaacctgacc atctctgcaa gcatcaccga ttatccgctg gtacgagcca ttacaaaaag
    18961 cgcctatgcc agtggcgcaa aactcgtcac cgttttttat ggagacgacg aggtttttca
    19021 cacccgcatg aaaatgagct cgcacaaaaa tctcacagaa gtcccttcat ggatcgttcc
    19081 cgcctacaca agcattgtca agaacggaga tgcctacatc ggcatcacct ccgataaccc
    19141 agacctgctt gcagatgtag acccggaagc cattgccatt taccgcaaaa ccatgggtac
    19201 aaaaatgcgc aaactcaccc aagctgtgat gcgcgacgcc atcaactggt gtgtgatctc
    19261 ctaccccgct gaaaattggg caaaaaaggt cttccccaaa acctccacaa aacaagctca
    19321 ggaaaaatta tgggaagcta ttttcaaaac ctgccgtgta gaccagcgcg acccggttgc
    19381 cgcctgggaa aaacatatta aagacttaag ccttcgaagt gcctatttga acaaaaaacg
    19441 atatgcagcc ctgcactaca aagcccccgg caccgacctg accgttggcc tgcccagcca
    19501 acataaatgg gtcgcagccc gaagtaaaag ccaggccggt attgacttca ccgccaacct
    19561 tcccaccgaa gaagtattca ccatgcctca caaggataag gtggatggtt ttgtggctgc
    19621 atcacgcccc ctggtcattg caggcacaac tgtggaagat ttcaccctta cctttgaaaa
    19681 aggcagggtt gtaaaagtca ctgcaaaaaa acatgaggaa accctgcgca aattgatcga
    19741 aaccgatgaa ggcgccgcac gcctcgggga agtggctctg gttccaaata gctcccccat
    19801 cagccagagc ggaatcctct tctacaacac cctctttgac gaaaatgcct ccagccatat
    19861 tgcgctggga gccgcctacc ccttcacaat gaaagatggg aacaaattat cgcaaggcga
    19921 tttccaaaaa cgcggtggaa atcacagcat catccacacc gattttatga tcggatcaaa
    19981 ggatatggat attgacggca tcctcgcaaa cgggaaacgc gaacccctca tgcgaaaagg
    20041 tgaatgggcc ttcaaagcct aaccgtctga caccgcactc cacgcccgat ggtcaccgcc
    20101 atcgggcttt ttttatcggc gttataataa aagcatgcct gcaaattccc ccaaaaaaga
    20161 aaccccaatt tcaaaagact cgatggatct tctgatcgca gaatataact atattgctgc
    20221 aacagctgca cagtcgaatg aagaccgtgc ccgtgtgtca tccttttatc tggtcgcggt
    20281 tggttcgttg gtggctgcat tattcagcac acaattccta aacaacgtgt tcaaccctga
    20341 cctgatcaat atcctgttca gcatcctttt tctgctactc accctgttgg gaacaaccac
    20401 cattctgcaa ttgacccgcc tgcgcgtggc ttggttcgaa tcactgatcg ccatggacag
    20461 gatcaaagaa ttcatcatcg ccagagataa atcacttgca ggggcattca ggtggcggat
    20521 tcaaaacgcc cctgaattct tcaaacccaa cagcgtctcc ttcctgcaaa cagtggaagt
    20581 tgccattctc agcgcactca cctttggcac cggggtcttt ttcttccaaa aagggattca
    20641 atacaattgc gcgccatgca attgggcata ctccatcgca ttgggattgc tggcattctt
    20701 ccttcagata tgggtttaca aaaagcaatt agtgctggaa aaaaaacgct tgcaaaagga
    20761 attttcaaac aaccatgact cgtaactacc ttggccaacc gcccaccgca tttcaacgcc
    20821 gcccgcatct aacccgtgac gatgaatgga tcgcggccct tcttaaagtt gcacaagtgg
    20881 ggcatatcgc ctcagcctat gagaaccagc catttatcaa cccaaccaca ttctggttcg
    20941 atgaagaaaa tcaccagatc gtgttccact cgaatattgc ggggcgggtg cgttcaaaca
    21001 tcgaagcaaa tccaaaagta tgcctcgaag tcagtgagct aggaaagtta ctaccctcca
    21061 atgtggcgct tgaattctca cttcaatacc gcagtgcggt cgtatttggt gaggcacgtc
    21121 ttgtcagcga accaaacgag gccaaacaac tgctctatgg cttgatcagt aaatacttcc
    21181 ctaaaatgac cgcaggcaaa gaataccgcg agatcaccga caaggaattg cgcgccactt
    21241 cggtctatgc gatcaaaatc gaatcgtgga gcggaaaaga aaactgggaa gcccgcgccg
    21301 accaatcaga cgaatggcct gcgttggacg agaaatggtt cgagtaaagg agtcccaaca
    21361 tgcccgatga ttttcgcaca gaggaattca aggtagacgg cgaaaaattg atcgccaaga
    21421 tcaaagaatt gatccatgag gggaacatcc gccgtatcat catcaaagac aaggaaggca
    21481 agaccatcat ggagattccg ctgacctttg gtgtggtggg agtattgctt gcacccacac
    21541 tggccgccat cggtgcagtt gcggccttgc tgaccgaggc cacgatcgta gtggaaaaaa
    21601 gcgcgtagag agataaacaa tggcaatttt caaactcgtc ttttgggcag ccatcattgc
    21661 cgaaatggtc attcgagcgc cgatcagcag gcaacaacgg aaagaagcga aatctgagca
    21721 aagagtgtct gcacaggaaa agaccatcct tgggtttttg ttcgtcggaa tgttcctgct
    21781 cccgctgatc tattcgacca cgaactggct ggattttgcc aattactccc tgccgaattg
    21841 ggctggctgg cttggcgtgg ttctgatggg atttgccctg cttattttct ggcgctcaca
    21901 caaagacctg ggattgaact ggtcgccttc gctggaaatc cgcaaggaac acactctcat
    21961 cacaaacggt atctttgggt atatccgcca cccaatgtat gcttcacaat gggtttgggt
    22021 cattgcccag ccgctgctgt tgcaaaactg gctggccggg tttctcaatt tcctgatttt
    22081 tattccgttc tatattttgc gggtccgtgc tgaagagcag atgatgctgg atagatttgg
    22141 cgacgaatat cgaacctata tgcaaaaaac gggcgcgatt tttccgaaag gcaaataagc
    22201 atgaacagag attttcaaat taagaacaaa acctcacgcg aaaaaatgcg tgaactgatc
    22261 gaagggatga ccgacgcaga actggctctg cctgctgtgg caggctggac cgtggccgca
    22321 acctttgcac atgtggcctt ttgggatttt cgaatcctgc acctgttgag gttgtggcgt
    22381 gaaaatgaga tcgtcccctc tccctacgat gtggatgcga tcaatgaagc ccaaaagccg
    22441 ctttgcatgg cacttccacc ccgaactgct gcagagttgg cactgaaagc cgccctggaa
    22501 attgacagcg aaatcgaagc cgcctcggat gatctggtaa aaaagataca ggatgccaat
    22561 gttcagttca agtttgagcg caatggacat cgtgagtatc acatcaaaga aattgagcag
    22621 gtcttggcgg ttcatcgggc aaaataaccc gacgattggc ttgagatttt tgtagattct
    22681 ccctacgggg agaatctttt tatttaacag aatcaggttt caggtcgggt gggaaccgcg
    22741 gatttccttc ccaaaagaat gtgacatcct tccatgccca aatgcagcga ttcctgaaat
    22801 aataaggtca gccataacat ttttgagagg ccaaccttat gaccattcag tatctaagcg
    22861 aaagcacgga aatgtacttg aaagcgatgc ttgaattaag cgatcgtgaa aaagtggccg
    22921 tggggcgttt ggcagaacgt ttaaatgtga cgcctgtttc agcgaatgaa atggtgcgaa
    22981 ggttaagcga gcagggtttg atggcacata caccttacaa gggcgtgtca ttgacggcaa
    23041 agggacgcaa ggcggcttgc aatgtgctgc gccgacaacg tttgtgggaa tgttttttgt
    23101 acgaacattt gaagatcgaa tgggcaaaaa tctatgaact ggcttgttcg cttgaacatg
    23161 ccacagcacc cgaagtgaca gaggcgctgt cagttttttt gggtgacccg aaaacttgcc
    23221 cgcgaggcaa cccgatccct gcagcagatg ggacgtttgc accgctggaa ggtacaccgc
    23281 tcagcatggc acccatcggc gcaacggttg aggtattggc tgtaaatgcc accgccaccg
    23341 atgtgctgac ttatttattt gaacgcggca ttctgccagg taaaaaaata acgatcacag
    23401 aagcggcacc gttgcagggt ccgctgacat tgaaggctgg cggaaaagaa accgcgttgg
    23461 gcctttcaat ggcggagttc gtcatcgtga aaacaaatta acaaaaggaa aacaatcatg
    23521 ccaagctcaa ctcaattgca tttattacag cctgggcagc gagccaccat tgtacaaatc
    23581 aacggagcgg gcgctttgcg gcgtcgtttt gttgaaatgg gtattgtgcg gggggaaacg
    23641 atcctgatcg aacgcaatgc gccgctgggc gaccctgttg aatacttcat caagggctat
    23701 catctggcat tgcgcagaga ggaagccgcg cagatcgaag tcaatctcga cgggggcaat
    23761 tgatggcaaa cctgacaatc gccctggcgg ggaatccaaa tgctggaaag accaccatct
    23821 tcaacctgct gacaggtctg caccagcaca caggtaactg gccaggcaag accgttgaaa
    23881 aaaaagaagg cgagatcgag cacaacggta tgagcatcaa cattgttgac ctgccaggaa
    23941 cgtacagcct cacagcttat tcgccagaag agatcattgc ccgtgatttt attattcaag
    24001 aaaagcccga tgtggtcatc aatgtgatcg acgccaccaa ccttgaacgc aacctctacc
    24061 tcaccgtgca gctgctcgag ctcgacgtgc ccgtggtgct ggcgttgaac atgaccgatg
    24121 ccctgcaaaa gaacggcaca aaaatcaaca cagagcacct ctcgcaaatg ctgggcggca
    24181 ttcccattct gttcacagcg gcaaacaaag gcaggggcat ttcagacttg atgaccaaag
    24241 caattgaatc tgcacgcaaa ataaaagaga ccgcataatg caccctacac cgcaaaccat
    24301 ttttcgcctt gattacggcg aaacactcga aaccgagatc gcgagcatcc tggcgatctt
    24361 tgaaagcatg aaattcgata cggggaaata ttccaaacgc tggcttgcca tcaaacttct
    24421 tgaaaaagat tcagatatcc tcgaacatat ccaatccatg caaggcggtc gtgaagtcat
    24481 tcaatttgcc gttgaaagag caaaacaggt tgaatccaca ttgggcgaag aaattgacct
    24541 gctcaccgcc gaccgccgct atggcttcat caatggcatc gtccgcaaat cgctacaacg
    24601 tccgctcatt gaccgcatca ccctcaccga ccgtatcgac gacatcgtga cacacaaatg
    24661 gctggggctg ccatttttct tcgccatcat gtatgtgatg tttcgattgg tgatcgacgt
    24721 atcctctccg tttttggatt ggacggacac cctcatcaac ggtccgatcg cccatctttt
    24781 ctcgttcctg ctggacctcg tacaggctcc cgcgtggatg cattctctcc tcattgacgg
    24841 gatcgtggca ggcgtgggcg gtgtgctggt ctttgtcccc gggctgttga ttctgtactt
    24901 cttcctggcc ctgctggaag attcggggta catgtcgcgg gccgcttttg tgatggaccg
    24961 atttatgcgt gtggtgggcc tgcacggcaa atcttttatc ccgatgatcc ttgggtttgg
    25021 gtgtgccgtc cctgcggtgt atgccactcg gacaattgcc agccgtcggg ataggatctt
    25081 gaccgcgctc ctcgtcccgc tgatgtcttg ctcggcacgc cttcctgtct acgttgtatt
    25141 tggtctggca ttctttggca gtaaagcagg gacagtgatc tgggcaatgt atgcgcttgg
    25201 aatccttgtg gccatgctgg ctggcatggt tttcacccgc accctgctaa agcctgattc
    25261 cacttccgcc tttgtgctcg aactgccgcc ctatcgccag cctgcaatga aaagcattct
    25321 cattcacatg tgggagaaca cgcgtgaatt tgtccgcaag gcgggcacgg tcattcttgg
    25381 ggcatcggtc atcatgtggt tgttattgaa ccttccgctg ggagtgacca atcaaaaaga
    25441 ttcctatttc ggaaaggtca gtggattcat tgccccgatc ttttcaccgc ttggttttgg
    25501 caactggcaa accagcggcg ccttggtgac agggtttgcc gcgaaggaaa tcgtggtcag
    25561 caccatgagt ttggtttaca caggcagcga aaccacggcc aacgctgaac ctgccacgtt
    25621 ccgcgaggag atcatcagca ccggcaaggg atttgtggaa gccaccatca attctggcaa
    25681 aattctagtc agcattctgc caggcataga cctgattcct gaagatgagc aaaccgaaga
    25741 tactgttctc agcattgccc tgcgcaacga atttacgccg ctgagcgcgg ctgccttgct
    25801 ggtctttgtc ctgttatatg taccgtgtgt ggcaacgtta ggggcgatca aacatgaatt
    25861 tggtaattca tgggcggtaa cctctgccct gtatcaaaca gcaattgcct ggtgcacggc
    25921 cttccttgtt tatcaaggcg ggaaaatact tggctttgga taactcaatg ttaggccaat
    25981 tattacagct tctggaagat aaagaaaacg gtctaagcct ttcagaaatc agccgcgcct
    26041 tgaacgccca acccactgcc gtgttagcca tgatcgcata tcttgtgcag aagggaaaac
    26101 tgctggaaat cggcccggac ggaggtcatt gtgccagttg tgggttggtc tctgattgca
    26161 acctgcttgc tgcgcgtgga aaacgatacc tccgcgctga attgagagat gggaaaagca
    26221 tccccggcta cgtggcacct attgagtagc agaagataac accgtttact gacaacaaca
    26281 gcagggttga gatgatatct caaccctgct gttgtctgcc acaaaattat gatcgtttcg
    26341 aatataaatc tttattcatt cgccaaagga atgcgaagtg aaaccttcaa gccatcgcgg
    26401 tcttcgcggt tggtgatcac cacttctcca cccacctgcc aaatgatcga ggtaattgtc
    26461 gggatgccca gacccattcc cggggtttcg ccagtgtaga atttttcacc ttgaaaataa
    26521 ggcatacgtg cccatttcaa ttgttccgcc gtcaggcgaa ttccatcatc catcacggtc
    26581 accctgacca tgttctcatg ttccacaggc tcaacgagaa catccacctg cggagcgtga
    26641 tctgggtgga acttcttgct attttcgatc aattctccca gaaccaactc gaaggcataa
    26701 tctgtaattc cgaggcaggc agtcatcagg tcaggatgaa tcgtcaagtt gatgttgcca
    26761 agtccctgtt cgttttggat cgatttcaac atttcgggca ggaatcgaat ttcaagcgga
    26821 atattactct ccatcgccag tggtgtatcg atgtaatcca aaaccttgtt cacatcaccc
    26881 gccaggaaat taatagcctc cagagacagg gatacaaatc ctttgatctc ggattcagaa
    26941 agtttttgaa gcctgttctc gagcatgctc atgctcatca caagcgtgga gaatggcgag
    27001 cggagtttat gcgtcaccgc tttatggaat ttccgcatgt cgtgatgtgt ggaaagcaat
    27061 tcggtcacat cgcgcacacg aacaacgcgg ctgttgtcac tcttcaccgg catatcgatc
    27121 gcttccagca acagccaaaa ggcccgcgca ttggcggttt caggctgaac aagataacac
    27181 ggcgccgggg tatcgagcca ggcagtccag gtttccgttg ggtagggtga ataatgtttt
    27241 acaacatggc tggtgatatt gatcccaacg cactcacggg gaaggtgtaa aaaagtctgt
    27301 gcgcttgagt tggcatattg aatggttcca tccgaactaa gcagcagata gccttcgtag
    27361 ctgttttcaa cgatccatga aaagcgggcc cgctcagcca tcaaacgccg atatcgattg
    27421 atgcgggtga tggtcttcaa gcgcaaatgc agttcaagcc tgtcgaacgg cttggtgatg
    27481 aaatcatcca cgccagccat gaatccggtc aggcgggcat cgcgatcatc cagcgaggtc
    27541 agcatgacga tatgtacctc actcaggctg gggtctgcgc gaagcgcctg acagacatca
    27601 tagccattca tgcggggcat catcacatcc aaaaggatgg catccggcag gagtttttgc
    27661 gctgtttcaa ttccgcttgg tccatcttct gcgaacgcca ggcgaacgtc atcccctgcc
    27721 aaaagggtct ttatggtctg cctgacaatc ggatcatcat caatgactag gatggttggt
    27781 agttgattgg tcatttttcc ctttgttcaa ttattttcaa atgtaaggct cagatgtaac
    27841 aagaccggca ttgatggcga actttaccag gtctgcatgt gaattgatgc gcaacttttt
    27901 cattgcatta aaacggtgcg actccaccgt ccgtacactt aagattagtt tatcagcaat
    27961 cacagcattg gtctttcctt cgccaacaag ttgtaatatt tccctttcac ggggtgacag
    28021 caggttcagt acatcgcttt ctttttcatt ccccgtctgc aggagcattt cgagcacctc
    28081 ttcggcgatc tccgggctga gataccttct cgaatccatg acagtatcaa tggcacaaag
    28141 aatctcagaa aaatccgcct ccttcaacac ataccccaag gcgccgccct gcaaagcacg
    28201 gacggcataa gcggcattgg caaacatgct cagaaccaga atttttgtct tgaacccgcg
    28261 ttgacgggcg aggcgtgtaa cttcaatgcc attcagaccg ggcatcatta catccaaaat
    28321 ggctacatca ggggaaaacc gctcgatcat ttcaagccca ccctgtccat cgcccgcctc
    28381 accaaccaca gaataacctt tccgagtctc caacaaagaa cgcattccct gccgtatcaa
    28441 tttatgatca tccactagaa ggatcgtaac tggatgcata accgctccca agaaatagtg
    28501 tttttttcgc cagaacgttt cggggaaagg caaagttttt cgatagccta tctaccatta
    28561 catactggaa ctgcagatat attatctaaa caatccaatg attaccatag gtattattac
    28621 ttaatttttc accttcttcc cgctgattta tggttccaaa aggaggttga ccagtagtct
    28681 gcttttggaa ttaagtataa ctatgtaaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28801 nnnnnnnnnn aaacacgaga atcttatgaa atcctaccgc aaagaactat ggttcaacat
    28861 ccctgcacgc cgcggatttg ttaatatcac tgcacaagtg gaagcctgcc ttgaagaaag
    28921 cggcattcag gagggtctta ttttgatcaa cgccatgcac ataactgcct cggttttcat
    28981 caacgatgac gaatcagggc tgcatcaaga ttatgacaag tggctggaaa aactcgctcc
    29041 gcatgaacct gtcagcggat atcgtcacaa cgacacaggt gaagacaatg ccgacgcgca
    29101 tatgaaacgt caggtcatgg gacgtgaagt tgtggtggct gtaacagagg ctaaattgga
    29161 ttttggcccg tgggagcaga tcttttacgg tgaatttgat ggccgccgcc gcaaacgcgt
    29221 gctggtaaaa atcatcggcg aataaagcct caacgtctat tcaaatattt atcaatactt
    29281 aacaaccgag gagatttcaa catgcgttta caaaacaaac tgattgccat attgatcgct
    29341 gaaggcgtgg aagatctgga atattacgtt ccgctcatgc gtctgcagga ggaaggcgca
    29401 aaggtcattt ccgctggtct ggatatgaaa tccgtgcacg gaaaaaacgg tctggtcatt
    29461 acccccgatg cgacaatcga atcgctcaat gctgacgaac tgtttgcaat cattctcccc
    29521 ggaggctggg cgcccgataa aatccgcagg tacgacgtgg tgaaaaatct ggttagaaaa
    29581 ataaatgatg ccgaaaagat catcggcatc atctgtcatg gagggtcgat cgccatttct
    29641 gctggcatca tcaaaaaagg tcagcgcgcc acggggtcaa cgggcatcaa ggatgacctt
    29701 gtcaacgcgg gtgcggtttg ggctgatgaa gctgcctttc gtgagggcaa tcaggtatgg
    29761 ggtcgggtgg ttgcggacat tcccgatttc aaccgcgaac ttgtaaaggc gctagtcgag
    29821 ggatagcggc ggatatttca tcgactcagg gattgggacg ttcagtaact tgagaatcgt
    29881 aggggcgatc tgcaaatgag aaatgacctc acccgtctcg cctcttcccc tacccgacgg
    29941 ttgaatcaca aaaaatggaa cggtgcgctg ctcgggcgtg gtaccgccat gcccgccatc
    30001 ggcattgatg ccatgatctc ccgtaacaaa gacaatatac cctcgttcct tccattccat
    30061 gatgagtggc gcaagttttg aatcttgata aatggcatgg ttgcgatatt cttttgaatc
    30121 agcgccgaag gtctcgccat gatagtccat gcccattgga tgaagcagca aataatcggg
    30181 ggaaaattta cggatcaggt gtgcagcact ggcaaagagc tcaacatctg gatattcatc
    30241 ctccgtgtag aaacggccat gttgaatatt gagccgttca tcatcggtct ccttgtcatc
    30301 aactggatga tagggagcgc gattgtaaag ctctgaatac cagtaatatg ctgcagcagc
    30361 tgtggtcttt ccagcattct tcaacgtctg aaatacattc ggcttggcag agagtctgac
    30421 tatcgaattg gcaacaattc catgctcgct ggaaggcatg cctgtatgaa tggtttcata
    30481 catcgggcgg gacatgcttg gcaactcccc tacgatctta tggaggctgg cctgtttggt
    30541 ttcaacaagg tgccccaaaa agcccatgtt gttttttgca acgtcgtaac gtaaagcatc
    30601 tgaaagcaca agaatgactt tgttcatttt tagttcctca aacaaaaacg gttcgaagcg
    30661 cgccgcgcaa cgaaccgttt tgactttgat gggatcttct attcgtcgag caccaacacg
    30721 gcttcctttg aacaggtcac ctgttctttg gaaccaagtg caggcaattc gaggaatggg
    30781 ttgttgaagg tgtccatatt gaatttcgta ttcccgatct tcacctgaat acggacgaca
    30841 gaacccaaaa aggtgatgtt ttcgatggtc gcatcaacta cattggcctt cttttgatcg
    30901 gcggcaaaac tcaaacgctc aggtcggatg gcgatcttca ctttatcgcc tttcttgcga
    30961 ccttctgtgc cttcagcaga ctcaaactgg acgccatcca tgctgaccag cccattggca
    31021 gggtcaacca cttccgcatc cgcagtgttc aaagagccga cgaaattggc aacgaagcga
    31081 gtcttgggga agttgtagat ttcaaagggt gtacctacct gctccatcac gcccgtattc
    31141 ataaccacga tacggtcgga aatggacaag gcctcttcct gatcgtgcgt aacgaagatg
    31201 gcagtgatac ccaatttttt ctgaatggca cggatctcgg aacgaagaga gatgcggatc
    31261 ttcgcatcca acgcagaaag cggctcatcc agtaaaagca cctgcggatg gatggccagc
    31321 gcacgcgcca aagagacacg ctgctgctgc ccacccgaaa gctgatacgg gtatttattc
    31381 gcatgtttct caagattgat caggctgacc atttcttcca cgcgctgttt aatcgtcgct
    31441 tccggctctt tgcgaacccg aaggccaaag ccgatgtttt gggcaacggt catgttcggg
    31501 aagagtgcgt acgattggaa gatcataccg acattacgtt ggttcggggc tttatcggtg
    31561 atatctgctc cgtcaagcat gatcttgcca gaggtcggaa tttcaaaccc agccaccatg
    31621 cgcagggttg tggtcttgcc gcagcctgat ggcccaagaa aggaaacgaa ttcacccttt
    31681 tcaatatcga gattgaaatc atcaac
//
LOCUS       DAOQJC010000041        31223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_631_length_31223_cov_11.575013, whole genome shotgun sequence.
ACCESSION   DAOQJC010000041 DAOQJC010000000
VERSION     DAOQJC010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 31223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31223
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_631_length_31223_cov_11.575013"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1786
                     /locus_tag="PLE39_08180"
     CDS             95..1786
                     /locus_tag="PLE39_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HNE68343.1"
                     /translation="MTITLLDHLTFLYGENDSTRLLELTHSLLNNYRTRIEPRDGKST
                     ERDSILITYGDQIQTPTERPLQTLRKFCKTHLTDIVSGIHILPFYPWTSDDGFSVVDY
                     RQIDPALGNWDDVSSMQSFRLMFDGVINHISSQSAWFQGFLRDNPHYRDYFIMVEGDP
                     DLSKVVRPRALPLLTTFKTAAGEKKVWTTFSEDQIDLNFKNPEVLLEILDILLIYAER
                     GASFIRLDAIAYLWKEISTTCIHLPQTHHVIQFLRAALNEVAPHIQLITETNVPHADN
                     ISYFGDGTNEAQLVYNFALPPLTLHTFHTGNAGKLSNWARTLALPTDKTTFFNFLASH
                     DGIGINPVRSILSNEEINALVHKTLEHGGLISYKHNTDGTQSPYEMNINYFDALSDPN
                     AHEPIDLQVNRFIASQAIMLALIGIPGIYFHSLFGSRGWFEGVRQTGRNRTINREKLQ
                     LDELQNELTDEHSLRSKVFTKYSQLLKARSSSSAFHPHGAQTVLDSHPSIFAIERLSP
                     DGASRVLCLHNVCDQIISVSTGHKSAIDLFTGQAIQASDVRMLPYQVQWLKINQV"
     gene            1814..3157
                     /locus_tag="PLE39_08185"
     CDS             1814..3157
                     /locus_tag="PLE39_08185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE68344.1"
                     /translation="MTNPSHHIGFISTRFAGTDGVSLETQKWATVLERLGHRCFYFAG
                     ECDRSSDQSQVTPEAFYRHPEIEKINQQAYSGSWTVTQKARSEHPEIAALQKDFFSIY
                     VRPPQMTRRVHELKEHIKTELYKFARRFDLEILVVENAVTIPLNLPLGLAITEFIAET
                     GYPTIAHHHDFHWERQRFQVNCVGDYLAAAFPPTLPSIRHVVINSLQAQQIATRYGVT
                     SRVIPNVMDFDSPPPAPDAYTQNIRADFGIEQGEYFFLQPTRVIQRKGIEHAVELVRR
                     LGVPAKLVISHASGDEGSDYEHRVREYAHLLDVTVRFEADQVQDERGTTKDGRKIYTL
                     GDVYPHADLITYPSSIEGFGNAFLEAVYYRRPILVNNYSIYEVDIKPKGFRAVWFDGF
                     INVETLKKVREILLNPAEAQAWAEENYQLAKRYFSFTVLERRLQSILADCLGQQV"
     gene            3154..4368
                     /locus_tag="PLE39_08190"
     CDS             3154..4368
                     /locus_tag="PLE39_08190"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE68345.1"
                     /translation="MNIAILHYSVPPIVGGVESVIGHHARLMSADGHSVRLIAARGES
                     LSDQILLTEIPLADSRHERVTHMKNQLDHGEVTQDFESLRDELVFQLQTALADVDILI
                     AHNVCSLNKNLALTAALYQLHVSKKLPRLILWHHDLAWTTPRYQPELHDGYPWDLLRV
                     DWGGTTHVVVSHLRRRELSTLMKIPPDEIQIIPNGVDAARFYKLESQTQSLLEKTKLL
                     DAAPILLLPVRVTPRKNIELALHTLAELRKRFADAALVVTGPLGPHNANNQKYFESLS
                     SLRTQLGLQHSAHFLAELTDSFLPDEVIADFYRVADALLFPSREEGFGIPLIEAAFSH
                     LPAFCADIPPLRELGLNDAAFFSPDEDPVKISAMIADYFQSSPAARLSMRARAAFRWE
                     AIYRQHIAPLLK"
     gene            4384..6183
                     /locus_tag="PLE39_08195"
     CDS             4384..6183
                     /locus_tag="PLE39_08195"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HNE68346.1"
                     /translation="MFKHSEFTPIHNIIVPFIHEGPGLHALDAARQFDAEITLVGVVV
                     VPQDQSLSVGASAARALRRLLRVFGRDKRTTSKSQVIVSHEPWNELAKLLRDEKPDLL
                     CLEYESHFKAMCVTPSDALSRPPCDIALVRGKISSKPKQVLVPVRGGPHAELALRVGL
                     GLHVQGVTALHLRRTNDPEAGTDAPFKGLERVLKQMPEVQKQFEVTDDSAQFILDHAR
                     QKDVIILGTTIQPLTSSVSLGPVADRILREAPCAVIAVKSGRPIPHAVYDESAGVEAI
                     SILVDKWFAENTFHADEFNQLRELIELKKKQGLSISLALPALNEEETVGRVIKMMKKE
                     LLQRIPLLDEIVLIDSDSTDRTREIAKKEGVPVYIHQQLLGRLGARRGKGEALWKSLL
                     VTKGDIIVWIDTDIVNIHPRFVYGIIGPLLTNPQVQLVKGFYRRPLRVGQKMQAGGGG
                     RVTELTARPLLNLFYPELSGVVQPLSGEYAGRREALERAPFYSGYGVETGLLIDIYER
                     YGLNAIAQVDLLERIHHNQSLEALSKMSFAIIQTVMRKLENRVGRVMLEDVNRSMKLI
                     RYNNKGYSLDVEQIAELERPPMIEVQEYLERQK"
     gene            6315..7400
                     /locus_tag="PLE39_08200"
     CDS             6315..7400
                     /locus_tag="PLE39_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HNE68347.1"
                     /translation="MTNSFLLFLAGLILLILGADLLVRGASRLAAAFGISPLVIGLTI
                     VAIGTASPEIAVSLQAAANGQVDLTLGNVLGSNIFNILFILGVTSIVAPIAIAGQLIR
                     KDAPIMLVVSLFVLALSLDGNIGAIDGGLLLLLLAAYTAFALKQSRIESSEVQNEYNK
                     EFAKKEPRTAKGIVINFVSIAIGLGLLILGSNWLVDSAVAIAKSLGVSELVIGLTIVA
                     VGTSLPEVMTSVIAALKNESDIAVGNAVGSNIFNLLGVLGAGAFFSPNGITVAEQVLQ
                     FDFPVMVFAALVTLPIFYIDNRISQLEGALLLGYYSIYLGYIVLQATQSSFLPLFSLF
                     AAFFTPATFVGLVIIAIRTSGLMKKIS"
     gene            7471..7932
                     /locus_tag="PLE39_08205"
     CDS             7471..7932
                     /locus_tag="PLE39_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE68348.1"
                     /translation="MNVTLCMKRQVVSIPVTTTIREAAAIFTKKHIGLLPIVDKNNKP
                     VGVIGIRDLLKLEMPDFINFVVDVDFVHDFGAVEDTRPSAKTLNKSVKSLMRPVLTVE
                     EDCGLLRAYALMLQQNLHDIPVVSKDGQLIGIASRVDIGSTILSTWARVEK"
     gene            7932..9218
                     /locus_tag="PLE39_08210"
     CDS             7932..9218
                     /locus_tag="PLE39_08210"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HNE68349.1"
                     /translation="MVFPAILSTVIFLGSLILIFWEKINRSIIAIAGSVLMIGIGKVM
                     GFYSEEAAIEAIDFNTLGLLLGMMMLVAMLEPTGFFEYLAVLAARTSKGKPTQLFFLL
                     GSITTVLSMFLDNVTTVVLIAPVTILISEILGVSALPYLVTEALLSNTGGVATLVGDP
                     PNVLIGSAAGLSFNDFLVYSLPIVLVSWAGALFLLKHLFKKELSKRPRRSKAVMKLNP
                     AESFNQPETARKILIVLGGAILLFFVHHLLHIEPSFVALSAAAVALLWVQPDIHEILK
                     KVEWSVLIFFGALFVMVGGLEHAGTLEMAVGLFENASGWSPVLFGVLIIWIVAALSAV
                     VDNVPITIALIPVIKGLGETGIDITPLWWALAFGAGFGGSGTIIGSSANIIVATLSEK
                     TRTPITASLWIKRGLPVMLVTCAIASILYVLIFPWM"
     gene            9340..9675
                     /locus_tag="PLE39_08215"
     CDS             9340..9675
                     /locus_tag="PLE39_08215"
                     /inference="COORDINATES: protein motif:HMM:NF017920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HNE68350.1"
                     /translation="MENPIHLLLVAVVQDQDLDSATKAVQAIGAAVTYLSSSGGFLGR
                     RNATLLIGLPAEKEADALAALQEACRQRIEYMTLPLEGSPMPMPAPVPVTVGGATVFA
                     LPVERFEQI"
     gene            9728..9988
                     /locus_tag="PLE39_08220"
     CDS             9728..9988
                     /locus_tag="PLE39_08220"
                     /inference="COORDINATES: protein motif:HMM:NF017920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HNE68351.1"
                     /translation="MKLIIIIVKDNDADTLTQAFTAGNFRVTRVASTGGFMRSGVVTM
                     LLGVEDPQVDAAIQVVRDSLPSKGDQKRATLFVVPVQHFEQV"
     gene            10055..11284
                     /locus_tag="PLE39_08225"
     CDS             10055..11284
                     /locus_tag="PLE39_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68352.1"
                     /translation="MQKNIRFYSALLALFVVALACANPLGGTSPVEPAGVETAVAATF
                     QALTAPAAESGDAPPASTSDLLPHSIYFLNTDGAGLTQVHRLEKDGKTVTQITFEPAK
                     VEDYAVSPVDGSVVYTSNNQLLNVLADGSNRSLLVDGGKVDENNRYLTYISNPVFSPN
                     GQTIAFGYKGLSFYSIVSGQYNTVLANTFETLDGGYSFPLELFWPETYSADGSKLLIT
                     LGYMEGASAAVYYPNGGALVRLTGGEGAMICCGETEWTSDGSAFYAASPTFGMFSAGL
                     WRVDAATGAVTTLLNGSFDTNPLNLADEPFLAPDGQLYFFHTTMPGGTDMVNRAALQL
                     VRAGADGATNRTVLRPETFERMNEALWAPDASFVIVANADLPEIHQGGRLELYYTDGQ
                     KGMISLLPFGMDMKWGP"
     gene            complement(11350..12231)
                     /locus_tag="PLE39_08230"
     CDS             complement(11350..12231)
                     /locus_tag="PLE39_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE68353.1"
                     /translation="MKNRRYWRNLVLMLAFSLLLAFFIFLVFVSDQWAQARLHPARVI
                     PNDDLLVENGIPFQEIELNTKDGVTLSAWYTPPKNGAVILVAHGYNSSREAAWHLLFA
                     KHGYGVLSFDFRAHGLSGGEVSTLGYFEQADVEAALEYALSQPEVEHVGAWGGSMGAV
                     TVLLAAADRVEIEAVVSDSAFPTLEDVLAINVPFGLMKPFVMFFGEYRSGVDMDLVRP
                     VDDIARISPRPVFLIDGWEGGTIIMNSPYRLYDAAREPKQIWVEDGVPHLAMHAYFPE
                     DYENRVVGFFDEYLLAH"
     gene            complement(12228..13145)
                     /locus_tag="PLE39_08235"
     CDS             complement(12228..13145)
                     /locus_tag="PLE39_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE68354.1"
                     /translation="MDLRQQLGFVLKISGRVLLLAGISLLVLGGMGVYDVLHPPRIVP
                     PGYTLVKENIDFRSVDLLTEDGIRLSAWYTPPDDERKGVVILLAHGYGDNRPEWVYAM
                     IAKHGYGVLAWDARAHGESEGTISTIGYREVLDVKAALDFALAQPGVDHVGAWGGSMG
                     GATLIRATASLPQIEALVVDSSFSSLGEEVDFLAPYPFVNPLAKWMLTFVLDHDLYSA
                     SPLEKISKISPRPVYIIQGSADKVASPDSAFKLYNAAQDPRYLWVEEGARHLGTFQEN
                     PRRYERRVIRFFDEAFFGELARLFEQKQP"
     gene            complement(13148..13981)
                     /gene="panC"
                     /locus_tag="PLE39_08240"
     CDS             complement(13148..13981)
                     /gene="panC"
                     /locus_tag="PLE39_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNE68355.1"
                     /translation="MRTIISLDELKAARLSFNGTVGLVPTMGYLHEGHLSLIRRAKAE
                     CDHVVVSIFVNPTQFGANEDLAKYPRDLERDLSLIEPFGVDLVWTPTPEIMYPTGYQT
                     WVEVEAMTRPLEGAMRPTHFKGVTTVVAKLFNATQAHKAYFGQKDAQQVAVIRQMVWD
                     LNFPLEVVVCSTVREADGLAMSSRNKYLEGESRQAATILFRALTAAKEMYQTGERDAE
                     VLREKMKEVLGTEPLAQVQYVSCADYNTLEELDVVKGKTLLSMAVLIGKTRLIDNFVL
                     G"
     gene            14076..14291
                     /locus_tag="PLE39_08245"
     CDS             14076..14291
                     /locus_tag="PLE39_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68356.1"
                     /translation="MAYSHTNSKGTTYFLHQKGKMFFFSKEVKEGALDALPAGYTVSE
                     MKTGMLVLKRIVAEGEAKPAETDQPAS"
     gene            14397..14780
                     /gene="ssb"
                     /locus_tag="PLE39_08250"
     CDS             14397..14780
                     /gene="ssb"
                     /locus_tag="PLE39_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNE68357.1"
                     /translation="MPTLNRVQLIGRLGRDPESKFTPTGKKVTTFSVAISNRWKDRNG
                     EMKESTEWVNIEAWERLGEVCQEYLHKGSLVFLEGRLKTEKYEEKGETRYYTKVVLQS
                     LEFLDKRSAEEPMMAVEEDPGDYEA"
     gene            14864..15358
                     /locus_tag="PLE39_08255"
     CDS             14864..15358
                     /locus_tag="PLE39_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE68358.1"
                     /translation="MVVNFHELYKKYAQDVHRFAYWLCGNEQEAEDITSETFIRALTA
                     AENIRAETVKGYLLTIARNLALKKLKQAKRITDLTTNLPAAFEGLDRTVETRLSLQGV
                     MQFLQSLPEVDRAALLMHIQEDLPYEEIARALGISVASARVKVHRMRIKLNEYRMKTE
                     ESAP"
     gene            15355..15765
                     /locus_tag="PLE39_08260"
     CDS             15355..15765
                     /locus_tag="PLE39_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68359.1"
                     /translation="MKITRDVITDLLPVYLSGEASQDTKALVDAFLKDDPEFSKLVRE
                     NGAILPESQINLSKENEMDTLLKTQKLLRQRSTYMAFTILFTLLPLSFYIDAQGMHWM
                     WTDTPINAAIFAGFAIIFGFQYWRISQKLKGSDL"
     gene            complement(15892..18429)
                     /locus_tag="PLE39_08265"
     CDS             complement(15892..18429)
                     /locus_tag="PLE39_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE68360.1"
                     /translation="MNPLNTIQPLLDFWKRDEQTAPNFFAWQTTPPRPAQTHPIPTDV
                     PAPLREALSTRGIDQLYSHQLSAWTRSRAGKHIILSTGTASGKTLAYNLPVLAAMLQN
                     PEARALYLFPTKALAQDQLSTLANLKPETWNISPAIYDGDTPQANRPAIRKTARIVLS
                     NPDMLHTGILPHHANWSDFFSNLKFIVIDEGHTYRGVFGSHVANVLRRLKRVANFYGA
                     QPQFILASATIGNPKELAERLIEEEVELIDNDGSSRGERHFIIYNPPITDAALGLRKS
                     SLLESVRLATDLLNHNIQSVVFARTRRSVEIILTYLQSPIANLQSSTVNQVRGYRSGY
                     LPHQRREIEQGLRDGSVKTVVATNALELGIDIGGLGAAILVGYPGSIASAWQQSGRAG
                     RGDDPAVSVLVASPNPLDQFLAHHPEYFFGRSPERALVNPNHLLILLEHLRCALFELP
                     FQQGEGFGSISDELLDEYLRFLLANGEVHLSNEKYYWMKDQYPAANISLRSASPQGVV
                     LQSITEDGRPLTIGTVDGESAAWMVHPGAIYMHEAQQYFVQEYNIENRIAQLVPVGLD
                     YYTEAQKNSEIEVLGEAEQAIVPGGVKAFGEIQVTTQVVGFRKIRWFTYENLGQEPLD
                     MPPSELQTTGYWLSLSEEAVNSLRDAGVWTNDPNDYGPDWQKIRLAVRKRDQFKCQVC
                     GAVETVREHDVHHKIPFRSFIREGRVDREQANRLENLTTLCPSCHKKVEQNVRIRSGL
                     AGLGFVLGNLAPLFLMCDPRDLGTHTDPAWSAVNGQPSVVLYDLVPAGIGFSQKLFEI
                     HDELMQRACELVEQCACKDGCPSCVGPGGENGTGGKAETLAILKHLV"
     gene            complement(18430..19434)
                     /locus_tag="PLE39_08270"
     CDS             complement(18430..19434)
                     /locus_tag="PLE39_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNE68361.1"
                     /translation="MKKLFLVVVLFLGIAVVVLSFGELETILFTLRKAHLGYFLLAIL
                     IQMAWLLTTGRMYKSVYRLLGLDESILALSRIAAAANFINIVAPTAGMGGVALFAAEA
                     RRRGHPAGRVTVAAALFLSLDHAAFLCVLALGLLVLVRRNDLDASEISASLFMLGIAV
                     SYAFILYLGYRSEQKLGNLLAKMVRFINGIVRPFLKREYLSEIRAREFAHEVAEGFSG
                     LTGRPAVLVRPILWGLLDKILLMCILVCSFLSFEVPFSTGTIIAGFSIAYLFLIVSPT
                     PSGIGVVEGLMPVALSSLNVNWSQAVVITIAYRTVTFWVPFGLGAWAFRSLHSAGEEP
                     "
     gene            complement(19525..20217)
                     /locus_tag="PLE39_08275"
     CDS             complement(19525..20217)
                     /locus_tag="PLE39_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNE68362.1"
                     /translation="MFYFNPTYLIYMLPAFILMALTSWYVRSAYSKWSRVPVSSRLSG
                     AQAAQRLMSTGNLYGLQIRGVGGELTDHYDPRDKTLYLSQGVGNGNSVASVAIAAHEL
                     GHALQDAEEYFPLRFRSALVPLVNIGSNLGWILIMIGLFLNFTQLAWLGVIIFAGGAV
                     FALATLPVEFDASARAKRLLAETGIIQTEEEKRGVNAVLNAAALTYVAGLVTAILQLL
                     YYVSLVGGRRRS"
     gene            complement(20290..21621)
                     /locus_tag="PLE39_08280"
     CDS             complement(20290..21621)
                     /locus_tag="PLE39_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNE68363.1"
                     /translation="MNNDNTLKYVFAALAVIVLVLGAFAGGFVTGQIAQMAGLPSLPG
                     FEAPVVASAPTASPEQQNATPSEVQTLFAPFWEAWNIIHENYVDQPVDDLALMRGAIN
                     GMMQALGDKHSTYMDPSTYEQANSSLQGEYEGIGAYVDTTTKYLTITSPMPGSPAERA
                     GLEPGDQVVAIDGEDMTDTIAEVARNKILGPAGTTVHLTILREGAEGLLEFDVVREKI
                     TIKSATGKMLENDIAYVQITTFGSKTTSELLETLTDLMAQNPKGIILDLRNNGGGYLQ
                     TSVEVISQFVGDGVALYEQYGDGKRTTYDVLPGGLATDTNIPMVVLINEGSASASEIV
                     AGALQDYGRAKLVGVVSYGKGSVQNWIPLSNDNGAVRITIAKWLTPKEHTIHEIGLTP
                     DVFVEMTAEDYKAKLDPQLDAAIETLLAMINGTPIPTSMPTAVPSAVPTQP"
     gene            complement(21735..21959)
                     /locus_tag="PLE39_08285"
     CDS             complement(21735..21959)
                     /locus_tag="PLE39_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68364.1"
                     /translation="MESKTIAALLFIVVIVGINFVMYGIVRGFMTSGQKGRSMLETLS
                     KSLAPNVKNKDEEFQELRKKIEELEKGGQE"
     gene            complement(21959..22594)
                     /locus_tag="PLE39_08290"
     CDS             complement(21959..22594)
                     /locus_tag="PLE39_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HNE68365.1"
                     /translation="MKFTTIFFDLDDTLYPSTTNLWLAIKQRMNEFMRERMSIPEDEI
                     PALREKYYMQYGTTLKGLEKHHDINVADFLAYVHDLPLGNYLTPNPIQREVIASLPTR
                     NLIFTNADIPHAERVLTALNLRDLFSDIVDVTTVAPYCKPMPESFDIAMKIAGESDPS
                     RCVMIDDLRRTTRAARERGMFSILCDGTWSVDDADAYFTDWQDLKNILEAQ"
     gene            22651..24429
                     /gene="mutL"
                     /locus_tag="PLE39_08295"
     CDS             22651..24429
                     /gene="mutL"
                     /locus_tag="PLE39_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNE68366.1"
                     /translation="MPIRLLSSEVASQIAAGEVVERPASVVKELTENALDAGAKSISI
                     SIEDAGRTLVEIADDGHGIPANELELAAARHATSKLVQSEDLFHIQTLGFRGEALASI
                     GSVSHMTITSRVADAAEGARLKVDGGIAGKVEKVGAPVGTTLRVENLFYNVPARLKFL
                     KTDVTERRAIDALVTRYALAYPNVRFKVTDGKQVTLQTAGDGDRRSILAALYGVDVAK
                     QMLEVMAQDDGLSLTGFISPTSLTRSNRKEITFFVNGRWVQDYSLTTALLQAYHTLLM
                     VGRYPLTTLFLEVDPEDVDVNVHPTKAEVRFRSQDKVFSFIQRSARKALLAYTPVASV
                     SPQLWGGSKPASSEGREMGIDWSIGHDDELQVTGSKLQVENETSSSTQPANLPPSNVQ
                     PATFNTVPLLRLIGQIGSTYIVAEGPDGLYLIDQHAAHERVLFEKLMAQHDKKTIPSQ
                     ALLTPEIVTLPPQSAKALMPQLPFLNHFGFEVEEFGANTFQVRAMPALFAGGDPASAL
                     RALVEDFEEDEAPLQSEVEAKLAARVCKRLAVKGGQTLTTEEQRALLNDLEACASPRT
                     CPHGRPTMIHLSVDALERQFGRRGAR"
     gene            24496..25470
                     /locus_tag="PLE39_08300"
     CDS             24496..25470
                     /locus_tag="PLE39_08300"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE68367.1"
                     /translation="MKQLAETITATDAEIILENIHAMIALVEKEGLLISWNRAFEACK
                     KLFPQAKTLGDFFAPDDRDLIISKLNSRAREHWIIKLPLPAGRSEVVIQCDCHLVPLA
                     NGQMVFIAEEIHLDSAFQEVERLNRQVKLFKIESEQSKKLARRKQVEIEGVMAQAREV
                     AETDALTFLPNRRTIIRELQDEFIRAERYQNQFSISIVDVDHFKTINDTHGHMTGDEV
                     LRQIAIHLRDHVRHPDVVGRYGGEEFLILLPNSGQKAAEEQASRLCQLVREMKVVISN
                     GEVLNVTVSIGVAQLKHSEETWDSLLNRADNAMYEAKSKGRDGWAVAE"
     gene            25561..27546
                     /locus_tag="PLE39_08305"
     CDS             25561..27546
                     /locus_tag="PLE39_08305"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNE68368.1"
                     /translation="MLTLAEKILFVFATLVSLYFTYKGVMKIVGHISSGQGKADWSLL
                     WKRIGDLIVKVGLFQPVFRFRLGPSILHALIGWGFLSFLLINLADLIYAFTNYKLLEH
                     LGIFGDAYRLLADIANVAIIVGILAMAFRRFILRPATLSTRETTLLNPKARTGIMRDS
                     AIVATFIFVHNTMRFLGESFGVALAGHSDRWQPSISAAAGLWSGVDASVLVAGEHIAF
                     WLSIGAVVAFLPYFPYSKHIHLFFAPLNFALKPERKSIGELSYINLDDQSIEQFGAAK
                     MKDLGWEQIMDSYACIMCFRCQEVCPAYNTGKLLSPAALEINKRYHFNGGGTTDVAMT
                     ELISEEAVWACTSCGACVDICPVGNEPMRDILDIRRNLSMMESAFPKQLETAFKGMER
                     NQNPWNVSQGDRMKWAEGMNVPTIEQNAEPEILWWVGCAPATDSRAQKTAQAFAKILN
                     TAGVNYAVLGKNESCTGDSARRAGREDIFFGLASQNVEILNEVAPKRIVTTCPHCLHT
                     IKNEYPAFGGNYQVIHHTQLINELVGSGKISMNLEGDNMKITFHDPCYLGRHNKVTDA
                     PRDTLRSAGVETVEMPRNSAKSFCCGAGGAQMWKEEEQGSARVNITRFNEAKATGAST
                     VAVGCPFCLTMMTDAAKGDGGEIQVKDVAEIVAERLK"
     gene            27619..27969
                     /locus_tag="PLE39_08310"
     CDS             27619..27969
                     /locus_tag="PLE39_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68369.1"
                     /translation="MKSIERRNGYLYLEYAEPYTFENFITISKQVFETCKAEHYQKVL
                     VNVSEMTGKVKALERFEIGVQGALIFRNKVKIAVLYRAEEIDWFAETVSVNRGLNARI
                     FSEMEKAMEWLGVG"
     gene            28087..30504
                     /locus_tag="PLE39_08315"
     CDS             28087..30504
                     /locus_tag="PLE39_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNE68370.1"
                     /translation="MPNKKKGTIALPLEKDEIIKDYRLAYQSRQASIIGRREVLSGKA
                     KFGIFGDGKEIAQLAIARAFQKGDWRSGYYRDQTWMFMLGVMSIQEFFAQLYAHGDVT
                     YDPASAGRQMNAHFASRNLYPNGTWRPQTQMYNTASDISPTAAQMPRAVGFAYASVMY
                     RNIKELQDQKDFSINGNEIAFATIGNASTSEGLFWEAVNAIGVLKAPAIITVYDDGYG
                     ISVPNQFQMVKENIGALLKGFERDPFTPPDKVQNGYDHYTVRAWDYTALVETYLTAAE
                     IAREYHIPALVHVIDVTQPQGHSTSGSHERYKTPERLKWEEEFDGLKKMRAWMITNRV
                     ISAPELDSVEKEDYATVEGFRKAAWDAYLAPITEERNQVLDMLDEIAPTSNHVYELRL
                     IRDRLATLPSYSRRDIHSIVHEALRLLRDESHPSKQVLIQWKHENDSVNVERYGSHLY
                     SGTAMKVSEVKPEYSESSPTMFGFEIMNAAFDAALTRDPHIIAFGEDVGKLGDVNQGF
                     KGMQEKYGEFRVTDTGIREATIMGQAIGMAMRGLRPIAEIQYLDYVLYALQTMSDDLA
                     TLHWRTAGGQKAPVIVRTRGHRLEGIWHSGSPMAGILSLVRGMYVLVPRDMTHAAGFY
                     NTLLKSDEPAIVVEVLNGYRVKERLPKNIGEITLPLGVPEIIRDGKDVTIVTYGACCR
                     VVLDAADKLSKVGIDVEVIDVQSLMPFDIHGKIVESLKKTNRILFVDEDVPGGTTAYM
                     MQEVIEKQGGYFHLDSPARTLSAKAHRPAYGSDGDYWSKPNAETIFDAVYEMMNEVDP
                     GAYPLFS"
     gene            30553..30939
                     /locus_tag="PLE39_08320"
     CDS             30553..30939
                     /locus_tag="PLE39_08320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNE68371.1"
                     /translation="MEKEKISAKNYRELIVWQDAIKIAKAVYLLTGKFPKQETYALAD
                     QLRRAAVSVPSNIAEGQARKSPGDFRRFLHIALGSLAEVDTQLILAQEFGYLSKEDID
                     PMDMEIQNLRKKLYALINSLPSATNR"
     gene            30988..>31223
                     /locus_tag="PLE39_08325"
     CDS             30988..>31223
                     /locus_tag="PLE39_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNE68372.1"
                     /translation="MATKVLVPRLGEGVDEVTVTKWLKQVGDSINELEPLLEVNTDKV
                     DTEIPAPVTGTILQIIAQEGTPAKVGELLAIIGQP"
BASE COUNT         7869 a         8040 c         7887 g         7427 t
ORIGIN      
        1 tttcgtttta caggagattt aatcttaagc ctcaaaaatc atggtataaa aatttacgag
       61 gactcaaggt ttattcacca gccacaaagg actgatgacc ataacactgc ttgaccatct
      121 cacttttcta tacggggaaa atgactcaac gcgcctgctc gaactcacac atagtttgct
      181 caacaattat cgtacccgca tcgaaccccg cgacggtaaa tcaactgaac gcgattccat
      241 cctcatcact tatggcgacc agattcaaac accgaccgaa aggccgttgc aaacgctcag
      301 gaaattttgc aaaacccact taaccgatat tgtcagcgga atccacatcc tgcccttcta
      361 cccgtggaca tcagacgacg gcttttcagt ggtggattat cgccagatcg atcccgcgct
      421 tggcaattgg gacgatgtat cgtccatgca aagtttcagg ctgatgtttg atggggtgat
      481 caatcatatt tcatctcaga gtgcatggtt tcagggattc ctgcgtgaca acccacatta
      541 tcgtgattat ttcatcatgg tcgaaggtga ccctgatctt tcaaaggttg tccgtccgcg
      601 ggcattgcca ttgttgacga cgttcaaaac agcagcgggt gaaaaaaagg tttggacgac
      661 cttcagcgaa gatcagatcg atctcaattt caaaaacccc gaagtactgc tggaaatcct
      721 tgatattttg ctcatatacg ctgaacgcgg tgcaagtttt atccgccttg atgccatcgc
      781 atatttatgg aaggaaatca gcacaacctg tattcacctt ccgcaaaccc atcatgtgat
      841 ccaatttctg cgcgccgcat tgaacgaagt cgctccgcat attcaactca tcaccgaaac
      901 caacgttccg cacgcggata acatctccta ttttggggat gggacgaatg aagcgcaact
      961 cgtttacaac tttgccctgc cgccgctgac cttgcacacc tttcacacag gcaatgcagg
     1021 caaactgtca aactgggcaa ggactttggc cctgcctact gacaaaacga cctttttcaa
     1081 tttcctcgcc tcgcatgacg gtatcggaat caatcctgtg cgcagcatcc tctcgaacga
     1141 agagattaac gcattggttc acaagaccct tgaacatggc ggcttgatct cctacaaaca
     1201 caacacggac ggcacacaaa gcccgtatga gatgaatatc aattatttcg atgcactttc
     1261 cgacccaaat gcacatgagc cgatcgatct gcaggtcaac cgttttatcg cctcacaagc
     1321 cattatgctt gcgctgatcg gcatccccgg aatctatttt cacagcctgt tcgggtctcg
     1381 cggatggttt gaaggtgtaa gacaaacggg gcgcaatcgc acgatcaacc gcgagaaact
     1441 tcaactggat gaattacaaa acgaactcac agacgaacat tcattgcgct caaaggtttt
     1501 cacaaagtat tctcagctgt tgaaagccag aagcagttca tcggctttcc atccgcatgg
     1561 ggcacagacc gtcctggatt cgcacccgtc catttttgca attgaacgcc tttctcccga
     1621 tggtgcatca cgcgtgttgt gcctgcacaa cgtctgcgat caaatcattt ccgtctcaac
     1681 agggcataaa tcggcaattg acctttttac gggtcaggca attcaggctt cagatgtcag
     1741 gatgctgccc tatcaagtgc aatggctgaa aataaatcaa gtatgactgc actgaaaaaa
     1801 ttaaaggtgt tttatgacaa acccatctca tcacatcggt ttcatttcca cccgtttcgc
     1861 agggaccgac ggcgtttcgc ttgaaacgca aaagtgggca accgtgctcg aacggcttgg
     1921 gcaccgctgt ttctactttg ctggcgaatg cgatcggagc agcgaccagt ctcaggtcac
     1981 gccagaggcg ttttatcgtc atcctgaaat tgaaaaaata aatcagcagg cttattcggg
     2041 cagttggacg gtgacccaaa aagcgcgttc cgaacaccct gaaatcgcgg ctttgcaaaa
     2101 agattttttc tcgatctatg tgcgcccgcc gcagatgacg cgccgcgtcc acgagttgaa
     2161 ggaacacatc aaaacagagt tatataaatt tgcgcgccga tttgatctgg aaattttggt
     2221 ggtcgaaaat gccgtgacga tcccgcttaa tctgccgctt ggtcttgcca tcacagagtt
     2281 catcgccgag acgggatacc ccaccattgc acatcatcat gactttcatt gggagcgcca
     2341 gcggtttcaa gtcaactgcg tcggcgatta tcttgcagcg gccttcccgc ccaccctgcc
     2401 gtcgattcga catgtggtga tcaattcact gcaagcacaa cagatcgcca cgcgttatgg
     2461 cgtgacctca cgcgtgatcc ccaacgtaat ggatttcgac tctccgccgc ccgcgcccga
     2521 tgcctacacc caaaacatcc gtgcagattt tggcattgag cagggcgagt attttttcct
     2581 gcaacccaca cgcgtcattc aacgcaaggg cattgaacat gcggtcgaac tggtgcgccg
     2641 actcggtgtg cctgcaaagc tggtcatctc acacgcctcg ggtgatgaag gttcagatta
     2701 tgaacatcgt gtgcgcgaat atgcccattt acttgatgtg accgtccgtt ttgaagcgga
     2761 ccaggtgcag gatgaacgcg gcacgacgaa ggacggcaga aagatctaca cacttggcga
     2821 tgtgtacccg catgcagacc tgatcacgta tccatccagc attgaaggat ttggcaatgc
     2881 ttttttggaa gccgtatact atcgccgccc gattctggtc aacaattatt cgatttacga
     2941 agtggatatc aagccgaagg gatttcgcgc cgtgtggttc gacggattca tcaatgtcga
     3001 aaccttgaaa aaggtgagag aaattctgtt gaatcccgcc gaagcacagg catgggcgga
     3061 ggaaaattat cagcttgcaa agcggtactt ttcattcacc gtcttggagc ggcgattgca
     3121 atccatcctt gcagattgtt tggggcagca ggtatgaaca tcgccattct gcattattcc
     3181 gttccgccca ttgtcggcgg cgtggaaagc gtgatcggtc atcatgcgcg gctgatgtct
     3241 gccgatggac attctgtccg cttgatcgcg gcacgcggcg aatcgctgag cgaccagatt
     3301 cttctcaccg aaattccgct tgccgattcg cggcacgaac gtgtgactca catgaaaaat
     3361 caactcgatc atggcgaagt gacacaagac tttgaatctt tgcgcgacga actggtattt
     3421 caactgcaaa ccgctttggc cgatgtggat attttgatcg cacacaacgt atgttcgttg
     3481 aataaaaatc tggcgctcac cgccgcgctg taccaactgc atgtttccaa aaaactgccg
     3541 cgcttgatcc tctggcatca tgatctcgcg tggacaaccc cgcgctatca acccgaacta
     3601 catgatggct atccctggga tttgctgcgt gtcgattggg gtggaaccac gcatgtggtc
     3661 gtttcacact tgcgccgcag ggaattatca acgttgatga agatcccacc cgatgaaatt
     3721 cagatcatcc caaacggcgt agatgcggcg cgtttttaca aactcgaatc gcaaacacaa
     3781 agcctgcttg aaaaaacaaa attactggat gccgcgccga tccttttgct gcctgtccgc
     3841 gtgacgccgc gcaaaaatat tgaactggct ctgcacacgc tggcagaatt acgcaaacga
     3901 ttcgccgatg ctgcactcgt ggtcacgggg ccgctggggc cgcacaatgc caacaaccaa
     3961 aaatattttg agtcgctttc cagtttgcga acccagcttg gcttgcagca ttccgcacac
     4021 ttcctcgccg agttgaccga ctcttttttg cccgatgaag tcatcgcaga tttttatcgt
     4081 gttgcggatg cgcttttatt tccaagccgc gaggaaggat tcggcatccc gttgatcgaa
     4141 gccgcattca gccatttacc cgccttttgc gcggatattc ctccactccg cgagctggga
     4201 ttaaatgacg cagcattttt ctcccctgat gaggatcctg taaaaatctc agccatgatc
     4261 gcggattatt tccaatcctc ccctgctgcg cgtttgtcca tgcgcgcgcg ggcagccttt
     4321 cgctgggagg cgatctatcg tcagcacatt gcgccattat tgaaataaca aaaaggaaac
     4381 ctcatgttca agcactctga attcacgcct attcataaca ttattgttcc attcatccat
     4441 gaagggccag gccttcacgc tttggatgcc gcacgccaat ttgatgcaga gatcacgctg
     4501 gtgggtgtag tggttgttcc gcaggaccaa tcgttgagcg tgggcgcatc ggcggcgcgg
     4561 gccttgcgca ggctgctgcg cgtcttcggc agggataaac gcaccaccag caaatcgcag
     4621 gtgatcgtat cgcacgaacc gtggaatgaa cttgcaaaac tgctgcggga tgaaaaaccc
     4681 gatctgcttt gccttgagta tgagtctcat ttcaaggcca tgtgcgtcac ccctagcgat
     4741 gcactaagcc gcccgccatg tgacattgcc ctggtacgcg gaaaaatttc cagtaaaccg
     4801 aagcaggttc tcgttccagt aagaggcggg ccgcatgcag agttggcgct gcgtgtgggg
     4861 ctgggtctgc atgttcaagg cgtcacggca ttgcatctcc gccgcacaaa tgatcccgaa
     4921 gcagggacgg atgccccatt caaggggttg gaacgcgttc tgaaacaaat gcctgaggtg
     4981 cagaagcagt tcgaggttac ggatgattcg gcgcaattca tcctcgacca tgccaggcag
     5041 aaggatgtga ttattttagg aacgaccatc cagcctttaa cgagttccgt ttcactgggg
     5101 cctgtggcag atcgcatttt gcgtgaagcg ccctgtgccg tgatcgcggt caagagcgga
     5161 aggccgatac cacatgccgt gtacgatgaa agcgcaggcg tggaggcgat ctccattctg
     5221 gtggacaagt ggtttgcaga aaacacattt catgcagatg aattcaatca attgcgcgaa
     5281 ttgatcgaac taaagaagaa acaggggctg agcatcagcc tggccctgcc tgcacttaac
     5341 gaagaagaaa ccgtgggcag ggtgatcaaa atgatgaaga aggaattatt gcagcggatt
     5401 ccactgctgg atgagatcgt attgatcgat tcagattcca cagaccgcac ccgcgagatc
     5461 gccaaaaaag aaggtgtgcc tgtttacatc catcagcaat tgctgggacg gttgggggca
     5521 cgacgcggca aaggcgaagc cttgtggaaa agtctgttgg tgacaaaggg cgacatcatt
     5581 gtgtggatcg atactgacat cgtcaatatt catccgcgct ttgtgtatgg aatcatcggg
     5641 ccgcttttaa ccaacccaca ggtgcaattg gtgaaaggct tttaccgcag gccgctgcgc
     5701 gtgggacaaa aaatgcaggc aggcggcggt gggcgtgtga ccgaactgac ggcgcgcccg
     5761 ctgttgaatt tgttttaccc cgagctttcg ggtgtggtgc agccgctttc aggtgaatat
     5821 gcaggcaggc gcgaagcatt ggagcgcgca ccgttctact cagggtatgg tgttgaaaca
     5881 ggtttgctca ttgatattta cgaacgctac ggcctgaacg ccattgcaca ggtggatttg
     5941 ctcgaacgca tccaccataa tcaatcgctg gaagcgttga gcaaaatgtc cttcgcgatc
     6001 attcagaccg tgatgcgcaa attggaaaat cgggttggcc gtgtgatgct tgaagatgtg
     6061 aatcgatcca tgaaactgat ccgctacaac aacaaaggtt attctctcga cgtggagcag
     6121 atcgcagaac tggaacgccc gccaatgatc gaggtgcagg aatatttgga aaggcaaaaa
     6181 tagttttttt tctgcactca gaatcacaag attcaatttg tattgaccag ccctgtattt
     6241 aaagcggaat taatctcagg cggaaaaagt catggtataa aaatttcgga caattccatt
     6301 ggaagcacat tgacatgaca aactcttttc tccttttcct cgctgggctc attcttttga
     6361 ttcttggcgc agacctgctg gtgcgcggcg catcacggct ggctgcggcc tttggcattt
     6421 ctcccctggt tattggcctg accattgtgg caattggaac cgcatcacct gaaattgctg
     6481 tttcgctaca agccgcggcc aatggacagg ttgacttgac tctcgggaac gtacttggct
     6541 ccaacatatt caacattctc tttattcttg gggtcacatc cattgtagcg ccgatcgcaa
     6601 tcgcagggca attgatccgc aaggatgccc ccatcatgct ggtcgtctcc ttattcgtac
     6661 ttgcactttc attggatgga aatattggcg cgattgatgg tggtctgctt ttattactcc
     6721 tggccgcata cacagccttt gccttgaaac agagcaggat tgaaagcagt gaagttcaaa
     6781 atgagtacaa caaagagttc gcaaagaaag agcctcgcac cgccaaaggg attgttatta
     6841 actttgtttc tatcgccatt gggcttgggt tacttattct gggttcaaac tggctggtgg
     6901 attcagctgt tgcaatcgca aaatccctcg gtgtcagtga attggttatc ggattgacca
     6961 tcgttgcagt tggcacctct ctgcctgaag taatgacatc agtcattgct gcattaaaga
     7021 atgaaagcga tattgctgta ggaaatgcag tgggaagtaa tatctttaac ctgctcgggg
     7081 tgttgggtgc cggggcgttt ttctcaccca acggcattac tgtggctgaa caggtgctgc
     7141 aatttgattt tccagtgatg gtatttgcgg cacttgtcac tctgccgatt ttttacattg
     7201 ataatcgcat ttcgcaattg gaaggtgcct tgttattggg gtactacagc atctaccttg
     7261 gttatatcgt tttacaggcg acacaaagtt cattcttgcc tcttttcagc ctctttgcag
     7321 ctttttttac acctgccaca tttgtcgggc tggtcattat tgccattcgc acgtcaggtc
     7381 tgatgaaaaa gataagttaa aataaaaaag ttacccccaa atgacatcat acaaaatatc
     7441 acaggtcaac catccttttc tggagattcc atgaatgtaa ctttatgcat gaagcgtcag
     7501 gttgtttcaa tccccgtcac gacaaccatc cgtgaagccg cagctatttt tacaaaaaag
     7561 catattggct tgctgccaat tgtagataaa aacaataagc ctgtgggggt gattggtata
     7621 cgtgatctgc tcaaactcga aatgccagac ttcatcaatt tcgttgtaga cgttgatttt
     7681 gtccatgatt ttggtgcagt ggaagatacc cgcccatctg caaaaacgct gaacaaatct
     7741 gtcaagtcgt taatgagacc agtacttaca gttgaagaag attgtggtct tttacgagcc
     7801 tatgcactga tgttgcaaca aaacctgcat gatattcccg ttgtttccaa agatggtcaa
     7861 ttgattggca ttgcatcacg ggtggatatt ggatcgacca ttctttccac atgggccagg
     7921 gtggaaaaat gatggtattc cctgccatcc tctctacagt tattttttta ggcagcttga
     7981 ttcttatttt ctgggaaaaa atcaaccgtt ccataattgc gattgcaggt tctgttctga
     8041 tgattgggat cggaaaggtc atggggtttt attcagaaga agctgccatt gaagccattg
     8101 acttcaatac actcggtctc ctgttgggca tgatgatgct tgttgccatg cttgagccaa
     8161 cgggcttctt cgaatatctt gcggttctgg cggcacgtac ttcaaaagga aaaccaaccc
     8221 agttattttt tctgttggga agtatcacta cagttctttc aatgtttttg gataacgtca
     8281 ccacggttgt cttgattgcc cccgtcacca ttcttatcag cgaaattctg ggggtcagcg
     8341 cactgcccta tcttgtcact gaggcattat tatcgaacac aggcggtgtt gcaacgctgg
     8401 tgggagatcc gcccaatgta ttaattgggt ctgcagcagg attatctttc aatgactttc
     8461 tggtgtattc acttcccatt gtgcttgtat cctgggctgg ggcgctcttt ctcctgaaac
     8521 acctattcaa aaaggaatta tccaaacgtc cgcgcaggag taaggctgtt atgaagctaa
     8581 accctgccga gtcatttaac cagcctgaaa cagcacgtaa aattttaatt gtacttggcg
     8641 gagccatttt gctttttttt gtgcatcatt tgctccacat tgaaccatca tttgtagcac
     8701 tcagtgcggc ggctgttgcc ctgctctggg tgcagcctga tattcatgaa attttgaaaa
     8761 aagtggaatg gagtgtattg atctttttcg gggctctatt tgttatggta ggcggtttgg
     8821 aacatgctgg aacactggaa atggccgttg gtttatttga gaatgcctcg ggttggtcgc
     8881 ctgttttatt cggtgtcctt atcatttgga ttgttgcggc tctttcggca gtggtggata
     8941 atgtgccaat taccattgcc ctaattcccg tcatcaaagg gttgggagaa acagggatcg
     9001 acatcacccc tctttggtgg gcgcttgctt ttggcgcggg cttcggcgga agtggaacta
     9061 ttattggctc aagcgcaaat atcattgttg caactctctc agaaaaaaca cgcaccccca
     9121 tcactgcttc cctgtggata aaacgcggcc tgccagtgat gctggtcact tgcgctattg
     9181 caagcatttt gtatgtcctc attttccctt ggatgtgatt ctgatttcaa ggttgaagca
     9241 tctccataca tctgataata tcgaagtgag gaaatgccgc tcgtaccaga atccattttc
     9301 ggttaagggc gaagcgtcta ttgtcaaagg agaacgaaga tggaaaatcc aatccacctc
     9361 ttattggttg cggtcgtgca ggatcaggac cttgattctg cgaccaaagc tgtgcaggca
     9421 atcggtgcgg ccgtcaccta cctttcgagt tcggggggct ttttagggcg gcgcaatgcc
     9481 accttgttga tcggcctgcc cgcggaaaaa gaagccgatg cgcttgccgc actgcaagag
     9541 gcctgccgtc aacgcattga atacatgacc ctgccgctcg aaggctcgcc catgcccatg
     9601 cctgcgcctg tgccggtcac cgtcggcggc gcaactgtgt ttgcattgcc tgttgaaaga
     9661 ttcgaacaaa tataaaatgc aaagtcctga aggtttataa aacctctcgg cttaaggaga
     9721 agtcatcatg aaattgatca tcatcattgt aaaagacaac gacgccgaca cgctcacaca
     9781 ggcattcacg gcagggaatt ttcgcgtgac gcgtgttgcc tccacaggtg gttttatgcg
     9841 cagcggtgtg gtcaccatgt tgttgggcgt cgaagacccg caggtggatg cagcgataca
     9901 ggtcgtgcgc gattcgctgc catccaaagg tgaccagaaa cgtgccaccc tgttcgtggt
     9961 tccagttcaa cacttcgagc aagtgtaacg ctgacttcaa caacaaagac gttactggtt
    10021 taacgtcttt gttttgttta acgataaggt caaaatgcaa aaaaacatcc gtttctattc
    10081 cgcgcttctt gctttgttcg ttgtcgctct tgcctgtgcc aatccattgg gtggaacatc
    10141 gcccgttgaa cctgccggtg tggaaaccgc cgtcgctgcg acatttcaag cgctgacagc
    10201 tcccgccgcc gaatcgggcg atgcgcctcc tgcttcgacc tcggacctgc ttccgcattc
    10261 gatctatttt ctcaacaccg acggtgccgg gctgacacaa gtccatcgcc ttgaaaagga
    10321 cggcaaaacc gtcacccaga tcacttttga acctgcaaag gtggaggatt atgccgtttc
    10381 acctgtggat ggcagtgtgg tgtacacctc caacaatcaa ttactcaatg tccttgccga
    10441 tggaagcaat cgcagcctgc tcgtggatgg cggcaaggtg gatgaaaaca accgctacct
    10501 cacttatatt tccaatcccg ttttttcgcc gaacggccag acgatcgcct ttggatacaa
    10561 aggattaagt ttttattcca ttgtgtcggg tcaatacaac actgtgctgg caaatacatt
    10621 cgaaaccctg gatggcggct attcatttcc ccttgaatta ttctggcctg aaacctactc
    10681 tgccgatgga agtaaattgc tcatcactct cggctacatg gaaggtgcat ctgccgcggt
    10741 ctattacccc aacggcggcg cactcgtccg cttgactggc ggtgagggag ccatgatctg
    10801 ttgcggcgaa accgagtgga cttcagatgg ttctgctttt tatgcggcca gccccacctt
    10861 tggcatgttc agcgcgggcc tctggcgcgt ggatgcagcc acaggtgcgg tcacaactct
    10921 gttaaatggc agtttcgata ccaacccgct caatctggcc gatgaaccct tcctcgcacc
    10981 ggatggacag ttgtatttct tccacaccac catgcccggc ggcacagaca tggtcaaccg
    11041 cgcagcgttg caacttgtcc gcgctggggc cgatggagcc acgaatcgaa ccgttctacg
    11101 ccctgaaacc tttgagcgca tgaacgaagc cttgtgggcg ccagatgcaa gttttgtcat
    11161 cgtagccaat gccgacctgc ctgaaatcca tcaaggcggc aggcttgagc tgtattacac
    11221 cgacggccaa aaaggcatga tctccctcct cccctttggc atggatatga agtggggacc
    11281 gtaatttttt tgaactactg aggcacagag ttgggaatgc aggttggatt gacaatccga
    11341 cctgcattcc taatgagcca aaagatattc atcgaagaaa cccaccaccc tgttttcata
    11401 atcttctgga aagtaagcat gcatcgccag gtgcggaacg ccatcttcca cccaaatttg
    11461 ttttggctct ctggcggcat catacaaccg atacggggag ttcatgatga tcgtgccgcc
    11521 ctcccaaccg tcgatgagaa agaccgggcg cgggctgata cgtgcaatat catccacagg
    11581 acgcacgagg tccatatcca cgccgctgcg atattcgccg aagaacatta caaatggctt
    11641 catcaaacca aacgggacat tgatcgccag cacatcttcc aaagtgggaa aggcgctgtc
    11701 gctgaccact gcttcaattt ccactcgatc ggccgcagcg agcaacacag tcacagcacc
    11761 catggatcca ccccatgcgc caacatgctc cacctcgggc tgactcaagg catattccaa
    11821 cgcggcttcc acatcggcct gttcaaagta ccccaatgtt gaaacctctc caccgctcag
    11881 cccgtgtgcg cgaaaatcga aagacaaaac gccataccca tgtttagcaa acaaaagatg
    11941 ccaggccgcc tcacggctgg agttatatcc atgtgcgacc agtatcacag ctccattttt
    12001 gggcggtgtg taccatgctg aaagtgtcac accatctttg gtgttcaatt cgatctcttg
    12061 aaacgggatt ccgttttcta caagcagatc atcgtttgga atcacacgcg caggatggag
    12121 ccgcgcttgt gcccactggt cactgacaaa aacgagaaaa ataaagaaag ccagcaaaag
    12181 cgaaaacgca agcattagaa ccaggtttcg ccagtagcgc ctgtttttca tggctgtttt
    12241 tgctcgaaca aacgcgccaa ttctccaaag aaggcttcat cgaaaaagcg gatcaccctt
    12301 ctttcgtacc tgcgcgggtt ttcctgaaat gtccccaaat ggcgggcacc ctcctccacc
    12361 cataaataac gcggatcttg cgctgcgtta tacaacttga acgctgaatc aggcgaagcc
    12421 accttatctg cactgccttg aatgatgtac acggggcgcg gactgatctt gctgatcttc
    12481 tccaacgggc tggcgctgta taaatcgtga tcgagaacaa aagtcagcat ccattttgcc
    12541 agcggattca cgaacggata cggcgccaaa aaatcaacct cttcccccag cgaagaaaag
    12601 gaactatcca ccaccaaggc ttcgatctgg ggcaggctgg ccgttgcacg gatcaacgtt
    12661 gcaccaccca tcgaaccacc ccatgccccc acatgatcca caccgggctg cgccaaggca
    12721 aaatcgagcg cggctttcac atccaacacc tcgcgatatc caatggtgct gatcgtacct
    12781 tcgctttccc cgtgggcgcg ggcatcccag gcaaggactc catatccatg tttggcgatc
    12841 atggcataca cccattcagg tcggttgtcg ccgtaaccat gtgccagcag gatcacaacc
    12901 cctttgcgct catcatctgg cggggtatac cacgctgaaa gccgaattcc atcctcggtt
    12961 agcaggtcca cgcttctaaa atcgatattc tcttttacga gtgtgtatcc cggaggcacg
    13021 atccgcggcg gatgcaggac atcatacaca cccatgccgc ctaaaaccaa aagactgata
    13081 ccagccagca gcagcactct gcctgaaatt ttcaaaacaa aacccaattg ctgacggagg
    13141 tccattttta ccccagcaca aaattatcga tcaacctcgt tttgccgatc agcaccgcca
    13201 ttgaaagcag ggttttcccc ttcaccacat ccaactcttc gagggtgttg taatcggcac
    13261 aggagacgta ctgcacctgt gcaagcggct cggtaccgag cacttccttc attttctcgc
    13321 gcaagacctc agcatcccgc tcacccgttt gatacatttc cttggcagcg gtcagggcgc
    13381 ggaataagat cgtggccgcc tgacggcttt ccccttcaag atatttgttt cggctcgaca
    13441 tcgccaaccc atctgcctcg cgcacggtgg agcaaaccac cacttcgagg ggaaaattca
    13501 aatcccacac catctgacgg atgaccgcca cctgttgtgc atccttttga ccaaaataag
    13561 ccttgtgcgc ctgagttgca ttgaacaact tcgccacgac cgtggtcacc cctttgaaat
    13621 gggttggcct catggcccct tccagcggac gggtcatcgc ttccacctca acccaggtct
    13681 ggtagcccgt cgggtacatg atctcagggg tgggagtcca caccaaatcc acgccgaaag
    13741 gctcgatcaa gctcaaatcg cgttcaaggt cacgcggata cttggcgagg tcttcgttcg
    13801 ccccaaactg ggttgggtta acgaagatgc tgaccaccac atggtcacac tccgcttttg
    13861 cacggcggat aagggaaaga tgcccctcgt gcagatatcc catcgttgga accagaccca
    13921 ccgtcccgtt gaaagagagg cgagcggctt tcaattcgtc tagactaatt attgtcctca
    13981 tactattatc ctactctccc ctgtgctttg ggggcttgac aggctagaaa ctttgttcta
    14041 cactctactt accaaattag atataaggag aaaaaatggc atactcacac accaactcaa
    14101 aaggtaccac ctacttcctg caccagaaag gcaagatgtt cttcttctcc aaagaggtca
    14161 aggaaggcgc gctggatgct cttcccgctg gatacaccgt ttctgaaatg aagacaggca
    14221 tgctcgtcct caagagaatc gttgctgaag gcgaagcaaa gcccgcggag acagatcaac
    14281 ctgcctcata aagtataact ctgttcgaca caaccgattg ttcttttaga taaaaaggag
    14341 cctattatga cacgtaaaca agcatctaaa agccttgaag cgaaagcggg gatgacatgc
    14401 caacactaaa ccgcgtccaa ttgattggcc gcctgggcag agacccggaa agcaagttca
    14461 cccctacagg caaaaaagtg accaccttca gcgttgcgat cagcaatcgt tggaaagaca
    14521 gaaacggtga aatgaaagaa tccaccgaat gggtcaacat agaagcctgg gagcgtcttg
    14581 gagaggtttg ccaggaatac ctgcacaaag gaagcctggt ttttctcgaa ggacgcctga
    14641 aaaccgaaaa atacgaagaa aagggcgaaa caaggtatta caccaaggtt gtacttcaaa
    14701 gccttgaatt ccttgataaa cgctcagccg aggaacccat gatggccgtg gaagaagacc
    14761 ccggcgatta cgaggcctag tcaataaaaa ggacagctgt caaaagacgg ctgtccttct
    14821 ttttacctgt aacattttcg atctctgggg ataatatatg taaatggtcg ttaactttca
    14881 cgaactgtac aaaaaatatg cccaggatgt gcatcgcttt gcctactggc tttgtggaaa
    14941 cgaacaggaa gcagaggaca tcacctctga aaccttcatt cgtgccctca ccgccgccga
    15001 aaatatccga gcggaaaccg taaaaggtta cttgttgacc attgcccgca accttgccct
    15061 caaaaagctc aaacaagcca aacggatcac cgacctgacc acaaacctgc ccgccgcctt
    15121 tgaaggtctt gatcgaacgg ttgagacaag gctcagcctg caaggcgtca tgcaattcct
    15181 gcaatccctg ccagaagtgg accgtgccgc actgctcatg cacattcaag aggacctccc
    15241 ttatgaggaa attgcccggg cactgggaat ttcggtggct tctgccaggg tcaaggtcca
    15301 caggatgcga atcaaattga acgaatatcg aatgaaaaca gaggaatccg ccccatgaaa
    15361 attacacgcg atgtcatcac cgatctcctg cctgtatatc tttcaggcga agccagccaa
    15421 gataccaaag cgcttgtgga tgcatttctg aaagacgacc ctgaattctc aaaactcgtc
    15481 agagagaacg gcgccattct ccctgaaagt caaatcaacc tatcaaagga aaatgaaatg
    15541 gatacacttc tcaaaaccca aaagctgtta cgtcagcgtt cgacctatat ggcatttacc
    15601 atcctcttca cacttctgcc cctctcattc tacattgatg cacaaggcat gcattggatg
    15661 tggacagaca cgccgatcaa cgctgcgatc tttgcaggct tcgcgatcat ctttggattt
    15721 caatattgga gaatctcaca aaaattaaaa ggctccgacc tgtaaactaa gtaagtactg
    15781 aattcaattt cagtagaaat tcgcccttgc attttgcaag atttgtggga aaatatcgtt
    15841 gctccagtca gggtgccccc ctcaccctga ctggagcatt tttatttcca tttacacaag
    15901 atgtttcaaa atcgccagtg tttcagcttt tcccccggtg ccattctctc caccggggcc
    15961 cacacaagag ggacaaccat ctttgcaggc acactgctcc accaactcac aggcacgctg
    16021 cattaattca tcatgtattt caaataattt ctggctgaag ccaatgcccg caggaacaag
    16081 gtcgtagagg acaacagagg gctgtccgtt caccgcagac catgcggggt cggtgtgagt
    16141 acccagatcg cgcggatcgc acataaggaa gagcggtgca aggttgccca atacaaaccc
    16201 gagcccggcc aacccgctgc ggatgcggac attttgctcc acttttttat ggcagcttgg
    16261 gcaaagtgtg gtgaggtttt ccaaacggtt ggcctgctca cgatcaacac ggccctcgcg
    16321 aataaacgag cgaaatggga ttttatgatg gacatcatgc tcgcgaacag tttccaccgc
    16381 gccgcatacc tgacatttga actggtcccg ttttctgacg gcgagtcgta tcttttgcca
    16441 atcagggccg taatcgttgg ggtcattggt ccacacaccg gcatcgcgca ggctgttcac
    16501 ggcttcttca gaaagcgaaa gccaatagcc tgtggtttgc aactcagagg gcggcatatc
    16561 aagcggttcc tgtcccaaat tttcatacgt aaaccagcgg atcttgcgaa acccaaccac
    16621 ctgagtagtg acttggatct cgccaaatgc tttcacgccg ccaggcacaa ttgcctgttc
    16681 tgcttcgccc aacacttcga tctctgaatt cttttgtgcc tcggtgtaat aatccaaacc
    16741 cacaggtaca agttgggcga tacggttttc aatgttgtat tcctgcacga aatattgctg
    16801 ggcttcatgc atgtagatcg cccccgggtg aaccatccag gcggctgatt cgccatccac
    16861 ggttccgatg gtcaagggcc ttccatcctc cgttatgctt tgcaatacca ctccctgcgg
    16921 cgaagccgaa cgcaatgaaa tattggcagc agggtattgg tctttcatcc aataatattt
    16981 ttcgttggaa aggtgaacct caccgttcgc aagcaggaag cgaagatatt catccagcag
    17041 ttcatcagag atgctgccaa acccctcacc ttgttgaaat ggcagttcaa aaagggcgca
    17101 gcgcaaatgc tccaacaaaa tcaacagatg attcggattc accaacgccc gttccggtga
    17161 cctgccgaaa aaatattcgg gatggtgtgc aaggaattga tcaagcggat tgggggatgc
    17221 gaccaacacc gataccgcag gatcatcccc ccgccccgcc cgacccgatt gctgccatgc
    17281 gctggcgatg gaacccggat acccgacaag gattgccgcg ccaagtccgc caatgtcaat
    17341 tcccagctca agcgcattgg ttgccacgac ggtttttacg gagccatccc gcagtccctg
    17401 ctcgatctcg cggcgttggt gcggaagata ccctgaacga tatccccgaa cttgatttac
    17461 cgtggacgat tgaaggttgg caatcggcga ttgaagatag gtgaggatga tctctacact
    17521 gcggcgcgtt cttgcaaaca caacagactg gatgttgtga ttaagtaaat ctgtcgccag
    17581 gcgtacactt tcaagcaggg agctttttct caaccccagg gcggcatcgg tgatgggcgg
    17641 attgtagatg ataaagtgac gctcaccgcg ggaagagccg tcattgtcta tcagttcaac
    17701 ttcctcttcg atcaaacgct ctgccagctc tttcgggtta ccgatggtgg ccgaagccag
    17761 aatgaattgc ggttgtgcgc cgtagaaatt cgccacccgc ttcaacctgc ggagcacatt
    17821 ggcgacatgc gagccaaaga caccacgata ggtgtgtccc tcatcaatga cgatgaattt
    17881 caagttggag aaaaagtcgc tccaatttgc gtggtgcgga agaatccccg tatggagcat
    17941 atctgggttc gaaagaacaa tgcgtgccgt tttgcggatg gcaggacggt tggcttgcgg
    18001 ggtatcaccg tcataaattg caggtgatat gttccaggtc tcaggtttca ggtttgcaag
    18061 cgtggatagt tgatcctgcg cgagggcttt ggtggggaag agatacagcg cgcgcgcttc
    18121 agggttttga agcatggccg caaggacagg caggttgtag gcaagggtct ttccgctggc
    18181 cgtgcctgtg gaaaggatga tgtgtttccc agcccgtgac cttgtccatg cggagagttg
    18241 gtgggagtaa agttggtcaa taccacgggt ggataatgcc tctcgaagcg gagcaggcac
    18301 atcggtcggg atgggatgcg tttgcgcggg gcgcggaggg gtggtttgcc atgcaaaaaa
    18361 attcggggcg gtttgctcat cccgtttcca gaaatcgagt agaggttgaa tggtattcaa
    18421 aggattcaat tatggttctt cacctgcgct gtgcagggag cggaatgccc atgcgcccaa
    18481 cccgaacgga acccaaaatg taactgtgcg ataggcaatg gtgatgacga ccgcctgact
    18541 ccagttgaca ttgagcgaag acaatgccac cggcatgagt ccttccacaa cgccaatacc
    18601 ggaaggggtg ggcgaaacaa tgagaaaaag atatgcgatg gaaaacccgg caataatcgt
    18661 acctgttgaa aatggaactt caaaggaaag aaatgaacag accagaatgc acatcagcaa
    18721 gattttatcc aggagccccc acagaatggg tctgaccaga acggctggcc tgcctgtcaa
    18781 ccctgagaaa ccttctgcaa cttcatgggc aaattcacgt gcgcgaattt cactgaggta
    18841 ttcgcgtttg aggaacggcc tgacaatgcc attgataaaa cgcaccattt tggcaagcag
    18901 gtttccaagc ttttgttccg aacgatatcc aaggtacaaa atgaaagcat aagacacggc
    18961 aattcccagc atgaaaagtg atgcggaaat ttcgctggca tccagatcgt tacggcggac
    19021 aagcacaagc aaccccaatg ccagcacaca aagaaaagca gcatggtcaa gcgagaggaa
    19081 gagcgcagcc gccacggtaa cacggcctgc aggatgtccg cgtctgcggg cttcggcggc
    19141 aaaaagggcc actccgccca ttcctgcggt aggtgcaacg atgttgatga aattggcggc
    19201 agcggcaatg cggctcagtg caaggatgga ttcatcaagc ccaagcaggc ggtataccga
    19261 tttatacata cgccccgtgg taagcagcca ggccatttga atgagaattg ccagaagaaa
    19321 atatcccagg tgggcctttc gcaaagtaaa caggatggtt tcaagttcgc caaagctcag
    19381 cacgaccacg gcaatgccga ggaagagcac aacaacaagg aataattttt tcatcgcaaa
    19441 tgattataca cttcggatgg aacaaaaaac gaacccaccc aatcacactt aaggggggtt
    19501 cgtcacttta tttacttaag atgatcagct tcggcgtctg ccgcccacca acgatacata
    19561 gtacagtaat tggaggatgg cggttacaag gcctgcaaca taggtcaagg cagccgcgtt
    19621 cagaacggcg ttgactccgc gcttctcttc ttcggtctgg atgatgccag tttcagcgag
    19681 caatctcttt gcccgtgcag aggcgtcaaa ttccacgggc agggtggcca gagcaaagac
    19741 ggcaccaccg gcaaatatga tcacacccag ccaggccaac tgtgtgaagt tgaggaacaa
    19801 accgatcatg atcaggatcc agccgaggtt ggagccgatg ttgacgagcg gtaccagtgc
    19861 cgagcggaag cgaagcggaa aatactcctc ggcatcctgc aaggcatggc caagttcatg
    19921 tgcggcaatt gccactgagg ccacggaatt tccattgcca acgccttgcg aaaggtaaag
    19981 ggttttatcg cgtgggtcgt aatgatcggt aagttctccg cccacaccac gaatctgcaa
    20041 gccatacaaa ttaccagtgg acattaaccg ctgtgcggct tgcgcgcccg aaagcctgct
    20101 gctgacgggg acacggctcc attttgaata ggcagaccgg acataccatg aagtcagcgc
    20161 catcaagatg aaagctggaa gcatatatat caagtaggtt ggattaaagt aaaacatttt
    20221 ttctcctgct attattacgt aaaacagttg taccagttgt tcaaagcagt acgaaaccac
    20281 tttgccaaat tatggctgtg tgggaacggc actgggtact gcggtgggca ttgaagtggg
    20341 gatcggtgtg ccgttgatca ttgcaagcaa ggtttcgatg gcggcatcca attgcgggtc
    20401 gagtttggct ttgtaatctt ctgcggtcat ttcaacaaag acatccgggg tcaggccgat
    20461 ttcgtgaatg gtgtgttctt ttggtgtcaa ccactttgca atggttatgc gcacagcgcc
    20521 gttgtcattg ctaagcggaa tccagttttg cacagaaccc tttccataag acaccacccc
    20581 caccagtttc gcacggccat aatcctgcaa ggcgcctgcc acaatttcag atgcagaggc
    20641 ggagccttca ttgatcaata caaccatggg aatgttcgta tcggtggcaa gtccgccggg
    20701 gagtacatcg taggtcgtgc gtttgccgtc accatattgt tcataaagag ccacgccatc
    20761 cccaacgaat tgggagatca cttccacaga agtttgcagg tatccgccgc cgttattgcg
    20821 caaatcaaga atgattccct tggggttttg cgccatcaag tcggtgagcg tttccagcaa
    20881 ttctgaagtg gtttttgagc caaaggtggt gatctgaaca taggcaatat cgttttcaag
    20941 catcttgcct gtggccgatt tgatggtgat cttttcacgt accacatcaa attcgagcaa
    21001 gccttccgca ccctcgcgca aaatggtcaa gtggaccgtt gtgcctgccg ggccgagaat
    21061 tttattgcgt gccacctcgg caatggtgtc ggtcatgtct tcgccatcaa tggcaaccac
    21121 ctggtcaccg ggttcaagcc cggcgcgttc ggcgggagag cctggcatgg ggctggtaat
    21181 ggtgaggtat ttagtggtgg tatccacata ggcgccaatg ccttcatatt caccctgcag
    21241 gctggaattg gcctgttcat aggtggatgg gtccatataa gtggagtgtt tgtcacccaa
    21301 agcttgcatc atgccgttga tggcaccacg catcaatgca aggtcatcca caggttgatc
    21361 gacataattt tcatggatga tgttccaggc ttcccagaac ggagcaaaaa gggtttgaac
    21421 ctctgacggg gtggcgtttt gctgttcggg ggaagcggtc ggcgcactgg caacaaccgg
    21481 cgcctcaaac cccggcaggc tgggcagccc cgccatttgt gcgatctgcc cggttacgaa
    21541 tccacccgca aacgcaccca atacgagcac gatcacggcc aatgcagcga aaacatactt
    21601 taaagtgttg tcattattca cagatgcctc caaatttggc taactaagtg gaaagatacc
    21661 actcggggat taatgtttgc tgaaatcttt gtacgatttg tgtaagaggg cttttccccc
    21721 atcggtaacg gaagttattc ctgcccgcct ttttccagtt cttcgatctt tttgcgcagt
    21781 tcctgaaatt cctcatcctt gtttttcacg ttcggggcaa gcgatttact cagggtttcc
    21841 aacatgctgc ggcctttctg cccggaggtc atgaatccgc gcacgatgcc gtacatgacg
    21901 aaattgattc caacgatgac tacaatgaac aaaagtgcgg cgatggtctt tgattccatt
    21961 attgtgcctc caaaatattt ttcaggtcct gccagtcggt gaaatacgcg tcggcgtcat
    22021 ccacgctcca ggtgccgtcg caaaggatgc tgaacatgcc gcgttcgcgg gcggcgcggg
    22081 tggtgcggcg caggtcgtct atcatcacac agcgcgaggg gtcactttca cctgcgatct
    22141 tcatggcgat gtcgaacgac tcgggcatgg gtttgcaata cggcgcgacc gtggttacat
    22201 cgacaatatc ggagaagagg tcgcgcaggt tgagggcggt cagcactctt tcggcgtggg
    22261 gaatgtctgc gttggtgaag atcaggttgc gggtcgggag cgatgcgatg acttcgcgtt
    22321 gaatcgggtt tggggtgaga taattgccga ggggcaggtc atgcacgtaa gccagaaaat
    22381 ccgcaacatt gatatcgtga tgtttttcca accctttcaa cgtggttcca tattgcatgt
    22441 aatatttttc gcgcaaagcg gggatctcat cctcagggat gctcatgcgt tcgcgcataa
    22501 attcgttcat gcgttgtttg atggcgagcc acagatttgt agtggacggg tagagggtat
    22561 cgtcgaggtc gaagaagatg gtggtaaatt tcattcggcg attatacccg cctccaattt
    22621 gcgcaaactg caagcggtta taatcgcgtc atgcctattc gtcttttatc ctctgaagtc
    22681 gcttcgcaaa ttgcggcggg tgaagtggtg gaacgccccg cttcggtcgt taaagaatta
    22741 actgaaaacg cgctggatgc gggtgcgaaa agcatttcca tttcgatcga agatgcaggg
    22801 cggactctgg ttgaaattgc agatgacgga cacggaattc ctgcgaacga gttggaactc
    22861 gctgcggcac gtcatgcgac ctcgaaactg gttcagtccg aggacctgtt ccacatccag
    22921 acattgggct tccgaggcga ggcgctggct tcgatcggtt ctgtctcgca catgaccatc
    22981 acttcgcggg tggcagatgc tgcagaaggt gcgcgcctga aagtggacgg cggcattgca
    23041 ggcaaggttg aaaaagttgg cgcgcccgtg ggcacgaccc tgcgcgtgga aaatcttttt
    23101 tacaacgtgc ctgcacgttt gaagtttttg aaaaccgatg tgaccgagcg ccgcgccatt
    23161 gatgcgctgg tcacgcgcta tgcgctggca tacccgaatg tgcgtttcaa agtgaccgac
    23221 ggaaaacaag tgacattgca aaccgctggt gatggagaca ggcgttcgat ccttgcggcg
    23281 ttatacggtg tggacgtggc aaagcagatg cttgaagtga tggcacagga tgatgggctg
    23341 tctctcacgg gttttatcag ccccacgtcg ctgacccgct cgaaccgcaa agagatcacc
    23401 ttcttcgtca atgggcgctg ggtgcaggat tattcattga ccaccgcctt actgcaggct
    23461 tatcacaccc tgctgatggt gggacgctac ccactgacaa cattgtttct ggaggttgat
    23521 ccagaagatg tggatgtgaa cgtccacccg acaaaggcgg aagtacgttt tcgaagccag
    23581 gacaaggtct tcagttttat tcaacgctcg gcgcgtaaag ccctgctggc ctacacgcct
    23641 gtggcttcgg tctccccgca attatggggc ggatcaaaac ccgcgtcctc tgaaggccga
    23701 gagatgggga tcgattggtc gattgggcat gatgatgagt tacaggttac aggttccaag
    23761 ttgcaggttg aaaatgaaac ttcgtccagc acgcaacctg ccaacctgcc cccttcaaac
    23821 gttcaacctg caaccttcaa taccgttccc ctgctgcgtc tcatcggtca gatcggctcg
    23881 acctacattg tggcggaagg gccagacgga ttgtatttga tcgaccaaca tgcggcgcat
    23941 gagcgggttt tgttcgaaaa attgatggca caacacgata aaaaaacgat tccatcacag
    24001 gccttgctca cgcccgaaat cgtgacacta cctccgcaat cggcaaaggc gctgatgccg
    24061 caactgccgt ttttgaatca cttcggcttt gaggtggagg aatttggcgc gaataccttt
    24121 caagtccgcg ccatgcctgc cttgtttgcg ggcggtgacc ctgcatctgc gctgcgggca
    24181 ttggtggaag atttcgaaga agatgaagcg cccctgcaat ctgaggtgga agcaaaactg
    24241 gcggcgcggg tttgcaaacg gctggcggtc aaaggcgggc agactctcac caccgaagaa
    24301 cagcgcgcgc tgttgaatga ccttgaagcc tgcgcttccc cgcgcacctg cccgcatggc
    24361 aggccaacga tgatccactt gtcggtggat gcgctggaac gtcaatttgg gagaagaggg
    24421 gcgaggtaat catgggttgc ttgctaaatt tgtaaggtgg acataaatat ttattgaatg
    24481 ggctacaatg gagatatgaa acagctggcg gaaaccatca cagccaccga tgcagagatc
    24541 atccttgaaa acatccatgc catgatcgca ctggttgaaa aagaaggatt gttgatttca
    24601 tggaaccgcg catttgaagc atgcaaaaag ttatttccgc aggcaaaaac attgggggat
    24661 tttttcgcac cagacgacag agatctgatc atttcgaaat taaattcacg agccagagaa
    24721 cattggatca tcaaactccc cctgcctgca ggcagaagtg aagttgtcat tcaatgcgat
    24781 tgtcatctgg taccgctggc aaacggacaa atggttttta ttgccgagga aatccacctt
    24841 gattccgcat ttcaggaagt ggaacggctg aacagacagg tcaaattatt taaaatcgag
    24901 agtgagcaaa gcaaaaagct cgcacgccga aagcaggtgg agatcgaagg agtgatggct
    24961 caggcgcgtg aagtggccga aaccgatgca ctgacttttc tgccaaaccg ccgcaccatc
    25021 attcgtgaat tacaggatga attcatccgc gcagaaagat atcagaatca attttcgatc
    25081 tcgatcgtgg atgtggatca tttcaaaacc atcaatgata cccacggaca catgacaggt
    25141 gatgaagtgc tccgtcaaat cgcaatccat ttacgtgacc atgtacgcca ccctgatgta
    25201 gtcggtcgtt acggcggcga agaattcctc atcctgctgc cgaattcagg tcaaaaagct
    25261 gctgaagaac aggcatcacg gttgtgtcag ttggtccgag agatgaaggt cgtgatcagt
    25321 aatggggagg tattgaatgt gacggtcagt atcggtgtgg cacagctaaa acacagtgaa
    25381 gaaacctggg attccctgct caaccgcgcc gataacgcca tgtacgaagc caagagcaag
    25441 ggacgagacg gctgggccgt ggcagaataa caaacgaaac caagccgcct cgcgcggcta
    25501 tttttttatc tccccatttg tgctaaactt aacgaacatt tcttatattt tggagtttcc
    25561 atgctgaccc ttgcagaaaa aatccttttt gtctttgcaa cactcgtttc gttgtacttc
    25621 acctacaaag gtgtgatgaa aattgtcggg catatctcaa gcggacaggg caaggcagat
    25681 tggtcactct tatggaaacg gattggtgac ctcatcgtta aggtggggct gttccaaccc
    25741 gtttttcgtt tcaggctcgg acccagcatt ctgcatgcgc ttatcggctg gggttttctt
    25801 tccttcctcc tcatcaacct tgcggatctg atctacgcat ttaccaatta caaattactg
    25861 gaacatctgg gaatttttgg cgatgcctac cgcctgctcg cggacattgc aaacgtcgcc
    25921 atcatcgtcg gcattctggc gatggcgttc cgccgcttca tccttcgacc tgcgactctg
    25981 tccacacgtg agacgacatt gctcaacccg aaagcgcgga ctggcatcat gcgtgattct
    26041 gcgatcgttg ccacgttcat cttcgtccac aacaccatgc gctttttggg agaatcgttt
    26101 ggggtggcgt tggcaggtca ctctgacaga tggcaaccaa gcatctcagc agcggcaggt
    26161 ttatggtcag gggtggatgc gagtgttttg gtggcaggcg agcatatcgc attctggctg
    26221 tccattggtg cggtcgtcgc attcctgcct tatttcccgt attcaaaaca cattcacctg
    26281 ttcttcgcgc ctttgaactt tgctttgaag cctgaaagaa aatcgatcgg tgaattaagt
    26341 tacatcaacc tcgacgatca atccattgag caattcggcg cggcaaagat gaaagacctc
    26401 ggctgggagc agatcatgga cagctacgcc tgtatcatgt gcttccgctg tcaggaagtt
    26461 tgccccgcat acaatacggg caaattgctc tcccctgccg cgttggagat caacaaacgc
    26521 tatcatttca acggcggcgg cacgaccgat gtcgccatga ccgaacttat cagcgaagaa
    26581 gcggtctggg catgcacctc atgcggcgcc tgcgtggaca tttgccctgt gggcaatgaa
    26641 ccgatgcgcg acattctcga tatccgccgc aacctatcca tgatggaaag cgccttcccc
    26701 aaacaattgg agaccgcatt caaaggcatg gagcgcaacc agaatccgtg gaatgtttcg
    26761 caaggcgacc gcatgaaatg ggccgagggc atgaatgtgc ccaccattga gcaaaatgcc
    26821 gagcccgaaa ttttatggtg ggtgggctgc gcccctgcaa cagacagccg cgcacaaaaa
    26881 actgcacaag cctttgcaaa aatccttaat acagcgggtg tcaactatgc tgtacttgga
    26941 aagaatgaat cctgcacagg tgattctgcc aggcgtgcag gccgtgaaga tattttcttc
    27001 gggctggcct ctcaaaacgt tgagatattg aatgaggttg cgccgaaacg catcgtgaca
    27061 acctgtccgc attgtttgca caccattaag aacgagtacc ctgcctttgg cggaaattac
    27121 caagtcatcc atcacacaca attgatcaat gaattggtcg gttcagggaa gatctcgatg
    27181 aaccttgaag gcgacaacat gaagatcacc ttccatgacc cgtgctattt ggggcggcac
    27241 aacaaggtca ctgatgcgcc gcgcgacaca ttgagatcag caggcgttga aacagtggaa
    27301 atgccgcgca attcagccaa atcgttctgt tgcggtgcag gcggtgcgca gatgtggaaa
    27361 gaggaggaac aaggctcagc gcgtgtgaat ataacgcgct tcaacgaagc aaaagcgaca
    27421 ggcgcaagca ctgtggcagt aggatgccca ttctgcctca ccatgatgac cgacgcagca
    27481 aaaggcgatg gtggagaaat acaggtcaag gatgtggcgg agatcgtcgc agaaagatta
    27541 aagtaaaaag taaccgcaaa cggagatgac ctgaaaaatc atctccgttt atatttaccc
    27601 caaaaccgag ggtcgagcat gaaaagcatt gaacgcagga atggatatct ctatcttgaa
    27661 tatgccgaac catatacatt tgagaacttc attaccatct cgaaacaagt attcgaaacc
    27721 tgcaaggccg agcattatca aaaagtactg gttaatgtat ccgaaatgac ggggaaggta
    27781 aaagcgttgg agcgtttcga gatcggggtg cagggcgctt taatcttccg caacaaggtg
    27841 aagatcgccg ttctataccg cgcggaggaa atcgactggt tcgctgaaac tgtcagcgtc
    27901 aatcgcggtt tgaatgcccg catattcagc gagatggaaa aagcaatgga gtggctgggg
    27961 gtaggttgaa aagtaaccag agacagcggg cgggcagaaa tgtccgccct tttctttctt
    28021 ccttcttctt tcttcatccg ttcccgttta taatccgccc aacaaacttt acaggagaga
    28081 gagttcatgc ccaacaaaaa gaagggtacg attgcgcttc ccctagaaaa agatgaaatc
    28141 atcaaagatt accgccttgc ctatcaaagc aggcaggctt caattatcgg caggcgtgaa
    28201 gttttgagcg gcaaggcaaa gttcggcatt ttcggcgatg gaaaagagat cgcccagctt
    28261 gccattgccc gcgccttcca aaagggcgac tggcgttctg gatattatcg cgatcaaacc
    28321 tggatgttca tgcttggcgt gatgagcatc caggaatttt ttgcgcagct gtacgcgcac
    28381 ggcgatgtca cctacgaccc cgccagcgcg gggcggcaaa tgaacgccca cttcgcatcg
    28441 cgcaatcttt atccaaacgg cacatggcgc ccgcaaacac agatgtacaa caccgcgtca
    28501 gatatttccc ctaccgccgc gcaaatgccg cgagccgtag ggtttgcgta tgcctcggtc
    28561 atgtatcgca acatcaaaga actgcaagat caaaaagatt tttcgatcaa cggcaacgag
    28621 atcgcctttg ccaccatcgg caatgcatcc acatctgaag gcttgttctg ggaagcagtc
    28681 aatgccattg gcgtattgaa agcgcccgcc atcatcaccg tgtacgatga tggctacgga
    28741 atctccgtcc ccaatcaatt tcaaatggtc aaggaaaata tcggtgcgct gctcaagggc
    28801 tttgagcgcg acccattcac gccacccgac aaagtgcaaa acggatacga ccattacacc
    28861 gtccgcgcgt gggattacac cgccctcgtc gaaacctacc tcaccgccgc tgagatcgcc
    28921 cgcgaatatc acatccctgc gctcgtgcat gtcattgatg tgacccagcc acagggacat
    28981 tccacatcgg gcagccatga gcgctacaaa acaccagagc gactcaaatg ggaggaagaa
    29041 tttgacggac tcaaaaagat gcgcgcttgg atgatcacaa accgagtcat ctctgcgccc
    29101 gagcttgatt ctgttgaaaa ggaagattac gccaccgtgg aaggattccg caaagccgca
    29161 tgggatgcct acctcgcccc catcaccgaa gagcgcaacc aagtgttgga catgctcgac
    29221 gagatcgcgc ccacctcgaa ccatgtctac gagttgagac tgattcggga tcggctggca
    29281 actctcccgt cgtattcgag gcgtgacatc cattcgatcg tgcatgaagc gctgagattg
    29341 ctgcgggacg aatcgcatcc atcgaagcag gtcctcatcc aatggaagca tgagaacgac
    29401 agcgtgaacg tggagcgtta cggttcgcat ttgtattctg gcacagcgat gaaagtgagc
    29461 gaggtcaagc ctgagtattc tgaatcatcg ccgaccatgt tcggcttcga gatcatgaat
    29521 gccgccttcg atgccgcgct gacaagagac ccgcacatca ttgcctttgg tgaagatgtg
    29581 ggcaaactcg gcgatgtgaa tcagggcttc aaagggatgc aggaaaaata cggcgagttc
    29641 cgtgtgaccg atacaggcat ccgcgaagcg acgatcatgg ggcaggcaat cggcatggca
    29701 atgcgtggac tgcgaccgat tgccgaaatt caataccttg attatgtgct gtacgccctg
    29761 caaaccatga gcgatgacct tgccaccctg cactggcgca ccgcaggcgg acaaaaagct
    29821 cccgtcatcg tgcgcacacg cgggcaccgc ctcgaaggaa tctggcattc ggggtcaccg
    29881 atggctggca ttttgagttt ggtgcgcggc atgtatgtgt tggttccgcg tgacatgacc
    29941 catgccgctg gcttttacaa caccctcttg aaatcggacg aacctgccat tgtggtggaa
    30001 gtgctgaatg gctatcgcgt caaagaacgc ctgcccaaaa atatcggcga gatcaccctg
    30061 ccgctcggtg tgcctgaaat tattcgtgac gggaaagatg tcaccatcgt cacctacggc
    30121 gcttgctgta gagtcgtgct cgacgccgca gacaaactca gcaaggtcgg cattgatgtg
    30181 gaagtgatcg acgtgcaatc gctgatgccc tttgacatcc acggcaagat cgttgaatcg
    30241 ctgaaaaaga ccaaccgtat tttgtttgtc gatgaagatg tccctggcgg cacaaccgcc
    30301 tacatgatgc aggaagtcat cgaaaaacaa ggcggctact tccatttaga ctcccccgcc
    30361 cgcaccttat ctgccaaagc ccatcgcccc gcgtatggaa gcgacggcga ttactggtcc
    30421 aagccgaatg ccgagacgat cttcgatgcg gtatacgaga tgatgaatga ggttgatcca
    30481 ggggcgtacc cgctgtttag ttgatgtgaa catggttggt ggttgggagt tggtggttga
    30541 tagtgaggct ttatggaaaa agaaaaaatt tctgcaaaga attatcgtga gttgattgtc
    30601 tggcaggatg caatcaagat tgccaaggca gtttacttac taactggaaa attccccaag
    30661 caggaaacct atgctctcgc agatcaactt cgccgcgctg cagtttctgt tccgtcaaat
    30721 attgcagaag gtcaggcaag gaaatcacct ggagactttc gaagattctt acacattgcg
    30781 cttggctccc tggcggaagt ggacacacaa ttgatactgg cacaagaatt tggatatttg
    30841 agcaaagaag atattgaccc catggatatg gaaattcaaa acctgcgtaa aaaactttac
    30901 gcgcttatca atagcctacc atcagccaca aaccgttaac catcaactac caactactaa
    30961 ccgtaaacta ccaactggag aaacccaatg gcaaccaaag tactcgtacc cagactcggc
    31021 gaaggcgtgg acgaagtgac cgtgacaaaa tggctcaagc aggtcggcga ctcgatcaat
    31081 gagttggagc cgctactcga agtcaacact gataaggtgg ataccgaaat ccccgcgccc
    31141 gtgacgggaa caattctgca gatcatcgca caggaaggca cccccgccaa agtgggagaa
    31201 ttgctcgcga tcattggtca gcc
//
LOCUS       DAOQJC010000042        30030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_656_length_30030_cov_10.693845, whole genome shotgun sequence.
ACCESSION   DAOQJC010000042 DAOQJC010000000
VERSION     DAOQJC010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 30030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30030
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_656_length_30030_cov_10.693845"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..341)
                     /locus_tag="PLE39_08330"
     CDS             complement(90..341)
                     /locus_tag="PLE39_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68373.1"
                     /translation="MEFSMNTGLGGILAFIAGIWVWIKLFQKEGVMKGILGFLCMLYT
                     FIWGLQNMKNEELKLKTPMYVWIAGIVLGIIINAVGSGG"
     gene            complement(372..791)
                     /locus_tag="PLE39_08335"
     CDS             complement(372..791)
                     /locus_tag="PLE39_08335"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNE68374.1"
                     /translation="MEHSIKTLWHQPVFSNLLTNRTETTAIIGFGVVHLGLSLAGISF
                     WTCPILAATGVPCPGCGLTRATMQLFHGEVAASLQTHAFAPIFLAALLLMISVLILPE
                     PARKKIISFVNRLETRNGVTAWVFFSLVLYWAFRLIA"
     gene            complement(846..1892)
                     /locus_tag="PLE39_08340"
     CDS             complement(846..1892)
                     /locus_tag="PLE39_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE68375.1"
                     /translation="MAESPKPAHLLIVDDNKVNRMVLSRSLEQQGHIVETAENGREGL
                     DKFRTGTFDLMLLDIEMPEMNGFEVLEACLNDVDLRQIPIIMTSAMEELDAVVKCVEL
                     GAEDYLTKPVNPILLRARVNASLEKKRLRDEQRRLFRTFATKEVAEQLLRSGFSLGGK
                     QVKASIMFADIRSFTSIAENQEPSESIDLLNHYFSLMFDAITNNGGTVNQVVGDGLMA
                     VFGAPIFFEDHSERAVRAALQMLEQLKVFNHNQAAHGKTQIRIGIGIATGQMVAGYTG
                     TQTRAIYTCIGDTVNVASRIEEYTKEALHPILIDNYTRQNLPADILVEDLGKIVFKGK
                     NQAINVFAVMGSSA"
     gene            complement(1898..2230)
                     /locus_tag="PLE39_08345"
     CDS             complement(1898..2230)
                     /locus_tag="PLE39_08345"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNE68376.1"
                     /translation="MSVIDLNTFTNLKETVGADFIGEMIDTFLEDALTQIGQMKSGLA
                     AQDVDLFRRAAHSLKSNAATFGATELAGLARELEMLGREKNLEIGNRLEVLQDAFEQV
                     RGQLLGMR"
     gene            complement(2281..7086)
                     /locus_tag="PLE39_08350"
     CDS             complement(2281..7086)
                     /locus_tag="PLE39_08350"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE68377.1"
                     /translation="MPKTEKAKKTTPKTAKKPTSAASRSGSSERIRKELKQREAELAI
                     IKSVQDGLASKLELQAVYELVGDKIHNIFGAQTTLIASFDLKTETQYFNYYIDRFGRE
                     NLEPTAMSGFTKTIARLKKTFLFNDHVKEQMEAYGAKLVLGPLIPKSALYVPLISGGE
                     VCGVISLQNMDRENSFSPSDVSLLETLASSMSAAMENARLLSETQRLLKETEQHNAEL
                     EAIRKASIDLSSNLEYSTVLDSILKSTSELMPSIENINLFLYENDELVFGTAIANGEI
                     KSTPVSAPRPGGMTDTVAKTGEIMLVEDMRKHPLYKDSPARWTGALIGLPLKFRHRVV
                     GVMNIHFTEARAFANTELRLLQLFADQASIAIENSRLFNESQRLLEESTQRGAELEIV
                     NSVQAKLASQLDGDTLYSLVGEKIAGIFDVQVVMIVSYDAEKNICSFPYLMEMGKRLN
                     QEPLPPSGISGYVLRTHQPVIINANMSNEEERLLGTRSSILGGGEDIKSRMDVPMLVG
                     NQVKGVISLQNVDRENVFKESDLRLLQTLANSMAIALENARLFDETQRRARESSVLNE
                     VGRDISSTLDLKAVMDKIAHHARDLLSASTSAIYLPQGDGSYFKAIAAEGEIAEEIKV
                     DTIYFGQGIIGGLAKTGQGEFINDTAHDPRTIQIPGTPVETEERLMAAPLLTGGKVGG
                     MMAVWRNGGHPFIEADLDFLKRLSLQAAIAIQNASMFDFINKRANELSIINALISGLD
                     PKLDIQTIYDDVGDQIQKIFDAQTVVLTMYDKETNITQFPYIFENGERFYQEPLVLKD
                     GMGGFSGHVIRTGEPIVANRNFDELSQKYNSVLLGANPGDVVVRSGVWVPMSVAGEVR
                     GVISLQNLEHEEAFSEADVRLITTIANSLSVTIENARLFDETQRLLKETKRRASQLEI
                     LNNIAQVLTQQLDVQTIVERVGDKIRELVMEDNVGIGLYDPEARSITAHYVTRKNERI
                     QTPSFPINDSTISASMLGKSLVINRQSPSLWKRLGSNMTALNEVPKSVIMVPMVVGKD
                     LIGGITIQNFERENAYDESIITLMESIASSMATAIQNARLFEDARLARAAAEQANEAK
                     SSFLATMSHEIRTPMNAVIGMSGLLLDTKLDDEQRDYAETIRNSGDALLAIINDILDF
                     SKIEAGRMDIEHHPFDLRDCVESSLDLVSARAVEKGIDLAYIFEGDIPPAIVGDVTRL
                     RQIITNLLSNAVKFTEKGEVVLTVSSQRYRNGGAKEKALLTFSVRDTGIGLSKDGMSR
                     LFQSFSQADSSTTRKYGGTGLGLAISKHLAEMMGGTMWATSDGLGRGSTFIFTVKAEL
                     AEILPAQKRGHSGIQPELQNKHVLIVDDNSTNRYILNAQTSKWGMIPHETASPLTALK
                     WIEQGEAFDVAILDMHMPEMDGLELATKIRAKAKFPLVLFSSLGRREAGDETNVFAAY
                     LTKPIKQSQLFDTLVGLFFRPSAYEEPPTTGRFKPDPQMAVRHPLRILLAEDNAVNQK
                     LALRLLEQMGYRADVASNGIEAIQSIERQQYDVVLMDVQMPEMDGLEATRQIVARWVN
                     KRPFIIGLTANAMQGDREMCLGAGMNHYIPKPIRVVELVDALLKARRVS"
     gene            complement(7258..8451)
                     /locus_tag="PLE39_08355"
     CDS             complement(7258..8451)
                     /locus_tag="PLE39_08355"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNE68378.1"
                     /translation="MEDYKQLIRERILARETFVRATFSGGQKGFSIQWEKVIVRPVEI
                     QGEVQLQFSYFDEKKDTTQNYTFKQAKAKVEDLLALPFRNMFVESNAGNVQVNVSKKG
                     KPIVNEFKPAAVPLTVNLAHNREKHRILSDENARPFLEAVGIMTRDGRIKADMQRKHK
                     QINEFLRLLEETASFSAWSGAPINVVDFGCGSAHLTFAVYYYLRNILNLNARVTGVDV
                     KAELIERHNQTAAAMGWDGLSFQVGYIAEYEPEVKPNVILALHACDTATDDALAKGIQ
                     WESKVIVCAPCCQHELQEQLSHAPTPSQMQPVFHHGILFERMGDILTDTFRATMLRVL
                     GYRTDVTQFVPIEHTGKNLMIRSLKTTQPGDPRWVEDYRNMKSFWQVRPYLEKLLGEA
                     YAQYF"
     assembly_gap    8487..8586
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8587..8804)
                     /locus_tag="PLE39_08360"
     CDS             complement(<8587..8804)
                     /locus_tag="PLE39_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68379.1"
                     /translation="MQDFPQPTDLGNGLSWTAKFIDWEWARYGRFYFLVDLYQGGIAI
                     KQLKIYTDDYGYGDPNRKQTNAELMERVR"
     gene            complement(8810..>10471)
                     /locus_tag="PLE39_08365"
     CDS             complement(8810..>10471)
                     /locus_tag="PLE39_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE68380.1"
                     /translation="SGGNMSTYDVIVIGSGAGGLTAAVALAQAGKKVIVLEQHDRPGG
                     WTHSFTLEGYRFSPGVHYIGSLGEGGGLRRIYDGLGVSQDLTFVELNPDGYDHIFIGE
                     KRIDFPKGKENLIERLKSHFPHEAKGIDSYFTDLTNMMEGLRRIGNLSNPLETAKSAG
                     NILKWIRSTGADLINAHISDPVLRGVLAAQSGDHGMPPSQVSAFIHAGITHHYLDGGF
                     YPLGGAFTIPRAFVRALKKSGGEIRLQCRVKRILLEGKKVLGVELESGEEIRADVVIS
                     NADPEVTFGKLIGKENLSARLKRKIDSVQYSTSCLSLFFATDMDLRGAGLDSGNMWYY
                     DHADVDKIYTQGLTDAVLNDETPPGMFLTTTTLKDPSKMHHGQHTCESFAFVGYDAFE
                     KWANSKYGARPADYEAMKEDLAWRMVRGLEKRIPGLSKHITFYSLGTPLTNEHYLNAT
                     RGNLYGIDKRPSQVGPLGFTPRTEFEGLYLCGQSTISHGVAGVTQSGIDAAKAVLNCR
                     ARDILTHTGTSPVFLQSENVSAWPEHLKKKMERGEAAKEEEEREV"
     assembly_gap    10472..10571
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10573..11448)
                     /locus_tag="PLE39_08370"
     CDS             complement(10573..11448)
                     /locus_tag="PLE39_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68381.1"
                     /translation="MITIENVTKRYDNTTVVHELNLQIHPGEIVGIIGHNGAGKSTTM
                     KMIAGLIEPTSGVVRVMGEDIQKGNARIKQKIGYLPEESPLYEAMTAQQYLLFFSELY
                     QMPRQKALKRIDDLLNSLGLHETKKLTGEFSKGMKRKTAIARALLHDPDLLILDEPNS
                     GLDPLTSFFIINYLKTLRREGKTILLSAHNLFHVETICDRVGIIKNGKLLIFDTMEAI
                     RARLGKREYQVIFHADQKLDYEEVNGNYIFRTAEVDGIAHMLETVSANKWTLMDLSMR
                     ESALEEIYVKLMTNE"
     gene            11577..13202
                     /locus_tag="PLE39_08375"
     CDS             11577..13202
                     /locus_tag="PLE39_08375"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNE68382.1"
                     /translation="MNQILVIAWREITRLRKRFGGGASPLAVLALLAVLGGSSYFLRD
                     AVSLGSGLYRVGVMGNVPTINDSRFAVMEVNEEQGRALLEQKAIDLLIIDSQVIARED
                     DKSQFAVRALKQYMEAEELNRVGNSFSEADAFPLRVAINYLGAEQAPVQGEAGGTLPT
                     VKPEEVIIPSLTPPPAPFTQVLVALLYILPITFISIFFTSSFMDEKMNRRLTILLSAP
                     VSPFQIIFGKMLPYAIFALVSTALIAALTKTNIPLALAIFAPTTLFIFAIYLMVPLFY
                     RTFKDTTFIAMLVTTLTTAYLVFPAMFTNVNDLAYISPLTLAVKMYRGEPFGWREYLF
                     PSLPMAAIFGFAMFAGTRMLNEEFLMGYKPLTRKIKDAIYLMMAHAKPSIAVPLWSLI
                     VIPAVYMTQIVFLAFATNLPVGLILGFTLIASALVEEVVKTIGIVVLAERGVVKTWRE
                     VIGLSFLSALGFLIGEKLLLLVSISMVSEAQVSGALFGAGLLLFVPLAAHFVFVTIVT
                     VMAAKWRVPYWLALGVGTILHFIYNVNVMRGGM"
     gene            13211..14233
                     /locus_tag="PLE39_08380"
     CDS             13211..14233
                     /locus_tag="PLE39_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE68383.1"
                     /translation="MLAVAKKELMSVLRDRTIVISILIQLFIASFSSALLLGMLSLYD
                     SDTIMRFSGAGMRIGFVRAPVNVLEQLMGERGLTIVQFPSLGEAQAAYYRGEVLAIVE
                     PPQDANGLTETRIYLPESDTVNSLIRMVIQEPLKQYENHLRGQKGIEVRYADLKGKPS
                     TSYEFVYSVLIPMLMFFPAFVAGSMAVDSLTEEVENKTLQTLLSAPLSVNGMVAGKIL
                     ASVILSVAQCMAWLTLLQLNGIVIQNAGWILTLATIVAGITSTVAALGAVMLQDRERS
                     QFIYSLMLLAAAGISTLLNISPITTLSRLAIGDAYTNGWNVTVFAVFLAGLYFLLIRM
                     SRRLLA"
     gene            complement(14243..15247)
                     /locus_tag="PLE39_08385"
     CDS             complement(14243..15247)
                     /locus_tag="PLE39_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNE68384.1"
                     /translation="MSLQLWHNPEWQKQAHDWIRSEAGKNSIQLTGEIEQPHAYAWST
                     VMRVSSSAGMLFFKATAAETVYEIALTKKMAEWFPDSLPDIISADPARGWMLMRDGGE
                     QLRASIRPTKNIKPWEPVITRYADLQIGLASHVNEVLALGIPDHRLGTLPTLYKQLLK
                     DEDSLMIGKEKGLTETEFQQLKELQPRFENICSDLAAIQIPESVNHGDFHDGNILLKD
                     ERITFFDWGDASVTHPFVSLRTFFVSIEIALDLEDYSFTPAMAALLELYLKPWQAFAS
                     PASLQTAYRLSKPVASIVKTLSWHTTISRLEGSKRDEYAWIVPEVLREFMVYEKALQG
                     "
     gene            complement(15251..15988)
                     /locus_tag="PLE39_08390"
     CDS             complement(15251..15988)
                     /locus_tag="PLE39_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010081311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNE68385.1"
                     /translation="MQILGIDVGGSGIKGAPVDIETGQLAAERLRIKTPKKGEPEPIA
                     EVVKQIAQSFNWSGPIGIGFPAPIKSGVAMMAANVSDKWVGLNADELFSKVTECECTM
                     INDADAAGLAEMTIGAGRGQSGTVIMITLGTGIGTAIFHRGHLLPNTEFGHLEIKGKD
                     AEFRASAFARERDKLSWKKYAKRLNRYLSTMEKLFWPDLFIVGGGISKDSKKFLPLLT
                     IETPVVPAQLLNDAGIVGAAMGAAQKK"
     gene            complement(16083..17171)
                     /locus_tag="PLE39_08395"
     CDS             complement(16083..17171)
                     /locus_tag="PLE39_08395"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNE68386.1"
                     /translation="MQPSAIPTEKLLEFMEAAGVPGLAFSVIQNRQIISTQVIGIKNA
                     EIKDDPITEDTIFQAASLSKPVFTYGVLTLQQEGKLDLDKPLHDYLPLPEANEIPQLK
                     LITARQALTHTSGLQNWRFDIEDKFEFEFEPGTGFSYSGEGFFYVQRVIEQITGQSIE
                     SFLQDRVLRPFGMSNSTYIWRAEHEAMISMGHRDRGQKVNPWNAWQGRKLMEIAAQKN
                     KALDDWLYQDFVEALPQVHANLSPLPNNMIPNVAGSLLTTAPEYAQFMLRLLEPKNEI
                     AEQMLKSQPPLNSPLSWGIGIGLEKIGDRNCFWHWGENGCFENFMFGDPETGNGIVIL
                     TNGNRGLKICERIVQAVTGQSLNAFLWV"
     gene            complement(17211..18266)
                     /locus_tag="PLE39_08400"
     CDS             complement(17211..18266)
                     /locus_tag="PLE39_08400"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNE68387.1"
                     /translation="MENRKRARELGIAPGILPTGKLNAITDVAGMKVGQVTLIEGTDI
                     RTGVTAILPHAGNLFQEKVPAGVAVGNGFGKLMGSTQIMELGEIETPIILTNTLSVPR
                     AAEALILFALGQAGNGEVRSVNPIVGETNDGTLNNIRRLAITTEHVIQAIESAKDGQV
                     FEGNVGAGTGTICFGWKGGMGTSSRVLPQNLGGHTVGVLVQSNFGGVLQMDGIPVGKN
                     LGQYYLKDKLNDNSADGSIMIVVATDAPLSDRNLSRLARRGLSGLAKTGAAMSDGSGD
                     YVIAFSTAEEVRRTAERRGKIWAYPEVPNAGMSALFQAVIEATEEAIYNSLCMAETMT
                     GHEGRTIHALPLSKLVF"
     gene            complement(18268..18759)
                     /locus_tag="PLE39_08405"
     CDS             complement(18268..18759)
                     /locus_tag="PLE39_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68388.1"
                     /translation="MSKLKPVALMALLVFVLACQTLQPQPPTATAIPTPPPDLTVNTD
                     EEFVLVAGQSATINDSPLTLKLVGVTDDARCPSQIECAMSGPVSLTLIVTTDQGGRHE
                     VNLQTFTNAQGRAPEMEFEGIQSHVEIEGYSIRVMAVLPYPATLADRGKGVYQITLIV
                     KKV"
     gene            18928..19797
                     /locus_tag="PLE39_08410"
     CDS             18928..19797
                     /locus_tag="PLE39_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68389.1"
                     /translation="MQLNIQHLGKQYKRDFWGLKDFSLEIKPGILGLLGPNGAGKSTF
                     MRMLATITKPTEGSIKWNEVDVVKQPDALRDVLGYLPQDFGVYPNLTAVEFLEYMAAI
                     KGLDAVTAKKRIDELLQLTNLVQAAKRPLGGYSGGMKQRVGIAQALLNDPQLLIVDEP
                     TVGLDPEERVRFRNLLSDLSGERIVILSTHIVSDVEATATHIALVNKGQLLREAAPEK
                     LLQELEGKVWEWTIHSDDLPALKQKHIISGTIRRSDGVQVRVVSSTQPTAESRNASPN
                     LEDAYLFFIGGKQ"
     gene            19794..21356
                     /locus_tag="PLE39_08415"
     CDS             19794..21356
                     /locus_tag="PLE39_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNE68390.1"
                     /translation="MKSARIIYQLARADFLERVRRYSFLVMLGLVVFLGYQTAIGNMS
                     LELGAYRGEFNSAWVGAMMSLIGTFFIGWFGFYLVKGSVARDRETGVGQIMATTPLTR
                     PLYLLGKWLSNFAVLMSMVGVLALAGIAIQLLQGENTQIQLLEFLDPFVFIVMPLMAL
                     VAALAVLFESISFLQGGFGNIVYFFAFIMFLPLFMENDTLQKYPAFEPMGLGLLAGEM
                     GDAVRAIHPDYDGSFTLGGGIVEQATTTFVWNGIAWTPEYLFARFALIFFAVGLILFA
                     ALFFDRFDPSRSKPRKARSASSSEPTPASTPQAFPAPHLTPLNKSANRFSFFTVLIAE
                     LKLLIKGQRWWWYAISLGIIVACFVSPSKVVREIALPIAWIWPILIWSSIGNREVYNN
                     VQQLTFSSASPLWRQVPAQWLAGWIVTLLVSIGAISRFLMDGDTVGLLALLSGTIFIP
                     SLALASGVWSGTSKLFEILYMVIWYLGPLNNAPGLDFIGSHGNGRPEFFIPLSIALVA
                     FAFFGRARQLQQ"
     gene            complement(21366..21884)
                     /locus_tag="PLE39_08420"
     CDS             complement(21366..21884)
                     /locus_tag="PLE39_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68391.1"
                     /translation="MHTCPNCTNELSNIGAGAQFVQCAKCASILELADTVTLLTDRPV
                     STPIQWVAHCVLPDGTRFVSDGQILLAASYTGTAEPLPGKALPVETFQRLLNWNVKSN
                     FGIEHLLEDPARGNYYAPFDVLLNGKYIKYLTSLRYKQKLSFHVNAPLEPIVLVDGGK
                     KIGVLMPMKPPK"
     gene            complement(21933..23102)
                     /locus_tag="PLE39_08425"
     CDS             complement(21933..23102)
                     /locus_tag="PLE39_08425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE68392.1"
                     /translation="MTQTYDAIVIGAGVMGASIAFHLAERGLKTAILERKVTASGATG
                     HSSGLVRMHYDLAAESELTFTSYKNYFANWRERVGGECGFQKTGFLQVAKREHEDKLR
                     GNVANQQKIGINTSVISADEVKKLFPDLVTEHFDFAAYEPDSGYADATLTTNSFLDSA
                     KRNGAALIQNCEVTAIHTAGGKMTGVSTTKGDFAAPIIVNAAGPWAKQVAALAGVDVP
                     LVTWTHDVAFLHRPPSLGKFPAVIDDVINCYFRPEGSALILAAGEDESLRGEAPDAED
                     QTPTPTFVEKLIDAMVRRMPKIEESGLHSIHVGRDGITPDQRAIYSAAGPDGFYLACG
                     LSGTGFKTSPAAGASMVELILDGKPKTVDITPFRFQRFAEGKLIEGEYGYGHIWK"
     gene            23237..23626
                     /locus_tag="PLE39_08430"
     CDS             23237..23626
                     /locus_tag="PLE39_08430"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE68393.1"
                     /translation="MKKQSISQISEQIAAPLAAISTPQRIAILLAIGTGEACVCHLET
                     ALGWRQAYISQHLMALRKADILQDRREGRYVFYKLRNASFLDLITASATLSGLAAEAV
                     STLVNTRTYPACECPQCVPALIPVNSL"
     gene            23634..24674
                     /locus_tag="PLE39_08435"
     CDS             23634..24674
                     /locus_tag="PLE39_08435"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNE68394.1"
                     /translation="MTTLSSPSQSHKYLPSLVFFSAVIWLIAYNTIQPLADWISYTAL
                     GLARDSRLGESLAFFLYDVPKILLLLSGMVFLISIVRTFFSPERTRALLGGKREGVGN
                     IFAAMLGIVTPFCSCSAVPLFIGFVESGIPLGVTFSFLIAAPTINEVAVILLFGLFGW
                     KVAGLYVASGLVVAIVAGFIIGRLKMERYVADFVWKVQRKVDAFEEKLTWEDRIERAW
                     DSVKEIVGKVWLYVVVGIAIGAGIHGYVPEDAMASILGKQAWWSVPVAVLIGIPLYSN
                     AAGIVPIVGTLIEKGASVGTALAFMMSVIGLSLPEAVILKRVLKMPLLVTFFAVIAVA
                     IMITGYMFNLIL"
     gene            24731..24982
                     /locus_tag="PLE39_08440"
     CDS             24731..24982
                     /locus_tag="PLE39_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNE68395.1"
                     /translation="MLTIKILGSGCENCKRLAWLADRVVNHMGVQAQIVKVTDYAEIM
                     QYPIMSTPGLVINEKLVSSGRIPSETEISVFLTTALETA"
     gene            25014..26087
                     /gene="arsB"
                     /locus_tag="PLE39_08445"
     CDS             25014..26087
                     /gene="arsB"
                     /locus_tag="PLE39_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNE68396.1"
                     /translation="MKNETRKELSFLDRYLTLWIFLAMAVGVGIGFLFPKAVADFNAA
                     VSIGTTNIPIAIGLILMMYPPLAKVRYDELGDVFRNWKILSLSLVQNWVIGPVLMFVL
                     AIVFLRGYPEYMTGLILIGLARCIAMVIVWNELAKGDNEYAAGLVAFNSIFQVLFYSL
                     YAYVFITILPTWFGMTGNIVNITIGEIARSVFIYLGIPFIAGFFTRFFLVRAKGKDWY
                     HKVFIPKISPLTLVALLFTIVVMFSLKGDIIVQLPLDVVRIAIPLLIYFVIMFLVSFF
                     MGKSIGADYSRTTTLSFTAASNNFELAIAVAVAVFGINSGAAFAAVIGPLIEVPVMIA
                     LVNVAFFFQKRYFGGVVSSAPAR"
     gene            complement(26146..27285)
                     /locus_tag="PLE39_08450"
     CDS             complement(26146..27285)
                     /locus_tag="PLE39_08450"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNE68397.1"
                     /translation="MNNRSKELDGLRGIAILLVIAHHVLKRADYFTANGFLHFITRLS
                     LVGWMGVDIFFVLSGFLITSILLKTREEKNYYKNFYARRVLRIFPLYYTALALFLIAV
                     KFVPAVAETADSFIPYLPTFLLYQQNWLYLFAIYPSAYLSVTWSLAIEEQFYLIWPAI
                     VRIVNKHTLTWISAGIVILSLLIRTAAILLSPDSEQLPVVFFYNTFTRFEGLALGALT
                     AIFFVTPTNWQETAKRFALPVSIITMILFLYLAFIASPAGPHPLYRNIPLGTIGYTLL
                     AFSSAAMIVFLTSGAETSLIRKFFANGGLVFFGKYSYSMYLFHMPVALTLLDIMVKAK
                     VRGWEYYAAYSLLTVAITTVLALLTWNILEDPLLKLKRHFTNEPT"
     gene            complement(27282..27905)
                     /locus_tag="PLE39_08455"
     CDS             complement(27282..27905)
                     /locus_tag="PLE39_08455"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNE68398.1"
                     /translation="MTQPTPSAKQYSSLEELFSAMKRMNDDPLAKAGTNIVISRGNPK
                     ARLLIIGEAPGPEENIQGKPFVGRAGQMLDKILQAADFDPEKDVYITNSVFRMPPGEE
                     GKTFRKPTDAETDFYRPYVFEIIRLIDPLVMLLTGNVASYSILRKTGITSLRGKWTES
                     QGRWIMPIFHPSYLLRNPSKEPGSPKAQMWDDIREVRKKYDELIAAS"
     gene            complement(27979..29022)
                     /locus_tag="PLE39_08460"
     CDS             complement(27979..29022)
                     /locus_tag="PLE39_08460"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HNE68399.1"
                     /translation="MLNNPHRRYNALTGEWVLVSPHRAKRPWLGQVEKLSPETLPAYD
                     PTCYLCPRNERAGGVFNSDYAGTYVFNNDFASLLPDAPEEDASASSHPLLVSAPEQGQ
                     CLVVCFSPRHDLTLPELELPVIENVLNTWTNESARLAELDYIKYVQVFENKGAMMGCS
                     NPHPHSQLWSQSQLPNEIVKELATQTEYFKKNNSPLLVDYVKEEHKRKDRILFSNDHF
                     TALVPFWAVWPFEVLVSAHRASASLKELSAVEISALAEVFKQVTIRYDNLFEISFPYS
                     MGFHQAPADGQPHPEWTLHAHFYPPLLRSATVRKFMVGYEMLAMPQRDITPEVATERL
                     RSLSDVHYKHQKK"
     gene            complement(29025..>30030)
                     /locus_tag="PLE39_08465"
     CDS             complement(29025..>30030)
                     /locus_tag="PLE39_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68400.1"
                     /translation="DHQTSRYGIFDVTQPIVQKVLSERAKEFIRRYKPDLIKFDFGYE
                     LPSLDVAAPTDMNWAGERLLQKGLEVVVGAMKEENPNLVIMYYGLSPLLIEYYDLHSP
                     DDLVYCIGDYDLETNRRVFFSSLCGELGMPTYSSSGYEWESAPDIWFDTIASGTLGSL
                     HCFDGDESGGLPTDEVIAKFNGLAAIARKQVIFQTYPVHAKWQGGLRAGFSPSWERIE
                     NDKTVLLALRTHHLDGKPAPKHYKDILHTDVPLVVASLTDDGISESLRIGIVPFGDGS
                     CTISHTSLHNSTTIIEHYFSGRATKTQTACSNSQIEIKLQQTRNHEILEWIEIIFEKE
                     "
BASE COUNT         6496 a         7521 c         7989 g         7824 t
ORIGIN      
        1 aaaagaaact tgaattgaca tcccgccctg agtataaaaa aacagcctcc cgaaaatagg
       61 gaggctgttt ttgaaccaga caaagccgac tagccgccgc ttcccacggc attgatgatg
      121 atgcccaaaa cgataccagc gatccagacg tacattgggg tcttgagttt gagttcttca
      181 ttcttcatat tttgaagacc ccagataaag gtgtagagca tacaaaggaa gcccaaaatg
      241 cccttcatca caccttcttt ctggaacaac ttgatccaaa cccaaatgcc tgcaataaac
      301 gcaaggattc caccaagacc agtattcatt gaaaattcca tttcacactc tcctcttaaa
      361 gttgatggga tttatgcaat caggcgaaac gcccaataaa gcactaatga gaaaaacacc
      421 catgcagtaa ccccgttacg tgtttcaagg cggtttacga aactgataat tttttttctt
      481 gctggttcgg gcaatatcaa aactgaaatc atcaataaca gagcagcaag gaaaatcggc
      541 gcaaaagcgt gggtttgaag tgatgcagcg acctcaccgt gaaatagctg catggtagca
      601 cgggttaacc cgcagcccgg gcagggaacc cccgtagcgg caaggatggg gcaggtccaa
      661 aacgagattc ccgccaggct taaccccaaa tgcacaaccc caaacccgat tatggctgtg
      721 gtttcagtgc gattggttaa tagatttgaa aaaacaggtt gatgccagag tgttttgatt
      781 gaatgttcca tgagagtggt aacacatgga cacccaaaag gatgtcatga ccaaaaaaac
      841 acaaatcaag cagatgaacc catcacagcg aaaacattga tggcctgatt cttgcctttg
      901 aaaacgatct tccccaaatc ctccaccaga atatcggcag gcaggttttg gcgggtgtag
      961 ttatcgatca agatcgggtg cagggcttcc tttgtgtatt cttcaattct tgaggccaca
     1021 tttaccgtgt caccgatgca ggtgtaaatg gcacgcgttt gagtccccgt gtagcctgcc
     1081 accatctgac ctgtggcgat cccaatgccg atgcgaattt gggtcttgcc gtgagccgcc
     1141 tgattgtgat tgaaaacctt caactgttcc agcatttgca gggcggcgcg cacagccctt
     1201 tcagaatggt cttcaaaaaa gatcggcgca ccaaaaacgg ccatcaatcc atcgcccacc
     1261 acctgattga ccgtgccgcc gttgttcgtg atcgcatcga acatcaggct gaaataatga
     1321 ttaagcaggt caatggattc agatggctcc tgattttcag caatgctggt aaacgagcgg
     1381 atatcggcga acatgatgct ggcttttacc tgcttgcctc caagtgaaaa acctgaacgc
     1441 agcaattgtt cggccacttc cttggtggca aaagttctaa acaggcgtct ttgttcatca
     1501 cgcaggcgtt ttttttcgag gctggcattc acccgcgcac gcagcaagat cgggttgacg
     1561 ggtttcgtaa ggtaatcctc tgcaccgagt tcaacgcatt taaccacggc atccagctct
     1621 tccatcgctg aagtcatgat aatggggatc tgccgaaggt ccacatcgtt caaacaggcc
     1681 tcaagcacct caaacccgtt catctcgggc atctcaatat ccaacagcat caaatcgaat
     1741 gtgcccgtgc ggaatttatc caacccctcc ctgccgttct cagcggtttc cacaatatgc
     1801 ccctgctgct caaggctgcg cgaaagcacc atgcggttga ccttgttgtc atccacgatc
     1861 agcaaatggg cgggttttgg agattcagcc attggattta cctcattccc agcagttgac
     1921 ccctgacctg ttcaaacgca tcctgcagga cctcgagcct gttcccgatt tcgaggtttt
     1981 tttcacgccc gagcatttcc aactcgcgtg cgagtcccgc gagctcggtg gcaccaaatg
     2041 tcgccgcatt cgatttaagc gaatgtgccg cacgccgaaa tagatccaca tcctgtgcag
     2101 ccaggccgct cttcatttgc ccgatctgtg tgagggcatc ttccaaaaat gtatcgatca
     2161 tttcaccgat gaaatcggca cccaccgtct ctttcaaatt agtaaatgta tttaaatcaa
     2221 tgactgacat ttttctccat ttgtttgatt atcacattag aaagccttta cggcctgaag
     2281 ttagctgact cttcttgctt tcagtaacgc atccaccaat tccaccacgc ggatgggttt
     2341 tggaatgtag tggttcatgc ccgcaccaag gcacatttca cggtcgccct gcatggcatt
     2401 ggccgtcaac ccgatgatga agggacgttt atttacccag cgtgccacga tctgacgggt
     2461 ggcctcaaga ccgtccattt caggcatttg cacatccatc aagaccacgt catattgctg
     2521 gcgttcgatg gattgaatgg cttcaatgcc attcgaggcc acatcggcac gatagcccat
     2581 ttgctcaagc aggcgcagcg ccagcttttg gttgacagca ttatcttcag cgaggagaat
     2641 tctaagcgga tgccgcacag ccatttgcgg gtcgggtttg aagcggcctg tcgtcggcgg
     2701 ctcctcgtag gctgagggtc tgaaaaagag tccaaccaat gtatcaaaaa gctgtgattg
     2761 cttgatcggc ttggtaaggt aggcggcaaa gacgttggtt tcatcgcccg cttcacgccg
     2821 acccagtgaa ctgaaaagca ccagcgggaa ttttgccttt gcacgaatct ttgttgccag
     2881 ttcgaggccg tccatttcgg gcatatgcat atcgaggatg gcaacatcaa aggcttcacc
     2941 ctgttcgatc catttcagcg cggtgagggg cgaggcggtt tcgtgcggga tcatgcccca
     3001 tttggaagtt tgtgcgttga ggatgtagcg gttggtggaa ttgtcatcca caatgagtac
     3061 gtgcttgttt tgcagttcgg gttgaatgcc cgaatgtccg cgcttttggg cggggagaat
     3121 ttctgcgagt tcagctttga cggtaaagat gaacgtggag cctctgccca atccgtcact
     3181 ggtggcccac atggtgccgc ccatcatttc agcgagatgt ttgctgatgg ccaatcccaa
     3241 ccctgtgccg ccatacttgc gtgtggtgga tgaatcggcc tgtgaaaatg attgaaagag
     3301 tcggctcatg ccgtcttttg aaaggccgat gcctgtatcg cggacggaga atgtgagcag
     3361 ggctttttct tttgcgccgc cgttcctgta gcgctgactg gaaaccgtga gcaccacttc
     3421 acctttttct gtgaatttga ccgcattgct gagcagattg gtgatgatct ggcgcaggcg
     3481 ggttacatcg cccacaatgg cgggtggaat gtcgccttcg aaaatgtagg cgaggtcgat
     3541 ccctttttcg acggcccgag cagaaaccag gtccagtgat gattcgacac aatcgcgcag
     3601 gtcgaagggg tgatgttcga tatccattct ccctgcttcg atctttgaga aatccaaaat
     3661 gtcattgatg atggccagca gggcatcgcc tgaattgcgg atggtttcgg cataatcacg
     3721 ttgttcatcg tcgagcttgg tgtcaagcag gaggccgctc atgccgatga cggcattcat
     3781 tggggtgcgg atctcatgac tcatggtggc gaggaaggaa ctcttggcct cgttggcctg
     3841 ttcggcggcg gcgcgagcta ggcgggcatc ctcaaagagg cgtgcattct gaatggctgt
     3901 cgccatgctg gaggcgatgg attccatcag ggtgatgatg gattcatcgt aggcgttttc
     3961 gcgctcaaag ttttgaatgg tgattccacc gatgaggtct ttgccgacca ccatcggcac
     4021 catgatcacg gatttgggga cctcattgag cgccgtcatg ttggagccga ggcgcttcca
     4081 caatgaaggg gattggcggt tgataaccag cgatttaccc agcatggatg cgctgatcgt
     4141 ggaatcgttg atggggaatg agggcgtttg aatgcgttcg ttcttgcggg tgacgtaatg
     4201 tgctgtgatg gagcgcgcct cggggtcata taacccgatg cctacattgt cttccatcac
     4261 cagttcgcgg atcttatcgc cgacacgttc gacaatggtt tgcacatcca attgctgggt
     4321 gagcacctgt gcaatgttat tgaggatctc caattgggag gcgcggcgtt tggtttcctt
     4381 gagcaggcgc tgggtttcat cgaagaggcg cgcattttca atggtcacgc tcaatgagtt
     4441 ggcaatggtg gtgatcaggc gtacatcggc ttcgctgaag gcttcttcat gctcaaggtt
     4501 ctgcagagaa atgaccccgc gtacctcacc cgccaccgac attggcaccc acacacccga
     4561 acgcacaacc acatccccgg ggttggctcc cagcaaaacc gagttgtact tttgcgaaag
     4621 ttcgtcgaag ttgcggttgg ctacgatggg ctcacctgtg cggatcacat gtccgctgaa
     4681 cccgcccatg ccatctttca ataccagtgg ctcctgataa aaacgctcgc cgttctcgaa
     4741 aatgtacggg aattgcgtga tgttggtttc cttgtcgtac atggttagca caacggtttg
     4801 ggcatcgaat attttttgga tctggtcgcc gacatcgtcg tagatggttt ggatatcgag
     4861 tttggggtca agacctgaga tcagcgcgtt gatgatggaa agctcattgg cacgcttgtt
     4921 gatgaaatcg aacatgctgg cgttttgaat ggcaatggcc gcctgcaggg aaagccgttt
     4981 tagaaaatca aggtccgctt ctatgaaggg gtgaccgcca ttgcgccata cggccatcat
     5041 gccgccgacc ttgccgccgg tcagcagggg ggccgccatc aaccgttcct cagtctcaac
     5101 cggggtgcct ggaatctgta tggtgcgcgg gtcatgggcg gtatcgttga tgaattcacc
     5161 ctgccccgtt ttggcgagtc cgccgatgat gccctggccg aagtaaatgg tatccacctt
     5221 gatctcttcg gcgatctctc cttcggcggc aatggctttg aagtatgagc cgtcaccttg
     5281 cggtagataa atggcgctgg tggatgcgga gagcaggtca cgcgcatggt gggcgatctt
     5341 gtccatcacc gccttcagat caagcgtgga tgaaatatcg cgccctactt cattcagcac
     5401 cgaactttca cgtgcgcggc gctgggtttc atcgaaaaga cgggcattct caagtgcaat
     5461 ggccattgag ttggccaggg tttgtagcag acgcaggtct gattctttga aaacgttttc
     5521 cctatctaca ttttgcagtg aaatgacacc cttcacctga tttcccacca gcatgggcac
     5581 atccatgcgc gatttgatat cctctccgcc gccaaggatg ctggagcgcg tgccaagcag
     5641 gcgttcctct tcattggaca tatttgcgtt gatgataacg ggttggtggg tacgaagcac
     5701 ataccccgaa ataccgctgg ggggtaaagg ttcctggttg aggcgtttgc ccatttccat
     5761 taaataggga aaggagcaga tgttcttctc agcatcgtag gaaacgatca tcacgacctg
     5821 tacatcgaag atccctgcga tcttttcacc aaccaggctg tagagtgtat ctccatccag
     5881 ttgtgaagcc agtttggcct gcacactgtt gacgatctcc aactcagccc cacgctgggt
     5941 actttcctcc agcagccgct gcgactcgtt gaaaagacgc gagttttcaa tggcgatcga
     6001 agcctgatct gcgaataact gcaaaaggcg tagctcggta ttggcaaagg cacgcgcttc
     6061 agtgaagtgg atgttcatca cacccaccac acggtggcgg aatttgagcg gcaggccgat
     6121 caatgcaccc gtccaacgcg ctggcgaatc cttgtatagc ggatgcttac gcatgtcttc
     6181 caccaacatg atctcgcctg tctttgccac ggtgtcggtc attccaccgg gacggggtgc
     6241 tgaaacagga gtgcttttga tctcaccgtt ggcgatggcc gtgccaaata ccagttcatc
     6301 gttttcgtac aggaacaggt tgatgttttc aatgctcggc attaattcag atgtgctttt
     6361 caggatcgaa tccaacaccg tggaatattc aaggttcgac gaaaggtcaa tgctggcctt
     6421 tcgaattgct tcaagctcgg cattgtgctg ctcggtttcc ttgagcaggc gctgggtctc
     6481 gctcaacagg cgcgcatttt ccattgcggc actcatcgaa cttgccagag tctccaacag
     6541 gcttacatct gatggactga aggaattctc ccggtccatg ttttgcagtg aaatgacccc
     6601 gcatacttca ccgccgctga tcagcggaac gtacagggca gattttggaa tcaatggccc
     6661 cagcacgagc ttcgccccat acgcctccat ctgttctttg acatggtcgt tgaacaggaa
     6721 ggtttttttc aaccgtgcga tcgtttttgt aaaaccgctc atggcagtgg gttccaggtt
     6781 ttccctgcca aagcggtcaa tgtaataatt gaaatactgg gtctcggttt tcaggtcaaa
     6841 tgatgcaata agggtggttt gtgcgccaaa gatattatga atcttatccc ccacaagttc
     6901 atacacagcc tgcaattcca gctttgaagc cagaccatcc tgcacactct taatgatggc
     6961 cagctcagct tcacgctgct ttaactcctt gcgaatgcgc tcacttgaac ctgatcttga
     7021 agccgcagaa gttggtttct ttgccgtttt gggcgttgtt ttttttgctt tttcggtttt
     7081 aggcatgcgc acctctaaga cagaattcag tttacatacg ggcgggaaaa tcgtcaatag
     7141 cggaaataaa acaaacaggc gaatcggcct gtttgtcatt gttttgaaac ggattttgcg
     7201 cttcagactc agggttggcc tgcctctgtc acccaggcag gaatcggccc tgacggatca
     7261 aaaatattgg gcgtaagcct cacccagcag cttttccaga tacggcctga cctgccagaa
     7321 cgatttcatg ttgcggtagt cttccaccca gcgcggatcg ccaggttggg tggtcttcaa
     7381 agagcggatc atcagattct tgcccgtgtg ttcaatgggg acgaattgag ttacatcggt
     7441 gcgatagccc agcacacgca gcatggtggc acggaaggtg tctgtcagga tatctcccat
     7501 gcgttcgaac aaaatgccgt gatgaaaaac aggctgcatc tggctgggcg ttggcgcgtg
     7561 ggataactgc tcctgtaatt catgctggca gcatggcgca cagacgatga ccttgctttc
     7621 ccattggatt cccttggcga gcgcgtcgtc ggtggcggtg tcgcaggcgt gcagtgcaag
     7681 gatgacattc ggcttcactt caggttcata ctctgcgata taaccgacct gaaaagataa
     7741 gccgtcccac cccatggcgg cagcagtttg gttgtggcgc tcgatcaact cggcttttac
     7801 atccacgcct gtaacgcgag cgttgagatt taaaatattt cgcaggtaat aatagacggc
     7861 aaaggtgagg tgggcactgc cgcagccaaa gtccactacg ttgatgggtg ccccgctcca
     7921 ggcggagaaa gaagcggtct cttccagcaa tcgcaaaaat tcattgatct gtttatgctt
     7981 gcgctgcata tccgctttga tgcgcccatc cctggtcatg atgcccactg cctccaaaaa
     8041 tggacgcgcg ttttcatcgg agaggatgcg atgcttttca cggttgtgtg caaggttgac
     8101 cgtgagcggc actgcggcag gtttgaactc gttgacaatg ggtttgcctt ttttcgaaac
     8161 attgacctgc acattgcctg cattgctttc cacaaacatg ttgcggaagg gcagcgccag
     8221 caagtcttcc actttggctt tggcttgttt gaaagtgtag ttttgggtcg tgtctttttt
     8281 ttcgtcgaaa tacgagaact gcagttgaac ttcaccttga atttcaacag ggcgtacgat
     8341 gaccttttcc cactggatgg aaaacccctt ttgcccgccg ctaaatgtgg cgcggacgaa
     8401 ggtttcgcgg gcaaggatgc gttctcgaat cagttgcttg taatcttcca tgccgcgaag
     8461 tatatcattt gcgcgttaaa cagaatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnncgta cccgctccat taattcggcg ttggtttgtt tgcggttcgg gtcgccgtag
     8641 ccgtaatcat cggtataaat tttgagctgc tttattgcta tgccgccttg atacaggtca
     8701 actaaaaaat aaaaacggcc ataacgcgcc cattcccaat ctatgaattt tgctgtccac
     8761 gaaagtccgt ttccaagatc ggtcggttgg ggaaaatcct gcatgtctcc tacacttccc
     8821 tttcctcttc ctcttttgca gcctcgcccc gttccatttt tttcttcaaa tgctcgggcc
     8881 acgcgctgac attttctgac tgcaagaaca cagggctggt tcctgtgtgg gtcaaaatat
     8941 cccgtgcgcg gcaattgagc acggcttttg ccgcatcaat gcctgattgc gtcacccctg
     9001 ccaccccgtg actgatggtg ctttgcccgc acagatacaa gccttcgaat tcggtgcgcg
     9061 gtgtaaaccc aagcggaccc acttgcgatg gacgtttatc aatgccgtat aaatttccgc
     9121 gggtggcgtt gaggtagtgt tcattcgtca atggagtgcc gaggctgtag aaggtgatgt
     9181 gcttgctcaa gccggggatg cgtttctcaa gtccgcgcac catgcgccag gcgaggtctt
     9241 ctttcatggc ttcgtaatcg gcagggcgtg cgccgtattt tgaattcgcc catttctcaa
     9301 aggcatcgta tcccacaaac gcaaacgact cgcaagtgtg ttgtccgtga tgcatcttcg
     9361 aggggtcttt caacgtcgtt gtggtcagga acatgccggg cggagtctca tcattcaaca
     9421 cggcatcggt gaggccttgg gtgtagatct tgtccacgtc ggcatggtcg taataccaca
     9481 tgttgcccga gtcgaggccc gcgccgcgca agtccatgtc ggtggcgaag aaaaggctga
     9541 ggcaggatgt tgaatattgg acagaatcaa ttttccgttt gagtcgagcc gagaggtttt
     9601 ccttgccgat caattttcca aaggtcactt ccgggtcggc gttcgagatg acgacatctg
     9661 cgcggatctc ttcacctgat tccaattcca cgcccagaac ttttttccct tcgaggagaa
     9721 tccgcttcac cctgcactga agcctgatct cacccccaga ctttttcagc gcccgaacaa
     9781 atgcgcgcgg aatggtgaac gcgccgccga gcggatagaa gccgccgtca agatagtggt
     9841 gggtgatgcc agcatggatg aacgccgaga cctgcgaagg cggcatgcca tgatcgcccg
     9901 attgcgccgc aagtacgccg cgcaacacgg ggtcgctgat gtgtgcgttg atcaaatccg
     9961 cgcctgtgga acggatccat ttcaggatgt ttcccgcgct ttttgcggtt tcaagcgggt
    10021 tgctcaaatt gccgatgcgg cgcaggcctt ccatcatatt ggtgagatcg gtgaaatatg
    10081 agtcgatccc ctttgcctca tgcggaaaat ggctcttgag ccgctcgatt aaattttctt
    10141 tacctttggg aaaatcaatg cgtttttcgc cgatgaagat atggtcatat ccatcggggt
    10201 tgagttctac gaaggtcagg tcttgtgaaa cgcccagtcc gtcatagatg cggcgcaacc
    10261 cgccgccttc acccagtgaa ccaatgtaat gcacacccgg actgaagcga tagccttcaa
    10321 gcgtgaatga atgagtccag ccgccggggc gatcatgctg ctcgaggacg atcacttttt
    10381 tacccgcctg cgccaatgca acggcggcgg tcaacccacc tgcacccgag ccaattacga
    10441 tgacatcata cgtggacatg ttgcctccag annnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn nttcactcat tcgtcatcaa cttgacgtaa atttcctcca gcgccgattc
    10621 gcgcatggag aggtccatca gggtccattt gttggcggag acggtttcga gcatgtgggc
    10681 gattccatcc acctcggcag tgcggaagat gtaattgccg ttgacttctt cgtaatccag
    10741 tttttggtcg gcgtgaaaga tcacctgata ttcgcgcttc ccgagcctgg cacggatggc
    10801 ttccattgtg tcgaagatga gcagtttgcc gtttttgatg atgcccaccc ggtcgcagat
    10861 ggtttcgacg tgaaagaggt tgtgcgcgct gagcaaaatg gttttgcctt cgcggcgcag
    10921 ggttttgagg taattgatga tgaagaacga ggtcaatggg tcgaggcctg aattgggctc
    10981 gtccagaatc agcaggtcgg ggtcatggag cagggcgcgg gcaatggcgg tcttgcgttt
    11041 catgcccttg gagaattctc ccgtcagctt tttggtttcg tgcaggccca gagagttgag
    11101 caggtcgtca atacgcttca atgccttttg gcgcggcatt tgatacagtt cggagaaaaa
    11161 cagcaggtat tgctgggcgg tcatggcctc atacagcgga ctttcctcag gcaggtagcc
    11221 gatcttttgc ttgatcctgg cattgccttt ttgaatatct tcccccatca cacgcaccac
    11281 gcccgaggtc ggctcgatca gccctgcgat cattttcatg gtggtgcttt tccccgcacc
    11341 gttatggccg atgatgccaa cgatctcgcc cggatgaatt tgtaagttca actcgtggac
    11401 gactgtagta ttatcgtagc gcttggtgac attttctatg gttatcatgt tgtaatttta
    11461 tcctattatg aataatgtca ttgcgagggc gatagcccga agcaatctcc gacagtgaaa
    11521 gagattgctt cgggcaaaga acaagagcgc cctcgcaatg acataacgag agtcttatga
    11581 atcaaattct tgtcattgcc tggcgtgaga tcacacggtt gcgtaaacgc tttggcggcg
    11641 gggcgagtcc gctggcggtg ctggctttgc tggcagtgtt gggcgggtca tcttatttcc
    11701 tgcgcgatgc ggtttcgctt ggcagcggcc tgtaccgtgt gggcgtgatg ggcaatgtac
    11761 ccacgatcaa cgacagccgc tttgccgtga tggaagtgaa tgaggagcag gggagagccc
    11821 tgctcgaaca aaaagccatt gacctgctga tcattgattc gcaggtgatc gcgcgtgaag
    11881 atgacaagtc acagtttgcg gtgcgggcgt tgaaacaata catggaggcc gaggaattga
    11941 accgtgtggg gaattccttc tctgaagctg acgcttttcc actgcgggtg gcgatcaact
    12001 acctcggagc cgagcaggcc ccggtgcagg gtgaggcggg cggaacgctt cccacggtaa
    12061 agcctgaaga ggtcatcatc ccttcgctaa cgccgccgcc tgcgccgttt acgcaggtgc
    12121 tggttgccct gctttatatt ttgccgatca ccttcatcag cattttcttc acgagcagtt
    12181 ttatggatga gaaaatgaat cgccgcctga cgatcttgct ttcggcgcct gtttcgccgt
    12241 ttcagatcat cttcggcaag atgctgcctt atgcgatttt tgcactcgtt tccaccgcgc
    12301 tgatcgctgc tctgaccaaa acgaacattc ccctcgccct ggccattttc gcgccgacca
    12361 cactttttat ctttgcgatc tatttgatgg tgccgttgtt ctaccgcact ttcaaagata
    12421 caaccttcat tgccatgctg gtgacaacat tgacgacggc ataccttgtg tttcctgcga
    12481 tgttcacaaa cgtgaatgac cttgcctaca tttcgccttt gaccctggcg gtgaagatgt
    12541 atcgtggcga gccgtttggc tggcgcgagt atctttttcc atccctgccg atggcggcga
    12601 tctttggctt tgcgatgttc gcagggacgc gcatgttgaa cgaggaattt ttgatgggct
    12661 acaagcccct cacacgcaag atcaaggatg cgatctattt gatgatggcc cacgcgaaac
    12721 cgtccattgc tgtgccgttg tggagtttga tcgtgatccc tgctgtgtat atgacgcaga
    12781 tcgtttttct ggcattcgcc accaacctgc ctgtggggct gatcctcggt tttacattga
    12841 tcgcttcggc gctggtggag gaggtggtga agacgatcgg cattgtggtg ctggcggagc
    12901 ggggtgtggt gaaaacatgg cgcgaggtca ttggtttgtc gtttttatcg gcgctgggat
    12961 ttttgattgg tgaaaagctt ttgcttttgg tttcgatcag catggtttcg gaggcgcagg
    13021 tttcaggcgc gttgtttggt gcgggtttgt tgttgttcgt gcctttggcg gcacattttg
    13081 tgttcgtcac cattgttacc gtgatggcgg cgaagtggag ggttccttac tggctggctc
    13141 tgggagtggg gacgatcctg cattttattt acaatgtgaa tgtgatgcga ggtggaatgt
    13201 gattgctccg atgttggcgg tggctaaaaa ggaactcatg tcggtccttc gcgatcgtac
    13261 gatcgtgatc tcgatcttga tccaattgtt catcgcttca ttttcttctg cgttgttgtt
    13321 ggggatgctt tccctgtatg attccgatac gatcatgcgt ttcagcggag cagggatgag
    13381 gatcggcttt gtgcgtgcgc cagttaatgt gttggaacag ttgatgggtg aacgcggtct
    13441 gaccattgtt caattcccat ccctggggga ggcgcaggcc gcatattacc gtggtgaggt
    13501 gctcgcgatt gtggaacctc cacaggatgc gaacggcctg accgaaacaa ggatctacct
    13561 gcctgagtca gatacggtca attcactgat ccgcatggtg attcaggagc cgctcaaaca
    13621 atatgaaaac cacctgcgcg gacagaaggg gatcgaggtg cgctatgccg atttgaaggg
    13681 caaaccctca acctcgtatg aatttgtgta ttcggttttg attcccatgc tgatgttctt
    13741 ccctgctttc gttgcaggca gcatggccgt ggattcgctc acggaagaag tggaaaataa
    13801 gaccttgcaa accctgctct ctgcgccgtt gagtgtcaat ggcatggttg cgggcaaaat
    13861 tctggcttcg gtgattcttt cggtggcgca atgtatggcg tggctgaccc tgcttcagtt
    13921 aaatgggatc gtgattcaaa atgcaggctg gattttgacc ctggcaacca tcgtggcggg
    13981 catcacatcc actgttgcgg ctttgggtgc ggtgatgctg caagaccgag agcgttcaca
    14041 attcatttat tcgctcatgc tgctggcggc ggcgggtatc agcaccttgt taaatatttc
    14101 gccgatcacc acgctttcgc ggctggcaat cggcgatgcc tacacaaatg gctggaatgt
    14161 gacggtcttt gcagtgtttc tggcggggtt gtattttctt ttgatcagga tgtcgcggag
    14221 gctgctggct tgatctctgc ggtcagcctt gcaatgcttt ttcatacacc atgaattccc
    14281 gcaatacttc gggtacgatc catgcatatt catcgcgctt ggaaccttcc aaccgtgaaa
    14341 tggtggtgtg ccacgaaagt gttttgacaa tggatgccac tggttttgaa aggcggtagg
    14401 cggtttgcag gctggctggc gaggcgaaag cctgccaggg ttttaggtag agttcgagca
    14461 aggcggccat tgcgggggtg aaggaataat cttcgaggtc gagggcgatc tcaatgctga
    14521 cgaaaaaggt gcggagcgaa acgaatggat gggttacgct ggcatcgccc caatcgaaaa
    14581 aggtgatgcg ctcatctttc agcaggatgt taccgtcgtg aaaatcacca tgattgaccg
    14641 attcaggaat ttgaattgcg gcaaggtcag agcagatgtt ttcaaagcgt ggctgcaatt
    14701 ccttcagttg ttgaaactcg gtttcggtca gtcccttttc tttgccgatc atcaaactgt
    14761 cttcgtcttt gagtaactgt ttatacaggg tgggcagtgt acccaggcgg tgatcgggga
    14821 tcccgagcgc aaggacttca tttacatgac tggcaagccc gatctgcaga tcagcgtaac
    14881 gggtgatgac aggttcccag ggcttgatgt tttttgtcgg gcggatggaa gcgcgcagtt
    14941 gttcgccgcc atcgcgcatc aacatccaac cgcgtgcggg gtctgcactt atgatgtcgg
    15001 gcagggaatc ggggaaccac tccgccattt tttttgtcaa tgcaatttca taaaccgttt
    15061 cggcagcggt ggctttgaag aagagcatcc ctgcgcttga tgaaacgcgc atcacagtgg
    15121 accatgcata ggcatggggc tgctcgatct cacctgtcaa ctggatcgaa ttttttcctg
    15181 cttcagaccg tatccagtcg tgggcttgtt tttgccattc ggggttgtgc cagagttgca
    15241 ggctcatttt ttacttcttt tgcgcggctc ccatggccgc gccgacaatg ccagcatcgt
    15301 tcaataattg agcggggacg acgggggttt caatggtgag caaaggcagg aattttttag
    15361 agtctttgct gatgccgccg ccaacaatga acagatcagg ccagaaaagt ttttccattg
    15421 tgctcaagta tctgttcaac cgctttgcgt attttttcca tgaaagtttg tcgcgttcac
    15481 gggcaaatgc ggaggcgcga aactcggcat ccttgccttt gatctcaagg tggccaaact
    15541 ctgtattggg gagcagatgt ccgcggtgaa agatggcggt gccgattccc gttcccagtg
    15601 tgatcatgat aacggtccca gattgaccgc gccccgctcc gatggtcatc tctgccaggc
    15661 ctgccgcatc tgcgtcattg atcattgtgc attcacattc tgtcactttg gaaaaaagtt
    15721 catctgcgtt gaggccaacc catttatctg atacattggc cgccatcatc gccacaccgc
    15781 ttttgatcgg ggcagggaaa ccgatgccga tcggcccgct ccagttgaag gattgcgcga
    15841 tctgtttgac cacttctgca atgggttcgg gctccccttt tttgggagtc ttgatgcgaa
    15901 gccgttccgc cgctaattgc cccgtttcaa tgtccactgg tgcgcctttg atacctgagc
    15961 cgcctacgtc aattccgagg atttgcatga tctgttcctg cctttaagtt tatagtgggg
    16021 agatttaatc acatatgtct gacaaagaca atgtttaatt taaagctttc attttcttct
    16081 tcttacaccc atagaaaagc attgaggctt tgcccggtca cggcttgcac gatccgctcg
    16141 cagattttca ggcccctatt gccgttggtc agaatgacga tcccattccc tgtttcaggg
    16201 tctccgaaca tgaagttttc aaaacagcca ttctcgcccc aatgccaaaa acagtttcta
    16261 tcgccgatct tttcaagccc gatcccgatt ccccatgaga gcggactgtt caatggcggt
    16321 tgagatttga gcatttgctc cgcgatttca tttttgggtt caagcaaacg cagcataaat
    16381 tgagcgtatt cgggcgcagt ggtcaacaaa ctgcctgcta cgttgggaat catgttgttg
    16441 ggcaggggcg atagatttgc atgaacctgc ggcagggctt cgacaaaatc ctggtacaac
    16501 cagtcatcca acgccttgtt cttttgcgcg gcgatttcca ttaatttcct gccctgccat
    16561 gcattccacg gattgacttt ttgtccgcgg tcacgatgcc ccatgctgat catcgcctcg
    16621 tgctccgctc tccagatata agtgctattg ctcatcccaa atgggcgcaa aacccgatcc
    16681 tgtaaaaatg attcaatgct ttggccagtg atctgttcga tgacacgttg tacatagaag
    16741 aaaccttcac ctgaatagga gaagcctgtc cctggctcga attcaaactc aaatttgtct
    16801 tcgatatcaa aacgccaatt ctgcaggcct gatgtatgtg tcagcgcctg acgggctgtg
    16861 atcagtttta gttgggggat ctcgttcgct tcaggcaggg gaagataatc gtggagaggt
    16921 ttgtccaggt ccagctttcc ctcttgctgc aaggttagaa ctccataggt aaatactggt
    16981 ttgctcaaag acgcggcctg aaaaatagtg tcttcggtga tcgggtcgtc ttttatttct
    17041 gcattcttta tgccaataac ctgagtggat attatctgtc ggttttgaat cacactaaat
    17101 gccaggcctg gcacaccggc ggcttccatg aattccaaga gcttttcagt tggtatggct
    17161 gatggctgca tgtttgcttc tcctgataaa gtttatcaac acctgtccag ctaaaatacc
    17221 agcttactta agggtaatgc atgaattgta cgcccttcgt gtcctgtcat ggtctctgcc
    17281 atgcacaatg aattatagat ggcttcttcg gtggcttcaa taacggcctg gaacaatgcc
    17341 gacatccctg cattgggaac ttcgggatat gcccaaattt tcccgcgccg ctccgcggtt
    17401 ctgcgtactt cctccgctgt ggaaaatgca atgacataat ctcccgagcc atcgctcatg
    17461 gctgcgcctg tctttgccaa tccgcttagt ccacgccgtg ccagccgtga gaggtttcta
    17521 tctgaaagcg gcgcatcggt ggccacgacg atcatgatcg aaccatcagc actgttgtca
    17581 ttgagtttgt cttttaaata atattggccg aggtttttac caacggggat gccgtccatt
    17641 tgcagcacac cgccaaaatt tgattgcacc aacacaccta ctgtatgccc gccaaggttt
    17701 tgaggtaaca cccgcgaact cgtgcccatg ccgcccttcc acccaaaaca gatcgtcccc
    17761 gtacccgcac cgacgttgcc ctcaaaaact tgtccatctt ttgcgctttc gattgcctga
    17821 atgacgtgtt ccgttgtaat tgccagcctg cggatgttat tcaacgtccc atcattggtc
    17881 tctcccacaa taggattgac gcttcgcact tcaccgttgc ctgcctgccc caacgcaaac
    17941 aggatcagcg cctcagccgc gcgcggcaca gacagcgtgt tggtcaaaat gatgggcgtt
    18001 tcgatctcgc caagttccat gatttgcgtg gaccccatca acttgccgaa accatttccc
    18061 accgccaccc ccgcagggac tttctcttga aacagattcc cagcatgcgg caggattgca
    18121 gtcacacctg tgcgaatatc tgtcccttca atgagggtga cttgtcccac tttcatgcct
    18181 gctacatcgg tgatggcgtt cagctttccc gttggcaaaa tgcctggggc gatccccaat
    18241 tcccgtgcgc gtttgcggtt ttccattcta aaccttcttc acgatcaatg tgatttgata
    18301 cacacctttg cctcggtctg ccagtgtggc ggggtagggc agtacggcca tcacccgaat
    18361 tgaatatcct tcgatttcca catggctttg gatcccttcg aattccattt cgggtgcgcg
    18421 gccttgtgca ttggtaaaag tttgcaaatt gacttcatgc cgcccgccct ggtctgttgt
    18481 gacgatcagc gtgagtgaca caggtccgct catggcgcat tcgatctggc ttgggcagcg
    18541 ggcatcatcc gtcacgccga ccagtttcag ggtcaggggt gaatcgttga tcgtggctga
    18601 ttgtcccgca acgaggacga attcctcatc tgtatttacg gtcaggtctg gcgggggagt
    18661 cgggattgcg gttgcagttg gcggctgggg ttgcagggtt tggcaggcca gtacaaatac
    18721 caacaaagcc atcagagcca caggtttgag tttggacatg ggaacctcca ttgagtatga
    18781 cgattatata tcgccgattt tgtcagggac ggatggccag gcacacaggt tccagaatca
    18841 gtaccagagt acctgttgac ctagtggaaa atcactagta ttatttactt atcaatagta
    18901 aaaacctgat actacaaaga ggacgttatg caattgaaca ttcaacatct tggcaaacaa
    18961 tataaacgtg atttctgggg cttgaaagac ttctcgctgg agatcaaacc aggcatttta
    19021 ggcctgctcg gcccgaacgg tgcgggcaaa tccaccttta tgcggatgct ggcaaccatt
    19081 accaagccga ccgaaggcag cattaaatgg aacgaggtgg atgtcgtcaa acaacctgat
    19141 gcattgcgcg atgtgcttgg atatttgccg caggatttcg gcgtgtaccc aaatttgacc
    19201 gcagtggaat tccttgaata catggccgcc attaaggggc ttgatgcggt cactgcgaag
    19261 aaacgcattg atgaattgct gcaattgacc aaccttgtgc aggccgcgaa acgtccgctg
    19321 ggcggatatt cgggcggtat gaaacagcgc gtgggaatcg cacaggcctt gctcaatgat
    19381 ccgcaattgc tgattgtgga tgaacccact gtggggttgg atcctgaaga gagggtccgc
    19441 ttcaggaatt tgctctcaga cctgtcggga gaaaggatcg tgatcctgtc cacgcacatc
    19501 gtttcagatg tggaagcgac cgccacacac atcgcactcg tcaacaaagg tcaactgttg
    19561 cgtgaggctg cgcctgaaaa actgttgcag gagcttgaag gcaaggtttg ggagtggacg
    19621 attcacagcg atgacctgcc cgcattgaaa caaaagcaca tcatcagcgg tacgatccgc
    19681 agaagcgacg gggtacaggt gcgtgttgta agcagcaccc agcccacagc ggagtctaga
    19741 aacgcgagtc cgaatcttga agacgcatat ttgtttttca tcggcggtaa acaatgaaat
    19801 cagcacggat catttatcaa cttgcccgcg ccgattttct ggagcgtgtg cgccgttata
    19861 gttttcttgt gatgttgggg ctggtggttt ttctcggtta tcaaaccgcc atcggcaata
    19921 tgtctcttga gttgggagcg tatcgcggtg agttcaattc ggcctgggtc ggcgcgatga
    19981 tgtcgctgat cggcacattt ttcatcggct ggtttggctt ctatctcgtc aaaggctcgg
    20041 tggcccgtga ccgcgaaacg ggcgtcggcc agatcatggc gaccacaccg ctcacgcgtc
    20101 cgctgtattt gctcggtaaa tggcttagca acttcgccgt gttgatgtcg atggtcggtg
    20161 tactggcgct ggctggcatc gccattcaac tgctgcaagg tgaaaatacg cagatccaat
    20221 tgctggaatt tcttgaccca tttgttttta tcgtcatgcc gctcatggcg ctggtggctg
    20281 cgctggccgt gttattcgag tcgatctcgt tcttgcaagg cggctttggc aacatcgttt
    20341 actttttcgc attcatcatg ttcctgccgc tcttcatgga aaacgacacg ttgcaaaaat
    20401 accccgcctt cgaaccgatg ggccttggcc tgctggcagg cgaaatgggt gatgctgtcc
    20461 gtgccattca tcccgattac gatggcagtt tcaccctcgg cggcggcatt gtggaacagg
    20521 ccaccaccac ctttgtctgg aatggcattg cctggacccc cgaatatctt tttgcgcgtt
    20581 tcgctttgat cttctttgcc gttggcttga ttctctttgc tgcgcttttc ttcgaccgct
    20641 tcgacccttc tcgctcgaaa ccgcgcaaag cgcggtccgc ttcatcctcc gagccgacac
    20701 ctgcttcaac acctcaggca ttccctgcgc cgcatctgac tccgctcaac aagtcggcga
    20761 accgcttcag ctttttcacc gttctcatcg cagaacttaa actcctgatc aaaggtcaac
    20821 gctggtggtg gtatgcaatt tccctgggaa tcatcgttgc ttgttttgtg agtccatcca
    20881 aagtcgtgcg tgagatcgcc ctacccatcg catggatctg gcccattttg atctggtctt
    20941 ccattggcaa ccgcgaggtg tacaacaatg tccagcagtt gaccttctct tcggcctccc
    21001 cgttgtggcg gcaggtccct gctcaatggc tggctgggtg gatcgttacc ctgcttgtca
    21061 gcattggtgc catctcccgc ttcctcatgg atggcgatac cgtcggtttg cttgccctgc
    21121 tttcaggcac gatcttcatc ccatccctcg cccttgcctc aggcgtgtgg agcggaacga
    21181 gcaaactctt cgaaattctc tacatggtca tctggtatct tggcccgctt aacaacgccc
    21241 ccggcctgga ctttatcggt tcacatggca atggccgtcc tgaatttttc attccgcttt
    21301 caattgcgct ggttgccttt gccttcttcg gcagggcaag gcagttgcag caataatgca
    21361 gtcccttatt tcggcggctt cattggcatc agaaccccga tctttttgcc cccatcaacc
    21421 aacactatcg gctcaagcgg agcattgaca tggaaggaca atttctgctt gtaacgcagc
    21481 gaggtcaggt atttgatgta tttgccgttg agcagcacgt caaagggcgc gtagtaattt
    21541 ccacgcgctg ggtcttccag taaatgctca attccgaaat tcgatttgac attccagttc
    21601 aacaagcgtt gaaaggtttc aacaggcaga gccttgccgg gcaaaggctc tgccgttcct
    21661 gtgtaggatg cagccagcaa gatttggccg tcacttacga agcgtgttcc atcaggcagg
    21721 acacaatgag ccacccactg aattggggtt gacacgggcc tgtcggtgag cagggttacg
    21781 gtatctgcaa gttcaaggat gctggcacac ttggcgcact ggacgaactg cgccccggca
    21841 ccgatattgg aaagttcgtt tgtacaattg ggacaggtat gcatgaggac gctccttttg
    21901 tgggtgggga agtaagtggg gcgaatgttt tcctatttcc aaatatgccc gtacccatat
    21961 tcgccttcga tcaacttccc ttcagcaaat cgttgaaagc ggaacggggt gatatccaca
    22021 gtttttggct ttccatccag aatcaattcc accatgcttg cgcccgcggc gggggaggtc
    22081 ttgaagcctg tgccgctcaa cccgcaggcg agataaaaac catcaggtcc tgctgcgctg
    22141 taaatggcac gctgatcggg ggtgatgcca tcgcgcccca catgaatgga atgtaacccg
    22201 ctctcttcaa ttttcggcat ccgacgcacc attgcatcaa tgagtttttc cacaaaagtg
    22261 ggtgtgggag tctggtcttc ggcatcgggt gcttcgccgc gcagagattc atcctcaccc
    22321 gcagcaagga tcagcgcact gccctcagga cggaaatagc aattgatcac atcgtcgatc
    22381 accgcaggga atttacccaa tgaaggcggg cgatgcagga atgccacatc atgagtccat
    22441 gtcaccagcg gcacatccac gcctgccagc gccgcgacct gcttcgccca cggccccgcc
    22501 gcattgacga tgatcggcgc agcgaaatcg cctttcgtag tgctgactcc cgtcattttt
    22561 ccgcctgctg tatggatggc tgtcacttcg cagttttgaa tcagggccgc gccgtttcgt
    22621 ttcgcagaat caagaaacga gttggtcgtg agcgtcgcgt cggcgtatcc tgaatcgggt
    22681 tcgtaggcgg cgaaatcaaa gtgttctgtc acgagatcgg ggaaaagttt cttcacttca
    22741 tccgctgaaa tcaccgatgt gttgattcca attttctgct ggttggcaac attgccgcgc
    22801 agtttatctt catgttcacg tttggcaacc tgtaaaaagc cagtcttctg aaacccacat
    22861 tccccgccca cacgttcacg ccaattggcg aaataatttt tataactggt gaaagtcagt
    22921 tctgattccg cggcgagatc gtaatgcata cgcaccaatc cactggagtg accagtggcg
    22981 cccgaagcag tgactttgcg ttcaaggatg gcggtcttga gtccgcgctc ggcgaggtgg
    23041 aaggcaatgc ttgcgcccat taccccggcg ccgataacaa tggcatcata ggtctgtgtc
    23101 atgtgaggtt ccttttgcat tgggattgcc gcccattata taacctttgg ctcgggatgt
    23161 ctggcgcgat aatttatgac aaacattaca ttcgtaaacc tgaatgtgtt gctacaatat
    23221 attcaggttt atgaatatga aaaaacaatc catttctcaa atttccgaac agatcgccgc
    23281 gccgctggct gccatttcca caccacagcg cattgccatt ctgcttgcca tcggcaccgg
    23341 cgaagcctgc gtctgtcacc ttgaaaccgc gctgggatgg cggcaggctt acatttccca
    23401 gcatctgatg gcccttcgca aagccgatat tttgcaggat agacgcgaag gccgttacgt
    23461 tttttataaa ttaagaaacg cctcttttct ggacctgatc actgcttcag caaccctaag
    23521 cggactcgcc gccgaggcag tttccacgct cgtcaatacc cgcacgtatc cagcatgtga
    23581 atgcccgcaa tgtgtccctg cgttaatccc agtaaatagt ttgtaaggaa acaatgacca
    23641 ctctttcatc cccatcgcaa tctcacaagt atctcccgtc ccttgtgttt ttctctgctg
    23701 tgatctggtt gatcgcctac aacaccattc aacccctcgc agattggatc tcttacacag
    23761 ccctcggact ggcgcgcgat tcgcgccttg gcgaatcact ggctttcttt ttgtacgatg
    23821 tgccaaagat tttgctcctg ctttcgggca tggtcttcct catttcaatt gtccgcactt
    23881 ttttcagccc cgaacgcacc cgcgccctgc ttggcggaaa acgtgaaggg gttggcaaca
    23941 tctttgccgc catgctgggc attgtcacac ctttttgttc atgctctgcg gtgccgctct
    24001 ttatcgggtt tgtcgaatca ggcattccgt tgggtgtgac cttttccttt cttatcgctg
    24061 cacccacgat caatgaagtc gctgtgatct tgttattcgg tctctttggt tggaaggtgg
    24121 ctgggttgta tgttgcatct gggcttgtcg ttgccattgt cgctggcttc atcatcgggc
    24181 ggctcaaaat ggaaagatat gtcgccgatt ttgtctggaa ggtccagcgc aaagtggatg
    24241 cctttgaaga aaagttgacc tgggaagatc gcatcgaacg cgcctgggac tcggtgaagg
    24301 aaattgtcgg caaggtctgg ttatatgtag tggttggcat cgccatcggt gcagggattc
    24361 atggctacgt gcccgaagat gcgatggctt ccattttggg caaacaggcc tggtggtcgg
    24421 tgcccgtggc ggttctaatc ggcatcccgt tgtattccaa cgcggcgggt attgtcccca
    24481 ttgtgggcac cctgatcgaa aaaggcgcat cggttggaac tgcactggcg ttcatgatgt
    24541 cggtcatcgg gttgagcctg cctgaagccg tgatcctaaa gcgcgtgctc aaaatgccgt
    24601 tgcttgtgac gttcttcgct gtgatcgcag tggcgatcat gatcacggga tatatgttca
    24661 atttgatttt gtaaatgaca ggccccaggg tttctgaaaa cctctggggt ctttatataa
    24721 aggagcaaaa atgttaacca tcaaaatcct aggttcgggc tgtgagaatt gcaaacgcct
    24781 cgcatggctt gccgaccgtg tggtcaacca catgggtgtg caggcgcaga ttgtcaaagt
    24841 cactgactat gccgagatca tgcagtatcc catcatgtcc accccggggc tggtcatcaa
    24901 cgaaaaattg gtctcttcgg ggcgtatccc cagcgaaaca gagatctccg tttttctgac
    24961 gaccgctctt gaaaccgcat aaatgtttct tgcaaattta atatggaggc aaaatgaaaa
    25021 acgaaacgcg caaagaactt tcctttcttg accgctattt gaccttgtgg atattccttg
    25081 ccatggcagt cggtgtaggc atcggctttc tcttcccgaa agccgtcgcg gacttcaacg
    25141 cggcggtttc cattggcacg accaatatcc cgatcgcgat cggactaatc ctgatgatgt
    25201 acccgccgct ggcgaaggtg cgctatgacg aattgggcga tgttttccgt aactggaaaa
    25261 ttctcagcct ttcactcgtg cagaactggg tgatcggtcc cgttttgatg tttgtactgg
    25321 cgatcgtttt cttgcgtggt tatcccgagt atatgacggg tctcattctc attggccttg
    25381 cgcggtgtat tgccatggtc attgtgtgga acgaactggc aaaaggcgat aacgaatatg
    25441 cagcgggact ggttgcgttc aatagcatct ttcaggtttt gttctacagc ctgtacgcct
    25501 acgtattcat caccatcctg ccgacatggt tcggcatgac aggcaacatc gtgaacatta
    25561 ccatcggtga gattgccaga agtgtgttca tctatctggg tattccattc atcgcaggct
    25621 ttttcacccg cttcttcctt gtccgcgcga agggcaagga ttggtatcac aaggtcttta
    25681 ttcccaagat cagcccgttg accctggtgg cgttgctctt caccattgtg gtcatgtttt
    25741 cgcttaaggg tgacattatc gtgcagttgc cgttggacgt ggtcagaatc gccattcctt
    25801 tgctgatcta ctttgtgatc atgttcctgg tcagtttctt catgggtaaa tccatcggcg
    25861 cggattattc gcggacaacc acactttcgt tcactgctgc cagcaacaac tttgaactgg
    25921 caatcgcagt ggctgtggcg gtctttggaa tcaactccgg cgcagcgttt gcagcggtca
    25981 ttggcccatt gatcgaggtg cctgtgatga tcgccctggt aaatgtggcc ttcttctttc
    26041 aaaagagata ttttggcggc gtggtgagtt ctgccccagc cagatagacg atatccccga
    26101 cataaaaaaa cagtggtcaa ccttgaattg ggttgaccac tgtttttatg tgggctcatt
    26161 tgtaaagtga cgtttgagtt tcagcaatgg gtcttcgaga atgttccagg ttagtaatgc
    26221 caatacggtt gtgatggcaa ctgtcagcag gctgtaggcg gcgtagtatt cccatccgcg
    26281 tactttggct ttgaccatga tgtcgagcag ggtcagggcc acgggcatgt ggaagaggta
    26341 catcgagtag ctgtatttgc cgaagaagac cagcccgcca ttggcgaaaa attttcgaat
    26401 caacgaagtc tctgcgccgc tggtgaggaa aacgatcatg gcggcggagg aaaatgccag
    26461 cagggtgtat ccaatggtgc cgaggggaat attcctgtat aacggatgcg gccctgcggg
    26521 gctggcaatg aacgccagat acaggaacag gatcatcgtt atgatggaga cgggcaatgc
    26581 aaatcgtttg gctgtctcct gccagtttgt gggtgtgacg aagaagatgg cggttaatgc
    26641 gccaagggcc aggccctcaa aacgggtgaa ggtgttgtag aaaaagacaa caggcaattg
    26701 ctccgaatct ggtgagagca ggatggccgc cgtgcgaatc agcagggaga ggatgacaat
    26761 gcctgcgctg atccatgtca atgtgtgttt gttgacaatg cgtacgatgg cgggccagat
    26821 caaatagaac tgttcttcga ttgccagtga ccaggtaaca ctcaaatagg cggaaggata
    26881 gatcgcgaac aggtacagcc agttctgctg atagagcagg aaggtcggca aataagggat
    26941 gaacgaatcg gcggtctctg ccacagcggg gacgaatttc actgcgatga ggaaaagcgc
    27001 aagagccgtg tagtacaacg gaaaaatgcg cagcacccgc cgcgcataga agttcttgta
    27061 ataatttttt tcctcccttg tcttcagcag gatggacgtg atcagaaagc cagagagtac
    27121 gaagaaaata tccacgccca tccagccgac cagtgacagg cgtgtgatga aatgtaaaaa
    27181 cccattggcg gtgaagtaat cagcccgttt tagaacgtgg tgcgcaatga cgagcaggat
    27241 cgcgataccc cgcaatccat ccaattcctt tgagcggttg ttcatgatgc ggcgatcagc
    27301 tcatcatatt ttttgcgcac ttcgcgaatg tcgtcccaca tttgggcttt gggcgaacca
    27361 ggttccttcg agggattcct caacaagtag gaggggtgaa agatcggcat gatccagcgg
    27421 ccctgtgatt ctgtccactt gccgcgcagg gaggtgattc cagtcttgcg caggatggaa
    27481 tagctggcta cgttcccggt caacagcatc acaagcgggt cgatcagtcg gatgatctcg
    27541 aaaacataag gacggtaaaa atcggtttcg gcatcggtcg gtttgcggaa ggtcttgcct
    27601 tcctcaccgg gcggcatgcg gaaaacggag ttggtaatgt acacatcctt ttcggggtcg
    27661 aagtctgctg cttgcaggat cttgtcgagc atctggcctg cacgccctac aaaaggtttg
    27721 ccctgaatat tttcctcggg gccgggtgct tcaccgatga tcaataatct ggctttcggg
    27781 ttgccacggc tgataacgat attggtgcca gcctttgcca gcggatcgtc gttcatgcgt
    27841 ttcatcgcgc tgaataattc ttcgagcgat gaatattgtt tggcgctagg ggtgggttgg
    27901 gtcatggtct gctcctcgca aggggatgag ttaatttgat tctaccctat gaggcatctc
    27961 ataacgtgct cttttctttt acttcttttg gtgtttgtag tgaacatctg acaatgaacg
    28021 caatcgttct gtcgccactt cgggggtgat gtctcgttgc ggcatggcga gcatttcgta
    28081 gccgaccata aattttcgga ccgtggccga gcgcagtagc ggcggataaa aatgcgcatg
    28141 cagagtccac tcgggatgtg gctgcccatc agcaggagct tgatggaatc ccattgaata
    28201 ggggaatgaa atctcgaaca aattgtcgta tcttatcgtg acctgtttga agacttcagc
    28261 caatgcagag atttccaccg ctgacagttc cttcaacgaa gcggatgcgc gatgcgcgct
    28321 cacaagcacc tcgaacggcc acaccgccca aaaagggacc aacgctgtga aatggtcgtt
    28381 ggagaatagg atgcggtctt tgcgtttgtg ttcttctttc acataatcca ccagcaatgg
    28441 actgttgttc ttcttgaagt attccgtttg cgtggcaagt tctttgacaa tttcattcgg
    28501 cagttgggac tgtgaccaaa gctggctgtg cgggtgcgga ttggaacatc ccatcattgc
    28561 gcctttgttt tcaaagacct gcacatattt gatgtaatcc aactccgcga gtcttgcaga
    28621 ttcatttgtc caggtgttga ggacgttttc gatgacaggc aattcgagtt cgggcaacgt
    28681 caggtcgtgg cggggggaga agcaaactac cagacattga ccctgttcgg gtgcagagac
    28741 aagcaacggg tgggatgaag cggaagcgtc ttcttcaggg gcatctggca gcagagaagc
    28801 aaagtcgttg ttgaagacgt aggtgccagc ataatccgaa ttgaaaacgc cgccagcccg
    28861 ttcgttgcgc ggacagagat agcaggtggg gtcgtaggcg gggagggttt cgggggaaag
    28921 tttttcgacc tgcccaagcc agggacgttt ggcacggtgc ggcgagacga gcacccattc
    28981 gcccgtgagg gcgttatagc ggcggtgagg attgtttaac atggttattc cttctcaaat
    29041 atgatttcga tccattcaag gatttcgtgg tttcgggttt gttggagctt gatttcgatc
    29101 tggctgtttg aacaggcagt ttgcgttttc gttgcgcgcc cgctgaagta atgctcgatg
    29161 atggtcgtgc tgttgtgaag tgaagtatgg ctgattgtac aactgccgtc gccaaatggg
    29221 acgatgccga tgcggagtga ttctgaaata ccatcgtcgg ttagggatgc gacgactagc
    29281 gggacatcgg tgtgaaggat gtctttgtag tgtttcggcg caggttttcc atcgaggtga
    29341 tgggtgcgaa gtgcaaggag gacggtcttg tcgttttcga tgcgctccca cgagggagaa
    29401 aagccagcgc gcagaccacc ctgccatttt gcatggacgg gataggtttg aaaaataact
    29461 tgcttgcgag cgatggcggc aagcccattg aattttgcaa tgacttcgtc tgtgggcaag
    29521 ccgccacttt catctccgtc gaagcagtgc agggagccga gcgtgcctga ggcgatggtg
    29581 tcgaaccaaa tatcgggcgc ggattcccac tcgtagcctg aggaactgta agtgggcatt
    29641 cccaactcgc cgcacaaact gctgaaaaag acgcggcgat tggtttcaag gtcgtagtca
    29701 ccgatgcaat agacgaggtc atcggggctg tgcaaatcgt agtattcaat gagtagcggc
    29761 gagagaccgt agtacatgat gacgaggttt gggttttctt ctttcatcgc gccgaccaca
    29821 acttccaatc ccttttgcag aaggcgttcg cctgcccaat tcatgtcggt tggggcggcg
    29881 acatcgagcg agggaagttc gtagccaaag tcaaatttga tgaggtcagg tttgtagcgg
    29941 cggatgaatt ctttggcgcg ttcgctcaaa actttttgga cgatgggttg tgtgacatcg
    30001 aagatgccgt agcgggaggt ttgatggtcg
//
LOCUS       DAOQJC010000043        29882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_662_length_29882_cov_9.358165, whole genome shotgun sequence.
ACCESSION   DAOQJC010000043 DAOQJC010000000
VERSION     DAOQJC010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 29882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29882
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_662_length_29882_cov_9.358165"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="PLE39_08470"
     CDS             <1..1389
                     /locus_tag="PLE39_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68401.1"
                     /translation="KNRLLDGLESAAGGIPRAAMDKLISSLGKRVFVMHNVHEKMPVL
                     MQTRWAMNFLAGPMTRAQIRDLNLLADADSTRRASPMPAPAPKKESAPQPSVEAFQPV
                     SIPSASQPAAPQPATLQSAPARQSTPASGFTTKPSIPAGIREYYLPQNYSLPEAFQAA
                     GRAMPSQAMIQGIIYRPSLLASAQVRLLDRKHGVDSELDKVVLVDEMDRRGIVRWEDY
                     SYGGPSLDRIEAMPAATARFSTIDAPLNNTKMIAALQKDFTDWVFRNSAVTARANQAL
                     KVFAGPDVSQAEFMKTCADTARDARDAEIAKKTASIEKKIKALEDKLAREERELRQDE
                     EDLQNRNIETGINGIEVVAGLFGVGRKKSVSTPISKYRMAQNAKAEVQESVDAINQYR
                     KDLNALLRERDDMTAEINNRWGSVVNEISEVSIKPKKTDIYVNLFGVAWKPYYLVQSG
                     DETIELPAFGAE"
     gene            1485..2072
                     /locus_tag="PLE39_08475"
     CDS             1485..2072
                     /locus_tag="PLE39_08475"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNE68402.1"
                     /translation="MSIFILLLALAIWGIVHSFLASHFAKGMISLKTGGADFYRLTYN
                     VFALISFAPILYLMRTLPDQPIYQVASPWNLVMFGGQLFALLMLLIAFLQTDSLSFVG
                     LRQLFEKETTGALVTRGMYRIVRHPLYTFGLLFIWLTSTMTQNSLTVYCGATLYILIG
                     TYFEERKLLLDFGEAYAEYQRKTPMLIPGLVLGRK"
     gene            complement(2368..2898)
                     /locus_tag="PLE39_08480"
     CDS             complement(2368..2898)
                     /locus_tag="PLE39_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68403.1"
                     /translation="MSEVMQLLKCPSCGGPLEPPAGESTMKCLYCGSVVAIPESLRVQ
                     KQSTGYSQPSIFAGVDTSAMVGFGTQWGEVVQLAQSGNKAEAVKRYMALTGNDETSAT
                     YTVNHLAHTQMVTAQNIQQMYAPMMAQANETVKNVTRMSMWIGCGITALVTVIILMTL
                     IPVLIGVFASIMAIFR"
     gene            3010..3807
                     /locus_tag="PLE39_08485"
     CDS             3010..3807
                     /locus_tag="PLE39_08485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNE68404.1"
                     /translation="MKRFAALTLTIFLIACTRSVTGSYTPIPLYSTKIPITPTLNPVP
                     VATETATPSCVDALLQVKIMPLGDSITYGEGDPNRGGYRTKLAEFLLEDGIAFDFVGS
                     QRSGEAVLADPDNEGHPGWRITNIKDAIASEGWLETYQPDIILLHIGSNDLRNGRGIY
                     ARDHLSALLDDILVRLPHVHVIVAQIIPTRWGSEIDHQIYNNAIPNVAASKGGQVSVV
                     DMQEILVKDDFMTLYHPSTSGYEKMAQAWRAAIHALGLQPNCAISQK"
     gene            complement(3925..4830)
                     /locus_tag="PLE39_08490"
     CDS             complement(3925..4830)
                     /locus_tag="PLE39_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNE68405.1"
                     /translation="MKKFFKWLGIVLGSLLGVILIAFLGLALKGNSMLGKKYDVQVEK
                     ITIPTDAEAVARGEHWVMAECIGCHGHDLSGGEFFAAPFGYIDAKNLTPGNGGVGSDF
                     TDDDWVRALRHGVSSEGDSLLIMPAQFFRYYSDEDLGDIVAYLKTLPPVDKETREPSF
                     NLMGKALMGAGAFGESTVVAAYIIDNPLENVSYPPAGVTAEYGEYIVNVSGCRDCHGA
                     DLSGGKSADPSAINAPNLTPGGELVAWEEADFIKAIREGEAKSGHKLDPAQMPWEHYK
                     FFSDDELKALWAYLQSLPKLATTIP"
     gene            5060..6439
                     /locus_tag="PLE39_08495"
     CDS             5060..6439
                     /locus_tag="PLE39_08495"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HNE68406.1"
                     /translation="MTTLLIKNAYIVTMDNHQREIPEGGIFIRNGLIQEVGPVNELPA
                     TADEVLDLKGHVVFPGLVNTHHHFYQTLTRAVPAAQDANLFNWLKTLYPIWAKLQPDD
                     IFISTQTALAELALSGCTTASDHLYLFPNGSKLDDEIAAASEIGVRLQASRGSMSLGE
                     SKGGLPPDSVVDSEENILKDSQRLIEKYHDAKPGAMTQIVLAPCSPFSVTSDLMKQSA
                     KMAREYGVHLHTHLAETEDEEKFCMQMFGHRPVGYMQEVDWVGEDVWFAHAVWVNDEE
                     IKVFAKHNCGVAHCPTSNMRLASGIAPIKEYRKAGVNVGLGVDGSASNDGSHLLAEVR
                     NAMLVSRVKEGLTGYSLSNDPNRKLMTAREALHLGTRGGAAVLGRTDIGSLEAGKCAD
                     FFAVNLNRLGFAGMHDPVSAVVFGQPVNVDYTVVNGKFVVKEGRVVTIDQEKLIEKHN
                     KAAKRLLAG"
     gene            6457..6771
                     /locus_tag="PLE39_08500"
     CDS             6457..6771
                     /locus_tag="PLE39_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68407.1"
                     /translation="MFDSVLPIIFSWPFVVFSIIASVVGILYEMPLLVVVGALTIVPF
                     SYQLNNVQYFRGFALLLPVLQLFSAWALKEERNVWAWVLFAPTIAIILWLFVVNSIIG
                     FF"
     gene            6792..8582
                     /gene="ade"
                     /locus_tag="PLE39_08505"
     CDS             6792..8582
                     /gene="ade"
                     /locus_tag="PLE39_08505"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNE68408.1"
                     /translation="MTPSTKLTRSLVDVAMGRTPADLVIRGGKWVSVQSGEIIPHTDI
                     AIVNGHVAFVGHDASHAIGKKTKVIEANGRYLVPGLLDGHMHVESGMVTVTEFVRTVA
                     VRGTTGMFIDPHEIANVFGLKGVKLMVDEAQKQPIHVWVQMPSCVPSAPGLETPGSSI
                     GPKEVAEAMKWKGIIGLGEMMNFPGVFMGDKKMLDEMSATHAAGKTIGGHYASPDLGL
                     PFHGYVAGGAEDDHEGTRLEDAIARVRQGMKAMLRYGSAWHDVANQVKAITEKKMDSR
                     RFILCTDDSHAETLTVEGHMDRVLRHAIAEGLNPMAAIQMMTINTAEHFGLTKEMGMI
                     APGRWADVLLVEDLMNFKADVVIAKGQVIAEKGELLVKLPAVKYPRWATRSVHLKRPL
                     KAEDFVLRTRAAHGSDVDAHVIGVIENQAPTRHLRMKVKAENGEVKADMKRDLAKIAV
                     VERHRATGKVMVGLISGLGFTRPCAIASTVAHDSHHMIVVGTDDGNMAIAANELAQCG
                     GGQVVVMNGEVIGRVELQIAGLMSSERADVVAKKAGTVLDGFKTCGSELNNPNMQLSL
                     SALVVIPELRISDKGLVDVTNFEFISVLDE"
     gene            8590..9306
                     /locus_tag="PLE39_08510"
     CDS             8590..9306
                     /locus_tag="PLE39_08510"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNE68409.1"
                     /translation="MSNFPFQRLQVDLAALIARTPAGQRLPSEPELAKQMGVSRATLR
                     EAMRAFETQGLIRRRQGSGTFVVGKMRGLLDSGLEVLESLETMAKRLGLEVSVSDLSV
                     DSLLADADAATALGVAVDSPLTRVKRVVRADNRPVAYLVDILPEAVLRVEDLPADFHG
                     SVLDFLLDRGDPLKTSRANVSAIGGTADVAKALEIQRGDVLLHFYSQLFDDEGRIVDY
                     SLSYFIPGYFHFHIMRRVGG"
     gene            9374..10567
                     /locus_tag="PLE39_08515"
     CDS             9374..10567
                     /locus_tag="PLE39_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HNE68410.1"
                     /translation="MSIQEIQKRVQASREDIIEFMREIVAIPSMDSQLKDVGERIAKE
                     MNKLGFDEVRFDKMGNIMGRIGNGKRVIVYDSHVDTVGIGDPHEWEWDPFKGKIEDGI
                     LYARGACDEKGSTPGMIYGLAMARDLGLLEGWTAYYFGNMEEWCDGIAPNTFVEVDPK
                     VKPDFVVIGEPTKMLVYRGHKGRLEMKVTAKGKSAHAASNHLGDNAIYKLLPVIAGIR
                     DLEPKLGDHEFLGHGKITVSDLHVKTPSINAVPDEAVIFIDRRMTFGETKEMVKKQVE
                     DLIPAEFKDSVKLEELFYDEPSYTGFVFPVDKYFPAWALEAEHPLVQAGQEARKQIGL
                     PEAASGKWNFSTNGIYWAGKAGIPSIGFGPGDEETAHTVRDSVSLEDMVKATEFYAIL
                     PSLIK"
     gene            10613..11134
                     /locus_tag="PLE39_08520"
     CDS             10613..11134
                     /locus_tag="PLE39_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNE68411.1"
                     /translation="MKTLEIRRHSIRDHAGDHLNQNGITLARLIGQNSGPFDRVVTSA
                     LPRAFETAIAMGFAVDEQTELLNTYGHAVEKEAPWPQTFDFYAEVIKHNGAPARYAGK
                     LAKFHASIMDFISEGRSALVVTHGGVVELSIVGCLPGFDFSQWGDPVDYCEGARLYWD
                     DKFVDAEVLRVNR"
     gene            11162..12157
                     /locus_tag="PLE39_08525"
     CDS             11162..12157
                     /locus_tag="PLE39_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE68412.1"
                     /translation="MKRKSVFFTSLLLVMALAVSVLAGCSSAKPKVALMLARGGLGDK
                     AFNDSANEGLQKAMQELGVEVKTFDYQQDTQSENVRKAAQDGYQLVIGLGSENSAAIT
                     EIAKEFPDTKFALIDATAEGSNVTSVTFSELEGDFLAGALAALLSENNKVAYLGGADV
                     LVIRRIQFGFEQGVKYVKPDAEIVVQYVAGKDDFSGFSKPEDANAIAAQMYADGVDLI
                     YAAAGGSTLGAIDAAKAAGRLIVTTGSDQRYLAPEVVVTSRTKNMNQAVFMLIESFTK
                     KTLQSGTIQLDYKSGGIGIAPLSADLVPASVLSAFEAIQTDLESGNIKLEPFVSQ"
     gene            12194..12577
                     /locus_tag="PLE39_08530"
     CDS             12194..12577
                     /locus_tag="PLE39_08530"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNE68413.1"
                     /translation="MATIKNFEDIESWKKARELAGYVYTLTRKGGFERDFSLKNQIQD
                     AAGSVMHNIAEGFESGYDPEFVRFLKMARRSAGEVQSELYLALDTGYISQNELEKAYK
                     LAVDAKKLINGLISYLSKDQKTKDK"
     gene            12609..13598
                     /locus_tag="PLE39_08535"
     CDS             12609..13598
                     /locus_tag="PLE39_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNE68414.1"
                     /translation="MQTNFRGRDFIGDLDFTKEEVETVLEVAWDLKRKRALGEPHPYL
                     RDKVLAMLFFFSSTRTRGSFEAGMAQLGGHGAFIDSNTTQISHGDTPTEIGEIYGRYF
                     DGIAIRHCDYGDGNKYLNDVAKSSRAPVLNMQCDVYHPFQCLADLMTIMEKKGKDLRK
                     KKIVVSWAYAASYLKPISVPQSLILQMPRFGMDVTLAYPPEFPLMPDIVEQAKDEAKK
                     AGTAFEIIDSKDGMTEACKDADVIYAKSWGPLLTTTDKVEGKRLQDMYKNWLMDDAKL
                     KVAKEGAIYMHPLPADRDIEVTSNVLDGPQSVVFDEAENRLHAQKAVMALTMS"
     gene            13652..14602
                     /gene="arcC"
                     /locus_tag="PLE39_08540"
     CDS             13652..14602
                     /gene="arcC"
                     /locus_tag="PLE39_08540"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNE68415.1"
                     /translation="MTKFAVVAIGGNALIKDDKHVSVEDQEQALRETAIHLVDMIEAG
                     WDLAVGHGNGPQVGFILRRSEIAAKVEGMHEVPLDVCGADSQGAIGYELQQALRNEFF
                     KRKVNKKAATIVTQVRVDQADPAFQKPSKPIGSFMDEAEAKRREKEMGWSVVEDAGRG
                     WRRVVASPLPKEIVELDAVKTLLNAGQVVITVGGGGIPVVDRGNGELAGTAAVIDKDF
                     ASSLLAQSINADLFLIATAVEKVAINFGKPEQKWLDTMTVAEAKAYLEEGKHFAKGSM
                     APKIQAAIWYLENGGKEALITNPENIGRALKRETGTWIVK"
     gene            14642..16597
                     /locus_tag="PLE39_08545"
     CDS             14642..16597
                     /locus_tag="PLE39_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68416.1"
                     /translation="MKRIHPSLTLLAAAVLAYGLLIPQLGFYWDDLPMSWIRYQLGFE
                     AMTKYFSTNRPIWALLYQVTTRILPHLPIYWQVFALFWRWLGAVVLWNLAKELFPQRE
                     KFALTLGLLFLLYPGFNQQWVSYLYSHFFLVLFFFLFSQLLMLRGKTIPALVFSALNL
                     WMMEYFFVLELIRPFIIWTSLRDEVDPKERIKHTLNLWTPYLAVFALAVLSRIFIFNN
                     QIYQFSLKDELVKAPLATVLMLAQNVLSSFFVVTVAAWGQAFQFPNPLLDGPRTIAVY
                     AFVLLAVAALTLFGLWNQVSVKEGSGSTSVWRWALLLGAVMLVLAGPPFWLTNVPVSL
                     GFPANRATLSFMLGVSFVLAGLFDLLPQKFYGTVVVLFVMLAAGRQFLWSNEFRRDWA
                     SHKNMFWQMTWRAPGLEKDTIVLMNEELSFYADNSLGAALNLIYAPENHSDAIDYVLF
                     YPTNRESLSLSLDTPVNYDFLAGQFSGNTSQTVVFYYAPPKCLRLLDPEIDLENRLIP
                     DDTFLRDAALLSSTSPILSRQTSRMPAAYGNEPVHGWCYYFEKADLARQMGDWETVTQ
                     LGDVAFALDDYPNDPVERFVFIEGYAHTGNWERAVELSTASYKVSKAYVGPLLCRLWS
                     RIARETEDTSERKVTFDVVQSELECSP"
     gene            16703..17998
                     /locus_tag="PLE39_08550"
     CDS             16703..17998
                     /locus_tag="PLE39_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE68417.1"
                     /translation="MRKNVTRLSMFALILMLVLSACGGGQSAPAATEAPAGGDATEAP
                     AATEAPAATEAPAAEEAGGFQIPDVEEGKFNVAMVLIGPHDDGGWSQAHYEGLVYIEE
                     NVPNVHTAYIENVPEGADSEQVFRSLSRKGFNLIFGTSFGFGDPMAAVAEEFPDVMFI
                     HLTGINSNETNFGNLMGAMEDMKYLAGMLAGARAKADGNPKLGYMATFPIPEEIRLGN
                     AIALGMKKTCPECTMDVRWINTWHDPIIEKEAAASLFDAGAQVVFTGADTPAVADVAQ
                     EKGKWGVTYDWYGSCKVDACLTAPYWVWGPVYSQITEEVIAGTYKPGWQYFDADTGAL
                     GLYGFMEGQEMQPGVKDLDPEVIAMVQETLAQMLAGEFTRFDVFTGPIMDNQGNVVLP
                     EGQSLQQVDLDAFPEFGLPCSIDVCMKWWAEGITAELPQ"
     gene            18099..19688
                     /locus_tag="PLE39_08555"
     CDS             18099..19688
                     /locus_tag="PLE39_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68418.1"
                     /translation="MTQTTPSTPEIPLAVEMRGIVKRFPGVLASNHVDFELRAGEIHG
                     LLGENGAGKSTLMNVLAGLYRQEAGIIKVKGKPVDFYSPRDAINHGIGMVHQHFMLVP
                     SQTVTENVLLGLDDPRFFMRLPEYDAKVAAVGERFGLKVDPRAKIWQLSVGEQQRVEL
                     LKMLYRGANVLIMDEPTAVLAPQEIDGLFDILRSMIAQGKSVIFISHKLNEVSEIADH
                     VTVLRNGRVTASSVPSKGVSKQELARLMVGRDIVFFVDKKPVEPGEKVLDVKDVHAEN
                     DKGLPALRGLSLNVRKGEIVGVAGVAGNGQNELSQVITGLRKCKKGEVFLGGDKVSNR
                     DTLFGIQHGMSYVPEDRTHVGSAPNLSVTDNVIMKNFRKPPISKGGLLDMTAAGKFAN
                     ELKQAYDIIVPNVNTPVRLLSGGNLQRVILAREISEHPDFMVAVQPTRGLDVGAIEGV
                     HKLLLAQREAGAAVLLISEELDELLGLCDRVYVIYEGKIMGEVPIPIGKREEDLIETI
                     GLMMTGTPLDQIQKEGVGAND"
     gene            19681..20811
                     /locus_tag="PLE39_08560"
     CDS             19681..20811
                     /locus_tag="PLE39_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE68419.1"
                     /translation="MTETTSPQKKSMGFKIRVEPRLDEPPAWHPVLVSLGAIIVALIL
                     GGILISFAGGDPIKSYLHIADASFGDTGVLSDTIVKATPILLAALACSVAFRMKLWNI
                     GAEGQFIMGAFGASAVVLAPLLAEDTPRWLFLTVMALAGMAAGAIWGFIPGYLKAKFN
                     VNEIISTLMMNYIAVAWINFWIFGVWSEGGFQMSPKFPNNAWLPRLLEYAKTYPALRG
                     LTTHAGLLLGIVAAVVLWLIVYRSRWGYEIRLIGDNPHAAKYAGINITGNIIYVMMLS
                     GALAGLGGMSEVSGVVHRLQTSPLAAGYGFTGIIVAWLAKLNPIVIVLVSVLFGALIL
                     AGREIQPSGVPKMIQGIILVSLIASDFLLRYRIRIVRADAEE"
     gene            20819..21787
                     /locus_tag="PLE39_08565"
     CDS             20819..21787
                     /locus_tag="PLE39_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE68420.1"
                     /translation="MDPIIILQAGVATGTVLLFATIGEIFAERSGVLNLGVEGMMLIG
                     AMTAFSTTIATGNPWLGVLAAMLAAGLISQIHAFITITLQADQVVSGLALTFVGTGIS
                     LVLGEGLSKAGTVSLMPNFSIPLLSQIPFLGPILFTKQSVLVYIGYLLVPLTWYYINH
                     TRPGLNLRAVGEYPSAADSLGISVFRLRYFYVFIGGMMAGLAGATISLAVAPGWFSEL
                     TTAGQGWIAVGLVIFAQWDPVRAMFGSYAFGALRRLILDIQGPTILFGFNNPFYYNPY
                     WGFFLQMLPYAFTVIVLVIGSREAIRKRLGAPAALGNPYVRGERGK"
     gene            21960..22691
                     /locus_tag="PLE39_08570"
     CDS             21960..22691
                     /locus_tag="PLE39_08570"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNE68421.1"
                     /translation="MSIIQTKSVADQVEEILRERIRDATYLPGSRMPSESELSDEMGV
                     SRATVRTVLAKLAVNGLIIRKQGDGTYVNARAQEASAHTGNLWDLVRLIENNGSKPFI
                     QPISITRKPASEKEAQALAVEAGEPLLLFKRLFLADQIPVIYSNNLVPLSFFRVSTDE
                     IDGQLHFRDILRQYCHQEIGFVITDIRSTLVNDELRHLLRSELGPTVLELQMTFYSRN
                     SQPLALGLNYFDDTALRLGLVQAWN"
     gene            22743..23876
                     /locus_tag="PLE39_08575"
     CDS             22743..23876
                     /locus_tag="PLE39_08575"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE68422.1"
                     /translation="MRSRLNALFDALLPVLATLAALGVGAIMLIFLKVNPVTAYKALW
                     DGAFGSSNAFAETLVKATPLLLVALGICISFRGDVINIGGEGQMIIGAVLATWVGLTF
                     TGLPGWLAVTLALVAGFLGGAIWGGIPGVLKAYFSVNEILSTVMMNAIAVQLMNFLLR
                     GPMIDPSQAELASKIPQTARLLDAFRLPRLAPTRLHLGALIAVLLAVAVYILLWRTTL
                     GYRIRAVGQNPNASRYAGIKVQRYMVLALLLSGAFAGLAGAVQVYGVNYRMITDGSSS
                     GFTGSAGFNGIVAALFGQLHPLWSIPASILFGALLVGANKMQRVVQVPSALVIALNGL
                     VVVFVVSSEILRKRRQRRRLADDETPPVAGSAANQSTQAETGT"
     gene            23873..24805
                     /locus_tag="PLE39_08580"
     CDS             23873..24805
                     /locus_tag="PLE39_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE68423.1"
                     /translation="MISDLFTVTVLIGILASGIRLATPYLYAAIGETFSQKSGVLNLG
                     VEGQMLLGAFAAFYVSLTTGNLWLGLLTAMLVGALMGLAMAYVTVNLHAQQGISGIGF
                     YLFGLGMSDLLFQKLMGTVETVKGFPEIYIPGLSDIPGIGEIFFSQNILVYGAYLLVP
                     LAWFVLNKTTLGLKIRSVGENPDAADSLGVSVARVRYFTIILGGVLSGIAGASLSIAL
                     LNVFQQNMTSGLGFIAVALVYFGGWRPVGVLGGALLFSLINSLQLWIQVLGIPIPSDI
                     AVMMPYVLTILVLVATVSRVRSPSALSKPFEREN"
     gene            24918..25979
                     /locus_tag="PLE39_08585"
     CDS             24918..25979
                     /locus_tag="PLE39_08585"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HNE68424.1"
                     /translation="MKKFALSLVIAFVLLLSACGTPAATEAPVVEAPATDAPAEVPAA
                     TEPPAAPAEPFRVAVVMPSATNDLAFSQSMYDALVRIQEEMGGPEAFEFVFSEGMFVV
                     DDAAAAIRDYATQGYDLVIAHGSQYGSSLQEIAPDFPETSFAWGTTADTFGQPNIFAY
                     EAASQEGGYVNGVLAATLSQSAVIGVVGPIETGDAKLYVDGFKAGVAATNPAVTVNVN
                     YIGSFSDVALASEAATTHISADADVMTGTAQMVVGAIGKAEENNVLWFGTQSNQSSLA
                     PKIVVASQVYHWEVTLREIIALIKEGTLGGTSFKANLANGGEVIEFNADYALSDEAKA
                     LAETTIQGIIDGTIKITLP"
     gene            26059..27612
                     /locus_tag="PLE39_08590"
     CDS             26059..27612
                     /locus_tag="PLE39_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68425.1"
                     /translation="MTESTKLPSGKPRIETIEMRGITKRFPGVIANDKADFDIHAGEV
                     HALLGENGAGKSTLMKILYGMYRPDEGEILLNGKPVTIASPNDSINLGIGMIHQHFML
                     VPTLTVAENVALGLPSTRGALTDLDRVSKRIVELAGIYGLKIDPSAYVWQLSVGQQQR
                     VEIIKALYRGAALLILDEPTAVLTPQEVDELFVIMRQMVKDGHALIFISHKLHEVVEI
                     SNRITVLRDGRRIGTRPTSETTKQDLANWMVGREVGFTPDRGKVEIGEARLKLEKVEC
                     GSDRGTPGLRGVSIEVRSGEILGIAGVSGNGQRELAEAITGLRKVTAGRVFLEGKDIT
                     NFAPGDITELNLSYIPEERMRDGMIKEFTIAENMILREHHKMPYSRMGFLHLGDISKH
                     ADELIAKFHVKTPSRETHAKNLSGGNIQKVVLAREISRNPRAIIAAQPTRGLDIGATE
                     YVREQLLVERRKGVAVMLISEDLDEILALSDRIAVIYEGQVMDIVPRAEATPERLGLL
                     MAGVHPEVG"
     gene            27829..>28686
                     /locus_tag="PLE39_08595"
     CDS             27829..>28686
                     /locus_tag="PLE39_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNE68426.1"
                     /translation="MNLWQEYKRPASVTEAIQALTSASGPAMPIAGGTDLLLDLKQGR
                     HAPIHTLIDLTFVTEMTALELRGNELFIGAAVPVNRVALDPLTGTHAQALVEACNLIA
                     GPQVRNTATLGGNVAHALPAADGTIALTALNAQAEVAGLSGTRRMPFTSLFLGPGKSA
                     IDKTKEIIVGFHLPLSTPHSASCFKRIMRPQGVALPILNCSVWLERDAEMVKDVHIAV
                     GPGSSVPFRATDAENALRGKSLNEETFNQTLDTLLGQAQFRTSARRATADYRKHIVGG
                     LFKDVLDTAW"
     assembly_gap    28687..28786
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28787..>29882
                     /locus_tag="PLE39_08600"
     CDS             <28787..>29882
                     /locus_tag="PLE39_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNE68427.1"
                     /translation="RERLRLTGTKIGCEEAECGACTVLVDGQPMMSCVYPAERADGKV
                     VTTIEGLAQRVHEEMKLHPLQEAFVEHGAVQCGFCIPGQIMTAYALLNRNLDPNNDDI
                     RFALKDTLCRCAGYPSIENAIIAAAQALRTGEPVQKPTHIPDSVHEHKTVGHKHLRPE
                     AVEKVTGEAIYTDDLKFDGMLYAKAKRAMIPHGFLKKLDTSKAKALKGVIAVLTAEDV
                     PAEKNHGLVIYDWPVMVGVGERVRYVGDALAIVAAESPELAEQASALIEAEFDLQPVI
                     TNPVMAREEGVPQLHEKGNLLKHIKVRKGDMDAGFAEADIVIEHTFHTPATDHAFIEP
                     ECSIAVPLPDGRMEIYVGSQIPYQDRTQVAR"
BASE COUNT         6780 a         7321 c         7982 g         7699 t
ORIGIN      
        1 aagaaccgct tgctcgacgg cctcgaaagc gccgctggcg gcatcccgcg cgctgcgatg
       61 gataaactca tttcatcgct tggcaagcgt gtatttgtca tgcacaatgt ccatgaaaaa
      121 atgcccgtac tcatgcaaac ccgttgggcg atgaatttcc tcgcaggccc catgacccga
      181 gcacagattc gtgacctcaa tcttttggct gatgcagatt caacgcgtcg cgcctcgccg
      241 atgcctgctc ctgctccgaa aaaagagtca gcacctcaac cttctgtaga ggccttccag
      301 cctgtttcga tcccctcggc ctcacaaccc gctgctccac aacctgcaac cctgcaatct
      361 gctcctgcgc gccaatccac acccgcaagt ggatttacga ccaaaccctc catcccagct
      421 gggattcgtg aatactatct cccacaaaat tacagcctgc ccgaagcctt ccaggcggct
      481 gggcgtgcca tgccttcaca ggccatgatt cagggaatca tataccgtcc atctctgttg
      541 gcatccgccc aagtgcgcct gcttgaccgt aaacacggcg tggattccga actcgacaaa
      601 gtcgtactcg tggatgagat ggatcgtcgc ggcattgtcc gctgggaaga ttactcctat
      661 ggcggcccct cgcttgacag gatcgaagcc atgcccgctg ccactgcccg cttcagcacc
      721 attgatgcgc ctctcaataa cacaaaaatg atagccgccc tgcaaaaaga ttttaccgat
      781 tgggtcttcc gcaactccgc agtcactgcc cgcgccaatc aggcgctcaa agtatttgcc
      841 ggcccagatg tcagccaggc tgagttcatg aaaacctgcg ccgataccgc ccgtgatgcc
      901 cgggatgcag agatcgcaaa gaagaccgcc tccatcgaaa agaagatcaa agcccttgaa
      961 gacaaactcg cccgcgaaga gcgtgaactg cgtcaggacg aggaagacct gcaaaaccgc
     1021 aatatcgaaa caggcatcaa cggcatcgaa gttgtagctg gtctttttgg tgttggccgt
     1081 aagaagagcg tctccactcc gatctcaaaa tatcgcatgg cacaaaatgc caaagccgag
     1141 gtccaggaat cggtggatgc catcaatcaa tacagaaaag atctgaacgc cctgttgcgc
     1201 gaacgcgatg acatgaccgc tgagatcaac aaccgctggg ggagcgtggt caatgagatc
     1261 agtgaagtaa gcatcaaacc caagaagact gatatctatg tcaacctctt tggcgtggct
     1321 tggaagccgt actatctcgt tcagtctggc gatgagacca ttgaactccc tgcttttggt
     1381 gcagagtaat ttaatgcatt aagcagattt ttcggcctga catggttttg aatgtaaata
     1441 gtgacgacct caggtcgtca ctatgcatta aaggaaaaaa tacaatgagc attttcattc
     1501 ttcttctcgc gcttgccatt tggggcatcg tacattcttt tctggcctcc cattttgcca
     1561 agggcatgat ctccctaaaa acaggcggcg cggattttta ccgcctcacc tacaatgtct
     1621 ttgccctgat cagtttcgcc cccatcctgt acctgatgcg taccctgcct gaccagccca
     1681 tctatcaggt cgcctctccg tggaacctgg ttatgtttgg aggccagctg tttgccctgc
     1741 tgatgctgtt gatcgccttc ctgcaaacag attcattgtc ctttgttggc ttacggcaat
     1801 tgtttgaaaa agaaaccacg ggcgccctcg tcacccgtgg tatgtatcga atcgtgcggc
     1861 atccgcttta cacttttggt ttgttgttca tctggttgac ttcaaccatg acccagaatt
     1921 cattgaccgt ttattgcggt gcgactctct atattttgat cggcacttat tttgaagagc
     1981 gcaaacttct gctcgatttt ggcgaggcct atgcggagta ccagcgcaaa acaccgatgc
     2041 tgatccccgg gcttgtgctc ggcagaaaat agaaaaatcc cgtggatttt tgcctcaatg
     2101 gcttctgtac ccttttttga gtccttcgtt tagggctttg taattttcgg catagaactc
     2161 tgtgtaaccg cacgcactgc aaacattggc gcgaaaatgg ctttgtgcgt ttttggggag
     2221 ccatacgaaa ggtcgttttt ctggttcagg ttcaatgatg ctgacgtaag gcggatgtcc
     2281 ctcaccgcca tgcacattca tccctgaaaa aacttcgttg gaaccacact ttgggcacgg
     2341 attcatattc acctcgcaaa aagacaatta tctaaaaatg gccatgatgc tggcaaagac
     2401 accgatcaaa acggggatca aggtcataag aatgatgacc gtcaccaaag ccgtgatccc
     2461 gcatccgatc cacatggaca tgcgggtaac atttttgacg gtttcattgg cctgtgccat
     2521 cattggcgca tacatctgtt gaatgttctg cgccgtgacc atttgagtgt gcgccagatg
     2581 attcacagtg tatgtggctg aagtttcgtc gttgcctgtc aatgccatgt agcgtttgac
     2641 ggcttccgct ttattgccac tttgcgccag ttgaacgacc tctccccatt gtgtgccaaa
     2701 accaaccatg gcgcttgtat caacgcctgc aaagatgctc ggttgggaat agcccgtgct
     2761 ttgcttttgt acgcgcaggc tttcaggaat ggccacaaca ctgccgcagt acagacactt
     2821 catggtgctt tcgcctgctg gcggttccag cggcccacca cacgacgggc attttagtaa
     2881 ttgcatcact tcagacattg gtgtaaacct ccaaaaatag atcttgccga aaaacgatgc
     2941 ggcattatcc cctgattgtc tgtttttcga aaccagacaa aggtatgact gtttggtaaa
     3001 ctcacttcga tgaaacgctt cgccgccctt accctgacga ttttcctcat cgcctgcacc
     3061 cgttctgtaa cgggatcgta cacccccatc cccttgtatt cgacgaaaat accgatcact
     3121 cccaccctga atcctgttcc tgttgcgaca gagacagcca ccccgtcatg tgttgatgcg
     3181 ttgctgcagg tgaagatcat gccgcttggt gattccatca cctatggcga aggcgatccg
     3241 aatcgcggcg gatatcgtac aaaactggcc gagtttcttt tggaagacgg catcgcgttc
     3301 gattttgtcg gttcgcagcg cagcggggag gctgtactgg cagacccgga caatgagggg
     3361 catccgggct ggaggattac aaatatcaaa gatgcgattg catcagaagg ctggctggaa
     3421 acctaccagc cagatatcat tctgctccat attggctcca atgacctgcg caatggcagg
     3481 ggaatttatg cccgcgacca tctttcggct ttgcttgacg atatccttgt gcgcctgccg
     3541 catgtacatg tgatcgtggc gcagatcatc cccacgcgct ggggttctga gatcgaccac
     3601 caaatttata acaatgcgat tccaaatgtg gccgcttcaa aaggcgggca ggtctcggtt
     3661 gtggatatgc aggagatcct ggtcaaggat gatttcatga cgctttatca ccccagcaca
     3721 agtggatacg aaaaaatggc acaggcctgg agggccgcca tccatgcttt ggggttacaa
     3781 ccgaactgtg caatttcaca aaaataaatt ccatcggggt ggttgggtaa ttctatttaa
     3841 actacaaagg cactgagata cagagtcgat cattattctc tctgtgtctc agtgcctcgt
     3901 tagtttggct ttctggcctt ttctttatgg gattgttgta gccagcttgg gcagtgattg
     3961 tagatatgcc cacaacgctt tcaattcatc gtcggagaaa aacttatagt gctcccacgg
     4021 catttgcgca gggtcaagtt tgtgtccgct cttggcctca ccttcgcgaa tggccttgat
     4081 aaagtctgct tcttcccatg cgaccaattc accaccgggg gttaggttgg gggcattgat
     4141 cgcagaaggg tcagcgcttt tgccaccaga aagatccgca ccatggcagt cacggcagcc
     4201 actcacgttg acgatgtatt cgccgtattc cgcagtgact cctgcaggcg gatacgaaac
     4261 attttccaat ggattgtcaa taatataagc agcaacaacc gtgctttccc caaaggctcc
     4321 tgctcccatc agtgccttgc ccatcaggtt aaagctgggt tcgcgggttt ctttatcaac
     4381 gggcggcagg gttttcaaat aagcgacgat atcaccaagg tcttcgtcac tgtaatatct
     4441 gaaaaactgc gcaggcatga tcagcagact gtcaccctca gaactgacac catggcggag
     4501 tgcgcgcacc caatcatcat cagtgaagtc actgccgacc ccgccattgc cgggggtcag
     4561 attttttgca tcaatatacc caaagggtgc ggcaaagaat tcaccgccag atagatcgtg
     4621 accatgacag ccaatacatt ctgccatcac ccaatgctcg ccgcgggcaa cagcttctgc
     4681 atcggtgggg atggtgattt tctcgacctg tacatcgtac ttcttgccaa gcatggaatt
     4741 gcctttgaga gccagcccaa ggaatgcgat caatatcaca ccaagcaggc ttccaagcac
     4801 aataccgagc catttgaaaa acttcttcat attacattct ctcctcatta atctataaaa
     4861 ttttcattcc aaccgataat acgaccattt ttatctattt acaagccata catacacaca
     4921 actccattac attttggaat gaaatgacac tcacgaaaaa tattggcaca acccatttga
     4981 catcctcctc cttttttgct aaactagaag ttgtcagaca actacgccaa ttgcaacttg
     5041 tcaaaggcag ccttttccca tgacaactct actcataaaa aatgcctata tcgtcaccat
     5101 ggacaatcac caacgggaga ttcctgaagg tggcatcttc attcgcaatg gtctcattca
     5161 agaagttggt ccggtaaatg aactaccagc cacagctgat gaagttcttg atctcaaggg
     5221 acatgttgtt ttcccgggcc tcgtcaatac ccatcatcat ttttatcaaa cactcacacg
     5281 tgccgtcccc gccgcacagg atgcaaatct attcaactgg ttgaagaccc tttacccgat
     5341 ctgggcgaag cttcaaccgg atgacatttt tatttcgact cagaccgcac tggcagaact
     5401 cgccctgtca ggatgcacca ccgcatcgga tcatctctac ctgtttccaa acggttcaaa
     5461 actggacgat gaaattgccg cagcctccga gatcggggtc cgcctgcagg catcgcgcgg
     5521 ttccatgagc ctcggtgaat ccaaaggcgg gctgcccccg gactcggttg tggatagcga
     5581 agagaatatt ctcaaagatt cacaacgcct tattgagaaa tatcatgatg cgaaacccgg
     5641 tgcaatgacc cagatcgttc ttgcaccctg ttcccccttc agtgtcacct ctgacctgat
     5701 gaagcaatct gcaaaaatgg cgcgcgaata tggcgtgcat ttacacaccc atcttgctga
     5761 aacagaagat gaagaaaagt tttgtatgca aatgtttggt caccgtcctg ttggatacat
     5821 gcaggaagtg gactgggtgg gggaggatgt ctggtttgcg catgcagttt gggtgaatga
     5881 tgaggaaatt aaagtctttg ccaaacataa ttgcggtgtg gcacattgcc ccacttcaaa
     5941 tatgcgcctt gcctcaggta ttgcacccat taaggaatat cgcaaggcag gtgtaaacgt
     6001 tggcctcggt gtggatggct ctgcctccaa tgatggttcg catctgctgg cggaagtccg
     6061 caatgccatg cttgtctccc gtgtcaaaga aggattgacc ggatactccc tttccaacga
     6121 tcccaaccga aaattaatga ccgcccgtga agccctgcac cttggcacac gtggtggcgc
     6181 agctgttttg gggcgaaccg acattggcag cctcgaagct ggcaagtgtg ccgacttctt
     6241 tgctgttaac ttgaacaggc tcggctttgc tggcatgcac gaccccgtct ctgctgtggt
     6301 ttttgggcag cccgtcaacg tggattacac agttgtgaat ggaaagtttg ttgtaaagga
     6361 aggcagggtg gtaacgatcg accaggagaa attgatcgag aaacataaca aggccgccaa
     6421 acgacttttg gctggttgag aggagtctga aacaccatgt tcgatagtgt attgccaatt
     6481 attttcagct ggccttttgt tgtcttttct atcatcgcct cagtggtggg gatcctttat
     6541 gaaatgccgc tgttggttgt tgtgggggcg ttgacgattg tgccgttttc ttatcaattg
     6601 aataacgtgc aatattttcg cggttttgcc ttgcttttgc cggtgttgca gctcttttct
     6661 gcatgggcgc taaaagagga aagaaatgtt tgggcatggg tgttgtttgc tcccaccatt
     6721 gccatcattc tctggttgtt cgtggtcaat tccataatcg gatttttcta gtttgttctg
     6781 ggagattttt tatgacaccg tccacaaaac tcacccgctc cctcgttgat gttgccatgg
     6841 gacgtacccc tgccgacctt gtgatccgtg gtggaaagtg ggtcagcgtg cagtcgggtg
     6901 agatcattcc ccatactgat attgccattg tgaatggaca tgtagccttt gttgggcatg
     6961 atgccagcca tgccattggt aaaaagacaa aggtgatcga agcgaacggg cgataccttg
     7021 tacctggcct gctcgacgga cacatgcacg ttgaatcagg catggtcaca gtgaccgaat
     7081 ttgtgcggac tgtggcagta cgcggcacga ccggtatgtt tattgacccg catgagatcg
     7141 caaatgtatt cggcctcaaa ggtgtgaagt tgatggtgga tgaggcgcaa aagcagccca
     7201 tccatgtttg ggtgcaaatg ccgtcctgtg tgccttctgc gccagggctc gagactccgg
     7261 ggtcatccat tgggccgaag gaagtggccg aagccatgaa atggaaaggc atcatcggtc
     7321 ttggtgagat gatgaatttc cccggtgtat tcatgggcga taaaaagatg ctggacgaaa
     7381 tgtccgccac tcatgcggca gggaagacca ttggcgggca ttatgcttcg cctgatcttg
     7441 ggctgccatt tcatggatat gtggctggcg gcgccgaaga tgatcacgaa ggcacacgtc
     7501 tggaagatgc gattgcacgg gtgcggcagg gcatgaaagc catgctgcgt tacggttcgg
     7561 cctggcacga tgtagcgaat caagtgaagg cgatcactga aaagaaaatg gattcgcgcc
     7621 gtttcattct ttgcacagat gattcgcacg cggaaacgct tacagtggaa gggcacatgg
     7681 accgtgtgct gcgccatgcc attgctgaag gcttgaaccc gatggcggcg atccagatga
     7741 tgaccataaa taccgccgaa catttcggct taacgaaaga aatgggcatg atcgccccgg
     7801 ggcgctgggc tgatgtgctt ttggttgaag acctgatgaa ctttaaagcg gatgttgtaa
     7861 ttgccaaagg acaggtgatc gctgaaaagg gtgaattgct ggtcaaactt cctgcggtga
     7921 agtacccgcg ttgggcaacg agatcggtgc atttgaagcg acctctcaag gcggaggatt
     7981 ttgtccttcg gacgagagca gctcacgggt ccgatgtgga tgcgcatgtc attggcgtga
     8041 tcgaaaatca ggcgccgact cgtcatctgc ggatgaaggt caaagctgaa aatggggaag
     8101 tcaaagccga tatgaaacgt gacctggcga agatcgctgt ggtggaacgt catcgcgcga
     8161 ctggcaaggt gatggtgggg ttgatcagcg ggcttgggtt tacgcgcccc tgcgcaattg
     8221 cttcgactgt ggcgcatgac agtcaccata tgatcgtggt gggtacggat gatgggaaca
     8281 tggcgattgc cgccaatgaa ctggctcaat gtggaggcgg tcaggtggtg gtgatgaatg
     8341 gtgaagtgat cgggcgcgtg gaattgcaga tcgctggctt gatgtccagt gagcgtgcgg
     8401 atgtggtggc gaagaaggcg ggaactgtgc ttgatggatt caaaacttgc ggtagtgagt
     8461 tgaataaccc gaatatgcag ttgagtttgt ctgcccttgt ggtgattcct gaattgcgga
     8521 tttcagacaa agggcttgtg gatgtgacga attttgagtt tatctctgtt ttggatgagt
     8581 gaggcagata tgtcaaattt tccttttcaa cgattgcagg tggatctggc tgcattgatc
     8641 gcgcgaaccc ctgcggggca gcgtctgcca tcagagcctg aactggcgaa acaaatgggt
     8701 gtttcccgtg ccacgctccg tgaggccatg cgtgcatttg aaacccaggg tttgatccgt
     8761 cgtcgccagg gttcgggcac gtttgtggtg gggaagatgc ggggcttgct tgatagcggg
     8821 ctggaagttc ttgaaagcct cgaaaccatg gcaaagcgtt tgggtttgga agtttcagtg
     8881 agcgacctca gtgtagattc gcttctggca gatgccgatg cagccactgc cctgggtgtc
     8941 gccgtggatt cccccctgac acgcgtcaaa cgtgtggtgc gtgccgataa cagaccagtt
     9001 gcctatcttg tggatattct cccggaagca gtcctgcgtg ttgaagatct gcctgctgat
     9061 tttcacggtt ctgttctgga ctttttgctt gatcgcggcg atcctttgaa gacttcccgc
     9121 gcgaatgtca gtgccattgg tggaacggca gatgttgcca aggcgcttga aatccagcgc
     9181 ggtgacgtgt tgctccattt ttattcgcaa ttgtttgatg atgaaggcag gatcgtggat
     9241 tattcgttga gttatttcat ccccggctat ttccatttcc atattatgag gcgtgtcggc
     9301 ggataaggtg ctgtatttta caacgctggg gtgggcccga cgttgaatca tttttatgca
     9361 acaaggagtt caaatgtcaa ttcaggaaat acaaaaaaga gttcaggctt cccgtgagga
     9421 tatcatcgag tttatgcgcg agatcgttgc cattccctcg atggattccc agttgaagga
     9481 tgtgggtgaa cgcattgcca aagagatgaa taaacttggt tttgatgagg tccgctttga
     9541 caagatgggc aacatcatgg ggcgcattgg taacggaaaa cgggtcattg tttacgattc
     9601 acatgttgat actgtcggta ttggcgaccc gcacgaatgg gaatgggatc ctttcaaagg
     9661 caagatcgag gacggcattt tatacgcccg cggcgcttgt gacgaaaaag gctcaacccc
     9721 tggcatgatc tatggtctgg ccatggcccg tgatctgggt ctgctcgaag gctggacagc
     9781 ctattacttc ggtaacatgg aagagtggtg cgatggcatc gctcccaata cctttgttga
     9841 agtggaccca aaggtgaaac ccgattttgt ggtcatcggc gagccgacca agatgctggt
     9901 ttatcgcggg cataagggcc gccttgaaat gaaagtgacc gccaagggca aatcggctca
     9961 tgcggcgagc aatcatctgg gcgataatgc catctacaaa ctcctccctg tcattgcagg
    10021 gattcgtgac ctcgaaccca aacttggcga ccatgaattt ttggggcatg gcaagatcac
    10081 cgtttcagac ctgcatgtaa agaccccctc catcaatgct gtacctgatg aagccgttat
    10141 tttcatcgac cgccgtatga cctttggtga aaccaaggaa atggtcaaaa agcaggtgga
    10201 agatttgatc cctgcagaat tcaaagattc tgtgaagctc gaggaattgt tctacgatga
    10261 gccttcctac actgggtttg tcttccctgt ggataaatac ttcccagcct gggctttgga
    10321 agcggaacat ccgctggtgc aggctggtca ggaagcccgc aagcagattg gcctgcccga
    10381 ggcagccagt ggcaagtgga atttctccac gaatggcatt tactgggctg gcaaagctgg
    10441 cattccgtcc atcggttttg gccctggcga tgaagagacc gcacacactg tccgcgattc
    10501 tgtttcgctg gaagatatgg tcaaggccac cgagttttat gcgattttgc cgagcctgat
    10561 taaataatag atttgcaggg acacagtgat gctgtgtccc tgcatggtga atatgaaaac
    10621 cctcgaaatc cgccgacact ccatccgcga tcatgcgggt gaccatttga atcaaaacgg
    10681 gataacgctg gcccgtttga tcggtcagaa tagcggccca tttgatcgtg tggtcacttc
    10741 tgcgttgccg cgtgcctttg aaacagccat tgccatgggg tttgctgtgg atgagcaaac
    10801 tgaactgttg aacacctacg gccatgccgt ggaaaaagaa gcgccctggc cgcagacctt
    10861 cgacttttac gccgaagtga tcaagcataa cggtgcacct gcgcggtatg ccggtaaact
    10921 ggccaagttc catgccagca ttatggattt tatctccgag ggccgttcgg cattggtggt
    10981 aacccatgga ggtgtggttg agttgagcat tgttggctgc ctgccagggt ttgatttttc
    11041 acaatggggc gacccggtgg actattgtga aggcgcacgg ctttattggg atgataagtt
    11101 tgttgatgca gaagtattgc gcgtcaaccg ataaaaatca agcgaaggaa ctttataaac
    11161 aatgaaaaga aagtcagttt ttttcacatc tctcctgctc gttatggcgc tggctgtaag
    11221 tgtattggct gggtgcagca gtgccaagcc caaggtcgcg ctcatgcttg cgcgtggcgg
    11281 acttggcgac aaagcattca acgatagtgc caatgaaggc ctgcaaaaag ccatgcagga
    11341 actcggcgtg gaggtgaaaa ctttcgatta ccagcaggat acgcaaagtg aaaacgtccg
    11401 caaagccgca caggatggct atcaattggt cattgggctt ggctctgaaa actctgcagc
    11461 cattaccgag attgcgaagg aattccctga taccaaattc gcattgatcg atgcaacagc
    11521 tgaaggctcc aatgttactt cggtcacttt cagtgaactc gaaggtgatt ttcttgcggg
    11581 cgcattggcg gccttgttga gcgaaaataa caaagtggcc tacctcggcg gcgccgatgt
    11641 gctggtgatt cgccgcattc aattcggttt tgagcagggt gtcaaatacg tcaagccaga
    11701 tgccgagatc gttgttcaat atgttgctgg caaggatgat ttcagcggtt tctcaaaacc
    11761 tgaggatgcc aatgcaattg ccgcacaaat gtatgccgat ggcgtcgatc tcatttacgc
    11821 agcggcagga ggatccactc tcggggcgat cgatgccgct aaagcagcgg gcaggctcat
    11881 cgtcacaaca ggaagcgacc agcgttatct cgctcctgag gttgtggtca ccagccgcac
    11941 caagaacatg aatcaggccg tattcatgtt gatcgaatcc ttcacaaaaa agaccttgca
    12001 aagtgggacg atccaactcg actacaaatc tggtggtatt ggcatcgcgc ctctttctgc
    12061 tgaccttgta cctgcttcag ttttaagcgc attcgaagcc attcaaactg atcttgagtc
    12121 tggaaacatt aagttggaac cttttgttag tcaatagttg gctggtcgct ggtcctctgg
    12181 tcaaagagaa gacatggcaa caataaaaaa ttttgaagat attgaatctt ggaaaaaggc
    12241 ccgcgaattg gcaggctacg tttataccct gacccgaaaa ggaggttttg aacgtgactt
    12301 ttccttgaaa aaccaaatac aggatgctgc tggttctgtt atgcataata ttgccgaagg
    12361 ctttgaatca gggtatgacc cagagtttgt ccgttttttg aagatggcac gtcgttcagc
    12421 tggagaagtt caatcagaac tgtatcttgc tcttgataca ggttacattt cacagaatga
    12481 attggagaag gcctataagc ttgctgttga tgccaaaaaa ctcattaatg gtctgatctc
    12541 ttatttgagc aaagaccaaa aaacaaaaga caagtagaca aaaaaacaaa agactaggag
    12601 actaaatcat gcaaaccaat tttagaggcc gcgacttcat tggcgacctg gatttcacga
    12661 aagaggaagt tgaaaccgtt ttggaggtgg cctgggatct gaagcgcaag cgcgcactcg
    12721 gtgaaccgca cccctacctg cgcgataaag tgctggcgat gttgttcttc ttctcttcca
    12781 cccgaacccg tggatcgttt gaagcaggca tggcccagtt gggcggccat ggcgctttca
    12841 ttgacagcaa taccacccag atctcacatg gcgatacccc caccgagatc ggcgaaattt
    12901 atggccgcta ttttgacggc attgccattc gtcattgcga ttatggcgat ggaaacaaat
    12961 acttgaacga tgtcgccaaa tcaagccgcg caccggtgct gaacatgcaa tgtgatgtgt
    13021 accatccctt ccagtgcctt gccgatttga tgaccatcat ggaaaagaaa ggcaaagacc
    13081 ttcgcaagaa gaagatcgtc gtttcctggg cgtatgccgc ttcctacctc aagccgatct
    13141 ctgtgccgca atctttgatt ttgcaaatgc cgcgctttgg catggatgtg accctggctt
    13201 acccgcccga gtttccgctc atgcccgata tcgttgaaca ggcgaaggac gaagcaaaga
    13261 aggctggaac tgctttcgaa atcatcgata gcaaagacgg catgaccgaa gcctgcaaag
    13321 atgccgacgt gatctacgcc aaatcatggg gtccactgct caccactacc gacaaagtgg
    13381 aaggaaaacg cctgcaggat atgtacaaga attggttgat ggatgatgcc aaactcaagg
    13441 tcgccaagga aggcgcgatc tacatgcacc cattgcccgc cgaccgcgat atcgaagtga
    13501 cctccaacgt cctggatggt ccgcaatctg ttgtgttcga tgaagctgaa aaccgcctcc
    13561 acgcccaaaa ggctgtgatg gctctcacca tgtcctaaaa caattacagg gatacaacgc
    13621 cgttgtatcc ctgtaaacag gaaatttacc aatgaccaaa ttcgcagtcg tcgccattgg
    13681 cggcaatgcc ctgattaaag atgataaaca tgtttcagtg gaagaccagg aacaagccct
    13741 gcgcgaaacc gccatccacc ttgtggatat gatcgaagca ggctgggacc tggccgttgg
    13801 ccatggcaat ggtccacagg tcggttttat tttgcgtcgt tccgagatcg ctgcaaaagt
    13861 ggaaggcatg cacgaagttc cgctcgatgt ttgcggcgcc gattcgcaag gtgccatcgg
    13921 gtatgagctc caacaggctt tgcgtaatga atttttcaaa cgcaaggtca acaaaaaagc
    13981 tgccaccatc gtcacccagg tgcgcgtcga tcaagctgac cccgcttttc agaaacccag
    14041 caaacccatc ggctctttta tggacgaggc ggaagccaaa cgccgtgaaa aggaaatggg
    14101 ctggtcggtt gtggaagatg caggccgtgg ctggcggcgt gttgtagcct ctccgctgcc
    14161 gaaagagatc gttgaacttg atgcggtcaa gaccctgctg aacgcgggac aggttgtcat
    14221 cactgtgggc ggcggcggca tccctgtagt agatcgtggc aacggtgagc tcgcaggcac
    14281 cgctgcagtc attgacaagg actttgcttc atccctgctg gcgcaatcca ttaatgccga
    14341 tctgttcttg atcgccactg cggtcgaaaa ggttgccatt aattttggaa aacccgagca
    14401 aaaatggctc gataccatga ccgtcgctga agccaaagcc tatcttgaag agggcaagca
    14461 ctttgccaaa ggttccatgg ctccgaagat tcaggcagcc atctggtatc ttgaaaacgg
    14521 cggcaaggaa gcccttatca ccaatcctga aaatattggc cgcgccctca aacgcgaaac
    14581 tggaacatgg atcgttaaat aaaatgcagg ggcatagcgc cgctgtgccc ctgcagccaa
    14641 catgaaacgc attcatcctt cacttaccct cctggccgcc gcagtccttg cgtatggctt
    14701 gctcatccca caactcggct tttactggga tgatcttccc atgtcctgga ttcgctatca
    14761 acttgggttt gaagcgatga caaaatattt ttcaaccaac cgtccgatct gggcgttgtt
    14821 gtatcaagtt accacccgca tattgccgca tctgccaatc tattggcagg tctttgcgct
    14881 gttctggcgc tggttgggtg cagttgttct gtggaatctc gccaaagaat tatttcccca
    14941 gcgtgagaaa tttgccctca cgctgggcct gctttttttg ttgtatcctg gtttcaacca
    15001 acagtgggtc agttatcttt attctcattt cttccttgtt ctttttttct ttcttttttc
    15061 ccaactcctc atgctgcggg gcaaaacgat ccctgcattg gtcttttccg cgcttaatct
    15121 ttggatgatg gaatatttct tcgttcttga attgatccgc ccctttatca tctggacttc
    15181 ccttcgagat gaagtggacc cgaaagaacg gatcaaacat accctcaacc tgtggacgcc
    15241 gtatctggca gtgttcgccc ttgcggtttt atcgcgtatt ttcatcttta acaatcagat
    15301 ctaccaattc agtctgaagg acgagttggt gaaagcaccg cttgcgacgg tgttgatgct
    15361 cgcgcaaaat gtcctctcca gcttttttgt ggtgacggtg gcggcctggg ggcaggcttt
    15421 ccaatttcca aatcccctct tggatggtcc gcggacgatc gcggtctatg cttttgtttt
    15481 gctggctgtt gcggcgctga cgttgtttgg tctatggaat caggtttcgg ttaaagaggg
    15541 aagcggttca acttccgtgt ggcggtgggc acttttattg ggtgcggtca tgcttgtttt
    15601 ggcgggcccg ccgttttggt tgaccaatgt ccctgtttcg cttggttttc ccgccaatcg
    15661 agccactttg tctttcatgc tgggtgtgag ttttgttttg gcaggtttgt tcgatttgtt
    15721 gccgcaaaag ttttacggga cggttgttgt gctttttgtg atgttggcag caggcaggca
    15781 gtttttatgg tcaaacgagt tccgccgcga ttgggcatct cacaagaaca tgttttggca
    15841 gatgacatgg cgtgcgcctg gccttgagaa agacacgatc gttctaatga atgaagagtt
    15901 gtctttttat gccgataatt cactgggtgc cgccctcaac ctgatctatg caccggagaa
    15961 tcattctgat gcgattgatt atgtgttgtt ctatccgaca aaccgtgaaa gtttgtcttt
    16021 gagtttggat acgccggtga attacgattt tttggctggt cagttttcgg gtaatacctc
    16081 tcaaacggtt gtcttttact atgccccccc caaatgtctg cgcctgcttg accctgagat
    16141 cgatcttgaa aaccgcttga tccctgacga tacatttttg cgtgatgccg ccttgctttc
    16201 ttcaacttct ccaattctga gcaggcagac ttcgcgcatg cccgctgcct acggaaacga
    16261 gcctgttcat ggctggtgct actattttga aaaagccgac ctggcccgtc agatgggtga
    16321 ctgggaaact gttacccaat tgggcgatgt ggcctttgcc ctggacgatt atcctaatga
    16381 cccggttgag cggtttgttt ttatcgaagg ctacgctcac acagggaatt gggaaagggc
    16441 ggtagaatta tccacagctt cttacaaggt ttcaaaagca tatgtgggtc cattgctgtg
    16501 tagattatgg agccgtattg cccgcgaaac ggaggacaca tcagagcgga aagtcacctt
    16561 tgatgtagta caaagtgaac ttgagtgttc accgtaaaag tttataattt ggttttatat
    16621 gtgatcgggc tgaaaggagg ccaaaggaaa aaattggtct taagtctggt gcatagaaca
    16681 aatactaaac aaggagaaga aaatgcgtaa aaatgtaacc cgtctgtcca tgttcgcctt
    16741 gatcctcatg cttgtgcttt ctgcatgcgg tggtggtcag tccgccccgg ctgctactga
    16801 agcacctgct ggtggcgatg ccactgaggc tcccgccgca actgaggcgc ctgccgcaac
    16861 tgaagccccc gccgctgaag aagctggcgg cttccagatc cctgatgtgg aagaaggcaa
    16921 gttcaatgta gcgatggttt tgattggccc gcacgatgat ggtggttggt cgcaggctca
    16981 ttacgaaggc ctcgtttaca tcgaagagaa cgttcccaat gttcacactg cttacattga
    17041 aaacgttcct gagggtgcag attcagagca ggtgttccgc agcctttccc gcaagggctt
    17101 caacctgatc tttggtacct cgtttggctt tggtgacccg atggccgctg ttgccgagga
    17161 attccccgat gtgatgttca ttcacctcac cggtatcaat tccaatgaaa ccaactttgg
    17221 caacctgatg ggcgccatgg aagatatgaa gtacctggct ggtatgctgg ccggtgcccg
    17281 cgccaaggct gatggcaatc ccaagctcgg ctacatggcc accttcccga tccctgaaga
    17341 aattcgcctt ggtaacgcca ttgcattggg tatgaaaaag acctgtccgg aatgcacaat
    17401 ggatgtccgc tggatcaata cctggcacga cccgatcatt gaaaaggaag ctgctgcttc
    17461 tctgttcgat gccggtgccc aggttgtatt caccggtgct gatacccccg ccgttgctga
    17521 tgttgcccag gaaaagggca agtggggcgt aacctatgac tggtacggtt cctgcaaagt
    17581 ggatgcctgt ctcaccgccc cgtactgggt gtggggacct gtgtactccc agattaccga
    17641 agaagtgatc gctggcacct acaaaccggg ttggcagtat tttgatgctg acactggcgc
    17701 cctcggcctg tatggcttca tggaaggcca ggaaatgcag cccggcgtga aggaccttga
    17761 tcctgaagtg atcgccatgg tgcaggaaac ccttgcccaa atgctcgctg gcgaatttac
    17821 ccgcttcgat gtgttcaccg gtccgatcat ggacaatcag ggtaacgtgg ttctgcccga
    17881 aggccagagc ctgcaacagg ttgaccttga tgcgttccct gagttcggtc tgccctgttc
    17941 cattgatgtt tgtatgaagt ggtgggctga aggtattaca gcggaacttc cgcagtagtt
    18001 ttgtcgattt caaatgggct ggaattctgt aaagggttcc agcccttcat taagagtttt
    18061 attttcagtc gtgtttgttt tatcgcagga gcaacccagt gacacagaca acaccttcca
    18121 cacctgaaat accccttgcc gtggaaatgc gcggcattgt caagagattc cccggtgtat
    18181 tagccagtaa ccatgttgat tttgagttac gtgcaggcga gatccatggt ttgcttggcg
    18241 aaaatggcgc agggaaaagt actctgatga acgtcctggc tgggctatac cggcaggaag
    18301 caggaattat taaagtaaag ggcaagcctg tggattttta ttccccacgg gatgccatta
    18361 atcatgggat cggcatggtg catcaacatt ttatgcttgt cccttctcaa actgtcactg
    18421 agaatgtgct tctggggttg gatgatcccc gcttttttat gcgcttgcct gagtatgacg
    18481 caaaagttgc ggcagtcggt gagcgttttg gtttaaaggt ggatccacgt gcaaaaattt
    18541 ggcagctttc agttggtgag cagcaacgtg tggaactgct caaaatgctg tatcgcggcg
    18601 ccaatgtgtt gatcatggat gagcccacag cggtacttgc gccgcaggag attgacggtc
    18661 tttttgatat tttgcgctca atgattgctc agggtaaatc tgtcatattc atcagccata
    18721 aactcaacga ggtcagcgaa attgccgatc atgtcactgt tttgcggaat ggcagggtaa
    18781 ctgcttccag tgtgccttcg aagggtgtga gcaaacagga attggcgcgt ttgatggttg
    18841 ggcgtgatat cgtcttcttt gtggataaaa aacctgtgga gcccggcgaa aaggttctgg
    18901 atgtgaagga tgtccacgcg gaaaacgata aaggattgcc agccctgcgc ggactttcgc
    18961 tcaatgttcg caagggtgag atcgttggtg tggctggcgt tgcaggtaat ggtcaaaatg
    19021 aactttcgca ggtcattacc gggcttcgta aatgcaaaaa aggggaagtg tttttgggtg
    19081 gggataaggt cagtaatcgt gataccttat ttggaattca acacggcatg tcgtatgtcc
    19141 ctgaagatcg cactcatgtg gggtccgccc cgaatctcag cgtgaccgat aatgtcatca
    19201 tgaagaattt tcgcaagccg cccatttcaa aaggtggatt gctggatatg accgccgcag
    19261 ggaaatttgc caatgagttg aagcaggctt atgacattat tgtcccgaac gtaaatacgc
    19321 ccgtgcgcct tttatcgggt ggaaatttac agcgtgtcat ccttgcgcgt gaaatttcag
    19381 agcacccgga tttcatggtt gccgttcagc cgacgcgtgg tttggatgtt ggcgccatcg
    19441 aaggtgtgca taaattgttg ctggctcaac gtgaggcggg ggcggcagtg ttgttgattt
    19501 ctgaagagtt ggacgaatta ctcggcttat gtgaccgtgt gtatgttatt tatgaaggga
    19561 agatcatggg tgaagtgccc attcccatcg gcaagcgtga agaggatctg atcgaaacca
    19621 tcggtctgat gatgaccggc accccgttgg accaaatcca aaaagaagga gtcggcgcaa
    19681 atgactgaga caacttcacc acaaaagaaa tccatgggct tcaaaattcg agttgaaccg
    19741 cgactggatg agccacctgc ctggcaccct gtgcttgtgt ccttgggcgc gatcattgtg
    19801 gcgcttattt tgggcggtat tctgatttct tttgcaggcg gcgaccccat taaatcttat
    19861 ttgcatatcg cggatgcgtc ttttggcgac accggggttt tatcagacac cattgtgaag
    19921 gctaccccaa ttttgctggc cgccctggct tgttcggtgg ctttccgcat gaagctgtgg
    19981 aatattggtg ccgagggtca gttcatcatg ggagcatttg gggcaagcgc agttgtcctt
    20041 gctcctttgt tggcggagga taccccacgt tggttgtttc tgactgtgat ggcgctggca
    20101 ggcatggctg caggtgcgat ttgggggttt atccccggat acctcaaggc caaatttaac
    20161 gtcaatgaga ttatcagcac cttgatgatg aattacatcg ctgtggcttg gatcaatttt
    20221 tggatctttg gggtatggtc tgaaggtggt tttcagatgt caccgaagtt cccaaataat
    20281 gcatggcttc cgcgtctgct ggagtatgcg aagacctacc ccgccctgcg tggactaacg
    20341 actcatgcgg gccttttgtt ggggatcgtc gcggcggttg ttctctggct gatcgtgtat
    20401 cgcagtcgct gggggtatga aattcgtctc attggtgata atccacatgc ggcaaaatat
    20461 gcgggtatca atatcacagg gaacataatt tacgtgatga tgctttccgg ggcgttggct
    20521 gggttgggcg gtatgagtga agtttcgggc gtagtgcatc gcttgcagac ttcgccgttg
    20581 gctgcgggat atggcttcac aggaattatt gtggcatggc tggcgaaatt aaacccgatc
    20641 gtcattgttt tggtatcagt cctttttggc gcgttgattt tggctgggcg cgagattcag
    20701 ccatcgggtg tcccaaaaat gatccagggc attattctgg tttcactgat tgccagtgac
    20761 tttttactgc ggtatcgtat ccgcatcgtg cgcgcagacg cggaggaata attcaattat
    20821 ggatccaatc atcatacttc aggcaggtgt ggccaccggc acggttttgt tgtttgccac
    20881 cattggtgag atctttgccg aacgttccgg cgttttgaac cttggtgtgg aaggcatgat
    20941 gctgatcggc gcaatgaccg cattcagcac caccatcgca acggggaatc catggcttgg
    21001 agtgttggcg gccatgctgg ctgctggctt gatcagtcag attcatgctt ttatcacgat
    21061 cactttgcaa gccgatcaag ttgtcagtgg cttggcgctc acttttgtag gaacaggtat
    21121 cagtttggtg ttgggcgagg gattgagcaa ggcgggcacg gtttctctca tgccgaattt
    21181 ttcgatcccg ctgctttccc aaattccgtt tttgggtccc atcctgttta caaaacaaag
    21241 tgtgctggtt tacatcggtt atttgctggt gccattgacc tggtattaca tcaaccatac
    21301 gcgccctggg ctgaatttgc gtgcagtggg tgaatacccc tccgccgccg actctttggg
    21361 tattagcgtg ttccgcctgc gctattttta cgttttcatt ggcggaatga tggctggttt
    21421 ggctggagcc accatcagcc ttgcagtggc cccgggctgg ttcagtgaac tgaccacggc
    21481 tgggcaggga tggatcgccg tcgggttggt catttttgcg caatgggatc cagtccgcgc
    21541 gatgtttggt tcgtatgcct ttggggcgct ccgtcgctta attctcgata ttcaaggtcc
    21601 aaccattctc tttgggttca acaatccttt ttactacaac ccgtattggg gctttttcct
    21661 gcaaatgctt ccgtatgcct ttacagttat tgtattggtg attggttcgc gcgaagccat
    21721 tcgcaaacgt ttgggggcgc ctgcagcgct tgggaatcct tatgtccgtg gtgagcgcgg
    21781 caagtgacga gtttctgaca cagctttgat ttataaatcc cgcagggaaa tgcctgcggg
    21841 atttttttgc attgccgtac gtttggcgag aaatactgga caaatgtcac ttgtgacagg
    21901 atgctcaaag tatccataat ggaaaggtca gacgattgtc atccaacgac ttttctctta
    21961 tgagcatcat tcagaccaaa tcggtggcag accaggttga agaaattctt cgggaacgca
    22021 ttcgagatgc gacgtatttg cccggcagcc gaatgccttc tgagagtgag ttatctgatg
    22081 aaatgggggt cagccgtgcc actgtgcgga ccgttctggc aaaattggcg gtcaatggtt
    22141 tgatcattcg gaagcagggg gatggaacgt atgtcaatgc gcgggctcag gaagccagtg
    22201 cccataccgg gaatttatgg gatttggtcc gtctgatcga aaacaacggc tcgaagccgt
    22261 tcattcagcc gatctcaatt acccgtaaac ctgcttctga aaaagaggca caggccctcg
    22321 ccgttgaagc tggagaaccc cttttactgt tcaagcgttt attccttgct gaccaaattc
    22381 cggtcattta ttcaaacaac cttgttccgc tatccttttt tcgtgtatcc actgatgaaa
    22441 ttgacgggca attacacttc cgcgatattc tgcgccaata ctgtcatcag gaaattggat
    22501 ttgtgatcac cgatattcgc tccacactcg ttaatgatga attgcggcat ctgttgagaa
    22561 gcgaactcgg ccccactgtc cttgagttgc aaatgacgtt ttacagccga aatagccagc
    22621 cattggcgct gggattgaat tacttcgatg ataccgccct gcggttgggt ttggttcagg
    22681 catggaatta attcctgaac caaaatattt gaatctgatg tggcgctgag gaggaaggtt
    22741 cgatgcgatc aagattgaac gctttgttcg atgctttgct gccagtgctt gccaccctgg
    22801 cggcacttgg ggtgggggcg atcatgttga ttttcctcaa ggtcaacccg gtgacggcct
    22861 ataaggcgct ttgggatggg gcttttggca gttccaatgc ctttgccgaa actttggtaa
    22921 aggctacacc gttgttgctg gtggcgttgg gaatctgcat ctcatttcgc ggtgatgtga
    22981 tcaatatcgg cggcgaaggg caaatgatca tcggcgcggt tttggccaca tgggtgggtc
    23041 tgacgtttac tggattgccc ggctggttgg ccgtgactct tgctttggtg gctggttttc
    23101 tgggtggggc aatttggggc ggcatcccgg gggtgttgaa ggcatacttc agtgtcaatg
    23161 aaatcttaag caccgtgatg atgaatgcca ttgccgtgca gttgatgaat ttccttttgc
    23221 gcggcccgat gatcgacccc tcgcaggcgg aactggcttc aaaaattccg cagacggcgc
    23281 gtttgttgga tgcattccgc ttgccgcgtt tggcccccac ccgattgcac cttggggctc
    23341 tcattgctgt tttgttggcg gttgctgttt atattttgct ctggcggaca acccttgggt
    23401 atcgcatccg ggcagtgggg caaaacccaa acgcttctcg atatgcaggg atcaaagtgc
    23461 agcgttacat ggtcttggcg cttctgctta gcggggcatt tgcagggttg gctggggctg
    23521 tgcaagttta tggtgtgaac taccgcatga tcaccgatgg ttcttcttca gggtttaccg
    23581 ggtcggcggg gtttaacggc attgtagcgg cgctgtttgg gcaactgcat ccgctttggt
    23641 ccattcctgc gtccatttta tttggggctt tgctggtagg ggcgaacaag atgcaaagag
    23701 tggttcaggt tccttccgca ctggtcattg ctttgaacgg tttggtggtg gtctttgtag
    23761 taagcagtga gatcctccgc aagagacgcc agcgcagaag gctcgcagat gatgaaacgc
    23821 cgcccgtggc ggggtcggct gcaaatcaat ccacacaagc ggagacaggc acatgatctc
    23881 agatctcttt accgtcactg ttctcattgg cattctcgct tctggaattc gccttgcaac
    23941 gccgtatctg tatgctgcca tcggcgaaac cttcagccag aagagcggcg tgttgaattt
    24001 gggcgtggaa gggcagatgc tgctcggtgc ctttgccgct ttttatgttt cactcactac
    24061 agggaattta tggctgggtt tgttgactgc catgttggtg ggggcgttga tggggctggc
    24121 gatggcctat gttacagtca acttgcatgc gcagcagggc atcagcggca ttgggtttta
    24181 tctcttcggt ttgggcatga gtgacctgct cttccaaaag ttgatgggca ccgtggaaac
    24241 ggtcaaaggt tttcctgaaa tctatattcc gggcttgagc gatatccccg gtattggcga
    24301 aatatttttc agccagaaca ttttggtgta tggcgcttat ctgttggtgc cgctcgcgtg
    24361 gttcgtcttg aacaagacca cattggggct aaagatccgt tcggtgggtg agaatcccga
    24421 tgctgccgat tcgttggggg tgagtgtggc gcgtgttcgt tacttcacca tcattcttgg
    24481 cggtgtgctt tcgggcattg ctggcgcgtc gctttcgatc gcgttgttga atgtattcca
    24541 gcaaaacatg accagcgggc tgggcttcat cgccgtggcg ttggtgtatt ttggcggatg
    24601 gcggcctgtg ggggtgctgg gcggtgccct gcttttcagt ttgatcaatt cactgcaatt
    24661 gtggattcag gttttgggga tacccatccc ctctgacatt gcagtgatga tgccgtacgt
    24721 tctgacgatc ctcgtgctgg ttgccacggt atcgagagtc cgctctcctt cggctttgtc
    24781 taaacccttt gaaagggaaa attaatttca gtggatagga ggcaatattt gtccctgtga
    24841 aaatgttttt cgaaagcgta tccgttgtcc cgtttggaaa gaagcgtgtt cattcatcca
    24901 taaacaagga gaagaagatg aaaaaatttg cactgtctct cgtaatcgct tttgtgttgt
    24961 tgctgagcgc atgtggcact cctgctgcca ctgaagcccc tgttgttgag gctcctgcca
    25021 ctgatgctcc cgctgaagtg cccgccgcca ctgaaccgcc tgccgccccc gccgaacctt
    25081 tccgtgtggc tgtggtgatg ccgagcgcca ccaatgacct ggctttcagc cagagcatgt
    25141 atgatgccct tgtgcgcatt caagaagaaa tgggcggccc cgaagccttt gagttcgttt
    25201 tttcggaagg catgttcgtt gtagatgatg ccgctgcggc catccgtgac tatgccacac
    25261 agggatatga cctggtgatc gcacacggtt cacagtacgg ttcatccttg caggaaattg
    25321 cgcctgattt tcccgaaacc agttttgcgt ggggcaccac ggctgatacg tttggtcagc
    25381 ccaatatctt tgcctatgaa gcggcttctc aggaaggcgg ctatgtaaat ggcgtattgg
    25441 ctgccactct ttcgcagagc gcggtgatcg gcgttgttgg cccgatcgag accggggatg
    25501 cgaagttgta tgtggatggt ttcaaggctg gtgttgcagc caccaacccc gcagtgaccg
    25561 tgaatgtgaa ttacatcggt tccttctctg atgtagcgtt ggcttccgaa gcagccacca
    25621 ctcatatttc tgctgatgct gatgtgatga ccggtaccgc ccagatggtt gtgggtgcaa
    25681 ttggcaaagc tgaagaaaat aatgttttgt ggttcggtac gcaatccaac cagtcttcac
    25741 tggctccgaa gatcgtggtt gccagtcagg tgtatcactg ggaagtgacc ctgcgtgaga
    25801 tcattgccct gatcaaggaa ggtacactgg gcggcacttc cttcaaggcc aatcttgcca
    25861 acggcggcga ggtgatcgag ttcaacgccg attatgcgct ttccgatgag gccaaggccc
    25921 ttgctgaaac caccattcag ggaattattg acggcaccat caagatcacg ctgccataat
    25981 agtttaatac ccaggcctga caggtttctg tgaagcgacc tgtcaggcct ccatgagaaa
    26041 taattcagga tggcacccat gactgaatca accaaacttc cttccggtaa accccgcatt
    26101 gagaccatcg aaatgcgcgg aatcaccaaa cgttttcccg gtgtgatcgc caatgacaag
    26161 gccgatttcg atattcacgc gggggaagtc catgctttgc tgggagagaa cggcgcgggg
    26221 aaaagcacgt tgatgaagat tttgtacggc atgtatcgcc cggacgaagg cgagattttg
    26281 ctgaacggaa aaccagtgac cattgcctcg ccaaacgatt cgatcaacct cggtattggc
    26341 atgatccacc agcacttcat gcttgtgcca accctgaccg tggcggagaa tgtggcgctt
    26401 gggttgccat ccactcgtgg ggcgctgact gatttagacc gtgtttcaaa gcggatcgtt
    26461 gaactggctg gcatctatgg tttgaagatt gatccctctg cttatgtctg gcagctttct
    26521 gttgggcagc agcagcgtgt tgaaattatc aaagctcttt atcgcggcgc ggcgctgctc
    26581 atcctcgatg agccgaccgc cgtcctcacc ccgcaggagg tggacgagtt attcgtcatc
    26641 atgcgccaga tggtgaagga tggtcatgcg ctgattttta tttctcataa attgcacgag
    26701 gtggttgaga tcagcaaccg catcacggtg ctgcgtgatg gccgcagaat tggcacacgc
    26761 ccaacctccg aaacgaccaa acaagacctt gccaattgga tggttggccg tgaagttggt
    26821 tttacaccag accgcggcaa ggtggagatc ggggaagccc gtttgaaact tgaaaaggtg
    26881 gaatgtggca gtgaccgtgg gacacccggt ctgcgagggg tcagcattga agtgcgttcg
    26941 ggtgaaattc tgggtattgc gggtgtttcg ggcaacggtc agcgcgagtt ggcagaagcg
    27001 atcaccggtc tgcgcaaagt tactgcaggc agggttttcc ttgaggggaa agacatcacc
    27061 aattttgccc ccggtgatat taccgagcta aatctatcct atatccctga agaacgcatg
    27121 cgcgatggca tgatcaaaga attcaccatt gcggagaata tgatcttgcg cgagcaccac
    27181 aaaatgccgt attcacgcat gggctttttg catttgggtg atatttccaa acatgccgat
    27241 gagttgatcg caaagtttca tgtcaaaacc ccgtcacgtg agacccatgc caaaaatctc
    27301 tcgggtggaa atattcaaaa ggttgtgctt gcacgcgaaa tttcacgcaa cccgcgcgcc
    27361 atcatcgccg cccaacccac acgcggactt gacatcggcg caactgaata tgtccgcgaa
    27421 cagttactgg ttgaacggcg caaaggtgtg gcggtcatgc tcatctccga agaccttgat
    27481 gaaattcttg ctctttctga tcgtatcgcc gtgatttacg aaggccaggt gatggatatt
    27541 gttccgcgtg ccgaagccac ccccgaaagg ttggggttgc tcatggcggg cgtccacccc
    27601 gaggttggtt agctcgtgtc gcttcctgct caagccgccg ggagcattta ttcttattga
    27661 tcaactgtaa cttgatgtaa tgcatccaac ggtatacgaa agtgttcatc aagtagtaaa
    27721 atcgaattcc attggtcggg tggtcgctga tgtgaaatgc atcagcggcg ttgggtcgct
    27781 cagcgcccat cgaaaagtac cgccgcctta gtagaaagga caccacctat gaacctttgg
    27841 caggaataca aacgccccgc ctccgtaacg gaggccattc aagcacttac atctgcatct
    27901 ggccctgcaa tgcccattgc aggcggcaca gatctattgc ttgatttgaa acagggacgc
    27961 catgccccca ttcacacttt aattgacctg accttcgtca cggaaatgac cgctctcgaa
    28021 ttaagaggga atgaactctt tattggagct gccgtcccag tcaaccgcgt cgcactggac
    28081 ccgctaacgg gtacccacgc ccaagccttg gtcgaagcct gtaatttgat cgcaggcccg
    28141 caagtccgca atactgccac gctcggcggc aacgttgccc acgctctccc cgccgcagat
    28201 ggaacgatcg cattgaccgc gctcaacgcg caagctgaag tggctggcct ttcaggtacc
    28261 cgtcgaatgc cattcacatc attgttcctt ggccctggaa aatctgccat cgataaaaca
    28321 aaagaaatca ttgtcggttt ccacttgcca ctttccactc cccactctgc ctcctgcttc
    28381 aagcgcatca tgcgcccgca gggggttgcg cttcctattt tgaattgctc tgtttggctc
    28441 gaaagggatg cggaaatggt caaggatgta cacattgctg tcggcccggg cagttccgtt
    28501 cctttccgcg ccacagacgc cgagaacgct ctgcgtggaa aatccctcaa cgaagaaacc
    28561 ttcaatcaaa ccttggatac cctgctcggg caggctcaat tccgcaccag cgcaagacgc
    28621 gccacggcag attaccgcaa acatatcgtc ggcggcttgt tcaaagatgt gctcgatact
    28681 gcatggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgcg cgagagactt
    28801 cgcctgactg gaaccaagat cggctgtgaa gaagccgagt gcggggcttg taccgtcctg
    28861 gtcgatggac agccgatgat gtcgtgtgtc taccccgcgg agcgtgcgga cggtaaggtc
    28921 gtcaccacga tcgaaggatt ggcgcagcgt gtccacgaag aaatgaaact gcatccgttg
    28981 caggaagcct ttgtggagca tggcgctgtc caatgcggat tctgcatccc cggtcagatc
    29041 atgaccgcgt atgccctgct gaatcgcaac ctagacccga acaacgatga catccgtttt
    29101 gctttgaaag acaccctttg ccgttgtgca ggctatccat ccattgaaaa cgcgatcatt
    29161 gctgcggcgc aggcattgcg cacaggcgag cctgtccaga agccgacgca tattccagac
    29221 tctgtccacg agcataaaac cgtcggacac aaacacctgc gtcccgaagc cgtggagaag
    29281 gtcacaggcg aagccatcta caccgatgac ttgaagttcg acggcatgtt gtatgccaaa
    29341 gccaaacgcg ccatgattcc gcacggcttt ttgaaaaaat tggatacctc gaaagccaaa
    29401 gccttgaaag gtgttatcgc cgttcttacc gccgaagatg tccctgctga aaagaatcac
    29461 ggcctcgtca tttacgattg gcctgtgatg gtgggcgtcg gggaacgcgt gcgctacgtt
    29521 ggcgacgcac ttgccattgt cgccgctgag tcgcctgagt tggccgagca ggcttcggcg
    29581 ttgatcgagg cggagttcga cctccagcct gtgattacga atccagtcat ggctcgcgaa
    29641 gaaggcgtcc cgcaactcca cgagaagggg aacctgctga agcacatcaa ggtccgcaaa
    29701 ggtgatatgg atgcgggttt tgccgaagcc gatattgtta tcgagcacac tttccataca
    29761 cctgccacgg atcatgcttt tatcgagcca gagtgcagca ttgccgtgcc gctgcccgat
    29821 ggacgcatgg aaatctatgt cggttcgcaa attccgtatc aggaccgcac acaggttgcg
    29881 cg
//
LOCUS       DAOQJC010000044        29537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_676_length_29537_cov_8.414592, whole genome shotgun sequence.
ACCESSION   DAOQJC010000044 DAOQJC010000000
VERSION     DAOQJC010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 29537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29537
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_676_length_29537_cov_8.414592"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PLE39_08605"
     CDS             complement(<1..686)
                     /locus_tag="PLE39_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68428.1"
                     /translation="MSRKFFTVLLLAVFMLAQFSVASAATVAGDASITNIPDFANGVD
                     VDIDWAVTGMAAGTKNVLAWSRIQQTPAVNYSTCRLIATINGTSDASGTFTYVLPGGG
                     NGIVLEFIITVDDNDCAGAPTNPVAGDAPMGETTIDSFAPEYFNANPPSTTGLPKTID
                     PVACNTFEYWAMATDHYMFPSTVGYSGIGAWNLQVTGTFAPPAPVGPGEELLSWKFTF
                     PSTASGAWTVS"
     gene            complement(789..2147)
                     /locus_tag="PLE39_08610"
     CDS             complement(789..2147)
                     /locus_tag="PLE39_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNE68429.1"
                     /translation="MSNYKKVDAYLEKNLDQNLEELKRYVAQPSISAQNIGLEECAQM
                     VKEMLEKRGFTAQIKSTGGAPVVFGERKGKSNKTLLIYNHYDVQPPEPLELWESPPFA
                     PEIRDGKMYGRGVSDDKSHLTSRLFALDAILDEDGELPCNVKFIVEGEEETSSVHLHD
                     FVRENMDLLKADACIWEFGGVDHREVPMQYLGLRGICYVELSVESLGTDVHSGLGGSI
                     FPNAAWRLVWALNSLKGQDERILIPGFYDGIIPPNERELELMKKLPDVADEYKKRFGV
                     KEFIKGIKGGNELKIEEVFTPTCTICGITSGYQGPGSKTVQPARASAKVDFRLVPAQM
                     PEDIVKKLRAHLDAQGFTDVKIDFLGGEPAARTNPDDPFIKLIVDTAADIYDAPMELV
                     PTVGGSGPNYPFVHDLGLPVATMGLGHPDTRAHAPNENIRLDLYLKHAKHMARVVKEF
                     SK"
     gene            complement(2419..2979)
                     /locus_tag="PLE39_08615"
     CDS             complement(2419..2979)
                     /locus_tag="PLE39_08615"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNE68430.1"
                     /translation="MSQSKRSNFGINILRALALLTVIGITVFIYSIRERVDEFSHYGY
                     PGIFLITLMANATVILPAPGVAVVFAMGGVFNPFFVGLAAGTGGAIGELTGYMTGFSG
                     QAIIENTKIYDQISPWVQKYGVWAILILSAIPNPFFDLAGIAAGVAKIPLWKFLLFCW
                     IGQIVKMSLFAYAGAYSIDWIVGLYK"
     gene            complement(2957..3379)
                     /locus_tag="PLE39_08620"
     CDS             complement(2957..3379)
                     /locus_tag="PLE39_08620"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE68431.1"
                     /translation="MVIELFADKTPVTVNSFVFLAREGFYDGVIFHRVISNFMAQGGD
                     PTGTGRGGPGYRFQDEFHPSLKHDKRGILSMANAGPGTNGSQFFITHGPTPHLDNRHS
                     VFGQVVDGLDVLMSIPERDPMNVNAPAVKIIRVTIEEI"
     gene            complement(3486..4115)
                     /gene="udk"
                     /locus_tag="PLE39_08625"
     CDS             complement(3486..4115)
                     /gene="udk"
                     /locus_tag="PLE39_08625"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNE68432.1"
                     /translation="MSHEVPLVIGIAGGSGSGKTTVAQEILKRVGADRIAYLQHDSYY
                     KDLTKLPPELRTDGKNFDHPHSLETELLIKHVIALKNREAVEVPIYDFTTDSRMAKTF
                     TVKPQDVILVEGILIFVEAKLRKLFDVKIFVDTDSDLRFIRRLKRDISERGRTTESVI
                     AQYMATARPMHLEFVEPSKRYADVIIPEGGHNVAALDMVVARIESLLKK"
     gene            complement(4189..4392)
                     /locus_tag="PLE39_08630"
     CDS             complement(4189..4392)
                     /locus_tag="PLE39_08630"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNE68433.1"
                     /translation="MVAGGEVKLPLTSFDRSLRLGTGLLFVMVGISTNNWIVGVLGGI
                     VAFMGVYDRCPIWQALTGLFKRS"
     gene            complement(4405..4701)
                     /gene="trxA"
                     /locus_tag="PLE39_08635"
                     /pseudo
     CDS             complement(4405..4701)
                     /gene="trxA"
                     /locus_tag="PLE39_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
     gene            complement(4744..4941)
                     /locus_tag="PLE39_08640"
     CDS             complement(4744..4941)
                     /locus_tag="PLE39_08640"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNE68434.1"
                     /translation="MKRNMSNIDRIVRVVIAALFAYLYFSGIVTGTLGLVLVVLGGVF
                     LLTAIVAFCPLYAPFKFSTYK"
     gene            complement(5015..5689)
                     /locus_tag="PLE39_08645"
     CDS             complement(5015..5689)
                     /locus_tag="PLE39_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE68435.1"
                     /translation="MIDATQFERISHALPILQQADEAFKKEFQRAAFFARIPSGHDVF
                     LEGDRVEAIALLLSGVVRVYKIGETGREITLYRFGNGSSCVLSANAILSQKTFPAIAT
                     VEEDAEAVMIPADTFREWVHRYNLWSDFVFDLLSQRLSTMMAVVDEVAFQRMDRRVAA
                     LILERAQKQNPLPITHQEIASELGSSREVISRLLEDFVNDGSLRSTRGKVEVLDFELL
                     ESRLAM"
     gene            complement(5756..6607)
                     /locus_tag="PLE39_08650"
     CDS             complement(5756..6607)
                     /locus_tag="PLE39_08650"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNE68436.1"
                     /translation="MPKFALVTDSTAYIPTELVKKHNITVVPLILVWGDEMFADGVDI
                     QPEQFYSRLKTAKVMPSTSQATPATMQAIFQPLVDQGMEVLAMFISSKLSGTMQSAIQ
                     AKEMMGSAGEKVTLVDSQCTSMALGFQVLAAARAMDAGASLSECAAIAEKAHEKAGLF
                     FAVDTLEFLHRGGRIGSAQRFIGSALNLKPILALKEGRVEGVERIRTKGKAQDRILEL
                     VAEQVKGKSNIRLAVVHANAPEDAKTLLDRAKQSMNPSEAFVTDLSPVVGTHAGPGTV
                     GLAFMTD"
     gene            6800..7873
                     /locus_tag="PLE39_08655"
     CDS             6800..7873
                     /locus_tag="PLE39_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNE68437.1"
                     /translation="MAFNPINWLLGLFSLDIAIDLGTANTLVHVRGKGIVINEPSWVT
                     VDKKLRQPVAIGLEAKEMVGRTPGNVMVVRPIRDGVIAEFDVTQVMLEYFIGKVHEQS
                     MVPLPRPRVIVGLPTGVTEVEKRAVYDAVMASGARQAMLIEEPIAAALGAGLPVGEIR
                     GSMVVDIGGGTTEVAVMSMSGVVSSRSLRVAGDEMDQDIVQYLRNKYNLLIGEGIAEQ
                     VKWQIGSAYPLQPEKTMEVRGRNLVTGLPETVEVSSVEIREALMGSVQVIIDTVRDAL
                     DEIPPEIVADLMDIGICLAGGGALLQGLAERLTDELKLRVWVAEDSLTCVVRGAAMIF
                     EDLSTFNRYLVGLERGSTRHITG"
     gene            7912..8748
                     /gene="mreC"
                     /locus_tag="PLE39_08660"
     CDS             7912..8748
                     /gene="mreC"
                     /locus_tag="PLE39_08660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNE68438.1"
                     /translation="MNSRSLQTTIIFLVIGGILALAFGGFFGPASRQFSSVLVQFQTW
                     VSSRYLGVQDFFTAPRDIVSLRARNAELESQVSQLQAQVIELQQRVNETEILAALVDF
                     SRSNPESTYKAAAVIGRDPSPFLHYIIINRGSNDDIRRGMPVVTNQGLVGRVDAVIAD
                     AARVQLITDPASAVNTYLQNADTTAVALGSVTGDVSLDMISQDAIVQPGDLILTSGLG
                     GGYPADIPVGQVITLRSLEYELFQQATLQPAVDFTRLSIVLVITNFRPVDISPLEPLP
                     SP"
     gene            8767..9267
                     /locus_tag="PLE39_08665"
     CDS             8767..9267
                     /locus_tag="PLE39_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68439.1"
                     /translation="MRNIVAFPLLVLAVILQSSIVSQVRLLHGYADLILIMLAAWALQ
                     ERVRSSWHWALVACILLGIISKLPWAVSTIGYLAVVFTAQVLQRRVWQAPLLAMFSVT
                     LIGTIFMHLLSYTVLSLSGTPFSFSAVAGSITLPSLLLNIFFSIPIYALMRDLARWVY
                     PSEVYE"
     gene            9264..11468
                     /gene="mrdA"
                     /locus_tag="PLE39_08670"
     CDS             9264..11468
                     /gene="mrdA"
                     /locus_tag="PLE39_08670"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNE68440.1"
                     /translation="MSSNSVPRSVRFEAWRLVTVYVVVVLVFVAFLVRLLRLQVIEGA
                     NWKTSAVDNYTFEVSVPAPRGIIYDRNGYILARNVASYNIIITPANLPDDDSDIQAIY
                     RDLSDLLDVPVGGRADDEALLEEAKLFGPCVPGPGITQLVALQDSLAPYSPVKIKCNV
                     TVEIARIVEEKSVDWPGVTVEIEPIRDYPTGSLTSALVGFLGPIPASLEEEFRAQGFI
                     PNRDKIGYAGVEDSLQEILAGRNGLRVVQVDVAGQELRNLEPPIPAVPGNNVTLTIDT
                     RLQAVAEASLIQEINFWNDYFDTIRISSGVVIAMNPKTGEILAMVSYPTYENNRFARF
                     IPAYYYEQLSQDPRHPLLNNAISAEFPPGSVFKLSTATGSFNEGVIGPDDIVDAPGKL
                     ELCEKFNPNDPCTGLNTRPFVDHIFEKKPEGFGPISFYQCIAYSSNVCFYKLGGGYQD
                     EIEQGLGIERLQQYARALGYDQPSGIELKGEQDGLIPDPKWKRINTGENWSTGDTYIA
                     SVGQGYVLATPLQILMSGATIANNGKLMQPTIIREVTDGDGKPVDIWFNPDPEVFTAT
                     DFEVPGSYQISPFTPNLKWDVTVTPLIQSYSCDAGYCDLNENDLKTIRPESVAAVRAG
                     TRLAVTDPFFGTLYDVFNDFPIAVAGKTGTAEYCDDVARKADQCDFGKWPTHSWTLAY
                     APYDDPEIIVVAFAYHGGEGASVAAPIVSRVIKAYFEFKALDIAQGNPALGQ"
     gene            11563..12243
                     /gene="minC"
                     /locus_tag="PLE39_08675"
     CDS             11563..12243
                     /gene="minC"
                     /locus_tag="PLE39_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNE68441.1"
                     /translation="MEQITSLVQIKGIRDGLLATFSEAPWEDQCLALLTQIDERPAFF
                     QGARLAMDVGTQVLKVNDLVDLRDRLSERNIALWAVISESPTTEQTSQLLGLATRISK
                     PRPEEQRQVADSISDDRALFINKTLRSGTRVEFPGNIVIVGDVNPGAEIVAEGNVVVW
                     GRVRGMIHAGAKGDRSAYICALDLSANQLRIADEVSAMLKPQKDPKPEIATINGEGRL
                     QAELWHVG"
     gene            12266..13063
                     /gene="minD"
                     /locus_tag="PLE39_08680"
     CDS             12266..13063
                     /gene="minD"
                     /locus_tag="PLE39_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNE68442.1"
                     /translation="MTAQVITVTSGKGGVGKTTAVANLATALATDGRKVVCIDGDIGL
                     RNLDVIMGLENRIVYDIVDVIEGRCKLKQAMIRDKHFPDMYLIPAAQTRDKNAVSPSD
                     MVRICNDLRPDTDFIIIDSPAGIERGFRNAIAAADRVLVVTNPEVSAVRDADRVVGIL
                     EAEEKGSPALILNRLNPTLVRNNDMLSPEDVLDLLGIQLIGIVPEDESVIIGSNRGAP
                     VVTDTKSRAGQAFRNIAKRIQGQNVPFMDLEQQGGLWGAIQKLTGRK"
     gene            13065..13316
                     /gene="minE"
                     /locus_tag="PLE39_08685"
     CDS             13065..13316
                     /gene="minE"
                     /locus_tag="PLE39_08685"
                     /inference="COORDINATES: protein motif:HMM:NF001422.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HNE68443.1"
                     /translation="MNFFTRKRSAESAKERLQLVLIHDRTDLTPGQLEELKDDLLKAI
                     SRYIDIDPEAVQIGLERDGRSQRLVADIPLRSASRHRAG"
     gene            13398..14525
                     /locus_tag="PLE39_08690"
     CDS             13398..14525
                     /locus_tag="PLE39_08690"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNE68444.1"
                     /translation="MNREFSWRNFDFILLGAIVLASSFGTAMIRSAVAGNEVLQPLII
                     RQVYFAILGVVLIFLVGSIDYRYWLALYRPIYFGMIAFLVTLTGFGQSAFGAQRWFQV
                     GVLFLQPTEFAKIVAIIVLGRYFEVAQHQPRDLKWAFGAIAWAAGIIIMILLQPNLSN
                     VLLLSVILAVMLWMNGVQVKHVFSAAAIGAAAALSLIVLSVLGISIPGLQPYQQERIV
                     NFVVPDPNDTYGNRYNVQQALIAVGAGGLFGEGYGHGTQTQLRFLKVRHTDFIFAASS
                     EEFGMVGGALIIITLGIIVWRCIRAAQKSRDVAGATIAIGVATLIFFQAAVNIGMNLN
                     LLPVSGLPLPFISYGGSGLTALMLGIGLVESVVMRHKQMEF"
     gene            14609..16090
                     /gene="gltX"
                     /locus_tag="PLE39_08695"
     CDS             14609..16090
                     /gene="gltX"
                     /locus_tag="PLE39_08695"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNE68445.1"
                     /translation="MTTKPARTRFAPSPTGHLHLGGARTALYAYLLAKKTGGQFLLRI
                     EDTDIKRTVPGAEQEIMDGLRWLGLNYDEGPDIGGKYGPYRQTERREIYQAHAKTLVE
                     NGHAYPCFCTPEHLDKVRQEQMKRKENPRYDGTCRVLSADEAAKRIANGEPYTIRFKV
                     PYEGSTVAHDHLRGDITIENKQLNDQVILKTDGMPTYHLAAMVDDHEMQITHVIRGSE
                     WLGTFPLHVNIVRAFGWEEPVWAHLSVFLKPSGKGKMSKRDAPDAMKDGYSVFIKDMQ
                     DLGFTPEGVLNWCALMGWGVAEDDVMTVDQMVDRFTIDSLTPSPAAVNFQRLDHFNAT
                     HIRLFTTEDLAARIKPYFTREGLKVDDGILLKIVPLIRERLTTLDDCLSFGSFFFKED
                     VSPAPADLIAKGLDAKQSAQIAQRAYEILVAQPDISHERCEPPLRAYVEESGLNANQV
                     FGILRVATTGQKVSPPLFESMEIIGREKCLARIKSAIELLEKM"
     gene            complement(16143..17096)
                     /locus_tag="PLE39_08700"
     CDS             complement(16143..17096)
                     /locus_tag="PLE39_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68446.1"
                     /translation="MESSATSVVPEKNTRRKWLELLLDNPVITKELKGRMRGRQGFIL
                     LSAYLALIGFFIGLVYLFLSVDGSLSRNDPGYLQIVGKIIFSTVVLLELLMVSFIGPA
                     LTSGAISSERERQTFDLLRTSLLSGRALVFGKLSSAVVFLLLLIFTAIPIQSMSFFLG
                     GVGMAEMVISIVMLVVSIFFFSALGLFFSSFTKRTLVSTVAAYASIMLSFIVMVLLFF
                     FILVFAGVASSTNTFLSGISEEVLGISVWYLFSTNSFFAAVASEAILVSDQSIFFTTN
                     GMLGTTSLPLPSPWIIYTVFHVILTILLVMASIYFVNRPDK"
     gene            complement(17106..18053)
                     /locus_tag="PLE39_08705"
     CDS             complement(17106..18053)
                     /locus_tag="PLE39_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68447.1"
                     /translation="MSPIIEIKDLCKHFGKRFAVNNISFNVESGEIFGLVGPNGAGKT
                     TTMRMLVTLLRPDKGEIFVGGHSIRKNPNDVRRMIGFMPDSFGVYGDMTVHEYLDFFG
                     ACYKVPPNQRLRLINDLLELVDLGHRRDDMVDTLSTGLKQRLGLARVLIHDPNILVLD
                     EPASGLDPRARVEIRELLLEIARLGKTIIFSSHILADVSELCTRVGIIEDGKLAAIGT
                     LQQLSERVMPHRQLRISLLDRAQEAQINLQSRAGVNEARILENTERPTLEVDFIGDDE
                     AIHQLLAGLLAQGYPVVHFSEETKNLEEVFMRTTRGIVS"
     gene            complement(18050..20431)
                     /locus_tag="PLE39_08710"
     CDS             complement(18050..20431)
                     /locus_tag="PLE39_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68448.1"
                     /translation="MKLPYRSAIYKQTAFLAMIFAAILFTPWTSTTAAAQTQGEITIK
                     AQSGLDNFCKNGTWLPVKVMVENNGKDVDARVQASYKNDMNGNAVYGVDISLPATSRK
                     EFFIYVFLDGTARNFNVSVLDGNKTLAKTALNIACINDQTLLFGLVTDDPSVFAGLNT
                     ISLSGVNAQSAQLKVEDLPDQAQGWEIMDALVISNVDTGKFSAEQKQAMEMWLARGGR
                     LFVTGGTNWQPTAAGLENILPIKLTSTKEVTNLSAISKYSKFPLLTEEQSAVLATGQL
                     LEGATTLVDQSGIPVLVEKQVGFGKVYFFAADPGLEPLNNWVGMLAIYEHLLGFRSPK
                     PVWANGYWDAYQINDALSSMPELALPSFVYICCWLGLYVFVVGPLNYFVLRRIKRPEL
                     AWVSIPILVILFTMLAYFSGYAYRGTRPVLNRLMMAQGWEGVSQSQVNAVIGVYSPSR
                     TTYTVETQEQFMLRPTPITSGNLQGNDDWTSIKTQDGTSMPDIRVEIGGMQVVGATGY
                     LPALTVQSDLTITLGSHVPELSGTITNTGDYTIKDAVLVTASQWSPLGDIAPGQTKKV
                     SQILVNNSNTSVTSQYTIMTTLNIDTYDYYSDVNPEKKRQAAFFQANTNTSNGSIHVN
                     SGIYLMGWVNDEIATPTGLQSQPQEEIDTMLFFERLTPSIKTEAGSLMLTSSIFSWDS
                     SLGDTITTPYYNMSNNGYTLRYQPNQTIRFSKVNTFELNIESTTTPDKVIVSLWNFET
                     QTWDLIPMTFYTIDIPNPQQYIGMDGEVRLNLKGDPNDYVEITAINFTMMVQP"
     gene            20517..21806
                     /locus_tag="PLE39_08715"
     CDS             20517..21806
                     /locus_tag="PLE39_08715"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HNE68449.1"
                     /translation="MPAIEKFQTNSLRSQKYDAEVTRILAAMFQSVEPGAAVKRHLRE
                     NPLPAANRTFVFGLGKAACAMASALTDEIHPTDSLLITKHASPLDLKPATVIEGNHPV
                     PGSLSLAAGAAALKFLSQLTADDLLICLISGGGSALMAAPRIPLVDLQTLTSALLACG
                     ARIDEINILRRHLDQLKGGGIVRHANGARVVSFLLSDVVGDPLEAIASGPTAPDPTTC
                     ADALAVIEKYDLKNKIPSSILHALTETLKPADPLFANVQNTVIASNTIALRTAQVCAQ
                     QEGFQTRVWRSDMQGEAAVLGREMVLELSEMLKNTPRPFCLLAGGEATVTLNSVKSGK
                     GGRNQEFALGAVDELAGMADVLLISLATDGEDGPTDAAGAMVTGESAQRAESLGVSVA
                     DHLSGHDAYPFFEMLGDLVRTGPSGTNVNDIVLCFAF"
     gene            complement(21813..22763)
                     /locus_tag="PLE39_08720"
     CDS             complement(21813..22763)
                     /locus_tag="PLE39_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNE68450.1"
                     /translation="MTKSAFRCLFALALLLVACTSPNPSPTTNPPATNTPSQEQHFIV
                     FGDISDDPAEVIEGTQPIADYMAAKLAAYGITGGHVKVVASTEEMIELLKRGEVDVYF
                     DSTYPATLISDASGAQIILRRWRFGVETYNSVIFASKASGITSIEGLKGKMIAMDTPY
                     STSGFMLPAVYLTENGLKLIGKSESTATVDSNEVGFAFAYDDENILQWVLSGHTPAGV
                     TDDYNFDHAFPPEATAQLVELARTESTPRQVGLARAGLEPELLQAIVQILTTMHEDPN
                     SQTALEKFQTTRFDEFPEGIEAATGRMREMMEIVKDIPLP"
     gene            complement(22795..23403)
                     /locus_tag="PLE39_08725"
     CDS             complement(22795..23403)
                     /locus_tag="PLE39_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNE68451.1"
                     /translation="MTETLLLLFRGFLIGISIAAPVGPIGVLCIRRTLTEGRFKGFLS
                     GMGAASADMVYGAVAAFGLTMITDVLVENKFWFRVIGGCFLIYLGIKTFLEKPADHAA
                     QVKQSGYFGAYLSTFFLTITNPMTILSFAAIFAGTMNIGATSSPMVLVAGVFAGSASW
                     WLALSFGVGLMRDRLTPIHMMWINRLSGIIITTFGAFALAQL"
     gene            complement(23471..24421)
                     /gene="secF"
                     /locus_tag="PLE39_08730"
     CDS             complement(23471..24421)
                     /gene="secF"
                     /locus_tag="PLE39_08730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNE68452.1"
                     /translation="MNIIKNRYIYFLISLLVIIPGIVFMGINWQQTKAGPLQLGIDFR
                     GGSLLEVQFEGERPTAEEISALYSDFSTEEQPIADPVVQSLGESSISIRSKAMTDETK
                     GLIVAEMESRFGSKVTVENFTSVSASVGAEVTQAAGVAILFAAIAILIYIWWAFRSVE
                     HAYRYGTAAILAMLHDVLVVLGVEAILGYYLGWEADALFLTALLTVIGFSVHDTIVVF
                     DRVRENSLLLRKIEYEKMVNHSIVQTLDRSITTQLTVMFTLFALVLFGGDSIRHFVVI
                     LLIGIFSGTYSSIFNAAPILVVWENREWTTWFKPKEETAI"
     gene            complement(24431..25786)
                     /gene="secD"
                     /locus_tag="PLE39_08735"
     CDS             complement(24431..25786)
                     /gene="secD"
                     /locus_tag="PLE39_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNE68453.1"
                     /translation="MRNRNSWLIITLLLVAFSLWIDLSKDIVVYNPFNNTPLVERNVD
                     VKLGLDLRGGLQALLEADVPAETTVSAEELQNATNILQNRANALGVSEITMVTAGERR
                     IVAEFPGLTNPEEMVAALKQTALLEFVDMGSAPVPDGTTILTDFGLETPGQTTNENGE
                     TIYHTVMTGAGLDSAVVQRDPSSGQIAIAFTLDSDAADVFSEYTSSHVGQYLAIVLDK
                     RVISAPRINQAITDGQGQISGGFTQESAQTLAVQLRSGALPIPIKVVQSRTIGATLGE
                     ESIRRSVQAGIIGMIVVILFMAFNYRLPGVIADLALVCYTLFSLMLFKLIPVTLTLPG
                     IAGFILSLGMAVDANVLIFERLKEELRSGRNLRQAIDLAWSRAWPSIRDSNTSTLITC
                     AILFIFGNTFGASIVKGFSVTLSLGVVVSLFTAIIVTRTFLHSVLDSVKTTEHPRWFG
                     L"
     gene            complement(25857..27338)
                     /locus_tag="PLE39_08740"
     CDS             complement(25857..27338)
                     /locus_tag="PLE39_08740"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNE68454.1"
                     /translation="MKKIFLLLMLALLLLPACLPARQATESTPQQNPNPTADPADRSI
                     YKDGLTPAYQSVLNELPTASLYEIHFTIEDDLYHVKGHETVIYTNAEDSALNEVKFRL
                     FPNILGGEMHVDEVDAGGTIVVPNYTLDHSLLTVPLKQPLQPKESITLSMGFTVTVPQ
                     SVELNYGVQAYFNDVLTLAHAYPMIAVYDDEGWNAEIPPQSGDVTYADMSFFNVTVDA
                     PKDLVLVSVGREILRQAERNGNRQTVQYVAGPVRDYYLAASPNYEVVTKESNGVTLRF
                     YAPADQRSGAERTLEIAAKSIEVYENRYAAYPYTELDFVSTPTLALGIEYPGMIAIAD
                     RIVSPYHEYLEGTVAHEVGHQWFYNLVGNDQLDDPWLDESLTQFATLQYFSDQYGQSG
                     YEGFRFSLEGRWARTNNAAIPVGMPVRDYKDIEYGSIVYGRGGLFFEALRDDMGSENF
                     DQFMKAYVNENAWDIATPEKMKQLAEQNCACDLTDLFKSWIYP"
     gene            complement(27335..27700)
                     /locus_tag="PLE39_08745"
     CDS             complement(27335..27700)
                     /locus_tag="PLE39_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68455.1"
                     /translation="MKDEDSTPLIGIVGPCGSGKSTLMAGLETHGYRCRHIAQEHSYV
                     KHMWQVITNPDILIFLHSSFENSTQRRKLNWSLADYEEQQRRLSHARTHAHIIIDTDL
                     LKPDDVLARALTFLKEFTQ"
     gene            complement(27690..28763)
                     /locus_tag="PLE39_08750"
     CDS             complement(27690..28763)
                     /locus_tag="PLE39_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNE68456.1"
                     /translation="MASTAHSHHSGSRKHRWRHFQASIRDTMILLGEFRSAILWFSVA
                     ILGGGILYDFLSRLLNEPVGSLAESIYTVLTITFLQPPNRSFPTHIVLQLFHFLMPII
                     GIIMLAQGLADFGSLLFNRRSRNKEWEMAVASTLKNHIILVGLGHLGYRVALKLHEMG
                     EQIAAIEFNADADTLSVVRGLGIPVIHEDATRPSSLEAANVKSARTIIMASQNDAMNL
                     QIALKARTLNKNIKVVVRIFDEDFAHSLQDQFGFVALSATEMAAPVFAAAASGVDITN
                     PISVEGQLLSLARLTVSEKAPFANKTIGYVEDNYRLNIVLLRHDHQSEMHPTDKSPLH
                     AGDTIAVLGVPEQLSHLLHDNEG"
     gene            complement(28756..>29537)
                     /locus_tag="PLE39_08755"
     CDS             complement(28756..>29537)
                     /locus_tag="PLE39_08755"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE68457.1"
                     /translation="SLVHPNSKNPITCLKQGTDSKIILVEIQIAVAKINKYAVSESGD
                     TLEVVERPTGGLSVVLADGQTSGRGAKAVSMMVVRKVIGLIAEGVRDGAAARAASDAL
                     FADKQGKVISTLNIASVDLHSNTIVLTRNNPSPVFICRGEQVDAHTEESEPLGTSRNV
                     RPVITEVAVEPELAIVIFTDGIVHAGKRRGQPMDVAETLRALIEDQDPSPQQLADSLL
                     AHAVQLDDGRPADDISVLVLKVSPRAGDHVRRMNVRLPFNG"
BASE COUNT         6801 a         7192 c         8087 g         7457 t
ORIGIN      
        1 gacactgtcc atgcaccgct ggcagttgaa gggaatgtga atttccaaga gagcaattct
       61 tcaccggggc caacgggtgc aggcggagca aaggtgccgg taacctgaag attccaagct
      121 ccgatcccag aatacccaac cgtggacggg aacatgtagt ggtcagtagc catggcccaa
      181 tattcgaagg tgttgcaggc tacagggtca atggtctttg gcagacccgt ggtgctgggc
      241 gggttggcat tgaaatactc cggcgcaaaa gagtcgatag tggtttcacc catgggtgca
      301 tcaccggcaa cggggttggt gggagcgcca gcacaatcgt tgtcatctac ggtgatgatg
      361 aattcaagta caataccatt cccaccaccg ggcaggacat aggtaaatgt gccgctggca
      421 tcgctggtgc cgttgatggt tgcgatcaac cggcaggtgc tgtagttgac ggccggggtc
      481 tgctgaatgc ggctccaagc cagaacattt ttggtgccag cggccatacc ggtcacagcc
      541 caatcgatat ctacgtccac tccattggcg aaatcaggga tgttggtgat ggaggcatca
      601 ccagctacag tggctgcgga ggccacactg aactgggcca gcatgaacac ggccaaaagc
      661 aaaacagtga aaaatttcct actcattcgt ttctcctcga aaaatgaatt tcaaactgaa
      721 ttggtctgaa tacgagcatt gtataagcga ttgaggaaca tgtcaattcc gttgcagaaa
      781 ttcgggggtt actttgaaaa ttctttcaca acccgtgcca tgtgtttggc gtgttttaaa
      841 tacaaatcaa ggcgaatgtt ttcatttggc gcgtgggcac gagtgtcggg gtgacccagg
      901 cccatcgtcg caacgggcag gccaaggtca tggacgaacg ggtaattggg gcccgatcca
      961 ccgacagtgg gtacaagttc catgggcgcg tcgtaaatat ccgcggcagt atccacgatc
     1021 aacttaatga aaggatcgtc ggggtttgtg cgtgcggcag gctcgccgcc gaggaagtcg
     1081 atttttacat ctgtgaaacc ctgggcatca agatgggcgc ggagtttctt gacgatatct
     1141 tcaggcattt gcgcaggcac aaggcggaaa tccactttgg cagatgcgcg tgcaggctgc
     1201 acggtttttg agcccgggcc ctgatatccg ctggtgatgc cgcagatggt acaggtgggg
     1261 gtgaacactt cttcgatctt cagttcgttg ccgcctttga tgcctttgat gaattccttt
     1321 acaccaaaac gttttttgta ttcatcggca acatcgggaa gctttttcat caattcgagt
     1381 tcgcgttcat tgggcggaat gatgccatcg taaaaacctg ggatgagaat ccgttcatcc
     1441 tgtcctttga ggctgttcaa tgcccaaacg agtcgccatg ccgcattggg gaagatactg
     1501 ccacccaacc cagagtggac atctgtgcca agtgactcga ccgaaagctc aacatagcag
     1561 atgccgcgca agccgagata ttgcatgggt acttcacggt gatccacgcc gccaaattcc
     1621 caaatgcagg catcggcttt gagaagatcc atattttcac ggacaaagtc atgcaggtga
     1681 acgctgctgg tttcctcttc gccttccacg ataaatttaa cattgcaggg aagttcgcca
     1741 tcttcatcga gaattgcatc cagcgcaaag aggcgtgagg tgagatgact cttgtcatcg
     1801 cttacgccgc ggccatacat tttcccatcg cggatctcag gcgcaaatgg cggtgactcc
     1861 cacaattcca acggttcagg cggctgcacg tcataatgat tgtagatgag cagggtctta
     1921 ttgcttttgc ctttgcgttc gccaaaaacc acaggcgcac cccccgtgga tttgatctgt
     1981 gctgtgaatc cgcgcttttc aagcatctct ttgaccattt gagcgcattc ttccagccca
     2041 atattttgcg cgctgatgct gggttgggcc acgtagcgtt tgagttcttc aagattttga
     2101 tcgaggttct tttcgaggta ggcatctact tttttatagt tactcatgat gaatctcctt
     2161 tgttttttgc tgtgtgcgta aggaaatact aaaaacagaa gtgttcgtat aaactgatgt
     2221 ttcgcggtct gctcgtaatg cgatatttga cctcttgcaa aagtaaagtg ttgtagacca
     2281 caaagtcacc aaggcactaa gtcactaagc ttttttttct ttgagtcttc gagactttgt
     2341 gcctttgtgg tgaaaaaatg gacttatgca agagttctat tcaatatcat ctgaaagtgc
     2401 gggatttatc ccgcactttt atttatacaa gcccacaatc cagtcaatgg aatatgcgcc
     2461 tgcgtaggca aacagactca ttttgacgat ttgaccgatc cagcaaaaga gcaaaaactt
     2521 ccacaatgga atttttgcca cgccagctgc aataccagcc aggtcaaaaa acgggttcgg
     2581 aattgcagaa agaatcaaaa tcgcccagac gccatatttc tgcacccagg gcgaaatctg
     2641 atcatagatc tttgtgtttt caatgattgc ctgaccgctg aaccctgtca tgtaaccggt
     2701 caattcaccg atggcgccgc ctgttcctgc ggcaagccct acaaagaacg ggttgaaaac
     2761 acctcccatt gcaaatacta ctgccactcc gggggctggc agaatcactg tggcatttgc
     2821 catcaaggtg atgaggaaga tccccggata cccatagtgt gaaaattcat ccacgcgctc
     2881 gcggatgctg taaatgaaca cagtgattcc gatcactgtc agcagcgcaa gcgcgcggag
     2941 aatattgatg ccgaagttag atctcttcga ttgtgacacg gatgatcttg accgcggggg
     3001 cgtttacatt catggggtcg cgttcaggga tggacatcaa cacatccaac ccatccacaa
     3061 cctgcccaaa gaccgaatga cggttatcca gatgcggagt ggggccgtgg gtgatgaaga
     3121 actgcgagcc attggttcca ggcccggcgt tcgccatcga aaggattccg cgtttgtcat
     3181 gcttcaggct ggggtggaat tcatcttgaa agcggtaacc ggggccgcca cggccggtac
     3241 cggtcgggtc gccgccttga gccatgaaat ttgagatcac gcggtgaaag atgacaccgt
     3301 cataaaatcc ctcgcgcgcg aggaacacaa agctattcac agtgacgggg gtcttgtctg
     3361 caaacagttc gatcaccatg gtgcctttat cggtttccat gtgtgccttg tactgtttct
     3421 tcgggtcgat ctgcattgcg ggtggggtgc tccattgttt tgccattcca aactctcctt
     3481 tgaatttatt ttttgagcaa cgattcaatt ctcgccacaa ccatgtcaag cgctgcgacg
     3541 ttatgtccgc cttcggggat gatcacatcc gcgtaccgtt tcgaaggttc gacgaattcc
     3601 agatgcatgg gtcgtgctgt tgccatatac tgtgcaatga ccgattcggt tgtgcgcccg
     3661 cgttctgaaa tatcccgttt gaggcggcgg atgaaacgaa ggtcagaatc ggtatccacg
     3721 aatattttca catcgaacag cttccgcagt tttgcctcta caaagatgag aatcccctct
     3781 acgaggatca catcctgcgg tttcaccgtg aaggtcttcg ccattcggct gtctgtggtg
     3841 aaatcataga tgggcacttc caccgcctcg cgatttttga gcgcaattac atgcttgatc
     3901 agcaactcgg tttcaagcga atggggatgg tcgaaattct ttccgtcggt gcgtagttcg
     3961 ggtggtaact tggtcaggtc tttgtagtac gagtcgtgtt gcaggtaggc gattctgtct
     4021 gctccaaccc gtttgaggat ttcctgagcg acggtggttt tccccgaccc cgatccgcct
     4081 gcgatgccga tgactagtgg gacttcatga ctcatggcgg cattatagcc caggaatgaa
     4141 aaaacggctc cgggccaacc cgaagcctgt ttcgctaaag aaagatattt agctgcgttt
     4201 gaacaaacct gtcaatgcct gccagatcgg gcagcgatca tacaccccca taaacgccac
     4261 aattccgcca aggacgccaa cgatccaatt attggtggaa atacccacca tcacaaacaa
     4321 caggccagtt cccagcctca aagaacggtc gaacgaggtg agtggtaatt ttacttcccc
     4381 gcctgcgacc atcgcttcga acaaggtgcg gtacctggct tcattttgcg cgccagtgac
     4441 acgtcccact tctttttcgt tgcggaacat gagcacggtg ggaattccaa acacgcgata
     4501 ctgttcaatg atttcgcgcg cttcatcggc attgactggc atgaactcaa ccctgtcgcc
     4561 atattcctcg gcgagttttt ccaggatcgg tttggtcatc atgcatggtc cgcaccacgg
     4621 cgcccataaa tccacaatga cgggtttccc cgccttggaa agtttttgtt gaaattccgt
     4681 tttattcata agagtaagga caaggcgcgg agcccatttt gttgctccgc gccttgtttt
     4741 agtttatttg taggtgctaa atttgaaggg cgcatacagc gggcagaaag ccacgattgc
     4801 ggtcagcaga aaaaccccgc cgagcaccac aagcacgagg ccgagggttc cggtgacgat
     4861 tccgctaaag tacagataag cgaataaagc ggcgattacg acacggacga tgcgatcgat
     4921 attggacata ttgcgtttca tgataaatct cctttggttg tttgaagtga tgcccccagt
     4981 ataggagatg gccgcaaatc tgtcggtgac aatgtcacat agccaggcgg gattccaaca
     5041 actcgaaatc gagcacttcg acttttccgc gtgtggaacg gaggcttcca tcgttgacaa
     5101 aatcctccag caggcggcta atgacctcgc gcgaactgcc caattctgaa gcgatttcct
     5161 gatgggtgat cggcagtgga ttctgttttt gtgcccgctc cagaatcaac gcggccacgc
     5221 ggcggtccat gcgttggaag gccacttcat ccacaactgc catcatggtg gaaagccgct
     5281 gtgaaagcag gtcgaagaca aaatcgctcc atagattgta acgatgcacc cattcgcgga
     5341 aggtatctgc aggaatcatc acggcttcag catcctcctc caccgtggca atggcaggga
     5401 aggtcttctg gctgaggatt gcgtttgcag aaagaacaca ggatgaccca ttgccaaacc
     5461 gatacaaggt gatttcccgt ccagtttcac cgatcttgta gacccgaacc acaccggaga
     5521 gcagtagggc aatggcttca acccggtctc cctcaagaaa aacatcatgt ccggaaggga
     5581 tgcgcgcaaa aaacgctgca cgctgaaatt cttttttgaa ggcttcatcc gcttgttgaa
     5641 ggatcgggag ggcgtgtgaa atacgttcga attgtgttgc gtcaatcata tctattctat
     5701 ttgagagatg agagcgcttt tatagcgctc tcatgtttta gtttgcaatt gttgcttaat
     5761 cggtcatgaa ggcaagaccg actgtgcccg gtcctgcatg ggtgccaacc accgggctca
     5821 aatctgttac aaaagcctcg gaggggttca tggattgctt cgcacgatcc agcaaagttt
     5881 tggcatcttc aggtgcattg gcatggacca cggccagacg gatatttgat ttacctttca
     5941 cctgttccgc gaccaactca agaatacgat cctgagcctt gcccttggtt cgaattcgct
     6001 ccacgccttc cacgcggccc tctttcaatg cgaggatcgg tttcagattc agcgctgagc
     6061 cgatgaagcg ctgggcactg ccaatacgtc cgccgcgatg caggaattcg agggtatcca
     6121 cagcgaagaa gaggcctgct ttttcatgtg ctttttctgc gatggcggca cattccgaaa
     6181 ggctggcacc agcatccatg gcgcgtgcag cagcgagcac ctgaaagccg agagccattg
     6241 aagtacattg gctatccacc aaagtgactt tttcgcctgc acttcccatc atttccttgg
     6301 cctggatggc agattgcatg gttccagaaa gttttgaaga aatgaacatc gccaaaactt
     6361 ccataccctg gtctacaagc ggttggaaaa tagcttgcat ggtggctggt gttgcctgtg
     6421 aggtggaggg cataaccttg gctgttttta agcggctata gaattgctca ggttgaatat
     6481 cgaccccgtc tgcgaacatt tcatcgcccc ataccaggat caaagggaca acggtgatat
     6541 tatgtttttt aaccagctca gtgggaatat aagcggtact gtcagtgaca agtgcgaatt
     6601 tgggcatgac ttttctccaa acaatggaat atggtgttgc cattgtatcc caacggggag
     6661 attaagtcag gggatgttga agcagatgtc gggtggtgga gaggcgggtt ttcatcctca
     6721 taatcttgct tttggcgggc atggtataat agtactatct agtccgtttt tgaatgaaat
     6781 ttcactttag gagcctattt tggccttcaa cccaattaac tggctgttag gccttttttc
     6841 tctggatatt gccatagacc tcggcactgc taacactctt gttcatgttc gcggcaaggg
     6901 tattgtcatc aatgaaccct cttgggtgac ggttgacaag aagctgcgcc agcctgtggc
     6961 aattggtctt gaggccaagg aaatggttgg ccgtacccca ggcaatgtga tggtggtgcg
     7021 tccgatccgt gatggggtga ttgcggagtt tgatgttacc caggtgatgc ttgagtattt
     7081 cattggcaaa gttcatgagc agagcatggt tccgttgccg cgcccgcgtg tgatcgtggg
     7141 gttgcctaca ggcgtaaccg aagtggagaa gcgtgccgtt tatgatgcgg tgatggcttc
     7201 gggggcacgt caggctatgt tgatcgaaga gccgatcgcg gctgcgctgg gagctggtct
     7261 gccagtcggt gagattcgcg gctcgatggt ggtggatatt ggcggcggta cgaccgaagt
     7321 ggcggtcatg tccatgagcg gtgtggtctc ttcgcgttcg ctgcgtgtgg ctggcgatga
     7381 aatggatcag gatatcgttc aatatcttcg taacaaatac aatttgctga tcggtgaagg
     7441 cattgccgag caggttaaat ggcagatcgg ttctgcatat ccgctccaac ctgaaaaaac
     7501 aatggaagtg cgcggcagga accttgtgac ggggctgcct gaaactgtcg aagtctcttc
     7561 tgttgaaatt cgtgaagctt tgatgggttc tgtgcaggtc atcattgata cggtccgcga
     7621 tgcgcttgat gaaattccgc ccgaaattgt ggccgatttg atggacattg gtatctgcct
     7681 tgcgggtggc ggcgctctct tgcaaggtct ggcagaacgc ttgaccgatg aattgaaatt
     7741 gcgtgtgtgg gtggctgaag actcgctgac ttgtgtggta cgcggtgcgg ccatgatctt
     7801 tgaagacctt tcgaccttca atcgctattt ggttggtttg gaacgcggca gcacacgcca
     7861 cattaccggt taatcgctcc cccggcattg catgcctgag gaaattaaaa tatgaactcc
     7921 cgttctttac aaacgacgat tatctttctt gtcataggtg ggattttagc gttggcattt
     7981 ggcggtttct tcggacccgc ctcccgccaa tttagctccg tgctggttca gtttcaaacc
     8041 tgggtttcct cgcgttatct tggtgtgcag gattttttca ctgcgccgcg tgatattgtt
     8101 tcactgcgtg cccgcaacgc cgagttggaa tcacaggttt cgcagctgca agcgcaggtc
     8161 attgaattgc aacaacgtgt aaatgaaaca gagatcctcg ccgcattggt ggatttttcg
     8221 cgttcgaacc ccgaaagtac atataaagct gctgccgtca ttggccgtga cccaagccct
     8281 ttcctgcatt acattatcat caaccgcggt tccaatgatg atattcgccg tggtatgcct
     8341 gtggtaacca atcagggatt ggtgggccgt gtggatgccg tcattgcgga tgcggcccgt
     8401 gtccaactga tcaccgaccc tgcttcggca gtgaacacct atttgcaaaa tgcggataca
     8461 actgcagtgg ccctgggttc agtgacaggc gatgtttcac tggatatgat ctcgcaggat
     8521 gcaatcgtac aacccggcga tcttatcctg acctcgggcc tgggcggcgg ttatcctgcg
     8581 gatattcccg tcgggcaggt gattacgctg cgcagccttg aatatgaact ttttcagcag
     8641 gccacattgc aacccgcagt ggatttcacc cgcctgtcca ttgttctggt gatcaccaac
     8701 tttcgccctg tggatatttc tccacttgag ccattgcctt ccccgtaaac ttttaaatcc
     8761 aatcacatgc gaaatattgt tgcattccct ttgctcgtgc tggcagtgat tcttcaatcc
     8821 tcaattgtca gtcaggtccg actgcttcat ggttacgcag acctgattct gatcatgctg
     8881 gctgcctggg ccttacagga gcgggtccga tcttcctggc attgggcttt ggtggcctgc
     8941 atcctgcttg ggatcatttc caaattgccc tgggctgtat cgaccattgg ctatttggcg
     9001 gttgttttta ctgctcaggt cttgcaaagg cgtgtgtggc aggctccatt gctggcgatg
     9061 tttagtgtta cactcattgg taccattttc atgcatctgc tgtcttatac agttttgagc
     9121 ctgtctggaa cgccattttc attctcggct gtggcaggtt cgatcacctt gccaagttta
     9181 ttgttaaata ttttcttttc gatcccgatc tatgccttga tgcgcgattt ggcgcgttgg
     9241 gtatatccat cagaggttta tgaatgagta gcaattctgt accacgctcc gttcgttttg
     9301 aagcctggcg gcttgtaacc gtttatgttg tggttgtgct ggtgtttgtg gcttttcttg
     9361 ttcgcttgct cagacttcaa gtcattgaag gagcgaactg gaagaccagc gccgttgata
     9421 actatacctt tgaagtcagt gttcccgccc cgcgtggaat tatctatgat cggaacggct
     9481 atatccttgc acggaatgtt gcctcctaca acatcattat cacaccagca aaccttcccg
     9541 atgacgattc ggatattcag gcgatttacc gcgacctttc tgacctgctg gatgtgcctg
     9601 taggcgggcg cgccgatgac gaggccctgc tcgaagaagc caagttattc ggcccctgtg
     9661 tgccggggcc gggcattact caactggtgg cgttgcaaga ttcgcttgct ccgtacagcc
     9721 cggtcaagat caagtgtaat gtcactgtgg agattgcccg tattgtcgaa gagaaatcgg
     9781 tcgattggcc cggcgtgacg gtcgaaattg agccgatccg tgattatccg acgggttcgc
     9841 taacctcggc gctggttggc ttcttggggc cgatccctgc ttcgctggaa gaggaatttc
     9901 gcgcccaggg ttttatcccc aaccgtgaca agatcggtta tgcaggtgtt gaagattcct
     9961 tgcaggaaat ccttgcaggc aggaatggtc tccgcgtagt gcaagtggat gtagccgggc
    10021 aggagcttcg caaccttgag ccgcccatcc cagctgtgcc gggaaacaat gtaaccctca
    10081 caattgatac ccgtttgcag gcagttgcag aagcctccct gattcaggaa atcaattttt
    10141 ggaacgatta ctttgacacc attcgtattt ccagcggtgt cgtgatcgcg atgaacccga
    10201 agaccggtga aatcctggcg atggtttcct acccgactta tgagaataat cggtttgccc
    10261 gatttatccc tgcttattat tatgaacaat taagtcaaga cccgcgccat ccgttgttaa
    10321 ataatgccat ttccgcagag ttcccgcctg ggtcggtttt caagttgtcc actgcaacag
    10381 gctctttcaa tgaaggtgtg attggacccg atgatattgt ggatgcgcct ggtaaattgg
    10441 aattgtgcga aaagttcaat cccaatgacc cttgtactgg tttgaatacc cgtccattcg
    10501 tcgaccatat tttcgagaaa aagcccgaag ggtttggccc gatcagtttt tatcagtgca
    10561 ttgcctactc aagtaatgta tgcttttata aactgggcgg cggctatcaa gatgaaattg
    10621 aacagggttt gggcatcgaa cgtttgcagc aatatgcccg cgctcttggt tatgaccagc
    10681 catccggtat cgaactaaaa ggtgaacaag atggattgat cccagacccg aagtggaaac
    10741 gcatcaacac cggcgaaaac tggtctacgg gtgataccta tattgccagt gtggggcagg
    10801 gatatgtcct tgccaccccg cttcaaatcc tcatgtctgg agcgaccatt gccaataacg
    10861 gaaagttgat gcaacccacc attatccgcg aagtaaccga tggtgatggc aagcctgtgg
    10921 acatctggtt caaccccgat cctgaggtct tcaccgccac agattttgaa gtgcctggca
    10981 gttatcagat ttctcctttc acccccaacc tcaaatggga cgtgaccgtg accccgctga
    11041 ttcaaagtta ttcctgtgat gcgggatact gtgacctgaa tgaaaacgac ctcaagacga
    11101 ttcgccccga gtcagttgca gcggtacgtg caggtacaag gcttgccgta acagacccat
    11161 tcttcggcac cctttatgac gtattcaacg atttcccgat cgctgttgca ggcaaaacgg
    11221 gaaccgctga atactgcgat gatgtggctc gcaaggccga tcaatgtgat ttcggtaaat
    11281 ggcccaccca ttcatggact ctggcttatg caccctatga cgatcctgag atcattgttg
    11341 tggcttttgc ctatcacggt ggtgaaggtg ccagtgtcgc ggcccccatt gtatcgcgtg
    11401 tcatcaaggc ttatttcgag ttcaaagccc ttgatattgc gcagggaaac cctgctttgg
    11461 gccaataatg accattgtct tcaaattgca ttttacgaac aaccaattca atttgcctga
    11521 cacgtcaggt ataatttgag ccattatttg gtcaaatgtg atatggagca aattacttct
    11581 ctggttcaga tcaaagggat tcgtgatgga cttctcgcga cgttttcaga agcgccgtgg
    11641 gaggatcaat gccttgcctt gttgacgcag attgacgagc gccctgcttt ttttcaaggt
    11701 gcccgccttg cgatggacgt tggtacacag gtcctgaagg tcaacgacct tgtagatttg
    11761 cgcgaccgcc tttcggaacg aaatattgca ctttgggcgg tgatcagcga gtcgccaacc
    11821 actgaacaaa cttcacaatt gcttgggttg gccacccgca tctcgaagcc gcgaccggaa
    11881 gaacaacgtc aggtcgcaga ctcgatctca gatgacaggg cgctttttat caataaaact
    11941 ctccgttcag gcacacgtgt tgaatttccg gggaacattg tcattgttgg cgatgtgaat
    12001 cccggtgcgg aaattgttgc agaaggtaat gtggtggtat ggggcagggt gcgcggcatg
    12061 atccatgccg gggcaaaggg agatcgctcc gcctatattt gcgcacttga tctttccgcc
    12121 aaccaactcc gcattgcaga tgaagtctcg gctatgttga agcctcaaaa agaccccaag
    12181 cccgagatcg ccaccattaa tggtgaagga cgattgcagg ctgaactctg gcacgtcggt
    12241 taaagaatct actttaggaa atgatatgac tgctcaagtg atcactgtta catctggaaa
    12301 aggcggcgtg ggtaaaacca cagccgttgc aaatctcgcc actgctcttg caacagatgg
    12361 caggaaggtc gtctgtattg acggcgatat cggcctgcgc aaccttgatg tcatcatggg
    12421 ccttgaaaac cgtattgtgt acgatattgt tgatgtcatc gaaggccgct gcaaattgaa
    12481 gcaggccatg atacgcgata agcatttccc ggatatgtac ctgatccccg ccgctcagac
    12541 tcgcgacaag aatgcagtct caccttcaga catggtgcgc atctgcaatg accttcggcc
    12601 tgatactgat ttcattatca ttgactcacc cgcaggcatt gagcgtggat tccgcaatgc
    12661 cattgcggcc gctgaccgtg tccttgtggt gaccaacccc gaggtcagcg cagtacgaga
    12721 tgcagaccgt gtcgtgggta tccttgaagc ggaagaaaaa ggcagcccgg cgctcatcct
    12781 caaccgcttg aatccaaccc tggtgcgtaa taacgacatg ctttcccctg aagatgtgct
    12841 cgacctgctt ggaattcaac tgatcggcat cgtacctgag gatgaatctg tgattatcgg
    12901 ttcgaatcgt ggcgccccgg ttgtgaccga tacaaaaagc cgtgcggggc aggccttccg
    12961 taacattgcc aaacgaattc agggacagaa cgtacccttc atggaccttg agcagcaagg
    13021 cggcttgtgg ggtgccatcc aaaaactgac cgggaggaag taagatgaac ttcttcacac
    13081 gaaagcgcag cgccgaaagc gcaaaggaac gtttgcaatt ggtcttgatc cacgaccgca
    13141 ctgacctgac cccaggccag ttggaagaat tgaaagacga cctgctcaaa gcgatttccc
    13201 gctatattga cattgaccct gaagccgtgc agatcggatt ggaacgtgac ggccgctcac
    13261 agcggctggt ggctgatatt cccctgcgga gcgcatcgcg ccatcgtgca ggttgatctg
    13321 taaaaccgta caagcacgga tcagccttgc ggaaacgtct ggttgtatcc gtgctttttt
    13381 gttaactaag gctctctatg aaccgtgaat tttcctggcg aaatttcgac tttattttac
    13441 tgggcgcaat tgtgctggcc tcgtcttttg gcacggcaat gattcgctcc gctgtggcag
    13501 ggaatgaggt cttgcagccg ttgatcatcc gtcaggtata ttttgcaatc cttggcgtgg
    13561 tcttgatttt tctggtcggt tcgattgatt atcgttattg gctggccttg tatcgcccga
    13621 tctactttgg gatgatcgct tttctggtca cattgacagg ctttggccag tcggcttttg
    13681 gtgcccaacg ctggtttcaa gtgggtgtgt tgtttcttca gccaacggag tttgccaaaa
    13741 ttgtcgccat cattgttctg gggcgttatt ttgaggtggc tcaacaccaa ccccgtgacc
    13801 tgaaatgggc ctttggtgcc attgcctggg ctgccggtat catcattatg attttgctgc
    13861 agccaaacct gagtaatgtg cttttgcttt ctgtcattct tgctgtaatg ctttggatga
    13921 acggtgtgca ggtgaagcat gtgttttctg ctgctgccat cggagccgca gcggctcttt
    13981 cgctgatagt cctctctgta cttggcatat ccatccccgg gttacagcct tatcagcagg
    14041 aacgtattgt gaactttgtg gtgccagacc caaacgacac ctacggtaac cgctataatg
    14101 tacagcaggc tttgatcgct gtcggagcag gcggcttgtt tggtgaagga tacggacatg
    14161 gaacccagac ccaattgcgt tttctgaaag tccgccacac cgattttatc ttcgctgcca
    14221 gttctgaaga gtttggcatg gtcggcggcg cgcttatcat tatcacgctg ggaattatcg
    14281 tttggcgctg catccgtgct gcgcagaaat cgcgggatgt ggcaggagct acgatcgcga
    14341 tcggtgtcgc caccctgatc ttttttcaag ccgctgtgaa tattggtatg aacctcaatc
    14401 tgttgccagt ttccggtctt ccgcttcctt tcatcagtta tggcggcagc ggcctcaccg
    14461 ctctcatgct tggaatcggc ctcgttgaaa gtgtcgtcat gcgccacaaa caaatggaat
    14521 tctaaccagc accttcaacc ttaaactttc aacctaaaac ctgtaacctg caatctaaat
    14581 cctgcaaccc tcccaaagga gtccacactt gaccacaaaa cctgcacgta ctcgctttgc
    14641 cccctcgcca acaggacact tacatcttgg cggcgcacgt accgctctgt atgcttatct
    14701 gctcgcaaaa aaaacgggcg ggcagtttct tttacgcatc gaagacactg acataaaacg
    14761 taccgttccc ggcgctgaac aggaaattat ggatggtttg cgctggctgg gtcttaatta
    14821 cgatgaaggt cccgacattg gcggaaaata tggtccatat cgtcaaactg agcgccgcga
    14881 gatttatcag gcgcatgcaa aaacacttgt tgaaaacggc catgcttatc cctgcttttg
    14941 tacgccagag caccttgaca aggttcgcca ggaacagatg aagcgtaagg aaaatccgcg
    15001 ctacgatggg acctgccgtg tgctttccgc tgacgaagcc gcaaaacgca ttgccaatgg
    15061 tgagccatat accattcgct tcaaggtgcc gtatgaaggt tccactgtgg cgcatgacca
    15121 tttgcgcggc gacatcacca tcgaaaacaa acaactcaac gatcaggtca ttctcaaaac
    15181 cgatggaatg ccgacctatc atcttgccgc aatggtggat gaccacgaga tgcagatcac
    15241 acacgtcata cgcggctctg agtggctggg tacctttcct ttgcatgtca acattgtccg
    15301 cgcgtttggc tgggaagagc ctgtttgggc gcatctatcc gtatttctca aaccgagcgg
    15361 caagggtaag atgtcgaaac gtgatgcgcc cgatgccatg aaggatggct attcggtctt
    15421 tatcaaagac atgcaagacc tcggctttac gcctgaaggt gtattgaatt ggtgcgcatt
    15481 gatggggtgg ggcgttgctg aagatgatgt gatgaccgtt gatcaaatgg tggatcgctt
    15541 taccatcgat tcgctgactc cttcccctgc tgcggtcaac ttccaaagac tggatcattt
    15601 caatgcgaca catatccgcc tctttacgac cgaagacctg gctgcccgca tcaagcctta
    15661 ctttacccgt gagggactca aagtggatga cggcattctg ttgaaaattg tgccgcttat
    15721 ccgcgagcgc ctcaccacgc tggatgattg tctatcgttt ggaagtttct tcttcaagga
    15781 agatgtatcg cctgcacccg cagacctgat cgccaaagga ttggatgcga agcaatcggc
    15841 gcagatcgcc caaagggcgt acgagattct cgtggcgcaa ccagatatca gtcacgaaag
    15901 atgcgagccg cctttgcgtg catacgtcga agagagtgga ctcaatgcaa atcaagtttt
    15961 tgggattttg cgcgtagcca cgacagggca aaaggtcagc cctccgttgt ttgaaagcat
    16021 ggaaatcatc ggcagggaga aatgccttgc acgaattaaa tctgcgattg aattgctgga
    16081 aaaaatgtga tcgaaataaa aagctcccgc cgtttctgta tggcgggagc ttttttgttg
    16141 ggctatttat caggacgatt gacgaaataa atgctggcca ttaccagtaa aatcgtcagg
    16201 atgacatgga agaccgtgta aatgatccat ggagatggca gggggagtga tgttgtgcca
    16261 agcataccgt tggtggtgaa gaaaatactt tgatcgctga caagaatggc ttcacttgca
    16321 acggctgcaa agaatgaatt tgtagaaaac agataccaga ctgaaatccc cagaacctct
    16381 tccgagattc ccgataggaa ggtgtttgtg gagcttgcca cacctgcaaa gacgaggata
    16441 aaaaagaaaa gcagaaccat cacaataaag gatagcatga tggatgcgta ggccgccacg
    16501 gtggaaacca gggtgcgctt tgtaaaactt gaaaagaaaa gtcccagggc gctgaagaaa
    16561 aatatggaaa ccaccaacat cacaatggaa atgaccattt cggccatgcc cacaccgccc
    16621 aaaaagaacg acatgctttg gatcgggatg gcggtaaaaa taagcagaag cagaaataca
    16681 actgccgaac tgagtttccc gaataccaga gcacggcctg aaagcagcga tgtgcggagc
    16741 aggtcgaagg tttggcgctc acgctcggag gagatcgccc ctgaggtcaa cgctggccca
    16801 atgaaactta ccatgagcaa ttcaagcaag accactgtgc tgaatatgat cttgccgacg
    16861 atttgcagat aaccagggtc gtttctggac agacttccat ccacgctgag aaagagatag
    16921 accaggccga taaaaaaacc gatcaaggca aggtaggcgc tgagcagaat gaagccctgc
    16981 ctgccgcgca ttctgccttt aagttccttg gttatgacgg ggttatcaag cagaagttca
    17041 agccattttc tgcgggtgtt tttttcaggc acaacagatg ttgcagatga ttccatgatt
    17101 ttcctttaag acacaattcc acgtgtggtg cgcataaaga cttcttcaag atttttggtt
    17161 tcttcgctga aatgcaccac gggatagcct tgtgcgagaa gcccagccaa tagttggtgg
    17221 atggcttcgt cgtctccgat aaaatccact tcgagggtgg ggcgttcggt gttttccaat
    17281 atgcgtgcct cgttgacacc cgctcttgat tgcaggttga tctgtgcttc ctgggcgcga
    17341 tcgagcaggc tgatgcgcag ttgacggtga ggcatgacgc gttcactcaa ttgctgaagt
    17401 gtgccgatgg cagccagttt tccatcttcg atgatgccga cacgtgtgca aagttcgctg
    17461 acatctgcaa ggatatggct ggagaagatg atggttttgc caagcctcgc gatttcaagc
    17521 agtagttcgc ggatctccac tcgtgcgcgc ggatcaaggc ccgaggcagg ttcatccagc
    17581 acgagaatgt ttgggtcgtg gatcaggact cgggcgagtc cgaggcgttg ttttaatcct
    17641 gtggagaggg tatccaccat gtcgtcgcgg cggtgaccaa ggtccacgag ttcgagcagg
    17701 tcattgatca atcgcaagcg ttgattcgga ggtactttgt agcaggcgcc aaagaaatcg
    17761 aggtattcgt gtacggtcat atcgccatat acgccgaaag aatcgggcat gaagccgatc
    17821 atgcggcgca catcgtttgg attctttctg atggaatgcc cgccgacgaa gatttcgcct
    17881 ttatcgggtc tcaacaatgt gaccaacatg cgcatggtgg tggttttgcc tgcaccattt
    17941 ggcccgacca gcccgaagat ttctccgctt tccacgttga aactgatgtt gttaacggca
    18001 aagcgtttgc caaaatgttt gcacaggtct ttgatttcga tgatggggct catggctgca
    18061 ccatcatggt gaaattgatc gccgtgatct caacataatc gttggggtca cctttgaggt
    18121 ttagcctgac ttcaccatcc atgccgatgt attgctgcgg gttggggatg tcgatggtgt
    18181 aaaaggtcat gggaatgagg tcccaggttt gggtttcaaa attccacaaa gagacaatga
    18241 ccttgtctgg cgttgtggtg gattcgatgt ttaattcaaa agtattcacc ttgctgaagc
    18301 gaatggtctg gttgggttga tagcgcaggg tgtagccatt gttcgacatg ttgtagtatg
    18361 gggtggtgat ggtgtctcca agagacgaat cccatgagaa aatgctggaa gtcaacatga
    18421 gcgagccagc ctcggttttg atgctcggcg taagccgttc gaaaaagagc atggtgtcga
    18481 tctcttcctg gggttgtgat tgaagacctg tgggagtggc gatttcatca ttcacccagc
    18541 ccatcaaata tatgccagaa tttacgtgga tgctgccatt gctggtattg gtgttggcct
    18601 ggaaaaaggc ggcctgtctt ttcttttcag ggttgacgtc tgaataatag tcatacgtgt
    18661 ctatattaag cgtggtcatg atcgtgtatt gacttgtcac tgaggtgttc gagttgttta
    18721 caaggatttg actcaccttc ttggtttgcc ctggagcgat atcgcccagc ggtgaccatt
    18781 gactggctgt gaccagaacg gcatccttga tggtgtagtc gcctgtgttt gtaatcgtgc
    18841 cagagagttc agggacgtga ctgccaagcg taatggtcag gtcgctttga acggtaagcg
    18901 caggcaggta acctgttgca ccgacgacct gcattccgcc gatttccacg cgtatgtcag
    18961 gcatgcttgt gccatcctgg gttttgatgg atgtccaatc atcattgccc tgcaggttcc
    19021 cgcttgtgat gggtgtgggc ctgagcatga attgttcctg tgtttccact gtgtaggtgg
    19081 tgcggctcgg cgaatatacc ccgatcacag cgttgacctg tgactggctc acgccttccc
    19141 agccttgagc catcatcagt cgattcagaa cagggcgtgt gccgcgatag gcatacccgg
    19201 agaaatacgc cagcatggtg aacagaatca ccaagatcgg aatggaaacc caggccaact
    19261 cagggcgttt gatgcggcgc aatacaaagt agttcaatgg cccgactaca aatacgtaca
    19321 aaccaagcca gcagcagata taaacaaagg aaggcagggc caattctggc atggaggaaa
    19381 gcgcatcatt gatctgatac gcatcccaat acccatttgc ccaaacgggt ttgggcgatc
    19441 tgaacccaag caaatgctca taaatggcca gcatgccaac ccaattgttg agcggctcca
    19501 ggccagggtc tgctgcaaaa aagtacactt taccaaaacc gacctgtttt tcaaccaaca
    19561 cggggattcc gctttgatca acgagggtgg ttgccccttc cagtaattgt cctgttgcca
    19621 gaactgctga ctgttcttca gttaataatg ggaattttga atatttggat attgccgaaa
    19681 gatttgtaac ttctttggtt gaggtcaatt ttattggcag aatattttcc aaccctgctg
    19741 cagttggctg ccaatttgtg ccgccagtga cgaataacct gccgcctctg gcaagccaca
    19801 tttccatggc ctgtttttgc tctgcgctaa atttgccagt gtccacattg gaaatgacca
    19861 atgcgtccat gatctcccag ccttgggcct gatcgggcag gtcttcgacc tttaattgtg
    19921 ctgattgtgc gttgacgcca gacaacgaaa tggtgttcaa gcctgcaaag acagaagggt
    19981 catctgtcac aaggccaaac aacagtgttt ggtcgttgat gcaggcaatg ttgagggcgg
    20041 ttttggcaag ggttttattg ccatcaagca cgctgacgtt aaagttccgt gcggttccgt
    20101 ccaaaaaaac gtaaataaaa aattccttgc gtgaagtggc tggcagtgaa atatccaccc
    20161 cgtacaccgc atttccgttc atgtcgtttt tgtaggaggc ttgcacgcgt gcgtcaacat
    20221 ccttgccgtt gttttccacc atcactttca cgggtagcca ggtgccgttc ttgcagaaat
    20281 tatccaaccc cgattgcgcc ttgatggtga tctctccctg cgtttgggcg gcggctgttg
    20341 tacttgtcca tggggtgaaa agtattgccg cgaaaatcat cgctagaaag gcagtttgtt
    20401 tgtaaatagc tgagcgatat ggaagtttca agcgttttcc tccaacgggc tgatgatggg
    20461 tgaaatcata tcatatttgg tttattgctt tattgctctg catgtatact tgggtcatgc
    20521 ctgccattga aaaattccag acaaattccc tgcgttcgca gaaatatgat gctgaggtga
    20581 cgcgtatttt ggcagcaatg ttccagtcag ttgagcctgg agcggccgtg aaaaggcact
    20641 tgcgcgaaaa tcccctgccc gcagcaaacc gcacttttgt ttttggattg ggaaaagccg
    20701 cctgtgccat ggcttcagcg ctgacggatg aaatccaccc gacagacagc ctgctcatca
    20761 ccaaacacgc ttcgccgctg gacctgaaac ctgccacggt gatcgagggg aatcatcctg
    20821 tcccaggcag tttgagtctc gccgctggag ccgccgcgtt gaagttcctc tcgcaattga
    20881 cagcagatga cctgctcatt tgcctgatct caggcggcgg atctgccctg atggctgccc
    20941 cgcgtattcc gctggtggat ttgcaaactc tgacctcggc gttgctggct tgtggcgccc
    21001 gcattgacga gatcaacatc ctccgccgac atctggatca gctcaagggc ggcggaattg
    21061 tacggcacgc caacggtgcg cgggtggtca gctttctcct ttcagatgtg gttggtgacc
    21121 ctttggaagc gatcgcctct ggcccgaccg cacccgaccc aaccacctgc gcagacgctc
    21181 ttgctgtcat tgaaaaatac gacctgaaaa acaaaattcc atcttccatc ctgcatgctc
    21241 tgacggaaac cctgaagccc gccgaccctc tctttgcgaa cgtgcaaaat accgtcatcg
    21301 ccagcaacac aattgccctg cggactgcgc aagtatgcgc ccagcaggaa ggatttcaaa
    21361 cccgtgtgtg gcggtctgat atgcaaggtg aagcggctgt tttggggcgt gaaatggttt
    21421 tggagttgag cgagatgctc aaaaatacgc cgcgtccatt ttgcctgttg gcgggtggcg
    21481 aagcgactgt cactttgaat tcggtgaaaa gtggaaaagg gggcaggaat caggaatttg
    21541 ctttgggagc agtggatgag ttggcgggca tggctgatgt tttgttgatc tcgcttgcca
    21601 cagatggaga ggatggtcct acggatgccg caggtgccat ggtgacaggg gaatctgctc
    21661 aaagggctga gtcgcttggg gtgagcgtgg cagaccactt gtcagggcat gacgcgtatc
    21721 cctttttcga gatgctgggt gatctggtca gaactggccc aagcggtacg aatgtcaatg
    21781 acatcgtcct ctgctttgcg ttttgattca aactatggca ggggaatgtc ttttacgatt
    21841 tccatcattt cacgcatgcg ccctgtggct gcttctatgc cttcggggaa ctcatcgaat
    21901 cgtgtggttt ggaatttttc caaagctgtt tggctgtttg ggtcttcgtg catggtggtc
    21961 aggatctgaa caatggcttg cagtaactcg ggttcgaggc ctgcgcgtgc cagtcccact
    22021 tgtcgcggcg tggattcggt gcgtgccaat tccaccagtt gcgcagtggc ctcgggggga
    22081 aatgcgtggt caaaattgta atcatcggtg acgcctgcgg gggtatgtcc gctcaagacc
    22141 cattgcaaaa tattttcatc atcgtaggca aatgcaaagc cgacttcgtt gctgtcaaca
    22201 gttgcggtgc tttcagattt gccgatgagt tttagcccat tttcagtaag atacacggct
    22261 ggcagcataa accccgaagt ggagtatggt gtatccattg cgatcatttt ccccttgagc
    22321 ccttcaatac tggtgatgcc gctggctttg cttgcaaata tgacgctgtt gtacgtttcc
    22381 accccgaaac gccagcggcg caggatgatt tgcgcgcccg aggcatcgct gatcaatgtg
    22441 gcggggtagg tgctgtcgaa atacacatcc acttcgccgc gtttgagcag ctcgatcatt
    22501 tcttcggtgg aggccactac ctttacgtga ccgcctgtaa tgccgtatgc ggcaagtttg
    22561 gcggccatgt aatctgcgat cggctgagtc ccttcaatga cttcggcggg gtcgtctgaa
    22621 atatcgccga agacgatgaa atgctgttcc tgcgaaggag tgttggttgc aggtgggttt
    22681 gttgtgggtg atggattggg gctggtgcag gcaacaagca ggagagcaag ggcgaacagg
    22741 cagcggaatg cgctttttgt catgaacctc atcctgaaac tggatttggt tcgattatag
    22801 ttgtgcgagt gcgaaggctc cgaatgttgt aatgataatg ccagagaggc gattgatcca
    22861 catcatgtgg atgggggtaa gacgatcgcg catcagtcca accccgaaac tgagtgccag
    22921 ccaccaactg gccgaccccg cgaaaacccc tgccaccaaa accatgggtg agcttgttgc
    22981 tccaatgttc attgtccctg caaaaatggc cgcaaaagac agaatggtca tggggttggt
    23041 gatggtcagg aaaaaagtgg aaagatacgc gccaaaatat ccgctctgtt tgacctgtgc
    23101 ggcatggtcg gcgggttttt caaggaaggt tttgattcca agatatatta gaaagcagcc
    23161 gccgatcacc cgaaaccaga acttgttttc aaccaatacg tcggtgatca tggtcaggcc
    23221 aaaagccgca accgcgccat aaaccatgtc ggcactggcg gcgcccatgc cagaaagaaa
    23281 gcccttgaac cttccctctg tcaacgtgcg gcggatgcat agcacgccga tcggcccgac
    23341 gggtgcggcg atggagatgc caattagaaa tcctcggaaa aggaggagca gggtttccgt
    23401 cataaattct ccaatgtttg aattagaatc aaaaaaggac atcgaaactc gatgtccttt
    23461 tttgtaaaag ttagatcgca gtttcttctt tgggcttaaa ccaggttgtc cattcgcggt
    23521 tttcccaaac cactaaaatg ggggccgcat tgaagatgga agagtaggtt ccgctgaaaa
    23581 taccgatgag cagaatgacc acgaagtgac gaatgctgtc gccgccgaaa agaaccagcg
    23641 caaacagggt gaacatcacg gtcaattgtg tggtgatgga acgatccaga gtctgcacga
    23701 tggaatggtt gaccatcttt tcgtattcga tcttgcgaag cagcagggag ttttcgcgga
    23761 cacggtcaaa caccacgatg gtgtcatgca ccgagaaacc gatgacggtg agcagggcgg
    23821 tcaggaagag ggcatccgcc tcccagccga ggtaatagcc aaggatggct tccacaccca
    23881 gtaccaccaa cacgtcatga agcatggcga ggatggcggc ggtgccgtag cggtaggcat
    23941 gttccacact gcggaaggcc caccaaatgt agatcaagat cgcgatggcg gcgaacaaaa
    24001 tagccacacc agccgcctga gttacttcgg cacccaccga agcgctgacg gaggtgaagt
    24061 tttccaccgt cactttggag ccgaagcggg attccatttc agcgacgatc aaacctttgg
    24121 tttcatcggt catcgccttg gaacggatgg agattgaact ctcacccaaa gactgaacca
    24181 cagggtcggc gatgggttgt tcttctgtgg agaaatcgct gtaaagtgca gagatttcct
    24241 ccgcagtggg gcgttcgcct tcgaattgaa cttcaagcag cgagccgccg cggaagtcaa
    24301 tgccaagctg cagcggacca gccttggttt gctgccagtt gatacccatg aagacaatgc
    24361 cagggatgat gaccagcagg gagatgagga agtaaatgta acgatttttg atgatattca
    24421 tgacgcgctc ctagagaccg aaccagcgcg gatgttcggt ggttttgaca ctgtctagca
    24481 cgctgtgcag gaaggtgcgt gtgacgatga tggccgtgaa aagactgacc acaacaccca
    24541 gtgaaagtgt gaccgagaaa cccttcacaa tgctggcgcc aaaggtattg ccgaagatga
    24601 aaaggatcgc acaggtgatc aatgtggagg tgtttgagtc acggatggaa ggccaggcgc
    24661 ggctccaggc gaggtcaatg gcctgacgca ggttgcggcc ggaacggagt tcttccttca
    24721 agcgttcgaa gatcaacacg tttgcgtcca ctgccatacc cagactcaaa ataaaaccag
    24781 cgataccagg cagggtcaat gtgacgggaa ttaacttgaa gagcatcagg gagaacagcg
    24841 tgtagcagac cagggccaga tcggcgatga cacccggtaa gcggtagttg aaggccatga
    24901 acaggatgac cacgatcata ccgatgatac cggcctgaac gctcctgcga atgctttctt
    24961 caccgagggt ggcgccgatg gtgcggctct gaaccacctt gatcggaatg ggcagggcgc
    25021 cagagcgcag ctgcacagcc aaggtttgag ctgattcctg agtaaagccg ccggagatct
    25081 gtccctgacc atcggtgatg gcctgattga ttctcggcgc agagatgaca cgtttatcca
    25141 gtacgatggc gagatactgc ccgacgtggg agctggtgta ttcagaaaat acatcggctg
    25201 cgtcagaatc cagggtgaag gcaattgcga tctggcccga gcttgggtca cgttgaacga
    25261 cggcgctatc caaaccggca ccggtcatga ccgtgtggta gatcgtttcg ccgttttcat
    25321 tggtcgtttg gccgggggtt tcaaggccga aatcggtcag aatggtggtc ccatcgggaa
    25381 caggggcgct gcccatgtcc acaaattcca acagagcggt ctgtttgagg gcggccacca
    25441 tttcttccgg attggtaagg cccgggaatt cagccacaat acggcgttcg ccagcagtca
    25501 ccatggtaat ttcgctgacg ccgagggcgt tcgcgcggtt ttgcaggatg ttggtggcgt
    25561 tttgcagttc ttctgcagag acggtggttt ctgcgggtac atcggcttca aggagtgcct
    25621 gaaggcctcc acgcaggtca aggcccagtt taacatccac gttgcgttcg acgagcggcg
    25681 tattgttaaa tgggttgtag actacgatgt ccttgctgag gtctatccat agcgagaagg
    25741 ctacgagtag aagggttatg attaaccagc tatttctatt gcgcatgttt taactccatc
    25801 cacgaaaatg ccagctttgg gctggcgcgg acgccattat actccgaatg aataatttat
    25861 gggtaaatcc agcttttaaa gagatctgtc aggtcacaag cgcagttttg ttcggcgagt
    25921 tgtttcatct tttcgggggt ggcaatgtcc caggcgtttt catttacata tgccttcatg
    25981 aattggtcaa aattttcaga acccatatca tcgcgcaggg cttcgaagaa gagtccgccg
    26041 cggccgtaaa cgatgctgcc atactcgata tccttgtaat cgcgtacggg catgccgaca
    26101 gggatcgctg cgttgttggt ccgcgcccat cggccttcca ggctgaagcg aaagccttcg
    26161 taacccgatt gcccatattg atctgaaaaa tattgcaggg tggcaaactg cgtgaggctt
    26221 tcatcgagcc acgggtcatc cagttgatca ttgccgacca ggttgtagaa ccactgatgt
    26281 cccacttcgt gtgcaacggt cccttcaagg tattcgtgat acggtgagac gattctatcg
    26341 gcaatggcga tcatgccggg gtattcaatt cccagtgcga gtgtgggtgt ggagacaaaa
    26401 tccaattcgg tgtaggggta ggcggcgtag cgattttcgt acacttcgat ggatttggcg
    26461 gcgatttcaa gtgtgcgttc agcgccagat ctttgatcgg cgggcgcata gaatctaagt
    26521 gtgactccat ttgattcttt cgtgacgact tcgtagtttg gactcgccgc cagataataa
    26581 tcacgcacag gccccgcaac atactggacc gtttgcctgt ttccgttgcg ctccgcttga
    26641 cgaagaatct cccgcccaac actgaccagg accagatcct tcggcgcatc cacagtgaca
    26701 ttgaaaaatg acatgtcggc gtaagtcaca tcgcctgatt gtggggggat ctcggcgttc
    26761 cagccttcgt cgtcgtagac tgcgatcatg gggtaggcgt gtgccagtgt cagcacgtcg
    26821 ttgaaatagg cttgcacacc gtaattcaat tcaaccgatt gcggcacagt gacggtaaac
    26881 cccatgctca gggtgatgga ctctttcggt tggagcggtt gtttcaacgg cacggtgagc
    26941 aggctatgat caagggtgta attggggacg acgatcgttc cgcctgcgtc cacttcgtcc
    27001 acgtgcattt cgccgccgag gatgttggga aacaggcgga atttgacttc atttaatgcg
    27061 ctatcttctg cgttggtgta gatgacggtt tcatggcctt tcacatggta aaggtcatct
    27121 tcgatggtga agtggatttc gtagagactg gctgtgggga gttcatttaa tacgctttga
    27181 taggcaggcg tgagaccatc tttataaatg gaacggtcgg cggggtctgc ggttgggttt
    27241 ggattctgct gcggggttga ttctgttgct tgtctggctg gcaggcaggc gggtaggaga
    27301 agaagcgcaa gcatcagaag caggaaaatc tttttcattg ggtaaattcc tttaagaagg
    27361 tcaaagcccg ggcgaggaca tcgtcaggtt tcaacaggtc ggtatcaatg atgatgtggg
    27421 catgggtgcg ggcatgcgag agcctgcgct gctgctcttc gtaatcggcg agcgaccagt
    27481 ttagttttct gcgttgggtg gagttttcga acgaggagtg tagaaagatg aggatatctg
    27541 gattggtgat gacctgccac atgtgtttga cgtaggaatg ttcctgtgcg atgtggcggc
    27601 agcgatagcc atgtgtttcc agcccagcca taagcgtgga ttttcccgac ccgcatgggc
    27661 cgacgatgcc gatcagcggg gtactgtctt catccttcat tgtcatgcaa caggtgactg
    27721 agctgctcag gcacaccgag gactgcgatc gtatcccccg catgcagtgg acttttatcg
    27781 gtggggtgca tttcagattg gtggtcgtgg cggaggagga cgatgttgag gcggtagtta
    27841 tcttccacgt agccgatggt tttgttggcg aagggtgctt tttctgaaac ggtgagccgc
    27901 gccaggctga gcagttgacc ttccaccgaa atggggttcg taatatcgac acctgaggcg
    27961 gcggcggcaa aaacgggtgc ggccatttcg gtggcgctca gggccacgaa tccgaattgg
    28021 tcttgcaggg agtgggcgaa atcctcatcg aagatgcgga caacgacttt gatgttttta
    28081 ttcagggtgc gggctttgag ggcgatctgc aaattcatgg cgtcgttttg gctggccatg
    28141 atgatggtgc gcgccgattt tacattggcg gcttcgagcg aggatgggcg tgtggcgtct
    28201 tcatgaatga cggggatgcc cagcccacgc acgacgctga gtgtgtcggc gtcggcgttg
    28261 aattcgatgg cggcgatctg ttcgcccatt tcatgcagtt tgagcgcgac acgatagccg
    28321 aggtgtccca gcccgacgag gatgatgtga tttttcaaag tggatgcgac ggccatttcc
    28381 cattccttgt tgcgcgagcg gcgattgaag agcaggctgc cgaaatcggc caatccctgc
    28441 gcgagcatga tgatgccgat gatcggcatt aaaaagtgaa ataattgcag aacgatatgg
    28501 gttggaaaac ttcgattggg cggttgtaaa aacgtgatgg tgaggacggt gtaaatggat
    28561 tcggccaggc tgccgacagg ttcattgaga agcctggaca aaaaatcgta aagaatgcct
    28621 ccgcccagga tcgccacgga aaaccacaaa atggcggagc ggaattcgcc cagcaggatc
    28681 atcgtatcgc ggatggatgc ctgaaaatga cgccagcgat gtttgcgtga acctgaatga
    28741 tgagagtggg ccgtgctagc cattgaatgg gagcctgacg ttcatgcgcc gtacgtggtc
    28801 gcctgcgcgt ggagagactt tcaacaccag cacgctgata tcgtcggcgg ggcggccatc
    28861 atcgagttgt acggcatggg cgaggagcga atcggcaagt tgctgcgggg aggggtcttg
    28921 atcttcgatg agggcgcgca gggtttcagc cacgtccatg ggttggccgc ggcgtttccc
    28981 cgcgtggaca atgccatcgg tgaaaatgac gatggcgagt tcgggttcca cggccacttc
    29041 agtgatgacg ggtcgcacgt tgcgcgaggt gccgagtggt tcgctttctt cggtgtgggc
    29101 atccacttgt tcgccgcggc agatgaagac tggggaagga ttgttgcggg tgaggacgat
    29161 ggtgttgctg tgcaagtcaa cggaagcgat gttgagggtg gaaatgacct ttccctgttt
    29221 gtcagcaaac agcgcatcag atgcggcgcg ggcggcggct ccatcacgga cgccttcggc
    29281 gatcaacccg atgaccttgc gcacgaccat catcgaaacg gctttcgcgc cccgtccgct
    29341 ggtttgaccg tctgccagca caacggagag tccgccggtg gggcgttcca ccacttcgag
    29401 cgtatctccg ctttcagaga cggcgtattt gttgatcttg gcgactgcga tttgaatttc
    29461 cacgaggatg attttactat ctgttccctg tttcaaacag gtgatgggat ttttggaatt
    29521 tggatgaacc agagata
//
LOCUS       DAOQJC010000045        29405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_680_length_29405_cov_9.593458, whole genome shotgun sequence.
ACCESSION   DAOQJC010000045 DAOQJC010000000
VERSION     DAOQJC010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 29405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29405
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_680_length_29405_cov_9.593458"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            403..3090
                     /locus_tag="PLE39_08760"
     CDS             403..3090
                     /locus_tag="PLE39_08760"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNE68458.1"
                     /translation="MTRQELPKAYDFKSTEPRIYAMWETGGFFKPHNDPNKPGFDPSV
                     KPFVISIPPPNVTGELHIGHAMFVSVEDLMIRYHRMKGYSTLWVPGTDHAGIATQLMV
                     ERDLLKNEEVTREELGREKFLERTWEWKKKYGGMITNQIRRLGASCDWDRERFTLDEG
                     LSRAVREAFVRLYEKGLIYRGPRLINWSPGLKTAVSDLEVEYTEENANLYYFKYMIAG
                     SDEFIPVATIRPETILGDTAVAVHPEDDRYRKFIGKKAIVPILGREIPVIADEYVSME
                     FGTGALKITPGHDPNDYAIAQRHNLPIISMLDKAAKINENGGQYKGQDRFEARKNLWA
                     DMKAADLVIKTEPYRTTIPRSQRGGEIVEPMISEQWFVKIESLAQAGLDVVRDGRIRI
                     VPERFEKTYFNWLENIKDWCISRQLWWGHRIPVWYCPENHMTVAREDPTQCATCGSKD
                     IRQDEDVLDTWFSSGLWPFSTLGWPEETPDLKYFYPTSYMETGYDILFFWVARMIMSG
                     LEYTGQAPFHTVYLHGLVRDEHGHKMSKTKGNVIDPLIIMDDLGTDALRFTLLVGSTP
                     GNDTNVGVKKVEANRNFANKIWNAGRFVISAIDGLQPGSELGEYTLADSWIWARLQGL
                     VRDVERQFQNFQYGQAGQQIYDFIWSDFADWYVEIAKQELAEGGARAARAADTLARVF
                     DMALRLLHPFTPFVTEEIWGHLHSALRESPLNEICKDWPDALIVAKFPEPRDPEGWEE
                     SKLAEFALIQEIVRSIRNLRAEKGVSPSKKFAASISAGAKAGLLKEQAQVLASLAALD
                     SAQLTISEALTEKPKDAIALVVGSVEIYIPLAGMVDLANEKARLEKELKEAESHIQRL
                     ENLLGGDFANKAPAALVAKEREKLNAYKDTAEKIKAQLK"
     gene            3196..3459
                     /locus_tag="PLE39_08765"
     CDS             3196..3459
                     /locus_tag="PLE39_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68459.1"
                     /translation="MQNPVQYAACPKCGQSNAKKVSYTFWGGALGPSLMTHVKCQSCS
                     TEYNGKTGQSNQKNIIIYILASFVISFCVCGGLAVLNFALNSN"
     gene            3671..3865
                     /locus_tag="PLE39_08770"
     CDS             3671..3865
                     /locus_tag="PLE39_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNE68460.1"
                     /translation="MTMKIQDDCIACGACVPDCPTESISEGDGTLYVIDQTTCVECEG
                     HFDSPKCVSVCPVDCIVKAE"
     regulatory      3897..4059
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4236..5006
                     /locus_tag="PLE39_08775"
     CDS             4236..5006
                     /locus_tag="PLE39_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE68461.1"
                     /translation="MTKLFNRIPWLGLFSLVAGLLAWDLTARLSGLPRFILPAPVDVW
                     RSFIKALTTGTLLYHTAVTLTEIVLGMLTGVTFATVVGYSLAKSRTLEKVLAPYLVAS
                     QAIPIVAIAPLLVIWLGDGILSKVIICALIVFFPVLVNTIVGVRAVPAALYDLMNSVR
                     ATRWQILFKLELPASLPILLGGLRVGATLSVIGAIVGELVDAQEGLGYLLQLGDFQYD
                     TSMVYVAVFVLIFLALGLYGIVTLLEKRFLKWQSTSSS"
     gene            5112..6092
                     /locus_tag="PLE39_08780"
     CDS             5112..6092
                     /locus_tag="PLE39_08780"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE68462.1"
                     /translation="MLKKLVYLMLGLAIGLSACTSSQGGSEAGTLRKIKLPMGYIPNI
                     QYAPFYVAVEKGYFAEEGIEIEFDYSFETDGVALVGAGELPFAVASGEQVLLARSQEL
                     PVVYAFAWYQQFPISIVSTPELNITTPADLKGQTIGLPGLFGANYIGLQAVLFEAGLT
                     PDDVKMDAIGFNQVEAFATKQKNVVVGYAANEPIVLRSQGMEVVELRVSDYVQLAANG
                     IVTSEMTIKDEPDLVRGMARALAHGIADTIANPEEAYKTSEKFVENLAEQDKDLQMQI
                     LTTSIEFWKAEKIGYSDPSAWENMNDLLVKMGLIPELVDLTKAFTNEFVP"
     gene            6282..7028
                     /locus_tag="PLE39_08785"
     CDS             6282..7028
                     /locus_tag="PLE39_08785"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68463.1"
                     /translation="MTIKPILTVKNISVVFPDDNGGLRALDDISFEVRPQEFICFLGP
                     SGSGKTTLLRILAGLLPPTAGRVNFIRNQHPEIGMVFQQSNLMPWRTVMDNIKLPLEL
                     EGMGETRARIKAREMIDLVGLNGFENSWPRELSGGMAQRVAIARALIHDPDLLLLDEP
                     FASLDALTRERMWMELSRIWQARQKTVIMVTHSINESLFLADRVLVLTQRPGKIKLDV
                     EVDLPRPRKDDIRYTPHFGKLARKLRETIE"
     gene            complement(7008..8333)
                     /locus_tag="PLE39_08790"
     CDS             complement(7008..8333)
                     /locus_tag="PLE39_08790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE68464.1"
                     /translation="MLDVFRPIFSWDYTKKYWLAGVILIAMLCLSPIFHAYFNGNLIF
                     KSDGGAWAIASNLVGGKGYSGCATDYFPNCESTSQATAMREPIPVLLIALARLFHPTK
                     LSALIMQSLYYLGSIPAIYLTLKESSRNVKTALLGTLMWTFSVPVINQVDNGSGDLAA
                     AFFFSFGMYFFLRGYHASKAKDWVISGLFFGLAALSRTVLLGVGIGLGAVMLAKKWFE
                     TRPFSKKQIGGILLFLVSLFLVFSPWVIRNTLVFGQPVIGSSLTGYNIYRMNFIVANA
                     DFQPHYVGAKEGYAAVRDLLSHSTLTGLENEAQLQSIYMKAGLQLITQHPIEYLQLAL
                     YRFLPLWFNVSVNEAYGAGYYMADYLSYVQQFLFLLAVLFGISKHWRSMWPFILALAL
                     GCGAYMAIDAQMRYLVDLMPAVVILSAAGLGSLMPQNEMKINRSLDGLA"
     gene            complement(8528..9007)
                     /locus_tag="PLE39_08795"
     CDS             complement(8528..9007)
                     /locus_tag="PLE39_08795"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HNE68465.1"
                     /translation="MFDHKEKQLADLETAYASFIDTVTGLASEAFFTTLGDWTPRDIA
                     AHFIGWNRITLAGCSALREGAEPFYFYDGTNDYRKVNAKFLSQFSATDRDTLLRDIAA
                     TKEILTAYLKTIPESEWEADTGIIHYRGGPATVARCVDSLIRDYRKHRQEIVDGIPI"
     gene            complement(9020..9439)
                     /locus_tag="PLE39_08800"
     CDS             complement(9020..9439)
                     /locus_tag="PLE39_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68466.1"
                     /translation="MNKPHPVYWVSAAITALCGIAVIGFGIQLVADGQDCKSYADLGC
                     AFRGVFGAVFIPYGALILLLAGLTSLPVRMIRLIGSVLSILAGVLHIGLCCFVASWAL
                     NGTSLKEVSGFLPVPFFIFASAIALLGMGFTGYKSLK"
     gene            complement(9436..9876)
                     /locus_tag="PLE39_08805"
     CDS             complement(9436..9876)
                     /locus_tag="PLE39_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68467.1"
                     /translation="MSLDPNYTQLNRASTERIKQLAASLNDEEMQTRVGEHWTVAIAL
                     AHLAWWDRRVMYVLDMTVKNGKLFVPEIDIIVNDLSLPLWAAIPPREAARIAIESSAD
                     LDARLEVFPQDLLEEIYNYNKRWVVRALHRNEHLNEVDAALKAR"
     gene            complement(9887..10114)
                     /locus_tag="PLE39_08810"
     CDS             complement(9887..10114)
                     /locus_tag="PLE39_08810"
                     /inference="COORDINATES: protein motif:HMM:NF021552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HNE68468.1"
                     /translation="MCRSIKPLRNMDHPVTEQEIQEAALQYVRKVSGYRKPSKANEAA
                     FQKAVDEVAESTRRMLELLKSGSLQVKQFQE"
     gene            complement(10139..10924)
                     /locus_tag="PLE39_08815"
     CDS             complement(10139..10924)
                     /locus_tag="PLE39_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68469.1"
                     /translation="MNPQPKFPTLLHQKTAEFARDFFLNQSTVDTVLVVNSCARGQAT
                     PESDLDMAVLVKPETAHSDMQELETKWQAALQSNPVFQQFKTSHPFMQLHLDLITGQY
                     TPSIWDDGGGPDGFELEVGNQLAYGAPMHEIGSYYSQLQSAWLPYYDPPLQAQRLAMT
                     CHACTYDLDHVPFFFHRGLYFQAFDRLYKAFQEFLQALFISRKTYPIAYNKWIREQVE
                     QKLGLPELYRELPSIISVGNIESEEVIQKSLKLRTLLNKWIVQ"
     gene            complement(10925..>11962)
                     /gene="holB"
                     /locus_tag="PLE39_08820"
     CDS             complement(10925..>11962)
                     /gene="holB"
                     /locus_tag="PLE39_08820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNE68470.1"
                     /translation="PSTCNFQPVTKNPVTQMIDNWNMLGHEWAVDMLRQHAARGEARH
                     AYLFCGPPGLGRRTLALHLAQALNCPHPIAPGVPCGTCRDCRQIEAMQHPDMNVVQAD
                     AEGGTLKVDQVREVQRALSLKPYQAKYRVALFLRFQEANDSAANALLKTLEEAPAHAL
                     LLLTADTPEQLLPTIVSRCEILRMRPLPVKAIESYLLDQGVDEERARLLAHISGGRPG
                     YARKLVDDVTLLEKREEYLNDLQTLIPAPRVEKFSYAEKLAKDKDTMRQTFLIWLSYW
                     RDVMLRVAGAETPLINVDRNMEIEFLAGRLDLPTARRVVSDLESALEKMERNVNSRLL
                     AEILLLDWPKA"
     assembly_gap    11963..12062
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12068..12976)
                     /gene="rsgA"
                     /locus_tag="PLE39_08825"
     CDS             complement(12068..12976)
                     /gene="rsgA"
                     /locus_tag="PLE39_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNE68471.1"
                     /translation="MTNEIQKQGLIIKAQSGFFSVDTGEGLVICQLRGKLKQGRAVGD
                     IAALGDHVLITVLADGSGVVEKVEERKQAIVRLDPRPQGEYQQVLLANADQAVFVFAC
                     ANPNPKLRMLDRFLVVAEKQRIPVLIVANKIDLVEDPHETFGLYESIGYRVIYTSTKT
                     GAGLDELKAQIQNKINALAGPSGVGKSSLLNAIQDGLGLAVNEISTAVNKGRHTTVTR
                     QLFALNNGGYVADTPGWKSLALWDTEAEEMDAYFPELRDLVPHCQFSDCTHKHEPGCA
                     VLAALKAGTIHPERYDSYLRLRAGQE"
     gene            complement(13043..15283)
                     /locus_tag="PLE39_08830"
     CDS             complement(13043..15283)
                     /locus_tag="PLE39_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNE68472.1"
                     /translation="MKVFRWINLIVALALLSSCSSGGAEGNPITDFFVTPTPILPTAN
                     PTIIPAPDVQAAVTSFLQALQKDDFETMYAMLAQQSRDALTLEDFSQRWNTALNEMSA
                     ASIEFAINSSVISPYKAEVGYSVTYKTVLAGDIQRNIVMRLANEENAWKVVWDDSLIM
                     PELAGGNVLKMEYSVPARGDIYDRDGLPIVSQADAFAFGIQTDQIDLEMQGALTAELG
                     KLCGYDPEYIKDQIEASGPGNYLPMCEGTREEAQRLLAISPGGLVISDEYNSRYHFGT
                     GIAPQSVGYTQLISPEQLNSYRRLGYNGSESVGQSGIEKWAEDYLAGQHGGILRIVGP
                     NGAIISTLGQTAAEPADSIYLTLDSNMQKYAQRSIEFFRGAVVVMEVDTGRILAIASS
                     PDFDPNYFEGNNPNAGGTYLLPEGALLNRATQGQYPLGSVFKIITMSAALESGLWIKD
                     TPYDCQYDFTRLDQVMHDWTWQHCQDRIARGDFCDSSDSTPSGLLTLQEGLMRSCNPY
                     FWEIGWDLFTNYSRGTDIANMARAFGLGSPTGIAQLEEEGGQITDPQTEVDAVNQAIG
                     QGDMQVTPLQVARFVAAIANGGTLYRPQIVERIQPIDGDPKLVFRPEANGTLPLRSEN
                     LDILREAMLMVTQNPRGTARFNLRGLQFDVAGKTGTAESGNGKSHAWFAGYTLNEANT
                     NLPDIAIAVIVENIGEGSEYAVPIFRAMVETYYYGSPQRIFYDFGPIGEPPYTPTPFG
                     GGNGLP"
     gene            complement(15298..15969)
                     /locus_tag="PLE39_08835"
     CDS             complement(15298..15969)
                     /locus_tag="PLE39_08835"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNE68473.1"
                     /translation="MINVATLVLASNSPRRRQLLALGNWKFDVIVSDVDETQLTGESP
                     KEYVLRLAQAKALAVAGRSNFEAIIIGSDTSVIDGDEILGKPKDETEAERMLKQLRGK
                     THQVYTGVAVHRVSDSKTLTELCITDVPMRNYSDEEIAAYIRTGDPLDKAGAYAIQHP
                     DFQPVESMRGCYASVMGLPMCHVVRMLQQFGISPSAEVPMACQKLLNYACPVSGAILS
                     GVSNG"
     gene            16209..16781
                     /gene="xpt"
                     /locus_tag="PLE39_08840"
     CDS             16209..16781
                     /gene="xpt"
                     /locus_tag="PLE39_08840"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HNE68474.1"
                     /translation="MQLLKDRILRDGKVLGNGILKVDGFVNHQVDPLLMDACGREFAK
                     RFVNVGATKILTAEISGIAPAVTTAVHMGLPVVYARKNKPITMPDQVYLTLAPSHTKG
                     RMVELIVSPEYLENNEKVLIIDDFLASGATILGLVRLAEAAGSQIVGIGALIEKSFEG
                     GRDALASLGVQVESLANIKSLDNNKVEFVD"
     gene            16870..17154
                     /locus_tag="PLE39_08845"
     CDS             16870..17154
                     /locus_tag="PLE39_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNE68475.1"
                     /translation="MSFLIPVNRLRDTPSLIAFHHPSPGYKFHVLIVPKRQVASLASL
                     DPSDAVFLTDLYSTVQSLVNEFQLKAYRLIVNGGEYQDFPHLHFHLISDT"
     gene            17191..18726
                     /gene="guaA"
                     /locus_tag="PLE39_08850"
     CDS             17191..18726
                     /gene="guaA"
                     /locus_tag="PLE39_08850"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNE68476.1"
                     /translation="MNSIAILDFGSQYAQIIARRIREVNVYCELFPWDAPQEKILSIN
                     PKGFILSGGPKSVYEENAPYIQKFILESGLPIFGICYGMQALTHALGGEVNPSSEREF
                     GFAEIEPLIPNTMLDVISKVWMSHGDKVTRLPQGFMPLAKSGNSPYAAIGDLQRKYFG
                     VQFHPEVNHTPNGTELLKHFAIEICGAKPEWTPASIIDESVKRIREQVGSQRVLAAVS
                     GGVDSSVAAALVHKAIGDQLVCVFVDTGLLRHNEGEQVASAFRGGLGAELITVEASAD
                     FLGALAGVTDPEQKRKIVGEKFIRIFEQQAKNVGQPKFLVQGTIYPDVVESSAPDRNK
                     ADKIKTHHNVGGLPKDMQFELVEPLRYLFKDEVRLVGEALGLSEALVWRQPFPGPGLT
                     VRCLGECTPERVSRLRAADAILLEELSKAGFLGKGAKTSQAFMVLLPVKSVGVMGDYR
                     TYQEAAAIRAVVTDDFMTADWARLPYDLLAKVSSRVVNEVDGINRVVYDVTSKPPATI
                     EWE"
     gene            18796..19692
                     /locus_tag="PLE39_08855"
     CDS             18796..19692
                     /locus_tag="PLE39_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68477.1"
                     /translation="MNFDYGDTLKRAAQMTLRYKSFWVLMTLQMLPSMIMFPLFFYAL
                     WVLENSRGEAGGVWMVVLGAALILLFFASFLLMAYSHSAVTLGVIRVDRGGEITDLME
                     LLRDAFPFYGRSLGLILSIQLTIGMIFTVVFMIIGLLMVVTMGIGAICLQPVILLLTP
                     LSMLLMAFMEAAQTALIAEDLGVLDSLKRALQVVRAHVWKYVIISLVVYFGTSIISGI
                     ISIPLTFPAAILAMSTETMSLSDSQMGMAVMASMFCCFVPVIFAISGVTQTFIKTTLG
                     LSYLRLASPAGEQVITLEEQAS"
     gene            20039..20977
                     /locus_tag="PLE39_08860"
     CDS             20039..20977
                     /locus_tag="PLE39_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68478.1"
                     /translation="MNFNFGEVFTRAWQIIWKHKVLWIFGILASCARGSGGGGGNSGS
                     RYQTSGGDAPFSGGQIEQTFNQIGQYLQDYLWIIVVGIIAILLIGLIFMALGIVGKVG
                     LIKGTYKADADVERLQFAELWSESLPYFWRIFGLNFLLGLAVFVIIIPFVLLSVFTGG
                     IGALCLLPLICILIPVGFVVSIITEQAQTAMVLEDLGIMDGLKRGWEVVKANIVTMIV
                     MVLILGIGGAIVGIVIALPLIFAALPIVIGMGSLGESLTPLYIALACCVVYLPVLVFF
                     NGVITAYTQSVWTLTFMRLTTPKLKEETPVFVEDNA"
     gene            20970..22886
                     /locus_tag="PLE39_08865"
     CDS             20970..22886
                     /locus_tag="PLE39_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNE68479.1"
                     /translation="MRKFFALLFSLGLLFGAASNVRAQQNLASAELYPADASTFPTVS
                     AFLDVFDTNGIFVSGLKPEAVTVMEDGQQLPVDALTEIAVPLQLTVAVNQGPPLDARD
                     STTNISRYQRISEVLVQWAQSRPADLPDDYSLVSQAGPIINHASAADFVVGLSTFQPD
                     FRAATPNLQSLAIALDTVSAQTTRLGMKRAILFITPHTDDINIASALEPYVQRAIENK
                     IRIFVWFVDLETTFTTTSAAAFNNLAIQTGGSMFKFSGLERFPDPEAYFSPLRRVYAL
                     SYTSRLKSVGEHSLSAKVILPGGQLTSAEQRFVLDIQPPNPFPVTNSLQITRRAPEDD
                     PFNTEVLLPFAQEVEIIVEFPDGHPRALTRTTLYVDGVVMDENTVEPFNLFTWNLAAY
                     TTTGEHQIMVEAVDVLGLSKASMPVPVVVTVVQPPKGISALLAKYRTPITFGAILLAG
                     LVLFAILISGRFRLPSIRAAQEARRAEIDPLTQPIRALGPLPTLAEEKNKKRRSIPKK
                     AATDPRSSAEAMASFIRVNADGVPAVAAPIQLFEKEITFGTDPVQCTQILDDPSIAPM
                     HARLRQTEDGGFLLLDNGSIAGTWVNYEPIPREGHRLMHGDMVNFGQLSYRFTLRIPP
                     VNSKPKITVHSIEE"
     gene            22937..23752
                     /locus_tag="PLE39_08870"
     CDS             22937..23752
                     /locus_tag="PLE39_08870"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNE68480.1"
                     /translation="MSGKNNEDRYAVSSFQLSKDDSRPALFAVVADGIGGHRAGEVAA
                     ELAVNYITAVVAESNAKKPISILENAIHHASQSIAAHSAGKEDEDGMGSTCACVWVIE
                     NRLYTAYVGDSRIYLLRGKHIQRLTIDHTWVQEAYEKGIITAEQMHDHPNLHVIRRHL
                     GGIKLPEVDFRLRIDNGETNEESINNQGFHLAPGDTILICSDGLTDLVWDDEILKTIR
                     SKKDMKASAEALINLANERGGHDNITVVMMTMPYLEETGKKKSGILDWLLGED"
     gene            complement(23895..24494)
                     /locus_tag="PLE39_08875"
     CDS             complement(23895..24494)
                     /locus_tag="PLE39_08875"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNE68481.1"
                     /translation="MPTFLLPIFSYLCGSLPFSIWVTRFVKKVDVRDAGSGHATTTNT
                     IRQAGFGWGALVFALDVAKGFLPTYLAIQLGASPLILVLTASFAVIGHCWPVFAQFRG
                     GMGLATTGGVLLAINPLAFLVCFGLLLLLVLLIRHSARASVFAGILAAPALWLLNIRD
                     ISMWAALGMGAVIAVRFLIDWNRKYRELWLDRERGIKKP"
     gene            complement(24500..25066)
                     /locus_tag="PLE39_08880"
     CDS             complement(24500..25066)
                     /locus_tag="PLE39_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68482.1"
                     /translation="MKKIYVLIFAFAFALLISACGSQSVEKSDLSVSCASLQPTENDV
                     KYGLEFGKELFTEVNWQRSYTVKELQSTISWTHRTMSALADVTVYMFCDEVGTDDVTW
                     FYSEDALKIMFEGYDEATLVSSCGKDRLLLYEVDAVEEGQQYKIRLWAEPLNKSRLLS
                     VLLTFPIEESDLLLKYGEEFFPDLPVCP"
     gene            complement(25170..27212)
                     /locus_tag="PLE39_08885"
     CDS             complement(25170..27212)
                     /locus_tag="PLE39_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE68483.1"
                     /translation="MTSRYDILFQPIQIGPVTAPNRFYQVPHCNGFGWRMPEGMAAMR
                     GMKAEGGWGVVCTEETEIHYTSDLSPFFEGRIWSDDDIPAWQLMTEAVHKHGALAGIE
                     LTYNSHDAANLYSRAAAFGPRSAGITGGSGYEPGQSRAASKEDLKQVRKWHRNAAIRA
                     KKAGFDIVYCYAAHNMTLAYHLLSKDNDRSDEYGGSLENRVRFLRELIEDTKDAVGDT
                     CAVAVRFAVDELLGVDGMQHNGEARDIVGMLAELPDLWDVNISNWANDSVTSRFGKEG
                     HQEKYISFVKKLTTKPVVGVGRYTSPDSMVSAIERGIMDLIGAARPSIADPFLPNKIK
                     ENRHEDIRECIGCNICVTGDTRFVPIRCTQNPTMGEEWRRGWHPEIIAPKKSEDEILI
                     VGAGPAGLEAASALGQRGYHVILTDARREAGGRVLLESALPNLNEWRRVVDYRITKIN
                     KTENIFFYPSSPMTAQDILETGTPHIILATGATWRRDGMGRYLTRPVTGNGKIFTPDD
                     LMNHNLPSGKVIIYDDDHYYMGGVLAELLAQPEAGCEVTLITPAPSISYWTQFTLEQD
                     RILKRMHDLNITLLPNHVIASYDPKAATVTNVITSAETTLPCDAIVMVTDRIPNDTLY
                     HELQPALAEGKIKTLRRIGDAEAPNIIAQAIFSGHLAAREFDETIDPDITPFKVPR"
     gene            complement(27222..27854)
                     /locus_tag="PLE39_08890"
     CDS             complement(27222..27854)
                     /locus_tag="PLE39_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68484.1"
                     /translation="MKKQIFILIIAAVILSSCGKPPAPPPTSTPLPPPTITSTPRIVA
                     TNTPLPTVTPVCVSPEPTQQDIDRAHSYTGDAFDSSWDQSEAALEGAYSITWQNIVQG
                     AVAYLEARIKPCGYEEPDLNRDFSEATWSAIFANYESYESIAECKLDSGIRLYQFKTY
                     NLGFEYKINYWVESDTDNRIIVTMIVFPAESQPLLDEYSIRLFPNLPNCS"
     gene            complement(28074..28850)
                     /locus_tag="PLE39_08895"
     CDS             complement(28074..28850)
                     /locus_tag="PLE39_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE68485.1"
                     /translation="MQTKKLNYDKIASEYNQRYPSTQFWERGQALLELAKRTKSQRIL
                     EAGSGTGFWLNLLHQVTPQLYGMDYSAGMIAQARQQPAPIQLARGTATQLPYQNETFD
                     LVYCVDAIHHFGDHAAFVNEAFRILKPGGTLAVIGHDPHSGDGSWYIYEYFDTVYETD
                     LSRYPSARTIEILMDQAGFRGFQMQNVEHIQNLHVGKAVLNDPFIKHNATSQLALLSE
                     ETYQAGMDKINARLDQAQLENQQLIFKSDFWVRMFTGIKP"
     gene            complement(28889..29086)
                     /locus_tag="PLE39_08900"
     CDS             complement(28889..29086)
                     /locus_tag="PLE39_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HNE68486.1"
                     /translation="MNGQTRANIKPGITVLIVLKQDQRTGKLTKGVVKDILTKSPNHP
                     HGIKVRLMDGQVGRVKEIVPV"
     gene            complement(29083..>29405)
                     /locus_tag="PLE39_08905"
     CDS             complement(29083..>29405)
                     /locus_tag="PLE39_08905"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNE68487.1"
                     /translation="KMTNPPTRPVRYVVIHKPGPKWQYGIDYRQQDGVGEHVEHYLKF
                     HQQGKLQLGGPFLLPDVGGMMVTTKEVSQEEIEAFAAADPAVKSGLLIFEVRPWMTPM
                     EHEQ"
BASE COUNT         6593 a         7237 c         8175 g         7300 t
ORIGIN      
        1 attcttgacg ttcccccctt cggcgcgtat aatcccgctc attcaggagt agtaagcgaa
       61 acaagcctga cagagaagga tgcatgggtc aagttctgaa aggaatcgag accgaaatgg
      121 tggaagcatc cacaggaact gattgctgaa gtcgtctgat gggtgtgccg aagaaaacat
      181 tgttcaatga gcagtggtga gtagaacggc acgggtatgc ccgttatagc attcaagtgc
      241 gtttgtcgaa agataaacga attgaggtgg taccgcgaag caatacttcg tcctcatgtt
      301 ggatgaagta tttttatttt aaatgttggc tgaaaacggg tagataaggg acaggtatgc
      361 aaatttccat gcaggcctgt ttacctcaca caaggagaaa gaatgaccag acaggaattg
      421 ccgaaagcct acgatttcaa atccaccgaa ccgcgcattt atgcgatgtg ggaaacgggc
      481 ggattcttca agccgcataa tgaccccaac aaacctggtt tcgacccaag cgtcaaaccc
      541 tttgtcattt cgatcccgcc gcccaatgtg acgggtgaat tgcatatcgg tcatgccatg
      601 ttcgtcagcg tggaagacct gatgatccgc tatcaccgca tgaaagggta ttccacgttg
      661 tgggtgccag gcaccgacca tgcaggcatt gccacccagc ttatggtgga acgtgatctg
      721 ttgaaaaacg aagaagtgac ccgcgaagaa ttgggccgtg agaaattcct cgaacggacc
      781 tgggagtgga agaagaaata cggcggcatg atcacaaacc agattcgacg cctgggcgcc
      841 tcctgcgatt gggatcggga acgcttcact cttgacgaag gcctgagtcg ggcagtccga
      901 gaggcatttg tgcgcctgta tgaaaaagga ctgatctatc gcggcccgcg cctgatcaac
      961 tggtcgccag ggttgaagac cgctgtttca gacctcgaag tggaatacac cgaagagaac
     1021 gccaacctgt attacttcaa gtacatgatc gctggctccg atgaattcat ccccgttgcc
     1081 accatccgtc ctgaaacgat tcttggggat accgctgtag cagttcaccc tgaggatgac
     1141 cgttacagga aattcatcgg taaaaaggcc atcgtgccga tcctcggacg tgaaatccct
     1201 gtgatcgccg atgaatatgt cagcatggaa tttggtacgg gcgcgctgaa gatcactcca
     1261 gggcacgatc cgaacgacta cgccattgca cagcggcata atttgccgat catttccatg
     1321 cttgataagg cggcgaagat caatgaaaac ggcggccagt ataaaggtca ggaccgattc
     1381 gaggcgcgca agaatctgtg ggccgatatg aaagccgcag acctggtcat caagaccgaa
     1441 ccgtaccgca cgaccattcc acgctcacag cgcggcggtg agatcgttga gccgatgatc
     1501 tccgaacaat ggttcgtgaa gatcgaatcg ttggcgcagg cagggttgga cgtggtgcgc
     1561 gatggacgaa tccgaatcgt gcctgaacgc ttcgaaaaga cctacttcaa ctggctggaa
     1621 aacatcaaag attggtgtat cagccgccag ttgtggtggg gacatcgcat ccctgtgtgg
     1681 tattgccccg aaaaccacat gaccgtggca cgcgaagacc cgacccaatg tgccacctgc
     1741 ggttcaaagg atattcgtca ggatgaagat gtgttggata cgtggttttc atctggcttg
     1801 tggccttttt ccacactggg ctggcccgag gaaacccccg acctgaaata tttctatccc
     1861 acctcctaca tggaaacagg atatgacatt ctgtttttct gggtagcgcg catgatcatg
     1921 agtggacttg aatacaccgg acaggcgccc ttccacacag tgtacctgca tgggctcgtc
     1981 cgcgatgaac atggacataa aatgtcgaag accaaaggca acgtgatcga cccgctcatc
     2041 atcatggatg atctcggtac cgatgcgtta cgctttacgt tgttggtggg gtccacccct
     2101 ggaaatgaca ccaatgtggg cgtgaagaaa gtggaagcca atcgtaactt tgccaacaag
     2161 atctggaacg cgggcaggtt tgtgatcagc gcgattgacg ggttgcagcc cgggagcgag
     2221 ctgggcgaat ataccctggc cgattcatgg atctgggccc gcctgcaggg actcgtccgc
     2281 gatgtggaac gccagttcca gaacttccaa tatgggcagg ctggtcagca gatctatgat
     2341 ttcatctggt ctgattttgc agactggtat gtggaaattg ccaagcagga attggccgaa
     2401 ggcggtgccc gcgcagccag agccgctgat acattggctc gtgtcttcga tatggctttg
     2461 cgtctgctgc atccctttac cccctttgtg accgaagaga tctgggggca cctgcacagt
     2521 gccctgcgcg aatcgccgtt gaatgagatc tgcaaagact ggcccgatgc cctcatcgtt
     2581 gccaaattcc ccgagccgcg tgaccccgaa ggctgggaag aatccaagct ggctgaattc
     2641 gctttgattc aagagattgt ccgctcgatt cgaaatctgc gtgcggagaa gggtgtatct
     2701 cccagcaaga agttcgccgc gagcatctcg gcaggcgcaa aagcaggttt gctcaaagag
     2761 caggcacaag tgctcgcatc tcttgcggcc ttggattctg cccagctcac catcagcgaa
     2821 gcgttgactg aaaagccgaa ggatgccatt gcccttgtgg ttggttctgt cgagatctat
     2881 attccgcttg caggcatggt ggatcttgcc aacgaaaagg cccgcctaga aaaagaactc
     2941 aaggaagccg aatcgcacat tcagcgtttg gagaatctgc tcggcggcga ttttgccaac
     3001 aaagcccccg ccgcgctggt ggcaaaagaa cgtgaaaaat tgaatgccta taaagatacg
     3061 gcagaaaaga tcaaggcgca gttgaaataa aaacagtagg gacatggcgc cgccatgtcc
     3121 ctaccaatgg ttgattgtcc gatccctcac tccaaataaa atatcaccca attcatttta
     3181 tttggaggaa ccttcatgca aaacccagtt cagtacgctg cctgtcccaa gtgtggacaa
     3241 agcaatgcaa agaaagtcag ttacaccttt tggggcggcg cactcggccc atctttgatg
     3301 acccatgtta agtgccagag ctgcagcaca gaatacaatg gcaaaaccgg gcaatcaaac
     3361 cagaagaaca ttattatcta catccttgcg agttttgtga tctcattttg tgtgtgcggc
     3421 ggtttggctg ttttgaattt tgcactgaac agtaactaac tgacactatt ttagtaagaa
     3481 gttcattgcc gcgaattaca ggagtctttg atataccttt cctgtggttc gcggcgatga
     3541 tttttaaata cgttttggat aatgttacaa aagcctgaaa agaaaagaca atagtcaatt
     3601 gactgggaaa atcatggggt gtatcttcgt ggggcaataa attcaaaccc agaaaaggaa
     3661 tctctgaacc atgaccatga aaatccaaga tgactgcatc gcctgcggtg catgcgtacc
     3721 cgattgcccc accgaatcca tctcagaagg cgatggcacc ctgtatgtca tcgaccagac
     3781 cacctgtgtt gaatgtgaag gccattttga ttcacccaaa tgcgtctctg tttgcccggt
     3841 cgattgcatt gtcaaggcgg agtaacctgc aggtggtata atttttacaa caaaataata
     3901 accttcgggg cagggtgaca cttgcaccac actggcaggt gaattcccga tcggtggtaa
     3961 agcccacgag cctgtatgac cgacttaagt cggtcgtaca gagcatgacc cggtgtaatt
     4021 ccggggccga cagtatagtc tggatagaag aaggtagagc aggcctgtgc aggtactgtg
     4081 tagattcatg ccccgaaaac gaacgttgtt ttcgggcttt ttttctggat gtgaatccgc
     4141 atcacgcttc gcgccgccct ggcttgcttc acgtgccccg cagttgattc tggcgtgggc
     4201 atttttttgt tccgctgcct tgtggaaaac ctgcaatgac aaaactcttt aaccgtattc
     4261 cctggcttgg attgttttcc ctcgtggctg gcttgctggc atgggatttg accgcgcgtc
     4321 tgagcggatt gcctcgtttt attttgcccg cccccgtgga tgtgtggcgg agcttcatca
     4381 aagcgttgac cactggaacg ctgttgtatc acacggcggt cacgctgaca gagatcgtgc
     4441 ttggcatgtt gacaggcgtg acctttgcca cggttgttgg ttactcactg gcgaaatcac
     4501 gtacgctgga gaaggtgttg gccccgtatc ttgtggcgag ccaggccatt ccgatcgtgg
     4561 cgatcgcgcc tttgttggtg atctggctgg gagatggaat cctctcgaag gtgatcatct
     4621 gtgcgttgat cgtgttcttc cctgtattgg tgaataccat tgtgggggtg cgggcagtcc
     4681 ctgccgcact gtatgacctg atgaattccg tccgcgccac acgctggcag atccttttta
     4741 agttggaact gcctgcatcc ctgccgattt tgctgggcgg attgcgtgtc ggcgcgactt
     4801 tgtctgtgat cggggccatt gtcggcgagc tggtggatgc acaggaaggg ctgggatacc
     4861 tgttgcagct gggcgatttt caatatgata cgtccatggt gtatgtggcg gtgtttgttc
     4921 tgatcttcct cgcacttggg ttgtatggaa tcgtaaccct gttggaaaaa agattcttga
     4981 aatggcaaag cacttcgtcc tcttgatgag gcatcgcagt tgaagaataa aaaatgaacc
     5041 gcaaagctcg caaagaagag caagaaaacc tggcgatctg cgcggtaaga aaaaaatata
     5101 ggagacgtaa tatgttgaag aaattagttt atctcatgct tgggctggcg atcggccttt
     5161 cggcctgcac cagttcacag ggcggaagtg aagcgggcac gcttcgcaaa atcaaactgc
     5221 cgatggggta cattcccaat attcagtatg cgccgtttta tgtcgcagtc gaaaagggat
     5281 attttgcgga agaaggcatt gagatcgaat tcgattattc ttttgagacc gatggcgtgg
     5341 cattggtggg tgcgggggaa ctgccttttg ctgtggcttc gggtgaacag gtgctgctgg
     5401 cccgttcgca ggaattgcct gtagtctatg cgtttgcgtg gtatcagcaa tttcccattt
     5461 cgatcgtatc cacccctgaa ttgaacatca cgacccccgc tgatttgaaa ggacagacca
     5521 ttggcctgcc tggtttgttt ggcgcgaatt atattggttt gcaggccgtg ttgtttgaag
     5581 caggcctcac acccgatgac gtaaagatgg atgcgatcgg gtttaatcaa gtggaggcat
     5641 ttgccacgaa acagaagaat gtagtggtgg ggtatgccgc aaatgagccg atcgtcctgc
     5701 gttcgcaagg catggaggtt gttgagttgc gggtttcaga ttatgtgcag cttgccgcga
     5761 acggaattgt caccagcgag atgacgatca aggatgaacc cgatcttgtg cggggcatgg
     5821 cgcgggcgct ggcacacggc attgccgata ccattgccaa ccctgaagag gcatacaaga
     5881 ccagcgaaaa atttgttgaa aaccttgccg aacaggataa ggatttgcag atgcagatcc
     5941 tgacgacatc cattgagttt tggaaggccg agaagatcgg ttactcagac ccaagtgcct
     6001 gggaaaatat gaacgacctt ttggtgaaaa tgggcttgat ccccgaactg gtggacctga
     6061 ccaaagcctt caccaatgag ttcgtgccgt aatgtagagg ccgattctcg attctccgtg
     6121 atcactcacc ttgcgaagtt gactggatac ggcgcgaaat ttgccaaagg tgtaaccgcg
     6181 gtctgccgcc tgcggtcagg tatttttcaa agcttgtttc atggcgctgc gtaaggtgag
     6241 taactcatcc tttcaggaat tggcatcgga agttggaaac catgaccatt aagcccattc
     6301 ttactgttaa aaatatttcg gttgtctttc cagatgataa cggcggtttg cgcgcgttgg
     6361 atgatatttc attcgaggtg cgcccgcagg aattcatttg ttttcttggg ccgtcggggt
     6421 ctggtaagac cacactgctg cgcatcctgg cggggctttt gccgcccaca gcggggcggg
     6481 taaatttcat tcgcaaccaa caccctgaga tcggcatggt ctttcagcaa tcgaatctca
     6541 tgccgtggcg aacggtgatg gacaatatta aattaccgct tgagttggaa gggatggggg
     6601 agacccgggc acgcatcaag gcgcgtgaaa tgatcgatct ggtcgggctg aatggatttg
     6661 aaaactcctg gccgagggaa ttatctggcg gaatggcaca gcgggtggcg atcgctcgcg
     6721 cgctcatcca tgacccagac ctgctgctgc tcgatgagcc ttttgcttcg ctcgatgccc
     6781 tcacccgcga acgcatgtgg atggaacttt cgcgcatctg gcaggcgcgc cagaaaaccg
     6841 tcatcatggt gacccattcc atcaacgagt cgctcttcct tgcagaccgc gttctggtgc
     6901 tgacgcagcg ccccggtaaa ataaaactgg acgtggaggt tgacctgcca cgtccacgca
     6961 aggacgatat ccgttacacg ccgcattttg gaaaactggc gcggaaatta cgcgagacca
     7021 tcgagtgatc ggttgatctt catttcattt tgaggcatta aacttcccaa gccagccgcc
     7081 gaaagaatga caactgcggg catgagatcg accagatagc gcatctgggc gtcaatggcc
     7141 atgtatgctc cgcatcccaa agcaagcgca aggataaacg gccacatgct gcgccagtgt
     7201 ttcgaaattc caaacagtac ggccaggaga aacaagaact gctgaacgta cgagagatag
     7261 tctgccatgt agtaacccgc gccatatgct tcgttgacgc tgacgttgaa ccataaaggc
     7321 aggaagcggt acagcgccaa ttgtaaatac tcaatggggt gctgggtaat aagctgcaga
     7381 ccagccttca tgtaaatgct ttgcagttga gcttcgtttt caagacccgt caacgtactg
     7441 tggctcaaca ggtctctgac cgctgcatac ccttctttag cgccgacata atgtggttgg
     7501 aaatcggcat tggcgacgat gaagttcatg cggtaaatat tgtagccagt caacgagctg
     7561 ccgatgacgg gctgcccaaa aacgagtgtg ttccgaatga cccacgggga aaagacaaga
     7621 aaaagggaca ccaaaaacaa caggattccg ccaatctgct ttttggaaaa cggtctggtt
     7681 tcgaaccatt tttttgccag cataaccgcc cctaaaccga ttccaacccc cagcaaaacg
     7741 gtccgcgaga gggcggccag cccaaagaac agacctgaga tcacccaatc tttggctttc
     7801 gatgcatgat atccccttaa gaagaaatac attccgaagg aaaaaaagaa cgcggcggca
     7861 agatcacctg agccattatc cacctgatta atgacgggga cagagaaggt ccacatgagg
     7921 gtgccgagca gggcggtttt cacattgcgg ctggattctt tcagggttaa gtagatcgca
     7981 ggaatgcttc ccagatagta cagactttgc atgatcagtg ctgaaagttt ggtgggatgg
     8041 aaaagcctgg ccaatgcgat caacagcacg ggaatgggct cacgcatggc cgtggcctgg
     8101 ctggtggatt cgcaattggg aaagtaatcg gtggcgcatc cgctgtagcc ttttccgccg
     8161 accagattcg atgcgatggc ccaggcaccg ccgtcacttt tgaagatcaa gttgccgtta
     8221 aagtatgcat gaaagattgg agacaggcac agcatggcaa tgagaatgac tcctgccagc
     8281 caatactttt ttgtgtaatc ccatgaaaag atgggtctga agacatcaag catttgaaat
     8341 actcccctgt gcggtttgat atcttcgatt tgatgcgtat gcgatttcat gcctgaagga
     8401 tacccgttgg agatgacagt aaaatgacga ataattccgt cccgtcgtct ctttatgttt
     8461 tcaggtcgtg gagattgcat tgaggggcct ttacctgaag aaatgccccg gggagcaagc
     8521 gggaaaatca gatcggtatc ccgtctacga tctcctggcg gtgtttgcgg tagtcgcgga
     8581 tgagcgagtc gacacagcgt gccactgtgg cagggccgcc gcgataatgg atgatgcctg
     8641 tatccgcttc ccattcactt tcagggatgg tcttcaaata ggcggtcagt atttcctttg
     8701 tggcggcgat atctcttaac agtgtatcac ggtcggtggc tgaaaactgg ctaagaaatt
     8761 tcgcgttgac cttgcggtag tcgttcgtgc catcgtaaaa ataaaatggt tcggcacctt
     8821 cacgcagggc gctgcacccc gccagtgtga tgcggttcca gccaatgaaa tgcgcggcga
     8881 tgtcgcgcgg ggtccaatcg ccgagggtgg tgaaaaatgc ttcggatgcc agaccagtta
     8941 ccgtgtcgat gaaagaggcg taggcggttt caaggtccgc gagttgtttt tccttgtgat
     9001 caaacatggc ggctcctgtt tatttcaaac ttttatatcc agtaaaaccc atgccaagca
     9061 gggcaattgc gctggcaaaa ataaaaaacg gcacgggcaa aaatcctgat acttctttga
     9121 ggctggtgcc gttgagcgcc caggatgcga cgaagcagca gagaccaata tgtaggactc
     9181 ccgccaaaat ggagaggacc gacccgatca agcggatcat tcgtacgggc aggctggtca
     9241 gccctgcgag gagcagaatc agcgccccat acggaatgaa caccgcgcca aagaccccgc
     9301 gaaaggcaca gccaaggtcg gcataacttt tacagtcctg cccatcggcg accagttgaa
     9361 ttccaaaacc gatgaccgcg attccgcaca gtgctgtgat cgcggcagag acccaataga
     9421 cggggtgcgg tttgttcatc tggccttgag tgcggcatcc acttcgttga gatgttcgtt
     9481 gcgatgcaaa gcacggacaa cccagcgttt gttgtaattg tagatttctt caagcaaatc
     9541 ctgcggaaat acttcgagcc gagcatcgag atcggcggag gattcaatgg cgatccgtgc
     9601 ggcttcacgc gggggaatgg ccgcccacaa cggcagggac aggtcattga cgatgatgtc
     9661 aatctcaggg acaaataatt tgccgttctt cacggtcatg tccagcacat acattacacg
     9721 gcgatcccac caggcgaggt gggcgagggc aatggccact gtccaatgct cgccgacgcg
     9781 tgtttgcatt tcttcatcgt taaggctggc tgccagctgt ttgatgcgtt ctgttgaggc
     9841 acggttaagt tgtgtgtaat tggggtcaag tgacatgggg attctcctat tcttgaaatt
     9901 gttttacctg taatgaacca ctcttcaaca attccaacat cctgcgcgtg gactcggcta
     9961 cttcgtccac ggctttttga aatgccgctt cgtttgcctt ggatggtttg cgatatccgc
    10021 tgacctttcg cacgtattgc agtgcggctt cctgaatttc ctgttcggta acagggtggt
    10081 ccatatttcg caggggttta atgcttcggc acatgataag tctcctgttg ataaagtgtt
    10141 actgtactat ccatttgttt aataaagttc tcagcttcaa cgatttttgt ataacttctt
    10201 cgctttcaat atttccaaca gaaatgatgg atggaagttc cctgtatagt tcggggaggc
    10261 ccagtttctg ttccacttgt tcgcggatcc acttgttgta ggcgattggg taggtctttc
    10321 tgctaataaa aagtgcctgt aaaaattctt gaaaggcttt gtataaacgg tcaaatgctt
    10381 gaaagtataa tccgcggtga aaaaagaaag ggacatgatc cagatcatac gtacaagcgt
    10441 gacaggtcat tgccagtcgt tgggcttgca atggagggtc ataatatgga agccaggcgg
    10501 attgaagttg actgtaataa gagccgatct cgtgcatggg agccccataa gccagctgat
    10561 ttccaacttc caattcgaag ccgtccgggc cgccgccatc atcccaaatg gatggagtgt
    10621 actggcctgt aatcagatca aggtgcaact gcatgaaggg atgtgaggtt ttgaattgct
    10681 gaaacacagg gttggattgc aaggcagcct gccactttgt ttccaattct tgcatatctg
    10741 agtgggcggt ttcaggtttc acaaggaccg ccatgtcaag gtcgctttca ggtgtggcct
    10801 gtccacgggc gcaggaattg accaccaaaa ccgtatccac cgttgattgg ttgagaaaga
    10861 aatcgcgcgc gaactccgcc gttttttgat gcagcagggt tgggaatttc ggctgtggat
    10921 tcatttatgc ctttggccaa tccaacaaca ggatttctgc cagtaaacgt gaattgacgt
    10981 tgcgttccat tttctccagt gcagattcca ggtcactgac cacgcggcgg gcggtgggga
    11041 ggtcgaggcg tcccgcgagg aattcgatct ccatattgcg gtccacgttg atgagcgggg
    11101 tttccgcacc agccacacgc agcatcacat cacgccagta tgaaagccag atcaaaaagg
    11161 tctggcgcat ggtgtcttta tctttggcga gtttttcggc gtaggagaat ttttccacac
    11221 gcggggcggg gataagggtt tgcaggtcgt tcaggtattc ttcgcgtttt tcgagcaggg
    11281 tcacatcgtc tacgagtttg cgggcgtagc cggggcggcc tccgctgatg tgggcaagca
    11341 ggcgtgcgcg ttcctcatcc acaccttgat cgagcagata ggattcgatg gctttgacgg
    11401 gcagcggccg cattctcaaa atctcacagc gcgaaacaat ggtgggcaat aattgttcgg
    11461 gcgtgtcggc ggtcaacagc aaaagggcgt gggcgggggc ttcttccagc gtttttaata
    11521 acgcgttggc ggcactgtca ttggcttcct gaaaccgcag gaacagagcc acacgatatt
    11581 ttgcctgata cggtttaagc gaaagtgcgc gctgcacttc acgcacctga tccactttga
    11641 gtgtgccgcc ttcggcatcg gcctgcacca cgttcatatc gggatgttgc atggcttcga
    11701 tctgcctgca atcacggcac gttccacagg gcacaccggg ggcaatcggg tgcggacagt
    11761 tcaatgcctg ggcgaggtgc aaagccaatg tgcggcgccc cagtccggga ggtccgcaga
    11821 aaagatacgc gtggcgtgct tcgccgcgtg ccgcgtgctg gcggagcata tccaccgccc
    11881 attcatgtcc gagcatgttc cagttatcga tcatttgggt tacagggttt ttggttacag
    11941 gttgaaagtt gcaggttgaa ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnagggatta ttcctgcccg gcgcgcaggc gcaagtacga atcgtatcgt tcgggatgaa
    12121 ttgtacctgc tttcaatgcg gcgaggacgg cacagcccgg ttcatgtttg tgcgtgcaat
    12181 cgctgaattg acaatgcggc acaaggtcgc gcaattcggg aaaatacgca tccatttctt
    12241 cggcttcggt atcccacaat gccaggcttt tccagcctgg tgtatcggct acgtatccgc
    12301 cgttgttgag tgcgaacaat tgacgtgtga cggtggtatg gcggcctttg tttacggctg
    12361 tgctgatctc gttgacggcc aatcccagcc catcttgaat tgcattcaac aggctggatt
    12421 tccccacgcc gctcggcccc gccagcgcat tgatcttgtt ttgtatttgc gccttcaact
    12481 catcgagccc tgcacctgtt ttggtggaag tgtagatcac gcgatagccg atcgattcat
    12541 acaagccgaa ggtctcatgc gggtcttcca caaggtcaat cttatttgcc acgatcagca
    12601 cagggattct ttgcttttca gccacgacca gaaaacgatc gagcattctt aattttggat
    12661 ttggattcgc acacgcgaaa acgaaaacgg cctgatcggc atttgccagc aaaacctgct
    12721 gatactcacc ctgaggccgc ggatccaacc tgacgatggc ctgcttgcgc tcctcgacct
    12781 tttccaccac tccggaacca tctgccaaaa ccgtaatgag cacatgatcg cccagcgccg
    12841 caatatcgcc aacggcacgc ccctgtttga gttttccgcg cagctgacaa atgacaagac
    12901 cctccccggt atccacgctg aagaaaccag attgggcctt aatgatcaaa ccttgttttt
    12961 gaatttcgtt ggtcaaatgt ttttccttgg gtaaataggt ctgacagatc ttcgaagacc
    13021 tgtcaggcct ttgcttgtaa gtctatggta acccgttgcc gccgccgaat ggggtggggg
    13081 tatagggtgg ttcaccgatg gggccgaaat cgtagaagat gcgctgtgga gagccgtagt
    13141 aatacgtctc aaccatggcg cggaagatgg gcactgcata ctcggaccct tcgccgatat
    13201 tttccacgat cactgcaatg gcaatatcgg ggaggttggt attggcttcg ttgagcgtgt
    13261 agccagcaaa ccatgcatga gatttcccat tgcctgattc ggcggtgccg gtcttgcctg
    13321 ccacgtcaaa ttgcaggcca cgcagattga agcgggccgt gccgcgcgga ttttgggtca
    13381 ccatcaacat ggcctcgcgc agaatgtcga ggttttccga gcgcagaggg agcgtgccat
    13441 tggcttcagg gcggaaaacg agtttggggt cgccgtctat gggctgaatc ctctcaacga
    13501 tctggggcct gtacaaggtc ccgccgtttg cgatggcagc gacaaatctg gcgacttgca
    13561 agggtgtcac ttgcatatcg ccctgcccga tggcttggtt cacagcatcc acttctgttt
    13621 gcggatcagt gatttggccg ccttcttctt ccagctgtgc aatgccagtg ggagagccaa
    13681 gcccgaaggc acgtgccatg ttggcaatat cggtgccacg gctatagttt gtgaataggt
    13741 cccagccgat ctcccagaag tagggattac aggagcgcat cagtccttct tgcagggtga
    13801 gcagacccga gggggtgctg tctgaagaat cgcagaagtc acctctggca atgcgatcct
    13861 ggcaatgttg ccaggtccag tcgtgcatga cctgatcgag gcgggtgaaa tcgtattggc
    13921 aatcgtaagg cgtgtctttg atccaaagtc cgctttcgag cgcggcagac atggtgatga
    13981 tcttgaagac cgagccaagc gggtactgtc cctgtgtggc gcggttgagc aaagcacctt
    14041 cgggcagaag gtaggtgccg cccgcattcg ggttgttgcc ttcaaaatag ttgggatcaa
    14101 aatcaggcga ggaggcgatc gcaagaatcc gcccggtgtc cacttccatc accaccaccg
    14161 caccgcggaa gaattcgatg gagcgctgcg cgtacttttg catgttgcta tcgagggtca
    14221 ggtagatgga atcggcgggt tcggcagcgg tttggccaag ggtggagatg atggctccgt
    14281 tggggccgac aattcgcaaa atgccgccgt gttgccctgc caaatagtcc tcggcccatt
    14341 tttcaatgcc ggactggcca acgctttcac tgccgttgta accgaggcgg cgataggaat
    14401 tcaattgctc aggggaaatc agttgggtgt acccaaccga ctggggcgca atacctgtgc
    14461 cgaagtgata ccgcgagttg tattcatcag agatgaccaa tccgcccggg ctgatggcaa
    14521 gcaggcgctg ggcttcttcg cgggtgcctt cgcacatggg caggtaattg ccggggccgc
    14581 tggcttcgat ctggtctttg atgtattcag ggtcgtagcc gcataacttg ccaagctcgg
    14641 cggtcagggc tccctgcatt tcaaggtcga tctgatcggt ttggatgccg aatgcgaagg
    14701 catcggcttg ggaaacgatg ggcaggccat cgcggtcata gatatcgccg cgtgcaggga
    14761 cgctgtattc cattttgagt acattgcctc ccgccaattc aggcatgatg aggctgtcgt
    14821 cccaaacgac cttccaggca ttttcttcat tggcaagacg catgacgata ttccgttgga
    14881 tatcgcctgc cagcacggtt ttgtaggtga cgctgtatcc cacttcggcc ttgtatgggc
    14941 tgatgaccga cgagttgatc gcaaattcga tgctggccgc gctcatttca ttcaaagcgg
    15001 tgttccaacg ctgtgaaaaa tcttccaggg tcagtgcgtc gcggctttgc tgggcgagca
    15061 tggcatacat ggtttcaaaa tcatcttttt gcaaagcctg caaaaatgac gtgactgccg
    15121 cttgcacatc cggtgcggga ataatggtgg ggtttgcggt tggcaggatg ggagtggggg
    15181 tgacaaaaaa atcggtgatc gggttgcctt ctgctccacc tgaactgcaa gacgaaagca
    15241 gggccagtgc cacgatcaaa tttatccatc gaaaaacttt cattcgtttc ctctcaatca
    15301 accattcgat acgccgctca ggatcgcgcc tgaaacagga catgcatagt tcaataattt
    15361 ttgacaggcc atcggtactt ctgccgaggg agatatgccg aactgttgca gcatccgcac
    15421 gacgtggcac atgggcagcc ccatgacact ggcataacat ccgcgcatgg attcgacagg
    15481 ctggaaatcg gggtgctgaa tggcatacgc gccggccttg tcgagcgggt cgcctgtgcg
    15541 aatgtaggcg gcgatttcct cgtctgaata attacgcatc ggcacatctg tgatgcacaa
    15601 ttccgtcagt gttttgctgt cactgacgcg gtggaccgcg acgcctgtat aaacctgatg
    15661 ggttttgccg cgcaattgtt tgagcatgcg ttcggcttcg gtttcgtctt ttggcttgcc
    15721 aagaatctcg tcgccgtcga tcacggatgt atctgacccg atgatgatgg cttcaaagtt
    15781 cgacctgcct gcaacagcca atgctttggc ttgagcgagg cgcaatacat attcttttgg
    15841 tgattcgcct gtcagttggg tctcatccac gtctgagacg atgacatcaa acttccagtt
    15901 cccaagtgca agcagttgtc tgcggcgcgg agagttggat gccagcacaa gtgtggctac
    15961 atttatcatg cgcaagattc taacacagac ttttttacga ctttatggag gccagtccca
    16021 tggattcacc ggcgaaaaac gaccgcggcc agtgttttac gtctgattct ttgcagtaga
    16081 tgtaaaaaaa taggcatggt cttttctgct ttcatcgcgt gtctgccgaa ttcgctgtta
    16141 cccactggca ggttattcct tcacgttgta caatgtgaga aaatctgtct agtcaatgtg
    16201 gaggcaacat gcagttactg aaagatcgaa tcttgcggga tgggaaagta ttggggaacg
    16261 gaattttgaa agtggatggt tttgtcaatc atcaggtgga tccgcttttg atggatgcct
    16321 gcgggcggga atttgcaaaa cgatttgtaa atgttggtgc aaccaagatc ctcacggctg
    16381 aaatttcagg tattgcgcct gcggtcacca ctgctgttca tatggggctg cccgtggtgt
    16441 acgcacgcaa gaacaagccc attaccatgc cagatcaggt gtatctcacc cttgcgcctt
    16501 cgcacacgaa gggacgcatg gtcgagttga tcgtgtctcc tgagtatctc gaaaataacg
    16561 aaaaggtctt gatcattgat gactttctcg cttcgggtgc aaccatcctt gggttggtgc
    16621 ggttggctga agccgctggc tcacagatcg tgggcatcgg cgcgctgatc gagaaatcct
    16681 tcgaaggcgg ccgcgatgcg ctcgcgtcac tgggtgtgca ggttgaatcg ctggccaata
    16741 tcaaatcact cgataacaac aaagtcgagt ttgtggatta atcacaaatt acatttcacg
    16801 cgtgacccat tgcggaaact tcttcggctt cggcttgttt cccgttttat cggctggatt
    16861 ttcacctaca tgagttttct catcccagtc aaccgcctcc gtgatacccc aagcctgatt
    16921 gcgtttcatc atccttctcc tggctataag tttcacgtgc tcattgtccc gaagcggcag
    16981 gtggcctctc ttgccagtct tgatccatcg gatgccgttt ttctaaccga cctgtattcc
    17041 acggttcaaa gtttggtgaa cgagtttcag ctcaaagcct accgtctcat tgtgaacggt
    17101 ggggaatatc aggattttcc acatttacat tttcatctta tttcagatac gtagggacac
    17161 ggcaccgctg tgtctttaca gggattaatt atgaactcta ttgccattct tgattttggt
    17221 tctcaatatg cacagatcat cgcacgccgc atccgcgaag tgaatgtgta ttgcgagttg
    17281 tttccgtggg atgcacccca ggaaaaaata ctctccatca atcccaaggg attcatcctc
    17341 tcaggcggac cgaaatctgt ttatgaagag aatgcacctt acatccagaa attcattctt
    17401 gaatcaggcc tgccgatctt tggtatttgt tacggcatgc aagccctgac ccatgcactg
    17461 ggcggggagg tcaacccttc aagcgagcgc gaatttgggt ttgccgagat cgagcctttg
    17521 atccccaata cgatgcttga tgtgatctcc aaagtgtgga tgtcacacgg cgataaggtg
    17581 acacgcctgc cgcaggggtt catgccgctc gccaaaagcg gaaacagtcc atatgcggcc
    17641 atcggtgatt tgcaaagaaa atatttcggg gtgcaatttc accccgaagt gaatcacacc
    17701 ccgaacggta ccgaattgct caaacacttt gccattgaga tctgcggcgc aaaacccgaa
    17761 tggacgcccg cttccatcat tgacgaaagc gtgaagcgga tccgtgaaca ggtggggagt
    17821 cagcgtgttc tggcggcggt ttcgggcggc gtggattcgt cggtggcggc ggcattggtt
    17881 cataaagcca ttggcgacca gttggtgtgt gtgttcgtgg atacgggcct gctcaggcac
    17941 aatgaaggtg aacaggtcgc ctctgcgttt cggggcgggt tgggcgcgga attaatcact
    18001 gtggaagcca gcgcagattt tcttggtgcc cttgccgggg taactgaccc cgaacaaaag
    18061 aggaagatcg tgggggagaa attcatccgc atttttgaac agcaggcgaa aaacgtcggc
    18121 cagccgaaat ttttggtgca ggggacgatc tatcctgatg tggtggagtc gtctgcgcct
    18181 gaccgcaaca aagccgataa gatcaagacc catcacaatg tcggcgggct gccgaaggat
    18241 atgcaatttg aacttgttga gccgttgcgc tatttattca aggacgaggt ccgtttggtg
    18301 ggggaggcgc tcgggctgag tgaagctctc gtttggcggc aaccgtttcc aggtccaggc
    18361 ttgaccgtgc gctgcctcgg ggagtgtacg ccggagcgtg tatcccgttt gcgggcggcg
    18421 gatgcgatcc ttttggagga attatccaaa gcaggctttt taggaaaagg cgcaaagacc
    18481 tcacaggctt ttatggtgtt actgcccgtg aagtcggtgg gggtgatggg cgattaccgc
    18541 acctatcagg aggcggctgc catccgtgcg gtggtgaccg atgactttat gaccgccgat
    18601 tgggcgcgcc tgccgtacga tttgctggcg aaggtttcaa gccgtgttgt caatgaagtg
    18661 gatggaatca atcgtgtggt gtatgatgtg accagcaaac cgcctgcgac gattgaatgg
    18721 gaatagcatt tgtaacgcgg actttagtcc gcaccggctt tgagaaaatg attctacgat
    18781 tgatgaggga ctgccatgaa ctttgattac ggtgatactc tgaaacgagc cgcacagatg
    18841 accttgcggt ataaatcctt ttgggtgctg atgacattac aaatgctgcc atccatgatt
    18901 atgtttcctc ttttctttta cgcgctttgg gttttggaga acagccgcgg cgaagctggg
    18961 ggtgtctgga tggtcgtttt gggggctgct ttgatcctgc ttttttttgc cagttttctt
    19021 ctcatggcat attcccattc ggcggtgact ttaggagtga tccgtgtgga tcgcggcggc
    19081 gagattaccg atctgatgga attgctgcgt gatgcgttcc cattttatgg gcgcagtttg
    19141 ggtttgatcc tgtccattca gttgacgatt gggatgatct tcacggtcgt ttttatgatc
    19201 atcgggttgc tgatggtcgt gaccatgggc attggtgcca tctgtttgca gcccgtcatc
    19261 ctgctgttga ctcccttgtc catgctgttg atggctttca tggaagccgc gcagacagcc
    19321 ttgatcgcgg aagacctcgg cgtgctggat tctttgaaac gcgccctgca ggtggtccgc
    19381 gcccatgtgt ggaagtatgt cattatttcg ctggtcgtgt attttggcac atccattatt
    19441 tccggtatca tctccatccc gttgaccttt cctgccgcca tcctcgccat gtccactgaa
    19501 acgatgtccc tgtctgattc tcaaatgggg atggctgtca tggcttccat gttttgttgt
    19561 tttgtccccg ttatctttgc catttccggt gtgacccaaa cctttatcaa gacgaccctc
    19621 ggtctttcct acctgcggct ggccagcccg gcgggggagc aggtcatcac ccttgaagaa
    19681 caagcctcct aaaaactgca atatccaacc acagaacgca cagagatcac aggtagccct
    19741 gtggttctgt gccttttgtt ataagaggct tttctgcctg attggcattc ccccgtttgg
    19801 gctacttcgt aaactgcaac ctcaaattgt tttggacgta agaatccatg tttgatgttg
    19861 aaaaatcggt cgatttggcc ctaaagttac aaatccaaaa atccctgcgt ggacggattt
    19921 aatcgggtgg tgtatgatac tggtcagttc ctgacctttg taattttggg cacgaattta
    19981 gagttgattt tcagcacact tttgcgaaaa atctgaaaaa atgagatgag gagtccctat
    20041 gaatttcaac tttggagaag ttttcacccg tgcctggcag atcatttgga agcacaaggt
    20101 attgtggatc tttggtattc tagcaagctg tgcccgagga agtggcggcg gtggagggaa
    20161 ctccggttcg cggtaccaaa cttccggagg cgatgctccc ttttcaggtg gacaaattga
    20221 gcagaccttt aatcaaatcg ggcaatacct tcaggattat ctctggatca tcgttgttgg
    20281 tatcattgcc atccttttga ttgggctcat ttttatggcc cttggcattg ttggcaaagt
    20341 cggcctgatc aaaggcactt ataaagccga tgcagatgtt gaacgcctgc aatttgcaga
    20401 actgtggtct gaaagcctgc cttacttctg gcgcatcttt ggcctgaact tccttttggg
    20461 gctggcggtt tttgtgatca ttattccctt tgttttgctg agtgtgttca caggtgggat
    20521 tggcgcgtta tgtcttttgc cgctcatctg tattttgatc ccggtggggt ttgtagtctc
    20581 gatcatcacg gagcaggccc aaactgccat ggtgcttgaa gacctcggta tcatggacgg
    20641 tctcaaacgt ggatgggaag ttgtaaaagc caatattgtc acaatgatcg ttatggtttt
    20701 gatccttggc attggtggtg caattgtcgg catcgtcatc gctttgccgc tgatttttgc
    20761 cgcgcttccg attgtgattg gaatgggttc tctgggtgag tcgttgacac ctttgtacat
    20821 tgcactggct tgctgcgttg tctatttacc ggtgctggta tttttcaacg gcgtcatcac
    20881 cgcctacacc caatctgtgt ggaccttgac ctttatgcgt ctgaccaccc ccaaactcaa
    20941 agaggaaacc cctgttttcg ttgaggataa tgcgtaagtt ctttgcttta ctgttcagtt
    21001 tggggctttt gtttggggcc gcttcgaatg tgcgtgccca gcaaaacctt gcatccgcag
    21061 agctttaccc tgcggatgct tccactttcc cgactgtttc cgccttttta gatgttttcg
    21121 acacgaacgg cattttcgta tccggactca agcccgaagc tgttaccgtc atggaagatg
    21181 gacagcagct tccagtggat gccctgacgg agatcgccgt tcctctgcaa ttgaccgttg
    21241 ctgttaatca aggtccgcct ctcgatgctc gtgactcgac cacgaatatt tcccgctatc
    21301 agcgcatttc agaggtgctg gttcaatggg cccagagccg ccccgccgac ctgcccgatg
    21361 attacagcct tgtcagtcag gcggggccaa tcattaacca tgccagtgcc gccgattttg
    21421 tcgtggggct gagcacgttc cagcctgatt tccgagccgc tactcccaac ctgcaatcgc
    21481 ttgccatcgc gttggacacg gtcagcgcac aaaccacccg gttgggtatg aaacgcgcga
    21541 tcctgttcat cactccgcac accgacgaca tcaatatcgc ctcggcgctt gagccatatg
    21601 tgcagcgagc catcgaaaac aagattcgaa tcttcgtctg gttcgtggac ctggaaacga
    21661 cattcaccac caccagtgcg gcggcattca acaaccttgc catccagaca ggcgggagca
    21721 tgttcaaatt ctccgggctt gaaagattcc cagacccgga agcgtatttc tcgcctttgc
    21781 gccgtgtgta cgcactttca tacacctcac gtttgaaatc ggtgggggag cactcgttga
    21841 gtgccaaagt gattttgccc ggcggacagt tgacctctgc cgagcagaga tttgtcctcg
    21901 atattcagcc gccgaatcca tttcctgtta cgaactcgct gcagatcaca aggcgcgccc
    21961 ctgaagatga cccattcaat acggaagtgc ttttaccatt tgcgcaggaa gtggagatca
    22021 ttgtcgaatt ccccgacggg catccccgcg ctttgacccg caccaccctg tacgtggatg
    22081 gcgtggtcat ggatgaaaat accgtcgagc cgttcaatct attcacgtgg aatctggcgg
    22141 cttacaccac caccggtgaa catcagatca tggttgaggc ggtggatgtg ttgggattga
    22201 gcaaggccag catgcccgtc ccagtggtgg tgacggtggt acagcccccg aagggaattt
    22261 ctgccttgct ggcgaagtat cgcaccccca tcacctttgg cgcgatcctg ttggcggggc
    22321 ttgtgctatt tgccatcctc atcagcggaa gattccgcct gccatccatc cgcgctgcac
    22381 aggaagcacg ccgcgctgaa attgacccgc tcacacagcc gattcgtgcc ctgggtccgc
    22441 tgcccacgct ggcagaagag aaaaacaaaa agcgccgaag cattccaaag aaagccgcaa
    22501 cagatcccag gtcaagtgca gaggcgatgg cttccttcat ccgagtcaat gccgatggtg
    22561 tgcctgctgt ggcggcgccg atccaactct ttgaaaagga aatcaccttt ggtaccgatc
    22621 ctgtgcaatg cactcagatt ttagatgacc cctccattgc gcccatgcat gcccgcctca
    22681 gacagaccga agacggagga ttcctgcttt tggataacgg ctcgatcgcc ggtacctggg
    22741 tgaattatga acccatcccc cgcgagggcc atcggctgat gcacggggat atggtaaact
    22801 ttggacaatt aagttatcgg ttcacgcttc gcataccacc ggtaaactcc aaacccaaaa
    22861 ttacggtcca cagcatcgaa gaatgattcg ttcttccctt cctcacctac acattgcagc
    22921 acttagtcat gccggtatgt ctggcaagaa taatgaagac cgttatgcgg tttcatcatt
    22981 ccagctaagc aaggatgatt cgcgcccggc gctctttgcc gtggtggcag atggcattgg
    23041 cgggcatcgc gccggggaag tagccgccga actggcggtt aactacatca ccgcagtcgt
    23101 tgccgaaagc aatgccaaaa aacccatcag cattctcgaa aatgccattc accacgccag
    23161 ccaatccatt gccgcgcact cagcaggcaa agaggatgaa gacggcatgg gctccacctg
    23221 tgcctgtgtc tgggtgatcg agaatcgcct gtacactgcc tatgtcggtg attcgcgtat
    23281 ttatctgttg cgcggcaagc acattcagcg cctcaccatt gaccacacct gggtgcagga
    23341 agcctacgaa aaagggatca tcactgccga gcaaatgcac gaccatccca acctgcatgt
    23401 catccgcagg catttgggcg gcatcaaact gcccgaggtg gatttcaggt tgcgtatcga
    23461 taacggtgaa acgaacgagg aatccatcaa caatcagggt ttccaccttg cccccggcga
    23521 caccatcctc atttgcagcg atggcctaac cgatctggtg tgggacgatg agatcctgaa
    23581 aaccattcgc tcaaagaaag atatgaaggc ctccgctgaa gcgctcatca atttagccaa
    23641 cgagcgcggc ggccacgata acatcaccgt tgtcatgatg accatgccct atctcgaaga
    23701 aacgggcaaa aagaaatcag gcatcctcga ttggctgctt ggcgaagatt agcaaaacgg
    23761 ttcatgaaga aaaacttcat gaaccgtttt tttattttta atggtttttt gggggaattt
    23821 gcctgcggcc gcttcaaacc ccgccgaaac ctgattcaac aacgatgaac agaatctcag
    23881 cccggagagg tttatcaggg ctttttgatg ccgcgttcac ggtcgagcca taactcgcgg
    23941 tattttctgt tccaatcgat caaaaatctg accgcgatta ccgcacccat acccagggcg
    24001 gcccacatcg aaatatcgcg gatgtttaac agccacaacg caggcgcagc cagaatacct
    24061 gcaaacacac tcgcccgcgc tgagtggcgg ataagcaaca ccagcagaag caacaaccca
    24121 aaacagacga ggaacgccaa tgggttgatc gccagcaaaa cgccgccagt ggttgccaac
    24181 cccataccgc cgcggaattg ggcaaagacg ggccagcagt gaccgatcac cgcaaatgaa
    24241 gccgtcagca caaggataag cggagaagca cccagttgaa tggccagata ggttgggaga
    24301 aacccctttg ctacatcaag cgcaaagacc agcgcgcccc agccaaaacc cgcctgacgg
    24361 atggtgttgg tggtggtggc atgccccgag cctgcatcac gcacgtccac ttttttgacg
    24421 aagcgggtga cccaaatgga aaacgggagt gatccgcaca ggtagctgaa aatggggagg
    24481 agaaaggttg gcatgtgggc tacgggcaaa caggcaggtc ggggaagaat tcctctccgt
    24541 atttcaggag caggtcgctt tcttcgatgg ggaaggtgag cagcaccgat aacaagcgcg
    24601 atttgttgag cggttcggcc cataagcgga tcttgtattg ctggccttct tccacggcat
    24661 ccacttcata gagcaggagt ctatccttcc cgcaagagga gacaagcgtg gcttcgtcgt
    24721 agccttcgaa catgatcttg agggcgtctt cgctgtagaa ccaggtgaca tcgtcggtgc
    24781 cgacttcatc acagaacatg tacacggtga catccgccag cgccgacatg gtgcggtgtg
    24841 tccatgaaat ggtggattgc agctccttga cggtataact gcgctgccag ttcacctcgg
    24901 taaatagttc ttttccgaat tccaacccat atttgacgtc gttttctgtg ggctgcagag
    24961 aagcgcagct tacgctcaga tcagattttt ccacgctttg cgaaccgcag gcgctgatga
    25021 gcagggcaaa agcaaaggcg aaaataagga cataaatttt tttcattgat gtttctcctc
    25081 tttggcaggt gattccctca gtgcatggca ctggggggat gctgaaaatt aatggaacag
    25141 gcgcagtgtt gtgctttttc ctggctttgt cagcgaggca ccttgaatgg tgtgatgtct
    25201 gggtcaatgg tttcgtcaaa ttcacgggcg gcgagatgtc cgctgaagat ggcttgggcg
    25261 atgatgttgg gtgcttcggc gtcgccaata cggcgcaggg ttttgatttt gccttcggct
    25321 agtgcaggtt gaagctcgtg gtagagtgtg tcgttgggga ttcggtctgt gaccatgacg
    25381 atggcatcgc agggcagggt cgtttcggcg gaggtgatga cgttcgtgac cgttgcagct
    25441 ttcgggtcgt aggaagcgat gacgtggttt ggaagcaggg taatgttcaa gtcatgcatc
    25501 cgcttgagga tgcgatcctg ttcgagggtg aattgagtcc agtatgaaat gctgggggcg
    25561 ggggttatca atgtgacttc gcagcccgct tcgggttggg cgagtaattc ggcgaggacg
    25621 ccgcccatgt agtagtggtc atcgtcgtag atgatgactt tgcctgacgg tagattgtga
    25681 ttcatcaagt cgtcgggggt gaagatcttt ccattgcctg tcacggggcg ggtgaggtag
    25741 cggcccatgc catcgcgccg ccaggttgcg cccgtggcta ggatgatgtg cggcgtgcca
    25801 gtttcaagaa tatcctgtgc ggtcatgggg ctggaagggt agaagaaaat attttcggtt
    25861 ttgttgatct ttgtgatgcg gtaatccacc acgcgccgcc attcgttgag gttgggcagt
    25921 gctgattcaa gcagtacacg cccgcctgct tcacgccgcg catcggtgag gatgacgtga
    25981 tagccgcgct gaccaagcgc gctggcggcc tcaagccctg ctggccccgc gccgacgatg
    26041 aggatttcat cttcgctttt ctttggcgca atgatctcag ggtgccagcc gcgccgccat
    26101 tcttcgccca tggttgggtt ttgtgtgcag cgaatgggaa cgaagcgtgt atcgccagtg
    26161 acgcagatgt tacagccgat gcactcacgg atgtcttcat ggcggttttc tttgatcttg
    26221 ttcggcaaaa atgggtctgc gatgctggga cgtgccgcgc cgatcaaatc catgatgccg
    26281 cgttcgatgg cggagaccat tgaatcgggg gaggtgtagc gccccacgcc gaccacgggt
    26341 ttggtggtga gctttttgac gaaggaaatg tatttttcct gatgaccttc ttttccaaaa
    26401 cgggaggtga ccgagtcgtt tgcccagttg ctgatgttca catcccacag gtcggggagt
    26461 tcggcgagca taccgacgat gtcgcgtgct tcgccattgt gctgcattcc gtccacaccc
    26521 agcaattcat ccactgcaaa gcggacggcg acggcgcagg tatcaccgac agcatccttt
    26581 gtatcttcaa tgagttcgcg taaaaaacgc acgcggtttt cgagcgaacc accgtattca
    26641 tcggaacggt cattgtcttt ggagagcagg tgataggcaa gggtcatgtt gtgcgcggcg
    26701 tagcaataga cgatgtcgaa gcccgccttt ttcgcgcgga tggcggcatt gcgatgccat
    26761 ttgcggactt gtttcaggtc ttcttttgag gcggcgcggc tttgaccggg ttcgtatccg
    26821 ctgccgccgg tgatgcctgc cgagcgcggc ccgaaggcag cggcccgtga atataggttg
    26881 gcagcatcgt ggctgttgta ggtgagttca atgcctgcca atgcgccgtg tttgtgaacg
    26941 gcttcggtca ttaattgcca ggcggggatg tcgtcatcgc tccagatgcg gccttcgaaa
    27001 aacggagaaa ggtcgctggt gtagtgaatt tcagtttctt cggtgcagac cacgccccag
    27061 ccgccttcgg ctttcattcc gcgcatggcg gccatgcctt cgggcatccg ccagccgaag
    27121 ccgttgcagt gcggcacttg atagaaacga ttcggcgcag tgacagggcc gatctgaatg
    27181 ggttggaaga ggatatcgta acgggaggtc atgtcttttt cctaggagca gtttggcagg
    27241 ttgggaaaga ggcggatgga gtattcgtcc agaagcggtt gtgattcggc tgggaatacg
    27301 atcatggtga cgatgatgcg gttatctgta tcgctttcga cccagtaatt gatcttgtat
    27361 tcgaagccga ggttgtaggt tttgaattgg tacaacctga tccctgaatc aagtttgcat
    27421 tcggcaatgg attcgtagct ttcatagttg gcgaagatgg cgctccaggt ggcttcgctg
    27481 aaatcgcggt tgagatcggg ctcttcgtag ccgcagggtt tgatccgcgc ttcgaggtag
    27541 gcaacggcac cctgcacaat gttttgccag gtgatggaat acgcgccttc cagtgcggct
    27601 tcgctttgat cccaacttga gtcaaatgca tcacctgtgt aggagtgtgc gcggtcaatg
    27661 tcttgttgag tgggctcagg cgagacgcag acaggcgtaa ctgttggcag cggtgtgttc
    27721 gtcgctacga ttctgggtgt ggatgtgatg gtgggtgggg ggagcggtgt ggaggtgggc
    27781 ggtggtgcag ggggctttcc acaggatgaa agaataactg cggcaataat gaggatgaag
    27841 atttgttttt tcatgtttac tcgtctgggt cggcgtttga tacagacgag tatagcacga
    27901 caaaataggc ggggcaattt atttgcccac cttgcacagt ggacttgata cgatgcgaaa
    27961 tttatccgca ctttgaccgc tgaccatgaa ccacagacgg ttgtctgtgg ttcagttctt
    28021 tgaagcggcg tatcaactgt gctgggtaaa gtgaggtgaa aatttacaaa cctttatggc
    28081 tttatccccg taaacatgcg cacccaaaaa tcggatttga aaatgagctg ctggttttcg
    28141 agttgggctt gatccagacg ggcatttatt ttgtccatgc ctgcctgata ggtttcttca
    28201 ctgagcaggg ccagttgtga tgtggcgtta tgtttgatga aggggtcatt taacacggcc
    28261 ttgcccacat gcaggttttg aatgtgctcc acgttttgca tttgaaatcc ccgaaagcca
    28321 gcctggtcca tcaggatttc aattgttctg gctgagggat aacggctgag gtcggtttca
    28381 tagacggtgt caaaatactc gtagatgtac cagctgccgt ctccgctgtg gggatcgtgg
    28441 ccgatgaccg ccagggtgcc gccaggcttt agaatgcgga aggcttcgtt gacaaaggct
    28501 gcatgatcgc caaagtgatg aatcgcatcc acgcagtaga caaggtcaaa ggtctcgttt
    28561 tgatagggga gttgggtggc tgtgccgcgc gccagttgga tgggagcagg ctgttggcgg
    28621 gcctgtgcga tcatccccgc tgaataatcc atgccgtaaa gctggggggt gacctgatga
    28681 agtagattta gccagaagcc tgtgccgctg ccagcctcca gaatgcgctg tgacttggtc
    28741 cgtttggcaa gctccagcag ggcttgtccg cgctcccaga actgtgtgga tgggtagcgt
    28801 tggttatatt cagatgcgat cttgtcgtaa ttgagttttt tggtttgcat gtttttttga
    28861 gcgccccgca ttgatttttc ttaccatgct acacaggcac gatctctttc acacgcccca
    28921 cctgtccatc catcaagcgg actttgatgc cgtgcgggtg gttgggcgat ttggtgagga
    28981 tgtctttgac gacgcccttg gtgagtttgc ctgtgcgttg atcctgtttc aggacgatca
    29041 gcacggtgat gcctggtttg atgttggcgc gggtttgacc gttcattgct catgctccat
    29101 gggggtcatc cacgggcgga cctcgaagat gagcagacca gatttcacag caggatcagc
    29161 ggcggcgaag gcttcgatct cttcctgtga aacctctttg gtcgtaacca tcattccgcc
    29221 cacatcgggg aggaggaacg ggccgcccaa ttgcaatttg ccttgttgat gaaactttag
    29281 ataatgttcc acatgctcgc caacgccatc ctgctggcga taatcgatgc cgtattgcca
    29341 tttggggcca ggcttatgga tgacgacgta acgaaccggg cgcgtgggtg ggtttgtcat
    29401 ttttt
//
LOCUS       DAOQJC010000046        29062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_692_length_29062_cov_8.553694, whole genome shotgun sequence.
ACCESSION   DAOQJC010000046 DAOQJC010000000
VERSION     DAOQJC010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 29062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29062
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_692_length_29062_cov_8.553694"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1284)
                     /gene="fahA"
                     /locus_tag="PLE39_08910"
     CDS             complement(37..1284)
                     /gene="fahA"
                     /locus_tag="PLE39_08910"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HNE68488.1"
                     /translation="MSKSFIPVSPESHFPIQNLPYGVFSTAEHQGPRVGVAIGEFVLD
                     LAVLEQKGYLKTKHIFNHPNLNSFMALGRSVWHETRATLQALLSDDNPILRDDPDLQK
                     QVLHPMQTVTMHLPAAIGDYTDFYSSKEHATNIGSMIRSKENPLMPNWVHIPIAYHGR
                     ASSVVVSGTDIRRPNGQTIAEGADGPTFGPSRNMDFELEMGFFVGPGNPLGHPIPIEN
                     AEEHIFGLVLINDWSARDIQKWEYQPLGPFLSKNLATSIAPWVVTLDALEPFRCENPS
                     QSPTPLPYLQSKGAASYDIKLEVSLQTKQMNSPVTIARSNFRYMYWTMRQQLAHHTVN
                     GCNMRPGDLLGSGTISGPTQDSYGSLMELTWRGEHPLNMPSGEKRTYLEDGDALTLTG
                     WCEGEGYRVGFGEVTGKLLPAHN"
     gene            complement(1331..2257)
                     /locus_tag="PLE39_08915"
     CDS             complement(1331..2257)
                     /locus_tag="PLE39_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68489.1"
                     /translation="MTLPVAKSLREFLKNLIDYAGLFPPASLDLASALDEYKAYLPST
                     DNWMLGRFIISAADLSKLPNLSDAWHFSVLGRSGKNADEFAQNLENDLNDIRSFRQHQ
                     PAFADVFEVRLPSEATPDLLKYANEKITSQNKLTAYYEIPITPAWRENMTATIIALSE
                     SNQTANLRNGFKLRCGGVVADAFPSVEQIAHALLLCRNHHVPFKATAGLHHPIRHYNE
                     SVQTKMHGFVNVFGAAILAQAHNLNTEQTVAILNEEDPQHFRFTESHFTWGNFSVTAD
                     QIADSRRSQFISYGSCSFDEPREDMQKLTWLS"
     gene            complement(2333..2779)
                     /locus_tag="PLE39_08920"
     CDS             complement(2333..2779)
                     /locus_tag="PLE39_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68490.1"
                     /translation="MKQSAFYFIVVSVLILFLAACTPPATATQPPAEASPVPSETPTA
                     IPSATATPSPLYIVINIDFAYLRSGPDVDYPTISDAYIRGTKMEVLSKYKDWFYVKAP
                     DEKIGWLYLEWIDASGIDLNIIPTPSFLPEPPVRPTPKPTNPGYPN"
     gene            complement(2864..3484)
                     /locus_tag="PLE39_08925"
     CDS             complement(2864..3484)
                     /locus_tag="PLE39_08925"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE68491.1"
                     /translation="MNRKLPAKFQNLLLRYSQEVNNVNREAIEAELWNEYGSERAIFV
                     LDMSGFSLLTRKYGIIHYLSMVRRMQLVSEPIITSFHGKVIKFEADNCFAYFPDTLPA
                     IQAAIALQHAFDASNLLTPDDFDVHISCGIDFGKILVIGNKDCFGDAVNRACKLGEDT
                     AAAGEILVTQKAMERVSPESGVKSHPLNVSASGITIPSHTIDYRGK"
     gene            complement(3565..4986)
                     /locus_tag="PLE39_08930"
     CDS             complement(3565..4986)
                     /locus_tag="PLE39_08930"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNE68492.1"
                     /translation="MDIITGKILKLNNWPDGRIIGLAKDAAAKLMEQGMERDAVLAQL
                     EAVRQNPGSFLADTVTVDLARECIRTMQKDEPKPETLREGPLPFPIWGRDNIDDEAVK
                     QMNNAMRLPVTVTGALMPDAHVGYGLPIGGVLATENVVIPYAVGVDIACRMRLSVYEV
                     SPNLLGQKKGLFEESLWNETAFGMGAKWTGAKKAQHEVLDDSAWFATRQLQTLKDTAM
                     NQLGTSGTGNHFVEWGSFKLHEDTFGLKAGQYLALLSHSGSRGVGAKIADRFSKLAME
                     KHPNLDKSVKHLAWLSLDSEEGQEYWLSMELAGRFASANHYIIHKRVAAAIGLKEVAA
                     VENHHNFAWHETLADGRKVIVHRKGATPAGKGVLGVIPGSMGDAGYVVRGRGVENSLS
                     SASHGAGRKMSRRVAIDSISKSARDVYLKERGVTLLGGGLDESPQAYKQIEDVIAAQN
                     DLVEIVGKFSPKIVRMADEPGDI"
     gene            complement(5028..5456)
                     /locus_tag="PLE39_08935"
     CDS             complement(5028..5456)
                     /locus_tag="PLE39_08935"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNE68493.1"
                     /translation="MTQPASTEMIHAARERFNSAIATKDADIITSLLAPNYHLITGRS
                     AQFHGAEEEGIRWSELFRTDPTAIYRRTPREVTTNESWGLAQEIGNWQGSYTAEGILV
                     HASGVYAAKWQRAKSGEWMLQAEVFTTLTCEGPCDPPDPI"
     gene            complement(5499..7295)
                     /locus_tag="PLE39_08940"
     CDS             complement(5499..7295)
                     /locus_tag="PLE39_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68494.1"
                     /translation="MTETTFTQKVPALPAWKVIFEMVRFRPWLWVVDLFSVALIRFCW
                     QVAPALIIKAFFDMVTGEAQLTFGIWAIVAFLAATWIGRVVASYGFYHADVPIFAEMN
                     TLLRKNLLRYILKRPGASQLPDSPGEAVSRFKKDVDEIPLFVILINDIMVGLIIIAVA
                     IILMTQISPSVTVIALIPLALVGIVANLATGRIEHYRRSSRQAGGKVTGFIGEFFGAV
                     QAVKVATAEKDVIHHFHKLNDDRRVLTIREKLFDEVLGSIYRNMSTLGTGVILILVGQ
                     SMREGNFTLGDFSLFVYLLQSMGDLTTFGGMLAARYKQLDVSVQRMYHLMENAPLNAL
                     VEHSKIDLDGPLPEVIYPTKTASDSLRSLSADHLTFHYPDSVNGIEDISLKINRGSLT
                     VITGRIGSGKTTLLRALLGLLPIESGEIRWNETILTAPGDFLIPPRCAYTAQVPRLFS
                     NTLRNNILLGMQRSDEEIYKATKLAVMDRDLEQLDDDLETMVGARGVKLSGGQAQRTA
                     AARMFIRESELVVFDDLSSALDVETESQLWERIFAAGNQLTCLVVSHRRPLLRRADHV
                     IVLKDGKVESEGTLDHLLTTSQEMRELWKLEE"
     gene            complement(7384..9129)
                     /locus_tag="PLE39_08945"
     CDS             complement(7384..9129)
                     /locus_tag="PLE39_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68495.1"
                     /translation="MNLSFKSYWNLLSDHIRPQKGRFILLAALLFGSIGLRIFAPQIM
                     RRFIDSALAGEALSTLMWTAIAFIGIALIQQAVAVSVTYLGENVAWTATNDLRAELAE
                     HALHLDMKFHNDHTPGELIERIDGDVTELATFFSQFALNLIANGLLLVGILVALFLED
                     WRAGLAFAVYSILTIFVLGRLKDIAVPHQKARREADAQIFGFIEEQLAGTEDIRSSGA
                     VDYSIRELFRLQGIKLTHDRKAHFKRWIIENAMGFALTTGNLLAISSGYWLFSAGLVT
                     IGTVYLFVHYINLLEEPFWAMTHEIESFQTIGACVERLTEFRNFKAEVVSGVGVEIPA
                     RPLELTFEDVTFSYNGEDTVINGLSFDLRAGSVLGLLGRTGSGKTTLGRLIFRLYDVN
                     AGSIKVNGADLRDAHIETLRRNIAIVTQDVQLFRASIRDNLTFFDRTISDEKIIATLE
                     ELELGDWYQNLPKGLDTELETGSRSLSAGEAQLLAFTRVFLRNPGLVILDEASSRLDP
                     ATEQKLERAIDKLLKNRTAIIIAHRLDTIERADNVMILDKGKVSEYGNRKELAADLNS
                     RFYQLLQTGMEEVLA"
     gene            9436..11124
                     /locus_tag="PLE39_08950"
     CDS             9436..11124
                     /locus_tag="PLE39_08950"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNE68496.1"
                     /translation="MKNTYRMRYWRIVLFFARVTLSIIFWDIFLPRIGLRFLSRNTRP
                     QRLRGIAVRFRALAIRLGGVMIKVGQFLSARLDVLPPEITDELAGLQDEVPPVDYEAI
                     RVQTELELGSALEKIFLTFEKAPIAAASLGQVHRARLFPHEAETIGFENIVVKVLRPN
                     IEQIVDVDMSALRVVGGWLKRYRPVSDRADVPAIVEEFAATIREEVDYLSEGKNAEAF
                     AINFKGDENVYVPRVVWTRSSRRVLALEDVSAIKITDYEAMTAAGISRAAVADRLLAT
                     YLKQIFEDGFFHADPHPGNLFVAPLHEKNADGSTKFRLTFIDFGMVGRMPERLVEGLR
                     EAVISVGLQDATRLIKAYQTLGVLLPSANVKLLEEASAQVFDRFWGMSMNELRSIRHD
                     EMMKFGLQFRELMLTMPFQLPENLLLLGRTIGILSGMCTGLNADFNLWLQLAPYAKKL
                     TEGENGSAFDVFLNEAGELLKTLIAIPGRAERVLGRIERGELNMQMPLVNIQISYLER
                     SLNRLTGGVVFLGLLVSGAILYDGHALLAQGMFGGAVLALFYTTFLARGKRPFQ"
     gene            complement(11186..11401)
                     /locus_tag="PLE39_08955"
     CDS             complement(11186..11401)
                     /locus_tag="PLE39_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68497.1"
                     /translation="MNEEKRKSMRDKLPPEALEHMKAARQEMRKSMETILPPGFLEHR
                     RAARKEALLAFRSLIDAAIKRTEEKSS"
     gene            11501..12304
                     /locus_tag="PLE39_08960"
     CDS             11501..12304
                     /locus_tag="PLE39_08960"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE68498.1"
                     /translation="MRNSFTLSIKLIFTLAAIFVLTACGVIAPASPTPDVLATVVAQT
                     LTSMPNPEVAASTSTPVAATFTPIPSTFTPIPVSVAATSTPPIGGANFFFTSAENVNL
                     RVNPGRLFQVSRVLAKGTRLQVLGMAPGGQWFNVVNDEGIIGWVGKDFLEGNPNQQNL
                     LVITPTDVLTITGQVLDVNGVPVSGIGFAAVQGDKRDDGVTDAAGMFYIFLPSKFTGA
                     WTTSFVSVACPSTTMDANCNCLNGTCGKPDPEVVTGMLPLSAPLSYVWK"
     gene            complement(12377..14083)
                     /locus_tag="PLE39_08965"
     CDS             complement(12377..14083)
                     /locus_tag="PLE39_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNE68499.1"
                     /translation="MSSEEVVSRIPAFIREAVAEDLKSGRFKHIRTRLPPEPNGYLHI
                     GHIKAFLIDYFTAKEFGGELYLRFDDTNPSKEETEFVNAIEDDAKWLGIEWAKVTFAS
                     DYFDLLYEWAVRLIKMDLAYVDDQTQEEMSAGRGTLPKGTKWSETESAIRPGVDSPYR
                     NRPIEESLDLLERMKNGEFPEGSRVLRAKIDMTHPNILLRDPVMYRIMHVHHHRTGDK
                     WHIYPMYDWSHGQNDSMEGVTHSLCSNEYIIHRPLYEWFLEKLEAFPSRQIEFSRLNL
                     TYAMMSKRKLRKLVEAGIVRGWDDPRLTTLRGLRRRGVTPEAIINFVTGIGETKNDSW
                     VEMAQFEAVIRDDLNKRAIRRMAVLNPIKLVIDNYPEGVSEELDAVNNPEDPNAGTRK
                     VKFSKTIYIEQDDFRETPPPKYYRLYPGNEVRLRYAYFVKCTHVVKNDQGEVVEVHAT
                     YDPATHGGDSPDGRKVKSTIHWVSADHAIPAQVRMYDQLFTVERPDDGELESIINPKS
                     LDVIENAFVEPALAEVKTGENIQFERVGYFCADPDSTPEKPVFNLTVNLKDTWAKLEK
                     KK"
     gene            14245..14580
                     /locus_tag="PLE39_08970"
     CDS             14245..14580
                     /locus_tag="PLE39_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68500.1"
                     /translation="MTESNQNRTNFAGTYFDRDAVLRLTRLLKIASWIVAVAYIFQFI
                     TNISVLILQISRDLIYIGGPTDLMLQIVWQIQPSLAGVIFYVALQAIAQTLLILLDIE
                     DNTRRAARK"
     gene            14701..14771
                     /locus_tag="PLE39_08975"
     tRNA            14701..14771
                     /locus_tag="PLE39_08975"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14733..14735,aa:Gln,seq:ctg)
     gene            14925..15395
                     /locus_tag="PLE39_08980"
     CDS             14925..15395
                     /locus_tag="PLE39_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE68501.1"
                     /translation="MIVKAVEADGEKIQDITARAGVFNEEEVDCVREIWDEYITLGPE
                     QCGYQFIVYRDGEQVLGFACHGPRDLTSGVFDLYWIAVDPSTRRNGVGRALLTASEDA
                     VRKAGGRMLIAETSGTPHYESTRNFYFGMGYKDEARIKDFYTVGDDLVIFIKRI"
     gene            complement(15493..16512)
                     /locus_tag="PLE39_08985"
     CDS             complement(15493..16512)
                     /locus_tag="PLE39_08985"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE68502.1"
                     /translation="MHNERPKILIIDDDAVARAAVEGLLASEPYETYFASNGFDGISM
                     AVNLHPDVVLLDVMMPKMNGFEVCRSIRSMPEIAEAPIILITSLDDHESRIQGLRAGA
                     DDFVTKPYNSMELFARLQTILRLNRYRRIAEQKEELQTLNKQLLVAYNKTIEGWSQAL
                     DLRDRETEGHTQRVMNLTIRFAKAVGFSEADLEHVRRGSLLHDVGKLGIPDAILHKAA
                     KLTDEEWFIMRKHPVYAYQWLSSIDFLEPALDIPYCHHEKWDGSGYPRGIKGEAIPIS
                     ARLFAIVDVWDALCSDRPYRSAMSKADALDYIRSESGTHFDPELVKIFTELVKDEIEG
                     NSQVP"
     gene            complement(16547..17194)
                     /locus_tag="PLE39_08990"
     CDS             complement(16547..17194)
                     /locus_tag="PLE39_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE68503.1"
                     /translation="MAHIRIVLADDHKLVRQGVRSLLEAQPGFEVIGEASDGQEAYKL
                     IESLSPDIAFLDVMMPNLNGIETAKLVHQRALKTRIIFLSMHANATYAVRALQSGALG
                     YVLKDSDFTEVLNAVEHVLEGRRYLSTAIADEVLEMLLSAEAGKDESLDLLSPREREI
                     LQLVAEGNSNATIAEKLSLSVRTVESHRLNLTKKLRITSHADLVKFAINQGLITS"
     gene            complement(17214..18290)
                     /locus_tag="PLE39_08995"
     CDS             complement(17214..18290)
                     /locus_tag="PLE39_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE68504.1"
                     /translation="MKKQPYTILVIDDEEIARTTLAALLEKSNFQVEMAEDGIQGLEL
                     AKRIHPDVILLDVMMPKMNGYEVCKYIRSDEKIAEVPIIMITALDDHEAKLNGLSVGA
                     DDFLSKPFDRLELDIRLQTLKRVNRYRHLMEEREKLQEALNELSKKHEQLRILSRQIL
                     EAQENERRHVAVELHDEVGQLVTGLKLILERKESDMSMVVAEARSVTADLFQRLREMS
                     LNLRPTTLDDFGLFAALDGLFKRFTKQTMITINHNINPLEERRFDKVIETTVFRVVQE
                     ALTNIARHAEVNTANITITVTTNHLQVSIADAGKGFDLKSKDQSASIGLSGMAERVNM
                     AGGFFTLQSTPGKGTFILAEFNLN"
     gene            complement(18287..>20578)
                     /locus_tag="PLE39_09000"
     CDS             complement(18287..>20578)
                     /locus_tag="PLE39_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE68505.1"
                     /translation="IRTCLLSAQYFPEKEYLEGSIMDITSRKQAEDWISRLNHQLESI
                     LNSAGEGIYGTDSQGRVTFINPTMARMIGWEVVDLLGRPIHPICHHTRPNGSPYPEVE
                     CPVYQAFHDGIARHSIVDHYWHKNGLPIPIEHTVTPIREGDKLIGTVVVVSDITKRLQ
                     TENETHQHMVEMEMLYESGLALSQLLSPEEIGQKIIELLERKMDWHHTAIRLYNPEND
                     TLKLLAFSQPNTTNKDEHVAVKHQFNNKITKSSDGVSGWAVKNTQVVRHGDISNDPRY
                     IETYHGINSGLYVPVRLGDNVLGVISIESEKPNAFSEKDERLVVTLANQAAVAFENAR
                     LFQTAQQEIIERKKVEQLLAEERNQLAQHVQERTADLSNANMDLARAMRVKDEFLASM
                     SHELRTPLTGILGFSEALQLNAYGELSEKQLKPIKAIEDSGRHLLSLINDILDLSKIE
                     AGKLELQFATCSITDVCTASLQIVKGMAQHKRQTIHYSPTNQPVAIFVDARRIKQVLV
                     NLLSNAVKFTPEGGELGLDVEVSAKEQVARLAIWDKGIGIKPEDLPKLFKPFTQIDSS
                     LAREYSGTGLGLSLAQRLIELHNGKITVESSPGQGSRFTVTIPWSEQNDAQLEQTTDQ
                     KNSLFSQVKSEDSSPAVIMIADDNVAILNLLEDFLTPRNYKIAKAHSGLELLERITES
                     HPDLLLMDIQMPGMDGLETIRHIRSHANPKIATTPIIAITALAMPGDRERCLQAGADE
                     YMSKPLKLYELSIIIKKYIRKSK"
     assembly_gap    20579..20678
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20679..22510)
                     /locus_tag="PLE39_09005"
     CDS             complement(<20679..22510)
                     /locus_tag="PLE39_09005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE68506.1"
                     /translation="MDEDIHILIVEDILDDANRVMQEVRKSIPGCQFEHAINEETFQS
                     ICNRFSPDLVFLDHQVTGFTTLEAFKSVKKSWPLTPCIVYARSFDEENAVEYIKAGAA
                     NYVTQNNAKRLGLAALKALEEKKELIEQSRVEQAMLESEEKFRSLIEQSSDGIVMIDE
                     HGMIIEWNSAESTITGIPRERAMGAPFWEIQYEILTPERRAQISPEEFKSHYFEVVRS
                     GNPELINKKFEIDFMTAQGEIRVLHQSSFIVTSQNGSRIGAIIRDVTEKKHAEERLQR
                     DRQILHEAQVMAKLGSWSVDLRTGEFNIDSECSTIAGCPPGLRDLSEILSLVHPEDLE
                     NARSAWQMLQQEGAPADLQLRITSNGKIKWLRIKAKLSAAKNDIPMSAIGVIQDITDT
                     KTAEQILHKSEIRLATVFRVSPISMAITRMKDNCLMDVNEAWEEITGYRKEEVIGRSV
                     LEINSWADAAERQQLVTLLRDRRNVQNFEFKLRHRSGEIRQLLMSAELLDLAGEECMI
                     TLALDITERKKTEYALQASEERFHKAFYSGPVGLAITRASNSTYIDANEAFSNIVGFS
                     REELLNQTSLGLGITTAKQKDEYVKLIREQGYIRDKEMELRHSSG"
     gene            complement(22539..>25067)
                     /locus_tag="PLE39_09010"
     CDS             complement(22539..>25067)
                     /locus_tag="PLE39_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE68507.1"
                     /translation="IEKQINGEPTEAEYRIKWPDDSIHWIWDRSFPVYDEEGKLTRTA
                     GIATDITLNKKAEEELKERESFLQSIVGGSPGYIVSHDIEGHFLYANRRWPEQSEEEF
                     RSMKLQNMLHPNSLEPVMEAFRKAFVTRETQRVEQSVLTPNGDYHWFDVIYSPQVVND
                     EVKSVTAFAYDINDRKLAEIALRESEEKYRGLMESLDNIIATMDYDGRFFYVNDKAAD
                     ALGNKANEIIGKTVFDLFPDEVSASLMENIRIAIQEDRGVLMESKNLVRGEFRWYRTM
                     IQPIHDAQGKVLHVLTNSTDIHDLKMAQHDLQELNNTLEERVQIRTAEVQDLYDNAPN
                     GYHSLDADGALVMINQTELNWLGYTRHEVLGKMKFRDLLGSENSSMFDGIFSQFVTSG
                     KIADQTLRLIRKDGSPLPVLLNAYAIYDEYGNFTTSRCTITDITERVKIENALRESRD
                     QLSAANVSLEKAARLKDEFLASMSHELRTPLTGVLGLSEALQLNAYGELNEKQLRTVK
                     AIEESGRHLLELINDILDLSKIEAGKLDMQFTSFSINDICQASLQLIKGMANQKRQNI
                     HFTPPTKPIMLNADPRRIKQILVNLLSNAVKFTPEHGELGLVVEVNPADENISMAVWD
                     KGIGIKTENIQKLFKPFTQIDSSLAREYSGTGLGLSLAHRLVELHGGRIELESVFGEG
                     SRFTVILPWSDEEALSVYGELDAPSQSQSNKTGETNPSMIMVADDNETVLEMITDFLE
                     SKQYRVTKARSGSELIQKVPEILPDLILVDIQMPGMDGLETIRLIRSSSNPTIASVPI
                     IAVTALAMPGDRERCLRAGATDYMSKPMKLNELAMLIQKRKRPQ"
     assembly_gap    25068..25167
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25168..>27276)
                     /locus_tag="PLE39_09015"
     CDS             complement(<25168..>27276)
                     /locus_tag="PLE39_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE68508.1"
                     /translation="RWIHQKAKIIYSKDGQPIRMMGTIQDITEYKQAQEDLQSAREFL
                     QGVQDALSAHLAILDENGVILQVNSAWRKFGKENGLDRPDACIGMNYLQVCDSAEGAD
                     AGEAKQVAQEIRAVCSGEKTAAWVEYPCHGLEEKRWYVVHITKFMDRGQVRIVLAHEN
                     VTERKMADEALRASEERYRLITSYVDEIVWQVNTDLRFVYASPAATRVLGYTNEEAVN
                     LHIYDLLSEEDAARMHGNIMARINHEPGVSNKPTEYRMRHKNGNLIETEALAAPIFDN
                     ANRLIGFAGITRDITERKVIERQLQEAERKYRTLVEYSPVVVYVDEIGGEWQYISPQI
                     KKILGYSPTDFLSDAQAFKKLIHPDDLHRFQIQVNESLKKGQSLKLEYRMLTRDGSYV
                     WIHDESDLTQSGDNGKYYLQGVFYDITERKQAEEALAYNERRFRALIENIADGIQLVD
                     MKGEVIYVSPSAARLLGYQQGEMEAPITSLIHPDDLNQVAASISKSMTTQEALMQSYR
                     VKHKDGTWRWFEGSITNRLTDPAIGSIVMTFRDVTERKRSEDALRESEENFHSLFEDS
                     PIAIWLEDFSQLKKYLDRLREQGVPNIKNYLNEHPAELERLSGMVNILEINKTSARIL
                     GAQTKAEVLKSLPDYFTKESLKVFADEIAALADGQTQFKSEIPVKDLYGKEVIFDLTL
                     TVQPGSEEDLSRVLVSFLDVT"
     assembly_gap    27277..27376
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27377..>29062)
                     /locus_tag="PLE39_09020"
     CDS             complement(<27377..>29062)
                     /locus_tag="PLE39_09020"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE68509.1"
                     /translation="IRLWGTAIEITDRKQTEEALNTSRQLLQTVINNVPVRVFWKDRE
                     SRYLGCNPSFAMDAGETDLQNIIGKDDYELSWRNQADFYRADDQKVMSTGVGKFFYEE
                     PQSTPDGKLIMISTSKVPLRDENGQTIGVLGIYQDITEQKQAEELILAQRDLAYIISS
                     ATSNKDAFKHCLAIALRISGFDSGGIYLFNEGKQTLDLVHHHGLSEKFVQSSSSYPVD
                     APNVKTLFSGLSFYFNDADLRDRETFLTEGIHSYASLPIQHHGQVIGCINVASHAMVD
                     VPDFTRRALEVTASEIGNIITHLQTEASLHESEEKYRSLINSQESSIAMLDAEGIYHY
                     VNEIGAARLNGSVQDIIGKKAQDLLPPETVEWKINVLQQVIETGQGIVQEVQLQEASG
                     KFWHRISVQPIRDANGQVKLAMLTSIDITERKKAEAELQNLKDRLLEAQQLAHVGNWE
                     WDTHKQVLFWSEEVYRIFGVNPAEFLPTVEAFEGAIHPEDKESFLAQRAKMLEEQQAA
                     SIDHRIILPDGRVRHVTEKTQLIKDKNGNITRIIGTVQDVTEQKLAQEALLKSQ"
BASE COUNT         6353 a         6855 c         7611 g         7943 t
ORIGIN      
        1 gaaagttata tcgggttggc tgtgaatttg actgatttaa ttgtgggcgg gcagtaattt
       61 acctgtaact tccccaaacc cgactcggta gccctcgccc tcgcaccaac ctgtgagtgt
      121 gagggcgtct ccgtcttcaa ggtaggtgcg tttttcgccg ctcggcatat tgagtgggtg
      181 ttccccgcgc caggtaagtt ccataaggct tccgtacgaa tcttgcgtgg ggccgctgat
      241 tgtgcctgag ccaagcaggt caccagggcg catgttacag ccgttgaccg tgtggtgagc
      301 aagttgttgg cgcattgtcc aatacatgta gcgaaagttg gagcgtgcaa tcgtaacggg
      361 tgagttcatt tgcttggttt gcagcgagac ttccaatttg atatcgtaag aggccgcgcc
      421 cttgctttgc aggtagggga gcggagttgg gctttggctg gggttttcgc atcggaaggg
      481 ttcgagcgca tccaatgtga caacccaggg tgcaatcgaa gtggcaagat tttttgagag
      541 aaaggggccg agcggttggt attcccattt ttgaatgtcg cgcgcgctcc agtcattgat
      601 gagtacgagg ccgaagatgt gttcttcggc gttttcaatg ggaatggggt gtccaagcgg
      661 gttaccaggg ccgacaaaaa atcccatttc gagttcgaaa tccatgttgc ggcttggacc
      721 aaaggtgggc ccatctgcgc cttcagcaat ggtttgccca ttgggtcggc gtatgtctgt
      781 gccgctgacg acgaccgagc ttgcccgtcc atgatatgca atggggatat gcacccaatt
      841 gggcatgagc ggattctcct tgctgcgaat catggagccg atattggtgg cgtgttcttt
      901 tgaggaataa aaatcggtgt agtcgccgat ggctgcaggg agatgcatgg ttacggtctg
      961 catcgggtgc aggacttgtt tttgtaagtc tggatcatcg cgcaggatgg ggttgtcatc
     1021 agaaagcagg gcttgcaagg ttgcccgtgt ctcatgccat acactacgcc caagcgccat
     1081 gaaagaattg aggttggggt gattgaagat gtgtttcgtt ttgaggtacc ctttttgctc
     1141 aagcacggca agatccagga cgaattctcc aatggcaacg ccaacccgtg gaccttggtg
     1201 ctcggcggtg ctgaaaacgc catacggcag gttttgaata gggaaatggg attcggggga
     1261 aacaggaatg aaggattttg acattaaacg gttttccttt ggaagcctga gaaatggttt
     1321 ttgtttctgc ctagcttaac caagtcaatt tttgcatatc ttcacgcggc tcgtcgaagc
     1381 tgcaactgcc ataggagatg aattgtgatc ttcgagagtc ggcgatctga tcggcagtga
     1441 cggagaaatt gccccatgtg aaatgtgatt cggtgaaacg aaaatgctga gggtcttctt
     1501 cgtttaggat cgccacggtt tgctcagtat tcaggttgtg tgcttgagcg aggatggcgg
     1561 ctccgaagac gttgacgaat ccgtgcattt ttgtctgtac gctttcgttg tagtggcgga
     1621 tggggtgatg caacccagcg gttgccttga atgggacatg atgattgcgg caaagcagca
     1681 gggcatgtgc gatctgttcc acactgggaa aggcgtctgc cacgacacct ccacagcgga
     1741 gtttgaatcc attgcggagg ttggccgttt ggtttgattc ggaaagggca atgattgttg
     1801 ctgtcatatt ttcacgccag gcaggggtga tggggatttc ataataggcg gtcagtttat
     1861 tttgcgaagt gattttctcg ttggcgtatt tgagcaggtc tggtgtggct tctgaaggca
     1921 ggcgcacttc gaacacatct gcaaaagcgg gttgatgctg acgaaaggat cgtatgtcgt
     1981 tcaagtcgtt ttcaaggttt tgcgcgaatt cgtcggcgtt ttttccgctg cggccaagaa
     2041 ctgaaaaatg ccacgcgtcg gaaaggtttg ggagtttgga taaatcagca gcggagataa
     2101 tgaatcggcc gagcatccag ttgtcggtgg agggaagata cgccttgtat tcatccaagg
     2161 ctgaagcgag atcgaggctg gcaggtggaa agaggcctgc atagtcgatc aggtttttca
     2221 gaaactcgcg cagtgatttt gcaacaggaa gagtcatgcg gctgatttta tcgcttgttt
     2281 tgaaaaaaga aacatcccga agggttttcg atttcccttc gggatgttgt atttaatttg
     2341 gatacccagg gttggtaggt ttgggggtgg ggcgcacggg cggctcaggc aggaaggatg
     2401 gtgtggggat gatattcagg tcgatcccag aggcatcgat ccattccaga tatagccagc
     2461 cgatcttttc atcgggcgct ttgacataga accaatcttt gtatttgctc agcacttcca
     2521 tttttgtgcc gcggatgtag gcatcggaaa tggtggggta atccacatca gggcctgacc
     2581 tgagataggc aaaatcaata ttgatgacga tgtacagcgg tgatggggtg gccgtggcgc
     2641 tgggtatggc cgtgggcgtt tcagacggaa cggggctggc ttccgcgggc ggttgggtgg
     2701 ctgttgcggg tggggtgcag gcagcaagga ataggattag aacagataca acgataaagt
     2761 aaaaagcgga ttgtttcatg gaattctcct ttacattttt tgcaagcata aagttgaatt
     2821 ccgtacctcg caatgaaaca taagtaatgg gcgcagcctg gttttattta ccgcggtaat
     2881 caatggtgtg agacggaatg gtaattcccg aggcagagac attaagcggg tgcgatttca
     2941 cgccagattc gggtgatacc ctttccatgg ctttttgggt cacgagaatt tcaccagcgg
     3001 cggcggtgtc ttctcccagt ttgcaggcgc ggttcacggc atcgccaaaa caatctttgt
     3061 tgccgataac cagtatcttt ccaaagtcaa tgccgcaaga gatgtgaaca tcgaaatcgt
     3121 cgggtgtgag caggttggag gcgtcgaagg catgttgcaa ggcgatggcg gcctggatgg
     3181 cgggaagtgt atctgggaag taggcaaagc agttgtcggc ttcaaatttg atgaccttgc
     3241 catggaacga agtgatgatc ggctctgaaa ccaactgcat acgccgcacc atggataaat
     3301 agtggatgat gccgtatttg cgtgtcaata atgagaaccc agacatatcg agcacgaaga
     3361 tggcgcgttc gctgccgtat tcattccata actcggcttc gatggcttcg cggttgacat
     3421 tgttgacttc ctgcgaatat ctcaagagca ggttttgaaa tttggctggt agttttcggt
     3481 tcatgcagtg agaatacaac caaaggctca ttttcgcaag ccagttccat gacaatgccc
     3541 tcccagtttt gggagggcat ttttctaaat atccccaggt tcatcggcca tgcggacgat
     3601 tttgggcgag aactttccaa cgatttccac aaggtcattt tgtgcggcga tgacatcttc
     3661 gatctgtttg taggcttgcg gagattcgtc gaggccgccg ccgaggaggg tgactccacg
     3721 ctccttgaga tagacatccc gcgcagattt gctgatcgaa tcgatggcga ctctgcggct
     3781 catcttccga cccgctccat gcgaggctga agaaagggaa ttctccaccc ctctgccgcg
     3841 gactacgtac cctgcatcgc ccattgagcc tgggataacg ccgagcaccc ctttgcctgc
     3901 gggtgtcgct cccttgcggt ggacgatgac tttgcgccca tcggcgagtg tttcatgcca
     3961 ggcaaagttg tggtggtttt ccaccgcagc cacttccttc agcccgatgg cggcggccac
     4021 gcgtttgtgg atgatgtagt gattggccga agcgaatctg cccgcgagtt ccattgaaag
     4081 ccagtattcc tgaccttctt cagaatcaag cgagagccag gcaagatgtt ttacgctctt
     4141 atcgaggttg ggatgcttct ccatggcgag cttgctgaag cggtcggcaa tttttgcgcc
     4201 gacgccgcgt gacccgctgt gcgaaagcag ggccaggtat tgaccagcct tcaacccgaa
     4261 tgtgtcttcg tgcagtttga aactgcccca ttcaacaaag tgattgcctg tgccgcttgt
     4321 gccaagttga ttcatggcgg tgtctttcag ggtctgtaat tggcgggtgg caaaccaggc
     4381 tgaatcatcg agtacttcat gctgggcttt ttttgcgcct gtccattttg cgcccatgcc
     4441 gaaagcggtc tcgttccaga gcgattcttc gaaaaggcct ttcttttgtc cgagtaaatt
     4501 tggtgaaacc tcgtagaccg agagtctcat gcggcaggca atgtccacgc ctacggcgta
     4561 ggggatgacg acattttcag tggccagcac gccgccaatg gggaggccgt agccaacgtg
     4621 ggcatcgggc atcaaagcac ccgtaacggt gacaggcagg cgcatggcgt tgttcatttg
     4681 cttgacggct tcgtcgtcaa tattgtcccg accccaaatg gggaagggca gtggaccttc
     4741 gcgcaaagtt tcgggtttgg gttcatcctt ttgcatggtg cggatgcatt cacgggcgag
     4801 atcgacggtc actgtatcgg cgaggaaact gccagggttt tgacgaacag cttccagttg
     4861 agccagaacc gcatcgcgtt ccattccttg ctccatcagt tttgcggcgg cgtcttttgc
     4921 aagcccgatg attcttccgt cgggccagtt gttgagtttc aggatttttc ctgtgatgat
     4981 gtccattttt ttaattctcc gacatgtcac tggaatgaca tgtcttgcta gatcgggtcg
     5041 ggcgggtcgc agggaccttc gcaggtgagc gtggtgaata cttcggcttg aagcatccat
     5101 tcaccgcttt ttgcgcgctg ccatttggcg gcatagacgc ccgacgcgtg aaccaaaatt
     5161 ccttcagcag tataacttcc ctgccaattg ccgatttcct gtgcaagccc ccagctttcg
     5221 ttggtggtga cctcgcgcgg ggttcggcga tagatggcgg ttgggtcggt tcggaaaagt
     5281 tcagaccaac gtatgccttc ttcttcagct ccgtggaatt gtgcgctgcg gcccgtaatg
     5341 aggtggtagt ttggcgccag cagggatgtg atgatatcgg cgtcttttgt ggcgatggcg
     5401 ctgttgaagc gttcgcgggc ggcgtggatc atttcggttg aagcaggttg ggtcataatg
     5461 tttttccttt ttgctcaatg gtctattaat ttcggtaatt attcttccag tttccacaac
     5521 tctcgcattt cctggctggt tgtgagtaga tggtcgagtg tgccttcaga ttcaaccttg
     5581 ccgtctttca gcacaataac gtgatcggcg cgccgcaaca gcgggcggcg atgggacacg
     5641 acgaggcagg tcaattggtt gcctgcggcg aagatgcgct cccatagttg actttcggtt
     5701 tccacatcca gcgcgctgga gagatcgtcg aagacgacca attcagattc gcggatgaac
     5761 atgcgcgcgg cagcggttct ttgggcctgc ccgcctgaga gtttgactcc acgtgcgccg
     5821 accatggttt cgaggtcgtc gtcgagttgc tccaggtcgc ggtccatgac ggcgagtttt
     5881 gtggctttgt aaatttcctc gtcgctgcgc tgcatgccga gcaggatgtt gttccgcagg
     5941 gtgttgctga acaaacgtgg aacctgcgcg gtgtaggcac agcgcggcgg gatgaggaag
     6001 tcacctggtg cggtgaggat ggtttcgttc catcgtatct cgccagattc gatcgggagc
     6061 aggccgagca gggcgcgcag aagtgtggtt ttgccagagc cgattcggcc tgtgatcact
     6121 gtcagcgagc cgcgattgat cttcagggaa atatcctcga tgccgttgac cgaatcgggg
     6181 tagtgaaatg tcagatggtc agcggacagg ctgcggagtg agtcggatgc ggtttttgtc
     6241 ggatagatca cttcgggcag gggaccatcc aggtctattt tgctgtgttc aacgagcgcg
     6301 ttcagcggtg cattttccat caggtgatac atgcgctgta cggagacgtc aagctgtttg
     6361 tagcgggctg cgagcatgcc gccgaaagtg gtgaggtcgc ccatgctttg caataaataa
     6421 acgaagagcg aaaagtctcc gagcgtgaaa ttgccctcgc gcatggattg cccgacgagg
     6481 ataaggatga cgcccgtgcc aagcgtggac atgttacggt agatcgagcc aagcacttca
     6541 tcgaacagtt tttcgcggat ggtgagcaca cgcctatcat cgttgagttt gtgaaagtga
     6601 tggatgacat ctttttcggc tgtggctact ttgacagcct gcacggcgcc aaagaactcg
     6661 ccgatgaagc ccgtcacttt gcctccagcc tgccgcgatg atcggcggta gtgttcgatg
     6721 cggcccgtgg ccagattggc aacgatgccg acgagcgcca gcggaatcag cgcgatgacc
     6781 gttacggagg gactgatctg cgtcatcaag atgatcgcca cagcgatgat gattagcccg
     6841 accatgatgt cattgatcaa aatgacgaac agcggaattt catccacatc ctttttgaag
     6901 cggctgacag cttcgccagg tgaatcaggg agttgggatg cgcctggccg tttcaaaatg
     6961 tagcgcaaaa gattttttcg cagcaacgtg ttcatttctg cgaaaatggg cacgtcggca
     7021 tggtaaaacc cgtagcttgc cactacacgt ccgatccatg tggcggcgag gaatgcgaca
     7081 atggcccaaa tgccaaaggt caattgtgct tcgcctgtga ccatgtcgaa gaaagccttg
     7141 atgatgagcg cgggcgccac ctgccagcag aagcggatca atgccacgct gaacaggtcc
     7201 acaacccaaa gccacgggcg gaagcggacc atttcgaaga tgactttcca cgctgggagc
     7261 gcgggtactt tttgagtgaa ggttgtttcg gtcataataa aattccttgc ggtctttggc
     7321 cgcaacgctg ggcatggacg ataaacggtt tgccgagtaa cgatctgctt tgtcaacggc
     7381 tggctatgcc agcacttctt ccatgcctgt ttggagcaat tgataaaaac gcgagttcaa
     7441 gtcggcggcc aattcttttc gattgccgta ttcgctgact ttgcctttgt ccaggatcat
     7501 gacattgtcg gcacgttcga tcgtgtccag ccgatgtgcg atgatgatgg ctgtgcggtt
     7561 tttcaatagt ttgtcgatgg cgcgttccaa cttctgttct gtggcagggt caaggcgcga
     7621 tgatgcttcg tcgaggatca ccaggcctgg atttcgtaaa aagacccgtg tgaaagccag
     7681 caattgcgct tcacccgcag acagggaacg ggaacctgtt tcgagttcag tatccagccc
     7741 tttgggcagg ttctgatacc aatcgccgag ttccaattcc tcgagagtcg caatgatctt
     7801 ttcatcggat atcgtgcggt caaaaaaggt caggttatca cgaatgctgg cacgaaaaag
     7861 ctgaacatcc tgagtgacga tggcgatatt gcgccgcagg gtttcgatgt gtgcatcgcg
     7921 caagtctgcg ccgttgacct tgatgctgcc tgcgtttaca tcgtacagac ggaagatcaa
     7981 acgtccaagg gtggtttttc cgctgcctgt gcggccgagc agcccaagta cggaaccagc
     8041 tctcaggtcg aaggaaagac cgttgatgac tgtatcctcg ccgttgtagg agaaggtcac
     8101 atcttcgaag gtcaattcaa gcggacgtgc tggaatctcc acgccgaccc cgctgacaac
     8161 ctccgctttg aaattgcgga attcggtcag gcgttccaca caggcgccga tggtctgaaa
     8221 actttcgatc tcgtgggtca tcgcccagaa tggttcttcc agcagattga tgtagtgtac
     8281 gaagagatac accgtgccga tggtgaccag acccgcagag aacagccaat atccgctgga
     8341 gatggcgagc aggtttccag tcgtcaatgc aaaacccatg gcgttttcga tgatccagcg
     8401 tttgaaatgt gccttgcggt cgtgggtcaa cttgatgcct tgcaaacgga atagttctcg
     8461 aatggaataa tccacggcgc cgctggagcg gatatcttct gtgcctgcca gttgttcctc
     8521 gataaaaccg aagatctgtg catccgcttc tcgcctggcc ttttgatgcg gcacggcgat
     8581 gtctttgagc cttccgagaa caaagatcgt gaggattgaa taaacggcga atgccagccc
     8641 ggcgcgccag tcttcaagga aaagcgccac gaggattcca acaagcagca agccattggc
     8701 gatcaaattc agcgcgaact gtgaaaagaa ggttgccagc tcggtcacgt caccgtcaat
     8761 gcgctcgatc aattcgccgg gcgtgtggtc gttgtggaat ttcatatcaa ggtgcagggc
     8821 gtgctccgcc agttcggcgc gcaggtcgtt ggtggcggtc caggctacat tctcgccgag
     8881 gtaggtcacg cttacagcca cggcctgttg gatcaaggca atgccaatga atgcaatcgc
     8941 ggtccacatc agcgtggata gggcttcgcc agccagcgcc gagtcgataa agcggcgcat
     9001 gatctgcggg gcaaagattc gcaagccgat actgccaaac aataaagcgg cgagcagaat
     9061 gaaacgtccc ttttgtgggc ggatgtggtc ggagagtaga ttccagtaag atttgaatga
     9121 taggttcatg atgttttgtc cattttgaca ataaaaaaca cggcgcaggt gcgccgtgtt
     9181 tggaaagaca cagaaagttg aatccgtggt taacggtcaa taggcgcact tcgagatgat
     9241 tgaattattc ggtgtagttt ggctgtaaga ggcaaattca tttgaagtgc gttcctttcc
     9301 tggagtttag tgtttgcagt gtacaacact tgcgcattgc ttgtcaagga aattacattg
     9361 cgcaattcaa tcacaggcat tgatcaggtt tgcaaaacca ccaaacggac tacaattgaa
     9421 gcccatggag gctttatgaa aaacacatat cggatgcgct attggcgtat cgttctgttc
     9481 tttgcgcgtg tcaccctgag tattattttc tgggatatct ttctgccgcg cattggcttg
     9541 cgctttcttt caagaaatac gcgcccgcag cggctgcgtg gcattgccgt ccgttttcgc
     9601 gccctggcga ttcgacttgg cggcgtgatg atcaaggtcg ggcaatttct ctcggcgcgg
     9661 ctggatgttt taccccctga gatcaccgac gaattggcag gattgcagga tgaagtgccg
     9721 cctgtggatt atgaagcgat ccgcgtccaa accgaactgg agcttggttc tgccctcgaa
     9781 aagatatttc tcacgtttga aaaagccccc attgccgccg cttcgttggg acaagtccat
     9841 cgcgcccgat tgttcccgca tgaagctgag acaattggtt ttgaaaatat cgtcgtgaag
     9901 gtgttgcgcc ccaatatcga gcagatcgtg gatgtggata tgtctgctct acgggtggtg
     9961 ggaggctggt tgaagcgtta tcggcctgtg agtgaccgcg ccgatgtgcc tgccattgtg
    10021 gaggaatttg ctgccaccat ccgcgaagaa gtggattatc tttcagaggg gaaaaatgcc
    10081 gaggcttttg cgatcaattt caagggcgat gagaacgttt atgtcccgcg tgtagtgtgg
    10141 acccgttcca gtcgtcgtgt gcttgcgttg gaggatgtat ctgcgatcaa gatcactgat
    10201 tatgaagcca tgactgccgc gggaatcagc cgtgccgcag ttgcagaccg cctgcttgcc
    10261 acatatttga agcagatctt tgaagatggc tttttccatg ccgatccgca ccctgggaat
    10321 ttgtttgtcg cgccgctgca cgaaaaaaat gcagatggct caacgaaatt ccgcctgaca
    10381 tttattgatt ttggcatggt cggtcggatg cctgagcggt tggtggaggg gttgcgggag
    10441 gctgtcatct cggtcggcct gcaagatgcc acccgtttga tcaaggcgta tcagacattg
    10501 ggcgtgctgc ttccaagtgc gaatgtgaag ttgttggagg aggccagtgc ccaggtcttt
    10561 gaccgttttt ggggtatgag catgaacgaa ctgcgcagca tccgtcacga tgagatgatg
    10621 aagtttggtc tgcaattccg cgagttgatg ctgacaatgc cgtttcagtt gccagaaaat
    10681 cttttgctgc tgggcagaac gatcggaatt ttatcgggca tgtgtacggg gttgaacgcc
    10741 gatttcaacc tctggctgca acttgcacct tacgccaaga agttgacaga aggcgaaaat
    10801 ggctccgctt ttgatgtgtt tctgaatgaa gcaggcgaac tgctcaagac tttgatcgcc
    10861 atccctggca gggcagagcg tgtgttgggg cgcattgaac gcggtgagtt aaacatgcaa
    10921 atgccgctgg tcaatattca gatcagctac ctggaacgtt cgttaaaccg attgacggga
    10981 ggagttgttt ttctcgggct gttggtttcg ggcgcgattt tatatgatgg gcacgctttg
    11041 ctggcacagg gaatgtttgg cggggcggtt ctggctttgt tttatacaac ttttcttgcc
    11101 agaggcaagc gcccatttca atgatcggtg ctttgcatat ttcaacttac aaaaaaactc
    11161 ccagcccttt ggctgggagt tttgcttatg aagatttttc ttctgttctc ttgatcgcgg
    11221 catcaatcag gctgcggaat gccagaagtg cttccttgcg ggcggcacgg cggtgctcaa
    11281 ggaagccagg tggaaggatg gtttccatgc tttttcgcat ttcctggcgt gcagctttca
    11341 tgtgttccaa cgcctcaggc ggaagtttat ctctcatgga tttgcgtttt tcttcgttca
    11401 taggtcactc cttttgtctt ttctgattat agcaccgttg gtactgggtg ggtgttttcc
    11461 acctgctaat cgcttacaat gggcgaaaaa agaggagcca atgagaaatt cattcacact
    11521 ttcgatcaag ttgatattta ccctggcggc tattttcgtg ttgaccgcat gtggtgtcat
    11581 cgcacccgca tcaccaacac ccgatgtgct cgccaccgtt gtggcccaaa cgctgacttc
    11641 catgccgaat cccgaagtag cagcatccac ctccacacct gttgccgcta catttacccc
    11701 gatcccatcc acgtttaccc cgattccagt ttcggttgca gctacgtcaa cgccgccgat
    11761 cgggggggcg aatttctttt ttaccagtgc cgagaacgtc aatctgcggg taaacccggg
    11821 caggctgttt caggtcagcc gtgtgttggc aaaaggaaca aggttgcaag ttttgggtat
    11881 ggccccaggc ggacagtggt ttaacgtcgt aaatgacgaa ggcatcatcg gctgggtcgg
    11941 aaaggatttt ctggaaggca accccaacca gcaaaacctg cttgtaatta ccccaactga
    12001 tgttctgacg ataacagggc aggtcctgga tgtgaatgga gtccctgtca gcggaattgg
    12061 gtttgccgct gttcaaggtg acaaacggga tgatggcgtc acagacgcag cagggatgtt
    12121 ttacatcttc ctgccctcca agtttacagg cgcgtggaca acaagctttg tctcggtagc
    12181 ctgtccaagc accaccatgg atgccaattg caattgtctc aatgggacat gcggaaagcc
    12241 cgaccccgag gttgttacag ggatgttgcc gctttctgca ccattgagct atgtctggaa
    12301 gtaaacggag taacataaac aaaaaacgct gccaattgtg gcagcgtttt ttgttttgtg
    12361 accaaccaaa taaaggttat ttcttctttt cgagcttcgc ccaggtatct ttcaaattga
    12421 cggtcaaatt gaagacgggt ttttcaggcg tagagtcggg gtcggcgcag aagtatccca
    12481 cgcgctcgaa ttgaatattt tcgccagttt ttacctctgc gagtgcgggt tctacaaagg
    12541 cgttttcaat cacatccagt gatttggggt tgatgatgct ttccaattca ccatcgtcag
    12601 gcctttccac ggtgaataac tggtcgtaca tacgcacttg ggcaggaatg gcatgatctg
    12661 cagaaaccca atggatcgtg gatttaacct tgcggccatc cggtgagtcg ccgccgtgtg
    12721 tggcagggtc atacgtggcg tgaacttcca ccacttctcc ctgatcgttc ttcactacat
    12781 gcgtgcattt gacaaaatag gcgtatcgca ggcgcacttc gttgccagga tacaggcggt
    12841 aatacttcgg cggcggagtc tctcggaaat catcctgctc aatgtaaatg gtcttcgaaa
    12901 atttcacctt gcgggtgccg gcgttcggat cttcggggtt gttgaccgca tctaattcct
    12961 ctgatacgcc ttcggggtaa ttgtcgatca ccaatttaat cggattcaaa acagccattc
    13021 ggcggatcgc gcgtttgttc agatcgtcgc ggatgacggc ctcgaattgc gccatctcca
    13081 cccagctgtc atttttggtc tcgccgatgc cggttacaaa attgatgatg gcttcaggcg
    13141 tcacgccccg gcggcgcaat ccacgcaggg tggtcagacg cggatcgtcc cagccgcgga
    13201 caatgcctgc ctccacaagt ttgcggagct tgcgcttgct catcatggca taggtcaagt
    13261 tcaggcgcga aaactcgatt tgcctgcttg ggaaggcttc caatttttca agaaaccatt
    13321 cgtacaatgg gcgatggata atatactcat ttgaacacag tgagtgtgtc acgccttcca
    13381 tcgagtcgtt ctgtccatgc gaccaatcgt acatcgggta aatgtgccat ttgtcgcctg
    13441 tgcggtggtg atggacgtgc ataatgcggt acatcacagg gtcgcgcaac aaaatgttgg
    13501 ggtgcgtcat atcgattttt gcacgcagta cacggctgcc ttcagggaat tcaccgttct
    13561 tcatgcgttc caacaagtcg aggctttcct cgatcggtcg gtttcgataa ggagaatcaa
    13621 ccccagggcg aatcgcagac tcggtttcac tccactttgt ccctttgggc aatgtacctc
    13681 gtccagcact catctcttcc tgagtctgat cgtcaacgta tgccagatcc attttgatca
    13741 ggcgcaccgc ccattcgtac aacaggtcaa agtaatctga tgcaaaggtc actttggccc
    13801 attcaatgcc aagccacttc gcatcgtctt cgatggcatt tacgaattca gtttcttctt
    13861 ttgagggatt cgtgtcatcg aaacgcaaat ataattcgcc gccaaattcc tttgccgtga
    13921 aataatcgat caagaacgcc ttgatgtgcc caatatgcaa gtatccattc ggctcgggag
    13981 gcaggcgtgt gcggatatgt ttgaaacgcc cactttttag atcctcagca actgcctcgc
    14041 ggatgaacgc ggggatacga cttacgactt cttctgaact catggatggc tcaaccttcg
    14101 ctgtgatttt atgcggatga aaaccgcttc tttcataacc tcaacctgat cttgcttcag
    14161 gaacattttc ccacccgaag ccgtcacagg cgaaaagcac gccaattata actgctttat
    14221 aataacccta ccctggagga acccatgact gaatcaaacc aaaatcggac caattttgca
    14281 ggcacatact ttgaccgtga tgccgttctg cggttgactc gtttgttgaa gatcgcttcc
    14341 tggatcgtgg ctgtcgccta catctttcaa ttcatcacca acatctcagt gttgatcctg
    14401 cagatatcac gagacttgat ctatattggc ggcccaaccg atctgatgct gcaaattgtc
    14461 tggcagatac agcccagcct tgctggggtg attttttatg ttgccctgca agccattgct
    14521 cagaccttgt tgatcctgct tgatattgaa gacaacacgc gccgcgccgc ccgcaagtaa
    14581 accataaaaa ataaaccttg cttcatcctg atgggcgtga taaaataccg cccgctggaa
    14641 gaatagtggg gcttgtctaa aagcagaaca ggtaaccctg aatcagctgt ttaggcattt
    14701 tggggagtgg tgaaatggca tcacgttgcg ctctggacgc aaaatttgag gttcaaatcc
    14761 ttgctcccca gcttgaaagc aattgctcaa aaggcaattg cttttttttg tatccctgcc
    14821 gtctgatttg cgtataggca ttttgagtta tataggatgg atataattac gtctgatcaa
    14881 aatccaagtt ccaaacttgt tttggaacaa aataaggaaa agtcatgatt gtcaaagctg
    14941 tggaagcaga tggtgaaaag attcaggata ttactgcaag ggcgggtgtt ttcaatgagg
    15001 aagaagtgga ttgtgttcgg gaaatttggg atgaatacat cactcttggc ccggagcaat
    15061 gtggatatca gtttattgtt taccgagatg gggagcaggt tcttggcttt gcctgtcatg
    15121 gcccacgaga cctgacaagc ggtgttttcg atctttactg gatcgcggtc gatccttcca
    15181 cacgtcgaaa cggagtggga cgtgccttgt tgaccgccag cgaggatgcg gttcgtaaag
    15241 cgggtgggcg aatgttaatc gctgaaactt ccggaacgcc tcattatgag tcgactcgta
    15301 atttctattt tggcatgggg tataaagacg aggcaagaat caaggatttt tatacggttg
    15361 gcgatgatct agtgatcttt atcaaacgca tttaaataaa tatccaccat ttgcactgca
    15421 gttaatttga cagtagcgta aatggtggat ataaaattgg cgtacttgtc tttttgaaaa
    15481 accctgaaaa aattaaggca cctggctgtt tccttcgatt tcgtccttca caagttctgt
    15541 gaagatcttt acgagttcgg ggtcgaaatg tgtgccagat tcactgcgaa tatagtcaag
    15601 cgcatcagct ttggacattg cgcttcgata ggggcgatct gagcataaag catcccatac
    15661 atctacgatt gcgaaaagac gcgctgaaat tggaatcgcc tcgcctttga tcccgcgtgg
    15721 atatccgctt ccgtcccatt tttcgtgatg acaataggga atatccaggg cgggttcgag
    15781 gaaatcaatg gatgaaagcc attgataggc atagactggg tgctttcgca tgataaacca
    15841 ttcttcatcg gtaagtttgg cggctttgtg gaggatggca tctggaatgc ccaacttgcc
    15901 tacatcgtgg agcagagagc ctctacgaac gtgttcaagg tcagcttcag aaaacccaac
    15961 tgccttggcg aaccgaatgg tcaggttcat cacacgttgt gtatgacctt ctgtctccct
    16021 gtcgcgcagg tccagcgctt gcgaccagcc ttcaatggtc ttgttgtaag caaccaggag
    16081 ttgtttgttc agggtctgta attcttcctt ctgttctgca atgcggcggt agcgattgag
    16141 tcgaaggatg gtttgcaggc gggcaaataa ttccatgctg ttgtatggct ttgtgacaaa
    16201 atcatccgcc ccggccctca gcccttggat gcgcgattcg tgatcatcca gcgacgtgat
    16261 caaaatgatg ggggcctcgg cgatctccgg catggaccga atgctcctac acacctcaaa
    16321 accattcatt ttaggcatca tcacatccaa taggaccaca tctggatgca ggttgactgc
    16381 catggatatc ccgtcaaagc catttgaagc gaaataagtc tcgtatggtt cacttgccag
    16441 caggccttcg actgcagcgc gggcaacagc atcgtcatca atgatcaaga tctttgggcg
    16501 ctcattgtgc attttctttc tcctttccat gcgttagtac aaacaatcag gatgtgatca
    16561 acccttgatt gatggcgaat ttcaccagat cggcatgtga agtgatgcgc agcttttttg
    16621 tcagattgag ccgatgcgat tcgactgttc gtacgctaag tgacaacttt tccgcaatgg
    16681 tggcgttgga atttccttca gcaaccaatt gcagaatttc ccgttcacgg ggggatagca
    16741 gatcaagtga ttcatccttg cctgcttcgg cactcaaaag catttccaat acttcgtctg
    16801 caatggcagt gctgaggtat cttctgcctt ccaatacatg ttcaacagcg tttaaaacct
    16861 cggtgaagtc agaatctttc aagacatatc cgagtgctcc actttgtaat gcgcggacgg
    16921 catacgtggc atttgcgtgc atcgataaaa agatgattcg tgtctttagc gcccgctgat
    16981 gcacaagttt ggcagtttca atgccattca ggttgggcat catcacatcc agaaacgcga
    17041 tatccggtga caagctttct attaatttgt aagcttcctg tccgtcgctg gcttctccaa
    17101 ttacttcaaa acctggctgg gcctcaagca gggatcgaac cccctggcga accagtttgt
    17161 gatcgtctgc aagaacaatt cttatgtgcg ccatgttttt cgctcctttc cattcagttc
    17221 aggttgaatt cggcaaggat aaatgtccct ttgccgggag tggattgcag ggtaaagaat
    17281 ccgcctgcca tgttcacgcg ttccgccatg cccgaaagcc caatggaggc gctttgatcc
    17341 ttgcttttga ggtcgaatcc ctttcctgca tcggcgatgc tgacctgtaa atggttggtt
    17401 gtgacagtga ttgtgatgtt ggctgtgttc acttcagcat ggcgggcaat atttgtaagg
    17461 gcttcctgaa ccacacggaa aacggttgtt tcaatgactt tatcaaaacg gcgttcctcc
    17521 agtgggttga tgttatggtt gatcgtgatc atggtctgtt ttgtaaatcg tttgaacagg
    17581 ccgtccaaag ccgcgaaaag gccgaaatca tccagagtgg tgggacgcag gtttaatgac
    17641 atttcacgaa gcctttgaaa cagatcggca gtgacagaac gggcttcggc aacaaccatg
    17701 gacatgtcgc tttcttttcg ttcaaggatc aactttaatc ctgtaaccag ttgtccgacc
    17761 tcgtcgtgca actcaaccgc cacatggcgg cgttcatttt cctgcgcttc caatatctgg
    17821 cgcgatagta tgcgtaactg ctcgtgcttt ttggagagtt cattaagggc ttcctgaagt
    17881 ttttcacgct cctccatgag gtgacgatag cggtttacgc gtttgagtgt ttgcaggcgg
    17941 atatccagtt cgagcctgtc aaatggcttg gagagaaaat cgtcagcacc gacactaagc
    18001 ccgttcaatt ttgcctcgtg gtcatcaaga gccgtgatca tgatgatggg tacttccgcg
    18061 atcttttcat ctgaacgaat gtatttgcaa acctcatacc cgttcatttt gggcatcatg
    18121 acatccagca gaattacatc cgggtggatg cgttttgcaa gttccaggcc ttgaatgcca
    18181 tcttccgcca tttccacttg aaaatttgat ttttcgagca aggcagcaag ggttgtacgt
    18241 gcaatttctt catcatcgat aacaaggatg gtgtacggtt gttttttcat tttgacttcc
    18301 ttatatattt ctttatgatg atggaaagtt catataactt tagtggcttg ctcatatatt
    18361 cgtcggcgcc agcctgcagg caacgttccc ggtcaccagg catggccagg gctgtgatcg
    18421 cgataatggg ggtggtcgct attttggggt tggcgtgaga acgaatatgt cggatggttt
    18481 ctaatccatc catgcctggc atttggatat ccatcaaaag caggtcagga tgggattctg
    18541 taattctctc cagcaattct aaaccactgt gtgccttggc gatcttgtag ttgcgggggg
    18601 tgagaaagtc ttcaagcaga tttagaatcg ccacattgtc atcagcgatc atgatgactg
    18661 ccggtgagga atcttcagat ttaacttgcg aaaacaagct atttttctgg tcagtcgttt
    18721 gttcaagttg tgcatcgttt tgttccgacc acggaattgt gactgtgaaa cggctgccct
    18781 gccccggcga gctttctact gtgatttttc cattatgtaa ctcgatgaga cgttgtgcaa
    18841 gtgacaggcc caatccggtg cctgagtatt cccgtgccag gctgctatcg atctgggtaa
    18901 acggcttgaa taatttgggc aggtcttcag gcttaatacc tatgcccttg tcccaaatgg
    18961 cgagtcgggc aacctgctct tttgcagaga cttccacatc caaccccaat tcgccgcctt
    19021 ccggggtgaa tttcaccgca ttgctgagaa ggtttaccag aacctgtttg atgcgccttg
    19081 catcgacaaa aatcgcaacc ggctgattgg tgggtgaata atgaatggtt tggcgtttgt
    19141 gctgtgccat tcctttgacg atctgtaaac tggcagtaca gacatcagtg atcgaacagg
    19201 tggcgaattg tagttccagc ttgcctgcct cgatcttgga aaggtcaaga atatcattga
    19261 tgagcgatag cagatggcgt ccgctgtctt caatcgcttt gatgggcttc aattgttttt
    19321 cgcttagttc tccataggca tttagctgca gggcttcaga gaatccgaga atgcctgtaa
    19381 ggggagtgcg tagttcgtgg ctcatactgg caaggaactc atctttcaca cgcatggcac
    19441 gtgccagatc catatttgca ttgcttagat ctgctgttcg ctcttgaaca tgttgtgcca
    19501 gttgattccg ttcctccgca aggagttgtt ccaccttttt acgttcgatg atctcctgtt
    19561 gggcggtttg gaacaaacgg gcgttttcaa atgcgacggc tgcctgattg gcaagagtaa
    19621 cgacaaggcg ttcgtctttt tcgctgaagg catttggctt ttcgctttcg attgagatca
    19681 caccaaggac attgtcaccc agcctgacgg gcacatataa gcccgaattg atgccgtggt
    19741 atgtctctat atatcgcgga tcattgctga tatctccgtg acgtacaacc tgtgtatttt
    19801 taactgccca gccgcttact ccatcacttg attttgtgat cttgttatta aattggtgtt
    19861 ttactgcgac gtgttcatcc ttgttagtgg tattaggctg actgaaagcg agtaacttta
    19921 gggtgtcatt ttctggattg tagaggcgaa tggcagtatg gtgccagtcc atttttcgtt
    19981 caagtaattc gatgatcttt tgcccgatct cctccgggct aagcaactga ctgagtgcca
    20041 gcccgctttc gtagagcatt tccatttcca ccatgtgttg atgggtttca ttctccgttt
    20101 gaagccgctt tgtgatgtcg ctgaccacca caacggtgcc aattaattta tccccttcgc
    20161 gaatgggggt gacggtatgc tctatcggga tgggtaggcc gtttttatgc cagtaatgat
    20221 caacgatcga atggcgggct attccatcgt gaaaagcctg atataccggg cattcaactt
    20281 cagggtaggg cgatccgttt ggccgtgtgt ggtggcagat cggatggatg ggacggccaa
    20341 gcaggtcaac aacttcccag cctatcatcc gtgccatggt tgggttgata aaggtaaccc
    20401 tgccttgtga gtctgtaccg tagatccctt caccggccga gttgagaatg ctttccagtt
    20461 ggtggttgag gcgggatatc caatcttctg cctgcttgcg agaggtgata tccataatgg
    20521 agccttccag atattctttt tctggaaaat attgtgcaga taagaggcag gtacgtatnn
    20581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc tgaactatgg cgtaattcca
    20701 tttccttgtc acggatataa ccctgttccc tgatcaattt gacgtattca tctttttgtt
    20761 tggcggtcgt tattcccaga cccagggagg tctgattgag caactcttcg cgactaaacc
    20821 ccacgatgtt tgagaaggct tcattggcat caatgtatgt gctgtttgat gcacgggtga
    20881 tggcaagccc taccggccct gaataaaaag ctttatgaaa ccgttcttca cttgcctgca
    20941 gggcatactc ggtctttttt ctttcagtga tatcgagtgc cagtgtgatc atacattctt
    21001 cacctgccag atccagcaat tccgctgaca ttaatagctg acggatctca cctgaccggt
    21061 gacggagctt gaactcaaaa ttctgcacat tgcgacgatc tcgtaataga gtgacgagtt
    21121 gttgcctctc agcagcatct gcccaactgt tgatctccaa cacggagcgg ccaatgacct
    21181 cttcttttcg ataaccggtg atctcttccc aggcttcatt cacatccata aggcaattgt
    21241 ctttcatgcg tgtgattgcc atactgatgg gactgacacg aaatacggtt gcaagccgaa
    21301 tctcgctttt gtgcaatatc tgctccgctg ttttggtgtc agtgatatcc tgtataactc
    21361 caattgctga cattggaatg tcatttttgg cagcggataa ttttgcttta atgcggagcc
    21421 atttgatttt cccgttactg gtgatacgca gttgaaggtc ggcgggcgcg ccttcctgtt
    21481 gcagcatttg ccatgcggat cgggcatttt caagatcttc ggggtgaacg aggctgagga
    21541 tctcggaaag atcacgtaac ccaggcggac agccagcgat cgtgctgcat tccgaatcaa
    21601 tgttgaattc acctgttctc aaatcgacag accagcttcc aagttttgcc atcacctggg
    21661 cttcatgtaa gatctgcctg tctctttgca gtctttcttc ggcgtgcttt ttttcagtga
    21721 catcacggat gatggctccg atgcgactgc cgttttgact cgtgacgata aaagaacttt
    21781 gatgaagcac cctgatctcg ccctgtgccg tcatgaagtc aatttcaaat tttttattta
    21841 tgagttctgg attgccactt ctgaccactt caaaatagtg agatttgaat tcttcaggtg
    21901 aaatttgagc gcgtcgttcg ggcgttaaga tctcatattg aatttcccaa aatggggcac
    21961 ccatggcgcg ctctcgaggg attccagtga tggtggattc ggcggaattc cattctatga
    22021 tcatgccatg ttcatcgatc atgacgatcc cgtcactgga ttgttcgatc agggagcgga
    22081 atttttcttc actttccaac atggcttgct caactctact ttgttcgatc agctctttct
    22141 tttcctccaa tgctttgaga gcagccagac ccagccgctt ggcattgttt tgggtcacgt
    22201 aattggctgc gcctgccttg atatattcaa cggcattctc ttcatcaaaa gaccttgcat
    22261 agacgataca tggtgtcaat ggccagcttt ttttgacgga cttgaaggct tccaaggttg
    22321 taaaccctgt cacctgatga tcgaggaaga ccaagtctgg cgagaaacgg ttgcaaatgc
    22381 tttgaaaggt ttcttcgttt atggcgtgct caaattgaca tcccggtatg gatttgcgga
    22441 cctcctgcat gacccggttg gcatcatcaa gtatgtcttc aacgattaag atatggatgt
    22501 cttcgtccat ggtgtttcct tttcgggcac ggttggtttt attgcggacg ttttcttttt
    22561 tgaataagca tggccagttc atttaatttc ataggcttgc tcatataatc ggttgcgcct
    22621 gcacgcagac atcgttcacg gtcgcccggc atggccaggg cggtgaccgc gatgatcgga
    22681 acagaagcaa ttgtcgggtt ggaactggat cggatcaagc gaatggtttc caatccatcc
    22741 atgccgggca tttgaatgtc tacaaggatc aggtcgggca gtatttccgg tactttttga
    22801 atcagctcag atccactcct tgctttcgtt acgcgatatt gcttcgattc aagaaagtcg
    22861 gtgatcattt cgagtacagt ttcgttgtcg tctgcgacca tgatcatgga tgggttggtt
    22921 tcgcccgttt tattggattg ggattggctg ggggcatcca attctccgta cactgacagc
    22981 gcctcttcat cggaccatgg caggatgacc gtaaaacggc tgccttcacc gaagacgctt
    23041 tccaattcaa ttctgccccc atgcagttca accaaccgat gggcgaggga aagcccaaga
    23101 cctgttccgg agtattcgcg tgccaggctg ctatcgatct gggtaaaggg tttgaaaagt
    23161 ttttgaatgt tttcggtttt gatgccgata cccttgtccc atactgccat gctgatattt
    23221 tcgtcggctg ggttgacttc aacgaccagt cccagttctc cgtgctcggg cgtgaatttc
    23281 accgcattgc tgagcaagtt aaccagaatt tgtttaatac ggcgcgggtc agcgtttagc
    23341 atgattggtt tggttggtgg tgtgaagtgt atgttctggc gtttttgatt tgccatgcct
    23401 ttgatcagct gcaggctcgc ttgacagata tcgttgatgg agaaggacgt aaattgcatg
    23461 tccagttttc ctgcttcgat cttggaaagg tcaaggatgt cattgatcaa ttccaagaga
    23521 tgacgcccgc tttcttcaat ggcttttacg gttcttaatt gtttctcgtt caattcgccg
    23581 tatgcattaa gctgcaaggc ttcggaaagc cccagcaccc ctgtgagtgg tgttcgcagt
    23641 tcgtggctca tactggcaag gaactcatct ttaaggcggg cggctttttc aagagagaca
    23701 ttggctgcgc ttaactggtc tctgctttcg cgtaatgcgt tttcgatctt gactctttct
    23761 gtgatgtctg tgatcgtaca gcggctggtg gtgaagtttc cgtattcgtc atagatggca
    23821 tatgcattga gtaatacggg cagagggctt ccatctttgc ggatgagcct cagggtttgg
    23881 tctgcaattt ttccgctggt tacgaattgc gagaatatcc cgtcgaacat ggagctgttt
    23941 tcactcccga gcaggtcgcg gaacttcatt ttcccgagca cctcatggcg tgtgtatccc
    24001 agccagttca actctgtctg gttgatcatg accagcgcgc catctgcatc cagtgagtga
    24061 tatccatttg gcgcattgtc gtaaagatcc tgcacctcgg cggttcggat ctgcacacgt
    24121 tcctcgagcg tgttgttcag ttcctgcagg tcgtgctggg ccattttcaa gtcgtggatg
    24181 tcggtggaat ttgttagaac atgcagaacc tttccctgtg catcatggat tggctggatc
    24241 atggtgcggt accagcgaaa ttcaccacga accaggttct tgctttccat caacacaccc
    24301 ctgtcttctt gaatggcgat gcggatattt tccataagcg aggcagaaac ctcatctgga
    24361 aatagatcga acactgtctt tccaataatt tcattggctt tgttaccaag ggcatcggca
    24421 gccttgtcat ttacgtagaa gaagcggccg tcataatcca tggtggcaat gatgttgtcc
    24481 aggctttcca tcaagccgcg gtatttttct tcactttcac gcagggcaat ttcagccaat
    24541 ttgcggtcat tgatgtcgta agcaaaagcg gtgacacttt ttacttcatc attcacaacc
    24601 tgcggactgt agatcacatc aaaccagtga taatcgccat tgggtgtcaa tacagattgt
    24661 tcaacccgtt gtgtttcgcg ggttacaaat gcctttcgga aagcctccat cacgggttca
    24721 aggctgttcg gatgcagcat attttgaagc ttcatacttc taaactcttc ttcactttgc
    24781 tccggccagc gacggttggc gtaaagaaaa tggccttcaa tgtcgtgaga aacgatataa
    24841 cccggcgaac ctcccacaat ggattgcagg aagctctcgc gttctttgag ctcttcttcc
    24901 gcctttttgt tgagggtgat gtcggtcgca attccggccg tgcgggttaa cttgccttcc
    24961 tcatcgtaaa cggggaagct tcgatcccag atccaatgga tggaatcatc aggccattta
    25021 atacgatatt ccgcctcggt gggctcgccg ttgatctgtt tctcaatnnn nnnnnnnnnn
    25081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25141 nnnnnnnnnn nnnnnnnnnn nnnnnnngta acatcaagga atgagaccag tacacgtgag
    25201 agatcttcct cgctgcctgg ctgcaccgtc agggtcaggt caaaaatcac ctctttccca
    25261 tacaggtctt tgactggaat ttcactcttg aattgcgtct ggccatcggc aagtgcggct
    25321 atttcatctg caaatacttt caacgattcc ttggtgaagt aatctggtaa tgatttgaga
    25381 acctccgcct tggtttgggc gccaagaatc ctggcgctgg tcttattgat ctccaggata
    25441 tttaccatgc cggataaccg ctcaagttca gcggggtgtt cgtttaagta gttctttatg
    25501 ttggggacac cctgttcgcg cagacggtct aaatattttt tgagctgcga gaaatcctcc
    25561 agccagatcg caatgggaga atcttcaaac aaggaatgga aattttcctc gctttcacgc
    25621 agtgcatctt ccgaacgctt tcgttcggtc acatcacgga acgtcatcac gattgagcca
    25681 atggcagggt cggtaaggcg gttggtgata ctcccttcaa accagcgcca ggtaccgtcc
    25741 ttgtgtttca cacgataaga ctgcatcaaa gcctcttgtg tggtcatgga tttggagata
    25801 ctggcagcca cctggtttag gtcatctgga tggatcaggc ttgtaattgg cgcttccatc
    25861 tcaccttgct gatatccaag caggcgggct gcagatgggc tgacatagat aacctcgcct
    25921 ttcatatcca ccagttgaat gccatctgcg atattttcga tcaatgcacg gaagcgtctt
    25981 tcattgtagg ccaatgcttc ttctgcctgt ttgcgttcgg taatatcgta aaagactccc
    26041 tgcaggtagt attttccgtt gtccccgctt tgggtcaggt cagattcgtc atgaatccac
    26101 acataactcc catcacgggt cagcatgcgg tattcaagtt tgagcgattg tccctttttc
    26161 aggctttcat tcacctgaat ctgaaaacga tgcaggtcat caggatgaat taacttctta
    26221 aaagcctggg catcactgag gaaatcggtg ggcgaatacc caaggatctt tttgatctgc
    26281 gggctgatgt attgccactc tccgccaatt tcatcgacat aaacgactac aggagaatat
    26341 tcaacaaggg tgcgatattt tctttccgct tcctgtaact gcctttcgat cacctttctc
    26401 tcggtaatat cgcgggtaat gcctgcaaaa ccgatcaggc ggtttgcgtt gtcaaagatc
    26461 ggagcggcca gcgcttcggt ctcgattaag ttcccatttt tgtgccgcat tcgatattcg
    26521 gttggcttgt tgcttacgcc gggctcgtgg ttgatgcggg ccatgatgtt tccatgcatt
    26581 ctggcggcgt cttcctcgct cagaagatca taaatatgca agttaaccgc ttcttcattt
    26641 gtgtatccca atacacgggt ggctgcaggg ctggcatata caaaacgcag atccgtattc
    26701 acctgccaga cgatctcatc cacgtacgag gtgatcaggc ggtagcgctc ttcactggct
    26761 cgcaatgcct catctgccat ctttctttcg gtgacgtttt catgagcaag cacgatacgc
    26821 acctggccac ggtccataaa ttttgtaata tggacgacat accaacgctt ctcttccaaa
    26881 ccgtggcatg ggtactcaac ccaggcggct gttttctccc cggaacagac cgcgcgaatc
    26941 tcctgggcaa cctgttttgc ttcaccggcg tccgcacctt ctgctgaatc acaaacctgc
    27001 aggtagttca tcccaataca ggcatcgggg cgatccaacc cattttcttt gccaaatttt
    27061 ctccaggctg agttgacctg tagaataacc ccgttttcat ccaaaatcgc aagatgcgcc
    27121 gacaaggcat cctgcacccc ctgaaggaat tcgcgcgcgc tttgcaggtc ttcctgagcc
    27181 tgcttgtact cggtaatatc ctgaatggta cccatcatgc ggatgggctg gccatctttg
    27241 gagtaaatga tctttgcttt ttggtggatc caacgcnnnn nnnnnnnnnn nnnnnnnnnn
    27301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27361 nnnnnnnnnn nnnnnnctgg ctttttagca aagcttcctg agccagtttc tgttcggtaa
    27421 catcctgcac ggtgccaatg atacgggtga tattcccgtt tttgtctttg atcagttgtg
    27481 ttttctctgt tacatgacga acccgcccat caggcaggat gatacggtgg tcaatggacg
    27541 cggcttgttg ttcctccagc atttttgccc gctgtgccag aaaactttcc ttgtcttctg
    27601 ggtgaatggc tccttcaaat gcctccactg tggggaggaa ttcggcaggg ttcaccccga
    27661 agatgcgata gacctcctcc gaccagaaca atacttgttt atgagtatcc cattcccagt
    27721 tgcccacatg agcgagctgc tgcgcttcga gaagacggtc ttttaagttc tgaagttccg
    27781 cttcagcctt ttttcgctct gtgatgtcga tcgaagtcaa catcgccagt ttgacctgtc
    27841 cgttggcatc tctaatgggt tgcacgctaa tgcgatgcca gaatttcccg ctggcttcct
    27901 gtaattgcac ttcctgaaca ataccctgtc ctgtttcgat cacctgctgc aaaacattga
    27961 tcttccactc cacagtttcg ggagggagca ggtcttgtgc ttttttaccg ataatgtcct
    28021 gtacagaacc attgaggcgc gccgccccga tctcattgac gtaatgatag atgccttccg
    28081 catccagcat ggcaatggaa gattcctggc tgttgatcag gctgcggtat ttttcctcgc
    28141 tttcatgcag ggaggcttcc gtttgcaaat gggtgatgat gttgccaatt tctgaagcgg
    28201 ttacttcaag ggcacggcgg gtgaaatcag gcacatcgac catggcatgt gaggcgacgt
    28261 tgatgcagcc gataacctgc ccatgatgtt gaatagggag gctggcatac gagtgtatcc
    28321 cctcggtgag aaatgtttcc ctgtctctta aatcggcatc attgaagtag aacgataaac
    28381 cactgaacag cgtttttaca tttggggcat caacaggata tgacgaactc gactgaacga
    28441 acttctcact caggccgtga tggtggacga gatcgagagt ttgttttcct tcattgaaca
    28501 gataaatgcc gccactgtca aaccccgaga ttctcaaggc aatggcaagg cagtgtttaa
    28561 aggcatcttt atttgatgtg gctgaactga tgatatatgc aagatcgcgc tgggcgagga
    28621 tcaattcctc cgcctgtttt tgttcggtga tatcttgata aattcccaat acgccaatgg
    28681 tctgcccatt ttcgtcccgt agcggtactt tggaggtgct gatcatgatg agttttccat
    28741 cgggagtgga ttgcggctcc tcgtagaaga atttcccaac ccctgtactc atcacctttt
    28801 gatcgtcagc gcgataaaaa tcggcctgat tcctccaact taattcgtaa tcgtctttgc
    28861 cgatgatgtt ttgcaagtct gtttcgccag catccatcgc aaagctgggg ttgcagccaa
    28921 gatagcgtga ctctctgtct ttccaaaaaa ctcttacggg gacattattt atgacggtct
    28981 gtaacagttg tcgggatgtg ttgagcgctt cttccgtctg tttgcgatcc gtgatttcaa
    29041 tggctgtgcc ccataacctg at
//
LOCUS       DAOQJC010000047        28402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_723_length_28402_cov_10.273433, whole genome shotgun sequence.
ACCESSION   DAOQJC010000047 DAOQJC010000000
VERSION     DAOQJC010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 28402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28402
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_723_length_28402_cov_10.273433"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PLE39_09025"
     CDS             complement(<1..709)
                     /locus_tag="PLE39_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE68510.1"
                     /translation="MTIDVSAIRKQFPALDRPATFFDNPGGTQIAKHSLDRINRYLIE
                     SNANHEGAFATSIASDAVLDEAHRAMADFYNASSPEEIVFGNNMTTLTLHLSRSISRD
                     WKEGEEIVVTRLDHDANVSPWVLAAQDRGVKVNWVDFDVEDGTLKLDQLQAALERKPR
                     LLAVGYASNSLGTINPVQKIIQMAHDAGTQVYVDAVQYAPHGPIDVQRLDCDFLVSSA
                     YKFFGPHAGILFGKHELL"
     gene            897..2849
                     /locus_tag="PLE39_09030"
     CDS             897..2849
                     /locus_tag="PLE39_09030"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNE68511.1"
                     /translation="MITNPVTSETSLKSRPGLRRALLGVWSVVVLYIVYVLGEAIAPQ
                     FGNAMKASMAQNLLGISSLLQIGFYYIVDILLVLGFSVIGVMIVMHSSDDWFAIFTSI
                     FLITFGVRVTDLANAIALQPGTEFAGGLLLAMGDIGIVLFTMLFPDGKFTPRWLRFLA
                     IPLVVTMLGIYLFPSAPFFWDKLGQGYLLITTSWYLIGFFTLLNRYFRKATLAHKQQI
                     RWVFIGTLGPFLWFIIFQVGLSFLPKPAESSLTMTAILLVSRLLSIFLFLVLPLSISI
                     SIAQTKLFNIDLIINRSLVYGSMTLALGLTFAVMLGLISLVFKNFHQGDQSLVAATIF
                     SVSAGALFQPARKKLQRFVDRFFYHIEIDYQKTPSDITLSPDSSSTTPRFSSYNDLRL
                     IGRGGMAEVYRATSQTKNKPVAIKVLRSALASDDSFRKRFMREAEIIARLQHPNIVNI
                     MDFGDENGVYHIVMEHLSGPDLNNLIKQEGRITLPKTISILRGVADALDYAHQHGLVH
                     RDIKPSNVMLDSSTIPPRVVLTDFGIAKIVDSQTLMTNTNMLGTLDYIAPEQIQASAE
                     VDSRADIYALGIMTYQMLTGTLPFEQASTGALLLAHLNAPPPDARTIVRDLPRNVAHG
                     IQRAMAKKPESRPSTAGEFITILENA"
     gene            2984..3406
                     /locus_tag="PLE39_09035"
     CDS             2984..3406
                     /locus_tag="PLE39_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNE68512.1"
                     /translation="MFKKILLATDGSEHALHATQIAAELARNPTAKELRIVVAYDVIP
                     PYFGEPNMQFAINARMEEAQTILRKTIQTIGSIPCEIHTELIEGSAAEAIIDVAATRQ
                     SDVIVMGSRGLGKLAGLLLGSTSQKVVAYAPCPVLVTR"
     gene            complement(3396..>3985)
                     /locus_tag="PLE39_09040"
     CDS             complement(3396..>3985)
                     /locus_tag="PLE39_09040"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNE68513.1"
                     /translation="TVVIQAGGMSSRMGEDKALKPFLGRPLIQRVIDRLAPIADEVIV
                     TTNRPDEYAFLGLRLIADLKPGRGALGGLYTAVASAAFSQVAVVACDMPFASDVLFEG
                     ARRLMVEEDADVVIAKTDEGYEPFHALYRRETCLPAIEAAIEADQWKVISWFSKVKVR
                     ELLPQEVKAFDPSGLCFWNLNTPEEFAEAEKRASG"
     assembly_gap    3986..4085
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4104..5216
                     /gene="amrS"
                     /locus_tag="PLE39_09045"
     CDS             4104..5216
                     /gene="amrS"
                     /locus_tag="PLE39_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNE68514.1"
                     /translation="MSSLAKRLDGLTVEGKLYEALEGGDVRCLACGHRCLIREGRRGI
                     CQVRFNRDGKLFVPHGYVAALQSDPIEKKPFSHVLPGVNALTFGMLGCDYHCGYCQNW
                     LTSQALRDPASDVSAQFIREVSPQTMVDHAKRTNASVVVSSYNEPLITSEWAVEIFKA
                     ARANGLLCAYVSNGNNTPEVMKYIAPYLDAYKIDLKCMSDKGYRKLGGTLQHTLDGIQ
                     RAREMGLWVEVVTLVVPGFNDSNEELWEAARFLAGLSVDIPWHVTAFHKDYKMTEPDN
                     TDGNTLLRAAEIGREAGLRYVYAGNLPGNVGEYKDTHCPQCSYQLVRRSGYVIQSYQI
                     TGDGKCPKCGEKIAGIWPSDPSTVGLYGVGRPFSVW"
     gene            5313..7064
                     /locus_tag="PLE39_09050"
     CDS             5313..7064
                     /locus_tag="PLE39_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE68515.1"
                     /translation="MKRNLSVLLSLVVLASIMLAACGGGAATEAPATEAPAATEAPAA
                     TEAPAATEAPAAYEGMKVESPDCDYGGEFKSIEAVDEFTVKITLCVPDPAFPSKIAFT
                     SFAIQPAEYLESTGGNGDLLEKPIGTGPYMVESWERGNQLVLKANPNYWGEFSGAETL
                     VFKWGTESAQRLLELQSGTVDGIDNVGPDDFATVEGDANLALFNRPALNIMYIGFNNN
                     PQVEGFDNSTNPLANEKVRQAIAMGVDRQRIVDNFYPAGSEVASHFTPCSIPNGCVGD
                     EWYSFDPEAAKALLAEAGYPDGFETVLNYRDVVRGYLPDPNVVAQDLQAQLKENLNIT
                     LNIEVMESGAFLAAADAGQLQGLYMLGWGADYPDQTNFLGYHFGAGASKQFGDKFDDI
                     VEALDKGAQLASDAERAPFYEAANNAIRTHVPMIPVAHGGSALAFKASVEGAFASPLG
                     NEEFSVMNNGTDTFVWMQNAEPISLYCADETDGESLRACEQVTQSLLAYETGGTAVEP
                     ALAESYDVNADLTEWTFHLRQGVLFHDGSSLDANDVVTSLSIQWDAANPLHTGNTGAF
                     SYWSGLFGTFLNAPPAQ"
     gene            7127..8206
                     /locus_tag="PLE39_09055"
     CDS             7127..8206
                     /locus_tag="PLE39_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE68516.1"
                     /translation="MVTYILRRLVLSIPVLFGILFATFALARLIPGDPCRAMLGEKAT
                     KVVCDEFNHRHGLDRPILVQFGIYVNEIAHGDFGNSFRYSKPITELLAERLPITFELS
                     IAALAFSVIIGIPLGVIAAVRHNSWADVATMLWANVGVSMPVFWLGLMLAYIFSLTLK
                     DTIFQLPPSGRLSAGLITTPFFEVWGWQLQEGTFSFSLADFISRMNVFNSILSGNWEV
                     LKDASIHLILPAFALSTIPMALIARMTRSSMLEVLGQDYIRTARAKGLVRRSVILKHA
                     FRNALLPLATVIGLSLGGLLGGAVLTETVFNLSGVGRILYDGITARDYGIVQGFTVVI
                     AIFFVILNLIVDVSYAYLDPRIRLD"
     gene            8216..9172
                     /locus_tag="PLE39_09060"
     CDS             8216..9172
                     /locus_tag="PLE39_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE68517.1"
                     /translation="MTTQSVSPKKLDAEALSLDSVSLWQITRRRLFRRKSSIVGMVIL
                     GLLIFIALTAQWLAPYDPTLSMLDADPPQKVEKRASPCIHALGCPADQPQHIMGTDGN
                     LRDEFSRMLYGARLSLMIGFSTVTFAIIIGTVLGALGGYFGGWIDNIIMRVMDVLLAF
                     PSLLLAIAIVTVIGPGLINALLAIGIVSIPAYARVVRGSVLSVREMDYVSATRALGGN
                     TFEILFKRILPNALTPLIVQGTLGIASAILDAAALSFLGLGAQPPTPEWGSMLGAERN
                     QVFTAPHLVFYPGLAIMLTVLAFNLLGDGLRDALDPRLAHTS"
     gene            complement(9303..>9968)
                     /locus_tag="PLE39_09065"
     CDS             complement(9303..>9968)
                     /locus_tag="PLE39_09065"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNE68518.1"
                     /translation="TVLVYVLQLLSVAVFGYAVYEIDWLEVYGARFNEAIRMGELWRF
                     ITPVFLHGSMAHIFFNMYALLSIGSFLERHFGHGRFALLYFLGAFAGNVFSFLLTGEN
                     GYSIGASTAVFGLIAAEVIFFYQNRKLFGGQARQAISNAGFIIAVNLFIGLAPGIDNW
                     GHVGGLFGGAIFTWFAGPFWMVVGTPPDFHLQDRREMREIIVGAAAVCVIFGALGIFG
                     MFS"
     assembly_gap    9969..10068
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10069..10554)
                     /gene="gmk"
                     /locus_tag="PLE39_09070"
     CDS             complement(<10069..10554)
                     /gene="gmk"
                     /locus_tag="PLE39_09070"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNE68519.1"
                     /translation="MPTSFELRKPNPLLIVISGPSGVGKDSVVQRMKERGFPFHFVVT
                     ATTRDKRENETNGRDYWFVSKDEFARMIDENELIEYAIVYGDYKGIPKWEVREALASG
                     QDVVMRIDVQGAETVRKLAPEALLIFITCEGEDELERRLRERKTETADSLSLRIATAR
                     KE"
     gene            10625..10999
                     /locus_tag="PLE39_09075"
     CDS             10625..10999
                     /locus_tag="PLE39_09075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE68520.1"
                     /translation="MPTYDFICNKCEKRFEVFQTFSEYGKKTVHCTHCNSENVRRRMT
                     KVRIAKSESSRIETATDGLAGMEEIGDDPKALARMMKKMGNEMGEDIPTEFNEVVDRL
                     EAGQTPEEIESAMPVLGDTKNE"
     gene            11117..11662
                     /locus_tag="PLE39_09080"
     CDS             11117..11662
                     /locus_tag="PLE39_09080"
                     /inference="COORDINATES: protein motif:HMM:NF021386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HNE68521.1"
                     /translation="METNPEMLDFVKAMSNPNRLRIIGLLSQNPLSRAEITARLNLSP
                     KEALNHLTFLENVGAVLQTEEIYTLNDDRLAALAREKLAEERPVFIPAPDLDEKSKKV
                     LKAHLNADGSIKQIPAQPKLQAILNYLVGFFEFGTNYTEKEVNTIIRRFHIDTAGLRR
                     DLIEAGLLNRESDGSRYWRVA"
     gene            11659..12213
                     /locus_tag="PLE39_09085"
     CDS             11659..12213
                     /locus_tag="PLE39_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE68522.1"
                     /translation="MSEELVAFFKALADANRLKIVGLLAQQPYSVEELAALLNIKPPT
                     VSHHLAKLAQVGLVSAKADSYYNVYQLDEKALETKSKSLFSQENLAASVANVDADAYD
                     NKVVKDYVRKDGSLKTIPAQKKKLDAILRYVVKAFELEKRYSEKKVNEILAQYHEDTA
                     SLRRELVGAGLMKREGGGGEYWRV"
     gene            complement(12210..12716)
                     /locus_tag="PLE39_09090"
     CDS             complement(12210..12716)
                     /locus_tag="PLE39_09090"
                     /inference="COORDINATES: protein motif:HMM:NF025160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HNE68523.1"
                     /translation="MNLLDEISFVRLLWGVPILLAIHNLEEAPFMEKWSKELGLPIHF
                     AISTRQFIWAVTLLTAAGFAATYLGINTTKQPIGVWAVLEIQMIMFVNAVVPHFAAFL
                     RFQRYNPGVVTALFLNVPFSVYLFQRAMQAGVMSWGVFWVLLVIAPFAAVVLIFTSLQ
                     FGKWVSRN"
     gene            complement(12700..13923)
                     /locus_tag="PLE39_09095"
     CDS             complement(12700..13923)
                     /locus_tag="PLE39_09095"
                     /inference="COORDINATES: protein motif:HMM:NF017019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HNE68524.1"
                     /translation="MKIRSITYFCNPKNPFDEKILRSAGDFLSRAKSAFEAGGYEVQT
                     VRLATTPFPNLLNGRVGDLPAIAQGLANLLPQIGIAYASLGPALIEFPESYAVIPDSI
                     AASENIFFGGVMADRKNGISMQAVKACAEVIQRAATIEANGFANLRFAALANVYAGAP
                     FFPAAYHDADKPAFAIAVESADVAVDAFAGQSDFSEGRSALVAEIQRHGQSIARIAES
                     ELSSTRFLGIDFSLAPFPDDAHSLGNAVEKMGVPKIGLHGSLAAAAILTEAIERADFP
                     HTGFSGFMQPVLEDSVLARRAAEGTLTIKDALLYSAVCGTGLDTVPLPGDVTADEMSP
                     LLLDLCALALRLDKPLTARLMPVPGKRAGDVTEFDFGFFANSKVMRLDSAPLTPPFSA
                     SQNIHLNARKHESAG"
     gene            complement(13935..14720)
                     /locus_tag="PLE39_09100"
     CDS             complement(13935..14720)
                     /locus_tag="PLE39_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNE68525.1"
                     /translation="MITSNHNPRIKFVRALQGRARERRDAGAFVADGVRLVEEAANSN
                     WIFQFALYDETLNERGKILVENLKSRGVEVEEVSASVMKSLSETETPQGILAVLEFTP
                     LPVVPHFDFILIPDQIRDPGNLGTLLRTAAATGVQAVLIPPETTDAFSPKVLRSGMGA
                     HFRLPIIPKSWEEIEQVVEASSLQVLIADMGGNSCWETDLCQSLALIVGSEAEGASDA
                     ARKLADQQISIPMSGAVESLNAGVAGSILMFEVVRQRVVNNKQ"
     gene            complement(14751..15314)
                     /locus_tag="PLE39_09105"
     CDS             complement(14751..15314)
                     /locus_tag="PLE39_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNE68526.1"
                     /translation="MNTILVERILPTGQVLQIVQGDITIETVDAIVNAANEHLAHGGG
                     VAWAIVRRGGEVIQQESDEWIKQHGPVSHAHPAWTLGGALPAKYVIHAVGPVWGDGGE
                     DGKLADAIHGSLQVADELKCKSMSVPAISTGIFGFPKDRAARIIFSAIEKYFAAVKTS
                     TVQTIRLVLFDHATIQVFLDAWNTANP"
     gene            complement(15375..15716)
                     /gene="rplT"
                     /locus_tag="PLE39_09110"
     CDS             complement(15375..15716)
                     /gene="rplT"
                     /locus_tag="PLE39_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002507379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNE68527.1"
                     /translation="MRVKGGPQGHLRHKKVLKITKGQRGSKSTLFKRANEAMLKSLWY
                     ATRDRRVRKRDLRKLWIARINAAARLNGTTYSKLVSALKKANIDLNRKMLADIAVRNP
                     QAFAAVVAKTK"
     gene            complement(15821..16054)
                     /locus_tag="PLE39_09115"
     CDS             complement(15821..16054)
                     /locus_tag="PLE39_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HNE68528.1"
                     /translation="MPRKAKKSGKIKLKTHKATSKRFRLTGSGTLVRTKGGKSHLRRR
                     TSDRTKALLSEMVVVKGRKFIKRIKRLAPHMEG"
     gene            complement(16161..16712)
                     /gene="infC"
                     /locus_tag="PLE39_09120"
     CDS             complement(16161..16712)
                     /gene="infC"
                     /locus_tag="PLE39_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNE68529.1"
                     /translation="MNEGIRVAEVRLIGPDGGNVGVVPIRQALQIARDAEMDLVEVSP
                     GATPPVCRVMDFGKFIYEKAKKEREARKSQTKIEVKEIRLRPKTNGAHRGFKVDDARR
                     WLGQGHKVRVTVKFRGREMDYPEIALEDLREIVQDLVDVAVVEVPPQMEGRTMLVVLA
                     PAKGAGKKKEKSEQAEVKTEAEA"
     gene            complement(16860..17876)
                     /locus_tag="PLE39_09125"
     CDS             complement(16860..17876)
                     /locus_tag="PLE39_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68530.1"
                     /translation="MRVKKFLFTILVIAILSACGGSATEVAGPTAVPTHTAVPTSTMI
                     PTPSTPLAILVLPADMDKASSDLYQKTVYDLAQQSGFRFQVRNTLTPVDLADPTLKVV
                     IALPPDPGIASFAPTAPNVQFLAVNIPNLIAGGNLSVLAGDSQVELPAFMAGYVGAML
                     SDEYHIGMVYPQGDADAQRAAVAFKNGMTYYCGLCKTFYISEIVYPTFVEVPVNEDPA
                     RYGGYANVLINDRKVYALYIYPSLANADLLSYIGTQGVMLIGTSMPETRPGGWVMTIR
                     PDTIKAIQTAWPNLIAGQGGQNVQSPLGIADVDPSLLTPGKLRLAQQTLESLLAGRIL
                     TSNP"
     gene            complement(18012..18395)
                     /locus_tag="PLE39_09130"
     CDS             complement(18012..18395)
                     /locus_tag="PLE39_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNE68531.1"
                     /translation="MTIYIQPHQFRRMARRWAEQPQQRALGVNIREQDDAYVLSALVP
                     GLKAEDLNIQILENVVSIEGAYQESDGEYLLNELPSGSFRRALRLPTEVEADKAEAKI
                     ADGVLTLNLPKAETARPKKINISLN"
     gene            18542..18997
                     /locus_tag="PLE39_09135"
     CDS             18542..18997
                     /locus_tag="PLE39_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68532.1"
                     /translation="MKTYGTLLFLSSLLFLAGCQPESSPVTNTALPAMSFSQTPPPAQ
                     EEGIEMFNEGTPVDPQAQKMIQLAKESLVKKFKLNEDQVHVASIQAMDWPDAGIGCPQ
                     AGMVYAEVITPGYQILFEANGQSYSYHTDTVNKVLLCRIHPVDDIFLTP"
     gene            complement(19060..21312)
                     /locus_tag="PLE39_09140"
     CDS             complement(19060..21312)
                     /locus_tag="PLE39_09140"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HNE68533.1"
                     /translation="MKAKWFSTILILALLVMSLVPAAGAAPSLEDEGPIFAGKGDDNL
                     SHPMGDAQAALKAKAMEAMLQGKAKGKTHEVFRGQFAELALEKNDRVFVIVAEFGDAI
                     HPNYGGEPGPLHNQIPEPDRTVDNTTIWQADFDKTHYEDMYFNQMVDYYAKQSSGRYT
                     INGDVIDWVKVPYNEARYGSNFCGSTTCSTVWYLIRDAINQWVVDQKAAGMSNQEIHD
                     YLATFDVWDRYDADGDGNFDEGDGYIDHFQIVHAGVGEETGGGAQGEQAIWSHRWYTS
                     YQGIGVWGANGLGGMEFGGNGTFANEGGPNAVGIWVGDYTMQPENGGLGVFAHEYAHD
                     LGLPDQYDTAGGDNSTGFWTIMSSGSYLGDGSTDIGSRPGDFNAWEKFQLGWLDYTVA
                     KEGAKSEIVLGPAETTTQHAQALFVTLPDKEVTVNLATPYSGSYFYYSGAGNDLDNSM
                     TRSVTLPSGAASLTAQVNFQIELDWDYAYVTVNGSPVATNLSTNDNPNGQNFGEGITG
                     DTGGNWVALTADLSDYAGQTVDIGFNYWSDGAVAELGFMVDDVAITGQPLDDAESNFG
                     WNFNGFSATTGTETSYYFNAYVAEYRQYRTYDAGLQTGPYNFGFGNTMPDWVEHYPYQ
                     DGLLISYWDSSFTDNNTSEHPGGGLILPIDSHPNALIRPDGNPWRSRIQAYDSTFSIT
                     RTDGFTLHLNGEPSTIPSQPGVRLFNDALQYWNWTTPLAGVINPHTGTMIRIKGINAQ
                     GFMQVQVKAP"
     gene            21617..22072
                     /locus_tag="PLE39_09145"
     CDS             21617..22072
                     /locus_tag="PLE39_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68534.1"
                     /translation="MKKIINILIFSGLLFLAACGGAAAPAAPNSENPPLATPPPVDSA
                     NEPEQPAAESPIEQNMVNLSLENLSRKLNIGVEQIRVTKVTKVVWRDASLGCPKPGVD
                     YLRVETPGFSITLEADGKSYELHTDESKRVTICNNAQPKGTTPRWKSLI"
     gene            complement(22145..24013)
                     /locus_tag="PLE39_09150"
     CDS             complement(22145..24013)
                     /locus_tag="PLE39_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68535.1"
                     /translation="MKRKLFSIIAVLVMMLGLIPATALAAPAPQEGFGGGGVTMFTQR
                     VPINVVFLGYDKNAINQKAIRDLLPASYTPIVRYPAFYGLEGRNLGLRFDFNYTVSFV
                     NQKTTDRFFKFLTQGSTVAGPTFYQNAYNDMENNVLDVADEVLYADAPSVESWLNQNL
                     NVNKKGYTIVFINWYGRPDFKFHVYTKTDVADPDTGYNFGEIRSSRKTIAWGGSSSRL
                     WFYDLSAGPEAWTGNYDVDNPDLDGNGVEDYRMPPIWEYSANGYRDPSALSTDLGLVA
                     RFVGINLLFTTSPLYDPLASAPEVNGSKIVHINMMEDDPASQGTDWINPGLAQQRFSE
                     FQPYYNWQVNLTDVNPIDANAEKSFRIWAELDGSDDCWNDWGTPYAQLFCYFDINRNA
                     YIPEYTDADYVAAFHEFNTTADNLGNQYGLLGFADDNWIDGTPSYVFAFDTDDYRAGG
                     YGFTITTIHEGGHHFGLSHPHDGYDSELGLDYGASDDFYFANSGDESNTIMHYMDLSD
                     EFGQFDRDNMYRIEMAGYLMWSNSLLNQILANPNAASVRGYISQAKNDGNRAVARFNQ
                     WNYLEAVSYARHAYEQLSLAAIQLGIPTPSSQYEPAMANPGMPVPHEGDPIRFPDN"
     gene            24288..24983
                     /locus_tag="PLE39_09155"
     CDS             24288..24983
                     /locus_tag="PLE39_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68536.1"
                     /translation="MSKRTIIPIFLLICIAGGLYYYFIGRRGSARTAEVYNWLRDSTS
                     RPELMMTTGTTCGDAPFIFPTNGLIGFIWDDSFRPGHRHSGIDIFAGTDVGITPIVTA
                     YPGYLTRKSDWKSSVIIRVPEDPLQPGRQIWVYYTHMADPNGNSYISPEFPTGTTEVF
                     VEAGTFLGYQGNYSGDPNNPVGVHLHVSVVKDDGFGNFTNELDINNTYDPSPYFGLPL
                     NANENTDTIPVCN"
     gene            24997..25596
                     /locus_tag="PLE39_09160"
     CDS             24997..25596
                     /locus_tag="PLE39_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE68537.1"
                     /translation="MDLRNKVVLITGAGKGAGAALARAFAEQGALVALNDISPINVEQ
                     VAAEITAKGGKAKAYVEDIAKKVGVQYVIKGVEDDFGGMDILVNHAVVKPHTTLLDVD
                     EWDWHRTLDVNLTGAFLTIQSAGRIMREKGHGAILNIVAEAGIRPESEAGAYPASMAG
                     LVALSHQADRELSPLGIRVYAVENSADVVKNVIKILEAQ"
     gene            25593..26054
                     /locus_tag="PLE39_09165"
     CDS             25593..26054
                     /locus_tag="PLE39_09165"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE68538.1"
                     /translation="MKELYEYREKMIARLAQAAREFCAACEAFENPLTQVEGEWNLHQ
                     IASHVRDVEKLVYGDRIRKTLEMENPAFQSFDADSWMETNYNQNESLQAILSEFSSNV
                     DALCQTLKNLPQSAWSRESSHETMGGELALQLWVERGLAHIEEHLQTLKKA"
     gene            26105..26764
                     /locus_tag="PLE39_09170"
     CDS             26105..26764
                     /locus_tag="PLE39_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE68539.1"
                     /translation="MKKQRIILVDDHEVVRLGLKSLLERHPHFDVVGEAGSAREALEQ
                     VEALKPDVVVMDIRLPGTSGIDACEQIVSQFPNTKVLMLTSYAEDEMLFSAIRAGASG
                     YVLKQIGSEDLIKAIESVGRGEALLDPAVTQRVFQEVRRAVKEEEASAFAHLSQQEKH
                     VLLLVSEGKTNREIAKNLFLGEGTVRNYVSSILSKLSVNNRAEAAAYAVQHSLREYIT
                     L"
     gene            26906..27472
                     /locus_tag="PLE39_09175"
     CDS             26906..27472
                     /locus_tag="PLE39_09175"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNE68540.1"
                     /translation="MFPFLKLTDGTITLRPFEFGDEKDLRDAVQESLPELQPWMSWAT
                     EKYNLETARNFIAITRGQWSSGSLYAFAIADAVTDELVGGCSLSHIHPVYYFCNLGYW
                     VRSSRHGEGIAGAAAKLAAHFAFERVGLIRTEIVIAVGNEPSRRVAEKIGAHYEGILL
                     NRMVVGQAIHDAHMYSLLPSDFGLTAKR"
     gene            27579..28250
                     /locus_tag="PLE39_09180"
     CDS             27579..28250
                     /locus_tag="PLE39_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNE68541.1"
                     /translation="MSKSSSPLLFLGKADDPDCARALEFCQSHFEHVTFCLGKWGDPL
                     PETARNWEGDIIISYLSRWVVPADLLNRAKIAAINFHPASPEYPGIGCNNFALYENAK
                     EYGVTCHHMASKVDTGRIVAVKRFPIFPEDDVDALLKRTYENQIALFFEIANLLAEGK
                     ELPTSAETWTRPPFSRKQFNELFIITPDMNEDEVNRRIRAISYGPWQPYMEINGHRFE
                     YKPEK"
BASE COUNT         7123 a         7596 c         6787 g         6696 t
ORIGIN      
        1 caagcagttc atgtttacca aacaaaatgc ccgcatgagg accgaagaat ttataagcag
       61 atgaaacaag aaaatcgcaa tcgagccttt gcacatcgat ggggccgtgc ggtgcgtatt
      121 gcacggcatc cacatacacc tgtgtgccgg catcgtgagc catttggatg atcttctgaa
      181 cagggttgat cgtgccaagg gaattcgacg catacccaac cgccagcaga cggggttttc
      241 gttcaagcgc cgcctgaagc tggtccagtt tcaatgtgcc atcttctacg tcgaaatcca
      301 cccaattgac cttcaccccg cggtcttgag ccgccagcac ccagggcgaa acgttggcat
      361 catggtcgag gcgggtgacg acgatctctt ccccctcttt ccagtcgcgc gaaatggagc
      421 ggctgaggtg cagggtcagg gtggtcatgt tgttcccaaa gacgatctct tcgggcgaag
      481 aggcattgta gaaatcggcc atggcgcggt gggcttcatc cagcacggca tcggaggcga
      541 tgctggtggc aaaggccccc tcgtgatttg cattgctttc tattaaatac ctgttgatgc
      601 ggtcaaggga gtgttttgcg atctgagtgc cgccggggtt gtcgaagaac gtggcggggc
      661 ggtctaatgc ggggaattgt ttgcggatgg cagagacatc aatcgtcatg ggaagactcc
      721 aaaattgaag tgaaaactgg cggttattat agtccatccc tgtgtgagaa acctttttgc
      781 cgcctcagcg tagatttgca tggctgtgac ggcgtaattg ccgtaattga cagttatgca
      841 agggaaatct tatccgggct attttttgtt atgataacga caatggttga ttgaacatga
      901 tcacaaatcc cgtcaccagc gagacctcac taaaaagccg ccccggcttg cgccgtgcat
      961 tgcttggcgt ttggtctgtg gttgttctct acattgtgta tgttctgggg gaggcgattg
     1021 cgccgcagtt cggcaatgcc atgaaggcat ccatggcgca aaacctgctt gggatctcct
     1081 ccctgctgca aatcggtttt tattacattg tggatattct gctggtgctc ggtttttcgg
     1141 tcattggggt gatgatcgtc atgcacagtt ccgatgactg gtttgcgatt ttcacctcga
     1201 tctttctgat caccttcggg gtacgcgtta ctgacctggc aaatgcaatc gcattgcagc
     1261 cgggcacgga atttgcaggc ggattgctgc tggccatggg tgatattggc attgtcctgt
     1321 tcacaatgct cttcccggat ggaaaattca caccccgttg gctgcgtttt ttggccattc
     1381 cgctcgtggt cacaatgctg ggaatctatc tctttcccag tgcgcctttc ttttgggaca
     1441 aactcgggca gggctacctg ttgatcacca cctcctggta tctgatcggt ttcttcaccc
     1501 tgctcaaccg ctatttccgc aaggcaaccc tcgcccataa acaacagatc cgctgggtat
     1561 tcatcggcac tttggggcca tttctatggt tcatcatctt tcaggtgggt ctatcctttc
     1621 tgccgaagcc cgcagaatct tccctgacca tgacggctat tctgcttgtt tcccgcctgc
     1681 tgagcatttt cctttttttg gtacttccgc tctccatttc catttccatt gcacagacaa
     1741 aactcttcaa tattgacctc atcatcaacc gctcccttgt ctatggcagc atgacactcg
     1801 cgctggggct gaccttcgct gtgatgcttg ggttgatctc gctggtcttc aagaattttc
     1861 atcaaggaga tcaatccctt gtggcggcca cgatcttctc cgtttcagca ggggcattgt
     1921 ttcagccagc ccgaaaaaaa ctgcagcgct ttgtagatcg cttcttctat cacatcgaga
     1981 tcgattatca gaaaacccca tcagatatca ccctttctcc cgattcttcc tccacaacgc
     2041 cgagattttc ttcatacaat gacctgcgcc tgatcgggcg gggcggcatg gcagaagtct
     2101 atcgcgcaac cagccagacc aagaataaac ccgtagccat taaagtgctg cgttccgccc
     2161 tggcctccga cgattcattc cgcaagcgtt tcatgcgcga ggcggagatc atcgccagat
     2221 tgcaacaccc aaacatcgtc aacatcatgg actttggaga tgagaacggc gtctatcaca
     2281 tcgtcatgga gcatctctcc gggccagacc tcaacaacct cattaaacag gaaggccgca
     2341 tcaccctccc caagaccatc tccattttgc gcggtgtagc cgatgcattg gattacgccc
     2401 accagcatgg tttggttcat cgggatatca aaccctcaaa cgtgatgctg gattcgtcca
     2461 ccattccgcc gcgggtggtg ctgaccgatt tcggcatcgc caagatcgtc gattcgcaga
     2521 cactgatgac aaacaccaac atgcttggca cgcttgatta catcgccccc gaacagatcc
     2581 aagcctccgc cgaagtggat agccgcgccg atatttacgc gctgggcatc atgacttacc
     2641 aaatgctgac cggcaccctg ccgttcgaac aggccagcac cggcgccctc ctgcttgccc
     2701 atctcaatgc cccgcctcca gatgcccgca ccatcgtccg tgacctgcct cgtaatgtcg
     2761 ctcacggcat tcaacgggca atggcaaaga aacccgaaag ccgcccatcc accgcgggtg
     2821 aattcatcac catattggaa aacgcctagc ttttcaccca attacagggg aaatagtcgg
     2881 tgttcattat tttggtctgc gcaaatggcg cagatctttt tctttaatga aaatcagaca
     2941 ttggaactgt atactgaatt taacccacga acaaggagac aacatgttta aaaagattct
     3001 tttggcgacc gacggatcgg agcatgccct gcatgcaacc cagattgccg cggaactggc
     3061 ccgcaatcca accgcaaaag aacttcgtat tgtcgtagcc tacgatgtga ttcctcccta
     3121 ctttggcgag cccaacatgc aatttgccat taatgcccgc atggaagaag cgcagaccat
     3181 cctgcggaaa accattcaaa ccattggcag catcccgtgt gaaatccaca cagaattgat
     3241 cgaaggctca gcggctgaag ccatcattga tgtggcggcc actcgtcaaa gtgatgtcat
     3301 tgtgatgggt tcacggggat tgggaaaact ggctggcttg ttattaggca gcaccagcca
     3361 aaaagttgtt gcatatgcac cctgcccggt gctggttacc cgctagcgcg tttttcagct
     3421 tcggcaaatt cttcgggcgt attcaagttc caaaagcaca agccggaagg atcaaaagcc
     3481 ttcacttctt gcggcaataa ctcacgaacc ttgacctttg aaaaccatga gatgaccttc
     3541 cattgatcgg cctcgatggc agcctcaatg gcaggcaggc aggtctcgcg gcgatacagg
     3601 gcatggaacg gttcatagcc ctcatccgtt ttggcgatga caacatccgc atcttcttcg
     3661 accataagcc tgcgcgcgcc ttcgaagagc acatcgctgg cgaagggcat atcgcaggcc
     3721 accaccgcca cctgtgaaaa agccgccgat gcaactgcgg tgtagagtcc gcccagggca
     3781 ccacgaccag gcttgaggtc cgcgatgagg cgaagaccga gaaaggcata ctcatccgga
     3841 cgattcgtcg ttacaatcac ttcatccgcg atcggtgcaa gccggtcaat aacgcgttgg
     3901 atgagcgggc gccctaaaaa gggtttgagg gccttatcct cacccatgcg cgatgacata
     3961 ccacctgctt gaatcacgac agttannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnntaaca taggctgatt ataatgagtt cactcgcaaa acggctggat ggtttaacgg
     4141 ttgaaggcaa attgtacgaa gcactggaag gcggcgatgt gcgttgcctc gcctgcggac
     4201 atcgttgttt gatccgcgag ggcaggcgtg gaatttgtca ggtacgattt aatagggatg
     4261 gaaaattgtt tgtgccgcat ggttatgtgg cggctctgca aagtgacccc attgagaaaa
     4321 aacctttttc gcatgtgttg cctggcgtca atgcacttac cttcggcatg ttgggttgtg
     4381 actatcactg tgggtattgt caaaactggc tgacctcaca agccctgcgt gacccggcgt
     4441 cagatgtttc ggcgcaattc atccgcgagg tatcgccgca aacaatggtg gaccatgcaa
     4501 aacgcacaaa tgcgtcggtg gtggtctctt cctataatga acctctgatc accagcgagt
     4561 gggcggtgga aatcttcaag gcagccagag ccaatggcct gctgtgtgcc tacgtttcca
     4621 atgggaacaa tacgcccgag gtgatgaaat acatcgcccc gtatctggat gcatacaaga
     4681 tagacttgaa atgcatgagc gataaggggt accgaaaatt gggcggcaca ttgcaacaca
     4741 ccctggatgg catccaacgt gcgcgcgaaa tggggctttg ggtcgaggtg gtgactttgg
     4801 tcgttccggg cttcaacgat tcaaatgaag agctatggga ggcggcacga ttccttgccg
     4861 ggctttcggt ggatattcca tggcacgtaa cggccttcca taaagactat aaaatgaccg
     4921 agccagacaa tacagatggc aatacgctcc tgcgcgccgc tgaaattgga cgggaagcgg
     4981 gattgcgata cgtatatgcg gggaatttgc cgggcaatgt tggggagtat aaagataccc
     5041 attgtccgca gtgcagttat cagcttgtga gaagaagcgg gtatgtcatc caatcgtatc
     5101 agatcacggg ggatggaaaa tgccccaaat gcggggaaaa aatcgccgga atatggccat
     5161 cagacccttc tacagtaggg ttgtatggtg tcggacgccc gttttctgtt tggtagttta
     5221 cttttgttgt ataatttgcc agtcaggagt gtgcaggaaa aaaaaatttc ccatgctcca
     5281 taatccactt atttctctca aggaggagaa acgtgaaacg aaacctttct gttttgcttt
     5341 cgcttgtagt gcttgcaagc atcatgcttg cggcctgcgg cggcggtgcg gcaactgaag
     5401 cccccgcaac tgaagcgcct gctgctacgg aggcccctgc tgccaccgaa gctcccgctg
     5461 ccaccgaagc ccctgcggct tatgagggca tgaaggttga atcccccgac tgcgattacg
     5521 gtggcgaatt caagtccatc gaagcggttg atgaattcac cgtcaagatc acgctctgcg
     5581 tgcccgatcc tgctttcccg tccaagatcg cctttacttc cttcgctatt cagcctgcgg
     5641 aatatctcga atccaccggc ggcaatggcg acctgcttga gaagcccatc ggtaccggcc
     5701 cctacatggt tgaatcctgg gagcgcggca accagttggt tttgaaggcc aaccccaact
     5761 attggggtga attctccggc gccgagaccc tcgtcttcaa gtggggtact gaatctgccc
     5821 agcgcttgct cgaactccag tctggcactg ttgatggtat cgacaatgtc ggtccggatg
     5881 atttcgccac cgttgaaggc gatgccaacc tcgctctctt caaccgcccg gccctcaaca
     5941 tcatgtacat cggcttcaac aacaatcccc aggttgaagg cttcgataac agcaccaatc
     6001 ctctggccaa cgaaaaagtt cgccaggcga tcgccatggg tgttgaccgc cagcgcattg
     6061 tggataactt ctaccccgct ggttctgaag ttgcctctca cttcaccccc tgctccatcc
     6121 cgaacggctg cgtaggcgat gaatggtatt ccttcgaccc cgaagctgcg aaggcccttt
     6181 tggccgaagc tggctatccg gatggtttcg aaaccgttct gaactaccgc gacgttgtcc
     6241 gcggttacct gcctgacccg aacgttgtgg ctcaggacct ccaggctcag ttgaaagaaa
     6301 acctgaacat caccctcaac atcgaagtga tggaatctgg tgctttcctc gctgctgctg
     6361 acgctggtca gttgcagggc ctgtacatgc tgggttgggg tgctgactat cctgatcaga
     6421 ccaacttcct cggctaccac ttcggcgctg gcgcttccaa gcagttcggc gacaagttcg
     6481 atgacatcgt cgaagccctc gataagggtg cccagctcgc ttctgacgct gaacgcgctc
     6541 cgttctacga agccgccaac aacgccatcc gcactcacgt gccgatgatc cccgttgctc
     6601 atggtggttc cgctttggcc ttcaaggcct ctgttgaagg cgctttcgcc agcccgctcg
     6661 gcaacgaaga attctctgtc atgaacaatg gcaccgacac cttcgtttgg atgcagaacg
     6721 ctgaaccgat ctccctctac tgcgctgatg aaaccgatgg tgaatcactc cgcgcttgcg
     6781 aacaggtcac ccagtctctg cttgcttacg aaaccggcgg caccgcagtt gaacccgctt
     6841 tggctgagtc ctacgatgtc aacgctgacc tgaccgagtg gaccttccac ctgcgccagg
     6901 gcgtcctctt ccacgatggt tcctccctcg atgcgaacga cgttgtgacc tccctctcca
     6961 ttcagtggga tgcggccaac ccgctccaca ccggcaacac cggtgcgttc tcctactgga
     7021 gcggcctctt cggcaccttc ctcaacgctc cccccgccca gtaatctgaa tcaaggcaag
     7081 tctattagac ccccggcaga aatgccgggg gtctccccaa attatcatgg tcacttatat
     7141 ccttcgacgt ctcgttctta gcataccggt attatttggc attctgtttg ccacatttgc
     7201 gcttgcgcgc cttattccgg gcgatccctg ccgggcaatg ttgggcgaga aagccaccaa
     7261 ggtggtttgt gatgagttca atcaccgcca tggcttggac aggccgatcc tggtccaatt
     7321 cgggatttac gtgaacgaga tcgctcacgg cgatttcgga aattccttcc gctattccaa
     7381 acccattacc gaattgctgg ccgaacgtct gccgatcact ttcgaattga gcatcgcagc
     7441 gcttgccttc agtgtcatca tcggcattcc actgggcgtc attgcagcgg tccgtcacaa
     7501 ttcatgggct gatgtggcaa ccatgttatg ggccaatgtc ggcgtatcca tgccggtctt
     7561 ttggttgggg ttgatgcttg cctatatctt ctcgcttacc ttgaaggaca ccattttcca
     7621 gcttccgcct tcaggtcgtt tgagcgctgg tttgatcaca actccatttt ttgaagtatg
     7681 ggggtggcaa ctgcaagaag gtactttttc attctccctt gcggatttca tcagccgcat
     7741 gaatgttttt aattcaatcc tttcaggaaa ttgggaagtt ctcaaagacg cgtcgatcca
     7801 cttgattttg cccgccttcg ctttgagcac gatcccgatg gctttgatcg cccgtatgac
     7861 ccgttcctcc atgctcgaag tgcttggtca ggactatatt cgtactgccc gcgcaaaagg
     7921 tctggtgaga cgctcggtca tcttgaaaca cgcatttcgc aatgccttgc tgccactggc
     7981 cacggtcatc ggcttgtctt tgggcggctt gttgggcggc gctgtgttga ccgaaaccgt
     8041 tttcaattta tcgggcgtcg gtagaattct atatgatgga attacagccc gtgattacgg
     8101 cattgtgcag ggatttacag tcgtcattgc aattttcttc gttattctaa atttgatcgt
     8161 ggatgtttca tatgcttatc tcgatccccg catccgtctg gactaggaac tgaccatgac
     8221 aacccaatcc gtctccccga aaaaattgga cgctgaagca ctatcgcttg attcggtcag
     8281 tctctggcag atcacccgcc gtagattatt ccgccgtaaa tcatccattg taggcatggt
     8341 cattcttgga ctgttgattt tcatcgcact gaccgcccaa tggcttgccc cgtacgaccc
     8401 gaccctgtcc atgcttgatg cagacccgcc tcaaaaagtg gaaaagcgcg cctcaccatg
     8461 catccatgcg ttgggatgcc ctgccgatca gcctcagcac atcatgggca cagacggcaa
     8521 cctgcgcgat gaattcagcc gaatgctgta tggcgcccgt ctgagcctga tgattggttt
     8581 ctccacagtg acctttgcca tcatcatcgg caccgttctc ggtgcgctgg gcggctattt
     8641 tggcggttgg atcgataaca tcatcatgcg tgtgatggac gtgctgctgg catttccttc
     8701 actgcttttg gccattgcca tcgtcaccgt gatcgggcct gggttgatta acgccttgct
     8761 ggccatcggt atcgtatcca tccctgcgta tgcccgggtg gtgcgcggaa gcgtactttc
     8821 cgtccgtgaa atggattatg tgtcggctac gcgtgcgctt ggcggcaaca cctttgagat
     8881 cctcttcaag aggatcctgc cgaatgcgct gaccccgttg atcgtgcagg gcacattggg
     8941 catcgcctcc gccattttgg acgctgctgc cctgtccttc ctcgggttgg gtgctcaacc
     9001 ccccacacca gaatggggtt ccatgctcgg cgcggaacgc aatcaggtgt tcaccgcccc
     9061 tcaccttgtg ttttaccccg gtctggcgat catgttgacc gtgttggcct tcaacctgct
     9121 gggtgatggt ctgcgcgatg cgctcgaccc acggcttgcc cacacaagct aaccctcatt
     9181 tgaaaatcaa aacgcggcca gtatttggcc gcgttttttg tttaagcaag tccttctctt
     9241 ttaactcttc gtgcatggaa ttttagtttc ataacgaaaa agaacccaga aggcagcagc
     9301 atctaggaaa acatcccaaa aatcccaagt gctccaaaaa taacgcagac tgcagccgca
     9361 ccgacaatga tttcgcgcat ttcacgacgg tcttgcaaat gaaaatcggg cggggttccc
     9421 acaaccatcc aaaacggacc ggcaaaccag gtaaagattg caccgccaaa caagccccct
     9481 acatgccccc aattatcgat ccctggagca aggccaatga ataaattgac tgcaataatg
     9541 aaaccagcat tactgatcgc ctgtcgggcc tgcccgccaa acagctttcg gttttggtaa
     9601 aaaaagatga cctctgccgc aatcaaacca aagaccgcag ttgatgcacc aattgaatat
     9661 ccattttcac cggtgagtaa aaaagaaaat acatttccag caaaggcccc aaggaaatac
     9721 aacaaggcaa acctgccatg accaaaatgc cgctctaaaa acgacccaat ggataacagc
     9781 gcatacatat tgaaaaagat atgagccatc gaaccatgca aaaagaccgg ggtgataaaa
     9841 cgccataact cacccatgcg gatcgcttca ttaaacctgg ccccgtacac ttccagccag
     9901 tcaatttcgt aaaccgcata cccaaagaca gccacactca acaattgcaa aacatacacc
     9961 agcacagtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct ctttacgagc
    10081 ggttgcaata cgcaaagaca gcgaatctgc tgtttcggtc tttcgctccc gcagccgccg
    10141 ctcaagttca tcttcgcctt cacaggtaat aaagatcaac aacgcctcgg gggcgagttt
    10201 tcgcactgtt tcagcacctt gcacatcgat gcgcatcact acatcctgcc cgctggcgag
    10261 cgcctcacgc acctcccact tgggaatacc cttgtaatca ccatacacga tcgcatattc
    10321 gatcaattcg ttttcatcga tcatgcgcgc aaattcatcc ttcgaaacaa accaataatc
    10381 cctgccattt gtttcatttt cgcgcttatc gcgtgtggtg gccgtgacta caaaatgaaa
    10441 tggaaaaccg cgctctttca tgcgctgtac gacagaatcc ttgcccacac cagaggggcc
    10501 ggagatgaca atgaggagtg gattgggttt gcggagttca aatgaagtag gcatgggggt
    10561 attgtaccaa agagatatcc gcctctcccc agaccttggg cctgattcgc agtaaaatat
    10621 attcatgccc acttatgatt ttatctgcaa caaatgcgaa aaacgatttg aagtctttca
    10681 aaccttcagc gagtacggaa aaaaaaccgt ccactgcacg cactgcaaca gcgaaaacgt
    10741 ccgccgccgt atgaccaaag tgcggatcgc caaatcggaa tccagccgaa tcgaaaccgc
    10801 cactgatggc ctggcaggca tggaagagat cggagacgac cccaaggctc ttgcacgtat
    10861 gatgaaaaaa atgggcaacg aaatgggtga ggacattcca actgaattca acgaagtggt
    10921 ggacaggctg gaggcaggcc agacaccgga agagatcgaa tcggccatgc cagttctggg
    10981 cgatacaaag aacgagtaga aaccgccctg tggaaaaatt ccacagggcg gttttctttt
    11041 taccagttga caaaaccaaa agccttcgct aaaattcaat tagatagtca tcaaattaga
    11101 tttacggata taaaaaatgg aaaccaaccc tgaaatgctt gattttgtaa aagccatgtc
    11161 caacccaaac cggttgcgta tcattggtct tctctcacaa aacccgctct ctcgtgcgga
    11221 gattacggca cgcctcaacc tctcccccaa ggaagcgcta aatcacctca cttttctgga
    11281 aaacgtcggc gcggtgctgc aaactgaaga aatctacaca ttaaacgatg acaggcttgc
    11341 cgctctagct cgtgaaaaac tcgcggaaga acgtcccgtt tttatccctg cccccgacct
    11401 ggacgagaag tcaaaaaagg tgctcaaagc ccacctgaat gccgatggaa gcatcaaaca
    11461 gatccctgcc cagccaaaac tgcaggcgat tctaaattat ctggtcggat tttttgagtt
    11521 tggcacaaac tataccgaaa aagaagtcaa caccatcatc aggcgcttcc acatagatac
    11581 cgctggcctg cgccgtgacc ttattgaagc aggtttactc aatcgtgaaa gtgacggctc
    11641 acgctattgg agagttgcat gagcgaagaa ttggttgcct tcttcaaggc tcttgccgat
    11701 gcaaatcggc tgaagattgt cggcttgctg gcgcaacaac cctacagcgt ggaagaactc
    11761 gccgctctgc taaacatcaa gccgcctacg gtctcacacc accttgcaaa gctcgcacaa
    11821 gtcggccttg tcagcgccaa agccgatagt tactataacg tctatcaact cgatgaaaaa
    11881 gcactggaaa ccaaatcgaa aagcctgttt tcacaggaaa atcttgccgc ttccgttgca
    11941 aacgtggatg ctgatgccta cgacaataaa gtagtcaaag attacgtccg caaagatggc
    12001 agcctcaaga ccatccccgc ccaaaagaaa aaactcgatg ccattctgcg ttacgtggtc
    12061 aaggcatttg aattagagaa acgctattcg gaaaaaaaag tgaacgagat tcttgcccaa
    12121 tatcatgaag atactgccag cctcagacgt gaactcgtcg gcgccgggtt gatgaagcgc
    12181 gaaggcggag gcggagaata ttggcgggtt taatttcggg agacccattt cccaaattga
    12241 agtgaggtaa agatcagcac cacagcagca aagggagcga tcaccagcaa aacccagaaa
    12301 acgccccatg acattacccc cgcctgcatg gcacgttgaa aaagataaac agaaaatgga
    12361 acattcaaaa ataaagccgt gacaacaccg gggttgtatc tctgaaaacg aagaaaagca
    12421 gcaaagtgcg gcacaaccgc attcacaaac atgatcatct gaatttcaag aacagcccat
    12481 actccgatgg gctgttttgt tgtattaatt ccaaggtacg tagctgcaaa acctgccgcg
    12541 gtcaacaaag ttacagccca gataaattga cgggtggata tcgcaaaatg gatcggcagc
    12601 cccaactcct tagaccactt ctccatgaaa ggcgcttctt ccaaattatg aatcgccaga
    12661 agaatgggca caccccacag caatcggaca aatgaaattt catccagcag attcatgctt
    12721 gcgtgcgttc aagtgaatat tctggcttgc tgaaaatgga ggggtcaatg gggcggaatc
    12781 caatctcatc accttgctgt tggcaaagaa cccgaaatca aattcagtga catcccctgc
    12841 ccgtttaccg ggaacaggca tcagccgcgc agtgagcggc ttgtccaatc ggagcgccag
    12901 cgcacacaga tccagcaaca acggcgacat ttcatccgcc gtcacatccc ctggaagcgg
    12961 taccgtatca agccctgtgc cgcacaccgc cgagtaaagc agcgcatcct tgatcgtcaa
    13021 cgtaccttcc gcagcgcggc gagccagcac agaatcttcc aacacgggct gcatgaatcc
    13081 gctgaaacct gtgtgcggaa aatcagcacg ctcgatggct tctgtaagaa tcgcagcggc
    13141 ggctaacgaa ccatgcaaac cgattttcgg cacgcccatt ttctcaaccg cattccccag
    13201 tgaatgcgca tcatcaggaa acggcgcaag ggaaaaatca attccaagaa aacgagtact
    13261 cgaaagttca gattccgcga ttcgggcgat ggattgaccg tgtctctgaa tctccgcgac
    13321 aagagcgctt cgcccttcgc tgaaatctga ttgccccgca aaagcatcca ccgccacatc
    13381 agcggactct accgcaattg cgaacgctgg tttatctgca tcatgatatg cagcagggaa
    13441 aaatggcgcc cctgcataca cattggccaa agcagcaaac cgtaaattgg caaatccatt
    13501 tgcctcgatc gttgcggccc gttgaatcac ctccgcacaa gccttgaccg cctgcattga
    13561 aatgccattt ttccgatctg ccattacacc gccgaaaaaa atgttttcag aagcagcgat
    13621 cgaatcagga atcacggcgt agctttcagg aaattcgatc agcgccgggc caagcgaagc
    13681 ataggcaatt ccaatttgag gcagaagatt cgccaacccc tgcgcgatgg caggcaaatc
    13741 ccccaccctg ccattcaaca aattcggaaa tggtgtcgtc gccaaacgca cagtctgcac
    13801 ttcgtaacca cccgcttcaa aagcagattt cgccctggac aaaaaatctc ctgcgctgcg
    13861 caggatcttt tcatcaaatg gatttttagg attgcaaaaa taagtaatgg atcgaatttt
    13921 catagtctct ctgtttattg tttattgttt actaccctct gccttaccac ttcaaacatc
    13981 aaaatgctcc cagccacacc cgcatttaac gattccaccg cccctgacat tggaatacta
    14041 atttgctgat ccgccaattt tcttgccgcg tcacttgcac cttcggcttc gctccccacg
    14101 atcaacgcca atgactggca cagatctgtt tcccagcatg aattgcctcc catgtctgcg
    14161 atcaaaacct gcaaacttga ggcctcaacc acctgttcaa tttcctccca gcttttggga
    14221 atgatcggca atctaaaatg cgctcccatc cccgatcgga gcaccttcgg cgaaaatgca
    14281 tctgttgttt caggcggaat cagcacagcc tgcacacctg tcgctgctgc agttctcaaa
    14341 agcgtcccaa gattccccgg gtcacggatc tgatctggga tgaggatgaa atcaaagtga
    14401 ggtacaactg gcaatggggt aaattcaaga accgccaaaa taccctgcgg ggtttcagtt
    14461 tcgctcaagg atttcattac gctggctgag acctcttcca cttcaacgcc gcgtgacttc
    14521 aagttttcaa ccagtatttt tccgcgctcg ttcaacgtct catcgtacaa ggcgaattga
    14581 aatatccaat tgctgtttgc cgcctcttcc accagccgca ccccatctgc aacaaatgcg
    14641 cctgcatcgc ggcgttcgcg ggcacgtccc tgcaaggcac ggacaaattt aattctggga
    14701 ttgtggttgg atgtgatcat gtagacagga aaaaataatg gatggctgta ttatggattg
    14761 gccgtgttcc aggcatcaag aaacacctga atagtggcat gatcaaacag aaccaaacga
    14821 atggtttgaa cagtggaggt tttgacagcc gcaaaatatt tttcgatagc agaaaaaatg
    14881 attcgggctg cacggtcctt cggaaaacca aaaataccag tggaaatcgc agggacggac
    14941 atggatttac atttcagttc atcggcaacc tgtaaagaac cgtggatggc atctgccagt
    15001 tttccgtctt cgccgccgtc tccccaaact gggcccacag cgtggatcac gtatttcgca
    15061 ggcagggccc cgcccaaggt ccaggctgga tgcgcatgtg atacgggtcc gtgctgcttg
    15121 atccactcgt cactttcctg ttggatcact tctccgccgc gccgcacgat cgcccaggcc
    15181 acacccccgc catgtgcaag gtgttcattc gccgcattga cgatggcatc cacagtttcg
    15241 atcgtaatat caccctgaac gatctgcaaa acctggcctg tggggagaat ccgctcaacc
    15301 agaatggtat tcattacttg attttacccc gttaaaaaaa gtagagcgag aaaattttct
    15361 cgctctacaa ctttttactt tgttttcgct actactgctg cgaaggcttg cggatttctt
    15421 actgcaatgt cggccagcat cttgcgattg agatcaatgt tcgccttctt gagtgcggaa
    15481 accaacttgc tatatgtggt tccgttcaag cgggccgcgg cgttgatacg ggcgatccac
    15541 aacttgcgca gatcgcgttt gcgcacacgg cgatcacggg tggcgtacca caggcttttg
    15601 agcatggcct cgttcgcacg cttgaacagt gtgctcttcg agccgcgctg gcccttcgtg
    15661 atctttaaaa cttttttgtg acgtagatgc ccttgcgggc ctccttttac gcgcatgtta
    15721 ctttacctcc tgcaaactcg cgggcgtcgg tgcgccagaa gcgtacacca gttgcgcaca
    15781 cccgaaagcc gcaactaaaa aatgacgaca accggaagga ctagccttcc atatggggag
    15841 caaggcgttt gatgcgcttg atgaacttgc ggccttttac gaccaccatc tcgcttaata
    15901 acgccttggt gcggtccgat gtacggcggc gtaagtgact cttaccacct ttggtgcgca
    15961 ccagagtgcc ggagccggtc aaacgaaatc gcttggatgt cgctttgtgg gtcttcagct
    16021 tgatttttcc agactttttt gctttgcgag gcattgctgg gatactcctt tcagacaaaa
    16081 taaaccgcgg gacatatgat tgcgcccgcg gacgatcaac atcttagggg acttgcactc
    16141 ttacaggtat ttcacaactc tcaggcttcc gcttctgtct tcacttcggc ctgttcgctc
    16201 ttttcttttt tctttccagc gcctttggca ggagccaaaa ccacgagcat cgtacggcct
    16261 tccatttgcg ggggaacctc aacaacagct acatcaacaa ggtcctgaac gatctcgcgc
    16321 aaatcttcga gtgcgatctc cgggtaatcc atttcgcggc cacgaaactt tacggtgact
    16381 ctcaccttgt gaccctgtcc caaccagcgg cgggcatcat ccactttgaa gccgcggtga
    16441 gcaccattgg tctttgggcg cagacggatt tcctttacct cgatcttggt ctgagacttg
    16501 cgtgcctcgc gttccttttt cgccttttcg taaatgaact ttccaaaatc cattacgcgg
    16561 caaacaggcg gcgtggcacc gggtgagacc tcaacaaggt ccatctcggc atcgcgggca
    16621 atctgcaagg cctggcgaat cggcacaaca ccaacattac cgccatctgg cccgatcagg
    16681 cgaacttccg cgacgcggat gccttcattc actcgaaatt cattagtact gatatgctgc
    16741 tcctcgtggt ttatcaaaca aatccagcct tcttgcggct ggtttttagc gggcgaataa
    16801 taccatgaag gtatacgagt cgtcaataaa atgtgccgct tttattgaaa aaaccttcat
    16861 tatgggttgc tggtgagaat cctgccagcg agcaggcttt cgagggtttg ttgcgcgagg
    16921 cggagttttc ccggggtgag caggcttggg tccacatctg caattcccag cggcgattga
    16981 acgttttgtc cgccttgtcc ggcgatcagg tttggccagg cagtctggat cgccttgatg
    17041 gtgtctggac gaatggtcat cacccatccg ccggggcgtg tttccggcat ggaggttcca
    17101 atcagcatta ctccctgggt gccaatataa gagaggaggt cggcatttgc cagtgaaggg
    17161 taaatgtaaa gcgcatacac tttgcggtca ttaatgagta catttgcata cccaccataa
    17221 cgggcaggat cttcattaac gggaacttca acaaaggtcg ggtacacaat ttcagaaata
    17281 taaaaggttt tgcacaaccc gcaataatag gtcatgccat ttttgaatgc aacggcagca
    17341 cgctgggcgt cggcatcgcc ttgcggatat accattccaa tgtggtattc atctgaaagc
    17401 attgcaccga cataaccagc cataaatgct ggcagttcca cctgtgagtc tccggcgagg
    17461 acacttaaat tgccccccgc tataaggttg ggaatattca ccgcaagaaa ctgaacattt
    17521 ggagctgtgg gagcaaaaga ggcaatgcca gggtcgggag gcaatgcgat gaccaccttc
    17581 aaagtggggt ccgcaagatc aaccggggta agagtgttgc gcacttgaaa acgaaacccg
    17641 gattgttgtg ccagatcgta aaccgttttt tgatacagat ccgaagaggc tttatccata
    17701 tctgcgggca ggacgagaat tgccagcggc gtggatgggg tcgggatcat ggttgaagtt
    17761 ggaactgccg tatgggtggg cacagcggtg ggaccagcaa cctccgttgc agagcctccg
    17821 caagcactca atatcgcaat aacgaggatc gtgaagagga attttttaac acgcacaggg
    17881 gaactccaga aattaagaat gcacgaatta tactgtttta ttttacgaaa gcagatcggc
    17941 agaaactcac caaggttgtt aaaaataaaa gagacgcaca caaggtgcgt ctccaatttg
    18001 gaacgtctac tttaattgag agagatattg attttcttcg ggcgggctgt ttcggccttg
    18061 ggcaggttca aggtcaacac accgtccgcg atctttgcct cagccttgtc tgcttccact
    18121 tctgtgggca gtcgtaacgc acggcggaag gatccgctgg gcaattcgtt caacaggtac
    18181 tcgccatcag attcctgata cgcgccttcg atgctgacca cattctcaag gatctggata
    18241 ttgagatctt cagctttcaa accaggcaca agcgccgaaa gtacataggc atcatcctgt
    18301 tcgcggatgt tcacgccaag ggcgcgctgt tgaggctgtt cggcccagcg acgggccata
    18361 cggcgaaatt ggtggggttg aatataaatg gtcatgtttt tgttctcctt tacgaataat
    18421 tgaattgaac ctattttagt gatggtgcat aaaaaaaatg tcgatgaaag atagaaattg
    18481 tgttagaaat gagtgaactt tttatttaca aaaaacgtcc taaaaatacc tggagctaac
    18541 tttgaaaaca tacggaaccc tactcttttt aagtagtctg ttgtttctgg caggctgcca
    18601 acccgagtca tcccctgtaa caaacactgc attgcccgca atgagttttt cgcaaacccc
    18661 tccacccgcg caggaagaag gcattgaaat gtttaatgaa ggtacccctg tagatcctca
    18721 ggcgcaaaaa atgatacaac ttgccaagga aagtctggta aaaaaattca agctcaacga
    18781 agatcaagtt catgtagcgt caatacaggc catggattgg ccagacgcag gcattggatg
    18841 tccgcaggca ggtatggtgt atgctgaagt gatcactcca ggctatcaaa tcctgtttga
    18901 agcaaatgga caatcctact cctaccacac agatacagtg aataaagttc ttttatgccg
    18961 tattcacccg gtagatgata tattccttac tccttaataa aaacttccgc cacagacaat
    19021 ctgtggcgga agcaatcaaa caaatttaca tcattaggtt tacggggctt tcacctgtac
    19081 ctgcatgaaa ccctgtgcat taataccctt gatgcgaatc atcgtgccgg tatgcggatt
    19141 tatgacacca gccaacggtg tagtccaatt ccaatattga agggcatcat tgaacaagcg
    19201 gacgcccggc tggcttggaa ttgtagaagg ttctccatta agatggaggg taaaaccatc
    19261 tgttctggtg atgctaaatg tagaatcgta cgcctgaata cgagaacgcc acggatttcc
    19321 atctgggcga atcaatgcat ttggatgcga gtcgattggc agaatcaaac cgccacccgg
    19381 atgttcactg gtattgttat cagtgaaaga cgagtcccaa taactgatca acagaccatc
    19441 ctgatagggg taatgctcga cccagtctgg catggtattt ccaaaaccga aattataggg
    19501 accagtctga agcccggcgt catatgtgcg gtattggcgg tattcagcaa cgtaggcgtt
    19561 gaagtaataa ctggtttcag tacctgtggt cgcgctaaac ccattaaagt tccagccaaa
    19621 attgctttcc gcatcatcga gtggttggcc ggtaatggca acatcatcca ccatgaaacc
    19681 aagttcagct accgcaccat cactccagta attgaaaccg atatcaacgg tctgaccagc
    19741 ataatctgaa aggtcagctg tcagggctac ccaattaccg cctgtatcac cagtaatgcc
    19801 ctcgccaaag ttttggccgt tcgggttgtc attggtggac agattagtgg caacagggct
    19861 gccgtttact gtcacgtagg cataatccca atcaagttcg atctggaaat tgacttgagc
    19921 ggtaagagag gctgccccac tgggaagcgt gacactgcgg gtcatgctat tatccaggtc
    19981 attacctgcg cctgagtaat agaagtatga accggaatac ggggtggcaa gattgacagt
    20041 cacttccttg tcaggtaatg tcacaaaaag tgcctgtgca tgctgcgtgg tggtttctgc
    20101 aggtccaagg acgatttcgc tctttgcgcc ttccttggct accgtataat ccaaccaacc
    20161 gagctggaat ttttcccaag cattgaagtc accagggcgg gaaccgatat ccgttgaacc
    20221 atcaccaaga taagaaccgc tggacatgat tgtccagaag ccagtagagt tgtcgccgcc
    20281 agctgtgtca tattgatctg gcaggccaag atcgtgggca tattcatgag cgaaaacgcc
    20341 caagccacca ttttctggct gcatggtgta atcaccaacc catataccaa ctgcgttcgg
    20401 gccgccttca ttggcaaagg ttccattacc accaaattcc ataccgccca agccattggc
    20461 accccaaaca ccaattccct gatacgatgt ataccaacgg tgtgaccaga tggcctgttc
    20521 gccctgcgca ccgccgccag tttcctcgcc tacgccagcg tggacaatct ggaagtggtc
    20581 aatgtaacca tcgccttcat caaaattacc atcgccatct gcatcataac gatcccacac
    20641 atcaaatgtt gcaagatagt catggatttc ctgatttgac atgcctgcag ctttttgatc
    20701 tacaacccat tgattaatgg catctcggat caaataccaa accgtggaac aggtggtgct
    20761 tccgcaaaaa ttcgaaccat aacgagcctc gttataagga accttcaccc aatcaatgac
    20821 atcgccattg atcgtgtaac ggccggaaga ctgtttggca tagtagtcga ccatttgatt
    20881 gaaatacata tcttcgtaat gagttttatc aaaatccgcc tgccagatcg tggtgttatc
    20941 aaccgtgcga tcaggttccg gaatctgatt atgcaaagga ccaggctcac caccataatt
    21001 gggatgaatg gcatcaccga actcggcaac aattacaaaa acacggtcat tcttttccaa
    21061 agcaagttca gcaaactggc cacggaacac ttcatgggtc tttcctttgg cttttccctg
    21121 aagcatggct tccattgcct tggctttcaa tgccgcctgc gcatccccca tggggtgaga
    21181 gagattgtca tcccccttac ctgcaaagat cgggccttcg tcttccaaag acggtgccgc
    21241 accagcagct ggcaccagac tcataacaag cagtgctaaa atcagaatcg tactgaacca
    21301 ttttgctttc ataaatcatt ctcctcacga aaatttgatg atcttacttg caaacaggta
    21361 cttccaattt acttatgctc aatttttgtt gtgaacctcc ttttccttca ggcaggataa
    21421 tcctcaccaa ccctactaaa atcccacact aaaaggattt agaaatcgaa ttattttcga
    21481 cacaaatgca attatcacaa acaaaaggta ttaatgcaag attaatttaa agattaaacc
    21541 cgggcgacca gccttccctt gtccgcagtg aactttcact tccgaaaaac gtctataaca
    21601 ttgaaacgga ggtcgtatga aaaaaatcat aaatatccta atattcagtg gactgttatt
    21661 cctggcagcc tgtggtggcg cggcagcacc agctgccccc aactctgaaa atccgcctct
    21721 tgccaccccg ccgcctgttg attcggccaa tgagcctgaa caacccgccg cagaatctcc
    21781 gatcgagcaa aacatggtca acctcagcct cgaaaatcta agccgcaaac tcaacattgg
    21841 cgtggaacag atacgcgtta ccaaggtgac aaaagtggtg tggcgcgatg ccagcttggg
    21901 atgccccaaa ccaggtgtgg attatcttcg cgttgagacc ccgggattct ccattaccct
    21961 cgaagctgat ggaaaatcct acgagcttca tactgacgaa tccaaacgag ttaccatatg
    22021 taataacgcc caacccaaag gcaccacccc tcgctggaaa agcctgatct gacccaaaaa
    22081 acaaagcgcc ccatgtggac atcacatggg gcgctcttta ctttgtttta ctatttacaa
    22141 aagactagtt atccgggaag cggatcggat cgccttcgtg aggaacaggc attccgggat
    22201 tcgccattgc aggttcgtac tgagaagacg gggtgggaat accgagctga atggcggcca
    22261 gcgaaagctg ctcataagcg tggcgggcat agctcacagc ttcgaggtag ttccactgat
    22321 tgaagcgggc aacagcacgg ttgccgtcat tcttggcctg tgaaatataa ccacggacag
    22381 aagccgcatt cgggttggca agaatctggt tcaacaggct gttggaccac atcaggtaac
    22441 cagccatttc aatgcggtac atgttatcgc gatcgaactg accgaactca tcgctcagat
    22501 ccatatagtg catgatggtg ttgctttcat cgccagagtt cgcaaagtag aaatcatcag
    22561 acgcgccata atcaagaccc aattctgagt cgtaaccatc gtgcgggtga gacaaaccga
    22621 agtggtggcc gccttcatgg atggtggtga tggtaaaacc ataaccgcca gcgcgataat
    22681 catccgtatc aaacgcaaac acatagctcg gggtaccatc aatccagttg tcatcggcaa
    22741 agccaagcaa accatactgg ttacccagat tatcggcagt ggtgttgaac tcatggaaag
    22801 ccgcaacata gtcggcatcg gtgtattcgg ggatgtaggc gttgcggtta atatcaaaat
    22861 agcagaacaa ctgggcatac ggagtgcccc agtcattcca acaatcatcc gagccatcca
    22921 actcagccca aatgcggaag gacttttcgg cattggcatc aatcgggttg acatcggtca
    22981 ggttgacctg ccaattgtag taaggttgga actcagaaaa tctctgctga gccagaccag
    23041 ggttgatcca gtcagtaccc tgactggcgg gatcatcttc catcatattg atatgaacaa
    23101 tcttgctgcc gttaacttca ggggcgctcg caagtggatc atacaacggc gaggtggtga
    23161 agagcagatt gatccccaca aaacgggcga ccaaaccaag gtcggtgctt aaagcggaag
    23221 gatcacggta tccattggca gaatattccc aaatgggagg catacgataa tcttcaacgc
    23281 cgtttccatc caaatcaggg ttatcaacat catagttacc ggtccaggct tccggaccag
    23341 cggaaagatc atagaaccac agacggctgc tgcttccacc ccaagcaatg gtcttgcggg
    23401 aagagcggat ttcgccgaag ttgtagccag tatccggatc cgccacatcg gtcttggtat
    23461 acacatggaa tttgaaatcc gggcggccat accagttgat gaagacaatc gtgtacccct
    23521 tcttgttgac gttcaaattc tgattcaacc aactctcaac agaaggcgca tcagcataaa
    23581 ggacttcatc agccacatca aggacattat tttccatgtc attgtaggca ttctggtaaa
    23641 acgtggggcc cgccacagtg ctgccctggg tcaggaactt gaagaaacgg tcagtggttt
    23701 tttgatttac aaaagagacc gtgtaattaa aatcgaatct caacccaagg ttgcggcctt
    23761 caaggccata aaaggcagga tagcgcacaa tcggggtata cgaggcaggc agcagatctc
    23821 tgatcgcttt ctgattaatt gcgttcttgt catatcccaa aaacacgaca ttaatgggca
    23881 ctctctgtgt aaacatcgta accccaccgc caccaaaacc ctcctggggc gcaggagcgg
    23941 ccagcgctgt tgctggaatc agacccagca tcatcacaag aaccgcgata atagaaaaaa
    24001 gctttcgttt catactaatt ctcctttttg attcagaaat tgaaactcag atgctttcac
    24061 tagacaaact gtcagaattt acggttcgac ctcctctcca ccttttgaaa aaataattga
    24121 ctcaaaagaa cccataacat gcccgcctgt tttatgtctg aaatattctt tcgggtaatt
    24181 tatcattaca actcaactgc aattatcctc ccaaccgata aaatgtgcaa gtattttttg
    24241 agaaaatccc gctcttggcc gatttccaat ctgagtataa tgattggatg tcaaaacgca
    24301 ccatcatccc catttttctg ctcatttgca ttgccggagg gctgtattac tacttcattg
    24361 gcaggcgagg ctcagcccgc acagccgaag tctacaactg gctgagagat tccacatcaa
    24421 gacctgagtt gatgatgacc acagggacaa cctgtggcga cgccccattc attttcccca
    24481 ccaacggcct gatcggcttc atttgggacg attctttccg acccggtcac cgccacagcg
    24541 gaatagacat tttcgcaggg acggatgtcg gcatcacccc catcgtgacg gcataccccg
    24601 gttacctcac ccgcaaatca gactggaaat cgtcggtcat catccgcgtg cccgaagacc
    24661 cccttcaacc cgggcggcag atatgggtgt actatactca tatggcagac cccaacggaa
    24721 actcgtacat ttcacccgaa ttccccaccg gcaccaccga agtgttcgta gaggctggca
    24781 ccttccttgg ctatcaagga aactactccg gcgacccgaa caatcccgtc ggcgtgcatt
    24841 tacatgtttc ggtggtcaaa gatgacggtt tcggcaattt caccaacgaa cttgatatca
    24901 acaacaccta cgacccatcg ccctatttcg gcctacccct gaacgcaaac gaaaacacag
    24961 atacgatccc tgtatgcaat taattatgga ggctttatgg acttaaggaa caaagtcgtt
    25021 ctcatcaccg gagcagggaa gggagccggg gccgcgctgg cccgtgcttt tgcagaacaa
    25081 ggcgcgctgg tagcccttaa cgacatctcc cccatcaatg tagagcaggt tgcagctgag
    25141 atcacggcca aaggcggcaa agccaaagcc tatgttgaag atatcgccaa gaaggttggc
    25201 gtgcaatatg tgatcaaagg cgtggaagat gatttcggcg gcatggatat tcttgtcaac
    25261 catgcggtgg tgaaacccca taccaccctg ttggatgtgg atgaatggga ctggcaccgc
    25321 acactggacg taaacctcac gggcgcattt ctcaccattc aatcggcagg acgcatcatg
    25381 cgcgaaaaag gtcacggggc aattctgaat attgtggcag aggcaggcat aaggccagag
    25441 tcagaagcgg gggcatatcc tgccagcatg gcggggttgg ttgcgttatc gcatcaggca
    25501 gatcgtgaat taagtccgct agggattcgg gtttatgcgg ttgaaaattc agccgatgtg
    25561 gtgaagaatg tcatcaagat actggaggca caatgaaaga actgtacgag tatcgtgaaa
    25621 agatgatcgc ccgtctggcc caggctgcgc gggaattttg cgccgcatgt gaggccttcg
    25681 aaaatccgtt gacacaagtg gaaggcgagt ggaatttgca ccagatcgca tcacacgtcc
    25741 gcgatgtgga aaagttggta tatggcgatc gcatccgcaa gacgctggag atggaaaacc
    25801 ccgccttcca aagttttgat gccgattcgt ggatggaaac gaattacaac caaaatgaat
    25861 ctttgcaggc cattttgagt gaattctctt ccaatgtgga tgcgctatgc caaacactga
    25921 aaaacctgcc gcaatcagcc tggtcacgtg agagcagcca cgaaaccatg ggcggagaat
    25981 tggccttgca attgtgggtg gaacgcgggt tggcacacat tgaagagcat ttacaaacgc
    26041 taaaaaaggc ataaaatacg tgaaaatggc tcgttttctg catatttcga ggtaaaataa
    26101 cggcatgaaa aaacaaagaa tcattctcgt agatgatcat gaagtggtac gtctcggctt
    26161 gaaatcgctg ttggagcggc acccgcattt cgatgtggtc ggtgaggcag gctctgcccg
    26221 tgaagccctg gagcaagtgg aagccctcaa acccgatgta gtggtgatgg atatccgcct
    26281 gccgggcaca tcaggaattg atgcctgcga gcagatcgtc agccagtttc caaacacaaa
    26341 agttttaatg cttacatcgt atgcagaaga tgaaatgctt ttctctgcga tccgtgccgg
    26401 ggcttcgggc tatgtgctca agcagatcgg cagtgaagat cttatcaagg ccattgaatc
    26461 tgtcgggcgt ggcgaggccc tgcttgatcc cgctgtaacc caacgcgtct ttcaggaagt
    26521 gcgccgcgcc gtgaaagagg aagaggcttc agccttcgct catttgagtc agcaggaaaa
    26581 acatgtcctc ttgctggtat cggaaggcaa gaccaaccgc gagatcgcca agaacctgtt
    26641 cctcggcgaa ggcacggtac gcaattatgt ttcgagcatt ctctccaagc tcagcgtgaa
    26701 caatcgcgcc gaggctgcgg cctatgccgt gcagcacagt ctgagagaat acatcactct
    26761 gtaagaaccg cacgttcaac aacaaaggca caacacacgg agaatcaact cggtgcaggt
    26821 tgtgcctttt ttattttgcc ctcgtcctga gtgaaacgat cagcgttttt tgaaaaccac
    26881 ggtcagagga tgatacaacc cagccatgtt cccatttcta aaacttacag acggcaccat
    26941 caccctgcgc cctttcgaat ttggcgatga aaaggatctg cgcgacgctg ttcaggaatc
    27001 actgcccgag cttcagccgt ggatgtcatg ggccacagaa aaatacaacc ttgaaaccgc
    27061 gcgcaatttc atcgccatca cccgcggaca atggtcaagc ggcagtttgt atgcctttgc
    27121 catcgccgat gccgtcaccg atgaactggt cggcggatgc agtttgagtc acattcaccc
    27181 ggtctactat ttttgcaacc tcggctattg ggtacgcagt tcgcgccacg gcgagggcat
    27241 tgccggggcc gccgccaaac tggcggcaca tttcgcattc gaacgggtgg gcctgatccg
    27301 cactgagatc gtcattgccg ttggaaacga gcccagcaga cgggtggcgg aaaaaatcgg
    27361 cgcgcattac gaagggattt tactgaaccg aatggtggtg ggccaggcca ttcacgacgc
    27421 ccacatgtac tcgcttcttc cctccgactt tggactgaca gccaaacgat aagcagagtc
    27481 aacgcttcaa gagtgtttat acccatcacg gtcacaaatt gcgccttttc agaagcggga
    27541 aagcgcaatt tgtgaccgag ggcatatata atcaacccat gagcaaatca tcctcccctc
    27601 ttctctttct cggcaaagcg gatgaccccg attgcgcccg cgcactggaa ttttgccagt
    27661 cgcatttcga gcatgtaacc ttttgcctcg gcaaatgggg cgacccattg cccgaaacag
    27721 cccgcaactg ggaaggcgat atcatcatct cctacctttc acgctgggtg gtgcccgccg
    27781 atttgttaaa ccgcgctaaa atcgccgcca tcaactttca ccctgcctcg cccgaatacc
    27841 ccggcattgg ctgcaataac ttcgctctgt atgaaaacgc aaaagaatat ggcgtgacct
    27901 gtcatcacat ggcctccaaa gtggatacag gccgcatcgt cgccgtaaaa cgcttcccca
    27961 ttttccccga agatgacgtg gacgccctgc tcaaacgtac ttatgaaaat cagatcgccc
    28021 tgttcttcga gatcgcaaac ctgctcgccg aagggaaaga actccccacc tcggccgaaa
    28081 cctggacccg tccgcccttc tcgcgcaagc aattcaacga actcttcatc atcacccccg
    28141 atatgaacga agacgaagtg aatcgccgca tccgcgctat cagctacggc ccttggcagc
    28201 cgtacatgga aatcaatggg cacaggtttg agtacaaacc tgagaagtaa aggaaagaat
    28261 caaaaagagc gcggaaggca tccacgctct tttcattttc ttcgttctac cgattacttt
    28321 tccttctccc cgctcacctt catccatgcc ttcttcatat ttaacgcatc ctcttccatg
    28381 atggggcttt cacacaggat gc
//
LOCUS       DAOQJC010000048        27935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_741_length_27935_cov_9.715818, whole genome shotgun sequence.
ACCESSION   DAOQJC010000048 DAOQJC010000000
VERSION     DAOQJC010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 27935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27935
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_741_length_27935_cov_9.715818"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..1961)
                     /locus_tag="PLE39_09185"
     CDS             complement(189..1961)
                     /locus_tag="PLE39_09185"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNE68542.1"
                     /translation="MSDSIGQRLKKERENRFLTLERASAETRIRIVFLQALESDDYSV
                     MPSAAQGRGFLRNYAEYLGLNIDEMIAELQKKAAEAAQQQAVDGPLPQVNLSETELPP
                     LTDVKEEKPARPFLSWLGRRPKADSDTVSESTEPAPEETPAPVVEPVEPAAPLEIPVA
                     PVLETPPVEPVEKPKTRGRKKKKTPADEVEVEAVPVQPEPVQEEIAAVQADVTESQLD
                     ALIPSSEENQDEVKEEAKPSLLSRLLGIFPSRPAKSEPELRPVLETRPQQTVPLQPAD
                     VIFAEIGQQLRSRRELISLTYDEVERHTKLRAAFVKALEEGALDRLPSPVQTRGMLAN
                     YATFLDLDVDALLLRFADGLQARRHEKYAETPREKIQMEVVTSMPVLRSFIAGDLIFG
                     VLIISVLVALGIWGVGRVVTSQDEVVAEVTAPSIVQVLGGTPLPTSILNATATIEGTP
                     AETPTVDPLFTPEVPTPVSTANISVSIFAVERTFARVSVDGEVVFEGRFAPGETKLFE
                     AAEQISILTGNGAGLRVTYNGRDLGLLGNIGEVVSRVYLITGVAEPTATISPTPTVTV
                     PATITPVPSSTPSITPTVTPPDGG"
     gene            complement(2030..2575)
                     /gene="rplI"
                     /locus_tag="PLE39_09190"
     CDS             complement(2030..2575)
                     /gene="rplI"
                     /locus_tag="PLE39_09190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNE68543.1"
                     /translation="MKVMLVKDVYKLGRAGDIKKVADGYGRNFLIPQGFAVLATEGAL
                     KQVQKIKAQAEIRRTAQNEELKGLADQIKAVTLTFAAKAGDTGKLYGSITTQDIATAL
                     TEKVRFEVKRQQVDIQPIRNLGEYNAHVRLTMDLVPEVKIIVHRDGESAEPAAEEEKA
                     DKKKKAKEETAAEEAPAATAE"
     gene            2700..3077
                     /locus_tag="PLE39_09195"
     CDS             2700..3077
                     /locus_tag="PLE39_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68544.1"
                     /translation="MPNLPISLRRAATFFGIFVLILVVIEFNTRLEELNRLNDQRDQV
                     RAIATQAMQTQVALNTQVAYAESTAAVDEWARTQGHYVQEGDQPVIPIGQPGSEPVIV
                     STPIPTPTPMQNWEVWWSLFFSE"
     gene            3086..5638
                     /locus_tag="PLE39_09200"
     CDS             3086..5638
                     /locus_tag="PLE39_09200"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNE68545.1"
                     /translation="MLIDLGLFSFYLGVLVLLTLGIAGGAFLNWERPGARTFGFLMLS
                     MMIWAAFYLCEIIAPSLPLKILCRKIMYLGMGISPPLWLGFALRYTGLAPWWSKRGRV
                     FLMTLPGLASFFLGLTNEIHGLIWKSLEIPPGHEIGPLHVIFGTGFWFFTVIAYFFIG
                     MGFLVYIYAFIRSPKTFRIQTGIMLLGALITLIVNLTFVAEPSNNHIDPTPLSFALSA
                     PLLAFGFFRFGLYNLFPIAAPVIMENLHDAIIVTDSQNRITNLNPAAKEWMNAKGQIV
                     GMNIFEAIPNAEMFMEKWDITNEPIKFRLNRSDGVRTWYEASVIPLHKNNAELLGRVL
                     VVHDITQEQELLESEFRRSAHLGLLEEVGRQIASSSDEMEILQHSIHAVVDRFGYGEA
                     AISVLTPDNMMEIRVISGTHDFGYDPGFRQEFGKGIIGHTAKTRKTYVAKNVAEDPYY
                     FSNDEHHGSAICVPILIENKLYGALYVETIESDAVTSEDITTLETLVNQISASLQRVA
                     LYSKAQEHLRVMSAVQAVSRVVISSLDLETIFETVVRELKNSFGYSHVSIYLLKDEYL
                     YLGAQVGYPLEMIIHKIHISQGVSGRTIKTKAVQFIPDTSREPDFLRADQTVRSEICV
                     PLLKENVVLGTLNVEGDANDILTREDVELLTTLSSPIALAVDNARLHAQVKSMAMTDA
                     VSGLSNRHALEEYLTAEVERSTRLGHPLSLIIFDIDSFKEYNDKWGHPAGDTRLRAIA
                     DLIRGILRKYDVAARYGGDEFAIILPNTDEHGALEFARRLQNTARAKAAEIHEERKVI
                     PGYTLSIGVATFPKDGNSLAGILLAADHAELTAKRLGKNQIFVASNLYKNDE"
     gene            5631..6917
                     /gene="lysA"
                     /locus_tag="PLE39_09205"
     CDS             5631..6917
                     /gene="lysA"
                     /locus_tag="PLE39_09205"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNE68546.1"
                     /translation="MNRIDLFPVSAKIEQDRLTIAGHDLELLAKEYGTPLYLYDQKTM
                     DDAALEYKNALASHYPGPGSVTYAGKAYLCTAIAQWAHGHELYVDCTGEVEIAFAQAG
                     GVPREHILVHGVNKSQADLKSALQHAGTIVVDNLTELKNIAAVSTVASNLWFRLLPGL
                     AVETHHAHTQTGQHGSKFGMTRTEIMEAADICRQHHLPINGIHFHQGSNFRDARPLKE
                     AIGIGLDIAKEIGFPDVWHFSPGGGWGVAYHEDELPQPDTNEYVRVIAENVLAGCQSR
                     GLTLPHLHLEPGRSLVARAGVSVYRAGAIKKRDEKTWVLVDGGMTDNPRHAMYGARYS
                     CLPVSGVGAERSGRISIAGPFCESGDVVIEDLPMRDIHEGDLIAIPASGAYQLSMSSN
                     YNGSRRPAVLWLEKGNARLIQRRETIQDLLARDVMI"
     gene            complement(6914..11761)
                     /locus_tag="PLE39_09210"
     CDS             complement(6914..11761)
                     /locus_tag="PLE39_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE68547.1"
                     /translation="MKSLFRPIFFFDPSISRSLQEWRELVLAALLRIVLLIWILSLIA
                     GVNNVFKSYILEVDKTSNPLLQAGIVLSVYLLATFTLVLITFGKNLPYNLRAWGLLFA
                     VYIVGAIGLVFSSLSGDGRVILFAFIILAAVFFDLRYSVPAFAIGLATMVIVGYLQVN
                     GILVIPPERQLISTDSSSWISGVVVFLTLNIAVLISITNLLRVLGTNLEKSQELLQRE
                     QRLSQILRTVTGINQLIVREQDPHQLLSEACILLVSGQGYSYAWAGLLESDQMTLKLA
                     AQAGDKIDPALFNVRIDELNERGLACAANAIKSRSFFRVDPIKGEDDPCKGCPRRIDN
                     PERVGVALPLLREDRVLGVLVVDHAVPEAVFEDEEIHLLQDLANDLSYALEKIEVNDR
                     LQVYARHQGILNAITQVALETPSLEAMLQKLIENLEGMFGAEGCYFSLWNEETQQVEK
                     FISSDIYKNILGTRPNLNPGEMTFTQSILENRQILVVDDIMNSPFISPRVASLFPVFS
                     AVGLPLIANEKKLGALIFGFRESAPPLSGDDIALAEQTARQIALAMLKVRLDEDTRAK
                     AAELERLYAAAQDMSSSLLDAPELLEKLAHHMTEALSVTSGNIMDINLVTATMQVVGE
                     YWSAEASPREVHSDLGRVFQNEGYGSIIKSMLAGEVLIMHSDDEEMTPTEREQFSEYG
                     ICSMMFVPILAHGQLFGNIEIWESRRRREFSQADIRLAKAMAGHAASIIENAKLVNAV
                     RVSETRYRNLVEQASDGIFLVNPEKMILDANPSGAAMLGYSIEELLAMSVDDIVIAGH
                     SNLLIDELRMGRSMIVEQYVRCKDGSTLPVEISARMLPNGYFQGIVRDISERKQAEKA
                     LAEREAYFRALIENSAEGVAILDEEGVVRYIAPSDEKLTGYSANNVQGTSGFQYIHPQ
                     DLPGVMKIFKEGVATPGAVRTTEYRLRRMDGEWRYFEITGHNMLDDPHIHGVVVNYRD
                     VTERKLAEQALQESRSRLEAIISSALNGIITIDESQHIILFNPSAERIFGYATSEVIG
                     QPLSKLIPDRYHASHGVHVKDYAKSGISARQKGLLDSLFGKRANGEEFPMEAFISNSQ
                     VAGERFFTVIFQDITERRLAEDALRESERKFRALAGNIPSTVYQCKNDERYTVIYLND
                     SVEMLTGYPKEDFLEGGLSFVDLYHPEDAKQIPIPSFVGEDTSSSDLSFHISYRIRHR
                     SGDWRWVDEWGVGVLDDRNAVQYLEGVMIDVTDRKNAEEELRRHAMELETLSVASSAL
                     RTAQNVSEMVPILARHALQAVGGTFASIFLLDSETQDFVSHGWYSVDPGVNFTLADES
                     EVRHKPGTGITGRVALTGEIYITEDIQKDSVITILDGERERLMTVHGGISLPLRTQEK
                     IIGVMHVWSSEPRIFNETDIRLLTALAENTSNAIHRAALFEQTLNHASELATAYDNTL
                     AGWARALELRDELTEGHTRRVTELTIKLAQALNIPEAEIVHIRRGALLHDIGKMGIPD
                     SILHKPGAFTAQERKIMQQHTQYAYDMLAAIPFLQAALDIPYCHHEHWDGAGYPRGLK
                     GTEIPLAARIFSIVDVWDALTSDRPYREAWSKEKTRQYILERSGKQFDPQIVEAFFAF
                     VAQSD"
     gene            11998..12597
                     /locus_tag="PLE39_09215"
     CDS             11998..12597
                     /locus_tag="PLE39_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68548.1"
                     /translation="MKDIVFLLLTGLLAAYLCWYFYQRYQLHKALHNLYYLMGFAVLL
                     VSGLLLIVFGLGILSSPYVLTVASLIPLGVSMGLAEEYFPAWKKYFKWFAALGFLAIA
                     VTSIGGMDLLKKIAVPLFHGVAGLVIFIGPFYAKGAPKGFFWVGIGGLLIGLGGIALA
                     FITLGKQLLFFSPEFVTLILTPLLFLMTGAYVLGFAKRA"
     assembly_gap    12600..12699
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12700..14136
                     /locus_tag="PLE39_09220"
     CDS             <12700..14136
                     /locus_tag="PLE39_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68549.1"
                     /translation="STKGLILVAIAMVGIVTAVWGMLSGPMAEMGVREIVIKTLGMKI
                     VQAEREGRIIILYHSIAMAIIAIETYMVTGLIKMKPFFKTAINATITVGYILTMVFGM
                     GFAYFGRNWAFHGLYITGLSLIFFAGLMLIVALWPWNPEYHVTDKSYAHTKSGLDLER
                     TAFFATAVTTVISSLFGAVPGSYYGHGFETFLAENIIRLPEKTVMEYSVIGHLHIMLA
                     LIGIMITLIIGRWLNFKGAWHKAAMPLMILGTIVLNLGVWGVVTPLEPVAHMVIYVGA
                     TPSMLAALFLLIWSWAKLIKDGTAHLTKPTFGQKVGALLRDPLKFGPTWQMLFMNFTT
                     SGLGIFMAIKLDEIFRVWPAREERIELTGHWHVLSAIIATIILLYYGDMNGFKGRLRQ
                     WYGWGIIILSDIAFGAVTLFEMKRLFVTEAAQQPLVNGLMYAIDFGLGMLLVILAIIM
                     VHRLIDLFKNKGRWIEELDHELAQEVDK"
     gene            14133..15029
                     /locus_tag="PLE39_09225"
     CDS             14133..15029
                     /locus_tag="PLE39_09225"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE68550.1"
                     /translation="MKRFTLTLCLCAALLASCAPVDLNAPIPTFETGIDPNTWAQIPA
                     GEFHHGQFDETASTEAYEIMVTDVTAAQYADFLNAALADGSIKMDGGKIVGFYPGDTF
                     HGVKHEEKIEAGDWLFIPLDDPSQRIQFDGTTFTVQSGYEKHPMTSVTWFGAWGYCQY
                     FGTRLPTELEWEKAARGTDERPFPWGDEIQRGHANFYSSRDPFEDMASFGSRTSPVGF
                     YNGQVYDGFQTIDSASPYGLYDMAGNVWQWTGDVYEGMHYRFMRGGSKDTYDMDLRIW
                     VRNNATPTYFSPGVGFRCARDE"
     gene            15130..15999
                     /gene="cyoE"
                     /locus_tag="PLE39_09230"
     CDS             15130..15999
                     /gene="cyoE"
                     /locus_tag="PLE39_09230"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNE68551.1"
                     /translation="MQAQLSFSTIRSKLGLYIPLIKSLQTSLLLLTGVAGYLSAHTTV
                     NWLHFWQMLPSLFLAISGSTILNMWWDQDIDAKMKRTHKRPTSAGQVTREEVFNLGMA
                     VSVIGIGWAVMVNWLYGLVVFAGLFFDVVVYSMWLKRRTCWSIVWGGISGAMPILAGR
                     VLAVGQIDVIGILLACAVLFWIPTHTLTFSLKFFDDYQSAGVPTFPSTYGEAVTRFAI
                     AFSSVVAALTMGWASVWIGVTAGVLRLIIVLSAGLFFLAITTVFRPSDRVNFSLFKYA
                     SMYMLFAMLLIIP"
     gene            16032..16637
                     /locus_tag="PLE39_09235"
     CDS             16032..16637
                     /locus_tag="PLE39_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68552.1"
                     /translation="MKMSSLDRTLLLFTGFLAAYQIAIGIDQMDSVSITAYTIAFGVI
                     LVAGLLLIILGFEVLDAPIVVIISTIIPLALSLGLIWQHLASLRTVYLIFTIISFLAV
                     VLTRSIAMQNKLPVIVIAITHGIAGLTIFLLPIILAAQGQVKPLFSLVGIGGALIGIG
                     GLLLSFLKTGKPILSRDTIMRLFPMLLLLTTALFVAGFKLG"
     gene            16695..17372
                     /locus_tag="PLE39_09240"
     CDS             16695..17372
                     /locus_tag="PLE39_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68553.1"
                     /translation="MSANNFFAKSLRFIGIVLMALTGGFTFLGGAGTFCAAVFPQKYE
                     SMAGLIPYQWLYILFMLGTIAIGVWGIWAAIKLIKGTVDAYRMSLYALITGVLVGGFH
                     IYMSRMLRGKSMPVDAVVYTTLLTLVIFLLFKIPMIWQGVDFSKAKASDNKKAGGAAA
                     FVVGIFTLTIQYTMGPTHTWDGINYADAFNTAMTVIGIGLLVLGTGIFVSLGNVGKTA
                     LRLQVQQ"
     gene            complement(17448..17927)
                     /locus_tag="PLE39_09245"
     CDS             complement(17448..17927)
                     /locus_tag="PLE39_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68554.1"
                     /translation="MDILQLIDRLEELFNAAKAVPFTHNVIVDEDRMLELIDQMRIAI
                     PEEVKKAQQIMAQRDRVMAQAQEEANRTLQLARDKADQLTQKDMVVQEAQRRADQIIN
                     QARGEAEATRADADNYVVDTLMQLQDQIAKMSSQVSNGIRMVQEDQMRKSVSSEKVE"
     gene            complement(17990..18505)
                     /gene="coaD"
                     /locus_tag="PLE39_09250"
     CDS             complement(17990..18505)
                     /gene="coaD"
                     /locus_tag="PLE39_09250"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNE68555.1"
                     /translation="MVRALFPGTFDPIHYGHMDIASRASKLFDEVVMAVYDKPLKSLM
                     FSPEERISLVTEAFKDNPKIKVTGYSGLTIDFARKIQAQVIVRGLRVFSDFEFEFRMA
                     LANQRLAKDIETVALITAEQHTFLSSSTVREIAMLDGDVSSMVPAHVKSALHVKVINM
                     GEQSPPNALRD"
     gene            complement(18595..21162)
                     /gene="recG"
                     /locus_tag="PLE39_09255"
     CDS             complement(18595..21162)
                     /gene="recG"
                     /locus_tag="PLE39_09255"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNE68556.1"
                     /translation="MQPSLEKLRKFFRLEHENKYENTAIIGGLAKMLDYWEGEARADG
                     VAEEVIQAVAARLRSYEKLSPDGRAESLKGLWKRIGETYPEAGQKPKAPPQPSKANQP
                     PRPPRPEPTDPAQLEAPTPPVPPESKPQPRPQAQEQRKPRPTPPPQQQRSRFESAPGA
                     KTSQTPAALNADITVLQGVGPKHAESLKSIGIFTLGDMLYYFPRRYEDYSQLKPIKSL
                     FYGEAVTVLGTVQSVHTRPIRGGKSSIVEVIINDGTGGLRLSFFNQPWLINRMKEGEA
                     ISVSGKVDQYLGRLIMNSPDWEPIEVENLHTARIVPIYALTEKVTQKWLRKLMNEVIP
                     FWAPAVVDILPEKIKLSARLVSLSEALMQVHFPVSQSRLKAARERLGFDEIFFLQMGV
                     LRQKRDWKDAEARRFSVSTEWMDSRLSGLPFTLTSAQQKSLEDIRADLNSGRPMNRLV
                     QGDVGSGKTVVAALGSSVVVSEGAQAAIMAPTSILAEQHFRNFSNLLAGPQGFLQQSE
                     IRLLVGNTTESEKESIRQGLADGSVKIVIGTHAVIEPDILFKDLQFVVIDEQHRFGVE
                     QRKELRSKGTNPHLLVMTATPIPRSLALTVFGDLDLSVIDEMPVGRQPIDTYVLRPQE
                     RERAFTMIRGQIRSGRQAFIVYPLIDESEKIEARAAVDDFDVLSKEVFPDLKLGLLHG
                     RMRPSEKDEVMLKFRDREYDILVSTTVVEVGVDVPNSTVMLIEGADRFGLSQLHQLRG
                     RVGRGAAQSYCLLIPTHEDATENERLQAMAKTNNGFELADLDLKTRGPGEFLGTRQAG
                     FGTQLKMASITDVSLIEKARTQAQALFERDPYLQNPEHALLAEAFERFWGTGKGDVS"
     gene            complement(21188..22024)
                     /locus_tag="PLE39_09260"
     CDS             complement(21188..22024)
                     /locus_tag="PLE39_09260"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNE68557.1"
                     /translation="MKIGIVTDSTSDLPAHLIEQHEIHVIPSILVLEGKEYMDGVNIT
                     REEFYTRLPALRTPPTTAAPSIGDFTAPYEALLSKGCDHIISIHPAVKLTTTINVARK
                     AAEEFPGKVTCVDSGSLSLGIGFQILAAAEAAEFGLQSALDAITSTQKRLHVFAALDT
                     MEYLKRSGRVPGAVAALGGLLSIKPMIELKDGEVKPVGAVRITRQADERILNLLLERG
                     ELERLAILHTNAEPRAKNLLDDLMNQARKSVPREILFVNVTAVIGTHLGPNGIGFAAV
                     RK"
     gene            22115..22444
                     /locus_tag="PLE39_09265"
     CDS             22115..22444
                     /locus_tag="PLE39_09265"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE68558.1"
                     /translation="MANNESLENVTLELRRGVIVLAVLSQLSSEQYGYSLLKLLSDQG
                     LEVDQGTLYPLLRRLETQGLLESVWKLEEARPRRYYIISAEGKKILPKLKKEWAGIVA
                     VMAKVLA"
     gene            22454..23473
                     /locus_tag="PLE39_09270"
     CDS             22454..23473
                     /locus_tag="PLE39_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68559.1"
                     /translation="MNLIDRYVTEVGKNLPILKGREDIEKELKSTLEDMLEDRVEQTG
                     QPRSEAMEIELLKEYGSPQKVAATYNPQPYLIGPRLFPFFLMVLKIVIPILVTVLLVL
                     TGIRAATDTPFMGAGFMNIVSKGLGGIVSAAITAFGNIVFIFAILERVLPDKEIGDFS
                     NEKDWDPATLAKEPDPDTAKRSDLIAEIVFTFIGLAILNLFPDILGMSYFAEDKMVFI
                     PMFSAEFFKFLPWINAVFLAEIVLDIYLLRSAVWTSITRIAKIIIEVASIAVAVAILR
                     TPNIFGFTAESFQSVPNSSINADLLLNIFTLSFSIAIIIAIIVAGVDMAKAAFGLLRM
                     NYKRK"
     gene            complement(23575..25242)
                     /locus_tag="PLE39_09275"
     CDS             complement(23575..25242)
                     /locus_tag="PLE39_09275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HNE68560.1"
                     /translation="MGVDSARVEKFRRMPIDGQSLKRLIDAGLTWLRTNKETVNALNV
                     FPVPDGDTGTNMVLTLQAAWNEVKDSGARNLGEMAAMVSKGALMGARGNSGVITSQIL
                     RGFSRGVHEKATLDVPTLVKAFGEARDTAYKGVVRPVEGTILTVIKEVANATEAALGS
                     AAERDAIEILEVAVKAADEAVKKTPEMLPVLKQAGVVDSGGKGLFFILEGMLRHVYGE
                     SLETPTMSVKPMSAMNLQGALEEVEEGQDYEVVVDFVPTGELDLGAFYGRLEEMGTSI
                     QVGEGEGMYRMHIHVPTEKRYEPIDYIMGIGTVTKVAIENLLAQMDDIQKSKQIQFAT
                     VEPGHIAVVVVSPGAGISRIFASLGVAAVVAGGQSMNPSTQDILSSFENLPTDKIIIL
                     PNNKNIILAANQAKEVTVKQVAVVPTRTVPQGLAAMLSLIPDGELDSVADKMTKALGS
                     VKTGEVTIATRTVEIDGVSVKDGQVIALLDGKLVVSAESVEQGVMELLEKADADQHEL
                     VSLYYGEGMTHAEANRIADLIVTKYTALEVEVQEGGQPHYQFIISIE"
     gene            complement(25258..25614)
                     /locus_tag="PLE39_09280"
     CDS             complement(25258..25614)
                     /locus_tag="PLE39_09280"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNE68561.1"
                     /translation="MGDETTTLGGIHISPNAVATIAYYATLQSYGVVGLAPKNLADGI
                     VSTITREPSRGITVRYKGEDIDIDINVVVEYGTRINSVAVSVANTVRFHVEKALGLRV
                     NSVNVHVAGLRISDTD"
     gene            25867..26052
                     /gene="rpmB"
                     /locus_tag="PLE39_09285"
     CDS             25867..26052
                     /gene="rpmB"
                     /locus_tag="PLE39_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNE68562.1"
                     /translation="MAKCSTCGKATTFGHNRSFSLRATNRTFKPNLQKVSVMENGRLV
                     KKTLCAKCIKTLGKSAA"
     gene            complement(26113..26772)
                     /gene="rpe"
                     /locus_tag="PLE39_09290"
     CDS             complement(26113..26772)
                     /gene="rpe"
                     /locus_tag="PLE39_09290"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNE68563.1"
                     /translation="MNKFLLAPSIIAADFTRIAEEIAACESAGADWLHVDVMDGHFVP
                     TITIGPLFTEACKRVTKLPLDVHLMISEPEKYLEAFAKAGANNITVHVETCPDLVGTV
                     KKIKSLGCKAGITLNPATPASALDSALPLVDLVLVMSVVPGYSGQAFMPEMVGKVEEI
                     RARLDALHSTAHLEVDGGMSAKTLPLMYKAGADMFVAGSAAFGHPQGVGQGIKILREC
                     V"
     gene            complement(26814..>27935)
                     /locus_tag="PLE39_09295"
     CDS             complement(26814..>27935)
                     /locus_tag="PLE39_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE68564.1"
                     /translation="MTDETLLNDRYQLLERLGTGGMADVFRARDLMLDRYVAIKVLRK
                     DYSTNEEFQKHFRMEARSAANLSHPNIVTVHDFGFADDLLYIVMEYIPGKDLKQLIRE
                     RGRFSVETGIPLIIQACAGLGYAHRAGLVHCDVKPHNMLVSEDGRLKVTDFGIARALA
                     TMTPGERTDIVWGSPLYFAPEQAQGEAPSPASDVYSIGVVMYELLCGTPPFTASTSDE
                     LARLHISARPIPIREYIPDIPTALEEIIMKVLSKEPAARYRTADQLGRVLLKFGTQPD
                     APAPAPTPVPPPATVISVSPEISDKLPTKETVSTSRQTTAPRQYTRPEPAPQESPEPV
                     SAFEEIDWVFWGLALFAVLAVGGLIPFWMMVYFAYNPPI"
BASE COUNT         6559 a         7211 c         7149 g         6916 t
ORIGIN      
        1 ggctcatcca ttgcgcgata tccatcacgg atcaataact gtgccattga atcagaatca
       61 actgtatttt ttgcacaacc caacgaaacc aagtgaaatg tattttttga catgtgtttt
      121 cctgactatg acttgagtaa cgaataaaaa aaacgaaacc tattcctggc gaaaatcaaa
      181 tatgttaatt acccgccatc gggaggtgta acggtcgggg taatggatgg cgtggacgat
      241 ggcacaggtg tgatcgttgc aggcacagtg accgtgggcg tcggcgaaat cgtggcggtg
      301 ggttctgcca cacccgtgat caaatacaca cggctgacca cctcgccaat atttccaagc
      361 aaaccaagat cacgcccatt ataggtcaca cgcagcccgg cgccattgcc ggtcaaaatc
      421 gaaatttgct cggcggcttc aaataatttt gtttcacccg gggcaaagcg gccttcaaag
      481 accacctccc catccacaga aacgcgcgca aacgtgcgct ccacagcaaa aatgctcacc
      541 gagatatttg cggtcgaaac cggggttggc acctcgggcg taaacaacgg atccactgtg
      601 ggggtttcgg cgggtgtccc ttcaatggtg gctgtggcat tcaagatgga agtgggcaac
      661 ggagtgccgc ccaacacctg cacgatcgaa ggcgcagtga cctcggcaac aacttcatcc
      721 tgcgaagtga ccacgcgccc cacaccccaa atacccaaag ccaccagcac tgaaataatc
      781 aaaacaccga agatcagatc accagcgatg aaacttcgca aaacaggcat cgaagtcacc
      841 acttccattt ggattttttc acgcggtgtt tcagcgtatt tctcatggcg gcgcgcctgc
      901 aacccatctg caaaacgcag aagaagagca tccacatcaa ggtcaaggaa ggtggcgtaa
      961 ttagccagca tgccgcgcgt ttgcacgggt gaaggcagtc gatccaacgc gccttcttcc
     1021 agcgctttca caaaagcggc gcgcaatttt gtatggcgtt ccacctcatc gtaagtcagg
     1081 ctgatcagct cacggcgcga acgtaattgc tgaccgatct cagcgaagat aacgtcggca
     1141 ggctgcaacg gaaccgtttg ttgtggtctg gtttcaagaa caggacgaag ttcaggctcc
     1201 gattttgcag gacgggatgg gaagattccc agcaatctgg aaagcagact cggcttcgcc
     1261 tcttccttca cctcatcttg attctcttcg ctggagggaa tcagcgcatc gagttgactc
     1321 tccgtcacat ccgcctgaac agcagcgatt tcctcctgaa cgggttcagg ttgaacaggc
     1381 actgcctcca cttccacttc atcggctggc gtttttttct ttttacggcc gcgtgtcttg
     1441 ggtttttcta cgggctcaac tggtggagtt tccaaaacag gtgcaacagg gatttcaagc
     1501 ggggcagcag gttcaacagg ttccaccact ggagcaggcg tttcttctgg tgcgggttcg
     1561 gtggattcgc tcacagtgtc agagtccgct tttggacggc gacccagcca ggacaagaaa
     1621 ggacgcgcgg gtttttcctc tttcacatcg gtaagcggag ggagttccgt ttcagaaaga
     1681 ttgacctgcg gcagaggacc atccactgcc tgttgttgtg cagcctcagc ggctttcttt
     1741 tgaagctcgg cgatcatctc gtcaatgttc aagccgaggt attccgcata gttgcgcaga
     1801 aaaccgcgcc cctgtgcggc ggaaggcatt actgaatagt catccgattc gagggcctgc
     1861 aaaaacacaa tgcgaatacg agtttccgca gaggctcttt cgagcgtgag gaaacggttt
     1921 tcgcgttctt ttttcaaccg ctgaccaatc gaatcagaca tgtctttatt ttactgtgct
     1981 tcagggaatt ataaatatca ccgacttgcg ccggtgatat ttgggtcttt tattcggctg
     2041 tagcggctgg tgcttcttca gcagctgttt cttccttggc ctttttcttc ttgtcggcct
     2101 tttcttcttc ggcggcaggt tccgcagatt cgccgtcgcg gtggacgatg atcttgactt
     2161 caggaaccag gtccatggtc aggcggacgt gtgcgttgta ttcgccaagg ttgcggatgg
     2221 gctgaatatc cacctgttgg cgcttcacct caaagcggac cttctcggtg agggcagtgg
     2281 cgatatcctg tgtggtgatg gagccgtaca atttgccggt gtcgccagct ttggcggcaa
     2341 aggtgagggt gacggctttg atctggtcag cgaggccctt gagttcttcg ttttgagcgg
     2401 tgcggcgaat ctcggcctga gccttgatct tttgaacctg cttcagtgcg ccttcggttg
     2461 ccaatacggc gaaaccctgc gggatgagga agttgcggcc ataaccatca gccaccttct
     2521 tgatatcccc ggcgcgaccc aacttataaa catctttaac aagcattact ttcatttcgt
     2581 tcaagccctt tctgggagtg agatatgcag agcgaagggt actacgttct gcgggggcag
     2641 attgtaccat aaagggggct tttcttgcca agccgggcaa atccgattaa gatacaggca
     2701 tgcccaacct tcccatcagc cttcggcgtg ccgccacttt ttttggcatt tttgtgttga
     2761 ttttggtggt gatcgaattc aatacacgcc tcgaagaact aaaccgccta aacgaccaac
     2821 gcgatcaggt gcgtgccatc gccacacagg ccatgcaaac gcaggtcgca ctgaatacgc
     2881 aggtggccta tgcagagtca actgcagctg tggatgaatg ggctcgtaca caagggcatt
     2941 acgttcagga gggcgatcag cctgtcatac caattggtca accgggcagc gaacctgtga
     3001 tcgtctccac tccgatcccc acgcccaccc caatgcaaaa ttgggaggtg tggtggagct
     3061 tgttctttag cgaataaacc tcttaatgct gatcgacctg ggactgttct cattttatct
     3121 tggggttctg gtactcctaa ccttgggtat tgcggggggc gcattcctga attgggagag
     3181 acccggtgcc agaacttttg gattcttaat gctgtccatg atgatctggg ctgcattcta
     3241 tctttgtgaa atcatagctc cttctctccc cttaaagatc ctatgtagaa aaatcatgta
     3301 tctcggtatg ggtatctcac cgccattgtg gttgggattt gccctgcgat acacggggct
     3361 tgcaccgtgg tggtcaaagc gcggacgggt attcttaatg acacttccgg gattggcttc
     3421 cttttttcta ggattgacca atgaaattca tggtctgatc tggaagtcgc tcgaaatccc
     3481 ccccgggcat gagatcggtc ccctgcatgt catatttggt acagggttct ggttcttcac
     3541 ggtcattgca tattttttca ttggcatggg gtttcttgtt tacatttatg cttttatccg
     3601 ttcgccaaaa acctttcgca tacaaacggg aatcatgtta ttgggggcac ttattacgtt
     3661 gatcgtcaat ctaaccttcg tcgctgaacc ctccaacaac cacatagacc ccactcccct
     3721 gtcttttgct ctatcggctc cattattggc cttcggattt ttccgctttg ggctatacaa
     3781 cctgtttcca atcgctgcgc cggtaatcat ggaaaacctg cacgatgcga tcatcgtgac
     3841 cgattcacaa aaccgcatca ccaacctgaa cccagccgca aaagagtgga tgaatgccaa
     3901 gggccagatt gttggcatga acatcttcga agccattccc aatgcggaaa tgttcatgga
     3961 aaaatgggat atcacgaatg aacccatcaa attcaggctg aaccgctcag atggtgtgcg
     4021 gacctggtat gaagcctcgg tcatccccct gcacaagaac aatgctgaat tattgggacg
     4081 tgtgctggtg gtacacgata ttacacagga acaggaactg ctggaatcgg aatttcgccg
     4141 ctcggcccat cttggcctgc ttgaagaagt tggcaggcag atcgcaagca gttccgatga
     4201 aatggaaatt ctccaacact ccatccacgc ggttgtagat cgctttgggt atggcgaagc
     4261 cgccatatcg gtcttgacgc ccgataacat gatggagatc agggtcattt ccggcacaca
     4321 tgatttcggc tatgaccccg gtttcaggca ggaatttggc aaaggaatca tcggtcacac
     4381 tgccaaaacc cgcaagacat atgttgcgaa gaatgtcgct gaagatccct attatttctc
     4441 gaacgatgaa catcacggct ctgccatatg tgtgccgatc ttgatcgaaa acaaattata
     4501 tggagctttg tatgtagaga ccatcgagtc tgatgcggtc acttctgaag atataaccac
     4561 actggaaact ctggtaaatc aaatctccgc ttcattgcaa cgggtggcgc tttattcaaa
     4621 ggcacaggaa cacctgcggg ttatgtctgc ggttcaggct gtctcgcgtg ttgttatcag
     4681 ttcgctcgac ctggagacca tctttgaaac cgttgttcgt gaattaaaaa attcctttgg
     4741 ttacagtcat gtcagcattt atttgttgaa agatgaatat ctttacctcg gcgcgcaggt
     4801 cggttatcca ttggagatga tcattcataa gatccacatc agtcaggggg tcagcggacg
     4861 taccatcaaa accaaggccg ttcaattcat cccagataca tcacgggaac ccgatttcct
     4921 ccgtgcagat caaacggtca ggagtgaaat ctgtgtgcca ttattaaagg aaaatgttgt
     4981 ccttggtacg ctcaatgtgg aaggcgatgc aaacgacatc ctcacacggg aggatgttga
     5041 attactaacc accctctcaa gccccatcgc cctggcagtg gacaatgccc gcctgcatgc
     5101 acaagtcaaa agcatggcaa tgaccgacgc cgtcagcggc ctctccaacc gccacgcatt
     5161 ggaagagtat ctcaccgccg aagtagagcg ttccacgagg cttgggcatc cgctttcgct
     5221 gatcatcttt gatatcgact ctttcaagga atacaatgac aaatgggggc atcccgccgg
     5281 ggatacccgt ctgcgtgcca ttgcagacct gattcgcggt atcttgcgaa agtatgatgt
     5341 cgctgcacgc tatggcggcg acgaattcgc catcatcctg cctaacaccg atgaacacgg
     5401 cgcactcgaa tttgcacgcc gcttgcaaaa tacagcccgt gccaaggcag ctgagatcca
     5461 tgaagaacgc aaggtgatac ctggttacac tttaagcatc ggcgttgcca cctttcctaa
     5521 agatggaaac agcctggctg gcatcctcct cgctgcagac catgccgaac tcacggcaaa
     5581 acgtctcggc aaaaatcaaa tcttcgttgc gagcaacctc tataaaaatg atgaatagaa
     5641 ttgacctttt ccccgtttca gcaaaaatcg agcaagaccg tcttaccatc gccgggcacg
     5701 accttgaact attggcgaaa gaatatggca cccccctgta tctgtacgac caaaaaacaa
     5761 tggatgatgc cgcccttgaa tacaagaacg cgctcgcttc gcactacccc gggcctggtt
     5821 ctgttactta tgctggcaaa gcctaccttt gtacagccat tgctcaatgg gcacatggac
     5881 atgagttgta cgtggattgc accggtgagg tggagatcgc ctttgcccag gcaggcggcg
     5941 taccgcgtga gcacatcctt gtacatggtg tgaacaaatc acaggccgac ctaaaatcag
     6001 cgcttcaaca tgcaggtacg atcgttgtag acaacctgac cgaactaaaa aatattgcgg
     6061 ctgtttccac tgttgcatca aacctttggt ttcgccttct gcccggcctt gcagttgaaa
     6121 cccaccatgc ccatacccag accggtcaac atggcagcaa attcgggatg acccgcacag
     6181 agataatgga agccgcagat atatgcaggc aacaccacct gcccattaat ggaatccact
     6241 tccatcaggg gtcgaatttt cgagatgccc gtcctttgaa agaagcgatc ggcattggtc
     6301 ttgatattgc aaaagaaatc ggatttcctg atgtttggca cttcagtccg ggcggcggtt
     6361 ggggggtggc ttatcatgaa gatgaacttc cccagcctga tacaaatgag tatgtgcgtg
     6421 tgatcgcaga gaatgtcctt gcgggctgtc agagccgcgg attgaccctc cctcatttac
     6481 atctggaacc ggggcgcagt ttggttgcac gggcgggtgt gtctgtgtac cgcgcaggtg
     6541 ccattaaaaa gcgcgatgaa aaaacatggg ttctggttga tggcggcatg acggataatc
     6601 cgcgccatgc gatgtacggg gcgagatatt cctgtttacc ggtatcaggc gtcggcgcag
     6661 agcgaagcgg acggatctcc atcgcagggc cattttgtga atcgggggat gtggtgattg
     6721 aagacctgcc catgcgggat atccacgaag gtgatttgat cgcaatccct gcttcggggg
     6781 catatcaact gagcatgtca tcgaactaca atggttcgag gcggcctgca gtgttgtggc
     6841 ttgaaaaggg aaatgcgcgc ctgatccaga gacgggaaac gatccaggat ctgctggcgc
     6901 gggatgtgat gatttaatcg ctttgcgcta cgaacgcaaa gaatgcttca acgatctgcg
     6961 ggtcaaattg ttttcccgaa cgttcgagaa tgtattgacg ggttttttcc tttgaccagg
     7021 cttcccggta gggacggtca gaggtaagcg catcccacac atcaacaatg gaaaatatcc
     7081 gtgcggccaa cggaatctct gtgcctttca atccgcgcgg atagcctgca ccatcccaat
     7141 gttcgtgatg acaataagga atatcgagtg cggcctgcaa aaagggaatg gcagccagca
     7201 tatcataggc atattgagta tgctgttgca tgatcttgcg ctcttgtgcg gtaaatgcgc
     7261 cgggcttgtg caggatggaa tcagggatgc ccattttgcc aatatcgtgc aacaaagcgc
     7321 cgcgccgaat gtgaacgatc tcagcttcgg ggatgttgag cgcctgcgcc agtttgatgg
     7381 ttaattctgt tacacgcctt gtatggcctt cggttaattc atcacgcaat tcaagggcac
     7441 gtgcccaacc tgcaagggta ttatcatacg ctgtggccaa ttcactggca tggttgaggg
     7501 tttgctcaaa gagtgcagca cgatggatgg cattactggt attttctgca agggcggtca
     7561 atagacggat atcggtctcg ttgaagatgc gcggctccga tgaccagaca tgcatgacgc
     7621 caatgatctt ttcctgtgtg cgcagcggca gggagattcc gccatgtacg gtcatgagcc
     7681 gttcccgttc accatccaat atggtgataa ccgaatcttt ttggatgtct tctgtgatgt
     7741 agatctcacc tgtcaaggcg acacggccag tgataccggt accgggctta tgcctcactt
     7801 ccgactcgtc ggccaatgtg aaattgactc cgggatccac cgaataccag ccatgtgaaa
     7861 caaaatcctg ggtttcggaa tcaagcagga atatggatgc aaaggttcca cccacagctt
     7921 gcaaggcatg acgggcaagg atgggtacca tctctgacac attctgggca gtgcgcaaag
     7981 ctgaagaggc aacagagagt gtctcaagtt ccatggcatg tcggcgaagt tcctcttcgg
     8041 cattttttcg gtcagtgacg tcaatcatca caccttcaag gtactgcact gcattcctat
     8101 cgtccaagac ccccaccccc cactcatcca cccagcgcca atctcccgaa cgatggcgga
     8161 tgcgataaga gatatgaaaa gacaggtcgc tggaggatgt atcctcccct acaaaggacg
     8221 gaattggaat ttgtttggcg tcttcaggat gatacaaatc tacaaaggac agaccacctt
     8281 ccagaaaatc ctctttggga tagccagtga gcatttcgac cgaatcattc aaatagatga
     8341 ctgtatagcg ttcatcgttt ttgcactgat acactgtgct ggggatgttc cccgccaaag
     8401 cacggaattt tctttcgctt tcacgcaggg catcctcggc cagcctgcgt tcagtgatat
     8461 cctgaaagat cacagtgaaa aatctttcgc ctgccacctg cgaattggag atgaaggctt
     8521 ccatgggaaa ttcctcacca ttcgcacgct tcccaaacaa agaatcaagc aatccttttt
     8581 gacgggctga gattccgctt ttggcatagt ccttcacatg caccccatga gaagcatggt
     8641 aacgatctgg gatcaatttg gaaagcggct gtccaataac ctcggaagtt gcatacccga
     8701 aaatgcgctc tgcagatggg ttaaatagaa taatgtgctg actctcatcg atcgtgatga
     8761 ttccattcaa cgccgaagag atgatcgctt ccagccttga acgactttcc tgcaaggcct
     8821 gttcagccag tttgcgctcg gtcacatcgc gatagttgac caccacacca tgaatatgtg
     8881 gatcatccaa catattgtga ccggtgatct cgaaatagcg ccactcccca tccatacggc
     8941 ggagacgata ttcggtcgtt cgaaccgcgc ccggagttgc cacgccttct ttgaatattt
     9001 tcatcacccc aggcagatct tgtggatgaa tgtattgaaa gccgctcgtc ccttgaacgt
     9061 tgtttgcact atatcctgtc aacttctcat ctgaaggcgc aatgtaacgg accacccctt
     9121 cctcatccag aatcgcgaca ccttcagcag aattttcaat gagagcacgg aagtatgcct
     9181 cacgctctgc cagcgccttt tcggcctgct tgcgttcgct aatatcgcgc acgattcctt
     9241 gaaaatatcc attcggcagc attctggcgc tgatctccac aggcagggta ctgccatcct
     9301 tgcaccggac atactgctca acgatcatgg accttcccat tcgaagttca tcaataagca
     9361 ggttcgagtg tccagcaatg acaatgtcat ccacactcat ggccagaagt tcttcaatgg
     9421 aataccccag catggctgca ccgcttgggt tggcatccaa tatcattttt tccgggttaa
     9481 caaggaaaat gccatcagaa gcctgttcca ccaaattacg gtagcgggtc tcagaaacgc
     9541 ggaccgcatt tacaagtttg gcgttttcaa tgatggaggc tgcatgccca gccatggctt
     9601 tcgccagccg aatatcagcc tggctaaact cacggcggcg acggctttcc caaatttcga
     9661 tgttaccgaa caactgtcca tgcgccagga tcggcacgaa catcatggag caaatcccat
     9721 attcagaaaa ttgctcacgt tctgtggggg tcatctcttc atcatcggaa tgcataatga
     9781 gcacttcacc cgcaagcatg gactttatga tgctgccata cccctcgttc tgaaaaactc
     9841 tgcccaaatc tgaatgcact tccctgggtg aagcttcagc gctccaatac tcccccacaa
     9901 cctgcatggt ggcagtaacg agattgatgt ccatgatatt gccactggtg actgacaacg
     9961 cctcggtcat gtgatgagcg agtttttcaa gcaattcggg ggcatccagc aaactggagg
    10021 acatatcttg agcagccgca tatagccgtt caagttcagc ggctttggct cgggtatctt
    10081 catcaaggcg caccttcaac attgccaaag caatctggcg ggcggtctgt tcagccaggg
    10141 caatatcatc tccggacaaa gggggtgctg attcacgaaa accaaagatg agcgcaccga
    10201 gtttcttttc atttgcaatg agaggcaatc ccaccgcaga aaaaacggga aacaaagaag
    10261 caacccgcgg actgatgaac gggctgttca tgatatcgtc cacgaccaat atctgccgat
    10321 tttcaagaat ggactgggtg aaggtcattt caccgggatt caaattcggg cgcgttccca
    10381 ggatgttttt ataaatatcg gaactgataa atttttccac ttgttgtgtt tcttcattcc
    10441 aaagtgaaaa ataacatccc tctgctccaa acatgccttc caaattttca ataagttttt
    10501 gaagcatggc ctcaaggctg ggggtttcaa gagcgacctg ggtaatggcg ttcaaaatcc
    10561 cctgatgacg ggcgtatacc tgcaacctgt cattgacttc gatcttttcc aatgcatagg
    10621 aaaggtcatt tgccaggtct tgaaggagat gaatttcttc atcttcaaaa accgcctccg
    10681 gtactgcatg atcaacaact aaaacgccca gaacacggtc ttcacgtaaa agaggcaatg
    10741 ccaccccaac ccgctccggg ttgtcgatgc ggcgcggaca acccttgcag ggatcatcct
    10801 cccctttaat tgggtctaca cggaagaagg agcgggattt tattgcattg gcagcacagg
    10861 ctaacccacg ctcattcaat tcatcaatac gtacattgaa caaagctggg tcaattttat
    10921 ctccagcttg ggctgcaagc tttaaggtca tctggtcgga ttcaagcagc ccagcccatg
    10981 catatgaata tccctgcccg gagacaagca aaatgcaggc ttcagaaagg agttgatggg
    11041 gatcctgttc acgaacaata agctgattaa ttcctgtcac agtgcgcaga atttgggaaa
    11101 gtcgttgttc gcgctgcaaa agttcctgcg atttttccaa attcgtgccc aaaacgcgca
    11161 gcaggttggt aatggaaatg agtacggcaa tattcagggt gagaaaaacc accaccccac
    11221 tgatccacga agaggaatcc gttgaaatga gttgacgctc gggcgggatc acaagaatgc
    11281 catttacctg caaatagccg acaattacca tggtagccaa accaatggca aaagctggca
    11341 cactgtagcg aaggtcgaaa aaaacagcgg caagaataat gaatgcaaaa agaatgaccc
    11401 gcccatcgcc gcttaaagac gagaaaacaa gccctatggc accaacgata taaacagcaa
    11461 aaagcaatcc ccaggcccga aggttatacg ggaggttttt cccaaaggta atcaacacaa
    11521 gtgtaaaagt cgccagcagg taaaccgaca atacaatccc tgcctgtaac aacggattgg
    11581 aggttttatc tacttccaga atgtaagact tgaagacatt gttcaccccc gcaataagag
    11641 acaatatcca aatcaacagg acaatacgaa gaagggccgc caaaaccagc tcgcgccatt
    11701 cttgaaggct gcgcgagatg gacgggtcaa agaaaaagat ggggcggaat aaggatttca
    11761 tctgcaattc aaattataca gttcataaac acaaaaacac cctttgaggt tactgaatat
    11821 agccgttctt tagggtggtc tctcacggtg ctgattctct ttcgaaacag aggcagtttg
    11881 caggcttgga taagaagcgg ggtttcatcc tgcaatgtga ctttagttac agtttgcagg
    11941 tttcatttgt gcaataattc acatagtcaa caagacatta taacaaggag agtgaccatg
    12001 aaagatatcg tcttcctgct tttgacgggc ctgctcgcgg cctacttgtg ctggtatttc
    12061 taccagcgct accagttgca caaggcactg cataacctgt attaccttat gggatttgca
    12121 gtgcttttgg tttcgggtct gttgctcata gttttcggac tcggcattct ttcctcgcct
    12181 tacgtgctga ccgttgcaag tttgattccg ttgggggttt ccatggggct tgctgaagaa
    12241 tacttccctg cctggaaaaa atatttcaaa tggtttgcgg cgctcggttt tctggcgatt
    12301 gcagtgacca gcattggcgg catggatttg ttgaaaaaga tcgcggttcc gctgttccac
    12361 ggggtggcgg ggttggtgat cttcattggc ccgttttatg cgaagggcgc gccaaagggt
    12421 ttcttttggg tgggcattgg cgggttgttg atcggcctcg gcggtattgc gctggcgttc
    12481 atcactctgg gtaaacagtt gcttttcttc agcccagagt ttgtgacgct cattctcact
    12541 cccctgctct ttttgatgac aggtgcatac gtgctgggtt ttgcaaaaag ggcataagtn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ccaccaaggg attgatcctt
    12721 gtggcaattg ccatggtggg aattgtcacc gccgtatggg ggatgctttc agggccgatg
    12781 gctgagatgg gggtacgcga gatcgttatc aaaacgcttg gcatgaagat cgtgcaggct
    12841 gaacgcgaag ggcgcatcat cattttgtat cattcgatcg ccatggcgat cattgccatt
    12901 gaaacctaca tggtaacagg gttgatcaaa atgaagcctt ttttcaaaac cgcgatcaat
    12961 gccaccatca cagttgggta catcctgacc atggtctttg gcatgggctt tgcatatttt
    13021 gggcgtaact gggcttttca cgggttatac attacagggc tttcgctgat cttctttgca
    13081 ggcctgatgt tgatcgtggc gctctggccg tggaatcctg aatatcatgt caccgataaa
    13141 agttacgccc acaccaaaag cggactggac ctggagcgga ccgccttttt tgccactgcg
    13201 gtcacaaccg taatctcgtc tctttttggc gcggtgccag gctcatatta cggccatggc
    13261 tttgaaacct ttctcgcaga gaacatcatt cgcctgcctg aaaaaaccgt gatggaatat
    13321 tcggtcatcg gtcatttgca catcatgctg gcattgatcg ggatcatgat cacgctcatc
    13381 atcgggcgct ggttgaattt caaaggcgcg tggcacaagg ccgccatgcc attaatgatc
    13441 cttggaacca tcgtactcaa cctgggcgtg tggggtgtgg ttaccccgct cgagcctgtg
    13501 gcacacatgg tcatctacgt cggcgcaacc ccgtccatgc tggcggcgct cttcctgctg
    13561 atctggagct gggccaagct catcaaggat ggcacggcac acctcacaaa gccaaccttc
    13621 ggtcagaaag tgggcgccct attacgtgac ccgctcaaat tcggtcccac ctggcaaatg
    13681 ctgttcatga atttcaccac cagcggcctc ggtattttca tggcgatcaa actcgatgaa
    13741 atctttcgtg tgtggcctgc acgtgaagaa cgcattgaac tcactggcca ttggcatgtg
    13801 ctttccgcca tcattgccac catcatcctc ctgtattatg gagatatgaa cggcttcaaa
    13861 ggcaggctgc gccagtggta tggctggggc atcatcattc tttcagatat cgcctttggc
    13921 gcggtcactt tgtttgaaat gaagcgtctc ttcgttacag aagccgccca acaaccactt
    13981 gtcaacgggt tgatgtatgc cattgatttt ggccttggca tgctgctggt catccttgcg
    14041 atcataatgg ttcatcgttt gatcgacctc ttcaagaaca aagggcgttg gatcgaggaa
    14101 ctggatcacg aactcgcaca ggaggtggac aaatgaaacg cttcactctg accctttgcc
    14161 tgtgtgcggc gttactggca tcctgcgccc ccgtggattt gaacgctccg atccccacct
    14221 ttgaaacagg cattgacccg aacacctggg cacagatccc agcaggtgaa ttccaccacg
    14281 gacagttcga tgaaaccgca tccacagaag cctacgagat catggtcact gatgtgaccg
    14341 ccgcacagta tgcagatttt ctcaacgcgg cgcttgcaga tggcagcata aaaatggacg
    14401 gcggcaaaat tgtcgggttc tacccaggcg acacgttcca cggggtcaag cacgaggaga
    14461 agatcgaagc gggcgattgg ctcttcattc cgcttgatga tccctcgcag agaatccaat
    14521 ttgatggaac aacattcacc gttcaatctg ggtacgaaaa acatcccatg acgagtgtta
    14581 cctggtttgg agcatgggga tattgccaat attttggcac ccgcctcccc accgaattgg
    14641 aatgggaaaa agccgcacgc ggcaccgatg aacgtccgtt tccgtggggt gatgaaattc
    14701 aacgcggcca tgccaacttc tactccagcc gcgacccgtt cgaagacatg gcttcttttg
    14761 gttcacgcac cagcccagtt ggtttctaca atgggcaggt ttacgacggc ttccaaacca
    14821 ttgactcagc ctccccctat ggcctgtacg acatggcagg caacgtctgg caatggacgg
    14881 gtgatgtata cgaaggaatg cattaccgtt ttatgcgcgg cggttcaaaa gacacctacg
    14941 acatggacct gcgcatttgg gttcgcaata acgccacacc cacttacttt agcccgggtg
    15001 tcgggtttcg atgtgcgcgg gatgagtaac aaaccaacca agtagacaag tacacatgta
    15061 cacaggtata cttgcgctct gtaatttggt tccctgttta cttgttccca tccatcgcag
    15121 gagtatctca tgcaagcgca attgtccttc agcacgatca gatccaaact cggtctgtat
    15181 atcccgctca tcaaatctct gcagaccagc ctgcttttgc tcacaggcgt ggcaggctat
    15241 ttgagtgcgc acaccaccgt aaactggctt cacttctggc aaatgctgcc cagtctcttc
    15301 cttgccattt ctggctccac catcctcaac atgtggtggg accaggatat tgacgcaaag
    15361 atgaaacgca cccacaaacg ccccacctcc gcaggtcagg tgacccgtga agaggtcttc
    15421 aacctcggca tggcagtgtc ggtcatcggc attggctggg cggtgatggt caactggtta
    15481 tacggccttg tggtatttgc aggtttattt ttcgacgtgg tcgtttacag catgtggcta
    15541 aaacgccgca cctgctggag cattgtgtgg ggcggcatct ctggtgccat gcccatcctc
    15601 gcaggccgtg tgttggccgt cggccagatc gatgtcatcg gcattttgct ggcctgcgct
    15661 gtattgtttt ggattcccac gcacacgctc accttcagcc tcaaattctt cgatgactat
    15721 caatcggcag gtgtgcccac cttcccctcc acctacggcg aagcggtcac ccgatttgcg
    15781 atcgcattct ccagcgtggt tgcagcactc accatgggct gggcatcggt ctggatcggg
    15841 gtcacagcag gtgtgctgcg actcatcatt gtgctgagcg cgggtctgtt cttccttgcc
    15901 atcaccacgg tttttcgccc atcagaccgc gtcaacttca gcttgttcaa atacgcatcc
    15961 atgtacatgt tgttcgcgat gttgctgatc attccgtaac gcaacattta tgttgccctg
    16021 catattgccc aatgaaaatg tcttccctcg accgcaccct gcttcttttc actggttttc
    16081 ttgccgcata ccagatcgcc atcggcattg accagatgga tagtgtttcc atcacagcct
    16141 acaccattgc tttcggtgtc atcctggtgg cagggcttct gctcatcatc ctcgggtttg
    16201 aagtgttgga tgcgcccatc gttgtgatca tctcaacgat cattccactc gccctgtcac
    16261 tcggcctcat ctggcagcac ctcgcctccc tgcggaccgt ttacctgatc ttcaccatca
    16321 tcagttttct cgccgtggta ttaacccgtt ccattgccat gcaaaacaag ctgcctgtca
    16381 ttgtcattgc catcacccac ggcattgcag gcctcaccat tttcctgctt cccatcatcc
    16441 ttgcagcaca gggacaggtg aaaccgcttt tctcccttgt gggcattggc ggagctttga
    16501 tcggcatcgg cgggttgttg ctttcctttc tgaaaacagg aaagccgatc ttatcaaggg
    16561 atacgatcat gagattgttc cccatgctgc tcctgctgac aacagcactc ttcgtagcag
    16621 gtttcaaact tggataacca acgtacaggt cacgcatgaa gcgtgacctg tttttttcac
    16681 aaaaggagta cacaatgtct gcaaataatt tctttgcaaa atccctgcgg ttcattggga
    16741 tcgtactgat ggcgcttacg ggcgggttta cttttcttgg cggcgcgggg acgttttgcg
    16801 cggcggtctt cccgcaaaaa tatgaaagca tggcggggtt gattccatac caatggctgt
    16861 atatcctctt catgcttgga acgattgcca tcggggtttg gggaatttgg gcggccatca
    16921 aactcatcaa aggcacagtg gatgcatacc gcatgagcct gtatgccttg atcacaggcg
    16981 tattggtcgg cggatttcac atctacatgt cgcgcatgtt acgtggaaag tccatgcctg
    17041 tggatgcggt ggtgtacacc acactgctta cactggtcat cttcctgctt ttcaagatcc
    17101 ccatgatctg gcagggcgtg gatttttcca aagcgaaagc cagtgataac aaaaaagcgg
    17161 gtggtgcggc ggcctttgtg gtggggattt tcacactcac catccaatat acaatgggac
    17221 ccacccacac atgggatggc attaactatg ccgatgcatt caatactgcc atgaccgtta
    17281 ttgggattgg gctgttggtt ttgggtactg ggatattcgt cagcttgggg aatgttggga
    17341 aaacagcact caggttacag gtccagcagt gatgcgggtg cgctgtggcg cacttccaga
    17401 tataaacaaa aacagccccg ccaaaatggc ggggctgttc aatttgatta ttccactttt
    17461 tctgatgaga cggattttcg catttggtct tcctgcacca tgcgaatgcc gttgctgacc
    17521 tggctgctca tttttgcgat ctggtcttgc agttgcatga gcgtatccac cacgtagtta
    17581 tcggcatcgg cgcgggtggc ttctgcttca ccgcgtgcct ggttgatgat ctgatcggca
    17641 cggcgctggg cttcctgaac gaccatgtct ttttgggtga gttggtcggc tttatcgcgg
    17701 gcaagctgca gggtgcggtt ggcttcttcc tgggcctggg ccattacacg gtcacgctgg
    17761 gccatgatct gctgggcctt ttttacttct tcagggatgg cgatacgcat ctggtcgatc
    17821 aattccaaca tgcggtcttc atccacaatg acgttgtgcg taaaaggcac agcttttgct
    17881 gcgttgaaga gttcttccaa tcggtcaata agttgcagaa tgtccatagt gcgcctctcg
    17941 tgggcggatt cctgatgaat gagaattact tttccaattc tagcacaaat taatcgcgga
    18001 gtgcgttggg tggagattgc tctcccatat taatcacttt gacatgcaaa gcagatttaa
    18061 catgggcagg caccatgctg gagacatcac catcaagcat ggcgatctca cgtaccgtgc
    18121 tggaggaaag aaaggtgtgt tgctcggcgg tgatcagcgc aacagtttcg atatcctttg
    18181 ccaggcgttg atttgccagc gccatgcgga attcgaattc gaaatcagaa aaaacccgca
    18241 ggccgcgcac gatgacctgg gcttggatct tgcgggcgaa atcaattgtc aggccgctgt
    18301 agccagtcac ttttatcttt gggttgtctt tgaatgcctc ggtgaccagt gaaatgcgtt
    18361 cctcaggtga gaacatgagg cttttcaagg gtttgtcgta cacagccatg accacttcat
    18421 cgaatagttt cgatgcgcgg gaggcaatat ccatgtggcc gtagtggatc gggtcgaatg
    18481 taccggggaa caatgctcta accatggtgg ttttgtagtt gcatctttga atcgtcgggg
    18541 aaattcgagc atgaccatgc gaccacgcaa caaattgacc cttggacaat tcagctaact
    18601 aacatctcct tttcctgtgc cccagaagcg ctcaaaggct tcagcaagca gggcgtgttc
    18661 gggattttgc agatacggat cgcgttcaaa cagggcttgc gcctgtgtgc gtgctttttc
    18721 gatcaaggat acatcggtga tgctggccat tttcaattgg gtgccaaagc ccgcctgacg
    18781 ggttcccaga aattctccgg ggccgcgtgt tttcaggtcg aggtcagcca gttcgaaacc
    18841 gttgttggtc ttggccatgg cctgcagacg ttcattttcg gtggcatcct catgagtggg
    18901 aatgagcaga caatacgact gcgccgcacc ccgaccaacc cgcccgcgaa gctggtgaag
    18961 ctgcgaaagc ccgaagcggt cggcgccttc aatgagcatg acggtggaat tcggcacatc
    19021 cacacccact tcaaccacgg tggtcgaaac gagaatatcg tattccctgt cgcggaattt
    19081 caacatcact tcgtcttttt ccgaggggcg catgcgccca tgtaaaagcc caagcttgag
    19141 atcggggaaa acttccttgg aaagcacatc gaaatcatcc actgcggcgc gggcttcgat
    19201 cttttcgctt tcatcgatca agggatacac gatgaaagcc tgcctgccgc tgcgaatctg
    19261 cccgcgaatc attgtgaagg cccgttcacg ttcctgaggc cgcagcacgt aggtatcgat
    19321 cggctgacga ccaactggca tttcatcgat tacagaaaga tcgagatcgc caaacacggt
    19381 caacgccagg gagcgcggaa tcggtgtagc ggtcatcacc agcaagtgtg gattcgtacc
    19441 tttgctgcgc agttccttgc gctgctccac accaaagcgg tgctgttcat caatgacaac
    19501 aaattgcagg tctttgaaga ggatatcagg ctcgatcaca gcatgtgtgc cgatgacgat
    19561 cttcaccgaa ccatctgcca acccctgacg gatcgattcc ttctcacttt cagttgtgtt
    19621 gcccaccaaa agtcgaatct cgctctgttg caaaaatccc tgcggccccg ccagcagatt
    19681 gctgaaatta cggaaatgct gctcagccag gatcgaggtt ggcgccatga tcgcagcctg
    19741 ggcaccttca ctgacaacga ctgaagagcc caaagcagcc accacggtct tgcctgaacc
    19801 aacatcgccc tgtacaagtc tgttcatcgg cctgcctgaa ttcaggtcgg cgcgaatatc
    19861 ttcaagcgat ttttgctggg cagatgtcaa agtgaagggt aagccgctta aacgcgaatc
    19921 catccactcg gtcgaaacag agaagcggcg ggcctccgca tctttccaat cccgtttctg
    19981 acgcagcacg cccatttgca ggaaaaagat ttcatcaaaa cccaatcgtt cgcgggcggc
    20041 cttgagcctg ctctgtgaaa cagggaaatg aacctgcatg agcgcttcac tcagtgaaac
    20101 cagccttgca gagagtttga tcttttcggg gagaatatcc accacggcag gcgcccagaa
    20161 cggaatcact tcgttcatga gtttgcggag ccatttttgt gtgacctttt cagtcaacgc
    20221 ataaatggga acaatgcggg ccgtatgcag gttttcaact tcgatcggct cccaatcggg
    20281 tgagttcatg atcaggcgtc ccaaatattg gtccaccttg ccagagacgg agatcgcctc
    20341 cccttccttc atacggttta taagccaggg ctggttgaag aacgacagac gcagtccacc
    20401 tgtcccatcg ttgatgatca cttcgacaat gctcgacttt ccaccgcgaa tagggcgggt
    20461 gtggacgctt tgaactgtac cgagcacggt caccgcttcg ccataaaaaa gggatttgat
    20521 cggcttgagc tggctgtaat cttcgtagcg gcgcgggaag taatacaaca tgtcaccgag
    20581 tgtgaagatg ccgatgcttt tcaacgattc ggcatgcttt gggccaacac cttgaagaac
    20641 agtgatatcc gcattcaaag cggctggggt ttggctggtc tttgcaccgg gcgcgctctc
    20701 aaaacgggag cgctgttgtt gtggaggtgg tgtgggtcgc ggcttgcgtt gttcttgggc
    20761 ttgaggtctt ggctggggct ttgattctgg cggaaccggt ggagtgggcg cttcgagttg
    20821 agcggggtcc gtgggttcgg gtcgcggcgg acggggcggc tggttcgcct tcgaaggctg
    20881 gggtggtgct tttggctttt gcccagcttc ggggtaggtt tctccaatcc gtttccataa
    20941 gcctttaagc gactcagccc ttccatccgg gctgagcttc tcataagagc gcagacgagc
    21001 cgcaacggct tgaattacct cttcagcaac tccatccgcg cgggcctccc cttcccaata
    21061 atcgagcatt ttggcaaggc cgccgataat tgcagtattc tcgtacttgt tttcatgttc
    21121 caggcgaaaa aatttacgta gtttttccaa agatggttgc atggggcaat tttatcatgg
    21181 ggaggtgtca tttcctcact gctgcaaaac cgatgccatt tggcccaaga tgggtgccga
    21241 tcaccgctgt gacgttgacg aacaaaatct cacgcggaac tgattttcgg gcttgattca
    21301 tcaaatcatc gagcaggttt ttggcgcggg gttcggcatt ggtgtgcaaa atggcaaggc
    21361 gttccaactc tcccctttcg agcagcagat tcaggatgcg ttcgtcggcc tgccgtgtga
    21421 ttcgcacagc accgacaggt tttacttcgc catccttcaa ttcgatcatc ggtttgatgg
    21481 aaagcaatcc acccaaagct gctacggctc cagggacacg tccgctgcgc ttgaggtatt
    21541 ccatcgtatc caatgcggca aaaacgtgaa gccgtttttg agtggaggta atggcatcca
    21601 atgcggattg caggccaaat tccgccgctt cggcagcagc gaggatctga aatccgatcc
    21661 cgagggaaag cgatccgctg tccacacagg tgactttgcc gggaaattcc tcggccgcct
    21721 tgcgagccac attgatcgtg gtggttaatt tcacggcagg gtggatggat atgatatggt
    21781 cacagccttt tgagagcaac gcctcatacg gggcggtgaa atctccgatc gaaggcgcag
    21841 ccgttgtcgg cggggtgcga agcgcaggca ggcgcgtata aaattcttcg cgggtgatat
    21901 tgaccccatc catatattcc tttccctcca aaaccagaat ggacggaatg acatggatct
    21961 cgtgttgttc aatgaggtgg gcggggagat cggaagtaga gtcggtgacg attcctattt
    22021 tcatattgaa agtttaacat aagaatgggg gaaggtgtca ttccgccctt gacatactgt
    22081 gtattacaca gtatcatgcg acaaaggttg atatatggca aacaatgaat cactggaaaa
    22141 tgttaccctc gaattacggc gcggtgtgat cgtgctggct gtgctcagtc agttgagcag
    22201 tgaacaatac ggctactccc tgctcaagtt gctttcagac caggggcttg aagtggatca
    22261 gggaacactc tacccgctct tgcgccgcct ggaaacgcag ggattgctcg aatcggtttg
    22321 gaagctggaa gaagcgcgcc cgcgacgtta ttacatcatc agtgcggagg gaaaaaagat
    22381 cctcccgaaa ttgaaaaaag aatgggctgg cattgtggct gtgatggcaa aagtgctggc
    22441 gtaaggagat gaaatgaatt taattgaccg ttatgtaact gaagttggaa agaacctgcc
    22501 gatcctcaag ggacgcgagg atattgagaa ggaattaaag tccacgcttg aagatatgct
    22561 ggaagaccgt gtggaacaaa cgggccagcc gcgcagtgaa gccatggaga tcgaactgct
    22621 gaaagaatat gggtcacctc aaaaagtggc agcgacctat aaccctcagc catatttgat
    22681 cggcccgcgt ctgtttccct tctttttgat ggtactgaag atcgtgattc ccatccttgt
    22741 aactgtttta ttggtgctga cagggatccg cgccgcaacc gacacacctt tcatgggtgc
    22801 gggttttatg aacatcgtca gcaaagggtt gggtgggatc gtttctgcgg cgatcaccgc
    22861 tttcggcaac atcgtattca tcttcgccat tctggagcgt gtgctcccag acaaggaaat
    22921 cggcgatttc agcaacgaaa aagactggga ccccgccacc cttgccaaag aaccagaccc
    22981 tgatacggcc aaacgcagtg acttgatcgc agagatcgtt ttcaccttca taggtcttgc
    23041 cattttgaat ctgttccccg atattcttgg catgtcttat tttgctgaag acaaaatggt
    23101 ctttattccc atgttctcag ccgaattctt caaattcctg ccctggatca atgctgtttt
    23161 tctcgctgaa attgtgctgg atatttacct gctccgcagt gcggtatgga cttccatcac
    23221 tcgaattgcc aagatcatca ttgaagttgc cagcattgca gtggcggttg ccattctgcg
    23281 cacgcccaat atcttcgggt tcaccgctga gtcgttccaa agcgtgccaa acagttcgat
    23341 caacgctgat ttgctgctga atattttcac cctttcgttt tcgatcgcca tcatcatcgc
    23401 catcattgtg gcaggagtgg atatggcaaa ggcagccttc ggtttgttga gaatgaatta
    23461 caaaagaaaa taagttaaaa caagaagaac gctgatcggt cgcttttgcc tgcacattgc
    23521 gcgatcagcg ttcttctgcg tcttgagttg aattcggggg taattatgtc ttgtttattc
    23581 gatcgaaatg ataaattggt agtgcggctg tccgccttcc tgtacttcca cttcgagggc
    23641 ggtgtatttg gtgacgatca aatctgcgat gcggttggct tcagcatggg tcatgccttc
    23701 gccgtagtac aagctgacca gttcgtgctg gtcggcatcg gctttttcaa gcaattccat
    23761 cacaccctgc tccaccgatt cagcagagac caccagcttg ccatccaaca acgcaatgac
    23821 ctgtccatcc ttcacgctga cgccgtcaat ttcaacggtg cgggtggcaa ttgtaacctc
    23881 gcctgttttg accgaaccca aagctttggt cattttatcg gcgacagaat caagttcccc
    23941 gtcgggaatg agcgagagca tggcggcaag tccctgcgga acggtgcgag tcggcaccac
    24001 agccacctgt tttacggtta cttctttggc ctggtttgca gcgagaatga tgttcttatt
    24061 attcggcaaa ataatgatct tatcggtcgg caggttttca aaggaactca gaatatcctg
    24121 cgtggatggg ttcatacttt gtccgccagc cacaacggcc gccacaccca ggctggcaaa
    24181 aatacggctg atgcctgccc ccggcgaaac cacaaccacg gcaatatgtc cgggctcaac
    24241 tgtagcaaac tggatctgct tgcttttttg aatatcatcc atctgggcaa gcaggttttc
    24301 gatggcaacc tttgtcacgg tgccaatgcc cataatgtaa tcgatcggct cgtaacgttt
    24361 ttcggtgggc acgtggatgt gcatacggta catcccttcg ccctccccca cctgaatgga
    24421 agtacccatc tcttcaagtc ggccataaaa tgcgccgagg tcaagttcac ctgtgggcac
    24481 aaaatccaca accacttcgt aatcctgccc ctcttccact tcttcgaggg caccctgcaa
    24541 attcatagcc gacatgggct tgacgctcat ggtgggtgtt tcaagtgact caccatacac
    24601 atgcctgagc atgccttcca aaatgaagaa cagtcccttg ccgcccgagt caaccacgcc
    24661 tgcctgtttg aggacgggga gcatttccgg cgtttttttg acagcttcat ctgcggcttt
    24721 aaccgccact tcgaggattt cgatcgcatc ccgctccgcg gccgacccta aagctgcttc
    24781 tgttgcattg gcaacttctt taatgacggt gagaatcgtc ccttccacag ggcggacgac
    24841 gcccttatag gctgtgtcac gcgcttcgcc aaaagccttc accaaagtgg ggacatccaa
    24901 tgtggctttt tcatgcacac cgcgtgaaaa tccacgcagg atctggctgg tgataacgcc
    24961 cgaattcccg cgcgcaccca tcaaggcgcc cttggagacc atggccgcca tctcgccgag
    25021 gttgcgggca cccgaatcct tgacctcgtt ccacgcggct tgcaaagtca gcaccatgtt
    25081 ggtgcctgta tcaccgtcag gcactgggaa aacgtttaaa gcattgaccg tttccttgtt
    25141 ggtgcgaagc caggtcagcc cggcatcgat caagcgtttt aatgactgcc catcaatcgg
    25201 cattcgacga aatttctcta cgcgtgcaga gtccacaccc ataagttttc tcggctccta
    25261 atctgtatcg ctgatgcgca gcccggcgac atgcacattc acggaattta cacgcagtcc
    25321 caatgctttt tccacatgga agcgcacggt gttggccaca ctgacagcca ctgagttgat
    25381 gcgagtgcca tactctacaa cgacgttaat atcgatgtcg atgtcttcgc ccttgtaacg
    25441 gacggtgatt ccgcgtgaag gttcgcgggt aatggtggaa acgatgccat ctgccagatt
    25501 tttcggcgca agccccacca ctccatagga ttgcagggtg gcataataag caatggttgc
    25561 caccgcgttt ggcgaaatat gaatcccgcc caaggttgtt gtttcgtcgc ccataggttc
    25621 ctcaatttta atcatcatgc cgaggggggg tgcagcacat aacactgctg atgcccaagg
    25681 ctcttctgtt ggatcggacg gtcttgccgc ccatacgctg gctttccgtg tctattttaa
    25741 catcttatct tgaagaaggt tgcgttgtat ggtaaaattc cgcgcctttg agtaaaggcg
    25801 ctgacccagc ggcatgttgc caggtcaggt agtggaatca ttgtaattga aaggaagtat
    25861 cctaaaatgg caaaatgcag cacctgcggt aaagcaacca ccttcggcca caatcgttcg
    25921 ttctccctgc gtgcaacaaa ccgcacgttc aagcccaacc tccagaaggt ttcggtgatg
    25981 gagaatggcc gcctggtcaa gaagacgctg tgcgccaagt gcatcaagac actcggcaaa
    26041 tctgcggctt agtttgtttt ccattcacat caaaggtcac ggcttcggcc gtgatttttg
    26101 ttttaacgcg gactaaacac attcccgcaa tattttgatg ccctgcccca caccttgcgg
    26161 atgaccaaac gcagcgctgc ctgccacgaa catatctgca ccggctttgt acatgagcgg
    26221 caaagtcttt gcagacatgc ccccgtccac ctccaggtgg gcggttgaat gcaatgcatc
    26281 caaccgggcg cggatctctt ccactttgcc gaccatttca ggcatgaacg cctgacctga
    26341 atatccggga accacactca tcaccaaaac aaggtcaaca agcggcaggg cggaatctaa
    26401 tgcagaggca ggggtagcgg ggttgagtgt gatccctgcc ttgcagccca gggatttgat
    26461 ctttttcacg gtgccaacca gatcggggca ggtttccaca tggacggtga tattgtttgc
    26521 gcctgctttg gcaaaggcct caagatattt ctcaggctct gagatcatca aatggacatc
    26581 caatgggagt ttggtaacac gtttacaggc ttcagtaaaa agcggaccta tggtaatggt
    26641 ggggacaaaa tgcccatcca tcacatccac atgcagccaa tcggcgccag cagattcaca
    26701 ggcggcgatc tcttcggcaa tgcgggtgaa atctgcggcg atgatagagg gggcgaggag
    26761 gaatttattc atgagtcaga aagtaaaacc gacgccgtaa acaattgatt ttattaaatg
    26821 ggaggattat aggcaaagta aaccatcatc caaaatggga tgaggccacc cactgccagc
    26881 acagcaaaca aggcgaggcc ccaaaagacc cagtcaattt cttcgaaggc agagacaggc
    26941 tcgggtgatt cctgaggcgc tggttcgggc cgggtatatt gcctgggggc agtggtctgt
    27001 cgggaagtgg aaaccgtttc cttggtcggg agtttatccg aaatttctgg cgagacgctg
    27061 atgactgttg cggggggtgg cacaggtgtt ggcgcagggg caggggcatc gggctgggtg
    27121 ccaaatttga gcagcacacg cccaagttga tctgcggtgc gatagcgtgc agcgggctct
    27181 ttggagagca ccttcataat aatttcttcg agtgcggtcg ggatatcagg gatatattcg
    27241 cggatgggaa tcgggcgggc ggaaatatgc aggcgagcca attcatcgga ggtggatgcg
    27301 gtgaacggtg gcgtaccgca gagcaattca tacatcacca caccaatgga atagacatcg
    27361 gacgcaggcg aaggggcctc cccttgggcc tgctcgggcg caaaataaag cggcgacccc
    27421 cagacaatgt cagtgcgttc gccgggtgtc atagtggcga gggcgcgcgc aatgccgaag
    27481 tcggtgactt tgaggcggcc atcttctgaa acgagcatgt tgtgcggttt gacatcacaa
    27541 tggacaagcc cggcacggtg ggcatacccc agcccggcac aagcctgaat aatgagcgga
    27601 atgcccgtct ctactgaaaa gcggccacgt tcgcggatga gttgtttgag gtctttgccg
    27661 gggatgtact ccatgacgat gtagagcagg tcatctgcaa agccaaagtc atgcacggtg
    27721 acgatgttgg ggtgcgaaag atttgccgcc gaacgcgctt ccatgcggaa atgcttctgg
    27781 aattcctcgt tggtcgaata atccttgcgc aggactttaa tcgccacata gcgatccagc
    27841 atgagatcgc gcgcccgaaa gacatcggcc atgcccccgg taccaagcct ttcgagcagt
    27901 tgataacgat cgttgagaag ggtctcgtca gtcat
//
LOCUS       DAOQJC010000049        27879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_746_length_27879_cov_10.156735, whole genome shotgun sequence.
ACCESSION   DAOQJC010000049 DAOQJC010000000
VERSION     DAOQJC010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 27879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27879
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_746_length_27879_cov_10.156735"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PLE39_09300"
     CDS             complement(<1..304)
                     /locus_tag="PLE39_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68565.1"
                     /translation="MKKFFSSFFNAPWYPIVISAYPVLALLSSNAGQVQSNTGIRPLL
                     VSVAFGGLLFFIVWVILRRQVHRAAFLTTLLLALFFTYGHAYIAVDEKWPDAGYAKW"
     gene            complement(404..2416)
                     /locus_tag="PLE39_09305"
     CDS             complement(404..2416)
                     /locus_tag="PLE39_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68566.1"
                     /translation="MQTKNEKSNPFWLSALIIFVVSAIAYLPLARKFGYLNDDWYLMY
                     DMWVKDPSFFHVIFSGDRPGRALAMIPLFSLFGFEPLYYHLSAFLFRFLSGVSLYWTF
                     RMLWPWKRFAALAAALLFAVYPGFLSQTNAIDYQSHILGLFLAALSVALTIKSLLTVE
                     RFPRFFFMAASILAGWGYLSQMEYFIGMEAFRFGCIFILMWRTQGASYLQKAGNWFIA
                     SLPFLLVAGGFLIWRLFFFESERKATDIGLQISQLTSSPLTLLWWLNYLVQDMVSVTL
                     VAWGLPVYVLAFPMRLRDVMSAFGWAGLAVALMVLGMNRVDKSKSEAEAGPSLSREAL
                     ILSLISIVGGLLPVILVNRHVTLPDYSRYTLIASVGAALLLVTIIEKISARVALLSFF
                     VAIAVLTHYGNAVRAVDEAQATRDFWWQVAWRVPQFQPGVTLIASYPGSPLSEDYFIW
                     GPANLIYYPEKQDANPVEIKLPAAVLTDDVVLEIATNGGVETPLRRGNYLERDFGNVL
                     VMIQSRSNGCVRMINGEVPELSPLDQHRLLLIAPFSRLENVKTEGEFPTPPAVVFGSE
                     PERGWCYFYQKADLARQRGDWEQIPVLLKEALDAGHYPEDALEWMPFLQASAVLGDVE
                     QVRSTAKLVTVDKFLRLQVCNIMTDFMEKETLNDEVKSVIEHTICK"
     gene            complement(2524..3186)
                     /locus_tag="PLE39_09310"
     CDS             complement(2524..3186)
                     /locus_tag="PLE39_09310"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNE68567.1"
                     /translation="MKLAALVPMRHHSQRVPGKNYRPLAGKPLFQHIVETLLAVPEIE
                     TVMVDTDSEPVMEGVRRLFPTVKLIQRPEHLRADDMPMNEILLHDTAQVQADFYLQTH
                     STNPLLKPQTVSRAIQSLFTNYPKNDSLFSVTRLQTRLYFQDGRAINHNPLELIQTQD
                     LPPVYEENSCLYIFTRENLERKRHRIGDTPLMFEIDADEAWDIDEELDFEITDFLMRK
                     RM"
     gene            complement(3222..4049)
                     /locus_tag="PLE39_09315"
     CDS             complement(3222..4049)
                     /locus_tag="PLE39_09315"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE68568.1"
                     /translation="MPSFSFKPASEFSIPQLADLLTRGFEGYFVPINITPPVLLAMMR
                     RDGIDLTESRVLLKDGGEVGISLIARRGWTSRLAAMGIVSSARSGGAGTWAMQNLIQE
                     AKQRGEKEMLLEVIEQNTAGVKLYEKVGFQKIRRLVGYKLENPKIESGEELEEIDIRE
                     LARMVTYHGLNDLPWQLSGTTVMHFTPPSRAFRLNDAYCLISNPEMNDIAIQSVLVKA
                     RSRGAGLSAVLMRSLFAKFPNKVWHVSPIYPEEMGFIFEHVGMTREDISQWQMSLTL"
     gene            complement(4058..4522)
                     /locus_tag="PLE39_09320"
     CDS             complement(4058..4522)
                     /locus_tag="PLE39_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE68569.1"
                     /translation="MSKLIPMTQTEFDAFLEQVVPDYAADNVKAGYWSEEEALEKSRS
                     EFMRLLPQGLQSENHYLYTLYDEDQAVGRIWMRANFDRPTKSGFVFELWVDEQFRGKG
                     YGKQAMLLLEEKAREMGLKSIGLHVFGYNTVARGLYEKLGYEVSSLNMSKNL"
     gene            complement(4665..5435)
                     /locus_tag="PLE39_09325"
     CDS             complement(4665..5435)
                     /locus_tag="PLE39_09325"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE68570.1"
                     /translation="MKSKTIVLIHGMFMTSLCWEKWIPYYEAKGYRVFAPAWPGREKS
                     VEELRKAHPDSTLAKLKLNHIVDHVETFIKGLDEKPAIIGHSMGGLVVQLLLQRDLAV
                     AGIAIDPAPPAGVFTTEFSFLKANFPAINPFLLNQPVQMSFEHFQYAFVNTLPLDEQR
                     AAYDRYVVPESRGVPTSSLGAAGKIDFSRLHRPLLITAGEKDHIIPASLNRSNYGKYK
                     GPAVTNFKEFAGRDHFLIGSRGWEEVADYCLEWLGKIE"
     assembly_gap    5461..5560
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5680..6150)
                     /locus_tag="PLE39_09330"
     CDS             complement(5680..6150)
                     /locus_tag="PLE39_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNE68571.1"
                     /translation="MNGDNIQGLETLEAWKKAKSLALVIYREVLPVLPPEEKWHLDQQ
                     IRRAAQSVFANIAEGHGRFYYQENIRFCYIARGSLTETYTHLVLAHDLKYIPDELYSQ
                     LKGQIEELLRIINGYIAYLKRSRRGENEPGANHVVRELEPDYIIDDIVNQSSKG"
     gene            complement(6194..7126)
                     /locus_tag="PLE39_09335"
     CDS             complement(6194..7126)
                     /locus_tag="PLE39_09335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE68572.1"
                     /translation="MISIIIRAYNEEKHIGRLLEGIKQQTIQDVDVILVDSGSTDSTV
                     AIAESYGTRIVRIRPEEFTFGRSLNFGVKEAKREFIVMASAHVYPVYPDWLETLLRPF
                     QDEKVALTYGKQRGYEGSKYSEHQIFHQWYPDDSNADQTTAFCNNANAAIRKSLWQQH
                     PYDEMLTGLEDLEWAKWAKGQGYKIAYAAEAEIIHIHNETPRGVYNRYRREAMALRKI
                     YPEAHFNFYDFLRLTTTNILSDLWHAARERVLLKNISSIFWFRYMQFHGTRMGHRETS
                     LVTPQLRETFYYARERKMKDERKRDVEPIRYNSE"
     gene            complement(7154..8002)
                     /locus_tag="PLE39_09340"
     CDS             complement(7154..8002)
                     /locus_tag="PLE39_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNE68573.1"
                     /translation="MALKSSLRSIIYQTEKIAQRLRFASQPASLPVLLGISFPKSGTH
                     LLDQILLGFSNIAPYAKRVHSFYAEYEGESGKKREPEKALAWLDSLRPADVASAHLFA
                     RPEAITRVISPKFAPYFIFRDPRDVVVSHVFYVTDMETRHVHHDFYQSLPDFNARLKA
                     SILGRPDSGIEFPNIADRFAPYLGWLDQKEVLTIHFEDLIHNREATLTSIMDHLLSRV
                     SLPVTRQLILDSLETSINPKKSPTFRSGKTGEWKKHFTDEHKQIFKEVAGDLLVKLGY
                     EKDGNW"
     gene            8058..8663
                     /locus_tag="PLE39_09345"
     CDS             8058..8663
                     /locus_tag="PLE39_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE68574.1"
                     /translation="MKLTIFNTPIISPLLRFLSNSTMRLAGWRVEGKLPDLPKYIIIG
                     APHTSNWDFLLFLGVIFRLKADVRYMGKAELFRSPFGWFFYWCGGIPVDRKKSTGLVE
                     QMVEACSKSEKFILTIAPEGTRHYVNEWKMGFYHIAKSAGIPIVMAKVDGKTKAMRVG
                     EIFYLTDDMDADLKAIKGLFEGMTGVNPDKKKKKKYITLEK"
     gene            9041..9547
                     /locus_tag="PLE39_09350"
     CDS             9041..9547
                     /locus_tag="PLE39_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68575.1"
                     /translation="MEHPTLYEKGVLSWKGMVWLLWKHEAYRNLFYYRIKKDYRIFSM
                     IILELAKLIYKPRNTLFIRADYIGEGLFIQHGYATGISAHSIGKNCWINQHVVIGYSD
                     TGKAPTLGDNVRIAMGAKVFGEISIGDNSIVGANAVVAKNVPPNCTVVGVPARIIKRE
                     GKRVDESL"
     gene            9574..10023
                     /locus_tag="PLE39_09355"
     CDS             9574..10023
                     /locus_tag="PLE39_09355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HNE68576.1"
                     /translation="MIIDRIENSLPYYTLHPKFKAAFEYLSQINIHSIPVGRHEVDGD
                     SIYALVQEYNTKLKEDGKWEAHRRYIDLQYVVQGAEGMGYANIHHLNQGEYDASKDFL
                     PLFGEGDQVSLKSGCFVLLFPEDAHMPGMAIGEPAFVRKIVLKIAVE"
     gene            complement(10099..11724)
                     /locus_tag="PLE39_09360"
     CDS             complement(10099..11724)
                     /locus_tag="PLE39_09360"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68577.1"
                     /translation="MLKEFFTQRIKRGLVRRLNNIKVASAARKVARMAPHPNGAPIVF
                     FKASTGIDDLSWNSGFHLLTTWALRLQGIPVAYFACNSGLSKCVLGTNRDNPHKEMPC
                     KSCVMQSKALYKNVETFERSNVSTSSVNWFGFQRHLELENALRDLNIDQLSHFTFQEL
                     PLGPLCLPGLRWVLRIHHLSDDENTRYLLREYILSAWNVAQKFSDFLDQTQPRAVVVF
                     NGQFFPEATARYIAQKRGLRVITHEVGLQPATAYFTEGEATAYPIHIPAEFELSDAQN
                     AKLDAYLARRFQGDFTMAGVKFWADMQGLDESFLKKAAQFKQIVPVFTNVIFDTSQPH
                     ANTVFEDMFDWLDMVLEIARENRDTLFVIRAHPDETRVRKSSRETVEGWASSRKVEEE
                     ANIVFVSPREALSSYELIQKSKFVMIYNSTIGLEASIMGAAVLCAGKARFTQYPTVFF
                     PQTVDEVRIKTKEFLSADVIDVPSEFKRNARKFLYYQLYRTSLPFGDFLEPSVRTTQT
                     RLRSFDLNDLKRSESVQVILDGLLNGGDFLLKE"
     gene            complement(11771..15196)
                     /locus_tag="PLE39_09365"
     CDS             complement(11771..15196)
                     /locus_tag="PLE39_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNE68578.1"
                     /translation="MRPKLDLAGNSALVESPPRVRIWISALRYLLGKALTIFATIFAG
                     VFLTLLLANQPSPRGLDPARSPFEISLESQIARVIRISIANGTIPRLSDGYPDQHQLD
                     ALEKKLRDDAGLELPFFPRYLWWTLNALQFNWGELTVTSTETTGFSLADPVGKRDLVL
                     EYLPNTLLLIGTAFLLIFLIGMPLSLYLARNYGGWFDRLLTFLSPISSIPSWVVAMLL
                     IALFAVKLRWFPVGGKFDFATPQDPLEYILTLFRHMVLPVSAIVFSMLFQVVYAWRTF
                     FILYSEEDYVDLARAKGLPHKILERQYILKPSLPYVMTSFATIVIGFWQLTVALEAVF
                     QWPGIGWLYIREALPNFWGESMDPGQLMLVIEIVVIFAYLLGAVVFFLDLAYALVDPR
                     IHLLPSRNTTQGRVKTRMQGTDWLARFNAWMKEKTSRKPKQATGPVRPLKFIWEAFAD
                     SLHDFDARSRLFFHELRRYPSAMFGLTVISIMLVGSIYAVIGLPYEKYGMEYNTNRVT
                     GRSYTPRLAVPAWTNLFSSMPRLSTLVINEKSPRAQVSLRSLENGWVEKTITFTFDYA
                     YRETPSDIFLYLEPKYVEKFPFISLTWITPDGRDLDLVAKSINAVTNYDFETGIRVAK
                     LIRQNPEWEKWFVRSGQYPTPAINLLFATPHEPQLHLQSGQYQLVLKSLLFEDESDLT
                     AELVLLGQVYGAAGTDYWRRDLVVPLLWGMPFALIIGLLGTLVTILVAMLLPAIGVWY
                     GGWLDEFVQRLTEINMVLPGLMIAVLAYALFGINIWIVLGLVVMMNAFGSPIKTFRSA
                     LLQAREAPYIEMARSYGATDFRIITRYLVPRILPVVIPQLVSQIPGFIFLEATLGLFN
                     IKSNYPSWGRIIYDGLARNALYGSPFWVLAPISLLLLTGLAFALLGTALERVLNPRML
                     EQVSQAEEKEIAKQRRVSKRMIATITAAALLMFFILVPSIKTLSGMFNRAPSTASDRM
                     AVPVAVTLPSVTAPSPTETPDPVPQASSTLTFSPTPTLTPSPTILPSLTHTSTPLDLR
                     PKTYTLQAGEFPYCIARRFDIDPIDLLALNGFGPAQTFYTGMTLQIPQNANPFPAQRV
                     LRAHPATYIVIQPYESMGGIACQFGDVDPIAVADANQLAVDSPLFVGQQLHIP"
     gene            15405..16307
                     /locus_tag="PLE39_09370"
     CDS             15405..16307
                     /locus_tag="PLE39_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68579.1"
                     /translation="MGRKRSTAKNSPKTTKEWAQIIVALIAAVATVSAAVIAIIPVFI
                     KDAPETATQSADAPDASSANVQDGLVGKMPPENADRFLVNRFYKPTQHMGDRGDIDIS
                     GIGDTANPIIFTYSTNGNGPHEWDYKYINKEINPNPARFAGIMFIDSAGPGGTDPNNG
                     FDLSAYKTIRWEARSVQGLVDVKFVIGGVTWTWDEQTKERVVAPYPDSVRSSVLLCEP
                     TLTQEWQSFEYDISGFPPDSFQHVVGVFGWTIDWGDNNITSITNPDSSIVPENPKTLV
                     IEIRNIYYDKAIVEHPATSDCGSK"
     gene            16308..17837
                     /locus_tag="PLE39_09375"
     CDS             16308..17837
                     /locus_tag="PLE39_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68580.1"
                     /translation="MSTKYVLHTLTIIWFVLLISGCANPPTATQTSPSTPTLPSGWEK
                     KVSNIIWIAYSPPNANPNKGIQASAEDIQADLEVLHNAGFTGLVTYDSKGVMGRDFPT
                     LAKETGFKGVIMGIWDPTSAEEMSNAQAASSNPIVLGFCVGNEGLDVRYDMTVLSSTI
                     EELRKTTGKPVTTAEQLDDYYDEKLLEIGEWVFPNAHPYFHSQIDPVNAVKWTEGAYK
                     DLSSKTDRFILFKEVGLPTEGDAENNLSETAQDQYYQGLEYTDVKFVYFEAFDQPWKT
                     NLSVEPHWGIFFSDRTPKQTAIRFMGGEPVVEFPTATATEIPVETELPISDDIFYIYN
                     DGASPYNHFSPSGYMGDTGDIQIDGASTENPQSGDTSIQVIYTPKGQGPYACAYKPPC
                     KWAGVYWQQPANNWGKEQIWAGTGFDLSAYRTLKFWARSESTCTLEFKVGGITGDYGD
                     SLVYPRGITERLTTEWQEYEIPLEGADLSYIIGGFVWSTSWEKCPDGATFYLDEIRFE
                     K"
     gene            17913..18818
                     /locus_tag="PLE39_09380"
     CDS             17913..18818
                     /locus_tag="PLE39_09380"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HNE68581.1"
                     /translation="MNRIDRLFAILLELQHKRRLRAVDLARQFEISKRTIYRDMSALN
                     QMGIPIAALPGEGFELVEGFYLPPLMFNENEAVALTLGLRLLTQQAAGSLTQAADSAS
                     AKIKVALPEQVRVKSEALSGIIGFITPSQKFNLDDSQLLLIQKAIQEKQVMHIRYHGY
                     RKDEISERDVEPHQLYYSDGVWYFEAYCRLRKDVREFRLSRVEKLTLLDQHFSKRRKS
                     KAAAARMVEVRIRFDARILRWVRERQHYGFVREKDSVMTYHVHDVSEITSWILGWGAE
                     AEVLAPKDLRKELRETAKKLANLLT"
     gene            18872..19195
                     /locus_tag="PLE39_09385"
     CDS             18872..19195
                     /locus_tag="PLE39_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68582.1"
                     /translation="MSNQVIELASFRLADGASEATLIAASDAMQEGFLKSQKGFIKRD
                     LVKGADGQWADVVYWDSQESVDAAMQEAMNNPACFKYFELMTGVNHEDVSAGVMHLSV
                     LKSYL"
     gene            19204..19533
                     /locus_tag="PLE39_09390"
     CDS             19204..19533
                     /locus_tag="PLE39_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68583.1"
                     /translation="MVIKAVLIGSVLGVLEWVILVGSRQKLGFWIYIQSMLAWFTCGF
                     MIGVVDSPFPAYLLGSVLAVFLNLPWYINISIIPKQYNHLPPLIISSIVLGAIGGGLK
                     ILLSAMF"
     gene            complement(19616..20470)
                     /locus_tag="PLE39_09395"
     CDS             complement(19616..20470)
                     /locus_tag="PLE39_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNE68584.1"
                     /translation="MGIKQNIRRAFRPYGKTAQAIWRWKRFSFNDAPPIFANSKPKSG
                     SHLLLQVMNGFTQIMPYKYVDADPVRTITYDGRRKNKEEILADLKHTPKGVIGWGYVD
                     ATEENASFLTAAGRVNYFIYRDPRDMLVSQVFFATDMHEEHGMHEYYKSLPDFAARLN
                     VAITGIDRDGLKMVSVKQRYEGVFQWLEQKNVMCIRFEDLINNRDATLLKMLDEVEKT
                     GYKISTPREKALSVLVEAIQPKKSHTFRSGKTGGWKQHFTEEHKKLFKEVTGNLLVRL
                     GYETTNDW"
     gene            complement(20556..21437)
                     /locus_tag="PLE39_09400"
     CDS             complement(20556..21437)
                     /locus_tag="PLE39_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNE68585.1"
                     /translation="MAREIKFGNRMMGDGHPAYVIAEVGINHNGDLDIAKKIIDAAAH
                     AGADAVKFQKRTPEVCTPPEQQKQMRETPWGYITYLDYRYKVEFSEEQYREIDRYCKE
                     KKIDWMVSVWDEPSVDFMEKFDTPAYKVPSASLTDHNLLKYVRKTGKPVIISTGMSTM
                     DQIKRGVEVVGEDNLVIMHCTSTYPCEPEELNLNMIGTLRREFPNNPIGYSGHEVGLV
                     PSAVAIALGATSIERHITLDRAMWGSDQAASVEPGGFERLVKYIRVTEAAMGDGVKKV
                     YESEKGSMKKLRRVVSE"
     gene            complement(21427..22671)
                     /locus_tag="PLE39_09405"
     CDS             complement(21427..22671)
                     /locus_tag="PLE39_09405"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HNE68586.1"
                     /translation="MTDILALIPARGGSKGIPRKNIRSFAGYPLIAWSIAAAKQSELV
                     TRVIVSTDDEEIAAVAREWGAETPFMRPAEFAQDKTTDLPVFEHALKWLEDVEGYRPD
                     IVIQLRPTSPVRPHKMVDDAIRILLEHPDADCVRGVVPAGQNPFKMWRFHGEGKPLNP
                     LLTVDGIAEPYNAPRQILPPVYWQTGHIDAIRTATIAQKASLTGDVIYPLVIDPKYTV
                     DIDTLPDWAKYEALVYSGLEMVSPGAPRRLMPETVKMIICDFDGVVTDNLVITDQDGR
                     ESVTASRSDSMHIKKLREMGVEVMILSSEPNPVVLARAKKMGVEAIHNVGMQDKGRVM
                     REVLAQKNVKAQNVVYVGNDLNDLPCFEIAGWSVAVADAYPEVIRAADHVLTKTGGHG
                     AIRELCEIVLKQLSQKENTNGS"
     gene            complement(22717..23544)
                     /locus_tag="PLE39_09410"
     CDS             complement(22717..23544)
                     /locus_tag="PLE39_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE68587.1"
                     /translation="MTIFDKFSLKDRVAVVTGGVGLLGAEFCKTLAEAGAAVAVVDLN
                     GEAASKVADGLVRSGYKAMGIATDITKPESVNAMVASVLSTFGRLDVLVNSAALDPKF
                     DPDAAAKGIAPGAFEDYPLEQWNAAMNVNLTGMFLVTQACVKQMNAQGKKGSIINVCS
                     TYGLNGPDQRIYIKDGKRVAYKPVYYTTTKAGVMGFTKYLAAYYAETEIRVNALTPGG
                     VFNNHEEYFVKNYSAKTILGRMAKKDEMNGALLFLASDASSYMTGNNVIVDGGWTAW"
     gene            complement(23581..25218)
                     /locus_tag="PLE39_09415"
     CDS             complement(23581..25218)
                     /locus_tag="PLE39_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNE68588.1"
                     /translation="MPLNCGAADQRHGSDSISVETCQVFLSFMTRQRAASFATLGLLA
                     ILGTVFILYATPEGLGLSDDSIAYIAGARSLLSGQGYREAWLASNGPVTHFPPAFSSI
                     LALIGLSGLDPVRGARFLNASLFGANTFLLGLIGWRMTKSQAAGFVLALLFVLNASLL
                     RIHAVAMSEPLYIFFSLGAFLLFEFYIEQREKRWLILAGCFVGAAYLTRYAGLALFAT
                     FLAALILLRENWLKRITRSLIFIASFFPWAAAWSFRNKLVADNATNRTLVYHPLTEEN
                     IQAGIYNFSEFLVPIEAWRRALVKNLDLLFVILAILGLIILVWVAVTGLKKFFKPSSP
                     MPEILSFTNGLYVFAYLASILSSMMLFDASTKFKARINAPIYISLLILLVWFGFWLWQ
                     KRTLLWRGIVVGAAVFLVGLSSRELVGVVRQLHKGGQGYASFQWYDSAAMDFLSALPE
                     GIRIYTNQAGPVYLYTNRPAYVLPDLVDPVTGLPRERYEEGVQALQEDVLSGNAVLAL
                     FKFGAEEEDVQGIYLQLAEGLNLALDKRGDKIYTNQP"
     gene            complement(25245..26972)
                     /locus_tag="PLE39_09420"
     CDS             complement(25245..26972)
                     /locus_tag="PLE39_09420"
                     /inference="COORDINATES: protein motif:HMM:NF004040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HNE68589.1"
                     /translation="MAKAKLIPPYGGKLVNLVVEGKEREDLLSRAGKLPSIKITMRNL
                     CDLELIATGGFSPLTTFMGKADYDRVLKEMRLADGTLFPLPITLTADPKELPTVGEEL
                     ALRNANFDLIAVMTLEEVFHWDADTEAAHAYGSTDSKHPMVSEMGRWGKVCISGPMKV
                     VNLPKYYDFVNLRHTPAQVRARLEEMGHDNVVAFQTRNPLHRIHEELTKRAAAQVNGS
                     LLVHPTVGMTKPGDVDHYTRVRTYKALVDNHYDKNSTMLSLLPLAMRMAGPKEALLHA
                     IIRRNHGANHFVVGRDHAGPGNDSSGKPFYGPYDAQENMKKYEAELGVKMVPFEMLVY
                     LPDEDRYVEEKDVPKGAKTANISGTQVRDDYLAKGKLLPEWFTRPETAEILREMYPPR
                     HKQGFAIWFTGLSGSGKSATTAVLTTLLLERGRETAILDGDVVRTHLSKGLGFSKEDR
                     DTNILRIGFVAGEIAHAGGIVICAAISPYRATRQEARKMVGENFIEVFMDTPVEVCEQ
                     RDVKGLYAKARQAMQDGKPMGFTGVDDPYEPPIDPELTLKGFGASPEENAHLIVKYLE
                     EQGYILPVK"
     gene            27304..>27879
                     /locus_tag="PLE39_09425"
     CDS             27304..>27879
                     /locus_tag="PLE39_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68590.1"
                     /translation="MNSKFLRNALFALLVFGYIYYCQRYIAATSFIIEGKTYYVLFDD
                     AMISMRYAYNLAHGLGPVWNAGERVEGFTNPLWVGLMALVHLLPIGLNETSLVMQIIG
                     ASLLTLNLFLVKSILEHFTDDLFAMLAAVALTAFYAPLNSYGLLGMEVSLLALMLSTV
                     IWLIIRNADFSLHPTPWVYGILAISTLVRFDM"
BASE COUNT         7197 a         7001 c         6968 g         6613 t
ORIGIN      
        1 gccatttggc atacccagca tcgggccatt tttcatccac ggcaatgtag gcatggccat
       61 aggtaaagaa caaggccagc agcagggtgg tcaaaaacgc ggcgcgatgc acctgcctgc
      121 gcaagatcac ccagacgata aaaaacaaca aaccgccgaa cgccacagaa accaacagcg
      181 gacgtattcc cgtattcgat tgcacctgcc ccgcattcga agaaagcaaa gccagaacag
      241 ggtacgcgct gatcacgatc ggataccacg gcgcgttgaa gaaactgcta aaaaactttt
      301 tcattaataa agtgacctcg tttttaccac gcagggtcac aaaggaacac aaaggaaaac
      361 cctttgtgac cctttgtctc ctttgtggtg gattttcaga tatttacttg caaatcgtat
      421 gctcgatcac agatttcact tcatcattca gcgtttcttt ttccatgaaa tccgtcatga
      481 tattacagac ttgaaggcgc aggaatttat ccacggtgac caatttggct gtgctgcgca
      541 cctgctccac atcaccgagc accgctgaag cctgcaggaa aggcatccac tccagcgcat
      601 cttcggggta gtggcctgca tccaacgcct ctttcaacaa aacagggatc tgctcccaat
      661 cgccgcgctg acgtgccaga tcggccttct gataaaaata gcaccagcca cgctcaggtt
      721 cgctgccaaa tacaaccgct ggcggagttg gaaactcgcc ttcggttttt acattttcca
      781 aacgcgagaa cggcgcgatt aacaacagcc gatgctggtc cagcggcgac agctcaggca
      841 cctcaccatt gatcatgcgc acacagccat tcgaccgtga ttggatcatc accaacacat
      901 ttccaaaatc acgctccaga taattgccgc ggcgcagcgg ggtttccaca ccgccattcg
      961 tcgcgatctc cagcaccacg tcatcggtca gcacggcagc gggcaatttt atctcaactg
     1021 ggttcgcatc ctgcttttca ggataataga tcaaattcgc aggaccccaa atgaaataat
     1081 cctcgctcaa cgggctgccc ggatacgaag cgatcaacgt caccccaggc tgaaactgcg
     1141 gcacacgcca cgcaacctgc caccaaaaat cacgcgtcgc ctgcgcctca tccaccgcac
     1201 ggaccgcgtt gccgtaatgc gtcagcaccg caattgccac aaagaatgaa agcagcgcaa
     1261 cccgcgccga aatcttttca atgatcgtca ccaaaagcag tgccgccccc accgaagcga
     1321 tcaaggtgta acgcgaataa tcgggaaggg tcacatgtcg gttgaccaaa atcacaggca
     1381 ggagtccgcc cacgatgctg atcagactca aaatcagcgc ttcacgactc aaactcgggc
     1441 ctgcttctgc ttccgattta cttttatcca cccgattcat ccccaacacc atcagcgcaa
     1501 ccgcaagtcc cgcccaccca aaagcggaca tgacatcacg cagccgcatc ggaaacgcca
     1561 gcacatacac gggcaatccc catgccacca gcgtcacact gaccatatcc tgcaccagat
     1621 aattcaacca ccacaacaac gtcagcggcg aagaggtcaa ctgcgagatc tgcaaaccga
     1681 tatctgtggc cttgcgttca gattcaaaga aaaacaaccg ccagatcaaa aatccgccag
     1741 ccaccagcaa aaacggcaga ctggcaatga accaattccc cgccttctgc aggtaggaag
     1801 cgccctgtgt ccgccacatc aaaataaaga tacaaccgaa gcgaaaagcc tccatgccga
     1861 tgaaatattc catctggctc aaatagcccc agccagccaa aatggaagcc gccataaaaa
     1921 agaaacgcgg aaaccgctcg accgtcaaaa gcgacttaat ggtcaaggcc accgacaggg
     1981 ctgccaggaa caaccccaaa atatgcgatt gataatcaat ggcattggtc tgtgagagga
     2041 agccaggata caccgcaaac aaaagcgcag ccgccaatgc ggcaaaccgc ttccacggcc
     2101 aaagcatgcg gaaagtccaa tacaagctca cgccgctgag aaagcggaac aggaaagcgc
     2161 tcaggtgata ataaagcggt tcgaatccga acagcgagaa tagcggaatc atcgccagcg
     2221 cccgcccggg gcggtcaccg ctgaaaatga catggaagaa actgggatct ttcacccaca
     2281 tgtcgtacat caggtaccag tcatcgttga ggtagccgaa tttacgcgcc agcggcagat
     2341 aagcaatggc agagaccaca aaaatgatca gcgcactcag ccagaatgga ttggattttt
     2401 cgtttttagt ttgcatgtga tttcatgtcg ttgcgagggc ggtgatcgtc cgcccgaagc
     2461 aacctcatgg tcaaagtgga gattgcttcg ccgcaaagag caatggcgcg gctcgcaatg
     2521 acattacatt ctctttctca ttaaaaaatc ggtgatctcg aaatccagtt cttcgtcaat
     2581 atcccaggct tcatcggcgt cgatctcaaa catcagcgga gtatcaccga tgcgatggcg
     2641 tttgcgttcc aaattctcgc gggtgaaaat atacaggcag gaattttctt cgtacacggg
     2701 cggcaggtcc tgcgtttgaa tcaactccag cgggttgtgg ttgatggcac gcccatcttg
     2761 aaaatacaaa cgtgtttgca ggcgggtcac tgaaaaaagg gaatcatttt taggataatt
     2821 ggtaaacagc gattgaatgg cgcgtgagac ggtttgcggt ttcaacagcg gattggtgga
     2881 atgtgtctgc aaataaaaat cggcctgtac ctgggctgtg tcgtgcagca aaatttcgtt
     2941 catcggcatg tcgtcggcgc ggagatgctc gggacgttgg atcagtttta cggttggaaa
     3001 gaggcggcgc actccctcca tcacaggctc ggagtcagta tccaccatca ctgtttcgat
     3061 ctcaggtacg gcaagcaggg tttcaacgat atgttgaaaa aggggtttgc ccgcgagcgg
     3121 gcgataattt ttgccaggca cacgctggct atggtgtcgc atggggacta gggcggcaag
     3181 tttcatgccc cctattttat acccataatg ggtgctgcgt cttacaaagt cagtgacatc
     3241 tgccactgtg aaatatcttc gcgcgtcatg ccaacgtgct caaagataaa tcccatttct
     3301 tcgggataga tgggtgaaac atgccagacc ttgttgggga atttcgcaaa aagtgagcgc
     3361 atcaatactg cgctcaggcc tgccccgcgt gaacgggcct tgaccagcac cgactggatc
     3421 gcgatgtcgt tcatctcagg gttgctgatg aggcagtagg catcattgag gcggaaggcg
     3481 cgcgaaggcg gcgtgaaatg catgaccgtt gtgcccgaaa gctgccatgg cagatcattc
     3541 aagccatgat atgtgaccat gcgcgccagt tcacgaatgt cgatctcttc gagttcttcg
     3601 cccgattcaa ttttcgggtt ttcgagtttg taccccacca gcctgcggat tttttggaag
     3661 ccgacttttt catacaactt cacaccagcg gtattctgct caatgacctc aagcagcatt
     3721 tctttttcac cgcgctgttt ggcttcttga atgaggtttt gcatggccca ggtgcctgcg
     3781 ccgccactcc gcgccgaaga tacgattccc atcgcagcga gtcggcttgt ccagccgcgg
     3841 cgggcgatga gagaaatgcc aacctccccg ccatctttga ggagtacacg gctttcggtg
     3901 aggtcaatac catcgcggcg catcatggcc agcaaaacgg gcggagtgat gttgatgggg
     3961 acaaaatacc cctcaaagcc gcgtgtaagc aggtcggcta attggggaat ggaaaactct
     4021 gaggcaggtt tgaaggaaaa tgaaggcatg gagtgaatta cagattcttg ctcatattca
     4081 agctggatac ttcgtagccc agtttttcat ataagccgcg tgcgacggta ttgtatccaa
     4141 agacgtgcag gccaatcgat ttcaagccca tttcgcgggc tttttcttcg agcaggagca
     4201 tggcttgttt gccgtagccc ttcccgcgga attgttcatc aacccagagt tcgaagacaa
     4261 atccgctttt tgtggggcgg tcaaaattgg cgcgcatcca gattcggccg acagcctgat
     4321 cctcgtcata caaggtgtag agataatgat tctcgctttg caaaccctgg ggtagaagcc
     4381 tcataaattc actgcgtgat ttttccaacg cttcttcttc gctccaatag ccagctttga
     4441 cgttgtcggc agcatagtct ggaaccacct gctcaaggaa cgcatcaaat tcagtttggg
     4501 tcatggggat caatttggac ataagatgat tataccgaaa aacatacagc caccctcaag
     4561 gtgactgtat tccatgtaaa tcgccgatct ttacagaagg cctgaaagat taaccactga
     4621 gacacagaga aaacaattaa gaattccgtg tttcaaaaat ggatttattc aatctttcca
     4681 agccattcga ggcagtaatc tgccacttcc tcccaaccac gcgaaccgat cagaaaatga
     4741 tcgcgcccgg caaattcctt gaaatttgtg acggcggggc ctttgtattt tccgtaattg
     4801 gagcggttca atgaagcagg gatgatgtga tccttttcac ctgccgtgat caagagcgga
     4861 cgatgcaggc ggctgaaatc aattttgccc gcggccccaa gagatgaggt gggaacaccg
     4921 cgcgattcag ggacaacata acggtcataa gcggcgcgtt gttcatccaa aggcaaagta
     4981 ttgacaaaag catattgaaa atgctcaaaa ctcatttgca caggttggtt aagcaaaaat
     5041 ggattaatgg cggggaagtt cgccttgaga aacgagaatt cagtggtgaa tacgcccgcc
     5101 ggtggtgcag gatctatggc aatgccagcc actgccagat cacgttgaag cagcaattgc
     5161 acgaccagcc ctcccatcga gtggccgatg atggcgggct tttcatccaa acctttgatg
     5221 aaggtttcca cgtggtcaac aatgtgattg agctttaact ttgcaagcgt cgaatcaggg
     5281 tgtgctttgc ggagctcctc cacagatttc tcacgcccgg gccaggcagg tgcaaaaaca
     5341 cggtaacctt tggcttcgta ataaggaatc cacttctccc agcaaaggga ggtcataaac
     5401 atgccgtgaa tcagaacaat ggttttggat ttcatggttc tcctgtaaca ataaagcggg
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtgaatcaga acaatggttt
     5581 tggatttcat ggttctcctg taacaataaa gcgggcgaaa atatagggaa tagggaatag
     5641 ggaataggaa ataggggata gggaaggtta agactacgat tatcctttcg aagattgatt
     5701 tacgatatca tcgataatat agtcaggttc taattctcga acaacatggt ttgcccctgg
     5761 ctcgttttca ccgcgccttg aacgtttcaa ataagcaatg tagccattaa tgatccgtag
     5821 caactcctcg atctggcctt tcaattggct atatagttca tcgggaatat atttgaggtc
     5881 atgagcaaga acaaggtgtg tataggtttc ggtaagtgaa ccacgggcta tataacaaaa
     5941 acggatattt tcctgataat aaaatctgcc atggccttca gcaatattcg cgaacacact
     6001 ctgtgcagca cgtcgaattt gttgatctaa atgccatttt tcttcaggag gcaaaacagg
     6061 caggacttct ctataaatga caagagccag cgacttcgcc ttcttccagg cctctaaagt
     6121 ttccaagcct tgaatgttat caccattcat agcattctcc cgtctgccac cactatttcc
     6181 tattcactat ttcctattca ctattatacc gaatcggctc cacatcccgt tttctttcat
     6241 ctttcatctt tctttccctc gcataataaa aggtctcgcg caactgtggc gttaccaggc
     6301 tcgtttcgcg gtgacccatg cgagtgccgt gaaattgcat gtagcgaaac caaaagatgg
     6361 atgaaatatt tttcagcaaa acccgttcgc gcgcggcatg ccataggtca ctcagaatat
     6421 tggttgtggt gaggcgcaga aagtcgtaaa agttgaaatg ggcttcgggg tagatcttcc
     6481 gcaaagccat tgcttcgcgg cggtagcggt tatagactcc acggggtgtt tcgttgtgaa
     6541 tatgaataat ttcggcttca gcagcgtaag cgatcttata cccctgccct ttcgcccatt
     6601 tcgcccactc cagatcttcc aaccccgtca acatctcatc gtagggatgt tgctgccaca
     6661 ggcttttacg aatggcggca ttggcattgt tgcagaacgc agtcgtctgg tctgcattac
     6721 tgtcatcggg ataccattga tgaaaaattt gatgttcaga gtatttcgag ccttcgtatc
     6781 cgcgttgttt gccgtaggtg agagccactt tttcatcttg aaacggacga agcaaagtct
     6841 ccagccaatc gggatagaca ggatacacat gcgcgctggc catgacgatg aactcgcgct
     6901 tcgcttcctt aaccccaaaa ttgagcgagc gtccgaaagt gaattcttcg ggacggattc
     6961 tcacgatccg cgtgccgtat gattcggcaa tggcgacggt tgagtctgtg gagcctgaat
     7021 ccacgaggat gacatccaca tcctggatgg tctgttgttt aatgccttcg agcagccgtc
     7081 cgatgtgttt ttcttcatta taggcgcgga tgatgatgga gatcattggt aattggtaat
     7141 tggtaattgc ggtttaccag ttaccgtcct tttcgtagcc cagcttcacg agcagatctc
     7201 ctgccacttc tttgaatatc tgtttgtgtt catctgtaaa gtgttttttc cattcgcctg
     7261 ttttgcccga gcggaaggtc ggggattttt tcgggttgat ggatgtttcg agggaatcca
     7321 gaatcagttg tcgggtgaca ggaagcgaga cgcggctgag gaggtgatcc atgattgagg
     7381 tcagggtcgc ttcgcggttg tggatcaaat cttcgaagtg aatcgtcagc acttcttttt
     7441 ggtcgagcca gccgagatac ggggcgaaac ggtctgcaat gtttggaaac tcgatgcccg
     7501 aatcggggcg tccgagaatg cttgctttta gcctggcgtt gaaatcgggg agcgattgat
     7561 aaaaatcgtg gtggacatgg cgggtttcca tatcggtaac atagaaaaca tgtgagacca
     7621 ccacatcacg cggatcgcgg aagatgaagt atggcgcgaa ttttggcgag atgacacgag
     7681 tgatggcttc gggacgggca aagagatgcg cggatgcaac atcggcgggg cgaagcgaat
     7741 caagccaggc gagcgctttt tcaggttcgc gtttttttcc gctttcgcct tcgtattcgg
     7801 cataaaagga atgtacgcgt ttggcgtaag gtgcaatatt tgaaaaacca agcaaaatct
     7861 ggtcaagcaa atgtgtgccg cttttcggaa aggaaatgcc gagcaataca ggcaggctgg
     7921 caggctggga tgcaaaacgc aaacgctgtg caattttttc agtttggtag ataatggagc
     7981 gcagggaaga tttcaaagcc atgcaggtat tttactgcaa ggcgtgccat ttccatgttg
     8041 acagggtttt atattccatg aaactgacca tcttcaacac ccccatcatc agtccgcttc
     8101 ttcgtttttt gagcaactca accatgcgcc ttgctggctg gcgcgtggaa gggaaattac
     8161 ccgacctccc aaagtacatc atcattggtg cgcctcacac ctcgaactgg gattttctcc
     8221 tgtttctggg ggtcatcttc cgcctgaaag cagacgtgcg ctacatgggc aaagcagaat
     8281 tgttccgcag cccgtttggc tggttcttct actggtgcgg cggcattcct gtggatcgga
     8341 aaaagtccac tgggttggtg gagcaaatgg tagaggcttg cagcaagtct gaaaagttca
     8401 ttctgaccat cgcccccgaa gggactcgtc attacgtcaa cgagtggaag atgggttttt
     8461 atcacatcgc caaatcggct gggattccga tcgtgatggc aaaagtggat ggaaaaacca
     8521 aggccatgcg tgtgggagaa attttttatc tcacagacga tatggatgcc gacctgaaag
     8581 cgatcaaggg attatttgag gggatgacag gcgtcaaccc tgataagaag aaaaagaaaa
     8641 aatacatcac attggaaaaa tagtttttcc ctctttcgtg ctggattaat tattaagttc
     8701 aggccatttc caattgtgta tcactggtcg gacaattcaa atcaaattag tgataattgc
     8761 gggagattta ttatcatatt catatgatcg tgacgctgca aggatggtag aatttcgaca
     8821 gcaatttaat ctggcataca aagttcatgt atctcaagaa ctttgatgcg atagggatgc
     8881 tatgaacagg gacaacggat ttaagagcag ctatctattt caaacagcca ccgatatact
     8941 atttgcattt catatcctca gatccatctt ccacattttt cttttgacca cctctccatc
     9001 acggcatgtc attgaagccg atatcacaag gtggatggaa atggaacacc ccacactgta
     9061 cgaaaaaggt gttttatcct ggaaaggcat ggtctggctt ctttggaaac atgaagccta
     9121 tcgcaatttg ttctattacc gcatcaagaa agattatcgc atcttcagca tgatcatcct
     9181 tgaattagcc aaattgatat acaaaccaag gaacaccctt ttcatcaggg cagactacat
     9241 cggcgagggg ttatttatcc agcatggata tgccacgggc atatcagctc acagcattgg
     9301 gaaaaactgc tggatcaatc aacacgttgt gatcggatac agtgacacag gcaaagcccc
     9361 cactttggga gacaatgtac ggatcgccat gggggcaaaa gtgtttggcg aaatctcaat
     9421 tggtgacaac tccatagtgg gtgcaaatgc tgttgtcgca aagaatgttc cccccaattg
     9481 cacagttgtt ggcgtcccgg cgcgcatcat caaacgtgaa ggcaaaagag tggacgagtc
     9541 gttataagca catccaccaa cggttatata aaaatgatca ttgatagaat cgaaaattcc
     9601 ctgccatatt acacgcttca cccgaaattc aaagctgctt tcgaatacct gagtcagatc
     9661 aatattcatt ccatccccgt cgggcggcat gaagtggatg gagattcgat ctatgccctt
     9721 gtgcaggaat acaacacaaa gctgaaagag gatggcaaat gggaggcgca tcgtcgctac
     9781 atcgatctgc aatatgttgt gcaaggcgcc gagggcatgg ggtatgccaa cattcatcac
     9841 ctgaatcaag gcgaatatga tgccagcaaa gatttccttc cgttgtttgg ggaaggtgac
     9901 caggtgtcgt tgaaaagcgg ttgttttgtt ttactcttcc ccgaagatgc gcacatgcct
     9961 ggcatggcaa ttggagagcc tgcctttgtc agaaagatcg ttttgaagat cgcggtggaa
    10021 taataaaata ttctgcaaac acaaagaatc aaaaagtata gtgtagcaac tgcaaattca
    10081 aggaaaacct ggcagttgct actctttcaa cagaaaatct cccccattta acagtccatc
    10141 caaaatcacc tgcaccgatt cggagcgctt aaggtcgttc agatcaaacg atcgcaagcg
    10201 tgtttgcgtc gtccgcaccg agggctcaag aaaatcgccg aatggaagcg acgtacgata
    10261 caactgataa tacaggaact ttcgcgcatt gcgcttgaat tcggatggca catcaatcac
    10321 atctgcgctc aaaaattctt tcgtctttat tcttacttca tccaccgtct gcggaaaaaa
    10381 caccgtcggg tactgcgtaa accgcgcctt gcctgcgcac aatactgccg cccccatgat
    10441 ggaagcctca agcccgatgg tggagttgta gatcatcaca aatttggatt tctggatcag
    10501 ctcgtaggaa ctcaacgcct cacgcggact tacaaagacg atattcgctt cttcctccac
    10561 cttgcggctg cttgcccaac cctcaacagt ttcgcggctg gattttctca cccgcgtttc
    10621 gtcagggtgc gcgcggatga caaacaaggt atctcggttc tcgcgggcga tctccaaaac
    10681 catatcaagc cagtcgaaca tgtcttcaaa aacggtgttg gcatggggct ggctggtatc
    10741 aaagatgaca ttggtaaaga cagggacgat ctgcttaaac tgagccgcct ttttcagaaa
    10801 ggattcgtca agtccctgca tatctgccca gaatttaaca cctgccatcg taaaatcgcc
    10861 ttgaaagcgt ctggcaagat aggcatccag tttggcattt tgggcatcac tcaattcaaa
    10921 ttcagcgggg atgtgaatgg ggtaggcggt ggcttcccct tcggtgaaat aggcggtggc
    10981 aggctgtaac ccaacttcat gcgtaatgac gcgtagtccg cgtttttggg cgatgtatcg
    11041 agccgttgct tcggggaaga actgcccatt gaaaaccacc acagcccgag gttgggtttg
    11101 atcgagaaag tcagaaaact tctgcgccac attccacgcc gagaggatgt actcgcgcaa
    11161 aagataacgg gtattttcgt catcgctcag gtgatgaata cgcaagaccc agcgcaaccc
    11221 aggcaggcaa agcgggccaa ggggaagttc ctgaaatgta aaatgtgata actgatcgat
    11281 attcagatca cgaagggcat tttcaagttc aaggtgacgt tgaaacccga accaattgac
    11341 gcttgaagtt gaaacgtttg aacgttcgaa cgtttcaacg tttttataca acgcctttga
    11401 ctgcatcacg caagatttgc acggcatttc cttgtgcggg ttatcgcggt tcgtgccgag
    11461 cacacattta ctcagccctg aattacaagc aaaatacgcc acagggattc cctgcaagcg
    11521 caacgcccac gtggtcagca aatggaaacc actgttccat gaaagatcgt caatgccagt
    11581 tgatgctttg aaaaagacaa tgggcgcacc atttgggtgt ggtgccatac gagccacttt
    11641 gcgtgccgcc gatgcgactt tgatattgtt aagcctgcgg acgagtccgc gcttgatgcg
    11701 ttgggtgaaa aattctttca acatggattt tctcgccccc aatcttatca gcaaatcatt
    11761 tgttacggca ctatggaata tgcagttgtt gaccgacaaa caggggggaa tccacggcaa
    11821 gttgattggc atccgcaacg gcaatcggat ccacatctcc aaactggcag gcaattccac
    11881 ccatagattc atatggctga ataacaatat aggtggcggg atgcgcccgc aatacccgct
    11941 gtgcaggaaa gggatttgca ttttgcggaa tttgcaaggt cattcctgta taaaaagttt
    12001 gagcagggcc gaagccgttc aaggcaagca ggtcgatggg atcgatatcg aaacgacgtg
    12061 caatgcaata agggaattcc cctgcctgca aagtgtaggt ctttgggcgc aggtcaaggg
    12121 gggtggatgt gtgtgtcagg ctcggaagga tggtcgggct gggagttaga gtgggtgtcg
    12181 gggaaaatgt cagcgtggaa gatgcctggg gaacaggatc aggcgtttcg gtcggagaag
    12241 gagcggtgac tgagggcagg gtcactgcca caggcacggc cattctatcg gaggcagttg
    12301 atggcgcacg gttgaacatt ccagagaggg ttttgatgga aggaactagg ataaaaaaca
    12361 tcaacaaggc tgcggcagtg attgttgcga tcatcctttt tgaaacgcgg cgttgttttg
    12421 ctatttcctt ttcctctgct tgagagactt gctcgagcat gcgcggattg agaacacgtt
    12481 cgagcgccgt gccaagcaaa gcaaacgcaa ggcctgtaag caaaagcaaa gagatcggcg
    12541 ccagaaccca aaagggcgaa ccatacaatg cattgcgtgc cagcccgtca taaatgattc
    12601 gcccccacga tggataattg gatttgatat tgaacaaacc cagtgttgcc tcaagaaaaa
    12661 taaagcctgg aatttgggag accaattgag gaattacaac aggaagaatg cgcggcacga
    12721 gataacgtgt aatgatcctg aaatcggttg cgccatagga gcgagccatt tcaatgtagg
    12781 gcgcctccct ggcctgtaac aatgcggaac gaaaggtttt gatgggagaa ccgaaagcat
    12841 tcatcatcac cacaagccca agcacgatcc agatattgat tccaaataat gcgtaagcca
    12901 gaactgcgat cattaatcct ggcaacacca tattgatctc ggtgaggcgt tgaacgaact
    12961 catccagcca gccgccatac cagaccccaa tggcgggtaa aagcatcgca acaagaatgg
    13021 taacaagtgt cccaagcaaa ccgataataa gtgcaaaagg catcccccac aacaacggga
    13081 caaccagatc gcggcgccag taatctgtgc ctgccgcgcc atatacctgc ccaagcaaaa
    13141 caagttctgc agtcaggtcg ctttcatctt caaagagcag gcttttaaga accagttgat
    13201 attgcccgct ctgtaagtgc aattgcggtt cgtgtggtgt tgcaaagaga aggttaatgg
    13261 caggcgttgg gtattgcccc gaccgaacaa accacttttc ccattcggga ttttggcgaa
    13321 tcagttttgc aacgcgaata cctgtctcaa aatcataatt cgtaaccgca ttgatggatt
    13381 ttgcaaccaa atcaagatcc ctgccgtctg gcgtgatcca cgtcaacgag atgaaaggaa
    13441 acttttcaac atacttcggc tcaagatata aaaaaatatc gctcggcgtt tcgcggtagg
    13501 catagtcaaa agtgaatgtt attgtttttt cgacccagcc attttccaat gaccgcagcg
    13561 atacctgagc tcgtggactc ttttcgttaa tgacaagcgt tgaaagccgc ggcattgaac
    13621 tgaacaaatt cgtccaagcg ggaacagcca gccgtggcgt gtaactgcgc cccgtcaccc
    13681 gatttgtgtt gtactccatg ccatattttt cataaggcaa cccgatcaca gcataaatgg
    13741 atcccaccag cataatggag atgacggtca acccaaacat ggcagatgga tatctgcgaa
    13801 gttcatggaa gaagagtctg ctccgtgcgt caaaatcatg gagcgaatct gcaaacgcct
    13861 cccaaatgaa tttcaacggt cgcactggac ctgtggcctg cttcggtttt ctcgaagttt
    13921 tctctttcat ccaggcattg aatcgggcca accagtcggt tccctgcatt ctggttttca
    13981 cccgcccctg cgtcgtatta cgcgatggca gcaaatggat tcgtggatca acaagggcat
    14041 aagccagatc caggaaaaac acgaccgcgc caagcaaata cgcaaaaatg accacgatct
    14101 cgatcaccag catcaattgt ccgggatcca tgctttcgcc ccagaaatta ggcaatgcct
    14161 cacgaatata caaccacccg attccaggcc attgaaaaac cgcctccaaa gccacggtca
    14221 actgccagaa gccaatgacg atcgtggcga aactggtcat cacgtatggc agggaaggtt
    14281 tgaggatgta ttgccgttcc aatatcttgt gcggaagtcc ttttgcccgt gcaaggtcaa
    14341 cataatcctc ttcagaatag aggataaaaa aagtacgcca ggcataaacc acctgaaaca
    14401 gcatgctgaa aacaatggca gaaacaggca acaccatgtg cctgaaaagc gtcaatatat
    14461 attcaagcgg gtcttgcgga gtggcgaaat caaatttccc gccgactgga aaccagcgca
    14521 gtttcaccgc aaacaaagcg atcagcagca tggccacaac ccaactcgga atggaagaga
    14581 tcggcgaaag gaatgtcaac agcctgtcaa accagccgcc atagtttcgc gccagataca
    14641 atgacaatgg catgccgatc agaaatatca gcaaaaacgc ggttccgatc aaaagcaggg
    14701 tgttgggcaa atattccaac acaagatccc gttttccaac ggggtctgcc agactaaatc
    14761 ctgttgtttc ggtcgaggtt acagtcagct ctccccagtt gaactgaagc gcgttcaatg
    14821 tccaccataa ataacgcgga aaaaatggaa gttccaatcc agcatcgtca cgcagttttt
    14881 tctcaagcgc atccaactga tgctgatctg ggtacccatc tgacaacctt gggatcgtcc
    14941 catttgcaat gctaatacgg atcacacgcg cgatctggga ttccaaactg atctcaaatg
    15001 gagaccgtgc agggtcaagc ccgcgcgggg aaggctggtt tgcaagtaaa agtgtgagaa
    15061 agactcccgc aaagatggtg gcgaaaatcg tcagtgcttt tcccagcaaa taacgcaagg
    15121 cactgatcca gatccgtacg cgaggcggag attccaccaa agcagaattc cctgccaaat
    15181 ccaatttcgg tctcatctaa tgggttatac ggtacctcac acccgttttc aacccacccc
    15241 agtatggact tgaacacact ttattagggg ttttgttctt caaatcacag caggcagaaa
    15301 atattgttgt ttttcatgtt tatttgacct atactgatag taccaaaagg tacaataaat
    15361 tcgttttcca ccctgtcatc cttacagttt caaggaggta aaaattgggc aggaaaaggt
    15421 caacagcaaa aaactcgcca aagaccacaa aagaatgggc gcaaatcatc gtggcattga
    15481 tcgctgctgt tgcaaccgtc agcgccgccg tgatcgccat cattccggta ttcatcaaag
    15541 atgcaccaga aacagccaca caatctgccg atgcacctga tgcaagttca gcgaacgtac
    15601 aggatgggtt ggtcggtaaa atgccccctg aaaacgcaga tcgctttttg gtgaatcggt
    15661 tttacaagcc aactcaacac atgggagatc gcggggatat tgatatcagc ggcattggag
    15721 ataccgccaa tccaattatc ttcacctact ccaccaatgg aaatggtccg catgaatggg
    15781 actataaata tataaacaag gaaataaacc ccaaccccgc cagatttgca gggatcatgt
    15841 tcattgactc ggctggccca ggtggaacag atcctaataa cggctttgac ttgagcgctt
    15901 ataaaaccat acgctgggaa gccagaagtg ttcaaggcct tgtggatgtt aaatttgtca
    15961 ttggaggggt cacatggaca tgggatgagc aaacaaagga gcgggttgta gctccctatc
    16021 cagattcggt gagatcatcc gtgttattgt gcgaaccaac ccttacccag gaatggcaaa
    16081 gtttcgaata tgacatatca ggatttcccc ccgattcatt tcaacatgtt gtaggggtct
    16141 tcggctggac aattgattgg ggcgacaaca acatcacctc gatcaccaac cctgattcaa
    16201 gcattgtccc tgaaaatccc aaaacccttg tcattgaaat acgaaatatt tattacgaca
    16261 aggcgatcgt agaacaccca gcaaccagcg attgcgggag caaataaatg tccactaaat
    16321 atgtactgca cacacttacc atcatctggt ttgtcctgct gatatcaggt tgtgccaacc
    16381 cacccactgc cacacaaacc agtccatcca cccccacact cccaagcgga tgggaaaaaa
    16441 aagtaagcaa tatcatctgg atcgcctata gcccgcccaa cgcaaacccc aacaagggga
    16501 ttcaggcaag tgcggaggat attcaggccg accttgaggt tttgcacaat gcaggtttta
    16561 caggattagt cacatacgat tcaaaggggg tgatggggcg tgacttcccc actctggcaa
    16621 aggaaactgg tttcaaaggg gtcattatgg gtatttggga ccccaccagc gcggaagaga
    16681 tgtcaaatgc acaggctgct tcgagcaatc ccattgtgct tgggttttgt gtgggcaatg
    16741 aaggcctcga cgtgagatat gacatgactg ttctgtcttc aaccattgaa gaacttcgca
    16801 agacaacagg caaacccgtc acaacagccg aacagcttga tgactattac gatgaaaaac
    16861 tgttggaaat aggtgaatgg gtctttccaa atgcgcatcc atattttcac agccagattg
    16921 accctgtcaa cgctgtcaag tggaccgagg gcgcctacaa agatctgagc agcaaaacag
    16981 accgttttat tctcttcaag gaagtgggac tgcccaccga aggagatgct gaaaataacc
    17041 tgtcagaaac agcacaagat caatactatc aaggcctgga atatacagat gtgaaattcg
    17101 tgtacttcga agcatttgat caaccctgga agacaaatct atctgtggag ccacattggg
    17161 gaatcttttt ctcagatcga accccaaaac aaacagcaat ccgcttcatg ggtggagaac
    17221 ccgtcgtgga attcccaact gcgacagcta cagaaattcc tgtagaaaca gaactcccca
    17281 tctctgatga tattttctac atttacaacg acggagcatc tccttataac catttcagcc
    17341 caagcggtta tatgggcgat actggtgaca ttcaaattga tggagccagc acggaaaatc
    17401 cgcaatcggg cgatacttcc attcaggtga tatacactcc taaaggtcag gggccatatg
    17461 cctgtgcata caaaccacct tgcaaatggg cgggagtgta ctggcagcaa ccagccaata
    17521 attggggcaa ggagcagatc tgggctggaa caggtttcga tctctccgca tatcgaactt
    17581 tgaaattctg ggcaaggtct gaatctacct gcacattgga attcaaagtt ggcggcatca
    17641 ctggagatta tggcgatagt cttgtgtatc cacgcggtat caccgaaagg ttgacaaccg
    17701 aatggcagga atatgaaatt ccgcttgagg gcgcagactt atcctacatc attggcggct
    17761 ttgtatggtc taccagttgg gagaaatgcc cagatggagc aacattttat ctggatgaaa
    17821 tccgcttcga gaagtaggtc actattacat attggctcat tacggcaaag agttcccatg
    17881 cctcgggtat actcccgtac atagggaaat ccatgaatcg cattgaccgt ctctttgcca
    17941 tcttgctcga actccaacat aaacgccgcc tgcgagcagt agatttggcg cgtcagttcg
    18001 agatcagcaa gcgcacgatc tatcgcgata tgtctgcatt gaatcagatg ggcattccga
    18061 tcgcggcctt gccaggtgag ggatttgaac tggtggaagg attttatctg ccgccgctga
    18121 tgttcaacga aaacgaagcc gttgcgctga ctctcggtct gcgcctcttg actcaacagg
    18181 cggctggttc gctcacccaa gccgcagact ctgcctcagc caagatcaag gtcgcgttac
    18241 ccgagcaggt tcgggttaag tcggaggcgt tgagcggcat catcgggttc attacgccga
    18301 gtcagaaatt caatctcgac gactcgcagt tgctcctcat ccaaaaagcg attcaggaaa
    18361 agcaggtgat gcatattcgt tatcacggct accgcaaaga tgaaatcagt gagcgtgatg
    18421 tggaaccgca tcaactgtat tactcagatg gtgtttggta ttttgaagcc tattgccgtc
    18481 tgcggaaaga tgttcgtgaa tttcgtcttt cgcgtgtgga gaagttgacc ctgctcgatc
    18541 agcatttttc aaaacggcgc aagagcaaag cggcggcagc gcggatggtg gaagtgcgca
    18601 tccgcttcga tgcgcggatt ctgcgttggg tgcgcgagcg ccaacattat ggatttgtgc
    18661 gtgaaaagga ttcagtgatg acctatcacg tccatgatgt ttcggagatc acgtcgtgga
    18721 ttcttggctg gggcgcggag gctgaagtgc ttgcgccgaa agacctgcga aaggaacttc
    18781 gcgagacggc aaaaaaactg gcaaacttgt tgacataggg ttgtcacacc tcttttgtaa
    18841 aatgcgggga aattcaatca aggagattta tatgtcaaat caagtaattg aactcgcatc
    18901 atttcggctg gcagacggcg catcggaagc tacattgatc gccgcatcag atgccatgca
    18961 agaagggttt ctaaaatcac aaaagggatt tatcaaacgt gaccttgtca aaggcgcgga
    19021 tggtcaatgg gcggatgtgg tgtattggga cagccaggaa agtgtggacg ccgccatgca
    19081 ggaagccatg aacaaccctg cctgctttaa atattttgag ttgatgacag gtgtgaacca
    19141 tgaagatgtc tctgcaggtg tgatgcattt gagtgtgcta aagtcgtact tatagaggat
    19201 ggaatggtaa ttaaagccgt tttgatagga agcgtattgg gggtattgga atgggtcatc
    19261 cttgtcggct ccaggcaaaa attggggttt tggatatata tccaaagcat gttagcctgg
    19321 ttcacctgtg gatttatgat cggagttgtc gattccccct tccctgctta tttgcttggg
    19381 tctgtgcttg ccgttttctt gaatttaccg tggtatatca acatcagcat cattccaaag
    19441 caatataacc atttgccccc attaattatt tcgagcatcg ttttgggtgc cattggcggc
    19501 gggttgaaaa ttctgttatc tgcgatgttc tagcctacac catttgaaag tttactgggg
    19561 aagcgatttt gagttgaaat aaaaaatctg aggcgttctg cctcagattt tcgttttacc
    19621 agtcattggt agtttcgtac cccagccgca ccaacagatt ccctgtcact tccttgaata
    19681 gttttttgtg ttcttcagtg aagtgttgtt tccagcctcc tgtcttccca gagcggaagg
    19741 tgtgactctt ttttggctga atcgcttcca ccaaaacaga tagcgctttt tcgcgtggag
    19801 tggaaatctt gtagcccgtc ttttccactt catcaagcat cttcaacaag gttgcatcgc
    19861 ggttgttgat gaggtcttca aagcggatgc acatcacgtt tttctgttca agccattgga
    19921 agacgccttc gtagcgttgt ttgacgctga ccatcttcag cccgtcacgg tcaatgcctg
    19981 tgatggcaac attcaaacgc gcggcaaaat caggcaggga cttgtagtat tcgtgcatgc
    20041 cgtgttcttc gtgcatatcg gtcgcgaaga acacttgtga gacgagcata tctctcgggt
    20101 cgcgataaat aaaatagttc acgcgcccag ccgcagttaa gaaagaagcg ttttcttccg
    20161 tcgcatccac atagccccag ccgatcactc ctttgggagt gtgcttcaag tctgcaagga
    20221 tttcttcttt attctttcgt cttccatcat atgtgatcgt ccgcacgggg tcggcatcca
    20281 catatttgta cggcatgatc tgggtgaacc cattcatcac ttgcaaaagc aaatgcgaac
    20341 cgctcttcgg ttttgagttg gcaaagatgg gcggggcatc gttaaaagaa aaccgcttcc
    20401 aacgccagat ggcttgggcg gttttgccgt aagggcggaa ggcgcggcgg atgttctgtt
    20461 taatacccat ggatactttg aaccgcgaag cacgcgaagg gcgctcaaaa tttcttttct
    20521 ttgcgctctt ggcggtcttc gcggttaatg attttttatt cgctcaccac gcggcggagc
    20581 ttcttcatgg agcctttttc agattcgtaa accttcttca cgccatcgcc cattgccgct
    20641 tcggtaacgc ggatgtattt caccaggcgt tcgaaaccac caggctcaac agacgcagct
    20701 tgatcactgc cccacatggc gcggtcaagt gtgatgtgac gttcgatgga tgtggcgccg
    20761 agcgcaatcg caactgcgga aggcacaagc cccacttcat ggcctgaata accgatgggg
    20821 ttgtttggaa attccctgcg cagggttcca atcatgttga ggttcaattc ctcaggctcg
    20881 catggatacg tgctggtgca atgcatgatg acaaggttgt cttcaccaac cacttccacg
    20941 ccgcgtttga tctggtccat ggtggacatg cccgtggaga tgatgacagg cttgcctgtc
    21001 ttgcgaacat atttcaagag gttgtgatcg gtcagggaag cggaagggac cttgtaggcg
    21061 ggcgtatcga acttctccat gaaatccaca gagggttcat cccaaaccga aaccatccaa
    21121 tcgatcttct tttctttgca atagcggtcg atctcgcggt attgttcttc gctgaactca
    21181 accttgtagc gataatcgag atacgtgatg taaccccatg gcgtctcgcg catttgtttc
    21241 tgttgttcgg gcggggtgca tacttcgggt gtgcgttttt gaaacttcac cgcgtctgcg
    21301 cctgcatgag cggcggcatc aatgatcttc tttgcaatat ccaaatcgcc attgtggtta
    21361 atgccaactt ccgcgatcac gtacgcaggg tgaccatcac ccatcattcg atttccaaat
    21421 ttaatttcac gagccattcg tattctcctt ttgagataat tgtttcaata caatttcgca
    21481 caactcacgg atcgctccgt ggccgcctgt tttcgtcaac acatgatcgg cggcacggat
    21541 gacttcggga tatgcatccg ccaccgctac cgaccatcca gcgatctcaa aacagggaag
    21601 gtcgttgagg tcattgccga catagacgac gttctgcgct ttgacattct tctgcgcgag
    21661 gacttcgcgc atcactctac ctttgtcctg catgcccaca ttgtggatcg cctccacccc
    21721 catctttttc gcacgcgcca acaccaccgg gttcggctcc gaagagagga tcatcacctc
    21781 gacacccatc tcgcgcagtt ttttgatatg catgctgtca ctgcgcgagg ctgtgaccga
    21841 ttccctgcca tcctgatcgg tgatgacgag attatccgtc actacgccgt cgaaatcgca
    21901 gatgatcatt ttcacggttt caggcatcag gcggcggggg gcacccggag agaccatttc
    21961 caaaccgctg tacacaagcg cttcatactt ggcccaatcg ggcaatgtat caatatccac
    22021 tgtgtatttc gggtcgatca ccaacggata gatgacatca cccgtcagag aggccttttg
    22081 cgcgatcgtt gccgtacgga tcgcatcaat gtgcccggtc tgccagtaaa cgggcggcag
    22141 aatctggcgc ggagcattat atggttcggc aattccatcg acggtcaaaa gcggattcag
    22201 aggtttgcct tcaccgtgaa aacgccacat cttgaaaggg ttctgcccgg caggcaccac
    22261 accgcgcaca caatcggcat cgggatgctc cagcaaaatg cgaatggcat cgtccaccat
    22321 cttgtgcgga cggacgggag aggtggggcg aagttggatc acaatatcgg gacgatagcc
    22381 ttccacatct tcgagccact tgagagcatg ctcaaatacg ggcagatcgg tggtcttgtc
    22441 ctgtgcaaat tcggcggggc gcatgaaggg agtctctgcc ccccactcac gggcaaccgc
    22501 agcgatctcc tcatcatctg tcgagacgat gacacgcgtc accaactcag attgttttgc
    22561 cgcggcaatg ctccacgcaa taagcggata ccccgcaaaa ctgcggatat tcttgcgcgg
    22621 aattcctttc gaaccaccgc gggcggggat aagagcgaga atgtctgtca tagttttctt
    22681 gcagcgacac agcggcgccg tgtcactacc tttgttttac cacgccgtcc agccgccatc
    22741 tacaatgaca ttattgcccg tcatgtagga agaagcatca gatgccagga acaataatgc
    22801 accgttcatt tcatccttct ttgccatgcg gccaaggatg gtcttggcgg aataattctt
    22861 cacaaaatat tcttcatggt tgttaaacac accgcccgga gtgagcgcat tcacgcggat
    22921 ctcggtttcg gcataatagg cggcaaggta tttggtaaat cccatcacac ctgccttggt
    22981 ggtggtgtag taaacaggtt tgtacgccac acgtttgccg tctttgatgt aaatccgctg
    23041 atcggggccg ttgagcccat acgtagagca gacgttgatg atgctgcctt ttttgccctg
    23101 cgcattcatt tgcttgacac aagcctgcgt tacgaggaac atccccgtga ggttgacatt
    23161 cattgccgcg ttccattgct caagcggata atcctcgaag gcaccagggg caattccctt
    23221 ggcggcggca tcggggtcga attttggatc aagggcggct gaattcacca gtacatcgag
    23281 cctgccaaag gtcgaaagaa ccgaggcgac catcgcgttg accgattctg gtttggtgat
    23341 atcggtcgcg attcccatcg ccttgtatcc agatcggacg agtccatctg ccactttgct
    23401 ggctgcctca ccgttcaggt caacaacagc aactgccgcg cctgcctcgg caagggtctt
    23461 gcaaaattcc gcgccgagca gccccacccc gcctgtcaca accgctacgc ggtctttcaa
    23521 actgaattta tcaaaaatgg tcatgtgatt tccttatgta gcgcgacata gtatgtcgcg
    23581 ttacggctga tttgtataaa tcttatcgcc gcgtttatcg agagccaggt tcaaaccctc
    23641 ggccaattgc agatagatgc cttgtacatc ctcttcttcc gcgccgaact tgaacaaagc
    23701 gaggacggca ttccctgaaa gtacatcctc ctgcaaggcc tgtacacctt cttcgtaacg
    23761 ttcgcgcggc agccctgtca cagggtcaac cagatcgggc aaaacatagg cgggacgatt
    23821 cgtgtaaagg taaacggggc cagcctgatt ggtgtaaata cgaatgcctt caggaagcgc
    23881 actcagaaaa tccatcgcag ccgaatcata ccattggaag gatgcatatc cctgcccgcc
    23941 tttgtgaagc tgacgcacga ctcccaccaa ctcgcggctg gatagcccca caagaaacac
    24001 agccgccccg acgacgatcc cgcgccaaag cagggtccgt ttttgccaga gccagaaccc
    24061 gaaccacaca agcaggatca acagcgaaat gtagatcggc gcattgatcc gcgctttgaa
    24121 tttggtactg gcatcgaaca gcatcatcga agaaagaatg gacgccagat aggcaaaaac
    24181 ataaaggcca ttcgtgaagg acaaaatttc gggcattggt gaagagggtt tgaaaaattt
    24241 cttcaacccg gtcacagcca cccaaacgag aatgatcagc ccgagaatgg caagaatgac
    24301 gaacagcagg tccaggtttt ttaccaaagc ccgccgccag gcctcgatcg ggacgagaaa
    24361 ctcggaaaag ttgtaaatcc ctgcctgaat attctcctca gtcagcgggt gatagacgag
    24421 cgtacggttt gtggcattat ctgccaccaa tttattacga aaactccacg ccgccgccca
    24481 cgggaaaaac gaggcgatga agatcagcga acgggtgatt cgcttcaacc agttttcacg
    24541 cagcaaaatc agcgccgcga gaaaagtggc gaacaaagcc agccctgcgt aacgcgtcag
    24601 gtaggccgcc ccgacaaaac agcccgccag aatcagccag cgtttttcac gctgctcgat
    24661 ataaaattcg aacaaaagga atgcgccaag gctgaaaaag atatacagcg gctcgctcat
    24721 cgccacggca tggatgcgca acagcgaagc gttcaacaca aaaagcaggg ccagcacaaa
    24781 ccccgccgcc tgcgacttgg tcatgcgcca gccgatcagg ccgagcaaaa aagtatttgc
    24841 gccgaacaaa gacgcgttaa gaaagcgcgc gccgcgtacg gggtcaagtc cgctgaggcc
    24901 gatcaatgca agaatggatg aaaaggctgg gggaaagtgt gtgaccgggc cgttactcgc
    24961 aagccatgct tcgcgatagc cctgtccgct taatagactg cgtgcgcctg cgatgtaagc
    25021 gatcgaatca tctgaaaggc cgaggccttc gggagtcgca taaaggatga aaactgttcc
    25081 cagtatggcg agcaggccga gggtggcgaa agatgcggca cgttgtcggg tcataaaaga
    25141 taaaaagacc tgacaggttt ccacagagat ggaatcgctt ccatgtctct gatccgccgc
    25201 accgcaattc agcggcatgg cgcaaaacct gtcaggtctt gggtttactt cacaggcaga
    25261 atgtagccct gttcttcgag gtacttgacg atgaggtggg cattctcttc aggagaagcg
    25321 ccgaaaccct tgagggtgag ttcgggatcg atcggcggtt catagggatc atccacaccg
    25381 gtaaagccca tcggcttgcc atcctgcatg gcctggcggg ccttggcata caaacccttc
    25441 acatcgcgct gttcgcagac ttcaacaggg gtatccatga agacttcgat gaagttctca
    25501 ccgaccatct tgcgggcttc ctgacgggtg gcgcggtagg ggctgatcgc cgcgcagatg
    25561 acgatgccgc ctgcatgggc aatttcgccg gccacaaagc caatgcgaag aatgttggta
    25621 tcgcggtctt ccttgctgaa accaagaccc ttggaaaggt gcgtacgaac cacatcccca
    25681 tcgaggatgg cggtctcgcg gccacgttcg aggagcaggg tggtaaggac ggcagtggtg
    25741 gccgatttgc cagagccgct caaaccagtg aaccagatcg caaatccctg cttgtggcgg
    25801 ggcggataca tctcgcgcaa aatttcggcg gtctccgggc gggtgaacca ctcgggcaga
    25861 agtttgccct tggcaagata atcatcgcgc acctgcgtgc cggagatgtt ggcggtcttt
    25921 gcgccctttg gcacgtcctt ctcttcgacg taacgatctt catcgggaag atagacgagc
    25981 atctcgaacg gaaccatctt gactccgagt tcggcttcgt acttcttcat attctcctgc
    26041 gcgtcatacg gaccgtagaa cggtttgccc gacgaatcat ttcccggccc cgcatgatcg
    26101 cgcccgacga cgaagtgatt cgcgccgtgg ttgcggcgga tgatggcatg gagcagggct
    26161 tccttcggcc cggccatgcg catggccagc ggaagcaggc tgagcatggt gctgttcttg
    26221 tcgtagtgat tatcgaccag cgccttgtat gtgcggacgc gggtgtagtg atccacatcg
    26281 ccgggcttgg tcatgccgac ggtgggatgc accaacagcg agccgttgac ctgcgccgcg
    26341 gcgcgtttgg tcaactcttc gtggatgcga tgcaggggat tgcgcgtctg gaacgcgacg
    26401 acgttgtcat gtcccatttc ctccagcctt gcgcggactt gcgcgggggt gtggcgcagg
    26461 ttgacgaagt cgtaatactt gggaaggttc acaaccttca tcgggcctga gatgcagacc
    26521 ttgccccaac gtcccatttc agagaccatc gggtgcttgg aatcggtgga gccgtaggca
    26581 tgtgcagcct cggtatcggc atcccagtgg aagacctctt caagggtcat caccgcgatc
    26641 aggtcgaagt tggcattgcg cagggctaat tcttcgccga cggtcggcaa ttccttggga
    26701 tcagccgtca gggtgatggg aagcgggaag agggttccat ccgcaaggcg catttccttg
    26761 agcacacggt cataatcggc cttgcccatg aaagtggtga gcggtgaaaa accacccgtg
    26821 gcgatcaatt ccaggtcgca caggttgcgc atggtgatct tgatggaagg aagtttaccg
    26881 gcgcgggaga gaaggtcctc gcgttccttg ccttccacca caaggttgac cagttttcca
    26941 ccataaggag gaataagttt tgcttttgcc atttttgctc cgttcgttta ataaaaaatg
    27001 tttttttatg cccacccaca ccttgaggta ttgagaggct tttgattttt tggttttaca
    27061 agcgcccatt atactccaaa attcagtcaa tgaattttaa acggcaaaga gggagcggct
    27121 aaacctgtgg ggtaaaaaca caaaggcgcc aagtcactaa gccactaaga atcctttgtg
    27181 tcttcgaggc tttgtgactt tgtggcaatc aaaacccacc cttttagcca catgaattgc
    27241 tcccttcacc gaggacggcg gcttgagccg aattttaaac ggctaagata taatcccaaa
    27301 accatgaatt cgaaattcct tcgcaatgcc ctgtttgccc tgcttgtttt tggctacatt
    27361 tactactgcc agcgttatat tgcagccacc agtttcatca ttgaaggcaa aacatattac
    27421 gtgcttttcg acgacgccat gatctcaatg cgctatgcct acaacctcgc gcacgggctg
    27481 gggcctgtct ggaatgcagg cgaacgtgtg gaaggattca ccaacccgct ctgggtcgga
    27541 ctcatggctc tcgtccacct gctgccgatc ggattgaacg aaaccagcct tgtgatgcag
    27601 atcatcggcg cttcgctcct gaccttgaac ctgtttctcg ttaaaagcat tctcgaacat
    27661 tttacagatg acctcttcgc catgctcgcc gccgtggcat tgaccgcttt ctacgcgccg
    27721 ctcaactcgt acggcctgct cggcatggaa gtgagtctgc tcgcgctgat gttatccacg
    27781 gtcatctggc tgatcatacg gaacgcagac ttcagtctgc atcccacccc ctgggtgtat
    27841 ggaattctag ccatttccac ccttgtccgc tttgatatg
//
LOCUS       DAOQJC010000050        27717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_755_length_27717_cov_10.006471, whole genome shotgun sequence.
ACCESSION   DAOQJC010000050 DAOQJC010000000
VERSION     DAOQJC010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 27717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27717
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_755_length_27717_cov_10.006471"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /locus_tag="PLE39_09430"
     CDS             complement(<1..1492)
                     /locus_tag="PLE39_09430"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNE68591.1"
                     /translation="MSKFDRRSFLKLIGAAAPAMLLPSLAHRIGGLDASRPNIIVLLF
                     DAMSARNLSLYGYPRPTVPAMERFAEHANVYHAHNSSGNYTIPGVASLMTGTYPWKHR
                     AINHRGAIRQSMVENNLFRVLGSDYHRLAFPQNFWATLLLSQFQGDIDVLLSSGEFSE
                     LSYLLSDHFPKDKNMSARALDDFLFRVGEAPPSLVLGSLERLLYTWQSNHLPTDGYKE
                     LPHDLNYPITFRLEDVFDGLISLIPSLPAPFFTYLHLLPPHTPYRPTDKFFNAFRDDM
                     KPMRKPDHRFADGTSNADLNLARRLYDEYIASLDQELGRLLDSLESQGLFENSYVILT
                     ADHGEMFERGEKGHSTPLLYDPVIHIPLLISAPGQKTRRDIYTPTNAVDILPTLAHLT
                     GNPLPAWTDGKPLPGLGGEEDYERSLYSVEAKLSPANGILNKATVALRKGDRKLIYYT
                     GYEAEDSFELYDLSENIEELHDLYPDQPAFAKQMKEELLEAFFEANK"
     gene            complement(1536..2069)
                     /locus_tag="PLE39_09435"
     CDS             complement(1536..2069)
                     /locus_tag="PLE39_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68592.1"
                     /translation="MDIVKKIPRWEQVIPVYAVIVMMVYVWSLFQYFWRLSSWLNFST
                     LGQVGGIYVYTVAVNFVESLLVLLVLLALSAVLPATWFSDEFVVKGSTLTLLVLGGLM
                     VFNQTLFEDVLHPSILLPKVLAFAAVTAFLVFLFGRVGFLKKTMEEVANRMTVFLYLW
                     MPITAISILVLLTRNIF"
     gene            complement(2170..3198)
                     /locus_tag="PLE39_09440"
     CDS             complement(2170..3198)
                     /locus_tag="PLE39_09440"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNE68593.1"
                     /translation="MLKRFKLAAKILLRGEPQKKNRNPIPAITAEEVAEARAFFPRDK
                     FFIFGHARSGTTLLMRLARLHPEIHCNYQAHFFTRKPLLKSLVDSPEAEEWLTRKSNR
                     WNQGRDLSPLVLRAAADFIMERDAAREGKMIVGDKSPSSTIHGQAVRDMHAVYPDAKM
                     VCIVRDGRDVLISERFRNFVEESRFLTSEDKRIISDLRLDPVPFSDGRRSIFTETFIR
                     RVAKDWVNDLTETEGEGRRLFNDRYFGMRYEDLLAQPFDEMSRLWKFLSVQSVDPSLD
                     SSLRAEMSTNPDEEWQSKRNEGIASFLPKGQAGNWARLFTERDKAVFKEVAGEMLMHW
                     KYEKDLNW"
     gene            3578..4525
                     /locus_tag="PLE39_09445"
     CDS             3578..4525
                     /locus_tag="PLE39_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68594.1"
                     /translation="MPPSVTTQVAEVAPLEVCTPVPSENVNVYRTIPQPWAGLIFDYA
                     HATLPATSPTLDPLLAAAFPAITQLQGAQYAAFQKLVSETKRWSDTETIKLDDAENKV
                     RITLTFISPQLLQAVYLNQVLKDGYPASGFLEQLQSMLNNVAARDELLFLLTISPLSS
                     INPIHHTVELPIDSIVLNNSENLQVSHSHDDHNLAQPFDTSSDAIFGYLAFPFTQLVN
                     SECKWILNPKFNTNIVVTMPYLKVDGNKNGISYSWTIPYTSLINQTVTLDALNFNPPP
                     GYDFTQVTPSSLPPTDISSPSSYDESFAKFIWKQITGSY"
     gene            4529..5539
                     /locus_tag="PLE39_09450"
     CDS             4529..5539
                     /locus_tag="PLE39_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNE68595.1"
                     /translation="MKTIFTFVPSKTHEFPDHPERPGRLDVLEPLLASVDAERIKAVP
                     AAPEQIALAHKPQLVKAIAEVCTRGPGIIDHAPTFITQTSYEDALLAAGGVVTCTRAV
                     LNGEAQNAFAIVRPPGHHAEPDRAMGFCIFNNIAIAARDALANGLQRVMVIDYDAHHG
                     NGTQAVCVDDDRFGFISTHQWGIYPGTGWINDAPHAKGRLVNVPLHEFAGDGTFARVA
                     DEIFKPIVESFRPQMLLISAGFDAHWNDPITSLGLSTRGYYEISKKLVALANEHCGGK
                     IVFVLEGGYDPHNVANGAMAVFDALTNSPLNKDAGDPSPYPDSDHESRIHDILKWHGI
                     AQ"
     gene            5583..6659
                     /locus_tag="PLE39_09455"
     CDS             5583..6659
                     /locus_tag="PLE39_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE68596.1"
                     /translation="MQRKQSKGFVELEWECPNCHSANKGSKKICESCGAPQPEDVKFQ
                     RAADEKIITDEKVVSAASAGADVHCGFCGTRNPATATNCSQCGADLKEGKARQAGQVL
                     QAAPESAKIITCKSCGTENPGGERLCNKCGAPLPRAESVAAKPAAAVAANSGTSSAAA
                     PKAQPKKTNWLLFGAIGAFLMVCCIAIFAMFVIPSKSVQGTVADVYWQTSVPLQEVRA
                     VDYSNERGNPPAGAYNVSCHTESQEICEDKTVDRGNGFAEVITECHTESQDYCSYTVD
                     EWTTIQSYDLSGSDLFPEYAQPSVFNGQRLGNASATLTVTFDTPKGTITYTPGTVSEF
                     QQFQIGSTWTLKLNALGGVLSVGK"
     gene            6670..7173
                     /locus_tag="PLE39_09460"
     CDS             6670..7173
                     /locus_tag="PLE39_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68597.1"
                     /translation="MTDRAEDSLKNDVLYTDDLALNRNGQLSETQKKRLWPGALFWLM
                     LAGINVIFLLLLSFFQLRYQTVGCGYLGLFVIAIFSIRQCILNSVPYREDIKNGIVKT
                     ASGQAFKRAVLQSFSKGVYLGNCSVRVNNKVFTISPHFYNWMVDEGYYRIYFTPKTKR
                     VVNIEEL"
     gene            7250..8476
                     /locus_tag="PLE39_09465"
     CDS             7250..8476
                     /locus_tag="PLE39_09465"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HNE68598.1"
                     /translation="MNHSVAIIGGGPAGLMAAEVLSGHGMQVDVYDSMPSLGRKLLMA
                     GKSGLNLTHSEPFEKFVLRYGSKKQEVETWLNDFTPDDLREWARGLGVETFVGTSGRV
                     FPKEMKASPLLRAWLKRLTAAGINFHLRHRWNGAIAAGEGGGSIVKFETPDGIKTVHA
                     NAVILALGGGSWARLGSDGAWVEWLDQAGVKVEALKPSNCGFDVAWSLHFREKFDGQP
                     LKSVTVSFGDFHQQGEFIVTREGVEGSLIYAASALLRDEIAQNGRAILELDMSPDRPL
                     EWLVERLSKPRGSRSMSSHLEKTVGLKGVKAGLLYEFISKDDFANTERLAFFIKHLPI
                     PLIATHPLDEAISSAGGVSFKAVDENLMLHKLPGVFCAGEMLDWEAPTGGYLLTACFA
                     SGRVAGLGALKWSNAG"
     gene            8498..9139
                     /locus_tag="PLE39_09470"
     CDS             8498..9139
                     /locus_tag="PLE39_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNE68599.1"
                     /translation="MKNLDYRDYYRRNLPHFQPRGATFIVNFRLAGSLPVEVVKMLKA
                     ESEEIEKSLLRIEDHTERYHVKDREQLRLFSKWDEALHNSQAGPFWLKDERIAQIVVN
                     SILYHDENWFDVLAYCIMPNHVHLILAPRENADADFSLAKIMHNIKRNSANHANKILE
                     RTGAFWQHESYDRFARNEAELERMIKYVLYNPVKAGLVDDWKKWKWSYCKFEM"
     gene            9181..9612
                     /locus_tag="PLE39_09475"
     CDS             9181..9612
                     /locus_tag="PLE39_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNE68600.1"
                     /translation="MHEDILKIQQLIARFANSFDVKDWDGLQACFTESLYTDYSDLRG
                     TPPETISASEYVRLRRESLDHLKLHHLVSNYEITFPGFDVAACRASMVVWRRSEAEDF
                     TTHCIYDFQLARQRGTWKISGITQKILWNDGNASIHKGAKN"
     gene            9615..10550
                     /locus_tag="PLE39_09480"
     CDS             9615..10550
                     /locus_tag="PLE39_09480"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE68601.1"
                     /translation="MYRKGWRYYRDATLLWILFFVVLMIGGYALEHPLYWILALFVWV
                     VRSAFGNAFQFAHPSRRFNWDSRSNLVFKEVTFKSRDGLTLFGRFHAGRTRATIILIH
                     GLGGAGTDMLVHASFLVDAGYGVFMIDLRAHGSSDGDTSTYGLREADDVAGAVDYLLS
                     RMDVNGAKIGVLGISLGAQAALRGALKTENIRALVLDGLSPARLSDHGGSPKSIQRWL
                     NYPFNWLYYRIYEYMIGGRDAGVLEVIGQIAPRPILLIASGLRDIYFSRLFYEAAKDP
                     RALWEIPDGIHGVALAGNPQEYIDRVVGFFNQTLL"
     gene            10547..10972
                     /locus_tag="PLE39_09485"
     CDS             10547..10972
                     /locus_tag="PLE39_09485"
                     /inference="COORDINATES: protein motif:HMM:NF039927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HNE68602.1"
                     /translation="MTACPECGATNCESRFHEFLALEFSNAAYGNVHHLTVAAYMLQH
                     SSKLTREGWRYERELLRAFLVENKTPAFIRRQNRDWVDGGKRTFKIKSKDGSPFINRF
                     AWTKTILNVRAENAEVYCADITTWAGAVLKDAEEIKLEI"
     gene            11116..12507
                     /locus_tag="PLE39_09490"
     CDS             11116..12507
                     /locus_tag="PLE39_09490"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HNE68603.1"
                     /translation="MKRALQTSFIIWTILSALVLASVIVIYLRNGETGLNLFIQQWPL
                     SNLTVTLEATAYTWLCAGAILYLLANDKVNAENAFFVAGFFIVILLYLNVLRERFRYG
                     DYQYYLEAATALMNNQPLPDTYLYLPLWATLLQFIVPLGDQGMLAILWTVNIIMLGSF
                     YILLVRVLEHYGYSNRFAVIIAVLFMSINTPLHRSLGYVQVNLIAMVLIMASLLTFRK
                     NDLVSALLLALAVHLKTSPAALALAFLLERNWKWLAWFALTFILIALIPVALEGTRPY
                     FDYLTNINILTHITDTNFHDTSFDSFFRFFNPFLGINLEATRLITMGAKALLGLAAIY
                     VMAQNIRSHTFVSRETQNHILLNAIPSLFIVMTLTSPIVWDHHGIFTTLAFLLLLHCI
                     ETPALWMWFGFAYFLEFILPSFDFFPWSFGRLIAPLIVLCLMWYVTRSKQSPSLLVSA
                     NRWFENLKFQISQ"
     gene            12504..14249
                     /locus_tag="PLE39_09495"
     CDS             12504..14249
                     /locus_tag="PLE39_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68604.1"
                     /translation="MKQQRPILILLAVSLLIGIFTLNGYGDSWDDLSLRKYAAKSLEA
                     YGTWVARGEVQITTEDLGSYGPSYVMAVALLSQDNPDLRHFIYFVTFLAGAWAFYMLG
                     LRWLSQPAALGATLLYLTQPVLWGHAFFNPKDTPFLSLFLLSIYFGLKLFDESPNPIQ
                     GSDRRTALLTALWLGSVFSLFLLTDTFHTAIVNLVHAAKAGETNIISLTASDIRTAAP
                     ELYIQKYFTLFLRLRAVFFLLSSFFFLFHKSFRSLHPSAFTRSVYPSVFLGFTSSLRV
                     LGPFAGLIVAYYALRKHGRKSIPTLIAYAVTAIIIMYLTWPYLWTNPLGNFIASIKTM
                     SLYPWSGQVLFNGQQFASTELPASYLPVLLGLQLTEPLWALTLIGVVVAGIRLREKRE
                     LIELSILWLILPLFGFIVMRTALYDNFRQVFFILPPLFWLAGVAIEQVKRPAFQMALI
                     VLAALPGIVDGIRLHPYEYIYYNRFIGGVDGAQGRFELDYWGASYREAAEFVNTTAPA
                     NATVWIEGPSQLFELYAREDLKLYSTHEADRADHYDFAVALTRDHMDQASFPNAEVIY
                     RIMRGRAVLAVVKTP"
     assembly_gap    14317..14416
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14417..16287
                     /locus_tag="PLE39_09500"
     CDS             <14417..16287
                     /locus_tag="PLE39_09500"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HNE68605.1"
                     /translation="LKRENIMNFLELLSQKTVVADGAMGTMLHAHGVDFGKCFDELNL
                     TNPAAVADIHREYIEAGAELIITNTFGANRYKLKKNGLADKVKEINHAGVELAKRIVA
                     ASFKEVLIAGDVGPLGVRIAPYGRVKPEEAREAFAEQIRALAQAGVDLIVIETMSDLY
                     EIQEAIKAAKQACTLPVVASVTFTRDDRTLLGDDPMKVARALHGAGADVIGVNCSGGP
                     SQLLRILKQMRQAVTDRSAKFWVKPNAGWPEQVGGRIMYPADADYFGDYALAFREAGA
                     NIVGGCCGTTPQHIAAMKKAFATTPVQISEMWEASEVLETESQDVEQPTQLAQKLAAG
                     KFSICVEMDPPRGLSTHKLLAGASLLYDAGADVINVADSPMARMRMSAWAVCDLVQRQ
                     IGVETTLHFPTRGRNLLRVQGDLLASHAIGLRNIFVVMGDPTSIGDYPEATDNYDLVP
                     SGLIKLIKQGFNAGVDHSGTSIGQPTNFFVGAALNLCPQDMDTEVKNLHRKIKAGADF
                     FLTQPIYRADDGPKLIEAYEAKHGKLQQPVLAGILPLVSAKHANFLHNEVPGIFIPEE
                     ARKKIEAAGDAERGVKIGVELAVELIEQIKGWANGIYVMPQFHRYDMVAEIIEKIR"
     gene            16336..16761
                     /locus_tag="PLE39_09505"
     CDS             16336..16761
                     /locus_tag="PLE39_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68606.1"
                     /translation="MWKKTLQKIILILLVLLGIKTGVQGLASTINMPSHDVSVAKWDE
                     RAAALKDALPFKRGFVGYISDIDIPNVEYDRQNLVGEFTLTQFSLAPIVLIQGSDDEE
                     WNILSLSQQAYEIWSAENAAQYEVIKFGGKFYLAHKVTP"
     gene            16758..18158
                     /locus_tag="PLE39_09510"
     CDS             16758..18158
                     /locus_tag="PLE39_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68607.1"
                     /translation="MTAALSLLSFALPMLTGILLIHALWAEKNNFFDWVLKLSLGTGI
                     GLGISSLLYFVYLLAFAGKPWFLFVEVLTFGAVIIATRKYLRVSVSLWFKPTRAQTWA
                     LILAGIVFFTTVAGILNYSRQRALGDWDAWMIYNRAARFLFRDQAAWQESFPPEMSVI
                     FHADYPLLLASNNAARWDILNKETPYVPMFQSVLFAFAALGLIFGALGRFTSVGQASL
                     GLILLAGVPFYLLEGGRQTADLPLAFYFLATVVFIFAYYEEKRFSLLFFAGFTAALAA
                     WTKNEGLLFLLVAGAVILLLRTADFSLRIWHKQVFQSFLPFFAGALLPLSVALYFKVA
                     LAPASEFLSGGLTKILQDIADPARHGIILSYFKNIFLFSNGWYRVGILPILCVYFLVF
                     HTRKAQGNNPVFPGFAIFTLQIIGYYAFYLISPYELDWHLSSSIDRLFIHVYPTILFV
                     TLAASQTPETIFESDV"
     gene            18251..19015
                     /locus_tag="PLE39_09515"
     CDS             18251..19015
                     /locus_tag="PLE39_09515"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNE68608.1"
                     /translation="MLLTIDVGNTNLTLGLYEGEQLGAHWRLATDHNRMPDEYGLQFL
                     GLLQNAGKTLKDIHGISLASVVPPLTARVIQACREYLRQEPLIVDAGVKTGIKVRYED
                     PKAVGADRICDAVAVMKLFGGPACVVDFGTATTFNAVTKDGQYLGGAITAGVNLAAEG
                     LFTHAAKLPRIDLQRPPSVIGRNTVHAMQSGLLFGYVSMVEGMVARFRSELGSDMKVI
                     ATGGMAEVIAKETQVVDHIAPWLTLDGLRIIWELNQ"
     gene            19023..20030
                     /locus_tag="PLE39_09520"
     CDS             19023..20030
                     /locus_tag="PLE39_09520"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNE68609.1"
                     /translation="MKKIFALFLGMSILLSGCGTGLFAEPTSTPTMTFTPPPPTEAER
                     ATPTQTFTITPTRFATASPSPTPTWVHQGPESVEVPILLYHWIAVSPDEGTNYKSPYY
                     VKPEVFDAEMKLLHDWGYTTITTEMLIKAIREGADLPPRPVIITFDDGHMNNYTNAFP
                     IMQKYGFTGVLYLVANYLDAEDYLTVDQIKEMAAAGWEIGSHSVSHSDLTSLEPERQR
                     FEVVDSRAMLESKLGVPVLTIAYPFGVSNSSVIDYAHFAGYIGGMGLGYTHDQGLTNL
                     FNLQRRDVKGTYDVKQFASFLPWQGDAIFLPTDTPTPTMTPTRTPIPTYTWIVPSTST
                     P"
     gene            complement(20249..21172)
                     /locus_tag="PLE39_09525"
     CDS             complement(20249..21172)
                     /locus_tag="PLE39_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE68610.1"
                     /translation="MKSSSQLSRGYVIALTATIIWSTTGILISYLSRDYSLPSLVLAF
                     WRDLFVSFGMAVGLLVFSRPLFQLDRKHWGFMVWYGLTLAIFNSMWTFSVQYNGAAVA
                     TVMAFSSPAMTAILSRIVLKEQFSRIKIVSILLSLAGIIFVSGAYDPSAWNLNPLGII
                     FGLLSGLLFAIYNLEGKHASDTHINSWTALLYSFAWATVFLFFFNLGNDLFLTGKGAF
                     ADLLWLGDSVSGWGWLFFLGVAPTLGGFGLYTLSIRYLSPTTSNLIATLEPAFTAIWA
                     YFLLNEVLTGMQLFGGALVLMGVVLLRFGEK"
     gene            complement(21227..21299)
                     /locus_tag="PLE39_09530"
     tRNA            complement(21227..21299)
                     /locus_tag="PLE39_09530"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21263..21265),aa:Glu,seq:ctc)
     gene            21409..22359
                     /locus_tag="PLE39_09535"
     CDS             21409..22359
                     /locus_tag="PLE39_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68611.1"
                     /translation="MTFPSFLKLTPEYRDYVWGGEKLRPGHSPTAEAWVVYENDRIES
                     GALAGKTLSEAANEFGAALLGEKVFARTGTRFPLLVKLLDCAQWLSVQVHPNDEQAVQ
                     LEGPGQFGKTEAWHVLEAQPDSKLIAGIQPGASAAEIAEAIRNGTITEYSQYASVQQG
                     DTVFMPAGTLHALGPGLLIYEVQQTSDWTYRVYDWGRPQTETRRLHIDKSIAVTRHDL
                     AAPIISAPPSGDGTRHILTQCEYFTLELISASTRTVELDTQKESFHALTVIEGRAELV
                     SATETVTLEKFQTALVPASLGSYQLRPLEEEACRVLKSSV"
     gene            22643..23995
                     /locus_tag="PLE39_09540"
     CDS             22643..23995
                     /locus_tag="PLE39_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE68612.1"
                     /translation="MTINDKTVTIIGAGMAGLSAALDLHRAGWQVTVLEARNRVGGRV
                     HTVRSFSNGLTAEGGGEYIDEDHTRMISLAREFNLSLGKVGSWQSQNGDWGAFEGKAG
                     SIADSTLWGTNLASEIHNMWQAVAELGKEVNDPSNPQTARRAEELDHRPASEWIAAQP
                     VHPLARKAFIQHIRSEYTCEPEAFSLLDLARNASFYYSDAEAWPQTHRIIGGNDQLPQ
                     AMAARLPDVRLNAEVVSINLQPQKVTVAYKQGEVYHTINSVFAILAIPLTTARLIDFK
                     GSLPSAHQKMVNEISYGKVTKVMIEYRNRFWKDKKWNGRLTTDLPIVLTWDATSHLEG
                     ERGILTAYTGGGPGAYLTGLSDEERTKTAIAVIEKIFPGSSDLIESTATIAWGNEPFS
                     RCSYMALAPGEVTAHWKTLFSPAGRLHFAGEHATPYQGYMEGAVESGQRAAKHIMESV
                     "
     gene            23992..24693
                     /locus_tag="PLE39_09545"
     CDS             23992..24693
                     /locus_tag="PLE39_09545"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HNE68613.1"
                     /translation="MSESSKAFWEGVRAEFPLLIGVFPFGMIYGALALNAGLSTLASQ
                     MMSSIVFAGSSQFVTAQLVHDAAPGLVIIVTIAVVNLRHMLYSASLAPYLKNLSLQWK
                     ALLSYLLTDEAYAPSVLKYEAEGINASSHWFLFGAGLSLWSTWQVSTAIGIFLGTSIP
                     ESWSLDFALPLTFIAMVVPALKNRPMIAAALSAGGVALLAHNLPFKLGLILAALTGIV
                     IGTLFEGRQDEKGAQ"
     gene            24690..25001
                     /locus_tag="PLE39_09550"
     CDS             24690..25001
                     /locus_tag="PLE39_09550"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HNE68614.1"
                     /translation="MNIWLVMVIGGLITFGMRFSLIYLFGRFEIPETMRRALHYVPPA
                     VLSAIIFPELLYHANQIDFSFGNIRLLAGIVATLVAWFTKNTLLTILAGMLALFLFQL
                     L"
     gene            complement(25080..25985)
                     /locus_tag="PLE39_09555"
     CDS             complement(25080..25985)
                     /locus_tag="PLE39_09555"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE68615.1"
                     /translation="MKILVLENDPNEFAIIQQAFNDGKTSLIPIASSEQAWPYIQSGE
                     ARFVIANWDTSDVRRSQFILRARAIKLTQPLYILLTMAKITDDDQTPSGADDILQRPF
                     RAKDLKNRVGMAERIISLASNLAVAREQLEHQAVFDSLTGFLNRAAFFKQAMGELERA
                     RRTSMPVSLIALDVDHFKVINDSLGRDIGDQVLHVVAQAIREKSRPYDCIGRWAGDEF
                     VLMLSGVIGADAEKVTERIITGVRSSRVVVHNNTPLNIKVSAGIASVARISTSVEVEP
                     LIQQALQAMKRAKEAGGDQVFLIYM"
     gene            26139..26210
                     /locus_tag="PLE39_09560"
     tRNA            26139..26210
                     /locus_tag="PLE39_09560"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26171..26173,aa:Met,seq:cat)
     gene            26247..26915
                     /locus_tag="PLE39_09565"
     CDS             26247..26915
                     /locus_tag="PLE39_09565"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNE68616.1"
                     /translation="MQIQPDTLIRSRRRTIALIIERDGTLTVRAPMRASKAVIEEFIR
                     EKADWITRTRKKLTPILTAPEKQFVDGENFLFLGSSFDLKLVQRQRPALRFENGFTLS
                     IAAQKRGRQVFTNWYKERALEVITERVNHFSARYGFQPKQVKISSAKTRWGSCSANGT
                     LNFTWRLVLSPLSVIDYVVVHELAHLRVRNHSRKFWQVVESIDPDYKTKRKWLREYGE
                     TLYF"
     gene            26917..27666
                     /locus_tag="PLE39_09570"
     CDS             26917..27666
                     /locus_tag="PLE39_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68617.1"
                     /translation="MEEAERLYADFQVKIAGDEVWRPNDLEFLHASITILSNAMGGSE
                     NFKQQLGEVLIERTDTGKHLGLAHRGRIQLSKSTPFSAWSVIHELSHAWDAKHHWMLS
                     VLLQKYTGGFTSRFLSRIKKWFPRQWDAGRHGAEQTPGRYGRKPGVNAFGYFYGDRPS
                     GSNWRFNHKEDFAESLVMYCGWGKNNLLSRTAHGRIERYTLPNGERDPLYGIVDQWSD
                     YARYFYPPEGDYAQTKRWKFFDELIHGKIPT"
BASE COUNT         6620 a         7483 c         6986 g         6528 t
ORIGIN      
        1 ttttatttgc ctcgaaaaac gcttcgagta attcttcctt catttgcttt gcgaaagcag
       61 gctggtctgg atacagatca tggagttctt cgatgttttc gctcaggtcg tacaactcga
      121 aggaatcttc cgcttcgtaa cctgtgtagt aaataagttt gcggtctccc tttcgcaaag
      181 cgaccgtggc cttgttgagg attccgttgg cagggctgag ttttgcttcc acagaataca
      241 aactgcgttc atagtcttct tcaccgccga ggccgggcaa tggctttcca tcagtccacg
      301 cggggagcgg attccccgtc aggtgggcca gcgtggggag aatatccacg gcattggtgg
      361 gggtgtaaat atcgcggcgg gtcttctgcc ccggtgcgga gatcaacaac gggatgtgaa
      421 tgaccgggtc atacaacaac ggcgtggaat gtcccttctc accccgctcg aacatctcgc
      481 catgatcggc agtgaggatg acataactgt tctcaaaaag tccctgactt tcaagcgaat
      541 ccaacaacct gcccaattcc tgatcgaggg aggcaatgta ctcatcgtac aaacgccgcg
      601 ccagattgag atccgcgttt gacgtgccat cggcaaaacg atgatcaggt ttgcgcatgg
      661 gcttcatatc atctcggaag gcattgaaga atttatcggt ggggcggtag ggtgtatgcg
      721 gcggcagcag atgcaggtac gtgaagaacg gcgcgggcag ggatgggatc aacgagatca
      781 aaccgtcaaa cacatcctcc aaccgaaaag tgatcggata gttgagatcg tgaggcaatt
      841 ccttgtagcc atcggtgggc aggtgattgc tttgccacgt gtacaacaat ctttccagag
      901 agcccagcac caaagaaggc ggcgcctccc ccaccctgaa cagaaaatca tccaacgcgc
      961 gcgctgacat gtttttatct ttggggaaat gatcgctcag cagataactc aactcggaaa
     1021 attcgcccga tgaaagcaac acatcaatat cgccttgaaa ttgcgaaagc agcagggtgg
     1081 cccaaaaatt ctgtgggaaa gccaggcggt gatagtcact acccaacacg cggaaaagat
     1141 tattctccac catgctttgc cgaatcgcgc cgcgatgatt gatggcgcga tgcttccagg
     1201 gataggttcc cgtcatcaac gaagccacgc cggggatggt gtagtttccg ctggagttgt
     1261 gcgcgtggta cacatttgca tgttcggcga accgctccat cgcaggcact gtggggcgcg
     1321 gatatccgta cagtgaaaga ttgcgcgcac tcatggcatc gaacaacagc acaatgatat
     1381 tcgggcgcga agcatccaac ccgccaatgc gatgagcaag gctgggcagc aacatggcag
     1441 gagcggcagc cccgatcaac ttcaagaaac tacggcggtc gaatttggac atcgatattc
     1501 cttcttttta tgtgcctttg tgtgcgatgt gcttctcaaa aaatattccg cgtcaacagc
     1561 accaaaatcg agatcgcggt gatcggcatc cagagataca gaaacacggt catgcgattt
     1621 gcaacttctt ccatcgtctt tttcaaaaag ccaacccgac cgaacagaaa aacaagaaat
     1681 gcagtgaccg cagcaaaagc caaaactttt ggaagcaata tcgaaggatg cagaacatcc
     1741 tcgaacaggg tttggttgaa aaccatcaac ccgccgagaa cgagcagggt caaagtactg
     1801 cccttcacca cgaactcatc agaaaaccac gttgcaggca gcaccgcact caatgcaagc
     1861 agaacaagca aaaccaaaag gctttcaaca aaattgaccg ccaccgtata aacataaatg
     1921 cctccaacct gtcccagtgt tgaaaaattg agccagcttg aaagcctcca aaaatactga
     1981 aagagcgacc acacatacac catcatcacg atgacggcat aaacggggat gacctgctcc
     2041 cagcggggga tttttttgac gatgtccaag gggaatattt ccttcacgac ttgcaacgct
     2101 tcacacttaa ccgaaagctg attccgttcc gaggggcatt gccgcgtacc gcaacgttca
     2161 cgttgcccac taccaattca aatccttctc gtacttccag tgcatcagca tctcgcctgc
     2221 aacttctttg aacacagcct tgtcacgttc ggtgaacagc cttgcccaat tgccagcctg
     2281 ccccttcggc aaaaaggacg caatgccttc attgcgtttg gattgccatt cttcatcagg
     2341 gttcgtggac atttcagccc gcagggacga atcaagagaa gggtccacac tttgcacgga
     2401 gaggaatttc cacagtctgc tcatttcatc gaacggctgc gccagcaggt cttcataacg
     2461 cattccaaaa taacggtcat tgaacagtct gcggccttcg ccttccgttt cggtcaggtc
     2521 gttcacccaa tctttggcaa cgcggcggat gaaggtttcg gtgaatatcg aacggcggcc
     2581 atctgaaaat ggaacaggat ccagccgcag gtccgaaatg atgcgtttgt cttccgatgt
     2641 cagaaacctg gattcttcga cgaaattccg aaaacgttcc gagatcaaca cgtcacgtcc
     2701 atcgcggacg atgcaaacca ttttcgcatc ggggtacact gcgtgcatgt cacgcacggc
     2761 ctgcccgtga atggtggaag atgggctttt atcgccgacg atcattttgc cttcgcgggc
     2821 ggcatcgcgc tccatgatga aatctgcggc ggcgcgcagc accagcggag aaaggtcgcg
     2881 gccttgattc cagcggttgg atttgcgggt gagccattct tcagcttcgg gtgaatcgac
     2941 cagcgatttt aacaacggct tgcgggtgaa aaaatgcgcc tgataattgc agtgaatttc
     3001 gggatgcagc cgtgcaaggc gcatgagcag ggttgtccct gaacgggcat ggccgaaaat
     3061 gaaaaattta tcgcgcggaa aaaaggcgcg ggcctcggca acttcctcgg cggtgatggc
     3121 tgggatgggg ttacggtttt tcttttgggg ttcgccgcgc aacagaattt tggcggccag
     3181 tttgaatcgc ttgagcaagg gttgtttctc ctaaccgcct gattttactg gaaatatcgc
     3241 cgccttgaga tatgtggttc agcgtgggtg tgctaaagtt tgtagatggc ctgctccctt
     3301 cgccgtcctc ggcgaaggat ttactcgcgg aacgccgccg aggacggcgg cttgagctga
     3361 ggcccattga gatatgtgat tcagtgccag cttaactttt aattcaaacg gttgtacaat
     3421 gattaatatt attcatcgca aaatttcttt tttttgcgca tttgatttcg gaaaggcggg
     3481 gcgaatgttt cgggtatttt ctttgtctaa aaaacgaccg tttattttta tgtgcggcct
     3541 ccagaccatg ttggttttat ttgggagcac tggatgcatg ccgccctccg ttacaactca
     3601 ggtggctgag gttgccccat tagaggtttg cacacctgtc ccgtcggaaa acgtcaatgt
     3661 gtatcggacg attccacagc catgggcagg cctgatcttt gattatgccc acgccaccct
     3721 ccctgccacc tcccccacac tggacccatt actagccgct gctttccctg caatcacaca
     3781 acttcagggg gcgcagtatg ctgcgttcca aaagttggtc agcgagacaa aacgctggtc
     3841 tgatactgaa acgatcaagt tggatgatgc tgaaaacaaa gtacgcatca cactgacctt
     3901 catcagcccc caactcctgc aggcggtgta cctcaatcag gtgctcaaag atggataccc
     3961 cgccagcggt tttctggaac aacttcaaag tatgttgaat aatgttgccg cacgcgatga
     4021 gttacttttc ctgttgacca tctctccgtt gagcagcatc aaccccatcc atcacaccgt
     4081 tgaactaccg atcgactcca tcgttcttaa taattcggaa aacctgcaag tgagtcacag
     4141 ccatgatgac cacaacctgg cccagccttt cgatacatcg tcagatgcga tctttggata
     4201 ccttgccttc ccattcacac aattagtgaa cagtgagtgc aagtggatcc tgaatccaaa
     4261 attcaatacg aatatcgttg tcaccatgcc gtatcttaaa gtggatggca acaagaacgg
     4321 catatcctat tcctggacaa ttccatacac ctccctgatc aatcagaccg tgactttaga
     4381 tgccctgaat tttaacccgc cgcccgggta tgatttcacc caggtgacgc cgtcatcact
     4441 gccgcccact gacatctcca gcccatcatc gtacgacgag agttttgcaa aattcatctg
     4501 gaaacagatc acaggaagtt attaacccat gaaaaccatt ttcacctttg tgccttcaaa
     4561 aacgcatgaa tttccagacc accctgagcg gcccgggcgt ttggatgtac tggaaccgct
     4621 gcttgcttca gtggatgcag aaagaatcaa ggcggtccct gccgctcccg aacaaattgc
     4681 attggcgcac aagccgcagt tggttaaagc cattgcggag gtctgcacac gcgggccggg
     4741 catcattgac catgcgccga ccttcatcac gcagacctcg tacgaagatg ccttgctggc
     4801 cgcgggaggt gtggtcactt gcacgcgcgc agtgctcaac ggcgaggcgc aaaatgcctt
     4861 tgccattgtc cgcccgccgg ggcatcatgc cgaacccgac cgcgcaatgg gtttttgcat
     4921 tttcaacaac atcgccatcg cggcacgtga tgcgctggca aacggcctgc aacgtgtgat
     4981 ggtcattgat tacgacgcgc atcacggcaa tggcacgcag gcagtgtgtg tggatgatga
     5041 ccgttttgga ttcatttcga ctcatcagtg gggaatttac ccgggcaccg gctggatcaa
     5101 cgacgcgcct catgcgaagg gacggctggt caatgtaccc ttgcatgagt ttgcaggcga
     5161 cggaacgttc gcgcgcgtgg ctgacgagat tttcaagccc atcgtggaat ccttccgccc
     5221 gcagatgctg ttgatttcgg cgggcttcga cgcgcattgg aatgacccga tcacttcgct
     5281 gggactttcc acccgcgggt attacgagat ctcgaaaaaa cttgttgcgc ttgcaaatga
     5341 acattgcggc ggcaaaatcg tcttcgtttt ggagggcggg tacgatccgc acaatgtggc
     5401 caacggggcg atggcggtct tcgacgcatt gaccaactct ccgttgaata aagatgcggg
     5461 cgatccgtcg ccttatcccg attcagatca cgagtctcgc atccacgata ttttgaaatg
     5521 gcatggcatc gcacagtaaa aaggttttgt tgtagaatcg cttccgtaaa aaggagcgag
     5581 agatgcaaag aaaacaaagc aaaggttttg ttgagctgga atgggagtgc ccaaactgcc
     5641 acagcgccaa caaaggttca aagaaaatct gtgaaagctg cggcgcaccc caacccgaag
     5701 atgtaaagtt ccaacgggca gcggatgaga agatcatcac cgatgagaag gtggtcagtg
     5761 cggccagtgc aggcgcagac gttcattgcg gattctgcgg tacgcgcaac cctgccacgg
     5821 caaccaattg ttcccaatgc ggtgcggacc taaaagaagg caaggccagg caggcgggac
     5881 aggttttgca agcggcgcct gaatcagcga agatcattac atgcaaaagc tgcggcacgg
     5941 aaaaccccgg cggggaacgt ctgtgcaata aatgcggtgc gcctttgccc cgtgctgagt
     6001 ctgttgccgc caagcctgca gcagccgttg cagcgaattc aggtaccagt tcggctgctg
     6061 caccgaaggc tcaaccgaaa aagacgaatt ggcttttgtt tggcgccatt ggcgcgtttt
     6121 tgatggtgtg ctgcatcgcg atctttgcga tgtttgtgat tccctccaag tcggtgcagg
     6181 gaacggtggc cgatgtgtat tggcagactt ccgtgccgct gcaggaggtt cgcgcggtgg
     6241 attattccaa tgagcgcgga aacccgccag caggcgcata caatgtttcc tgtcacactg
     6301 aaagccagga aatctgcgaa gacaagaccg tggatcgagg aaatggtttc gcagaggtca
     6361 ttacagaatg ccacaccgag agtcaggatt attgcagtta caccgtggat gagtggacga
     6421 ccattcaatc gtacgatcta agcggcagtg acttgttccc agagtatgcc cagcccagtg
     6481 tattcaatgg gcagcggttg ggcaatgctt cggcgacgtt gacggtcact tttgatacgc
     6541 caaagggaac catcacctac accccaggca cggtcagtga gtttcaacag ttccagatcg
     6601 gtagtacgtg gaccttgaaa ctaaatgccc tgggcggggt cttgagtgta ggaaaataag
     6661 gggcgaaaaa tgacagaccg ggccgaagac tcgctgaaaa atgatgtttt gtacacagat
     6721 gaccttgcgc tcaatcgcaa tggacagtta tctgaaacgc aaaagaaacg tctgtggccc
     6781 ggtgctctgt tctggcttat gcttgcaggc atcaatgtaa tattcctgct gctcctgagt
     6841 ttctttcaat tgcgctatca aactgtaggt tgcgggtatc tggggttatt cgtgatcgcg
     6901 atcttctcca taaggcaatg catattaaat tccgtgccat acagggaaga tattaagaat
     6961 gggatcgtaa aaacggcttc agggcaggca ttcaagcggg ctgttcttca aagcttcagc
     7021 aaaggggttt atttgggtaa ctgttctgtg agagtgaaca acaaggtatt tacgatctcg
     7081 ccgcattttt acaactggat ggttgacgag ggttattacc gaatatattt cactcccaaa
     7141 acaaaaagag tggttaatat cgaagagttg taaagttacc aaaacggcat ggacacgttt
     7201 cattaaaact cccgtctcat tcgggagttt ttgtctttat aatcaactta tgaatcattc
     7261 agtggcaatc atcggtggcg ggcctgcggg gttaatggcg gcggaagttt tgagcggaca
     7321 tggcatgcag gtggatgtgt acgattccat gccttcgctc gggcggaagt tgttgatggc
     7381 gggcaaaagc ggactgaatc ttacccattc tgagcccttc gaaaaattcg ttttacgcta
     7441 tggcagtaaa aaacaggaag tggaaacctg gttgaacgat ttcacacccg atgacctgcg
     7501 cgaatgggca cgcgggcttg gggtggaaac gttcgtcggc acttcggggc gggtttttcc
     7561 aaaagagatg aaggccagtc cgctgctgcg ggcatggtta aaacgattaa ccgcggcggg
     7621 aatcaatttt catttgcggc acaggtggaa tggagcaatc gccgccgggg agggcggcgg
     7681 gagcattgtt aaatttgaaa cgcccgacgg aatcaaaacg gtccatgcca atgcggtgat
     7741 cctggcgctt ggcggcggca gttgggcaag actcggctcg gatggcgcat gggttgaatg
     7801 gctcgatcag gccggggtta aggtggaagc gttgaaacca tccaattgtg gattcgatgt
     7861 ggcgtggagt ctgcattttc gggagaagtt cgatggtcag ccgttgaaat cggtgaccgt
     7921 ttcattcggt gattttcacc agcagggcga attcattgtg accagggagg gtgtggaggg
     7981 aagtttgatc tacgcggctt cggcgctgct gcgcgatgag atcgcacaga atggtcgtgc
     8041 aatactggaa ttggatatgt caccagatcg tccgcttgaa tggctggtcg aaagattatc
     8101 caaaccgcgt ggttcgcgtt cgatgtcgag tcatcttgaa aagacagtgg gattaaaagg
     8161 cgtgaaggcg ggtttgctgt acgaatttat ttcgaaggat gattttgcaa acacagagcg
     8221 gttggcattt tttatcaaac acctgcccat cccgctgatc gcaacccatc cactggatga
     8281 ggcgatcagt tccgctggcg gtgtgagctt taaagcggtg gatgaaaatt tgatgttgca
     8341 caaactgcca ggtgtttttt gtgctggcga aatgctcgat tgggaagcac caacaggcgg
     8401 atatttattg accgcctgtt tcgcaagcgg acgggttgca gggttgggcg cgttgaagtg
     8461 gagtaacgcg ggataatatc ccgcgctacg gaaccccatg aagaatcttg attaccgcga
     8521 ctattaccgt agaaatcttc cacactttca gccgcgtggc gcaaccttca tcgtcaattt
     8581 tcggctggct gggtccttgc ctgttgaagt tgtgaaaatg ctaaaagcag aatcagagga
     8641 aattgaaaag tcgctgttga gaatcgaaga tcatacagag aggtatcacg tcaaagacag
     8701 ggaacagctc aggctatttt cgaaatggga tgaagcgctc cacaacagtc aagccgggcc
     8761 attttggttg aaagatgagc gcattgctca aatcgtggtg aactcgattc tgtatcacga
     8821 cgaaaactgg ttcgacgtgc tggcgtattg catcatgccg aatcatgttc atttgatatt
     8881 ggcgccccgc gaaaatgccg acgccgattt cagccttgcg aaaatcatgc acaacatcaa
     8941 aagaaactcc gccaaccatg ccaacaagat tttagaaaga acaggcgcat tttggcagca
     9001 tgaaagttat gatcgctttg cccgcaacga agccgagctt gagagaatga tcaaatatgt
     9061 gctgtacaac cctgtcaagg cagggctggt ggatgattgg aaaaaatgga aatggtcgta
     9121 ttgcaaattt gaaatgtagt gcgagataat atctcgcact acattaaaag ggatccaacc
     9181 atgcatgaag acatattgaa aatacaacaa ttgatcgcac gctttgccaa tagtttcgat
     9241 gtgaaagatt gggatggctt acaggcctgt ttcaccgaat cgctctacac cgattactct
     9301 gacttgcgcg gcacaccacc tgagaccatt tcagccagtg agtatgtcag gctccgccgt
     9361 gagtcactcg atcatttgaa actgcaccat ttggtcagca actacgagat caccttccca
     9421 ggtttcgatg ttgccgcctg ccgcgcctcc atggtggttt ggagaagatc tgaggcggaa
     9481 gatttcacga cccactgcat atacgacttc caacttgcaa ggcagcgggg aacatggaag
     9541 atcagcggca tcacacaaaa gattttgtgg aatgatggaa atgcatccat tcataaagga
     9601 gccaaaaact agccatgtat cgaaaaggct ggcgatatta tcgcgacgca accctgcttt
     9661 ggatattgtt ctttgtcgtt cttatgatcg gcggctacgc cctggaacac ccgctgtatt
     9721 ggatcctggc tttgttcgta tgggtggtcc gctcggcgtt tgggaatgcc tttcaatttg
     9781 cgcatccatc ccgtcgcttc aactgggaca gcagatccaa cctggtgttc aaggaagtca
     9841 cgttcaaatc acgtgatggt ctcaccctct tcgggcgttt tcatgctggc agaactcgcg
     9901 ccaccattat cttgatccac gggttgggtg gtgcaggtac cgatatgctg gtccatgcct
     9961 cgtttctggt cgatgcaggc tacggcgtgt tcatgatcga tctgcgcgct cacggcagca
    10021 gcgacggtga cacatccacc tatgggctgc gtgaagcaga tgatgttgca ggcgcggtgg
    10081 attacctgct cagccgcatg gacgtcaacg gggcaaagat cggtgtgctg ggaatatcgc
    10141 tcggcgcaca agccgctctg cgtggtgcgc tgaaaacgga gaatattcgc gccctcgttt
    10201 tggatggtct cagccctgcc cgcctatctg accacggcgg cagccccaaa tccatacaac
    10261 gctggctcaa ctatcctttt aactggctgt attatcggat ttatgaatac atgatcggcg
    10321 gcagggatgc gggtgttctg gaggtgatcg gacagatcgc gcctcgcccc atcctgttaa
    10381 ttgccagcgg tttaagagac atttacttca gccgattatt ttacgaagcc gccaaagacc
    10441 cccgcgcgtt atgggaaatt cctgatggca ttcacggtgt ggcgcttgct ggcaatcccc
    10501 aggaatacat tgaccgtgtg gtcggctttt tcaaccagac cctgctatga cggcctgccc
    10561 tgaatgtggc gcaacgaact gtgaatcgcg gttccatgaa ttccttgcct tggaattcag
    10621 caacgccgcc tacggcaatg tccaccatct gaccgtggcg gcttacatgc tccaacattc
    10681 cagcaaatta acccgcgaag gctggcgtta tgagcgtgag ctactgagag catttttggt
    10741 ggaaaataaa acgcccgcct ttatccgcag gcaaaacagg gattgggtgg atggcggcaa
    10801 acgaactttc aagatcaaat caaaagacgg atccccattt atcaatagat tcgcgtggac
    10861 aaagacgatt ctcaatgtcc gcgccgaaaa tgcggaagtg tattgcgcag atataaccac
    10921 atgggcgggt gcggtcttga aagatgcaga agaaataaaa ctggaaatat gaggacatcc
    10981 aagacgaacg gctcattcca cggccacaaa gccatcagga atcctttgtg acttcgcagc
    11041 gattaaagct cgttctgtta gccgctccct cataatatta tctcgctcca catccctatg
    11101 ataaacttac caaccatgaa acgcgcgcta caaacttcat tcatcatttg gacgattctt
    11161 tcggcattgg ttctcgccag tgtgattgtc atctacctcc gcaacggcga aacgggactc
    11221 aatctcttca tccagcaatg gccgctttcc aatttgaccg tgacgctgga agccaccgcc
    11281 tacacctggt tgtgcgcagg cgcgatcctg tacctgctgg cgaatgacaa agttaacgcg
    11341 gaaaatgcct ttttcgtggc gggatttttc atcgtcattt tgctctacct gaatgtgttg
    11401 cgcgaacgct tccgctatgg cgattatcag tactatctcg aagccgccac cgcgttgatg
    11461 aataaccaac ccctgccaga cacttatctt tacctgccgc tttgggccac cctgttgcaa
    11521 ttcatcgttc cgcttggcga ccaggggatg ctggcgatct tgtggacggt caacatcatc
    11581 atgctgggtt ccttctacat tttgctcgtg cgggtccttg aacactacgg atattccaac
    11641 cgtttcgcgg tgatcattgc cgttttgttc atgtccatta atacgccgct gcatcgaagc
    11701 ctgggatatg tgcaggtcaa tttgatcgcc atggtgttga tcatggcaag cctgctcact
    11761 ttccgtaaaa atgacctcgt ctccgcgctg ctgctggcgc tagcagtcca cctcaaaacc
    11821 tcgcccgccg cgcttgcgct tgccttcctg ctcgagcgca actggaaatg gctggcatgg
    11881 ttcgcactga ccttcatcct gattgcgctc atccctgttg cacttgaagg cacacgcccc
    11941 tacttcgatt acctgaccaa catcaacatc ctcacccaca tcaccgacac caacttccac
    12001 gacacttcgt tcgattcatt tttccgattc ttcaacccct tccttgggat caacctcgaa
    12061 gccacacgcc tgatcacaat gggtgcgaag gcgctgcttg ggctggcggc catctacgtc
    12121 atggcgcaaa atatccgcag ccacaccttc gtcagccgcg aaacgcaaaa tcatattctg
    12181 ctcaacgcca tcccatcgtt gttcatcgtc atgaccctca cctcccccat cgtttgggat
    12241 catcacggca tcttcaccac ccttgcattt ttgctcctgc ttcattgcat cgaaacccct
    12301 gcactgtgga tgtggttcgg gtttgcctac ttcctcgaat tcatcctgcc ttccttcgat
    12361 ttcttcccct ggtcatttgg ccgcctgatc gcaccgctga tcgtcctctg cctgatgtgg
    12421 tatgtcacac gcagcaaaca aagcccatcc ctccttgttt cggcaaatcg ctggtttgag
    12481 aatctgaaat tccaaatctc ccaatgaaac aacaacgccc aattctgatt ctgcttgccg
    12541 tgagcctgct cattggcatt ttcaccttga acggctatgg cgatagttgg gacgatctct
    12601 cacttcgcaa atacgctgcc aaatcgcttg aagcctacgg cacatgggtc gcacgcggcg
    12661 aagtacagat caccacagaa gacctcggca gttatggccc atcctatgtg atggcagtgg
    12721 ccctgctttc gcaggacaat cccgatctcc gtcacttcat ttattttgtc acatttcttg
    12781 caggtgcctg ggcgttttac atgctcggct tgcgctggct ctcgcagcca gccgccctcg
    12841 gcgcaaccct gctttacctc acccaacccg ttctctgggg gcacgccttt ttcaacccca
    12901 aagacacccc ttttctctcc ctctttcttc tttcaattta ttttggatta aaacttttcg
    12961 acgaatcgcc caacccgatt cagggatcgg accgaagaac cgcgcttctc accgccctgt
    13021 ggcttggttc tgttttctcg ctctttctcc tcaccgatac atttcacacc gccattgtga
    13081 atcttgtcca cgctgcaaaa gcgggcgaaa ccaacatcat ctccctcacc gcatcagata
    13141 tccgaactgc cgcgcctgaa ctctacatcc aaaaatattt caccctcttc ctccgactcc
    13201 gcgccgtttt ctttcttctg tcttctttct tctttttatt tcacaagtct tttcgctctc
    13261 ttcatccttc tgccttcacg cgtagcgttt atccttcggt cttcctcggt ttcacctcct
    13321 ccctgcgtgt tctcggtccc tttgcggggt tgatcgtggc atactacgca ctccgcaagc
    13381 acggacgaaa atccattccc actctcatcg cctacgctgt gaccgccatc atcatcatgt
    13441 atctcacctg gccctacctg tggacaaatc cgctcggcaa tttcatcgcc agcatcaaaa
    13501 ccatgtcgct ctacccctgg agcggacagg tgcttttcaa cggccagcag ttcgcatcca
    13561 ctgaattacc tgcctcctat ctgcctgtgt tgctcggcct tcagttgacc gaaccgctct
    13621 gggcgctgac tctgatcggg gtggtcgtgg caggcatcag attaagagag aagcgtgaac
    13681 tgatcgagtt gagcatcctg tggttaattc ttccgctgtt cgggttcatc gtcatgcgga
    13741 cggcgttgta cgataacttc aggcaggtct tctttatttt gccgccgctg ttctggctgg
    13801 caggggtcgc gatcgaacaa gtcaaacgcc ctgcatttca aatggccttg atcgttttgg
    13861 cagcgctgcc tgggattgtg gatgggattc gtttgcatcc ctacgaatat atttattaca
    13921 atcgcttcat cggcggtgtg gatggtgcgc agggccgctt tgaattggat tactggggcg
    13981 cttcgtaccg cgaggcagcg gagtttgtga acactaccgc gcccgcaaat gcaaccgtat
    14041 ggatcgaagg tccttcacag ttgttcgagt tgtatgcgcg tgaagacctg aagttgtatt
    14101 ccacacacga agcagaccgc gccgaccatt acgattttgc ggtggcgctc acccgcgatc
    14161 acatggatca ggcttcattc ccaaacgcgg aagtgatcta cagaatcatg cgcggcaggg
    14221 ctgtgctggc tgtggtcaag actccgtaac gcaggttgta cgcgttatac gcgtaccgca
    14281 aacttcagtt tgcattaccg cattccattg aaaagannnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14401 nnnnnnnnnn nnnnnncatt gaaaagagaa aatattatga acttccttga attgctttca
    14461 caaaaaacgg tcgtcgccga tggcgcgatg gggacaatgc tgcacgcgca tggcgtggat
    14521 tttggaaaat gtttcgatga attgaatctc accaaccctg ccgccgtggc ggatattcac
    14581 cgcgagtata tcgaagcagg tgcagaattg atcatcacca acacctttgg agcgaaccgc
    14641 tacaaattga aaaaaaacgg attggcagac aaggtaaaag agatcaacca cgcaggcgtg
    14701 gaactggcaa agaggatcgt cgctgcttcg ttcaaggaag tgctgatcgc aggggatgtt
    14761 ggtccgcttg gggtgaggat cgctccatat ggcagggtca agcctgagga ggcgcgcgaa
    14821 gcgttcgcgg agcagatccg cgcgttggca caggcgggcg ttgacctgat cgtgatcgaa
    14881 acgatgagcg atctttatga aattcaagaa gcgatcaaag ccgcgaaaca ggcatgtact
    14941 ttgcctgtgg tggcttcggt gacgttcacc cgcgatgacc gcaccctgct tggcgatgac
    15001 ccgatgaagg tagcgcgcgc attgcacggg gcgggcgcgg atgtgatcgg ggtgaattgt
    15061 tcgggcgggc cttctcaact gctgcggatt ctcaaacaga tgcggcaggc tgtaaccgac
    15121 cgctccgcaa agttttgggt gaagccaaac gcaggctggc ccgaacaggt gggcggcaga
    15181 attatgtacc ccgctgatgc cgattacttt ggcgattacg cgctggcttt ccgtgaagcg
    15241 ggcgcgaaca ttgtgggcgg ttgctgcggc accaccccgc agcacatcgc cgcgatgaaa
    15301 aaagcatttg caacaacacc tgttcaaatc tcggaaatgt gggaagcctc tgaggttttg
    15361 gaaacagaat ctcaagatgt ggaacaacca acccaattgg cacaaaaact tgccgctggg
    15421 aaattttcga tctgcgtgga gatggacccg ccgcgcggac tctcgactca caaattgctg
    15481 gcgggggcgt ccctgctgta cgatgcaggc gcagacgtga tcaacgtggc cgattcgcca
    15541 atggcgcgca tgcgcatgtc ggcatgggcg gtgtgcgatc ttgtccagcg ccagatcggg
    15601 gtggagacca ctctgcactt ccccacccgt gggcgcaacc tcctgcgcgt gcaaggtgac
    15661 ctgctcgctt ctcatgcgat cgggttgcga aatatcttcg ttgtgatggg tgacccgact
    15721 tcgatcgggg attatcccga agcgacagac aactacgatc ttgtcccctc gggcttgatc
    15781 aaactcatca agcagggatt caacgcgggg gtggatcact caggcacaag catcggccag
    15841 ccgaccaatt tctttgtggg cgcggcgttg aatctgtgcc cgcaggatat ggataccgag
    15901 gtcaagaatc tgcatcgcaa gatcaaagca ggcgccgatt tcttcctcac ccagccgatc
    15961 taccgcgccg atgacggacc gaaattgatc gaggcttacg aagccaaaca cggcaaattg
    16021 cagcagcccg ttctggcggg cattctgccg ctggtcagcg caaaacacgc caactttttg
    16081 cacaacgaag tgcctggcat tttcatccct gaagaagcgc ggaagaagat cgaagccgcg
    16141 ggcgatgcag agcgcggagt caagatcggg gtggagttgg ctgtcgaact gatcgagcag
    16201 atcaagggtt gggcgaacgg catttacgtc atgccgcaat tccatcgcta cgacatggtc
    16261 gcggagatca tcgaaaagat tcgctagtac cgcattgcag actgaagtct gcggcacaca
    16321 acaagatata ttcttatgtg gaaaaaaact cttcaaaaaa taattctcat ccttctcgtt
    16381 ttgctcggca taaaaacggg cgttcagggt ctggcctcca ccatcaacat gcccagccac
    16441 gatgtttctg ttgcgaagtg ggatgaacgc gccgccgcat tgaaggatgc cctgcccttc
    16501 aaacgcggat ttgtcggcta catctccgac atagacatcc ccaacgtgga atatgaccgc
    16561 cagaaccttg tcggtgaatt cacattgact caattttcgc ttgcgcccat cgttttgatc
    16621 cagggatcgg acgatgaaga atggaacatc ctcagcctga gccagcaggc atacgaaatt
    16681 tggagcgcgg aaaatgccgc acaatatgaa gtgatcaaat ttggcggcaa gttttatctt
    16741 gcgcacaagg tgaccccatg accgccgcac tcagcctgct ttccttcgcc ctgcccatgc
    16801 tgacagggat tttgctcatc catgccttgt gggcggagaa aaacaatttc ttcgattggg
    16861 ttttaaaact ctcgctggga acaggcattg ggctgggaat ttcctccctg ctgtattttg
    16921 tttatcttct ggcttttgcg ggcaagccct ggtttttatt tgtcgaggtt ttgaccttcg
    16981 gtgccgtcat catcgcaaca agaaaatatc tccgtgtctc tgtgtcgctg tggttcaagc
    17041 ccacccgcgc acaaacatgg gcgctcatcc ttgcaggtat tgtctttttc accaccgtcg
    17101 ctggcatcct caactactcg cgccaacgcg ccctcggcga ttgggatgca tggatgatct
    17161 acaaccgagc cgcacgtttt ctttttcgcg accaggccgc gtggcaggag tcatttccgc
    17221 ctgagatgag cgtcatcttc catgccgatt atccgctatt gcttgcatcg aataatgccg
    17281 cccgctggga cattctcaac aaagagactc cctacgtgcc gatgttccaa agtgtgttat
    17341 tcgccttcgc cgccttggga ttgatcttcg gcgcgctggg acgattcaca tccgtcggtc
    17401 aagccagcct cggcttgatt ctgcttgcgg gagttccctt ctacctttta gagggcggcc
    17461 gccaaaccgc agaccttcca ctggcgtttt acttccttgc cacagttgtc ttcatctttg
    17521 catattacga agaaaaaaga ttctccctgc tgttctttgc agggtttacc gccgccctcg
    17581 ccgcatggac aaagaacgaa ggcctgctat ttttgctcgt tgcgggtgca gtgatcctcc
    17641 tcctccgtac cgcagacttc agtctgcgca tttggcacaa acaggtattt caatccttcc
    17701 tccccttctt tgctggcgcc ctcctcccgt tgtctgttgc gctgtatttc aaagtggcgc
    17761 tcgcgcctgc cagtgaattt ctcagcggcg gcctgaccaa gatcctgcaa gacatcgccg
    17821 acccagcccg ccacggaatc atcctctctt atttcaaaaa catcttcctc ttcagcaacg
    17881 gctggtatcg tgtcggcatc ctgccgatct tgtgcgttta ctttttggtc tttcacaccc
    17941 gcaaggctca agggaacaac cccgtcttcc ccggctttgc catattcacc ctgcaaatta
    18001 tcgggtatta tgccttttac ctgatctcgc cctacgaact ggattggcat ctcagttcct
    18061 ccattgaccg ccttttcatc catgtgtatc ccaccatttt gtttgtcacc ctcgccgcga
    18121 gtcagacccc tgaaacgatt tttgagtctg atgtctaaaa aaatggatcg cagactttag
    18181 tctgcgcctt cttcaagcaa agcggactga agtccgcatt ccgatataaa aatttttcac
    18241 aggtacccac atgctcctca ctattgacgt aggcaacacc aaccttaccc tcggtcttta
    18301 cgaaggcgaa caacttggcg cacactggcg ccttgccacc gaccacaacc gcatgcccga
    18361 tgaatacggc ttgcagtttt taggcctgct gcaaaacgct ggcaaaacat taaaagacat
    18421 tcatggcatt tcactggcgt ccgttgtgcc gccgctcacg gcgcgtgtca ttcaagcatg
    18481 ccgcgaatat ctcaggcaag aaccgctcat tgtggatgca ggcgtgaaaa cggggatcaa
    18541 agtccgttat gaagacccga aagcagtcgg cgcggatcgc atctgtgatg cggtggcggt
    18601 gatgaaactc ttcggcggtc ccgcctgcgt ggtggatttt ggaaccgcca ccaccttcaa
    18661 cgccgtcacc aaggatggtc aatatctcgg cggcgcgatc accgctgggg tcaacctcgc
    18721 cgcagaaggc ttgttcacac acgccgccaa actcccgcgc attgacttgc agcgcccgcc
    18781 ttccgtcatc ggacgcaata ccgtccacgc catgcaatcg gggttactgt ttgggtatgt
    18841 gagcatggtc gagggtatgg tggcaagatt caggtccgaa cttggaagcg atatgaaagt
    18901 catcgctacg ggcggcatgg ccgaagtcat cgcaaaggaa acacaggtcg tggatcatat
    18961 cgcgccgtgg ctcacactcg acggattaag aatcatctgg gaactcaatc aataggaatc
    19021 gaatgaaaaa aatatttgcc ttgtttttgg ggatgtctat tttattgtct ggatgcggaa
    19081 ctggattatt cgccgaacca acttcaacgc cgaccatgac tttcacgcct ccccctccga
    19141 ccgaagcgga gcgtgctaca ccaacccaaa cctttaccat caccccgaca cgcttcgcga
    19201 cggcttcgcc cagccccaca cccacctggg tgcatcaagg ccctgaaagt gtggaggtgc
    19261 ccatcctgct ttatcactgg atcgcggttt cacccgatga agggaccaat tacaaaagcc
    19321 cgtattatgt gaaacccgaa gtcttcgatg cagagatgaa actactgcac gactggggct
    19381 acaccaccat caccaccgaa atgctgatca aagccattcg cgaaggcgcc gatttaccgc
    19441 cgcgccccgt catcatcacc tttgacgatg gtcacatgaa caattacacc aacgccttcc
    19501 ccatcatgca gaaatacggc ttcactggtg tgctgtatct tgttgccaat tatctggatg
    19561 ccgaagatta cctgaccgtg gaccagatca aggaaatggc cgccgcaggc tgggagatcg
    19621 gcagtcattc tgtgagccat tcagacctga cttcgctcga acccgaacgt cagcgttttg
    19681 aggtggtgga ttcacgcgcc atgttggaat ccaaactggg ggtgcctgtc ctcaccattg
    19741 cctatccatt tggcgtgagc aacagcagtg tgatcgacta tgcccatttc gcaggttaca
    19801 tcggcggcat ggggttgggc tacacccacg atcaaggctt gaccaatctt ttcaatttac
    19861 aacgccgcga tgtgaaaggg acttatgacg tgaagcaatt cgcgtccttc ctcccctggc
    19921 agggcgatgc catcttttta cccaccgaca ctcccactcc caccatgacc ccaacgcgca
    19981 cacccatccc aacctacacg tggatcgtgc cgagtacgtc aacaccgtaa tttataagct
    20041 agagccttta ataaggaact tggtcggcaa atcatcttta tcttgccaag ttcttgatcg
    20101 agctagaaaa ctttttgaga gtgtttgaaa actcagccac aaagtctcga agacacaaaa
    20161 acacgaagtt tttttctttg agtcttggtg cctttgtgtc ttcgtggtga aaaggtgggc
    20221 atttcaaaca ctttctgagg gtcttaattc atttctctcc aaaccgcaac aagaccacgc
    20281 ccatcaaaac caacgcgccg ccgaatagct gcatgcctgt cagcacttca ttcaaaagga
    20341 aatatgccca aatggcggtg aaggcaggtt caagggtggc gatcaaattg gaagtggtcg
    20401 gagaaagata acggatgctc agggtgtaaa gcccaaatcc gccgagtgtg ggggccacgc
    20461 cgaggaagaa caaccatccc cacccgctga ccgaatcccc gagccagagc aggtctgcaa
    20521 atgcgccttt gcccgtcagg aacaggtcat ttcccaaatt gaagaaaaat aaaaagacgg
    20581 tcgcccatgc gaagctgtag agcagggctg tccatgaatt gatgtgggta tctgaggcgt
    20641 gtttgccttc gaggttgtaa attgcaaaca gcagacccga gagcagaccg aagataatcc
    20701 ccagcggatt cagattccat gcggaggggt cgtacgcgcc tgaaacaaaa atgatgcccg
    20761 ccaggctcag cagaatggaa acgatcttga tgcggctgaa ctgctctttc aacacaatgc
    20821 ggctcaggat ggcggtcatt gccggggagg aaaatgccat taccgtggca acagccgccc
    20881 cgttgtattg aaccgagaag gtccacatcg agttgaagat cgcgagggtc aatccatacc
    20941 agaccatgaa gccccaatgt ttacggtcca gttgaaagag cggacggctg aacaccagca
    21001 agccgaccgc catgccaaac gaaacgaaca gatcacgcca gaaagccagc accagcgaag
    21061 gcagggaata gtccctgctc aggtagctga tcaaaatccc tgtggtggac caaatgatcg
    21121 tggccgtaag cgcaatgaca tatccgcgtg agagttgtga cgatgatttc atattcgctc
    21181 caaaaaaaga cagccacctt caaaaagcag gtgactgtca gaagtgtgcc cccacgggaa
    21241 ttcgaatccc ggtttaagcc ttgagagggc ttcgtcctag gccgctagac gatgggggct
    21301 tgcttggcgg gttgcattgt atcactgcaa attttacccg tcaacaagtt atgaacaaac
    21361 aatcattagt ataatcaatc aaccattgtc tcaacaaagg catccactat gaccttcccc
    21421 tcttttctaa aactcacacc cgaatatcgc gattacgtct ggggcggaga aaaactgcgc
    21481 cccgggcact cccccactgc cgaagcgtgg gtggtctatg aaaatgaccg catcgaatca
    21541 ggcgcgctgg cgggcaagac attgagcgaa gccgcaaatg aatttggcgc ggccttgctc
    21601 ggcgaaaaag ttttcgcgcg cacgggcaca cgctttccgc ttttggtcaa attgctagat
    21661 tgcgcgcaat ggctttcggt gcaggtccac cccaacgacg agcaagctgt tcaactcgaa
    21721 ggccccgggc aatttggaaa gaccgaagcc tggcatgtgc tcgaagccca acccgattca
    21781 aaattgatcg caggcattca gcccggggca agtgcggctg aaattgctga ggccattcgc
    21841 aacggcacga tcaccgaata ttcacagtat gccagcgtac agcagggcga tacggtcttc
    21901 atgcccgcag gcacactcca tgcgcttggt ccgggcctgc tcatttacga agtgcagcaa
    21961 acctccgatt ggacgtaccg cgtctacgat tggggacgtc cgcaaactga aacgcgccgc
    22021 ctgcatattg ataaatccat cgcggtcacc cgtcacgacc tcgccgcgcc gatcatttca
    22081 gcaccaccct ctggcgatgg gacacggcac atcctcaccc aatgcgaata cttcacgctc
    22141 gaattaattt ccgcatccac gcggaccgtt gaactggata cccaaaaaga atcattccac
    22201 gcgctgacgg tgatcgaagg ccgcgccgaa cttgtctctg caaccgaaac agtgaccctc
    22261 gaaaaattcc aaacggcgct ggtccctgcc agtttgggaa gctaccagct tcgcccgttg
    22321 gaagaagaag cgtgccgtgt tttaaaatcc agtgtctgat taccttacac tttcaaaatc
    22381 gggacgctga cgaacgcaga aaacgcagat tttttctttt tgaatcagcg aaaatcagcg
    22441 tttttctgcg tccaaaaagt tttgtgcaag gtaatcagat ccagcgttta atttttcagt
    22501 ccactctcat cagggctgag cgtttggtca ggtcaatttc attcatggcc atcactctga
    22561 aactgattcc ctttccgcaa attaaaaatc acaagcggct gtttttttgt atcgccccat
    22621 aaaaactcaa aggtaaacga tcatgacaat caacgacaaa accgtaacca tcatcggcgc
    22681 gggcatggct ggtctttccg ctgcgcttga cctgcatcgc gcaggctggc aggtcaccgt
    22741 gctcgaagcg cgaaaccgcg tcggcggacg ggtgcatact gtacgaagtt tttccaacgg
    22801 gttgaccgct gaaggcggcg gcgagtacat tgacgaagat catacccgca tgatttcgct
    22861 cgcccgggaa ttcaatcttt cattgggtaa agtgggaagc tggcaatcac aaaacggcga
    22921 ttggggtgcg ttcgaaggca aagctggctc aatcgcagac tccacgctgt ggggcaccaa
    22981 ccttgccagt gaaattcaca acatgtggca ggccgttgca gaactgggga aggaagtgaa
    23041 cgatccttcc aacccgcaaa ctgcccgccg cgccgaagaa ctggatcatc gccccgcatc
    23101 cgagtggatc gccgcccaac ctgtgcatcc gctggcacgc aaggccttta tccagcacat
    23161 ccgctcagaa tacacgtgtg aacccgaagc cttctccctg cttgaccttg cccgtaatgc
    23221 ctccttctat tacagtgacg cagaagcatg gccgcagacc caccgcatca tcggcggaaa
    23281 cgaccaattg ccgcaagcca tggccgcccg cctgcctgat gtgcggttaa acgcagaggt
    23341 ggtttccatc aacctccaac cgcaaaaagt aaccgtagcc tataaacagg gcgaggtgta
    23401 ccacacgatc aattcagtct ttgccattct ggccattccg ctcaccacag cccgcctgat
    23461 cgatttcaaa ggatcgctgc catccgcaca tcaaaagatg gtgaatgaaa tttcctacgg
    23521 caaagtcacc aaagtgatga tcgaatatcg taaccgcttc tggaaggaca aaaaatggaa
    23581 tggccgcctg accaccgacc tacccatcgt cctgacatgg gatgccacca gtcatctcga
    23641 aggcgagcgg ggcatcctca ctgcctacac tggcggcggc ccgggcgcat accttaccgg
    23701 cctatctgac gaagaacgta cgaaaaccgc cattgcggtc attgaaaaaa tattccccgg
    23761 atcatcagac ctgatcgaaa gcaccgccac cattgcatgg ggcaacgaac cgttcagccg
    23821 atgttcctac atggcacttg cccccggcga ggtgactgcc cattggaaaa ccctgttcag
    23881 ccccgctggc cgtttgcact ttgcaggtga acatgcaaca ccctatcagg gatacatgga
    23941 aggcgcagtg gaaagcggtc agcgcgccgc gaaacatatt atggagagtg tatgagtgaa
    24001 tcaagcaagg cattttggga aggcgtacgc gccgaattcc cattattgat cggcgttttc
    24061 ccgtttggca tgatctatgg cgctctggcg ctcaatgccg ggttatccac gctggcatcg
    24121 caaatgatgt cgtccatcgt atttgcaggc tcgtcgcaat tcgtcaccgc gcaattggtg
    24181 catgatgccg cccctggcct ggtcatcatt gtcaccattg cagtggtcaa ccttcgtcac
    24241 atgctgtaca gcgcatcgct tgcgccatac ctgaaaaacc tttcactcca atggaaggcc
    24301 ctgctttcct acctgctgac agatgaagcc tacgcgccca gcgtgttgaa atacgaagcg
    24361 gaaggcatca acgcctcaag ccattggttc ctgttcggtg cggggctttc gctttggtcc
    24421 acatggcagg tcagcacagc cattgggata ttcctcggca catccatccc cgaatcatgg
    24481 tcgctggatt ttgcattgcc gttgaccttt atcgcgatgg ttgtccccgc cttgaaaaac
    24541 cgcccgatga tcgcggccgc actatctgct ggcggagtgg cattgctggc gcacaacctg
    24601 ccgttcaaac ttggcctcat cctcgccgcc ctgacaggca ttgtcattgg caccctgttt
    24661 gaaggcaggc aggacgaaaa gggggcgcaa tgaatatctg gctggtcatg gtcatcggtg
    24721 gtttgatcac atttggaatg agattttccc tgatctatct gtttggcagg tttgaaattc
    24781 cagaaacaat gcgcagggct ttacattatg tcccgcctgc tgttctttct gcgatcattt
    24841 tccctgagtt gttatatcac gcaaatcaaa ttgatttttc attcggcaac atacgcctgc
    24901 ttgcaggcat tgttgcgact ctggtagcct ggttcacaaa gaacactctg ctcaccatcc
    24961 tggcagggat gctggccctg ttcctttttc agttgctgta actattttag ggaaataaaa
    25021 agccccgcgg ttgcttttct tcagcaaccg cggggctttt tcaattcact tcatagcacc
    25081 tacatataga tcaaaaacac ctggtcgccg cccgcctctt tggcacgctt catagcctgc
    25141 aatgcctgct ggattaaggg ctccacctcc accgaggtgc tgatacgcgc caccgaagca
    25201 attccagcgc tgaccttgat attcagcggg gtgttgttat gcaccaccac ccgtgaacta
    25261 cgcacccccg tgatgattct ttcggtcacc ttttccgcat ccgcaccgat cacgccggag
    25321 agcatcaaca caaactcatc acccgcccag cggccaatgc aatcataagg tctgcttttt
    25381 tcgcggatgg cttgcgccac aacatgcaaa acctggtcgc caatatccct gcccagtgaa
    25441 tcgttgatca ccttgaaatg atccacatca agggcaatta aactcacagg cattgaagtg
    25501 cgccgtgccc gttccaattc acccatggcc tgcttgaaaa aagccgcccg attcaaaaaa
    25561 cccgtcaggc tatcgaaaac cgcctgatgc tccaactgtt cacgcgccac agccagattg
    25621 ctcgcaagcg agatgatacg ctcagccatg ccaacgcgat tcttcaggtc tttcgccctg
    25681 aacggccgct ggagaatatc atctgcaccg gatggggttt ggtcatcatc ggtgatcttc
    25741 gccatggtca gcaaaatgta caaaggctgg gtcagtttga tcgcccgcgc gcgcaaaata
    25801 aactgcgaac gccgcacgtc gcttgtatcc cagtttgcaa tgacaaaccg cgcctcaccc
    25861 gattgaatat aaggccaggc ttgctcgctg gaagcgatcg gaatcaggct ggtcttgccg
    25921 tcattaaatg cctgttgaat gatcgcaaat tcgttggggt cgttttcaag cacgagtatt
    25981 ttcatggaga taccttccgt gattttatga ttatagcagg ggagtcgaat gggcaaatcc
    26041 ccacatggtc acctgttctg gatttaatat gattttcccg gcttcacgtt gacaaatggc
    26101 atttcccata gtaatatttg ccccgctcaa ccccaaacgc cgaggtagct cagtggtaga
    26161 gcaggggact cataagccct tggtcgggag tccaaatctc cccctcggca ctcgaaaacc
    26221 cgtgcaaacg ggtttttttc tttatcatgc agattcaacc cgatacactc atccgcagca
    26281 ggcgcagaac gatcgctttg atcatcgagc gtgacggcac actcaccgtc cgtgcgccga
    26341 tgcgtgccag caaggcggtc attgaggaat tcattcgcga aaaagcggat tggatcaccc
    26401 gcacacgcaa aaagctgact cccatcctaa ccgccccgga gaagcaattt gtcgacgggg
    26461 aaaacttcct cttcctcggt tcctcctttg acttgaaact ggtccagcga cagcggccag
    26521 ccctgcgctt tgagaacgga ttcacgctca gcatcgccgc gcaaaaaaga ggcaggcagg
    26581 tcttcaccaa ctggtacaag gaacgtgcgc ttgaagtcat cacggaacgc gtcaatcatt
    26641 tttccgcaag atatgggttt caacccaaac aggtgaaaat ctcttctgca aaaacacgct
    26701 ggggatcgtg cagcgccaac gggacgttga atttcacatg gaggctggtc ctctctccgt
    26761 taagtgtgat cgattatgtc gtcgtgcatg agcttgccca tttgcgggtc cgaaatcatt
    26821 cgcgcaaatt ctggcaggtc gtcgaatcca ttgaccctga ttacaaaacc aaaagaaaat
    26881 ggttgcgcga atacggagaa acgttatact tctaacatgg aagaagctga acgcctgtac
    26941 gccgattttc aggtcaagat cgcgggggat gaagtttggc ggcccaatga cctggaattc
    27001 cttcatgcct ccatcacgat cctttcaaac gcaatgggtg gcagtgaaaa tttcaaacag
    27061 cagcttgggg aagttctgat tgaacggacc gacacaggca aacatctcgg gctggcccac
    27121 cgtggacgaa tccaattaag taagagcact cccttctcag cctggtctgt aattcatgaa
    27181 ctctcccatg cctgggatgc aaaacatcac tggatgcttt ccgtgctgct gcaaaaatac
    27241 acaggcggat ttacctcccg ctttttatcg cgcatcaaaa aatggttccc ccggcaatgg
    27301 gatgcaggca gacatggcgc tgagcaaacc ccgggccgct atggaaggaa gcccggcgtg
    27361 aacgcttttg gatattttta tggcgatcgt cccagcggct cgaactggcg cttcaaccat
    27421 aaagaggatt ttgccgaatc gttggtgatg tactgtggct gggggaaaaa caacctgctt
    27481 tcgcgcaccg cccacggacg gatcgaacgc tataccctgc ccaacggtga acgggatccg
    27541 ctctatggca ttgtggatca atggtcagat tatgcccgtt atttctaccc gccagaaggc
    27601 gactacgccc aaaccaaacg ctggaaattt tttgatgagt taattcacgg aaaaatccca
    27661 acttaatgaa tagatctctt gcaaaagatt aaagtcttaa ccacaaagat catggaa
//
LOCUS       DAOQJC010000051        27334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_774_length_27334_cov_10.978848, whole genome shotgun sequence.
ACCESSION   DAOQJC010000051 DAOQJC010000000
VERSION     DAOQJC010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 27334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27334
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_774_length_27334_cov_10.978848"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PLE39_09575"
     CDS             complement(<1..489)
                     /locus_tag="PLE39_09575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE68618.1"
                     /translation="MRLKNRTAIITGATSGIGKATAFRFADEGADLVITGRRSGLGKT
                     VEAECRRRGVRCVFVEADHTKQEDCQRVVDVAMKEFGRVDILFNNAGIVPKGTAETTT
                     EEVWQKTLDINITAAWRMSRLVIPHMRAQGKGAIVNNGSDWSVVAGRNVVAYTMSKGA
                     VAM"
     gene            549..2963
                     /locus_tag="PLE39_09580"
     CDS             549..2963
                     /locus_tag="PLE39_09580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNE68619.1"
                     /translation="MDSKTLNVLEYPKILERLAGFCDFSASMDLARTLEPTSSFELAS
                     ARLAETTEARKLLSINSMGVGAAHDIRPQADLAARNGVLDPQALLDVKSTLISCRELK
                     KAFEKRTEEYPRLTQIAMGLPETHGLVDAISRILSDRGEILDSASPKLGDIRRSLRVA
                     HDRLMSRLQKYVTDSKTVSMLQEPIVTQRDGRYVIPLRAEFKGKIKSIIHDQSSSGAT
                     LFIEPLPVVEANNEIRELQLAERDEERRILAEVSRQVGEHATELKYGVENLAVLDLAF
                     AKAKYADELRASEPLMRELKVAGSKSSTFQPSTLKLIEARHPLLDPNTVVPVDVDPAP
                     GTRAIVITGPNTGGKTVSLKTVGLLVVMAQSGLHIPAQSGSELPCFHSVWADIGDEQS
                     IEQSLSTFSGHITNIIRILKKIDSRSLVIFDELGSGTDPQEGAAIARAILTHLLESGA
                     MTLVATHYPELKSFAHSTEGVVNASLEFDIQTLRPTYKLTLGLPGRSNALLIAQKLGL
                     PQTIIESARAEINPLDLRADKLLDDIRKERNRTSREREKLEKARAKLETQTRELEKRL
                     EKIEDERRETLAKARAEGELEVAVLKRNIDSLKGQLKKASQPLQAIKAIEEKMEKMEE
                     KVEAPVERRNEQLSVTGNQAWKLGERVTVSTLNAEGVVTALGESDAEIQIGTLRVRAR
                     LSDLLKGGGQSSSTASKPSPVSIKQKADGGTRTSVAVAKSPGMEVDLRGLMADDALDK
                     MEKYLEQAFLSGLPFVRIIHGKGTGKLRQAVREALRGHEYVSSFEEGGDKEGGEGVTV
                     AKLKSG"
     gene            3040..3282
                     /locus_tag="PLE39_09585"
     CDS             3040..3282
                     /locus_tag="PLE39_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68620.1"
                     /translation="MSIDDAIKKMTDLIKATCASAEIKSAKMSDEEARLSVYAPAGDM
                     QKIKDATFQPAIDLLNTDGMDVQVFVYDKDAPPLKG"
     gene            complement(3287..3799)
                     /locus_tag="PLE39_09590"
     CDS             complement(3287..3799)
                     /locus_tag="PLE39_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE68621.1"
                     /translation="MNILAEIHRHENLNIHGRAVCRIAIRGIALHGRKLLMVHSTEVG
                     DYKFPGGGLNAGETHEQALIREVQEECGMSLTCFGAEVGVVVEYLRPQERDCDVFQMT
                     SHYYQCEVDNVFGAQKLDAYEKDLGFKPVWLDVDETIRANKLLLARKSKPGWLKREIL
                     VLEYIRQNLI"
     gene            complement(3897..5021)
                     /locus_tag="PLE39_09595"
     CDS             complement(3897..5021)
                     /locus_tag="PLE39_09595"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNE68622.1"
                     /translation="MNERVKELDGVRGIAIFVVVAFHLLKRADYFTNNEILHFISRLS
                     SIGWVGVDIFFVLSGFLITSILLKTKAEKNYFKNFYIRRALRIFPLYFVFIAIILILI
                     PKLDPDFVPEVPRALPFLLLYQQNWLMLFSKIPLTTYLAVTWSLAIEEQFYLIWPAVV
                     YFTKKETLVKLSIGVILCSILARVLGILFWDDMSVISTFFYYNTFTRFEQLVLGGLLA
                     VAFTEPFWVQCLKNISVSTFWVAFAAFLALSFTAYPHMVPLDENIWLVLGGYTLVAVF
                     SAALVTILMTHPETSIFRRVFQNKGLIFFGKYSYSIYLLHVPVILILLDVLWRTQLRG
                     WSMYLAYIVLTYAITIVGALLTWNLLEKHMLDLKKYFEYQ"
     gene            complement(5145..7154)
                     /locus_tag="PLE39_09600"
     CDS             complement(5145..7154)
                     /locus_tag="PLE39_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68623.1"
                     /translation="MMHGPGRAGEFLKNETIKPRNVSETLSRLGGYFGRFWFMLTLAV
                     IFVITATWTQVTSPELIGQATDCFLVPNGSNPFASFAPTEDSDAQTESSCWLASDPAT
                     LTGRRLLLNQLYATGGYTLPDITTATIEQRTEGLVRLIVFIIILFVAGSLLNGMTFFT
                     MSWTGQHVLRQMRVEVFEQLHRLSLSYYAEHEAGDLMSRITNDTTAIEQAFSFALVNV
                     FSGILLLVWVSYNMITANLPFALLSLVVAPLMFIATFYFSEQARKAFRKSREEIGNVN
                     AELQESIAAVREAQAFNRADENIENFKEVNAANRDANVRAVAYTSALAPTLEALSYVS
                     LAIVTGVGGWYLLKGGTFLGSAVTLGLVVTFLAYVQRFNQPIQQIAVLWTNIQNAIAG
                     AERIFTILDEKPAVTDKPNAKEMKEIKGLVEFDKVSHEYEDGVPVLKGVSFTAQPGQT
                     FAIVGPTGAGKTTIINLLPRFYDVTEGSVKIDGVDVRDVAAESIRRQVGIVLQDTFLF
                     SASVMDNVRFGRPDATDEEVMAAIKLANADSFIERLPEKYNTVLGERGSGLSQGQRQL
                     LSIARAALADPRILILDEATSSVDTRTERLIQKAFDKLLSGRTSFVIAHRLSTIRNAD
                     QILVLKAGQIIERGRHDELLEKKGFYYDLYMSQFKKQEEMEAAMA"
     gene            complement(7151..9106)
                     /locus_tag="PLE39_09605"
     CDS             complement(7151..9106)
                     /locus_tag="PLE39_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68624.1"
                     /translation="MQQTHVRRIVQKRPERTKSKGGMRRAIKYLTNYKRQAALPYLFL
                     IVATLSQLAVPNMIRRVIDAVTSGYIADQILGALDKIPAQFIGTALPQILDVMKYPAE
                     WTADQLKVQLEADVTNAPQLLTSAIVAILVFAILRGVFAFLQAYWAEKNSQAVAYDLR
                     NDLYSKIQTLSFSYHDKNQTGQLMIRATDDVEKVRLFIGQGLLQLIGAFILLAGTVII
                     LFSTNVSLAWAVMPILPVALVLFIIFAGASRPMFAQVQQKLSALNTVLQENLAGIKVI
                     KAFTRETQQQDKFKARADDTMEQAIKLSRLFTFMFPLVFMIANLGQAAILYVGGKAIM
                     AGTLSLGEWQEFSMYLMYLFMPIMMMGMIITQFGQASASADRIFEILDAKNDIADKPG
                     AVKIPAVKGTVKFEDVTFRYFGGGDPVLSNVTFEAKPGETIALLGATGSGKTTIINLL
                     PRFYDPTEGRITIDGYELRDVTLESLRSQIGIVLQETTLFSGSIRDNIAFGKPDASQE
                     EIEAAAKAAQAHDFIMSFPEGYNTHVGERGTTLSGGQKQRVAIARALLLNPRILILDD
                     STSSVDLNTEAAIQSALDVLMKGRTSFVIAQRISTVMNADKIIVLEKGRVVAIGKHAD
                     LLEDDPIYAEIYNSQILANEHEKEGAL"
     gene            complement(9201..9650)
                     /locus_tag="PLE39_09610"
     CDS             complement(9201..9650)
                     /locus_tag="PLE39_09610"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE68625.1"
                     /translation="MSKTLPITHPLRTWMDVFMHRSMRGWAHFAKSTGLSMPQFSILM
                     QLHHKGPCGMSTISERFDITAAAASQLVEKLVQGGYLERAEDPNDRRAKLLRLSKEGA
                     KLVSQGMEERYRWMDELVSALNEDEQTQVTEALEILTRAAQALEDKK"
     gene            complement(9831..10175)
                     /locus_tag="PLE39_09615"
     CDS             complement(9831..10175)
                     /locus_tag="PLE39_09615"
                     /inference="COORDINATES: protein motif:HMM:NF025660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4377 domain-containing protein"
                     /protein_id="HNE68626.1"
                     /translation="MKKMILIFTLAICFTVFFSACGDGTQVNTREMTLFVGPHQVDCT
                     GGPQGKCFQVKYSAEDSEWVNFGNVIQGFEWEEGYEYELSVKFIETRPKNYDYIIPSF
                     ELIEVLEKTKIE"
     gene            complement(10184..11392)
                     /locus_tag="PLE39_09620"
     CDS             complement(10184..11392)
                     /locus_tag="PLE39_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE68627.1"
                     /translation="MLEKFFSLRCPDCGGKITEEERYCPHCGVDLEAPISPSIGSNED
                     EHSPARQHFKAAVKGYEMTTKAGLRDALVNCDLALQYDPNFADAHNLRGLVLDEMGRL
                     EEAITAYREALRLNPDLGDARINLEDAVAERANNQSEFGFTNEAIGFAEEEKPRNRGF
                     VKRIPSILALLMIFFVSFLKSDLIKNLANAYLLPKTSVVFIPDVPGGIAVSHADLEEA
                     AQVLSERSQRLGYSNITFEVSTAGEIVGMIPVTMDAVEFSEQVGDVGLLEFVDFGEKP
                     ALAGDIIRTDLESKYLQQVDGHEWHTVMSNEGVLSAASYRSQTDFGFQVSFSLNAEGA
                     QIFLKHTSENVGTYLGIVLDKRVVSVPMINQPITDGQGMISGTFTEEEAKELAVILQT
                     KPLPFPVRLR"
     gene            11592..12989
                     /locus_tag="PLE39_09625"
     CDS             11592..12989
                     /locus_tag="PLE39_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68628.1"
                     /translation="MSLSEEPIIPTEPTPPSPDPSSLPPRPRRRRATRRDMIPTDAEG
                     QAALISELSRRAYPSIELFVFSLVAGAILGLGFLRDSQAILLLGILITPLMIPWVGFL
                     LASLTGSPRFLFETLMALLISGVIVFIGGLLSGFAARLFLPITLTNVFIHARLWPEEL
                     FVLAIGAITLVASFVRSESKPFLPSVIVAYTFYLPINASGFGLGSGVDGIWPEGILVF
                     ATHFMLASILGLITLLVLRLRPSNRGLILSGVMLLVFTGTLFSLMGNGFPLTTPQTVS
                     TSTPTPSTQPTALPSLTPSLPPTTTNTVRPSNTPKPTNTPSVDETLSATPTEETENTA
                     TNTSTATKPAATPTKTTSTPAATSTNEPIPTVTMTIEVQTIPGTISADEGGGANLRQT
                     PNGKYLMTLDNNTVVEIYPDFKVVNGITWLHVYVIRNDQQIEGWLLESVVSYATPEPN
                     FEPSSTPEIGITPAP"
     gene            13088..14548
                     /locus_tag="PLE39_09630"
     CDS             13088..14548
                     /locus_tag="PLE39_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNE68629.1"
                     /translation="MPIHFGTDGWRAVISDSFTFDNLRIVTQAIADAVASDHWDKSVN
                     GENGPDKKTIVVGYDTRFLSDRFAGEVARVLAANGFTVLLAQSDSPTPAISFAVKHQR
                     AIAGIMITASHNAPRYNGLKLKGAFGGSALTEQCRRVEVYINDNEEQARGPNLMDFNK
                     ARDAGLIQKFNPLPAYFEHLQKLIRTDIIADNPQRFVVDAMYGSGRGVIKSFLQGTGC
                     EIAEIRGEMNPGFGGVHPEPIAKYLGPLASAVSSGMGNFGVVTDGDADRIGAMDERGN
                     FVDPHKIMALSLKYLVEKRGWSGAVVRTVSTTRMIDRLAKRYGLKLYETPVGFNHIAD
                     YMMKEDVLIGGEESGGISFKGHIPEGDGPIMGLLLVEMIAEAKKSLVEMVDDLLTDVG
                     PALYERVDLRLSRPVAKAEMTEFLTQKAPKEIGGQKVDEISQRDGVKYIMADDSWLLI
                     RPSGTEPVLRVYAEGRNADMVKALMGYGKTVAESVV"
     gene            complement(14668..18393)
                     /locus_tag="PLE39_09635"
     CDS             complement(14668..18393)
                     /locus_tag="PLE39_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE68630.1"
                     /translation="MKDDNLIPARKIAGIFRDTSRLRRIFYFFIGITSLVLVGGILVV
                     LSTNDVTAALIIGGNLFLMPISTYLVYREKFDVAAGVLAVMLILSNTLLATWGLGIHS
                     INIIAFSTILVMGSLVTRKWMLFSLVILTVLCIGWLVFGELWGLYTPGVLVHSVAGDF
                     FSASILVLTTAILTYKLTEFLFDGFSLLQQEIAERKSVEYDLRERDAIFEAVTFSAEQ
                     FLKSSDWRDSINLVLERLGRTIHASHAYLFEHHNGPDNEMVDSMRYEWTAPGYASDLN
                     NLEFQNSPLNDEGFEDYHQILMNGQPFVGNTSTFQPAEREYFSSLGIKSILEVPLFVN
                     GQWWGTIGFDDYEVERTWNFSELDALKIAGGILSAAIQRQKADSALQESERIYRQAIE
                     AAGAVPYYQDYRQNRYTFIGNGIQRIVGYSPQDFSPDTWNKIVMEAIPLGEGRGQRIE
                     DVVQQVRSGKIRAWQSDVRVTLPNGEERWVNDSAIEMFDDSEVSYASIGILQDVTERK
                     LAEFNLKKHESLLEAVTFAAEQFLKTANWRETIDVVLERLGREFNSSHAYLFQKHAAP
                     NDQVVSSMIHEWTAPGLETDLDTPEFQNMPKNPMGFDRLYKILDSGEPLVGSASFFTT
                     EEKAYLHSMNVAALLEIRIIVNGTHWGTLGVDDIYHEREWTTMEVDVIKVAASVLGAA
                     IERQLTDDALKHELAERRRAEFALRFSEEKFSKAFHTTPVLMTIENSEGRFIDANKSF
                     MDTLGLKREHVIGQSASELGVFQSPQDVHKLRDTFQEKGAIKDLEIGFRRKNGETGVA
                     LLSSERFYVDAGMYTLTSALDITDRKRAEQERERLIADLRLKNAEAETIRETTTIVIS
                     TLDISETVQRILEQLKRVVSYDSASVWLYKGRVAQLMGGIGLPELTEQDKSYIVNETE
                     PDYIFWTQELPYILIGDIQKDYPQFPDFLHGWLSVPLKLRGRLLGFIALDSLTANQFT
                     EADAQMALTYANQVSIALENARLFSDVQNQLNERQKLVNELENKNSELERFTYTVSHD
                     LKSPLVTISGYLGYLEKDALAGKMDRLKIDLQRIQEAVRKMQTLLNELLELSRIGRMV
                     NASQIIPFDELIREAVDIAHGQIQESGATLQTHPNLPAVYGDKPRLVEVMQNLIDNAS
                     KYMGSQPNPQIEIGFRDRQENLLTFFVRDNGIGIEPIHHERIFGLFEKLDAKSEGTGV
                     GLALVRRIVEVHGGKVWVESSPGQGATFYFTLPAADS"
     gene            complement(18454..>21824)
                     /locus_tag="PLE39_09640"
     CDS             complement(18454..>21824)
                     /locus_tag="PLE39_09640"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE68631.1"
                     /translation="VRIFYWFTALILLSLLSGGIISFLTQSFLAVALLLASVPFVLIP
                     LWFIRQQKVELAASLLAVVLLSLLTVLATYGLGIHQISNLGFPAVLIIASLVTRKRTM
                     VFLTAFAIGCAAWLVFGELYGVYTPVPFVQSLPGDFVSVAVALLITAIMVRFLTEAFF
                     KNNLLLQQELKERKRAEEKYRNIFENTIDGIFQSTVDGRFVNVNRSMARMYGYESPEE
                     MLRMVQDISKQIYVSPEQRDVLVQKLSTEYAVVGFEAEECRKDGSTFWVSANIRTVFD
                     EAGNILYYEGTVEDISLRKMVEMERKQAEDRLLQFRKLMDEANDSIFLIDPQTSRYID
                     FNRTACEKLGYSREELLQLDVIHVASHVTDLQVWNERVELVRESGGLIFEASYLRKDG
                     TRFPVEVSARMLDVGDNMVIVANVRDITERKLVEEALRESEERFRSVFEGATIGLYRI
                     SLKGNILLANTMLARMLGYESVAELLEQDQAGEPIITDYGRKEFLQAIQRDGEMRDFE
                     TLWMRSDGTFFSVRENAKLIRDKNGGPLYFEGTVEDITEKKLAEMTLRQNQQRLQAFF
                     NQSLDGFFFCILEKPLPWADAADKEEILLYIFNTQRYTDVNNAMLEQYQITRHHFLSL
                     TSRDIFTHDLQQGLNLRRKLFDEGHLHLETYERKTDGTPVWFEGDYVCMYDERKWITG
                     FFGIQRDITDRKKVEAERETLIQKLAAKNAESETLRESLAGIVGMFEFLEIVENILDQ
                     IRRVIPYDSASVWKWEDNRQKFICGRNLPVTFQQNEFELDINEDNSALPILLGKVPYV
                     LNNNVQEELKDFRHEPHTYVNSWLAIPLKTRGKIMGLIALDGRSKDQFNEHHTELAVT
                     FANQVAIALENASLFSELQNELGERGKLIKELELKNAELERFTYTVSHDLKSPLVTIN
                     GFLGYLKGDTASGNLDRVRSDIQRIQDAVDKMHLLLRDLLELSRIGRIMNPPETINIE
                     ALVNDAREIVHGRLEAQNVTIEIIPPVGTHPGLPAVYGDRQRLTEVLQNLIDNAAKYM
                     GNQPSPRIEIGHQGVENGQLVFFVKDNGMGIDPEHHERIFGLFNKLDAKSEGTGVGLA
                     LVKRIIEVHGGRIWVESQAGKGATFFFTLPASPS"
     assembly_gap    21825..21924
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21925..24341
                     /locus_tag="PLE39_09645"
     CDS             <21925..24341
                     /locus_tag="PLE39_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68632.1"
                     /translation="RPMQVERYDAGDHVTYEINLITPDATHKNVKVSLFIERFVGGGF
                     AGQVYKTRILKVDGEESPLQVGQTYALKIFIPPSHHALFFRNLLYAIGFQGPFQIQCN
                     PVAARAGALWQKFIRRAASLRFQDTDAVNDVHGTFIDYDLGSCGEISNWVEGRTWRLE
                     VNDHADLLNRWENGKLAEGTSIGSLEYRTKKTFMHEFSALLHEMGAHEFARQYEWSTW
                     KSQPNVLKRLNTDLEPARGLTAVDFRAGLTLLPFLPMSPGDIKLILQGIKRGSLVQFD
                     RGDVNQLESFIKKNSADFSDLLPLLEELKTCELVYRNSIPDITHHHLNLIRDKALRAT
                     IVNSTITGWSVKKIVDTDAEKRLHANKSFFLFFILLGFIPFLGKILRLAFGRQDYRDH
                     YKKMLSSFTYFQRAMFANRMEALIRWYRGERITETLAERISSSLLLYLYHIPLSILPK
                     KIHRLLSDRGFFMDTLYNTIIRPVRLYFNPVLREEWLREIVSESRDRQVLSKEDAEEI
                     LGQIHDPYIHKYLQSLAVHIAMAPITHVISTGLAILYLLNHPEIPRAEAYATAAGILA
                     FFQVIPVSPGSFARGLYVLFLAIKERNLKDYNLALPLSFFKYVGYISFPIQMTYSYPT
                     LARCMAGFWATRMARVIPVFGEGGALLEYKTFNIFYNWPLTIRRKMNERAEWRKTQKT
                     RSWHIIPIVALISAASWLLQKLHISTTGMLPPFALVAPSLILFGLLGGILVNTGSGGS
                     SFSRRVLMAVGSGVSIGFLAALGLLILSPEVETGLIAWISTIIWYSFITATFSTTSAV
                     ITEFKV"
     gene            complement(24446..25027)
                     /locus_tag="PLE39_09650"
     CDS             complement(24446..25027)
                     /locus_tag="PLE39_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68633.1"
                     /translation="MSANALKRTLVFVPILFALACSTLTPKPALSASEIEKEEEAIYA
                     FFAGTSSAVILENTSAGIGNPTPQEMRRQLQDTFQNISGDTIESFVERNAEPGQLSAE
                     MELGVDYVLLSEKELAEISSQPDWGETLAERYPGSYGYLIFSRVGFNRTLDQAVIYVG
                     NVAGPLMGSGDIFLMEKVNGEWVIKEQFMIWIS"
     gene            complement(25170..25586)
                     /locus_tag="PLE39_09655"
     CDS             complement(25170..25586)
                     /locus_tag="PLE39_09655"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HNE68634.1"
                     /translation="MKRILLTMFVTAFLFSACASAPATSLPTEAPISSTLPALTDPSQ
                     PITVKAGDTFMIVMESNPSTGYHWEMIGPLDDAHLELVSREYTASEPVMPGSGGVEVW
                     TLKAVSAGETVLVLGNYPPAQGESYTTETRFSISIQ"
     gene            complement(25720..27039)
                     /locus_tag="PLE39_09660"
     CDS             complement(25720..27039)
                     /locus_tag="PLE39_09660"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE68635.1"
                     /translation="MPHGASKPGYKLSDEVFQNIFLQAGDGIFLIDAQSCMLEMNPRG
                     CEILGYSREELIGQPVLSFQPEDELPHIQEKLSQLAVDKLVMTESFFIRKDGVRIPVE
                     ITGKLLSNGQIIGMLRDISERKQTEQALINSEQRFRSLVEQSPDGIFMADENGLVVEW
                     NRGQEAISGLPRHEALEKPIWEIHWNLMPDELRTHVMFARLEENFKEILASGLGPGLN
                     LPREGVFQHSDGIYRNVEIMMYTYKTDLGYRIGGIVRDITERKQAERRLEYLAMHDVL
                     TDLPNRQLFQDRLEIALGRAGRKPYGKLAVMLLDLDNFKEVNDLHGHIYGDHLLKVAA
                     LRLQTCLRKSDTAARMGGDEFTLIIEEANDLETCSSIAQKVLETLSQPMELDGKVLKV
                     TASIGISFLSPENDNAATLLRKADIAMYQAKRTRNCYKFYEIDVRAY"
BASE COUNT         6998 a         7213 c         6456 g         6567 t
ORIGIN      
        1 catcgccacg gcacctttgc tcatggtgta ggccactaca ttcctgcctg ccactaccga
       61 ccaatctgaa ccgttgttga cgattgcccc cttcccctgt gcgcgcatat gcggaatgac
      121 aagcctgctc atccgccatg ccgcggtgat gttgatatcg agggtctttt gccagacctc
      181 ttcggtggtt gtctccgctg tacccttcgg cacaatgcca gcattattga acaaaatatc
      241 aactcgacca aattccttca tggcgacatc caccacccgt tgacaatctt cctgtttcgt
      301 atgatctgcc tcaacgaaca cgcatcggac tccccgccgc ctgcattcag cctcgacggt
      361 ttttcccaac ccggagcgtc tgcctgttat gaccagatca gccccctcgt ctgcgaaacg
      421 aaacgcggtg gcttttccaa ttccgctggt ggcgcctgtg atgatcgctg ttctattttt
      481 gagtctcatc ccgttctcca ttcttcacca tgctgtattc atcgtttcaa gaagtataat
      541 tcatcattat ggacagcaag acactcaacg tactcgaata cccaaagatc ctcgaacggc
      601 tggcagggtt ctgcgatttt tccgcttcga tggacctggc acgcacactc gaaccaacct
      661 cttccttcga actcgcctct gcccgtctgg cagagaccac cgaagcgcgc aaattacttt
      721 ccatcaacag catgggtgtg ggggctgcgc atgatatccg cccgcaagcg gacctggctg
      781 cacgcaacgg cgttcttgac ccgcaagccc tgctggatgt caaatccacg ctgatctcgt
      841 gccgcgaatt aaaaaaggcg ttcgaaaaac ggacagagga atacccgcgc ctgactcaga
      901 tcgccatggg gctgcctgaa acccatggcc tcgtggatgc gatttcacgc atcctttccg
      961 accgcggcga aattctggat tcagcctcgc caaaacttgg cgacattcgc cgcagtttgc
     1021 gtgtggcaca tgatcgctta atgagccgac tgcaaaaata tgtgaccgat tcgaaaaccg
     1081 tttcgatgct gcaggagccg atcgtcaccc agcgcgatgg gcggtacgtg attccattgc
     1141 gggcggagtt caagggcaag atcaaatcca tcatccatga ccagtcttcg agtggagcca
     1201 cgcttttcat tgaaccgctg ccggtggtgg aggccaataa tgagatccgc gaactgcaac
     1261 tggccgagag agatgaagaa cgcagaatcc ttgccgaggt ttccaggcag gtcggtgaac
     1321 acgccacaga gttgaagtat ggtgtggaga acctcgccgt gcttgacctt gcgtttgcaa
     1381 aagcaaaata cgccgatgaa ctccgcgcaa gtgaaccctt gatgcgtgag ttgaaggttg
     1441 ctggttcaaa atcttcaact tttcaacctt ccactttaaa gcttattgaa gcccgtcacc
     1501 cattgcttga tccaaatacc gtcgtccctg tggatgtgga ccctgccccc ggcacacgag
     1561 ccattgtcat cacggggccc aacacaggcg gcaaaacggt ctcgttgaaa acggttgggc
     1621 ttttggtggt gatggcgcaa agcgggctgc acatccccgc acaaagcgga tctgaactac
     1681 cgtgcttcca ttcggtgtgg gctgatattg gcgatgagca atccattgaa cagtcactct
     1741 ccacgttcag cggacacatc accaacatta tccgtatcct aaaaaaaata gacagccgtt
     1801 cactcgtcat tttcgatgaa cttggctcag gcaccgaccc gcaggaaggc gcagccattg
     1861 cgcgtgcgat cctaacccac ctgctggaat cgggtgcaat gaccctcgtt gcaacacatt
     1921 accccgagtt gaaatccttt gcgcattcga ccgaaggagt ggtgaacgct tctctggaat
     1981 ttgacattca aacgcttcgc cccacctaca aattgactct cggtctcccg ggcaggtcca
     2041 acgccttgtt gatcgctcaa aagctgggat tgccgcaaac catcatcgaa tcagcacggg
     2101 ctgagatcaa tccgctggat ctgcgggccg ataaactgct ggatgatatt cgcaaggaac
     2161 gtaaccgcac ttcacgcgag cgcgagaagc tcgaaaaggc acgcgccaaa ttggaaaccc
     2221 aaacacgtga attggaaaaa cgccttgaga agatcgaaga cgaacgccgc gaaacactcg
     2281 ccaaagcccg cgcggaaggc gagttggaag tggcggtgct caagcgcaac attgactcgt
     2341 tgaagggtca attgaaaaaa gccagccagc ccctgcaggc catcaaagcc attgaagaaa
     2401 aaatggaaaa gatggaagaa aaggtggaag cgcctgtgga gcgaaggaat gagcagttat
     2461 ccgtcacagg taatcaggca tggaagttgg gtgaacgggt cactgtcagc actttgaatg
     2521 ccgaaggtgt tgtaaccgct ttgggtgagt cggatgcgga gatccagatc gggactctgc
     2581 gcgtgcgggc gaggctatct gacctgctga aaggcggcgg gcagtcatca tcaacagcca
     2641 gcaagccatc ccctgtgagc atcaagcaga aggctgacgg cggcacgagg acttcggtgg
     2701 cggttgcaaa atcgccgggc atggaagttg acctgcgcgg attgatggcc gatgatgcac
     2761 tcgataaaat ggaaaaatat ctggaacagg cttttctttc agggctgcct tttgtgcgca
     2821 tcattcacgg aaaagggacg ggcaaactgc gtcaggctgt acgcgaggcc ctgcgcgggc
     2881 acgagtatgt aagctcgttc gaagaaggcg gcgataagga aggcggcgaa ggcgtgaccg
     2941 ttgcaaagtt gaaaagcggg taggaaattg gtcaggtctg cggtgtaacc tgctgcagga
     3001 tggtatataa tttttttaaa tttaaacagg gagctttcca tgtccattga tgatgccata
     3061 aaaaaaatga cagacctgat caaggccaca tgcgcaagtg cggagattaa atccgcaaag
     3121 atgtctgatg aagaagcgcg gctttcagtg tatgcgcctg cgggtgatat gcaaaagatc
     3181 aaggatgcga cgtttcaacc agccattgac ctgctcaaca ccgatggaat ggacgtgcag
     3241 gtttttgtgt acgacaagga tgccccgccg ctgaagggtt gatgtttcag atcaggtttt
     3301 gcctgatata ttcgagaaca agaatttccc ttttcaacca tccgggttta cttttacgag
     3361 ccagcaataa tttgttggct cgtattgttt catccacatc aagccacacg ggtttgaaac
     3421 ccaggtcttt ttcgtatgca tcgagtttct gggcgccaaa aacgttatcc acttcgcatt
     3481 ggtaatagtg tgaggtcatt tgaaatacgt cgcaatcccg ctcctgtgga cgaagatact
     3541 caaccacaac gccaacctct gcgccaaaac aagtcagtga catgccgcat tcttcctgta
     3601 cttcgcggat cagggcctgt tcatgcgttt cacctgcgtt cagtccgccg ccggggaatt
     3661 tataatcgcc gacctcagtg gagtgaacca tgagcaattt ccgtccatgc aaggctatgc
     3721 cgcggatggc gattcgacac actgccctgc catggatgtt gagattttca tgtcggtgga
     3781 tttcagcaag aatattcatt gaactccctc aatctttcta tggcgattgg tcaaatgcat
     3841 gcagtttgca aagatatttc cctgaaactt tgacatcaaa aagacaacaa tttcgcttat
     3901 tgatattcaa aatacttttt caggtcgagc atgtgttttt caagcaggtt ccatgtgagc
     3961 aaggcgccga cgatagtgat ggcataggtc aaaacgatgt aagcaaggta catgctccag
     4021 ccgcgcaatt gggtgcgcca gaggacatcc aataaaatca ggatgacagg cacatgcaaa
     4081 aggtaaatgg agtaactgta ttttccaaag aaaataagac ctttattctg aaataccctg
     4141 cggaagatgg acgtttcagg gtgggtcatc aaaatggtga ccaatgccgc tgaaaaaacc
     4201 gccaccaatg tatagcctcc caaaaccagc cagatatttt catccagtgg aaccatgtgg
     4261 gggtaggctg taaaactcaa ggccaggaac gccgcaaatg caacccaaaa agttgagaca
     4321 gagatatttt tcaagcattg cacccaaaac ggctcggtaa atgcaactgc aagcaacccg
     4381 cccaaaacca actgctcaaa acgggtgaaa gtattgtaat aaaagaaggt tgaaataaca
     4441 gacatgtcat cccagaacaa aattcccaac acacgagcaa ggatcgaaca gaggatcacc
     4501 ccgatgctta gtttgaccag cgtctccttt ttcgtaaaat aaacgacggc aggccagatc
     4561 aaatagaact gctcttcgat ggccagtgac caggtaaccg ccagataggt ggtcaacggg
     4621 atcttgctga acagcatcag ccagttttgc tgatacagca gcaggaaggg cagggcgcgt
     4681 ggaacttcag gcacaaaatc ggggtcaagt tttggaatca ggatcagaat gatggcaatg
     4741 aaaacaaagt acaggggaaa gatccgcaag gcacggcgga tgtaaaaatt cttgaaataa
     4801 tttttttcag cctttgtctt cagcaaaatg gatgtgatca aaaaccccga aagcacaaaa
     4861 aaaatatcca cgcccaccca gccgatggaa gagagacgtg aaatgaaatg caggatctca
     4921 ttattggtga aataatcggc gcgcttaagc aaatggaacg ccacaacgac aaaaatcgca
     4981 attccccgca cgccatccaa ctccttaacc cgctcattca taaaacgaaa tatccatttt
     5041 ctgtgctggc ttcgagttca gatggggcat ggcgcttctc ctaaaccccc atttaaccac
     5101 aaaaacagca gtgacacggc gctaccgtgt cactgctaca aaagttacgc cattgcagct
     5161 tccatctctt cctgtttttt gaactggctc atgtacaggt cgtagtagaa gcctttcttt
     5221 tcaagcagtt catcatgcct gccgcgctca atgatctgcc cagctttcaa caccaggatc
     5281 tggtctgcat tgcggatggt ggaaagacga tgcgcgatga caaaggaggt gcgtccgctt
     5341 aaaagtttat cgaatgcctt ttggatgaga cgctcggttc tcgtatccac actggaagtt
     5401 gcttcatcca gaatgagaat tcgcgggtct gccagagccg cacgcgcaat ggacaacaac
     5461 tggcgctgtc cctggctcag ccctgaacca cgttctccca aaacagtatt gtatttttcg
     5521 ggcaggcgtt caatgaacga atcagcattg gcaagtttga tcgctgccat cacctcttca
     5581 tcggtggcat cgggtcgtcc aaagcggacg ttatccatga ccgaagcgga gaagaggaac
     5641 gtatcttgaa gcacgatgcc aacctgcctg cgaatggatt cagcagcaac gtcgcgcaca
     5701 tccacgccat caatttttac cgaaccttcg gtcacatcat agaaacgcgg aagcaggttg
     5761 atgatggtgg tcttgcccgc tcctgtgggg ccgacaatgg caaaggtctg cccgggctgg
     5821 gctgtaaatg aaacaccctt caacacgggc acgccatctt catattcatg ggagaccttg
     5881 tcaaactcaa cgaggccttt gatctccttc atttcctttg cgttgggttt atccgtcaca
     5941 gcaggttttt catcgagaat ggtgaagata cgctccgcac cagcgatggc attttgaatg
     6001 ttcgtccaca acacagcgat ctgctggatc ggctggttaa agcgctggac ataagcaagg
     6061 aaggtgacta ccaagccaag ggttacagca gaaccgagga aggttccgcc tttgagcaga
     6121 taccacccgc ccacaccagt gacgattgcc aacgacacat aagacagggc ttccagggtc
     6181 ggggccaggg ccgaggtgta agccacagca cggacgtttg catcgcgatt ggcggcgttg
     6241 acctctttga agttttcaat gttctcatct gcacggttga aagcctgggc ttcccgtacc
     6301 gcagcaatgg attcctgtaa ctccgcgttg acattaccga tctcttcgcg tgattttcta
     6361 aaagccttgc gggcctgctc ggagaaataa aacgtggcga taaacatcaa cggcgcaaca
     6421 accagtgaaa gcagggcaaa cggtaaattg gccgtgatca tgttgtagga aacccacacc
     6481 agcagcagaa tgccgctgaa cacattgacc agcgcgaacg aaaaagcctg ttcaatggcg
     6541 gttgtatcgt tggtaatgcg gctcatcaag tcaccagctt catgctcggc ataatagctg
     6601 agcgaaaggc gatgcagttg ttcgaacacc tccacacgca tctgacgcag cacatgttga
     6661 cccgtccacg acatggtgaa gaaagtcata ccgttcaaaa gggaacccgc cacaaagaga
     6721 atgatgatga acacgatcaa acggaccaat ccttcggtgc gctgttcaat tgtggcagtg
     6781 gtgatatcag gcagggtgta accacccgtg gcgtagagct gattaagaag caggcgtctg
     6841 cctgtaaggg tggcggggtc tgaggcaagc caacaggaag attctgtttg ggcatcagaa
     6901 tcctctgtcg gtgcgaatga agcgaacggg ttgcttccat tcgggacgag gaaacaatcc
     6961 gttgcctgtc cgatcaattc agggctggtt acttgcgtcc aggtggctgt gatgacaaag
     7021 atcacggcca gagtcagcat gaaccagaag cggccaaagt atccgccaag acgtgaaagg
     7081 gtttcgctca cgttgcgggg tttgatggtt tcgtttttaa gaaattcgcc tgcgcggcct
     7141 ggtccgtgca tcataatgca ccttcctttt catgttcatt tgccaagatc tgagagttat
     7201 aaatttctgc gtaaatgggg tcatcttcca gcaggtcggc atgtttgcca attgcaacca
     7261 ccctgccctt ttcgagcacg atgatcttgt cggcattcat cactgtgctg atgcgttgcg
     7321 cgatgacaaa ggaagtgcgt cccttcataa gcacatccag cgcagattgg atcgcagctt
     7381 cggtgttgag gtcaacactt gaagtggaat cgtcgaggat gagaatgcgc gggttgagaa
     7441 gcagggcacg ggcaatggcg acgcgctgct tttgtccgcc agacaaggtg gtaccgcgct
     7501 cgccgacatg tgtgttatag ccttcaggga acgacatgat aaagtcgtgc gcctgtgctg
     7561 cttttgcagc ggcttcgatc tcttcctgtg aagcatcggg tttaccaaag gcgatgttat
     7621 cgcggatgct tccgctgaac agggtggttt cctgcaacac aataccgatc tgcgaacgca
     7681 gtgactcgag ggtcacatcg cgcagttcat aaccatcaat ggtgatgcgg ccttctgtgg
     7741 ggtcatagaa acgcggcagc aggttgatga tggtggtctt gcctgaacct gtggcgccaa
     7801 gcagggcaat ggtctcgccc ggtttggctt caaaagtgac attggaaagc acagggtcgc
     7861 cgccgccaaa ataacggaag gtgacatctt cgaatttcac ggtgcccttc acagcaggga
     7921 tcttgaccgc gccgggctta tcggcaatgt cattctttgc atcgaggatc tcgaagatgc
     7981 ggtcggcgga tgcagaagcc tgaccgaatt gagtgatgat catgcccatc atcatgatcg
     8041 gcataaagag atacatcaaa tacatcgaaa attcctgcca ctcgccaagg ctcaacgtgc
     8101 cagccatgat ggccttgccg cccacataca aaatggcggc ctgccccaga ttggcgatca
     8161 taaacaccag cgggaacata aacgtgaaca agcgtgaaag tttgatggcc tgttccattg
     8221 tgtcatcggc acgcgctttg aacttatcct gctgctgggt ttcacgcgta aaagctttaa
     8281 tgaccttgat acctgccagg ttctcctgca acaccgtgtt caaggcagaa agtttctgct
     8341 gcacctgtgc gaacatcggg cggcttgctc ccgcaaaaat gatgaacaat accaatgcca
     8401 caggcaaaat cggcatcaca gcccatgcca gtgaaacgtt cgtcgaaaac aaaatgatca
     8461 ccgtacccgc caacaggatg aatgcgccga tcaactgaag caagccctgg ccgataaaga
     8521 ggcgcacctt ttccacatca tccgtggcgc ggatcatcaa ttggcctgtc tgattcttgt
     8581 catgatacga gaatgacagg gtctggatct tggaatacag gtcattgcgc agatcgtaag
     8641 ccaccgcctg cgaatttttc tccgcccaat atgcctgcaa aaatgcgaag acgccgcgca
     8701 agatcgcaaa aacgagaatg gcgacaatgg cactggtcag aagttgtggg gcattggtaa
     8761 catccgcttc caactggacc ttgagctggt ctgccgtcca ttcagcagga tacttcatca
     8821 catccagaat ctgcggcagg gctgtgccaa taaattgcgc ggggatctta tccagcgcgc
     8881 caaggatttg gtctgcaatg taaccgctgg tcaccgcatc aatgacacgt cggatcatat
     8941 tcggcactgc caattgtgac agtgtggcta cgatcaggaa taaatatggc aacgctgcct
     9001 gacgcttgta attcgtcaga tatttaattg cgcgcctcat gccgcctttg gacttggtcc
     9061 gctcggggcg tttttgaacg attcttctta catgagtttg ttgcatgttg ctcctattaa
     9121 taattggaga ttggtaattg ggactatgaa tatcaaaaaa atgtgcagaa actatggaaa
     9181 agaaggccag aaattgcgga tcatttctta tcctccaaag cctgtgcggc acgggtcaaa
     9241 atctccaacg cttcggtgac ctgggtctgc tcatcttcgt tcaaagcaga gaccaattcg
     9301 tccatccacc gatagcgctc ttccataccc tggctgacca gttttgcgcc ctcttttgag
     9361 agacgcagca attttgcacg acggtcattt ggatcttcag cccgttcaag gtagcccccc
     9421 tgaaccagtt tttccacaag ctggctggcg gccgcggcgg tgatatcgaa ccgttcgctg
     9481 atcgtcgaca tcccacaagg ccctttgtgg tgcaattgca tcagaatgct gaattgcggc
     9541 atcgaaagcc cggtggattt tgcaaaatga gcccatccgc gcatggaacg atgcatgaat
     9601 acatccatcc atgtgcgcaa ggggtgggta attgggaggg ttttggacat agttaagcct
     9661 tctttttagt taagcggact aaactatatc cccgtatgaa ccttgggtca agggcagaag
     9721 agttagagaa gcgttagaaa atttggaatc gtccaaacaa ggaaaatctc ggcgatatgc
     9781 cctatggaca catcgccgag attttgaaaa agtgcaagag gttgattttt ttactcgatt
     9841 ttcgttttct ccagcacctc gatcaactca aaggacggaa tgatatagtc ataattcttc
     9901 gggcgggttt cgatgaattt gacgctcaat tcatactcat atccctcctc ccattcgaaa
     9961 ccctggatga cattgccaaa attcacccat tcggaatcct cggcggaata cttgacctga
    10021 aagcacttcc cctgtggacc acccgtacaa tccacctggt gcggaccaac gaacagggtc
    10081 atctcacggg tgttgacctg agtaccgtca ccgcaagctg aaaaaaagac agtgaaacaa
    10141 atggccagtg taaaaatcaa gatcatcttt ttcatggata cctttatcga agccgcacag
    10201 ggaaaggcag cggtttcgtc tgtaatatga ccgccaactc tttcgcctct tcttcagtga
    10261 aagttcccga gatcatcccc tgtccatcgg taattggctg gttgatcatc ggaacagaga
    10321 ccacccgttt atccaaaacg attccgagat aggtaccaac attttcggaa gtgtgtttca
    10381 aaaaaatttg cgccccttcc gcgttaagtg aaaaactgac ctgaaaacca aaatcagttt
    10441 gagaacgata cgatgcagcc gacagaacac cctcgttcga catgaccgta tgccattcat
    10501 gcccatccac ctgctgaaga tacttactct ccaaatctgt gcggataata tccccagcca
    10561 atgcaggttt ctcaccgaaa tccacgaact ccaaaaggcc cacatccccc acctgctctg
    10621 aaaactccac agcatccata gtgacaggga tcatccccac gatctcacct gccgtggaaa
    10681 cctcaaatgt gatgttggaa tacccaagcc gctggctgcg ttctgaaaga acctgcgcag
    10741 cttcttcaag atctgcatgg ctcacagcga tgccaccagg cacatcgggg ataaacacga
    10801 ccgaagtctt tggcagcaga taagcattgg ctaaattttt gatcaggtcg ctctttaaaa
    10861 atgaaacaaa aaagatcatc aacaaagcca gaatggaggg aatacgtttt acaaagccgc
    10921 ggtttcgtgg tttctcttct tcagcaaaac caatggcctc attcgtaaat ccaaactcag
    10981 actgattatt agcccgttcc gccactgcat cttcaagatt gatgcgcgca tcaccaagat
    11041 caggattcaa ccgcaatgct tcgcggtagg ccgtaatggc ttcttcgagc cgtcccattt
    11101 catcaaggac cagtccgcgc aaattatgcg catcggcaaa attcgggtca tattgaagcg
    11161 ccagatcaca attgacgagc gcatcacgta aacccgcctt cgtggtcatt tcatacccct
    11221 tcaccgcagc cttaaaatgc tgacgggcgg ggctatgctc atcctcgttt gaacctatgg
    11281 aaggtgagat cggcgcttcc aagtccaccc cgcaatgcgg acaataccgc tcctcctctg
    11341 ttatcttccc gccacaatcg ggacaacgca aactgaaaaa cttttccaac atggcagcca
    11401 tccttgaaat aaatttaccc gccttaactt tatgattctg aaaacatttc gtcaagggat
    11461 tgggctggtt ctttggtacc ttccatataa ttacatgaca aatcaattcc cacctcccac
    11521 tctccattca tcccctgtat aatggaacat catgattctc ccgaccattt cagggtacat
    11581 gtagaaacgc catgtcttta tctgaagaac ccatcatccc caccgaacca accccgccct
    11641 caccagaccc gagcagcctt ccgccgcgcc cgcgccgcag acgcgcaacc cgccgcgata
    11701 tgatccccac cgatgccgag ggacaggccg cgctgatctc cgaattatcg cgccgcgcct
    11761 atccatccat tgaattattt gtgttttcat tggtggctgg ggccattctg ggcctgggtt
    11821 tcctgcgcga ttcgcaggcc atccttttgc ttggtattct cattaccccg ctcatgatcc
    11881 cctgggtggg ctttttgctc gccagcctta cagggtcacc tcgttttctt tttgaaacac
    11941 tgatggccct gctcatcagc ggcgtcattg tgttcattgg cgggctgcta tcgggctttg
    12001 ccgctcgact ctttttaccc atcaccctta ccaacgtttt tatccatgca cgtctttggc
    12061 ccgaagaact ctttgtgctc gccatcggtg ccattacgct tgttgcatcc ttcgtgcgtt
    12121 ctgaaagcaa acccttcctg ccaagcgtca tcgttgcata taccttctat ttgcccatca
    12181 atgcaagcgg cttcggcctc ggctcgggtg tggatggcat ctggcctgaa ggcatcctcg
    12241 tcttcgccac acacttcatg ctcgcaagca ttctcggcct gatcactctg ctggtattac
    12301 ggctgcgccc atcaaaccgc ggcctgatcc tcagcggcgt catgctgctt gttttcaccg
    12361 gcaccctctt cagtttgatg gggaacggtt tcccgctcac aacaccccaa accgttagca
    12421 cctccacccc cacaccttcg acgcaaccca cagcgcttcc ttccctaacc cctagcctgc
    12481 cgcctacaac aacaaacacg gtccgcccct cgaacacgcc caaacctacc aacaccccaa
    12541 gtgtggatga gaccctttcg gccaccccaa cagaagaaac cgaaaataca gccaccaaca
    12601 cctccaccgc tacaaaaccc gccgccaccc ccacaaaaac gacctccact ccggcggcca
    12661 cctcaaccaa cgaaccgatc ccaacggtca caatgaccat tgaagtgcaa accatccccg
    12721 gcacgatcag cgcggatgaa ggcggcggcg ccaacctgcg ccaaaccccc aacggcaaat
    12781 acctgatgac cctcgacaat aacaccgtcg ttgaaatcta ccccgatttc aaagtggtca
    12841 acggcatcac ctggctgcac gtttacgtca tccgcaacga ccagcagatc gaaggctggc
    12901 ttttggaatc agttgtcagt tacgccactc ccgaaccgaa cttcgaacca tcttccacac
    12961 ctgaaatcgg aattacacct gcaccgtaaa aataggatga aatatgaagg atgagggata
    13021 tatcaatcct tccgccttca tatttcatcc tttatctttc atccttcatc cttttggaga
    13081 cccccacatg cccattcact ttggaaccga cggctggcgc gccgtcatct cagatagttt
    13141 caccttcgat aatcttcgca tcgttacaca agccattgca gatgccgtgg catccgacca
    13201 ttgggacaaa tctgtcaatg gtgagaacgg acccgataaa aagaccatcg tggtcggcta
    13261 cgatacgcgt tttctttccg accgttttgc tggagaggtg gcgcgtgtgc tggccgccaa
    13321 cggcttcact gtgttactgg cgcaatctga ttcacccacc ccggcgatct catttgcggt
    13381 gaaacatcaa cgcgccattg caggcatcat gatcaccgcc tcacacaatg ctccgcgcta
    13441 caacggatta aaactcaagg gagcattcgg cggctcagcg ctcaccgagc aatgccgccg
    13501 cgtggaagtg tacatcaacg acaacgaaga acaggcacgc ggccccaacc tgatggattt
    13561 taataaggcg cgcgatgcgg gcttgatcca aaaattcaac ccgttgccag cttatttcga
    13621 acatttacaa aaattgatcc gcaccgacat cattgcggat aacccccaac gctttgtggt
    13681 ggatgcaatg tatggctccg ggcgcggcgt catcaaatcc tttttgcagg gaacaggctg
    13741 tgagatcgcc gaaattcgcg gtgaaatgaa ccccggcttc ggcggcgtac accctgaacc
    13801 gatcgcaaaa tacctcggcc ctctggcaag cgcggtcagt tcagggatgg gcaattttgg
    13861 cgtggtcacc gatggcgatg ccgaccgcat cggcgcgatg gatgaacgcg gcaactttgt
    13921 tgacccgcac aagatcatgg cgctctcttt gaaatacctt gttgaaaagc gcggctggtc
    13981 gggagcagtg gtccgcacag tatccaccac ccgcatgatc gaccgtcttg caaaacgcta
    14041 cggattgaaa ttgtatgaaa cccctgtggg cttcaatcac attgccgact acatgatgaa
    14101 ggaagatgta ctcattggcg gcgaggaatc gggcggcatc tctttcaagg ggcacattcc
    14161 cgaaggtgat ggtccgatca tgggtttgct gctggtggag atgatcgccg aagcaaagaa
    14221 atcgctcgtg gaaatggtgg atgacctgct gacggatgta ggccccgcct tgtacgagcg
    14281 tgtggatttg cgcctcagcc gccccgtagc caaagctgaa atgaccgaat tcctcaccca
    14341 aaaagcgccc aaggagatcg gcggccaaaa agtggatgaa atcagtcagc gtgacggcgt
    14401 gaaatacatc atggccgatg actcatggct gttgattcgc ccttcgggca cggagcctgt
    14461 attacgtgtg tacgcagaag gtcgaaacgc ggatatggtc aaagcgttga tgggatatgg
    14521 aaagacagtt gctgaaagtg tagtgtaaac aaatttagtg tgaaacagaa atggacactg
    14581 accttcgctg caaagagcga aggtcagtgt gtttttaggt aataaaggcc acgatcaaac
    14641 accaaagaaa ttcaagttgc aaccagttca tgaatcggcg gcgggaagtg taaagtaaaa
    14701 cgtggctccc tgccccgggc tgctctccac ccagaccttg ccgccatgca cttcaacgat
    14761 cctgcgtaca agagccaggc caaccccggt gccctcggat ttcgcatcca atttttcaaa
    14821 gaggccaaag atgcgctcgt gatgtatcgg ctctatgcca atgccattat cacggacaaa
    14881 aaaggtcagc agattctcct gcctgtcgcg gaagccgatc tcgatctgcg ggttgggctg
    14941 gctgcccatg tatttggagg cattgtcaat gaggttctgc atgacctcca caaggcgcgg
    15001 tttatctcca tagacagctg gcaggttcgg gtgagtctga agcgtcgctc cactttcctg
    15061 aatctgtccg tgggcgatat caacggcttc gcggatcaat tcatcaaacg gaataatttg
    15121 agatgcgttg accatgcgtc cgatgcggga gagttcgagc aattcgttga gcagggtctg
    15181 catttttcgg accgcttcct gaatccgctg caaatcgatt ttgaggcgat ccattttccc
    15241 agcgagggca tccttttcaa gataaccaag atacccgctg atggtgacca gtggagattt
    15301 caggtcgtgc gagaccgtgt aggtaaaacg ttcgagctcg ctgtttttat tttccaattc
    15361 attgaccagt ttctggcgct catttaactg gttctgcaca tctgaaaaga ggcgtgcatt
    15421 ctcaagcgca atcgagacct gattggcata ggtcagcgcc atctgtgcat ctgcctcagt
    15481 gaactggttt gccgtaaggc tatccaacgc aatgaagcca agcagcctgc cgcgcagttt
    15541 caaaggaaca gaaagccagc catgaagaaa atctgggaac tggggataat ccttttgaat
    15601 atcaccgatc aaaatatacg gaagttcctg cgtccaaaaa atataatctg gctcggtttc
    15661 attgacgata taacttttat cctgctcggt cagttcgggc aggccaatgc cgcccattaa
    15721 ttgagcgacc cgccctttat acaaccagac cgaggcgctg tcgtaactga ccacccgttt
    15781 tagctgttcg agaattcgct gcacagtttc tgaaatatcg agcgtggaaa tcacaatggt
    15841 cgtcgtttcg cggatggttt cggcttcggc atttttcagg cgcaggtctg caatgagtcg
    15901 ttcgcgttcc tgctcggccc gtttgcggtc agtgatatca agtgccgagg tcagggtgta
    15961 catgcccgca tccacataaa aacgttcgct ggaaagcagg gcaacacctg tctctccgtt
    16021 cttacggcgg aaaccgatct ccaggtcctt gatggcaccc ttttcctgaa aagtatcgcg
    16081 caatttatgc acatcctgcg ggctctggaa cacccccagc tcggaggcgc tttgcccgat
    16141 gacatgctcg cgcttcaggc cgagcgtatc catgaaggat ttgttcgcat caataaaacg
    16201 gccttcacta ttttctatgg tcatcaatac cggggtggtg tgaaaagcct tggaaaattt
    16261 ctcttccgaa aaacggagcg cgaattcagc gcgcctgcgt tccgccagtt catgttttaa
    16321 tgcgtcatcg gtcaactggc gctcaatggc agcacccagc acacttgccg ccactttaat
    16381 gacatccact tccatggtgg tccattcacg ttcatgatag atatcgtcca cgcccaatgt
    16441 gccccaatgc gttccattta ctatgatgcg aatctcaagc agggctgcca cattcatgga
    16501 gtgtaaatag gccttttctt cggttgtaaa aaaagatgca ctcccaacca aaggttcacc
    16561 gctatcgagg atcttataaa ggcggtcaaa ccccattggg tttttgggca tattttgaaa
    16621 ctcgggggta tcgaggtcgg tttccaggcc gggggcggtc cattcatgga tcatggatga
    16681 gaccacctga tcgtttggag cagcatgttt ctggaacaaa taggcatgag atgaattaaa
    16741 ttccctgccc agcctctcaa ggaccacatc aatggtctca cgccaattgg cggtttttaa
    16801 aaactgctca gcggcaaaag taacagcctc aagcaaagac tcgtgttttt tcaaattaaa
    16861 ttcagcgagt ttgcgctcgg tcacatcttg aaggatgcca atggatgcat aggacacttc
    16921 tgaatcatcg aacatctcaa tggcactgtc attcacccag cgctcctcgc cattcggcag
    16981 agtcacacga acatccgact gccaggcacg gatcttcccc gaccgcacct gctgaaccac
    17041 atcttcaatg cgctgtccac gcccttcccc aaggggaatg gcctccatca cgatcttatt
    17101 ccaggtatcg ggagaaaaat cctgcggaga atatccgacg atccgctgaa ttccattgcc
    17161 gatgaaggta taacgattct gccgataatc ctgataatac ggaaccgccc cagccgcctc
    17221 aatggcctga cgatagatcc gttctgactc ctgtaaagct gaatcagctt tctgacgctg
    17281 gatcgccgca ctgagaattc cacccgcgat cttgagcgca tccaactcag aaaaattcca
    17341 ggtcctctcc acttcgtaat catcgaaacc gatcgttccc caccactgtc catttacaaa
    17401 aagcggaacc tccaaaatgg atttaatacc caacgaggaa aaatactccc gttcggcagg
    17461 ctgaaaagtg gaggtattcc caacaaaggg ttggccgttc atcaaaatct ggtgataatc
    17521 ttcgaagcct tcatcattca ggggagagtt ctgaaactcc agattgttca gatcgctggc
    17581 atatccgggc gcagtccact catagcgcat ggagtcgacc atttcattat cgggtccgtt
    17641 gtgatgttcg aacaggtagg catgagaagc atggatggtc cgtcccagcc tttccagcac
    17701 gaggttgatg ctgtcacgcc aatcagatga tttcaaaaat tgttcagctg aaaatgtgac
    17761 agcttcaaaa atggcgtcgc gttcgcgcaa gtcatattcg acggatttcc gttccgcaat
    17821 ttcctgttgc agcagggaaa agccatcgaa gaggaattcg gtcagtttat aggtgaggat
    17881 cgccgtcgtc agaacaagga tcgaggcaga aaagaaatct cctgccacgc tatgcaccaa
    17941 caccccgggt gtgtaaagcc cccacaattc accgaaaacc agccagccga tgcacaaaac
    18001 ggtcaagatc accaaggaga acagcatcca ctttctggtg accaggcttc ccatgacaag
    18061 aatggtggaa aaagcaataa tgttgatgct gtggattccc agcccccaag tggcaagcag
    18121 tgtgtttgaa agaatcaaca tcacggcaag cacaccggca gccacatcga acttctcacg
    18181 atataccaga tatgtggaaa tgggcataag gaacaggttg ccgccaatga tgagggcggc
    18241 ggttacatcg ttggtggata gcacaacaag aatccccccc accaaaacaa gagaagttat
    18301 gccgataaaa aaataaaaga ttctgcgtaa acgactggta tctcgaaata ttcctgcgat
    18361 tttccgtgct gggattaagt tgtcatcctt catggcgttc atcctgaata aaatttatgg
    18421 gacatcgttc tgaaagggtg tggtttgaac ctgtcaggaa gggctggcag gcaatgtaaa
    18481 aaagaatgtg gctcccttac ctgcctgtga ttccacccag atgcgccctc catgaacctc
    18541 gataatgcgt ttgacgaggg ccagccccac acctgtccct tccgattttg catcaagctt
    18601 gttgaacagg ccgaaaatac gttcgtggtg ttcagggtct atgcccatgc cgttatcttt
    18661 tacaaagaat accaattggc cattttcaac gccttgatga ccgatctcaa tgcggggtga
    18721 gggttggttg cccatgtatt tcgccgcatt atcgatcagg ttttgcagga cttcggtaag
    18781 gcgctgacga tctccatata cggctggcag gcctgggtga gtcccgaccg gggggatgat
    18841 ctcgatggtc acattttgtg cttcaagcct cccatggacg atctctctgg catcattcac
    18901 caatgcttca atattaatcg tttcaggcgg attcatgatg cggccaatgc gtgaaagttc
    18961 caacagatct ctaagcaaca aatgcatttt atccactgca tcctgaatgc gttgaatatc
    19021 tgatcggaca cggtccaaat tccccgaagc ggtatcacct ttcaggtagc cgaggaatcc
    19081 gttaatggtg acaagcggcg atttcaggtc atgtgatacc gtgtaggtga atctttccaa
    19141 ctccgcgttt ttcaattcca gctctttgat gagctttcca cgttcgccca attcgttctg
    19201 caattcagaa aagaggctgg cattttccag cgcaatggca acctgatttg caaaggtgac
    19261 ggcgagctcg gtatggtgtt cattgaattg atcttttgac ctgccatcca aagcgatcag
    19321 gcccatgatc tttccgcggg ttttcaatgg aatggccagc catgagttga cataggtatg
    19381 tggttcgtgc ctgaagtcct ttaattcctc ctgaacattg ttattcaaca catagggaac
    19441 cttgcccaga agaatgggga gcgccgaatt atcttcattg atatccaatt cgaattcatt
    19501 ttgttggaat gtgacaggca ggttacggcc acagatgaac ttctgacggt tatcctccca
    19561 tttccacacg gaggcactat cgtaagggat gacgcgcctg atctggtcga ggatgttttc
    19621 gacgatctcc aaaaattcga acatacccac gatgcctgcc aggctttcac gcagggtttc
    19681 agattctgcg tttttcgccg ccagtttctg aataagggtc tcgcgttcgg cttccacttt
    19741 tttgcggtca gtgatgtcac gctgaatgcc aaagaaaccg gtgatccatt ttcgttcgtc
    19801 atacatgcag acgtagtccc cttcaaacca gaccggcgtg ccatcggtct tgcgttcata
    19861 ggtttcaagg tgcaggtgac cttcgtcgaa tagtttcctg cgcaggttca acccttgctg
    19921 caagtcgtgg gtaaaaatat ctctggaggt gagcgagaga aaatggtggc gggtgatctg
    19981 gtattgttcc aacatggcgt tattcacatc agtatagcgc tgggtattga agatatagag
    20041 aagtatttct tctttatcag cggcatccgc ccagggcagg ggtttctcca gtatgcaaaa
    20101 gaagaagcca tcaagtgact ggttaaaaaa tgcctgcaac cgttgctggt tttgccgcag
    20161 ggtcatttcg gccagttttt tttctgtaat atcttcaact gtcccttcaa aataaagcgg
    20221 accgccgttc ttatcccgga tcaactttgc attctcgcgc acagaaaaga aggtgccatc
    20281 agatctcatc caaagcgttt caaaatcacg catctcccca tccctttgga tcgcctgcaa
    20341 aaattcctta cgcccataat cggtaatgat gggttcacct gcctgatctt gttcgagcaa
    20401 ttccgccaca ctctcgtatc caagcatgcg ggccagcatc gtattggcaa ggagaatatt
    20461 ccccttcagt gaaatacgat aaaggccgat ggtggcgcct tcaaacacac tgcggaaacg
    20521 ctcctcgctt tcacgcaaag cctcttccac cagtttgcgt tcggtgatat cgcgcacatt
    20581 tgcaacaata accatattat cgccgacatc aagcatgcgg gcgctgacct ccaccggaaa
    20641 ccgtgtccca tccttccgca gataacttgc ctcaaaaata agcccgcccg attcgcgtac
    20701 aagttccaca cgctcattcc aaacctgcag atccgtgaca tgtgatgcga catggatcac
    20761 atccagttga agcaattctt cacggctata ccccagtttt tcacaggctg ttctgttaaa
    20821 gtcgatgtaa cggctggtct gcgggtcgat caaaaaaatg gagtcatttg cctcatccat
    20881 caattttcgg aattgaagaa gcctgtcttc ggcctgttta cgctccattt caaccatttt
    20941 gcgcagcgaa atatcttcaa ctgtgccttc ataatacaaa atgttaccgg cttcatcaaa
    21001 caccgtgcgg atattcgccg atacccaaaa agtagagccg tcttttcggc actcctcggc
    21061 ttcaaacccc acaaccgcat actctgtact taatttctga acaagaacat cccgttgttc
    21121 cggtgacaca tagatctgct ttgagatatc ctgcaccata cgcagcattt cttccgggga
    21181 ttcatacccg tacatgcgtg ccattgagcg atttacattg acaaaccttc catccactgt
    21241 gctttgaaag atcccatcaa ttgtgttttc aaagatattc ctgtattttt cctcggcccg
    21301 cttccgctct ttcaactcct gttgcaggag gaggttgttc ttaaaaaaag cctctgtcaa
    21361 aaaacggacc atgatcgcag ttatcaacaa ggcaacagcc accgaaacga aatcacccgg
    21421 caagctttgc acaaaaggca cgggagtata aactccatat aattccccaa aaaccagcca
    21481 ggcagcacac ccaattgcaa aagcggtcaa aaataccatt gtgcgctttc ttgtcaccag
    21541 acttgcaatg atcagcaccg ccggaaagcc gaggttgctg atctggtgaa tacccagtcc
    21601 ataggtagcc aggactgtca gcaaagaaag caacaccacc gccagcaggg atgcagccaa
    21661 ctccactttt tgctggcgaa taaaccaaag cggaatcaac acaaaaggga cactagccaa
    21721 aagcagggca acagccagaa aactttgggt caaaaacgaa ataatccccc ccgaaagcag
    21781 cgacaacagg atcaaagccg tgaaccaata aaagatcctg acgannnnnn nnnnnnnnnn
    21841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21901 nnnnnnnnnn nnnnnnnnnn nnnnaccgcc ctatgcaagt ggagcgatac gacgcgggtg
    21961 atcatgttac ttatgagatc aacctgatca cacccgatgc cacccataaa aacgttaaag
    22021 tatccctgtt tatcgaaaga ttcgtagggg gtgggtttgc aggccaggta tataaaacgc
    22081 gcattctaaa agtggatggc gaggaaagcc ctctccaagt cggccagacc tacgccctaa
    22141 agatattcat cccaccatca caccatgcgc ttttcttccg taatcttctg tatgccatcg
    22201 ggtttcaagg tccgtttcaa atccagtgta accctgtcgc agcgcgggcg ggcgcacttt
    22261 ggcaaaaatt tatccgccgt gcagccagtc tgcgttttca agacacagat gcagttaatg
    22321 atgtgcatgg aacgttcatc gactacgatt taggcagttg cggggagatc agcaattggg
    22381 ttgaaggccg cacctggcgg ctggaagtga acgatcatgc cgacctgctg aatcgctggg
    22441 aaaatggaaa acttgcagaa ggcacctcca tcggctcttt ggaatatcgc acaaaaaaaa
    22501 ctttcatgca tgaattctct gcactgctgc atgagatggg tgcccacgaa ttcgccagac
    22561 aatacgaatg gtccacctgg aaaagccagc ccaatgtcct caaacgcctc aatacagacc
    22621 ttgaacctgc tcgcggatta acagcagtgg attttcgggc aggcctgact ctgttgccct
    22681 tcctgcccat gagtccaggt gacatcaaat tgatcttgca aggaatcaaa cgcggcagtc
    22741 tggtgcaatt tgaccgcggc gatgtaaacc agcttgaatc ctttatcaag aaaaattccg
    22801 ccgatttctc agacctgctt ccgctgttgg aagaattaaa gacatgcgaa ctggtctatc
    22861 gaaactccat tcctgatatc acccaccacc atctaaacct catccgggac aaagcactac
    22921 gcgccaccat cgtcaacagc acgatcacag gctggagcgt aaaaaagatc gttgatacag
    22981 acgcggaaaa acgcctgcat gcaaacaagt cctttttcct gttcttcata ctgttaggct
    23041 tcatcccgtt cttggggaaa atccttcggc tcgctttcgg acgtcaggat tatcgggatc
    23101 attataaaaa aatgttgtcc agctttacgt atttccaacg cgccatgttt gccaaccgca
    23161 tggaggcctt gattcgctgg tatcgcgggg aacgcatcac agaaacattg gctgaaagaa
    23221 tatcttcatc cctgctgctt tatctttatc atatccccct ttccattttg cctaaaaaaa
    23281 ttcaccgcct gctctcagac cgtggattct ttatggacac tttatacaac accatcattc
    23341 ggcctgtgcg gttgtatttc aaccccgtgc tgcgggaaga atggctaaga gaaattgtta
    23401 gcgaaagcag agacaggcag gtactttcca aagaagatgc cgaagagata ttggggcaaa
    23461 ttcacgaccc gtatattcac aaatatttgc aaagccttgc agtccacatt gccatggctc
    23521 ccatcacaca cgtcatctcc accgggcttg cgattctcta tttattgaat catccggaga
    23581 ttccgcgagc tgaagcctac gccaccgccg ccgggattct ggccttcttt caagtcattc
    23641 ctgtttcgcc gggctctttt gcgcgcggat tatatgtcct ctttttagcc atcaaagaaa
    23701 gaaacctcaa agactataac ctcgccctgc ccctctcctt cttcaaatat gttgggtata
    23761 tttccttccc gatccaaatg acctatagct accccacact ggctcgttgt atggccgggt
    23821 tctgggccac ccgcatggca cgggtcattc cagtttttgg cgaaggcggt gccctgttgg
    23881 aatataaaac cttcaatatt ttctataact ggccgcttac cattcgtcgc aaaatgaatg
    23941 aacgggctga atggcgtaaa acgcaaaaga cccgttcatg gcacatcatc ccgattgtgg
    24001 cgttaatctc cgctgcaagc tggctgttgc aaaaactcca catctctacc acgggaatgc
    24061 ttccgccttt tgcgcttgtt gctcccagcc tgatcttgtt tgggctactc ggcgggattt
    24121 tggtaaatac aggcagtggc ggctcctctt tttcaagaag ggtgctgatg gccgttggaa
    24181 gtggagtgag catcgggttt ctggccgcct tgggactatt gattctcagc cctgaagtag
    24241 aaaccggact gatcgcatgg atatctacga tcatttggta ttccttcatt acggccacat
    24301 tcagcaccac cagcgcggtc atcaccgaat tcaaagtgta gagagccttg ccaggtagtt
    24361 gatcgaaata aattttatag attcttgaaa actcaaaatt gcgagaaatc aaaagacttc
    24421 tcgcaatttt gagttttatt gaccgttatg aaatccagat catgaactgt tctttgatca
    24481 cccattcccc attgaccttt tccatcagaa agatatcgcc gctgcccatt aaaggcccag
    24541 ccacattgcc cacataaata accgcttggt caagagtacg attgaagccc acccgggaaa
    24601 agatcaaata cccatacgaa ccgggatatc tctctgcaag ggtttcaccc caatcaggct
    24661 ggctggagat ctcggccaat tctttttcac tcagcagaac ataatccacc cccaactcca
    24721 tctccgccga aagctgaccg ggttctgcat ttcgttcaac aaaactttcg atcgtatcac
    24781 ctgaaatatt ctggaaagta tcctgaagtt gccgcctcat ttcctgaggg gtcgggttgc
    24841 cgatcccggc ggatgtgttc tcaagaatca ccgcggaact ggtacctgca aaaaaggcat
    24901 aaatggcctc ctcttctttt tcgatctcag acgcactgag ggctggtttg ggcgtcaaag
    24961 tggagcacgc caaagcaaac aggatcggca caaagactaa cgttcgtttc aaagcattcg
    25021 cagacatgat tcaattctcc tttttgaatt ttctcaaaga catcctgcac gatctgattg
    25081 ttccatctgc aggtaggata ttggtcatgg gcatccctgt cagtttcaaa aaggacggat
    25141 atcttttaga tatccgtcct ttttgaaatc tattggattg agatcgaaaa ccgggtctca
    25201 gttgtgtatg actcaccctg tgcaggcgga taatttccta aaacaagaac cgtctctcct
    25261 gcagaaactg ccttgagtgt ccacacttcc acaccgccgg aaccgggcat cacaggttct
    25321 gaagcggtat attcgcgtga aacaagctcc aaatgggcat catccaatgg cccgatcatt
    25381 tcccaatgat aaccagtgga aggattggat tccattacga tcatgaaggt gtctcccgct
    25441 ttcacagtga tcggttgaga tgggtctgta agagcaggca gggtggacga gatcggggct
    25501 tccgtaggca gcgaggtggc aggcgcagat gcacaggccg aaaacagaaa tgctgttacg
    25561 aacatagtta gtaaaatacg tttcatggga actccttgag ttttttggtc aaatggcaac
    25621 ggcatttcga cgccaaattg aattgatttg ttcccaaccg gaaagtctta attttcaggg
    25681 caaataaagt gacctgcttc tggtattctc ctgtttattt cagtaggcgc ggacatcaat
    25741 ttcgtaaaac ttgtaacagt tccgcgtcct ttttgcctga tacatggcga tatccgcctt
    25801 acggagaagg gtggcggcat tgtcattttc aggcgataaa aagctgattc cgatactggc
    25861 agtcaccttc aaaaccttgc cgtcgagttc catgggttga gagagtgttt ccagcacctt
    25921 ttgtgcaatg gaactgcagg tttcaagatc gttggcttct tcaatgatca gtgtgaattc
    25981 atctccgccc attcgtgccg ctgtatccga ttttcgcagg caggtttgca gccgcaatgc
    26041 agcaaccttc agcaaatgat ctccatagat atgcccgtgc aggtcattga cctctttgaa
    26101 gttatcaaga tcaagcagca tcaccgcaag ttttccatac ggcttgcgcc cggcacggcc
    26161 aagggcaatt tccaaacggt cttgaaaaag ctggcggttt ggcaggtcgg tcaacacatc
    26221 atgcatggca aggtattcaa gccgccgctc tgcctgcttt cgctcggtaa tatcgcgtac
    26281 gatcccccca atgcgataac cgagatccgt cttataggtg tacatcatga tctcaacatt
    26341 gcgatagatc ccatctgaat gttgaaagac tccctctcgt ggcaggttca ggcccgggcc
    26401 aagtccagat gcgagaatct ccttaaagtt ttcctccagt ctggcgaaca tgacatgggt
    26461 ccgcaattca tcaggcatca ggttccagtg aatttcccag atcggttttt ccagggcctc
    26521 atgtcgtggc agaccagaga tggcttcctg gccgcggttc cattcaacga caagcccatt
    26581 ttcatcggcc ataaagattc catcaggtga ttgttccacg aggctgcgaa atctttgctc
    26641 actgttgatc agggcttgct cagtttgttt gcgttcgctg atatcccgta acatcccgat
    26701 gatctgaccg ttggaaagca gtttaccggt gatctcaaca ggaatacgga cgccgtcttt
    26761 tcgaataaag aaactttccg tcatgaccag cttatccact gccagttgag ataacttttc
    26821 ttgaatatgg gggagttcat cttcaggttg aaaactcaat acgggctgcc caataagttc
    26881 ctctcttgaa tagcccaaaa tctcgcagcc ccgcggattc atttcaagca tacaactttg
    26941 agcgtcaatc agaaaaatac catcccccgc ctgcagaaag atgttttgaa aaacctcgtc
    27001 tgaaagttta taacccggct tagacgcacc atgcggcata accaacctcc tgaaacattt
    27061 aaggcatggt tcatcacgtg aacgccacga tgattgcgtt agattttacc cttgatattt
    27121 aaaataccct caaaactgat atttatgacc tgataacagt gaaatcaaag ccgaaatctt
    27181 cattgagcga aagtttttat agcgtcagcc tgattaatac aattgacctc ttgcataagt
    27241 aaagtgttgc agaccacaaa gtcaccaagg cactaagctt tttttctttg agtcttcgag
    27301 actttgtgcc tcacctgcac tagcgtgcgg cgca
//
LOCUS       DAOQJC010000052        25338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_859_length_25338_cov_9.868014, whole genome shotgun sequence.
ACCESSION   DAOQJC010000052 DAOQJC010000000
VERSION     DAOQJC010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 25338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25338
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_859_length_25338_cov_9.868014"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..528)
                     /locus_tag="PLE39_09665"
     CDS             complement(70..528)
                     /locus_tag="PLE39_09665"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HNE68636.1"
                     /translation="MIYAIAKWIHILCAIISLGANLTYFPWFMTVPKTRETLVYTLKT
                     IRLLDNWIANPAYILAYVTGEIMMRVGGQIHYSTPWMTVALILYVVISILGLFVYAPM
                     LKKQQQLAETKGADNPDYQRVSRNGIILGTVIVIITIGITYLMATKPQLW"
     gene            complement(583..>2388)
                     /locus_tag="PLE39_09670"
     CDS             complement(583..>2388)
                     /locus_tag="PLE39_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE68637.1"
                     /translation="VSTLAMKNPFTYQYAKEDWDKSYWNDPAFIRINEIITSVWAGIF
                     TVNTLLGVLAVAIPAQKLIWAVIVPNVGVVAGIIFSSRFPAYYSRKSVQARLDAWDPY
                     KWSAPKFDKRPADNNEHDVIVIGSGIGGLSAAALLAKRGLKVAVFEQHYLPGGFCTAW
                     DRNVRIPGQPIESRERWTYIFDAGVHDFSGLGERGGIRSLLRMLDIENSIEWKLNEQE
                     YFLGETHFKVPHDANEFIQQLGERFPSEAQNIRAFFDEMLGVYRDMYADMEKLGGAPR
                     TPDNVDDLLAYPRTHPHSYKWMDRPFIEMLDTFFKDARVKELLCALTGYLSDDPKSLS
                     VGNMAPIFGYHFDGGYYPVGGSQAFPNALVDAIEKHNGKVYLRTPVKRILTEKGRAVG
                     IELKDGTTHHAKALISNADTHKTFFELLGREHLSADFTNYIDSLKPSTTAFIVFLGLD
                     ITPNLAPITMLDDIGIMIPSNVDPSLAPPKHASVTLLKLLPESEFAKWDRKSEGYNQR
                     KRAYADQMIASAAEVIPGLQQHITYRQEGTPATFARYAWTTNGSIYGPSMGQKRLTVK
                     TPVKNLYVAGSGVMGGGVEAAVIAGIHAANEIYKN"
     assembly_gap    2389..2488
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2614..3183)
                     /locus_tag="PLE39_09675"
     CDS             complement(2614..3183)
                     /locus_tag="PLE39_09675"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNE68638.1"
                     /translation="MPLEHAILAFLEYNPMSGYDLKKLFDVSVAHFWSATQSHIYKSL
                     EGLEKKGWAEAQIIPQEGKPNRKEYQITAEGRIELRRWLVTPIPLGPVREACLIQIFF
                     SHFSTNEEIAGLFETRMKEIREHLHILKTVAQTAIDENAKQVGVERARQLWQITLDYG
                     IDYYEFELAWHEKTLKKIHNLPPLTLPNS"
     gene            3331..>4526
                     /locus_tag="PLE39_09680"
     CDS             3331..>4526
                     /locus_tag="PLE39_09680"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNE68639.1"
                     /translation="MNTPVIIDAIRTPIGALGGILSSVRPDDMAAHVIKAILERNQID
                     PALIEEVFLGCANQAGEDNRNVARMAALLSGLPLEVGGVTVNRLCASGLNAINMASRA
                     IKAGEGDIYIAGGVESMSRAPYSLPKAEAGFSFGNLTAYDTALGWRYPNPKMKEMYGT
                     DSMGETAENIAAERPHLTREKQDEFSVRSHQLAVKAIDTGRFKQEIVPISIPQKKGDP
                     KLVDTDERPRRDTTMESLAKLKPAFKKEGGTVTAGNSSGLNDGAAALLIMSEEKAKAL
                     NLKPLARIVTSASAGVPPRVMGYGPVPATRKALQRAGLRIQDISLMELNEAFAVQSLA
                     VIEDLGIDPDLVNVNGGAIAIGHPLGCSGARLVTTLVHEMKNRGNAKYGVATLCVGVG
                     QGEATV"
     assembly_gap    4527..4626
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4627..5691
                     /locus_tag="PLE39_09685"
     CDS             <4627..5691
                     /locus_tag="PLE39_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68640.1"
                     /translation="PPQPMKKIRSFLFLLTLLTACSAPTPAIPPTLPPVENTPLPTQA
                     LQFTPIPTLTFTPVPTEGFPTFVAPTSTPDPSTRPNTIQFPANGTYVDINDFIAINTS
                     KTYTVNAMKGQIMSVSVLPQSADNNWTYIPLQIKGADGSVLCPQTESDCTAWRGVLPA
                     TQDYFITLSPATDVIQFMLRVAINPPGKSEQYFQYSNPANGVALTYSDYFAPVFPVFS
                     NYKIVPELALYMNDQETYVKTNLSEAYFFLGSSADPQMVATCTDSNPNGGAIEQIVGN
                     EVINGFTFVHSTSAGAGAGNLYEQEIYRMAYNNICYEVIYHMHSANIGNFPPDTVTEF
                     DRNAIMNKMYTVFSTLTISK"
     gene            5704..6630
                     /locus_tag="PLE39_09690"
     CDS             5704..6630
                     /locus_tag="PLE39_09690"
                     /inference="COORDINATES: protein motif:HMM:NF022114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HNE68641.1"
                     /translation="MNRNHLALVLILTLATLACGLAAPASNAPTPAPVQETVPAPTES
                     APSLTVDQLKNAQYQLGARDDHAVIQLTDGKYQQGTDATTLDFAYIGLTQFSSVGDLT
                     GDGVNEIAAIFLENYGGTGNFGLLAIYSNVNGLPVFLTSTFIDDRPMINSMSIENGEV
                     VLDAIVHGFQDGGCCPTLPTTRRYTLVNNQLRMVNYTTATPEGAKRIVEIAAPANGAE
                     ASGSVQVSGTVSIAPFENNLSYFIFDEAGNQLAAGPVMVTAPDFGAPGTFNETFSLEG
                     IPSGTTVYLEIQDISAADGSWLAMDAVKLVIK"
     gene            6664..7077
                     /locus_tag="PLE39_09695"
     CDS             6664..7077
                     /locus_tag="PLE39_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE68642.1"
                     /translation="MPHLVRDWMSSPVVVVDPDSSVSHAMTLMRRRNIHSVVVDVSEK
                     NPSYGIVTTTDIRDKIVAVNRNPAEITVREIMSGPIITGRAEWTLMECSQLMQKHKFH
                     HLPIADEHGSLIGLISATDIFMSVEEQGWEEEKKD"
     gene            complement(7203..7631)
                     /locus_tag="PLE39_09700"
     CDS             complement(7203..7631)
                     /locus_tag="PLE39_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNE68643.1"
                     /translation="MTLPTKLQEIVDDIASMSREEKLETLIAYAESMPDLPERFKDER
                     SKMEPVPECMTPVFIIAEKLESGGIFFHLDIPPQSPTVRGLASILTNGLNGCLLEEIL
                     SVPADFYIPMNLQEAVSQQRINGFIGVLAHMKQAAVKASS"
     gene            complement(7678..8517)
                     /locus_tag="PLE39_09705"
     CDS             complement(7678..8517)
                     /locus_tag="PLE39_09705"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNE68644.1"
                     /translation="MSYAHPEYLVDTEWVANHLNDANVRIIESDEDPLLYAIGHIPGS
                     VQVDWFSTLQHPLRRDFVTKEQFEEVASKLGITNDTTVVFYGDKSNWFACYALWLFQY
                     YGHANVKIMNGGRIKWEQEKRPLVKEVPSYARTTYSAKEADKSIRAFRDDIFKQIEGK
                     RPMVDVRSPKEYTGELTHMPNYPQEGATRGGHIPGAVSIPWSQAVNEADATFKTTEEL
                     VALYEGKNIKPDGEIIAYCRIGERSSLTWFVLKYLLGYKKVKNYDGSWTEWGNLVDAP
                     IEK"
     gene            8838..9461
                     /locus_tag="PLE39_09710"
     CDS             8838..9461
                     /locus_tag="PLE39_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HNE68645.1"
                     /translation="MAKKKFYVVWKGRKTGVFTTWADCEKQVKGVVGAQFKAFESEAE
                     ADAAFLKQYDDYKGKPSTHGKWKMASTKPILPSICVDAACSGSTGKLEYRGVNTETGE
                     EIFYAGPYAEGTNNVGEFLAIVHALTWQAKHNMHVPIYSDSENAIAWVATGICKTNLK
                     HSPKNAVLFAIIRSAENWLAENELPEDKIIKWDTGLWGENPADFGRK"
     gene            9562..11091
                     /locus_tag="PLE39_09715"
     CDS             9562..11091
                     /locus_tag="PLE39_09715"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNE68646.1"
                     /translation="MDVRFHFSMIFSLLITYFIFRPIDLRSTVLALLWLTGFVLCILL
                     HELGHALAAKLVGVDVKSIIIWLLGGLTSLERKPERPFHNLVIAAAGPMVNMLLGFIF
                     VAGYILLHIFAPRGLEPELFIWVQTFIELCFSLALLNVILVVFNLLPIYPLDGGNIMH
                     AVLDGLFGRSSADLITMLVSIPVLLGLVVLGLYTRDYLLLASCVLIALAIGTLNRSVL
                     RWVNLGVNYLFKRSGYYYLQGDYERAAQLYTREIEREPEQPAHYLARAACLLNVMQKE
                     RALADIDRALKLNPKNYIALELRGELYSMDKDYDSALEYFARAQELSPNWAVPHFDRA
                     SILMDKQEFGPALAELNQSISLMSQFTLFYVVRSLAHFKLGNIEAAHKDQDAALRLSE
                     KDALTVSPVNMTLYEGYLDWAEEYYERAISKLPRSFLAYQGRADAYRTNKEFEKAIAD
                     YTHAIQLAPHEADLYLGRGKCHQALNDPEKAAADFRLASEKADKLHLKRQAEELLKSV
                     V"
     gene            11163..>11721
                     /locus_tag="PLE39_09720"
     CDS             11163..>11721
                     /locus_tag="PLE39_09720"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HNE68647.1"
                     /translation="MKTALLVIDIQKDYFPGGKYELVNPLAAAKNAYMLLQCFREHGG
                     HHVHIQHISLEPDATFFIKGDSGSDIHDSAAHFEGEPIVYKHEPNSFLNTNLLDLLKG
                     WGVERVVITGMMSHMCVDATARAASDLGFKVIVAEDACATRDLQFDGTTVPADHVHKA
                     FMSALQSYGDVMKSDQVIALLAGEMN"
     assembly_gap    11722..11821
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11822..12988
                     /locus_tag="PLE39_09725"
     CDS             <11822..12988
                     /locus_tag="PLE39_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNE68648.1"
                     /translation="FNLTLPMTALDLEKQKQAKEYSRINRRLWLVDTLFGVAYALAWL
                     FLGWSISLREWLHQLVTVQWSVTTGADWLIIALYVAVFGGISAIINLPLSYYSGFVLP
                     HRFGQSNQSLKDWLVDQVKGLAIGAPLGLIMLELMYLALRLTGDAWWLWVAGGLLLFN
                     ILVSNLAPVLIMPLFNKYVPLGEEHKELEERLLALAERANTKVKGVFKFDMSKRTKAA
                     NAALTGLGNTRRIILGDTLITEFTLDEIETVLAHELGHHVHKDIPFLIAFGTVSTTLS
                     LFIASLALDSAVSFFSFTSPADIAALPALMLILTAYGLLTSPVNNAISRWRENMADDY
                     ALSSTQKGEAFASAFTRLANQNLGEIDPEKWVVFMFYSHPPLGERIEKAKRFAA"
     gene            complement(13048..13911)
                     /locus_tag="PLE39_09730"
     CDS             complement(13048..13911)
                     /locus_tag="PLE39_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNE68649.1"
                     /translation="MKIVTDCAADMPNEELERLGVVQAPLFIQFPEGEVNAMEISADE
                     FYNRLEAMRPQIPTTAQPSSGIFAELYRKLAQTEKNIFSVHISSGLSGTINSARDGGE
                     QVKAEANVSFWDTLTLSGGERFQVVAAALAHKAGWAMDKIQERLMKIREKTEVIYTLD
                     TLEYLARGGRIGRVKALAGALLNLKPVIRVDTDGKYSTVKTERTINKSIATIADHLVE
                     KYGNTPVWVTVLHGRFAEKADLLADEFRQKLNVAKMEVQRISPVLGVHTGPGIVGAAV
                     VPMELMNDLGE"
     gene            complement(14008..14592)
                     /locus_tag="PLE39_09735"
     CDS             complement(14008..14592)
                     /locus_tag="PLE39_09735"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE68650.1"
                     /translation="MQQRSEETRSKILGAAIKLFSTRGFNSASVDDICRDAGISKGAF
                     YHHFKSKQALFLALLDGWLQTIDNAIEASKDKTVPETFMQITEAFPYIFETAGEGLPM
                     FLEFWMQASRDEKIWQASIAPYRRYQKHFTQLIKKGVDEGSFVEVDPELTSRMIISTA
                     MGLLLQSLLDPKGAKWEKVARDSTNMLVNSLLKK"
     gene            14733..15728
                     /locus_tag="PLE39_09740"
     CDS             14733..15728
                     /locus_tag="PLE39_09740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE68651.1"
                     /translation="MTNIPLTPLLEKILASRSAWIDSKHESAFRLFNGFLEGCPELAI
                     DVYASTAVIHNYADDFEHGQALVEEASRFLRASLNWLRAGIVKTRHGTQEEKRGTLLF
                     GTEFDRKIKEHDTWYAINPILNRDASFYLDTRNLRKWLIENAKDKMILNTFAYTGSFG
                     VAALKGGANRVVQIDLNREFLNLAKSSYTLNGFPIRKGDFIARDFFEQVSSMKRTNTF
                     FDYVLIDPPFFSSTSKGRVDQVHESARLINKVRPLINDGGSLIAINNALFVSGDEYMQ
                     SLEALCKDGYLKINELIPIPEDFTGYPETRQGNPVTNPAPFNHSTKIAILGVKRK"
     gene            15725..16120
                     /locus_tag="PLE39_09745"
     CDS             15725..16120
                     /locus_tag="PLE39_09745"
                     /inference="COORDINATES: protein motif:HMM:NF021514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNE68652.1"
                     /translation="MSGETDLIKLLQNMKPELNTGEYVYCLADSKKQAAELEPLCFFI
                     EKEGVTMILPKEKAKAMNIPYTTVCAWITLTVHSSLEAVGLTAAVSKVLTEANISCNV
                     VAAFYHDHIFVPIADASRAMDALLSLTKG"
     gene            16122..16496
                     /locus_tag="PLE39_09750"
     CDS             16122..16496
                     /locus_tag="PLE39_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNE68653.1"
                     /translation="MNSKQVIETFWQMMEGNDFYAVAQLLHEEYTLDWPQSGERIRGA
                     ENFAKLNTAYPAEGKWRFVINSIVVDGENVVTDVSVTDGVRHDRVITFSTIRDEKIWK
                     QVEFWPEPFEAPNWRAQWVERI"
     gene            complement(16493..17371)
                     /locus_tag="PLE39_09755"
     CDS             complement(16493..17371)
                     /locus_tag="PLE39_09755"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNE68654.1"
                     /translation="MALYLGCPVWSFKGWVGNFYPKGTKSTDFLREYARRLTTIEGNT
                     TFYAVPAPKTIEAWAEQTPETFRFCPKIPKAISHNGKLADYADRARQFVEIMRPLASR
                     LGPMFLQLPPSYSPRLMDDLATFLDAFPTDVRLGVEVRHLDWFEEPHRTRLNQLLTDH
                     NMARVVIDTRPIRDLAGDESIKGSSYESLLEARERKPNVPVFEEQTTDFTFLRYIGHP
                     QMEQNQAWVEEWVNRITDQLSAGTDAFVFCHSPDNMLAPSICREIHQKISSRIKIQPL
                     PWDDIRSDVFEQGKLF"
     gene            17491..17925
                     /locus_tag="PLE39_09760"
     CDS             17491..17925
                     /locus_tag="PLE39_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNE68655.1"
                     /translation="MYKKILVPLDGSPLAEAVLPHAQALAKSEGAEIVLLSVPVTPSY
                     DYLERTPGLASQIIESAERETEAYLKAEVEKLTGEGMKVSGVMREGPIPEMILKVAEE
                     VHADMIAMSTHGRSGIQRWLMGSVADRVVHHAHIPVMLIHPN"
     gene            18240..18983
                     /locus_tag="PLE39_09765"
     CDS             18240..18983
                     /locus_tag="PLE39_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68656.1"
                     /translation="MKKNLDVKKTAIIFFVLLFAAYIPVFLKEQVLDAFVREDRIYET
                     LSPIYLFVTSSMFAVAFYRSPIKLSLKDPAWLKRLFFLAFCGLFFLAAMEEISWGQRI
                     FGVETPNLIKDVNVQEELTFHNLNVFQGDDAKLPLDFDQLSAIFALTFGFLVPFVCYF
                     FIPVKNFLGTKFPVLPLQIGLLYPVNYLIQKTLMRVLPIFPSLFQHPNMRFPQAVYEV
                     REHNYAMLLMVSAVFFILLKLDLAGDGTQ"
     gene            complement(19043..19579)
                     /locus_tag="PLE39_09770"
     CDS             complement(19043..19579)
                     /locus_tag="PLE39_09770"
                     /inference="COORDINATES: protein motif:HMM:NF016413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HNE68657.1"
                     /translation="MTNRYKIRQNLISIGDDFWIENEEGKKVFKIDGKVLRIRKTLVF
                     EDAKGKKLAQIQERLLTIKDTMVVEDADGKDIATIKKALISPFRDKWDVNVKNGPDLD
                     VQGNILDHEYTVKQGREKVAEISKKWFRLTDTYGVEIEDGQNDILILAIAVAVDMMAH
                     DDKEDKKRKKEKDEKKDR"
     gene            19797..20261
                     /locus_tag="PLE39_09775"
     CDS             19797..20261
                     /locus_tag="PLE39_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68658.1"
                     /translation="MDGRNGFDFLVGSWKVHHRRLRERLKGSSDWEEFKGDTVDRKIL
                     NGLGNMDENIMHRETGPIHAVTLRLFDPQAQEWSLYWATGISGILEVPMIGGFKHGLG
                     EFYSQEVFEGRHIFSRFVWSEITTVSCKWEQAFSADGGQTWETNWVMDFQRV"
     gene            20288..20764
                     /locus_tag="PLE39_09780"
     CDS             20288..20764
                     /locus_tag="PLE39_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68659.1"
                     /translation="MQTENDGRNDFNFFIGNWTVKHRKLKELLKNCAEWDEFESTVVD
                     RAIMGGIGNLEEMIFERTAGRFYGTSLSIFNPKTKQWSQYWVDSANATLQDPMIGKFT
                     DGVGEFFSEDEFEGRTVLARARWYDIEENSCKWEQSLSDDGGQTWETNWVMEFIRA"
     gene            20761..21483
                     /locus_tag="PLE39_09785"
     CDS             20761..21483
                     /locus_tag="PLE39_09785"
                     /inference="COORDINATES: protein motif:HMM:NF019590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HNE68660.1"
                     /translation="MSRQTHSPIVELRMYKLRKNRRDELIELFEREFIESREAVGIQV
                     LGQFRNLDDPQSFVWLQGFSDMPARAQALHAFYTSPIWMENRKAANATILDSGNVLLL
                     HPARPDSGFKLKIGARPPRGNTTPQDGFVAVTICYFDGSVTNEFIEYFESTLQPALQE
                     AGASILAYFVTEDSPNTYPRLAIREGEHVFVWFAGFRESETFAAMLKSKAWKEISKSL
                     SQHVSGDPEVLRLTPTPRSWLK"
     gene            21503..22450
                     /locus_tag="PLE39_09790"
     CDS             21503..22450
                     /locus_tag="PLE39_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HNE68661.1"
                     /translation="MYHPTTRVLAVLSLLQTYGRMSGAELAQRLEVNIRTLRRYIVML
                     QDLGIPIEAERGRNGAYVLSAGFKLPPMMFTNEEALALTVGLISVHRLNPADTGYAVE
                     SALAKLERVMPLDLKSRVRALTETITLDLKTESSIPTNEVILSALSSAAQLQKQVHIQ
                     YRPDQGEETERDFDPYGLTYFQNKWYVIGFCHLRESLRSFRLDRISGIKTLEAGFSRP
                     DGFDALGHIMQAIATLPRKYPFEIVLKTDIETAQREVFDVLGVLEAGRDGIILRGSVD
                     DLDWLARQLSIFSFGFSVRTPKELKTALKQHAVRLGNVI"
     gene            22625..23836
                     /locus_tag="PLE39_09795"
     CDS             22625..23836
                     /locus_tag="PLE39_09795"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE68662.1"
                     /translation="MTDAPSPLIFISYSRVDSKAQQRLADALRKHGLTVWVDNEKLTP
                     GNPAWEREIEKAILSCSAVIAILSPEAKRSEWVRREISFAEQHNKRIFPVLLRGDESS
                     TMTLRMINHQFVDLRQNEKEGVEVLFNTISEYLEYVKANPSGEGAGSNSLFSKPKAWI
                     FAVLGIFALLMTTSFLLKMGPFTVPATPTATTPPTATATIPPTATAQPEVVIPEFFTV
                     DFDTDLENWDTFLVGDPENEVKTSVEKSYMKFELSGKNQHYYTYYTLRTYNNVRVDIR
                     SENRGEYNSVATVICRYHIDKDWYEFNFDSGGLYQILYVKWNDDKTKTISTLIANGGS
                     SLINAGKNVNDYSVICNDRTLSLWINGNEISTVIDNRFVLEEGYVGIGVSSGTLIPIE
                     NRIDTLTISQP"
     gene            23960..24808
                     /locus_tag="PLE39_09800"
     CDS             23960..24808
                     /locus_tag="PLE39_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNE68663.1"
                     /translation="MDSLSERRKAILFLALAAILWSTSGLFIKILDWQPLSILAARSF
                     FAAIVFLVYLRRIPMRFNLWQLLAAGMFILTQFLFITSTKLTTAANAIFLQYTAPIYV
                     VLLAFWFLHEKPSRVDWLSMLIIFIGLTLFFGDKLSTEGFYGNLLAILSGVTSAVMMV
                     SFRAQKDGNPAESNLIAFLITAAFGFPFVLKETWTVESWSILAFLGVFQIGFAFIFFT
                     QGIKHIPALEANLIGTLEPVLNPIWVFLFYGESMGAYALIGGLVVLGGVVLSAVGSAK
                     AVKDRH"
     gene            24942..>25338
                     /locus_tag="PLE39_09805"
     CDS             24942..>25338
                     /locus_tag="PLE39_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68664.1"
                     /translation="MTSNHTRNPGTPLDLDWVMGAHINKSAVERRTATITGRRTVKKD
                     WQAAWLLRAVTCIDLTTLAGDDTPGRVNRLCAKAKQPLRPDMMEKLGLNGERITVGAV
                     CVYPNRVADAVLALQGSTIPVASVATGFPA"
BASE COUNT         5909 a         6261 c         6342 g         6526 t
ORIGIN      
        1 aagatgcccg caccagcaag aatgacaaga agaagggtga gaaataaaat catataacct
       61 ctttgtggtt taccataact gcggtttggt tgccatcaga taagtgattc cgatggtgat
      121 gatgacaatg accgtgccta aaataatccc gttgcgcgaa acgcgttggt aatcgggatt
      181 gtccgcgcct ttggtctctg ctaattgctg ctgcttcttg agcatgggag catagacaaa
      241 aagaccgagg atggagatga ccacatacag aatgagcgcc acggtcatcc acggcgtgga
      301 gtagtggatc tgcccgccga cgcgcatcat gatctcgcca gtgacgtatg ccagaatata
      361 agcagggttg gcgatccagt tatcgagcag gcggatggtt ttgagggtgt acaccagcgt
      421 ctcgcgggtt ttgggtacgg tcatgaacca cgggaagtag gtcaggttcg cgccgagcga
      481 gatgatggcg cagagaatgt ggatccattt ggcaattgcg taaatcattt tgacacctcg
      541 tttgagtttg gaagttgaag gttacaggtt gaaagttgca agttagtttt tatagatctc
      601 attcgcggca tggatgcctg cgatgacagc cgcttccacg ccgccgccca tcacgcccga
      661 gccagcgaca tatagattct tgacgggtgt tttcacggtc aggcgttttt gtcccatgga
      721 aggaccgtag atcgagccgt tggtcgtcca tgcgtaacgg gcaaaggtgg cgggcgtacc
      781 ttcctgccga taggtgatgt gctgctgcaa gccagggatg acttccgccg cggaggcgat
      841 catctggtcg gcgtatgcgc gtttgcgttg gttgtagcct tcggactttc tgtcccattt
      901 ggcgaactct gattcgggga ggagcttcaa cagggttaca gaagcatgtt tcggcggggc
      961 aagagacgga tccacattcg aggggatcat gatcccgata tcatccagca tggtgatggg
     1021 ggcgaggttc ggggtgatat ccaatcccaa aaagacgatg aaggcagtgg tggatggttt
     1081 gagcgagtcg atgtagttag tgaaatctgc agacagatgt tcgcgcccaa gcaattcaaa
     1141 aaatgttttg tgcgtatctg cattggagat gagcgccttg gcatgatgcg tggttccatc
     1201 tttgagttcg atgccaacgg cgcgaccttt ctcggtcaga atgcgtttta cgggtgtgcg
     1261 caaatacacc ttgccgttgt gcttttcgat cgcatccaca agtgcgtttg gaaatgcctg
     1321 cgaaccaccg acgggataat agccgccatc gaagtgataa ccgaagatgg gcgccatgtt
     1381 gccaacgctg agcgacttgg gatcatcgct gagatagcct gtgagcgcgc aaagcaattc
     1441 tttgacgcgg gcatctttga aaaacgtgtc gagcatttca atgaaaggtc ggtccatcca
     1501 tttgtaggaa tgcgggtgag ttcgcggata cgccaacagg tcgtccacgt tatcgggtgt
     1561 gcgcggtgcg ccgcccagct tttccatgtc ggcatacata tcgcgataaa cgcccagcat
     1621 ttcatcgaag aatgcgcgga tgttttgcgc ttcggatgga aaacgctcgc ccagttgctg
     1681 aataaattcg ttcgcatcat gcgggacctt gaagtgggtc tcgcccagaa aatattcctg
     1741 ctcgttgagt ttccactcga tggaattctc gatatcgagc attcgcagca gcgaacggat
     1801 tccgccacgc tcgccgagtc cgctgaaatc atgaacacct gcgtcgaaga tgtatgtcca
     1861 gcgctctctc gattcgatgg gctgaccagg aatgcggacg ttgcgatccc atgcggtgca
     1921 gaatccgcca ggcaggtagt gctgttcgaa gacggctact ttgagtccgc gtttggcaag
     1981 caaggcagcg gcagaaagac cgccgatccc tgagccgatg acgatgacat catgttcatt
     2041 gttatccgca gggcgtttat caaatttcgg cgctgaccat ttgtacgggt cccatgcatc
     2101 gagacgtgcc tgcacgcttt tgcgggaata atatgcaggg aagcgcgaag agaagatgat
     2161 ccccgccaca acgccgacgt ttgggacgat gaccgcccag atcaattttt gtgcagggat
     2221 cgcaactgcc agcacaccca gcaaagtgtt gaccgtgaaa attcccgccc atacgcttgt
     2281 gatgatctcg ttgatccgaa tgaaggcggg gtcgttccag taggatttgt cccaatcttc
     2341 ttttgcgtat tgataggtga atggattctt catcgccagt gtgctgacnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnta gatgatccag gggatgaaac cgacgaatac
     2521 ttgaaacatg ctgttttccc ttccgtgcgc aaggcgcaca aaacacacct gccttcattc
     2581 aggcagtttt tttacttttt atttctgttt tacttatgaa tttggcagag tgagcggcgg
     2641 caggttgtga atcttcttca aggttttttc atgccatgcc agttcgaact cgtaataatc
     2701 aatcccataa tcgagggtga tctgccagag ttggcgggcg cgttccacac cgacttgttt
     2761 ggcgttctcg tcgatggccg tttgtgccac cgttttcaaa atatgcaaat gctcgcggat
     2821 ctctttcatg cgtgtttcaa agagccccgc aatttcttcg tttgtgctga aatgcgagaa
     2881 gaagatctgg atgagacatg cctcacggac tggacccagt ggaatggggg tgaccagcca
     2941 acggcgcagt tcgattcgcc cctccgccgt gatttgatac tctttcctgt tgggtttccc
     3001 ttcctgaggg attatctgcg cctcggccca gccctttttt tccaaccctt ccagcgattt
     3061 gtaaatatgg ctctgagtag ccgaccaaaa atgggcgaca gaaacatcga agagcttttt
     3121 caggtcgtac cctgacatgg ggttgtattc taaaaaagcg aggatggcgt gttcgagggg
     3181 catgagtgtt ccttttgttt tatataacta cacaggaata ttatcaagca ggattatatt
     3241 tgtcaagggg aaattacctt ttctgtgaaa cagctcactt acaatggcgg gtggtaaaat
     3301 cgctttgcaa actcacatgg agaaaatcaa atgaacacac ctgtaatcat tgacgcaatc
     3361 cgcaccccga ttggcgcttt gggaggaatt ctctcttccg tccgccccga cgatatggca
     3421 gcgcatgtca tcaaagcgat ccttgaacgc aaccaaattg atcctgcctt gatcgaagaa
     3481 gtttttcttg gctgtgccaa tcaagcaggt gaagacaacc gtaacgttgc gcgtatggcg
     3541 gcgttattat cgggtctgcc gcttgaagtg ggtggagtga ccgtgaatcg tttgtgcgct
     3601 tctggtctga atgccatcaa catggcgtct cgtgccatca aagcagggga gggcgatatc
     3661 tacattgcag gcggagttga atcaatgagc cgtgcgccat attcccttcc aaaggcagag
     3721 gcgggtttct cgtttggaaa tctcaccgct tacgacactg ccctgggttg gcgttacccg
     3781 aacccgaaaa tgaaggaaat gtacggcacc gactcaatgg gtgaaaccgc cgagaatatt
     3841 gcggcggaac gcccgcacct cacccgcgaa aagcaggatg aattctcggt ccgttcgcac
     3901 cagcttgcag ttaaggccat tgacacggga cgtttcaaac aggagatcgt ccccatttcc
     3961 attccacaga aaaaaggcga cccaaaactt gtagacacag acgaacgtcc gcgccgtgat
     4021 acaacaatgg aatcccttgc caaactcaaa cccgctttca aaaaggaggg cggtacggtc
     4081 acggcgggca attcatcagg cttgaacgat ggtgcggctg ccctgctgat catgagcgag
     4141 gaaaaggcca aggccttgaa tctcaaaccg ctggcacgca ttgtcacctc cgcttcggct
     4201 ggggttccgc cgcgtgtgat gggctatggc cctgttcctg ccacaaggaa agcccttcaa
     4261 cgtgccggcc tgcggattca agacatcagc ctgatggaat tgaacgaggc ctttgccgtg
     4321 caatctctcg cggtcattga agacctcggc attgatcccg atttggtcaa tgtcaacggg
     4381 ggagccattg ccattgggca tccgcttggc tgttcgggtg cgcgccttgt caccaccctc
     4441 gtacacgaaa tgaagaatcg cggcaatgcg aaatacggcg tcgccacatt gtgcgtcggt
     4501 gtcggtcagg gtgaagcgac cgtaatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnncccc cgcaacccat gaaaaaaatc cgatctttcc tctttcttct tacgctcctc
     4681 actgcctgct cagcccccac acctgccatt ccgcccacac tgccgcctgt ggaaaatact
     4741 ccgctcccca ctcaggcgct tcaatttacc cccatcccca cgttgacctt caccccagtg
     4801 cccactgaag gctttccaac ttttgtcgca cccacaagca cgcccgatcc ttcaacccgt
     4861 cccaatacga tccaattccc tgccaacggc acgtatgttg atatcaacga ctttatcgcg
     4921 atcaatacca gcaaaactta tactgtcaac gccatgaaag ggcagatcat gtcggtctct
     4981 gttctgccgc aatccgctga caataactgg acctacattc ccttgcagat caaaggcgcc
     5041 gatgggagcg ttttgtgtcc gcaaacagag tccgattgca ccgcctggcg cggggtattg
     5101 cctgccacac aggattattt catcacactc agccctgcga ccgatgtcat tcaattcatg
     5161 ctgcgggttg cgatcaatcc ccccggtaaa agcgagcaat acttccaata ttccaaccct
     5221 gccaatggtg tagcgctcac gtattctgat tatttcgcgc ctgtttttcc tgtattcagt
     5281 aattacaaga tcgtccccga actggctctc tacatgaacg atcaggaaac ttatgtaaaa
     5341 acaaacctca gtgaagccta cttcttcctc ggctccagtg cagacccgca aatggttgcc
     5401 acctgcaccg attcgaatcc gaacggcggc gcaattgagc aaatcgtcgg aaacgaagtg
     5461 atcaacggct ttacctttgt ccactccaca tctgctgggg ctggggctgg caacctgtac
     5521 gagcaggaga tctaccgcat ggcctacaac aacatctgtt acgaggtcat ctatcacatg
     5581 cattccgcca acattggcaa cttcccgccc gataccgtca ccgaatttga ccgaaatgcc
     5641 ataatgaaca aaatgtacac cgtgttttcc acactcacca tcagcaaata gaaaggagac
     5701 tgtttgaacc gcaaccatct agcccttgtt ttgatattga cacttgcaac cctcgcctgt
     5761 ggattggctg cgcccgcctc caacgcgccg acgcctgctc ctgttcaaga gacagtccca
     5821 gcgccgacag agtccgctcc cagcctcacc gtggatcaac tcaagaatgc ccagtaccag
     5881 cttggcgcac gtgacgacca cgccgtcatc caattgaccg atggaaaata tcagcaaggc
     5941 accgatgcca caaccctcga ttttgcctac atcgggttga cccagttcag ttccgttggc
     6001 gatctgacgg gggatggtgt caacgagatc gccgccatct ttctggaaaa ctacggcgga
     6061 acaggtaatt tcggcctgct tgccatttac tcaaacgtga acggtctgcc agtgttcctc
     6121 acttccactt tcattgacga ccgtcccatg atcaacagca tgtccattga aaatggtgag
     6181 gtggttctcg atgccatcgt acacggtttt caagacggtg gctgttgccc caccttgcca
     6241 accacgcgcc gctacaccct tgtaaataat caactgcgga tggtcaacta caccaccgcc
     6301 acccccgaag gcgcaaaacg aatcgttgag atcgccgctc ctgccaacgg agccgaagcc
     6361 tcaggcagtg tgcaggtcag cggaacggtc agcattgcgc cgtttgaaaa caacctctcc
     6421 tatttcatct ttgacgaagc aggcaatcag cttgccgctg gccctgtgat ggtcaccgcg
     6481 ccagattttg gcgcgcctgg cacgttcaat gaaaccttct cacttgaagg cattccgtcg
     6541 ggaacaaccg tctatctgga aattcaagac atcagcgccg ccgatggctc ctggctggcg
     6601 atggatgctg taaaattggt tatcaagtaa actaaactgt acctacttat tgaggagaat
     6661 cccatgccgc atttagttcg agattggatg tccagccctg ttgttgttgt tgaccccgat
     6721 tcaagcgttt cgcacgccat gacgcttatg cgccgccgca acatccacag cgtagtggtg
     6781 gatgtcagcg agaagaatcc atcctatgga atcgtcacca ccaccgatat ccgcgacaag
     6841 atcgtggcgg tgaaccgtaa ccccgccgaa attacggtac gtgagatcat gtctggcccc
     6901 atcatcacag gccgcgccga atggaccttg atggagtgtt cgcaattgat gcagaaacat
     6961 aaattccatc acctgcccat tgcagatgaa cacgggtcgc tgatcggcct gatctctgcc
     7021 accgatattt ttatgtctgt tgaagaacaa gggtgggaag aggaaaagaa agactgaaaa
     7081 tcaacaaccc gcagggtacc ccctgcgggt tgttttatta gccttctcaa gccgccgtcc
     7141 tcggcggcgg ggacgccgcc gagagcatct tggttttatt ggttatgtac gcaagagcga
     7201 ttttacgaag acgcctttac tgccgcctgc ttcatgtgtg ccagcacacc gatgaagccg
     7261 ttgatgcgct gttgtgacac agcctcctgc aaattcattg ggatgtaaaa atctgcgggc
     7321 accgagagga tctcttcgag caggcagcca ttcaacccgt tggtgagaat ggaggcaagc
     7381 ccgcgcacag tgggggattg cgggggaata tcgaggtgaa aaaagatgcc gccgctttcc
     7441 agcttttcag cgatgatgaa cacgggtgtc atgcactcgg gaacaggctc cattttggag
     7501 cgttcgtcct tgaagcgttc aggcaggtca ggcatggatt cggcatacgc gatcaaggtc
     7561 tcgagttttt cctcgcgact catgctggcg atatcatcca cgatctcttg cagtttggtt
     7621 ggcagggtca taatcctcac tcattcgtcc tgaggcggcc aaaggacgaa caccttctca
     7681 tttttcaatg ggggcatcca caagattgcc ccattcggtc catgaaccat catagttctt
     7741 gaccttcttg tatcccagca gatatttcaa cacgaaccag gtcagtgacg agcgttcacc
     7801 gatgcggcaa tatgcaatga tctctccgtc gggtttgatg ttttttcctt cgtagagagc
     7861 gaccagttct tcggtcgttt tgaacgtcgc atccgcttca ttgaccgcct gcgaccaggg
     7921 aatgctcact gcaccgggga tgtgtccgcc gcgggtcgcg ccttcctgcg ggtaattcgg
     7981 catgtgggtc aactcgcccg tgtattcctt tggcgaacgg acatccacca ttggcctctt
     8041 tccttcgatc tgtttgaaga tgtcatcgcg gaatgcacgg atggatttat ccgcttcttt
     8101 cgcgctgtaa gttgttcggg catacgaagg cacttccttc acgagcggcc ttttctcctg
     8161 ctcccatttg atgcggcccc cgttcatgat cttgacattg gcatggccgt agtattgaaa
     8221 aagccacagc gcatagcacg cgaaccagtt ggacttgtcg ccgtaaaaga cgaccgtggt
     8281 gtcgttggtg atgccaagtt tcgatgccac ctcttcgaac tgctctttgg tgacgaaatc
     8341 gcggcgcagc gggtgctgaa gcgtgctgaa ccaatccacc tgtaccgagc ctggaatatg
     8401 cccgattgca tacaacaacg ggtcttcatc cgattcaata atgcggacgt tcgcgtcgtt
     8461 caggtggttg gcgacccatt cggtatcgac cagatattcg ggatgagcgt aagacatgtt
     8521 ttgggtttcc tttttctaaa gtttgtaata gcctgaaaaa taaacaagca gtcaacataa
     8581 aacaaaagga cagcgaacct tcctgcgtgg gtcaaaaaag acgcgggggg cgttccactg
     8641 gccaaagtcg ttgactgctt gttgcgggtg gtcgtcttgg gcggtggtta cgatccccgc
     8701 catggacatg tgcaatagtt ggttttcata agagttggcg tattttacgt caatcaatct
     8761 ttttgtcaag acagatattc actgagacgg gcgggtgtat ttatcgcttc ggctttactt
     8821 tgatgtaaaa ttgatgtatg gcaaagaaaa agttttatgt ggtttggaaa ggacgcaaga
     8881 cgggtgtctt caccacatgg gcggattgtg agaagcaggt gaagggcgtg gttggcgccc
     8941 agttcaaggc ttttgaaagc gaagccgagg cggatgctgc ttttctaaaa caatacgacg
     9001 attacaaggg aaaaccctcc acacatggca aatggaaaat ggccagcaca aaaccgattc
     9061 tgccgtccat ttgtgtggat gcggcttgca gcggctcgac aggtaaactc gaatatcgcg
     9121 gtgtgaacac ggaaacgggc gaagagattt tttatgcggg gccatatgcc gaaggtacga
     9181 acaatgtggg ggagttcctg gcgatcgtcc atgcgttgac gtggcaggcc aaacacaaca
     9241 tgcatgtccc catttattcc gattcagaaa atgcaattgc ctgggttgcc acagggattt
     9301 gcaaaacgaa tttgaaacac agcccaaaaa acgccgtcct ttttgccatc atccgcagtg
     9361 cggagaattg gctggcggag aacgaacttc cagaagataa gatcatcaag tgggataccg
     9421 ggttgtgggg tgagaatcct gcagattttg ggcgcaagta attttcaatt tttggcgagc
     9481 aggtttgggg tctggttgcg aagcgagtga tttattttga agtggttgtc atcctcaatt
     9541 cggctgatgc gtataggcaa gatggatgtc cgtttccatt tttccatgat tttcagcctg
     9601 ttgatcacct atttcatttt ccgccccatt gacttgcgca gtacagtgct tgccctgctg
     9661 tggttgacgg ggtttgttct ttgtattttg ctgcatgaat tggggcatgc gctggcggcc
     9721 aaactggttg gtgtggacgt gaaaagcatc atcatttggc tgttgggtgg gttgaccagc
     9781 ctcgaacgca aacctgaaag gccgtttcat aatctggtca ttgctgcagc ggggcccatg
     9841 gtgaacatgc tgcttggatt catttttgtg gcggggtata tccttttgca tatctttgcg
     9901 ccgcgtgggc ttgaacctga gttgtttatc tgggtgcaga ccttcattga gttgtgtttt
     9961 tccctggcgc ttttgaacgt cattctggtc gttttcaatt tattgccgat ctatccgttg
    10021 gatgggggga atatcatgca tgcggtgttg gatggcctgt ttggaagaag cagcgccgac
    10081 ctgatcacga tgctggtcag tattcccgtt ttgctgggtt tggttgtcct tgggctgtac
    10141 acacgtgatt atcttcttct ggcttcctgt gtgttgatcg cgctggcgat cggaacgctc
    10201 aatcgttcgg tgttgcgctg ggtgaacctg ggggtgaatt atcttttcaa acgttctggt
    10261 tattattacc ttcagggaga ttacgagcgt gcggcgcagt tatatacccg tgagatagaa
    10321 cgggagcctg aacagcctgc tcattatctt gcacgtgccg cctgtctctt gaacgtgatg
    10381 caaaaagaac gcgcgctggc tgatattgac cgtgccctga aactcaaccc taaaaattac
    10441 attgccctgg aattgcgtgg tgagttgtac tcgatggata aggattacga ctctgcgctt
    10501 gaatattttg cgcgcgcaca ggaactcagt ccgaactggg ccgtgccgca ttttgaccgc
    10561 gcttcgattc tgatggataa acaggagttc gggcctgcgc ttgcagagtt gaaccagtcc
    10621 atttcgttga tgtcgcaatt tacgcttttt tatgtggtgc gctcactggc gcatttcaaa
    10681 ctgggaaata ttgaggccgc acataaagat caggatgccg ccctgcgcct atcagaaaaa
    10741 gatgcgttga ccgtttcacc ggtcaatatg accctttatg aagggtacct cgattgggct
    10801 gaagagtact acgaacgcgc catttcaaaa ttgccgcgct catttctggc gtatcagggg
    10861 cgtgccgatg cgtaccgcac caacaaggaa tttgagaagg caattgccga ttacacccat
    10921 gcgattcaat tggccccaca cgaagcggat ctctacctcg ggcgtggaaa atgtcaccaa
    10981 gccttgaatg accccgaaaa agccgccgcc gattttcggc ttgcctcaga gaaagcggat
    11041 aagttacact tgaaacgtca ggcagaagaa ttgttgaaga gtgttgtgta agccgagtgg
    11101 ctgccttgtg agcaaatttt agcagtgcag cgccattggg ctgttttttt caaaggagca
    11161 atatgaaaac cgcattactg gtcattgata ttcaaaaaga ttatttcccc ggtggaaaat
    11221 acgagctcgt aaatccgctg gctgctgcga agaacgccta catgcttttg cagtgctttc
    11281 gcgagcatgg cggacatcac gtccacattc agcatatttc tctggagcct gatgcaacct
    11341 tttttatcaa aggtgacagc ggttctgata tccacgattc ggcggctcac tttgaaggcg
    11401 aacccatcgt ttacaaacat gagccaaatt cattcctcaa tacgaatctg cttgacctgt
    11461 tgaagggttg gggcgtcgaa cgtgttgtca tcactgggat gatgagtcat atgtgcgtgg
    11521 atgccacggc tcgtgctgca agcgacctcg gtttcaaggt cattgttgcc gaagatgcct
    11581 gcgccacccg tgacctgcaa tttgacggca ccaccgtccc cgcagaccat gttcacaagg
    11641 ctttcatgtc tgcactgcaa tcctacggcg acgtgatgaa gtcagatcaa gtcatcgcat
    11701 tgctcgctgg cgaaatgaac cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nttcaacctc acactcccca tgaccgccct cgacctcgaa aaacaaaaac aggcaaaaga
    11881 atattcccga attaatcgcc gcctttggct ggtggatacg ctttttggcg tggcctatgc
    11941 tctggcatgg cttttccttg gttggagcat ttcactccgc gaatggcttc atcaactggt
    12001 cactgtccaa tggtcggtga ccacaggcgc cgattggctt atcatcgcgt tgtacgtggc
    12061 cgtcttcggc ggcatctctg ccatcatcaa tctgcctctc agttattaca gcggatttgt
    12121 cctgccgcat cggtttgggc aatcgaatca gtcgctcaaa gattggctcg tggatcaggt
    12181 caaaggcctc gccattggcg cgccgcttgg gttgatcatg cttgaattga tgtatctcgc
    12241 cctgcgtctc actggtgatg cgtggtggtt gtgggtggca ggcggcttgc tgcttttcaa
    12301 catccttgtt tcgaatcttg cgcccgtgct catcatgccg ctcttcaata aatatgtccc
    12361 gcttggtgag gaacacaagg aattggaaga acgcctgctt gctctggcag aacgcgcaaa
    12421 cacaaaagta aaaggtgtct tcaagttcga catgtccaaa cgcacaaagg ctgccaacgc
    12481 cgcgttgacc ggacttggca acacacgccg cattatcctt ggtgataccc tcatcaccga
    12541 gttcactttg gatgaaattg aaacagtcct cgcccatgag ttgggacatc acgtccacaa
    12601 ggatattcct tttttgatcg ccttcggcac ggtcagtact accctcagcc tgttcattgc
    12661 atcactggca ttggattccg ctgtaagctt tttctccttc acctcccccg cagacatcgc
    12721 cgccctgccc gcgctcatgc tcatcctcac tgcctatggc ttgctgactt cacccgtcaa
    12781 caatgccatt tcacgttggc gcgagaacat ggccgatgac tacgccttga gttccaccca
    12841 aaagggagag gcgtttgcct ccgctttcac acggctggcg aatcaaaacc tcggcgagat
    12901 cgaccccgaa aaatgggtgg tcttcatgtt ctattcccac ccgccgctgg gcgagcggat
    12961 cgaaaaagca aaaagatttg ccgcataaaa ttgaacgggc ttgcagaaat tgcaagcccg
    13021 ttttctttcc gctttcgtct ttctttttta ttctccaaga tcgttcatca attccattgg
    13081 cactacggca gcgccgacaa tgcccgggcc tgtgtgtacg cccaaaacag gggagatgcg
    13141 ctgaacttcc attttggcga cgtttaattt ctgcctgaat tcatctgcca gcaggtcggc
    13201 tttttctgca aaacgtccat gcagcacagt gacccatacg ggtgtgttgc catatttttc
    13261 caccaaatga tctgcaatcg tcgcaatgga tttgttgatc gtccgctcgg ttttgacggt
    13321 gctgtacttg ccgtcagtat ccactcggat gacgggcttg aggttgagca acgctcctgc
    13381 caatgccttc acacgtccaa tacggcctcc acgcgcgaga tattccaaag tgtcgagcgt
    13441 gtagatcacc tcggtctttt cgcggatctt catgaggcgt tcctgaattt tatccattgc
    13501 ccagcctgct ttatgggcca aggcagcggc caccacctga aaacgttccc cgcctgaaag
    13561 ggtgagcgta tcccaaaagc tgacattcgc ctccgccttg acctgctcac cgccatcccg
    13621 tgccgagttg atcgtgccgc tcagccccga agaaatatgc acagaaaaaa tgtttttctc
    13681 cgtctgtgcc agtttgcgat acaactcggc gaatattccg ctggatggct gtgccgtggt
    13741 tgggatctgc ggacgcatgg cttcaaggcg gttgtaaaac tcatctgctg aaatttccat
    13801 ggcgttcact tcgccttcag gaaattggat gaaaaggggt gcttgtacaa cgcccagcct
    13861 ttcaagttct tcattcggca tatctgccgc gcaatctgtt acgattttca tggtttgctc
    13921 ctctttgggt tatgacggtt ttatttttgg ggagtggcta aattcgcgga ctggcaacaa
    13981 gtctgcccag aatcactttt gccactccta ttttttcagt aaactattta caagcatatt
    14041 ggtgctgtcg cgggccacct tctcccattt tgcgcctttc gggtccagca ggctttgcag
    14101 caacaacccc atggccgtgg atatgatcat gcgcgaggtc aactcagggt ccacttcaac
    14161 gaacgagcct tcatccacgc cttttttgat caactgggtg aaatgctttt gataacgacg
    14221 gtagggagca atgctcgcct gccagatctt ttcatcgcgg ctggcttgca tccaaaattc
    14281 aaggaacatg ggcaggcctt cacctgcggt ttcaaagata tagggaaagg cctcggtgat
    14341 ctgcatgaag gtctcaggca cggtcttgtc cttcgatgct tcaatggcat tgtcaatggt
    14401 ttgcagccag ccatcaagca aggcgaggaa aagggcctgc ttgcttttga aatgatggta
    14461 gaacgcacct ttgctgatgc ccgcatccct gcagatatcg tccacgcttg ccgagttaaa
    14521 cccacgcgtg gaaaataact tgatggcagc cccaagaatt tttgaacggg tttcttcgct
    14581 tctctgttgc atgatttcct gcttctaaaa tggggaggtg ataaaaaccg actggtcggt
    14641 ttgtttttac tcccgtagaa tggcaatgtc aaggtgttga atatcacatt gccagtgttg
    14701 aatgtggaag ggatataatc tcaccacccc aaatgacaaa cataccgctc acccccctgc
    14761 tggaaaaaat actcgcgtcc cgctctgcat ggatcgattc caagcacgaa tcggcctttc
    14821 gtcttttcaa cggattcctc gaaggctgcc ccgagcttgc cattgatgtg tatgcgtcca
    14881 ccgctgtgat ccacaactac gcagacgatt ttgaacacgg tcaggcgctc gttgaggaag
    14941 cctctcgatt tctgcgcgca tcgttgaatt ggctgcgtgc gggaattgtc aaaacccgcc
    15001 atggtacaca ggaggaaaaa cgcggcactc tgttattcgg cactgagttc gaccgaaaaa
    15061 taaaagaaca cgatacctgg tatgccatta accccatttt gaatcgagat gccagttttt
    15121 atctcgatac gcggaacctg cgaaaatggc tcatcgaaaa tgccaaagac aaaatgatct
    15181 tgaacacatt tgcctacaca gggagtttcg gcgtagccgc acttaaaggc ggggcaaacc
    15241 gtgtcgtgca aattgacttg aaccgtgaat ttctcaacct tgcaaaatca tcctacactt
    15301 tgaacgggtt tccgatccgt aaaggagatt tcatcgccag agatttcttt gaacaagtca
    15361 gcagcatgaa acgtaccaac accttttttg attatgtgct gatcgatccg ccgtttttct
    15421 ccagcacatc caaaggcagg gtggatcaag tgcatgaaag cgcgcggctt atcaataagg
    15481 tccgtccgtt gatcaatgat ggcggttctc ttatcgcgat caataatgcg ctctttgtca
    15541 gcggcgatga atatatgcag tccctcgaag cattatgcaa agatgggtat ttgaagatca
    15601 acgagctcat ccccattcct gaagatttta caggttaccc cgaaaccaga cagggcaacc
    15661 ccgttaccaa ccctgcgcca ttcaatcatt ccaccaagat cgcgatttta ggtgtcaaac
    15721 gaaaatgagc ggagaaactg atctcatcaa acttttgcaa aacatgaaac ccgaactcaa
    15781 cacgggcgaa tacgtatatt gccttgccga ttcaaaaaag caggccgcag aattagagcc
    15841 actttgtttt ttcattgaaa aagaaggtgt gacgatgatc ctgccaaaag aaaaagccaa
    15901 ggcgatgaac atcccctaca caaccgtctg tgcgtggatc acacttaccg tccactcttc
    15961 gcttgaagcg gtcggcttaa ccgcggcagt ctcaaaggtt ctgacagagg ccaatattag
    16021 ctgcaatgtg gttgctgcgt tttatcacga ccatatcttt gtcccgatcg cagatgcctc
    16081 gcgtgcgatg gatgccttgc tttccctcac caaaggttga catgaattca aagcaagtga
    16141 tcgaaacatt ctggcagatg atggagggca atgattttta tgccgttgcc caactattgc
    16201 acgaagaata caccctcgac tggcctcaat cgggtgaacg cattcgcggc gcggaaaatt
    16261 ttgccaaact caacaccgcc tatcctgcgg aaggcaagtg gcgcttcgtc atcaactcca
    16321 ttgttgtcga tggtgaaaat gtcgtaaccg atgtctccgt cactgatggg gtaaggcacg
    16381 atcgcgtcat cacattttcc accatccgtg atgagaagat atggaaacag gttgaattct
    16441 ggcccgagcc atttgaagcc cccaactggc gtgcgcaatg ggtggagcga atttagaata
    16501 attttccctg ttcaaaaaca tccgagcgga tatcatccca cggcagcggc tgaattttga
    16561 ttcggctcga aattttctgg tgaatttcgc ggcagatgga cggtgcgagc atattgtcag
    16621 gtgaatgaca gaagacgaac gcatctgtcc ccgccgacaa ttggtctgtg attcgattga
    16681 cccattcctc cacccaggct tggttctgct ccatttgtgg gtgaccaatg taccgcagaa
    16741 aagtgaaatc tgtggtttgc tcttcaaaga cggggacatt tggtttgcgc tcccgtgctt
    16801 cgagcagact ttcatatgaa cttcccttga tggattcatc gccagccaga tctcgaatcg
    16861 ggcgcgtgtc aatgacaacc cgtgccatat tgtgatcggt caacaattgg ttcagccttg
    16921 tacgatgcgg ttcctcgaac cagtcgaggt ggcgtacttc cacgccaagg cgtacatctg
    16981 ttggaaaggc atccaggaat gtggcaaggt catccatcaa acgcggcgaa taactggggg
    17041 gcagttgcag gaacatcggt ccaagacgag aagcgagcgg gcgcatgatt tccacaaatt
    17101 ggcgggcgcg gtcggcataa tctgccagtt ttccgttatg actgatcgcc tttggaatct
    17161 tcggacaaaa gcggaaggtc tctggcgttt gctctgccca tgcttcgatt gtttttggcg
    17221 caggcacagc gtagaaagtt gtgttgcctt cgatggttgt caggcggcgg gcatattcgc
    17281 gcaaaaagtc agtggacttt gtgcctttgg ggtaaaaatt tccgacccag cctttgaaag
    17341 accagacggg acagccaaga tatagagcca tgggcatatt atatgctttg cagaagatgt
    17401 gtctattgtc acttgaaaat tccccccgcc gtgctatggt atagatgagc ttattcaaaa
    17461 gattttcttt tcagataaag gagatgtacc atgtacaaaa agatcctcgt tccattggat
    17521 ggctcgccgc ttgcggaggc agtgttgccc catgcgcagg ctctggcaaa atcagaaggc
    17581 gcggagatcg tgcttttgag tgtgccagtc accccaagct atgattatct cgaacgcaca
    17641 cctggtctgg ccagccagat catcgaaagt gccgaaaggg aaaccgaggc gtatctcaag
    17701 gctgaagtgg aaaagctgac aggggagggg atgaaggtca gcggtgtgat gcgtgagggg
    17761 ccaattcccg aaatgatctt gaaggtggcc gaagaggtcc atgctgatat gatcgccatg
    17821 tctacacatg ggcgctctgg aattcaacgc tggctaatgg gcagcgtggc agatcgggtt
    17881 gtgcatcatg cccatatccc tgtcatgttg atccacccaa actagggcaa atacaaaggg
    17941 gtgtagattt cattagaatt ttgatttgag gtcaatgggt ttttgcggta tttcccccaa
    18001 aagcccattg ttttttttgt gattattttt gaatagtacc aaggtttttt ttgggttctg
    18061 atgttcgaaa tttaattaat gctatacttg cctcggttaa tgggagagtt ttatgcaagg
    18121 tgattttttt tggatgctga tatgaatttg atctcacata gcaattaatc aagtccgttg
    18181 aatatgccct tctcctgcca tctgcataat caataacttt aaaacgaata aggtgacgaa
    18241 tgaaaaaaaa tcttgatgtc aagaaaacag cgatcatttt ttttgtattg ctgtttgccg
    18301 cctacatccc cgtatttttg aaggaacagg ttttggatgc gtttgttcga gaggatcgaa
    18361 tttatgaaac cctttctccg atttacctgt ttgttacttc cagcatgttt gcagttgcat
    18421 tctatcgatc gccgatcaag cttagtttaa aagaccctgc ctggcttaag cggcttttct
    18481 tccttgcttt ttgcggtttg ttctttcttg ccgccatgga ggagatcagc tgggggcaga
    18541 ggatttttgg ggttgagacc ccaaacctga tcaaagatgt gaacgtccag gaagaattga
    18601 cttttcataa tttgaatgtg tttcaaggag atgacgccaa acttccgctg gattttgacc
    18661 aattgagtgc gatcttcgcc ctgacctttg gttttctggt gccattcgtt tgttactttt
    18721 ttattcctgt aaaaaacttt ttgggaacga aatttcctgt gttgccgctg caaatcggcc
    18781 tgttatatcc tgtgaattat ctgatccaaa agaccttgat gcgggtcttg ccgattttcc
    18841 cgtcgctatt tcagcatccc aatatgagat ttccgcaggc cgtttatgaa gtccgtgaac
    18901 ataattacgc catgctgttg atggtttcag cagtgttctt tattttgttg aaactggatc
    18961 ttgctggcga tgggactcaa taaaaacttt cctttatcca agaaaaaaaa tatgccgcaa
    19021 tcgcggcata ttttttttga atttacctgt cctttttctc gtccttttcc tttttgcgct
    19081 tcttatcttc cttgtcgtca tgcgccatca tatccactgc aacggcaatg gcaagaatga
    19141 gaatatcgtt ttgcccatct tcgatttcca cgccgtatgt gtcggtcagg cggaaccatt
    19201 tctttgaaat ttcagctact ttttcccgcc cctgtttgac cgtatattca tggtcgagaa
    19261 tgttcccctg cacatcaaga tcggggccgt tctttacatt gacatcccat ttatcacgga
    19321 agggggaaat caacgccttc ttaatggtgg caatgtcttt gccatcggca tcttccacca
    19381 ccattgtgtc tttgatggtc aacaaccgtt cctgaatttg agcgagcttt ttgccttttg
    19441 catcttcaaa caccagtgtc ttgcggatgc gcaacacttt gccgtctatt ttgaagacct
    19501 ttttgccttc ctcgttttcg atccaaaaat catctccaat ggagatcagg ttttggcgga
    19561 ttttgtaacg atttgtcatt tgagcacctc tttttacgga tgaacgaact ttcatggtga
    19621 gtatactcga ttggattttt cagcatgttc atgctgtatg ccgattggat tgcaaaaata
    19681 tgtgatgaac gtgggttatt ttattaagaa atccctttta aattaggaac gatcctgtcc
    19741 gcatttcttc ctatactgag gctattgtac taaccacttt ataaggagca taaataatgg
    19801 atggaagaaa tggttttgat tttctggtcg ggtcctggaa ggtacatcat cgcaggttga
    19861 gagagcggct gaaagggtcg tctgactggg aggaattcaa gggcgatact gtggatagaa
    19921 agatcctgaa cggccttggc aacatggacg agaacatcat gcaccgtgag acggggccga
    19981 tccatgctgt gaccttgcgg ttgttcgatc cccaggcgca ggagtggagt ttgtattggg
    20041 ccacaggcat cagcgggata ttggaggtgc ccatgatcgg cggcttcaag cacggacttg
    20101 gcgaatttta ttcgcaggaa gtctttgaag gccgtcacat cttcagccgt ttcgtttggt
    20161 ctgagatcac aaccgtttcc tgcaagtggg aacaggcatt ttcagccgat ggcggccaga
    20221 cctgggaaac caactgggtc atggatttcc agcgcgtata gattcaacat atttcaagga
    20281 gccgaacatg caaacagaaa atgatggcag gaacgatttt aatttcttca tcgggaattg
    20341 gacggttaaa caccgcaaac tcaaagaact tttgaaaaat tgtgccgaat gggatgaatt
    20401 cgaaagcacc gttgtagacc gtgccatcat gggcggcatt ggtaatcttg aagaaatgat
    20461 ctttgaaaga accgcggggc ggttctatgg cacttccctg agcatattca acccaaaaac
    20521 aaagcagtgg agtcagtatt gggttgatag cgccaatgcc actttacaag acccgatgat
    20581 cgggaaattt accgatgggg tgggtgagtt cttttcagaa gatgagtttg aaggtcgaac
    20641 cgttcttgct cgcgcacgtt ggtatgacat cgaagagaat tcctgcaagt gggagcaatc
    20701 tctttcagat gacggcggcc agacctggga gaccaattgg gttatggagt tcatccgcgc
    20761 atgagcaggc agacccattc cccaatcgtt gaattgcgca tgtacaaatt gcgaaagaac
    20821 agacgcgatg aactgatcga actctttgaa cgtgaattca ttgagtctcg agaagcggtg
    20881 ggtattcaag tactcggtca atttcgcaat ttggatgatc cacaaagttt tgtatggctg
    20941 cagggattca gcgacatgcc tgcccgcgca caggcactcc atgcgtttta taccagtccc
    21001 atttggatgg agaaccgcaa agctgccaat gccaccattc ttgactcggg caatgtactt
    21061 ttgctgcacc ccgcccgccc cgattctgga ttcaaattga aaataggtgc tcgtccacca
    21121 cggggcaata caaccccgca agatggattt gtagcagtga cgatctgtta ttttgatggg
    21181 tctgtcacca atgaattcat tgaatatttt gagtccacgc ttcagcctgc attgcaggaa
    21241 gcaggggcat ccattctggc ttactttgtc actgaggata gcccgaatac atatccaagg
    21301 ctggcaattc gcgagggcga acatgttttt gtttggttcg caggctttcg cgagtcagaa
    21361 acgttcgcag cgatgttaaa gtcaaaggct tggaaggaga tatccaaatc gttgagtcaa
    21421 catgttagcg gtgatcctga ggtgttgcgg ctcactccca ccccgcgttc gtggctgaaa
    21481 taaaacgtgt atactcgcgc gtatgtatca ccccactacg cgtgtgctgg ccgtgttgtc
    21541 tttgctgcag acctacgggc gcatgtctgg tgcggagttg gcccaacggc ttgaggtcaa
    21601 tatccgcact ttgcggcgct atattgtgat gctgcaagac cttggcattc ccatcgaggc
    21661 ggagcgcgga aggaacggcg cgtacgtact ctctgcggga ttcaaactgc cgccgatgat
    21721 gttcacgaac gaggaagcgc tggcgctgac ggtgggtctc atctctgtgc atcgcctcaa
    21781 ccccgccgac acagggtacg ctgtcgaaag tgcattagcc aagctggagc gggtgatgcc
    21841 cctcgacctg aagagtcgtg tccgcgcgtt gacggagacc atcacgctcg atctcaaaac
    21901 tgaatcctct ataccgacga atgaggtgat cctcagcgcc ttgagcagtg cggcgcaatt
    21961 gcaaaaacag gtacacattc aataccgccc cgatcaaggt gaagagaccg agcgtgattt
    22021 tgatccctac gggctgacct attttcaaaa caaatggtat gtaattggct tttgtcactt
    22081 gcgcgaaagt ctgcgttcat ttcggctgga tcgtatcagc ggaatcaaaa ctctcgaagc
    22141 aggattcagc cgtcccgatg ggtttgatgc gttgggtcac atcatgcagg cgattgccac
    22201 cctgccgcga aaatatccat ttgagatcgt gctaaaaaca gatattgaaa ccgcacagcg
    22261 agaggtgttc gatgtgctgg gtgtgttaga ggcaggccgc gatggtatca tcctgcgcgg
    22321 tagtgtggat gatctcgatt ggctcgccag gcaactatcc attttttcct ttggcttttc
    22381 cgtgcgtaca cccaaagaat tgaaaacagc cttgaagcaa cacgccgtca ggctgggaaa
    22441 tgtgatctga ttctctacat aacataatag caaagtcaaa gtaaggtctt ttggcttata
    22501 atgcctacga atttttcttt gccttaaagt tcagcacagg cttcagccgc cgttttcggc
    22561 ggtgttttat gaataagaac agcctgtctg gtagggcaaa gagacgaatt ttacggagac
    22621 caacttgacc gacgcgcctt ccccattaat ctttataagt tacagccgag tagactcgaa
    22681 agcccaacag cgcctggcag atgccttgcg caagcatggg ctgacagtgt gggtggataa
    22741 cgaaaaactt actccaggaa accctgcatg ggagcgggag atcgagaaag cgatcctgtc
    22801 ttgcagtgct gtaattgcga ttctttcacc tgaagcaaaa cggtcggagt gggtgcgccg
    22861 tgagatcagc tttgccgagc agcataacaa acgcattttc cctgtcttgc tgcgcggcga
    22921 tgaaagttcc accatgacat tgcgtatgat caaccatcaa ttcgttgatc tgcgccaaaa
    22981 tgaaaaagaa ggggtcgagg ttctcttcaa tacgatttca gaatatctgg aatatgtgaa
    23041 ggccaaccct tctggtgaag gcgcgggctc gaattccctg ttctcaaaac caaaggcatg
    23101 gatttttgcc gtactcggca tctttgccct gcttatgaca acttcattcc tcctgaagat
    23161 ggggcccttt accgttccag caacgccaac agccacaaca cccccaacgg ccaccgccac
    23221 catcccgccc actgccacag cccagcctga ggtggtcatc cctgagttct tcacagtgga
    23281 ttttgacacc gatctggaga actgggatac ctttctcgtc ggcgaccctg agaacgaagt
    23341 aaagacaagc gttgaaaaaa gctatatgaa attcgaactc agcggcaaaa atcaacacta
    23401 ctatacctat tacacgctca gaacttataa caatgtccgc gttgatatcc gcagcgaaaa
    23461 ccgcggtgaa tacaacagcg ttgccactgt gatctgccgc tatcatattg acaaggactg
    23521 gtacgaattt aatttcgaca gcggcggtct gtatcagatt ttgtacgtca aatggaacga
    23581 cgataagaca aagaccattt ccacattgat cgccaatggc ggttcgagcc ttattaatgc
    23641 agggaagaat gtcaacgatt attcggtgat ttgcaacgac cgcactcttt cgctgtggat
    23701 caatggcaac gagatcagca ctgtgattga taacaggttt gtgttggagg aaggctatgt
    23761 ggggatcggt gtttcttctg gcacgctgat tccaattgag aacagaattg atacgctcac
    23821 catcagccag ccttaatctg tgtccccttc aaggagccgc ctgtcataaa ggcggctctt
    23881 tgtttttaag cgtttcgagc agaactttcc cgatgccgta taattcagaa ccaaccctat
    23941 ttcttataag gataattcga tggattctct ttcagagcgc cgaaaggcga ttctttttct
    24001 tgcacttgcc gccattttgt ggagcacaag cgggttgttc atcaaaattt tggattggca
    24061 gcccctttcg atcctggcag cccgcagttt ctttgccgcc attgtttttc tcgtttacct
    24121 gcgccgcatc ccgatgcgtt tcaatctttg gcagctgctg gcggcaggga tgttcattct
    24181 gacacagttc ctgttcatca cttccaccaa attgaccacg gctgcaaacg ccatctttct
    24241 gcaatacacg gcgcccattt atgtcgtgtt gcttgccttc tggttcctgc acgaaaaacc
    24301 ctcccgtgtg gattggcttt cgatgcttat catctttatt gggctgaccc tcttctttgg
    24361 tgacaagctc agcacagagg gattctacgg caacctgctt gccatcctca gcggagtgac
    24421 ctcggcggtg atgatggttt ccttccgtgc tcaaaaagat ggcaaccccg ctgagagcaa
    24481 cctcattgca tttttgatca cggctgcatt tggctttccc ttcgtgctaa aagaaacatg
    24541 gaccgtggaa agctggtcca tccttgcttt tcttggcgta tttcaaattg ggtttgcctt
    24601 catatttttt acacagggca tcaaacatat ccctgccctg gaggcaaacc tgatcggtac
    24661 tctggagcca gttttgaatc ccatttgggt gtttctgttt tatggagaga gcatgggcgc
    24721 ttatgctttg atcggcgggt tggtggtatt gggcggggtt gttttgagcg cggttgggag
    24781 tgcaaaggca gtgaaagaca ggcattgaaa tcatgcaacc ttataccttt gaccattctc
    24841 ttggtaattt gctgcacgcc caattgtgat aaatctccgc tcgaattcac cttatgttat
    24901 agtatggaaa atattagttt ggctgaatga aaaggcaaat tatgaccagt aatcacactc
    24961 gtaaccccgg cacgcccctt gaccttgatt gggtgatggg cgcgcacatc aacaaaagcg
    25021 cggtggagcg acgcactgcc acgatcacag gtcgccgaac cgtgaaaaag gattggcagg
    25081 cggcatggct tttacgcgcc gtcacctgca ttgacctgac caccctcgca ggcgatgata
    25141 cgcccggacg tgtaaatcgt ttgtgcgcca aggcgaaaca gccgctacgc cccgatatga
    25201 tggaaaaact tggactcaac ggcgagagaa tcaccgtcgg tgcggtgtgt gtatatccca
    25261 accgtgttgc ggatgcagtg ctggcgcttc aaggctctac cattccagtt gcctctgtcg
    25321 cgacaggttt tcctgcgg
//
LOCUS       DAOQJC010000053        25223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_865_length_25223_cov_9.586499, whole genome shotgun sequence.
ACCESSION   DAOQJC010000053 DAOQJC010000000
VERSION     DAOQJC010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 25223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25223
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_865_length_25223_cov_9.586499"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLE39_09810"
     CDS             complement(<1..418)
                     /locus_tag="PLE39_09810"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68665.1"
                     /translation="MSEITKPPLYWIADYLTPDDVLQHAIHKVILHKHTAFQEMWIVD
                     CGTYGKGLVLDGIWQSSTGDDFLYHESLVQPACVNHGNPKRGLVLGGGEGATIRELLK
                     WKAMERVVMVDLDGEVVEACKVHLPEMHQGAFDDPRV"
     gene            complement(434..817)
                     /gene="speD"
                     /locus_tag="PLE39_09815"
     CDS             complement(434..817)
                     /gene="speD"
                     /locus_tag="PLE39_09815"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNE68666.1"
                     /translation="MNATLKLGEHFIFDLSDCNHEILMDSERAYSLFAQAVRESGLTV
                     VDEGFYKFSPHGFTCFLLLAESHASLHAWPEHNYCAIDLFTCAIGKDMMPLLMKIKQA
                     FGADDFSMRKLDREASVETRLPIAV"
     gene            1174..2325
                     /locus_tag="PLE39_09820"
     CDS             1174..2325
                     /locus_tag="PLE39_09820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE68667.1"
                     /translation="MSFAYLLIEFLDELTYGISEAAWPFIRDDLHLNYVQIGLALSLP
                     GILGNIIEPFIFTFADAWKRRVVLLTGGIFFIISLALTGISTSFLFLLSSYILFNPSS
                     GALVGLSQASLMDLQPERREQNMARWTFAGSLGVVIGPILLSGAAYIGFGWRGMFLGL
                     ACIAILILLFVWKRIPTDQTTTEEIPGFRAILAQFKSTFAALKNWGIVRWLILLEFSD
                     LMLDVLYGFLPLYFVDVAGFSPAQAALSVVVWTGVGMLGDFLLIPLVERIKGIDYLRI
                     SVIIELILFPLFLLSSQAWLKLTIVGMMGFFNAGWYAILKANLFESMHGQSGAALVLD
                     NVSGLFGKLIPFGIGLAAQTFGIGSAMWILIAGPLALFIGLPRKHTSRA"
     gene            2322..3545
                     /gene="hemW"
                     /locus_tag="PLE39_09825"
     CDS             2322..3545
                     /gene="hemW"
                     /locus_tag="PLE39_09825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNE68668.1"
                     /translation="MTTDTSIYIHIPFCKHRCAYCDFNTYAGQEDSIPAYVDALIREI
                     GFIGDQLVKLPTFNVPTVFFGGGTPSLLSAPQFDSILKALTSAFTFTADAEVSIEANP
                     GTISPAKLDAIRKAGINRISFGVQSTNTEELRMLERIHDFFTVIEAVSTARKAGFDNL
                     NLDLIYGLPEQTLTSWQTTLERIVELQPEHISAYALTLEHGTPFGRWASKGLLPIPDP
                     DLAAEMYEFTEDFLESHGYAHYEISNWARKKEVNSSFNIHHSAFVCRHNLQYWRSLPY
                     LAFGAGAHGYANGYRYSNVLRIKTYIERLANYQAPTSNYQFPLSPATVNHKKQSVQED
                     ISDYMINNLRLVNAGVAESDFRLRFGSGLLDVYPKEVEKLLRNDLMEHVKESDIYRLT
                     KRGRLLGNQVFMHFV"
     gene            complement(3589..6432)
                     /gene="ppdK"
                     /locus_tag="PLE39_09830"
     CDS             complement(3589..6432)
                     /gene="ppdK"
                     /locus_tag="PLE39_09830"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNE68669.1"
                     /translation="MTKWVYLFNEVKEVEKRVGGSWDDVKALVGGKGAGLLDMTRADV
                     PVPPFFTVTTEACNAYQKAGKFPAGMWDQELKALKAIESKTGKKFGDPKNPLLVSCRS
                     GAKFSMPGMMNTILNIGLTDTVTEGMIKATGNARFAWDSYRRLVEMFGTVVMDVEDEA
                     FEHPLAEFKNKKGYKLDTEMSADDWKEVVGVYKQVYKKETGFEFPQDPYKQLSLATEA
                     VFKSWNGKRAIAYRRKEGISDALGTAVNICTMVFGNMGDDSATGVAFTRNPSTGEKVM
                     MGEYLLNAQGEDVVAGIRNTDAIQNLIKYMPAAYKQFMGITAKLEKHYKDIQDVEFTI
                     ERGKLWMLQTRNGKRTAKAAVKIAVDMANEGLITKDEAVLRVTPDNVDALLHPQFDDA
                     AMNQAEKEGKYLSKGVNASPGAAVGQVYFDADMAEKMSKEEKQDTIMVRPFTKPDDVH
                     GMIASKGVLTSEGGATSHAAVVARQFGIPCVVGASAIKIDLEKRVMAVGDITVKEGDW
                     ISVDGTTGKVFVGKIPTSEPSLEEQKELMTLLKWADEIAARKDVRQSVAAGYPTRGLQ
                     VWANADYPKDARRARSYGAVGIGLCRTEHMFFEPERLPIVQRMILAETSDARTAALNL
                     LLPHQRKDFDGLFEAMDGYPVIIRLIDPPLHEFMPDEEKLMEEVITMRVKGEHEGLEE
                     KEKLLAAIKGMHESNPMMGLRGVRLSISMPEIVEMQVRAIFEAAADCTLRGIKVKPEI
                     MIPLTGTVKELEWIQPRLERIGKAVMQEKKIKFAYKFGTMIEIPRAAVTADHIATQAQ
                     FFSFGTNDLTQMTFGYSRDDAERNFLITYVQEGILVKNPFQTLDRNGVGKLMQRAILE
                     GRATRPELEVGICGEHGGDPDSIEWCHIIGNNYVSCSPFRVPVARLAAAHAALKHMPA
                     KKAAARKPAAKKAVAKKVVAKKVVKKAVKKGKK"
     gene            6667..7095
                     /locus_tag="PLE39_09835"
     CDS             6667..7095
                     /locus_tag="PLE39_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE68670.1"
                     /translation="MTTKILVIDDDVAITELMNMLLKTHGFEVFTTNSGVEGVKLVEE
                     HRPNVVLLDLMMPDMDGWQVCKAVRSFNNVPILILSAINDPSMVASVLDAGADDFLVK
                     PVPSSVLVAHIRKMVRRNNTGTLSMGMGKPNARTTHPLPS"
     gene            complement(7180..7953)
                     /locus_tag="PLE39_09840"
     CDS             complement(7180..7953)
                     /locus_tag="PLE39_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE68671.1"
                     /translation="MKNISVNGIKLAYDRRGKGAPLVLLHGFPLDHTSWNEVASILED
                     HFDVILPDLRGFGESTTVNTPFSISDMADDIAGLLDSLGIEQVAIAGHSMGGYVALSF
                     LKNYPKRVSGLGLISSQAAADTPERKDGRYKTAVEVAEKGVSVVADAMSPKLSADDRV
                     RVFVRESILRQSQAALIGALKAMATREDMTSFLASIEGQYPVVLLHGNDDVLVPIERS
                     KEIADLLKSSRLFEIPGAGHMPMMEFAKETALALDFLRP"
     gene            complement(7961..8497)
                     /locus_tag="PLE39_09845"
     CDS             complement(7961..8497)
                     /locus_tag="PLE39_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNE68672.1"
                     /translation="MKLVYFDLESQNLFEDVGGRDNIAKLRVACGVTWSTQTNDFSVY
                     WEKDVPALIDELRSATKVIGFNLKGFDYLVLQPYAPQIRFASIPTVDMLLDLQKTLGF
                     RIGLDSVASATLNAAKTADGVQAVEWFRAGELDKVAEYCKADVDITRRVFEFGRDNGH
                     VFYKSKLGSKLKVAVQWK"
     gene            complement(8494..10191)
                     /locus_tag="PLE39_09850"
     CDS             complement(8494..10191)
                     /locus_tag="PLE39_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNE68673.1"
                     /translation="MDNESSDSVQRPSPYAGRWVARVHGRVVAQGGTPEQALRASQES
                     RHKEKPEIVFMSLPLALPPLLEKLLTILPADQEIYLVGGAVRDLLLARVSPDLDFVLP
                     SNGIALARRVAKTLGADFLPLDEERDTGRVIVTNEDGTRTFLDFSIYRSAESTSVFGH
                     SLEEDLRARDFTMNAIAYNLHTGTITDPLNGGTDLRAKLIRACSPTSLSDDPVRILRA
                     IRQAALFGFHIDKQTREWMKESVGQLERISAERLRDEVFKILQGPRPDTAIRALEMLG
                     VFPHLMPELTALKGVQQSAPHVHDVWTHTLSVLGALDQIIASLRVGYDAEQTNDMFTG
                     LLTLRIGRYREQMAKHFSTSLNIDRSQRALLFFAALYHDVCKPQTKTVDENGRIRFFD
                     HEVQGADVVANRAHAFNLSNDEVDRLQAIVRHHMRIHAFASRLEHEKQTPSRKAIYRF
                     FRDAGEAGIDLILLGLADLRGTRAHELTLETWTAYLDVARLLLENYWERPEEVISPPR
                     LVGGHDLIHELNLQPGPLVGQLLELIRENQAAGRIETREQALAFAREELEKGMTDSP"
     gene            complement(10214..10771)
                     /gene="hpt"
                     /locus_tag="PLE39_09855"
     CDS             complement(10214..10771)
                     /gene="hpt"
                     /locus_tag="PLE39_09855"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNE68674.1"
                     /translation="MQNYKDVLAEVLIDAETLQARVRELGAQISADYKNDDLLLICIL
                     RGGVPFTVDLSRCITTPHMVDFMAVSSYGVGKRESSGSARVTLDLQTDIRSKNVLLVE
                     DIVDSGHTISSVLQMLETRQPKSLKVCALLDKPERREAHVPIHYLGFSIPNKFVFGYG
                     LDLDEYYRNLTFVGVVDLEKYHPNE"
     gene            complement(10805..11374)
                     /locus_tag="PLE39_09860"
     CDS             complement(10805..11374)
                     /locus_tag="PLE39_09860"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNE68675.1"
                     /translation="MKHTHGSIEVICGSMFSGKTDELIRRLVRATIAKQKVQVFKPAI
                     DVRYAVEKVASHAGSTFDAIPVEKAAEIRLKLDKDTTVVGIDEAQFFDPEVVEIAREL
                     AARDVRVLVAGLDTDFRGEPFGSMPVLMSEAEDVAKLHAICMVCGENASRTQRLVNGK
                     PARYDDPVVIVGASELYEARCRRHHEVPR"
     gene            complement(11508..12044)
                     /gene="rpmE"
                     /locus_tag="PLE39_09865"
     CDS             complement(11508..12044)
                     /gene="rpmE"
                     /locus_tag="PLE39_09865"
                     /inference="COORDINATES: protein motif:HMM:NF000612.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNE68676.1"
                     /translation="MKADIHPTAYEAKVTCASCGKTWVTLSTKKELRIDVCSNCHPFF
                     TGESARLIDIEGQVDRFYKKLSARQTYVEQQKAKEETANSPERSVDDLELTPRATDAL
                     KKVGILNIGQLLAKLGEGNAAVLEISGFGQSSLTAAKKKLRALGFEVPEVPDAPKPPK
                     APKTAEASEPSAEAGAAE"
     gene            complement(12046..12306)
                     /gene="rpmA"
                     /locus_tag="PLE39_09870"
     CDS             complement(12046..12306)
                     /gene="rpmA"
                     /locus_tag="PLE39_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNE68677.1"
                     /translation="MAHKVGGGSSRNGRDSNAQRLGVKRYAGQFVLSGNILVRQRGTK
                     IKPGLNVLVGKDDTLFAVADGIVMFDVFHGRKRVNIIPVEAE"
     gene            complement(12334..12987)
                     /gene="rplU"
                     /locus_tag="PLE39_09875"
     CDS             complement(12334..12987)
                     /gene="rplU"
                     /locus_tag="PLE39_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNE68678.1"
                     /translation="MRIAIVESGGKQYRAVEGTTIDVDRLAYEVGHKFDFERVLLMAD
                     GDVTMVGTPTVGDIKVSATVVDHVKGPKVISFKYRPKKRIRVKGGHRHFYTRLMIDFI
                     GKAGEDRKPVAAKAVESEKKAASGADDLTKIEGVGPKVAKVLNEAGISSFADLAGAKA
                     ADVQKALDAAGLQMMDPKGWISQAKLAAKGDWEGFEKLQGELKGGRKAKSADKKKEK"
     gene            complement(13222..13695)
                     /locus_tag="PLE39_09880"
     CDS             complement(13222..13695)
                     /locus_tag="PLE39_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68679.1"
                     /translation="MPVPTRVPAKSGVIAAKTSSGSIIKFSDQLTKSVSDVAQIIEQH
                     KGMIDSIQEIALELTTSIGTLHTLTVKYARTANGILDILLPILRNLPIIPKNVMQMLV
                     TLEATTQKIIDNEVSTSKTITDVQSGLRTGDVNKIKGHAGQLQNVTKSLTAMLPK"
     gene            13855..14526
                     /locus_tag="PLE39_09885"
     CDS             13855..14526
                     /locus_tag="PLE39_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNE68680.1"
                     /translation="MNQMIQLGSVNGYLAKPGGTGPFPAMIVIQEWWGLDAQTKSIAD
                     RFAQEGYLAFAPDLYHGELAQLGDGDTAMKLVQKYGPVAHNDLTALYDALKSHPDCNG
                     KIGSVGFCFGGRMSLSLSTLRPVNAVCTFYGGGMQMIFDQLRANLKAPVLGFFGDADV
                     SIPAGTIEEFDKLLDEVGVEHEVITYPNSGHAFFRDSDPSVYIPEASKDAWERAKKFF
                     SKNLN"
     gene            complement(14591..15610)
                     /locus_tag="PLE39_09890"
     CDS             complement(14591..15610)
                     /locus_tag="PLE39_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68681.1"
                     /translation="MKTLHQRITPVILGLLSFLLFAFVPLSKTHGMVRESAGGKADGP
                     DVYYVYITVLNISNIDLSAGTYAVDFFISFICETPPCQYEPHWDVMNATEIIDPEDQG
                     TSIPFEVYDYRLKTSLIGYVDYTFYPFDYLYVDIIIEDKEYSNDELVYRSAAVEVDPM
                     LFNPSGWYYRPEYDGDMNDIVVYPNDDISYDRLYIWLFMERDRFSGFMKTIFAALVIV
                     LVGMLSFLMKAEAAGERLALVSSTLVAVVLYHISLVSGVPATGYLTFIDKFMIGTYAV
                     VFVSLVISVAMMVYKNNDELGKAEKLHLRTRWIIPLLWVFLMVCVFIVELILPYRQML
                     AANGG"
     gene            15715..17799
                     /locus_tag="PLE39_09895"
     CDS             15715..17799
                     /locus_tag="PLE39_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE68682.1"
                     /translation="MASLNLLQRQIIESPLDSRIFVSGRAGTGKTTTGVERMRFLLEQ
                     GVPADSILMLTPQRTLQEPYLELLYSPERRAGGEVTSATIGGLAKRMCDLFWPIAAEA
                     AGVKNPERAPVFLTLETAQYYMAFIVRPLLEQGYFQSLTIDRNRMYSQIIDTLNKAAV
                     VGFPYTEIGTRLDAAWVGDPGQRRIYQDVQDCAIRFREYCLEHNLLDFSLQLEIFTDI
                     LWPQEQVQNYLKQTYRHLIYDNIEEDAPRAHDIIREWMPDFESALLIYDNDAGYRRFL
                     GGDAASGYALIETCESQIEFSESFVSSENIIQLSNSLVSAVTNQSTGEEAYPPDTMEF
                     IMAHFYPEMLNEVVGKIKTLIEEGVPPAEIVVLAPYLSDALRFSIADRLEKENIPWRS
                     HRPSRSLRDEPASNALLNLSALAHPHWNVRPTKFDMMQTLMFALDTDLVRAQLLTEVV
                     YGQRDLRLSPFEEIRPEMQERITYRLGEKYTTLRNWLLEYREAAPLPLDFFLRKLFGE
                     VISQPEYGFHSNMDAVRVAASLIESIRKFRNAMEPFDQTGFDMGREYLSMLADGVIAA
                     QYLESWRSEDKEAVLVAPAHTFLMMNHPVTIQFWLDPGSVGWVQRLSQPLTHPYVLSR
                     GWDPSPAHFWMDTDEVREETDALAKLVGGLLSRCREKVFLALADLSESGMEQRGSLLR
                     AFQKVLSLQGQE"
     gene            17888..19102
                     /locus_tag="PLE39_09900"
     CDS             17888..19102
                     /locus_tag="PLE39_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68683.1"
                     /translation="MKTRELLWILLLAALTTLGLDLVTNAINTQGNAWDFLYYIALAK
                     DGFHADPLASPFAYRYLTPFIVHGISLLGFSIERGFGIIAYFGAIMQLTGIFFFIKWL
                     GNSTKGAYLAMAVTALSLFNVKFLLFDIYRPDHLAYALILLQTYLAFNRKFLPLLLTT
                     LIASQIREFNIIPMVAYLFMSFRTTERGLFIKQAAISTICLVPAILLPRLLIPVTENY
                     QLVGLSGDALLTTVILPFIPSVDVNFIFSIFAYLLPLLFLADLKTIKAAFASLPENQR
                     GYVLTYTVLVLLLSFFGGTDFNRFATFLFLPQIILIGLITPAISSLQISLMLAVTFIF
                     NRLWMHIPDWDVEKYRDFYGGFSLRLNMSTLNRIMELAAFWALGFGVRKWQGTTQTLE
                     SQRHENTTPQRK"
     gene            19136..21205
                     /locus_tag="PLE39_09905"
     CDS             19136..21205
                     /locus_tag="PLE39_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68684.1"
                     /translation="MSFYSLNYTLQDLIGYTLAFFLFPLVIVLPGYVFSSALDLFDFK
                     RRLPIVKFGIGLLVSFAVSPIIFNLTSSLGSMTFSIIVIGGFVLAFLFFLAKENFSLQ
                     IAPTAKPLVWICIGWVVFVAYSLVDIHWGDQLYVSVVSFDQTSRVSVIDAMTRTGVPP
                     INPSYFPGAPTLLTFLYYFWYILASLVDILGGTLVDARAALNASSAWSGLGLMAAIAF
                     YLRVRNVNLGRAVWRSARIGVSLLAVSGLDVLPTIIIMLGVRRIYGTVEGWNLQMQIP
                     TWNSSILWAPHHIAAMVACFTAILLALSAREKPLRRKMTLMTIAGMAFASSFGLSIWV
                     TFLFGVFWAVWVLALFIQKTDRSLVVPMIFCGVVAILLAGSFILGLLQPQGEGGVTQS
                     TVIFDVRTLYLLEGFVTAWPPLARSLLMLALLPLNYFLELGFFIVAGYFWVRSKNKTE
                     IVSDPFYLVEVLLMSVVLLIGSTLRSTIANNDLGWRVWLPGQFILLIWGVDVIETLFS
                     TKSASDSPKADSVKRKNLALTLLILGVLTSSVDALFLRVTWPLRVGVDEGRRGYSARL
                     AYDFLRDQVPADVITQNNPLPLADRPSGLYGTHQMVISDRTIYGVSLTTYDQFVHDLA
                     FLFTESFSNWQPIDTACQKYSIDLLIFNDLDPAWPSLATLKTQRPALYENEHYSIFAC
                     GSYTKIR"
     gene            21298..22326
                     /locus_tag="PLE39_09910"
     CDS             21298..22326
                     /locus_tag="PLE39_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68685.1"
                     /translation="MKNKQIPTLIGFFILMYACNFGTPASITPTPDRVETQQPATATA
                     EISPTTAPSPSNGPTLANCPMFPADNYWNAPIDTLPTHSQSEAWIDSIGRTVSFHMDF
                     GSGEYDGGLIGIPFNIVTGSAVPKVGFEFDYADESDPGPYPIPENPNQEAGGDRHILV
                     LDTDTCTLYEVFDASFDGGQWRGGSGAIWDLNSNTLRPDTWTSSDAAGLPILPGLVRY
                     DEIAAGEIKHALRFTIEKTAGYIWPARHLTDDPQDGIPPMGARFRLKADYDISGFPQE
                     MQIILQAMKTYGIILADNGSNWYISGAPDDRWDNDMLHLLDVLTGNDFEAVDTSGMMV
                     DVDSGTAK"
     gene            22340..>22591
                     /locus_tag="PLE39_09915"
     CDS             22340..>22591
                     /locus_tag="PLE39_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68686.1"
                     /translation="MSDPYEFIDMAQKAFDFLVKDFGFQVTVTKSQGTYHSEVVFTTH
                     KLKITVGIWGQSNEAWIIFFPQDKNFPPLHFSSLLNRITG"
     assembly_gap    22592..22691
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22692..24860
                     /locus_tag="PLE39_09920"
     CDS             <22692..24860
                     /locus_tag="PLE39_09920"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNE68687.1"
                     /translation="GKTHILSALAAKIIREGDLQDGQEVLIVTLVNSAVDNFDKRIKQ
                     FFNNPIESLYKYRVRTLHGLAHDIVREKPARVGLEERFSIIDEREAGFIRRESVNAWL
                     ASNSLDDYLDPALDNSKQDWIRRSQLPDLVDSLALAFIRSAKDRLLTPESLRAKLDAS
                     FAPLPLAELGYSIYADYQRALAYRGAVDFDDLIRYALTLLESDEEFLERLRYRYPYIL
                     EDEAQDSSQTQERILALLSGENWVRVGDPNQAIFETFTTASPELLRKFIRENPSQDMP
                     ESGRSQPSILAVANHLIDWVLNAHPDPNVRTALSLPYIIPVPKNDPQQNPPENPEGIK
                     FISRKYSPEDELEAVAKSVKNFVASIAEIPDEEKPTIAVLVPRNARGVEVVNELRKRG
                     IEPIELISSTTETRAAAGSLNYLLSYLADPQSARKLSKAYEVWRRDWREQATGNDDSR
                     AGESDPELLKEVTAILRRLVNVENFIAPSQADEWLAAIGEVDSPQVIEELGLFKVNVQ
                     RWLNAAVLPIDQLVLTLAQDVFGKASDLALAYKLALVLRKAADDHADWRLPELASELA
                     VIAKNERRFIGFSDDDSGFDPERHRGKVVVVTMHKAKGLEWDRVYMMSVNNYDFPSNM
                     PNDRFISEKWFVRSGLNLEAEALEQLKALESNSEYDWYEEGAATMRSRLDYVKERLRL
                     LYVGITRAKRDLIVTWNSGRQGDATPSLAMSELMGWWENK"
     gene            complement(24901..>25223)
                     /locus_tag="PLE39_09925"
     CDS             complement(24901..>25223)
                     /locus_tag="PLE39_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68688.1"
                     /translation="PFEGYTTDGVTPIGASFSMTIKLEPIEGGTRVINLWGKARGAWI
                     RRKLNDLLQKLIFVSKNDQGYEKLRQRMQQDLADGTTFVSPPIEVDNAQIAALASQAL
                     TTEH"
BASE COUNT         6077 a         6692 c         6080 g         6274 t
ORIGIN      
        1 cgacacgcgg gtcgtcgaac gcgccctggt gcatttcagg gagatgtacc ttgcaggctt
       61 ccaccacttc cccgtcgagg tccaccatca caacccgttc catggccttc cacttcaaca
      121 actcgcggat ggttgcccct tccccgccgc cgagcacaag tccgcgtttg gggttgccgt
      181 ggttcacgca ggcgggctgc accaaagatt cgtgatacaa aaagtcgtca cctgtggaag
      241 attgccagat gccatccagc acaagtcctt tgccataagt gccgcaatcc acgatccaca
      301 tctcttggaa agcggtgtgc ttatgcagaa ttactttatg gatggcgtgc tgcaaaacgt
      361 catcaggggt caggtaatcg gcgatccaat acaggggcgg ttttgttatt tcggacataa
      421 tggtttcctc tttttatacg gcgatcggca atctggtttc gacagatgct tcgcggtcga
      481 gcttgcgcat cgaaaaatca tccgcgccga aagcctgttt gatcttcatc aataaaggca
      541 tcatatcctt gccgattgcg catgtaaaga ggtcaatggc acaataatta tgttcgggcc
      601 aggcatgcaa acttgcatgc gactctgcga gaagcaagaa acaggtaaag ccgtgcgggc
      661 taaacttgta aaagccctca tccacaaccg tcaggccgct ttcacgcaca gcctgcgcaa
      721 acagcgaata agctcgctcg ctatccatga gaatctcatg gttgcagtcg gagagatcaa
      781 aaataaaatg ctctcccaat ttcaaagtcg cgttcactca cacctccagg ggttaaaaaa
      841 tgaaagaacc cgcccgggaa caccatcttt ttcatttcaa aacgatccgc aagatgtatt
      901 ccttgcgtaa cgagtgaaac tgttccagag cagactctat gtatgggcat ttatactatg
      961 tcacggcgca gtgtcaatat tttttagctg tgaatattct ttatcacatc accaaatcga
     1021 aatcaaaatc gtcaccaaaa gggctgaaag ggtcacaatc gtcattcaat ttgtttttcc
     1081 atgcatgatt ttacacaagc ccaaaacagg tggtaaaaac atggaactgt gttaaatttg
     1141 agggcaaaga caccccacat cactggaaac catatgagct ttgcctatct gctgattgaa
     1201 tttcttgatg aacttaccta tggcatcagt gaagccgcct ggcccttcat ccgcgacgat
     1261 ctgcatttga attatgtcca aatcggtctt gcgctctccc tgccgggcat cctcggcaat
     1321 attattgaac cgttcatttt cacttttgca gatgcatgga aacgccgtgt cgtccttctg
     1381 actggcggaa ttttctttat catttccctt gcactgacgg gaatcagcac ctctttcctg
     1441 ttccttctat cctcctacat tcttttcaac ccgtcctctg gcgcgcttgt tgggttgtca
     1501 caagcctcgc tgatggatct gcaacccgaa cgccgcgaac aaaacatggc tcgctggacc
     1561 tttgcaggct ctctcggtgt ggtcattggc cccattctct tgagcggtgc agcctatatt
     1621 ggttttggct ggcgcggcat gttccttggg ctggcatgca ttgcgattct gatcctgctt
     1681 ttcgtgtgga aacgcattcc caccgaccaa accaccaccg aagaaatacc aggctttagg
     1741 gcgatccttg cacaattcaa atccaccttc gcagcactca aaaattgggg aattgtccgc
     1801 tggctgattt tgcttgaatt ttccgatctc atgctcgatg tgttgtacgg ctttctcccg
     1861 ctttattttg tagatgtggc gggcttttca ccagcccaag ccgcgctctc ggtggtcgta
     1921 tggactgggg ttggcatgct cggagatttt ttactcattc cacttgtgga acgcataaaa
     1981 ggcatcgact atcttcgcat cagtgtaatc atcgagctca ttctcttccc gctgtttctg
     2041 ctttcctcac aagcatggct caaactgacc atcgtcggca tgatgggatt cttcaacgcg
     2101 ggctggtatg ccattctcaa ggcaaatctg ttcgagtcca tgcatggtca aagcggcgcg
     2161 gcgctggtgc tcgacaatgt ttcaggattg tttggtaaac tcattccctt cggcatcggc
     2221 ctcgcagcac aaaccttcgg cattggctcc gccatgtgga ttctgatcgc aggcccgctc
     2281 gccttgttta tcggactccc cagaaagcac acatcccgcg catgaccaca gacacctcta
     2341 tttacattca cattcccttt tgtaaacatc gctgcgccta ctgcgatttc aacacctacg
     2401 caggccagga agattcgatc cccgcttatg tggatgccct gattcgtgaa atcggtttta
     2461 ttggcgatca acttgtaaaa cttccgacat tcaatgtccc aacggtcttt tttggaggag
     2521 gaaccccgtc gcttctttcc gcgccgcaat ttgattccat tctaaaagca ttgacctccg
     2581 ccttcacatt caccgctgac gcggaagtaa gcatcgaagc caaccctggc acaatctccc
     2641 ctgcaaaact ggatgccatc cgcaaagcag gcatcaaccg catcagcttc ggcgtacaat
     2701 cgaccaacac cgaagaactg cgcatgcttg aacgcatcca cgactttttc accgtcatcg
     2761 aagccgtgtc cactgcacgc aaggcggggt ttgacaatct caacctcgat ctcatttacg
     2821 gccttcccga acagactctc acttcatggc agaccacgct cgaacgaatt gtggaattac
     2881 agcccgagca catctccgcc tacgcgctga cccttgaaca cggcacgccg tttgggcgtt
     2941 gggcatcgaa aggcctgctc cccatccctg accccgatct tgccgccgaa atgtatgaat
     3001 ttacagaaga ttttttggaa agccacggat atgcacatta tgaaatttcg aattgggcaa
     3061 ggaagaaaga agtaaattca tcattcaaca ttcatcattc tgcatttgtt tgccgtcaca
     3121 acctccaata ctggcgctcg ctcccctacc tcgccttcgg cgcaggtgcg catggatacg
     3181 ccaatggtta tcgttactca aatgttctcc gaatcaaaac atatattgag cgtctcgcca
     3241 attaccaggc accgacctct aattatcaat ttcccctctc acctgccaca gtcaaccaca
     3301 agaaacaatc ggtgcaggaa gatatttcag attatatgat caacaacctc aggctggtga
     3361 atgccggtgt cgctgaatca gatttcaggt taaggttcgg aagcgggctt ttggatgtct
     3421 atccaaagga agtggagaaa cttcttcgca acgatctgat ggagcatgta aaagagtccg
     3481 atatttacag gctcaccaaa cgcggcaggc tattggggaa tcaggtcttc atgcattttg
     3541 tgtaacaaaa aatccccgcc atcgaaaaag atggcgggga ttttcaaatt acttcttacc
     3601 cttctttaca gccttcttca caactttttt cgcgacaact ttctttgcaa ctgctttctt
     3661 tgcagcgggc ttcctggcag cagccttttt ggcgggcatg tgcttcaatg cagcatgagc
     3721 ggcagccagg cgggcaacgg gcacgcggaa gggcgagcag gaaacatagt tgttaccgat
     3781 gatgtgacac cattcgatcg agtcagggtc accgccgtgt tcaccgcaga tacccacttc
     3841 gagttcgggg cgggtggcgc ggccttcaag aatggcgcgc tgcatcagtt tgcctacgcc
     3901 gttacggtcg agggtttgga acggattctt gacgaggatg ccttcctgaa cataggtgat
     3961 gaggaagtta cgttcggcat cgtcacggga ataaccgaag gtcatttggg tgaggtcgtt
     4021 ggtgccgaag gagaagaact gtgcctgtgt ggcgatatgg tcagcggtca ctgcggcgcg
     4081 cggaatttcg atcatggtgc cgaatttata ggcgaatttg atcttctttt cctgcatcac
     4141 ggccttgcca atgcgttcga ggcgcggctg gatccattca agttctttca cggtgccagt
     4201 gagcgggatc atgatctcag gctttacttt gattccgcgc agggtgcaat cggcagcggc
     4261 ttcgaagatg gcgcgaacct gcatctcgac gatctcaggc atggagatgc tcaagcggac
     4321 accgcgcaag cccatcatcg ggttggattc gtgcatgcct ttgatggctg cgagtagttt
     4381 ttctttttct tccagccctt catgttcgcc cttcacacgc atggtgatga cttcttccat
     4441 gagtttttct tcatcaggca tgaactcatg cagcggcggg tcaatgaggc ggatgatgac
     4501 agggtagcca tccatcgctt caaagagacc gtcaaagtcc ttgcgttgat gcgggagcaa
     4561 caggttgagg gcggcagtac gggcgtcact tgtctcagcc aggatcatgc gctgtacgat
     4621 cggcaggcgc tcaggctcaa agaacatgtg ctcggtgcgg cacaagccga taccaacagc
     4681 accgtacgag cgggcgcggc gggcatcctt cggataatcg gcattggccc aaacctgcaa
     4741 accgcgggtc gggtaaccag ccgcaacaga ctggcggaca tctttgcggg cggcgatttc
     4801 atcagcccat ttgagcaggg tcatcagttc tttctgctct tcgagagaag gttcagaggt
     4861 tgggatcttt ccgacaaaca ccttgccagt ggttccatcc actgaaatcc aatccccttc
     4921 ttttacggtg atatcgccaa cagccatgac acgtttttca aggtcgatct tgatggcaga
     4981 agcgcccact acgcagggga taccaaattg gcgggccacc actgcagcat gagatgtcgc
     5041 cccaccttca ctggtcaaca cacctttgga tgcgatcatg ccgtggacat catcgggttt
     5101 ggtgaacgga cgcaccatga tggtgtcctg tttctcttcc ttcgacatct tctcagccat
     5161 atccgcatca aaatagacct gtccaactgc tgcaccagga gaggcattca cgccctttga
     5221 gaggtactta ccttccttct cagcttgatt catggccgca tcatcaaatt gcgggtggag
     5281 caatgcatcc acgttatcag gagtgacgcg cagaacagct tcgtcttttg tgatcaagcc
     5341 ttcgttcgcc atatccacgg cgatcttcac agcagctttt gcggtgcgct tgccattacg
     5401 ggtttggagc atccacaatt tgccgcgttc aatggtgaat tcgacgtcct gaatatcctt
     5461 gtagtgcttt tcgagcttgg cagtaatgcc catgaactgc ttataagccg cgggcatgta
     5521 tttgatgagg ttctggatcg catccgtatt gcggatacct gccaccacgt cttcaccctg
     5581 ggcattgagc agatactcgc ccatcatcac tttttcgcca gtggatgggt tgcgggtgaa
     5641 tgccacgccg gtggcagagt catcacccat attgccgaat accatcgtac aaatattaac
     5701 ggcggtgccg agcgcatccg agatgccttc cttgcggcga taagcaatgg cacgttttcc
     5761 gttccatgat ttgaatacag cctcggttgc cagtgatagt tgtttatagg gatcctgcgg
     5821 gaattcaaaa ccagtttctt tcttgtagac ctgcttgtat acccccacca cctctttcca
     5881 atcgtccgcg ctcatctcgg tatcgagctt gtagcccttc ttattcttga actcagccaa
     5941 cgggtgctca aaggcttcgt cttccacatc cattaccact gtgccgaaca tttcaaccag
     6001 gcggcggtac gagtcccatg cgaagcgggc attgcctgtc gctttgatca tgccttcggt
     6061 aaccgtatca gtcaacccga tgttgaggat cgtgttcatc atccccggca tggaaaactt
     6121 tgcgccagat cggcaggata ccaaaagcgg gttcttcgga tcaccaaatt tcttgccagt
     6181 cttgctttct atggccttga gagctttgag ttcctggtcc cacatacccg caggaaattt
     6241 tccagctttt tgataagcat tacatgcttc cgtcgttacg gtaaagaagg gaggaaccgg
     6301 cacatctgca cgagtcatat ccaacagacc tgcacctttg ccaccgacaa gagccttgac
     6361 atcatcccat gatccgccaa cgcgcttctc aacttctttt acctcgttaa aaagataaac
     6421 ccatttggtc atttcagcct cctaaaagga atagattttc aaattcattt gtgaaatatt
     6481 ttaccaaaaa cactctaatt cacacatgac aaaaatcacc agatactctg atatcaatct
     6541 gtaaacccag gaggcaaaat cccatatttt ccattattaa aatgcaatga acattgcaaa
     6601 attctgttaa taccctgcaa agtgattccg ggggcaaatc agggataata tggcaaggaa
     6661 aaaagtatga caactaagat actcgtaatt gatgatgacg ttgcaatcac tgagttgatg
     6721 aacatgctgt tgaaaactca tggatttgaa gttttcacca ccaacagcgg tgtggaaggt
     6781 gtgaagctgg ttgaagaaca tcgtcccaac gtcgttctac tcgacttaat gatgccagac
     6841 atggatggat ggcaggtttg caaagcggta cgctccttca acaatgtccc catcctcatc
     6901 ctttctgcga tcaatgatcc cagcatggtg gccagcgttt tagatgcagg cgcagatgat
     6961 tttctggtta agccagtgcc gagcagcgtc cttgttgccc acatccgaaa gatggtcagg
     7021 cgcaacaata cgggaacgtt aagcatggga atgggtaaac caaacgcccg caccacccat
     7081 ccgcttccat cctgacatat ctctttggat cttgcatata taaagcccat cctttttatt
     7141 caaaggatgg gctttatggt ttttacatgg gagaaagact cacggcctca aaaaatcaag
     7201 cgccaacgca gtctcttttg caaattccat cattggcatg tgaccagcac cggggatttc
     7261 gaacaaccgt gacgacttca aaagatccgc tatttctttt gacctttcaa tggggaccag
     7321 cacatcgtca tttccgtgga gcaataccac agggtattgt ccttcaatgg aggcaagaaa
     7381 agatgtcata tcttcccgtg ttgccattgc ttttaatgca ccaattaagg cagcctggct
     7441 ctgccgaaga atagactcac ggacaaacac ccgtacccga tcatcggcag ataactttgg
     7501 agacattgca tcggcgacca cgctcacccc tttttcagcg acctctacag cggttttgta
     7561 acgaccgtct tttctttccg gtgtatctgc tgcagcctgc gaagagatca gccctaaacc
     7621 actcacccgt tttggataat ttttcaggaa agacagcgcc acatatccac ccatggaatg
     7681 accagcgatc gcaacctgct ctatgcccaa agaatccaac agaccggcaa tatcatctgc
     7741 catatctgag attgaaaatg gggtgttcac ggttgtggat tcgccaaatc cgcgcagatc
     7801 ggggaggatg acatcgaaat ggtcttccaa tatggaggca acttcgttcc aactggtgtg
     7861 gtcaagggga aaaccgtgaa gaaggacaag gggagccccc tttccacgac ggtcatatgc
     7921 caatttaatt ccattgactg aaatattttt catgcctctc tcacttccac tgtacggcaa
     7981 ctttcaactt actccccagt tttgacttgt aaaaaacatg gccattatca cggccaaatt
     8041 caaaaacgcg ccgtgtgata tccacatcgg ctttgcagta ttctgctact ttatccaact
     8101 ctccagcacg gaaccactcc accgcttgaa cgccatcggc ggttttggca gcattcaggg
     8161 tggcactggc aaccgaatcc aagccaatgc gaaagccgag agttttttgc agatccagga
     8221 gcatatccac ggttggaatg gatgcgaagc gaatttgcgg agcgtagggc tggagcacaa
     8281 gataatcaaa cccctttaag ttaaatccga tcacctttgt tgcagacctg agctcatcga
     8341 tcaatgcagg gacatctttt tcccaataca ctgaaaaatc atttgtttgt gttgaccatg
     8401 taaccccgca tgccacgcga agtttagcga tgttatcgcg cccgccaaca tcttcaaaaa
     8461 ggttttgtga ttccagatca aaataaacga gtttcatggc gaatctgtca ttcctttttc
     8521 caattcttcg cgggcaaagg ctagggcctg ttcacgcgtt tcgatcctgc cagcggcctg
     8581 gttttcacga atcaattcca aaagttgccc caccaatggc ccgggctgaa gattcagttc
     8641 atggatcaaa tcgtgaccac ccacaaggcg cggcggggaa atcacttctt ccgggcgttc
     8701 ccagtaattc tcaagcagga gacgggcaac atcaagatag gcggtccatg tttcgagggt
     8761 aagttcgtgg gcacgtgtgc cacgcaaatc ggccagccct aaaagaatga gatctatgcc
     8821 cgcttcaccc gcatctctga agaagcgata aatggcttta cgtgacggcg tttgtttttc
     8881 atgctccaat cggctggcaa atgcatggat gcgcatatga tgcctcacga tggcttgcaa
     8941 gcgatcgact tcgtcattac tcaggttaaa agcatgagcg cgatttgcca cgacgtccgc
     9001 accttgaact tcatggtcaa agaaacggat acggccattc tcatccacag ttttggtttg
     9061 cggcttgcat acatcatgat acaacgctgc aaagaataga agcgcgcgct gggaacggtc
     9121 aatattaagt gaggtggaaa aatgctttgc catttgctca cgatatcgcc cgatccgcaa
     9181 agtcagcaga cctgtgaaca tgtcgttggt ttgttcggcg tcataaccaa ctcgcaagga
     9241 ggcaatgatc tggtcaagcg caccaagcac ggataatgta tgtgtccaaa catcatgcac
     9301 atgcggtgca gattgttgaa ctccctttaa ggccgtcagc tcaggcatga ggtgtggaaa
     9361 gacacccagc atttccaggg cgcggatggc tgtatccggt ctggggcctt gcagaatttt
     9421 gaatacctca tcacgcaagc gttcggccga aatacgctcc agttggccga cagattcttt
     9481 catccattcg cgggtctgtt tatcaatgtg gaatccaaaa agggcggcct gacgaatcgc
     9541 acgcaaaatg cggacggggt catcagacag ggaggttggc gaacatgcac ggatcaattt
     9601 tgcccgcaag tcggtaccgc cattcaaggg gtcggtgatg gtgcctgtat gtaaattgta
     9661 ggcgatggcg ttcatggtga aatcgcgggc acgcaagtct tcttcgaggc tatgaccaaa
     9721 aacggaagtg gattctgcgc tgcgataaat ggagaaatca agaaaagtac gggtgccatc
     9781 ttcattggtg acgatcacac gccccgtgtc acgctcctcg tcgagaggca aaaagtcagc
     9841 gccgagggtt ttggcaactc ttcgagcaag agcaatgcca ttcgatggca gaacaaaatc
     9901 aaggtcaggt gagacgcgag ccagtaaaag gtcacgcact gccccgccaa cgaggtaaat
     9961 ctcctggtcg gcaggcagga tggtcagcaa tttttccaat aacgggggaa gtgcaagtgg
    10021 aagtgacata aaaacaatct ctggtttttc cttgtgacgg ctttcctgtg aggcacgcaa
    10081 tgcctgttct ggcgtgccgc cttgagccac aacccttcca tgcacacgag caacccagcg
    10141 accggcatat ggtgaaggtc tttgcacaga gtcagaagat tcgttgtcca ttttcagccg
    10201 ttggtggcag ggattattcg ttggggtgat atttttccag atctactaca ccaacaaagg
    10261 tcaggttgcg atagtattca tcgaggtcaa ggccataacc aaatacaaat ttgttcggga
    10321 tcgagaagcc aaggtaatga atgggaacat gggcctcgcg gcgctcaggt ttatcaagca
    10381 gtgcgcaaac tttcaatgat tttggctggc gggtttcaag catttgcaaa accgaagaga
    10441 tggtatggcc gctatctaca atatcttcaa ccagcaggac attcttggaa cgaatatctg
    10501 tttgcaggtc aagggtgacg cgggctgagc cgcttgattc gcgctttccc accccatagg
    10561 atgagactgc cataaaatca accatgtgcg gagtggtgat gcaacggctg agatccacgg
    10621 taaagggcac ccccccgcgc agaatgcaaa tgagcaggag gtcatcgttt ttgtaatcag
    10681 cactgatctg cgcgcccaat tctcttacac gtgcctgcaa ggtctccgcg tcaatcaata
    10741 cttcggcaag aacgtctttg taattttgca tgggattatc ctggttgtgt tcgagagttt
    10801 tgacctagcg tggaacttca tgatgcctgc ggcagcgtgc ctcatacagt tcggaagcgc
    10861 caacgatcac aacggggtca tcataacggg cgggcttgcc attgaccagt ctttgtgtac
    10921 gtgaggcatt ttctccacag accatgcaga tggcatgaag ttttgcaaca tcctcagctt
    10981 cagacatcaa tacaggcatg gaaccaaacg gctcaccgcg aaaatcggta tccagcccag
    11041 ccaccagtac acgcacatct cgtgccgcca attcgcgggc aatttcaacc acttcagggt
    11101 cgaagaattg agcctcgtca ataccaacga ccgttgtatc cttgtccagc ttcaaacgaa
    11161 tctctgctgc tttttcaacg ggaattgcat cgaaggttga gcctgcatga gatgccacct
    11221 tctcaacggc atatcgaacg tcaatggctg gcttgaacac ctgcactttt tgttttgcaa
    11281 tggtggcacg caccagacgg cgaatcaatt catcggtctt tccgctgaac attgacccgc
    11341 aaatgacttc gattgaacca tgcgtatgct tcattcagat tctcccaatt ttttataaat
    11401 taaccaacct gaaaaaatat tttacaaaaa caggcagatg cttaagcatc tgcctgtcta
    11461 gtttacctga gaaacctata cctgacaaag tacccgaaag ggaaatccta ttcggctgcc
    11521 cctgcttcgg cggaaggttc gctggcttca gcggtcttcg gggctttggg aggcttgggc
    11581 gcatcgggaa cctcggggac ttcaaagccc aaggcacgca acttcttctt ggcggcggtg
    11641 agggaagact gaccaaagcc agagatttcc aacacagcgg cattgccttc accaagtttg
    11701 gccaacaact ggccgatatt caaaataccg accttcttga gggcgtcagt ggcacgggga
    11761 gtgagttcca aatcgtccac ggaacgttcg ggagaattgg cggtttcttc cttggccttc
    11821 tgctgttcga cataggtctg gcgtgcggaa agcttcttgt agaagcgatc cacctgacct
    11881 tcaatgtcga tcaagcgcgc agattcaccg gtaaagaacg gatgacaatt ggagcacaca
    11941 tcaatgcgca actctttttt ggtggaaagt gtcacccagg tctttccaca ggaagcacaa
    12001 gtgacctttg cttcgtaagc agtgggatgg atatcagctt tcattctatt ccgcctcgac
    12061 cgggataatg ttgacgcgtt tgcgtccatg gaatacgtcg aacataacaa tcccgtctgc
    12121 aacggcaaac agggtatcgt ccttgccaac caacacattc aagcctggct tgatcttggt
    12181 gccgcgctgg cgaacgagaa tatttccaga gagaacaaat tgaccggcgt aacgcttaac
    12241 gcccaagcgt tgggcattgc tgtcgcgacc gttgcggctt gatccgccgc ctactttatg
    12301 tgccatttct acctcgcttc actaataaca gctttacttt tcttttttct tatcagcgga
    12361 cttggctttg cggccgccct tcagttcgcc ctgaagtttt tcgaaacctt cccagtcgcc
    12421 tttggcggcg agttttgcct gggagatcca gcccttcggg tccatcatct gcaaaccagc
    12481 agcatcaagc gccttttgaa catcggcagc tttggcacca gccagatcgg caaatgagga
    12541 aatgccagct tcattcaaga ccttggccac tttcggacca acaccctcga tcttggtaag
    12601 atcatccgct ccgctagcag ccttcttttc agattcaacg gcttttgcgg caacgggttt
    12661 gcggtcttca ccagccttgc caatgaaatc aatcatcaaa cgggtataga agtggcgatg
    12721 accacctttt acacggatgc gtttcttggg gcgatacttg aaggaaatta ccttggggcc
    12781 cttgacgtga tccacaacag tagcggagac tttgatgtca cccacagttg gggtgccgac
    12841 cattgtcaca tcaccatctg ccatcagcag aacacgttca aagtcaaact tgtggcctac
    12901 ttcgtaggcg aggcgatcca catcaatggt ggtgccttca acggcgcggt attgcttgcc
    12961 gccgctttca acgattgcaa ttctcattat atatttctcc agtcatcact tcgccgcgca
    13021 tcccgcggac cactttaccc gtcaacatac aggggaggtc tacgccgaac cgtggggaag
    13081 ctgggttaaa tttttagcgc gcggtattat acatgcaggg aggcgcacct gtcaacgatt
    13141 ccaatgtgca aaaatccgcc atttcgcagt tgaaaagcgg gaaacgacgg atttttcttt
    13201 ataaaccagg ggataaaccg cctattttgg aagcattgcg gtcagggact tcgtcacatt
    13261 ctgaagctgc cccgcatgtc ccttgatctt gttcacatcg cctgtgcgca ggcctgactg
    13321 cacatcagtg atcgtcttgg atgtggacac ttcattatca atgatctttt gggttgtagc
    13381 ctcaagagtc acaagcatct gcatcacatt cttcggaatg atgggcaaat tcctgagaat
    13441 tggcaggaga atatccaaaa taccattggc ggtacgggca tatttaaccg tcaaagtgtg
    13501 cagggtacca atggaagtgg tcaactccag ggcgatctcc tggatcgaat cgatcattcc
    13561 tttgtgttgc tcaatgatct gcgctacatc gctcacagat ttcgtcaact gatcggaaaa
    13621 tttgatgatc gaacctgaac tggtctttgc ggcgatcaca ccgcttttag ctgggactct
    13681 ggtcggtact ggcatgggga aacctcctga aaataatgga tgacgcttac ctgaactcaa
    13741 aggtaacttc tcccaactat acaatacttc atttccaaaa acaaccttat tgacaaacat
    13801 ctgtatctcg tatatcctgc aaaacgactc acgcacccta actcggagga aaaaatgaac
    13861 caaatgattc aacttggaag tgtgaatggc tatcttgcaa aaccaggcgg tacgggcccc
    13921 ttccctgcca tgatcgtgat tcaagaatgg tgggggttgg atgcccaaac caaatccatt
    13981 gcggatcgtt ttgcacagga aggctacctc gcctttgccc cagaccttta tcacggtgaa
    14041 ctggcccagc ttggagatgg cgacaccgcg atgaaactcg tgcaaaaata cggccctgta
    14101 gcccataatg acttgacagc gctctacgac gcactgaaat cgcaccccga ttgcaatgga
    14161 aaaatcggca gtgttggctt ttgctttgga ggacgtatgt cactgagctt gagcaccctg
    14221 cgccctgtga atgcggtctg cacattttac ggcggcggta tgcaaatgat cttcgatcaa
    14281 ctgcgcgcca acctgaaagc ccccgtactt ggatttttcg gcgatgcgga tgtctcgatc
    14341 cctgcgggca ccatcgaaga atttgacaaa cttcttgacg aggttggcgt ggagcatgag
    14401 gtcatcacct accccaactc cggtcatgcc ttcttccgtg atagcgatcc cagtgtatac
    14461 attccagaag cctccaaaga tgcctgggaa cgtgcgaaaa aattcttcag caagaactta
    14521 aattagtcac tttccatcca ggaaacaaaa aacagcccat ttttcaaatg ggctgttttt
    14581 gttctgagcg ctaccctccg tttgctgcaa gcatctgcct gtaaggcaaa attaactcta
    14641 cgataaatac acacaccatc agaaataccc atagcaatgg aatgatccaa cgagtacgca
    14701 ggtgaagctt ttcagccttc cccaattcat cattattctt gtaaaccatc atggctactg
    14761 aaatgaccag cgaaacaaac accacagcat atgtaccgat cataaactta tcaatgaaag
    14821 tcaaataccc agtggcgggc acccctgaca ccaatgaaat gtgatacaac acaaccgcca
    14881 ccaaagtgga tgagaccaat gcaaggcgtt cgccagccgc ttcagccttc atcaaaaacg
    14941 acaacatacc gaccagtacg atcaccaggg cagcaaagat cgttttcata aaaccgctaa
    15001 agcggtcgcg ttccatgaac agccagatat acaagcggtc ataggaaata tcatcgtttg
    15061 gataaacaac aatatcgttc atatcgccat cgtattcagg gcggtaatac cagcctgacg
    15121 ggttaaatag catcgggtcc acttcaaccg cggcggaccg atataccaac tcgtcattgc
    15181 tatactcctt gtcttcgatg atgatatcca cataaagata atcgaacggg taaaacgtat
    15241 aatccacgta cccgatcagg gaggttttca agcgatagtc atatacttca aaaggaattg
    15301 aagtcccctg gtcttccggg tcgatgattt cggtggcgtt cattacatcc caatgcggct
    15361 cgtattggca tggcggggtc tcacatatga atgaaataaa aaaatcaacc gcgtacgtgc
    15421 ccgctgaaag gtcaatattg gaaatgttga gcacagtgat gtacacataa taaacatcag
    15481 ggccatccgc cttgccacct gcagactcac gcaccattcc atgtgttttg gacaaaggga
    15541 caaaagcaaa aagcaaaaaa gacagcagcc ccaaaataac gggagtaatt cgctgatgca
    15601 atgttttcat gttgcctcca gttaaaaaat tatagagaaa gtgtagcacg gctcgtttgg
    15661 caaatcaatt tacatccagt tgacaaatct tccgctctcc tttattctcg cttcatggcc
    15721 agcctcaacc tgcttcagcg tcaaatcatc gagtcacccc tcgactcgcg catcttcgtc
    15781 tcaggtcgtg cagggacagg gaagacaacc acaggggttg aacggatgcg cttcctgctt
    15841 gagcaaggcg tccccgccga ctcgatcctg atgctcacac cacagcgcac actgcaagaa
    15901 ccgtatctcg agttgcttta cagcccggag cgacgtgcag gtggtgaagt gacttccgcc
    15961 accattggcg gcctcgccaa acgcatgtgc gatctattct ggcccatcgc cgccgaagcg
    16021 gctggggtca aaaatcctga acgggctccc gttttcctca ctcttgaaac tgcacaatat
    16081 tacatggcat tcatcgtgcg cccgttgctg gaacagggct actttcaatc cctgaccatt
    16141 gaccgcaacc gcatgtattc gcagatcata gacaccctga acaaggcggc agtggttggt
    16201 ttcccttaca cagagatcgg cacacgcctt gatgccgcct gggtcggcga cccgggccag
    16261 cgccgcattt atcaagatgt gcaggattgt gccatccgtt tccgtgaata ttgccttgaa
    16321 cacaacctgc tcgatttctc ccttcaactg gagatattca ctgacattct ctggccgcag
    16381 gaacaggttc aaaactacct caaacagacc taccgccacc tcatctacga caacattgaa
    16441 gaagatgccc cgcgcgcgca tgacatcatc cgcgagtgga tgccagattt cgaatcggcg
    16501 ctgttgatct acgacaatga tgcggggtat cgccgcttcc tcggaggcga tgcagccagc
    16561 ggttatgccc tgatcgagac ctgtgagagt cagatcgaat tcagcgagag tttcgtctca
    16621 tccgaaaaca tcatccagct ttccaattca ctggtttctg cggtcacaaa tcaaagcacg
    16681 ggtgaagagg cctacccacc ggacacgatg gaattcatca tggcgcactt ctaccctgag
    16741 atgttgaatg aggtggttgg caaaataaaa acgctgatcg aagaaggtgt gccacccgcc
    16801 gagatcgtgg tgctggcgcc gtatctttca gacgcattgc gtttctcgat cgcagaccgt
    16861 ttggaaaagg aaaatattcc gtggcgctcg catcgtccat cgcgttcact gcgcgatgag
    16921 cctgccagca atgcgctctt aaatctttca gcacttgcac acccgcactg gaatgtgcgg
    16981 ccaacaaaat ttgacatgat gcaaaccctg atgttcgcat tggacacaga tctcgtgcgc
    17041 gcacaattgc tgaccgaagt ggtgtatgga caaagggatc tgcgtctttc gccttttgaa
    17101 gagatcaggc ctgagatgca ggagcgcatc acctacaggc tcggagaaaa atacacgacc
    17161 ctgcgcaact ggctgctcga atatcgtgaa gccgctcccc tgccgcttga tttctttttg
    17221 agaaaattat tcggcgaagt gatctcacag cccgaatacg gattccacag caacatggat
    17281 gcggtgcgtg tggcagcaag tctgatcgaa tccatcagga agtttcgcaa cgcgatggag
    17341 ccctttgatc aaacagggtt cgacatgggc agggaatacc tctccatgct ggcagatggc
    17401 gtgatcgcag cacaatatct tgaatcatgg cgcagcgaag ataaagaggc tgtgttggtt
    17461 gcacccgctc acaccttttt gatgatgaac cacccagtca ctattcaatt ttggctggat
    17521 cctggttctg tgggctgggt tcagcgcctt tcacagccgc tgactcatcc ctacgtgctt
    17581 tcgcgtggat gggacccgtc accagcacat ttctggatgg acaccgatga ggtacgcgaa
    17641 gagaccgacg cactggcaaa actggtcggc gggttgctca gccgatgcag ggaaaaagta
    17701 tttctggcac tggccgattt aagcgaatct ggaatggaac agcgcggttc tctattgcgt
    17761 gcgttccaaa aagtgttgtc actgcaggga caggaatgaa attcatttcc tgtaacgcgg
    17821 acttcagtcc gcagggtttt atgaccagga tgaaattcaa cacgcattcc agaaaaattg
    17881 gattggaatg aaaacacgcg aactcctgtg gattctcctc ctcgcggctc tgacgacgct
    17941 ggggcttgat cttgtcacga atgccatcaa cacgcaggga aatgcctggg atttccttta
    18001 ttacattgcg cttgccaaag atggatttca cgccgaccca ttggccagtc cgtttgcgta
    18061 tcgctacctg acacccttca ttgtccacgg gatttcgctg ctgggattct ccatcgaacg
    18121 cggattcggc atcatcgcct attttggtgc gatcatgcag ttgacgggaa tcttcttttt
    18181 catcaaatgg ctgggaaatt cgaccaaagg cgcttatctg gccatggcag tgacagcgct
    18241 ttcattgttc aatgtgaagt tcctgctttt cgatatttac aggcccgacc acctggccta
    18301 cgcactcatt cttttgcaaa cctatctggc attcaatcga aaattcctgc cattgctgtt
    18361 gaccaccctc attgcctcgc agattcggga attcaacatc attccgatgg ttgcctattt
    18421 atttatgtca tttcgcacca cagagcgtgg acttttcatc aagcaagccg ccatttcaac
    18481 aatctgcctc gtccccgcca ttctcctgcc gcgcctgctc atccctgtga cagaaaatta
    18541 ccaactcgtt gggctttcag gcgatgccct gctcaccacg gtcattttgc ccttcatccc
    18601 aagcgtggat gtgaatttca tcttctccat tttcgcctat cttttgccat tgctattcct
    18661 cgccgacctg aaaaccatca aagccgcatt cgcctccctg cctgaaaatc aacgcggata
    18721 tgtactgacc tacacggtac tcgtcctgct gctcagcttc ttcggcggca ccgatttcaa
    18781 ccgctttgcc accttcctct tcctgccgca aattattttg atcggcctca ttacacccgc
    18841 catctccagc cttcagatca gcctcatgct tgccgtcact ttcattttca accgcctctg
    18901 gatgcacatc cccgattggg acgtggaaaa gtaccgagat ttctacggcg gcttttcact
    18961 gcggttgaac atgtctacgc tgaatcggat catggaactg gccgcttttt gggcactcgg
    19021 tttcggtgtg agaaaatggc agggcacaac ccaaacatta gaatcacaaa ggcacgaaaa
    19081 caccacgcct caaagaaaat aactttgtgg cgatcaagag attctcgagg gcagtttgtc
    19141 tttttattcc ctgaattaca ccctgcaaga cctcatcggt tacacactcg cgttctttct
    19201 gtttccattg gtgatcgtgc tgcctgggta tgttttcagc tccgcactgg acctgttcga
    19261 tttcaaacgc cgtcttccca tcgtcaaatt cgggatcgga ttgctcgttt catttgcggt
    19321 ttctcccatt attttcaacc tcacttccag cctcggctca atgacctttt ccatcatcgt
    19381 cattggcggg tttgtgctgg cttttctctt ttttcttgca aaagaaaatt tcagcctgca
    19441 aattgccccg accgccaaac ctcttgtgtg gatatgcatc ggctgggtgg tgtttgtcgc
    19501 ttattcattg gtggatattc actggggcga ccagctttat gttagcgtgg tctccttcga
    19561 ccagacatca cgcgtctcgg tcattgatgc gatgacccgc acaggtgtgc cgccgatcaa
    19621 cccaagttac ttccctggtg cgccgaccct gctgacattt ttatattatt tctggtacat
    19681 cctcgccagt ctcgtggata tccttggcgg gactcttgtt gatgcgcgcg ccgcgctgaa
    19741 tgccagttcg gcatggtcag ggctggggct gatggcggcg atcgcttttt atcttcgggt
    19801 cagaaatgtc aatttgggca gggcagtttg gaggtcggca cgaatcgggg tcagcttgct
    19861 ggccgtcagc gggctcgatg tcctccccac aattatcatc atgctgggcg tgcgtcgtat
    19921 ctatggcacg gtcgaaggct ggaatttgca aatgcagatt cccacctgga attcatccat
    19981 tctgtgggcg ccgcatcaca ttgcagcgat ggtggcgtgt ttcaccgcca tcctgctggc
    20041 gctttcggcg cgcgaaaaac cattgcgaag aaaaatgacc ttgatgacga tcgcgggcat
    20101 ggcctttgca tcatcgttcg ggctttcgat ctgggtgaca tttttattcg gcgtattttg
    20161 ggcagtttgg gtgctcgcgt tgttcattca aaaaacggac cgcagcttgg tcgtgccgat
    20221 gatcttttgc ggtgttgtgg ctattctcct tgcgggctcg ttcatcctcg ggctgcttca
    20281 accgcaaggc gaaggcgggg taactcaatc cacggtgatc ttcgatgtgc gcactttgta
    20341 cttgcttgaa ggttttgtca ctgcatggcc gccgctcgcg cgctcattgc tcatgctggc
    20401 tctgctgccc ttgaattatt ttcttgaact tggctttttc attgttgcgg gttatttctg
    20461 ggtcagatca aaaaataaga ccgagatcgt ttccgatccg ttttatctgg ttgaggtcct
    20521 tttgatgagc gtcgtattgc tcatcggctc gaccctgaga tccaccatcg caaacaatga
    20581 cctcgggtgg agggtctggc tgcctggtca attcattttg ctgatctggg gcgtggatgt
    20641 gatcgagact ctcttttcga cgaagtccgc ctctgactca ccgaaagctg attctgtcaa
    20701 aagaaaaaat cttgcgctga cattgctcat cctcggcgtc ttgacctcct ctgtggatgc
    20761 cctcttcctg agagtgacct ggcccctgcg tgtcggcgtg gacgaaggca ggcgcggcta
    20821 ttctgcgcgg ctggcctacg attttctgcg cgatcaggtc cccgccgatg tcatcacgca
    20881 aaacaatccg cttccccttg ccgaccgtcc cagcgggtta tatggcacgc atcaaatggt
    20941 gatctccgac cgcaccattt atggcgtttc cctgacaacc tacgaccaat ttgttcatga
    21001 cctcgcgttc ctgtttaccg aaagtttttc aaactggcag cccatcgaca ccgcctgcca
    21061 aaaatattcc atcgacctgt tgatcttcaa cgatctcgac cccgcctggc ccagccttgc
    21121 cacgcttaaa acccagcgac ctgcattgta cgaaaatgag cattactcca tttttgcgtg
    21181 cggaagttat acgaaaatca ggtagcatgc accatgttaa aatgaacaag acaggcttcg
    21241 agcagcgcgg gaaattgcca ggaacacttt aaaggttata cgatgtcagg agagagaatg
    21301 aaaaacaaac aaatcccaac acttatcggt ttttttatat tgatgtatgc ctgcaatttt
    21361 ggaacgcctg ccagcatcac gcccacacca gatcgtgtcg aaacccaaca acctgcaacc
    21421 gctactgcgg aaatttcccc aaccactgcg ccttcccctt cgaatgggcc gactctcgca
    21481 aactgcccaa tgttccccgc agacaattat tggaacgcac ccatcgatac actcccgact
    21541 cattcacaat ctgaggcgtg gatcgacagt atcggccgca ctgtaagttt ccacatggat
    21601 ttcggctcag gcgaatacga tggcggcttg atcggaatcc catttaatat cgtgactgga
    21661 tcagctgtcc cgaaagttgg attcgagttt gattacgcag acgaatcaga tccaggtccg
    21721 tatccgattc ctgaaaatcc aaaccaggaa gcggggggcg accgtcacat tttggttttg
    21781 gataccgata cttgcacact ttacgaagtt tttgatgcca gtttcgatgg aggacaatgg
    21841 aggggcggct ctggtgcgat ctgggatttg aactcgaata ctctgcgccc cgatacatgg
    21901 acatcctccg acgcggcggg tctgccgatt ctgcctggcc tggtgcgcta tgatgaaatc
    21961 gctgcaggcg aaattaaaca tgcgctgcgt tttacgatcg aaaaaacggc tggctacatt
    22021 tggcccgcgc ggcatctgac agatgatcca caggatggga ttccgcccat gggtgcgcgt
    22081 tttcgtttga aagcagatta tgatatttca ggtttcccgc aggaaatgca aatcattctg
    22141 caagccatga aaacatatgg aatcattttg gcagacaacg gctcaaactg gtacatcagc
    22201 ggcgcgcccg atgaccgctg ggacaacgat atgctgcact tgcttgatgt attgacgggt
    22261 aatgatttcg aagccgtgga tacttcagga atgatggtgg atgtggattc gggaacggcg
    22321 aaataaggca ccagggccaa tgagtgaccc ctacgaattc atcgacatgg cccaaaaagc
    22381 cttcgatttc ctcgtgaagg acttcggctt tcaagtcact gtgacaaaat cacaaggaac
    22441 ctatcacagc gaggtcgttt ttacaacgca caaattgaag atcaccgtag gcatttgggg
    22501 tcaaagcaac gaggcgtgga tcatcttctt cccgcaggat aaaaactttc cacccctgca
    22561 cttctccagc ctcctaaacc gcatcacggg annnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22681 nnnnnnnnnn nggcaaaacg cacatcctct cggcgcttgc ggcgaagatc atcagggaag
    22741 gcgacctgca agatgggcag gaagtgctta tcgtcaccct cgtcaactcg gcagtggata
    22801 atttcgacaa gcgcatcaaa cagtttttca acaaccccat cgaatctttg tacaaatatc
    22861 gcgtacgcac cctgcatggg ctcgcgcacg acatcgtccg tgagaaacct gcgcgggtgg
    22921 ggcttgaaga acgcttcagc atcatcgacg agcgcgaagc gggctttatc cgccgcgaat
    22981 cggtcaatgc ctggctggca tccaactcgc tcgatgatta tctcgacccc gccctcgaca
    23041 attccaagca ggattggatt cgccgttcac aactgcccga tctggtggat tctcttgccc
    23101 tggcatttat ccgctctgcg aaagaccgcc tcctgactcc cgaaagcctg cgagccaaac
    23161 tggatgcatc tttcgccccg ctgccgctcg ccgaactcgg ctacagcatc tacgcggatt
    23221 atcaacgcgc cctcgcctac cgcggcgccg tggattttga tgacctcatc cgctatgcgt
    23281 tgaccctgct cgaaagcgac gaagaatttc tggaacgcct gcgttatcgc tacccctaca
    23341 ttttggaaga cgaagcacag gattcaagcc aaacccagga gaggatactg gctttattat
    23401 ctggcgaaaa ctgggtgcgt gtgggcgacc ccaatcaggc catctttgaa accttcacca
    23461 cagcctcgcc cgaactgctg cggaaattca tccgcgaaaa cccaagccag gatatgcccg
    23521 agtcggggcg ttcgcagccg tccattcttg cggtggcgaa ccatctcatt gactgggtgc
    23581 tcaacgccca tcccgacccc aacgtgcgga cggcgctatc gctgccatat atcatccctg
    23641 tccccaaaaa cgatccacag caaaacccgc ccgaaaaccc cgagggcatc aaattcatca
    23701 gcaggaaata ttcccctgaa gatgagcttg aagcggtggc caaatcggtg aaaaattttg
    23761 tcgcttcgat tgcagaaatc cccgatgaag aaaagccgac cattgccgtc cttgttccgc
    23821 gcaatgcccg cggcgtggaa gtggtcaacg aactgcgtaa gcgcggcatc gaaccgatcg
    23881 aattgatctc cagcacaacc gaaacccgcg cggcggcagg ctcgttgaat tatctgctgt
    23941 cgtatcttgc cgacccgcaa tctgcgcgga agttatcgaa ggcgtatgaa gtgtggcgaa
    24001 gggattggag agagcaggca acagggaatg atgattcaag ggctggggaa agcgaccccg
    24061 agttattgaa agaggtcact gccattttac gcaggttggt taatgtggaa aatttcatcg
    24121 cgccgagtca ggctgatgaa tggctggccg ccatcggtga ggtggattcg ccgcaagtca
    24181 tcgaggaact gggccttttc aaagtcaatg tgcagcgctg gctgaatgcg gctgtgcttc
    24241 cgatcgatca attggtgctg acgctggcac aggatgtgtt tggcaaggct tcggatctgg
    24301 cgttggctta taaactggcg ttggtgttac gcaaggccgc cgacgatcat gcagattggc
    24361 gcctgcctga attggcttcg gaactggccg tgatcgcgaa gaacgaacgg cgtttcatcg
    24421 gcttttcaga cgacgactct ggctttgacc ctgaacggca tcgcggcaag gtggtggtgg
    24481 tgacgatgca caaggcaaag gggctggaat gggaccgcgt gtacatgatg tcggtcaata
    24541 attatgattt cccgtccaac atgccgaatg accgttttat ttcggagaag tggttcgtac
    24601 gcagcgggtt gaatctcgaa gccgaggcac tggaacaact gaaggctctg gaatccaaca
    24661 gcgaatatga ctggtacgag gaaggcgcgg ccaccatgcg ctcacgcctt gattacgtca
    24721 aagagcgttt acgcctgttg tacgtaggca tcacccgcgc aaaaagggat ttgatcgtga
    24781 cgtggaattc aggccgtcag ggcgatgcaa cgccgagtct ggcgatgagt gagttgatgg
    24841 gatggtggga aaacaaatga tgaatgctga aggatgaaaa aaggtctcgg gatgtgataa
    24901 ttaatgctcg gtggtcaatg cctgagaagc aagtgccgca atctgagcat tatccacttc
    24961 aatgggcggg gagacaaatg tagttccatc ggcaaggtct tgttgcatgc gttgacgcag
    25021 tttttcgtaa ccctgatcgt tttttgagac aaagatcaat ttttggagca gatcgtttag
    25081 tttgcgccga atccacgctc cacgcgcttt cccccacaaa ttgatcactc gcgttccgcc
    25141 ctcgatgggc tccaacttga tcgtcatgct aaagctcgcg ccgataggcg tcacgccgtc
    25201 tgttgtatac ccctcaaagg gtt
//
LOCUS       DAOQJC010000054        24952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_882_length_24952_cov_8.618388, whole genome shotgun sequence.
ACCESSION   DAOQJC010000054 DAOQJC010000000
VERSION     DAOQJC010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 24952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24952
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_882_length_24952_cov_8.618388"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..505
                     /locus_tag="PLE39_09930"
     CDS             137..505
                     /locus_tag="PLE39_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68689.1"
                     /translation="MPNPSEFPIVFEKLKAILSAHTKKLTVKVDTPAAYSLDGPHSEK
                     WKKELFFGSVQVKKSYVSFYLMPVYMYPDLLKEISPELKKHMQGKSCFNFKTVEKSLF
                     EELSQLTKQGLERFMKEEYS"
     gene            complement(507..998)
                     /locus_tag="PLE39_09935"
     CDS             complement(507..998)
                     /locus_tag="PLE39_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNE68690.1"
                     /translation="MAKIDELVKDFLAQKKIAVVGVSDKRETGCNLGYKNFKGAGYQV
                     FAVNPRISTFEGDPCYADLKSIPEKPDAVFILASPKVTEQMVQQCVDLGIKHVWMHCM
                     MGTKPGLAASMTSVSSSAVEMCKANGITVIPGSCPNQFLNPDGGHKFMRGMWRLFGFM
                     SVN"
     gene            1267..>2660
                     /locus_tag="PLE39_09940"
     CDS             1267..>2660
                     /locus_tag="PLE39_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68691.1"
                     /translation="MTSTQKRVFTILIFISIAYFAAFIPPNNTGAKDQMMISLFEPDE
                     FAQYPVIIKMLNPGETLTATLFNFIAYDHYYYGSPFYFSSALLLLPIKLFDSLRNTTQ
                     LNMLLLRQVIGVLPMLGALLLLTHIQTKFSDYAKSIGLFVFLLSVSAVVENNLWWHVD
                     SLAVFFIVLTLFFLDRDDQRFGIHFALAAASTGLATGTKVIGLFFVLTIPTYLLVGIL
                     SKKLTWRDAFLKGLMFVGVMAAAIVISNPFLLLAGQRETMINTISRQASSQTEGWTLA
                     YAKGPASWKHIIEELYGQLLFVALSLVALGVGIWRSPNRIRHLLIATWAFPLGLYILF
                     TTAIKPTHFFLPILLPVYSSLIVFLEFPPFNPEKPRNAASLTWGAVLAAMVIWQFAIY
                     INKDVELYRDVLTREENEGSLVFYDTLERDYLPRITAAESLTVFRDVRMYFPDNSRWN
                     IRTYWNSKYSTIEK"
     assembly_gap    2661..2760
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2761..3035
                     /locus_tag="PLE39_09945"
     CDS             <2761..3035
                     /locus_tag="PLE39_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68692.1"
                     /translation="RWNIRTYWNSKYSTIEKIRPDIMILWSQRILDYTQEGALENAVD
                     PDSFQDTYTFYMDVKNEQVRGYHLIYRDAEGLMFVSDEIYELYFKE"
     gene            complement(3070..4581)
                     /locus_tag="PLE39_09950"
     CDS             complement(3070..4581)
                     /locus_tag="PLE39_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNE68693.1"
                     /translation="MKRIIKIILSGLISVTVLTSCIPAAVPTVYAPPTPINDHLPTVF
                     DIEIESPASPARLSDPRPNIIVVLTDDQPYHTVDYMPAVKNQLMKNGVVFENGFVTTP
                     LCCPSRVSILSGQYVHNHEVYTDRMPLGGAPKYDDSECVAVWLQEAGYRTAYFGKYLN
                     GYEDIEPYGVVPPGWDVWKAFLSKNLTADDDVGSTQYFFDFSLSENGTAVEYPRSKDN
                     FSADVITTDALEFIRDERDNPFFMMVGYYNPHSPYIAAPRHKDTFRASADEYWDWVQY
                     RPPNFNEEDIRDKPDYIGDLSPLSPDEVDTAHKQILRSLLSVDDGVASIMSALEKAGL
                     DENTIIVYVSDNGLTLGDHRFGAAKNCPYEACVKIPFIVHAPGRYAPRVDSHLVANID
                     LAPTIAEWAGVTAPAYVDGNSMVPILEDTSAGWREDILFEHWPTEEGVGSMIPEFYSI
                     RTADWKYTEYSTGEVELYDLVNDPFELENLAGKRDYREIQAELAARLQELKAE"
     gene            4664..5395
                     /gene="pgl"
                     /locus_tag="PLE39_09955"
     CDS             4664..5395
                     /gene="pgl"
                     /locus_tag="PLE39_09955"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNE68694.1"
                     /translation="MESKIHVYKNLQELSTAAADLFAASAAQAISSRGRFLAALSGGG
                     TPKPFHELLATAPYRDQVAWASTHLFWGDERCVAKDDSGNNYFQARQAFIDSVHLPEE
                     NIHRVLSELEPDSAADDYASQLKAFAEAPLAWPRFDLIILGMGDDGHTASLFPGSPVE
                     VNTPTVSVTANYQDRPARRVSLTPLVINAARRVVFLVSGQSKSETLARILHGEPQPDL
                     LPAQRIRPTDGEIIWLVDEAAAGKL"
     gene            5496..6407
                     /gene="gnd"
                     /locus_tag="PLE39_09960"
     CDS             5496..6407
                     /gene="gnd"
                     /locus_tag="PLE39_09960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HNE68695.1"
                     /translation="MELALIGLGKMGLNMATRLVRGGHRVVGYARTKETVETAIKNGA
                     EGAFTLEEAVGKLTSSPKVVWVMVPSGKTTTETIEKVASLLKPGDIIIDGGNSNYKDT
                     IAHAAMLEAKGIDFVDCGTSGGIWGITEGYSLMIGGKEEVTKKLNPIFETLAPGKELG
                     WGRVGPHGAGHYVKMVHNGIEYGMMQAFAEGFGILKGKKEFGLDLSQISHIWQHGSVV
                     RSWLLDLAANALDEDTELKEIKPWVADSGEGRWTVFESIDLDVPAPVITLALQMRFAS
                     RDEENYPARMLAALRNQFGGHAIKKAE"
     gene            6484..7962
                     /gene="zwf"
                     /locus_tag="PLE39_09965"
     CDS             6484..7962
                     /gene="zwf"
                     /locus_tag="PLE39_09965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNE68696.1"
                     /translation="MNNLPNNGLLTTIIIFGASGDLTQRKLIPSLFNLFRKRRTPKKF
                     QIIGFGGTAFTDEQFREHLHTGLKEYAGYTYTEEEWDIFAPNLHYLQGKYKEAEDFTK
                     LAEYLSTLEGGASNRLFYMALPPTLFPVIIDNLDASGQLHENGGWRRVVLEKPFGTDL
                     QSAQQLNLQVHKGLNESQIYRIDHYLGKETVQNILFTRFANTIFEPLWNRTYIDHVQI
                     TVAEKVGLEHRAGFYDGIGVLRDMFQNHLLQLLTLVAMEPPASFSASHLRNEKVKVLS
                     AIQPMTPEQVSANTVRAQYKGYRSEDQVNPNSTTPTYAALRLYINNWRWKGVPFYLRS
                     GKNLAEKQSQIIIQFKEPPLAMFPMQTMKPNMLVLYLQPDEGLHVRFEAKAPDTVAET
                     RSVDMEFRYSGAFGQTAIPEAYERLLLDAIQGDASLFTRADEVETAWSLIDPILQTWE
                     THQTPPLAVYRPGSWGPPEGYDLLARDGRRWLNEEASMMLEK"
     gene            complement(8040..10229)
                     /locus_tag="PLE39_09970"
     CDS             complement(8040..10229)
                     /locus_tag="PLE39_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNE68697.1"
                     /translation="MTKESLVLFPGRQEKEFPGDLLRSLTTSVLVLDLLFVLTSFLFF
                     PDLFQRALFFGGGVLLTCILVFLLIYMDKLKLAGVLLVSSLWLMVSYGAYTAGGVDSP
                     IFVGYAAVIMLGALVLGMKTGVAVIFLTIGFSGYLIYAEVNQILPQHISYQPVSRVFI
                     YIFFFFIVLLIQKAVVKTTRMAIGRLNSNESQYRSFLENIASVTYINDLSPDVLTTYV
                     SPQVKNILGYTQEEFLENPSLWMEILHPEDRERVFSESRRTSETGEAFMMEYRVMTKD
                     QRLVWVRDEAALVYDEKGLPQYWLGLWTDITNSKNSESAQLDTLGALLRRTNQLQTAS
                     EVSSAATSILELNELLPKVVELIRSHFDYYYVGIFLVDEKREMAILRAATGDAGRQLL
                     DARHALEIGNTSMIGWCVANNRARIALDVGKDAIRFKNPFLPLSRSELALPLRARGQV
                     IGAMTIQSVVEAAFTDADITALQTMADQVGNAIETARLFDERLRLINELETKNAELER
                     FSYTVSHDLKSPLVTIRGFVGYLWEDAKKGDLSRFEKDMQRIITATEVMQALLNDLLE
                     ISRIGRVTSPLEDIDFGEIVRETLNLILSPSQREMIRIEIQEEFPIVRCDRIRITEVV
                     QNLTANSIKFMGSQPEPKIQFGSAGVDAEKKYPIFFIKDNGMGIEPQHHERIFNLFSR
                     LNPEMEGTGVGLAIVKRVIEVHGGRIWLESQGENKGSTFFFTLPPAE"
     gene            complement(10367..12721)
                     /locus_tag="PLE39_09975"
     CDS             complement(10367..12721)
                     /locus_tag="PLE39_09975"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNE68698.1"
                     /translation="MYYMQGLTSLEAIAIWVVFGIAIIGLLYALFLRSQILREDKGTQ
                     KMQEVWGAIKDGADAYLRKQFRSILPLIGILTIALFLSVYVVPPSPEARERFSTMTES
                     QVRFWIGLARALAFIMGAGFSLTVGQLGMRMAVEGNVRVAAASKKSFGDALRVAYRAG
                     TITGMLTDGLGLLGGTIIFIILGIAAPDALLGFGFGGTLLALFMRVGGGIFTKAADVG
                     ADLVGKVEAGIPEDDPRNPAVIADLVGDNVGDCAGMAADIFESYEVTIVSGLILGLAL
                     WHTTGRLEWIIFPLMVRGIGVLSSIIGTYFVKGGETGKSEDAMKAIFSGFITSAGISA
                     ALFFIAGFFYMKDTMVDYGGWWRMPMAVGVGVLLAILIDRLTEYFTGTHASPVNDIKK
                     AADTGPATLILNGISVGFESSVWSVLVIALTIIASIFIFGTIPGVTGAERATFILYGV
                     AMTGIGMLTLTGNNVAMDSFGPIADNANGIGEMSWSGMEDKETKKAQQIMADLDAVGN
                     TTKAITKGVAIGSAVIAAVSLFGSFLVDVSRAQESLGIPVDQQIQAIGIRVDIPQIFV
                     GMLIGGALPWLFSSFAIQAVTRAASLIVLEVRRQFKLGVLDGKVKPDYAQAVNISTAA
                     AQKELVSLALLGIVTPIVVGLFLQVEALGGFLAGIIVSGQLLAVFLNNSGGAWDNAKK
                     LIEDEPKDPAKNLGKGSERHKAGVVGDTVGDPFKDTAGPALNPMIKVVNLVAVIIAPI
                     IVQYSTATGGVLFVVYGVAVVLLGVLAWAVVRSKAPAPSMTGDK"
     gene            12917..13330
                     /locus_tag="PLE39_09980"
     CDS             12917..13330
                     /locus_tag="PLE39_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNE68699.1"
                     /translation="MLQEIETQLFTLTPAASQAVKDILQQRNLEGYALRVYVAGGGCC
                     GVNFGMALDNNFRSVDTTFEANGVKVVVDEVSIDYLRDATVDFVNDPHHGQGFAVSSP
                     NAKGHSHGEGGCACSTNSGGSSCACGGGGACGCSN"
     gene            complement(13390..14025)
                     /locus_tag="PLE39_09985"
     CDS             complement(13390..14025)
                     /locus_tag="PLE39_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68700.1"
                     /translation="MIFARRIRLTAFILVMFLGMVFAFHSPVPTTVHAQQPTGSIPTV
                     TGTPGGATVALYLDEGHPQEYVYSGPSIYLYPAIGVLLAGQEVPAYGYSEDKNWIQIY
                     YPGVPNSVAWVYAPYVKVVKFGELPLVAAPPTPTPASTPVVNINPTLVAAFSTPVLPT
                     RLPTFTPPVPLVVATFVDEAVNTNRVPVGLLIFGFGFVGALGTIISFLRGR"
     gene            complement(14030..14641)
                     /locus_tag="PLE39_09990"
     CDS             complement(14030..14641)
                     /locus_tag="PLE39_09990"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE68701.1"
                     /translation="MKRIWFQPTLLIIFTTLFSLWTIFFASSSTVVLAQQPTETPAST
                     GMFITVITDEPQINVRMGPSSSIYPIVGTLLRGSTAPALGRSAGGDWVQIEFIGAPYN
                     KGWVYSPLVQVSPGTLLVVEAPPTPVPPPTSTVDPTLAAQFSIVPTSTRLPTFTPPPS
                     LVVPSYTQAPPVISSQTGSLAIIIIALAVLGMIGFLLSLFLRR"
     gene            14692..15276
                     /locus_tag="PLE39_09995"
     CDS             14692..15276
                     /locus_tag="PLE39_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE68702.1"
                     /translation="MERKIFHGNLKPVDVAQALLGEFNQGNLQAQSFGQSDKMIVQVG
                     THPGAMSGGQTAMTVTIQKHEDGIMVELGQQAWLGVAASLGMSALAALRNPFSLLGRL
                     DDIAQDIENLQMSEKIWQVINQTARAAGASTELSDRLKRLTCEYCGTANVVGDPSCVA
                     CGAPLGKVQPRTCRHCGYVARPDDKKCQNCGQDL"
     gene            15299..16117
                     /locus_tag="PLE39_10000"
     CDS             15299..16117
                     /locus_tag="PLE39_10000"
                     /inference="COORDINATES: protein motif:HMM:NF023818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 domain-containing protein"
                     /protein_id="HNE68703.1"
                     /translation="MNLKELEARLQALIEVDLLKILPSRKVEDVIVQKLAAAIQLNTT
                     TATDGSVIAPNVYTLIVHPSEAAKWQDQQLLTILLHSISAVAQEAGFKVTISPTITIS
                     TDEKLPLNDAQVVASYRIEPMADTNATPLDTGNLEDYSKIPENAFLIVEGVKVFPLKL
                     PVINIGRRLDNQLIIDDPRVSRNHAQLRAIKGRFVVFDLNSTGGTFVNGQRASQVVLY
                     PGDVISLAGVALIFGQDNPPPRPDLKDTAPFQSATADRPTASLKEHSTAKLKRR"
     gene            16114..16584
                     /locus_tag="PLE39_10005"
     CDS             16114..16584
                     /locus_tag="PLE39_10005"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNE68704.1"
                     /translation="MSAPIILALRLMMALALYGFLGWALFVLWRDVQKQGFSLANRRV
                     PGISLTVRHGRGAPIVKSFTQPEITIGRDPGCDIPLIDDTISTRHAQLTYHHGQWWLD
                     DLASTNGTALNNIMVTMSTVLTSGDEIQCGDTRLIINLSTDTFVSPTQRVERHQ"
     gene            16581..17450
                     /gene="mtnP"
                     /locus_tag="PLE39_10010"
     CDS             16581..17450
                     /gene="mtnP"
                     /locus_tag="PLE39_10010"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNE68705.1"
                     /translation="MNMEKASIALIGGSGLYQMAGLQNTTEHEIDTPFGKPSSPIVIG
                     TLEGTRVAFLARHGIGHHITPTEVPYRANIYALKLLGAERIISISACGSLREDYAPGD
                     IVIPDNIYDNTKDRVRSFFGNGFVAHVGVADPFCTDLSNDLEAAVRATNAKTHRGGTL
                     ITIEGPRFSTKAESNAFRAWGMSIIGMTASPEAFLAREAEICYATMAHVTDYDVWHES
                     EEPVTVEMVIQTLNKNTQKAQEAIHNLIRGLKEQRNCKCGEALATALITNPKVIPTET
                     RQNLDLLVGKYMK"
     gene            17655..20087
                     /locus_tag="PLE39_10015"
     CDS             17655..20087
                     /locus_tag="PLE39_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNE68706.1"
                     /translation="MHDQIQTRLLRWAGIFLLIQSTILTLAPAVRERSWDVDYRLSHW
                     VGLIAWAVFMTAAHRASIKHLPERDPYLLPAAALLSGWGLLTIWRLDETFGIRQTIWL
                     GLSVIGFILAMSLPQNLFFLRRYKYVLLGGGLLITALTLLLGTNPLGVGPRLWLGCCG
                     VYFQPSEPLKLLLVIYLSAYLADRINIRSSSLSLLVPTLVVTGVALLLLLVQRDLGTA
                     SIFICIFTVIIFLSTGRRRILFGAVFFLALALFLGYFFIDIIRIRVEGWLDPWADPSG
                     QSYQIVQSLLAVANGGTIGRGPGLGSPLLVPVAISDFIYAAIAEETGLAGTIGLLGTI
                     WLLLARGMISSLRAADRFRRYLAAGVVTYLGIQSLLIIGGNLRLVPLTGVTLPFVSYG
                     GSSLLTSFIALFILLTISNLEDSEPAQLQDPKPYTFLAGLLALGLATSALTTAWWAVI
                     RAPDLLNRTDNPRRAIADRYVPRGELVDRDNQPINITEGESGTYQRVYVYTELAPITG
                     YTHPIYGQAGLEATLDDYLRGLQGNPASLLWWDRLVYGTPPPGLDVRLSLSLALQSKA
                     DQLLGGHSGAILLMNAETGEILAMASHPTYDPNKLDEEGEALSQATNSAAPLVNRATQ
                     GLYPIGTAMLPLIRANFGETQPTDNELKAYYQKLGLYQAPQINMPVAYDTENPSAYEL
                     RVSPLQISLAASVLSNHGMMVVPSIATAVNTPEQGWVVLSTAESRPVEMIQAGAADEA
                     ALSFILEGKPFWSHVGQASGSNDTVVTWFLGGTLPSWSGAPLTVVVTLEENNTFLAKY
                     IGENLLDTATSR"
     gene            complement(20151..21797)
                     /gene="pckA"
                     /locus_tag="PLE39_10020"
     CDS             complement(20151..21797)
                     /gene="pckA"
                     /locus_tag="PLE39_10020"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNE68707.1"
                     /translation="MNNLLNIKTPAESIAQTRKADYNLSNNGIGNLRLAYWNLPTEAL
                     VEEAIFRNEGALVAGGPFVANTGKHTARSANDKFVVRHTDSENNIWWGVYNRPFEADK
                     FEVLYDRMLGFLQGRDVFVQDVYGGADESYRLPVRIVTELAWHSHFVRNMFILPQSLE
                     EYKRFVPEFTIIAMPSFKGAPAVDSTNSETFICLSFEKKLAIIGNTAYAGEIKKSVFT
                     ILNYLLPLDGVLSMHCSANVNPDNADDVALFFGLSGTGKTTLSADPTRRLIGDDEHGW
                     SDDGVFNFEGGCYAKVIGLSESAEPEIYATTKRFGTILENVPFDPVTRSIDLDDDTIT
                     ENTRASYPLEFIDNAVPEKKAGHPKNVILLTCDASGVMPPIARLTPNQALYQFISGYT
                     SKIAGTEVGLGKEPEITFSACFGGPFMVHHPYKYAELLKNKIERYGVTCWLVNTGWVG
                     GPYGVGKRISIKYTRALLNAALNGKLDQVKFTHDPIFGFEVPTQCPDVPAEVLNPASS
                     WHDQKEYDRRYKDLAMRFKQNFGKFEIGTPQDVIEAGPKV"
     gene            complement(21892..23109)
                     /locus_tag="PLE39_10025"
     CDS             complement(21892..23109)
                     /locus_tag="PLE39_10025"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNE68708.1"
                     /translation="MKLSKLALEIAESPTLALNEEARLLRERGEAVINLGIGEPKNKT
                     PIAAILASGAKLSSGEVKYTPPDGLPSMKKAVIRYTEENYNRLVAPENVIITNGAKQS
                     LFNIFYSILNPQDEVIIIAPFWVSYPEMIKMCLGVPVVVTPEDGTFTPRFEDIERAIT
                     SSTRAIVVNSPNNPSGAIYPPELVEKIVELCERKGIFMICDDIYHKLVFGRNVAVPAY
                     SFTKKEIDNSHIIVVNGVAKVYGMTGFRIGWVIAPRDLIKVMTNVLAQTTSCVSPIAQ
                     AAAEGALNGLQSVVEALRLQIQNNREVVLQEMRTFNGARLIEPKGTFYALLDLRAFNT
                     NSVELSKFLLKKALVVTVPGREFGMEGHIRISFAGSIKDITEGIARIKWALDPTSPNE
                     IYIGDKKMIRDWM"
     gene            complement(23365..24621)
                     /locus_tag="PLE39_10030"
     CDS             complement(23365..24621)
                     /locus_tag="PLE39_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNE68709.1"
                     /translation="MKKILLPFILITLIVAACEPLPTTPQFVTQIPFYASQTPEFIIP
                     VTDTPIVSEPFTETPAFIVPVTETPTPEFIIPVPETPTPVPLAGWWEVYFTDPLAINN
                     PDAFAGSIEEKLIQWINAAQVSIHIASFEFNLTPVAEALIAAKNRGVDVKWITDDEYG
                     LKIDSEPERRQFSLLMGAGIEVKDDAGREAFMHNKFWIFDQQAVWTGSTNITVNGIFK
                     QNNNVIVLRSPEIAYIYEREFQELWSGQFGPRAPSTISNQWAILDTTPVQVLFSSEDN
                     AMSSLLAVVNDAKFSIRFLAFSFTDDALAQTMIERFKAGVDVQGVYETLASTSIYSDL
                     KTFWCAGVPVRQDGNGSLMHNKVIIVDNSTVITGSLNFTAGANEKNEENVIIIDNPEI
                     AALYLQEYAKIWSLAHDVEAGTFTCQ"
     gene            complement(24707..>24952)
                     /locus_tag="PLE39_10035"
     CDS             complement(24707..>24952)
                     /locus_tag="PLE39_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl CoA-acyl carrier protein transacylase"
                     /protein_id="HNE68710.1"
                     /translation="PVIGNVHAAPLADAEAARADIIAQMQSRVRWTESVQYMAANGVN
                     TFVEVGTGSVLGGLVKRIADGAGIYPLGNPQDFAALE"
BASE COUNT         6283 a         6841 c         6056 g         5672 t
ORIGIN      
        1 cgcaaccgaa ggcccctacg aaagcgcctg gcaagcctac gacccagagg gaaatccttt
       61 cggcgacccg atatttatca agattgttgt tgtgccgtaa accctcccac tccgctcaaa
      121 tactcaggag agaaaaatgc ccaacccatc cgaattcccc atcgtcttcg aaaaactaaa
      181 agccatcctc agcgctcaca ccaaaaagct gaccgtcaaa gtggataccc ccgccgcata
      241 ctcgctcgac ggtccccaca gcgaaaaatg gaaaaaagaa ctcttcttcg gctcggtgca
      301 ggtcaaaaag agttacgtct cgttttacct catgcccgtt tacatgtacc ccgacctgct
      361 caaagaaatc tcccctgaac tcaaaaaaca catgcaggga aaatcctgct tcaacttcaa
      421 gaccgtcgaa aaatccctgt tcgaagagtt aagtcaactc accaaacagg gactcgaacg
      481 atttatgaag gaagaatatt cctaattcaa ttcacgctca taaacccaaa caacctccac
      541 atcccccgca tgaacttatg cccgccatcg ggattcaaaa actgattcgg gcacgacccc
      601 ggaatcaccg tgatcccgtt cgccttgcac atctccaccg cagatgatga aacgctggtc
      661 atgctcgccg ccaaccctgg ctttgtgccc atcatgcagt gcatccacac gtgcttgatt
      721 cctaagtcca cacattgctg caccatctgc tcggtcacct tcgggctggc aaggataaat
      781 accgcatcgg gcttctcagg gatggatttc agatccgcat aacacggatc gccctcaaaa
      841 gtggagattc gcgggttgac cgcgaacacc tgataccccg cacccttgaa gttcttataa
      901 cctaaattac agcccgtctc gcgcttgtct gaaacaccca ccaccgcgat ctttttctgc
      961 gcgaggaaat cttttacaag ttcgtctatt tttgccattt caaaattctc cttgatggaa
     1021 cctacgaaat tataggcatt ccgttccgca aaatgcaggt gacaaaaatc acctttatct
     1081 ccttccaaac gccaaagcaa aaagttttta tcaggaaaac gcccgtcaac cgtaggtcgg
     1141 attggcaatc cgacccacat agactcaaag aaaaaaaact tagtgacttg gtgccttggt
     1201 ggctttgtgg tctacaacac tttacttatg caagaggtca aatccaaaaa tggtatcatt
     1261 gcccgcatga cttccactca aaaacgcgtc ttcaccatcc tcatttttat cagcatcgca
     1321 tatttcgccg cctttatccc ccccaacaac accggcgcca aagaccagat gatgatctcg
     1381 ctcttcgagc ctgacgaatt cgctcaatac cccgtcatca tcaaaatgct caaccccggc
     1441 gaaaccctca ccgccaccct cttcaacttc atcgcctacg accactacta ctacggctca
     1501 cctttctact tctccagcgc cctgctgctc ctgcccatca aactcttcga cagcctgcgc
     1561 aacaccaccc aactcaacat gctcctgcta cggcaggtca tcggcgtttt gcccatgctt
     1621 ggcgcgcttc ttctgctcac ccacatccaa accaaattca gtgactacgc gaagtctatc
     1681 ggtctttttg tattccttct ctcggtttcc gccgtggttg aaaacaacct atggtggcat
     1741 gtggatagtc ttgcggtctt cttcatcgtg ctgactttgt tcttccttga tagagatgac
     1801 caacgcttcg gcattcactt tgccctcgcc gccgcttcca ccggactggc aactggcacc
     1861 aaggtaatcg gccttttctt cgttttgacc attcccacct acctgctcgt cggcatcctc
     1921 tccaaaaaat tgacctggcg ggacgctttt ctcaaaggcc ttatgttcgt cggcgtcatg
     1981 gcggcagcca tcgtcatctc caatcccttc cttttattag caggtcaacg cgagacgatg
     2041 atcaacacca tctcacgtca ggcctcctcc caaaccgaag gctggactct ggcttatgcc
     2101 aaaggccccg cctcatggaa acacatcatc gaagaactct acggccaatt gcttttcgtc
     2161 gcattgtcgc tcgtggcgct tggggtcggc atctggcgca gcccgaatcg gattcgtcac
     2221 ctgctcatcg caacctgggc atttccgctt ggcttgtaca ttctcttcac caccgccatc
     2281 aaaccgacgc attttttcct gcccattctg ctcccagtgt acagcagcct gatcgttttc
     2341 cttgaattcc cgccgttcaa ccctgaaaaa cctcgcaatg ccgcatcgct aacctggggc
     2401 gcagtccttg ccgcaatggt catctggcaa tttgcgatct acatcaacaa agacgtggaa
     2461 ctgtaccgcg atgtgctcac ccgtgaagaa aatgaaggct ctctcgtttt ttacgacacc
     2521 ctcgaacgtg actatcttcc acgcatcacc gccgccgagt cgttgaccgt cttccgcgat
     2581 gttcgcatgt acttccccga taactcccgc tggaacatcc gcacctactg gaacagcaaa
     2641 tacagcacca tcgaaaagat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 cccgctggaa catccgcacc tactggaaca gcaaatacag caccatcgaa aagatccgcc
     2821 ccgatatcat gatcctctgg tcacaacgca tccttgatta cactcaggaa ggcgcgctcg
     2881 aaaacgcggt tgaccctgat tcatttcaag acacctacac cttttacatg gatgtcaaaa
     2941 acgaacaggt ccgcggttat cacctgatct atcgggacgc cgaaggcctt atgttcgtca
     3001 gcgacgaaat ttacgagctg tattttaaag aataacagtg tattgcaaga taaatgttgc
     3061 ggcacaaaat cactcggcct tcaattcctg caatctcgcc gccagctcag cctggatttc
     3121 cctgtaatcc cgcttccctg ccagattttc caactcaaac gggtcgttga ccaggtcgta
     3181 caattccact tcacctgtgc tgtattcggt gtatttccaa tcagcagttc ggatcgaata
     3241 aaactcggga atcatcgagc cgacgccttc ttccgtaggc caatgctcaa ataaaatatc
     3301 ctcccgccaa cctgcagacg tgtcttcaag gatcggcacc atactgtttc catccacata
     3361 cgcaggcgca gtgacaccag cccattcggc gatcgtgggg gcaagatcaa tgttcgcaac
     3421 gagatgcgaa tccacccgtg gggcgtacct acccggagca tggacgatga acggtatctt
     3481 gacacaggct tcatacggac aatttttcgc cgcaccaaaa cgatgatcac ccaatgtcaa
     3541 accgttatca gaaacataaa caatgatggt attttcatcc aggcctgcct tctcaagtgc
     3601 agacataatg gaagcgacgc cgtcatccac tgagagcagc gagcgtagaa tctgcttgtg
     3661 tgcagtatca acttcatcgg gagaaagagg cgagaggtcc ccaatgtaat caggcttgtc
     3721 gcggatgtcc tcttcattaa aattcggcgg acgatattgg acccaatccc aatattcatc
     3781 tgcactggcg cggaaggtgt ccttgtgccg cggcgcagcg atgtatggcg aatgcgggtt
     3841 gtagtagccg accatcataa aaaacggatt gtcgcgttcg tcgcgaatga attccagggc
     3901 atcggtcgta atcacatcgg cgctgaaatt atctttcgag cgcgggtact ccacagcggt
     3961 cccattttcc gaaaggctga aatcaaaaaa atattgtgtg ctgcccacat catcatcggc
     4021 agtgaggttc ttgctgagaa aagccttcca cacatcccac cctggcggaa caaccccata
     4081 cggctcgata tcttcgtaac cgttcaggta ttttccaaaa tatgccgtgc gatacccagc
     4141 ctcttgcagc cacaccgcca cacattctga atcatcatat ttgggcgcac cgcccaacgg
     4201 catgcggtcg gtatagactt cgtgattgtg aacatactgc cccgataaaa tactcacacg
     4261 gcttgggcag cacagcggtg tggtgacaaa tccattttcg aacacgaccc cgtttttcat
     4321 caattgattt ttcacagcgg gcatgtaatc caccgtgtga tatggctggt catcggtaag
     4381 caccacaatg atgttcgggc gcggatcgct caaacgcgca ggcgaggcgg gtgattcgat
     4441 ctcaatatca aaaaccgtcg gaaggtgatc gttgatcggc gtgggcggag catacacggt
     4501 cggcaccgca gcagggatgc aggaggtcaa cacggtgaca ctgatcaggc cgctcaagat
     4561 aatttttata attcgcttca tctattctcc aggcaggttg ggttgcaaag tgaattgtaa
     4621 caagaactta taaattcaac ccgcatcgtg ataaactcgg gcgatggaat ccaaaattca
     4681 cgtctataaa aatcttcaag aattaagcac cgcagcagca gacctattcg ccgcgtctgc
     4741 ggcgcaggcg atcagttcac gcgggcgttt cctcgcggca ctttcaggcg gaggcacgcc
     4801 caaacctttc catgaattgc ttgccaccgc accttaccgc gatcaagttg cctgggccag
     4861 cactcacctc ttttggggtg atgagcgctg cgtggcaaaa gatgattcgg gaaataatta
     4921 ttttcaggca agacaggcat ttattgacag cgttcacctg cctgaggaaa atattcaccg
     4981 cgtcctctct gaactggaac ctgattctgc cgccgatgat tatgccagcc agttgaaagc
     5041 atttgccgag gcgcctctcg cctggccccg ctttgatctc atcatcctcg gcatgggcga
     5101 tgacgggcat acggcctcgt tattccccgg ctccccggtg gaggtcaaca cgccaacggt
     5161 ttctgtcaca gccaattatc aagaccgtcc tgcgaggcgt gtctcgctga caccgttggt
     5221 catcaatgcg gctcggcgtg tggtctttct ggtcagtgga caaagtaagt ccgagaccct
     5281 tgcccgcatc ttacatgggg aacctcagcc agacctgtta cccgcccaac gcatccgccc
     5341 cactgatgga gaaatcatct ggctggtgga tgaagcagcg gctggcaaac tataaaaaat
     5401 tctcgggtga aaatcatcct ctcaaaacac aggaccgcag actttagtcc gctgcccttg
     5461 aaatcgagtg gaaataaaat ttaggagcaa agaatatgga actcgcactg atcggcttgg
     5521 gtaagatggg attaaacatg gcaacacggc tcgtacgcgg aggtcatcgc gtggttgggt
     5581 acgcccgcac caaagagacc gttgaaacag ccatcaagaa tggcgctgaa ggcgcattca
     5641 ccctggaaga ggctgtgggc aagctcacct cctctccgaa agttgtctgg gtgatggtgc
     5701 cctcaggcaa aaccaccaca gagaccattg aaaaggttgc ttccttatta aagcctggcg
     5761 atatcatcat tgatggcggc aactcgaact acaaagacac catcgctcat gcagccatgc
     5821 ttgaagccaa aggcatcgac tttgtggatt gcggaaccag cggcggcatt tggggtatca
     5881 ccgaaggcta cagcctgatg atcggcggca aggaagaagt gaccaaaaaa ttgaacccga
     5941 tcttcgaaac gctggccccc ggaaaagaat tgggctgggg acgtgtcggt ccgcatggcg
     6001 caggtcatta tgtgaaaatg gttcacaacg gcatcgaata cggcatgatg caggcctttg
     6061 cagaaggttt tggaattttg aagggcaaga aggaattcgg cctcgatctt tcacagatct
     6121 cgcacatttg gcagcatggc agcgttgtcc gctcgtggct gcttgacctt gcggcaaatg
     6181 ccctcgatga agataccgaa ttgaaggaaa tcaaaccctg ggtggctgat tccggcgaag
     6241 gccgctggac ggtcttcgaa tccattgacc tggatgtgcc tgcccccgtc atcacccttg
     6301 cactgcaaat gcgctttgcc agccgtgatg aagaaaacta ccccgcccgt atgctggctg
     6361 cgcttcgcaa ccaattcggc ggacacgcca tcaagaaagc tgaatagacg ctagatagac
     6421 gatggaccgt aggccgtggg aacttttcgc ggtccatcgt ccactgtcca cggtcattta
     6481 tttatgaata acctacccaa taatggctta ttaacaacga tcatcatctt cggtgcctcg
     6541 ggtgacctga cccagcgcaa actgattcca tcgctcttta atttgtttcg caagcggcgc
     6601 acaccgaaaa agtttcagat cattggcttc ggtggaaccg cctttaccga tgagcagttc
     6661 cgcgagcatt tgcataccgg gctgaaagag tatgctggct acacctacac cgaggaagaa
     6721 tgggatatct ttgcgcccaa cctgcactac ctgcagggaa aatacaaaga agccgaagat
     6781 ttcaccaagc tggccgaata cctgagcaca cttgagggcg gcgcgtccaa tcgcctgttt
     6841 tacatggcgc tcccgcctac gctgttcccg gtcatcattg acaatctcga tgcatcaggt
     6901 caactgcatg aaaatggcgg ctggcggcga gtggtattgg aaaagccgtt tggcaccgat
     6961 ctgcagtcgg cacagcaatt gaatctgcaa gttcataaag gcttgaacga atcgcaaatc
     7021 taccgcatcg atcattacct tggtaaagag accgtgcaga acattttgtt tacccgcttt
     7081 gccaacacga tctttgaacc actctggaat cgaacataca ttgaccatgt gcagatcacc
     7141 gtcgctgaaa aggtcggtct cgaacatcgt gcagggtttt acgatggcat cggcgtgctg
     7201 cgggatatgt ttcaaaatca tctcttgcaa ttgctcacgc tggttgccat ggaaccgccc
     7261 gcgtcgttca gcgcaagtca cttgcgcaat gagaaagtaa aagttctcag cgcgatccag
     7321 cccatgacgc ccgagcaggt ttcggcaaat acggtgcgcg cacaatataa aggctatcgc
     7381 agcgaagacc aggtcaaccc gaattccacc acgcccacgt atgcggcgct ccgtctctat
     7441 atcaacaact ggcgctggaa aggcgtgccg ttctatttgc ggtctggaaa aaacctcgcc
     7501 gaaaaacaat cgcagatcat tattcaattc aaggaaccgc cgctggcgat gttccccatg
     7561 cagacgatga agccgaacat gcttgtgctt tatttgcagc ccgatgaagg gttgcatgtc
     7621 cgctttgagg caaaggcgcc agacacggtt gcagagacac gatctgtaga tatggaattt
     7681 cgttactcgg gggcgttcgg gcaaacggcg attccagaag cctacgaaag gctgttgctg
     7741 gatgccattc aaggcgatgc ttcgctcttc acccgcgcag atgaagtgga aacggcatgg
     7801 tcgttgatcg acccgattct gcaaacatgg gaaacgcatc agactccgcc gttggctgtt
     7861 tacagacccg gtagctgggg cccccccgaa ggatacgacc tgcttgcccg tgatgggcgg
     7921 cgctggctca atgaagaagc cagcatgatg ttggaaaaat aaataaaaag tcgaggtgcg
     7981 tgcaatggca tctcgacttt ttatttccat taaatttctg gaacgctttg gacgattatt
     8041 tattccgcag gcggcagagt gaaaaagaaa gtgctgcctt tgttctcccc ctgcgactcc
     8101 aaccatatcc gcccgccgtg gacttcaatg acgcgtttta cgattgcaag gccaaccccg
     8161 gttccctcca tttcggggtt caagcggctg aacaagttga aaatccgttc gtgatgctgg
     8221 ggttcaatgc ccatcccgtt atccttgatg aagaaaatgg gatatttctt ttcagcatcc
     8281 acacctgctg agccgaactg gattttcggc tcaggctggc tgcccatgaa tttgatggaa
     8341 ttggcggtca ggttttgcac gacttcggta atgcggatgc ggtcacagcg aacgatcggg
     8401 aattcttcct gtatttcaat tcggatcatc tcccgttgag acgggctgag aatcaaattc
     8461 aaggtttccc tgacgatctc tccaaaatca atatcctcca gcggactggt cacacgcccg
     8521 atccgcgaaa tctcgagcag gtcgttcaac agggcctgca tcacttcagt ggcagtgatg
     8581 atccgctgca tatctttttc aaagcgcgat aaatcccctt ttttggcgtc ttcccacaaa
     8641 tatccgacaa atccgcgaat ggtcacaagc ggcgatttta aatcatgtga cacggtatag
     8701 gaaaaacgct ccaattccgc attcttggtt tccaattcgt tgatcaatcg caaacgttca
     8761 tcaaaaagac gcgcggtctc aatggcattt ccaacctggt ccgccatggt ctgcagggcg
     8821 gtaatatcag cgtcagtgaa ggccgcctcc accactgatt gaattgtcat cgcccctatt
     8881 acctgtccac gggcacgcaa tggaagtgcc agttccgagc gggaaagcgg caaaaaagga
     8941 ttcttgaaac gaatggcatc cttccccaca tccaacgcaa tacgcgcccg attgtttgcc
     9001 acacaccagc cgatcatgga ggtatttccg atctcaaggg cgtgtctggc atccagcagt
     9061 tgcctgccag catcacccgt ggccgctcgc agtatcgcca tctcgcgctt ttcatccacc
     9121 agaaaaatgc cgacgtaata gtaatcaaaa tgtgaacgga tcaactccac cactttggga
     9181 agcaattcgt tcaactccaa aatggacgtt gctgcactcg aaacttcact ggcggtttga
     9241 agttgattgg tgcgccgcag aagtgcgccc agggtatcca actgtgcgct ttcagaattc
     9301 ttactgttgg ttatatcggt ccacagcccc agccaatatt gcggtagtcc tttttcatca
     9361 tagaccaggg cggcttcatc ccgcacccat acaagccttt gatccttcgt catgacacgg
     9421 tattccatca tgaaggcttc accggtctca gaggtccgtc ggctttcgga aaaaacccgc
     9481 tccctgtctt ctgggtgcaa aatctccatc cacagggagg gattttccaa aaactcctcc
     9541 tgggtatatc ccaatatatt tttcacctgc ggactgacat aagtagtaag cacatctggc
     9601 gataaatcat tgatataggt aaccgaggca atattttcca aaaaagatcg atactgactc
     9661 tcgttcgagt tcagccgccc aatggccatg cgagttgttt tcacgacggc tttttgaata
     9721 agaagaacga tgaaaaagaa gaaaatataa ataaaaaccc tggaaacagg ttgataagaa
     9781 atatgttgag gcagaatctg gtttacttct gcatagatca ggtacccgct aaagccaatc
     9841 gtaagaaaaa tgaccgctac ccctgttttc attcccaata cgagggcacc gagcataatc
     9901 actgctgcat aaccgacaaa aattggtgaa tccaccccac ctgcggtata cgccccatag
     9961 gagaccatca gccaaagaga agacaccaaa agcaccccgg ccagcttcag cttatccata
    10021 taaattaaca aaaagaccag aatacaagtc agtaagacac ccccgccaaa aaacaaagct
    10081 cgttgaaaca ggtcgggaaa gaataaaaac gaagtcagaa caaacagcag gtccaaaaca
    10141 agaacgctgg ttgtaaggga acgcaacaag tcacctggaa attccttttc ttgtctaccg
    10201 gggaataaca ccaaggactc ttttgtcatg ttttatctta aagctctgcc caattttatc
    10261 ttagtttccc atttccctat tttttaaaaa ccatacaggt ttcgtccaaa aaagaagact
    10321 ggcgattttg atcgccagtc ttcttttttc atatattacg gtttatctat ttgtcgccag
    10381 tcatggatgg agcaggcgcc ttggagcgaa caaccgccca agccagaacg ccaagcaata
    10441 caacagccac gccataaaca acgaacaaaa cgccgccggt cgctgtggag tattgaacaa
    10501 tgatcggggc aatgatcacg gcgaccagat tcacaacctt gatcatcggg ttgagtgcag
    10561 ggccagccgt atccttgaac ggatcaccga cggtgtcacc caccacacca gccttgtggc
    10621 gttcagaacc cttaccaagg tttttggcag gatccttggg ctcatcttcg atcagtttct
    10681 tggcattgtc ccaggcgccg ccggagttgt tgaggaacac agccagcaac tggccggaaa
    10741 cgatgatgcc cgccaaaaag ccgccgaggg cttccacttg caggaacaag cccacaacga
    10801 tcggggtcac aataccgagc aatgcgaggg ataccaattc cttctgcgca gcagcggtgg
    10861 agatgttgac ggcctgagcg taatcaggct tgacctttcc atccaacaca cccagtttaa
    10921 actggcggcg gacttcaagc acaatgagag aagcagcgcg ggtcacagcc tgaatggcga
    10981 aagaggagaa cagccagggc aaagcgccac cgatgagcat acccacaaaa atctgcggga
    11041 tatccacacg gataccaatt gcctgaatct gctggtcaac aggaatgcca aggctttctt
    11101 gagcgcggga aacgtccaca aggaaggaac cgaacaagga aacggcagca atcaccgctg
    11161 agccaatggc cacacccttg gtaatggcct tggtggtgtt acccaccgca tccaggtcag
    11221 ccatgatctg ctgggccttt ttggtttcct tatcttccat gccagaccat gacatttcgc
    11281 caatgccgtt cgcattgtct gcgatcgggc caaaggagtc cattgccacg ttgttgccgg
    11341 tcaaggtcaa cataccgata ccggtcatgg ccacaccgta aagaatgaag gtcgcgcgtt
    11401 ccgcacccgt tacaccgggg atcgttccga aaataaagat ggaagcgatg atggtcaacg
    11461 caatgaccag aacagaccaa acggacgact cgaaaccaac ggaaatacca ttcaagatca
    11521 aggttgcagg gccggtatct gcggccttct tgatgtcatt cacaggagag gcatgtgtac
    11581 cagtaaagta ttcggtcagg cgatcgatca agatcgcaag caacacaccc acacccacag
    11641 ccatcggcat acgccaccag ccaccgtaat caaccatggt gtctttcatg taaaagaaac
    11701 cagcaatgaa gaacaatgcg gcggagatcc cagcagaagt aataaagccg ctgaagatgg
    11761 ccttcatcgc gtcttcgctc ttgccagtct cgccaccctt cacaaagtag gtgccgataa
    11821 tggaagacag cacgccaata ccacgaacca tgagggggaa gatgatccac tcaaggcggc
    11881 cggttgtgtg ccagagggcc agaccaagaa tcaggcccga tacgatcgtc acttcatagg
    11941 attcgaaaat gtcagcggcc ataccagcac agtcaccgac gttatcgcct acgaggtctg
    12001 caatcacagc aggattgcgc ggatcgtcct cagggatgcc tgcttccacc ttaccaacaa
    12061 ggtcagcgcc gacatctgca gccttcgtga aaataccacc acccacgcgc atgaacaacg
    12121 cgaggagtgt accgccgaaa ccgaagccaa gcagggcgtc aggagcggca ataccaagga
    12181 tgatgaagat gatggtaccg ccaagcagac ccaaaccgtc tgtgagcatg ccggtgattg
    12241 tgccggcacg ataggcaacg cgcaatgcat caccaaaaga cttctttgag gcggcagcca
    12301 cacgcacatt gccttccaca gccatacgca taccgagctg acccacagta agtgagaacc
    12361 cagcgcccat gataaaggcg agtgcgcgcg ccagaccgat ccagaaacgg acctggcttt
    12421 cggtcatggt ggagaagcgt tcacgggctt caggagatgg cggaacgaca taaacggaca
    12481 ggaacaaagc gatcgtcaaa atgccgatca gaggcagaat ggagcggaac tgcttgcgca
    12541 ggtaggcatc tgcaccatct ttgatcgcac cccagacttc ctgcatcttt tgagtgcctt
    12601 tatcttcacg caggatttgg gagcggagga agagcgcgta caacaagccg ataatggcaa
    12661 tgccaaatac gacccagatc gcgatcgcct ccaatgaggt aagtccttgc atataataca
    12721 tgtgcggtat atcctccggg gggaataaaa aatgttggct tgcgcctatt aagcagaggg
    12781 attttacagt tgcaagatga atgtgtcaag aaatgtggat gtcaaatcaa gttttattga
    12841 cacacgttga cagtccatcc caaatctgat aatatctagc gaaattagat gtaaactcaa
    12901 aatcaggaga gaaaacatgc ttcaagaaat tgaaacccaa ctgttcacat taactcctgc
    12961 cgccagtcag gctgtcaaag atattcttca acagcgcaac cttgaaggtt acgccctgcg
    13021 tgtgtatgtt gcaggcggtg gttgctgcgg cgtaaatttt ggcatggcgc ttgacaacaa
    13081 cttccgcagt gtggatacca cattcgaagc caacggcgtc aaggttgtgg ttgatgaagt
    13141 ttccattgac tatctccgcg atgcgacggt cgatttcgta aacgacccac accacggaca
    13201 gggatttgca gtcagcagcc ccaacgcaaa aggacactcc cacggtgaag gcggttgcgc
    13261 ctgcagcacg aacagtggtg gttcatcctg tgcctgcggc ggcggcggtg cctgcggttg
    13321 cagcaactag gctttttcga atcaaaaaat gaccgggtaa caccggtcat ttttatttaa
    13381 tggcacggat catcttccgc gcaggaaaga aatgatcgtt cccaatgcgc cgacgaaacc
    13441 gaaaccgaaa atcaagaggc ctaccggcac acgattcgtg ttgactgctt catctacgaa
    13501 agttgcaacc accaaaggga cgggtggagt aaaagtgggc agccttgtcg gcaaaacggg
    13561 ggtgctgaac gccgcaacca aggttgggtt gatatttaca acgggtgtag atgcaggggt
    13621 tggggttggc ggagctgcca ccaaaggcaa ttcaccgaac tttacaactt ttacataagg
    13681 tgcatatacc caggctactg agttaggaac accgggataa taaatttgaa tccaattttt
    13741 atcttcagag tacccgtagg caggaacttc ctgacccgcc agcaaaaccc caatggccgg
    13801 gtaaagataa atactgggac ccgagtaaac atactcctgt gggtgtcctt catccaaata
    13861 caaggctaca gtggcgccac cgggtgttcc agttacagtt ggaattgaac ccgttggctg
    13921 ctgggcatgg accgtggtcg gcacagggga atggaatgca aacaccatgc ccaaaaacat
    13981 gaccaaaatg aatgccgtaa ggcgaattcg tcttgcaaag atcatattat tatcggcgca
    14041 aaaataggga aagcaggaag ccgatcatac ccaaaacggc aagcgcaata atgataattg
    14101 ccagagatcc agtctggctg gaaatgaccg ggggagcttg tgtgtatgaa ggaaccacca
    14161 atgagggcgg aggcgtgaag gtgggcagac gtgtagacgt tgggacaatg ctaaattgcg
    14221 ccgccagagt tgggtcaacg gttgaagttg gcggcggcac aggcgtgggc ggggcttcca
    14281 ctactagaag cgttcccggg gagacctgca caagcggcga atacacccaa cctttattgt
    14341 aaggagcgcc gatgaattca atctgcaccc agtcgccgcc tgcagaccgt ccaagcgcag
    14401 gggcagttga ccctctcaac agggtgccga caatggggta aatggaagag ctcggtccca
    14461 tgcgcacatt gatctgcggc tcatcggtga tgactgtgat gaacattcct gttgaagcag
    14521 gagtctctgt cggctgctgg gccaggacaa cggtcgagga agacgcgaaa aaaattgtcc
    14581 agagggaaaa caaggttgtg aaaatgatca ggagggtggg ttgaaaccag attcgcttca
    14641 tttcgtaagt aactcttaag gagaatcgca aaggcgatta taatctactt catggaacga
    14701 aaaatttttc acggaaatct caaaccagtg gatgttgcac aagctctgct gggtgaattc
    14761 aatcagggaa atttacaggc acagtccttt ggacaaagtg acaagatgat cgttcaggtg
    14821 ggaacccacc ctggggcaat gtctggcgga caaacggcca tgactgtgac gatccaaaag
    14881 catgaagatg gcatcatggt ggaattgggc cagcaggcct ggcttggcgt ggctgccagt
    14941 ttgggaatga gtgcgctcgc tgctctgagg aatcccttca gcttgcttgg tcggctggat
    15001 gatattgccc aggacatcga aaaccttcag atgagtgaaa agatctggca ggtgatcaac
    15061 cagactgcgc gcgccgccgg ggcatccact gagttatcgg accgcctcaa gagattgacc
    15121 tgtgaatact gtggaacagc aaatgttgtt ggcgaccctt cgtgtgttgc ctgcggggct
    15181 ccgcttggca aagtgcaacc gcgtacctgc cggcattgtg gttatgtagc ccggcctgat
    15241 gacaaaaagt gccagaactg cggccaggac ctttaataat aaagattaac aggaaaaaat
    15301 gaatctgaaa gaactcgaag cccgtctgca agctctgatc gaagtggatt tattgaaaat
    15361 tctccccagc cgcaaggttg aagatgtgat cgtgcaaaaa ctggcggcgg ccattcaact
    15421 taataccacc actgccaccg atggctcggt cattgcaccc aatgtttata ccctgatcgt
    15481 gcatccaagc gaggccgcca aatggcagga ccagcaattg cttacaatcc tattgcattc
    15541 gatctcagcc gtcgcccagg aagcggggtt taaggttaca atatctccaa cgatcacgat
    15601 ttcgacagac gaaaaacttc cattgaatga tgcccaggtc gtggcatctt atcggataga
    15661 acctatggca gacacaaacg caaccccact tgacactgga aatctggaag attacagcaa
    15721 gattcccgaa aatgcttttc taattgtaga gggagtcaag gtttttccgc tgaaacttcc
    15781 tgttatcaat attgggcgca gactggataa ccaactaatc attgatgacc cgcgggtctc
    15841 acggaatcat gcccaactgc gtgcgatcaa aggcaggttc gtggttttcg acctcaattc
    15901 aacaggcggc acctttgtga atggacagag ggccagtcag gttgtgttgt accctggtga
    15961 tgtcatttca ttggccggtg tggccctgat ctttggtcag gacaacccgc ctccccgccc
    16021 agacctgaaa gataccgctc ctttccaaag cgccacagca gatcgcccca ctgcgtcgct
    16081 caaagaacat tccacagcta aattaaagag aagatgagcg ccccgatcat attggcttta
    16141 cgccttatga tggcccttgc cctgtatgga ttcctgggct gggcactttt tgtattatgg
    16201 cgtgatgtgc aaaaacaagg cttctcactg gcaaatagac gagttcccgg aatcagcctc
    16261 acggttcggc atggacgcgg ggcacccatc gtaaaaagtt ttacccaacc tgagatcacc
    16321 attgggcgcg accccggatg tgacatccct ttgatcgatg acaccatctc cacccgtcac
    16381 gcgcaattga cctatcacca cgggcaatgg tggctggacg atctcgcatc caccaatgga
    16441 acagccctga acaacatcat ggtcaccatg tcaacagtgc tgacctctgg cgacgaaatt
    16501 caatgcggcg acacccgcct catcatcaat ctttcaaccg acactttcgt atccccaaca
    16561 caaagagtgg aaaggcatca atgaacatgg aaaaagcatc catcgcgctc atcggcggct
    16621 ctggcttgta tcaaatggct ggcttgcaaa acacaaccga acatgaaata gatactccct
    16681 tcgggaaacc cagttccccc atcgtcatcg gcactttgga agggacgcga gtcgcctttc
    16741 ttgcaagaca cgggatcggt caccacatca caccgaccga agtcccgtac cgcgcaaaca
    16801 tctacgcctt gaaactgctc ggcgcggagc ggatcatcag catcagcgcc tgtggatctc
    16861 tgcgtgaaga ttacgcgccc ggtgacattg tcatccccga taacatttac gacaacacca
    16921 aagaccgtgt ccgctccttc ttcggaaatg gatttgtggc gcacgtcggt gtggcagacc
    16981 cgttttgcac cgacctctca aacgaccttg aagcagccgt ccgtgccacc aacgccaaaa
    17041 cccatcgcgg cggaacatta atcaccatcg aagggccgcg cttctccaca aaagcggaat
    17101 caaatgcctt ccgtgcctgg ggcatgtcca tcatcggcat gaccgcctcc ccggaagcct
    17161 tcctcgcgcg cgaagcagag atctgttatg ccaccatggc acacgtcacc gactatgatg
    17221 tctggcatga aagcgaagag cccgtcaccg tggaaatggt catccagacc ctgaacaaga
    17281 atacacaaaa agcacaggaa gccattcaca accttatccg cggattgaag gaacagcgca
    17341 attgcaaatg tggtgaagca cttgccacag cgttgatcac caacccgaaa gtgatcccaa
    17401 ctgaaacacg ccaaaacctc gatctgttgg ttggcaaata tatgaaataa cgcttcttga
    17461 gcaggcagct ttatccatca ttctaaaagt gggtcaaagc cgcactcaaa aaattaccag
    17521 caagagccat gaataaaaag ccctgacacc aataatcttg atgcagggct ttttccttgc
    17581 ctcgtacaca aaccgccgtt ttcctgaaga aacccaattt ccactcccat cgaaaaatga
    17641 tgtaccatta atgaatgcat gaccaaattc aaacccgcct gcttcgctgg gctggaattt
    17701 ttctcctcat acaatccaca atcctgacgc tggcaccagc cgtacgcgaa cgttcctggg
    17761 atgtagacta caggctctca cattgggtcg gtctcattgc atgggccgtg ttcatgaccg
    17821 ctgcccatcg cgcttccatc aaacacctgc ctgaacgcga cccttacctg ctgcctgctg
    17881 cggctttact tagtggctgg ggactactca ccatctggcg gttggatgaa accttcggga
    17941 tcagacaaac catctggctt gggctcagtg tgataggctt catccttgca atgtctctgc
    18001 cgcaaaatct tttttttctc aggcgatata aatatgtatt actgggtggc ggactgctca
    18061 tcaccgcgct tactttattg cttggcacaa atccgcttgg cgttggtccg cggttgtggc
    18121 tgggatgttg cggcgtgtac tttcaaccct ctgagccgct caagttactg ctggtcattt
    18181 atctctccgc ctacctcgcc gaccgcatca acattcgctc gtcgtcactc tctttgcttg
    18241 tgcccacgtt ggtcgttaca ggagttgcgc ttttgctcct gctcgtccaa cgcgatctcg
    18301 gcaccgcgtc cattttcatt tgcattttca ccgtcatcat ttttctttcc acaggcaggc
    18361 ggcgcatttt gtttggcgcg gttttctttt tggcgctcgc gctcttttta ggatattttt
    18421 tcatagacat cattcgcatc cgcgtggaag gctggcttga tccgtgggcc gacccatcgg
    18481 gacagtccta ccagatcgtg caatctcttc tggcggttgc gaacggcggc accatcgggc
    18541 gcggacctgg attgggaagc ccgttgcttg tgcctgttgc catctctgat ttcatctatg
    18601 cggccattgc ggaagaaacg ggtctggctg ggaccatcgg cctgctcggc accatttggc
    18661 tgctcctcgc ccggggcatg atctcctcac ttcgcgccgc agacagattc cgccgttatc
    18721 ttgctgcagg tgtggtgacc tatctcggca ttcaaagcct gctcatcatc ggcggcaatt
    18781 tacggctggt accgctgacg ggggtcacac ttccgttcgt ttcctacggc ggctcatcgc
    18841 tgctgacatc tttcatcgcc ctcttcattc tgctcaccat cagcaacctc gaagacagcg
    18901 aacccgccca attgcaagac cccaagccat acactttcct tgcgggtctg cttgccctcg
    18961 gactcgccac cagcgcattg accaccgcat ggtgggcggt gatccgcgca cctgacctgc
    19021 tcaaccgtac cgacaatccg cgccgcgcca ttgcagaccg ttacgtgccg cgcggcgagt
    19081 tggtggatcg ggacaaccaa cccatcaaca ttaccgaagg cgaaagcgga acctatcaac
    19141 gtgtgtatgt gtacacagaa ttggcaccca tcactgggta cacccatccc atctatggtc
    19201 aagctgggtt ggaagccaca ctcgatgatt atttgcgcgg cttacaggga aacccagcca
    19261 gcttgttatg gtgggatcgt ctggtttacg gcactcctcc gcctgggttg gatgtacgtc
    19321 tcagcttgag ccttgccctg caatccaaag cggaccaatt gctgggcggc cacagcggag
    19381 ccatcctttt gatgaatgca gaaacaggtg agatccttgc catggcgtcg cacccaacct
    19441 atgaccccaa caagctggat gaagaaggcg aagccttgtc acaggcgaca aattcagcgg
    19501 cacccctcgt gaaccgtgcc acgcaggggt tgtacccgat cggcacggcc atgctgccgt
    19561 tgatccgcgc gaattttgga gaaacgcaac cgacagacaa cgagttgaaa gcgtattatc
    19621 aaaaactggg cttatatcag gcgccgcaga tcaatatgcc cgtcgcttac gatacggaaa
    19681 atccatccgc gtatgaatta cgggtcagcc cgttacagat cagcctggcc gcatctgtgc
    19741 tgagcaatca cggcatgatg gtagttccga gcattgccac cgcagtgaac acgcccgagc
    19801 agggatgggt ggtgctttcc acagcggaga gtcggcctgt tgagatgatt caggccgggg
    19861 cggcggatga agcggcgctt tccttcatcc tggaaggcaa accgttttgg agtcacgttg
    19921 gtcaggccag tgggtcaaac gataccgttg tcacgtggtt tctcggcggc accctgccca
    19981 gttggagcgg cgccccgctg accgtggtgg tgacgctcga agagaacaac acatttctcg
    20041 caaaatacat cggcgaaaac ctgctcgaca cggcgacaag taggtagaaa aacagagcag
    20101 gcctttgtgc ctgccctgtt tcacttttca ctcgctactt tccaccctcc ctataccttc
    20161 ggccctgcct cgatcacatc ctgcggggtg ccgatttcga attttccaaa attttgttta
    20221 aagcgcatcg cgaggtcttt gtatcttcgg tcatattctt tttggtcatg ccatgatgaa
    20281 gcagggttca aaacttcagc gggcacatcg gggcattggg tggggacctc aaagccaaag
    20341 atcggatcgt gcgtgaattt gacctgatcc agttttccat tgagcgcggc gttcaacaaa
    20401 gcgcgcgtgt acttgataga aatgcgcttg cccacaccat acggcccgcc cacccaacct
    20461 gtgttgacca gccagcaagt cacaccgtaa cgctcgatct tgttcttgag caactcggca
    20521 tatttatatg gatgatgcac catgaacggt ccgccaaagc aggcgctgaa cgtgatctcg
    20581 ggctctttcc ccaacccgac ttctgtccct gcgatcttgg aagtgtaccc gctgatgaat
    20641 tgatacaaag cctgattcgg ggtcaggcgc gcaatcggcg gcatcacccc gctggcatca
    20701 caagtgagca ggatgacatt cttcgggtgt cccgccttct tttcaggcac ggcattgtca
    20761 atgaactcaa gcggatacga agcacgcgta ttttcggtaa tcgtgtcatc gtcgagatca
    20821 attgagcgcg taacgggatc aaagggcaca ttttcgagga tcgtgccaaa gcgtttcgtc
    20881 gtggcgtaga tctctggttc cgcagattca gacaacccga tgacttttgc gtagcatcca
    20941 ccttcaaagt tgaacacacc gtcatcgctc cagccatgtt catcgtcacc gatcaggcgg
    21001 cgggtggggt cggccgaaag cgtggtcttg cccgttccgc tcaggccaaa aaacaaagcg
    21061 acatcgtccg cgttatcggg gttcacattg gcagagcaat gcatcgaaag cacaccatcg
    21121 agcggaagca gataattcag gatcgtaaaa acggacttct tgatctcgcc tgcgtaagcc
    21181 gtattgccga tgatggcgag cttcttttca aaggacaggc aaatgaaagt ctcgctgttg
    21241 gtgctgtcca ctgcgggcgc gcccttgaac gacggcatgg cgatgatcgt aaactcaggc
    21301 acgaagcgtt tgtattcctc aagcgattgc ggcaggatga acatgttgcg cacgaagtga
    21361 ctgtgccatg ccaactcggt cacaatgcgg acaggcagac gataggattc atctgcgccg
    21421 ccgtagacat cctgcacaaa gacatcacgt ccctgcagga aacccaacat tcggtcatac
    21481 aggacctcga acttgtccgc ttcaaaagga cggttgtaca cgccccacca gatgttgttc
    21541 tcggagtcgg tatgccgcac cacgaattta tcgttcgcac tgcgggcggt gtgttttcct
    21601 gtatttgcca caaagggacc acccgcaacc aacgcgcctt cattgcggaa aatcgcttct
    21661 tccacaagcg cctcggtggg caggttccaa taggcaagcc gtaaatttcc aattccgtta
    21721 ttcgataagt tgtaatccgc tttacgcgtc tgcgcgatgg actcagcggg ggttttgata
    21781 ttcaacaggt tattcataag ttagtctccg tttgggagtc aggttgaagg tttcaggttg
    21841 aagggtgaaa gtttgcgacc agtaccttga aacatgtcac ctgaaactct ttcacatcca
    21901 atcccgtatc atcttcttat cgccaatgta aatttcattc ggcgaagttg ggtcgagtgc
    21961 ccatttgatg cgggcaatgc cttcggtgat gtcttttatg ctgcccgcaa acgaaatgcg
    22021 gatatgcccc tccatgccaa attccctgcc tggcacggtg accaccaacg cctttttcag
    22081 cagaaacttg gaaagctcaa ccgaattcgt gttgaatgcc cgtaaatcga gcagggcata
    22141 gaacgtccct ttgggctcga tcaggcgcgc cccattgaaa gtccgcattt cctgcaagac
    22201 cacctcacgg ttattttgaa tctgcaaacg cagcgcctcc accacagact gcaagccgtt
    22261 cagcgcacct tctgccgcgg cctgtgcgat cggtgacacg caagaggtcg tctgtgcaag
    22321 gacattggtc atcactttga tgagatcgcg cggagcgatc acccagccga tgcgaaaccc
    22381 cgtcatgcca tagaccttcg ccacaccgtt gacaacgatg atgtgagaat tgtctatttc
    22441 ttttttggtg aacgagtacg cagggacggc aacatttcgg ccaaagacca gcttatgata
    22501 gatgtcatcg cagatcataa agatgccttt gcgctcacac agttccacga tcttttcgac
    22561 caattcaggc gggtagatcg cgcctgacgg attgttgggg ctgttcacaa cgatcgcccg
    22621 tgtggatgaa gtaatggccc gttcgatatc ttcaaaacgc ggagtgaagg tcccatcttc
    22681 gggtgtgacc accacaggca cgccgaggca catcttgatc atttcagggt aagagaccca
    22741 aaacggcgca atgatgatga cctcatcctg tgggttcaaa atggagtaaa agatattgaa
    22801 aagcgattgt ttcgcgccat tggtgatgat cacattctca ggcgcaacca gtcggttgta
    22861 gttctcttcg gtgtatcgaa tgacagcctt cttcatcgaa ggcaagccat cgggcggtgt
    22921 gtatttcacc tctccgctgc tcaattttgc gccagaagcc agaatggccg caatcggtgt
    22981 tttattcttt ggctccccga tccccaaatt gatgaccgcc tccccgcgtt cacgcaacag
    23041 acgcgcctcc tcattcaagg ccagggtcgg cgattcagcg atctccagtg caagtttgct
    23101 tagtttcata agtctccttg atatgtcagt gtgacgcgga ttttttccca gctacgcctg
    23161 ctccgtgccc acaatgacat ttcgttaaaa taaaaatctg gtcaggcaat tagccgacca
    23221 gatacatttc cataaccgca ggaagaaagc ggcactgaca atcaacttgt caggcatggc
    23281 gttcatgacg tcattatact atacgggttt aaatgccctc tcgaacgaaa atcccatttt
    23341 ccttgtaaca aatgtaacgc aaacttactg gcaggtaaaa gtccctgcct ctacatcatg
    23401 ggccaaactc cagatcttcg cgtattcctg caaatacagc gcggcgatct cggggttgtc
    23461 aatgatgatg acgttctctt catttttttc attcgcaccc gccgtgaagt tcagggaacc
    23521 tgtaatgacg gtgctgttat ccacaatgat cactttgttg tgcatcaggc ttccattgcc
    23581 atcctgcctc acaggcaccc ctgcacacca aaacgttttc aaatctgaat aaatgctggt
    23641 gctggcaagc gtttcataca caccctgcac atccacccca gccttgaagc gctcgatcat
    23701 ggtctgtgcc agggcatcat cagtaaaact aaaagcaagg aagcggatgc tgaacttcgc
    23761 atcgttcacc acggccagca ggctgctcat ggcgttatct tcggaagaga ataaaacctg
    23821 aactggggtt gtatccaaaa tcgcccattg attgctgatg gtcgaaggcg cacgcggacc
    23881 gaattgcccg ctccacaact cctggaattc acgctcgtaa atgtacgcga tctctggcga
    23941 acgcagcacg atcacattgt tgttctgttt gaagatgccg tttaccgtga tattggtcga
    24001 acccgtccac acggcctgct gatcgaagat ccagaacttg ttgtgcatga aggcttcccg
    24061 ccccgcatca tccttgacct cgatgcctgc tcccattaaa agggagaatt gtctacgctc
    24121 gggctctgaa tcgatcttga gaccgtattc gtcatccgtg atccatttca cgtccacgcc
    24181 gcgattcttt gctgcgatca atgcttcggc cacaggcgtg agattgaatt caaacgacgc
    24241 aatatggata ctgacctgag ccgcattgat ccattgaatc aacttttctt caatggaccc
    24301 tgcgaacgca tcggggttat tgatggcaag cggatctgta aaatagacct cccaccagcc
    24361 tgccagcgga acgggggtcg gcgtttcggg cacagggatg atgaattcag gagtgggagt
    24421 ttcggtgaca gggacgataa acgctggtgt ttccgtgaaa ggctcagaca cgatcggggt
    24481 atcggtcact gggatgataa attcgggtgt ctgactcgcg taaaaaggga tctgtgtgac
    24541 gaactgcgga gttgtcggta gcggctcgca ggcggcaaca attaaagtaa taaggatgaa
    24601 tgggagaagg atttttttca taataagtgt agagcgacat ggtatgtcgc tctaccaaat
    24661 ataccgcttt tgcgagaata acgcggaagt tcgcccgcga gccaatttac tctagtgccg
    24721 caaaatcctg cgggttgccc agcggataaa tgcccgcccc atcggcaatg cgtttgacaa
    24781 ggccaccaag cacagaacct gtgcccacct ccacaaaggt gttgacgcca ttggcagcca
    24841 tgtattgcac cgattcggtc cagcgcacgc gggattgcat ctgtgcgatg atgtcggcgc
    24901 gggcggcttc agcgtcggcc agcggcgcgg catgaacatt tccaatgaca gg
//
LOCUS       DAOQJC010000055        24752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_889_length_24752_cov_10.727376, whole genome shotgun sequence.
ACCESSION   DAOQJC010000055 DAOQJC010000000
VERSION     DAOQJC010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 24752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24752
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_889_length_24752_cov_10.727376"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..672
                     /locus_tag="PLE39_10040"
     CDS             325..672
                     /locus_tag="PLE39_10040"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNE68711.1"
                     /translation="MNSQSTADWLFFLLYNLNMTRRTVITRIRKHRAQLTKLGVKSLS
                     LFGSVARGEEGPNSDVDILVEFKGKATLDRYMDTKFYLEDLLGCKVDLVTPKAIKPRM
                     KPYIMQDLVHVTG"
     gene            659..997
                     /locus_tag="PLE39_10045"
     CDS             659..997
                     /locus_tag="PLE39_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNE68712.1"
                     /translation="MSRDESLYLADVQESCEKVLRFTKGMTYKDFVNDDLHFDAVLRN
                     LEIIGEAVKNISEATRQKYPDVKWRKIAGFRDIVAHEYFGINDETVWDIVENEVPALL
                     AVMKIMLEEK"
     gene            complement(1077..1448)
                     /locus_tag="PLE39_10050"
     CDS             complement(1077..1448)
                     /locus_tag="PLE39_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68713.1"
                     /translation="MDASSPVQPNPQKRSNRPIAALLLSLSSFFFCCISVLVNNPNGF
                     DAFMEGKPSWYIAQALMCLAALLPLIGAILGILSLRAGEINRNLAIAAIVLGVIGFFE
                     SLGIFGYVMVFVGIINVFSPP"
     gene            complement(1470..3194)
                     /locus_tag="PLE39_10055"
     CDS             complement(1470..3194)
                     /locus_tag="PLE39_10055"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HNE68714.1"
                     /translation="MENFIGKHIDRYNIIDLLGQGGMATVYRAYDTRLEREVALKVIR
                     HEAFPPDVLQNELKRFEREAKSLARLSHPNIVKVLDYGEYEGSPFLVMEYLPGGTLKQ
                     KIGSPIAWQDAMHLLLPVARGVEYAHNREIIHRDIKPSNILLAENETPTLSDFGIAKL
                     FQNEGTLLTASGMAMGTPEYMAPEQWTGKTVPQSDMYALGVVLYEMVTGRRPYTADTP
                     GGVFMMQVTEPVLFPRDIVPHLPEAVEQFLLKALSREPSNRHASMTEFIRDMELLLSN
                     AETKVPINRMETLKGSPGQSSVRKDASSPSRKLVPFNWKAATLGGVILLAVVVAAAVV
                     LMKKKSTPLAAEITDSKNAVMVLVPEGDFVMGSDNGDEDERPVHTVYLDEFYIDKYEV
                     TNLDYRVCVVDGVCKPPQDMTNYNNPEYENHPVVYVDWEMARTYCDWRDAQLPTEAQW
                     EKAARGMDARTYPWGEGIACSQANYLSCIEDTQPFDSYAGSLSPYGAYNMAGNVWEWV
                     ADWYSPAYDVSETDNPVGAESGERRVLRGGGWNQHEYLLRTSARLGVVPTDVFLSFGF
                     RCASDVNQ"
     gene            complement(3262..>3924)
                     /locus_tag="PLE39_10060"
     CDS             complement(3262..>3924)
                     /locus_tag="PLE39_10060"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE68715.1"
                     /translation="IPAGDFNMGSEGGDADELPIHLVRLDAYSIDVYEVTNAAYQACV
                     DASICKPPISTGSLTREEYYGVSEYENFPVINIDWDRASTFCAWRGGRLPTEAEWEKA
                     ARGTDNRIYPWGDAPDCAKSNCTADTLEVGSFGEGKSPYGVYNMAGNVWEWVADWYSR
                     EYYSISPLENPQGPSSGESHVLRGGAFDATERLKRVSARGAYFPEDFFSRVGFRCAAD
                     AP"
     assembly_gap    3925..4024
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4025..4673)
                     /locus_tag="PLE39_10065"
     CDS             complement(<4025..4673)
                     /locus_tag="PLE39_10065"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE68716.1"
                     /translation="MSQTPRPLKVFLCHSHSDRTAVRALYSRLVLDGVDAWLDKEKLL
                     PGQEWELEIRRAVRESDVVVICLSNQFNQAGFRQKEVRLALDTAMEKPEGEIFIIPAR
                     LEECESLASLSKWQWVDLFEENGYEKLLRALVLRSDRIRANARSLMRFDAKAEKRRQV
                     VFLVQQAEKMEKDADWMAAIENYRQVNRIDPAYFGVEEKIASLQQKMQAGTVTVTL"
     gene            complement(4745..6682)
                     /locus_tag="PLE39_10070"
     CDS             complement(4745..6682)
                     /locus_tag="PLE39_10070"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE68717.1"
                     /translation="MPDLVGKSLGRYHIGEQLGEGGMAIVYKAYDTRLETDVAVKVIR
                     TDNILPSVLDSALKRFEREAKLLARLLHPNIVKVIDYGEFEGMPYLVMPYLPGGTLKR
                     KLGKPTSWQESFKLLLPIAEALDYAHSQNMIHRDVKPSNILLTQRGQPMMADFGIAKI
                     LDLENTQELTGTSMMIGTPEYMAPEQVTAKTVDHRADIYALGVVLYEMVTGRQPFLAD
                     TPMAVLLKHNSEPLPHPRQFVPNLPDAVEKVLLKALAKHPDDRYQSMEEFGAVMKRLL
                     QEPPPQFEAGSTWGTSRTQTVQSANGNSGWKILKWGVVAGLFCLVAGAGLFFVFQRLK
                     NLPVNSAMVSTPVAATETPSPVVPPTETPTSAPTPLPARIEDEKGVPMVLIPSGPFLM
                     GSLQERVEQDFATCEKETNGQCELSWFTNETPQHLVRLDDYYIDMYEVTNARYAECVA
                     ADVCVAPSPTSSFTRDEYYGLPEFADYPVIHVSWADGRLYCDWRGGRLPTEAEWEKAA
                     RGPDGLIYPWGNEFDGTKVNFCDSNCPIDWANKDYNDYFGDTSPVGYYESGKSPYGVY
                     DMAGNVWEWISDWYQEDYYSISPSFNPLGPETGQFRALRGGSWSNVSSAMPSSMRSWD
                     VVPSLAQFGYYGFRCARNANE"
     gene            complement(6938..8962)
                     /locus_tag="PLE39_10075"
     CDS             complement(6938..8962)
                     /locus_tag="PLE39_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68718.1"
                     /translation="MKSRLVILTVLITLLFGIPQGISAQTSPGLLLPIGAGYTDTYTA
                     LGQYAVANAKGDVVHILVLASPYSTDAEHISEGERAQNLKDAEERRIQTEGACQRNLP
                     EGSTLTCKVELLPIFTQEDSNNPETLAYFTDDVSMIFILGGDQETGMGAIINTPVEER
                     INELHEMGTIIAGTSAGAAMQSKIMIASLNIGYATDDGLQAGALKLWDTEEKHGFSFS
                     IQNAVLDQHFFQRARMGRLVNVITQPGKPHVGVGVDAYTGVVSDNEVLRDVFGLYTVT
                     VLDAETYHSADAVKYVEFSETRPPLISVRNIILNLLSPGDVTYDLKTRTSSLGNPPDV
                     LERNFDALTLPAGAGPLFLGGDQLDTLKDSPILEKFKEVAGENILIVATGYPSSRSAE
                     TAIKKYTEGLGMQVQSLVVGDAPIVIPDDVTAVLVVGKDTSKVKVEALAPLKDFWLAG
                     NPVMADNAVTPVLGAFYSNHEPTPDDSELEELATQKSFWQGRTKMAPGLGFLKITIEP
                     QLLADLRFGRLFSLAYNHPEFIAIGVNKDTAIMLDENGATALGLNGIFVLDLRKAKLQ
                     LGTNDGFVINNGMLDIFAPGESIQAEIADVNARFDPAPTPALPTPVPTATATVAATPT
                     QPATATPSIVPTSPTAMEYAETQSPAPFPLWIIAVVVAIVSALVYFWKRK"
     gene            complement(9029..9952)
                     /locus_tag="PLE39_10080"
     CDS             complement(9029..9952)
                     /locus_tag="PLE39_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE68719.1"
                     /translation="MAATSPFSSERPKSLREQSLFMFYWARFRKHTMALIGGVVTLLV
                     LILIVFAPLISNYDPMTQDLLNRFQPPSSQHWMGTDDLGRDMFSRVMYGGRISLSVGM
                     LAMAIAIFLGSTIGLISGYYGGWVDSILMRMTEIFMSIPSLFVLIALGMFLRTLDLPS
                     LKAGSFLPISIVIGALSWMGTARLVRASTLELREREFVHACRALGGSDTRILFLRILP
                     NIASPIIVSATLGLAGAIISESGLSYLGFGVQLPTPTWGNMLSNTQSQMTTAPWTAIF
                     PGLMIFIVVIAINYLGDGLRDALDPRHSVKK"
     gene            complement(9955..10926)
                     /locus_tag="PLE39_10085"
     CDS             complement(9955..10926)
                     /locus_tag="PLE39_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE68720.1"
                     /translation="MAIYFTRRVLQSLFTLIVISMILYGLISSVPGGFMSAYTERSDF
                     TQEDLARIRAKFGLDDPIPVRYFKWLTAMLQGDMGNSFTTKRPVLQEIADRLPNTLLL
                     MGIMMVTTLLVAIPLGIISAVKQYTWFDHIMTTVAFAGQSLPVFWFGLLLIIIFAVTL
                     KGADGKPLLPGSGMYTLGEPFSIADRIKHLILPISMLTFVSAAGYMRYLRSSMLDVIH
                     EDYVRTARAKGLKERLVIYRHALRNAIIPLVTLVALDLPSLFGGALFTETIFAWPGIG
                     RLYFTAALKTDYPLVMAVLMIESALIIGSNLLVDFIYAALDPRIRLS"
     gene            complement(11112..12860)
                     /locus_tag="PLE39_10090"
     CDS             complement(11112..12860)
                     /locus_tag="PLE39_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE68721.1"
                     /translation="MKFLKNVLVLMVVASFVLSACGSPAAEQPAETDAPAATDAPAAT
                     EAPAATEAPAVDPNAPVSGGTVIIGTPQEPGMMNTLLTSSSIEDAVVSLFGEGLVSTN
                     ENGEYVPVLAKELPTVSEDGLVVTWLLKEGIKWSDGSDFNCDDVRFTMEGALSDLSQV
                     SASGYRDIETLECPDPYTVVATFGEVYAPYLRLFSYTVPDTAGALEDMETWDINRNPV
                     GTGPFILTEWEPGDHLTFSRNPLYREAGKPYLDNVIIKILPSREVGMQLLGTGEIHAL
                     WDLTEANFPELEQLSSQGVSFVSTVTGENELLSLNFGANDGTAPADPATAPHPILFDL
                     KVREALQLAIDKQTIVDTLLYGNVKAGNSAVPVGTFGCPQPVSEFNLEKANALLDEAG
                     WVVGADGIREKDGVRMSLKIATTTGNQLREQTEQVLAEMLKAAGIELVIENVPSDVLF
                     ASWESDGMRDHGTYDIVMYTTGPSTDPDSHLYSSFHSSEIPTADNEGSGANFARYINA
                     DVDSWLDEAATITDTARRKELYCQVVEQVNKDLPRIYLYERLSIMGHRNEFHGLKISP
                     TFVDFAWDAANWWLSQ"
     gene            complement(12889..13704)
                     /locus_tag="PLE39_10095"
     CDS             complement(12889..13704)
                     /locus_tag="PLE39_10095"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNE68722.1"
                     /translation="MTTTTLMPMGGALDERKDPTVLQEFLRRAGGKKARIVILPQASA
                     RRDTGKEYIELFKAFGAKEVVSFEFRKRSEAGTAEQIKLIRKASGIFFSGGTQMRIAA
                     AMGGTALEAEIHAAYHRGCIVGGTSAGASILSKTMIAYGNGGPTPRERIIQFSAGLGF
                     TDKFIFDQHFRQRDRLGRLIYAVSSHPGVIGVGIDEDTAAIVEDNARLTVAGSGAVTI
                     VDGFQIADTDVAEIEHGGAVAVSNLLVHVLTHNSVFDGRTRKAALPKKKLLID"
     gene            complement(13701..14378)
                     /locus_tag="PLE39_10100"
     CDS             complement(13701..14378)
                     /locus_tag="PLE39_10100"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNE68723.1"
                     /translation="MLEELPSHRQLRKLVADQIRAAIFDGRFKPGEWLRQERVAQELG
                     VSQMPVREAMKELAAEGLIEHVPYRGVRVVALSMEDIEDLYEHRAFLEGRAAEKAVEY
                     ITDGEIMDLKLMVAEMESLPPEQIAQYRKVNRKFHETIFLISRREYLIRTLTQMWEAF
                     PTMLIGNFAVTAQQPLPQRDMQDQLEHRAIMDALEKHDGKKAGDAMKDHILSAARNLI
                     ESLKVSS"
     gene            complement(14513..15319)
                     /locus_tag="PLE39_10105"
     CDS             complement(14513..15319)
                     /locus_tag="PLE39_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68724.1"
                     /translation="MAAFKTFVKNMLLLLPNGERIVAWIVEKRRKPQPAPVKTPAPAV
                     VEPPVENKPADPRSVFTDYYFNNFWGNKESVSGAGSTMEYTENVRREIPLLMNRFGVK
                     KFLDAPCGDYNWFQAVQRPAGMEYIGGDIVEELIKQNQLKYGDASTRFISLDVITEPL
                     PSSEFWMCRDCLFHFSYDDIFKTLANFVRSDIQYLFTSIHTECTANADIVTGGARQLN
                     LELPPFNLCKPVLYVDDWIPGFTVRRMGVWTRQMVAESLASNAEAKKYLA"
     gene            15398..16546
                     /locus_tag="PLE39_10110"
     CDS             15398..16546
                     /locus_tag="PLE39_10110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE68725.1"
                     /translation="MSKHDVLIIGGGPSGLSTALHLKKIDPHLASRILILEKEQYPRF
                     KLCAGGLVIDAEIILQRLGLDVTEVPHVNVDSIHFDFMGSGLKVHVPNRHALRVIRRN
                     EFDAWLAKKTLEAGIEIREGVAVMNVTPTDDGVTVETDQGVFFAQVVIGADGSNGITR
                     RCILPNAPISTARVLEVLTPAHPQVSRGREKDAFFDFFPVPNNIAGYVWDFPTLVNGE
                     TMRCWGVYDTNLLADEKRPALKEPLAREMARHGFDLNQFELKGHPIRWYAPQNQISVP
                     RVLLVGDAAGADAIFGEGISMALGYGAIAAQEVSESFRRSEFSFRRYRHRFARSGLGQ
                     TLFVRWVITNIIYPIKWKWFQILLWRIAKPIVLIIAWVFVLNWSKRIK"
     gene            16543..17655
                     /locus_tag="PLE39_10115"
     CDS             16543..17655
                     /locus_tag="PLE39_10115"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNE68726.1"
                     /translation="MIPGLDGLRAVAFLIVFFFHTRNLPFGWMGVQLFFVLSGFLITD
                     ILLRMKEKLPQREFFIKFYGRRFLRIFPLYYFYLILLAGLVFVLPPLNIGLLNAELGK
                     SFINQIWVAAFYLYDFFHASVFFERSRFFTHLWSLSVEEQFYLLWPLLIFLVPRQKFK
                     QLCLTAIGLGFVFRLVITLVYRAGIFPFLLNDPQQAVNVLPFSHLDAFAFGAYISRFE
                     IPRPRLQLGLLAIFVPALGLLTDFLSKGSVVVASLGYDLPMTGFYKEVWGYTLLNYLF
                     AVLIHCVARTELLNSILESAPLRYLGKISYGLYVYHYGIIALAVAVFREYGLPYSVRS
                     PQMFVAAMGSTLLIATLSFYLLEKPIIDLKDKFFRI"
     gene            17722..>18486
                     /locus_tag="PLE39_10120"
     CDS             17722..>18486
                     /locus_tag="PLE39_10120"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNE68727.1"
                     /translation="MGTADLHIHSIYSHDATTTVRAILKQAADVGLDVIAVTDHDEIR
                     GSLEACELAPKYGVEVIPGAEVTTKDGHLVALFVETLPPAGLPLVDTLIHIGKQGGIA
                     IAPHPFNNLPGSLSMESVVSALTNPRAKSVLKGMETHNMGTQSFDSVAQKLSIYLPLA
                     KIASSDAHIYWAVGAGRTEFPGKTAQDLRTALEKNTTVPIPYHEEFSAKAILSWVRRV
                     TLRRFGYASDSISATQPINTQKLSESFIQKTKKKIIK"
     assembly_gap    18487..18586
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18608..19366)
                     /gene="recO"
                     /locus_tag="PLE39_10125"
     CDS             complement(18608..19366)
                     /gene="recO"
                     /locus_tag="PLE39_10125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNE68728.1"
                     /translation="MTEFRSFRAFAVVVRHTDWGEADRLLTLYTREQGMVKALAKGAR
                     KINSRKAGHLQPFTHVTVQLAKGRDLLIVTQAETVDAFLPLHDNLVKTGYAAYIVELL
                     YRFSYEEEGGNPSLFKLLVDTLGRIEKEDEAWLPVRYYEMRLLDIVGFRPQLFECANC
                     GREILPEDQYFSFVTGGVICPQCGLGLPNLAKISMEALKYFRHFQRSSYRDASRAHPS
                     FEVQKEAEALMQGYFTYLLERELNTPGFIKRVKS"
     gene            complement(19421..19825)
                     /gene="cdd"
                     /locus_tag="PLE39_10130"
     CDS             complement(19421..19825)
                     /gene="cdd"
                     /locus_tag="PLE39_10130"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNE68729.1"
                     /translation="MDLTNDERQTLIKQAVEIRKRAYVPYSNYPVGAAVRTKSGKVFT
                     GVNIENAAYPQTMCAERVAIFKAVSEGESEFDVITVVTNNGGSPCGGCRQVMAEFGLD
                     TVVLIADGDGNLHKELTVADLLPEAFTPKHLQ"
     gene            complement(19853..21214)
                     /gene="glmU"
                     /locus_tag="PLE39_10135"
     CDS             complement(19853..21214)
                     /gene="glmU"
                     /locus_tag="PLE39_10135"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNE68730.1"
                     /translation="MKVTAVLLAAGQGTRMKSSLPKVLHPIAGRPLIWHAMQAISQST
                     TEKPIVVVGHGAEEVTKYLGDSAQTVLQEPQLGTGHAVMQAEDLLRGKTDLVVVCYSD
                     MPLLRGETLQALVETQKKNKGPISMLTVHADDPRGFGRIIRKADGTVDAIVEEYVATP
                     EQLQVKELNVGGYCFDANWLWDALHRIPKNPKKGEYYLTDTVELASQAGLPVQATVMD
                     DVEETIGINTRLHLADAEAAMRRRINQQHMLNGVTIIDPASTYIEAGVSIGRDTVIMP
                     NTYLHGRTEIGEGNVIGPNTIIHDSKIGNHCKVLASVMEGALLDDHVDMGPFARLRKG
                     AHLGNHVHMGNFGEVKDSYLADGVKMGHFSYIGNAQIGSNTNIGAGTITCNYDGEKKH
                     PTEIGEDVFIGSDTMLVAPLKIGAGARTGAGAVVTKNVSEDTLVVGMPARAIKKLERK
                     TKK"
     gene            complement(21355..21428)
                     /locus_tag="PLE39_10140"
     tRNA            complement(21355..21428)
                     /locus_tag="PLE39_10140"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21394..21396),aa:Gln,seq:ttg)
     gene            complement(21457..22557)
                     /locus_tag="PLE39_10145"
     CDS             complement(21457..22557)
                     /locus_tag="PLE39_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE68731.1"
                     /translation="MDRNILLVDDQRDILRLLRSTLETLKKTEIKIFEAPSGEEALIE
                     SSRHKVDLLVTDYKLPGMSGLELMHKVRVRHPEAKVILITGLTDRKVREEMLNAGALA
                     VFDKPIPLADFLDVVERGLGFVQTIFPPERPSDEKTERRHARVSDLLANFRQDINAEA
                     VFLLNERGLVQARTGKLRDESMEVSLFSTLMAIHNASLKVAKYNHQEKLDSYHVFSSG
                     DNDLLFIPVAPMYALLVAGNGLVREDRILESVNALLALRKEVERSLRSLGVTGELQSI
                     SPKTVPLATAAAPTIPIPASIKPTEKPAEAAPSPELEALLKNAAATKDQTANMDDFWN
                     QAAEKHGNKPASKEVISLEDARKLGLLPGDGK"
     gene            complement(22564..23556)
                     /locus_tag="PLE39_10150"
     CDS             complement(22564..23556)
                     /locus_tag="PLE39_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68732.1"
                     /translation="MSQTTEMQKTEDLVQVEHLVKYFPVRAGLMQRVVNWVKAVDDVS
                     FTVKKGETLGMVGESGCGKTTIGRSMLRLVEPTSGSVQYDGRDVLKLRGSELKDVRRH
                     MQIIFQDPYASLDPRVPIGESVMEGLHIHKIGSPQERYDLMIETLKKVGLEDYHARRY
                     PHEFSGGQRQRIGIARALALRPNFIICDEPVSALDVSIQSQVLNILKDLQKEFGLTYL
                     FIAHNLSVVEHISDRVAVMYLGKMVELTSRDDLFKNPLHPYTKALMSAIPIPDPKLKR
                     QREVLKGDVPSPLNPPKGCRFHPRCPIADKICSEQEPEFREAAAGHFVACWMVK"
     gene            complement(23622..24653)
                     /locus_tag="PLE39_10155"
     CDS             complement(23622..24653)
                     /locus_tag="PLE39_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68733.1"
                     /translation="MTKTDRKPLLEVRNLKTYFYTEDGVVSAVDGVSFEVYPGEVLGI
                     VGESGCGKSVTSLSIMRLIAPPGKITEGEILLDGKNLLNLSEDEMTKMRGNKISMIFQ
                     QPQTALNPVFKVDNQIAEVLNIHKAVGKEAGRQRAIELLKMVGIPDAERRADAYPHEL
                     SGGMAQRVMIAMALACTPELLIADEPTTALDVTIQAQILDLMRDLRTKMGTSVILITH
                     DLGVVAEMAERVAVMYAGEVVEQAEVNSLFEQPLHPYTQGLIGSIPVLGQIKERLDVI
                     PGSVPNLVNLPPGCRFAPRCTAREKFGLKICTEVKPELTDAAPGHKVRCWLYQNAKDH
                     QAPLKAKQG"
BASE COUNT         5963 a         6491 c         6293 g         5805 t
ORIGIN      
        1 acacggatca actcccccat cacacgttcc ttgatatagg gtttcaaact gtcgggagtc
       61 ccaaggtcaa cggggcagcc gagtaatttt tcaagataat cctgcaaagc aaacagggcg
      121 gttgaactcc acaagcaagt ccacatcact ggtggacgtg gcatcatccc tggccactga
      181 accaaacaac tgcaaggatt tcacaccaaa cttagtgatc aattcggcat ttttttgttt
      241 cagtgtttga agaacaactt cctgtttcat attgcaatta taactcgcaa aactgactgt
      301 attgtcaact tggcgagagc gtaaatgaac agccagtcca ctgcggactg gctgtttttc
      361 ctgttgtata atttgaatat gacacgcagg acagtgatta cccgtatccg caagcaccgc
      421 gcccaattga caaaactggg cgtaaagtca ttgtccttat ttgggtcggt ggcacgcgga
      481 gaggaaggtc caaacagcga cgtggacatc cttgtggaat tcaaggggaa agcgacactt
      541 gaccgttaca tggacacgaa gttctatctg gaagacttgc tcggctgcaa agtggatctg
      601 gtcaccccga aggcgatcaa gccacgtatg aaaccctata tcatgcagga cctagtccat
      661 gtcacgggat gaatcacttt atctggcaga tgttcaagaa tcttgtgaaa aggttctgag
      721 attcaccaag gggatgacct ataaagactt tgtcaacgac gaccttcatt tcgacgcagt
      781 gttacgcaat ctggaaatca tcggggaagc agtgaagaat atttccgagg caacacggca
      841 gaaatacccc gatgtgaaat ggcgaaaaat cgctggcttc cgtgacattg tggcgcatga
      901 atatttcggc atcaatgatg aaacagtttg ggacatagtg gaaaatgaag tccctgcttt
      961 attggcagtc atgaaaataa tgcttgagga aaagtaacac ttaaaaaatt acaagcacat
     1021 aagaaaaaca gccagtccac tgcagactgg ctgtttgcat ctgacaacat ccacctttac
     1081 ggcggtgaaa acacattgat gatgccgacg aacaccatca cgtatccaaa aatgcccaga
     1141 gattcaaaga agccgatcac tccaagcacg atggcggcaa tcgccaaatt gcgattgatc
     1201 tcacctgcac gcagtgaaag gattcccaaa atggcgccga tcaggggcag gagtgcggca
     1261 aggcacatca acgcctgcgc aatgtaccag ctgggtttgc cttccataaa agcatcgaac
     1321 ccattcgggt tgttgaccag aacggagatg cagcagaaga aaaacgagga aagcgaaagc
     1381 agcagggcag cgatcgggcg gttcgatctt ttttgagggt tgggttgaac gggggaagac
     1441 gcatccatga aaattctcac ttgtaatttt tattgattca catcgcttgc acagcgaaag
     1501 ccaaaactca aaaagacatc ggtggggaca accccaagac gggcgctggt gcgaagcaaa
     1561 tattcgtgtt gattccaacc gccgccgcgc agcacacggc gctccccgct ctcggcccca
     1621 accgggttat ctgtctctga aacatcgtat gcgggcgaat accaatcggc cacccactcc
     1681 caaacattgc ccgccatgtt ataggcgccg tacgggctga ggctgcccgc ataggaatcg
     1741 aaaggctggg tatcttcaat acaactcagg taattcgcct gactgcaagc gatcccctca
     1801 ccccacggat aggtacgtgc gtccattccg cgcgcggctt tttcccattg cgcctcggtg
     1861 ggcagttgcg catcgcgcca atcgcaatag gtcctcgcca tctcccaatc cacatacacc
     1921 acgggatgat tctcatactc ggggttgttg tagttcgtca tatcctgtgg aggtttgcag
     1981 accccatcga ccacacagac tctgtaatct aaattggtga cctcatattt atcaatatag
     2041 aattcatcca gatacaccgt gtggacgggc cgctcatctt catcaccgtt atcactgccc
     2101 attacaaaat caccttcagg cacaagcacc atcaccgcat ttttagaatc ggtgatctcc
     2161 gctgccaatg gggtggattt cttcttcatc aacactactg ccgctgcgac gacgacggcc
     2221 aacaggatga ctccacccaa agtggcggcc ttccaattaa acgggaccag ttttcggctc
     2281 ggagaagacg cgtctttcct aaccgacgac tggcccggcg atcctttgag agtctccatc
     2341 ctgttaatgg gtaccttcgt ttccgcattg ctcaacaaca gttccatgtc tctaatgaat
     2401 tcggtcatgc tggcatggcg gttggaaggt tcgcgagaca aagccttgag caaaaactgc
     2461 tccaccgctt caggcaggtg cggcacaata tcacgcggaa aaagcacagg ttcggtcact
     2521 tgcatcataa aaacgccgcc aggtgtatcg gctgtgtagg gcctgcgccc ggtgaccatt
     2581 tcgtacagaa cgactcccag ggcatacata tccgattgcg gcacggtctt tcccgtccat
     2641 tgttcgggcg ccatgtattc gggtgttccc atggccatgc ccgaagcggt gagcagcgtg
     2701 ccctcgtttt ggaataactt ggcgatccca aaatcagaca gggtgggtgt ttcgttttcg
     2761 gcaagcagaa tgttcgaggg tttgatatcg cggtgaataa tctcgcggtt gtgggcgtat
     2821 tcaactccgc gcgcaacagg caacagtaaa tgcatggcat cctgccacgc aatgggtgaa
     2881 ccgatctttt gtttgagcgt gccgcccggg agatattcca ttacaaggaa gggcgaacct
     2941 tcatattccc catagtccaa caccttgaca atgttcgggt gtgaaagccg cgccagagat
     3001 ttcgcctcac gttcgaaccg tttgagttca ttctgcaata cgtcgggcgg aaaggcttca
     3061 tgccgaatga ccttgagcgc cacttcacgt tcgaggcggg tgtcgtaggc tctatacacg
     3121 gtggccatgc cgccctgtcc caacaggtcg ataatattgt aacggtcaat gtgttttccg
     3181 ataaaatttt ccattccgtc tgtcacatcc gatcacatta aagaatgagc caccgcgaaa
     3241 ttatacgtca ttccccccgc actagggcgc atccgccgcg cagcggaacc cgaccctgct
     3301 aaaaaaatct tcgggaaaat atgccccgcg tgccgagacc cgtttaaggc gttcagtggc
     3361 atcaaatgca ccaccacgca agacatgaga ttcgccactc gaagggccct gcgggttttc
     3421 aagcggggaa atggaatagt attcccgcga ataccaatcg gccacccact cccaaacatt
     3481 tcccgccata ttgtacacac cataggggct ttttccctct ccaaaactgc cgacttccaa
     3541 tgtgtcagcg gtgcaattgc tttttgcaca gtcgggcgca tcaccccagg gataaatgcg
     3601 attatcggtg ccacgtgcgg ctttttccca ttctgcttca gtgggcaggc ggccgccgcg
     3661 ccaggcgcag aaagtactgg ccctatccca atctatatta atcactggga aattctcata
     3721 ctcagagaca ccatagtact cttcccttgt aagtgacccg gtggagatgg gtggtttgca
     3781 gattgaagcg tccacacatg cctgataggc agcattggtt acttcatata catcaataga
     3841 ataggcatcc aatctcacca agtggatggg aagttcatct gcatcaccgc cttcactgcc
     3901 catattgaaa tcacctgcag gaatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnggagtg taaccgtgac agtgcctgcc tgcatttttt gttgaaggga tgcgatcttc
     4081 tcttctacac caaaataggc agggtcaatg cgattcacct gcctgtaatt ctctatggcc
     4141 gccatccaat cggcgtcctt ttccatcttc tccgcttgct ggaccaggaa aacgacctgc
     4201 ctcctctttt ccgctttggc atcgaaccgc atcagggaac gcgcattggc ccggatgcga
     4261 tcactacgaa ggacaagggc gcgcaaaagt ttttcataac cgttctcttc gaacagatcc
     4321 acccattgcc atttactgag gcttgccaga ctctcgcatt cctccaaacg ggcagggatg
     4381 ataaagatct ccccttcggg tttttccatg gcggtatcca gtgcaagccg cacttctttt
     4441 tgacggaacc ccgcctgatt gaattgattt gaaaggcaga tcaccactac atcagattcc
     4501 cgcaccgccc tgcgaatttc caattcccac tcctgcccag gcagaagttt ttccttatca
     4561 agccatgcat ccacgccgtc taaaaccagg cgggaataca aagcacgtac ggcagtgcgg
     4621 tctgaatgag aatgacaaag aaatacctta aggggtcgtg gagtttggga catgggaggg
     4681 aagttaaggt caataaagta gtgactggat tgtaaaatgc ggcctgataa atttaatacc
     4741 cgatttactc gttcgcgtta cgggcacagc gaaagccata ataaccgaat tgggccagcg
     4801 agggcaccac atcccaactg cgcatggagg agggcatggc actggatacg ttgctccatg
     4861 aaccgccgcg caaggcacgg aactgacccg tttccgggcc cagaggattg aacgatggag
     4921 agatgctgta atagtcctcc tgataccagt cactgatcca ttcccaaacg ttgcccgcca
     4981 tatcgtacac accatatggg cttttgccgc tttcgtaata gccaacaggt gaggtatccc
     5041 caaagtagtc gttgtaatcc ttgtttgccc aatcgatcgg acaattggaa tcgcagaaat
     5101 tcaccttggt accgtcgaat tcattgcccc acgggtagat caaaccatcc gggccgcgtg
     5161 cggctttttc ccattctgct tcggtgggca ggcggccgcc gcgccaatca caataaagcc
     5221 tgccatccgc ccaactgaca tggatcacag gataatccgc aaattccggc aggccgtaat
     5281 attcatccct tgtaaatgaa ctggtcggcg atggcgcaac gcagacatcc gcggctacac
     5341 actcggcgta gcgcgcattt gtcacctcgt acatgtcaat gtaataatca tcgagccgca
     5401 ccaaatgttg aggcgtctca tttgtgaacc acgataattc acactggccg ttggtctcct
     5461 tctcgcaggt tgcaaagtcc tgttccaccc gttcctgcag cgaccccatc aaaaacggcc
     5521 ctgacgggat caataccatg ggaacaccct tttcatcttc aatgcgggcg ggcaatgggg
     5581 tcggcgcact tgtaggtgtt tcagtaggcg gcacaacggg agatggcgtc tcagtcgccg
     5641 ccacaggtgt tgaaaccatg gcagagttca caggcagatt cttcaaacgt tgaaacacaa
     5701 aaaagagacc tgctcctgca acaagacaaa acaagcctgc aacaactccc cacttcaaga
     5761 ttttccaacc tgaatttccg ttggcggatt ggactgtctg cgttctggat gtaccccagg
     5821 ttgatcctgc ctcgaactgc ggaggtggct cttgaagcag ccgcttcatc acagcgccaa
     5881 attcctccat gctttgatag cggtcatcgg gatgttttgc cagtgctttg agcaacacct
     5941 tttccactgc gtcaggtaaa ttcggcacaa actggcgcgg gtgcggaagg ggctcgctat
     6001 tgtgtttgag cagcacggcc atcggggtat ctgccaaaaa aggttgacgg cccgtcacca
     6061 tttcatagag gacgacacca agagcataga tatcggcacg atggtcaacg gttttggcgg
     6121 tgacctgttc gggggccatg tattccggcg taccgatcat catgcttgtg cctgtcaact
     6181 cctgtgtatt ttcgaggtcg aggatcttgg caatgccgaa atcggccatc atgggctgtc
     6241 cgcgttgggt gagcaggatg ttggacggct tcacatcacg atggatcatg ttttgactgt
     6301 gggcataatc caaagcttcg gcgatcggca gcagcaattt gaacgattcc tgccaggagg
     6361 tgggcttgcc caattttctt ttcagggtgc cgccgggcaa ataaggcatt accagatatg
     6421 gcataccttc aaattcccca taatcaatga ccttgacgat attcgggtgg agcagacgtg
     6481 ccagcaactt ggcttcacgt tcaaaacgtt tgagggcact atccagcacg ctgggcagaa
     6541 tattatctgt gcggatgact ttgacagcca catcagtttc gaggcgggta tcgtaggctt
     6601 tatagacgat ggccatgccg ccttcgccca gctgttcacc gatgtgataa cgtcccaaag
     6661 actttccaac cagatcaggc ataaccacct ctatgcgaat caaaaccaca tttcacaacc
     6721 atgcctgcat gagcatttct tcagccgtat ccctcccatg cctttgtgat gtttaaatta
     6781 taagtcagtt tccgcttttg gttcattggg aatcgctgtg gcacagcgta agtcgaattt
     6841 gcaattcgac ttacgtgatt caccttttcc atatccagca aaatataaaa aaacagccaa
     6901 cctttgtcgg gttggctgtt tgagaatcgg gttggttcta tttacgcttc cagaaatata
     6961 ccagggcgga gacgatggcc accaccactg caatgatcca caacgggaag ggggcggggc
     7021 tttgggtttc ggcatactcc attgcggtgg ggctggtcgg cacgatggag ggggtggcgg
     7081 ttgcaggctg ggtgggtgtg gcggcaactg ttgcggtggc tgtcggcacc ggggttggca
     7141 gggcaggcgt cggcgcggga tcgaagcggg cgttgacgtc ggcaatttcc gcttgaatgg
     7201 attcgcccgg ggcgaagata tccagcattc cattgttgat cacaaagccg tcgtttgtgc
     7261 caagctgtag ttttgccttg cgaagatcga ggacgaaaat accgttgagg ccgagagcag
     7321 tggcgccgtt ctcatcaagc atgatggcgg tatctttgtt gacaccgatg gcgatgaact
     7381 cagggtggtt gtaggccagt gagaacaggc ggccaaaacg caggtcagcc agcaactgcg
     7441 gttcaatggt gatcttgagg aagccaaggc cgggcgccat tttggtgcgt ccctgccaga
     7501 aggatttttg tgtggcaagc tcttccagtt ccgaatcatc gggggtgggt tcgtggttgg
     7561 aataaaacgc accgaggacg ggcgtgaccg cgttatccgc catgaccgga ttacccgcca
     7621 gccagaaatc cttgagcggt gcaagcgctt ccaccttgac cttggatgtg tccttgccta
     7681 cgaccaacac ggcggtgaca tcatcaggga taacgatggg ggcatcacca accaccagcg
     7741 attgaacctg catgccaagg ccctcggtat attttttgat ggcggtttct gcagagcgtg
     7801 atgatggata ccccgtggca acaatgagga tattttcacc cgccacttcc ttgaattttt
     7861 cgagaatggg gctgtctttg agcgtatcga gttgatcgcc gccgaggaag agcggacctg
     7921 caccagcggg gagggtcagg gcatcaaaat tgcgttcaag cacatcaggc ggattgccca
     7981 gtgatgaagt gcgggtcttg aggtcgtagg tgacatcacc cggggaaagc aggttcaaga
     8041 tgatattgcg aacactgatg agcggcgggc gggtttcgct gaattcgaca tatttcacgg
     8101 cgtcggctga gtgataggtt tccgcatcca aaacagtgac ggtgtacagg ccgaaaacat
     8161 cgcgcaagac ttcattgtct gaaacgacac cggtataggc atcaacaccc acgccgacat
     8221 gaggcttgcc gggctgggtg atgacattga caaggcgtcc catgcgggca cgttgaaaga
     8281 aatgctggtc gaggacggcg ttttgaatgc tgaaggaaaa accgtgcttt tcttcggtat
     8341 cccagagttt caatgcgcct gcctgaagtc catcatcggt ggcataaccg atattgagcg
     8401 aggcgatcat gatcttcgat tgcattgccg ccccggcgct tgtgcccgca ataatggttc
     8461 ccatttcatg cagttcgttg atgcgctctt cgacgggggt attgatgatg gcccccatgc
     8521 cggtctcctg gtcgccgcca aggatgaaga tcatcgaaac atcatcggtg aaataggcga
     8581 gggtttcagg gttgttggag tcttcctggg tgaaaatggg caggagctct actttgcagg
     8641 tgagtgtgga gccttcggga aggttgcgct gacaggcgcc ttcggtctgg atgcggcgtt
     8701 cttcagcatc tttcaggttt tgggcgcgtt caccttcgga aatatgttcc gcatcggtgg
     8761 agtagggaga ggcaagtacg aggatgtgaa ccacgtcccc tttggcgttg gcgacggcgt
     8821 attgcccgag ggcggtatag gtgtcggtgt agccagcgcc gatgggcaga agcaggccgg
     8881 gggaagtttg agccgagatt ccctgcggaa ttccaaacag cagggtgatt aaaacagtca
     8941 aaataacgag gcgtgatttc attttcagtc tctcccaaaa tcgtggatca caaagcgcag
     9001 caggcttata ctcaaaaatt gccgcggtct atttctttac agaatggcgg gggtcaaggg
     9061 catcgcgcaa cccatcaccg agatagttga tggcgatgac aacgatgaag atcatcaggc
     9121 cggggaagat ggcggtccac ggagcggtgg tcatctggct ttgagtgttt gaaagcatgt
     9181 tgccccaagt gggtgttgga agttgaacac caaagccgag atatgaaaga cccgattcgc
     9241 tgatgatggc gccagccaaa cccagggttg cagaaacgat gatggggctg gcaatgttcg
     9301 gcagaatccg caaaaagagg atgcgcgtat cggaaccgcc caaagcacgg caggcgtgta
     9361 cgaattcgcg ttcgcgcagt tcaagcgtgg aagcacggac aaggcgggcg gttcccatcc
     9421 atgaaagcgc gccgatgacg atcgagatcg gcaggaagga acccgctttc aaactgggca
     9481 ggtcgagtgt gcgcaggaac atgcccaatg caatgagcac gaacaaactg ggaatggaca
     9541 tgaagatttc ggtcatacgc atgaggatgc tatccaccca gccgccgtaa taaccggaga
     9601 tcagcccgat ggtggaaccg agaaaaattg caatggccat ggcaagcatg cccacactta
     9661 aagagatgcg gccgccatac atgacacgtg agaacatgtc acgcccaaga tcgtcggtgc
     9721 ccatccagtg ctggctggag ggcggttgaa agcgattgag cagatcctgc gtcatcgggt
     9781 cgtaattgga gatcagtgga gcgaacacga tcagaatgag gacaaggagc gttaccacgc
     9841 caccgatcaa agccatggtg tgcttgcgga aacgagccca atagaacatg aacagggatt
     9901 gttcacgcag ggattttgga cgttcggagg aaaaagggga agtggctgcc atggttacga
     9961 taaccgaatg cgcgggtcaa gagccgcata aatgaagtca acaagcaggt tcgagccgat
    10021 gatgagagct gattcgatca tcagcaccgc catcaccaac ggataatcgg tcttgagggc
    10081 ggcagtgaaa tacaagcgcc cgatgcccgg ccaggcaaag atggtctcag tgaataacgc
    10141 gccgccaaat aatgaaggca ggtcgagtgc caccaacgtc accagcggaa taatggcatt
    10201 gcgcagggcg tggcggtaaa tgaccaggcg ttccttcaaa cctttggcac gcgccgtacg
    10261 gacgtaatct tcatgaatga catcaagcat ggaagaacgc aggtaacgca tataccccgc
    10321 tgcggaaaca aaggtcagca tggagatcgg cagaatgagg tgtttgatgc ggtcggcaat
    10381 cgaaaagggt tcacccagcg tatacatgcc cgaccccggc agaagcggtt tgccatctgc
    10441 acctttcaag gtgacggcaa atataatgat gagcaaaagt ccgaaccaga atacaggcag
    10501 ggattgcccc gcaaaggcaa ccgtggtcat gatatggtca aaccaggtgt attgcttcac
    10561 cgctgaaata atacccaatg gaatggcaac cagcaaagtg gtcaccatca tgatgcccat
    10621 cagaagcaag gtgttcggca ggcggtctgc gatttcttga agcacggggc gtttcgtggt
    10681 gaacgaattt cccatgtcac cctgaagcat ggcagttaac catttgaaat agcggacagg
    10741 aatggggtca tcaaggccaa atttcgcacg aatacgcgcc aggtcttcct gtgtaaagtc
    10801 agagcgttct gtataggcgc tcatgaatcc gccgggcacg gaggatatca ggccatacaa
    10861 aatcatactg atgacgatca atgtaaataa tgattgcaga acacgacggg tgaaataaat
    10921 ggccataatg atgactttac ccagattgct gttttatcca aaaagacccg cggccttttt
    10981 ggtggatgga tgcatccatc cattgagatg ccgaaattat actttgacta aaaaactcaa
    11041 gtgaagatcg aagatttcaa aaggattgcc cctcctccca tgataagagg aggggcaaaa
    11101 tacactttag attactggct caaccaccaa ttggcggcat cccaggcgaa atccacgaag
    11161 gtgggggaaa tcttcaaacc gtggaattca ttgcggtgtc ccatgatgga gagacgttca
    11221 tacagataga tgcggggcag gtctttgttg acctgttcaa cgacctggca atacagttcc
    11281 ttgcggcggg cggtatcggt gatggtggca gcctcatcaa gccaggaatc gacatcggca
    11341 ttgatgtagc gggcgaagtt ggcaccggaa ccttcattgt cagcggtggg gatttcagag
    11401 ctgtggaagc tggagtacag gtggctatcc gggtcggtgg aaggaccggt ggtgtacatc
    11461 acgatatcgt aggtgccgtg atcgcgcata ccatcgctct cccagcttgc gaacaacaca
    11521 tcggagggaa cgttttcaat gacgagttca atgccagcgg ccttgagcat ttcagccagt
    11581 acctgctcgg tctgttcgcg gagttggttg ccggtggtgg tggcgatctt caagctcatg
    11641 cgaacgccat ccttctcacg gatgccatca gcgccaacaa cccagccagc ctcatccagg
    11701 agggcattgg ccttttcaag gttgaattcg ctgacaggct gcgggcagcc gaaggtaccc
    11761 acgggtacgg cgctgttacc agccttcaca ttgccataaa gcagggtatc cacgatcgtc
    11821 tgcttgtcaa tggcaagctg caacgcttca cgcaccttca aatcgaacag gatcgggtgg
    11881 ggagctgtgg cggggtcagc gggggcagta ccgtcattgg caccgaagtt gagggagagc
    11941 agttcatttt caccggtcac ggtggagacg aacgaaacac cctgtgaaga aagctgttca
    12001 agttcgggga agttggcttc ggtcagatcc cacagtgcgt ggatttcgcc cgtgccgagc
    12061 aactgcatac ccacttcgcg ggaaggcagg atcttgataa tgacgttatc gaggtagggc
    12121 ttgcctgctt cacgatacag cggattgcgg ctgaaggtaa ggtgatcgcc aggttcccat
    12181 tcggtcaaaa tgaacggacc ggtaccaacc gggttgcggt tgatatccca ggtttccatg
    12241 tcttccaagg caccggcggt atcgggcacg gtgtaagaga acaagcgcag gtagggggcg
    12301 tacacttcac cgaaggtcgc aaccacggtg tagggatcgg ggcattcgag ggtttcgata
    12361 tcgcgatagc ctgaagcgct cacctgagaa agatcggaaa gggcaccttc catggtaaag
    12421 cgcacatcat cgcagttgaa atcggaacca tcagaccact taatgccttc cttcaaaagc
    12481 caggttacaa cgaggccatc ttcagaaacg gtcggcaatt ccttggccaa cacaggaaca
    12541 tactcgccat tttcgttggt ggagacaaga ccctcaccaa acaaggacac aactgcatcc
    12601 tcaatggaag aggaggtcaa cagcgtgttc atcatgccag gttcctgcgg tgtaccgata
    12661 atcacagtac caccactgac gggagcattc ggatcaacag caggggcttc ggtcgcggcg
    12721 ggggcttcag tcgcggccgg ggcatcggtt gcagcagggg cgtcggtttc agcaggctgc
    12781 tcagcggcag ggctgccaca agcagagagc acgaacgatg caacaaccat caaaaccaaa
    12841 acgttcttca aaaacttcat aatcttctcc ttttttgatc tatgctccct aatcgatcag
    12901 gagctttttt ttcgggaggg ccgcttttcg cgtccgcccg tcgaacacac tgttatgggt
    12961 caacacatgc acaagcagat tggaaaccgc caccgcgccg ccatgttcga tctcggccac
    13021 atcagtatcg gcgatttgaa acccgtccac gatcgtgacc gcacccgaac ctgccactgt
    13081 caaacgggca ttatcttcca cgatggcggc ggtgtcctcg tcaatcccca caccgatcac
    13141 accggggtgt gaagatacgg catagatcag gcgacccaat cggtcgcgct gacggaagtg
    13201 ctggtcgaag atgaatttat cggtgaaacc aagccccgcc gaaaactgga tgatccgctc
    13261 acggggagtc ggaccgccat ttccgtaggc gatcatggtc tttgaaagaa tggatgcccc
    13321 ggcgctggtc ccgccaacga tacatccgcg gtgataagct gcatggattt ccgcttcgag
    13381 ggctgtaccg cccatggcag cggcgatccg catttgggtt ccaccggaga aaaagattcc
    13441 gctggctttg cgaatcaact tgatctgttc ggctgtgccg gcctcggaac gtttgcgaaa
    13501 ctcaaaagag accacttctt tggcgccgaa agctttgaac agttcaatat attctttgcc
    13561 cgtatcgcgc cgagccgaag cctgcggcag aatgacaatg cgcgcctttt ttccacctgc
    13621 gcggcgcaga aattcctgca gcacggtcgg gtctttgcgt tcatcaagtg cgccgcccat
    13681 gggcatcaat gttgttgtgg tcatgaactc accttgaggg attcgatcaa gttgcgtgcc
    13741 gcagagagga tgtgatcttt catggcgtcg cctgcctttt taccgtcatg cttctccaat
    13801 gcatccatga tggcgcggtg ttccaattgg tcctgcatat cgcgttgcgg cagcggttgc
    13861 tgggctgtta cggcaaaatt gccgatcagc atggtgggaa aggcctccca catctgagtc
    13921 agtgtgcgga tcaaatactc acgcctgctg atcaggaaga tcgtttcatg aaatttacgg
    13981 ttgactttgc ggtattgggc gatctgttcc ggcggcagag attccatttc agccaccatc
    14041 agtttcaaat ccatgatttc cccatcggta atatattcca cggccttttc agccgcgcgt
    14101 ccttcaagaa aggcacgatg ctcgtacaaa tcttcaatat cttccatcga gagtgccacc
    14161 acacgcaccc cgcgataggg tacatgctca atcaagccct ctgccgccag ttccttcatt
    14221 gcctcacgca ccggcatctg gctgaccccc aactcctgag ccacccgttc ctgacgcagc
    14281 cactcgcctg gcttgaagcg cccatcaaaa atggcagccc gaatttggtc tgcgacaagt
    14341 tttctcagct ggcgatggga gggaagttcc tcaagcatgg gcctgtcctt gatattagat
    14401 ataatatcta agatagcata ccaacaggca agagtcaagc attccccggt attttaaggt
    14461 cgccagttct gcaggaaata aaaaacggct cgtttgagcc gtttttcaag aattacgcca
    14521 gatatttttt cgcctcagca ttcgaagcaa ggctttccgc caccatttgg cgagtccaca
    14581 cccccatacg ccttacggta aagcctggaa tccagtcatc cacatataaa acgggtttgc
    14641 acaggttgaa tggaggcaat tcaaggttca gctgacgcgc ccctcccgtc acaatatcgg
    14701 cattggcagt acattccgta tgaatggacg taaacaggta ctgaatatcg ctgcgcacaa
    14761 aattcgccag cgtcttgaaa atatcatcat aagaaaaatg gaacaggcaa tcgcggcaca
    14821 tccagaattc agaagaaggc aggggttcgg tgatgacatc gagtgaaata aagcgggtgg
    14881 aggcatcgcc atatttgagt tggttctgct ttatcaactc ctccacgatg tcgccgccaa
    14941 tgtattccat gccagcaggg cgctgaacag cctgaaacca gttgtaatcg ccgcaggggg
    15001 catccaaaaa ctttttcacg ccaaagcgat tcatcaacag cggaatctcc ctgcggacat
    15061 tttccgtgta ctccattgtt gaaccagccc ccgagacact ttccttgtta ccccaaaagt
    15121 tgttgaaata ataatcagtg aaaacgctgc ggggatcggc aggtttgttt tcaacgggcg
    15181 gctccaccac agcaggcgca ggcgttttca cgggagcagg ctgtggtttc cgtcgttttt
    15241 cgacgatcca tgccacgatc cgttcgccgt tcggcagaag caaaagcata tttttcacaa
    15301 aggtcttgaa agcagccata gggagtcctt gaaatgaatt caaatgcccg cccattatat
    15361 atccctttca aagcaatttt gtgggaaaat cagcggcatg tcaaagcacg atgtcctcat
    15421 catcggcggc ggcccgagcg gactctccac cgccctccat ctaaaaaaga ttgacccgca
    15481 tctcgcctcc cgaatcctga tcctggaaaa ggagcaatat ccgcgcttca agttgtgtgc
    15541 aggcggactt gtcattgatg cggagatcat cctgcaacgc ctcggcctcg atgtaaccga
    15601 agtgccgcat gtaaacgtgg atagcatcca tttcgatttc atgggcagcg gcctaaaggt
    15661 gcatgtaccg aacaggcatg cattacgagt cattcgccgc aatgaattcg atgcctggct
    15721 tgcgaagaaa acccttgaag caggcattga gatccgcgaa ggagtggcgg ttatgaacgt
    15781 gactccgacc gatgacggtg taaccgttga aaccgatcag ggtgtttttt ttgcccaggt
    15841 cgtcatcggc gcggacggct cgaacggtat aacccgcagg tgcattttgc caaatgcgcc
    15901 catttccacc gcccgcgtgc tggaagtttt aactcccgca caccctcaag tatcaagggg
    15961 acgggagaaa gatgcatttt tcgatttctt ccctgtgcca aataatattg caggctatgt
    16021 ttgggatttc cccaccctgg tcaatggtga aaccatgcgc tgttggggag tgtacgatac
    16081 caatctactt gcagatgaga agcggcctgc cttgaaagaa ccgcttgcca gggaaatggc
    16141 acgtcatggc tttgatctaa accaatttga gcttaagggg catcccatcc gctggtatgc
    16201 gccgcagaat cagatctctg tgccgcgggt gttgctggtg ggtgatgccg caggggcaga
    16261 tgcgatcttt ggcgagggca tcagcatggc gctgggttat ggagcgatcg cagcgcagga
    16321 ggtcagcgag tcgtttcggc gcagcgaatt ttcattccgc agatacaggc atcggtttgc
    16381 gcgaagcgga ctcggtcaaa cgctgtttgt acgctgggtc attacgaata tcatctatcc
    16441 catcaagtgg aagtggtttc aaatcctgtt atggagaatt gccaaaccca tcgtcctcat
    16501 cattgcatgg gtctttgtgc tgaattggag caaacgaatt aaatgatccc agggctggac
    16561 ggtttacggg cggtggcatt tctgatcgtc ttcttttttc acacacgcaa tctgcccttt
    16621 ggttggatgg gagtgcagtt attctttgtg ctttcggggt ttctgatcac agatattttg
    16681 ctgcgcatga aagagaagtt gccgcagcgc gaatttttca tcaagtttta cgggcggcgt
    16741 tttttacgca tctttccact ttattatttt tacctgatcc tgcttgcagg gcttgttttt
    16801 gtactaccgc cgttgaacat agggttgctg aatgcggagc ttggcaaatc ctttatcaat
    16861 caaatttggg tggcggcttt ttacctgtat gatttttttc atgccagcgt tttttttgag
    16921 agaagccgtt tctttaccca tctttggtcg ctctcggtgg aagaacagtt ttatttgttg
    16981 tggccgctgc tcattttcct tgtgccgcgc caaaaattca agcagttgtg ccttaccgcc
    17041 atcgggcttg gatttgtctt tcggttggtc atcaccctgg tctatcgtgc ggggatattc
    17101 ccgtttctgt taaatgaccc gcaacaagcc gtgaacgttt tgccgttctc ccaccttgat
    17161 gcgtttgctt ttggtgcgta catctcacgc tttgaaattc cgcgtccgcg cttgcagctg
    17221 ggtctgcttg cgatttttgt ccctgccctc ggtctgctga cagattttct ctcaaaaggc
    17281 tccgttgttg tcgcgtcact gggttacgac ctgccgatga ccggctttta caaggaagta
    17341 tggggctaca ccctgctgaa ttatcttttc gccgtgttga tccattgtgt ggcacgcact
    17401 gaactgctca attccatact ggagagcgcc cctttgcgct atcttggaaa gatttcctat
    17461 ggattgtatg tctatcacta tgggatcatt gcactggcgg ttgcagtctt ccgggaatac
    17521 ggcctgccct attctgtgcg gtcaccgcaa atgttcgtcg ccgcgatggg gtcaacccta
    17581 ttgatcgcca cgttgagttt ttatctgctt gaaaaaccga tcatagatct gaaggataaa
    17641 ttcttccgaa tctaaataac atttatcatc ttcctttttg gaattttcaa cgtataatac
    17701 tttcagggaa aacacattgc catgggcact gcagacctgc atattcacag catctattca
    17761 cacgacgcca ccactacagt acgtgccatt ctcaaacaag ccgcagatgt cggcctggat
    17821 gtgatcgccg ttaccgatca cgatgaaatc cgcggttcgc ttgaagcctg cgaactcgca
    17881 cccaagtacg gggttgaagt catccccggc gcagaagtca ccaccaaaga tggtcacctt
    17941 gtggcacttt ttgtggaaac cctgccgcct gcggggcttc cacttgtcga tacgctgatc
    18001 cacatcggga aacagggcgg catcgcgatc gctccacacc catttaataa cctgccaggc
    18061 agtctctcaa tggaatccgt tgtcagtgcg ctcaccaatc cgcgcgcaaa gagcgtactc
    18121 aaaggcatgg aaacgcataa catgggtacg caatcattcg attcagtggc gcaaaaactt
    18181 tccatttacc tgccgctggc aaagattgca tccagcgatg ctcatattta ttgggcggtt
    18241 ggagcaggcc gcaccgaatt ccccggcaaa accgcgcagg accttcgcac tgcactcgaa
    18301 aagaacacca cagttcccat cccttatcat gaggaattct ccgcaaaggc catcctcagc
    18361 tgggtgcgcc gcgtaacttt gcgcaggttt ggttacgcat ctgacagtat ctctgcaacg
    18421 cagcccatca acacacaaaa gctgtccgaa tcctttattc aaaaaacaaa aaagaaaata
    18481 ataaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncccg cacaaaaacg
    18601 attttcttca tgatttgacc cgtttgataa aacccggcgt attcaattcg cgctcaagca
    18661 aataggtgaa atagccttgc atcaaggctt ccgcttcctt ctggacctcg aaactgggat
    18721 gtgcgcgaga agcatctctg taacttgacc tttggaagtg ccgaaaatat ttaagcgctt
    18781 ccatggagat cttcgccaga ttgggcaacc ccaacccgca ctgcggacaa atcacccccc
    18841 ccgtcacaaa cgaaaagtat tgatcttcag gcaagatctc ccgtccacaa ttcgcacact
    18901 cgaacaactg tggacgaaag ccgacaatat ccagcagcct catctcataa taacggaccg
    18961 gcagccaggc ttcgtcctct ttttcgatcc tgcccaaagt atccaccaac agcttgaaga
    19021 gcgatggatt cccgccttcc tcttcataag aaaaccgata tagcaattca acgatatagg
    19081 ccgcataacc tgtcttcacc aggttatcgt gcaaaggcaa aaaggcatcc acggtttcag
    19141 cctgggtcac aatcaacagg tcacgcccct tcgccaattg cacagtgaca tgggtgaacg
    19201 gctgcaagtg ccccgccttg cgtgaattga tctttcgcgc acctttggca agtgccttta
    19261 ccattccctg ttcacgtgtg tacagcgtca gcaaacggtc cgcctcaccc caatcggtgt
    19321 ggcgcacaac aacagcaaaa gcacgaaacg aacggaattc ggtcatggga gataattcac
    19381 agcaacgagc gcggctcgtt accatgcaac accttatact ttactgcaaa tgttttggcg
    19441 taaatgcctc aggcagcaga tcggccaccg ttaattcttt atgcaagttt ccatccccat
    19501 ctgcgatcag aacaactgta tccaggccga attcagccat cacctgcctg catccgccgc
    19561 agggggaacc gccattattg gtcaccactg tgatcacatc aaattcagac tcaccctcag
    19621 aaaccgcctt gaaaattgcc acccgctcgg cacacatggt ctgcggatat gccgcatttt
    19681 caatattcac gccggtgaac acctttcccg attttgttcg cactgctgcc ccaacgggat
    19741 aatttgaata cgggacgtaa gctcgtttgc gaatttcaac agcttgtttt atcagtgttt
    19801 gtcgttcatc attggttaga tccatagttc acctgtccat tttctttctg gcctacttct
    19861 tcgttttacg ctccaacttc ttgatcgccc gggcgggcat ccctaccacc agggtatctt
    19921 ccgaaacatt ctttgtcacg acagcccctg cgcctgtccg cgcacctgcg ccgatcttca
    19981 ggggagccac caacatggta tcagacccga tgaatacatc ctcaccgatc tcggtaggat
    20041 gtttcttttc gccatcatag ttacaggtga tggtccctgc accgatattg gtattcgaac
    20101 cgatctgggc attgccgatg tatgaaaaat gtcccatctt cacaccatct gcaagatacg
    20161 aatccttcac ttcaccaaag ttccccatat gaacatgatt ccccaaatgc gctcccttgc
    20221 gcaaacgggc gaacggtccc atatccacat ggtcatcaag aagggcgcct tccatcaccg
    20281 aagccagtac cttgcaatga ttcccgatct ttgaatcatg gataatggtg ttcggtccga
    20341 tcacatttcc ttcgccgatc tctgtcctgc catgcaaata tgtattcggc atgatgaccg
    20401 tatccctgcc gatgcttacc ccggcttcaa tatatgtgga agcaggatcg attattgtga
    20461 caccgttcaa catatgctgt tggttaattc ggcggcgcat ggcagcttcc gcatccgcaa
    20521 gatggaggcg ggtattaatt ccgatggtct cttcgacatc atccatcacc gtggcctgca
    20581 ctggcaaccc agcttgggaa gccaattcca ctgtgtcggt cagataatat tcacccttct
    20641 tgggattctt tggaatgcgg tgcaaggcat cccaaagcca attggcatcg aaacaataac
    20701 cgcccacgtt caattccttg acctgtaact gctcaggcgt cgcgacatat tcctccacaa
    20761 tggcatccac ggtaccatcc gccttgcgga tgatacgccc aaagccacgc gggtcatccg
    20821 catgaacggt gagcatggag atcggtcctt tgttcttctt ctgcgtttca accaatgcct
    20881 gcaaggtctc tccacggagc aggggcatat cgctatagca aacgactact agatcggtct
    20941 tgcctcttaa taggtcctcg gcctgcatca ctgcatggcc ggttcccagt tgcggttcct
    21001 gtaaaacggt ttgtgcagaa tcgcccagat atttcgtcac ttcttctgcg ccatgcccca
    21061 ccactacaat tggcttctct gtggtggact gactgatcgc ctgcatggca tgccagatga
    21121 gcggcctgcc tgcaatggga tgtaaaacct tgggcaagct tgatttcatg cgggttccct
    21181 gcccggcggc aaggagaact gcggtcactt tcatgatgtc acctcgataa ggatatgcgt
    21241 cttgctactt cactcgaaac gaacagggaa taacgaaaat aggatttgcg cggcaatgca
    21301 ttgctcgacg acaaatccta tttgagaaaa gaatccccgc gtaagcggga tgtatggctg
    21361 gggcacctgg attcgaacca agatccacag ctccaaaggc tggtgtgctg ccattgcacc
    21421 atgccccagt gcgggcaaaa ttgtatcaca aattttttac tttccatctc caggaagcag
    21481 ccccagtttg cgggcatctt caagtgagat cacttccttg ctggcaggtt tgttgccgtg
    21541 tttttctgca gcctgattcc agaaatcatc catgttggcg gtttggtctt ttgtggcagc
    21601 ggcattcttc aatagggctt caagctcggg ggaaggagct gcttcagcgg gtttctccgt
    21661 cggtttaata gaagcaggta tcgggatggt gggagccgct gccgtcgcca gaggaacggt
    21721 cttcggggaa attgactgca actcgccggt gaccccaagc gagcgcagag atcgttccac
    21781 ttccttgcgt aaagcaagca gggcgttcac agattcaagg atgcgatctt cacggacaag
    21841 tccattccct gcaaccagca aggcatacat gggtgcgaca gggatgaaca ggaggtcatt
    21901 atcgccgctg ctgaacacat gataggaatc gagtttttcc tgatggttat actttgccac
    21961 tttaaggctc gcgttgtgaa tcgccatcaa agtggagaag agcgaaactt ccatgctttc
    22021 gtcgcgcagt ttgccggtgc gggcctgtac aagcccgcgt tcattcaaaa ggaagactgc
    22081 ttcggcgtta atatcctgtc tgaaatttgc cagcaggtca gatacacggg catgacgtcg
    22141 ttctgttttt tcgtcggagg ggcgttctgg ggggaaaatg gtttgaacga atccaagtcc
    22201 gcgttcaact acatccaaaa aatcggcgag gggaatcggt ttatcgaaca ccgcaagtgc
    22261 accggcgttg agcatttctt cacgtacttt gcggtcggtc agaccagtga tcaaaataac
    22321 ttttgcttcc ggatggcgca cacgtacctt gtgcatcaat tccaaaccac tcataccggg
    22381 cagtttgtaa tctgtgacaa gcagatccac cttgtggcgg gacgactcga tcaaggcttc
    22441 ttcgcctgaa ggggcttcga atatcttgat ctccgttttt ttgagcgttt caagtgtgga
    22501 acgcaaaaga cgcaggatat cgcgctggtc atccacaagc aaaatattgc gatccatatt
    22561 ttcctacttc accatccaac aggcgacaaa atgaccggca gcagcttcac ggaattcggg
    22621 ctcctgctcg gagcagattt tgtcggcaat ggggcagcgc gggtggaaac ggcagccctt
    22681 gggtgggttc aacggactgg gcacatcacc cttcaacact tcacgctggc gtttgagctt
    22741 cgggtcgggg atcggaatgg cagacatcaa cgccttggta taaggatgca acggattctt
    22801 gaagaggtcg tcgcgggagg tcaattccac cattttgcca aggtacatca cagccacacg
    22861 gtctgaaata tgctcaacca cgctcaggtt gtgggcgatg aacaggtagg tgagaccaaa
    22921 ttccttttga aggtctttca aaatattcaa cacttgcgat tggatggata catccaacgc
    22981 agagacaggt tcatcacaaa taatgaaatt tgggcgtaac gcgagcgcac gcgcaatacc
    23041 aatgcgctga cgctgaccac cggaaaactc atgcggatag cggcgggcgt gataatcttc
    23101 aagaccgacc tttttgaggg tttcgatcat caggtcgtag cgttcctgcg gcgaaccgat
    23161 cttgtgaata tgcaaacctt ccatcaccga ttcgccaatg ggcacacgcg gatccaaaga
    23221 agcgtacggg tcctggaaaa tgatctgcat atggcggcgg acatccttca attcactgcc
    23281 gcgcaacttc aatacatccc gcccgtcgta ttgcacagac ccggaggtcg gctccaccag
    23341 cctgagcatg gaacggccaa tggtggtctt tccacagccc gattctccca ccatgccaag
    23401 ggtttcccct tttttcacag tgaatgaaac atcgtccaca gcctttaccc aattgaccac
    23461 gcgctgcatc aacccggcgc gtaccgggaa gtacttcacc aaatgttcaa cttgtaccag
    23521 gtcttctgtt ttctgcattt ccgttgtttg actcatgctc atcgactcca gacgattgtt
    23581 tacgataacg cggcaacctc cccaaacggc cgcctaaaaa actaaccctg cttggccttc
    23641 aacggcgctt ggtgatcttt tgcgttttga tacagccagc aacgaacctt gtggccgggg
    23701 gcggcatcag tcagctcggg ttttacttcg gtgcaaatct ttaagccgaa tttttcacga
    23761 gccgtacaac gcggcgcaaa gcggcagccg ggcggcaggt tcaccaaatt gggcacggaa
    23821 cctggaatca catcgaggcg ttccttgatc tggccaagga ccggaatgga accgatcaag
    23881 ccctgagtgt acggatgcaa tggttgctcg aaaagcgaat tcacttcggc ctgctccacc
    23941 acttcacctg catacatcac cgccacacgt tcggccatct ctgccaccac accaagatca
    24001 tgtgtgatca aaatgaccga ggtccccatc tttgtgcgca aatcgcgcat caggtcaagg
    24061 atctgggcct gaatggtcac atcaagcgca gttgtgggtt catccgcgat caacagttcc
    24121 ggcgtacaag ccagcgccat ggcgatcatc acacgttgag ccatgccgcc ggaaagctcg
    24181 tgcggatacg catcggcgcg gcgctcggca tctgggatcc ccaccatctt caacaattcg
    24241 atggcacgct gtcggcctgc ttccttgcca actgctttgt ggatatttaa tacttcggca
    24301 atctggttat ccaccttgaa caccgggttc aaagcggtct gcggctgttg gaagatcatt
    24361 gagatcttgt tgccgcgcat ttttgtcatt tcgtcttcag aaaggttgag caggtttttg
    24421 ccatccagca aaatttcccc ttcagtgatc tttccgggcg gtgcgatcag gcgcatgatg
    24481 gaaagcgagg tcacgctttt tccacaaccc gattctccaa cgatacccaa tacctcaccg
    24541 ggatacactt caaaactgac gccatcaacg gcgcttacca ccccgtcttc tgtataaaaa
    24601 taggtcttaa gatttcggac ttccaacaaa ggcttccggt cagtttttgt cacagcctgc
    24661 tcctgagaat gaattacggc gattatactg cctctcgctt ctttctcaaa caattataac
    24721 ttccaacaac aaaaatgcga attgcgccac ct
//
LOCUS       DAOQJC010000056        24395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_903_length_24395_cov_9.583032, whole genome shotgun sequence.
ACCESSION   DAOQJC010000056 DAOQJC010000000
VERSION     DAOQJC010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 24395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24395
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_903_length_24395_cov_9.583032"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..548)
                     /locus_tag="PLE39_10160"
     CDS             complement(177..548)
                     /locus_tag="PLE39_10160"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNE68734.1"
                     /translation="MQSTAKNVTEYLKESPSERKAALTQLRRLCRASLKGFQESMMYG
                     MPVYTRNGEAEVGFASQKNFIALYILKTDVMDAHRDLLKVKGISLGKGCIRYSKPEKI
                     DFKVVEMMLKATQESTGEVCD"
     gene            complement(695..1054)
                     /locus_tag="PLE39_10165"
     CDS             complement(695..1054)
                     /locus_tag="PLE39_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68735.1"
                     /translation="MGVGYILVNYSKKELISFNHIGANKARELAGNPVSAAMVTWYML
                     QHTGDTVAFLSDTYGEWCFPEGTRDEISDYRDATDEVVKSLIEVEILVDLGKTWVDSD
                     EPDKIYIRALENVWMKF"
     gene            1405..2292
                     /locus_tag="PLE39_10170"
     CDS             1405..2292
                     /locus_tag="PLE39_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE68736.1"
                     /translation="MRLKADLTLLVVSIIWGSAFVAQRVAGQQGSVYLFNGARYVLAA
                     LVVLPFVKRVNLTSMPHDQYKWMFIAGFFLFAGSALQQAGMVYTTAGNAGFITALYVV
                     LVPFALFFFWREKPHWMSVVAVVLAGVGAFLLSTGGRFEVQKGDALEVVGALFWTFHV
                     IIIGKFAAKFEAMSFSVGQLLVCGVLNLGVGLVSESMMTFNWQLAFAIGYTAFFSLGL
                     CYTLQVWAQQHTPPADAALILSLESVFAVLSGWLLLDERLAVIQIFGAVLIFIAVVLS
                     QFKEWTSGKIDHDHLVEGR"
     gene            2297..2887
                     /locus_tag="PLE39_10175"
     CDS             2297..2887
                     /locus_tag="PLE39_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE68737.1"
                     /translation="MTEERNLIEELDLSFHPLTQKLWHDFEMLFGRRGACGGCWCMHW
                     KLRGKEFTESAGDTARQMQKSIVDSKTVPGLLAYSEGYPIGWIAVEPRSAYPKLAHSR
                     TLKPVDDQEVWSITCFFIEKKHRHKGITVELLKAAVDYVKGRGGKIVEGYPVDAKQEA
                     PATFVFTGTASAFHHAGFVEVARNSPTRPIFRFVIE"
     gene            2950..4209
                     /locus_tag="PLE39_10180"
     CDS             2950..4209
                     /locus_tag="PLE39_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68738.1"
                     /translation="MSVTFQPPSKSFTARDYQFIALVSIVVVAISIGLVMLNLTLKDG
                     GDFYVHWFAVRVFQNDRLDPYSGEVPARVQQQVYGGPAAQGDEPYILDTPFHILLLYF
                     PLTLLSDPLLARAIFTLILQLALFALAFISLRLTEWEVPIIFYVLFPLLAIFNFYTFQ
                     AIYEASPVLLLGLTYAEILIALRADLDEVAGALAAVSLYYWEVGAPFLVLVALRVYYE
                     KRTRVFAGFAMASLILLVLSFMAYPNWFIPFLRATVNNIKADFGFNVHSIFIRIWPAF
                     GGTFAWLFIGLLVVALGYEWSAARSGDFRRFYWAACLSIAAAPLLGFRTEMENLAVLI
                     IPLALIFAIVHDRWYKIGNFLTILLMLLVGTLPWAVSLFLVPRFGIKAEEGLFLFLPL
                     FTFIGLYWIRWWAIRPPRILSDIASRL"
     gene            4225..5784
                     /locus_tag="PLE39_10185"
     CDS             4225..5784
                     /locus_tag="PLE39_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68739.1"
                     /translation="MNSRTYFFLFLFGLAVPVFIAQFQPIPGYLDSDYYYAGGIQLAT
                     GKGFTEPYLWNFLDGTTSLPHPSHSYWMPLASIVSALGMWLTGQTTFASARLFFFLIA
                     ALVPPLTAALAYAFSQKNELAIASGMLAIFSVYNAPFVGVTDNFGLFMLFGGLYFIIA
                     TQLMQNPDRMRNWLFLGLLSGLMAFSRSDGLLWLPLTFLFALWRARKISSFIPHPSSL
                     LLILLGFLAIMSPWYLRNLNLYGTPMAPGGSRALWLENYDQTFIYPPTLLTKESFLAQ
                     GWEKILDDRLWALNMNLQSGFAAHGGIILFPFIVAGIIYYRKDDRVKLAALAWLILFL
                     VMTFLFPFAGARGAFFHAGAALQPIWWTLAPLGLESILVSLRKRGWGNDQNRFVFRSA
                     LVMIAMILTVYVVYLRVFTLGWGEIDADYKAVDQFLVEHGVGEEDIVMARNAPGYYLL
                     TGRSAISIPYGGENALLEAAKQFDAYYLVLEPEAALDPIKDLFQNPNSRNRFVYLGDV
                     DGARIYKLETK"
     gene            5750..7243
                     /locus_tag="PLE39_10190"
     CDS             5750..7243
                     /locus_tag="PLE39_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68740.1"
                     /translation="MVRVFTNSKLNKISNRDLLMLAAAVVVVTSIYLLASYFNYGIGF
                     PLDDSWIHQTYARNLAQRGEWAFRPGVPSAGSTAPLWSALLALGFLFSLSPYIWTYLL
                     GAFTLFALTVVCEWAVRTLIDSYRPHLPWVGIFFAFEWHFAWAAMSGMETLLHGLIVT
                     TVLVLLMTNTRRYLTLGLLTGLSAWVRPDGLTLLGPVLIMVLIHEQDTRPRLIALLRC
                     LIGFGSLFVPYLLFNLAIGGTPMPNTFYAKQAEYASWQTISIFSRLGQMFLQLLVGPS
                     LVLIPGVMGWLVKSIKQKMWGSLAALVWCAGYFGLYISRLPVYQHGRYIMPAMPIFFL
                     FGLLAFADFDKSRLFERYHWIGQTIWRASLVMLSVAFIFLGAQSYSQDVALIESEMVV
                     TAKWVAANLPPDALIAAHDIGALGFFDTHALIDLAGLISPEVIPFIRDEPKLANYLNQ
                     QGADYLIAFSGFYPAMTGSLEKVFVTNSVVTLKYDEENMAVYLWKTP"
     gene            7300..8001
                     /locus_tag="PLE39_10195"
     CDS             7300..8001
                     /locus_tag="PLE39_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE68741.1"
                     /translation="MNQITLLIVDDHPLFRQGVVDALSLEPDMRIIAQSAAGDEALEL
                     IRTKKPTIAVMDVNLPGMNGQQITHQASQDRLPTRIILMTGYDDVEQAIHAALAGAHG
                     YCSKDIEPQSLSRIIREVAEGKYVFENNVFTRRELELWVEDRIEGARRSYSEPGTPFH
                     PLSDREMEVLACVVRGMSNKEIATLLGISHQTVKNHVTAILRKFGVEDRTQAVVYALK
                     RGWVTLKDTNIHPQE"
     gene            8005..9018
                     /locus_tag="PLE39_10200"
     CDS             8005..9018
                     /locus_tag="PLE39_10200"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNE68742.1"
                     /translation="MTSTEPMDFQSELDETQRALREVTLMIEQSQGELGKITQRNTAI
                     TSHLQQLQKQGTTVDDLKMSYDSALDVQQRLFVMRGQLEKLQNDKSHLEKYKTVLENI
                     VSGAASGGDAAGATPAAGGKSQMAGIEMIVNAQEAERQRLSRQMHDGPAQALSNFILQ
                     TEIAMRLLDVDPAQAKDELGNLKTSAMGTFQRVRSFIFELRPMMLDDLGLVPTLRKYA
                     DAFKEQASLDVSVTVTGTERRLEPYLEVMIFRAVQELLGNASRHSQATLIKVQIDLGN
                     DTIRVSVEDNGKGFSPEILKESSNLGLKLIRERAEMLGGSFEVDSAAGSGTRIAFSVP
                     AKV"
     gene            9292..10374
                     /locus_tag="PLE39_10205"
     CDS             9292..10374
                     /locus_tag="PLE39_10205"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HNE68743.1"
                     /translation="MRRGRTLIFVVLILIIGLAVAVAAYFQFLRPAATPTQPVTTVDV
                     YMAGQPIAQGSEITPELLTTIKIAPENLVSVMYTVEMQSQLIGKVAKYPLDQGVIITQ
                     AMVSDATSAVPISGPQWAALIPTGMTAISIPADRLSLASYGVNNGAHVNVNACFLFVD
                     IDPSFQSALPNQTGQLTGTGFTPEGLPVLSLGVSASGSSQGRLELDPSIQQPYYLMPS
                     ERQRPRMVCQMLLQDVVVMKVGDFPSDPAASLSAPAPVATDPETQQAAPVPPDVVTLI
                     VLPQDSVTLSYLIHTDAELTLTLRNPTDQLRQATEASTLQFLLSQYNIPVPAKLPYSL
                     EPYTVSFCNLSLGTSAICSQTAAPPQ"
     gene            10507..11748
                     /locus_tag="PLE39_10210"
     CDS             10507..11748
                     /locus_tag="PLE39_10210"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE68744.1"
                     /translation="MAADKIRILIVDDIAETRENIRKLLQFESDVDVVGVARSGKEGI
                     QLAQELDPDVVLMDINMPDIDGITATEQVRKKSPHVQVVILSVQNDQSYMRRAMLAGA
                     RDFLNKPPLGDELISAIRRAGEMAHTERAKSAQQSFVGAAVAGSGGAAMNFAPVHKGK
                     IITLYSPKGGTGCTTLAVNLAIALNNEDTRTSLVDANLQFGDVAIFVNEPGKNAIIEL
                     APRVDELEPEVVDDILINHGASGVRILTAPQRPEYAEKISAEQFTKVIQYLQRMYAYV
                     VVDTSHILSDVVLSIFDVSDVIVLVTTQEIPAIKNARLFLDLLQTMGINKNRVIFTMN
                     RYDKRISITPERVSENLKHEVSVTIPLDEKIAITAVNRGVPFMLDNKSQPIGRGIFSL
                     AEAVRSRLTTLESENDMSDRR"
     gene            11760..13241
                     /locus_tag="PLE39_10215"
     CDS             11760..13241
                     /locus_tag="PLE39_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNE68745.1"
                     /translation="MSLLRRIEQGQQGGGQQQGNGNTSGTPSPTPPAKQPGSGQGGEG
                     SGGGGDTSRLSSLQARRVSAPITSPQAGSYFDLKTRVQNKLLAEIDPSMDVSRTDDVK
                     RTIQGLFEQILTEENIVLSRPERARLFEQIAAEILGLGPLQALLEDDTITEIMVNGPK
                     NIYIERKGKLHRVPVTFESNEHVMRIIDRIVAPLGRRIDESSPYVDARLQDGSRVNAV
                     IPPISLIGPVLTIRKFSKNPISIEQMVQFGSISHEAVQFLKACVEARLNIVISGGTGS
                     GKTTLLNVLSSFIPGDERILTIENAAELQLRQEHVVTLESRPPNIEGRGEITIRDLVI
                     NALRMRPERIIVGECRGGETLDMLQAMNTGHDGSMTTAHANSPRDAIARIETMCLMAG
                     MDLPVRAIREQIAGAVDVICQQARMRDGSRKVIAITEVSGMEGDVITMTDIFTFEQTG
                     MENGRIIGRTRPTGLRPKFMDKIEAAGIHLPPSIFGIGERRRY"
     gene            13267..14220
                     /locus_tag="PLE39_10220"
     CDS             13267..14220
                     /locus_tag="PLE39_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNE68746.1"
                     /translation="MLNIIIIGGVVLIILLIIGVVVSSNSERAVVEQRLAQYLDDDKQ
                     DIDSAAQNSVLTEWVSKRVEKTSFGDRIARDLARADLKFKAGEYFILIIVSILLGGLV
                     IWFLGNQHWASAVIGAVAGAFAPGIYVRRQQKQRLQKFNDQLSDMLNLMVNGLRAGYS
                     TMQAMEAVSKELPSPINDEFRRVVQEMQIGIPMETALDNLLRRIPSDDLDFVVTAINV
                     QREVGGNLSEILDTISFTIRERVRIKGEVRVLTAQVRTSGSVLSLIPFFLVLILWFLN
                     PEYILAVTAGGPIITAAIICVVLGLIFTGYFIMMKIADIEV"
     gene            14223..15182
                     /locus_tag="PLE39_10225"
     CDS             14223..15182
                     /locus_tag="PLE39_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNE68747.1"
                     /translation="MGTIVGIILGIVVVGGVIALIVVGMRARQGGGDESDPILSRLAE
                     ATQRGETVSLEDIELQQPFIQRVIVPMIEKVGEVSSRFTPQKLLQETTLKLELAGNPG
                     RIDAATFLATRFFGAGIFGGGLLLIALASQTWPVGRIILVVGFFTVIGFFFPQLWLQS
                     RINSRQAEVRKALPDALDLLTICVEAGLGFDAAMSKVAEKWENELSLMFGRCIREVQL
                     GKTQREALRDMADRLGLPELTSFVAAVIQSQILGVSLAKVLRIQSDQLRVKRRQFAEE
                     LAHKAPVKMIIPMALLTFPSIMIILMAPAAFQIAGAFGPFLGQ"
     gene            15509..15982
                     /locus_tag="PLE39_10230"
     CDS             15509..15982
                     /locus_tag="PLE39_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNE68748.1"
                     /translation="MRNALHKLKYRRDLALGDALAAHMSDFVRTLNWQADMIVPIPLG
                     KQRYRERGYNQVAMIAKPLALSLRMEFAPQTLSRRKETRSQVGLTKEERRVNVHEAFQ
                     AGTGVKGKNVIVMDDVSTTGSTLSSSAEALFRSGARNVFALTVARALPHHGLRHA"
     gene            16025..16588
                     /gene="raiA"
                     /locus_tag="PLE39_10235"
     CDS             16025..16588
                     /gene="raiA"
                     /locus_tag="PLE39_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNE68749.1"
                     /translation="MSNKVEVQTRNIRLTEKIEEYVNKKAGNLDRYLPTIDSARVELA
                     HHQAARNATDRNVAQITVYGKGFTLRTEERADEALAAFDRAIDNMQTQIERYKGKHQH
                     GRGDGRSAAEAAEPIIDDETGELSPLVARKKKFVLYPMNEEEAVVQMRNLGHDNFFIF
                     YNAETSKINVLYRRRNGSYGLIEPELG"
     gene            16681..17304
                     /gene="folE"
                     /locus_tag="PLE39_10240"
     CDS             16681..17304
                     /gene="folE"
                     /locus_tag="PLE39_10240"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNE68750.1"
                     /translation="MEFDDVNESDVEGSAIDNAAIETAVTQILNAVGEDPQREGLQFT
                     PRRVARMYHELLGGYKMDPVAIINGALFNVKYDEMVLVRDIEFYSLCEHHMLPFMGRV
                     HVAYIPDGKVLGLSKIPRIVEMYSRRLQVQERMTRQIADFLRDLLKPQGVAVVVEAMH
                     LCSMMRGVKKHDARMTTSAMHGAFRANLATRQEFLANISRGSAPLQL"
     gene            complement(17413..18171)
                     /locus_tag="PLE39_10245"
     CDS             complement(17413..18171)
                     /locus_tag="PLE39_10245"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNE68751.1"
                     /translation="MIELFRNLFSPPRHMILLVAAAWFGLSFAEKRAERHGIQKDDLN
                     NVIFYGLLAFVIGGRVSFILQNLPTFLKSPSGIVSINPDIFDAFGGLAAAVIAATIFG
                     QRRGLLLWPTLDALTPFFATLAVGMGLSHLAAGTAFGKATDVPWSIELWSANRHPAQI
                     YETLASLLIFGLIWRQKHDQRPGIHFLTFAALISASQLFLQAFRGDSTLIFNGVRQEQ
                     VVAWLALAVCFMLLEARFKSSPKAETDSAAVHAE"
     gene            complement(18173..18715)
                     /locus_tag="PLE39_10250"
     CDS             complement(18173..18715)
                     /locus_tag="PLE39_10250"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNE68752.1"
                     /translation="MQKTQRIILYVLLLIAGAAWTSLSTDKAGTSTSGNIPAPQAGFS
                     APDFSLKPPTGESYTLSELRGQAVLVNLWATWCPPCRAEMPTIEAMYQEYKDQGFIVL
                     AVDMTYQDDPFEVVPFTQEHNLTFPILLEETGDVAAAYQLRSLPSSYFINREGIISEV
                     VIGGPMSEALLRTRIEQILK"
     gene            18796..19557
                     /locus_tag="PLE39_10255"
     CDS             18796..19557
                     /locus_tag="PLE39_10255"
                     /inference="COORDINATES: protein motif:HMM:NF016163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HNE68753.1"
                     /translation="MLFTDSVFVGVDPTSGRKSFTYAALDKDLRLVALADGELEDVTT
                     FLAGQKSATVAVNAPAGVNRGLVRARMKKEMLTPHQVRGADIRLAEYQLREHGIVVTG
                     TAASAALCPAWMQSGFELYRKLGKAGFKKFPEKEAQFQILETHPHACYCVLAGQIPLA
                     KPSLEGRLQRQLILYENGVYVKDPMDFFEEITRYKLIKGIWPMELLYLPEQLDALVAA
                     FTAWLAVNKPERISTIGDEKEGLIVLPEKELKEKY"
     gene            complement(19635..20870)
                     /locus_tag="PLE39_10260"
     CDS             complement(19635..20870)
                     /locus_tag="PLE39_10260"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE68754.1"
                     /translation="MKQENSLPQEQLWNIYNCHIHTFTQKNTPGQFYKLLLTDSHSGK
                     MSPLKVVFVLLLPFLLIAFSPLILFMVILDYIRGGAERNSADQNGRMQKVKKKAINRL
                     AALLAAINPASDDLLERYARFLNTSASDSQETIFKQIKHQYPENAKFVILPMDMEFMN
                     LGAMKQSIEDQHADLLKLAAASEGSIIPFYAVDPRHEDIVERVKANLAKDKFRGIKIY
                     PNLGYKPNHERLMDVYKICGDTYPVISHCSPSGIWQYGLSESDRINFGHPENYIPVLK
                     ENKKLRLCLAHFGGAEEWVKHLKGQTPSTGDGRAWVRVISDMLTSGNYPNLYTDISYT
                     IFMPKVNGLYIDLVDYLKVLLSNKQIREHVLFGSDYYMVEQERISEKEASILLRSRLG
                     EDLYRQIAYTNPRKFLGIK"
     gene            complement(20920..21735)
                     /locus_tag="PLE39_10265"
     CDS             complement(20920..21735)
                     /locus_tag="PLE39_10265"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HNE68755.1"
                     /translation="MVDPNFFNEVPIFELLDDEERKVLADQVSVREFKEGQTVFKAGD
                     PGGLAYLVQKGLIHVTIKDANFEDVTVDVADSGGLVGMSSLLAGENHQTTAVATEDTT
                     AIEIDRNDIMTLLHVKPMAGLDMLTIVEKQLRVAHELMRTRVSKNPNEEIEDHETFGE
                     RMADAVARFGGSWGFVITFAVVLIVYTGINSVLKQPWDPYPFILLNLFLSMLAAIQAP
                     VIMMSQNRQDAKDRLRSELDYRVNLKAELEIEELLHRVGKIEEILVEHKNEDE"
     gene            21819..23783
                     /gene="tkt"
                     /locus_tag="PLE39_10270"
     CDS             21819..23783
                     /gene="tkt"
                     /locus_tag="PLE39_10270"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNE68756.1"
                     /translation="MTADLQARAINTLRFLSADGVQKANSGHPGLPMGTAAMAYTIWT
                     RHLRHNPRNPKWAGRDRFILSGGHGSMLLYSLLHLTGYDLPLSELQSFRQWGSLTPGH
                     PEYGLTPGVEMTTGPLGQGFATGVGMAIAASHLAANFNQRGHDVIDPFIYAIVTDGDL
                     MEGVASEAASLAGHLSLGRLIYLYDDNHISIDGSTDLAFTEDRAVRFRSYGWQVLRVE
                     DGNDVEAIDKAIQEAKAESRPTLIMCRTTIGFGAPNRQGTSKAHGEPLGDDELNAAKD
                     NLGWPKEPRFFIPDDVLAFYRKAVDKGRELEHDWKMRFEAYARLHPALGAELQRRLNG
                     ELPSDWKSALPSFPADAKGMATRAASGKTINAIAAKLPELIGGSADLAPSNNTKIDGS
                     PAFQKDSYQGRNFHFGVREHAMGAALNGMAVFGGVIPYGGTFLVFADYVRPSVRLAAL
                     SHIPSIFIFTHDSVGLGEDGPTHQPIEHLTSLRLIPNLVVIRPADANETVAAWKVAIE
                     RRNGPTVLALTRQNVPTLETSAQVEKGAYVLKDFGNPEMILMASGSEVGLVLEAAQKL
                     ADEGKGVRVVSFPSWELFEKQDEAYRESVLPNAIQKRLAVEAGSGLGWERYAKSVISI
                     DRYGASAPAKVIFEKLGFTVENVIAKAKTL"
     gene            23819..24271
                     /gene="rpiB"
                     /locus_tag="PLE39_10275"
     CDS             23819..24271
                     /gene="rpiB"
                     /locus_tag="PLE39_10275"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNE68757.1"
                     /translation="MKVAVGCDHGGFPLKEVVIEAVKAAGHEVIDVGTYSVDAVDFPD
                     FTKKVGEKIQSGEAERGVVICGSGIGAAIAANKMKGIYASICHDTYSAAQGVQHDAMN
                     VLCLGGRVIGPELVKVIVPAFLGAKYLGDEKGGERFAKRVNKIKELEG"
BASE COUNT         5615 a         5848 c         6288 g         6644 t
ORIGIN      
        1 agctgtttct tctccattac attgactcca tgagttggga ttttggaatt acagggttag
       61 ggagcaattt aaaaaccctt tgaaccacta agactcaaag acacggagtt gtaattggtt
      121 ttctttgtgt ctccgtgtct ttgtggttaa gattttagct tttcgcagga ggtttgttaa
      181 tcacagacct cccccgttga ctcttgcgta gccttcagca tcatctctac aaccttgaag
      241 tcaatcttct caggcttgga gtagcggatg cagcccttgc cgaggctaat ccctttaact
      301 ttaagcagat ccctatgtgc gtccatcaca tctgtcttga gaatatataa ggcaatgaaa
      361 tttttctggc tggcaaaacc aacttcggct tcaccgttgc gtgtatagac tggcatgccg
      421 tacatcatgc tttcctgaaa ccctttaagc gaagcacggc atagtctgcg caattgggtg
      481 agcgcagctt ttctttcaga aggcgattct ttgagatact ctgtcacatt ttttgcagta
      541 ctttgcatga tgacctcaca gtgtatttat aaagtgggtg gtgggtggga tttggtatgt
      601 ttgtaagatt tgccccggcc attttgcggg cgtgaatttg tgcctctttg atggtcaagt
      661 tggcaaaatg gatcattgtt tcaggtgcat tttttcaaaa cttcatccaa acattttcca
      721 aggctcggat gtaaatttta tctggttcat cgctgtcgac ccatgttttg cctaaatcta
      781 ccaggatttc cacttcaatc aagcttttga caacttcatc tgttgcatca cgataatctg
      841 aaatttcatc gcgagttcct tctggaaaac accattcacc atatgtgtcg gataaaaatg
      901 ctacagtatc acctgtgtgt tgaagcatgt accatgtaac catcgcagca ctaacaggat
      961 tcccagctaa ctctcttgct ttgtttgctc caatatggtt aaaactgatt aattcttttt
     1021 ttgaatagtt aacaagaatg tatccgacac ccatgcaagt tatcctgtaa tctgatttgt
     1081 gtttctctct tattttgcct aacccatgtc gcgattaatg gcttgcactc tctgacagaa
     1141 aaatgctaca gaagccaaca tgacagatgc acgctttttt ggcggcttga tattaattgt
     1201 aaattttatt cgtcgttttt tgtgtttaca ttaattcgtt tgatttcctc tcttgaatca
     1261 agttgcccaa cttatgggtt gtatcgcatc atgctgtaaa ataccccccg tgcggggtgt
     1321 tatacggata tgatccgcat aacagaaatt gtatgcaaca ttatacagcc atcatcaggc
     1381 aggaacagag gaaattccct tcttatgcgt ttaaaagcag atctaactct tttggtcgtt
     1441 tcgattatct ggggctcggc ttttgtggcc cagcgtgtgg cgggtcagca ggggagcgtg
     1501 tacctgttca atggggcgcg ttatgtgctg gcggcgctgg tggtgctgcc ctttgtaaag
     1561 cgcgtgaatc tcacatccat gcctcatgat caatataaat ggatgttcat tgcaggtttc
     1621 tttttgttcg cgggtagtgc gcttcagcag gcaggcatgg tttacaccac ggcgggtaat
     1681 gcgggcttca tcactgcctt gtatgtggtg ctcgtgccgt ttgccctgtt tttcttctgg
     1741 cgcgaaaaac cgcactggat gtcggttgtg gcggtggttt tggccggggt gggggcattt
     1801 ctcctttcaa cgggtggcag gttcgaggtc cagaaaggag acgcgctgga agttgtcggc
     1861 gcattgttct ggaccttcca cgtcatcatc atcggaaagt ttgcggcgaa gttcgaagcg
     1921 atgtcatttt ctgtgggtca gcttttggtg tgcggcgtgt tgaatttggg cgtcggcctg
     1981 gtttcagaat cgatgatgac ctttaactgg cagctggcct ttgccattgg gtatacagcc
     2041 ttcttttcgc ttggcttgtg ctacaccttg caggtttggg cccagcagca taccccgccc
     2101 gccgatgccg ccttgatctt gagccttgaa tcggtgtttg cggtgctttc tggctggctg
     2161 ctattggatg aacgacttgc cgtcattcag atctttggtg cggtgttgat cttcattgcc
     2221 gtggtgctct ctcaattcaa ggaatggaca tcaggtaaaa ttgaccacga tcatcttgtt
     2281 gagggaagat aacgccatga ctgaagaaag aaacctgatc gaagaactcg acctctcgtt
     2341 ccatccgctc actcaaaaat tgtggcacga ctttgaaatg ttgttcggca ggcgcggcgc
     2401 gtgtggcggc tgttggtgta tgcactggaa attgcgcgga aaggaattca ccgaaagtgc
     2461 tggcgatacg gcccgtcaga tgcagaaatc cattgtcgat tcaaagaccg tccccggtct
     2521 gctggcgtat tccgaaggct accccatcgg ttggatcgca gtggaaccgc gcagcgccta
     2581 tccaaaactg gcccattcac gcactctcaa acctgtggac gatcaggaag tctggtcgat
     2641 cacttgtttc tttatcgaga aaaagcatcg tcacaaggga attactgttg aattgctaaa
     2701 agccgccgtg gattatgtga aaggacgggg cggtaaaatt gtggaaggct accccgtgga
     2761 tgcaaagcag gaagcccccg caactttcgt tttcacgggc acggcttccg cctttcacca
     2821 tgctggcttt gtagaggtcg cgcgcaattc gcccacccgt ccgatctttc gatttgtgat
     2881 cgaataatct tttctttgtt cttttcgtat cctgcataaa taacctcccc tcatcatcga
     2941 gcaaatcatt tgagcgttac ctttcaacct ccttcaaaat catttactgc gcgtgattat
     3001 caattcatcg cgctggtttc aattgtggtc gttgcgattt ccatcggttt ggtgatgctc
     3061 aacctcacgc taaaagatgg cggtgatttt tacgtccact ggtttgcagt gcgcgttttt
     3121 caaaacgaca ggctcgaccc ctacagcgga gaagtccccg cccgtgtgca gcaacaggtt
     3181 tatggaggtc ccgccgcaca aggggatgaa ccctacattc tggatacgcc ctttcacatt
     3241 ctcctgctct actttccgct cacgcttctt tccgacccgc tgctggcccg ggcgatattt
     3301 actttgatct tgcaactggc cttgttcgcg cttgccttca tcagtctgcg tctcaccgaa
     3361 tgggaagtgc cgatcatttt ctacgtcctg tttcccctgc tggctatttt caatttttat
     3421 acctttcaag cgatctacga agccagtccc gttctattgc ttgggctcac ctacgccgaa
     3481 attttgatag ccctccgtgc cgatctggac gaagtggcag gggctcttgc tgcggtctca
     3541 ctgtactatt gggaagtcgg tgcgccgttt cttgtccttg tggccttgcg ggtgtactac
     3601 gaaaagcgca ctcgcgtttt tgcagggttt gccatggcta gtctcattct gttggtgctt
     3661 tcattcatgg cctatcccaa ctggttcatt ccgtttttac gggccacggt caacaacatc
     3721 aaagcggatt ttggtttcaa cgtccattcc attttcatcc gcatctggcc tgcgtttgga
     3781 ggtacatttg catggctgtt cattggcttg ttggtggtgg ccctcggcta cgagtggagc
     3841 gctgcccgca gcggcgattt ccgccgtttt tattgggcgg cctgtctttc cattgcagcg
     3901 gctccattgc tgggctttcg caccgaaatg gaaaacctcg ccgtgctgat cattccgctg
     3961 gccctgatct tcgccatcgt ccatgaccgc tggtacaaga tcggtaactt cctcaccata
     4021 ttgttgatgc tgcttgttgg caccctgccc tgggcggttt ccctgttcct tgtcccccgt
     4081 tttggaatca aagccgaaga aggtttgttc ctgtttttgc ctcttttcac tttcattggc
     4141 ttatactgga ttcgctggtg ggcgatccgc ccgccgcgca ttttgagcga tattgccagc
     4201 cgcctctaac cccattccat gcctatgaac tcgcgcacct acttcttcct ctttctcttt
     4261 gggcttgctg ttcccgtctt cattgcccag ttccagccca tcccgggcta tctggattca
     4321 gattattact atgcaggcgg aattcaactc gccacaggca aaggcttcac agaaccctac
     4381 ctgtggaatt ttcttgatgg caccacgtca ctcccgcatc cctcgcatag ttattggatg
     4441 cccctggctt ccattgtctc tgctttgggc atgtggctga ccggtcaaac cacctttgct
     4501 tcggcgcgtt tgttcttttt cctgatcgcc gcccttgtcc ctccgttgac cgctgcgctg
     4561 gcttacgctt tttctcaaaa aaatgaactg gccattgcat cagggatgct ggccatcttc
     4621 tctgtttaca acgccccctt tgtcggcgtg acggataact ttggtctttt catgttgttt
     4681 ggcggcttgt atttcatcat cgccacgcag cttatgcaaa accccgaccg aatgcgcaat
     4741 tggctgtttc ttggcctgct ctctgggttg atggctttct cgcgcagtga tggtctgctc
     4801 tggctgccgc ttacatttct attcgcctta tggcgtgcca gaaaaatttc atccttcatc
     4861 cctcatccct catccctctt gttgattctg cttggctttc tcgccatcat gtcgccgtgg
     4921 tatctccgta acctgaattt gtacggtacg cccatggccc ccggtggcag ccgcgcactc
     4981 tggctcgaaa actacgacca gacctttatt tatccgccga ccctgttaac aaaagaatcg
     5041 ttcctcgccc aaggctggga aaaaattctc gacgaccgcc tctgggctct gaacatgaat
     5101 ctgcaaagcg gtttcgctgc gcatggcgga atcatattat ttccattcat cgtggctgga
     5161 atcatttatt atcgtaaaga tgatcgcgtc aaactggcgg cactcgcctg gttgattctt
     5221 ttcctggtga tgacctttct ctttcctttt gcaggtgcgc gcggggcatt tttccatgcc
     5281 ggggcggcgc ttcagcccat atggtggacc cttgctccgt tggggcttga gtcgatcctt
     5341 gtgtccctga gaaagcgcgg ctggggcaac gaccagaatc ggttcgtgtt tcgaagcgca
     5401 ctggtcatga tcgccatgat cctcactgtg tatgtcgtct atctgcgggt gttcacactc
     5461 ggctggggcg aaatcgacgc cgattacaag gctgtagatc aattccttgt tgagcatggc
     5521 gttggcgaag aggatattgt catggcacgc aatgcgcctg ggtattacct gctgacaggc
     5581 cgctcggcaa tttccattcc atacggcggc gagaatgcgc ttttggaagc tgccaaacaa
     5641 ttcgacgcct actatctggt gttggaacct gaggccgctc tcgacccgat caaggattta
     5701 tttcaaaatc ccaacagccg caatcggttt gtgtatctag gtgatgttga tggtgcgcgt
     5761 atttacaaac tcgaaactaa ataagatttc aaaccgtgat ctgttgatgc tggctgccgc
     5821 agtggtggtg gttacttcca tttatcttct cgcaagttat tttaattacg gcatcggctt
     5881 ccccttggac gattcatgga ttcaccaaac ctatgcccgc aaccttgccc aacgcggcga
     5941 atgggcattt cgccccgggg ttccctccgc aggttccact gcacctctgt ggtcggcttt
     6001 gcttgcgctt ggttttcttt tcagcctttc gccatatatc tggacatatc ttcttggcgc
     6061 attcaccctc tttgcattaa ccgttgtttg tgagtgggcc gtacgtaccc taatcgattc
     6121 ataccgtcca cacctgccat gggtcggaat tttctttgcc tttgaatggc attttgcctg
     6181 ggcggcaatg tctggcatgg aaaccctgtt gcacggattg atcgtcacaa cagttttggt
     6241 tttgctgatg acgaacacgc gtcgctactt aaccctgggc ctgctcaccg gtctatcggc
     6301 ctgggtccgc cccgatggtt taaccctgct cggccccgtc ctgatcatgg ttctgatcca
     6361 tgaacaggat acccgtccgc gtttgattgc tttgctgcgc tgtttgatcg gttttggttc
     6421 attattcgtt ccatacttgc ttttcaatct ggccattggc ggcactccca tgccgaacac
     6481 cttctacgcc aaacaggctg agtacgcctc ctggcagacc atttccattt tttcgcggct
     6541 tgggcaaatg tttctgcaat tgctggttgg accaagcctt gtgttgatcc cgggcgttat
     6601 gggttggctg gtcaaaagca ttaaacagaa aatgtggggg agccttgccg cgctggtttg
     6661 gtgtgcagga tattttgggt tgtacatttc ccgactccct gtctatcaac atgggcgtta
     6721 catcatgccc gccatgccca ttttctttct gttcggtctg cttgcctttg ccgatttcga
     6781 taaaagcagg ctcttcgaac gctatcattg gatcggtcaa accatctggc gagcaagcct
     6841 tgtcatgctg tccgttgctt ttatttttct tggcgcccaa tcctactcgc aggatgtggc
     6901 cttgatagaa agtgaaatgg ttgtaaccgc aaaatgggtc gccgccaatc ttccacccga
     6961 tgccctcatc gccgcccacg atattggcgc gcttggtttc ttcgacaccc acgcgctgat
     7021 cgatctggct gggttgattt cgcccgaggt catcccgttc attcgagacg agcccaaact
     7081 ggcaaattac ctcaaccagc agggtgccga ctatttaatc gccttttccg gcttttaccc
     7141 agccatgaca ggcagtctgg agaaggtttt tgtaacaaac agcgtagtca cccttaagta
     7201 cgatgaggag aatatggctg tctatctgtg gaaaacccct taatgggtta aaatatgaat
     7261 aagcaacaaa atcaaaaaaa gagagagaat gggccaggga tgaaccagat tacacttttg
     7321 atcgttgatg atcatccatt gtttcggcag ggagtggtcg atgccttgtc attggaaccc
     7381 gatatgcgca ttattgccca gtctgccgct ggtgatgaag cgctggagtt gatccgcacc
     7441 aaaaagccaa ccatcgctgt gatggatgtg aacctgcccg gcatgaacgg tcagcaaatc
     7501 acccatcagg catctcagga tagactgccc acccgtatta tcctcatgac aggatatgac
     7561 gacgtggaac aagccatcca tgccgcactg gcgggtgctc atgggtactg ctccaaagat
     7621 attgagcctc agagcctttc gcgcatcatc cgcgaggtgg ctgaaggaaa atatgttttt
     7681 gaaaataacg ttttcacccg tcgtgaattg gaactttggg tggaggacag gatcgaagga
     7741 gcccgtcgtt catacagtga acctggcact cccttccatc cattgtctga ccgagagatg
     7801 gaagtccttg cttgtgttgt tcgtggcatg agcaacaaag aaattgccac tctgttgggg
     7861 atcagccacc aaacggttaa gaatcatgtc actgccattt tgcgcaagtt tggcgttgaa
     7921 gatcgtaccc aggcggttgt gtatgccttg aagcgtgggt gggtaacctt gaaggatacc
     7981 aatattcacc cccaggagta ataaatgacc agtaccgagc ctatggattt tcagagcgaa
     8041 ttggatgaaa cacagcgtgc tcttcgcgaa gtgaccttga tgatcgaaca gagtcagggc
     8101 gaacttggca agatcaccca gcgaaatacg gcgatcactt cgcatcttca gcagttgcag
     8161 aaacagggga caactgttga tgatctgaaa atgtcatacg attcggcttt ggatgttcag
     8221 cagcggcttt ttgtaatgcg gggacaattg gaaaaactgc aaaacgataa aagccatctt
     8281 gagaaatata aaaccgtttt ggagaatatc gtttccgggg cagcttcagg cggagatgcc
     8341 gccggggcga ctcctgccgc aggtggcaaa agccagatgg caggtataga aatgatcgtc
     8401 aatgcgcagg aggctgaacg tcagcgcctt tcacgtcaaa tgcatgatgg tcccgcccaa
     8461 gccttgtcaa attttatttt acaaactgaa attgccatgc gtttgctgga tgttgaccct
     8521 gcacaggcca aggatgaatt gggcaatttg aaaacctcag ccatgggcac cttccaaagg
     8581 gtgcgcagct ttatttttga attgcgccca atgatgcttg atgaccttgg tttggtgccg
     8641 accctgcgaa aatatgccga tgcatttaaa gagcaggcca gtcttgatgt gagtgtgact
     8701 gtcactggca cagaacgtcg tttggaacct tatttggagg tcatgatttt ccgcgcggtg
     8761 caggaattgc ttggcaatgc ttcccgtcac agccaggcta ctttgatcaa ggttcaaatt
     8821 gaccttggga acgacacaat ccgtgtcagt gtggaagaca atggcaaggg tttttcacct
     8881 gaaatcctga aggaatccag caaccttggt ctgaaactaa tccgggagcg ggcggaaatg
     8941 ttaggtggta gcttcgaggt tgatagtgcg gccggttctg gtacaaggat tgcattttcc
     9001 gttccggcaa aggtgtaact tgaaaagaac gcatcccaat cgcctacaat attgttaggc
     9061 aattgggatg cgtggtactt gttttgtatg gtgggacaaa catataatac ggaatatttt
     9121 ctttagggga tgttcatttt gactgatctg ccatggcttg tatcgaagag aacgttcttc
     9181 ctgccgtaaa actggctcga aaaggcacga aattatgttt ttggcatttt tcttgtataa
     9241 tgaatatgtt ataaaatgct ctatcaaaga atgtgaaaag aggagagtcc tatgcgtcgc
     9301 ggaagaaccc tgatttttgt agtactaata ctaattatcg gtttggcggt ggcagtggcg
     9361 gcatactttc agtttctgag gccagcggct acacccacac agccagtaac aaccgttgat
     9421 gtatatatgg ctggtcagcc aattgcacag ggtagtgaaa tcacgcctga gttgcttact
     9481 acgatcaaaa ttgcacctga gaatctggtc tcggtaatgt ataccgtgga aatgcagtca
     9541 cagcttatcg gcaaagttgc caagtatcca ctggatcaag gtgtcattat tacccaggcc
     9601 atggtgagtg acgcaacctc ggctgttccg atctctggcc ctcaatgggc ggctttgatc
     9661 cccaccggaa tgacagcgat ctccatccct gcagatagat tgtctttagc cagctatggt
     9721 gttaacaatg gagcccatgt taatgtcaat gcctgtttct tgtttgtaga tattgaccca
     9781 tctttccaat ctgctttacc gaatcagacc ggtcaattaa ctggtaccgg ttttacgcct
     9841 gaaggattgc ctgtactttc acttggtgtt tctgccagtg gctccagtca gggtcgcctt
     9901 gaactcgacc catcgattca acagccctat tacttgatgc cgtcggaacg tcaacgtccg
     9961 cgtatggtgt gtcagatgtt gttgcaggat gttgtagtga tgaaagttgg tgatttccca
    10021 tctgacccgg ctgcgagtct ttctgcgccc gcacctgtcg caacagaccc ggagactcag
    10081 caagctgcgc cagttccacc tgatgttgtc acattgatcg tactccctca ggattctgtg
    10141 actctttcct atttgatcca cacagatgcg gaattgacct tgactttgag aaatccgacc
    10201 gatcaattgc gccaggcaac cgaggcttca actttgcaat tccttctgag ccaatacaat
    10261 atcccagttc ctgcgaaatt gccctactct cttgagccat atactgtaag tttttgtaat
    10321 ctcagtcttg gtacttcggc aatttgttct caaacagcag ctccgcccca gtagttacag
    10381 ttttttttta ctggagatca tatttgtatc ccgttgttgt tttaggcccg gcggcacaaa
    10441 aaggtagaaa aatatagaaa ttagtgagat tagacttgaa ctttcgaaat gaatggaagc
    10501 ataaacatgg ccgcagataa aattcgcatc ctcattgtag atgatattgc agagacgcga
    10561 gagaatattc gcaaactgtt gcagtttgag tcagacgttg atgttgttgg tgtggcgcga
    10621 tctggaaaag aaggtattca acttgctcag gagcttgatc cagatgttgt attaatggat
    10681 atcaacatgc cggatattga cggcattact gcaactgaac aggtgcgtaa gaaatcaccc
    10741 catgtgcaag tggtgattct gtctgtgcag aatgaccaaa gttatatgcg acgtgccatg
    10801 ttggctggcg cacgggattt tctaaacaag ccgcctttgg gcgatgaatt aatttccgct
    10861 attcgccgtg caggggaaat ggcacatacg gagcgggcaa agagcgccca gcagagtttt
    10921 gtaggagcgg ctgtggctgg ctctggcggg gctgcaatga attttgcccc ggtgcataag
    10981 ggtaagatca taaccttata tagtcctaaa ggtggaacag gatgcaccac tctggcggta
    11041 aacctggcga ttgcgttaaa caatgaagat actcgcacat ctcttgtaga cgcaaatctg
    11101 cagtttggcg atgtagccat ttttgtgaat gaacctggca agaatgcgat cattgaactc
    11161 gccccacggg ttgatgaatt ggaacccgag gtcgtggatg atatcctgat caatcatgga
    11221 gcttccgggg tgcggatttt gaccgcccca caacgccccg aatatgccga aaagatatcg
    11281 gctgagcaat ttaccaaggt tatccagtat ttacagcgga tgtatgcata cgtggttgta
    11341 gatacatcgc acattttgtc ggatgtcgtc ctgtctattt ttgatgtaag tgatgtcatc
    11401 gtgttggtga ctacgcagga gattccagcg atcaagaacg ccagattatt ccttgacctt
    11461 ctgcagacca tgggtattaa caagaatcgt gtcattttta caatgaatcg ttacgataaa
    11521 cgcatttcaa tcactcctga acgagtgagt gagaatctta agcacgaagt atctgttaca
    11581 atacctttgg atgaaaagat cgcgattact gctgttaatc gtggagtgcc atttatgctc
    11641 gataacaaat cccagcccat tggaaggggt atattctccc ttgcagaggc ggtccgaagt
    11701 cgtttgacaa ctttagaatc tgaaaatgat atgtctgata gacggtaagg agagttacta
    11761 tgtcgttgct tcgacgtatt gaacaggggc agcagggggg tggacagcaa cagggaaatg
    11821 gtaatacgag tgggactccc tcgccgacac ctccggcaaa gcagccaggt tcagggcagg
    11881 gtggagaagg ttctggcggt ggaggagata cctcccgcct gtcttcatta caggcaaggc
    11941 gtgtcagcgc tccgatcact tcccctcaag ctggatctta ttttgattta aagacccgag
    12001 ttcaaaacaa attactcgct gagatcgacc cgtccatgga tgtatccagg acggacgatg
    12061 ttaagcgtac cattcaggga ctgttcgagc agattcttac tgaagagaat atcgttcttt
    12121 cgagacctga acgcgcgcgt ttgtttgagc agattgcagc agaaatcctt ggtctgggtc
    12181 ctttacaggc cctgcttgag gacgacacca tcacagaaat catggtgaac ggccccaaaa
    12241 atatctatat tgaacgtaag ggtaaactcc atcgcgttcc ggtgaccttt gaaagcaacg
    12301 agcacgtgat gcgtatcatt gatcgtatcg tggccccgtt gggacgccgt atcgatgaat
    12361 cgagcccata tgtagatgct cgtttgcagg acggttctcg tgtaaatgca gtcatccctc
    12421 cgatttcctt gattggcccg gtgttgacca tccgtaaatt ctcaaagaat ccgatctcaa
    12481 ttgagcagat ggttcagttt ggttccattt cacatgaggc agtgcaattt ttgaaggctt
    12541 gtgtggaagc acgattgaac attgttattt ctggcggtac aggttctgga aagacaactt
    12601 tgttaaacgt tctctccagc tttattccag gagatgagcg tattctgacc attgaaaacg
    12661 ctgctgaatt gcagcttcgc caggaacatg ttgtaacctt ggaatcccgc ccgccaaaca
    12721 ttgaaggccg cggtgaaatt accattcgcg acctggtgat caatgccctg cgtatgcgcc
    12781 ctgaaagaat tatcgttggt gagtgccgtg gtggagaaac cctggacatg ttgcaggcca
    12841 tgaatacggg tcacgatggt tccatgacta ctgcccatgc aaactcgccg cgtgatgcga
    12901 tcgctcgtat cgaaacgatg tgtctcatgg ctggtatgga tctgcccgtt cgtgcgattc
    12961 gtgaacaaat cgcaggtgcg gtggatgtca tctgtcaaca ggctcgtatg cgtgacggta
    13021 gccgtaaagt aatcgccatt actgaagtaa gtggtatgga aggcgatgtt attaccatga
    13081 cagatatctt cacttttgaa caaactggca tggaaaacgg caggatcatc ggccgaaccc
    13141 gtcccaccgg gttacgtccc aaatttatgg ataagattga ggctgctggc attcatttgc
    13201 ccccatccat ttttggtatc ggtgaaagaa gaagatatta aaaatctgac gggttattaa
    13261 aaacctatgc ttaacataat tatcattggc ggtgttgtct tgatcattct gttgatcatt
    13321 ggcgtcgttg tttcttcaaa cagcgaacgc gcagtggtcg agcagcgtct tgctcagtac
    13381 ctggatgatg acaaacagga tattgacagt gcggctcaaa actctgtttt gacagagtgg
    13441 gtctcgaaaa gggtggaaaa aacatccttt ggtgaccgta tcgccagaga ccttgcccgt
    13501 gcggatctga agtttaaggc gggtgagtac tttattttga tcattgttag catcttgctg
    13561 ggcgggcttg tcatctggtt tttggggaat caacattggg cttctgctgt catcggcgct
    13621 gtagctggtg cctttgcccc gggaatctat gtaaggcgtc aacagaagca acggttgcag
    13681 aagtttaacg accaactttc agacatgctc aacttgatgg taaatggtct gcgggctggg
    13741 tattccacaa tgcaagccat ggaagcagtg agtaaggaac ttccatcgcc aattaacgac
    13801 gaattccgtc gcgtagtgca ggaaatgcaa atcggtattc ccatggaaac tgcattggat
    13861 aaccttttgc gccgcattcc aagcgatgac ctggactttg ttgtaacggc cattaatgtg
    13921 cagcgtgaag taggtggtaa tctttctgaa attttggata ccatctcatt caccatccgt
    13981 gaacgtgtcc gcatcaaggg cgaggtcagg gtgcttactg cacaggtgcg tacttcagga
    14041 tctgttctct ctcttattcc gtttttcctt gtattaatcc tttggttcct taaccctgag
    14101 tatattctgg ctgttacggc aggtggacct attattacgg cagccattat ttgtgtggtg
    14161 cttggtttga ttttcactgg ttactttatc atgatgaaga tcgctgatat cgaggtgtaa
    14221 ctatgggtac tattgttggg atcattctgg ggatcgttgt ggtaggtgga gtgattgcac
    14281 tcatcgtagt tgggatgcgt gcccgtcagg gaggtgggga cgaatctgac cccatattgt
    14341 cccgtttggc agaggcaacc cagcgcggtg agacggtatc ccttgaagat atcgagttac
    14401 aacaaccctt tattcaaagg gtgatcgtgc caatgatcga aaaggtgggt gaagtttcat
    14461 cccgcttcac accgcaaaag cttttgcagg agaccactct taaactggag ctggctggta
    14521 accctggtcg tattgatgct gcgacgttcc tggcgacccg cttttttggt gctggtatct
    14581 tcggtggtgg tctgttattg atcgctttgg cctctcaaac ctggccggtt ggtagaatca
    14641 ttttggtggt tgggttcttt actgttattg gtttcttctt cccacagttg tggttgcaga
    14701 gccgcatcaa tagccgtcag gcggaggtgc gcaaggcatt gccggacgcg cttgatctgt
    14761 taaccatttg cgtggaagca ggtctgggtt ttgatgcagc catgtccaaa gtggctgaaa
    14821 aatgggagaa cgaactctcg ctgatgtttg gacgttgtat tcgagaggtt cagcttggaa
    14881 agacccagcg cgaggcattg cgtgatatgg cagaccgcct tggcctgcct gagttgacca
    14941 gttttgtcgc cgccgtgatt caaagccaga tcctcggtgt gagtcttgcg aaggtgctgc
    15001 gtattcaatc ggaccagctg cgcgtcaaac gccgtcagtt cgcagaagaa ttggcacaca
    15061 aggctccagt aaaaatgatc attccgatgg ccttgttgac ttttccctcc atcatgatta
    15121 ttttgatggc tcccgccgcc ttccagatcg caggcgcttt tgggccattt ttaggtcagt
    15181 aaaaaaactt ccagcctttc ttttgcttgt tttgcaaacc tgcacaagca aaaaatggag
    15241 gcgcagtttg tctaatagat cattggcata taatctttac aggtcattat gggctggttt
    15301 ggacctcctg tatccaccgc aatgtggcgg atgtgggaaa actggctccc gttggtgtgc
    15361 ggaatgtcaa cagcgggtgc aggtcctgaa tggaaatctg tgtgaagtat gcggccttcc
    15421 ccgggaaagc gcaggcgtct gtgacacctg ccttgcggac aggccgcatt ttcattccct
    15481 gcgtgcatgg accatattcg aagacccgat gcgcaatgca ctgcataaat tgaaataccg
    15541 acgtgacctg gccctggggg atgcacttgc tgcacatatg tctgattttg tgcgcacctt
    15601 gaattggcag gccgatatga tcgttcccat tccattggga aaacagcgat atcgcgaacg
    15661 gggatataat caggttgcaa tgatcgcaaa accgctggca ttgtcgcttc gaatggaatt
    15721 tgcgccacag accctgagcc gtcgtaaaga gacacgctca caggtggggt tgaccaagga
    15781 agaacggcgt gtaaatgttc acgaagcgtt tcaagcgggg acgggggtaa aaggaaaaaa
    15841 tgtcattgtg atggatgatg tctctacaac aggatcaacc ctctcgtcca gtgccgaagc
    15901 gttattccgc tctggagcca gaaatgtatt tgcgttaaca gtcgctcgtg ccctgcctca
    15961 tcacggtctg aggcatgcat gagcgttttg tgttcgtaac ttttcatccc tataaggagg
    16021 acgtatgtcc aacaaagtgg aagtacagac acgcaacatc cgtttgaccg aaaaaataga
    16081 agagtatgtc aacaagaaag cgggtaatct tgaccgttac ctgcccacca ttgactcggc
    16141 ccgtgtagag cttgctcatc accaggctgc gcgcaatgcc accgaccgta atgtggctca
    16201 aattaccgta tatggaaagg gattcaccct tcgcacggaa gaacgtgccg acgaagccct
    16261 ggctgcattt gaccgcgcca ttgataatat gcagacccag atcgaacgct acaaagggaa
    16321 acaccagcat ggccgtggag atggacgctc tgctgcagag gccgccgaac ccatcattga
    16381 cgatgagacg ggtgaactct ctccgcttgt agcccgcaaa aagaaattcg tgttgtatcc
    16441 gatgaacgaa gaggaggctg tcgtccaaat gcgcaacctt ggacacgata acttctttat
    16501 cttttataat gctgaaacca gcaagatcaa cgttttatac cgccgtcgaa acgggagtta
    16561 tggattgatc gagccagagc tcggctaatc ggtttaccaa taactggctt ggcggctatg
    16621 ctgccaggcc agttttaatt tatcaaccat cggcttgaaa aaacgatggt cggagtgaaa
    16681 atggaatttg atgatgtgaa cgagagcgat gttgaaggat cggccattga caatgccgca
    16741 atagaaaccg ccgtgactca gattttgaat gctgtgggcg aagacccgca gcgcgaaggt
    16801 ctgcaattca ccccgcgccg cgtggcgcgc atgtatcacg aactgcttgg cggctacaaa
    16861 atggacccgg ttgccatcat caacggcgca ttgttcaatg tgaaatacga cgaaatggtg
    16921 ctggtgcgcg atattgagtt ttacagcctg tgcgagcatc acatgctgcc tttcatgggc
    16981 cgtgtgcatg ttgcctatat tcccgatggc aaagtgcttg gtctttcaaa gatcccccgc
    17041 attgttgaaa tgtactcacg ccgcctgcaa gtgcaggaac gcatgacacg tcagatcgca
    17101 gattttttgc gcgacctgct caaaccacag ggcgtggccg tcgtcgtgga ggccatgcac
    17161 ctgtgctcca tgatgcgcgg cgtaaaaaaa cacgacgccc gcatgaccac ctctgccatg
    17221 catggcgcat ttcgcgccaa cctcgccaca cgccaggaat ttcttgcaaa catctcacgc
    17281 ggctcggcac ccttacagct ttagaaaaaa cgcggcctca acaacaaaaa atgcggaaat
    17341 cggtttccgc atttttttat tttcatatag gggatgaaaa cccgaatcag acttaacctc
    17401 agcctgcttc tgtcattcgg catggaccgc cgccgaatca gtttcggcct ttggcgaaga
    17461 tttgaacctt gcttcgagca acataaagca gaccgcaagc gccagccatg caacgacctg
    17521 ctcctgccgc accccgttga agataagcgt gctatcgccg cgaaatgcct gtaaaaaaag
    17581 ctgcgaggca gagatcagcg cggcaaaggt gaggaaatga atgcctgggc gctggtcatg
    17641 tttttgacgc cagatcaggc caaagattaa gagcgaagcg agcgtctcgt agatttgagc
    17701 cgggtggcgg ttggcgctcc ataattcgat gctccaaggc acatcggttg cctttccaaa
    17761 agccgtacct gctgcgagat gcgaaaggcc cattccgacg gcgagggttg caaagaatgg
    17821 ggtcaatgca tcgagggtgg gccagagtaa aagtccgcgc cgttgaccga agatggtcgc
    17881 cgcaatgact gcggccgcca atccgccgaa agcgtcgaag atatcggggt tgatggagac
    17941 aatgccgctg gggcttttta gaaatgtggg caggttttgc aggatgaacg aaacccgacc
    18001 accgatgacg aaagccagca agccatagaa gatgacatta ttgaggtcgt ctttttggat
    18061 gccgtggcgc tcggcgcgct tttcggcgaa ggataggccg aaccatgcgg cggcaaccaa
    18121 caggatcatg tggcggggcg gggaaaatag attgcggaag agttcaatca tgttatttta
    18181 gaatctgttc gatacgggtg cgcagcaggg cttcagacat ggggccgccg atgacgacct
    18241 ctgagatgat gccttcacgg ttgatgaagt aggaagatgg cagggaacgc agttgatacg
    18301 cggctgcgac atcacccgtc tcttcaagca ggatggggaa ggtgaggttg tgttcctgcg
    18361 tgaatggaac cacttcgaag gggtcgtcct ggtaggtcat atctaccgcg aggacaatga
    18421 agccctgatc tttgtattcc tgatacatgg cttcgatggt gggcatttca gcacggcagg
    18481 gcggacacca ggtagcccag agattgacga gtacggcttg tccgcgcaat tcagagaggg
    18541 tgtaagactc gcctgtgggg ggttttaatg agaaatcggg tgcggaaaaa cccgcctgtg
    18601 gagcggggat gttccctgat gtggatgtgc ctgccttatc tgtgctgagc gaagtccatg
    18661 ctgcgcctgc gatgagcagg aggacgtaaa gaatgatgcg ttgagttttt tgcatgatgt
    18721 ctgagtatac atgaattggg gtgtaaaata gaccagtcgg ttaatctgtc tacttgattc
    18781 ttttataact tacccatgct attcactgac tctgttttcg ttggcgttga ccctacttcg
    18841 ggacgtaaat ctttcaccta tgccgcattg gacaaggatt tacgtctggt tgcgcttgcc
    18901 gatggtgaac tggaagatgt gaccacattc cttgctgggc agaaatcagc aactgtggcg
    18961 gttaatgccc ctgctggggt caatcgcggg ttggtgcgtg ccagaatgaa aaaggaaatg
    19021 ctgacgccgc atcaggtccg cggtgctgat attcgtctgg cggaatacca gttacgggag
    19081 catggaattg ttgttacggg tacggctgca agcgcggcgc tttgtcctgc gtggatgcaa
    19141 tcgggttttg agttgtatcg taagttgggg aaggctgggt tcaaaaagtt ccctgaaaaa
    19201 gaagcgcaat tccaaatttt ggaaacacat ccacatgcct gttattgtgt gctggcaggg
    19261 cagattccgc ttgccaaacc gtccttggaa ggccgcctgc agcggcagtt gatattgtac
    19321 gaaaatgggg tgtatgtcaa agacccgatg gacttttttg aggagatcac ccgctacaaa
    19381 ctcatcaaag ggatctggcc gatggagttg ttgtacctgc cagaacagct tgatgccctg
    19441 gttgcggcct ttacagcatg gctggctgtg aacaaacccg agcggatttc aacgatcggc
    19501 gatgaaaaag aagggctgat cgttctgcct gaaaaagaat tgaaggaaaa atattaggtt
    19561 ttgcaaaaaa acttgagctt aaacagcaga gctacagata cattctgtag ctctgttttg
    19621 tttgcggtca aaaactattt aattcccaaa aattttcttg ggttggtata ggcgatctgt
    19681 ctgtacagat cttcaccgag cctgctcctc agtaaaatgg acgcttcctt ttccgagata
    19741 cgttcctgct ccaccatgta gtaatcgctg ccgaagagta catgctcccg aatttgtttg
    19801 ttcgaaagca ggaccttgag ataatccacg aggtcaatgt aaagcccgtt caccttgggc
    19861 atgaagatgg tgtaggaaat atcggtgtag aggtttgggt aatttccgct ggtaagcata
    19921 tcagaaatga cccgtaccca ggcgcgcccg tctccggtgg atggcgtctg cccttttagg
    19981 tgttttaccc actcctctgc gccgccaaag tgagcaaggc acaaccgcag ttttttattt
    20041 tccttcaaaa cagggatgta gttttcagga tgcccaaagt tgattcggtc agattctgaa
    20101 agcccgtatt gccagattcc gctgggagag caatgcgaaa tgaccgggta ggtatcgccg
    20161 cagattttgt acacatccat gaggcgttca tgattcggtt tgtatccgag gttggggtag
    20221 atcttgatgc cgcggaattt gtcttttgca agattggctt tgacccgttc cacgatatct
    20281 tcatggcgcg ggtcaacggc ataaaatgga ataatacttc cctctgaagc tgcggcaagt
    20341 ttcagcaagt cggcgtgttg gtcttcaatt gactgtttca ttgcccccag gttcatgaac
    20401 tccatatcca tgggaaggat gacaaacttt gcattttcgg ggtattggtg tttgatttgc
    20461 ttgaaaatgg tttcctgcga gtcagatgct gaggtgttga ggaaacgcgc ataccgctcc
    20521 agcaggtcat cagaggctgg gttgatggcc gccagcaaag ctgcaaggcg gttgatcgcc
    20581 tttttcttca ccttctgcat tctgccgttt tgatctgcgg aatttctttc cgctccgcct
    20641 ctgatgtaat ccagaataac catgaacagg atcagcggcg agaaagcgat caacaggaat
    20701 ggcaacaaca gaacaaatac aaccttgagc gggctcatct ttccgctgtg tgagtcggtt
    20761 agtaacagtt tgtagaattg cccgggcgta ttcttttgcg tgaatgtgtg gatgtggcag
    20821 ttatagatgt tccataactg ctcttgcgga aggctgtttt cctgtttcat ggggcctcct
    20881 ttatttggat ctttctgagg tgtggattgt gtttactgtt tattcatctt cgtttttatg
    20941 ttcaacgaga atttcttcga tctttccaac acggtgtaaa agttcttcaa tttccagttc
    21001 ggctttgagg ttaacacgat aatcgagctc gctcctcaaa cggtctttcg catcctgacg
    21061 gttttggctc atcataatga ccggtgcctg aattgccgcc agcatggaaa ggaatagatt
    21121 aagcagaata aacggatacg gatcccaggg ctgtttaagt acagaattga tgccagtgta
    21181 cacaattaaa acaacggcaa atgtgatgac aaatccccat gaaccgccaa atctggcaac
    21241 tgcgtcagcc attctttcgc caaaagtttc atggtcctcg atctcttcat tcgggttttt
    21301 ggaaacgcgg gtgcgcatca actcatgggc cacgcgtaac tgtttttcca cgatggtcaa
    21361 catatccagc ccggccatgg gtttgacgtg taaaagggtc ataatgtcat ttcggtcaat
    21421 ttctatggcg gtggtgtctt cggtcgcgac agcagtggtt tggtgatttt cgccagccag
    21481 cagtgacgac attccgacca gtccgccgct atctgccaca tctacagtga catcctcgaa
    21541 attggcatct ttgatcgtga catgtatcag acccttctgt accagataag ccaacccgcc
    21601 ggggtcacca gctttaaata cggtctgccc ctctttgaac tcgcggacag aaacctgatc
    21661 tgcaagtacc tttctttctt catcatcgag taattcgaaa atgggaactt cattgaagaa
    21721 atttggatca accatgacag gcaacctcct ttttgattta gttaaagcat atcatcgttt
    21781 ggtaaaatag tgaaaaatct tttttacagg acttaatcat gactgctgat ttgcaagctc
    21841 gtgcaattaa tacccttcgt ttcctctctg ccgatggcgt gcagaaggcc aactctggtc
    21901 atcccggcct gccaatgggg actgcggcaa tggcgtatac catttggacc cgccacctgc
    21961 ggcacaatcc acgcaaccca aagtgggcgg gacgtgatcg cttcatcctt tcgggtgggc
    22021 atggttccat gctcctttat tctttgttgc atctgacggg gtatgacctg ccgctctccg
    22081 agttgcaaag tttccgtcag tggggcagcc ttaccccagg ccaccccgaa tacggcctga
    22141 cccccggtgt ggaaatgacc acgggaccgc tcgggcaggg atttgcgaca ggggttggca
    22201 tggcaatcgc tgcttcgcac cttgctgcca acttcaacca gcgtggacac gatgtgattg
    22261 acccattcat ctatgccatc gtcaccgatg gcgacctgat ggaaggtgtg gcctctgagg
    22321 ctgcgtcgct tgcggggcat ttgtcacttg ggcggttgat ctatctgtac gatgacaatc
    22381 acatttccat tgacggctca accgatctgg ctttcactga agatcgtgcg gtacgtttcc
    22441 gctcgtacgg ttggcaggtt ctgcgtgtgg aagatggcaa cgatgtggaa gccattgata
    22501 aagccattca ggaagcgaaa gccgaatctc gcccgacctt gatcatgtgt cgtacaacca
    22561 ttggctttgg cgcgccaaac aggcagggaa cttccaaagc gcatggtgaa ccgcttggcg
    22621 acgatgaatt gaatgctgcg aaagacaacc ttggctggcc gaaggaacca cgtttcttca
    22681 ttcccgatga tgtgctggcg ttttatcgca aagccgtaga caagggccgt gaactcgaac
    22741 atgactggaa gatgcgcttc gaggcatatg cccgtctgca ccctgcactg ggtgcggaat
    22801 tgcagcgccg tttgaacggc gaattgccct ctgattggaa atcggccctg ccgagtttcc
    22861 ctgctgatgc caaaggcatg gccacccgtg cggcctctgg caagaccatc aacgccattg
    22921 cggcgaaact gcccgaactc attggcggct ctgcagattt agccccctca aacaacacca
    22981 aaattgacgg aagccctgcc ttccagaaag attcgtatca ggggcgcaac ttccattttg
    23041 gcgtgcgcga acatgcgatg ggtgcggcgt tgaacggcat ggcggtcttt ggcggcgtga
    23101 ttccctatgg aggcaccttc cttgtatttg ccgattatgt gcgtccatcc gttcgtttgg
    23161 cggcgctttc gcatattccg tccattttta ttttcaccca tgatagtgtc ggtcttggtg
    23221 aggatggtcc aacacatcaa cccattgagc acctgacctc gctgcgcttg atcccgaatc
    23281 ttgtggtcat tcgccctgca gatgcaaatg agactgtcgc cgcctggaag gttgccattg
    23341 aaaggcgcaa cggcccaacc gtccttgcgt tgacccgaca aaatgtcccg actttggaaa
    23401 cctctgcaca ggttgaaaaa ggtgcttacg ttttgaagga ttttggaaat ccagaaatga
    23461 ttctgatggc ttcaggctcc gaggttggtt tggtccttga ggctgctcaa aaacttgcgg
    23521 atgagggcaa aggtgtgcgc gtggtttcct tcccaagttg ggaactgttc gagaaacagg
    23581 atgaggcgta tcgcgagtcg gtgctgccga atgccatcca gaagagactc gctgtcgaag
    23641 caggatcagg cctcggctgg gaacgatacg cgaagtccgt catcagcatt gaccgatacg
    23701 gcgcatctgc tccggcgaag gtcatcttcg agaagcttgg ttttactgtt gaaaatgtta
    23761 ttgcgaaggc aaaaacgttg taacgttcca acattacaac gttggacaaa ggataattat
    23821 gaaagtagca gttggttgtg accacggcgg atttccgctg aaagaagttg tgatcgaagc
    23881 agtgaaggct gccgggcacg aagtgatcga tgtcggcact tatagcgtgg atgcagtgga
    23941 tttccctgat ttcacaaaaa aggttggcga aaaaattcaa agcggcgaag ccgaacgcgg
    24001 tgtggtgatc tgcggttcag gcatcggagc agcgattgcg gccaataaaa tgaaaggcat
    24061 ctacgcctca atctgtcacg atacctattc tgcggcgcag ggtgttcagc atgatgcgat
    24121 gaacgttctg tgcctcggcg ggcgtgtgat cggcccagag ttggtaaaag tcatcgtgcc
    24181 cgcattttta ggcgcgaaat atctcggtga cgaaaaaggc ggcgaacgat ttgcgaagcg
    24241 tgtgaataag atcaaagagt tggaaggttg aaacgttgaa ggttacaggt ttcaaacctt
    24301 taaccttcaa cgttcaactt gtcaacattt aaacctgaaa aaaactggaa ccctaaaatg
    24361 aacccaatcc caaaacttac atccctcggt caatc
//
LOCUS       DAOQJC010000057        24357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_906_length_24357_cov_8.667805, whole genome shotgun sequence.
ACCESSION   DAOQJC010000057 DAOQJC010000000
VERSION     DAOQJC010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 24357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24357
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_906_length_24357_cov_8.667805"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..2323
                     /locus_tag="PLE39_10280"
     CDS             59..2323
                     /locus_tag="PLE39_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68758.1"
                     /translation="MSDFNPSPEFEEKIRKATDVPGARPEFVNKLRNELARRPVKMKP
                     RFILKPAWAFAFVIVALALIASAPAAVNALKKLFGYVPGVGLVDNSSGIRVLAEPASV
                     TREGVTLTVTQVLVYPDHVQLIYEVSGIATENDGNFALDATQNFTAFCGSPDGDAKLR
                     LPDGTIIERAFDTEKYPENIFATKPAYETVIPADMMEMTMVLKCLPWARLGAVPENWE
                     VPLKLKYVPAGETIGEPVLDVTPSASSAASDQGLSVTLDKVVPQDDLYSFYLTIAPEQ
                     ADESVLAYFPTYATLVDATGQKISLVYMHPFSGSHDVAEPWELQSITRPAYGPYTLVL
                     DSVATYYNEDGSHFFDVDLGPNPQVGQTWEINQTLNLAGTDVRVLSATMAERDLSMWG
                     GAANTQGLVFTFEAVDGKTPFSLDIMDRDVDHIMNGSIFPFSDGLTNTSEQYVTGLFY
                     EKGIPSGKLSIAVNGQTTMTNGHWEFQWASPDQTGEELLSASESISSAAGSSGVTTEL
                     RRVVKTNDGYLFFVHMTTPEQNPDFRVVEPVEVSVVDSTGKKIKLNLDGPQVYSARDE
                     NLWQFSTAEQVAAGPLKLVVDKAKVYYSKFNFATPPPADVYQNLVKENSFIFDAGADP
                     QVGQEWMLDQEFEAGGYHGIVKSVRAVTVDSQMLPFPELQPDASLNRGYEFTVEAMDP
                     SIQWNVSLFVSKPEGDPGFADCIGGMDGEAGSVTTHTMSCRGLSSGILQVEISEISIW
                     LNEIWEVAWVMPGQ"
     gene            2407..2772
                     /locus_tag="PLE39_10285"
     CDS             2407..2772
                     /locus_tag="PLE39_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68759.1"
                     /translation="MNKYIKTLLVVMIFGLSACGQSRTTPQQDVFLDPPAIVVKASRE
                     NCPSIVAEVGMTIAWENVDTVSLPIKIEFLDENGKVTDTGLSVIDPETVFSTSFPEAG
                     VYYVYCTENADIPSTITVK"
     gene            complement(2820..4637)
                     /gene="argS"
                     /locus_tag="PLE39_10290"
     CDS             complement(2820..4637)
                     /gene="argS"
                     /locus_tag="PLE39_10290"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNE68760.1"
                     /translation="MFTKEQQLIEEKIKAYCQANDIALAELKWQPIPFSGEWGFATSF
                     FQTAANEVKLGKGNKLPVPQKAQEIAEQVRGQIGSVDGISHIEAVKGYLNVYFKTSDY
                     AQRVVDEVLASGLNFGRGAAKNERVMVEYAQPNTHHSFHIGHARNTILGEVLARLVEF
                     AGFDTIRASYPGDLGLGVITVMWIYEKFYKGQEPTGIHERGQWLAKLYVEATALLEKK
                     ENETPEQTAQREAYEAERREMYRKWDAGDEAVRKLWHETREWSLEELRDVLRMLDVKM
                     DVWFYESEVDEPSKAIVEELIAKNIADDERPQGGAVIVKIDEKLGLTKEKYRTNVILR
                     RDGTTLYLTKDLALAKVKFEQYHVDRSIYVVDVRQSLHLQQAFAILKLWGFPQSEKCY
                     HLGYGFVSLPEGAMSARRGRVVLFKDVADEAVKRVLSVETEKSADIPAEEREKVAYQI
                     GMGALVYSMLSVDNNKDIVFDINEALSFDGRTGPYIQNAHVRANSILKKAGVSTFERS
                     NVQTFNYDFTKHEIELIEQISRFPKAVQQAAEEYRPLVMASYAYELANTFHSFYHAVN
                     VMQTEDENTKNARLRLVAAAKQTIANALRLLDIQAPDVM"
     gene            complement(4744..5028)
                     /gene="rpsT"
                     /locus_tag="PLE39_10295"
     CDS             complement(4744..5028)
                     /gene="rpsT"
                     /locus_tag="PLE39_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNE68761.1"
                     /translation="MANINSQIKRNRQNEKRRIHNRNFRGAARTAITAARSSLAKSAP
                     ETQEDIMLAVSALDKAAQKGVIHPNNASRRKGRLMKKLAAFLSAKSEKKA"
     gene            complement(5120..5944)
                     /locus_tag="PLE39_10300"
     CDS             complement(5120..5944)
                     /locus_tag="PLE39_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE68762.1"
                     /translation="MNNRPPISSSPNPGTISLLRKRKRNPNIVLIAAGVLVLGGIIML
                     VVWLTGDSSPLNALLATETPTPTMTFTPTSTSTPTSTATATETPTITPTPTFSTIFNY
                     TVEAGDSLFSIVEKFNLGENGVALILLLNPYGGIADSGFPIGVDPATLNILPGQSIVL
                     PYPGMPLPTATPLPANLAPGTKIEYIIQSGDSLGSIAAAFNSTIDEIIAANGITDPNA
                     IKVGDTLQIPVNVVTATATRPPTSTPITPGPGTLLPTVTFTPINALPATATATPTP"
     gene            complement(6120..8996)
                     /gene="uvrA"
                     /locus_tag="PLE39_10305"
     CDS             complement(6120..8996)
                     /gene="uvrA"
                     /locus_tag="PLE39_10305"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNE68763.1"
                     /translation="MSTNVIRVVGARVHNLKNITVEIPRDKFVVITGLSGSGKSSLAF
                     DTIFAEGQRRYVESLSAYARQFIGQMDKPDVDYIDGLSPAVSIDQKSTSHNPRSTVGT
                     VTEIYDYMRLLFARAGIPHCPICGREVVKQSAQEIVDKIEKLPEGSRVLILAPLVRAR
                     KGTYQAVFEEIRKAGFTRARVDGNTHSLDEEIELDRYKQHTIEAVVDRLVISQEGDKE
                     EKKSARTRLTDSVETALKFGEGYITVNLVDKKEDIQFSEHLACPEHGVSISEIEPRTF
                     SFNTPQGACPDCQGLGSKLEIDPERIIPDPDRSLNEGAISSMEWSGPKEEGGYYWQGL
                     EAAAHAYKIDLNKPVRELTKDQMKIILYGTGEKQIQMNYKNSNGNEFKFTRAFEGVIT
                     NLERRYRETNSEWIREKISEFMSDRPCPTCNGKRLNPAALAVTVDSVNIVEANSWPVL
                     KTLDWVKKISGKGSPLTSKQKTIAERVIKEIHERLNFLVNVGLDYLTLNRSAATLSGG
                     EAQRIRLATQVGSRLVGVLYVLDEPSIGLHPRDNSRLLETLKGLRDLGNTVLVVEHDD
                     ETIRESDWIIDLGPAAGEHGGEVIAEGTPKQILAHPKSLTGAYLSGRKQIEVPKKRRE
                     GNGKALKIVNATANNLKGINVSIPLGKMVCITGVSGSGKSTLMSDILYNSLAAKLMSA
                     RTSAGEHERIEGIEHLDKIINIDQSPIGRTPRSNPGTYTGLFDEIRKLFAELPESKVR
                     GYKPGRFSFNVHGGRCEACQGQGELRIEMQFLPDVYVPCDVCHGKRFNRETLQVLFHD
                     KYSISDILDMTVDHALDEFKNYPPMQSKLKLLQEVGLGYVRVGQPATTLSGGEAQRVK
                     LSKELSRRATGRTLYVLDEPSVGLHTADVHKLIEVLQRLVDSGNSVVIIEHNLDIIKI
                     ADWLIDLGPEGGDRGGQLLAEGTPEQVMKVKESYTGKYLKAHVK"
     gene            9172..9549
                     /locus_tag="PLE39_10310"
     CDS             9172..9549
                     /locus_tag="PLE39_10310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE68764.1"
                     /translation="MEYNEEEILLRASQGDRDSFGQLYAHYVERIFNYVYYRTGNTHD
                     AEDLTARVFQRAMKHIVNYTDRGVPFSAWLYRIAHNLVANWHRDRSRKQEIPLDDLPI
                     LPTKGDHPERNLVRSQEQDVLLK"
     gene            9546..9734
                     /locus_tag="PLE39_10315"
     CDS             9546..9734
                     /locus_tag="PLE39_10315"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNE68765.1"
                     /translation="MIRTLPAERQNLLILKFVENMSNAEIGAIMGRSEGAVKSLYHRT
                     LLALRDQLEDQNLNIEGE"
     gene            9738..10580
                     /locus_tag="PLE39_10320"
     CDS             9738..10580
                     /locus_tag="PLE39_10320"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNE68766.1"
                     /translation="MLRIVTDGAADILPAWGQEYGIEVIPVNILFGEKSYLQGVELDN
                     EGFYKLVDDTKRIPKTSQPSPHQFVEFYRKIAQKGDTILSIHITAKLSGTYASAIAAA
                     EELKDEFKVIPIDSAVGSLGIGMMCREARRLERAGKTVDEIVSYLEDIKYKIRVILTL
                     DTLEYAKMSGRVKTLQAALASLLNVKPIAILRNGDLNMAERVRTRKASLDRVIEIAEE
                     EFGKKPVYLAVVHARDAKSGQGLLDDAKSHFNHKETMLSDLSISIAANLGPGTVGLIL
                     YPAE"
     gene            10596..10865
                     /locus_tag="PLE39_10325"
     CDS             10596..10865
                     /locus_tag="PLE39_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68767.1"
                     /translation="MMTRRNTSTTLEDLDTLEAHLAGTLKPISPPKELVQRLGARIRV
                     PSRSEITLRFTDWRRMFFVVGGVVSGMLLLITVARALYYLAGRRM"
     gene            complement(10989..11318)
                     /locus_tag="PLE39_10330"
     CDS             complement(10989..11318)
                     /locus_tag="PLE39_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68768.1"
                     /translation="MDNVTTQLQKEFEKAQQARIAKNEGQARVCARRAAGVAIRDYLT
                     RKGDKIPSMSSYDLLNLLKDNAALPPDLKLAVDNLTVRVTEEFTLPVDADLIAEARKL
                     CNWLLSN"
     gene            11388..13877
                     /locus_tag="PLE39_10335"
     CDS             11388..13877
                     /locus_tag="PLE39_10335"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNE68769.1"
                     /translation="MKPTLFRIFGYLIIVSLALAACTPTAVPTEAVEEGDFLGAMSQP
                     YELTGSYTVTNDFVLATYYVENAVALTDMHGFVLRDLEWEIPVDSQVLGFMTFDADAL
                     GGTYELSLPAIPQGEFNDVDNNGAADAGVQIFAVAYSPNVYGGPFAEGDDRSRGWPSY
                     LASVKTDSENKDEVIGGKLVVWAEDANQQFPTSFGDDGLLFTADDPVAPISQGYSIVD
                     LDQVPFAVTQDAISDLPLYEPEDIATKDYSTLSYTEAFDKMFEVIRNEYAFADIPGEA
                     PDWDALYAELKPRVEQAEKTSDPNAFYLALRDLTWAFDDGHVSLNGGQYFQQDFATAV
                     SGGYGFAIRELDDGRVIVIFVMDGGPAQAAGIQVGAEVTEFNGLPISDAISGAQSYSN
                     QSSEFAIRYQKSRYLLRALPGTEATVKFANADGIPQVVTLTAIEERQSFSRTSLYFGV
                     DNSSLLPVDLKIIPVNNDNVGYIRINSNYDDLNLVIRLFERALKQFEANEVAGIIIDM
                     RYNNGGAPLGLAGFFTEQEIPLGQLEYYSDKTGKFEPEGLRQKFLPNQNQYRFEKTVL
                     LVGPACFSACEIEAYGFSQVPGMVVVGLTPTSGVEAETARGTFNLPEGFSVTVPTGRF
                     TLPDGSIFLEGQGVPPTLRIPINEETATAIEDIVLQAGIDAVLQPFGAGVTPSAAPKV
                     ASQTEAESALSSGAPFLEDLARESYDPAITFAQPGTATYSISMTDPEPVIWGYSWCAV
                     DAATVASNFENIDMQFMLDGKEVPTDSFGTLELETGGKVCRLIYTSLSEWTSGEHHLS
                     TTATFTAAINDGSANFEAGEYILEYTVYVKP"
     gene            13934..14680
                     /locus_tag="PLE39_10340"
     CDS             13934..14680
                     /locus_tag="PLE39_10340"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNE68770.1"
                     /translation="MINIFMKSPLPNTLAVIVLLALIITWVTSIVLSMRDVPVETEKG
                     WYANLLPIFSILGIPAVLDLLQTKGVTFVFAAIVFTVLLLNIVVPILKIYGKSSHFLV
                     ADWYKWSVLISTVGGLAVAGYLTFVEATNAPVVCGPSDGCAAVQNSKYAVLFGVLPVG
                     VLGLAGYVGILAGWLVLHFGPDAIKRMAALAVWAMCIFGVLFEIYLTFLEPFVIGSTC
                     MWCISSAVLMIILLLVSTPAAQQAFVINDD"
     gene            14843..15379
                     /locus_tag="PLE39_10345"
     CDS             14843..15379
                     /locus_tag="PLE39_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68771.1"
                     /translation="MKTMFKKTILVAIAAALVLAALPFSSAFAMNGSAEGEVTNEKLE
                     QMWARQLQAYERVGKGFEKTDEFTAKVQDRIDKAAANGKDITALQAALDAYEAAVKNA
                     RPAYESIQGIVSAHQGFDANGKVTDAEKAKATVQAMRTKMQEVKTSMNGTFKALRDAI
                     KAFREANQPAEGTSERGT"
     gene            15584..15967
                     /locus_tag="PLE39_10350"
     CDS             15584..15967
                     /locus_tag="PLE39_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNE68772.1"
                     /translation="MKKKVIQTEKAPKAIGPYSQAIRTDAFIFTAGQIGIDPTTGELI
                     SGAVEEQTRQALSNLRNVIEAAGSNMDNVVKTTVFLKDMADFPKMNAIYAEFFGENPP
                     ARSTIAVAALPKGGLVEVEAVVLAV"
     gene            15986..16666
                     /locus_tag="PLE39_10355"
     CDS             15986..16666
                     /locus_tag="PLE39_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE68773.1"
                     /translation="MASELILLVDDEPSIIQLSRMYFERDGFRIQEVGDGEAALEAVT
                     KHHPALIVLDVMLPKLDGFEVCRTLRAKGDQTPIIMLTARDEDIDKILGLELGADDYL
                     TKPFNPRELIARVKAILRRSDGKKQADGKPLHRGDLTIDPVSREVSIASQILDLRTQE
                     FDLLLTLAEQPGRVFTREQLLQLAWGFDFYGQTRTVDVHIAHLRKKLEGSNIKIETVT
                     GVGYKLVV"
     gene            16700..18079
                     /locus_tag="PLE39_10360"
     CDS             16700..18079
                     /locus_tag="PLE39_10360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE68774.1"
                     /translation="MFSSLRSRLWLSYALLIVTALGVVAIILFTYLLTNPLLYRQTLE
                     RLRAVQGVVLDNSRLPNAQPISIAAQKASLTFDVRIVQYSREKQVLSDTYSSKEPVLP
                     FPEKRVFARNVPLVRDENGQAWLYSVEILPDKSYLLVAAPRPKLSISNVFTDDFLPLI
                     FRSGLLALLLSLVVAFFFARWIADPLQKVVAAARAMPSAEIKPVEIGGPHEVQELTRA
                     FNSMVARTQASQRSQRDFVANVSHELKTPLTSIQGFAQAMLDGTADSEEARQKAAQVI
                     YDESGRMHRMVLDLLDLARLDSGTADITMSPVKMSALLNAVAEKFAPQSTRTGVRIQV
                     EAAADLPSITADGDRLAQVFTNLVDNALKFTPSGGSILLQASMLDGEMRVAVTDTGAG
                     IPAEAQKHIFDRFYQADPARKGGAKHGAGLGLAIASEIVQAHGGRISVRSRQGEGATF
                     EVFLPLVRK"
     gene            18106..19116
                     /locus_tag="PLE39_10365"
     CDS             18106..19116
                     /locus_tag="PLE39_10365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE68775.1"
                     /translation="MPPFVSIIVPCYNEEATIRHLLNAVFAQTYPRAQMELIISDGMS
                     TDRTREVVEAFQKEHADLEIRVVNNTARAIPSALNQAIREGRGDVFVRLDAHSMPIPE
                     YVERCVAAHEAGKGENIGGVWEIRPGADTAIAKAISFAAAHPLGVGDAMYRLNASAGA
                     VDTVPFGSFRRALIEKIGLFDETLLANEDYEFNTRVRESGGTVWLDPSIRSVYFSRST
                     LGSLSAQYWRYGFWKLKMLKRYPHTLRWRQALPPVFVSSLIFFIVLSLFFGLAKYALA
                     AQLLLYFFVLGLAGLKLAVEKRNAFLIFGLPLAIATMHLSWGAGFLWSFISGFASART
                     HG"
     gene            19109..20575
                     /locus_tag="PLE39_10370"
     CDS             19109..20575
                     /locus_tag="PLE39_10370"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNE68776.1"
                     /translation="MADIIKPPLRLRPSEQRTILLAGDLIASVGAMFAALFTWYQFSL
                     VREVARLVERGFSEARAERLAASIIELKIPVWFYILPLVWLLLMVDSYEPHTSASWRK
                     TLRGIAVAPIVGLLGYSLLFTFNQDPESLPRIGVGAFLIFASILTLAWRAIYIRLYTS
                     SGLMRRVLIVGAGKAGHSLADIYRKFSPPPFLLIGYIDDDFRKQSKSYHGFAVLGTSQ
                     KLLEIIETHRISDIVVAINGEIKGETFQTILDAQEQGVEVTRMPIMYEEMTQRVPVEH
                     LESDWVIRSFVDQVRVSGVYELFKRLMDIVGGLLGLLIFAVIFPFTALAIVLESGFPI
                     FYSQERLGKGGRVFMIYKFRSMVQNSEEDGEARPAIVNDPRVTRVGNFLRRTRLDEMP
                     QFLSVLTGEMSLVGPRAERPELVAQFQKQIPFYRARLLVKPGLTGWAQINYGYVATVK
                     ETVVKLEYDLYYIKHRTLNMDFNIVLRTIGTVLRRTGR"
     gene            20579..21505
                     /locus_tag="PLE39_10375"
     CDS             20579..21505
                     /locus_tag="PLE39_10375"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE68777.1"
                     /translation="MNYLVTGGAGFIGSHIVQALLKQGDKVRVFDNFSSGKRENLKGL
                     DVEVIEGDLRDASRVAEAVRGMDIVFHEAAFVSVPESMEKPQECFDVNVTGTSILFEA
                     ARKAGVRRAVIASSAAVYGDSTAMPLVEDTPLRQLSPYATSKRVDEKYAQLFTEFFGF
                     DVVALRYFNVYGPRQRPDSMYAAAVPIFIRRILDNKPITIFGDGGQSRDLVNVRDVVQ
                     ANLLASQHPAAPGQIFNVCTGVETRLLDLLDILYELFPNAPRHVHAEPRAGDIYRSIG
                     TPKKIMDTLGFKPQVTLADGLHEAVEEMRGQK"
     gene            21588..23471
                     /locus_tag="PLE39_10380"
     CDS             21588..23471
                     /locus_tag="PLE39_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNE68778.1"
                     /translation="MSSRPHIRNRFVLIGDLALIVISVLGSFALRLDVSELPFYFPAA
                     LVMCAVALLVKVPVYFYFGLYRRLWVYASTSELRLITIAVTSASALTSGVMILLSSIG
                     FIQPGMPRSALGIDWLFSLVLIGGSRFALRILAEQSSAPRDALRVGKRALIIGAGDAG
                     ALVVRELQKSSQLNLFPVGFLDDDPAKQNHQIYGVSVIGRVGKLADVLESQRIDEVII
                     AIPSAPGKIIRAVNDACRKKGIPSRTMPGIYELLGGKVSVNRLREVDITDLLRRDHVR
                     VNDEQVGQALEGKRVLVTGAGGSIGRELCRQIARRNPSQLILLGHGENSIFEILLELQ
                     NDYAALKLIPVIADIRNGQRLDSVFAQHHPQVVFHAAAHKHVPLMEANPVEAITNNVL
                     GTRNVVQAALDHNVERFILISTDKAVNPSSIYGATKRLAEAIVLDAAHKNKRAFAVVR
                     FGNVLGSRGSIIPIFKNQIANGGPVTITHPDMFRFFMTIPEAVYLVLQASSMNNGGEV
                     FVLNMGEPVRILDLAEDLIKLSGLEPQKDIEIAFTGIRPGEKLTEELWVEGTSLIQTP
                     HPDIFRLDQDAASALNLDLVQAIEQLSTLSLTTDAQSIARLLDQLIPGSTIFNAPHDY
                     LEV"
     gene            23473..>24357
                     /locus_tag="PLE39_10385"
     CDS             23473..>24357
                     /locus_tag="PLE39_10385"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNE68779.1"
                     /translation="MPEVSLSDWNQFVASHPDAHLLQMGQWGELKNDFGWKPVRLMLD
                     RETGAQILFRRLPLGLTLGYMPKPVDSGRLMVDSESFWREVDSICKKNHAIFLKIEPD
                     SWEETFQPSNLPTFKRSNHNIQPPRTVTLSIKEDEEEILARMKPKCRYNIRLAEKKGI
                     TVRAWSDISAFHEMMTVTGGRDNFGVHSKEYYQRAYELFHPIGTCELLVAEFEGRPLA
                     SLMVFSNAKRAWYVYGASNDQERNRMPTYLLQWEAIRWAKARGCEEYDLWGVPDEQEE
                     TLEANFETRRDGLWGVYRF"
BASE COUNT         5492 a         6079 c         6447 g         6339 t
ORIGIN      
        1 ccgtgaagaa atcggtgtat cgtttgcttg agaggctgaa aggacagatg gagtagatat
       61 gagcgacttt aatccctctc ctgaatttga agaaaagatt cgcaaagcga cggatgtgcc
      121 gggtgcgcgc cctgaatttg tcaataagtt gcgtaatgaa ctggcgagga ggccagtgaa
      181 aatgaaaccc cgttttatat tgaaacctgc atgggcgttt gcctttgtga tcgtggcgct
      241 tgcattgatt gccagcgcgc ctgccgcagt gaatgctttg aaaaaattgt ttgggtatgt
      301 gcccggtgtt gggttggtgg ataacagcag cggaatacgg gttttagcgg aacctgcttc
      361 agtaacgcga gagggtgtga cgctgaccgt gacacaggta ttggtttatc ctgaccacgt
      421 gcagttgatc tatgaagtca gcggcatcgc gacggagaat gatggtaact tcgcattaga
      481 tgcgacccaa aactttaccg cgttttgtgg ttcgccagat ggtgacgcca aattgcgcct
      541 gcctgatggc acgatcatcg agcgtgcttt tgacacagaa aaatatcctg aaaatatttt
      601 tgcgaccaaa cccgcgtatg aaacagtcat cccagcggat atgatggaaa tgacgatggt
      661 actcaagtgc cttccgtggg cgcgacttgg tgcggttcct gaaaattggg aagtgccgtt
      721 gaaattgaaa tacgttcccg ctggtgagac aatcggcgag cctgtgttgg atgtgacgcc
      781 ttccgcttcg agcgcagcct ctgatcaggg attgagtgtc actttggata aggttgtgcc
      841 acaggatgat ctttatagtt tttatctcac cattgcgccc gaacaggcag acgaatctgt
      901 gctggcatat ttccccactt acgccacact ggtggatgcg accggacaga agatctcttt
      961 ggtctatatg catcctttct ctggctcgca tgatgtggct gaaccgtggg aactccagtc
     1021 aattaccagg cccgcctacg gcccctacac tctggtactc gattctgttg cgacctacta
     1081 caatgaagat ggaagtcatt tcttcgatgt tgatttggga ccgaatccgc aagtggggca
     1141 gacttgggag atcaaccaaa ccttgaactt ggctggcacg gatgtgcgcg tcctttcggc
     1201 gacgatggcg gagagagacc tgtccatgtg gggcggggct gcaaacacgc agggacttgt
     1261 gttcaccttc gaagctgtgg atgggaagac gccattctcg ctggatatca tggatcgtga
     1321 tgttgaccat attatgaacg gctcgatctt cccgttcagt gatggtctga ctaatacttc
     1381 agaacaatat gtgaccgggc ttttctatga aaagggaatt ccgtctggaa aattatccat
     1441 cgctgtcaac gggcagacaa ccatgacaaa cggtcactgg gaatttcagt gggcgtcgcc
     1501 ggatcagacg ggagaggagt tgctcagcgc ttcggaatca atctcctctg cggctggttc
     1561 ctccggtgtg acaaccgaat tacgccgcgt ggtcaaaacc aatgatgggt atctattctt
     1621 cgttcacatg acaacacctg agcaaaaccc ggatttcaga gtcgttgagc cggttgaagt
     1681 ctccgtggtc gactcgaccg ggaaaaagat aaaactcaat ttggacggtc cgcaggttta
     1741 cagcgcaagg gatgaaaatc tatggcagtt cagcacagcg gagcaggttg ccgccggtcc
     1801 gctcaagctc gttgtggaca aggcgaaggt ttactattcc aaattcaatt ttgcgacccc
     1861 tccgcctgct gatgtatatc aaaatctcgt caaggaaaac tcctttattt tcgatgcagg
     1921 cgccgatccg caagtgggtc aagagtggat gttggatcaa gagtttgaag ctggcggata
     1981 tcacgggatc gtgaaatctg tccgtgctgt gacggttgac tcgcaaatgc tgcctttccc
     2041 cgaacttcaa cccgatgcca gcctcaacag agggtatgag ttcacggtcg aagcgatgga
     2101 tccgtccatc cagtggaatg tgagtttgtt cgtttccaaa cccgaaggcg atcccggatt
     2161 tgccgactgc ataggaggca tggatggaga agcaggttct gtaaccacac ataccatgag
     2221 ttgcagagga ctttccagcg gaattttaca ggttgaaatt tctgagatct cgatctggct
     2281 gaatgaaatt tgggaagtgg cttgggtgat gcccggtcag taggccaacg gggaacaatt
     2341 ttttcgtaaa catcgttgta tcaagagttc aatcttgata caacgatgtc ggttgaagga
     2401 aaaataatga ataaatatat aaagactttg cttgtggtaa tgatttttgg actttctgcc
     2461 tgtggacaat cacgcaccac gccgcaacag gatgtttttc tcgacccacc tgccattgta
     2521 gtgaaagctt cacgggaaaa ctgtccttcc atagtagctg aggttgggat gaccatagcc
     2581 tgggaaaatg tcgatacggt atctctgccg ataaagatcg aattcctgga tgaaaatggc
     2641 aaggtcactg atacgggact atctgtgatt gaccccgaga ctgttttctc aacaagcttc
     2701 cctgaggctg gggtctatta tgtctactgt actgaaaacg ccgatatacc ttcaacgatc
     2761 acagtgaaat aaaaaagagg ctgggtagtt tcaaccaccc agcctcttca atacaaaaat
     2821 cacatcacat cgggcgcttg aatatcaagc aggcgtaacg cattggcaat ggtttgcttg
     2881 gccgcagcga ccagtcgcag acgggcattt ttggtgtttt cgtcctcggt ctgcatgaca
     2941 ttgaccgcat gatagaacga gtggaaggtg ttcgccagtt cataagcgta cgacgccatc
     3001 accagcggac gatattcttc cgcggcctgt tgcacagcct ttgggaagcg ggaaatctgt
     3061 tcgatcaatt cgatctcatg tttagtaaag tcgtagttga acgtttgaac gttcgaacgt
     3121 tcaaacgttg aaacgcctgc cttcttaaga attgaatttg cccgcacatg tgcattttga
     3181 atatacggac ctgtgcggcc atcgaaggat agagcttcgt tgatgtcaaa aacgatgtct
     3241 ttgttgttgt ccacagaaag catggaatag accagcgcgc ccatgccgat ctggtaggcg
     3301 accttttcac gttcttcggc ggggatatcg gcgctttttt cggtttccac ggaaaggaca
     3361 cgcttcactg cttcatccgc tacatctttg aagagcacca cacgtccacg ccgtgccgac
     3421 atggcgcctt cgggcaggct cacaaatccg tagccgaggt ggtagcactt ttctgattgc
     3481 gggaagcccc acaacttgag aatggcaaaa gcctgttgta aatgcagtga ctgacgcaca
     3541 tccaccacgt agatcgagcg gtcaacgtga tattgttcaa acttcacttt ggcaagcgca
     3601 aggtctttgg tgagatacaa ggtggtgcca tcgcgccgca gaatgacatt ggtgcgatac
     3661 ttttccttgg ttagtcccag tttctcatcg atctttacaa tcaccgcgcc gccctgtgga
     3721 cgttcgtcat cggcgatatt ctttgcgatc aattcttcaa cgatggcttt ggaaggttca
     3781 tccacttctg attcgtagaa ccacacatcc atttttacat cgagcatccg cagtacatcg
     3841 cgcaattctt cgaggctcca ttcgcgggtc tcgtgccaca actttcgtac ggcttcatcg
     3901 cctgcatccc atttgcgata catctcgcgg cgttcagctt cataggcttc gcgctgggca
     3961 gtttgctctg gcgtttcgtt ttccttcttt tcaagcaagg ccgtggcttc cacgtacaac
     4021 ttcgccaacc attgtccgcg ctcgtgaatg cctgtgggtt cctgtccctt gtaaaacttt
     4081 tcgtagatcc acatcactgt gatcacaccg agtccaaggt caccgggata tgaagcgcgg
     4141 atcgtatcga agcctgcgaa ttccaccagc cttgccagca cttcacccag gatcgtatta
     4201 cgcgcatgcc caatatgaaa ggagtggtgt gtgttcggct gggcatattc aaccatgact
     4261 ctttcatttt tcgccgcacc ccgcccaaag ttcaggccag aggcgagcac ttcatccaca
     4321 acgcgttggg cgtaatcgga tgtcttgaaa tagacgttga gatatccctt caccgcttcg
     4381 atgtgactga tcccatccac gcttccgatc tgacctcgaa cctgctcggc gatttcctgg
     4441 gccttttgcg gcaccggaag tttgttgccc ttgccgagtt tcacttcatt ggcggcggtc
     4501 tgaaaaaacg acgtggcgaa accccattcg cccgaaaagg ggatgggctg ccatttgagt
     4561 tctgccagtg caatgtcgtt ggcctggcag taggctttga tcttttcttc gatgagttgt
     4621 tgttcttttg taaacattgg tttgattata gacgccatcc ccaaatttag gggagaaatt
     4681 gagataacaa aaacgggagc gccgttttga gggcgttccc gttttgtggt gttgaaggaa
     4741 agactaggct ttcttttcgg acttcgcaga aaggaaagcg gccaatttct tcatcaagcg
     4801 gcctttgcgg cgggaggcat tgttcgggtg aataacgcct ttctgggctg ctttgtccag
     4861 cgcgctgacc gccagcatga tatcttcctg tgtttcggga gcgctctttg cgagcgagct
     4921 gcgggcggct gtgatggcag tacgggccgc accgcggaag ttgcggttgt ggatgcgacg
     4981 cttttcattt tgacgattgc gtttgatttg tgaattaatg ttagccaagg ttattctcca
     5041 gatgttacaa ctttcatttt atgacagcgc cgtattttac atgagggaaa tacatttgtc
     5101 cagtaatttt gcgggcaaat tacggggtgg gagtcgctgt ggccgtggca ggcagggcgt
     5161 tgatcggtgt gaatgtgacc gtgggtaaca atgtgcctgg gcccggtgta atgggggtgc
     5221 tggtgggcgg gcgtgtggcc gtagctgtga cgacattcac aggaatttgc agcgtatcac
     5281 cgactttgat cgcattcggg tctgtgatgc cattggccgc gatgatctca tcaatggtgc
     5341 tgttaaaggc ggcggcaata ctgcccagtg agtcaccgct ttggatgatg tattcgatct
     5401 tggtccccgg ggccaggttg gctgggagcg gggtggcggt gggcaatggc atgcccggat
     5461 agggcaacac aatggattgt cccggcagaa tgttcagcgt tgcagggtcc acgccgatgg
     5521 ggaagcctga gtcggcgatg ccgccatagg ggttgaggag caagatcaag gcaacgccat
     5581 tttcaccaag gttgaatttt tcaacaattg agaaaagcga gtcgcccgct tccacagtgt
     5641 agttgaagat cgtggagaac gtcggtgtgg gggtgatggt gggtgtttct gttgcggtcg
     5701 ccgtcgatgt cggggtggat gtgctggttg gcgtgaacgt catggttggg gtgggggttt
     5761 cagtagccaa aagcgcgtta agtggactgc tgtcaccggt cagccagaca accagcatga
     5821 tgatgccgcc taacaccagt acgcctgcgg cgattaggac aatgttaggg ttgcgcttgc
     5881 gcttgcgcag caggctgatg gtgcccgggt tgggtgaaga agaaatcgga ggtctgttat
     5941 tcatgaattg ttccttttga taaagtgcct tcttgttggt cggtcccagc ggcacgatat
     6001 aaaataattc tacctgaaaa cgagaatcgc gtaaggaggc tcaaacaaag atagcggacg
     6061 aaccaataaa aaacagaaca ggtaaacaat ttgtttacct gttctgtggg tttctctgcc
     6121 tactttacat gggctttcaa atattttccc gtgtaggatt cttttacctt catcacctgt
     6181 tcaggggtgc cttcggctaa caactggccg ccacggtcac cgccctccgg gccgaggtct
     6241 atcagccagt cagcgatctt gataatgtcg aggttgtgct caatgatgac gaccgaattg
     6301 cccgaatcca ccaaccgttg caacacttcg atcaatttgt ggacgtcggc agtgtgcagg
     6361 cctaccgagg gctcatccaa cacatacaag gttcgcccgg tggctcggcg tgagagttct
     6421 tttgagagtt tcacacgctg ggcctcacca cctgaaagcg tggtggcggg ctgcccgacc
     6481 cgcacatatc ccagccccac ttcttgcagc agtttcaact tgctctgcat gggtggataa
     6541 tttttgaact catcaagcgc gtggtcaacg gtcatatcca gaatgtcgga aatgctgtat
     6601 ttatcgtgga agagcacctg caaggtttcg cggttgaaac gcttgccgtg gcaaacgtcg
     6661 catggcacat acacatcggg tagaaactgc atttcgatgc gcaattcacc ctgcccctga
     6721 caggcttcgc aacgcccgcc atggacatta aaggagaagc gtccgggctt gtatccacgc
     6781 accttgcttt cgggtaattc tgcaaacaac ttgcggatct catcaaaaag accggtgtag
     6841 gtacctgggt tggaacgcgg tgtacgtccg atcggtgact ggtcgatatt gatgatcttg
     6901 tcgaggtgtt cgatcccttc gatccgttcg tgctctcctg cgctcgtccg tgccgacatc
     6961 aattttgcgg ccagcgagtt atacagaatg tcgctcatca aagtggattt gcccgaccct
     7021 gaaactccgg tgatgcaaac catctttcca agaggaatgg agacattgat gcccttcaaa
     7081 ttgtttgccg ttgcattgac aattttcaac gccttgccat ttccttcgcg cctctttttc
     7141 gggacttcga tctgttttcg ccccgagagg taggctcccg tcaacgactt tgggtgcgcc
     7201 aaaatctgtt tcggtgtacc ttcagcgatg acttcaccgc catgctcacc tgcggcaggg
     7261 ccgaggtcaa tgatccaatc ggattcgcgg atggtctcat catcgtgttc aactaccaaa
     7321 acggtattgc ccagatcgcg cagacctttc agcgtttcaa gcaatcgtga attgtcgcga
     7381 gggtgcaggc caatggaagg ctcatccaaa acgtacaaaa caccgaccaa ccttgacccc
     7441 acctgtgttg ccagacggat gcgttgtgct tcgccgcctg aaagcgtggc agcggagcga
     7501 ttcagggtca aataatccaa cccaacgttg acgaggaagt taaggcgttc gtgtatttcc
     7561 ttgataaccc tctcggcaat cgtcttttgc tttgaggtca acggcgaacc tttgccggag
     7621 atctttttga cccaatcaag ggtcttcaaa acaggccagg aatttgcttc cacaatattc
     7681 acactgtcca ctgtaacagc cagcgcggcg gggtttagcc ttttgccgtt gcaggtggga
     7741 cagggacggt ctgacatgaa ttcgctgatt ttttcgcgta tccactcgga attcgtttcg
     7801 cggtagcggc gttcgaggtt ggtaatcaca ccttcgaaag cgcgggtgaa tttgaattca
     7861 tttccatttg agtttttgta gttcatttgg atctgttttt cgcccgtgcc gtacaggatg
     7921 attttcatct gatccttggt gagttcacgt acgggtttgt tgaggtcgat cttgtaggcg
     7981 tgagcggcgg cttcaaggcc ctgccaatag tagccgcctt cttctttcgg tccactccac
     8041 tccatgctgg agatcgctcc ctcgttcaga gaccggtcag ggtccggtat gatgcgttcg
     8101 ggatctattt ccagttttga gccaagcccc tgacagtccg ggcaggcacc ctgcggcgtg
     8161 ttaaaggaga acgtccgcgg ctcgatctct gaaatactga caccgtgttc ggggcaggcg
     8221 aggtgttcgg agaattggat gtcttctttt ttatccacca ggttgacggt aatgtagcct
     8281 tcaccaaact tcaaagcggt ttcgactgag tcggtcaggc gggtgcgagc ggattttttt
     8341 tcctctttat cgccttcctg tgaaatgaca aggcgatcaa caacggcttc gatcgtgtgc
     8401 tgtttgtagc ggtcaagttc gatctcttca tcgagggaat gggtgttgcc atccacacgg
     8461 gcacgcgtaa aacctgcttt gcgaatctct tcgaagacgg cttgataggt ccctttgcgg
     8521 gcgcggacaa gcggagcgag gatcaaaacg cgtgaacctt caggcagttt ttcgatcttg
     8581 tccacgatct cttgtgccga ttgtttgacg acctctcgtc cgcagatggg gcagtgtggg
     8641 atgccggcac gcgcaaacaa caaacgcatg taatcgtaga tctcggtcac ggtgccgacc
     8701 gtggagcggg gattgtggga tgtggatttc tggtcaatgg atacggcagg ggaaagccca
     8761 tcaatgtaat caacatcggg tttatccatc tgcccgatga actggcgcgc atacgcagag
     8821 agcgactcaa catagcggcg ctgcccctct gcaaagatag tgtcaaaggc cagtgatgac
     8881 ttgcccgacc ccgacaagcc tgtaatgacg acgaacttgt cacgtggaat ttccacggtg
     8941 atatttttca ggttatggac tcgtgcgccc acgacgcgaa tgacattggt tgacatggtt
     9001 ctccttggat ggggagcaat tgtagcacaa atgttttatt ctttgcatgt ttttttcatt
     9061 gcccatccct tgccgttcgg ccttgtattt cgcgttatac tcagtgcaac ttctcaaatc
     9121 cggcggtgtt attttagtaa acccgccggt aaggttttaa tcttgctgga tatggaatac
     9181 aacgaagaag agattctcct gcgggcctcc cagggtgacc gtgattcttt tggtcaactg
     9241 tatgcccatt atgtagagcg catcttcaat tatgtctatt atcggaccgg gaatactcac
     9301 gatgccgaag atttaactgc ccgcgtcttt caacgggcga tgaagcatat cgtgaattac
     9361 acggaccggg gcgtgccatt ctcagcctgg ttgtatcgga tcgctcacaa cctggtggca
     9421 aactggcatc gtgatcgcag ccgcaagcag gagattccgc ttgatgacct ccccattctg
     9481 ccaaccaagg gcgatcatcc agaaagaaac ctcgttcgtt cacaagagca ggatgtgttg
     9541 ttgaaatgat tcgaaccctg cctgcagaac gacaaaattt actgatcttg aagtttgtcg
     9601 agaatatgtc caatgccgag atcggcgcaa tcatgggtag aagcgaggga gctgtgaaga
     9661 gtttgtatca ccgtacactt ctggctttgc gtgaccaact ggaagaccaa aaccttaata
     9721 tcgaaggaga gtagaccatg ttgagaattg tgaccgacgg cgcagcagat atcctccctg
     9781 catgggggca ggagtatggg attgaagtga tcccagtaaa tatcctgttt ggggaaaaat
     9841 cgtatctcca gggtgtcgaa ttggacaatg agggttttta taaactggtg gatgacacca
     9901 aacgcattcc gaagacctca caaccctccc cgcaccagtt tgttgagttt taccgcaaga
     9961 tcgcccagaa aggcgatacg attctttcaa ttcatatcac cgcaaaattg tcgggtactt
    10021 atgcatcggc gatcgccgcg gcggaagaat tgaaggatga attcaaggtt atccccatcg
    10081 attcagcggt tggttcattg ggcatcggta tgatgtgccg tgaagcccgc aggcttgagc
    10141 gcgcaggaaa aaccgtggat gagatcgtaa gttaccttga agacatcaaa tataagatcc
    10201 gtgtcattct gacccttgat actttggaat atgccaagat gtcgggtcgt gtgaaaactt
    10261 tgcaggcggc attggcctct ttgcttaacg tgaagccaat cgcaattctc aggaatggcg
    10321 atttgaatat ggcggaacgt gtccgtaccc gcaaggcatc tctcgatcgt gtgattgaaa
    10381 ttgctgaaga agagttcggc aagaaacctg tctatctggc agtggtgcat gctcgtgacg
    10441 ccaaatcggg tcagggtttg ctcgatgacg cgaaatctca tttcaaccat aaagaaacca
    10501 tgctcagcga tttatccatc tccattgctg caaatcttgg gcctggcacg gtggggttga
    10561 tcctttaccc cgcagaataa acaggtgatt tacgaatgat gactagaaga aacacatcca
    10621 ccacactgga agatttggat acccttgaag cgcatctggc tgggaccttg aagccgatct
    10681 cgcctccgaa ggaacttgtc cagcgccttg gagcacggat ccgtgtgccc agccgcagtg
    10741 agatcacttt gcgcttcaca gattggcggc ggatgttctt tgtagttggc ggtgtggttt
    10801 cggggatgct tctgctcatc actgtcgcac gcgccttgta ttacctggct gggcgcagga
    10861 tgtaagctgg tcatctgaaa gagaccgacc gctttgcctg aaaaaataga gaatatgaaa
    10921 aaatcaccca ccaaggctct gccttttgtg ggtgattttg ttttcgtaaa aagattggga
    10981 gatggcgatt aattgctcaa caaccaattg cacagttttc tggcttctgc aatgaggtcg
    11041 gcatcaacag ggagggtaaa ttcttcagtg acgcgcactg tcaggttgtc aacggccagt
    11101 ttcaggtcag gaggaagagc cgcgttgtct ttaagaagat tcagcagatc gtacgaactc
    11161 atgcttggga ttttgtcccc tttgcgggtg aggtaatccc gtatggccac cccggcggca
    11221 cggcgggcac aaacgcgggc ctgcccttcg tttttggcga tgcgggcttg ttgggctttt
    11281 tcaaattctt tttgtaattg tgtggttaca ttatccatgc cgaaaattat aatctaccgc
    11341 ttaaccatgg ggctataatt atgggaaccg aactcaaagg agaaaacatg aagcccacgt
    11401 tgtttcgaat ttttggatat ttaattatag tgagcctcgc gcttgcagca tgtacaccca
    11461 ctgcggtgcc aactgaagcg gtggaggaag gggattttct gggggcaatg tctcaaccct
    11521 acgaactgac aggttcttat accgttacca atgattttgt gcttgcaacc tattatgtgg
    11581 aaaatgctgt tgctttgacc gatatgcacg gatttgtatt acgtgacctc gaatgggaga
    11641 ttcccgttga ttcgcaggtc cttgggttta tgacctttga tgcagatgcc ctcggcggca
    11701 cgtatgaact gagcctgcct gccataccgc aaggcgaatt caacgatgtg gataacaacg
    11761 gcgccgctga tgcgggtgta cagatctttg cagtggcata ttcacccaat gtgtacggcg
    11821 gccctttcgc tgaaggtgat gaccgctcgc gcggctggcc ttcctacctg gcttcggtaa
    11881 agaccgactc ggaaaacaag gatgaagtca ttggcgggaa actggtggtg tgggccgaag
    11941 atgccaacca gcaattcccc acaagttttg gagatgatgg tttgctcttc actgccgatg
    12001 atcccgtcgc tcccatttcg cagggatatt caatcgttga cctggatcaa gttccttttg
    12061 cggttactca agatgccatc tcagacctgc ctttgtacga acctgaagac atcgccacaa
    12121 aggattactc taccctgtct tacaccgaag cctttgacaa aatgttcgaa gtgattcgca
    12181 atgaatatgc ctttgctgat attcccggcg aagcccccga ttgggatgcc ttgtacgctg
    12241 aattgaaacc gcgcgtggaa caggccgaaa agaccagcga cccgaatgct ttttaccttg
    12301 ccctgcgcga tctgacttgg gctttcgatg acggccatgt cagcctcaac ggcgggcagt
    12361 atttccagca ggatttcgcc acagccgttt caggcggata cggttttgcg attcgtgaat
    12421 tggatgatgg ccgtgtgatc gtgatcttcg taatggacgg cggccccgcg caggcggctg
    12481 gtattcaggt cggtgcagaa gtgaccgaat tcaatggcct gcccatttcc gatgccatct
    12541 ccggggcgca atcgtattcc aatcaatcct cagagtttgc gatccgttac cagaaatctc
    12601 gttatctgtt gcgtgccttg cctggcacag aagccacggt gaaatttgcc aatgcagatg
    12661 gcattccgca ggtggttacc ctgaccgcca ttgaggaacg tcagagcttc agccgcactt
    12721 cactgtactt cggtgtggat aacagttcac tcctgccagt ggatttgaag atcattcctg
    12781 tcaacaacga taatgttggc tacattcgca tcaacagcaa ttacgatgac ctcaatctcg
    12841 tcatccgcct ttttgaacgt gcgctcaaac aattcgaagc gaatgaagtg gcgggcatca
    12901 tcattgatat gcgttacaac aatggcggtg cgccgctcgg gctggctggt ttcttcaccg
    12961 agcaggaaat tccactcgga caactcgaat actacagtga taaaacgggc aagtttgaac
    13021 ccgaaggcct gcgccagaaa ttcctgccca atcaaaacca atatcgcttt gaaaagaccg
    13081 tcctgctggt tggccctgct tgttttagtg cctgcgagat cgaagcctac gggttcagcc
    13141 aggtaccagg gatggttgtt gtcggcctca cgccaaccag cggcgttgaa gcagagaccg
    13201 cacgcggcac gttcaatcta ccagaaggat tcagcgtgac ggttcccaca ggacgcttta
    13261 ccctgcccga tggcagcatc ttcctcgagg ggcagggagt gccgcccacg ctgcgaatcc
    13321 ccatcaacga ggaaactgct acggctatcg aagacattgt tctacaagca ggcattgatg
    13381 cagtgctcca gccgtttggc gctggcgtaa cccccagtgc agccccaaag gttgcctcac
    13441 aaaccgaagc ggaatctgcg ctttcttcgg gtgctccatt tttggaagac cttgcccgcg
    13501 aaagctatga tcccgccatc acctttgccc agcctggaac tgcaacctat tccatttcca
    13561 tgaccgatcc cgaacctgtg atctggggct attcctggtg cgccgttgat gccgccacag
    13621 tcgcatccaa cttcgaaaat attgatatgc agttcatgct tgatggaaag gaagtgccta
    13681 ctgattcatt tggcactctc gaactggaaa caggtggaaa agtctgccgc ctcatctaca
    13741 cctccctcag tgagtggact tcgggcgagc atcacctctc cacaaccgcc acattcaccg
    13801 ccgccattaa cgatggcagc gccaatttcg aagctggaga atatatcctc gaatatacgg
    13861 tgtatgtcaa gccataatct ggttgttggt gccatggcaa tgggacctgg catatttaag
    13921 cgaatggaag tttatgatca acattttcat gaaatcacca ttgccaaata ctttggcagt
    13981 tattgtgctg ttagccttga taattacctg ggttacgtca atcgtattaa gtatgcgaga
    14041 tgttcctgtt gaaacggaaa aaggctggta tgccaatctg ctccctatat tcagtattct
    14101 tggcatacca gccgtgctgg atttgctgca aacaaaagga gttacctttg tttttgcggc
    14161 gatcgtcttt acggttttac ttcttaacat tgttgtccca attctaaaga tttatggaaa
    14221 atcttcgcac tttctggtcg cagattggta caagtggtca gtcttaattt caaccgtcgg
    14281 tgggctggca gtggctggct atttgacatt tgttgaagca acaaacgcgc cagttgtgtg
    14341 tggcccatct gatgggtgtg ctgctgttca aaacagtaag tatgctgttc tttttggggt
    14401 tttgccagtt ggtgttttag gcctggctgg gtatgtcgga attcttgcag gctggcttgt
    14461 tttacacttc ggtcctgatg caataaaaag aatggcggca ttggctgttt gggcgatgtg
    14521 tatatttggt gtgttgtttg agatttactt gacgtttttg gagcctttcg ttattggctc
    14581 tacttgcatg tggtgtatct cctctgctgt gttgatgatt atcctcttgc tggtttcaac
    14641 acccgctgct caacaggcat ttgttataaa tgacgattag agcttgggct ggcggctaat
    14701 atggcggatt actgactttg cagaagtttt ccctgaatct ttttcgctgc aaagccataa
    14761 tttacaatct ttttacaatc cagccatatc caccttacct gccgcatcta aaatttcatc
    14821 gtaacctgaa aggaggttta cgatgaaaac catgttcaag aaaacaatat tggtggcaat
    14881 tgccgccgcc ctcgttttgg ctgcgcttcc attttccagc gcatttgcaa tgaatggcag
    14941 tgctgaaggc gaggtgacca acgaaaagct ggagcagatg tgggcacgtc aactgcaggc
    15001 gtatgagcgg gtcggcaaag gcttcgaaaa gaccgatgag ttcactgcca aagtgcagga
    15061 tcgcattgac aaagccgctg ccaacggcaa ggatatcacc gccttgcagg ccgcgttgga
    15121 tgcctacgaa gccgcggtga agaatgctcg ccctgcttat gaaagcattc agggcattgt
    15181 cagtgcgcat cagggttttg atgcaaacgg aaaagtgacc gatgccgaaa aggccaaagc
    15241 cactgtgcaa gcgatgcgta ccaaaatgca ggaggtaaaa acctccatga atggcacgtt
    15301 caaggccctg cgtgatgcga tcaaggcttt ccgtgaagcc aaccagcccg ctgagggaac
    15361 ttctgaacgc ggtacgtagt gtgatgttga caggaccaac gcaaaagatc gcgctggtcc
    15421 tgtttttgtc ttctttgaaa atattttgca gcagatggat tcgctcattt gtgtttggtt
    15481 ttcatgtcat tttcattaaa gggcctgcaa agccaaatct gtattaaaat caacgcgtgc
    15541 gaatactcgt tattatttgc aaaattttct caaagaggta cttatgaaaa agaaagtaat
    15601 tcaaactgaa aaagccccca aagcgattgg tccatattca caagccattc gcacagatgc
    15661 cttcatcttc accgctgggc agatcggcat tgacccgaca acgggtgagt tgatctcagg
    15721 tgcagtggaa gaacagacac gccaggcgtt gagtaattta cgaaacgtga tcgaggctgc
    15781 tggctccaac atggacaacg tagtcaagac caccgttttt ctaaaagaca tggccgattt
    15841 tccaaagatg aatgcaattt atgccgaatt cttcggcgag aatccgcctg cccgcagcac
    15901 catcgctgtg gcggccctgc ccaagggcgg cctggttgaa gttgaagcgg ttgtgctggc
    15961 tgtctaatta atcatcaggc tcattatggc ttccgaactc atcctccttg ttgacgacga
    16021 accttccatc atccagcttt cgcgcatgta ttttgagcgc gatggatttc gtattcagga
    16081 ggttggagat ggcgaagccg cactcgaagc cgttacaaaa catcaccctg ctttgatcgt
    16141 gttggatgtg atgctcccaa aattggatgg ctttgaggtc tgccgtaccc tgcgcgccaa
    16201 aggagaccag acgcccatca tcatgctcac cgctcgcgat gaagacattg ataagatcct
    16261 cggccttgag ctcggcgcag atgattacct gaccaagcct ttcaatccgc gtgaattgat
    16321 cgcgcgtgtc aaggcgattt tacgcaggag tgacggcaaa aaacaagcgg atggaaaacc
    16381 gcttcatcgc ggcgacctga ccattgatcc tgtttctcgt gaagtttcca tcgcctcgca
    16441 aatcctcgat ctgcgcacgc aggagtttga cctgctactt acgttggcag aacagcctgg
    16501 gcgtgtcttc acccgtgagc aattattgca actggcctgg ggatttgatt tctacggcca
    16561 gacccgcaca gtggatgtac acattgccca tttgcgcaaa aagcttgaag gctcaaacat
    16621 aaaaatcgaa accgtcacag gtgtcggtta caaacttgtt gtataacctt caactttcaa
    16681 cctgtaacct tcaaccctaa tgttttcctc cctgcgttcc cgcctttggt tgagttatgc
    16741 acttcttatc gtcacggcat tgggtgtcgt ggcgataatt ctgtttacct acctgctcac
    16801 caaccccttg ttgtatcgtc aaacccttga aagactgcgg gctgtgcaag gtgtggtact
    16861 ggacaattcc aggctgccca acgcccagcc gatctccatt gcagcgcaga aggcttctct
    16921 gacgttcgat gtacggattg ttcagtactc acgggaaaag caagtgctat cagataccta
    16981 ctcaagcaaa gagcctgttt tgccgttccc tgaaaaacga gtctttgcgc ggaatgtccc
    17041 attggtccgt gatgagaacg ggcaggcatg gttgtattcg gttgagatct tgccagataa
    17101 aagctacttg ttggtggctg ccccgcgacc gaaactctcc atttcgaatg ttttcacaga
    17161 tgattttctg ccgctcattt tcaggagcgg attgcttgcc ttactgcttt ctctggtggt
    17221 cgcctttttc ttcgcgcgtt ggatcgccga cccgctgcaa aaagtggtgg cggccgcgcg
    17281 tgccatgccg tcagcggaga taaagcctgt tgaaatcggc ggcccgcacg aggtccagga
    17341 attgacgcgt gcttttaatt ccatggtcgc gcggactcag gcaagtcaac ggtcacagcg
    17401 agattttgtt gccaatgtct ctcatgaatt gaagacgccg cttacttcca ttcagggatt
    17461 tgcgcaggcg atgcttgacg gtacggcaga ttcggaagag gcgcgtcaga aggctgctca
    17521 agtaatctac gacgaatcag ggcggatgca ccgcatggtg ctggatctct tggatctggc
    17581 acggctcgat tcaggcacgg cagatattac gatgtctcct gtgaagatgt ctgctttgtt
    17641 gaatgcagtt gctgagaaat tcgcgccgca atccacacgt acgggggtgc gtattcaagt
    17701 ggaagccgcc gcagacctgc catccatcac tgcagacggt gacaggttgg cgcaggtttt
    17761 taccaacctg gtggataatg ccctcaaatt tacgccgtcg ggaggttcca ttttgttgca
    17821 ggcttccatg cttgatggcg aaatgcgtgt tgcggttact gatacggggg ctggcattcc
    17881 tgccgaagcg cagaagcata tctttgaccg tttttatcag gccgaccccg cccgcaaggg
    17941 tggtgcaaag catggtgctg gtctggggct ggccattgcc agtgagattg tgcaagcgca
    18001 tggtggtaga ataagcgtcc gcagtaggca gggggagggt gccacctttg aggtgttcct
    18061 gccgctggtc agaaaataac caatttcggg aaattttcct ttaaaatgcc gccctttgtc
    18121 tcgatcattg tcccttgtta taacgaagaa gccaccattc gtcatttgtt gaatgccgtt
    18181 tttgcgcaga cgtacccgcg cgcacagatg gaattgatca tttcagacgg catgtccacc
    18241 gatcggacgc gggaggtggt ggaggcgttt caaaaggaac acgctgatct tgagattcgg
    18301 gtggtcaata acacggcaag ggccattcct tcagcactga atcaggccat tcgtgagggg
    18361 cgaggcgatg tttttgtccg cctggatgct cactcgatgc ccatccctga atatgtggag
    18421 cgttgtgtgg cggcacatga agcgggaaag ggcgaaaata tcggaggggt gtgggagatt
    18481 cggccaggtg ctgatacggc cattgccaaa gcgatctcgt ttgcggcggc gcatccgctg
    18541 ggcgtgggcg atgccatgta tcgactcaat gccagtgcgg gagcggtgga tacggtgccg
    18601 ttcggttcgt tccgacgtgc gctcatcgaa aagatcggcc tgttcgatga gactttgctg
    18661 gcaaatgaag attacgaatt caacacccgt gtgcgcgaat caggcgggac ggtctggctt
    18721 gacccctcga ttcgttcggt atatttttcg agaagcacac tgggcagcct gtcggcgcaa
    18781 tactggcgtt acgggttttg gaagttgaaa atgcttaagc gctatccaca cacattgcgc
    18841 tggcggcagg ctttgccgcc cgtatttgtg tcatccctga ttttttttat tgtgttatcc
    18901 ttgtttttcg ggcttgcaaa atatgccctg gcggcccagt tgctgcttta tttttttgtc
    18961 cttggcctgg ctggtttgaa actggcggtt gaaaaacgaa atgcttttct gattttcggt
    19021 ctgccgcttg ccattgcaac catgcacctt tcgtggggcg ctggtttcct gtggagtttt
    19081 atttcgggct ttgcctcggc acgtactcat ggctgatatt atcaaacccc cccttcgatt
    19141 aagacccagc gaacagcgca cgatcctgtt ggcgggtgac ctgatcgcat ctgtcggtgc
    19201 gatgtttgcg gctttgttca cctggtatca gttctcgctt gtgcgggaag tggcgcgttt
    19261 ggtcgagcgc ggattttccg aggcccgcgc cgagcgtttg gcagccagca tcattgaact
    19321 caagattccc gtctggttct atatccttcc actggtctgg ttgctgctga tggtggattc
    19381 gtacgagccg cacacctcag ccagttggag aaagaccctg cgtggaatcg cggtcgctcc
    19441 cattgtcggc ttgctgggat attccctgct ctttactttt aatcaagacc ctgaatcgct
    19501 cccacgtatc ggcgtgggtg cgtttttgat tttcgcatcg atcctcaccc tggcctggcg
    19561 cgccatctat attcgactct acacttcatc agggttgatg cgccgcgtac tgatcgtcgg
    19621 cgcagggaag gcggggcatt cgctggcaga tatctatcga aaattcagcc cgccgccctt
    19681 cctgctcatt ggctacattg acgatgattt ccgcaagcaa tccaagtcgt atcatggttt
    19741 tgctgtgttg ggcacgagcc agaaactgct tgagatcatc gaaacgcatc gcatttcgga
    19801 tattgtggtt gccatcaatg gtgaaatcaa aggggagacc tttcaaacca ttttggacgc
    19861 acaggaacaa ggcgtggaag tgacgcgcat gcccatcatg tacgaagaga tgacccagcg
    19921 tgtgcccgtc gaacaccttg aatcagattg ggtgattcgc tcgttcgtgg accaggtccg
    19981 cgtaagcggg gtgtatgaac tgttcaaacg gctgatggat attgtcggcg gcttgttggg
    20041 tttgctgatc ttcgcggtca tttttccgtt cactgcatta gccatcgtgc tggagagcgg
    20101 atttccgatc ttttattcac aggaacgact cggcaagggc ggccgtgtgt tcatgatcta
    20161 caaattccgt tcaatggtgc aaaactcgga agaagacggt gaagcccgcc cggcgattgt
    20221 caatgatccg cgtgtgaccc gtgttggaaa tttcttgcgc cgtacccgcc ttgatgaaat
    20281 gccccaattc ctgagtgtgc tgacagggga gatgagcctt gtggggccgc gtgccgaacg
    20341 ccccgagctt gtagcccagt tccaaaaaca gattcccttc taccgtgcgc gcctgctggt
    20401 gaaaccaggc ctgacgggtt gggcgcagat caattatggc tacgtggcta ccgtaaaaga
    20461 aaccgtggtc aagttagagt atgacctgta ttacatcaag catcgtaccc tcaacatgga
    20521 tttcaatatc gtgttgcgga ccatcggaac cgtattaaga agaacaggaa ggtaaacaat
    20581 gaattattta gtcactggcg gtgcagggtt tattggctct catatcgtgc aggctctttt
    20641 gaaacagggc gacaaggtgc gcgtgttcga taatttctct tcagggaaac gtgaaaactt
    20701 gaagggcctt gacgttgagg ttatcgaagg cgatctgcgt gatgcttcgc gtgtggcgga
    20761 agccgtacgt ggcatggata ttgttttcca tgaagcggct tttgtctctg tgcccgaatc
    20821 aatggagaag ccgcaggaat gtttcgatgt gaacgtgact ggtacatcca tcctgtttga
    20881 agccgcccgc aaggcaggtg ttcggcgtgc tgtgatcgca tccagtgcgg cggtttatgg
    20941 tgattccact gccatgccgc tcgtggaaga tactccgttg cgccagcttt caccttacgc
    21001 cacctccaaa cgtgtggacg aaaaatatgc ccagcttttc accgagttct tcggttttga
    21061 cgtggtggcc ctgcgctact tcaatgtcta cggtccgcgc caacgcccag actcgatgta
    21121 cgccgccgct gttccgatct ttatccgccg cattttggat aacaaaccca tcaccatttt
    21181 cggtgatggc ggccaaagcc gtgaccttgt caacgtgcgt gatgtggtgc aggccaacct
    21241 tctggcttcc cagcatcccg ctgcgcccgg gcagatcttc aacgtttgca ccggggtgga
    21301 aacccgcctc cttgacctgc tcgatattct ctacgaactc ttcccgaacg cgcccaggca
    21361 tgtccacgct gagccgcgtg ctggtgatat ctaccgctcc atcggcactc caaagaagat
    21421 catggacacc ctcggattca agccccaggt gactctggct gatggacttc atgaggctgt
    21481 ggaagaaatg cgcgggcaga aatagtaatt agttattagt gaatagtgat gttcgctgac
    21541 aactgatcgc ttttcatccc tcattcttcc tccttcacct ttagaaaatg tcttcccgtc
    21601 cgcatattcg caatcgcttc gtcttgatcg gcgaccttgc cctcatcgtg atttcggtgt
    21661 tgggcagttt tgcgttaagg ctcgatgtaa gcgagcttcc tttttatttc cctgcggcat
    21721 tggtgatgtg tgcggttgct ttgcttgtca aagtgcctgt gtatttttac tttggcctgt
    21781 accgccgttt atgggtgtat gccagtacaa gtgaattgcg gctcatcacc attgcagtga
    21841 cctctgcttc ggcgctcaca tcgggcgtca tgatcctgct cagcagtatc gggtttattc
    21901 agcctggcat gccgcggtct gcccttggca ttgattggct tttttcactt gtgcttattg
    21961 gaggctcaag gtttgccttg cgcattctgg ccgaacaatc gtcggcgccg cgtgatgcgc
    22021 tgcgggtggg caaacgtgcc ctcatcattg gcgcagggga tgcgggtgct ctggtcgtgc
    22081 gcgaattgca aaaatcatcc cagttgaatc tgttcccagt gggtttcctt gatgacgacc
    22141 ccgccaaaca aaatcaccag atttacggtg tttctgtgat cggcagggtt ggaaaacttg
    22201 ccgatgtcct tgaatctcag cgcattgacg aggtcatcat tgccattccg tcggcgcctg
    22261 gcaagatcat ccgcgccgtc aacgatgcct gccgaaaaaa aggcatcccc tcgcgcacca
    22321 tgccagggat ttatgaattg ctcggtggca aggtcagtgt caaccgtttg cgcgaagtgg
    22381 atattaccga cctcctccgc cgtgaccatg tccgtgtgaa cgatgagcag gtagggcagg
    22441 cacttgaagg gaagcgggtg ctggtcaccg gggcgggcgg ctccattggc cgtgagttgt
    22501 gccgtcagat cgcccgtcgc aatccatctc aactgatttt gctcggtcat ggcgaaaaca
    22561 gcattttcga aattttgctt gaattgcaga acgactatgc cgcgttgaaa ctgatccctg
    22621 tcattgccga tatccgcaat ggacaacggc ttgattctgt atttgcccag catcatccgc
    22681 aggttgtgtt tcatgccgcc gcgcacaaac atgtcccatt aatggaagcc aaccctgtcg
    22741 aggccatcac caacaatgtg cttggcacac ggaatgtggt tcaggcggcg cttgatcaca
    22801 atgttgagcg atttatcctc atctccaccg acaaggcagt gaatccctcc agtatttatg
    22861 gcgccaccaa acgccttgct gaagcgatcg tcctcgatgc tgcacacaag aacaaacgtg
    22921 ccttcgccgt tgtccgcttt gggaatgtgc tcggcagccg tggcagcatc atccctattt
    22981 tcaaaaacca gatcgccaat ggcggccccg ttaccattac acaccctgat atgttccgtt
    23041 ttttcatgac catacctgaa gccgtgtatc ttgtgttaca ggcttcgtcc atgaacaacg
    23101 gcggcgaagt cttcgtcctc aacatgggtg aacccgtccg cattctcgat ctcgctgaag
    23161 acctcatcaa actctcaggc ctcgaaccgc aaaaggatat cgaaattgcc tttacaggca
    23221 tccgccctgg cgaaaaactg accgaagaac tttgggttga aggcacttct ctcattcaaa
    23281 ctccgcaccc cgatattttt cgactcgatc aagatgccgc atctgctctt aaccttgatc
    23341 tggttcaggc catcgagcaa ttatccaccc tcagcctcac caccgatgcc cagtccattg
    23401 cgaggcttct cgatcaactg atccctgggt ccaccatttt caacgcccca cacgactacc
    23461 tcgaagtctg acatgcctga agtctctctt tccgattgga atcaatttgt cgccagccat
    23521 cctgatgcgc atctgttgca gatgggccaa tggggtgaac tcaaaaatga ttttggatgg
    23581 aaacccgtcc gcctcatgct tgaccgtgaa acgggtgccc aaatcctctt ccgccgcctg
    23641 cccttgggct tgactctcgg ctacatgccg aaaccagttg acagtggacg gttgatggtt
    23701 gacagcgaat ctttttggcg tgaagtagat tcgatctgca aaaagaatca cgccatcttc
    23761 ctgaaaatcg aacccgattc atgggaagaa accttccaac cttccaacct tccaactttc
    23821 aaacgttcaa accataacat ccaaccccca cgcaccgtca cgctttccat caaagaagat
    23881 gaggaagaga tccttgcacg gatgaagccg aagtgccgct ataacatccg cctcgcggaa
    23941 aagaaaggca tcactgtccg cgcatggagt gacatctctg ccttccatga aatgatgacc
    24001 gtgacaggcg gacgggataa cttcggcgta cactcaaagg aatattatca acgagcctat
    24061 gagttgttcc accccatagg gacttgtgag ttgctcgtcg cagaattcga gggacgacca
    24121 ctggcctccc tgatggtctt ctccaatgca aaacgtgcct ggtatgtcta tggcgcttcc
    24181 aacgatcagg aacgcaaccg catgcccact tatcttttgc aatgggaagc cattcgctgg
    24241 gcaaaagcgc gcggctgtga agaatatgac ctgtggggtg tccctgacga acaagaagaa
    24301 accctcgaag caaattttga aacccgccgt gacgggcttt ggggcgttta tcgtttc
//
LOCUS       DAOQJC010000058        24117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_924_length_24117_cov_8.524811, whole genome shotgun sequence.
ACCESSION   DAOQJC010000058 DAOQJC010000000
VERSION     DAOQJC010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 24117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24117
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_924_length_24117_cov_8.524811"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..801)
                     /locus_tag="PLE39_10390"
     CDS             complement(91..801)
                     /locus_tag="PLE39_10390"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HNE68780.1"
                     /translation="MKKIYEHIHPIKFRLTVIALLCGATLLSVLLFRMRTLLSGSLDY
                     VFLLWNIFLAWIPLGFAHVATHFAWKRRYIVLSVPVAATLWLLFFPNAPYILTDLQHL
                     GSPKPGVPVWFDVLLIIWFAWTGLLLGMVSLFLMQDIVRREFGGIAGWFFVFAVGFLS
                     SLGIYIGRFLRWNSWDMFFNPLGRLTELLYYAAHPSMQSIIFISVFATFFIFFYTTIY
                     AFGRLLQEQAPKTSQETL"
     gene            complement(857..1555)
                     /locus_tag="PLE39_10395"
     CDS             complement(857..1555)
                     /locus_tag="PLE39_10395"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HNE68781.1"
                     /translation="MKNFLLRNRYKIAIFGLMTVASAICVFLVAARIAYSDSIRYTNL
                     VWNLFLAWIPFVLAYLAYTLSWKKWLLYFVIPVTTFLWLIFFPNAPYILTDLQHLAKE
                     STSAPLWYDVIVMVWFSWTGLLLGLVSLYLMHYIIQRMFGRAAGWAFVFIVSGLSSFG
                     VYLGRFVRFNSWDLLNDPKEIVVTILGLAIDPSMRVIAFTILFAVFYLFVYLTLYSFA
                     HLVREDPVAALASH"
     gene            complement(1561..2055)
                     /locus_tag="PLE39_10400"
     CDS             complement(1561..2055)
                     /locus_tag="PLE39_10400"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE68782.1"
                     /translation="MNKQDIQLLYRYNTWANARILDAVARVSAEQYLAADSYPHGGLH
                     GTLTHTLMAEWIWRQRWEGNSPTYFLKPEDFPTFEALRTRWQEEDRLLSAFLEALTDE
                     KLNAPFQFKTTRGVEMENILWQAMTHVVNHGTQHRSEAAAILTELGFSPGDVDLIVFL
                     RELK"
     gene            complement(2052..2927)
                     /locus_tag="PLE39_10405"
     CDS             complement(2052..2927)
                     /locus_tag="PLE39_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE68783.1"
                     /translation="MPFPYLDENRDLNESARQEAGGSFIKLTDGTTHFELVGPETAPA
                     IVLVHGFSVPYFIFDPTFDFLTKAGFRVLRYDLFGRGTSDRPQTAYNIHLFVRQLKEL
                     LAALNLPRVHLLGLSMGGPITAAFMDENPHSVEKHILIDPAGAKRVRLSLLLEAVKLP
                     IFGELALGLFGSVSLVKGIASDLFDPDLVEHFQARYKIQMQYKGFKQAILSTMRNQML
                     ESFFSTYERVGKLQKNTLLIWGKNDTTVPFENSMEILKAIPHAEFHAIEACGHIPHYE
                     KPEIVNKILMEFLEK"
     gene            complement(2976..5165)
                     /locus_tag="PLE39_10410"
     CDS             complement(2976..5165)
                     /locus_tag="PLE39_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNE68784.1"
                     /translation="MTNQSVGKSLPRIDAHDKVTGKALYSGDLVKDGMLHMKILFAER
                     PHARVKNINTGKADAAPGVVAVYTSKDVPVNEYGLQIPDQPVLCGPSKKPHTDIVRFV
                     GDQIAVVVARTEVEADAAIKLIQVEYEDLPILSDPKAALRPDAPILHPDRGDTNICVH
                     YKIRKGDMDAAFANADVIVEGEYHTPVQEHAYLQPEAGLSYIDDDGLITVEAGGQWTH
                     ADRETIAHSLGLPNEMIRIIYPAIGGAFGGREDLSVQIVLALATWKLQRPVKIIWSRR
                     ESMIGHGKRHAATVKAKWGATKDGKLIAAEMEMIGDGGAYMYTSNKVLGNAAITSTGP
                     YFIPNVKVDVYGVYTNNVPGAAFRGFGAPQALFMAESQMNKLAAHLGIDPVEFRLKNA
                     LRDGDAMGVGTPAPGPVSIVQCIEAARDQFGWKKSKAKKAAGKKSSPLVRGRGFAAGF
                     KNVGFSFGYQENCWAKVEIHGNGKMERAIVHHAGADVGQGMHTVMVQMAAHVLGLPMD
                     KVEIRTSDSAKQGNPGSASASRLTFMAGNSVKGAAEAALEKWKNEERPAVAEFKYLAP
                     RTTPFNKETGYSTPNFSYAYVAQAAEVEVDTETGHVRVLRLTSADDVGKAINPDLVVG
                     QIEGAVVQAHGYAVMEDYKTKDGRVLTDQFSTYLIPTILDIPEKVDSVLVEVPDPNGP
                     WGARGLGELPFLPVAPAIAAAIHDATGVWISEFPFTPERVLRALGKI"
     gene            complement(5172..5597)
                     /locus_tag="PLE39_10415"
     CDS             complement(5172..5597)
                     /locus_tag="PLE39_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68785.1"
                     /translation="MGKYNNNTTLKPSSIQSKGTHEIWRGFGCLMILIIPAISIASAY
                     ELIQYGIANKWPIPYELRGKVYLPEIFYSTPGLATIFSPISEVPNLLAIAAASLVLMM
                     LIGGVISVIYAAIYRAIGPSRYGPTDAPPSGIKVKKYKR"
     gene            complement(5616..7022)
                     /locus_tag="PLE39_10420"
     CDS             complement(5616..7022)
                     /locus_tag="PLE39_10420"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNE68786.1"
                     /translation="MWHEYINAISIEDVLKVLGEKKERARVVAGGTDLILELERGVRK
                     GIETLIDVSRIPGIDQITIDEDNVIHLGPRVTHNDCVASKVIRKHAYPLARAAWEVGA
                     PQIRNRGTVAGNLITASPANDTITPLMALGASVTLLSTRGSRTLPLKEFYTGVRRNVM
                     QPDEMLVDISFPAMKKNQRGTFIKLALRRAQAISLVNVAIIIDMKVDTVKSAVITLGA
                     VTPIITHAEDAEKYLAGKKLTEKNITHAADLATQAAHPIDDVRGSAAYRREMVKIITA
                     RGLRAIRDENEQAGMPKKPILLASTDVAKHPLQATSEFPTAPIETTINGKKYSFKQGH
                     DKTLLRLLREDGMLTGTKEGCAEGECGACTVFLDGKAVMACLVPAPRAHGAEIITVEG
                     LAKEEGTLHPIQETFIENGAVQCGYCTPGFLMSGAKLLEEKPNPTRNEIEQAITGNLC
                     RCTGYYKIVKAMEDASKL"
     gene            complement(7068..7592)
                     /locus_tag="PLE39_10425"
     CDS             complement(7068..7592)
                     /locus_tag="PLE39_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68787.1"
                     /translation="MSDFFEKVSSQIDPFKKLLSYIPGFKGYVERQTRRDADKELRDV
                     VARRFEEYWSRASNMQAELVSNGMLSYVDDMEKATLSMRTFIDKISRAARGYSGMFDA
                     IKINEKELEAIYQFDVAFFTLGEQVSSALDNVEASLNDESALPAAIRNLTTLSRLAVE
                     TFERRSEVVTGSGN"
     gene            7705..9123
                     /locus_tag="PLE39_10430"
     CDS             7705..9123
                     /locus_tag="PLE39_10430"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNE68788.1"
                     /translation="MSEHVKELLELVENSLAVDGAAVKVVDAAKFRKNVGKLVERSAL
                     GSDSTVGWSRYLVRAAALELGVYPASIHELYLARGRGEAPMTFTTPAFNLRALSYDAA
                     RAMFRAANKINAGAFIFELARSEMSYTAQRPSEYATNILAAAVAEGFVGPVFIQGDHF
                     QVSAKKYSADAEGELKAVRDLSIEAMNAGFFNIDVDTSTLVDIHLPTVPEQQKLNCEL
                     SAQLSAFIRENEPKGVTISIGGEIGEVGTNNSTEPELRAYMDGYNLEMKKLAPGKPGL
                     SKISVLTGTSHGGTVLADGSIAEVTIAFDVLRDLSRVARKDYGMGGTVQHGASTVAEE
                     NFNKFVQNEAIEVHLATNFATMFFDNIPADLKKEMYAWLDVNSAGDRKPGMTDEQFYY
                     KTRKNAIGPFKAKSYALSEAAKAKLGAAWEAQFDKLFNLLGMKDTKQYVEKYIPNVKV
                     APVLAHYVKEDVAAEDVSDLSD"
     gene            9152..9649
                     /locus_tag="PLE39_10435"
     CDS             9152..9649
                     /locus_tag="PLE39_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE68789.1"
                     /translation="MPKSDQGVTKDRYMLIPRTAIFLRRGDEYLLIKGAPTKRLWAGK
                     YNGLGGHVEQGEDVFTSARRELLEEAGLTADLWLCGTVIVDAGETGICLFVFCGENAQ
                     GEIKASAEGAVEWVRFDAVSHLPVVEDLPILLGKIHSMKHGDAPFSARSHYDENERLQ
                     VVFGV"
     gene            complement(9688..10455)
                     /locus_tag="PLE39_10440"
     CDS             complement(9688..10455)
                     /locus_tag="PLE39_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68790.1"
                     /translation="MYHTSKGVFIFFISIVLILTACSGGTESQSQQSGSSDSISGTCD
                     NPLLPVKQGAMWVYVSNGGPGGSFTYTDTVTSVLDGKFTIASQFADLIRTQEWACQSD
                     GLKALQLGGGTAAGISTLGMTAAFTTIEINGISLPKEVTPGMQWAYSLKMQGTMAMPG
                     DQQAQSSGVYSVTTQEIGRETITVPAGTFEAVKLQSNSTIDIVTSFEGIDVPIKFNGT
                     TISWYVPGVGFVKSVENGDFGGTTFAATTELQSYNIP"
     gene            complement(10532..11698)
                     /locus_tag="PLE39_10445"
     CDS             complement(10532..11698)
                     /locus_tag="PLE39_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE68791.1"
                     /translation="MTNTRTPAKIIPRAFPGIKPEEVQEIIINSRIQSYPPETIICRE
                     NEVENTFYMVLEGDFEVFKVINNTEIRILKALTAGDFFGEMALIHNAPRAATVKTKTN
                     VIVLELNKEGFDRVLKHSPSVAMAMVSEISNRLRQNDQLAIDDLRLRAAELADAYQKL
                     AEQELMRREFLSNIAHELRTPLQVASGYLQAMKKGMITSDQFNVAVDTIARNVDQITT
                     LANDLLFLQEIELVLPEFQSVNLVEVAMDVIKRYESKAQSRGVTIKVKGERGISNVRG
                     DAQSISRAVTALLDNAIKFSAPKSEVEVRFSEQRERVLISVEDRGIGIAPEIRPRIFD
                     RFFHLEKSGDELYSGLGIGLSITRQVILQHNGMLDVESQPGKGSKFTISLLKWQ"
     gene            11864..12874
                     /locus_tag="PLE39_10450"
     CDS             11864..12874
                     /locus_tag="PLE39_10450"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNE68792.1"
                     /translation="MYARTKFPARLLWMTLLASIFAGACQPALTESATVTPLPTFTPT
                     PYYTETPTPTSTPQPTPTPPRLVPNFTHIVIIIFENKEFGTVIGNNQMIYFNLLASSY
                     TLLTEHYAVTHPSLPNYLSLVSGDTFGITDNCEDCYINFLSLPDLIEQSGRTWKTYQD
                     DMPEPCFSGSTLRYVQKHNPFMYFDSIRLNPERCNRSVVPLTQLDEDIAANALPNFIF
                     ITPDICYSAHDCELELADGWLKENVQKLYLALEATKEPYLIILTWDEGQGTHSCCGLP
                     ELAGGRVATVLISPQVRQSFQDHTPYTHYSILKTISEAWALPFLGHAADANTAIIASP
                     WK"
     gene            complement(12880..13443)
                     /gene="rsmD"
                     /locus_tag="PLE39_10455"
     CDS             complement(12880..13443)
                     /gene="rsmD"
                     /locus_tag="PLE39_10455"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNE68793.1"
                     /translation="MSLRVIAGTARGRKLRSVPGDTTRPVMDRVKEALFNILADDVID
                     SNWWDLFGGTGAIGIEALSRGAAFVRFSDLNRAPIETIRENVTHCGFLQQSEIRRGDA
                     FSMLSGRADKSFEYIYIAPPQYKEMWLEALKLVDAHMDWLTDDGTVIVQIDPTEYQAV
                     ELENLEETEQRKYGSTLLVFYDHRIGE"
     gene            complement(13440..14414)
                     /locus_tag="PLE39_10460"
     CDS             complement(13440..14414)
                     /locus_tag="PLE39_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNE68794.1"
                     /translation="MTTHRERIQASIEGKKTDRTPIALWRHFPVDDQNPETLAASTLH
                     FQQTYDFDIVKVTPASSFAVKDWGVEDKWMGDSEGSRRYTKRVIHKPEDWEKLTVLDP
                     TSPHLAGQLTCLRLIRENLGPDTPLIQTIFNPLSQAKNLASNDTLVAHIRQHPEAVMK
                     GLETIARTTAKFIEAAAEIGIDGIFYAVQHAQANLLSVDEYTTFGLPYDQQTLVPVKN
                     LWCNMLHLHGKDVYFSLLRLLNFQIVNWHDRETHPSLAEAHSLFKGVLCGGLRQDTLV
                     LEDQAKIKEEAADAIQQTKSHRFILGTGCVVPITASHGNLLVARKSVE"
     gene            complement(14411..15970)
                     /locus_tag="PLE39_10465"
     CDS             complement(14411..15970)
                     /locus_tag="PLE39_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68795.1"
                     /translation="MPKVRQYPILILLIINIVIGLLTFRNYGLSWDEPLFYDYAHSLG
                     YAYSPKEWFSGNFDLENAFGSSGSDHANRGPAYILLAQPVVSVLESFKLDNASAWHLV
                     NFLTFQFGVYLFYRLSKRWVKESAALAATALFAWQPLLWGHAFINPKDPPFLVFFLGA
                     VCFGLEMVDALHTSQTTKFTLWRSTLLAAFFLGIATSIRVLGPLAGLLVCIYAFMQFG
                     QKGIPVLVKQLALYSALAILTAFITWPYLWGNPIQKFIEAFGFMSDNPTQLQVLFNGQ
                     LYRADELPRRYLPTLLVLTLTEPTWFLFAAGLLTALWHQVNITTRKITVLSSFILPTL
                     WFLIPFLYVILRKPPMYDGYRHFLFMLPPVFVFTGFAFEKLFEIIKSRQIAVALAALL
                     IAPAIYSGAQLHPYQYAYYNLFTGGTSGAFRTFETDYWLTCYKDAVEQFDQQNVQLFV
                     KREPIMASYYASENLNIRDYRAEFGEIRSGDFVLVNTRANEDIKTFKEAPVVLQIRRG
                     DAVFCEIKKIP"
     gene            complement(15978..16451)
                     /locus_tag="PLE39_10470"
     CDS             complement(15978..16451)
                     /locus_tag="PLE39_10470"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNE68796.1"
                     /translation="MTETLTPKSARHSRVTLSQLMHPEHANLLGNVHGGWIMKLVDEA
                     GALACMRHAQKKVVTVAVDSMVFREPIKIGDLIILNAEVTYTGRTSMEAEVQVIAENP
                     VSGERTHTNTAYLVYVALDDGGHPTTIPPLAIESEEEKQRMEQAQKRQAHRISQK"
     gene            complement(16460..16840)
                     /locus_tag="PLE39_10475"
     CDS             complement(16460..16840)
                     /locus_tag="PLE39_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68797.1"
                     /translation="MSTQKPVLIPIYTSKGDAEAFLLYPYLFNRLGDWIGFVNQKREV
                     YSVLGYFVGTLTNDPRIIGKRATSTLKPRQQPPPKPEKVFPPATVPLARMMPDLGPSA
                     VDILLDAPERLHTLDAGEQRQDLD"
     gene            complement(16843..17310)
                     /locus_tag="PLE39_10480"
     CDS             complement(16843..17310)
                     /locus_tag="PLE39_10480"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNE68798.1"
                     /translation="MILTNAKERTRFLKFAMVGTIGAVIDFGVMNLLSHFTDMSLVYA
                     GTISFICAVVSNFIWNRFWTYPESRSRPLINQLGMFFLVNVAGVVIRIPTLHYLEPPL
                     LHFFEGVFHASYATAEFYAKNITLASAVGIVMIWNFFVNRYWTYNDIDHSSEA"
     gene            complement(17307..18263)
                     /locus_tag="PLE39_10485"
     CDS             complement(17307..18263)
                     /locus_tag="PLE39_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE68799.1"
                     /translation="MRILITGAAGFLGSHLCDHLLKDGHEVIGMDNFVTGSPDNIAHL
                     ADNDHFSFYRRDVSEYIFVPGKVDAILHFASPASPNPQSKSGYFNLPIQTMKAGALGT
                     HNCLGLARAQNARFLLASTSEIYGDPEVHPQVESYTGNVDPIGTRAVYDEAKRFAESL
                     TMAYHRSHNVNTSIVRIFNTYGPRLDLEDGRALPNMIKQALLGQPLTVYGDGSQTRSF
                     CFVDDLIDGIVKLLYSEEHFPVNMGNPHERTILQFAETINKVTGNTAGIVFVDARSAR
                     DPQRRRPDITRAQTILNWEPKVSLEEGLIRSIPYFKQKLGLA"
     gene            complement(18287..19873)
                     /locus_tag="PLE39_10490"
     CDS             complement(18287..19873)
                     /locus_tag="PLE39_10490"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HNE68800.1"
                     /translation="MPRLRLRPHLLLFLLISIWLTGSCRSPQVGEQITVTINADGNAA
                     QVSVTAGSTVSQALQSAGIIPAKLDRSEPPLYTVLSNGDSITLTRVEEVFETEQVTVP
                     FERQIVRNETLPEGETRLVQAGVNGLLEVTYRRILENQVEISKTSVKTVVLTEALPEI
                     VMVGAQSSFTPLNIPGTLVYLAGGNAWIMEGSTANRRIIVSTGDLDGRIFKLSPNGEY
                     LIFTRKSKKPASQEINTLWAVRTKNIEPKPIWLQAYNVVHFAEWIPGTNSVAYSTVEP
                     RSTAPGWQANNDLYRVSLTGGSPRKMLEANSGGVYGWWGMSFAYSEDGRLAYARPDGI
                     GLVDQDGGYLKPMLGITPLNTHSDWAWIPPITWGSDGKTLYYVTHAPAPAPIVGEESP
                     FFDVAAISFENGASVSLIDAAGMFSYPAVSPSRWDGRQKDYQVAYLQSIFIEQSETSR
                     YRLMVMDRDGSNSRALFPPADTNGIEPQTPSWAPEPLEGQVGDFIAVTYQGNLWLIDS
                     GSGKAYQVTGDGLIVGIDWK"
     gene            complement(19891..20463)
                     /locus_tag="PLE39_10495"
     CDS             complement(19891..20463)
                     /locus_tag="PLE39_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68801.1"
                     /translation="MKTKIYHLTFLFAVLMLASLACNIFVGGPDYPTQTVPVSADEVQ
                     TMYTQIEQAFTAGAETGVVTLQITENQLTSLIALKLQEQTDPPFTDPQVLLRNGQLQM
                     YGKLASGSFNANMLITASVGIDETTGMPKIEVSSVDLGPIPAPEGLNAAINAMIAEAF
                     TGSLGPAATGFRLEAITIADGVMTMTGRIK"
     gene            20509..21090
                     /gene="scpB"
                     /locus_tag="PLE39_10500"
     CDS             20509..21090
                     /gene="scpB"
                     /locus_tag="PLE39_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNE68802.1"
                     /translation="MSDSQTTSASVSTPEQSLAAKIEAMLFVSAEPVPMAQLAAALDV
                     TASVVERGLKELDEALLNRGLRLQRNGGRVQLTTAPEMAVLIETFLGLEATTHLSRAA
                     LETLAIIAYQQPCTRPQVDAVRGVNSDGMLKSLLSKGLVQEAGRTDGPGRPILYSTTP
                     EFLQHFGLGSILELPPLSAPTEPDNEHSELLKG"
     gene            complement(21162..23480)
                     /locus_tag="PLE39_10505"
     CDS             complement(21162..23480)
                     /locus_tag="PLE39_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNE68803.1"
                     /translation="MAKDIKSIISKMTLEEKAAICTGASNWSTTPVERLGIPEMIVSD
                     GPHGVRRVADVRSMGQKSLPATCFPTASCTASTWDVDLIKQMGIALAEECIALNVDVL
                     LGPGVNMKRSPLNGRNFEYFSEDPFLAGELAANFINGIQSKGVGTSLKHYAANNQEFQ
                     RFSISAEIDERTLREIYLPAFEKAVKQAQPWTVMCSYNKVNGTFASEHQQLLTEILKE
                     EWGFEGLVVSDWGAVRNRVSALKAGLDWEMPGPQDLRVKAVVEAVRSGDLSEATLDES
                     VRRILRIVFKAKETAKGNGTFDVDAHHELASKIATEGVVLLKNDGLLPLKDHQHIAVI
                     GRSAETAHFQGGGSSHINPTKVAMPYKELQARAENAELTYAEGYPTDNSFQQGMIDDA
                     VKLAQSADVALLYIALPTFKESEGYDRHDLDLTLQQIALIKAVSKAQPKTVVVLNNGA
                     PVAMSEWIDDVSAVLEGWMMGQAGGAALADILFGRVNPSGKLAETFPLKLTDTPSHIN
                     WPGGAGKVHYGEGIFIGYRYYDAKEIPVLFPFGHGLSYTTFEYSNAKVSSNNFKDVDG
                     LTVTVDVKNTGNLAGKETVQVYVHDQKSDLIRPVKELKGFAKVELQPGETKTVSIHLD
                     FRAFAYYHPEHKQWITEDGGFDILIGASSADIRSRLTTTLQSTLDLPCILDIESTLRE
                     WMADRRGRQVLGSLYEQIENQVRRLFAGEDRYGNTKSGEQVTEQTVMGMDIMDMMVDM
                     PVVSVLMFQKESLTMPAEEMVAGLLRQVHSMK"
     gene            complement(23639..>24117)
                     /locus_tag="PLE39_10510"
     CDS             complement(23639..>24117)
                     /locus_tag="PLE39_10510"
                     /inference="COORDINATES: protein motif:HMM:NF025668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HNE68804.1"
                     /translation="IPVHVGQQPIFYSQVRGQHGSRYADLTQEPLFPFGHGLSYTKYT
                     YSNLRLSSSTLAQGDAVKVLVDVENIGQREGVEIVQVYVSDVVTSVTWVNKALKGFAR
                     VQLQPGEKKTVEVELPWESFRIVNTKAEYIVEPGEFEILVGPSSRDQDLLKTTLTI"
BASE COUNT         5562 a         5868 c         6653 g         6034 t
ORIGIN      
        1 caagaatgac gacttgaaaa cctgtgcgat gcaggcgcaa tgcgacaccg cttgcaaggt
       61 cgccgccgcc gcgaaggaca ataagatggg tcatagagtt tcctgtgaag ttttaggggc
      121 ctgttcctga agcaaacggc caaaagcgta aatggtggtg taaaagaaaa tgaagaaggt
      181 tgcgaacacg ctgataaaaa tgatggactg catgctgggg tgagcggcgt aataaagtaa
      241 ttcggtcagc cgcccaagcg ggttgaagaa catatcccat gaattccagc gcaggaacct
      301 gccaatgtaa ataccaaggc tgctgagaaa cccaaccgca aacacaaaga accagcctgc
      361 aatgccgcca aactcacggc ggacaatatc ctgcatgaga aacagcgaaa ccatgccaag
      421 caggagacct gtccatgcaa accagatgat caacagcaca tcaaaccaaa caggcacacc
      481 tggtttggga gagccgaggt gctgcaaatc ggtcagaatg tagggagcgt ttgggaaaaa
      541 caacaaccag agagtcgccg ccacaggcac gcttaagacg atgtatctgc gcttccatgc
      601 aaagtgagtg gccacgtgtg cgaacccaag cggaatccat gcgaggaaga tgttccagag
      661 cagaaacaca taatccagtg atccgcttaa aagggtgcgc atgcgaaaga gcaggacgga
      721 aagcagggtg gctccacaca acagcgcaat gaccgtcaag cgaaacttga tgggatggat
      781 gtgctcgtaa attttcttca tggttttgac ctccagtttt gaggccgatt atatgaatac
      841 cgacttccaa acgctttcaa tgactcgcaa gtgcggcaac aggatcttca cggacaagat
      901 gtgcgaagga atacaaggta agatagacga acagataaaa gaccgcgaac aggatcgtga
      961 aggcgatcac ccgcatggat gggtcaatgg caaggccgag gatggtgacg acgatttcct
     1021 ttgggtcgtt gagcaggtcc caggaattga aacggacgaa acgacccaga tacacaccga
     1081 aactgctcaa ccctgaaaca atgaatacga aggcccagcc tgcggcgcgc ccaaacatgc
     1141 gttggataat gtagtgcatc aggtacagtg aaaccagacc gagcaacagt cctgtccacg
     1201 aaaaccagac catcacaatc acatcatacc aaagcggcgc gctggtggat tcttttgcca
     1261 ggtgctgtaa atcggtgaga atgtatggcg cattggggaa gaagatcagc cacaaaaacg
     1321 tggtgacagg gataacgaaa tacaacagcc actttttcca cgaaagcgtg taggcgagat
     1381 acgccaacac gaacggaatc cacgcgagga agagattcca caccaggttg gtgtagcgga
     1441 tcgaatcgct gtaggcaatg cgcgccgcca caaggaagac acaaattgcc gaggcgacag
     1501 tcatcaagcc gaagatggcg atcttgtaac ggttgcgaag caaaaagttt ttcatggctt
     1561 ttatttcaac tcacgcaaaa acacgatcaa gtccacatca ccgggggaga atcccaactc
     1621 ggtgagtatg gcagcagctt cactgcggtg ctgggtgccg tggttgacca catgtgtcat
     1681 cgcctgccag aggatgtttt ccatttccac accgcgggtg gttttgaatt ggaagggcgc
     1741 attcagcttt tcatctgtca atgcttcaag aaaagcgctc aagagtcggt cttcttcctg
     1801 ccagcgtgta cggagcgctt caaaggtggg gaagtcttcg ggcttgagaa aataggtcgg
     1861 cgaattgcct tcccaacgct ggcgccagat ccattcggcc atgagggtgt gggtcaatgt
     1921 gccgtgcagc ccgccatgag ggtacgaatc ggcggcgaga tattgctcag cgctgaccct
     1981 ggcaacagca tccaaaatac gggcgttggc ccaggtgttg tatctgtaaa gaagttgaat
     2041 atcttgtttg ttcatttttc taaaaactcc atcaatatct tgttgacgat ctctggtttt
     2101 tcgtagtgcg gaatatgtcc acaggcttcg atggcatgaa attctgcatg gggaatggcc
     2161 ttgagaattt ccatgctgtt ttcaaagggc acggtcgtat catttttacc ccagatcagc
     2221 agggtatttt tttgcagttt ccccacgcgc tcgtaagtgg agaaaaacga ttcaagcatt
     2281 tgatttcgca tcgtcgaaag gatcgcctgc ttgaaacctt tgtactgcat ctggatctta
     2341 taccgcgcct gaaaatgctc gaccagatct ggatcgaaca ggtctgaggc aatgcccttg
     2401 accaaactca cgcttccaaa cagaccgagg gccagttcgc caaaaatggg cagtttcacc
     2461 gcttcgagca gaagtgaaag ccgcacccgt tttgcgccag cagggtcgat caggatgtgt
     2521 ttttcaacgc tgtgcggatt ttcgtccatg aaggcggcag tgatggggcc gcccattgaa
     2581 agaccgagca ggtgaacccg cggcaggttt aacgcagcaa gcagttcttt gagctgacgt
     2641 acgaacaggt gaatgttgta tgcggtttgc ggcctgtcgg atgttccgcg cccgaacaaa
     2701 tcataacgca agacgcggaa acctgctttt gtcaaaaagt cgaatgtggg gtcgaagatg
     2761 aagtatggga cggagaatcc gtggacgagc acgatggcag gcgctgtttc ggggccgacc
     2821 aattcaaaat gggtcgtgcc atctgtcagc ttaatgaagg aaccgcccgc ttcctggcgg
     2881 gctgattcgt tcaagtctct gttttcgtcg agatagggga agggcatggt ctttgcaggg
     2941 atacagcggg aactgtatcc ctgcgaggtg gttgtttata tttttccaag tgcgcgcagg
     3001 acgcgttccg gggtgaaggg gaattcgctg atccaaacgc cggtggcatc gtgaatggcg
     3061 gcggcaatgg cgggagcaac aggcaggaag ggcagttcgc ccaggccgcg cgctccccat
     3121 gggccgttgg ggtctggcac ttccacgaga acggaatcca ctttttcggg aatgtctaaa
     3181 atggtgggga tgagataggt gctgaattgg tcggtgagga cgcggccgtc tttggttttg
     3241 taatcttcca tcacggcgta cccatgagcc tgtaccaccg caccttcgat ctgcccgacg
     3301 acaaggtcag ggttgatggc tttgccgaca tcgtcggctg aggtcaggcg taatacgcgc
     3361 acatggcctg tttcagtgtc cacttccact tcggcggctt gggcaacata ggcgtaggag
     3421 aaattcgggg tggagtagcc tgtttctttg ttgaatggcg tggtgcgcgg cgcgaggtat
     3481 ttgaactctg caacagcagg gcgttcttcg ttcttccatt tttcgagggc ggcttcagcg
     3541 gcgcctttga ccgaattgcc tgccatgaag gtcaggcgtg atgcagaggc ggaaccaggg
     3601 ttgccttgtt tggctgaatc agaggtgcgg atttcgacct tgtccatggg caggccgagt
     3661 acatgcgctg ccatttgaac catgaccgtg tgcattccct gaccgacatc ggcacccgca
     3721 tggtggacga tggcgcgttc catttttcca ttgccgtgaa tctcaacctt ggcccagcag
     3781 ttttcctgat agccaaaaga gaagccgacg tttttgaagc cagcagcgaa tcctctacca
     3841 cgcacgagcg gggacgattt tttgccagcg gcctttttcg ctttcgattt tttccagcca
     3901 aactgatcgc gggcagcttc aatgcattgt acgatgctga ccgggcccgg ggcgggtgta
     3961 ccaacgccca tggcatcgcc atcgcgcagg gcatttttca ggcggaattc cacagggtca
     4021 atgcccagat gtgcagcaag tttgttcatt tgcgattcgg ccatgaacaa agcctgtggg
     4081 gcgccgaatc cgcggaaggc cgcgccgggg acattgtttg tgtacacgcc gtacacatcc
     4141 acttttacgt tggggatgaa gtatggcccc gtggaggtga tggcggcatt gccaagcacc
     4201 ttattggagg tgtacatgta ggcgccgcca tcgccgatca tttccatttc tgcggcgatg
     4261 agttttccat ctttggttgc accccatttg gctttgactg tggcggcgtg ccgcttgccg
     4321 tggccgatca tggattcgcg gcgtgaccag atgattttta ccgggcgctg tagtttccat
     4381 gtggcaaggg ccagcacgat ctgcactgaa aggtcttcgc gcccgccgaa tgccccgcca
     4441 atggctgggt agatgatgcg gatcatttca ttgggcaggc cgagtgaatg ggcgatggtt
     4501 tcgcggtcgg catgagtcca ctggccgcct gcttccacgg tgatgagccc gtcgtcgtca
     4561 atgtaagaga ggcccgcttc aggctgcagg taggcgtgtt cctgtacggg ggtgtggtat
     4621 tcaccttcca cgatgacatc tgcatttgcg aaggccgcgt ccatgtcacc tttgcggatc
     4681 ttgtagtgga cgcagatgtt ggtgtcgcct ctatcggggt ggaggatggg tgcatcggga
     4741 cgaagggcag ctttcgggtc ggaaagaatc ggaaggtctt catattcgac ctgaatcaac
     4801 ttgatcgccg catccgcctc gacctcggtc ctggctacca cgaccgcgat ctggtcacct
     4861 acaaaacgga caatgtcggt gtgtggtttt ttggatggtc cgcacagaac gggctggtcg
     4921 ggaatttgca atccatattc gttgaccggt acatcttttg aagtgtatac ggctacgaca
     4981 cccggtgcgg catctgcctt gccggtgttg atgtttttta cgcgcgcatg tggacgctct
     5041 gcgaagagta tcttcatgtg cagcataccg tcttttacaa ggtcgcctga gtagagggct
     5101 ttgccggtta ccttgtcatg ggcatcgatt cttggcaatg atttgccgac tgattgattg
     5161 gtcatggttt cctagcgctt gtactttttt actttgattc cggagggagg ggcatctgtc
     5221 ggtccatagc gggatgggcc aatggcgcga tagatcgcgg cgtagatgac cgagatgacg
     5281 ccgccaatga gcatcatcag aaccaggctg gctgctgcaa tggcaagcag gtttggaact
     5341 tcgctaatgg ggctaaagat cgttgccagc ccgggggttg agtagaaaat ctcgggcagg
     5401 tacactttgc cgcgcaattc gtagggaatg ggccatttgt ttgcgatacc atattggatc
     5461 aactcatagg ctgatgcaat tgatattgca ggaatgatca agatcatcaa acatccaaag
     5521 ccgcgccaga tctcgtgcgt tcctttggat tggatgcttg atggcttcaa ggttgtgttg
     5581 ttattgtatt tgcccatcag aatttctcct acctgttaaa gtttgcttgc atcttccatt
     5641 gctttgacga tcttgtaata gcctgtgcag cggcagagat ttccggtgat ggcttgttcg
     5701 atctcgttgc gggttgggtt gggcttttct tccagcaatt tggccccaga catgaggaag
     5761 ccgggggtgc aatatccaca ttgcacggca ccattttcaa tgaatgtttc ctgaatggga
     5821 tgcagggtac cttcttcttt ggcaagccct tccacggtga tgatctctgc gccatgtgcg
     5881 cgtggtgcag ggacgagaca ggccatgacg gcctttccat cgaggaaaac ggtgcaggcg
     5941 ccacattcgc cttcggcgca accttctttt gtgccggtca acatgccgtc ttcgcgcaac
     6001 agacggagga gggttttatc atgaccttgt ttgaaggaat atttcttccc attgatggtg
     6061 gtttcgatgg gggcggttgg gaattcactg gttgcctgca gggggtgttt tgcaacgtca
     6121 gtgcttgcca gcaaaatcgg cttctttggc atgccagcct gttcattttc gtcgcggatg
     6181 gcgcgaagtc cgcgggcggt gatgatcttg accatctcgc ggcggtaggc agcggagccg
     6241 cgcacatcgt caatggggtg tgcggcttgt gtggcaagat cggcggcgtg ggtgatgttt
     6301 ttttcggtga gtttctttcc cgcgaggtat ttttcggcat cttcagcgtg ggtgatgatg
     6361 ggagtcactg ccccaagggt gatgactgcc gatttgacag tatccacttt catgtcaatg
     6421 atgatcgcga cgttcacaag cgaaatcgcc tgtgcgcggc gcagggcaag tttgatgaag
     6481 gtcccgcgct gattcttttt catcgctggg aaggaaatat ccaccagcat ttcatcaggc
     6541 tgcatgacgt tcctgcggac gcctgtgtag aactctttga gtggaagggt gcggctgccg
     6601 cgtgtggaga gcagggtaac gcttgcgccg agtgccatca acggggtgat ggtgtcgttg
     6661 gcgggtgagg cggtgatgag gttgcctgcc acggtgccgc gattgcggat ctgcggtgcg
     6721 ccaacctccc aggcggcgcg ggccagcggg taggcatgtt tgcggatcac cttggaagct
     6781 acacaatcat tatgtgtaac ccgtggccca aggtggatca cgttatcttc gtctattgtg
     6841 atttggtcaa taccagggat tcgtgacaca tcgatcagtg tttcaattcc cttgcggaca
     6901 ccgcgttcga gttcgaggat gaggtcggta cctccagcta cgacccgggc gcgttccttt
     6961 ttttccccta gaaccttgag tacatcttca atggatattg cgttgatata ttcgtgccac
     7021 atagttaatg ttatagggac acggcgccgc cgtgtcccta ccataaatta attcccgctt
     7081 ccggttacaa cttcggagcg gcgttcaaac gtttccacgg ccagtcttga aagtgtggtc
     7141 aggttgcgga tggcagcggg cagggcgctt tcatcgttca gactcgcttc cacattatcc
     7201 agtgcgctgc tgacttgttc tccaagtgta aagaaggcaa cgtcgaactg gtagatggct
     7261 tcaagttcct tttcgttgat cttaatcgca tcgaacatgc ctgaataacc gcgtgcagcc
     7321 cttgaaattt tgtcaataaa ggtgcgcatg gaaagtgtgg ctttttccat atcgtccacg
     7381 tagctgagca tgccgttgct gacaagttcg gcctgcatgt tcgaagcacg actccagtat
     7441 tcctcgaagc ggcgggccac cacatcacgc agttctttgt ctgcgtcgcg gcgggtttgt
     7501 ctttcaacat atcccttgaa ccctgggatg tacgaaagaa gtttcttgaa cgggtcgatc
     7561 tgactactta ctttttcaaa aaaatcactc atgtgagcct cctgccatag tatacgtagg
     7621 tcttgccaaa tcgtctcacg attttttata ttatatcgtt tatttccagt tataatcaag
     7681 ttatcatttt ttcaggagtt aactatgtct gagcatgtca aagaattgtt ggaattagtt
     7741 gaaaatagcc tggcagtgga tggggcggct gtcaaggtgg ttgatgcggc gaaattcagg
     7801 aagaatgtgg ggaagctggt ggaacgctct gcactcggtt cggattcaac tgtgggctgg
     7861 tcccgttacc ttgtccgtgc agccgccttg gaattgggcg tgtaccctgc ttccatccat
     7921 gagttgtacc ttgctcgcgg acgcggcgaa gcaccgatga catttaccac ccccgcattc
     7981 aatttacggg ccttgtcgta tgatgcggcg cgggcgatgt tccgtgcggc aaacaagatc
     8041 aatgcgggcg cgttcatttt tgagttggcg cgttctgaaa tgagctatac cgctcagcgg
     8101 ccatccgagt atgccaccaa catcctcgcg gcggcagttg ctgaaggatt tgtcgggcct
     8161 gtgttcattc agggcgatca ctttcaggtt tcggcgaaga aatattcggc cgatgccgag
     8221 ggtgagttga aagcggtccg cgatctttcc attgaagcga tgaacgcggg ttttttcaat
     8281 atcgatgtgg atacttccac gttggtggat attcatcttc cgaccgtgcc tgagcaacag
     8341 aaattgaatt gtgaactttc tgctcagctt tccgcgttta tccgcgagaa tgagcccaaa
     8401 ggcgtaacca tctccatcgg tggtgagatc ggcgaagtag gcacgaacaa ctccaccgaa
     8461 cccgaactcc gcgcttacat ggacggctac aaccttgaaa tgaagaaact tgcccccgga
     8521 aagcctggct tgagcaagat cagcgtgttg actggcactt cacatggcgg cacggtcctt
     8581 gccgatggtt ccattgctga agtcaccatt gcttttgatg tgttgcgtga tctcagccgt
     8641 gttgcccgca aggattatgg catgggcggc acagttcaac atggcgcttc gaccgtggca
     8701 gaagagaatt tcaacaaatt cgttcagaac gaagccatcg aagtgcatct tgccacgaat
     8761 tttgccacga tgttcttcga taatattcct gcagacctca agaaggaaat gtatgcctgg
     8821 ttggatgtca actctgcagg tgaccgcaaa cctggcatga ccgacgaaca gttctattac
     8881 aagacccgca aaaatgcgat cggaccgttc aaggcgaagt cgtatgccct ttctgaagcg
     8941 gccaaagcca aactcggcgc agcgtgggaa gcgcagttcg ataagctttt caacctgctg
     9001 ggcatgaaag atacgaaaca gtatgtggag aagtacattc ccaacgtgaa ggttgccccg
     9061 gtgctggcac attatgtaaa ggaagatgtc gctgctgaag atgtaagcga cctctcggat
     9121 taatccccca ttctgccttg aacgaagaga aatgccaaaa tcagaccaag gcgtaaccaa
     9181 agatcgatat atgctcattc cccgcacggc gatttttctc cgtcgcgggg atgagtattt
     9241 attaattaaa ggggctccca caaaacgatt gtgggcaggg aagtacaacg gtcttggcgg
     9301 tcacgtggaa caaggcgaag acgtgttcac ctctgcgcgg cgtgaattgc tcgaagaggc
     9361 ggggctgact gctgatttat ggctgtgcgg cacggtgatc gtggatgccg gggaaacagg
     9421 aatctgtctg tttgtgtttt gtggagagaa tgcccaaggg gagatcaagg ccagtgcaga
     9481 aggagcagtt gaatgggtcc gctttgacgc ggtttctcat cttcccgtgg tggaagacct
     9541 gcccatcctg cttgggaaga tacacagtat gaagcatggt gatgcgccct tttcagcgcg
     9601 gtcacattat gatgaaaatg aaagactaca ggttgtattt ggtgtttaga acgcacgggg
     9661 aaagctggtg aatttgaaga gttctcgtta cggaatgtta tagctttgta gttctgtggt
     9721 ggccgcgaag gtggttccac caaaatcacc gttttcaaca gatttgacga agcccacgcc
     9781 cggcacatac caactgatgg ttgtgccgtt gaatttaatg gggacatcaa tcccttcaaa
     9841 acttgtgacg atatcaatgg ttgagttgga ttgcaattta accgcttcaa acgtgccagc
     9901 tgggaccgtg atggtttccc gcccgatctc ctgtgtggtc acagaatata ccccgctgga
     9961 ttgcgcctgc tggtcgccag gcatcgccat ggttccctgc attttcaggc tataggccca
    10021 ttgcatgcct ggggttactt ctttggggag actgatcccg ttgatttcaa tggttgtgaa
    10081 ggctgcggtc attccaagtg tggaaattcc tgcggctgtg ccgccgccaa gttgtaacgc
    10141 cttcaggccg tcgctctgac aggcccattc ctgtgtgcgt atcaggtctg cgaattgtga
    10201 tgcgatcgtg aacttgccgt caagtacgga tgtgaccgta tctgtgtatg tgaatgaacc
    10261 gcctgggccg ccgttgctga cataaaccca catggcgcct tgtttgacag ggaggagggg
    10321 gttgtcacat gttccactga tgctgtcaga ggaaccagat tgctgagatt gtgattctgt
    10381 tccaccactg catgcagtta agatcaaaac gatcgagatg aaaaaaatga atacgccctt
    10441 gctggtatgg tacatgcttg ctccgatgtt tatctcaggc agcgattcca aggatgtagg
    10501 ctgcgggaat gtgcctgtcg tgcccgttct tttattgcca cttcaacagg gagatcgtaa
    10561 atttgcttcc tttgcctggc tggctttcca catccaacat gccattgtgt tgaagaatta
    10621 cctgacgggt gatggaaagt ccgatgccga ggccgctgta taactcgtcg ccgctttttt
    10681 ccaaatggaa gaaccgatca aaaatgcgcg gacgaatttc gggggcaata cctatgccgc
    10741 ggtcttccac ggaaatgaga acgcgttccc tttgttctga aaaacgcact tccacctcac
    10801 tcttcggtgc gctgaatttg atggcattat ccagcagggc agtgacagcc cgtgaaatgg
    10861 attgcgcatc gccgcgtacg tttgaaattc cacgctcacc cttgaccttg atggtaacac
    10921 cacgggactg ggctttactt tcatagcgtt tgatcacatc catggccact tcaacaagat
    10981 tcaccgattg gaactcaggc agaaccaatt cgatctcttg aagaaatagc aggtcgtttg
    11041 ccagtgtggt gatctggtct acgttgcggg cgatcgtgtc cacggcaaca ttaaactgat
    11101 cgcttgtgat cattcccttt ttcatggcct gcaggtaacc gctggctacc tgtaaaggtg
    11161 tgcgcaattc atgggcaatg ttggaaagga attcgcgccg cattaattcc tgctcggcga
    11221 gtttttggta ggcatctgcc aattccgcag cgcgcaggcg taaatcatca atggcaagct
    11281 ggtcgttttg gcgcaggcgg ttgctgatct cactcaccat ggccatggca acgctggggc
    11341 tgtgttttag aacacgatca aagccttcct tattaagctc aagcacgatc acatttgtct
    11401 tggtctttac ggtggcggca cgcggcgcat tatggataag cgccatttcg ccgaaaaaat
    11461 cacctgcggt caacgctttc agaatgcgta tttccgtatt gttgatcacc ttgaatactt
    11521 caaaatctcc ctcaagtacc atgtaaaaag tattttcaac ctcgttctcc cgacaaataa
    11581 tggtttcagg cgggtaagat tgaatgcgac tattaataat gatttcctgc acttcttcag
    11641 gcttgatacc agggaacgcg cgcggaatga ttttggctgg tgtgcgtgta ttggtcatgg
    11701 atttactcta tggattctcg aatgcaattt cctgatttta tcatgcacag aaattgcatc
    11761 cttcacccat tcggactaca agtaaatttt agcaaaaaat attcgcaaac acatgtctga
    11821 aatattacat tttcgaaagg gattttgctc tataatccac caaatgtatg ccagaacgaa
    11881 atttcctgcc cgccttcttt ggatgacgct tctcgcatcc atcttcgccg gagcctgcca
    11941 gcccgcattg acagagtcag caacggtgac gcctctccct acttttaccc ccaccccgta
    12001 ttacaccgaa acacctacac ccacatccac accccagcca acccccacgc caccgcgtct
    12061 ggtcccgaat ttcacccata ttgtcattat tatttttgaa aacaaggaat tcggtaccgt
    12121 cattggcaac aaccagatga tttacttcaa tttgcttgcc agttcttaca ccctgttgac
    12181 cgagcattat gctgtgactc atcccagcct gccgaattac ctttccctgg tcagcggcga
    12241 taccttcggc attaccgaca attgcgaaga ttgttacatc aattttctca gcctgcctga
    12301 tcttattgaa caaagtggac gaacctggaa aacctatcaa gacgacatgc ccgagccgtg
    12361 tttctcaggc agcaccctgc gttatgtgca aaagcacaac ccattcatgt attttgattc
    12421 gatccgcctg aacccggagc gctgtaatcg cagtgtcgtc ccgttgacgc agctggatga
    12481 agatattgcg gcaaatgcac tgcccaactt catcttcatc acccctgata tttgttactc
    12541 cgcccacgat tgtgaattgg aacttgctga tggctggctg aaggaaaacg ttcaaaaact
    12601 gtatctggca cttgaagcca caaaggagcc ctaccttatc atccttacct gggatgaagg
    12661 gcagggcact cattcctgtt gtgggcttcc tgagcttgca ggtggaaggg tggccacagt
    12721 tctaatatct ccgcaggtgc ggcaaagttt tcaagatcac accccataca cacactattc
    12781 cattttgaag accatctccg aagcctgggc attgcctttt ctcggccatg cagcagatgc
    12841 caatactgcc atcatagcct ctccgtggaa gtgatttctt tattcgccaa tacggtgatc
    12901 gtaaaagacc aaaagcgtac tgccgtattt tctttgctcg gtctcttcga ggttttccag
    12961 ttccacagcc tgatactcgg ttgggtcaat ttgtacaatg acagtcccgt cgtctgtcag
    13021 ccaatccatg tgggcatcca ccagttttag ggcttccaac cacatctctt tatactgtgg
    13081 tggagcaatg tagatgtatt caaaactttt atcggccctg cctgaaagca tgctgaaggc
    13141 gtcaccccgc cgtatctcgc tttgttgtaa aaacccgcag tgtgttacgt tctctcgtat
    13201 ggtttcgatg ggagcgcggt tgagatccga gaaacgtaca aaggccgcgc cgcgactcaa
    13261 ggcttcgatg ccgattgcgc ctgtgccacc aaatagatcc caccaattag aatcgatcac
    13321 gtcatcggca aggatattga acagtgcctc tttcacacga tccatcaccg gacgtgtggt
    13381 gtcgccaggc accgatctta gttttctgcc ccgcgcggtt ccagcaatga ctctcagact
    13441 cattcaacgc tctttcttgc aaccagcaaa tttccgtgtg atgcggtgat gggaaccacg
    13501 caccctgtgc caaggatgaa acgatgactc ttcgtctgct ggatggcatc cgccgcttct
    13561 tccttgattt tggcttgatc ctccagaaca agagtatctt gcctcaagcc accgcacaat
    13621 actcctttga agaggctatg ggcttcggcc agggatggat gtgtttcgcg gtcgtgccag
    13681 ttgacgattt ggaagttgag cagacgcagc aaggaaaagt aaacgtcttt gccgtgcagg
    13741 tgaagcatat tgcaccaaag gtttttgaca ggaaccagtg tctgctggtc gtaaggcagg
    13801 ccaaaggttg tgtactcatc cacagaaagc aggttggctt gggcgtgctg gacagcataa
    13861 aaaataccgt caatgccgat ctcagccgcg gcttcgatga actttgccgt ggtcctggcg
    13921 atggtttcaa gtcccttcat gacggcttca gggtgctgcc tgatgtgtgc cacaagggtg
    13981 tcattgctgg cgaggttttt cgcctgcgaa agcggattaa agatggtttg gatgagcgga
    14041 gtgtctgggc cgagattctc tcgaattaaa cggaggcagg tcagttgtcc cgcgagatgc
    14101 ggtgaagttg ggtccagcac ggtgagcttt tcccagtctt caggtttgtg gatgacacgt
    14161 tttgtgtatc gtcttgaacc ctcggagtcg cccatccatt tatcctccac tccccaatct
    14221 ttgactgcaa aggaggaagc gggtgtgact tttacgatgt caaaatcata ggtttgttga
    14281 aaatgcaacg tggaagccgc aagggtctcg gggttctggt catccacggg gaagtgccgc
    14341 cagagggcaa tgggtgtgcg gtctgttttt ttgccttcaa tactggcctg gatacgttct
    14401 cgatgtgtgg tcatgggatt ttttttatct cgcagaatac cgcgtcaccg cggcggattt
    14461 ggaggacgac aggcgcctct ttgaaagttt tgatatcttc atttgcgcgt gtgttgacga
    14521 ggacaaaatc gcctgatcgg atctcgccaa actcggcgcg gtaatcacga atgttgaggt
    14581 tttcactggc ataatacgaa gccatgatcg gctcgcgctt gacaaagagt tgtacgttct
    14641 gttggtcaaa ttgttcaact gcatctttgt agcaggtgag ccagtaatct gtttcaaagg
    14701 tgcggaatgc gccgcttgtg ccgccagtga acagattgta atacgcatat tgataggggt
    14761 gaagttgtgc cccgctgtat attgcaggtg caatgaggag ggcggcaagt gcaacggcaa
    14821 tttggcggga tttgatgatc tcaaataatt tctcgaaggc gaaccctgtg aaaacaaaaa
    14881 ctggcggcaa cataaacaaa aaatggcggt agccatcata cattggcggc ttgcggagga
    14941 tgacgtataa aaatggaatg agaaaccaaa gcgttggcaa gataaaagaa gaaagaacgg
    15001 ttattttacg agttgtgata ttgacctgat gccaaagtgc cgtcaacaga cccgcggcaa
    15061 aaagaaacca tgtgggttcg gtgagggtga gaacgagcaa ggtgggcagg tatcgacggg
    15121 gtaattcgtc ggcacggtac agttgaccgt taaacagcac ctgcaattga gtagggttgt
    15181 cggacatgaa cccgaaggct tcgataaatt tttgaattgg atttccccac aaatatggcc
    15241 aggttataaa ggcggtcaaa atggcgaggg cagaatacag tgcgagttgc ttcaccaata
    15301 caggtattcc tttttgcccg aattgcatga aggcgtaaat acagaccagc agcccagcca
    15361 gcggccccaa cacacggatg gatgtggcaa tgccaaggaa aaaggccgca agaagtgttg
    15421 acctccacaa tgtgaatttt gtggtttggc tggtgtgaag tgcatccacc atctccaggc
    15481 caaaacaaac ggccccgaga aaaaaaacga ggaacggcgg gtctttcggg ttgataaaag
    15541 cgtgtcccca aagaagaggc tgccatgcaa ataaagcggt ggcagccaat gcagcagatt
    15601 cttttaccca gcgttttgag agacggtaaa aaagataaac gccaaattga aatgtaagga
    15661 aattgaccag atgccatgca gaagcattgt ccaatttgaa tgattcaagc acagatacaa
    15721 caggttgcgc cagcagaatg taggcgggcc cgcggttggc atgatctgac ccgctggagc
    15781 caaaggcatt ttcgaggtcg aaatttccgc tgaaccattc ttttggagaa tacgcatatc
    15841 caagtgaatg ggcgtaatca taaaagagcg gctcatccca ggataagccg taattacgaa
    15901 atgtgagcag cccgatcaca atgtttatga tgagcagaat cagaatgggg tactggcgga
    15961 cttttggcac gttggtttta tttttgcgaa atgcggtggg cctgtcgctt ttgggcctgt
    16021 tccatgcgtt gcttttcttc ttcggattca attgccaaag gcggaatggt tgtgggatgt
    16081 ccgccatcat ccaatgcgac ataaacgaga taggcggtgt tggtatgtgt gcgttcgcct
    16141 gagacggggt tttctgcgat cacctgtact tccgcttcca tggaggtgcg gcccgtatag
    16201 gtgacttctg cattgagaat gataaggtct ccgattttga tcggttcacg gaaaaccatc
    16261 gaatccacgg ctacggtcac gactttcttt tgggcatggc gcatacaagc cagtgcgcct
    16321 gcttcatcca ccagtttcat gatccagccg ccatgcacat tgccgagcag gttggcgtgt
    16381 tcggggtgca tcaattgcga gagggtcacc cgggagtggc gggcgctttt tggggtgaga
    16441 gtctctgtca tgggcagaat cagtccaggt cctggcgttg ttcgcctgca tccagcgtat
    16501 gcagacgttc aggggcgtca agcaaaatgt ccactgcgga tggtcccagg tcgggcatca
    16561 tccgcgcgag ggggacggtg gcgggcggaa aaaccttttc aggctttggg ggtggctgtt
    16621 ggcggggttt aagtgtggac gtggcccgtt tgccgatgat gcgcgggtcg ttggtcagtg
    16681 tgccgacgaa gtacccaagc acagaatata cctcgcgctt ctggttgacg aagccgatcc
    16741 aatcgccaag acgattgaac aggtacgggt agagcaggaa cgcttctgca tcgcctttgc
    16801 tggtgtaaat ggggataagg acgggttttt gtgttgacat ggtcaagcct cactggagtg
    16861 gtcaatatca ttatacgtcc agtaacggtt tacaaaaaag ttccagatca tcacaatgcc
    16921 cacagcagaa gccagggtga tgttttttgc atagaactct gctgtggcat atgaagcatg
    16981 aaaaactcct tcaaaaaaat ggaggagtgg cggttcaaga taatgcaggg ttgggatgcg
    17041 aatcaccacc cctgcaacat ttaccagaaa gaacatgcca agctggttga taagcggccg
    17101 tgaacgtgat tctggatagg tccaaaagcg gttccagatg aaattgctga cgaccgcaca
    17161 gatgaaggaa atcgtcccgg cgtatacgag ggacatgtcg gtgaagtggg agagcaaatt
    17221 catcacaccg aaatcgatca cagcgccgat cgttcccacc atcgcaaatt tcaagaagcg
    17281 tgtgcgttct tttgcgttgg tgaggatcat gcaagcccca gtttttgttt gaagtacgga
    17341 atggaacgaa tcagaccttc ctcaaggctg accttgggct cccagttcaa aatggtttgg
    17401 gcgcgggtga tatcggggcg gcggcgttgc gggtcgcgtg cgctgcgggc atctacaaaa
    17461 acgatgcctg cggtattgcc tgtcactttg ttgatggtct ctgcaaattg cagaatggtg
    17521 cgttcgtgcg ggttacccat gttgacgggg aaatgctcct ctgaataaag caattttacg
    17581 atgccatcga taaggtcatc tacaaaacag aaggagcggg tttgtgaacc atcgccatac
    17641 acagtgaggg gttggcccag caaggcttgt ttgatcatgt tcggcagggc gcggccatct
    17701 tcgagatcga gacgcgggcc gtaggtgttg aagatgcgta cgatgctggt gttgacgttg
    17761 tgagagcggt gataagccat ggtcagcgat tcggcgaagc gtttggcttc atcatacaca
    17821 gcgcgggtgc cgatggggtc cacatttcct gtgtacgatt ccacttgcgg gtgtacttca
    17881 gggtcaccat agatttcact ggtggatgcc agcaggaagc gggcattttg tgcgcgggcg
    17941 aggccgagac aattgtgagt gccaagcgca ccggccttca tggtttgaat gggcaggttg
    18001 aaatagcccg atttcgattg cggattggga gaggcagggg aggcaaaatg caagatcgca
    18061 tccactttac cggggacaaa gatgtattca gaaacatcac gacggtagaa ggaaaaatgg
    18121 tcgttatctg ccagatgggc aatgttatcc gggctgcctg tgacaaaatt gtccatgccg
    18181 atgacttcgt gtccatcttt tagaagatgg tcgcaaagat gagagccgag aaacccggct
    18241 gcgcctgtga tcaaaattcg cattagtttt atcctttgat ttaggtttat ttccaatcta
    18301 ttccaacgat caacccatca ccggtgacct gataggcttt tccgcttccg ctatcgatga
    18361 gccacaaatt gccttgatag gtgacggcaa taaaatcgcc aacctgccct tcgagtggtt
    18421 cgggcgccca cgacggtgtc tgcggttcaa tcccgttcgt gtcggcgggt gggaacaaag
    18481 ccctgctgtt cgaaccgtcg cggtccatta ccatcaaacg atagcggctg gtttcgcttt
    18541 gctcaatgaa aatggattgc aggtatgcca cctgataatc tttttgcctt ccatcccatc
    18601 tggatggcga cacagctgga taggaaaaca tgcccgccgc atctataagg gagacagacg
    18661 ctccattttc gaacgagatg gctgccacat caaaaaaagg tgactcctcg ccgacgatgg
    18721 gggcgggtgc cggcgcatgg gtgacatagt acagggtttt tccatccgaa ccccaggtga
    18781 tggggggaat ccaggcccaa tcgctgtggg tgttgagcgg ggtaattccc agcatcggtt
    18841 tcaggtaacc gccgtcctga tccaccagac cgatcccatc cgggcgggca taagcgaggc
    18901 ggccgtcttc cgaatatgca aacgacatgc cccaccaacc atacacgccg ccgctgtttg
    18961 cttccagcat cttgcgcgga cttccaccag tgagactgac tcggtacaag tcattgtttg
    19021 cctgccagcc gggggcggta ctacgcggct ccacagtgga gtatgcaacc gaatttgtgc
    19081 cgggaatcca ttcggcaaaa tgtactacgt tataagcctg cagccagatg ggttttggtt
    19141 caatgttttt cgtgcgaacc gcccatagag tattgatctc ctgactggcg ggctttttcg
    19201 atttacgagt gaagatcagg tattcgccat ttggcgataa tttgaagatg cgtccatcga
    19261 ggtcaccagt gctgacaatt atgcggcggt tggctgtgga gccttccatg atccatgcat
    19321 tgccacccgc aaggtacacc aacgtaccgg ggatgttcaa cggagtaaaa gatgactgtg
    19381 cgcctaccat tacgatctcg ggcagggctt ccgtcagtac cacggttttt accgaggtct
    19441 tactgatttc gacctgattt tcaagaatgc ggcgatacgt gacttccaat aagccgttga
    19501 cgccagcctg caccaaccgg gtttcccctt cgggcagggt ttcattgcgg acgatctgcc
    19561 gttcaaacgg aactgtgacc tgctcagtct cgaagacttc ttcgacccgt gtcagggtga
    19621 tgctatcgcc gttcgaaagc acagtgtaca aaggcggctc ggaacggtct aatttagcgg
    19681 ggatgatgcc tgcgctttgc aatgcctgtg acacggtgct gcctgccgtt acgctgacct
    19741 gggccgcatt tccgtcggca ttgatggtga ctgtgatctg ctcaccaacc tgtggggaac
    19801 ggcacgaccc tgtcagccag atggatatca atagaaacag gagcaggtgc gggcggagtc
    19861 tgaggcgggg catcgggtat aatccagccg ctatttgata cggccagtca ttgtcatcac
    19921 gccgtctgcg atggtgatcg cttccaaacg aaagcctgtg gcggctggcc ccaatgaacc
    19981 tgtgaaagcc tcagcgatca tggcattaat ggcggcgttt aacccttcgg gtgctgggat
    20041 ggggccaagg tctactgaag aaacttcgat tttaggcata cctgtggttt cgtcaatgcc
    20101 gacactggct gtgataagca tgttcgcgtt gaaggatccg ctggcgagtt ttccgtacat
    20161 ttgcagttga ccgttgcgaa gcaggacttg cgggtcggta aatggcgggt cggtttgctc
    20221 ttgaagtttg agcgcgataa gcgaagtgag ctggttttcg gtgatctgca aggtgacaac
    20281 cccggtttcg gcaccagccg taaaagcctg ctcgatttgg gtgtacatgg tttgtacttc
    20341 atctgccgag acgggtacgg tttgtgtggg gtagtctggc ccgccgacga agatattgca
    20401 ggccagtgag gccagcatca acacggcaaa caggaaagtt aagtggtaaa tttttgtttt
    20461 catggttttg atttttgatt tcaggatact aagcggagca attatagcat gagcgactct
    20521 caaactactt cggcctctgt ttcaactcca gagcaatcgc tggcggcaaa gatcgaggca
    20581 atgctgttcg tttcggcgga gccggtgccc atggcccagc tcgccgccgc gttggatgtg
    20641 actgcctctg tggtggagcg cggcctcaag gaattggatg aagcccttct aaaccgtggt
    20701 ttgcgcctgc agcggaatgg cggacgtgtg caactgacca cagccccgga aatggccgtg
    20761 ctgatcgaaa cattccttgg gcttgaagcg accacgcatc tcagccgcgc agccctggaa
    20821 acccttgcca tcattgccta tcaacagccg tgtacgcgcc cacaggtgga tgctgtccgt
    20881 ggtgtgaata gcgatggcat gttgaagagt ttactaagca aggggcttgt gcaggaagca
    20941 ggccgtacag acggccctgg ccgcccgatc ttgtattcca ccactccgga gtttttacag
    21001 catttcgggt tggggtccat tcttgaattg cccccgctct cggcccccac agagcctgat
    21061 aacgagcaca gtgagttgct aaaaggataa agtaaatttc aaaaacggga tgcaagagga
    21121 ttgcatcccg tttttttgtt ctgaactttt ctgaatatga tttatttcat actgtgaacc
    21181 tgccgcagca gccctgccac catttcttcg gcgggcatgg tgagcgattc cttttggaac
    21241 atcaatacac ttaccacggg catatccacc atcatgtcca tgatatccat gcccatcacg
    21301 gtctgttccg taacttgttc tcccgacttc gtattgccgt agcggtcttc cccggcaaat
    21361 aacctgcgaa cctggttttc gatctgctca taaaggctgc caagcacctg ccggccgcgt
    21421 cggtctgcca tccactctcg cagagtggat tcaatatcga gaatgcaggg caggtccaag
    21481 gtcgattgaa gcgtggttgt gaggcggctg cgaatgtcag cggaggaagc tccgatgagg
    21541 atgtcaaagc cgccatcttc ggtgatccac tgcttgtgtt cggggtggta gtaggcgaaa
    21601 gcacggaaat ccaaatggat ggatacggtt ttggtttcgc cgggttgaag ttccactttg
    21661 gcaaagccct ttaattcctt gacggggcgg atcagatcgg acttttggtc gtggacgtaa
    21721 acctgaaccg tttccttgcc tgcgaggttg cccgtgtttt ttacatctac tgtaacggtg
    21781 aggccatcta catctttgaa attgttcgaa gagactttgg cattgctata ttcaaaagtg
    21841 gtatagctca gaccatgacc aaatggaaag agtactggta tttcttttgc atcgtagtag
    21901 cgataaccaa tgaaaatacc ctcaccatag tgaaccttcc ccgctccgcc gggccagtta
    21961 atgtgcgacg gggtatcggt aagtttgagc gggaaggttt cagcgagttt gccgctgggg
    22021 ttcacacgcc caaaaagaat gtcggcaaga gccgcgccgc ctgcctgtcc catcatccag
    22081 ccttcaagca cagcagagac atcatcgatc cattcgctca tggcaacggg tgcgccgttg
    22141 ttcaagacca ccacagtttt gggttgggct ttggaaactg ctttgatcaa cgcgatctgt
    22201 tgcagggtca ggtccaggtc atggcggtcg tagccttcag attccttgaa ggtaggcagg
    22261 gcaatgtaga gtaatgctac gtcggctgat tgggcaagct tcacagcgtc atcgatcatg
    22321 ccttgttgga aggagttgtc agtggggtag ccttcggcat aggtcaactc ggcattttcg
    22381 gcacgcgcct gaagttcttt gtacggcatg gcaaccttgg tggggttgat gtgtgaactg
    22441 ccgccgccct ggaaatgtgc tgtttctgcc gaacgtccga tcactgcaat gtgctggtga
    22501 tctttcagtg gcaacaaccc atcattcttt aacaacacta cgccttctgt ggcgatcttg
    22561 cttgctaatt cgtggtgagc gtccacgtca aatgtgccgt ttcctttggc agtttctttg
    22621 gctttgaaca caatgcgcaa tatgcggcgt acagattcat ccaatgtggc ttcactgaga
    22681 tcacctgaac ggactgcttc caccacggct ttcacgcgta aatcctgcgg gccgggcatt
    22741 tcccaatcca aacctgcttt gagcgcggag acgcgatttc gtaccgcacc ccagtccgag
    22801 acgaccaggc cttcgaagcc ccattcttct ttcagaatct cggtcaagag ttgttggtgt
    22861 tcagacgcaa acgttccatt gaccttgttg tacgagcaca tcaccgtcca cggttgggct
    22921 tgtttgacgg ctttctcgaa ggcgggcaga tagatctcgc gcagggtgcg ttcgtcaatt
    22981 tcagcgctga tgctgaaacg ttggaattct tgattgttgg cggcataatg cttaagtgaa
    23041 gtgcccacac ctttggattg aataccattg ataaagttcg cggctaattc gcccgcaaga
    23101 aatgggtctt cggagaaata ttcaaagttg cgcccattaa gcggggagcg tttcatattc
    23161 acgccgggcc cgagcagcac atccacattg agggcgatac attcttcggc gagggcaatg
    23221 cccatttgct tgatcaggtc cacgtcccag gtggaagcgg tgcaggatgc tgttgggaag
    23281 caggtggcag gcaggctttt ctgtcccatg gaacggacat ctgctacacg tcgtacaccg
    23341 tgtggaccgt cggagacgat catctcagga atccccaggc gttcaacggg cgtggttgac
    23401 cagttgctgg ctccagtaca aatggccgcc ttttcttcga gcgtcatttt agaaatgatg
    23461 gatttaatat ctttagccat tgtttgtttt cctttttatg actcggtata ttcagaacgc
    23521 aatataaagc cgactaccaa atcgtattca ttggctggtg caagttcaaa gtcgggaatg
    23581 gcaccattca aggcaatcag tagatgcaaa gaagcggttt tgagccaaat ccctgccatt
    23641 aaatcgtcaa cgttgttttc agcaagtctt gatctcttga agaaggtccg acaaggattt
    23701 caaactcgcc aggctcaaca atatattcag ccttcgtgtt cacgatgcgg aaggactccc
    23761 aaggcagttc cacttcgacg gtctttttct cgccaggttg gagttgaacg cgggcaaagc
    23821 ctttgagcgc tttgttcacc caagtgactg aggtcactac gtcgctgacg taaacttgca
    23881 cgatctccac accttcacgt tgaccgatat tctccacgtc cactaaaacc ttcactgcat
    23941 caccttgcgc gagtgttgaa gaagacagac gcaggttgga gtaggtgtat tttgtgtaac
    24001 tgagtccatg cccaaatggg aagagcggtt cttgggtcag gtcagcgtag cggctgccat
    24061 gttgtccgcg gacttggcta tagaagatgg gttgctgccc gacatgcaca gggatgg
//
LOCUS       DAOQJC010000059        23863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_933_length_23863_cov_9.065356, whole genome shotgun sequence.
ACCESSION   DAOQJC010000059 DAOQJC010000000
VERSION     DAOQJC010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 23863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23863
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_933_length_23863_cov_9.065356"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1285)
                     /locus_tag="PLE39_10515"
     CDS             complement(104..1285)
                     /locus_tag="PLE39_10515"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNE68805.1"
                     /translation="MKYTDLKIQTQREAPNNARTEGFALLVRAGYMTRECEILPLGKE
                     ALTRLETLSNDPSFSEVVDLPRLVSEQAVFFPMVTGNLEVAHCPSCKYTELLDMAKFK
                     KTPLPHEEERPLEKVLTPDCNTIESLANFLGLPKEKTAKALMFTRKEDGKFVFAAVRG
                     DMQLSEAKLEYIVGEIQPANAEAISRSGAVAGYASPIGLNDALILVDDLIPQSQNLAA
                     GANEAGYHLLNTNYPRDYSAEIVEDLVMAKAGDACSNCGNALEVVSAMPLGTRFGLYY
                     ENWLLALAERHHDDKGLTFPKSAAPFDVYLMHVPGKQMDTRAEAEKIYNQLQNAGISV
                     LFDDRDERAGVKFNDADLIGCPIRVTVGEKALQNGMVELKPRKEKENKLVPLEKIIEE
                     L"
     gene            complement(1340..>1850)
                     /locus_tag="PLE39_10520"
     CDS             complement(1340..>1850)
                     /locus_tag="PLE39_10520"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNE68806.1"
                     /translation="EAVQPVQAVERITSVLGSGLIWHGSINGSGGVRIEGAFEGEIAL
                     RGMLVVGETGRVTCQNVRANTVIVAGAVRGNITTQKLEIRASGRVWGDVVTTAFVTEE
                     GAFLRGQIRMEETVDLDLEPAPESTPSEAAQAESIAQTPIQIPTPEPTLPTDSTQKLM
                     RNGKKKSEV"
     assembly_gap    1851..1950
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2071..2868)
                     /locus_tag="PLE39_10525"
     CDS             complement(2071..2868)
                     /locus_tag="PLE39_10525"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HNE68807.1"
                     /translation="MGLLEGKNALIFGLANDKSIAWGITQAFKREGANIGISYAGEML
                     ERRVKPLAEQVDCQWLEECDITKDDHIAQTVEKAAKHFGKIDVLVHSIAYAGRDELSK
                     PFHETSREGFKTALEISAFSLVALTHAFLPIMNPGASVMCMTYYGSVKVAPHYNVMGV
                     AKAALESSTRYLAYDLGPQKVRVNAISAGPIRTLAAAGVGGFRDMYKHFADMAPMREN
                     VTIEDVGNSAVFLASDLSAHITGEVLYVDSGFNIIGVQVSEKDEKGG"
     gene            complement(2904..3482)
                     /locus_tag="PLE39_10530"
     CDS             complement(2904..3482)
                     /locus_tag="PLE39_10530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNE68808.1"
                     /translation="MTLPRKFYNRPTLTVARELLGARLVRISDGMKLVGIISETEAYF
                     GFDDLASHAKAGRTIRTDPMFGPPGLAYVYFTYGNHWMLNAVTEKEGFPAAVLIRAIQ
                     PLEGVDVMMERRQGRDTLGPGKLTQALGITKSENYADLTESASSLRIEAGFKVPDKCV
                     TIGPRVGLNTTPEPWFSKPWRFLVKETIADIE"
     gene            complement(3545..4468)
                     /locus_tag="PLE39_10535"
     CDS             complement(3545..4468)
                     /locus_tag="PLE39_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE68809.1"
                     /translation="MNFLITGAAGFLGSSLANHLAREGHQVRGLDDLSTGDPKVLAPD
                     VHFTRGDVSDRPKLWTLLQDVDVVYHLAARVSVQESILYPRDYNAVNVGGTVALMEAM
                     RDVGVKRVVLASSGAVYGDLGDSTLTESTRPNPRSPYAVSKLAAEYYVNTIGSLWKIE
                     TVSLRIFNAYGPGQHLPPSHPPVVPHYLRQAQRGGTLVAHGDGSQSRDYIYVDDVVSA
                     MVAASTAPNINGAVINVGSGTETSVKDLIRIVLDVTDSKANVVYNPQTSGGVSRMKAD
                     LNLAKEKLRFTPSIKLEEGLRLTLQRDARFK"
     gene            4686..4898
                     /locus_tag="PLE39_10540"
     CDS             4686..4898
                     /locus_tag="PLE39_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNE68810.1"
                     /translation="MSDRIIGTVKWFNGDKGYGFLAREGGADVFVHFSAIQGDGYRNL
                     QEGQKVEFTVEKGPKGPQAANVTILA"
     gene            complement(5060..5410)
                     /locus_tag="PLE39_10545"
     CDS             complement(5060..5410)
                     /locus_tag="PLE39_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68811.1"
                     /translation="MPESEADRLKRLRERQLRDRDPLERERKFQRNSAIKEKRMRKPI
                     SLQEDWGKVSYTIKMPIYALLLGGIGTVILTKVWISPYAVYTGAGITIVLLIFGVLVG
                     NAVDLREDIKKHLK"
     gene            complement(5512..5964)
                     /locus_tag="PLE39_10550"
     CDS             complement(5512..5964)
                     /locus_tag="PLE39_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68812.1"
                     /translation="MKTYLLAWNPKRWAWKNLPVMAQQVKDGKMILDRWGTGSSQKLK
                     RGDRFFLIRLGENPRGIFASGEVMTDSFQDLHWDEQKALLGETANYVQIKYDILLDPD
                     HGPILPRELLVMPPLSGMHWEIRMSGIEIPAHIARELERLWAEFTEQL"
     gene            complement(6105..7355)
                     /locus_tag="PLE39_10555"
     CDS             complement(6105..7355)
                     /locus_tag="PLE39_10555"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE68813.1"
                     /translation="MDFNQFQLDSRLMQGIQKAGYEKATPIQEAAIPAALRGRDIIGT
                     AQTGTGKTAAFVLPILNKLLSGQRNTARALIVTPTRELAEQINDVIKVLSAGTKLKSA
                     TIYGGVGANPQIQALRNGAEILVACPGRLLDLIAQGHAKMANIEFLVLDEADRMFDMG
                     FLPDVRRIVKAVPEKRQTMLFSATFPSDVELLAQQALKQPQKIAMGIIKPAHTVTHAL
                     YPVAPHLKSTLLLELLKRTDTNSVLVFTRTKYRAQKVAQQIHKAGLKVTSLHGDRSQG
                     QRQAALKGFKSGHHDIMVATDIAARGLDIETISHVINYDMPDTADAYIHRIGRTGRAQ
                     RTGDAFTLVTHEDNDMILILERIMGQRIKREYLEGFDYTRPAPPKTDSGRGRGAPRDD
                     SRRPPRPAPTPKSYGRNRLAPKKK"
     gene            complement(7585..8481)
                     /locus_tag="PLE39_10560"
     CDS             complement(7585..8481)
                     /locus_tag="PLE39_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE68814.1"
                     /translation="MNFIGEIAGLATSFFFAITAIIFASVGRMVGSQVTNRFRLLFAL
                     FYLVILNLIFFHEPLPFSAGLPRWLWLSLSGVIGLSLGDAFLFRSYVAVGPRLGSLLL
                     SLAPIFGSVIAWIFFGETLTALQILGILLALAGIGWVVTSHKEEANTPKGHTREGIIF
                     GVLAGLGQAVGLVLSKQAMAGGFSPFQANAIRMLAAVIFTWAWTAVEGQISSTVETIR
                     NHPKALQWIAFGAVIGPVLGVSSSLLAVQHAEIGVASTLMALPPVIVLPISYFVFKEK
                     IGWQGIAGTFLAILGVAVLFLA"
     gene            complement(8485..9366)
                     /locus_tag="PLE39_10565"
     CDS             complement(8485..9366)
                     /locus_tag="PLE39_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE68815.1"
                     /translation="MRKLIGILLIAISAASFGTLAIFGRYAYENGMDTFTVLFLRFGI
                     SASFMTVILLLRKEHLPRGKILAQLIGMGALGYVGQSFMYMTAINYASAGLVALLLYL
                     YPFFVAVLSMIFLHESLTRVKIIALVLALFGTALTVGPVSGQIIGALMAITAALIYSI
                     YIIVGTNVMKHVTAVQSSLVIFASAGAVFGILAFSKGTHFPVNNMGWFAMLGIIVIST
                     IIPVVTFLAGLERIGPTNAAMLSTLEPIVTVLLAAWLFGDKLLPIVMLGGGLILVAVM
                     LLTRAELGKDSGNQPGV"
     gene            complement(9422..10018)
                     /locus_tag="PLE39_10570"
     CDS             complement(9422..10018)
                     /locus_tag="PLE39_10570"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HNE68816.1"
                     /translation="MEMIHTYQINGLTLQTGDIICTMNGKPDILPGEFWRLIGRLVPG
                     DVDHVAIYLGPDGRCAEAGARGVITFDVPNGHWDTERMARQRGLLFDTFYGIAAPLDV
                     LGFSDEDEQLMREAVADYVLAQLGKPYNLNFFNPETEETFYCSQLAYKAYQKIGIDLN
                     TGLAMEQLPGTNQIIYPQEIWDGFSHRQSEARQETISR"
     gene            complement(10059..11108)
                     /locus_tag="PLE39_10575"
     CDS             complement(10059..11108)
                     /locus_tag="PLE39_10575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE68817.1"
                     /translation="MKIGLVIYGSIDTLSGGYMYDRMLVEYLRAQGDSVEIISLPWRN
                     YAAHLTDNLTFKLPPNLDILIQDELNHPSLIGANHGRHPYPIISLVHHLRCSELRPKW
                     QNDLYRPIEKRYLQSVDGFIFNSKTTKGVVNGLIGSEKPDVVAYPPTDRFGDAISEEE
                     IKERSKKKELHMLFLGNVMGRKGLHTLLEAVRLSNLDLRLDIVGSLTTEPEYAAQMQE
                     FVSTNGLSSAVHFHGALNNQPLIERLKAAHILVVPSSYEGFGIVYLEGMGFGLPAIGT
                     TLGAAGEVIEEGVTGYLIQPGDSHALAARLQLMNEKRDLLIQMSLAARNRYLAQPKWN
                     QTAHQIREFLLSFSG"
     gene            complement(11160..11546)
                     /locus_tag="PLE39_10580"
     CDS             complement(11160..11546)
                     /locus_tag="PLE39_10580"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNE68818.1"
                     /translation="MYTLGVRREFIARHFLIGGDWGPENFPNSHHYILELQLKGSELD
                     QHGYLVDIVDVEKHLDDVVGYYKEQMLNEKPEFAGLNPSIEHFSRILAETLNERIKAR
                     NISKLKVVLWEHENAWAAFSVERLAG"
     gene            complement(11574..12260)
                     /locus_tag="PLE39_10585"
     CDS             complement(11574..12260)
                     /locus_tag="PLE39_10585"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNE68819.1"
                     /translation="MKFNYTTLETCHTATGIVLVIDVLRAFSTAAYAFSRGAREIRLV
                     SAVEEALELKAQLPNSKVMGEVGGLPPAGFDFGNSPTRILEHDLTGITLIQRTGAGTQ
                     GAVHSVHAEVMFAASFVVAQATMEHVLRLRPEEVTFVVTGGADNAEDLACAEFFEKQF
                     TGQAVDSEELIRRVVQSRDAFQHMNDHPQFPRSDLDLCTRINAFNFAMPIERKNGFLI
                     MQALNLKPEN"
     gene            complement(12257..13267)
                     /locus_tag="PLE39_10590"
     CDS             complement(12257..13267)
                     /locus_tag="PLE39_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HNE68820.1"
                     /translation="MPKAQTIFFTAPKQVEIKEITLPPLKKDEVLVETICSAISAGTE
                     MLVYRGQFPHLADSHDTFSSDLNYPLAYGYACVGKVKEIGADVSQEWKDKHVFAFHPH
                     ASSFIINPSSLIPIPASLTSENACFLPNMETAVNLVQDAAPILGERVLVLGQGVVGLL
                     TSSLLSEFPLESLLTADFYELRRKAAMQIKGVHSLDSSLKTFHTDSVAYTQNKYDLTL
                     ELSGSPSALNDAIELTIFSGRIVIGSWYGQKRTEIDFGGSFHRSRIRLISSQVSTISP
                     ELSGRWDKSRRFDVAWQALEQIRPEKWITQRFSIADAAKAYQLLDEKPHETIQVVFDY
                     LK"
     gene            complement(13330..13716)
                     /locus_tag="PLE39_10595"
     CDS             complement(13330..13716)
                     /locus_tag="PLE39_10595"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE68821.1"
                     /translation="MQERRKQQRKNLMAYTQVFDLNGGHLLGYLGDLNLHGAMVIGEK
                     PIAENTELTLAIELPELENVKIPRITIPARAAWCQQDISPEYFNVGFEFKEVTPKQKP
                     VIEEIMEKYEFRRDLPSYLTKPVPNK"
     gene            13804..14529
                     /locus_tag="PLE39_10600"
     CDS             13804..14529
                     /locus_tag="PLE39_10600"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNE68822.1"
                     /translation="MRLIYISPHLDDAILSAGGLIHDQVQAGRHVEIWTVFCGFPPAN
                     QLSSFAQIIHQKWGIMDTNELVAARRAEDKAAADIVGAQVFHFDFLDCIYRRGRDGNW
                     LYSDVFIPPHTDDDGLPAQIAEAISARLNPDDELVCQLALGAHLDHTLVRRAAELLGR
                     PLTYDVDIPYLFNFPDDFALKTAKMKEITHRITEAGLRSWGDAILAYTSQFGTLFSSP
                     EEMREKLQQYWAEKQGISFWKFE"
     gene            14670..15146
                     /gene="greA"
                     /locus_tag="PLE39_10605"
     CDS             14670..15146
                     /gene="greA"
                     /locus_tag="PLE39_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNE68823.1"
                     /translation="MPTTNFLTKEGFQKLQEELDHLRTVKRQEVADRLHEAMEGGELI
                     ENAEYEAAKNEQAFVEGRIQELEILLATANIIEENNGKAKKNDLIHVGSKVTIKEGNF
                     EAETFTIVGAAEANPREGKISNESPIGKAILNHKLGDIVKVETPGGTYNVKIIKVG"
     gene            15249..16781
                     /gene="lysS"
                     /locus_tag="PLE39_10610"
     CDS             15249..16781
                     /gene="lysS"
                     /locus_tag="PLE39_10610"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNE68824.1"
                     /translation="MAEYNSLEKIRLQKIEELRAEGIESYPTRAERTHTNAKVIAEFE
                     AAEKNAAPDTTPEVKAVLVGRIRANRAMGKVSFAHIEDGSGKIQLFFRSNELGKERLD
                     LFNKMFDLGDFIQASGVMFRTKTGEVSLHVHDFKLLAKSVTPLPADKDVTQEDGTVTR
                     YAALESPELRARQRYADLAVNPDVRETFRKRATLIKALRNFLDGHDFLEVETPILQPL
                     YGGAAARPFVTHHNELDQDMYLRISFELYLKRLLVGNLERVYEIGRDFRNEGVSFKHN
                     PEFTQLEFYWAYADYLQVMELTEQMVAYVAEQVTGSTKVTFGEYELEFKPPWNRVEMR
                     QGILETSGIDIAEHKTAEELFKAIRAKHPNKTPDPKSARGKMIDFLLGEFLEPTLIQP
                     TFLYNYPRDISPLAKSIPGDPLTVERFEGYAAGFELCNAFTELNDPLDQEQRFIEMGR
                     DYTDDEEEKHPLDEDYLRAMRYGMPPNGGFGMGVDRLAMLLTNKHSIREVLLFPALRK
                     EE"
     gene            16789..17448
                     /locus_tag="PLE39_10615"
     CDS             16789..17448
                     /locus_tag="PLE39_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68825.1"
                     /translation="MHYISPDKEEQIRQIARSGNLIEAIKEYRLLTNASLKDAKSAVE
                     AMVRGNYVNGPAPQAVANGADSSQKSQILDLMSKGREIEAIKLYRGWTKAGLKEAKDA
                     VEAMARGEDVQIPEPVTSVESGSMDGQIRALLAKNQKIEAIKVHRAATNSSLKEAKDY
                     VEAIERGVRPNPSSPLNIPLSNDPFEEENVRTRRMMVFVFIILAILAVVFAFAFLRGG
                     L"
     gene            17457..17843
                     /locus_tag="PLE39_10620"
     CDS             17457..17843
                     /locus_tag="PLE39_10620"
                     /inference="COORDINATES: protein motif:HMM:NF022700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HNE68826.1"
                     /translation="MQKSKLVLIFGDVIAIALLTFIGFATHGEADVSFLPRMAAAFFP
                     VLIGWFVLAPWFGLFDEQIISNPRSLWRILPAMLAVAPLAVMLRAAILNAAALPLFTL
                     VLGSTNAFGMLVWRALHLFVVKRNAR"
     gene            17888..18496
                     /locus_tag="PLE39_10625"
     CDS             17888..18496
                     /locus_tag="PLE39_10625"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE68827.1"
                     /translation="MDEPALIQTAQNGDLDSFNTLILHYQDRVYNTALRILGDEDQAA
                     DAAQEAFISAFRSINSFRGGSFKAWLMRTVTNACYDELRRQKRRPTTPLEPDTADGEE
                     MDSPRWLADPNMTPAEKSEADELEHAIQHCLDALPTDFRTVVVMADLQGLDYTEVAAA
                     VRVPLGTIKSRLARARLRLRECLRGFEELLPSSFRLEEESRL"
     gene            18493..19278
                     /locus_tag="PLE39_10630"
     CDS             18493..19278
                     /locus_tag="PLE39_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68828.1"
                     /translation="MKNFRDIEQLSSYLDGQLSPSDSARVESRIASDPELASAFEDLR
                     ATRGILRKLPARKAPRNFTLTRQMVGLKPPVSRSYSFFRFSSAFATVLLMLTFAANYV
                     APRMSVVPVGGSGGGCDPCGLGGGGGPESFNMDALPAATEAPAVAAESFLATEAPAAT
                     EAPAMVEEPAATEAPVEVLPTMDMALAPTELPLSTQDTQRIVETPSASPKEPVIESAP
                     QQQPPAEDESFISATWQVVLLVIGLVSGLAAFFMNRSAKKKWS"
     gene            19282..19767
                     /locus_tag="PLE39_10635"
     CDS             19282..19767
                     /locus_tag="PLE39_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE68829.1"
                     /translation="MARDSHIKFCPRCGAEVAHEEKFGMVRPICPQCGWIYFIDPKVA
                     AAILVEQEGRVLLVRRVNEPFRGLWTLPAGFVNGGEDPAKAAARECLEETGLHVHVTG
                     VLDVIAGREHERGADFIIVYHAEVTGGELVPADDADAAEWFLRSELPPLAFNATKKIL
                     E"
     gene            complement(19951..22110)
                     /locus_tag="PLE39_10640"
     CDS             complement(19951..22110)
                     /locus_tag="PLE39_10640"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNE68830.1"
                     /translation="MGNTTSKIGIPVIIAAVVICIACVCCAGVLLYIYGDSIFGGTSV
                     TTPPVVSTDPIIPADTTNLPEWTIIYYSDADDDILEGDLWFDINEMELVGSNTQMNIV
                     VQIDRAEGSFDGDGDWTDARRLLITQDSDLNQINSPVVQNLGEVDMGNPQTLVDFMTW
                     TIQNYPAKKYALIMSDHGGGWTGGFSDLQSGSSLSMPQIVQAIEVVQQQMGGQKFEII
                     GFDACLMGMVEVYGSLYPYSNYMIASEEVIPSTGWSYAAWMGQLAQNPSMDGRGVSQA
                     IISTYITQDTLLTLRSTSSSEIEEVEAATTLSAVESARIPDVIGAMNQFISVMATIDQ
                     RYVAQAREYTRSYYSIFGEDTPSPFIDLGNFSEILANATGDPAIFQASEQLKAAISSA
                     VVAEKHGQRMSGSNGISFHFPISDIYAYTEYNSDFPPYYAESAARFLQLSTWDEFLAY
                     HYTGQEFTPQEGQAYTPPRTNEINAPGASPLTIAPIQLSDTVISADETLTLSTTVSGD
                     VSYIYFVLFFYNQDANVTWLADMSFVSAPNTISVDGVNAPDYGESPIEISYEWEPYLF
                     VLKDGTNQAYALFEPDEYESADGVTTYSVYGQYTPVNATTPTDAKLTFDLDGNLLNIY
                     ALPDSDSNGIDTAVAISPQIGDRFTDYVQYYTFDANGVATYTNELSDDVFTYGEQGFW
                     FESYIPVDGQYSIGFYAVDFDNNITSSYEYITYQNAP"
     gene            22280..22954
                     /locus_tag="PLE39_10645"
     CDS             22280..22954
                     /locus_tag="PLE39_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68831.1"
                     /translation="MKKQYRMLFAFAALLLLVSIACNGGAAPTAAPVPTLPPAPTSQP
                     QSNNPPSNNNPIVPQPPALNEPPPSNGNSSDLVVFTDENNLLSFEVPGDWYYEQQTYE
                     HAYIDRITSTDGSAIIDSLVYNDGQAFTGNDNGRFALQLLHQYYSNTGKEGDISISGD
                     EMQPDGSEKLTWTSKSGGYSGVSFFELRGGDRMTFLMFTLYYANDADQAVIDILDHAI
                     TSYTIP"
     gene            complement(23023..23787)
                     /locus_tag="PLE39_10650"
     CDS             complement(23023..23787)
                     /locus_tag="PLE39_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68832.1"
                     /translation="MKTSKNLSSVLAISSLILAMLACNFGKADATPTPLPPTNPPPTA
                     IPPTEAPPVVEASPTPVEVVPSVDTSAFETQLQEFADEGYVSTTEGEITPIDPFEEEW
                     AQLNYYQWWTIDKEASDLVFSGHFKWSSSNSTPDLSGCGVVFGLQEGSDDHYVVFLDK
                     GRIAFFMSRGSSVYEVGKTRGPGRVNFGNPAEADFSLAVRGKSAYVSVDGEVTEYTLS
                     ADQGTNGQFALTLLSGTNSGYGTRCEMTDMFLFIPE"
BASE COUNT         5510 a         5866 c         6411 g         5976 t
ORIGIN      
        1 cgatgacagc ctgacggtcg gcatcggtca gatcggggga tgacatgggg attttgtatt
       61 gggtcatgtt tttttatttg aagggtggtg ccatgcaccg cctctacaat tcctctatga
      121 ttttttcaag ggggacgagt ttattttctt tttccttgcg cggcttcaat tctaccatcc
      181 cattttgaag cgccttttcc ccaaccgtca cgcggatcgg gcagccgatc aaatctgcat
      241 cattgaattt tacgcctgca cgctcgtcgc gatcgtcgaa tagaactgag atccccgcat
      301 tttgcaattg gttgtagatc ttttcggctt cggcgcgggt atccatttgc tttccgggta
      361 catgcatgag atacacatca aacggcgcag cagatttggg aaaggtcaac cccttgtcat
      421 catgatgccg ctcggcgagg gccagcagcc agttttcata gtaaaggccg aagcgcgttc
      481 ccaacggcat tgcagaaaca acttcaagtg catttccgca atttgagcag gcgtcgcctg
      541 cttttgccat taccaagtct tcaacgatct ccgccgaata atcacgcgga taatttgtgt
      601 tcaaaagatg atatcccgct tcattcgcgc ctgccgccag attttgtgat tggggaatca
      661 ggtcatcaac caggatgaga gcatcgttga ggccaatggg cgatgcatac cccgccaccg
      721 cacctgacct tgaaattgct tcggcgttag caggctgaat ctccccgacg atatattcca
      781 gtttggcctc actcagttgc atatctccgc gtacggcggc aaagacaaat tttccatctt
      841 ccttgcgggt gaacatcaat gccttggcgg tcttctcctt cggcaagcca aggaaatttg
      901 ccagcgactc gatggtgttg caatcaggcg tcaacacctt ttcaagcgga cgttcctctt
      961 cgtggggcag gggtgtcttc ttgaattttg ccatgtccaa cagttcggtg tatttgcagg
     1021 aaggacaatg ggcaacttcg agattgcccg tgaccatcgg aaagaaaacc gcctgctcgc
     1081 tgaccagccg cggcaggtca accacctctg aaaatgaagg gtcgttcgag agggtttcca
     1141 gccgtgtcag cgcttctttg ccaagcggca ggatctcaca ttcacgggtc atatatcccg
     1201 cgcggacaag gagtgcgaat ccctccgtcc gtgcgttgtt gggggcttca cgctgggttt
     1261 ggattttcag gtcggtatat ttcatgggat taacgaggga gggacacggc atggccgtgt
     1321 ccctatcact cgggtgaatt tagacttcgc tttttttctt cccgttgcgc atcaactttt
     1381 gggttgaatc tgtcgggaga gtgggctcgg gtgtgggaat ttgaattggc gtttgggcga
     1441 tggattctgc ctgggcggcc tctgaaggcg tggattcggg agcaggctca aggtccagat
     1501 cgaccgtttc ttccatccga atttgcccgc gcaggaaggc tccttcttcg gtcacgaaag
     1561 cggttgtgac cacatctccc catacccgac ccgaagcgcg gatctcaagt ttttgagtgg
     1621 tgatgttgcc gcgcacagcc cccgctacga tgacggtatt tgcgcgcaca ttctggcagg
     1681 tcacacgccc tgtttcgccc acgaccaaca tgccacgcag ggcgatttcg ccttcaaacg
     1741 cgccttcgat acgtacaccg cctgaaccgt tgatgcttcc gtgccagatc aggcctgagc
     1801 cgagcacgga ggtgattctt tcaacagctt gcacaggttg gacggcttca nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caattttacc ttgaaaacct taagcgcgga
     1981 gcaggggtag gggtggagca aacaggcaaa aaaaggcacg cagaccttcc tgtccgcgtg
     2041 cctgcttgct tatgcatgta ttgcttgctt ctacccgcct ttttcatcct tttcactgac
     2101 ctgaacacca atgatattga acccggagtc cacgtacaat acttcaccgg tgatgtgtgc
     2161 agaaaggtca gaagcaagga aaacggccga gttccccacg tcttcaatgg tgacgttctc
     2221 gcgcatggga gccatatcgg caaaatgctt gtacatatcg cggaagccgc ccacacctgc
     2281 cgccgccaat gtgcggatgg gtcctgccga gatcgcattg acgcggacct tctggggccc
     2341 gaggtcataa gccagatagc gggtggatga ttccagtgct gccttggcga cacccatcac
     2401 attgtagtgc ggagcaacct tcaccgagcc gtaataggtc atgcacatga ccgaagcgcc
     2461 cgggttcatg atcggcagaa aggcatgggt caatgccaca agtgaaaagg cactgatctc
     2521 caatgcggtt ttgaagccct cgcggctggt ttcgtggaaa ggcttgctca attcatcacg
     2581 gccagcatag gcaatggaat gaaccagcac gtcgatcttg ccgaaatgtt tcgcggcttt
     2641 ttcaacggtt tgagcgatat ggtcatcttt tgtaatatcg cattcctcaa gccattgaca
     2701 atccacctgc tcggccagcg gcttgacacg gcgttcaagc atttcaccag cgtagctgat
     2761 accgatgttc gcgccctcgc gtttaaaagc ctgtgtgatg ccccacgcaa tggatttgtc
     2821 gttcgcaaga ccaaaaataa gtgcgttctt tccttccagt aatcccatat caatctcctc
     2881 gatattcgat attgtatacc cgactattca atgtcggcta tcgtttcttt gaccaaaaac
     2941 cgccatggct tggagaacca aggctctggc gtagtattta aacccacgcg ggggccaatc
     3001 gtcacgcatt tatcggggac tttaaacccc gcctcgatcc ttaaactgga agctgattct
     3061 gtcagatcgg catagttctc acttttggtg atccccagcg cctgagtcag tttgcctggc
     3121 ccaagcgtat cccgcccctg acgacgctcc atcatcacgt caacgccttc cagcggttgt
     3181 atcgcgcgga tgagcaccgc cgcaggaaag ccttcctttt cagtgaccgc gttcaacatc
     3241 cagtgattgc cgtaggtgaa gtaaacgtag gccagccccg gcggaccaaa catcgggtcg
     3301 gttcggatgg tgcgtcctgc cttggcgtga ctggcaagat cgtcaaatcc aaaataggcc
     3361 tcggtctcgc tgatgatgcc aacaagtttc atcccatccg agattcgcac cagacgtgcc
     3421 ccaagcagtt cgcgggcaac ggtcagtgtg ggtcggttgt agaatttacg agggagggtc
     3481 attgttaatg tttgcaggtt ggaagattta caagttagaa ttttcaaacc ttccaacaag
     3541 aattttattt aaatcttgca tctcgctgaa gcgtgagccg caatccttct tcgagtttga
     3601 tggaaggggt aaaacgcaat ttttcctttg cgaggttcaa atctgctttc atgcgtgaga
     3661 cgccgccaga agtttgcggg ttatagacca cattggcttt gctgtcggtc acatccaata
     3721 cgatgcggat gagatccttg accgaggttt cggtgcccga accaacatta ataaccgctc
     3781 cattgatgtt cggcgcggtg gatgcggcaa ccatggcgct gaccacatca tccacataaa
     3841 tataatcacg gctctgactg ccatctccat gggccaccaa tgtccctccg cgctgtgcct
     3901 ggcgaaggta atgcggcacc acaggggggt gggagggtgg caggtgttgt ccggggccgt
     3961 aggcattgaa gatgcgcagg ctgaccgttt cgatcttcca cagagaaccg atggtgttga
     4021 cgtaatattc cgccgcaagt ttggaaacgg catagggtga acgcgggttg ggtctggtgg
     4081 actctgtcag tgtagagtct cccaagtcgc cgtaaacagc accggacgaa gccagaacga
     4141 ctcgtttgac tcccacatcg cgcatggctt ccatcaaagc cacagtcccg cccacattga
     4201 cggcgttata gtcgcgtgga tataaaatgg attcctggac tgagacacgc gccgcaaggt
     4261 gatagaccac atccacatcc tggagcaggg tccacaactt ggggcggtcg ctgacatctc
     4321 cgcgtgtaaa atggacatcc ggtgcaagca ccttggggtc acccgtggaa aggtcatcaa
     4381 ggccgcgtac ctggtgtcct tcgcgggcaa gatggtttgc aagagaagag ccgaggaatc
     4441 ccgcggctcc ggtgatcaga aaattcatag gaaaagatta taaccgcaat attggtgcct
     4501 gtttttgatt gactcgtgtt tggtactgtg ttatctttac tgccgatagg aaaacggtaa
     4561 gttacgttgg tttggagaac atcaacagct cacggtttga ccgtgggctg ttttgttttg
     4621 acctccgatg gaacaaaaac ggcttgacct aatacaaatt ttttagtttg ccaaaggagg
     4681 caaaaatgtc tgatcgaata atcggtactg taaagtggtt caatggtgat aagggctatg
     4741 gtttcctcgc ccgtgaaggt ggtgcggacg tgttcgtcca tttctccgcc attcagggtg
     4801 atggttatcg caaccttcag gaaggccaga aagttgaatt caccgtcgag aagggcccca
     4861 agggtcctca ggcggcgaac gtcaccattc ttgcataaat cataaaaagc ggacgatgtg
     4921 aaagcatcgt ccgctttttg attccaattt gtttatgctt gaaagacggg ggaatatccg
     4981 cttttttgtg ctttcatatc gtacaacagt atatcggcgc gttccgggtc tataagctga
     5041 aacgtctgct gcttttgtgt tatttcagat gcttcttaat atcctcgcgc aggtccacgg
     5101 cgttgccgac aagtacgcca aaaataagca ggacaatggt tatgccagcg cctgtataga
     5161 ctgcatatgg cgagatccaa acttttgtga gaatgaccgt accgattccg ccaagtaaca
     5221 atgcatagat cggcattttg atcgtgtaag aaaccttgcc ccaatcttct tgaagagaga
     5281 tcggcttccg catccgcttt tctttgattg cggaattgcg ctgaaatttg cgttcgcgtt
     5341 ccagcggatc acggtctctg agttgcctct ctcgcagtct tttgagtctg tcggcttctg
     5401 attctggcat gggcgttttc ctttcactat tcagcagagc ctgattcgaa aagatacttc
     5461 aattcatatt atatggcggt gatgcgtttg taagattaaa tttccaccct gctaaagttg
     5521 ttcggtaaac tctgcccaca gcctttccaa ctcacgggcg atgtgtgcag ggatttcgat
     5581 gcctgacatg cgaatctccc agtgcatacc tgaaagcggc ggcatgacca gtaactcgcg
     5641 cggcaaaatc ggaccgtgat cagggtccaa caaaatatca tatttgatct ggacgtagtt
     5701 tgctgtttcg cccaacaagg ctttttgctc atcccaatgc aggtcttgaa aagaatccgt
     5761 catcacctca cctgaagcga agattcccct tgggttctca cccagacgga tcaaaaagaa
     5821 tctgtctcct cttttcagtt tctgcgatga ccccgtgccc cagcgatcca gaatcatttt
     5881 cccatctttg acctgttgcg ccattacagg caggtttttc catgcccagc gttttggatt
     5941 ccatgcaagt agatatgttt tcatagatct tttagcagac acccgcctca tatgaggcgg
     6001 gtgtccattc attgatgatc gatttacttc ttttccgaaa attgctcctg ccgccgtcct
     6061 cggcgacggg gacgccgcct tgagcagaat atttggatag gttcttactt ctttttgggt
     6121 gcgaggcggt ttctgccgta gctttttggg gtgggggcgg ggcgcggcgg acggcgggag
     6181 tcgtcgcggg gggctcctct tcctcggcca gaatcagttt tcggcggggc aggacgcgtg
     6241 taatcgaatc cttcgagata ttcgcgtttg atgcgttgtc ccatgattcg ttcgagaatt
     6301 agaatcatgt cgttgtcttc atgggtgacg agggtgaagg catcgcctgt gcgttgggcg
     6361 cggcctgtgc gaccgatgcg gtggatgtag gcgtcagcgg tgtcgggcat gtcgtagttg
     6421 atgacgtgcg aaatggtttc gatatccagc ccgcgtgcag cgatatcggt ggcgaccatg
     6481 atgtcgtgat gaccgctctt gaagcctttg agcgctgcct gccgttgacc ttgtgacctg
     6541 tcaccgtgca agcttgtgac cttgagaccc gctttatgaa tttgttgtgc cactttttgc
     6601 gcgcggtatt tggtgcgcgt gaacaccaaa acggaattcg tgtcggtgcg tttgaggagt
     6661 tccagcaaaa gtgtggattt caaatgcggc gcaacgggat acaaagcgtg tgtaaccgtg
     6721 tgtgcaggct tgatgatgcc cattgcaatt ttttgcggtt gtttcaatgc ctgttgtgcg
     6781 agcagttcca cgtcggatgg aaaggtggca gagaacaaca tggtctgtcg tttttcgggt
     6841 acggctttta caatgcggcg cacatcgggc aggaagccca tgtcgaacat tcgatcggct
     6901 tcatcaagca cgaggaattc gatgttcgcc atttttgcat gaccctgtgc aatgagatca
     6961 agcagacgcc ccgggcaggc aacgaggatc tctgcgccgt tgcgcaatgc ctgaatttgc
     7021 ggattcgctc cgactccgcc gtaaatggtt gcgctcttca actttgtccc cgctgagagg
     7081 actttgatga catcgttgat ctgttctgcc aactcacggg tgggggtgac gatcagtgcg
     7141 cgcgctgtgt tgcgctgccc tgaaagcagt ttgttgagaa tgggcagcac aaaggcggcg
     7201 gttttgcctg tgcctgtttg tgctgtgccg atgatgtccc gtccgcgcag ggcggcgggg
     7261 atggcggctt cttgaatggg cgtggctttt tcgtagcccg ctttttggat tccctgcatg
     7321 aggcgggaat cgagttgaaa ttgattaaag tccatatctt tccttatttt attaaaacca
     7381 caaagcacat gacccattag ggccttgagt tcctggggag ttgggtgagc gcgccgcacc
     7441 taatttgatg agaaaaggct gctcatcaaa aagaatccgc ccgtagggtg cgacgcagga
     7501 ttcgcgaaaa tagcaagttt gctatcggtt cattatatcc ttgatattgt gttttgggct
     7561 tgtttaagcc tgaatgcaat gatcttacgc taaaaataac accgctacgc cgaggatggc
     7621 gagaaatgtc cctgcgatgc cctgccagcc gatcttttcc ttgaaaacaa aataactgat
     7681 cggcagcacg atcacaggcg gaagcgccat cagcgtactg gcaacgccga tctctgcatg
     7741 ttgcacagcc aataatgaag aagagacccc caacacaggg ccgatgaccg cgccgaaggc
     7801 gatccattgc agggctttgg gatgattgcg aatcgtttcg accgttgagc tgatctgccc
     7861 ttcgacggct gtccatgccc atgtgaaaat gaccgccgcc aacatgcgaa tggcattggc
     7921 ctgaaatgga gaaaatcccc cagccatggc ctgcttcgaa aggacaagcc ccacggcctg
     7981 tcccagccct gccagcacgc caaagatgat tccctcgcga gtgtgtcctt tgggagtatt
     8041 ggcttcttct ttgtgcgagg tcaccaccca gccgatgcca gccagcgcaa gcagaatgcc
     8101 gaggatctgc aaagcggtca gcgtttcacc gaaaaatatc catgcgatga ccgaaccgaa
     8161 aatcggcgca aggctcaaga gcaggcttcc caaacgcgga ccgactgcca catacgacct
     8221 gaacaaaaag gcatcgccca atgaaagtcc gatcactcct gaaagactca accacaacca
     8281 gcgcggcaag ccagccgaga aaggcagtgg ttcatggaaa aagatcaagt tgaggatgac
     8341 aagatagaac agcgcaaaca gaagccgaaa gcgattggtg acttgtgaac caaccatgcg
     8401 cccgacggat gcaaaaatga tcgctgtgat ggcaaagaaa aatgaggttg ccagtcctgc
     8461 gatttcaccg atgaaattca ttttttatac acctggttga ttgccgctat ctttcccgag
     8521 ttcggccctg gtcaacagca tgacggccac tagaatcaat ccgccgccga gcatgacgat
     8581 ggggagcagt ttatcgccga acaaccatgc agccagcaaa accgtaacaa tgggttcaag
     8641 cgtggaaagc atcgcggcat tggtgggacc aatgcgctcc agcccagcca aaaatgtgac
     8701 cactggaatg atggtggaga tgacgataat tcccagcatg gcgaaccagc ccatgttgtt
     8761 cactgggaaa tgcgttcctt ttgaaaaggc cagtatccca aagactgcgc ccgccgaagc
     8821 gaagatcacc agcgaagatt gcaccgcagt gacatgtttc atcacatttg tgccaacgat
     8881 gatgtaaatg gaatagatca acgctgcggt gatcgccatc aatgcgccga tgatctgtcc
     8941 gctgacgggg ccgacggtga gggccgtgcc aaagagtgcc agcaccagcg cgatgatttt
     9001 tacgcgggtc agggactcgt gcagaaagat catcgaaagc acagcaacaa aaaaagggta
     9061 gagatagagg agcaaggcca ccaaacccgc cgaagcgtag ttgatggcgg tcatgtacat
     9121 gaaagattgc cccacataac cgagcgcgcc catgccgatc agttgggcga ggatctttcc
     9181 gcgtggaaga tgttccttgc gcagaagcag gatcacggtc atgaaagagg cggagattcc
     9241 aaaacgcagg aaaaggacag tgaaggtatc catcccgttt tcgtaggcgt atctgccgaa
     9301 gatggcgagg gtcccgaacg aagcagcgga gatggcgatg aggaggatgc cgatgagttt
     9361 tctcatggtg ggaaatatac cgcactcggt ctcaacaata aatccctccg cgtggaggga
     9421 tttatcttga aatcgtttcc tgccgtgctt cgctttgcct gtgagaaaaa ccgtcccaaa
     9481 tttcctgcgg ataaatgatc tggtttgtgc ctggcagttg ttccatggcg agacctgtgt
     9541 tcaggtcaat gcctattttt tggtaggcct tgtaggccaa ttgactgcaa taaaaagttt
     9601 cttcagtttc agggttgaag aaattcaggt tgtaaggttt gccgagttgg gcaagtacat
     9661 agtctgcaac ggcttcgcgc atgagttgtt catcttcgtc ggagaagccg agtacatcaa
     9721 gcggcgcggc aatgccataa aacgtatcga acaaaagtcc gcgctggcgg gccatgcgtt
     9781 cggtatccca atgtccattg ggcacatcaa aagtgatcac gccgcgcgcg cccgcttcgg
     9841 cacagcgacc atcagggcca agataaatgg caacatggtc tacatcgcct ggtacaagcc
     9901 gcccgatcag ccgccagaat tcgcctggca gaatatcggg cttgccgttc atcgtgcaga
     9961 tgatatcgcc tgtttgtaaa gtcaatccgt tgatctggta agtgtgtatc atttccatgt
    10021 tcattatacg gaaattccct cccaaacgtt tcagccattc atcctgaaaa cgaaagcaaa
    10081 aattcacgga tctgatgcgc ggtctgattc catttgggct gcgccagata acgatttcgc
    10141 gccgcaagac tcatttggat gaggagatcg cgcttctcgt tcataagctg tagcctggct
    10201 gccaacgcgt gagagtctcc tggctgaatc agataccccg tcacaccctc ttcgatgact
    10261 tcgcctgccg cgcccaatgt ggtgccgatg gcaggcaggc caaaccccat cccttcgagg
    10321 tagacgatgc caaagccttc ataacttgag ggaacaacga gaatatgagc ggctttgagc
    10381 ctttcgatca gcggttggtt gttgagagcg ccatgaaaat gaacagcaga agataggccg
    10441 ttggtcgaga cgaactcctg catttgggcg gcgtattcgg gttcggtggt gagtgaacca
    10501 acgatgtcaa gtcgaaggtc aaggttcgaa agtcggactg cttcgagaag agtgtgaagc
    10561 ccttttctcc ccatcacatt tccgaggaag agcatatgca attctttctt cttacttctt
    10621 tcctttattt cctcttccga aattgcatcc ccaaacctgt ctgtgggcgg gtaggcaact
    10681 acatcgggtt tttcactccc gattaaaccg ttcactacac cttttgtggt tttcgagttg
    10741 aagataaacc catccacgct ttgcagatat cgcttttcga tgggacgata taaatcgttc
    10801 tgccattttg ggcgcaattc agaacagcgc aggtgatgga cgagcgaaat aatggggtag
    10861 ggatgccgcc cgtggtttgc gccgatgagc gatggatgat tgagctcatc ttgaatcaaa
    10921 atatcgagat ttggcggcag tttgaaagtg aggttatctg tcaggtgcgc ggcgtaattc
    10981 cgccacggca acgagatgat ctccactgaa tcaccttgtg cgcgcagata ttccaccaac
    11041 atgcggtcgt acatatatcc gccgctgaga gtgtcaatgg agccgtagat gacgagtccg
    11101 attttcattt ataagagttg aaaggttgta aagtttgaaa gttggaaggt tgaaactttc
    11161 taccctgcca acctttcaac ggaaaaagcg gcccatgcat tttcatgttc ccacagcact
    11221 accttgagct tggaaatgtt cctggccttg atgcgctcgt tcaacgtctc tgccagaatg
    11281 cgtgaaaaat gttcaatgga tggattcaag cctgcaaact caggtttctc atttaacatc
    11341 tgctctttgt aatagcccac cacatcatca aggtgttttt ccacatccac aatgtcgacg
    11401 agatagccgt gttgatcgag ttcactgccc ttgagttgca attcaaggat gtaatggtgc
    11461 gaatttggaa aattctctgg cccccaatct ccgccgatca aaaagtgacg tgcaatgaat
    11521 tctcttctta ctccaagggt gtacataaaa tgctcctgtg ttggtgtcag gtgtcaattt
    11581 tcgggtttta ggttgagcgc ctgcatgatc aaaaatccat tttttcgttc aatgggcatg
    11641 gcaaagttaa aggcgttgat gcgtgtgcaa aggtcaagat cagatcttgg gaattgagga
    11701 tgatcattca tgtgctggaa tgcatctcgc gattgcacca cgcggcggat cagttcttcc
    11761 gaatccactg cctgacctgt gaactgtttt tcaaaaaatt cagcacaggc caaatcctct
    11821 gcattgtccg ctccgcctgt gacaacgaaa gtgacctctt ctggccgcaa tcgcaagaca
    11881 tgctccatcg ttgcctgtgc cacaacaaaa ctggcggcaa acattacttc cgcatggacg
    11941 ctgtgaaccg cgccttgtgt acctgcacct gtgcgttgaa tcagggtaat tccagtcaga
    12001 tcatgttcga ggatgcgagt gggggagttc ccaaaatcga agcctgcagg cggcaacccg
    12061 cctacttcgc ccatcacttt tgaatttggg agttgggctt tcaactccaa tgcttcttcc
    12121 actgcgctga ccagtcgaat ctccctggct ccgcgtgaaa aggcgtatgc cgctgtggag
    12181 aacgcccgca atacatcaat gacaaggacg atgcccgtgg cggtatggca ggtttcgaga
    12241 gtggtgtaat tgaatttcat tttaaataat caaacacaac ctgaatggtt tcgtgcggtt
    12301 tttcatccag tagttgatag gcttttgcgg catctgcaat tgagaagcgt tgagtaatcc
    12361 atttttcggg gcgaatttgt tcgagagcct gccatgccac atcaaagcgg cgggatttat
    12421 cccagcgccc cgatagttct ggtgaaatcg tgctgacttg tgacgagatc aatctaatgc
    12481 gcgaacggtg aaatgacccg ccaaagtcga tctctgtccg cttttgtcca taccatgacc
    12541 caatgacaat ccgcccgctg aagatggtca actcaatggc atcgttcaat gcagaagggg
    12601 agccgctcag ttccagggtc aaatcgtact tgttttgcgt ataggcaact gaatcggtgt
    12661 ggaaggtttt gaggctggaa tccagagaat gaacaccttt aatctgcatt gccgccttgc
    12721 ggcgcagttc ataaaagtct gcggtgagca ggctttcgag tggaaattca cttaataaag
    12781 aagaggtcaa taaaccaacg acaccctgtc ccaacaccag gacgcgctca cccaaaatag
    12841 gggcagcgtc ctgcaccagg tttactgcgg tttccatgtt agggaggaag caggcatttt
    12901 cagaggttag ggaagcgggg atgggaatca aagatgaagg attaataatg aaggatgagg
    12961 catgcggatg aaacgcaaag acatgcttgt ctttccactc ttggcttaca tccgccccaa
    13021 tttcctttac tttccccaca catgcatacc cataggccag cggataattc aagtcgctgc
    13081 tgaaagtgtc gtgagaatcg gccaaatgag ggaattgccc acgatagacc agcatctcgg
    13141 tccctgcact gatggcggag cagatcgttt ccacgaggac ttcatctttt ttcagtgggg
    13201 gaagggtgat ctctttaatt tcaacttgtt tcggggctgt gaaaaaaatt gtctgtgctt
    13261 ttggcatggg cagagtttaa cataaaaatg acagccgctt tatgaggcgg ctgtcattgt
    13321 gtgctcggtt tatttgttgg gcacgggttt ggtcaggtaa ctgggcaggt cacgacgaaa
    13381 ctcgtatttt tccatgatct cttcaatgac gggcttttgt ttgggtgtaa cttccttgaa
    13441 ttcgaaccct acattgaaat attcggggct gatatcttgc tgacaccagg ccgcccgtgc
    13501 agggatggta atacggggga tcttcacatt ttccaattca ggcagttcga tggcgagggt
    13561 caattcggtg ttttctgcaa tgggcttttc gccgatcacc atggcaccat gaaggtttag
    13621 gtcacccaga tagccaagta aatgcccgcc attcaaatcg aagacctggg tgtaagccat
    13681 caaattcttg cgctgttgtt tgcgtctttc ctgcattgga cctctcacat gggcgtgggt
    13741 atctttttta ttctacaata catcatcacg ggattcattg cgattttgtg gtaaaactgg
    13801 cggatgcgcc tcatttatat ctccccccat ctcgacgatg ccattctttc tgctggcggc
    13861 ctgatccacg atcaggtgca agctggcagg catgtggaaa tctggacggt cttttgcggt
    13921 tttccgcctg ccaatcaact ttcctccttc gcacagatta ttcaccaaaa atggggcatc
    13981 atggatacga atgaactggt cgctgcacgc cgtgccgaag acaaggccgc ggctgatatt
    14041 gtcggtgcac aggtatttca tttcgatttt ctcgattgca tctatcggcg cggcagggat
    14101 gggaactggc tgtattcgga tgtattcatt cctccacata ccgatgatga cggcctgccg
    14161 gcgcagattg cagaagccat ttctgcccgc ctcaaccctg acgatgaact ggtctgccaa
    14221 cttgcgctgg gtgcccacct tgaccatacc ctcgtccgcc gcgcggctga gttgcttggc
    14281 cgcccactga cctatgacgt ggatattcca taccttttca acttccccga tgattttgcg
    14341 ttgaaaacgg ccaaaatgaa ggaaatcact cacaggataa ccgaagcagg cctacggtca
    14401 tggggagatg cgatccttgc gtatacctct caattcggca ccctgttttc cagtcccgaa
    14461 gagatgcgag aaaagcttca gcagtattgg gctgaaaagc aaggtatttc cttctggaaa
    14521 tttgagtaga caaatttact ggtgcttttg gggcttgtaa ccccttttaa tctatgatat
    14581 agtattgacg aatttccgga gatgaaaccg gaaattttta tccattgcat cccttttggg
    14641 gtgcaaaatt ctatatagca ggacaaacca tgccaacaac caatttcctt accaaagagg
    14701 gctttcagaa gcttcaagag gaactggatc atttgcgcac agtgaaacga caggaagtag
    14761 ccgatcgctt gcacgaggct atggaaggcg gggaactgat cgaaaatgca gaatacgagg
    14821 cggccaagaa cgaacaggct tttgtagaag gccgtattca ggaacttgaa attttgcttg
    14881 ccaccgccaa cattatcgaa gaaaacaacg gcaaagccaa gaaaaatgat ttgattcacg
    14941 tcggttcaaa ggtgaccatt aaagagggga actttgaagc cgagaccttt accattgtgg
    15001 gggcggctga agccaaccca cgcgagggaa agatttcgaa tgagtcgccg attggcaagg
    15061 ccattctcaa tcacaagctg ggtgatatcg tgaaagtgga aacccctggc ggtacttaca
    15121 acgtgaagat catcaaggtc ggctaaacag gacgcgcaac gcttaacttg cccgcgtcca
    15181 atttgcgttg gatgcgggct ttttatttga aaaatacttt cttgaaaaca ggaaggaaga
    15241 agaaaatcat ggctgaatac aactcactgg aaaaaatccg tttgcagaaa attgaagaac
    15301 tccgcgccga gggcatcgag tcctacccta cacgcgccga gcggactcat accaatgcga
    15361 aggtcattgc cgagttcgag gccgctgaaa agaatgccgc tcccgatacg acccccgaag
    15421 tgaaggccgt gttggtgggg cgtattcgtg ccaatcgcgc aatggggaag gtttcttttg
    15481 cgcacattga agatggcagc ggcaagatcc agcttttctt tcgcagcaat gaattgggca
    15541 aggaacgcct tgacctgttc aacaaaatgt ttgacctggg agattttata caggcatcag
    15601 gagtcatgtt ccgcaccaaa acaggtgagg tctcgctgca tgtccatgac ttcaaattgc
    15661 ttgccaaatc tgtaacgccc ctgcccgctg acaaggatgt cacccaggag gatggtacgg
    15721 taacccgcta cgccgctttg gaatcacctg agctgcgtgc ccgccagcgt tatgctgacc
    15781 tggctgttaa ccctgatgtg cgcgagacct tccgcaaacg ggccacgttg atcaaggctt
    15841 tgaggaattt cctcgacggt catgattttc ttgaggtgga aacgcccatc ttgcagcctt
    15901 tgtatggcgg ggcggctgca cgtccatttg tgacgcatca taatgaactc gaccaggata
    15961 tgtacctgag gatttcgttc gagttgtacc tcaaacgcct gctggtgggg aatttggagc
    16021 gtgtgtacga gatcggacgc gatttccgta atgaaggcgt gtctttcaaa cataaccctg
    16081 agttcaccca gcttgagttc tactgggctt atgccgatta tttgcaggtc atggagttga
    16141 ctgagcagat ggttgcttat gtggccgagc aggtgacagg ctccaccaag gtgaccttcg
    16201 gcgagtatga gttggaattc aaaccgcctt ggaatcgtgt ggaaatgcga cagggtattc
    16261 ttgaaaccag cggcattgac attgccgaac acaaaacggc tgaggagctt ttcaaagcca
    16321 ttcgagccaa acatccgaac aaaacgcccg atccaaaatc ggcacgcggc aagatgatcg
    16381 attttctgct gggtgagttc cttgagccga ccttgatcca gccaacattc ctctacaact
    16441 atccacgcga tatttcgccg ctggcaaaat ccattcctgg tgacccgctg accgtggagc
    16501 gttttgaagg atatgcggct gggtttgagt tgtgcaatgc ctttactgaa ttgaacgatc
    16561 cgcttgatca agagcagcgg tttattgaaa tgggccgtga ttacacggat gatgaagaag
    16621 agaaacaccc gcttgatgaa gattatctgc gcgccatgcg gtatggcatg cctcccaatg
    16681 gtggtttcgg catgggtgtg gaccgtcttg ccatgctgtt gacaaataaa cactccatcc
    16741 gtgaggtgct gctgttccct gctttgcgca aggaagagtg aggtctttgt gcattacatt
    16801 tctcccgata aggaagaaca gatcaggcag atcgcccgaa gcggcaatct gatcgaggcg
    16861 atcaaagaat atcgcctgtt gaccaacgca agcttgaaag atgccaagag tgccgtggag
    16921 gcgatggtgc gtggaaatta tgtgaatggc cccgcacctc aagctgttgc caacggcgcc
    16981 gattcatctc aaaaaagcca gatcttggat ttaatgtcca agggtcggga gattgaggcg
    17041 atcaaactct atcgcggttg gaccaaggct ggcctgaaag aagccaagga tgcagttgag
    17101 gccatggcac ggggagagga tgtccagatc cctgagccag tgaccagcgt ggagagtggt
    17161 tcgatggatg gtcagatccg cgctttgttg gcgaagaatc aaaaaattga ggcgatcaag
    17221 gtacatcgtg ctgccaccaa ttccagtttg aaagaagcca aagactacgt ggaagccatc
    17281 gaaaggggag tgcggccaaa cccatcatct cccctgaata ttccgctcag caatgacccg
    17341 tttgaagagg agaatgttcg tacgcgccgc atgatggtct ttgtattcat catattggca
    17401 attctggccg ttgttttcgc attcgccttt ttgaggggtg gtttatagga gcatccatgc
    17461 aaaagtcaaa actcgttctt atatttggcg atgtgatcgc cattgccctg cttaccttca
    17521 tcggttttgc cacacatggc gaagccgatg tttccttttt gccgcgcatg gcggcggcat
    17581 tctttccagt attaatcggt tggtttgtgc tggcgccgtg gtttggtttg tttgatgagc
    17641 agattatttc caaccccagg tcgttgtggc ggatcctgcc tgccatgctg gctgtggcac
    17701 ctttggcggt gatgttgcgc gccgccattt tgaatgccgc cgctttgccg cttttcaccc
    17761 tagtgcttgg gagtaccaac gcatttggca tgttggtgtg gcgtgcattg catctctttg
    17821 ttgtaaaacg aaatgcacgc tgattcaata aatcggcaag tcgacgattc gcgggtaaaa
    17881 taaactcatg gatgaaccag cccttattca gaccgcacaa aatggggatc ttgattcttt
    17941 caataccctg atcctgcatt accaagaccg tgtttacaac accgccctgc gcatccttgg
    18001 cgatgaagac caggctgccg atgcggccca ggaagctttt atttccgcgt ttcgcagcat
    18061 caattcgttt cgcggcggat cgttcaaggc atggctcatg cgcactgtca ccaacgcctg
    18121 ctacgacgag ttgcgccgcc agaaacgacg ccccacaacc ccacttgaac ctgataccgc
    18181 cgacggcgag gagatggatt ctccacgctg gctggctgac cccaacatga cccctgctga
    18241 gaaatcggag gcggacgaac tcgagcacgc cattcagcat tgccttgatg cgcttccaac
    18301 agactttcgt acggtggttg tcatggcaga cttgcaaggc cttgattaca ccgaggtggc
    18361 agccgctgta cgtgtgccat tgggcaccat caagagtcgt ctggcacgtg cgcgtttgcg
    18421 attgcgtgag tgtctgcgtg gcttcgagga acttttgcct tcttcatttc gtctggaaga
    18481 ggaaagtaga ttatgaaaaa cttccgcgat attgaacaac tctcttccta tctggatgga
    18541 caactcagcc catctgattc tgcacgcgtt gagtcgcgca tcgcatcgga ccctgagctt
    18601 gcctctgcct tcgaagactt gcgcgccacc cgcggcattc tgcgtaaact gcctgcacgc
    18661 aaggctcccc gcaactttac cttgacccgt cagatggttg ggttgaaacc acccgtctcg
    18721 agatcgtatt cctttttccg cttttcaagt gccttcgcca ctgttttatt gatgctgacc
    18781 tttgccgcga attacgttgc tccgcgcatg tccgttgtgc ctgttggtgg ttcaggcggt
    18841 ggttgtgacc cctgcggcct tggcggcggt ggcggccccg aatcctttaa tatggatgcc
    18901 ctgcctgccg caacagaagc tccagcggtt gcggcggagt catttctcgc tacagaagcc
    18961 cccgctgcaa ctgaagcgcc agccatggtg gaggaaccag ctgctaccga ggcgcccgtg
    19021 gaggtccttc caaccatgga tatggctctg gccccgacag aattaccgct ctccacgcaa
    19081 gacacacagc ggatcgttga aacgccttcc gcttctccca aagaaccggt gatcgagtca
    19141 gcaccgcaac agcagccacc agcagaggat gaatcgttta tttctgccac atggcaggtg
    19201 gttttgctgg tcatcggtct ggtcagtgga ttggcggcct tcttcatgaa tcgttccgca
    19261 aagaaaaagt ggagttaaac attggctcga gatagccata ttaaattctg cccgcgttgc
    19321 ggggctgagg ttgcacacga agaaaagttt ggaatggtgc gcccgatctg ccctcaatgc
    19381 gggtggattt attttattga cccgaaggtg gcggcggcga ttcttgtaga gcaggaaggc
    19441 cgtgtgctgc ttgtgcggcg tgttaacgag cctttccgcg ggttgtggac tttgcccgca
    19501 gggtttgtga acggtggtga agacccagcc aaggctgctg cacgcgagtg cctggaagag
    19561 acaggtttac acgtgcatgt gaccggtgtt ttggatgtga ttgctggccg cgagcacgaa
    19621 cgtggcgcag atttcatcat cgtgtatcat gcggaagtga cgggtggtga gttggtgcct
    19681 gctgatgatg ctgatgcggc ggagtggttt ttgcggagcg aattgccgcc gctggctttc
    19741 aatgccacca aaaagatttt ggaatgatca tgaatgccca agactacggt tgggcgaaaa
    19801 acttgataac aaaaaagact tcggagtttt atctccgaag tcttttttgt tgaggatgat
    19861 tctgctgctg atcaagccgc tgttctcggc ggcggggacg ccgccgagcg cattgtttat
    19921 ttcgaccgac tacttagctc agaagtcttt ttatggtgcg ttttggtagg tgatgtattc
    19981 gtagcttgag gtgatgttgt tgtcaaaatc cacggcgtag aagccgatgg aatattgtcc
    20041 atctacaggg atgtaggatt cgaaccagaa gccttgttcg ccgtaggtga agacgtcatc
    20101 acttagttca ttggtgtaag tggccacgcc attcgcatcg aaggtgtagt attgaacgta
    20161 atcggtgaag cggtcgccga tctgcgggga gatggctacg gcggtgtcaa tgccgttgct
    20221 gtctgagtcg ggcagggcgt aaatgttgag caggttgcca tcgaggtcga aggtgagttt
    20281 ggcatcggtg ggtgtggtgg cattgaccgg ggtgtattgg ccgtataccg agtaggtggt
    20341 gacgccatcg gcgctttcgt attcatcggg ttcgaacagg gcataggcct gattcgttcc
    20401 gtctttgagg acgaagagat agggttccca ttcgtaactg atctcgatgg ggctttcgcc
    20461 gtaatcgggg gcattcactc cgtccacgct gatggtgttg ggcgcactga caaaggacat
    20521 atcggccagc caggtgacgt tggcatcctg attgtaaaag aagagtacga agtagatgta
    20581 ggaaacatca ccgctgactg tggtggagag ggtgagggtt tcgtcggcgg agatcacagt
    20641 gtcagaaagc tgaatcgggg caatggtcag gggagatgcg cccggggcat tgatctcatt
    20701 ggtgcgcggg ggagtgtagg cttgtccttc ctggggagta aattcctgcc cggtgtagtg
    20761 gtaagcgagg aattcatccc atgtggagag ttgcaggaag cgggccgcgg attcggcgta
    20821 gtagggtggg aaatcgctgt tgtattcggt gtaggcgtag atatcagaga tggggaagtg
    20881 gaaggagatg ccgttcgagc cagacattct ttgcccgtgt ttttcagcaa cgaccgccga
    20941 tgaaatggcg gctttcagct gctcggaggc ttggaatatg gctgggtcgc cggtggcatt
    21001 ggcgaggatt tcggagaaat tgccgaggtc aataaatggg gagggcgtat cttcgccgaa
    21061 gatgctgtaa tagctgcggg tatattcgcg agcctgcgct acgtaccgtt ggtcaatggt
    21121 ggccatgacc gagatgaatt ggttcattgc gccgatgaca tcagggatgc gtgcgctttc
    21181 cactgcgctc agggtggtgg cggcttccac ttcttcgatc tcagaactgc tggttgacct
    21241 gagggtgagc agggtatcct gtgtgatgta ggtggagatg atcgcctggc tgaccccgcg
    21301 tccatccatg ctggggtttt gtgccagttg tcccatccat gcggcgtatg accagccggt
    21361 ggagggaatt acttcttcgg aggcgatcat gtaattggag tagggataga gcgacccata
    21421 tacttccacc atgcccatca aacaggcatc gaaaccgatg atctcgaatt tttgtccgcc
    21481 catttgctgt tgaaccactt cgatggcctg aacaatttgc ggcatggaaa gactcgaacc
    21541 cgattgcagg tctgagaagc cgccggtcca gcctccaccg tggtcagaca tgatgagggc
    21601 gtatttcttg gctgggtagt tttgaatggt ccaggtcatg aaatcgacaa gggtttgcgg
    21661 gttgcccatg tccacttcgc cgaggttttg caccaccggt gagttgatct ggttgaggtc
    21721 ggagtcctgg gtgatcaaga ggcggcgggc atctgtccag tcaccatcgc catcgaagga
    21781 gccttcggcg cggtcgatct gcacgacgat gttcatttgc gtatttgagc cgacgagctc
    21841 catttcgttg atgtcgaacc acaggtcgcc ttcaaggatg tcatcatcgg catcggaata
    21901 gtagatgatc gtccattcgg ggaggttggt ggtatccgca gggatgatgg ggtcggtgga
    21961 gacgacaggc ggtgtggtga cacttgtgcc gccaaagatg gaatctccgt aaatgtataa
    22021 caggacgcct gcacaacata cgcaggcaat gcagatgaca accgctgcaa tgatcacggg
    22081 gatgccaatt tttgaagttg tgtttcccat atctcctccg ataactgttt tatttttagt
    22141 ttctgttcca gcataatagc acgataagat aagccaatgc aagaggtttt tgatgttata
    22201 tgaccaaagt acctttcgat ttgaacattt tcggggtatc cttgcggtca actttcaaga
    22261 ggagataagg agaatgaaca tgaaaaagca ataccgtatg ttgtttgcct ttgccgccct
    22321 gttgctgttg gtcagcattg cgtgcaatgg cggtgcggct cccacggctg caccggttcc
    22381 cacacttcca cccgctccca ccagtcagcc ccaatccaat aatccgcctt ccaataacaa
    22441 tcccatcgtg ccgcagcccc cggcgttgaa tgaacccccg ccttcgaatg gcaatagctc
    22501 ggacctggtt gtgttcaccg atgaaaacaa cctgctgtca tttgaagtgc ccggtgactg
    22561 gtactatgaa cagcagactt atgaacatgc ctacattgac cgtatcactt ccacagatgg
    22621 ttccgccatt attgacagcc ttgtctataa cgatggtcag gctttcacag gaaatgacaa
    22681 tggccgcttt gccttgcagt tgctgcacca atattacagc aacacgggca aggaaggtga
    22741 catcagcatt tccggcgatg agatgcagcc agatggctct gaaaaattga cctggacttc
    22801 gaagagcgga ggctattccg gcgtatcctt ctttgaactg cgcggtggtg atcgcatgac
    22861 cttcctgatg ttcacccttt attatgccaa tgatgcagat caagcagtga tcgacatcct
    22921 ggatcatgcc atcacgagct atacgatccc atagttgaac gtattgatta atggaaacaa
    22981 aaagaggctg gagaaatcca gcctcttttt cattttttct ttttattcgg ggatgaacag
    23041 gaacatatct gtcatttcgc agcgtgtgcc gtagccgctg ttggtgccag agagcagggt
    23101 caacgcaaat tgaccgttgg tgccctgatc tgcagacagg gtgtattctg tcacttcacc
    23161 atccacagag acataggcgc tcttgcctct caccgccagc gagaaatctg cttcggcggg
    23221 gtttccaaag ttcacacgcc cggggccgcg ggttttgcca acttcataaa cgcttgagcc
    23281 acggctcatg aagaatgcga tgcggccttt atcgaggaag accacatagt gatcgtcact
    23341 gccttcttga aggccaaaga caacgccgca gcctgaaagg tctggtgtgg aattcgagga
    23401 agaccacttg aagtgtccgg agaacacaag gtcagacgct tctttatcga tggtccacca
    23461 ttgatagtag ttgagttgtg cccattcttc ttcaaacgga tcgatcggag tgatttcacc
    23521 ctcggtggtg ctgacatagc cctcatcggc aaattcctgc aattgagttt caaatgcgct
    23581 ggtgtccacc gatggcacaa cctctaccgg agtgggggat gcttccacca cgggcggcgc
    23641 ttcagtggga gggattgcgg ttggcggtgg gttggtcggc ggaagcgggg ttggagttgc
    23701 atccgccttg ccaaaattac aggcaagcat ggcgaggatc aatgacgaaa ttgcaagtac
    23761 gcttgaaaga tttttgcttg ttttcatggc tcactccttt ttttgatttt tttattatat
    23821 cagtgtcata cagggacaat ataatccccc aattgtacac aag
//
LOCUS       DAOQJC010000060        22523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1034_length_22523_cov_10.158403, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000060 DAOQJC010000000
VERSION     DAOQJC010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 22523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22523
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1034_length_22523_cov_10.158403"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3083)
                     /gene="dnaE"
                     /locus_tag="PLE39_10655"
     CDS             complement(<1..3083)
                     /gene="dnaE"
                     /locus_tag="PLE39_10655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNE68833.1"
                     /translation="MSFAHLHVHTEYSLLDGFSNIKKLVHRVKEMEMPAVAITDHGTM
                     FGVIDFYKAAKAEGIKPIIGLEAYMAARGMKDKDSKLDRSSYHLLLLAENETGYKNLL
                     KISSAAQLEGFYYYPRIDRDFLAAHSEGLICTSGCMSAEIPRALLQDNPEEAVRRMNW
                     YYDVFGKDNFFVELQQHNIKEITDLNKKLLEMGARYEANYIATNDVHYVNQEDARLQD
                     ILLAIQTNSTYNDPERMRMTDDSYFLRSPAEMNRLFAEIPSSLSNTLLIAERCNVDLG
                     FKGYHLPEFPVPDGYTAETYLRSLCEAGAENRYKEDATSSKVKERLDYELSVVHTMGF
                     DAYFLIVWDLCRFARDHGIWYNARGSAAGSIIAYTLGITMVDPLEHALIFERFLNPGR
                     ISMPDIDLDFRDDRRHEMLEYCARKYGDDKVAQIITFGTMGTKAAIRDVARVMEIPLP
                     EVDKVAKLVPFVSGRATTMKDAMEVAEFKDIYEKQKHLRDVIDIAAKMEGTVRNAGTH
                     AAGVVISDKPILEYLPLHRPTSGAEETPIKSVTQFEMGILDSLGMLKVDFLGLITLTV
                     MARACDHIFKRHGIKFDLSNIPIDDPKAFELMGVGQTAGVFQVEGCLSGDTYIGHRTI
                     KELYADFKEREQAGTLGGRELLRTNSCYVDDGHFSLNVINKVIYSGVKPVYRLFADNN
                     TWIKATADHHFFTQRGWVTLGELKPETDTLLFKSNTSRSGKLCVVCGKPLKTATGKSQ
                     HCKSCSAHISSNPSKPEVRERISQSRIGGPAWNKGLTAETAKDTLWIKNLSIYNAAQK
                     GITLEERHGAERAAEIRAKLSARFSGTNNPMYGRPPKETKTYTKSGFREDLGHYVRSS
                     WEADMARVFKYLGWEYQYEPLTFELVKNNGSTITYTPDFYVAEQGVFYEIKGWMDDVS
                     AEKIALFQEQYPEHNLIVVDKTLFAEFQVKYSDLVEWECPQFPTDSEWVNIKGIEYVG
                     EEDTYDLQMQAPGNNFVANGFVVHNSGMTRWLVQMKPKNLDNIIAMVALYRPGPMA"
     gene            complement(3188..4081)
                     /locus_tag="PLE39_10660"
     CDS             complement(3188..4081)
                     /locus_tag="PLE39_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNE68834.1"
                     /translation="MFSNYITGLKQRVVKVAQTVNERANGFPWVLYNTVNKTLMPDTA
                     IMASAIAYISLFSLFPLILLSISLASFSFGFAMMDQHETIKQLEFVAPALGQLLGQNI
                     DQVIRTRGPVTGFALLSLIWSASTIFYTLTHTLNGIWNVEQRHSSWRIRGVAILFVLV
                     FIGPTLFLISLAGSVIANIRFWLPDLILPFGYGISLLLTLLLDVLLFMILYMILPHGS
                     ASWREVLPGASGAGLLWEFAKRTFLSFVSTYISITNLVYGTVATIIAFLLWAYISSLI
                     FIFGAYLSVSYWQLKQNPKTG"
     gene            complement(4084..6246)
                     /gene="recQ"
                     /locus_tag="PLE39_10665"
     CDS             complement(4084..6246)
                     /gene="recQ"
                     /locus_tag="PLE39_10665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNE68835.1"
                     /translation="MPTPESILKSIFGYDTFKPLQREIIANVQARRDTLVVMPTGGGK
                     SLTYQVPALMFDGLTVVVSPLIALMKDQVEQLNALGVPAVFLNSSLSQEEYQENMDSV
                     KSGQSKLLYVAPETLLTPRIYSLLSSLKLDLLAIDEAHCISEWGHDFRPEYRQLVDVR
                     RKFPSAVCMALTATATPRVRADIAASLGFTQSNEFLASFNRENLFIEVSPKREPIAQT
                     LRFLENFKDQSGIIYCFSRKQVDDLTATLARHQYSVRSYHAGLEDDERRRNQEAFIRD
                     DVQIIVATIAFGMGINKPNVRFVIHFDLPKSIESYYQEIGRAGRDGLPAHCLLLYNYG
                     DASKIRYFIDQKEEPERSASYQHLEAMTRYAEGSICRRKPLLSYFGETFAQENCGTCD
                     NCNNEPVDQQDITVAVQKFLSCVKRSGERFGAAHVVDILLGSKNEKIERYKHHELSTY
                     GIGRELTKSQWMHLSRQLVERGYLYQEPAYRVLSVTDQGLEMLKSREQVFGQINEARK
                     SERASARFKPSPDSEYDRSLFAQLRNRRKELADEAGLPPYVVFSDKTLVEMAMYFPQS
                     RESLLNISGVGKVKFERYGIPFLEIIKEYCRANKIDEKFKAPAREKDDSGRRYMAVGD
                     AYNAGESVQSLMKQYNVSADTILNHLARFIMAGNPLRSAEDLIALSNLEPDQQMAVFK
                     AFDKHGSDMLKPVYDAMNGKVNYDELRILRLCYLCEKR"
     assembly_gap    6348..6447
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6515..9715
                     /locus_tag="PLE39_10670"
     CDS             6515..9715
                     /locus_tag="PLE39_10670"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE68836.1"
                     /translation="MASIPISKTKIIPPRRRDELLTRKRLVDFLFDSLDKKLILVSAP
                     AGYGKTSLLIDFANQSDIACCWLALDEMDRDPQRFLAYLIAAIAERFPDFGAASVSML
                     GGLTSFEQDMEKMLVMLVNEAYEQIHEHFILVLDDFHLIENIQPIQSFLNRFLQLVDE
                     NCHVVISSRVLNSLPDLPLMVARDQVSGLGFADLAFHPDEIQSLLAQNNNLHISDDEA
                     RKMIETTEGWIAGLQFSGMGFLPPSHTGVGLSDFLGQQVLDRQPEYLRDFILRTSMLG
                     EFDSSLCETVLAPLYAEPQNWHRLIASVVQNNLFVLPVGDDGRWLRYHHLFRDFLRKR
                     FEQTHPEEVRPILQRLGQAYENLAEWEKAYYTYKQLNDTGLQAALIEHAAATMLLNAR
                     VTLESWLNDLPPSMLRTRPGLLSIRGTILDTKGQAREGLDLLNQAEQAFRAEQNVPGL
                     ARALLRRGSVHRFLGDFDATLRDAEESMQLTEAVDDLQVMYAEGLRLKALALYRLGHA
                     RQSVSFFESSLNLYIRLKHTPTIPILFMETGMAYQTIGSYAEAGTAYDKALQIWQRDG
                     NLLWQASLLNNLAILHQSYGEYEKAALAFENGLLCARRSSYTRMEALISIGLGDMYSE
                     LEDFSIALTNYENAEEIARRINDRFLIFYTITARVRLALLQKDADGAHRIMEQDVELF
                     QSNASSRYERGLLALMRGHLALLTHENAEAVAHLEEALRCFSEDGRKLEGDTSRIWLA
                     AASHQNRNNELAAQVLKPLLGTQPAHPVLVAVHQAQMWLNGLQTHPEVGRMVGDLFIR
                     AARFSARIPADRRQLRRMAHVLQIPNPHMVIQAFGRAAVSVGGKTLSLSDWQTQSVRD
                     LFFYFLTASRAFTKEQIAETLWPEVDEPHKIRLRFKNEIYRLRRAVGQEIIAYEDIFY
                     KFNRSLDFEYDVEAFEAFLAHAKAAATPVEQIGYYKKAVDLVQGPFLEDVYADWATPE
                     RERLSQVYLSTLLTLGDLLLKQTQTEQALVICKRALDYDVTFEAAYALSMQVYHRMGD
                     RAAIVRTFQTCEEVMQRELGLPPSRETKELYKRLVM"
     assembly_gap    9758..9857
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10176..10319
                     /locus_tag="PLE39_10675"
     CDS             10176..10319
                     /locus_tag="PLE39_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68837.1"
                     /translation="MKQQAASIGIFLQRIDRQQIQLILAIASLVALALGSGAPLDGGS
                     GGG"
     gene            10320..10886
                     /locus_tag="PLE39_10680"
     CDS             10320..10886
                     /locus_tag="PLE39_10680"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HNE68838.1"
                     /translation="MILILFVAVGLLVGLLWAWFLKTPYHGPDLKAMWLALLGFLPQI
                     LVARLPNLLPNRMDAASLTISQVALLGFAWLNRHVAGMYLFICGAALNLLVMGANGGV
                     MPVSPQTADRLFSEEALLELSPGDRIGPKDILLLPQQTRLEWLADRFLPPAWFPFQAA
                     FSFGDVFIAVGVFWMLVRPNTLSGNRGT"
     gene            10888..11166
                     /locus_tag="PLE39_10685"
     CDS             10888..11166
                     /locus_tag="PLE39_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68839.1"
                     /translation="MLAPITIRRPASVNITAARPGVSRGLSRVFSAAVVNKQFCELLL
                     KDPQKALSRGYLGESFLLSKEEFDLILSIRASSLSDLARQVNRYQNGN"
     gene            11277..12761
                     /locus_tag="PLE39_10690"
     CDS             11277..12761
                     /locus_tag="PLE39_10690"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNE68840.1"
                     /translation="MITELSLQNLPDASRQLWAVFQAFDDQLFVLDADGVILDFKLGG
                     QVSHLPVSMDMLLMRKIQNIFPAEIGAKLADVLNKIRTGSRVSHLEYSLQTPSGRLWF
                     ESRLVALANGQIIMMVRDITRHKISEETIKRQFGQLAALRSIDMAITSDLGLKPVLSM
                     ILEQTRMQLGVDAASILLFDPQTNLLEFAADAGFFTKALQHTRLLVGEELAGRAIQDQ
                     SVVYVDNLLEQPTAFQRSPQFRLENFISYYAVPLISRGQPVGVLEIFHRARLETDQEW
                     LNYMETLAGQAAIAIDNGTLFKHLQQSNAELMLAYDKVIEGWSRALDLRNKETEEHSR
                     RVTELTVRLAERMGIAQGELTHIHRGAMLHDIGKVAIPDSILLKPGPLTDEEWTVMRS
                     HPLIAVELLAPIEHLVPALDIPRSHHEKWNGEGYPDQLSGWGIPLSARIFSVVDVFDA
                     LISDRPYRPGWPVDKALQYIKEQSEIHFDPHVVPIFIDMMESRV"
     gene            complement(12883..15132)
                     /locus_tag="PLE39_10695"
     CDS             complement(12883..15132)
                     /locus_tag="PLE39_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68841.1"
                     /translation="MNKNNLQIGEKIETSLSALILIFLIFFSYAFLFRMPYAGFYFNP
                     SEGRVDMVYVKTTPETSLQIGDILIKVGDITLDEFRKNRRLSFFENTRPEETVDILIR
                     RDGQEKTIHWVFPGFTSAEFLGRFFNIWWLAFIFWLFGFVGQILIRPKDTRRLLLIAA
                     NYITSIWLITGNLSSSHLMESSTLMHATSWLLLPVYTHLHWIFPRPLNGLSKNVLIPV
                     YGVSGALALGEILHILPRSFYVLGFLGALIGSLVLELLHFIRQKDQRQNVTMLAAGVF
                     LAFAPTILFGLLILFSGFPALGPITFLALPFMPLTYFIVISRNQLGGVEVRANRLISI
                     YAFLIILGSVLLFLIMPITLLPLFEGETAFVTLAISSLTSLISILVFPAFQAYVEQRF
                     LGIKLPYQNIQESFSNRIAASASINDLLLLLENDVFPSLFIRQFAFIQTSNGNLNVLL
                     AKNVSADEMPDEGRISQLVERSGKFIPTGGWIRLILPLKAGDSFIGFWLLGRRDPDDH
                     YPQAEIVILQSIANQTAIALSYVLQTEYLKSIYQDGINQYEDERMRLALLLHDSILNE
                     LAVLRMNLNEMEEPPAFQNAYQKLTSQIREIIFNLRPPMLEYGLKPAIEEMAETLMEH
                     SQRKIKVITTIETDGSRYQAGNMEEHIFRMVQEASENSARHAQGQTIQIKGVLMKGEI
                     SLSIEDDGMGFNTKDGLGIDTLVRKKHFGLAGILKRAQLIGAEVFINSIPEKGTHVQV
                     VWKPSPIQG"
     gene            complement(15311..15994)
                     /locus_tag="PLE39_10700"
     CDS             complement(15311..15994)
                     /locus_tag="PLE39_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE68842.1"
                     /translation="MTKKIRVAILDDHQSIVDGYIYRLEKDPQIEITATMAFGEELES
                     TLEKTPADVLLLDVNVPISKENQNPYPILHCIPKLLQTHPDLNILIISMHAERGLIRA
                     VMDAGASGYILKDDPAILKNLGNVVKIIAEGGVQYSQAAHDQYARYLSEEVLSPRQLE
                     TLSLSAAYPNSTTSDLANNLNVSHSTVRNLLSNAYFKLNVHNRAAAIAKARQMGLITP
                     NPPEATANQ"
     gene            16357..16899
                     /locus_tag="PLE39_10705"
     CDS             16357..16899
                     /locus_tag="PLE39_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68843.1"
                     /translation="MAGTQIREYLNPSNYDHFYELFHALSEVVLSKISVIRTACHILN
                     TLHAQTPQVFSNVDAATHLTAFNKFTVEFDADMRRFFWPQQGKDSTQVTSLSMARWRP
                     YHETAWDDMFTQMVAVLQPAVNSAFDHYKQTNSLSLGDSPYSENIKSLLVQMEAQIND
                     LSRLCYPQEVERFLFRPPRI"
     gene            16992..21020
                     /locus_tag="PLE39_10710"
     CDS             16992..21020
                     /locus_tag="PLE39_10710"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68844.1"
                     /translation="MLKNFVKLFSGDPTKKTADKFGDLVTKVNALEAQFEALSDEALR
                     AKTDEFKARLAQALQGLTPPPVGEKGSEEGREYRKFEQDALNELMPEAFAAVREASKR
                     TIGLRHYDVQVIGGGILHGGSIAEMKTGEGKTLVATLPVYLNAIAGRGVHIITVNDYL
                     ARRDARWMAPVYNMLGLSVGVLQMAAVTENGKKAFIVDFERESPHEDQEHLRMVDRVE
                     AYRADITFGTNSEFGFDYLRDNMAMRMESRVQRGHYFAIVDEVDNVLIDEARTPLIIS
                     GPASGDLEWYGRMAQVVKQLRPEDYEVDEKNRNVSLTEIGVSRVEAILGTQLLDPDRP
                     EDLTPEQARLTGYLQQALRAQYLFHRNKDYLVQGGKVVIVDEFTGRQMPGRRWSDGLH
                     QAVEAKEGVKVEPENVTYATITLQNYFRMYEKLAGMTGTALTEAEEFNKIYKIEVAPV
                     PTNLEYQSLRSDATLVEQKAKDEDGYQYSYYTARGGDADGKIFYRRKDYPDVIYRTVE
                     AKLRAIVTEIVRYHALGRPMLVGTTSVESSDRLSNRLKAEPVRRLLQVTLVREMYLRA
                     NNMEEDGRLIEALVPFNEPIDKITPDALRKFIAPFGSNNISLDDDNNLNTLLSVLRLP
                     ETAKSRLKTIIQGGVLHQVLNARKHTEESQIIAGAGAFGAVTIATNMAGRGVDIKLGG
                     ELAEEVISAVNRVLRKAGHNDPFDLTQEERRQALLKADTSHYGIYEAEVKLFLQYFED
                     MESVKKLGGLHVIGSERHEARRIDNQLRGRAARQGDPGSSRFYLSLQDDLMRLFGGDQ
                     VSNLMTRLKVDEALPLEVRLVSNIIEGSQTRVEGANFDVRKHLLEYDDVLNEQRTQIY
                     NQRDRIFVKEDLSEDVAGMLENEVTKRVDLGLEDEEGPWKLLAWLEQVQPPFESQNGL
                     FPSYGFKLILEQIKQDDVQQSMLGIISRAIQAESDHHLRAIEALVAKTRESFDTQTSE
                     RDDAIDAYFEGLRDREDAPRAQKIAEELSALVRLPLKLNNEMTRALGDDPDSVKEDIQ
                     EMVAGQLTALSAARLIGAIQNRVGEQLPWPNPLPSDWDELEDVIVQTAREGLSHKSER
                     LTAQIARDMETLFQRESADSDAGRLRMLLTLSQGTRATFDQKTHKQVRQLFLRFSYIF
                     LAAQLLEGREAPDIVEDIMGHLETAEETLRITWGQSEFTRLNQNATRLADFGPAARIA
                     FGESRLNDAVSGLSESDRESLIDAIGRYVLNEVHRQLLLSAFSELWVEYLTKVEALRI
                     SIGLEAYAQRDPLVQYKGRASEMFQQLLEDVRGLVIGRAFAARPRRVEITPIETAEGD
                     VVSAPAETQVQMAGNKKKRKRH"
     gene            21034..22074
                     /gene="add"
                     /locus_tag="PLE39_10715"
     CDS             21034..22074
                     /gene="add"
                     /locus_tag="PLE39_10715"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNE68845.1"
                     /translation="MLSHQTVPLNQYFSLPKVELHRHLEGSLRLTTMLDIARQHGVTV
                     PASMFNLSGLVQVQDQDPMTFTNFLEKFKTLRLFYKSPDVIHRVTREAVEDAAKDNIR
                     YLELRFTPVALSRAEGFPLHDVMDWVLTSAQEAAKKSNIKVGLIASVNRHESPELAEQ
                     VVWLAAEHMKDGMVGIDLAGNEAEFKSAPFRDIFKEAKQSGLHVTIHAGEWGPAENVR
                     DALVNFDAERIGHGVRVMEDENIVALAKERGVTFEVCVTSNYQSGVVKSLPQHPLPRM
                     MAAGLHVTVNTDDPSVSRITLSHEYQHVCEDMKVSFDALRQSVLHAVQASFTTQDEKT
                     KLVAALKKEMKM"
     gene            22289..>22523
                     /locus_tag="PLE39_10720"
     CDS             22289..>22523
                     /locus_tag="PLE39_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68846.1"
                     /translation="MQDLFKSRDVLKVGKKEYVYFRLDALEKAGLVKLSKLPYSIRIV
                     LEAALRQCNDKEITQEDVKNIAAWSPKGARPGIP"
BASE COUNT         5290 a         5531 c         5940 g         5562 t
ORIGIN      
        1 gccatcggtc cggggcggta caacgccacc atcgcgatga tgttatccag attcttcggt
       61 ttcatttgca cgagccagcg ggtcattccg ctgttatgta cgacaaatcc gttggcgaca
      121 aaattgttcc ctggcgcctg catttgaaga tcgtaggtat cttcttcacc cacatattca
      181 atacctttaa tgtttaccca ttcgctatca gtggggaatt gcgggcactc ccattcgacc
      241 aaatcagagt atttgacttg aaactctgca aacagggttt tgtcaaccac aatcaaatta
      301 tgttcagggt attgctcctg aaaaagagcg attttttccg cgctgacatc atccatccag
      361 cctttgattt cgtaaaagac tccctgttct gcgacgtaaa aatcgggggt gtaggtaatt
      421 gtgctcccgt tgtttttgac cagttcaaag gtcaatggct catattgata ttcccaaccc
      481 aaatacttga aaacacgcgc catatccgct tcccatgaag agcggacata atgtcccaag
      541 tcttcacgga aaccagattt ggtgtaggtt tttgtctctt ttggcggacg cccgtacatt
      601 gggttgtttg tgccggagaa acgggccgaa agtttggcgc ggatttcggc tgcccgttcc
      661 gcgccatgcc gctcttcaag cgtaattcct ttttgagcag cattgtaaat actcaggttt
      721 ttaatccaaa gcgtatcttt ggcagtttca gcggtaagac ctttgttcca tgcagggcca
      781 cctatgcggc tctgcgagat tctttcacgc acttccggtt tgcttgggtt cgaagaaata
      841 tgagccgagc aactcttgca gtgttgagat tttccagtag cagtttttag cggttttcca
      901 caaactacgc ataactttcc tgaacggctg gtattgcttt tgaaaagcag ggtgtcggtt
      961 tcgggtttaa gctcaccaag cgtaacccag ccacgctggg taaagaagtg atgatcggca
     1021 gttgctttaa tccaggtgtt gttatcagca aacagacgat acacaggttt gacaccgctg
     1081 taaataacct tgttaattac attgagactg aaatgtccat cgtccacgta acatgagttg
     1141 gtgcgcaaga gttcgcgtcc ccccagtgtc cctgcctgct cacgttcttt aaaatcggca
     1201 tacaattctt tgatggttcg atgtccaata taagtgtcac cgctcaaaca tccttccacc
     1261 tggaagacgc cagcagtttg ccccaccccc atcaattcga acgctttcgg gtcgtcgatc
     1321 gggatattgc tcaaatcaaa tttaatgcca tgtcgtttga agatgtgatc acaggcgcgt
     1381 gccatcacgg tcaatgtgat caggccgagg aagtccacct tcaacatgcc cagcgaatca
     1441 aggatgccca tttcaaattg agtcactgat ttgatcggcg tctcttccgc accggaagtg
     1501 gggcggtgca gcggcagata ttcaaggatg ggtttatcag agatgaccac acctgccgca
     1561 tgcgtacctg cgttgcggac ggtcccttcc atttttgcgg cgatgtcgat cacatcacgc
     1621 aggtgttttt gcttttcgta gatatccttg aactccgcca cttccattgc gtctttcatg
     1681 gtggtggcgc ggcccgaaac gaagggaacg agtttggcaa ccttatccac ttcgggcagc
     1741 ggaatttcca tcacacgcgc cacatcacgg atggcggctt ttgtccccat tgtgccgaag
     1801 gtgatgatct gtgccacttt gtcatcgccg tatttacggg cgcaatattc gagcatttcg
     1861 tggcggcggt cgtcgcggaa atcaaggtca atatcgggca tggagatacg cccagggttg
     1921 agaaagcgtt cgaagatgag cgcatgttcg agcggatcca ccatcgtgat gccgagggtg
     1981 taggcaatga tcgagccagc agccgagccg cgggcgttgt accagattcc atggtcacgg
     2041 gcgaagcggc atagatccca cacgatgagg aagtaggcat cgaatcccat ggtgtggacg
     2101 acgctcaatt catagtcgag tctttctttg acctttgaac tggttgcatc ttccttgtaa
     2161 cgattctccg ccccggcttc acacaacgaa cgcagatagg tttccgcagt gtatccatcg
     2221 ggcacgggga attcaggcag gtgatagcct ttgaaaccga ggtctacgtt gcagcgctcg
     2281 gcgatgagca gggtatttga aagcgaggag ggaatctctg cgaagagacg attcatctca
     2341 gcgggcgagc gcaggaagta cgagtcgtcg gtcatgcgca tgcgttcggg atcgttgtac
     2401 gtggagttgg tttgaatggc gagcagaata tcctgcaaac gcgcatcttc ctggttgacg
     2461 taatgcacgt cgttggtggc gatgtaattc gcctcgtacc gcgcgcccat ctccagcaat
     2521 ttcttgttca ggtcggtgat ctctttgatg ttgtgctgct gcaactcaac gaagaaattg
     2581 tctttgccaa agacatcgta ataccagttc atgcggcgca cggcttcttc gggattatcc
     2641 tgcagcagcg cgcgcggaat ttccgccgac atacaacccg aggtgcagat cagcccttcg
     2701 gaatgggcgg caaggaagtc acggtcaatg cgtggataat aatagaagcc ttccagttgc
     2761 gcagcagagg agatcttgag cagattcttg tagcctgttt cattttccgc aagcagcagc
     2821 aggtgatagg aagaacgatc gagttttgag tctttgtcct tcatgccgcg tgccgccatg
     2881 tacgcttcca acccgatgat gggtttgatt ccctctgctt tggcggcttt gtaaaaatcg
     2941 atcaccccaa acatggtgcc gtggtcggtg atcgccaccg caggcatttc catttccttg
     3001 acgcgatgaa ccagcttctt gatattcgag aagccatcca gcagggaata ttcagtatgg
     3061 acgtgtaagt gggcgaaaga catggaaggg tatcagtggt tctttaagta ttcagtagcc
     3121 agtgattcag cgggtggtca ttatagcacg catgttcaat catcgatctt gcatttggct
     3181 taaaaaatca gcctgttttt gggttttgtt tcaactgcca atatgaaacg ctcaaataag
     3241 ccccaaagat aaaaatgaga ctgctgatat aagcccacag caaaaaagcg atgatggttg
     3301 ccaccgtgcc atacaccagg tttgtaatgg agatgtaggt ggaaacaaaa gagagaaagg
     3361 tccgcttggc aaactcccat aacaatcccg ccccgcttgc accgggtaac acttcgcgcc
     3421 aggaagcgga gccatgcggc aggatcatgt acaggatcat gaacagcagc acatcgagca
     3481 gcaaagtgag gagcaggctg atgccatacc caaaaggcag aatcaggtct ggcagccaga
     3541 aacggatgtt ggcaatgaca ctgcctgcca gcgagatcag aaaaagggtt gggccaatga
     3601 acaccagaac gaacaggatc gccacgccgc gaatccgcca cgaagaatgc cgctgctcaa
     3661 cattccaaat gccgttcagg gtgtgtgtga gggtgtaaaa aatggtcgag gccgaccaaa
     3721 ttaaactgag cagcgcaaaa ccagtcacag ggccgcgggt gcgaatgacc tggtcaatgt
     3781 tttgcccaag caattgacca agtgcgggcg ctacaaattc cagttgtttg atggtttcat
     3841 gctggtccat catcgcaaaa ccaaaactga aacttgccag cgaaatgctc aacaggatta
     3901 aggggaaaag cgaaaaaagc gaaatgtagg caatggctga tgccatgatc gccgtgtcag
     3961 gcatcaacgt tttattgacc gtgttgtaca acacccacgg aaaaccgttc gcgcgttcgt
     4021 tcacggtctg cgcaactttg accacccttt gcttcaatcc agtgatgtag tttgagaaca
     4081 ttgttatctc ttttcacaca aatagcacaa ccgcaagatc cgcagttcat cgtaattcac
     4141 tttgccgttc atcgcatcgt aaacgggttt caacatgtct gaaccgtgtt tatcgaatgc
     4201 cttgaagact gccatttgct ggtcgggttc aaggttcgag agcgcgatca gatcctcggc
     4261 tgagcgcagc ggattgcccg ccatgatgaa ccgcgccaga tgattcagga tcgtatcggc
     4321 gctcacattg tattgcttca tcaaagactg cacactttca cccgcattat aggcatcacc
     4381 cactgccatg taccttcggc ctgagtcatc cttttcgcga gcgggcgctt tgaatttttc
     4441 atcgatcttg ttcgccctgc aatattcctt gatgatctca aggaacggga tcccataacg
     4501 ctcgaacttc accttgccca cgccgctgat gttcaacagg ctttcacggc tttgcgggaa
     4561 atacattgcc atttccacca gcgtcttatc cgagaaaacc acataaggcg gaagacccgc
     4621 ttcatcggcc agttcttttc ttctatttcg aagctgggcg aatagggacc tgtcatattc
     4681 agaatcgggc gacggtttga accgcgcgct cgccctttca ctcttccgcg cttcattgat
     4741 ctgaccgaaa acctgctccc gcgatttgag catttccagc ccctgatcgg tgaccgacaa
     4801 cacccgatag gcaggctcct gatacagata tccccgttcc accagttgac ggctgagatg
     4861 catccactga ctcttggtca attccctgcc aatgccgtat gtggaaagtt catgatgttt
     4921 gtatctttcg atcttttcgt tctttgaacc gagcaggata tccaccacat gtgccgcgcc
     4981 gaatcgttcc ccgctgcgct tcacgcagga caggaatttt tgcacagcca ccgtgatgtc
     5041 ctgctggtcg acgggctcgt tattgcaatt gtcacaggtg ccgcaatttt cctgcgcaaa
     5101 ggtctcgcca aagtaggaaa gcagcggctt gcgcctgcaa atgcttcctt cggcataacg
     5161 ggtcatcgcc tccaagtgtt gataggaagc gctgcgttca ggttcctctt tctggtcgat
     5221 gaagtaacgg atcttgctgg catctccata gttgtacagc agcagacaat gtgcgggcag
     5281 gccatcgcgc cctgcgcgcc cgatctcttg atagtagctt tcaatgctct tgggtagatc
     5341 aaagtggatg acaaagcgca cattgggttt gttgatgccc attccaaagg cgatggttgc
     5401 cacgatgatc tgcacatcat cacggatgaa tgcctcttga ttgcgcctgc gttcgtcatc
     5461 ttccaaccct gcatggtatg agcggacgga gtattggtgc cgcgccaggg tggcggtgag
     5521 atcatccacc tgcttgcgcg aaaaacagta gatgatgccc gactggtcct tgaagttttc
     5581 gagaaaacgc agggtctgtg caatgggttc gcgtttggga gatacttcga tgaacaggtt
     5641 ttccctatta aaagaagcga ggaattcatt ggattgtgtg aacccgaggg aggcggcaat
     5701 atcggcacgg actcgcggtg tagcggtggc cgtcagggcc atgcatacgg cagagggaaa
     5761 ttttcggcgc acgtccacca gttggcggta ttcggggcgg aagtcgtgtc cccactctga
     5821 gatgcagtga gcttcgtcga tcgcaagcag atcaagtttc agcgaggaaa gaagcgagta
     5881 gatcctggga gtcagcaatg tttcaggcgc cacatacagc agcttggatt gtccgctttt
     5941 cacagagtcc atgttttcct gatattcctc ttgtgagagc gaactgttga ggaaaacggc
     6001 aggcacaccc agggcattga gttgttccac ctggtctttc atcagcgcga tcaaagggga
     6061 gacaacaacg gtcaatccgt caaacatcaa cgcggggact tgatatgtga gcgattttcc
     6121 gccgcccgtg ggcatgacaa ccagtgtgtc gcgccgggcc tgcacattgg caatgatctc
     6181 acgttgcagc ggcttgaagg tgtcgtaacc aaatatggat tttagaatgg attcgggtgt
     6241 gggcatgtcg gcctgattat aatgttactg cattcgtttt caggctttgt tccatgtaaa
     6301 atgagggatg gaaagaacat ccaaatgtaa aacaaaagca ttcttcgnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnccg catggtttgg cggtgaatta agaaccatcc
     6481 ttttcgaacc tctcttttta tattgggtac actgatggca tccattccga tcagcaagac
     6541 aaagatcatc ccaccccggc gccgtgacga gttgctcacc cgcaaacggc tggtggattt
     6601 tctattcgat tcgttggata aaaaattgat ccttgtgtct gcgcctgctg gctatggaaa
     6661 gacatctctg ttgatcgatt ttgcaaacca gagtgacatt gcctgttgct ggcttgcgct
     6721 cgatgaaatg gaccgtgacc cacagcgctt ccttgcttat ttgatcgcag ccattgcaga
     6781 gcgttttcca gattttgggg cagcatcggt ttcaatgctt ggcggcctga cctcttttga
     6841 gcaggacatg gaaaaaatgc tggtgatgct ggtgaatgaa gcctatgagc agatccacga
     6901 acattttatt ctggtgttgg atgattttca cctgatcgaa aatattcagc cgatccagtc
     6961 ttttctcaat cgtttcctgc aattggtgga tgagaattgc catgtggtca tttcttcgcg
     7021 ggtcttgaac agcctgcccg acctgccctt gatggtggcg cgtgaccagg tgagcggttt
     7081 gggttttgcc gatctcgctt tccaccctga tgagatccaa tccttgctgg cgcagaacaa
     7141 caacctgcac atctcggatg acgaagcgcg taagatgatc gaaaccacgg aaggctggat
     7201 cgcaggcctg caattttctg gcatggggtt tctgccgcct tcgcacactg gtgtgggtct
     7261 ttcagatttc ctcggtcagc aagtgctgga ccgtcaacct gagtatctgc gtgatttcat
     7321 tttgcgcaca tccatgctgg gtgagttcga ttcatccctg tgtgagacgg tccttgctcc
     7381 gttatatgca gaacctcaaa attggcatcg cctgatcgcc agtgtggtgc aaaacaatct
     7441 ctttgtgctg cccgtgggtg acgatggacg ctggctgcga tatcaccacc tgttccgtga
     7501 ttttttgcgg aaacgcttcg agcagactca tccagaagag gtccgcccga tcttgcagcg
     7561 gcttggtcag gcctacgaaa acctggccga atgggaaaag gcctactata cctacaaaca
     7621 attaaatgat actggtttgc aggcggcatt gatcgaacac gccgctgcca ccatgctgtt
     7681 gaatgcccgc gtaactttgg agtcatggct gaacgacctg cccccatcca tgctgcggac
     7741 acgccccggc ctgctttcca tccgcggcac aattttggat acaaaaggac aggcacgcga
     7801 aggactcgac ctgttaaatc aagcggaaca ggcttttcgt gcagaacaaa acgtgccagg
     7861 gttggcgcgg gctttgttgc gacggggcag cgtacaccgc ttcctcggag atttcgatgc
     7921 caccctgcgc gatgccgagg aatccatgca gttgaccgaa gcggtggatg acctgcaggt
     7981 catgtatgca gaaggtctgc gcctcaaggc gttggctttg tatcgtttgg gtcatgcgcg
     8041 tcaatcggtc agtttctttg aaagttcact gaatctctac attcgtttaa agcatacccc
     8101 taccatcccg atccttttca tggagacggg catggcctat caaaccattg gaagctatgc
     8161 cgaggctggc actgcctacg ataaggcatt gcagatctgg cagcgtgatg ggaacctgct
     8221 ctggcaggcc agtttgttga ataaccttgc catcctgcac caatcctacg gggaatatga
     8281 gaaagccgcc ctcgcttttg aaaatggatt gctgtgtgcc cgtcgcagca gctatacccg
     8341 catggaagcc ctgatctcca tcggccttgg tgatatgtat tccgaactgg aagatttcag
     8401 catcgccctg accaattacg aaaatgcaga agagatcgcc cgcagaatta atgaccgctt
     8461 cctgattttt tacacgatta cggcacgggt caggctggcc ttgttgcaaa aagatgcgga
     8521 cggggcgcat cggatcatgg aacaggacgt ggaactgttt cagtcaaacg catcatcccg
     8581 ctacgaaaga gggctgctcg cgctcatgcg cggacatctc gcacttttga cccatgaaaa
     8641 tgcggaagct gtagctcatc tcgaagaggc tttgcgttgt ttctctgaag atggccgcaa
     8701 actggaaggg gataccagcc gtatctggct ggcggcggca tctcatcaaa accgtaataa
     8761 tgagctggct gcacaggtgc tcaaaccact tttgggaaca cagcccgctc accccgttct
     8821 tgtagcagtt catcaggcgc agatgtggct gaatggactg caaactcacc cggaagttgg
     8881 ccgtatggtg ggtgacctgt tcatccgtgc cgctcgattc tctgccagaa tccccgccga
     8941 caggcggcag cttcgccgca tggcacacgt gttgcagatt ccaaatccgc atatggtcat
     9001 tcaggcgttt ggccgggctg ctgtcagtgt ggggggcaag accctctctc tttccgattg
     9061 gcagacccag tcagtgcgcg atctcttttt ctatttcctc accgcctcgc gtgccttcac
     9121 aaaagaacag atcgccgaaa cgctctggcc cgaagtggat gaacctcaca agatcaggct
     9181 gcgtttcaaa aacgagatct accgcctgcg gcgcgcagtt gggcaggaga tcattgccta
     9241 cgaagatatc ttctataaat tcaaccgttc gcttgatttt gaatatgatg tggaagcttt
     9301 cgaagcattc cttgctcatg ccaaagccgc cgccaccccc gttgaacaga tcgggtatta
     9361 caaaaaagca gtggaccttg tacagggtcc gttccttgag gatgtgtatg ccgattgggc
     9421 caccccggaa cgtgagcggt taagtcaggt ttatctctca acccttctta ctctgggcga
     9481 tcttctttta aaacaaactc aaacagagca ggctttggta atatgcaaac gggcattgga
     9541 ttacgatgtc acctttgagg ccgcatacgc cctttccatg caggtgtatc accgcatggg
     9601 cgaccgtgcc gcgatcgtgc ggaccttcca aacctgcgag gaggtgatgc agcgtgaact
     9661 tggcctgccg ccttcaaggg aaacaaaaga actctacaaa cgcctggtca tgtaatctca
     9721 tttcaagata aaaattccgt acatttgcac cgcgaagnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnatt taatagaaga acagggcaca aagctcgtgg agtactgaaa
     9901 atccgcgagt tttgcgtctt gttttgtttt aacttaccca ctgcctttat ctaattgtta
     9961 cggcttttgg ctgctcaagc cgccgtcctc ggcagcgggg acggcggctt ggaaatgcaa
    10021 aaggaggttc attttcaggg gaagaacggc tcaaagtcgc agaatcagtt ttcggcgggg
    10081 atcgaatcgg aaattcctct taaaagcatg ggggtttgaa gcctgttttg aagcctggtg
    10141 gctcgtatcc tgttcgcaac gaaaggatgt gtaccatgaa acaacaggct gcctccatcg
    10201 gtatttttct tcagagaata gatcgccagc aaattcagtt aattcttgcc attgcttctc
    10261 ttgtggcgct ggcccttggg tctggtgctc ctcttgatgg cggcagcggc ggcggataag
    10321 tgatcttaat tttattcgtt gctgttgggc tcctggttgg ccttttatgg gcgtggttcc
    10381 tgaaaacgcc ctaccatggg cctgatctga aggccatgtg gctggctctt ttggggttcc
    10441 tgcctcaaat tttggttgcc cgcctgccca accttttgcc aaaccgcatg gatgcggcca
    10501 gcctcaccat ctcacaggtc gcgctgcttg ggtttgcatg gctaaatcgg catgttgctg
    10561 gaatgtatct tttcatctgt ggcgcagcgc taaatctgct cgtcatgggg gcgaacggcg
    10621 gggtcatgcc cgtcagcccg caaactgccg accgcctgtt ttcagaagaa gccctgttgg
    10681 aactttcacc cggggaccgc atcggcccga aggatattct cctgcttccg cagcaaaccc
    10741 gcctcgaatg gcttgcggat cgtttcctac ccccggcctg gttcccgttt caagcggcat
    10801 tcagcttcgg tgacgttttt atcgcggtcg gcgtcttttg gatgttggtc cgcccaaata
    10861 ctttatcagg aaatagaggt acatgacatg cttgccccca tcaccatccg aagaccagca
    10921 tctgtaaaca tcactgcggc gcgtccgggc gtttcgcggg gattaagccg cgttttttca
    10981 gcggcggttg ttaacaagca gttttgcgag ttgctgctca aagaccccca gaaggcttta
    11041 agtagagggt atctgggtga atcgtttttg ctcagcaagg aagagtttga cctgattctc
    11101 tccatccgcg ccagttcact ttctgatctg gccaggcagg ttaatcgtta tcaaaatggc
    11161 aattgacatg catgatcgat tccgtccccg cagacaatgt atctgatgct ctcttttccc
    11221 aattttaaat atagaggtct gcctggagtc tttctttaat ggcgctttta aaaaaaatga
    11281 ttaccgaact ctctttgcaa aacctgccag atgccagccg ccaactttgg gctgtctttc
    11341 aggcctttga cgaccagcta tttgtcctcg atgcagatgg cgtgattttg gatttcaagt
    11401 tgggtggtca ggtcagccac ctgccggtgt caatggatat gttgttgatg cggaaaattc
    11461 agaacatctt ccccgctgag ataggcgcga aacttgccga cgtgttgaac aaaatccgca
    11521 ctgggagcag ggtctctcac ctcgaatact ccctgcaaac gccttctggc aggctgtggt
    11581 tcgaatcacg actggtggct cttgccaatg gacagatcat catgatggtg cgcgatatca
    11641 cccgccacaa gatatctgaa gaaacgatca agcgccagtt tgggcagttg gccgccctgc
    11701 gctccattga tatggcgatc acctctgacc ttggcttgaa accagttctt tcgatgatcc
    11761 ttgaacaaac ccgtatgcaa ttgggcgtgg atgccgccag cattctgttg ttcgacccgc
    11821 agacaaatct gcttgaattt gccgcagatg cggggttctt cacaaaagct ttgcagcata
    11881 cccgtctgct ggttggggag gaacttgccg ggcgggccat acaggatcag tcggtggtct
    11941 atgttgataa cctgctcgaa cagcccacgg catttcaaag atcgccccag tttcggctgg
    12001 aaaatttcat ctcctattat gctgtgccgt tgatttccag agggcaaccc gttggcgtgt
    12061 tggagatttt ccatcgtgcc cgccttgaaa cagatcagga atggctgaac tatatggaga
    12121 ccctggcagg gcaggcagcc attgccattg ataatggcac gcttttcaag caccttcaac
    12181 aatccaatgc ggaattgatg ctggcctacg acaaggtcat cgaaggctgg tcgcgggcat
    12241 tggatctgcg caacaaggaa accgaagaac attcgcgccg tgtgactgaa ttgaccgtca
    12301 ggctggcaga acgcatggga attgcccagg gcgaacttac acacatccat cgcggcgcca
    12361 tgctgcatga tatcggcaag gtcgcaattc cagatagcat cctgcttaaa cctggtccct
    12421 tgaccgatga agaatggacg gtcatgcgga gccacccgct gatcgcggtg gaactgctcg
    12481 cgccgatcga gcatctcgtg cccgctttgg atattccccg ctcacaccat gaaaagtgga
    12541 atggtgaagg ctacccggac caactttcag gctggggaat tccactctca gcccgcattt
    12601 tttcagtggt ggatgtgttc gatgccctca tatcagatcg tccctaccgc ccgggttggc
    12661 cggtggataa ggctctgcaa tacatcaagg agcaatcaga aatacatttt gacccgcatg
    12721 ttgtgcccat tttcatcgat atgatggaaa gccgggttta atttcaacat accgatttga
    12781 acaaaggggg aaaggggata gggtgcgctt caacttcttg ggtggctggt catcattgga
    12841 gcaagagtgg tgaggttgaa gcgcgcctgg atatttacga gattaccctt gtatggggga
    12901 aggcttccaa acaacctgaa catgggtgcc tttttcgggg attgaattga tgaaaacctc
    12961 cgccccgatc aactgggcgc gtttcaaaat tcccgccaga ccgaaatgct ttttcctgac
    13021 aagggtatca attcccaacc cgtcttttgt attaaacccc atcccgtcgt cttcaataga
    13081 gagtgagatt tcacccttca tcaacacacc cttgatctgg atcgtctggc cctgtgcatg
    13141 gcgtgccgaa ttttcactgg cctcctgcac catgcggaag atatgttcct ccatgttccc
    13201 agcctggtat cttgagccgt cggtttcaat cgttgtgatg accttgatct tccgctggct
    13261 gtgttccatc aaggtttctg ccatttcttc gatggcgggc ttcaacccgt attccagcat
    13321 gggcgggcgc aggttgaaaa tgatctcacg gatctgactc gtcagctttt gataagcatt
    13381 ttggaaagca gggggttctt ccatttcatt caggttcatg cgcagtacag ccagctcatt
    13441 gaggatgctg tcatgaagca gcagcgccag tcgcatgcgc tcatcttcgt actgattgat
    13501 cccatcctga tagatggatt tgagatactc cgtttgtaac acgtagctta atgcaatggc
    13561 ggtttggttt gcgatggatt gcaggatgac gatctctgcc tgtggatagt gatcgtccgg
    13621 gtcgcggcgt ccgagcagcc aaaagccgat gaaagagtca ccagctttca gggggagaat
    13681 gaggcggatc catcctcccg tggggatgaa tttcccgctt cgttctacca gttggctgat
    13741 tctgccttcg tctggcattt catccgctga gacatttttt gccagcagca cattcaaatt
    13801 cccattggag gtttgaatga aggcgaattg acggatgaac aaacttggaa atacatcgtt
    13861 ctcaagcagc aatagaagat cgttgatgga tgcactggcc gcaattcgat ttgaaaaact
    13921 ttcctgtatg ttttggtagg gaagtttgat tccgagaaat cgttgttcca cataggcttg
    13981 aaatgctggg aaaaccagaa tcgagatcag gctggtgaga gaggaaatgg ccagggttac
    14041 aaatgccgtc tcgccttcaa acaatggtaa aagcgtgatg ggcataatga ggaacagtaa
    14101 aacggagccg agaatgataa gaaaggcata aattgaaatc aggcggttgg cgcgaacttc
    14161 caccccgccg agttggttgc gtgaaatgac gatgaaatag gtcaggggca tgaatggcag
    14221 ggccaggaat gtgatggggc ccagggctgg aaacccgctg aataaaatta acaacccaaa
    14281 caggatggtt ggtgcaaaag ccagaaagac accagccgca agcatggtca cattttggcg
    14341 ctgatctttt tgacggatga aatgaagcaa ttcaagcacg aggcttccga tcaatgcccc
    14401 aagaaaacca aggacataga aactgcgcgg caggatgtgc aatatctcgc ccagtgccaa
    14461 agcaccgctc acaccgtaaa cagggatcaa cacatttttt gataacccat tcaaaggacg
    14521 cgggaagatc caatgcaggt gggtgtagac aggcaggaga agccagcttg tggcgtgcat
    14581 cagggtggaa ctttccatga gatgcgagga ggataaatta ccagtgatca accagatgct
    14641 ggtgatgtag ttggccgcaa taagcaggag tctgcgtgtg tcttttggac ggatcaagat
    14701 ctgtccaacg aatccgaaca gccaaaaaat aaaagccagc caccaaatgt tgaaaaaccg
    14761 cccgaggaac tcggcgctgg taaagcccgg aaagacccaa tggattgttt tttcctgccc
    14821 gtctcgacga atcaaaatat caaccgtttc ttcggggcgt gtgttttcga agaatgacag
    14881 cctgcggttc tttcgaaatt catcgagtgt aatatccccg acctttatga ggatatcgcc
    14941 gatttgcagg gaggtttcag gcgtggtctt tacatagacc atgtccacgc gcccttctga
    15001 agggttgaaa taaaaccctg cataaggcat cctgaataaa aaggcatagg aaaaaaatat
    15061 caggaagatc aatatcagcg cggaaaggga tgtttcaatc ttttccccaa tctggagatt
    15121 gtttttgttc atgggtttcc ttgctggaaa tgggtgggtt gataagggat aggaggccgt
    15181 ttcgattata gcgtaggaac atgccatgag caccactttt gtgtatggaa aaccgacctt
    15241 gcaggccttg aaagaggcat gtcaggtttt cacttaccaa caagtgatgt atgagattgt
    15301 ggggatctgt ttattgattg gctgttgctt ccggcggatt tggtgtaatt agccccattt
    15361 gccgcgcttt tgcgatggcg gcggcgcggt tgtgtacatt caatttgaag taggcgttgg
    15421 agagcaggtt gcggaccgtg gaatggctca cgttcaagtt gttagcaagg tcagatgttg
    15481 tggaattggg gtatgccgcg ctgagagaga gtgtttccag ttgacgaggg ctgagaactt
    15541 cttccgataa ataacgggca tattgatcgt gtgccgcctg gctgtattga accccgcctt
    15601 ctgcaatgat tttcaccaca ttgcccagat tcttaaggat ggccgggtcg tctttcaaaa
    15661 tatacccgct ggccccggca tccatcactg cgcggatcaa tccgcgctcg gcgtgcatgc
    15721 tgatgatgag gatgttaaga tcaggatgcg tttgcagtaa tttgggaatg caatgcagga
    15781 tcgggtaggg gttttgattc tcttttgata tcgggacgtt gacatccagc agaagcacat
    15841 ctgcaggtgt tttttccagc gtagattcca gttcctcccc aaacgccatg gtcgctgtta
    15901 tttcgatctg cgggtctttt tccagccgat aaatgtaccc atccacgata ctttgatgat
    15961 cgtccaggat cgccacgcgt attttttttg tcatatacca cactcccgct tgcatttaat
    16021 tcagggtagc agaaagaatc ctcggtttca agccagtatg caggattcag tacaaatgtc
    16081 tatgcctggt tgcacctgtc tattgaatcg ccttttgggt atctgtacaa tttacttaag
    16141 aatatcaggc ttaatcatgg atgaaatgag ccattgctct cattccattc atcatgggga
    16201 aaaggggata ggatgcgctt caacttcccg ggtggcttga acgtcagaag actggcggag
    16261 gaggttgaag tgcgtctgga tatgttcatt taaaagaagg tgggatagtg cgtggattga
    16321 gaaaacaaaa cccgcagcgg gtgatacaat ccctgcatgg caggaacaca aataagggaa
    16381 tacctgaatc catcaaacta cgaccatttt tacgagcttt ttcatgcatt atctgaagtg
    16441 gtgttgagca agatcagtgt gattcgcaca gcctgtcata ttctgaacac ccttcacgct
    16501 caaaccccgc aggttttttc aaatgttgat gctgctactc atctgaccgc atttaacaag
    16561 ttcactgtgg aatttgatgc ggacatgcgc aggtttttct ggccgcaaca aggcaaagat
    16621 tcgacccagg tcacatcttt gagtatggcg cgttggaggc cctatcatga aactgcatgg
    16681 gatgatatgt tcacgcaaat ggttgcagta cttcagcccg ccgtgaattc ggcttttgac
    16741 cattacaagc agaccaactc gctttcactg ggcgactctc catattcgga aaatatcaaa
    16801 agtctgttgg tgcaaatgga ggcgcagatc aatgacttat cccgtctgtg ctacccgcag
    16861 gaagttgagc gtttcctgtt ccgcccgccg cgcatatgaa aatggaatag cactgccccc
    16921 caaacattaa taaaactcaa atcccccata ccctctccga atgctataat cccttccctg
    16981 aaagggatat tatgcttaag aacttcgtaa aactattcag tggcgacccc acaaagaaaa
    17041 cggctgataa atttggcgac cttgtgacaa aggtgaatgc gcttgaagca caatttgagg
    17101 ctttgagtga cgaggccttg cgtgcaaaga cggatgaatt caaggcgcgc ctggcgcagg
    17161 cgttgcaggg actgacccct ccacccgttg gcgagaaggg aagcgaagag ggcagggagt
    17221 atcgcaagtt tgagcaggat gcgttgaacg agctcatgcc tgaggcattt gcggcggtgc
    17281 gcgaagcatc caaacggacc attggcctgc gtcactacga tgtgcaggtc atcggcggcg
    17341 gaattttgca cggcggttcc attgctgaaa tgaagacggg tgaaggcaaa acccttgtgg
    17401 ccaccctgcc tgtttatctc aatgcgatcg cggggcgcgg ggtccatatc attacggtca
    17461 atgattatct ggcgcgtcgt gatgcccgct ggatggctcc ggtctacaac atgcttggct
    17521 tgagtgtggg cgttttacaa atggccgccg tcaccgagaa tggaaagaaa gccttcattg
    17581 tggatttcga acgtgagtcg ccgcatgaag accaggaaca tctccgcatg gtggatcggg
    17641 tcgaggcgta tcgcgccgat atcacctttg gtacgaactc tgaatttggt ttcgattatc
    17701 tgcgcgacaa catggcaatg cgcatggaat cgcgcgtaca gcgtggtcac tactttgcga
    17761 tcgtggatga agtggataac gtgctgattg atgaagcgcg tacaccgttg atcatctccg
    17821 ggcccgcttc aggcgatctt gaatggtacg gacgcatggc acaggtggtg aagcaattac
    17881 gtcccgaaga ttatgaagtg gatgaaaaga atcgtaatgt ctctctcacc gagatcggtg
    17941 tcagccgtgt agaggctatt ttgggcaccc agcttctcga tccagaccga cccgaagacc
    18001 tcaccccgga gcaggcgcgt ctgacagggt atctccagca ggcattgcgt gcccaatatc
    18061 ttttccatcg taacaaggat tatcttgtgc agggcggcaa ggtcgtcatt gtagatgaat
    18121 tcaccgggcg gcagatgccc ggacgccgtt ggagcgacgg cctgcatcaa gcagtggaag
    18181 ccaaggaagg tgtgaaggtt gagcctgaaa atgtaaccta cgccaccatc accctgcaaa
    18241 actacttccg catgtatgaa aagctggcag gcatgaccgg tactgcgctc accgaagcgg
    18301 aggaattcaa caagatctac aagatcgaag tggcccccgt gcccaccaat cttgaatatc
    18361 agtccctgcg ttctgatgcc acgcttgtgg agcaaaaggc caaggacgag gatggttatc
    18421 aatattccta ttacaccgcc aggggcggcg atgccgatgg gaagatcttc taccgcagaa
    18481 aagattatcc cgatgtcatt taccgcacgg tcgaggcaaa gctgcgtgcc attgtgacgg
    18541 agatcgtccg ctatcatgcg ctcggacgcc cgatgctggt gggtaccact tcggtggaat
    18601 cttcggaccg actctcgaat cgactcaagg ccgagcctgt tcgccgttta ctgcaggtga
    18661 ctcttgtccg tgaaatgtac ctgcgtgcca ataatatgga agaggatggc cgcctgatcg
    18721 aggcgctggt gccgttcaac gagccaattg acaagatcac tccagacgca ttgcgcaaat
    18781 ttattgcgcc attcggttcg aacaatatca gccttgatga tgataacaac ctgaacacct
    18841 tgttgtcagt tttgcgcctg cctgaaacag cgaagagccg actgaagacg atcattcagg
    18901 gcggtgtgct acatcaagta ttgaatgcgc gcaaacacac ggaagaatcg cagatcatcg
    18961 ctggtgcagg tgcttttggc gcagtgacca tcgccaccaa catggccggt cgcggtgttg
    19021 atattaaact cggcggcgaa ttggctgaag aggtcatttc ggctgtgaat cgggtgttgc
    19081 gcaaggcggg gcacaacgat cccttcgatc tcacacagga agaacgccgt caggctttgt
    19141 tgaaagcaga cacctcacat tacggaattt acgaggccga ggtcaaactt ttcctgcaat
    19201 attttgaaga tatggaaagt gtgaagaagc tgggcggttt gcatgtcatt ggctcagagc
    19261 gccatgaagc acgacgtatc gataatcagc tgcgcggtcg tgctgcccgt cagggtgacc
    19321 ctggctcttc gcgtttttac ctctcgcttc aagatgatct gatgcgcctg tttggcggcg
    19381 accaggtcag caacctgatg acccgtctta aagtggatga agccctcccg ctggaagtac
    19441 gccttgtaag caacatcatt gaaggttcgc agacccgtgt ggaaggcgcg aatttcgatg
    19501 tccgcaagca tttgctggaa tacgatgatg tgctcaacga acagcgcaca cagatctaca
    19561 atcagcgcga ccgcatcttt gtgaaggaag acctgagtga agatgttgca ggcatgcttg
    19621 aaaatgaagt gaccaaacgt gtggatctcg gccttgagga tgaagaaggt ccgtggaaac
    19681 tgctggcctg gcttgaacag gttcagccgc ctttcgaatc acaaaatggt ctgttccctt
    19741 cctatggttt caaattgatc cttgagcaga tcaaacagga tgatgttcag caatcaatgc
    19801 tggggatcat ctctcgtgcc attcaggcag agagtgatca tcatctgcgt gccattgagg
    19861 cgttggttgc gaaaacccgc gaatccttcg acacccaaac ctccgagcga gacgatgcaa
    19921 ttgatgccta cttcgaaggc ctgcgcgacc gtgaagatgc tccgcgtgcc cagaagatcg
    19981 cagaagagct cagcgctctg gtgcgcctgc cgttaaaact taataatgaa atgacccgcg
    20041 ctcttggcga tgaccctgat tctgtgaaag aggatattca ggagatggtg gctgggcagt
    20101 tgactgccct gagtgccgct cgcctcattg gcgcgattca aaaccgtgtg ggtgagcagt
    20161 tgccgtggcc gaatccgctg ccttcggatt gggacgagtt ggaagatgtg atcgtgcaaa
    20221 ctgcgcgcga agggctttca cataagagtg aacgccttac cgcccagatc gctcgcgata
    20281 tggaaactct gttccagcgc gaatcggctg attcggatgc gggcaggttg cgcatgttgc
    20341 tgaccctttc acagggtacg cgggccacgt tcgaccaaaa gacgcacaaa caggtccgac
    20401 agttgttcct gcggttctcg tatattttcc tcgccgcgca attgctggaa gggcgcgaag
    20461 ctcccgacat tgtggaagac atcatggggc atcttgaaac ggcggaggaa accctgcgca
    20521 ttacctgggg acagagcgaa tttacccgtt tgaatcagaa tgccacccgt ctggcagatt
    20581 ttggccccgc tgcacggatc gcattcgggg agagtcggct caatgacgcg gtatcaggcc
    20641 tcagtgagtc agaccgcgag tctctcatcg atgccattgg aagatatgtc cttaatgaag
    20701 tccatcgcca gttgttattg agtgcgttct ccgaattgtg ggtggaatat ctcacaaagg
    20761 tggaggcgtt gcgtatttcc atcggactcg aggcttacgc acagcgtgac ccgctggtac
    20821 aatataaagg ccgtgcatct gaaatgttcc agcaattgct tgaagatgtg cgtggattgg
    20881 tgatcggacg tgcatttgcg gccagaccgc gccgtgtgga gatcactccc attgaaactg
    20941 ctgaaggtga tgtggtttct gcgcctgctg aaactcaggt gcaaatggct ggcaataaga
    21001 agaaacggaa acgacattag tttttgaggc aagttgttat cccaccaaac tgttccgctg
    21061 aatcaatatt tttcccttcc aaaggtggag ttacaccgtc atcttgaagg gtctctgcgc
    21121 ttgacgacca tgctggatat cgcccgccag catggcgtga cagttcctgc cagcatgttt
    21181 aacttgagcg gcctggtgca ggtgcaggat caggacccaa tgacgtttac gaattttctc
    21241 gaaaaattca aaacgctgcg gcttttttat aaatctcccg atgtcattca tcgcgttacg
    21301 cgcgaggcgg tggaggatgc ggcgaaggat aatatccgct accttgagct tcgttttaca
    21361 cctgtggcgc ttagccgcgc cgaggggttt ccgctgcatg atgtaatgga ttgggtgttg
    21421 accagtgcgc aggaagccgc gaagaaaagt aatatcaagg tcggtttgat cgcttcggtg
    21481 aaccgccatg aaagccccga gcttgctgag caggtggtct ggctggcggc agagcatatg
    21541 aaggatggca tggtgggcat tgacctggct ggtaatgaag ccgagttcaa gtctgctcca
    21601 ttccgcgata ttttcaagga agcgaaacaa tccgggctgc atgtcaccat ccatgcgggt
    21661 gagtggggac ccgctgagaa tgtccgcgat gcgcttgtga atttcgatgc cgagcgtatc
    21721 ggtcacggtg tgcgcgtgat ggaagatgaa aatattgtgg cgctcgcgaa ggaacgcggc
    21781 gtgactttcg aagtgtgcgt aaccagcaat tatcagtccg gtgtggtcaa gtctttgccg
    21841 cagcacccgt tgccgcgtat gatggcggca ggcctgcatg tgaccgtcaa taccgatgat
    21901 cccagcgtgt cgcgcatcac gctctcgcat gaataccagc atgtctgcga ggatatgaag
    21961 gtttccttcg atgcgctgcg acagagtgtt ctgcatgcgg ttcaggcctc gtttacaacg
    22021 caggatgaaa aaacgaaact ggttgccgca ttgaaaaaag aaatgaagat gtaggttttt
    22081 ccctgcgcat atttgcgatg cacaggtttc aaatgatcgt tgtgatgaat cgtcccgagc
    22141 gtagcgaggg acgctgccat gtaaatccca ccacatagta aaaaggatat caaaggcgct
    22201 cctttgagtt cttccagatt gtggtgtgag cgtacgacaa acaatcttag tttgtcacac
    22261 gagaatcctc atgccaaagg agtatcctat gcaagacctg tttaagtccc gcgacgtatt
    22321 gaaagtggga aagaaggaat acgtctactt ccgccttgat gcgctggaga aggccgggct
    22381 ggtaaagctc agcaaactgc cttattccat ccgcatcgta ctggaggctg ccctccgcca
    22441 atgcaatgac aaggaaatca cgcaggagga tgtgaagaac atcgccgcgt ggtctcccaa
    22501 aggtgcgcgc cctggcattc cgt
//
LOCUS       DAOQJC010000061        21902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1073_length_21902_cov_7.918204, whole genome shotgun sequence.
ACCESSION   DAOQJC010000061 DAOQJC010000000
VERSION     DAOQJC010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 21902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21902
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1073_length_21902_cov_7.918204"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1890)
                     /locus_tag="PLE39_10725"
     CDS             complement(91..1890)
                     /locus_tag="PLE39_10725"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNE68847.1"
                     /translation="MDFSEKPKVSGSAIVVLIAVLLFGCIILGAFMIPTLKQMLQSNT
                     VAPAGATAVTDTTNATAAPPPGSVAIEISSSNTKEDWMNAVVEKFNAEQHKTADGNLI
                     FVTVKHVTSGGSQKDILNGKSQPTVWSPGDQSWVDEANTVWRDRTGQVLIPDQCAQTV
                     LAPIGFAMWKPMAQALGWPDTPISWDDIAALSADPKGWETLGHPEWGQFKFGHTHPDY
                     SNVGLLSMTALTYSTLGITEGLTADQVYSDAVVEAFTGVEQNTYHYGLQSRPLMQILA
                     QRGPEYLHAVTTSEAETLKTNKEFASTMRFPLVFIFPSKGTFWSEQPYCILNGEWVSD
                     EQKEAAATFRDYLLDKQQQEMAIDYYLRPIDTTIPLHAPLALDDGTDPRITMDTVPAL
                     QSPSADVSSAVKDVFHQTKKKSTIVLLLDISGSMEGEKIANAVSSSANFISRLDPNDE
                     IFLIVFGGGDGTVYSLGGGRAGDISEDLTTKLNGLYANGNTPLYDGVCTAIDKVNEVK
                     AEHDANNEKRLYGIVLLSDGQDTSSTRSQNQMFNCLPSGESVDGTKIFTIAYGGDADA
                     DLLLRIANHTNGKTFVGDPNSIETIYNAISAEQ"
     gene            complement(1904..2671)
                     /locus_tag="PLE39_10730"
     CDS             complement(1904..2671)
                     /locus_tag="PLE39_10730"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE68848.1"
                     /translation="MSTDYAARASIGMLKRIPAGVFRMGSKFHPREYPAKLTQVSDFQ
                     IAQTPVTVSQYEPFIDSGMIKEKKWWSDEGWEWFNGRGEGWGRKNREIPDGWKAQVNK
                     SFHPVTGVTFFETEAYCAWLGEMKGKAIRLPTEEEWEYAARGDDSRPFPWGEDFRATF
                     ANTLESEHSSTVEAASMAHDSSSFGVMDMCGNVQQWTSSVYTPLKDEVTPPGALQVAR
                     GGSFNDTLFGSRTSYRRGYPRGFFYPYLGFRIVVNNL"
     gene            complement(2700..3899)
                     /locus_tag="PLE39_10735"
     CDS             complement(2700..3899)
                     /locus_tag="PLE39_10735"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNE68849.1"
                     /translation="MSKENDAVILSAARTAVGKFQGGLSGFSATKLGAIAVQAAVERA
                     GIDPQNIEEVLMGNVVQAGNGQAPARQAAIFAGVPATVSATAINKVCGSGLKAAMMSA
                     QAIRAGDGDLFVAGGMESMSRAPYLVSGRSGELKFGNQALTDALLNDGLWDPFENWGM
                     GMAAEFIADEYEVTRAAMDEFALRSHLKAVEAQEKGMFSAEIVPVQIPGRKGEVTIFD
                     KDEGPRKDTSLETLAKLKPAFKADGKVTAGNASSMNDGAAAVVISSRAYAEKNNLKPL
                     ARIVGYAQAALEPKYLFAAPAHAIPKLLKKIDWKLSDVDLIEVNEAFAAQVLADGYAL
                     ADQGWDWDKVNVNGGAIAIGHPLGASGARVLTTLIYALKNRGLKRGVASLCLGGGEAV
                     AMAVEIE"
     gene            4002..4394
                     /locus_tag="PLE39_10740"
     CDS             4002..4394
                     /locus_tag="PLE39_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HNE68850.1"
                     /translation="MNELNSLYDLLGGEVGVRALVERFYDIMDSSPEAKDIRALHAAS
                     LKQSREKLFLFLTGWSGGPPLYIEKYGHPRLRQRHMPFSIGGIERDQWLWCMNQAVEE
                     MNILPAVKEHLRTRFAEVADFMRNREEG"
     gene            complement(4426..4842)
                     /locus_tag="PLE39_10745"
     CDS             complement(4426..4842)
                     /locus_tag="PLE39_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE68851.1"
                     /translation="MKYLLDTCVISELIAKTPNPNVVEFVDAIETDNIFLSAITIGEI
                     AKGIEKLPESKRKRELQTWLKEDLLVRFDDKVLPLDTEVLIQWGILSARLEGTGITLP
                     SMDSLIAATALTHNLTLITRNEGDFQNTGIEIINPW"
     gene            complement(4839..5087)
                     /locus_tag="PLE39_10750"
     CDS             complement(4839..5087)
                     /locus_tag="PLE39_10750"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNE68852.1"
                     /translation="MNSVWQIQDAKNKLSEVIARALQQGPQLITKHGEKTVVVISYIE
                     YEKLRKSQGKISEFFSASPLAGINLARDKSIPRKGLEL"
     gene            complement(5157..5831)
                     /locus_tag="PLE39_10755"
     CDS             complement(5157..5831)
                     /locus_tag="PLE39_10755"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNE68853.1"
                     /translation="MHKYSLALKSLFEKNANPAQAGPMQKYMRDQFEYLGIKTPQSAA
                     LMKGFVAEHGLPPLEKLDVIVRDLWSLPQREFQYAATGLIGKLEKKVEPEFITTIEYL
                     LVTKSWWDTVDTLASHAVGSQFKRFPEIRKTYLKKWRKSENFWLRRTTLLFQLGYKKE
                     TDFDLLCEIVGENLGSDEFFINKAIGWALRQYAHTNPSPVKKFVAATKELHPLSRREA
                     LKNIEE"
     gene            complement(5833..6321)
                     /locus_tag="PLE39_10760"
     CDS             complement(5833..6321)
                     /locus_tag="PLE39_10760"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNE68854.1"
                     /translation="MNHLWSPWRMSYIENHTKENGCVFCIAQAKADSEENLIAFRGKN
                     AYVILNRFPYTSGHLMVIPFSHTSNLEELDAQTRAEMMELASRCTTVLRNEYRTQSFN
                     MGINIGEAAGAGVLGHVHIHIVPRWVGDTNFMSSVGETRVLPETLEATYKRVRDAFQT
                     NP"
     gene            6434..7639
                     /gene="xseA"
                     /locus_tag="PLE39_10765"
     CDS             6434..7639
                     /gene="xseA"
                     /locus_tag="PLE39_10765"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNE68855.1"
                     /translation="MQPTLFSSVHLTVSQLTFHIRKQLEDDPTLQDVWVDGEISNLSK
                     PASGHIYFTLKDKNASLRCVIWKLDAGRLRVNLQDGMAVEVHGRITVYEPQGQYQLIV
                     NLVQPKGEGRLFQEYMRLKAMLESEGLFAADRKRLIPDRPQAIGIVTSATGAALRDML
                     NTLRRRQPLARVILAPSPVQGVEAPPALVNALNSLNRQKPDVILLARGGGSIEDLWAF
                     NEEQVVRAVAASKAPVICGVGHETDFTLCDFAADLRAPTPTAAAELATQTTLDDLHFQ
                     LSTISTRLTDLTSNLLAEQKAGLSALAGRLKYTSPERRIQSEYQHLDELSRRAWSAID
                     HRIQLQASLVDGMSKRLQSLNPMGILARGYAIITRKVDGRVVGKVADADSEMKVRVSD
                     GEFDVNRKS"
     gene            7668..7910
                     /gene="xseB"
                     /locus_tag="PLE39_10770"
     CDS             7668..7910
                     /gene="xseB"
                     /locus_tag="PLE39_10770"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNE68856.1"
                     /translation="MAKSKSSKSIEELSYEEALAELEQIVSTLEDEQNPLEDSMKLFE
                     RGQALVRQCGALLESAQLKVQKLAGESLIEFEEESE"
     gene            7907..8359
                     /locus_tag="PLE39_10775"
     CDS             7907..8359
                     /locus_tag="PLE39_10775"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HNE68857.1"
                     /translation="MNLAALLDNKVLIAVLAGWLIAQALKIPTEYFRSRRWMWAMFFA
                     AGGMPSSHSALMVSGTIAVGLYHGFDNPLFGLAVAVTMIIAHDAAGVRRQAGMHAERI
                     NVLFEELLKGHMWDENELKEVIGHTPLEVVGGILLGFFVAIMQWIIWP"
     assembly_gap    8408..8507
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8508..10069)
                     /gene="murJ"
                     /locus_tag="PLE39_10780"
     CDS             complement(<8508..10069)
                     /gene="murJ"
                     /locus_tag="PLE39_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNE68858.1"
                     /translation="MSETNGNANRQIARAAGTVMAAILLGQLAGLARGVIVARAFGAS
                     PELDAFFAANRVSETLFLLVAGGALGSAFIPMFTGLLAKNENDSAWRLASAIGNAVTL
                     TLSLLAVLVAVFAPQVVRYALAPGLSSDPQLFALTISLLRIQLISAVLFGIGGLIVGI
                     LNAHQVFLIPALTPAFYQLGIIFGALVLAPTLGIYGLAWGVVIGSVLYVALQIPTLAK
                     LAARQPQLATFHFSLGLHDANVRQVLMLMGPRLIGVAVVQLNFWVNTWLASQMASGSV
                     TGLYYGFSLMLMAQAAIAQSVAIAAMPTFSAQHALGQTDEMRASLASSLRGVILLALP
                     ASIGLMLLRQPLISMLYQRGKFDATDVQIVAWALLWYAAGIVGHSIMEILTRAFYAQH
                     DTRTPVIIGTIAMGLNVAFSILFAKLFTAIGWMPHGGLALANSFATALEATALFIVMR
                     KRLQGIEGRHILGGALPSTAAAVVMAFSIIGWLSAGKNLNAWILALIGVTIGGGVYFA
                     MLWILRVPELQY"
     gene            10106..11218
                     /locus_tag="PLE39_10785"
     CDS             10106..11218
                     /locus_tag="PLE39_10785"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNE68859.1"
                     /translation="MKKTVSLLFILILLTACTTTSEANPTPPVVGTVIESDFTNPLTV
                     VELGAGYGVRGDWFELYFTDPSSPLAAQETGGPDGPLVNAIDNARLSVDVAIYSLSLN
                     SVRDALLRAHDRGVRVRMVMESDNLDRSDPQKLKDAGIPILGDRREGLMHNKFVVIDN
                     LEVWLGSMNFTDSGAYKDNNNLMKIRSTKLAEDFTAEFEEMFVDDIFGPDLGNPTPNP
                     RVTIDGTNIDVYFAPEDHAQAALFDLVDSVAQASIYFMAYSFTADPIGNAIRSASAEG
                     LTVAGVMDADQIKSNLGTEYDPFKQAGLEVYEDNIPGLMHHKVMIIDDSIVVFGSYNF
                     TNSAETNNDETLLVIYNADIAAQFKAEFFRVFRSAQ"
     gene            11352..12176
                     /locus_tag="PLE39_10790"
     CDS             11352..12176
                     /locus_tag="PLE39_10790"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNE68860.1"
                     /translation="MKQFFRYLRPVLVLLALVMNACASAPVATTTDTGAAKVEASGVE
                     FTGVIESIDGNTWVVNGQTITVDPALVQGTYAVGDTVKIEASVAEDGSIVVTRLEAPS
                     SSDDNTNTGSNDNTSTGDNTNTGTGDNTNTGSGDNTNTGTGDNTNSGTDDNSNDSNSN
                     DDTDDDGDDNSNDGTDDNSNDGTDDNSNDGTDDNSNDDNSNDDGSDDNGNDDNSNDDG
                     SDDNSNDDNSNDDGDNSNDDNSNDDGDDDNSNDDNSNDDGDNSNDDNSNDGNTNGG"
     gene            12269..13774
                     /locus_tag="PLE39_10795"
     CDS             12269..13774
                     /locus_tag="PLE39_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68861.1"
                     /translation="MQKQTISKFLPLLLFVLFFIGMAAIQFATSDMPDNDGFYHIKLA
                     WLMRTEGLKPDFVWLPLSILSEKEFYDHHFLFHVALIPFTFGDLHVGAKWAAVTFSSL
                     AFLAVWYLFHRQRIPLAWLWALGLLGISDAFLYRMSITRAQSLSLGILALALAWILEG
                     KYKHLAVLSFIYVWAYDAFPLMMSLTVLHLLAVAMIERRLEYRLLLWSGIGVVLGVII
                     NPYFPANLVFTYRHLLPKLVEATSVSVGNEWYPYETAQLLENSLPALVAFVSGALALG
                     LTGRKMDVRTATGFLIALLFGLMLFQARRFVEYFPPFALIFAAFAWAPLFVDPEPASG
                     FQSRFSRSKWDELMANMPGLLLMLAVVVGMVKSIPAVQKQMQRSKSYELYADASAWLV
                     EHTAEGERVFQTDWDDFPRLFFYNTHNTYLIGLDPTYLQLYDAEMYDTWVSITRGKTP
                     NPSEIIATQFEARYVHSDLNHENFLRVAAEDPGLKEVYRDGQAVIFEVITP"
     gene            13961..14509
                     /locus_tag="PLE39_10800"
     CDS             13961..14509
                     /locus_tag="PLE39_10800"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE68862.1"
                     /translation="MPPNDLQKELDGLQKLDAQTIGAVYDQYFSEVYRYVRYRIGDSN
                     AAEDIASDVFVRLLEAVQNGQPPKTSLKGWLIGTASHLVTDHLRRKYRRPENELHESL
                     PDSGANVPSEVDQREQNQAVNNAYAQLTPEQQNVLALRFGQGYSLDETAAFMNKNVNA
                     VKALQFRALAALQREVGEVNYE"
     gene            14502..15836
                     /locus_tag="PLE39_10805"
     CDS             14502..15836
                     /locus_tag="PLE39_10805"
                     /inference="COORDINATES: protein motif:HMM:NF039823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HNE68863.1"
                     /translation="MNNIYDILEICLQELENGADMDALLARYPSFAGELRPILKASVE
                     ARTMAQEPSPEVVRRGRAKLLQHAAEMREAKLAPRKRVIPFFQRMAISFTMTALFLAS
                     GTGLVGASSSALPGENLYPVKLTWENVRLFFTFDHAAREVLEHAFGNERLHEISELLA
                     EGRHETIQFSGIFMDVNGLTYVSGIQVAILDTTNLPVDGLVNGAAVTVTGRTNAAGFV
                     DAASIILLPPGTVVPTGEPVKVEDNQNENINLNGNDNSGSEENENNGNDNVGVNENEN
                     ANENINDNENGNGNDNGNDNGDNNNDGGGDDDGNDNGGTDNENGNDNDGGDNGNDNAN
                     ENSNENENANENTNENDNGGSNDNDNVNDGVNDNTNDNSNDNTNDNVNDNSNDNDNSN
                     DNTNDDNNNDDNSNDDANSNDDDNTNSNGGSDPINARYVEAFLSRKDFTLNL"
     gene            15931..18327
                     /locus_tag="PLE39_10810"
     CDS             15931..18327
                     /locus_tag="PLE39_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNE68864.1"
                     /translation="MRTLPKSKWMRFLLVVIFLVGGYFISVFYDLPSVHAIPESLNQP
                     SVRITDRNGRLLYELIPEVGGRNTVLAVENIPQCMKDATIAVEDGNFYSNPGVDLTGI
                     VRALWINLSGGETLAGGSTITQQIARTLLLKDEVTERTVRRKLRETVLAWQLTQTYSK
                     DEILALYLNQIYYGGMAYGIEAAAQTYFGKPAVDLLLPECALLAGLPQTPGIYNPFTN
                     PDLALDRQKVVLGLMEKEGFITATQRIDAENSPLSYNAEPYPIEAPHFIWMIKDQLDE
                     LYLSGQLSVEQSLIIRTTLDLDMQNLAEEIVKRRIELFKPLEGQTNFNVKNAALVVLD
                     PHTGEILTLVGSADYFDASINGALNMAITRRQSGSAFKPIIYAAALDPTRMDAITAAT
                     PILDVATTFTTKDGQPYKPVNYDGREHGFVPVRQALASSLNVPAVLTLQNVGIENAIS
                     LANQLGIRSLSDPRNYDLSLALGGGSMNLLELSGAYAALANEGNVTGLFSVLEIRDAD
                     GNLLYEPERPAAHRVVDARVAWLISDILSDDQSRALGFGINSVLKIDRTAAVKTGTTT
                     NFHDNWTIGYTPDLLVGVWVGNSNQEAMHNVTGLTGAAPIWHETMRSILQGRPDKMFS
                     QPAGLTQINVCSLSGLLPTEACQYTKNEWFIEGTQPTAYDAFYQRLDTGQVVLSLPVE
                     AQAWARSQGLPLLDLANEPNFEADFLTLTSPTDHTTYRITPNLDLSAQQLAFSAPTVP
                     GLTQMNFFVDGASFATLNSPPFQVWWPLSLGQHRFWVEGIMSTGELVKSTIVTITVVE
                     "
     gene            18372..18773
                     /locus_tag="PLE39_10815"
     CDS             18372..18773
                     /locus_tag="PLE39_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE68865.1"
                     /translation="MTKIMIVDDDPEATSILSSLLKIEGYIPISVNDSTLAIQKANAV
                     QPDLFLLDLMMPVLDGFKLCRLLREERIFLRTPIIIVTALSSADSRVVAFGAGANDYI
                     TKPYLPHELVLKIREQLERARAGLIDPFDGL"
     gene            complement(18800..19651)
                     /locus_tag="PLE39_10820"
     CDS             complement(18800..19651)
                     /locus_tag="PLE39_10820"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNE68866.1"
                     /translation="MTAYIFAWYLFLFIPLLFFAWATRRKWLGLTVTSSILIIVFTYL
                     PLYLPNRPASSAEGGFSVKIMSYNLHGIPEIDGIVEVIRRENPDVLLVQECSPALCSP
                     LYHGLDDLYPYKDMVIKDFGQAAYSRYPMKRTSAEANQGRTQKLIIKAPQGPAAVWNI
                     HPIPPFLIPPQEYDAQMSALVADIAREKGPLIVAGDLNATDQSEAYRKINQYLIDGYW
                     ETGWGVGFSYPAPPYTFMDSGLQMGPIWRIDHIFHSSEFTATDAYIVKDAGGSDHFPI
                     VVVLSLK"
     gene            19878..20216
                     /locus_tag="PLE39_10825"
     CDS             19878..20216
                     /locus_tag="PLE39_10825"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNE68867.1"
                     /translation="MAPTNSKTVWSSEEGDLRKKEAASASSHSLPPQQQTIYLHRESK
                     GRGGKGVTLVKGLVLSEADLTALAKKLKQACGSGGTIKDGVIEIQGEQREKIAEVLRS
                     LGYKVKIAGG"
     gene            complement(20252..21478)
                     /locus_tag="PLE39_10830"
     CDS             complement(20252..21478)
                     /locus_tag="PLE39_10830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE68868.1"
                     /translation="MAETPNFDKASLLSLAFKGLAENDLREMATLTRLCTYPADHILC
                     REGAYEEIFYIIADGNAVITKQISEEEGERVLRIAGKGDLVGEMALIQNSPRSATVRA
                     TTELTVLEMGKKDFETMLSRSPSMAINIIRTTLDRILENDQMAILDLQKNNKVLRQLD
                     RNKMEFIQVAAHELRTPLTIIKGYVNVLGSFPELQENAALKEVMEGIVKGSERMHEVV
                     NTMLDVTRIENEQLRISPAPVPLKRILHELSSDFRKAAEDRNIEIIVEQDEDTPAINA
                     DPSLIQKALYHLIVNAIKFTPDGGKITLRSYPVLMENHAPGVELSIQDTGIGLDAEHH
                     ELVFEKFYQVGNVSIHSSGKTSFKGGGPGAGLAIVRGVARAHGGKVWVESKGHDEVNF
                     PGCTFHLQLPIDPPRH"
     gene            complement(21661..21855)
                     /locus_tag="PLE39_10835"
     CDS             complement(21661..21855)
                     /locus_tag="PLE39_10835"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNE68869.1"
                     /translation="MTTQRQALRRSRSNRVVAGVCGGLAEFFGVSTFWFRLAFLIALI
                     PGGVPGILVYLAMVFIVPSE"
BASE COUNT         5136 a         5554 c         5647 g         5465 t
ORIGIN      
        1 gacgatgaat gtggtgacaa tgctcatgat caggatgagc cagccgtatg gatcgagcag
       61 gaaccaatat ggacggggtt gctgcatgtc ttattgctcc gcggaaatgg cgttgtaaat
      121 ggtctcaatg ctgttcgggt cgccgacaaa ggtctttccg ttggtgtggt tggcaatgcg
      181 taacagaagg tcggcgtcgg catctccacc gtaggcgatg gtgaagatct tcgtgccatc
      241 cacgctttct ccacttggca gacagttgaa catttgattc tgcgagcggg tgctggaggt
      301 atcctgtcca tcggatagca gcacaatgcc gtacaggcgt ttctcgttgt tcgcgtcgtg
      361 ttcagccttg acttcgttca ctttgtcgat ggctgtgcat accccatcat agagcggtgt
      421 gttgccgttc gcatacagac cattgagctt ggtcgtcagg tcttcgctga tatcgcctgc
      481 gcgcccgcct cccaatgagt agactgtccc atcgcccccg ccaaaaacaa tgaggaagat
      541 ctcatcgttc gggtcgagcc tgctgatgaa gtttgccgag ctggagaccg cattggcgat
      601 cttctcacct tccatgctgc ctgagatgtc gagcaacagc acaatggtgg atttcttctt
      661 tgtttggtgg aagacgtctt tcactgccga agacacatcc gcgcttgggc tttgcagagc
      721 aggcacggtg tccatggtga tgcgggggtc ggtcccatca tcgagggcaa gcggcgcatg
      781 caatggaatc gtcgtgtcga tcgggcgcag gtagtagtca attgccattt cctgctgctg
      841 tttatcgagc aggtaatcgc ggaaggtggc tgccgcctct ttttgttcgt cacttaccca
      901 ttccccgtta aggatgcagt acggttgttc gctccagaac gtgcctttgg acggaaagat
      961 aaagaccaac ggaaagcgca tggtggatgc gaattccttg tttgtcttga gtgtttccgc
     1021 ttcgctggtg gtgaccgcat gcaggtactc aggtccgcgc tgggcgagga tctgcatcaa
     1081 tgggcggctt tgcaaaccat agtggtaggt gttctgttcc acgcctgtga atgcctcaac
     1141 gaccgcatct gaatacacct gatctgcggt gagcccttcg gtgatcccga gcgtggaata
     1201 ggtcagggcg gtcatgctca acagaccgac attggaataa tcgggatgtg tgtggccgaa
     1261 cttgaattgt ccccactcag gatgtccaag cgtttcccat ccctttgggt ctgctgaaag
     1321 cgcggcgata tcgtcccagc tgatgggggt atcgggccag ccgagcgcct gtgccatggg
     1381 tttccacatc gcaaagccga tcggggccag cacggtctgt gcacattgat ctgggatcag
     1441 cacctgcccg gtgcggtcac gccagaccgt gttcgcttca tccacccatg attgatcgcc
     1501 cgggctccat accgtcggct gactctttcc atttaggata tctttctgcg atccacctga
     1561 agtgacatgt ttcactgtaa cgaagatcag gttgccgtcc gcggttttat gctgttccgc
     1621 gttgaatttt tcaacgacag cgttcatcca atcttctttc gtattggatg atgagatctc
     1681 gatcgcaacc gacccgggtg ggggagcggc cgttgcattc gttgtgtctg tcactgcggt
     1741 tgcgcctgca ggcgcaaccg tattgctttg caacatctgc ttgagggtgg ggatcataaa
     1801 cgcgccgaga atgatacaac cgaataacag cacggcaata agtaccacga tcgcgcttcc
     1861 tgaaactttt ggtttttctg agaagtccat gcttcctcca aatttataag ttgttcacaa
     1921 cgatgcgaaa ccccaggtac ggataaaaga agccgcgcgg atatccgcgg cggtaggagg
     1981 ttcgcgagcc aaagagggtg tcgttgaatg aacctccacg cgccacttgc aatgctcctg
     2041 gcggggtaac ctcgtctttc aacggtgtgt aaaccgatga tgtccactgc tggacatttc
     2101 cgcacatatc catcacgcca aaggaacttg aatcgtgtgc catgcttgcg gcttccacgg
     2161 tggaactgtg ctcactttca agggtgtttg caaaagtggc acgaaaatcc tcgccccatg
     2221 ggaagggacg tgaatcatca ccacgtgcgg catattccca ttcctcttcg gtcggcaggc
     2281 ggattgcctt tcccttcatt tcgcccaacc aggcacaata ggcttccgtt tcaaaaaaag
     2341 tgacccccgt caccgggtga aaggatttat ttacctgcgc tttccatcca tctggtattt
     2401 ccctgttctt gcgcccccag ccttcgccgc ggccgttgaa ccactcccag ccttcatcgc
     2461 tccaccattt tttttccttg atcataccgc tgtcaatgaa tggttcatac tgactcacag
     2521 ttactggagt ctgggcgatt tgaaaatcgg atacttgcgt aagttttgct ggatactcac
     2581 gcggatggaa cttacttccc atgcggaaca ctcccgctgg gattcgtttc aacattccaa
     2641 tggaagccct tgccgcataa tcggtagaca tcgccaatca ccaatgacca atcgctgctt
     2701 tactcaatct ccacagccat ggctaccgct tcgccgccgc cgaggcagag cgatgcgact
     2761 ccgcgtttca ggccgcgatt tttcagggca tagatgagag tcgtcaacac acgggcaccg
     2821 ctggcgccga gtgggtgacc gatggcaatt gcgccgccat tcacgttgac cttatcccag
     2881 tcccagccct gatcggcgag agcgtaacca tctgcaagca cctgtgccgc aaaggcttca
     2941 ttcacttcga tgaggtcaac gtcgctgagt ttccagtcaa ttttcttgag caactttgga
     3001 atggcatgag caggagcggc aaacaaatat ttgggttcca atgccgcctg cgcgtagcca
     3061 acaattcgcg cgagaggttt gaggttgttt ttttctgcat aagcacgtga cgagatcaca
     3121 actgccgccg cgccatcatt catggaagag gcgttgcctg cggtcacttt tccatcggct
     3181 ttgaaggcag gtttgagttt tgcaagtgtt tccagtgaag tgtccttgcg ggggccttcg
     3241 tccttgtcga agatcgtcac ttcccctttt cggccaggga tttgcacagg aacgatctcg
     3301 gcgctgaaca tgcccttttc ttgagcctca acagctttca ggtgagagcg aagcgcaaat
     3361 tcatccatcg cggcgcgagt cacttcgtat tcatccgcaa tgaattcggc ggccatcccc
     3421 attccccaat tttcaaaagg atcccaaagg ccgtcgttga gcagggcatc ggtgagggcc
     3481 tgattgccaa atttgagttc acccgaacgt ccgctgacaa gatacggcgc acgactcatc
     3541 gactccattc cgcctgccac gaacaggtct ccatctccag cgcggatggc ttgcgccgac
     3601 atcatcgccg ccttgaggcc tgatccacaa actttattaa tagccgtggc gctgacagtg
     3661 gctggtaccc ccgcaaaaat agccgcctga cgcgcaggcg cctgaccatt gccggcttgg
     3721 acgacattgc ccatcaggac ttcctcgatg ttttgcgggt caatacctgc acgctccact
     3781 gctgcctgta cggcaatggc accgagtttt gttgcggaaa agccgcttaa cccaccctga
     3841 aacttgccaa cggcggttcg tgctgcactt agaatcactg cgtcattttc tttgctcatg
     3901 ggttctcctg ttggtacgcg gtcgttattt tctattttct cataacaatg atgaactgtc
     3961 attcccaact tgtttatgaa acttatatta caatttcacc catgaatgaa cttaactccc
     4021 tctatgacct gctcggcggc gaagtgggcg tccgcgcttt ggttgaacga ttttacgaca
     4081 tcatggattc ttcgcccgag gcgaaggata tccgtgcctt gcacgcggcg agcttgaaac
     4141 aatcgcgcga aaaacttttt ctctttctta caggctggtc gggcgggccg ccgctttaca
     4201 ttgagaaata tggtcatccc cgtttacgtc aacgccatat gccattttcc atcggcggca
     4261 ttgagcgtga ccagtggctg tggtgcatga atcaagccgt tgaagagatg aatatcctcc
     4321 ctgctgtgaa ggaacatctc agaacccgtt tcgccgaagt ggcggatttt atgcgaaata
     4381 gagaagaagg atagggtgtt gagctaaaag gtcacgtaga ctcactcacc acgggttaat
     4441 aatctcaatc cccgtatttt gaaagtctcc ctcatttcgt gtgataaggg ttaagttgtg
     4501 ggttaaggct gtggcggcaa tcaaggagtc catggaggga agtgttatgc cagtgccttc
     4561 aagtcgtgca gacaggattc cccattgaat taaaacttct gtatcaagag gtaagacttt
     4621 atcatcaaaa cgaacaagca aatcttcctt gagccatgtt tgtaattctc gttttcgttt
     4681 tgattcggga agcttttcaa tacccttggc tatttctcca attgtgattg cactcaagaa
     4741 tatattgtct gtctcaattg cgtcaacgaa ttctacaaca tttggattgg gtgttttggc
     4801 aattaattcc gaaatgacgc aagtgtcaag cagatatttc acagctcaag tccttttcgt
     4861 ggaatggatt tatcccgggc aaggttgatt ccagccaagg gagaggcgct gaaaaactcg
     4921 gagattttcc cttgagattt gcgcaatttc tcatattcaa tataagaaat aactacaacg
     4981 gttttttcgc catgtttggt tattaattgc ggaccctgtt gtagggctcg agcaattact
     5041 tcactgagtt tgttcttggc atcttgaatt tgccataccg aattcataat ctctccttta
     5101 tgtctagtct gtctagattt tacgacaggc aaggtgtgct gtcaatcttc atacttttac
     5161 tcctcgatat tcttcaatgc ctcgcgccga cttaatggat gcagttcctt tgtagcagcg
     5221 acaaactttt ttacaggaga cgggttggtg tgtgcgtact ggcgtaatgc ccagccaatg
     5281 gctttgttga tgaagaattc gtccgaaccg aggttttccc cgacgatctc gcacagcagg
     5341 tcgaaatctg tttctttttt ataacctagt tggaaaagca gggtcgtacg ccgcagccaa
     5401 aagttctccg attttcgcca ctttttcagg tatgttttgc ggatttctgg aaagcgtttg
     5461 aactgagacc ccactgcatg acttgcaagt gtatccactg tatcccacca cgattttgtg
     5521 accagcagat attcaatggt cgtaatgaat tcaggttcaa ccttcttttc gagtttgcct
     5581 atcaaccccg tagcggcata ttgaaattct cgctggggta gtgaccacag gtcacgcacg
     5641 atcacatcca atttttccag cggggggagt ccgtgttcgg caacgaatcc cttcatcagc
     5701 gccgcacttt gcggcgtttt gattccgaga tattcgaatt ggtctcgcat gtatttttgc
     5761 atgggccctg cctgtgcagg gttggcattt ttctcaaaaa gggatttcaa agcaagggag
     5821 tatttgtgca ttttaggggt ttgtctggaa ggcatcccgc acgcgtttgt aggtagcctc
     5881 aagcgtttcg ggcagtaccc gtgtttcgcc cacagatgac ataaagtttg tgtcgcccac
     5941 ccagcgcggc acaatatgaa tgtggacatg tcccagtacg cccgctcccg ccgcttcacc
     6001 gatattgatg cccatattga acgattgggt ccgatattcg ttgcgtagaa ccgtggtaca
     6061 gcgtgaggcc agttccatca tttcggcgcg ggtttgcgca tcaagttctt ccaggttgga
     6121 tgtgtgtgaa aagggaatga ccatcaaatg tccgctggtg taggggaagc ggttgaggat
     6181 cacgtacgcg ttttttccgc ggaaggcgat gaggttttct tcgctgtccg ctttggcttg
     6241 tgcaatgcaa aagacacagc cgttctcttt tgtgtggttt tcaatgtatg acatgcgcca
     6301 gggtgaccag agatgattca tgggagtatt aagttgaatt ataaaggatg acagatgaaa
     6361 gaagaaacct gctttttgat gtttttcttt ccatccttcc atattcatcc tttttaagtt
     6421 atcctcgcca ctcatgcaac cgaccttgtt ttcttccgtg catctcacgg tttcccagct
     6481 caccttccat attcgaaaac aattggagga tgatcccacc ctgcaggatg tttgggtgga
     6541 tggtgaaatt tcaaaccttt cgaaacctgc ctcgggacat atctatttca cgctgaaaga
     6601 taagaatgcc tcacttcgtt gtgtgatctg gaaactggat gcaggacgct tacgggtcaa
     6661 cttgcaagat ggcatggccg tggaagttca cggcaggatc acggtgtatg aaccgcaggg
     6721 acagtatcag ctcattgtca accttgtgca gcccaagggt gaaggcaggt tgtttcagga
     6781 atacatgcgg ctcaaagcca tgctcgaatc tgaagggttg tttgccgctg accgcaaacg
     6841 cttgatccct gacaggccgc aagccattgg catcgttacc tcagcaacag gcgccgcctt
     6901 gcgcgatatg ctcaacacac tgcgccgacg ccagccactg gcacgtgtca ttttggctcc
     6961 ctcgcccgtg caaggcgtgg aggcgccgcc tgccctggtc aatgcgctga attctctcaa
     7021 tcgacagaag cctgatgtca ttttgcttgc acgcggtggc ggttccatcg aagatctgtg
     7081 ggctttcaat gaagaacagg ttgtccgcgc cgtcgcggct tcaaaagcac ctgtgatttg
     7141 tggggtaggt catgaaactg atttcacttt gtgcgatttc gccgctgacc ttcgtgcccc
     7201 gactccgacc gctgctgcag aacttgccac ccaaacaacg ctggacgatc tccattttca
     7261 actttccacg atttccaccc gcctcaccga cttaacctcg aacctgcttg ccgaacaaaa
     7321 ggcaggtctc tccgctttgg caggtcgttt gaaatacacc tctcctgagc gccgtatcca
     7381 atctgaatat caacacctcg atgaactctc ccgccgtgca tggtctgcca tcgatcaccg
     7441 catccaattg caggcctctc tcgtagatgg aatgtcaaaa cgcttgcaat cgttgaaccc
     7501 catgggaatc ctcgcgcgtg gatatgctat catcacgcgg aaagtggatg gcagagtggt
     7561 cggcaaggtg gctgatgccg atagtgaaat gaaagtgcgt gtcagcgacg gagaattcga
     7621 cgtaaatcgc aagtcgtagg atcaaacatt attttggagt ggttttcatg gctaaatcaa
     7681 aatcgtcaaa atccatcgaa gaattgtcat atgaagaagc gctggccgag cttgaacaga
     7741 tcgtttcaac gctcgaagat gagcagaatc cgcttgaaga ttcgatgaag ctttttgaac
     7801 gggggcaggc tttggtcagg cagtgcggcg cattgcttga atctgcccag ttgaaggtgc
     7861 agaaactggc tggcgaatcc ttgatcgagt tcgaggaaga atccgaatga acctggcggc
     7921 gcttttggat aacaaagtgt tgatcgctgt gctggcgggt tggttgatcg cgcaggcact
     7981 gaagattccc accgagtatt ttcgctctcg tcgttggatg tgggccatgt tttttgcggc
     8041 aggcggaatg ccatcctctc actcggcctt gatggtttca gggaccattg cggttggttt
     8101 gtatcatggg tttgataacc cgctttttgg gctggccgtg gctgtgacga tgatcatcgc
     8161 tcatgatgcg gcgggggtcc gcaggcaggc ggggatgcac gccgaacgca ttaacgtgtt
     8221 gttcgaagaa ctgctcaagg ggcacatgtg ggatgaaaat gaattgaagg aagtcatcgg
     8281 ccatactccg ctggaagtgg ttggcggaat tttacttggc ttctttgtag ccatcatgca
     8341 gtggatcatc tggccgtaga taaatcaaat ccccgcttgt ttgtttactt ttatcgagcg
     8401 tccttctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg tactgcaatt
     8521 caggcacacg cagtatccat aacatggcga agtaaacccc gcctccgatc gtcacgccga
     8581 tgagggcgag tatccacgcg tttaagtttt ttcctgctga gagccagcca atgatggaga
     8641 aggccatcac tactgccgcc gctgttgagg ggagcgctcc acccaaaatg tgccgacctt
     8701 caatgccttg tagtcgtttg cgcatcacga tgaaaagagc ggttgcctca agagccgttg
     8761 caaatgaatt cgccagggcc agcccgccgt gcggcatcca gccgatggcc gtgaacagtt
     8821 ttgcgaacag gatgctgaag gctacattca accccattgc gattgtgccg atgatgacgg
     8881 gggtacgggt gtcatgttgg gcgtaaaagg cgcgggtcaa gatctccatg atggaatggc
     8941 caacgatccc cgccgcgtac cagagcaaag cccaggcgac gatctgcaca tcggtggcat
     9001 cgaactttcc gcgttgataa agcattgaaa tgagcggctg ccgcaacagc atcaacccga
     9061 tactggcggg cagggctagc agaatgacgc cgcgcagaga ggaggccaat gacgcacgca
     9121 tctcgtccgt ttggccgagg gcatgttgcg ctgaaaaggt tggcatggct gcaatggcca
     9181 ctgattgcgc aatggctgcc tgtgccataa gcatgagcga aaacccataa tacaaccctg
     9241 tgacactgcc actggccatt tgagaggcca gccatgtatt tacccaaaaa ttcaattgca
     9301 ccacagccac gccgatcaag cgcggcccca tgagcatcaa aacctggcgg acgtttgcat
     9361 catgcaggcc aagagagaag tgaaaggtgg cgagttgtgg ttggcgggcg gcaagttttg
     9421 ccagagttgg gatttgaagt gcaacataca acacagagcc gatgaccacg ccccatgcaa
     9481 gcccgtagat tcccagcgtt ggagcgagga ccagtgcgcc aaaaataatt ccgagttgat
     9541 aaaaagcagg cgtgagtgca gggatcaaaa agacctgatg cgcgttcaaa atgccgacga
     9601 tcaatccgcc gatgccaaag agcacggcag atatcaactg gatgcgcagc agcgaaatgg
     9661 tgagagcgaa aagttgcggg tcactggaaa ggccaggggc cagtgcgtag cggaccactt
     9721 gcggggcgaa gactgccact aaaacagcaa gcaggctgag ggtcaatgtg accgcatttc
     9781 caatggcaga ggccagcctc caggctgagt cattttcgtt tttggcgagc agtcccgtaa
     9841 acatcggaat gaaggctgat ccgagcgcac cgcccgctac aagcaggaac aatgtttcac
     9901 tgacacgatt ggctgcaaaa aaagcatcaa gctcgggcga ggctccaaac gcgcgagcca
     9961 cgatgacgcc gcgggcaagc cctgcaagct gcccaagtaa aatggcagcc atcaccgtgc
    10021 cagccgcgcg cgcgatctga cgattggcat ttccgtttgt ttctgacata gggtggcggg
    10081 attataatcg ttttgggaga attccatgaa aaagaccgtg agcctgcttt ttatcttgat
    10141 cttattgaca gcatgtacca ccacctcaga agcgaatccg actccgcctg tagtgggtac
    10201 ggtgatcgaa tcagatttca ccaacccgtt gaccgtggtt gagcttggtg cagggtatgg
    10261 cgtgcgcggc gactggttcg agttgtattt caccgacccg agcagcccgc ttgccgcaca
    10321 ggaaacaggc ggccctgatg gtccgcttgt gaacgccatt gacaatgccc gtttgagcgt
    10381 ggatgtggca atttacagtt tgagtctaaa tagtgttcgt gatgcattgc tgcgcgcgca
    10441 cgaccgcggc gtacgcgtac gcatggtgat ggaaagcgat aaccttgacc gttctgaccc
    10501 gcagaaattg aaagatgctg ggattcccat tttgggtgat cgccgcgaag gcttgatgca
    10561 caataaattt gtggtgatcg ataatttgga ggtttggttg ggctcgatga attttaccga
    10621 ctctggcgcc tacaaagaca ataacaacct gatgaagatc cgctctacga aacttgccga
    10681 agatttcact gctgaattcg aagagatgtt cgtggatgat atttttggcc ccgatctggg
    10741 taaccccacc ccgaatccgc gggtaacgat tgatggtacg aatattgatg tgtattttgc
    10801 gcccgaagac catgcacagg cagccttgtt cgacctggtg gattctgtgg cacaggccag
    10861 catttacttt atggcatatt cctttactgc cgacccgatc ggcaatgcca tacgctctgc
    10921 ctctgccgaa gggttgaccg tggcaggggt gatggatgcc gaccagatca aatccaacct
    10981 gggcactgag tatgacccat tcaaacaggc aggccttgag gtgtatgaag ataacatccc
    11041 tggcttgatg caccacaagg taatgatcat tgatgacagc atcgtagtgt ttggctcgta
    11101 taattttacg aacagcgccg agaccaataa cgatgaaaca cttttggtca tctataatgc
    11161 cgatattgcc gcccaattca aggctgagtt cttcagggtg ttccgttcgg cacaatagag
    11221 aagaattgac tggtgtattc tttcacaaaa gaaaaacgag ttttgaaaat tgcacggaga
    11281 aagccgtaac cttttcgcct ctttgacgac tactcgtatg taaccaaatc aaacaaaaag
    11341 aggtgaaaat catgaaacaa ttcttccgct atctcaggcc cgttctggtc ctccttgccc
    11401 tggtaatgaa tgcttgtgca agtgctcctg tcgccaccac aaccgatacc ggcgccgcca
    11461 aggttgaggc atctggtgtt gagttcacag gtgtgatcga atccattgat ggaaatacct
    11521 gggttgtcaa cggccagacc atcacagttg accccgcatt ggtgcagggt acctacgcag
    11581 tcggcgacac cgtcaagatc gaagcctccg tggctgaaga tggttccatt gtggtgaccc
    11641 gccttgaagc cccgtcatct tctgatgaca ataccaacac tggcagcaac gataacacca
    11701 gcactggcga caataccaac actggcaccg gcgacaacac caatactggt tctggcgata
    11761 acaccaacac cggcacaggt gacaacacca acagcggtac cgacgacaac tcgaatgaca
    11821 gcaattcgaa tgacgatact gatgatgatg gtgatgacaa cagcaacgac ggcaccgacg
    11881 acaattccaa cgacggtacc gatgacaaca gcaatgacgg caccgacgat aacagcaacg
    11941 atgacaactc caacgacgac ggttccgatg acaacggcaa cgacgacaac tccaacgacg
    12001 atggttccga tgacaacagc aatgacgaca actccaacga cgatggcgac aacagcaacg
    12061 acgacaactc caacgacgat ggcgatgacg acaacagcaa tgatgacaac tccaacgacg
    12121 atggcgacaa cagcaacgac gacaactcca acgacggcaa caccaacggc ggctaatcgt
    12181 ctcaacgact gaaattcaaa tcgaaaggct cgcctggagc aaaatccttc aggcgagccg
    12241 ctcttattcc aacgggccag gtatttcaat gcaaaagcaa accatttcca aatttcttcc
    12301 cctgctatta ttcgttcttt ttttcatcgg catggctgca attcaatttg caacctccga
    12361 tatgcccgat aatgatggct tttatcacat caaactggcc tggctcatgc gcactgaagg
    12421 actgaaaccc gattttgtct ggctgccctt gagcattctc agcgaaaaag agttttacga
    12481 tcaccacttt ctgttccatg ttgccttgat ccccttcact tttggcgatt tgcatgtggg
    12541 tgccaaatgg gcggctgtca ctttttcaag cctggctttc ttggcggtct ggtacttgtt
    12601 tcatcgtcag cgaattccgc tggcctggct gtgggccctg gggttgctcg gtatttccga
    12661 cgcattttta taccgcatga gcatcacccg cgcccaatcc ctctctcttg gaattctggc
    12721 gcttgcattg gcctggattt tggaaggcaa gtataaacac cttgcagtgc tctcattcat
    12781 ttacgtttgg gcatacgacg ctttccccct gatgatgtca ttgaccgtgt tgcatctgct
    12841 tgccgttgcc atgatcgagc gccgcttgga ataccgcctg ctgttgtggt cagggattgg
    12901 agtggttctg ggtgtgatca tcaatccata ttttcccgcc aatctggttt ttacctaccg
    12961 ccacctgctg cccaaattgg ttgaagccac ttccgtcagt gttggcaacg aatggtatcc
    13021 ctatgaaacg gcgcaactgc ttgaaaactc cctgcctgca ctggtggcct ttgtcagcgg
    13081 cgcactcgcc cttgggttaa ctggccgcaa aatggatgtc cgaaccgcga ccggcttttt
    13141 gatcgcattg ctctttggat tgatgctttt ccaggcacgc cgcttcgtgg aatattttcc
    13201 gcccttcgcc ttgatctttg ccgcatttgc ctgggcgcct ctttttgtgg accctgagcc
    13261 tgcttctggt ttccaatcca gattctcccg ttcgaagtgg gacgagctca tggccaacat
    13321 gcctggactt ttgcttatgc tggcggtcgt agtgggtatg gtgaaatcca tccctgccgt
    13381 acaaaagcag atgcagcgtt ccaaatccta tgaattgtac gccgatgcat ctgcctggct
    13441 ggtggaacac actgctgaag gggagcgcgt tttccagaca gattgggatg attttccgcg
    13501 cttgttcttt tataataccc ataacaccta cctgatcggc ctcgatccga cctatctgca
    13561 attgtacgat gcagaaatgt acgatacctg ggtaagcatt acccgcggca agacccccaa
    13621 tccatcggag atcattgcca cacaatttga agcccgctac gttcacagtg acttgaacca
    13681 cgaaaatttt ctgcgagtgg cggccgaaga cccagggctg aaggaagtgt atcgtgatgg
    13741 tcaggcggtg atttttgagg tgataacgcc ttgagtttgt gaaggatgat gatgatcaag
    13801 caagttgcag ttgatggaga aagctttgaa atgaaaagga catccccacg ccttttcctg
    13861 atagccccgt gctgcgggag cgttacagaa tacgatgccg ccctatctga tggatctgcc
    13921 atgtcggatt tgatttcatg gctcccggga gagagatatt atgcctccca atgacctgca
    13981 aaaagaattg gatggactgc aaaaactgga tgcgcagacg atcggcgcgg tgtacgacca
    14041 gtatttttcc gaggtctatc gctacgtccg ctaccgcatc ggagattcaa atgcggctga
    14101 agatatcgca agtgatgtat ttgtgcgtct tctggaggcc gttcaaaatg gacagccccc
    14161 gaagacaagt ttgaaaggct ggttgatcgg tacagcctcc catttggtca ccgaccatct
    14221 gcggcggaaa tatcgccgcc cggaaaacga actccacgaa tcccttccag attctggcgc
    14281 aaatgtgcct tctgaagtgg atcaacggga acagaatcag gcagtgaaca acgcgtacgc
    14341 gcaattaact cctgagcagc agaatgtgct tgccctgcgt tttggacagg gatactcttt
    14401 agacgagact gcggccttta tgaataaaaa cgtcaacgcg gtcaaggctc tccagttccg
    14461 tgcgctggcc gctcttcagc gcgaggttgg tgaggtgaat tatgaataac atatacgaca
    14521 ttttggaaat ttgccttcag gaacttgaaa atggtgcaga tatggatgcc cttctcgcgc
    14581 gctatcccag ctttgctggt gaactgcgcc cgatcttaaa agcatccgtc gaagcacgga
    14641 ccatggctca agaaccctcg cccgaggtgg ttcgtcgcgg gcgtgcaaaa ttgttgcagc
    14701 atgctgctga aatgcgcgaa gcaaagcttg caccacgcaa acgagtcatt cccttctttc
    14761 agcgcatggc gatctcattt acgatgaccg ccctgttcct tgctagcggc actggtttgg
    14821 tgggtgcctc ttcttcggcc ctgcccgggg aaaacctgta ccccgtcaaa ctcacctggg
    14881 agaatgtgcg tctcttcttt acctttgatc atgcggcccg ggaagtgctt gaacatgcct
    14941 ttggaaatga acgcctgcat gagatcagtg aacttcttgc cgaaggcagg catgaaacca
    15001 ttcagttttc aggcatcttt atggatgtca atggcctgac ctatgtttcg ggtattcagg
    15061 tggcgatcct ggacacgacc aatcttcccg tggacgggct tgtaaacggt gcagccgtca
    15121 cagtgaccgg gcgcaccaac gcggcaggtt ttgtggatgc ggcgtccatc atacttctgc
    15181 ctccagggac agtcgttccc acgggtgagc cggtcaaggt ggaagacaat cagaatgaaa
    15241 atatcaacct gaacggcaac gataacagcg gttcggaaga aaatgagaac aacggcaatg
    15301 ataacgttgg cgttaatgag aacgaaaacg ccaacgaaaa tatcaatgac aatgaaaacg
    15361 gcaatggcaa cgataatggt aacgacaatg gcgataataa caatgacggt ggcggcgatg
    15421 acgacggcaa cgataatggc ggcactgata acgaaaacgg caatgacaac gacggcggcg
    15481 acaatggtaa tgacaatgcc aacgaaaaca gcaatgaaaa tgaaaacgcc aacgaaaaca
    15541 ccaatgagaa cgataatggc ggcagcaacg ataatgacaa tgtaaatgat ggcgttaacg
    15601 ataacaccaa cgataacagc aatgacaaca cgaacgacaa cgttaatgac aacagcaatg
    15661 acaacgataa cagcaatgac aacaccaatg acgacaacaa caatgatgac aatagcaacg
    15721 atgatgccaa cagcaatgac gacgataata cgaacagcaa cggcggatca gacccgataa
    15781 atgcccgtta cgtagaggct tttctttctc gaaaagattt cacgctgaat ttgtagccga
    15841 tgggttgtcc ctgggcccgc atggattgcg ggctcagttt tctctatcgt gtgatgtaga
    15901 tttttcccaa aagttggcga tcgtcatttt atgcgtaccc tgccaaagtc caagtggatg
    15961 cgatttcttc tggtcgtgat ttttcttgtg ggggggtatt tcatttccgt tttttacgac
    16021 ctgccttcgg tgcatgccat ccctgaaagc ctgaaccagc ccagtgtccg cattacagat
    16081 aggaatggac gcttgcttta tgagcttatt cccgaagtag gcgggcggaa cactgttctc
    16141 gcggttgaga acatcccgca atgtatgaaa gatgcgacca ttgccgtgga agatgggaat
    16201 ttctacagca accccggggt ggatctgacg gggattgtcc gcgcgctgtg gatcaatctc
    16261 agcggtgggg aaacgcttgc gggtgggagt accatcaccc agcaaattgc ccgtaccctg
    16321 ctcttaaagg acgaggtcac agaacgcaca gtgcgtcgaa agttgcgcga gaccgtcctg
    16381 gcctggcaat tgacccaaac ctattccaaa gatgagattc tcgccctgta tctgaatcaa
    16441 atttattatg gcggcatggc ctatggcatc gaagccgctg cacagactta tttcgggaaa
    16501 cccgccgttg acttgttgtt gccagagtgt gcgctgctgg cggggttgcc gcaaaccccc
    16561 ggtatttata acccgttcac caatccagac cttgctttgg atcgccaaaa agtggtgctg
    16621 ggtttgatgg agaaggaggg atttatcaca gccacgcagc ggattgatgc tgaaaattct
    16681 ccattgagct acaatgccga accttacccc atcgaagctc cacattttat ctggatgatc
    16741 aaagaccagt tggacgaact ttatttgtct ggccagttga gtgtggaaca gagtcttatt
    16801 atccgcacca cgttggatct ggatatgcag aaccttgccg aggagatcgt aaaaaggcgg
    16861 atcgagctgt tcaagcctct cgaaggccag accaatttca acgttaagaa tgccgcgctg
    16921 gttgtccttg atccgcatac aggtgaaatc ctcacactgg ttggcagcgc cgattatttt
    16981 gatgcgtcca tcaatggcgc gctcaatatg gccatcaccc gcaggcaatc tggctctgca
    17041 ttcaaaccca ttatttatgc ggcagcactt gacccaacgc gcatggatgc gattactgct
    17101 gcaactccca ttttggatgt tgccacaact tttacaacaa aggacggcca gccctacaag
    17161 ccagtgaatt acgatggcag ggagcatggt tttgtgccag tgcgtcaggc attggcttcg
    17221 tctttgaacg tacccgcagt gctgacttta caaaatgtag gcatcgaaaa tgcgatctcg
    17281 cttgccaatc agttggggat tcgttcactg agtgacccgc gcaattacga cctttctctg
    17341 gcgctcggcg gcgggtctat gaatttgctt gaactttcgg gtgcgtatgc ggcgctggca
    17401 aatgagggca atgtcacagg tttgttttcc gttcttgaaa ttcgcgatgc agatggaaac
    17461 cttctgtacg agcctgaacg ccccgccgca catcgggtcg ttgatgcacg agtggcatgg
    17521 ttgatttccg atatcctcag cgacgaccaa tctcgtgcgc tgggctttgg catcaacagc
    17581 gtcttgaaga ttgaccgcac tgccgcggta aaaacaggca cgaccaccaa cttccacgat
    17641 aattggacca tcggatacac tcccgatttg ctggttggtg tgtgggtcgg aaacagcaat
    17701 caggaagcga tgcacaatgt cacgggtctt acgggcgcgg ctcccatctg gcatgaaacc
    17761 atgcgctcca ttttgcaggg ccgccccgat aaaatgtttt cacagcctgc tggtctcact
    17821 caaatcaatg tgtgcagcct ctcgggtctg ctgcccaccg aggcctgtca gtacacaaaa
    17881 aacgaatggt tcattgaagg cacacaaccc actgcctacg atgcttttta tcaacgtttg
    17941 gatacggggc aggtcgtttt atccctgcct gtcgaagctc aagcctgggc acgttcacaa
    18001 ggactgcctt tgttggattt ggccaatgag ccgaatttcg aagccgattt tctgaccctc
    18061 acatctccaa ccgatcatac gacataccgc atcaccccta acctcgacct gagtgcccaa
    18121 caactggcct tttctgcgcc gacggttccc ggtctcactc aaatgaactt ttttgtagat
    18181 ggtgcttctt tcgcaacgtt gaattccccg ccctttcaag tctggtggcc cttgtctttg
    18241 gggcagcatc ggttttgggt cgaagggatc atgtctacgg gtgagcttgt gaaaagcacc
    18301 atcgtgacca tcaccgtggt ggaataatct ttttggactc ttgtcttcaa ggcggtataa
    18361 ttcgaaaact tatgactaaa ataatgatcg tcgatgacga ccccgaagcc acaagtattc
    18421 tttccagcct gctgaaaatc gaaggatata tcccgatctc agtgaatgac agcacgcttg
    18481 ccattcaaaa ggccaatgcg gttcagcccg atctgttcct gctggatttg atgatgcctg
    18541 tgcttgacgg cttcaaacta tgcagattgc tgcgcgaaga gcggattttc ctgcgtacgc
    18601 cgatcatcat tgtcactgct ttaagcagcg ctgatagcag ggtggtggct tttggcgcag
    18661 gcgccaacga ctacatcacc aaaccttatc tcccacatga acttgtactc aagattcgtg
    18721 aacaattgga acgtgccagg gcaggactga tcgacccatt tgatgggttg taggtgtacc
    18781 aacggatcat cagagggatt tatttcaaag atagcactac gacaattggg aaatgatctg
    18841 agccgccagc atccttcaca atgtaggcat ctgtggcggt aaattctgaa ctgtgaaaaa
    18901 tatggtcaat tcgccagatc ggccccattt gaagccctga atccatgaat gtatatggcg
    18961 gggcagggta actaaaccca actccccagc ctgtttccca atagccatcg atcaagtatt
    19021 gattgatttt tcggtacgct tccgattgat ctgtggcgtt taagtctcct gctacaatga
    19081 gcggaccttt ttcccgcgca atatctgcga caagggcgga catctgcgca tcgtattctt
    19141 gcggtggaat aagaaatggt gggatcgggt gaatgttcca gactgcggcg ggcccctgcg
    19201 gtgctttgat gatgagtttc tgagtccgtc cctggtttgc ttcagcgctg gtccgtttca
    19261 ttgggtatcg gctgtaggca gcctgcccga aatctttgat caccatatct ttgtatggat
    19321 atagatcgtc cagcccatga tatagcggtg agcacaatgc aggggagcat tcctgaacaa
    19381 gcaagacgtc ggggttttca cggcgaatga cttccacaat cccgtcaatt tctggaatgc
    19441 catgcaggtt gtagctcatg atctttactg aaaatccacc ttctgcagaa gaagctgggc
    19501 ggtttggcag gtagagcgga agataagtga agacaatgat aagaatggag cttgtcactg
    19561 tcagccccag ccatttgcgg cgcgtcgccc atgcaaaaaa tagcagggga ataaatagaa
    19621 ataggtacca tgcaaagatg tacgccgtca ttctgacgct caaaagcaag tctcctgtca
    19681 gccatctggc tggatacatt atccacaggc caatgctggg gagccagacc accccctcaa
    19741 atacattacg agcagcggag aatatagttt tcaatttgag acctgatttt gtgaaattgt
    19801 ttgacataac tggccctctg ttttttgttt attgtacccc tcttgctttt gccagaaaac
    19861 cactgtatcc ttgcgccatg gcacctacaa attcaaaaac tgtctggtct tccgaagagg
    19921 gtgaccttcg taaaaaagaa gcggcttctg ccagctccca ttccctgccg ccccaacagc
    19981 agaccattta tctgcaccga gaatcaaaag ggcgcggtgg aaagggtgtg accctggtca
    20041 aaggtctggt actttccgag gctgacctga cggccttggc gaagaaactc aaacaggcct
    20101 gcggcagcgg cggcaccatc aaagatgggg tgattgaaat tcagggcgag caacgtgaaa
    20161 agatcgccga agtgttgcgt tccttgggct ataaagtaaa gatcgctgga gggtgatttt
    20221 tacatcacca gagtgtggac ttttagtccg cttaatgcct gggtgggtca attggcagct
    20281 gtagatggaa cgtacatccg gggaaattga cttcatcgtg gcctttgctc tccacccata
    20341 ctttgccgcc atgtgccctt gccacgccgc ggacaatggc caaccctgcg cctggtccgc
    20401 cgcctttgaa agatgtcttc cctgaagagt gaatggatac attcccgacc tgataaaact
    20461 tctcgaagac aagctcatga tgttcggcat ccagaccgat gccagtatct tgtatgctga
    20521 gttcaacccc gggggcgtgg ttttccatca gcacaggata tgaccgcaga gtgattttcc
    20581 cgccgtcggg tgtgaatttg atcgcattga cgatgagatg gtacagagcc ttttggatca
    20641 aggatgggtc ggcattgatg gcgggtgtat cttcatcctg ttcgacgatg atctcgatgt
    20701 tgcggtcttc ggcagctttg cgaaaatcgc ttgaaagttc gtgcagaatc cgcttcaacg
    20761 gcacgggggc ggggctgatg cgcagttgtt cattttcgat gcgggtgaca tccagcatgg
    20821 tgttgaccac ttcgtgcatc cgttcggagc ctttgacgat tccctccatg acctctttca
    20881 atgcggcgtt ttcctgcagt tccgggaagg agcccagcac attgacatac cctttgataa
    20941 tggtcagcgg agtgcgtagt tcatgtgcgg caacctgaat gaattccatt ttgttgcggt
    21001 ccaactggcg caggaccttg ttgttctttt gaaggtcgag aatggccatt tggtcgtttt
    21061 caagaattcg gtccagtgtg gtgcggatga tgttgatcgc catgctgggg ctgcggctga
    21121 gcatggtctc aaaatctttt ttccccattt cgagcacggt caattcagtg gtggcgcgaa
    21181 cggtggcaga gcgcggcgaa ttttggatca acgccatttc acccaccaaa tcgcctttgc
    21241 cggcaatgcg caagacgcgt tcaccttctt cttcgctgat ctgtttggtg atgaccgcat
    21301 tgccatcggc aatgatatag aagatctctt cgtaggcacc ctcgcggcaa agaatatggt
    21361 cggcaggata ggtgcaaagc cgcgtcagcg ttgccatttc ccgcaggtca ttttctgcga
    21421 ggcctttgaa tgcaaggctc agcagggatg ctttgtcgaa gttaggggtt tcggccattg
    21481 gaacaagtat aaccctggca agtaaccctg actattgcta ttcgattgaa atcttgcatg
    21541 tggattctac ttaatttttt gcaggtggct gtcttttaat ccttttgcgg cacggacgag
    21601 tttaatcaaa actgccgaag ccagcctggc ttcggcagtt ttgaaatttc cacggttcac
    21661 ttattcgctt ggtacgatga agaccatggc aagatatacg aggataccgg gtacgccgcc
    21721 agggatcagg gcgatgagga acgcgaggcg gaaccagaag gtgctcaccc cgaaaaattc
    21781 agccaatccg ccgcataccc cggcgaccac gcgattactg cgggaacgtc gtaatgcttg
    21841 tctttgagta gtcatttgaa cctccaaatt catttgatgt tacagggata tacgcgcgtt
    21901 tt
//
LOCUS       DAOQJC010000062        21656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1090_length_21656_cov_11.085552, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000062 DAOQJC010000000
VERSION     DAOQJC010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 21656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21656
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1090_length_21656_cov_11.085552"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1154)
                     /locus_tag="PLE39_10840"
     CDS             complement(165..1154)
                     /locus_tag="PLE39_10840"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HNE68870.1"
                     /translation="MNSQNTNEVLLKVDDLKMHFPIYRGVFQRQVGAVHAVDGVSFDV
                     MRGETLGLVGESGCGKSTTGRTVLQLYKPTAGNVVFDGVNLVGLKNEEMRKTRRKIQM
                     IFQDPYASLNPRMTVADIISEPLMVHNVGTGKEIQERVAHLLELVNLNPAFATRYPHE
                     FSGGQRQRIGIARALALQPSFIVCDEPISALDVSIQAQVVNLLEELQEQFNLTYLFIA
                     HDLSMVRHISKRIAVMYLGVIMELAERDELYLKPLHPYTQALLSAVPIPDPVADSKRK
                     RIILEGDVPSPVNPPSGCRFRTRCPIADKVCAESRPEFREVTPGHFVACHLVK"
     gene            complement(1154..2143)
                     /locus_tag="PLE39_10845"
     CDS             complement(1154..2143)
                     /locus_tag="PLE39_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68871.1"
                     /translation="MPDLLLDVRGLETQFKTPDGIVHAVNGVSFGLKEGETLGVVGES
                     GCGKSVTMHSVLGLIPSPPGKVTAGEAFFFGQDLLKMTHEEIRHVRGAQISMVFQDPM
                     TSLNPVLTIGRQLQDPLMLHTGMTKNQALERAAELLSLVGIPNAKDRLNDYPHQYSGG
                     MRQRVMIAMALSCSPQILIADEPTTALDVTIQAQIVDLVKRLREELGMSIVWITHDLG
                     VVAGIAQRVLVMYGGFIIEEASVAELFANPSHPYTLGLLGSLPRVDDTEHGRLFSIEG
                     HPPVLYKKPTACPFAPRCKWAMERCWKENPALEQIANEHRVACWVDTKTGRNR"
     gene            2493..2891
                     /gene="mscL"
                     /locus_tag="PLE39_10850"
     CDS             2493..2891
                     /gene="mscL"
                     /locus_tag="PLE39_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNE68872.1"
                     /translation="MLKEFKEFVMRGNVLDLAVAVIIGGAFGKIVGSLVNDILMPLIG
                     LVMGGINFSEKSLTVGAAVVKWGVFIQSVIDFLVVAFVIFLVVKAANSMKKTPPPAPA
                     AAPTTKECPHCFSTISIKATRCPNCTSELK"
     gene            2973..5168
                     /locus_tag="PLE39_10855"
     CDS             2973..5168
                     /locus_tag="PLE39_10855"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNE68873.1"
                     /translation="MEFLKKLNAQQKSAVTAADGPVLVVAGPGSGKTRVLTQRIAYLI
                     ASEGIRPWQILAVTFTNKAAKEMDQRVKAMLNEQATEGMMLGTFHSICARILRREADH
                     LPLESNFVIYDSDDQERIVKAIIRELNLNEKLYRPASVHASISRAKNELIGAEDYPIN
                     TYRDEVVKRVYVEYQKRLISMNAVDFDDLLVYTARLLEDVPAVRDRYAQRFRHVLVDE
                     FQDTNLAQYALVKHLASHHKNIFCVGDPDQSVYAWRGADYRNIKRFEQDFPDAQVVLL
                     EQNYRSHQNILDAATSVIDRARNRHKKRLFSDRGPGEKIFFYEARDDYGEASFVVDTI
                     AQLVASRQFEPGDCAVMYRTNAMSRLLEEAFLQARLPYKLVGAQRFYGRREVKDVIAF
                     IRLIHNLADEASLDRIINVPPRGIGEKTLTTLHLTARQNNTSPGAILLDLARGSDSPF
                     YKSFTGRAALPLADFGGMLATWRAMAATATPAELFEKIVKDINYKEYIVEDDSDESKD
                     RWENVQELKRLALEYSTRTLDEFLENIALVADQDTITDMNAPTLLTLHAAKGLEFGAV
                     FIVGLDDGIIPHSRSFDEPEQMEEERRLFYVGITRAKDKLYLLRAIQRGGRGMSEETY
                     PSRFLEDLPAELLVGKTRTGKPTTSRGLYGRPDTSASDTRWALPTPPKPAPVQTSTYK
                     AGTRVRHGLWGEGIVLDSKLQDGDEIVDVVFESVGIKRLAASLAKLTII"
     gene            5399..5851
                     /locus_tag="PLE39_10860"
     CDS             5399..5851
                     /locus_tag="PLE39_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68874.1"
                     /translation="MQEQKRILLFAFVIMPSHIHAIIKPESDSIGNILQQFGSFTAHE
                     IIKMLKAENKPELLNVFHQKRRDERHEHSIWQDIQAKNVYSTEFLWQKIEYIHQNPIA
                     KGWTLVEDRADYAFSSACHYDNGRKPLIPVTDINEWLSPPPPRTAVGA"
     gene            5883..6866
                     /locus_tag="PLE39_10865"
     CDS             5883..6866
                     /locus_tag="PLE39_10865"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE68875.1"
                     /translation="MQYTNLGKTGLKVSRLCLGMMTYGSKQWREWMLDEEQAKPFVKR
                     ALDAGINFFDTADVYSLGESEKITGNVLRHFGVKRENVIVATKVFSTMSEDVNDRGLS
                     RKHIMDSIDKSLKRLQMEYVDLYQIHRWDYNTPIEETMEALNDVVRAGKARYIGASSM
                     FAWQFMKALHVSEMNGWSKFVSMQNHYNLVYREEEREMIPLCKDQGIGLIPWSPMARG
                     FFAGNRKRGGGGETTRAQSDPFANELYFREEDFVIAERAAGVAKSHNASASQVALAWV
                     LNKPHITAPIIGSSKIEHLDQAIAALEIKLTADEIQLLEELYQPHPILGHA"
     gene            6868..7320
                     /locus_tag="PLE39_10870"
     CDS             6868..7320
                     /locus_tag="PLE39_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE68876.1"
                     /translation="MYIRFLFLGYRIFCFLFRPVRMGVRVMMIQDSKVTLVRQTYLPG
                     WFMPGGGLKRGETLDQAARREAYEETGAELGEIRLMGAYTSFKDWKTDHNLVFVCEDF
                     KITGQPDSEIAEIQSFPLNALPEGLWPGHVRRLEEYKAGKPNPQFGEW"
     gene            7322..7885
                     /locus_tag="PLE39_10875"
     CDS             7322..7885
                     /locus_tag="PLE39_10875"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNE68877.1"
                     /translation="MELKLLWEIFVLFSRVALFSWGGGPASLALMQREVVTAGWATTE
                     EFADAVAVGNALPGPIAPQVSAYVGYKMSGLWGAVSATMGTVFPTTLLMLVMVIYFFS
                     IKDSPAVAAMLKAVRPIVVGLLLWTAYTMAYSVFSVSKVGWAKGLVQNWDKVGIAIAA
                     FTLLTLTEINPVWLVIAGAVLGFIVYK"
     gene            7899..9617
                     /locus_tag="PLE39_10880"
     CDS             7899..9617
                     /locus_tag="PLE39_10880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNE68878.1"
                     /translation="MLKNLPSTPEAILAMEWPDYEPHFKALQSVELTAANIGEWLSDW
                     SALADRLDEQYTRRFVATTQYTTDEEIEKNFNHFIESIQPNARTEEQKLKEKLLASGL
                     QPENFKMGLKKMRAEADIFRQANLELLTEEQKLNGEYDKILGAQTMMWEGEERTIAQM
                     FPLYQDKDRAVREKAWKLVAEVRVQNREAIHSLWKKYMDLRLKITKNAGMSNYRDYMW
                     AQRFRFDYSPADCKTFHDAIEEVVVPAATRLYEKRRKKLGLETLRPWDQMVDTSNRGQ
                     LKPYQTIEEFKSRSKDVFTKVDPKFGEYFQVMMDNDLLDLESRKNKAPGAYNLSYGVS
                     QLPFIFMSNTGTPMDVDTILHEGGHAFHAFEAAVVPYFHERAENYVPAEFAEVASMAM
                     ELLASPYITREHGGFYTKGEAARARFEHLEGIVTFWPYMALVDAFQHWIYENPQISSD
                     PVQCDKKWGELWDRFLPGVDYSGLEDAKTTYWHRQGHIHTTPFYYIEYGLAQLGAVQV
                     WANALKDQKKAVENYRKALALGSTVSLPELFETAGAKFSLDAKTFKVYVDLIEEQMEK
                     LEQMEE"
     gene            9865..10908
                     /locus_tag="PLE39_10885"
     CDS             9865..10908
                     /locus_tag="PLE39_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNE68879.1"
                     /translation="MAETLKIVIPMAGWGTRMRPHTWSKPKPLVSVAGKTSLEHLMDM
                     FKTLPEPENVEYIFIVGPYLGELQVPAFIKEKYPNLKAHYVVQHEMKGQSHALALSRE
                     YLRGPMMVCFSDTLMETDFSFLANEAADGVAWVMPVPDPRRFGVAELDNDGWVTRFIE
                     KPQSMDNNLVVVGCYYFKSAEKLLAAIDEQMQRGIMLKGEYFLTDTITVMIEHGAKVR
                     TNQISTWLDTGTIEATLDTNKTLLESRSAGAFERSNVETLKRSNVNIVEPSFIDPSAE
                     ISNSTIGPYASIGANCKIENCQIAESIIEAGCEIKDAALSRSLIGRQARVKARGDGHV
                     MQLNIGDTSSVEL"
     gene            10911..11120
                     /locus_tag="PLE39_10890"
     CDS             10911..11120
                     /locus_tag="PLE39_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68880.1"
                     /translation="MEAVECRSDTEYAERPLSLLWRGVRYEIAEIISRWRGPGEKGFC
                     VKTTDGFTFELTYQEISDDWHVQPI"
     gene            11130..12317
                     /locus_tag="PLE39_10895"
     CDS             11130..12317
                     /locus_tag="PLE39_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE68881.1"
                     /translation="MQAASEISNVNRLSKRVAELKPSGIRKFFDIVATMKDVISLGIG
                     EPDFTTPKPILDAGIRSLQNGETHYTSNHGKVELRQGVAENLQKLYNVKYDPLSEVVA
                     TVGVSEALYLTFTAILEPGDEVIIPTPCFVSYQAEVILAGGVPIEIPARLENNFTIDP
                     DDIRKAITPRTKVIFIGYPSNPTGAVAPREVMVEIGRIAEQYNLIVVSDEIYDRLVYD
                     FEHVCFPALDESLRRRTVLLGGFSKAYAMTGWRIGYACGPADIIQGMVRVHQYTIMSA
                     PTTAQDAAIEALKNGEPHVQEMVTEYNRRRRLLVDGLNRLGLSTFEPRGAFYTFPNIQ
                     ATGMDDETFAEALLREEGVAVVPGNAFGPGGEGFVRACYATEYSQIEEALRRMERFMS
                     RHG"
     gene            12438..13295
                     /locus_tag="PLE39_10900"
     CDS             12438..13295
                     /locus_tag="PLE39_10900"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNE68882.1"
                     /translation="MANVKITSSLVSVQWLAENLEAENLIILDASMKPVTPVGNASSD
                     QAGCIKGALRFDFDNDIREKNTDLPHMMPTAEFFTEEMQKLGISQDSAIVVYDYVGVY
                     SSPRAWWMFRAMGHEQVAVLNGGLPAWKQAGLPCGEQAETKASRRGDFVAHPQAGLFC
                     DSTHVREALADSAFAVLDARSPGRFKGVEPEPRAGLRGGRMPNSLNLPFADVVVNGSM
                     QPPAQLESIFSKLVKKEQKLIFSCGSGVTACIDALAAELAGYQNISVYDGSWAEWGSP
                     SSGLPVEQG"
     gene            13355..13726
                     /locus_tag="PLE39_10905"
     CDS             13355..13726
                     /locus_tag="PLE39_10905"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE68883.1"
                     /translation="METTLTVTQEKIQAHIPITVFHLQGWLDARGEETLLAAARDAYS
                     EGARHLVLDLAGVETLTSAGMRAMHKVYKLFTPTEERMKRLHVAISNAPSQVNNILGI
                     TGFLLNIPNYRNIQSAVESFK"
     gene            13846..15069
                     /locus_tag="PLE39_10910"
     CDS             13846..15069
                     /locus_tag="PLE39_10910"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE68884.1"
                     /translation="MKSTFRALRDKKFAALLLGQTFSRIGDFLFQIALAWWVLEKTGS
                     ATAMGTVLFFSTLPMVAFVLVGGVVVDRMRRGLLLFISDTARCFIMVIGAWLAFTDQL
                     VLWEIYVIAILFGFADAFFLPAYNALIQQIVAEEDLPSANSINSLSMQFGRVAGPALG
                     GLIAGFGGTAVAFGFNALSFFIGALTVIPLLNLPVPASSEESGFTLQHFKDDLRDGFN
                     TIFSTPVLWVSIIVFAFANITLAGPYSIAMPFLVQEKLGGDEKMLGFIYSIFPIGYAI
                     ASLVMGGFRKIRYRGILLYVCGAIAGIGLGVFGFGLPLYLLVIAALINGAALEIDGLI
                     WTNLMQEKVPPEKMGRVSSVDSLGSFVLLPIGYAFTGWLIDTIGVSSVFIAAGVVTAL
                     VSLLPLLHPTIRKLD"
     gene            15211..16164
                     /gene="msrP"
                     /locus_tag="PLE39_10915"
     CDS             15211..16164
                     /gene="msrP"
                     /locus_tag="PLE39_10915"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNE68885.1"
                     /translation="MTDLFKTVPIRSSEITPKSVYLSRRDFMKAATFTTGAALLTACA
                     PSVAGAAEDNAPVPTSNATDELGDPVNSYTDITNYNNYYEFTVDKQQVADEARGFNLT
                     PWTVEVGGLVNNPKTFGIEDLLKLFPQEERIYRLRCVEAWSMVIPWTGFTLASLLEMV
                     EPTSDAKYVRFETVYRPEEMPGQKSPFYPWPYQEGLRLDEAMNDLTLLATGLYGEPNV
                     AQNGAPIRLVVPWKYGFKSIKSIVKIELVAEEPSTLWSNIASNEYGFYANVNPDVDHP
                     RWSQASERRIGELNRRPTLPFNGYGEQVAHLYDGMDLSVNY"
     gene            16230..16907
                     /locus_tag="PLE39_10920"
     CDS             16230..16907
                     /locus_tag="PLE39_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HNE68886.1"
                     /translation="MKKFPYTPLQIAVHLYAWSGLALLLLDYFTGNLSPNPIQDLEQR
                     TGRHAITLLVLSLLCTPINTIFKWSEPIKRRRALGVYAFLYATVHMIIFVDLDFGLAW
                     SSVIKEVIEKPRLIVGVIAFILLIPLALTSFDIWKKRLGKNWKRLHQLVYLIAPLVFL
                     HYVWSKKGDLLLLQGEILKPLIYGLIIGIFLVLRIPPIRKAVASFSTRSLALLNKKNP
                     QPEVDTP"
     gene            complement(16952..17179)
                     /locus_tag="PLE39_10925"
     CDS             complement(16952..17179)
                     /locus_tag="PLE39_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68887.1"
                     /translation="MTTQTKQHIPWYLWPFAALWKLLAVIVELTGRFVAMVLGLVLIL
                     VGGIVSLTVIGAIIGIPLAIIGLLLLLRGIF"
     gene            17266..18624
                     /locus_tag="PLE39_10930"
     CDS             17266..18624
                     /locus_tag="PLE39_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNE68888.1"
                     /translation="MKSKFNKYWLIPIVLLLAVGIYYLPPVHSRLAWRVDALRVQVIY
                     FFNPPDEVVFEPSGDTSPLTIETAIATARAEYMLTLTPQYTPTVTPTTGPTARPTATS
                     TPLPEAVNLEGVVYVDQHERWNYCGPANLTMALNFWGWKGNRDDVAKIVKPGSGDTKV
                     NFIDRSKDDKNVMPYELVDFVNENTEYRALMRHGGDENLVKRLLAAGFPVVVEKGYYE
                     HDTTGKISWLGHYLFTTGYDDARGGFIVQDAYTKPGKNLLSDYATYLEGWRAFNYQFI
                     VVYPAEREAEVLTLLGPWVDENWAAQHALELAEKDVQSINDNNLFFAWFNKGTSHVLL
                     KQYVDAAAAYDQAFSTYANWDEKEQERPYRIMWYQTGPYFAYFYSARYADVISLADTT
                     LFDPANKKGRTLEESLLWRGRAYYMSGNTQAAIDDYRAALRIHVNWPPAVQALQDLGL
                     QP"
     gene            18716..19945
                     /locus_tag="PLE39_10935"
     CDS             18716..19945
                     /locus_tag="PLE39_10935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HNE68889.1"
                     /translation="MRVLHFADAHIDMANYGRHDPQTGLPMRVLDFLKSLDTIVDTAI
                     SQKVDLVIFAGDAYKDRSPAPTFQREWGRRIMRLSQAQIPTLLLIGNHDISPAVGRAH
                     ALQEFKTLQVPFVKVLDQPSFLKPEDLWELPMQVIAMPWVTRSGLMANLEMSGTETEQ
                     IYSNIEERIASLVDEWVSEADKNLPIIFTAHASIEGAKFGGERLVMLGNDLVLSGSLV
                     KNQRFNYVAMGHIHKPQDVNEGHQPPVIYPGSIERVDFGEAKEDRFFVIAEIESGKET
                     QVEWVQLTGARKFIDRRTVLRSNENVTEALKDALPSLKEMSEAIVRLSVEYPREWDTL
                     IDETALRKYTEDAFEFHLVKRPQSEARVRIPEGQVVSSLSPLDLLAQYFDAAKVKDAD
                     ELNKLAQEIISEDTLEN"
     gene            20012..21295
                     /locus_tag="PLE39_10940"
     CDS             20012..21295
                     /locus_tag="PLE39_10940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE68890.1"
                     /translation="MSKENSRYRWFVVVVFFFFMLLHQTDKLMIGSMQVDISKDFNLN
                     NQQWGLINSGALIVATILYPIWGYLYDRYSRTKLLSLASFIWGATTWLNAIVKTFGGF
                     LTTRASTGIDDSSYPGLYTLIADYFGPNLRGKIYGILQLAQPIGYLVGMILALMVAPA
                     IGGWRSVFYITGSLGLVIAVLIYFGVREMPRGQAEPEFENMPEMQTFKFSWAEAREIF
                     KKKTMWFVFLQGFAGVFPWNVITFFFFAYLEKERGYDANGILFTMAPVILILASGYFI
                     GGALGDMAFKRSNKGRIVVSSIGVLLGALFMYLAITTPVDAKNQFFILMCLTAVFMPL
                     SSANVIATVYDVTVPEVRSTAQAVEYFIENAGAAFAPILTGYIADLYDLKTAILIICT
                     AAWLLCFVFYLGAIFTIDKDSHDLREQMAARAKSM"
BASE COUNT         5446 a         5681 c         5497 g         5032 t
ORIGIN      
        1 ggccatgctg gcgatgacga gaaggctcag taaagcaaac attttacgca tttttcttct
       61 cctcgcaaaa tttggaaatt ttggtttaaa aaacttacat tgtttgacag tgtggacatt
      121 tacggtcact tacttgcagc aatcacctcc tttaccagga aagattattt gaccaaatga
      181 caggccacaa aatggcctgg agtaacttca cgaaattcag gtcgggattc agcacaaacc
      241 ttgtctgcaa ttgggcagcg tgtgcggaaa cgacaacccg aaggtggatt caccggtgac
      301 gggacatctc cctcaaggat gatacgcttg cgcttggaat ctgcaaccgg gtctggaatg
      361 ggcactgccg aaagcaaggc ctgtgtgtat ggatgaaggg gcttgagata cagttcgtcg
      421 cgctccgcca attccatgat cacacccaga tacattactg caatgcgctt gctgatatgg
      481 cgaaccattg aaaggtcatg cgcaatgaac aggtatgtca ggttgaattg ttcctgcaat
      541 tcttccagca ggttaaccac ctgcgcctgg atcgaaacat ccaacgcaga gatgggctca
      601 tcgcagacga taaaggaagg ttgaagcgcc aaagcgcggg cgatgccaat acgctgacgc
      661 tgtccgccgg agaattcatg cgggtaacgg gtggcgaatg ccggattcag gttgaccagt
      721 tcgaggagat gggcgacgcg ttcctgaatt tccttgccag taccgacatt atgaaccatc
      781 aaaggttcgg agatgatatc ggcaactgtc atacgggggt ttaagctggc atagggatct
      841 tgaaagatca tctggatctt gcggcgggtc ttgcgcatct cttcgttttt caggccaacc
      901 aaattaacgc catcaaacac cacattgcca gcggtcggct tgtacaactg taaaaccgtt
      961 ctacccgtag tcgactttcc gcagcccgat tcacccacca agcctaatgt ttcgccacgc
     1021 atcacatcaa aggaaacacc atccacagca tgaacagcac caacttggcg ctggaacaca
     1081 ccacgataaa tggggaaatg catcttaagg tcatcgacct tcaacaatac ttcatttgta
     1141 ttctgcgagt tcattagcgg tttctccccg ttttggtgtc gacccagcag gccacacggt
     1201 gttcatttgc gatctgctca agggccgggt tttccttcca acatctttcc atggcccatt
     1261 tacaacgcgg ggcgaacgga catgcagtcg gttttttgta gaggactggg ggatgtcctt
     1321 cgatggagaa caaacgacca tgttcggtat catctacacg aggcaggctt cccaagagac
     1381 ccagggtgta aggatgggat ggattcgcaa acaactcagc gacggaggct tcttcaataa
     1441 taaagccgcc atacatcacc aaaacacgtt gagcgatacc agccacaacg ccgagatcat
     1501 gggtgatcca gacaatggac atgcccaact cttcacgcag acgtttgacc aaatccacga
     1561 tttgagcttg aatggttaca tccaacgccg tggttggttc gtctgcgatc aaaatttgcg
     1621 ggctgcacga aagcgccatg gcgatcatca cgcgctggcg cataccgcct gagtattgat
     1681 gtgggtaatc gttcagccgg tctttggcat tggggatgcc aaccagactc aacaattcgg
     1741 cagcacgttc caatgcctga ttcttggtca taccggtgtg caacatcaac gggtcctgca
     1801 attgacggcc aatcgtcaac acagggttca gggaggtcat gggatcctga aagaccatgc
     1861 tgatctgcgc accgcgcaca tggcgaattt cttcatgtgt cattttaagg agatcttggc
     1921 caaaaaagaa cgcttccccc gccgtgactt ttcctggagg tgagggaatc aaacccagta
     1981 ctgagtgcat agtaacgctc ttaccgcaac cactttcccc cactacaccg agtgtctcac
     2041 cttctttgag tccgaaagag acaccattta ctgcgtgaac aatcccatct ggggtcttga
     2101 attgagtctc aagccctctt acatctaata aaagatcagg catccgctag gtccttttct
     2161 aaattgggat aataactgcc tgcatttttc tggctgcagg cgaaccgaat tatacctaat
     2221 tccattaagc cgtcaacacg acaaacttta tgactggctt gtccagtcca gtacatgtat
     2281 aaaaatacac caaaagactg gaatccaccc cctactggag aactgttttt ttaacccttg
     2341 tttaaagccg cttgtcatac aaatcagaga tcagaagttc caaaacacag gcagggattt
     2401 gtaccggaga catttcatgg gtgcagtttg ttgacaagcc ccccttccta aaatagaatt
     2461 caaagccaag ccatcatcac aaggagaatc ccatgttaaa agaattcaaa gagttcgtca
     2521 tgcgcggcaa tgtgctcgac cttgccgtag cggtcatcat cggcggtgcc tttggcaaga
     2581 tcgtcggttc gctggtcaat gatattttga tgccattgat cggattggtc atgggcggga
     2641 tcaacttcag cgagaaatcc ctgaccgttg gcgctgccgt ggtcaaatgg ggtgtgttca
     2701 ttcaatcggt catcgacttc cttgtcgttg cctttgtcat cttcctggtt gtcaaagcag
     2761 ccaatagcat gaagaagacc ccacccccgg cacccgctgc agccccaacc accaaagaat
     2821 gccctcactg cttctctacc atttccatca aggctacccg ctgccccaac tgcacatctg
     2881 aattgaaata agcatttccc ttcaacattc aaaatgatat tcaccgctct ggcgggggca
     2941 atgcccccgc cagagcattt ataatggcgg acatggaatt tctcaaaaaa cttaatgcac
     3001 aacaaaagag cgctgtcaca gcggcggatg ggcctgtttt ggtcgtggca ggcccggggt
     3061 cgggaaagac gcgggtgttg actcaacgca tcgcttattt gatcgcatcg gagggaatcc
     3121 gcccatggca gattcttgcg gtcacgttca ccaataaagc tgcgaaggaa atggatcaac
     3181 gtgtaaaggc catgctgaac gagcaagcca ccgaggggat gatgctcggc acgttccatt
     3241 cgatttgcgc gcgcattctc aggcgtgaag cggaccatct gccgcttgag tcaaattttg
     3301 tgatctacga ttcagacgat caggaacgca tcgtaaaagc gatcatccgt gagttgaatc
     3361 tcaacgaaaa gttgtatcgt cccgccagcg ttcacgcatc catttcacgc gcgaagaatg
     3421 aattgatcgg tgcagaggat tatccgatca acacctaccg tgacgaagtg gtgaaacgtg
     3481 tgtatgttga atatcaaaaa cggctgatct caatgaatgc cgtggacttt gatgacttgt
     3541 tggtgtacac cgcacgattg ctggaggatg tccctgccgt gcgtgatagg tatgcccagc
     3601 gcttccgcca tgtgttggtg gatgaatttc aagataccaa ccttgcgcag tatgccttgg
     3661 tgaaacacct tgcttctcat cacaaaaaca tcttttgcgt tggcgacccc gatcagagtg
     3721 tgtatgcctg gcgcggtgcc gactatcgca acatcaaacg ttttgaacag gatttccctg
     3781 atgcacaggt ggtcctgctc gaacaaaact accgctcgca tcaaaacatt ctggatgccg
     3841 ccaccagtgt gatcgaccgt gcgcgcaacc gccacaagaa aagactgttc agcgaccgcg
     3901 gcccgggcga aaagatattt ttctacgagg cccgcgacga ttacggcgaa gcatcttttg
     3961 tggtggatac catcgcccaa cttgtcgcca gcaggcaatt cgaacccggc gactgtgcag
     4021 tgatgtaccg taccaatgcc atgtcgcgtc tgcttgaaga agcgttcctg caggcacgcc
     4081 ttccttacaa gctcgtcggc gcgcaaagat tttatggacg ccgcgaagtt aaagatgtga
     4141 tcgccttcat acgccttatc cacaaccttg ccgatgaagc cagcctcgac cgtattatca
     4201 atgtaccgcc gcgcggcatc ggtgaaaaaa cgctgaccac ccttcactta accgcccgcc
     4261 aaaacaacac ctcacccggt gcgatcctgc ttgatcttgc gcgcggcagt gactcgcctt
     4321 tctataaatc cttcacaggc cgtgccgcac tacccctcgc tgatttcggc gggatgctcg
     4381 ccacctggcg cgccatggcg gccaccgcca cccccgccga actctttgaa aagatcgtta
     4441 aagatatcaa ctacaaagaa tacatcgtcg aagatgattc tgatgaaagc aaagaccgtt
     4501 gggaaaacgt gcaggaactc aaacgcctcg cgcttgaata ttccacacgc acgcttgatg
     4561 aattcctcga aaacatcgcg cttgtcgccg atcaagacac gatcacagac atgaacgcgc
     4621 ccaccctgct caccctgcat gccgcaaaag gacttgagtt cggcgcggtc ttcattgttg
     4681 gcctcgacga tggcatcatt ccacacagcc gctctttcga tgaacctgaa caaatggaag
     4741 aagaacgccg cctcttttac gtcggcatca cccgcgcaaa agataaactc tacctgttgc
     4801 gcgccattca acgcggtgga cgcggcatgt cggaagagac ttatccctca cgttttctcg
     4861 aagacctgcc cgccgaattg ctggttggga aaacacgcac cggcaaaccc accacctcac
     4921 gcggattata cggacgcccc gacacgtctg cctccgatac aagatgggcg cttccgactc
     4981 cgcccaaacc tgctcctgtt caaaccagca cctacaaagc tgggacaagg gtccgtcatg
     5041 gtttgtgggg cgagggcatc gtactcgata gcaaattaca agatggcgac gagatcgtgg
     5101 atgtggtctt tgaatcggta ggtatcaaac gattggcggc aagcctcgca aagctgacaa
     5161 tcatataaac acactggaaa gtggacggta caaaaaacgg tttaccaacc actatccacc
     5221 atccaaatgt gctcacagcg ccgtccccgg cgcggggtgg caggaagcaa tcttcatgac
     5281 cttttcttcc tcacatgacc ctgcccactt atatttcata accgcatccg tcatagaatg
     5341 gaagcatcta tttgcaatgc ctgaatatgc aaaaattcca atggattccc tggcatggat
     5401 gcaggaacaa aaacgaattt tgttgtttgc gtttgtaatc atgccttctc acatacatgc
     5461 aattatcaag ccagaatccg attcaattgg aaacatcctt cagcaatttg gttctttcac
     5521 agcgcatgag atcatcaaaa tgctaaaagc cgaaaacaag ccggaactgt taaacgtgtt
     5581 tcatcaaaaa cgcagggacg aaagacacga acatagcatc tggcaggata ttcaagccaa
     5641 gaacgtttac tcaacagaat ttttatggca gaaaatcgag tatattcacc aaaaccccat
     5701 agccaaaggc tggacacttg tcgaagaccg ggcagattac gctttttcca gcgcctgtca
     5761 ttatgacaat ggtagaaagc ctttgatccc tgttacagac ataaatgaat ggctatcccc
     5821 tccaccgccg aggacggcgg tgggagcata aaatctacca tccaacatca aggagattca
     5881 caatgcaata caccaacctc ggcaaaactg gattgaaagt ctcgcggctg tgtttgggga
     5941 tgatgaccta cggctcgaag caatggcgcg agtggatgtt ggatgaagaa caggccaaac
     6001 cattcgtaaa acgtgcattg gatgcgggaa tcaatttttt cgacacggca gatgtttatt
     6061 cgctgggtga aagcgaaaaa attacgggga atgtacttcg tcactttggt gtaaaacgcg
     6121 aaaacgtgat cgtcgccacg aaggtgttct ccaccatgag cgaggacgtg aacgaccgcg
     6181 gtctctcacg caaacacatc atggactcca tcgacaaatc cctgaaacgc ctgcaaatgg
     6241 aatatgtgga tttgtaccag atccaccgct gggattacaa cacccccatc gaagagacaa
     6301 tggaagcatt gaacgatgtt gtccgtgcgg ggaaggcgcg gtacattggc gcatcttcga
     6361 tgtttgcgtg gcaatttatg aaggcgctgc atgtttccga gatgaatggc tggtcaaaat
     6421 tcgtttcgat gcagaatcac tataaccttg tatatcgcga ggaagagcgt gagatgatcc
     6481 cgttgtgcaa ggatcaggga attgggttga ttccgtggag cccaatggca cgcggatttt
     6541 ttgcaggcaa ccgcaagcgc ggcggaggcg gcgagacgac acgcgcacaa agcgatccat
     6601 ttgcgaacga gttgtatttc cgcgaggagg attttgtcat tgccgaacgt gcggcgggag
     6661 tggcgaagag tcacaatgca tcggcttcac aggtggcatt ggcctgggtg ttgaacaaac
     6721 cccatatcac cgcacccatt atcgggtcga gcaagatcga gcatttggat caggccattg
     6781 ccgcattgga gatcaaactc acagcggatg aaatccaatt gctcgaagag ttgtaccagc
     6841 cgcatccgat tttgggacat gcctaacatg tatatccgtt ttctattcct tggctatcgg
     6901 atcttttgtt ttttgtttcg gcctgtgcgc atgggtgtgc gtgtgatgat gatccaggat
     6961 tcaaaagtga cgcttgtgcg gcagacgtat ctaccgggct ggttcatgcc cggcggcgga
     7021 ctcaaacgcg gcgaaacatt ggatcaagct gcacgccgcg aagcctatga agaaacaggc
     7081 gcggaactcg gggagatccg tttgatgggt gcgtacacca gcttcaagga ttggaagacc
     7141 gatcacaatc ttgtttttgt ctgcgaagat ttcaagatca caggccaacc cgacagtgag
     7201 atcgctgaaa ttcagtcgtt tcctttgaat gcattgcccg aaggattatg gcccgggcat
     7261 gtgcgcagac tggaagaata caaagcgggc aaacccaacc cgcagtttgg agagtggtga
     7321 aatggaattg aagttgctct gggaaatttt cgtgttgttc agccgtgtgg ctttgttctc
     7381 atggggcggc ggaccggctt cactggcctt gatgcagcgc gaggtggtga cggcaggctg
     7441 ggccacgacg gaagaattcg cagatgcagt ggcggtggga aacgccctgc cggggccaat
     7501 tgcgccgcag gtttcggctt atgtgggcta taaaatgtcg gggttgtggg gggcggtctc
     7561 tgcgacaatg ggaacggtat ttccgaccac gttgttgatg ttggtgatgg taatctattt
     7621 cttcagcatc aaagacagcc ctgctgtggc ggcgatgttg aaggccgtgc gacccattgt
     7681 ggtggggttg ttgctgtgga cggcctacac gatggcgtat tctgttttca gtgtaagcaa
     7741 ggtgggttgg gcaaaagggt tggtacaaaa ttgggacaag gtgggtattg cgatcgctgc
     7801 gtttacactg ttgacattga ccgagatcaa cccggtttgg ctggtaatag ccggggcggt
     7861 attgggattt atcgtttaca agtaaaagga gttttctcat gcttaagaat ttaccctcta
     7921 caccggaagc catccttgca atggaatggc ctgattacga accacatttc aaagccttgc
     7981 aatctgtcga attgaccgcc gccaacatag gcgagtggct ttccgattgg tcggccttgg
     8041 cagaccgatt ggatgagcaa tacacgcgcc gttttgtcgc gaccacgcaa tacacgaccg
     8101 atgaagagat cgagaagaat ttcaatcact ttatcgaatc catccagccc aatgcgcgca
     8161 cagaagagca gaagctgaag gaaaagctgc tcgccagcgg tttgcagcct gaaaacttca
     8221 aaatgggtct gaagaaaatg cgcgctgaag cggatatctt caggcaggcc aaccttgaat
     8281 tgctgaccga ggaacaaaaa ctgaacgggg aatacgacaa gatcctcggc gcacagacca
     8341 tgatgtggga aggggaggaa cgcaccatcg cgcaaatgtt cccgctgtat caagacaagg
     8401 atcgcgcggt ccgcgaaaag gcgtggaaac tggtggcgga ggtgcgtgtg cagaaccgcg
     8461 aagcgatcca cagcctgtgg aaaaagtaca tggacttacg gctcaagatc acaaaaaatg
     8521 ccggtatgtc caactatcgt gattatatgt gggcgcaaag attccgcttc gattattcac
     8581 ccgccgattg caagaccttt catgatgcga tcgaagaggt tgttgtccct gcggcgaccc
     8641 gcctctatga aaaacgcagg aagaaacttg ggcttgaaac gctgcgtccg tgggaccaga
     8701 tggtggatac atcgaatcgt ggtcaactca agccgtatca aaccatcgaa gaattcaagt
     8761 ccaggtcgaa ggatgtattt accaaggtgg acccgaagtt tggcgaatac tttcaagtga
     8821 tgatggataa cgacctgctc gaccttgaaa gccgtaagaa caaggcgccc ggggcataca
     8881 acctgagcta tggggtcagc caattgcctt ttatctttat gagcaacacc ggcacgccga
     8941 tggatgtgga caccatactg catgaaggcg gacatgcctt tcatgcattc gaagcggcgg
     9001 tcgttccata tttccatgag cgcgccgaga attatgtccc tgctgaattt gcagaagtgg
     9061 catccatggc gatggaattg ctggcttctc cctacatcac cagggaacat ggaggtttct
     9121 acaccaaggg cgaagctgcc cgcgcccgct tcgaacattt ggaagggatc gttaccttct
     9181 ggccgtacat ggcattggtg gatgccttcc aacactggat ctatgaaaac ccgcagatca
     9241 gttcagaccc ggtgcagtgt gataaaaaat ggggggaatt gtgggatcgc ttcctccccg
     9301 gcgtggatta cagcggactc gaggatgcca aaacgactta ctggcatcgt cagggacaca
     9361 tccacacgac tcctttctat tacattgaat atggtctcgc tcaattgggc gcggtgcagg
     9421 tgtgggccaa tgcactcaaa gaccagaaga aagctgtgga aaattaccgc aaggcgctgg
     9481 cgctcggttc cacggtttca ctgcctgagt tatttgaaac agcgggagca aaattctcac
     9541 tcgatgccaa aacgttcaag gtgtatgtgg atctgatcga agagcagatg gaaaagcttg
     9601 agcaaatgga agaataaata accaggattc tttgtagatc ggtgcgtgaa ttaaagagac
     9661 agtcacttat gaggtgactg tctctttttt gtaaccgcaa acttagagtt gtcatcaacc
     9721 gccgctgaag gaactggctc ttgatagtga ggcgtagtgc gggataatat cccgcactac
     9781 gatagatagg acgggagttt ttttatatcg ctggcaggta ttcattggta aaataccctt
     9841 gaaatcattc acaggagaaa tctcttggct gaaaccctaa agatcgtcat tcccatggct
     9901 ggctggggaa cgcggatgcg cccgcatacg tggagcaagc cgaagccgct ggtaagtgtg
     9961 gcgggcaaaa cgtcacttga acatttgatg gatatgttca agactctgcc ggaacctgaa
    10021 aatgtggaat acattttcat tgtggggcca tatctcggcg aactgcaagt ccctgctttt
    10081 atcaaagaaa aatacccgaa cctcaaagcg cactatgtag tgcagcacga aatgaaggga
    10141 caatcacacg cgttggcgct ttcacgcgaa tatttgcgcg gcccgatgat ggtctgtttt
    10201 tcagacactt taatggagac agatttctcc ttcctcgcga acgaggcagc cgatggcgtg
    10261 gcctgggtga tgccggtgcc tgatccgcgc cggttcgggg tggcggagtt ggacaacgac
    10321 ggctgggtga ctcgcttcat tgagaagccg caaagcatgg acaacaacct tgttgtggtt
    10381 gggtgttatt acttcaaaag cgccgagaaa ctccttgccg ccatcgatga acaaatgcag
    10441 cgcggaatca tgctcaaggg cgaatacttc ctcaccgaca cgatcacagt gatgatcgaa
    10501 cacggagcaa aggtccgcac gaatcagatc agcacctggc tggatacggg aaccatcgaa
    10561 gcgactttgg acacgaacaa gaccttgttg gaaagtcgga gcgccggggc gtttgaacgt
    10621 tcaaacgttg aaacgttaaa acgttcaaac gtgaacattg ttgagccttc attcatcgat
    10681 ccaagcgcgg agatttccaa ttccaccatt gggccgtatg cttcgatcgg cgcaaattgc
    10741 aaaatagaga actgccagat cgcggagagc atcattgaag caggttgtga gatcaaggat
    10801 gcggcgctga gtcgctcgtt gatcggcagg caggccaggg tcaaggcgcg aggcgatggt
    10861 catgtcatgc agttgaacat cggcgataca agttcggttg aactgtagcc atggaagctg
    10921 tggaatgtcg ttccgacact gaatatgcgg aaaggccgct ctctctttta tggcggggag
    10981 tccgttatga aattgcagag atcatttcgc gctggcgcgg ccccggtgaa aaaggttttt
    11041 gcgtgaaaac gaccgacggt tttacatttg aattaacgta ccaggaaatc tcagacgact
    11101 ggcacgttca acccatataa ggaggctcaa tgcaggcagc aagtgaaata agcaatgtga
    11161 atcggttgtc gaaacgtgtg gctgaattaa agcccagcgg cattagaaaa tttttcgata
    11221 tcgttgcgac catgaaggat gtgatctctt tgggaatcgg tgaacctgat ttcaccacac
    11281 ccaaaccgat cctcgatgcg gggattcgtt ccttgcaaaa tggtgaaacg cattacacat
    11341 ccaaccacgg caaggttgaa ttacgtcagg gagttgcgga gaacctgcaa aaactctaca
    11401 acgtaaaata cgatccgctt tccgaggttg tggcgaccgt gggtgtttcg gaggcgttgt
    11461 atctcacctt caccgccatt ctggaaccgg gcgatgaggt catcattccg accccatgtt
    11521 ttgtttccta tcaggcggaa gtgatcctgg ctggcggcgt gccgatcgaa attccagcgc
    11581 ggctcgaaaa caacttcacc attgacccgg acgatatccg caaggccatc accccgcgca
    11641 cgaaggtcat ctttattggt tatccatcca accccaccgg ggccgtggcc ccgcgcgaag
    11701 tgatggtgga gatcggcagg atcgcagaac aatacaacct gatcgtggtc tccgatgaaa
    11761 tttacgaccg ccttgtgtac gacttcgaac acgtctgctt ccctgcattg gacgaaagcc
    11821 tgcgccgccg cacggtgctg ctcggcgggt tctccaaagc ctacgccatg acaggctggc
    11881 gcatcggcta cgcctgcggg cctgccgata tcattcaagg catggtgcgt gtccatcaat
    11941 atacgatcat gtctgcgccg accactgcgc aggatgccgc cattgaagcc ttgaagaacg
    12001 gagagcccca tgtgcaggaa atggtgacgg aatacaaccg ccgccgtcgt ctgctggtgg
    12061 atggattgaa ccgcctcggt ctttcgacct ttgagccgcg tggtgctttc tatacattcc
    12121 ctaacatcca ggccaccggc atggacgatg aaaccttcgc cgaagccctg ctgcgtgaag
    12181 agggtgtggc cgtggtgccg ggcaatgcct tcggcccggg tggagaggga tttgtccgcg
    12241 cttgttacgc caccgaatac agccagatcg aagaagccct gcgccgcatg gaaaggttca
    12301 tgagcaggca cggatagatt tggtgaatag taagtagtga tcagtgggac aggcctcgta
    12361 aaacggcctg tcccattttt atttaaactc ataacgtatc gtataatttt caaaaccgac
    12421 aaaacaggtg gcgcatcatg gcaaacgtaa aaatcacatc ttcattggtc tctgtgcaat
    12481 ggctggctga aaatctcgag gcggaaaatc tcatcatttt ggatgcaagc atgaaaccgg
    12541 tcaccccggt gggaaacgct tcttcagatc aggcgggctg tatcaaaggt gcattgcgat
    12601 tcgattttga caacgacatc cgcgaaaaga acactgacct gccgcacatg atgcccacag
    12661 cagaattctt cacagaggaa atgcaaaaac tgggcatcag ccaggacagc gccatcgtgg
    12721 tctatgacta tgtgggcgtg tactccagtc cacgggcatg gtggatgttc cgcgccatgg
    12781 gacatgagca ggtagccgta ctcaatggcg gcctgccagc atggaaacaa gccgggctcc
    12841 catgcggaga acaggctgaa accaaagcct ctcggcgcgg agatttcgtg gcacatccgc
    12901 aggccgggtt gttctgcgat tctacgcatg tacgtgaggc gcttgccgat tccgcttttg
    12961 ccgtgttgga tgcccgctca ccggggcgct tcaaaggggt ggagcccgag ccgcgtgcag
    13021 gtctccgggg cgggcgcatg cccaactcgc tcaacctgcc atttgccgat gtggtagtga
    13081 atggatccat gcagccgccc gcgcaactcg aatccatttt ttcgaaattg gtaaaaaagg
    13141 aacagaaact catctttagc tgcggatcgg gtgtcacagc ctgcattgat gcgcttgcag
    13201 cagaactggc aggctaccaa aatatttccg tctatgatgg ttcatgggcc gagtggggat
    13261 ccccatcttc aggattgccg gtggaacagg gataatccaa aggtcatggg agaggcgcgc
    13321 actcccttgc atcagatgta taatcttaaa catcatggaa accacattaa cggtcaccca
    13381 ggaaaaaatt caggcgcaca ttcccatcac cgtgttccat ctgcaaggct ggctcgatgc
    13441 aagaggagag gaaaccctgc tcgccgccgc ccgcgatgca tacagtgaag gcgcccgtca
    13501 tctggtgctc gaccttgccg gggtggaaac gctcaccagc gcagggatgc gggccatgca
    13561 caaggtctac aaactgttta cacccaccga agaacgcatg aagcggctgc atgtggccat
    13621 cagcaatgcc ccatctcaag tgaataacat ccttggcatt acggggttcc ttttaaatat
    13681 tcccaactat cgcaacatac aatcagcggt cgaatcattc aagtgaccgt gcccgtccat
    13741 agttttcttt gaaaatcttt cttccctgcc gccaaaatgc caagaagaaa gattgctttt
    13801 attaaataca aggcgagtca tcaattttag aatcagaggt gtgaaatgaa atccactttc
    13861 cgcgctttaa gagataaaaa atttgccgcc ctgcttttag gccagacctt ttcaaggatc
    13921 ggtgattttc tgtttcagat cgcgctggcc tggtgggtgc tggaaaagac aggctctgcc
    13981 accgccatgg gcacggtttt gttcttcagc acattaccca tggtggcttt cgtactggtc
    14041 ggcggcgtgg tggtggatcg gatgcggcgc ggcttgttgt tgttcatttc agacacggcg
    14101 cggtgtttca tcatggtcat tggggcatgg ttggccttca cagaccagct tgttctttgg
    14161 gagatctatg tcattgccat tctcttcggg tttgccgatg ccttctttct ccccgcctat
    14221 aacgcgctca ttcaacagat cgttgccgaa gaagacctgc ccagcgcaaa ttcaatcaac
    14281 agtttgagca tgcaattcgg gcgcgtggca ggcccggccc tcggtggatt gatcgccggt
    14341 ttcggtggaa ccgccgtcgc atttggattc aatgccctgt cctttttcat cggcgcattg
    14401 acggtcattc cgcttcttaa cctcccggtg cctgcttctt cagaagaaag cggttttacc
    14461 ctccagcatt ttaaagacga cctgcgcgac ggcttcaaca ccatcttttc cacccctgta
    14521 ttgtgggttt cgatcattgt cttcgcattt gcaaacatca ccctggcagg cccctacagc
    14581 attgccatgc cttttctcgt tcaggaaaaa ttgggcggcg acgaaaaaat gctgggcttt
    14641 atttattcca ttttccccat tgggtatgca atcgccagcc tggtcatggg cggttttcga
    14701 aagatacgat atcgcggaat cctgctctac gtttgcggcg ccattgcagg gatcgggttg
    14761 ggcgtgttcg gtttcggtct cccgctttat ctgctggtca ttgcagcatt gatcaacggt
    14821 gcagccctcg aaattgacgg cctcatctgg accaacctca tgcaggaaaa agtgcctccc
    14881 gaaaaaatgg gccgtgtctc cagcgtggat tcgcttgggt cgttcgtgtt gctccccatt
    14941 ggatacgcct ttaccggctg gctcatcgac accatcggtg tgtcatccgt ttttattgcc
    15001 gcaggtgtgg tgacagcgtt ggtcagcctg ctgccattgc tgcacccaac gattcggaaa
    15061 ttagactagc cgcacaattc ctaagcaaac tatcatctgc atataagaaa gtttttttag
    15121 aatggggaca tcatacgagt taacctgaac actcgtattg aatttgattt ccatgaatta
    15181 tccgcgccaa cttatgagga gatttaaacc atgaccgatt tatttaaaac cgtcccgatc
    15241 cgctcttccg agatcacgcc gaagagcgtg tatctttcac gtcgtgattt tatgaaagcc
    15301 gccacattca ccacaggggc ggctttgttg accgcctgcg ctccttccgt ggccggagct
    15361 gccgaggaca atgcacccgt cccaacatca aatgcgaccg atgaattggg tgacccggtc
    15421 aattcctata ccgatatcac caattacaac aactactacg agttcaccgt cgacaaacaa
    15481 caggtggccg atgaagcaag aggattcaac ctgaccccat ggaccgtgga agtgggcggc
    15541 ctggttaaca accccaagac gtttggcatc gaagacctgt tgaagttatt cccgcaggaa
    15601 gaacgcattt atcgcctgcg ttgcgtggaa gcctggagca tggtcattcc gtggacgggt
    15661 tttacgctgg ccagcctgct cgaaatggta gagccgactt ccgatgcaaa atatgtccgc
    15721 tttgaaaccg tatatcgccc cgaagaaatg cccggacaaa aaagcccatt ctatccgtgg
    15781 ccctatcagg aaggactgcg gctcgatgaa gccatgaacg acctcaccct gctagccaca
    15841 ggtctctatg gtgaacccaa cgtggcacag aacggtgcgc cgatccgcct ggtagtgcca
    15901 tggaagtatg gattcaaatc catcaaatcc atcgtgaaga tcgaactggt ggccgaagaa
    15961 ccatccacac tttggagcaa catcgcatcg aacgaatacg ggttttatgc caacgtaaat
    16021 cctgatgtgg accatccgcg ctggtcacaa gcctccgagc gccgcattgg cgaactcaac
    16081 cgtaggccca ccctgccctt caatggctat ggcgaacagg ttgcgcacct ctatgacggc
    16141 atggacttga gcgtgaatta ctaaccagag atgaacagag ataaacggag atcgcaacag
    16201 catctccgtt tatcttcggt agacacttca tgaaaaaatt cccctacaca ccgttacaga
    16261 tcgccgttca cctgtatgca tggtcagggc ttgccctttt gctgctggat tatttcacag
    16321 gtaatctttc ccccaacccc attcaagacc ttgaacagcg cacaggcagg cacgccatta
    16381 cattacttgt gttgtcactg ctatgcactc ccatcaacac catcttcaaa tggagcgaac
    16441 cgatcaaacg ccgccgcgca ctcggtgtgt acgcctttct atatgccacc gttcacatga
    16501 tcatcttcgt tgaccttgac ttcgggctgg cctggagcag cgttatcaaa gaagtcatcg
    16561 aaaagccgcg tctgatcgtc ggtgttatcg ctttcatcct gctcattccg ctggcgctca
    16621 cctcgttcga catctggaaa aaacgcctcg gcaaaaactg gaaacgcctg caccagctcg
    16681 tctacctcat cgccccgctg gtcttccttc attatgtttg gagcaaaaaa ggcgacctgc
    16741 ttttattaca gggagaaatc cttaaaccac tgatctacgg actcatcatc ggaatttttc
    16801 tggtcctccg cattcccccg attcgaaaag ccgtcgcctc tttttcaacc cgcagcctgg
    16861 ccctgctcaa caaaaagaat ccacaaccgg aagtggatac gccttaatac aaagacccca
    16921 aaggttttga aaatctttgg ggtctctttc tctaaaaaat tccgcgcagc agcaacagca
    16981 gaccgataat tgccagcgga atcccaatga tcgcaccgat caccgtcagg ctgacgatac
    17041 cgccaacgag gatcagcacc aggccaagca ccatcgcaac gaaacggcct gtcaattcca
    17101 cgatcacggc aagcagtttc cacaatgccg caaacggcca caaataccaa gggatgtgtt
    17161 gttttgtttg agtagtcata agtacctccg ctttgtttta cgcaaaacac gccgaaacga
    17221 tgcaagattg caaattcaat ttgctttata atccatctca tgaaaatgaa gtccaaattt
    17281 aataaatatt ggttgattcc catcgtgctg ctgcttgccg ttggcattta ttacctgccg
    17341 cccgttcaca gccgcctcgc ctggcgcgtg gatgccctgc gtgtgcaggt tatttatttc
    17401 ttcaacccgc ccgatgaagt ggtcttcgaa cccagtggtg atacttctcc gcttaccatc
    17461 gaaacagcca ttgccacagc ccgcgctgaa tacatgctga ccctcacccc gcaatataca
    17521 ccgaccgtca ctcccactac agggccgaca gccagaccca cggcgacatc cacacccttg
    17581 ccggaagcgg tcaatttgga aggggttgtg tacgtagacc agcacgaacg ttggaactac
    17641 tgcggccccg ccaacctgac catggcgctt aacttctggg gctggaaagg caaccgcgat
    17701 gacgtagcca agatcgtcaa accgggctcg ggcgatacaa aagtcaactt tattgaccgc
    17761 agcaaagacg ataaaaacgt catgccctac gagctggtgg atttcgtcaa cgagaacacc
    17821 gaataccgtg cgctcatgcg ccacggcggc gacgaaaacc tcgtcaaacg cctgctggcg
    17881 gcgggtttcc ccgttgttgt agaaaaaggt tattacgaac acgacaccac cggcaagatc
    17941 agctggctcg ggcattacct cttcaccacc ggctacgatg atgcccgcgg cggattcatc
    18001 gtacaagatg cctacaccaa acccggtaaa aatcttctca gcgattatgc gacctatctg
    18061 gaaggctggc gtgctttcaa ttatcaattc atcgtggtgt atcccgcgga gcgcgaagcc
    18121 gaagtgttaa ccctgttggg tccatgggtg gacgaaaatt gggcggccca acatgcgctt
    18181 gaactcgccg aaaaggacgt tcaaagcatc aacgataaca atctgttctt tgcctggttc
    18241 aacaaaggca ccagccatgt cctcctaaaa caatatgtgg atgccgccgc ggcttacgat
    18301 caggcatttt ccacttacgc caactgggat gaaaaagaac aggaacgtcc ctaccgcatc
    18361 atgtggtatc agacgggacc ctatttcgcc tacttttatt ccgcccgcta tgccgatgtc
    18421 atcagccttg ccgacacaac gctcttcgac cccgccaaca aaaaaggccg cacactcgaa
    18481 gagtccctgc tttggcgcgg acgtgcctac tatatgagcg gcaataccca ggccgccata
    18541 gatgattatc gcgccgcatt gcggatccac gtgaactggc cgcccgccgt acaggcgctt
    18601 caagacctcg ggctacagcc gtaatgggtt acaagttggc aaggttacag gttggaaggt
    18661 tcaacctgta acctgcaaac cttccaacct gtaaacacat attagatgga gttgaatgag
    18721 agtcttacat ttcgccgacg cacatattga catggccaac tatggccgcc acgacccgca
    18781 gacaggcctg cccatgcgtg tgcttgattt cctcaagtca ctcgatacga tcgtggatac
    18841 ggccatctct caaaaagtgg acctggtcat ctttgcgggc gatgcataca aagaccgttc
    18901 tcctgcgcca acctttcaac gcgaatgggg caggcgcatc atgcgcttat cacaggcgca
    18961 aatcccaacc ctgttattga tcggaaacca tgatatttcc ccggcggtgg ggcgcgccca
    19021 tgcattgcag gaattcaaga ccctgcaagt tccgttcgta aaagtgctgg accagcccag
    19081 ttttctcaaa cctgaagacc tgtgggaact gcccatgcag gtcattgcca tgccgtgggt
    19141 cacacgctcg gggttgatgg ccaacctcga aatgagcgga acagaaaccg agcagatcta
    19201 ctcgaacatc gaagagcgga tcgccagcct tgtggacgaa tgggtcagtg aagccgataa
    19261 aaacctgccg atcattttca cagcccatgc ctccattgaa ggcgcaaagt tcggcggcga
    19321 gcggcttgtg atgcttggca atgacctcgt gctttcaggc agtttggtga agaaccagag
    19381 attcaattat gtggcgatgg gtcacatcca caaaccgcag gatgtgaacg aaggccatca
    19441 gccgcctgtc atttacccgg gctccattga acgtgtggat tttggcgaag caaaagaaga
    19501 ccgtttcttt gtcattgccg agatcgaaag cggaaaagaa acgcaggtgg agtgggttca
    19561 actcacaggg gcgagaaaat tcatcgaccg ccgaacagtt ctcaggtcca acgagaacgt
    19621 gaccgaggct cttaaagacg cattgcccag cctgaaggag atgtctgagg ccattgtccg
    19681 cctgtcggtg gaatatccaa gagaatggga cacattgatc gatgaaacgg ctttgcgaaa
    19741 atatactgag gatgcctttg agttccatct ggtaaaaaga ccgcaaagcg aagcgcgggt
    19801 gcgcattccc gaaggtcagg tggtgagcag tttgagtccg ctggacctgc tggcacaata
    19861 cttcgatgcc gccaaggtga aagatgccga tgaactcaac aaacttgcgc aggagatcat
    19921 ttcagaagac acgcttgaaa actgaaacct gcctcttgac acctgacacc tgacactgga
    19981 tactagggca tcctatcgcc gcgaggtatt catgtcaaaa gagaattcac ggtatcgctg
    20041 gttcgtcgtt gttgttttct ttttcttcat gctcctgcac cagaccgaca aattgatgat
    20101 cggttccatg caagtggata tcagcaagga ctttaatctc aacaaccaac aatggggttt
    20161 gatcaattca ggggctctga tcgttgccac gatcctgtat ccgatctggg gatatttata
    20221 cgaccgctat tcacgcacaa aattgctttc actggcctcc ttcatttggg gtgcaaccac
    20281 ctggctcaat gccatcgtaa aaacctttgg cggattttta accacgcgcg cttccaccgg
    20341 cattgatgat tcatcctacc ccggcctgta caccctgatc gccgattatt tcggccccaa
    20401 cctgcgcgga aagatctacg gcattctgca actggcccag cccatcggct atctggttgg
    20461 gatgatcctg gcgttgatgg ttgcgcctgc cattggcgga tggcgcagtg ttttctacat
    20521 cacaggctcg ttggggttgg tcatcgccgt gttgatctat ttcggcgtga gagaaatgcc
    20581 gcgcggacag gccgagcctg aatttgagaa tatgcccgaa atgcagacct tcaaattctc
    20641 atgggccgaa gccagagaga tcttcaaaaa gaagaccatg tggttcgttt tcctgcaggg
    20701 ctttgcgggc gttttcccgt ggaatgtgat caccttcttt ttcttcgcct atcttgaaaa
    20761 agaacgcggc tacgatgcaa acggcattct cttcacaatg gcacccgtca tcctcatcct
    20821 cgccagcgga tatttcatcg gaggtgcgct cggcgacatg gccttcaaac gctcgaacaa
    20881 aggccgcatc gtagtttcca gcatcggggt tctgcttggc gcattgttca tgtatcttgc
    20941 catcaccaca cccgtagacg ccaaaaatca attcttcatt ttgatgtgcc tcaccgccgt
    21001 ttttatgccg ctttcatcgg caaatgtgat cgccacggtt tacgatgtca ccgtacccga
    21061 ggtccgctcc actgcacagg ctgtggaata cttcattgaa aatgcggggg cagcctttgc
    21121 ccccatcctc acagggtaca tcgcagatct ctacgatctg aaaaccgcca tcctgatcat
    21181 ttgcacagca gcctggcttt tgtgtttcgt cttctacctc ggcgcgatct tcaccatcga
    21241 caaagattca cacgacctcc gcgaacaaat ggcagcccgc gccaaatcca tgtaagcctg
    21301 agtcaggcca aaccgcctgc ttttttggat gagaatccgt ttcagcctta acctgaagcg
    21361 gattctgttt tacggacagt ttctccccgc attttttatc gcagcaaatc ttcggcgacg
    21421 attctgcctg ggtgggcacc ccggccgcag gtccgctttg gatgcggaaa tgtcgtgaca
    21481 atcgtcactg aaagcggaaa aagtccgcat gttataatcc gcataaattt tcacaaacca
    21541 aaagaggaag acatggcata cacaaatgta aaagtccctg aaggtggggc aaagatttcc
    21601 attcaagatg gcaagttgaa cgtacctgac aatccaatca ttcccttcgt tgaggg
//
LOCUS       DAOQJC010000063        21540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1101_length_21540_cov_9.936281, whole genome shotgun sequence.
ACCESSION   DAOQJC010000063 DAOQJC010000000
VERSION     DAOQJC010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 21540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21540
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1101_length_21540_cov_9.936281"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1944)
                     /locus_tag="PLE39_10945"
     CDS             complement(16..1944)
                     /locus_tag="PLE39_10945"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNE68891.1"
                     /translation="MISRIRVGIKYLFQLGFRPLALYGLYKFGLKTGHYRRITPVTAD
                     TITPNSHSLFVLPDRDLLAQILGEDGKTDLLNAADEIAAGKVRVFGELTPLDFKLNQP
                     LRHWTEYESKPALLSSFFFLHNDIKFLWEPARFGWAFILGRAYHLTQDEKYADAFWRH
                     FEAFTDANPVNMGPHWMNGQEVAIRMMALVWCAHVFAKAPIANYQSLVTAIYQHAARI
                     PSTLVYARAQNNNHLITEAAALYTAGLFFKNKTLRESGWHWLNWCFQNQIGDFGEYIQ
                     HSANYHRLMLQTALWVNLIKQDVFSAHTSQAIARSAHWLFSLLDNVSGLTPNLGANDG
                     ALIFPLSAADFRDYRPTVQAASRAFLRTSLPSGMWDEMSLWFGLPANERVINSDAYLT
                     ENVRGKNSWAYLRASRFRSRLSHMDQLHLDLWWRGLNIAQDAGTYLYNAESPWDNPLV
                     TTRVHNTVTVNGRDQMTRAGRFLVLDWINAYSKNLIEADDEILGRTRAYHRAYHGLKH
                     ERTVTVYQNERWVVNDKFSARSRSERTYRLQWLLPDYEWELENQDDAHGIRLRIKSPS
                     GWITLAVHSSFPLQLSSFSLVRAGELVYGQREVQPYEGWVSHIYGQRAPALSFVVEMT
                     SAYDTELTSEFIFPDSAA"
     gene            2133..2375
                     /locus_tag="PLE39_10950"
     CDS             2133..2375
                     /locus_tag="PLE39_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNE68892.1"
                     /translation="MEELISKLAAFIATDILKQPKRAIRADEALISSGLIDSFSLMDI
                     SLFVEGNFGVRIDDTELNAETFDTLEQLAKLIQSRQ"
     gene            2379..3830
                     /locus_tag="PLE39_10955"
     CDS             2379..3830
                     /locus_tag="PLE39_10955"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE68893.1"
                     /translation="MDLSSPLFLFLFLPVFLLIYLVSAGKARTGILLLASVIFLAWGD
                     ASAILWLGGILVSGYWMGLAIAKAQETGQSAAKWLWAGLGVNVILLAFFKYLTAFGQE
                     GLLWLNTPETWVDDLSGLTVPIGLSYVTFQLIAYLVDVWKGNTPAEKNFVSFSAYLLF
                     FPKLVSGPITRYKPFAVQMEQLNPSMDDVAAGFRRLLSGFIKRTVIANQLALMANAVF
                     NQPTANVEPKFAWLGLAAYTLQIFFDFSGYTDMALGLGLMIGIRLPENFNFPYIAQSV
                     SDFWRRWHITLSTWFREYVFYPLERKRFKWMGQQVNILIVFLLTGLWHGFNPTFMVWG
                     MIHGIALAVESLGFGRWLKGAWRPLQHLYTLTIVVIGWVFFRSQDLNFAFEFFRRLAG
                     DRSNLMPLPFSQTTPIPFIEPSFIMALIAGTLFSFPLTSLWRQLRERFESRQSNSYLL
                     FQVMEDAALLLFFILGLGLQLSGSFLPNLYAKF"
     gene            3844..4911
                     /locus_tag="PLE39_10960"
     CDS             3844..4911
                     /locus_tag="PLE39_10960"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68894.1"
                     /translation="MSTESIARQRYSLAFVLFLLGGLLFIMISSSLAGISDFENNLIM
                     KNLLIEKTNWLHLKIGDRVFPEVVIGKDGWMEYSGDGNLDDFQTMQPLWNKHSLAKRL
                     TAFEAYLNEQGITLLIVVAPNKASIYPERLPAEIQPLTEQTRLDDFIAALKENNLPQV
                     LDLRPALQQAKLEQEVYYYSNTHWNGYGAFVAYTTILNELAKTHPELHPYTPEELDLG
                     TKRATVQDIAKLLQANFIKEPHFFYIPQGHPVQSLTLKDYYGFNHITSVDDESLPTLL
                     MIHDSFGYRFLNEYMSMNFEKAYFIHSGKDTPTYLNRAAIEQFNPDIIVIEIVERSLN
                     NLNNYLSQFDSPIKFYQEPKK"
     gene            4908..6590
                     /locus_tag="PLE39_10965"
     CDS             4908..6590
                     /locus_tag="PLE39_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE68895.1"
                     /translation="MTTLADLIHQNYSKNPDKTAIILQHAGQDDKPVSYLDLVKGSAQ
                     YAQTYAEAGIQPGEVVILILQHGEDLVYSFWGAILHGAIPAIMPFLTEKLSPERYRAD
                     LTALLSVTQPAAIVTYPEFEADIRAALTADGSVRRVLLTSQLAPRSEPDFNQLRGLQC
                     KPTDIVLLQHSSGTTGLQKGVALSHQAVLNQLNAYTRAIQLTSDDVLVSWLPLYHDMG
                     LIAGFLMPILMGITLVLQSPFDWVRAPHKLFQSVSKYKGTLSWLPNFSYNFCAQKVRE
                     RDLEGVDLSSWRVITNCSEPVKWESHQSFFQRFEKYGLRESALNCSYAMAENVFGVTQ
                     TPLGTKPVTDEIDRASYINERIANPPQDGKPALKMMSSGRALPNTRLLILSEEGKPLP
                     DKVIGEIALQSDCMLTGYYHRPDATEKALRDGWYLTGDYGYTVNGEIFVAGRKKDMII
                     VGGKNVYPQDLESLAYEVPGIHAGRAVAFGIEDTKAGTEEVVIVAEVDTDDPEERQRI
                     ADALRKHITQNSAIALRHAHLVDPKWIVKTSSGKTARSANREKFLKEMDMQS"
     gene            6633..6899
                     /locus_tag="PLE39_10970"
     CDS             6633..6899
                     /locus_tag="PLE39_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68896.1"
                     /translation="MSVLIAVLTIFVLVINYNLYQIWFNPKDYIDKLTYNSLPFLMLN
                     NPRLYKWYTSKTFLWFARILNLFFALLMTTFLFLVVLGSLGLFP"
     gene            complement(6983..7573)
                     /locus_tag="PLE39_10975"
     CDS             complement(6983..7573)
                     /locus_tag="PLE39_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68897.1"
                     /translation="MSFDEPNFDDAGDAPLPEESNNNRTFLIAVGILGGIILISIACL
                     GAVYVFGSQGRAAVQETEVAGAAMASATANAVNETVNRAITATFEASILPTATTTFTV
                     TPTSPVGVASATPTPDPALAGLSGTSVAQTATIGAAYTQAALAALSTVPPTSTALPNT
                     GFADEFGLPGIVVMSLAFVIVILLARRLRSAPETIR"
     gene            complement(7634..8689)
                     /locus_tag="PLE39_10980"
     CDS             complement(7634..8689)
                     /locus_tag="PLE39_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68898.1"
                     /translation="MQAPVHHIVGLTSIVRERLLPVNGTVLARLNQKVSPSDVIAEAK
                     WSREHVLLNVAHQLRVKPEAADRLIKFNFKDVVPADAEVAVGEGLFPRVVRAPREGRV
                     AAVGGGQVLLEVGESKIELRAGIAGTVIEIVPNRGVIIQTAGALVQGVWGNGRIDSGL
                     LVNLADKPESVLTADRLDVSLRGSIILAGMVKDAETLQAAAELPVRGLILSSLYPSFI
                     QLAREMRYPIVVTDGFGPMPMNSAAYRLLSTNAKRDVTVNAEVYDRYSGARPEVIINL
                     PISSDPPAPREVETFSPGLQVRMRRPPAMGAIGVISAIKSGLTKLPSGLHAHAAEVKL
                     ENGETVIVPLVNLEVVG"
     gene            complement(8694..10451)
                     /locus_tag="PLE39_10985"
     CDS             complement(8694..10451)
                     /locus_tag="PLE39_10985"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HNE68899.1"
                     /translation="MASSLIDGNSILAIDVGAANTRAVFFDVVEGEYRFVASGVAPST
                     AEAPYKDVSEGVRNAIQNLQSVLGRSLLDGSRNLITPSQPNGSGVDALVATLSAGPTI
                     KTVLVGLLKDVSLESARRLAESTYMRIVETLNISDRRKPDQQIDAVLRARPDLIVIAG
                     GTDGGASRSIQKLLEPIGLASFTMPEEKRPAVLYAGNEKIEAEIKTLIGSLATSLHFS
                     PNVRPSLDTEDLEPAQRELARMTINIRKRQIRGVDLLDMWSGGHLLPTAYATGRMVRF
                     LSKVYSSQKGILSVDLGASAAIIAAGFSDKSSLNVYPQFGLGENLTGLLNYTKLEDIL
                     RWSSLDVSPGVLRDYMYQKALYPSTIAATKEDLDMSQAIARQALFLAMQSARRDFPRN
                     VAGIKPNLTPLFEPILAGGGILSDASRPGQSLLLLLDGIQPVGVTTVIIDQNNLLPLL
                     GVTASQNNILPVQVLESGAFLSVGTVVAPVTSAAYGTPILRAILTYENGTEARMDLKY
                     GGLETLPLANGQTGKLTVQCLRGTDVGFGPGRGGTIPVSGGALGVVFDGRGRPLDLPA
                     DSVRRRDLIKKWNWTLGGE"
     gene            complement(10454..11167)
                     /locus_tag="PLE39_10990"
     CDS             complement(10454..11167)
                     /locus_tag="PLE39_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68900.1"
                     /translation="MVLTPQMLDMITGALSLLFTILIFSYVLGDNPLFRVAVYIFVGI
                     SAGYIASVAIWQVIWPKLLYPLVVGPSTPFESIGYLVFPLLGFALILMKISPRLAGLA
                     RFVMAFIVGVGAAVAIAGALSGTLIPQVSGAINSFDMVAAGARNVGFIEALVNGGTIL
                     AGTIFTLIYFHFGARPKADGTMHRFALIEIPAWIGRLFIGITLGVVFAGVYSAALTAL
                     IERIDSIINFINSFIGNFQ"
     gene            complement(11169..>13013)
                     /locus_tag="PLE39_10995"
     CDS             complement(11169..>13013)
                     /locus_tag="PLE39_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68901.1"
                     /translation="PVSSSNDLFGSATTDDTPDWLRGMQAQSAEPAASSEPVSSGFPE
                     SSADLFGGNVPDWLKGTAEEKSTAAPLSEPDWLKSESENTPVDNAPDWASAGVTSGDE
                     LEMPAWLNPAESAAQPSEAQDISLGDVPDWLKASAPKETIFGETPAADPAAVPDWLNS
                     LQSAAPESSSKPAEIEFSSTPPAEAGIDNLFTDLPDWLSKADDMAAPVSSSPSPITNT
                     DAIAPGDLPSWVQAMRPVDSGLPTSMPVSLSSDQTLESRGALAGLQGVLPAVPGFSPA
                     SKPKAYSIRLQANEEQQSHAALLEQILAAETAPVPLSSLSNLNTSRALRWFLVAVFFL
                     ILPAILIMRTQFFALPPSELKPAMKGALDVAQLLPEGAPVLVAFDVEPARVGEMEAVA
                     APMFDQMLLLHHPRFTFVSTSESGAILAERFISTGPLAGHQYQSGIEYMNLGYLPGGQ
                     MGIYSFAQNPRQSAPYLFNSGVTESPLLNAYLSPAWAAEPWSGITTLSQFTAFVIVTD
                     NADSARAWIEQTSSTRGAMPIVVISSAQAAPMIRPYFDSQQISGLVAGLYDGAVFEQN
                     NLNRPGTTRIYWDAYNIGLLLAMVLILLGGLWNLVLGLRDRAAAREVK"
     assembly_gap    13014..13113
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13114..13939)
                     /locus_tag="PLE39_11000"
     CDS             complement(<13114..13939)
                     /locus_tag="PLE39_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68902.1"
                     /translation="MADMIKCPVCGENNRLGQEFCLNCQTRLGPVPGSSKGDETLKPG
                     QAPTKKNTADLEPILPQWLKDARDSARNIGSGDVPAEPSTPKQTQPASPVDFLAGLHS
                     QADDNDEEDIPDWLANITGDAPKSKKSQPEPSEVRWVEMGEKNDFVQDEQPAAESDTP
                     PWLAGLASNAPQTEKDELTDWFRDASGIQKPAEAAPSSSVDSPDWLRSMAAEDGQGGF
                     TSSSEPADTFGNISASADDTPDWLRAMAADNEAKNNSQPASSSNDLFGASSADDTPD"
     gene            complement(13939..14556)
                     /locus_tag="PLE39_11005"
     CDS             complement(13939..14556)
                     /locus_tag="PLE39_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68903.1"
                     /translation="MRIFSVIFAFIAITAGVLVLLGYFIPAFSVIQVLLLNWAVILAG
                     VAVIVGVFNLALVHSDKVRRGEKGSVYSALLVVSLLATLIFGIFLRPGHTIMKMVVNG
                     IIIPSEAALMGLLTISLLYAAIRLLRRRVDLMSIIFLITAAFVLLGTATLTFGGIPLI
                     GEASQWVTQVLALGGARGILIGVSLGILMTGLRVLFGIDRPYGGN"
     gene            complement(14556..15293)
                     /locus_tag="PLE39_11010"
     CDS             complement(14556..15293)
                     /locus_tag="PLE39_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68904.1"
                     /translation="MKIIELAILAVSALLLLAVTFWKRRSPANLRDIPALTRLARTLG
                     LSIEDGTRLHISLGRGSFLDVRGGSGLAGLAVLRHIAERTSVSDEPSIASAGDPALGL
                     LAQDTLQAGYHAAGVEELYVPTTGRVTGLTPFGYAAGAMNISQHENVSTNILLGHFGP
                     EVALLAETADRENITVIGASDDLTGQAVIFANTQDALIGEELFAAGAYLGAGVSHAAS
                     LTVQDILRWFIILALMGGAFAKFAGMI"
     gene            complement(15290..15529)
                     /locus_tag="PLE39_11015"
     CDS             complement(15290..15529)
                     /locus_tag="PLE39_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68905.1"
                     /translation="MKPSREYWPRWAETLRRYQLTEFAASLLEAGSPLALLGAQALYF
                     SRGLFESDQLTALAATLEEDGQAFASYLVEERASL"
     gene            complement(15615..16865)
                     /locus_tag="PLE39_11020"
     CDS             complement(15615..16865)
                     /locus_tag="PLE39_11020"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNE68906.1"
                     /translation="MPSLNEVLAVILGGGRGARLYPLTQMRSKPAVPIAGKYRLIDIP
                     ISNCINSGIYRIAVLTQFNSVSLHRHITNTYHFDSFHPGWVQIWAAEQTIESADWYQG
                     TADAVRKQTLEIQSSGAKNVLILAGDHLYRMNYNEMAEYHWNSKADITVAVQPVPRDE
                     ASRLGILKREKNGRISAFVEKPKDVEVQNKFISRDDAVRPFLGSMGIYMFKTRVLMEL
                     LSNFPSFHDFGGDIIPQAIQSYSVYGFDFSGYWQDIGTIRSFYETNLALTTSETPFNF
                     YDPKLPIYTDTRFLPGSIVEDSKIRDVLIAEGSRIQKSDITHSIIGIRSQISRGCIIK
                     DTIMLGSDYYERDDEGLPIGIGENCHIEGAILDKNVRIGSDVVIRPFPRNTDIDNAKW
                     YVRDGIVVIPKDMVIESGTKIGPE"
     gene            complement(17354..17436)
                     /locus_tag="PLE39_11025"
     tRNA            complement(17354..17436)
                     /locus_tag="PLE39_11025"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17400..17402),aa:Leu,seq:caa)
     gene            17490..18548
                     /gene="ychF"
                     /locus_tag="PLE39_11030"
     CDS             17490..18548
                     /gene="ychF"
                     /locus_tag="PLE39_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNE68907.1"
                     /translation="MKLGIIGLPQSGKTTIFNALTRSNTPTSASAGRIEVHTAVVDVP
                     DPRVTKLSGMFNPKKTIYAKVTYADIAGLESGSAKSGISGQLLNQLNQMEGLILIVRG
                     FADDNVMHPNGSIDPLRDVDSMLTELLLNDMIAVERKLEKLTEERKKGGTDKVINARQ
                     TELFEKLHATLSDNKPLRSMEFTNEEQKELASYGLLTRKPILTVFNMGEGQTAPTAQL
                     DHPSVALMGKLEMEIAQLSAEDAAVFMEEYGIKELSLNRMINLSYDLLKVQTFFTVGE
                     DEVRAWTTRRGASAAESAAEIHTDLQRGFVRAEVVSYDDLTSLGSMNEAKAKGKLRLE
                     GKEYPVKDGDIMHIRSSL"
     gene            18632..>19587
                     /locus_tag="PLE39_11035"
     CDS             18632..>19587
                     /locus_tag="PLE39_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68908.1"
                     /translation="MFRERGVWGAILLSLIFLFVLACGISVTPNTSSPPAFDPTKAAL
                     ELQATSMSLQLTQAALNAQQAAQSTSPPPQPTAAPTEAPAQAQPTPTQDVEARIRAAN
                     VLVYENTDEYGIGMWIQDALDNMGIKYTQTGSYSGHFMEYLNSGTKYDLIIVGAEAKD
                     RISGEFWDVINTRLTRDKVALIAEVWYLDTEANGPISKILGGCGIRYQKDYQLADSIY
                     WWDSTHPVFNEPNTVLPLLHYDRYWANQTGDQIRLGGSGDAVLLAGLAAQKSNEEAVL
                     ATCYEGRVIIQTFSDHDYKMADVIPLWENYIHYTLKNHFLAVP"
     assembly_gap    19588..19687
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19751..>21540
                     /locus_tag="PLE39_11040"
     CDS             19751..>21540
                     /locus_tag="PLE39_11040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNE68909.1"
                     /translation="MAYTQKKWSLAPLYPGYESAELQNAFDMIEEQVTSFEGVRGKLN
                     PEISAEQFMQIVRASEETLRIANKLYSFAGLSFTADTQDQAAQSLQNRAMQFLAEIEN
                     RTMFFNLWWKEVDETNARRLMDAAGDYRYYLEALRLFKPHTLTEAEEKIVNIKNVTGS
                     NALINLYDAITNRYTFKMRVGGKEKEMTAAELQSFRYSTDPKVRAESYQEQFRVYGND
                     GVILGQMYQTRARDWHNENIDMRKFTSPIAARNLNNDIPNEAVDALLNTARKNVKIFQ
                     RYFNLKAKHVGMKKLRRYDIYAPVARSKKTYEFDTAADMVLEAFSAFDPKVAELAKRV
                     FDEDRIDSEIRKGKRGGAFCWSVLPEMTPWVLVNYQGTGREVATLAHELGHAIHSMLA
                     AGHNTFSFHSSLPLAETASTFAEMMLIDKMLAEETDESVRRDILFKQMDDAYATILRQ
                     SYFALFEKQAHEMVQKNASVDELCAAYMENLKEQFGDSVELSDEFKWEWVGIPHIYHT
                     PFYVYAYAFGQLLVLSLYQQFKAEGESFKPKYLKILSAGGSEAPEKILTEAGVDIRSA
                     KFWQGGFDVLEKLVSELEQLPADKPKVKKAV"
BASE COUNT         5542 a         5727 c         5371 g         4700 t
ORIGIN      
        1 cttgaaaata ttttatcacg ccgctgaatc agggaagatg aactcgcttg ttaattcagt
       61 gtcataggcg gaagtcatct caactacaaa cgacagcgcg ggagccctct gcccatatat
      121 atgagacacc cagccttcat acggttgaac ctcccgttga ccgtaaacca attcaccggc
      181 acggacgagg gaaaatgacg aaagctgaag ggggaaggat gaatgaactg cgagtgtgat
      241 ccacccagat ggagatttga tacgaagcct gattccgtgc gcatcgtcct gattctccaa
      301 ctcccattcg taatcgggga gcagccattg taagcggtag gttcgttccg agcggctacg
      361 cgcagaaaat ttatcattta ccacccacct ctcgttttga taaactgtca ccgtgcgctc
      421 atgtttcaaa ccatgataag cccgatgata ggcccgtgtg cgacctaaaa tctcatcatc
      481 cgcttcgatc aaattctttg agtaggcatt aatccaatcc aacacgagga agcgacctgc
      541 acgagtcatt tggtcgcggc catttacggt gacggtgttg tgaacacgcg tcgtcaccag
      601 cggattatcc cagggcgatt cggcgttgta caaatatgtc cctgcatcct gggcgatgtt
      661 taatccgcgc caccacaggt cgagatgcaa ttggtccatg tgcgaaaggc gcgaacgaaa
      721 acgcgaagcc cgcaggtacg cccatgaatt ttttccacgc acgttttctg ttagataggc
      781 atcggagttg atcactcgtt cgttggcggg caggccaaac cacaaggaca tttcgtccca
      841 catgccagag ggcaggctgg ttctcaaaaa agcacgcgat gcggcttgca cggtcgggcg
      901 gtaatcgcgg aagtcggcgg cgctcaaagg gaagatcaat gccccatcgt tggcacccag
      961 gttcggggtc agccctgaaa cgttatcgag gagagagaaa agccaatgag cagaacgtgc
     1021 aatcgcctgc gatgtgtgtg cggagaagac atcctgtttg atcaaattga cccacagggc
     1081 cgtttgcagc atcagccgat gataattggc gctgtgctga atgtattcgc caaaatcgcc
     1141 gatctggttt tgaaaacacc agttgagcca atgccagcca gattcacgca aggtcttatt
     1201 tttgaagaac aaccccgctg tgtacagcgc agcggcctcg gtgatgagat gattgttatt
     1261 ttgcgcgcgg gcatagacga gtgtggaggg aatgcgcgct gcatgctgat aaatggcggt
     1321 gacaagagac tggtaattgg caattggtgc tttggcaaag acatgcgcac accagacgag
     1381 agccatcatt cgaatcgcaa cttcctgtcc gttcatccaa tgcggcccca tgttgacggg
     1441 attcgcgtcg gtgaaggctt cgaaatgcct ccaaaatgca tcggcgtatt tttcatcttg
     1501 cgtgaggtga taggcgcggc caaggataaa cgcccagccg aagcgggcgg gctcccagag
     1561 aaatttgatg tcgttgtgaa gaaagaagaa tgaagaaagc agcgctggct tcgattcgta
     1621 ttccgtccaa tggcggagag gttggttgag tttgaaatcg agcggggtta attcgccaaa
     1681 cacacgaact tttcctgcgg cgatctcatc ggctgcgttg agaagatctg tttttccatc
     1741 ttcgccaagg atctgcgcca gcagatcgcg atcggggaga acaaacaggg aatgggaatt
     1801 gggagttatg gtgtcagcgg ttacaggtgt gatgcgcctg taatgccctg ttttcaaccc
     1861 aaatttatac aagccataca aagccagcgg gcgaaagccc agctgaaaaa ggtatttgat
     1921 cccaacacga attctcgata tcatggttcc caaagtttac cagccaattt gcaaaagggc
     1981 ggcattcccc cgccactgat agtgtagaat tcggggctga ccgcagcaca catcaggatt
     2041 gatccctgct gccttcaacg cgaacaagcc aagaaaattc gtgaaaaact tgtacgataa
     2101 agaaattaca acccaaaaag gaaccattca tcatggaaga actcatttca aaacttgccg
     2161 ctttcattgc aaccgatatt ctcaaacaac ccaaacgcgc cattcgcgcc gatgaagccc
     2221 tgatctccag tggattgatc gactccttca gcctgatgga tatcagcctg ttcgtggagg
     2281 gaaatttcgg cgtccgtatc gatgatacgg aactgaatgc agaaaccttc gataccctgg
     2341 aacaactcgc caagctgatc caatcacgcc agtaattcat ggacctttct tcgccgctct
     2401 ttctcttcct gtttttgcca gtctttctgt tgatctatct ggtcagcgct ggcaaagcgc
     2461 ggacgggcat cctcctgctt gccagtgtca tctttctggc atggggagat gcttctgcca
     2521 ttttatggct gggcggcatt ctggtcagcg gatattggat ggggctcgcc attgcaaaag
     2581 ctcaagagac gggtcaaagc gcggcgaaat ggttgtgggc agggttgggg gtcaatgtaa
     2641 ttctgctcgc cttcttcaaa tatctaaccg cctttgggca ggaaggtttg ctctggctca
     2701 acacccctga aacatgggtg gatgacttga gtgggttaac cgtacccatc ggcctttcct
     2761 atgtcacgtt tcaattgatc gcctatctgg tggatgtgtg gaagggcaat acgcccgccg
     2821 aaaaaaattt tgtttccttt tccgcctatt tgctgttctt tccaaaactt gtctctggcc
     2881 ccatcacccg ctacaaacca tttgcagtgc aaatggagca actcaaccca tccatggacg
     2941 atgtggctgc agggtttcgg cgcctgcttt cagggtttat aaaacggact gtgatcgcaa
     3001 atcagctggc gctgatggcc aatgccgttt tcaaccagcc gaccgccaat gtggaaccga
     3061 aattcgcctg gctgggattg gccgcataca ccctgcagat cttttttgat ttttcaggct
     3121 ataccgatat ggccctcggg ctgggattaa tgatcggaat ccgcctgcct gaaaacttca
     3181 attttcccta catcgcgcaa agtgtcagcg atttttggcg gcgctggcac atcaccctct
     3241 ccacctggtt tcgtgaatat gtcttctatc cgttggagcg caaacgtttc aaatggatgg
     3301 gacaacaggt caatatcctc atcgtcttcc tgttaactgg tctctggcat ggcttcaacc
     3361 ccaccttcat ggtttggggc atgatccacg gcattgcatt ggcggtggaa agcctcggct
     3421 ttgggcgctg gctcaaaggg gcatggcggc cattgcagca tctctacacc ctgaccatcg
     3481 ttgtgatcgg ctgggttttc ttccgctcac aagatctgaa ttttgccttc gaattcttcc
     3541 gccgcctggc aggtgacagg tccaacctta tgccgctgcc attcagccag accacaccga
     3601 ttccattcat tgaaccgtcc ttcatcatgg cattgatcgc agggacactg ttctcgttcc
     3661 cactcacctc gttgtggcgg caattacgcg aacgattcga atcacgccag agcaattcct
     3721 acctcctgtt tcaggtcatg gaagatgcgg cactgctcct gtttttcatc cttgggctgg
     3781 ggcttcaatt gagcggcagt ttcctgccaa acctgtatgc aaaattctaa aatggaaaaa
     3841 gtcatgtcca ccgaatcgat tgcacgccag cgatattccc ttgccttcgt tttgtttttg
     3901 ctgggcggcc tgctttttat catgatctca agcagtctgg ctggaatttc agattttgaa
     3961 aataatctca tcatgaaaaa cctgctcatc gaaaagacca actggctgca cctcaagatc
     4021 ggagacaggg ttttccctga agtggtgatc ggaaaagatg gctggatgga atacagcggc
     4081 gacggcaatc tcgatgattt tcaaaccatg cagccgcttt ggaacaaaca ttcgctggca
     4141 aaacggctga cagctttcga agcctactta aacgagcaag gcatcaccct gctgatcgtg
     4201 gttgccccca acaaggccag catctacccc gaacgcctcc ccgctgagat tcagccgctt
     4261 accgagcaaa ccaggctcga tgactttatc gccgcgctaa aagaaaataa cctgccccaa
     4321 gtattggacc tccgccctgc cttgcagcag gcgaaactcg aacaggaagt ctattactat
     4381 tccaacaccc actggaacgg ctatggcgca ttcgtggcat acaccaccat ccttaatgaa
     4441 ctcgccaaaa cccaccccga actccatccc tacacaccag aggaactgga tctgggcaca
     4501 aagcgcgcca cggtacagga tatcgccaaa ttgttgcagg ccaatttcat caaagaaccg
     4561 catttcttct acattcccca aggccaccct gttcaatcgc tcacgctgaa agattattac
     4621 ggcttcaatc acatcacctc tgttgacgat gaaagcctgc ccaccctgct catgatccac
     4681 gattcgttcg gctaccgctt tctcaacgaa tacatgagta tgaatttcga aaaggcatat
     4741 ttcatccaca gcggaaaaga cacccccacc tacctcaacc gtgccgccat tgaacaattt
     4801 aacccggata taatcgtcat cgagatcgtg gagcgaagtt tgaacaacct caacaactat
     4861 ctttcccaat tcgattcccc catcaagttt tatcaagaac ccaaaaaatg accacgcttg
     4921 ccgacctgat tcatcaaaac tacagcaaga atccagataa gaccgccatc atcctccaac
     4981 atgcagggca ggatgacaaa cccgtttcct atcttgacct ggtaaagggt tccgcacaat
     5041 atgcccaaac ctacgccgaa gcagggattc aacccggcga agtggtcatt ctcattttgc
     5101 agcacggcga agacctggtc tattcatttt ggggcgccat cctgcatggt gcaattcccg
     5161 ccatcatgcc gttcctcacc gaaaagctct cccccgaacg gtatcgcgcc gatctcaccg
     5221 cgcttctttc ggtcacgcag ccagccgcca ttgtcaccta ccccgaattc gaagcagata
     5281 ttcgcgccgc actgacagcc gatggctccg tccgcagggt tttgctcacc agccaactcg
     5341 cgcccagatc cgaaccagat ttcaatcaac tgcgcggctt gcaatgcaaa cccaccgata
     5401 ttgtcctgct ccagcattct tcaggaacca caggtctgca aaaaggcgtc gccctttcac
     5461 atcaggcggt gctcaatcaa ctcaacgcct acacccgcgc catccaactc accagtgatg
     5521 atgtgcttgt ttcatggctg cccctgtatc acgacatggg tctcattgca ggcttcctca
     5581 tgcccatcct catgggtatc accctcgttc tacaatcccc gttcgattgg gtgcgtgcgc
     5641 cgcacaagtt atttcaatcg gtcagcaaat acaaaggcac actttcgtgg ctgccgaatt
     5701 tctcctacaa cttctgcgca caaaaggtcc gcgagcgcga tcttgaaggc gtggaccttt
     5761 catcgtggcg tgtcatcacc aattgctccg aacccgtcaa atgggagagt catcagtcct
     5821 tttttcaacg cttcgaaaag tacggcttgc gcgagtccgc gctcaactgc agttacgcca
     5881 tggcagagaa tgttttcggg gtcacccaaa ccccgctcgg aacaaaacca gttaccgatg
     5941 agattgaccg cgcgtcctac atcaacgagc ggatcgcaaa tccgccccag gatggaaaac
     6001 ccgcgttgaa aatgatgtca tcgggacgcg cgcttcccaa tacccgtttg ctgattctgt
     6061 ccgaagaggg aaagcccctc cccgataaag tgatcggtga gatcgccctg caaagcgact
     6121 gcatgttgac gggttattat caccgccccg atgccaccga aaaagcattg cgcgatggct
     6181 ggtatctcac aggagattac ggctacaccg tgaacggcga gatctttgtg gcaggccgta
     6241 aaaaggacat gatcatcgtc ggcggcaaga atgtgtatcc acaagacctg gaatcgctgg
     6301 cttacgaagt gcctggtatt catgcaggcc gcgccgtggc atttggcatc gaagatacaa
     6361 aagcagggac agaagaagtg gtcatcgtgg ccgaagtgga taccgacgac cccgaagaac
     6421 gccagcgcat cgccgatgcc ctgcgaaaac acatcaccca aaattcagcc atcgccctgc
     6481 gccacgccca cctcgttgac ccgaagtgga tcgtcaaaac ctccagcggc aagaccgccc
     6541 gctccgccaa ccgcgaaaaa ttcttaaagg aaatggatat gcaatcatag ttttaagtat
     6601 tgccaaagcc aactgacaaa atgagcaaac ccttgtccgt tcttattgcc gtgctcacta
     6661 ttttcgtcct tgtgatcaat tacaacctgt atcagatctg gttcaacccc aaagactaca
     6721 tcgacaagct gacatacaac tcattgccgt ttctgatgtt gaacaatccc cgtctttaca
     6781 aatggtacac ctcaaaaacc tttctatggt ttgccaggat tttgaatttg ttttttgctt
     6841 tgctaatgac cacgttttta tttctggtcg ttttgggaag cttggggctt tttccctgat
     6901 aaatggcgct tcacgataag ctgttaaacg aaaacgggat ggccgcataa gcgtgccatc
     6961 ccgttcatat ttatgggaat gattatcgaa ttgtctcggg cgcgcttcgt aatctgcggg
     7021 ccagcaaaat cacaatgaca aaggccagtg acatcaccac gatgccaggc aaaccaaatt
     7081 catcggcaaa acctgtattc ggcagcgctg tggacgtggg cggcacggtg ctcaacgccg
     7141 caagggcagc ctgtgtgtat gccgcgccaa tggtcgcggt ctgtgcaaca gaggtgccgc
     7201 tcaacccagc aagagcgggg tcaggtgtgg gggtggcaga tgcaacacca acgggagagg
     7261 tcggagtgac tgtaaaagtg gtcgtggctg tcggcaaaat ggacgcctcg aaagtggcgg
     7321 tgattgcgcg atttaccgtt tcattgaccg cattggcagt ggctgaagcc atggctgcac
     7381 ctgcaacttc tgtctcctgt acggcagcgc ggccctgtga accgaaaacg taaaccgctc
     7441 cgaggcaggc aatggagatc aggataatgc cgcctaaaat acccaccgcg atcaaaaacg
     7501 ttctattgtt atttgattct tcgggaagcg gggcatcgcc agcatcgtcg aaattgggtt
     7561 catcaaaaga catggaacac tctcctcgtg atatcacctt caacaggtga tttaattaca
     7621 actattttga atattatccc acaacttcaa gatttacgag aggtacgata acggtttcgc
     7681 cattttcaag ctttacttct gcagcatggg catgcagacc gctcggtaat ttggtcaacc
     7741 cggatttaat ggccgagata acaccgatcg cccccattgc aggcggacgg cgcatgcgta
     7801 cctgcaaacc tggcgaaaaa gtttcaactt cacgaggggc gggcgggtcg ctggagatgg
     7861 gcaggttaat gatgacttcc ggacgagcac cgctgtaacg gtcatacact tcagcattga
     7921 cggtgacatc acgtttggca ttggtactga gcaggcgata cgctgccgag ttcatcggca
     7981 tggggccaaa accatcggtc acaacgatcg ggtagcgcat ttcacgagcc aattgaataa
     8041 aggacgggta caaactggaa aggatcaacc cacgaacagg caattcggca gcggcttgca
     8101 gggtttcagc atccttgacc ataccggcaa gaatgatgga cccgcgcagg cttacatcca
     8161 gacgatcagc ggtcaacacg ctttcgggtt tgtctgcaag gttgacgagc aaccccgaat
     8221 caatgcgtcc attgccccaa acgccttgca caagtgcgcc tgcggtttga ataatcacac
     8281 cacggtttgg aacgatctcg atcacggtac cagcgatgcc tgcacgtaat tcgatcttgg
     8341 attcacccac ttccagcaaa acctgtccgc cgccgacagc cgccacacga ccttcacgcg
     8401 gggcgcggac gacccgtggg aacaacccct ccccaactgc cacctcggca tccgcaggga
     8461 caacatcctt gaaattgaat ttgatcaggc ggtctgccgc ttcgggcttt actcgcaatt
     8521 ggtgggcaac atttaataac acatgttcgc gtgaccattt cgcttcggcg atgacatcgg
     8581 acgggctgac cttttgattc aggcgtgcaa gcacagtgcc gttcacgggc aaaagccgtt
     8641 cgcgcacaat ggatgtcagg ccaacgatat ggtgtacggg cgcttgcatg agtctattcg
     8701 cctccgagag tccaattcca ttttttgatc aagtcacgac gacgcacact atcagcaggc
     8761 aaatcgagtg gacgtccacg gccatcgaag accacgccca atgcgccgcc actgaccggg
     8821 atcgtgccgc cacgtccggg gccgaaaccc acatcggtac cgcgcaggca ttgaaccgtt
     8881 aacttaccag tctggccatt tgccaacggc agggtttcaa gaccgccata tttcaaatcc
     8941 atgcgggctt cggtgccgtt ttcataggtc agtatggctc gcagaattgg ggtgccataa
     9001 gctgcggatg tcactggggc gaccaccgtc ccaacgctga ggaatgcacc cgattcaagc
     9061 acttgaacag ggagaatatt gttctgagat gcggtcacac ccaacagggg cagcaggtta
     9121 ttttggtcta tgatgacggt ggtcacaccc acaggttgga tcccgtcaag gagcagaagc
     9181 aggctctggc cggggcgcga agcgtcgctc aaaattccac cgcccgccag aatcggctca
     9241 aagagcggag tcagattcgg cttgatacca gccacattcc gtggaaaatc acggcgagcc
     9301 gattgcattg caaggaaaag agcctgccgt gcaatggcct gcgacatatc aaggtcttcc
     9361 ttggtggcgg caatggtgga tggatataaa gccttttgat acatgtaatc gcgcaacacg
     9421 cccggcgaga catcgagcga agaccagcgc aaaatgtctt cgagttttgt gtaattaagc
     9481 aaacctgtga ggttctcgcc aaggccgaat tgtggataca cattcaatga agatttgtca
     9541 ctgaaacctg ctgcaatgat cgcagccgag gcgccaaggt ccacgctcaa aattcccttt
     9601 tgagaactgt acaccttgga aaggaagcga accatgcgac cagtggcata ggcggtcggc
     9661 agcaggtgac cgcccgacca catatccaaa aggtccacgc ctctgatctg gcgtttgcgg
     9721 atgttgatgg tcatgcgggc cagttcacgt tgggcgggtt cgaggtcttc ggtatcaaga
     9781 gaaggacgca cattggggct gaagtgcagg gaggttgcca gtgaaccgat cagggttttg
     9841 atctctgctt cgatcttttc attgcccgcg tataacacag cagggcgttt ctcttcgggc
     9901 atggtgaaac tcgccaggcc gatcggctcc agcaattttt gaatggaacg ggatgcacct
     9961 ccatcggttc cgcctgcgat cacgatcagg tcaggtcttg cccgcagcac cgcgtcgatc
    10021 tgttgatcgg gtttacggcg gtcactgata ttcagggttt caacgatccg catgtaagtg
    10081 gattccgcaa gacgacgcgc gctttcaagc gatacatcct tgagcaagcc aacgaggact
    10141 gttttgatgg tgggtccagc cgacaaggtt gcaaccaacg catccacacc cgacccgttg
    10201 ggctggctcg gtgtaatcag gttacgtgaa ccatccagca gggagcgtcc taaaaccgat
    10261 tgcagatttt gaatggcatt gcggacacct tcgctcacat ctttgtacgg cgcttcagca
    10321 gtagagggcg caacgccaga agccacaaaa cggtattccc cttcgacaac atcgaagaag
    10381 accgctcgtg tattggccgc gccgacatca atggcgagta tggaattacc gtcgatcagg
    10441 gaggaagcca taattattgg aagttcccaa tgaaactgtt aataaaattg atgatcgagt
    10501 caatgcgttc gatcagggcg gttaaggctg ccgagtacac acccgcaaag accacaccca
    10561 gggtgatgcc aatgaacaag cggccgatcc atgcagggat ttcgatcaag gcgaagcggt
    10621 gcatggtgcc gtccgctttg gggcgtgctc caaagtgaaa gtagatcagg gtgaagatcg
    10681 tacccgccag gatggttccc ccatttacca atgcctcgat aaacccgacg ttacgtgctc
    10741 ctgctgcaac catatcgaag gaattgatcg caccagaaac ctgagggatt aatgtacctg
    10801 aaagagcacc cgcaatcgcc accgcagccc caacaccaac gatgaaagcc atcacaaagc
    10861 gagcaagccc ggccaatcgc ggcgaaatct tcatcaaaat taatgcaaac cccagcaggg
    10921 gaaagacaag gtatccgata ctttcaaaag gagtggatgg accaacgacc aaaggataaa
    10981 ggagcttcgg ccaaatcacc tgccaaatgg ctacagaagc aatatacccg gctgagattc
    11041 ccacgaaaat atagaccgcc acacgaaaaa gcggattgtc tccaagcaca taactaaaga
    11101 tcaaaattgt aaataacagg ctaagcgccc cggtgatcat atccagcatc tgaggtgtga
    11161 gaaccatgct acttcacctc cctggccgca gcgcggtcgc gcagacccaa gaccaaattc
    11221 cacaacccac ccagcaggat cagaaccatg gctagcagca ggccaatgtt gtaggcatcc
    11281 caataaatgc gtgtggtgcc cgggcggttg aggttattct gctcgaacac agcaccgtca
    11341 tacaaacctg ccaccaaccc gctgatctgc tgcgagtcga aatacgggcg gatcatcggt
    11401 gcagcctgcg cacttgagat cacaacaatg ggcattgcac cacgcgtaga acttgtctgt
    11461 tcgatccatg cgcgggcaga atcggcgtta tctgtcacaa tgacaaaagc cgtgaattgt
    11521 gaaagggttg taattccact ccatggttcg gctgcccagg cagggcttag ataggcattg
    11581 agcaagggac tttcagttac accgctgttg aacaaatagg gagcgctttg gcgcggattt
    11641 tgagcaaagg aataaatgcc catttgtcca ccgggcaggt aacccaaatt catatattcg
    11701 attccacttt ggtattggtg cccggccaaa ggtccggtag aaatgaagcg ttccgccaaa
    11761 atcgcacccg attcactcgt ggaaacaaat gtaaagcgcg ggtgatgtaa aagcaacatc
    11821 tggtcaaaca tcggtgcagc cacggcttcc atctcaccca cacgagcagg ctcgacatca
    11881 aaggccacca acacaggcgc accctctgga agcagttgag ccacatccaa agcgcctttc
    11941 atcgcaggct ttaattcact gggtggcagg gcgaagaatt gcgtccgcat aattagaatg
    12001 gcgggcagga tcaggaagaa caccgccaca aggaaccagc gcaatgcgcg ggatgtattc
    12061 aaattggaaa gcgaagaaag cggaactgga gcggtttcag cggcgaggat ctgctccaac
    12121 aaagccgcgt gtgattgctg ttcttcgttc gcttgcaaac ggattgaata agccttgggc
    12181 ttgctcgccg gagaaaagcc cggcacagca ggcaacacac cctgcaaacc agccaacgcc
    12241 ccccgcgact caagtgtttg gtcgctggaa agcgaaacag gcatggatgt cggcaaaccc
    12301 gagtccactg gccgcatggc ttgcacccag gaaggcaaat caccaggagc gatcgcatca
    12361 gtattggtga tcggtgatgg tgaggaagat actggtgcag ccatgtcatc ggctttcgaa
    12421 agccaatcag gaaggtcggt aaagaggttg tcaatccctg cctcggcggg aggagtggag
    12481 gaaaattcaa tctcagcagg tttcgatgaa gattcaggcg cagccgattg aagcgagttg
    12541 agccagtctg gtacagcagc agggtcggca gcgggtgtct caccaaagat ggtttctttc
    12601 ggagcagaag ccttcaacca atcaggcaca tcacccaacg aaatatcctg cgcttccgaa
    12661 ggctgtgcag cagattccgc tggattcaac catgcgggca tttccagttc atcaccggaa
    12721 gtcacacctg cactcgccca atcaggggca ttgtcaacag gtgtattttc agattcgctc
    12781 ttcaaccaat caggttcgga caaaggcgct gcggtggatt tttcttccgc tgtgcctttt
    12841 aaccaatctg ggacatttcc gccaaaaaga tcagcggacg attcagggaa tccactggaa
    12901 actggttctg aagaagcggc aggttctgcg ctttgagctt gcataccgcg taaccagtcc
    12961 ggtgtatcgt cggttgttgc cgagccgaat aagtcattgg aactgctgac tggnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaatccgg tgtgtcatcg gcggaagatg
    13141 caccgaacaa atcattggaa ctgctggcag gctggctgtt attctttgct tcattatccg
    13201 cagccatggc acgcaaccaa tcaggcgtat catcagctga agcggaaata ttcccaaacg
    13261 tatctgcagg ttcgctggat gaggtaaagc caccctgacc atcttctgca gccatggaac
    13321 gcagccaatc gggagagtcc acacttgaag atggagcggc ttcggcgggt ttttgaattc
    13381 cgcttgcatc acggaaccaa tcagtcaatt catctttttc ggtttgtggt gcgtttgagg
    13441 ccagaccagc caaccagggt ggggtatcag attctgcggc aggctgttca tcctgtacaa
    13501 aatcattctt ctcacccatc tccacccaac gcacttcaga aggctcaggc tgtgactttt
    13561 tcgatttcgg tgcatcaccg gttatgttgg caagccagtc tgggatatct tcttcatcgt
    13621 tgtcgtctgc ctgagaatgc agaccagcta aaaagtccac aggagatgcg ggttgggttt
    13681 gtttgggagt cgaaggttca gcaggtacat caccgctacc aatattccgg gccgagtcgc
    13741 gggcatcctt caaccattgc ggcagaatcg gttcaaggtc ggctgtattc ttttttgtgg
    13801 gggcctgccc gggcttcaag gtttcatcgc ctttggatga gccaggcaca gggccaagcc
    13861 gggtctggca attcaaacag aattcctgac cgaggcggtt gttctcacca caaacggggc
    13921 acttgatcat gtctgccatt aattgccccc gtatgggcgg tcaatgccaa agagaacacg
    13981 cagaccggtc ataaggatac ccagcgaaac accgatcaaa ataccgcgcg caccacccaa
    14041 agccagcact tgtgtcaccc actgtgaagc ctcaccaatg aggggaattc ctccaaaggt
    14101 tagggtggca gtgcccagca ggacaaatgc agctgtaatt aaaaagataa tactcatcag
    14161 atccacgcgg cggcgcaaaa gacgaatggc tgcataaagc agggaaatgg tgagtaatcc
    14221 catcaatgcg gcttcagagg gaataatgat gccatttacc accattttca tgatggtgtg
    14281 cccaggccgc agaaaaattc caaagataag ggtggccagt aggctcacca ccagtaaggc
    14341 actgtagacg ctgccctttt cacctcggcg cactttgtcg gaatgcacaa gggcaaggtt
    14401 gaaaacgccc acaatgacag ccacgccagc aaggatgaca gcccagttca ataacaatac
    14461 ttgtatgaca gagaaagccg ggatgaaata gcccagcaaa accaatacac cggcggtaat
    14521 ggcaataaag gcgaaaatta ctgagaagat tcgcatcaga tcatccccgc aaatttggcg
    14581 aacgcgccac ccatcagagc tagaatgatg aaccagcgca aaatatcttg cacggtcagg
    14641 ctggctgcat gggaaacgcc agcgccaaga taagcacctg cggcaaacag ttcttcgccg
    14701 atcaatgcgt cttgtgtatt ggcaaaaatg acggcctgcc cggtcagatc atcactggcg
    14761 ccgatcacgg tgatgttctc acggtcagca gtttcagcaa gtaacgcaac ttccgggcca
    14821 aaatgtccaa gcaaaatgtt ggttgaaacg ttttcgtgtt gggaaatgtt catcgcccca
    14881 gcagcgtaac caaatggggt caaacccgtt acacgcccag tggtggggac atacaactcc
    14941 tcaacacctg cagcgtgata tcccgcctgt aaagtatcct gcgccagcaa accgagcgca
    15001 ggatcgccag cagaggcaat tgacggctca tcactgaccg aggtccgctc agcgatatgc
    15061 cgcaacacag ccaaccctgc caaaccagag ccgccacgca catccagaaa actgccacgc
    15121 ccaagtgaaa tatgcagacg tgtaccgtct tcaatgctta aacccagtgt acgagccagc
    15181 cgtgttaagg ctgggatgtc acgtaaattt gcgggagatc tgcgtttcca aaaggtgact
    15241 gccagtaaaa ggagcgcact aaccgcaagg atggccaact cgatgatctt cacaacgaag
    15301 ccctctcttc aaccagatac gatgcaaacg cctgaccatc ctcttcaagg gtggcagcca
    15361 gggcagttag ttgatcgctc tcaaataaac cacggctaaa ataaagcgcc tgcgctccaa
    15421 gcaaggcaag aggacttcct gcttcaagga gcgaggcggc aaattcggta agctggtaac
    15481 ggcgcagggt ttctgcccag cgcggccagt attcacgcga tggcttcata taaagcgagt
    15541 atagcataaa tttaatgacg tctcgcgcag gtgttgatga caagtaaatt gcagactgaa
    15601 tgaacgaacc ctttttactc aggcccgatc ttcgtccctg attcgatcac catatccttg
    15661 ggaatcacca caattccatc gcggacgtac cactttgcat tgtcaatatc cgtgttgcgc
    15721 gggaatggac gaatgacaac atcactgccg atacgtacgt tcttatccag gatcgcgcct
    15781 tcaatgtggc aattttcgcc gattccaatg ggcaaccctt cgtcatcgcg ttcatagtaa
    15841 tcagaaccaa gcatgatggt gtctttgatg atgcatccac gcgagatctg gctgcggata
    15901 ccgatgatgg aatgggtgat atccgatttc tgaatacggc tgccttctgc gatcaacaca
    15961 tcacggatct tgctatcctc tacaatggaa ccggggagga agcgtgtgtc ggtgtagatg
    16021 ggaagtttcg gatcataaaa attgaacggg gtttctgaag tggtcagggc caggttggtt
    16081 tcatagaaag aacggatggt tccaatatcc tgccagtagc cgctgaaatc gaagccatag
    16141 accgagtagg actgaatcgc ctgtggaata atatcgccgc caaaatcgtg aaaactgggg
    16201 aagtttgaaa gcaattccat cagcacacgg gttttgaaca tgtaaatacc catcgaaccg
    16261 aggaaaggac gcaccgcatc atcacggctg atgaacttat tctggacttc gacatccttg
    16321 ggtttttcta cgaacgcgga aatgcgacca ttcttttcgc gcttcaaaat acccaaacgt
    16381 gatgcttcat cacgcggtac aggctgtact gccactgtaa tatctgcctt gctattccaa
    16441 tgatattcag ccatttcatt gtagttcata cgatacaggt gatccccggc aaggatcagc
    16501 acattcttcg ccccactgga ttgaatttca agagtctgtt tacgaaccgc atccgcagtg
    16561 ccctgatacc agtcagcact ctcaatggtc tgctctgccg cccaaatctg tacccagcca
    16621 ggatggaaag agtcaaaatg gtaggtattc gtaatatggc gatgcaaaga taccgagttg
    16681 aactgggtca acactgcaat acgatagatc cccgagttaa tacaattgct gatcggaata
    16741 tcaatgaggc gatattttcc tgcaatcgga accgcaggct tggagcgcat ctgcgtcaaa
    16801 ggatacaagc gtgcgccgcg acctccaccc aaaatcactg caagtacttc attcaacgag
    16861 ggcatagtag tgtctcctga agcgtatttt actgtttttt tcgcaacttg ctaagttgga
    16921 ctttccctcg attcatggct gtagcaaagt caaaaaagct caatgtgaac gacgcgaata
    16981 aaactataga tcttgaccct aatgaatggt tttttttgtt tacccgtgaa taccagccag
    17041 cacttttaaa gatcacggca tgtgtttctg cctgccaccc agcctcttct taacatgatg
    17101 aacaacataa acgatcgggt aggccaggag gatcaacaaa tctatcaagg cgaagaaaag
    17161 ataaagtttc atcacacacc tccttcataa aatcaaacta cacagtacaa agcataacgt
    17221 aaatgctgta tataccattt tacagttaaa aaacagggtg tcaacgcagg cagactcgat
    17281 gcatataaaa ctcaaacgtc ctcccatgat gggaggacgt tcgggttata ataccgcttc
    17341 gggtatcagc cagtgccgaa ggagggaatc gaaccctcat tcccggaggg aacacgattt
    17401 tgagtcgtgc gcgtctgcct attccgccac ttcggcattc ggttactgag cgcccgatag
    17461 tataacaaac tctctcgaat aaggtcaaga tgaaactagg aattatcgga ctcccccaat
    17521 caggaaaaac caccattttc aacgcgctca cgcgcagcaa tacccccacc agcgcatccg
    17581 ccgggcgtat cgaagttcac accgccgtcg tggatgtacc cgacccgcgt gtgaccaaac
    17641 tctccggcat gttcaacccg aagaaaacca tctacgccaa agtgacctat gcggatattg
    17701 ccgggctgga atctggttcg gccaaaagcg gcatttccgg gcaactgttg aatcaactca
    17761 accaaatgga aggcctgatc ctcatcgtgc gcggattcgc ggacgataac gtcatgcacc
    17821 caaatggcag tattgacccg ctgcgcgatg tggacagcat gttaaccgaa ttgctgctca
    17881 acgacatgat cgccgtggaa agaaaacttg aaaaattgac cgaagaacga aaaaagggcg
    17941 gcaccgacaa agtcatcaat gcgcgccaga cagagttgtt cgaaaaactc cacgcaaccc
    18001 tttcagataa caaacccctg cgcagcatgg aatttacgaa cgaagagcaa aaagaactcg
    18061 ccagctatgg cctgctcacc cgcaaaccca tcctcaccgt gttcaatatg ggtgaaggac
    18121 aaaccgcgcc cacagcccag ctcgatcacc ccagtgtggc cttgatgggc aaactcgaaa
    18181 tggagatcgc ccaactcagc gcggaagatg ccgccgtttt tatggaagaa tatggcatca
    18241 aagaattaag cctcaaccgc atgatcaacc tctcctacga cctgttgaaa gtgcagacct
    18301 tcttcaccgt cggcgaagat gaagtgcgtg catggacaac acgccgtgga gcaagcgccg
    18361 cagaatcggc agcggaaatc cacaccgatc tgcagcgcgg atttgttcgg gcggaggtgg
    18421 tttcctacga cgacctgacc agtctcgggt cgatgaatga agcgaaggcc aaagggaagt
    18481 taagactcga aggcaaggaa tatccagtta aggatggtga cattatgcac atccgatcca
    18541 gtctataatc tagttgatac gcccttctgg tttttatata atgagattgc ggagattaag
    18601 ttccgcaatc tcattttttt gggagggcac catgtttaga gagcgtgggg tatggggggc
    18661 catactgcta tcgctgatct ttctgttcgt tctggcctgc ggcatatccg taacgcccaa
    18721 caccagcagc ccgcctgcat ttgacccaac caaggcggcg cttgaactcc aggctacaag
    18781 catgtctctg caattaaccc aggccgcatt gaatgcgcaa caagcggccc agtcaacatc
    18841 tcccccacca caaccaaccg cggcaccaac agaagcgcca gcacaagccc agcccacacc
    18901 cacacaggat gtggaagcgc gcatccgcgc cgccaatgtg ctggtttacg aaaacacaga
    18961 cgagtacggc atcggcatgt ggattcaaga tgcgcttgac aacatgggca tcaaatacac
    19021 gcagaccggt tcctacagcg ggcacttcat ggagtatctc aactccggca caaaatatga
    19081 cctgatcatc gtcggcgcgg aggcaaagga tcgcatctcc ggcgagtttt gggatgtgat
    19141 caacacccgc ctgacccgcg acaaggtggc actgatcgct gaggtctggt atctcgacac
    19201 agaagcgaat gggccgatca gcaaaatact gggcggctgt ggaattcgct accaaaagga
    19261 ttatcaactg gcggattcga tctactggtg ggactcaaca catcccgttt tcaatgaacc
    19321 gaatacggtg cttccgcttc ttcactatga ccgatactgg gccaaccaaa ccggagatca
    19381 gattcgcctc ggcggctccg gggatgccgt gctgctggca ggcttggctg cgcagaaatc
    19441 aaatgaggaa gccgtgctgg ccacctgcta tgaaggccgt gtcatcatcc agaccttctc
    19501 agaccatgat tacaaaatgg cagatgtgat tccgctctgg gagaattaca tccactacac
    19561 gttgaaaaat cactttttgg ctgtgccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19681 nnnnnnntca aaaatccatc ccgatgacgt ataatttgga ggctgtaaat tattttttgg
    19741 agaagacaaa atggcttata cccagaaaaa atggagcctt gctccactgt accccggtta
    19801 tgaaagcgcc gagctgcaaa acgcgtttga catgatcgaa gagcaggtca cctcttttga
    19861 gggcgtgcgc ggaaaactca accctgagat ttccgcggaa caattcatgc agatcgtccg
    19921 tgccagtgaa gagacgctgc gcatcgcaaa caagttatat tcctttgcgg gcctgtcttt
    19981 tacagcagat acgcaagacc aggcggcgca atccctgcaa aaccgcgcca tgcaatttct
    20041 ggcagagatc gaaaaccgca ccatgttctt caatttatgg tggaaggaag tggacgagac
    20101 caatgccagg cgcctgatgg atgccgctgg cgattaccgc tactatctcg aagcgctgag
    20161 attgttcaaa ccgcacaccc tgaccgaagc ggaagagaag atcgtcaaca tcaagaatgt
    20221 gactggctcc aacgcgctga tcaatctcta tgatgcgatc accaatcgct ataccttcaa
    20281 gatgcgtgtg ggcggcaaag aaaaggaaat gaccgcggca gagcttcaat ccttccgcta
    20341 tagcaccgac ccgaaagtgc gcgcggaaag ttatcaggag cagttccgcg tgtatggcaa
    20401 tgacggcgtg atcctcgggc aaatgtacca gacccgcgca cgtgactggc acaacgaaaa
    20461 cattgacatg cgcaagttca ccagtccgat cgcggcgcgc aacttgaaca atgacatccc
    20521 caacgaagcc gtggatgcgc tgttgaacac agcccgcaag aatgtaaaaa tcttccagcg
    20581 ttatttcaac ctgaaagcca aacatgtcgg catgaagaaa ctgcgccgct atgacattta
    20641 cgcgcccgtg gcacgctcaa agaagactta cgaattcgat accgccgcag acatggtatt
    20701 ggaggcattc tctgcattcg acccgaaagt ggcggaattg gccaaacgtg tctttgatga
    20761 agaccgcatt gacagcgaaa ttcgcaaggg caagcgcggc ggcgcgttct gttggtctgt
    20821 cctgccggag atgactccgt gggtgctggt caactatcag gggactggac gcgaggttgc
    20881 gactctggcg catgaactcg gccacgccat ccattccatg ctggcggcgg ggcacaatac
    20941 cttttcgttc cactcctctc tcccgttggc agagaccgca tccacatttg cggagatgat
    21001 gctgatcgac aagatgctgg ctgaagagac cgatgaatcc gttcggcgcg acatcctctt
    21061 caaacaaatg gacgatgcct acgccaccat tttgcgccag tcatattttg cgctgttcga
    21121 aaagcaggca catgaaatgg tgcagaagaa cgcctccgtg gatgagttgt gcgcagccta
    21181 catggaaaac ctgaaagagc aattcggcga ttcggtggag ttgagcgacg aattcaagtg
    21241 ggaatgggtc ggcattccgc acatttacca caccccgttc tacgtgtatg cctatgcctt
    21301 cggacaattg cttgtgcttt cgctgtatca gcagttcaaa gcggaaggcg aatcgttcaa
    21361 gccgaagtat ctgaaaattc tttcggcggg cggatcagaa gcgcccgaaa aaatcctcac
    21421 cgaagcaggc gtggacatcc gctcggcgaa gttctggcag ggcggtttcg atgtactcga
    21481 aaaactggtg agcgagttgg agcagttgcc agcggataag ccgaaggtga aaaaggctgt
//
LOCUS       DAOQJC010000064        20878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1156_length_20878_cov_9.258608, whole genome shotgun sequence.
ACCESSION   DAOQJC010000064 DAOQJC010000000
VERSION     DAOQJC010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 20878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20878
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1156_length_20878_cov_9.258608"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /gene="atpA"
                     /locus_tag="PLE39_11045"
     CDS             complement(<1..1235)
                     /gene="atpA"
                     /locus_tag="PLE39_11045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNE68910.1"
                     /translation="MSDLIKQITGDLQKQIEGFKPELNVSDIGVVVEAGDGIARVQGL
                     ANVRAQELVQFSNGVMGTAFNLEQDSVGVIVMGEYDEITEGMTVRGTGRIASVPVGDA
                     MIGRVVNALGEPIDGKGPIATTGFRPIERIAPGVVERQDVDTPVQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTAIAIDAIINQKGKGVVCIYVAVGQKKAAVARTLGILEKYGAM
                     EHTIIVMASADESAALQYIAPYAGTAIGEEFMETGRDALIIYDDLSKHAWAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERSARLANQFVVVKKDYSNDLADAKDGVDGKVYTGP
                     LSKHEAEEAAKAMGDGFKAVKVKGTGGSLTSLPIIETLLGDVSAYIPTNVISITDGQI
                     YLESNLFNAGIRPAVNVGIS"
     gene            1508..2647
                     /locus_tag="PLE39_11050"
     CDS             1508..2647
                     /locus_tag="PLE39_11050"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68911.1"
                     /translation="MSEINREEGKPPSRRMKRYWKVAVLANIKDDNQPKPEGVPPDAF
                     ADFDHIETIDSLRAAIETDGHTTYFIQADRDLPFALREIQPDICFNIAEGLGGDAREA
                     QVPALLEMLGIPYTGSRVLTNGISLDKTLTKRIWRDRRLPVAPFQEFNAGDEPLRPEL
                     KFPLFVKPAREGTGMGVDLKAIVNNEKELRERAQYIVNVYQQPALVETFLPGREFTVG
                     ILGRADSKLYSRHPEWYEKDGFHRFPVLELDSTRSVTPWVYGLEAKSKDVGADGSPGY
                     FCPADIEPELEKKLKNFALRAHQLLNTLDVSRTDIRLDEDGNPRVMEINTLPGLTPDY
                     SDLCLQAKAEGIRYEDLVLDILYLGASRWGMLEPREYVPDTPKKR"
     gene            complement(2690..4282)
                     /locus_tag="PLE39_11055"
     CDS             complement(2690..4282)
                     /locus_tag="PLE39_11055"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNE68912.1"
                     /translation="MQTNNRLGCLTGTGIFAALVTIAAIAGLAFFSGSQMFTAGALNA
                     EPGDEVLGSVNSHAQITECSACHAEPWSATRMADHCAKCHTDIAAQMYDVAQLHGAIM
                     QKNNTLACRDCHPEHRGASASLTEMGENTFPHEALGYSLEGHQQKATGEAFICKDCHG
                     EDVTTFASNSCDNCHRQMDIVFTQTHVLSFGTDCLACHDGIDRYGDNFTHDQFPFQLK
                     GEHAEANCTECHLDARSIADLQSLPQDCFSCHREDDPHGGAYGNECGVCHAPEGWLPA
                     KFDHNLSAFKLEGEHNEVACEKCHVNNVYKGTPSDCYSCHQQDDEHNGRFGTQCETCH
                     TPKNWESANFDHNLTAFKLDGEHREVRCERCHVNNVFKGTPTDCYSCHQQNDEHNGKF
                     GTDCASCHNTNDWDDATFDHSRTNFPLTGGHSGVDCEQCHKGDSFTGLSNECVSCHAD
                     PAFHAGVFGTNCATCHTIFAWTPAGFNIQHPEPIAEEGGNGVFHGGASCRQCHPSTVQ
                     EATCTACHDGNDFEGGEGGDDD"
     gene            complement(4292..4846)
                     /locus_tag="PLE39_11060"
     CDS             complement(4292..4846)
                     /locus_tag="PLE39_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68913.1"
                     /translation="MLRGNKELWLAFVFCLLITGLYGAVVIWTREIPAAGELFGHSIG
                     IVGFVLMLMTEVLYSLRKRSRSAALGRMSTWLQFHIFTGLVGPFMVLLHTSWKFNGLA
                     GATTLLTVIIVFSGFIGRYIYTRIPRTLDGLEIEGTLSQEALKQARRLMSLWHTVHIP
                     IGMALFISAFIHMGAALYYATFLR"
     gene            complement(4897..6195)
                     /locus_tag="PLE39_11065"
     CDS             complement(4897..6195)
                     /locus_tag="PLE39_11065"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE68914.1"
                     /translation="MRYIIIGSGVAGVAAIEAIRKNGMSGEIVMVGDDPHGFYSRPGL
                     AYFLTGELHDKALFPRTREDYVRLHFKFHKSRVTKLLPSEKAIELDGKSHLSYDKLLI
                     SVGAQAIPLTVPGANLEGVVKLDHLEDARKILKFARRGKTAVVVGGGITALELAEGLL
                     SRGVKVHYLLRGDRYWSNVLDDFESRIIERRLQEEGVTLHFHTELAEITGRSRVNGIR
                     LKDGRAMRCDMVAYAIGIKPRIELARNAGISVDRGILTNEYLQTNHEDIFAAGDVAQV
                     FDPVTGRSVIDSLWHLARDQGHTAGLNMTGERKTYIRNTPFNVTRLAGLTTTIIGSVG
                     HGHDDDIVGIARGDSETWRQLPDAIVAQSGFDVNRVRLMVGETTLIGAVVMGDQTISA
                     PLQRIVRDRMDITSMRDKLLARDAKIADVIVDFWSKYKTN"
     gene            complement(6192..6458)
                     /locus_tag="PLE39_11070"
     CDS             complement(6192..6458)
                     /locus_tag="PLE39_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNE68915.1"
                     /translation="MSFINIFKTDSTKRVALSAGHVVPDSSRCVQCGICTYNCPIGID
                     VRAHAWEGQAIKHSECLTCGECVARCPRGVLRFERTDLFAEGGK"
     gene            complement(6633..6845)
                     /locus_tag="PLE39_11075"
     CDS             complement(6633..6845)
                     /locus_tag="PLE39_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNE68916.1"
                     /translation="MSERFVGTVKWFNSTKGYGFIGRDGGEDVFVHFTAIQSDGYRKL
                     EAEQKVEFSIEEGPKGLQAANVVPLS"
     gene            complement(6952..7620)
                     /locus_tag="PLE39_11080"
     CDS             complement(6952..7620)
                     /locus_tag="PLE39_11080"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNE68917.1"
                     /translation="MTLPKKLTLEFHCHTHASKDSLTHPKDLVQAARRKGLDHLVITD
                     HNSIAGARAAQALDPELVIVGEEIMTTKGEILAAFVQEEIPPFLTPQETLMLLKAQGA
                     FISVSHPFDELRAGGWQEKDLLDVIPFVDAIEVYNSRCMLPRFNRKALEFARLHGLAG
                     TVGSDAHAVFEVGRSVLLLDQFAGPAEMRKVIRSAEFRVKWSPWWFHFVSRYASIRRR
                     FLKS"
     gene            complement(7617..8765)
                     /locus_tag="PLE39_11085"
     CDS             complement(7617..8765)
                     /locus_tag="PLE39_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HNE68918.1"
                     /translation="MMTTETKSIRWLKYVSGWLNKFNKSSLSDPSNLLLERLRRECDR
                     KEMKKAWSIIEDLDRLRQASDGLEPAEICLQCGLSVAEMGNFREAQRLFSEASTKYLN
                     SFHHQAVALWMKGCMDWLLPNKEVEAINSWREAEKRFKAIRDKENQKSRLDWYDARCK
                     EMHDALHQAAEKYQIPPLPAETPENKSLFAEMATNNSREDAEQQVRSDRVGLFSIHES
                     INAGDFGPSGVLNTPIEGMVEVEQVFIEDKPYRIVSVDGHSFFRSSTHGTAVIKVSGD
                     SMNLANIGSGDYVLLRFLPKNPQVIAEPDESGDMPPLTSYRNGDIVAAEIINDEGDGV
                     TLKRIVVRGGRIVLQPQSSNPVHVEREFNISDPHLYICGVVQAIFKPL"
     gene            complement(8762..10219)
                     /locus_tag="PLE39_11090"
     CDS             complement(8762..10219)
                     /locus_tag="PLE39_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNE68919.1"
                     /translation="MMIFRSAKTRVSRILQNLLVGDLIWARLRGLFLLFATAAVSVFI
                     AAGFSADKLTLLRIWNDIAFPMIWLAASILLSAYILVERYEEERAKPLKQVWYVIVFA
                     IFIGPLSWFEFVYVRPLLTGEPISRFLILPLAALISAFLSGARYVQDVFNIKHYVLAV
                     LYLLSSFCGIKYPKIKISENYVVGDGKNRIDQIGGPGYVFIGPGNAILTERLHGPAGV
                     YSAGRFFLSRFETIVSTLNLDDQHGLIPAISAVTKDGIMVTVKDVQFRYRVWSGHREI
                     NTAAGRSLTNPYPFTVQAIRNLTYNRTVSKSGPNTWHDMVKGVISGAVSDYITKHQLD
                     QITAPRYTDGDARKEISAQLKTSSTRDKLKDAGAQLLWCDIGHFEVDNKAVSEQRIDT
                     WKAGWMGNADVKRAYGEAQRNAYQEIGHAEAQAEILMSIVHAFDDIDLAKEDKDRSIR
                     NIILMRTAQVLEALSVSEGSEQNTDKNAKKDGKKP"
     gene            complement(10216..12006)
                     /locus_tag="PLE39_11095"
     CDS             complement(10216..12006)
                     /locus_tag="PLE39_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68920.1"
                     /translation="MPTDTRISRQAKLSILGTAVLVISLSGLSFFIEIKLVWQLVISW
                     GYVYILRAADAVYVSPEASLASSMLFANISVFVLIFFVVLFWVSPFILPVRGLVQTQK
                     AFTGLIRSLAGKKWHGPAIFVEAGKPNMEEHELDSHKAGVALVDLYSAIVLEQQTGSI
                     DSLRQEVHFEAEEEDVQGLQRPGMWKQITAKLGVAGKDVNVTFVKAFGPGVVFTESGE
                     KIVGFVDLRKQSRSVQGVKACTRDGIEITTNVNVTFTLGQAEDILKVTRTDLGWRAVQ
                     ITAPSAEDLVDRPEPRPGDQVVKFQPVDTINEQDMNELEHAFTEKEFEWSDTDIRIVK
                     VGAASQFVFEKERVFSAVYSRARSATGESQLIDWTELPGYVAADIFREQLAHENYDDL
                     YKPEEPNEFPMGDFKRKFGRMVTNQGMLGYQVVMLADETPIKDAQFVNEADLKFSPPL
                     RFKGPAILRDRGIKVLSAGFGDLVPVNEDVRAQLFENWRAHWQQEAQKTLANHELRAM
                     RIHNHERSRTQQDMIYSLSRIFHENQYTEEALALRLYQALEAAAINPATQRLLPADTV
                     QMLSNLRHWLLPENKKSDESNPVIGEEGTP"
     gene            complement(12012..12467)
                     /locus_tag="PLE39_11100"
     CDS             complement(12012..12467)
                     /locus_tag="PLE39_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68921.1"
                     /translation="MENIGGLAAEIVVAFVIALATGAFTFMGFWRQAKADLEQEYLKR
                     FNDKKWGVYTEFAKLLQAPSLNTFETDHRQEAETAIASQIVLIGSDEVVRAFRDWRES
                     VAVHGKAQRVTNEKLFALVANMRRDLGIKYSNLEVDDLLGVLNPSMGDG"
     gene            12706..14466
                     /locus_tag="PLE39_11105"
     CDS             12706..14466
                     /locus_tag="PLE39_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNE68922.1"
                     /translation="MKTRNFLIRIGLALLVAALLGAGAGLLAREKVDAAEGTYKILAW
                     NDLGMHCYNRDFADLAVLPPYNTLWVQVIKAGDPPTLVTSGVTVEYFYEDNTYSVGKT
                     NFWSYSKDLFGVNLPPNVGLKGKGLSGTMDFSGDHFVAEGIPVTEFSDSAPTTSQPYQ
                     LATVIVKDSATGAELARTRVVTPVSSEMRCDKCHSDGGEASPDNPTGKVETNILQLHD
                     EEEGTNLMANRPVLCADCHASNALGAPGQSGIPNLSNAMHNKHEDESPNTLNGCYNCH
                     PGPETKCLRDTMSQEGMTCIDCHGGMSDVKNNPDPWLNEPRCDTCHDSGQYDQNHPLY
                     RFSTEHGGLYCEACHDSTHAVAPSREENDAIKFNILQGKNGPVDQCTVCHTTTPSTGS
                     GPHGLTSSGALFEDVPSTYWARDFIERLYNAGITSGCSTSPLMYCPDATVTRAQMAIF
                     ILRGMHGSAYIPPTASGTVFSDVPAGSFAADWIEQLAAEGVTAGCGGGNYCPDATITR
                     AQMAIFLLRGEHGNAYTPPAATGTVFGDVPLGSFADAWIEQLAAEGVTSGCGGGNYCP
                     DNSVTRAEMAVFLVRAFSLP"
     gene            14646..16106
                     /locus_tag="PLE39_11110"
     CDS             14646..16106
                     /locus_tag="PLE39_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNE68923.1"
                     /translation="MNTKRLFSRIIAILIVVIFFLSGAAPTLAAPAPIPLYADDPTPP
                     AQTVKLIFIHHSTGENWLADGYGNLGIALEQNNYFVSGTNYGWGPNAIGDRTDIPNWT
                     EWFRSGDTETYMNALFNESGQNSSFTRNLTDPGGENEIILFKSCFPNSALEGSPTDPP
                     SPDGWLTVGHAKYVYNEILQYFASRPDKLFIVITAPPLQDGTYAENARAFNEWLMNDW
                     LTENGYTLPNVAIFDFYNVLTGPDNHHRYINGTIEHVFTSGMNTSYYPSSADDDHPSA
                     AGSQKATDEFISLINIFYNRWRNSTSSTFTDVPSTYWAAAYIERLYNAGITSGCSTSP
                     MMYCPLAPVTRAQMSIFILRGMHGSAYIPPAATGTVFSDVPADAFAAAWIEQFASEGI
                     TSGCGSGNYCPDANVTRAQMAIFLLKGEHGSSYVPPTATGIFTDVPIGSFAADWIEQL
                     ANEGITSGCGGGNYCPDADVTRDQMAVFLVRAFSLP"
     gene            16324..17628
                     /locus_tag="PLE39_11115"
     CDS             16324..17628
                     /locus_tag="PLE39_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNE68924.1"
                     /translation="MSISNATPVLALDEKNLIPVSDHLKQMADIPPSRMFLINKSLKV
                     YVEKNAGAKIFDASQGDGGASLPGTPKAILERALQLQLEHGTAYDMPFGTEAYRKAVI
                     EQYWKLDSSSGWGPANVLGTAGGRDALVKVYGAMLALGHGRQGDLIMVSRVPWISYNW
                     GPYGVGANVLWAPGDPAQGWAYSEDAIRESVKFAESKGRKIAGIVITNPDNPTGLTIA
                     VEKQVSLAKAALEAGVAFVLFDWMYHYVTDESPMDLNSFLKNFTPEERKRLMFLDGIT
                     KSLGGSNIRNCHLIADEAVIKFIMARASHGVIPSFYSLAVAMAAYEMGFTEAAKTIIE
                     PTNASRAVLKKLLAESGMQHIIGKGYYAFMNVGEFIKAKGWADSEPLGQYLAENHGVA
                     IVPGAFFSPFGGDWIRFSYATPTERTEGAFKRLVEGLESLKK"
     gene            17723..20518
                     /locus_tag="PLE39_11120"
     CDS             17723..20518
                     /locus_tag="PLE39_11120"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNE68925.1"
                     /translation="MPQQFADKYLLAVEQAIKEKPQGGLDGFEQEWNLLDEELRPLLT
                     VGAGPSQHSFVDYLRAECVPHWHQQFSQLEVFHWMVEWATRPYYTPRGAMYEARLMEA
                     TLMNALHRAGVNFGERLHYWHGNLLFLTDIGHQSIPGNWSLAKRRYLEKCVDLYGDTL
                     ATTGIHTNMSLPDPLFAWDFMHLSPSERGDQHLDEFKSEFYITATRLLRAFASLFIAT
                     SASTPMQAQVKDGRAAVILTEFDSIRNLTFPNPREIDLPDLYRSYNDYLQISYDLVRR
                     GVRFGNNNWTPVRARSFSEPVERIISTTSDQLSSLYARGLFAAGQSTPPEEMARQVER
                     QNLMARINLPMGRVEVRVDEGGHSLDLDIANMTFRHLLLLKIYSDPQFARGFRYDRED
                     ITRARANENMAAKHGLRAEIENPLTGKPITVREFLKWTLNELKPLAQGLNMWDDLHPL
                     VEMSEGGRNTSEKIRARLKMEIGDDNEVPLPLLKEFFYEHEAQVKADVETICANHASL
                     GADSAKIAEFIQRSRDTVRSSEIPNAPVQFRPRAQAVIEVSYPNKTAEILDLAQQLIR
                     IPSVTACPVERFEEVHRAGSLIDDYLRNAGLDVKYFDGKYPAIYATFPGASKPNPILL
                     TGHFDVVEPEPDDSQFNPRIEGDYLFGRGAADMKTVVATYLVWMKDMCKSASRPNIAL
                     MLVGNEENGEAEAWGTPHVLKELNLTPSLFIAGERTGEKGNELFGEICVENRGVMRFD
                     VIARGAKGHSGVAGTGDLSEKLIAARSALNEIFASHLTLKAADGWQSQAKFPFITVGT
                     PGVYNVTAAEGILGVEIRPIPQDDVTGLRLKVESYCAENGLEASFNVMENGVACDPNN
                     PALKALIEAVRQASGGEEPLIGKKLPGTSARFAPGGQAVVWGQSGVGPHAKNEAHYIP
                     SIEPYYNSLNELAKLWK"
     gene            complement(20587..>20878)
                     /locus_tag="PLE39_11125"
     CDS             complement(20587..>20878)
                     /locus_tag="PLE39_11125"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE68926.1"
                     /translation="KPLTQVDLANHAKMDVMMTSNVLRTLEEKGLILRKPHPTDTRAK
                     ALSITAQGRKLAGQAVPAVEAIDRKFFSQLGADARRFNEQLLKLIVANYADG"
BASE COUNT         5349 a         5853 c         4852 g         4824 t
ORIGIN      
        1 gagataccca cgttcaccgc aggacgaata ccagcgttga aaaggttgct ttcaaggtag
       61 atctggccat cagtaatcga aataacgttt gtcggaatgt aagcagaaac gtcaccaagc
      121 agagtttcaa taatgggcag ggatgtgagt gaaccaccag ttcccttcac cttgacggct
      181 ttgaaaccat ctcccatcgc cttggcagct tcttcggcct cgtgctttga aagtggcccc
      241 gtgtatactt tgccatccac accatctttg gcatcagcga gatcgttgct gtaatccttc
      301 ttcacaacca caaactgatt ggcaagacga gcggaacgtt ccaacaggcg ggaatggaga
      361 taaaacacat cgccagggta cgcttcacgt ccgggggggc ggcggagcaa caaagaaact
      421 tggcgatacg cccaagcgtg cttggaaaga tcatcgtaaa tgatgagggc gtcacggcct
      481 gtttccataa attcttcacc gatggcggta ccagcatatg gggcaatgta ttgaagcgcg
      541 gctgattcat cagcagaagc catcacaatg atggtgtgtt ccatcgcgcc atatttttcg
      601 agaataccga gagtacgggc aacagcagcc ttcttctgac caactgctac ataaatacaa
      661 accacacctt tgcccttttg attgatgatc gcatcgatcg cgatcgcggt cttaccggtc
      721 tgacggtcgc caatgatcaa ttcgcgctga ccgcggccga tcgggatcat cgagtcgatg
      781 gacttgatac cggtctgcac cggggtatcc acatcctgac gttccaccac gcccggcgca
      841 atgcgttcga tcgggcggaa acccgttgtg gcgatcggac ccttgccgtc gatcggttca
      901 ccaagagcgt tgaccacacg accaatcatt gcatcgccca caggaactga agcaatgcgt
      961 ccagtgccgc gtacggtcat gccttcggtg atttcatcgt actcacccat cacgatcacg
     1021 ccaacactat cctgttccag gttgaacgcc gtccccatta cgccgttcga aaactgtaca
     1081 agttcctgcg ctcttacatt tgcaagaccc tgcacacggg caataccatc acccgcttca
     1141 actacaacgc cgatgtcact gacgttcagt tcaggcttaa agccttcaat ttgtttttgc
     1201 aaatcgcctg tgatttgctt gatcaagtct gacattaggt agcctccacc aagattctta
     1261 ttaggacaag aacccggata agggcgttat ccgggtatat gtgtaatttt tgcccgtttc
     1321 cccgccccac gtcctttcgt gagtaagagt acaagcgggg caatgatacc tgaaagggga
     1381 atgattgtct agttgcaatc ccctcttacc ttaagaaatg tctcataccc ttgtcatcta
     1441 cacagcttac gctattattg cccgaaataa tcagaataaa cacatcttca gaaaacaagg
     1501 agacaagatg tctgaaatca atcgtgaaga aggaaaacct cccagtcgcc gtatgaaacg
     1561 ctactggaaa gtcgccgttc tggcaaacat taaagatgac aatcaaccca aacccgaagg
     1621 ggtaccgccg gacgccttcg ccgatttcga ccatattgag accatagact cgcttcgcgc
     1681 tgcaattgaa actgatggcc acacaaccta ctttatccag gcagatagag atctaccctt
     1741 cgcgctacgc gaaattcaac ccgatatttg cttcaacatt gcagaaggtc ttggcggcga
     1801 tgcgcgcgaa gctcaggtgc ccgccctgct cgaaatgctc ggaatccctt acacaggctc
     1861 ccgcgtactc accaacggaa tctcactgga caaaactctc acaaaacgca tttggcgtga
     1921 ccgccgactt ccagttgctc catttcagga atttaatgca ggcgacgaac ctctgcgccc
     1981 ggagttgaaa tttccactct ttgtcaagcc agctcgtgaa ggcacaggca tgggggttga
     2041 cctgaaagcc atcgtcaata acgaaaagga actgcgcgag cgtgcccaat atatcgtcaa
     2101 tgtatatcaa caacccgctc ttgtggaaac ctttttgcca ggccgcgaat tcactgttgg
     2161 gatccttggc cgcgccgatt caaaactgta ttcccgacat cccgaatggt atgaaaaaga
     2221 cggcttccat cgttttcccg tccttgagtt agacagtacc cgctcggtaa caccctgggt
     2281 ttacggactt gaagccaaat caaaagatgt aggtgcagat ggatctcccg gttacttctg
     2341 ccccgccgat attgaaccag agctcgaaaa gaaattgaaa aattttgccc tgcgtgccca
     2401 tcaacttcta aacacactcg atgtttcccg caccgatata cgccttgacg aagacggcaa
     2461 cccgcgcgtc atggaaatca atacactgcc tggcctgacc cctgattaca gcgatttgtg
     2521 cctgcaagcc aaggcagaag gcattcgcta tgaagacctt gtgctcgata ttctctatct
     2581 cggcgcaagc cgctggggaa tgttggagcc gcgtgaatat gtgccagaca ctccaaagaa
     2641 aagatagaat cacaaaaaaa gccctgcaaa atttgcaggg ctttttacat taatcatcgt
     2701 cgccgccctc gccgccttca aaatcatttc catcgtgaca ggctgtacat gtggcctcct
     2761 gcacagtgga aggatgacat tgacgacagg aagcgccgcc atggaataca ccattcccgc
     2821 cttcttcagc aattggctcg gggtgttgaa tattaaaccc ggcaggggtc catgcaaaga
     2881 tggtatggca agtggcacaa ttggttccaa atacgcctgc atggaaggct gggtcggcat
     2941 ggcatgatac acattcattc gataaacctg tgaatgagtc gcctttatgg cattgttcgc
     3001 aatccacgcc tgaatgcccg cccgtcaacg ggaagttggt gcgactgtgg tcaaaggtcg
     3061 cgtcgtccca gtcatttgtg ttatggcagg atgcgcaatc tgtgccgaac ttaccgttgt
     3121 gttcgtcatt ctgttgatgg caggaatagc aatcagtcgg cgtacccttg aagacgttgt
     3181 tcacatggca gcgctcacaa cgtacttccc tgtgttcgcc atccagtttg aaagcggtca
     3241 ggttatggtc aaagtttgcg ctctcccaat tcttcggtgt atgacaggtt tcgcattggg
     3301 ttccaaaacg gccattgtgc tcatcatcct gttgatgaca ggaataacaa tcgctgggcg
     3361 tgcctttgta cacgttgttc acatggcact tttcgcaggc tacctcattg tgttcacctt
     3421 ccaacttgaa cgcagaaagg ttgtggtcaa attttgcggg caaccagcct tcgggggcat
     3481 ggcaaacacc acactcatta ccatatgcac caccatgtgg atcgtcttcg cgatgacaag
     3541 agaagcaatc ctgcggcagg gattgtagat ccgcgatgct gcgggcatcg agatggcact
     3601 ctgtacaatt agcctcggca tgctcgcctt ttaattgaaa tggaaattga tcgtgggtga
     3661 aattatcacc atatcggtca atgccatcat ggcacgccag acaatccgtc ccgaaggaca
     3721 ggacatgcgt ctgggtgaag acaatatcca tttgacggtg gcagttgtcg caggagttgg
     3781 aagcgaaggt cgtcacatcc tcaccgtggc aatctttaca gataaacgct tcgcccgtcg
     3841 ccttttgctg gtgcccttcc aaagaatatc ccagcgcctc gtgcgggaag gtgttctccc
     3901 ccatctcggt cagtgaagct gacgcgccac gatgttcagg gtgacaatca cgacaggcca
     3961 gcgtattatt tttttgcatg atcgcgccgt gcaactgtgc cacgtcgtac atctgagcgg
     4021 caatatcggt atggcatttg gcgcagtgat ctgccattct tgttgcgctc cacggttctg
     4081 catggcaggc cgaacattcc gtgatctgag catgtgaatt gacactaccc aacacctcat
     4141 ctcctggctc tgcatttaat gcgccagcag tgaacatttg gctgccgcta aaaaacgcaa
     4201 gccctgctat ggcagcaatg gtgacaagtg cagcaaagat tccagttcct gttaaacagc
     4261 caagacggtt attggtttgc actcttccct cctatcgtag gaacgtggcg taatagagcg
     4321 ccgcgcccat gtgaataaaa gcggagatga acaatgccat accgatggga atatgaaccg
     4381 tatgccaaag tgacattaac cgccgcgcct gtttcaacgc ttcctgtgaa agcgttccct
     4441 caatttcaag cccatcaagc gtacgtggga tgcgggtgta aatgtaacgg ccaatgaatc
     4501 cgctaaaaac gatgatgacc gtaagcagcg tggtcgcacc cgcaagcccg ttgaatttcc
     4561 acgaggtgtg caacaaaacc atgaacggcc ccaccagacc agtgaaaatg tgaaattgca
     4621 gccaggtgga cattcgccca agtgctgcgc ttcgactgcg ttttcgcagg ctgtaaagca
     4681 cttctgtcat cagcatcaac acaaacccta cgatgccgat ggaatggccg aataattccc
     4741 ccgccgcggg gatctcccgc gtccaaatca caaccgcccc gtacaacccc gtaatcaaca
     4801 aacaaaacac aaacgccagc cagagttctt tgttacctcg aagcatcctc tgccctcccg
     4861 aaaaatgtat tcaactcaag ttgttgaggt tattatttaa ttcgttttgt attttgacca
     4921 aaaatccacg atcacatctg caattttggc atcgcgggcg agcagtttat cgcgcatgga
     4981 ggtaatatcc attctgtcgc gtacgatcct ttgcagcggc gccgaaatgg tctggtcacc
     5041 catcactact gcgccgatca aggtcgtttc tccaaccatc aagcggacac ggttcacatc
     5101 aaatccgctc tgtgccacga tggcatcggg caattgccgc caggtttcac tatcgccgcg
     5161 cgcaattccg acaatgtcat catcatggcc atgtcccacc gacccgatga tcgtggtggt
     5221 caagcctgca aggcgggtta cgttgaaggg agtattacga atataggtct ttctttctcc
     5281 agtcatattt aacccagccg tatggccctg atctcgtgca aggtgccata aactgtcgat
     5341 caccgacctg cctgtaacag ggtcaaagac ctgggccaca tcgccagcgg caaaaatatc
     5401 ctcgtgattc gtttgcagat attcattcgt caaaatgcct cgatctacgg aaatgccagc
     5461 attgcgggcc aactcaatac ggggcttgat gccgatggca tacgccacca tgtcacaacg
     5521 catcgcacgc ccatccttca agcggattcc gttcacacgg cttctccctg tgatttcagc
     5581 aagctcggtg tgaaaatgca gggttacacc ttcctcctgc aaacggcgtt caatgatgcg
     5641 cgattcaaaa tcatcgagca cattgctcca atagcgatct ccccgtaaaa gataatgcac
     5701 tttcacccca cgtgataaca acccctccgc caattcaagc gcagtaatgc cgccgcccac
     5761 caccacagcg gttttcccac gcctcgcaaa tttgagtatc ttgcgggcat cttccaaatg
     5821 gtcgagcttg accacacctt caaggttggc gccgggcact gtcaaaggga tggcctgtgc
     5881 cccgacagag atgagcagct tatcgtagga aaggtgcgat tttccatcca actcaatggc
     5941 tttttctgat ggcagtagtt ttgtcacccg cgacttatga aatttaaaat gcaaccgcac
     6001 atagtcttca cgggtacggg ggaacagggc tttatcgtgc aattctcccg tcaaaaagta
     6061 cgccagaccg gggcgtgaat aaaacccgtg cgggtcatcg cctaccatca caatctcacc
     6121 tgacattcca ttcttgcgga tcgcttcaat ggcggccacc cctgcgaccc ctgaaccgat
     6181 gatgatgtat ctcattttcc accctccgca aacagatcgg tccgctcgaa gcgcaacacc
     6241 ccacgtgggc aacgcgccac acactcccca caagtcaggc attcgctgtg tttgatcgcc
     6301 tgcccctccc atgcatgagc gcggacatca atgccaattg gacagttata cgtgcatatc
     6361 ccgcattgca cacagcggga tgaatcgggc acaacatgac ccgctgaaag cgccacgcgc
     6421 ttcgtcgagt ctgtcttgaa gatattgata aatgacaacc tgacctccat gtatcaagtc
     6481 gtttttcccc cagaaaagtt acggctttag tatggcaaat tctatcccct ccaccaatac
     6541 cccaccagtt acaaatgtca ttattaaata aaaacagggc tgcctcgcgg caaccctgcc
     6601 tgttcgcaaa acttgagccg tcaggcttag tcttacgaga gcgggactac attggcagcc
     6661 tgcaaacctt tggggccctc ttcgatggaa aactccacct tctgttcggc ctccaactta
     6721 cggtagccat cgctctgaat ggcggtgaaa tggacaaata catcctcgcc accatcccga
     6781 ccaataaagc cgtagccctt ggtcgaattg aaccacttga cggttccaac aaaacgctca
     6841 gacatctttc tatactcctg ctgagaaaaa aattgaagcc cgttcacctt tccgctttca
     6901 aaggcgaccc gggcctgctg agcgcaagat tatcatgcct aaaacagcat gtcaagattt
     6961 taagaacctc ctgcggattg aggcatatcg cgagacaaaa tggaaccacc agggcgacca
     7021 tttcacgcga aattctgcac tgcgaatcac cttccgcatc tccgctggac ctgcgaactg
     7081 gtccagcaaa aggacggatc ttcccacctc gaagactgca tgggcatccg aaccgaccgt
     7141 gccagccagg ccatgaaggc gggcgaattc cagcgctttg cgattgaaac gcggcaacat
     7201 gcaacgtgaa ttgtaaactt cgatggcatc cacaaacgga attacatcca acaggtcttt
     7261 ttcctgccag ccgcccgcgc gcaattcatc gaacgggtgc gacacgctga taaatgcccc
     7321 ctgcgccttt aaaagcatca aagtttcttg cggtgtcaga aaagggggaa tttcctcctg
     7381 tacaaaagcg gccagaatct cccctttggt cgtcatgatt tcctcgccca caataaccag
     7441 ttcagggtcc agtgcttgag ccgcgcgtgc gcctgcaatg gaattatggt cggtgatgac
     7501 cagatgatcc aatcccttgc gccgcgctgc ctgtaccagg tctttggggt gggtcagcga
     7561 atccttcgag gcatgggtat gacagtgaaa ttcaagggtc agttttttag gcaaagtcat
     7621 aaaggtttaa agatggcctg caccacgccg caaatataaa ggtgcggatc agaaatatta
     7681 aactcacgtt cgacgtgtac agggtttgaa ctttgtggct gcaggacaat tctaccgcca
     7741 cggaccacga tccgtttcaa agtgacgcca tctccctcgt cattgataat ctctgcagca
     7801 acaatatccc cgtttctata actcgtcaac ggaggcatgt ccccgctctc atcaggctca
     7861 gcgatgacct ggggattctt cggcaaaaaa cgcagcaaaa cataatcgcc gcttccgatg
     7921 ttggccaggt tcatgctgtc accggaaacc ttgatgaccg ccgtcccatg agttgaactc
     7981 cgaaaaaagc tatgaccatc caccgacacg atccgatacg gcttgtcttc aataaagacc
     8041 tgctccacct ccaccatacc ttctatggga gtattcaaca ccccgcttgg gccaaaatcg
     8101 ccagcattga tcgattcgtg gatggagaaa agcccgacac gatcagagcg tacctgctgt
     8161 tcagcatcct ccctgctgtt gtttgtggcc atctcagcaa acagggattt gttttcgggg
     8221 gtttcggcgg gcagcggcgg aatttgatat ttttcagcgg cctgatgaag cgcatcatgc
     8281 atttccttgc accgtgcatc gtaccaatcc aacctcgatt tttggttctc cttatcacgg
     8341 atcgctttga aacgtttttc ggcttcgcgc catgagttga tggcttccac ctctttattg
     8401 ggcaaaagcc agtccatgca gcctttcatc cataaggcaa cagcctgatg gtgaaatgaa
     8461 tttaaatatt tggtggaggc ttcgctgaac aatctttgag cctccctaaa gttacccatt
     8521 tctgcgacac tcaacccgca ctgtaggcaa atctccgcgg gctccaaccc atcagaagcc
     8581 tgccgcaaac gatcaaggtc ctcgataatg ctccaggcct tcttcatctc ttttctgtca
     8641 cattccctcc ttaatcgctc caacaaaaga ttggaggggt cactcaatga gcttttatta
     8701 aatttattca accacccgct gacatatttc aaccagcgga tggatttggt ctcagtcgtc
     8761 atcatggttt ctttccatcc tttttcgcgt ttttgtctgt gttttgctca ctaccctcag
     8821 agacggaaag cgcctccagc acctgtgcag tgcgcatcaa aataatattg cggatactgc
     8881 gatctttatc ttcttttgcc aaatcaatat catcaaaggc gtggacaatg ctcatcaaaa
     8941 tttctgcctg tgcctcagca tggccgatct cctgatacgc gttacgctgt gcctcgccat
     9001 aggcgcgttt aacatcagca tttcccatcc agccagcctt ccatgtatca atgcgttgtt
     9061 cagaaacggc tttattgtcc acttcaaagt gcccgatatc acaccatagc agttgggcac
     9121 ctgcatcctt taatttatcg cgggtggatg aggttttcaa ttgggcactg atctccttgc
     9181 gtgcatcacc atcggtatat cggggagccg tgatctgatc cagctgatgc tttgtaatgt
     9241 aatccgagac tgctccagag atgacgccct ttaccatgtc atgccaggtg ttcggtcccg
     9301 acttcgatac ggtgcggtta taagtcaggt tgcggatcgc ttgaaccgtg aacgggtacg
     9361 ggttggtcaa acttcgcccg gctgcagtat taatctcacg gtgaccagac catacgcgat
     9421 agcgaaactg cacatctttc actgtcacca tgatcccatc cttggtcact gccgagatcg
     9481 ctgggatcaa cccgtgctgg tcatccagat taagggtgct gacaatcgtt tcaaagcgcg
     9541 acaaaaagaa cctgcccgca gaatacaccc cggcaggccc atgaagcctt tcggtcagaa
     9601 tcgcattgcc ggggccaatg aaaacatagc cagggccgcc gatctggtca atgcgatttt
     9661 ttccatcacc cacaacgtaa ttttctgaga ttttgatctt cgggtatttg attccgcaaa
     9721 atgatgaaag cagatacaga accgccaaca cataatgctt gatattgaat acatcctgaa
     9781 catatcgtgc ccctgacaaa aaagcactga tcaatgccgc caaagggagg atcaagaaac
     9841 gtgaaatggg ctcgccagtc aaaagcggcc gtacatagac aaactcgaac caggagagcg
     9901 gcccgataaa tatggcaaac acgatcacat accaaacctg tttcagaggc ttcgcccgtt
     9961 cctcctcata tcgttcaaca aggatgtaag cgctcaaaag aatggaagca gccaaccaaa
    10021 tcatcgggaa ggcaatatca ttccaaattc gcaaaagtgt gagtttatcc gctgaaaacc
    10081 cggccgcaat aaaaacagag accgcagccg ttgcaaaaag caaaaataac ccgcgaagtc
    10141 gtgcccagat caaatctccc accagaaggt tttgaaggat gcgcgagacc cgtgttttgg
    10201 cagaacggaa aatcatcatg gcgtaccttc ttcgccaatt acagggtttg attcgtctga
    10261 ttttttattt tccggcaaaa gccaatgccg caaattggaa agcatctgaa ccgtatcagc
    10321 gggcaacaaa cgctgtgttg ccgggttgat cgctgccgcc tccaaagcct gatacaaacg
    10381 caacgccaaa gcctcttccg tatattgatt ctcgtggaaa atccgcgaaa gggaatagat
    10441 catatcctgc tgggtccgcg aacgttcatg gttatgaatt cgcatggcgc gcagttcatg
    10501 gttggccaga gtcttttgag cctcctgctg ccaatgtgcc cgccaatttt caaaaagttg
    10561 agcacggaca tcttcattaa ctggaacaag gtcaccaaac ccggcactaa ggaccttaat
    10621 gccacgatct cgaaggatcg cagggccttt aaatctcaaa ggtggtgaaa acttcaaatc
    10681 ggcctcgttc acaaactgcg cgtctttgat cggggtttca tccgcaagca tcacaacctg
    10741 ataccccagc atcccctggt tggtcaccat ccgcccgaat ttccttttga agtcacccat
    10801 tgggaactca ttaggttctt ctggtttgta aaggtcatcg taattctcgt gcgccaactg
    10861 ttcccgaaaa atatcagcag ccacatatcc gggtagttcc gtccagtcga tcaactggga
    10921 ttcgccagta gcactgcggg cacgcgaata cacagcagaa aacacccgct ctttttcaaa
    10981 aacaaattgt gaagccgctc caaccttcac aatgcggatg tcggtatcac tccactcaaa
    11041 ttctttttcc gtaaaggcat gctccagttc gttcatatcc tgttcattga tggtatccac
    11101 gggctgaaat ttcacaactt gatcccccgg gcggggttca ggcctgtcca ccaggtcttc
    11161 agcagatggt gccgtaatct gcactgcccg ccagcccaaa tcggtccgcg taactttcaa
    11221 aatatcctcc gcctgcccaa gagtgaaggt tacgttcaca ttggttgtaa tctcaattcc
    11281 atcccgcgtg caggctttca ctccttgcac agatctactc tgctttctca gatccacaaa
    11341 ccccacgatc ttttccccgg attcggtaaa gacgacaccg ggaccaaatg ccttcacaaa
    11401 agtgacattg acatccttcc cagcgactcc caacttagcc gtaatctgct tccacatccc
    11461 aggccgttgc agcccttgaa catcctcctc ctcagcttca aaatgcacct cctgccgcaa
    11521 cgaatcaatg gacccagtct gttgttccaa aacgatcgca ctatacagat ccaccaaagc
    11581 cacaccagcc ttatggctgt ctaattcatg ttcctccatg ttcggctttc ctgcttctac
    11641 aaagatcgcc ggtccatgcc attttttccc tgccaaagaa cggatcaacc cggtaaacgc
    11701 tttttgagtt tgcaccaaac cgcgtacagg caaaataaac ggagataccc aaaacagcac
    11761 aacaaaaaat atgagaacaa aaacggaaat attggcaaaa agcatcgaac tggcgaggct
    11821 agcctctgga gaaacataca ccgcatcagc tgcgcgcaaa atataaacat atccccatga
    11881 aatgaccaac tgccagacaa gttttatttc gatgaagaaa gacaaaccag acagagatat
    11941 gaccaaaacg gcagttccaa gaatcgaaag cttcgcctgc cgtgagatcc gtgtatccgt
    12001 gggcatgata ttcatccgtc ccccattgag ggattcaaga cgcccaataa atcatcaacc
    12061 tccaggttgc tatatttgat ccccagatcg cgtcgcatat tggcaacaag agcaaacagc
    12121 ttctcgttgg ttacgcgttg ggctttccca tgtacagcca cactttcgcg ccaatcacga
    12181 aaagcacgga ccacttcatc cgaaccgatc aaaacgatct gtgaggcaat ggctgtttcc
    12241 gcctcctgcc tgtgatccgt ttcaaaggta ttaagagaag gggcttgcag cagttttgca
    12301 aactccgtat agacacccca cttcttgtcg ttaaatcgtt tcaaatattc ctgttcgaga
    12361 tctgcttttg cctgtcgcca aaaccccatg aaagtaaatg cacctgttgc cagggcaatt
    12421 acgaacgcaa ccacgatctc tgccgctaac ccaccgatgt tttccatatg aagactcccc
    12481 gataactgat tgaaaatgtg gatagatacg atgctcaaaa atcagtatat acacgcaacg
    12541 tcttcattgt caagaggggg gatttttgga tgcaattcac catctcacaa aactcaactc
    12601 caaataggcg aagtgtgtca taattatgga cagtccgtaa ctttttccgc acaccagcga
    12661 ctatatataa gatacctgta tttcgccaac ataaaggaga caagcatgaa aacaagaaat
    12721 ttcttgattc gcatcggtct ggcactattg gttgctgccc tattgggagc aggggcaggt
    12781 ctactagcac gtgaaaaggt ggatgccgca gaggggacat acaaaatcct ggcttggaat
    12841 gacctgggaa tgcactgcta taaccgtgac tttgcagacc tcgcagtatt acccccatac
    12901 aacaccttat gggttcaggt gatcaaagct ggcgatccgc cgactctggt caccagcggt
    12961 gttaccgtgg aatatttcta cgaagacaat acatactcgg tcgggaagac caatttctgg
    13021 tcctactcga aagacctgtt cggggttaac ctaccgccta atgtcggtct gaaaggaaaa
    13081 ggtctttctg ggaccatgga tttcagcggc gaccattttg tagcagaagg tatccctgtt
    13141 accgaattca gtgacagcgc ccctaccacc agccaaccct atcagctcgc cactgtcatc
    13201 gtaaaagatt cagccaccgg cgcagaactg gcacggacaa gggtcgtcac acctgtttct
    13261 tcagaaatgc gctgtgataa gtgccacagt gacggcggtg aagccagccc cgacaatcca
    13321 acgggaaagg tcgaaaccaa tattttgcag ttgcatgatg aagaagaagg cacaaattta
    13381 atggccaatc gcccggtgtt gtgtgcagat tgccatgcat ccaatgcact tggcgcccct
    13441 ggacaatcgg gaatacccaa cttatcaaat gcaatgcaca acaagcatga ggatgaatcg
    13501 cccaatacgc tgaatggctg ctacaactgc catcctggcc cggagacaaa atgtctacgc
    13561 gacacaatgt cacaagaagg gatgacatgc attgactgtc atggcggcat gtctgatgtt
    13621 aaaaacaacc ccgatccctg gctgaatgag ccgcgctgcg acacatgcca cgacagtggg
    13681 caatacgacc aaaatcatcc gctctaccgc ttctccaccg aacacggagg cttgtactgt
    13741 gaagcctgtc atgacagcac acatgcggtc gcaccaagtc gtgaagaaaa tgatgcgatc
    13801 aaatttaata tcctgcaagg caagaatggc ccggtagacc aatgtacggt ttgccataca
    13861 accactccat caacaggctc agggccacat gggctcacct cttcgggagc gctattcgaa
    13921 gatgtaccct ctacatattg ggcgcgggat ttcattgaaa gactgtataa cgcaggcatc
    13981 accagtggat gcagtacctc cccgctgatg tattgcccgg acgcaacggt cacacgcgca
    14041 cagatggcaa tctttattct gcgcggcatg catgggagcg cctatatccc acccactgca
    14101 tcgggaactg tcttcagcga tgttccagca ggttcctttg ccgcagattg gatcgaacaa
    14161 ctcgcagccg aaggtgtgac cgcgggttgt ggtggcggca attactgccc ggacgccacc
    14221 attacacgcg ctcaaatggc gatcttcctt ttgcgtggcg aacatggaaa tgcatacacc
    14281 cctccagccg caaccggcac cgtcttcggt gatgtgccgc ttggttcatt cgccgacgcc
    14341 tggatcgaac aactcgcagc cgaaggggtc acaagtggat gcggcggtgg aaattactgc
    14401 cccgacaact cagtgacacg tgctgaaatg gctgtattcc ttgtaagagc attcagtctg
    14461 ccataacaga cattgaacac agaaagcgcc gcgattttct cgcggcgctt ttaatttcaa
    14521 gatcaccctc caaacaaatt tgtgaagcaa aaaataaaaa atatgacatt ggtattattg
    14581 aacagctcat gaaaagttgt atattgaaaa tgtgttgagt ttatatacaa cctgggaggg
    14641 ttgacatgaa taccaaaaga ttgttttccc gcataatcgc catcctaatt gtcgttatat
    14701 tctttttatc aggcgctgca ccaacgctgg ctgcgcctgc tcccattccg ctttacgcag
    14761 acgatccaac accgcccgct caaaccgtca aactcatctt catccaccat tccacaggtg
    14821 agaattggct ggccgatgga tacggtaatc ttgggatcgc tctggaacag aacaactatt
    14881 ttgtcagcgg cactaactac ggctggggac caaatgccat cggtgaccga acagacatcc
    14941 cgaattggac cgaatggttc cgttccggag atacagagac gtacatgaac gccctgttca
    15001 acgaaagcgg acaaaactcc tccttcaccc gaaacctgac cgacccaggc ggagagaacg
    15061 aaatcatcct tttcaaatcc tgttttccaa attccgcgct tgaaggcagc ccaaccgacc
    15121 cgccatcacc cgatggctgg ctgacagtag gacatgccaa atacgtatac aacgagatcc
    15181 tgcagtactt tgcatcccgt cccgacaaac tattcatcgt catcactgcc ccaccattgc
    15241 aggatggcac ctacgccgaa aatgcccgcg ccttcaacga atggctgatg aacgactggt
    15301 tgaccgaaaa cggttatacc ctacccaatg tggctatctt tgatttctac aacgtgctga
    15361 ccgggccaga caatcatcac cggtacatca atggcaccat cgaacatgtc tttacgtcag
    15421 gcatgaacac atcctactat ccatcatctg ctgacgacga ccatcccagc gcagcaggca
    15481 gtcaaaaagc gacagatgaa ttcatttccc tgatcaatat cttctacaac cgctggcgaa
    15541 attcaacttc atccaccttc accgatgtac cttcaacata ttgggcagcc gcctatattg
    15601 aaagactcta caacgccggt atcaccagcg gatgcagcac ctcgcccatg atgtattgcc
    15661 cgcttgcccc tgtcacccgc gcacagatgt ccatcttcat cctgcgcggc atgcatggaa
    15721 gcgcctatat cccacccgcg gctacaggca cagtattcag cgatgtgccc gctgatgcct
    15781 tcgcagccgc atggatcgaa cagtttgcca gtgaaggcat caccagtgga tgtggaagcg
    15841 ggaattactg ccccgatgcc aacgtcaccc gcgcacaaat ggcaatcttc ctgttgaaag
    15901 gtgagcatgg ctccagttat gttcccccca cagcaacggg tattttcacc gatgtcccca
    15961 tcggctcctt tgccgcagac tggatcgaac aactcgccaa cgaaggcatc accagcgggt
    16021 gcggtggcgg aaactactgc cccgatgccg atgtcacccg tgaccaaatg gccgtcttcc
    16081 ttgtcagggc attcagcctg ccgtaatttc ttgacgcatg ccttttgcgg tggtatcctt
    16141 gcgcgcaatg ttcagccaga ttgtagttga caccactacc aataccactc gtaccaataa
    16201 tacagggacg gggcattggt ggttgtcagg tgaacagcaa caagttcata ctcactaggt
    16261 acaccaaacg ccccgaaaaa aattcggggc gtttttctta cacatcaaaa aggagaagca
    16321 atcatgtcca tttcaaacgc cacacccgtc ctcgcccttg acgagaaaaa cctcatccct
    16381 gtcagcgatc acctcaaaca gatggccgat attccccctt cacgcatgtt cctcatcaac
    16441 aaatcgttga aggtctatgt ggaaaagaac gcaggcgcga aaatattcga cgcttcacaa
    16501 ggcgatggag gcgcttcact ccccggcaca ccgaaagcga ttttggaacg cgcgcttcaa
    16561 cttcaactgg aacatggtac ggcttacgat atgccattcg gcaccgaagc atatcgcaag
    16621 gcggtcatcg aacaatattg gaaattagac tcatcctctg gttgggggcc tgcgaacgta
    16681 ctcggcaccg caggcgggcg cgatgcactg gtcaaggtct atggagccat gttggccctc
    16741 gggcatggac ggcagggtga tctcattatg gtctcccgcg tcccatggat ctcctacaat
    16801 tgggggccat acggtgtcgg ggcgaacgtg ctctgggctc ccggcgaccc agcccaaggg
    16861 tgggcttatt cggaagacgc catccgcgaa agcgtcaagt tcgccgaatc caaaggcaga
    16921 aagatcgcag gcattgtcat caccaatcca gataacccca ctggtttgac aattgcggtt
    16981 gaaaaacagg tttcactcgc caaagccgcc ctcgaagcag gtgtagcctt cgtgctcttc
    17041 gactggatgt atcactacgt tacagacgaa tcgccgatgg atctgaattc ctttctcaag
    17101 aacttcaccc ccgaagaacg caaacgcctg atgtttctcg atggcatcac caaatcgctc
    17161 ggcgggtcga acatccgcaa ctgccatttg atcgccgacg aagcagtgat caaattcatc
    17221 atggcccgcg cttcacatgg tgtcattcct tccttctact cgctggccgt tgccatggcc
    17281 gcctacgaaa tgggattcac cgaagccgcc aagaccatca tcgaaccaac caatgccagc
    17341 cgcgccgtat taaagaaatt gctcgccgaa agcggaatgc agcacattat tggcaagggc
    17401 tattacgcct tcatgaatgt cggtgaattt atcaaagcca agggttgggc cgacagcgaa
    17461 ccgctcggcc aataccttgc cgaaaatcat ggcgttgcca tcgtccctgg cgccttcttc
    17521 tccccctttg gtggagattg gatccgcttc tcgtatgcca ctcccaccga aagaaccgaa
    17581 ggcgcattca aacgattggt ggaagggctg gagagtttga aaaaataaaa agggttgaaa
    17641 gttgcaagtg gaaggtttaa agtaaaaaca cttccacttt ccacttccca tttacaaatt
    17701 cgcacccaat taggatcatc tcatgcccca acaatttgcc gataaatatc tcctcgccgt
    17761 cgaacaggcc atcaaggaaa agccgcaagg tggtctcgac ggtttcgagc aggaatggaa
    17821 tttgctcgat gaagaactgc gcccgttact aaccgttggc gcaggaccca gccaacactc
    17881 gtttgtggat tatctgcgcg ccgagtgtgt tcctcactgg catcaacaat tcagccagct
    17941 cgaagtcttt cactggatgg tggagtgggc cacacgtccc tactacaccc cacgcggagc
    18001 gatgtacgaa gcccgcctga tggaagccac tttgatgaat gccctgcacc gcgcaggggt
    18061 caactttgga gagcgcctgc actactggca tggcaacctg ctcttcctta ccgacatcgg
    18121 gcatcaatcc atcccgggaa actggtcgct tgccaaacgc cgctatctcg aaaaatgcgt
    18181 ggacctatac ggtgacactc tcgccaccac aggcatccac accaacatgt cactgcccga
    18241 tccgctcttt gcatgggatt tcatgcacct ttcccccagc gaacgcggcg accaacacct
    18301 tgatgaattc aaatcggagt tctatatcac tgctacacgg ctgctccgtg ccttcgcatc
    18361 tctcttcatt gcaaccagcg catccacacc gatgcaggca caggtcaagg acggacgcgc
    18421 agcggtcatc ctgaccgagt ttgattcgat tcgaaatctc actttcccaa atccacgtga
    18481 gatcgatctc cccgatctat atcgctcata caacgactac ctgcaaattt catacgacct
    18541 cgtgcggcgc ggtgtgcgtt tcggcaacaa caactggact cccgtacgcg cgcgcagttt
    18601 ctccgaacct gtggagcgga tcatctccac gaccagcgat caactgagtt cactctatgc
    18661 acgcggctta ttcgccgccg ggcaatcaac cccgcccgaa gaaatggcgc gacaagtgga
    18721 acggcagaat cttatggcgc gaatcaacct gcccatgggg cgcgtcgaag tccgtgtgga
    18781 tgagggcggt cacagcctgg acctcgatat tgccaacatg accttcaggc atttactgct
    18841 cctgaaaatt tactccgacc cgcagtttgc acgtggattc cgttacgacc gtgaagatat
    18901 cacccgtgcc cgcgccaatg aaaacatggc cgccaaacat ggtctgcgtg ctgagatcga
    18961 aaacccgctg accggcaagc ccattaccgt gcgcgagttt ctcaaatgga cactgaacga
    19021 actcaaaccg ctggcgcagg gattaaacat gtgggacgac ctgcatccac ttgtggaaat
    19081 gtcggaaggt ggacgcaaca cttccgaaaa aattcgtgcg cgcctcaaaa tggagatcgg
    19141 tgacgataac gaagtaccgc tcccgctctt gaaggaattc ttctacgaac atgaagcaca
    19201 ggtgaaagcc gatgtggaaa ccatttgcgc caaccatgct tcactgggcg ccgattctgc
    19261 caagatcgcc gagttcattc aacgtagccg cgacacggtt cgcagttccg aaattccgaa
    19321 tgcacctgtg caattccgtc cgcgcgcgca ggctgtgatt gaggtatcgt atccaaacaa
    19381 aaccgccgag atccttgatc ttgcccagca attgattcgc atcccctctg tcactgcctg
    19441 cccggtggag cgtttcgaag aagttcaccg tgctggttca ttaattgacg attacctgcg
    19501 caatgcaggt ctggatgtga aatacttcga cgggaaatat ccagccattt acgcgacctt
    19561 ccccggcgca tccaaaccga acccgatcct gctgactggt cactttgatg tggtcgaacc
    19621 cgaacccgac gactcgcaat tcaatccacg tatcgaaggc gattacctct tcggccgcgg
    19681 cgcagcggat atgaaaaccg ttgttgccac atatttggtt tggatgaagg atatgtgcaa
    19741 gagcgcatcg cgccccaaca ttgccttgat gctggttggc aatgaagaaa acggcgaagc
    19801 cgaagcctgg ggcacgccgc atgttttgaa agagctaaac ctcacaccct ctctcttcat
    19861 tgctggcgag cgcaccggcg aaaagggaaa tgaactcttc ggcgaaatct gcgtggagaa
    19921 tcgcggcgtg atgcgctttg acgtgatcgc gcgcggtgca aaaggtcaca gcggcgtggc
    19981 agggactggc gatttgagcg agaagctcat tgctgcacgc tctgcactga atgagatctt
    20041 cgcaagccat ctcacactca aagcggccga tggctggcaa tcacaagcca aattcccgtt
    20101 tatcacggtc ggcacaccgg gcgtgtacaa cgtcactgcg gcagaaggga tcctgggtgt
    20161 ggagatccgc cccattccac aggatgatgt gactggttta cgcttgaaag ttgaaagtta
    20221 ttgtgctgaa aatggtctcg aagcaagttt caacgtgatg gaaaacggcg tggcttgcga
    20281 cccgaataac ccagcgttga aagcgctcat tgaagcagtg aggcaggctt cgggtggaga
    20341 agaaccactc atcggaaaga agttacccgg caccagtgca agattcgctc ctggcggtca
    20401 agccgtggtg tggggccaat cgggcgtggg tccgcatgcg aagaacgagg cgcattacat
    20461 cccaagcatc gaaccgtatt acaactcgct gaatgaactg gcgaagttgt ggaaataaaa
    20521 acaaaggcga ccttgaacag gtcgcctttg tttttatggc aatggtttta taagcaaaag
    20581 atttggctac ccatctgcat aattggcaac gatcaacttc agtagctgct cgttgaacct
    20641 gcgggcgtca gcgccgagtt gactgaaaaa ttttcggtca attgcttcca ctgcaggcac
    20701 ggcctgcccg gccaatttgc gcccctgtgc agtaatggac aatgcctttg ctcgtgtatc
    20761 cgtcgggtgc ggctttcgca ggatcaaccc tttttcctcc aacgtgcgca acacattgga
    20821 agtcatcatc acatccattt tggcgtgatt ggcaagatcc acctgtgtaa gaggttta
//
LOCUS       DAOQJC010000065        20735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1171_length_20735_cov_9.510058, whole genome shotgun sequence.
ACCESSION   DAOQJC010000065 DAOQJC010000000
VERSION     DAOQJC010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 20735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20735
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1171_length_20735_cov_9.510058"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(352..2445)
                     /locus_tag="PLE39_11130"
     CDS             complement(352..2445)
                     /locus_tag="PLE39_11130"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNE68927.1"
                     /translation="MPIHTSTNYWVLETRHTAYAFGLNNAKLLTHCYWGTRLPFIQDY
                     PAPFNPVHWASTDGFDHFVPEEYPGYAGTKYTDPCLKITFADEVRDVVLEYQDFEISA
                     SPMPTLALRLKDSFYPLQLTLHYRIHEEYDLIERWVTFENLGDQPMLIERAFSAKWTL
                     PSGDGYSLTHMYGRHVGEFNLQREELTPGLKVIESRRIIPSHRANPWFAVDRNASEDH
                     GEVWFGTLAWSGNFKTTAEVTEFASTRISIGLNDWDFAWKLMPAQSFSTPASISGYTQ
                     NGFGGASRLFHDYVRNEIIPHGKVPHKILYNSWEATLFNVDESSQTALAEIAAQMGIE
                     LFVMDDGWFHNRNWDNAGLGDWFPDKVKFPNGLSGLIKKVNDLGMEFGLWVEPEMVNP
                     DSDLYRTHPEWVIHFPTRARTESRNQLILNMAREDVQNYIITTLDTLLRENNIAFIKW
                     DMNRGVSEPGWPQAPGDQRELWVRYVQGLYHVWGTLRQRHPNIIWQSCASGGGRADFG
                     ILQFADQVWVSDNTEATSRLNIQEGYSQFLPANTMEAWVTDWGREIVPLEFRFHVSMC
                     GSLGVGGNLLEWDQAHRESAAGYIRLYKEIRHIIQFGDQFRLISPQNNDYSAVQYLSK
                     DKTEGVLFTFRTHIPDPFNIPMIHLRGLEPEALYSIEGFEQPRSGQAWMQAGLKVELK
                     NLQSKILRIQRLTPN"
     gene            complement(2455..3462)
                     /locus_tag="PLE39_11135"
     CDS             complement(2455..3462)
                     /locus_tag="PLE39_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNE68928.1"
                     /translation="MASLKKSPTIYDVAEVSGVSISTISRVLNSPDKVNPKTRQQVMD
                     AIDKLGFVPKAEARARAMRNTNRIGVLTPFFTAPSFVQRLRGIAGALSKENYELVVYT
                     VESAAQLENYFASIPLTSNLDGLIIMSLPISEANARRLTQYDIPTVFIEYPHPTLSSV
                     EIDDVAGGRMAAEYLISKGHRRIAFLGDTDLPEYAIHPVSLRLSGFRQTLQNEGIEIP
                     DEFVRLAPYTQEQTLRVANELLNLPNPPTAIFAATDFQALGVLKAARQMKVQVPEQLA
                     IVGFDDLDLADYADLTTIRQHLDESGRLAVEILLSRISDPTRPAQHIHLPLTIIERQT
                     A"
     gene            3625..4161
                     /locus_tag="PLE39_11140"
     CDS             3625..4161
                     /locus_tag="PLE39_11140"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNE68929.1"
                     /translation="MTFIPQPPQIPLLLKLGIYVSEKITGRIMLPPRILAWYPKAAIG
                     SGIMEALVAHEDGRMNQRMLKLIRMAASYAVACPFCVDMNSYEYRKHGITDEEAESLR
                     GDYESVASFSERERLAIAYTRLISATPLKFHPEMVDKVKAAFSEREFVILASTAAQVN
                     FWARLIQALGIPPAGFGE"
     assembly_gap    4204..4303
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4386..4901)
                     /locus_tag="PLE39_11145"
     CDS             complement(4386..4901)
                     /locus_tag="PLE39_11145"
                     /inference="COORDINATES: protein motif:HMM:NF023621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HNE68930.1"
                     /translation="MSFFSTILQKLGLKKEEPAATPPAAAPAQTTSTPAPAKPPVGYG
                     PTTTASTAAAPKPAGAGTLSDRRVQDDMAVNHEPMKPAVKAISEVDVVKHLESLGAGS
                     GLDWKVSIVDLLKLLDIDSSREARNELAKELGCPEDLMHDSAKMNVWLHKTVLKKIAE
                     NGGNIPASMLD"
     gene            5057..5851
                     /locus_tag="PLE39_11150"
     CDS             5057..5851
                     /locus_tag="PLE39_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68931.1"
                     /translation="MENKKTPFWTRDSSILLGGFFVTIFLIVYIWWPLAEEVLSYIDW
                     NGPWWLYMDWLLLGVFLFMSITIVARANLKTDVLIVFVGICGGLAIESWGTQTNLWHY
                     YTAERPPLWIIPAWPIASLSIDRITRLLSWMIERFQIRFKRSIHHSAFSILYWIIFVS
                     FLTLMLVFVSPTFDKSYTWLATILCILLILTPTDHRLALLTFIAGAGLGYFLELWGTT
                     RECWTYYTFQKPPLFAVLAHGMAAVAFWRAGLMVKMVMGKMGLVRE"
     assembly_gap    5862..5961
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6173..6862
                     /locus_tag="PLE39_11155"
     CDS             6173..6862
                     /locus_tag="PLE39_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE68932.1"
                     /translation="MPRVVKEEDYVARRNEILDVARQLVYTKGYEQMSIQNILDALNI
                     SKGAFYHYFDSKQSLLDGLIERMLDEAEMVLRPIVESKDLSAIEKMRRYFDTAGRWKV
                     AQKAFMLDLFRVWRADANAIMRQKQEAASIERIGPMLTTIIQQGVDEGVFSTRYPEQF
                     GNIFVGLSHGFEDKFVELLLTDHPPPDAVKRLEALIGAYSDSVERILGAPPGSLPLGD
                     IETMKEWLPKP"
     gene            6910..8136
                     /locus_tag="PLE39_11160"
     CDS             6910..8136
                     /locus_tag="PLE39_11160"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNE68933.1"
                     /translation="MQLYLRLAWRNIWRHKRRTVIIVLAMSLTLALMMWYDGLMNGFT
                     DAIYGNAVKILGGNVQVHAEGYRAEANSNPLFPLTDPQAVIDAAEANPLTLAATQRIS
                     TGGLVTSREGAFSVGIIGIEPEKELTVNIIGQNVKEGRNLTSADLDNVLIGKGLADAM
                     GVKVGDRITMVGRSQHEQMRQRTMTVVGIFDLGLADIEKQSVYISLGEAQALYEVTGS
                     TEVAIFLEKLGQESDVIEALQPSLPGYEIESFQANYPDLASAINTKGGVMDVFSVIII
                     AIAGVGILNLLLMAVYERTREIGVLGAMGLKPSQISMLFVLEGIMIGLVGVAAGIILG
                     LAINGYLMKVGLDFGNMTQAASYMALIQSRAYPTWGIEKLPLRVSMILIISALAALIP
                     AAEAGRREPAEALHFV"
     gene            8175..9395
                     /locus_tag="PLE39_11165"
     CDS             8175..9395
                     /locus_tag="PLE39_11165"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE68934.1"
                     /translation="MMQILKMAFRDLGRNRRRSFFSALAVGGGLALLILMSSVVAGEM
                     GSAIESAINLQTGHIQIRAATYDENKSSLKWEDLVANPDEIAGKIAALDQVETATPRL
                     YASGFLSSGTQSAGAKITGIDPLSPASDPYREGVISGEYLNPEDRDVLLIGKPMADKL
                     NLKVGDSVSLSLNTADGNVQDQTFTVKGIYSTHTYAFDSATVFLPLAKAQAMTRTEDH
                     ASTIFVLLKDTEMTDAVVPALSVSSNLEIKTWKDLNALFVEYETFAQSYIAIFYMIIL
                     AISASVIINTLIMSVYERTREIGVLSAIGMRGGRIMMLFLAESSMLAVGGVVMGLIIG
                     VLATLYFNVNGFYIGDMGLSGMAIADTIYAKLTVENIVNLTIMTFVVTLLAGLYPAIM
                     ASRMQPVEALRAEK"
     gene            9404..10090
                     /locus_tag="PLE39_11170"
     CDS             9404..10090
                     /locus_tag="PLE39_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68935.1"
                     /translation="MEVVKIENVTRVYKIGKVETQALRGVNLSIGSGEFTALVGPSGS
                     GKTTLLQMIGCLDQPSSGHVLISGKDVTTLNRNQRADMRRGTIGFIFQFFALIPTLTA
                     YENVEMPLLLNGHSPKERHERVMDLLKAVDLTERANNRPDQLSGGQQQRVAIARALAP
                     KPTLILADEPTANLDTANGKQVMDIMKKLNKETGVTFVFATHDPRVISYADRIVTLND
                     GLIEKDSSSS"
     gene            10193..10975
                     /locus_tag="PLE39_11175"
     CDS             10193..10975
                     /locus_tag="PLE39_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE68936.1"
                     /translation="MRKTLLIVFVILATALTACGGQATPEAVPTISLDAPSQSSGGSA
                     TASAVVVPVTKVELSFPLSGAVKTVEVAEGDSVTAGQPLVTLDTAILEARVAEAEANV
                     VTVTTQLTYLKRTGTSQERLDAAQANIDRAMAAVEIAKAQLSQATLVAPFDGTIASVE
                     ISPAEFASPGQIIIVMGDLTHFQIETTDFSEKDIPSVSIGQAAQVFIEALDQEFTGKV
                     VDIARSSETVGGDVVFKVTIQLDEQPDGLRWGMSADVKIEEK"
     gene            10986..11786
                     /locus_tag="PLE39_11180"
     CDS             10986..11786
                     /locus_tag="PLE39_11180"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNE68937.1"
                     /translation="MKKMIWMTFLAATLILAACGTPATPTPPPIQTEEPASPQNDFVS
                     SDAVVASAEVIPAQDSQISFVISAPVKEVLVKEGDTVSAGQTLMTLASPDLELAVTSA
                     ELELKAAELEFTYWIPARHDRPPERRQQAEAELEQARKKLETAKANLAQTSLLAPFDA
                     TVIDVNIQAGELAQVGKAVITLGDIAHMQIATTDLSERDIPNVKIGQAVNVYIEALDI
                     TVTGKVLHISPVSETVGGDVVYPVTISLDEQPEGMFWGMSAEVEIMIK"
     gene            11945..13330
                     /locus_tag="PLE39_11185"
     CDS             11945..13330
                     /locus_tag="PLE39_11185"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68938.1"
                     /translation="MTTITVIGAGSASFGENTLSALLRSKKLNGSTLRLVDRNAQSLD
                     IVHRLAARLNKEWRSGFNITAHTNHKDALDGTDFVVSAIEVGPREELWKADFDIPIKY
                     GVRQPYAENGGPGGFAHTARNAMPVLNIVRDMEQACPDAWFINFTNPMVRICDLVNRH
                     SKIKAVGLCHQIFIGYCFVGMVLAEELGIEVPKGITGMQADVVEHDLRDHVVHQTLPL
                     VDLRAAGTNHFTWILSLHDKRTGEDLYPLFRERFKAFDPSFEPLTREVFDAFDLFPVP
                     GDTHLCEYLPWLSDPVTKPWEKFNIRLYDWDVRANLRDVSLDRLNDMANGTVAIEGLL
                     NTDSEGALEMIENIAYAGNHYHLAANLPNVGQITNLPLGATVETPVHVDGAGIHPVHV
                     GALPEAVAELCRRELMVAQLSVDAAVEGDYQKALQCLLLDPVITDMHVAKRILDDYLL
                     TYKEHLPQFHG"
     gene            13674..14693
                     /locus_tag="PLE39_11190"
     CDS             13674..14693
                     /locus_tag="PLE39_11190"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNE68939.1"
                     /translation="MYICVLTSVPDENDIPYDPSPYMNGYRWKQHLVEPKNVEKQIRD
                     LMDEGVDVFINLCDGTPDDALSGIALVNALEKHNAAFTGADSKFFDPTRDEMKNAARR
                     VSVPTPNWLFVNRVEDAEKVAKRLKFPMLVKPPHGYASVGIRRNSRVETIEQLREQLK
                     LEIDEFGRALIEEFIEGREFTCLIAENPDDPKKPLTFTPVEFIFPNGESFKHYDMKWI
                     DYEKMSVAVVDEPKIQKTLREQTMRVFKELGGNGYARCDYRMDANGDIYMLEINPNCG
                     IFYPPHEPGSADFSLLNDPINHTKFMKLIVRNAQMRQTRIAAEKIIKRQTRKRPIAVE
                     QMAYS"
     gene            complement(14738..15907)
                     /locus_tag="PLE39_11195"
     CDS             complement(14738..15907)
                     /locus_tag="PLE39_11195"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNE68940.1"
                     /translation="MNTKTLEQSFLTHFNAKPAFIVRAPGRVNLIGEHTDYNDGFVLP
                     MAIDRAVWIALSPRMDSQVHIHSLDMETALPNDPQAVSTFDLNSLTKGKGWAEYIKGV
                     ADQLQKAGYQLHGFDAIITGDVPRGAGLSSSAAVELAAARAFAVTAGFEWNAAQMAAL
                     AQKAENEWVGVNCGIMDQMASAASQAGHALFLDCRTLEYQLPPLPKGIAVVILDTSTR
                     RGLVDSAYNERRNQCEEAARWFGVKALRDVSVAEFNEKTKDASELNVNVLKRARHIVT
                     ENERVLEAVEAMKLGNIQRLGELFNASHSSLRDDFEVTNEALNQIVACAQEQPHCFGA
                     RMTGAGFGGCAVALVREENAAAFTQAVSAAYKQRSGLEASIYVCKASDGAGVIQG"
     gene            15934..17499
                     /locus_tag="PLE39_11200"
     CDS             15934..17499
                     /locus_tag="PLE39_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68941.1"
                     /translation="MTELEILEKYEPVLRFAKSERFFPMAVEPYLEKCHIFPGGPQGV
                     LEIVMHIHEPMITRIGKLQSEQFFLRFINKPLNDADAWVWWGLLSAAGMVGGWLTANF
                     AGVEFMFVASLLGALVLFMLASPIRLRIIPAVLAAGAFITAEVTPIWFFLHPSRVMSV
                     AVEYLVLLPIYLVILFYLSMRTLKFILDRVVPEGPGVVMDMFSQATEKIAREAYFEYA
                     AMLAMDAQPVYYGRVLHEKDEDGCAWTILQYHFFYAFNDWRLAANGMNHHEGDWEMVA
                     VYLKNNEPYAVLFSQHGAGNIEKWKTVRKAVDANGHETTHPVVYVALGSHANYSKPVV
                     IRSPWMYKPGKLQQVLFWLDGFIHYLFLLLNPNQRAREIALREFWKQGGKFLTEEMFA
                     RLRDETDHYIVTLPMEIASGDGHRIGLSGQGSHEGFIRSSRYIKRVMSDRKTTKPQVA
                     EWRRVLLNPEPDWVQYKGLWGVKSMLGEESGPPGPKWDRPGKKQVGVQERKRWGRPLE
                     WLAELEKTVPDRK"
     gene            17526..18449
                     /locus_tag="PLE39_11205"
     CDS             17526..18449
                     /locus_tag="PLE39_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68942.1"
                     /translation="MSISLVLEVAVGLIVIYYILGSVVSLITQWINEFFESRGRALEQ
                     YLIKLVGDKKIGDLKNLPQLQSLRPIRFKGPLSVFGSVTEPKMIEKIPVATLVDAYFD
                     LAGLTAKKEMELLELAELVDRLPDSEGKQAFITWINQGVTSIAELRVRATSYFTGILE
                     QAAATFRARARSFVIILSIGITILFGTDTIQLARNLWTNAELRAIAAAQANAVVAREG
                     ASADLSDLIDDLSQYSIKIGWWQTQQFPEGNNVGDWALFIFLKVLGLGVTAAAVSQGS
                     SFWYDLLKKLTSPTSSSSGSKSSPSGGSSSG"
     gene            complement(18490..19392)
                     /locus_tag="PLE39_11210"
     CDS             complement(18490..19392)
                     /locus_tag="PLE39_11210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE68943.1"
                     /translation="MQKPPTFSLLEASNWRDYELLDSGDGQKLERFGKYIFSRPESQA
                     MWSRALPSSEWNNAHAVFQPTAEESGGHWIEKKKLPEKWEMKYPLPHVQDLTFSVMTT
                     PGRHLGVFPEVASHWDFMADSVRKAGRPAKVLNLFGYTGLASLAAAAAGAQVTHVDAS
                     KKSVGWARENQALSGLNEKPIRWIVEDAVKYVQREEKRGVKYDGIILDPPKFGRGPKG
                     EVWEVYKSLPNLLEMCRACLSDSPLFVIVTVYAVRASAIHVAQAMDEMMKKYNGNIDS
                     GELVTREKSANRLLSQAVYARWSK"
     gene            19550..19996
                     /gene="mraZ"
                     /locus_tag="PLE39_11215"
     CDS             19550..19996
                     /gene="mraZ"
                     /locus_tag="PLE39_11215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNE68944.1"
                     /translation="MFLGHFQHNLDDKGRLMIPARFRELLAGGAFITQGFDKCLMVMT
                     EDYFKQVYERIEAMNLADPTARLLRRLILSNAYPIEVDKVGRILVPQNLRAFLGIESG
                     ELVVAGQGEYFEVWTPAHWGEQMAQLQDTEANNARFSTLDLSKPAN"
     gene            20014..>20735
                     /gene="rsmH"
                     /locus_tag="PLE39_11220"
     CDS             20014..>20735
                     /gene="rsmH"
                     /locus_tag="PLE39_11220"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNE68945.1"
                     /translation="MQHKPVLYQEIIHALQPRNGGRYVDGTLGAGGHARGILEACTPD
                     GLLLGLDVDPQALALARETLAPYEGRIHLVQASHITLSQQLAALKWDSIDGIVLDLGA
                     SSMQFDNAERGFSFMQDGPLDMRFGINAMMSAEEIVNTFDERELADIIFRYGEDRDSR
                     KIAKAIVMNRPLRTTRELVAVIEKASPRHGDRVHPATQTFQALRIAVNDELAAVEKTL
                     PQAVAALRSGGRCAVISFHSLE"
BASE COUNT         4776 a         4662 c         5774 g         5323 t
ORIGIN      
        1 gcaggagcct cggcggcttc agtggcagcg ggttcttcgg cgggagcttc agtagcgggg
       61 gcgccgcagg ctgagagcag catggaagcc agaacgagca ggctcatcac agaccataac
      121 agggatttct tgttcattgt tctcctcttt caatttggtt ggaaataaga ttgcgtttgt
      181 gaagggcagg gccaggggga tcgcacgcac ctcctaaaac gtttgagaag gttgtctgaa
      241 agtgttatct tgaaagcact ttcaagtgac agtttagcac tttcatttgc aagagtcaag
      301 agggaaattt tctttttcgg ggagaaaatg tttgaggtgt tcgagcagtt atcagtttgg
      361 ggtgaggcgt tgaatccgca aaattttgct ctgtagattc ttcaattcca ctttcaagcc
      421 tgcctgcatc catgcctgtc cggagcgggg ttgttcaaag ccttcaatgg aatacaaggc
      481 ttctggctca aggccgcgca gatggatcat gggaatgttg aatgggtcag ggatgtgagt
      541 gcggaaggtg aacaatacgc cttcggtttt gtctttgctc agatattgca cagccgagta
      601 atcgttgttt tgaggtgaga tcaaacgaaa ctggtcgcca aattggatga tgtgccgaat
      661 ctctttatac aagcggatat aacccgctgc ggactcacga tgggcctgat cccattcaag
      721 gaggtttccg cctacgccga gagagccgca catgctgacg tggaaacgaa attcgagcgg
      781 gacgatttcc ctgccccaat cggtaaccca ggcttccatt gtgttggctg gtagaaattg
      841 tgagtagcct tcctgaatgt tcaagcgcga ggtggcttct gtgttgtcgc tgacccaaac
      901 ctggtctgca aattgaagga tgccgaaatc ggcgcgtcca ccgccgctgg cacagctttg
      961 ccagatgatg ttggggtgcc tctgacgcag tgtgccccag acatgataca agccctgcac
     1021 gtaacgcacc caaagttcgc gttggtcgcc gggggcttgc ggccagcccg gttcgctcac
     1081 gccgcgattc atgtcccatt tgatgaaggc gatgttgttt tcgcggagca gggtatcgag
     1141 tgtggtgatg atgtaattct gcacatcttc gcgtgccatg ttgaggatga gttgattgcg
     1201 gctttcggtg cgcgcacggg tggggaagtg gatgacccat tccgggtggg tgcggtagag
     1261 gtcgctatcg gggttgacca tttcaggttc gacccacaga ccaaattcca tgccgaggtc
     1321 gttgaccttt ttgatcaaac cgctcaaccc attggggaat ttaaccttat cggggaacca
     1381 gtcaccgaga ccggcgttat cccagttgcg gttgtggaac cagccatcat ccatgacgaa
     1441 tagttcaatg cccatttgcg cggcgatctc tgccagtgcg gtttgcgagc tttcgtccac
     1501 gttgaagagg gtggcttccc acgagttgta aaggatcttg tgcggaacct ttccgtgcgg
     1561 gatgatttcg ttgcggacgt aatcgtggaa gaggcggctt gctccgccga atccgttttg
     1621 ggtgtagccg ctgatcgaag cgggtgtgct gaaagattgt gcgggcatga gtttccatgc
     1681 gaaatcccaa tcgttgagtc cgatgctgat gcgggtggag gcgaattcgg tcacttctgc
     1741 tgtggttttg aagttgccgc tccaggcgag tgtgccaaac cagacctcgc cgtggtcttc
     1801 gctggcattt cgatctactg caaaccaggg attggcacgg tggcttggaa tgatgcgtct
     1861 gctttcgatg actttgaggc cgggggtgag ttcttcgcgt tgcaggttga attcgccgac
     1921 atggcggcca tacatgtggg tgagggagta accgtctccg gacggcaggg tccactttgc
     1981 ggagaaggcg cgttcgatga gcatcggttg atcgccgagg ttttcgaagg tgacccagcg
     2041 ttcgatgagg tcatattctt cgtggatgcg gtagtgcaga gtcaattgca acgggtagaa
     2101 actgtctttt aatcgcaggg caagtgtcgg catgggagat gcgctgatct caaaatcctg
     2161 atattcgaga accacatccc gcacttcatc ggcaaaggtg atcttgaggc aggggtcggt
     2221 gtatttggtg cctgcgtaac cgggatattc ttcagggaca aaatggtcga agccatcggt
     2281 ggaggcccag tgaacagggt tgaatggtgc gggataatct tgaataaaag gcaggcgtgt
     2341 gccccagtag cagtgggtga gcaatttggc gttgttcagg ccaaaggcgt aggccgtgtg
     2401 gcgtgtttca agaacccaat agttggttga ggtgtggatg ggcatgagtg cctcctatgc
     2461 ggtttggcgt tcaataatgg tgaggggtag gtgaatgtgt tgcgcggggc gggttgggtc
     2521 ggaaatgcgg gaaagcagaa tttcaaccgc aaggcggcct gattcatcaa ggtgctgacg
     2581 aatggtggtg aggtcagcgt aatcggcgag gtcaaggtca tcgaagccga caatggcaag
     2641 ttgttcgggc acttgcactt tcatctggcg tgcggctttg agcacaccaa gggcttgaaa
     2701 gtctgtggcg gcaaagatgg cggttggcgg attgggcagg ttgagcagtt cattggccac
     2761 gcgcagcgtt tgttcctggg tgtatggcgc aagacggaca aattcatcgg ggatttcgat
     2821 gccttcattt tgaagggttt gccgaaaccc ggataagcgt aaactgacag ggtggatcgc
     2881 gtattcaggc aggtcggtat cgccgaggaa ggcgatgcgg cgatgtccct tgctgatgag
     2941 gtattctgcg gccatgcgcc cgcctgctac atcatcaatt tcgacactgc tcaaggtggg
     3001 gtgtgggtac tcgatgaaga cggtggggat atcgtactgg gtcaggcggc gggcattggc
     3061 ttcgctgatg ggcagggaca tgatgataag cccatcgaga ttgctggtaa gtgggatgga
     3121 agcgaagtag ttttccagtt gggccgcaga ttcaactgtg tacaccacca attcgtagtt
     3181 ttcttttgag agcgccccgg caattccgcg cagacgctgc acgaaggatg gggcggtgaa
     3241 gaacggggtc agcacaccga tgcggttggt gttgcgcatg gcgcgggcac gcgcttcggc
     3301 tttggggacg aacccaagtt tgtcgatggc gtccattacc tgttggcggg tcttggggtt
     3361 taccttgtcc ggcgaattta gaacccgcga aatggttgag atgcttacgc cggagacttc
     3421 ggcgacatcg tagatggtgg gggatttttt tagagaggcc atgagtgaaa gaaatctgaa
     3481 agcagaatga aagcattttc agtttaacat agttatgagc agttgccaat gaaagcggtt
     3541 tgtctggctc tcccatttct ggcaaaatgg aaggcgagag ttgcgaatcc gcggttgcaa
     3601 attacacagg cttataatct ggttatgact tttattcccc aacctcctca aattcccctg
     3661 cttttgaaac tggggattta tgtctctgaa aagatcaccg ggcggatcat gcttccaccg
     3721 cggattttgg cctggtatcc gaaggcggcc attgggtcgg gcatcatgga agcgcttgtg
     3781 gctcacgaag atgggcgcat gaatcagcgc atgttgaaat tgatccgcat ggcggcatcg
     3841 tatgcagtgg cttgtccatt ttgcgtggat atgaattcat acgaatatcg aaagcacgga
     3901 atcaccgatg aagaagccga gtctttgcgc ggagattatg aatcagtagc gagtttttcg
     3961 gagcgcgaaa ggctggcgat tgcctacacc cgcctgatct ctgccacgcc tttaaagttc
     4021 caccctgaaa tggtggataa agtaaaagca gctttttctg agcgtgagtt tgtgatcctg
     4081 gccagcacgg cggcgcaggt caatttttgg gcgcggttga ttcaggcctt ggggattccg
     4141 cctgcagggt ttggcgagtg atcagcggcg cattggctgg ataaatccat tggcaagata
     4201 aatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncattggc aagataaatc
     4321 ttgccgtact taaataaaaa cagcgcggaa attaatccgc gctgttttta taaattggtg
     4381 tgaaattagt cgagcatgct ggcggggata ttgccgccgt tttctgcgat cttcttgagg
     4441 acggtcttgt gcagccacac gttcatcttg gcagagtcgt gcatcaaatc ttcggggcag
     4501 ccgagttcct tggcgagctc gttgcgggct tcgcggctgc tgtcgatatc gaggagtttg
     4561 agcaggtcca cgatggagac tttccagtcg aggcctgaac ctgcaccgag gctttccaaa
     4621 tgcttgacca catctacttc agagatggct ttgacagcgg gcttcatcgg ttcgtgattg
     4681 acggccatat catcctgtac gcggcggtca gacaaggtgc cagcgcctgc gggtttgggt
     4741 gcggcagcgg tggatgcagt ggttgttggc ccgtatccta caggtggttt tgcaggggca
     4801 ggggttgaag tggtttgtgc gggagccgca gcagggggtg ttgcggcggg ttcctctttt
     4861 ttgaggccga gcttttgtag aatggtgctg aagaaactca tgttcgtaat cctttccttt
     4921 caagtgtgtg atggaaaatt ttgattacaa cctgctaatc aatggcttca ttataacgtg
     4981 tgttatctcg aatgagatta aaaaaatagt acataagata tatcttattc tttgagtcgt
     5041 acaatataat ccgcgcatgg aaaataaaaa aacacccttc tggacccgtg actcatccat
     5101 tttgcttggc ggattttttg ttacgatctt tttgatcgtg tacatttggt ggccgcttgc
     5161 agaagaagtg ctttcttata ttgattggaa tggcccgtgg tggttataca tggactggct
     5221 gttgctgggt gtattcctgt tcatgtccat tacgatcgtg gcgcgtgcga atctcaagac
     5281 cgatgtgttg atcgttttcg tgggcatttg cggcgggctg gcgattgaat catggggcac
     5341 acaaacgaat ctctggcatt actacaccgc cgaacgcccg cctttatgga ttatccccgc
     5401 atggccgatt gcaagcctct cgatagatcg aatcaccaga ctcttaagtt ggatgattga
     5461 gcgattccaa attcgcttca aacgcagcat tcatcattcc gcattcagca ttctctactg
     5521 gatcattttc gtctctttct taaccttgat gctggtcttt gtttctccga cattcgacaa
     5581 atcctacaca tggctcgcga cgatcttgtg cattttgctg atcctgacac cgaccgatca
     5641 ccgcctggcc ttgttgactt tcattgcggg ggcagggctt ggttattttc ttgagttgtg
     5701 gggcaccacc cgtgagtgtt ggacgtatta cacatttcaa aaaccaccgt tgtttgcagt
     5761 gctggcgcat gggatggcgg ctgtcgcttt ttggcgggcg gggttgatgg tgaagatggt
     5821 gatggggaag atgggattgg tgcgagaata gtgggtagtg annnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn ngaatagtgg gtagtgaatg gtgagtgttt tagatccgca
     6001 ggatttcttg cggatctttt tgttttcggg gagaaactgt tcgagaaaat agaagcaggt
     6061 ttcggcgaag ggagaggcgc ttttttatcg ttaagaatcc tattgacaca ggcattcacc
     6121 cgatatataa tctcgaaaaa taaaccgacg gtcggtttat aaagaaggtg ctatgcccag
     6181 agttgttaaa gaagaagatt atgtagcaag gcgcaatgag atcctggatg tggctcgtca
     6241 gttggtttac accaaggggt atgagcaaat gtccattcag aatattctgg atgcgttgaa
     6301 tatctcgaag ggggcgtttt atcattactt tgattcaaag cagtcgttgt tggatggttt
     6361 gatcgagcgg atgctggatg aagccgagat ggtgttgcgc ccgatcgtgg agtcgaagga
     6421 tttatctgcc attgaaaaga tgcgtcgtta ttttgatacg gcggggcggt ggaaggtggc
     6481 gcaaaaggcg ttcatgcttg atctgtttcg ggtgtggcgg gcagatgcga atgccatcat
     6541 gcgtcagaaa caggaggctg catccattga acggatcggg ccgatgctga cgacgatcat
     6601 ccaacagggg gttgatgaag gtgtgttttc cacaagatac cccgaacaat ttgggaatat
     6661 ctttgtgggg ttatcgcacg ggtttgagga caagtttgtc gaattgctgt tgacggatca
     6721 tccgcctcca gatgctgtaa aacgccttga ggctttgatc ggcgcgtatt ccgattctgt
     6781 ggagcggatc ctcggcgcgc cgccaggttc tttgcctttg ggtgatattg agacgatgaa
     6841 agaatggctg cccaagccgt aattattttg taaagcgact ctcggatttt cgagagaaaa
     6901 ggagtaagaa tgcaacttta tctgcgcctt gcatggcgca acatctggcg gcataagcgc
     6961 cgcacggtca tcattgttct ggcaatgtct ttgacgctgg ctttgatgat gtggtacgac
     7021 gggctgatga atggttttac cgatgcgatc tacggcaatg ccgtgaaaat attggggggc
     7081 aacgttcaag tgcatgctga agggtaccgg gctgaggcga attcaaaccc gctttttccg
     7141 ctgactgatc cgcaagcggt gattgatgcc gcggaggcaa atccgctgac cttggcggcg
     7201 acgcagcgca tcagcacggg tgggttggtg accagccgtg aaggtgcatt ttcggtgggc
     7261 atcattggca ttgagcctga aaaggaattg acagtcaaca tcattgggca gaatgtgaaa
     7321 gaggggcgca atctcaccag tgctgatctc gataatgttc tgatcggcaa aggactggct
     7381 gatgcgatgg gcgtgaaggt cggtgaccgt atcacgatgg tgggacgttc gcaacatgag
     7441 caaatgcgtc agcgcacaat gacggtggtc ggtatttttg atttgggact ggctgacatc
     7501 gaaaagcaga gtgtttacat ttctcttgga gaagcacagg ctctttacga ggtgacaggc
     7561 tccactgaag tggcgatctt ccttgaaaag ctgggtcagg aaagcgatgt gatcgaagcg
     7621 ttgcagccca gcctgcccgg ctatgagatc gaatcgtttc aagccaatta tcccgacctg
     7681 gcttctgcga tcaatacaaa gggcggtgtg atggatgttt tcagtgtcat catcattgcc
     7741 attgctgggg tgggtattct caacctgttg ttgatggcgg tgtacgaacg cacacgtgag
     7801 atcggtgtgt tgggtgcaat gggtctcaag ccgagtcaaa tatccatgct gttcgtgctt
     7861 gagggcatca tgatcgggct ggtgggtgtt gcggctggga ttatcctcgg attggcgatc
     7921 aacggttatt tgatgaaagt cgggctggat tttggaaata tgactcaggc tgccagttac
     7981 atggccttga tccaaagccg tgcctaccca acctggggta ttgagaaact gcctttgcgg
     8041 gtttccatga tcttgatcat ttctgcattg gcggcactca ttcccgcagc tgaagccggg
     8101 cgtcgagagc ctgctgaagc gctgcatttt gtatgaggtg gttttgaacc acaaccactg
     8161 aaagagagaa gtttatgatg caaattctta aaatggcctt ccgtgatctg ggccgcaacc
     8221 gccgccgtag ttttttttct gcattggcgg tgggaggcgg gcttgcgctg ttgatcctta
     8281 tgtcgtctgt tgtggcaggc gaaatgggca gtgcgatcga gtcggccatc aacctacaga
     8341 ccgggcatat ccagatccgt gcggcaacgt atgacgaaaa caagtcaagc cttaaatggg
     8401 aagaccttgt ggccaaccct gatgaaatcg caggcaagat cgcggcgctg gaccaggtcg
     8461 agactgcaac gccgcgtttg tacgcgagcg gattcctctc atcgggtaca caatctgctg
     8521 gtgcgaagat caccgggatt gacccgttgt cacctgcaag cgatccgtac cgcgaagggg
     8581 tcatcagcgg ggagtatctc aaccctgaag accgcgacgt gttgttgatc ggcaagccga
     8641 tggcagataa gttgaacttg aaagtaggcg acagtgtgag cctttcactc aacaccgccg
     8701 atggcaatgt gcaggaccaa acctttactg taaagggaat ctactccacc catacttatg
     8761 cgttcgatag tgccacggtc tttttaccgc tggcaaaagc tcaggcaatg acgcggacgg
     8821 aagatcatgc cagtacgatc tttgtgctgc tcaaagatac agagatgacg gatgcggttg
     8881 tccctgcgtt gagcgtttct tcaaatttgg agatcaagac ctggaaagac ttgaatgccc
     8941 tgttcgtgga gtatgagacg tttgcccaaa gttacatcgc gatcttttac atgatcattc
     9001 tggcgatctc ggcttcggtc atcattaaca cattgatcat gtctgtttac gaacgcacac
     9061 gcgagatcgg ggttctttcg gcaattggaa tgagaggcgg gcgcatcatg atgttgtttc
     9121 tggccgaatc ttccatgctg gctgtcggcg gcgtggtgat gggattgatc atcggtgtgc
     9181 tggccaccct ctatttcaat gtcaacggat tttatattgg tgacatgggg ttgagtggaa
     9241 tggcgatcgc cgacacgatc tatgccaagt tgaccgtgga gaacattgtc aacctgacga
     9301 tcatgacctt tgtagtgacc ttactggcgg ggttgtatcc tgccatcatg gcttcccgca
     9361 tgcaaccggt tgaagccctg cgtgctgaaa aataggagca aaaatggaag tcgtaaaaat
     9421 tgagaacgtt acccgtgtat ataaaattgg gaaagtggaa acgcaggctt tgcgcggcgt
     9481 caacctttcc attggaagtg gagaatttac cgcgttggtt ggaccttctg gttcgggaaa
     9541 gaccacactt ttgcagatga tcggttgtct tgaccagcct tcaagcggac atgttttgat
     9601 cagcggcaag gatgtcacca cactcaaccg caatcaacgt gccgacatgc gccgtggaac
     9661 gatcggtttt attttccagt tcttcgcgct gatccccacc ctcaccgcgt atgaaaacgt
     9721 ggaaatgccc ctcctgctaa atggacattc gccaaaagaa cgccacgaac gcgtcatgga
     9781 tttgctcaag gcagtggacc tgaccgaacg cgccaacaat cgtcccgatc aattgagcgg
     9841 cggccaacag caaagggtgg ccattgcgcg tgcccttgcc cccaagccaa cacttattct
     9901 ggctgatgaa ccaaccgcca atctggatac ggccaacggc aaacaggtca tggatatcat
     9961 gaagaaactc aacaaggaaa caggagtgac ctttgtattt gccactcatg atccgcgtgt
    10021 catcagctat gccgaccgaa tcgtcacctt gaacgatggt ttgatagaaa aagatagtag
    10081 ttcatcctga aaaaaacgta tcgaatttta gctcggtgtg ataaaaccaa ctcaagaaaa
    10141 tttccgtcat ctaaaaacga tggcaaaaca ccagcttgga gaaatataaa aaatgagaaa
    10201 gacgctattg atcgtatttg taatccttgc cacggctttg accgcctgcg gagggcaggc
    10261 cactcctgaa gccgttccca caatttcact tgatgcccca tcgcaatcct ccgggggaag
    10321 cgcgaccgcg tctgcggttg tcgtcccggt gaccaaggtt gagttgtcgt tcccgctttc
    10381 gggcgccgtg aaaactgttg aagttgccga aggtgattca gttaccgctg gccagcctct
    10441 ggtcacgttg gatacggcca tcctcgaagc acgggtggct gaagccgaag cgaatgtcgt
    10501 cacagtgaca acccaattga catatctcaa acgcacaggc acgtcacagg aaagactcga
    10561 tgccgcgcag gccaatattg accgtgccat ggctgctgtt gagatcgcca aagcacaact
    10621 ttcacaagcc acccttgttg cgccgttcga tggcacgatc gcttctgttg aaatttcacc
    10681 cgctgagttc gccagccccg ggcagatcat cattgtgatg ggcgacctca cacacttcca
    10741 gatcgaaacc accgacttca gcgaaaagga cattccctcc gtttcaatcg gtcaggccgc
    10801 acaggtcttc attgaggcgc ttgaccagga attcactggc aaggttgttg atattgcgcg
    10861 ttcttctgaa acggtcggtg gggatgttgt tttcaaggtc accattcaac ttgatgaaca
    10921 acccgacggt ttgcgctggg gtatgagcgc cgatgtgaag atcgaagaga aataacgagg
    10981 cgagaatgaa gaagatgatt tggatgacgt tcctcgctgc gactttgatt ctcgccgctt
    11041 gtggaacgcc tgccacccca accccgcctc caattcagac cgaggagcct gcttcgccac
    11101 aaaatgattt tgtctcatct gatgcagtgg tcgcttccgc cgaggtcatc cccgcacagg
    11161 attcgcagat cagttttgtc atctccgcgc ctgtgaagga agtgcttgtt aaagaaggcg
    11221 ataccgtttc agcgggtcaa accttgatga cactcgcttc gcccgacctt gagcttgccg
    11281 tcacctctgc agagttggaa ttgaaagccg ccgaacttga attcacttac tggattcccg
    11341 cccgtcacga taggccgcca gagcgccgtc agcaggcaga agcagagctt gaacaagcca
    11401 gaaaaaaact tgaaactgcc aaagccaatt tggctcaaac atccctgctg gcaccctttg
    11461 atgccaccgt cattgatgtg aatattcagg caggtgagct tgcacaggtt gggaaggccg
    11521 taattacgct gggtgacatc gcccatatgc agattgccac gacagatctg agcgaacgcg
    11581 atattccgaa cgtgaagatc ggtcaggctg taaatgttta tatcgaggca ttagatatca
    11641 ccgtaacggg caaggtactg cacatttcac ccgtttcaga aactgttggc ggagatgttg
    11701 tataccccgt caccatttca ttggatgaac agcctgaagg catgttctgg ggtatgagcg
    11761 ctgaagtgga gatcatgatt aagtaagtgg gtgatgacgt agtttgaaga taaacttaaa
    11821 cagtacgcag taaatagcga acaggtagat tggcaggtat gtgcaatagc acaactgctg
    11881 gtttgcctgt tttcttttgt tttcgtgttt tcctgtatac ttccttcata aaggagacca
    11941 atatatgacc accattactg tcattggtgc gggaagtgca tcgtttggag aaaacacgct
    12001 ttctgcgttg ttgcgttcga aaaaattgaa tggctccacg cttcgtcttg tggatcgcaa
    12061 tgcacagagc cttgatattg tccacaggct tgcagcgcgc ctgaacaaag agtggcgttc
    12121 tggttttaac atcactgcac acacaaatca caaggacgca ttggatggta cagattttgt
    12181 tgtttctgcc attgaagttg gtccgcgtga ggaattgtgg aaggcggatt tcgatattcc
    12241 gatcaaatac ggcgtgcgac aaccttatgc agaaaacgga ggtcctggtg gtttcgcaca
    12301 taccgcccgc aatgcaatgc ctgtcttgaa cattgtccgt gatatggaac aggcttgtcc
    12361 cgatgcctgg tttatcaatt tcacgaatcc gatggtgcgt atttgtgacc tggtgaatcg
    12421 ccatagcaag atcaaagccg tcggattatg tcatcagata tttattggtt attgttttgt
    12481 ggggatggtg ctggctgaag aacttggtat tgaagttcca aaagggatca cgggaatgca
    12541 ggcggatgtc gtggagcatg acctgcggga tcatgttgtc caccaaaccc tgccgcttgt
    12601 agatttacgc gccgccggga caaaccactt tacctggatc ctgtccctgc atgataaacg
    12661 cacaggtgag gatctgtatc cgctattcag agagcgtttc aaggcttttg acccgtcttt
    12721 tgagccgctg actcgtgaag tgttcgatgc cttcgattta ttccctgttc ctggcgatac
    12781 ccatttgtgc gaatatcttc catggctcag tgaccctgtc accaaacctt gggagaagtt
    12841 caatatccgc ttgtatgatt gggacgtgcg cgccaatcta cgtgatgtca gtctggatcg
    12901 cttgaatgac atggccaatg ggacggtcgc cattgaagga ttgttgaata cagattcaga
    12961 aggtgcattg gaaatgatcg agaatattgc ttatgcaggt aatcactatc atcttgcagc
    13021 gaacttgccc aatgttgggc agatcacgaa cctgccatta ggggcaacag tggaaacacc
    13081 agttcatgta gatggggcgg ggattcatcc cgtgcatgtc ggtgcgttac ctgaagcggt
    13141 tgcagagttg tgccgccgtg agttaatggt tgcacagctt tctgttgatg cggctgtaga
    13201 gggtgattat caaaaggcat tgcaatgtct gctgttggat ccggtgatta cagatatgca
    13261 tgttgcaaag cggatccttg atgattatct gttgacctat aaggagcatc ttccgcagtt
    13321 tcatggatga gatcgtgtga tacaaaaata atttccgact atttttcaag agcaggcagt
    13381 gtgttgcctg ctcttgaatc ttaaaaacgt taaagaaatt aaatgtgaca tatgtttatt
    13441 gatgttttga tgatgaatgc atgtatatat cagtgttgat attttaagat aattgtatgt
    13501 tcaaaaacct ttacataact caatagttgc atacaataaa catataaata acaaatttga
    13561 ttcattgatt gcatattaaa ttgtgttgct aatgaattga atgatgattg aaatcataca
    13621 ttcaaataaa ttgaatcaaa aataaaaaga aggacaacaa caaaggaaat attatgtata
    13681 tctgcgtatt gacaagtgtt ccagatgaaa acgatattcc gtatgaccca tcaccataca
    13741 tgaacggata tcgctggaag caacatttgg tggaaccaaa gaacgttgaa aaacaaatcc
    13801 gcgatctgat ggatgaaggt gtcgatgttt ttatcaatct gtgtgatggt acacccgatg
    13861 atgctctttc aggcatcgca ctcgtgaacg cccttgagaa acataacgct gcttttacag
    13921 gtgccgattc gaaattcttt gatcccacgc gtgatgaaat gaagaatgcg gctcgccgtg
    13981 tatcggttcc cacccccaat tggctttttg taaatcgcgt tgaagatgcc gagaaggttg
    14041 caaagcgttt gaaatttccc atgctcgtca aaccgccgca tggatatgca agtgttggta
    14101 tccgccgtaa ttcccgcgtt gaaacgatcg agcagctgcg ggaacaactt aaacttgaaa
    14161 ttgatgaatt cggccgcgct ttgattgaag agttcattga aggacgcgaa ttcacctgtt
    14221 tgatcgctga aaatcccgat gatcccaaga agccgctcac attcaccccg gttgaattca
    14281 ttttccccaa tggtgaatcg ttcaaacact acgacatgaa atggatcgat tatgaaaaaa
    14341 tgtctgtagc ggtggtggat gagccgaaga ttcaaaagac cttgcgcgaa caaaccatgc
    14401 gtgtcttcaa ggaattgggt ggtaatggat acgcccgctg tgattatcgt atggatgcaa
    14461 atggtgacat ctacatgctg gagatcaacc ccaactgtgg cattttctac ccgccgcacg
    14521 aaccaggctc ggctgacttt tcacttctca atgacccgat caaccatacg aaattcatga
    14581 agttgatcgt ccgcaacgca cagatgcgcc aaacccgcat cgctgctgag aagatcatca
    14641 agcgtcagac ccgcaaacgc cccattgccg tcgagcagat ggcttatagc taagaataaa
    14701 aaatggacac gcaagccgtg tccatttttt attctattta tccctggatg accccggcgc
    14761 catcgctggc tttgcagaca taaatgctcg cttccaatcc tgaccgttgt ttgtaggcgg
    14821 cggaaacggc ctgagtgaac gctgcggcat tttcctcgcg gaccagtgca acagcacatc
    14881 cgccaaagcc tgcacccgtc atgcgtgcgc caaagcaatg gggttgttcc tgggcgcagg
    14941 cgacgatctg gttgagagct tcgttcgtca cttcaaaatc gtcgcgcaga ctgctgtgac
    15001 ttgcattgaa taactcgccc aatctttgga tgttgcccag tttcatcgct tcaacagctt
    15061 ccagtacgcg ctcattttcg gtgacgatat gtctggcgcg tttgagcacg ttgacattca
    15121 actcgctggc gtcttttgtt ttttcgttga attcagccac actgacatcg cgcaaggctt
    15181 ttactccaaa ccaacgcgca gcctcttcac attgatttcg ccgctcgttg taggctgaat
    15241 ctaccaggcc tcgccttgtg gaagtatcta aaatcaccac ggcaatgcct ttgggtaggg
    15301 gaggcagttg atattcaagt gtgcgacaat cgagaaacaa ggcgtggccc gcctgtgaag
    15361 ccgcacttgc catttggtcc atgatgccgc agttcacacc gacccattcg ttttctgcct
    15421 tttgtgccag agccgccatt tgtgcggcgt tccactcgaa acctgctgtt acggcaaaag
    15481 cacgtgcagc ggcaagttcc acggcagcgg atgaggaaag cccggcgccg cggggaacat
    15541 cgcctgtgat gattgcgtca aatccgtgga gttgataacc tgctttttgc aattggtcgg
    15601 caacaccttt gatgtattcc gcccagcctt tgccttttgt gagagaattt agatcgaagg
    15661 tcgaaacagc ttgcgggtcg ttgggaagcg cggtttccat atccagagaa tggatgtgaa
    15721 cctgtgaatc cattctgggt gatagcgcga tccagacagc gcggtcaatg gccatgggca
    15781 ggacgaatcc atcgttataa tcggtgtgtt cgccgatcag gttgacacgc ccgggtgccc
    15841 gtacgatgaa tgcgggtttg gcgttgaaat gggttaggaa ggattgttcg agggtttttg
    15901 tgttcatgtt tgcttttata ccatagaggc gatgtgacag aacttgaaat tcttgaaaaa
    15961 tatgagcctg tgcttcgttt tgcgaagagc gagcgttttt tcccaatggc ggtggagccg
    16021 tatttggaga agtgtcatat ttttcccggc gggccacagg gtgtgttgga gatcgtgatg
    16081 cacatccatg aaccgatgat aacgcggatt gggaaattgc agagtgaaca attctttctg
    16141 cgtttcatta ataagccgct aaatgatgcc gatgcctggg tgtggtgggg cctgctatcg
    16201 gctgctggta tggtgggtgg gtggcttact gctaattttg cgggggtgga gtttatgttt
    16261 gttgcttctt tgctgggggc attggtgttg tttatgctgg cttctccgat acgtttgcgc
    16321 attatccctg ctgtattggc ggcgggggct tttattacag cggaggtgac accgatctgg
    16381 tttttccttc atccaagcag ggttatgagt gttgcagtgg agtatttggt cctgctgccg
    16441 atctatctgg tgatcttgtt ctatctttcg atgcgtacgt tgaagtttat tcttgacagg
    16501 gtggtgcctg aaggaccagg tgtggtgatg gatatgtttt cacaagccac tgagaagatc
    16561 gcacgtgagg catattttga gtatgcggcg atgcttgcaa tggatgcgca gcctgtatat
    16621 tacggacggg tattgcatga aaaagatgaa gatggttgtg catggacgat cctgcagtat
    16681 cacttttttt atgcattcaa tgattggcgg ttggctgcaa atgggatgaa ccaccatgaa
    16741 ggggattggg agatggtggc tgtgtatttg aagaataatg aaccttatgc agttttgttt
    16801 tcacagcatg gtgcaggaaa tatcgaaaaa tggaagacgg tacgcaaggc tgttgatgca
    16861 aatggtcatg agacgacaca tccggtggtg tatgtggctt tggggtcaca tgcaaattac
    16921 agcaagccag tggtcattcg ttcaccgtgg atgtacaagc ccgggaaatt gcagcaagtc
    16981 cttttctggc tggatggttt cattcattac ctgtttttgt tgttgaatcc gaaccaaagg
    17041 gcgcgggaga ttgcgttgcg tgagttttgg aagcagggcg gaaaattcct gaccgaggaa
    17101 atgtttgccc gattgcgcga tgaaaccgac cattatattg tgaccctccc aatggagatc
    17161 gcctccgggg atgggcaccg tattggtttg agcggacagg gttcacatga gggttttata
    17221 agatccagca gatatatcaa gcgggtaatg tcggatcgca agacgacaaa gccacaggtt
    17281 gcagagtggc ggcgggtgct cttgaatcct gagccagatt gggtacaata caaaggcttg
    17341 tggggtgtga aaagtatgct cggtgaagaa tcaggcccgc ccggtcccaa gtgggatagg
    17401 ccgggaaaga agcaggtcgg tgttcaggag cgtaaacgct ggggcaggcc gttggaatgg
    17461 cttgcggagt tggaaaagac agtacctgac aggaagtaaa acatcatacc caaggagaga
    17521 aacacatgtc catttcattg gttctcgaag tagccgtcgg tctcattgtc atatactata
    17581 ttcttggttc tgtggtttca ttgatcacac aatggatcaa cgaatttttc gaatcacgcg
    17641 gcagggcttt ggagcaatac ctgatcaagc tggtcggtga taaaaagatc ggagatttga
    17701 agaatctgcc gcagcttcag tctttgcgcc cgattcggtt taagggcccg ttgagtgtgt
    17761 ttgggtcggt gaccgagccg aagatgatcg agaagatccc agttgccact cttgtagatg
    17821 cgtactttga tctcgcaggg ttgaccgcca aaaaagagat ggagttgttg gaattggctg
    17881 agttggtgga cagattgccc gattctgaag gcaagcaggc ctttattacc tggatcaatc
    17941 aaggtgtgac cagcattgca gaattacggg tgagggctac atcatatttc acaggtattt
    18001 tagagcaggc ggccgccacc ttccgtgccc gtgcccgcag ttttgtgatc attctttcga
    18061 tcggaattac cattctgttt ggcaccgata ccattcaact tgcccgtaat ttgtggacga
    18121 atgcggaatt gcgagccatt gctgctgcac aggcaaatgc tgttgtggca cgagaaggcg
    18181 ccagcgccga tctttctgac ctgatcgatg acctgagcca gtattcgatc aagatcggtt
    18241 ggtggcaaac tcagcaattc cccgaaggga acaatgtagg ggattgggcg ttgttcattt
    18301 ttctgaaggt gctgggattg ggtgtcactg ccgcggcggt ttcgcaggga tcatccttct
    18361 ggtatgacct gttgaagaag ttgacgtctc ccacttcaag ttcaagcggc agtaagagca
    18421 gcccaagtgg cggcagtagc agcggataaa agccaaaaaa attccgaggt gttgagcctc
    18481 ggaatttttt tatttcgacc atcttgcata tactgcctgt gagagcaggc ggtttgcgct
    18541 tttctcgcgg gtgaccaatt cgccgctgtc gatgttgcca ttatattttt tcatcatctc
    18601 atccattgcc tgcgccacgt ggatcgccga tgcgcgtacg gcatagacgg tgacaatgac
    18661 gaagaggggt gagtcgctca ggcaggcgcg gcacatttcc agcaagttgg gcagggattt
    18721 gtacacttcc catacctcgc ctttcggtcc gcgcccgaat ttgggcgggt cgaggatgat
    18781 gccatcatat ttcacaccgc gcttttcttc acgttggacg tatttgaccg catcttccac
    18841 gatccagcgg atcggttttt cgttcaggcc agagagtgcc tggttttcgc gtgcccagcc
    18901 caccgatttt ttggaggcat ccacatgagt cacttgcgca cccgccgccg ccgccgccaa
    18961 agatgccagc ccggtgtatc caaataggtt gagcactttt gcaggccgac ctgcctttcg
    19021 gacggaatca gccataaagt cccaatggga tgcgacttct ggaaatacgc cgaggtgacg
    19081 gccgggggtt gtcatcacag aaaaggtcag atcctgcaca tgggggagcg gatatttcat
    19141 ctcccatttt tcaggcagtt tctttttttc gatccaatga ccgccgcttt cttcggctgt
    19201 gggttggaaa acggcatgag cgttgttcca ctctgaagag gggagagcgc ggctccacat
    19261 ggcttgcgat tccgggcgcg aaaaaatgta tttgccgaag cgttccagct tttgaccgtc
    19321 gccagaatcg agcaattcat aatcacgcca gttggaggct tcgagtaggg agaaagtagg
    19381 gggtttttgc atggacatta ccaaattgtt atttaaatgg aatgaaacga acgcttcccg
    19441 ccacttgatt tttgaccaca aatcggtata atgtggtaag aagtggtaga aagtggtaag
    19501 gacgccggat tgcccggcgt tcgtcgcatt aaaaggaatt gtagcacaaa tgttcttagg
    19561 tcacttccaa cacaatctcg atgacaaagg gcgcctgatg atcccagcgc gctttcgtga
    19621 gttgttggct ggtggtgcct tcattacaca aggctttgat aaatgcctta tggtgatgac
    19681 tgaagactac ttcaaacagg tttatgaacg cattgaagcc atgaaccttg ccgacccaac
    19741 cgcgcgttta ctgcgccgtt tgatcctttc gaatgcttat cccatcgaag tggataaggt
    19801 tgggcgtatc cttgttccgc aaaacctgcg cgcttttctt ggaatcgaaa gcggcgaact
    19861 ggttgtggcg gggcagggcg aatacttcga agtgtggacc cccgcacact ggggcgagca
    19921 aatggctcaa ttgcaggata ccgaagccaa taacgcccgc ttctccacgc ttgacctttc
    19981 gaaaccagcg aactagttga ccagcgacta gttatgcagc ataaacctgt actttaccaa
    20041 gagattatac acgctctgca accccgcaac ggcgggcggt atgtagatgg cactttgggc
    20101 gcgggcggtc acgctcgcgg gatcctggag gcttgcacgc cagatgggct tttgctcggt
    20161 ctcgatgtag acccgcaggc tttggcgctc gctcgtgaga ccttggctcc ttacgaggga
    20221 cgcattcatc tcgtgcaagc ctcccacatt accctcagcc agcaactggc cgcgttgaaa
    20281 tgggactcga ttgacggtat cgtcctcgac ctcggcgcat cgtccatgca attcgataat
    20341 gccgaacgcg gattttcctt tatgcaggat ggcccgctcg atatgcgctt tggaatcaac
    20401 gccatgatga gcgcggagga gatcgtcaac accttcgatg aacgggaact ggccgatatc
    20461 atctttcgct acggtgaaga tcgcgactcg cgcaagatcg ccaaagccat cgtgatgaac
    20521 agaccgcttc gcaccacccg tgaactggtc gctgtcatcg aaaaagcatc tccccgtcac
    20581 ggtgaccgtg tccaccccgc cacgcagacc tttcaagccc tgcgcatcgc cgtcaacgac
    20641 gaattggccg cagtggagaa aacgcttccg caagccgtgg cagctctcag gtcaggtgga
    20701 agatgcgcgg tgatctcctt ccactcgctg gaaga
//
LOCUS       DAOQJC010000066        20248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1215_length_20248_cov_8.950478, whole genome shotgun sequence.
ACCESSION   DAOQJC010000066 DAOQJC010000000
VERSION     DAOQJC010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 20248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20248
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1215_length_20248_cov_8.950478"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLE39_11225"
     CDS             complement(<1..448)
                     /locus_tag="PLE39_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68946.1"
                     /translation="MTENNSKPVYRIADLHESDRPRERLVALGPQALTSAELLAILLR
                     VGVVGENAVEVGQRLLKKFGGLSGLHRAPIKELMDQHAIGEAKAAQIKAAIELGRRLT
                     LEAPEERPSINSPADAAALLQFEMSALEQEHLRVLLLDRRNRVLETV"
     gene            complement(534..689)
                     /locus_tag="PLE39_11230"
     CDS             complement(534..689)
                     /locus_tag="PLE39_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNE68947.1"
                     /translation="MLFAPKEKGQGLVEYALILVLVAIVVIAALMILGPIIGNVFSKV
                     NSSLSNV"
     gene            1005..2279
                     /gene="hutI"
                     /locus_tag="PLE39_11235"
     CDS             1005..2279
                     /gene="hutI"
                     /locus_tag="PLE39_11235"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNE68948.1"
                     /translation="MLIHSASQLLTLAGGPQRGHALGTLGIIENGAVIVRDEKIVAVG
                     TTEELKNAYPDEPTLDAGQSVLMPGFVDPHTHVIWAGDRANEFEMKMGGMAYLDILAA
                     GGGIISTVKATRTASLETLIAQTRPRLLKMFSHGTTTAEAKTGYGLQTSTELRLLKAL
                     IALDDESPLDLAITFLGAHAIAPEYKDNPQGYTDEVVNTMLPMLKEWWETHAPRLPLP
                     FVDVFCETKAFDLEQSRQILTRAGSLGFPLKIHADEFDNLGGAALAAELGAASADHLV
                     RTSDADIAALGKSDTVAVALPCTPFGLAEKDYTPARKLIEADAMLALATDCNPGTSWN
                     ESMQFAIALACRYMKLTPAQAIAAATINSAHAIRRSDSIGSIEVGKQADMLILTVPDY
                     RQVGYRYGTNLVKQVIKRGRVYSVDVGYYRTA"
     gene            2292..3908
                     /locus_tag="PLE39_11240"
     CDS             2292..3908
                     /locus_tag="PLE39_11240"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE68949.1"
                     /translation="MDIRWIIFGVILVINSIFAITTIVLITRKFDVAGRDALIMMLCS
                     LAIWSFAYAMITFSSDLETKKFWLRVENVGILSQPVFWIVFILGYSRQGRALNWGYIA
                     LLSIVPAVSMIMIASDRWFHLYYSSVKLLDGNYGPLVIERGPWYGVAVFQAYALDLIA
                     TILLIWRFIQFRNIFRTQLVFLIVAALIPLFTNIGYQVVSIFLPSLFLPVDLTPISFT
                     LSISLISSGIFRTRLLDLVPIARDVVMEHIPEMVFVVDAHDRVLDVNTVAEKWLGMTR
                     DQIIGKDPIEIFGKWPDLYNRFLTTEKTREEVKIYGDPPLTLEVVITPLYNNEKINGR
                     VVLAYDITKRKQLELDLKAKIIEIDSLRVQLQDQAIRDSLTGVFNRRFLAEALEREIA
                     KANREDMPISIVMMDVDHFKSFNDTYGHKCGDAVLRDLAGFLTKNSRHGDIVCRYGGE
                     EFVILMPEASFQNAFERAQSWQQAYASKTIEYEDKALKVTFSAGVASYPQHGPNGEAI
                     LHAADHALYESKSNGRNRVTAFQKRLTKEN"
     gene            3911..4816
                     /gene="rocF"
                     /locus_tag="PLE39_11245"
     CDS             3911..4816
                     /gene="rocF"
                     /locus_tag="PLE39_11245"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HNE68950.1"
                     /translation="MQIDIIGVPIDLGADRRGVDMGPSAIRYAHLQKKLEDLGYDVHD
                     EGNVEVPIAEMCKITNPKLKYIDCIIPMSRRVAGAVATSIQSKNFPLVLGGDHSLSIG
                     SVRGAARNKKIGVIWIDAHADFNTAETTPSGNIHGMSLAVLAGLGDKSLVQLWDENIP
                     VIDPSKIAIIGARDLDSGEKVNLSNAGAMVMGMEQIDRYGMVSVVEKAIERVSRDVDG
                     IYLSLDLDSLDPEHAPGVGTPVSGGLTQRETHLACELIAETGKLIGMDVVEVNAILDV
                     QNRTASLAVDLALSALGRRIWNGQS"
     gene            4813..5583
                     /locus_tag="PLE39_11250"
     CDS             4813..5583
                     /locus_tag="PLE39_11250"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNE68951.1"
                     /translation="MKPTLKEVEQLARGAGAILRERYNQEHEISYKGVIDLVTEADHA
                     SEAFLIGEIQSKFPGSHILAEESGETKGSNEGVWYIDPLDGTVNFAHHIPVFCVSIAY
                     AFEGSMLLAAVYDPLRDEMFTAERGKGAFLNGKPIHAAKTTELQRSLLVTGFPYDTWN
                     TEKDNFKNFEKLAKMTQGVRRLGSAALDCSYVGAGRFDGFWELTLRPWDIAAAGLIAE
                     EAGAKVTATDGKPDYISAPQSILATAPGIYQQMLEQLN"
     assembly_gap    5593..5692
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5697..6710)
                     /locus_tag="PLE39_11255"
     CDS             complement(5697..6710)
                     /locus_tag="PLE39_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68952.1"
                     /translation="MKKLTACLFIFSSLWLNACGSPGHNSNLNASILPDDCRVQAGGQ
                     LALTLDGQIPANTQIQWSASTGEVVWTGQGLTATFLAPNLPGEAVISVLFVSGTPAPY
                     TASRACAVTEQDSTASAPTVNDPAAITIAISEVMGNQCGGLETRKFNQYVELYNFGDL
                     PVDVGSWMLFDEGEKGTPDRLVAWDSRSSLQLDPTLVTNSTVIPPRSFAVVLSPLYPE
                     GNIENRMPYKFPPGVVILTAESSQTLGDDYFGIISDQDGYDTVTLYIGSDTVVDLVVD
                     TYGTPTINGPYPVNIDDNHLDHTPLHLSECESAERIDPRQPDSEANWLALPGGSPGDG
                     PYQ"
     gene            complement(6714..7367)
                     /locus_tag="PLE39_11260"
     CDS             complement(6714..7367)
                     /locus_tag="PLE39_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68953.1"
                     /translation="MSRKPLEFISGLAVYIVLTLLIAFAVILRLSVVPVQFINWLGQS
                     HNPQNALAYFSMAFSATILVFLRFGIEIPRIKMVTSSEKLQPYLSGLPLWSLGLLWAG
                     SLIAFWNVFPSCQPPVTIQFEISGREGFFQPSDRVPISPGEVVTVTAHSVNTDTTLSC
                     SWEYAGGIFETIGAQRGCQVSAKFNQQTGSGFMTVSVSENFCSQTSIFSLQPVIGTP"
     gene            7469..>8939
                     /locus_tag="PLE39_11265"
     CDS             7469..>8939
                     /locus_tag="PLE39_11265"
                     /inference="COORDINATES: protein motif:HMM:NF022517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE68954.1"
                     /translation="METKKLILVTGATGYVGGRLVPKLLEAGYHVRCLVRDPSRLQGR
                     SWLKRVEVISGDALSPEGLAAAMQGVSAAYYLIHGQQGGKIDAERDLQVARNFAQAAE
                     DANIERIIYLGELVDPTANLSPYLRARHETGYILRYGSVPVTEFRAGMIVGSGSALFE
                     MIRYLVEREPLLICPAWFFSQAQPIAIRDVLSYLVDALKTPDSNGRVIEIGGPSRLTY
                     ADMLLNYAKERSLKRWLIPTPFNAPRLSAYWVHMITPIHWRVVAPLIEGLRARLIVRD
                     DTARKLFPQIKPIDFQTAAHLALGRIQRDNVETSWADALVTSTGDIKPYEFTVEEGMF
                     IERRQILLDLKPESVFRAYSGIGGERGWLYMDWSWAIRGWMDKAIGGVGLRRGRRHPD
                     EIHTGESLDFWRVENVEKNRLMRLRAEMIVPGKAWLQFESTPQGTQEEKTLLTVTAYF
                     APHGVPGFLYWYAMWPFHKFIFDGLARQLASRARVLSHSY"
     assembly_gap    8940..9039
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9040..10340
                     /locus_tag="PLE39_11270"
     CDS             <9040..10340
                     /locus_tag="PLE39_11270"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNE68955.1"
                     /translation="TSMKPLLILVTGATGYIASRLIPQLLDLGHTVRALARRPHQIGP
                     RNWSAHIDIFPGDVTNPASLAPALKDVHTAYYLIHNMSSGHGYTDIELQAARNFARAA
                     EEAGVKHIIYLGGLADPEQHIAPHMRSRIETGATLRAGKVPVTEFRAGVIAGSGSISF
                     EMIRFMTELFPIIPAPAWIQNKSQPIAAQNVIDYLLAALENWDGHGKVFEIGGPDITT
                     YQDLMLRYAHIRGYKRSFLVLPYVPVWFMAFGIGLMTPVPRPIAHALVGGLSSDSVVM
                     HDHARAVYSKIKLIDFDSAAKEALTHLHPHRIERVWDDGTGRSKTLKHEGFFIAHHEA
                     KTNAAPEQIFTAIKNMKWQEFVFDSIEENRILLLRSKHTSVGPMFLEWRLTNQNHIAL
                     TYYLAPRGPLGFLYWHLLYPLHTYQLRKLLKRVVRNSVVK"
     regulatory      10313..10509
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            10621..11595
                     /locus_tag="PLE39_11275"
     CDS             10621..11595
                     /locus_tag="PLE39_11275"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNE68956.1"
                     /translation="MFRKILVLALLLALFITGCGPARSATPTEVPAPVSTEIPATEAP
                     SSSAEIQLTDGLGRSVVLKEAAQRIISLAPSNTEILFAIGAGSQMVGRDSLSDYPAES
                     ANVTDIGSAYEALNTEMIISLKPDLIIAAEINTPEQVKELEDLGLTVYYLNNPHTLEE
                     LYDNLQIVAQLTGREAETTALIDSLKARVAAVDEKIAPLSSRISIFYEIDGTDPAKPY
                     TAGKDTFITLLIERAGGYNIAADIEGYPQLSLEQVVAADPAFILLGDAAYGVTPESIA
                     TRPGWENLSAVKNGQVFPFDDNLLSRPGPRLVDGLEALAKLLRPELFK"
     gene            11649..12644
                     /locus_tag="PLE39_11280"
     CDS             11649..12644
                     /locus_tag="PLE39_11280"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNE68957.1"
                     /translation="MRRPFLFSFLFLLAALTLSLAVGSVFISPEELWRVISGTTTNET
                     FRTILIDIRLPRTVLIALVGAALAGSGAAYQGLFRNPLADPYLIGVASGAGLGAVLAM
                     SVKWPYTTLGLLAVPLAAFLASLLTVYLVYIFANVGGTVPTTSLILAGVAFSSFATSL
                     TSFLMLKSTNELRRAIGWLLGGVSLAGWDVILALIPYLAIGLTTLILNGYALNLLQFG
                     DDQAIQMGLNVRRVKVVIIVAASLVTAAAVSFAGIIGFVGLIVPHVVRIWWGVDYRRI
                     LPLSVVGGAGTLLLADVLARVILAPQELPLGIVTALAGAPFFLWVLRRAKNQGIW"
     gene            12664..13194
                     /gene="cobO"
                     /locus_tag="PLE39_11285"
     CDS             12664..13194
                     /gene="cobO"
                     /locus_tag="PLE39_11285"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNE68958.1"
                     /translation="MSKNTKGLLIVNTGDGKGKSTAAFGMVLRAWGRNFRICVIQFIK
                     AETGQWGEIKAAKKLGIEWHATGDGFTWTSKDMDETIARAHHGWEIAKEKIASGNYDL
                     IVLDEFTYTLAYNWLDTNEVMDWLRLNRPSNLHLAITGRSAPQVLIEAADLVTEMTPI
                     KHPYEQGIVAQAGVEF"
     gene            13196..14239
                     /gene="cobT"
                     /locus_tag="PLE39_11290"
     CDS             13196..14239
                     /gene="cobT"
                     /locus_tag="PLE39_11290"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF000996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNE68959.1"
                     /translation="MQLPLIAPLGQLAMQSASERQNRLTKPAGSLGRLEELAIQLAGV
                     TGKSNPSIKDKVIITMAGDHGVVEEGVSAYPQEVTPQMVMNFLTGGAAINVLARHIGA
                     RVVVVDMGVANEIPTRDEKLIRRRVALGTKNLAKGPAMTRSQAEESIQSGIEIALAEI
                     AKGADIIGTGDMGIGNTTPSAAIACVFLKQHPKEIAGRGTGVDDAGLARKIATIERGL
                     EVNKPNAEDGIGVLEKVGGFEIGGLAGVMIGAASKQVPVMVDGFISTAAAMIAVSLAP
                     HCHEYLISAHRSKEHGHSLMLEWLGLKPLLDFDMRLGEGTGAALGISMAEAACKILNE
                     MATFEEAGVSSKD"
     gene            14260..14997
                     /gene="cobS"
                     /locus_tag="PLE39_11295"
     CDS             14260..14997
                     /gene="cobS"
                     /locus_tag="PLE39_11295"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNE68960.1"
                     /translation="MISIIAAFQFLTIFPTLIKRMFTSQEMGRAVGWFPLVGVVLGFA
                     LFGVNYAARLLFPQTVAAALTLFAWIAFTRFFHLDGFMDTLDGLFGGWTPERRLEIMK
                     DSRMGAFGTIGGILVLLTKFAALSEIHASAMLAAILLATTLGRWASPLVIYAFPYARE
                     DGLGIEMKRNVTFREIIIATLTAGLTAWFTFGWPGFAFMLGAALIGFLAARYAMRLLP
                     GLTGDIYGTVTTLVEMLTLVAFTANLT"
     gene            15015..15623
                     /gene="cobC"
                     /locus_tag="PLE39_11300"
     CDS             15015..15623
                     /gene="cobC"
                     /locus_tag="PLE39_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase"
                     /protein_id="HNE68961.1"
                     /translation="MKLYLTRHGQTDWNTAGRYQGQSDTPLNETGLRQAEQIAKRLSK
                     ETIHAIYSSDLSRAANTAQSIADFHALEIKKDSRWRELSFGDWEGMTYQEMSASSPEL
                     FEAWMKDPLTISTPNGETLAQLAERVKAAFNEIKREHKDQTVLVVAHSGSLQSLLSVT
                     LGVDLNRYWQFRISQASLSEMNVYEDSVVLNLLNDISHFQEK"
     gene            15625..16164
                     /gene="cobU"
                     /locus_tag="PLE39_11305"
     CDS             15625..16164
                     /gene="cobU"
                     /locus_tag="PLE39_11305"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HNE68962.1"
                     /translation="MSTLTLILGGARSGKSSHALNLAKESGKPVTFIATAQAFDEEMS
                     ARIQKHKAERPAEWSTLELPMNLASHVNTIRSEVVILDCVTLLATNLMMQFVKDDLVN
                     ETPYLDAVQNETEELLTTIRNTDQNWIIVSNEVGLGLVPPYQMGRVYRDGLGWANQRL
                     AREAETVLFMVAGIPMKVK"
     gene            16198..16971
                     /locus_tag="PLE39_11310"
     CDS             16198..16971
                     /locus_tag="PLE39_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE68963.1"
                     /translation="MLKLHSLSVSYHDHRVLHDVSFDVNSGEVLALIGPNGAGKSTII
                     RAASGVIPSTGHIRTNGDDFQALSPLQRARYLAVVPQAISLPPAFTVWETVLMGRTPY
                     LGFLGHASTHDEELARQALSRVNALEFAERRVGELSGGEAQRILLARALCQSTPILLL
                     DEPTAHLDLQYQVSLLELIHELAHKENLAVMVALHDLNLAAHFADRVALLVGGHLKAI
                     GTPREVLTSELISQAYCLPVQVVEHPFLGGPLVLPEKMQ"
     gene            17094..17699
                     /locus_tag="PLE39_11315"
     CDS             17094..17699
                     /locus_tag="PLE39_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE68964.1"
                     /translation="MQTADLAIHWNPQETQPAMTQPTDDLDLIHRMQAGDDDAVRDLY
                     AQYGQRLYAYALRLTDDPATAEDVTQNTLVTAWRTAKKFRGEGRLIAWLLGIVHHTAM
                     KAIRSTPQFLGEAEETIPETLPTPEEQAQVKEEKRWVRDGLQSLSAEHRAVLDLIFYQ
                     SLSLNEAADVLKIPVGTVKSRLSYARQHLRGVLSRTEENWR"
     gene            17696..18565
                     /locus_tag="PLE39_11320"
     CDS             17696..18565
                     /locus_tag="PLE39_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNE68965.1"
                     /translation="MNHEEQIILYAANRLDEQARADFEKHLADCADCQADLLLWRSIA
                     DEINASDSLVPAPAQLAESALEAIRQPNKLARALRSTGQLLRMQMLIIHNELWVGSAA
                     LMFIFLAMALLVERVEVLYFFAPMIAAGTVTLIYGSENDPAAELTFATPISPWKVLLA
                     RLTLVSAYNVLLAGCATLALLLIIPPQALGEFILGWLAPMTFLSALALMLSLWIGTGN
                     ALFIAYLLWSAQYASLISFGEWFAFASPWLETYRQFWQNPAMLFIFGFALIVFALWSS
                     GRDQVQWRKSIAM"
     gene            18697..19473
                     /locus_tag="PLE39_11325"
     CDS             18697..19473
                     /locus_tag="PLE39_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE68966.1"
                     /translation="MSRFIGVFRHEFNMSIRRSGMWVAYGLLFVFYTVTVLWPSPEES
                     NVFTDATLWQLAGQLAYTFNLFLPVAAGILAADRMQRDFRGGVHELQSSTPLSNLVYI
                     LGKYFGVLVASLAPVLVWIWVVTACLVATGNAPINLMYAMTVTFFAMIVPAFAFVIAF
                     SLACPLIMPLRVYQILFTGYWFWGNYIPPEIFPTLNGTLLTAGGMHAYQGYFGGFHGL
                     QDPLTYTATDATLNLLVLGLCIAAVMTVLNYYLQWQSRRE"
     gene            19482..20144
                     /locus_tag="PLE39_11330"
     CDS             19482..20144
                     /locus_tag="PLE39_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68967.1"
                     /translation="MKRFSLKYTLLTYRLDQLWFPLAFLAFFVILTLLLRNEVQNYDI
                     ARAYVGFAVPLVGGFMAAYAILDDPALELRFATPIRAAQILFERTTLIFLIQAVCANA
                     FLLFCLIIGVDFSPLGNWVNVQLCWLLPTITLIALGTFGALIGAQTMVGSFLVGLVWF
                     VELIIRDGMAVNNWKYTYIFMGILAPEHPDLVSNQIALFALTIAFLFVAWALLRKQER
                     YI"
BASE COUNT         4658 a         5639 c         5072 g         4679 t
ORIGIN      
        1 ccacagtttc caagacccga ttccttctgt ccaacaaaag cacccgcaga tgttcctgct
       61 ccagcgccga catttcaaat tgcaacaatg cggcggcatc tgcgggacta ttgatggaag
      121 gccgttcttc aggggcttcc aaagtcaacc ttcttcccaa ttcaatcgcg gctttgatct
      181 gtgcggcctt ggcctcccca atggcatgtt gatccatgag ttctttgatc ggtgcgcggt
      241 gcaacccact gagcccgcca aattttttca acaaacgctg ccctacttca acggcatttt
      301 caccaaccac acccacacgc agcaaaatgg caaggagttc ggcacttgtc aacgcttgcg
      361 gccccagcgc caccaatctt tcacgcggac ggtccgattc gtgcaagtct gcaatgcgat
      421 acacaggttt cgaattgttt tctgtcatgg ccactccttg gtttggtgca tgcaatttta
      481 caacaaaaaa aagccccgtc tttcgacggg gctttttttt gaaaaggttg ggattacacg
      541 ttgctgaggc tggagttcac tttgctgaac acgttgccga tgatgggtcc gaggatcatc
      601 aaagccgcaa taacgacgat agctaccaaa acgaggatca gcgcatattc aacgaggccc
      661 tgaccttttt ctttaggggc gaataacatg gatgtatttc tcctttcata aaatatgctc
      721 aggaacgctt cctgaattac ttttatttta cccactccag aaaaaaacac aagagaaaaa
      781 cgattgcatc ttcttaacag ttatgttagt catttatact ttattgtatg ctatcctcgc
      841 aaaaagcagg acaaggacag tttcatatca tccccccgca acaaactttt ctgtggttaa
      901 attgcgcaac ataaatgagc caggtgcgaa caacttatct ccacgtgcat ggctgcggga
      961 acgtttatga aaacagccta ctattgaaag aatcggcggt catcatgtta attcattcag
     1021 catcacaact tctcaccctt gcaggcggcc cgcaacgcgg acatgccctc ggcacacttg
     1081 gcatcattga aaatggcgct gtcattgtgc gcgatgaaaa gatcgtcgca gtcggcacca
     1141 ccgaagaact caaaaatgcc taccccgatg aacccacact ggatgccggg caatcggtgc
     1201 tgatgcccgg atttgtagac ccgcacacac atgtcatttg ggcgggtgat cgagccaacg
     1261 aattcgaaat gaaaatgggc ggcatggcct acctcgatat ccttgcagca ggtggaggca
     1321 tcatctccac cgttaaagcc acacgtaccg ccagccttga aacgctcatc gcacaaaccc
     1381 gtccacgcct gttgaaaatg ttctcgcatg gcaccaccac cgccgaagcg aaaaccgggt
     1441 acggtctgca aacttccact gaattgcgcc tgctcaaagc actgatcgcg cttgacgatg
     1501 aaagtccgct cgaccttgcc atcaccttcc ttggcgcgca cgccattgcc cccgaataca
     1561 aagacaaccc gcaagggtac accgatgaag tcgtcaacac catgctgccg atgttaaagg
     1621 aatggtggga aacacatgcc ccccgcctgc ccctgccttt tgtggatgtc ttctgcgaaa
     1681 ccaaagcctt cgaccttgaa cagtcgcgtc agatcttaac ccgggcaggg tctttgggat
     1741 tcccgctcaa gatccacgcc gacgaattcg acaacctcgg cggcgcagcg ctcgccgcag
     1801 agttgggtgc cgcctctgcc gaccatctcg tacgcacatc cgatgcagat atcgccgccc
     1861 tcggcaaatc agatacggtg gctgtagccc tgccatgcac accctttggt ttggcagaaa
     1921 aagattacac ccccgccaga aaactcatcg aagccgatgc catgctggct ctggccactg
     1981 attgcaaccc aggcacctcg tggaacgagt ccatgcaatt tgcgattgca ctggcctgcc
     2041 gctacatgaa attgaccccc gcacaagcca tcgccgccgc cactattaat tccgcccacg
     2101 ccatccgcag gtccgactcg atcggctcca tcgaggtggg caagcaagcc gatatgctca
     2161 tcttaaccgt gccggattat cgccaagtgg gatatcgcta cggcacaaac cttgtcaaac
     2221 aagtcatcaa gcgcggacgt gtctattcgg tggatgttgg ttactacaga accgcataac
     2281 gaaggaaatt tatggatatc cgttggatca tttttggggt cattctagtc atcaattcga
     2341 tcttcgccat cacgacgatc gtcctcatca caaggaaatt tgatgtcgcc gggcgcgatg
     2401 cattaatcat gatgctttgc tccctggcga tctggtcttt tgcctatgca atgatcacct
     2461 tctccagcga tctggagacc aagaagttct ggctgcgggt ggagaatgtc ggaattcttt
     2521 cacagcctgt tttttggatc gttttcattc tggggtattc acgacaggga cgtgccttga
     2581 attggggtta tattgccctg ctatccattg tccccgcagt atccatgatc atgatcgcct
     2641 ccgaccgctg gttccatctc tattactcat cggtgaaatt gcttgatgga aattacggcc
     2701 cactcgtcat cgagcgcgga ccctggtatg gggttgcggt ttttcaggcc tatgcacttg
     2761 acctgatcgc caccatcctg ctcatctggc ggtttattca attcagaaat atctttcgca
     2821 cacaacttgt tttcctcatt gtggcggcct tgattcccct gttcacaaac atcggctatc
     2881 aggttgtatc cattttttta ccgtcgttat tcctcccagt ggatctgacc ccgatttcat
     2941 ttaccctcag catcagcctg atcagttcag gcattttccg tacccgcctg ctcgatctcg
     3001 tcccaattgc gcgcgatgtg gtcatggagc acattcctga aatggtgttc gttgtagatg
     3061 ctcacgatcg tgtgctggat gtaaacaccg tggctgaaaa atggctgggg atgaccagag
     3121 accagatcat tggcaaagac cccatcgaaa ttttcgggaa atggcccgat ctgtacaacc
     3181 gttttctcac cacagaaaag actcgtgaag aagtcaaaat ttatggcgac ccgcctttga
     3241 cccttgaagt ggtcattacc ccgttgtata acaacgaaaa aattaacggg cgtgtcgttc
     3301 ttgcctacga catcaccaag cgcaaacaac tcgaactcga cttgaaggca aagatcattg
     3361 agatcgattc gctcagggtt cagcttcaag accaggccat ccgcgactcg ttgaccggtg
     3421 tgttcaatcg ccgttttctt gcggaagcgc ttgaaaggga gatcgcaaag gccaaccgtg
     3481 aagacatgcc catctccatc gtcatgatgg atgtggatca tttcaagtca ttcaacgata
     3541 cctatggaca taaatgcggt gatgccgtat tgcgcgacct ggcgggcttc ctgaccaaaa
     3601 actcgcggca tggagatatt gtctgccgat atggcggcga ggaatttgtc attctcatgc
     3661 cggaagcctc ttttcaaaac gcattcgaac gggcgcaaag ctggcagcag gcctatgcaa
     3721 gtaaaaccat tgaatatgaa gataaagccc tgaaagtaac cttctctgcc ggtgtagcaa
     3781 gttatccgca gcacggaccc aatggtgaag ccatcctcca tgctgccgac catgccttgt
     3841 atgaatccaa aagcaatggc cgcaaccgtg tcactgcttt tcaaaaacgc cttacaaagg
     3901 agaattaacc atgcaaatcg acattatcgg tgtacctatt gacctcggcg cagaccgtcg
     3961 cggtgtggat atgggcccca gcgcaatccg ctatgcacac ctgcaaaaga aactggaaga
     4021 tcttggctat gacgtccacg atgaaggcaa cgtggaagtt cccatcgcag aaatgtgcaa
     4081 gatcaccaac ccgaaattga aatatattga ttgcatcatc cccatgtcac gccgtgtggc
     4141 gggcgcagtg gcaacctcca tccagtccaa aaattttcca ctggtattgg gcggcgacca
     4201 cagcctctcc attggttcag tccgcggcgc tgcacgcaac aaaaagatcg gcgtcatctg
     4261 gatagacgcc cacgccgatt tcaacaccgc cgaaaccaca ccctcaggca acatccacgg
     4321 catgtcgctt gcagttcttg ccgggctggg tgataaaagc cttgtgcaat tatgggatga
     4381 aaacatccca gtcattgacc catcgaagat cgccatcatc ggcgcacgtg accttgactc
     4441 gggtgaaaag gtcaacttga gtaatgcagg cgccatggta atgggcatgg aacagatcga
     4501 ccgctatgga atggtctcgg tggtggaaaa ggccatcgaa cgagtcagcc gtgatgtgga
     4561 tgggatctac ctctccctcg acctcgattc gctcgacccg gaacatgctc ccggtgtagg
     4621 gactcccgtc tcaggggggc tgacccagcg cgaaacccac ctcgcctgcg aattgatcgc
     4681 cgaaacaggc aaactcatcg gcatggatgt ggtggaagtc aacgccattc tcgatgtgca
     4741 aaatcgaact gcctccctcg ccgtagacct ggccctctct gcactcggcc gccgcatttg
     4801 gaatggacaa tcatgaagcc aacgctgaaa gaagtcgaac aactggctcg gggcgcaggt
     4861 gccattctgc gtgaacgcta caaccaggaa catgagatca gctataaagg cgtgatcgat
     4921 ctcgtcaccg aagccgacca cgcctcggaa gcctttttga tcggcgagat ccaatccaaa
     4981 tttccaggca gccatatcct tgctgaggaa agcggcgaaa ccaaaggcag caatgaaggc
     5041 gtctggtaca ttgacccctt ggacgggacc gtgaattttg cacatcacat tcctgtattt
     5101 tgcgtttcga ttgcgtatgc ctttgaaggc agcatgctgc ttgcagccgt gtacgaccca
     5161 ctacgcgatg aaatgttcac tgccgaacgc ggcaagggtg ccttcctcaa cggaaagccg
     5221 atccatgccg ccaaaacgac cgaactgcaa agaagcctgc tagtaacagg gtttccatac
     5281 gatacgtgga atactgaaaa agataacttc aaaaatttcg agaaacttgc caagatgacc
     5341 caaggtgtgc gccgactcgg ttctgccgcc ctcgattgct catacgtcgg tgctggccgt
     5401 ttcgacggct tttgggaact caccctcagg ccgtgggata tcgccgccgc tggtctcatt
     5461 gccgaggagg caggagcaaa agtcacagca acagatggca agccagatta tatttccgca
     5521 ccacaatcca ttttggcgac cgcgccgggg atttatcagc aaatgcttga acagttgaat
     5581 taagagaaat tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgaattat
     5701 tgatacggac catcgccagg agacccgccg ggtaacgcca gccaattggc ttcagaatct
     5761 ggttggcggg ggtcaatacg ttccgcggat tcacactcac tgagatggag cggtgtgtgg
     5821 tcaaggtgat tatcgtcaat gttgaccggg taggggccat tgatggtggg cgtaccgtag
     5881 gtatccacta caaggtccac gacggtgtca ctgccaatgt aaagtgtgac cgtgtcgtaa
     5941 ccatcctgat cgctgatgat gccaaaataa tcatcgccga gggtctggct tgattcggcg
     6001 gttaggatga ccacaccggg cggaaattta tacggcatcc gattttcgat attgccttcg
     6061 ggatacagag gggaaagaac aaccgcaaag ctgcggggcg ggatcaccgt cgagttggtg
     6121 acaagtgtcg ggtcgagttg aagcgatgat cttgaatccc atgcaacgag tcggtcagga
     6181 gtcccttttt cgccttcgtc gaacagcatc caactgccga catccacagg cagatcgccg
     6241 aagttgtaaa gctccacata ttgattgaac ttacgtgttt cgagtccgcc gcattgattc
     6301 cccatcactt cgctaatggc aatggtgatt gccgcagggt cgttgacagt gggcgcgctg
     6361 gcagtggagt cttgttcggt gacggcacag gcgcggctgg cagtgtaagg ggcgggagtg
     6421 cctgaaacga aaagaaccga gatcacagct tcaccgggca agttcggggc gagaaaagtg
     6481 gcggtcaacc cctgccccgt ccaaaccact tcacctgttg aagcactcca ttgaatttga
     6541 gtgttcgcag gaatctgtcc atcaagggta agtgccagtt gaccgcccgc ctgcacccga
     6601 cagtcatcgg gcagaatgga tgcgttgaga ttggagttat gccccggcga accgcaggcg
     6661 ttcagccaca aggaagaaaa aatgaaaagg catgcggtga gttttttcat agatcaggga
     6721 gttccaatga ctggctgcaa tgagaagatg gatgtttgtg aacagaaatt ttcagaaacg
     6781 ctgacggtca taaacccact gccggtctgc tggttgaatt ttgcactgac ctgacagccg
     6841 cgctgtgcgc cgatggtttc gaagatgccc ccggcatatt cccacgaaca agacagggtg
     6901 gtatctgtat tgacggaatg tgcggtgact gtgacaacct caccgggtga aatgggaaca
     6961 cggtcggatg gttgaaaaaa accttctctg cctgaaattt caaattgaat ggtcacgggc
     7021 ggctggcagg atgggaacac gttccaaaag gcgatcaatg accccgccca taacaatccc
     7081 agcgaccaca gcggcaaccc tgaaagataa ggctggagct tttcagaaga tgtgaccatt
     7141 ttgatgcgtg gaatttcaat gccgaaccgc agaaaaacaa ggatcgtagc gctgaatgcc
     7201 atgctgaaat aagccagcgc attttgcgga ttgtggcttt gcccgagcca gttaatgaac
     7261 tgtacaggca ccaccgaaag ccgcagaatg acagcaaacg cgatgagcag ggtcaacaca
     7321 atgtacaccg ccagaccgga aatgaactca aggggttttc tgctcatgtt tctccacgcg
     7381 ttgagattat agtttttccg catttgggtc aactttccaa atcctttaca gttggcattc
     7441 cgggcggctg gcgtataata aaagttacat ggaaaccaaa aaacttatcc tcgttacagg
     7501 tgccacaggc tatgtaggcg gaagactggt acccaaactg cttgaagcgg gttatcacgt
     7561 gcgctgcctt gtacgtgacc catcccgttt gcagggacgt tcctggctga aaagagtgga
     7621 agtcatttcc ggagatgcac tttcacccga agggcttgcc gcagccatgc agggcgtttc
     7681 agcagcgtat tacctgatcc acgggcagca gggtggaaag atcgatgcgg aacgtgattt
     7741 gcaagtggct cgtaatttcg cccaagccgc tgaagatgcg aacattgaac gcattattta
     7801 tctgggtgaa ttggtggacc cgaccgccaa cctctctccc tatttgcgtg cccgccatga
     7861 aacaggctac attctgcgct atggcagtgt gcccgtcacc gaatttcgcg caggcatgat
     7921 cgtcggttcg ggaagtgccc tttttgaaat gatccgctac ctcgtcgagc gtgaaccgtt
     7981 gttgatctgt cccgcatggt ttttttcaca ggcacaaccc atcgcgatcc gtgatgtgct
     8041 gtcgtatctg gtggatgcgc tcaaaacgcc cgacagcaac ggccgcgtga tcgaaattgg
     8101 cggccccagc cgactcacct atgccgatat gcttttgaat tatgcaaaag aacgcagcct
     8161 gaaacgctgg ttgatcccca caccattcaa cgcgccgcgg ctttcggcgt actgggttca
     8221 catgatcacg ccgatccatt ggcgcgtggt ggcaccgctc attgagggtt tgcgcgcacg
     8281 gctgatcgtc cgcgatgata cggcaagaaa actttttccg cagatcaagc cgatcgattt
     8341 tcaaaccgcc gcacatctgg cgcttggacg cattcaacgc gataacgtgg aaacaagctg
     8401 ggctgatgca ctggtcacct ccacgggcga catcaaaccg tatgaattca ctgttgaaga
     8461 aggcatgttc atcgaacgcc gacagatcct gcttgacctc aaacccgagt cggtctttcg
     8521 cgcctactcc ggaatcggcg gcgaacgcgg ctggctttat atggattggt catgggccat
     8581 ccgtggttgg atggataaag ccatcggcgg tgtgggtctg cgccgcggcc gccgccaccc
     8641 cgatgaaatt cacaccggcg aatcgcttga tttctggcgc gtggaaaacg tggaaaagaa
     8701 tcggctcatg cgcctgcgcg ctgaaatgat cgtccccggc aaagcctggc tgcaattcga
     8761 atccaccccg cagggaacac aggaagagaa aaccctgctc acggtcacag cgtattttgc
     8821 accgcacggg gttccgggct ttttgtattg gtacgccatg tggcccttcc acaaattcat
     8881 cttcgacggc cttgcccgtc aactcgcctc ccgggcacgg gtgttgagtc attcgtattn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna cacctcgatg aagccacttt
     9061 tgatccttgt caccggcgca actggataca ttgccagcag attgattccc caactgctgg
     9121 atcttggcca tacggtccgc gcactggcaa ggcgcccgca tcaaatcgga cctcgaaact
     9181 ggtctgccca cattgacatt ttccccggcg atgtgaccaa ccccgcctcc cttgcacccg
     9241 cattgaagga cgtccacacg gcttactacc tcattcacaa catgtcttcg gggcatggct
     9301 ataccgatat tgagttacaa gccgcacgca attttgccag ggctgccgaa gaagcggggg
     9361 tcaaacacat catctacctt ggcggtcttg ccgaccccga acaacacatt gcgccgcaca
     9421 tgcgttcacg cattgaaaca ggcgccaccc tgcgcgcagg gaaagtaccc gtcaccgaat
     9481 ttcgagcagg tgtgatcgca ggttcaggga gcatttcctt tgaaatgatc cgcttcatga
     9541 ccgaattgtt tcccatcatc cccgcccctg cctggatcca aaacaaatcc caacccatcg
     9601 cagcccaaaa cgtgatcgat tatctgctcg ctgccctcga aaactgggac ggtcatggca
     9661 aggtcttcga gatcggcggc cccgacatca caacctatca agacctcatg ctgcgttatg
     9721 cccacatccg cggatataaa cgcagtttcc tcgttctgcc ctacgtccct gtctggttta
     9781 tggcttttgg catcgggctg atgacccccg ttccgcgccc gattgcccat gcactggtcg
     9841 gcgggctatc cagtgacagt gttgtcatgc acgatcacgc ccgcgcggtt tactcaaaaa
     9901 tcaaactgat tgatttcgat tccgccgcca aagaagcgct gacccatctg catccgcacc
     9961 gcatcgaacg tgtgtgggat gacggcacag gccgctccaa aacgctcaag cacgaaggtt
    10021 ttttcatcgc ccaccacgag gccaaaacga acgccgcacc tgaacagatc ttcactgcca
    10081 tcaaaaacat gaaatggcag gaattcgtct ttgattccat cgaagagaat cgcatccttc
    10141 tgttgcggtc gaaacataca tcggttggcc ccatgttcct cgaatggcgg ctgacgaatc
    10201 aaaatcacat cgccctgacc tattacctgg ccccacgcgg cccgcttgga tttttatact
    10261 ggcatttgtt gtatccgctc cacacctacc aactgcgcaa gttgttaaaa cgcgtcgtcc
    10321 gcaactcagt ggtaaaatga aaacataaat aacgatcggc aagcggcgaa gctggtgaaa
    10381 ttccagcact gtcgcgcaac tgtgaagtta gtcatgttgt ctctttgcct tctcgtctag
    10441 tagacaagaa gaccagcaga cgaaaagact aatgaagtca ggtcgcccgc tactgatcgg
    10501 ttcaccacac tctcgcgaaa aggaggtgga tgacggtagc ctgatcggat tgcctctccc
    10561 ctccccagcc catgctgggg attttgattc ccgtggtaac aaaaatctca aggagaaaaa
    10621 atgtttcgaa aaattttagt tctggcttta ctactcgcgc tcttcatcac aggatgcggc
    10681 cccgcacgct ccgcgacacc gaccgaggtg cctgctcctg tttcaacaga aattcccgcc
    10741 actgaagcac cctcttcgtc agcggagatt caactgacag atgggctggg caggtctgtg
    10801 gtcctgaaag aagcggcgca gcgcatcatc tcgctggcac cgtcaaatac cgaaatcctc
    10861 ttcgccatcg gcgctggttc acaaatggtt ggccgcgatt cgctttccga ttatcccgcc
    10921 gaatcagcca acgtaaccga tattggttcg gcatacgaag ccttgaacac agaaatgatc
    10981 atctcgctca agcctgatct gatcatcgcc gccgagatca acacacccga acaggtaaaa
    11041 gaactcgaag acctcgggct gaccgtgtat tacttgaaca acccccacac gctggaggaa
    11101 ctgtacgaca atcttcagat tgtcgcacag ttaaccgggc gcgaagcaga aaccaccgca
    11161 ctgatcgatt ctctcaaagc gcgcgttgcc gctgtggatg aaaagattgc gccgctcagt
    11221 tcacgcatca gcatttttta tgaaattgat ggcaccgacc ctgccaaacc ctacaccgca
    11281 ggcaaggaca ctttcatcac cctgttgatc gagcgcgcag gcggatacaa catcgctgca
    11341 gatatcgaag gctacccaca attgagtctc gaacaagtcg tcgccgcgga ccctgccttt
    11401 attttgctgg gcgatgcagc ctatggcgtt acccccgaat caattgccac ccgcccaggc
    11461 tgggagaatt tatccgcagt gaaaaatgga caggtcttcc ccttcgacga taacctgctc
    11521 agccgccccg gtccgcgttt ggtggatggg cttgaagcct tggcaaaact gttgaggcct
    11581 gagttattta agtagaacgt aaactggtaa ttggtaattg ctttttacca attaccaacc
    11641 tcccatccat gcgccgtccg tttcttttct cttttctctt ccttctagcc gccctgaccc
    11701 tcagtcttgc ggttggttct gttttcattt ccccggaaga gttatggcgt gtcatcagcg
    11761 gcacgaccac gaacgaaacc tttcgcacca ttctgatcga tattcgtctg ccgcgcacgg
    11821 ttttgattgc gctggtcggc gcggctctgg caggcagcgg cgcggcatat cagggactgt
    11881 tccgcaaccc gctggctgac ccgtatttga tcggcgtggc ttcaggtgca gggctgggcg
    11941 cggtgttggc gatgtcggtc aaatggccgt acaccacgct tggattactg gccgttccgc
    12001 ttgcggcatt tctcgccagc ctgctgacgg tgtacctcgt ctatattttt gcgaatgtgg
    12061 gcggcacggt gccaactaca agtttgatcc tcgcgggggt ggcgttctca tcctttgcca
    12121 catcactgac ctcatttttg atgctgaagt caaccaatga actgcgccgt gccatcggct
    12181 ggttgttggg cggggtgagt ttggcaggct gggatgtgat cctcgcgctc atcccctatc
    12241 tcgccatcgg cttgaccaca ttgatcttga acgggtacgc gttgaatctt ttgcaattcg
    12301 gcgacgacca ggccatacag atgggtttga atgtgcgccg tgtgaaagtc gtcatcatcg
    12361 tggcggcatc gctggtcaca gcggcggcgg tttcgtttgc gggcatcatc ggctttgttg
    12421 ggctgatcgt tccgcatgtg gtgagaatct ggtggggcgt ggattatcgc cgcatccttc
    12481 cgctcagtgt cgtgggcggt gcaggcacct tactgctggc cgatgtgctg gcacgggtga
    12541 ttcttgcgcc gcaggaattg ccgctgggca ttgtcacagc attggcaggc gcacctttct
    12601 ttttatgggt gttgcgccgc gcaaaaaatc aaggcatctg gtaacacttc atcaaggaat
    12661 catatgagca aaaacacaaa aggattattg atcgtcaaca caggcgacgg caagggcaaa
    12721 tccactgccg ccttcggcat ggtgctgcgt gcatggggac gcaactttcg catttgcgtc
    12781 atccaattca ttaaagccga gacggggcaa tggggcgaga tcaaagccgc gaaaaaactg
    12841 ggcatcgaat ggcatgccac aggcgacggt tttacatgga catcaaagga tatggatgaa
    12901 accatcgcgc gcgcccatca tggctgggag atcgccaaag aaaagatcgc cagcggaaat
    12961 tatgacctga tcgtgctgga tgaatttacc tacaccctcg cctacaactg gctcgacaca
    13021 aacgaagtga tggactggct gcgcctcaac cgaccctcga acctgcatct ggccatcaca
    13081 ggcaggtcag cgccgcaggt tttgatcgaa gccgccgacc ttgtcaccga gatgaccccg
    13141 atcaagcatc catacgaaca gggcattgtt gcccaggcag gagttgaatt ctaaaatgca
    13201 gcttcctctc attgccccac tcggacagct tgcaatgcaa tctgcgagcg aacgccagaa
    13261 cagactcacc aaacccgcag gcagtttggg acggctggaa gaattggcca ttcaactggc
    13321 tggtgtcact ggcaagtcaa atccatccat caaagacaag gtcatcatca caatggctgg
    13381 cgatcatggc gttgttgaag aaggtgtcag cgcgtaccca caggaggtga ccccgcaaat
    13441 ggtgatgaat ttcctcacgg gcggcgcagc gatcaatgtg ctggcaaggc atatcggtgc
    13501 gcgggtcgtg gtggtggata tgggtgttgc aaacgagatc ccaaccaggg atgaaaaact
    13561 gattcggcgc cgagtggctt tgggaacgaa gaatctggcc aagggtcctg caatgactcg
    13621 ttcacaggcg gaagaatcca ttcaaagcgg aatcgaaatc gctttggcag agatcgcaaa
    13681 aggcgcagac atcatcggca caggtgacat gggcattggc aataccacgc cttcggcagc
    13741 cattgcatgc gtctttttga aacaacatcc gaaagagatt gcaggccgcg gcacaggcgt
    13801 ggacgatgca gggcttgcca gaaaaattgc gacgatcgag cgcgggctgg aagtaaacaa
    13861 gcccaacgca gaggatggca ttggcgtgtt ggaaaaagtg ggcggttttg agatcggcgg
    13921 gctggcaggg gtgatgatcg gtgccgcttc gaaacaggtg cctgtgatgg tggatgggtt
    13981 tatctccact gccgccgcaa tgatcgcggt ttcgcttgcg ccgcattgcc acgagtattt
    14041 gatctctgcc catcgttcaa aggaacacgg acattcgctc atgttggaat ggcttggttt
    14101 gaaaccgctg ctcgatttcg atatgcgtct cggtgaaggc actggcgcag cgcttggcat
    14161 ttcaatggca gaagctgcat gcaagatact gaacgaaatg gccacctttg aagaagcagg
    14221 tgttagcagt aaagattaac cactcgtggt gaaagtttta tgatctcaat tattgccgct
    14281 tttcaattcc ttaccatctt ccccaccctc atcaagcgca tgttcacctc gcaagagatg
    14341 ggacgtgccg tgggctggtt tccgcttgtg ggcgtggtgc tgggatttgc cttattcggt
    14401 gtgaattacg cggcacggct gctttttccg caaaccgtcg cggctgcatt gaccctgttc
    14461 gcatggatcg cgttcacgcg tttctttcac cttgatggtt tcatggatac cctcgatggt
    14521 ttgttcggcg gctggacccc cgaacgcaga ctcgagatca tgaaagattc acgcatgggc
    14581 gcctttggaa cgatcggcgg cattttggtt ttgttgacca aatttgcggc gctttccgaa
    14641 atccatgcct cggccatgtt ggctgcgatc ctcctcgcca ctacgttggg acgctgggct
    14701 tcaccgcttg tcatttacgc ctttccctac gcgcgcgaag acgggctggg aattgagatg
    14761 aaacgcaatg tgactttccg tgagatcatc attgccacac tgacagcagg gctcactgca
    14821 tggttcacgt ttggctggcc tgggtttgcc ttcatgctcg gcgcggcact gatcggcttc
    14881 ctcgccgccc gctatgccat gcgtctgctc cccgggctga cgggcgatat ctacggcaca
    14941 gtcaccacgc tggtcgaaat gctgaccctc gttgcattta ctgccaacct cacctgaaac
    15001 ctggaacctg aaacttgaaa ctctatctca cccgccacgg acaaacagac tggaacaccg
    15061 caggccgtta tcaggggcag agtgatacgc cactgaacga gactggtctg cgtcaggctg
    15121 aacagatcgc aaaacggctc tcaaaagaaa ccatccacgc catttactcc agtgaccttt
    15181 cacgtgccgc aaacactgcc caatccatcg cagattttca cgcactggaa atcaaaaagg
    15241 attcccgctg gcgcgaactt tccttcggcg attgggaagg catgacctat caggaaatgt
    15301 cggcatcttc gcccgaattg ttcgaagcat ggatgaaaga cccactcaca atttccacgc
    15361 caaacggcga aacccttgcc caactggcag agcgggtgaa ggcggccttc aatgaaatca
    15421 agcgcgaaca caaagaccag accgttctgg tcgtggcgca cagcggatca ttacaatccc
    15481 tgctgtcggt cacccttggc gtggacttga accgctactg gcaattccgc atttcgcagg
    15541 catcgctctc tgaaatgaat gtatatgaag atagtgttgt actgaactta ctcaatgaca
    15601 tatcgcattt tcaggaaaaa taacatgagc accctcaccc tcatcctcgg cggagcacgc
    15661 agcgggaagt catcgcatgc gctgaacctg gcaaaagaat cgggaaagcc cgtcactttc
    15721 attgccaccg cacaggcatt tgacgaggaa atgtcagcac ggattcaaaa gcacaaggcg
    15781 gagcgccccg ccgaatggtc cacgcttgaa ctgccgatga atctcgccag ccatgtgaac
    15841 acgatccgct cggaagttgt catcctcgat tgtgtaacct tgctggcgac caacctgatg
    15901 atgcaatttg tcaaagatga tctggtgaac gaaacgccct atctggacgc cgttcaaaat
    15961 gaaaccgaag aactgcttac gaccatccga aacaccgacc aaaactggat catcgtttca
    16021 aatgaagtcg gccttgggct tgtcccaccc taccaaatgg ggcgcgttta ccgcgacgga
    16081 ctcggctggg ctaatcaacg cctcgcccgg gaagccgaaa cagtgctctt catggtggca
    16141 ggcattccca tgaaggtcaa ataacccatt ttccactttc atccatttcg agttcccatg
    16201 ctaaaactcc actccctctc cgtttcctac catgaccacc gcgttctgca cgacgtttct
    16261 ttcgatgtga acagcggtga agtgctcgcg ctcatcggac cgaacggcgc gggcaaatcc
    16321 accatcatcc gtgcagcgag cggagtgatt ccctccacgg ggcacatccg caccaatggc
    16381 gatgacttcc aagccctctc ccctcttcaa cgcgcacgct atcttgccgt ggttccgcag
    16441 gcgatctccc tgccgcccgc ctttacagtt tgggaaacgg ttttgatggg acgcacaccc
    16501 tatctcggat tcctcggcca cgcttccaca catgacgagg aactggcacg gcaagccctg
    16561 agcagagtca acgccttgga atttgccgag cgccgcgtcg gagaattatc aggcggcgaa
    16621 gcccagcgga ttctgctggc acgtgccctg tgccaatcca caccgatcct tttgctcgat
    16681 gaacccaccg cccatctgga tctgcaatat caagtcagtt tgttggaatt gatccacgaa
    16741 ctcgcccaca aggaaaacct cgccgtgatg gtggccctgc acgacctcaa ccttgccgcc
    16801 catttcgccg accgcgtagc cctgctcgtc ggcggtcacc tcaaagccat tggcacaccc
    16861 cgcgaagtac tgacctccga actgatctcc caagcctatt gcctccccgt tcaagtggtg
    16921 gagcatccat ttttaggcgg gccgctggtg ctgcctgaaa aaatgcaata acctgcaaca
    16981 ctgcctgcaa aagatgacat ttccaaaatg gaatgtcatc tttttttatt tcgcagcacc
    17041 aattttgaac cttttcgcgt ctcgctggta cataccattg agaagcagat acaatgcaaa
    17101 cagcagacct tgccattcac tggaacccgc aagagacgca gcccgccatg acgcaaccca
    17161 ccgacgacct cgacctgatc catcgcatgc aagctggcga tgatgatgcc gtgcgcgacc
    17221 tgtacgcgca gtatggtcag agactctatg cgtacgcgct gcgcctcacg gatgatcccg
    17281 ccaccgccga agatgtgaca cagaacacac ttgtcaccgc atggcgcacc gcaaaaaaat
    17341 ttcgcggcga gggccgcctc attgcatggc tgttgggcat cgtccatcac acggcgatga
    17401 aggccattcg cagcacgcct caatttttag gtgaggcaga agaaaccatc cctgagactc
    17461 tgcccacgcc cgaggagcag gctcaagtta aagaagagaa gcgttgggtt cgtgatggct
    17521 tgcaaagcct gtcggctgaa catcgcgctg tgcttgacct catcttctat caaagtctgt
    17581 cgctcaacga agcggcggat gttttgaaaa ttccagttgg cacggtcaag agtcgtttga
    17641 gttatgcgcg tcagcatttg cgcggcgtac tcagccgcac cgaggagaat tggcgatgaa
    17701 ccacgaagaa caaattattt tatacgccgc gaatcgactc gatgaacagg cgcgcgccga
    17761 ttttgaaaaa catctcgcgg actgtgcgga ctgtcaagcg gatctgcttc tctggcgatc
    17821 tatcgcagat gaaatcaacg cctcagactc actcgtgcct gctcctgctc aattggcaga
    17881 atctgcgctg gaagcaattc gccaaccgaa caaactcgcg cgcgcattgc gcagcacggg
    17941 acagttattg cggatgcaaa tgctcatcat ccacaacgag ttgtgggtcg gctccgctgc
    18001 gttgatgttc atctttttgg cgatggcgtt gttggtggaa cgcgtggaag tgctgtactt
    18061 ctttgcgccg atgatcgccg ctgggacagt gacgctgatt tacggctcgg agaacgaccc
    18121 cgccgccgag ttgacctttg ccacccccat ctcaccatgg aaggttttgc tggcgcggct
    18181 cactctagtc tctgcgtaca acgtgttgtt ggctggctgt gcaacacttg ccctgcttct
    18241 catcatcccg ccgcaagctt tgggagaatt catcctcggc tggcttgcac ctatgacctt
    18301 cctctccgcg ctcgcgctca tgctctcgct gtggatcggc actggcaacg ccttgttcat
    18361 tgcgtacctg ttgtggtcgg cgcaatatgc ttctctcatt tcatttggcg aatggttcgc
    18421 gtttgcttct ccgtggctcg aaacctatcg ccagttctgg caaaaccctg ccatgctttt
    18481 cattttcggt tttgcgctca tcgtgttcgc actctggtca tcgggccgcg atcaagtgca
    18541 gtggcgtaaa tcgatagcaa tgtaacaaaa ctttccctcc gtcattgcga ggggcggtgt
    18601 tttccgcccg aagcaatccc atgattaagt tggagattgc ttcgggcaaa gaacaagagc
    18661 gccctcgcaa tgacgaaata gaatttggag aaacccatgt ctcgttttat cggcgtcttt
    18721 cgccatgaat tcaatatgtc cattcgccgc tcaggcatgt gggtcgctta tggattattg
    18781 ttcgtgttct acaccgtcac cgtcctttgg ccttcgcctg aagaaagtaa cgttttcacc
    18841 gatgcaaccc tctggcagct cgcaggccaa ctagcgtaca ccttcaattt attcctgccc
    18901 gtcgccgcag gcatcctcgc cgcggatcga atgcagcgtg actttcgagg aggcgtgcat
    18961 gagttacagt ccagcacgcc gctcagcaat ttggtgtata tccttgggaa atatttcggt
    19021 gtgcttgtgg cttcccttgc tcctgtcctc gtttggatct gggttgtgac agcctgtctc
    19081 gtagccactg gaaatgctcc catcaatttg atgtacgcca tgacggttac attctttgca
    19141 atgatcgtcc ccgcttttgc attcgtgatt gccttctcgc tggcctgtcc actcatcatg
    19201 cccttgcgcg tgtatcagat cctgttcaca ggctattggt tctggggcaa ttacatcccg
    19261 cccgagattt tcccaaccct caatgggaca ttgctcaccg caggcggaat gcacgcctat
    19321 caaggttact tcggcggctt tcatggtttg caagacccgc tgacttacac cgccacagat
    19381 gcaaccctca acttgcttgt gcttggcctg tgcattgccg ctgtcatgac cgtcctcaac
    19441 tattacctgc aatggcagtc ccgccgcgaa taggatcatc catgaaacgc ttttccttga
    19501 aatacacttt actcacctat cgtcttgacc aactttggtt cccgctggca ttcctggcat
    19561 tcttcgtcat cctgactttg cttcttcgca acgaagtgca aaactatgac attgctcgcg
    19621 cctatgtcgg cttcgctgtc ccgctggttg gcggcttcat ggcggcatac gccatcctcg
    19681 atgaccctgc gctggaattg cgctttgcta cacccatccg tgccgcgcag atcttgttcg
    19741 aacgcaccac gctgatcttt ctcattcagg cagtctgcgc caacgccttt ctgcttttct
    19801 gcctcattat cggcgtggac ttttctccgc ttggcaattg ggtgaatgtc caactgtgct
    19861 ggctgctacc caccatcacc ttgatcgcgc tcggaacctt cggcgcattg atcggtgctc
    19921 agaccatggt cggttcattc ctcgtcggtc tcgtctggtt cgtggagttg atcatccgcg
    19981 acgggatggc tgtcaataac tggaaatata cctacatctt catgggcatt ctcgctcccg
    20041 aacatcctga ccttgtatca aatcaaattg cgctcttcgc tctcaccatt gctttcctct
    20101 ttgttgcatg ggcattgctg cgaaaacagg aaaggtatat ttaaacggta ccgcgacatg
    20161 cttcgcaatg tcgcgcttga aaaatccgat gactaaccac ggagtcacgg agacactgag
    20221 aaaatcataa aaactccgtg acttcgtg
//
LOCUS       DAOQJC010000067        20122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1223_length_20122_cov_9.180296, whole genome shotgun sequence.
ACCESSION   DAOQJC010000067 DAOQJC010000000
VERSION     DAOQJC010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 20122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20122
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1223_length_20122_cov_9.180296"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLE39_11335"
     CDS             <1..269
                     /locus_tag="PLE39_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68968.1"
                     /translation="SDTFQCCQAVTGGTYPGCSPGSTFNRDLGACSPGEIRLAGPGCV
                     TLDVTTLKCSQPVDVCSRIKSETTCIRNSYACKWDDEINVCTLR"
     gene            605..1099
                     /locus_tag="PLE39_11340"
     CDS             605..1099
                     /locus_tag="PLE39_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68969.1"
                     /translation="MERVEYVLLLMGAIFCAYRAIVSKRILSSTIYLACISALISVVL
                     FMLGATQVAVMELSVGAGLVTVLLVYALSVVGEDALDPSSVIPKPFAIFVVSAAALFL
                     GWMVYPAVQFDSQMASIDLASVLWQQRVLDVWIQIVLIFSGVMGVLGLLSERTPTGIT
                     GGRA"
     gene            1096..1407
                     /locus_tag="PLE39_11345"
     CDS             1096..1407
                     /locus_tag="PLE39_11345"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HNE68970.1"
                     /translation="MNLTVLNIILLGIIGLLGVGVYGLLITRNLIKVVMVLQILVKAV
                     ILAFVFSGKVSGNLGLGQSIAATVIVADTIVAVVGLALAVQVRRRVGTLDAPQVSTLR
                     G"
     gene            1409..3163
                     /locus_tag="PLE39_11350"
     CDS             1409..3163
                     /locus_tag="PLE39_11350"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNE68971.1"
                     /translation="MAGILILLIIGFPWLGAFVVWAAGDSRPKAQHALAVTFSLFSGI
                     SSLILLAFVNSEPVIDLALGTSFGNLTFSPDGLAVSLTVIATVVGSLAVLFSVDYMHG
                     EAQLGRYYFLVLFFIGAMAGLVLSGNLLFTFFFWEITALCSYGLISFYNDDPKAVAGG
                     IKALIITQVGGVGLLAGALMAYANLGSFQISALLANAESIPTAALSFIAFSFLIAAAA
                     KSAQFPFHTWLPDAMEAPTPISALIHAATMVNAGIYLLARFYPAFESVPAWKITVMLV
                     GVTSALITAFMALTATDLKRTLAYSTVSQLGYMVYAIGAGGILASQFHLFSHAVFKAL
                     LFLTAGSVIHAVGTRDMRRMGGLGAKMPFARNVFIIGALALAGIPIFNGFWSKELILE
                     IGLEHSPIWAYALMLLGAGLTAFYTFRMTWMVFFGTPREALHVHSVGAAMKISLTVLS
                     IGTLTTWLLFGGLNGILSTSLPFHEIETESTLEMIKAISFAPATWFALLVVTLGLGLF
                     FIFGRKFALSESPAWFGMLTESSFGFEPVNRFVVSFVSGGAERLRATQTGQLNWNILA
                     IVGALLVVLAVLVWGGAL"
     gene            3160..4680
                     /locus_tag="PLE39_11355"
     CDS             3160..4680
                     /locus_tag="PLE39_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNE68972.1"
                     /translation="MNILNFVSMITIPLIATPMVYLSGRLGTRETVLHSRSYLVRGLA
                     LLAILLAWIPFVVSARAFLAGGVLEFSIESIWLRMDGISLLVAAMALTLGTLVVLFSG
                     PYMAGEVGEEKYYAMLLAMIGLMIGLACADDLFNLWVWFEGMAVSSYLLVAFYREQSA
                     SLEAGIKYLVQSAVGSVLALLGISLVLANAGTLNMPEVREAVASLSVNRFALLGAGAL
                     FVIGFGVKVAVVPLHTWLPDAHSQAPSGISAMLSGVVIEAGLIAMLRALSVLTGFSNS
                     WGLLFMIFGALNILYGNLLALRQTTIKRLLAYSSLSHIGYILLGLGVAFYSGAALGAQ
                     GAFFHFFTHMLMKGLAFLSVGALMYGMFLQNDSHAALKVTDLAGAAQKYPLVALAFSI
                     AVLALGGLPPLAGFMSKWQIFVAGFQVQNNWVTALMIFMALNSVLSLAYYAPLVNLMY
                     RKSPSEQVMAGKPLPLAINLPLLLMMLAVIVLGFVPQLMDWVTVPAAQVFIAMFGG"
     gene            4684..4998
                     /locus_tag="PLE39_11360"
     CDS             4684..4998
                     /locus_tag="PLE39_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68973.1"
                     /translation="MNNFILTPVFAFLAYALAVSAVSGLARMFSAKGQKSQFKSEPYA
                     SGEDYDPVPAAPGYRQFFVIALFFAVLHLGIIMVGSSDLSSVTIVYLLGLILALVALI
                     LG"
     gene            5016..5525
                     /locus_tag="PLE39_11365"
     CDS             5016..5525
                     /locus_tag="PLE39_11365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNE68974.1"
                     /translation="MNKNNEQFWSQIIDKVRTWARLNSIWAVHFNSGSCNGCDIEILA
                     TLTPRYDVERFGIKLEGSPRHADVLIVTGPVTRQARERLIRTYEQMPEPKFVISVGSC
                     SISGGVFEGCYNVGGSVDEVIPVDVYIPGCPPRPEAIIDGIVQLLASLKGGEPRKMAR
                     PANVEEMPA"
     gene            5522..7195
                     /locus_tag="PLE39_11370"
     CDS             5522..7195
                     /locus_tag="PLE39_11370"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNE68975.1"
                     /translation="MNIEQTLKDAEQFLAPWAKELLHPESNRLDVAIQVDDLRAATSA
                     LLAAHWGYLSAITGTDLGVEVGQLEALYHFCNGPAITSLRIHLPREIDACLPSIADLI
                     PSASFFERELHEMMGFAFHGLDVSSRLFLPDDWPENVYPHRADFSPDRAAPVAGRRAD
                     ELPEQGVVGGNTFVIPIGPQHPALKEPGHFEFTVDGEIVTAAKMRLGYAHRGIEKAAE
                     SRNWIQNLYLFERICGICSHTHSMAYSLGVERLAQVEAPPRAQAIRELVAGLERVHSH
                     LLWLGVAAHEAGFDTLFMYSWRDRETVMNLLEQITGNRVNYSANVLGGVKCDIGAEQA
                     KAVKEGLTYLETRIRHYLEVVTTDAMFLNRTRGIGTISREEAEKFGLLGPTARASGLS
                     RDVRLEAPYGAYHHFPVSLVMETNGDLEARCVVRLKELLVSCFTMRTIVDNLPEGPLT
                     TRMPRKIPAGETVSRIEAPRGELFYYIKSKGGENPARVHIRTPSLCNWVSVLEKAVGH
                     QLADVPMLIAGMDPCFSCNDRVVTVNRPSTNETWSWKQLREHGIEYYKR"
     gene            7192..8115
                     /locus_tag="PLE39_11375"
     CDS             7192..8115
                     /locus_tag="PLE39_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNE68976.1"
                     /translation="MNTFSSFVTLLFFPAGLSLIVVGMFYEWVDRKLVARFQNRIGPR
                     WFQPFADMVKLFAKEQIKPAFVNPYLFFGLPVVALAGALTSALYVPLMGLTPASSFPG
                     DLIVTIYLLSLPTMCVGLAGWNNSGRFSLIGATRALTQLFAYEAPFLLALLGPALVAG
                     SWQINTINRFTQSHWLILTQPVGFIVALIGLMGKLELPPFDAPEAETEIVAGALTEYS
                     GRGLAMFKIGKSVELIVGLCLVASFYLGGLNGVPSFLFKTLILLLSMTMLQSLLTRLR
                     IDQTVGMWWRFGAILVLAQLLVIILVKGFLP"
     gene            8112..8543
                     /locus_tag="PLE39_11380"
     CDS             8112..8543
                     /locus_tag="PLE39_11380"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNE68977.1"
                     /translation="MKIGTMLKDIVASFFKKSSTQLYPFEKTRAPERYRGNLFYDPSA
                     CTGCCLCVKDCPSKAIELVTLDRAAKRFVLKYHMDQCIYCGQCAVNCKFKCMGMTSQD
                     WEHAALDKKEFTVYYGKDEDIKQLLENVSKPAVEPTDNQTG"
     gene            8497..9012
                     /locus_tag="PLE39_11385"
     CDS             8497..9012
                     /locus_tag="PLE39_11385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation endopeptidase HyCI"
                     /protein_id="HNE68978.1"
                     /translation="MSLNQQLNQLTTKLDELPRVAVVGIGNTLRNDDGAGILVARELT
                     RREHLAGMHHVLVIQAGHAPENVTGELREFDPDLILLVDAAEFRDVPGAICWVPIQAI
                     EGMSASTHSLPLSMLARYLTLELHCDVALIGIQPKSNDVGEVISVEVQAAVNEIVNEM
                     CDACLIIKPAY"
     gene            complement(9142..9477)
                     /locus_tag="PLE39_11390"
     CDS             complement(9142..9477)
                     /locus_tag="PLE39_11390"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNE68979.1"
                     /translation="MKTQSGLEYIEVEAGTGAQAVAGKTVSVHYTGKFQDGRVFDSSV
                     SRGEPITFPLGRGNVIKGWDEGIALMKVGGKAQLIIPPHLGYGERGAGGVIPPNATLV
                     FDVELVSVQ"
     gene            9578..10015
                     /locus_tag="PLE39_11395"
     CDS             9578..10015
                     /locus_tag="PLE39_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE68980.1"
                     /translation="MSTVRDMIRKKGSEVFSILPEASVYDALEIMAKHNTGALMVVSG
                     GRVEGILSERDCIRKLDLEGRTAKNTKVSEIMTSKVVYVEAAQPLEECMALMIDKNIR
                     HLPVFDGKDLLGLISVRDVLKEVVDVQKFLISQLEHYITGGGR"
     gene            10017..11516
                     /locus_tag="PLE39_11400"
     CDS             10017..11516
                     /locus_tag="PLE39_11400"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF005300.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNE68981.1"
                     /translation="MQEQTISEFQKKMISGELTSRQLTELFLERINSVDHNGPKLNSV
                     IETNPDALTIASNLDEERRAGKVRSQLHGIPILIKDNIDTHDRMQTTAGSLALEGHIA
                     PRDAFIVKQLRKAGAVILGKTNLSEWANFRGRRSISGWSSRGGLTHNPYALDRSACGS
                     SSGSGAATAANLCAAAVGTETDGSVICPAQTNGIVGIKPTLGLLSRTGIIPIAHSQDT
                     AGPMARTVADAAILLGAMTGTDEKDPSTKRGSKHIAKDYSKFLDPNGLKGARIGVARN
                     MCGTDARIIKIFEASIAVMKQQGAVIVDPANLPNFNKFGATELEVLHYEFKADLNKYL
                     AGSNSKLKTMAEIIKFNEEHKSRVLKYFGQEHMLAAQEKGSLRDKKYREALAKNLLLT
                     RENGIDATLKKYKLDAIVVPSGGPAWMIDLANGDAINWDMESTSPAAVAGYPHITVPA
                     GYVFGLPVGISFFSTAWQEPTLIKLAYAFEQSTQVRKPPQFPATVFGNL"
     assembly_gap    11522..11621
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11622..13607
                     /locus_tag="PLE39_11405"
     CDS             <11622..13607
                     /locus_tag="PLE39_11405"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNE68982.1"
                     /translation="PLPMHLFELFEKPVGFFLVVMAIILITPLLSEKLKLPGIIGIII
                     GGMLIGPHGFGLLEDNDRIQFLSTIGLVYLMFSAGLEVDINQFMKVRTRALVFGVITF
                     AFPQLMGMGLGYILGLDWLGMILLGSAFASHTLIAFPILTKLGVARNEAVAVTTGATV
                     LTDIGAFIVLAVVLGAKSGGGITFGYFAQLLVLLTIFTAAIIFGLPRLGKLIFQKLTG
                     RAVEFQFVIVALFVAAFTAELIGVHEVVGAFLAGLAINSMLPRHSPVAGHVLFVGESF
                     FIPIFLLYSGLITDPLTFLKSPQTIVVAIGVTIVAYVSKLVAAWITARIYKYTKSEFW
                     TVYGLSHAQAAVTIPTLVIGLETGLFDSTLFNAAILMILLTSITSPLIVQRFASDLQT
                     ASADDEQTPLFGRILVPVSDVKQSEGLVSLASLLARTSKGKVLAVSVSNDKGLSNGTT
                     SFGIHKEMLGRVTETLNDPETEIELIPRMASTHAQGILHTSHEENASLIVMGWRGRRT
                     LRENVLGSVLDEVIWGSDTPVMVGKLPLPLNSMTRVIFVVPAASVPPIALRRMLQATV
                     VFAKSLNVPLLVRADSSYLQNLEALLVVTREDHPVTLEVLKDQLKPEKLAHEAVSDFI
                     ILPGFGSRKRVADTLGNIPEQIAKSFEGNLAILHFDK"
     gene            complement(13650..14576)
                     /locus_tag="PLE39_11410"
     CDS             complement(13650..14576)
                     /locus_tag="PLE39_11410"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HNE68983.1"
                     /translation="MLLLTRKEIEHLLDPDELLTVLEEGFKALSAGQLNVPPRNQAIA
                     PKGLLVGMPAYMPNQFMSVKLVTIFHENIPAHQATITLFDSNTGTPIAFMDGEHITAM
                     RTAAGAILSIKHLARKDSKSIAIVGAGVLGQAHLQMIGTLSGIEKIWIASKDISKAQA
                     LAARASNALAVDSVREAVSNADIVCLCTSSLEPVIQAEWLKKGVHVSSVGYRPPGGEL
                     PRDLVEASHIFVESKVAFQAPPVGSAELQGLNAEFGTEMGEYLLGQKPGRRNETETTI
                     YKSMGHAMEDLVTANLVYQKALAQGIGMTFDL"
     gene            complement(14603..15634)
                     /locus_tag="PLE39_11415"
     CDS             complement(14603..15634)
                     /locus_tag="PLE39_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE68984.1"
                     /translation="MTTPLPRAIVVEDDQAWQQIMSEILSDCGLDVDVAGSLEEAIPL
                     LKAHPHRLAVADLSLSANHNNTDGLRVLENIKHLDPECKSILLTGFATVEVAVAALTR
                     HHAHTFLQKESFNRSQFKEIIHNILTSAPKPEEEKAAPAPAQPTLQNPPAMPKALIVD
                     DDAGWRSILEDLLSDLGFQPVLCASFGEAVGALKKNQLTLAIIDLSLNEAVSQNGYPL
                     LGLAQERKIPTVVISGLSEPEEIQRIYSRYSIVAFIEKHAFDRATFKRIITEAKDSPV
                     PDDKLSTLTEREREVLDLLAKGMTNKEIADQLVITTNTVKRHLKAVFEKLEVHTRSAA
                     TAKVTRGSL"
     gene            complement(15631..17796)
                     /locus_tag="PLE39_11420"
     CDS             complement(15631..17796)
                     /locus_tag="PLE39_11420"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE68985.1"
                     /translation="MANSPIPPSEELNPRTLEALASLNQISTAINQITSSEDQSSSLQ
                     LIVDSAIRVVPGFSASIYIYNPNTDRLEIESRVVSYAKGYEEYRHINPNDDVPRSNGF
                     GMRAIQRKRRTLSYEEANLQIHPYQEKLGVKVVACFPLLASKEVLGALYIYLYEERQL
                     SQFEELMLHNFVNQAAMAIYHARHVAGIKHNLAQKENELNRLRHAGMLISSRMRLEET
                     LESILELALELTNAQYGIFRLVDKSGEDLVTRAVSSVHLEKPLVEKLPIKGQSVMAYV
                     ARTRQSLLIRDLREKPWNSVYYPLDSKLEMRSELCIPLINASGRLEGVLNLESPRIGG
                     FSEDDSHMLQSLATYAVTAIQEVRLLDALQEAAQLLISQSSQEVLNTLCGMANDLLNT
                     SSSAVWLANGQGEMELAASNGADFPAQDPQGMDLPNALLMPLLSGENDSAAGIFGVFQ
                     AAPGGVRSDTTEWDKKVLACLANYAVLAIQNESRQQALRSSQEQHLIAETFAAVGDIS
                     ANLLHNMNNKVGTIPVRVQAIQDKYDTLLESDAYLNKSLIEIERSAMAAMQIVRENLS
                     HLRPIRIEQIQVAPRVQDAIHTIQVQPGTRIIISGLDALPTINANAQSLTFVFRNLIE
                     NAMDAMKGNGTISVSGMVIEKNVEVRVADTGPGIKPELQEQIFELEYSSRSHPGKLGF
                     GLWWVRTLMTRLGGSVQVESDGEHGTTFVLKLPIAEVRA"
     gene            complement(17865..18494)
                     /locus_tag="PLE39_11425"
     CDS             complement(17865..18494)
                     /locus_tag="PLE39_11425"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNE68986.1"
                     /translation="MHISLFISSFILAISFCAPPGVVTAETFRRGSARGFFPALYVQF
                     GSLIGDTTWAVIALTGLAFVIQNNIARTILSFIGIALMLKLAWDALKDARHGNGLNIS
                     EPASSRGDFASGAFLSLGNPMNIVFWTGLGTTVFASIAGGPEPIHFVTFFAGFLSGAV
                     LWCFILAGLIAWGRQWMTVGFFRFLNATCGLVLFYFAFQLGIQTMQNFI"
     gene            complement(18589..19995)
                     /gene="glnA"
                     /locus_tag="PLE39_11430"
     CDS             complement(18589..19995)
                     /gene="glnA"
                     /locus_tag="PLE39_11430"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNE68987.1"
                     /translation="MAKSIKEVLEMAKSAQVVDLRFTDLPGTWQHFTMPVHRLTEDFF
                     KEGIPFDGSSIRGFQEIHESDMLLKADPDTAFLDPTAETPTLVLSCNVFDPITEEAYS
                     RDPRYVAQKAEAYAKSTGLADTVYFAPEAEFFVFDGIRYVSTPHEASFSIDSEEGWWS
                     SNREGNKGGQIQPKRGYFPVSPTDTLQELRNKFMLAMQAAGIQMDLHHHEVATAGQSE
                     MSMTFTTLTRMADDLLLYKYIIKNVARKYGKTVTFMPKPIFGDNGSGMHVHSSMWKDG
                     KNVFFDEKGYAGLSQTAKYYIGGLLKHAPALLALTSPTTNSYRRLVPGYEAPVNIAYS
                     QRNRSAICRIPATKSWKAKRVEFRAPDATANPYLAFSAVLMAGIDGILNKIDPGEPKD
                     VDLYELPAAEKALIKQVPSSLDAVLHALEADHEFLLKGGVFTPDLLEEYIKYKRTHEV
                     DAIRLRPTPHEYVLYFDA"
BASE COUNT         4164 a         5079 c         5582 g         5197 t
ORIGIN      
        1 acagtgacac gttccagtgc tgtcaggctg tgaccggtgg aacctacccg ggctgctctc
       61 ctggctccac gttcaatcgg gacctcggag cctgttcgcc tggcgagatc aggcttgccg
      121 gcccgggctg tgtcacactg gatgtgacca cgctcaaatg cagccaaccc gtggacgtat
      181 gttcccgcat caaatctgag accacctgta ttcgtaattc ctatgcctgt aaatgggatg
      241 acgagatcaa cgtctgtacc ttgcggtaaa tgaagtttac ccaacacccc gtggaaaaaa
      301 ttccacgggg tgtttttttt tattgcggaa gtttagtaga ttttctctgc gctctccgcg
      361 cctctgctgt aaaacgggtt tgatcgaaaa catgcatccg taaccctccc acaaaaaata
      421 cccaaaaaca taaaaagcag acgatttagc cgtcatttcc gaatcctaca agcggtgact
      481 aacggtatgg tgggcgggtg attaacggta ctgatgccct tttttgtttg tggtatattt
      541 cacaattgag gatagtatgc ggaggtagag tatttgcagg ctaggttgga ggccgtagta
      601 aaaaatggaa agagtagagt atgtgttgct tttaatgggg gccatctttt gcgcgtatcg
      661 cgccatcgta tcaaaacgga ttctttcgtc aacgatatat ctggcgtgca ttagtgcgtt
      721 gatctcggtt gtgttgttca tgttgggcgc aacccaggtg gctgtgatgg aactttctgt
      781 gggggcgggg ttggtcacgg tgctgttggt gtacgcattg agcgtggtgg gtgaagatgc
      841 cctcgatccc agttcggtca tcccaaaacc gttcgcaatt tttgtggtct cggcagcagc
      901 cctgttcctt ggctggatgg tgtaccccgc tgttcaattc gatagtcaga tggcttcgat
      961 cgaccttgcc agtgtactct ggcagcagcg cgtattggat gtgtggattc agatcgtgct
     1021 gatcttttct ggtgtgatgg gggtgctcgg tctgctttct gagcgaacac caactggaat
     1081 tacaggaggc cgcgcatgaa tctgaccgta ctcaacatca tcctgcttgg catcattggt
     1141 ctgcttggcg tcggggtgta tggacttttg atcacccgca atctcatcaa agtggtgatg
     1201 gtcttgcaga tcctggtcaa ggccgtcatt ctggcctttg ttttttctgg caaagtcagc
     1261 ggcaacctcg ggctgggaca atccatcgcg gcaacggtca tcgttgccga taccattgtg
     1321 gcagtcgttg ggctggcatt ggcggttcaa gtccgtcgtc gagttggcac ccttgatgcc
     1381 cctcaggttt ccacattgag aggatgaaat ggctggcatc ttaattctcc tcattattgg
     1441 ctttccgtgg ctgggtgctt tcgttgtgtg ggccgcaggt gactcacgcc ccaaagccca
     1501 gcatgcactg gctgtgacct tcagcctgtt ttcgggcatt tcctccctga tccttttggc
     1561 atttgtcaat tcagaacccg ttattgacct tgcacttgga acctcattcg gcaacctcac
     1621 cttctccccc gatggtctcg ctgtttcatt gaccgtcatc gccactgttg taggttcgct
     1681 ggcagttcta ttctcggtgg attacatgca cggtgaagcc caacttggac gatattattt
     1741 ccttgtgctg ttcttcatcg gtgccatggc tggcctggtc ttgagcggaa atttgctctt
     1801 caccttcttt ttctgggaga tcacggcact gtgttcgtat gggttgatct ctttctacaa
     1861 cgacgatccc aaagctgtgg cgggcggcat caaagccttg atcatcaccc aggtgggtgg
     1921 tgtggggttg ctcgcgggtg cattgatggc gtatgccaat ctcggcagtt ttcaaatttc
     1981 agccctgctt gcaaacgcag agtcgattcc cactgcggcg ctctctttca tcgcgtttag
     2041 ctttttgatc gccgccgctg caaaatcggc gcaattcccg ttccacacct ggctgcctga
     2101 tgccatggaa gcgccgaccc cgatcagcgc attgatccat gcggccacca tggtgaatgc
     2161 gggtatttat ttgctggctc gtttttaccc cgcttttgaa tccgttcccg catggaagat
     2221 caccgtcatg ctggttggtg tcacttcggc gcttatcaca gccttcatgg cgctgaccgc
     2281 caccgacctc aaacgcacac tggcttattc cacggtcagc cagcttgggt atatggtcta
     2341 tgccatcggc gcaggcggta tattggcaag tcagtttcat ctatttagcc atgcggtttt
     2401 caaggccttg ctcttcctca ccgcaggctc ggtcattcac gctgtaggca cccgtgacat
     2461 gcgccgtatg ggcggccttg gcgcaaagat gccattcgcg cgcaatgtct ttatcatcgg
     2521 tgcgttggca ttggcaggca tccccatctt taacggtttt tggagcaagg aactcatcct
     2581 tgagatcggc cttgaacatt ctccgatctg ggcttatgcc ctcatgctgc ttggcgcagg
     2641 cctcaccgct ttctatacct tccgcatgac ctggatggtc ttctttggca cgccgcgcga
     2701 agcattgcat gttcattccg ttggtgccgc catgaagatc tcattaaccg tgttatccat
     2761 cggcacactc accacctggc ttctctttgg cggattgaat ggaatcttat ccacgtccct
     2821 gccttttcat gagatcgaaa cagaatccac cctggagatg atcaaagcga tcagcttcgc
     2881 acccgcaacc tggttcgctc ttctggtagt gactctcggc ctgggcctct ttttcatctt
     2941 cgggcgaaag tttgcacttt ccgaaagccc tgcctggttc ggtatgttga ccgaatcttc
     3001 gttcggattt gagcccgtca atcgttttgt cgtcagcttt gtttcaggcg gcgctgagag
     3061 attgcgcgcc acgcaaacag gccagttaaa ttggaacatc ctcgccattg ttggagcgtt
     3121 gctggttgtg ctggcggttt tggtatgggg gggagcctta tgaacatttt gaattttgtc
     3181 tcgatgatca cgatcccctt gatcgccacg ccgatggtgt acttgtcggg gaggctggga
     3241 acacgcgaaa ccgttcttca cagccgctcc tatcttgtgc gcggactggc cctgctggca
     3301 attttgcttg cgtggattcc gtttgtggtt tcggcccgtg cctttcttgc tggcggtgtt
     3361 cttgaattta gcattgaatc catctggctg cgtatggatg ggatcagcct gttggtggcg
     3421 gccatggcac tgaccctggg cacgctggtg gttttgttct caggtcccta catggctggg
     3481 gaagtgggcg aagagaaata ttacgccatg cttctggcga tgatcggttt gatgatcggt
     3541 ctggcctgcg ccgatgacct gttcaacctg tgggtgtggt tcgaaggaat ggcggtttcg
     3601 tcctatttgt tggtggcttt ttatcgtgaa caatccgcct ctcttgaggc gggtatcaag
     3661 taccttgtgc agagtgcggt ggggtcggtg ctggcattat tgggtatttc actggttttg
     3721 gccaatgccg ggacgttgaa tatgcctgag gttcgagagg cagttgccag cttaagtgtg
     3781 aatcgttttg ctttgcttgg tgcgggcgca ttgtttgtca tcggcttcgg ggtgaaggtg
     3841 gcagtcgttc cgcttcatac ctggctgccc gatgcgcatt cgcaggcgcc gagtggaatc
     3901 agtgcgatgc tttcgggtgt ggtcatcgaa gctgggttga tcgcgatgtt gcgcgcactt
     3961 tccgtgctga cgggattttc gaactcctgg ggccttttgt tcatgatctt tggtgctttg
     4021 aacattttgt acggcaacct gctggcattg cggcagacaa cgataaaacg cctgctggcc
     4081 tattcgagcc tgagtcacat tggttacatt ttgttggggc tgggtgtggc tttttattcg
     4141 ggcgcagcac ttggggcgca gggtgcgttc tttcattttt tcacgcatat gttgatgaag
     4201 ggtctggcct ttctttctgt gggcgcgctg atgtatggca tgttcctgca aaatgactca
     4261 cacgccgctc tgaaagtgac cgaccttgcc ggggccgcac aaaaatatcc gctggttgcg
     4321 ctggcgttca gcattgccgt gctggccttg ggcggcttgc cgccgttggc gggtttcatg
     4381 tcgaaatggc agatttttgt ggcggggttt caggttcaaa ataattgggt cactgcgttg
     4441 atgattttta tggcgctcaa cagtgtgctt tcactggcat attacgcgcc gttggtgaat
     4501 ttgatgtatc gcaagtcgcc ttccgaacag gtgatggcgg gcaagccttt gccgctggcg
     4561 atcaacctgc cgttgttgct catgatgctt gcagtgattg tgcttggctt cgttccgcag
     4621 ttgatggatt gggtcactgt ccctgcggcg caggtattta ttgcgatgtt tggaggatag
     4681 gacgtgaata attttattct cacacccgtg tttgccttcc tcgcctacgc tttggcggtt
     4741 tctgcagttt caggcctggc gcgcatgttt tcggcaaaag ggcagaaatc acaattcaag
     4801 tcggagcctt atgccagtgg tgaagattat gaccctgtgc ctgccgcccc gggctatcgt
     4861 cagttttttg tgatcgcctt gttctttgcg gttttgcatt tgggaatcat catggtcggc
     4921 agcagtgacc tttcttctgt caccatcgtt tatttgctgg ggttgatcct ggcgctggtg
     4981 gccttgattc tcgggtaaag gtttgaaggg aaaccatgaa caaaaacaac gaacaattct
     5041 ggagtcagat cattgataaa gtgcgcacct gggcgcggtt gaattcgatt tgggctgtgc
     5101 atttcaacag cggatcgtgc aacggctgcg atatcgagat ccttgccacg ctgaccccgc
     5161 gctacgatgt ggaacgcttc ggcatcaaac tggagggcag tcctcgtcat gctgacgtgc
     5221 ttatcgtgac tggaccagtt acacgtcagg caagggagcg tttgatccgc acctatgagc
     5281 agatgcccga gccgaagttc gtcatttcgg ttgggtcgtg cagcatttca ggcggcgtct
     5341 tcgaaggttg ttacaacgtg ggcggttctg tggatgaggt cattccagtg gatgtgtaca
     5401 tccccggttg cccgccgcgc cctgaggcga tcattgatgg cattgtgcag ttgcttgcca
     5461 gtttgaaggg cggtgaaccg cgcaagatgg cgaggcctgc caacgtagag gagatgcctg
     5521 catgaatatc gaacaaactt tgaaggatgc cgaacaattt ctcgctccat gggcaaagga
     5581 attgcttcac ccggagtcaa accgtttgga tgtggcgatt caagtggatg acctgcgtgc
     5641 cgccacgagc gctttgctgg ccgctcattg gggctattta tcggcgatca ctgggacgga
     5701 tcttggcgtt gaggttggtc agttggaggc gctttaccat ttttgcaacg gccctgcgat
     5761 cacatcgctg cgaatccatt tgcctcgtga gatcgatgcc tgcctgccaa gcatcgcgga
     5821 cctcatccca tcggcttcat tttttgaacg tgaattgcac gagatgatgg ggttcgcatt
     5881 ccacggattg gatgtgagca gccgcctgtt tttgccagat gactggcctg aaaatgtgta
     5941 cccgcatcgg gcggattttt cacccgatcg tgccgcgccg gtggctggtc gccgcgccga
     6001 cgaattgccc gagcagggag tggtcggcgg gaataccttc gtcattccca ttggcccgca
     6061 gcatccagcg cttaaggaac ctggtcactt tgaatttacg gtggatggag aaattgtcac
     6121 cgccgccaaa atgcggcttg gatatgccca ccgtggaatt gaaaaagccg ccgaaagccg
     6181 taactggatt cagaacctgt atctctttga aagaatttgc ggaatctgtt cacacaccca
     6241 ttccatggct tacagcctcg gcgtggaaag gctggcgcag gtggaagctc cgccgcgcgc
     6301 gcaagccatc cgagagttgg tggcaggctt ggagcgcgtt cacagccatt tgttgtggct
     6361 gggcgtggcc gcacacgaag caggttttga tacgttgttc atgtattcgt ggcgtgaccg
     6421 cgagacggtg atgaatctgc ttgagcagat cactggcaac cgcgtcaatt attccgcgaa
     6481 tgtgttgggc ggcgtaaagt gtgacattgg tgcagagcag gccaaagccg tcaaagaagg
     6541 gctgacctat cttgaaaccc gcatccgcca ttatctggaa gtggtcacca ccgatgcaat
     6601 gttcctgaat cgaacacgcg gcattggcac catctctcgg gaagaagcgg aaaaattcgg
     6661 cctgcttggc ccgactgcac gcgcttccgg tctgtcgcgc gatgtacgcc ttgaagcgcc
     6721 ttacggtgcg tatcatcatt tcccagtcag tcttgtgatg gaaaccaatg gcgatctgga
     6781 agcccgctgt gttgtccgtt tgaaggaatt gctggtctca tgctttacca tgcggacgat
     6841 cgtggataac ctgccagaag gtccgctgac aactcgtatg ccgcgcaaga ttcccgccgg
     6901 agaaacggtt tcccgcatcg aagcgccgcg tggagaattg ttctactaca tcaagagcaa
     6961 aggcggcgaa aaccccgcac gggttcacat ccgcactccc agcttgtgta attgggtttc
     7021 ggtgctcgaa aaagctgtgg ggcatcaact ggccgatgtg ccgatgttga tcgctggcat
     7081 ggacccatgt ttttcgtgca atgaccgtgt ggtcacagtc aatcgtccca gcaccaatga
     7141 aacctggtcg tggaaacaat tgcgcgaaca tggaatcgaa tattacaaac gatgaacaca
     7201 ttttcctcct tcgttaccct tctgtttttc cccgccgggc tgtcgctgat cgtagtcggc
     7261 atgttctatg aatgggttga ccgcaaactg gtggcccgtt ttcaaaaccg cattggtccg
     7321 cgctggtttc agccttttgc cgacatggtc aaattgtttg cgaaggaaca gatcaaacct
     7381 gcttttgtga acccgtatct gttctttggc ctgcctgtgg ttgccctggc tggggcgctg
     7441 acttccgcct tgtatgtgcc attaatggga ttaacacccg cttccagttt ccccggtgat
     7501 ctgatcgtga cgatttacct gctcagcctg ccgaccatgt gtgtgggttt ggctggctgg
     7561 aacaacagcg gacgcttctc gttgatcggc gccacacgcg cattgaccca gttgtttgcc
     7621 tacgaagcgc cgtttttact ggccctgctt gggcctgctt tggtggctgg cagttggcag
     7681 atcaacacca tcaaccgctt tacgcaaagc cattggctta tcctcaccca gcctgttggg
     7741 ttcattgtgg cattgatcgg tttgatgggc aaacttgaac tccctccctt tgatgccccc
     7801 gaagcagaga ctgagatcgt cgctggcgcg ctgaccgagt acagcgggcg cggccttgcc
     7861 atgttcaaga tcggtaaatc ggtggagttg atcgttgggt tgtgcctggt ggcttcgttc
     7921 tatctgggcg ggttgaacgg cgtcccgtcc ttcctgttca aaacgctgat cctgcttttg
     7981 agtatgacca tgcttcagtc attgttgacc cgtttgcgca tcgaccagac cgttgggatg
     8041 tggtggcggt ttggggcgat cctggtgttg gcgcagcttc ttgtcatcat tttagtgaag
     8101 ggatttctgc catgaagatc ggaaccatgc tcaaggatat tgttgcatcg tttttcaaaa
     8161 agagcagcac ccagctttat cctttcgaaa agacccgcgc ccctgagcgc taccgcggca
     8221 acctgttcta cgatccatct gcttgcacgg ggtgttgcct gtgtgtgaag gattgtccat
     8281 cgaaagccat tgagctggtc acgcttgacc gtgctgccaa gcgatttgtg ttgaaatatc
     8341 acatggacca gtgcatttac tgcggtcaat gcgctgtgaa ttgcaagttc aaatgcatgg
     8401 ggatgaccag tcaggattgg gaacacgcgg ccctcgataa aaaggagttt acggtttact
     8461 atggaaaaga tgaagatatc aaacagctct tggaaaatgt ctctaaacca gcagttgaac
     8521 caactgacaa ccaaactgga tgaattgccg cgcgtggcgg tggttgggat cggaaatacg
     8581 ctgcgcaatg acgatggcgc tgggattctc gttgcccgag aattaacccg tcgtgagcat
     8641 ttggcgggga tgcatcatgt gcttgtgatt caagcaggcc atgcccccga aaatgtgaca
     8701 ggcgaactgc gcgaatttga tcccgacctg attttgttgg tggatgccgc cgaatttcgg
     8761 gatgtgcctg gcgcgatctg ctgggtgccg attcaagcca tcgaaggaat gagcgcttcc
     8821 acccatagcc tgccgctttc catgctggcc cgctacctga ccctggaact tcattgcgat
     8881 gtggccctga tcggtattca accgaaatcg aacgatgtcg gcgaggtcat aagtgtagag
     8941 gttcaagctg ccgtgaatga gatcgtcaac gaaatgtgtg atgcctgcct tatcataaaa
     9001 ccagcctatt gatcgtaggc caggttttgg tcttgaagaa aagcccgccc tgcaaaaagc
     9061 agccgatgca aattattttg catcggctgt tctcgtttca aattcggttt agaaaattcg
     9121 tgtaattcgc ggcgaatttt tttactgcac cgaaaccaat tccacatcaa agaccagggt
     9181 ggcattgggg ggaatgaccc cgccagcgcc tctttcaccg taccccaggt ggggcggaat
     9241 gatcaactgc gccttgccgc cgaccttcat cagcgcgatg ccttcatccc agcctttgat
     9301 gacgttaccg cgccccagcg gaaatgtgat cggctctccg cgtgaaacgg acgaatcgaa
     9361 cacacgccca tcctgaaact tgcctgtgta atgcacactg acggtttttc ccgccacggc
     9421 ctgtgcgcct gtccctgctt caacttcaat gtattccaat ccgctttgtg ttttcatgat
     9481 tctcctgaag aatgaaattt tttcgcaatt gtacccgttc ttgtcaggtt atggatttca
     9541 gcctatactt tgaaaaaaat cagccgagga gatgtctatg tctacagtgc gcgatatgat
     9601 ccgtaaaaaa gggagcgagg ttttttccat cctccccgaa gcaagcgtgt acgatgcact
     9661 cgaaatcatg gcaaaacata acacaggcgc gttgatggtg gtaagtggcg gcagggtgga
     9721 gggcattctt tctgagcgtg actgcatccg caagctggat ctggaaggca ggaccgccaa
     9781 gaacaccaaa gtcagcgaga tcatgacctc gaaggtggtg tatgtggaag ccgcccagcc
     9841 gctcgaagaa tgcatggcgc tgatgatcga caaaaacatc cgccatctgc ctgtctttga
     9901 tggcaaggat ttgctcggcc tcatctctgt ccgcgacgtt ttgaaagagg tggtggacgt
     9961 gcagaagttc ctcatctccc aactcgaaca ctacatcact ggcggcgggc gctgatatgc
    10021 aagaacagac catttcagaa tttcaaaaga aaatgatctc aggtgagttg acttcgcgcc
    10081 aactcaccga attgtttctg gagcggatca actctgttga ccacaacggc cccaaactga
    10141 attccgtcat tgaaaccaac ccagacgccc tgaccatcgc ttcaaacctt gacgaagagc
    10201 ggcgtgctgg caaagtacgg agtcagctcc acggcattcc catcctgatc aaagacaaca
    10261 tcgacaccca tgaccgaatg caaaccacag caggttcgct tgccttggaa ggtcacatcg
    10321 ccccacgcga tgccttcatt gtcaagcagc ttcgcaaggc tggcgcggtc attctgggta
    10381 aaaccaattt gagcgagtgg gctaattttc gcggcaggcg ttccatcagc ggatggtctt
    10441 ctcgcggcgg cctgacacac aatccctatg cgttggatcg ctccgcttgc gggtcatctt
    10501 ctggttcagg cgcagcgacg gcggcaaacc tttgtgcggc ggcagtcggc accgaaaccg
    10561 atggttcggt catttgccct gctcaaacga atggcattgt gggaatcaaa cccacgctgg
    10621 gcctgttgag ccgcactggg atcattccca tcgcgcacag tcaggatacg gctggcccca
    10681 tggcaaggac tgttgcagat gcggcgattc ttctcggggc catgacaggc acggatgaga
    10741 aagatccgtc cacaaagcgt ggatcaaagc atatcgcgaa ggactattcc aaattcctcg
    10801 atcccaacgg gttgaaaggt gcgcgcatcg gtgtggcgag aaatatgtgt gggaccgatg
    10861 cacgcatcat caaaattttt gaagccagca tcgcggtgat gaaacagcag ggtgcggtga
    10921 ttgttgaccc cgcaaacctg ccgaacttca ataaattcgg tgccaccgaa ttggaagtgc
    10981 tgcattacga attcaaagcc gacctgaaca agtatcttgc tggttcgaat tccaaattga
    11041 agacgatggc cgagatcatc aagttcaatg aagaacataa atcgcgtgtg ctgaaatact
    11101 ttggtcagga acacatgctc gcggcacagg agaagggcag cctgcgcgat aaaaaatatc
    11161 gagaagcgct tgccaaaaac cttttgctta cccgtgaaaa tgggatcgat gccacactga
    11221 aaaaatacaa actcgatgcc atcgtggtcc catcgggcgg ccctgcatgg atgatcgacc
    11281 tcgccaacgg cgacgccatc aactgggaca tggaaagcac atctcctgcg gcggtggcgg
    11341 gatatccgca catcactgtg ccagcgggct atgtctttgg gctgcctgtg ggtatctcat
    11401 ttttctcaac cgcctggcag gaaccgaccc ttattaaact tgcctatgcc ttcgagcaat
    11461 ccacacaggt gcgcaaacct ccgcaattcc ctgccacggt gtttggtaat ttgtaattgg
    11521 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncctctcccc atgcatctct
    11641 tcgaactctt cgaaaaaccc gtcggctttt tcctcgttgt catggcgatc attctgatta
    11701 cgccactgct ttcagaaaaa ttgaaattgc ctggcatcat cggcattatc atcggcggta
    11761 tgctcatcgg tccgcacgga tttgggctgc tggaagataa cgaccgcatc cagtttcttt
    11821 ccaccatcgg tcttgtgtat ctcatgttca gcgcaggcct tgaagtggat atcaaccagt
    11881 tcatgaaagt gcggacgcgc gcccttgtct ttggcgtgat cacgtttgcg ttcccccaac
    11941 ttatgggcat ggggctgggc tacatccttg gccttgactg gcttggcatg atcctgcttg
    12001 gttctgcgtt tgcctcgcac actttgatcg cctttcccat cctcaccaaa cttggtgtgg
    12061 cgcgcaacga agccgtggct gtcaccactg gcgcaaccgt cctgaccgat atcggcgcat
    12121 tcattgtcct tgcagtggtg ctgggagcaa aatcaggcgg cggcattacg tttgggtatt
    12181 ttgcccaact gcttgttttg ctaaccattt tcactgccgc gatcatcttt ggactgccac
    12241 gcctcggtaa actcattttc caaaaactga caggccgcgc tgttgaattt caattcgtca
    12301 tcgtggcgtt atttgtcgcc gcatttaccg ccgaactcat tggcgtgcat gaagtggtcg
    12361 gcgcatttct tgcagggctg gcgatcaact ccatgctgcc gcgtcacagc cctgtggcag
    12421 ggcatgtgct tttcgtcggt gaatcgtttt tcattccgat ttttttgctt tacagcggcc
    12481 tcatcaccga cccgttgacc ttccttaaaa gcccgcagac cattgtcgtt gccatcggcg
    12541 tcaccatcgt tgcgtatgtt tccaaactcg tcgccgcatg gatcaccgcc agaatttaca
    12601 aatacaccaa atctgaattt tggaccgtct acggcctttc gcatgcacag gctgcagtca
    12661 ccatcccgac tctggtcatc ggtttggaaa ctggactttt tgactcaact ctgttcaatg
    12721 ccgccatttt gatgattcta ctcacctcga tcacctcacc cttgatcgtc caacgcttcg
    12781 cttctgacct gcaaactgct tctgccgacg atgagcagac cccgctcttc ggcaggatcc
    12841 tcgtccctgt ttcagatgtg aaacagtcag aagggttggt ttcgctggcc agcctgttgg
    12901 cgcgcacttc caaaggtaaa gtgctggccg tgagcgtttc caatgacaaa ggattgagca
    12961 acggcacgac cagttttggc attcacaaag aaatgcttgg gcgtgtcacc gaaaccttga
    13021 atgaccccga gaccgagatc gaactcattc cgcgtatggc ttcaacccac gcgcagggaa
    13081 ttttgcacac ctcgcatgaa gagaacgctt cgctcatcgt catggggtgg cgcggcaggc
    13141 gcaccctgcg tgaaaatgtg ctcggctctg tgttggatga agtcatctgg ggttcagata
    13201 cacccgtcat ggtcggtaaa ctgcccctgc cgttgaacag catgacgcgc gtcatttttg
    13261 tggtgcctgc ggcttccgtt ccgccgatcg ctttgcgccg catgttgcaa gccacggttg
    13321 tttttgccaa atcattgaac gtccccctgc ttgtccgtgc tgattcaagt tacctgcaaa
    13381 accttgaagc cttgctcgtc gttaccagag aggatcatcc cgttacgctt gaggttctca
    13441 aggatcaact caaacctgaa aaattggcac acgaagcggt cagcgatttt attattctgc
    13501 cagggttcgg ttcacgcaaa cgtgtggccg atactttggg caatatcccc gaacagatcg
    13561 ccaaatcttt tgagggcaat ctggcgattc tgcattttga taaatagaag atggaaaatg
    13621 gaccgtaaac tgtggtttac ggtccatttt cataaatcaa acgtcattcc gattccctgc
    13681 gcaagtgctt tttgatagac caaatttgcg gtgaccagat cttccatggc gtggcccatg
    13741 gatttgtaga tggtcgtttc ggtttcgttc ctgcggccag gtttttgacc gagcaggtat
    13801 tctcccattt ccgtcccgaa ctctgcattc agtccctgca attcggcgct gccaacgggc
    13861 ggcgcttgaa atgcgacttt tgattccaca aagatatggc ttgcttccac gaggtcacgc
    13921 ggtaattctc cgccaggcgg gcggtaaccg accgatgaaa cgtggacgcc ttttttgagc
    13981 cattcagcct ggataactgg ttccagcgaa gaggtacaca aacagacaat gtctgcgttg
    14041 gaaactgctt ctcggacgga atccactgcc agtgcgtttg aagcgcgggc ggccaatgcc
    14101 tgggctttgg aaatgtcctt cgaagcgatc cagatctttt cgatgccgct caaagttccg
    14161 atcatttgta aatgggcctg ccccaacact cccgcgccga caatggcaat ggatttgctg
    14221 tctttgcgcg ccagatgttt gatggagagg attgcgcctg cggcggtgcg catggcggtg
    14281 atatgttcgc catccatgaa ggcgatgggt gtgcctgtgt ttgagtcgaa gagtgtaatg
    14341 gtggcttgat gcgcggggat gttttcgtga aagatggtga cgagcttgac cgacatgaat
    14401 tggttcggca tgtaggcggg catgccaacc agcaggcctt tcggcgcgat ggcctgattg
    14461 cgcggcggca cattgagttg acctgcactg agtgccttga agccttcttc cagcacggtc
    14521 agaagctcgt cggggtcaag caggtgttcg atttctttgc gggtgagaag cagcatggat
    14581 acctcgcgcg atgcagttgt gattacaaac ttccacgggt cactttggca gtggcggcag
    14641 agcgggtgtg gacttccagt ttttcaaaaa cggctttcaa atgccgcttg accgtgttgg
    14701 tggtgatgac aagttgatct gcgatctctt tgtttgtcat tcccttggca agcaggtcaa
    14761 gaacttcgcg ctcgcgttcg gtcagggtgc ttagtttgtc gtctgggacg ggagagtctt
    14821 ttgcttctgt gatgatgcgc ttgaacgtgg cacggtcaaa ggcatgtttt tcgataaatg
    14881 ccacaatgga atatctggaa tagatgcgtt gaatctcttc gggttctgaa agcccgctga
    14941 tgaccactgt ggggattttc ctttcctgtg ccagccccag gagtggataa ccattttgcg
    15001 aaacggcttc attgagcgaa aggtcaatga ttgccagtgt gagctggttt tttttcagtg
    15061 cgcccactgc ttcgccaaaa ctggcgcata aaacaggttg aaagccgagg tcgctcaaca
    15121 gatcttcgag aatactgcgc cagcctgcat catcgtccac aattagggct ttgggcatgg
    15181 cgggcgggtt ttgcagggtt ggttgtgcag gtgcaggcgc agctttttcc tcttcaggtt
    15241 ttggcgcgct ggtcaggatg ttgtgaatga tctccttgaa ctggctgcga ttgaagcttt
    15301 ccttttgcag gaaggtgtgg gcatggtggc gggtcaatgc agcaacggcc acttccacgg
    15361 tggcgaaacc tgtcagcaga attgacttgc actcagggtc aagatgtttg atattttcaa
    15421 ggacgcgcaa tccatccgta ttgttgtgat tggctgaaag tgaaaggtcg gctacggcaa
    15481 ggcggtgcgg atgtgctttg agcagcggta tggcttcttc aaggctgcct gccacatcca
    15541 catccaggcc gcaatcggag agaatctcgc tcatgatctg ctgccaggcc tgatcgtctt
    15601 caaccacaat ggcgcgggga aggggcgttg tcatgcacgc acctccgcga tgggcagttt
    15661 caatacaaag gtggttccat gctcgccatc gctttccact tgcacagacc ctccaagccg
    15721 tgtcatcaat gtgcgcaccc accaaagccc gaatccaagt ttgcctgggt gtgaacggct
    15781 cgaatattcc aattcgaaga tctgctcctg caattctggc ttgatccctg gccccgtatc
    15841 cgcaacgcgg acctcgacat ttttctcgat caccattccg ctgaccgaga tcgtcccatt
    15901 gcctttcatc gcgtccatgg cattctcgat cagattccga aacacgaaag tcaggctttg
    15961 ggcattggca tttatggtgg gcaatgcatc aaggccgctg atgatgatgc gcgtacctgg
    16021 ctgcacttga atggtgtgga tcgcatcctg cacgcgcggc gcaacctgaa tctgttcaat
    16081 gcggatcggc cgcaggtgtg aaaggttttc acgcacgatt tgcatcgccg ccattgcact
    16141 gcgctcgatt tcgatcaaac tcttgttgag gtaggcgtca ctctcaagca gggtgtcgta
    16201 tttatcctga atggcctgca cacgcacagg gatcgtcccc actttgttgt tcatgttgtg
    16261 caaaagattc gcggagatat ctcccaccgc ggcgaatgtt tctgcgatca gatgctgttc
    16321 ctgcgacgat cgcaaagctt gctggcgcga ctcattctgg atggcgagca cggcatagtt
    16381 ggcaagacag gccagcactt tcttatccca ttcagtggta tcgcttcgga ctccgcccgg
    16441 ggctgcttga aacaccccaa atattcccgc cgctgagtca ttttccccgc tcaagagcgg
    16501 catgagcaac gcattgggca aatccattcc ctgtggatct tgcgcgggaa aatccgcacc
    16561 gttggaggcg gctagttcca tttcgccctg gccattggcc agccaaaccg cactcgacga
    16621 ggtgttgagc aggtcatttg ccattccgca aagtgtgttc aacacctcct ggctggattg
    16681 tgaaataagc agttgcgccg cttcctgcaa tgcatcaagc aggcgcacct cttgaattgc
    16741 ggtgacagca taagttgcaa gcgattgcag catgtgactg tcatcttcag aaaatccgcc
    16801 gatgcgcgga ctttcgagat ttagaacccc ttcaaggcgt ccgctggcat tgatgagcgg
    16861 aatgcacaac tcggagcgca tttccagttt tgaatcgagc gggtagtaca cggaattcca
    16921 cggcttttca cgcaaatcgc ggatgagcag ggattgtcgg gtacgggcta cgtacgccat
    16981 cacgctctga cctttgatgg gcagtttttc aaccagcggt ttttccaaat gcaccgaact
    17041 gaccgcacgt gtcacaagat cctcgccgct tttatccacc agacggaaga tgccgtactg
    17101 ggcattggtc aattccagtg caagttccag gatggattcg agagtctcct caagccgcat
    17161 tcgcgaagag atgagcatcc ctgcatggcg cagacggttg agttcatttt ctttttgtgc
    17221 gaggttgtgt ttaattcccg ccacatgcct ggcgtggtaa atcgccatgg ctgcctgatt
    17281 gacgaaatta tgcagcatca attcttcgaa ttgggatagc tggcgttcct cgtacaggta
    17341 aatgtaaagt gcgccaagca cctctttgga agccagcagc ggaaaacaag ccacaacctt
    17401 caccccaagt ttttcctgat aggggtggat ctgcaggtta gcctcttcat aggaaagggt
    17461 gcggcgtttg cgttggatgg cacgcattcc aaagccattt gagcgcggca catcatcgtt
    17521 tgggttgatg tggcggtact cttcgtagcc tttggcatat gaaacgacac gagattcgat
    17581 ctcaagcctg tcggtgtttg gattgtaaat ataaatggag gctgaaaacc caggcaccac
    17641 acgaatggcg ctatcaacga tcaattgcag gctggaagat tggtcttcgc tggaagtgat
    17701 ctgattgatg gccgtgctga tctgattcag actcgccaat gcttcgagag tccgtgggtt
    17761 gagttcttcg gaagggggga tgggtgaatt tgccatggag cgcagatctt tacaggtgtg
    17821 attttacttc atggctgcaa ctcggttgag acctgaatgc atggttagat gaaattttgc
    17881 atggtctgta ttccaagctg gaaggcaaag tagaacaaca caagtccgca ggtggcgttg
    17941 agaaatcgga agaagcctac ggtcatccac tgcctgcccc aggcgatcag cccggcgagg
    18001 atgaagcacc acaaaacggc gccggaaaga aaccctgcaa agaaagtgac aaaatggatt
    18061 ggttctggtc cgcctgcgat ggaggcgaaa acggtggtgc cgaggcctgt ccagaagacg
    18121 atgttcatgg ggttgccaag cgagagaaag gcgccgctgg caaaatctcc gcgtgaagaa
    18181 gcaggttcag agatgtttaa tccatttccg tgacgggcat ctttgagcgc atcccaggcg
    18241 agtttgagca tgagggcaat cccgatgaag ctgaggatcg ttcgcgcaat attattttga
    18301 atgacgaagg ccagcccggt gagtgcaatc accgcccagg tggtatcgcc gatcaaagaa
    18361 ccgaactgca cgtataaagc gggaaagaat ccacgggctg agccgcggcg gaaagtctct
    18421 gcggtgacga caccaggcgg ggcgcagaag gaaatggcga gtatgaaaga agagatgaac
    18481 aatgaaatat gcatggtgtt tctttggtag aaaaatgccg acagtcagat gtgtgtcagg
    18541 ggcacaccca actgtcggcg gggagtgaag cgattttagt cgggctgatt aggcgtcgaa
    18601 gtagaggacg tactcgtgcg gagtcgggcg caggcgaatg gcatccactt cgtgagtgcg
    18661 tttgtacttg atgtactctt cgagcaggtc gggggtgaac acgccgccct tgaggaggaa
    18721 ttcgtgatcg gcttcgagtg cgtggagcac ggcatcgagg ctggaaggca cctgcttgat
    18781 gagcgccttt tctgcggcgg gaagttcgta cagatccaca tccttgggtt cgccagggtc
    18841 gatcttgttc aggataccgt caatacctgc cataagaaca gcggagaaag caaggtaggg
    18901 gttggcggtg gcatcgggtg cgcggaattc aacgcgcttg gccttccaac tcttggtggc
    18961 agggatgcgg cagatggcag agcggttgcg ctgtgagtag gcgatgttta cgggtgcttc
    19021 gtagccgggc acgaggcggc ggtacgagtt ggtggtgggg gatgtcagcg cgaggagggc
    19081 gggagcgtgc ttgagcaagc cgccgatgta gtatttggcg gtctgagaga gacctgcata
    19141 ccccttctca tcgaagaaaa cgttctttcc atccttccac atcgaagagt ggacatgcat
    19201 accggaacca ttgtcgccga agatgggttt gggcatgaag gtcacggtct tgccgtattt
    19261 gcgtgccacg ttcttgatga tgtacttgta aagcaaaaga tcatcagcca tgcgggtgag
    19321 cgtggtaaag gtcatactca tttcgctctg accggcggtg gcaacttcat ggtggtgtag
    19381 gtccatctga atgccagccg cttgcattgc cagcatgaat ttgttgcgca gttcctgcag
    19441 ggtgtcggtt ggtgaaactg ggaagtagcc gcgcttgggc tgaatctggc cgcccttgtt
    19501 gccttcacgg ttgctgctcc accagccttc ttccgagtca atggagaagg aagcttcatg
    19561 cggggtggat acatagcgga ttccgtcgaa gacgaagaat tctgcttcag gcgcgaagta
    19621 gaccgtatcg gcgaggccag tgcttttggc atatgcctca gctttttgcg caacatagcg
    19681 cggatcgcgt gaataggctt cttctgtgat cgggtcaaaa acgttgcagg acagaacgag
    19741 ggtgggtgtt tctgcggtcg ggtctaagaa agcggtatca ggatctgctt tgagaagcat
    19801 gtctgattcg tgaatttcct gaaagccgcg gatggatgag ccgtcgaaag gaatgccttc
    19861 cttgaagaag tcttctgtga ggcggtggac cggcatggtg aaatgctgcc aggttccggg
    19921 taggtcggtg aagcgcaggt caacgacttg cgcgcttttt gccatttcca aaacttcctt
    19981 gattgacttt gccatggttg attttctcct tgcactgatt tattttgact gcgcgattat
    20041 aggggggatg gcacgggtaa gtctattacc caaacgggta cttaatggga aagtggacgg
    20101 ggaagcggcc cgtccacttt tg
//
LOCUS       DAOQJC010000068        20002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1231_length_20002_cov_9.277535, whole genome shotgun sequence.
ACCESSION   DAOQJC010000068 DAOQJC010000000
VERSION     DAOQJC010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 20002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20002
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1231_length_20002_cov_9.277535"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..898
                     /locus_tag="PLE39_11435"
     CDS             89..898
                     /locus_tag="PLE39_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNE68988.1"
                     /translation="MDKTILEIPTDLLEAAKLTADEAKTELAIRLYQLHKLNDEQAGV
                     LAGNPKLIEEMAWNNRSTGRFDMDEFLSWASHDLKTPLNAIIGFTRVVMKGMDGPVND
                     IQTTDLTTVYNGGQRMLALIGNLVDMARLNNSEITLNREPCELRSILVNTADQWQVKN
                     PNKNLTTDIIIHEPVYNIDLARVNQIVTSLLSLAATRVVEGSLSLSARDNDNGAVITV
                     QSTGRKSRDKFEMDSAMLGFISSSLIKLHGGNTESVEETEDGMMIVFHLPR"
     gene            960..2567
                     /locus_tag="PLE39_11440"
     CDS             960..2567
                     /locus_tag="PLE39_11440"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNE68989.1"
                     /translation="MSQIYDYVIIGSGFGGSVSAMRLAEKGYSVLVLEKGKRFNDKDF
                     ATSNWQYWKYVWIPALRAHGILQIAILKGVMVLHGAGVGGGSLGYANVLEVPNEETFA
                     TPAWNQNIKWGDVLRPHYETAKKMLGAARNPKLWKADLLLKDFAEERGMGHTFRATDV
                     GAFFGEAGVTVPDPFFGGEGPNRAGCQHCGGCMVGCRHNAKNTLPKNYLYFAEKKGAQ
                     VKAELEVMDVKPLPTALETGARYEVHYRPSTSLLKSQHIVHARNVIFSAGVMGTMKLL
                     LNLRDVKKSLPKLSARLGTMVRTNSEGLLGSVARRSDINYSEGVSISSIYNHDEITRI
                     EPVRYPDGSSLMRFLAAPLIDNNASIPMRIIKFLGWALTHPIDFAKAAFLPGWAHNVT
                     ILLVMQNADNRMRFRMGRSLFTLFRTGLVADEEPGFKINAQVKGSHEITREFAKRTNG
                     VALGSIGENLLGLPTTAHILGGAPIGKSPEEGVVNENFAVHNYPGLYIIDGSIMPANP
                     GVNPSLTITALAEYVMSKVPPRSATTD"
     gene            2604..3194
                     /locus_tag="PLE39_11445"
     CDS             2604..3194
                     /locus_tag="PLE39_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68990.1"
                     /translation="MPESIMEYWKFDEADLGANRLGQLTEKQKKYLVGEHKSQRSVFL
                     GVGGVVAFLFLCLPALLIGSRVVLPMLLNGDLSDLQDILPLAAMGGFGIVFTGIAVLT
                     VGAVVAIYVMRASKPAEITVTTARGKVEYTWGTKRVSTPGSSVRSYEDVKVLHLNLGD
                     KKFEVKESLQELIKEGEDWSVYYTSYPFKFLSAEKK"
     gene            3201..4472
                     /gene="kynU"
                     /locus_tag="PLE39_11450"
     CDS             3201..4472
                     /gene="kynU"
                     /locus_tag="PLE39_11450"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HNE68991.1"
                     /translation="MSFSTAREFAQQLDTHDRLAAIRNEFVIHDPNLIYLDGNSLGLL
                     PKAAQAKARQVVEEQWGVDLIRRWNKGWWDSPARVGDKIGQLIGAAAGQVLVNDTVSL
                     NLFKLAAAALTLQPSKTRIITDTFNFPSDLYILQGIRQTFGGRHEIISIGASDNDITP
                     DLAALETAIDENTALVTLSHVTFKSGYLYDMQRITELAHKKGALVLWDLSHSVGALPI
                     HLDSCNADLAIGCTYKYLNGGPGAPAFLYVNKSIQEKLSSPIWGWWGQNNPFDFSLNY
                     QPARGAERFLVGTQPILSLLTMEAALEPTLQAGMDSIREKSILMTGYASFLTESWLAP
                     FGFSLGSPPDPAKRGSHISIRHADGYRINRALIEEMNVIPDFREPDNIRLGFAPLYTS
                     FTDVWEGFERIKRVMEEKRHEKYSKQKLTVT"
     gene            4519..5163
                     /locus_tag="PLE39_11455"
     CDS             4519..5163
                     /locus_tag="PLE39_11455"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNE68992.1"
                     /translation="MTTKPDTIKISIMEKEEISATHADLHVTIKGTSLVSGSEAMKKA
                     KEVNQLVEALTSLGLKSEAFFLQGVHLEQAGGALLKSSSAVYRLKIRCEDLEKLSAIL
                     DGISAQKNATLERIEWRYDDEETRRQLLNRALENAKKKAQQVADTLRVKLIGVYDFLE
                     TPMDDEAPRFQAQSAPVFRGAPAPAEQGLGMDIQHTKTLHMRVDIWYRVSELQD"
     gene            5218..6195
                     /locus_tag="PLE39_11460"
     CDS             5218..6195
                     /locus_tag="PLE39_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE68993.1"
                     /translation="MSPTINVQQLSKTYQVPEREGGFGAAVRSFFKRKYKDVKAVQQV
                     NFKIEQGEIVGFLGPNGAGKTTTLKMLSGLLHPTGGKAEVLGFTPWELKPEYLRVMTL
                     VMGQRNRLSWDIPAADSFLLNQAIYQIPDDEYKKTYKELDELLELEPLMKKPVRNLSL
                     GERMKCEIAAGLLHRPKILFLDEPTIGLDITGQARIREFLREYNKRTGATILLTSHYM
                     ADVTALCERIIIIHHGSLKYDGGINDLSKKIAPFKLIGVLLAESNSHDLSKYGEPVQN
                     EEDAEKQYIQVKAEDVTRVTSRLLADLPIHDITIADPPIEDVIEQAFNQ"
     gene            6273..7070
                     /locus_tag="PLE39_11465"
     CDS             6273..7070
                     /locus_tag="PLE39_11465"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNE68994.1"
                     /translation="MLLIFKRLWQVNWAEQWQYRANLVMYLLYWLVSPIIYLAVWTSI
                     ANAKGDVSGFTANDFITYYMLIIICDQLTSNIFIHIFAYKIQDGTLSGELTKPIHPVL
                     TNSLVVNIANKILNVIVLIPVWAILFLIFKPDFSSVTLQGILLSIPVMAVSFLIGYLL
                     SATITSLAFWTTRVYSIHEFYFALILLFSGQFVPLPLMPKVIQDIAQYLPFQLLLYFP
                     VQLILGKLNTNQIVQGYIMAGVWLAVSILLFNWVWRNGVKRFSAVGA"
     gene            7074..7859
                     /locus_tag="PLE39_11470"
     CDS             7074..7859
                     /locus_tag="PLE39_11470"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNE68995.1"
                     /translation="MQTLKLLSAFLKMNIQMSLAYRADTVVNIALNLMWLGWELLSLN
                     IIFNNTETLGGWGLGELIALLGVFRLVNTMMIAFIWPNTEKFNQSIRDGSMDYTILQP
                     VNSMFLVTFSRITVWRFWDLILAVVLIVVGINMSGDTTSALQILTFILLTITGMLVIY
                     SLWIILIAMTFWFTKFDNNVTILQALLDAGRYPATVYPVWLRVIVTFIIPIAVATTVP
                     LQALRGDLTLVNILIFTAIGFISFGIASQVWKAGLKRYSGASS"
     gene            complement(7895..8641)
                     /locus_tag="PLE39_11475"
     CDS             complement(7895..8641)
                     /locus_tag="PLE39_11475"
                     /inference="COORDINATES: protein motif:HMM:NF019359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNE68996.1"
                     /translation="MFTVKQLSTLAGVTPRTLHHYDEIGLLKPSRIGENGYRYYGEDA
                     LLKLQQILFYRELDIPLDEIKKIMGRRDFDVLGALQSHKDALSKQAVRINRLIQTVDN
                     TIQHLKGKTKMSEEGLFEGFSEEVQEKYAREAEQMYDPETVRESNRKWKGYSAAKKEA
                     ILAEGKAVYMDMVAAMPKGAASAEVQAIVERWRKHMDYFWTPSLDQLPGLADGYSSDP
                     RFKANFDKMHPNLAEFMREAVKVYVEKHSK"
     gene            8724..9323
                     /locus_tag="PLE39_11480"
     CDS             8724..9323
                     /locus_tag="PLE39_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE68997.1"
                     /translation="MESWLTSTPYPGMILWIVLYISDYYMTLNSAKGFQEIGHFHFEG
                     SFELTPQFQEDINKQRRISRRHIIFLVVYTLVILVAWWLFAKTLKAEWLYSIYLGTLI
                     LMEVAVHLRHFRNLHMVKAIKNEGGVEGDITYQKKLSYRISANEFQTFAWLFFIVAVI
                     TYSPFFLGGVISCFGISFRHNAYAKRAAGTPKAVHADHS"
     gene            9307..9960
                     /locus_tag="PLE39_11485"
     CDS             9307..9960
                     /locus_tag="PLE39_11485"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNE68998.1"
                     /translation="MQIIHKDEDILVINKPADLPVLPDGWDKDAPYLVKMLEADFPKV
                     WVVHRIDKSTSGIIIFALTAEAHRSLNIQFEKHLVEKTYHAILNGSPKWLDKITKFPL
                     RANVGNKHRTVVDNRNGVRAETRFRILKQNQLSALAEAQPMTGRTHQIRVHAYALGYP
                     LLGDILYSAPETDIISRAALHAYSLSFDHPTSNERLTFTAPYPADFEKALQLLKLAS"
     gene            complement(10022..>10234)
                     /locus_tag="PLE39_11490"
     CDS             complement(10022..>10234)
                     /locus_tag="PLE39_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNE68999.1"
                     /translation="GGAEKVDYVHGEDVVERLSLQAGNAGFYLAGMHKSDLFKTVILD
                     GALPRKTFSMGEAREKRFYMEARKIS"
     assembly_gap    10235..10334
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10335..11122)
                     /locus_tag="PLE39_11495"
     CDS             complement(<10335..11122)
                     /locus_tag="PLE39_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNE69000.1"
                     /translation="MKIISDIGIQIPQVYLPKPGTDLKKWAVIACDQFTSEPEYWHEV
                     EKIVGDAPSTLNLTFPEVHLEKEGGDQRIQNIQSAMRKYMDDGILQPRDGLIYVERTV
                     AGKTRKGVILCLDLERYDYTKGSSSLIRATEGTIVDRLPPRIKIREGAAMELPHILVL
                     IDDPNHTVIEPLSAAKSKLEKLYDFDLMLDSGHLAGYAVSEEFENKVVEALRGLAKPE
                     TFTAKYDIGKDQPVLLFAMGDGNHSLATAKAIWEKMKPQVGMDHP"
     gene            complement(11169..12068)
                     /locus_tag="PLE39_11500"
     CDS             complement(11169..12068)
                     /locus_tag="PLE39_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HNE69001.1"
                     /translation="MKILICDKTEQEYIEQMRAAGLTVDVRDDITPEELPNVLPAYDG
                     MVVRSRTKVRQALIDVCPNLKVIVRGGAGLDTIDHEYAKSKGIAVMNTPLANSKSVAE
                     LAIGYMLMMARSLYKASATMKAEKWDKKSFDGDEIGGKTLGLIGIGNIGKEVARRAIA
                     MDMTVVAYDPYVKEVEGIKLVSLDELLAQSDYISLHLPKTKESTGMIGAGQFAKMKKG
                     VRIVNCARGGIVDENALYDALTSGQVGAAALDVFAEEPPVDWKLMKLDNVIASPHIGA
                     ATKEAQARVGAEVADKLIAFSKK"
     gene            12347..13438
                     /gene="serC"
                     /locus_tag="PLE39_11505"
     CDS             12347..13438
                     /gene="serC"
                     /locus_tag="PLE39_11505"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNE69002.1"
                     /translation="MSDLKRAYNFNPGPGALPLEVLQQAQAELLDFKGTGMSVMEISH
                     RSKEFEAVIQTAEADLRELLGIPSNYKVMFLQGGATLQFTMLAMNLRPAGASADYIVT
                     GTWSKTAIKEAKKLGAARAAANTEADGFNCTPASLDLDPKAAYLHFTSNETIHGVEFF
                     TEPTPPDGVPLVCDTSSNFISRPIDVSKYAFLYAGAQKNAGPSGVTVVIARDDMIERT
                     PENLPVMLDYKTLAASGSLHNTPPSFAIYMVGLVFQWAKKQGGLAAIEKTNRIKAGLV
                     YDAIDQSGGFYRGHAKKEDRSLMNIPFRLPSEELEETFAKEAKKNNLIGLKGHRSVGG
                     MRASVYNAVTVEGAKALVDFMKEFQKKNG"
     gene            complement(13564..14121)
                     /locus_tag="PLE39_11510"
     CDS             complement(13564..14121)
                     /locus_tag="PLE39_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69003.1"
                     /translation="MKNTFAILFVFVLSACATVSISSSPEPPVSIETSPALTAEVPET
                     QVTAQSIYTSTKYGYSLSYPGTLNVVVVSDEYVEIGEKITVEVMNVDPTAPRGDGAVI
                     ETISDTQVSGYPAKLLTGYIGSVGGYIPQQFRKVVVERNGSYFVVTLYALGLHVTDGD
                     LSKIAELAMVDISLFDNVTASMQIP"
     gene            complement(14371..14886)
                     /locus_tag="PLE39_11515"
     CDS             complement(14371..14886)
                     /locus_tag="PLE39_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69004.1"
                     /translation="MILPKKRDPRFITIRRGGTLQDIDHQLLALWAADCAQHVLYLFE
                     ETQLGDDRPRRAIEAARAWAQGKITMSQSKAAGGHAMAAARNLRGASRYAAYAAGQAG
                     VVAHVAAHELGAAAYAIKAVREAAPDGQVEEFGHLEWKWQCAQLPNRIRDLVLNDQEL
                     RNEICWFMFSH"
     gene            complement(14922..15278)
                     /locus_tag="PLE39_11520"
     CDS             complement(14922..15278)
                     /locus_tag="PLE39_11520"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNE69005.1"
                     /translation="MTERAEITDEFIQKRIMTGRQYCVRLYKVGPNRSQPQDEVEGIQ
                     KEHLRYLWQLRAEGKLLISGPVMDDSDLQGVGIFQSTDKEEVKKLCEGDPAVKAGRLV
                     YEIYSWFGLPGDSLPE"
     gene            complement(15457..>16098)
                     /locus_tag="PLE39_11525"
     CDS             complement(15457..>16098)
                     /locus_tag="PLE39_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HNE69006.1"
                     /translation="EKAAAGLWSTPTDLAKFAIEIMNSCNNRSNKMLSKNMADSMLTT
                     IQGDYGLGLVVKHLENNIIRFGHSGGNVGFRCYFAAYTGSGKGAVVMTNSDNGNFVAM
                     EIIRSLSRIYEWSDFQPMEKQIDRQVGSDVYSQYVGKYQHPEYSDFFVQIEKDGSGLF
                     IEAIPGAPRWELYPESETRYFCVETETSIEFQKNNDGVFDALIYFSMELKKVV"
     assembly_gap    16099..16198
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16199..16723)
                     /locus_tag="PLE39_11530"
     CDS             complement(<16199..16723)
                     /locus_tag="PLE39_11530"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNE69007.1"
                     /translation="MSNENIQAVENGLLAAVSIHGVPEKKHTLMDRMKYFCVPGLSIA
                     VINNGEIEWARSYGVLEAGKNQAVSSDTLFQSASISKPVSAMMALKLVEQDRLNLDQD
                     VNEILSSWKIPENEFTDTNKVTLRGILSHTAGLTVHGFQGYDFDGEVPSLLQMLDGLP
                     PANSDPIRVDVRPGS"
     gene            complement(16940..17563)
                     /locus_tag="PLE39_11535"
     CDS             complement(16940..17563)
                     /locus_tag="PLE39_11535"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE69008.1"
                     /translation="MSRFGSDPLSFFNSVYQNIAPWDIGTPQPAMAALIEKHPPANPV
                     LDLGCGSGDLAIYLAKLGNQVVGIDFVESAIKNAQDKVKSLPDETAQIIKFQVADALK
                     PSLLQDKFGAVFDSGFYHLFNPDQCEQLIDEVASILKPHGCYYLHEFAVEFSIPNVPR
                     QISIDELQTRFTVEKGWRIKEVQTVEFLSRVAPPVPAICACVERLPM"
     gene            complement(17655..18806)
                     /gene="chrA"
                     /locus_tag="PLE39_11540"
     CDS             complement(17655..18806)
                     /gene="chrA"
                     /locus_tag="PLE39_11540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNE69009.1"
                     /translation="MTDITKSDISQESYWQLFLRFLRFGFLAWGGPVAQIAMIRQELV
                     DEEKWISKERFNRVLAVYQVLPGPEAHELCVYFGMIARGRIGGLLAGLGFMLPGFVLM
                     FALSWVYVTYGINSPIFAAVFYGCKAAVVALIIRAIHRIGGHSLTDYWLLGIAIAVGL
                     ASLLGVNFLFTLSLAGFAYSVIKRQSPSSVLYAFLPIPLFAQSAGILAPSLFALFGYG
                     LRSGLLTFGGAYTVISFLQYDAVVTGSWMTNAQFLDGLALSGILPAPLIIFATFVGYL
                     GGGAIGAIALTIGIFLPAFGFTLIGHEYVERLVENKSAHAFLDGVTAGVVGLIVVTAI
                     GLFREAVFDIPTIIIFALALLAVYRWKAKAAVVGIMFGAGLLGWLLFGV"
     gene            complement(18993..19922)
                     /locus_tag="PLE39_11545"
     CDS             complement(18993..19922)
                     /locus_tag="PLE39_11545"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNE69010.1"
                     /translation="MQPDNASSPNIYINEDLVGQLIASQFSKWANLPLKPVEVSGWDN
                     RTFRLGNDMSVRLPSEAWYVAQVEKEQYWLPKLAPFLPVQIPIPLALGYPDKNYPWHW
                     SIYKWLEGENASIENIKNLHEFAISLAQFLVALHQIDSSGGPPPGEHNFFRGGALKVY
                     DDETRSSIFALRNEINFDAAAEVWEISLDTTWNKEPVWLHGDFASGNLLVNKGQLSAV
                     IDFGCLGIGDPACDLTITWTFLSGESRNAFIKTLALDYATWARARGWALWKALITLVE
                     HIEEDDNKSKHARYVINEVLEDHKYRSDTFKRI"
BASE COUNT         5233 a         5193 c         4559 g         4817 t
ORIGIN      
        1 tccccgctga aaaacggctt gtttttgctg tctaatcggc tatactacga atacctttat
       61 cattttcagc actcgaaacg agaccgtcat ggataaaacc atccttgaaa tcccaaccga
      121 ccttttggaa gcggcaaaac tgacagccga cgaggcaaaa acggaactgg ccatccgcct
      181 ttatcaattg cacaaactca acgacgaaca ggctggcgta ctggcaggca acccaaagct
      241 catcgaagaa atggcgtgga acaatcgcag cacaggccgc ttcgatatgg acgaattcct
      301 ctcctgggcc tcgcacgacc tgaaaacccc gctcaacgcc atcatcggct tcacccgtgt
      361 ggtgatgaaa ggcatggacg gccccgtcaa cgacatccaa accaccgacc tcaccacggt
      421 ctacaacggc ggtcaacgca tgctggcgct gatcggcaac ctggtggata tggcccgtct
      481 caacaacagc gagatcaccc tcaaccgcga accctgcgaa ttgcgcagca tcctcgtcaa
      541 cacagctgac cagtggcagg tcaagaatcc caacaaaaac ctcacaaccg atatcatcat
      601 ccacgaaccc gtttacaaca tagacctggc gcgcgtcaac cagatcgtca ccagcctgct
      661 cagcctcgcc gccacccgcg tcgtcgaagg gtcgctctcc ctctccgccc gcgacaacga
      721 caacggcgcg gtcatcaccg ttcaaagcac aggccgaaaa tcacgcgaca agttcgaaat
      781 ggattcagcc atgctgggtt tcatcagttc ctcgctgatc aaattgcacg gcggaaatac
      841 agaatcggtc gaagagaccg aagacggcat gatgatcgtt ttccacctgc cgcgctgact
      901 catccacgcc tccgcgtcat gacgtataat cacgtcaact caacgcggag aatgcaggaa
      961 tgtctcaaat ctacgattat gtgatcattg gctcggggtt cgggggaagt gtttcagcaa
     1021 tgcgattggc cgaaaagggg tattcggtcc tcgttctcga aaaaggcaaa cgcttcaacg
     1081 ataaagattt cgcaacctcc aactggcaat attggaaata tgtctggatt cccgccctgc
     1141 gtgcgcatgg cattttgcag atcgccatcc tcaaaggtgt gatggtgctg cacggcgcag
     1201 gtgtgggcgg cggaagtttg ggctacgcca atgtgctcga agtgcccaac gaagagacct
     1261 tcgccacccc cgcctggaat caaaacatca aatggggcga tgtcctgcgt ccgcactatg
     1321 aaacggcgaa gaaaatgctt ggcgcagcac gcaaccccaa attatggaaa gccgacctgc
     1381 tgttgaaaga ctttgcagaa gaacgcggca tggggcacac cttccgcgcc acagatgtcg
     1441 gcgcgttttt tggtgaggca ggcgttaccg tgcccgatcc attcttcggc ggagaaggtc
     1501 ccaaccgcgc tgggtgccag cactgcggtg gatgcatggt gggatgccgc cacaacgcca
     1561 aaaacaccct gccgaaaaat tatttgtatt tcgccgagaa aaaaggcgcg caagtcaaag
     1621 ccgagctcga agtgatggat gtaaaaccgc ttccaactgc gctggaaact ggtgcccgat
     1681 acgaagtgca ttatcgcccc tccacttccc tgctcaaatc gcaacacatc gtccacgcca
     1741 gaaatgtgat cttctccgca ggtgtgatgg ggaccatgaa attgctgctg aatctgcgcg
     1801 acgtgaagaa atcactgccc aaactttcgg cgagactcgg cacaatggtc aggaccaact
     1861 cagagggctt gttgggaagc gtggcacgca gatccgatat caactactcg gaaggcgtat
     1921 ccatttcatc catctacaac catgatgaaa tcacccgcat cgaaccagtg cgctaccccg
     1981 atggctcgtc gttgatgcgt ttcctcgccg caccgttgat cgacaacaac gccagcatcc
     2041 ccatgcggat cataaaattt ttaggctggg cgctcaccca ccccattgat tttgcaaaag
     2101 cagcttttct gccgggctgg gcgcacaatg tgaccatttt gctggtgatg caaaacgccg
     2161 acaatcgaat gcgctttcga atgggacgca gcctgttcac ccttttccgc actggtctgg
     2221 tcgctgatga agaacctggg ttcaagatca acgcgcaggt gaaaggctca cacgagatca
     2281 cacgcgaatt cgccaaacgg acgaatggtg tggcgctcgg ctcgatcggt gaaaatctgt
     2341 tgggcctgcc caccaccgcg cacatccttg gcggcgcacc gatcggaaaa tcccccgaag
     2401 agggggttgt taatgaaaat ttcgctgtgc ataattatcc cggcttatac atcattgatg
     2461 gttcgatcat gcctgccaac ccgggcgtga acccttcgct gacaattacc gcattggccg
     2521 aatacgtcat gagcaaggtg ccgccccgca gcgcaaccac tgactgaaca agccaaaaca
     2581 atttttgagg agatctcttt taaatgcctg aatcgatcat ggaatattgg aagtttgacg
     2641 aagccgacct cggtgcgaac cgtctggggc aattaaccga aaagcagaag aaatatcttg
     2701 tcggcgaaca caaatcgcag cgcagtgtgt ttcttggggt gggcggcgtg gtcgccttcc
     2761 tgttcttatg cctgccagcg ctgttgatcg ggtcgagagt ggtattgccc atgctgctca
     2821 acggcgatct ctcagacttg caggacattc tgcccctggc tgccatgggt ggtttcggca
     2881 ttgtttttac cggtattgca gtgttaaccg ttggggctgt ggtggctatt tacgtcatgc
     2941 gcgccagcaa acccgccgaa ataaccgtca caaccgcaag aggcaaagtg gaatacactt
     3001 ggggcacaaa acgcgtcagc acaccgggca gcagtgtccg cagttatgaa gatgtaaaag
     3061 tgctgcactt gaatctgggc gataaaaaat tcgaagtgaa ggaaagcctg caagagctca
     3121 tcaaagaagg cgaagactgg tcggtttact acacttcgta tccgttcaag tttttatcgg
     3181 ctgaaaagaa ataaatgccc atgagttttt ccaccgcgcg cgaattcgcc cagcaattgg
     3241 atacccacga caggctggcc gccattcgca acgaattcgt catccacgac ccaaacctga
     3301 tctatctcga cggcaactca ctcggcctgc tgcccaaagc ggcacaggcc aaagcccgcc
     3361 aagttgtgga agaacaatgg ggcgtggatt tgatccgcag gtggaacaaa ggctggtggg
     3421 attctcctgc gcgtgtgggc gacaagatcg gtcaactcat cggcgcagca gcagggcagg
     3481 ttttggtcaa cgacacggtc tcactcaatt tgttcaaact cgccgccgcc gcgctcacct
     3541 tgcagcccag caagacccgc atcatcaccg acactttcaa tttcccatct gacctgtata
     3601 ttttgcaggg catcaggcag acctttggcg gccgccacga aatcatctcc atcggcgcaa
     3661 gcgacaacga catcaccccc gaccttgcgg cgcttgaaac tgcaattgac gaaaacacag
     3721 cgctggtcac actttcgcat gtcaccttca aaagcggcta tctctacgac atgcagcgca
     3781 ttacagaact cgcccataaa aaaggcgccc tcgtcctttg ggacctgagt cactccgtcg
     3841 gcgcgctgcc cattcacctc gattcctgca atgccgatct tgccatcggc tgcacctaca
     3901 aatatctcaa cggcggccct ggcgcacccg catttctcta cgtcaacaaa tccattcagg
     3961 aaaaactatc ctcgcccatc tggggctggt gggggcaaaa caatccgttc gatttcagtc
     4021 tcaattacca acccgcccgc ggcgcagaac gttttctggt cggcacacaa cccatccttt
     4081 ccctgcttac catggaagcc gcccttgaac ccaccctgca agcaggcatg gactccatcc
     4141 gcgaaaaatc cattttgatg acaggctacg cctctttctt aaccgaaagc tggctcgccc
     4201 ccttcggctt ttcgctcggc tctccgcctg accctgccaa acgcggatcg cacatcagca
     4261 tccgccacgc agatgggtat cgcatcaacc gcgccctcat cgaagaaatg aacgtcatcc
     4321 cagatttccg cgaacccgat aacatccgcc tcggttttgc acccctgtac acctcattca
     4381 ccgatgtatg ggaaggcttt gagcggatca aacgtgtgat ggaagaaaag cgccacgaaa
     4441 aatattcgaa gcaaaaattg accgtaactt aacacaggca caaagagaaa caccttcttt
     4501 gaaacgcaaa ggagaaaaat gaccaccaaa cccgacacca tcaagatctc catcatggag
     4561 aaagaggaaa tctccgccac acatgcagat ttgcacgtga ccatcaaagg cacatcgctg
     4621 gtcagcggga gcgaagcgat gaaaaaagcc aaggaggtga atcagcttgt tgaagcactg
     4681 accagcctcg ggcttaagag cgaagcgttc ttccttcagg gtgtccatct tgaacaggca
     4741 ggcggcgctc tgttaaagtc atccagcgcg gtgtatcgac tcaagatccg ctgtgaagat
     4801 ctggaaaaac tgtccgcgat cctcgatggc atctccgcgc aaaagaacgc cacgctcgaa
     4861 cgaatcgaat ggcgatacga tgacgaagaa acccgccgac aactgctgaa tcgcgcgctt
     4921 gaaaatgcaa agaaaaaggc ccaacaggtg gcggataccc tgagagtgaa attgatcggg
     4981 gtttatgatt ttcttgaaac accgatggac gatgaagcac cgcggtttca agcgcaatcg
     5041 gctcccgtgt ttcgtggagc gccagccccc gccgaacaag gcttgggaat ggacatccaa
     5101 cacacgaaaa ccctgcacat gcgcgtggac atttggtatc gggtttcaga acttcaagac
     5161 tagctctttt gttctactaa tgataaaatc cttcctcccc acaaaggaag gatttttatg
     5221 tccccaacga tcaacgtcca acaacttagc aagacctatc aagtgcctga gcgcgaaggt
     5281 ggttttggcg cggcagtccg tagttttttc aaacgcaaat acaaggatgt aaaagcggtt
     5341 caacaggtga atttcaagat cgagcaggga gagatcgtcg gtttccttgg cccgaacggc
     5401 gcaggaaaaa ccaccacgct caagatgctt tctggtttac tgcaccccac tggtggaaaa
     5461 gccgaagtgc tggggttcac cccctgggaa ctcaagcctg aatatctgcg ggtgatgacc
     5521 cttgtgatgg gtcaacgcaa ccgcctttcg tgggatatcc ccgcggcgga ttcgttcctg
     5581 ctcaatcagg ccatttacca aatccctgac gatgaataca aaaagacata caaggaactg
     5641 gatgaactgc ttgaacttga gccgttgatg aagaaacccg tgcgcaacct ttcgcttggt
     5701 gaacgcatga aatgcgagat cgccgcagga ctgctccacc gcccgaaaat tttgtttctg
     5761 gatgaaccca ccatcggtct cgacatcacg gggcaggcac gcatccgcga gtttctgcgt
     5821 gagtacaaca aacgcacagg cgcaaccatt ttgctgacca gtcattacat ggcagatgtg
     5881 accgcgcttt gtgagcgcat catcatcatt catcatggca gcctcaaata tgatggcggc
     5941 atcaatgacc tctccaaaaa gatcgcccca ttcaaattga tcggggtatt gctggcagaa
     6001 tccaattcgc acgacctttc aaaatatggc gaacccgttc aaaacgaaga agatgccgag
     6061 aagcaatata tccaggtcaa ggctgaggat gtgacgcggg tcacttccag gctgttggca
     6121 gatctgccga tccacgacat taccatcgcc gatccgccca ttgaggatgt gatcgagcag
     6181 gcattcaatc agtagaccat ctgtcattgc aggaaggctt caacctgacg agcgccttct
     6241 gggcgcatcg tgcaatgaag aaaggaaaat ccatgttact catcttcaaa cgtctctggc
     6301 aggtaaactg ggcggagcag tggcaatacc gcgccaacct tgtgatgtac ctgctttact
     6361 ggctggtctc ccccattatt tacctcgccg tatggacgag cattgccaat gcaaagggag
     6421 atgtaagcgg ctttacggcc aacgacttca tcacctacta catgctgatc atcatctgcg
     6481 accagctcac cagcaacatc ttcattcaca tcttcgccta caaaattcag gacggcacac
     6541 tttcaggtga attgaccaag ccaatccacc ctgttctgac caacagcctg gtggtcaata
     6601 ttgcaaacaa gatcttgaat gtcatcgtcc tgatccccgt atgggccatt ctgtttctga
     6661 ttttcaagcc cgacttcagc agcgtcactc tgcagggaat tttgctgtcc attccagtca
     6721 tggcggtttc ttttctgatc gggtacctgc tttctgccac catcacctcc ctcgcatttt
     6781 ggacgacacg cgtgtactcc attcacgagt tctactttgc cctcatcctg cttttctcgg
     6841 gtcaatttgt gccgcttccg ctcatgccaa aggtgatcca ggatatcgcc caatatttgc
     6901 cgtttcagtt gttgctttat tttcccgtcc aacttattct cggaaagctc aacaccaacc
     6961 agatcgttca gggatacatc atggcaggcg tgtggctggc ggtctccatt cttcttttca
     7021 actgggtctg gcgcaatggc gtcaagcgtt tttcagccgt gggggcataa aacatgcaaa
     7081 ccctcaagct tttatctgca tttctaaaaa tgaacatcca aatgtcgctg gcttataggg
     7141 cggatacggt tgtgaatatt gcgctcaacc tgatgtggct cggctgggaa ttacttagcc
     7201 ttaacatcat tttcaacaac acagaaaccc tcggcggttg gggccttggt gaattgatcg
     7261 cactgttggg tgtctttcgg cttgtcaaca ccatgatgat cgcgttcatc tggccaaaca
     7321 cggaaaaatt caaccagagc atccgcgatg gctccatgga ttacaccatc ctccagccag
     7381 tcaacagcat gtttctggtc actttttcac gcatcaccgt ctggcgtttc tgggatctca
     7441 ttctggcggt cgtgctgatc gtggtgggga tcaacatgag cggagacaca acatccgcgc
     7501 ttcagatcct gacctttatc ctgctcacca ttacaggcat gctcgtcatc tacagcctgt
     7561 ggatcattct gatcgcaatg accttctggt tcaccaaatt cgacaacaac gtcaccatcc
     7621 tgcaggcatt gctggatgca ggccgatatc ccgccacggt gtaccctgtc tggttaagag
     7681 tgatcgtcac gttcatcatc cccattgctg ttgcaacaac ggtgccgctg caagccctgc
     7741 gcggtgacct gacccttgtc aacatcctga tctttacggc catcgggttt atcagttttg
     7801 ggatcgcctc acaagtctgg aaggctggcc tcaaaagata cagcggcgcg agcagctaaa
     7861 aacaggcgac cagagattct ggtcgcctgt ttttttactt tgaatgcttt tcgacataca
     7921 ccttcacagc ctcgcgcata aactcagcga gattcgggtg catcttgtcg aaattggctt
     7981 tgaagcgtgg gtcagaggag tagccatcag ccaggccagg caattgatca aggctcggcg
     8041 tccagaagta gtccatgtgt ttacgccatc gctcgacaat ggcttgcact tcagccgagg
     8101 ctgcgccttt tggcatcgca gcgaccatgt ccatgtaaac ggctttgcct tctgccagaa
     8161 tggcttcctt tttcgccgcg gaatacccct tccacttgcg atttgattcg cgtacggtct
     8221 cggggtcgta catttgctcg gcttctctgg cgtacttctc ctgcacttct tcactaaagc
     8281 cttcgaaaag tccttcttcg ctcattttcg tttttccttt caaatgttga atcgtattat
     8341 ccacagtttg gatcaggcga tttatccgaa ccgcctgttt gctcaaggca tccttgtggc
     8401 tttgcagcgc gccaagcaca tcaaaatcac ggcgtcccat gatctttttg atctcgtcca
     8461 gtggaatatc cagttcgcga tagaacagga tctgctgtaa cttcaacagg gcatcctccc
     8521 cgtagtagcg atagccattc tcgccaattc gggatggctt gaggagcccg atctcgtcgt
     8581 agtggtgcag cgtccgcgga gtgacgccag ccagagtgga aagctgcttg actgtgaaca
     8641 tgtcgctact ataaaccctg acgttacgtt aatgtcaagg attttttgga tagcaggtac
     8701 aataagccaa acggagtatt tttatggaat cctggctcac atccacccct tacccaggca
     8761 tgattctatg gatcgtgctc tacatcagcg attattacat gaccctgaat tccgcaaagg
     8821 gatttcagga aatcggtcat tttcatttcg aaggcagctt cgaactcacg ccgcaatttc
     8881 aagaggatat taataagcaa agaagaatct caagaaggca catcattttc ctggtggtat
     8941 acacgctggt cattttggtg gcctggtggt tgttcgccaa aacgctcaag gctgaatggc
     9001 tgtactccat ctatcttggc acgctgatcc tgatggaagt ggccgtacac ttgcgtcatt
     9061 tccgcaattt gcacatggtg aaagccataa aaaacgaagg cggagtggaa ggtgatatca
     9121 cctatcagaa aaaactctcc taccgaattt cagccaacga atttcaaacc tttgcatggc
     9181 tattttttat tgtagccgtc attacctata gtccattttt ccttggcggg gttatctctt
     9241 gttttgggat cagtttcaga cacaacgcct acgcaaagcg cgcggcggga acaccaaagg
     9301 ctgtacatgc agatcattca taaagacgaa gacatacttg taatcaataa acccgccgac
     9361 cttcctgtat tgcccgatgg ttgggataag gatgctccct atcttgtaaa aatgcttgaa
     9421 gcggattttc caaaagtctg ggtcgtgcat cgtattgata aaagcaccag cggcatcatc
     9481 atctttgcat tgactgccga agcgcaccgc tcgctgaaca tccaatttga aaaacacctt
     9541 gtagaaaaaa cctatcatgc cattctgaac ggctcgccaa aatggctcga caagatcacc
     9601 aaatttccgc tgcgcgccaa tgtgggcaac aaacaccgaa ccgttgtgga taatcgaaat
     9661 ggcgtccgtg ctgagacgag attcagaatc ttgaaacaga atcaactttc ggctttggca
     9721 gaggcgcagc cgatgacggg gcgaacgcat cagatcagag tccatgccta cgcgttgggc
     9781 tatccgcttt tgggagacat cctctacagc gcacccgaaa ctgatatcat ctcacgcgcc
     9841 gcattacacg cgtattcatt atcgtttgat catccaacat caaatgaacg gcttactttt
     9901 accgccccct accctgctga tttcgaaaaa gcgttgcaac ttttgaagtt ggcaagttaa
     9961 aaagtttgca ggttgacagg tattaacttg tcaacctgca acattcaact tccaacgcga
    10021 actacgaaat ttttcttgct tccatgtaga agcgtttttc acgcgcctca cccattgaaa
    10081 aagtttttct cggcaacgcg ccatcgagga tcacggtctt gaacagatcg gatttgtgca
    10141 tgcccgcaag ataaaagcct gcattgcccg cctgcaaaga caggcgctcg acaacatctt
    10201 caccgtgaac ataatccact ttttcggcgc cgccnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnggatga tccatgccga cctgcggctt catcttttcc caaatggctt
    10381 tggccgtggc aagcgagtga ttgccatcac ccatcgcgaa aaggagtacg ggctgatctt
    10441 tgccgatgtc atacttggcg gtgaaggttt caggcttggc aagtccgcgc aaagcctcga
    10501 cgactttatt ttcgaattcc tcgctgacgg cataccctgc aagatgacca ctatcaagca
    10561 tgagatcgaa atcatacagt ttttcaagtt tcgacttcgc ggcagacaac ggctcgatga
    10621 cagtgtgatt cgggtcatcg atcaacacga ggatgtgcgg cagttccatt gcggcgcctt
    10681 cgcggatctt gatgcgcgga ggaaggcgat ctacgatggt gccttcggtt gcgcggatca
    10741 ggctcgatga gcctttggtg taatcgtagc gttcaaggtc aaggcagagg atcacgccct
    10801 tgcgagtttt tcccgccacg gtacgttcaa catagatcaa gccatcacgc ggctggagga
    10861 taccgtcgtc catgtatttg cgcatggcgg attgaatgtt ttgaatgcgc tggtcgccgc
    10921 cttctttttc gagatgcact tcggggaagg tcaggtttag cgtggaagga gcatcaccga
    10981 cgattttttc cacttcatgc caatactcgg gctcggaggt gaattgatcg caggcgatca
    11041 ctgcccactt tttcaagtcg gtgcctggtt tggggagata cacttgcgga atttgaatgc
    11101 cgatgtcgga aatgattttc atgtcttctc ctcagtaagg gatacggcga cgccgtatcc
    11161 ctacaagatt atttcttcga aaacgcgatc aacttgtcgg caacttccgc gccgacgcgg
    11221 gcctgggctt ctttggtggc tgcgccaatg tgcggggatg cgatgacgtt atcgagcttc
    11281 atcagcttcc aatcaacggg cggttcttcg gcgaagacat ccagcgcggc cgctccaacc
    11341 tgaccactgg tcaatgcgtc gtagagagcg ttctcatcga cgattccacc gcgcgcgcaa
    11401 ttgacaatgc ggacaccctt cttcatcttc gcaaattgac ctgcgccaat catacctgtg
    11461 gattctttgg tcttgggcag gtgcaggctg atgtagtcag actgggcaag cagttcatcg
    11521 agggagacca gtttgatgcc ttccacttcc ttgacgtagg gatcataggc gaccacagtc
    11581 atatccatgg cgatggcgcg gcgggcaact tccttgccga tgttgccaat accgatcagg
    11641 ccgagggtct tgccgccgat ctcatcaccg tcaaaggatt ttttatccca tttttcggct
    11701 ttcatggtgg cgctggcttt gtagagtgaa cgcgccatca tcaacatgta accgatggcc
    11761 agctcagcca cagacttgga atttgccagc ggggtgttca taaccgcaat gcccttggat
    11821 ttggcatatt catggtcaat ggtatcgagg cccgcgccac cacggacaat cactttgagg
    11881 ttggggcaga catcgatcag tgcctggcgc actttcgtgc gtgaacgcac aaccatgcca
    11941 tcataagcgg gcaatacatt gggcagttct tcgggggtga tgtcgtcgcg cacatccacg
    12001 gtgaggcctg cggcacgcat ttgctcgata tattcctgtt cggtcttgtc gcaaattaag
    12061 attttcatgg gtttctcctt gattggggca gaaattaata acaggaaccc aaccgacgaa
    12121 tcggctgaat tcctgtttgg aaggttactt tcaacacgct ggtcattcta acaacgagaa
    12181 aaacctttgt caacgacata catcagtagg gaatcatgcc gattgttctt gtagatttgt
    12241 ctgacaacca tttataatga agtgaatgta tcagaaaaac cgaaccagtc caacggtcaa
    12301 ataagaaccg taaaaaacct tccacatagt gaacaggagt aaccttatgt ctgatctcaa
    12361 acgtgcgtac aatttcaacc ccggtcctgg cgcgttgccg cttgaagtgt tgcagcaagc
    12421 acaggcagaa ttgctcgatt tcaaaggtac gggcatgtcg gtgatggaaa tcagccatcg
    12481 ctcaaaggaa tttgaagcgg tcatccagac tgcagaggcg gatctgcgtg aactgctggg
    12541 cattccatcg aattacaagg tgatgttcct gcaaggcggc gccactttgc aatttacgat
    12601 gctggcgatg aacctgcgcc ctgcgggtgc ctctgccgat tacattgtga caggcacctg
    12661 gagcaagacc gcgatcaagg aagcgaagaa gctcggtgcc gcgcgtgccg ctgccaatac
    12721 tgaagcggac ggtttcaact gcacccccgc aagccttgac cttgacccga aggccgcata
    12781 tttgcacttt acctccaacg aaaccattca cggcgtggaa tttttcaccg aacccacccc
    12841 gcccgatggc gttccgctgg tttgtgatac ttcatccaat ttcatcagcc gccccattga
    12901 tgtatcgaaa tatgcctttc tgtatgctgg cgcacagaaa aacgctggcc cctctggcgt
    12961 aaccgtggtc attgcccgtg atgacatgat cgagcgcacc cccgaaaacc tgcccgtgat
    13021 gctggattac aaaactctcg ctgcaagcgg atcactgcac aacacaccgc ccagtttcgc
    13081 gatctacatg gttggtctcg tctttcagtg ggctaaaaaa caaggcggcc tcgctgccat
    13141 tgaaaaaacc aatcgcatca aggctggtct ggtttacgat gccattgacc aaagcggcgg
    13201 cttttatcgc ggccacgcca agaaggaaga ccgttcattg atgaatatcc ccttccggct
    13261 acccagcgaa gaactcgaag agacctttgc aaaggaagcc aagaagaaca acctgatcgg
    13321 cctcaagggt catcggtctg tcggcggtat gcgtgcatct gtttacaatg ctgtaaccgt
    13381 tgaaggcgcc aaggcattgg tcgatttcat gaaagagttt caaaagaaaa acggataata
    13441 aaacaaggaa acaataaatt aaaaagcctt tgagcgaaaa ctcaaaggct tttttgaatt
    13501 tttggcaagg ccttcgacac caaaaactct tccccagcga gagtggagca ttcgaagatg
    13561 gaactagggt atctgcatgg atgccgttac attatcgaac aaagaaatat ctaccatcgc
    13621 aagttctgcg attttcgaaa gatcgccgtc tgtaacatgt aagccaagcg cataaagagt
    13681 gacgacaaaa tagctgccat ttcgttcaac tacaaccttc cgaaactgtt gagggatata
    13741 accaccaaca gagcctatat accccgttaa taatttagca ggataacccg aaacttgggt
    13801 atcggagatg gtttcaataa cggctccatc tccgcgaggc gcagtcgggt caacattcat
    13861 tacctcaact gtaatttttt cgccaatttc aacatattca tcgcttacaa cgacgacatt
    13921 cagagtacct ggataactca gagaatatcc atattttgta ctggtataga tgctttgtgc
    13981 agtaacctgg gtttcaggta cttcagcagt aagagcagga gaagtttcaa ttgaaactgg
    14041 cggttcaggc gaggaggaaa tggacacagt agcacaggct gatagaacga atacaaacaa
    14101 gatcgcaaac gtatttttca taagttcctt tttttaatat ttgaaaatta ttgaccattg
    14161 cgcgattcta attgaatgac ggatttgata aagtccccca atggtctata ttatctacat
    14221 tgaaacagat acgattcaga agcaattcac tgtaaaagct ccattttgag ggggaaatca
    14281 aatatttatc catcctaaga cagcgaaaca agagcacttt cgaaccgaaa tttcgcaact
    14341 cccatatgaa ccctatggga caaggttttc ctaatgacta aacatgaacc aacagatttc
    14401 attgcgcaat tcctggtcat taagcaccaa gtcacggatc ctattcggga gctgcgcgca
    14461 ctgccatttc cactcaagat ggccgaattc ttcaacctga ccatctgggg ctgcttcgcg
    14521 tactgcctta atcgcatagg cggccgcccc caattcatgc gccgccacat gagcaactac
    14581 acccgcctga ccagcagcat atgcagcata gcgagatgct cctcttaaat ttctggcagc
    14641 tgccatcgca tgaccacccg ctgctttgga ttgagacatt gtaatcttgc cttgcgccca
    14701 agcgcgcgca gcttcaatgg ctcgacgcgg acgatcatcc ccaagctgag tttcttcaaa
    14761 cagatacaaa acatgttgtg cgcagtcggc tgcccatagc gcaagcaatt gatggtctat
    14821 atcctgaaga gtgccgccac ggcgtatcgt gataaatcta gggtcgcgtt ttttgggcaa
    14881 tatcattctt taatatcctg tattttcctc tgcccggaga gttattctgg caaagaatcg
    14941 ccgggcagac caaaccagga gtagatttca tataccaacc gacccgcttt aactgccggg
    15001 tctccttcac acagtttttt tacctcctct ttatctgtgg attggaaaat acctacacct
    15061 tgaaggtcag agtcatccat aacaggtcca ctaattaaca attttccttc ggcccgaagt
    15121 tgccacaagt atcgaagatg ttccttttgg atgccttcca cttcatcttg aggctgacta
    15181 cgatttggcc ctactttgta taagcggaca caatattgtc ttcctgtcat gatccttttt
    15241 tggatgaact catctgtgat ttcagctctt tctgtcattc gcctgcaacc tttctctaca
    15301 agagtctcat ttgtcccata cgggtactta atacaaacca ccaatagcga gtcttactgg
    15361 tgagggttag caaaaagtta cgggtagaat ctgaccaagg gtacaaaatc ctctttcagg
    15421 aggtaaagac cgcactgctt cgtcgggaaa ttccaatcag acaacctttt tcaactccat
    15481 tgagaaataa atcaaggcat caaaaacgcc atcattgttt ttctggaact caatacttgt
    15541 ttcggtctca acgcaaaaat acctggtttc cgattcggga taaagttccc agcgtggcgc
    15601 acctggaatt gcctcaataa acaagcctga tccatccttt tctatttgaa cgaaaaagtc
    15661 agaatactca ggatgttggt actttcccac gtactgagaa taaacatcag aaccaacctg
    15721 acggtcaatt tgtttttcca taggctggaa atccgaccac tcatagatgc gtgagaggct
    15781 gcgaataatt tccattgcta caaaattgcc gttgtcacta tttgtcatca caacggctcc
    15841 cttgccgctg cctgtgtagg ccgcgaagta gcaacggaaa ccaacattcc caccactatg
    15901 accaaaacga atgatattat tttccaaatg ctttaccacc aaacccaatc cataatctcc
    15961 ctggatcgtc gtaagcatgc tgtcagccat attttttgat aacatcttat tcgaacggtt
    16021 gttacaagaa ttcatgatct caatggcaaa ttttgccagg tctgtgggcg ttgaccataa
    16081 accggcggct gctttttcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
    16201 tcccaggtct aacatccaca cgtatcggat ctgaattagc cggtggcagc ccatcaagca
    16261 tttgcaacaa tgatggtact tcaccatcaa aatcataacc ttgaaagccg tgaactgtta
    16321 gcccagcagt gtgtgaaaga atgccgcgga gagttacctt gtttgtgtct gtaaactcat
    16381 tctctgggat cttccatgaa gaaagaattt catttacgtc ttgatctaaa ttcaatctgt
    16441 cctgctctac aagtttgagc gccatcattg ccgaaacagg cttactaatt gaggctgact
    16501 ggaacaatgt atcggatgaa actgcctgat tcttacccgc ctccaacaca ccatagctgc
    16561 gcgcccactc aatctcccca ttgtttatca cggcaatgct tagaccagga acacagaaat
    16621 atttcattct atccatcagg gtgtgttttt tttcgggaac cccatgaatg gaaaccgccg
    16681 ccaacaaacc attctcaaca gcttgaatat tttcatttga catgtctatc accttttcta
    16741 ttggttctgc atgctttgtc ttcaaacaac gcttttattt cactcactca tgggaattaa
    16801 cttctgcatg aaaatattgt gagagtacgc gaagccgctc aacggtttgc gttatctgtg
    16861 ctgggacgga gacggcaaag ccatccaatc aaaaaatgat acggcataga agccccgtgc
    16921 atgttttgtc aggtgtggtt tacattggaa ggcgctctac acaagcgcag atcgctggca
    16981 cgggaggcgc gacccgacta aggaattcca ccgtttgaac ttccttaatg cgccaacctt
    17041 tttcaacagt gaaacgtgtt tgtaattcgt caatcgaaat ttggcgaggc acattaggta
    17101 tagagaattc gactgcgaac tcatgcaaat aataacaacc atgcggtttc aagattgagg
    17161 caacttcatc aattaattgt tcgcattggt ctggattaaa taaatgatag aaacctgaat
    17221 caaaaaccgc tccaaattta tcttgaagta aagatggctt tagggcgtct gccacctgaa
    17281 actttattat ttgggccgtc tcatcgggaa gggacttaac tttatcctgg gcattcttga
    17341 tggcagactc aacaaaatca atcccaacaa cttgattacc cagttttgcc aaataaatgg
    17401 caaggtcacc agagccgcaa ccgagatcca aaactgggtt tgcgggcggg tgtttttcaa
    17461 taagagcagc cattgcgggt tgaggcgtac caatatccca aggcgcaata ttctggtaaa
    17521 cagaattgaa aaaagaaagt gggtcagaac caaatctact caaaacaaat ctccttgtat
    17581 gttttcgtta agcgccttac ggcaatccaa agggtcagac gcatgcttcc tcggcacact
    17641 tttgacctca aaactcacac gccaaacaat agccaaccca acaagcctgc accaaacata
    17701 atacctacaa ctgcggcttt tgctttccaa cgataaaccg ctaataacgc cagcgcaaaa
    17761 ataatgattg ttggtatatc aaaaacggct tctcgaaaca gtccaattgc tgtaacaacg
    17821 ataagcccta ctactcctgc ggtgacgcca tctaaaaatg catgggcaga tttgttttct
    17881 acgagccttt caacatattc gtgaccaata agagtaaaac caaaagcagg taaaaatatt
    17941 ccaattgtta acgcaattgc gccaattgcg ccgccgccaa gataaccgac aaaagtagcg
    18001 aagataataa gcggagcggg aagaatgccc gacaacgcca atccatctaa aaattgagcg
    18061 ttggtcatcc agctgccagt tacaactgcg tcatattgca agaacgaaat gactgtgtat
    18121 gcaccgccga aagtcaataa accgcttcgg agaccataac cgaacaatgc gaatagtgaa
    18181 ggggcgagta ttccagcact ttgggcgaat aatggtatgg gtaaaaaagc atataagaca
    18241 gatgacggcg attgacgctt gatgaccgaa taagcaaacc cagccagaga aagggtgaaa
    18301 aggaaattga cgcctagcaa actggcaagt cctacggcga tagcaatacc caaaagccaa
    18361 tagtcagtta aagaatgtcc accgatgcga tgaatggcgc gaatgatgag ggctacaact
    18421 gcggctttac atccataaaa tacggcggcg aaaataggtg agttaatgcc gtaagtaaca
    18481 tacacccagg atagtgcaaa catcaacaca aagccgggga gcataaaccc aagccctgcc
    18541 aacaaaccgc caatacgtcc ccttgctatc atgccgaaat aaacacaaag ttcatgggct
    18601 tcgggtccag gcaaaacttg ataaacggct aaaacacgat tgaatctttc ttttgaaatc
    18661 cacttttctt catcaactaa ttcctggcga atcatagcaa tttgggcgac gggaccaccc
    18721 caagccaaaa aaccgaagcg cagaaaacgg aggaaaagtt gccagtaaga ttcttgcgaa
    18781 atgtcagatt tggttatgtc agtcattgag tatcctattt taacaataaa atgagattgt
    18841 tatgcaaggg cgtgccaacg gcatgcgtta cctgcgctgg ggcggagacg gaaaagccgt
    18901 ccagccagaa agctgcttgg gatatgcaca tgcctatggg aagtccctag catcaggtaa
    18961 atactttatt aggcgttttt tattttttag actcatatcc ttttgaaggt atcacttctg
    19021 tatttgtggt cctcaagcac ttcattgatt acatatctgg catgttttga tttattatca
    19081 tcttcttcga tatgttcaac aagtgtaatt aacgccttcc aaagtgccca accacgagca
    19141 cgcgcccaag ttgcgtaatc tagagcaaga gttttgataa acgcatttcg actttctcct
    19201 gacaaaaacg tccatgtgat agtcaaatca catgctggat cgccaattcc caaacatcca
    19261 aaatctataa ctgcgcttaa ctgtccttta tttaccagta aatttcctga tgcaaaatca
    19321 ccatgcaacc aaacgggttc cttattccat gttgtgtcaa gggatatttc ccaaacttcg
    19381 gcagccgcat cgaagtttat ttcatttctt agagcgaaaa ttgaactgcg agtttcatca
    19441 tcatacacct tgagtgctcc gccacgaaaa aagttatgtt ctccaggcgg cggaccgcca
    19501 gacgaatcaa tttgatgaag tgctacaagg aattgagcaa gtgaaattgc aaactcatga
    19561 agatttttaa tattttcaat cgaagcattc tcgccttcaa gccatttata gattgaccag
    19621 tgccacggat aatttttatc aggatagcca agtgccaatg gaatagggat ttgaactggg
    19681 agaaaaggtg caagttttgg caaccaatat tgttcttttt ccacttgcgc aacataccat
    19741 gcttcactgg gaagccttac actcatatcg ttaccaagac gaaaggttcg attatcccat
    19801 ccactaacct caactggctt aagtggaagg tttgcccatt ttgaaaactg cgatgcaatc
    19861 aattgaccta caaggtcttc gttaatataa atattaggag aagatgcatt gtcaggctgc
    19921 atatgtactc catatcattt tggaaggaaa tgcccaacgg gttgcgttac ttggttggcg
    19981 aatccgtccg actggaaaaa tg
//
LOCUS       DAOQJC010000069        19910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1238_length_19910_cov_10.045228, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000069 DAOQJC010000000
VERSION     DAOQJC010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 19910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19910
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1238_length_19910_cov_10.045228"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..2160)
                     /gene="fusA"
                     /locus_tag="PLE39_11550"
     CDS             complement(85..2160)
                     /gene="fusA"
                     /locus_tag="PLE39_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNE69011.1"
                     /translation="MARVHPLEKYRNIGIIAHIDAGKTTTTERIMFYTGMTHRIGSVD
                     DGTTVTDWMVQERERGITIVSAAVSAEWKGYQVNIIDTPGHIDFTAEVQRSLRVLDGG
                     VVVFDAVQGVEPQSETVWRQADRYGVPRICFVNKMDRVGASYERTIETIIDRLGANPI
                     PMQIPIGFESTFKGVVDLLSMKAIVWEDDLGKEPHVVEIPADLKDQAVEARAKMVEKI
                     AELDDDLTMKFLDAQEISIEELKVALRKGVLSTKAAPVFCGSSLKNKGVQVLLDAVVD
                     YLPSPADRPVITVSEPGNPDNTFDIPAQDDAPLGALVFKIVTDPYVGRLAYVRVYSGV
                     LNQGQTVQNSTKKGRKERIGRLIRMHADHREDVTEIRAGDIGAVLGFKESFTGDTLCD
                     TKALVLETISFPEPVISIAVEPKSSADQEKMGEALRKLAEEDPTLHVNSDEDSGQTIL
                     RGMGELHLDIVVDRLQREFRVPANVGAPRVAFRESITKPIKEVNYKYAKQSGGRGQYG
                     HVVFSMEPGERGSGIVFENKIVGGAVPKEYIGPIEKGVKEAADGGVLAGYPVVDLKVT
                     LIDGSYHEVDSNEMAFKMAAIMGFKEGVQKGNPILLEPMMKVEVVVPEEYLGDVMGQI
                     NSRRGLIQGMEVRPGNAQAVKAMVPLAEMFGYATQLRSATQGRGVFNMEFDHYAPVSQ
                     SVAEEILKA"
     gene            complement(2211..2678)
                     /gene="rpsG"
                     /locus_tag="PLE39_11555"
     CDS             complement(2211..2678)
                     /gene="rpsG"
                     /locus_tag="PLE39_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNE69012.1"
                     /translation="MRRAKPEKREILPDSRFNSVNLQTLVQHVLKRGKKSTAVRLIYD
                     AMDLIKERTEKNPMDVFDGALKNVGPAMEVRPRRVGGATYQVPMEVSADRRTTLAIRW
                     LLAAARDRSGKSFSDKLASELIDAFNETGSAIRKRDETHKMAEANRAFSHYRV"
     gene            complement(2703..3119)
                     /gene="rpsL"
                     /locus_tag="PLE39_11560"
     CDS             complement(2703..3119)
                     /gene="rpsL"
                     /locus_tag="PLE39_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNE69013.1"
                     /translation="MPTVNQMVRKGRKNNKTKSKAPALQFTLNSFKQRRVRQDKGAPQ
                     KRGVCTVVRTMTPKKPNSALRKIARVRLTNGIEVTAYIPGEGHQLQEHSVVLVRGGRV
                     KDLPGVRYHIVRGSLDTTGVANRKQGRSKYGAKRPK"
     gene            complement(3312..7211)
                     /locus_tag="PLE39_11565"
     CDS             complement(3312..7211)
                     /locus_tag="PLE39_11565"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNE69014.1"
                     /translation="MASSLPQKTYARIPVKLDLPNLIEVQLDSFERLKKEGLNDLFHE
                     ISPIESYNKGMKLFFPSRSPESQQWGLKYWFGDPKHGIEECVERDLTYASPLYVSVLL
                     AGPDVPEPIKQDIFLGDFPEMTDKGTFIVNGTERVVVSQLIRSPGVYFEAPADRATGR
                     PLAMAKLIPDRGAWMEFETRKSDYIILKFNRKRTVPITVFLRALAAVDDGLKDSPIKF
                     GTDEEILSIFKDVDNNPERMFIASSLKQEPDWSQYNQTIPNAALIEFFKKMRPGDPAT
                     LDNARQFLEEQLFDQRHYDLERVGRYKLNQKLDLNIPIPHRTVTKSDVIRLIRRMIQI
                     NNGAERPDDIDHLGNRRVKTVGELIQNKLRIGLRRMERVIKERMSIRDQEQLSPVTLV
                     NIRPVVAALREFFGSSQLSQFMDQTNPLAELRHKRTLSALGPGGLRRERAGFDVRDVH
                     HSHYGRICPIETPEGPNIGLIGRLASFARVNEYGFIETPYRRVYKTFKADDERLEGRT
                     LREDVYDPKSEELLFKSGTSIDAKALKKLAKVGGEISVVPFVSDEISYLSADAEDKYT
                     IAQANAQLTENKEFSRERISCRYHSGFLFAGSEAIDYMDVAPHQVVGISAALIPFLEH
                     DDANRALMGSNMMAQAVPLVRPEIPLVSTGMEGHAALDSGQVVVADADGEVVSVTGST
                     ITVKEKRSGNRVYQLRKYQRSNQSTCIDQRPSVVKGQLIKAGDIIADSSSTDSGQLAL
                     GQNVVVAFLSWEGGNFEDAILLSERLVQDDRFTSVHIEKHEVEARDTKLGPEEITRDI
                     PNVGDDAIKDLDENGIIRIGAEVGPNDILVGKITPKGEKELTPEERLLRAIFGEKSRD
                     VKDTSLRMPHGERGKVVDVKVFTREDNSDLSAGVDMMVRVSVAQRRKITAGDKMAGRH
                     GNKGVVSKVVPVEDMPFLEDGTPVDLILNPLGVPGRMNIGQVLEVHLGWAAKRMGFRA
                     ITPVFDGASEEQIEAELARAWIMDQSWKEAAEKAWEWLKDQEYDPNSIQDDDEVRRLY
                     LEHWLGKRKYDVYSLNDLDYARHATAREWLREKGYTNPEEILLEDRDVANPDPEIDDM
                     TVKACLRLWMESLGLTRRVAEDKVYDTAQEVSKEKGVPLPILGKQVLRDGKTGLPYDQ
                     PVTVGVMTILKLHHLVEDKVHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAY
                     GAAHTLQEMLTVKSDDVQGRVNTYEAIVKGEPIEEPSIPASFRVLVKELQSLGLAVEA
                     INEGGDVVKFGKDDEKAHPPKHDTGLLSLGENRTGKK"
     gene            7414..7486
                     /locus_tag="PLE39_11570"
     tRNA            7414..7486
                     /locus_tag="PLE39_11570"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7447..7449,aa:Phe,seq:gaa)
     gene            7514..7585
                     /locus_tag="PLE39_11575"
     tRNA            7514..7585
                     /locus_tag="PLE39_11575"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7546..7548,aa:Gly,seq:gcc)
     gene            7612..7682
                     /locus_tag="PLE39_11580"
     tRNA            7612..7682
                     /locus_tag="PLE39_11580"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7644..7646,aa:Cys,seq:gca)
     gene            7791..13805
                     /locus_tag="PLE39_11585"
     CDS             7791..13805
                     /locus_tag="PLE39_11585"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE69015.1"
                     /translation="MKNKQPFSALGISLIYAFIGGTWILLSDRLVTLIFTNPDQLSVI
                     QSYKGWSFVIVTAILLFILLKNRRNTLQAAEQNFSDLFESATEGIFRSSPDGRFLRVN
                     SAMAALYGYESPEDMLNLVHDISSQIHASSDGRRKFTEELTKNGYVKNYNAKNLRKDG
                     SIFWTSTNARVVKDANNQVLYYEGFVTDITQQKKGEQSLKEAEERYRTLVERLPSVIF
                     MDNFNDPQSTQYMSPRLKEILGYTPGEWEAGVNMWEKSLHPEDRERVLAEDARTDKTG
                     EPFRIEYRLRHRDGHYIWIKEDASIIKAEDGTPLFWQGTLLDITEQKNSEKVMKENEI
                     SYRELFNSVTQAIYIQDKEGVFLDVNDGAVKMYGYPREYFIGKTPEILSAPGKNDLES
                     LAQKIADTFNGTPQEFEFWGIRSNGQVFPKTVTLNKGTYFGKDVIIAIAQDITTRKQA
                     ESLLERQLKELTVLRATTMAGTQSNSEDDMIAQVTQITAEIYTDVCGILLLNEKGDTL
                     TPHPSYIGANVDKWKNGFPVTQGVSGRAVRSGRAIRIVDVTQDVDYIEIDSNTKSEIC
                     IPFRVQERIIGVFNVESRHKNAFDEKDERLLNTIAGGLGTAIEKLRLSKAEQVQHQRE
                     TALWELIRLANSSLDLNQVLQSILDQLQKVISSDAGSIQLLDKDRLVISAGIGSEILN
                     FSKKGPLLLGKYPLNNYVITEKKPVRLNNVYLDDRFKFFSENSQVLSFMGIPLIVKEK
                     VIGMITLESYQLNHFTKQDEELGLAVANHTSIAIENARLFNEEKRRTQIIETLANIAN
                     EIATTREVIPALDKIAERALSLLKASTLAIYLLQDDEKTIKIVTAKGAYQDQLLSHTL
                     EIGKGITGNIIATGRPEIVNDFSQDTRTVTVPGTPKEDARLDTMMSAPLTLHGKSFGA
                     INAWRPKVNGLFDNSELNFLVSIAHQASISIESVRLFEEITRHAQEAAAIAEVGRSIS
                     STLQLNKVLDSIAYYAKELLQAETSAVYLTDPNTPSLRAISALGRESDEIKNDPLKIG
                     NGILGNIALNKQGEIVNNTITDPRSIPVVGTEIERDEHIMGMPFLSKGQLTGLLVVWR
                     VGEDKAFKSTDLDFLNNLAQQATVAIENARLFEAEHKHLQNAEVLNQATSALANTLDV
                     YNLYENILDWLKQLAPYDSASIMMVNSNDTLKMTAMRNIPRHFSIGQIFPLTQKWKAI
                     IESRKPNILDDAQQNFDFQKWTGTEYIRSWMGVAMFIQDKLIGFINLDSKIVGTFTEE
                     HAVLVQTFANQAATALENARLFELEQKRRKESEIMRQATTVLSSLLDLPSLHEAILEW
                     LRKIVPYDSASIMEIEGAHIRITAAKGFQFPEKAIDHTFPIDHVLLKIMNETGEPLII
                     DDCNKDPRFERWGDAEKVRGWMGVPMITRGQVTGYITIDRVIPNTFAKNDALIAQTFA
                     HQAANSLENTRLYTETLQRLDELEMVSRVSFALRTAHDTREMIPILLNEIKANMGTDS
                     AAIWLYDHGKNELVPIATSGRLKDLPKSTFRPNEGIVGAVYSKGATHVSPELSRDILL
                     APENASFFGKGWSSVTLPIRTVSETIGVLVVAMSVPHKIGSNHIRLINTLTEIAGNAI
                     YRSNLFERSEEQIRRLTTLREMDTAITSSLDLHITLGILTEHLTTKMGVSAATVLVFN
                     PNSQTLNYHAPIGFKNREAVRGSQNIGDGLAGQILLNRRAIYIKDLNNEPDLPQMKIL
                     RTEQFVSYYAMPLFSKGATKGILETYFRHSFTPSGDWLDFIHTLAEQAIIAIDNAQLF
                     ENLQRSNQELSLAYDTTLEGWGKALELRDKETKGHTNRVTNLTLELARQMNIPETELL
                     HIRRGALLHDIGKMGVSDAILHKDGPLTQSELFEMRKHPKYAYDLLYPIAYLRPAVDI
                     AYCHHEWWDGSGYPRGLKGEEIPLAARIFAIIDVWDALLSDRPYRKAWSPKKTMKYIK
                     DLSNKQFDPTVVEAFSRMIENDTASKAANEPKKVLKKNPTSRTKKR"
     gene            complement(13834..15045)
                     /gene="tyrS"
                     /locus_tag="PLE39_11590"
     CDS             complement(13834..15045)
                     /gene="tyrS"
                     /locus_tag="PLE39_11590"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNE69016.1"
                     /translation="MNIEEQVELLMLGTEYGDQDLKKAMTAELRERLTLAQKENRPLR
                     VYCGYDPTSTDLHLGHTITMRKLRQFQDLGHEVTFLIGSYTALVGDPSDKNKARPILT
                     EEQVAENARTYTEQAFRVLDREKTKIRYNGEWLSKLSLVDLIRLGQNFTVQQFLARDN
                     FANRLEKGEPIYIHETFYALMQGYDAVAMETDVQIGGSDQLFNIIVAGRKLQEALGQK
                     PLVGIITAILPGTDGVQRMSKSTGNIVPINTGANDMFGKLMSVPDSAMGLYMRLVTRW
                     SPPEIEKIEQDVASGALHPRDAKMNMAGEITSIFYGEEDAKSARENFVKTFQQKEIPD
                     EMPEFSLQAGQTVVDVILAAGLAESKSKARSLIDQKGVKLDGEVLERGDAVFPHPGVL
                     QVGKRRFVRVK"
     gene            complement(15219..15896)
                     /locus_tag="PLE39_11595"
     CDS             complement(15219..15896)
                     /locus_tag="PLE39_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69017.1"
                     /translation="MKKMILVLAVLLTACGTSSQQHPVVVMPALMATAEPYIDPSYPT
                     AQVEVAAPNQTASGIEVRMDRVSMEGKNINADVCFTLPDTADWGIQSASLTYGGVVVQ
                     EFGTTLLSFQEPVNGAVGLRCDTLTFVVPPDADLTNAAIIIDAIGTTVRADDYCSIYM
                     PKIQQAMLDRGTGISVDCMDVNGMLTMQILSMPPGMTQEQAESIVYSDEFYTVKGPWT
                     FPFGLAQ"
     gene            complement(15916..16809)
                     /locus_tag="PLE39_11600"
     CDS             complement(15916..16809)
                     /locus_tag="PLE39_11600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNE69018.1"
                     /translation="MNIMIAGGSGFLGNALAKSFLMDGHKVFILTRGSRTMNGTQAIH
                     WDAKTTDGWGHWVNEMDVVIHLAGKTLASWPWTNAMKKAFHDSRIIPGLALVEAIQKA
                     TQRPGIFIQQSGINYYGLSGDLADESTPPGDDFLSQLAVQWENATKPLEELGLRRVIL
                     RTAPVLDKKEGLMPLMALPVKLFVGGPMGGGKFSAPWIHVQDWVGAVRHLIANEDARG
                     AYNLIAPEPTSNAEFNKTLADVLHRPYWFPVPAFLLRTFLGEMSVLVVEGRFSQPKRL
                     IESGYRYQFPGPREAMTDLFS"
     gene            16886..17389
                     /locus_tag="PLE39_11605"
     CDS             16886..17389
                     /locus_tag="PLE39_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNE69019.1"
                     /translation="MYEPVLVLNANFEPIHICSTRRAVGLILAGKAGLIVNGRGYIRT
                     ISQMLPRPSVIRLESQVHRPRPHVKLTRREIFRRDNYTCQYCGKRDGPLTVDHVIPRH
                     MGGQHIWTNLVAACSACNHRKGGRKVEEAHMHLLHIPKEPPTNAAYIFGRHLTENGEW
                     APYISGW"
     gene            complement(17371..18222)
                     /locus_tag="PLE39_11610"
     CDS             complement(17371..18222)
                     /locus_tag="PLE39_11610"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE69020.1"
                     /translation="MPKVIILGSSNAIPTKDSENTHMVLVGRERMVLIDSGSNPILRL
                     EQAGLDFNDLTDLILTHFHPDHASGIPLLLMDMWLLGREAPLNIYGLHYTLDRLESLM
                     GFYNWSDWPNFFPVIFHRLPLKEMTPVLNCSDFTIHASPVRHFIPNIGLRVEFAQSQK
                     VMAYSCDTEPCDEVVRLSENADVLIHEATGETPGHSSARQAGEVAKKAGAGRLYLIHY
                     PSGRYASGDPVAEAGENFRGTVALMKDFMVLKFGKRLPLSHFGRAAENSKNSSGKKAG
                     KIYQPEI"
     gene            complement(18457..>19910)
                     /locus_tag="PLE39_11615"
     CDS             complement(18457..>19910)
                     /locus_tag="PLE39_11615"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNE69021.1"
                     /translation="MPKEVTMFNKLRPVWLVAGRELKDQFRDWRVLTPMIILVIAFPF
                     LMNEFAKQTVDFLNQYEANLILDRLVPFSIMIIGFFPITISLVVALEAFVGEKERGTI
                     EPVLSAPLDDWQLYFGKLLVGVATPLTASYLSIALYLVMVVRQGLGIPSLSVMVQLLF
                     LTTAHATLMVSAAIVISVQSTSVKAANLLASFIVIPVAILMQGEAVMLFWGNEDVLWL
                     AVAGVMIISLLLIRVGIAHFQREYLLGREIDTINLRWIWGTFWKNFFGGAHSLREWYG
                     MSVGGSLRRVSSAVLVVTLIAIVSIWLGYDWMMVNFAPVIEHATPERLQKITNSAQDF
                     SGLTDLHRTITAPFLFANNTRAVAVIFLAGLVSFSVLGVLLYMVNIGLIGGLYALLKL
                     LGISPLPVFAAGILPHGIFELPAVMIGSAAMLYMGVALVTPQTGKSMGEVIIELFADW
                     LKIFLGLVVPLLAIAAVIEAYVTPVLLVNVMSK"
BASE COUNT         5532 a         5292 c         4341 g         4745 t
ORIGIN      
        1 ccatggtccc tacatttaga tgcggtttgc tgcgatcaaa tttttccttc gccataaaaa
       61 actccttgaa ttatgaaaaa acggttacgc ttttaaaatt tcttccgcca ctgactgcga
      121 tactggcgca tagtggtcaa attccatatt gaatacacca cgtccctggg ttgcagagcg
      181 aagctgtgtt gcataaccaa acatctctgc caacggaacc atggctttta cagcctgggc
      241 atttccagga cgaacttcca taccttggat caaaccacgg cggctgttga tctgccccat
      301 aacatcacca agatattctt ctggaacgac aacttcaacc ttcatcattg gttcaagcaa
      361 aatggggttt cctttttgta cgccttcctt gaaacccata attgcggcca tcttgaacgc
      421 catttcattt gagtcgactt cgtgatagga accatcaata agggtgactt tcaaatctac
      481 aacaggatat ccagcaagga caccaccatc tgcagcttcc ttaacaccct tctcgattgg
      541 tccaatgtat tccttgggaa cagcaccacc aacgatctta ttttcaaata caatgccgct
      601 tccacgctca cctggttcca tggaaaatac aacatgacca tactgaccgc ggccacctga
      661 ctgcttcgcg tatttgtaat tgacttcttt aattggtttg gttattgatt cgcggaacgc
      721 cacacgaggt gcaccaacat tggcaggcac tctaaattca cgctgcaagc ggtctaccac
      781 gatatccaaa tgaagttcgc ccatacctct aagaatggtt tggccagagt cttcatctga
      841 attaacatga agtgtcggat cttcttctgc caacttgcga agagcctccc ccatcttttc
      901 ctgatctgca gaactttttg gttcaacagc aattgaaatc acgggctcag ggaaagagat
      961 tgtttcgaga acaagcgcct ttgtatcaca aagggtatcg ccagtaaaac tttctttgaa
     1021 accaagaact gcaccaatgt cgcctgcacg aatctccgtc acatcttcac gatgatcagc
     1081 atgcatacga attaaacgtc caatacgttc ctttctgcct ttcttggtgg agttttgaac
     1141 ggtttgtccc tgatttaaaa cgccggaata aacacgaaca taagcaaggc gaccaacgta
     1201 cggatcggtt acaatcttaa acactaaagc gcctaaaggt gcatcatcct gagcaggaat
     1261 atcaaaggta ttgtcagggt tacccggttc agacacagtg ataacaggtc gatcagccgg
     1321 ggagggaagg taatcaacca ccgcatccag caaaacctgc acacctttat tcttcaaaga
     1381 agatccacaa aataccggag cagctttggt tgaaagaaca cccttacgaa gggcaacctt
     1441 cagttcttca atgctaatct cctgggcatc caagaacttc atggtcagat catcatcaag
     1501 ttctgcgatc ttttcaacca ttttggcacg ggcctcaaca gcctgatctt tcagatcagc
     1561 tggaatctca acgacatgag gttctttgcc aagatcgtct tcccaaacaa tagccttcat
     1621 ggaaagcagg tcaacaacgc ctttaaaagt tgattcgaaa ccaatgggga tttgcattgg
     1681 aatcgggttg gctccaaggc gatctataat ggtttctata gtgcgttcat aggaagcacc
     1741 gacgcgatcc attttattga caaaacaaat gcgaggtact ccataccgat ctgcctgacg
     1801 ccacacggtt tcagattgag gttcaacgcc ttgcacggca tcaaatacca ccaccccacc
     1861 atccaaaaca cgaagggaac gttgtacttc cgcagtaaag tcaatatgtc ctggggtatc
     1921 aataatgttg acttgatatc ccttccactc cgcggaaaca gcagcagaaa caatggtgat
     1981 accacgttcg cgttcctgaa ccatccagtc agttaccgtt gtaccatcgt caacagaacc
     2041 gatgcgatgg gtcataccgg tataaaacat aatacgctcg gtggtagtgg tcttcccagc
     2101 gtcaatgtgg gcaataatgc ctatatttct gtatttttcc agcggatgta cacgtgccat
     2161 tcttaactac ttcctattat ttagttttgc aaatttaaga ttaaatcaaa ttaaacacgg
     2221 taatgagaga aagcacgatt ggcttcagcc atcttgtggg tctcgtcacg tttacgaata
     2281 gcggaaccag tttcattaaa ggcatcaatt aattcagatg caagcttgtc agaaaaagat
     2341 ttaccagagc gatcgcgagc agcagcgaga agccagcgaa ttgccaaagt cgtgcgacga
     2401 tcagcagaaa cttccattgg cacctgatag gttgcaccac ccacacggcg tggacgaact
     2461 tccattgctg gaccgacgtt cttcaatgca ccatcaaaaa catccatggg gtttttttca
     2521 gtgcgttcct tgatcagatc cattgcatca taaatcaaac gaacagccgt gcttttctta
     2581 ccacgtttaa gcacatgctg aaccaaggtc tgcaaattta cgctattaaa acggctatcg
     2641 ggaaggattt cccgcttttc aggttttgct ctacgcatgc tctattactc catcttatat
     2701 tgttactttg gacgcttcgc gccgtacttg gaccggccct gcttacgatt cgcaacaccg
     2761 gtggtatcga gagaaccacg gacaatgtga taacgcacac ccggcagatc cttcacacga
     2821 ccaccgcgca ccaataccac tgagtgctcc tgcaactggt gaccttcgcc aggaatgtaa
     2881 gctgtgactt caataccatt ggtcaagcgt acacgagcaa tcttgcgcaa cgctgagttt
     2941 ggtttcttgg gcgtcatggt acgaacaaca gtacaaacac cgcgtttctg cggtgcacct
     3001 ttgtcctgtc gtacacggcg ctgcttgaaa ctgttcaatg tgaactgaag tgccggggcc
     3061 ttgctttttg ttttattatt tttgcggccc ttacggacca tttgatttac tgtcggcata
     3121 accgcctccg attacaaatt atttcacaaa ttcatttgaa tattcgcaat aaatgagacc
     3181 atcaacgaat ttttctgatg gcctcatttg tagctgccag ttgggtcgag tggggataaa
     3241 gccactcttg ctttcattgg gcaacggctg attagtttat cacgccatac cttttgcgtc
     3301 aataggaatc tctacttttt tccagttcga ttctctccga gactcaataa accagtatcg
     3361 tgttttggcg ggtgcgcttt ctcgtcatcc ttaccgaact tgaccacatc accaccttcg
     3421 ttaattgctt ccactgcaag accaagtgac tgcaattctt taaccagaac acggaaggag
     3481 gcgggaatgc ttggttcttc gatcggctca cctttcacaa tcgcctcata ggtgtttaca
     3541 cggccttgaa catcgtcaga cttgacagtg agcatttcct gaagggtatg tgcagcgccg
     3601 tatgcctcaa gcgcccaaac ttccatttca ccaaaacgct gaccaccaaa ctgtgcttta
     3661 ccacccagcg gctgctgagt aacaaggctg tatgggcctg tagagcgggc atgaacttta
     3721 tcttccacaa ggtggtggag tttcaagata gtcatcacac caacagtaac gggttgatcg
     3781 tatggaagac cggtcttgcc atcgcgcagc acctgcttac caagaatcgg aagtgggact
     3841 cctttttctt tggaaacttc ctgtgctgta tcatacactt tatcttcagc aacacgacgg
     3901 gtcaaaccaa gagactccat ccacaaacgc agacaggcct ttacagtcat gtcatcgatc
     3961 tcgggatcag ggttggctac atcgcggtct tcgagcagaa tttcttcggg gttggtatag
     4021 cccttttcgc gcaaccattc ccgggctgtc gcatgacgcg cataatccag atcatttaag
     4081 ctgtagacat catatttgcg tttaccaagc caatgctcga gatataagcg tcgaacttca
     4141 tcatcatctt gaatggaatt cgggtcatat tcctgatcct taagccattc ccatgctttt
     4201 tccgcagctt ccttccagga ctgatccatg atccatgcac gagcaagttc agcttcaatt
     4261 tgctcttcac tggcaccgtc aaacactggg gtaatagcac ggaatcccat acgtttcgcg
     4321 gcccatccca agtgaacttc caatacctga ccaatgttca tacgtccagg aacgccgagc
     4381 gggttcaaaa taagatcgac aggggttcca tcttcaagga aaggcatgtc ttcaacaggc
     4441 acaactttgg aaaccacacc tttgtttccg tgacgtcctg ccatcttgtc ccctgctgtg
     4501 atcttgcgac gctgcgcaac tgaaacacgc accatcatat ctacaccggc agagaggtcg
     4561 gaattgtctt cacgggtaaa tactttcaca tctacaactt taccgcgctc accatggggc
     4621 atgcgtagtg aagtatcttt cacatcacga gacttttcac caaagatcgc tcgcaacaaa
     4681 cgctcttcag gagtaagttc tttttcaccc ttaggtgtaa tcttcccaac aaggatgtca
     4741 ttaggaccta cctctgcccc aatgcgaata atgccgtttt catcaaggtc tttgatggca
     4801 tcatcaccaa cattgggaat gtcacgggtg atttcctcgg ggccaagttt ggtatcgcgg
     4861 gcctccacct catgtttttc aatatgaact gaggtaaagc ggtcatcctg aacgagacgt
     4921 tcagaaagta gaatagcatc ttcaaagtta ccaccttccc aggaaaggaa ggcgaccaca
     4981 acattttgac caagagccag ctgaccgcta tcggtagatg aggaatcagc aataatatca
     5041 ccagccttaa tcaattgtcc ctttacaaca gaaggacgtt gatcgataca ggtgctttga
     5101 ttagaccgct gatacttgcg aagctggtat acacggtttc cgcttctctt ttctttgacc
     5161 gtgatggtgg atccagttac cgagaccacc tcaccgtctg catcagcaac aacaacctga
     5221 cccgaatcaa gagccgcatg gccctccata cctgtagaaa caagtgggat ttcagggcga
     5281 accaaaggaa ctgcctgagc catcatgttc gaacccatga gcgcacgatt agcgtcgtca
     5341 tgttcaagga atgggataag cgccgcacta ataccaacga cctgatgcgg ggccacatcc
     5401 atataatcaa ttgcctctga gcctgcaaac aagaagccag agtgatatcg gcaggaaata
     5461 cgttcgcgtg aaaactcttt attttcggtc aattgagcat ttgcttgagc gatcgtatat
     5521 ttatcttcag catccgcaga aaggtaggaa atctcatcag agacgaaagg aacaacacta
     5581 atttctccgc caaccttggc aagcttcttc aaagccttcg cgtcaattga tgttccagac
     5641 ttgaagagga gctcctcgct ctttggatca tatacatctt cacgcagagt acgcccttcc
     5701 aagcgttcat catctgcctt aaaggtctta tacacccttc gatagggtgt ctcaatgaag
     5761 ccatattcat ttacacgagc aaaagatgcc aaacgcccaa taagaccaat atttggacct
     5821 tctggagttt caatcgggca gattctccca tagtgagaat gatgcacgtc acgaacatca
     5881 aaccctgcgc gttcacggcg aagaccacct ggacctaaag cagacaaagt tctcttatga
     5941 cgcaactcag ccaatggatt ggtttgatcc ataaactgtg ataattgaga agatccaaag
     6001 aattcacgca acgcagctac tacgggacgt atattgacaa gcgtcacagg agaaagctgt
     6061 tcctgatcac gaatggacat gcgttcttta ataacacgtt ccatgcggcg caagccaatg
     6121 cgtaatttat tctgaatcaa ctcccctact gtcttgacgc gacggtttcc caagtgatca
     6181 atatcatcgg ggcgttcagc accattatta atctgaatca ttcggcgaat caaacgaatc
     6241 acatcgctct tggttactgt acgatgtggg atgggaatgt tcaggtcgag tttttggttc
     6301 aacttgtaac gaccgacacg ttcaagatca tagtggcgtt gatcgaacaa ctgctcttca
     6361 aggaattgac gggcgttatc aagagtggca ggatcaccgg gccgcatttt tttgaaaaac
     6421 tcgattaggg ccgcattggg gattgtctgg ttgtactgac tccagtcagg ctcttgtttg
     6481 agcgaggagg caataaacat gcgctcagga ttgttatcta catccttaaa gatggagagg
     6541 atttcttcat cagtaccaaa cttaatgggc gagtccttca atccatcatc aacagcggcg
     6601 agggcacgca aaaatacggt gattggaact gtacgtttgc ggttgaattt caagatgatg
     6661 taatcggact tgcgtgtttc gaattccatc caagcgccac ggtctggaat caattttgcc
     6721 attgccagcg gacgccctgt tgcacgatca gcaggcgcct cgaaatatac gccaggagat
     6781 cgaatcaact gagatacaac aacacgttcc gtaccattta caatgaacgt gcccttatcg
     6841 gtcatctcag ggaagtcacc aaggaagata tcttgtttga ttggttcagg cacatctggg
     6901 ccagccaata aaacagagac atatagtggg ctagcgtacg tcaagtcacg ctcaacacat
     6961 tcctcaatcc catgtttggg gtctccaaac caatacttga gtccccattg ctgagactca
     7021 ggacttcggc ttggaaaaaa cagtttcatt cctttgttgt aggactcaat aggtgagatt
     7081 tcatgaaaca ggtcattcaa accctctttt ttgagtcgtt cgaatgaatc aagctgtact
     7141 tctattaagt tggggagatc tagttttact ggaatacgtg cgtaggtttt ttggggcaaa
     7201 gaagatgcca ttcttctctc ctaactctat ggctgggtta tgaaacgatt ttaactgtcc
     7261 cacttggaca gcgacctaac attatatcga aacgaatata aaaagtcaag aactatcatc
     7321 atcagcacag acataaaaca gattcgtgcg cgtttcttga caatctatat aagtacgtgt
     7381 aaaatcacga ccgctttaca aaaagatgtt catgccgaga tagctcagtt ggtagagcac
     7441 gcgactgaaa atcgcggtgt ccccagtccg atcctgggtc tcggcactct ggctgatggt
     7501 ttggccaaaa aacgcgggcg tagtacagtg gtagtacgtc tccttgccaa ggagaaggtc
     7561 gtgggttcga atcccatcgc ccgctcagat atagagaaat actatagaca tggcgtcgtg
     7621 gccaagtggt aaggcaaggg tctgcaaaat cctcatcagg ggttcaaatc ccctcgacgc
     7681 ctcttcaaaa gcaaatcact tctgatttgc tttttttgtt gcgaatttct catggagatt
     7741 ccatgaaaaa aagtctataa tcaaatatga gaaaaaatct gttcagaacc atgaagaaca
     7801 agcaaccatt ctctgcatta ggcatttctt taatatacgc ttttataggg ggcacatgga
     7861 ttttactatc cgaccgtctt gtaactctta tcttcacgaa tcctgatcaa ctatccgtta
     7921 tccaatctta caaagggtgg tcctttgtca ttgttaccgc aatcctgttg tttatattat
     7981 tgaaaaacag gagaaacaca cttcaagcag ctgaacaaaa cttctcagat ctttttgaat
     8041 cagcaacaga aggtattttc cggtcttcac cagacggacg ttttcttcgc gtcaattctg
     8101 caatggctgc cttgtatgga tatgaatcac cagaagacat gcttaatctt gtccatgaca
     8161 tcagttctca aattcatgcg tcttctgatg gtcggcgcaa gttcacagaa gaactgacaa
     8221 agaatggata tgtaaaaaac tacaatgcga agaatttacg aaaagatgga agcatcttct
     8281 ggacatcaac aaatgcccgc gtcgtcaaag acgcaaacaa ccaggttcta tactacgagg
     8341 ggttcgtaac tgatattaca caacaaaaaa agggggagca atctttaaaa gaagctgagg
     8401 agcgctaccg tactctggtc gaaagattac catccgtcat ttttatggat aatttcaatg
     8461 acccacaatc cactcaatac atgagtccgc gattgaagga aattcttggt tacacaccag
     8521 gggaatggga agccggggta aatatgtggg aaaaatcact gcaccccgaa gatagagaaa
     8581 gagttcttgc agaagatgcg cggactgaca aaactggaga acccttccgc atcgaatacc
     8641 ggctgcggca tcgagatgga cactacatat ggataaagga agatgcctcc atcatcaaag
     8701 cggaggatgg cactccccta ttctggcagg gaacccttct tgacattaca gaacaaaaaa
     8761 actctgaaaa ggtcatgaag gaaaatgaaa tcagttaccg agaacttttc aacagcgtaa
     8821 cacaggccat ttatattcag gataaagaag gagtatttct tgatgtaaac gatggcgcag
     8881 tgaagatgta tggataccct cgggagtatt ttattgggaa aacccccgag atattgagtg
     8941 cccccgggaa aaatgatttg gaaagccttg cgcaaaaaat cgcggatact ttcaatggaa
     9001 ctcctcaaga gtttgaattt tggggcatcc gaagtaatgg acaggtattc cctaaaaccg
     9061 taaccctcaa caaaggcacc tacttcggca aagatgtaat tattgccatt gcacaggata
     9121 ttacaacccg caaacaggcg gaaagtctgc ttgaacgcca actaaaagaa ttaaccgttt
     9181 tgcgcgccac caccatggct ggcacacaaa gcaattctga agacgacatg attgcccaag
     9241 tgacccagat caccgcagag atatatactg atgtatgtgg aatactcttg ctgaacgaaa
     9301 aaggggatac actcactccc cacccctctt atatcggcgc aaatgttgac aaatggaaaa
     9361 acggttttcc agtcacccaa ggcgtttcag gcagagcagt gcggagtgga agggccatac
     9421 gcattgtaga tgtcactcag gatgtggatt acattgaaat agattcaaac acaaaatctg
     9481 agatctgcat cccttttcga gtgcaggaac gtatcatcgg tgtatttaac gtggaaagca
     9541 ggcacaaaaa cgcatttgat gaaaaggatg aacgcctgct caacaccatc gcaggcggtc
     9601 tgggaaccgc aatcgaaaaa ttgcggcttt cgaaggcaga gcaagttcag catcagcgag
     9661 aaacagccct atgggaactt atccgcctgg ccaactcctc attggactta aatcaggtgc
     9721 tccaatccat tctcgatcaa ttacaaaagg tcatatcttc cgacgcaggc agcattcaac
     9781 tcctggataa agaccgtctg gtcatttctg ctggaatcgg atctgaaatc ctcaactttt
     9841 caaaaaaggg gccgctcctg cttggtaaat accccttgaa taattacgtc atcacggaaa
     9901 aaaagccagt ccgacttaac aacgtttacc ttgacgatcg cttcaaattt ttcagcgaaa
     9961 actcgcaggt gctctcattc atgggtattc cgttgattgt caaagaaaaa gtgattggca
    10021 tgatcacgct cgaaagctat caactcaacc acttcaccaa acaggatgag gaacttgggc
    10081 ttgcagtagc aaatcatact tccatcgcca ttgaaaatgc gcgcctattc aacgaagaaa
    10141 aaaggcgcac ccagatcatt gaaacattgg caaatattgc caacgaaatc gctaccacca
    10201 gagaagttat cccggcattg gataagatcg ccgaacgtgc cctttccctg cttaaagcca
    10261 gcacacttgc catttaccta ttgcaggatg acgaaaaaac aataaagatc gtaaccgcca
    10321 agggtgccta ccaggaccag ttgctttcac acacccttga aattggaaaa ggcatcacag
    10381 ggaatattat tgccacaggc aggcctgaaa tcgtaaatga cttttcgcag gatacgcgta
    10441 cagtcactgt tcccggtact cctaaagaag atgcccgcct tgacacgatg atgtctgctc
    10501 cactcacctt gcatggaaaa tcctttggcg ccatcaatgc gtggcggcca aaagtaaacg
    10561 gcttattcga taactcagaa ctgaacttcc ttgtcagtat cgcacatcag gcatccattt
    10621 cgattgagtc ggtacggctt tttgaagaaa tcacacgtca cgcacaggaa gcagctgcca
    10681 tcgccgaagt tggccgcagt atttcatcca ccttacagct caataaggta ctggacagca
    10741 tcgcctacta cgccaaagaa ctcctgcaag cagaaactag tgccgtgtat ttaaccgacc
    10801 ccaatacacc cagtcttaga gccatatcag ctcttggacg tgaatcagat gagatcaaga
    10861 atgatccgct gaaaatcgga aatgggattc tgggcaacat tgccttaaac aagcagggtg
    10921 agatcgtcaa taatacgatc acagacccgc gctccattcc agtggttgga actgaaatcg
    10981 aacgtgatga acacatcatg ggaatgccgt tcctttccaa gggacagctg accggcctgc
    11041 tggtggtttg gcgcgtcggg gaggacaagg cgttcaaatc caccgacctc gattttctca
    11101 acaatctcgc tcaacaagcc acagttgcca ttgaaaatgc acgcctgttc gaagccgaac
    11161 acaaacacct gcaaaacgca gaagttctaa atcaggctac atccgctctc gccaacacat
    11221 tggatgttta taacttatat gaaaacatcc ttgattggtt aaaacagctt gccccctacg
    11281 acagcgcatc cattatgatg gtcaattcaa acgacaccct caaaatgacc gccatgcgca
    11341 acatcccaag gcatttttcc atcgggcaga tctttccgct cacccaaaaa tggaaagcca
    11401 tcattgaaag ccgcaagcca aatattctgg atgacgccca acagaacttt gattttcaaa
    11461 aatggacagg taccgaatac atccgcagct ggatgggtgt agccatgttc attcaagata
    11521 aactgatcgg ctttataaat cttgacagca aaatagtggg cacatttacc gaagaacacg
    11581 ccgttttggt acaaactttc gccaatcagg cagcgaccgc acttgaaaac gcccgcctct
    11641 tcgaattgga acaaaaacgc cgcaaagagt ccgagatcat gcggcaggcc accacagtac
    11701 tctccagcct gctcgacctt ccatcccttc atgaagccat cctcgaatgg ctgcgcaaga
    11761 tcgtgccata cgacagtgcc tccattatgg aaatcgaagg agcgcacatc cgcatcactg
    11821 ccgcaaaggg attccaattc cctgaaaaag ccattgacca caccttcccg atcgatcatg
    11881 ttctattaaa gatcatgaat gaaacgggcg aacctttaat tattgacgac tgcaacaaag
    11941 acccgcgctt cgaacgctgg ggcgatgccg agaaagtccg cggatggatg ggcgttccca
    12001 tgatcacacg cggacaggta acaggctaca tcaccattga tcgtgtcatt ccaaacacgt
    12061 ttgccaaaaa cgatgccctc atcgcccaga cctttgccca tcaggcggcc aattctctcg
    12121 aaaatacacg cctctacacc gagaccctgc aaagactcga cgaactcgaa atggtcagcc
    12181 gtgtctcttt tgccctgcgc accgcacacg atacaagaga aatgatcccc attttgctca
    12241 acgagatcaa agccaacatg ggcacagact cggctgccat ctggctctac gatcacggca
    12301 agaacgaact cgtcccgatt gctacaagcg gccgattaaa agacctgccc aaatccacgt
    12361 tcagaccgaa cgagggaatt gtcggggcgg tatattccaa aggtgcaaca cacgtcagcc
    12421 ccgagctatc tcgcgatatc ctactcgccc ccgaaaatgc cagcttcttc ggaaaaggct
    12481 ggagcagtgt cacgcttccg atccgaactg tctctgaaac cattggcgtt cttgttgttg
    12541 ccatgtcggt accgcacaag atcggttcca atcacatccg cttgatcaat acactcactg
    12601 aaattgcagg aaatgccatt taccgttcca accttttcga aagaagcgag gaacagatcc
    12661 gccgcctcac tacactgcgc gaaatggata ccgccatcac ctccagcctc gacctgcaca
    12721 ttaccctcgg aattctgaca gaacacctca ccaccaaaat gggcgtcagc gccgccaccg
    12781 tactggtatt caaccccaac agccaaacgc tgaactacca cgcccccatc ggattcaaaa
    12841 atcgtgaagc cgtacgcggc tcacaaaaca tcggcgatgg ccttgcagga caaatcctct
    12901 taaatcggcg ggcgatctac ataaaggatc tcaacaacga gcctgacctg ccgcagatga
    12961 aaatcctgcg aaccgaacaa ttcgtaagtt attatgccat gcccctcttc agcaagggcg
    13021 ccaccaaggg catcctcgaa acctatttcc gccactcgtt tacccccagc ggagactggc
    13081 ttgatttcat ccacacactg gcagaacaag ccatcatcgc cattgacaat gcccaactct
    13141 tcgaaaacct gcagcgcagc aaccaggaac tctccctcgc atatgacaca acactggaag
    13201 gctggggcaa agcgctggaa ctgcgcgaca aagaaacaaa gggacacacg aaccgcgtca
    13261 caaacctgac cctcgaactg gcgcggcaaa tgaacattcc cgaaaccgaa ttattgcaca
    13321 tccgtcgcgg cgccctgctc catgatattg gcaaaatggg tgtatctgac gccattctcc
    13381 acaaggacgg cccgcttacc cagagcgaac tgttcgaaat gcggaaacat cccaaatacg
    13441 cctatgacct gctctacccc atcgcctacc tcaggcccgc agtggacata gcctactgcc
    13501 atcatgaatg gtgggatgga agcggatatc cacgcggcct caaaggagaa gagattcccc
    13561 ttgccgctcg aatctttgcc atcatcgacg tttgggatgc cttactctca gaccgccctt
    13621 accgcaaggc atggtctcca aaaaagacca tgaaatacat caaagaccta tcaaacaaac
    13681 aattcgaccc gaccgttgtg gaagccttca gccgcatgat cgaaaatgac acggcttcaa
    13741 aagcggccaa tgaacccaaa aaagtgctca aaaagaaccc aacaagcaga acaaaaaaga
    13801 gatgagctgg ctggctcatc tctttttata aatttactta acccttacaa atcgccgctt
    13861 ccccacctgc aacacaccag ggtgtggaaa tacagcatcg ccgcgttcca acacttcacc
    13921 atccaatttc acacccttct ggtcgatcaa ggagcgggcc ttgctcttgc tttcagccag
    13981 ccccgccgca aggatcacat ccaccaccgt ctggcctgcc tgcaaggaaa actcaggcat
    14041 ttcatcggga atttccttct gttggaaggt cttgacgaaa ttctcacgcg ccgacttcgc
    14101 gtcttcttcg ccatagaaaa tgctggtgat ctcacctgcc atattcatct tggcatcgcg
    14161 tggatgcaag gctcctgaag ccacatcctg ctcgatcttc tcgatctcgg gcgggctcca
    14221 gcgtgtgacc aaacgcatgt acaaccccat cgccgaatca ggcacagaca tcaatttacc
    14281 gaacatatcg ttcgcgcctg tattaatcgg cacgatattt ccagtggatt ttgacatccg
    14341 ctgcacacca tctgttcctg gcagaattgc agtgatgatg cccaccaacg gtttctgccc
    14401 cagcgcttcc tgcaatttgc gcccggcaac gatgatattg aataactggt ccgacccgcc
    14461 gatctgcaca tcggtttcca tggcaacagc gtcatacccc tgcatcaacg cataaaaggt
    14521 ctcatgaatg taaattggct cgcctttttc aaggcgattg gcaaaattat cgcgcgccaa
    14581 aaactgctga acggtgaagt tctgcccaag acgaatcaaa tccaccagcg aaagcttcga
    14641 aagccactca ccgttgtagc ggatcttcgt cttttcacgg tcaagcacac gaaaggcctg
    14701 ctcggtgtat gtccgcgcat tttcagcaac ctgctcctcg gtcagaatgg gacgcgcctt
    14761 gttcttatcc gaagggtcac ccaccagagc agtatagctc ccgatcagaa aagtgacttc
    14821 atgtcccagg tcttgaaact ggcgcaattt gcgcatggtg atggtatgtc ccaaatgcag
    14881 atcagtggat gttgggtcgt aaccacagta cacacgcaac ggcctgttct ctttttgggc
    14941 aagggtcagc cgctcgcgca attcagcggt catggccttc ttcaagtcct gatcgccata
    15001 ttcggtgccg agcatcaata actcaacctg ttcttcgatg ttcataactt ctccttcata
    15061 ccttaaccgt acattacggt gaattgacaa acaaaaacgc gcccgcgatg atgtgggcgc
    15121 gttcaaagca ctccatcgca gggattatgg atgggtgtaa taataatatt tgttgaacga
    15181 actggaactg gacatgcgcg tattatacat caagaatttt actgtgccaa accaaacggg
    15241 aaggtccacg ggcctttgac ggtgtagaac tcatcgctgt acacaatgga ttcggcctgc
    15301 tcctgggtca ttccgggggg catgctgagg atctgcatgg taagcattcc gttcacatcc
    15361 atgcaatcca ctgaaatacc ggtgccacgg tcgagcatgg cctgttggat cttgggcatg
    15421 taaatggagc agtaatcatc ggcgcgcacg gtggtgccga tcgcatcaat gatgatcgcc
    15481 gcattggtca ggtcggcatc gggcggcacc acaaaagtca acgtatcaca gcgcaatccc
    15541 accgccccat tgacgggctc ctgaaaactc agcagggttg tgccaaactc ttgcaccacc
    15601 actcccccat atgtgaggct cgccgactga atgccccaat cagcagtatc gggaagggta
    15661 aaacaaacat cagcattgat attttttccc tccatcgaaa cccggtccat acgcacttca
    15721 atgccgctgg cagtttggtt cggcgcagcc acttccactt gcgcagttgg gtatgaagga
    15781 tcaatatacg gttctgccgt ggccattaat gctggcataa ccaccacagg gtgctgttgt
    15841 gaagaagtac cacaggcagt caggaggact gccagcacaa ggatcatttt tttcatggat
    15901 ttctctcctt tgacattaac tgaataaatc ggtcatcgct tcacgcggac ctggaaactg
    15961 gtaccggtac cctgactcga tcagccgttt gggttgggaa aacctgcctt ccaccaccaa
    16021 gacgctcatt tcccccaaaa aggtcctcag taaaaatgca gggacaggaa accaatacgg
    16081 acggtgaagc acatcggcaa gcgttttatt gaattctgcg tttgaggtcg gctcgggcgc
    16141 gatcaggttg tatgccccac gggcatcttc atttgcgatc aaatgccgca cggctccaac
    16201 ccagtcttga acatggatcc acggcgcgga aaattttcca ccgcccatgg ggccacccac
    16261 aaataattta acaggcagag ccatcaacgg catgagccct tcttttttat cgagcactgg
    16321 cgccgtgcga aggatcaccc gccgcaatcc caactcttcg agtggcttcg tggcattttc
    16381 ccattgcacc gcaagctgcg aaaggaaatc atccccgggc ggcgtggatt cgtctgcgag
    16441 atcgccgctc aggccataat aattgatgcc cgactgctga ataaaaatgc ccgggcgctg
    16501 agtcgctttt tggattgctt caacaagcgc cagtccggga atgatgcgag agtcatgaaa
    16561 ggcttttttc atggcgttgg tccacggcca ggaggcaagc gttttcccgg cgaggtgaat
    16621 caccacatcc atctcgttga cccaatgccc ccagccgtct gtcgtttttg catcccagtg
    16681 gatcgcctgt gttccgttca tggtgcgcga accacgggtg agaataaaga ctttgtgccc
    16741 atccataaga aaggattttg cgagggcgtt acccaaaaaa cccgagccgc ccgcgatcat
    16801 gatgttcatt tcaaatgctc ctgtaatgcg ccgccgcgcg gattcattta agtggcagta
    16861 taatcatgca gattaccgag gcaaaatgta cgaaccggtg ctggtactca acgctaattt
    16921 tgagccgatc catatttgct caacacgcag agctgttgga ctgatacttg cagggaaagc
    16981 cggtttgatc gtcaatggac gcgggtacat ccgcacaatc tctcagatgc ttccccgccc
    17041 ttcggtcatt cggttggaat cacaagttca tcgtccgcgc cctcacgtta aattaacgcg
    17101 ccgcgaaata ttccgtcgcg ataattatac ctgtcagtat tgcgggaaac gagatgggcc
    17161 tcttactgtg gatcatgtca ttccacgcca catgggtggc cagcatattt ggacaaacct
    17221 ggtggcggcc tgctctgcct gtaatcaccg caaaggcggc cgcaaagtgg aggaagccca
    17281 catgcacctc ctgcatattc ccaaggaacc acccaccaat gccgcctaca tctttgggag
    17341 gcatctgacc gagaatggcg agtgggcgcc ttatatttcg ggctggtaga tttttcccgc
    17401 cttcttacct gaactgtttt tgctgttttc tgccgctctt ccgaaatggg agagcggtag
    17461 tcttttacca aatttcaaga ccatgaaatc tttcatcagg gctactgtgc cgcggaaatt
    17521 ttcaccggcc tctgcaaccg ggtcgccgct cgcataacgc ccgctgggat agtggatgag
    17581 atacagcctg cctgcacctg ccttctttgc cacctcaccc gcctgccttg cagaagaatg
    17641 acccggagtt tcacctgtgg cttcatgaat caacacatcg gcattttcac tcagacgcac
    17701 aacctcgtca cagggctcag tatcgcagga atacgccatc accttctggc tttgggcaaa
    17761 ttccacccgc aggccaatgt ttggaatgaa atgccgcaca ggcgaggcat ggatcgtaaa
    17821 atcggaacaa ttcaagaccg gggtcatttc cttgagcggc aggcgatgga agatgactgg
    17881 gaaaaaattc ggccaatcag accagttgta aaaacccatc aagctctcaa gacgatccaa
    17941 tgtgtagtgc aatccataaa tattgagcgg agcttcacgc cccaataacc acatatccat
    18001 caaaagcaat ggaatgcccg aagcatggtc agggtggaaa tgggtcagaa ttagatcggt
    18061 gaggtcatta aaatccagcc cagcctgctc aagtcgcaaa atcgggttgc tgccggaatc
    18121 gatcaacacc atccgctccc tgccgaccaa caccatgtgt gtattttcac tgtcttttgt
    18181 agggatcgca ttcgacgaac caaggatgat tacttttggc atatggttat cttatcctac
    18241 attcctgcct tcgtgaatca caattttatt tcgaaaattc gaaccgaacc ttctcaaatt
    18301 gtatcgcttg acggcagatt ttccagtcct tttcacataa tcaggcaaag ccgaaaagca
    18361 caaaaagcgc ctttccaact ttgcctgatc tgaaaatcaa tttttacaac gaagacttaa
    18421 tgcaaaacca cttcgggtct agcctttttc agcaactcac ttactcatca cattcaccag
    18481 aagaacgggg gtgacatacg cctcaatgac cgcagcaatg gccagcaatg gaacaaccag
    18541 cccgaggaag atcttcaacc agtcggcaaa tagctcgatg atcacctcac ccatcgattt
    18601 gcctgtttga ggcgtaacaa gagccacgcc catgtacaac atcgccgcag acccgatcat
    18661 cacagcgggc aattcaaaga tcccatgcgg cagaatacca gcggcaaaga cgggcaacgg
    18721 tgaaatgcca agcaacttca acaacgcata caacccgccg atcaagccaa tgttaaccat
    18781 gtaaagcaac acccccaaaa cgctgaacga aaccaacccc gccagaaaaa tgaccgccac
    18841 tgcgcgggta ttgttggcaa aaaggaacgg cgcagtgatg gttctatgca aatccgtcag
    18901 gcctgaaaaa tcctgtgcgc tgttcgtaat tttttgcagc ctttcagggg tggcatgttc
    18961 aatgacaggc gcaaagttga ccatcatcca atcgtagcca agccatatgc tgacaatggc
    19021 gatcaaggtc accaccagca cagcggaaga aacacgccgc aacgagccgc ccactgacat
    19081 tccataccat tcacgaagcg aatgtgcccc gccaaaaaaa ttcttccaaa atgtgcccca
    19141 aatccagcgc aggttgatcg tatcaatttc acggccaagc aggtattccc gctgaaaatg
    19201 cgcaataccc acgcggatca atagcagcga aatgatcatc acacctgcaa cggccagcca
    19261 gagcacgtct tcatttcccc aaaacaacat caccgcctca ccctgcatca gaatcgcaac
    19321 agggatcaca ataaacgagg ccagcaaatt cgcagccttc accgaggtgg attgcaccga
    19381 aatcacgatc gccgcactca ccatcaaagt ggcatgtgcg gtcgtcaaaa acaacaattg
    19441 aaccatcacc gaaagcgaag ggattccgag tccctgacgg acaaccatta ccaggtacag
    19501 ggcaatcgaa aggtaactcg ccgtcagagg tgtggctaca cccaccagca attttccaaa
    19561 atacaactgc caatcatcaa gcggcgcact caacacaggc tcaatggtgc cgcgttcctt
    19621 ttcacccaca aaggcttcca atgccaccac caacgaaatg gtgatcggaa agaaaccaat
    19681 gatcatgatc gaaaatggca caagcctgtc gaggatcaaa ttcgcttcgt attgattcag
    19741 aaaatcaacc gtctgttttg cgaattcatt catcaggaaa gggaatgcga tcacaagaat
    19801 gatcatcggc gtcagcacac gccaatcgcg gaattgatct ttcaactcac gccctgccac
    19861 cagccagacc ggtctcagtt tattgaacat ggtcacttcc tttggcatct
//
LOCUS       DAOQJC010000070        19425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1283_length_19425_cov_9.897470, whole genome shotgun sequence.
ACCESSION   DAOQJC010000070 DAOQJC010000000
VERSION     DAOQJC010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 19425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19425
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1283_length_19425_cov_9.897470"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLE39_11620"
     CDS             complement(<1..272)
                     /locus_tag="PLE39_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNE69022.1"
                     /translation="MAKTLFDKIWDSHVVTEQEGAPAVLYIDLHLVHEVTSPQAFTGL
                     RQRGLKVRRPDKTLATMDHSIPTTPIDVPIADAMAAAQIKQMETNAA"
     gene            complement(393..2027)
                     /locus_tag="PLE39_11625"
     CDS             complement(393..2027)
                     /locus_tag="PLE39_11625"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNE69023.1"
                     /translation="MTNYVKIFDTTLRDGEQSPGATMTSAEKLEVAHNLARLGVDIIE
                     AGFPAASPDDLEAVRRIALEVGNPAHPESDAKIPVIAGLARANKTDIDKAWEAVQGAK
                     KPRIHTFLATSAIHMKHKLKMDPEEVVQRVSEMVAYAKSLCADVEFSPEDAGRSDPEF
                     LYVVLGEAIKSGATTLNIPDTVGYTTPDEFFKLIDGIIKNTPGMHEGITVSVHCHDDL
                     GLATANTLAGIRAGARQAEVTINGIGERAGNTSLEEVVMALKTRHPIFGLETGIETPQ
                     LSRISKLVSNYTGMIVQPNKAIVGANAFAHEAGIHQDGMLKHQTTYEIMRPEDVGVNQ
                     TKLVLGKHSGRHALRNRLAEMGHSLDEVELDKAFARFKELADRKKVITDLDLEALIAD
                     EFYKPRDVYLLNGLQVTSGTMGMPTATIRLRGPDGIVHTIAAIGTGPVDATYKAIDAI
                     VNVPCTLLEFNIHAITEGIDALGEVTVRIQSDSSNTTMDPQSEVEYIRVYGGHGADSD
                     IIVASAKAYINALNKLIVAQTEGKGKVPNDFGVMLG"
     gene            complement(2105..3160)
                     /gene="ilvC"
                     /locus_tag="PLE39_11630"
     CDS             complement(2105..3160)
                     /gene="ilvC"
                     /locus_tag="PLE39_11630"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNE69024.1"
                     /translation="MAKINFGGVEETVVTRDEFPLEKAREVLKNEVVAVIGYGVQGPA
                     QAMNMRDNGINVIVGQREGTPNWDKAVADGWVPGKTLFSVEEAAEKGTVIQYLLSDAG
                     QRSLWPKIVECLNEGDMLYFSHGFSVTFKDDTGVVPPPNVDVALVAPKGSGTSVRRNF
                     LAGSGINASYAVHQNFTGKAEERTIALGIAIGAGYLFQTDFRNEVYSDLTGERGILIG
                     ALSGVMEAQYNVLRKHGHSPSEAFNETVEELTQSLIRLVGENGMDFMYANCSTTAQRG
                     ALDWHPRFRDAVAPVFDALYESVISGNEARITLEANSRADYREQLEKELAAFRDSEMW
                     RAGAAVRSLRPENWKKS"
     gene            complement(3260..3820)
                     /gene="ilvN"
                     /locus_tag="PLE39_11635"
     CDS             complement(3260..3820)
                     /gene="ilvN"
                     /locus_tag="PLE39_11635"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNE69025.1"
                     /translation="MNYTFIALVENRPGVLNRVASLFRRRNFNIESLAVGRTEDPEVS
                     RMTVVVDCPNGDIDAHKIEANLYKLVNVIDVQDVTRQPSVTRDLALIKVKVGPEKRAE
                     VNGLADIFRARIVDVASDSVIVEITGTEDKIEGMIELLRPLGIVEMVRTGQVAMTRGV
                     NDGVRRVPGVTGHRFEDVSEMMEMTP"
     gene            complement(3874..5559)
                     /gene="ilvB"
                     /locus_tag="PLE39_11640"
     CDS             complement(3874..5559)
                     /gene="ilvB"
                     /locus_tag="PLE39_11640"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNE69026.1"
                     /translation="MKKTGAEIMWECLVREGVEVVFGYPGGANMPIYDAMLSYPVHHV
                     LVRHEQGGAHMADGYARASGKVGVAMATSGPGATNLVTGIATAMMDSVPVVFITGQVA
                     AHLIGGDAFQETDVTGITLPITKHNYIVTRADEIAEVVREAFYIARSGRPGPVLIDIC
                     KNAQIETCEFEYPEEVKLPGYQPVDHAPKASLSEAVKLIEKARKPIILCGHGVLMSKA
                     ENELMQFAVKTQTPVASTLLGLGAFPASHELSLGMMGMHGEVHTNLAIQNSDLILAFG
                     MRFDDRVTGTLKTYAPRAKKIHIEIDPSEVHKNVAVDVPLVGDLKTVLSDLIPLVDEY
                     DHEDWKQEINGWKSEADKRSIMNWDADGKLYVAHLISDIWKATGGGAIVTTDVGQHQM
                     WTAQYYQLDKPNRWISSGGAGTMGFGLPSAIGAWFAAKDQEIWAIAGDGGFQMTAAEL
                     TTAVQEGANVKVAIMNNNFLGMVRQWQEFFFEKRYSAVNMLTPDFVKLADAHGVPARR
                     VTKREEVADAIEWARKTKGPVVLEFKVEREDAVYPMVPAGNALDQMLQRPIKQ"
     gene            complement(5618..7297)
                     /gene="ilvD"
                     /locus_tag="PLE39_11645"
     CDS             complement(5618..7297)
                     /gene="ilvD"
                     /locus_tag="PLE39_11645"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNE69027.1"
                     /translation="MRSDTVKKGYEKAPHRALLRATGLKDEDFNKPFVAIVNSYVDVV
                     PGHVHLQEFGKMVKDAVRAAGGVPFEFNTIGVDDGIAMGHMGMKYSLPSRELIADCVE
                     TMVEAHRFDAMVCIPNCDKIVPGMLLAAMRVNVPTIFVSGGAMAAGKTPDGETLDLIS
                     VFEGVGAFSAGKIDEKRLSILEKFACPSCGSCSGMFTANSMNCLMEVLGLALPYNGSA
                     LAKTPEREALARQAAAQVMTLIERDIKPRDIVTAEAIDDAFALDMAMGGSSNTVLHTL
                     ALANEAGVDYPLTRINAVADKVPHICKVSPAGKWHMEDVHRAGGIPAILNEVQRGTGM
                     LHLDRMTVTGKTLGESIQGCEIKDEEVIRRYENAHSKRGGLSILFGNLAPNGAVVKVG
                     GVSEAMMKFEGPAVIFESQDEAMAGILAGKVKAGDCVVVRYEGPKGGPGMQEMLSPTS
                     AIMGQGLGDKVALITDGRFSGGTRGACIGHVSPEAAAGGPIGALKAGDIVQIDLVARR
                     LDVKLSEAEIQSRLAALPPFQSKITSKWLKRYSYFVTSADTGAVLSTDGGR"
     gene            7804..9012
                     /gene="coaBC"
                     /locus_tag="PLE39_11650"
     CDS             7804..9012
                     /gene="coaBC"
                     /locus_tag="PLE39_11650"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNE69028.1"
                     /translation="MSILSDKHILLGVTGSIAGYKAADLASKLAQSGAKVDVLLTEAG
                     EKFVTPLTFQSVTGRRAYTDKDLWGHEAHVLHVSFGKTADLLVIAPCTADTIAKLANG
                     IADNLLTVTALAATCPLVIAPAMDGGMFNHPATQENLAKLKERGAIIIGPAEGHLASG
                     LVGTGRMVEPVEILGQIRMVLGRRYGKLAGKKVVVTAGGTQEALDPVRVITNHSSGKQ
                     GYAIAQAALDSGADVCLVTAPTALTAPVGARVVKVNSAEEMLQAVLAESADALVMAAA
                     AADFRPKHIAKDKMKKRDGIPQIELEAAPDILKTVSSTPVEKKRFRVMVGFAAESQNL
                     LENADEKLKSKKLDFIVANDISANDAGFAVDTNRVTFLFANGARENLPLTSKMEVAEI
                     IIEHISRLLE"
     gene            9015..9746
                     /locus_tag="PLE39_11655"
     CDS             9015..9746
                     /locus_tag="PLE39_11655"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNE69029.1"
                     /translation="MRVLVISDIHANYTALESVLKDAGQVDETWCLGDLVGYGPDPNA
                     VVEEIRDIPNLTCLLGNHDVAAIGKMPLETFNGDARRSLMYHEKVLSASNLDYMRSLS
                     SHTRVCGDATLAHGSPRDPLWEYILNSFSAQLNFDHFNTTSCFVGHSHIQCSFVMDEN
                     SRRVVLGQTKPDTTIQLRPKIILNPGSVGQPRDRDPRAAYAIYDTEAQTWTPRRVSYN
                     IPEVQERIRSAGLPEKHALRIADGW"
     gene            9850..10794
                     /locus_tag="PLE39_11660"
     CDS             9850..10794
                     /locus_tag="PLE39_11660"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNE69030.1"
                     /translation="MKKILIFSIIAAAMLITACGGGAAPEAPVMDVMPDSAGAPAAEQ
                     EFAPNAAFEESVANSGTGAAAAAVERIVIKNADIAIVVSDVTGRMEAIQTMAEEMGGF
                     VVASNLYQSYTNDNIEVPEATITVRVPAEKLNEALDLIKKDVVEVQSETVSGQDVTAE
                     YVDLQSNLRNLELAEKDLTRFLEEATTTEDVVNIFNQLTYYRQQIEVTKGQIKYYEEA
                     AALSSVSVRIVAEETIKPLTIGNWEPKGVALESIQDLVDFLQGFSEFLIRFLLYILPV
                     FLTIAAFIYLLFLGARAMFRKMRGDKPKKEQPQEVEKK"
     gene            10890..11600
                     /gene="rsmG"
                     /locus_tag="PLE39_11665"
     CDS             10890..11600
                     /gene="rsmG"
                     /locus_tag="PLE39_11665"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNE69031.1"
                     /translation="MENLAREALSLFNVHLTGRHILAFAAYERELLDWNQKFNLTAIR
                     DAESIRTKHFLDSLSCVQAWKATPPHRLVDVGTGAGFPGIPLKIIYPAMHLTLVESVG
                     KKVKFCQHIVDTLGLENVTVIHSRAEDIGQKAEHRESYDWAVARAVANLNVLSEYLLP
                     LVKLGGNVLAQKGESGPAEAQSAEKAMKLLGGKLKQLIPVNLPGVADDRYLVLVDKVA
                     ATPPKYPRNAGMAAKTPL"
     gene            complement(11629..12261)
                     /locus_tag="PLE39_11670"
     CDS             complement(11629..12261)
                     /locus_tag="PLE39_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE69032.1"
                     /translation="MKILLCDDQAVIRDGLEMLLTLEKDFQVIGSAQDGAEAVELAAQ
                     KQPDLILMDLKMPMMNGIEATREIRARFPQIKILVLTTYDDDEWVFDAIRAGASGYLL
                     KDTPRQKIVEAIRGTMEGKSFVDPAIAGKLLNQVASKQTQPTSVLKEKLTERELDVLR
                     LIAKGINNGDIAAHLHLSEGTVRNHVSAILEKLGVSDRTQAAVIAIQHGL"
     gene            complement(12334..13653)
                     /locus_tag="PLE39_11675"
     CDS             complement(12334..13653)
                     /locus_tag="PLE39_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNE69033.1"
                     /translation="MQAQNDSSRILRVAAWIWIGYLVAMLMMDFVLYTPFAQQAITQN
                     PPLQQDIPGQQLPPFGNQQPLPPSGRFVPIFLFYAANGAVAFLFLLFTYWKWPQDQFG
                     GVFYPLLVLMISTTPIIINTVIVPRFPQGPLSNAEGMALRQLPVLFVALALVAWEYQL
                     PHVIFFSVATTALELGLIVFNVIDTQSVYVFIFIAIIRTITFVATGIFLNLLVTRLRE
                     QQESLQQANANLTHYASTLEQLTVSRERNRLARELHDTLAHSLTAISVSLETAKAYFD
                     IEPKKAQEFLDKSLDATRTGIDETRRALKALRSSMLEDMGLGPAIRQVAESAATRFKL
                     DLDLALPNPMPSLSPDVEQTILRITQEAIENITHHSRANKFTIRLESNGHTTLTIRDN
                     GVGFDMRSKEPTGHFGLIGMRERAELAGGKLKIESEKGKGTKVVLTI"
     gene            13882..14313
                     /locus_tag="PLE39_11680"
     CDS             13882..14313
                     /locus_tag="PLE39_11680"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNE69034.1"
                     /translation="MAALSGTSTNKEIERFSRFLTVGAVGTLLDFSLLTLLKLAGLPT
                     LLANSLSFTAGLVNNFTWNRLWTFGDTVQPDWRRQLAQFTVVSLVGLALNNLIVLSLE
                     DSLGAMLGHSNWGYLPAKVIATGVVVFWNYFANRAWTFRLK"
     gene            14403..15944
                     /locus_tag="PLE39_11685"
     CDS             14403..15944
                     /locus_tag="PLE39_11685"
                     /inference="COORDINATES: protein motif:HMM:NF023218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNE69035.1"
                     /translation="MKMRSLILVMSTMALLLASCGGQPSPAAPQQGDIPQQQPPTPSN
                     EQNQPPQNQNPQTSATLSESQEAAQVAQWVKDLPVVGVDENHFSVIGTISMHMLHNNV
                     RDTIMQQGTAAFYADPALVAQAETDFQNRMNSVLPAMQDGGLLYLRDVQNFPWGLLEP
                     QQGEYHFELSDAIVNEVHAQGLEYVGVVMPFAGWDLAANGAADEICTHFFQEDYPYLA
                     YEGAMGRYADLDAFVTFLGKLVERYDGDGVDDAPGLQRGVKYWQLHNEPEGENCGQFR
                     NDPEAFVEFMQRGYEAVHAACEDCQVMNGGAAIPLWKNAPGSEFWSQYVQAGGAPYQD
                     VIAIHYNEGKHDANGGTVEDFETQIQAMYALGADKPMWVTEFGVLVGENDTQGRFTRL
                     PEKDAAAWYLRFYTAGLANGVDRFFSDNISFHDTQKNQLLLPFYVNKLLEAKLNGFTE
                     AEKIADGQYRFTVNGNPVYILWNGIPSELSGSVTATDMYGNTQTVDAATLQPSEGSPL
                     IVEIQ"
     gene            16052..18433
                     /locus_tag="PLE39_11690"
     CDS             16052..18433
                     /locus_tag="PLE39_11690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE69036.1"
                     /translation="MTTATIPTQPAFLKKNLFLGITPAFLLVIVLMAVSFGVHMVNID
                     SIGDANSYYTAAVEAMLKSWSNFFFVAAEPGGSVTVDKPPLGLWIEAVFAYFLGVSGF
                     SVSLPNILAGVLSIPLLYVMVKKYAGELAGLLAAFVMAFTPVFVATNRNNTMDGMLVF
                     FLLMAAWAFIKATEDGKLRWLLLGGFIVGLGFNIKMMQAFLPLPAFYALYFFGSKEGW
                     IRKTINLGIATALLLAVSLSWAIVVDLTPADSRPYIGSSENNTVMGLIFGHNGASRLG
                     NTGLGGNGGPQNGTPPTAPQPFDQTQGGPGQTTDQATQPQQNVTNAGGPPQEALTACE
                     GSTQGAACSFEMPFGTVNGSCIIPLNSNELACAPQQGQAAPNNQQNGQDNRQGPPQAA
                     LDACSTSSQGDACSFTLQNGNTINGSCITPPNSSELACAPQGMNPQQNGQGPDGGPGG
                     NGGTPFSQETGTPGVFRFFTSPLSKQMSWLLPFALISVVLALFAGKIRLPLESAVHKA
                     LVVWGGWLLTCVVFFSMVSGIFHSYYAIMLAPALGAMVGIGFAQLWSWGGDKKWIGAV
                     LIGASAVTLAFQLFASYQYGEQSWWMLIAVILFAVGTLSMFFVKRAAYLSLLASMLII
                     PMYWTLMTVSTHGNQSLPTAYTGSNSQQQNGPNAPRPQGDGGPNSNDSSEMLTYLQTN
                     TQDVEYLVAVPSSQQGSSWVIQTGRPVLYMGGFGGQDDVVSAEDLAEMVANGELRYVL
                     YGGDRGNKEDIANWLASSCSVVSEFSNQSNGQNQAQGPDGGGPNQASTLYMCK"
     gene            18639..19313
                     /locus_tag="PLE39_11695"
     CDS             18639..19313
                     /locus_tag="PLE39_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69037.1"
                     /translation="MKNKTLFLISILALLTLVISACASIAPATQAAEVQPAQPTVTVE
                     AVNLSQAQPSAAPLEPNMQSLQPAPDAGTQPLNAQPQGNQPMQNGQGGQPNQNNDCRP
                     DLTTAATTLGITAEELQAALGNPAQGKPDLAAAAATLGVTEDALKQAIQQAMPANCNT
                     MGGGAGNGQQPPQEAYDACIGLSEGAACTVLTPNGSLSGTCTLPPVNASTQLACAPIN
                     GPQQQP"
BASE COUNT         4644 a         5393 c         4894 g         4494 t
ORIGIN      
        1 gcggcattgg tttccatctg tttgatctgc gcggcagcca ttgcatctgc aatgggaacg
       61 tcaatcggtg tggtcgggat ggaatgatcc atcgtcgcca gcgtcttatc ggggcggcgg
      121 actttgagtc cgcgttggcg cagacctgta aaggcttgag gagatgttac ttcgtggacc
      181 agatgaaggt caatgtaaag aaccgcaggg gcaccctcct gttcagtcac gacatgagag
      241 tcccagattt tatcgaaaag agttttagcc ataaagtttc cttgtaatat gtcattgcga
      301 gaagcgcttt tgcccttcgc gacgaagcaa tctcctaata attcaaggat tgcttcgggc
      361 gaaagagcac cgccctcgca atgacgtgat acttatccca gcatcacgcc aaaatcattc
      421 ggcactttcc ctttgccttc tgtttgggcg acaataagtt tattcaacgc gttgatatat
      481 gccttggcac tggcaacgat aatatctgaa tcggcgccat gtccgccata tacacggatg
      541 tattccactt cgctttgcgg atccatggtg gtgttgctgc tatcgctttg aatacggaca
      601 gtcacctcac ccagcgcatc gatcccttcg gtaatggcgt ggatgttgaa ctccaacaaa
      661 gtacagggca cattcacgat cgcatcaatg gctttatagg tcgcatccac cgggcctgta
      721 ccgatcgcag caatagtgtg gacaatgcca tctggcccgc gtaaacgaat ggtagcggtg
      781 ggcatgccca tggtgccgct ggtaacctgc aacccgttga gcaaatacac atcacgcggt
      841 ttgtagaatt catctgcaat gagggcttcg aggtcaaggt ctgtaatgac cttcttacgg
      901 tcggccagtt ccttgaagcg cgcaaatgcc ttgtccagtt caacttcgtc aagcgagtgc
      961 cccatttcag cgaggcgatt ccgcaacgca tggcggccgg agtgcttccc caacaccagc
     1021 ttggtctgat ttacgccaac atcttcaggg cgcatgatct cataagtggt ctggtgtttg
     1081 agcattccat cctgatggat ccctgcttcg tgggcaaatg cattcgcgcc aacaatggcc
     1141 ttgttcggct ggacgatcat gcccgtgtaa ttgctgacca gtttgctgat gcgtgagagt
     1201 tgcggagtct caattcccgt ctcaaggcca aagatgggat gacgtgtctt caaggccatg
     1261 accacttctt cgagcgaggt gttccctgca cgttcaccga tgccattgat ggtcacctcc
     1321 gcctgacgtg cccccgcacg aatgccagcc agggtgttgg cggtagcaag tccaaggtca
     1381 tcatggcaat gcacagagac tgtgatgccc tcgtgcattc ccggtgtgtt cttgatgatg
     1441 ccatcgatca acttgaagaa ctcatcgggc gtggtatatc ccactgtatc gggaatgttc
     1501 aatgtagtgg cgcccgactt gatggcttca cccaaaacca catacaaaaa ctcagggtcg
     1561 gagcggcccg catcttcagg gctgaactcc acatcggcgc acagcgactt ggcgtatgcc
     1621 accatttccg aaacccgttg cacgacctct tcgggatcca ttttcaactt atgcttcata
     1681 tggatcgcag atgtggcaag aaaggtgtgg atgcgcggct tctttgcgcc ttgcacagcc
     1741 tcccaggcct tgtcgatgtc ggtcttgttg gcgcgtgcca gcccggcgat gacaggaatt
     1801 ttcgcatcag actcagggtg ggctgggttg cccacttcga gagcaattcg tctgacggct
     1861 tccagatcat cgggcgaggc ggcgggaaat cccgcttcaa taatatccac gccgagacgt
     1921 gcgagattat gagccacctc caacttctcc gcagaggtca tcgttgcccc gggagattgt
     1981 tctccgtctc ttaaagtcgt gtcgaatatt ttcacatagt tggtcatagg tattcctttg
     2041 ggtaattaga gatcagtaat tggagattat gttattccaa tctctaatta ccaattacca
     2101 aaaactaact cttcttccaa ttttcagggc gtaatgaacg taccgccgct ccagcgcgcc
     2161 acatttcaga atcacggaaa gcagcaagtt ccttttcaag ttgttcgcga taatctgcgc
     2221 ggctgttggc ctcaagagtg atgcgggctt cgttgccgct gatcacagat tcatacaatg
     2281 catcgaacac gggggccacc gcatcgcgga agcgggggtg ccagtcgagt gctccgcgtt
     2341 gggctgtggt ggaacagttg gcatacataa agtccatgcc gttctccccc accagacgga
     2401 tcagggattg ggtgagttct tccacggtct cattgaaggc ttcgctgggg gaatgtccat
     2461 gcttgcggag gacgttgtac tgcgcttcca tgactccgga gagggcgccg atcaaaatgc
     2521 cgcgttcacc ggtcaagtca ctgtacactt cattcctgaa atcggtctgg aagaggtacc
     2581 ctgcaccgat ggcgatgccc aatgcaatgg tgcgttcctc ggctttaccg gtgaaatttt
     2641 gatgcacagc atagctggca ttgataccgc tcccagcaag gaagtttcgg cggacactgg
     2701 tgcccgaacc ctttggagcc accaaagcca catccacatt gggaggaggc acaaccccgg
     2761 tatcatcctt gaaggtcacg gaaaaaccgt gcgagaaata cagcatatcg ccttcattca
     2821 aacattccac gatcttgggc cacaaagagc gctgccctgc atcagagagc agatattgaa
     2881 tgaccgttcc tttttccgcg gcttcttcca cggagaaaag ggttttgccg gggacccaac
     2941 catctgcaac agccttgtcc caattcggcg tgccttcacg ctgaccgaca ataacgttga
     3001 tgccgttgtc gcgcatgttc atcgcctgcg ccgggccctg tacaccgtac ccgatcactg
     3061 ccactacttc attcttcaag acctcacgcg ctttttcaag cggaaattcg tcgcgagtca
     3121 caaccgtctc ttccacgcca ccaaaattga tttttgccat gattactatt ctccttaaga
     3181 ttttttttag ttagttacag gtttcaagtt tcaggtttca ggctttattc atccttcatc
     3241 cttcaaaatt catcatttct tacggtgtca tttccatcat ttcagaaacg tcttcgaagc
     3301 gatgaccggt tacacccggc acgcggcgca cgccatcgtt cacgccgcgg gtcatggcca
     3361 cctgcccggt gcggaccatt tccacaatgc ccagcgggcg cagcaattcg atcatgcctt
     3421 cgattttgtc ttcggtgccg gtgatttcca cgatgaccga atccgaggcc acatccacga
     3481 tgcgggcgcg gaaaatatcg gcgaggccgt tgacctcggc gcgtttctcg gggccgacct
     3541 ttaccttgat cagagccagg tcacgggtca ccgaaggttg acgcgtgacg tcctgcacat
     3601 caatgacatt gacgagcttg taaagattcg cttcgatctt gtgcgcgtcg atatcgccat
     3661 tggggcaatc cacaaccacg gtcatgcgcg aaacctcggg gtcttccgtg cgtcccacag
     3721 ccagcgactc gatattgaaa ttgcggcgtc ggaataacga tgcaacgcga ttcaagacgc
     3781 cgggtctgtt ttcaactaat gcaatgaagg tgtagttcat atgtctctcc agtaatctcc
     3841 agtcagcaat atgtggagat cggtatttgg taattattgt tttatcggcc tttgtaacat
     3901 ctgatccagc gcattgcctg cagggaccat tggatacacg gcatcctcac gctcgacctt
     3961 gaactcgagc accacggggc ctttggtctt gcgcgcccat tcgatggcat ctgccacctc
     4021 ttcgcgtttg gtcacgcggc gggcggggac gccatgtgcg tctgcaagtt tcacgaaatc
     4081 aggcgtaagc atgtttacgg ctgagtagcg tttttcaaag aagaattcct gccattgacg
     4141 caccatgcca aggaagttgt tgttcatgat ggcgaccttc acatttgcgc cctcctgcac
     4201 agcagtggtg agctctgccg cggtcatttg gaaaccgcca tcccccgcaa tggcccagat
     4261 ctcttgatct tttgcagcaa accatgcacc aatggcagag ggcaggccaa agcccatggt
     4321 gccagcgccg cctgaagaga tccagcggtt cggcttgtcc aattggtaat attgcgcggt
     4381 ccacatttgg tgctgaccaa catccgtggt gacgatcgcg ccaccacccg tggccttcca
     4441 aatatcagag atgagatgcg ccacgtacaa ttttccatcc gcatcccaat tcatgatgct
     4501 gcgtttatcg gcttcagatt tccagccgtt aatttcctgc ttccaatctt cgtggtcgta
     4561 ttcatccacc agcggaatga gatcgctcaa aacggttttg aggtcgccca ccaggggaac
     4621 atccactgct acgttcttat gcacttccga aggatcgatc tcaatgtgga tcttcttcgc
     4681 acgcggcgca taggtcttga gcgtgcctgt cacacggtca tcgaaacgca taccaaaggc
     4741 gaggatcaaa tcggaatttt ggatcgccag gttggtatgg acttcgccat gcatccccat
     4801 catgccgagg ctcaactcat gcgaagcagg gaaggcaccc aacccaagca gggtgctggc
     4861 aaccggggtt tgcgtcttca ctgcaaattg catcaattca ttttcagcct tcgacatcaa
     4921 aacaccatgt ccgcacaaaa tgatcggctt gcgggctttc tcgatcaatt tcacagcctc
     4981 gctcaacgaa gccttgggag catggtccac gggttgatag ccgggcagtt tcacttcttc
     5041 cggatattcg aactcgcagg tttcgatctg ggcattctta caaatatcga tcaacacggg
     5101 gccggggcgc ccgctgcggg caatgtaaaa agcctcgcgc accacttccg cgatctcatc
     5161 ggcacgggtc acaatgtaat tgtgtttcgt gatcggaagt gtaatgcccg tcacatccgt
     5221 ttcctgaaat gcatcgccgc cgatcaaatg cgcggccacc tggccagtga tgaacaccac
     5281 agggaccgaa tccatcatgg cggtggcaat acccgtcaca agattcgttg caccaggacc
     5341 cgatgttgcc atggccaccc caaccttacc cgaagcgcgt gcatatccat ctgccatgtg
     5401 tgcgccgccc tgttcatgac gcaccaacac atgatgcacc ggataactaa gcatcgcatc
     5461 gtagatcggc atgttcgccc cgcccggata cccgaagacc acttccacgc cttcgcgcac
     5521 caaacactcc cacataattt ctgcacctgt ctttttcatt ctcttctcct tgatttagac
     5581 cagagaccgt tgagcacaaa ccatgatggc ctgttgccta ccgcccaccg tctgtagata
     5641 acactgcgcc tgtatctgcg ctggtgacga aatacgaata tcgctttaac cactttgaag
     5701 tgattttgga ttggaatgga ggcagcgccg ccaagcgact ctggatctcc gcttcgctca
     5761 acttaacgtc cagcctgcgt gccaccaaat caatttggac aatgtccccg gcttttagcg
     5821 ccccaattgg accacccgca gcagcttccg gcgacacgtg accaatacat gcgcctcgcg
     5881 ttccaccact gaagcggcca tcggtaatga gtgccacctt gtcgccaagt ccctgcccca
     5941 tgatcgcaga ggtgggcgaa agcatttcct gcataccggg tccgcccttt ggcccttcat
     6001 aacggaccac cacacaatcg ccagccttga ccttgcccgc caaaattcca gccatggcct
     6061 catcctgcga ttcaaaaata acggcagggc cttcaaactt catcatggct tcacttacgc
     6121 cacccacttt gaccactgcc ccattcggcg ccaggttgcc aaacaaaatg gataaaccac
     6181 cacgtttgga gtgggcattt tcatacctgc gaatcacttc ttcatctttg atctcacaac
     6241 cttgaatcga ttcccccagt gtctttcctg tgaccgtcat gcggtccaaa tgcagcatac
     6301 ccgttccccg ttgcacctcg ttcaaaatgg cagggattcc ccccgcgcga tggacatctt
     6361 ccatatgcca tttgcccgcc gggctgactt tgcagatgtg aggcaccttg tctgccacgg
     6421 cattgatgcg ggtcagcgga taatccacac ctgcttcatt agccagagcc agcgtatgca
     6481 gcaccgtgtt ggaagaccca cccatcgcca tatccaaggc aaacgcatcg tcaatggctt
     6541 cagcagtgac aatgtctcgc ggcttgatat cgcgttcgat cagggtcatg acctgcgcag
     6601 cggcctgcct tgccaatgct tcgcgttcgg gggtcttcgc cagtgcagat ccgttgtacg
     6661 gcagagccaa tccgagcact tccatcaaac agttcatgga atttgcggtg aacatgcccg
     6721 agcatgaacc gcaggatggg caggcaaatt tttccaaaat ggataaacgc ttttcgtcaa
     6781 ttttccccgc tgaaaacgca cccacccctt caaaaactga gatcaggtca agggtttcgc
     6841 catcgggtgt cttgcctgca gccatcgcac caccagagac aaaaatggtc ggcacgttca
     6901 cgcgcatggc ggccaacagc atgcctggca cgatcttgtc acagtttgga atacagacca
     6961 ttgcatcgaa tcggtgggct tcgaccatcg tctccacaca atctgcgatc agttcgcggg
     7021 aaggaagtga atacttcatc cccatatgcc ccatggcaat tccatcgtcc acgccaatgg
     7081 tattgaattc aaaaggcaca ccgcccgccg cacggacagc atccttcacc atttttccga
     7141 attcctgcaa atgcacgtgc ccggggacca catctacata agaattaacg atggcaacaa
     7201 aaggcttatt aaagtcttcg tctttcaaac ccgtggcacg caacaatgca cggtgtggtg
     7261 ctttctcata ccctttttta actgtatctg atcgcataat gctcctttga tgtgtagtgc
     7321 ttgaccgtgg actgcggtca atcttctgta gcctgttttt tatacaaaaa gagccgcccg
     7381 tggttagggc ggctcttcgt acttacttag tgatttactt tactctcacc gtagccatcc
     7441 taaccgaaaa cagatcctta ataataagga ccgcaaggac tactacaata agggagaggg
     7501 tgaacaaaga tgtgctcatg ggttgtgact tcgcccgatt ataggcagat tcggaaataa
     7561 gtcaaggggg gttgtgtcgg attcggggat gctaatcgtt tacattttgt agaatcatca
     7621 acgttcctga atagggcatc tattcacctt cgctaggaaa acgccctgct gaaacaccta
     7681 aatttggtat ttggtctgta gagttggtcc aatcatttgc gctgagttgg caagtcttgc
     7741 aacacaacaa cataccagca aaaaagcact tacattttcc ccttggaagt ataatgacca
     7801 tatatgtcca tcctttccga taaacacatc ctgcttgggg tgacgggttc cattgcgggc
     7861 tataaagccg cagatctggc atccaagctt gcgcaatcag gcgcaaaggt ggatgtgctt
     7921 ctgaccgaag ccggggaaaa gttcgtcaca ccactcacct tccaatctgt cacaggccgc
     7981 cgcgcatata cagataaaga cctatggggc cacgaagccc atgtcttgca tgtcagcttt
     8041 ggaaagacag ccgacctgct ggtcattgcc ccttgcacgg cagatacaat tgcaaaactc
     8101 gcgaacggca ttgccgataa tctcttaacg gtgaccgccc ttgcggcaac ctgtccattg
     8161 gtgatcgcac ctgccatgga tggcggtatg ttcaaccacc cagccacgca ggaaaatctt
     8221 gcaaaattaa aggaacgtgg cgcaatcatc attgggcctg cagaaggtca ccttgcttcg
     8281 gggttggttg gcacaggccg aatggttgag cctgttgaaa ttctcgggca aattcggatg
     8341 gtgctgggca ggcggtatgg aaagctcgcc gggaaaaagg ttgttgtcac ggcaggcgga
     8401 actcaggaag cgcttgaccc ggtgcgcgtc atcaccaatc attcatcggg gaaacaggga
     8461 tatgccatcg cccaggccgc gctcgattca ggcgcagacg tttgtctggt cactgctccg
     8521 acggcattga ccgcccctgt gggagcgcgt gttgtcaaag taaacagtgc ggaagaaatg
     8581 ctccaggctg tattggccga gtcagcagat gcgctggtga tggccgcagc cgcggcggat
     8641 ttccgcccga aacacattgc aaaagacaaa atgaaaaagc gcgacggtat tccgcagatc
     8701 gagcttgaag cggcgcccga tatccttaaa acagtatcaa gcacacccgt tgaaaagaag
     8761 cggtttcggg tcatggtcgg ttttgcggcg gagagtcaaa accttttgga gaacgcagat
     8821 gaaaagctga aatccaaaaa actggatttc atcgttgcga acgacatttc tgcaaacgac
     8881 gcagggtttg ccgtggatac gaaccgcgtc acatttttgt ttgcaaatgg agccagggaa
     8941 aatttgccgc tgacaagcaa gatggaagtg gctgaaatca tcatcgaaca catatcacgg
     9001 ttgctggagt gaaaatgcga gttttggtca tttctgatat tcatgcaaat tacaccgccc
     9061 ttgaatctgt cttaaaagat gcgggccagg tggatgaaac ctggtgtctt ggcgaccttg
     9121 ttggctacgg cccagacccc aacgcggtgg tggaagagat tcgcgacatc cccaacctga
     9181 cctgcctgtt gggcaaccat gacgtggccg ccattggcaa aatgccgctt gaaactttca
     9241 acggggatgc gcgccgatcg ttgatgtatc acgaaaaggt gctttcagcc tccaacctgg
     9301 attatatgcg gtcgctctct tcgcacacgc gggtatgtgg agatgccacg ctcgcacacg
     9361 gcagcccgcg cgacccgttg tgggaataca ttttgaattc gttttcagcg cagcttaatt
     9421 tcgaccattt caacacaaca agttgtttcg tggggcattc ccacatccaa tgctcgtttg
     9481 tcatggatga aaattccagg cgggtcgttc tcggccagac caaacccgat acgacaattc
     9541 agttacgccc gaagatcatc ctcaacccgg gttcggtggg ccagccgcgt gaccgcgacc
     9601 cgcgtgccgc gtacgctatt tacgacaccg aagcccaaac atggactccc cgccgcgtga
     9661 gttacaacat tcccgaagtg caggaacgca tccgttcggc tggtttgcct gaaaagcacg
     9721 ccctgcgcat tgctgatggc tggtagtact aaagttgggc cagagaaaat cgggtgtcag
     9781 gaactttctc tcgatcatgt tcgttcctta ggtagagaaa tcaccataag gtaaaaagga
     9841 gaaaagatca tgaaaaagat tttgatattc agcattatcg ccgccgccat gctgatcacc
     9901 gcctgtggag gtggtgcagc ccctgaagcc cccgtcatgg atgtgatgcc tgactcagcg
     9961 ggtgcgcccg cagcagagca ggaatttgcg cccaatgccg catttgaaga aagtgttgcc
    10021 aatagcggca caggtgctgc cgccgccgca gttgagcgga tcgtcatcaa gaacgccgac
    10081 attgccattg tggtttccga tgtaacaggc cgcatggaag ccattcagac catggctgag
    10141 gaaatgggcg gatttgttgt tgcctcgaac ctgtaccaaa gttacaccaa tgacaatatc
    10201 gaagtacccg aagcgacgat caccgtccgt gtgcccgctg aaaagttgaa tgaagcgctc
    10261 gatctcatca agaaggatgt ggttgaggtg cagagcgaaa cggtctccgg gcaggatgta
    10321 accgccgaat acgtggattt acaatcgaac cttcgaaatc ttgagcttgc tgaaaaggat
    10381 ctgaccagat tcctcgaaga agccaccacc accgaagatg tcgtcaatat tttcaaccaa
    10441 ctcacctact accgccagca gatcgaagtg accaagggac agatcaaata ctacgaggaa
    10501 gccgccgcac tttcttcggt cagtgttcgc atcgtcgctg aggaaaccat caaaccgctt
    10561 accatcggca attgggaacc caagggtgtg gcgctcgaat caatacagga tctggttgac
    10621 ttcctccagg gcttttctga attcctgatc cgtttcctgc tctatatcct gcctgtcttc
    10681 cttaccattg ccgctttcat ttacctcttg ttcctcggcg cacgggccat gttccgcaag
    10741 atgcgtggag ataaaccaaa gaaagaacaa ccgcaggaag tggaaaagaa gtaagaagtg
    10801 ttcagtcagt aggaaattgg ggatagtgaa tagggaatag taaagagagg ccgctgaaaa
    10861 gtggcctctc tttttgctat aatcacctca tggaaaatct ggcccgcgaa gcactctcgc
    10921 tcttcaatgt tcacctgacg ggaaggcata ttctggcatt tgccgcctac gaacgtgagc
    10981 ttttggattg gaatcaaaaa ttcaacctga ccgccattcg agatgcggaa tccatccgta
    11041 ccaagcattt tttggattca ctatcctgtg tgcaggcgtg gaaggccact cccccgcatc
    11101 gcctcgtgga cgtgggaacg ggtgccggtt tcccgggcat tcccctgaaa atcatctatc
    11161 ctgccatgca tttgactttg gttgaatcgg tcggaaagaa ggtgaaattc tgccaacaca
    11221 ttgtggatac gcttgggttg gaaaatgtga ccgtgatcca ttcacgggcg gaagatatcg
    11281 gccaaaaagc ggaacaccgc gaaagctatg actgggctgt ggcccgtgct gtggccaacc
    11341 tcaacgtttt gagcgaatac ctgcttccac tggttaagct gggcggaaac gtgctcgccc
    11401 aaaaaggcga aagcggaccc gccgaagcgc agtccgctga aaaggcgatg aaattattgg
    11461 gtggaaaact gaaacaactg atccctgtga acctgccagg ggtggccgat gaccgctatc
    11521 tcgtgcttgt agataaggtt gccgccacgc cgccaaaata tccgcgcaat gcagggatgg
    11581 cagcaaaaac tccgttgtga gtcaacaact gttcgccgaa aaccgtcctc acaacccatg
    11641 ctgaatggca atgactgccg cctgggttcg atcggaaact cccaacttct ccaaaatcgc
    11701 gctgacgtga ttccgcaccg tgccttcaga taaatgcagg tgtgcggcga tatcaccgtt
    11761 gttaattccc ttggcgatca aacgcaaaac atccaattca cgctcggtga gcttttcttt
    11821 caaaacagag gtgggctggg tttgtttgct ggcaacttga tttaataact ttcccgcaat
    11881 ggcagggtca acaaaagatt tgccttccat cgtgccgcga atggcctcca cgatcttctg
    11941 gcgcggtgta tctttgagca ggtaccctga agcgcctgcg cggatggcat cgaagaccca
    12001 ttcatcgtca tcgtaggtcg tcagcacgag gatcttgatc tggggaaaac gcgcgcgaat
    12061 ttcccgcgtg gcttcaatgc cgttcatcat cggcattttc aggtccatca aaatcaggtc
    12121 gggttgtttt tgcgcggcaa gttccacggc ttctgcgccg tcttgcgcag agccgatgac
    12181 ctggaaatcc ttttcaaggg tgagaagcat ttccaatcca tcgcggatca cagcctggtc
    12241 gtcacagaga agtattttca tggttgaatt ctcagcaatg gacgatggat cacagaccgt
    12301 tttacatcag ccatcgttgc ctgcctgcaa ggattatatc gtcagcacaa ccttcgtgcc
    12361 ttttcccttt tcgctttcga tctttaattt accgccagca agttcggcgc gttcacgcat
    12421 accgatgagg ccgaaatgtc ccgtcggttc ttttgagcgc atgtcaaaac caacgccgtt
    12481 atcacggatg gtcaatgtgg tatgcccatt gctttcaagg cggatggtga atttgttggc
    12541 gcgtgaatga tgggtgatgt tttcaatggc ttcctgcgta atgcgcagga tggtttgctc
    12601 cacgtctggg ctgagagaag gcatggggtt cggcagagcc agatcaaggt ccagtttgaa
    12661 gcgagtggcc gcagattcgg ccacctgtcg gatggcgggt cccaatccca tatcttccag
    12721 catggatgaa cgcaaggctt tcaaagcgcg gcgggtttca tcaatgccgg tgcgggtggc
    12781 atccaatgac ttatcgagaa attcctgcgc ttttttaggt tcgatatcga aataggcttt
    12841 ggcggtttca agcgaaacgg agatcgcagt gagggaatga gccagcgtgt cgtgcaattc
    12901 acgggcaaga cgattgcgtt cccgactcac cgtcaactgc tccagcgttg acgcataatg
    12961 cgtgagattg gcattggcct gttgcaaaga ttcctgctgc tcgcgcagac gggtgaccag
    13021 caggttaagg aagatgcccg tcgctacaaa tgtgatggtg cgaatgatgg cgatgaaaat
    13081 aaacacataa acgctttgcg tatcaatgac attgaaaacg atcaacccaa gttcaagcgc
    13141 cgtggtggcc acgctgaaaa aaatgacatg cggaagttga tactcccatg ccaccaatgc
    13201 cagagccacg aacagaacag gcagttgacg cagcgccata ccctccgcat ttgaaagcgg
    13261 accttgcggg aagcgcggca cgattacagt attgatgatg atgggtgtgg ttgaaatcat
    13321 caacaccaac agcggataaa acaccccgcc aaactggtct tgcggccatt tccaatacgt
    13381 gaataacagg aagagaaaag caaccgcgcc gttggcggca taaaaaagga atatcggcac
    13441 aaaacgcccc gacggcggca ggggttgctg gtttccaaag ggcggcaact gttgaccggg
    13501 gatatcctgt tggagtggcg ggttctgtgt aatggcctgc tgggcaaatg gagtgtacaa
    13561 aacaaaatcc atcatcaaca tggcaaccag gtagccgatc cagatccatg cggcgacacg
    13621 cagaattcga gaggagtcgt tttgggcttg catggacaaa tcttatcatg aaggcgggtg
    13681 gatgccctgt gcgcgtcgtc atgattcccc cacatttccc atgacagctg tcatgggtgg
    13741 ttgtgaggaa ttcacgcctg caaacatcaa gtaatgactg gcttcactct ctcattttgg
    13801 ccgaaaaata taagattggg atatcgaaag caacaacgtc tttgttcatc tctgcttatt
    13861 tctggttcaa aggagcaaaa aatggccgct ttatctggca catccacaaa caaggaaatc
    13921 gaacgcttct cacggtttct gaccgtgggc gcggtcggca ctttattgga tttcagcctg
    13981 ctgaccctgc tgaaactggc aggcctgcct actttgctgg caaacagttt gtctttcaca
    14041 gcaggcttgg tcaacaactt cacctggaac cgcctgtgga ccttcggcga cacagttcaa
    14101 cccgattggc gccgtcaact ggctcaattc acggttgtaa gtttggtggg gcttgcatta
    14161 aacaacctga tcgtactttc gcttgaagac agcctcggcg caatgctcgg ccattccaac
    14221 tggggttacc tccccgccaa ggtcattgcc acaggtgtgg ttgtgttttg gaattatttt
    14281 gccaaccgcg catggacctt ccgtttgaaa taggtttatg gaaacaccca ccgcacacga
    14341 gacgatttga gtctcttttt accgacaaaa cctgcggtgt atttaaacaa ggagaagaaa
    14401 acatgaaaat gaggtcatta attttggtaa tgtctacaat ggcactgttg ctggcttcct
    14461 gcggcggcca accatcccct gccgccccgc aacagggaga tattccacag caacagccac
    14521 ccaccccatc gaacgaacag aaccagccgc ctcaaaacca aaatcctcaa acttcagcca
    14581 cgctgtcaga atctcaagaa gcggcccagg tggcccaatg ggtaaaagac ctgcccgtgg
    14641 ttggtgtgga tgaaaatcat ttttcagtga tcggaaccat ttccatgcac atgcttcata
    14701 acaacgtgcg ggacacgatc atgcaacagg gcacggctgc cttttatgcc gatcctgcac
    14761 ttgtggccca agctgaaacg gattttcaga atcgcatgaa cagcgtcctg cctgccatgc
    14821 aggatggcgg tttgttgtac ctgcgtgatg tccaaaattt tccctgggga ttacttgaac
    14881 cgcaacaggg cgaatatcat tttgaattaa gcgatgccat tgtcaatgaa gttcatgcgc
    14941 aaggactgga atacgtggga gtggtcatgc ccttcgcagg ctgggacctt gccgcgaacg
    15001 gggcggcaga tgaaatctgc acacatttct ttcaggaaga ttatccctac cttgcttatg
    15061 aaggcgccat gggccgctat gccgacctgg atgcctttgt taccttcctt ggaaaattgg
    15121 ttgaacgcta cgacggcgat ggcgtggatg atgcgcccgg actgcagcgt ggtgtgaaat
    15181 actggcagct tcataacgaa cccgaaggcg aaaactgcgg acaattccgt aatgaccccg
    15241 aagccttcgt ggaattcatg cagcgcggat atgaagctgt ccatgctgcc tgcgaagatt
    15301 gtcaggtcat gaacggcggc gcagccattc cactttggaa gaacgccccc gggtccgaat
    15361 tttggagtca gtatgttcag gcaggcggtg cgccctatca ggatgtcatc gccatccact
    15421 acaacgaagg caaacatgat gccaatggtg gaaccgtgga agatttcgaa acgcagatcc
    15481 aggccatgta tgcccttggt gcagataaac ccatgtgggt cacagaattc ggcgttctag
    15541 tcggtgaaaa tgatacacag ggacgtttca cccgcctccc cgaaaaagat gccgccgcct
    15601 ggtacctgcg tttttatact gccgggctgg ccaacggcgt ggaccgattc ttctcggaca
    15661 atatttcctt ccacgacaca caaaagaacc agctcctgct gccgttctat gtaaacaaat
    15721 tgctggaagc caaactcaac ggcttcaccg aagctgaaaa gatcgccgac ggacaatacc
    15781 gcttcaccgt gaacggcaat cctgtatata tcctctggaa cggaatccca tccgaactaa
    15841 gcggctccgt aactgcaaca gatatgtacg gcaacacgca aactgtcgac gctgccactt
    15901 tgcaacccag cgaaggatcg ccgctgatcg tggaaattca ataaaacaaa cgaagatcaa
    15961 aggttttgtc ggtaaaaaaa gattcaaaag ctcaaacacc ccgcgcggcg aaaaggactt
    16021 aaacaatacc gcgcaaaaga aggaacaatc aatgacaact gcaaccatcc ccacccaacc
    16081 tgccttcctg aagaagaacc tcttcctcgg gatcaccccc gcctttctgc ttgtgatcgt
    16141 cctcatggcg gtttctttcg gcgtacacat ggtcaacatc gactccattg gcgatgccaa
    16201 ctcatattac accgccgctg tggaagccat gctgaaaagc tggagcaatt ttttcttcgt
    16261 cgctgccgaa cccggtggct cggtcaccgt ggataaaccc ccgctggggc tgtggatcga
    16321 agcggtcttc gcatacttcc tcggcgtaag cggattcagc gtttcgctgc cgaacatcct
    16381 cgcgggcgtg ctcagcattc cacttttgta tgtcatggtc aaaaaatacg caggcgaact
    16441 tgcaggcttg cttgccgctt ttgtcatggc gttcacaccc gtcttcgtgg ccaccaaccg
    16501 caacaacacc atggatggga tgctcgtctt ctttttgctc atggcagcct gggctttcat
    16561 caaagcaacc gaagatggca aactccgctg gctcttactc ggcggattca tcgtcggtct
    16621 cggcttcaac atcaaaatga tgcaagcctt cctgccgctt cccgctttct acgcactgta
    16681 tttcttcggt tccaaagaag gctggatccg caaaaccatc aacctcggca ttgccaccgc
    16741 attattgctc gcggtgtcac tctcatgggc gatcgttgta gatttgaccc ccgcagactc
    16801 tcgtccctac atcggctcca gcgaaaacaa cacagtcatg ggattgatct tcggccacaa
    16861 cggagcctca cgcctcggaa acaccggtct gggaggaaat ggcggcccgc aaaatggaac
    16921 gcctcccacc gcgccgcaac ccttcgatca aactcagggc gggcctggtc aaacaaccga
    16981 tcaggcgact cagccccagc agaatgtgac caatgcaggc ggcccaccac aggaagcgct
    17041 cacagcctgt gaaggatcaa cgcagggagc cgcctgttct ttcgagatgc cgttcggaac
    17101 cgtgaatggc tcctgcatca tcccgctcaa ttccaatgaa ctcgcctgcg ccccgcaaca
    17161 aggacaggcc gcccccaaca atcaacaaaa cggacaggac aatcgtcagg gcccgccaca
    17221 agccgcattg gatgcctgtt caacatcttc acaaggcgat gcctgctcat tcacactgca
    17281 aaacggaaat acgatcaacg gctcttgtat tacgcccccc aattccagcg aactcgcctg
    17341 cgcgccgcaa ggtatgaatc ctcaacaaaa cgggcaggga cctgatggcg gcccgggcgg
    17401 caatggcggc actcccttta gtcaggaaac gggcacaccg ggcgtcttcc gcttctttac
    17461 cagcccgctc tccaaacaaa tgtcgtggct gctgcctttc gcgttgatca gtgtggtgct
    17521 ggcgcttttc gcgggcaaga tccgcctgcc gcttgaatcg gcggttcaca aagcccttgt
    17581 cgtctggggc ggatggttgt tgacctgtgt ggtgttcttc agcatggtct ctggcatctt
    17641 ccactcttac tatgccatca tgctggctcc cgctctcggc gccatggtag ggattggctt
    17701 tgcccaactg tggagttggg gcggcgataa aaaatggatc ggtgcggtac tcatcggtgc
    17761 atcggcggtg actctggcct tccaactctt tgcttcatac cagtatggcg aacaatcctg
    17821 gtggatgctc attgcggtga tcctgttcgc cgtgggaacg ctttcgatgt tttttgtgaa
    17881 gcgcgcggcc tatctcagcc tgctggcttc gatgttgatc atccccatgt actggacgct
    17941 catgaccgta tccacgcatg gcaaccaaag cctgcccaca gcctacacag gcagcaacag
    18001 ccaacagcag aacggaccca acgcaccccg cccccaagga gatggtggac cgaactcgaa
    18061 cgacagcagc gaaatgctca cctacctgca aaccaacaca caggatgtgg aatatctggt
    18121 ggcagtgcct tcttctcaac aaggttcctc ctgggtgatt caaacaggga gacctgtgtt
    18181 gtacatgggc ggcttcggcg gacaggatga tgtggtcagt gcggaagacc tggcagaaat
    18241 ggtagccaac ggcgaactgc gctacgtcct ttacggcggc gaccgcggca acaaggaaga
    18301 catcgcaaac tggctggcca gttcatgttc ggtggtctct gaattcagca accaatccaa
    18361 tggtcagaat caggcacaag gtccagatgg aggcgggccg aatcaggctt cgaccttgta
    18421 catgtgcaag taatcccaat ttttagacac aaaaaacgct gacgaagcaa cgtcagcgtt
    18481 ttttgttttc acaggtactc cattcttccc atgacacctg tcatgcccgt tgatgcaagc
    18541 ctgcatggac gttcgctcaa catgatgatt ttacacactc attacatttt ttacgcgtgg
    18601 ctaaaatgca gccacatcaa atttgaaagg aaaacaaaat gaaaaacaaa actctcttcc
    18661 tgatctccat cctcgccctg ctgactctcg ttatcagtgc gtgtgcaagc attgcccccg
    18721 ccactcaggc cgctgaagtt cagcctgccc agccaactgt gaccgtggaa gcggtgaacc
    18781 tttcgcaggc ccagccatcc gctgccccgc ttgagccaaa tatgcaatct cttcaacctg
    18841 ctccggatgc cggcacacag ccgctgaatg cccagccaca gggaaaccaa cccatgcaaa
    18901 acgggcaggg cggacaaccc aatcaaaaca acgattgccg ccccgatctg accacagccg
    18961 caaccacact gggcattacc gctgaagaac ttcaggccgc attgggcaac cctgctcaag
    19021 gaaaacctga cctggccgcc gcagccgcca ctctcggcgt cactgaagat gcgctgaaac
    19081 aggccattca acaggccatg cccgccaatt gcaacaccat gggcggcgga gcgggcaacg
    19141 gtcagcagcc cccgcaggaa gcctacgatg cctgcatcgg attaagcgaa ggcgctgcct
    19201 gcaccgtcct cactccaaat ggaagcctga gcggcacctg caccctgccg cctgtcaatg
    19261 cctccaccca gttggcatgt gcccccatca acgggccgca acaacagcca taagatatta
    19321 gaactcttgc ataagtccat ttttttacca caacacttgc gccgcacgcc agtgcaggtg
    19381 aggcacaaag tctcgaagac tcaaagaaaa aaagcttggt gcctt
//
LOCUS       DAOQJC010000071        19228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1310_length_19228_cov_8.954624, whole genome shotgun sequence.
ACCESSION   DAOQJC010000071 DAOQJC010000000
VERSION     DAOQJC010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 19228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19228
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1310_length_19228_cov_8.954624"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..2687
                     /locus_tag="PLE39_11700"
     CDS             18..2687
                     /locus_tag="PLE39_11700"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HNE69038.1"
                     /translation="MNPIPKLTSLGQSLWYDNIQRKLLENGELKAMIERGDIRGVTSN
                     PTIFQNAIAKTTDYDAALIPLAWAGWDAERIFWQVAVEDIQEACDLFRPLYDETRGGD
                     GYVSLEVSPFLANDTEGTQKQAKELWERVNRPNLMVKIPATKAGIPAIRSTIAAGINV
                     NVTLIFSLDRYAAVMDAYLAGLEDRIAANESIEMVASVASFFVSRVDTKIDPRLPEGS
                     PLRGRAAIANAKLAYDAFESVFTSPRFATLKARFRARVQRPLWASTGTKNPAYSDTLY
                     VDTLIGPDTVNTVPPATLDAFRDHGKASLTITRGLEEAQLFFAELKAAGISMGSVTQE
                     LEEEGVKAFDDAFKTLLQAIEDRRKSAVSSLGPLADSVAKRLTTLEEHSVAARIWAHD
                     PTLWASDPEGQAEIKIRLGWLHSIEDARTRLDGYLSFAKQIHEEGIDRVLVIGMGGSS
                     LTAEVLSSLMAAANVDAKLSLAILDSTDSEQVALAAKNYPPEKSLYILASKSGGTAEL
                     LAAFDYFWELSKGNGSRFMVTTDAGTSLEKLAKDRGFRKVFNADPNVGGRFSALTDFG
                     LVPAALLGMDIEKLLASAEKIKNASHKNVSAGFALGALLAESALAGRDKLTVLSDAPV
                     SALAGWIEQVIAESSGKHGKGILPVPMEPLGSPDVYGDDRLFVYLRNNGELSDGIAAL
                     KNAGFPVVELPIANLYDAGAEFFRWEIAISVACHILGINTFDQPDVQDSKLRTIAKIK
                     DYQATGKLAEADLVDVADAKSALEKFMTGAQAGEFFTINAYLPRNKETENALQALRVA
                     IRAKTKCAVSAGFGPRFQHSTGQFHKGGPNKGRFIQVVYDAQNDMDIPNQGMTFGTLI
                     RAQALGDYEALVAAGRKVLRVKLNSVEDLKKLL"
     gene            2734..3348
                     /locus_tag="PLE39_11705"
     CDS             2734..3348
                     /locus_tag="PLE39_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69039.1"
                     /translation="MKYDPQVHHRRSIRLKGYDYTQADAYFITICSYQREHVFGEVVN
                     GEMKLSAWGEIARAEWFKTAALRDNVELYEDEFVVMPNHIHGIVWLHEVGTRRRRVPT
                     EKFGKPVAGSIPTIIRAYKSAVTYAINGLEKSRGGAIWQRNYYEHVIRNEKELNTIAR
                     YIYYNPFNWQLDRDNANNIRNIPLPETIDDYLLDAEKMVGIKAL"
     gene            complement(3379..4941)
                     /locus_tag="PLE39_11710"
     CDS             complement(3379..4941)
                     /locus_tag="PLE39_11710"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNE69040.1"
                     /translation="MTKIHFSKIQTESKIIAARELQIELPSTPVKYYRHGWQSWSLAA
                     WTSLSPLPVQKPAIFHPLQVDAVTVHESRPNGSWLGAVEFADGNVLLLGALATDTTVL
                     LEQDQLTGRSEAGDVEWFMAFGREDVIFAEYAKALEHRFGSIQKNDTPRVWCSWYSLY
                     HAIDEPALHKIFDELTDLPFEVLQVDDGWQMDIGDWTVNEKFPSGMTALAEKIKSTGR
                     RAGLWLAPLIATKTSQLFRTHADWFLRDEHGQLVSAGFNWGQPLYALDTTHPDVTPWL
                     VALMKQVRAWGFDYLKLDFLYGGALKGKRHNDIPREAAYRESLRVMREAMGADAFFLT
                     CGTPILPALGLCDAMRIGPDVGAHWEKHRDSVLFYNFAIPGTRNAIRTVINRLWLDPL
                     VRIDPDVVYFESNENSLTPEQKSLLQDLALICKFKATSDLPQWMTTDEYEHLRAYLNL
                     TPAIKQTGRYTFEIDNRNVDFTPATELPQIPTGLAALWGSLLSWLGDFHFVLRIFKWL
                     DDEALRKRRASL"
     gene            complement(4990..5967)
                     /locus_tag="PLE39_11715"
     CDS             complement(4990..5967)
                     /locus_tag="PLE39_11715"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNE69041.1"
                     /translation="MKNRNLLLYVISGILSAVLLAYLLLNLDWEILRVAFSDLHWGWL
                     GMAFLAYLLNIFLRALRFTNLIYSRPVKWIELVPVSALHNILMYLMPAKTGDVAYIFL
                     AKDRLDLSLTEGTATLLAARYYDFSVVGLVLAILLPFSKKDMPVWIFESSVIFCLLIL
                     LGTIGILLFLRFSKPATDNQPVGEKTLITRIRAAWTKFIAGLREIQNHGAHGRVALLT
                     AGIWLCVYSNFYFATRSMGLPVAFYHIAIISIVMIPLTLLPLQGFANIGTHEVGWTSV
                     LVAFGYPYKTALAIAAGSHLVLLFSVLISGGVAFFGAQIISKSIKHEGN"
     gene            complement(5972..6790)
                     /locus_tag="PLE39_11720"
     CDS             complement(5972..6790)
                     /locus_tag="PLE39_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNE69042.1"
                     /translation="MKDTQPILIISTGRTGTIFFSRLFADLYPDSVSYHERGASRPIQ
                     ILTNLHFSHLFPKSGLKTAWKLLKGNEIETCEAKFHIDANCFLYGLAPLAPELYPNLK
                     VLHIVRDPRSYVTSHLNFSRQKGTSFIANYFVPFWQPNPFLVGELPLSKAIGFTRFEK
                     YCWIWDFKNRVMEGLENTSTPYMRVRFEDLFNTDNPEDLFGRITDFLGLPRVTGIREK
                     FRQPANTSSKTDFPEWPDWTPKQAAQLQALCGARMAKYGYGGESEWEKKVIGNW"
     gene            complement(6787..7707)
                     /locus_tag="PLE39_11725"
     CDS             complement(6787..7707)
                     /locus_tag="PLE39_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE69043.1"
                     /translation="MPTEKKTSPKITVIIPAFNEGEVIGGVIGRIRAVVNTLDPDNEI
                     IVIDDGSKDNTAQSARDAGARVIEHPYNIGNGAAVKTGIRNARGQILVTIDGDGQHDP
                     NDIIKLVNRIGPYDMVVGSRNRESDTAAHRDVANMVFNSLATYVSGRKIEDLTSGFRA
                     IKAHIARQFVYLLPNQFSYPTTITLSVVRAGYSLGYESIRFARREGKTKSKIKPLRDG
                     LRFLMIILKIAVFYAPLKIFVPASIFTFVLGVAYGLMRIFILNAPYGQTSALLMSTAA
                     LIFMVGLVSEQIAQLRFDRSESNTPLHKDE"
     gene            7879..9837
                     /locus_tag="PLE39_11730"
     CDS             7879..9837
                     /locus_tag="PLE39_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69044.1"
                     /translation="MPKNKSVLIVISIIFWLFLSLYPWQSWLEWTGVLRFLIGLFIYF
                     VPGVLTFMRVSRDGNFSPRIFLGGFVVSVFVTGLLGLLARLLQLNFTFIRWGFALWGA
                     AAVLLFFLMDVKFTFQFEKFTWWEIVSLLLAAGGAIYFASIARPPLIHDDAFTYNALL
                     YYYQHAPALEFQFPVSLDRLEIPRFWIAYWPLVEALISGFSKVDGLIIAGAYLPPALA
                     CFSFLGIYSLGRTLGLPRAAAGIAILAQGFSLMRLTKWNQPGNLFYQRLTEDKVVAAF
                     FISYIFILLAVEYLEKPEARKLILVGVAGLAMVFTHPIQFGMACMIVGVYGLPKLLDK
                     DIRWKYATMIGILALVVLIPYTFRFGGGEYSQTLSFSLDDVAENDEFARFGVRRVDII
                     EGTNFYGISHYLTQGLPYEVSLVSALLCLFFFWKHKHARYVLAAFLVLGVSMFPYTGW
                     IVGMFTTPFQLWRLTWLMPFGMAFAFLIWAGFEISQRFEFVRRLQWLQSVYYLGMTVA
                     MLGLVVYVRPWAMENLPPNGIDMVDLYSNYVSAARQMNEMDVDAPIIIGGPDAATNSI
                     IPSLTLKFEPLVFRVQSGGEQTQLWKPLIGENLSPSERLTRFQENKIEYLLIRDEPAW
                     VVQFQQSFPENVSLVFKDRRFSLYRITP"
     gene            9974..10486
                     /locus_tag="PLE39_11735"
     CDS             9974..10486
                     /locus_tag="PLE39_11735"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNE69045.1"
                     /translation="MYEFDKTDIKIVNLLLEDGRMSASEMSRRMGDISERSIRYRVDR
                     MIDEGVIQISAVVSPEALGFTIKADVWLEVESDLIFEVAKKLASFENVTYVACGIGQN
                     DISIQVVAKDTAEIYYFVTEVVRKVQGVRKTTTSIVPIVLKDVYQWRVPERIAGELSD
                     KESTSTVVSK"
     gene            10505..11803
                     /gene="gntE"
                     /locus_tag="PLE39_11740"
     CDS             10505..11803
                     /gene="gntE"
                     /locus_tag="PLE39_11740"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis PLP-dependent
                     transaminase GntE"
                     /protein_id="HNE69046.1"
                     /translation="MFGSKTQALQQRAQKVLPLGVNSNFRFWGDGITPYVDKTKGGYL
                     WDVDGKKYIDYRMAFGPIILGHAYPEVDSKVIEEIQRGVLFAMTGELEVAVAEMIVEM
                     SPAIEMVRFACSGTEATMHAIRVARAYTGRDIILKFEGNYHGMHDHTLWSTYSPVEAY
                     GNRRSPIAVPASSGIPKSMREFIITLPFNDFEGFERVMRTHGEQIAAVITEPCQGNCA
                     AINPQDGFLQLIRKKTEEYGCVFILDEVKTGFRIANGGAQEYYNINPDMVTYAKALGN
                     GYPIAAFGGKKEIMSIIGHGVAQGGTYTNNKPGVAGAFATLSLLKSKPILKTIEKRGQ
                     RLMDGLKEIFEENEIPAVFTGYPAMFSFSLNVDKVTCQRDWQESDRDLYLKLVEKAVA
                     KGVMPDHDAREPWFLCYEHSDADVEETLNVYAEIVKEVKK"
     gene            11892..13217
                     /locus_tag="PLE39_11745"
     CDS             11892..13217
                     /locus_tag="PLE39_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE69047.1"
                     /translation="MTKLTSKEIVDLSKEYTFFSWSVQGQANPIPVEKAEGIYFWDTD
                     GKRYIDFSSQLMNTNIGHQHPKVVKAIQDQAAKLCFVHPGNTTDVRAQLGKKLSEVTP
                     GNLKKTFFSLGGAEANENAIKIARFYTGRHKILARYRSYHGATHGSIALTGDYRRNAV
                     EPAMPGGVHFLDPFCYRCPFGQKKESCKRECISHLEEVIRYEGPDKIAAIIMEGVVGS
                     NGLIVPPDDYWPRVREICDKYGILLISDEVMSGWGRTGNWFAVDNWGVTPDIITTAKG
                     ITSGYVPLGAVIVSDKIADYFEDKYLYAGLTYNGHALACAAALATIEVYEEDNLIENA
                     RTMGNYLGECLEDIKSRHPSVGDVRYIGLFTTIELVANRETKEIVAPAVMAEVGKVLR
                     ENGLFTFIMANNMGSMVFVVPPLCITKEQLDEGLALVEKALEVADGKIS"
     gene            13284..14750
                     /locus_tag="PLE39_11750"
     CDS             13284..14750
                     /locus_tag="PLE39_11750"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNE69048.1"
                     /translation="MEILNYINGEWVKPSVKDYFDVINPATGQVIAKTPLGTRADVDA
                     AAKAASDALPAWRRTPVNDRVQYLFKLRNLMRENGDEIAKLITNEAGKTFEEAKAEMV
                     RAYENIEVACGMPHMSKGEFVEDIAPGIDEIMIRQPVGVCATIAPFNFPGMIPFWYLP
                     YALATGNTYIVKPSEKVPMTMQFIFKLIEQVGFPKGVVNMVNGAKETVDGILEHPAIR
                     AITFVGSTNVAKYIYSTAAAHGKRVQAQGGAKNPVIILPDADMEMATKIIADSAFGCA
                     GQRCLAVSLAVTVAESKNHFTELICDAAASRTVGYGMDSGVQMGPVINTASQQRVEQL
                     IALGAKEGAGVPVDGRGTVVKGYEAGSFVRPTILSDVKAGSEIARTEIFGPVLSLMHV
                     NTIDDAIQLVNSGQYGNQASLFTTSGNAARRFRYEADAGNIGINIGVAAPMAFFPFSG
                     WKDSFFGDMHGQGTDAVEFFTQKKVVVERWPKEWSRKF"
     gene            14787..16148
                     /locus_tag="PLE39_11755"
     CDS             14787..16148
                     /locus_tag="PLE39_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE69049.1"
                     /translation="MSVDYEDALDYSSRWIDIIQKNEFPSPDEAHKIIDESKNNFAEH
                     YNRNWLEYRKSVTEAGDWAAVEWGGSGAVFKDVLGREYLDFLGGYGMLDLGWSHPDVV
                     NTVKAQLERSPMPSQELIDPLRGVLAKLLASITPGDLKYSWFAASGTEANEAAMKIAK
                     LYTGKTAFIVGVKAFHGKTMGSLSLMGKSDYRAPMGAMYGGQVYHVPFGDAEAVEKQL
                     EICDKVGIGVAAVMFEPIQGESGAIVPPDDFWPRIRAATKKYGVLLIADEVQTGLGRT
                     GKLWGVEHWNVVPDILTVAKSLGGGVMPVSAVTTTEEIFKPMMYPNPFMHTTTTGGGA
                     LACSAAISAIHVTLRERLWEKAAEKGSYLIPQLEKFAAQYPQIYEKITGKGLLIGMHF
                     QSPEIGYKVASGLFKRGVLVAGTLTSAQTIRIEPPLVVTMQQIDMLLDRLGDVLSEIS
                     KTI"
     gene            16178..17293
                     /locus_tag="PLE39_11760"
     CDS             16178..17293
                     /locus_tag="PLE39_11760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE69050.1"
                     /translation="MTSEFAVELRDVVKRFITPEGNEMAAVDHVTMQIKNGEFFSMLG
                     SSGCGKTTSLRMIAGFEWPTEGEVFIEGKPMGHTPPFQRRVNTVFQSYALFQHMTIYQ
                     NVAFGLEMEGAKDEEIKKRVGRALDMVQLTGMDRRKPKQLSGGQQQRVALARALVKTP
                     EVLLLDEPLGALDLKLRKEMQLELKALQQQLGITFIYVTHDQEEALTMSDRIAVMSKG
                     KVQQMGTPVEVYERPANKFVADFIGESNFLEGKIRSISQNEATVFIPGLNAELTGMPV
                     SEGLVKGEEVHVSIRPEKVRVAEKNALNQNMFHAKVTNTVYIGADTHIYVEAHGVKLK
                     VLEQNRISRLDPKSFYSVGQEVWLMLMPENTLILKKD"
     gene            17299..18222
                     /locus_tag="PLE39_11765"
     CDS             17299..18222
                     /locus_tag="PLE39_11765"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE69051.1"
                     /translation="MLQRLRESKSAQGTLLALPTTVWLFVLLIIPLILTLIVSFGRRS
                     PDGDVIYTFSLENYIRLSGFSTDCPDGQASCFDPLYLGILGRSLSLAFNTTLFVITLA
                     YPLAYFIARAHPKQRNMYLFMVLIPLWTNFVIRVYAWMMLLRKEGAINVIIGWVMNLL
                     NIPFTPLEMLYTPGAVLVGMIYEFLPFMILPIYTSLEKIDNSLYEAAADLGANSVKTF
                     LRVTLPLSMPGVVAGTILVFIPVMGTFIVSDILGGRQVILVGNLIQRQFLDARDPTFG
                     SAASLILMVLTLIVTYFYTRKFGFGEEIVAA"
     gene            18219..19115
                     /locus_tag="PLE39_11770"
     CDS             18219..19115
                     /locus_tag="PLE39_11770"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE69052.1"
                     /translation="MNPFRRSPFVIAAATLVYVFLYAPILVLILYSFNASRTNVTFEG
                     FIPSFSDRLEMDGSLVKASPCGPFHWYCDLANNDDVAEAAGNTLTIAFTATIVATIIG
                     TMAALALQRYNFKMKPFSQLSLYIPIVIPEIVMGIGILTLFSQFFGWANEAFNLQGDA
                     RLSLGIVTVTVSHIAFMVPFVTLVVQARLQGFDKSFEEAAMDLGANEWITFRRVTFPM
                     ILPGVLSGALLAFTLSLDDFVITFFTNGPGSTTLPIYVYGLLRRIITPQVNALSTVWI
                     LVVFTAVLLLQILQNRESKQHS"
BASE COUNT         4493 a         4771 c         5199 g         4765 t
ORIGIN      
        1 aaaactggaa ccctaaaatg aacccaatcc caaaacttac atccctcggt caatcccttt
       61 ggtacgacaa cattcagaga aaacttctcg aaaacggcga actcaaggcg atgatcgagc
      121 gcggtgacat ccgcggcgtg acctcgaacc caactatctt tcagaacgcg attgcaaaga
      181 caaccgatta cgatgccgcg ttgattccgc ttgcatgggc aggctgggat gcggaaagga
      241 tcttctggca ggttgctgtt gaagatattc aggaagcctg cgacctcttt cgcccgttgt
      301 acgatgaaac gcgcggcggc gatgggtatg tgagtctcga agtcagcccg tttttggcga
      361 acgacaccga aggcacacag aaacaagcca aggaattgtg ggaacgtgtc aaccgcccga
      421 acctgatggt aaaaatccct gccaccaagg cgggcattcc cgccatccgc agcacgattg
      481 cggcagggat caatgtcaac gtcacattga tcttctctct tgatcgctat gccgctgtga
      541 tggatgccta tctggctggc ctcgaagatc gcatcgccgc aaatgaatcg attgaaatgg
      601 tcgcgtcggt tgcgtcattc ttcgtttcac gcgttgacac aaaaattgat ccgcgtttgc
      661 ccgaaggttc acccctgcgc ggacgcgctg cgatcgcaaa tgcaaaactt gcctacgatg
      721 cttttgagtc tgttttcacc tcgccgcgtt tcgccaccct caaagcccgt ttccgtgcgc
      781 gtgtgcagcg tccgctgtgg gcttccacag gcacgaaaaa ccccgcttac tccgacacgc
      841 tttatgtcga taccctcatc ggcccagaca ccgtcaacac ggtgccgccc gccacgctgg
      901 atgccttccg tgatcacggc aaggccagcc tcaccatcac ccgcggactt gaagaagcgc
      961 agttgttttt tgccgaactc aaagccgcag ggatttcgat gggctccgtt acgcaggaat
     1021 tggaggagga aggtgtcaag gcttttgacg atgccttcaa gactctcttg caagccattg
     1081 aggatcggag gaagagcgcc gtttcttctc ttggaccttt agctgattct gtggccaaac
     1141 gtttgaccac gctggaagaa cactcggtcg ccgcccgcat ctgggcacat gacccgacac
     1201 tttgggcgag tgaccccgaa ggtcaggcag agatcaagat ccgcctcggc tggctgcatt
     1261 ccatcgaaga tgcccgcaca cggttggatg ggtatctctc cttcgcaaaa cagattcacg
     1321 aagaaggcat tgaccgtgtc ctcgtcatcg gcatgggcgg atcgtcactg acggctgaag
     1381 tgctcagctc gttgatggct gcggcgaatg ttgatgcgaa attatccctt gccattctcg
     1441 attcgaccga ttctgagcag gtggctttgg cagcgaagaa ttatccgcca gagaaatcgt
     1501 tgtacattct cgccagcaaa tcaggcggca ccgcagaatt gctggccgcg ttcgattatt
     1561 tctgggaact cagcaagggc aatggctcgc gcttcatggt caccaccgat gcgggcacct
     1621 cgcttgaaaa actcgccaag gatcgcggtt tcagaaaagt attcaacgcc gaccccaatg
     1681 tgggtggacg tttctctgca ttgactgatt tcggtctggt gcctgcggct ttgctcggca
     1741 tggatatcga aaagttgctg gcttctgctg agaagatcaa aaatgcctca cacaagaacg
     1801 tcagcgcggg gtttgcgctt ggcgctctac tggctgagtc tgctctcgca ggcagagaca
     1861 agctcacggt cctgagtgat gcgcctgttt cagcgttggc aggttggatc gagcaggtta
     1921 ttgccgagtc cagtggcaag catggaaagg gaatccttcc cgtgccgatg gagcctttgg
     1981 gttcacccga tgtatatggt gatgaccgcc tgtttgtata tttgcgaaac aacggtgaat
     2041 tgagtgatgg cattgccgcg cttaagaatg caggtttccc cgtcgtcgaa ttgccaattg
     2101 ccaatctcta cgacgcaggc gcggagttct tccgctggga gatcgcgatc tcggtggcct
     2161 gccacatttt gggaatcaac acttttgatc agcccgatgt gcaggatagc aaattgcgca
     2221 cgatcgcgaa gatcaaagat tatcaggcca caggcaaact cgccgaagcg gatctggtgg
     2281 acgtcgccga tgcgaaatct gcgttggaga aattcatgac tggggcgcag gcaggcgagt
     2341 tcttcaccat caatgcctat ctgccgcgca acaaagagac cgagaatgcg ttgcaagcct
     2401 tgcgcgttgc cattcgtgca aagaccaaat gcgctgtttc ggctggcttt ggtccgcgct
     2461 tccaacattc cacggggcaa ttccacaagg gcggccccaa taagggacgt tttattcagg
     2521 tggtgtacga tgcccaaaat gacatggaca ttcccaatca gggcatgacc ttcggcacgc
     2581 tgatccgcgc tcaggcgctg ggtgattacg aggcgttggt ggccgcgggc agaaaagtat
     2641 tgcgtgtgaa gttgaattcg gtggaggatt tgaagaagtt gttgtaaatt tttgttgggg
     2701 tacggcgccg tcgtgcccca accgagttga cagatgaaat acgaccccca agttcatcat
     2761 cggcgttcga tccgtttgaa gggatatgat tacacacaag cagatgcata tttcatcacg
     2821 atttgttcgt atcaacggga gcatgtgttt ggtgaggttg tgaacgggga aatgaaattg
     2881 agcgcatggg gcgagatcgc ccgcgcggaa tggttcaaga ctgcggcatt gcgtgataac
     2941 gtggaattgt atgaagatga atttgtcgtc atgcccaacc atattcatgg gattgtttgg
     3001 ttgcatgagg tagggacacg gcgccgccgt gtccctacgg agaaattcgg aaaacccgtt
     3061 gcgggttcaa taccaaccat catccgcgca tacaaatctg cagtgaccta tgccattaac
     3121 ggattggaaa aatcgcgcgg cggtgcgatc tggcaaagga attattacga gcatgtcatc
     3181 cgcaacgaaa aagaactgaa taccatcgcc agatacattt actacaatcc cttcaactgg
     3241 cagttggatc gcgataatgc aaataacatc cgcaacatcc ccttgcccga aaccattgac
     3301 gactatctgc tggacgctga aaagatggtg ggaataaaag ccttataaat gatcgttttt
     3361 ataaaatcgg ggtgttttct acaaactggc tctgcgtttt ctcaatgcct catcatccag
     3421 ccatttgaaa atccgcagca cgaaatgaaa atctcccagc caactgagca gggatcccca
     3481 caacgctgcc agcccagtgg ggatttgggg taattctgtg gcgggggtga aatccacgtt
     3541 acggttgtca atttcgaagg tgtagcgtcc cgtctgtttg atcgcgggag tcagattcaa
     3601 gtatgcgcgc agatgctcgt actcatcggt tgtcatccac tgcggcaggt ctgaagtggc
     3661 tttgaacttg cagatcagcg cgaggtcttg cagcagggat ttttgttcgg gggtcagcga
     3721 attttcattc gattcaaaat acaccacatc agggtcaatg cgcacgagcg gatcgagcca
     3781 caagcgattg atcaccgtgc ggatggcgtt gcgcgtgcca ggaatggcga agttgtaaaa
     3841 cagcaccgag tcgcggtgtt tttcccagtg tgcgcccaca tcgggtccga tgcgcatcgc
     3901 atcgcacaag ccgagcgcag gcaggatcgg cgtgccacag gtcaggaaga acgcgtccgc
     3961 gcccatcgct tcgcgcatca cgcgcaggct ttcgcgatac gcggcttcgc gcggtatgtc
     4021 gttgtgccgt ttgcctttca acgcgccgcc gtagaggaaa tccagtttga ggtagtcaaa
     4081 gccccaggcg cggacctgct tcatgagcgc aaccagccac ggggttacgt caggatgcgt
     4141 ggtgtcgagc gcgtagagcg gctgtcccca gttgaatccc gccgagacca gttgtccatg
     4201 ctcgtcgcgc agaaaccaat ctgcatgggt gcgaaagagt tgggatgttt ttgtcgcgat
     4261 caacggcgcg agccataacc ccgcccgcct gcccgttgat tttattttct ccgccagcgc
     4321 cgtcatgccc gacggaaatt tttcgttcac cgtccaatcg ccgatgtcca tctgccagcc
     4381 gtcatccact tgcagcactt caaaaggcag gtcggtcaac tcgtcgaaaa ttttatgcag
     4441 cgcgggctca tcgatggcgt gatacaggct gtaccacgaa caccacacgc gcggtgtatc
     4501 atttttttga atggacccga agcgatgttc caacgctttg gcatactcgg caaagatgac
     4561 atcttcccga ccaaaagcca tgaaccactc gacatcgccc gcttcgctcc gccctgtgag
     4621 ttgatcctgt tcaaggagca cggtcgtgtc tgtggcaagc gcgcccaaca aaaggacgtt
     4681 gccatccgcg aattccaccg cgcccagcca cgacccattc ggccgtgact catgcaccgt
     4741 caccgcatcc acctgcagcg gatgaaagat ggcaggcttc tgcacaggca gcggactcaa
     4801 actcgtccat gccgccaacg accacgattg ccagccgtga cgataatatt tcacaggcgt
     4861 tgaaggcagt tcgatctgca actcacgcgc cgcaatgatt tttgattcag tctggatttt
     4921 cgagaagtga attttggtca tattaacctt gcaaaaacaa aggctacgaa ggggaaatcc
     4981 tttgtgtact tagtttcctt cgtgtttaat gcttttggaa attatctgtg cgccaaagaa
     5041 tgccaccccg ccgctgatca acaccgaaaa cagcagcaca aggtgagaac cagccgcaat
     5101 cgccagcgcg gtcttgtatg gatagccaaa tgccaccagc accgaagtcc agccgacttc
     5161 atgcgtgccg atgttggcaa agccttgcaa ggggagcagg gtgaggggga tcatcacgat
     5221 ggaaatgatc gcaatgtgat aaaaggccac aggcagcccc atgctgcgcg tggcaaagta
     5281 gaaattggaa taaacacaca gccagattcc cgctgtgagt agagccaccc gtccatgtgc
     5341 gccgtggttt tgaatctcgc gcaatcccgc gatgaatttt gtccacgccg cacggatgcg
     5401 ggtgatcagc gttttttcgc caactggctg gttgtctgtt gcaggtttgc tgaatctcag
     5461 aaagagcaaa atgccgatgg tgccgagcaa aatcaacaga cagaaaatga ccgatgattc
     5521 aaatatccaa acgggcatat ccttttttga aaacggcagc agaatcgcca gaaccaaccc
     5581 cacgaccgaa aagtcataat aacgggcagc cagtaacgtg gctgtccctt ctgtgaggga
     5641 aaggtccaat ctgtctttcg cgaggaagat atacgccacg tcacctgttt tagcgggcat
     5701 gagatacatc aaaatattat gcagcgccga aacggggacc aactcgatcc acttcacagg
     5761 gcgggaatag atcaaattgg taaagcgcaa tgcccgcaag aaaatgttga gcaaatatgc
     5821 cagaaaagcc atgcccagcc agccccagtg gagatcagaa aacgccacac gcaaaatttc
     5881 ccaatcgagg ttgagcagca aataagcaag taaaacagca gagagaattc cgctgatgac
     5941 gtaaaggagg agattacggt ttttcatcca attaccaatt accaattacc tttttttccc
     6001 attcactctc cccgccatag ccatacttcg ccatccttgc cccgcacaac gcctgcaact
     6061 gcgcagcttg tttcggcgtc caatcgggcc attcgggaaa atcggttttg gatgaggtgt
     6121 tggctggttg acggaatttc tcgcggatac ccgtcacacg cggcaggccg agaaaatctg
     6181 tgattctgcc gaatagatct tcggggttgt cagtgttgaa caagtcttcg aagcgtacac
     6241 gcatgtaggg cgtggatgtg ttttcgaggc cttccatcac gcggttcttg aaatcccaga
     6301 tccagcagta tttttcgaag cgggtgaagc caatagcttt gctgagcggc agttcgccca
     6361 ccaaaaacgg attcggctgc cagaacggga cgaaatagtt ggcgatgaaa ctcgtccctt
     6421 tttggcggga gaagttcaag tgtgaggtca cgtaactgcg cggatcgcgc acgatgtgca
     6481 gaactttcag gtttgggtaa agttcaggcg cgagcggggc cagcccatac aaaaagcaat
     6541 tcgcatcaat gtggaatttc gcttcgcagg tttcaatctc gtttcctttg agcaatttcc
     6601 acgcggtttt cagtccgctt ttggggaaga gatgcgaaaa atgcaaattg gtgaggatct
     6661 ggatcggtct cgaagcgccg cgttcatgat aggaaacaga atcgggatac aggtcggcaa
     6721 agaggcgtga gaaaaaaatc gtccccgtgc gcccagtgga gatgatcaga atcggttggg
     6781 tatctttcat tcgtctttgt ggaggggggt attcgattcg ctgcggtcaa agcgcaattg
     6841 agcgatctgt tcagagacca gccccaccat gaagatcagc gccgcggtgg acatcagcag
     6901 ggcggaggtc tgcccgtagg gggcgttgag gatgaagatg cgcatcaacc cgtatgccac
     6961 gccgagcaca aaagtgaaga tgctggcagg gacgaagatc ttcaacggcg cgtagaaaac
     7021 tgcgatcttc aaaatgatca tcaaaaagcg cagaccgtcg cgcagtggct tgatcttgct
     7081 ttttgttttt ccctcgcgcc gtgcaaagcg gatcgattcg tacccgaggc tgtaccccgc
     7141 gcggacgact gacaatgtaa tggtggtggg atacgaaaat tgattcggca aaagataaac
     7201 gaattggcgc gcaatgtgtg ctttgatggc gcggaaacct gaggtcaggt cttcgatctt
     7261 gcgccccgaa acatacgtgg cgagcgaatt gaaaaccatg ttggctacat cgcgatgcgc
     7321 agccgtgtct gattcacgat tgcggctgcc gacgaccatg tcgtaggggc cgatgcggtt
     7381 cacgagtttg ataatgtcat tggggtcatg ctggccgtca ccgtcaatgg tgacgaggat
     7441 ctgtccacgc gcattgcgaa tcccagtttt gaccgcggcg ccattgccga tgttgtaggg
     7501 atgttcgatc actcttgccc cggcatcgcg cgcactttgg gcggtgttgt cctttgaacc
     7561 gtcgtcaatg acgatgatct cgttgtctgg gtccagtgta ttcaccaccg cgcgaatccg
     7621 cccgatcacg ccgccgatta cctcgccctc attgaaggca gggatgatga ccgtgatctt
     7681 cggtgatgtc tttttttctg taggcattca ttgctccata aaagccgatt actcggagcg
     7741 cggacttcag tccgcgttgt gttatcgtgg cggactgaag tccgcattcc aatcatgatt
     7801 tgcttcccca aaaagcgcgc tgggataata atcgaaacaa gtataattgg ctaggtatcc
     7861 ccaattttag gaagaatcat gcccaaaaac aagtctgttc ttattgtcat ctccattatt
     7921 ttctggttgt tcctctcgtt atatccctgg caatcctggc tcgaatggac aggcgtcctg
     7981 cgttttctga tcggcctgtt catttatttt gtccctgggg tgcttacatt tatgcgtgtc
     8041 agccgcgacg gaaatttttc tccgcgcatt tttctaggcg ggttcgtcgt gtcggttttt
     8101 gtgacggggc tgctcggtct gctggcgcgt ttgttgcagc tcaacttcac ctttatccgc
     8161 tggggatttg cgttgtgggg tgcggctgcc gttctgctat ttttcctgat ggatgtgaaa
     8221 ttcacttttc aatttgaaaa attcacatgg tgggagatcg tttccctgct tttggcggca
     8281 ggtggcgcaa tttactttgc cagcatcgcc cgtccgccgt tgatccacga cgacgccttc
     8341 acctacaatg ccctgcttta ttactaccag catgcacccg cacttgaatt tcaattccct
     8401 gtttcactgg accgtttgga aatcccccgc ttttggatcg cttattggcc gttggtcgaa
     8461 gccctgatct ctggattcag caaagtggat ggactcatca tcgcgggggc gtatcttccc
     8521 cccgctctgg cctgcttctc cttcctgggc atttactccc tcgggcggac tctcggcctg
     8581 ccccgcgccg cggcagggat tgccatcctc gcacaaggct tcagcctgat gcgcctgacg
     8641 aagtggaatc agcctggcaa ccttttttac cagcgcctta ccgaagataa ggtcgttgcg
     8701 gcatttttca tttcatatat ttttattctg ctggcagtgg aatatctgga aaagcccgaa
     8761 gcgcggaaat tgattctcgt cggggtggcg gggttggcga tggttttcac gcatcccatc
     8821 caatttggca tggcttgtat gatcgtcggg gtttatggct tgccgaaact gcttgataaa
     8881 gatatccgct ggaaatatgc aacgatgatc ggaatccttg ccttggtggt tctgattccg
     8941 tataccttcc gctttggcgg gggcgaatat tcgcagaccc tcagcttctc gctcgacgat
     9001 gtcgccgaga acgatgaatt cgcccgtttc ggtgtccgcc gcgtggatat tatcgaaggg
     9061 acgaatttct acggcatttc acattatctg actcaaggtc tgccgtatga ggtcagcctt
     9121 gtttcagctt tgctatgtct tttcttcttt tggaagcata aacatgcccg ctacgtactg
     9181 gcggcatttc tcgttttggg cgtgagcatg ttcccgtaca caggctggat cgtcggcatg
     9241 ttcaccacgc cgtttcagtt gtggcgcctc acctggctga tgccgtttgg aatggcgttt
     9301 gcatttctga tctgggctgg ttttgaaatt tcacaaagat ttgaattcgt ccgccgtttg
     9361 caatggcttc aatccgttta ttatttgggt atgaccgtag ccatgctggg gctggtcgtt
     9421 tacgtccgcc cgtgggcgat ggaaaatctc ccgcccaacg gcattgacat ggtggatctg
     9481 tattccaatt acgtcagcgc ggcacggcag atgaacgaaa tggatgttga tgcgccgatc
     9541 atcatcggtg ggcctgatgc cgccaccaat tccatcattc ccagcctgac gttgaaattc
     9601 gagccgctgg tcttccgtgt gcaatcggga ggggagcaga cccagctctg gaaacccctt
     9661 atcggggaga atctttcccc gtcagaacgc ctgaccagat ttcaggaaaa caagatcgag
     9721 tatctgttga tccgcgatga gcctgcgtgg gtggtccaat tccagcaaag tttccccgaa
     9781 aacgtctccc ttgttttcaa agaccgcagg tttagtttgt atcgaattac gccctaaaca
     9841 ggaacacggg atattatctc gtattactaa ctcggccgtc accttgtgtg gcggcttttt
     9901 tattccccct tgacgattaa aacgccacaa ggtatattct gcgcgtaact ttcggaaggt
     9961 ataaagtgaa tctatgtatg aattcgacaa aacagatata aaaatcgtca acttgttgct
    10021 tgaagatggc cgaatgtctg cttctgagat gtcccgtcgt atgggtgata tctcagagcg
    10081 gtcaattcgg tatcgtgttg accgcatgat cgacgaaggg gttattcaga tcagtgcggt
    10141 ggtcagcccc gaggcgctgg gttttaccat caaggccgat gtctggctgg aggtggaatc
    10201 ggaccttatt ttcgaagtgg cgaagaaatt ggcctctttt gaaaatgtca cctacgtggc
    10261 ttgcggcatc ggacagaacg acatcagtat tcaggtggtt gccaaggaca cggcggaaat
    10321 ctattatttt gtgactgaag tggtgcgcaa ggtgcaaggg gtgcgcaaga ccaccacgtc
    10381 catcgtgccg atcgtcctca aagatgtgta tcaatggcgg gtgcctgaac ggattgcagg
    10441 cgaattatcg gataaagaat cgacatcgac cgtggtatcc aaataaatca gtaaaggaga
    10501 gtgtatgttt ggttcgaaga cgcaagcttt acaacagcgc gcgcaaaagg ttttaccttt
    10561 gggggtgaac tccaattttc gtttttgggg tgacgggatc accccgtatg tggataagac
    10621 caagggcgga tatttatggg atgtggacgg aaagaaatat attgattatc gtatggcttt
    10681 tgggccgatc atcctcggtc atgcttaccc cgaggttgat tcaaaagtaa ttgaagagat
    10741 tcagcgggga gtattgttcg ccatgacggg cgagcttgag gtggctgtcg cagaaatgat
    10801 cgtggaaatg tcgcctgcga tcgaaatggt gcgttttgcc tgttcaggta ccgaagccac
    10861 catgcatgcc atccgcgttg cccgcgcgta caccgggcgc gatattattt tgaaatttga
    10921 aggcaattat cacggtatgc acgaccatac cttgtggtcc acctattcgc ctgtggaagc
    10981 gtatggcaat cgcagaagtc ccattgcagt gccggcctct tcgggtattc ccaaatccat
    11041 gcgcgaattc atcatcaccc tgccttttaa tgattttgaa ggttttgagc gtgtgatgag
    11101 gacacacggc gagcagatcg ccgcggtcat caccgaaccg tgtcagggaa attgcgctgc
    11161 gatcaatccg caggatggat ttttacagct tatccgcaaa aagaccgagg aatacggttg
    11221 tgttttcatt cttgatgaag tgaagacggg cttccgcatt gcgaacggtg gtgcgcagga
    11281 gtactacaac atcaaccctg acatggtcac ctacgccaag gcgcttggaa atgggtatcc
    11341 cattgcggct tttggcggaa aaaaggagat tatgtccatt attgggcatg gcgtggcgca
    11401 aggcggcacg tacacgaaca acaagcctgg tgtggctggg gcgtttgcca ctttgagcct
    11461 gctcaaatcc aagccgattc tcaagaccat cgaaaagcgt ggacaacgcc tgatggacgg
    11521 tttgaaggaa atcttcgaag agaacgaaat ccctgctgtt tttacggggt atcctgccat
    11581 gttctctttc tcgttgaatg tggacaaggt cacctgtcag cgcgattggc aggaaagcga
    11641 ccgtgacctg tacctgaaac ttgtggagaa ggctgttgcc aaaggcgtca tgcctgacca
    11701 cgatgcccgc gaaccctggt tcctttgcta tgaacattcc gatgcagacg tagaagaaac
    11761 cttgaatgtg tatgcggaaa tcgtgaagga agtgaaaaaa taattgttta aaggtttgaa
    11821 ggttgaaaag tttgaaggtt tcgacttgtt aactcttaaa cctgtgtact tgttaaacct
    11881 atggagaatc catgacaaaa ctaacatcca aagaaatcgt tgaccttagc aaggaataca
    11941 cctttttctc gtggtcggta cagggccagg ccaatcccat ccccgtggaa aaggcggagg
    12001 gcatttattt ttgggatacg gatggaaaac gctatattga tttttcatcc caactcatga
    12061 ataccaacat cggtcatcag caccccaaag tggtcaaagc cattcaagat caggcggcga
    12121 agttgtgttt tgtgcatccg gggaacacca ctgatgtccg tgctcagctt ggtaaaaaac
    12181 tctcggaagt gaccccggga aacttgaaaa agacattttt ctccctcggc ggtgcagagg
    12241 cgaatgagaa cgccatcaag atcgcacgtt tttacacggg ccgtcataag attctggccc
    12301 gctatcgttc gtatcacggc gcgactcacg gctccattgc actgacaggc gattaccgcc
    12361 gcaatgccgt tgaacctgcc atgcccggcg gggttcattt ccttgatccg ttctgctatc
    12421 gctgtccgtt cgggcagaag aaggaatcct gcaagcgcga gtgtatttca catctcgaag
    12481 aggtgatccg ttacgaaggt cccgacaaga tcgccgcgat cattatggaa ggcgtggtcg
    12541 gctcgaatgg gctgatcgtt ccgcctgatg attattggcc gcgcgtccgc gagatctgtg
    12601 ataaatacgg cattttgctg atctcagacg aagtgatgag cggatggggt cgcactggta
    12661 actggtttgc cgtggacaat tggggcgtca ctcccgatat catcaccacc gcaaaaggca
    12721 tcaccagcgg atatgttccg cttggcgcag tgatcgtttc agacaagatc gccgattatt
    12781 ttgaagataa atatctttac gctggcctta cgtataacgg ccatgctttg gcttgcgccg
    12841 cggcgcttgc caccatcgaa gtgtacgaag aggataacct catcgaaaac gcacggacga
    12901 tgggtaacta ccttggcgaa tgtcttgaag atatcaaatc acggcacccc tcggtgggtg
    12961 atgtgcgtta cattggcctg ttcaccacca ttgagttggt cgccaatcgg gaaaccaaag
    13021 agatcgttgc tcctgccgtc atggccgagg tagggaaggt actgcgcgaa aacgggcttt
    13081 tcacctttat catggcgaac aatatgggca gcatggtgtt tgtcgttcca ccgttgtgca
    13141 tcacaaaaga acaactggac gaaggcctgg cattggttga aaaagcgttg gaagttgcgg
    13201 atggaaaaat cagttaacaa gttgacaagt tggaaggtct gaacgtttca accttccaac
    13261 tttcaaacgt gaatggagaa ctcatggaaa tcctaaatta cattaacggc gaatgggtca
    13321 aaccctcggt caaagattat tttgatgtca tcaaccctgc cacgggacaa gtaattgcaa
    13381 agacgccgct tggcacaagg gcagatgtgg atgcagcggc aaaggccgcc agcgatgcct
    13441 tgccagcatg gcgcagaacc ccggtcaacg atcgggtgca atacctgttc aaactccgca
    13501 atctcatgcg tgaaaacggc gatgagatcg ccaagctcat caccaacgaa gcaggcaaga
    13561 cctttgaaga agccaaagcc gaaatggtgc gcgcctatga aaacatcgaa gtcgcctgtg
    13621 gtatgccgca catgagcaag ggcgaatttg ttgaagatat tgcccccggc attgatgaga
    13681 tcatgatccg ccagcctgtg ggcgtatgtg ccaccatcgc gccatttaat ttcccgggca
    13741 tgatcccgtt ctggtatttg ccgtacgcac ttgccacggg caacacgtac atcgtcaaac
    13801 cttctgaaaa agtacccatg acgatgcagt tcattttcaa actcattgag caggttggct
    13861 tcccgaaggg cgtggtcaac atggtcaatg gcgcgaagga aaccgtggat ggcatattgg
    13921 aacaccctgc cattcgcgcc attactttcg tcggctcaac caacgtggcg aagtatattt
    13981 actccactgc tgccgcacat ggcaaacgtg tgcaagcgca aggcggagcg aagaaccctg
    14041 tcatcatttt gcctgatgca gatatggaaa tggcgacgaa gatcatcgcc gattctgcct
    14101 tcgggtgtgc tggtcagcgc tgtttggcag tttccctggc tgtgacagtt gccgagtcaa
    14161 aaaatcattt caccgagttg atctgcgatg ccgcggcatc tcgcaccgtc ggctacggca
    14221 tggattctgg cgtgcagatg ggcccggtga tcaataccgc gtcacaacaa cgcgtggagc
    14281 aattgatcgc attgggtgca aaggaaggcg ctggtgttcc ggtggatgga cgcgggacgg
    14341 tggtgaaagg ctacgaggct ggatccttcg tcagaccgac gatcctctcc gatgttaaag
    14401 caggaagcga aatcgcacgg acggaaatct tcggaccggt gctcagtctc atgcatgtca
    14461 ataccatcga cgatgccatc caattggtca acagcggaca gtatgggaat caggccagct
    14521 tgttcaccac cagcggcaat gctgcacgcc gtttccgcta tgaagcggat gcgggcaata
    14581 tcggtattaa tatcggtgtg gctgccccga tggcgttctt cccgttcagc ggctggaaag
    14641 attctttctt cggcgatatg cacggacagg gcaccgatgc ggtggaattc ttcacccaaa
    14701 agaaggttgt ggtcgaacgt tggcccaagg aatggtcgag aaaattttaa tgtaactcaa
    14761 acctaagttt gcgttacgga ggatgtatgt cagtagatta cgaagatgca ttggactatt
    14821 catctcgctg gatcgatatc attcaaaaga atgagtttcc cagcccggat gaggcgcaca
    14881 agatcattga tgaatcaaag aataattttg ccgagcacta caaccgtaac tggttggaat
    14941 atcgcaaatc agtgacagag gccggggatt gggccgctgt ggagtggggc ggttccgggg
    15001 ctgtattcaa ggatgtcctc gggcgcgagt atctggattt cctcggtggc tacggcatgt
    15061 tggacttggg ctggagccac ccggatgttg taaatacggt caaggcgcag ttggaacgtt
    15121 cgccgatgcc gtcacaggaa ttaattgatc ccctgcgcgg tgtgctggca aaacttttgg
    15181 cttccatcac ccccggtgac ctgaaatata gctggtttgc tgccagtggc actgaagcca
    15241 atgaagccgc gatgaagatc gccaagttgt acacaggcaa gacggcattc atcgttggtg
    15301 tgaaggcatt tcacgggaag acgatgggct cactttcctt gatgggtaaa tccgactacc
    15361 gtgcgccgat gggagcaatg tatggcggcc aggtttatca cgttccattt ggcgatgcgg
    15421 aggccgtgga aaaacaactg gagatttgcg acaaggtcgg tatcggtgtg gcggcggtga
    15481 tgtttgaacc gatccagggt gagtcggggg cgattgtgcc tcccgatgat ttttggccgc
    15541 gcattcgcgc cgctacaaag aaatatggtg tgttattgat cgccgacgaa gtgcagacgg
    15601 gtcttggccg caccgggaaa ttgtggggcg tggagcattg gaacgtcgtc cccgatattt
    15661 tgacagtggc gaagtcgctt ggcggcggtg tgatgcccgt cagcgcggtg acgaccacag
    15721 aagaaatatt caagcccatg atgtatccga atccttttat gcataccaca acaacgggcg
    15781 gcggcgcttt ggcttgttct gcggcaattt cggccattca tgtaaccctg cgtgaaagat
    15841 tgtgggaaaa ggcggctgaa aagggttcgt acttgattcc acaattggaa aaattcgcgg
    15901 cgcagtatcc gcaaatttat gagaaaataa caggcaaagg cctgctgatc gggatgcatt
    15961 tccaaagccc ggagatcggt tataaagttg cttcggggct tttcaagcgt ggagttctgg
    16021 tggctggaac gttgacaagc gcccaaacca ttcgcattga accgcctctg gtcgttacaa
    16081 tgcagcaaat cgatatgctg ctggaccgcc ttggcgatgt tttaagtgaa ataagcaaga
    16141 caatttgata ttccatactc atctgaggag aagatagatg accagtgaat ttgcagttga
    16201 attgcgcgat gttgtaaaac gttttattac ccccgaaggg aacgaaatgg ctgccgtgga
    16261 ccatgtgacc atgcagatca agaatggtga gttcttttcc atgcttggct cttcagggtg
    16321 cggcaagacc acgtccttgc gtatgatcgc agggttcgag tggcctaccg aaggggaggt
    16381 gttcattgaa ggaaaaccga tggggcatac accgcctttt cagcgcagag tcaatacagt
    16441 atttcagagt tatgctttgt ttcagcatat gaccatttac cagaacgtcg cttttggtct
    16501 ggaaatggaa ggtgcaaaag acgaagagat caaaaaacgt gtcgggcgtg ccttggatat
    16561 ggttcagctc actggcatgg atcgccgcaa gcccaaacaa ctctctggtg gtcaacagca
    16621 gcgtgtggcg cttgcccgtg ccttggtgaa aacccccgaa gtgcttttgt tggatgagcc
    16681 tctcggcgcg ttggatttga aattacgcaa ggaaatgcag cttgaattaa aagcactgca
    16741 acaacagctt ggcatcacct ttatctacgt tacgcacgat caggaagaag ccctgaccat
    16801 gtcggaccgt attgcagtga tgagcaaggg gaaagtacag caaatgggta caccagtgga
    16861 ggtgtacgaa cgccctgcca acaagttcgt ggcagacttc atcggggagt caaacttcct
    16921 cgaaggcaag atcaggtcca tctcacagaa tgaggcgacg gttttcatcc ccggtttgaa
    16981 cgccgagttg accggtatgc ctgtctctga aggccttgtc aaaggcgaag aggttcatgt
    17041 aagcattcgc cctgaaaagg tgcgtgtggc tgagaaaaat gcattgaatc agaatatgtt
    17101 ccatgcgaag gttacgaaca cggtttacat tggcgcagat acccatattt acgtggaagc
    17161 acatggtgtg aagttaaaag tgcttgagca aaaccgtatt tcacgccttg accccaaatc
    17221 tttttactcg gtggggcagg aggtttggtt gatgctcatg cccgaaaata cgctgatttt
    17281 gaaaaaggat tagcagccat gcttcagcga ttacgtgaaa gcaaatctgc acagggcacg
    17341 cttcttgcat tgcccaccac ggtgtggttg tttgtgttgt tgatcattcc gttgatcttg
    17401 accctgatcg tcagttttgg caggcgcagc cccgatggag atgtgatcta caccttctcg
    17461 ttggaaaact acatccgcct gtcaggtttt agcaccgatt gccccgatgg gcaggcttcc
    17521 tgttttgacc cgctgtattt gggtatttta ggccgttcac tttcattggc gttcaacacc
    17581 actttgtttg tgatcactct tgcctacccg ttggcgtatt ttattgcccg tgctcacccg
    17641 aagcagcgca atatgtacct gttcatggtg ttgattccct tgtggaccaa ctttgttatc
    17701 cgtgtgtatg catggatgat gctgttgcgc aaggaaggcg cgatcaatgt catcattggc
    17761 tgggtgatga atctgctgaa tattccattc acgccgcttg aaatgcttta caccccgggc
    17821 gcggtattgg tgggaatgat ctacgaattc ctgccgttca tgatcctgcc gatttacacc
    17881 tcgcttgaaa agatcgataa ttctttgtac gaagccgccg ccgatctggg cgcgaattct
    17941 gtcaaaacct ttttgcgggt cactttgccg ctgtccatgc cgggcgtggt ggcagggaca
    18001 attctcgtct tcatcccggt gatgggaact ttcatcgttt ccgatattct tggcgggcga
    18061 caggtgattc tcgtaggtaa cttgatccag cgccagttcc tcgatgcacg cgacccgaca
    18121 tttggttccg ccgcttcgct tattttgatg gtgcttaccc tgatcgtgac ctacttctac
    18181 actcgtaaat tcggatttgg tgaggagatt gtggccgcat gaaccctttt cgccgttccc
    18241 cattcgtgat tgccgcggca accctggtgt atgtcttttt gtatgcgcca atcctggttt
    18301 tgattttgta ctccttcaac gcatcgcgga caaacgtcac ctttgaaggg tttatccctt
    18361 ccttcagtga tcgccttgaa atggatggca gccttgtaaa agcgagtccc tgcggaccgt
    18421 tccactggta ttgtgatctg gccaacaatg atgatgtggc ggaagctgct ggcaacaccc
    18481 tgaccattgc tttcacagca acgatcgttg cgaccatcat tggtacaatg gctgccctcg
    18541 cccttcagcg ctacaacttc aaaatgaagc cgttttcgca gttgtcgctt tatatcccca
    18601 tcgttatccc tgagatcgtg atgggtattg gtattttgac cttgttcagc cagttctttg
    18661 gttgggcaaa tgaagccttc aacctgcagg gtgatgcgag gttgtccctt ggtattgtga
    18721 cggtgacggt gagtcacatt gctttcatgg tgcctttcgt gacactggtg gtgcaggccc
    18781 gtcttcaggg ctttgacaaa tcctttgaag aagccgccat ggatcttggc gccaacgagt
    18841 ggatcacctt ccgccgtgtg acttttccca tgatcctgcc gggggttctc tcgggcgcct
    18901 tgctggcctt caccctttcg ctggatgact ttgtgatcac cttctttacc aatggaccag
    18961 gttctacaac cctgccgatc tatgtctatg gtttgttgag aaggatcatc actccgcagg
    19021 tcaatgcgct ttcaaccgtt tggattttgg tcgtttttac ggctgtattg ctgttgcaaa
    19081 ttttgcaaaa ccgtgaatcg aaacaacatt cctaaactta attgggtatt ccgtgcttcg
    19141 aaaagcgcgt gccaaatgac ttcaatactt ctaaggagga agagatgagt agaaagaacc
    19201 tatatggatt actgggggtg ttggtcat
//
LOCUS       DAOQJC010000072        19225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1311_length_19225_cov_8.594366, whole genome shotgun sequence.
ACCESSION   DAOQJC010000072 DAOQJC010000000
VERSION     DAOQJC010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 19225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19225
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1311_length_19225_cov_8.594366"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="PLE39_11775"
     CDS             complement(<1..1402)
                     /locus_tag="PLE39_11775"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNE69053.1"
                     /translation="MIKKLLKKSERGQAIILIAFAFVGLIAMIGLMTDGGVLLIEYAR
                     LKRGIDAASVAAAQQFRKDFNGDDLVSAAENFLKLNQSDVIDVTISMCDPTNAYGGSP
                     AHDASLCPDPGQPARKLVRVTATRNVDFGFMRVLGINGTTIQATSVGEAASIDLVLIM
                     DTSLSMAYGTDGNPISGSKDDNPAACNAAGNCQPLENIKDVAREFVSEFMFFPYDRVA
                     VVTMTSQTPGGTRDPILFPFEDDATDIDNILRDIKVYEPPLCVRSATNPWDRQWLSPS
                     KGVCLEYDSLGVYSGVNSPIRRGGVDLNESTLADNLNDPTTTGSSNIGGSLYYANNAF
                     IQSARQDAFWVVIMLAGGPANATSTDDVVHYPDGFCPQTAWKTQCRDGDLAADGVTPL
                     ITRHNRNTASNPPTYPVAYDADDYARDAADDLANPETGNGITIFTIGLGTSSGIANSL
                     QHNGLPTYPDGTLVPNG"
     gene            complement(1421..2047)
                     /locus_tag="PLE39_11780"
     CDS             complement(1421..2047)
                     /locus_tag="PLE39_11780"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HNE69054.1"
                     /translation="MKSFFSKLLPPLRTDAPRKRKTLGQSLIEFAVALPLLIGILVAM
                     MEFGFILNFYLSLLDATRGAARWASLANPLNADGTDDMDYYITTAELVRTELAPPTDG
                     RKLILDPATDGVIVTVYCVVGGTTATRYPDTGPYNTNPSMITSAFTTADIENTYADTA
                     PDAGLVLVEVYYGYRPVIGLFYTGKVMLRAHSIMPANAADPIPGTGCQ"
     gene            complement(2351..3613)
                     /locus_tag="PLE39_11785"
     CDS             complement(2351..3613)
                     /locus_tag="PLE39_11785"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HNE69055.1"
                     /translation="MNRKLAANFRNSERGQAIIVIVFAIIGIVGISSLAIDGSNALID
                     RRKSETAASAAALAGALARIEGRDWRESALAAAKMNGYDNDGENSIVELNTPPINGPY
                     AGDAEYIEVIVTSNMRTYFGSIIGIPVVTSVGYAVAQTKPAEYGQMFDGYALVSLAPH
                     SECDKKKAFWLHNEPTIVLEGGGIFVNSDNDVCAFLQEGSGSIRVQDESPITVVGGAS
                     VQKAQLISPAIVQPGAIPISYPPAFQMPKIGCGSKIATIDLVTGTMTSGNYEEPGDFP
                     PEGIHDLESGIYCISGDVVIGGGTRLTGNGVTLIVEQGEVRIAAEAEVDLSAPKNGNA
                     AGLLIYMPLDNHSRIALNGNADSNFTGTILAPSADIRLNGPDSKHGFHSQIIGYYIEV
                     DGQSKIVIKYEDEDNYDAYKMPEVILVQ"
     gene            complement(3610..4074)
                     /locus_tag="PLE39_11790"
     CDS             complement(3610..4074)
                     /locus_tag="PLE39_11790"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNE69056.1"
                     /translation="MKVKADHRERGQSLVELAVSLPIILLLLLGTLDFGMAIFSYSML
                     RDAAQEGAFYGSFNPASKEAIENRARYISPRGEDEIFSSPVDLTNPDLVQVEVDTLGQ
                     ACQGITDETINSIRVTVTYNYPIMMPIIGPLFGSTIPLTGTASNIILQPACP"
     gene            complement(4090..5232)
                     /locus_tag="PLE39_11795"
     CDS             complement(4090..5232)
                     /locus_tag="PLE39_11795"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HNE69057.1"
                     /translation="MKRFIFSKNNKTRAQAMVEFMLALPILLIILYGLIEVGRLAFIF
                     SSTANASRQAARYGAASGEINDVAFYQDCEGIRSVAEESAFIIEFENINITYDRGVDE
                     DGNQVPISGVDPSPDEDTCPIPPGVIRNGDRIIVQVSAIYEPIISGPVEPMEIVSSSA
                     RSFLISVPIVGSALPTGFSPETSTPSQVPTSSLFTSTPTLYVSPTYTFIPPSLLTNTP
                     NPNFTPTLTREPTLTFTPSRTPLPTYTPSVTPTAISCNGITGVTHGPLEFKDNVMKMS
                     IDNQTGHTLVAANIYLEWNHDTGHGEGNDLGLILNRVTFAGQAWDGALQAPSAYIPAF
                     YPTIPPGISTISFSFHQNYLLTDGTERIIVNIGTPGCINYPVDSSH"
     gene            complement(5342..6130)
                     /locus_tag="PLE39_11800"
     CDS             complement(5342..6130)
                     /locus_tag="PLE39_11800"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNE69058.1"
                     /translation="MSITAIISILFGWLAGVLVNYLADVLPHTRRFSQPACLHCNAPF
                     AWQDYLLFKSCPNGHARSTRTWVVQFVILAISIYIWLNPPSKIGYGLGMILLVYFGIV
                     FVIDLEHRLILHPTSIFGALFGLIIGTIAHGIAPTLWGGLGGFVIMMVLYYFGVLFAR
                     VRTRRLIAAGQEPDDEEALGAGDVILITILGFILGWPLIWFGFLLGVLLGGFFSLLII
                     VWLVVVRKYRQNAMMMFIPYGPYFVTSAFLVVYFPKFLALLVPG"
     gene            6261..7541
                     /gene="murA"
                     /locus_tag="PLE39_11805"
     CDS             6261..7541
                     /gene="murA"
                     /locus_tag="PLE39_11805"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNE69059.1"
                     /translation="MEEFVIEGGTPLHGEVTPSGNKNAALPLLAACLLTSEPVVLKNV
                     PQIRDVQVMRKLIESLGSQVEDLDSNTWRITTKDLVASHLDPDLCRRIRASILIAGPV
                     VARAGTLRLPPPGGDVIGRRRLDTHILALRALGAKVTYDRVFEFEADQLHGADILMDE
                     ASVTATENAIMAAVLAKGQTQIRNAASEPHVQEVCQFLNILGAKIEGIGSNTLHITGV
                     PQLKGGEFTIGPDLLEVVSYVGAAVVTRGEIRIRNAGVQHLEMVGQVFHRLGVHWNVE
                     GNDLFVPADQSLVIASDLDGAVPEIKTNVWPAFPTDLMSIAITVATQASGSVLFHDWM
                     FSGRMYFTDKLVGMGARIILCDPYRVLIQGPTRLYGEKLESPDIRAGMALLLAALAAK
                     GTSKIRNISQIERGYEKVDEKLRMLGASITREQE"
     gene            complement(7628..8242)
                     /locus_tag="PLE39_11810"
     CDS             complement(7628..8242)
                     /locus_tag="PLE39_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69060.1"
                     /translation="MLKRILPILLALSVLLTACGQATPTAAPVDVEGTAVAAAWTMVA
                     ATQMAIPTATPLPPTETPAPTVPPTFTPPPLLELPTATLFNLPTPTAVSAADDCNHPL
                     NVGEAGETNPMRIENASGGTIAWLSLNLSKNAFGQCGAMSWSNIGNGAKFKVDLPKGI
                     WWAYAGITFKNGSSSNASGSFEIRLGDEDLLRIIVGKDVITVHP"
     gene            complement(8277..8969)
                     /locus_tag="PLE39_11815"
     CDS             complement(8277..8969)
                     /locus_tag="PLE39_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69061.1"
                     /translation="MKKYFFSFFVLGFALSACMPALPAFFQSEPTSSPIPLPTSEENL
                     QATAAVIAQQTLEALPSPTLEPSHTPIVPTQTSTSTQQPTPTETVNPILLTLTATLGT
                     GTVSPGTPGTPGASTGTPNPAFSATPTETLHPQLYGTMPPSLPSGYVTFINESRAEVY
                     VSLQCTTKEGFVTIIEYPVGRRVSTDAPAGEYIYVVWVGGNKITGEFRLGASQTLTMT
                     IFKDRVAIKSKL"
     gene            complement(8999..9343)
                     /gene="trxA"
                     /locus_tag="PLE39_11820"
     CDS             complement(8999..9343)
                     /gene="trxA"
                     /locus_tag="PLE39_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNE69062.1"
                     /translation="MSSEPIHVTDEAFEKVVMQSTLPVIVDFWAPWCNPCKMIAPTLE
                     KIAVEQEGKLVVAKINTDEHPQWMQKFGIQGIPTLLFISGGKVVHRQVGALPERMLRD
                     VVNQFMEVISNN"
     gene            9487..10398
                     /gene="ftsY"
                     /locus_tag="PLE39_11825"
     CDS             9487..10398
                     /gene="ftsY"
                     /locus_tag="PLE39_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNE69063.1"
                     /translation="MNNLFSRWKESLTRSSKAAFGQLASILGTSEITEETWDDLEALL
                     LQADLGLETTSSVLDSLKRITRTEGLVRANELSATLRQELRARLSTPPALSLNDKPSV
                     VLVVGVNGSGKTTTIAKLSSRFTAEGRKLLLGAADTFRAAAVDQLQVWGDRLKVEVIA
                     GAFESDPGAVAFNAVQAGVARGVDIILIDTAGRLHTRFNLMEELKKVNRVVGKALPGA
                     PHQVWLVLDATTGQNALQQARSFKEAVQVSGVILSKLDSSARGGMAFAIQRELGLPIL
                     FAGLGEKPEDLQPFDPDAFVDGILSNA"
     gene            10407..11499
                     /gene="prfB"
                     /locus_tag="PLE39_11830"
     CDS             join(10407..10472,10474..11499)
                     /gene="prfB"
                     /locus_tag="PLE39_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114679478.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNE69064.1"
                     /translation="MQDLLNRLQAANDLTQQLMVRLDLAGKEIQLEQLEKDIGQPEFW
                     NDSIGAQKVMKTVSSLRDEVGFWNSTKQHLHDLTDLVSLGDESLRNDLEGEIAKLEAS
                     LDKKSFNTMFSGPFDHDDAILAIHAGAGGTDSQDWAGMLQRMYLRWAESQGFGTEVLD
                     FTPGEEAGIKSITIAVSGEYAFGHLRSEKGVHRLVRLSPFDAAHRRHTSFALIEVLPQ
                     VSMEEADVEINPSDVKIDVFRSSGAGGQNVQKNATAVRLTHLPTGIVVTCQNERSQSQ
                     NREFAMKILRARLLELKTAEKEEERAILRGEYTKAEWGSQIRSYVLHPYQMVKDHRTD
                     HETGNSQAVLDGDLNGFMEAFLKSSFPEG"
     gene            11636..>11982
                     /locus_tag="PLE39_11835"
     CDS             11636..>11982
                     /locus_tag="PLE39_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69065.1"
                     /translation="MSVFSVGVLAFAAFIVGRVGRGRELTLLAASVFFIYWLQPAQTI
                     SSLSFWLPTITLALSIFCWVLVAPPEIRVWGKTWPAVLVIVGTILFVDLSQFLGSKIF
                     SATPRLWIVVLALA"
     assembly_gap    11983..12082
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12083..12868
                     /locus_tag="PLE39_11840"
     CDS             <12083..12868
                     /locus_tag="PLE39_11840"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE69066.1"
                     /translation="LAEYLNYVIFFPSFAAGPIDRAERFVLELRNPVALAEQDWVQAG
                     TRFFIGLFKKFVIADALALVSFNDALAEQVRSPVWIWFLLYAYSLRIYFDFSGYTDIA
                     IGLARILGIRLPENFAAPFLKPNLTQFWNSWHMTLTHWFRAYFFNPITRSLRTKQLPV
                     PLIIFTAQLSTMVVIGLWHGITWSFFFWGIWHGLGLFIQNRWSEWIQSRMETPPPPHR
                     QLVLNVIGMFLTFNFVSLGWLFFTLSSPLLALQVLMKLFGAII"
     gene            12865..13983
                     /locus_tag="PLE39_11845"
     CDS             12865..13983
                     /locus_tag="PLE39_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69067.1"
                     /translation="MKVNPLRVLVKALILYVVANILFAIYNPPIGRISVYNWLVPGRL
                     RFPYERAENVNLGYNISVFQDLDAAFASHVIADGEKPADEYRVILVGDSSIWGFASNS
                     TETLSAKLNDLELTACDGRRMRFYNLGYPWSFIFKDLLVLEHARVYEPDMVLWLFTMR
                     GLTGSDKDIVDFFEGELIVDAKRVQAKYGLEDYTSELTTQTFFEQTIIGERKKIKTDI
                     TLQMYGFLWAATGIDHHLQTWLPWKDTLENSIEYRHYRTPEEWPQLQDDFMLDVLDAA
                     HTMMGDTKIVAVNEPIYISSGSNSEVRYNKLYPRWAYDRYREFMQGWMAENQYPYLDM
                     WNVIPQEEFSDFPVHLYPTGERLLAESLAPEVLKFSCP"
     gene            complement(14031..14813)
                     /locus_tag="PLE39_11850"
     CDS             complement(14031..14813)
                     /locus_tag="PLE39_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNE69068.1"
                     /translation="MTTPDSAPAVALEPKTKLSGKILKTTLAGALVDIGQSLPGVIHI
                     SQLQQDAVNKVEDVVKEGQTVEVWVRRVKKDRIELTMIEPLGYEWKEIVPDMTVKGKV
                     VRIETYGAFVDFGAERPGLIHVSELTHGYVKTAGEVVKEGDEVEAKVLDVDRRKRQIR
                     LSMKALLPEIVEEEKPEREERSKKGDKPKGKKGKKQEEEAYEMEVEVSNEPQFTAMQI
                     AWQQALERSKGRGKVRTKSYKSASDEKEELFNRTLEKRASSN"
     misc_binding    14957..15240
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15298..18450
                     /gene="ileS"
                     /locus_tag="PLE39_11855"
     CDS             15298..18450
                     /gene="ileS"
                     /locus_tag="PLE39_11855"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNE69069.1"
                     /translation="MFKAVNPKLNVPLMEETILKLWKKQNVFKKTVEQRKDGPEYVFY
                     EGPPTANGRPGVHHVLARAFKDMFPRYKIMRGYHVSRRGGWDTHGLPVEIEVEKQLGF
                     TNKQQIEEYGIDKFNELCRNSVFTYIQDWEKLTDRIAFWVDLETAYVTYENEYIESVW
                     WILKNFWEKDLLFKGYKIVPYCPRCGTPLSDHEVALGYDDAVDPSVFVRMPLVDKPDT
                     SLLVWTTTPWTLPANVAVAAHPEVDYVTIERDNDGTKEKLILAKNLVEKIFAGEEVKV
                     VDSFKGKKLKGLKYKPLFTFVPVDKPAYFVVLGDFVTTEDGSGLVHQAPAFGAEDMEM
                     AKQHDLPILLTVQPDGTFIPEITPWRGIFVKDADPQIIQDLDARGLLFRAGTLTHTYP
                     FCWRCKTPLLYYARESWYIRTSAKRDRLVNLNKTINWVPDHIKNGRFGNWLENNIDWS
                     LSRERYWGTPLPVWECENQECKHRECVGSVAELSEKAGKDLTELDLHRPHVDNVHWKC
                     VECGGKMSRVKDLIDVWFDSGSMPYAQWHYPFENQDKFKQQFPADYICEAVDQTRGWF
                     YSLHAISTLLNDDVSFKNVICLGLILDGEGRKMSKSIGNIVNPWDVLNVHGADAFRWY
                     LYTASPAGQERRFSSDLVGDVIRSFTLTLWNVYSFFVTYANLDKPQALTITAPTNDLD
                     RWLLSELNVLVRDVTNAYENYDATNATRPVEKFVETLSTWYVRRSRRRFWKNDSDSDK
                     QAAYSTLYTALVTVAKLIAPAMPFVAEELYQNLVRSVDETAPESVHLAEWPKVMEEFI
                     DESLNRDMNLVMKLVSLGHAARQKANRKVRQPLAEAAFSVGNNVERNALSANMDVIAD
                     ELNVKQVRLLDASTEALTHTVKPLPKQLGQKYGNKFPALQKAILAMNAEEVAKILHSG
                     APLNVTVDGETYGILSDEVEVKALAKEGFAVAEDGAYVAALVTDLTPELIAEGQAREF
                     VRRVQDLRKTADLDVADRVELFIEATAGLRSAIEAHEGYIKAETLTVKLSFANPPATA
                     SVVEDEFDGEKVKVGLVKSK"
     gene            complement(18631..>19225)
                     /locus_tag="PLE39_11860"
     CDS             complement(18631..>19225)
                     /locus_tag="PLE39_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69070.1"
                     /translation="EAFIYPPQYPNVQLRFDKMKNLLKTGTTQIPELDGDKPVTINLR
                     ELLDGFVTPQERRKDEEREKQEGKRGDTYITNNNITMGNMHGSSLTLGDGNTSTVSVQ
                     NSFNTFPAELQNTLTELMKASETLLQKAEESEHTEEIKDELESLQTEAKKSKPKKERI
                     KVTVEGLAQAAKNLNEIGKPVLELAMTVIKLINNLPA"
BASE COUNT         4535 a         4492 c         5161 g         4937 t
ORIGIN      
        1 gaccatttgg aacgagtgtg ccatctgggt aggtaggcaa accattatgc tgaagcgagt
       61 ttgcaattcc tgatgaagtt cccaagccaa ttgtgaatat agtaatgcca tttcccgttt
      121 ccgggttggc aaggtcatca gcggcatcac gggcatagtc atcagcatcg taagccacag
      181 ggtaggtggg aggattggag gcagtattac ggttatggcg cgtgatgagt ggtgtgaccc
      241 catctgctgc aaggtcacca tcacggcatt gagtcttcca agcggtttgt ggacagaagc
      301 cgtcaggata atgtacaaca tcatctgtgc tggttgcatt ggcaggaccg ccagcaagca
      361 tgattacaac ccaaaatgca tcctggcgag cagactggat aaacgcattg ttcgcgtagt
      421 acaatgaacc accaatatta cttgaaccgg ttgttgtggg gtcgtttagg ttatctgcta
      481 atgtagactc atttaaatca acaccgccgc gtcttattgg gctattaacg cctgagtaaa
      541 caccgagaga gtcgtattcc aagcatacac ctttgcttgg agatagccat tgccgatccc
      601 aaggattcgt agcactacga acacacaatg ggggctcata aacttttatg tcccttaaaa
      661 tattatcaat gtcggttgca tcatcttcaa atggaaacaa gatggggtca cgagttccgc
      721 caggagtttg tgaagtcatg gtaactacag ccacccggtc gtaggggaag aacatgaatt
      781 cagagacaaa ctcacgtgct acatccttaa tattttcaag tggctgacaa ttccccgccg
      841 cattgcaagc ggctggatta tcatccttac tacctgagat tgggtttccg tctgtaccat
      901 atgccattga gagggatgtg tccataatca aaaccagatc gatggatgct gcttcgccta
      961 cagatgtggc ttgaatggtg gtgccattaa tccctaaaac ccgcatgaac ccaaaatcca
     1021 cgttgcgtgt ggcagtcaca cgcaccagct ttcgggcggg ctggcccggg tctgggcata
     1081 gggatgcgtc atgggcagga gaaccaccat aggcattggt tggatcgcac atggagatcg
     1141 taacgtcaat aacgtctgat tggtttagtt tgagaaaatt ttcggcagca ctaacaagat
     1201 catctccgtt aaagtctttg cggaattgct gggcagcagc tacagaagcc gcgtcaatac
     1261 cgcgctttaa gcgagcatat tcgatcagca agacgccacc atcggtcatc aagccgatca
     1321 tagcgatcaa gccaacaaat gcaaaggcga tcaggatgat ggcctgacca cgttctgatt
     1381 ttttgagcag ctttttgatc ataaattcac tctctcttct tcactggcac cccgtgcctg
     1441 gaatagggtc agctgcatta gctggcatga tggaatgcgc gcgtagcatc accttccctg
     1501 tatagaacag gccaatcact gggcggtacc cataataaac ttcaacaagt accaatcccg
     1561 catcaggagc ggtatcagca taagtgtttt caatatctgc tgtagtgaaa gcagatgtaa
     1621 tcatgctagg gttggtatta tatgggccag tatctggata acgagtggct gttgttccac
     1681 cgactacgca atacactgta acaataacac catctgtagc aggatcaaga attagtttgc
     1741 gaccatcagt tggcggggcc aattcggttc ttaccaattc ggcggtggtg atgtagtaat
     1801 ccatgtcgtc ggtgccgtct gcgttaagtg gatttgcaag gctggcccag cgtgcggctc
     1861 cacgggtggc atccaaaagc gacagataga aattcagaat aaaaccgaat tccatcatgg
     1921 cgaccagaat accgatcaac aacggcaacg caactgcaaa ctcaatcaaa ctttgaccaa
     1981 gggttttgcg tttgcgcggt gcatcagtac gtaaaggcgg aagtagtttt gaaaaaaacg
     2041 atttcatatt attctcgata atagattatt ttagtgctta ttcaatcaaa atatacatcc
     2101 gacataagta cgggaaaaca aacatctttg gctgtgatgc tgggggaatg aaaagcgcgt
     2161 tttttggcgt tttttcgatg caaatacatt atatctcatt caaaagacgc ctgtcactat
     2221 gcaagaatgc tgcaacgcta acagatttgt aagaacaagt cagttttgga gctatttccc
     2281 cgtgaatctg acttttgtag gctgaaaaac ttttacacca aggactgcaa acgtaaggct
     2341 gaaagctcgc ttattgaacc agaatcacct ccggcatctt ataggcatca tagttgtctt
     2401 catcttcata tttgatgacg atcttactct gaccgtccac ttcaatgtag tagccgatga
     2461 tctggctatg gaagccgtgc ttggagtccg ggccattcag gcggatatct gccgaagggg
     2521 caaggatggt gccagtgaaa ttggagtcgg cattgccgtt cagtgcaatg cggctgtggt
     2581 tatcgagcgg catgtagatc aatagaccag ccgcgttgcc gttttttgga gcggataaat
     2641 ccacctcagc ttcagccgca atacgaactt ctccctgctc gacgatcaac gttacaccat
     2701 ttccagtcag gcgggttccg cctccgatga ccacgtcacc actgatgcag taaatgccgc
     2761 tttcaagatc gtgaatgcct tcaggaggga aatcaccggg ttcttcgtag ttgccagagg
     2821 tcatcgtacc ggttaccaga tcaatggtgg cgattttcga accgcatccg atcttgggca
     2881 tttggaaggc tggcggatag gaaatgggaa ttgcacccgg ttgcacgatg gctgggctga
     2941 tcagttgggc cttttgtaca ctggctccgc ctacaacagt aattggactt tcatcctgca
     3001 cacggatgct gccgctgcct tcctgtaaaa aggcgcagac atcgttatct gagttgacaa
     3061 agatgccgcc gccttccaaa acgatggtgg gttcgttatg cagccagaag gcttttttct
     3121 tgtcacattc actgtgcggt gcaaggctga caagcgcgta gccgtcgaac atttggccat
     3181 actctgcggg tttggtctga gccactgcat acccaacact ggtgacgacg gggattccga
     3241 tgatggaacc aaaataggtg cgcatattcg atgtcacgat gacttcgatg tactcggcat
     3301 cccctgcata ggggccgttg atggggggag tgttcagttc cacaatgctg ttctcgccat
     3361 cgttgtcata accgttcatt tttgcggcag caagggcaga ctcgcgccaa tccctgcctt
     3421 cgatgcgggc gagggctcct gccaaagcgg cggcagaggc ggcggtttca gactttcggc
     3481 ggtctatcag ggcattgctt ccgtcaatgg cgagggagga tatgccgaca atgccaataa
     3541 tggcgaacac gatcacaatg atcgcttgtc cgcgttcgct atttctaaag ttggctgcta
     3601 attttcggtt catgggcatg cgggttggag gatgatgttc gaggctgtac cagtaagcgg
     3661 gatggtggat ccgaataatg gtccaatgat cggcatcatg atcgggtagt tgtatgtcac
     3721 ggtgacgcga atgctgttga tcgtttcatc tgtaataccc tgacaggcct gcccaagtgt
     3781 atccacttcc acttgaacaa gatcggggtt ggtgaggtct accggagagg agaatatttc
     3841 atcttcaccg cgcggtgaga tgtagcgggc gcggttttca atggcttctt tgctggcagg
     3901 gttgaacgag ccgtagaagg cgccttcctg ggccgcatcg cgcagcatgg aatatgaaaa
     3961 gatcgccatg ccaaaatcca gcgtgcccaa taacaacaaa aggatgatgg gcaggctgac
     4021 ggccagttca accagacttt gtccgcgttc gcggtgatct gccttcactt tcatggaagt
     4081 tgaaatcctt tagtgactcg aatccactgg gtagttgata caaccagggg tgccgatatt
     4141 cacgataatg cgttcggttc catcggtcag aagataattt tgatggaagc tgaaactgat
     4201 cgtggatatt ccaggtggaa tggtcgggta aaaggcgggg atgtaagcag atggtgcttg
     4261 gagtgcgcca tcccaggcct gacctgcaaa ggtaacccgg ttgaggatca aaccgaggtc
     4321 gtttccttca ccatgacctg tgtcatgatt ccattcaaga tagatatttg ccgcaaccag
     4381 tgtgtgaccg gtctggttgt cgatgctcat cttcataaca ttatctttga actcgagcgg
     4441 accatgtgtt acgccggtga tgccgttaca actgattgcc gtgggggtga cgctgggcgt
     4501 gtaggttggc aggggtgtgc gtgacggtgt aaaggtcagg gtgggctcgc gggtaagcgt
     4561 gggagtgaaa ttcgggttgg gtgtatttgt cagcagtgaa ggcggaataa aggtataggt
     4621 tggagaaacg tacaatgtgg gagtggaggt gaacaggctg ctggtcggca cctgtgaagg
     4681 agtgcttgtt tcaggagaaa acccggtagg cagcgctgac ccaacgatgg ggacagagat
     4741 caggaaggag cgcgcgctgg aagacacgat ttccatgggt tcgacggggc cagaaataat
     4801 cggctcgtat atggcagaca cttgcacaat gatgcggtca ccattgcgaa tgacgcccgg
     4861 gggaatgggg caggtatctt cgtcggggct tgggtcaaca cccgatatcg gaacctggtt
     4921 tccgtcttcg tccacgccgc gatcgtaggt gatgttgata ttttcaaatt cgatgataaa
     4981 ggccgattct tcagccacac tacgaatgcc ttcgcaatcc tgataaaaag cgacatcgtt
     5041 gatctcgcct gaggcagccc catagcgggc agcctgacgg gatgcatttg cagtggatga
     5101 aaagatgaat gccagtcgcc caacctcgat caacccatac aggatgatca gcaaaatggg
     5161 taaagcaagc atgaactcaa ccattgcttg tgcgcgggtc ttattatttt tggagaagat
     5221 aaatcgtttc atatagccag cttttcatct aacttgattt tggggcgggg gatttgtgtg
     5281 tacgtctaat tttatattac tggtttgggc cgtcaaaagc catcggtcta agttcctatg
     5341 gctatccggg cacaagcagc gcgaggaatt ttggaaaata gacaaccaaa aaggcgcttg
     5401 tcacaaaata tgggccatag ggaatgaaca tcatcatggc attttgcctg tactttctaa
     5461 caacgaccag ccagacaatg atcaacaaac tgaaaaagcc gccaagtaaa acacccagga
     5521 ggaaaccgaa ccagatcagc ggccagccaa ggataaaacc aagaatggtg atgaggatga
     5581 catctcctgc gccaagcgct tcctcgtcat cgggttcctg cccggcggca atgaggcggc
     5641 gtgtcctgac ccgggcgaac agcacaccga aataatacaa aaccatcatg atcacaaatc
     5701 cgccaagtcc gccccatagg gtgggcgcaa ttccgtgcgc gatcgtgccg atgatcaacc
     5761 caaatagggc tccaaaaata ctggtggggt gaagaatgag gcggtgttcc agatcgatca
     5821 caaagacaat gccaaaataa accagcaaaa tcattccaag cccatagccg atcttggatg
     5881 gcggattaag ccagatgtaa atactgatcg ccagtatcac gaattgcaca acccaggtac
     5941 gggtgctgcg ggcatgtccg tttgggcagg acttgaataa taaataatcc tgccaggcaa
     6001 atggagcgtt gcagtgcaga caagctggtt gactgaaacg gcgtgtatgt ggcaggacat
     6061 cggctaggta gtttacaagc actccagcaa gccatccgaa cagtatggat atgattgcag
     6121 taatagacat ttcaaatgat tataattcca attgatacca tcgtccaaaa ccccttttgg
     6181 tgtaagtgaa ttgtaatgtt ttcttgaaat gttttatact acctttcaat tctgaaggcg
     6241 ttctgaggag accatatcaa atggaagaat ttgtgatcga aggtggaacc ccacttcatg
     6301 gcgaggtaac tccctctgga aataagaatg cagctttgcc cctgctggca gcttgcctgc
     6361 tcacatctga gcctgttgtt ttgaaaaatg tgccgcagat tcgtgatgtg caagtcatgc
     6421 ggaaattgat cgagagtctt ggttcacagg tggaggattt ggattcaaat acctggcgca
     6481 tcacgacgaa ggatctagtc gcatcacatc tggacccgga cctgtgtcgt cgaatccgcg
     6541 cttcgattct catcgccggg ccagtggttg ccagagcagg aaccctgcgt ttgccacctc
     6601 caggcgggga tgtgatcggt cgccgacgtt tggacacaca cattctggct ttgcgtgctt
     6661 tgggcgcaaa agtgacctac gaccgtgttt ttgaatttga agcggatcaa ttgcatggtg
     6721 ctgatatcct tatggatgaa gcaagcgtta ctgcaactga aaatgccatc atggcggctg
     6781 tgcttgccaa gggccaaacc cagattcgca acgcggcatc cgaaccgcat gttcaggaag
     6841 tctgccagtt tttgaacatt ctgggggcga agatcgaagg cattgggtca aataccctgc
     6901 atatcacggg tgttcctcaa ttaaaaggcg gcgaatttac cattggcccc gaccttctgg
     6961 aagtggtcag ttatgtgggg gcagcggttg tcacccgtgg tgaaattcgc atccgcaatg
     7021 ctggggttca acatttggaa atggttgggc aggtctttca tagattgggc gtccactgga
     7081 atgtggaggg aaatgacctg tttgtccccg ctgaccaatc ccttgtgatt gcctctgatc
     7141 ttgatggtgc tgtgcctgag atcaaaacca atgtctggcc tgcttttccc accgatttga
     7201 tgagcattgc catcacagtg gctacccagg cttctggttc ggtgcttttc catgattgga
     7261 tgttttcggg ccgaatgtat tttacggata aactggtcgg catgggggcg cggatcattt
     7321 tgtgtgaccc gtatcgggtg ttaattcaag gcccaacaag gttgtatgga gaaaaacttg
     7381 aaagccctga tattcgtgcc ggcatggctt tactgctggc tgccttggcc gcaaaaggga
     7441 cttcaaagat acgcaatatc agccagatcg aacgcggcta cgaaaaagtg gatgagaaac
     7501 tccgcatgct tggtgccagc atcacccgtg agcaggaata agaaatgaac aaaaaagaag
     7561 ccgcgaaaaa ctcgcggctt cttttttgtt ttggggaata cggactgaag atccgcactt
     7621 tgcaggatta cgggtgaact gtgatgacgt ctttcccaac gataattctc agcaggtctt
     7681 catcgccaag gcggatttca aaagagccag aggcgttact gctggagcca ttcttgaagg
     7741 taataccggc gtaagcccac cagataccct tgggcaggtc aaccttgaat tttgccccgt
     7801 tgccaatgtt gctccaggac atggcgccgc actgaccaaa ggcgttcttg gaaagattca
     7861 aagacagcca ggcaatggta ccgccgctgg cattttcaat acgcatcgga ttggtttcgc
     7921 cagcttcacc cacattcaaa ggatgattgc agtcatctgc ggcagaaacc gcggtggggg
     7981 tgggcaggtt aaacagagta gcggtcggca gctccaacaa tggcggaggg gtgaatgtgg
     8041 ggggaacggt tggagcaggt gtttctgtag gaggcagggg ggttgcagta gggatcgcca
     8101 tctgggtcgc agcgaccatt gtccatgcgg cggccacggc tgtgccttcc acgtctacgg
     8161 gggccgctgt gggtgttgct tgcccacagg ccgtcaacaa cacggaaagc gcgagcagga
     8221 tgggtagaat tcttttcaac attttttctc catttatttt ttgttttgaa taggacttat
     8281 aacttggact tgatggcaac tcgatctttg aagatggtca tggtcagcgt ttgtgaagcg
     8341 ccgagacgga actctccagt gatcttgttt ccaccgaccc acaccacata gatgtactca
     8401 ccggctgggg catcggttga aaccctgcgc ccaacggggt actcaatgat cgtcacaaaa
     8461 ccttccttgg tggtgcattg caatgagaca tacacctcgg ctcttgattc gttgatgaag
     8521 gtaacgtatc cggaaggcag gctgggcggc attgtaccat acagctgcgg gtgaagcgtt
     8581 tcagtcgggg tggcactgaa agcggggttg ggtgtacctg ttgacgctcc gggagtcccc
     8641 ggtgttcctg gggatactgt gcctgtgcca agggtggcgg tcagtgtaag cagaatgggg
     8701 ttgaccgttt ctgtgggcgt cggctgttgt gtggaggtgc ttgtttgggt tggtacgatg
     8761 ggggtgtggc tgggttcaag agtaggggaa ggcagggctt caagggtttg ctgggcaatc
     8821 actgcagcag ttgcctgaag gttttcctca gaggtcggca ggggaatggg gctgctggtt
     8881 ggctctgact gaaaaaaagc aggcagggca ggcatgcacg cgcttaaagc gaagccaagc
     8941 acgaagaacg aaaaaaaata ttttttcatg gcctgtttcc ttgatcctgc tcccccaact
     9001 aattattgct gatcacttcc ataaactggt ttacaacatc gcgtaacatt cgctcgggta
     9061 atgcaccaac ctgacgatgc accactttgc cgcctgaaat gaacagcagg gtggggatgc
     9121 cctgaattcc gaacttttgc atccattgcg ggtgttcgtc tgtgttgatc tttgccacaa
     9181 ccaatttgcc ttcctgttcc acggcaattt tttcaagcgt gggggcgatc attttgcatg
     9241 ggttgcacca cggcgcccaa aaatccacga tcaccggcag ggtggattgc attacaactt
     9301 tttcgaaggc ttcgtctgtg acatgaatgg gttctgaaga cattgttatt tttattcctt
     9361 tcaacctttc gtcttttgtg tgcatgtgaa aatgtcagtc aacatgcaac acagctgccc
     9421 atagggacaa aaaagtataa cataaaatcc atgatatata atgaccgtag ggtataatcg
     9481 ccgctcatga ataacctttt ttcacgttgg aaagaatcac ttacccgttc gagtaaggct
     9541 gcatttggtc aactcgcatc cattcttggt acatctgaga tcacagaaga aacatgggat
     9601 gatctggaag ctctgctgct tcaagcagac ctgggattgg agaccacctc ctcggttttg
     9661 gattcactta aacgcatcac acggaccgaa ggactggttc gcgccaatga actttcagcg
     9721 acccttcgac aggaattgcg agcccgcctt tccaccccac ccgctttaag cttgaatgac
     9781 aaaccctctg ttgtgttagt ggttggtgtg aacggttcag gcaaaacaac caccattgcc
     9841 aaactctcgt ctcgttttac cgccgaaggc cgaaaactcc tgctcggcgc cgcagatacc
     9901 ttccgtgccg cagcggtgga ccaacttcag gtttggggtg acaggctcaa ggtcgaagtg
     9961 atcgcagggg cttttgagtc cgatcctggt gcggtggctt tcaatgccgt tcaggctgga
    10021 gtcgctcgag gtgtggatat catcctgatc gatacagctg ggcgcttgca cacccgtttc
    10081 aatttgatgg aagaactgaa gaaggtcaac cgtgtggtgg gcaaggcttt gccgggggcg
    10141 ccgcatcaag tgtggctggt gttggacgca accaccggtc agaacgcttt gcaacaggcc
    10201 cgttcattta aagaagcggt gcaggtttca ggcgtcatcc tctcaaaatt ggattcgtca
    10261 gcacgtggcg gcatggcttt tgccattcaa cgcgaacttg gcctgccgat cctcttcgct
    10321 ggtttgggcg aaaagcccga agatttacaa ccctttgacc ccgatgcctt tgtggacggg
    10381 attttatcga acgcgtagga gctattatgc aagacctttt gaatcgcttg caggccgcga
    10441 atgacctgac tcaacaactc atggtgcgtc tttgacttgg cgggcaaaga aattcaactt
    10501 gaacaactcg aaaaagacat aggacaaccc gagttttgga acgattcgat cggcgcgcaa
    10561 aaggtgatga aaaccgtttc gagcctgcgc gacgaagtgg gattttggaa ttcgacaaag
    10621 cagcatcttc acgacctgac cgacctcgtc agccttggtg atgaatcatt acgcaatgac
    10681 ttggaaggtg agatcgccaa acttgaagcc agcctcgata aaaaatcgtt caatacgatg
    10741 ttctcagggc cgttcgacca tgatgatgcc atccttgcca ttcatgcagg agcaggtgga
    10801 accgactctc aggattgggc tggcatgctg caacgcatgt atttgcgctg ggccgaaagt
    10861 cagggatttg gcaccgaggt gctggatttt acccccggcg aagaagcagg tatcaaaagc
    10921 atcaccattg ccgtaagcgg agaatatgcc ttcgggcatt tgcgttctga aaaaggcgtg
    10981 catcgtttgg tgcgtctttc cccatttgat gcggcgcatc gcaggcatac ttcttttgcg
    11041 ttgatcgagg tcctgccgca ggtttcaatg gaagaggctg atgtagagat caatccaagc
    11101 gatgtaaaga tcgatgtctt ccgttcctcg ggtgcaggcg gacagaatgt acaaaagaac
    11161 gctactgccg tgcgcctgac ccatttaccg acgggcatcg tggtcacttg tcagaacgaa
    11221 cgctcgcaat cgcagaaccg cgaatttgcg atgaagattc tgcgcgcccg cctgctcgaa
    11281 ttgaaaaccg ccgaaaaaga ggaagagcgt gccattttgc gcggcgagta caccaaggct
    11341 gagtggggaa gccagattcg ttcgtatgtg ttgcatccat accagatggt aaaagaccac
    11401 cgtaccgacc atgaaacagg taattcccag gctgttttgg atggcgacct gaacggtttt
    11461 atggaggcct ttctgaaaag ctccttcccc gaagggtaaa gcgatgctgc caaaattgct
    11521 ccggaagatc ttctcccgtg aaaattggct tgccctcctt ttgtgcctga tcctgatcgc
    11581 gttgaccatt ttctcagcag acgcgactcc tacgtggatt tatcagggtt tctagatgtc
    11641 tgtatttagt gtcggcgtgt tggctttcgc cgcattcatt gtagggcggg ttgggcgagg
    11701 gcgtgaactg accttgttgg cagccagtgt tttttttatc tactggcttc agcctgccca
    11761 aaccatttcc agtctatcgt tctggctgcc gacgattacc ctggccctgt ccatcttttg
    11821 ctgggtgctg gttgcgccgc ctgagattcg tgtttggggg aaaacctggc ctgcggtgct
    11881 ggtgatcgta gggacaatcc tgtttgttga cttgagtcaa ttccttggtt cgaaaatttt
    11941 ttccgccacg ccgcgtttgt ggatcgttgt gctggcgctg gcnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnctggcaga atacctgaat tacgttattt ttttcccatc
    12121 cttcgcggca ggtcccattg acagggctga gcgattcgtg cttgaactaa ggaatcccgt
    12181 tgctttagcc gaacaggatt gggtacaggc agggacgcga ttttttatcg gcctgttcaa
    12241 aaaatttgtc atagccgatg cgttggcgct ggtctctttt aacgacgccc tcgcagaaca
    12301 agtccgctct ccggtgtgga tctggtttct tttgtatgca tattccctgc ggatctattt
    12361 tgatttcagc ggttacaccg atattgccat cggccttgcg cgtattctgg ggattcgctt
    12421 acctgagaat tttgctgcgc cttttttgaa acccaacctc acccagtttt ggaactcatg
    12481 gcacatgacc ctgacgcatt ggttccgtgc ctactttttc aatcccatca cgcgttcgtt
    12541 gcgcacgaaa caactccctg tgccgttgat tattttcact gctcaacttt caacgatggt
    12601 ggtgatcggt ttatggcatg gcatcacctg gagttttttc ttttggggga tctggcatgg
    12661 cctcggcctt tttattcaga atcgctggag cgaatggatt caatcaagga tggaaacccc
    12721 gcctcctcct caccgacaac tggtactgaa tgtgatcggc atgttcctca cttttaattt
    12781 tgtctcgctg ggctggttgt tctttacgct ctcttcaccg ctgcttgcgt tgcaagtgtt
    12841 gatgaaattg tttggagcca taatatgaaa gtgaatccct tacgtgtgct tgtcaaagcg
    12901 ctgattttgt atgtagttgc gaatattttg tttgcaatct ataacccgcc gatcggaagg
    12961 atttctgttt ataactggct tgtgccgggg cggcttcgtt ttccatatga acgggctgag
    13021 aacgtcaacc ttggctataa catttccgtc tttcaagacc ttgatgcggc ttttgcctct
    13081 catgtcattg cggatggaga aaaaccagcg gatgaatacc gtgtgatcct ggtgggtgat
    13141 tcctcgattt ggggtttcgc ctcaaattcc accgaaacgc tctctgcaaa actcaatgac
    13201 cttgaattaa ccgcctgcga tggcaggcgg atgcgttttt ataaccttgg atacccttgg
    13261 tctttcatat ttaaagacct gcttgtactt gaacatgctc gggtttatga gcctgacatg
    13321 gtgttgtggc ttttcaccat gcgaggcttg acaggctcag ataaagatat agtcgacttc
    13381 tttgaaggtg aattgatcgt tgatgccaag cgggttcagg caaagtacgg ccttgaggat
    13441 tatacaagtg agttgaccac tcaaaccttt ttcgagcaaa cgatcatcgg agagcggaag
    13501 aaaataaaaa cagatatcac cctgcaaatg tacggctttt tgtgggccgc cactgggatt
    13561 gaccatcacc tgcagacctg gcttccatgg aaagatacgt tggagaacag cattgaatat
    13621 cggcattatc gaacccccga agaatggccg caattgcagg acgatttcat gctcgatgtg
    13681 ctcgatgctg cgcacacaat gatgggtgat acgaagatcg tagctgtaaa tgagcctatc
    13741 tacatttcaa gcggatcgaa cagcgaggtc cgctacaaca agctgtatcc gcgttgggcg
    13801 tatgatcggt atcgggaatt tatgcaagga tggatggcgg aaaatcaata tccgtatttg
    13861 gatatgtgga atgtcattcc gcaggaagag ttttcagatt tccccgttca tttgtatccc
    13921 acaggggagc ggctgcttgc agaatccctt gcccctgaag tgttgaaatt ttcctgcccg
    13981 taggtggatg aatcaaaaga gcgagtggca tcactcgctc tttgggttta ttagttgctg
    14041 gaagcccgtt tttcgagagt tcggttgaac agctcttcct tttcgtctga agcagactta
    14101 taggatttgg tgcggacttt gccgcgaccc tttgagcgtt caagcgcctg ctgccaggcg
    14161 atctgcatgg cagtgaattg aggctcgttg gaaacttcca cttccatttc gtaagcttct
    14221 tcttcctgct tcttgccttt cttgcctttg ggtttgtcac ccttcttgct gcgttcttcg
    14281 cgctcgggct tttcttcctc aacgatttcg gggagaaggg ccttcatgct cagacggatc
    14341 tggcgcttgc ggcgatctac atccaaaacc ttcgcttcaa cttcatcgcc ttctttgaca
    14401 acttcgccag cggtctttac ataaccgtgt gtcagttcgc tcacgtggat gagaccaggg
    14461 cgttcagcgc caaagtcaac aaatgcgcca taagtttcaa tgcggacgac tttgccttta
    14521 acggtcatgt cgggtacgat ttctttccat tcgtaaccga gcggctcgat catggtcagt
    14581 tcaatgcggt ccttcttgac gcggcggacc caaacctcaa cagtttgtcc ttcctttacc
    14641 acatcttcca ctttgtttac tgcatcctgc tgaagttgtg aaatgtggat tacaccggga
    14701 agactttgtc cgatgtccac aagcgccccc gcgagagttg tcttcaaaat tttgccgcta
    14761 agtttggttt ttggttcaag cgctacggcg ggtgcgctgt cgggtgttgt catagttata
    14821 ctcctactat ttgaaataat gccgaaaaaa gattatacct gtccgacaac aaatcgtcaa
    14881 taagaagttg ggagaagtta cttcattgat atctgggtct tgccgattgg ttgtgacctg
    14941 tgtataatcc gaaaaatcaa atgtgttgat aagaacagta gattcaccag aaatgttgtc
    15001 agcgagcctg tggttggtgt gagacaggtc aacagggtag atcgaagatc ctcttggagc
    15061 atcaaactga aagcagaaaa gaagcggatg gttttcatcc aaagcttggc tgggagtaag
    15121 ggagacggaa accgccgtta tacgggaaca acctcagtga ttgtgtttgc tggcgttgaa
    15181 tgagcgcctg ctttgacagc aggaacttgg gtggtaccgc gagattctct cgtcccaata
    15241 tgtggacggg agtttttgtt taaaagaatt tggccgatgg ccggtcagga gatgattatg
    15301 tttaaagctg taaacccaaa acttaatgtg ccattgatgg aagagaccat cctcaagctg
    15361 tggaagaagc agaatgtgtt caagaagact gttgagcagc gcaaggacgg ccctgaatat
    15421 gttttctatg aaggtcctcc aactgcgaat ggacgtcccg gcgtacatca cgtgttggcg
    15481 cgcgctttca aggatatgtt ccctcgttac aagatcatgc gtgggtacca cgtttcacgc
    15541 cgcgggggat gggacacgca tggcttgcct gttgagatcg aagttgaaaa gcaacttggg
    15601 ttcaccaaca agcagcagat cgaggaatac ggcatagata aattcaatga actgtgccga
    15661 aactcggttt ttacctacat tcaggattgg gagaagctca ccgaccgcat cgcgttttgg
    15721 gtggacctcg aaaccgcgta tgtgacctat gaaaatgaat atatcgaatc ggtgtggtgg
    15781 atcctgaaaa acttctggga aaaggacctg ctgttcaagg ggtacaagat cgttccgtat
    15841 tgcccgcgtt gcggcacgcc tctttctgac catgaagttg ctcttgggta cgatgatgcg
    15901 gtggacccgt cggtgtttgt ccgcatgccg ctcgtggata agccagatac ttccctgttg
    15961 gtgtggacaa ccacaccgtg gactctccct gccaatgtgg cggttgctgc acatccagaa
    16021 gtggattatg tcaccatcga gcgtgacaac gatggcacga aggaaaagct catccttgcg
    16081 aagaatttgg tcgaaaagat cttcgcgggt gaagaagtga aggtagtgga ctccttcaag
    16141 ggtaaaaagc tgaagggttt gaaatacaaa ccactcttta cttttgtgcc cgtggataaa
    16201 cccgcctatt tcgtggtgct gggtgatttt gtcacgaccg aagatggttc agggcttgta
    16261 catcaggctc ctgctttcgg tgcagaagac atggaaatgg cgaaacagca tgacctgccc
    16321 attttgctca cggtgcagcc tgacggcacc tttatccctg agatcacccc atggcgcgga
    16381 atcttcgtca aagacgccga cccgcagatc atccaggacc tcgatgcgcg tggactttta
    16441 ttccgcgcgg gcacgctcac gcatacctat cctttctgct ggcgttgtaa gactccgctt
    16501 ttgtattatg cccgcgagtc ctggtacatc cgcaccagtg caaaacgtga ccgcctcgta
    16561 aatttgaata agaccatcaa ttgggtgcct gaccatatca agaatggacg attcggtaac
    16621 tggttggaga acaatatcga ctggtcgctc agccgcgaac ggtattgggg cacgccgctc
    16681 cctgtttggg agtgtgaaaa tcaggaatgt aaacaccgtg agtgcgttgg ttcggtggct
    16741 gaactttctg aaaaagctgg caaagacctg actgaactcg acctgcatcg cccacatgtg
    16801 gataatgtcc actggaagtg cgtggagtgc ggcgggaaga tgtcgcgtgt gaaagacctg
    16861 atcgatgtgt ggttcgactc gggatccatg ccctatgccc agtggcatta cccgtttgaa
    16921 aatcaggata agttcaagca gcaattccca gcggattaca tctgcgaagc cgtggaccag
    16981 acccgtggtt ggttctattc gctgcatgcc atcagcacct tgttgaatga cgatgtgtcg
    17041 ttcaagaatg tgatctgcct tgggctgatt ttagatggag agggacgtaa gatgtccaaa
    17101 tccattggca atatcgtcaa tccatgggat gtgttgaacg ttcacggggc agacgcattt
    17161 cgctggtatt tgtacaccgc ctcgcctgcg gggcaggagc gtcgcttctc ttccgatttg
    17221 gtcggggacg tgatccgcag tttcacactt acgttgtgga atgtgtattc gttcttcgtc
    17281 acttatgcca accttgataa gccacaggca ttgaccatca ctgccccgac gaacgatctg
    17341 gaccgctggc tgctctctga gttgaatgtg ctcgtccgtg atgtgacgaa tgcctatgaa
    17401 aactatgatg cgaccaatgc cacccgcccc gtggaaaaat ttgttgaaac cctttccacg
    17461 tggtacgtac gccgctcacg ccgccgcttc tggaagaatg attcggattc ggacaagcag
    17521 gccgcctatt ccacgcttta cactgcgttg gtgacagttg cgaagttgat cgcgcctgcc
    17581 atgccttttg tggctgaaga gttgtaccaa aaccttgtcc gctctgtgga tgagacagcg
    17641 cctgaatcgg tgcatttggc agaatggccg aaggtgatgg aagaattcat tgacgagtcg
    17701 ctgaaccgcg atatgaatct ggtgatgaag ctggtgtcgc ttggacatgc cgcccgtcag
    17761 aaggcgaacc gcaaagtgcg tcagccgttg gccgaagctg ccttctcggt tgggaacaat
    17821 gtcgaacgta atgcgctttc tgccaacatg gatgtgatcg cggatgaatt gaatgtaaaa
    17881 caggtgcgcc tgctcgatgc ctccaccgaa gcgttgacgc acaccgtaaa accgctgccc
    17941 aagcagttgg gacaaaaata tggcaacaag ttccccgccc tgcaaaaggc gatccttgcc
    18001 atgaatgccg aggaagttgc gaagatcctg cattcgggtg cgccgttgaa tgtgaccgtg
    18061 gacggtgaaa cctatggcat cctttccgat gaagtggaag tgaaggcatt ggcgaaggaa
    18121 ggatttgccg tggctgaaga cggtgcctat gtcgccgcgc tcgtcactga cctgacgcct
    18181 gaactcatcg ccgaagggca ggcacgtgaa tttgtccgcc gcgtgcagga cctgcgcaag
    18241 accgccgacc tagacgttgc cgatcgtgtg gaactgttca tcgaggccac agcaggtttg
    18301 aggtccgcga ttgaagcgca cgagggttac atcaaagccg aaacgctgac ggtcaaactg
    18361 tcctttgcga atccgcccgc caccgcatct gttgttgaag atgaattcga tggcgagaag
    18421 gtgaaggttg ggctggtgaa gagtaagtga gttgggaatg ggggataggg aatagggagt
    18481 agtgaatagt gaatagggaa tagggaagcc ctcggaggtc atgagacttc cgagggcttt
    18541 tataaaactt ccatttcaag ttctccccca aattcgacag cactatcgtc gtatttgggg
    18601 gagatgtccg aaggacagag ggggtgcgcc ttatgcgggc aaattattga tcagtttgat
    18661 aaccgtcatt gcaagttcca gcacgggctt gccgatctca tttaaattct ttgcagcttg
    18721 ggccagccct tccacagtga ccttgatccg ttcctttttg ggtttggatt ttttcgcttc
    18781 ggtttgcagg ctttccagtt cgtctttgat ctcttcggtg tgttcggatt cctcggcttt
    18841 ttgcaggagc gtttcggatg ccttcatcaa ctctgtcaga gtgttttgca attcggcagg
    18901 gaaggtgttg aagctgtttt gcacgctgac agtggatgtg ttgccgtcac ccaaagttaa
    18961 cgaactgccg tgcatgtttc ccattgtgat gttgttgttc gtgatgtagg tgtcgccgcg
    19021 tttgccttcc tgcttttcgc gctcttcatc tttgcggcgt tcttggggag tgacgaaacc
    19081 atcgagcaat tcgcgcaaat taatggtaac gggtttgtcg ccgtccaatt caggtatttg
    19141 agtcgtgcct gtcttgagta gatttttcat cttgtcaaaa cgcaactgca cattggggta
    19201 ttgcggcggg taaatgaagg cttcg
//
LOCUS       DAOQJC010000073        19143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1319_length_19143_cov_9.712018, whole genome shotgun sequence.
ACCESSION   DAOQJC010000073 DAOQJC010000000
VERSION     DAOQJC010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 19143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19143
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1319_length_19143_cov_9.712018"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PLE39_11865"
     CDS             complement(<1..615)
                     /locus_tag="PLE39_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69071.1"
                     /translation="MSKKVFTVLLLAVFVLAQFSVASAAAVAGADITLTNTSEFLSEA
                     LAAPWASTTFEWTLGALPAGTTYYVDIYARNAGVNTYASSGECLNVATVNGGVAASGS
                     FTYDFTSTTADGDTWEFVMTVDDTASCADGANVETANVVMHSAYVDGHSINGFGAVPP
                     SDPAIVSDWVACNIFEYWAVGSDAYGLPASTPYSGIGDWNPQITG"
     gene            complement(829..2244)
                     /locus_tag="PLE39_11870"
     CDS             complement(829..2244)
                     /locus_tag="PLE39_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNE69072.1"
                     /translation="MSKKVFTVLLLAVFVLAQFSIASAAQVTADGTLTGPDFMPAGAG
                     STVTLEWTATGLPAGTYVDIYARLMGSGNTYDVAECNNVAFINGSSNGSGTFDYVFTG
                     GGVYGNPADGEYWEFLIAFDDAASCTTLPANANVDANFSASSYVDGTELEGILVIPHP
                     DFTAAGDVACNTFEYWALASDTYGLANPSDPYSGFGSWNAKTTGTFAPAPIGAEDELL
                     SWKITLPATASGLWQFSIDPEDAAGNLNAGPYYFRNSNVSADELEDCADFSDVAGHEH
                     EVYIRYLADLGLISGFTDGTFGPDNTLTRAEASALIEISNGYDATTLPTSAPAGCDFT
                     DVAATDWFAGWVWQACDDGFMNGVGGGLFDPNNLLTRGQIVTIFNNIYELGLGNLQPG
                     SHMVVGQGLLAFDDAAPRYRDAAWSDVSLGAYYAEPVVEAYSWGIVDGTSATTFSPDQ
                     AMTRGEFAKTLYRILMYFGAF"
     gene            complement(2411..3037)
                     /locus_tag="PLE39_11875"
     CDS             complement(2411..3037)
                     /locus_tag="PLE39_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69073.1"
                     /translation="MLIPIHQEMTREALEGRVSPRALEVIIAANCKQDALSGQIGHDE
                     FHYDNNAIDEGHRYVIEQRGFVIASLLSPGVLSAWIAFGRLTHTVQDFYSHTNYVTMW
                     LDAHPGGTPPTPPEIDPVQKDLIESPSLHSGKLYMPMDWLYFVPFLRKLSLSLLPADS
                     HAKMNLDSPVQGAKFAYARAAAVKRTIYEFDLLEKLLTPEMFSKFVDM"
     gene            3104..3646
                     /locus_tag="PLE39_11880"
     CDS             3104..3646
                     /locus_tag="PLE39_11880"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNE69074.1"
                     /translation="MTFYTAKGDDGTTGLLGEGRVPKYHARMEAVGALDEASAALGLA
                     RAQSAAPQTASILLEAQRDLYKLMAEVAATPENAERFRFINEARVKWLEEQANELSAV
                     VEMPKEFILPGDTLGGAALSMARAIIRRAERRVVGLFDEEEVGNPDLQRYLNRLSSLC
                     FVLELLENQNAGKQTTLAKA"
     gene            3832..4533
                     /locus_tag="PLE39_11885"
     CDS             3832..4533
                     /locus_tag="PLE39_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HNE69075.1"
                     /translation="MTGTILNIATILLGGVIGLIFGSRIPERFKGTVIAGMGLFTAAM
                     GLQMFLKSENQLVVLGALIIGALTGEWWKLEDRLQALGQTLEQRFSQDSETGAGSKFV
                     RGFMVSSLLFCIGPMAILGSIQDGLTGDYQLLAVKSMLDGFAAIAFSSTLGVGVLFSS
                     VVVFVYQGGISLLAGQLNSIVTDPMMNELTATGGVLLIGVGISNLLELKKIRVGNFLP
                     ALIAAPLIVWILGLF"
     gene            4536..5141
                     /locus_tag="PLE39_11890"
     CDS             4536..5141
                     /locus_tag="PLE39_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69076.1"
                     /translation="MTRKRLYKILGLFSAAVLLALATAFPLIRGLLSPDSKPNPEQNI
                     FEMPLCDLQPSGLCLVTFGAISSAEMAIVIQTPGGEYPAFYLRATHQGVENTYPCEAG
                     SLPNQVICVGARTPLGEPLEIKVFTNESDLLIASGKFNVSAMATTLLGSNSGFSVMGK
                     MATPTPSPSPSPSPTVQVIFTPTSAPIATMTSTPDVGYPNP"
     gene            5283..6941
                     /locus_tag="PLE39_11895"
     CDS             5283..6941
                     /locus_tag="PLE39_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69077.1"
                     /translation="MNNELPAKSIQVLTQQWGLILALLTLIGWGSLLLYAHLKRNTGF
                     QFTDLELAALSLGGWPLPALLISFPVLFLSAFLPAGVLIGITAVILLTSGGIALRAIW
                     KGNSPWALVPVSIFLVLAFLRLGLVADVIMPMYFDSAEHYRIIRELLDSAWPTASYYH
                     LGYHVIIAAFVRISAADPASVMLIFGQLILAALPLPIFFLLYRATFSKSAALLGVIFA
                     AFGWFMPAHAVNWGKYPALLSLLLLQFTLGILLIKKNWMPAVAALAATVIHTRSVILL
                     ALFGTAWILANLWEGQPHPRRRLILAVVATMLGMLTLIIYRNPILGPILEPYATLITL
                     LSALLAASVFKAFSHPIMVSLLIILLILTAIFIPVSSSLTLLDRPLAEMILFLPLAFI
                     GGLGGARPLKFIAPALAALIILNAWMNISFGPSKCCQLVSRDDATALDWVGRHYKKDS
                     VIAIAGVNLNINAFGRPMRGAGIDAGIWINPLTGIQTVPFPNSTNFASPEAHDQLCSN
                     GITHIYVGARPQSFAPDFAELVPEWYETTFSLPKAHIVKVLNCK"
     gene            complement(7016..7774)
                     /locus_tag="PLE39_11900"
     CDS             complement(7016..7774)
                     /locus_tag="PLE39_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNE69078.1"
                     /translation="MNNKILIENLSLQYSDGTESLRGVNMEIRQNAVTVLFGPAGGGK
                     STLLRCLNRLNDLTEVRASSGQILIDGENILDPKVDVVALRRKVGMVFARPVPLPMSI
                     RENVVYGLEMAGEKRKSRLDEAVERSLKLAAIWDEVKDRLNDPANALSGGQQQRVCLA
                     RVLALQPEVVLLDEPTSGLDPISTGKVEAALQELKKEYTIILVPHSVQQAARTADHAA
                     FFLQGELIEYDEGKKMFTTPKQKKTEDYIMGRFG"
     gene            complement(7802..8665)
                     /gene="pstA"
                     /locus_tag="PLE39_11905"
     CDS             complement(7802..8665)
                     /gene="pstA"
                     /locus_tag="PLE39_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNE69079.1"
                     /translation="MMKLLARNRHTVQKLGFGLITLMAVVTVIPIVGTIVFILFKGGS
                     AISLEFLSGFPRDGMREGGILPAIIGTLYLTVGTAVFSVPLGIAAAIYLAEYAKDTAL
                     TRMIRLAIINLAGIPSVVYGLFGLGLFVLFLKFGTSILAASLTLSIMTLPVIISTAEE
                     SLRSVPQAFRTVSISLGATRWQTIRRIVLKEALPGILTGVILGLERAAGETAPILFTG
                     AAFFLPRLPGSPFDATMALPYHLFVISTQVPEMPIQIQYGTALVLLVFVLTMNVIATV
                     IRSRARAKRQW"
     gene            complement(8741..9637)
                     /gene="pstC"
                     /locus_tag="PLE39_11910"
     CDS             complement(8741..9637)
                     /gene="pstC"
                     /locus_tag="PLE39_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNE69080.1"
                     /translation="MEKSLSLREFIITRLIQASGYSAILFVALIFFFLLREGLPTLSE
                     VESSALLNVRWYPIENYFGILPLISGSLIVTLGAALIAVPFGIGTAVYISEIAPRWVR
                     EILKPLVELLGGLPSVVLGFLGILIVSPFLRVFLDLPTGLTAFTGSLLLGGIAVPTVV
                     SVAEDALDAVPRAYREGAWALGATKWQTIWRVTLPAARSGVLTAIMLGVGRAIGETMT
                     VMMVTGNAPVLASSLGSLFSPVRTMTATIAAEMGEVANGSTHYHVLFFIGMVLFLISL
                     VVNVIASAVVFRAKKRAERILS"
     gene            complement(9646..10521)
                     /locus_tag="PLE39_11915"
     CDS             complement(9646..10521)
                     /locus_tag="PLE39_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE69081.1"
                     /translation="MKRAAFFFLLVSFLLVSCGGTSSSNAVSDTAAQYIENKGSDTIV
                     NLALAWAEKYQADHVDVRISVTGGGSGTGIAALINNTVGIANASRKIKDEEIAEAQAK
                     GVTPVEHIIARDAIAVIVNPENPVNELTLQQISDIYSGKITNWSEVGGDDRPIVKLSR
                     ETNSGTHVYFLETVLRLGNKEDKTLFSTDTLLLPSSEGIISEVRDNPNAIGYDGLGYV
                     PDDLKMIAIAKEAGAPYVLPSIETVNDKSYAIARDLYMYTNGEPSGVIKEYLEWILSA
                     EAQQIVADLGFVPVK"
     gene            10668..11429
                     /gene="pstB"
                     /locus_tag="PLE39_11920"
     CDS             10668..11429
                     /gene="pstB"
                     /locus_tag="PLE39_11920"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNE69082.1"
                     /translation="MDATQPAFTIQNMDFYYSGAKKTLSNINLEIQPQKVTALIGPSG
                     CGKSTFLRCLNRMNDTIPGTHVDGKILLKGEDIYAPAMDVVTLRQRVGMVFQKPNPFP
                     QSIFDNVAFGPRVMGMDVNLNEVVEKSLTAAALWDEVKDNLKSDAMSLSLGQQQRLCI
                     ARVIAVQPEVILMDESTSALDPIATLRVEELIADLKKDYTIVIVTHNMQQAARVSDHT
                     GFFWLGELVEFAPTNQMFTVPKEELTEAYITGRMG"
     gene            complement(11496..12188)
                     /gene="phoU"
                     /locus_tag="PLE39_11925"
     CDS             complement(11496..12188)
                     /gene="phoU"
                     /locus_tag="PLE39_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNE69083.1"
                     /translation="MIRKTFESEIQLVKDEVLLLGSMVEHAIIGSVEALKKRDIKASE
                     KLIAEDKEINKKRFAIENQLMILIATQQPMAHDLRLLASTMEIISELERMGDYAKGIA
                     NINIRMGEETLLKPLIDIPRMAQKGADMLHRALTAFVNEDVQAANAIPVEDDEVDALY
                     NQIYRELMMFVIQDPKTIERANWLLWVAHNLERVADRVTNICERTVFIATGEFGEIKS
                     SDDEFWKGQKGS"
     gene            complement(12227..12682)
                     /locus_tag="PLE39_11930"
     CDS             complement(12227..12682)
                     /locus_tag="PLE39_11930"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNE69084.1"
                     /translation="MNKPKVIFLCTGNSARSQMAEGLLRALAGEHFEVFSAGTEPKGR
                     ILPEVQAVMREVGIHMEGQFSKSVMEYLGRAVFAHVVTVCADAEENCPAVFLNMGTHE
                     HWPFDDPAKFAGDDEQRITHTRRVRDQIEERLRLWLTGQGITPADYRHT"
     gene            complement(12715..13485)
                     /gene="pstB"
                     /locus_tag="PLE39_11935"
     CDS             complement(12715..13485)
                     /gene="pstB"
                     /locus_tag="PLE39_11935"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNE69085.1"
                     /translation="MEKNYAIESNKLSIHYGNFTAVRDVSIKIEPQKITAIIGPSGCG
                     KSTLLRSLNRMNDFVQGCHVTGELLLQGQNLYGKNIDPVEVRRKIGMVFQKPNPFPKS
                     IYENIAWGARINNFKGKMDDLVEQSLRSAALWDEVKDKLQQSAYALSGGQQQRLCIAR
                     TIAIQPEIILMDEPASALDPISTLRIEELMQELKKNYTIIIVTHNMQQAARASDFTAM
                     MMIDEQRAGGVIEFDETKKIFTRPQDKRTEDYVTGRFG"
     gene            complement(13510..14745)
                     /gene="pstA"
                     /locus_tag="PLE39_11940"
     CDS             complement(13510..14745)
                     /gene="pstA"
                     /locus_tag="PLE39_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNE69086.1"
                     /translation="MNMNQHYPQDREFRRMLDARYRVATLWQALFLSALLIAIVSLMA
                     LIYNVVDGAFGYIAYEYKKDPAQFTSTPIANLTQEELAAILKENLSSGAYKKLNNEQP
                     VAERSQADLFNLFMERLVQLDTKATWSMTDSLMNGEDIRAEVAEKYPKAQLEFRSWLT
                     PQFLVTPMSSKAEFAGVRTAVLGSLWLVGIAILFALPIGTGAAIYLQEYAPKNWFTAI
                     IQTNINNLAGVPSIVYGMLGLAIFVRVLEPLTSGALFGVTDSNGRTILSAGLTMGVLV
                     LPLIIINAQEAIKAVPDSLRQAAYGVGATRWQTVWYHVLPNSLPGILTGSILALSRAL
                     GETAPLIVVGASTFISLDPSGPFSKFTALPIQIYQWTTRAQSEFHAIAAGAIIVLLVL
                     LLSLNATAVLLRNRFQRRW"
     gene            complement(14750..15691)
                     /gene="pstC"
                     /locus_tag="PLE39_11945"
     CDS             complement(14750..15691)
                     /gene="pstC"
                     /locus_tag="PLE39_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNE69087.1"
                     /translation="MKQENMTAENKNFRPLNLQKKLRPAESAIQVLLFLAGVLSIFVT
                     IAIVYELGKEAMLFFTDPDVTLAKFLGTTKWQPGIGQYGIWALVSATLVTSFIAISIS
                     LPLGLAAAIYLSEYASFKSRSILKPILEVLAGIPTVVYGYFALLFVTPVLKSMIGDQL
                     QVYNMLSAGIVMGIMILPLISSMSEDALSAVPRSLREAAYAMGATKVETSLQVVVPAA
                     LSGIGAALIVGISRAVGETMIVAVASGSGPNFSFNPLDAAETMTGHIARISGGDLSYN
                     SIDYTSLFAIAMMLFLLTLVLNLISQWVVSRFRERYE"
     gene            complement(15808..16881)
                     /locus_tag="PLE39_11950"
     CDS             complement(15808..16881)
                     /locus_tag="PLE39_11950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HNE69088.1"
                     /translation="MTKFVRSFVFVFAALSFVLAACGAPAAPATEAPVVEPTEAATEA
                     PTAEPTEAPTEDPMAMYAPDAVEGDIITAGSSTVFPLSERMSELFQQEGYTGNITVDS
                     IGSGAGFERFCKTGETDIANASRPIKDSEVEACAAIGREPVEFRVGTDALAVVVSAEN
                     DFVESLTKEQLGKIYKGEFKTWDQVDAGYPAEAILVYSPGADSGTFDYFVEAVVAPLT
                     PNAEGKADVKLGEEAVLGLEGAQFSEDDNVLVQGVEGSPYAIGYFGFAYYSENSGALK
                     AINIEGVAPNQEDVDNGTYPLARPLFIYSDAKIMAEKPQVAAFIYFYLNNVNDNILDV
                     GYFPAPADALQVSMDAWMTATGN"
     gene            complement(17059..17895)
                     /gene="surE"
                     /locus_tag="PLE39_11955"
     CDS             complement(17059..17895)
                     /gene="surE"
                     /locus_tag="PLE39_11955"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNE69089.1"
                     /translation="MTKNILVTNDDGVLAPGLLALAQEMRKLGAVTILAPDRNWSGGG
                     HVKTLDRALRVREFHLSDGTQAFGSDGAPSDCVALATLGYFKEKFDLVVSGINVGANL
                     GHDVTYSGTVTAAMEAVIAGLPGVAVSLEIPEGHVGPIDFGPSALAAGMAVRNVLQHG
                     LPADTLLNVNVPFLKQEEIQGFRMTRQGLRVYHSRLDERVDPRGRPYYWIGGEAPTGV
                     PESGTDVGALSDGFVSVTPLQLDLTAYRAVSEINTWNWHDPILSEVSLLGPSSVVAAE
                     NQ"
     gene            complement(17910..18059)
                     /locus_tag="PLE39_11960"
     CDS             complement(17910..18059)
                     /locus_tag="PLE39_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69090.1"
                     /translation="MLIFCIALYLVVIVPLLALLWASLVVAKWDDKDRGYEILDEQEF
                     LELAL"
     gene            complement(18073..18780)
                     /locus_tag="PLE39_11965"
     CDS             complement(18073..18780)
                     /locus_tag="PLE39_11965"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNE69091.1"
                     /translation="MGLADLHLHTIYSYDGTAPLPAVLTRAKQLGLDVIAITDHDEIA
                     GALKALEIAPLYGVEVIPGVEITTAEGDLLALFIKEKVAAGLSLVETVLKVRELGGVC
                     IAPHPMAGGMGMKSLNAMSILKALRNRKVAETLIGIETYNGTSIDRVSNHSASLLSNR
                     LKLARTGSSDAHILETIGFGATEFEGKTAADLLAALKNRTTKVRKQNEWGMFKILGSW
                     ALNYLGSLFARRAITTN"
BASE COUNT         4707 a         5155 c         4931 g         4350 t
ORIGIN      
        1 gccggtgatc tgaggattcc aatcgccaat accagagtat ggggttgaag cgggaagacc
       61 ataagcatca gaaccaacgg cccaatactc aaagatattg caggcaaccc agtcacttac
      121 aatggcgggg tcactggggg gaacagcacc aaagccattg atgctgtgac cgtccacata
      181 ggcggaatgc atgaccacat tggcggtttc cacattcgca ccatcagcac aggaagcggt
      241 atcatcaacg gtcattacga attcccaggt gtcaccatcg gcggttgtac tggtgaaatc
      301 gtaagtgaaa ctaccggagg cagccacacc gccattcacg gtcgccacgt tcaaacattc
      361 accactgctg gcataagtgt tcacaccagc attgcgggca taaatatcca cgtagtaagt
      421 agtaccagca ggaagtgcgc ccagcgtcca ttcgaaggta gtggaagccc acggagctgc
      481 caaagcttcg gacaggaatt ccgaggtatt ggtcagggtg atatcggcac ccgccacagc
      541 ggcagcggag gcgacgctga actgcgccaa cacgaacaca gccagcagca atacggtaaa
      601 tactttcttg ctcattaata cattctcctt tgaaaagttg atctctgcca tccagtacta
      661 gattcaggcg atggctacct gaatggcgcc aattttacag ggaaacaaaa tctcgtcaac
      721 cgctgtaaat aggacgccgg tcagggcatg accatcggtc aaacaaaaaa ggagcggacc
      781 cggtgacagg tccgctccag agtttcgatt tactctattg cgggaaaatt agaaggcacc
      841 gaaatacatc agaattctgt agagggtctt ggcgaattca ccacgggtca tggcctgatc
      901 cggggagaag gtggtggcgg aggtgccatc cacaatgccc cagctgtagg cttccaccac
      961 aggttcggca tagtacgcac cgagggacac atcgctccag gccgcgtcac ggtaacgagg
     1021 ggcagcatca tcgaaggcaa gcaaaccctg tccaacaacc atatgagagc ccggctggag
     1081 attgcccaaa ccaagttcgt agatgttgtt gaagatggtg acgatctggc cgcgggtgag
     1141 caggttgttc gggtcgaaca agccaccgcc cacaccgttc ataaagccgt catcacaagc
     1201 ctgccagacc cagccagcga accagtcggt ggcggccacg tcggtgaagt cacaaccggc
     1261 gggggcagag gtcggcaggg tggtggcatc gtagccgttg gagatctcga tcagggcaga
     1321 ggcttcggcg cgggtcaggg tgttgtcggg gccgaaggtg ccatcggtga agccgctgat
     1381 caggccgagg tcggccaggt agcggatata cacttcatgt tcgtgaccag ccacatcgct
     1441 gaaatccgcg caatcttcaa gttcgtcagc cgaaacgttg ctgttgcgga agtagtaagg
     1501 gccagcattc agattaccag ctgcatcttc ggggtcaatc gagaactgcc acaagccgct
     1561 tgcggtggca ggcagggtaa tcttccagga gagcagctca tcttccgcac caattggggc
     1621 aggagcaaag gtgccggtgg tcttggcatt ccatgaaccg aatccagagt acggatcaga
     1681 ggggttcgcc aaaccatagg tatcggaagc cagcgcccag tattcaaagg tgttgcaggc
     1741 cacatcgcca gcagcagtga aatcggggtg aggaatgacc agaatacctt ccaattcagt
     1801 gccatccaca taggaggagg cagagaagtt cgcatccaca ttggcattgg caggcagggt
     1861 ggtgcaggaa gccgcatcgt caaacgcgat caggaactcc caatattcgc catcggcagg
     1921 gttgccatac acaccaccac cagtgaacac ataatcaaat gtgccgctac cgttgctgga
     1981 accattgatg aaggctacat tgttacattc agccacatca taggtgttgc ctgaccccat
     2041 gagacgggca tagatatcca cataggtgcc agcgggcaga ccagtggcgg tccattccag
     2101 ggtaacagtg gagcctgcac ctgcgggcat aaagtccggg ccggtgaggg tgccatctgc
     2161 ggtgacttgt gcagcggagg cgatgctgaa ctgtgccaac acgaacacag ccagcagcaa
     2221 tacggtaaat actttcttgc tcattgcttt tctccttgta taagatttga tttctgccat
     2281 ccattactag attcagatga tggcgacctg aattcactta ttttacaggg aaacaaaatc
     2341 tcgtcaaccg ctgtaaatag gacgacggtc agggcatgac cattggttta gaaaataaag
     2401 agtaggcttt ttacatatcc acaaatttgg agaacatttc aggggtgagc aatttttcca
     2461 atagatcgaa ttcgtagatg gtacgtttga ccgcagcagc acgagcgtag gcaaattttg
     2521 ccccctgaac aggcgaatca agattcatct tcgcatgaga atccgctggg agcagggaca
     2581 gtgataattt gcggagaaaa ggtacaaagt acagccagtc catgggcatg tagagtttgc
     2641 cggagtgcag gcttgggctt tcgatcagat ccttttgaac gggatcaatt tcgggcgggg
     2701 tgggaggcgt gccgccgggg tgtgcatcaa gccacatggt gacgtagttg gtatgggagt
     2761 agaaatcctg tacggtgtgg gtcaatctgc caaaagcgat ccaggcagag aggacaccag
     2821 gcgaaagcag ggatgcgatg acaaatccgc gctgttcgat gacgtaccga tgaccttcgt
     2881 caatggcgtt gttatcataa tggaattcat catggccgat ctgaccgctg agggcatctt
     2941 gtttgcaatt ggccgcgatg atgacctcaa gcgcacgcgg actgacacgg ccctcaaggg
     3001 cttcgcgggt catttcctga tgaatgggga taagcatttc aaaattatac ccagtctggg
     3061 ctggagaatc tgtgtggagc ttcaaactgg ttataatgcc tgcatgactt tttatactgc
     3121 aaagggtgac gacggcacaa caggcctgct gggcgagggc cgtgtcccca aatatcatgc
     3181 ccgcatggaa gcggtcggcg cattggatga agcctcggct gcgctggggc tggcacgtgc
     3241 acaatccgcg gcaccgcaaa cggcttcgat tttgcttgaa gcacagcgcg atctgtacaa
     3301 actgatggct gaggtggcgg ccacaccgga aaacgcagag cgtttccgct tcatcaatga
     3361 agcccgcgtg aaatggcttg aagaacaggc caatgaatta agtgcggtcg ttgaaatgcc
     3421 aaaagagttc attttgccag gtgacacgct tggcggggcg gctctttcaa tggcgcgtgc
     3481 catcattcgc cgtgctgaac gccgtgtggt ggggctgttc gatgaagagg aagtaggcaa
     3541 ccccgatctg cagcgttatc tcaatcggct ttcatcactg tgttttgtgc ttgaattgct
     3601 tgaaaaccaa aacgcgggca aacaaactac actggcaaaa gcataaaaac aacagagtgc
     3661 cttggataaa gaatggcacc ctgtattact ctacatttaa ttaaactcac cttacgcagc
     3721 gctgtgaaaa ctgatgaagg cattgaccgc agacggcaga ccacgatcaa agtttagaca
     3781 aacctcgcgc cgtatcaact caagtgcgca aagtgagcac acaaggaaaa catgacaggt
     3841 acgatactca atattgcaac cattcttctg ggcggggtta ttggtttgat cttcggttca
     3901 cgtattccgg agcggttcaa aggcaccgtc attgccggca tgggattgtt caccgcggca
     3961 atgggactgc agatgttctt gaaaagtgaa aaccagttgg tggtgctcgg cgcactcatc
     4021 atcggcgcat tgaccggcga atggtggaaa cttgaagaca ggctgcaagc cctggggcaa
     4081 accctcgaac agcgtttctc gcaagattca gaaacaggtg cgggctccaa attcgtgcgt
     4141 ggattcatgg tatcctcgct tttattttgc atcggcccga tggccatcct tggctccatt
     4201 caggatggtt tgacaggcga ttaccaactg ctggctgtaa aatccatgct ggatggtttc
     4261 gccgcaatcg cattttcatc cacgttggga gtgggtgtat tattctcatc ggtcgtcgtg
     4321 ttcgtgtacc agggcggaat cagtctgctc gccgggcagc ttaattccat cgtcaccgac
     4381 ccgatgatga atgaattgac cgccaccggc ggggtgcttt tgatcggggt gggcatcagc
     4441 aacctgctgg aactcaaaaa gattcgcgtt gggaatttcc tgcctgcatt aattgcggct
     4501 ccattgatcg tttggatcct gggtttattt tagaaatgac acgaaaaaga ctctacaaaa
     4561 ttttaggact attcagcgct gcggttctgc ttgcgctggc aactgctttt ccgcttatca
     4621 gagggctgct ctcccccgat tcaaaaccca accctgagca aaacatcttc gaaatgccct
     4681 tgtgtgatct gcagcccagc gggctatgcc ttgtcacttt tggcgccatc tcctcagcag
     4741 aaatggccat tgtcatccaa acacccggtg gagaataccc cgccttttac ctgcgtgcca
     4801 cacatcaagg tgtggaaaac acctatccgt gcgaggcggg gtccctgccc aatcaggtca
     4861 tctgtgtggg tgcgcgcacc cctcttggag aaccgcttga gatcaaggta tttaccaacg
     4921 aaagcgatct gctgatcgcc agtggaaaat tcaatgtctc tgcaatggcg accacccttt
     4981 tagggagcaa cagcggattc agtgtcatgg gcaaaatggc cacccccacc ccatctccct
     5041 cgccttcccc atcgcccacc gtgcaagtca tcttcacacc cacttccgct cccatagcca
     5101 ctatgacttc cacaccggac gttggttacc ccaacccata aatgacaaat ttgaactaca
     5161 aagatcgcaa agaaaaaagt taaaacgcag cgatcttcac agtaaaacgg gcgaatgtgc
     5221 ggtagaagaa cttcaacccc tgttttcccc cagtcattac ggttcaaaaa agctaaattc
     5281 ttatgaacaa cgaactgcca gccaaaagca ttcaagtgct cactcaacaa tgggggctaa
     5341 tccttgcatt gctgaccctc ataggctggg gctccctgtt gctctacgct catttaaaac
     5401 ggaacacagg cttccagttc accgatcttg agttagccgc cctatccttg ggcggctggc
     5461 ccctgcctgc cttgttgatt tcctttccag tgctgtttct aagcgcgttt cttcctgctg
     5521 gcgttctcat cggcattacg gcggtcatcc ttcttaccag cggaggaatt gcccttcgcg
     5581 ccatttggaa ggggaattcg ccctgggcgt tggtccctgt ttccattttt ctcgtgctgg
     5641 cttttctgcg gctgggtctt gtggccgatg tgatcatgcc aatgtatttc gactctgccg
     5701 aacattaccg catcattcgt gaattactcg attccgcctg gcccaccgct tcttattatc
     5761 accttggcta tcacgtcatc atcgcagcct ttgtgcgcat cagcgccgct gaccctgcca
     5821 gtgtcatgct catcttcggg caattgattc ttgcagcact gcccctgccg atcttctttt
     5881 tgctgtaccg cgccaccttc tcgaaatcgg cggcactcct cggtgtgatc ttcgccgctt
     5941 tcggctggtt catgccggca catgcggtca actgggggaa gtatcccgcc ctgcttagcc
     6001 tgctcttgct tcaattcacg ttgggaatcc ttctcatcaa aaaaaattgg atgcccgctg
     6061 tggcggcact tgccgccacg gtcatccaca cccgttcggt gattctgctt gccctttttg
     6121 gcacggcatg gattcttgcc aacctgtggg aaggtcaacc ccacccaagg cgccgcctca
     6181 ttttggcggt ggtcgccacc atgctgggaa tgctgactct gatcatttac cggaacccga
     6241 ttctcggtcc gattttggag ccgtatgcga cgctcatcac gcttctttcc gcgctgttgg
     6301 ctgcttccgt tttcaaggcc ttctctcacc cgatcatggt ctctctgctc atcatcctgc
     6361 ttatccttac agccattttt atccccgtct cttcatcgtt gaccctgctt gacaggccat
     6421 tggccgaaat gatcctcttt ctaccgcttg ccttcattgg cgggttgggc ggtgcgcgtc
     6481 cgctgaaatt tattgcgcct gcgctggctg cattgatcat cctgaatgca tggatgaaca
     6541 tcagcttcgg accttcgaaa tgctgtcaac tggtaagccg tgacgatgca accgcgcttg
     6601 attgggtcgg caggcattac aaaaaggatt cggtcatcgc cattgcgggg gtcaacctga
     6661 atattaacgc ttttggcaga cccatgcgcg gtgcaggcat tgatgcgggc atttggatta
     6721 acccgctgac agggatccaa accgtcccct tccccaactc caccaacttt gcttcaccag
     6781 aagctcatga ccagctttgc agcaacggga tcacccacat ttatgtcggc gcaaggccgc
     6841 agagttttgc ccccgatttt gccgagttgg ttccagaatg gtacgaaacc accttctcgc
     6901 ttccgaaagc acatattgtc aaagtgctta actgtaaata acccaacttg ccgcctttag
     6961 cagaggaatt caaaaaagac cgtcgacggt tgtcgacggt cttttacttc tgcgcttatc
     7021 caaaacgccc catgatgtaa tcttcggttt tcttttgctt cggcgtggtg aacatctttt
     7081 tgccttcgtc atattcgatc aactcaccct gcaaaaagaa agccgcatgg tcggcggtcc
     7141 gcgccgcctg ctgaacggaa tgcggcacaa ggatgatcgt gtattccttt ttcaattcct
     7201 gcaaggcggc ttccaccttg cccgtggaga tcgggtcgag acccgaagtg ggttcatcga
     7261 gcaaaacgac ttcaggttgc agggccagca cacgcgcaag gcagacacgt tgctgctgcc
     7321 cgcctgaaag cgcattcgca gggtcattca atcggtcttt cacttcatcc cagatcgcgg
     7381 caagtttcaa actgcgctcc acagcctcat ccagcctgga ttttcgcttt tcacccgcca
     7441 tttccaatcc ataaaccaca ttctcgcgga tgctcattgg cagtggtacg ggacgggcaa
     7501 acaccatccc caccttgcgg cgcagggcca caacatcaac cttcgggtca aggatgtttt
     7561 caccatcgat caaaatctga cctgaactgg cgcgcacttc ggtcaggtca tttaagcggt
     7621 tgagacagcg cagcagcgtg gattttccgc cacctgccgg gccgaacaaa acggtcaccg
     7681 cattctggcg aatttccatg ttcacaccgc gcagtgactc ggtgccgtcg gaatattgca
     7741 gggaaagatt ttcgataagg attttattgt tcatttgcga tattcaactt gcaatggatg
     7801 gttaccactg gcgtttcgca cgtgcccgtg aacggatcac cgtggcgatg acattcatgg
     7861 tcagcacaaa aaccagcagg acgagcgccg taccgtattg gatctggatc ggcatttcag
     7921 ggacctgtgt ggaaatcaca aatagatgat acggcaatgc catggtcgca tcgaatgggg
     7981 aaccgggcaa acgcggcaaa aagaaggctg cacccgtaaa aaggatcggc gccgtttcac
     8041 cggcagcccg ttccaacccc aggatgaccc cagtgagaat accgggcagg gcctccttta
     8101 acacaattcg gcggatggtc tgccaacggg tggctccaag agaaatgctc accgtgcgga
     8161 atgcctgcgg cacggaacgc agcgactctt ctgcggtgct gatgatgaca ggcagggtca
     8221 taatggaaag ggtcagggaa gcggcgagga tggatgtgcc gaatttcaga aaaagcacga
     8281 acagacccag gccaaacaag ccatacacca cggaaggaat ccctgccaga ttaatgatcg
     8341 cgagacggat catgcgagtc aacgccgtgt ctttggcata ttctgccaga taaatggccg
     8401 cagcaatgcc caagggcacg gagaaaacag cggtgcccac cgtcaggtac aacgtaccga
     8461 tgatcgcggg aaggattccg ccttcgcgca ttccatcgcg ggggaaaccc gaaaggaatt
     8521 cgagcgaaat ggccgaaccg cccttgaaaa gaatgaaaac gatcgtaccg acaatgggaa
     8581 tgaccgtgac gaccgccatc aaagtgatga ggccgaaacc gagcttttga accgtatgac
     8641 gattgcgtgc aagtaatttc atcataattg aaaacagggc ttctttcttc ttctctcttt
     8701 cctttacgcc attcgataaa aaagaaaggg agcaaaaact ctacgaaagg attctctccg
     8761 ctcttttctt ggcacggaag accaccgccg aagcgatgac attgacgacc agtgagatca
     8821 ggaagagcac catgccaatg aagaacaaca catgataatg tgtgctgccg ttcgccacct
     8881 cacccatttc agcggcaatg gtcgcggtca tggtgcggac cggggaaaag aggctgccca
     8941 aactggaagc cagcacaggc gcattccctg tcaccatcat cacggtcatg gtttcgccga
     9001 tggcgcgccc aaccccgagc atgatggcag tgagtacacc cgaacgggcg gcaggcaggg
     9061 tcacccgcca gatggtctgc catttggtgg cacccaaagc ccaggcacct tcacgatacg
     9121 cacgcggaac tgcgtccagc gcatcttcgg ctacagagac caccgtcggc acggcgatgc
     9181 cacccaacag cagggatcct gtaaaagcgg tcagacccgt tggcaggtcc agaaagacac
     9241 gcagaaatgg agagacgatc aaaattccaa gaaagcccaa caccacagaa ggaagtccgc
     9301 ccagcaactc caccaagggt ttgagaattt cacgcaccca gcgcggagcg atctcggaaa
     9361 tatacacggc ggtaccaatt ccaaacggaa cggcgatcaa tgccgcgccc agtgtaacga
     9421 tgagcgaacc gctgatcaac ggaagaatgc cgaaataatt ttcgatcggg taccagcgca
     9481 cattgagcag ggctgaagat tccacttcgc tcaaggtggg caatccttcg cgcaaaagga
     9541 agaagaagat caacgccacg aacagaatgg cagaataacc cgaagcctgg atcaggcggg
     9601 tgatgatgaa ctcacgaagg gacagggatt tttccatggc ggtctctatt tcacaggcac
     9661 aaacccaaga tcggccacaa tctgctgagc ctcggcggaa aggatccact caagatactc
     9721 tttgatgacg ccgctgggtt caccattggt atacatgtaa agatcgcgcg cgatcgcata
     9781 acttttgtcg ttcacagttt cgatcgaagg cagcacatag ggtgcgcccg cttcttttgc
     9841 aatggcgatc attttcagat catcgggcac ataccccaac ccatcatagc cgatggcatt
     9901 cgggttatca cgcacttcag agatgatgcc ctccgaagac ggcagcagca acgtatctgt
     9961 ggaaaacagg gtcttatctt ccttgtttcc caaacgcagc acggtctcca aaaaatatac
    10021 atgcgtaccc gaattggttt cacgcgaaag ttttacgatc ggacgatcat caccgcccac
    10081 ctcgctccaa ttggtgatct tgccgctata aatatcggat atctgttgca aggtcaactc
    10141 gttgacaggg ttttcaggat tcacgatcac agcaatggca tcgcgcgcga tgatatgttc
    10201 aacaggcgtc acacctttcg cttgtgcctc ggcaatctcc tcatccttga tcttgcgtga
    10261 agcattggcg atccccactg tgttgttgat gagagccgca attcccgtcc ctgaaccgcc
    10321 gcccgtcacc gaaatgcgca catcaacatg gtccgcctga tatttctcag cccaagccaa
    10381 cgccagattg acgatggtat cggaaccctt gttttcaatg tattgagccg ctgtatcaga
    10441 aacagcattg gaagaagatg tccctccgca ggaaacaagc aggaaagaga caagtaaaaa
    10501 gaaaaaagca gcgcgtttca ttgggatgga ttatagtcgt tggcaggctc agaaacaagt
    10561 agacaaatcg aaacaaacgc gccgaaaact gaccctgatc caaaaaatca aatctccctt
    10621 tatcattcgc cctcatcctt catccctttc tttgataaaa tcatttcatg gacgcaaccc
    10681 aacccgcctt caccatccaa aacatggatt tttactacag cggcgccaaa aagacgctgt
    10741 cgaatatcaa ccttgagatt cagccgcaaa aagtgaccgc actcatcggc ccgtcaggat
    10801 gcggaaaatc aacgttttta cgctgcctca atcgcatgaa cgacaccatc cccggcacac
    10861 atgtggacgg gaagatcctg ctcaaaggcg aggatatcta cgcccccgcc atggatgtgg
    10921 tcaccctgcg ccagcgggtg ggcatggtct ttcaaaaacc gaatccattt ccacagtcca
    10981 ttttcgataa cgtggctttt ggtccacgcg tgatgggcat ggatgtaaac ctgaacgaag
    11041 tggtggaaaa aagcctcacc gccgccgcat tgtgggatga agtgaaagac aatttgaaat
    11101 ccgacgccat gagcctatcc ctcggccagc agcagcgttt gtgcattgcc cgcgtgatcg
    11161 ccgttcaacc tgaagtcatc ctcatggatg agtccacttc tgcgcttgac cccatcgcca
    11221 ccctgcgcgt ggaagaactg atcgccgatt tgaaaaaaga ttacaccatc gtcatcgtca
    11281 cccacaacat gcagcaggcc gcccgcgttt cagaccacac cggcttcttc tggctgggag
    11341 aattggtcga gttcgcaccg accaatcaga tgttcaccgt tccaaaagag gaactgaccg
    11401 aagcctacat cacaggcagg atgggataga aattaacaat gaaaataaga acccgccccg
    11461 agtatttcgg ggcgggtttg ttttgccaat tctttttacg aacccttctg acctttccag
    11521 aattcatcat cactggattt gatctcgcca aattcacctg tggcgataaa tacggtacgc
    11581 tcgcagatat tggtcacacg gtcggccaca cgttccagat tatgggccac ccacaacaac
    11641 caattcgcgc gctcaatggt ctttggatcc tggatcacga acatcatcaa ctcgcgatag
    11701 atctggttgt agagcgcatc cacctcatca tcttccacag ggatggcatt ggcagcctgc
    11761 acatcttcgt tgacaaaagc ggtcaacgca cgatggagca tatcggcgcc tttttgagcc
    11821 atgcgcggaa tatcgatcaa cggcttgagc agggtttcct cacccatgcg aatgttgatg
    11881 ttggcgatgc ctttggcgta atcgcccatg cgctccaact ctgaaatgat ttccatggtg
    11941 gaggccagca ggcgcaggtc atgtgccatt ggctgctggg tcgcaatgag gatcatcaac
    12001 tgattttcga tcgcaaaccg ctttttgttg atttccttgt cttcggcgat taatttctca
    12061 gaagccttga tgtcgcgctt cttcaacgct tccaccgagc cgatgatggc atgttccacc
    12121 atgctgccca gcaaaagcac ttcatctttc accagttgaa tttcactctc aaatgttttt
    12181 ctgatcataa gacctctgat ggtttttctt attttactac catcaactac gtgtgacgat
    12241 agtccgctgg ggtaatgccc tgcccggtca gccaaaggcg caggcgctcc tcaatttggt
    12301 cgcgcacgcg tcgggtatgg gtgatgcgct gttcgtcatc cccggcgaat ttggcaggat
    12361 catcaaaagg ccagtgttca tgtgtcccca tgttgaggaa gacagcagga caattctctt
    12421 cggcatcggc acacactgtg acgacgtgcg caaaaacagc ccgtccaaga tattccatga
    12481 ccgatttgga aaattgcccc tccatatgga tgcccacctc gcgcatcacc gcctggacct
    12541 cgggcaggat cctgcccttt ggctcagtgc ctgcgctgaa cacttcgaag tgctcccctg
    12601 ccagggcacg cagcaaccct tcagccatct ggctgcgtgc ggagttgcca gtgcaaagaa
    12661 aaatgacctt tggtttgttc atcgttttct ccatgatttc attgcgaggg tgtgttaccc
    12721 aaagcgtccc gtcacataat cttcggtgcg tttatcctgc ggacgggtga atatcttttt
    12781 ggtttcatca aattcgatca caccgcctgc gcgctgttca tcgatcatca tcatggcggt
    12841 gaaatcagaa gcacgtgccg cctgctgcat gttgtgggta acgatgatga tggtgtagtt
    12901 cttcttcaac tcctgcatca gttcttcgat gcggagcgtg gagatggggt cgagcgcgga
    12961 agcaggttca tccatgagaa tgatctcagg ctgaatggcg atggtgcggg caatgcataa
    13021 ccgctgctgc tgaccgccgg agagggcata tgcgctttgt tgaagtttat ccttcacctc
    13081 atcccacaag gcggcactgc gcagggattg ttctacaagg tcatccattt ttcccttgaa
    13141 gttattaatg cgtgcgcccc aggcaatgtt ttcataaatg gatttcggga acgggtttgg
    13201 cttttgaaag accatgccga tcttgcggcg cacttccacg gggtcaatat ttttaccata
    13261 gaggttttgt ccctgcagca acaattcgcc ggtgacatga cagccctgca caaaatcgtt
    13321 catgcggttc aacgaacgca gcagcgtgga ttttccacac cctgaaggcc cgatgatcgc
    13381 agtgatcttc tgcggctcga ttttaatgct tacatccctt actgcggtga aattgccgta
    13441 atgaatggag agcttgttgg attcgattgc gtaatttttt tccatgggtt tatatccaca
    13501 aattccagat taccagcgtc tctgaaagcg attacgcagc aaaaccgcgg ttgcattcag
    13561 cgacaaaaga agaacgagca ggacaatgat cgcacccgcc gcaatggcat ggaattcaga
    13621 ctgggcgcgg gtggtccatt gatagatttg aatgggcagg gccgtgaatt ttgaaaatgg
    13681 cccggatggg tcgaggctga taaaagtgga ggcccccacc acgatcaacg gtgcggtctc
    13741 gccaagcgca cgcgaaagcg caagaatgct gccggtcaga ataccgggca atgaattcgg
    13801 cagcacatgg taccaaacgg tctgccagcg ggtggcaccg acgccgtacg ccgcctgcct
    13861 gagcgaatcc ggcactgcct tgatcgcctc ctgtgcgttg atgatgatca acggaagaac
    13921 aagcacaccc atcgtcagcc ccgcggaaag gatcgtgcga ccattcgagt ctgtgacgcc
    13981 aaacaaggcc ccactggtca aaggctccaa cacacggaca aagatggcca gccccaacat
    14041 gccatagacg atgctgggca cacccgccag gttattgatg ttggtctgaa taatggcggt
    14101 gaaccaattc ttcggcgcat attcctgcaa atagatcgcc gcgccggtgc cgatcggcag
    14161 tgcgaacagg atcgcaattc ccaccagcca caacgacccc aacacagcgg tccgcacccc
    14221 tgcgaattca gccttcgatg acatgggtgt gaccaaaaac tgcggggtga gccatgagcg
    14281 gaattcaagc tgtgccttgg ggtatttctc tgcaacctct gcacggatat cctcgccatt
    14341 catcaacgaa tcggtcatac tccaggtggc tttggtatcc aactgcacaa gacgttccat
    14401 gaacaggttg aacagatccg cttgtgaacg ctcggccaca ggctgttcat tgtttaattt
    14461 cttgtatgca cccgaagaaa gattctcttt gagaatggcg gccagttcct cctgagtcag
    14521 attcgcgatc ggggtggagg tgaattgagc gggatccttt ttatattcgt aggcaatgta
    14581 gccgaaggcg ccatccacca cgttgtaaat caacgccatc aaactgacga tcgcgatcaa
    14641 caacgccgag aggaacaggg cctgccaaag ggtcgccaca cgataacgcg catcgagcat
    14701 gcgccgaaat tcccggtctt gcgggtaatg ttgattcata ttcatgtgat tactcatatc
    14761 tttccctgaa gcggctcacc acccattgac tgatcagatt caacacaagg gtcaacagaa
    14821 acaacatcat cgcaatggca aagaggctgg tgtaatcaat gctgttatag gaaaggtcac
    14881 cgccgctgat gcgggcgata tgaccggtca tggtttcagc cgcatccagt ggattgaacg
    14941 aaaaattcgg gcctgagcct gatgccacag caacgatcat cgtctccccg acagcgcgtg
    15001 aaatgcccac gatcagcgcg gcgccgatcc ccgaaagcgc cgcaggcaca accacctgca
    15061 ggctggtctc cactttggtg gcacccattg cataagcggc ttcgcgcagt gaacgaggca
    15121 cggcgctcaa ggcatcctcg ctcatcgaag agatcagcgg caggatcatg atgcccatca
    15181 cgatccccgc cgacagcatg ttataaactt gaagctgatc gccgatcatg gatttcaata
    15241 ccggggtgac gaacaacaag gcaaaatacc cgtaaacaac cgttgggata ccggcaagca
    15301 cttcaagaat tggtttcaga atggaacgtg acttaaagga ggcatattca ctgaggtaga
    15361 tggcggcagc caacccaagc gggagggaga tggagatggc gataaaactt gttaccaggg
    15421 tggctgagac cagggcccaa ataccgtatt gtccgatacc tggctgccat tttgtggtgc
    15481 ccaggaattt tgccagcgtt acatcggggt cggtgaaaaa tagcatggct tccttgccca
    15541 attcatacac aatggcaatg gtcacaaaaa tagacaaaac tcccgcaaga aaaagcaaca
    15601 cttgaatggc gctttcagcg gggcggagtt tcttttgtaa attgagcggg cggaagtttt
    15661 tattttccgc ggtcatattt tcctgtttca tgttcgctct atggtttcca gtccagatcc
    15721 acttaacgga gcaatgtgtg gatctgtgat tacagcataa atgggctggc ctcaattcag
    15781 agaccagccc gtcggcatat ttttctttta gttcccggtg gcagtcatcc aggcgtccat
    15841 cgaaacctga agggcatcgg caggggcggg gaagtaaccc acatcgagaa tgttgtcgtt
    15901 gacgttgttc aggtaaaagt agatgaacgc agcaacctgg ggcttctcgg ccatgatctt
    15961 cgcatccgag tagatgaaga gcgggcgggc cagcggatag gttccgttat ccacatcttc
    16021 ctgattcggt gcaacacctt cgatattgat ggccttcaaa gcaccggagt tctcagagta
    16081 gtaggcgaag ccaaagtaac caattgcgta ggggctgcct tccacacctt gaacaaggac
    16141 attgtcatct tccgagaact gcgcaccctc gagaccaagg acggcttctt caccaagttt
    16201 cacatcggct ttgccttcgg cattcggggt caacggagca accacggctt ccacaaaata
    16261 atcaaacgta ccactgtctg cgccggggct gtacacaagg atggcttcag cggggtagcc
    16321 tgcatccacc tgatcccagg tcttgaactc gcctttgtag atcttgccaa gctgttcctt
    16381 ggtgagagat tcaacaaaat cattttcggc actcacaaca acggcaaggg catcggttcc
    16441 cacacggaat tcgactggtt cgcggccaat tgctgcacag gcttcaactt cgctgtcttt
    16501 gatcgggcgg gaggcattcg caatgtcggt ttcaccggtc ttgcagaaac gctcgaaacc
    16561 agcgccagaa ccgatgctat ccacggtgat gttgccggta tagccttcct gttggaagag
    16621 ctcagacatg cgttccgaaa gtgggaacac tgtggaagaa ccagcagtga tgatgtcacc
    16681 ttccacggcg tcgggggcgt acatcgccat tgggtcttca gtgggggcct cggtgggttc
    16741 agcagtgggc gcttcggtag cggcctcggt gggctcaaca acaggggctt cggtggcagg
    16801 ggcggcaggc gctccgcagg cggcaagcac aaagctcaaa gccgcaaaaa caaaaacaaa
    16861 tgaacgtacg aatttggtca tctctattct cctatgtttt atataacgat gaggatgata
    16921 acaagaggct cttaaggtca atggaagaat gggttaacgt cttgttaagg cttgggacga
    16981 gggcgttaaa caaaatgtcc accatttctg gtggacaccg tctccgatct ggagacacga
    17041 aggaggagaa gaacgctgct attgattctc tgccgcaaca acagaagaag gtcccagcaa
    17101 cgagacttca gaaagaatgg ggtcgtgcca attccatgtg ttgatctctg agacggcgcg
    17161 ataggcggtg agatcaagct gcaacggggt gaccgaaaca aatccatcgg ataaagcgcc
    17221 gacatcggtt cccgactcag ggacacctgt tggggcttcc ccgccgatcc agtaataggg
    17281 acggccgcgc gggtccacac gttcatcgag tcggctgtga tacacgcgca agccttgtcg
    17341 tgtcatacga aaaccttgaa tctcttcctg tttgaggaac ggtacattga cgttcaacaa
    17401 tgtgtcagcg ggcaacccat gctgcaggac gttccgaaca gccatccctg cggcaagagc
    17461 ggaaggtcca aaatcgatcg gaccgacatg cccttcgggt atttccaaag aaacagcaac
    17521 accgggcagc cctgcgatca cggcttccat tgcagcggtt acggttccgg agtaggtcac
    17581 atcgtggccc aggttcgcac cgacattaat gcccgaaaca accaggtcaa atttctcctt
    17641 gaaatatccc agggttgcca gcgccacgca atcggaaggg gcgccgtcac taccaaaagc
    17701 ctgcgtacca tcgctgagat gaaactcgcg cacacgcaag gcacggtcca gtgtttttac
    17761 atgaccgccg ccagaccagt ttcggtcggg ggcaaggatc gtcactgcgc caagtttacg
    17821 catttcctgt gccagagcca gaagtccggg cgcaagcaca ccatcatcat ttgtaaccag
    17881 tatgttcttt gtcattctat ccattcttct taaagagcca attccaaaaa ttcctgctca
    17941 tccaaaatct catagccacg gtctttatcg tcccatttgg caacgaccaa tgaagcccat
    18001 agcaatgcaa gcaggggtac gatcacaacc aaatacagcg caatgcaaaa aatcaacatc
    18061 tcaacctacc cttcaatttg tcgttatcgc tcgtcgtgca aagaggctgc cgagatagtt
    18121 caatgcccaa ctgcccagta ttttgaacat gccccactca ttctgcttgc ggacctttgt
    18181 ggttcggttc ttcaaagcgg ccagcaggtc ggcggctgtt ttgccttcga attcagttgc
    18241 tccgaaaccg atggtttcga gtatatgagc gtcgctgctg cctgtccgcg cgagtttgag
    18301 gcggttcgaa agcaggctgg ccgagtgatt gctaaccctg tcaatggagg tgccgttgta
    18361 ggtttcaatt ccgatcaggg tttcagccac ctttcggttg cgcagggctt tcaaaatgga
    18421 catggcgttc aaactcttca tccccattcc gcccgccatg gggtgcggcg caatgcatac
    18481 cccgccgagt tcacgcacct tcaaaaccgt ttccacaagt gaaaggccag cggctacttt
    18541 ttctttgatg aagagggcca gcaagtcgcc ttcagccgtg gtgatttcga cgcccgggat
    18601 cacttccacc ccgtacaggg gtgcaatttc cagggctttc aaggcacctg caatctcgtc
    18661 atggtcggta atggcgatga catccaaccc cagttgtttc gcacgggtca agacagcggg
    18721 caggggtgcg gtgccgtcat agctatagat cgtgtgtaaa tgcaaatctg cgagtcccat
    18781 tcaatttctc ctaaaaatgg acattgaagt ccatgtcaca tgtcttttca tttccacagt
    18841 gtatctgtgg accattaagc tgctgtgaag atcgttttaa gaaattgtat gggttgtgtt
    18901 aacaaaagcc gcccgccatc atgcatgacg gcgggcggcc tgcctcgtgg aggcaatgtg
    18961 aggagaaaac caatgtcagt tacggctgcg tttcttcagg tagtcgcggc gcttgcgggt
    19021 cgcatccacc ccaaagacga ggaccatgga tacaaaaata acagaagata aaatggtttc
    19081 catatgtttc tgttcctata ctgaaatcat acttcaaagc ctttattttc gacttaagag
    19141 cca
//
LOCUS       DAOQJC010000074        18975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1336_length_18975_cov_9.667759, whole genome shotgun sequence.
ACCESSION   DAOQJC010000074 DAOQJC010000000
VERSION     DAOQJC010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18975
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1336_length_18975_cov_9.667759"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..3749
                     /locus_tag="PLE39_11970"
     CDS             174..3749
                     /locus_tag="PLE39_11970"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE69092.1"
                     /translation="MDNKKTYPLHLDDESRQHTRWGTILLSTILVLVYAYVLTQIFIG
                     EKTTQSYIAMAVLLLVSTSTVMGLVLSIRQRTELGLKLTYYSLIALCVAAVVLFQGRT
                     TSITFSILSISTVGFIWLFPQHTRREYATIMIVSLVAIWVTELANPAWRLSMNMAQVG
                     PAGAGIFVILFAIYIFRESQNVISSNLRLKITIWTGLILTGISIILIVYSQIIARQAA
                     VDAAEKEALSFAESQARSVRADVEIPLDTARALAHALTSTKEPSNDEVLSRTQVNAML
                     RQVLLENPNFLGTYTLWEPNAFDGLDQTFKGTAAHDNTGRFIPYWVRGDDGSLTVTAL
                     LDYETPGLGDWYLIPRQTQKEVTIAPLIYPIQGVDTVMASFVVPIIYADKFYGIAGVD
                     APIAFVQNLVDQVDLYNGKAGAVLMTSDGTLISVRNQPELVNQPAAEIFPDFLELQSR
                     IQAGESFISLSPDGDSLRAFAAVDLGQTGDHWSFVLLVPFSEIIAPANTAAIRQGVIG
                     LVLIATALAILWFLSGQIVRPVRELTTVANAISQGNLNVTARVQAADETGLLASTINL
                     MITQLRESFVTLEERVAERTQNLELASQVGRSVSQLRKLDVMLKDAAEIIRSRFDLYY
                     VQVYLTNPSKTYLNLQAGTGHVGEELLARNHRLPINTGSINGRAAVEKRSVIIADTST
                     STTFRPNPLLPNTRSEMAVPLILGETVVGVLDMQSETAGALNHEMLSAFEALAGQLAI
                     AIQNANLLAEAEQARAEVEAQAQRLTRTNWASYLDAIHKPEESGYVFEQNKVMPMVEA
                     EDQTVNDDALTAPIAITGETIGNLVVELEGQSPLTRPNELINIVARQVAQQIENLRLL
                     ENAERYRVEAEEASRRLIREGWKTYKEGAKNNLSYLYDLKEVRPLNGDSTSEPALSIP
                     LKVRDELIGELAIQGMNLEDGDSASLAAAVADRLSSHIETLRQYDQAQSALEQSEKLF
                     DASRRLTQATSLQDLVAATVESLDIPVVNRAVLGIFNYGADDKLESMSIEANWWNGTG
                     HNVTAIGVRYPVAVFRTVSLFASPVPLFFADAFNDERADAATLQLAKQLNLRAVAVLP
                     LYIGTRQVGVLMLEAEQPYHFSQDEIRLFSSLAPQIATVLENRRQFDRAQQQAEREST
                     LNLISQKIQSATTVDAVLQIAARELGHALGAPMTIAQLSMKDKEK"
     gene            3750..6230
                     /locus_tag="PLE39_11975"
     CDS             3750..6230
                     /locus_tag="PLE39_11975"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE69093.1"
                     /translation="MAPAQESSMHPEDKSQKRVEKLFSDLEQIAGQAVPSSGDSAAAR
                     PTSFPVRQEAPPVDPVVTALTARIHELEAKLEESNLRIAMAEAMLATEKTETARIPAI
                     VYEKEKVGYAFKDENILPLRDAQIAAKELEDAVDAPLIMGGDVIGDIKIEPPSDRPWT
                     EEEERLASAVAQQASLQIQNLRLLSATERARTEAQAATRRFTHESWESFLDGIRNSER
                     IGYLYDQSAVEPVRDIPEHDHRETVKVLDEQIGNLFIKSDPARPLSEEDKEMVAAIAR
                     QVSQQVENLRLLADASRARADAEDATRRLSRESWQSYTQRNQSSLGYVYDSIQVSPLT
                     DEFSNAENISLVQPLTVRGETIGRLVTLAPQTHAGGATPNRITPEAEGLAAQIAERVS
                     EQLERLRLTEEIQKRAAELATVATVSTAASTVLNPDELLQQVVDLTKERFGLYHAQVF
                     LADEAWNTLLLAAGSGDIGKQMVMSGNTIPMEKNELLVSRATRERKALISNDVRREES
                     FQLNPLLPDTRSEMAIPMIAGDKVLGVFNVHSVNLNHFTEEDANIYTTLASQVAVSLQ
                     NARLYAEQAATVAQLRELDRLKSSFLANMSHELRTPLNSILGFSDVMLEELDGPLTEN
                     MRNDLGLIQKNGQHLLHLINDVLDMAKIESGKLNLNIEKFNLQEVIEEVTSITAPFAS
                     EKNLSLFLEQDSDNEVQISADKIRIRQVMINLVNNAIKFTERGKISIRAACEDNHVLI
                     SVKDTGIGIPHDHLDSVFQEFTQVDTSTTRKAGGTGLGLPISRRLIEMHGGKLWAESS
                     GINGEGSIFYVFLPIEARLRTEEELKVK"
     gene            6235..7878
                     /locus_tag="PLE39_11980"
     CDS             6235..7878
                     /locus_tag="PLE39_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNE69094.1"
                     /translation="MAKPQFLQCAICGNQQPYEPFVPAVCKSCGSQWLEAKYDYAVFK
                     RNILRGLPDRPRNLWRFQDVLPLENPSALDLYPAGGTPLWLSQRFATKLGHDRVYFKD
                     ERYGPTSSFKDRQAAVAVAAMNENNIREAVIASTGNAAVAYAAACARAGIKLWVFMTS
                     LVPQEKLREAALFGAEVIRVSGNYDQTKQIASQFAQRRNLHLDRGASSIAARESMKTI
                     SYEIVEQLGWRAPDWYIQAVSGGMGPLGVYQGFKEMLDMGLIDRIPKIAIIQAEGCSP
                     MVKAFKADKEVADPVVPDTRIIILSTGDPGKTYTYLYGILKKHGGTMESVTDAEAFDA
                     MRSLAKSEGMAVEPATAVAYAGLEKMLREKIITKDETVVVNCTGHTFPVEKHVLGDQW
                     AVDVHLSKDQSPAPQEGLQAALENLDEKATTVLLVDDNSDDALLIRRLLEGKKSYRVY
                     HAKDGWEGLAMARQHLPDLIISDLTMPGIDGFGFVEELKLDPRTKEIPVVVVSAKDIT
                     AEERQRLNGRIEALYQKGSLPTRKFVDKVINVIEDKNGA"
     gene            7893..8282
                     /locus_tag="PLE39_11985"
     CDS             7893..8282
                     /locus_tag="PLE39_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE69095.1"
                     /translation="MGNNVLYIEDNPDNMMLVRRALESRGYRLVQALTGLDGVNKAET
                     EEVDLILLDINLPDIDGYEVARRIRSSDKKHALAYVPIIAVTANALKGDAEKALSAGC
                     DVYMSKPINIRELWARVEAFVPSPNSK"
     gene            8317..9492
                     /locus_tag="PLE39_11990"
     CDS             8317..9492
                     /locus_tag="PLE39_11990"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNE69096.1"
                     /translation="MKTSYLTDLTCSDCGRVFSINEVLTFCPDCQSTLISNYDLNAAR
                     ANVDRDEIHTRSKGMWRWHEMLPIQDPENIVTLGEGDTPLLRLPRVGNELGLSNFFVK
                     DESSNPTGSFKARGLCAAVSRAKELGVKKVIIPTAGNAGGAMAAYAARAGLQAMIYMP
                     KDTPNANIQESRIAGAEVILVEGLISDAAGMAGVKAREEGWFDVSTFKEPYRAEGKKI
                     MGYELAEAFDWTLPDVILYPTGGGTGLVGMWKAFAEMEALGWLKNTKRPRMVSVQAEG
                     CAPIPKAFHAGASFCDFWLGAQTVASGLRVPKSFADRIILSNIRESNGTAVAVSDEAL
                     MQAQKKLAAMEGIFAAPEGAACLAALEELVKQKWVQPDEKIVMFNTGSGLKYLDTMN"
     gene            complement(9712..10602)
                     /locus_tag="PLE39_11995"
     CDS             complement(9712..10602)
                     /locus_tag="PLE39_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69097.1"
                     /translation="MKRAVGISLGSSKRDKKVTVRLNQVEIQVERIGTDGDVEKARQM
                     YLDLDGKVDAFGVGGVDLYLRLDQKEYPLHAALKLVSGVKQTPLCDGRGLKHTLERRV
                     FQLARSTLGEIHFKQAFVPVAVDRLGLAEAVAEVSERTVFGDLMVALGVPIPVYGIPA
                     FKRVARVMLPMVSHFPMSMIFYGSDGAEHEPKYVKYFEESDLIAGDFLFMRKYMPKSL
                     AGKTVVTNTTTEENIALLKERGVKTVITTTPRYDGRSFGTNTTEAMLTAYAGKGRRLT
                     DDELNGLIDELGLKPNVIQF"
     gene            complement(10651..11790)
                     /locus_tag="PLE39_12000"
     CDS             complement(10651..11790)
                     /locus_tag="PLE39_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNE69098.1"
                     /translation="MKNPNAQNQKTNSAPSTPPSNPNPRPSFFSVIPGWVVGLVVAVI
                     FVALLASVSKSFWYFEVIENDQVGITIEAGEIQSVLQPGIAYDFGLFVDLVKITTSAV
                     AITVDDPELITIDKQRIGLEVTADVFRPREADIVISNYSRYRNIYLNDESLQQRMTAF
                     TLQAMKVCVGDKKFDEAVIGSGRDDLRACIDDELSGLAEPLGLEVRNVAVPQITISPE
                     VQAGLDAIVQSRLATEKAKQDAEKAKQEAIAQQAVEEGRIRVDQAKLQEEARQQAILQ
                     DIKRQQLEAELAVIEQEQINAQAKLELTDLLQQVADEQALVDIAKELALAALYEKYPS
                     FVTLQIALANASAIKPTDKFIFTQEGVFPNLIFSNGTLPTFNVNP"
     gene            complement(11827..12465)
                     /locus_tag="PLE39_12005"
     CDS             complement(11827..12465)
                     /locus_tag="PLE39_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE69099.1"
                     /translation="MLEIVSFTLGPAQTNAYLVADPDTKEAVVIDPSWDGHIILAEAQ
                     KRGWRIGHLWYTHAHFDHIGGAAAIADALNPLPHVALHPDDHVLWRAGGGGAKFGLNI
                     DPGPEPTIDFVQGMLLKLGSNEFEVRYTPGHTKGHCVLYVASENICFCGDVIFNGSIG
                     RTDLPGGDFATLENSIHTQIYTLPDETRLLSGHGPETTVGHEKMYNPFVRTG"
     gene            complement(12458..13585)
                     /locus_tag="PLE39_12010"
     CDS             complement(12458..13585)
                     /locus_tag="PLE39_12010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE69100.1"
                     /translation="MNRNLTFVVVSLLFWGIGEGMFLNFVPIYLGKEFALGEEQIGLA
                     LGMFGFFSAITHMPSGYLADRIGRRPLLITAWILGLASTFTMGISTALPFYLVGLFGY
                     GLTAFVSSPLSSYVTAARGEMPVGTALSLVSASFNIGMALGPVTGGWIGDHYGMRMSY
                     LVAAVIFLFSVASIFFIKNQPLDKHDPEAPPVTLWKNRGFISFLGMVGFAVFAMYLAQ
                     PLTPNFLEGVRKFSLSDTGWIFSAGALGNALFAIGFSRLDPRRGFLFAQVLVVLFALI
                     MWKGFGLPLFMLGYFLLGGFRAARPMAQAQGRELVHQSQMGLMYGTLETVNATIFIFT
                     PPLAGYLFVIDPFLIYPLAMGLITLAMISTFIFSYRKVQHA"
     gene            complement(13601..14392)
                     /locus_tag="PLE39_12015"
     CDS             complement(13601..14392)
                     /locus_tag="PLE39_12015"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE69101.1"
                     /translation="MNTQIIPTTEPFFLPGGRTGVLLIHGFTGAPKEMRWMGEYLNGQ
                     GYTCLGIRLHGHATTPEDMIASRWTDWTASVEDGFHLLSGSVDRIFFAGLSMGGVLSL
                     LMSTKFADRVSGVVAMSTPYSLPNDPKDYSPAYIRLYSHFVKYMPKSGEVPGASWFDK
                     EAFKDHISYPQNPIRSIAELKLLLGEMQAALPRLTMPVLLIHSKDDPYVLPENVEKIY
                     AGLVNASDKTKLLVTGSGHVVTRDAARHQVFEAARDFIRRVENQS"
     gene            complement(14389..14823)
                     /gene="aroQ"
                     /locus_tag="PLE39_12020"
     CDS             complement(14389..14823)
                     /gene="aroQ"
                     /locus_tag="PLE39_12020"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNE69102.1"
                     /translation="MKILILHGPNLNLLGTREPEVYGSTKLHELNESLIALGKEFNAE
                     VTCLQSNHEGALIDALHEARTQANGVVFNPGGYTHTSVALRDAISAIQIPVIEVHISN
                     VHAREEFRHKSMISAVCKGTVSGFGLKSYELGLRGLIDIIRS"
     gene            complement(14826..16364)
                     /gene="aroB"
                     /locus_tag="PLE39_12025"
     CDS             complement(14826..16364)
                     /gene="aroB"
                     /locus_tag="PLE39_12025"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNE69103.1"
                     /translation="MTHIFLYGPPGTGKSTVGRKLAQNLNLPFIDLDRVIEEEAGMSI
                     PQIMEQQGEGTFRDLETASLKKLAGEQESIVALGGGALLRDVNRAFAEQHGRIILFMA
                     ELSTLLERLHYEAGKRPLLAGDLKPKLTALLEKRREHYHSFPLMIHVDGKSAEQNAYQ
                     AQLAAGRHHLSAMGEYDAVVQSRGIDQLGEMLKTRGLQNPILVTDDNVANLHAEKAVT
                     ALRNSGFDPRVLTVPAGEAYKKLETISQIWKGFLEAGLDRKSTVIALGGGVIGDMSGF
                     AASTYMRGIAWVGVPTTLLSMVDASLGGKTGFDLPEGKNLIGAFYPPKLVLADPQLLD
                     TLPEAELISGMAEVVKHGIISDPELFSLCGRGLDWVKANLEEVVRRGMAVKIKVIEED
                     PYEKGVRAALNLGHTVGHAVELVSRFNLRHGEAIAIGTVVEARYSAQIGRAGHGLVDA
                     VTSSFKSLGLPVQIPDAMPRDEIIRAMRVDKKKTAKAIRFALPVEIGKVELVNVDDLE
                     SVLE"
     gene            complement(16468..>17519)
                     /gene="aroC"
                     /locus_tag="PLE39_12030"
     CDS             complement(16468..>17519)
                     /gene="aroC"
                     /locus_tag="PLE39_12030"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNE69104.1"
                     /translation="QGYGSGGRMKIEKDTVQILGGVMGGETTGAPLALLVQNDDHVKW
                     KGKAIEPMTAPRPGHADLTGAVKYGYKDLRPALERASARETTMRVAAGAVCKHFLAQF
                     GIIVGGYVASIGEVTADFGDMPYEERFTRAEASDVRCPIDSSAEKMRAEIEKTIHGKN
                     TLGGVLEIVALNLPVGLGSFVQWDRRLEAKLAMAIMSVQAIKGVEVGDAFENAKRIGT
                     EAHDPINLQPSTFDLQRSTNRAGGTEGGVSNGQPIIIRAAMKPIATTLVPQQTVDLAS
                     GENAPTKYERSDFCPVPRAVPILESMVAFVLADALLEKIGGDSMNEMKSRFETLRKAT
                     LEDLPMDNIPHIFWE"
     assembly_gap    17520..17619
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17620..17795)
                     /locus_tag="PLE39_12035"
     CDS             complement(<17620..17795)
                     /locus_tag="PLE39_12035"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNE69105.1"
                     /translation="MLRFLTAGESHGPSLTTILDGLPAGLSLSPDIINKELARRQQGY
                     GSGGRMKIEKDTVQ"
     gene            complement(17846..18679)
                     /gene="aroE"
                     /locus_tag="PLE39_12040"
     CDS             complement(17846..18679)
                     /gene="aroE"
                     /locus_tag="PLE39_12040"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNE69106.1"
                     /translation="MIRLGLIGYPLGHSLSPKIHAAALKACNLQGDYSLFPIHPEDKP
                     ALQALLARVRAGEITGLNVTIPHKQNVIEFMDELTPTAQVIGAVNTIYMRGDKLIGDN
                     TDAPGFLSDLKRVSNSTFSTPHSAIVLGAGGSARAVVYALLNDGWNVTIAARRIEQAQ
                     QLASSFTNYQLQVADFSSFSIQHSSLLVNTTPLGMTPNTETSPLPENTVLTKNTFVYD
                     LVYNPRETKLVRDAHAQGLNASTGLGMLIEQAALAFEKWTGHNPPRDVLFESVGATRP
                     D"
     gene            complement(18676..>18975)
                     /locus_tag="PLE39_12045"
     CDS             complement(18676..>18975)
                     /locus_tag="PLE39_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69107.1"
                     /translation="DSPTRIRGIASARVKETDRVHATCTELARLGVQVEEHEDGMTIH
                     PTFNLQPANIQTYNDHRMAMAFSLIGLKFDGVTIENPSCVSKTFPNFFEVLETLR"
BASE COUNT         4775 a         5082 c         4720 g         4298 t
ORIGIN      
        1 caaaccgcca tccgcgaact tgggcttgca ttgggtgcaa cccgcgtcaa agcagcgatc
       61 tccgcccgta ataacagcga taacaaaaaa taaaaaagcc tccttttgat atttgccaac
      121 aaggaaccgc atcgggaaga agacctgaac tacccgatga aggagaaaca aaaatggata
      181 acaaaaaaac atatccgctc catctggacg atgaaagccg ccaacacaca cgttggggca
      241 caattctcct gtcaaccata ttggtgttgg tgtacgccta tgtcctgaca caaatattta
      301 ttggagaaaa gaccacccaa agttacattg ccatggcggt cctcctgctg gtctcaacat
      361 ctactgtgat gggtctggtc ctttccattc gtcagcgcac agaactgggt ctgaagctga
      421 cctattactc gttgatcgca ttatgtgttg ctgcagtcgt tttatttcaa ggccgcacaa
      481 cctcgattac tttttccatt ctgagcattt caaccgttgg tttcatctgg ctattcccgc
      541 agcatacccg ccgtgaatat gcaaccatca tgatcgtttc gcttgtggca atctgggtga
      601 ctgaattggc aaacccggcc tggcgtctgt ccatgaacat ggcccaggtt ggcccggcag
      661 gggcaggtat ttttgtcatt ctctttgcga tctacatatt tagagaatca cagaatgtca
      721 tttcctccaa tcttcgcctc aagatcacca tctggacagg cctgatcctg acaggtattt
      781 ccatcatcct gatcgtttac tctcaaatca ttgcccgtca ggctgccgtt gatgcggcag
      841 aaaaagaagc gttgtccttc gctgaatcac aagcacgttc agtgcgtgca gatgttgaaa
      901 ttcccttgga cacagcacgt gcgctcgcac atgcgctgac ctccaccaag gaaccatcca
      961 atgatgaagt tctaagccgc actcaagtca acgccatgct gcgccaggtc cttcttgaga
     1021 atccaaactt tttgggaacc tacaccctct gggaaccaaa cgcatttgac ggactcgatc
     1081 aaaccttcaa aggcaccgca gcccatgaca acacaggccg cttcatcccc tactgggtcc
     1141 gcggcgatga tggaagcctg accgtaacgg ctctgttgga ttacgaaacc ccaggcttgg
     1201 gtgattggta cctgataccc cgccaaaccc aaaaagaagt gaccattgcc ccgctcatct
     1261 acccaattca aggcgtggat acggtaatgg catcttttgt cgtaccaatt atttatgctg
     1321 ataaattcta cggcatcgca ggtgtggatg ccccgatcgc ctttgtacaa aatctggtgg
     1381 accaggtcga tctttacaac gggaaagctg gtgcagtctt aatgacatca gacggcaccc
     1441 tgatcagcgt ccgcaaccag cctgaattgg tgaaccaacc cgccgcagaa atatttcccg
     1501 atttcctgga gttacagtca cgcattcaag caggtgaatc cttcatcagc ctgagcccgg
     1561 acggggattc tttacgcgca ttcgcggcgg ttgacctcgg ccaaacgggc gatcactggt
     1621 cgtttgtgct gcttgttcca ttttcagaga tcatagcccc agccaacaca gccgccattc
     1681 gtcagggtgt gatcggtctc gtgctcatcg caaccgccct tgctattcta tggttcttaa
     1741 gcggccagat cgtacgcccg gtacgcgaac ttacaactgt tgcaaatgca atttcgcaag
     1801 gcaacctcaa cgtcactgca agagtgcagg ccgccgatga aacaggtctg ctcgccagca
     1861 ccatcaattt gatgatcacc caattgcgcg aatcattcgt cacgctggaa gaacgcgtgg
     1921 cagaacgtac acaaaacctg gaactggcct cacaagtcgg tcgctctgta tcgcagcttc
     1981 gaaagctgga tgtgatgtta aaggatgcgg ctgagatcat ccgctccagg tttgatcttt
     2041 actatgttca ggtatatctg acaaacccca gcaaaaccta cctcaacctg caggctggta
     2101 ctggccacgt gggagaggaa ttgctggctc gcaatcaccg cctgcccatc aacacaggct
     2161 cgatcaacgg tcgcgcagcg gtcgaaaaga gatcggtgat catcgctgat acatccacca
     2221 gcactacctt ccgccccaat ccccttctac cgaacacccg ctctgaaatg gccgtcccct
     2281 tgatccttgg tgaaacagtg gtgggcgtgt tggatatgca gagcgaaaca gcaggtgcat
     2341 tgaatcacga aatgttatcc gcatttgaag cattggctgg tcaattggcc atcgccattc
     2401 aaaatgcaaa cctgctggca gaagccgagc aggcacgtgc cgaagtggaa gcgcaggcac
     2461 agcgcctcac ccgcacaaat tgggcaagtt atctggatgc catccacaag cccgaagagt
     2521 ctggttatgt atttgaacag aacaaggtca tgccaatggt tgaggcagag gatcaaacag
     2581 tgaacgatga tgcactcact gccccgatcg ccataactgg agaaaccatt ggaaacctgg
     2641 tggtggaatt ggaaggccag tctccgctta cccgtccaaa tgaattgatc aacattgtgg
     2701 cacgccaggt tgcccaacaa attgaaaacc tgcgcctgct cgaaaatgcc gaacgctacc
     2761 gcgttgaggc tgaagaagcc tcccgccgcc tgatccgcga aggttggaaa acctataaag
     2821 agggtgcgaa aaacaacctg agctatctat acgacctcaa ggaagtacgc ccgctgaatg
     2881 gagacagcac atcagaacct gcactttcca ttccgcttaa agtccgtgat gagttgatcg
     2941 gcgaactggc cattcaggga atgaatctgg aagatggaga ctcagccagc cttgccgccg
     3001 ctgttgcgga tcgtctcagc tcccatattg aaactttgag gcaatacgat caggcacaat
     3061 cggcgctcga acaatcagaa aaactgtttg atgccagccg ccgcctgacc caggcgacca
     3121 gcctgcaaga cctggtagcc gccacggtag aatcgcttga tattccagtt gtcaaccgcg
     3181 ctgtattggg aatcttcaac tacggagcag acgataaact ggagagcatg tccatcgaag
     3241 caaattggtg gaatggtaca ggccacaatg tcactgccat tggtgtgcgt tatcctgtgg
     3301 cagtcttccg cacggtttca ctctttgcca gcccggtacc gctctttttt gcagatgcat
     3361 tcaatgatga acgtgccgat gcagccacat tgcagcttgc caaacaattg aatctgcgtg
     3421 cggtggcggt tctgccgctt tacatcggta cccgtcaggt gggtgtactc atgcttgaag
     3481 cagaacagcc ctaccatttc agtcaggatg aaatccgtct gttctcctcg ctggcgccgc
     3541 aaattgccac cgtgttggaa aacagacgcc aattcgaccg tgcacaacaa caggctgaac
     3601 gtgaaagtac cttgaacctc atcagccaga agatccaaag cgcaaccaca gtggatgccg
     3661 tgcttcagat cgccgcccgt gaattggggc acgccctcgg cgcccccatg accattgctc
     3721 aactgagcat gaaggataaa gaaaagtaaa tggccccagc acaggagtcg agtatgcacc
     3781 ctgaagataa atcacaaaag cgggtggaaa agctcttctc ggatctggaa cagatcgcgg
     3841 ggcaggcagt cccttccagc ggggattctg ccgcagcacg cccgaccagt ttccccgtcc
     3901 gacaggaagc gccgcccgtt gatcctgtgg taaccgcgct gactgcacgc atccatgagt
     3961 tggaagcaaa actcgaagag agcaatctgc gcattgccat ggctgaggcc atgcttgcca
     4021 cagagaaaac agaaacggcc cgcatccctg ccattgtgta tgaaaaagaa aaagttgggt
     4081 atgccttcaa ggatgagaac atcctgccgc ttcgagatgc acagatcgcg gcgaaagaac
     4141 ttgaagacgc agtggatgcc ccgttgatca tgggcggaga tgtgattggc gatatcaaga
     4201 tcgagccgcc cagtgaccgc ccgtggacgg aagaggaaga acgcctcgcc agcgccgttg
     4261 cccagcaggc atcactccag atccaaaacc tccgcctgct ttcagcgaca gaacgcgcac
     4321 gcaccgaagc acaggctgcc acccgtcgtt ttacccatga aagttgggaa tcgtttttgg
     4381 atggcattcg caacagcgaa cgcattggat atctgtacga tcaatctgca gtggagcctg
     4441 ttcgtgatat tcccgaacat gaccacaggg aaaccgtgaa ggtgcttgat gaacagatcg
     4501 gaaacctgtt catcaaatca gaccccgccc gcccattgag cgaggaagat aaggaaatgg
     4561 tcgccgcgat cgcgcgccag gtttcgcaac aggttgaaaa cctgcgcctg ctggcagatg
     4621 cctcccgcgc ccgcgccgat gccgaagatg ccacccgccg cttaagccgt gaaagctggc
     4681 aatcgtacac ccagcgcaat caatcttccc tcggttatgt atatgactcg atccaggtca
     4741 gcccgctgac agatgaattc tccaatgctg aaaacatcag ccttgtacag ccgttgacgg
     4801 tgcgcggcga aaccataggt cggcttgtaa cactcgctcc acagacccat gcaggcggag
     4861 cgacgccaaa ccgcatcaca cctgaagcgg aggggcttgc cgctcaaatt gccgagcgtg
     4921 tcagtgaaca actggaacgc ctgcgcctga cagaggaaat tcaaaaacgc gccgcagagc
     4981 ttgccacggt ggccacagta tccaccgcag catccactgt gttgaatcca gatgaactct
     5041 tgcagcaagt cgtagacctg accaaagaac gtttcggctt gtaccacgca caggtcttcc
     5101 ttgctgacga agcctggaac accctgttgt tggccgcagg ttcaggagat atcggaaaac
     5161 agatggtgat gtctggcaac accattccga tggaaaagaa tgaactgctt gtctcccgtg
     5221 ccacacgtga acgcaaagcc ctgatctcga acgatgtgcg cagggaggaa agtttccaac
     5281 tcaatcccct gctgcccgat acccgctccg aaatggcgat cccgatgatc gcaggcgata
     5341 aggtgctggg agtgttcaac gtccattctg taaacctcaa tcactttaca gaagaagatg
     5401 ccaacatcta caccacactg gcttcacaag tggcagtgtc actgcaaaat gcgcggttgt
     5461 atgcggaaca ggccgcaacc gtggcgcaat tgcgcgaatt ggaccgcttg aaatcatcct
     5521 tccttgccaa tatgtcgcac gaattgcgca ccccgctcaa ctccatcctc ggtttttcag
     5581 atgtgatgct cgaagaactc gatggtccgc tcacagagaa catgcgcaac gatctgggcc
     5641 tcattcaaaa gaacggccaa catctgctcc acctcatcaa cgacgtgctc gacatggcaa
     5701 agatcgaatc aggcaagttg aacctcaaca tcgaaaaatt caacctgcag gaagtcatcg
     5761 aagaagtgac cagcatcacc gccccgttcg ccagcgaaaa gaatctatcg ctcttcctcg
     5821 aacaggattc agacaacgaa gtgcagatca gcgccgacaa gatccgcatc cgtcaggtga
     5881 tgatcaacct tgtcaacaat gccatcaagt tcaccgaacg cggcaaaatc tccatccgtg
     5941 ccgcgtgtga agataaccac gtgcttatca gcgtcaaaga tacaggcatc ggcattccgc
     6001 acgatcacct cgatagcgtc ttccaggaat ttacacaagt cgacacttcc accacccgca
     6061 aagcaggggg cacggggttg gggctgccca tcagccgtcg cctgatcgaa atgcacggcg
     6121 gaaaattatg ggctgaaagc agcggcatca atggtgaagg ctccattttc tatgtgttcc
     6181 tgcccattga agcaaggctc agaaccgagg aagaattgaa ggtaaaataa cggcatggcc
     6241 aaacctcaat tcctgcaatg tgccatttgt ggcaaccaac aaccttatga accttttgtc
     6301 cctgctgttt gcaaatcctg cgggtcgcaa tggcttgaag caaaatacga ctacgcggtt
     6361 ttcaaacgca acatcctgcg cggattacca gaccgcccca gaaatctctg gcggtttcag
     6421 gatgtcctgc cgcttgagaa tccctccgct ttagacctct accccgcagg cggcacaccg
     6481 ctctggcttt cacagcgctt cgcaaccaag ctggggcatg atcgggtgta tttcaaggat
     6541 gaacgctacg gcccgacaag ttccttcaaa gaccgccagg cagccgttgc agtcgcggca
     6601 atgaatgaaa acaacatccg tgaagcggtc atcgcctcca cgggaaatgc cgcagtggct
     6661 tatgctgcgg cgtgtgcgcg ggcgggtatt aaactttggg ttttcatgac cagcctggta
     6721 ccgcaggaaa aattgcgcga ggccgccctt tttggtgcgg aggtgattcg cgtcagcggc
     6781 aattacgacc agaccaaaca gatcgcatcg caatttgcac agcgccgcaa cctgcatctt
     6841 gaccgtggcg caagttccat tgccgcccgc gaatcgatga agaccatctc ctacgaaatt
     6901 gtggaacaac tcggctggcg cgcgcctgat tggtacattc aagcggtcag tggtgggatg
     6961 gggccgttgg gggtgtatca gggcttcaag gaaatgctcg atatggggct gatcgaccgc
     7021 attcccaaga tcgcgatcat tcaagccgaa ggctgttcac cgatggtcaa agccttcaaa
     7081 gccgataaag aagtggccga ccccgtggtg cctgataccc gcatcatcat cctctccaca
     7141 ggtgacccgg gcaaaaccta tacctatttg tatggcattc tcaaaaaaca cggcggcacc
     7201 atggaatctg tgaccgacgc tgaagccttc gacgccatgc gttctctcgc taaaagtgag
     7261 ggaatggccg tcgaacccgc aacagccgtc gcctacgccg gactcgagaa aatgctgcgt
     7321 gaaaaaatca tcaccaaaga tgaaacggtg gtcgtcaact gcacaggcca caccttcccc
     7381 gttgaaaaac atgtcctcgg cgatcaatgg gctgtggatg tccacctcag caaggaccaa
     7441 tcccctgctc cacaggaagg cctgcaagcc gcgcttgaaa atctggatga aaaagcaacc
     7501 acggttctgc ttgtggatga caattccgac gatgctttgc tcatccgccg tttgcttgag
     7561 ggtaaaaaat cctaccgtgt ttatcacgcc aaggatggct gggaagggct cgccatggca
     7621 cggcagcatc tgcccgacct gatcatcagt gatctgacca tgcccggtat tgacgggttt
     7681 ggctttgtgg aagaactaaa acttgacccg cgtacgaagg aaattcctgt tgttgtcgtc
     7741 agcgcaaaag acatcactgc agaagaacgt cagcgcctca atggacgcat tgaagcgcta
     7801 tatcaaaaag gttcactacc cacccgtaaa ttcgtggata aggtcatcaa cgtcatcgaa
     7861 gataaaaacg gggcctaaag gagcaaacga gaatgggaaa caacgtttta tacattgaag
     7921 acaatccaga caacatgatg ctggtcaggc gcgcccttga atcacgcgga taccgactgg
     7981 tgcaggccct gacgggtctt gatggagtca acaaggctga aacagaagag gtggatttaa
     8041 tcctgttgga tatcaacctg cccgatattg atgggtacga agtagcccgc cgaattcgca
     8101 gcagtgacaa aaaacacgcc ctggcttatg tgcccattat tgccgtaaca gccaatgcgc
     8161 tcaaaggtga tgcagaaaag gcactctctg caggatgcga tgtgtacatg tccaaaccca
     8221 tcaacattcg cgagctttgg gcacgtgtcg aagcatttgt tccatccccc aattcgaaat
     8281 aaacaattca aaatcactac ttacaggttg aatattgtga aaacatccta tcttaccgat
     8341 ctgacttgtt cggattgtgg aagagtcttt tccatcaatg aagtccttac attttgccct
     8401 gattgccaat ctacactgat ttcaaactac gatctgaatg ccgcccgcgc aaatgtggac
     8461 cgtgacgaga ttcacacgcg ctcaaaaggg atgtggcgct ggcatgaaat gctccccatt
     8521 caggatcctg aaaatatcgt aactctcgga gagggagata cgcccttgct acgcctcccc
     8581 cgcgttggaa atgaacttgg gctttccaat ttcttcgtca aagacgagag cagcaacccc
     8641 acaggctcat tcaaagcccg tggactttgc gccgccgtat cccgcgcaaa agagctcggc
     8701 gtcaaaaagg tcatcatccc caccgcaggc aatgcaggcg gagccatggc cgcgtatgcc
     8761 gcgcgtgctg gcttgcaggc aatgatctac atgcccaaag acacacccaa tgcaaatatt
     8821 caggaaagcc gcattgcagg cgccgaggtc attcttgtgg aaggcctcat cagtgacgcg
     8881 gctggtatgg cgggtgtcaa agcgcgtgaa gaaggttggt ttgatgtttc cactttcaag
     8941 gaaccgtatc gcgcagaagg caaaaagatc atgggttatg agctcgccga agcttttgac
     9001 tggactctgc ccgatgtcat cctctacccc actggcggcg gcacgggtct ggtgggcatg
     9061 tggaaagcct tcgcagaaat ggaagccctt ggctggctga aaaatacaaa acgtccgcgc
     9121 atggtttcgg tgcaagcgga aggctgcgcc ccgattccca aggcctttca tgctggcgct
     9181 tcgttctgtg acttttggct gggtgcccaa actgtggcat ctggcttgcg tgtgccaaaa
     9241 agcttcgccg accgtatcat cctctcaaat atccgtgaaa gcaacggcac agcggtagcc
     9301 gtcagcgatg aagccttgat gcaggcccag aaaaaactcg ccgccatgga aggtatcttc
     9361 gccgcccctg aaggtgctgc atgtctggcg gcccttgagg aattggtcaa gcaaaaatgg
     9421 gttcagcccg atgaaaagat cgtcatgttc aacacagggt cggggttgaa atatctcgac
     9481 acgatgaatt agaaacacaa aaaagccgat ccattttaca gggtcggctt tttatatctc
     9541 gtctgtgtct tgcaattcat ttgacctctt gcataagtta cgttgcaagg aatgtctttg
     9601 aaaagctgtt ttgaaccact gaggtttctc tgtgtcttcg tggctcagtg gttgatcttt
     9661 cagacttttg caaaattcgc gactgattcc ttaagttttt tggaatcatg tttaaaactg
     9721 gatcacattt ggtttcaacc ccagttcatc gatcaaacca ttcaattcat cgtcggtcag
     9781 cctgcgtcct ttgcctgcat aggcggtgag catggcttcg gtggtgttcg tgccaaagga
     9841 tcggccgtca taacgtggcg ttgttgtgat gaccgtcttc acgccgcgct ctttgagcag
     9901 ggcaatattc tcttcggtgg tggtattcgt caccacggtt tttcctgcga ggcttttcgg
     9961 catgtatttt cgcatgaaaa gaaaatcgcc cgcgatcagg tccgattctt caaaatattt
    10021 cacgtacttt ggttcatgtt ccgcgccgtc actgccgtaa aagatcatgc tcatcggaaa
    10081 gtgactgacc atgggcagca tgacccttgc gactcgtttg aaggcgggaa tcccataaac
    10141 gggaatcggg accccgagcg caaccatcaa atcaccgaac acggttcttt cgctgacctc
    10201 ggcaacagcc tcagccaacc cgagtcgatc caccgccaca gggacgaacg cctgtttaaa
    10261 gtggatctct cccagtgtgg atctcgccag ttgaaacacg cgccgttcaa gggtgtgctt
    10321 gaggccgcga ccgtcacaga gtggagtctg cttgacgcca gaaacaagtt tcagcgcggc
    10381 atgaagcgga tactcctttt ggtcaagccg cagatataga tccacaccac cgacaccgaa
    10441 ggcatccact ttgccatcga ggtcaagata catctggcgt gccttttcca catcgccatc
    10501 ggtgccgatg cgctcgactt gaatctcaac ttgattgaga cggacagtga cctttttgtc
    10561 acgtttcgaa cttccaaggc tgatgccaac agctcgtttc attttttact ctgaaaaaat
    10621 attggggtgg agaaactcca ccccaaacga ttatgggttc acgttgaatg taggcagggt
    10681 accgttagag aaaatcaaat tcgggaagac gccttcctgt gtgaagatga atttatctgt
    10741 gggtttgatg gcagaggcat ttgcaagggc gatctgcaaa gttacgaacg acggatattt
    10801 ttcatacaat gcagccagag ccaattcctt ggcaatatcc accaaagcct gttcatcggc
    10861 aacctgttgc aaaagatcgg tcagttccaa tttggcttgt gcattgattt gttcctgctc
    10921 gattacggca agttcagctt ccagttgttg ccgcttgata tcctgcaaaa tggcttgctg
    10981 tctggcctct tcctgcaatt tggcttgatc cacccgaatg cgaccttctt caactgcctg
    11041 ttgggcaatg gcttcctgtt ttgctttctc ggcatcctgc ttggcttttt cagtggctaa
    11101 acggctttgg acgatggcat ccaaacccgc ctgtacttcg ggcgaaatcg ttatttgcgg
    11161 caccgccaca ttgcgcactt caaggcccag cggttcggcc agcccgctca attcatcatc
    11221 aatacaggct cgcagatcat cacgccccga tccaatgact gcttcatcaa atttcttatc
    11281 acccacacaa accttcattg cttgcagggt aaaagcagtc atacgctgtt gaagcgattc
    11341 gtcattcaga tagatgttgc gataacgcga ataattagag atgacaatat ccgcttcacg
    11401 tggacgaaac acatccgcgg tgacctccag cccaatgcgc tgtttgtcga tcgtgatcaa
    11461 ttcagggtca tccacagtga tcgcaaccgc gctggtggtg atcttgacca gatccacaaa
    11521 caggccgaaa tcgtaggcaa tgccaggttg cagcacgctc tgaatttcac ccgcctcgat
    11581 cgtaatgcca acttgatcgt tctcgatcac ttcaaagtac cagaaagact tgctcacact
    11641 ggcaagcaag gcaacaaaaa taaccgccac caccaaaccg accacccaac cagggatcac
    11701 agagaagaat gaaggcctcg gattgggatt ggaaggtggt gttgatggag cagaattcgt
    11761 tttttggttt tgagcatttg gatttttcat ttttacctcc tgttgattgg acaaattata
    11821 agggaatcac ccagttctta caaagggatt gtacattttt tcgtgcccca ctgtcgtttc
    11881 aggtccatga ccagaaagca ggcgggtttc atcgggcagt gtgtaaatct gagtatggat
    11941 gctattttcc agcgtggcaa aatcgccgcc gggcaggtct gtgcggccaa tgctgccatt
    12001 gaagatcaca tcaccgcaaa agcagatatt ttcgctggca acatataaaa cgcaatgacc
    12061 tttggtatgc ccgggcgtat aacgcacttc gaactcgttc gaaccgagtt tgaggagcat
    12121 gccttgcaca aaatcaatgg ttggctcggg gccagggtca atattcaagc cgaactttgc
    12181 cccgccgccc cccgcccgcc agaggacatg gtcatcggga tgcagggcca catgcggcag
    12241 cggattcaag gcatcggcaa tcgcagccgc gccgccgata tggtcaaaat gtgcgtgtgt
    12301 gtaccaaaga tgaccgatac gccagccgcg tttttgcgcc tctgccagaa tgatatgccc
    12361 atcccacgat ggatcgatca ccacggcttc ttttgtgtca gggtcagcca ccagataggc
    12421 attggtttgt gcggggccga gggtaaagga aacgatctca agcatgttgc acctttcgat
    12481 aggaaaaaat aaatgtagat atcatcgcca gcgtaatcaa acccatcgcc agcggataga
    12541 tcaaaaacgg gtcaatcaca aaaagataac cagccaacgg cggcgtaaaa ataaaaatgg
    12601 tcgcattgac ggtttcaagc gtgccataca tcaaacccat ctgcgactga tgcacaagtt
    12661 cacgcccctg cgcctgtgcc attgggcgag ccgcacggaa accacccaga agaaaatacc
    12721 ccagcatgaa caggggcagg ccgaaaccct tccacatgat caatgcaaaa agcaccacca
    12781 gaacctgcgc gaacaaaaat cccctgcgcg gatcaaggcg gctgaaaccg atggcgaaca
    12841 aggcatttcc caacgcgcct gctgaaaaaa tccaccctgt atcactcagt gagaatttgc
    12901 gcacaccttc cagaaaattc ggcgtgagcg gttgtgccag atacatggca aatactgcaa
    12961 acccaaccat gcctaaaaac gaaatgaagc cgcggttctt ccacaaagta acgggtggag
    13021 cctcggggtc atgtttatca agcggttgat tcttgatgaa aaaaatactc gcaacggaaa
    13081 acagaaaaat cactgccgcc accagataac tcatgcgcat gccgtaatgg tcaccgatcc
    13141 aaccgcctgt cacagggccg agcgccatgc cgatattgaa cgaagccgac accagcgaaa
    13201 gcgcagtgcc cactggcatc tcgcctcgtg ccgcagtgac ataactgctc aacggcgaag
    13261 agacaaaagc agtcaggcca tagccaaaca agcccaccag ataaaaaggc agagcggttg
    13321 aaattcccat ggtaaatgtg gaagcaagac ccaggatcca tgctgtaatc agcaaaggcc
    13381 gcctgccgat gcggtcagca agatagccgc ttggcatgtg tgtgatcgca ctgaaaaaac
    13441 caaacatccc aagagccagc ccgatctgct cctcccccaa cgcaaactcc ttacccagat
    13501 agatcgggac aaaattcaag aacatccctt cgccaattcc ccaaaacaaa agggacacca
    13561 ccacaaacgt cagatttcga ttcaaagttt cctcttggaa ttacgactga ttttcaactc
    13621 tgcggataaa atcccgcgcc gcttcaaaca cttgatgacg ggccgcatcc cgcgtaacca
    13681 catggcctga cccagtcacg agaagctttg tcttgtctga agcattgacc agcccagcat
    13741 aaattttctc cacgttttca ggcagcacgt acggatcatc tttggaatga atcagcaaaa
    13801 cgggcatggt caaacgtgga agagccgcct gcatttcacc gagcaacagc ttcaactccg
    13861 caatcgaacg gatgggattc tgtggatatg aaatgtggtc tttgaaggct tccttgtcaa
    13921 accatgaagc gccaggcact tcacccgatt tgggcatgta ttttacaaaa tgactgtaca
    13981 gcctgatgta tgcgggcgaa tagtcctttg ggtcattggg cagcgaatac ggcgtagaca
    14041 tggccaccac ccccgaaaca cgatccgcaa atttggtgga catcagcaga gacaatactc
    14101 cccccatcga aagccctgca aagaagattc ggtcgacact gcccgagagt agatgaaaac
    14161 catcttcaac agaagcagtc caatcggtcc agcgggaggc gatcatatct tcgggtgttg
    14221 ttgcatgacc gtggagtcga atccccaaac aggtgtatcc ctgcccatta agatattccc
    14281 ccatccagcg catttccttg ggcgcgcctg taaagccgtg gatcagcaat acccccgttc
    14341 gcccgccagg aagaaaaaaa ggttcagttg ttggaatgat ttgagtgttc acgacctgat
    14401 tatatcaatc agcccgcgta accccaattc ataagacttc aaaccaaaac cagagacagt
    14461 acctttgcac accgctgaaa tcatggattt atgccgaaac tcttcacgtg catgcacatt
    14521 cgaaatatgc acctcgatca caggaatttg aatggcagag atcgcatcgc gcagcgccac
    14581 cgatgtgtga gtgtaaccgc cagggttgaa caccacgcca ttcgcctgtg tccgtgcttc
    14641 atgcagggca tcgatcagcg caccttcatg attcgattgc aggcaggtca cttctgcatt
    14701 gaattctttt ccaagcgcga tcaagctttc attgagttcg tgcaacttcg ttgagccgta
    14761 cacttcaggc tcacgcgtgc cgagcaaatt caggttggga ccatgcaaga tcaatatttt
    14821 catctttact ccaaaacaga ttccaaatcg tccacattca ccagttcaac tttaccaatt
    14881 tcaacaggca gcgcaaaacg gatcgccttt gcagttttct ttttatccac ccgcatcgca
    14941 cggatgatct cgtcacgggg catggcatct ggaatttgca ctggcaagcc aagtgacttg
    15001 aaggatgaag tcaccgcatc caccaacccg tggcctgctc tgccaatttg agccgagtat
    15061 ctcgcctcaa ccactgtgcc aatggcaatt gcctcgccat gccgcaaatt gaacctgctg
    15121 acaagctcca ctgcatgacc aaccgtatgc cccaaattca aggccgcgcg gactcccttt
    15181 tcatacgggt cttcctcaat gactttgatc ttcaccgcca tcccacgcct gaccacttct
    15241 tcgaggttgg ccttcaccca atccaacccg cgtccacaca gagagaacaa ttcagggtca
    15301 gagatgatgc catgcttgac cacttcagcc ataccagaga tcaactcggc ttcgggtaat
    15361 gtatcaagca gctgtgggtc cgccagtacc agtttcggcg gataaaacgc gccgatgaga
    15421 ttctttccct cgggcaggtc aaagcccgtt tttccgccaa gcgaggcatc caccattgaa
    15481 agcaaagtgg tcggcacacc cacccatgca attccacgca tgtaagtgga ggcggcaaat
    15541 cctgacatat cgccgatgac accgccgccg agggcaatga ccgtgctttt gcggtccagc
    15601 cctgcttcga gaaatccctt ccagatctgg ctgatggtct ccagcttctt gtaagcctcg
    15661 cctgcaggga cggtcaacac tctgggatcg aaacctgaat tccgtaaagc cgtgacagct
    15721 ttttcagcat ggagattcgc aacattgtca tccgtaacaa ggatcggatt ttgcaatccg
    15781 cgtgttttga gcatttcacc aagctggtca attccgcggc tttgaaccac tgcatcatac
    15841 tcccccattg ccgagaggtg atgcctgcct gcagccagtt gggcctgata ggcgttttgt
    15901 tcggcagatt tcccatccac atggatcatc aatggaaagg aatgatagtg ctcccgccgt
    15961 ttttcaagca aggctgtcag tttcggtttc aaatctcctg ccagtagggg gcgctttcca
    16021 gcttcatagt gcagcctttc caacaaggtg gataattccg ccatgaaaag aataatcctg
    16081 ccatgttgtt ctgcaaaggc gcgatttaca tcacgtaata aagcgccgcc gccaagggcc
    16141 acaatacttt cctgttcgcc cgccaatttt ttcagcgatg cggtttcaag gtctcggaat
    16201 gtcccttccc cttgttgttc catgatctgc ggaatggaca tccctgcctc ttcttcgatc
    16261 acacgatcca gatcaatgaa cggcaggttc aggttttgag caagttttct cccaaccgtg
    16321 gatttgccag taccaggagg gccatagagg aagatgtgtg tcatgggggt gaaggtttca
    16381 ggtttcaggt ttcaggtttc aggttgaagg ttgaaggtag tgagtggagg gttgaatgcg
    16441 ggtgatagtc ggtggtcggt cgtcaaatta ttcccaaaaa atatgcggaa tgttatccat
    16501 cggcaaatct tccagcgttg ccttgcgcag ggtttcaaag cgcgatttca tttcattcat
    16561 tgagtcaccg ccgatctttt cgaggagcgc atcagctaaa acgaaagcga ccatcgattc
    16621 caaaatcggc accgcacgcg gaacggggca aaaatcggaa cgttcatatt tggtgggagc
    16681 attttcgcca gaggcaaggt caacggtttg ttgtgggaca agggtagtgg caatcggttt
    16741 catcgccgcg cggatgatga tgggctgtcc gttggaaacg ccgccttcgg tgccgcctgc
    16801 tctgttggtt gaacgttgaa ggtcaaaggt tgaaggttga aggttaattg ggtcgtgggc
    16861 ttcagtgcca atgcgttttg cattttcaaa tgcatcgccc acttccacgc ctttgatggc
    16921 ttgcacggac atgatcgcca tggcaagttt ggcttcaagg cgtcgatccc attgcacgaa
    16981 acttcctaaa ccaactggta agttcaatgc aacaatctca aggacaccgc caagtgtgtt
    17041 cttgccgtgg atagtctttt cgatctcggc gcgcattttt tcagcggagg agtcgattgg
    17101 gcagcgcaca tccgatgctt cggcgcgggt gaagcgttct tcgtagggca tgtcaccaaa
    17161 gtccgcggtc acttcaccga tggaggcaac gtatccgcca acgatgattc cgaactgcgc
    17221 gaggaaatgt ttacacacgg ctccagccgc gacgcgcatg gttgtttctc tggcagacgc
    17281 acgttcgagc gcggggcgca ggtctttgta gccgtatttg accgcacccg ttaaatccgc
    17341 atgacctggg cgtggagcag tcatcggttc aatggctttg cccttccatt tgacatggtc
    17401 atcgttttgc acaagcaatg caagcggtgc gcctgtggtc tctccgccca tcacgccgcc
    17461 gagaatctgc acggtgtctt tctcgatctt catgcgtccg cctgagccgt agccttgctn
    17521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tctgcacggt gtctttctcg
    17641 atcttcatgc gtccgcctga gccgtagcct tgctgacggc gggcaagttc tttgttgata
    17701 atgtctggtg agagggaaag cccagcgggg agtccatcga gaatggtggt aagtgatggt
    17761 ccgtgggatt cacctgcggt gaggaatcta agcataagtt ctccaaaaaa taggggcgac
    17821 ccatcatggt cgtccgaggt tttgattaat cagggcgggt cgcccctacg gattcaaaca
    17881 acacatcacg cggcgggttg tgacctgtcc atttttcgaa ggcgagggca gcttgttcga
    17941 tgagcatacc caagccagtg gaggcgttca gtccctgtgc gtgggcgtcg cggacaagtt
    18001 tggtctcacg tgggttatac actaaatcat aaacaaaagt atttttcgtt aaaaccgtat
    18061 tttcaggcag gggtgaggtt tcggtgttgg gagtcatgcc gaggggagtg gtgttgacaa
    18121 gcagtgatga atgctgaatg ctgaatgatg aaaaatcggc aacctgtaat tggtaattcg
    18181 taaatgaact ggctaattgt tgcgcttgtt caattcgtcg agcggcgatt gtgacattcc
    18241 agccgtcgtt gagcaaagca tacacaacgg ctcttgcaga acctcccgca cccaaaacaa
    18301 ttgcagaatg aggagtgctg aatgttgaat ttgaaactcg ctttaagtca gagagaaatc
    18361 ctggcgcgtc ggtattatcg ccgatgagtt tgtcgccacg catgtagatc gtgttgacag
    18421 caccgatgac ttgggcagta ggtgtgagtt catccatgaa ttcgatgacg ttctgcttgt
    18481 gcggaatggt gacattgagt cctgtaatct caccagcgcg gactcgggcg agtaaagctt
    18541 gtaatgctgg tttgtcttcg gggtgaatgg ggaataggga atagtcacct tgcaggttgc
    18601 atgctttgag agcggctgcg tggatttttg gtgacaacga atgtcccagt ggatacccga
    18661 tcaagccaag tcgaatcatc gcaaagtttc caatacttca aagaaattcg ggaaggtctt
    18721 agatacacat gatggatttt cgatggtcac gccatcaaat ttcagaccaa tgagggagaa
    18781 tgccattgcc atgcggtgat cgttgtaggt ttgaatgttt gcaggttgca ggttgaaggt
    18841 tgggtggatg gtcataccgt cttcatgctc ttcgacttgc acacctaaac gggcaagttc
    18901 ggtacaggtt gcgtggacac ggtctgtttc cttgacacga gccgaggcga tgccgcgaat
    18961 tcgagtggga gaatc
//
LOCUS       DAOQJC010000075        18894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1344_length_18894_cov_10.623653, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000075 DAOQJC010000000
VERSION     DAOQJC010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18894
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1344_length_18894_cov_10.623653"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1085
                     /locus_tag="PLE39_12050"
     CDS             90..1085
                     /locus_tag="PLE39_12050"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNE69108.1"
                     /translation="MKKQFLRALSILAATSLLLAACGSATPTRTETTGAEPITAEEPL
                     SGTISISGAFALYPMMTVWAEEFTKLHPDVEFDVQGGGAGKGMTDTIAGAVDIGMISR
                     SIKEEETAQGIFWVSVTKDAVFPIINTENPVAAQILEKGITQEIFNKIYITEEIKTWG
                     EVVGDPTITDEIHVFTRSDASGAADQWAKYTGGEAQEDLKGVGINGEPAMLDTVIKDP
                     LGIGFGNLNSIFDLSSGDLVPGIIIPPIDIDNNGKADAVEIYKVKEDAFGAVANGTYP
                     SPPARFENLATLGKPEGLTLAFIEWILTDGQQYLEAAGFVPLTPEQQAESLAKLK"
     gene            1082..2068
                     /gene="pstC"
                     /locus_tag="PLE39_12055"
     CDS             1082..2068
                     /gene="pstC"
                     /locus_tag="PLE39_12055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNE69109.1"
                     /translation="MSQFHIQINDQTEGRQYRPSAALRARQDQNAKRAFFGITLLPVI
                     LIFIVTIALFVRAWPIMSAYPLSDLLFGKVWKPNNGLFGFWPFILGTVWVTSVGVILS
                     VPACILVAMYLAEYAHNTTRSIAKPILDLLAAIPPVVYGVWGLLAVVPFVDDVLAPLA
                     DRWFASVPIFSVTQPTGFGILSGGIVIAVMIAPLIISVLYEIISTVPNDLRHASLAVG
                     ATQWQTIRRIVLPQVTPGIIAAVVLGASRALGETIAVLMVVGNIPQVPTSVFDSAYPL
                     PALIANNYGEMMSIPLYDAALLGAALVLLVVILIFNILSTIVPQRMLRRKWA"
     gene            2110..2928
                     /gene="pstA"
                     /locus_tag="PLE39_12060"
     CDS             2110..2928
                     /gene="pstA"
                     /locus_tag="PLE39_12060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNE69110.1"
                     /translation="MKTIMRIALAIVTGALGLILWAVISRGLPALSWEMVTQVPKGGY
                     YMGKEGGILNAIIGSLYLAIGGTFLAILLCLPIALYLKTYLGDSKWGGYVRLSLDVLW
                     GIPSIVYGAFGFTLMIAFGMRASLLAGIIILALIELPIMARAMDEIIRRMPADLEHAA
                     LALGSTKFEVALQVVTRQMLPGIVTAVLLAFGRGIGDAASVLFTAGYTDRIVTSLMRP
                     TASLPLAVFFQLGSPYPEVQQRGYAAALILTILVLAVSLGSRWLSARLNRYTIK"
     gene            2947..3702
                     /locus_tag="PLE39_12065"
     CDS             2947..3702
                     /locus_tag="PLE39_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNE69111.1"
                     /translation="MDAHIQVKDLNAFYGRQQALKNINIEIPKKQITTIMGPSGCGKT
                     TLLKTFNRFFELTENAQITGQVLVDGENIYDKDVDVTEVRKVVGLLAQRPTPLPMSIY
                     DNIVYGMHIHKMKNDRREYKAAVRHYLEIAGLWEEVQKRLHDPATSLSIGQQQRLCLA
                     RGLAVEPEIILGDEPTSALDPISSQNIESRLIELKQQYTTVIVTHTLRQAKRLADYVI
                     YMYLGEVIEAGPAKEVFSNPKHERTRQYLEGQI"
     gene            4007..5983
                     /locus_tag="PLE39_12070"
     CDS             4007..5983
                     /locus_tag="PLE39_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNE69112.1"
                     /translation="MSISENNQKEIMHRESDYKPPRSLSHWMIGRPLSTADAPHQTIG
                     KAVGLAVFASDALSSTAYATQEILGVLAAAGTIAFGYVFPISIAIVVLLAIVTISYQQ
                     TIHAYPGGGGAYIVARDNLGELPAQIAGAALLTDYILTVAVSISSGVAQIVSAYPNLE
                     DYRVQIAVGAVLLIMLVNLRGVKESGVAFAVPTYFFILMMFTTVGVGLFRSFSGTLGS
                     VINPPELDIMHSVSGISAFLILHAFANGTTALTGVEAISNGITAFNEPRSRNAGITLI
                     WMSLILGALFLGISHLTGQIGAVYSEEETIISQLARTVFNGRGLLYLSAIWGTTVILI
                     MAANTAFADFPRLGALAASDGFLPRQLTFRGSRLVYSNGIVTLSVIASLLIIAFKANV
                     TLLIPLYAIGVFMSFSLSQFGMALRWRKIGRLKEGEEIRERGSVLHYDSKWKSKMLIN
                     GFGALCTLIVMFVFAVTKFSEGAWFVLILTPILVIVFFAIHRHYSSLAHSLSLEKFDG
                     LPARQTRHRVILPISGVHQGAIEALRYARLLSDDVTAVHISLDPAETEKVQKKWITWG
                     EGTRLVILDSPYRLFVEPLLEYLKDIIKNRQPNETITVVVPQFMPSKRWHNLLHMQTA
                     NVLRSELLNQHGVVVTDVPYHVHEKHTEHQEVIK"
     gene            5980..6642
                     /locus_tag="PLE39_12075"
     CDS             5980..6642
                     /locus_tag="PLE39_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNE69113.1"
                     /translation="MNLIVVGCGRVGAELAYRLFKGGHQVTIVDSNRQAFNRLHPDFR
                     GRTLEGEGLAENVLDRAGIKEADGLAAVTNSDTLNAVVAHTARIYYHVPVVVARNYDP
                     NLRPVIESFGLQTVSSTFWGAQRVEELLINPAQRVVYSAGNGEVEVYEVSIPESWNGR
                     TLKELLDPLKNCFAVAVTRAGRSSLPDLGMVLQTGDMLNFSSTYDGIGALTSRLSGKA
                     EA"
     gene            6644..7342
                     /locus_tag="PLE39_12080"
     CDS             6644..7342
                     /locus_tag="PLE39_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNE69114.1"
                     /translation="MFVLITGGGRTGARLANLLLNEKYKVRLVENRRNLLSLLHQELP
                     TEVIYEGNPSDPSVLEAAGIREAHAVASVTGDDSMNLAISFIAKQMFGVPRTIARVNN
                     PNNAWLFDEKFHVDVSLNQADVLAHLIQEEMSLGDMMTLFKIRRGRYAVVEEKVPAGA
                     KAIGVQLREMGLAEHCVIAAIIRDGVMTLPRGDSTLKEFDEIIAVASPEGAKRLAELL
                     ADPAHPDRNDGRKA"
     gene            7491..9293
                     /locus_tag="PLE39_12085"
     CDS             7491..9293
                     /locus_tag="PLE39_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HNE69115.1"
                     /translation="MAKEFHYTWVWELESSPEALWPLASDTNRFNRDTGLPPMELLGI
                     SNGAKLVRFKVPLLDIRWEEDPFEWTYPYRFGILRRYKSGPLAEMRVDCRLEPRQPSG
                     TRIVYDVVVKAANILGDIGIPLAIGKVSAKRFEDTFKRYDRISANIGSIVMNIKGRNL
                     SSAGRTKFKVLSDRLKSQGVDTVILDHLFEYMNNADDLSIQRMRPYALADGWKVNRRT
                     VLETFLRATRVGLLDMYWDILCPECRGVTDDKAHLGELQSTAHCNTCQIDFTANFDHN
                     VEVIFRPNPSVRPVDATVEFCVGSPQRQPHIAFSLTVPPREELPFSTILNEGRYSLHA
                     SDVPGTQIILASNDGVEQIDFRSRITGWPTGITKLGLNPGIRLINETDSTQTFELVRT
                     AWSDQAATAADVTTLQVFRDLFSSEVLRPGEEISVGATTLMFTDLRNSTRMYREIGDA
                     PAFGRVREHFEILEQAVTAEGGSIVKTMGDAIMAAFRNPASALRAVWTVQKQIAARGE
                     PPLSLKVGIHHGPCIVVNLNDRLDYFGSTVNIAARLPNFSTGGEAIISESIRNDQEVI
                     EFLEKNAPPNSLTRFQGDIRGYDQSMDLWRIQMQ"
     gene            complement(9306..10514)
                     /locus_tag="PLE39_12090"
     CDS             complement(9306..10514)
                     /locus_tag="PLE39_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE69116.1"
                     /translation="MHILIIHQAFASLNEPGGTRHHEFARLLTAHGHQVTVIASPVSY
                     ITGSAFRASVSSEVTEDKVTILRASVYDAHHRSFLHRIVAFFSFMLSSFWVGLGVKNV
                     DLVWGTSPPIFQGVTAWVLARIKGAKFLFEVRDLWPQFAIAVGVLKNPVLILLSEWLE
                     RFLYRRADRVMVNSPGFLEPVTSRGAKRVELIPNGADPSMFDPNNDGMEFRRSNQLMD
                     KFVVLYAGAHGMSNDLEVVLDAAALLVDRNEIQLVLLGDGKEKPALMARAQKMDLTNV
                     TFLPSVPKTEMASALAGADACLAILKPLEEYKTTYPNKVFDYMAAGRPVVLAIDGVIR
                     EVVEAAGCGIFAAPGSADEVANAIRKLAADKKESRIMGLQGRDYLQKNFSRAAIGEKL
                     LGLLEDLRSK"
     gene            complement(10556..10999)
                     /locus_tag="PLE39_12095"
     CDS             complement(10556..10999)
                     /locus_tag="PLE39_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE69117.1"
                     /translation="MRIRSGIILIEENKVALIERHRAELHYFTFPGGGVDEGESPEQA
                     AIREAEEELGIQVKLKQKAAEVQFGGRLQVYFLAEKQSGEFGTGTGEEYGEYDPLHGT
                     YQPLWMLLEDMKSRNVVPRELADLVLRFVKEGWPLEPVIIVEEKR"
     gene            complement(11050..11967)
                     /locus_tag="PLE39_12100"
     CDS             complement(11050..11967)
                     /locus_tag="PLE39_12100"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNE69118.1"
                     /translation="MSHYRSLEEVSIQNAWLTIGVFDGVHRGHQQIIHKLVQQAHAQG
                     QPAVVLTFAPHPAAVLGGRDIKCLTTPEERADLLMAMGVDHVITQRFTRDLSTASAKE
                     YMTQLKQSLGLEHLLIGYDFALGRGREGNASRLSEIGNELGYTVEIINALSDESGVIS
                     STEIRKLVSTGNVTEAAKLLGRPYALSGEVIRGAGRGRTINFPTANVDYVQQKVAPSN
                     GIYACWAYLGTEKFMAATNIGFNPTFTPDRQTPSLEAYLLDFDRDIYGQTLKLEFATR
                     IRDELKFDSVEALIEKIHDDVDRTRNLLS"
     gene            complement(12064..12987)
                     /gene="truB"
                     /locus_tag="PLE39_12105"
     CDS             complement(12064..12987)
                     /gene="truB"
                     /locus_tag="PLE39_12105"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNE69119.1"
                     /translation="MNSQDVKNAISGALVVDKPVGMTSHDVVQAIRNGTGLRRAGHTG
                     TLDPRASGVLVILVGPAVRLSEYVSASDKRYQAIIRLGGSTDTFDAEGKVTPTKDPVN
                     VTETQFEEALKTFIGEIEQTPPPYSAVKVQGRKAYEMARQGEEVDLAPRKITVHHLEV
                     LEWTPPEVVIDVHCSSGTYVRSLANDLGVMLGCGAYLVGLRRTKSGRFSLRDSVPLRK
                     LQEAFTAGNWYQYLIPAAEALGDWPAVELNPDEVEGVRHGHRVKVVGEPKETKVRGVS
                     TQGELVALMELITAEDGSHEWQPKKVFFTSD"
     gene            complement(13081..14037)
                     /locus_tag="PLE39_12110"
     CDS             complement(13081..14037)
                     /locus_tag="PLE39_12110"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNE69120.1"
                     /translation="MDNQISGEIKNRLSAAKEIVIVSHVRPDGDAIGSLLGLGLALRE
                     AGKSVQMVLVDGAPASFKYLEGSQLIVKEPQGNHDTFITVDCADFKRTGKIFENFGQP
                     DINIDHHKTNEKFGKLNLIEADEVATAAILTKHLPVWGLKITKAVAAALLTGIITDTL
                     GFRTSNTTPDALRQAATLMESGVDMPEIYMRALVNKSLPAARYWGAGLSSLESRNGVV
                     WGTLTLEDRKSAGYGGNDDADLINMISAIDSHKVGMVFVEQSDHHVKISWRALESGLD
                     VSQIAKHFKGGGHAAAAGADIEGNLQDVQKEVLAKTFEMLNL"
     gene            complement(14105..14455)
                     /gene="rbfA"
                     /locus_tag="PLE39_12115"
     CDS             complement(14105..14455)
                     /gene="rbfA"
                     /locus_tag="PLE39_12115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNE69121.1"
                     /translation="MPSGIRLQRIADRVRQELSELLIREVNDPRLHQIFVTDVTIDRE
                     LAYADIYVSAIEGAGRSAEILEGLESASGFLRRTLSARVEVRAFPRLRFHWDPTPEHA
                     DHIEKVLAGLREKK"
     gene            complement(14516..16294)
                     /gene="infB"
                     /locus_tag="PLE39_12120"
     CDS             complement(14516..16294)
                     /gene="infB"
                     /locus_tag="PLE39_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNE69122.1"
                     /translation="MSSNGNKLELPASIVIRDLALKIEKSPIDLIKKLMSNGVMATIN
                     QTVDFDTAAIVVGEYGFEAVLETVEEEESKAEVGEVPLWRQMIAGENNAFLKPRPPVV
                     TILGHVDHGKTSLLDAIRSTEVAAGEAGGITQHIGAYQVEKKGRLITFLDTPGHAAFT
                     QMRARGAQGADIVVLVVAADDGVMPQTREAIAHAKAARVPLMVALNKVDKANANPDRV
                     KQQLAELELVPDEWGGNTMVIHVSAKQKQGIDDLLEGILLVADSSEIKANPNGKVIGT
                     VIEAELDKSKGVLATLLVQNGTLEASDIVVAGTAYGKLRAITDYKGKPIKKAGPSTPV
                     AVMGLSGMPSAGDIFEVVDSEKEARNIVNERVEAAKTLSQTRKKVSLEDLFANVQAGE
                     AKGLNLIIKADVQGSLDPIIGELNKLGEGEIGLHILHAETGNISNNDVMLAAASRAII
                     IGFNVQADVDGRRLAEKEGVDIRLYEIIYRMTEDIEKALKGMLEPKLVEKVIGRAQVL
                     AVFSASKFGKVAGCKVTDGELKRGARVRLFRGTDMIYEGDLSSLRHEKEDVKEIRQGY
                     ECGVGFKSFNDILPGDQLVCYIVENS"
     gene            complement(16403..16657)
                     /locus_tag="PLE39_12125"
     CDS             complement(16403..16657)
                     /locus_tag="PLE39_12125"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HNE69123.1"
                     /translation="MGCREVLPKRQMVRIVRTDNGVWVDPTGKAAGRGAYLHDQRECW
                     VRGLRGALGHALKAELTADDRARLEEFMNSLPLSEAEAGK"
     gene            complement(16726..18618)
                     /gene="nusA"
                     /locus_tag="PLE39_12130"
     CDS             complement(16726..18618)
                     /gene="nusA"
                     /locus_tag="PLE39_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNE69124.1"
                     /translation="MAKNEFALAFNEVLEEKQLAKDVVIAAIESAMVSAYRRAVSAST
                     AQHVEAKLDPDTGEVTVYAEKEVVEDSVVDARTEVLLGEAQKVKPDTQVGDMVLVETT
                     PADFGRVAAQTARQVIQQRIREAERSNQMQYFERQVGEIVSGLVQASNAQSATIGLDM
                     KAEGILPNNQKIPGERLKLHDRIRAVVMEVKDGTRGPQIILSRAHRNFLRRLLENEVP
                     EIYHGIVEIRAIAREPGARAKVAVSATQPGIDPVGACVGIKGVRIQAIVKELHDEKID
                     IIQWDQDPITYISKAISPARVNGVYLVDTEGARTATVVVGEDQLSLAIGRDGQNARLA
                     AKLTGWRIDIKSLTEAASDTLDKLQANAELGAMLPAAMEAIPQVQEVLAKKAEGRAIT
                     PEEYTVLGQFIDRVERRTIQIKEEAARVEEERTVAARADIPAAAFQMPLDQAGMKEHI
                     FNILTEANFETVGQLMFVLKTEPNKVLGLPGIGSKAMQNIEESLAVLTFPEPEKVEEA
                     PVAVVAAEPVVVETAPVEPAAAAEETPVVKKQAEGKRDAKRPVEVEEDEEHAKDGVSL
                     DELFKMKPEIFQGARPAEEEVSGDKKKSKKGKKKAVELEFDEGLGTVVGRKIHKRGGD
                     VEGDWE"
BASE COUNT         4431 a         5296 c         4544 g         4623 t
ORIGIN      
        1 ctgcgtgcag gggagcgcat ttttatttac ctcaaaagta tactagtact atagatatta
       61 tgatatttac aatccatagg agaaccctga tgaaaaaaca attcttgaga gcgttatcga
      121 tactggcagc aacctcgctg cttctggctg cttgcggctc cgctacaccg actcgcaccg
      181 aaacaacggg ggcagagccc atcactgctg aagaaccgct cagcggaacc atttccatct
      241 ccggcgcttt cgccctctac cccatgatga ccgtctgggc tgaagaattc acaaagcttc
      301 accctgatgt cgaattcgac gtacaaggcg gcggtgcagg gaaaggcatg accgacacca
      361 tcgcgggcgc agtggatatt ggcatgatct cccgctccat caaggaagag gaaaccgcac
      421 agggaatctt ctgggtctct gtgacaaaag atgcggtctt cccgatcatt aataccgaaa
      481 acccggtggc cgcccaaatt cttgaaaaag gtattacaca ggaaatcttc aacaagatct
      541 atatcactga agagatcaaa acctggggag aagtggtcgg cgaccccacc atcaccgatg
      601 aaatccacgt cttcacccgt tccgatgcat ccggcgctgc agaccagtgg gctaaataca
      661 caggcggcga ggcgcaggaa gacctcaaag gtgtgggcat caatggcgaa cccgccatgt
      721 tggatacggt catcaaagac cctttgggta tcggttttgg aaacctgaac agcatcttcg
      781 acctctccag cggagatctc gtccccggca tcatcattcc cccgatcgat atcgacaaca
      841 acggcaaggc agatgccgtt gaaatttaca aagtcaagga ggatgcattt ggcgcagttg
      901 ccaacggcac atatccatcg ccacccgcac ggttcgaaaa cctcgccacc ctcggcaaac
      961 cggaaggatt gaccctggcc ttcatcgagt ggattttgac agatggccag caataccttg
     1021 aagccgcagg tttcgtaccg ctcaccccgg aacaacaagc cgaatcgctt gcaaaactaa
     1081 aatgagccaa ttccacattc aaatcaatga tcaaacagaa ggccggcaat accggccttc
     1141 tgccgcgctt cgtgcccgtc aagaccagaa tgcaaaacgg gcatttttcg gcatcaccct
     1201 gttgccggtg attctgattt ttattgtcac gattgcgttg ttcgtccgcg cctggccgat
     1261 catgagcgcc tatcccctct cagacctgtt atttggcaaa gtatggaaac ccaataacgg
     1321 tctgtttggc ttctggccct tcatccttgg cacggtctgg gtcacttcag tcggggtcat
     1381 tctttcagta cctgcctgca ttctggttgc catgtacctg gctgaatacg cccacaacac
     1441 cactcgctcc atcgccaaac ccattttaga cctgctggca gccatcccac cggtagtcta
     1501 tggcgtctgg ggattgctgg ccgtggtccc atttgtggat gacgtactcg cgcctcttgc
     1561 cgaccgttgg tttgcatctg ttccgatctt cagtgtcacc caaccaacag ggttcggaat
     1621 cctctcaggc ggaatcgtca tcgcggtcat gattgcgccg ttgatcatct cggtcctcta
     1681 cgagatcata tccaccgtcc ccaatgactt gcggcatgct tcactggcag tgggcgccac
     1741 tcaatggcaa accatccgca gaatcgtcct gccgcaggtg acaccgggca tcatcgcagc
     1801 ggtggtgctt ggcgcatcac gcgccctcgg tgaaaccatc gccgtactga tggtggtcgg
     1861 caatattcca caggtgccca catcagtgtt cgattcggca tacccgctcc cggcgctgat
     1921 cgcaaacaac tacggcgaaa tgatgtccat cccgttgtat gatgcggctt tgctcggggc
     1981 ggcactcgtc ctgcttgtcg tcatcctgat ctttaacatc ctctccacca ttgtgcccca
     2041 aagaatgttg aggcgaaaat gggcataaag aaaaatccat tccaaaggcg tcacatcatt
     2101 gagctattca tgaaaaccat catgcggatt gcgcttgcga tcgttacagg cgcactcggg
     2161 ttgatcttat gggcggtcat ttcacgcggc ctgcctgcac tttcatggga gatggtgaca
     2221 caagttccca aaggcggata ttacatgggc aaggaaggcg gcatcttaaa tgccatcatt
     2281 ggctcgttat atctcgccat tggcggcact tttttggcca tcctactctg cctgccaatt
     2341 gcactttatt tgaaaaccta cctcggcgat tcaaaatggg gcggttacgt ccgcctttcg
     2401 cttgatgtgt tgtggggtat tccctccatt gtatacggcg ctttcggatt cacattgatg
     2461 atcgcctttg ggatgcgcgc ctccctgctg gcaggcatca tcatccttgc cctcatcgaa
     2521 ttacccatca tggcccgcgc catggatgaa atcatccgcc gcatgcccgc agacctggaa
     2581 catgccgcgc tggcgctggg ttccaccaaa tttgaagttg cactccaagt ggtaacccgc
     2641 caaatgctgc ccggcattgt cactgctgtg ctactcgcat tcggacgcgg cattggcgat
     2701 gcagcctctg tcctgttcac ggcaggttat acagaccgca ttgtcacttc tttgatgcgc
     2761 ccgaccgcat ccctgcccct cgctgttttc ttccagcttg gctcgcccta ccctgaagtg
     2821 cagcagcgcg gatatgccgc cgcattgatc ctgaccattc tcgtgcttgc agtcagcctc
     2881 ggttcacgct ggctctcggc acggttgaac agatacacca tcaagtaata tacaaaagga
     2941 ttttatatgg acgcccatat tcaagtaaaa gatctcaacg ctttttacgg caggcaacaa
     3001 gccctgaaaa atatcaacat tgaaatcccc aagaaacaga tcaccacaat catggggcct
     3061 tccggctgcg gcaaaaccac cctgctcaaa accttcaacc gcttctttga gttgaccgaa
     3121 aacgcccaga tcacaggtca ggtgcttgtg gatggcgaaa acatctacga caaggatgtg
     3181 gatgttaccg aggtgcgcaa ggtggtcggg ctgctggcgc agcgtcccac accattgccc
     3241 atgtcaattt acgataacat cgtgtatgga atgcatatcc acaaaatgaa gaacgaccgc
     3301 agggaataca aggcggctgt gaggcattac cttgagattg ccggtctgtg ggaggaagta
     3361 caaaaaaggc tgcacgaccc cgccaccagt ctctccattg gacagcaaca acgcctctgc
     3421 ctcgcccgcg gcctggccgt tgaacccgag atcatcctcg gcgacgaacc cacttcggcc
     3481 ctggacccga tctcatcgca aaatatcgaa agccgcttga tcgagttgaa acaacaatat
     3541 acaacggtca ttgtcactca cacactgcgg caggccaaac gccttgctga ctatgtcatt
     3601 tacatgtatc tcggcgaagt catcgaagcc ggcccggcca aagaagtctt ttccaaccca
     3661 aaacatgaac gaacccgtca ataccttgaa gggcaaatct aaatctttat ttccaataga
     3721 agcagttggt tcgcatcagc ctgtccatgc tgtatggaca ggctgattgt gttttagaca
     3781 gcagacactc tctgaaaaca tggaatcgga ctataatcac gccttgttcc atttctcaag
     3841 catttatcct ctggcaatat gagcacccca aaagaaactt cccccaacct ttttgacagc
     3901 taatcaattg tgcgatcatt tcaaccaatc cctgcgcagt gaaacttgtt tcgaaattca
     3961 gcgtaaaggg tgatgggttg tgtgtttttt tatagaaaga gagaaaatgt ccatttcaga
     4021 aaacaaccaa aaagaaatca tgcatcggga aagtgattac aagccgccca gatcattaag
     4081 tcactggatg atcggcaggc cgctttccac agccgatgcc ccgcatcaga cgatcggaaa
     4141 agccgttggt ctggctgtct ttgcatctga tgcgctctcc tccactgctt acgccacaca
     4201 ggaaattctg ggagttttgg ccgcggcagg cacgatcgca tttggatatg tttttccgat
     4261 ttccattgcc atcgttgttc tgctggcgat cgtgaccatt tcataccagc agaccatcca
     4321 tgcctatccg ggcggcggtg gtgcgtatat tgtggcccgc gacaatttgg gagaattgcc
     4381 cgcacagatc gctggtgccg ccctgttgac agattacatc ctcacagtcg cggtgtccat
     4441 ttcatcgggt gtggcgcaga tcgtatccgc ctacccaaac ttggaagatt accgtgttca
     4501 gatcgctgtc ggggcggtgc tgttgatcat gctggtcaat ttgcggggtg tcaaggaatc
     4561 gggcgtggct ttcgcagtcc caacctattt cttcatcctc atgatgttca ccaccgtggg
     4621 tgtgggtctg ttccgaagtt tctccggtac gctgggaagt gtgatcaacc cgcctgagtt
     4681 ggacatcatg cacagcgttt cgggcatctc cgcatttttg atcctgcatg cttttgcaaa
     4741 tggcaccaca gccttgaccg gtgtggaagc catctccaat ggtattactg cgttcaatga
     4801 accgcgcagc cgcaatgcag gcatcaccct gatctggatg tcgctcattt tgggtgcttt
     4861 gttcctcggc atttcacatc tgaccgggca gatcggggca gtgtactctg aagaagagac
     4921 catcatttca caattggcgc gtacggtatt taatggccgc gggctgctct atctttccgc
     4981 catctggggt acgaccgtca tcctcatcat ggcagccaat acagcttttg ccgattttcc
     5041 acgcctcggc gcgctggcgg ccagtgacgg cttccttccc cgtcagttga cctttcgcgg
     5101 cagccgcctt gtgtattcga acggtattgt gactttatct gtcattgctt ctcttttgat
     5161 cattgcattc aaagccaacg tgacgctctt gatcccgttg tatgccatcg gtgtgttcat
     5221 gtctttctcg ttatctcaat ttggcatggc acttcgctgg cgcaagatcg gacgacttaa
     5281 agaaggcgag gagatccgcg agcgcggttc ggtgctgcac tatgacagca agtggaaatc
     5341 caagatgctg atcaatggat tcggcgcact gtgtaccctg atcgtgatgt ttgtttttgc
     5401 tgttacgaaa ttcagtgaag gtgcatggtt cgtgttgatc ctgacgccga tccttgtgat
     5461 cgtcttcttc gcaattcacc gccattattc cagcctggcg cacagccttt cccttgaaaa
     5521 gttcgacgga ctgccagcac gtcaaacccg tcaccgcgtc atcctgccca tcagcggcgt
     5581 gcatcagggt gccattgaag ccttgcgtta tgcccgtctg ctttcagatg atgtgaccgc
     5641 cgtccacatt tcactggacc ctgccgagac ggaaaaggtt caaaagaaat ggataacctg
     5701 gggtgaaggc acacgcctcg ttattctgga ttctccctac cgactcttcg ttgagccgct
     5761 gcttgaatac ctgaaagaca tcatcaagaa ccgccaaccc aacgaaacca tcacggtagt
     5821 agtgccgcag ttcatgcctt caaagcgctg gcataacctg ttgcatatgc aaactgccaa
     5881 cgttttgcgc agtgaattat taaaccaaca cggggtggtc gtcaccgacg taccctatca
     5941 tgtgcatgaa aagcatacag aacaccagga ggtgatcaaa tgaacttaat tgttgttgga
     6001 tgcggccgcg taggcgctga actggcctac cgccttttca aaggcggcca tcaagttacc
     6061 atagtagata gcaatcgaca ggctttcaac cgcctgcacc ctgattttcg cggacgaact
     6121 ctcgaaggtg aagggttggc tgaaaacgta cttgaccgtg cagggatcaa agaggcagac
     6181 ggccttgcgg ctgttaccaa ttcagatacc ttgaacgcgg tggtggccca taccgcacgc
     6241 atttactacc atgtccctgt ggtggtggcg cgtaattacg accccaatct gcgcccggtc
     6301 attgaatcct ttggcctgca aacggtcagc tccacatttt ggggcgcaca gcgcgtggag
     6361 gagttattga tcaaccctgc ccaacgcgtg gtgtattcgg cgggcaacgg cgaagtggaa
     6421 gtatatgagg tgagcattcc cgaatcgtgg aatggacgca cgctcaagga gttattggat
     6481 ccgcttaaga actgctttgc agtggcggtt acacgggccg ggcgctcgtc cctgccagac
     6541 ctgggaatgg tcctgcaaac tggagacatg ctcaacttca gcagcaccta tgatggcatc
     6601 ggcgcgctca cttcacgctt gagcggaaag gcggaggctt aacatgttcg tattgatcac
     6661 cggaggcggc cgtaccggag cccgccttgc caacctgtta ctaaacgaaa aatacaaggt
     6721 gcgtctggtg gaaaaccgcc gcaacctgct ctctctctta caccaggaac ttccaactga
     6781 ggtgatttac gaaggcaacc catctgaccc gagcgttctc gaagcagcag ggattcgtga
     6841 agcacacgcc gtggcttccg tgaccggcga tgactcgatg aacctcgcca tcagtttcat
     6901 tgccaagcaa atgtttggcg tgcctcgcac cattgcccgc gtgaacaacc cgaacaatgc
     6961 ctggttgttc gatgaaaaat tccatgtgga tgtttccctc aatcaggctg atgtacttgc
     7021 acacttgatc caggaggaaa tgtcactggg tgacatgatg acccttttca agatccgtcg
     7081 cggacgttac gccgtggtgg aagaaaaggt ccccgctgga gcaaaagcca ttggcgtcca
     7141 acttcgggag atggggctcg ccgaacactg cgtgatcgca gccatcatcc gtgatggggt
     7201 catgaccctg ccgcgcggcg acagcaccct gaaagagttc gatgagatca ttgcggtagc
     7261 cagcccggaa ggcgccaaac gactggccga actgttggca gaccctgccc accctgaccg
     7321 aaacgacggt agaaaagcct aaccccctgc ctacatcaat cagattctaa aaacaaggcc
     7381 tgcccgaaac caaaaattgg ggaggccttg tttttttatc acaaccgcgc tgcaatgctt
     7441 atgagaggaa tcttgagtaa aataatcatg attatgattg tctaaccaac atggcaaaag
     7501 aattccacta cacatgggtt tgggagcttg agtcctcacc ggaggcgctc tggccgcttg
     7561 cctcagatac gaatcgcttc aaccgcgata ccggcctgcc ccccatggaa ttgctcggaa
     7621 tttcgaacgg cgcaaagctt gtccgtttta aagtgccctt gttggatatt cgttgggagg
     7681 aagatccctt cgagtggacc tatccctatc ggtttggaat tctacgccgc tataaaagcg
     7741 gcccgctcgc agagatgcgt gtggattgcc gcctcgaacc aaggcagcct tcgggcacac
     7801 gcattgttta tgacgtggtt gtcaaagcag caaacattct tggagacatt ggcattccgc
     7861 ttgcgatcgg caaggtcagc gccaaacgtt ttgaagatac cttcaaacgg tacgaccgta
     7921 tttcagccaa catcggttcg attgtgatga atatcaaagg gcgaaatctc tcatcggctg
     7981 gccgtaccaa gttcaaggtt ttgagcgacc gcttgaaaag tcagggcgtg gataccgtca
     8041 tcctcgatca tctttttgaa tacatgaaca atgccgacga tctttccatc cagcgcatga
     8101 ggccttacgc actggctgat ggctggaagg taaaccgccg cactgtgttg gaaacctttt
     8161 tgcgcgccac ccgcgttggg ctgctcgaca tgtactggga tattctttgc cccgaatgcc
     8221 gcggcgttac cgacgacaaa gcccacctcg gtgaacttca atccacagcg cattgcaaca
     8281 cctgccagat cgatttcacc gccaacttcg accacaacgt agaagtcatc tttcgcccca
     8341 acccctcggt gcggcctgtg gatgccactg tggaattttg tgtaggcagt ccgcagcgcc
     8401 aaccgcatat tgccttttcg ctcaccgtac cgccgcgaga agaacttcct ttttcaacca
     8461 tcctcaacga aggccgctac tccctgcatg cgtcagatgt tcccggcacg cagatcatcc
     8521 ttgcttcgaa cgatggtgtg gaacaaatcg attttcgctc gaggattacc ggctggccga
     8581 ccggcatcac caaacttgga ctcaaccctg gcatccgtct catcaacgaa accgattcaa
     8641 cccagacctt tgagctggtg cgcacagcct ggtcagatca ggcggcgacg gctgccgacg
     8701 tgacgacatt acaggttttc cgcgaccttt tttcaagcga agtgctgcgc cccggtgaag
     8761 agatttccgt cggcgccacc accttgatgt tcaccgacct ccgcaattca acacggatgt
     8821 atcgtgagat cggcgatgcc ccggcctttg ggcgtgtgcg cgaacatttt gagatcctcg
     8881 aacaagccgt taccgctgaa ggcggttcca ttgtcaaaac catgggagat gccatcatgg
     8941 cagcattccg taaccccgct tcggccctgc gcgccgtgtg gaccgtgcaa aaacaaattg
     9001 ccgccagggg tgaaccgccg ttgtccctca aagttggcat tcatcatgga ccctgcatcg
     9061 tcgtcaacct gaacgatcgg ctcgactact ttggctccac tgtaaatatc gctgcgcgcc
     9121 tgccaaattt ctccacaggc ggcgaggcga tcatttccga atcgatccgc aacgatcaag
     9181 aagtgatcga attccttgaa aagaacgcac cgcccaattc tctcacccgt tttcagggag
     9241 acattcgcgg ctacgatcaa tccatggatc tatggaggat tcaaatgcaa taaaggggtg
     9301 gaaatctatt tcgatctcag gtcttccaac aaaccaagca atttctcgcc aatcgcggcg
     9361 cgactgaaat tcttttgcaa ataatcccgt ccctgcaagc ccatgatacg gctttctttt
     9421 ttatcagcgg ccagtttacg gatcgcattt gcaacttcgt ccgcgctgcc cggcgcagcg
     9481 aatattccac acccggcggc ctcaaccact tcgcggatca caccatcaat tgcaagaaca
     9541 acagggcggc cagccgccat gtaatcaaaa accttgttcg ggtaggtggt cttatattct
     9601 tcaagcggtt tgagaatggc aaggcatgca tctgcccccg caagcgcaga cgccatttct
     9661 gttttgggta cggaaggcaa aaaggttaca ttcgttagat ccattttttg tgcgcgtgcc
     9721 atcaatgctg gcttttcctt gccatcaccc agcaaaacaa gttggatctc atttcgatcc
     9781 accagcaagg cagctgcatc caacacaacc tcaagatcgt tcgacatgcc atgcgcaccc
     9841 gcatacaaca caacaaattt atccatcaat tgattcgaac gacggaattc catcccatcg
     9901 ttgtttggat cgaacatgga agggtctgcg ccgttgggga tcaattccac ccgcttcgcg
     9961 ccccgactgg taactggttc taaaaaaccc gggctgttga ccatcacccg gtcagcgcgg
    10021 cgatagagaa aacgctccag ccattcagaa agcagaatca aaacagggtt tttcaataca
    10081 cccacggcaa ttgcaaattg cggccacagg tcacgcactt caaataaaaa tttcgcaccc
    10141 ttgatccgcg ccaagaccca tgcagttacc ccttgaaaaa tgggtggtga cgtaccccaa
    10201 acaaggtcta cgttttttac gccaagtccc acccaaaacg aagagagcat aaaagagaaa
    10261 aaggccacaa tgcggtgcag aaaggatcta tgatgcgcat cgtacacact ggcacgaagg
    10321 atggtgacct tatcttccgt gacttctgaa gaaacggaag ctcgaaacgc agaaccggtg
    10381 atgtaactga ccggtgaagc gatcacggtc acctgatgac catgtgccgt cagcaggcgc
    10441 gcaaactcat ggtggcgcgt gccgccaggt tcattgagag atgcgaatgc ctgatgaatg
    10501 ataaggatat gcatttaacg aaatggatca aatttcaatg atacgccgca aactatcagc
    10561 gcttttcttc gacgattata actggctcta atggccagcc ttcttttaca aaacgcaaaa
    10621 ccagatcagc cagttcacgc ggaacaacgt tcctgctctt catatcttcc agaagcatcc
    10681 ataagggttg gtaggtaccg tggagcgggt catattcacc gtattcttca ccggtgccgg
    10741 tgccgaattc cccgctttgc ttttcagcta aaaaatacac ctgcagcctc ccgccaaact
    10801 gcacttccgc cgccttttgt ttcagcttca cttgaatccc aagttcctct tccgcctctc
    10861 gaatggcggc ttgttcgggg ctttcgcctt catccacgcc gccaccggga aaggtgaaat
    10921 aatgcaattc cgcccggtga cgttcgatca gggcgacttt gttctcttcg atcaggatga
    10981 ttccggaacg tatgcgcatg gtatgcctcc cgttgattct ccaaagctca tggctttggc
    11041 cctcccagtt taagacaaca gatttctggt ccgatccaca tcatcatgga tcttttcgat
    11101 gagcgcttcc acagagtcaa atttcaactc gtcgcggatt cgtgttgcga attcaagttt
    11161 caaggtctgg ccgtaaatat cgcggtcgaa atcgagcagg taagcttcaa ggctgggcgt
    11221 ctgccgatcg ggtgtgaagg ttgggttgaa gccaatgttg gtggcagcca tgaatttttc
    11281 tgtgccaagg tatgcccagc aggcgtaaat gccattggat ggggcaacct tttgctgcac
    11341 ataatctaca ttggcggtgg gaaagttgat ggtacgtccg cgcccggcgc cgcggatgac
    11401 ctctccgctg agggcatagg gacggccaag caattttgcg gcttcggtaa cattccccgt
    11461 ggaaacgagt tttcgaatct ctgttgaaga gatcactccg ctttcatcgc tgagcgcgtt
    11521 gataatttcc actgtgtatc ccaactcatt gccgatctcg ctcaggcgtg aggcatttcc
    11581 ttcgcgcccc ctgccaaggg caaagtcata accgatgagc aggtgttcga ggccaagact
    11641 ctgcttcagt tgagtcatat attctttggc agaggcagtt gaaaggtccc gtgtgaagcg
    11701 ttgagtaatg acatgatcca cacccatcgc catcaaaaga tcagcgcgtt cttcgggtgt
    11761 ggtcaggcat ttaatgtcac gcccgccgag tactgcggca gggtgcgggg caaaggtcag
    11821 cacaacagcg ggctgacctt gtgcatgggc ttgctgtacc agtttgtgga tgatctgctg
    11881 gtgtccgcga tgcacgccat cgaacacacc aatggtgagc catgcgtttt gtatggatac
    11941 ttcttcaagg gagcgataat gagacatgat ataaaaaagc cgccggggcg gcggctggtg
    12001 tcattgcaag ggtattccat ttctgtgttg aatgaaatac cctcttcgca atgaccctat
    12061 ttattaatcg gaagtaaaga aaaccttttt gggctgccat tcgtgcgagc catcttcggc
    12121 tgtgatcagt tccatcagag caaccaactc accctgtgta ctgacaccgc gaaccttcgt
    12181 ttccttgggt tcaccgacaa ctttgacacg gtgcccatgg cggacgcctt caacttcatc
    12241 gggattaagc tccacggcag gccaatcacc aagcgcttct gcggcgggga tcagatattg
    12301 ataccagttt ccggcggtga aggcttcctg taatttgcgc aggggcacgg aatcgcgcag
    12361 ggagaaacgt ccgctcttgg tgcgacgcag gccaacaagg taggcgccgc atccgagcat
    12421 gactccaaga tcattggcca gtgagcggac ataggtgccg gaagagcaat gcacatcaat
    12481 caccacttcg gggggagtcc attcaagcac ttcgagatga tggacggtga tcttacgcgg
    12541 ggccagatca acttcttcac cctgacgcgc catttcatag gctttgcgtc cctgcacttt
    12601 tacagcagag tagggtggcg gggtttgttc gatctcacca ataaatgttt tcagggcttc
    12661 ttcaaattgt gtttcggtaa cattgaccgg gtcttttgta ggtgtgacct tgccttcagc
    12721 atcgaaggtg tcggtggaac cgcccagacg aatgatggct tgataacgtt tatcagaagc
    12781 agatacatat tcgctcaaac gcacagccgg gccaacaaga atgaccagta cgccggaagc
    12841 acgtggatcc aatgtgcccg tatgacctgc acgacgcaga ccagttccat tacggatcgc
    12901 ctgcaccaca tcatgagagg tcataccaac aggcttatct acaacaagag cgccagatat
    12961 ggcatttttt acatcctgac tattcatctt gcattcctcc ttctaatatt ctccagttaa
    13021 ttatgacaca tttgtccaat tttacaacga ttactgtggt catggagttt tttattccac
    13081 ttacagattt aacatttcaa aggttttcgc aagaacttcc ttttgaacat cctgcaaatt
    13141 accttcgatg tctgcacctg ctgcagcggc atgcccacca cctttgaagt gtttggcgat
    13201 ctgcgataca tcaagacctg attccagtgc gcgccatgaa attttcacat ggtgatcgct
    13261 ttgctctaca aaaaccattc ccactttgtg agaatcgatt gcagagatca tattgatcag
    13321 gtcggcatca tcattgccgc catatccggc tgatttacga tcttcaaggg tcaatgtgcc
    13381 ccacactaca ccatttctgc tttccaaact ggagagaccg gcaccccaat agcgtgcggc
    13441 aggcagggac ttgttcacca gagcacgcat gtaaatctcg ggcatatcca cgccggattc
    13501 catcaaggtg gctgcctgtc ggagggcatc cggggtggta ttggaggtgc ggaacccaag
    13561 ggtatcggtg atgatgccgg tcagcaaagc agcggctacc gctttggtga tcttcaaacc
    13621 ccacacaggc aaatgcttgg tgaggatggc ggcagtggca acttcatccg cttcgatcag
    13681 gttgagcttt ccaaattttt cgttggtctt gtgatggtca atattgatat cgggctggcc
    13741 gaaattctca aagatcttgc cagtacgttt gaaatcggcg caatccactg tgatgaaggt
    13801 gtcatggttc ccctgaggtt ccttcacgat caactggctt ccttccaggt atttgaatga
    13861 ggcaggcgcg ccatcaacaa ggaccatttg cacggatttc cccgcttcac gcagagccaa
    13921 tcccagtcca agcagcgagc caattgcatc cccatccggt cgaacgtgcg acacgatcac
    13981 gatctctttt gccgcactta atctattttt tatctcccct gaaatttgat tgtccactta
    14041 tgcttatcct ccaggcactt aaaaaataag gggtgcattt gaacgcaccc cttgtaaatt
    14101 acatttattt cttctctctt aaccctgcca gaactttttc gatatggtct gcgtgttccg
    14161 gagttgggtc ccaatgaaac cgcaaccttg ggaaagcccg cacttccaca cgggctgaaa
    14221 gggtgcgcct gagaaaacca gaggcagatt ccagaccttc aagtatctca gccgagcgcc
    14281 ctgcgccctc aatggccgat acataaatat cggcataagc cagttcccgg tcgatcgtca
    14341 catcggttac gaagatctgg tgtaaacgcg ggtcattaac ttcacgaatt agcagttccg
    14401 acaattcctg ccggacacga tctgcaatac gctgtaaacg aattcctgat ggcataaagc
    14461 gcctcacatt catcgctgca ggcgtgttta acgtccatac aacgaatcac atatattacg
    14521 aattttcaac aatataacaa acaagctggt cgccgggcag aatatcatta aagctcttga
    14581 aaccgacgcc gcattcataa ccctgacgga tctctttcac atcttccttt tcatggcgga
    14641 gcgaagagag atcgccttca tagatcatat cagtaccgcg gaagaggcgg acacgcgcgc
    14701 cgcgtttgag ttcaccatct gtcaccttgc agcctgccac cttgccgaac ttcgatgcag
    14761 aaaataccgc cagaacctgt gcccgcccaa tgaccttttc aaccagtttc ggttcgagca
    14821 tgcctttgag agccttctca atatcttcgg tcatgcggta aatgatctcg tacaggcgga
    14881 tatccacgcc ttctttttcg gccaagcggc ggccatccac atcggcttgt acattgaaac
    14941 caatgatgat ggctcgcgaa gccgccgcaa gcataacatc gttattgctg atattaccgg
    15001 tttccgcatg caggatgtga agaccgatct caccttcccc gagcttgttc aactcgccga
    15061 tgatcggatc cagcgaccct tgcacgtcgg ctttgataat gaggttgagt cccttggctt
    15121 cacctgcctg aacattggca aacaggtctt cgagtgaaac ctttttcctg gtttgtgaaa
    15181 gggtttttgc ggcttccaca cgctcgttca caatattgcg ggcttctttt tcagaatcta
    15241 caacttcaaa tatatcacca gcggaaggca tgccgcttaa acccatcaca gccaccggcg
    15301 tagatggacc ggctttcttg atcggcttgc ccttgtaatc ggtaatggcg cgcaattttc
    15361 cgtaggcggt ccctgctaca acgatgtcac tagcttcaag ggtcccgttt tgaacgagca
    15421 atgtcgccag cacacctttg cttttatcaa gctcggcttc aatgactgtg ccgataactt
    15481 ttccattggg gttggcttta atctcgctgc tatctgctac aagcaggatg ccttcgagca
    15541 aatcatcaat accctgtttc tgttttgccg aaacatggat caccatggta ttcccgcccc
    15601 attcatccgg gacaagttca agttcagcaa gttgttgttt aacacggtca ggattggcgt
    15661 tcgctttatc caccttgttc aatgcaacca tcaacggcac ccgcgcagcc ttggcatggg
    15721 caatggcttc acgggtctgt ggcatcacac catcatccgc cgcaacgacc aacacaacaa
    15781 tgtccgcacc ctgggcgccg cgggcacgca tttgggtaaa tgcggcatgg cccggtgtat
    15841 ccaggaaggt gatcaaacgt cccttctttt caacttgata tgcaccgata tgctgtgtaa
    15901 tgccgcctgc ttcgcctgcc gccacttcgg ttgaccgaat ggcatccaac aaactggtct
    15961 ttccgtggtc aacatgccca agaatggtta caacaggcgg gcggggcttc aaaaatgcat
    16021 tgttttcccc ggcaatcatc tgacgccaga gaggcacttc ccccacttcc gctttgcttt
    16081 cctcttcttc aactgtttcg agaaccgctt caaacccgta ttcaccaacc acgatcgcag
    16141 ccgtatcaaa atccacggtt tgattaatgg ttgccatcac gccatttgac attaattttt
    16201 tgatcagatc aatcgggctt ttctcgatct tcagcgccag gtcacgaatc acaatgctgg
    16261 caggtaactc aagtttattt ccgttgctgc tcataggcca cctccatcta actagtatgc
    16321 ggattacaac ctacagaatc cgcgaaattc tcaatttacc agcgagaggc ccgtgagcga
    16381 gtggctattc gttcacatga ctttacttgc cggcctctgc ctcagaaaga ggcagagagt
    16441 tcataaattc ttccagtcga gcgcggtcat ccgcagtcaa ctcggctttg agcgcgtgtc
    16501 ccaaagcccc ccttagcccc cgcacccaac attcacgttg atcgtgcagg tatgctcccc
    16561 tcccggcggc tttgccggtc gggtcaaccc aaaccccatt gtccgtacgt acgatgcgca
    16621 ccatctgcct ttttggcaaa acttcgcgac aacccacgca agtccgctgg gggatatgct
    16681 ttacacgttg ggcaggtttt ttggccacct tcaagatacc tgaaactatt cccaatcacc
    16741 ttcaacgtcg ccgccgcgtt tgtgaatctt acggccgact acggttccca aaccttcatc
    16801 gaactccaat tcaacggctt tcttcttgcc cttcttgctc ttcttcttat cgccgctgac
    16861 ttcctcttcg gcagggcgcg caccctggaa gatctcgggc ttcatcttga acaactcatc
    16921 aagagacacg ccatccttcg catgctcttc atcttcttca acttcaactg gacgcttcgc
    16981 atcgcgcttc ccttcggcct gcttcttcac aaccggcgtt tcctccgccg ctgcggcagg
    17041 ttctacagga gcagtctcga caacgacagg ctcggcagcc acaacagcaa caggagcctc
    17101 ttccaccttt tcaggttcgg ggaaggtcaa tacagccagc gactcttcaa tgttttgcat
    17161 ggccttcgag ccaatacccg gcaaaccaag caccttgttg ggttcagtct tgagaacaaa
    17221 catcaactga ccaactgttt caaaattggc ttcggtcaaa atattgaaaa tatgctcttt
    17281 catgccagcc tggtcgagcg gcatctggaa ggcggcggca gggatatccg cgcgggcggc
    17341 tacagtgcgt tcctcttcca cacgggcagc ttcttccttg atctggatcg tgcgtcgttc
    17401 tacgcggtca atgaattgac ccagtaccgt atattcttcc ggagtaattg cacggccttc
    17461 ggccttcttg gcaaggactt cctgcacttg aggaatggct tccatggcgg caggcaacat
    17521 tgcgccaagc tcggcattag cctgcaattt atcgagggta tcactggcag cctcggtcaa
    17581 agacttgatg tcaatacgcc agccggtcaa ttttgcagcc aggcgggcat tctgaccatc
    17641 acggccgatg gcaaggctta actggtcttc acccaccacc acagttgcag tgcgggcgcc
    17701 ttcagtatcc acaaggtaca cgccatttac gcgtgccgga ctgatggctt tggaaatata
    17761 ggtaatggga tcctgatccc attggatgat atcgatcttc tcatcgtgca attccttgac
    17821 gatggcctga atacgcacac ccttgatgcc cacacatgca cccaccgggt caataccggg
    17881 ttgtgtggca gaaaccgcca cctttgcacg cgcacctggt tcacgcgcaa tggcgcggat
    17941 ctcaacaatg ccatgataaa tttcaggaac ttcattttca agcaagcggc gcaggaaatt
    18001 acgatgcgca cgagacagaa tgatctgtgg tccgcgtgtg ccatccttga cttccataac
    18061 aaccgcgcgg atacggtcat gcagtttgag gcgttccccc ggaatcttct ggttattggg
    18121 cagaatacct tcggccttca tatccagacc gatggttgcg ctctgcgcat tgctggcctg
    18181 aacgagaccg gaaacgatct caccgacctg gcgctcgaaa tactgcattt gattcgaacg
    18241 ttccgcctcg cggatacgct gctgaatgac ctggcgtgca gtctgtgcag cgacacggcc
    18301 aaaatctgcc ggggtggttt caactaaaac catatcaccc acctgggtat ctggctttac
    18361 cttttgagct tcacccaaca acacctcagt gcgcgcatcc acaaccgaat cttcgaccac
    18421 ttccttctca gcgtaaacag tcacttcgcc ggtgtcaggg tcaagtttgg cttctacatg
    18481 ctgcgcagtc gaagcgctga cagcgcgccg atacgcggaa accattgccg attcgattgc
    18541 ggcaatcacc acatccttgg caagttgttt ttcttccagc acttcattga atgccaatgc
    18601 aaattcgttt ttagccatgc tgcttactcc gttaactcct gttccttaca agcaaaaaag
    18661 tgggggtaac ccacttttcg atgtcttctc tattgggttt tccgtccgac gaaattttag
    18721 catagaggta tggaaggcgc aaggtgtcaa cataaatgaa tctactcctt gacatcatcg
    18781 catttctgcg taaaataccg cacaacatgt acacatccat tcgaaataaa aggccgcacc
    18841 atcacttttt cggaacagat gtgtttttat ttttgtgaac gagagagtcg cttg
//
LOCUS       DAOQJC010000076        18552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1388_length_18552_cov_7.821755, whole genome shotgun sequence.
ACCESSION   DAOQJC010000076 DAOQJC010000000
VERSION     DAOQJC010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18552
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1388_length_18552_cov_7.821755"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PLE39_12135"
     CDS             complement(<1..229)
                     /locus_tag="PLE39_12135"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNE69125.1"
                     /translation="MSVNHVVLGTGAIGRAAMMELVKRGESVRMVNRSGKMDEVPAGV
                     EVVAADLYDSAKVKEVTRGAKVVYQASQPEYT"
     gene            complement(242..916)
                     /locus_tag="PLE39_12140"
     CDS             complement(242..916)
                     /locus_tag="PLE39_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE69126.1"
                     /translation="MEIIKIKLSVSNAYLVKDRKSILVDTGSSHEADKILAEVKRAGV
                     NEKDISLILHTHGHVDHAGSTAELKRRLGVPVAVHAEDAFMLKTGTNGTVNPINFSAA
                     IYKQLLVKPFEPSQPDLEIDKEMTLKEFGVNARVVFTPGHTKGSLSILLGNKEAIIGD
                     VMMGGFMGGNLFPARPMVHYFFENFSEVRSSIKKVIEYKPSKLYVGHGGPLSLDAVIS
                     RFSNQI"
     gene            complement(918..1769)
                     /locus_tag="PLE39_12145"
     CDS             complement(918..1769)
                     /locus_tag="PLE39_12145"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE69127.1"
                     /translation="MNFAKSWMFNHPVTRRVLDGYFENDGIRQHYVTLGEGKLVVLIH
                     GFPEFWYSWRHQIEALSQDYQVVAIDQRGFNESGKPASTDGYRVERLVEDVAALINHL
                     GRKQAVIVGHDAGAWLAWHFAAMHPELTERLVILSVPHPNAMREELAEHGEQHRASEY
                     ARTMQKEGAKPMFRTWQISLLLDRLAFPLYLSADRGTDADAITSFYQFNYPQEPYTLD
                     ESLPMIEAPTLVIHGKRDRFLIASGHEHNARWLKHEPKTVMLDADHFVHQEKPDEVNR
                     IILDWLA"
     gene            complement(1766..2206)
                     /locus_tag="PLE39_12150"
     CDS             complement(1766..2206)
                     /locus_tag="PLE39_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69128.1"
                     /translation="MYTVSITRLRVRSIFLMPLFTLHAMRTSSQAQKAEGILGVETRF
                     EKNNVVWTKTIWTEESAMKKYRGSGAHQIAMRILSEMCSEASVTRWQQESTELPTWEE
                     AHRRLLAEGKLSKVKHPSPLQASGKTAPETMQSGFKMPAPPVTQ"
     gene            complement(2209..2901)
                     /locus_tag="PLE39_12155"
     CDS             complement(2209..2901)
                     /locus_tag="PLE39_12155"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE69129.1"
                     /translation="MVRPSKKTQIPNLQEAIKETAWKQIAETGAAALSLRAIARELNI
                     TAPAIYNYFPDRDALVTALIIDAYTSFGDYQIASRDSQAGDDLAGSLQAIGFAYRKWA
                     LEYPQRYQLIFGTPIPGYQAPIMEVLPSAARSLSALVSVIDKLRIANELNAEGFPSIQ
                     PGYEQMFNVWRGFAGDYDIFSLSVAMIIWSRVHGLVSLEISNNMPPFGVDGASLYRYE
                     MESIVRQFIKGN"
     gene            complement(3038..5002)
                     /locus_tag="PLE39_12160"
     CDS             complement(3038..5002)
                     /locus_tag="PLE39_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNE69130.1"
                     /translation="MINTNENKNSQTSIIELDIDYKPPRTLRSWLIGRPLSTADASHQ
                     TIGKVVGLAVFASDALSSTAYATQEILGVIAAAGTVAYGYLFPISLAIITLLAIVTIS
                     YEQTIHAYPGGGGAYIVARDNLGELPAQTAGAALLTDYILTVSVSISAGVAQIVSAYP
                     GLFPYRVAIAVIAVLLIMLVNLRGVKESGTAFAIPTYFFVVLMIATVGYGLFRYFTGS
                     LGMVIDAPHFEAHHVISVITPFILLHAFANGTTALTGVEAISNGITAFKEPRSKNAGI
                     TLIWMSLILGSLFLGLSFLTGKVHAVFSEEETVISQLARTIYDGRGIPYLLLISGTTV
                     ILIMAANTAFADFPRLGALHAGDGFLPRQLTYRGSRLVYSRGIVSLAVIASVLIIIFQ
                     ASVTRLIPLYAIGVFLSFTLSQAGMARRWWKIGHLKEGVEIVEPGSTLRYESGWQYRM
                     LINGFGALCTAVVMVVFAVTKFAEGAWVVLILTPILVAIFFAIHHHYKRLAKKLSLDN
                     FGAIPPHAIRHRVILPVSGVHQGTLAALRYARMLSDDVTAVHVTIEPAEAEKIRKKWE
                     TWGGGVRIVMLASPYRLFLEPLLGYISAISRQRQAGETITIVVPEFVSDGRVTALLHT
                     NTADLLRSQLKRQHGIVIINVPYHVHEWDG"
     gene            complement(5043..5684)
                     /locus_tag="PLE39_12165"
     CDS             complement(5043..5684)
                     /locus_tag="PLE39_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69131.1"
                     /translation="MKKVDLTPPVFALPPVSAVDLGTARRLIVLVPEIEMDLAYVARK
                     LGELTNSFNGCILFYGLCKDAVCELSLRRQLVALIAMMEAEKLSIESRIEIGENWPAS
                     VLTDLRQEDMVVCFPAPYPGFDRRPLHQFLSANLNCTVYMLDGVAHRETQSFAERASS
                     LMAWGGSIGLVFAFFWLQAKIVQLPQDWVHTSMLYFSLFAEVGAILGWNALFD"
     gene            complement(5813..6508)
                     /locus_tag="PLE39_12170"
     CDS             complement(5813..6508)
                     /locus_tag="PLE39_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE69132.1"
                     /translation="MSNSPHILVVDDELQIQRAIRTILTEKGFKVSTASSGNEGLTLA
                     AANVPDIVILDLGLPDMDGAEVCVRLREWTQCPIIILSVRDSERDKVSALDKGADDYL
                     TKPFGIEELLARVRVALRHAASQPGPQSKVVKAGQLTIDLAWHVVKLGEDEVKLTGTE
                     YKLLAYLAGNRGRVLTHQSILSHVWGPADADHTEYLRVYMRQLRKKLELDPERPRYIL
                     TEPGIGYRFIADE"
     gene            complement(6505..7959)
                     /locus_tag="PLE39_12175"
     CDS             complement(6505..7959)
                     /locus_tag="PLE39_12175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HNE69133.1"
                     /translation="MRTSPFLLRSSQYVLAFVVIAFVTAVLFVLREALDTTLIALLYL
                     IPLGLVTAFLGFGPGIASALMTFLAFNYFFIKPYYTFAVHRPTDVVILVIFLVVAIVI
                     SQLVGRMQVSLAAATAREREATQLYELSTALAGLHDEQVIVQILARQVQAVLLGEAVN
                     VHIVGGQSFQFSVLETGQPQRPPEWLVPIEAARGVLGEIRVWRAGPVLTSSEKRLLQT
                     FASQGAVALERARLVQAESRARVLEESDHLKSILLSSVSHELRTPLSTIKAAASSLRS
                     NEINWESPARPELIAAIDEEADHLNILVGNLLDMSRIESGALKPKREWNILAEIVEGV
                     LARMKHLLDEHILEVDVPESLPLVPVDYVQIEQVFTNLLSNGVKYAPAKTLIRVRAFV
                     EDDFVHVVVGNQGPQIPREHLERIFEKFFRITAADRVTGTGLGLSICKGIIEVHGGRL
                     WAENVPDGLAFNFTLPLIWEGVPPPKLPMDTETE"
     gene            complement(8017..9450)
                     /locus_tag="PLE39_12180"
     CDS             complement(8017..9450)
                     /locus_tag="PLE39_12180"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNE69134.1"
                     /translation="MRFQSQILSDTEKEQVHKEALQILQTTGAKFHSDKALKLLEDNG
                     ARVDREKRIAYIPPELVEQALASTPRSFVLGARNPKFDYPMPSPVTRFCLDGTGSFAQ
                     DFYTGEHRYGTLKDNENGLRVFQAMDMGVMAWPPTSAEDKPDYSRPLHEWAAMMKFCS
                     KHGEHELHFVNQTPYFAEMLVAVMGSEDEVRARHAYSLTYCPVSPLIHDGPMMDAYIE
                     MGSLDVPIMVLPMPAPGMTGPASLFGAICLADAEALSSLVTYQLAHPGRPMIFSSATG
                     TMDMRNGAFLGGTPEMGLMSAALIEMGRFYDLPSCSAGCTADAREPGADAVLEKVITT
                     IPPVLAGSDIVLGIGVVESDQLLVLEQIVVDNEIAHFCERIFQGVDSAPGRILTKDVQ
                     AVGPGGNFLSRKSTLEFARSKEVHFSDLLDRHTLDQWLQLGKPSMYSAARKKVEEILA
                     GPVLDPLSDETIGTLDEILIRADRELA"
     gene            complement(9455..10891)
                     /locus_tag="PLE39_12185"
     CDS             complement(9455..10891)
                     /locus_tag="PLE39_12185"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNE69135.1"
                     /translation="METDLKVLSSNEIAQIHERTLSILATTGVRVDTAKGRKFLKKAG
                     AEVNATTNVVRFPRGMVEECLQLAPRSFSLGGRRPNWEFPLNANLCSLVADGGASFAI
                     DEQTGERREANFQDWLGATRMIDSLDEVGVYWSMVDPGFFQKSAGDYVKYLHQMVGNF
                     SKHLQDCTHTIEESRWMLEVLGTVFGGKENVRKLRPFSYLVTPISPMVLEEQHTDAYL
                     EVIDWGVPVAIMPMPMMGGTAPASLISTILQANCETLAMLCLVQAASPGTPIIYAASP
                     AIMDPYTGRFTGSEVEHGLMGAAITEVARSYGLPVETSAGSTGHYAPGVRASYERALN
                     WVMATLSWPDLLVGPGQLGGSLLLSLEQLLIDVEVFHRSSRLSSGIDTRVKEWLEVDL
                     AAVGHGGNFLSRRSTREALRNGEVYMTDYDARNSYEAWAASGKPTILDELKPKVRKIL
                     DTHSPLPLDEDVERELERIEQRAREMRG"
     gene            complement(10922..12373)
                     /locus_tag="PLE39_12190"
     CDS             complement(10922..12373)
                     /locus_tag="PLE39_12190"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNE69136.1"
                     /translation="MSGNRPALEPLRSAFRIQYLTEQQLDTLQEATLDILENVGIKFP
                     SERALNVFIEHGADVDKNTQVVKIKRDMVFKAMKTVPRYFVMGARVPEYDLNLQEGTT
                     YFTTDGCGVEVVDLETRQYRPSSKADVGMMARVADYLPSIGFYWPMVSAQDCGRTSPL
                     HELEIGWNNTVKHFQSETIMGGREAAYAVEMATVITGSKEELRKRPPLSSLICTVSPL
                     MQDQPGIEAAMVFAEAGIPVGFLAMPTLGTTAPITQAGALAMADAEIISATVLMQLMF
                     PGAPIYHSMMQAWADMRTGGYVSYPRDARGRYGVVDMAHHWGMPSLGACYGTDSVVTG
                     TWQAAAEPALDPFLAGLASPEIVTGMGLSRTYTRLFPEQIILDDDLYQRARAYLQRME
                     VDEDSLAMEAIRNVGPGGHFLGQKHTRAHMKTSLVRGVTHELDAQNKYRDPVEVARER
                     VRWIIENHQPEPLEKSQQEELARIVAAADKELG"
     gene            complement(12427..13377)
                     /locus_tag="PLE39_12195"
     CDS             complement(12427..13377)
                     /locus_tag="PLE39_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE69137.1"
                     /translation="MNRPESNHPLVSELQASAATFQSASMGAGVWKRFRRHPGAIFGF
                     AIIFVLAVLTLLAPLSPYDPEASDLSERNNPPSLSHPMGTDALGRDLFTRVLFGGRIS
                     LTVGALVVVISLLIGVPIGALAGYYGGVLDGILMRFTDSFLSLPSFLVLILLSAILRE
                     VDAPLFEKNNVLTISMVIGVFGWTTFARLVRATFLTLRETDFISASRALGGSDLRIIA
                     RHILPNAIGPIIVEATLEFGYAIIEESGLSFLGFGIQPPTPSWGNLLSNAQEHFTKYP
                     WLAIFPGLMIFLSIISINYIGDGLRDAFDPYKVLEKVGEV"
     gene            complement(13381..14424)
                     /locus_tag="PLE39_12200"
     CDS             complement(13381..14424)
                     /locus_tag="PLE39_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE69138.1"
                     /translation="MATYIFRRLLQTAGLLALLSVLLFALVNLAPGGPLAGQGQSRHI
                     DPDKAELLKRQFGLDKPLPTQYLVWLVGNDWMQVDKDGDGVLDSYGTRQGILRGDFGF
                     SFRTRQPVLTEIGQRLPNTIYLMTVTMGVAVLIALPIGIISAIRQYSTFDITVTTFSF
                     AGQAIPEFWLGLILILIFYAWLKNPFTGEPLLPPGGMTSSNSTGFDLGDRVIHLILPV
                     ATGALGWVAWYSRFLRSSMLEVIHQDYIRTARAKGLSNLKVLSKHALRNALIPLVTLL
                     ALDLPYIFGGAVFVEILFAWPGMGRLYYQAALDRDYPILLAVLIIGAGFIILSNLLAD
                     IVYAYLDPRIRFE"
     gene            complement(14426..16123)
                     /locus_tag="PLE39_12205"
     CDS             complement(14426..16123)
                     /locus_tag="PLE39_12205"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE69139.1"
                     /translation="MYRVKLTSLFFIIILTATSLFGCQQNAPADSAQNSITIVIPEDP
                     PSFNPIIADTGYDALVMELVMLGLSDIDPEGKVFPELAAELPTLENGDVVVDDDAGTM
                     TVTWKMREDVQWADGTPVTADDVIFTYESIINPETGGWIPGIDYIDSVEKLDDHSFTI
                     SYNSIYPGYLTQFGGEQVVIWPAHYCDASQGFASWECARKPLSDGPFVLTEWVNGDHM
                     TFVKNEKYFEQGKPGIDAVTVRIIPDQSVRKTMLINGDADLDMWTNEQVVSDLQGEAN
                     VSVDPSPTNRWVMRIFFNLAAKGTTDPVATPHPILSDLRVRQAIRAAIDVDTISKEFF
                     LGYAKPAWTEFFRPPYTCDIPRPKFDPAAASALLEEAGWKDTDGDGVRECRGCSTAEE
                     GYKMEMEFITYSEYGEPLELTQQYIAEQLGNIGIKLNLTIVEGSVLWAASTDGGIEQS
                     GNFDMDIWDDGYSGVDPTDFLWSYYSTDAAVPDAGYNYSRWSNEQFDTLLGNVYTLDE
                     EQRQKDFCEMAKLLDAEIPQLLLFTASNADAHNARLQNVQSSTNDLVTWNSADWVLK"
     gene            complement(16327..>18552)
                     /locus_tag="PLE39_12210"
     CDS             complement(16327..>18552)
                     /locus_tag="PLE39_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE69140.1"
                     /translation="LEKETGVSTGFKPVGYVQAASSASRLRFLRKNAAFARSLGIEAQ
                     EISPSQFKDLWPLADPTGVTAGFYFPDDGRANPVDVTMSLAKGARDLGVRIFENVEVD
                     SVTKKMGRAVGVVTKTGESISAEYVINCTGMWGRKVGAQAGVSIPLQAAEHYYLLTEP
                     IEGVHANLPILEDVDTYSYFREEVGGLMLGLFEPVAKPWGVNGIPENFAFGEIQPDWD
                     RMMPYIEAAMERVPVLKTAGIRKLFCGPESFTADMTMLVGEAPELKNFWVAAGMNSLG
                     ILLGGGVGHTLAHWIVDGVPPLDVGEISLDRMLPYQNNQRYLNDRIVEILGMMYKETF
                     PNSQLQTARNVRKSILHEKLEKHGAFFASSAGWEYADWFAPEGREARVEKYSWDRQNW
                     FEYNAAEHKACREDVILMDTSMMSKFLVQGRDAQKMLDRISVSNINVPIGKIVYTEWL
                     NARGGIEADLTVTRIGEESFMVVCSDLAHRHVETWMQRHTETGEQVFIVDMTSAYALI
                     NVQGPKSRELLSRLSSADLSNEAFPYMSMKEIDVHYARALAFRVTYQGELGYELYIPT
                     EFAPTTFDALLEAGKDLGVKLAGMQALNTLRIEKGYRDYGHDIDGTDTPLEAGLSFLV
                     DFNKPNGFIGKEALLKQKEAGALKKRFVQLILDDPEPLLYYGEMIYRNGKIVGYVRSG
                     AYGFTLGGAVGLSIIEDDEPINEEYIKNGRFEVEVNNVMYSAKASLRPLYDPKNERVK
                     S"
BASE COUNT         4866 a         5078 c         4434 g         4174 t
ORIGIN      
        1 gagtgtattc tggctgcgat gcctgataca caacttttgc accgcgcgtc acttccttaa
       61 ccttggcaga gtcatacaga tctgcagcta ccacttccac cccggcaggg acttcgtcca
      121 ttttgcctga gcgattcacc atacgcacgg attcgccacg cttgaccaac tccatcattg
      181 cggcgcgacc gatcgcaccc gttcctaata cgacatgatt tactgacatg agatttatcc
      241 tttagatttg atttgaaaaa cgactaataa cggcatccag acttaaaggt ccgccatgcc
      301 cgacatataa cttcgatggt ttgtattcaa tgaccttctt gatactcgat ctaacttcgc
      361 taaaattctc aaagaaatag tgaaccattg ggcgggcagg gaaaagattg ccacccataa
      421 aacctcccat catcacatcg ccaataatgg cttccttatt tcctaagagg atcgagagcg
      481 aacctttggt gtgacccggc gtgaaaacta ctcttgcatt cacaccgaat tccttcaacg
      541 tcatttcttt atcaatttcg aggtctggct gactaggttc gaatggttta accaataatt
      601 gtttatagat ggctgcgcta aaattaattg gattgactgt tccgtttgta ccagtcttca
      661 acatgaacgc atcctctgca tggactgcaa ccggcacgcc cagcctgcgc ttgagttccg
      721 ccgtcgaacc ggcatgatct acatgcccat gggtatgcaa aataagggaa atatcttttt
      781 cgttcacacc tgcacgctta acttcagcaa gtatcttatc tgcttcatgg ctggaaccag
      841 tatcgacaag gatcgatttt ctatccttaa ccagataagc atttgacaca ctaagcttta
      901 tcttgatgat ttccatgtta tgcaagccaa tccaaaatga ttctattgac ctcatcaggc
      961 ttttcctgat gcacaaaatg atctgcatcc aacatcacgg tctttggttc gtgcttgagc
     1021 cagcgcgcat tatgttcgtg ccctgaagcg atcaagaaac ggtcacgttt cccgtgaatg
     1081 accaaggtcg gtgcttcgat catcggcagg ctttcatcca gcgtatacgg ctcttgcgga
     1141 taattgaatt gatagaacga agtaatcgcg tcggcatccg ttccacgatc agcagacaaa
     1201 tacaggggga acgcgagccg gtctaacagc aagctgatct gccaggtgcg aaacatcggc
     1261 tttgcacctt ccttttgcat tgtgcgggca tattcactgg cacggtgctg ctcaccatgc
     1321 tccgccaatt cttcacgcat cgcattcgga tggggaacgc tcaagatgac aagccgctcc
     1381 gtcaattcgg gatgcatcgc cgcgaaatgc catgccagcc acgcccccgc atcatgaccg
     1441 acgatcacag cctgcttccg tccgagatga tttatcaacg ccgcaacatc ttccaccaac
     1501 ctctccaccc gatacccatc tgtggatgca ggcttgcccg actcgttgaa tccgcgctgg
     1561 tcgattgcca caacttgata atcctgtgac aacgcttcga tctgatgccg ccatgaatac
     1621 caaaattcag ggaaaccgtg gatcaacaca accaacttgc cctcgcccaa ggtgacgtaa
     1681 tgctggcgga taccatcgtt ctcgaaatat ccatccagta cacgacgggt aacggggtga
     1741 ttgaacatcc aactttttgc gaaattcatt gcgtcaccgg cggagcaggc attttgaagc
     1801 ctgactgcat agtttcagga gcggtcttcc cagaagcctg cagcggtgaa gggtgcttca
     1861 cctttgaaag cttcccttcc gccaacaaac ggcgatgtgc ttcttcccac gtgggaagtt
     1921 cagtgctttc ctgctgccaa cgtgtaaccg aagcttcaga gcacatctca gaaaggatac
     1981 gcatggcgat ctggtgtgcg cccgagccgc gatacttttt catcgcggat tcttccgtcc
     2041 agatggtctt ggtccaaacg acgttatttt tttcaaagcg ggtttccacg ccaaggatgc
     2101 cttctgcttt ttgtgcctgc gaactggtgc gcatggcatg aagtgtaaac aacggcatga
     2161 gaaagataga acggactcgc aagcgggtaa tggatacagt gtacatttct aatttccttt
     2221 tataaattga cgaacgatgg attccatttc ataacgatag agtgaagcgc catccacacc
     2281 aaagggtggc atattgttgg agatctccaa tgagacgaga ccatgaacgc ggctccaaat
     2341 aatcatggca accgatagcg aaaagatgtc gtaatcccct gcaaaaccgc gccacacgtt
     2401 gaacatttgc tcataaccag gttgaatgga aggaaaaccc tctgcattca actcattggc
     2461 tatccgcagt ttgtcaatca cactgacaag agcgcttaaa gaacgtgctg cggaaggcaa
     2521 gacttccatt atcggcgctt ggtagccagg aatgggcgta ccaaaaatca gctggtaacg
     2581 ttgcggatat tccagcgccc actttcggta ggcaaagcct atagcttgca aactccccgc
     2641 taagtcgtcc cctgcctggg agtcgcgtga ggcgatttga taatctccaa aagaagtgta
     2701 ggcatcaata atgagcgcgg tgacgagcgc gtcgcggtct ggaaagtagt tgtagatggc
     2761 aggcgcagtg atgttgagtt cgcgagcgat cgcgcgcaag gacaaagctg ctgcacctgt
     2821 ctctgcgatc tgcttccatg ctgtttcttt gatggcttcc tgcaaattgg gtatttgagt
     2881 tttcttgctg ggtctgacca tgtcggctcc ttatttacta tacagtgtaa atatacagtg
     2941 tatagtaaat attgtcaagg cctaattggg acagaaaaac acagccgact ttctgtatga
     3001 aagtcggctg cgagtactta cagctttttg gtccaactta cccgtcccat tcatgcacat
     3061 gatacggcac attgatgatc acaatgccat gctgccgttt caactggctg cgaagtagat
     3121 ccgcggtatt tgtgtgcaac agagctgtga cacgcccatc agaaacgaac tccgggacaa
     3181 cgatggtgat ggtctcaccc gcctgacgct ggcgggatat cgccgatata tagcccaaca
     3241 gcggttccaa aaagaggcga taaggagatg ccaacatcac aatgcggacc cctcctcccc
     3301 atgtctccca cttcttgcga attttttcag cctctgcagg ttcaattgtg acatgcacag
     3361 ccgtaacatc atcagacaac atccgcgcat aacgcagcgc agccagtgtg ccctgatgta
     3421 ccccactcac cggcaaaatg acacgatgtc gtatcgcatg cggaggaatg gcgccaaaat
     3481 tatccagcga gagttttttg gcaagccttt tatagtgatg atgaatggca aagaagatcg
     3541 ccacaaggat cggtgtaaga ataagtacaa cccaggctcc ctctgcgaat ttcgtcactg
     3601 caaacacaac catcaccacc gccgtacaga gagcaccaaa gccgttgatc aacatcctgt
     3661 attgccaccc gctttcatat ctcagtgttg aacctggctc aacaatttcc acgccttctt
     3721 tgaggtgacc gatcttccac caacgacgcg ccatgcctgc ctgtgaaagg gtgaaggaaa
     3781 ggaaaacccc gatcgcataa agcggaatca agcgagtcac acttgcctga aaaatgatga
     3841 tcaaaacgga agcgatcact gccagtgaca caatgccgcg agaatagacc agccgactcc
     3901 cgcgatacgt aagctggcgt ggaaggaacc catcacctgc atgaagtgct ccaaggcggg
     3961 gaaaatctgc aaatgcagta ttcgcagcca tgatcaaaat aacagttgta ccggagatca
     4021 ataacagata cggaatcccc ctgccgtcat agatggtccg cgccaattgg gaaatgacgg
     4081 tttcctcttc cgaaaacaca gcatgcactt ttccagtaag gaacgaaagc ccgaggaaca
     4141 aagaacccag aatcagcgac atccagatca gggtaatgcc tgcattcttg ctgcgaggtt
     4201 ccttgaaggc ggttatgcca ttggagatgg cttccacacc ggtcaaggca gtagtgccat
     4261 tggcaaatgc atggagaaga atgaaaggtg taatcaccga tatgacatgg tgagcttcga
     4321 aatgcggagc atcaatcacc atacccaacg aaccggtaaa gtaacggaac aacccatagc
     4381 ccaccgtggc gatcattaaa accacaaaaa aataagttgg aatggcaaaa gccgtaccag
     4441 actctttcac acccctcaaa ttcaccaaca tgataagcag tacggcaata accgcgattg
     4501 ccacccgata cggaaataaa cccgggtagg cagagacaat ctgtgcaacc cctgccgaaa
     4561 tagacacaga cacagtcaaa atataatcag tgagaagtgc tgcgccagcg gtttgggcgg
     4621 gcaattcgcc aagattatca cgggctacaa tgtatgcgcc accgccaccg gggtaggcat
     4681 gaatcgtctg ctcgtaagaa atggtcacaa ttgccaagag ggtgataatg gcaagggaaa
     4741 tgggaaaaag atatccgtat gccactgtgc cagccgctgc aattacaccc aatatttcct
     4801 gagtggcata cgcagtggag gaaagtgcat cagaagcaaa cacagccagc ccaaccacct
     4861 tcccaatggt ttgatgagat gcatccgcag tggataaggg acggccaata agccatgaac
     4921 gcagggtgcg tggaggtttg tagtctatat ccaattcaat aatggaagtt tgtgagtttt
     4981 tattttcgtt tgtattaatc atatcgtcat cacttcagtt aattcatgtt tttacgaccg
     5041 cactaatcaa acaatgcatt ccagcccaaa atggccccga cttcggcgaa aagggaaaaa
     5101 tacagcatgg acgtatgaac ccaatcttgc ggcagttgaa caatttttgc ctgcagccaa
     5161 aagaaagcaa acacaagacc aatggagccg ccccaagcca tcaggctgga agccctctca
     5221 gcaaatgact gggtctcacg atgcgcaaca ccatcaagca tgtaaacagt gcaattcaaa
     5281 tttgcggaaa ggaattgatg aaggggcctg cgatcaaagc ctgggtatgg cgcggggaaa
     5341 cagacaacca tatcttcctg ccgaagatcg gtcaaaacgg aggcgggcca gttctctccg
     5401 atctcaattc tcgattcgat ggaaagtttt tccgcttcca tcatcgcaat taaagcgaca
     5461 agctgcctgc gcaggctcag ctcgcaaaca gcatccttac aaagaccgta aaacaaaata
     5521 cagccgttga aggaatttgt taattctcca agtttccgtg caacataggc aagatccatc
     5581 tcaatttcag gtaccagcac gattaatcga cgggcagtcc caaggtcaac ggcggaaacg
     5641 gggggaaggg caaaaacggg aggagtcaaa tccacttttt tcatttcacc tcacagataa
     5701 gtgaatccta tccctctgat ataaagcgat gacaaaaatt ccaggcaatt gtataaagaa
     5761 caaataaaaa cgcccttttg caagggcgtt gtaaggcgtg gggtggtcca gcttattcat
     5821 ccgcaataaa gcgatagcca ataccaggtt ctgtgagaat gtaacgcgga cgttcgggat
     5881 cgagttccag tttcttgcga agctgccgca tataaacacg caggtattca gtatgatctg
     5941 catcagcggg cccccaaaca tgtgaaagga tgctctgatg cgtaagcacg cgtccgcggt
     6001 tacccgccaa atacgccagc aatttatatt cagtgcctgt caatttcact tcatcttcac
     6061 caagcttgac aacatgccag gccagatcaa tcgtcaactg cccggctttg acaaccttac
     6121 tctgaggccc cggctggctc gccgcatgcc gcaaggccac acgtacacgc gccagcagtt
     6181 cttctatgcc aaagggtttc gtcaggtagt catctgcacc tttatcgagc gcagaaactt
     6241 tatcacgttc gctatcgcgt acagaaagaa tgatgatcgg gcattgagtc cactcgcgca
     6301 agcggacaca aacctcagca ccatccatat ccggcaggcc cagatcaagg atcacaatat
     6361 ccggcacatt tgccgcagcc aaagtgagac cctcgtttcc gctgcttgca gtggaaacct
     6421 tgaagccctt ttcggtcaag atcgtgcgga tggcacgctg gatctgcaat tcgtcatcca
     6481 ctacgagaat gtgcggtgaa ttactcattc ggtctccgta tccatcggaa gtttgggcgg
     6541 cggcacgcct tcccaaatca ggggcagggt aaagttgaag gccaatccat caggaacatt
     6601 ctcagcccaa aggcgcccgc catgtacctc gataatccct ttgcaaattg aaaggccaag
     6661 gccggtacct gtaacccggt cggcggcagt gattcgaaag aatttttcaa agatcctttc
     6721 aaggtgctcc cttggaattt gcggaccctg attgccaacc accacgtgaa caaaatcatc
     6781 ttccacgaaa gcacgcaccc gtatcaaagt ttttgcaggt gcgtatttca ctccattact
     6841 caacagattc gtaaatacct gctcgatttg gacatagtcc acaggtacaa gcggaaggct
     6901 ctcgggcacg tccacttcaa ggatatgttc atccaaaaga tgtttcatgc gcgccagcac
     6961 accttcaacg atctcagcca gaatattcca ttcccgtttg ggtttaagcg cacccgactc
     7021 gatccgcgac atatccaaca ggtttccgac cagaatattt aaatggtcgg cttcttcgtc
     7081 aatggcggcg atcaactccg gtcgtgcagg cgattcccaa ttgatttcat tgctgcgcag
     7141 actcgaggct gccgctttaa tcgtggaaag aggggttcgc aattcatgcg aaacagaaga
     7201 caaaagaatg gatttcaaat ggtcactctc ttcaaggact cgcgcacgtg actcggcctg
     7261 aacaagccgc gcccgttcaa gtgccacggc cccctgactg gcaaaagtct gcaataaacg
     7321 cttctcactg gacgtaagaa ctggtcctgc tctccaaaca cgtatctccc cgagaacacc
     7381 tcgtgctgcc tcaatgggca ccaaccattc gggagggcgc tgcggttgac cggtttcgag
     7441 cacgctaaac tgaaaagatt gtccacccac aatatggacg ttaaccgcct cacccagcaa
     7501 tactgcctgt acctgtctgg ctaatatttg aacaatgacc tgttcatcat gcaagcccgc
     7561 cagagcggtg ctcaattcgt aaagctgggt ggcttcgcgt tcgcgggcag tcgctgcagc
     7621 cagacttacc tgcatacgcc ccaccaactg gctgatcaca attgctacaa caagaaagat
     7681 gacaagaatt acgacatctg ttgggcgatg gacagcaaag gtgtaataag gtttgataaa
     7741 aaagtagtta aatgcaagaa aggtcatcaa tgcgctggcg atccctgggc caaaccccaa
     7801 aaaagcagtg accaacccca gcgggattaa gtacaacaat gcaatcaggg ttgtgtccaa
     7861 cgcctcgcgc aagacaaaca agaccgccgt cacaaaggcg atcacaacaa aagccagaac
     7921 atattgagac gaacgaagaa ggaaaggtga agttcgcatt ggggcaaagg agattatacc
     7981 ttttgcctgc cgggcaaaac ttaccaacga tcaagatcag gcaagttcgc ggtctgccct
     8041 gatcagaatc tcatccaacg tgcctatcgt ttcatccgaa agcggatcaa gcaccggccc
     8101 ggcaagaatc tcttccactt ttttacgtgc agcagaatac atggatggct tgcctagctg
     8161 caaccattgg tcgagggtat gacggtccaa caggtcggaa aagtgaacct ccttgctgcg
     8221 ggcaaattca agcgtggatt tgcgggagag aaaatttcca cccgggccaa cggcctgaac
     8281 atcttttgtc agtattcttc cgggagcgga atccactcct tgaaaaatgc gttcgcaaaa
     8341 atgcgcgatt tcattatcca ccacgatctg ctcgagaacg agaagctggt cactttcaac
     8401 aacaccaatg ccgaggacga tatctgaccc tgccaacact ggcgggatgg tggtgatgac
     8461 cttttcgagc acagcatctg cccctggctc gcgtgcgtcg gctgtgcaac ccgctgaaca
     8521 ggaaggaaga tcgtagaaac gtcccatttc gatcagcgcg gcagacatca atcccatttc
     8581 gggagtacca cccaaaaacg caccgttccg catatccatg gtgccggtgg cgctgctgaa
     8641 tatcatcggg cggccagggt gcgccagttg ataggtgacg agtgaagaaa gggcttcagc
     8701 atcggccaga cagatcgcgc caaataagct ggcagggcct gtcataccag gtgcgggcat
     8761 gggcaacacc atgatgggaa catccaaact tcccatttcg atataggcat ccatcatggg
     8821 gccgtcgtgt atgagcggtg aaacagggca ataggtcaat gagtaggcat ggcgggcgcg
     8881 gacttcatct tcactgccca tgacggcaac aagcatttct gcaaaatacg gggtctgatt
     8941 gacgaagtgc aattcgtgtt cgccatgttt tgaacaaaac ttcatcatcg cagcccattc
     9001 atgtaacgga cgggaataat cgggcttgtc ttcggcagag gttgggggcc aggccatgac
     9061 gcccatatcc atggcttgaa aaacgcgcaa tccattctca ttatctttga gggtaccgta
     9121 gcggtgttcg cctgtgtaaa aatcctgtgc gaaggagccc gtgccatcca gacaaaagcg
     9181 ggtcacaggc gatggcatgg ggtaatcaaa tttcgggtta cgtgccccaa gcacaaaaga
     9241 cctgggagtg gatgcaagtg cctgttccac caattcagga ggaatgtagg caatgcgctt
     9301 ttcacgatcc acacgtgcgc cattgtcttc gagaagtttc aaggctttgt cgctatgaaa
     9361 ctttgcgccc gtagtttgca gaatttgtaa tgcctccttg tgaacctgct ctttttcagt
     9421 atcacttaga atctgacttt gaaaacgcat aaagttatcc tctcatttca cgggcacgct
     9481 gttcaattct ttcaagctcg cgttcaacat cttcatcaag cggcaggggt gaatgtgtgt
     9541 cgagaatctt ccgcaccttg ggtttcaact catccagaat ggtcggttta ccggaggcag
     9601 cccaggcttc gtaggaattt cgggcgtcgt aatcggtcat atacacttcc ccatttcgca
     9661 atgcctcgcg ggtggaacgt cgactcagga agttaccgcc atgcccaacg gcagccagat
     9721 ccacttcaag ccattccttg acccgcgtat caattcctga tgaaagccgt gaagaacgat
     9781 gaaacacctc cacgtcaatt aataactgtt ccaggctcaa caacaacgac ccgcccagtt
     9841 gaccaggccc caccaacaga tcaggccagg acagtgtagc catgacccaa ttgagagcgc
     9901 gttcataact ggcacgtaca ccgggcgcgt aatgccctgt gcttccagcc gaggtctcca
     9961 ccggcaggcc gtaggaacgt gctacttcag tgatggcggc tcccatcagt ccatgttcca
    10021 cttcactgcc tgtgaaacgg cccgtgtatg ggtccatgat ggcaggcgag gcggcataga
    10081 tgatgggcgt ccccggtgat gcggcctgca ccaaacacag catggcgagc gtctcgcagt
    10141 tcgcctgcaa aatcgtggaa atcaaactgg cgggtgccgt tccacccatc attggcatgg
    10201 gcatgatcgc cacaggcacg ccccaatcga tcacttcaag gtaggcatcg gtgtgttgtt
    10261 cttccagcac catgggcgag atgggcgtga ccagataaga gaagggacgc aatttacgta
    10321 cattctcttt tccgccaaat acagttccca gcacttccaa catccagcgt gattcttcga
    10381 tggtgtgggt gcaatcctgc aggtgttttg agaaattccc caccatttga tgaaggtatt
    10441 tcacataatc gccagcggat ttttgaaaaa agcccgggtc caccattgac cagtacaccc
    10501 ccacttcatc caacgaatcg atcatgcggg tggcacccag ccaatcttga aaattcgcct
    10561 cccgccgctc gccggtctgc tcgtcaattg caaaagatgc accgccatcg gcaaccagcg
    10621 aacaaaggtt tgcattcaac ggaaattccc aattcgggcg cctgccgccc aacgaaaaac
    10681 tgcgcggcgc caattgcaga cattcttcga ccatcccgcg tggaaaacga accacattcg
    10741 tagtggcgtt tacttccgcg cccgcttttt tgaggaattt cctacccttg gccgtatcca
    10801 cccgcacgcc ggtggttgcc agaatggaaa gcgtacgctc gtgaatctgc gcgatctcat
    10861 tactcgacaa aaccttcaag tctgtttcca taatctgctt tccaactgca acctgccaaa
    10921 gctaccctaa ctctttatct gcggcagcga caatacgagc caattcttcc tgctggcttt
    10981 tttcgagcgg ttcgggttgg tgattttcga tgatccagcg cacacgttca cgcgccactt
    11041 caaccgggtc acggtatttg ttctgcgcat ccaattcatg ggtcacgccg cgcacaagcg
    11101 aggtcttcat atgggcgcgg gtatgctttt gcccgaggaa atgcccgccc ggccccacat
    11161 ttcggatcgc ttccatcgca agcgagtctt catcaacttc catgcgttgg agataggcac
    11221 gggcacgttg gtacaaatca tcatccagaa tgatctgttc ggggaataaa cgcgtataag
    11281 tgcgcgagag ccccatgcca gtgacgatct ccgggctggc aagcccagcc aaaaatggat
    11341 cgagtgcagg ctctgcggca gcctgccatg tgccagtaac aactgagtcg gtgccgtaac
    11401 atgcgcccag tgaaggcatc ccccaatgat gcgccatatc caccacgcca tagcgtccac
    11461 gtgcatcacg cggatagctg acataaccac ccgtgcgcat atcagcccag gcctgcatca
    11521 tcgaatgata aataggcgcg ccggggaaca tcaactgcat gaggactgtg gcgctgatga
    11581 tctctgcatc tgccatggcc aaggcccccg cctgtgtgat cggagcggtc gtccctaacg
    11641 tgggcatggc caaaaagccg acaggaatcc ccgcctcggc aaaaaccatc gcggcctcaa
    11701 tgccgggctg gtcctgcatc agcggagaga ccgtacagat caaagacgaa agcggcggac
    11761 gtttgcgtaa ctcctctttg ctgccagtga tgacagtcgc catttccacg gcataagcgg
    11821 cttcacgccc acccataatg gtctctgatt gaaagtgctt gaccgtgtta ttccacccga
    11881 tctccagttc atgcaaaggg gaagtgcggc cgcaatcctg tgcgctgacc atgggccaat
    11941 aaaacccgat cgaggggaga taatctgcaa cccgcgccat catacccaca tcagccttgc
    12001 ttgaagggcg gtattggcgt gtttcaagat ccacaacctc caccccgcag ccatcggtgg
    12061 tgaaataggt cgtcccttcc tgaaggttga ggtcatattc gggcacacgc gcacccatga
    12121 cgaaataacg cggcacggtt ttcatcgctt tgaaaaccat gtcgcgttta attttgacca
    12181 cctgggtatt tttatccaca tcggcgccgt gttcgatgaa cacattcaag gcgcgctcgg
    12241 aagggaattt tatgccgaca ttttcaagaa tatcaagtgt ggcttcttga agtgtatcca
    12301 gttgctgttc tgtaagatat tgaattcgaa atgcggaacg aagcggttca agtgcaggac
    12361 gattgccaga cataaaatac tctcctttat tcgaatggga tgattcaaac ggctaaagcc
    12421 gtaaaactac acttcgccaa ctttttcaag caccttgtat gggtcaaagg catcgcgcaa
    12481 gccatcgccg atgtaattta tggaaataat actgaggaag atcatcaacc cggggaagat
    12541 cgccagccag ggatatttcg taaaatgctc ctgcgcattg ctcaacaaat taccccatga
    12601 aggcgtgggc ggctgaatac cgaatcccag aaaacttaat cccgactctt cgatgatggc
    12661 gtatccgaat tcaagggttg cctctacaat gatcggaccg atggcattcg gcagaatgtg
    12721 cctcgcaatg attcgaaggt cagatccacc cagggcacgc gaagcagata taaagtccgt
    12781 ttctcgaagc gtgaggaagg tcgcccgcac cagtcgcgca aacgtcgtcc atccaaaaac
    12841 accaatgacc atgctgatcg tcaggacgtt gttcttttca aagagcggag catccacttc
    12901 acgcagaatt gcactcagca aaatgagcac aagaaaagaa ggcagtgaaa ggaaagaatc
    12961 ggtaaaacgc atcagaatgc catccaaaac gccgccataa tagccagcca gcgcaccaat
    13021 gggcacaccg atcaaaagcg agatgacaac aaccaacgcg cctaccgtca gcgaaatgcg
    13081 cccgccgaac aacacacggg tgaacaaatc acgcccaagt gcatcggtgc ccattgggtg
    13141 gctcagggat ggcgggttgt ttcgttctga aagatccgaa gcctcggggt catagggtga
    13201 aagcggcgcc aacagcgtta acactgccaa aacgaatatg atggcaaaac cgaagatcgc
    13261 acccgggtgg cggcggaagc gtttccatac tcctgcgccc atgctggccg attgaaacgt
    13321 ggcggcggat gcctgtagct cactcacaag tggatgattg gattctggtc tgttcatgag
    13381 ctactcaaat cggatgcggg gatcaagata ggcatagacg atatcagcca gcaggttcga
    13441 aagaatgatg aaccctgcgc caatgatcag caccgccaac aaaatggggt aatcgcggtc
    13501 gagcgccgcc tgataataca acctgcccat accaggccac gcaaaaagga tctccacaaa
    13561 gaccgcgccg ccgaaaatat aaggcaggtc caacgccagc aaagtgacaa gcggaatcag
    13621 cgcattgcgc aaagcatgtt tggacaagac cttgagattc gaaagccctt tggcacgcgc
    13681 agtgcggatg taatcctgat ggatcacctc cagcatgctt gaacgaagga aacgcgaata
    13741 ccatgccacc caacccagcg caccagtcgc cacaggcaga atcaggtgaa tgacacggtc
    13801 gccaaggtcg aaaccagtgc tgttggatga ggtcatgccg cccggaggca gcaatggttc
    13861 acccgtgaat ggatttttga gccaggcata aaaaatcaga atcaggatca agcccagcca
    13921 aaattcagga atcgcctgcc ctgcaaacga aaaggttgtc accgtaatat caaaggtgga
    13981 atactgacga atggcagaaa tgattccaat gggcagcgcg atcaacaccg ccacacccat
    14041 cgtgaccgtc atcaagtaga tggtattcgg cagccgctga ccgatttccg tcaatacagg
    14101 ctgacgggtg cggaaggaaa agccaaaatc gccgcgcaaa attccctgcc gcgtgccgta
    14161 actatctaaa acgccatcgc catctttgtc cacctgcatc cagtcgttgc cgacaagcca
    14221 gacaagatat tgcgtcggca ggggtttatc cagcccaaat tgacgcttca gcaattcagc
    14281 cttgtcagga tcaatgtgcc ggctctgccc ctgacccgca agcggtcccc ccggcgcaag
    14341 attgaccagc gcaaaaagca ggacactcag caaagccagc aggccagccg tttgcaataa
    14401 acgcctgaaa atgtaggtag ccaagttatt tcaacaccca atcggcggaa ttccacgtca
    14461 caagatcgtt cgtggaggat tgtacatttt gcagacgggc attgtgcgca tctgcatttg
    14521 aagctgtgaa cagcaacaac tgcggaattt cagcatccag cagtttggcc atttcacaga
    14581 aatccttctg acgttgttcc tcatccagtg tgtacacatt gcccaacagg gtatcaaact
    14641 gttcatttga ccaacggctg tagttatagc cagcatcggg cacggctgca tccgtggaat
    14701 aataactcca gagaaaatcg gttgggtcca cgcctgaata accgtcatcc caaatatcca
    14761 tgtcaaaatt accgctttgt tcaataccgc catcggtgga agccgcccac aagacgctgc
    14821 cttccacaat ggtcaggttg agtttgattc cgatattgcc cagctgctcg gcaatatact
    14881 gctgggtcaa ttccaaaggc tcgccatatt cagagtaggt gatgaattcc atctccatct
    14941 tgtagccttc ttcagcggtg gaacagccgc ggcattcacg caccccatcg ccatcagtat
    15001 ccttccagcc tgcttcctcc agcagagcag atgcggcagc ggggtcaaat ttcgggcgcg
    15061 gaatatcgca agtgtacggc gggcggaaga attccgtcca ggcgggcttg gcatacccga
    15121 ggaaaaactc cttcgaaatc gtatccacat cgatcgcagc acgaatcgcc tgccgaacgc
    15181 gcagatcaga caaaatcggg tgcggagtcg ctacagggtc ggtggtgccc ttcgccgcca
    15241 gattgaaaaa gatacgcatc acccagcgat tcgtggggct ggggtccacg ctcacattgg
    15301 cctcaccctg caggtctgaa accacctgtt cattcgtcca catatccaga tcggcatcgc
    15361 cgttaatgag catggtcttg cgcaccgatt gatcggggat gatccgcact gtaaccgcgt
    15421 caatccctgg cttgccctgc tcaaaatatt tctcgttttt cacaaacgtc atgtggtcgc
    15481 cgttcaccca ctcggtcaac acgaatgggc catcgctcaa tggcttgcgg gcacactccc
    15541 atgaagcaaa cccctgtgag gcatcgcaat aatgcgcagg ccagatcaca acctgttccc
    15601 cgccaaactg tgtcaagtag cctgggtaaa tgctgttgta ggaaatcgtg aacgagtgat
    15661 cgtccaactt ctccacactg tcaatgtaat caatgcctgg gatccagccg ccagtttcgg
    15721 gattaatgat cgattcgtag gtaaagatca catcatcagc cgtaacaggc gtcccatccg
    15781 cccactgcac atcctcgcgc atcttccatg tcacggtcat tgtccccgca tcgtcatcaa
    15841 caaccacatc gccattttca agggtcggca attccgccgc cagctcgggg aagaccttgc
    15901 cctcagggtc aatgtcactc aaacctaaca tcaccaattc catgacaagg gcatcatacc
    15961 cagtatcagc gatgatgggg ttgaaagaag gcgggtcttc aggaatgacg atcgtaatgc
    16021 tattctgcgc cgaatcggca ggagcattct gctggcaccc aaacaggctg gtcgccgtca
    16081 atataataat aaaaaacaac gatgtaagtt ttactctgta catctctcct ccgatttacc
    16141 ttggatttgt tattacgatt atatgtgccg tactttactt cccaaaatta ttgcatactt
    16201 tacccaatat gtcatgtgat tttcatctct gtttcgcgga tatgaacccg catcccgccc
    16261 agccgatgat tgcctcagcg tcaaaaaaat tctctccccg aaaacgcgga cttaagtccg
    16321 cattcctcag gacttcactc tttcattctt cgggtcatac agcgggcgca atgacgcttt
    16381 tgcagaatac atcacattat tcacctcaac ctcaaacctg ccattcttga tgtactcctc
    16441 attgatcggc tcatcatctt cgataatgga caaacccacc gcaccaccca acgtgaagcc
    16501 ataagccccc gagcggacat accccacgat cttcccgttg cgatagatca tttcaccata
    16561 atacaacaat ggttcggggt catccaaaat caattgcaca aaacgctttt tgagcgcacc
    16621 cgcttctttt tgtttcaaca gcgcctcttt accgatgaat ccattcggct tgttgaaatc
    16681 cacaaggaaa ctcaaacccg cttcaagcgg tgtatcggtg ccgtcgatat catggccgta
    16741 atcgcgatag cctttttcaa tgcgaagggt attgagtgcc tgcatccccg ccagttttac
    16801 acccaaatcc ttccccgctt cgagaagcgc atcgaaggtc gtcggagcaa actcggtggg
    16861 gatgtacaac tcgtacccca actcgccctg atacgtcaca cggaaggcca atgcgcgtgc
    16921 gtaatgcacg tcaatttctt tcatggacat gtagggaaag gcctcgttcg atagatctgc
    16981 actggaaaga cggctgagta attcgcgtga ttttggtccc tgcacgttga tcaaggcgta
    17041 cgctgaagtc atatccacaa tgaagacctg ttcccctgtt tcagtgtgcc gctgcatcca
    17101 tgtttccaca tggcgatgtg caaggtcaga gcaaacgacc atgaaggatt cttcgccaat
    17161 gcgggtaacg gtcaggtccg cttcgatgcc gccacgggca ttcaaccact cggtatagac
    17221 gatctttccg atcggcacat tgatattgct gacggagatc cgatcgagca ttttttgcgc
    17281 atcgcgcccc tgcaccagaa atttggacat catcgaagtg tccatgagaa tcacatcttc
    17341 gcggcaggct ttgtgttcgg cggcgttgta ttcgaaccag ttttgcctgt cccaactgta
    17401 tttttcaacc ctggcttccc tgccttcagg agcaaaccaa tctgcatatt cccagcctgc
    17461 agaagaagca aaaaaagcgc catgtttttc cagcttttca tgcaaaatgg attttcgcac
    17521 gttgcgcgca gtttgcagct gcgaatttgg gaaagtctcc ttgtacatca tgccaaggat
    17581 ttccacgatg cggtcgttca aatatcgttg gttgttttga tatggcagca ttcggtcaag
    17641 gctgatctcg cccacatcca atggcggaac tccatccacg atccaatgcg caagggtgtg
    17701 accgaccccg ccgccaagca aaataccgag cgaattcatg cctgccgcca cccagaaatt
    17761 tttcaactcg ggggcttccc caaccagcat ggtcatatcg gcggtgaaac tttcggggcc
    17821 gcaaaacagt ttacgaatgc ccgctgtttt caacacgggc acacgctcca tggcggcttc
    17881 gatgtaaggc atcatacgat cccaatcggg ttgaatttcg ccaaaggcaa agttttcagg
    17941 aataccgttc acgccccagg gtttggcaac gggctcaaac aaccccagca tcaatccacc
    18001 cacttcttca cggaagtagg agtatgtgtc tacatcttcg aggatgggca ggttggcatg
    18061 gacgccttca atgggttcgg tcagcaaata ataatgttcg gcagcctgca atgggatgct
    18121 cacccccgcc tgtgcgccga ctttacgtcc ccacatgcct gtgcagttaa tgacatattc
    18181 ggctgaaatg gattcgcccg tcttcgtcac cacaccaacg gcgcggccca ttttcttcgt
    18241 aacagaatca acttcaacgt tttcaaagat gcgcacgccc aaatcgcggg cgcctttggc
    18301 caggctcatg gtcacatcca cggggttggc gcgcccgtca tcggggaaat aaaatccagc
    18361 cgttacacct gtggggtctg ccaatggcca aagatctttg aactgtgacg gggaaatttc
    18421 ctgcgcctcg attccaagcg aacgggcaaa cgcggcgttc tttcgaagaa agcgcaaacg
    18481 cgaagcggaa cttgccgcct gcacataccc aacaggtttg aagcctgtgg aaacgccagt
    18541 ctctttttca ag
//
LOCUS       DAOQJC010000077        18457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1403_length_18457_cov_9.862243, whole genome shotgun sequence.
ACCESSION   DAOQJC010000077 DAOQJC010000000
VERSION     DAOQJC010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18457
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1403_length_18457_cov_9.862243"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2626)
                     /locus_tag="PLE39_12215"
     CDS             complement(<1..2626)
                     /locus_tag="PLE39_12215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE69141.1"
                     /translation="MLDLFFSVAMDALIAFDESGRVIRANQFAAEMFGYSMDEFQTMT
                     VENLLPKKNAKNRKDLVLLLLNTNVENRKIVKSHRVWVLHKSGYEFLVMASIGKGSLN
                     DQPVSLISLRDSLIDGQEENLTDLPITDARENPNAEIRIGRDGRILFANKSGKRLLRR
                     SGMVVTGQVPSEWVGFFEQALKRKTHIQRVIQHGRRLYSCHFNPTPEMGYVTLYALDI
                     TEYEAEKNRLALSDEILRSIGNLVLVANSNAEVVYISPSVKEILGYEPEELIGNGWWE
                     LERISGGDVLAERDYISKAAAGLRQVDDKPYEHRVRHKNGDWRWLMLADRKGPRDLLI
                     GIGTDVTESKKTGGELQNQRDFAQTLTTQMGQGLTVIDEEGHFTFANPYSAKILGYET
                     EQLAGLTAFDVTFPEDHELVKTVLSKRNVGEVTTLEIRLRGRNGDEVYALVTSVPHVV
                     DGQFAGTINVITDLTERRQMENLLRDYATAIEKSNIDLADARDHALEASYLKSSFLAT
                     MSHEIRTPMNAIMGMTEMLLSTDLNDEQREFAQTIDSSTQGLLAILNDILDFSKIEAG
                     KLSIIPSVFNPVEMTRDLIKLFQPRAQEKNITLSMMATASIPETLVGDGGRIRQILGN
                     FVSNAIKFTEKNGCVFINISGTSINQNTLMTTFTVQDNGIGVSDMMRSKLFEPFTQAD
                     GSNTRKHGGTGLGLAISRRLVDLMHGEIGFESFEGTGSTFWFSLPLYTTAPKDQEETE
                     FSSRGMQKKYLNYSSRKPILIVEDNLVNRDLFTLQLQEFDLTIKQAGNGREAVELLQI
                     HPDDFSLVLMDVHMPEMDGFTATRLIRKSEAGTGRHIPIVAITADAMVSSKKLCLEAG
                     MDDFLTKPVTLANLDKLFR"
     gene            complement(2619..3905)
                     /locus_tag="PLE39_12220"
     CDS             complement(2619..3905)
                     /locus_tag="PLE39_12220"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNE69142.1"
                     /translation="MIQKIKELLRSKIASASALLMVSMTVVNAGNYVYNLIMGRWLGP
                     SLFADLSLIVTLLLIVTFITAPIQMTTARYTAIHAADADVKTLASIRRFIWRIAAALG
                     LTLTVLFATFAPLLQSFFHTQSFLPFIIFGAALPFSLLQGVERGILQGRTHFKVLAIS
                     YQVEMWTRLFVSIILVALGLSVNGAVIGISLSFFATWLTARLAVRDLFTKEDIQTELR
                     KELFAFAVPVLVSQLGQILINNSDVILVKRFYESDQAGQYAALALIGRIVFFATWSVV
                     TTMFPIVAQKQKQGEPHSHLLWVSLGIVLGVSLPIVLLTLFVPEWIVKILFGSQYLGI
                     APLLWLYALATTLYALANVVINYRLSLGMGRETRFALAAGVAQVLGIIFFHQTLAQVV
                     YVQVVVMAVLFASLLWWNFSGRARQLPLIQVAKSNA"
     gene            complement(3902..4267)
                     /locus_tag="PLE39_12225"
     CDS             complement(3902..4267)
                     /locus_tag="PLE39_12225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE69143.1"
                     /translation="MARILIVDDEPNNQRILNYTLNKAGYETITAANGETALELLDQY
                     DADLAIVDVAMPVMDGLTLLKHIRSADKFEHLPVIILTGSGDDEERLRAEREGIQGFL
                     TKPSSSKMVLETVQNILKR"
     gene            complement(4269..4613)
                     /locus_tag="PLE39_12230"
     CDS             complement(4269..4613)
                     /locus_tag="PLE39_12230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE69144.1"
                     /translation="MTLEISISEQVIKIATIALNGRLDALEAPALRKRVDDYLANEFS
                     HFVFDLSDVAVLDSAGLAVLVSALKRARLKNGDVRLVWPKAEVASRILKLTKFDQVFV
                     SIERSEIVPRGF"
     gene            complement(4640..6550)
                     /locus_tag="PLE39_12235"
     CDS             complement(4640..6550)
                     /locus_tag="PLE39_12235"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE69145.1"
                     /translation="MLAEHLENHRKKFYSLASVWMRVGATCVELMEAGQVLATFPQEC
                     SDSSPALFVHARTSNLALRIHGIVDENCRPIAESMLDILSNLLTADLELENLTAALVE
                     TQDRLVAIYELSQASRRTLEIPALLDLLVQESTHLMNVDGGFAVLMEKGKKSVIHQVS
                     DAPLHHAHLEAAATLFRRDPTRHTFKESETLPPGLRNVMMVSLPVRDEVFAAFGVFNK
                     TGDFTSPDIKLAKAIAGYVGARLESAFLHKEALERARMETEMDIARQVQIAILPQSLP
                     KVTGLDIYATSTPALEVGGDFFDVIDRSEDSLVFAVGDVTGKGMPAALLMSMTHTVIK
                     SASRKMPFSHPHQVLNRLNHDLFSDFSNVGMFSTVFLGLLDRKNCTVTFSNAGHSPIF
                     YIPAGCEAHLLEAQDIPIGVLDIYDYTSQTIKFATGDIFVIASDGFPESRNSSDEMYG
                     YDRMKACLERSRNAPAEEMVSALLADVNAFSGSHLQDDDRTILVIKVNELRMQSESIN
                     IRSTYKEIRLPSERLRGLLASNQVTEEIINGCELALHELLTNLVDHAYEGNENGNITV
                     NITCDTDFVTIETRDEGKPANVDMAGIAMPDPTDLAEGGYGMAIIQMLMDDVKYKFED
                     SKNVWRLIKRIQ"
     gene            complement(6557..6889)
                     /locus_tag="PLE39_12240"
     CDS             complement(6557..6889)
                     /locus_tag="PLE39_12240"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE69146.1"
                     /translation="MEMIKVVNGPISVLQLKGRFDAHEVEPVNAWLQEQVNQGRVKVV
                     VNLEGVNFIDSTALSVLVRGMKNCREKSGNLNLCSLQQPVRVIFELTRLDKAFGIYAS
                     ETEAGKAF"
     gene            complement(6899..8104)
                     /locus_tag="PLE39_12245"
     CDS             complement(6899..8104)
                     /locus_tag="PLE39_12245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE69147.1"
                     /translation="MTTTNQMRIAIVTTHPPSKGSLNEYAFHFVRFLKTKVEYVQEII
                     LLVDELPEGESYSLGQKQDEVPIRCVPCWKFGALDNILRIRKAVKEVEPDIVLFNIQF
                     ASFADKKIPATLGLLTPWFLKVSGFKVAVLLHNIMETVNLKSAGFGSNPLIEAVIRLM
                     GNIVTRFLLAADLVAVTIPKYVEILESKYDATNVLLAPHGSFEEPTDTEPKPLPGGTP
                     TIMTFGKFGTYKTVDLLIESHAMLQTQHPDLRLVIAGTDSPNAAGYLKTMQEKYAHVK
                     NVEYTGYVAEEDVPRIFGEAWVVAFPYTSTTGSSGVLHQAGGFGKAVVLPKLGDLAEL
                     VLEEGYDGEFFEPTNSKSLADAIARVIDNPERQMEIGLRNYTAANGLPITEVVDWYLI
                     HFEELLKRK"
     gene            complement(8101..8865)
                     /locus_tag="PLE39_12250"
     CDS             complement(8101..8865)
                     /locus_tag="PLE39_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE69148.1"
                     /translation="MPYENYAQWKIVPISEEPKLSIVIPAYNERERIVATIGAIASHV
                     SDLGFPWEMFIADDGSKDDTVAIVEELGLVNLTVLKAPKNGGKGSAVQRGMLAARGKY
                     VLFADADNSTPIEEVSKLLNKLEHDGFDVAVGSRAAEGAEEGKKSLFRKILSGGLRWI
                     VKNIFRIGVRDTQCGFKMYTHEAAQKLHAKQTIMGFSFDLEILYLASKYGYKIAEVPV
                     SWIDAPGSKVDTRKEVQRFLRDLLKIKSNDLRGVYQ"
     gene            complement(8878..10044)
                     /locus_tag="PLE39_12255"
     CDS             complement(8878..10044)
                     /locus_tag="PLE39_12255"
                     /inference="COORDINATES: protein motif:HMM:NF016104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HNE69149.1"
                     /translation="MKKLPAWLLIPLLAFSLSRLLIFGAGILADTMLPTEEGHWIADE
                     NSPFLSMWTKWDSQYYVDIATDGYWFRPKQQSNVAFFPLYPMLMRVTSKLLGGNLILT
                     GVLLSNLAFLGALIFFYLLTEREFDSDSARRAVFYLAFFPTSFFFSSLYTESLFVLLS
                     VATMYYARTQRWVPATILGMLTAATRNLGILMWALVLWEWLRVQGWSFTAMHKKQTWL
                     NLWNGFKHNWFQVIIISLIPLGMLAYIYFLQVNFQRPLAFIETQAAWGRENIGPIAVL
                     QKNITALMEGEVNKGWLTRFWNVTTMLFFLALVPFIWFRLGEGYAIFVLIMLLVPSAS
                     AVGSIIRYVLTQFPAFMLMGWWGRREAVDRTFGMSFAVLLGVFVSIFVNWIFVA"
     gene            complement(10041..12563)
                     /locus_tag="PLE39_12260"
     CDS             complement(10041..12563)
                     /locus_tag="PLE39_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69150.1"
                     /translation="MKTFVYPYIEKIKGLQERLTAILDRLRTKIPGLPSNRILISAAG
                     LVFVAVFIFAALQTLLAPEQPVTTYTVPYTQEFDEVNLKRWFSDAGVWTIRESTLLQT
                     VGGEDAGQLHIPLTVPEDQPYHASVFITLKKDSKNVGLSFNAQYPNLLEEQHRVYISR
                     INPDTLELVAGYMDETGAFVTQAQVPLSINTTEFRLDLYVYSNTYLVQVNGQRLIENR
                     PLFYKNGMIGFYALGPVIFDTYKLTAAENQNPGDMIYTSDFDQDPGGAGWVPISGMWQ
                     IASKQMVQSDSAVNDAAIGYETSTFENYTLQTTFNHLSGQGAGVLFNMPSPYQINGAH
                     LVRYSDETDSLVWGYYDAKGVFNRQGFADVSAAGKAAHTLQIFSGDNSYDIFLDDEML
                     ARDVPLESIQGSVGLTTSRSSAGFTTVEVFPLFGTTASPVAALTQPSKNTPLPKASPT
                     QVLPTQASPTQTPAPEKTPTANADSVTPIATGDKNIVNGGSAPYHGVFDGKLSDAGWK
                     ILNGDWKFSNGNFVQTKVEGFDFATVYTKTAFKSFSIQIGLMHVEGSGGGIIFNLPYA
                     DRLNGATMVRYSDKRDNAVIWGYFDENGVYQNQGYADVPHAGTDRHTLRVVAGEASYG
                     IYVDERLIVQAVPYRTKQNFGYFGLLTSRSSVVYDEVLVDGVGAVFKGTYSNMDGFTD
                     QRVVSGKWNIETNKASQTVTDVSDYVWNTGVQASDYTVTSKITLPNTSDSGGGFIIHM
                     TERGTKNNSYIVRLKDGGKAIWWGTINAEGKFKGQGSAPLEKTSNTFVLKLVVKLDSM
                     TIYVNDKEIVKNVTLSSQEGWIGLLAYGGPVIFEDVKLDVTQ"
     gene            complement(12685..>13179)
                     /locus_tag="PLE39_12265"
     CDS             complement(12685..>13179)
                     /locus_tag="PLE39_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69151.1"
                     /translation="VPREAPFRIDVTLPADGEYRLLMRGAISAVDLEMTSKLFETPQR
                     ITLASDPSNLAFFDKRMVFSSKRSPLDVSAYTLEELGLLIPSDVVVVNYQYQFFDLGT
                     VTAGMGKYPIYFTKLNDAPMLVEGILLVPEEAYRSMSLPPNVTVVPANELCCGSVIIQ
                     GEEP"
     assembly_gap    13180..13279
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13280..15453)
                     /locus_tag="PLE39_12270"
     CDS             complement(<13280..15453)
                     /locus_tag="PLE39_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69152.1"
                     /translation="MKSARNLSAADDQPGISEKQPRFALLSFRALTIRQISMIVGICY
                     FLISFFVFRHVIANIPLVLSGQAVINGDELVPFFNPNSQLIDQAAGKFNQLTNGYEFR
                     VRYSILTTWFRYYKVLPFAIVLVIPTITYLAYLATASLLRKALPQFKPENIYQLTAAP
                     ILMIFLIMMYAKITHFYTLILGFSLCLISITLMTYGLVFPQPQPYKPILAACVITLFN
                     PAVHYLILFALFMAMTVFTLIVMDGIAAIKNGSWRNIYRPRVWWGGVKTLWKDWRRLF
                     VEVRFWRCVGAFVMLGVGTLLPYGLFVKFYALRGVPNLSETVPADFYFIQDASISLGH
                     VLALDMAGIMDKELTGDYLAKVPRWSNMIYTLLMFIPLVVKRARDDIFHSEPLKALSA
                     VLYANTIFTMWATLGYSTAPWVPTFHRTIAFISLKANGLQSGIGDLVVKLMGTIVQVL
                     RFPHRFELVTFMMACILVSISMIWMHHVFMEKGFGELVWVLTGKRQGAAKKSLKQNQA
                     RAGAEAQRFIPLTMVLLFMVPLFLNSSYRLVFFSGDFNEFLTPYPVGPLKEVKEALLQ
                     LPPGKVVVLPPTETAKVIVDINGVEHKFIDKFHIYYLDLPSYYYGLTGDSDNKHEFFL
                     LLRALYYQQPWWINIARDLNLRYIVVNKELVANTVGGQEYLREVEKILIPELDRRDAY
                     LKKLMENESFVLYEFTDLPKAERVPLYLDVDWNKFIQILSSNL"
     gene            complement(15422..16558)
                     /locus_tag="PLE39_12275"
     CDS             complement(15422..16558)
                     /locus_tag="PLE39_12275"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNE69153.1"
                     /translation="MKILFLGTHGQFNIGDELLLETFLAQLGIEHQYAVNSYDPTFTQ
                     NAMRPKFKVESFHTTRELPRFLKYLLTSDLLFFGGGSIIKELYASVGRNPYSTLLMIL
                     ATVTFAKQIARKRVVMSNIGVGPIMTLRGEQLARWILNQVDILSVRDEKSLQTALKLG
                     LAPSRVMLVPDAVFANPPEVFVSHPEIPPPSDKTRIALNLNYDIENPNAWEAFLTNLA
                     TALKQLGETHALEIHALPMQSKFKANDDLSVLKDFTKRIPDIPCILHDPQTAQEAAQI
                     ILGMDMVLAERLHTLVISSVLGKPFFGLVYDVKVKELIDYLGMSEHSLNINQSFTAEE
                     LVNGINCVLDQRETISTHLLKRSTSLREQLGKYFDEIRQKFIGR"
     gene            complement(16740..17351)
                     /locus_tag="PLE39_12280"
     CDS             complement(16740..17351)
                     /locus_tag="PLE39_12280"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNE69154.1"
                     /translation="MMLAEILCESSRHHDHLCPRQVLGVRMGLYAGELLGLQLPRADK
                     RLLVIAETDGCALDGISAATGCRVGNRTLRVMDFGKVAATFIDVSTETCIRIAPSVQA
                     RSLALAYSPNSTSRWDAMLSAYQSMPVMDLLAFQRVQLDTPLAQILSVPGKKVQCEVC
                     GEEIMNEREVLKNEMILCRSCAGDGYYRYPVDSLSVEQLEQKG"
     gene            complement(17348..>18457)
                     /locus_tag="PLE39_12285"
     CDS             complement(17348..>18457)
                     /locus_tag="PLE39_12285"
                     /inference="COORDINATES: protein motif:HMM:NF021539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HNE69155.1"
                     /translation="GSGFFIQKENPMAVKKSTKAKSKPKQEEPIPVEEPVWTYRGYRL
                     KTSEFVTAMVHFFRAEIQRANVWRTRLDTTTNWAVVVTGATLSIAFSQSNVHHSVVLL
                     NTLLVLWFLFIEARRYRYYELWSYRVRLMETDFYAAMLVPPFHPSPEWAENLAENLLT
                     PSFPISMWEAFGRRLRRNYYWIFLILLASWVAKIYLFPEAPASMSEFVVRSSVGPVPG
                     EVMIGLGISFYTFLAIFAIATINMTKATGEILPRFGDESAPAAPSMEGKHTGLRALLV
                     PRRRRRQLLALIITDKADSVSGRIMTDLKRGVTSMLGKGMYTGKDRSILMCALTITEV
                     HNLKSAVAKEDPKAVVIVSPAQEVFGGGFAPLEEK"
BASE COUNT         4890 a         4793 c         4322 g         4352 t
ORIGIN      
        1 tcctaaataa tttatccagg ttggcaagcg taacaggttt ggtgaggaaa tcatccatgc
       61 ctgcttcgag gcacagcttt ttactgctca ccatggcatc agcggtaatt gcaacgatgg
      121 gaatatgcct tcccgttcct gcttcgctct tgcggatgag acgggtggcg gtgaaaccgt
      181 ccatttcggg catgtgtaca tccatcagaa ccagcgaaaa gtcatcaggg tgtatttgga
      241 gcaactcaac cgcctccctg ccgttcccag cctgttttat ggtgaggtca aattcctgta
      301 actggagagt gaacaggtca cgattaacga ggttgtcttc aacgatcaag atcggttttc
      361 tgctcgaata attcaggtat ttcttttgca tcccacggga agaaaactct gtttcttcct
      421 gatcctttgg ggcagtggta tacaagggca ggctaaacca aaacgtagac cctgtacctt
      481 caaaagattc aaatccaatc tcaccatgca tcaaatccac caaacgcctg gaaatggcca
      541 ggccaaggcc cgttccaccg tgtttacggg tgttcgagcc atcagcctgg gtgaaaggct
      601 caaaaagttt acttcgcatc atatcagaga cgccaattcc attatcctgc acggtaaagg
      661 tggtcatcaa cgtgttctgg ttgatgcttg ttcccgaaat attgatgaaa acgcagccat
      721 tcttctcggt aaatttgatg gcattgctga caaaatttcc gaggatctga cgaatcctgc
      781 cgccatcacc aacgagggtc tcaggaatgg atgcggtcgc catcatggag agagtgatgt
      841 tcttctcctg cgcccgcggc tggaatagtt tgatcagatc acgggtcatc tctacagggt
      901 taaaaacgga tggaatgatg gaaagtttgc ccgcttcgat cttggaaaaa tcaaggatat
      961 cgttcagaat ggcaagcagg ccttgcgtgg aggaatcgat ggtctgcgca aactcccgtt
     1021 gctcgtcatt caaatctgtg ctgaggagca tttcggtcat gcccataatg gcattcatcg
     1081 gagttcggat ctcgtggctc atggtggcca ggaaggaact tttgagatac gaagcttcca
     1141 aagcatggtc gcgggcatct gccagatcaa tattactttt ttcgatggcg gtggcataat
     1201 cacggagcag gttttccatc tgccgccgct ccgtcagatc ggtgatcaca ttgatcgtcc
     1261 cagcgaactg cccatccaca acatgaggca cgctggtgac aagggcgtac acctcatcgc
     1321 catttctacc gcgcaatcgt atttcaagcg tggtcacctc acctacattg cgtttcgaca
     1381 acaccgtttt aaccaactca tggtcttcag gaaaagtgac atcaaaggcg gtcaacccgg
     1441 ccagttgctc cgtttcgtat cccaatattt tcgcagagta tgggtttgca aacgtgaagt
     1501 ggccttcttc atcgatcacg gtcaacccct gccccatttg agtggtaagg gtttgggcaa
     1561 agtcacgttg attttgcagt tctccgcctg tcttcttact ttcagttacg tctgtgccga
     1621 tcccgatcaa aaggtcacgc ggaccttttc ggtcggccag catcaaccaa cgccagtcac
     1681 catttttatg gcgtacccga tgttcatacg gcttgtcatc cacctgacgc agcccggcag
     1741 cggccttgct aatgtaatcc cgttccgcca gcacatcccc cccggagatc cgctccaatt
     1801 cccaccagcc atttccgatc agttcttcgg gttcataccc aaggatctcc ttcaccgatg
     1861 ggctgatata caccacctcg gcattgctgt tagccaccag gaccaggttt ccgatcgaac
     1921 gcagaatttc atcggaaagc gcaagtctat tcttttcggc ctcatattct gtgatatcca
     1981 gagcgtacag ggtgacatac cccatttcgg gtgtgggatt aaagtgacag gaatataacc
     2041 tgcggccatg ttgaatgacc ctttgaatat gtgtttttct tttgagagcc tgctcaaaaa
     2101 agcccaccca ctctgagggg acctgtcccg taaccaccat tccactacgc cttaaaagcc
     2161 gtttaccgct tttgttcgca aaaaggattc ttccatctct gccaatgcga atctcagcat
     2221 tggggttttc ccgtgcgtcc gtgatgggca gatcggtcag gttttcttcc tgcccgtcaa
     2281 tcaaggagtc tctcaaagag ataagcgaaa caggttgatc attgagactc cccttcccaa
     2341 tggaggccat tacaaggaat tcataaccac ttttatgcag aacccatacc cgatgagatt
     2401 ttacgatttt tcgattttca acgttagtat tcagcagcaa caaaaccaga tctttacgat
     2461 tttttgcgtt cttcttcggc aggaggtttt ccacggtcat tgtttgaaat tcatccatag
     2521 aatagccgaa catctccgca gcaaattgat tcgcgcggat gaccctgcca gattcatcaa
     2581 aggcgatcag tgcatccatc gccacggaaa agaaaagatc aagcattgct tttagccacc
     2641 tgtatcaaag gcagttgtct ggcgcggccg ctaaaattcc accacaacaa actggcaaac
     2701 aaaaccgcca tcaccaccac ctgcacataa acaacctgcg caagtgtctg atgaaagaag
     2761 atgatcccaa gcacctgtgc cacgcccgcc gcaagcgcaa agcgcgtttc tcttcccatg
     2821 ccaagcgata acctgtaatt gatgaccacg ttcgccagcg catacaaggt ggtggccagc
     2881 gcatacagcc aaagcaacgg tgcaatgcca aggtattgac tgccgaacaa gatcttcacg
     2941 atccactcgg gcacgaacaa agtcagtaaa acaatgggca acgacacgcc caacacaatg
     3001 ccaagcgaaa cccaaagcaa atgactgtga ggctccccct gtttttgctt ttgcgccacg
     3061 atcgggaaca tggtggtcac cacagaccag gttgcaaaaa acacaatacg tccgatcaaa
     3121 gccagtgcgg catactgccc agcctggtct gattcataga agcgcttcac caggatcaca
     3181 tcactattat tgatcaagat ctgtcccaac tgcgaaacca gcacaggcac ggcaaaggcg
     3241 aacaactctt tgcgtaattc ggtctggata tcttcctttg tgaaaagatc tcgcacagcc
     3301 agccgcgctg tgagccatgt ggcgaagaac gaaagactga tgccaatgac cgccccattg
     3361 acggataatc ccagcgccac caatatgatg ctgacaaaca ggcgggtcca catctcaacc
     3421 tgatagctga tcgctaaaac cttgaaatgg gtccgcccct gaagaattcc acgctccaca
     3481 ccctgcagca aactaaatgg cagcgccgcc ccaaaaatga tgaagggtaa aaatgattgc
     3541 gtgtgaaaaa aactttgcaa caacggcgca aaagtggcga ataaaaccgt cagcgtaaga
     3601 ccaagcgcgg cggcgatgcg ccagatgaac cgtcgaatcg aagcgagggt cttaacatcc
     3661 gcatccgcag catggatggc cgtgtaacgg gcggtcgtca tctgtatcgg agcagtgatg
     3721 aatgtgacaa tgagcaacag ggtgacgatc aacgaaaggt cggcaaaaag cgacggcccc
     3781 aaccagcgcc ccatgatgag gttgtatacg tagttccccg cattgaccac cgtcatgctg
     3841 accatgagca aagcactggc cgatgcgatc tttgatcgta gaagttcttt aattttctgt
     3901 atcatcgttt caaaatattt tgcaccgttt caagcaccat ctttgagctg gaaggcttgg
     3961 tcagaaaacc ctgaatgcct tcacgctccg cccgcagacg ttcctcatca tcgccgctgc
     4021 ctgtgaggat aatgacaggc agatgctcaa atttatctgc agagcgaata tgcttgagca
     4081 gggtaagacc atccatgaca ggcatggcca catctacaat ggcgaggtct gcatcgtact
     4141 ggtccagtaa ctccagggcg gtttctccat tggcggcggt aatggtttcg tagccagctt
     4201 tgttcaaggt gtagtttagg atgcgctggt tgttgggttc atcatcaacg atcaggatac
     4261 gtgccatgct aaaaccctcg cggtacgatc tcagaacgtt caatggatac gaacacctgg
     4321 tcaaatttgg tcagcttcaa aatgcgtgat gccacctcgg cctttggcca caccagacgc
     4381 acatcgccat tcttcaagcg ggcgcgcttc aacgcgctga ccagaacagc cagccccgcc
     4441 gagtccagca cggctacatc cgaaaggtcg aacacaaagt gtgagaattc gtttgccagg
     4501 taatcatcca cccttttccg cagggcgggt gcctccaagg catccaacct gccattcaga
     4561 gcgatcgtgg cgatcttgat aacctgttca ctgatgctaa tttcaagtgt catgggaagt
     4621 tcctctttct atttattgct cattgaatac gtttgatcag ccgccaaaca ttctttgaat
     4681 cttcaaattt atatttgacg tcatccatca gcatttggat gatagccatg ccatacccgc
     4741 cttcggccaa atcagttgga tctggcatgg caatccctgc catgtctaca ttggcaggct
     4801 tgccctcatc acgggtctcg atcgtgacga aatcggtatc acaagtgatg ttgacggtga
     4861 tatttccatt ttcattgcct tcgtaggcat ggtcgaccag attggtgagg agttcatgca
     4921 gggcgagttc acagccattg atgatctctt ccgtcacctg gttggaagcc agcaagcctc
     4981 gcaatcgttc cgaaggcagg cgaatttcct tgtaagtgga acggatattg atcgattcag
     5041 actgcattct taattcattc accttgatca ctagaatggt tcggtcatca tcctgaagat
     5101 gcgacccaga aaaggcgttg acatccgcca aaagtgcgga aaccatttct tcagcagggg
     5161 cattcctcga acgctccagg caggccttca tgcggtcata gccatacatt tcatctgaag
     5221 aattacgcga ttcggggaaa ccatctgaag caatgacaaa aatatcgcct gtcgcaaatt
     5281 tgatggtttg ggatgtgtaa tcgtaaatat ccagcacccc gatgggaata tcctgggctt
     5341 ccagtaaatg ggcttcacac cccgcgggaa tgtagaaaat cggactatgc cccgcattgc
     5401 taaacgtcac ggtgcaattc ttgcgatcca acaggcccag aaaaacggtg gagaacatgc
     5461 ccacattgga aaaatcgctg aacagatcgt ggttcaacct gttcaatacc tgatgcgggt
     5521 gtgagaacgg cattttgcgc gatgcagatt tgatgaccgt atgcgtcata ctcatcaaca
     5581 gcgcagcagg catacctttg cctgtcacat ctcccacggc aaagaccagc gaatcttccg
     5641 agcgatcaat gacatcgaag aaatcgccgc ctacctcgag tgcaggtgtg gaggtggcat
     5701 aaatatcgag tcctgtcacc tttggcaggg attgtggaag gatggcgatt tgcacctggc
     5761 gggcaatatc catttccgtt tccatgcggg cgcgttcaag ggcctcctta tgcaggaagg
     5821 cactttcaag ccgcgcaccc acatagcctg caatggcttt cgccaactta atatctggag
     5881 aagtaaaatc acccgtttta ttgaaaaccc caaatgcggc aaagacctca tcacgcacag
     5941 gcaacgaaac catcattaca tttctcaaac ctgggggcag ggtttcagat tctttaaaag
     6001 tatggcgtgt tgggtcacgg cggaacaagg tcgccgccgc ttccaaatgc gcatggtgca
     6061 ggggggcatc ggaaacctga tggatgacag acttcttgcc cttctccatc aacaccgcaa
     6121 aaccgccatc cacattcatc aggtgagttg attcttgcac cagtagatcg agcagggcgg
     6181 gaatttccaa ggtgcggcgg gaagcctgcg acaattcata gatcgccacc agcctgtctt
     6241 gagtctccac cagggcagca gtgagatttt ccagttccag gtcagccgtg agcagattgg
     6301 aaaggatatc aagcatcgat tcagcgatcg gcctgcaatt ctcgtccacg attccgtgaa
     6361 ttcgcagagc caggttcgag gtccgcgcat gaacgaagag ggcgggcgaa gaatcactgc
     6421 actcctgcgg aaaagtcgcc agcacctgac cagcttccat caattccacg caagttgcac
     6481 ccacccgcat ccacacagat gcaagcgaat aaaacttttt gcgatggttt tccaagtgtt
     6541 cagccagcat ggtttcctaa aatgccttgc ctgcttcagt ttcagatgcg taaattccaa
     6601 acgccttgtc gagacgggtt aattcaaaga tcacacgcac aggctgctgc agcgaacaca
     6661 gattcaaatt acccgacttt tcacggcagt tcttcattcc gcgcaccagc acagaaagcg
     6721 cagtggaatc tataaaattg actccctcca gattgacaac caccttcacc ctgccctgat
     6781 tcacctgttc ctgcagccag gcgttgacag gctccacttc atgcgcatca aaacgacctt
     6841 tgagctgcaa caccgaaatc ggtccattca ccaccttgat catttccata aaaactcctt
     6901 atttcctttt caataattct tcgaaatgga tgagatacca atcaaccact tcagtgatcg
     6961 gcaggccgtt ggcagctgta tagtttcgca gcccgatctc catttgacgc tcggggttat
     7021 caatcacgcg tgcaattgca tcggcaaggc tctttgaatt cgtcggttca aaaaattcgc
     7081 cgtcgtatcc ttcttcaagc accaattcgg caaggtcgcc caattttgga agcacgaccg
     7141 cctttccaaa accacccgct tgatgcaaca cacccgaact cccggttgtg gatgtgtaag
     7201 gaaacgccac gacccatgct tcaccaaaaa tacgtggaac atcctcttca gccacataac
     7261 ccgtgtactc cacatttttc acatgggcat atttttcctg catggtcttc aaatatcccg
     7321 ctgcattcgg gctgtcggtg cctgcaatga ccaacctcag atcagggtgt tgtgtttgca
     7381 acatggcatg cgactcgatc agcaggtcca cggttttgta ggtgccaaat tttccaaagg
     7441 tcataatggt gggcgtaccg cctggcaggg gcttcggctc tgtatcggtg ggttcttcaa
     7501 acgatccatg cggcgccagc aacacattcg ttgcatcgta tttgctttca agaatctcaa
     7561 cgtacttggg aatggtcacc gccaccagat cggcagccag caaaaagcgg gtgacgatat
     7621 ttcccatcaa ccgaatcacc gcctcgatca gcgggttgct gccaaacccc gccgatttca
     7681 aattcaccgt ctccataatg ttatggagta gaaccgccac cttaaaacca gacaccttca
     7741 aaaaccacgg agtcagcaag cccagcgtgg cgggaatttt tttatcggca aaagatgcaa
     7801 attgaatatt gaacaaaacg atatctggct caacttcttt taccgccttg cggatacgga
     7861 ggatgttatc caacgcgccg aacttccaac acggcacgca tcgaatcgga acctcgtcct
     7921 gcttctgtcc gagtgagtaa gattcaccct caggcagttc gtccaccaac aaaatgatct
     7981 cctgcacata ctccaccttc gtcttcaaaa aacggacgaa atgaaaagca tattcgttca
     8041 aagagccctt tgaaggtgga tgggtggtga caatggcgat tcgcatttga tttgtcgtcg
     8101 tcattggtac accccgcgca ggtcattgga tttgatcttc aacaggtcac gcaggaaacg
     8161 ctgtacttcc ttgcgtgtgt ccactttgga gcctggggca tcgatccacg aaacagggac
     8221 ctcggcaatt ttgtatccgt acttggatgc cagatataaa atttcaaggt caaaggaaaa
     8281 gcccatgatg gtctgcttgg catggagctt ttgggcggct tcatgggtgt acattttgaa
     8341 tccgcactgc gtatcccgca caccgatacg gaaaatgttt ttcacgatcc agcgcaatcc
     8401 cccgctgagg atctttctga agagcgattt cttgccttct tcggcacctt ccgcggcccg
     8461 tgagccaaca gccacatcga agccgtcatg ctcaagtttg ttgagcagtt ttgaaacttc
     8521 ttcaatgggg gtggaattat cggcatcggc aaaaaggaca tattttccgc gggcagccag
     8581 catcccccgt tgaaccgcac tgcccttgcc gccatttttc ggggctttga gaacggtcaa
     8641 attcaccagg ccaagttctt ccacaatggc cacggtatca tcctttgagc cgtcatctgc
     8701 gatgaacatc tcccacggaa agccgagatc tgagacatgt gaagcgatcg cgcctatcgt
     8761 tgcaacgatc cgctcgcgct cgttgtaggc tgggatgaca atggacagct tcggctcctc
     8821 ggaaatggga acgattttcc attgggcata attttcataa ggcattcgaa cctcctttta
     8881 cgcaacaaag atccaattga cgaaaataga aacaaaaaca ccgagcaaca cggcaaacga
     8941 catgccgaaa gtacggtcta cggcttcgcg gcgcccccac caccccatca acatgaaagc
     9001 ggggaattgt gtcagcacat agcggatgat gctgcccaca gcactggctg aagggaccag
     9061 cagcatgatc aaaacaaaaa tggcataacc ttcccccaat ctgaaccaaa tgaaagggac
     9121 cagggcaagg aagaacagca tggtggtgac attccagaaa cgggtcagcc aacccttgtt
     9181 cacttcgcct tccatcaagg cagtgatatt tttctgaagc acggcgatcg ggccgatatt
     9241 ttcacgcccc cacgcggcct gcgtttcgat aaaagccagc ggccgttgga agttcacctg
     9301 aaggaagtaa atgtaggcaa gcattcccaa tggaattagt gaaataatga tgacttgaaa
     9361 ccagttgtgt ttaaatccat tccacaggtt gagccaggtt tgctttttgt gcatggcggt
     9421 gaaagaccaa ccctgcaccc gcagccactc ccacagaacc aaagcccaca tgagaatgcc
     9481 aaggttgcgc gtggcggcgg tcagcatgcc gaggatggtg gctggcaccc agcgttgtgt
     9541 tcgggcgtaa tacatggttg ccacagaaag aagcacaaaa agactctcgg tatacagtga
     9601 ggagaaaaaa aacgaggtgg ggaaaaatgc caggtagaac accgcacgcc gggctgagtc
     9661 agaatcgaat tcgcgttcag tcaacaggta gaaaaatatc aaggcgccaa ggaaagccag
     9721 gttgctgagc agaaccccag tcagaatcag gttgccgcct aacagtttcg aagtgacacg
     9781 catcaacatg gggtatagcg ggaagaaggc tacattgctt tgctgcttgg gacgaaacca
     9841 gtagccgtcg gtggcgatat ccacataata ttggctgtcc cattttgtcc acatggaaag
     9901 gaagggactg ttctcatcgg cgatccaatg accttcttca gtgggcagca tggtatcggc
     9961 aaggatgcct gctccaaaga taagcaggcg gctgagtgaa aaagcaagca ggggaatgag
    10021 cagccaggct ggaagtttct tcattgcgtc acatccagtt tcacatcttc aaaaatgacg
    10081 gggccgccat aggccaacag cccgatccat ccctcctgcg aggacagagt cacattcttg
    10141 acgatctctt tatcgttgac atatatggtc atggaatcga gtttgaccac caatttcaag
    10201 acaaaggtat tgctggtctt ttcgagcggt gccgagccct gtcctttgaa ttttccttcg
    10261 gcattgatcg tgccccacca aatggctttg ccgccatctt ttaagcggac aatgtaagag
    10321 ttgttctttg tgcctcgctc ggtcatgtgg atgatgaacc cgccgcctga atcactggtg
    10381 ttgggtaaag tgatctttga ggtcaccgtg taatcggaag cctgtacgcc tgtattccag
    10441 acatagtctg aaacatcagt gacggtttga gatgccttgt tggtttcaat gttccacttg
    10501 ccactgacaa cacgctggtc ggtaaaaccg tccatgttgc tgtaagtacc cttgaatact
    10561 gcgccaaccc catccaccaa cacttcgtca tacactacac tggaacggga ggtcagcaga
    10621 ccaaaataac caaagttctg ttttgttctg tagggtaccg cttgcacaat gagccgttca
    10681 tccacataaa tgccataaga ggcttcgcct gccaccacac gaagggtatg gcggtcggtg
    10741 cctgcatgtg gaacatcggc atatccttga ttttgataaa cgccattctc atcgaaatag
    10801 ccccagatca cggcattatc gcgtttatct gaataccgca ccatggtggc gccattcaaa
    10861 cggtctgcat aaggcaggtt gaagatgatg cccccgccgc ttccttccac atgcatcaga
    10921 ccaatttgaa tactaaaact cttaaaggca gttttggtat ataccgtggc aaaatcaaag
    10981 ccctccacct tggtctgcac gaaattacca tttgagaatt tccaatctcc attgaggatc
    11041 ttccaaccag catccgacag cttgccgtca aatacaccat gatagggagc gctgccacca
    11101 ttgacaatgt tcttgtcacc cgtggcaatt ggtgtgacgg aatccgcatt cgcggtgggt
    11161 gttttttcag gagccggggt ttgggtgggt gaggcttgtg ttggtaaaac ttgtgtaggt
    11221 gatgcctttg gaagcggagt attttttgaa ggctgggtca gtgcggcaac agggctggcc
    11281 gtggtaccaa acagcggaaa cacttcaacg gtggtaaagc cagccgaaga acgggatgtg
    11341 gtcagaccga ccgagccttg aatgctttca agaggcacat cgcgcgccaa catttcatcg
    11401 tccagaaaga tgtcgtaact gttatcgccg gagaatattt gcagggtgtg ggcagccttc
    11461 cctgctgctg aaacgtcggc aaatccctga cggttgaaca cacctttcgc gtcgtaatat
    11521 ccccaaacga gtgaatcggt ctcatctgaa tatcgaacaa gatgtgcccc gttaatttga
    11581 tacggcgagg gcatgttaaa tagtacaccc gctccctgcc cggaaagatg attaaaggtg
    11641 gtctgcaggg tgtaattttc aaaagtactg gtttcatacc caatggcagc atcgttaacc
    11701 gctgaatcgc tttgaaccat ttgcttcgag gcgatctgcc acatgccact gatgggaacc
    11761 caacccgccc cgcccggatc ttggtcaaaa tcgctggtat agatcatgtc gccggggttt
    11821 tgattttcgg cagcggtcaa cttgtaggta tcaaaaataa ccggacccag cgcataaaaa
    11881 ccgatcatgc cgttcttata aaataacggg cggttttcga tcagccgctg gccgttgacc
    11941 tgaacaagat aagtattgct atagacatac aggtccagtc gaaactcggt ggtattgatg
    12001 ctgagtggaa cctgagcctg ggttacgaag gcaccggtct catccatgta accagcaacg
    12061 agttcgagcg tatccgggtt gatgcggctg atatacacac gatgctgttc ttcaagcagg
    12121 ttgggatatt gtgcgttaaa agataacccg acattcttgc tgtccttttt gagcgtaata
    12181 aacacactgg catggtaggg ctggtcttcc ggcacagtaa gcggaatatg caactgtcca
    12241 gcgtcctccc ctccaaccgt ctgcagaagt gtgctttcgc gaatcgtcca caccccggca
    12301 tcggaaaacc agcgtttgag gttcacttcg tcgaattcct gggtgtaggg aaccgtataa
    12361 gtagtgacgg gttgctccgg tgcaagcagg gtttgcaagg cggcaaaaat aaaaacggcc
    12421 acaaaaacaa gcccggcagc tgaaatcaaa atacgattgg atggcaagcc gggaatcttt
    12481 gtccgcagcc tgtcgagaat ggctgttaac ctttcttgaa ggccctttat tttttcaatg
    12541 taagggtaca caaaagtttt catcgtttat ttgtttttct tttccattct aagtccacca
    12601 cttttgaaaa tgcgtttcag agtcattttt aaaaaacttg ctgttttgaa aacacaatta
    12661 acattttttt tatgcctaaa tctctcatgg ttcttcgccc tgaatgatga ccgacccgca
    12721 gcagagctcg tttgcaggca ccaccgttac attaggcggc agggacatgg agcggtaagc
    12781 ctcctctgga accagcagaa tcccctccac caacatgggc gcatcgttga gttttgtaaa
    12841 atagatcggg tatttcccca taccagccgt aacagtgccc agatcgaaaa actgatattg
    12901 atagttcacc accaccacat ccgaaggaat cagcaggcca agttcctcca atgtgtacgc
    12961 gctcacatcc aatgggctgc gtttggacga aaataccatg cgcttatcga aaaaggccaa
    13021 attcgacggg tctgaagcca gagtgatccg ctggggtgtt tcgaacaatt tcgaggtcat
    13081 ttccaaatcc acagccgaga tcgcaccacg catcaacaaa cggtattcac cgtctgcagg
    13141 cagggtcaca tcaatacgaa atggcgcctc gcgcggcacn nnnnnnnnnn nnnnnnnnnn
    13201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13261 nnnnnnnnnn nnnnnnnnna ggttcgaaga aaggatctga atgaacttgt tccaatcgac
    13321 atccaaatac aaaggcacac gctccgcctt tggcagatcg gtaaattcat acagcacaaa
    13381 gctttcgttt tccatcaact ttttcaaata cgcatcgcgc cgatccaatt cagggatcag
    13441 gatcttttcc acttcacgca gatattcctg cccgcccacg gtgttggcta ccaattcctt
    13501 gttcactacg atataacgaa ggttcaagtc acgcgcaata ttgatccacc acggctgctg
    13561 gtaatacaag gcacgcagca acaaaaagaa ttcatgcttg ttatccgaat cacccgtcaa
    13621 accatagtaa taacttggca ggtcaagata atagatatgg aatttatcga tgaacttatg
    13681 ttcaacgccg tttatatcca cgatcacttt ggcagtctca gtgggcggca ataccaccac
    13741 cttgccaggc ggcaattgca acaaagcctc cttcacttct ttcaaaggcc ccaccggata
    13801 tggcgttaga aattcgttga aatcacctga aaagaaaacc aggcgatacg atgaattcaa
    13861 gaacagcggg accatgaaaa gcaaaaccat ggtcaatggg atgaaacgct gcgcttcagc
    13921 ccccgcccgg gcctgattct gtttcaagga ttttttcgcc gccccttgtc ttttccccgt
    13981 caacacccaa accaattcac cgaacccttt ttccataaaa acatgatgca tccaaatcat
    14041 gctgatcgaa accaatatac aggccatcat gaatgtgacc agttcaaaac ggtgcggaaa
    14101 acgcagcacc tgcacgatcg tccccatcag ctttaccacc agatcgccaa tgcccgactg
    14161 cagcccattg gctttcaggc tgatgaatgc aatggtacgg tgaaaggtcg gcacccacgg
    14221 cgcagtggaa taacccagcg tagcccacat cgtaaagatc gtattggcat ataaaacagc
    14281 cgaaagagcc ttcaacggtt ctgaatggaa gatgtcatcg cgggcgcgtt tcaccaccag
    14341 cggaatgaac atcaacaacg tatagatcat gttcgaccag cgcggaactt tggcaagata
    14401 atcacctgtc agttccttgt ccataatacc tgccatatcc agcgccaaga catgccccaa
    14461 tgaaatggat gcatcctgaa tgaaataaaa atcggcgggt actgtttcag aaagattcgg
    14521 cacgccacgc agggcataga acttcacaaa caagccgtat ggcagcagtg tccccacccc
    14581 cagcatgaca aaggcgccga cacaccgcca aaagcggact tccacaaaca gcctgcgcca
    14641 atccttccac aaagtcttca ctccgcccca ccacacccgc ggacgataga tattgcgcca
    14701 cgaaccattt ttgatcgccg caatcccatc catgacgatc aatgtaaaga cggtcattgc
    14761 catgaaaagc gcaaaaagga tcaggtaatg tactgcagga ttgaaaagtg taatgacaca
    14821 ggccgccagg atcggtttat atggctgtgg ttgaggaaag accaagccat aggtcatcag
    14881 cgtgatcgag atcaggcaaa gggaaaatcc caggatcagg gtgtagaaat gagtgatctt
    14941 cgcatacatc atgatcaaaa agatcatcaa gatgggtgcg gcggtcaatt gatagatgtt
    15001 ttcaggcttg aactgcggca gggctttcct caaaagcgaa gcagttgcaa ggtatgccag
    15061 atatgtgatc gtgggaatga ccagaacaat ggcaaagggc aaaaccttgt aatatcggaa
    15121 ccaggtggtc aaaatggaat atcggacacg gaattcgtag ccattggtca actggttgaa
    15181 tttccctgcc gcctgatcga tcaactggct gtttgggttg aagaacggca ccaactcatc
    15241 accgttgatc acggcctgtc cacttagaac gaggggaata ttggcaatga catggcgaaa
    15301 aacaaagaaa gaaatcaaaa aatagcagat tcccacaatc atggaaatct gccgaatcgt
    15361 tagagcacga aatgaaagca gcgcaaatcg cggttgtttt tcagaaatgc ctggctggtc
    15421 gtcagcggcc gataaatttc tggcggattt catcgaaata ctttcccaat tgctcacgaa
    15481 ggctggtgct tctcttcaac agatgagtgc tgatcgtttc acgttgatcc aagacacagt
    15541 taattccgtt aaccagttct tctgcggtga aagactggtt gatattcagt gaatgctccg
    15601 acatgccaag gtaatcgatc aactccttca ctttgacatc gtagaccaaa ccgaaaaatg
    15661 gttttcccaa aacggatgaa attaccagag tatgcaggcg ttccgcgaga accatgtcca
    15721 tgcctagaat gatctgggca gcctcctgtg ccgtttgcgg atcatggaga atacagggaa
    15781 tatcaggaat gcgctttgtg aaatctttta gcacggagag atcgtcattg gccttgaatt
    15841 tgctttgcat gggcagcgca tgaatctcca gcgcgtgagt ttcacccaat tgtttcaacg
    15901 cagtagcgag attcgtgaga aaggcttccc atgcatttgg attttcaata tcgtagttca
    15961 agttgagggc tatgcgggtc ttatcggatg ggggtggaat ttcagggtga gagacaaaga
    16021 cctcaggcgg gttggcaaaa acagcgtcag ggacgagcat taccctgctc ggggccagac
    16081 caagtttcag cgcggtttga agcgactttt cgtcacggac ggaaagaata tccacttgat
    16141 tcaatatcca gcgggcaagc tgttcgccgc gcagggtcat gatcggcccc actccaatat
    16201 tgctcatcac cacccgcttg cgtgcgatct gctttgcaaa tgtaaccgtg gcaagaatca
    16261 tcaaaagtgt ggagtatgga ttgcgcccca cgctggcata gagttccttg atgatgctgc
    16321 cgccgccgaa aaacaaaagg tcactggtga gcagatattt caaaaaacgc ggaagctcgc
    16381 gcgtggtgtg gaatgactca actttgaatt ttgggcgcat ggcattttgc gtgaaggttg
    16441 ggtcgtagga attaacggca tattgatgtt caataccgag ttgggcaagg aatgtttcca
    16501 gcaatagttc atcgccaata ttgaactgcc catgtgtgcc aagaaaaagt atcttcattg
    16561 ggtccgcctt tgtggttgaa ttcataataa tcactttcac aaaaacaaaa tctgacagga
    16621 tgattgcaaa cgcaaaaaac ttgccaattt gcaaggcatt ttgaattggc ataaacaaaa
    16681 aggctgcaga gtttctgcag ccttgaaaaa acttaagtag cactcactta agatcttgtt
    16741 tatccttttt gttcaagctg ttcaacggaa agtgagtcaa caggatagcg ataatatcca
    16801 tcccccgcac aagaacggca caggatcatt tcatttttca agacctcacg ctcgttcatg
    16861 atctcctctc cacaaacctc gcattgcacc tttttacctg gcacactcaa gatctgcgca
    16921 agtggagtat ccaattgcac ccgctggaat gcaagcagat ccatcaccgg catggattga
    16981 tatgccgaca acatcgcatc ccaacggctg gttgagtttg gagaatatgc aagtgcaagg
    17041 gaccgtgcct gtacagatgg cgcaattcgg atgcatgttt cagtgctgac atcaataaag
    17101 gtagccgcaa cctttccaaa atccatcaca cgaagcgtac ggttgccaac gcggcaccct
    17161 gtggcagcag aaattccatc caacgcacag ccgtctgttt ctgcgatcac caacagacgt
    17221 ttatcagcgc gtggaagctg caacccaaga agttcccccg catacaaccc catacgcaca
    17281 cccaatacct ggcgcggaca aagatggtcg tgatggcgcg aactttcgca caaaatctct
    17341 gcaagcatca tttttcttcc aacggagcaa atcccccacc gaacacttcc tgcgcaggcg
    17401 aaacaatgac caccgccttc gggtcttcct ttgccactgc agatttcaaa ttatgcactt
    17461 cggtaatggt caatgcacac atcaaaatgg aacgatcctt gcctgtgtac attcccttgc
    17521 ccagcatgga ggttactccg cgtttgagat cggtcataat tcgacctgat acagagtcgg
    17581 ccttatctgt aatgataaga gcaagcaact gtcgccttct tcggcgcggc acgagcaaag
    17641 cgcgcaaacc agtatgtttt ccctccatcg atggcgccgc cggtgcagac tcatcaccaa
    17701 aacgcggaag gatttcaccg gttgccttgg tcatattgat cgtggcaatc gcaaagatcg
    17761 cgagaaaggt ataaaaggaa atacccaaac cgatcataac ctcaccaggt acaggcccaa
    17821 cagacgaacg caccacaaac tcagacatgc ttgctggagc ttcagggaaa agatagatct
    17881 tcgccaccca tgaagccagc aggatcaaaa agatccagta ataatttctg cgcaaacgcc
    17941 tgccaaatgc ctcccacatg gagatgggaa aacttggcgt aagcaaattc tcggcaaggt
    18001 tctccgccca ctctggcgag ggatgaaacg gcggcacgag catggcggcg taaaaatccg
    18061 tttccattaa ccgcacacgg taactccata attcataata gcgatatcgt cttgcctcga
    18121 taaacaaaaa ccacaaaacc aaaagcgtat ttaacaaaac caccgagtga tgcacgttcg
    18181 actggctgaa ggcaatcgaa agcgtcgcac ccgtcacaac cactgcccaa ttcgtggtcg
    18241 tatccaggcg cgtacgccaa acattggcac gctgaatttc ggcgcggaag aaatgtacca
    18301 ttgccgtaac aaactcactg gttttgagtc gatacccacg atacgtccaa acaggctctt
    18361 caactgggat gggttcttcc tgcttcggct ttgatttcgc ttttgttgac ttttttactg
    18421 ccattggatt ttctttctgg atgaagaacc cgcttcc
//
LOCUS       DAOQJC010000078        18399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1408_length_18399_cov_10.054677, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000078 DAOQJC010000000
VERSION     DAOQJC010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18399
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1408_length_18399_cov_10.054677"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="PLE39_12290"
     CDS             <1..138
                     /locus_tag="PLE39_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNE69156.1"
                     /translation="KGYHPAHTFKDGCTGCAICALVCPDAAITVYREITKNAAPVTVT
                     A"
     gene            219..1331
                     /gene="vorB"
                     /locus_tag="PLE39_12295"
     CDS             219..1331
                     /gene="vorB"
                     /locus_tag="PLE39_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNE69157.1"
                     /translation="MPKELWKGNEAIAEAAVRAGLEAYFGYPITPQTEILEYLSRRMP
                     ELGRAFVQAESELGAINMVYGAACTGVRVMSSSSSPGVSLMMEGISYIAGTEVPAVLV
                     NVMRGGPGLGNIAPAQADYNQAVHGGGHGDYQPIVLAPASVQEAIDLMVLSFDLAEKY
                     RSVCMVILDGSVGQMMEPAQMPEMRPVQRANWDWATDGRMGKRERRVLSSIYIEPAEE
                     EKMNLRLLNRWRTIQANEVRYKEYFLDDAEIVIIGFGTAGRVALSAVREARAQGIKVG
                     LLRPVTVSPFPSAVIEQLAGKVDAFLVTEMNSGQMLEDVRLAVKGRVPVEFYGRLGGV
                     VPFPDEILNEIRRMAKSKMAVNANPRDAWLERMAAG"
     gene            1344..2111
                     /locus_tag="PLE39_12300"
     CDS             1344..2111
                     /locus_tag="PLE39_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNE69158.1"
                     /translation="MLAENVVCVRPEGFTESPTHYCPGCTHGVAHRLVAEVLQEMNVL
                     DKTIGVAPVGCSVFAYNYFDTDFVEAPHGRAPAMATGIKRVLPDRIVFTYQGDGDLAS
                     IGMGEIVHAAARGENLTVIFVNNANFGMTGGQMAPTTLPGMKTTSSQNGRDVETQGYP
                     IKMAEMLATLDGVGFCARRSLHDAKNIRLAKKAIRMAFETQARGLGFAMVELLSTCPT
                     NWGMTPVNSLKFVENSMVPAYPLGDYKISEAIKQIKI"
     gene            2183..2743
                     /locus_tag="PLE39_12305"
     CDS             2183..2743
                     /locus_tag="PLE39_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNE69159.1"
                     /translation="MQKEIIIAGFGGQGVLFGGQVLAYAAMDTGRDVTWIPSYGPEMR
                     GGTANCTVVIADHEIGSPLVKNPPLAIALNLPSFDKYEPMLASGGTLIVNQSMVDHSA
                     RRSDIHTIFVPCNEIAEEIGDKKLMNMVAIGAMLTALPEIKIEDVEKALEAHLPARHK
                     HLLPKNYEALKRGFEHAQKEWDKVPA"
     gene            complement(2832..3269)
                     /locus_tag="PLE39_12310"
     CDS             complement(2832..3269)
                     /locus_tag="PLE39_12310"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNE69160.1"
                     /translation="MEQKEVALSVSKAILNGEWDKLNGILSDDFTYTGDGMFLNKAQY
                     MDFMKGMRAAFSNMNFEFSHVISEGDAVSIRFVVTAKHTGSFMGGAATNKNLNFSGIF
                     IRKVKGDKVVQEWQATDLLGMMTQMGVGTLLGYAIFGVWLKKK"
     gene            complement(3322..3651)
                     /locus_tag="PLE39_12315"
     CDS             complement(3322..3651)
                     /locus_tag="PLE39_12315"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNE69161.1"
                     /translation="MNTWLTAIHIKPEHVEKFLAASTPFAGESLRESGLVSFALLQRV
                     DDIAQFSMFDVYHDAAARENHLTSAHYLAWLTIVTPLLAAPMTSLPFNPIFPPPEDWE
                     RHLETES"
     gene            3751..4134
                     /locus_tag="PLE39_12320"
     CDS             3751..4134
                     /locus_tag="PLE39_12320"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE69162.1"
                     /translation="MSTYPIDILNEYCPSRKVMELIGDKWTLLTLNAIARGINRHNAL
                     LREVGGISQKMLSQTLRALERNGIVTRHVFPVVPPRVEYELTLLGKSLNEPVVALGKW
                     AQEHYAQVEKAREKYDIEFKNDEEE"
     gene            4194..4592
                     /locus_tag="PLE39_12325"
     CDS             4194..4592
                     /locus_tag="PLE39_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNE69163.1"
                     /translation="MDKRINISSGAKWEDIVGYSRAVRVGNMIEVAGTTAVDERGEIV
                     GLNDPYEQTKYALAKIEKALIEAGASMQDVVRTRMFVTDISKWEEVGRAHGEFFRTVK
                     PAASMIEVKGLIDPKLLVEIEVTAIIAEAK"
     gene            4644..5816
                     /locus_tag="PLE39_12330"
     CDS             4644..5816
                     /locus_tag="PLE39_12330"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNE69164.1"
                     /translation="MEDISLVFIELGAAIIGLALLTRLASRLGLSAIPFYLLAGLAFG
                     NGGLLPIQFSEDFVHIGAEIGVILLLFMLGLEYSGEELGTNLRSGFPAGIVDFLLNFP
                     PGLILGFVLGWSPLAAVLLGGVTYISSSGVIAKVLSELGRMNNRETPTIISILVLEDL
                     IMAVYLPIVAVLLIGQEISQAVLSVSIALATVGTVLFVAVKYGKKFSKLIAHQSDEVI
                     LLTTFGLMLFVAGMAQYLQVSAAIGAFLMGIAISDSIAERTHKLLGPLRDLFAAIFFL
                     FFGLQIDPASLPPVLLLAVSLGLFTTLTKILTGWWAARRAKSNPLEAFRAGAALVARG
                     EFSIVIAGLGVSAGLEPQLGALSAAYVLFLAILGPILARFIEPIYRAFHKKQITSS"
     gene            complement(5803..7134)
                     /locus_tag="PLE39_12335"
     CDS             complement(5803..7134)
                     /locus_tag="PLE39_12335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE69165.1"
                     /translation="MHSSVHEIRTRLLSLLVRAFVFVLFLALIFFLAVVGAVLTSQTS
                     PIPIPFGRALEGYYIGHGNWDGVEKVFDTLEELKTLDVLLLDEGQRIVLDHDSDPASV
                     VGSSYEIRPNDFLINLTVEGQVVGRLAFTSITPWSRLGIARAVVIPVGIISLLLSIFL
                     LIVATVLVRRFVNPLADVIYAAREVTNGKLTTRISSEGPQDMRSLSESFNEMTAALER
                     NDRERRDLLADIAHELRTPLSVIRGRLEGIVDGIYTADDGQVSLALEQTYLLERLVDD
                     LRLLTLAETRQLHFEKKKVDFVQLSQHTIEMFSAEAREKNISISLEMGKGNYTAELDP
                     QRMEQVIGNLIGNSLRYIPEGSKIWLALEETSKEVKLFVNDNGPGVAQDELPYLFDRF
                     WRKDKSRTRSAGGTGLGLAIAKQLVEAQGGTIHASNLREGGLQIAIEFRKM"
     gene            complement(7136..7801)
                     /locus_tag="PLE39_12340"
     CDS             complement(7136..7801)
                     /locus_tag="PLE39_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE69166.1"
                     /translation="MPKKILIVDDEPQIVEICRDYLKAAGYETVTASNGSQALALARR
                     EKPDLIVLDLMMPEMDGLDVTREIRRASDVPIIMLTARVEETDKLIGLELGADDYITK
                     PFSPRELVARIRVVLRRVVNSPDGDTIRAGSVELDRAHYEVHLPDKTVSLTATEFEIL
                     ATLMSQPGRIFSRAQLLTATHGVTFESLERAIDSHVRNLRRKLEPADLIVTVHGVGYK
                     FEG"
     gene            complement(7912..9150)
                     /locus_tag="PLE39_12345"
     CDS             complement(7912..9150)
                     /locus_tag="PLE39_12345"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE69167.1"
                     /translation="MLYSENLKIALRALRANKMRSVLTILGIVIGVATVVALLSIGNG
                     ATASITDQIQSGGSNLLTLSPGRMQTPMSSLGQSQQASYLYYSDYETLSKTLTGEITS
                     IVPVYQASYTVKFGDESFSTSVSGVMEDHAQARSFTISAGRFVTDGDNDALARVAVLG
                     ATVAEDLFGTLSPIGQTISLNGVKFEVVGLLESEGASIGSPDDIIFVPLETGYIKLFG
                     DTANHNSKKTVNSILISVTDSKDMDAISAQAEYILRRAHELKPTEEIDFNIMSQADTL
                     ETLNSVTQTLTVFLGAIAAISLLVGGIGIMNIMLVSVTERTKEIGLRKAVGATKNQIL
                     TQFLIETVTLSLLGGLVGILLGVGIALAFSLSGLINSVITADSILLAFFFALAIGIFF
                     GLYPAQRAASLHPMVALRYE"
     gene            complement(9153..9869)
                     /locus_tag="PLE39_12350"
     CDS             complement(9153..9869)
                     /locus_tag="PLE39_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE69168.1"
                     /translation="MIQADGLTKVYQMGDSEVRALDGASFTIKKGEMVAIMGPSGSGK
                     STLMSIIGCLDVPSEGSYSLDGEAVQTLDETKLADVRGRKIGFVFQQFNLLARTSALE
                     NVMLPLTYAGISGKEREDRAKKALERVGLGGRTSHAPNELSGGQQQRVAIARAIVNEP
                     SILLADEPTGALDSKTGVEIMDLFQRLHRENGQTVILVTHDSYVARHTERIIKISDGR
                     IVSDEVNPNPIKAGTGRGEE"
     gene            complement(9866..11374)
                     /locus_tag="PLE39_12355"
     CDS             complement(9866..11374)
                     /locus_tag="PLE39_12355"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE69169.1"
                     /translation="MQNMLKNKRNWWIAAILVVAGVLVWAGVAATGSANAAEAQTEQV
                     IAMELAQTVETSGTLESQPFASLNWKTGGIVESVHVQPGDFVKAGDVLLTLQPESTSA
                     SLASAQADLETAQANLRALTFPDGSSVGEARENASSAFDAWSDARVDLMDALAYNRGG
                     GDDDLYSDVVTARDDLVNALEEYPLAANVDAQFYYWAARADALGYHGEYDHAALVPVF
                     RAELNADDAELVDEILTAQTEFETLAKAFAESMEDQDDAVDAMKAFGAYEQSADALLD
                     AMEAEYGVLVQPSESDLASAQAKVDAAQASVNNLQIVAPFDGQVLSVDDLVGDTVSAG
                     DLSVNMADMNNLYVEAQVDESDVANVRVGNQVEVTLDALPDLVLTGSVTVVNPVGEVV
                     GGLVKYSIRVELDPVKDDFLPLGATANVVIKVSDTQASLAVPIMAIQNDNEGEYVWVL
                     RDGEAVRVNVTGGALVDGYVVVTGDLQEGESLQLVERESGFTPPNPFSGGAK"
     gene            11485..12372
                     /locus_tag="PLE39_12360"
     CDS             11485..12372
                     /locus_tag="PLE39_12360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNE69170.1"
                     /translation="MTNKITINIINAFTDAGEGGNPAGVVYPADEFTKEDKQKIAAKV
                     GLSETAFVSRSQSAEFKLDFFTPNKQIAHCGHATIATFSYLSQQGIVKGPKSSKETID
                     GNRDIYLKGEVAYMQQLAPKYFPLPYKGISEQDVLASLHLQKTDLLAGHNPMPVNTGN
                     SFMMIPVKDEATLKAIHPDLNAIHQISEALDLIGYYPFTLETQVRGRDAATRMFAPYY
                     GIPEEAATGMAAGPLACYLFDQININKETLILEQGRLMPTPSPSEILVELKLEDGSIK
                     SLLAGGRAKVTRSIEIELD"
     gene            12380..13201
                     /locus_tag="PLE39_12365"
     CDS             12380..13201
                     /locus_tag="PLE39_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE69171.1"
                     /translation="MKFETLPNLTLPKIGFGTWKIGGESTPDPSLDSVSMAALRSALE
                     VGYTHFDTAEMYSSGHAEELIGRVVRETKTKREDLFITTKVSPEHLNPKDVFSSCEKS
                     LRRLQMEYVDLYLIHWPSTGMKLEETFTALNKLVRDGKVRHLGVSNFKLRDLKKSQEL
                     SETPLLTNQVPYRLPDRAYVENGVVEYCQKNDILITAYSPVKFRNLPVNKTLQEIAEA
                     HSATAYQIALAWLVSQPRVITIPMSFNPQHILENFEAAEIVLTSQEVEQLNRLYI"
     gene            13218..13523
                     /locus_tag="PLE39_12370"
     CDS             13218..13523
                     /locus_tag="PLE39_12370"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNE69172.1"
                     /translation="MNNQIAPEIEIIEITETELEPLYRILIHNDDVTPMDFVVHALTT
                     FFYLGTPTAAEIMLKAHITGMAYVQTVAKSEAERRINKAHFAAGLEGYPLHFSMEPE"
     gene            13819..14415
                     /locus_tag="PLE39_12375"
     CDS             13819..14415
                     /locus_tag="PLE39_12375"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE69173.1"
                     /translation="MAGMTRGKGLLEPMLADLRAQRANKLIPSNLRNGRILDIGCGSF
                     PYFLAHTAFAEKFAIDQIPLPQQTASELKIESFTLDLEIEPRLPFENDYFESVTLLAV
                     VEHLDPALMAKLFKEIYRVLKPEGMVILTTPAAWSDGLLKFMARIGLVSAEEIHEHAY
                     AYTLPLLGWYFGQAGFEMTKTKFGYFEFMLNMWATAKK"
     gene            14417..15271
                     /locus_tag="PLE39_12380"
     CDS             14417..15271
                     /locus_tag="PLE39_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69174.1"
                     /translation="MDQFETLTQINLDDLVSSFGWQNHPLAAKILRFLFRKPAETFAR
                     HMMEYDDTTGMHGIAEGGRNLLNRYVKELRVLHADRVPDSAFLALSNHPGMTDTVALF
                     AALNRKDLHIIALDRPFLNALPHTTRQLFYVHDDPAKRMTLVRQVSSHLKNGGAALTF
                     PAGHIEPDPEIYPGAVDSLQAWTDSVGVFVRMAPEAAILPVLVRGVVSAKYADHWLLK
                     IKKTQEEKEKLATALQLLGMVMFNEKPVTVTVQIGNPIYVKALGTNDAAAIHQAVLAE
                     MKNLIESS"
     gene            complement(15302..16588)
                     /locus_tag="PLE39_12385"
     CDS             complement(15302..16588)
                     /locus_tag="PLE39_12385"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE69175.1"
                     /translation="MHIPPSLKHKKFFYLWLGQLISITGTQMQLWALFWHVSNLDKNP
                     IALGGIGLARILPVIVFSLIGGALADSVDRRKVMFVTQSAAALLALTLGLLTQFGQVT
                     IWYIYVITAIQAIAIAFDGPSRQALIPNLVPKEDLPNAFSMTFTSFQFGSVLGPALSG
                     FVIAWAGQEAVYYFNAISFGAVLFALFMIGDVPQSFAEKSAGISMDAIREGIRFILGK
                     PIILSSMLLDFVATFFASANTLMPIIATTVLKVGVVEYGYLSAASAVGAVTVALAISQ
                     VKVIRRQGPMFMGAVIVFGIATVIFGLTRSFWVAWLAIATTGAADGVSTIIRNTIRQL
                     QTPDHIRGRMTSINQIFFQGGPQLGEMEAGSVAQFFGASFAVISGGIGCIIGTLLIMW
                     KWPQLMHYDGHEPIEGEIAPSPSSGASIVSNRVRRP"
     gene            complement(16662..17489)
                     /locus_tag="PLE39_12390"
     CDS             complement(16662..17489)
                     /locus_tag="PLE39_12390"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNE69176.1"
                     /translation="MTDLNLTINADLAREILTGFIKSEITRVGLTRAVVGLSGGVDSA
                     LSCALAAEALGPQNVLAVRMPYKASSKDSLEHAQLLIDQLGVQSKTIEITDMVEPLFK
                     LHPDISNMRKGNIMARERMIVLYDQSEVFKGLVVGTSNKTEILLGYSTLFGDSANALN
                     PIGDLFKTQVRQLSRAMNIPAPILEKPPSADLWADQTDEKELGFTYEEADKLLYLLVD
                     QRYSPQEAIEVGFAEDFVKTVTTRIRRNQFKRMQPPIAKVSNRTIGYDFLYLRDWGT"
     gene            complement(17482..>18399)
                     /locus_tag="PLE39_12395"
     CDS             complement(17482..>18399)
                     /locus_tag="PLE39_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNE69177.1"
                     /translation="FFSILSISWGILFSDKRGKIRPMKLNLALAQINTRLGDVEANLA
                     KHLEYIEQAKAEKTDLLVFPELSLTGYVLQDLVASAAHKPTEDDPIFKHLLKASHDLD
                     LVIGFVDEDSRHRFYIASAYLSGGKAIHVHHKVYLPTYGLFDEGRFFAWGDSVRAFNT
                     RFGRMGMLICEDFWHASPPYLLWLDGADIMLFSSASPGRGLNDKEKLESARWVERVTK
                     AYASMFTSFVAHANRVGYEDGLHFWGGATAFDPNGEELAHGPYNEEAITYTQLDLNQL
                     RRTRARLPLLRDERTALIRNELDRILSRD"
BASE COUNT         4565 a         4919 c         4680 g         4235 t
ORIGIN      
        1 aagggctacc accccgcgca cacgttcaag gacggttgca cggggtgtgc tatttgtgcg
       61 ctggtctgcc cggatgcagc gatcaccgtg tatcgtgaaa taacgaagaa cgccgccccc
      121 gttacagtaa ctgcataaaa agatgaaaga agaaggaaat ttacatcttt cttctttcat
      181 ctttctcttt tgaactacaa aaaccaatgg agaaataaat gccaaaagaa ttatggaaag
      241 ggaacgaagc cattgccgaa gctgccgtac gcgcggggct ggaggcgtat tttggttacc
      301 cgatcacccc acaaacagaa atccttgaat acctctcccg ccgcatgcct gagctgggac
      361 gtgcttttgt gcaggcagaa tcggaacttg gcgcgatcaa tatggtctat ggcgcggcct
      421 gcaccggggt gcgtgtgatg tcatcttctt caagtccggg ggtgagcttg atgatggaag
      481 gcatctccta catcgcaggg acggaagtgc ccgcggtgct tgtcaacgtg atgcgcggcg
      541 gaccaggcct cgggaacatt gcccctgctc aggccgatta caaccaggct gtccatggcg
      601 gcgggcatgg ggattatcaa ccgatcgtgc tggctcctgc ttcagtgcag gaagccattg
      661 acctgatggt gctttccttt gatctggcag aaaaataccg ctctgtgtgc atggttattc
      721 ttgatggttc ggtgggacaa atgatggagc ctgcccaaat gcctgagatg cgacccgtgc
      781 agcgggccaa ttgggattgg gctaccgatg gcaggatggg caaacgcgaa aggcgtgtgc
      841 tttcatccat ttatattgag cctgccgaag aagaaaagat gaatctgcgc ctgctcaatc
      901 gctggcggac cattcaagca aatgaagtac gctacaagga atatttcctt gatgatgccg
      961 aaattgtgat tatcggcttt ggcacagcag gacgtgtggc gctttcagcg gtccgtgagg
     1021 cccgcgcaca gggaatcaag gtggggttgt tgcgcccggt cactgtcagc ccgtttcctt
     1081 ctgcggtgat cgagcagctt gcgggtaaag tggatgcgtt ccttgtcact gagatgaatt
     1141 ccggtcaaat gctggaggat gtccgcctgg ctgtaaaagg ccgcgtgccg gtcgaattct
     1201 atggaagatt gggcggcgtg gttcccttcc ccgatgaaat tctgaacgag attcgccgca
     1261 tggcaaaaag caagatggcc gtgaatgcca atccgcgtga tgcgtggctt gaacgcatgg
     1321 cagcagggta aaggagagac agaatgctgg ctgaaaatgt tgtttgtgta agacctgaag
     1381 ggtttaccga atcacccacc cattattgcc cgggttgcac gcatggggtg gcacatcggc
     1441 ttgttgcaga agtgctgcag gaaatgaatg ttttggataa aacgatcggt gttgcgccgg
     1501 tgggctgttc ggtgtttgcc tacaattatt tcgataccga ttttgtagaa gcgccgcacg
     1561 ggcgtgcgcc cgcgatggca acgggcatca agcgtgtgct gcctgaccga attgttttta
     1621 cctatcaagg cgatggcgat ctggcttcga tcggcatggg tgaaattgtc catgcggcgg
     1681 cacggggcga gaacctgacc gtgatctttg taaataatgc caacttcggc atgaccggtg
     1741 gacaaatggc gccgaccaca ctgcctggca tgaagaccac ttcttcacag aatggccgcg
     1801 atgttgaaac gcagggatac ccgatcaaaa tggctgaaat gctcgccaca ctggatgggg
     1861 tcggattctg tgcacgccgt tcattacacg atgcgaagaa catccgcctg gcgaaaaagg
     1921 cgatccgcat ggcgtttgag acccaggccc gtggacttgg ttttgcgatg gtcgaattgc
     1981 tttccacctg cccgacgaat tggggcatga ctcccgtcaa ctcgctgaag ttcgtggaaa
     2041 acagcatggt ccccgcctat ccacttggtg attacaagat cagtgaagcg atcaaacaga
     2101 taaaaatcta gtgagtagtg attagtgaat agtgatcggc actccattat ctattcacta
     2161 ttctctgctc caaaggagca acatgcaaaa agaaatcatc atcgcaggct ttggcggaca
     2221 gggtgttttg ttcggcggtc aggtgctggc gtatgcggcc atggatacag gcagggatgt
     2281 gacttggatc ccgtcctatg gacctgaaat gcgcggcggc acggccaatt gcaccgtggt
     2341 catcgcggac catgaaattg gttcgccatt ggtgaagaat ccacctttgg caattgccct
     2401 caacctgccg tcttttgata aatatgagcc catgctggcc agcggcggaa ccttgatcgt
     2461 gaaccagtcc atggtggatc attcggcgcg gcgcagcgat atccacacga tttttgttcc
     2521 ctgcaatgaa atcgctgaag aaatcggcga caagaaattg atgaacatgg tggccatcgg
     2581 agcaatgctc accgccctgc ctgagatcaa gatcgaagac gtggaaaaag cgctggaggc
     2641 tcatttacct gcaaggcaca agcacttgct gccaaagaac tatgaagccc tgaagagagg
     2701 ctttgagcac gcacagaagg aatgggataa ggttccagcg tgaagcagta attggtaatt
     2761 ggtaattggt aattggtaaa gcgtccattt cgtgttgaaa tggacgcttt tgtttgggga
     2821 caattatctt gttatttctt tttgagccac acaccaaaga tggcgtagcc gagcaaggta
     2881 ccaacaccca tttgagtcat catgccgagc aggtcggtgg cttgccattc ctgcaccact
     2941 ttatcaccct tcaccttccg tatgaaaatt ccgctgaaat tgaggttttt gttcgtcgcc
     3001 gccccaccca tgaaactgcc tgtgtgcttt gcggtcacaa caaagcggat gcttactgca
     3061 tcgccctcgc tgatcacatg tgagaattca aagttcatgt tgctaaatgc agctctcata
     3121 cctttcatga aatccatgta ctgcgctttg ttgaggaaca ttccatcgcc cgtataggta
     3181 aaatcatcgg acaaaatccc attcaactta tcccattctc cgttgaggat ggctttcgaa
     3241 acactgagtg caacttcttt ttgttccatt ttatttactc ctgtttttaa tttgattttt
     3301 tacgaaaacc ctaatcgaag atcacgattc cgtttccaga tggcgttccc aatcttcagg
     3361 cggcggaaag atcgggttga atggcaggga ggtcattggt gccgccaaaa gcggtgtgac
     3421 aatggtcaac catgccagat agtgcgccga ggtcaggtga ttctcgcggg cagcagcatc
     3481 atgatagaca tcgaacatcg aaaactgtgc aatatcatcc actcgttgta aaagcgcaaa
     3541 gctgaccaat ccgctctcgc gcagactctc tcccgcaaaa ggagtcgatg ccgccagaaa
     3601 tttttcaacg tgctctggtt tgatgtgaat tgcagtaagc catgtgttca ttgtcatctt
     3661 ttcctgttct gttacaatca gtataagtgg cgggtcaaac ccttccaaga gcccactaat
     3721 ttgtatggta gttaccaaaa ggtgtgtatc atgagcacat acccaataga tatcctcaac
     3781 gaatattgcc cttcgcgcaa agtgatggaa ttgatcggag acaaatggac tctgctcaca
     3841 ttgaacgcca ttgcacgcgg aatcaaccgt cacaacgcct tattgcgtga agttgggggc
     3901 atctcacaga aaatgctttc tcaaaccctg cgagcacttg aacgcaatgg catcgtcaca
     3961 cgccatgtat ttcctgtcgt accaccccgc gtggaatacg aattgaccct gcttggcaaa
     4021 tcgctcaatg agcctgtggt ggcacttgga aaatgggcac aggaacatta cgcacaggtg
     4081 gaaaaagcac gcgagaaata cgacatcgaa ttcaaaaacg atgaagagga ataggcaaag
     4141 atcccgcctg aaaagcgggg ttttttattt tgtatcaaaa tggagttgac accatggaca
     4201 aaagaatcaa catctcttca ggtgcaaagt gggaggatat tgttggctat tcgcgagcgg
     4261 tcagggttgg caatatgatc gaagtggcag ggacaactgc ggtcgacgag cgaggcgaaa
     4321 tcgtcggttt gaatgatcct tacgaacaga ccaaatacgc cctcgcgaag atcgaaaaag
     4381 ctttaattga agctggcgca tccatgcaag atgtggtgcg aacccgcatg ttcgtgaccg
     4441 acatttcaaa atgggaagag gtcggcaggg cacatggtga atttttcaga acggtcaaac
     4501 ctgccgcatc catgatcgaa gtgaaagggt tgatcgatcc taaattattg gttgaaatag
     4561 aagtcactgc gatcattgca gaagctaaat aagaaaattc acatggaatt gaacacgggg
     4621 agctttcttg tgtaaaatca agtatggaag acatctccct tgtttttatt gagcttggag
     4681 cagccatcat tggccttgcc ctgctcacac gccttgccag ccgtttaggg ctttcagcca
     4741 tcccctttta tttactggca ggcctcgcct tcggcaatgg cggcctcctc cccattcaat
     4801 tcagcgaaga ctttgttcat atcggcgcag agatcggtgt cattcttctg cttttcatgc
     4861 tggggcttga gtattcaggc gaagagctcg gcacgaatct gcgttcagga ttcccagctg
     4921 ggatcgtgga ttttctgctg aacttcccgc ctggtctcat tcttggtttc gtgcttgggt
     4981 ggtcaccact tgccgcagta ttgcttggcg gcgtcaccta catctcatcc tctggcgtca
     5041 tcgcaaaagt attatccgag ttggggcgta tgaacaatcg cgaaacgccc accatcattt
     5101 ccatcctcgt gctcgaagac ctcatcatgg cggtctatct gcccattgtg gctgtgctgc
     5161 tgatcgggca ggagatctcg caagcggttc tctccgtttc cattgcactt gcaaccgtcg
     5221 gcacagtttt attcgtcgct gtcaaatacg ggaaaaaatt cagcaagctc atcgcgcatc
     5281 aatcagacga agtcatcctg ctcaccacat tcgggttaat gctcttcgtg gcaggcatgg
     5341 cgcaatacct gcaagtctct gcggccatcg gtgcattttt gatgggcatc gccatctccg
     5401 actccatcgc cgagcggact cacaaactgc ttggcccatt acgcgactta tttgccgcca
     5461 tcttcttcct tttctttgga cttcagatcg accccgcttc tttacctccc gttttgctgc
     5521 tggctgtttc tctgggcctt ttcaccacgc tgacaaagat cctgacgggc tggtgggcag
     5581 cccgccgtgc caaatcaaat ccgttggaag cgtttcgtgc aggtgcagca ttggtggcac
     5641 gcggagaatt ttcaatcgtc attgcagggc tgggagtcag tgcaggcttg gagcctcaac
     5701 tcggcgcgct ttccgctgct tatgttttgt tccttgccat tcttggcccg atccttgcaa
     5761 ggtttatcga accgatctat cgggcattcc acaaaaagca gatcacatct tcctaaattc
     5821 gatcgcaatt tgcaaaccac cctcacgcaa attgctggcg tggatcgtcc caccctgcgc
     5881 ttcgacaagc tgcttcgcaa tggcaaggcc caaacccgtg ccgcccgcac tgcgtgtgcg
     5941 ggatttgtct ttgcgccaga aacgatcgaa aaggtagggc aattcatctt gagccacacc
     6001 aggcccgtta tcattgacga agagcttcac ctcttttgat gtttcctcaa gcgcaagcca
     6061 tattttgctc ccctctggga tatagcgcag tgagttccca atcaaattac caataacctg
     6121 ttccatgcgc tgcggatcaa gttctgctgt gtaattcccc ttccccatct caagagaaat
     6181 cgaaatattt ttctcccgcg cttcggcaga gaacatttca atggtgtgtt gtgagagttg
     6241 aacgaaatca actttctttt tctcgaaatg caattgacgg gtctcggcaa gggtcaacag
     6301 acgcaggtca tccacaaggc gttcgaggag ataggtttgc tcaagggcga gggatacttg
     6361 tccatcatcg gcagtgtaga ttccatccac aatgccttcg aggcggccgc gaatgacaga
     6421 gagcggtgtg cgcagttcgt gggcaatatc tgcaagcagg tcacggcgtt cacggtcgtt
     6481 gcgttcaagg gcggcggtca tttcattgaa gctttcagac aggctgcgca tgtcctgtgg
     6541 accttcactg gagatgcggg tggtgagttt cccgttggtc acctcacggg cagcatagat
     6601 gacatctgcc agcggattga cgaaacgacg aaccaatacc gtggcaacga tcaaaagaaa
     6661 aatggaaagc aacagtgaaa taatgccgac aggaatgacc accgcacgcg caatgcccag
     6721 cctgctccag ggggtgatcg aagtgaaggc aagccgcccc accacctgtc cctccaccgt
     6781 cagattgatc aggaaatcgt tgggtcttat ttcgtagctg gaacccacca ccgatgcagg
     6841 atcagaatca tggtccagaa cgatgcgttg cccttcatcc agtaaaagca catcaagggt
     6901 cttcaattct tcgagggtgt caaaaacctt ttcgacccca tcccaattgc cgtggccgat
     6961 gtaataccct tccaatgccc gcccgaacgg aatgggaatc ggcgaagttt gcgaggtcaa
     7021 aacagccccc actactgcaa gaaagaaaat caacgccaga aatagaacga atacaaaagc
     7081 cctgaccagc agggacaaca aacgggtgcg aatctcgtgg acagatgaat gcatcttagc
     7141 cctcaaattt atacccaacg ccatgcacgg tgacgatcaa gtcagcgggc tccagtttac
     7201 ggcgcaggtt gcggacatgt gaatcgatgg cacgttcgag gctttcaaaa gtgacgccat
     7261 gagtggcggt gagcagctgc gcacgagaaa aaatacggcc aggctgactc atgagcgtgg
     7321 cgaggatttc aaattcagtg gcagtgagcg aaaccgtttt atctggcagg tgaacttcat
     7381 agtgcgcacg atccagctcc acactgcctg cgcggatggt atcgccatcg ggagaattta
     7441 caacgcggcg caaaacaaca cggatgcgcg ccaccaattc acgcgggctg aacggtttgg
     7501 tgatgtaatc atctgcgccg agttcaaggc cgatgagttt gtcggtttcc tcaacgcggg
     7561 cggtaagcat gatgatgggc acatcgcttg cacggcgtat ttcgcgggtg acgtccagac
     7621 cgtccatttc tggcatcatc aaatcaagca cgatcaaatc tggcttttca cggcgtgcca
     7681 gtgcaagcgc ctgtgaaccg ttgctggctg tgacggtttc ataacctgcc gctttgaggt
     7741 aatcgcggca gatttccacg atctggggtt catcatccac gatgaggatt ttttttggca
     7801 tgggacgatt atacgagact atgtgaagag ggtgtgtttt ttgggagtgg aaagtggaaa
     7861 gtataccgac atcctacttt ccactttcca gacattacca atgatttgag tttattcata
     7921 cctcaatgcc accatcgggt gcagggatgc ggcccgctgg gctgggtaga ggccaaaaaa
     7981 gatgccaatg gcaagcgcga agaaaaaggc cagcaaaatc gaatcggcgg tgatgacgga
     8041 gttgatcagg ccagagaggc taaacgccag agcaatgccc accccgagca aaatccccac
     8101 caaacctccc aacaaactca acgtgaccgt ttcgatgagg aactgggtca gaatctgatt
     8161 cttcgttgca ccgactgcct tccgcaggcc aatctccttg gtgcgttcgg tcactgaaac
     8221 cagcatgatg ttcatgatgc caatgccgcc caccagcagg gaaattgcgg caatcgctcc
     8281 gaggaagacg gtcagggttt gggttacaga attcaaggtt tcgagcgtat cagcctgact
     8341 catgatattg aaatcgatct cttccgtggg ttttaattca tgggcgcggc gcagaatgta
     8401 ttccgcctgg gcagagatgg catccatgtc ttttgaatcg gtgacagaga tcaaaatgct
     8461 gttcacggtc ttcttgctgt tgtggtttgc cgtatcaccg aaaagtttaa tgtaacccgt
     8521 ttccagcggc acaaagatga tgtcatcggg ggagccaatg gaggctcctt ctgactcaag
     8581 cagccccacc acttcaaact tgaccccgtt caaagagatc gtctgcccaa tcggcgaaag
     8641 tgtgccgaat aggtcttccg ccacagtagc ccccagcaca gccacccgcg ccagtgcatc
     8701 gttatctcca tcggttacga agcgcccagc actgatggtg aacgaacgtg cctgcgcatg
     8761 gtcttccatc acacctgaaa ccgaggttga aaaactttcg tcgccgaatt tcacggtgta
     8821 gctcgcctga taaacaggca cgatcgaggt gatctcaccc gttaatgttt ttgagagcgt
     8881 ttcataatct gaataataca agtagctcgc ctgttgtgac tgccccaaac tggacatggg
     8941 agtttgcatc ctgcctggag aaagggtcag caggttggag ccgccgcttt gaatctgatc
     9001 ggtgatgctg gcggttgcgc cgttgccgat cgaaagcagt gccaccaccg tggccacacc
     9061 gatgacaatg cccaaaatgg tcagcacaga gcgcattttg ttcgcgcgca gggcacgcag
     9121 ggcgatcttg agattttctg aatacaacat ggttactcct ctcccctgcc cgtacccgcc
     9181 ttgatgggat tcgggttcac ttcatctgaa acgatccttc catcggagat tttgatgatc
     9241 cgctcggtat gacgggctac gtacgagtca tgtgtcacca aaatcaccgt ctgaccattt
     9301 tccctgtgca ggcgttggaa caagtccatg atctccacgc cagttttact atccaacgcg
     9361 cctgtcggtt catccgcaag caaaatactc ggctcgttga caatggctcg tgcaattgcc
     9421 acacgctgct gttgcccgcc tgagagctcg ttcggcgcgt gactggtccg cccgccaagc
     9481 ccaacacgtt ccagcgcctt tttagcacgg tcttcgcgtt ctttgccaga aatgcccgca
     9541 taggtcagtg gcagcatcac attttccagg gcgctcgtcc gtgccagcag attaaactgt
     9601 tgaaacacaa acccgatctt ccgtccgcgc acatcggcaa gctttgtttc atccaatgtc
     9661 tgcacggctt caccatcgag cgagtaactc ccttcgctgg gcacatccag acagccaatg
     9721 atcgacatca acgtgctttt gcccgaccca gaaggcccca tgatcgccac catctcgcct
     9781 tttttgatgg tgaaactcgc cccatcaagg gcgcgcactt cactgtcacc catttggtaa
     9841 accttggtca aaccatcggc ttgaatcatt tcgcaccacc agaaaatgga ttgggcggag
     9901 taaagccgct ttcacgttcc acaagctgca aagactcacc ttcctgcagg tcaccagtta
     9961 ccaccacata cccatctacc agagcgccgc ctgtcacatt cacacgcacc gcttcaccat
    10021 ctcgcagcac ccacacatac tcgccttcat tgtcattttg aattgccatg atcggcactg
    10081 ccagagaagc ctgtgtatca ctgaccttga taaccacatt tgccgttgcg cccaggggga
    10141 ggaaatcatc tttcacgggg tcaagctcca cacggatgga atacttcacc aaaccgccca
    10201 cgacttcacc cacaggattg accaccgtca cagaacctgt caacacgagg tcaggcagcg
    10261 catccaacgt gacctcaacc tgattcccaa ccctgacatt ggccacatcc gattcatcca
    10321 cctgggcctc cacgtacagg ttattcatat cagccatgtt caccgaaaga tcgccagcac
    10381 tcacggtatc gcccaccagg tcatccactg aaagcacctg cccatcgaag ggagcaacga
    10441 tctgcaaatt atttacgctg gcctgagcgg cgtccacttt tgcctgagct gaagccaggt
    10501 cactttcgct gggctgaacc agcacaccgt attccgcttc catagcatcc aacaaggcat
    10561 cagccgattg ttcgtaggcg ccaaaggctt tcatcgcgtc gaccgcatca tcctgatctt
    10621 ccattgattc ggcgaaggct ttggccaaag tctcaaattc ggtctgggct gtgaggatct
    10681 catcgacaag ttcagcatcg tcggcgttca actcagcgcg gaaaacaggt acaagggcgg
    10741 catgatcata ctcaccgtga taccccaatg catcggcgcg ggccgcccaa taataaaatt
    10801 gtgcatccac attcgccgcc agcggatatt cctccagcgc attgacaagg tcgtcgcggg
    10861 cagtcaccac atcgctgtac aaatcgtcat cgccgccgcc gcgattgtag gcaagtgcgt
    10921 ccatcagatc cacccgtgca tcgctccacg cgtcgaacgc gctggatgca ttctcacggg
    10981 cttccccaac ggatgaacca tcagggaagg tcaatgcccg caggttggct tgagcagttt
    11041 ccaggtccgc ttgagccgag gccagagaag cggatgtgct ctcaggctgc aaagtcaaca
    11101 ggacatcgcc tgcttttaca aaatccccag gctgcacatg gaccgattca acaattccgc
    11161 ctgtcttcca attgaggctg gcaaagggct gggattcaag tgtgcctgaa gtttcaacag
    11221 tttgtgccag ttccatcgca atgacctgtt ctgtttgtgc ttctgccgca tttgcagaac
    11281 ctgtggcggc cacacccgcc caaaccaaca caccagcaac cacgaggatg gcggcaatcc
    11341 accaatttct tttgttcttt agcatatttt gcatgattgt tctccttcaa attttccaaa
    11401 ggatactttt tagatgtgca gaatttgtgc agaatatcgg gagagaaatg gatgctacaa
    11461 tagccataca attacagagg acccatgacc aacaagatca ccatcaacat tatcaatgca
    11521 ttcacagacg cgggagaggg cggcaatccc gctggcgttg tttaccctgc cgatgaattt
    11581 acgaaagagg acaaacaaaa gatcgccgca aaggtcgggc tttctgaaac ggcatttgtt
    11641 tccagatcac aaagcgcgga attcaagctg gatttcttca cgcccaacaa acagatcgct
    11701 cactgcggac acgccaccat cgccacgttc tcctatttgt cgcagcaggg aattgtgaaa
    11761 ggccccaaaa gctcaaaaga aaccatcgat ggaaatcgcg atatttattt aaagggtgaa
    11821 gtggcgtaca tgcaacaact tgcgccaaag tattttccgc tcccctacaa aggcatcagc
    11881 gaacaggatg ttctcgcctc actgcacctg caaaagaccg acctgctggc tggtcataac
    11941 cccatgcccg tcaacacagg caattcgttc atgatgattc ccgtgaagga tgaagccacg
    12001 ctcaaagcga tccaccccga cctgaacgcc attcaccaga tcagcgaagc gttggattta
    12061 atcggctatt atcccttcac gctcgaaaca caagtccgcg ggcgcgatgc cgcaacacgc
    12121 atgtttgcac cgtattacgg cattcccgaa gaagcggcca caggcatggc ggcgggaccg
    12181 ctggcatgtt atttattcga ccaaataaac atcaacaaag aaacgctgat tctcgaacaa
    12241 ggcaggctca tgccaacgcc atcacccagc gaaattctgg tcgaattgaa actggaagat
    12301 ggcagcatca aaagcctgct cgctggcggc agggccaaag tgacacgctc gatcgaaatt
    12361 gaattggatt aatacaccga tgaaatttga aaccctccca aacctgaccc tccccaagat
    12421 cggattcggc acctggaaga tcggcggcga atcaaccccc gacccgagtc ttgattctgt
    12481 ttcgatggcc gctctgcgct ctgcgctgga ggttggctat acccacttcg acaccgcgga
    12541 aatgtattcg agcgggcacg ctgaagaatt gatcgggcgt gtggtgcgcg agacaaagac
    12601 caaacgtgaa gacctgttca tcaccaccaa ggtttcaccc gaacacctca accccaagga
    12661 tgttttttca tcctgtgaaa aaagcctgcg ccgcctgcaa atggaatatg tcgatctgta
    12721 tctcatccac tggccttcga cgggcatgaa actggaagag accttcaccg cgctgaacaa
    12781 acttgtccgc gacgggaaag tgcgccattt gggagtgagc aacttcaaac tcagagacct
    12841 caagaagtcg caggaacttt ctgaaacacc cctgcttacc aatcaggtac cctaccgcct
    12901 gccagaccgt gcctacgtgg aaaatggcgt ggtggaatat tgccagaaaa acgatattct
    12961 gatcacggca tattcacccg tgaaattccg caacctgcct gtgaacaaaa ccctgcagga
    13021 gatcgccgaa gcacattccg ccacagccta tcagatcgcg ctggcatggc tggtctccca
    13081 accgcgcgtc atcacgattc ccatgtcgtt caacccgcag cacatcctgg aaaatttcga
    13141 agcagccgag atcgtcctca cctcacaaga ggtggaacaa ctaaaccgcc tttatatata
    13201 aaaaattcat ccttttcatg aacaaccaaa ttgcccccga aattgagatc atcgagatca
    13261 ccgaaacgga actcgagccc ctctaccgca tcctcatcca caacgatgat gtgacgccga
    13321 tggatttcgt cgtgcatgcg ttgacgacct tcttttatct cggcacaccc accgccgccg
    13381 agatcatgct caaggcccac atcacaggca tggcctacgt gcagaccgtg gccaaatctg
    13441 aggcggagcg gcgcatcaac aaagcccact tcgccgcagg gttggaggga tatccgctcc
    13501 atttttcgat ggaaccagaa taacgggctt tcattgcagc gaatcgctgg gtccccgcct
    13561 ctcaacaaat ccgtagattg catggaaaat aaatgtggga tatagttgca cgaatttcat
    13621 ggtatcgcat aatctaccag gcaaagatca ggtagtctgc cgaaataaat ttcataaaaa
    13681 catgctctcg gcggcgtccc cgccgccgag gacggcggct tgagcgagaa aagttttgta
    13741 aactctttca gacgactaat tatgccccta atgtaattta cgcaaaaagc gataattcaa
    13801 aaattggaga acattttcat ggcaggtatg acccgcggaa aaggtctgct cgaacccatg
    13861 ctcgcagacc tgcgcgcaca acgagcgaac aagctcatcc cctcaaatct acgcaacggc
    13921 cgtatcctcg atatcggttg cggctctttt ccatattttt tggcacacac ggcctttgct
    13981 gaaaaatttg cgatcgacca gatcccgctc ccccaacaaa cagcctccga actgaagatc
    14041 gaaagtttca cccttgatct cgagatcgaa ccgcgcctgc catttgaaaa tgattatttc
    14101 gaatcggtca ctttgctggc cgtcgttgaa cacctcgacc ccgccttgat ggcaaaactt
    14161 ttcaaagaga tctaccgcgt gctaaaaccc gaaggcatgg tcatcctcac cacccccgca
    14221 gcatggtccg acggactgct gaaattcatg gcgcggatcg gccttgtcag cgccgaagag
    14281 atccatgaac atgcctacgc ctacaccctg ccgctgctgg gctggtattt cggccaggct
    14341 ggttttgaaa tgaccaaaac aaaattcggc tattttgaat tcatgctcaa catgtgggcc
    14401 accgcaaaga aataaattgg accagttcga aacactcaca cagatcaacc tcgacgacct
    14461 cgtctcctct tttggctggc aaaatcatcc ccttgccgcc aaaattttgc gattcctttt
    14521 ccgcaagcct gccgaaacct ttgcccgtca catgatggaa tatgacgaca ccaccggcat
    14581 gcacggcatt gccgaaggcg gacgcaacct gttgaatcgc tatgtaaaag aattgcgcgt
    14641 ccttcacgcg gaccgtgtgc ctgattcagc gtttctggca ttatccaatc atccaggcat
    14701 gaccgacacg gtcgcactct ttgccgcctt aaatcgcaaa gacctgcata tcatcgccct
    14761 tgatcgccct tttctgaatg cgctccccca cacaaccaga cagctattct acgtccacga
    14821 tgaccccgcc aagcgtatga cccttgtgcg tcaggtcagt tcccatttga agaacggcgg
    14881 cgcggcgctc accttccctg caggtcacat cgaacccgac cccgagatat acccaggcgc
    14941 agttgactcc ctgcaagcat ggacagatag tgtcggcgtt ttcgtgcgca tggctcctga
    15001 agcggcgatc ctgcccgtgc tggttcgcgg cgtggtctct gcaaaatacg ccgatcactg
    15061 gctgttgaaa atcaaaaaga cacaagaaga aaaagaaaaa cttgccaccg ccctgcaatt
    15121 gctgggcatg gtcatgttca atgaaaagcc agtcacagtg actgtgcaga tcggaaatcc
    15181 gatttatgtc aaagcattgg ggacaaatga tgctgcagcc attcatcaag ccgttctggc
    15241 agagatgaag aatttgatcg aatcatcgta gcgacacggc gctgccgtgt cgcttttttt
    15301 attacggccg ccgcaccctg ttgctaacaa tggatgcccc tgaagaaggc gatggggcga
    15361 tctcgccttc gattggctca tggccgtcgt aatgcatcaa ctgcggccac ttccacatga
    15421 tcagcagtgt gccgatgata cagccaatgc cgccgctgat gaccgcaaac gatgcgccga
    15481 aaaactgcgc caccgaacct gcttccattt caccaagctg cggcccaccc tgaaaaaata
    15541 tctggttgat gctggtcatg cgtccgcgga tatgatcggg cgtttgcagt tgtcgaatcg
    15601 tattgcgaat gatcgtgctg actccatccg ctgcacctgt ggtggcaatg gccagccacg
    15661 ccacccagaa agatctggtc aggccgaaaa tgaccgtggc aatcccaaac acgatgacag
    15721 cacccatgaa catgggaccc tgcctgcgaa tcaccttgac ctgtgagatg gccagcgcca
    15781 ccgtcacagc acccaccgct gaagccgcag agagatagcc atattccacc acccccacct
    15841 tcaaaacggt cgtggcaatg atcggcatca gcgtattcgc ggaagcaaag aacgttgcca
    15901 caaaatccag cagcatcgaa gagaggatga tgggtttgcc aagaatgaac cgaatgcctt
    15961 cccgaatcgc atccatgctg atgcctgccg atttctcggc aaacgattgc ggaacatctc
    16021 cgatcataaa cagggcgaac agcactgcgc cgaacgagat cgcattgaaa tagtaaaccg
    16081 cctcctgccc agcccaggca atgacaaacc cgctcaaggc gggtccaagc acagagccaa
    16141 actggaatga ggtgaaggtc atgctgaaag cgttgggcag atcctctttt ggcacgaggt
    16201 ttggaatcag agcctgacgt gaggggccat cgaaggcgat ggcaatggct tgaatggcgg
    16261 tgatgacgta gatgtaccag atggtgacct gcccaaattg ggtgagcagg ccgagggtca
    16321 gggcgagaag cgccgcagca ctttgagtca cgaacatcac tttgcgcctg tccactgaat
    16381 cggccagtgc gccgccgatc agcgaaaaaa cgatcacagg gagaatgcgg gcaaggccga
    16441 ttccgccaag ggcgatcggg tttttatcca gattgctgac atgccaaaat aaagcccaga
    16501 gttgcatctg ggttcctgtg atcgagatca gttgtccgag ccagaggtag aaaaattttt
    16561 tgtgtttaag agaaggcggg atatgcattt gtatttccag tgatcagcac agaaggcagt
    16621 gaacagtttt caggtatcac tgcacactgg ctcctgttca tttatgttcc ccaatcgcgg
    16681 agatataaaa agtcgtagcc aatggtgcgg ttgctgactt tggcgatcgg cggctgcatt
    16741 cgtttgaatt gattgcggcg aatgcgtgtg gtgacggttt tgacgaaatc ttcggcgaag
    16801 cccacttcga tggcttcctg cggactgtag cgttggtcta caagcaggta gagaagttta
    16861 tcggcttctt cataggtgaa cccgagttct ttttcatcgg tctggtctgc ccacaggtcg
    16921 gctgacgggg gtttttcaag gatgggagca gggatgttca tagcccgtga aagctggcgg
    16981 acctgggttt tgaacaagtc accgatgggg ttgagggcat tggcagaatc gccaaacagg
    17041 gtgctgtatc ccaggagaat ttcagttttg ttgctggtgc caaccaccag tcctttgaat
    17101 acttcgctct ggtcatacag cacgatcatg cgttcgcggg ccatgatgtt gcctttgcgc
    17161 atgttcgaaa tatctggatg aagtttgaag agcggttcaa ccatgtcggt gatctcgatg
    17221 gtcttgctct gcacaccgag ctggtcaatg agaagttggg catgctccag cgaatcttta
    17281 ctggaggctt tgtagggcat gcgcacggca aggacattct gcgggccaag ggcttcggct
    17341 gccaatgcac acgaaagcgc cgagtcgaca ccacctgaaa gaccaaccac tgcacgagtg
    17401 agtcccactc gcgtgatctc agatttgatg aaaccagtaa ggatttcacg cgcaaggtct
    17461 gcattgatgg tgaggttgag gtcagtcacg ggagagaatc ctgtccaatt cgtttcgaat
    17521 gagggcggtg cgttcatcgc gtaaaagcgg aaggcgcgca cgtgtacggc ggagttgatt
    17581 cagatccaac tgggtgtagg tgatggcttc ttcattgtag gggccgtgag caagttcttc
    17641 gccattcggg tcaaaagcgg tggccccgcc ccagaaatga agcccatctt catagccgac
    17701 acggttggca tgggcgacaa aggaagtgaa catgctggcg taggccttgg taacacgttc
    17761 cacccagcgg gcagattcga gtttctcctt gtcgttcaag ccacgccccg gtgaagcaga
    17821 cgagaacagc atgatatctg caccatcaag ccaaagcagg tacggaggcg aggcatgcca
    17881 gaagtcctcg cagatgagca tgcccatgcg cccaaaacgt gtgttaaacg cacggaccga
    17941 gtcaccccac gcaaaaaagc ggccttcatc aaaaaggccg taggtaggta ggtagacttt
    18001 gtgatggaca tgaatggctt tgccacctga aaggtatgcc gaggcaatgt aaaagcggtg
    18061 acgggagtct tcgtctacga atccaatgac caggtccaga tcgtgagacg ctttgaggag
    18121 atgtttgaag atcgggtcat cctctgtcgg cttgtgtgcg gccgaagcga ccaggtcctg
    18181 caatacatat ccagttaacg aaagttcggg gaataccaat aaatcggtct tttcagcttt
    18241 ggcctgttcg atgtactcga gatgcttggc aaggtttgcc tccacatctc caagccgggt
    18301 gttgatctgg gcgagggcaa ggttgagttt cattggacga atcttaccac gcttgtctga
    18361 aaataaaatg ccccaagaga tggacaagat tgaaaaaaa
//
LOCUS       DAOQJC010000079        18351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1415_length_18351_cov_10.420365, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000079 DAOQJC010000000
VERSION     DAOQJC010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18351
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1415_length_18351_cov_10.420365"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PLE39_12400"
     CDS             complement(<1..479)
                     /locus_tag="PLE39_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69178.1"
                     /translation="MKTIKNILLVLSTGYIFVFFSEHLFWARIRPEDTLKDWVGAWIA
                     YSLMAYVFLILITHFKVRNIWPLFLAGAAFGWLAEGLVVQTTYEMLPLSISFTGLAWH
                     ALITIWIGWYALQRSFHSPASFPTWKLVAAIGACSALWAIAWWLEPDGGVASVAEYA"
     gene            complement(613..1365)
                     /locus_tag="PLE39_12405"
     CDS             complement(613..1365)
                     /locus_tag="PLE39_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69179.1"
                     /translation="MKQKTLIPGLVLLLSIIACNLPSGNPPATQTPIVQIITATPADT
                     PVPTFTLIPTETALPTFTPTPTIPIAWPLDKGVNCRYGPSTDWVATSSLLVGQTATIQ
                     GKNGDASWWYVTTPEDPGNPCWVSASVTVTAGNLANLPVIKSPTASVTGVDVKVDPKE
                     INLPGCLGPVQPIKINGSITVNGPTKVDWYFETQQGGSMGTYTLEFDKADTKKVDANF
                     NPNPAAGDFWVKLITITPNGKVAETKYKINCP"
     gene            1502..2242
                     /locus_tag="PLE39_12410"
     CDS             1502..2242
                     /locus_tag="PLE39_12410"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNE69180.1"
                     /translation="MDPVLHDQLINEVLHHTQTDPHKRASGLSDFILGAQDGLVNVLG
                     VVLGIAAATNDARIVLVAGLATTFAESISMGAVAYTTTLADADLYQSEREREYRHILE
                     APNLEIKEIRDIYASKGFKGELLDHIVNTITANQDVWVAVMMAEEHNLAPVDRKTAVR
                     AAIVVGVSAILGSLVPLFPFMFWSVSVSMWLSILVTALVLFGIGAYKASVTVGKPMKS
                     GLEMAVIGTVSALAGYLVGVILKVPPVP"
     gene            complement(2244..3137)
                     /locus_tag="PLE39_12415"
     CDS             complement(2244..3137)
                     /locus_tag="PLE39_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69181.1"
                     /translation="MSDYAVPQHIFAETVATHMQRNNLIPGVLILKGDQSIGVIPRHK
                     MFERLGKRYGVELFLRKPVGELETELGITPFILKHHLPINVAVKLALSRSEGNIYDPI
                     VVEFEDGSRSLLDMYVLLLTQSQLSTNLSGIISSLNNIETILANGMAGASTLNLILES
                     MGSVVPYHHARILLQYQQSISELAGLKDTVQTSHEPLERNDIYRSILSINQPVSLEDV
                     RIVPTWKGSEFPSITRSWMGIPLSIQNGIVGLVALSRFTYSPFTNNEKELGLVFTRYI
                     STLLSDLTNRTKRARSYEKLF"
     gene            complement(3302..3622)
                     /locus_tag="PLE39_12420"
     CDS             complement(3302..3622)
                     /locus_tag="PLE39_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69182.1"
                     /translation="MTLVLIYTLIAGVIAAVTAPIPGTSLLLTGLELYMLVHLAKTNG
                     YKLGMKEIGYTAAAIYGLSTLLQDVALELLTFVPVIGWGAEVVVAVIFVFFLGMLANL
                     YFKK"
     gene            complement(3619..4116)
                     /locus_tag="PLE39_12425"
     CDS             complement(3619..4116)
                     /locus_tag="PLE39_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69183.1"
                     /translation="MATIIFNGKSYNSLEEMPEKERLAYEQMMGIFADKNGNGIPDFM
                     EGDLVQKVMAVNSTHVNINANGQTYQNLDDLPPELRQRVDKAFQVMTNFGIIPGVPEE
                     LKMNNQQGNREPMAQSKPFVSREYTPAIQEESGTSIFPWIIGGIMLVLCLAMAAFGVL
                     YYIAR"
     gene            complement(4127..4438)
                     /locus_tag="PLE39_12430"
     CDS             complement(4127..4438)
                     /locus_tag="PLE39_12430"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNE69184.1"
                     /translation="MQQWLYQLKPSRLEMVTDGPTPEEAEVISRHFAHLQELSRNGVM
                     IMVGRTQNNDESTFGICIFEAEDESAARTIMESDPAVGAGVMRAVLYPYKIALMKGQP
                     E"
     gene            complement(4455..4928)
                     /locus_tag="PLE39_12435"
     CDS             complement(4455..4928)
                     /locus_tag="PLE39_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69185.1"
                     /translation="MRKRAMVMLGFFAGFLLFFLPGILLVNTPGFAPLGGILLGLAVL
                     MLVINIGYSIMGMIQPLWVRQVLQSGTEAKAIIIKNNAITGIGGYKGSDIWLDLPVRV
                     QPANGSAFEAQMKCRLTQTLMLREGSEVTVRYDASNKTKVALVGDARTDMMTKYM"
     gene            complement(4960..5202)
                     /locus_tag="PLE39_12440"
     CDS             complement(4960..5202)
                     /locus_tag="PLE39_12440"
                     /inference="COORDINATES: protein motif:HMM:NF040441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PF20097 family protein"
                     /protein_id="HNE69186.1"
                     /translation="MTDEKVCPKCKGAMTQGRIMKYNEYTTHNRYLYVFAPDGEPGPD
                     LSKMFSASPMSAARKGLVAFCCDQCGFVEFYGQVIP"
     gene            complement(5273..>7240)
                     /locus_tag="PLE39_12445"
     CDS             complement(5273..>7240)
                     /locus_tag="PLE39_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE69187.1"
                     /translation="YGATDIFANLTFAIEKGARLGLVGPNGVGKTTLLRIIVGEDDAS
                     TGNVSRNRGVRIGYLSQEADFKMEGTLWDACHSVFEELIQKQEELHKLESQMATNPDV
                     IERYGKLQEDFERRGGYTYETRIKQVLTGLGFDAEDYSLSLDHLSGGQRTRAFLSRLL
                     LSDPDMLLLDEPTNHLDIRAVEWLEGYLSQWEGAAVIVSHDRYFLDKASNVILEMLPG
                     AYEIYNGNYTAYLKQREERITRRQEVFESEKEKLLKEVEYIKRNISGQNTQQAKGKLK
                     RLSRIVQAVEQIGFEAALNQKWSETAEDVHVTTSNFGVEEAERRVRALRSPVRTLPHL
                     HLRLGSEKRSGDLVVRTKDLEVGYPGKMLFKTPDIELRRQDCAALIGPNGAGKTTFLK
                     TILGQLEPLAGEVVLGASLQIGYFAQAHEGLDPNRTVLQEIDSVMPHWLPGQIRDYLG
                     KYLFSGDDVYKKVDMLSGGERGRLALAKLALQDTNLLLLDEPTNHLDIPSQEVLEAVL
                     EDYAGTILLITHDRYLVDAVATQIWEIDVDGSHLTAFNGTYSQLRAEREKQEYLAFMA
                     AQGAGETSSISNVKRAPSNVKTKEERRKIAQLQELENRIAELEKQLADIGSQLENPPS
                     DAGEVVKLSKAYESTQKEMDEKLAEWETLQG"
     assembly_gap    7241..7340
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7341..7840)
                     /locus_tag="PLE39_12450"
     CDS             complement(<7341..7840)
                     /locus_tag="PLE39_12450"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69188.1"
                     /translation="MKPPEVIITERLTLRKPRMDDAPAVFEGWARHPEVTRFLTWRPH
                     TCVEQTQELMKRSMAAWDGETNFRYLLEIRESHALAGMIELRMEPYKMSFGYTGAFAQ
                     WGKGYMTEAVCASIVWAFEQPTIFRVYATTDVENIPSRRVMEKAGMQCEGILRKDSMH
                     PNLSNVP"
     gene            complement(7866..9029)
                     /locus_tag="PLE39_12455"
     CDS             complement(7866..9029)
                     /locus_tag="PLE39_12455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE69189.1"
                     /translation="MKIRLQLTIFVFLRTILNTAHRMVYPFLSVFARGLGVDIASMSL
                     LMTARSLIGSASPLFAPIADRRGRKFGMLAGISIFTLGMGIVAIHPSYWTLAAALILA
                     VLGKYMFDPAMQAYFGDRIPYEQRGTALAVTEMAWSMAFIIGVPAMGFLISKFSWSAP
                     FPVLAALGALVFFIIWRIIPHEDEHHEPVANSREGYSAVFKSIPALAGISIAMWASAG
                     NELVNVIFGVWLEDSFGLKIAALAGASAVIGISELSGEGLVALTTDRLGKPLALTIGL
                     VGNAVASLLLPIIGTTQIGALIGLFFFYITFEYIMVSHIPMMTELVPSARATMLSLNV
                     TGHAVGRALGAFLAAIIYQRFGFVPVALLAVIFNIAGLLALKRMQRQETDRAR"
     gene            complement(9071..9676)
                     /locus_tag="PLE39_12460"
     CDS             complement(9071..9676)
                     /locus_tag="PLE39_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69190.1"
                     /translation="MLKTKSPLLFFLLTIGAIALLALFGPEEQSLGSNVRIVYLHGAW
                     VLTAELAFAAAALTGLLGLLLKNDQLHAWSAALGRTGIVYWLTYLPLSLFAMQANWNG
                     LFLAEPRFRLAMVFAITGILLQAGLWLFNISWLTSLGNIIFIVVLRVIFSTAQNIMHP
                     PPSPIFNSGNYVIIGFFVGLNLLAWLAAYFLAKWFLQLKAN"
     gene            9802..10671
                     /locus_tag="PLE39_12465"
     CDS             9802..10671
                     /locus_tag="PLE39_12465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNE69191.1"
                     /translation="MEYWPLIVIVLQLVFLEGILSIDNAAVIGALVSPLPENEDVPWP
                     GKLKKLGTVLHPFLGFQRMAALRVGLLGAYLGRGAMLFLASFLIHNSWIKLIGAVYLI
                     HLAFDNLEDMTGDSEEEGGLSPIKVRSFWATVMTVEIMDLIFSIDNVVAAVSLSDKIW
                     VVMLGVGIGILTMRYAAGIFSYAVQHEPILKQAAYILVLNIGVELILDQVWHVEIPDL
                     LRFGISVATIALAIAYAHFPFLQKFRFVLVWLAKGIGIVNEFVDWLFIPFKELFNLIA
                     SLFVRPAGTHPAE"
     gene            complement(10750..11193)
                     /locus_tag="PLE39_12470"
     CDS             complement(10750..11193)
                     /locus_tag="PLE39_12470"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69192.1"
                     /translation="MPIIKTPKTREEFKAYYSLRYKVLREPWGHPKGTEKDDYEPISE
                     HFMAVDEKTGEVLGVIKLFEKAPGVGQVSHLAVAQEHQHKGIGKFLIVEIEKRAHEHG
                     FKTLGAMARVTATAYFEKYGYHIAGIPTPHLGTTHLVWMEKELSS"
     gene            11478..>14383
                     /locus_tag="PLE39_12475"
     CDS             11478..>14383
                     /locus_tag="PLE39_12475"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNE69193.1"
                     /translation="MAIEKKEDMLQENAWHALNAEQVLRDLEVHEEGLTSAEAEKRLA
                     HYGPNQLREAPRPGFLALLWGQLNNFVVILLIVASVISALLGDYIEAAAIMAIVVLNA
                     VLGIVQEQRAEQALAALKKLAAPDARVLRDGAHTSVPSYNLVPGDIVFLEAGNFIPAD
                     LRLLEAVNLRVEEASLTGESLPVQKNAATVLDKNVPLGDRKNTAFMGTVVNYGRGRGV
                     VTSTGMRTQLGLIATMLQNVETEETPLQRRLDQLGRSLSIGSLILVAVVFIVALFNYT
                     EISGLFSSPLDYLKEFASEITEVFIIAISLAIAAVPEGLPAVVTISLALGMREMVQRH
                     ALIRKLSSVETLGSATTICSDKTGTLTQNEMTVTRVWADNQFVHITGTGYAPNGEFQM
                     DGKTVDVTKYPAILSALWLGILNNDATIETTGETESQRTYRIVGDPTEGALLVAAAKA
                     GAIHVDIDEAYPRENEVPFDSERKRMITIHDVRSPKPTDPSPFYDEKHKDWDVIAVKG
                     APDIVLELCTKYLGMDDKPREMTKEAKERVLAANDAMTKDALRVLGFAYRVEKDVPDN
                     PENIKADELEKDLVFVGLLGMIDPPRTEVKPALEKARHAGIRTVMITGDFPNTAKAIA
                     ESISLLRPGKNVMTGAQLDAIDDNELKRIIEDTDVFARVSPEHKMRIVDALQANDEIV
                     AMTGDGVNDAPAIKRADIGVSMGITGTDVAKETADMVLTDDNYASIVSAVEQGRIIYS
                     NIRKFVFFLLSSNVAEIMIIFLATLAGLPAPLTAIQLLWLNLITDGAPALALAMEKGD
                     PDIMDQKPRAKNEPIVNRSMGIGIIVQTIAQTSAVLGAFVMGLAWHLEAGAVLPSGMN
                     VLSFVLSHDWRGVDVQTAETMAFVTLSLAELFRAYTVRSERASIFQIGFFSNKYMQYA
                     VGISIALLLLVCAVPFLQPIFNTHFLSGREWGLVLGLALMPAVAEEITKFFLRR"
     assembly_gap    14384..14483
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14516..15658
                     /locus_tag="PLE39_12480"
     CDS             14516..15658
                     /locus_tag="PLE39_12480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNE69194.1"
                     /translation="MFRFQGEPFTPEQKTKFHFFASILALALIAVVVAVLFQPLILPR
                     MDFYNELWGPSYLLVHRQSPYDTASLNPVLPAVWFPMAIGFFFPLGWLSEDAALQAWF
                     FFNIVEICIVVFLVQGKDRSLYNTAALALLCFFFPFVLNHFNLGQFSITILLCLLLSA
                     HWIGKYKNWPSAFFLALALAKPQLGILAVIGLAVHQFRQRGFVGMFLFGAQTLLMALL
                     MSLPLFIAHPAWIPDWIASMAKNYTWQHPSLFSVFQQMLGRWGLVPWAAIAAGVLAFT
                     FLLWKKIPPFAAMLWSLALTTLVTPYVWSWDFVLLLPLWMFSFTKANWKWKILLLVSY
                     LIAWYGMALVQMQAGSQNQSFWWVPLWFIGTLVPAWRMGEVKYFDS"
     gene            15689..16972
                     /locus_tag="PLE39_12485"
     CDS             15689..16972
                     /locus_tag="PLE39_12485"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNE69195.1"
                     /translation="MPRFIRVTFFLALLSLTLACGLFQSAPSVRDPLWVYANPNFSPD
                     YASIQSSESGLVTYQSGASNELPISLITLNPASGEEIFTPTPNDELVIFEGVAAGRAI
                     FVGRVSDQVNEDGDVLYPIIALDPSTGLEVWRYDGSVFESGIPRTEKYLFVSKRYSQI
                     DVVDLMNGEVVASFTKEYDAAELFDLNPGWIRYMYTDTAIYSLSPAGVLRRYDLPTLT
                     LQSTLNLDIPHYLDAFFIEGDRLYVYSAEVLDSDLNLLAFDLASGTHLWELRGMRGST
                     VGLKFYNGFGYVDTVNGPSKIDLSNGQVLWSVSVQLAPFFIGDDSNSVFLATQDGLLT
                     AYDSTSGKELWTYRTGQPNSINLSAVNGVAFITTAEDLAAFESFFVPNQLDAVDIATG
                     KALWRYKNPYVTLPIGTDNAFIVGYYGGITALPAK"
     gene            complement(17028..17453)
                     /locus_tag="PLE39_12490"
     CDS             complement(17028..17453)
                     /locus_tag="PLE39_12490"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HNE69196.1"
                     /translation="MNCPKCNTELAKKYYKGMIEVDSCPNCRGMWLDFNELDKLEDVA
                     FDDDSRKGSLIHHQAKTEFPCPHCGAAMDEFQYRLHDLKLDYCAENDHGFWLDAGEDE
                     RVIAAMNQRAQDIQRKTSAESDWRQTLKDMHAFLRKWKP"
     gene            complement(17518..>18351)
                     /locus_tag="PLE39_12495"
     CDS             complement(17518..>18351)
                     /locus_tag="PLE39_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNE69197.1"
                     /translation="KIRLDVLLVERGLAESRAKAQALIMAGQVRVNDQVTLKPATAID
                     TKSALTVDTGPRFVSRGGEKLDAALERFGIDVKDFICADVGASTGGFTDCLLQRGAAK
                     VYAIDVGKGILHWKLRNYARVVVMEETNARHVESLPEAVSLVTVDASFISLKILLPVV
                     KKWFSFFPLSSFNDDEREKKKEGIVALIKPQFEAGKKDVARGDGVIRDPEIHRQVLLD
                     VLTFAKQEGFNLRGLIKSPLLGPKGNAEFLVWLDFQENGRSVEDLVDEAMAESTSNLT
                     E"
BASE COUNT         4142 a         4588 c         4721 g         4700 t
ORIGIN      
        1 ctggcatatt cagccaccga tgcgaccccg ccatcaggtt ccagccacca ggcgatggcc
       61 caaagcgctg aacatgcccc gatggcagca accagtttcc aggtcgggaa agaagcgggg
      121 gagtgaaacg acctttgaag agcataccat ccgatccaaa tcgtgatgag tgcgtgccat
      181 gccaatccag taaatgaaat tgagagtggc agcatttcat aggttgtttg cactacaagg
      241 ccttctgcaa gccagccaaa agccgcgcct gcaaggaaca gcggccagat atttctcacc
      301 ttgaaatgtg tgatgagaat caaaaagaca taagccatga gcgaataggc gatccatgcg
      361 ccgacccaat ctttgagcgt atcttcaggg cggatgcgtg cccaaaaaag atgctcggaa
      421 aagaatacaa agatgtagcc ggtggaaagc acaagcagga tgtttttgat ggtcttcatg
      481 aaatcaatac aggttttgcg catgaaaagt tgccgataca aaaaagcgac ccgtttgaaa
      541 cggaatcgct ttttgttgtg ttccttgttt tcttttgaat gctaaaaaag gagctttggt
      601 atcaagacca ttttatgggc aattgatctt gtatttggtt tcagctactt ttccattggg
      661 tgtgatggtg atgagtttta cccagaaatc tccagcagcg gggttgggat tgaagttggc
      721 atccactttt ttggtgtcgg ctttgtcgaa ttcaagggtg taggttccca tggaaccgcc
      781 ttgctgggtt tcgaaatacc aatccacttt ggtcggtccg ttgaccgtga tgctgccgtt
      841 gatcttgatg ggttgcacag ggccaagaca gccaggcagg ttgatttcct tcgggtcaac
      901 tttcacatcc accccggtga cggatgcagt gggtgattta atgacaggca ggttggcaag
      961 gttgcctgcc gtaaccgtta cgctggcaga tacccaacaa gggttgccag ggtcttccgg
     1021 ggtggtgacg taccaccagc ttgcatcacc gttcttgcct tgaatggtgg cggtttgccc
     1081 gaccagcagg gaactggtgg ccacccaatc ggtgcttggc ccgtaacggc agttgacgcc
     1141 tttatcaagc ggccaggcaa tgggaatggt tggagtgggc gtgaaggttg gcaatgccgt
     1201 ttcggttggg atgagggtaa aagtgggaac aggagtatct gcgggtgtgg ctgtaatgat
     1261 ctgtacgatg ggagtttgtg tggcgggcgg atttcctgac gggagattgc aggcaatgat
     1321 ggaaagtaat aaaacaagcc cggggattaa agttttctgc ttcataaaat aacttctcct
     1381 tttcatggaa aagaatataa acaacatggc agaaagacac aatgccccat tggttttgtt
     1441 gatgcaagac tggtgacatt tgtcaattct cagggctgtg ttgtctgagt atcattgttg
     1501 tatggaccca gttttgcacg atcaactcat aaatgaagtt ttacatcaca cccaaactga
     1561 cccgcacaag cgggcttcgg ggctttctga ttttatttta ggcgcacaag atggattggt
     1621 taatgtgctt ggcgtggtgc ttggcattgc cgccgctaca aatgatgcac gtatcgttct
     1681 ggtggcgggg ctggccacca ctttcgctga atccatttca atgggggcag tggcctacac
     1741 caccacactg gcagatgccg acctgtatca aagcgaacgt gaacgggaat atcgtcatat
     1801 tttggaagcc cccaatcttg aaattaagga aatccgcgat atttatgcca gcaagggctt
     1861 caagggagaa ctgctggatc acatcgtaaa taccattacc gcaaatcagg atgtgtgggt
     1921 ggccgtgatg atggccgaag aacataacct tgccccagtg gaccgaaaaa ccgcagtgcg
     1981 cgcggcgatt gtcgttggcg tttccgcgat ccttggttca cttgtgccgt tattcccctt
     2041 tatgttttgg tcggtatcag tgagcatgtg gctgtccatt ttggtgacag cccttgtgtt
     2101 attcggtatt ggcgcctata aggcatctgt cactgtgggc aaacccatga aaagcggact
     2161 tgaaatggcg gtcatcggaa ccgtcagcgc gttggctggt tatttagtgg gtgtcatcct
     2221 caaggttccg cctgtgccat aggctagaag agtttttcat aagagcgagc cctttttgtt
     2281 ctgttggtca aatctgacag aagagtgctg atgtagcggg tgaacaccag tccgagttct
     2341 ttttcgttgt tggtgaaagg ggagtaggtg aatcttgaaa gcgccacaag cccaacgatc
     2401 ccattttgaa tcgagagcgg aattcccatc catgaacgtg tgatagatgg gaattctgag
     2461 cctttccatg taggtacgat acgcacatcc tccagactca caggttgatt gatggaaagg
     2521 atcgagcggt agatgtcatt tcgctccaga ggttcatggg atgtttgaac agtatctttc
     2581 agaccggcta attcgctgat actctgttga tattgcaaga gtatccgtgc gtgatgatat
     2641 ggcaccaccg aacccatgct ttcgagaatc aggtttagag tgcttgcacc tgccattcca
     2701 ttggcaagga tggtctcaat attgttgagt gaactgatga tgccgcttag atttgtcgaa
     2761 agttgagact gcgtaagcag taacacatac atgtccagca aagaccggct tccatcctcg
     2821 aactcaacca cgatggggtc ataaatattc ccctctgaac ggcttaaagc aagtttgaca
     2881 gccacgttga ttggcaggtg atgtttgaga atgaatgggg tgatgcccag ttctgtctcc
     2941 agttcaccaa cgggttttct gagaaacaat tccactccat atcttttgcc cagtcgttcg
     3001 aacattttat ggcgcggaat gactccaatg gattgatcgc cttttagaat aagcacccct
     3061 gggatcaggt tgttacgttg catgtgcgtt gccacggttt ctgcgaatat atgctgcgga
     3121 acggcataat cagacatggg caaatcccca atgcagacag gaatggggat ttgcccggta
     3181 gggaatactt tgaaataatg gggaatggtt tggagttctc tcatggatgg tgagagaatt
     3241 gtatggcgca gggtttaatt gggtttgaaa agcaggttaa cggtcggtaa agaaacgatt
     3301 cctacttttt gaaatacaaa tttgcaagca tccccaaaaa gaaaacgaag atcacagcta
     3361 caaccacttc cgcgccccag ccaatgactg ggacgaaggt cagcaattcc agggccacat
     3421 cttgtaacag ggtggagagc ccataaatag ctgccgccgt ataaccgatt tccttcatgc
     3481 ccagtttgta tccattcgtt ttggcgagat ggacaagcat gtaaagttcc agcccggtca
     3541 acagcagaga tgtgcccggg atcggcgcag tgacagcggc gatgacgcca gcgatcaggg
     3601 tgtaaataag aacaagggtc atcttgcaat gtaataaagc acgccaaaag ccgccattgc
     3661 cagacacaac acaagcataa tgccgccaat gatccacgga aaaatactcg tcccgctttc
     3721 ctcctgaatg gcgggggtgt attcacgcga aacaaaaggc ttggattgtg ccatcggttc
     3781 gcggtttccc tgctgattat tcattttcaa ttcctcgggt acaccgggga tgatgccaaa
     3841 attggtcatc acttgaaacg ccttgtccac acgctggcgc agttcaggcg gaaggtcatc
     3901 aaggttttgg taggtctggc cgttggcgtt gatgttgaca tgggtggagt tgaccgccat
     3961 cactttttgc accagatcgc cttccataaa atcaggaatg ccattcccgt ttttgtctgc
     4021 aaagatgccc atcatctgtt cataagccag acgttctttt tcgggcatct cctcaaggct
     4081 gttgtagctt tttccgttaa atatgatcgt tgccattgaa actcctttac tctggctggc
     4141 ctttcatcaa ggcaatttta tacggatata aaaccgcgcg catcaccccg gcgccgacag
     4201 ctgggtctga ctccatgatc gtcctcgccg ctgattcatc ctccgcctca aaaatgcaaa
     4261 ttccaaacgt ggattcgtcg ttgttctgtg tgcgtcccac catgatcatc acgccgttgc
     4321 ggctgagttc ctgcaaatga gcaaagtggc gcgaaatgac ctcggcttct tcgggcgttg
     4381 gcccatcggt gaccatttca agacgggatg gtttaagttg gtaaagccat tgctgcatgg
     4441 ataccttcct tttgttacat atactttgtc atcatgtcag ttcttgcatc gccgacaagc
     4501 gcaacctttg tcttgttgga tgcatcgtat cggaccgtga cttcactgcc ttcgcgcagc
     4561 atcagggttt gagtgaggcg gcatttcatc tgggcttcga aggctgaccc attggcaggt
     4621 tggacacgca ccggcaaatc cagccagatg tcactgcctt tgtagccgcc gatccctgtg
     4681 atcgcattgt tcttaatgat aatcgctttc gcctcggtgc cgctttgcaa gacctgcctg
     4741 acccacaaag gctggatcat tcccatgatc gagtacccga tgttgatcac gagcatcaac
     4801 acggccaacc caagcaaaat gccgcccaga ggggcaaacc cgggtgtgtt gaccaaaagt
     4861 atgccgggca gaaagaataa caaaaaacca gcaaagaaac caagcataac catggcgcgt
     4921 tttcgcattt gtcttttctc ctaaaatttt tgagatcgat caggggatga cctgcccgta
     4981 aaattcgacg aatccacatt ggtcgcaaca aaacgccacc agccccttgc gtgccgccga
     5041 cattgggctt gcagaaaaca ttttcgatag atcgggtcct ggctcaccat ctggcgcaaa
     5101 gacatataga tagcggttgt gtgttgtgta ctcgttgtac ttcatgattc gcccctgtgt
     5161 catcgcccct ttgcattttg gacatacttt ttcatcagtc atttgggtct ccttttgaat
     5221 gtccccattc taaggacccg aaaagaaaat caatatgcct caaaagtatg aactacccct
     5281 gcagcgtctc ccactctgcc agtttctcgt ccatttcctt ctgtgtggat tcgtaagcct
     5341 tgctcagttt taccacttcg cccgcatcag atggcggatt ttccaactga ctgccgatat
     5401 cggccagttg tttctccaac tcggcaatgc ggttttcaag ctcctgcaac tgcgcgatct
     5461 ttcttctttc ctcttttgtc ttgacgttcg aaggcgcgcg tttgacattc gagatggaac
     5521 tcgtttcacc tgcaccttgt gccgccatga aagccagata ctcctgcttt tcccgttcgg
     5581 cgcggagttg tgaataggtg ccattgaagg cggtcagatg tgagccatcc acatcaattt
     5641 cccagatctg ggtggcgacg gcatccacca gataacggtc gtgggtgatg agcaggatcg
     5701 ttcccgcgta atcttccaaa accgcttcca aaacttcctg cgacggaata tcgaggtggt
     5761 tggtgggttc gtcaagcaaa agcaggtttg tatcctgtaa ggccagtttc gccaatgcca
     5821 gccttccgcg ctcaccaccg gagagcatat ccactttttt gtacacatcg tcgccgctga
     5881 aaagatactt gcccagataa tcacggatct gccccggcag ccaatgcggc atgaccgaat
     5941 cgatctcctg caacaccgtg cggttcgggt cgagaccttc gtgcgcctga gcaaaatacc
     6001 caatttgcag actcgctccc agaaccactt caccagccaa cggttcgagt tgaccgagga
     6061 tcgtcttcag aaatgtcgtc ttgcctgcgc cgtttggtcc gatcaaagcc gcgcaatcct
     6121 gccgacgcag ttcgatatcg ggagtcttga aaagcatttt gcccgggtag cccacttcca
     6181 gatcttttgt gcgcaccacg agatcacccg aacgtttttc agaaccgagg cgcaaatgca
     6241 gatgcggcag cgtacgcaca ggcgagcgca aggcgcgcac acggcgttcg gcttcttcca
     6301 cgccaaagtt cgatgtggtc acatgcacat cttcggcggt ctcgctccac ttttgattca
     6361 acgccgcttc aaaaccgatc tgttccacag cctgcacgat gcgcgaaagc cgcttcaatt
     6421 ttccttttgc ctgctgggtg ttctgccccg aaatattgcg cttgatgtat tcaacttcct
     6481 tgagcagttt ttctttttca ctttcgaaaa cttcctgcct gcgtgtgatt cgttcttcgc
     6541 gctgtttcag gtaggcggtg taattgccgt tgtaaatttc gtatgcgccg ggcagcattt
     6601 ccaaaatgac attcgaggct ttatccaaaa aataacggtc gtgcgaaacg atcaccgccg
     6661 cgccttccca ttgactcaaa tatccttcga gccattcaac ggcgcggatg tcgaggtgat
     6721 tggtcggctc atcgagcagg agcatatcgg ggtccgaaag aaggagacgc gataaaaagg
     6781 cacgggtgcg ctgcccaccg gaaagatgat ccaaagaaag gctgtagtct tcggcgtcga
     6841 atcccaaccc ggtcaacacc tgtttgatgc gcgtctcgta ggtgtatcca ccgcgcctct
     6901 cgaagtcttc ctgtagtttc ccatatcgtt cgatcacatc ggggttggtc gccatctggc
     6961 tttcgagttt gtgcaactct tcctgttttt ggatcaattc ctcgaaaacg gaatgacacg
     7021 catcccaaag agtcccctcc attttgaaat ctgcctcttg cgacaaatac ccgattcgga
     7081 ctcccctgtt tctggatacg tttcccgtcg aagcatcatc ctccccgacg atgatccgca
     7141 gcagggttgt tttgccaacg ccattgggac cgaccaaccc caaccgtgcg cccttctcga
     7201 tggcgaaggt caggttggcg aagatatcgg tggcgccgta nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn ggcacattgc tgagattggg atgcatactg tccttgcgca
     7381 ggatgccttc gcattgcatc cctgcttttt ccatcacccg ccgcgaaggg atattttcca
     7441 cgtcagtggt ggcgtacacg cggaagatcg tcggctgttc gaatgcccac acaatcgagg
     7501 cgcatacggc ttcggtcatg tagcctttgc cccattgggc gaatgcccct gtgtaaccga
     7561 agctcatctt gtaaggttcc atgcgaagtt cgatcatgcc tgccagcgca tggctttccc
     7621 tgatctccag caaatagcgg aaattcgttt ccccatccca cgctgccatg gatcgtttca
     7681 tcagttcctg tgtctgttcc acgcaggtgt gtggacgcca tgttagaaaa cgggtgactt
     7741 cgggatgcct ggcccagcct tcgaacacgg ctggagcatc gtccatgcgt ggtttgcgca
     7801 gggtgagtct ttcagtgatg atgacctcag gtggtttcat aaatcctttt ttgtggtgcg
     7861 ggatattatc ttgccctgtc agtttcctgc ctttgcattc tctttagcgc caataaacca
     7921 gcgatattga aaatcacagc cagcaatgcc acaggcacaa acccgaaacg ctggtaaatg
     7981 attgcggcaa ggaatgcacc gagcgcacgt cctacggcat gacctgtcac attcagtgag
     8041 agcatggtgg cgcgcgccga tgggacaagc tcagtcatca tggggatgtg actgaccatg
     8101 atgtattcaa aggtgatata gaagaagaac agcccgatca acgcgccgat ctgggttgtt
     8161 ccaatgatcg ggagcaggag cgaggcaaca gcattgccga ccagaccaat ggtcagcgcg
     8221 agcggcttgc cgagtctatc ggtggtgaga gcgacgagtc cctctccgct caattctgag
     8281 atgccgatca cagcagacgc ccctgcaagc gcggcgatct tcagcccaaa tgaatcttcg
     8341 agccagacgc cgaagatcac attgaccagt tcattgcccg cactggccca catggcgatc
     8401 gaaatccctg ccaacgcagg gattgatttg aagaccgcac tgtaaccttc gcgtgaattg
     8461 gcaacaggct catgatgttc atcttcatgc ggaatgatgc gccagatgat gaagaataca
     8521 agggcgccaa gtgcagcgag gacagggaag ggtgccgacc aactgaattt cgagatcagg
     8581 aatcccatcg caggaacacc gatgatgaac gccatggacc aggccatctc cgtgacagca
     8641 agggcggtgc cgcgttgttc gtagggaatg cgatcgccga aataggcttg catggcgggg
     8701 tcgaacatgt actttcccag cacggcaagg atgagtgctg cggcaagcgt ccaatacgat
     8761 gggtgaatgg cgacgatgcc catgccaagc gtgaagatgg agatgcctgc cagcatgcca
     8821 aacttgcgtc cacgccgatc ggcgatgggt gcaaagagcg ggcttgccga gccgatcagg
     8881 gaacgggctg tcatcaacag ggacatggag gcaatatcca cgccgaggcc gcgcgcaaat
     8941 accgataaga atggatacac catcctgtgg gcggtgttca ggatggtgcg gaggaataca
     9001 aagattgtga gttgtaggcg tattttcaaa atataaactg tagtgcaaga tattatcctg
     9061 cactaccttt ctagttggcc ttgagttgca aaaaccattt tgcaaggaag taggctgcca
     9121 gccatgcgag caggttcaag ccgacgaaga acccgatgat gacgtaattg cctgaattga
     9181 agatcggcga tggcggcggg tgcatgatgt tttgtgcagt ggaaaagatc acccgcagaa
     9241 caacaatgaa aataatgttg cccagtgaag tgagccatga aatattgaac aaccacaagc
     9301 cagcctgaag cagaatgccc gtaatggcaa agaccatggc cagacggaag cgaggttcag
     9361 cgagaaataa tccgttccag ttggcttgca tggcaaagag ggaaagcggc aggtaggtga
     9421 gccaataaac aatgccggtg cggccaagag cggcactcca ggcgtggagc tggtcatttt
     9481 tcaggagcag tcctaaaagt ccagtaagag ccgcagccgc gaaggcaagt tcagcggtca
     9541 atacccaggc gccgtgcagg tacacgatcc gcacgtttga tcccagtgat tgttcttcgg
     9601 ggccaaataa ggccagcaag gcgattgccc cgatggtaag tagaaaaaaa agcaggggtg
     9661 attttgtttt taacatgtca cggattttaa catcctgaac gatgttttta tagtgccttt
     9721 tgtcagttcc atgcggttac ccattgagat aaaattcata cttggtttga aatctaatga
     9781 agtggaaggt tctttattaa catggaatac tggcctttga tcgtgatcgt tcttcagttg
     9841 gtgtttttgg aagggattct atccattgac aatgccgcag tgatcggtgc cctggtttcc
     9901 ccactgcctg agaatgagga tgtgccctgg ccgggaaagc tgaaaaaact cgggacagtt
     9961 ttacatcctt tccttggttt tcaaaggatg gctgcgctgc gggtgggttt gttgggggcc
    10021 tatttgggac gcggcgcaat gctgtttttg gcgagttttt tgattcacaa ctcatggatc
    10081 aaattgatcg gtgccgtgta tttgatccac cttgcgtttg acaaccttga agatatgacg
    10141 ggtgatagcg aggaggaagg cgggctttct ccgatcaagg tgcgctcatt ttgggccacc
    10201 gtgatgaccg tggagatcat ggatctgata ttcagcattg ataatgtggt tgcggctgta
    10261 tcgctttccg ataagatctg ggtggtgatg ttgggggtgg ggatcggcat tttgacaatg
    10321 cgttacgcgg cgggtatttt tagttatgca gtgcagcatg aaccgatcct gaagcaggcg
    10381 gcctatattc ttgtcttgaa catcggcgtg gagttgattt tggatcaggt ctggcatgtt
    10441 gaaattcccg atttgttgcg ttttggaatt tcagttgcaa cgatcgcgct tgccattgcc
    10501 tatgctcatt ttcctttttt gcagaagttt cgctttgtgc tggtctggct ggcgaaaggc
    10561 atcggcatcg tcaacgaatt tgtggattgg ttgttcatcc cctttaaaga attgttcaat
    10621 cttattgcca gcctttttgt gcgccctgca ggtacccacc ctgccgagtg accttgaaat
    10681 ctgtatataa acataacccg ccctgcgaaa gtttgatttc ggggcgggtt atgtttttgt
    10741 ctgtcatttt caggaagata attccttttc catccacacc aggtgagtgg tgcccaaatg
    10801 cggggtggga attcctgcaa tatggtagcc gtatttttca aaatatgctg tggctgtaac
    10861 acgtgccatt gcgccaaggg tcttgaatcc atgttcgtgg gcgcgttttt caatttccac
    10921 gatcagaaat tttccgatgc ctttgtgctg atgttcctgc gcaacggcca aatgagagac
    10981 ctgacccacc ccgggcgctt tttcaaacag tttgatcaca ccaagaactt cccccgtctt
    11041 ttcgtccaca gccatgaaat gctcgctgat gggctcgtaa tcgtcttttt cagtgccttt
    11101 tgggtgcccc catggttcgc gaagaacctt gtaacgtaac gaatagtagg ccttgaactc
    11161 ctcgcgggtt tttggcgttt ttatgattgg catattgccc tccgcaacaa actttctcaa
    11221 ttgactgggt cattatactg ctcaatcatg gcgcccgaac aaaactgttg actcttttcc
    11281 ttgacagtga cttttaccat ggagtacact ggctaaggtt ggggagtagc cacctgttcc
    11341 ccacaggatg gatcgtcgtc aaaccattgg tgtaacatcc accatcggcg gttcgtgcgc
    11401 aaattttgtg cgttggcgag accaccacga attgattcgt ggtggtcttt ttttatcttc
    11461 atttcaggag acgagtgatg gctatcgaaa aaaaggaaga catgcttcag gaaaatgcct
    11521 ggcatgcatt gaatgccgag caggttctgc gtgacctgga ggttcatgaa gagggactga
    11581 cgagtgccga agcggagaag cgccttgcgc attatggtcc gaatcagttg cgcgaagcac
    11641 cgcggcctgg cttccttgcc ctgctgtggg gacagttgaa taattttgtt gtgattcttt
    11701 tgattgtggc ctcggtgatc tctgccctgc tgggagatta tattgaagct gccgcgatca
    11761 tggcgatcgt ggttttgaat gctgtccttg gtattgtgca ggagcagcgc gctgagcagg
    11821 ccctggccgc tctcaagaaa ctggctgcgc ccgatgcgcg cgtcctccgt gatggtgcgc
    11881 atacctctgt cccttcttat aaccttgtac cgggtgatat cgtctttctt gaagcaggga
    11941 atttcattcc cgccgacttg cgcctgcttg aagccgtcaa cttgcgtgtg gaggaggcat
    12001 ccctgaccgg tgagtcgctt cctgtgcaga agaatgccgc cactgtgctt gataaaaatg
    12061 tgccgcttgg cgatagaaaa aacaccgcct ttatgggcac cgtggtcaat tacgggcgtg
    12121 gacgtggtgt ggtcaccagc acaggcatgc gcacccagct tggcctcatc gccaccatgc
    12181 ttcagaacgt tgaaaccgag gagacgccgc tccaacgcag gctcgaccag cttggacgtt
    12241 cgttgagcat cggctcgttg atccttgtgg cggttgtttt tatcgttgca ctcttcaact
    12301 ataccgagat cagcggattg ttctcttcgc cgttggatta cctcaaggaa ttcgcctctg
    12361 aaatcacaga ggtattcatc atcgccatca gccttgcgat tgccgccgta ccggaagggc
    12421 tccccgccgt ggtgacgatc tcacttgcgc tgggcatgcg ggaaatggta caacgccacg
    12481 cccttatccg aaaactgtcc tccgttgaga cgcttggttc ggccacgacg atttgttccg
    12541 acaagaccgg aaccctgacc caaaatgaaa tgaccgttac ccgtgtgtgg gcagataatc
    12601 aattcgtcca cataacaggc accgggtatg ccccgaacgg tgaatttcag atggatggaa
    12661 agactgtgga tgtgacgaaa taccccgcga tcctttctgc gctgtggctt ggtattttga
    12721 acaatgatgc caccatcgaa acgaccggtg aaaccgaatc gcagaggacc tatcgcattg
    12781 tgggtgaccc aaccgaaggt gctttgttgg tggcggctgc caaggctggg gcgatccacg
    12841 ttgatattga tgaagcctac ccgcgtgaga acgaagtgcc atttgattcg gaacgcaaac
    12901 gcatgatcac cattcacgat gtgcgcagcc cgaaaccgac cgacccatcc ccgttttacg
    12961 atgaaaagca taaagattgg gacgtgatcg ccgtcaaagg tgcgcccgat attgtgctgg
    13021 aactttgcac caaatacctc ggaatggacg acaagccgcg tgaaatgaca aaggaagcga
    13081 aagagcgcgt cctcgctgcc aacgatgcca tgaccaaaga tgccctgcgc gtacttggtt
    13141 ttgcatatcg cgtggaaaaa gatgtgcccg ataaccctga aaatatcaaa gccgatgaac
    13201 tcgaaaagga cctggtcttc gtgggtctgc ttggcatgat cgacccgccg cgtaccgagg
    13261 tgaagcccgc actcgaaaaa gcccgccatg caggcatccg cacggtgatg atcaccggcg
    13321 atttcccgaa taccgccaag gccattgccg aatcgatcag cctgctgcgc cccggtaaga
    13381 atgtaatgac cggtgctcaa ctcgatgcca ttgatgataa cgaactcaag aggatcatcg
    13441 aagataccga tgtctttgcc cgtgtatccc ccgaacacaa gatgcgcatc gtggatgcct
    13501 tgcaggccaa tgacgagatc gtcgccatga ccggtgacgg tgtgaacgat gctcctgcca
    13561 tcaagcgcgc cgacattggt gtttcaatgg gcattacagg gaccgatgta gccaaggaaa
    13621 cagccgatat ggtgctcacc gatgataact acgccagcat cgtttcagca gtggagcagg
    13681 gacgcatcat ctatagcaat atccgcaaat tcgtgttctt cctgctttcc tccaacgtgg
    13741 ctgagatcat gatcattttc cttgccactc tggcaggcct gcccgcacca ttgaccgcga
    13801 tccaattgct ctggctcaac ctcatcaccg atggcgcgcc tgcactggca ctcgccatgg
    13861 aaaaaggcga tcccgatatc atggatcaga aaccgcgcgc caagaacgaa ccgatcgtca
    13921 accgttcgat gggcatcggc atcatcgtgc agaccatcgc acagacgagc gccgtgctcg
    13981 gggcttttgt catggggttg gcgtggcacc tcgaagcagg agcagttctc ccgtcgggga
    14041 tgaacgtgtt gtccttcgtc ctctcacatg actggcgcgg tgtggatgtt caaaccgcag
    14101 agacgatggc cttcgttacg ttgtcgctgg ctgagttgtt ccgtgcgtac accgtccgct
    14161 cggagcgtgc ttccattttc cagatcgggt tcttttccaa caagtacatg cagtatgcgg
    14221 tgggcatttc cattgcgctg ctgttgctgg tgtgtgccgt gcccttcctg cagcccattt
    14281 ttaacaccca cttcctcagc gggcgtgaat gggggctggt gcttgggttg gcgctcatgc
    14341 ctgccgtggc tgaagagatc acaaaattct tcctgcgaag aagnnnnnnn nnnnnnnnnn
    14401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14461 nnnnnnnnnn nnnnnnnnnn nnntttttga ttaaaaagtg aagtatgatt cagccatgtt
    14521 cagatttcaa ggcgaaccat ttacccctga gcagaaaacg aaatttcatt tttttgcctc
    14581 catccttgcg ctggcattga tcgccgtggt cgtggctgtt ttatttcaac ccttgattct
    14641 gccgcgcatg gatttttaca acgagctttg ggggccttca tacctgctcg tacacagaca
    14701 aagcccatac gataccgcca gcctgaaccc tgtgttgcct gctgtctggt tcccaatggc
    14761 aattggattc ttctttccgc ttggctggct ttctgaagat gcggccttgc aggcctggtt
    14821 ctttttcaat attgttgaga tttgcatcgt cgtttttctg gtgcagggaa aagaccgctc
    14881 gttgtacaac acggcagcac tggccttgct atgtttcttt ttcccctttg tcttgaatca
    14941 tttcaacctc gggcagtttt ccattaccat tctattgtgc ttgctgcttt cagcgcattg
    15001 gatcggaaaa tataaaaact ggccttcggc gtttttcctt gcgcttgccc ttgccaagcc
    15061 gcaattgggg attctggctg tgattggtct ggctgtgcat caattccgcc aacgtggatt
    15121 tgtggggatg tttttgttcg gcgcacagac cttgctcatg gctctgttga tgagcctgcc
    15181 cctgttcatc gcccaccctg catggattcc cgactggatt gcaagcatgg caaaaaatta
    15241 cacctggcag cacccttcgt tgttttcggt ttttcagcaa atgctgggaa gatgggggct
    15301 ggtgccttgg gcggcaattg cagcgggtgt gctggcattc acatttttat tgtggaaaaa
    15361 gataccgccc tttgcagcca tgctatggag ccttgcgctg acaacgcttg tcactcccta
    15421 tgtctggagt tgggattttg tcctgctcct gccgttatgg atgttttcgt ttacaaaggc
    15481 aaattggaaa tggaaaatat tgttattggt ttcatacctt attgcctggt atggcatggc
    15541 actggtgcaa atgcaggcag ggagtcagaa ccagtctttc tggtgggtgc ctttgtggtt
    15601 tatcggcaca cttgtgccag cctggcgaat gggtgaagta aaatatttcg actcatgacg
    15661 attttatttt ttacaaaagg atgaccatat gccgcgtttt atccgtgtga ctttttttct
    15721 cgcgcttctt tccctcaccc tggcctgtgg actcttccaa tcggccccgt cggtgcgtga
    15781 tcctttgtgg gtttacgcca acccgaattt ctcccccgat tacgcctcca ttcaatcttc
    15841 cgagagcgga ctcgtcacct atcaaagcgg cgcttccaac gaactgccga tctcgctcat
    15901 taccctcaac ccagccagcg gagaggaaat cttcacgccc acccccaacg atgagttggt
    15961 catttttgaa ggagttgccg cgggtagggc catttttgtt ggccgagtca gtgatcaggt
    16021 caatgaagac ggcgatgttc tctatcccat cattgcgttg gatccctcaa caggtctgga
    16081 agtatggcga tacgatggca gcgtgtttga aagcggaatt ccgcgcacgg aaaaatatct
    16141 ttttgtttca aaacgctatt cccagatcga tgtggtggat ttgatgaacg gggaagtggt
    16201 tgccagtttt acgaaggaat acgatgccgc cgaactcttc gacctcaacc caggctggat
    16261 tcggtatatg tacaccgata ctgccattta ttcgctcagc ccggcgggag tgctgcgccg
    16321 ttatgacctg cccacattaa ccctgcaatc cacacttaac cttgatatac cgcattacct
    16381 tgatgcgttc ttcattgaag gagatcgact ctacgtatat tccgcagagg ttctggattc
    16441 tgatttgaac ctgcttgcct tcgacctcgc cagcggtaca cacttgtggg aattgcgcgg
    16501 gatgcgcggt tccacggtgg gcctaaagtt ttacaacggc ttcgggtatg tggatacggt
    16561 caacggcccg agcaaaatag acctttccaa cggacaggtt ctttggtcgg tttctgttca
    16621 attggcgccc tttttcatcg gtgatgattc aaatagcgtg tttctggcca cacaggatgg
    16681 acttctcact gcgtacgatt ccaccagcgg aaaggaactg tggacttaca gaacaggcca
    16741 gcccaactcg atcaacctca gcgcagtgaa tggggtggcg tttatcacca ctgccgaaga
    16801 cctggctgcg ttcgaatcct tttttgttcc caatcaactt gatgcggtgg atattgccac
    16861 aggcaaggca ctctggcgct acaaaaaccc atacgtgacc ctgcccatcg gcacggacaa
    16921 tgccttcatc gtcgggtatt acggtggcat caccgccctg cctgcaaaat aaaatccccg
    16981 ctctttttga ggtgtgatgt cagggcgtag gaaaaaaata aagacttcta gggcttccat
    17041 tttcgcagga aggcgtgcat gtccttgagg gtttgtctcc agtctgattc ggcgctggtt
    17101 tttcgttgaa tatcctgtgc gcgctgattc atcgctgcga tcacgcgctc atcctcgccc
    17161 gcgtccagcc agaagccgtg atcgttttcg gcacaataat ccagtttcag gtcgtgcagg
    17221 cggtattgaa attcatccat ggctgcaccg caatgcggac aggggaattc tgttttggct
    17281 tggtggtgga tcaacgagcc cttgcgggaa tcgtcatcga aggccacatc ttcaagtttg
    17341 tccagttcgt taaagtccag ccacatgccg cggcagttgg ggcaggaatc cacttcgatc
    17401 atgcctttgt agtatttttt ggcgagttct gtgttgcatt ttgggcagtt cattttattg
    17461 ttttctccag catggaaaga gaaaaatgtc tattttctca agccgccgtt gggggtgtta
    17521 ttcagtaagg tttgatgtac tttcagccat cgcttcatcc accagatctt caaccgatct
    17581 tccattttcc tgaaaatcga gccagaccag gaattcggca ttgcccttcg gccccagcaa
    17641 aggtgacttg atcaacccgc gcaggttgaa accttcctgt tttgcgaacg tcaacacatc
    17701 cagcaaaacc tgtctgtgaa tctcagggtc gcggatgaca ccatcgccgc gggccacatc
    17761 ttttttaccc gcttcgaatt ggggtttgat gagggcaacg atcccttctt tcttcttttc
    17821 tctttcatcg tcattgaaag aagaaagagg aaagaaagag aaccattttt tgaccaccgg
    17881 cagcaatatc ttcaacgaaa tgaatgaagc atccaccgtc accaaagaca ccgcctcagg
    17941 cagcgactcg acatgccgtg cattggtctc ttccatgaca accacacggg cgtagttgcg
    18001 caatttccag tgcaggatgc ccttgcccac atcaatggcg tagactttgg ccgctccgcg
    18061 ttgcagcagg cagtcggtga atccgccagt ggatgcgccg acatctgcgc agatgaaatc
    18121 tttcacgtca attccgaatc tctccagcgc cgcatccaat ttttcgccgc cacgtgaaac
    18181 aaagcgcgga ccggtatcca ccgtcagcgc tgattttgta tctatcgctg tggcaggttt
    18241 cagggtcact tgatcgttca cgcgcacttg tcccgccatg atgagggcct gagctttcgc
    18301 acgcgattcg gccaatccgc gctccacgag caaaacatcc aatcgaatct t
//
LOCUS       DAOQJC010000080        18265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1422_length_18265_cov_9.478803, whole genome shotgun sequence.
ACCESSION   DAOQJC010000080 DAOQJC010000000
VERSION     DAOQJC010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18265
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1422_length_18265_cov_9.478803"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..120)
                     /locus_tag="PLE39_12500"
     tRNA            complement(34..120)
                     /locus_tag="PLE39_12500"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(84..86),aa:Ser,seq:cga)
     gene            complement(189..1583)
                     /locus_tag="PLE39_12505"
     CDS             complement(189..1583)
                     /locus_tag="PLE39_12505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE69198.1"
                     /translation="MRISFHGAAHTVTGSQHLIEVNGSRLLLDCGLYQGKRADTYERN
                     LNFSHDPRKVDAVILSHAHIDHAGNLPNLVKQGFEGPIYATRATVDLASLMISDSGHI
                     QESDAEFVNKKRAQRGEEPIEPLYTKEDAEKVAGMFSGVNYGEAFEPIPGVVARFYEA
                     GHILGSAGVSLDIEEKGKKTRLWFSGDIGRYKLPLLRDPVLPDRADYMIMESTYGDKS
                     HNDPSKAFIEFRDVVKRTIERGGKVIVPAFAVGRTQELVYHLNEMMTEREIPSVPVFV
                     DSPLAVNATSVFRNHPECYDEETRQFVLEARHPALDFKMLTYVKTVDESKALNERTDP
                     MVIISASGMAETGRIVHHIRNNIENPKNTICIVSWQAPDTMGRRLADREPIIRIFGEA
                     YKRKCDVATIGGLSGHAGQDLLMKYALNVKDSVKQIFLVHGEEKQAMTLKGLLKEQKL
                     NQVHYPDLHESVEL"
     gene            complement(1573..2709)
                     /locus_tag="PLE39_12510"
     CDS             complement(1573..2709)
                     /locus_tag="PLE39_12510"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNE69199.1"
                     /translation="MPNVLPPPVWINSKPLLQKMMDDLVTQKRVAVDTESNSLHAFRE
                     QVCLIQISSSNEDYLIDPLELRDLKLLAPIFSDPKIEKVFHAAEYDLICLRRDFGFSF
                     ANLFDTMHAARVLGYPAVGLDRLLGDKFNIQMDKRHQKANWANRPLTREQIHYARLDT
                     HYLFDLRDALELELREKARWDFAKDDFTRACLPEEIKLRTNGESWERFSGRKDLSLRE
                     LTIMAHLCKWREKEAEKLNRPLYKVIMDDTLITLSKNQAQQKVDLSAAGLSEKQIHLW
                     GEAVLGAIRDGVSAPLVERKQPEVKNDAFLRRLEKLKVWRKNVGQEMGVESDVILPKV
                     YLSALAENPPNTADELAHLLKGTPSRVEKYGPQILKLLGVKNAN"
     gene            complement(2748..3764)
                     /locus_tag="PLE39_12515"
     CDS             complement(2748..3764)
                     /locus_tag="PLE39_12515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNE69200.1"
                     /translation="MTVGKQRLQEFEALRALSIVLLLILHSDVFSLSVFGFELGPISH
                     FVGAFLLGSFFFLAGYFQEVSMQKRGYEPLAYVKSKFLRIFPPYWFALALFIFVMGYS
                     LKRFDLTVYLLNIQFIFSPTFVKQMLTLWYISVVVGYYVLFGLLANRLRSNSGLMIWS
                     AVIFAATYVLHKMTGLLDGRFFEYFFIFLAGVYFARFQQIRERLFSLHFAYKVGVAFL
                     GVLAFRMALTSGVDITHPLYLLAVDFYILGWVWMWLTIFRGGAGAWRIWAPISTASFF
                     AYLFHRPIWYILVGLLAKRMGISTIMFQFIPGSIAVLVICYFLQNGYDTLLRMGRKST
                     RAEI"
     gene            complement(3761..4192)
                     /locus_tag="PLE39_12520"
     CDS             complement(3761..4192)
                     /locus_tag="PLE39_12520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69201.1"
                     /translation="MQIIPATLFDLNSLRKLEKACFEKDAWPLFDLIAVLTFPEVVRL
                     KAVVDGQMAGFVGGDPRPHEGWGWIATIGVDPRFQRRGIGRALLHACEAKLGVPRSRL
                     TVRMSNYAAISLYEQEGYVTTDIWSRYYNDGEDGLVMEKNL"
     gene            complement(4277..5185)
                     /locus_tag="PLE39_12525"
     CDS             complement(4277..5185)
                     /locus_tag="PLE39_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNE69202.1"
                     /translation="MNGVLGSVGALFLCLGLAVVVFLAFNFLRIVPEYQRLVVFRLGR
                     VLDKPKGPGIVLLIPGADRPVKVDLREQVREVPHQTSITKDNAPISIDFLWYYKVLDP
                     AQSVLQVGNFESAAAGIAATTLRAVIGGIPLDDVLSEREHINNMLRTRLDEVTERWGV
                     KVTNVEIREIIPPRDVQDAMNRQMSAERIRRAVVTESTGTREAAVNVAEGEKQSNILR
                     AEGEKQSAILKAEGEREAQLLRAEGFAKALEQIYNSAKTVDQKTMTLQYFETLKAMGM
                     SPSTKYIFPMEFTSMLDNFLGKDTKK"
     gene            complement(5210..5803)
                     /locus_tag="PLE39_12530"
     CDS             complement(5210..5803)
                     /locus_tag="PLE39_12530"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNE69203.1"
                     /translation="MDFLLDPNVAYLIFLTGIVLVFIAVATPGTGLVEIGALFCFILT
                     GYALYNLSVNWWALVILLLSVVPFIQAIRKPKSFLFLALSILLIVTGSVFLFASDDGS
                     LISVNPVIALISSGLVAVFLWFLFRKAIEAISRRPTHDLETLVGQIGEAKSAIYGDGS
                     VYIAGEMWSARSDSEIPAGSHVRVIRRDGFTLVVEKQ"
     gene            5941..7257
                     /locus_tag="PLE39_12535"
     CDS             5941..7257
                     /locus_tag="PLE39_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNE69204.1"
                     /translation="MKFQKICVIGLGYIGLPTATTFAAHGIEVTGVDINQNIIETLKK
                     GEIHIHEPGLRDGFVSAVQSGRFKAATSPEEADAFIIAVPTPFQENEYGSHNGMTYKL
                     ADMRAVISAAESIVPFLRKGNLVVLESTSPPRTTLDLVAPILARSGLEAGRDFHLAYS
                     PERVLPGQILRELIENARVIGGITPESAEAGYELYSTFVKGKIIKTDATTAEMVKLME
                     NTYRDVNIAIANEFSRLANKFGVDVWEAISLANLHPRVKILNPGPGVGGHCISVDPWF
                     LVEAAPELTPLIYHSRRVNDTQPHFVVETVKRALGSLNGKRIAALGLAYKPDVDDLRE
                     SPAAEVVHLLQHEGAQVKAWEPFKPNANMNGIHMSPNLEDAIQDADTILLLVRHTEFK
                     QISPREIAGKTSAKIIVDTVNSWEGEAWKSSGFEFFRIGDSKSPNS"
     gene            7295..8401
                     /gene="wecB"
                     /locus_tag="PLE39_12540"
     CDS             7295..8401
                     /gene="wecB"
                     /locus_tag="PLE39_12540"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNE69205.1"
                     /translation="MKILSVFGTRPEAVKMAPVVKLLEKTAGIESKVCVTAQHRQMLD
                     QVLNLFEIQPDYDLDLMRDNQSLAQLSANIFTHLDPVLDDFKPDWVLAQGDTTTVAIT
                     SLLSYFRRIKFGHVEAGLRTHDKWQPFPEEVNRRIAGVTADMHFAPTEWSRNNLLREG
                     VEESLIKVTGNTVIDALQFVSKQPEPEEIRQLVKEKLASSRLILVTAHRRENFGLPME
                     GICHALKELAQEEGVEIVYPVHLNPNVQEPVNRILKGVDHITLLPPLDYLPLVHLMKH
                     ATIILTDSGGIQEEAPAFGVPTLVLRDVTERPEGVQAGTLKLVGTETSHIVQEAKRLL
                     YDKSAHAQMAKAANPYGDGHAAEKIIHALLENGQ"
     gene            8524..9465
                     /locus_tag="PLE39_12545"
     CDS             8524..9465
                     /locus_tag="PLE39_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69206.1"
                     /translation="MQRRYLTLFLVIFMLSTTWVPAHAQDQGSKYFSETGHNVKGDFL
                     VFYNSNPNALFLYGYPITEEFIDKNNKTVQYFQRARFEYDPNQSAGQRVQLTQLGRQT
                     YISTGKLDVANTLACRTYSETGFAVCFAFLEFFDQYGGLTQFGYPISGFEYHENKIVQ
                     YFEKARLEWQPWKTEGQRVVVSDLGRIYFDALGEDPGLLSPVKPLNNTNQDVISLQVR
                     AFVWKAVTLATDSQLVFVIVQNQNLQPIANATCSTIVNWPDGRSENMSLTTNTNGVGI
                     VPLSFVNQPYGSLIYTDVSCSYGNLSGKTTTSFRIWY"
     gene            complement(9552..10151)
                     /locus_tag="PLE39_12550"
     CDS             complement(9552..10151)
                     /locus_tag="PLE39_12550"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNE69207.1"
                     /translation="MPWSKRIFDFIFALIALIIFSPVLIVTAILVRVFIGTPILFTQQ
                     RPGYKGRPFNIYKFRSMTNRIARDGSLLPDAERLTRFGRILRSLSLDELPELFNILRG
                     EMSFVGPRPLLMEYLPLYSPEQMRRHDAMPGLTGWAQVNGRNALDWPSRFRLDVWYVD
                     HWSFWLDLRIILMTVGKVIAREGVNQAGQSTVEYFKGEQ"
     gene            10219..10896
                     /locus_tag="PLE39_12555"
     CDS             10219..10896
                     /locus_tag="PLE39_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE69208.1"
                     /translation="MNERILIIEDDQAILKLLQRGLAYEGYTVDTATDGRMGLIAARD
                     HTPDLVILDWMLPGMDGLEVCNRLRTGGSIPILMLTAKDTVQDRIQGLDAGADDYMVK
                     PFNLDELLARVRALLRRTQPERIPVLKFADLSLDTGSRQAARGSRIVALTAKEYELLE
                     LFLRHPKQVLTREVIFDRVWGYDFGGESNVLEVYIRYLRQKLEGEGEARLIHTVRGVG
                     YVLRENP"
     gene            10984..12351
                     /locus_tag="PLE39_12560"
     CDS             10984..12351
                     /locus_tag="PLE39_12560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE69209.1"
                     /translation="MSLRLRLTLLYSTFMSGILLIFGAAVYILVNVILLNQVDTMLAG
                     VVRDITQVTKVNPMGELNLISLPPLDMTSNAYVQVWGPDNKIIATSPSIGTLTKPLDP
                     IGLESDMVVYKDSYLSGAHLRVLSVPLKVGDRFIGTLQVGASLSVVDATQGNLLSMMA
                     AIAVVAVLLAGFGSWVVLGRALSPLEAITETVDQINRADDLSRRIPSQERSEDEIGEL
                     ITSFNQTLERLESLFTSQQRFLADVSHELRTPLTVIKGNVDLMRHFKQVDEESLTSID
                     QEAGRLTRLVGGLLMLAQAESGKLTLVMKPVELDLLLTEVFTEMCVLAGSKVHVHLNE
                     IDQVMVNGDRDRLKQVLLNLVANAIQYTPQGGEVFLSMKKIGDQARLIIRDTGPGIPA
                     EDLPHIFDRFYRAEKSRTRSTSSGFGLGLSIAQWIVENHGGQIKVESKEGKGTTFVIW
                     LGIVK"
     gene            complement(12398..13498)
                     /locus_tag="PLE39_12565"
     CDS             complement(12398..13498)
                     /locus_tag="PLE39_12565"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNE69210.1"
                     /translation="MALTIGTAAAKRGGITYGEYALVQHPVGGADSLPVIIAQGEEEG
                     PVFWVTAGIHGIEHAGIQVIHKLITPELVGQMRGTIIAIPALNPAGLRTLNRQAYYHV
                     GDPNRLFPDGRKPKTNDPDQNLPSALEQAYGRLFEEIKNTASFYFDLHCMSNGALSFT
                     FRDRILYRKGDEAARQRAEVVSTKIDEMAQAYGHTVIADFAVEKYIDQKLHRATTAAV
                     SMLAGIPALTAELSSPYTPTPEIVHAGVAGLRNVMRWGGMLGGEMEPILGIKVVDPGF
                     PTHRRSVARVTQSCIVHHLKEPGDLVKVGDPLVEMRDAWGRSLGVLNSEYEGFVISRP
                     TGILYYPGEATMLLAIRDEKPRIGEYPETYFD"
     gene            13550..14383
                     /locus_tag="PLE39_12570"
     CDS             13550..14383
                     /locus_tag="PLE39_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69211.1"
                     /translation="MITALNFPSFDEHFSDLNNFILYLVQQYQTYRLASWEVLAHRCR
                     QFYTPDVMDAIEAKAPGWKKMASYSEGITLEHVTCVFLGMYMLPEFQELSAEQQQMAK
                     WIILFHDIDKAHIRGQRDTMHAFHSALVAARDLPGLGFPVTDKYASLIDAWSEYTRQA
                     FVQPADQNWPKPDNQKLPNILSGIEDMFGEQAPATLIIKTALLHISLLVDRNYQTPAP
                     LTDKEVKRFISPALFPLLRVMMFADNEGWSLFDLEFRDQQKHDSSIEFERIRKFISPT
                     S"
     gene            14380..15684
                     /locus_tag="PLE39_12575"
     CDS             14380..15684
                     /locus_tag="PLE39_12575"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE69212.1"
                     /translation="MTNKSSHDPYAALRLRDFRLLLIGRFITSFGNEMLSFAIGWELW
                     LRTRSAFALGMVGLVQVVPVILLSLPGGHVADQYNRRRIIVITQAVFSLCSAGLALLS
                     FFDGPLVLIYLCLLGFGIARAFNEPASSTLIPETVPSELFASAATWNSSTWQTAAISG
                     PAIAGLVAGFLNSVTIIYVFDAIAALVFLFLVTQIHGKPLPLSEKSDTMASLMEGFRF
                     MRDTKIILAAITLDMFAVLFGGAVALLPIYATDILKVGAQGLGIMRAASSIGALMMAF
                     GLAHLPPFKQAGKTLLWAVAGFGAATIVFGLSRSFWLSVAMLALLGALDNISVVIRST
                     LLLTRTPNEMRGRVSAVNSIFISTSNEMGAFESGLAASLFGPILAVVGGGIGTIIVVL
                     IVARAWPEMRNLKSLADLEEPAPAQFTSPNQGDENAPSSTHP"
     gene            15659..16189
                     /locus_tag="PLE39_12580"
     CDS             15659..16189
                     /locus_tag="PLE39_12580"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE69213.1"
                     /translation="MPLQAPTPDEYASFYSDYIQRAVARGDVLAAMPTQIEELDSALG
                     NLTDKQARFRTGPEEWSIKEIVSHLIDAERVFSYRLLRVSRNDTTPLPGFEQNGYVST
                     SGADDLPLGDLLAEFSFLRRANILAIGHMSTDAVKRRGTVSGATVSANALIHMLVGHV
                     DHHMASLHEKYLPNLH"
     gene            16307..17176
                     /locus_tag="PLE39_12585"
     CDS             16307..17176
                     /locus_tag="PLE39_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69214.1"
                     /translation="MANYQRNLGDGLKTIWNNFIRNRKFNRGAAWGMMIIGALLSFEI
                     FNFSTTQFALRDMLGDLTFAGFRWATILAIAFCGIDFAGIARIFTPEQGRDEPAEVYY
                     LFGAWLLAAGFNATLTWWGVSVAVAENGNVHAAGALSAETLTKAVPIFVAVMVWLVRL
                     LIIGTFSLAGDRLFTTADQPSAQHRPQYQSQPQTQRAYNNPQPQQRPAYQNSQPQRPS
                     YNQPVNQPILRPASQIARPANSPSTTNFRPAPKPTVNSTQTSFIPPEPTYHPISYEAR
                     TNSVESSTRHYDA"
     gene            17295..17984
                     /locus_tag="PLE39_12590"
     CDS             17295..17984
                     /locus_tag="PLE39_12590"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNE69215.1"
                     /translation="MPRARTSQIDRQTAAPQQEQGSGCFSGFMLVPLSVIFISTLLAA
                     LAMKTTPIPSSRVDLPPSTSTGIALIFTPEIQYWAEDIAQWAAASQLDPNLVATIMQI
                     ESCGDPRAISSAGAMGLFQVMPFHFAASDSPYDPDTNAARGLAYLVRSLQAGGGDARL
                     ALAGYNGGIGILSRAEWTWPAQTKRYIQYGVPIYQDARNGLPHSEAVTEWHETYGASL
                     CRQAHNRLGLP"
BASE COUNT         4557 a         4954 c         4416 g         4338 t
ORIGIN      
        1 aaaaaagccc aaccgatttg gctggacttt tagcggagag ggagggattc gaaccctcgg
       61 tggaccggag cccacaacag ttttcgagac tgtcccattc aaccactctg gcacctcccc
      121 atttctcgaa gcgggcgaaa ttataaccga aaaaaatacg cccaatccgt tcaatatatg
      181 gagtgtgttt acaactccac actctcatgt aaatcggggt agtgaacctg attcagcttc
      241 tgttccttca aaagcccctt caaggtcatc gcctgcttct cttcgccatg gaccaaaaat
      301 atctgcttga cgctgtcttt cacattcaaa gcgtacttca tcaacagatc ctgccctgca
      361 tggcctgaga ggccgccgat ggtggcaaca tcacatttac gtttataggc ttcaccaaaa
      421 atgcggatga tcggttcacg gtctgcaagc ctgcggccca tcgtatccgg tgcctgccat
      481 gaaacaatgc agatcgtatt ctttgggttt tcgatattat tgcgaatatg atggacgata
      541 cgccctgttt ccgccatgcc cgaagcggaa atgatcacca tcggatcggt acgttcattc
      601 aacgccttgg attcatccac agtttttaca taagtcagca tcttgaaatc cagggctggg
      661 tgacgtgcct ccaacacaaa ctgacgggtt tcttcatcgt aacattcagg gtgattgcga
      721 aaaaccgaag tggcattcac agccagcgga ctatccacaa acaccggcac actgggaatc
      781 tcgcgttcgg tcatcatctc attcaagtga taaaccaatt cctgcgtacg ccccactgca
      841 aaagcgggca cgatcacctt gccgccgcgc tcgatcgtcc gcttgacgac atcgcgaaat
      901 tctataaaag ccttcgacgg gtcgttgtga cttttatcgc cataggtcga ttccatgatc
      961 atgtaatcgg cacggtctgg caaaacaggg tcgcgcaata atggcagttt atatctgccg
     1021 atatcaccag aaaaccacaa gcgggtcttc tttccctttt cttcaatatc cagcgaaacg
     1081 cccgccgaac cgaggatatg ccccgcttca taaaaacggg cgaccacgcc agggatcggt
     1141 tcaaacgcct ccccgtaatt cacaccgctg aacatgcctg caactttctc agcatcttct
     1201 tttgtataaa gcggctcaat gggttcctca ccacgctggg cccgcttttt gttcacgaat
     1261 tccgcatctg attcttgaat gtgtccagaa tcggagatca tcaggctggc cagatccact
     1321 gtagcgcggg tggcatagat cggcccttcg aagccctgct tcacaaggtt gggcaggttc
     1381 ccggcatggt caatgtgggc atgagacagg atcaccgcat ccaccttgcg cggatcgtgg
     1441 ctgaaattca aatttcgttc gtaggtatcg gccctttttc cctggtacag gccacagtcc
     1501 agaagaagac gcgaaccgtt gacttcgatc aaatgctggc tgccggtcac agtatgggcg
     1561 gcgccgtgga aactaattcg catttttgac tcctaataac tttaatatct gcgggccata
     1621 tttttccaca cgtgaaggag tacccttcaa caagtgagcc aattcatcag cagtgttagg
     1681 cgggttctca gccagcgctg ataaataaac ctttggcaga atgacatctg actcgacgcc
     1741 catttcctgc ccgacattct ttctccacac cttcagcttt tccaaccgac ggagaaacgc
     1801 atcattctta acctcaggct gtttccgttc aaccagcgga gccgacaccc catcacggat
     1861 ggcccctaaa accgcctccc cccacaagtg gatctgtttt tcactcaagc ccgccgcaga
     1921 aagatccact ttttgttggg cctgattttt cgacagggtg atgagggtgt catccatgat
     1981 caccttgtaa agcggacgat ttaatttttc agcttccttt tcacgccatt tgcacaaatg
     2041 cgccatgatg gtcaactcgc gcagcgacag atccttgcgc cctgaaaacc gctcccacga
     2101 ctctccattt gttcttaact tgatctcttc cggcaggcag gcgcgggtga aatcatcttt
     2161 cgcaaaatcc caacgggctt tttcacgcag ttccagctca agggcatcgc gcaggtcaaa
     2221 aagataatgt gtatccagcc gcgcataatg gatctgttct ctggtcagcg gacgattggc
     2281 ccaattggct ttttgatggc gcttgtccat ttggatattg aatttatcgc caagtaaacg
     2341 gtcaagtcca acggcggggt atcccagcac acgggcggca tgcatcgtat cgaaaagatt
     2401 tgcaaaggaa aaaccaaaat cacgccgcag acagatcagg tcatattcgg cggcatggaa
     2461 tactttttca atttttgggt cggagaagat cggcgccagc agtttcaagt ccctcaactc
     2521 aagcgggtcg atcaaataat cttcattgga tgaagaaatc tgtatcaggc aaacctgctc
     2581 gcgaaaggcg tgcaggctgt tcgattctgt atccaccgcc acacgtttct gtgtgacgag
     2641 gtcatccatc attttttgta ataggggttt tgaatttatc cagacgggcg gtggcaacac
     2701 attgggcata tcggccgatt ataaaccttc tacaggcagg cgaggaatca aatctcggcc
     2761 cgcgttgatt ttcggcccat ccgcaaaagg gtatcgtatc cgttttgcaa aaaatagcag
     2821 atgacgagga cagcgatcga gcctggaata aactgaaaca tgatggtgga tattcccatc
     2881 cgttttgcca gtaaaccaac caaaatgtac caaatggggc gatggaatag atatgcaaaa
     2941 aacgaagctg tggaaatcgg cgcccaaatt cgccatgccc ccgccccacc ccggaagatc
     3001 gtcagccaca tccataccca gccaaggatg taaaaatcca cagccagcag gtagagcgga
     3061 tgcgtgatat ccacacccga ggtcaatgcc atacgaaacg ccagcacgcc caaaaacgca
     3121 acgccaactt tgtaggcgaa gtgcaggcta aaaagccgct cacgaatctg ttgaaagcgt
     3181 gcaaaataaa cgcctgccaa aaagatgaaa aagtattcga aaaacctgcc atccagcagg
     3241 cctgtcattt tatgcaacac gtaggttgca gcaaagatca ccgccgacca gatcatcagt
     3301 ccactattgg atcggagacg atttgctaga agaccgaata aaacgtaata tcccaccact
     3361 acgctgatat accacaaggt caacatttgc tttacgaagg tcggcgagaa gatgaattga
     3421 atgttcagca ggtacaccgt caggtcgaag cgtttcagcg aataccccat cacaaaaata
     3481 aacagtgcca gcgcaaacca atagggtgga aaaatgcgca ggaatttgga tttcacatat
     3541 gccagcggtt catatccgcg tttttgcatg gagacttcct gaaaatatcc cgccagaaag
     3601 aaaaaggatc ccagtaaaaa agcgccgaca aaatgcgaaa tcggtcccaa ttcaaaacca
     3661 aaaacgctca aagaaaacac gtcactatga aggatcaaca acaggacaat ggaaagcgca
     3721 cgcaatgctt cgaattcctg taaacgctgt tttccaaccg tcataaattc ttctccataa
     3781 ccaacccatc ctcgccgtcg ttgtagtatc tgctccaaat atcggtggtg acgtagcctt
     3841 cctgttcgta aagggaaatg gccgcataat tggacatgcg tactgtcaaa cgggaacgcg
     3901 ggacacccaa tttagcctcg caggcatgca gcagggcccg cccaatgccg cgccgctgaa
     3961 agcgtggatc caccccaatg gtggcgatcc agccccaccc ttcgtgtgga cgcgggtctc
     4021 ctcccacaaa tcctgccatt tggccatcca ccactgcttt gagtcggacg acttcaggaa
     4081 aggtcaacac cgcgatcaaa tcgaagagcg gccatgcatc tttttcaaag caggcttttt
     4141 ccagtttacg aagtgaattc aggtcgaaaa gtgtggctgg gatgatttgc atgacccgat
     4201 gattttatca ataaaaaagc aggaagcgga atatccgcct cctgcttttt acgtcattta
     4261 ttttttgaaa gctgttctac ttcttggtat cttttccgag gaagttatcc aacatgctgg
     4321 tgaattccat cgggaagatg tatttggtgg aaggactcat gcccatggct ttgagggttt
     4381 caaagtattg cagggtcatg gtcttctggt ccacagtctt ggctgagttg tagatctgct
     4441 caagggcttt tgcgaaacct tcggcgcgga gcagctgggc ttcgcgttca ccttccgcct
     4501 tgagaatggc cgattgcttt tcaccttcag cacggagaat gttggactgt ttttcacctt
     4561 ccgccacatt aacggcggct tcgcgtgtac ccgtggattc ggtcaccacg gcgcggcgga
     4621 tacgttcggc agacatctga cggttcatcg cgtcttgaac atcgcgcgga ggaatgatct
     4681 cgcggatctc tacgttggtc actttcacgc cccagcgctc agtgacttca tcaaggcggg
     4741 tgcggagcat gttgttgatg tgttcacgtt cggagagcac atcatcaaga gggataccac
     4801 cgatgaccgc gcgcaaagtg gtggccgcaa tacctgccgc cgccgattca aagttaccca
     4861 cctgcaaaac cgactgtgcg gggtcaagca ccttgtaata ccacaggaag tcgatggaga
     4921 tcggggcgtt atctttggta atggaggtct gatgaggaac ctcgcgcacc tgttcgcgca
     4981 aatccacttt tacggggcgg tcggcaccag ggatgagcag gacgatacca gggcctttgg
     5041 gcttatccaa tacacggccg aggcggaaca caaccaatcg ttgatactct ggcacaatgc
     5101 gcaggaaatt gaacgccaaa aatacaacca cagccaggcc cagacataaa aataaagcac
     5161 ctaccgaacc aagaacacca ttcatatgtt ttctccttgt atgaaatgat tattgttttt
     5221 ccacaaccag ggtaaagcca tcacgccgaa tcacgcggac atgactccca gctggaattt
     5281 cagaatcgct gcgtgcagac cacatctccc cagcaatata tacgctccca tcgccataaa
     5341 tggctgactt cgcctcaccg atctgtccca ccaacgtctc aaggtcatgg gtgggtctgc
     5401 gggagatcgc ctcaatggct ttgcgaaata aaaaccacag aaaaacagcc accaaaccgg
     5461 aagagatcaa ggcaatgacc gggttcactg aaatgaggct gccatcatca ctggcaaaaa
     5521 ggaacaccga cccagtcact atcagcaaaa tggacagcgc caaaaaaaga aaactttttg
     5581 gttttcgtat ggcttgaatg aacggcacca cgctcagcaa caaaataacc agagcccacc
     5641 aattgaccga caggttatac aacgcatacc ctgtcaatat aaagcagaac aaggcgccga
     5701 tctccaccaa tcccgtccct ggggtggcta cggctataaa gaccaacaca atgccagtga
     5761 gaaatattag ataggcaacg ttcgggtcaa gtaaaaagtc cattgttcct ccatttgatt
     5821 atacaacaag aagcaaggtt tcgattcggt atacggaaaa tgtcgtgcga aggttgcgtc
     5881 agactttgcc gcaagcggtt gtataataca cttctgcaaa aacaccatcc aggagcccta
     5941 gtgaaattcc aaaaaatctg tgtcattggg ctgggctaca tcggcctccc cacagcaacc
     6001 acattcgccg cacacgggat cgaagtgaca ggcgtagaca tcaaccaaaa tatcattgaa
     6061 accctcaaaa agggcgagat tcatattcat gagcctggcc tgcgtgatgg tttcgtcagt
     6121 gcagttcaat cgggcaggtt caaagccgcc accagccccg aagaagcaga tgccttcatc
     6181 atagccgttc ccaccccatt tcaggaaaac gaatacggct cacacaatgg gatgacctac
     6241 aaactggccg atatgcgtgc tgtgatttca gccgccgaat ccatcgtgcc gttcttgagg
     6301 aaggggaatt tggtggtgct cgaatcgacc tcaccgccac gcaccacact cgaccttgta
     6361 gcccccatcc tcgctcgttc tggacttgag gctgggcgcg acttccattt ggcatattct
     6421 cccgaaaggg ttttgcccgg gcagatcctg cgcgaactga tcgaaaatgc acgcgttatc
     6481 ggcggcataa cccctgagtc cgccgaggca ggctacgagt tgtattccac tttcgttaaa
     6541 ggcaagatca tcaaaaccga cgccaccacc gcggaaatgg tcaaattgat ggaaaacacc
     6601 taccgcgatg tgaacattgc catcgccaac gaattctcgc gccttgccaa taaattcggc
     6661 gtggatgttt gggaggcgat ctcactggcc aacctgcacc cgcgtgtgaa aatccttaat
     6721 ccagggcctg gtgtcggtgg tcactgcatc agcgttgatc cctggtttct ggtggaagcc
     6781 gcacccgaat tgaccccgct catttaccac tcaaggcggg ttaatgacac tcaacctcat
     6841 ttcgtcgtgg agaccgtcaa acgcgcattg ggcagcctca acggcaaacg catcgccgcc
     6901 cttggcctgg cctacaaacc cgacgtggat gaccttcgtg aaagccctgc ggctgaggtg
     6961 gtccatctcc ttcaacatga aggcgcacag gtcaaggcct gggagccttt caagccgaac
     7021 gcaaacatga acggcataca catgtcgcca aatcttgaag atgccattca ggatgccgac
     7081 accattttgc tgctggtcag gcacaccgaa ttcaaacaga tcagcccccg cgagatcgcc
     7141 ggcaagacca gtgcaaaaat catcgtagat actgtcaaca gctgggaagg cgaagcctgg
     7201 aagagcagcg gcttcgaatt cttccgcatc ggcgacagca aatcaccgaa ctcgtaaacc
     7261 gatcacccat ccttgtgttg aggcacttgt aacgttgaaa atcctctctg tttttggaac
     7321 ccgccctgaa gccgtaaaaa tggctccagt tgtaaaactg ctggaaaaaa ccgcaggcat
     7381 cgagtcaaag gtctgtgtca cagcccagca cagacaaatg ctcgatcagg tccttaacct
     7441 gttcgagatc cagccagatt atgacctcga cttgatgcgc gataaccagt cgcttgccca
     7501 gttgagcgca aatattttca cccacctcga tcctgttctg gatgatttca aacccgattg
     7561 ggtgctggcc caaggtgata cgacaacggt tgccatcacc tccctgcttt cgtacttccg
     7621 ccgcatcaag tttgggcatg tggaggcagg cttgcgcaca cacgacaaat ggcagccctt
     7681 ccctgaagag gtcaatcgcc gcattgccgg tgtcactgca gatatgcact tcgcccccac
     7741 cgaatggtcg cgtaacaatc tgttgcgcga gggtgtggaa gaaagcctga tcaaggtgac
     7801 gggcaacaca gtgatcgatg cactgcaatt cgtatccaaa caacctgagc ctgaagagat
     7861 cagacaactg gtaaaggaaa aactggcttc ttcacggctt attctcgtca ccgcgcatcg
     7921 cagggagaat tttggcctcc ccatggaagg tatctgtcat gctttgaaag agcttgcaca
     7981 ggaagaaggg gtcgagatcg tgtaccctgt gcatctaaac cccaatgtgc aggaacctgt
     8041 gaacaggatt ctcaaaggcg tggatcacat caccctcctt ccgcctctgg actacctgcc
     8101 acttgtccat ttgatgaaac atgccaccat catcctgaca gattcaggcg ggattcaaga
     8161 agaagcccct gcattcggcg tgcccaccct tgttttacgc gacgtaactg aacgtccgga
     8221 gggagtccaa gcggggacgt taaagctggt tggaacagag accagccaca tcgttcagga
     8281 agcgaagcgg ctgttgtatg acaaatctgc gcatgcacaa atggcgaagg cagccaatcc
     8341 atatggtgat ggtcatgcgg ctgagaaaat catccatgcc ttgctggaaa atggtcagta
     8401 aaactaccag gttcccgcct tgcatttttt tttggaagat agccacctgt aatgtcaaaa
     8461 aagaagtttg taatgttaca cgaaaaaatt ctgagtataa tggaaacaga gatgacataa
     8521 gccatgcaac gcagatacct caccctgttt ctcgtcatat ttatgctcag tacaacctgg
     8581 gtacctgctc acgcgcaaga ccaaggctca aagtatttta gtgagactgg tcataatgtc
     8641 aaaggggatt tcctggtctt ttacaacagc aaccccaatg ccctcttcct gtatggttac
     8701 cccatcacag aagagtttat cgataaaaac aacaagacgg ttcaatattt ccaacgcgcc
     8761 cgttttgaat atgacccgaa ccaatctgca gggcagcgcg ttcaactcac ccaacttggc
     8821 cgccagacct atatctccac aggcaagtta gatgttgcga atacccttgc ctgccgcacc
     8881 tacagtgaaa ctggttttgc cgtttgtttt gcctttcttg aattctttga ccaatatggc
     8941 ggtttgaccc aattcggtta ccccatttca ggttttgaat atcacgagaa caagatcgtc
     9001 cagtactttg aaaaggcccg ccttgaatgg cagccttgga agacggaagg tcagcgtgtg
     9061 gttgtttcag acttgggcag aatttatttc gatgcgcttg gtgaagaccc tgggttgctc
     9121 tcccctgtca aaccgctgaa caacaccaat caggatgtga tcagcctgca agtccgtgct
     9181 tttgtttgga aggcggtcac cctcgccacc gactcacagc ttgtattcgt catcgtgcag
     9241 aaccaaaacc tgcaaccgat cgccaatgcc acctgctcaa ccattgtcaa ctggccggat
     9301 gggcgctcag aaaacatgtc gctgaccacc aacaccaacg gagtgggcat cgtgccgctc
     9361 tctttcgtca atcaaccata tggcagcctg atctacacag acgtttcctg ctcttatgga
     9421 aatctgagcg gcaagaccac cacatcgttc agaatttggt actaaattcc atctaaaaac
     9481 aacacagaac cgccctcgaa tgatcaattt cggagggcgg tttgatttta agatcaaata
     9541 tataacagtt tctattgttc ccctttgaaa tactccaccg tcgattggcc cgcctgattt
     9601 accccctctc tggcgatgac tttgccaacg gtcataagaa tgatgcggag atccagccag
     9661 aacgaccagt gatctacata ccatacatcc aggcggaagc gggatggcca gtcaagcgcg
     9721 tttcgcccgt tgacttgagc ccagcccgtc agtccgggca tggcatcatg tctgcgcatt
     9781 tgttcgggtg aatacaacgg aaggtattcc atcaacagcg gacgcggacc gacaaaactc
     9841 atctcacctc gcaaaatgtt gaagagttcg ggcaattcgt cgagcgagag ggaacggaga
     9901 atcctgccaa agcgtgtgag tcgctcggcg tccgggagca ggcttccgtc acgtgcgatg
     9961 cggtttgtca tcgagcggaa tttatagata ttgaatggac gtcccttgta cccgggtctc
    10021 tgctgggtaa acagaatcgg tgttccaata aaaacacgga cgagaatcgc agtaacgatc
    10081 aggacgggcg aaaaaatgat cagcgcgatc aacgcaaaga taaaatcgaa aatccgtttg
    10141 ctccatggca tgttcgtatt ataaccagac tttggaagtg cccaaagttt atggtatatt
    10201 ttgcagagga gtatagccat gaatgagcga atactaatca ttgaagacga tcaggcaatt
    10261 ctaaaattac tgcaacgggg tctggcatat gaaggctaca cggtggatac cgccaccgac
    10321 gggcgcatgg gtttgattgc cgcgcgcgac catacccccg atcttgtgat attggattgg
    10381 atgctgcctg gcatggatgg tcttgaagtt tgtaatcgcc tgcggactgg gggttcgatt
    10441 ccgatcttga tgctgactgc aaaggatact gtgcaggacc gcattcaagg cctggatgca
    10501 ggtgcggacg attacatggt aaagccgttc aatctggatg aattgctggc gcgtgtgcgt
    10561 gccctgttac gccgtacaca acctgagcgc atccctgttt tgaaatttgc cgatctgtca
    10621 ctcgatactg gttcacggca ggctgcacgc ggaagccgca ttgtggcatt gaccgcgaaa
    10681 gagtatgaac tgcttgagtt gttcctgcgc caccccaaac aggtattgac tcgcgaggtc
    10741 atctttgacc gtgtgtgggg ttatgatttc ggcggtgaga gcaatgtgct tgaagtgtac
    10801 attcgctatc tgcgccaaaa actggagggc gaaggggagg cccgtcttat ccataccgtg
    10861 cgcggcgtgg ggtatgtgtt gagggaaaat ccctagttga aaagcggcag cattgacctg
    10921 ccgcttcttt tttgtatgac gatttctcag gttaccctaa actcacggga ttaaactttt
    10981 tacatgtcat tacgcctgcg cctcaccctt ttatattcca cgtttatgag cggaatcctg
    11041 ctcatttttg gcgcagcggt ctatatcctt gtcaatgtga ttctgctaaa ccaggtggat
    11101 accatgctgg cgggtgttgt gcgtgatatt acgcaagtga ccaaggtcaa cccgatgggc
    11161 gaattaaact tgatcagcct gccacccctg gatatgacct cgaatgcata tgtgcaagtc
    11221 tggggacctg acaacaagat cattgcaacc tccccgagta tcggcacatt gaccaaaccc
    11281 cttgacccca tcggtcttga atcggacatg gtcgtttata aagattccta tttgagcgga
    11341 gcccatttgc gggttttgag tgtgccgctt aaagttggtg atcgcttcat tggcacgctg
    11401 caagtcggtg cgagtttgag cgtggtggat gccacgcaag gcaacctgct ttcgatgatg
    11461 gcagccattg cagtggttgc agttttgctg gctgggtttg gttcatgggt ggttttggga
    11521 cgcgcccttt ctccgctgga agccatcaca gaaaccgtcg atcagatcaa tcgtgcggac
    11581 gacctttcgc gacgcattcc ttcacaggaa cgttctgaag acgagatcgg cgagttgatc
    11641 acttcgttta atcaaacgct tgaacgcctt gaatcgcttt tcacctccca gcaaagattc
    11701 cttgccgatg tcagccatga gctgcgtaca ccactcacgg tcattaaagg caacgtagac
    11761 ctgatgcgtc acttcaaaca ggtggatgaa gaatcgctca ccagcataga ccaggaagcg
    11821 ggtcggctta cccgccttgt cggcggcctg ctcatgctgg cacaggcgga atcaggcaag
    11881 ctgaccctcg tcatgaaacc cgttgaactt gatcttttac tgacagaagt gttcaccgaa
    11941 atgtgtgttt tggcgggctc caaggtgcat gtccacctca acgagattga ccaagtcatg
    12001 gtcaatggag atcgagatcg tttaaagcag gtgttgctaa atctggttgc gaacgcgatc
    12061 caatatacgc cgcagggcgg agaggttttc ctcagcatga agaagatcgg agaccaggcc
    12121 cgcctcatca tacgcgacac aggcccgggc atccctgcag aggacctgcc ccatatcttt
    12181 gatcgtttct accgtgctga aaaatcgcgc acccgctcca catccagtgg gtttgggctg
    12241 ggtctttcca ttgcgcaatg gatcgtggaa aatcacggcg ggcagatcaa ggttgaatcg
    12301 aaggaaggca aaggcacgac ctttgtgatc tggctgggaa tcgtgaaata gttttccaca
    12361 aaaaatgcat ggggatgatc gttttttcaa gcagccttta atcgaaataa gtttcagggt
    12421 actcgccgat cctcggtttt tcatcacgga tcgccaacag catggttgcc tcaccggggt
    12481 aatataaaat cccagtcggg cgcgaaataa caaagccttc gtattctgaa ttcaacacac
    12541 cgagagatcg tccccaggca tcacgcatct ccaccaacgg atcaccaacc tttacgaggt
    12601 cacctggctc cttaagatgg tggacaatgc aggattgtgt cacccgtgcc acgcttcggc
    12661 gatgcgttgg aaagcccggg tcaacgacct tgatccccag gatcggctcc atttcaccgc
    12721 ccaacatgcc gccccagcgc atcacatttc gcagacccgc cacacccgca tggacaattt
    12781 caggggtggg cgtatatggg ctgctcaatt ccgccgtcag cgcagggatg cccgccagca
    12841 tggataccgc cgcagtagtg gcacggtgca atttctggtc gatatatttc tccacggcaa
    12901 aatcggcaat cacggtatga ccataggcct gtgccatctc atctattttt gtactcacaa
    12961 cttccgcacg ttggcgggcg gcctcatctc ccttgcgata taaaatacga tcacggaagg
    13021 tgaaagacaa agcgccattg ctcatacagt gcaggtcaaa atagaacgaa gccgtatttt
    13081 ttatttcctc aaataaacgc ccgtacgcct gttcaagcgc ggaaggcagg ttttggtctg
    13141 ggtcatttgt tttcggcttg cgcccatcag gaaagagacg gtttggatca cccacatggt
    13201 aataagcctg acggttcaag gttcgcaaac cagcggggtt caaggcaggg atcgcgatta
    13261 tcgttccgcg catctgaccg accaactctg gtgtgatcaa cttgtgaatc acctgtatgc
    13321 ccgcatgctc aatgccgtgg atgccagcag taacccaaaa aacaggtcct tcttcctctc
    13381 cttgcgcaat gatgacaggc agagaatctg cgccgccaac ggggtgttgt accaaagcat
    13441 attcgccata agtgattccg ccgcgtttgg ccgcagctgt gccgatggta agtgccatat
    13501 aaaaaatcct tttgggtatg tttttgaata tcgtgagtat aatctgatta tgattaccgc
    13561 ccttaatttt ccaagtttcg atgagcattt ttcagactta aacaacttca ttttatattt
    13621 ggtacagcag tatcagacct atcggctggc ttcatgggaa gtccttgcac atcgttgccg
    13681 tcagttctac accccagatg tcatggatgc gattgaggca aaggcaccag ggtggaaaaa
    13741 aatggcttcc tactctgaag gcatcaccct tgaacatgtg acttgcgtat tcctcggcat
    13801 gtacatgctg cctgaatttc aagaattatc cgcagaacag cagcaaatgg caaaatggat
    13861 cattttgttc cacgatatag acaaggcgca tattcgcgga caaagagaca ccatgcatgc
    13921 ctttcattcg gcgctcgttg cagccagaga cctgcccggg ctgggctttc cagtgaccga
    13981 taaatatgcc agcttgattg atgcatggag cgaatacacc cgccaggctt ttgtacaacc
    14041 cgcggaccag aattggccta aacccgataa tcaaaaactc cccaatatcc tttcaggtat
    14101 tgaagatatg ttcggagagc aggctcccgc cacattgatc atcaaaaccg ccctgctgca
    14161 tatttccctg cttgttgaca ggaactacca gacccctgcc cctctgaccg ataaggaagt
    14221 gaagcgtttt atctccccag ctttgtttcc acttttgcgg gttatgatgt tcgcggataa
    14281 cgaaggctgg tctctttttg acctggaatt ccgcgaccag caaaagcacg actcgtccat
    14341 cgagttcgag cggattcgaa aatttatttc tcccacctca tgacaaacaa atcatcccac
    14401 gatccatacg ccgcacttcg cctgcgggat tttcgccttt tgcttatcgg ccgcttcatc
    14461 acctcttttg gaaatgaaat gctttcattt gccattggct gggaactgtg gctgcgcacc
    14521 cgcagtgcat ttgcgctggg aatggtgggg ctcgtgcagg tggtgcctgt cattttatta
    14581 tccctgccgg gcgggcatgt ggctgatcaa tacaatcgca ggcgcatcat tgtcattacg
    14641 caggctgtct tttccctttg ctcggctggg ttggccctgc tttctttttt tgatggcccg
    14701 ctggttctga tctacctgtg cctgctgggg tttggtattg cgcgcgcctt caacgaaccc
    14761 gcctcctcca cactcattcc cgaaacggtt ccttctgaac tgtttgccag tgcggccaca
    14821 tggaacagca gcacctggca gacagcggct atttcaggcc ctgccatcgc tggcctcgtt
    14881 gcgggctttt taaacagcgt gaccattatt tatgtttttg atgcaattgc cgcacttgtg
    14941 ttcctgtttt tagtcaccca gattcacggc aaaccgcttc cgctttcaga gaaatctgac
    15001 acaatggcat cgctcatgga aggtttccgt tttatgcgcg acaccaagat cattctggca
    15061 gccatcacat tggatatgtt tgccgtgttg tttggcgggg cagtggcttt gcttcccatt
    15121 tacgcaacag acattctcaa agtcggcgca cagggattgg gcatcatgcg tgcggcttca
    15181 tccattggag cgttgatgat ggctttcggg ctggcgcatt tgccgccgtt caaacaggcg
    15241 gggaaaactc tcctttgggc agtggcaggc ttcggcgcgg ccacaatcgt gtttggtcta
    15301 tcccgttctt tttggttatc ggtcgccatg ctggctttgc tgggagcatt ggataatatc
    15361 agtgtggtca tccgcagcac actgctcctc acacgcactc ccaacgaaat gcgcggacga
    15421 gtctctgccg tcaacagtat ttttatcagc acatcgaacg aaatgggcgc atttgaatca
    15481 gggctggccg cctctttatt tgggccgatc ctcgctgtgg ttgggggtgg aatcggcacg
    15541 atcattgtcg tcttaatcgt tgcaagggcc tggcctgaaa tgcgcaattt gaaaagtctt
    15601 gcagaccttg aagagccagc gcctgcccaa ttcacttcac caaaccaagg agacgaaaat
    15661 gccccttcaa gcacccaccc ctgatgaata tgcctcgttt tattctgatt acatccaacg
    15721 cgccgtggct cgtggcgatg ttctggccgc catgcccaca caaatagagg aacttgactc
    15781 tgctcttgga aacctgaccg acaaacaagc ccgcttccgc actggacctg aggaatggtc
    15841 catcaaagag atcgtcagcc atctgataga tgccgaacgg gttttttcct atcggctgct
    15901 gcgcgtttcc cgcaacgaca ctactcccct gccaggtttc gagcaaaatg gctatgtgag
    15961 cacctcaggt gccgatgacc ttccattggg agatctgctt gccgagtttt cgtttttgcg
    16021 acgtgccaac atccttgcca tcggtcacat gagcacagac gctgtgaagc gccgcggcac
    16081 tgtcagcggg gccactgtga gcgcaaatgc cctcatccat atgctggtgg ggcacgtaga
    16141 tcaccacatg gccagcctgc acgagaaata cctgccaaac cttcattgac cgaaggaaaa
    16201 ggggtttttt cggggtttcc gtcacctggt gagcgattaa caattccctt gacaatacag
    16261 aacgtttgtt ctaaaattcg acctacctaa cccattggag gtcgaaatgg caaactatca
    16321 acgtaatctc ggcgatggct tgaaaaccat ctggaacaat ttcatccgca accgcaaatt
    16381 taatcgtggc gctgcctggg gcatgatgat catcggcgca ttgctgtcgt ttgaaatctt
    16441 caacttcagc acgacccaat ttgccctgcg cgacatgctc ggtgacctta cttttgcggg
    16501 gttccgttgg gccaccattc tggcgattgc cttctgcgga attgacttcg caggcattgc
    16561 ccgcatcttc acccccgagc agggacgcga cgaacctgcc gaagtatatt atctctttgg
    16621 cgcctggctt ctggcggcag gtttcaatgc cacactgacc tggtggggcg tttctgtagc
    16681 agtggctgaa aatgggaacg tacatgccgc tggtgcgctc agcgccgaaa ccctgaccaa
    16741 agctgtgccc atctttgtcg ctgtgatggt ctggctggtg cgtcttttga tcatcggcac
    16801 tttctctctt gcgggagatc gtctcttcac tacggccgac cagccgtcag cgcagcacag
    16861 accgcagtat caatctcaac cgcagacaca gcgcgcttac aacaatcccc aaccccagca
    16921 gcgccccgca tatcaaaatt cacagccgca gcgcccatct tataaccaac ctgtgaatca
    16981 gcccattttg cggcctgcct cgcaaattgc ccgccctgcg aacagcccaa gcacaaccaa
    17041 tttccgccct gcaccgaaac cgacggtcaa ttccacgcag acctctttca ttccgcctga
    17101 gccgacatac cacccgattt cctacgaagc ccgcaccaac agtgttgaaa gctccacccg
    17161 ccactacgac gcctaatcgt tgatgtcttc aaccgccatg gagtttgttg caacgcttcc
    17221 gccaaaataa tacaactgta taaataaatt ttcgagcacg ggtccttgag acccagaaaa
    17281 gagaatataa cgttatgccc cgcgctcgca catcgcaaat tgatcgccaa acagccgcgc
    17341 cccagcagga acaagggagc ggctgttttt ctggttttat gcttgtcccg ctttcggtca
    17401 tctttatcag tacgctgctt gcggcacttg ccatgaaaac gaccccgatc ccctcttcac
    17461 gtgtggattt gccgccttcc acttcgacag gcattgctct catcttcacg cctgagattc
    17521 aatattgggc agaagatatc gcccaatggg cggccgcttc gcaactggac ccgaatctgg
    17581 ttgccaccat catgcagatc gaatcctgtg gagatccacg tgccatttcc agtgctggag
    17641 ccatgggttt gtttcaagtg atgccgttcc atttcgccgc atctgactcg ccatacgacc
    17701 cagatacaaa cgccgcccgc ggactggcgt atctggtccg ctcgcttcag gcaggcggag
    17761 gagatgcccg ccttgcactg gctggttaca atggcggcat cggcatcctc agccgcgccg
    17821 aatggacatg gcccgcccaa acgaaaagat acattcaata cggcgtcccc atctatcagg
    17881 atgcacggaa cggcctaccc catagtgagg ctgttactga gtggcacgaa acatatggcg
    17941 ccagtctgtg ccgtcaggcg cataacaggc ttggactgcc ataacttatt cttcaaaaca
    18001 tgggaataag ggatgataga atcaaccata ttgtgaacct tcttattgaa aagcaagaag
    18061 gcatatccag tttaatccca ggctattgaa aatctcgtga aaatctcaaa cacactctat
    18121 gtgacggatc ccaaagactg gcggacttgg ctcaagcagc attacaaaac cgaagccgaa
    18181 gtctggctgg tgtaccccaa gaaagcgaca ggcaagccgc gcatcgaata caacgacgcc
    18241 gtggagcaag ccctgtgctt cggct
//
LOCUS       DAOQJC010000081        18261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1423_length_18261_cov_10.256454, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000081 DAOQJC010000000
VERSION     DAOQJC010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18261
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1423_length_18261_cov_10.256454"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..143
                     /locus_tag="PLE39_12595"
     rRNA            <1..143
                     /locus_tag="PLE39_12595"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            223..339
                     /gene="rrf"
                     /locus_tag="PLE39_12600"
     rRNA            223..339
                     /gene="rrf"
                     /locus_tag="PLE39_12600"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            579..776
                     /gene="rpsU"
                     /locus_tag="PLE39_12605"
     CDS             579..776
                     /gene="rpsU"
                     /locus_tag="PLE39_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNE69216.1"
                     /translation="MATVNLRNGESQDSLLKRFRKKIVKSGILSTIRNKRWFVSKSET
                     RRMEKKKAIRRIKRRSFKDTE"
     gene            791..1048
                     /gene="infA"
                     /locus_tag="PLE39_12610"
     CDS             791..1048
                     /gene="infA"
                     /locus_tag="PLE39_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNE69217.1"
                     /translation="MTKEEGKIKVDGLVIEALPGTQFRVRLDNGHEVLAYLSGKMRKH
                     YIRILLGDHVSMEMSPYDLTRARITFRQRKNTPATPSTTGS"
     gene            complement(1110..2111)
                     /locus_tag="PLE39_12615"
     CDS             complement(1110..2111)
                     /locus_tag="PLE39_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNE69218.1"
                     /translation="MTDLSTTYLGLKLKNPLVASASPLSKKVESAKKLQDAGISAIVM
                     YSLFEEQIIHESLELDHYLTRGTDSFAEALSYLPDSGMYSIGPEKYLSQLTGLKKALN
                     IPVIGSLNGVSKGGWTHYAKQIQDAGADALELNLYFISTDTALTSQELENAQIELVAE
                     IKSAITIPLAVKLSPFITALPNYARRIVEAGADGLVLFNRFYQPDFDLNELEIVHSLD
                     LSTSADLRLPLRWVSILHGKVNADFALTSGVHTSSDVLKAMMSGAKVAMMASKLLQTG
                     EQVIPSMLEELQAWMKEREYVSIEQMQGSMSQKSVKEPAAFERANYMKVLNSFRDLV"
     gene            complement(2108..5674)
                     /gene="nifJ"
                     /locus_tag="PLE39_12620"
     CDS             complement(2108..5674)
                     /gene="nifJ"
                     /locus_tag="PLE39_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNE69219.1"
                     /translation="MKQPIIMIDGNEATASVAHRLSEVIAIYPITPSSGMGEFSDEWS
                     AKGKKNLWGTTPLVIEMQSEGGAAGTVHGALQTGALTTTFTASQGLLLMIPNMYKIAG
                     ELTSTVFHVAARAIASHALSIYGDHQDVMAVRQTGWALLSSGSVQEAQDFAAIAQAAT
                     LKSRVPFMHFFDGFRTSHEVTKIFQLTDEELRQMVDDDLVRAHRARGLSPEHPTLRGT
                     AQNPDVYFQMREASNNYYAATPAIVQEMMDKFAKITGRHYNLFDYSGHPEAERVIIIM
                     GSGGEVVEETVNYLAKKNEKVGVLRVRLYRPFSIEHFLKGLPKNVKSIAVLDRTKEPG
                     ANGEPMYLDIVNALMEGNRSNIKVIGGRYGLSSKEFTPAMTKSVLNELAKPEPKNHFT
                     LGINDDVSNTSLSVDPSFSLESEGMVSCVFFGLGSDGTVGANKNSIKIIGEETENFAQ
                     GYFYYDSKKSGTVTMSHLRFGPKPIRAPYLIEANQANFVACHQYSFLERVDMLKYAKE
                     GGIFLLNSLYGPNEVWDQLPQEVQKDIITKKLKFHVINGYDVAEKTGMGGRMNTIMQT
                     AFFAISGILPRDAAIAEIKHAIEKSYGKRGEAVVRKNFEAVDATVANLYEVKVPAAVT
                     SKMTRRLPVPDEAPDFVKNVLGQIIDSDGDNIPVSAFPIDGTFPTGTTQWEKRNLALE
                     IPVWDADLCIQCGKCVMVCPHAVIRHKVYDEKMLADAPETFKHTASKFKEFSNGYAYT
                     VQVAPEDCTGCTLCVEVCPVKDKRAVGRRAINMEAQPPLREAEAKNWDFFMNIPDLDR
                     KLINPSTIKNSQLLRPLFEFSGACAGCGETPYVKLVSQLFGDRAIIANATGCSSIYGG
                     NLPTTPWAKNAEGRGPAWSNSLFEDNAEFGLGMRLTIDKQKEYARELLKSFETTVSTE
                     LVEAILNANQADEAGIGTQRERVAELKNKLSKSSDSRAKDLISLADNLVQKSVWIIGG
                     DGWAYDIGYGGLDHVLASGRNVNVLVLDTEVYSNTGGQSSKSTPRAAVAKFAMSGKGM
                     PKKDLGLIAMSYGYVYVAKIAMGANDQQTLKALIEAESYDGPSLVIAYSHCIAHGYDM
                     AKGLEQQKLAVQSGHWPMYRYDPRLAMNGQNPLVIESKEPSIPFSQYAYNETRYKMLT
                     QMNEERAEELMKEAQHDAKSRWTLYQQMASMHYGNNNEEKKS"
     gene            complement(5846..6214)
                     /locus_tag="PLE39_12625"
     CDS             complement(5846..6214)
                     /locus_tag="PLE39_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69220.1"
                     /translation="MNSKPMMILIAGPYRSGTNDDPEKIAANVHFMESFALPIFRKGH
                     IPLLGEWFALPLVALAGSRQIGDEPFNEIFHPIAVMLLDKCDAVLRVGGASSGADQMV
                     SVAKEKGAQIFYSLDEIPQA"
     gene            complement(6215..6757)
                     /locus_tag="PLE39_12630"
     CDS             complement(6215..6757)
                     /locus_tag="PLE39_12630"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNE69221.1"
                     /translation="MPFELIKSEPLLQGRAFKIRRDTMKTPDGRETKFDIVEHGGSVV
                     IIPVDGEGNLLFVRQYRHAAGMDMLELPAGTRDGDEPFAECAAREIREETGMEAGTLI
                     QVGAFYLAPGYSTEYMGVFLATDLKHNPLEADDDEFLSVEKIPVRDAIRSAERGELPD
                     AKTLAALLMAKPHLEKYLGA"
     gene            complement(6822..7577)
                     /locus_tag="PLE39_12635"
     CDS             complement(6822..7577)
                     /locus_tag="PLE39_12635"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE69222.1"
                     /translation="MSNKQRGLLIMCVFVISNLLISACQMSASSAPAATPTALPTGQF
                     TSALPTGDNPMNMIAEFAKQTSVAQTTVANGGTPLAPQQTPATTGTVITAQPGTLPTA
                     TPTVGVPSTPTNAAAVATTPAVASTPSGPTTTPVPAGVRPATYTLQNGEFPYCIARRF
                     NVDPDELLSLSGLTSPDLYYPGLKLTIPQSGAFPGDRMLTSHPSTYTVASADENLYSI
                     ACKFGDVDPASLASANGISLSAALTIGQQIKVP"
     gene            complement(7643..9631)
                     /locus_tag="PLE39_12640"
     CDS             complement(7643..9631)
                     /locus_tag="PLE39_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69223.1"
                     /translation="MMNINQLKKFPALLFALITLVAYGLLVPFTGFYWDDWPFAFIAK
                     FLGPAEFNPAFAPFRPFLGPIFYFTTSLIPPVPLYWQIFALIIRFLIGITALWMFKQV
                     WPQRTNTALFAALLLLVFPGYSQHWVALTHINQELIPFIFYLLSFGFSFKGLHEQKTR
                     YIVIAILFQFCGIFPTEYFFGIEGIRFFLLFAFMQGSLLERFMKTIKAWWPFLLVWIL
                     NAVWLVYYYRFGPYTSYEVTATQSLTALSFLKEALDALWKAGIYIWFQILPLALTSLP
                     SPASILSLGLILLSFAFLALYLNPDTETTKTDPSFAISLILIGIPAILLGRLPSLAAG
                     LPLTLQSSYDRFMVSMMIGGSLFLLGMMELLFGKFRIKNYIFALVIALGIGQQFFNAN
                     IFRRDWENQRDIYWQMAWRIPALEPNTLLLTHQMPIDYETDISFTAPINWMYGTETSI
                     QNGLPYLMLYTEKRLGGTTLPSFAPDTPVTFPYRTRTFNGSTSQAIVFYMPQNGCLKV
                     FNPARGDLEIYSKLPDVLVDAIPLSDPSRIITDPSTPATPMFFPEPEHGWCYYYTKAE
                     LAQQNNDHQTVMALADEAIALGYNPVDPYEWFVFIEAYAMQGDVQTAEKLSTNALASD
                     ARIRKSLCAVWEQVQVNGPEGSADEIQQVILTLKCNSQ"
     gene            9767..10870
                     /locus_tag="PLE39_12645"
     CDS             9767..10870
                     /locus_tag="PLE39_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69224.1"
                     /translation="MKKILHVLFLVCILMLQACGESPSPTAAVDVPEPTAIASAAAVV
                     TAGVVVPPTEATFIAAPPVTHVVIPSAGTSDRATAHDNENSLFFDTKKVKTGDEYFRN
                     RFERPFTAVDMNYLPDLDIVNFSINSDENFFYVKISMVGLDASTQTLNGSYGIEIDRN
                     ADGRAELLLTTRPPYSTEFTADNVAAYLDLNSNVGGGVINRPDSFGDDGFEAIIFDLN
                     KGIYPENDPDFLWVRQATDGELPAVEIAFARWMFKDNGKEAFMWSVISNANGLDPSKM
                     NYHDHYTPEQAGAANTDDPNYPLKELSALDNTCRVPLGFQASGTELLGCYMKSGDVEE
                     KATPVPDGPNSTCSIFAELCNRTKLTYQLYIPR"
     gene            complement(10906..11883)
                     /locus_tag="PLE39_12650"
     CDS             complement(10906..11883)
                     /locus_tag="PLE39_12650"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNE69225.1"
                     /translation="MREKILELLSGKQVNTQPVFSGLIHITAEGLQHEGLIFHEIHKD
                     AAKMAKAAASTFKLTGIPSAALPLDLCAPAEMMGAELNYYENMEYQFPQVKKALVESA
                     RDIKAVQFGAGRLELICEAISLVKNDIGGEAVISGLIPGPYTLLLYVCNPANMFMEMK
                     KEPQVVRDALTHLASFLAKVGQAYLNAGADFITIHEMGGSPGFIGPARFEQFILPALK
                     DLHANLPAPKVLSVCGKTDAAMHLLAQTGADAISVDQLNHLAASRSVLQDMLLFGNID
                     PVETLWQGDPAKVAESVVGAKEAGVNAIWPGCDLVPQTPLANLQSMKSA"
     gene            complement(11973..12854)
                     /locus_tag="PLE39_12655"
     CDS             complement(11973..12854)
                     /locus_tag="PLE39_12655"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNE69226.1"
                     /translation="MNKFLERLNSGEILVADGATGSNLQKMGLKPGKPPEDLIIDDPD
                     TILKLASSFAQAGSDIILTCTFGGTRMRMKDSKYQDRTPEVNIRAAEIARKAASLNGG
                     LVAGSMGPIGALVKPYGPVDADDVKATFAEQAKALADGGVDLLLIETMFSFEETTAAF
                     EGARSTTDLPIVISFSYDRGTRTMMGVKPKDVMKRYSEMGATLIGANCGTTLENMEAV
                     IKEYAATNSNIPLWVKPNAGVPHMDIETEQGVYDMGPEDMAAFSKKYVELGAKVVGGC
                     CGNTPEHIAAIVKAVKN"
     gene            complement(12950..14980)
                     /locus_tag="PLE39_12660"
     CDS             complement(12950..14980)
                     /locus_tag="PLE39_12660"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HNE69227.1"
                     /translation="MNKHNIILQPSGRRGQVEEGMSVRSAARELGVEIESICAENATC
                     GKCMVLIEEGRFEKYNIDSKRDNVSPVTTEERAYLERRPKLLKDKGWEIGQVRLSCQA
                     KVCGDVLINVPEESRGNKQIVRKSASSREIEVKPAIRKYLVSMSPPTLERPIADWERL
                     AKGLETSMALVRGTEEKLPRWHELQIDYHCLKTLSPTLRAAKWNVTVSVWQDKEVIEV
                     QPGYVEDSYGAAVDIGSTTVALYLCNLRTGEMLAAESEMNPQIVYGEDVMSRIQYTIE
                     HKDGLEKLHKAIISTLNKLLKQAAKTAKIKTEEILEMVLVGNSTMHHILLNLHPKDLG
                     LAPFVPAIHKSMDIKARELGLHINSSGNIHVLPTIASFVGADTSAMIVAEEPHKQDEN
                     WLLIDVGTNAELVLGNRKRLVCTSTPTGPALEGAHVEYGMRAAPGAIERVHIDEKTLE
                     PKYKVIGVDGWNTEHAEFKGHVKGICGSAIIDSVAELFRAGIVDSRGKFKKGLNSNRI
                     REGESGWEYVIAWAEETSIGRDIPMTQQDVRQIQLAKAALFTAARTLLKRSGMQSPDK
                     IILAGGFGSYIDKEKAMLIGLIPDCDLENVYAVGNAAGDGARIALLNVEKRNEIDGVT
                     RKVDRFELPTDPEFQNNFMLATSFPHMSEPFEHIAHLIPNRVADPMAKNFIK"
     gene            complement(15039..15329)
                     /locus_tag="PLE39_12665"
     CDS             complement(15039..15329)
                     /locus_tag="PLE39_12665"
                     /inference="COORDINATES: protein motif:HMM:NF025142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HNE69228.1"
                     /translation="MSKYRVFYWKEIPASIVVEGDGRTIKKQLSQKVQNKIDAYAMVV
                     GLTSTEDYSAQYKRGAWVEREGAPEDVAEALLAELEAEAAKVEIPKREKTSE"
     gene            complement(15411..16451)
                     /locus_tag="PLE39_12670"
     CDS             complement(15411..16451)
                     /locus_tag="PLE39_12670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE69229.1"
                     /translation="MKRLWVRLSLMISGVLFFMFFMQFLVITLEQSGMRSDLDGPPDA
                     PPAEIQGRLIEFMAFSIAVGLGGGVVIARVISTPINGLVNAAHQIGKGELSTRVPARG
                     SQEMIELSETFNKMAADLQHAETLRNNLMADVSHELRTPLTVMEGNLRAVLDHVYPLD
                     ETVIANLYSQTHHLIRLVNDLRELSLAESGHLKLEKIPTDIQALIAETLQAIEPLSVE
                     KNIKMENEAVPTSDVTVDPFRIRQALFNLLSNALRHTPDGGTISIKTTSDDHAITISI
                     QDTGDGLTTDELASVFNRFYRADRSRSRETGGSGLGLAIVKAIIESHGGSVQAQSNGK
                     GTGSVFSICIPK"
     gene            complement(16451..17155)
                     /locus_tag="PLE39_12675"
     CDS             complement(16451..17155)
                     /locus_tag="PLE39_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE69230.1"
                     /translation="MSQRILVVDDDRSIVKVICSYLEQSGYQTLAAYDGEMALHQIRR
                     ERPDLVILDLMMPNRDGWEVTQIVRADKALAPTPIIMLTARVEDTDKIVGLELGADDY
                     ITKPFNAREVVTRVKALLRRVKLDQSPHATPHILTCGGLKLDLDQRTLSVDAEPVELT
                     RTEFNLLEAFLSNPGYTLTRDDLLEKSMGYAYEGMGRALDTHIRNLRRKIEPDPDNPT
                     YIQTVYGVGYRLTGDK"
     gene            17297..18253
                     /locus_tag="PLE39_12680"
     CDS             17297..18253
                     /locus_tag="PLE39_12680"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE69231.1"
                     /translation="MKSNFVSSVSKIALAVLALVVVLVATGCSGLSVMPGGSASFASV
                     DPTYADLAYATQSDAQKLDLYIPEGSGPFPVVIMVHGGGFMFGDKADGAGLTGVDQLL
                     AAGYAVASINYRLSGEAQYPSQIYDAKASVRFLRANAEKYNLNPDKFAAWGASAGGNL
                     VSLLGTTCGVEELEGADLGNAEQSSCVQAVVDWFGPIDFLKMDEQFAGTSCEQSHNAA
                     NSPESKLVGAEIQTVPDLVATTNPMNYITADDAPFFIENGTADCNIPPIQNKNLADAL
                     SAVIGADNVTYVSLEGAGHGGSQFETAENLKLVIDFLDKNLK"
BASE COUNT         4313 a         4389 c         4791 g         4768 t
ORIGIN      
        1 agtgggaaac tcgccctaag attagatccc tgtattcccg taagggagta agaccgcagg
       61 tagactacct gcttaatagg cagcatgtgt aagcacagta atgtgttaag ctaagctgta
      121 ctaatggtcg agggcttcct tctcgtgata cggagaagtt ggtacttttc agaaaactac
      181 atcatatatt tgctatttgg tgacaatctt gtgaaattgt cccttggtga tttgagcgac
      241 gtgggtacac ccggtcccat tccgaacccg gaagttaagc atgtcagcgc cgatggtact
      301 tggagggcga cctcctggga gagtaggtca ttgccaaggg gcttttcttt ttaatattta
      361 tcatgcataa aatcttctca ttcatgcatg tatcgtgtat aatgcgaatg cagttttggc
      421 atggagtttt tctccatgaa attttgagga gtgtgaagcg catcttttga tcttatggtc
      481 atctagttcg tttcaggtct tgttataaag tcctcggctg attctgcgta acggccgatt
      541 ttttttggac atttttattc agaaagtagg tgatttcatt ggctacagtt aatcttcgta
      601 acggcgaatc ccaagattca ttgctcaagc gttttcgtaa gaaaatcgtc aagagtggaa
      661 ttctgagcac cattcgcaat aagcgctggt ttgtctccaa gagcgagacc cgtcgtatgg
      721 aaaagaagaa agcgattcgc cgtatcaagc gtcgctcctt caaggacact gaataaatca
      781 agaggcgtgt atgacaaaag aagaaggcaa aatcaaagtt gatggtcttg taattgaggc
      841 attgcctggt actcaattcc gtgtgcggct tgacaatggt catgaagtac tggcttatct
      901 ttctgggaaa atgcgcaagc attatattcg tattctcctt ggagatcatg tttccatgga
      961 aatgtcaccc tatgatttga cccgtgcccg catcaccttc cgtcagcgca aaaacacgcc
     1021 tgcaacacct tctactactg gcagttaatt tccaaaaaaa acaagtcctc cattttcatg
     1081 gaggacttgt tttttttaaa gtttttttct tataccaaat ctctgaaaga attcagcacc
     1141 ttcatgtaat tggcgcgttc aaaagccgct ggttctttga cagatttctg actcatgctg
     1201 ccctgcatct gttcaatgga aacatattca cgctctttca tccacgcttg caactcctct
     1261 agcatggacg ggatgacctg ttcgccggtt tgaagtaact ttgacgccat catggcgact
     1321 tttgcaccag acatcatggc tttgagtaca tcgctgctag tgtgtacgcc gcttgtaaga
     1381 gcaaaatctg cattgacttt tccatgcaga attgagaccc aacgcagggg caggcgtaaa
     1441 tcagctgaag tgctaagatc caggctgtga acgatttcca gctcattcag gtcaaaatca
     1501 ggctggtaga accgattgaa taatactaga ccatcggcgc cagcttcgac gatgcggcgg
     1561 gcatagtttg gcagggcagt aatgaaggga cttaatttca ccgccaatgg aatggtgatc
     1621 gccgatttta tctcagccac cagttcgatt tgggcgtttt ccaattcctg agaggtcaat
     1681 gctgtgtcag tggaaataaa gtaaaggtta agctccagcg catctgcacc ggcatcctga
     1741 atttgttttg cgtagtgggt ccagccgcct tttgagacgc cgttcaagct gccgataaca
     1801 gggatgttca gggctttttt caggccagtc agttgactca ggtacttctc agggccgatg
     1861 gagtacatgc cgctgtcagg cagataggac agggcttcgg caaatgaatc ggttccacga
     1921 gtcaggtagt ggtccagttc gagcgattcg tggatgatct gctcttcgaa gagagagtac
     1981 atcacaattg cagaaatgcc tgcatcctgc aattttttag ccgactcgac cttttttgag
     2041 aggggagatg ctgaggctac cagcggattc tttaatttca ggccaagata ggtcgtagaa
     2101 agatcggtca tgattttttc tcctcattat tgtttccata gtgcatcgaa gccatttgct
     2161 gataaagcgt ccaacgggat tttgcatcgt gctgggcttc cttcatcaat tcttctgcac
     2221 gttcttcatt catttgcgtg agcatcttgt agcgggtttc gttgtaggca tactgcgaga
     2281 atggaatgct cggttccttg gattcgatga caagcggatt ctggccgttc attgccagac
     2341 gcggatcgta gcgatacatg ggccagtggc ctgattgcac cgcaagtttc tgttgctcga
     2401 ggcctttggc catgtcgtaa ccgtgagcga tgcagtgact gtatgcgatg acaagggacg
     2461 gcccgtcata ggattctgcc tcgatcaatg ccttgagtgt ctgctggtcg ttggctccca
     2521 tggcgatttt tgcaacatac acatacccat aggacattgc aataagcccc agatccttct
     2581 ttggcattcc cttgccgctc atggcaaatt tggcaactgc tgcacgtgga gtggatttgc
     2641 tggattggcc gccagtgttt gaatacactt ccgtatccaa aaccaacaca ttgacgttgc
     2701 gcccgcttgc aagcacgtgg tcaagaccgc cgtaaccaat gtcataggcc cagccatcac
     2761 caccgatgat ccagactgat ttctggacta gattgtctgc aaggctgatc aggtctttag
     2821 cgcggctatc gctcgatttg ctgagtttgt ttttcaattc ggcaacccgt tcacgttggg
     2881 tgccaatgcc tgcttcatct gcttgattgg cgttcaaaat ggcttcaacc aactcggtgc
     2941 ttacagtcgt ttcgaacgat ttgagcagtt cacgtgcata ttctttttgc ttatcgatcg
     3001 tcaggcgcat acccagaccg aattcggcat tatcttcgaa aagtgaattg gaccaggcag
     3061 gcccgcgccc ttcggcattc ttggcccatg gggtggtggg gagattacca ccataaatgg
     3121 aggaacatcc cgtggcattt gcaatgatgg cgcggtcacc gaacaattgt gagacgagtt
     3181 tcacatacgg ggtttctcca caaccagcac aggcaccgct aaactcaaat aacgggcgca
     3241 gcaactgtga attcttgatg gtggaggggt tgatcaactt gcgatcaagg tcggggatgt
     3301 tcatgaagaa atcccaattc ttcgcttccg cttcacgcag aggtggttgg gcttccatat
     3361 taatggcgcg tcggccaacc gcacgtttat cctttacagg gcaaacttcc acacacaagg
     3421 tgcagcctgt gcaatcttcg ggggctacct gaactgtata cgcatagcca tttgagaatt
     3481 ccttgaactt cgaagcagta tgcttgaagg tttcgggggc atctgccagc attttctcat
     3541 catatacttt gtggcgaatg accgcatgtg gacaaaccat cacgcacttg ccgcactgga
     3601 tgcacagatc tgcatcccat actggaatct caagcgccag attgcgtttt tcccactgcg
     3661 ttgtgccggt ggggaaggta ccgtcaatgg ggaacgcgga aacggggata ttatcgccat
     3721 ctgagtcgat gatctgccca agcacgttct tgacgaaatc aggcgcttca tccgggactg
     3781 ggagtctgcg tgtcatcttt gaggtgaccg ccgctggaac tttgacctca taaagattcg
     3841 ccactgtggc atctacagct tcaaagtttt tccgtacaac cgcttcgccg cgcttgccat
     3901 aactcttttc gatggcatgt ttgatctcgg caattgccgc atcacgcgga agaatgccgc
     3961 tgatggcaaa aaaggccgtc tgcatgatgg tattcatacg tccacccatg cctgtttttt
     4021 cagcgacatc gtacccgttg atgacgtgga atttgagttt cttggtaatg atatccttct
     4081 gcacttcctg tgggagttga tcccatactt cgttgggacc atacagactg ttgagcagga
     4141 agatgccacc ctctttggca tatttcagca tatccacacg ctcgaggaag gaatattgat
     4201 ggcaggcaac gaaatttgcc tgattggctt cgatcagata cggtgcgcgg atgggtttgg
     4261 gcccaaagcg caaatgagac atggtcaccg tgccagattt cttcgagtca tagtagaagt
     4321 aaccttgggc aaagttttca gtctcttccc cgatgatttt gatcgagttt ttgttcgcac
     4381 ccactgtccc gtcggagccg aggccgaaaa acacacaact gaccatgcct tctgattcaa
     4441 gtgagaaaga ggggtccaca ctcaaactgg tgtttgaaac gtcgtcattg ataccgaggg
     4501 taaaatgatt cttgggttcg ggtttggcca actcattcaa aaccgatttt gtcatggcgg
     4561 gggtgaattc cttggacgaa agaccataac gcccaccgat gactttgata ttgctgcgat
     4621 taccttccat aagcgcattg acgatatcga gatacatcgg ttcgccattg gcaccaggtt
     4681 ccttggtacg gtccagcaca gcaatggact tcacattctt gggcaggccc ttaaggaaat
     4741 gctcaatgga gaatgggcga tataaacgca cccgcagtac cccaactttt tcgttctttt
     4801 ttgcaagata gttgacggtt tcttctacta cttcaccgcc agaacccatg atgataatga
     4861 cacgttcggc ttctgggtgg ccgctgtaat cgaagaggtt gtaatggcgg cctgtgattt
     4921 tggcgaattt atccatcatt tcctgcacga tggcaggtgt ggcggcataa taattattcg
     4981 aagcttcccg catttggaaa tacacatctg gattttgcgc agttccgcgc agggtgggat
     5041 gctcggggct gagtccacgg gctcgatggg cacggaccag gtcatcgtcc accatttgac
     5101 ggagctcttc atccgttagt tgaaatatct tggttacctc gtgtgaagtt ctgaacccat
     5161 caaagaaatg catgaaaggg acacgcgatt ttagagtggc tgcctgcgca atggctgcaa
     5221 aatcctgagc ttcctgaacc gagccagaag agagcaaagc ccagcccgtc tgacggacag
     5281 ccatcacatc ttgatggtcg ccataaatgg aaagcgcatg ggatgcaatg gcacgtgcgg
     5341 ccacatggaa gacggtgctg gtcaattcgc ccgcgatctt gtacatattt gggatcatta
     5401 acaataagcc ctgagatgcg gtgaaggtgg ttgtcagtgc acctgtttga agcgccccat
     5461 gtacggtccc agctgcgccg ccttcagatt gcatttcaat gacgagtggg gtggttcccc
     5521 aaaggttttt ctttcctttg gctgaccatt catcagaaaa ttcacccatg ccagaggatg
     5581 gggtgatcgg gtaaatggcg atgacttcgc ttagacggtg ggcaacgctt gcggtcgcct
     5641 cattaccgtc gatcataatg atgggttgtt tcattcttgc tcctttgtaa gcaggcttac
     5701 acctgggaat ttcttcaatt tacaagtgta tcccccttac aactctaagg ttagttatta
     5761 tccgcttaaa tatatgtgct tgttgtcaca ttttggacac gaatggtgga aataaaaagc
     5821 ggcggaatat ccgccgcttt gttgattatg cctgaggtat ttcatcaagc gaataaaata
     5881 tctgtgcccc tttttccttc gccacactta ccatttgatc tgccccgctc gaggccccac
     5941 ccacgcgaag gacggcatcg catttatcaa gaagcattac agcaatggga tgaaagattt
     6001 cattgaaagg ttcatcgccg atctgcctgg accccgccag agcaaccagt ggaagcgcaa
     6061 accattctcc caatagggga atgtgcccct tgcggaaaat cggtagtgca aacgactcca
     6121 taaaatggac attggctgcg atcttttcag gatcatcatt ggttcctgag cggtatggcc
     6181 ctgcaatcag aatcatcatg ggcttgctgt tcatttatgc tcctaaatat ttttcaaggt
     6241 gaggtttcgc cattaataag gccgccaatg ttttggcatc tggaagttcg cctcgttctg
     6301 cgctgcgaat tgcatctcga acagggatct tctccaccga tagaaattca tcatcatctg
     6361 cttcaagcgg attatgtttc aaatcggtgg ccagaaagac acccatgtat tcagtggaat
     6421 atcccggtgc aagataaaac gcgccgactt gaatcagtgt gccagcctcc atgcctgttt
     6481 cttcgcggat ctcacgcgcg gcacattcgg caaagggttc atcaccatcg cgtgtgccag
     6541 cagggagttc aagcatatcc atccccgccg catggcgata ttggcggacg aaaagcaggt
     6601 taccttcccc gtccacgggg atgatcacca ctgaaccgcc gtgttcaaca atgtcgaatt
     6661 tggtttcgcg gccatctggt gttttcattg tgtctcggcg aatcttgaag gcacgtccct
     6721 gtaacaaggg ttcagatttg atgagttcaa aaggcatatg ggtctccttg gtagcaaaaa
     6781 agcccaagca tgcgcctggg cttttttatc agcatgatcg attacgggac tttgatctgt
     6841 tgaccaatgg tcaaagcggc cgagagggaa attccattgg cagaggcgag cgaggcggga
     6901 tcaacatcgc cgaacttgca ggcaatgctg tacagatttt catctgcaga ggccacggta
     6961 taggtggaag gatgcgaagt caacatgcgg tcaccgggga acgcgccgct ctgcgggatg
     7021 gtcagcttaa gaccagggta gtacaggtcg gggctggtaa gtccgctcag gctgagcaat
     7081 tcatcggggt ctacattgaa acgacgggca atacaatatg ggaactcacc attctgcagg
     7141 gtataggttg cagggcgcac cccggcaggg acgggggtgg tggtggggcc gcttggggta
     7201 gaggcaacgg caggagtggt ggcaacagca gctgcattgg tgggagtgct cggtacacca
     7261 acagtgggag ttgcggtggg cagggttccc ggctgggcag tgataacagt gcccgtagtt
     7321 gcaggggttt gctgcggggc aaggggagtg ccgccgtttg ccacagtagt ttgagccact
     7381 gaagtttgtt ttgcaaattc agcgatcatg ttcatggggt tgtcaccggt gggcagggca
     7441 gaggtgaatt gtccggtggg cagtgcagtg ggggttgcgg ccggtgcgga agatgccgac
     7501 atctgacagg cagagatcag caagttcgat attacaaaga cacacatgat tagcaagccg
     7561 cgttgtttat tactcatagt ttctcctgtt tcgatatact cagtgggcga tggtagcaca
     7621 tgaactttaa agcgtcaagc gattattgcg aattgcactt aagtgtcaat attacctgtt
     7681 gaatctcatc tgcgcttcct tctggaccgt tgacctgcac ctgttcccag acagcgcaca
     7741 agcttttgcg aatgcgtgca tctgatgcaa gcgcgttagt ggatagtttt tctgcagtct
     7801 gaacatctcc ctgcattgca taggcttcaa tgaagacgaa ccattcataa ggatcaacgg
     7861 ggttatagcc cagcgcaatg gcttcgtccg ccagtgccat cacggtttga tgatcattat
     7921 tttgttgagc taattcggct ttggtgtagt agtaacacca gccgtgttca ggctcgggga
     7981 agaacatggg tgttgcaggt gtggatgggt cagtgatgat gcgcgatgga tctgaaagcg
     8041 gaatggcatc cacaagaaca tcagggagtt ttgagtagat ctccaaatct ccacgagcgg
     8101 ggttgaacac tttcaggcat ccattttgag gcatgtaaaa gacgatggct tgtgaagtgg
     8161 acccattgaa tgtgcgggtg cggtaaggga atgtgaccgg ggtatctggt gcaaaagatg
     8221 gcagggtggt gccgccaagg cgcttttcag tgtagagcat caggtaggga aggccgtttt
     8281 gaatggatgt ctctgtgccg tacatccagt tgatcggtgc ggtgaaggaa atgtcggtct
     8341 cgtagtctat gggcatttgg tgggtgagga gcagggtatt cggctccagg gcagggatgc
     8401 gccatgccat ttgccagtag atatcgcgct ggttttccca atcgcggcgg aagatattgg
     8461 cgttgaagaa ttgctgtccg attccgagcg cgatgaccag agcgaagatg taatttttta
     8521 tgcggaattt cccaaaaagg agttccatca tacccagaag gaacaaactg ccaccgatca
     8581 tcatagaaac catgaagcgg tcataagagg attgcagggt gagcgggagc cctgctgcca
     8641 aggatggaag cctgcccagc aaaatggcgg gaataccgat gagaatgagc gaaatggcaa
     8701 aagacggatc ggtttttgtg gtttctgtgt cggggtttag ataaagggcg agaaatgcaa
     8761 aagaaagcag aatcaggccg aggcttaaga tggaagcggg cgatgggagt gaggtcaatg
     8821 ccaggggcag aatctggaac cagatgtaaa ttccagcttt ccagagagca tccaacgctt
     8881 cctttaaaaa ggaaagtgcg gtcagtgatt gtgtggctgt gacttcgtaa gaggtgtacg
     8941 ggccgaatct gtaataatat acgagccaca cggcattgag aatccagacg aggaggaagg
     9001 gccaccacgc tttgattgtt ttcatgaagc gctcaagcaa gctgccttgc atgaatgcga
     9061 aaagcaaaaa gaaacgaata ccctcgatgc cgaaaaaata ctcggtgggg aagatgccgc
     9121 agaattgaaa aaggattgcg atgacgatgt atcgggtttt ttgttcgtgc agccccttga
     9181 agctgaagcc gaaggagagc aggtaaaaaa taaaaggaat aagttcctgg ttgatgtggg
     9241 tgagtgccac ccagtgctgg ctgtaacccg ggaagacgag gaggagcaat gcggcaaaca
     9301 gagcggtgtt ggtgcgttgc ggccatacct gtttgaacat ccacaaggcg gtgatgccga
     9361 tcagaaagcg gataatgagt gcaaatatct gccagtaaag cggtacaggc ggaatcaggc
     9421 tggtggtgaa ataaaagatc ggcccaagga atgggcggaa gggagcaaaa gcagggttga
     9481 attcggctgg gccgaggaat ttggcgatga aggcgaaggg ccagtcatcc caataaaacc
     9541 cggtaaaggg aaccagcaag ccataggcga caagcgtgat caaagcgaac aacaaagcag
     9601 ggaatttttt tagttgattt atattcatca tgtttttgta attataagat agcagacctt
     9661 tcgtttttgt taggtataat gattttattc taaacctatt tcctacaaga tgaaattata
     9721 gttaatgata tattgatgcc ctgcatctca aaacgaggaa atgaagatga aaaaaatact
     9781 tcacgtatta tttttggttt gtatattaat gctgcaggcg tgtggagaat cgccttctcc
     9841 aactgccgca gtggatgtcc ctgaacccac cgccattgca agcgcagcgg cggttgttac
     9901 tgctggtgtt gtcgtgcctc caaccgaggc aacttttata gctgccccgc ctgtgaccca
     9961 tgtagtcatt ccatcggcag ggacgagtga tcgtgccacc gctcatgata acgagaattc
    10021 cctgttcttt gacaccaaaa aggtcaagac aggagatgag tattttagaa atcgcttcga
    10081 acgtccattt accgctgtgg atatgaatta cctgccagac cttgatatcg tgaatttctc
    10141 gatcaatagt gatgagaact tcttttatgt gaaaatttca atggtgggac tcgatgcttc
    10201 cacccaaacg ctgaatggct cctacgggat cgaaatcgac cgtaatgccg atggccgcgc
    10261 tgaattgtta ttgaccaccc gcccgcccta ttcaactgaa tttaccgccg ataatgtagc
    10321 cgcgtatctc gatctcaaca gcaatgttgg cggcggcgtg atcaaccgcc ctgattcatt
    10381 cggcgatgac ggatttgagg ccatcatttt cgacctaaac aaagggatct acccggaaaa
    10441 cgaccccgat tttctctggg tgcgccaggc tacagatggc gaattgccag cagtggaaat
    10501 tgccttcgca cgctggatgt ttaaagacaa tgggaaggaa gccttcatgt ggagtgtgat
    10561 ttcaaacgcc aacgggcttg acccatccaa aatgaattat cacgatcact acacccctga
    10621 gcaggcaggc gcagccaata ccgatgaccc caattatccc ctcaaggaac tttcggcact
    10681 ggataatacc tgccgagtgc ctctcggttt tcaggcttca ggcactgaac ttttaggctg
    10741 ttacatgaag agcggcgatg tggaggaaaa agccacaccc gtgcccgatg gccccaactc
    10801 cacctgtagt atttttgcag agttgtgtaa ccgtacgaaa cttacttatc aactttacat
    10861 tccacgctaa accaagatga taaaaaaagc gcctgacgaa atccgtcagg cgcttttcat
    10921 tgattggaga tttgccaacg gcgtttgcgg gacaaggtca caacctggcc agatggcatt
    10981 cactcccgcc tctttcgcac cgacaactga ctcagcaact ttggcaggat ctccctgcca
    11041 gagagtctca acagggtcaa tattgccaaa caaaagcata tcctgcaaaa cggaacgcga
    11101 tgcggcaaga tgattcaact gatcgaccga aatggcgtcc gcacctgttt gggccagtaa
    11161 atgcatggcg gcatccgttt ttccacatac ggataatacc ttcggcgcag gaaggtttgc
    11221 atgcaggtct ttcagtgcag gcaggatgaa ttgctcaaac ctggcagggc caatgaaacc
    11281 cggtgacccg cccatctcgt gaatggtgat aaagtcggcg cctgcattga ggtaggcctg
    11341 cccaactttt gcaaggaagg aagcaagatg ggtgagggca tcccgtacga cctgcggctc
    11401 ttttttcatt tccatgaaca tgttggcagg attgcatacg tacagcagca gggtatatgg
    11461 cccagggatc aaacctgaga tgaccgcctc tccgccaata tcattcttta ccagactgat
    11521 cgcctcacaa atgagctcta accgcccagc accaaattga accgccttga tatctctggc
    11581 tgattcaacc aatgctttct tcacctgcgg gaactgatac tccatgtttt catagtaatt
    11641 caattcagca cccatcattt cagcgggcgc acacagatcc aacggcaaag cggcggaggg
    11701 aatccccgtc agcttgaatg tgctggcggc ggcctttgcc atttttgccg cgtctttgtg
    11761 aatttcgtgg aagatcagcc cttcatgctg caacccctca gcggtgatat ggatcagccc
    11821 gctaaaaaca ggctgggtat tgacttgttt ccctgacaac aactctaaga ttttttcacg
    11881 catggttgca tccttttaca aaatacggat tatgaattcc gtgtaccgca actcataatc
    11941 cgtacctgat tcctgtctat tttttgttgc tactaattct ttacagcctt cacgattgca
    12001 gcgatatgtt caggggtgtt cccacaacaa ccgccgacca ctttggctcc cagctcaaca
    12061 tatttctttg agaaggcggc catgtcttca ggacccatat catacacacc ttgttcggtc
    12121 tcgatatcca tgtgtggaac gcctgcattg ggtttgaccc acagagggat atttgaattg
    12181 gtggctgcat attccttgat taccgcttcc atgttttcca acgtggttcc acagttggcg
    12241 ccgatgaggg ttgctcccat ttcggagtag cgcttcatta cgtctttggg ttttacgccc
    12301 atcatggtgc gtgtgccgcg gtcgtagctg aatgagatca caatggggag gtctgttgta
    12361 gagcgcgccc cttcgaaggc agcagtggtc tcttcaaaag agaacatcgt ttcgatcaat
    12421 aacaggtcca caccgccatc tgcgagggcc ttggcttgtt cggcaaaggt tgcttttaca
    12481 tcgtccgcat ccacggggcc gtagggtttg accaaagcgc cgatcgggcc cattgaacca
    12541 gcgaccagcc ctccgttaag ggaggccgcc ttgcgggcga tctctgccgc gcggatgttc
    12601 acttccggag tgcggtcctg atatttcgaa tctttcatcc gcatgcgtgt gccgccaaag
    12661 gtgcaggtca agataatatc cgagcctgcc tgtgcgaagg aagaagcaag tttgagaatc
    12721 gtgtcagggt catcaatgat cagatcctcg ggtggcttgc caggcttgag gcccatcttt
    12781 tgtagattcg aacctgttgc tccatccgca acaagtattt ccccgctgtt caatcgttcc
    12841 aaaaacttat tcatacctgc tctccttgtt tccacgaagg gacacaaagg tcacaaaggt
    12901 attagatctt ttcctttgtg acccttcgtc tccttggtgg tgaaaaaaat tattttatga
    12961 aatttttcgc catcgggtcc gccacacgat tggggatcaa atgtgcaatg tgttcgaacg
    13021 gctcgctcat gtgcgggaag cttgtcgcaa gcatgaaatt attttggaat tcaggatcgg
    13081 tgggcagttc aaagcgatct actttgcggg ttacaccatc gatctcgtta cgcttttcaa
    13141 cattcaacag tgcgatgcgt gcgccatcgc cagcggcatt tccaactgca tacacatttt
    13201 ccagatcgca atcggggatg agcccgatca gcatggcttt ctctttgtca atgtaacttc
    13261 caaagccgcc agcgaggatt attttatcag gagattgcat cccgctgcgt tttaataatg
    13321 tccgtgccgc ggtgaacaaa gctgcctttg ccagttgaat ctggcgcaca tcctgctggg
    13381 tcatggggat gtcgcggcca atggaagttt cctcagccca ggcgatcaca tactcccagc
    13441 cgctttctcc ctctcggatc cgattcgaat ttaacccttt cttgaacttg ccgcgagaat
    13501 caacaatgcc tgcccggaac aattctgcca ccgagtcgat gatcgctgag ccgcagattc
    13561 ccttcacgtg ccccttgaac tcggcatgtt ccgtgttcca accatccacg ccgatgactt
    13621 tgtattttgg ttcaagtgtc ttctcgtcaa tatggacgcg ctcaattgcg ccaggcgcag
    13681 cccgcattcc atactccaca tgagcacctt caagtgcagg gcctgtgggg gtggaggtgc
    13741 agaccagccg cttgcgattt ccaagcacca attcggcgtt ggtgccgacg tcgatcaaca
    13801 gccagttttc atcctgtttg tgcggttcct ctgccacgat catggcgctg gtatcggcgc
    13861 cgacaaagga ggcaatggtg ggcagtacat ggatattgcc cgatgaattg atgtgcagac
    13921 ccaactcacg cgccttgata tccatggatt tgtgaatggc agggacaaat ggcgccagcc
    13981 ccaaatcctt cgggtgcagg ttcaacaaaa tatggtgcat ggtggaattt cccaccagaa
    14041 ccatttccag aatttcctcg gttttgattt ttgcggtttt ggctgcctgc tttaataact
    14101 tgttcagagt ggagatgatg gccttgtgta gtttttccag gccatccttg tgttcgatgg
    14161 tgtattgaat gcgcgacatc acatcttcgc cgtacacgat ctgcggattc atttcggatt
    14221 cagcagcgag catttcgcct gtgcgcaaat tgcacaggta caaggccacc gttgttgagc
    14281 cgatatccac agcggcgccg tagctgtctt ccacatatcc aggctgcact tcgatcactt
    14341 ccttatcctg ccagaccgaa accgtgacat tccattttgc ggcgcgcagg gtgggggaga
    14401 gggttttcag gcagtggtaa tcaatttgca attcgtgcca gcgggggagt ttttcttctg
    14461 tgccgcgaac cagtgccatg gaggtttcga ggcccttggc gagacgctcc caatcggcta
    14521 tggggcgctc cagcgtgggc gggctcatgg aaaccagata tttacggatc gcaggtttga
    14581 cctcgatctc gcggctgctt gcgctcttgc ggacgatctg cttatttccg cggctttctt
    14641 caggcacgtt gatcagcaca tcgccgcaga ccttggcctg acatgaaagc ctcacctgac
    14701 cgatctccca gcccttgtct ttcaatagtt tgggtctgcg ctcaagatat gcacgttcct
    14761 ccgtagtaac aggggatacg ttatcacgct tcgaatctat gttgtatttt tcaaagcggc
    14821 cttcttcgat caagaccatg cactttccgc aggtggcatt ttcggcacag atcgattcga
    14881 tctccacacc cagttcacgg gctgccgaac ggacagacat gccttcttcg acctgaccac
    14941 ggcggccaga gggttgcaaa atgatgttgt gtttgttcat aatattgaca atagacggcg
    15001 gacgaccgac cacgatcagc ggctatcgtc cacggtcatt actccgaagt tttttcccgc
    15061 ttgggaattt ctacctttgc tgcttctgct tccagttccg ccagaagtgc ttcggccaca
    15121 tcttcaggtg cgccttcgcg ttcgacccag gccccgcgct tgtattgcgc gctgtaatct
    15181 tcagtggagg ttaacccaac caccatggca taggcatcga tcttgttctg caccttttgt
    15241 gagagttgct ttttgatggt gcggccatct ccttctacga cgatggaagc gggaatttcc
    15301 ttccagtaaa aaacgcgata tttgctcatg tcagttcctt taaattatat ataatttgtg
    15361 tcagcgattt tacgtgaggt tggcatagcg gtcaatgtaa aaaataaaaa ttattttgga
    15421 atgcagattg aaaagacgct gcctgtaccc tttccattac tttgtgcctg cacactgccg
    15481 ccatgcgatt cgatgatcgc tttgacaatg gccagcccca atccgctgcc gcctgtttcg
    15541 cgtgagcgcg atctatcggc gcgataaaag cgattgaaaa cagaagcaag ctcatcggtc
    15601 gtcagaccgt cgcctgtgtc ctgaatcgaa atcgtgatcg catgatcatc gcttgttgtt
    15661 ttgatcgaaa tcgtcccgcc atcaggggtg tgccgcaagg cgttggatag cagattaaac
    15721 agcgcctgcc gaattcgaaa tggatccacg gtcacatcag acgttggaac tgcttcgttt
    15781 tccattttga tatttttttc aactgagaga ggttcgatcg cctgaagtgt ttcagcgatg
    15841 agcgcctgga tatcggtggg gattttttcc aacttcaaat gacccgactc ggcgagtgac
    15901 aactcgcgca gatcgttgac cagccgaatc agatgatggg tttgactgta aagattcgcg
    15961 atcaccgttt catcgagcgg atacacatga tcgagcacgg cgcgcaaatt gccttccatc
    16021 acggtgagcg gtgtgcgtag ttcgtgcgag acatcagcca ttaagttgtt gcgcaatgtt
    16081 tctgcatgtt gcagatcagc cgccattttg ttgaaggttt ctgaaagctc gatcatttcc
    16141 tggctgccgc gggcgggtac gcgtgtggag agttcgccct tgccgatctg atgcgcggca
    16201 ttcacaagcc cgttgatcgg cgtgctgatc acacgcgcaa tgaccacacc tcccccaagc
    16261 ccgactgcaa ttgaaaaagc catgaactcg atcaggcggc cttgaatttc ggcgggcggc
    16321 gcatcaggcg ggccgtcgag gtcggatcgc attccactct gttccaatgt gatcaccagg
    16381 aactgcatga agaacataaa aaacaacacc ccgctgatca tcaaactgag gcgcacccat
    16441 aatcgcttca ttatttatct cctgtcaaac gatagccaac gccatagaca gtttggatgt
    16501 aggtcgggtt gtctgggtcg ggctcgatct tgcggcgcaa attgcggatg tgtgtgtcga
    16561 gtgcgcgtcc catgccttcg taggcgtagc ccattgattt ctcgagcagg tcatcgcggg
    16621 tgagtgtgta accggggttg gatagaaatg cttcgagtaa attgaactcg gtgcgggtga
    16681 gttcgacggg ttctgcatcc acacttaatg tgcgctgatc gagatccaat ttcaggccgc
    16741 cgcaggtgag aatgtgaggt gtggcatgtg gagattgatc caatttgacg cggcgcagca
    16801 gggcttttac gcgtgtgacc acttcgcggg cattgaatgg cttggtgatg taatcgtcgg
    16861 cgcccagttc cagcccgacg atcttgtctg tatcttccac gcgggcggtg agcatgatga
    16921 tgggggtggg cgcaagcgct ttatctgcgc ggacaatttg ggtgacttcc caaccgtcac
    16981 ggtttggcat catcaggtcg aggatcacca gatcggggcg ttcgcggcgg atctggtgca
    17041 gcgccatttc gccatcgtag gcggcaaggg tttgataacc agattgttcc aggtatgagc
    17101 agatcacttt tacgatggaa cggtcatcat ctacaacaag aattctttgt gacattttct
    17161 acctcttatc gagtcatcat agcatatcat tctcaaggaa atctcaagaa tacaaacagg
    17221 tcatcttcag gatttcttga caagtctttg tgatactggg ggagttcgat caatcatttt
    17281 gtaaatggag acaccaatga aatctaattt tgtaagttct gtttcgaaga tcgcgctggc
    17341 tgtgctggcg ctggttgttg tgctcgttgc cacggggtgc agtgggttgt cggtgatgcc
    17401 gggtggttct gctagttttg cttccgttga cccaacttat gcagacctcg cttatgcaac
    17461 ccagtctgat gcgcagaagc tggatctgta tattcccgaa ggcagtggac ctttcccagt
    17521 tgtcattatg gttcatggcg gcgggtttat gttcggtgat aaagccgatg gcgcggggct
    17581 gacgggtgtg gatcaacttc tcgctgctgg ctatgctgtg gcgagtatca actaccggtt
    17641 aagtggggag gcgcagtatc cttcgcagat ctatgacgca aaggcatctg tccgcttttt
    17701 gcgcgcgaat gcagagaagt acaatctcaa ccccgataag tttgcggcgt ggggcgcttc
    17761 tgcgggcggg aatttggtgt cgctgcttgg cacgacctgc ggtgtggaag aattggaagg
    17821 cgcagacctc gggaatgcgg agcaatcgag ttgtgtgcag gcggtggtgg attggtttgg
    17881 cccgatcgac ttcttgaaaa tggacgaaca gtttgcgggg acaagctgcg aacagagtca
    17941 taatgccgcc aattctcccg aatcaaaact ggtcggcgca gaaattcaaa ccgtgcctga
    18001 tctggttgca accaccaacc cgatgaatta catcactgcg gacgatgcgc ctttcttcat
    18061 tgaaaacggc acagccgatt gcaatattcc gcccattcaa aacaagaatc ttgcagatgc
    18121 gctcagcgcg gtgatcggcg cggataacgt gacctacgtt tcactcgaag gcgcggggca
    18181 tggcggctct cagtttgaga cagccgagaa cttgaaactg gtgattgact ttttggataa
    18241 gaatttgaag tagttttttg g
//
LOCUS       DAOQJC010000082        18243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1428_length_18243_cov_8.997416, whole genome shotgun sequence.
ACCESSION   DAOQJC010000082 DAOQJC010000000
VERSION     DAOQJC010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18243
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1428_length_18243_cov_8.997416"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..74)
                     /locus_tag="PLE39_12685"
     tRNA            complement(2..74)
                     /locus_tag="PLE39_12685"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38..40),aa:Glu,seq:ttc)
     gene            complement(128..682)
                     /locus_tag="PLE39_12690"
     CDS             complement(128..682)
                     /locus_tag="PLE39_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69232.1"
                     /translation="MTLDTSPRTYRFLPADIMTSGYRVVGKIMITNNGVMGMMNDATH
                     SAMEVNDARLARLHMPTKLVDHFELIRMMKRQIHAVCLARREDLGPLALVRGGYASVV
                     EHPVRIATQMFEVEGTMELPGRFDFTAIMTDGTRTFLPVLNATLTAILIPNLRVESAS
                     ILVNRNLIDIMALINQRVKPEIQP"
     gene            complement(704..1000)
                     /locus_tag="PLE39_12695"
     CDS             complement(704..1000)
                     /locus_tag="PLE39_12695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HNE69233.1"
                     /translation="MSDNEYTPRSFPCNECHSGILQPRHITYFTWLGNELITVPHFPA
                     WVCDVCGKREYDEKAISWLNMILDPNAGKPNAKSRRPLPRPKLRTNNPRPAHDS"
     gene            complement(1010..1645)
                     /gene="lepB"
                     /locus_tag="PLE39_12700"
     CDS             complement(1010..1645)
                     /gene="lepB"
                     /locus_tag="PLE39_12700"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNE69234.1"
                     /translation="METLQHEEQPQSPIEGQVEGQIEGQPTQEAPAQEKIKWGRFFVD
                     ILETLVLAVILFFGINAVSARVRVDGYSMRPTLDDGEFVLVSKFNYKLSNVQRGDIIV
                     FHFPMDPKQELIKRVIGLPGDRVHVKDGVVYINNQALDEPYIAAPPAYTSMWNVPEDR
                     LFVLGDNRNDSSDSHSWGYLPFENVVGKAVLIYWPPPMWDVIEHKDLLVAQ"
     gene            complement(1704..2423)
                     /locus_tag="PLE39_12705"
     CDS             complement(1704..2423)
                     /locus_tag="PLE39_12705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE69235.1"
                     /translation="MNWHTRYTQQANWTRELRAYLFEQAGMDRAKRILEVGCGTGAIL
                     STLNTSATRHGLDLEPASLAECRIHAPAALLSCADAHSLPYPDQSFDIVYCHFLLLWV
                     KDPLQVVREMARVGRTVLALAEPDYSQRVDEPATLKSLGVWQTESLRRQGADPSFGGM
                     LAETFHQVGIKLIETGPIQSQVVMRSAEDWETEWAVLQTDLEGIAQREEIQKMKVLDE
                     IARKNGDRTLYVPTYFAWGKT"
     gene            complement(2420..2983)
                     /locus_tag="PLE39_12710"
     CDS             complement(2420..2983)
                     /locus_tag="PLE39_12710"
                     /inference="COORDINATES: protein motif:HMM:NF012330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HNE69236.1"
                     /translation="MTKLPIPECYWVEENRLLAGEYPGHHDPEIMRRRIDAFLEAGAD
                     TFFDLTQAHELVPYEATLQEEARIYGIDASYQHFPIRDYGIPTANTMTAILNAIDEAT
                     SAGHCVYVHCWGGVGRTGLVVGCHLVRHGFTNQQAIAQVDTLFKTRPNRIFHPRSPES
                     DEQVDFILNWWEDATELHRNNKRFCEG"
     gene            complement(2985..4001)
                     /locus_tag="PLE39_12715"
     CDS             complement(2985..4001)
                     /locus_tag="PLE39_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69237.1"
                     /translation="MKKNFVAVLALLLFSLACQTLFPKNTTPPDNNVTAEPTQRGTPS
                     PIVCTDDSCINACLERINNTLETRRFDSIGGDYAGAEANFDLVKYQVENGAISDPEIL
                     YVPKEFKPFQDDLQAQQTVWKYASSLLTENELKWITEYVVFTDGESNTLAWVNNKDDL
                     DLSRWQLGVDIIDAADPVDLTATLIHEFGHLITLNSEQFTPTDYYYGWNQNNAACPQF
                     TSPEGCSTPDSYINLFYQNFWIDIFDEWLETVENPQVDSDEEFDALRRAFYSKHSEQF
                     VREYAATNIREDMAESFMHFVLTPQPNGNNPVEQKMLFFYQFEELTTLRRQIIQNVCS
                     YTQE"
     gene            complement(4032..4328)
                     /locus_tag="PLE39_12720"
     CDS             complement(4032..4328)
                     /locus_tag="PLE39_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69238.1"
                     /translation="MELQKGGKFIMYEYVISLLVITFKRNSNIYFVKADESAVVKGLP
                     FTLLSGLVGWWGIPWGIIYTIQAIFTNLSGGKDVTTDVLSAIRARQSAQNVNPA"
     gene            complement(4384..5514)
                     /locus_tag="PLE39_12725"
     CDS             complement(4384..5514)
                     /locus_tag="PLE39_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE69239.1"
                     /translation="MNFIQKIFTKTAPLPAGTHHMQAMHDEKPYRLHLRLHKDGSGLL
                     ILNAATVIQLNSTAAEYAYHFIKGTSPDEAAKQVAARYRVDKKTALADFNNFADQIQT
                     LISTPDLDPASFLNFERAQPHSTESTLRLDCAITYRLPESTHATYAPVKRVDRELTTT
                     EWQSILDKAWQAGIPHIIFTGGEATLREDLPQLIGHAEKNGQVCGLLTDGLKLADKNY
                     FETLLQTGLDHVMLILQPDEPKSWKAIETIVPQDIFLTVHLTLTKENINEAQNTLQKL
                     AYLDVQNISLSAADASLLDEMLELQDTASSLRLTLRWDLPVPYSAANPVSIETVDEET
                     PSGAGKTWMYVEPDGDVLPAQGEPDKILGNFLRDDWRTISKQ"
     gene            complement(5536..6546)
                     /locus_tag="PLE39_12730"
     CDS             complement(5536..6546)
                     /locus_tag="PLE39_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69240.1"
                     /translation="MEICYLIAFPDTDDGQNPAPERFKGLKDAPYFQPIDIDLVTLGE
                     ETLIIEGHAVQVFRQRYDGCVQMIECHFDLKDPFAASVLQERAKIQSALESKFVPTHY
                     RQNGLFEEYTILLVKEAKPTPDKWIEKNALALTKFIRSQREVFDKSEIEEILISRTHY
                     SAVELTLIDWEGAIIIAPNEDYQSDIALLKIGNYQLLRYRMLDQSIENTLDKINETFF
                     EKKRGLRPTRGVIRQIAEHKLEVMLDFERAEQNLLLIGDWYTAKLYEAIQSEFYLKDW
                     KENVKTKLDNLENIVQTIRDNFSLSLESVWGRIEMLGWMIMLIGYAYLFLADMGILKI
                     GK"
     gene            complement(6561..6902)
                     /locus_tag="PLE39_12735"
     CDS             complement(6561..6902)
                     /locus_tag="PLE39_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69241.1"
                     /translation="MNDHAWIKEFPAAITVCDDEGIILEMNDKAAKTFEKDGGYKLVG
                     SNVLDCHNDPSRGKLERLMSAREKNVYTIEKNGVKKMIFQSPWYKDGIYAGFVELSLE
                     IPFEMPHFIRS"
     gene            complement(6958..8355)
                     /locus_tag="PLE39_12740"
     CDS             complement(6958..8355)
                     /locus_tag="PLE39_12740"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HNE69242.1"
                     /translation="MSLNFSSLKNILSLPFQQAPKADAAETETRLYHYVHENEMEKSR
                     IHLRIDPDGTGTLIVNASSVMHLNPTAAFMAWLILEGKTEDERVQALRARYSVSKKQA
                     REDLAEFNYQLEEMIRPDGACPVHELDLETKMPFSARPSAPYRMDLALTYRCNNDCAH
                     CYNARERNFPELTTQQWFKIIDQLWELGVPHIVFTGGEATLRNDLPELIHHAESNGQI
                     TGLNTNARRLMDMDYVKKLVNAGLDHVQITVESSVPEIHDEMMRAKGAFRQTIAGLNN
                     VLASELYVMTNTTMLRTNVHTIPATLDFLSDLGVPTIGLNALIYSGHGLTVGTGLLES
                     ELQPILNIATQKTAEHGQKLIWYTPTQYCQFDPTAHNLGVKGCTAALYSMCIESNGNV
                     LPCQSYYHPLGNMLTDSWDSIWNHKLSTQLRERQNLPFKCETCSLVAECGGGCPLQFE
                     NHREKFGVNLPVLTL"
     gene            complement(8339..8872)
                     /locus_tag="PLE39_12745"
     CDS             complement(8339..8872)
                     /locus_tag="PLE39_12745"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNE69243.1"
                     /translation="MLGAIFKKAPHTPPHLFVPNALYMLTASTYRSEPFMQTTERKLN
                     WIKTFLTSAEMYKWEVVAWVVLDNHYHAVLKAPDSADNLPKFVASYHRFTARNWNEEE
                     QASGRKVWWNYWDTCIRSEYDYYNRLRYTFWNPVMHSLVKDPNDYPFSSYKVFLSKWK
                     VKFNFTNMEEVKNVPEF"
     gene            complement(8881..10548)
                     /locus_tag="PLE39_12750"
     CDS             complement(8881..10548)
                     /locus_tag="PLE39_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69244.1"
                     /translation="MKRKLLLLSFILILVLAAFSNASAQDAPYYFEVNKEVVHVYWNP
                     DGTMSVDYTWYFNNLPGSHPIDFVDVGMPNYNFNTSTIVAESDGMDLMVTTQDYQGSG
                     SGFAVVMGSRTIQPGDKGILHVFVPNISSVLYNDANDANYASAVFAPTWFGQEFVSGN
                     TDFTMTFHLPPNMNPEEPRWHEAPSGFPETPQAAYDSENRVTYTWYAPAATAYNYYSF
                     GASFPRIYVPAEAIYTPPPEPLFNIDEDTIFSLLCGGFFFFIFFISPAIAAITGRRRK
                     MQYLPPRVAIEGHGIKRGLTAVEAAILMEQPLDKVMTMILFSVVKKGAAQVETRDPLK
                     LSVAAEQPATLHPYEKDFIKAFAVDEKRSRVLQDMTVALIKSVSEKMRGFSRKETVEY
                     YKSITEKAWAQVEAAGTPEVKSQKMDESLEWTMLDKDYDDRARRVYNGPVYVPTWWGR
                     YDPSYRRISTPTTVSGPTISSTPRPSSSGQALPGADFAASMVTGVQTFSQKVIGNVQS
                     FTERVTGATNPLPKSSSSGGRSGGGGRSCACACACAGCACACAGGGR"
     gene            complement(10572..10976)
                     /locus_tag="PLE39_12755"
     CDS             complement(10572..10976)
                     /locus_tag="PLE39_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69245.1"
                     /translation="MANKTFLYIGAGILLLIGLCLLGYGLLNVIGSTSAQGSSSWLTF
                     GLGFSCVGVFFLAGGIGLIVFASRQKGPAGSGDQITYKIDLPAQTKVEQMKCNSCGGA
                     LKADNVKMIAGAPTVECPYCGTVYQLTEEPKW"
     assembly_gap    11033..11132
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11133..11761)
                     /locus_tag="PLE39_12760"
     CDS             complement(<11133..11761)
                     /locus_tag="PLE39_12760"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE69246.1"
                     /translation="MITWIIGSAVVLVLLFLLDREIYRYEGIHIGPRLQAWLYDRWAE
                     KYDHDKKDGQLQDVEMLAKPLLTKLTEVPEPFILDVATGTGRLPFVVASQPEFTGHII
                     AVDISHGMLKQAAKKIAPHQSRVELMKYDSFPLPFPDETFDVVSCVEALELIGEKQAA
                     IQELARVLRPGGTLLTSRGTEESGRGIVVISAEMLTSLLKTSGFEDIEIT"
     gene            complement(11796..13049)
                     /locus_tag="PLE39_12765"
     CDS             complement(11796..13049)
                     /locus_tag="PLE39_12765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE69247.1"
                     /translation="MMPPRSTGMRTFTMIWFGQIISLLGSAMTWFAFTIWVWQKTEQA
                     SSLAAINTLAFLPSILLMPIAGTFVDKWERKTTLILSDLGSVTATLIALLLYHAGSLA
                     LWHIYLLSLFAGIFTAFQYPAYVAATTALVSKDDYARAQGMVGLAQAASTIFAPMIAA
                     ALLPSIGLSGIMTIDLLTFVFAFSILLWIKFPLRSDLTPAPVTQTGFAREVSFGFRSI
                     FANPTLRALTILFVVANFFLAIGATLLAPTILSHTANSEGALAAILSVGAAGGLIGGG
                     LLSVWGGPKRKVNGILLGGVAACLIGISLFGLARSVLIWGIASFFFSFFEPFVEGSNT
                     ALWQSTVEINAQGRVFSARQLLTQIPYLIGMAGSGWLAELGIARFTGTQNGIHLSTTL
                     VLAGLAGSAVFIIGYFVPPIREIQE"
     gene            13198..15144
                     /locus_tag="PLE39_12770"
     CDS             13198..15144
                     /locus_tag="PLE39_12770"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE69248.1"
                     /translation="MKREVELHFEYENMSRRDDDLLRHELEERRKSLLDKVVTVQDSS
                     FSLEEHEWLARSFPVLSILAPVMSTNEGKIEFPGDPMCLYSALSYAVDQAVKTRKLGL
                     SEGAPYNDLCPQWGALPSADYRAAVDVNGIRDYHGVLLNTDQTVFDPRVWNGLVKKYF
                     VEKVLLVMRPKVVLISAVSPAHRYAIDIARVVREHLPDCLIVLGGRHVDETIHFDLVN
                     RKVVFEPSSAVVKIQEGTVEPVFDFLIGGDGYYALDVLMKAISISMDLETKSTQIDAV
                     MKTLSTIAPLFDPIPGNSVIVGMDKRKSVHAWVINSRIKLDLSVLPSPYQAFAVRALF
                     PIFQRDGRVLRTAHFMVTNSCTYHCYFCSEGVTVVGTFRSFNSDGIQRALEKVVEYIS
                     YGAEALFFDDSIFWGGNFGNVIHFCREWLHLRELAKKSPSKMIKLFGYEVEAQKVLDL
                     VWGAQFTVDLLASRRIPEEAMLALHEMKSAGCVYIYMGIESMSAPVIDKVHKNVNRKQ
                     AWDERVRAALGMARLAGITVGSSVLFGLDGETMETIEETISKVEELLVEDILSIASPN
                     ILTYHPNTAITAQHGMKDKIDYHSIGIENRPPYVYFEEAFPSVVSRNLSEEQIWFIHE
                     QATRRWGVKRNLNPMPEVILREGG"
     gene            complement(15146..15511)
                     /locus_tag="PLE39_12775"
     CDS             complement(15146..15511)
                     /locus_tag="PLE39_12775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE69249.1"
                     /translation="MNEHLALIIEDDEDLSEIFGQALVAAGYTIEIIRDGAIAQERLK
                     EITPTVITLDMHIPHISGDILLNQIREDDRLTNTRVVVTTADAQMGENSRGQADLVLI
                     KPITFSQLRDLTARLKPQN"
     gene            complement(15523..17220)
                     /locus_tag="PLE39_12780"
     CDS             complement(15523..17220)
                     /locus_tag="PLE39_12780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE69250.1"
                     /translation="MFDRLLAISANDPDDARRKKLLNILLFGLGILTIILIIVMVISL
                     LSQNAQEGTLLVVYGGIALLLGMGIIYLLNRSLPGKIAAILFLFLITSVMIFSDSPHE
                     IANGRSLFIFTIPIIMASVLLTPVSSFLFATISSVIISALAISIQEDPNSPAIAGYFL
                     VALIAWLSSRNLEQALSELRSINLNLDKVVEEKTRELASTLSRELILAGRNQAILDSI
                     ADGVIVFDEFNTAILANPALSRLADLPKDGLLHRKITDFIQNDLISPTTRGTLLGLLE
                     NPQASATGIRMDWGNKTLSTSFAQVHSSDGKNIGTVAVFRDVTHEVELEKMKNTFIGI
                     VSHELRTPLNAILGYAEMLKEGVYGPANEKQQNISLRIMTNTRRLLDIVSDLLDEVQI
                     EAGKLKIQMIACKPSDLLENLHSVMDKITSDKKLQFITELDPSMPPVLKGDPKRLQQI
                     MVNLSNNAVKFTEKGSIRVKLSRLDDQNWQIQTSDTGEGIPEEAKEYIFESFRQVKES
                     ATRQHGGVGLGLSIVKQLVELMKGKINVESEIGGGSIFTVTLPLIVPIEAETLLEKE"
     gene            complement(17251..>18243)
                     /locus_tag="PLE39_12785"
     CDS             complement(17251..>18243)
                     /locus_tag="PLE39_12785"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69251.1"
                     /translation="GLKINRNITAVFSAEMAGTDSTDLLDFDHLQEPNGSVGCSPSST
                     AYFARYLKPGDEKALSYLRNISKDGGVPNFAPFDIFERCWVLWNLSLLESHTPQETDL
                     IQGHLDYISKRWVPKTGIGFASDCAMYDSDDTSVMQRVLLRFGREADIEAVLSYEEEE
                     YFRCVQHEASPSVGVNIHILGALKAAGFEKKHPSVQKILKFLYNTRTDKGYWFDKWHV
                     SPYYITSHVIIDCLEYDLKLCQQAVDWILNTQNANGAWGYFGISTAEETAFCLQALKV
                     WRNSGNAVPSGRIEMAHMWLSENSKPPYPWMWIAKTLYYPELIIQSCIMTALTL"
BASE COUNT         4082 a         4145 c         5100 g         4816 t
ORIGIN      
        1 gtgcccccac ggggattcga accccggtcg tggccttgaa agggccgcgt cctaggcctc
       61 tagacgatgg gggccttttt tagagcgggc gaattctatc atcccattct gtgtcagtca
      121 agcgatttta gggttgaatt tctggtttca cccgttgatt aatcagcgcc ataatgtcga
      181 tcaagttgcg gttgacaagg atgctggcac tctcgacacg caggtttgga atgaggattg
      241 cagtcagtgt agcgttcagc acaggcaaaa aagttcgcgt gccatctgtc ataatggcag
      301 tgaagtcgaa gcgtcctggt aattccatgg ttccctccac ttcgaacatt tgagttgcga
      361 tccgtaccgg gtgctccacc acgctggcat atccgccgcg caccagagcc agtggaccca
      421 ggtcttcacg gcgggccaga cacacagcat ggatctgacg cttcatcatg cgaattaatt
      481 caaaatggtc cacaagcttc gttggcatat gcaaacgagc cagccgcgca tcattcactt
      541 ccatggcaga atgagttgcg tcattcatca tccccatgac accgttgttt gtgatcatga
      601 tcttgccgac cacacgatac cctgaggtca tgatgtctgc tggaagaaaa cgataggtgc
      661 gcggagaagt atcaagtgtc atggagtgaa aattccctct cggttacgaa tcgtgggcag
      721 ggcggggatt gttcgtacgg agtttggggc gcggtaacgg gcggcgcgat tttgcattcg
      781 gcttgcctgc attggggtcg aggatcatgt tcagccagct gattgctttt tcatcgtact
      841 cgcgttttcc acacacatca caaacccagg cggggaaatg cggcaccgtg atcaactcat
      901 tgccaagcca tgtaaaatac gtgatgtgac gcggctgtag aatgccagaa tgacattcgt
      961 tacaggggaa tgaccgtggt gtgtattcgt tgtcactcat ggggcatcct tattgtgcaa
     1021 ccagtaagtc cttgtgttcg atcacgtccc acattggggg cggccaatag atcaaaaccg
     1081 ccttgccaac cacattttca aagggcaaat aaccccagga atgtgaatca gatgaatcat
     1141 tgcgattatc gcccaaaacg aacagacgat cttcagggac attccacatg ctggtataag
     1201 cgggaggggc ggcaatatag ggttcgtcca atgcctggtt gtttatgtaa acaacgccat
     1261 ctttcacgtg aacccgatcg cccggcaggc cgatcactct tttgatcaac tcctgtttag
     1321 ggtccatcgg gaagtggaac acaataatat cgccgcgctg cacattgctt aatttgtaat
     1381 tgaatttgct aaccagaaca aattcaccgt catcaagggt ggggcgcatg ctatacccat
     1441 ccacccgtac gcgcgctgaa accgcattga taccgaaaaa taaaataacg gccagcacca
     1501 aagtttccag aatatccaca aaaaagcggc cccactttat cttttcttga gcaggcgctt
     1561 cctgtgtagg ctgcccttct atttgaccct caacctgccc ctcaattggg ctttggggtt
     1621 gttcttcgtg ttgtaatgtt tccaatcatg cctccaatgt atgggcaaaa ttatacttcc
     1681 ctttattcga agtctgccac tccttaagtc ttaccccagg caaagtatgt aggcacatac
     1741 aaggtcctat ctccattttt tcgcgcaatt tcatccaaaa ccttcatttt ttggatttct
     1801 tccctttggg cgataccttc caaatctgtt tggagcacgg cccattcagt ttcccaatct
     1861 tcggcagaac gcatcacaac ctgactttga atgggtcctg tttcgatcaa tttgatgcca
     1921 acctgatgga atgtttcggc cagcattccg ccgaatgatg ggtcagctcc ctgtcggcgt
     1981 aatgactctg tctgccatac gcccagtgat ttgagcgtgg caggttcgtc aacacgttga
     2041 ctgtaatcag gttcggccaa tgctaacacg gtgcggccca cgcgagccat ttcgcgaaca
     2101 acctgcaatg gatctttcac ccacaaaagc aggaagtgac aatagacaat atcgaacgac
     2161 tgatcggggt aggggagaga gtgtgcatcg gcacagctta gcaaggctgc cggggcgtgg
     2221 attctgcact cggcgagcga agcaggctca aggtccagac catgacgcgt ggctgaggtg
     2281 ttgagggtgc tcaaaatggc acccgtaccg cagcctactt cgagaatccg tttggcacga
     2341 tccatcccag cctgttcaaa aagataggcc ctcaattctc gggtccagtt tgcttgttgc
     2401 gtgtagcgcg tgtgccagtt catccttcgc agaatctttt gttatttcgg tggagttcgg
     2461 ttgcgtcttc ccaccaattg aggatgaagt ccacttgttc gtcgctttct ggcgagcggg
     2521 gatggaagat gcggtttggg cgggttttga acagtgtgtc cacctgagct atggcttgtt
     2581 ggtttgtgaa gccgtggcgg acaaggtggc agccaacgac gagcccggtg cgtcccacgc
     2641 cgccccagca atggacatac acgcagtgac ctgcgcttgt agcttcgtct atggcattga
     2701 ggatggcggt catggtgttt gcagtgggaa tgccataatc gcggatgggg aagtgttgat
     2761 aggaggcgtc tatgccgtat atgcgtgctt cttcctgcag ggtggcttcg tatgggacga
     2821 gttcgtgcgc ttgtgtgagg tcgaaaaagg tatccgcgcc cgcttcgaga aaggcatcga
     2881 ttctgcgacg catgatctct gggtcgtgat ggcccgggta ttcacctgcc agaaggcggt
     2941 tttcttcgac ccagtaacat tcggggatgg ggagtttggt cattttattc ctgagtgtat
     3001 gaacagacgt tttggatgat ctggcggcgc agggtggtca gttcttcaaa ttggtaaaag
     3061 aagagcattt tttgttccac gggattgttt ccattgggtt ggggggtgag gacaaaatgc
     3121 atgaacgact cggccatatc ttcacggatg ttggtggcgg cgtattcgcg gacaaattgt
     3181 tcgctgtgct ttgagtaaaa cgctcttcgt aaggcgtcga attcttcatc tgagtctact
     3241 tgaggatttt cgacagtttc gagccattcg tcaaatatat cgatccagaa gttttgatag
     3301 aacaggttga tataggaatc gggtgtactg catccttccg ggctggtgaa ttgcgggcag
     3361 gcggcattgt tttgattcca gccgtagtaa tagtcggtgg gtgtgaattg ttccgagttt
     3421 agggtgatga ggtgcccgaa ttcatgtatc agggtggcgg tcaggtctac aggatcggcg
     3481 gcgtctatga tatccacacc tagctgccag cgtgaaagat caagatcgtc tttattgttg
     3541 acccatgcca gggtgttact ttcaccatcg gtgaaaacta cgtactcagt gatccatttg
     3601 agttcgtttt ctgtaagaag ggatgatgca tatttccaga ccgtttgttg ggcttgcagg
     3661 tcgtcttgaa agggtttgaa ttctttgggt acatagagta tttcggggtc actgatggcc
     3721 ccgttttcca cctgatattt gacaaggtca aaattcgcct ctgcaccggc gtaatccccg
     3781 ccgattgagt cgaagcggcg ggtctcgagg gtgttgttga tcctttcaag acatgcgttg
     3841 atgcaggagt cgtcggtgca aacgatggga gatggggttc cacgttgtgt gggttcagcg
     3901 gtgacgttgt tatcgggggg tgttgtattc tttgggaaga gggtttggca tgcaagagag
     3961 aagaggagca acgctaaaac tgccacgaag ttttttttca tgggttacct ttttaatgtc
     4021 cctgctggat tttaggctgg gttgacgttt tgtgcagatt gtctggcgcg gattgcggag
     4081 agtacatcgg ttgtcacatc ttttccgccg ctaaggttgg tgaagatcgc ttgaatggtg
     4141 tagataattc cccagggaat gccccaccaa ccgacgagtc ctgagagcaa ggtgaatggc
     4201 agacccttta ctacggcact ttcatctgct ttgacaaagt agatgttgga gttgcgcttg
     4261 aacgtgatga cgagcaggga aatgacatac tcgtacatga tgaactttcc gcccttttga
     4321 agttccattt gtagctccat gctgcccata ttttctgtgc cgatgatttt cacttttgcg
     4381 ctcctattgt tttgaaattg tacgccagtc atctctcaaa aagttaccga ggattttatc
     4441 gggttcgccc tgagcgggca gaacatcgcc atcgggttcg acgtacatcc aggtcttgcc
     4501 tgcaccggaa ggagtttctt catccacggt ttcgatggag acgggattgg ccgcagagta
     4561 gggcacgggc aggtcccagc gtagggtgag gcggagggaa ctggctgtat cttgaagttc
     4621 aagcatttca tcgagcagac tggcatctgc ggcactgagt gaaatgtttt gcacatcgag
     4681 ataggcgagc ttttgcaggg tgttttgggc ctcgttgatg ttttctttgg tgagggtcaa
     4741 atgaacggtg aggaaaatat cctgcgggac gatggtttcg atggctttcc atgatttggg
     4801 ttcgtcaggc tgcaggatga gcatgacgtg gtcgaggcct gtttgcagaa gggtttcgaa
     4861 atagttttta tcggcgagtt tcaaaccatc ggtcagcagg ccgcagactt gcccgttctt
     4921 ttcagcgtgg ccgatgagtt gcgggaggtc ttcgcgcaag gtggcttctc cgcctgtgaa
     4981 gatgatgtgc ggaatgcctg cctgccaggc tttatcgaga atggactgcc actctgtggt
     5041 ggtaagttca cggtctacac gtttgacggg agcgtaggtg gcgtgagtgg attcgggtag
     5101 tcggtaggtg atggcgcaat caagcctgag ggttgattct gttgaatggg gctgtgcccg
     5161 ttcaaagtta agaaaggagg cggggtcaag gtcaggcgtt gagataagcg tttggatctg
     5221 gtcggcaaag ttgttgaaat ctgccagcgc ggtctttttg tccacacggt agcgggcggc
     5281 aacctgtttt gcggcttcat caggggaggt gcctttgatg aagtgatagg cgtattcggc
     5341 ggcggttgag ttgagctgga tgacagtggc tgcgttaagg atgagcaggc cagagccatc
     5401 tttgtgaagg cgcaaatgca ggcggtaggg cttttcgtcg tgcatggctt gcatgtggtg
     5461 agttcccgcg gggagcggcg cggttttggt gaagatcttt tgaatgaagt tcatgtcaac
     5521 tcctgttccg tgtgtttatt ttccgatctt gaggatgccc atatctgcga ggaagaggta
     5581 ggcgtacccg atgagcatga tcatccagcc gagcatttca atgcggcccc atacgctttc
     5641 aagcgatagg gagaaattat ccctgatggt ttggacgatg ttctcgaggt tatcgagttt
     5701 tgtttttacg ttttctttcc agtctttgag gtagaactcg ctttgaatgg cttcatacag
     5761 tttggcggtg taccaatcac cgatgaggag caggttttgt tcggcgcgtt cgaaatcgag
     5821 catgacttcg agtttgtgtt ctgcgatctg gcggatgacg ccacgggttg ggcgtaatcc
     5881 gcgtttcttt tcgaagaatg tttcgttgat cttatcgagc gtgttttcaa tggactgatc
     5941 gagcatgcgg tagcgcagga gctggtagtt gccgattttc aacaaggcaa tgtccgattg
     6001 gtaatcttcg ttgggggcga tgatgatggc gccttcccaa tctatcaggg tgagctccac
     6061 ggctgagtaa tgtgtgcgtg agatcaggat ctcttcgatc tcgcttttgt cgaacacttc
     6121 gcgttgtgag cggatgaatt tggtgagggc cagagcgttc ttttcgatcc atttatcggg
     6181 ggtgggcttg gcttctttta cgagcagaat ggtgtactct tcgaagagtc cgttttggcg
     6241 gtaatgggtg gggacgaatt tgctttcaag ggcgctttgg atcttggcac gttcctgcag
     6301 gacagaggcg gcaaatgggt ctttcaggtc gaagtgacat tcgatcattt gcacacaacc
     6361 atcatagcgc tgacggaaaa cctgtacggc gtggccttcg atgatgaggg tttcttcgcc
     6421 gagggtgaca aggtcaatgt cgatgggttg aaaatacggc gcatctttca gccccttgaa
     6481 gcgctcaggg gcggggtttt gaccatcgtc tgtgtcgggg aaggcgatca ggtagcagat
     6541 ttccatgaga ggtaaaggtg ttaggaacgg atgaagtgtg gcatttcgaa tggaatctcc
     6601 agagagagtt ccacgaagcc cgcatagatg ccatctttat accatggaga ttgaaagatc
     6661 atctttttta ccccgttctt ttcgatggtg tagacgtttt tctcgcgggc tgacatcaac
     6721 cgttcaagtt taccccgtga tgggtcgttg tgacaatcca acacattcga cccgacgagt
     6781 ttgtatccgc cgtctttttc aaaggttttg gcggctttgt cgttcatctc aaggatgatg
     6841 ccctcgtcat cacagacggt gatggcggca gggaattctt tgatccaggc gtggtcgttc
     6901 atgagactcc aggggaaggg atgaagggtg aagagaatgt ccgcttcacc ctttcggcta
     6961 caaggtcagt acaggcagat tcactccaaa tttttcacga tggttttcaa attgaagcgg
     7021 gcatccgccg ccgcattccg cgacgagtga gcaggtctcg catttgaagg gcaggttttg
     7081 tctttcgcgc aattgagtgg agagtttgtg gttccagatc gaatcccacg agtcggttag
     7141 catgttgcca agcggatgat aataggattg acagggaagg acattgccgt tcgattcgat
     7201 gcacatactg tacagtgcgg ccgtgcagcc ttttacgccg aggttgtgcg cagtggggtc
     7261 aaattggcag tattgagtgg gggtgtacca aattagtttt tgtccatgct cggcggtttt
     7321 ttgggtggcg atgttgagga tgggttgcag ttcgctttcg agcagacctg tcccaacggt
     7381 caggccatga cccgaataga tgagggcgtt gagaccgatg gtggggactc ccaggtcgga
     7441 gagaaaatcc agcgtggcag gaatcgtgtg gacgttggtg cgaagcatgg tggtgttggt
     7501 catcacatac aattcggagg caaggacatt gttcaggcca gcaatggttt ggcggaatgc
     7561 gcctttggcc cgcatcattt catcatgaat ttcaggcacg ctggattcga ccgtgatctg
     7621 gacatggtca agccctgcat tgaccaattt tttgacatag tccatatcca tcagacggcg
     7681 cgcgtttgtg tttagacctg tgatctgacc attgctttcg gcatggtgaa tcagctctgg
     7741 caggtcgttg cgcagtgtgg cttcgccgcc tgtgaaaacg atgtgcggca cacccaattc
     7801 ccaaagttga tcgatgatct tgaaccattg ctgggtggtg agctcgggga agtttctttc
     7861 acgtgcgttg tagcaatggg cgcagtcgtt gttgcagcga taggtgagcg ccagatccat
     7921 gcgatagggg gcggaaggcc gcgcgctaaa aggcattttg gtttccaggt caagttcatg
     7981 cactgggcag gcgccgtcag gccgaatcat ttcttcgagt tggtaattga actctgcaag
     8041 gtcttcgcgg gcctgttttt tggaaactga gtaacgggcg cggagggctt gaaccctttc
     8101 gtcttccgtt ttgccttcca ggatcaacca ggccatgaag gccgcggttg gatttaagtg
     8161 catgacactg ctggcgttga cgatgagtgt ccccgtcccg tcggggtcaa tgcgcaagtg
     8221 aatgcgcgac ttttccattt cgttttcatg gacgtagtga tagagccgtg tttcggtctc
     8281 ggctgcatcg gcctttggtg cttgctgaaa tggcagggac aggatgtttt tcagggagct
     8341 aaaattcagg gacatttttc acctcttcca tatttgtaaa gttgaatttc actttccatt
     8401 tcgagagaaa taccttgtag ctgctaaatg gataatcgtt cgggtctttt acaaggctgt
     8461 gcatgacagg gttccaaaag gtgtagcgca ggcggttgta gtaatcgtat tctgagcgaa
     8521 tgcaggtatc ccaataattc caccagacct tccttccgct tgcctgttct tcttcattcc
     8581 agtttcgcgc cgtgaagcga tggtagctgg ccacaaattt cggcaggttg tctgcagagt
     8641 cgggcgcttt gagcacggcg tggtaatgat tgtctaacac cacccaggca accacttccc
     8701 atttgtacat ttcagctgaa gttagaaagg ttttgatcca attcaacttg cgctctgttg
     8761 tttgcatgaa tggctcactg cggtaggtag aggcggtgag catgtagagc gcattcggta
     8821 caaataaatg cggaggagtg tgcggtgctt ttttgaaaat agcgccaagc atgccgaggt
     8881 ttaccgtccg ccgccagcgc aggcgcaggc acaaccagca caggcacagg cgcaggcaca
     8941 ggagcgcccg cctccgccgc tgcgaccacc gctggaagaa ctcttgggca agggattggt
     9001 cgccccagtg acgcgttcgg taaaggactg aacatttccg atcacctttt gcgagaaggt
     9061 ctgtacacct gtcaccattg atgcggcgaa atctgcaccg ggcagggctt gtccgctgga
     9121 ggaaggacgc ggggtggatg atatcgtcgg ccctgaaaca gttgtcggtg tggatatgcg
     9181 gcgataggac gggtcgtaac ggccccacca cgttggtacg tacacaggtc cattgtagac
     9241 acggcgtgcg cggtcatcgt aatctttatc aagcatggtc cattccagag attcgtccat
     9301 tttctggctc ttgacctctg gtgtgccagc cgcttccact tgagcccagg ctttctctgt
     9361 gatggatttg tagtactcga cggtttcttt gcggctaaac ccgcgcatct tttcggaaac
     9421 gcttttgata agcgcaacgg tcatatcttg cagaacgcgg ctgcgttttt catccacggc
     9481 gaatgctttg ataaagtctt tttcgtaggg atgcagggtg gcgggttgct ctgccgccac
     9541 tgaaagtttg agcgggtcgc gggtttccac ttgtgcggca ccctttttga ccacgctgaa
     9601 caggatcatg gtcatcactt tatcaagcgg ctgttccatg agaatggcag cttccaccgc
     9661 tgtgagtccg cgtttgatgc cgtgaccttc aattgccaca cgcggcggca ggtactgcat
     9721 tttgcggcgg cgccccgtga tggctgcgat ggcaggtgag ataaagaaga tgaagaagaa
     9781 aaatccaccg catagcaggc tgaaaatggt gtcttcatca atattgaaga gcggctctgg
     9841 cggcggggtg taaatggcct ccgcagggac ataaatacgt ggaaacgatg caccaaaaga
     9901 atagtagttg tacgcagtgg cagcaggtgc ataccatgtg taagtaactc ggttttccga
     9961 atcgtaggcc gcctggggtg tttcaggaaa accgctcggc gcttcgtgcc agcggggttc
    10021 ttctggattc atgttcggcg gcaggtggaa ggtcatggtg aaatcggtgt tgcctgaaac
    10081 gaattcctga ccaaaccatg tcggcgcaaa gacggcgctg gcgtagttgg cgtcgtttgc
    10141 atcgttgtat aaaacgcttg aaatattcgg gacgaagaca tgcagtatgc ccttatcccc
    10201 gggctgaatg gttcgtgacc ccatcactac tgcaaaaccc gaccctgagc cttgatagtc
    10261 ctgcgtcgtg accatcaaat ccatgccgtc agattcagcc acaatggtgg atgtattgaa
    10321 gttgtagttg ggcatgccca catctacaaa gtcaatcgga tgtgagcctg gcaggttgtt
    10381 gaagtaccag gtgtaatcca cgctcattgt gccgtcaggg ttccagtaaa cgtgaaccac
    10441 ttccttgttc acttcgaagt aatacggcgc atcctgtgca gaggcatttg aaaatgcagc
    10501 aagcaccaga atcaaaatga acgaaagcag caatagtttt cttttcatat tggcttctcc
    10561 aattgcaagg gttaccactt gggctcttcg gtcaattgat aaaccgtgcc gcagtatgga
    10621 cattccacag tgggtgcgcc cgcgatcatc ttgacattgt cggctttcaa tgcgccgccg
    10681 caactgttgc acttcatttg ctcgactttg gtttgagcag gcaggtcaat tttataggtg
    10741 atctgatccc cgcttcctgc tggacccttt tgcctgcttg caaaaacaat aagcccgatt
    10801 ccccccgcca aaaaaaacac ccccacacat gaaaatccca ggccaaatgt cagccaggaa
    10861 ctactccctt gtgccgaggt tgagccgatc acgttcaata gaccataccc caaaaggcac
    10921 agcccgatca gcagcaatat ccccgctccg atgtacaaaa aggtcttgtt cgccataaag
    10981 tcacatctcc taggttgatg tatgaaaagt ttagcatttc cgtctcattt cgnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtgatttc tatatcttcg aatccagaag
    11161 ttttcaaaag cgaggtcagc atctctgcgc tgatgacgac aatgccgcgc cctgattcct
    11221 ctgttccgcg tgaggtgagc agggtgccgc ccggacgcag cacacgcgcc agttcctgta
    11281 tcgccgcctg cttttcgccg atcaactcca atgcctccac gcaggaaacg acatcgaagg
    11341 tttcatcagg gaatgggagc ggaaaagaat cgtatttcat caattccacg cggctttgat
    11401 gcggggcaat cttttttgca gcctgtttca acatgccgtg tgaaatatcc accgcaatga
    11461 tgtggcctgt gaattcgggc tggctggcta cgacgaatgg caggcgcccc gtccctgttg
    11521 ccacgtcaag gatgaatggt tcggggacct cggtcagctt tgtgagcagc ggctttgcca
    11581 gcatttcaac atcctgcagt tgaccgtctt ttttatcgtg gtcatatttc tcggcccagc
    11641 ggtcatataa ccaggcctgc aagcgcggac cgatgtgaat gccttcgtaa cgatagattt
    11701 cccgatctaa caaaaataag agaaccagca caactgcact gccaatgatc catgtaatca
    11761 taaagactcc tgaagaattg ccgatgacga aaaccttatt cctgtatttc acgaatgggc
    11821 ggcacgaaat aaccaatgat gaaaacagct gaccctgcca gccctgccag taccagggtt
    11881 gttgataaat ggattccgtt ttgcgtgcct gtgaaacggg cgattccaag ttctgcaagc
    11941 cagcctgaac ctgccatgcc gatcaggtag ggaatttgcg taagcagctg ccgcgctgag
    12001 aagacccgcc cctgtgcgtt gatctctaca gtggattgcc acaaagccgt attgctgcct
    12061 tcgacgaatg gttcgaaaaa ggaaaagaag aaactggcga tcccccagat gagaacgctt
    12121 cgcgccagac cgaataaact gattccgatc aaacaggctg ccacaccccc aagcaggatc
    12181 ccattcacct tgcgtttagg gccgccccaa acactgagca acccgccgcc aatcaatcca
    12241 cctgctgcgc cgacggatag aatggctgcc agtgcgcctt cgctgttggc tgtgtggctg
    12301 aggatggtgg gagccagcaa agtggcaccg atggcaagaa agaaatttgc cacaacgaaa
    12361 agaatggtca atgcgcggag agtggggttg gcgaaaatag agcggaagcc gaaggaaact
    12421 tcacgggcga atcctgtttg ggtaacggga gcaggtgtga ggtcggagcg aagcgggaat
    12481 ttgatccata gcaggatgct gaatgcaaaa acaaacgtga gcaggtctat ggtcatgatt
    12541 ccgctcagtc caatactggg cagaagagcg gctgcgatca tgggtgcgaa aatagtggat
    12601 gccgcctgcg caagacccac catgccctgt gcgcgggcgt aatcgtcttt tgaaactagt
    12661 gcagttgtag cggcaacgta ggctggatat tgaaaggcgg taaagatgcc cgcaaacagg
    12721 ctgagtaaat aaatatgcca caaggcgagg ctgccagcat ggtacaagag cagggcaatc
    12781 agtgtggcgg ttacactgcc aagatcactg aggatcaggg tggttttgcg ttcccattta
    12841 tctacgaagg tgcctgcgat gggcatgagt agaatggatg gcagaaatgc cagggtgttg
    12901 atggcggcca gggatgaagc ttgttctgtt ttttgccaga cccagatggt gaatgcaaac
    12961 catgtcatgg cgctgcctag cagtgatatg atctgaccga accagatcat ggtaaatgtg
    13021 cgcattccag ttgagcgggg tggcatcata tgcgctcaat cttacaggaa aataacagga
    13081 ttttggatta cactcctatt cagatttgta aggatgccct atgtggttcg catttattat
    13141 tggtgtattc gttgtaaggg atttgcccat gttgacctct ttatgaaagg taaagccatg
    13201 aagcgagaag tggaattgca ttttgaatat gaaaatatga gcaggcgcga tgacgacctg
    13261 ttgcggcatg aacttgaaga acgcagaaag tctctgctgg ataaggtcgt gacggtgcag
    13321 gattccagtt tcagccttga agaacatgaa tggctggcgc gctccttccc tgtgctttca
    13381 atccttgcgc ctgtgatgtc tacaaatgag gggaagatcg aatttcctgg cgacccgatg
    13441 tgtttatatt ccgcattgag ctatgcggtg gatcaggccg tgaagacccg caagttgggg
    13501 ctttcggagg gtgcgccgta taacgacttg tgcccgcagt ggggggcgtt gccctcggca
    13561 gattatcgcg ctgctgtgga tgtgaatggc atccgtgatt atcacggcgt tttgctaaac
    13621 accgatcaaa ccgtgttcga cccgcgtgtt tggaatggat tggtgaagaa gtattttgta
    13681 gaaaaggttt tgctggtgat gcgcccaaag gtggtgttga tcagtgcggt ttctcctgct
    13741 caccgttatg cgatcgatat tgcccgtgtt gtgcgtgaac acctgcccga ttgcctgatc
    13801 gttcttggcg gccgccatgt ggatgaaacc atccattttg acctggtcaa tcggaaggtc
    13861 gtatttgaac caagcagtgc ggttgtgaaa attcaagaag ggacggttga acctgttttt
    13921 gattttctga tcggggggga tggctattat gccctggatg ttttgatgaa ggcgatctcc
    13981 atttcgatgg accttgagac aaagtctaca cagattgatg cggtgatgaa gaccttgtcg
    14041 accattgcgc ctttgttcga tccgataccg ggcaattctg tgatcgtggg aatggataag
    14101 agaaaatctg tccatgcctg ggtgataaat tcacgcatca agctggacct ttcggttttg
    14161 ccttcgccct atcaggcttt tgccgttcga gctttgttcc ccatttttca acgcgacggg
    14221 cgggtgttgc ggaccgcaca cttcatggtg accaattcat gcacatatca ttgttatttt
    14281 tgctccgaag gtgtgacggt ggtgggaact ttccgttcgt tcaattcgga cggcattcag
    14341 cgtgcattgg agaaagtggt ggaatacatc tcttatggtg ccgaggcgtt gtttttcgat
    14401 gattcaattt tttggggcgg caattttggg aatgtcattc atttttgcag ggagtggctc
    14461 cacttgcgtg aattggcaaa aaaatccccc tcgaagatga tcaaactttt tgggtatgag
    14521 gtggaggcgc aaaaagtatt ggatttggtg tggggagcgc aatttactgt ggatttgctg
    14581 gcgtcccgtc gcattcctga ggaggccatg ctggcgctgc atgaaatgaa atcggcgggt
    14641 tgtgtttata tttatatggg gatcgaaagc atgtctgcgc ctgttattga caaggtgcat
    14701 aagaatgtca accgcaaaca ggcatgggat gaacgcgtcc gtgccgcgct ggggatggca
    14761 cggttggcgg ggatcacagt cggctcttcg gtgttgttcg gtttggacgg cgagaccatg
    14821 gagaccatcg aggagaccat ttccaaagtg gaagagctgc tggtggaaga tatcctctcg
    14881 atcgccagtc caaacatttt gacctaccac cccaatacgg caataaccgc tcaacatggc
    14941 atgaaggata agatcgatta tcactccatt ggcattgaaa accgcccgcc ctatgtatat
    15001 tttgaagagg ccttcccttc ggtggtttcg cggaatctct ctgaagagca gatctggttc
    15061 attcacgagc aggcaacccg caggtggggt gtgaagcgga atcttaatcc gatgccagaa
    15121 gtgattctac gtgaaggggg gtaggtcagt tttgaggttt caggcgggcg gtcaggtcac
    15181 gtagttggga aaatgtgatc ggtttgatca gtacgaggtc tgcctggccg cgtgagtttt
    15241 cacccatttg tgcatcggca gtggtgacca caacccgtgt atttgtcagg cggtcatctt
    15301 cacggatctg gtttaacaga atgtctcctg aaatgtgagg aatgtgcatg tccaatgtga
    15361 tgacggttgg ggtgatctct ttgagacgtt cctgcgcaat ggcgccatca cgaatgattt
    15421 cgatggtgta tccggcagct accagtgcct gcccgaaaat ttcagataag tcttcgtcgt
    15481 cttcgatgat cagtgcaaga tgttcattca tggaaactct ccttattctt tttccagcaa
    15541 tgtttcggct tcaatgggga cgatgagggg cagggtaacg gtaaatatgc tgccgcctcc
    15601 aatttcactt tctacattga ttttgccctt cattaactca accagttgtt tgacaatgga
    15661 aagtcccagc ccaacgccgc cgtgttgacg tgtggctgat tctttcacct gacggaagga
    15721 ttcaaaaata tattccttgg cttcttctgg gatcccttca cctgtgtctg aggtttggat
    15781 ttgccagttt tgatcgtcca gtcgcgaaag tttaacgcgg atgctgcctt tctcagtgaa
    15841 cttgaccgca ttgtttgaga ggttgaccat gatctgctgc agacgtttgg ggtctccctt
    15901 aagaacaggc ggcatggatg ggtccaattc ggtaatgaat tgcagtttct tgtcggatgt
    15961 gatcttatcc attacgctgt gcaggttttc cagcagatct gatggtttac atgcgatcat
    16021 ttgtatcttg agtttgcccg cttcgatctg aacttcgtcc aacaagtcgc tgacgatatc
    16081 aagcaggcgg cgggtgttgg tcatgatgcg tagcgagatg ttttgctgtt tttcattggc
    16141 ggggccgtat acgccttctt ttagcatttc tgcgtagcct aaaatggcat tgagcggagt
    16201 gcgcaattcg tgtgaaacga tgccgatgaa agtgtttttc atcttttcga gttccacctc
    16261 atgagtcaca tctcggaaca cggccacggt gccgatgttt ttgccgtcag aactgtgcac
    16321 ctgggcgaaa cttgtggaaa gggtcttgtt gccccagtcc atgcggatgc ctgtagcgga
    16381 tgcctgtggg ttttctagca ggccgagcag ggttccgcgg gtggtgggtg aaatcaaatc
    16441 gttttggatg aagtcggtga ttttgcggtg gagcaaaccg tccttgggta gatcggccag
    16501 cctgctaagg gcaggattcg ccagaatggc ggtattgaat tcgtcgaaca cgatcacgcc
    16561 atcggcaatt gaatcaagaa tggcctggtt gcgccccgcc aaaatcaatt cacgggagag
    16621 tgtgctggcg agttccctgg ttttttcctc tacaacttta tccagattga gattgatgga
    16681 gcgtaattca gacagggctt gttcgaggtt tcttgaggaa agccatgcga tcaaggccac
    16741 tagaaaatat cctgcaatgg caggtgagtt tgggtcttcc tgtattgaga tggcgagggc
    16801 tgagatgatc acactgctga tggttgcgaa caagaagctg gagactggtg tcaacaatac
    16861 gcttgccata atgatgggaa ttgtaaaaat gaaaaggctg cgcccattgg caatttcatg
    16921 cggactgtcg ctgaagatca tgacgcttgt gatcaggaaa aggaacagga tggctgcaat
    16981 ctttcctggt aacgatctgt tcaggaggta aatgatcccc atgcctagta acagggctat
    17041 ccccccataa acgaccagca gggttccttc ctgagcattt tggctcaaga gggagattac
    17101 catgacgatg atcaggatga tggtgaggat gcccagtcca aacagcaaaa tattgagcag
    17161 tttttttctg cgggcgtcat ctgggtcatt ggctgagatg gcaagaagtc tatcgaacat
    17221 tttcgcgcct tttctggtga tcgatttaat ttaaagcgtc aacgcggtca tgatacagga
    17281 ctgaataatc agttctggat aatataacgt ctttgcaatc cacatccacg gatatggagg
    17341 tttggagttt tctgaaagcc acatgtgggc catttcaatg cgtccgctgg gtactgcatt
    17401 gccgctgttt cgccacacct tgagtgcttg taaacaaaaa gcggtctctt cggcagtgga
    17461 gataccaaaa tatccccatg cgccattggc attttgagtg tttagaatcc aatctactgc
    17521 ttgttgacag agtttcaggt cgtattcaag gcaatcaatg atgacatgtg aagtgatgta
    17581 ataaggcgag acatgccact tatcaaacca atagcctttg tcggtgcggg tgttgtacaa
    17641 aaatttcagg atcttttgaa cgctgggatg ctttttttcg aaccctgccg cctttagtgc
    17701 gccgaggatg tgaatgttca cgccgacgga tggactggcc tcatgctgta cgcagcgaaa
    17761 atattcctcc tcttcataac tcaaaacggc ttcaatgtcg gcttcacggc caaagcgcag
    17821 taaaacccgc tgcatgacac tggtgtcgtc actatcgtac atggcacaat cagaagcaaa
    17881 cccgatgcct gttttgggca cccagcgttt tgatatgtag tcgagatgcc cctgaataag
    17941 atctgtttcc tgcggagtgt ggctttccag aagcgaaaga ttccaaagta cccaacagcg
    18001 ttcgaaaata tcgaaggggg caaaattcgg tacgccgcca tcttttgaaa tgttgcgaag
    18061 ataggagagt gctttctcgt ccccgggttt caggtaccga gcaaaatatg ccgtggaaga
    18121 tgggctgcaa cccacggagc cattcggctc ttgtaaatga tcaaagtcaa gcaggtcggt
    18181 tgaatcggtc cctgccattt ctgctgaaaa gactgccgtg atattgcggt tgattttcag
    18241 ccc
//
LOCUS       DAOQJC010000083        18041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1454_length_18041_cov_9.047704, whole genome shotgun sequence.
ACCESSION   DAOQJC010000083 DAOQJC010000000
VERSION     DAOQJC010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18041
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1454_length_18041_cov_9.047704"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PLE39_12790"
     CDS             <1..543
                     /locus_tag="PLE39_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69252.1"
                     /translation="KETAMIFGKKQEKSIRNIRVSILTNEYLVEGLDDSDSSALSASF
                     DAEVEDPAGGCLILKDTHVRSMGNIHTPARTFPEWHIPSLTNVVAMFSDDPAAEDILL
                     EVWEDHQTPHKVIIYSGVYVIEGTIYSEEEAPPDFMLHAFAPIENLKITNQVDKKAEP
                     IHAKWGVVNSTMMQGYGVEK"
     gene            759..4802
                     /locus_tag="PLE39_12795"
     CDS             759..4802
                     /locus_tag="PLE39_12795"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNE69253.1"
                     /translation="MFARKLFAGVLLAGLVFSQLAVSSAAAKACNQAQFISDLTVPDG
                     TSYTVGTTFTKTWRLLNIGSCTWTTSYSLVFVKGDQLGAPASTNLPVEVAPGQMVDLS
                     INLAAPQTSGHYRGYWALNDPSITAGAFGIGTSGTDPFWVDINVLGTSAVIYDFVANA
                     PYAEWKSGSGILPYPGASGDNRGYSQLIDFPRLENDSIDSQPGLLTVPQNKYNGFIQA
                     TYPEFLVSKGDRLQTLVNCEYGATGCYVTFGIDYVTSTGSVKGLWSWKEAHDGRFYRA
                     DIDLSILAGKKVKFILKLLSVGSASGDRAVWGSPRIVRSSVETPPAPPSTLTPLPPLT
                     PTLTPFATAPPISAAGCNKAAFVADITVPDGTLFSPGAAFTKTWRIKNSGSCGWTTAY
                     SLTYYSGERMNAPTSLPLPKAISPGATVDLTLNMTAPASPGKYRGYWILKNAAGELFG
                     IGTNASMPIWIEINVTGSPALNTGYDFAANACSAEWKSGAGILPCPGTTTDKNGFILK
                     VDSSQLEDGTTNSNPALLVGPQNKYNGYVQGIYPTMTVQPGDHFRTTVGCEFGTSCYV
                     AFKLDYMTSTGTITNFWTWREQNEGRPNNIDLDLTPLVGRSVRFILTLSANGSATNDR
                     ALWSAPVIIRTNLTPTFTPTSSPTPQVNDWLTYSNSDHGFQFKYPKSSQINEMLENSI
                     SMNLPIAAGTNLSQKSLQVIAVQNANPCSSYLGRSSMLSSSETVLINGLTFLKETGGD
                     SAAGQLHQWVAYSTLRGDTCVTFDMILHIINLGNYLTPPPAFNETAEISVLKDVVSTF
                     TWLGATPTPTFTPTPFGYDWPVYFNSKYGFQIQYPKEATLAIQNDNAAHLNLPFAAGT
                     NLKEKIFDIFIREDLQTCRSIIASSSMLVTSETVTINNTTFLKEVGEDGAAGSLYQWV
                     AYSTLKNNACISFEMRLHSISPGVYETPPPIFDYAAETSIIQNMLATFGWSPATSTPT
                     PTAIPTSAATLSAIFEAMNARDYGTLKASMGETFAIAYWQSQGTSSTPDEAIEQLKTY
                     YLASPKALTSDPNRDLTTLLGGANPYTLMGLDPTKSQALLVSGLGPQGIDEAILYMTQ
                     RADGTPYWFGLLTAKGGFVSFTPYAVTLIATGGVLDVYSAPGISNPIVASFPFDGINI
                     KRTGPTQLEAGTEWVEVIRPDGGTGWVNSHYLTEYIPRDGFCADARIAPIIAQLKNSV
                     TLSDGTLLGSLTSPKHGLNVNYWQYSNTVNYTSAAIQTIYTDPQVIDWGSGGASGITD
                     IGTFAQIVQPQLADVLNSSYELHCNEPSYASMYPTPWRYVNINYYAVVKPPTPETVFD
                     WKVWLVGFEYINGQPYLFGVTHFVWEP"
     gene            4896..5312
                     /locus_tag="PLE39_12800"
     CDS             4896..5312
                     /locus_tag="PLE39_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNE69254.1"
                     /translation="MELPIFLGSGLFTVAILLCTCLITLVSLGAMGFFFYRMFKNSSQ
                     NNAVLQSGIQAPAVILNVEDTGASMNDNPQVRFTLQVNPPDGRPSFQAVTTKFISRLQ
                     IGMINPGSPVTVRYDPNDTTKVAIETLGAPFVPPQK"
     gene            5385..5642
                     /locus_tag="PLE39_12805"
     CDS             5385..5642
                     /locus_tag="PLE39_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69255.1"
                     /translation="MAKLIPASERIIRAKKFIQEARELPIPAEGGRYNFSYVAGVRDL
                     LRQARDMVKFISMTPSATADMKAEVKKVIEEIERANTEILG"
     gene            5720..5974
                     /locus_tag="PLE39_12810"
     CDS             5720..5974
                     /locus_tag="PLE39_12810"
                     /inference="COORDINATES: protein motif:HMM:NF025715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HNE69256.1"
                     /translation="MENKELEKLLEKLHAELEKVDHMDEDGMKLLRGIEKDINELLHQ
                     TDRDTLIGRLREAIEKFEVSHPTLTTMLSEISSILNIAGI"
     gene            5984..6352
                     /locus_tag="PLE39_12815"
     CDS             5984..6352
                     /locus_tag="PLE39_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69257.1"
                     /translation="MEKKPSFLWALAIGAAFPILQVLVFLIRFNDLAVGASFADYIFF
                     FVGGLLIGLALIAALRRCETRGAYRATLIGFGLGLPFAVFGMVVGGMLGPVGVIMFSV
                     SPGVFFILVGYLIGRATFQV"
     gene            complement(6407..7261)
                     /locus_tag="PLE39_12820"
     CDS             complement(6407..7261)
                     /locus_tag="PLE39_12820"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE69258.1"
                     /translation="MEMIDQITTHSGVLQSGQFVNYVQQGRGAPVILTHGLAASLHDW
                     DDLLPELAASGYAGFALDLLGHGESYKPVNHQDYSFDHVYDHYSAWVNSLNQTEPMIL
                     IGHSLGGGLSLQYTLQHPERVRALVLVNPFYDIHQLPPVLRLAFRRKLINTSLIDRTP
                     YRLFRLMVDLTSFNYYIGHRETHVLPEHIRYQTALDYKRASSGIYNIPRSFPNLTSSL
                     PQIKQPTLLLWGGRDQTLAPQSFPRIGEFLPNVVASHALPICGHVPHQCHPEKFNPHV
                     MKFLKDLA"
     gene            complement(7342..9813)
                     /locus_tag="PLE39_12825"
     CDS             complement(7342..9813)
                     /locus_tag="PLE39_12825"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE69259.1"
                     /translation="MDIDFDLYRHEIRISSDPLVRLSAIDVSPELPKRTLVFIHGFGG
                     KAEQWQYQMQKFALDNRVIALDLRGHGLSDKPTSGYDMPRIQLDLETALTQLKVSTPF
                     VLIGHSFGGAVVTEYALNHPDHIEKLIIIATAGEFKLRPLFKFALNLPASILRLIGPF
                     TRKWLHAPPHALRPFYLENLSKWVGWERFAKLQVPTMIVRGHRDIVFDRPLFEKVAGS
                     IPGGEDVDIGVSGHMVMLERREAVDRAIARFLKGETKKSWRDDASLVVSKSTRDELKS
                     QRVWLNHYENGVPYTVDVPRIPAHHLLRSSVRRFPNHAAIFFEGAKLTYRKLNHEANR
                     FANALLAQGIGKGARVVLLLPNVPQMVIGFYGALKAGAAVVLIPPMIEPEELTRQIKE
                     ADASVLVTLSMWSGLAKQIQRDAGTPHVVLTDPAEYLPLPKYLISRWRNRGLDVQNAL
                     HWNRWLSAQSDKSPAVEVQPEDLAAIIFTGGTTAQSKGVMLSHRNLVANALQTRHWLP
                     EAMEGRERFLCVVPFFHSYGMTAALNVPVSLGAAMILKAQFQALEVLKTIKKYKPTIF
                     PGTPNMYVAITNFRGVRKYGIGSIKACISGSAPLHVEVQESFEKLTKGKLVEGFGLTE
                     ASPITHANPLGKNRKVGSIGVPLPSTHAAVVDLKSGRKEVEPGQIGELAVRGPQVMMG
                     YWKNEAATREVLTDDGWLLTGDVAQMDEEGYFRIVARKADMWYPEKPGAPAFPRDVEE
                     VIYEIPQVKETVVVAVAGHPFAFVIAGKEKPSAESVIAYCKRRLPPHLVPRFVIFMDD
                     FPRTFIGKVLRRELAKRYGKQIAGE"
     gene            10183..14637
                     /locus_tag="PLE39_12830"
     CDS             10183..14637
                     /locus_tag="PLE39_12830"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE69260.1"
                     /translation="MGIYLVSRDLKKQFREREDILLSVRKSHELLLSSEEKFSKVFQL
                     SPAIIALTMLEDGTFLDINQKFLDISGFTRAEVIGKTSMEIGWVSPEVRDALIKEIQI
                     AGRVTNREVILHAKNGEEILCIYSGELLELGDKTYVLSLAQDITERRKAEEALQSSEA
                     QYRLITENMKDMVWLMDMEFKPIYISPSVTKLRGFTLNELQVLPLEKNLAPDSMRRAL
                     EVMNVSLAPEMIASTHSYKIELEYYKKDGSTYWSEDTFVLIRSEEGHPVGILGGGHEI
                     TEKKKAEDALRESEEILRTVFNTMEEGLALNELVYDENGEIVDYRILEVNPAYERITN
                     VPRDQAIGKLATEFYEMPTEYINAFWKENIDNDHIVTELYEDRLKAWKHISTSRPRDG
                     KFTTIFFDITEQKKSEEALRTSEQQYRLLFEEMMDGFALHEIICDENNIPIDYRFLAV
                     NPVFEKLTKLKAEDVIGKTVLEVMPDIEDVWIKRYGQVALTGEPAHFENFSTTLQTHF
                     EVRAFSPARGKFAVIFHDVTQQRNAQMIMLARMKFIESSTVQPLDRLLQTILDEAEDM
                     TGSKVGFYHFVGADEQTLLLQSWSTRTASEFCKADGKGLHYPVERAGVWADCVREKRP
                     VIHNDYKSLPHRKGLPEGHAELIRELVVPVLRRGKVVALLGVGNKEFNYTEQDVAIIG
                     QLADLVWDIVESKRVEEIQKRVEQDYEALIQSIDGIVWEADVHTLEFSFVSQQAERLL
                     GYPIERWTTEPGFWIDHIHPDDRAASISFCVAQTRLKKSHEFEYRMFTADDRIIWLRD
                     VVSVVLENDHPVRLRGIMIDITEKKMADDRLRQLSLVVEQSPASVVITDLNGNIEYVN
                     QKFMQVSGYTLDEVLYKNPNIVQSGKTPKETYAQLWKNITSGKVWHGEFQNKRKNGEY
                     YWEDVLISPLLDTNGKITHFVSVKEDITERKQAENEIIEKSQELEALFKISSHLRTAQ
                     TVPEMIPVVLKQMRETFNSDANAVILINRDHTQFKYVLGDGLLAENTGLQFGIEHSIS
                     RRVMDTLEPYITLDFTSDLIGASAVKVIENIGATVVVPLQSETEFLGTLLCARAKDSQ
                     LAPFTSAEIKLLRAIGEMVGNALRRAKLYDDALSRLQRVEALRSIDMAINANMDALLT
                     LNMLLSQTIALLKVDAAAAMLYNPASHMLEYGAGQGFHHKEIEKANQKLNRGFANTAI
                     IERRLVTARNLKLTEDQSYRAIAIKEGFETCHIVPMIANGQVCGILEVYNKTEISPDQ
                     EWLDFLEALGGQAAIAIHSAQLFENLEKSNLELSLAYDATIEGWSKALELRDKETEGH
                     TLRVTEWTVKLAMLAGMGEADIVQVRRGALLHDIGKMGIPDHILNKNGELTSQEWEIM
                     RQHPQYAYNMIHPISFLRPAIEIPYYHHEKWDGGGYPFGLKGEQIPYSARLFAIIDVW
                     DAITSNRPYRKAWSFEKALEYIKSESGRHFDPNIVELFLRMVNGFRNE"
     gene            14707..15036
                     /locus_tag="PLE39_12835"
     CDS             14707..15036
                     /locus_tag="PLE39_12835"
                     /inference="COORDINATES: protein motif:HMM:NF020908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HNE69261.1"
                     /translation="MSLEKAVEAIIKEAQERGDFDNLKGKGKPIDLSAYFETPEELRN
                     AHAMLKSAGMVSAEVDLLQEIAALKERLAATYEEKDRSRIKKIIRDKELQFNVMLERQ
                     KRQRKRK"
     gene            15341..16984
                     /locus_tag="PLE39_12840"
     CDS             15341..16984
                     /locus_tag="PLE39_12840"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNE69262.1"
                     /translation="MSKKNKLRIIPLGGLGEVGKNMMAYEFGGDIMVVDAGIMFPHND
                     MLGVDYIIPDFEYLMKRADRVRGLVITHGHEDHIGAIQHVIDQINVPIYATPLTRGLI
                     EGKLSRNNAQHKAQFKTIQAGESSNIGPFKVEYFHVSHSIPDAVGLGITTPAGLVVHM
                     SDFKFDHTPVDGWPTDFAKLSEFSTRGVDLLLSDSTNAERPGWTPSESVIGPAFDKVF
                     AEAKGRVIVATFASLISRVQQVADAAAKHGRKMALAGPSMVDNVKIARKMKYLNIPDE
                     MIVPIDQALNMQDHKVAIMCTGSQGEPSSIVGRLSAGTNRQFDLKPGDTIVLSSHPIP
                     GNEETISKTINRLLRRGARVIHDGIAPIHVSGHAAQEEQKLLINLVRPKHFMPIHGEL
                     RQLMRHAELAQQLGIEPENTIVVENGQVVELVGNKILLGERIPGDYVFVTGESIGDID
                     HDVMRERSQLARNGIMLIDISLDKLTGKLLHDPEIITRGFVSPEDAEILIPEVQKRVM
                     DVVHSGGLDDEKDIVNAVKSYLHQETKRRPMVFVTLSKA"
     gene            complement(17041..17907)
                     /locus_tag="PLE39_12845"
     CDS             complement(17041..17907)
                     /locus_tag="PLE39_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69263.1"
                     /translation="MSPSSKKSQTKKSFWDRLVQFIPVWVQIVICIGTIAVAAVPFVF
                     PRILSEETPTPTSALPTDVLPPAVSQAEEPTETPSPIPAPATPELVAPVLPPPVPGED
                     WARNCIDTTIWSTFLADKITRGSTPCYELTQWGITGESGKVIFVTHKSQSRAVEYGIF
                     TPWQDWKGVEFSVHKSHLENSEVWVGFFEDETVYSNGIVFVIQPDDVIDIRQLPVEDL
                     PVNNVGLPFADGVFNVDIVLREGKFKASVDGQGIISEWPLSFAIKYMFVGYRSLPSTN
                     LDASVSDLKFIK"
BASE COUNT         4700 a         4760 c         4416 g         4165 t
ORIGIN      
        1 aaggagacag ccatgatttt cggtaaaaaa caagaaaaaa gcataagaaa catccgcgtt
       61 tccatcctga ccaatgaata ccttgtggaa ggtcttgatg actccgattc atcggcactc
      121 tctgcctcct tcgatgcgga agtggaagac cccgcaggcg ggtgcctgat attgaaagat
      181 acccatgttc gatccatggg aaacatacac acacctgcgc gcacctttcc cgaatggcat
      241 atcccctcgt tgacaaacgt cgtcgccatg ttctcagatg accccgcagc cgaagatatt
      301 ttgctcgaag tatgggaaga tcatcaaaca ccccacaagg tcattattta ttcgggcgtg
      361 tatgtcattg aaggcaccat ctactcggaa gaagaagccc cgcccgattt tatgctccac
      421 gccttcgcgc ccatcgaaaa cctgaagatc accaatcagg tagataaaaa agcagaaccc
      481 atccacgcca aatggggcgt agtcaattcg accatgatgc agggctatgg ggttgaaaaa
      541 taaactgtct gtttttttaa ccaagcccgc agaccgtaga ccacggtctg cgggtaaatc
      601 tttcactggg ggccaacaat tcaaaccatc cattttcctg tcatgctatt tcaacactcc
      661 aacaagttga ttcgtgtcat catgaaagat gaagacttga acagacgatt tgtcaggtct
      721 gttcggccat ttagccgtgt gttcggctgg aggtcaagat gtttgcacga aaactttttg
      781 caggggtgct attagcgggt ctcgtttttt cccaacttgc ggtttcatcg gcggcagcaa
      841 aagcctgtaa tcaggctcaa ttcatctccg acctgaccgt gcctgacggc acgtcctata
      901 ccgtgggaac caccttcacc aaaacctggc ggttgctgaa cattggctcc tgcacatgga
      961 caacctccta cagtctggtc tttgtcaaag gtgaccaact gggtgcgcct gcctcaacaa
     1021 atctacccgt tgaggttgcc cccggtcaaa tggtggatct ctccattaac ttggccgctc
     1081 cacaaacaag cggtcactat cgtgggtatt gggccttgaa cgaccccagc atcaccgctg
     1141 gagcatttgg aatcggcacc agcggcacag acccattctg ggtggatatc aatgttttgg
     1201 gaaccagcgc cgtcatttat gattttgttg ccaacgcacc ctacgccgaa tggaaaagcg
     1261 gatcgggaat cctgccctac ccaggtgcca gcggtgataa ccgtggatac tcccaactga
     1321 tcgattttcc ccgccttgaa aacgattcga tcgattctca gcccgggctg ctcaccgtac
     1381 cccaaaacaa atacaacgga ttcattcaag ccacctatcc tgaattcctc gtcagcaagg
     1441 gtgatcgcct gcaaacgctg gtcaattgcg aatatggagc cacaggctgt tacgtcacct
     1501 ttggcattga ttacgtcacc tccacgggca gtgtgaaggg attatggagt tggaaggaag
     1561 cccatgatgg ccgcttctat cgtgccgaca ttgacctgag tatccttgcg gggaagaagg
     1621 tcaaattcat tctcaaactg ctttcggtag gatctgccag tggtgaccgg gcagtttggg
     1681 ggtcgccacg aatcgtacgt tccagcgtgg aaactccacc ggcgccgcca tccacactga
     1741 caccactgcc gccattgacc ccgaccctga caccatttgc cacagcgccg ccgatctctg
     1801 cggcaggttg taacaaagcc gccttcgttg cagatatcac cgtgcccgat ggaaccctct
     1861 tttcaccagg cgcagccttc acaaaaacat ggcgaatcaa aaacagcggc tcctgcgggt
     1921 ggaccaccgc ctacagcctg acgtattaca gcggcgagcg gatgaacgcg ccgacctcac
     1981 tgcccctacc caaagccatc tctcccggtg cgaccgttga cctgactctc aatatgacgg
     2041 cacccgcttc acctggaaaa tatcgcgggt actggattct gaaaaatgcg gcgggggaac
     2101 tattcggaat cggcaccaac gcttccatgc caatctggat cgagatcaat gtgacgggtt
     2161 cgcccgccct caacacaggc tacgattttg ccgccaatgc ctgctcggca gaatggaaga
     2221 gcggtgcggg tatcctcccc tgcccgggca caaccactga taaaaacggc ttcatcctca
     2281 aagtggattc gtctcaactc gaagatggga ccacgaattc caaccccgct ttgctggtcg
     2341 gcccgcaaaa caaatacaac ggctatgtgc agggaattta ccccaccatg accgtccaac
     2401 ccggggatca tttccgcacg acggtgggtt gtgaatttgg caccagttgt tacgttgcct
     2461 tcaaattaga ttacatgacc tccacaggca cgatcaccaa tttttggaca tggcgcgaac
     2521 aaaacgaagg ccgccccaac aacattgacc tcgacctcac gccgctggtc gggcgcagtg
     2581 tgcgctttat cctcaccctg tcggccaacg gctctgcaac caatgaccgc gcactctggt
     2641 cggcacctgt cattatccgc acgaatctca cgcccacatt tacaccaacc tccagcccca
     2701 cacctcaagt caatgactgg ttgacctact ccaattcaga tcatggattc caattcaaat
     2761 atccaaaatc gtcgcagatc aatgaaatgt tggagaattc gatcagcatg aatttgccca
     2821 ttgcggcggg cacaaacctc agccagaaat cactgcaggt cattgcggtg caaaatgcaa
     2881 atccgtgcag cagttatctt ggcaggtcgt ccatgttgag cagctctgaa actgtgctga
     2941 tcaacggcct caccttcctc aaggaaacag gcggcgacag cgcggcagga caactccacc
     3001 aatgggtggc ctactccact ttacgcggtg acacctgcgt caccttcgat atgatcctgc
     3061 acatcatcaa cctgggtaat tacctcacac caccacctgc attcaacgaa acagccgaaa
     3121 tttccgtatt gaaggatgtg gtctctactt tcacatggct gggagccacc cccacaccca
     3181 ctttcacccc aactcctttc ggctacgatt ggcccgttta tttcaattca aaatacggct
     3241 tccagatcca atacccaaag gaagcaacac tggccattca aaatgacaac gccgctcatc
     3301 tcaatctgcc atttgccgca ggcaccaact tgaaggaaaa gattttcgat atcttcattc
     3361 gtgaagacct gcaaacctgt cgcagcatca ttgcatcctc gtccatgctg gtgacctctg
     3421 aaactgtcac gatcaacaac accaccttct tgaaagaagt cggtgaagat ggcgcagcag
     3481 gcagtttata tcaatgggtt gcctattcca cgctcaaaaa taatgcctgc atcagtttcg
     3541 aaatgaggct gcattccatt tctcctggtg tgtacgaaac gccacccccg atctttgatt
     3601 atgccgcaga gacctccatc attcaaaata tgctcgccac ctttggctgg tctcctgcca
     3661 catccacacc aaccccaact gcaattccca catctgccgc cacactcagc gccatttttg
     3721 aagccatgaa cgcgcgagat tacggcacac tgaaagcctc aatgggtgaa acttttgcaa
     3781 tcgcatattg gcaatcgcag ggaacctcat ccacgccaga tgaagccatc gaacaactga
     3841 agacctatta tcttgcctca cccaaagcac tcacctccga ccccaatcgc gacctgacaa
     3901 ccctgctcgg tggagcaaat ccatacacac tcatggggct cgacccaacc aagtcacaag
     3961 cgctgctggt ctcagggctg gggccgcagg gtattgacga agccattctc tacatgaccc
     4021 aacgagccga tggcacgccc tactggtttg ggcttctcac agccaaaggc ggtttcgtct
     4081 ccttcacgcc atatgcagtc acattgatcg caacaggtgg agtgcttgat gtttattccg
     4141 ctccgggcat ttccaacccg atcgttgcct ccttcccctt tgatggaatc aacatcaagc
     4201 gcacgggtcc cactcaactg gaagctggta ctgaatgggt tgaagtgatt cgccccgacg
     4261 gagggaccgg gtgggtcaat tcccattacc tcacggaata cattccccgc gatggcttct
     4321 gcgcggatgc ccgcattgca ccgatcattg cccagctaaa gaactccgtc actctttcag
     4381 atggtaccct gcttggttca ttgaccagcc ccaaacatgg gctgaacgta aattactggc
     4441 aatacagcaa cacggtcaac tacacttcgg ctgccattca aacgatctat accgatccac
     4501 aggtgattga ttggggcagc ggcggcgcca gcggcatcac cgacattggc acattcgcac
     4561 aaatcgtcca gccgcaatta gccgatgtgc tcaactcaag ctatgaattg cactgcaatg
     4621 aaccatcgta tgcctcgatg tacccaaccc cgtggcggta tgtcaacatc aattattatg
     4681 cagtggtaaa gccgcccacc cctgaaaccg tcttcgattg gaaggtatgg ctggtgggct
     4741 ttgaatacat caacggccag ccgtatctat ttggcgtgac ccattttgtt tgggaaccat
     4801 aacatcatgc tcccccacct ttgggggagc atgcgtaaaa tacacggtga tataatgccg
     4861 ccaactcaac caacttgaaa taggagtatc aactcatgga attgcccatt tttcttggct
     4921 ctggcctgtt caccgttgcg atccttttat gcacctgctt gatcaccctg gtttcattgg
     4981 gagcgatggg ctttttcttc taccgcatgt tcaagaattc aagccagaac aatgccgtcc
     5041 ttcaatcggg tattcaggct cctgctgtaa ttctcaatgt ggaagatacc ggtgcctcca
     5101 tgaatgacaa tccacaggtt cgtttcacac tgcaggtcaa ccctccggat gggcgtcctt
     5161 cctttcaggc ggtgaccact aaattcataa gccgcctgca gatcggcatg atcaaccctg
     5221 gctcccctgt cacagtccgt tacgacccga acgatacgac caaggtcgcc attgaaacac
     5281 tgggtgcgcc tttcgtccct ccgcaaaagt agacttcatc atcccagccc accccggacg
     5341 cggcaatcgc tgcgtccttt ttattacaaa acaaaggagc aaacatggca aaactcattc
     5401 ctgcaagcga gcgcatcatc cgcgccaaaa aattcattca agaggcgcgc gaactcccca
     5461 tcccagccga aggcggacga tacaatttct cttacgtggc gggcgtgcga gacctgctgc
     5521 gtcaagcccg tgacatggtc aaattcattt ccatgacccc ctccgccacc gcggatatga
     5581 aagccgaggt aaagaaggtc attgaagaaa tcgaacgcgc caacacagag atattgggat
     5641 gacatattca cccggaacgt tgtaaaattg aaatagaggc aggtgcgggt tcacaaatcc
     5701 aaatgacgag aggtgcatga tggaaaacaa agaacttgag aaactgcttg aaaagctcca
     5761 tgccgaactg gaaaaagtgg atcatatgga tgaagacggt atgaagctcc tgcgtgggat
     5821 cgaaaaggac atcaacgaac tactccatca aaccgaccgc gataccctga ttgggcgtct
     5881 gcgggaggcc attgagaaat ttgaagtgtc gcatcccaca ctcaccacca tgctttctga
     5941 aatctcctcc attttgaaca ttgctggcat ctagggaata attatggaaa aaaagccatc
     6001 ttttctatgg gcactcgcaa ttggggcggc tttccccatt ttgcaggtgt tggtgtttct
     6061 catccgtttc aatgaccttg ccgtcggggc atccttcgca gattatattt tcttcttcgt
     6121 cggcggcctg ttgatcgggc tggcattgat cgccgctctg cgccgttgtg aaacacgggg
     6181 tgcataccgc gccacgctca tcgggtttgg cttggggctg cccttcgccg tattcggtat
     6241 ggtggtggga ggcatgctcg ggccagtggg tgtgatcatg ttcagtgtat cccctggcgt
     6301 gttctttatt ctggtcggct atttaattgg gcgggctacg ttccaggtgt agcaggttga
     6361 taaaatttga ttttgaaaaa tcccgctccg atgagcggga ttttttctat gcgaggtctt
     6421 tcaaaaattt catcacatgc ggattgaatt tttcagggtg gcattgatgc ggcacatgcc
     6481 cgcaaatggg cagggcgtgt gaagcaacca cattggggag aaactcacca atgcgcggaa
     6541 aagattgggg tgccagggtt tggtcacgtc caccccacag gaggagagtc ggctgtttga
     6601 tctgtggcag gcttgaggtc aggtttggaa acgagcgcgg aatgttgtag attccagaag
     6661 aggcgcgttt gtaatccagc gcggtttgat atcgaatatg ttcaggcagg acgtgtgttt
     6721 cgcgatggcc gatgtagtag ttgaaactgg tcaaatccac catgagacgg aaaaggcgat
     6781 agggggtgcg gtcgatcaag ctggtgttga taagtttgcg tcgaaacgca agccgcaaaa
     6841 caggcggcaa ctgatgaata tcataaaacg gattgaccag caccaatgca cggacgcgtt
     6901 caggatgttg gagcgtgtat tgcaaagaga ggccgccgcc caacgaatgc ccgatcaaga
     6961 tcatgggctc ggtctggttg agcgagttga cccaggcgga ataatggtca taaacatgat
     7021 caaacgagta atcttgatga ttgactggct tgtaactttc gccgtgcccc agcaggtcaa
     7081 gtgcgaagcc cgcgtaaccg gaagcggcaa gttcgggcaa aaggtcatcc caatcatgta
     7141 aggatgcagc aagcccatgt gtgagaatga caggtgcccc cctgccctgt tgcacataat
     7201 ttacaaactg cccgctttgc agcaccccac tgtgtgttgt tatctgatcg atcatttcca
     7261 ttttctcaaa aatccttttt acaagacaag ggcgaagttt ttcatccttc atcctttatc
     7321 tttcatcctt gttgttttac cctattctcc tgcaatttgt tttccatagc gtttggccag
     7381 ctcacgccgt aataccttgc caatgaaggt gcgtggaaaa tcatccatga agatcacgaa
     7441 gcgtggaaca aggtgcgggg gcaggcggcg tttgcaatat gcaatgaccg attctgcact
     7501 tggtttctct ttgccagcaa tgacaaaggc aaaaggatgc cccgccaccg caacgaccac
     7561 ggtttccttg acctgcggaa tttcgtagat cacttcctcc acatcgcgcg ggaaggcagg
     7621 tgcgccaggt ttttcgggat accacatgtc agccttgcgc gcgacgatgc ggaaatatcc
     7681 ttcttcatcc atttgagcca catcgccggt aagcagccag ccatcgtcgg taaggacttc
     7741 ccgtgtggcg gcttcgttct tccagtaccc catcatcacc tgcggaccac gcacagccag
     7801 ttcaccgatc tgcccaggct ccacttcttt gcggcccgat ttcagatcca caactgcggc
     7861 gtgtgtggat ggcagcggaa cgccaatgct gcccactttg cggttctttc caagcgggtt
     7921 ggcgtgggtg atcggcgaag cctcggtcag gccaaaacct tccacaagtt ttcctttggt
     7981 gagcttttca aaagattctt gcacttccac atgcaaaggc gcagaaccgc tgatgcaggc
     8041 tttgatggaa ccaatgccat atttcctcac accgcggaaa ttggtgatcg ccacatacat
     8101 atttggcgta ccggggaaga tggtcggctt gtattttttg atggtcttca aaacttccag
     8161 agcctgaaat tgtgccttca agatcatggc cgcaccaagt gaaacaggga cattcaatgc
     8221 agcggtcatg ccataactat ggaagaaagg cacaacacat aaaaaccgct cacggccttc
     8281 catggcttcg ggcagccagt gacgggtttg gagtgcattc gccacaaggt tacgatgaga
     8341 aagcatgaca cccttggatt gtgccgtggt gccgccagtg aaaatgatgg ctgcaaggtc
     8401 ttcaggctgc acttccacag cgggagattt atcgctttgt gcggagagcc agcgattcca
     8461 atgcagggcg ttttgcacat ccaacccgcg gttgcgccag cgtgagatga gatattttgg
     8521 aagcgggaga tattctgcgg ggtcggtcag caccacatgc ggcgtccctg catcccgttg
     8581 aatttgtttc gccagacccg accacattga aagagtaacc agcacactgg catcggcttc
     8641 tttgatctgt cgtgtgagtt cctcgggttc gatcatcggc ggaatcaaca caacagccgc
     8701 acccgccttt aacgcgccgt aaaatccgat caccatttgc ggcacattcg gcaaaagcaa
     8761 aaccacgcgc gcgcctttac cgataccctg tgcaagcagg gcatttgcaa agcgattcgc
     8821 ttcatggttc aattttcgat aggtcagttt tgccccttca aaaaagatgg cggcatggtt
     8881 cgggaagcgc cgcaccgaag aacgcagcaa atgatgcgcg gggatgcgcg gtacatccac
     8941 cgtgtagggc acgccatttt cataatggtt cagccaaaca cgctggctct tcaattcgtc
     9001 acgggtggat ttggaaacga ccagggaggc atcgtcacgc caggattttt tcgtttcccc
     9061 tttgagaaaa cgggcgatgg cacgatcgac cgcttcgcgg cgttccaaca tgaccatgtg
     9121 gcctgatacg ccaatatcaa catcttctcc cccgggaatg gatcctgcca ccttctcgaa
     9181 cagtggccgg tcgaagacaa tgtcacgatg tccgcgcacg atcatggtag gcacttgcag
     9241 ttttgcgaag cgttcccatc ccacccattt ggaaaggttc tcaagataga acgggcgcaa
     9301 ggcatgcgga ggggcatgca gccattttcg cgtgaatggt ccgatcaggc gcaaaatgga
     9361 agcaggcagg ttcaacgcaa atttgaacag gggcctgagt ttgaattcgc cagcggtggc
     9421 gatgatgatc aatttttcaa tatggtcggg gtgattgagg gcatattcag tgacaaccgc
     9481 accgccgaag gaatggccga tcaacacaaa aggtgtggac actttcaatt gtgtcagagc
     9541 agtttccagg tccagttgga tgcggggcat gtcgtagcca gaggttggct tgtcggagag
     9601 gccatgtccg cgcaggtcaa gtgcaatcac gcggttatcc aatgcgaact tttgcatttg
     9661 atactgccat tgctctgcct tgccgccaaa accgtgaata aaaacgagag tccgttttgg
     9721 aagttcgggt gaaacgtcga tcgccgagag gcgcaccaga ggatctgatg aaattcgaat
     9781 ttcgtggcgg tacaggtcga agtctatgtc cattggagaa gtttacatga aggggtgacg
     9841 aatgtcaatt tacggagagg ttcgataatg ctatactaaa aaatgcgctt ttttttcagg
     9901 catccacaaa aactccagcc gacttataaa taattgaggc ctgccatggt tgattccact
     9961 gattcttttt ttcgagggcg ctcttacaca ataatatcct taattggatg gactgcagtt
    10021 actctgggac tcatctggtt ccatcttgga tggttgaatg acctgcacct gtcaccagat
    10081 gaatcaaaca agccctgggg ggattcgttt taccatacct ggggccagga gtatctgtcc
    10141 atattattaa gtggatatgg cacgctttgg ctggtgggct ccatggggat ctatcttgta
    10201 tcgcgtgatc tcaaaaagca atttcgagaa agggaagata tccttctttc ggttcgaaaa
    10261 agccacgaat tgcttttgag cagtgaagaa aaattttcga aggtattcca actttccccc
    10321 gccattatcg cattgaccat gctggaagac ggcacatttt tggatatcaa tcagaaattc
    10381 cttgatatct caggcttcac ccgcgctgaa gttatcggaa agacttcaat ggaaattggc
    10441 tgggtctccc ctgaagttcg tgatgccctt ataaaagaaa tccaaatcgc gggacgagtg
    10501 accaacagag aggtcattct acatgcaaaa aatggggagg aaattctttg catttacagt
    10561 ggcgaattgc tcgaactggg agataaaacc tatgtgcttt ctctggcaca ggatataacc
    10621 gaaaggcgaa aagcggagga ggcactgcaa agcagcgaag cccagtaccg cctgatcacc
    10681 gaaaacatga aggacatggt ctggttgatg gatatggagt tcaagccaat atacatcagc
    10741 ccgtcggtca caaaactgcg tggtttcacc ttgaacgaat tacaggtgct tccattggaa
    10801 aaaaacctgg cccctgattc catgcggcgg gcacttgagg tcatgaatgt gtccctcgcc
    10861 cctgagatga tcgccagcac ccattcctat aagatcgaac tcgaatatta taaaaaagat
    10921 ggaagcactt attggagcga agatacattt gttttgatac gaagcgaaga gggacacccg
    10981 gtcggcattt tgggcggcgg gcatgagatc actgaaaaga aaaaagccga ggatgcctta
    11041 cgcgaaagcg aagaaatcct gcgcacggtg ttcaacacca tggaagaagg cctcgctcta
    11101 aacgagttgg tctacgatga aaacggagaa atcgtggact atcgtattct ggaggtcaac
    11161 ccggcttacg agcgcatcac caatgtgccg cgtgatcagg caatcggcaa actggcaacc
    11221 gagttttatg aaatgcctac ggaatatatc aatgccttct ggaaagagaa catcgataac
    11281 gaccatattg ttacagagct gtacgaagac cgtttgaagg catggaagca catttccact
    11341 tcgcggccca gagacggtaa attcaccact attttcttcg atatcaccga gcagaaaaaa
    11401 tcggaggaag ctttgcgcac cagtgagcag caatatcgcc tgctcttcga agaaatgatg
    11461 gatggttttg ccctgcacga gatcatttgc gatgaaaata acataccgat tgattatcgt
    11521 ttccttgcgg tcaacccggt attcgaaaaa ttgaccaaat taaaagcaga agacgttata
    11581 ggcaaaaccg ttctggaagt catgccagat attgaggatg tctggataaa aagatacggc
    11641 caggtggctc tgacaggtga acctgcccat tttgaaaact tctctacaac cctgcaaact
    11701 cattttgaag tgagggcctt ttcgcctgca cgcggaaaat ttgcagtgat cttccatgat
    11761 gtgacccagc aaaggaatgc ccagatgatc atgctggctc gcatgaaatt cattgagagc
    11821 tccaccgtac aaccgcttga ccgtttactg cagaccattc tcgatgaggc cgaagatatg
    11881 acgggcagca aggtcgggtt ctatcatttt gtgggtgcag atgagcaaac cctgttgtta
    11941 caaagttggt ccacacgtac agcatctgag ttttgcaagg cagatggcaa aggcctgcat
    12001 tatccagtgg aaagagcggg agtgtgggct gattgtgtcc gtgaaaaaag gcctgtcatt
    12061 cacaatgatt acaagtctct gcctcaccgc aaaggactgc ctgaaggtca cgctgagctc
    12121 atacgcgaat tggttgtccc tgtattgcgg aggggaaagg ttgtagcact tcttggggtc
    12181 ggaaacaagg aattcaacta taccgaacag gatgttgcca tcatcggtca acttgcagat
    12241 ctggtgtggg acattgtgga aagcaagcgg gttgaagaga tccaaaaaag ggtcgagcag
    12301 gattatgagg ctctcatcca aagcattgac gggatcgtct gggaagccga tgttcatacg
    12361 ctcgaattct cctttgtcag ccagcaggca gagcgtttgc tgggctatcc catcgaacgc
    12421 tggaccaccg aaccaggatt ctggatcgac cacattcacc ccgatgaccg tgccgcctcg
    12481 atcagcttct gtgtggcaca gacccgcctg aaaaaatcgc atgaattcga ataccgcatg
    12541 ttcactgcag atgacaggat catttggctg cgcgatgtgg tttctgtggt gcttgaaaac
    12601 gaccaccccg taagattacg cggcatcatg atcgatatca ccgagaaaaa aatggcagat
    12661 gatagactgc gtcagctttc attggttgta gaacaaagcc ccgcttcagt ggtcatcacc
    12721 gacctcaatg gcaatattga gtatgtcaac caaaaattta tgcaggtcag cggctacaca
    12781 cttgacgaag tcctctacaa aaaccccaat atcgttcaat caggaaagac ccccaaggaa
    12841 acctatgccc aactgtggaa aaacatcacc tcaggcaagg tttggcatgg cgaatttcag
    12901 aacaaacgca aaaacggcga atactattgg gaagatgtgc tcatttcacc gctgctcgac
    12961 accaacggaa agatcaccca ctttgtcagc gtgaaggaag atatcaccga acgcaaacag
    13021 gcggagaatg agatcatcga aaaatcgcag gaactggaag ccctgttcaa gatttcatct
    13081 catttgcgca cggcccaaac cgtacccgaa atgatccccg tagtcctcaa acaaatgcgg
    13141 gaaacattta actcagatgc gaacgctgtc attcttatca acagggacca cactcaattc
    13201 aaatatgttc tcggtgatgg cctgttggct gaaaacacag ggctgcaatt tggcattgag
    13261 cacagcatca gccgccgtgt aatggataca ctcgaaccct acatcaccct tgattttaca
    13321 agcgacctga tcggcgcatc cgcggtgaag gtcattgaga atattggagc aacggtcgtc
    13381 gtacccttgc aatcagaaac ggaattcctc ggcactttat tatgtgcgcg ggcaaaagac
    13441 tctcagcttg cgccattcac ctctgctgaa atcaaactac tgcgggccat cggcgaaatg
    13501 gttggaaatg cgctaaggcg ggcaaaactc tatgacgatg ccctctcccg cctgcaacgc
    13561 gtggaagcct tgcgctccat agacatggcg atcaatgcaa atatggatgc attgcttacc
    13621 ctgaacatgc tcctcagcca gaccatcgcc ctcttaaaag tggatgccgc cgccgccatg
    13681 ctttacaacc cagcctccca tatgctcgaa tacggtgcag gacagggctt tcatcacaaa
    13741 gaaatcgaaa aagcgaatca aaaattaaat cgcggctttg caaatactgc cattattgag
    13801 cggcggcttg taacagcccg caacctcaaa ctgaccgaag accaaagtta ccgtgcgatt
    13861 gcgatcaagg aaggttttga aacctgtcac atcgtcccga tgatcgccaa cgggcaggtg
    13921 tgcggcatcc ttgaggttta caacaaaacg gaaatctccc cagaccagga atggctcgat
    13981 tttctcgaag ccctcggcgg acaggcagcc attgccattc acagcgcaca gcttttcgag
    14041 aaccttgaaa aatcaaatct cgaactctcc ctcgcctacg acgccaccat cgagggttgg
    14101 tcgaaagcgc tggaactgag agacaaggaa accgaaggac acacactgcg cgtcacagaa
    14161 tggacggtta aacttgccat gttggcaggt atgggcgagg ccgatatcgt tcaagtgcgc
    14221 cgcggcgccc tgttgcacga tatcggtaag atgggcatcc ctgaccatat cctaaacaag
    14281 aacggcgaac taacctctca ggaatgggag atcatgcgtc agcacccgca atatgcatac
    14341 aacatgatcc accccatttc attcctgcgc cccgccattg aaatcccgta ttatcaccat
    14401 gaaaaatggg acggcggggg atatccattt ggattgaaag gggagcaaat tccctactcg
    14461 gcccgtctat tcgccatcat tgatgtctgg gatgccatta cctcgaaccg cccctatcgc
    14521 aaggcgtggt ctttcgaaaa agcattggaa tatataaaat cggaatcggg caggcacttc
    14581 gacccaaata tcgtcgaact ctttttgagg atggtcaacg gatttaggaa tgaatgaccc
    14641 aaagcccgcg cttcgccgct gacctcgcaa ctggtcgggc gaaattgaca ttcaaggagt
    14701 ctggagatga gtctcgaaaa agcagtggaa gccatcatca aagaagccca ggaacgcggc
    14761 gattttgaca acctgaaagg caagggaaaa cccattgacc tttcagctta ttttgaaacg
    14821 ccagaggaac tccgcaacgc gcatgccatg ttgaaaagtg cgggcatggt ttctgccgaa
    14881 gtggacctct tgcaagagat cgccgccctg aaagaaagac tcgcagccac ctacgaggaa
    14941 aaagaccgca gccgcatcaa gaaaataatc cgcgacaagg aattgcagtt caatgtaatg
    15001 ctcgaacggc aaaaacgtca gcgcaagaga aaataacagg aaacaggagc aggcgtcggc
    15061 ggggagggat gcggcccccc gttcagcaga aaaaagccgc tttgacaaat catccccgcc
    15121 ctgctatact cttaacagtt aagttgactt tctctgccat tcaggcagtt tgtttgagaa
    15181 caatcgtgtt ttgaaatacc gatccttcgc gagcaagtca gcggaggtct tttttttgtg
    15241 ggaaaagact ccgaggaacg gaccagaaat atatcaaccc tgacttttac cgcataagcg
    15301 gtttaacata tcaactcttt gtgaggttta taaaaaatat atgagtaaaa agaacaaatt
    15361 aagaattatc ccgctcgggg ggctgggcga agtgggaaag aacatgatgg cctacgaatt
    15421 cggcggcgac atcatggtgg tggatgcagg aatcatgttc ccgcataacg acatgctcgg
    15481 ggtggattac atcatccccg actttgagta tttaatgaaa cgcgccgacc gtgtgcgcgg
    15541 gctggtcatc acccatggtc acgaagatca tatcggcgcg atccagcatg tgatcgacca
    15601 gatcaatgtg cccatctacg ccaccccgct gacccgcggg ctgatcgagg ggaaattatc
    15661 gcgcaacaac gcccagcaca aggcccagtt taaaaccatt caggctggcg aatcttccaa
    15721 tattggcccc ttcaaggtgg aatattttca tgtcagccat tccattccag atgcggtggg
    15781 attgggcatc accacacccg ctggtttggt cgttcatatg agcgatttca aatttgacca
    15841 tacccccgtg gatggctggc cgaccgattt tgcaaaactc tccgagtttt ccacccgcgg
    15901 tgtggacttg ctgctttctg attcaaccaa tgccgaacgc cccggttgga ctccatcaga
    15961 gtctgtgatc ggccccgcat tcgataaggt ctttgcagaa gcaaaaggcc gtgtgatcgt
    16021 ggcgaccttt gcttcgttga tttcacgcgt tcagcaggtg gctgatgccg ctgcgaaaca
    16081 tgggcgcaaa atggcccttg ctggccccag catggtggat aatgtaaaga tcgcccgcaa
    16141 gatgaagtat ttgaacatcc ccgatgagat gatcgtaccc attgaccagg ctttgaacat
    16201 gcaggaccac aaggttgcga tcatgtgtac tggctcacag ggcgaaccct cttccatcgt
    16261 cgggcgttta tcggctggga caaatcgtca atttgacctg aaacccggtg acaccattgt
    16321 gctttcgtcg cacccgattc cgggcaacga agaaaccatc agcaaaacga tcaaccgcct
    16381 gttgcgccga ggcgcacggg tgatccatga tgggattgcc cccatccatg tttcgggtca
    16441 tgcggcgcag gaagagcaga aactgctcat caaccttgtg aggccgaagc atttcatgcc
    16501 gatccacggc gagttgcgtc agctcatgcg ccacgccgaa ttggcgcaac aacttggaat
    16561 tgaacctgaa aacaccatcg ttgtagaaaa tggacaggtg gtggaactgg tcggtaacaa
    16621 gatattgctt ggcgaacgca tcccaggtga ttatgtattt gtcaccggtg aatccattgg
    16681 cgatattgac cacgacgtga tgcgcgaacg cagccaactg gcccgcaacg gcatcatgct
    16741 gatcgacatc agtctcgata aactcacggg caaattgctt catgaccccg aaatcatcac
    16801 ccgcggattt gtctctccag aagatgctga aatcctgatt cctgaagtac aaaaacgggt
    16861 gatggatgtc gtccacagcg gcgggttgga cgatgaaaaa gacattgtca atgctgtgaa
    16921 gagctacctg catcaggaaa ccaaacgcag gcccatggtg tttgtgacct tgagcaaggc
    16981 gtaaaatttg aaaagtagaa agatgacacc ccgtcgattg gcggggtgtt tttttgaagg
    17041 ctattttata aatttcaggt ctgaaacgct ggcatccaaa tttgtacttg gcagggaacg
    17101 atatccgaca aacatgtatt taatcgcaaa actcaacggc cactcagaga taataccctg
    17161 accatccaca gacgccttga attttccttc cctcaacaca atatccacat tgaacacacc
    17221 gtcggcaaat ggcaaaccga cattattcac aggcaggtct tccacaggca attgccgaat
    17281 atcgatcaca tcatcaggtt gaatcacaaa tacgatcccg ttcgagtaca cggtctcatc
    17341 ttcaaaaaag ccgacccaca cctcgctgtt ttcaaggtgg cttttatgca ctgaaaactc
    17401 cacccctttc cagtcctgcc acggggtgaa tattccatac tccaccgcac gcgattgaga
    17461 tttatgggta acaaagatca ccttgccgct ttcacctgta attccccatt gtgtcagttc
    17521 gtagcagggt gtcgatcccc tcgtgatctt atctgctaaa aacgtcgacc aaattgtggt
    17581 gtcaatacaa ttccgcgccc aatcttcacc aggcacggga ggcggcagca caggagccac
    17641 caactctggg gttgcaggag caggaatcgg cgaaggcgtt tccgttggtt cttccgcctg
    17701 acttacggca gggggtaaaa catccgtcgg tagcgcggaa gttggggtgg gggtttcttc
    17761 ggaaagtatg cgggggaaaa caaaaggaac agcggctacc gcaattgttc caatacaaat
    17821 aacgatctgc acccaaacag ggataaattg aacaaggcga tcccaaaagc tttttttagt
    17881 ttgactcttt ttacttgacg gactcatcca tgcctcccaa acaccgcagt attgttttct
    17941 tcgtagtttc agtataacga aaattttctc aagtgcaata tcacgccagc attttcgaaa
    18001 tttgcctatc caccttgccc atggggaaat cacccaacgc a
//
LOCUS       DAOQJC010000084        17914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1480_length_17914_cov_9.193180, whole genome shotgun sequence.
ACCESSION   DAOQJC010000084 DAOQJC010000000
VERSION     DAOQJC010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 17914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17914
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1480_length_17914_cov_9.193180"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PLE39_12850"
     CDS             <1..464
                     /locus_tag="PLE39_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69264.1"
                     /translation="LMGGDVPAKLDMDNLQSEKSFDGLNTYSQTKLAMMVLMYEFAQH
                     EKGMTINVCYPGQASTNMTRSVTPEMLPRAMRWMFPIFKLLVREDGGKSAAKAARSSV
                     YLASSPEVEGVTGKYFDTNCKEAAWSPAVMDDDMRKTLWEQVEQITKRQSG"
     gene            complement(492..1982)
                     /locus_tag="PLE39_12855"
     CDS             complement(492..1982)
                     /locus_tag="PLE39_12855"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNE69265.1"
                     /translation="MADQKHREIFSQGRKVWNAWRKKNPKIKPDLSTLSFRNADLGGF
                     DLRAVFLENSDLRNAQCVGTDLRGAYLYNVDARKANFDKANLSGATLQRALFQGASLR
                     NAKIEFTLSQEANFEKADLTNASLFLSDLSDAKFDQAILKNANFNETNLNGTYLTQCD
                     LSSCTFKKASLVNVKMVNSNLTGVDFSGRTLAGASFWRANLTRANIKETDCQMVEFAE
                     AKLVGANLSFSNLDTAEFRDADLTSADLHGSYLANTSLLRAVVEKANFKNCYVYGTSA
                     WDLIGTPKSQQDLIITPPSEPAITVDDLEVAQFVYLVYNNKKIRNFMNAFTDKNVLIL
                     GRFSLPERKAVLDGLREKLRTFDFVPIVFDFDSPQDKDYTETVQTLAGMSMFVIVDVT
                     NPKSTPLEMEATVKQFKIPYLPIIDTSADDRPFAMMIDLQKSFHWVLPTFGYKTRKEL
                     LSNLKAAIIDRAIEKHDQLRDQKAKESISMLTVDDLKKRSGAKRKK"
     gene            complement(2065..2697)
                     /locus_tag="PLE39_12860"
     CDS             complement(2065..2697)
                     /locus_tag="PLE39_12860"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNE69266.1"
                     /translation="MPLLLLIRHGENEYVKTGKMAGRLPGVHLNERGQKQAQALGDAL
                     KDVPIKAIYSSPLERAMETAAPIASARNLKIIQEPDLMDTDIGKWQGKSWRVLGLTKA
                     WKIVQNAPSRFRFPEGESFPEAQLRIANVLERVVQTHKKPQDIVAVVFHADPIKLAVS
                     HFIGMPLDHFQRLACDTGSMTVLHVGEAGAHLVKLNQRPPFDFLPKENKK"
     gene            complement(2712..3161)
                     /locus_tag="PLE39_12865"
     CDS             complement(2712..3161)
                     /locus_tag="PLE39_12865"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HNE69267.1"
                     /translation="MDEIRPWLFIGGFRDTLDKGYLDRKLIRSMLQIAEPIQHEGINS
                     LFLQVDDLARTPHHLIKQGVDFVLAEKEKGHNILVSCGAGVNRSTMFCLAALKEAEGL
                     SLFDAFKEVSQRHPGALPNEQVWEAFCDYYHESVPYLDVMRLAAKNF"
     gene            complement(3163..4569)
                     /locus_tag="PLE39_12870"
     CDS             complement(3163..4569)
                     /locus_tag="PLE39_12870"
                     /inference="COORDINATES: protein motif:HMM:NF009883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE69268.1"
                     /translation="MSDLFDHAMQERMKTEAPLAARMRPRTLDEYIGQEHIVGEGKLL
                     QRAIKADKLFSSIILWGPPGTGKTTLAQVIANSTKSHFVTISAILAGKADLRVVIDEA
                     LERRRLHNERTILFVDEVHRWNKAQQDALLPHVENGTFTLIGATTENPYFEVIKALIS
                     RSRVFQLRNLNQTETGVLLDRALSDKERGYGNKRITLDLDARSHLIEVASGDARNALN
                     ALELAVESTTPDRDGIIHITLDVAQESIQRRAVLYDKDGDAHYDTISAFIKSVRGSDP
                     DAALYWLAKMIYAGEDPKFIIRRLIILAGEDIGLADPMGLVVASSAAQAFDYIGLPEG
                     IYPIVEATLYLATAPKSNSAGAYFSAMKKIEEEGQVSVPRHLMDGNRDAAAMGHGKDY
                     VYPHDQEGHFTPQQYLPRRLLGTYFYSPSQEGYESQVTARLEMWREAQRKALNIERTE
                     HVPSLSEEQIVEMKRKIK"
     gene            complement(4573..4986)
                     /locus_tag="PLE39_12875"
     CDS             complement(4573..4986)
                     /locus_tag="PLE39_12875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNE69269.1"
                     /translation="MKRAVLDTNILISSALGGALVLVLEKWDAGVFSVVVTTDILSEY
                     FEVLNRPKFGLKQETIDKITTYIYQFSEFVIPEEKIRFIEDDPKDDKFLEAAIAGNVD
                     FIVSGDKHLLDLKEFRSVPILSGREFLDWLQRNGN"
     gene            complement(4983..5216)
                     /locus_tag="PLE39_12880"
     CDS             complement(4983..5216)
                     /locus_tag="PLE39_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69270.1"
                     /translation="MTTFEQTLLREVATLPQSRQADVLAFIRFLKISLPESEKARSDF
                     KEALEDARATAKKLNITQEDIEAEIRAVRDGKQ"
     assembly_gap    5412..5511
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5512..6748
                     /locus_tag="PLE39_12885"
     CDS             <5512..6748
                     /locus_tag="PLE39_12885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HNE69271.1"
                     /translation="AREELIAFLQKLVQTASLPNHEHDVQNLVAQKLRSMGMEVEIIP
                     SNFDNLKGHPAFGDDGFSPTDRVNVIGRWPGTRTGEGKSIILNGHVDVVPVGDLSLWV
                     DSPWSGVIRDGKMYGRGSCDMKSGLCAGIFAVDVLQKLGYQPANDILIESVIGEESGG
                     IGTLTTIVKGYTADAVILLEPTRLELSPVQSGALTFRLTVPGRSIHAAMKAYGVSAIE
                     KFALLLNVINKLDAERHASFKHPLFEDPHNIAPINIGTVRGGEWHSTVPEEVVAEGRM
                     GVFPGESNEAARKMFEAAIQTAAESDPWLSQNPPRVEWFEGQFESGETPLEHPLIQNL
                     AAMHEQVTGSAATLRGVTYGSDMRLFTNHAKIAATHYGPGDVGMAHAVNEFVPLDEVV
                     TAAKVVANTVAQWCGGEFK"
     gene            6745..7623
                     /locus_tag="PLE39_12890"
     CDS             6745..7623
                     /locus_tag="PLE39_12890"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HNE69272.1"
                     /translation="MKTFGCQSMYAPIRRMVLKHPREAFISQANLEAQWEKLVYFGCP
                     DFENSLAEYERFTGILEHFDFEIHYAPMDASTSLDSIYTHDPLVISNRGAILCSMGKE
                     ARKPEADAAEKYLHELGVPILGRIHGSGKLEGGDVLWVDERTLAVGQGFRTNAEGLRQ
                     LRELLSDAVDEIYPVQLPYWTGPQDCLHLLSFISIVDKDLAVVHSRLMPVPFREWLLE
                     RGFRFVEVPNEEYDNLGCNVLAVGPRQCVMLSGNPITQKRLEEAGAQVWTFDGKDISF
                     KGTGGPTCLTRPILRS"
     gene            complement(7677..8075)
                     /locus_tag="PLE39_12895"
     CDS             complement(7677..8075)
                     /locus_tag="PLE39_12895"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE69273.1"
                     /translation="MEIKITTMKGRVPVQVMHVHGELNSATYEAFQSKANELIKSGAQ
                     YILLDLTNSPFISSAGLRTIHSIYNQLRAAKPDKDDEEIRKGISAGTYKSPYIKVLNL
                     SSDAAKAFNLGGFDMYIESHTDLLKAVASF"
     gene            complement(8180..9202)
                     /locus_tag="PLE39_12900"
     CDS             complement(8180..9202)
                     /locus_tag="PLE39_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69274.1"
                     /translation="MQLDAIIEVAIGLVFVWLVISITTMEAQNRLGVWMNGRAKHLEK
                     SIHSMLKDKTLVDRFYDHPLIVELMLKDNDGNLKLDKKGKPRRPDYIPTQVFATAVFE
                     VLMNAGKGEKDIAAETLSIPDMTANFKTLMAKNEVLPYVFPNMEKSMETLQRNTKEFD
                     DKLAEYRRNIEGWFDNVMVQASGWYKIDSQWKAFWIGLALAVFFNVDTIFIAQKLWQE
                     PTARAVIVAQAQTEAQGDSSADSISLDNVKGLVFPVGWTTTPLAEGTSCGLVSFMDNR
                     VVISSEGNCLAVTSLPAFNNGWGLMVKIFGYLLTAFAAAQGAPFWFDILRKLVGFKQQ
                     TSSTSK"
     gene            complement(9281..10171)
                     /locus_tag="PLE39_12905"
     CDS             complement(9281..10171)
                     /locus_tag="PLE39_12905"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE69275.1"
                     /translation="MQNKILPYLALAVGISALSLSAMFVRWAEAPGAVTGFYRLLFST
                     IFLSPLFIRQQKNLEPIEKKYLLFPLLGGLFTAFDFGFWNSSLKFTTAANATLLGNTS
                     PLWVALFAFFVLHEKLRRSFWVGLVIALTGAVLVMGSDYLKHPTLSLGDLMASTAAVF
                     YASYQLVTQRGRKHIDPLRYTWLVGVSAVIGMFIMNLVLGNQLTDYPLKSWLIFLATA
                     VVSQMIGYLAITYALGHLPAYVVSPTMVGQPVLTAVLAIPLLGEVPSTIQWVGGAIAL
                     VGIYLVNQSHLQAQEELPNA"
     gene            complement(10189..10932)
                     /locus_tag="PLE39_12910"
     CDS             complement(10189..10932)
                     /locus_tag="PLE39_12910"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNE69276.1"
                     /translation="MKASSVHHILFDLGGTLMHSRGDWTPILERADQALADSLRQHEI
                     ELDTRVFRARLHEYYDQRDQDFQETTYHFVLRELLKDLGYVDVAEPVLRSALDALYSV
                     TQSNWMLEDDSLDTIQALRSNQYQLGIFSNAGDDQDVQELIEDFGIRSYFDFVLTSAA
                     CFYRKPHPRAFEIALAQWNISPEEAVMVGDSLEADIQGAKNLNMKSIWITRRAQFKAD
                     EMQRFKPDFSVRSLKELLPTLERISITRA"
     gene            complement(10971..13022)
                     /locus_tag="PLE39_12915"
     CDS             complement(10971..13022)
                     /locus_tag="PLE39_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69277.1"
                     /translation="MKVFQFLFRVGFSLSIAFALTFSSAIIFFDYSGYETALDRAALV
                     SFPTLAIGFLLFQAFPTLWQWIRQRRPAVLFLLGGLAGIGAAAVVLPFAVSSVYYLGT
                     LAFALALFALMLPAAPMVERMRNRLWGYSFGFLLSLLFAYGAVGFLAGALHSIFNVLV
                     FTVILVGAGSVAGNYLFIRAADSFRGGFLRQPLNLLLALALPAFLAAVIAVGMQFPPM
                     LLRQHLMVPREWFGLFLSAALMTGVWGLGLLEQFETRGFHNRLEKTRLFEFIKPNLPG
                     VYAGGMFFLINLVIARILNHPALSVNTVLFESDAGPWLFILGSPTGDALNRSVHPLAL
                     TTIRYLVRVFAIFMGDAWQLAPILVAAMLSGLCVFMAWLFVKRAVGKSSYAFIFAVML
                     GSTAAHLFFGSLTDTYIFGVTSLIFFWLLIQAQENRFAVLVPAGVLVFGVTVTNIAQA
                     MIGLFCSKFGFRRLIRYGIMVLAVSIVLTAFTSMVYPRWQTFFFVPADLAFEGNFVKP
                     VYESPTERLLERFKLVGRSMVLYGVTAPSPIEVIAEKDPRPTIDLKTFDAPNNAYASY
                     KGFANIPLALWLALLAGAFIFFMKGLRSSAHLSLMLGLLGSLAFNFILHMNYGTELFL
                     YALYWTYLLVFFLALAYAGLAGRPWFEAILTLFILALMANNLGFIFAVLRGLAPFFAS
                     V"
     gene            13208..15181
                     /locus_tag="PLE39_12920"
     CDS             13208..15181
                     /locus_tag="PLE39_12920"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE69278.1"
                     /translation="MSGLIGQSLGRYQIVELIGEGGMATVYKARDARLDRDVAIKFIR
                     PEAVPPDQFEIILKRFEREAKSLAGLSHPNIVGVIDYGEHDGLPYLVMVYLPGGTLKE
                     RLGAPMYWRDAVRFIIPVARALEYTHEHSIINRDVKPSNILLTEKGQPMLTDFGLVKI
                     FEGKNATHLTASGAGLGTPDYMAPEQWIGEATAASDQYSLGVVLYEMITGHRPYISDT
                     PAGILLKQINDPLPFPKTYIPDLPQSVESVLMKVLARKPTDRYADMHAFANELQNLLD
                     GREVTATGIKTKQLREQMTGKIEKPKIEQKPAEEEKAPKKTHLQADQKPASPADPQPA
                     SPKKKISSPWLAVLGGFVLLGLCGACWLLNARFNFFGGTTNPTATQSVTLATDVPTEE
                     VVEEILPTETSQPASEPTEAPATEISLPAEITDGKNISMRLVPAGEFIMGNDDGNTEE
                     SPASKVQVAAFYMDKYEVTNDMYAECVAAGVCKLPEKPGSATRSSYYNNPSFANYPVL
                     YVNWTMAKTYCEWRDARLPTEAEWEKAARGMDDQRAYPWGNDFNCNFANHSGCVGDTA
                     PVNQYNSGQSPYGIYGLSGNVWEWTSSLFAFYPYSATDGREDPTATGFRSVRGGSWNI
                     FGGSGGNIRIDTRYKLDPVYFGAYVGFRCALTK"
     gene            15533..>16628
                     /locus_tag="PLE39_12925"
     CDS             15533..>16628
                     /locus_tag="PLE39_12925"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNE69279.1"
                     /translation="MATVYKAYDTHLEREVAIKVIRRDAFPPDDMNMLLKRFEREAKS
                     LGRLSHPNIVGVIDYGEHDGSPYLVMVYLPGGTLRERLGKPIAWRSAVELILPIAHAL
                     EYVHDRNIINRDVKPSNILMTEKGQPMLTDFGLVKLFGEQEKESTHLTSSGTGLGTPD
                     YMAPEQWTGEAIPQSDLYSLGVVLYEMITGRKPFTADTPAGVLLKQATEALPLPRAYI
                     PDLPQEVESVLLKVLAKDPEDRYPNMHTFIGELQNLLAGREVSATSIKADQLRKKMTG
                     RTEATTFDPATGRTQKAFPTLFAVLGGLGALMVIAVIGVLIYAGNGGTALPFLAAPTQ
                     TSQPTAIPSATNTAIPPTETVEVTAAPTETL"
     assembly_gap    16629..16728
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16729..17205
                     /locus_tag="PLE39_12930"
     CDS             <16729..17205
                     /locus_tag="PLE39_12930"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE69280.1"
                     /translation="VFKDYPVIYVDWKWAKAYCEWRGARLPTEAEWEKAARGTDGRLY
                     PWGNEKPDCNLANIAGCVGDTTPVDQHELGKSVYGVYGMAGNVMEWTSSLFDYYPYDA
                     ADGRENPDSTKERIVRGGAWNLFGGNSGNVRVDTRVKLFPESAVAYVGIRCAKSAQ"
     gene            complement(17276..>17914)
                     /locus_tag="PLE39_12935"
     CDS             complement(17276..>17914)
                     /locus_tag="PLE39_12935"
                     /inference="COORDINATES: protein motif:HMM:NF025888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE69281.1"
                     /translation="SVGCGWSSINADIRIVNAQTKELCAKDEVGEIWVSSTSVAQGYW
                     KRPEETEKTFQAYIASSNEGPFMRTGDLGFIYNDQIHITGRIKDMVIIQGRNFYPQDI
                     ELTMEKCHDALRPSFGAAFAVEANQVDHLVVVQEVKREYRKSNDFEGIANAIRMAVAK
                     NHGLRVQAVVLIMPSTIQKTTSGKIQRNATRESFLKGNLEVLYEWRAPGIFS"
BASE COUNT         4609 a         4762 c         4385 g         3958 t
ORIGIN      
        1 cgctcatggg cggcgacgta cccgcgaagt tggacatgga caacctgcaa tcggaaaaat
       61 catttgatgg attgaacacc tactcacaaa cgaaactggc gatgatggtc ttgatgtatg
      121 agtttgccca acacgagaag ggcatgacga tcaatgtctg ctacccgggg caagccagca
      181 ccaacatgac ccgcagtgtg acacccgaaa tgcttccgcg cgccatgcgc tggatgttcc
      241 ccattttcaa gttgctggta cgcgaggatg gcggcaagtc cgcggcaaag gcggcgcgtt
      301 cgtctgtgta ccttgcgtct tcgccagagg ttgaaggcgt tacgggaaaa tacttcgata
      361 cgaattgtaa agaggcagca tggtcgccag cggtgatgga tgatgatatg cgaaaaacgc
      421 tttgggagca ggtggaacaa ataacaaaac ggcagtcggg atgactgccg ttttgatttg
      481 gggttgaagt tttatttttt tctctttgcg ccagagcgtt tcttcagatc gtcaacggtt
      541 aacatggaga tgctttcctt tgccttttga tcgcgcaact gatcatgttt ctcgatggcg
      601 cggtcgataa tggcggcctt caagttactc aaaagttcct tcctggtttt ataaccaaag
      661 gtcggcaaaa cccaatggaa gctcttttgc agatcgatca tcatggcaaa gggacgatca
      721 tcagcgctgg tatcaataat gggcaaatac ggaatcttga attgcttgac cgtagcttcc
      781 atttccaaag gcgtggattt ggggttggtc acatctacaa tgacaaacat gctcatgccc
      841 gccaaagtct gaacggtttc ggtgtaatct ttatcctgag gcgaatcgaa atcgaacaca
      901 atggggacga aatcgaaagt gcgcagtttt tcgcgcaatc catccagcac ggctttgcgc
      961 tcggggagtg agaacctgcc caaaatcagc acatttttat ctgtgaaggc attcatgaag
     1021 ttgcgaattt tcttattgtt gtaaacaagg tacacaaact gggcaacttc cagatcatcc
     1081 acggtaatgg caggttcgga tggcggtgtg atgattaaat cctgctgcga ttttggcgtt
     1141 ccgatcaaat cccaggcaga ggttccatac acgtaacagt tcttgaaatt ggctttctcc
     1201 accacagcgc gaagcaggga tgtattggca aggtacgagc catgcagatc ggcacttgta
     1261 agatcagcat cgcgaaactc agcagtatcc agattggaaa aactcaagtt tgcgccaacc
     1321 aattttgcct cggcgaactc aaccatctgg caatcggttt ctttgatatt tgcccttgta
     1381 aggtttgcac gccagaaaga tgcgcccgcc aacgtccgcc cggaaaaatc cacaccggtt
     1441 aggtttgagt tgaccatctt cacattgacc aatgaagcct tcttaaaagt acaagaagag
     1501 agatcgcact gggtgagata agtcccattt aagtttgttt cgttgaagtt cgcgtttttg
     1561 agaatcgcct gatcaaattt agcgtcagaa agatccgaaa gaaaaagaga ggcgttggtc
     1621 aaatctgctt tttcaaagtt ggcttcctgc gagagggtaa attcaatttt tgcattccgc
     1681 aaacttgccc cttgaaacaa agcccgttgc aaagtggcgc cacttaagtt ggctttgtca
     1741 aaattcgctt tgcgcgcatc gacgttatac aaataagcac cgcggagatc cgttcccacg
     1801 cattgggcat ttctgagatc agagttttca agaaataccg cccgcaggtc aaagccgccc
     1861 agatcagcgt tacgaaatga gagggtgctc aaatctggtt ttatttttgg attctttttc
     1921 cgccaggcat tccaaacttt gcgtccctgc gaaaatattt cacgatgctt ctggtctgcc
     1981 atacaaactc cttttaacaa cgctaaattt caagcaaata aaatgccatg gcacacattt
     2041 ccttcactcg atcaaagctc acagctactt tttattttct ttcggtagaa aatcgaacgg
     2101 tggacgctga ttcaatttta caagatgcgc acccgcttca cccacatgca aaacggtcat
     2161 tgaacctgta tcacaggcca gccgctgaaa atggtcaagc ggcattccga tgaaatgaga
     2221 aacggccagt ttgatcgggt cggcatggaa cacaaccgca acaatatcct gcggcttttt
     2281 atgagtttgc accaccctct ccaacacatt tgcaattcgc agttgtgcct caggaaacga
     2341 ctcgccttcg ggaaaacgga atcgcgacgg cgcattctgc acgattttcc atgcctttgt
     2401 caggccaagc acgcgccatg attttccctg ccattttccg atatcggtat ccatgagatc
     2461 aggttcctga atgatcttca aatttcttgc ggatgcgatt ggcgcagcag tttccatcgc
     2521 cctttccagc ggacttgaat aaatggcctt gatcggcaca tccttcagcg catcacccaa
     2581 cgcctgagcc tgcttctgcc cacgttcatt gagatgtacc cctggcagtc ggcctgccat
     2641 tttccctgtt ttgacatatt cgttttcgcc gtggcggatg agaagtaaaa gaggcattgt
     2701 ttattttcca atcaaaagtt ttttgccgcc agcctcatca catcaagata agggacggat
     2761 tcatgataat aatcgcaaaa cgcctcccac acctgctcat tgggaagcgc gccagggtga
     2821 cgttgagata cttctttgaa cgcatcaaaa aggctgaggc cttctgcctc tttcaacgca
     2881 gcaaggcaaa acattgttga ccgatttact cctgccccgc aagaaaccaa tatgttatgt
     2941 cccttttcct tttcggccag aacgaaatcc acaccttgtt tgatgaggtg atgcggcgta
     3001 cgggcaagat catccacctg tagaaacaga gaattgattc cctcgtgttg aatcggttcg
     3061 gcaatttgca gcatggatcg aatcaacttg cgatcaagat accccttgtc gagggtatct
     3121 ctgaacccgc caataaaaag ccaggggcga atttcatcca tgctatttga tcttccgctt
     3181 catttcaacg atctgttctt cgcttaagct ggggacatgt tcggtgcgtt cgatgttcag
     3241 ggccttgcgc tgggcttcgc gccacatctc caaccgcgca gtgacctgtg actcgtatcc
     3301 ctcctgtgaa ggcgaataga aatacgtgcc gagcaatcgc ctgggtagat attgctgggg
     3361 agtgaaatgc ccctcctgat cgtgcggata cacataatcc ttgccatgac ccattgccgc
     3421 ggcatcgcga tttccgtcca tgaggtggcg cggaacggag acctgcccct cttcttcaat
     3481 ttttttcatc gcgctgaaat aagcgcccgc gctgttggat ttgggcgcgg tggcaagata
     3541 gagggttgcc tccacaatcg ggtaaatccc ctcgggcaga ccgatatagt caaatgcttg
     3601 tgcggcagaa gaagccacca ccaaccccat ggggtcggca agcccaatat cttcaccagc
     3661 aagaataatg agacgacgga tgatgaactt gggatcttca cccgcgtaga tcatctttgc
     3721 cagccagtag agggcggcat cggggtcact tccgcgcacc gatttgataa acgcggagat
     3781 ggtgtcgtag tgcgcgtcac catctttatc gtagaggact gcccgccgtt ggatcgactc
     3841 ttgcgccaca tccaacgtga tgtggatgat gccatccctg tctggcgtgg tggattccac
     3901 tgccaactca agtgcgttga gcgcgttgcg cgcgtcgcct gaggcaactt caattaagtg
     3961 ggaccgtgca tcgaggtcca gcgtgatgcg cttgtttccg tagccgcgtt ccttgtcgga
     4021 gagggctcgg tcaagcagaa cacccgtctc ggtctggttc aaattccgca attgaaagac
     4081 acgcgaacga gagatcaaag ctttgatgac ttcaaagtat ggattctctg ttgtcgcgcc
     4141 gatcaacgtg aaagttccat tttcgacatg cggcaggagt gcatcttgct gcgccttgtt
     4201 ccaacggtgg acttcatcta caaaaagaat ggtacgctcg ttatgcaggc ggcggcgttc
     4261 gagagcctcg tcaatgacaa cgcgaagatc cgccttgcct gcaaggatgg cagagatggt
     4321 aacgaaatga ctcttcgtgc tgttagcaat gacttgtgcg agtgtggttt tgcccgtgcc
     4381 tggcggcccc cataaaatga tggacgaaaa aagtttatcg gctttgatgg cgcgctgtaa
     4441 aagttttccc tcgccgacaa tatgctcctg cccgatgtat tcatccagcg tacgcggacg
     4501 catccgcgcg gcaagcggcg cttcggtttt catgcgttcc tgcatggcgt gatcaaaaag
     4561 gtcgctcata aactagtttc cgttcctttg cagccaatct aaaaattccc ttcctgaaag
     4621 gattggaacg gagcgaaact ccttcaagtc aagcaaatgt ttatctccgc ttacaataaa
     4681 atccacatta cccgcaatgg ccgcctcaag aaatttatca tcttttggat catcttcaat
     4741 aaaccgaatt ttttcttcag gtatcacaaa ctctgaaaac tgataaatgt aggttgtgat
     4801 tttatctatc gtttcctgtt tcaacccaaa tttaggtcgg ttgagaacct caaaatactc
     4861 actcaaaata tccgtggtaa caacaacaga aaatacgcca gcgtcccatt tctctaaaac
     4921 caaaaccagc gcaccaccaa gcgcgctgga aatcaaaata tttgtgtcga gaactgcgcg
     4981 tttcactgct ttccatcccg cacagcacgg atttcagctt cgatatcttc ctgggtgata
     5041 tttaattttt tcgcagttgc tcttgcgtcc tccaatgcct ccttgaaatc agaacgggct
     5101 ttttcacttt ctggcaggct gattttcaga aaccgaatga acgccagtac atccgcctga
     5161 cgagattgag ggagtgtagc aacttcacgc aaaagagttt gttcaaaggt agtcatgatt
     5221 caccttaggc ttaattatac aacttatcaa aaggggtaac tgcaaggcat tagatatcgt
     5281 ttgattataa tggaagtcac tacagaggaa accctatgaa aaaatccccc actgaaattt
     5341 ctgaagccgt tgaagtggcc cgcgaagaat tgatcgcttt tctgcaaaag ctggtgcaaa
     5401 ccgccagcct gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcccgcga
     5521 agaattgatc gcttttctgc aaaagctggt gcaaaccgcc agcctgccaa atcacgagca
     5581 cgatgtccaa aaccttgtgg cgcaaaaatt acgttcgatg ggcatggaag tggagatcat
     5641 cccttcgaat ttcgacaatc tgaaaggcca ccccgctttt ggggatgatg gcttctcgcc
     5701 aacagatcgt gtcaacgtga tcggacgctg gcccgggact cgcactggcg aaggaaaatc
     5761 gatcatcctt aacggacacg tggatgtggt tccagtgggc gatctctccc tttgggtgga
     5821 ttcgccctgg agcggcgtta tccgcgacgg aaaaatgtac gggcgcggct cctgtgacat
     5881 gaaatcgggt ctgtgcgcgg gtatctttgc agtggacgtg ttgcaaaaac tcggttacca
     5941 gcctgccaac gatattctga tcgaaagtgt cattggcgaa gaatcgggcg gcattggcac
     6001 attgaccacc attgtcaaag gttacacggc tgacgctgtg atcctgcttg agcccacgcg
     6061 gctggagctt tctccagtgc aatcgggcgc actgacattc cgcctgaccg tgcctgggcg
     6121 ttcgatccat gcggcgatga aggcttacgg tgtgagcgcc atcgaaaaat ttgccttgct
     6181 gttgaatgtc atcaacaaac tcgatgcaga acgccatgcc agcttcaagc atccattgtt
     6241 tgaagatccg cacaacattg cgccgatcaa cattggcaca gtacgcggcg gcgagtggca
     6301 ttccaccgtc cctgaagaag tggtggcaga gggacgtatg ggggtgttcc ctggggaatc
     6361 gaatgaagcg gcgcggaaaa tgttcgaagc ggcgattcag accgcagcgg agtctgaccc
     6421 gtggttgagt caaaatccgc ccagggtgga atggttcgag ggtcaattcg aatcgggtga
     6481 aactccgctt gaacatccgt tgattcaaaa tctcgctgcc atgcatgaac aggtcacggg
     6541 gtcggcggca actctgcgtg gtgtaaccta cggctcagat atgcgcctgt tcaccaacca
     6601 tgcgaaaatt gcagccacac attatgggcc tggtgacgta ggcatggcac atgcggtcaa
     6661 tgagtttgtg ccgctcgatg aggtggtgac tgcggcgaag gtcgtggcaa atacagtcgc
     6721 acagtggtgc ggaggagaat tcaaatgaaa acatttggtt gtcagtccat gtatgcgcca
     6781 attcgacgga tggtattgaa acatccgcgt gaggctttta tttcgcaggc aaatcttgaa
     6841 gcgcagtggg aaaagctggt gtattttggc tgccccgatt ttgaaaattc gctggcagaa
     6901 tacgaacgat ttacaggcat actggagcat ttcgattttg agatccacta cgcgccgatg
     6961 gatgcaagca ccagcctcga ttcaatttac acccacgacc cgctggtaat ttcgaatcgc
     7021 ggcgcgatcc tgtgcagcat gggcaaggag gcacgcaaac cagaagccga tgccgcagaa
     7081 aaatatttac atgaattggg cgtgcccatc cttgggcgga ttcacggaag tggaaaattg
     7141 gagggcggcg atgtgctctg ggtggacgaa cgcacactgg cagtgggaca gggctttcgc
     7201 accaatgcag agggcttacg tcaattgcgt gaactgctca gcgatgccgt ggatgaaatt
     7261 taccccgtac aactccccta ctggacgggg ccgcaagatt gtttgcatct gctgtctttc
     7321 atcagcatcg tggataagga tctggctgtc gtacattcgc gcctgatgcc cgtgcccttc
     7381 cgcgaatggc tgctggaacg cggctttcga tttgtggaag tacccaatga agaatacgac
     7441 aaccttggct gcaatgtgct ggcagtgggg ccgcgtcagt gtgtgatgtt gagcggcaat
     7501 ccgatcacac aaaaacggct tgaagaagct ggggcgcaag tatggacctt cgatggaaaa
     7561 gatatttcct tcaaaggcac aggcggtccc acctgcctga cgcgcccgat actgagaagt
     7621 taaaaaacaa cggcggccca gaagccgccg ttgttttttt gtcttttgca actcacctag
     7681 aaagatgcaa ccgccttcaa caagtctgta tgggattcga tgtacatatc gaacccgccc
     7741 aaattgaaag ccttggcggc atcggaggaa agattcaaca ccttgatgta gggtgatttg
     7801 tatgttcccg cactgatgcc tttacggatc tcttcatcat ctttatcggg tttggcggcg
     7861 cgcaactggt tgtaaatcga atgaatggtg cgcaggcccg cactgctgat aaagggggaa
     7921 tttgtcaggt caagcaaaat atactgggca ccacttttga tcaattcatt agccttgctt
     7981 tgaaacgcct cataagtagc tgagtttagt tcgccatgca catgcataac ctgaaccggg
     8041 acgcgtccct tcattgttgt gatcttgatt tccatgcaat cctccgatgc aaaatttggc
     8101 cgatttcttt gtgtagttta acgggtttcg gcctgtctcc cgaaatcgaa acgaatttgt
     8161 gatttactga attcaacaac tattttgaag tggaagaggt ctgctgcttg aaaccaacca
     8221 atttgcggag gatatcaaac cagaacggcg caccctgcgc cgccgcaaat gcggtgagga
     8281 ggtagccaaa aatcttcacc atgagacccc agccattatt gaaagcaggc aggcttgtaa
     8341 cagcaagaca attcccttca gagctgatca cgacacgatt gtccatgaag cttaccaaac
     8401 cacaggaagt cccttctgcc agcggggtcg tcgtccaccc aactggaaag accaaccctt
     8461 tgacattatc cagtgatatg gaatcagcag acgaatcgcc ttgcgcctcg gtctgagcct
     8521 gcgcaacaat cacggcccgc gccgtgggtt cctgccataa tttttgagca ataaaaatgg
     8581 tgtccacatt gaaaaataca gccagggcaa ggccgatcca aaaggctttc cattgggaat
     8641 cgatcttgta ccaaccgctc gcctgcacca tgacattatc aaaccagcct tcgatatttc
     8701 ggcggtattc ggcaagcttg tcgtcaaatt ccttcgtgtt gcgttgaagc gtttccatgc
     8761 ttttttccat atttggaaaa acatagggaa gcacctcgtt ctttgccatc agtgtcttga
     8821 aattggcggt catatcagga atcgacaggg tctcggcagc gatatctttt tcacctttgc
     8881 ccgcgttcat caaaacctca aacaccgccg tggcaaacac ctgcgttgga atgtaatccg
     8941 ggcggcgggg ttttcccttt ttatccagtt tcaggttccc atcattgtcc ttcagcatca
     9001 attcaacgat cagtggatgg tcatagaaac gatccaccag ggtcttatct ttcaacatgc
     9061 tatgaatgga tttttcgaga tgtttggcac gtccattcat ccaaacacca agcctgttct
     9121 gggcctccat ggtggtaatg ctaatgacca gccacacgaa cacaagcccg atggcaactt
     9181 cgataatggc gtctaattgc atgatggtct ccttgggtct gatggttaca cgccttacag
     9241 caccagtata taagcggaaa ggcttctttt caaggtcagg tcaagcgttg ggaagttctt
     9301 cctgagcttg caaatgtgat tgattgacaa gataaattcc aaccagtgca atcgccccgc
     9361 ccacccactg aatcgtgctc ggaacttctc ccaaaagcgg aatcgccagc acagccgtta
     9421 aaacgggttg ccccaccatt gtaggcgaaa ccacataggc tggcaaatgg ccgagtgcgt
     9481 acgtgatcgc cagatacccg atcatctgag aaacgacagc cgtggcaagg aagatgagcc
     9541 atgatttcaa cgggtaatcg gtgagctggt ttcccaacac aaggttcatg atgaacatgc
     9601 cgatcacagc gctcaccccc accagccagg tatagcgcaa cggatcaatg tgctttcgtc
     9661 cgcgctgagt gaccagttga tacgaagcat aaaaaacggc ggcggtgctc gccatcagat
     9721 cgccgaggct gagggtgggg tgcttcaaat agtcactgcc catcacaagc acggcacccg
     9781 tcaatgcaat gacaagtccc acccaaaatg agcggcgtaa tttttcgtga agcacgaaaa
     9841 atgcgaacaa ggccacccac aacggcgaag tattaccaag caaggttgca ttggcggcag
     9901 tggtgaattt caaagatgaa ttccagaatc caaaatcgaa agcggtgaaa agcccgccca
     9961 aaagcggaaa gagcagatat tttttctcaa tgggttccag attcttttgc tgacgaatga
    10021 aaagcggcga aagaaagatc gtggaaaaca acagacggta aaaaccagtc accgcgcctg
    10081 gggcttcggc ccaacgtacg aacatggcag aaagactgag cgcgctgatc cccaccgcca
    10141 gcgcgagata tggaaggatt ttattttgca ttaaagaatt gtactctgtc aggcacgggt
    10201 gatggagatt ctctcaaggg tcggcaaaag ctccttgagg ctgcgcacgc taaagtcagg
    10261 tttgaagcgc tgcatttcat ccgctttaaa ttgggcgcgg cgcgtgatcc agatcgattt
    10321 catgttcaaa ttctttgcac cctgaatatc ggcctccaga ctatcgccca ccatcaccgc
    10381 ctcttcggga gaaatattcc attgtgccag tgcgatctca aatgcacgcg gatgcggctt
    10441 gcgataaaaa caggcagcgg aggtcaacac aaaatcaaag tatgaacgga tgccaaaatc
    10501 ttcgatcaat tcctgcacat cctgatcgtc gcccgcgttg gaaaaaatgc caagctgata
    10561 ttgattggaa cgaagcgcct ggatcgtatc cagcgaatca tcttcaagca tccagttgga
    10621 ttgtgtgacc gaatacaacg catccaacgc ggacctgagg actggttcgg cgacatcgac
    10681 atatcccaag tccttgagca attcccgcag aacgaagtga tacgtggtct cttgaaaatc
    10741 ctgatcgcgc tggtcgtagt attcatgcag gcgcgcccga aagacacggg tatcgagttc
    10801 gatctcatgc tggcgcaggg aatcggccag tgcctgatcg gcgcgttcta aaatgggagt
    10861 ccaatcgccg cgtgaatgca tgagagtccc gcccaggtca aagagaatat ggtgaacaga
    10921 agatgctttc attgtatttg ccctactcaa aaaaacagtg aactaccttt ctacactgaa
    10981 gcaaaaaacg gagccaatcc gcgcagaacg gcaaaaatga atccgaggtt gtttgccatc
    11041 agcgccagta taaagagggt caggatcgct tcaaaccatg gcctgcccgc cagccctgca
    11101 tatgccagcg caagaaagaa caccagcaga tacgtccagt acagggcgta caggaacagt
    11161 tccgtgccgt aattcatgtg caggatgaaa ttgaaggcca aactgcccag caagccaagc
    11221 ataagcgaaa gatgcgccga cgaacgtaac cctttcataa aaaatatgaa agcccccgcc
    11281 agcagcgcca gccacaaggc cagcgggata ttggcaaacc ctttgtagga ggcgtaggca
    11341 ttgttgggcg catcgaaggt tttcaggtca atggtggggc gcggatcttt ttccgcgatg
    11401 acttcgatgg ggctgggcgc ggtcacgccg tacaaaacca ttgaacggcc caccagtttg
    11461 aagcgctcaa gcagccgctc ggtcggcgat tcgtacacgg gtttgacaaa atttccctcg
    11521 aaagccagat ccgcggggac gaagaaaaag gtctgccagc gcgggtaaac catgctggtg
    11581 aaggcagtca acacaatgct cacggccagc accatgatgc cgtagcggat caaacgccga
    11641 aacccaaact tgctgcaaaa taagccgatc attgcctgcg caatgttggt gaccgtaacc
    11701 ccaaaaacca gcaccccggc agggacaagg acggcaaaac ggttttcctg ggcctgaatc
    11761 aaaagccaga aaaagatcaa cgaagtgaca ccaaagatgt aggtatcggt cagagacccg
    11821 aagaacaaat gcgcggcggt cgaacccagc atcaccgcaa aaataaaggc ataactgctt
    11881 ttgccaacgg cgcgtttcac gaacaaccag gccatgaaca cacacaaccc actcaacatg
    11941 gccgccacaa ggatcggcgc aagctgccat gcatcgccca tgaaaatggc aaagacacgc
    12001 accagatagc ggatggtcgt caatgccagc ggatgcacgg aacggttcaa tgcatcgcct
    12061 gtggggctgc cgaggatgaa cagccacggc cccgcatcag actcaaaaag gacggtattc
    12121 acactcaacg cggggtgatt caagatccgc gcgatgacaa ggttgatgag gaaaaacatg
    12181 ccgcctgcat acaccccagg cagattcggc ttgatgaatt caaacagcct ggttttttcc
    12241 aatcggttgt gaaatccacg agtctcgaac tgctccaaca gacccaaccc ccacacaccc
    12301 gtcatcaaag ccgctgaaag gaataaaccg aaccactccc gcggaaccat gagatgttgc
    12361 cgcaaaagca tgggcggaaa ttgcatcccc accgcgatga ccgccgccaa aaaggcaggc
    12421 aatgccaatg ccagcagcaa attcaaaggc tggcgtaaaa aaccaccgcg gaacgaatcc
    12481 gctgcacgga tgaagagata attccctgcc acactgccgg cacccaccaa aatgaccgtg
    12541 aataccaaca cgttgaagat cgaatggagc gcacccgcca aaaagccgac cgcgccatat
    12601 gcaaaaagca gggaaagcaa aaaaccgaag gaatatcccc aaaggcggtt acgcatccgc
    12661 tccaccatgg gagccgcggg caacatcaaa gcaaagaggg ctagcgcgaa ggccaatgtc
    12721 ccaaggtaat aaacgcttga aactgcgaac ggcaaaacca ccgctgccgc gccgatgcct
    12781 gccagcccgc ccagtaaaaa caggacggcg ggccttcgtt gtcgaatcca ctgccacagg
    12841 gttggaaatg cctggaatag caaaaaacca attgccagtg tgggaaaaga aacgagcgca
    12901 gcgcggtcca gagccgtttc gtagcctgag taatcaaaaa aaatgatcgc cgaagagaag
    12961 gtcagggcaa aagcgatgga caggctaaat cccacacgga acagaaactg aaagactttc
    13021 acttggtact tctcccaaac gaaaccgcag atacaaccat aagatgaccg tattttaccc
    13081 gatgaccaca ccccctaatt tggcatggat tcataatggg caaaaattga cttgaccgtt
    13141 aaattagaca tataatgaat cacatcggca cacttcaaac gctcggaaga gaaaaaggga
    13201 aaaacctatg tctgggttga tcggtcaaag tttaggacgt tatcaaattg tcgagctcat
    13261 cggcgaaggc ggcatggcca ccgtgtacaa ggcgcgtgat gcccgcctcg accgtgatgt
    13321 cgcgatcaaa ttcattcgac ctgaagccgt gcctcccgat caattcgaga tcattctcaa
    13381 acgtttcgag cgcgaagcca agtcacttgc agggctttcg caccccaaca tcgtcggcgt
    13441 gatcgattac ggcgaacatg atggcctgcc gtatctggtc atggtgtatc tgccaggggg
    13501 cacactcaag gaacggctcg gcgcacccat gtactggcgc gatgccgtcc gcttcatcat
    13561 ccctgtggcc cgcgccctcg aatacaccca cgaacacagc atcatcaacc gtgatgtcaa
    13621 accatccaac atcctgctca ccgaaaaagg tcagcccatg ctcaccgatt ttggcctggt
    13681 gaagatcttt gaaggcaaaa acgccacaca cctcaccgcc tccggagcag ggttgggtac
    13741 gccagattac atggccccag aacaatggat cggtgaagcc accgccgcat ccgaccagta
    13801 ttcgctcggt gtggtgctct acgaaatgat cacaggccac aggccctata tttcagacac
    13861 ccctgctggc attctgctca aacagatcaa cgacccgctg cccttcccca aaacatacat
    13921 cccagacctg ccgcaaagcg tggaatcggt gctgatgaag gtgctcgccc gaaaacccac
    13981 cgaccgctac gccgacatgc acgccttcgc aaacgaactg caaaacctgc tcgacgggcg
    14041 cgaagtcact gcgactggga tcaaaaccaa acaactccgc gagcaaatga caggcaagat
    14101 cgaaaagccc aagattgagc aaaagccagc cgaagaagaa aaagcaccca aaaaaacaca
    14161 tcttcaagcg gatcaaaaac ccgcttcgcc tgccgaccct cagcctgcca gccccaaaaa
    14221 gaaaatctcc tccccgtggc ttgcggttct gggcgggttt gtgctgcttg gtttgtgcgg
    14281 agcctgctgg ctgctcaacg ccagattcaa ctttttcggc ggaaccacca accccacagc
    14341 cacccaatct gtaaccctcg ccacagacgt acccaccgaa gaagttgtcg aggaaattct
    14401 ccccaccgaa accagccagc ctgccagcga accgaccgaa gcccccgcca ccgaaatctc
    14461 cctgcccgcc gaaatcaccg acgggaagaa tatctccatg cgcctagtcc ccgccgggga
    14521 attcatcatg ggcaatgacg atggcaatac cgaagaaagc cctgccagca aggtccaggt
    14581 cgctgccttt tacatggaca aatacgaagt caccaatgac atgtacgccg aatgtgtggc
    14641 agcaggtgta tgcaaactgc ccgaaaaacc agggtctgcc acgcgctctt cgtattacaa
    14701 caacccatcc tttgcaaatt atcccgtgct ctacgtcaac tggaccatgg caaagaccta
    14761 ctgcgaatgg cgggatgccc gcctgcccac cgaagcagaa tgggaaaagg ccgcccgcgg
    14821 catggatgat caacgcgcct acccatgggg taacgacttc aactgtaact ttgccaacca
    14881 ttcaggttgt gttggcgata cagcccccgt caaccaatac aacagcggac aaagccccta
    14941 cggcatttac ggcctctcgg gcaacgtctg ggagtggacc agcagcctgt ttgccttcta
    15001 cccctacagc gccaccgatg ggcgtgaaga cccaactgca acaggtttcc gttccgttcg
    15061 cggcgggtcg tggaatatct ttggcggctc aggcggaaac atccgcattg acacgcgcta
    15121 caaactcgac cccgtttact tcggcgccta cgtcggcttc cgctgcgcac tgacaaaata
    15181 gcagcaacac aacagccgcc caaaccaatc ccatttgacg accgatcgca ggtgcgattt
    15241 gtcgtcattt tggttaacac gcagacaaag tctgtcggtc atttcgctca agccgccgtc
    15301 ctcggcggcg ttctacgaat aaatcctccg ccgaggacgg cgaagggagc aactcatcat
    15361 tttggttaac gcatggtcac aatgtagtgg gaaacgcaca acaagtcgct tataatagcg
    15421 gcattcaacc cgcttaaaat ttgtattttc atccgaggaa acctttatgc cgttgctgat
    15481 cggtcaaaca tttgggcgtt atcaaatcct tgagctgctt ggagaagggg gcatggccac
    15541 tgtctacaag gcatacgaca cccatcttga gcgtgaagta gccatcaagg tgatccgccg
    15601 cgatgccttc ccccccgacg acatgaacat gctgctcaag cgttttgagc gtgaagccaa
    15661 atcactgggc aggctttcgc atcccaacat cgttggggtg attgactatg gcgaacacga
    15721 tggttctcca tatcttgtca tggtgtacct gcccggcggc acactccgtg aacggcttgg
    15781 aaaacccatc gcctggagat ccgcagtgga attgatcctg cccattgccc acgccctcga
    15841 atacgtccac gaccgcaaca tcatcaatcg cgatgttaaa ccctccaaca tcctcatgac
    15901 cgaaaaaggc cagcccatgc tcaccgactt tgggttggta aaacttttcg gcgaacagga
    15961 aaaagaatca acccacctca ccagttcagg caccggtctg ggcacacccg attacatggc
    16021 ccccgaacag tggacaggtg aagccattcc acaatcggac ttgtattcgc ttggcgttgt
    16081 actctacgaa atgatcaccg ggcgcaaacc cttcaccgcc gatacccctg caggcgtttt
    16141 gctcaagcaa gccaccgaag ccctgcccct gccgcgggcc tacatcccag acctgccgca
    16201 ggaggttgaa tcggttctgc tcaaagtcct tgccaaagac cctgaagatc gctaccccaa
    16261 catgcacacc ttcatcggtg aattgcaaaa cctgctcgct ggcagggaag tctctgccac
    16321 ttcgatcaaa gcggaccagc ttcgcaaaaa gatgaccggc aggaccgaag cgaccacatt
    16381 tgacccggca acgggtcgga cccaaaaggc attccccacc ctttttgccg tcctcggcgg
    16441 gttgggcgca ttgatggtca ttgcggtcat cggcgtattg atctacgctg ggaacggcgg
    16501 caccgccctg cctttccttg ccgcacccac ccaaacctca caaccgaccg caattccctc
    16561 cgctaccaat acagccattc cgccgacaga aacggtcgag gtcactgccg ccccgacgga
    16621 aacgcttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt cttcaaggat
    16741 taccccgtca tctacgtgga ttggaaatgg gccaaagcct attgcgagtg gagaggggca
    16801 cgcctgccca cggaagccga atgggaaaaa gctgcccgcg gaactgatgg tcgtctttat
    16861 ccatggggta atgaaaagcc cgattgcaac cttgccaata ttgcaggctg cgtgggtgat
    16921 acaaccccgg tagaccaaca tgaactgggg aaaagcgtgt atggcgttta tggaatggct
    16981 ggcaatgtga tggaatggac cagctcactg ttcgactatt atccctacga tgccgctgat
    17041 gggcgggaaa accctgactc taccaaagag cgcattgtcc gcggcggcgc atggaatctc
    17101 ttcggcggca acagcggaaa cgtccgcgtg gatacacggg tgaagttatt ccctgaatct
    17161 gccgtagcct acgtcggcat ccgctgtgcg aaatcggcac aataaacaga agaactcgga
    17221 accgtaaaag ttccgagttc tttttatttt tttatttcca acaacacggg ctggatcagc
    17281 taaaaatccc aggtgcgcgc cactcgtaca gcacttcgag attgcccttc aggaaggatt
    17341 ctcgtgtggc atttctttga atttttccac tcgtggtctt ctggatggtt gatggcatga
    17401 tcaatacaac cgcctgcaca cgcagaccat gattcttcgc caccgccata cgaatggcat
    17461 tggcaattcc ctcaaaatca ttgctcttgc gatactcgcg ttttacttcc tgcacgacca
    17521 caagatgatc cacctgattc gcctcgaccg caaaggcggc gccaaagctg gggcgcaggg
    17581 catcatggca tttttccatc gtcaactcaa tatcctgcgg ataaaaatta cgcccttgaa
    17641 taatgaccat atccttgatg cggccagtaa tgtggatctg gtcgttgtag ataaaaccaa
    17701 ggtcacctgt ccgcatgaac gggccttcgt tggacgatgc aatataagcc tgaaatgtct
    17761 tttcggtttc ttcggggcgc ttccaatatc cctgtgccac actggtgctg ctgacccaga
    17821 tctcgccaac ctcgtctttc gcacacaatt cctttgtctg cgcgttcacg attcggatat
    17881 ctgcattaat ggaactccat ccgcagccca cgct
//
LOCUS       DAOQJC010000085        17638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1519_length_17638_cov_10.337201, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000085 DAOQJC010000000
VERSION     DAOQJC010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 17638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17638
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1519_length_17638_cov_10.337201"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..925)
                     /gene="era"
                     /locus_tag="PLE39_12940"
     CDS             complement(23..925)
                     /gene="era"
                     /locus_tag="PLE39_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNE69282.1"
                     /translation="MTNHRSGFVAIIGRPNVGKSTLLNALLGQKVAAVSPRPQTTRKR
                     QLGILTIENAQIVFVDTPGIHNPKHMLGEFLNQEAQEALDGVDLILWLVDSSTSPTEE
                     DIQIADILSKAARRTPRMLVLNKIDMVGADDESARGEEYARLLNQATVMKVSAARRLG
                     LGEMLETLIQLLPERPPEYPEDQVTDLYEREIAGDLIREACLIRLRDEVPHGLAVRID
                     EFKERENGMAYIAATIFVERESQKGIVIGEGGRMMKTIGSQARKEIEEMGGRKVFLEL
                     RVKVSKDWRNDPNALKQLGYAKKK"
     gene            complement(922..1800)
                     /locus_tag="PLE39_12945"
     CDS             complement(922..1800)
                     /locus_tag="PLE39_12945"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNE69283.1"
                     /translation="MKTKHWIAFIALGVIWSSSFLWIKIGVQEVSPMVLVAFRMLFGA
                     LAASSVVIYQKLEWPRDWKTWGIYAILGPTSLAIPIFFISWGEQSIDSAVASILNATV
                     PLFTLIIAHFYLHDDRMTSQKVIGLLVGFAGIVILLSKDLTNSAHNSVIGQAAVILAS
                     LFYAGSAVWARKVTAHVQGAVRGAAPLITATIFMWILLPFMENPIKFPALPITWVAAL
                     WLGVLGSGFAMILNYYLLHEIGPTRATMVTYIFPPGGVILGVLFLDESLSWQLFAGAL
                     LIIASLAIVNWKKKES"
     gene            complement(1909..2784)
                     /locus_tag="PLE39_12950"
     CDS             complement(1909..2784)
                     /locus_tag="PLE39_12950"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE69284.1"
                     /translation="MKTKHWFVFITLGLIWSSSFMWIEIALREVGPLTLVAFRAFFGL
                     LFGLVVLFIQKVPFPRTWKEWAPLIALGMTNIALPFFLISWGQKTIDSGVASILDATV
                     PLFTILVAHFLVHDDKMTAQKVIGLLLGFFGVLVILIKDIGSGTNSITGEFAVILACV
                     FYAASTIIARKFTEDMPAIYRSMGPLAAATVVMIMAAAAFEAPFKIPALPLTWIALAW
                     LGILGSGLAFLIVFYLIHEIGPTRSTMVTYLMPLGGVTLGVVFLNEPLTWQMIVGAVL
                     IILSLAVANWQPKQA"
     assembly_gap    3257..3356
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3528..4409
                     /locus_tag="PLE39_12955"
     CDS             3528..4409
                     /locus_tag="PLE39_12955"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNE69285.1"
                     /translation="MELNHLKTFVVVAEERNLTRAADRLYMTPPSVSAHIKALEDELG
                     VILFIRKPQGMEITEHGEILRVKAEKILHSTREMLNLSKKLQVSLTGTTRIGLSATSS
                     YQRAAKLISQMQKEHPDIELTFLASCTHKIIQGLKNHSMDAGYMFGPIDDEALAGHEL
                     DIADLVIIAPSTWKDKVAGADWKEIAALPWVGSSYYCPFQTALEKLFKKRKLAMNHVV
                     WMDDEFNKCALVEEGIGMALLEKSEAEQASSNGRIFIWKTEPIKCKVSWVHLRNRSDD
                     PLIAALTAQVLNVWGKA"
     gene            4406..4864
                     /locus_tag="PLE39_12960"
     CDS             4406..4864
                     /locus_tag="PLE39_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69286.1"
                     /translation="MNSYLIRQAEPKDLPEIIQLCAEHAAFEQADYSPSGKADKLSAH
                     LFNNPPTCFCLVVEMDGALAGYATYSKEFSTWDADFYIHMDCLFLRPHARSMGIGEQL
                     IKQIAQSAQALNCRLIQWQTPKFNARAVKFYKRIGATEKEKLRFFLTVDA"
     gene            4961..6217
                     /locus_tag="PLE39_12965"
     CDS             4961..6217
                     /locus_tag="PLE39_12965"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE69287.1"
                     /translation="MEETNKNWAVRFYTLWTGQALSLLGSQLVQFAIIWYLTQTTNSA
                     TTLATASMMGLLPQVLLSPFIGTWVDRGNRRMILIFSDAIVALATLGLVILFATGQIQ
                     IWHIYIALFIRAVGGGFHQSAFGASVVLLVPKDQLARVQGFNQALNGGLNIISAPLGA
                     FLLSILPMQGILSIDVSTALLAISIVFFTHIPQPERGEKKPSTFWQDFREGFRYIMAW
                     RGLVILLGLVMVINFFFSAIEPLTPLLITKHFKGNAAQLGWWLSLFSMGVLLGGLLLG
                     IWGGFKRKIITAQGGLVLMGLFAAIIGFLPENMLWAGMVINMLFGLTQPIVNGSYGAT
                     LQAAIAPDMQGRVFSFIFSAAMLVSPLALMISGPFADRFGIQIMFVIAGVTCMLLGAA
                     GFFITDVMEFENSSKEKSFHATVDLS"
     gene            6286..6690
                     /locus_tag="PLE39_12970"
     CDS             6286..6690
                     /locus_tag="PLE39_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69288.1"
                     /translation="MERKPALEPELVNEFVGKAHGDLERVKEMLENEPALVNATWDWG
                     GGDFETALGAASHMGRKDIAIFLLEHGARLDIFAAAMLGKLDIVKAVLTAYPNLLDTP
                     GPHGIPLLTHAEIGGQDAAAVSDYLQSLKRSS"
     gene            complement(6674..8335)
                     /locus_tag="PLE39_12975"
     CDS             complement(6674..8335)
                     /locus_tag="PLE39_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HNE69289.1"
                     /translation="MEFTEKRQHWKKINALTPQKLELAHRLLEKIREGGDVMVLLRRH
                     PLEGGGYLNKAALVAAYNEMVAAGTIEPDGALLERIRMKPMRTLSGVTTVTVLTKPYP
                     CPGKCIFCPTDVRMPKSYLPDEPGAMRALEHQFDPFTQVESRIRALKNLGHPTDKIEL
                     LILGGTWSSYRRDYQEWFVKRCFDAMNNTNHGGMEPQREHEATLRSDAPVVNLSEIHS
                     QNENAPHRNVGLVIETRPDEINADEIQWLRHLGVTKVQMGAQSFNDHILDINKRGHDV
                     ESTRRAVALLRAAGFKIVLHWMPNLLGATPQSDREDFARMWEDFCPDEIKIYPNQLLA
                     NAELYEYWQRGEFHPYSTEELVDLIADIKPTIPRYCRVNRVIRDIPGNNVVEGNTRTS
                     LRQDVQEEMKKRGTRCQCIRCREVKGKAVELSSLQLNDFVYQAGRAEEHFVSFNTADD
                     GLAGFIRLSLPSKESPETGMADLNGAALIREVHVYGQSLAVGAEKTGAAQHVGLGTRL
                     LEEAEAIARANGFEKLAVIAAVGTRQYYLDRGFERGEYYLVRKIV"
     gene            complement(8369..9127)
                     /locus_tag="PLE39_12980"
     CDS             complement(8369..9127)
                     /locus_tag="PLE39_12980"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNE69290.1"
                     /translation="MLSAYRIEKYSQEFETKTPQEIIAWAVDRFSPYLAMSSSFQTQS
                     MPLLHMATRIHPEIPIFFIDTGYHLWETLMFREQLATEWNLKIVDLYRDSRWDVFVRQ
                     HARTLPLEDPNLCCYLHKVQPMQTALHGYKAWISGIRRDQTAVRAQAKFLELQEDGLL
                     KINPLLNWTKADVNRYIEENNLPKHPLYEKGYRSVGCAPCTIAIGVTDDERAGRWVGR
                     GKVECGLHTDMFSKKDIAEVRQDFQTDVEKVNQR"
     gene            9177..9887
                     /locus_tag="PLE39_12985"
     CDS             9177..9887
                     /locus_tag="PLE39_12985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE69291.1"
                     /translation="MNSVTAARLIELNREFYDHFGDSFSATRQRLQPGVKKILDTIQG
                     DVSVLDLGCGNGHFLHELIRRGHKAPLLGMDFSLPLLRNAESAPGVEFREADLTKPSV
                     FISSIPSPWSMVTSFATLHHIPSNEIRLEILRTVKKLLKPDGRFVLSNWQFLNSEKLT
                     ARIQPWTRVGLSDSDVDEGDYLLDWRSGGEGLRYAHHFSAEELHGLAGQAGFKVESEF
                     LSDGENGRLGLYQIWAPD"
     gene            complement(9889..12993)
                     /locus_tag="PLE39_12990"
     CDS             complement(9889..12993)
                     /locus_tag="PLE39_12990"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNE69292.1"
                     /translation="MQNRTEVYVSLIYILAAIPYVWLGLHAWRKRPAVAVTPFAWVML
                     GMSIWSFGYGLELFFPYLSFKLFFAKVEYIGIVSIPVLLLFFALEFTGKSHVLTPRLR
                     LLLWIFPFMMLVLVWTNEFHQLVWSMAKVVEIRNLHLLDVRFGLFFWVHIVFANLLTV
                     FASILLVRELAQRPRSDRVQIGLVILGIFLPLIGSLLFVFGLNPIPNFDITPLLFLPT
                     GLGLSWAIVRYRLQDTLPLEHLSVLNNMKDGVIVLNSKQRVLYLNPIAEKLLGCGEDT
                     AIGQPLAHVSALYWGILRPHLTAEDARAEVVIGEGRQTTVFDVTVSPIIHKDQQQRTF
                     SPDCIITLHDITERKEMENALSRREAIMSAVSMSAEQFLKEGYWGANVLAVLEKVGQA
                     VNVSRVYVAMNYKDEKNTLYSSMCYEWVSPEVVSQINNPALRHIPLQQAGLQRWEKQL
                     SQHLSIQGLVEDFPESEHAVFELLGSLSVAAMPIFVENEWWGFVVFDECRTRRHWTGM
                     ELKTLHTIASILGSAESRTRAEQKLIRRQNTLTLLHQIVQEALQADNLKAMAEDLVGR
                     LTGLISADDCYITVWDEAASQTSPLASFIASRNTFIPLQPVFGEFTLTELALNYKTTL
                     IVEDVANSPYFDREIAEQCPAQSLLAIPLVAGKNRLGAIILTFNLYHRFQAEEIEICT
                     QASNLIALAFEKFKAMEYAQRRAVTSETLRKASAVVSGTLEMDEAVNHILEQLNRVVP
                     YDSASIQLLDGNELEIVGGHGWANVENIKGMRFEIPGDNPNSVVIETKKPYILADVRK
                     AHKAFSQAPHDHIRSWLGVPLVVRDRVIGLLSIDSAEENHFNSNDIELAVTFANQVSI
                     TLENARIFKETQDQAIIDPLTTIYNRRGLYLLGESKFEDVIFGRKEFSCIMIDIDHFK
                     KINDTHGHDAGDRVLVTLASFCKKCVREIDIVGRYGGEEIVILLPETSLEVGMMVAER
                     LRAMIAGSPVKIGENFTLSVTASLGVACKDENTTSLDILIKRADQAMYIAKNKGRNRV
                     ATSR"
     gene            complement(13095..14084)
                     /locus_tag="PLE39_12995"
     CDS             complement(13095..14084)
                     /locus_tag="PLE39_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69293.1"
                     /translation="MTDFFSAFSKLFKSSQPKYPPDSATEPAQITQSKVLVIVFDPVM
                     DKSTGVTLSQQQKWYRPADLITGFMADLLQVSGGMARYQIVQRVDVDEFPAKTDGFRY
                     TPQTYLDVLHGVTSPHVPQEVNYNAILTNHNVLQRVARGEIDEVWTFGFPHAGFYEST
                     MGGPGAFWCNAPTLKNTEASKRRFVVMGFSYERYIGEMHESYGHRAESIMEKTFSKLT
                     GPANLWKRFIQYDKVTPGKAACGNIHFAPNSQRDYDWNNPTPVPSECYDWLLNFPNFK
                     GDVRTVGPSEWGGGDIRAHHKWWFNHFPRVAGRKNGIHNNWWQYVVNPQQVIL"
     gene            complement(14124..16574)
                     /locus_tag="PLE39_13000"
     CDS             complement(14124..16574)
                     /locus_tag="PLE39_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69294.1"
                     /translation="MPLDLTLAPIYRVNGQEITSLPGLIALNPPRTAARGREKDRLIV
                     YLLLTGNAVFSTSEYMKVAQDAAAAYYQSAGSLTSSLKAASEHVNKTLLERNMSSSAR
                     GQYAIAWLTLAALRDTQCTLSMSGPMHAYWFGHEDTRHIHEPAVSGKGLGASQTINIH
                     YAQLNLNAGDRLLFVGRAPDAWDSTLNDPAPSSLDAMRRRLTTLTSVDVNAVLMQATN
                     GSGNVKLLDGTLERKKEETAAPLPTPSLPQAEETVSAPQPVEAPTEDLNYEEKPENAA
                     HVVQPSAYAIPPQRTETEPANKILASLPRNTVPRDFPASIPRIQPSQQSAEVNLPPAM
                     VEEALPIETEEEKSVEEKVIAPVKAAEPETPREPSEMTRQTAKVLATGIQSTRRINQT
                     IGEKLRNFLPRLLPNSEAGDSFAPSNFSMFAIAVGVPLMVVAMLFVVYVQYGRTPQYE
                     IFLGQAREMSTQAAMLTNPIEQRESWKNVLLYVDKAEDIYRPTSDTITLREQAEVNLD
                     KLLGITRMQFNPAFSINLGIEVSRMAASETDLFLLNAANGEALRAVPASNRRGFELDT
                     AFNCKPGVYGSYTVGPLVDILTMPTLNSINATLLGVDAAGNLLYCAPGQVAQAIPLPP
                     PDTNWGRVTAFILDSGNLYVLDSSARAVWVYTGRDGTFIDRPYFFFGGQTPEKQDVID
                     LVVSGDDLYMLHADGHLSTCSYSRIESVPTRCQDPSPLINPIAAYQDTDLFGAAHFTQ
                     VLLTALPNQSILLLDADTQGVMRFTPRSLELQSQLRPTTGTLNPIPSGPVGAIAVGPN
                     HVLYLAVGGQIYFATDMP"
     gene            16625..17638
                     /locus_tag="PLE39_13005"
     CDS             16625..17638
                     /locus_tag="PLE39_13005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69295.1"
                     /translation="MKYTLHNDFSEINSQEWNTLLSVSITDTPFLRYEYQRSWWEHRG
                     GGEWKNAVLVLVTARENEKLIGIAPLFIAEYDGRSALLLNGSIEISDYLDLIVGQDDH
                     TRFVSGLLDFLTAQSGPANWSGLDWYNLPDSSPTLAALKAESATRGWAHHEEMYRPTP
                     RIALNGSFEEYLSRVEKKQRHEIRRKVRRAEESERGVRWFISDMADVEAEIDSFISLM
                     EQDQNKASFLHPPMRAQMRDVIRTAHQNGWLWLAFLEADGQRIAAALNFDYNNKLWGY
                     NAGVNRAYMDLSPGWVLLAYILEWCCQNGRSEFDFMRGDEEYKYRFGAVNQYVMRAKL
                     TRA"
BASE COUNT         4275 a         4684 c         4459 g         4120 t
ORIGIN      
        1 gaagaaagag gaaagatgtt cgttacttct tcttcgcata ccccaactgc ttcaatgcgt
       61 tcgggtcatt gcgccagtct tttgaaacct tcacacgcaa ttccaaaaaa acttttctgc
      121 cgcccatctc ttcaatttcc ttgcgggcct ggctgccgat ggttttcatc attcggccgc
      181 cttcaccgat gacgattcct ttttgcgatt cacgttccac aaaaatggtc gcggcaatgt
      241 aagccatgcc gttttcgcgt tccttgaatt cgtcaatgcg cacagcgagt ccgtgcggca
      301 cttcgtcgcg caggcggatg aggcaggctt cacggatcag gtctcccgcg atctcgcgct
      361 cgtacaggtc ggtcacttga tcttcgggat attcaggcgg tcgctcaggg agaagttgga
      421 tcaaagtctc gagcatttcc ccaagcccaa gccttctcgc tgcggatact ttcatcaccg
      481 tggcctgatt cagcaatctg gcatattcct ccccgcgagc agattcatca tcggcgccga
      541 ccatgtctat tttgttcagc accagcatcc ggggagtccg acgcgctgct ttgctgagga
      601 tgtccgcaat ttggatatcc tcctcggttg gtgaggtgga ggaatccacc agccagagga
      661 tcaagtccac gccatccagg gcttcctgtg cttcctgatt gagaaattca ccgagcatgt
      721 gtttggggtt gtgaatgccg ggcgtatcta caaagacgat ctgggcattc tcgatggtga
      781 gaatacccag ttggcgtttg cgcgtggtct gcgggcgcgg agaaacagcg gcgacctttt
      841 gcccaagcag ggcgttgagc aaagtggact tgcccacatt ggggcgtccg atgatggcga
      901 cgaagcctga acgatggttg gtcatgattc ctttttcttc cagttgacga tggcaaggct
      961 cgcgatgatg agcaacgcac ccgcgaacaa ttgccagctt agcgattcat cgaggaacaa
     1021 cacgccaagg ataacaccac ccggcggaaa aatgtatgtc accatggtgg cgcgggtggg
     1081 gccaatttcg tgcaacaggt agtaattgag gatcatggca aagcctgaac caagcacacc
     1141 cagccacaaa gcggcgaccc aggtaatggg cagtgcaggg aatttgatgg ggttttccat
     1201 gaacgggagc aggatccaca tgaagatggt tgcagtgatg agcggagccg caccacgtac
     1261 tgctccctgc acatgagcgg tgaccttgcg cgcccacacg gcgctccccg catagaacag
     1321 cgaagccaga atcacagccg cctgcccgat gaccgagttg tgcgcgctgt tcgtcaggtc
     1381 tttgctgagc aggatgacaa tgcccgcaaa cccgaccagc aggccgatca ctttttgtga
     1441 ggtcattctg tcatcatgca ggtagaagtg ggcaatgatg agcgtgaaca gcggcacagt
     1501 ggcattcaaa atggaagcca cagccgaatc gatggattgt tctccccatg aaatgaaaaa
     1561 gatgggaatg gcaaggctgg tggggccaag gatggcgtaa atgccccagg tcttccaatc
     1621 acgcggccat tccagttttt gataaatcac cacagacgat gcggccagcg caccgaataa
     1681 catgcgaaaa gcgaccagca ccatggggct gacttcctgt acgccgatct tgatccacaa
     1741 gaaggatgaa ctccagatca cgccgagggc gatgaaggca atccaatgtt ttgttttcaa
     1801 aatatcctgc tttctgaaat tggtggtgta tgtcgtgcgt aaaccgaccg ccgacgatgg
     1861 acggcaggct gcggtcttcg gtccatgtct gatgaaattg cggatgtttt atgcctgttt
     1921 cggctgccag tttgcgactg caagactcaa tatgatcaac accgccccga cgatcatttg
     1981 ccaggtcagc ggttcattga ggaagaccac gccaagggtc acgccgccga gcggcatgag
     2041 ataggtgacc atggtggaac gggtgggtcc gatctcgtgg atgagataga acacgatcag
     2101 gaaggccaac cctgaaccta agattcccag ccatgcgagg gcgatccacg tcaggggcag
     2161 agcggggatc ttaaagggcg cttcgaacgc agcagcggcc attatcatta ccacagtggc
     2221 ggctgccaac ggccccatgc tgcggtaaat ggcgggcata tcctcggtga attttcgggc
     2281 aatgatggtg ctggcggcat agaatacaca ggcaaggatg accgcaaatt cgccagtaat
     2341 tgagttcgtt ccgctgccga tgtccttgat cagaatcacc agcactccga agaaacccaa
     2401 taatagcccg atcacttttt gagccgtcat tttgtcatca tgcacgagaa agtgcgcaac
     2461 gagaatggta aaaagaggaa cggtcgcatc caaaatggag gccacgccag aatcgatggt
     2521 tttttgtccc cagctgatca gaaagaacgg cagggcaata ttggtcatgc caagtgcgat
     2581 cagcggcgcc cattccttcc atgtgcgggg aaaaggcacc ttttgaataa aaaggaccac
     2641 caacccgaac aaaagcccaa agaaggcgcg aaaggcaaca agcgtaagcg gtcccacttc
     2701 gcgcagggca atttcgatcc acataaagga tgaactccag atcaaaccga gggtgataaa
     2761 gacaaaccag tgttttgttt tcaatgtaac tccttaaaat acgtcaggct tttttgcagc
     2821 ttgtacgtgt gaatacgaat tttataggaa ggttgtcttt atcagccacc cgattcttgc
     2881 tcttttaggg atcgatgcaa ccttccggct tataaaccct gatcttggag gctgggttct
     2941 cttttagcct ggatgccggg cggcgtggtc gctgtaccag ctcgccgccg caatttgggc
     3001 aaatgttgtt cagttttgaa tctgcgcaat gggcacaaaa ggtgcattca aaggtgcaga
     3061 tgcgcgcctc tgttgaatca ggcgggaggt caatgttgca gcattcacag ccgggtctca
     3121 tttccaacat ggattctcct ttttagagcc aaccgcgttc cacgggatat ttttctttgt
     3181 cttcccaata ttcaatgccg ccgatcatct ctttgacgta aaagccaaga gcgctgagtt
     3241 tggctgcggc tttggtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngccg
     3361 gggtggctgt aactcttggt ttgggtttaa gtgtgaccat cttttctcct tgaattggaa
     3421 tgttccaagt gtatggaaaa gatgcctgtt tgtgaatttg aggttgccaa acacagcgtt
     3481 cggtgaagcg aaacgctcgg catgacagaa tcatgtatac tgcggacatg gaactgaatc
     3541 acctgaagac atttgttgtt gtagctgaag aaagaaacct gacgcgcgca gccgacaggc
     3601 tttatatgac accgccttct gtcagcgctc acatcaaagc attggaagat gagttgggcg
     3661 tgatcctctt tatccgtaaa ccgcaaggca tggaaattac ggagcatgga gaaatcctgc
     3721 gcgttaaagc tgagaagatc ctgcactcca cacgtgagat gctcaatttg tcgaaaaagc
     3781 tgcaggtctc tctcacaggc acaacccgca tcgggttaag cgccacatca tcctatcaac
     3841 gtgccgcaaa actcatcagt cagatgcaaa aagaacatcc agatatcgag ttgacctttc
     3901 ttgcgtcctg cactcacaag atcattcagg gattgaagaa tcattccatg gatgcagggt
     3961 atatgtttgg ccccatcgac gatgaagccc tggctggaca cgaattggat attgccgacc
     4021 ttgtgatcat tgccccctca acctggaagg ataaggtggc gggggcagac tggaaagaga
     4081 tcgcggcatt gccctgggtt gggtcatctt attattgtcc cttccaaacc gcgcttgaaa
     4141 aacttttcaa aaagagaaaa ctcgccatga accatgtggt ctggatggat gatgagttca
     4201 acaagtgcgc actcgtggag gaaggcattg gcatggcatt gctcgaaaaa agtgaagcgg
     4261 aacaggcttc ttcaaacggc aggatattta tctggaaaac tgaaccgatc aagtgcaagg
     4321 tctcgtgggt gcatttacga aatcgcagcg atgacccttt gattgccgcc ctgacggctc
     4381 aagtattgaa tgtatgggga aaagcatgaa ttcgtatttg atacgacagg cagaacccaa
     4441 agacctgcca gagatcatcc agctatgcgc tgaacatgcc gcatttgaac aggcggatta
     4501 ttctccatct ggcaaagcgg ataaattatc ggcgcatctc tttaacaacc cgccaacctg
     4561 tttctgtctc gtcgttgaaa tggacggcgc cctggcaggg tacgccacct attccaagga
     4621 attctccaca tgggatgcag atttttacat ccacatggat tgcctgttcc tgcgtccgca
     4681 cgctcgcagc atgggaattg gggaacaatt gatcaagcag atcgcacagt cagcacaggc
     4741 attgaattgc cgcctcatcc agtggcagac ccccaaattc aacgcacggg cggtcaaatt
     4801 ctacaaacgc atcggcgcta ctgaaaagga aaagcttcgt tttttcctga cagtggatgc
     4861 gtaaagatca tcaacaaaac agaataacca ttctcaaggg ggttatcata aaagcgcgac
     4921 catactacca ttgagggaaa aatctcttag aggtgtcttc atggaagaaa caaacaaaaa
     4981 ttgggccgtc cgtttttata ccctgtggac agggcaggcg ctgtccctgc ttggcagcca
     5041 attggtgcag ttcgccatca tctggtacct cacgcaaacg acgaactcag ccaccaccct
     5101 cgcaactgct tcaatgatgg ggttgcttcc gcaggtgcta ctctccccct tcatcggcac
     5161 ctgggtggat cgcggcaatc gccgcatgat cctcatattt tcagatgcga tcgttgccct
     5221 tgcaacgctt ggactggtca ttttatttgc cacagggcag atccagattt ggcatattta
     5281 catcgccctg ttcatccgcg ctgtaggagg cggatttcat caatcggcgt ttggcgcatc
     5341 ggtggtattg ctcgtgccca aagatcaact tgcgcgtgtg cagggattca atcaggcact
     5401 gaacggcggt ttgaatatca tctcagcgcc gctgggggct tttctgcttt ccatactgcc
     5461 catgcagggc attctttcca ttgacgtgag cacggcgctg cttgcaattt cgatcgtctt
     5521 ctttacccac atcccacagc cggagcgtgg cgagaaaaag ccctctacct tctggcagga
     5581 ttttcgggaa ggctttcgct acatcatggc ctggcgcgga ttggtcattc tgttggggct
     5641 ggtaatggtt atcaactttt tcttcagcgc catcgaaccg ctgaccccat tgcttatcac
     5701 aaagcatttc aaaggcaatg ccgctcaact gggctggtgg ctttccctgt tttctatggg
     5761 ggtcttgctg ggcggtttgt tgctggggat ttggggaggc ttcaaacgca agatcatcac
     5821 cgcacaaggc gggttggttc taatgggttt atttgccgcc attatcggct tcctccctga
     5881 aaatatgcta tgggctggca tggtcatcaa tatgctgttt gggttaaccc aacccattgt
     5941 caacggttcg tatggcgcga ccctgcaggc agccatcgca cctgatatgc aaggcagggt
     6001 gttctccttc atctttagcg cggccatgct ggtctcaccg ctggcgctca tgatctcagg
     6061 tccatttgcc gaccgcttcg ggatccagat catgttcgtg atcgcaggag tcacttgcat
     6121 gctcctggga gccgcgggct tcttcatcac cgatgtgatg gagtttgaga attcttcaaa
     6181 ggagaaaagt tttcatgcca cggttgatct ttcttgattt tgatgcactt ggggattaag
     6241 ggtagaattt ccatccgaaa gaacatttat caacaggagc aaacaatgga acgaaaacca
     6301 gctttagaac ccgaacttgt gaatgaattt gtcggcaagg cacacggcga ccttgaacgt
     6361 gtcaaagaga tgttggaaaa tgagccagca ttggtcaatg caacatggga ttggggcggg
     6421 ggtgattttg aaaccgcgct tggtgcagct tcgcacatgg gacgcaaaga catcgccatc
     6481 tttttgctcg aacatggtgc gcgccttgat attttcgcag cagccatgct tggaaaactc
     6541 gatatcgtca aagccgtgct gacagcctac cccaacctgc tagacacccc cggtccacac
     6601 ggaattccgc tcctcaccca tgccgagatc ggcggacagg atgcggcggc agtttcggat
     6661 tatttacaat cgctcaaacg atcttcctga cgagataata ttccccgcgc tcaaacccgc
     6721 gatcaagata atattgccgc gtgccaactg ccgcaatgac ggccagtttc tcaaaaccgt
     6781 tggcgcgcgc aatggcttcc gcttcttcga gcaggcgtgt acccagccca acatgctggg
     6841 cggcgcctgt tttttctgcg cccaccgcca gtgactgacc gtacacatgc acttcgcgaa
     6901 tcaaggccgc accattgagg tcagccatgc cagtttcagg ggattccttt gaaggcagcg
     6961 aaaggcggat aaatcccgcc agcccatcat cggctgtgtt gaacgagacg aagtgttcct
     7021 ctgccctgcc agcctgatat acaaaatcat ttaattgcag cgacgataat tcgaccgcct
     7081 tgcctttcac ctcgcggcag cgaatacact gacaacgcgt gccgcgcttc ttcatctctt
     7141 cctgtacatc ctgccgcaaa cttgtgcgcg tattgccttc gacaacgtta ttccccggta
     7201 tatcgcggat gacgcgattg acacggcaat aacgcggaat ggtgggtttg atatcggcga
     7261 tcagatccac aagctcttcg gttgagtagg gatgaaactc gccgcgctgc cagtattcat
     7321 acaactcggc attggccagc aattgattcg gatagatctt gatctcatcg gggcaaaaat
     7381 cctcccacat ccgcgcgaaa tcttcgcggt cgctttgcgg ggtggcgccg agcaggttgg
     7441 gcatccaatg cagcacgatc ttgaatcccg ctgcgcgcag caatgccacc gccctgcgcg
     7501 tggattcgac atcatgtccg cgtttgttga tgtcgaggat gtgatcgttg aaactctgcg
     7561 cgcccatctg caccttggtc acgccaagat gccgcagcca ttgaatttca tcggcattga
     7621 tctcatcggg gcgggtttcg atcaccagcc ccacattgcg atgcggggca ttttcatttt
     7681 gcgagtgaat ttcagacaga ttaacaacag gcgcgtcgga acgcagggtt gcttcgtgtt
     7741 ctctctgcgg ctccatgcct ccgtggttgg tgttattcat cgcgtcgaag caacgtttga
     7801 cgaaccattc ctgataatcg cgtcggtacg atgaccacgt gccgcccagg atcaacaact
     7861 cgatcttatc ggtggggtga ccgagatttt tcaaagcgcg gattcgtgac tcaacctgtg
     7921 tgaacgggtc aaattgatgt tccagtgcgc gcattgcccc gggctcatcg ggcaaatagc
     7981 tttttggcat acgcacatcg gtggggcaaa agatgcactt ccccgggcag ggatacggct
     8041 tcgtcagcac ggtcacagtc gtcacgcccg aaagcgtgcg catgggcttc atgcggattc
     8101 gttccaacaa tgcaccatcg ggttcaatgg tgcctgctgc gaccatttcg ttatacgcgg
     8161 ccaccaacgc agccttgttc aaatatccgc cgccctccaa cggatgacgg cgcagcaaaa
     8221 ccatcacgtc accgccttcg cggatctttt ccaacaggcg atgagccaac tccagttttt
     8281 gcggagtcag cgcgttgatc tttttccagt gttgtcgttt ttcagtaaat tccatggttt
     8341 ccacgcagga gctgcccaga gacaaaaact atctctggtt caccttctct acatccgtct
     8401 gaaaatcctg ccgcacttcg gcaatatcct tcttcgagaa catatccgta tgcaggccgc
     8461 attccacttt tccgcgccca acccatcgac cggcacgttc atcgtccgtt acgccaatgg
     8521 caatcgtgca gggggcgcac cccacactgc ggtatccttt ttcatacaat ggatgtttcg
     8581 gcaggttgtt ttcttcaata taacgattca catcagcctt cgtccaattc aacagcggat
     8641 tgatcttcaa caatccatct tcctgtaatt ccaaaaactt tgcctgcgcc cgcaccgcgg
     8701 tctggtcacg gcggatgccg ctgatccagg ctttgtagcc gtgtaaggcc gtctgcatcg
     8761 gttgaacttt gtgcaaataa cagcacaggt tcgggtcttc aagcggcagg gtccgtgcgt
     8821 gctggcgcac aaacacatcc caccgcgaat cgcgatacag atccacgatc tttaaattcc
     8881 attcagtggc aagctgctca cgaaacatca aggtttccca aagatgataa cctgtgtcaa
     8941 tgaagaagat cggaatctca gggtggatac gcgtcgccat gtgcagcaac ggcatagact
     9001 gggtctggaa ggatgaactc atcgcaagat acggcgaaaa tcgatccaca gcccaggcaa
     9061 tgatctcctg cggggttttg gtctcgaatt cctgtgaata cttctcaatt cgataagccg
     9121 ataacatact gggattatac cgtttatata atgcccactc tgggtataat gccgccatga
     9181 attctgtcac tgccgcacgc ctgatcgaac ttaaccgcga attttacgac cactttggcg
     9241 attcattttc cgccacacgg caacgcctgc agcccggcgt aaaaaagatt ctcgatacga
     9301 ttcaggggga tgtctcagta ttggatctgg gatgcggcaa cgggcatttt ctccacgagt
     9361 tgattcggcg tggtcacaaa gctccgcttc tcggcatgga ctttagcctg cccctgctcc
     9421 gaaacgcaga atcagcgccg ggggtggagt ttcgagaggc tgatcttaca aagccatccg
     9481 tgttcatttc atccatccct tctccgtggt ccatggtcac ctcctttgcc accctgcacc
     9541 acatcccttc caatgaaatc cgcctcgaaa tcctgcgaac cgtgaaaaag ctgctcaagc
     9601 ccgatggcag gttcgtttta tccaactggc aatttctaaa cagcgaaaaa ctcaccgccc
     9661 gcatccaacc gtggacacgg gtgggcctgt ccgattcaga tgtagatgag ggggattacc
     9721 tgctcgattg gcgcagcggc ggcgagggcc tgcgatacgc gcaccatttt tcagcggagg
     9781 aactgcatgg gttggctggg caggctgggt ttaaggtgga atcggaattt ttatccgatg
     9841 gtgaaaatgg caggctgggg ctgtaccaaa tctgggcccc agactgaatc atctgctggt
     9901 tgccacgcgg ttgcgcccct tgtttttggc aatgtacatc gcctggtctg cgcgtttgat
     9961 gagaatatca agggacgtgg tgttttcatc cttgcaggcc acccccaaac tcgcagtaac
    10021 tgacagggtg aaattttcac cgatcttgac cggagaaccc gcgatcatgg cgcgcaggcg
    10081 ttctgccacc atcatcccca cttcaaggct ggtttcgggc agcaaaatga cgatctcttc
    10141 gccgccgtaa cggccaacga tatcgatctc acgcacacat tttttacaga aggaagccag
    10201 cgtcacaagt accctgtcgc ctgcatcgtg gccgtgggta tcgttgatct ttttgaaatg
    10261 gtcgatgtcg atcatgatgc aggaaaattc cttcctgcca aaaatcacat cttcaaattt
    10321 actttcaccc aaaagatata aaccgcggcg gttgtaaatg gtggtgagcg gatcaattat
    10381 ggcctgatcc tgggtctctt tgaagatgcg ggcattttcc agcgtgatcg aaacttgatt
    10441 ggcaaaggtt acagcgagtt caatatcatt actgttgaag tgattctcct cggcactgtc
    10501 aatggaaagc aggccgatga ccctgtcacg cacaaccagc ggcaccccca accaggagcg
    10561 gatgtgatca tgcggcgcct ggctgaaagc cttgtgcgct tttcgcacat cggcaaggat
    10621 atatggtttt ttggtttcga tcaccacact attcgggtta tcgccgggaa tctcaaacct
    10681 catccccttg atgttttcca catttgccca gccatgcccg cccacgatct ccaattcgtt
    10741 cccatccagt aattgaatgg atgcgctatc gtacggtacg acccgattca attgttcgag
    10801 gatatggttg accgcctcat ccatttcaag tgtgccagaa acaacggcac tggctttgcg
    10861 cagggtttca gaagttaccg cacggcgctg ggcgtattcc atggctttga atttctcgaa
    10921 cgccagcgcg atcaaattgg aagcctgggt acaaatctcg atctcttccg cttgaaaccg
    10981 atgatacaag ttaaaggtca ggatgatggc gccaagtcta ttcttcccgg ccaccaatgg
    11041 tatggccagc aaagattgcg cagggcattg ttctgcgatc tcacggtcaa aatatggaga
    11101 gttcgccaca tcctccacga tcagggtcgt tttgtagttc aaggccagtt cggtcagtgt
    11161 gaattcgccg aatacaggtt gaagcggaat gaaggtatta cgggaagcaa taaaagaagc
    11221 cagcggagaa gtttgtgaag cggcctcatc ccataccgta atgtaacaat catctgcaga
    11281 gatcaacccc gtcagccgcc ccaccaggtc ttcagccatg gcctttaagt tatcagcctg
    11341 caatgcttcc tgtacgattt gatgcagcaa ggtcagcgta ttctgcctgc gaatcaattt
    11401 ttgctcggca cgggtacgcg actcggcaga ccccaaaatg ctggcaatgg tgtgcagggt
    11461 ttttaattcc atgcccgtcc aatgcctgcg ggtgcggcat tcatcgaaca caacaaatcc
    11521 ccaccactcg ttctccacaa aaatgggcat ggccgccaca gacaggctgc ctaataattc
    11581 gaaaaccgca tgctcagatt ctggaaaatc ctccaccaac ccttgaatcg acaaatgctg
    11641 tgaaagttgt ttttcccaac gctgcaaccc agcctgttga agtgggatat gccgcaaggc
    11701 agggttgttg atctgcgata caacctcggg ggaaacccat tcatagcaca tgctggaata
    11761 cagggtgttc ttctcgtcct tgtaattcat tgccacataa acgcggctga cattcaccgc
    11821 ctgccccacc ttttccagca cagccaaaac attggcaccc caataacctt ccttcaaaaa
    11881 ttgttccgcc gacatgctca ccgccgacat gatcgcctca cggcggctga gcgcattttc
    11941 catttcctta cgctcggtga tgtcgtgcag tgtgatgatg caatcaggtg aaaatgttct
    12001 ctgctgctga tccttgtgga tgatgggaga aacggtcaca tcaaaaacgg tggtctgcct
    12061 gccctcgccg atcacaacct cagcccgggc atcttccgcc gtcaaatgcg gacgcaggat
    12121 gccccaatac aacgcagaga catgcgcaag cggctgccca atggcagtgt cttcgccgca
    12181 tcccaacaac ttctctgcaa tcggattcaa atacaaaaca cgctgttttg aattcaagac
    12241 gatcacaccg tctttcatgt tatttaaaac gcttaagtgc tcaagcggca gggtatcttg
    12301 caaacggtac cgtacaatcg cccatgaaag cccaagcccg gttggcagga acaacaacgg
    12361 cgttatatca aaattgggta tcggattcaa gccaaacaca aaaagcaggc tgccgatcaa
    12421 cggcaggaaa attcccaaaa tcaccaaccc aatctgtacc cgatccgatc ttggacgctg
    12481 ggctaattcc ctgaccagca gaatactggc aaataccgtc agtaaattcg caaacacaat
    12541 atgcacccaa aagaacaacc caaagcgaac atccaacaag tgcaggttac gaatttccac
    12601 caccttcgcc atggaccaga caagttggtg aaactcattg gtccatacaa gaaccagcat
    12661 catgaatggg aatatccaca acaacaggcg cagacgcggg gtgagtacat gactcttgcc
    12721 ggtaaactcc aaagcaaaga acaataacaa taccggaatg gaaacaatcc cgatgtattc
    12781 gacttttgca aaaaacaatt taaaagaaag gtacgggaag aaaagctcca aaccataccc
    12841 aaatgaccag atggacatac ccagcatgac ccaggcaaac ggggtcaccg ccactgcagg
    12901 gcgtttacgc catgcatgca aacccagcca gacatatgga attgcggcca gaatatatat
    12961 taatgagaca tagacttcgg tgcgattctg catacttact aaaaaacaca tcactaaagg
    13021 ctgtgatacg ccctttcgcc acttttaaaa accccgcgca ccttgatatg tcacagtgcg
    13081 cggagaagag ttgtctataa gattacctgc tggggattaa ccacatattg ccaccagtta
    13141 ttgtggatcc cattttttct ccctgccact cgtggaaaat gattgaacca ccatttatga
    13201 tgggctcgaa tatcgccgcc gccccactct gagggcccca ctgtgcgcac gtcacctttg
    13261 aaattgggga aattcaaaag ccagtcataa cattcgcttg gcaccggggt ggggttgttc
    13321 caatcgtaat ccctttggct gttgggcgca aaatggatgt ttccacaagc agccttgccc
    13381 ggcgtaacct tgtcatattg aataaaacgc ttccacaaat tggctggacc ggtcaacttc
    13441 gaaaaggtct tttccatgat cgattcagca cggtgaccat aagattcgtg catttcgccg
    13501 atgtatcttt cataagaaaa gcccatcacc acaaagcggc gtttgctagc ctctgtattt
    13561 ttcaacgtcg gggcgttgca ccaaaatgca ccggggccgc ccatcgtcga ctcataaaaa
    13621 cctgcatgtg gaaagccgaa ggtccacacc tcatcaatct caccacgggc cacccgctgc
    13681 aacacattgt gattggtaag aatggcgttg taattcactt cctgcggcac atgtggagag
    13741 gtaacaccgt gcagcacatc caggtaggtt tgcggagtat atcgaaaccc atcggtcttg
    13801 gcagggaact catccacatc cacccgttga acgatctgat accgtgccat ccccccgctg
    13861 acctggagca gatcggccat aaaccctgta atgaggtcgg cagggcgata ccatttttgc
    13921 tgctgcgaaa gcgtcacccc tgtggattta tccatcacag gatcgaacac gatcaccagc
    13981 accttactct gggtgatctg cgcgggttcc gtggcgctat caggtggata cttgggttgg
    14041 gaagatttaa aaagtttgga gaatgcgctg aaaaaatccg tcataatttc tcctgtatag
    14101 ggttgaagat cggcagcaaa gttttacggc atgtcggttg cgaaatagat ctgcccgccc
    14161 acagccagat aaagcacatg attgggacca accgcaatcg cccccacagg cccggaggga
    14221 atcggattca aagttcctgt tgtaggccgc aattgactct gaagctcaag tgagcgcggc
    14281 gtaaaacgca tgacaccctg cgtatcggca tccagcaaca ggatggattg attcggcaaa
    14341 gccgtcagca ggacctgtgt aaagtgagcg gctccaaaca aatctgtatc ctgatatgct
    14401 gcgatcgggt tgatcagcgg cgacggatcc tggcagcgcg tgggcaccga ctcgatgcgg
    14461 ctgtacgaac aggtggaaag atgcccatcg gcatgtaaca tgtacaagtc atcacctgaa
    14521 acgacaaggt caattacatc ctgtttttca ggggtctgcc cgccaaagaa gaaatatgga
    14581 cgatcaatga aggtgccatc tctgcctgtg taaacccaca cagcacgcgc cgaagaatcg
    14641 agcacgtaca aattgccgct atccaaaatg aaggcggtca cacggcccca attcgtatct
    14701 ggcggcggca atggaatggc ctgtgccacc tgtcctggcg cgcaatacaa aagattccca
    14761 gccgcatcca caccgagcaa ggttgcattg atcgagttaa gcgtgggcat ggtcaaaata
    14821 tccaccagcg gaccgaccgt ataactgcca tacacaccag gcttgcaatt gaaagcggta
    14881 tccaattcaa agccgcggcg gttactggct ggcacggcac ggagcgcctc tccattggcc
    14941 gcattcaaca aaaacaaatc tgtttcgctg gcagccatgc ggctgacctc gatgccgagg
    15001 ttaatgctga aagcagggtt gaactgcatc cgcgtgatgc ccagcaattt atcgagattg
    15061 acttcagcct gctcacgcaa ggtgatggta tcggatgtgg ggcgataaat atcttcggcc
    15121 ttatccacgt acaacaaaac attcttccag gattcgcgct gttcgatggg attcgtcagc
    15181 atggcggcct gcgtgctcat ttcacgcgcc tgcccgagga aaatctcata ttgcggcgta
    15241 cgcccatatt ggacatacac cacgaaaagc atcgccacca ccatcaatgg cacgcccacc
    15301 gcaatggcaa acatggaaaa gttggaaggc gcaaaactat cacccgcctc tgaattcggc
    15361 agcaagcgtg gaaggaagtt ccgtaacttc tcgccgatgg tctgattgat gcggcgggtg
    15421 gactggatgc ccgttgccag aacttttgct gtctggcggg tcatctcaga gggttcgcgg
    15481 ggcgtttcag gctcggcggc ctttacaggc gcaatgacct tttcctcaac acttttttct
    15541 tcctctgtct caatgggcaa agcctcttcc accatggcag ggggaaggtt tacctcagca
    15601 ctttgctgtg acggctgaat tctcggaatg gaggcgggga aatcacgcgg gacggtattc
    15661 cgcggcaggc tggccaagat cttattggca ggttctgttt cggttctctg cggcggaatg
    15721 gcatatgcgg aaggttgaac aacatgcgct gcgttttcgg gcttctcttc gtaattcaga
    15781 tcttcagttg gggcttccac aggttgaggt gcagaaactg tttcttcggc ctgtggcagg
    15841 ctcggggtag gcagaggcgc agccgtctct tccttttttc gttcgagtgt cccgtctaaa
    15901 agttttacat tccctgaacc attcgtggcc tgcatcaata cggcgttcac atccacactg
    15961 gtaagggtgg tcagccttcg acgcatggca tccagggaag aaggggcggg atcattgagc
    16021 gtgctatccc aggcatcggg cgcacgtcct acaaacaaca ggcggtcacc tgcattgagg
    16081 ttaagttggg catagtgaat attgatggtc tggctggcac ccaacccttt gccagaaaca
    16141 gcaggctcat gaatatggcg cgtatcttcg tgaccgaacc agtatgcatg catggggccg
    16201 ctcatcgaaa gcgtgcattg cgtatcgcgc aatgcggcca gggtcagcca ggcaatggcg
    16261 tattgtccgc gcgcgctgga tgacatgttg cgttcaagca gggtcttgtt cacatgctca
    16321 gaagcagctt tcagtgaact ggtaagcgac cccgcagatt gatagtaagc cgcagcggca
    16381 tcttgcgcca ccttcatata ttcgctggtc gagaagacag cattccccgt caaaagcaga
    16441 taaacgatca gacggtcctt ttcacggccc cgcgccgcag tacgcggcgg gttgagcgca
    16501 atcaaacctg gcaggctggt aatttcctgt ccattcaccc gatagatggg agcaagagtc
    16561 aaatcaagtg gcatgtaaga atagttccta atccgctatc attatactgg taaaatttgc
    16621 gcccatgaaa tacacactcc ataacgattt ttcagagatc aattcacagg aatggaacac
    16681 attgttgtcg gtttcgataa ccgacactcc atttttgcgg tacgaatatc agcgttcatg
    16741 gtgggagcac cgcggcggcg gcgagtggaa aaatgccgtc ctggtgctgg tcaccgcacg
    16801 cgaaaatgaa aagctcattg gcattgcccc tcttttcatc gccgaatacg acggacgatc
    16861 tgcgcttctc ttaaatggaa gcattgaaat ctccgattat cttgacctga tcgtcgggca
    16921 ggatgaccat acccgcttcg tttcaggtct gctggatttt ctaacggctc aaagcggacc
    16981 cgccaactgg agcggcctcg attggtataa cctccccgac tcatccccca cccttgccgc
    17041 cctcaaagca gaatctgcaa ctcgcggctg ggcacatcac gaggaaatgt accgccccac
    17101 accccgcatt gccctgaacg gcagtttcga agaatatctt tcacgtgtgg aaaaaaagca
    17161 gcgccatgaa atccgccgta aagtgcgccg cgccgaagaa agtgaacgtg gggttcgctg
    17221 gttcatttca gatatggccg atgtggaagc tgaaatcgat tcattcatca gcctgatgga
    17281 gcaggaccaa aacaaggcaa gtttcctgca tccgccgatg cgcgcccaaa tgcgcgatgt
    17341 catccgcacc gcccaccaaa atggctggct ctggctggcc ttcctcgagg cggatggtca
    17401 acgtattgcg gcggcgctca actttgatta caacaataaa ttatgggggt acaacgcagg
    17461 cgtcaaccgc gcctacatgg acctttcccc cggctgggtc ctgctggctt atattttgga
    17521 gtggtgctgt caaaacggac gcagtgaatt cgatttcatg cgcggtgacg aagaatacaa
    17581 ataccgcttc ggcgccgtga accaatacgt gatgcgggca aaactcacac gggcttaa
//
LOCUS       DAOQJC010000086        17472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1533_length_17472_cov_10.323190, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000086 DAOQJC010000000
VERSION     DAOQJC010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 17472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17472
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1533_length_17472_cov_10.323190"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="PLE39_13010"
     CDS             <1..1197
                     /locus_tag="PLE39_13010"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNE69296.1"
                     /translation="IGPSADSIRAMGDKAESKIAMKKAGVPTVPGFEGLTSEEEFKKA
                     AQEIGYPVLIKASAGGGGKGMRVVNFENELSEAIESARREALNSFGDERLLIEKYLAD
                     AHHIEFQVFGDKHGNLVHLFERECSVQRRHQKIIEETPSPLLTPEIRAKMGEAAVAAA
                     KAVNYYNAGTIEFIFDPQLATLNLQPFYFLEMNTRLQVEHPITELTAGIDLVQWQIRI
                     AAGEKFPFTQGDFHQHGHAIECRVYAEDPSNGFLPSTGKLLQLIEPRGPGIRVDSGFK
                     TGSEVTHFYDPLLAKLIVHAENRNAAIQRMQTALREFVVHGVVTNMDFMQAVLQHDDF
                     AQGKVSTRWVERNFGEWHPVEAEKEALIAAALADVLFVGGKTQSAVSNETDPFNPWKQ
                     TGNYRN"
     gene            1200..1718
                     /locus_tag="PLE39_13015"
     CDS             1200..1718
                     /locus_tag="PLE39_13015"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNE69297.1"
                     /translation="MKTTFDTQTLEITPSGDHYLAVMDGRKILVEVIRVDAAQGRMDL
                     RFTELSESDSTRLSVSKSVYVSSDMAKRWVTVDGHTTMLTKTSGAKQGVRHDHAGGLI
                     APMPGQVRSVAVGVGDVVKKGQTLLTMEAMKMEIRIQALKDGVVSALPVQVGQTVERE
                     QILIEIVDRVEH"
     gene            1761..2258
                     /locus_tag="PLE39_13020"
     CDS             1761..2258
                     /locus_tag="PLE39_13020"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNE69298.1"
                     /translation="MAGKYFEDLDVGMHFKHSAGRTVTEMDNVLFSSLTMNTQPLHVN
                     EDFAANTEFGQRIVNGIFTLGLVVGLTVAELSEGTIIANLGYDKVVHPNPTFHNDTIY
                     AETEVLEKRESKSRSNAGIVKFRHTGKKSDGTIVVQFERTALFLKKGQRLDFLPRSES
                     QSYDL"
     gene            2312..3178
                     /locus_tag="PLE39_13025"
     CDS             2312..3178
                     /locus_tag="PLE39_13025"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HNE69299.1"
                     /translation="MHSRRALLYMPGDNWKMITKSITLGVDSICMDMEDGTAINKKAE
                     ARETIAKALRELDFGSSEKLARINSVGSGWEQDDIDAVLPFHPDGIVIPKVESFEQVE
                     WASRIIETAELKYGWPVNSVRVLIGVETAKGILNLKEIAAHPRLDAIIFGGEDFAASI
                     GAVRTKDAVELLYARQAVIVACSAFDLQPIDIVTIDYKDIEALRVESEFGARLGFVGK
                     QIIHPAQVEPVQRAFTPSDEAIAYAKRIVETFEASQKEGKGAYSLDGKMIDMPLLRNA
                     KKVLDRALDRGL"
     gene            3237..3773
                     /locus_tag="PLE39_13030"
     CDS             3237..3773
                     /locus_tag="PLE39_13030"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNE69300.1"
                     /translation="MKWSTSPHRPPHLYVDGAWYFITSSTVGSAKILSTDAHLNLWVE
                     TFKELILQFKIKLIAWVILPNHYHILFMPEKADELGSFMKRLNGNTSRKLNLFDDQAG
                     RSVWYSYWDTCIRNERDFWTRFNYIHYNPVKHGYVEYPGEWRFSSYSFYLRNDESPWL
                     AGYEKDFPIYDLFDDDRF"
     gene            3845..4690
                     /locus_tag="PLE39_13035"
     CDS             3845..4690
                     /locus_tag="PLE39_13035"
                     /inference="COORDINATES: protein motif:HMM:NF018506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HNE69301.1"
                     /translation="MLSIFLGLLAALGWGAGDFTGGIASRKTGAYRTVFYGELIGVFA
                     LFLVIAIISEPIPDLRSWLFAMLAGALGTLGLILLYHAMAIGVMSIAAPVSALLAAAL
                     PVVVGIFREGLPAWTTFIGFGFALFAVWMISQGEGGVTDIFSHLADLKLPLIAGIGFG
                     SYFVFMHEATSTGSTIWPMVASRSGGMILITIYMLVVRVEWKVEDRSAWPIIVLNGIL
                     DISGNVFFILAGQMGRLDVAAVLSSLFPGTTVMLAWIFLKERLSPTQWIGIASALTAI
                     VLMTI"
     gene            4699..5355
                     /locus_tag="PLE39_13040"
     CDS             4699..5355
                     /locus_tag="PLE39_13040"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNE69302.1"
                     /translation="MNPYLLFAFIFALLEALALWKGWIRLEYVAKPAVMIVLLLWLWT
                     SVGISGALLWFGVGILFSLLGDVLLMISLDRMFLLGLVAFLLAHIAYVIGFNIPLPVF
                     SAWSLVFAFMVGLGGTRIIRRILEAIGNNRMRKPVIVYSLVISLMLLSAMMKLTDVTW
                     DAGAAALVSLGAFLFYLSDIVLAWNKFVAPIQNGRIYNIGLYHLGQIALIAGVIAQFG
                     "
     gene            5407..5844
                     /locus_tag="PLE39_13045"
     CDS             5407..5844
                     /locus_tag="PLE39_13045"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE69303.1"
                     /translation="MQSILVIIIIGLVGGMAVGLQAPMSSMISQRLGVLESIFIIHLG
                     GALVVLLPLLYFGGGKLGHWRTVPWYALCAGAFGLVVIFSMSYMIPRIGIATALIILL
                     AGQILIGSILDHFGLLGAAVRPLDFTRVLGLGVVLAGVWLAMK"
     gene            5927..6691
                     /locus_tag="PLE39_13050"
     CDS             5927..6691
                     /locus_tag="PLE39_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69304.1"
                     /translation="MYNYPLKFEFPMISLGRQMDVKNGDGGFIMRVSDPYISFKDEMT
                     VINGKGAAVMKAKGDSSFRFLFQFASTSTSWNIVDGSDRTIAWVTNYWARMEEFKDVY
                     VDTPSGVGTDGRADIGGVLGNIATNVLNNRVASTLPSRLVYKITDSEYGNVLGWVVPS
                     RGTGWFDFLPYSTRIQILNLPFAARAYAPSYEFKLGNLYGPTVLKLQKQRDLLVDKYT
                     LEKIGDLSEANERWALPSIALAAMFERQRVKEMADW"
     gene            complement(6799..8679)
                     /locus_tag="PLE39_13055"
     CDS             complement(6799..8679)
                     /locus_tag="PLE39_13055"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE69305.1"
                     /translation="MAGSRKLQYADRVALQQADQAIRKDVLRALVEIITNSNDSYSRL
                     EHAGLKVSGEIIIDIQRKHKNSIIRVTDRAEGMTDTRMDKVVGTYGEATSGLKEDKNV
                     RGMWGRGLKDSIFGLGYGYVHSFKGAYFYSCALLLKNGIPTFELLPPVRATVPLREKY
                     GIPEGNGTILEIIVSRDDVKVPQFDNFRNYLQRHFELRPIMSNPKRKIILRDVPSAEK
                     IRHEHVLSYKSPRGEKVLSEKIKIEGYPAAAKLEVFRSAIQLSTRGEEGDYADGGLLV
                     ISRATVISLTMLKFENDPYAGYFYGSIQCDHLHDLLKNDEPVLTATRDGINWSHPFAK
                     ALKLAVENKLEPLIEAERKHAVQDEQTHLDKQFRQKIDHALHELNTIALNELSDRRDD
                     GDQKRIDLPPSGIGFFPERVHVQTGQTISLTLFVKLSEKVRPGATVTVISNSPEVIVL
                     NQQAVLQPHKTDDSVGRAHIRVEGRQVGGEGLVTAYIGKQRAQALVQVRSKKETLTIA
                     PSRSSNALFTDIHFDDRTDPRQRVYFDRVNSSIVIATAAPSVKLYLDKQNRLDTTVQG
                     QVLLAELITEAVCREIARSGVERGRFLAPDGAEADAINNHFIRLQNQYSHLIHKYIVT
                     LE"
     gene            complement(8706..>9152)
                     /locus_tag="PLE39_13060"
     CDS             complement(8706..>9152)
                     /locus_tag="PLE39_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69306.1"
                     /translation="KKKEVAVVTPVVLKPAKKKEEKAVVPVVSEPVKKKEEKAVVPVV
                     VSQAVSREEKARAAKFVRAMEVSDAYRLYKKNGKESKISEFDFRSLLLCTMESSSETL
                     ARNVELYKGYASIHNRQDLLTFLGYCETSFETLLKPGPVKKAAKKK"
     assembly_gap    9153..9252
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9253..9745)
                     /locus_tag="PLE39_13065"
     CDS             complement(<9253..9745)
                     /locus_tag="PLE39_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69307.1"
                     /translation="MTAKTSGARKLKRRSLPKFDPSVYSAISSVNLVAYAIHFLLERG
                     IEIHMEDVVFGCFLLFPHKFALKKYPRWPDSAVVGRRVGDCRSKGYIAANTEFGFKLT
                     VKGTLAAERAAKALGIFKVQKPGLPQKPAEKVIAQQAVQLTLLETKAVVAPVVVSEPV
                     KKKE"
     gene            9877..11604
                     /gene="polX"
                     /locus_tag="PLE39_13070"
     CDS             9877..11604
                     /gene="polX"
                     /locus_tag="PLE39_13070"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HNE69308.1"
                     /translation="MNNRQLADTFTLIANLCEIKGEVIYVILAYRKASENLMTLGREA
                     SEYWKEGKLREIPGVGKAIAEKIDELLTTGKLQFLENLKEEVPASLADWLQVPGLGPK
                     KIALIWKTLNITALSELETAAKNGQLRGLPGMGAKSESAILEGIASLARRSGRIPLGR
                     AYPLAQEIIRTLKRVDGVVAAESAGSLRRMRSTVGDLDILVASTNSAAVMEAFTNLPG
                     VSRVLGKGETKSSIEFTDGVRAQVWVHAPEKFGTALQYATGSKDHNVQLRQIALAKGL
                     SLSEHSLTKTNGKGEIFCATEEEVYERLGLPWIPPELREDHGEVVAAKAGSLPKLIQV
                     KDIKADLQMHSNWSDGKLTMLEMAQAAIAHGLKVIAFTDHSASLGVTGGLKMEDHKKQ
                     AAEIKKVQKQLGDKILILHASEVEIKADGTLDYPDEFLASLDLVLASMHTSLRQPREK
                     VTERMIAAIRNPHVDIIGHPTGRLIPDREGADLDMEAVLNAAAETGVALEINAHPSRL
                     DLDDMYARRAKELGIPISINTDSHSAEDFDNLFYGVATARRAWLTKEDVINTWSTKKL
                     LNWLEKRGK"
     gene            11601..11873
                     /locus_tag="PLE39_13075"
     CDS             11601..11873
                     /locus_tag="PLE39_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69309.1"
                     /translation="MKALLKPSLTKILLALGLFIVASFLWKYTFGLVIMDVNYFGWPL
                     HYLTTWGPCRAGENCSEFNGMYLVFDFLFWYMIGAWIVNRFSKKTT"
     gene            11887..>12860
                     /locus_tag="PLE39_13080"
     CDS             11887..>12860
                     /locus_tag="PLE39_13080"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNE69310.1"
                     /translation="MAESLIPANEVRFFSGSSNPQLAGKIALHLGVPLEDTHISRFSN
                     DNLYIQLGASVRYRRVYIVQSLSKPVNDHLMELMMMIDIARSAAASEVHAIIPYFSFG
                     RSDKKDAPRISITARLVADLLKTAGATHVMSIMFHSPQVHGFFGIPTDPLSSSKVFKE
                     YLSKSDLSGSILVAPDMGQAKTAARFARTLNLPIAAGNKERVSDTKVIINGLVGDQVR
                     GHERALIYDDEIATGGSILELSRVLISEGLKDIRVICTHGVFSNNGLERLAEVPEITE
                     IITTDTVHIEPERVHPKLTVLSVAPVFANAIRHNINRESLSDLFVYGE"
     assembly_gap    12861..12960
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13039..13533
                     /locus_tag="PLE39_13085"
     CDS             13039..13533
                     /locus_tag="PLE39_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNE69311.1"
                     /translation="MINKTMQDAMNDQINKELFSSYLYLSMAAYFEDKNLPGFAHWMR
                     IQEAEEREHAMKFYDFILERGGRVMLKAIEAPKTDWKSALEVAEEVAAHEAKVTASIH
                     ALYELALKEKDYPAQVMLQWFISEQVEEEKNAAEIVANLKLIEERGTAVLMLDHRLGK
                     RGGE"
     gene            13592..14032
                     /locus_tag="PLE39_13090"
     CDS             13592..14032
                     /locus_tag="PLE39_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69312.1"
                     /translation="MQITIRRATHADKGEWFQMRKGIWPDVPDHYMSFDMDDLLASEN
                     YLVLFACDGETPIGLTEARLRDHAEGCSTSPIGYIESWYVREDYRGRGVTGIMNQAAE
                     EWARSKGCTEMASDTWLDNEASIRAHLKLGYFEVERLVHFVKKL"
     gene            14270..16180
                     /locus_tag="PLE39_13095"
     CDS             14270..16180
                     /locus_tag="PLE39_13095"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE69313.1"
                     /translation="MSSIRLRVVIPYLLLTLIVSGIGVYVVTNLVSNTLSERLANQLV
                     QSGRVVLDGFVKQEQSQIEDARFIAFTSGVADALVSGTADGLLVEQATTQMTGRDIDT
                     LVFISSQGNEILHLWKNDLNVVQRANENSGMAGSPLVTPFLASRNTRDNPRHFLGTNS
                     LNDRTYFYASIPASLNGEFAGVVVIGIDIEKLLPNLKSSALADVVVYGADGSVIATTL
                     DVSDPLSLEALSITNDNYQRISALTDLVTGSNISIDRRSYSVARGSLQLGNDKLGVFA
                     VALPLDFVNQAVSENQTTYTLLFIGVFFFVIVIGLWVSRSIVNPLYQLVETSQAISAG
                     DLEKRTGIKSRDEIGILASAFDEMTANLQERNAQLERANQILKNIDKTKSNFIQISAH
                     ELRTPLTLIMGYSQMLEQDARNDPELKSLAQGILDGSERMTSIVESMLDVSRIDSNSL
                     FLRKTGLRIDLIVRKVYKNFEKALVERDLQFDMNNLRDLPLIPADPDMLQKVFYHVIM
                     NAIKFTPDGGSITISGRYVPANEPSVEISIRDTGIGINPEMHSLIFEKFNQTGDVLLH
                     SSGKTKFKGGGPGLGLAIARGIVEAHGGRIWVESPGYNEETNPGSTFHISLPAPEDQQ
                     EEVRREPAISKQ"
     gene            complement(16385..16972)
                     /locus_tag="PLE39_13100"
     CDS             complement(16385..16972)
                     /locus_tag="PLE39_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69314.1"
                     /translation="MPTTNSGGTVVGVPEKTGVLVGVAVKVEVAVKVAVGDGVFVAVA
                     VEVAVFVAVAVNVAVFVAVGMTVNVAVAEAVGVDVGVTVAVEVEVAVLVGVAVLVAVN
                     KDVAVRVAVMVGVRDTEVEVRIGVRVAVRVDVTATTVERLVLVAVGGEVRVAVGAERW
                     VEVAVAGGRWVKVAEPFGLIGPEEEPEAVPVPGVR"
     gene            17025..17438
                     /locus_tag="PLE39_13105"
     CDS             17025..17438
                     /locus_tag="PLE39_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69315.1"
                     /translation="MTSNPIEVKNPADGFYDFIFYELFVAPDRINMDRIILSISMDGT
                     NYYEVFNWGDNIPDNNTNIYTYTPENDNLPIFTTVLYGVPLLQTGIVVDVDNVASNPP
                     PGLYIYLAIEVPAGPINDGAGIDAIQVQEVPIPFP"
BASE COUNT         4040 a         4194 c         4586 g         4452 t
ORIGIN      
        1 atcggccctt cagcagattc gatccgcgcc atgggcgata aagccgaatc gaagatcgcg
       61 atgaaaaagg cgggcgtgcc aaccgtccct ggctttgaag ggctgacatc agaagaagag
      121 ttcaaaaaag ccgcacagga aattggctac cctgtattga tcaaagcctc tgcgggcggc
      181 ggcggcaaag gtatgcgtgt ggtcaatttt gaaaatgaat tgagcgaagc catcgaatcc
      241 gcgcggcgcg aagccttgaa ttccttcggt gatgaaaggc tgctgatcga aaaatatctc
      301 gcagacgcgc atcacattga gtttcaagtc tttggcgata aacatggaaa ccttgttcat
      361 cttttcgagc gcgaatgttc cgtgcaacgc agacatcaaa agatcatcga agagacaccc
      421 tcaccgttgc tcacgcctga gatccgcgca aagatgggtg aagccgcagt tgcggcggcc
      481 aaagccgtta attactacaa cgctggcacc atcgaattca tcttcgaccc ccaacttgca
      541 actttaaacc ttcaaccttt ctacttcctc gaaatgaaca caagacttca agtcgagcat
      601 cccatcaccg aactcaccgc aggcattgac cttgtccagt ggcagattcg tattgcagca
      661 ggagagaaat tccccttcac gcaaggtgac tttcaccagc acggacatgc catcgagtgc
      721 cgtgtttacg ccgaagaccc atccaatgga tttttgccca gtacaggaaa acttttgcaa
      781 ttgatcgaac cgcgcggtcc gggcatccgc gtggattcgg gcttcaaaac gggcagcgag
      841 gtgacgcatt tctacgatcc gctgttggcg aaattgatcg tccatgctga gaaccgcaat
      901 gcggccattc aaagaatgca aactgcctta cgcgagtttg ttgttcatgg cgtagtgacg
      961 aatatggatt tcatgcaggc agtcttgcag catgatgact ttgcgcaggg caaagtgtcc
     1021 acgcgttggg tggagcggaa ttttggtgag tggcatcctg tcgaagctga aaaagaagcg
     1081 ctgatcgctg cggcgcttgc ggatgtgctt tttgtaggcg gcaagaccca atccgctgtt
     1141 tcaaatgaaa ccgatccctt caacccgtgg aagcagacag ggaattatcg gaattagcca
     1201 tgaaaacaac cttcgacaca caaactcttg agatcactcc gtcgggagat cattaccttg
     1261 ccgtgatgga tggcagaaaa atcctggtcg aggtcattcg tgtggatgcg gcgcaggggc
     1321 gcatggattt acgcttcacc gaactttcag agtccgattc cacccgttta tccgtttcaa
     1381 agtccgttta cgtctcttcc gatatggcga agcgctgggt gacggtggac ggtcacacaa
     1441 ccatgctcac gaaaacctcg ggcgccaaac aaggcgtcag acatgatcat gccgggggat
     1501 tgatcgcccc gatgccgggt caagtgcgaa gcgtggctgt gggtgtggga gatgtcgtta
     1561 agaagggtca aacccttttg acgatggaag ccatgaagat ggaaatccgc attcaggctt
     1621 tgaaagacgg cgtggtttcg gcgcttcccg ttcaggttgg tcaaacagtg gagagggagc
     1681 agattctgat tgagatcgtt gacagggtgg aacattaaac gttcaacctt taaacctgca
     1741 aacgttccaa cggaggttgt atggctggaa aatattttga agacctggat gtggggatgc
     1801 atttcaaaca ttcggcgggg cgtacggtga ctgaaatgga taatgtactg ttttcttcgc
     1861 tgacgatgaa tacgcagcca ttgcatgtga acgaggattt tgccgcgaac acggaatttg
     1921 gtcagcgtat tgtgaatggg attttcacgc ttgggctggt ggtgggcctg accgtggctg
     1981 aattgagcga gggaacgatc attgccaatt tggggtatga caaagtggtg catcccaacc
     2041 cgacttttca caacgatacg atctacgctg agaccgaagt gctggaaaaa cgtgagtcga
     2101 aatcgcgctc aaatgcgggg atcgtgaagt tcaggcacac aggaaaaaaa tcggatggta
     2161 cgattgtggt ccaatttgaa cgaacggctt tgtttttgaa gaaaggtcaa cgcctcgact
     2221 ttttgccaag atccgaaagc caatcttacg acctataatc tggaacgtta aaacgttaga
     2281 acgttccagc gttgaaacag gagacctcct catgcactca cgacgcgcac tactttacat
     2341 gcctggtgat aactggaaga tgatcacaaa atccatcacg ctgggcgtgg attcgatttg
     2401 catggatatg gaagatggta cggcgatcaa taaaaaagct gaagcgcgcg aaaccatcgc
     2461 gaaggctttg cgtgaattgg attttggctc cagcgaaaaa ctggcgcgga tcaattcggt
     2521 cggttcaggt tgggagcagg atgatattga tgcggttctg ccgtttcacc ccgatgggat
     2581 cgtgattcca aaggtggaat cttttgaaca ggtggagtgg gcgagccgca ttatcgaaac
     2641 tgctgaattg aaatatggct ggcctgtgaa ttctgtccgc gttttgatcg gggtggagac
     2701 ggcaaagggc attttgaatt tgaaggagat tgccgcacat ccgcgtttgg atgcgatcat
     2761 ctttggaggc gaggattttg ccgcctccat tggcgcggtc cgcacaaagg atgcagttga
     2821 gttgctgtat gcgcggcagg cggtgatcgt ggcatgttcg gcgtttgacc ttcagcccat
     2881 tgatattgtc accattgatt acaaggatat cgaagccttg cgagttgaat ctgagtttgg
     2941 tgcgcggctt gggttcgtgg gcaaacagat catccaccct gcgcaggtgg agcctgtgca
     3001 aagggctttt acgccatcgg atgaagcgat tgcgtatgca aaacgaatcg ttgaaacttt
     3061 tgaggccagc cagaaagagg ggaagggtgc gtactcattg gatggcaaga tgatcgatat
     3121 gccgctgttg aggaatgcga agaaggtgtt ggatcgtgct ttggatcgcg gactttagtc
     3181 cgcgtttgat ctgttttgca gactaaagtc tgcgatccga gagaggaaaa aatccaatga
     3241 aatggtccac atccccgcat cgcccgccgc acctttatgt ggatggcgcc tggtatttca
     3301 ttacatcctc caccgttggt tcggcgaaaa tcctttccac agatgcccat ttgaacttgt
     3361 gggttgaaac gttcaaggaa ctcatactcc aatttaagat aaaactgatt gcatgggtga
     3421 tcctgccgaa tcattatcat atcctgttca tgccagagaa ggctgatgaa cttggttctt
     3481 ttatgaaacg cctcaacgga aatacttcgc gcaaattaaa tctctttgat gatcaggctg
     3541 gaagatcggt ttggtatagt tattgggata catgtattcg taatgagcgt gatttttgga
     3601 cgcggttcaa ctacattcat tacaatccag tcaaacatgg ttatgtggag tatcccggag
     3661 aatggcgttt ttccagttat tctttttacc tgcgtaacga cgaaagccca tggcttgcgg
     3721 gttacgaaaa agatttcccg atttatgatt tgttcgatga tgatagattt tgatgtgagc
     3781 gcggacttta gtccgctaat aataaatgcg gactgaagtc cgcacccctg aaggagagta
     3841 aacattgcta tctatttttc ttggtcttct cgccgcgctg gggtggggtg caggcgactt
     3901 tacgggcggt attgcctcgc gtaagacggg tgcctatcgt acagtgtttt atggcgaact
     3961 catcggcgtc tttgccttgt ttcttgtcat tgcaataatt tctgagccca tccccgatct
     4021 gcgctcatgg ctgtttgcca tgctggcggg cgcgctcggt acattgggtt tgatcctgct
     4081 gtaccatgca atggccatcg gcgttatgag cattgccgcg cctgtatccg ctttgctggc
     4141 ggcggcactc cctgttgtgg ttggtatttt ccgcgaaggc ctgcctgcat ggacaacctt
     4201 cattggattt ggcttcgccc tcttcgctgt gtggatgatc tcgcaagggg aaggcggcgt
     4261 gaccgacatc ttctcgcatc ttgccgactt gaaactgcct ctcattgcag gcattggatt
     4321 cggctcatac tttgtcttca tgcacgaagc caccagcaca ggctccacca tttggccgat
     4381 ggtcgcatcc cgttcgggtg ggatgatctt gatcaccatt tacatgctcg tcgtccgcgt
     4441 tgagtggaag gtggaggatc gatccgcgtg gccgatcatt gtcctcaatg gcatccttga
     4501 tatttctggc aatgtttttt tcatcctcgc aggccaaatg gggcgtttgg atgtggcagc
     4561 cgtgttgagt tcgcttttcc ctggaacaac ggtcatgctg gcgtggatct tcctcaagga
     4621 acggctttcc cctacccagt ggatcgggat cgcctccgca ttgaccgcga tcgttctgat
     4681 gacaatatag gacgtgaaat gaatccatac ctgctttttg cgtttatctt tgctttgctc
     4741 gaagcgctgg ctttgtggaa aggctggatt cgtttggagt atgttgccaa acctgctgtg
     4801 atgatcgttc tattgctctg gctgtggacc tctgttggaa tcagcggggc tttgctttgg
     4861 tttggggtgg ggattctttt ttcactcctt ggcgatgtgt tgttgatgat ctcgcttgac
     4921 cgcatgtttt tattggggct ggttgctttt ctgctggcgc atatcgctta tgtgatcgga
     4981 ttcaacattc ccttgcctgt cttttcggcg tggagtctgg tctttgcttt catggttggc
     5041 ctgggcggca cgcggatcat ccgccgaatc cttgaggcaa ttggaaacaa ccgcatgcgc
     5101 aagcctgtga tcgtctatag tctggtcatc tcactgatgc tgctttcagc catgatgaaa
     5161 ctgaccgatg tcacatggga tgcgggtgcg gcagccctgg tcagcttggg tgcatttttg
     5221 ttctacctct ccgatattgt cctcgcgtgg aataaatttg tggctcccat tcaaaatggg
     5281 cggatttaca acatcggttt gtatcatctc ggccagatcg ccttgatcgc cggggtgatc
     5341 gcgcaatttg gttagggcgt cttcaggtga gctaaaaggg attcatttca aacaaaggca
     5401 ggcaaaatgc aatcaattct tgtaattatc atcatcggtc ttgttggcgg aatggcggtt
     5461 ggtttgcagg ctcccatgtc cagcatgatc agtcagcgtt tgggggtact tgaaagtatt
     5521 ttcatcattc accttggcgg cgctctcgtg gttttgcttc cgctcttgta ttttggcggt
     5581 ggaaaactgg ggcattggcg gactgtcccc tggtatgcgc tgtgcgcggg ggcctttggc
     5641 ctggtggtaa ttttttcgat gagttacatg attccgcgca ttggtatcgc cactgcgttg
     5701 atcatcctcc tggcagggca gatcctcatc ggctccattc ttgaccattt cggtttgttg
     5761 ggggccgcag ttcgtccatt ggatttcacc cgtgtacttg ggctgggtgt ggttctggcg
     5821 ggggtgtggc tggcgatgaa atagacccca ttacttttga cccatatcac cccactcctg
     5881 tgatttgtat aatcgcggaa aattatttca caggagtttt cttttcatgt acaactatcc
     5941 cctcaaattc gaattcccga tgatcagcct tggccgtcaa atggacgtca aaaacggcga
     6001 tggcggtttt atcatgcgtg tttcggatcc ctacatttcg tttaaggatg aaatgaccgt
     6061 cattaatggc aagggggccg cggttatgaa ggcgaagggt gatagctctt ttcgcttttt
     6121 gttccaattt gccagcacct ccaccagttg gaatattgtg gatggttctg atagaacgat
     6181 cgcatgggtg accaattatt gggcgcgcat ggaagaattc aaggatgtgt acgtcgatac
     6241 gccttccggt gtcggcacag atggacgtgc ggatatcggc ggcgtgcttg gtaacattgc
     6301 cacgaatgtg cttaacaatc gggttgcttc aacccttccc agcaggctcg tttataagat
     6361 cacagacagc gagtatggaa atgtattggg ttgggtggtg ccttcacgcg gtacaggctg
     6421 gtttgacttc ctgccctatt cgacccgcat tcagatcctc aacctgcctt ttgcggcgcg
     6481 tgcctatgcc ccttcctatg aattcaagct tggaaacttg tatggcccca ctgtgctcaa
     6541 actgcaaaag cagcgggatc tgttggtgga caaatacact cttgaaaaga tcggtgactt
     6601 aagcgaagcc aatgagcgct gggcacttcc ctccattgca ctggctgcca tgttcgaacg
     6661 tcagcgtgtg aaagaaatgg ctgattggta aatcaaccaa ctacaactta aagcaaagcc
     6721 cgcccacgaa actttcgggg cgggcttttt ttattgaaac agaatcaatt tacagtttcg
     6781 tcagacgcgt tgtctttcct attccaaagt cacaatgtat ttatggatca ggtgtgaata
     6841 ctgattttgc aagcggatga agtgattgtt gatcgcatca gcttcggcgc cgtcgggtgc
     6901 aagaaatctg ccgcgctcca caccgctgcg ggcgatctcg cggcacacag cttcggtgat
     6961 caactctgcc aaaagcacct ggccttgcac tgtggtgtcg aggcggtttt gtttatcgag
     7021 gtagagcttt accgaaggcg cggcggtggc aatgacaatg cttgaattta cgcggtcaaa
     7081 atatacacgc tggcgcgggt cggtgcggtc gtcgaagtgg atgtcggtga acagggcatt
     7141 gctgcttctg gatggagcaa tggtcagcgt ttctttttta gagcggacct gtaccaaagc
     7201 ctgtgctctt tgttttccaa tgtaggcggt caccagccct tcgccgccca cctgcctgcc
     7261 ttccactcga atatgcgcgc gcccgacact gtcatcggtt ttgtggggtt gaaggacagc
     7321 ctgttgattg agcacgatga cttcagggct gttggagatc acagtgactg ttgcacctgg
     7381 gcggaccttt tcagataatt ttacgaacag ggtcaatgaa atggtctgcc ctgtttgcac
     7441 atggacacgc tcagggaaaa aaccgattcc tgacggcggc aggtcgattc gtttttggtc
     7501 gccgtcatcg cgcctgtcac tgagttcatt caaggcgatg gtgttcaatt cgtgcagggc
     7561 gtggtctatt ttttggcgga attgtttatc gagatgggtt tgctcatctt gaacggcgtg
     7621 tttgcgctcg gcttcaatga gcggttcgag tttgttttca acagccagtt tcaacgcctt
     7681 cgcaaatgga tgcgaccagt tgatgccgtc acgagtggcg gtgaggacgg gttcatcgtt
     7741 cttcaacagg tcgtgcagat gatcgcattg gatcgagccg tagaaataac ccgcatatgg
     7801 atcgttctcg aacttgagca tggtcaacga aatgaccgtg gctctgctga tgaccagcag
     7861 gccgccatcg gcataatcgc cttcctcgcc tcgggtggat aactggatcg cagaacggaa
     7921 cacttccagc tttgccgcgg cagggtaccc ttcgatcttg atcttctcac tgaggacctt
     7981 ctcgcctcgc ggtgacttgt agctgagtac gtgctcgtga cggatcttct ccgctgatgg
     8041 aacatccctg aggatgattt tgcgcttcgg gttgctcatg atcggacgaa gctcgaaatg
     8101 ccgctgtaga tagttgcgga agttgtcgaa ctgcggcact ttcacatcgt cacgcgagac
     8161 gatgatttca aggatggtgc cattgccttc ggggatccca tatttttcgc gcaggggaac
     8221 tgtggcacgt acgggcggca gcaattcaaa ggtgggaatt ccattcttga gcagcagcgc
     8281 acaggaataa aaatatgcgc ccttgaacga atggacatat ccatacccca gcccgaaaat
     8341 ggaatctttc aagccgcgcc cccacatgcc gcggacattt ttatcttcct tcaatccgct
     8401 ggtggcttcg ccgtaggtgc caacgacttt atccatgcgg gtgtcggtca tgccttcggc
     8461 gcggtctgtt acgcggatga tggagttctt gtgctttcgc tggatgtcaa tgatgatctc
     8521 gccgctgacc ttcagccctg cgtgttcaag ccgcgaatag ctgtcgttgg agttggtgat
     8581 gatctccacg agcgcgcgca aaacgtcctt gcgtatcgcc tgatcggctt gttgaagtgc
     8641 tacgcggtcg gcgtactgta atttacgact gcctgccatt aataaaaaac ctttctctct
     8701 tctctttact tctttttagc ggccttcttt acaggacctg gttttaacaa ggtttcaaaa
     8761 gaagtttcac aatatccaag gaatgtaagt agatcctggc ggttgtgaat gctggcatac
     8821 cctttgtaga gttccacgtt ccttgccagt gtttcgcttg aagattccat cgtgcataac
     8881 agcagacttc tgaaatcgaa ttcgctgatc tttgattctt tgccgttctt tttgtacagg
     8941 cgataggcat ctgaaacttc catcgcacgg acgaattttg cagcacgcgc cttctcctcg
     9001 cgtgagacag cttgcgaaac aacaacaggg acgactgcct tttcttcttt tttctttaca
     9061 ggctctgaaa caacaggaac aactgctttt tcttctttct tctttgcagg tttcagaaca
     9121 acaggagtaa caaccgcaac ttctttcttc ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nncttctttc ttcttcacag gttccgaaac aacaacagga gcaacgaccg
     9301 ccttcgtttc caacaatgta agttgaactg cttgctgtgc gatcactttc tcagcaggtt
     9361 tctgaggcag gcctggtttt tgtactttga aaattccaag cgctttggca gctcgctcgg
     9421 cagccagagt cccttttacg gtgagtttga atccgaattc tgtgtttgcg gcgatgtacc
     9481 ccttgctgcg gcaatcgccc acccgcctgc caaccacggc agaatcaggc cagcgcggat
     9541 attttttgag ggcaaattta tgggggaaca gcagaaaaca accaaaaacc acatcctcca
     9601 tatggatttc gatgccgcgt tcaagcagaa aatgaatcgc atacgcaaca aggtttacgg
     9661 atgagatggc ggaataaacg ctcggatcga atttcggcag gcttcttctc tttagcttgc
     9721 gtgcgccaga ggttttggct gtcatcaggg tttggttctc tttcgtcata tggctgtgcg
     9781 gcagtatatt acaccatttt ccacaaaatg cagtcagcgg acaggttatc tgtaaatggt
     9841 atcatatggt tagagccgcg aaaggatacc cttatgatga ataaccgtca acttgccgat
     9901 acatttaccc tgatcgccaa tttatgtgag atcaaaggcg aggtcattta tgtcattctc
     9961 gcctatcgca aagcctccga aaatttaatg accctcgggc gcgaagccag cgaatattgg
    10021 aaagagggga aactgcgcga gatccccggc gtgggcaaag ccatcgcaga aaaaatagat
    10081 gagttgctca ccacgggcaa actgcaattc cttgaaaacc tcaaagagga agtccctgct
    10141 tccttggcag attggctgca agtgcccggg ctgggaccga aaaagatcgc cctgatctgg
    10201 aagacgttaa acatcaccgc tctttctgaa ctggaaactg ccgccaaaaa cggacagctt
    10261 cgcggactcc cgggcatggg tgccaagtcc gagagcgcca ttctggaagg cattgcatcc
    10321 cttgcccgca ggtccgggcg gattccgctt gggcgggcgt atccgctggc gcaggagatc
    10381 atcaggactc tgaagcgggt ggatggagtc gtcgcagcag aatccgctgg cagtttaaga
    10441 aggatgcgtt ccacggtcgg cgatctcgat attctggtgg catccacaaa ttccgcggcg
    10501 gtgatggaag cgttcaccaa tctaccgggc gtgagtcgtg tgcttggcaa aggcgaaaca
    10561 aaatcgagca tcgaatttac cgatggagtc cgcgcgcagg tttgggtgca tgcgcccgaa
    10621 aagttcggca cggcgctgca atatgccaca ggctcgaaag atcacaacgt gcagctgcgt
    10681 caaattgctt tggctaaggg gctgtcgctt tcggagcatt cgctgacgaa gacgaatggc
    10741 aagggagaaa ttttctgcgc caccgaagag gaagtgtacg agagacttgg cttgccgtgg
    10801 attccgcctg agttgcgcga agatcacggg gaagtggtgg cggcaaaggc aggcagcctg
    10861 ccgaaattga ttcaggtcaa agacatcaaa gccgatctgc aaatgcactc gaattggagc
    10921 gatggaaaat taaccatgct cgagatggcg caggcagcga tcgcacatgg tttgaaggtc
    10981 atcgccttca cagaccattc tgccagcctc ggcgtgacgg gcggcttgaa aatggaagac
    11041 cacaaaaagc aagcggcaga gatcaagaag gtccaaaaac aactcggcga caagatcctg
    11101 attttgcacg caagcgaagt ggagatcaaa gccgatggca cgctcgatta tcccgatgag
    11161 tttctcgctt cgctcgatct ggtgctggct tcgatgcaca cctctctgcg tcagccgcgc
    11221 gagaaagtga ccgagcgcat gatcgccgcg attcgcaatc cgcatgtgga tatcatcggt
    11281 catcccacgg gacggctgat tcccgaccgt gagggcgccg acctcgatat ggaagccgtg
    11341 ctcaatgccg ccgctgagac aggcgtggcg ctcgagatca acgcgcatcc atcgcgcctc
    11401 gacctcgatg acatgtacgc ccgccgcgca aaggaactgg gcatccccat cagcatcaac
    11461 accgattcac attccgctga agatttcgac aatctctttt acggggttgc cacggcgcgc
    11521 cgcgcctggc tcacgaagga agatgtcatt aatacatggt ccacgaaaaa actgttgaat
    11581 tggctggaga agcgcggaaa atgaaagccc tgctcaagcc ttcgttaacg aagatattgc
    11641 tcgctctggg tttatttatc gttgccagtt ttttgtggaa atataccttt ggccttgtca
    11701 tcatggatgt gaactatttt ggatggccct tgcattactt gacaacctgg ggaccatgca
    11761 gggcagggga gaattgttcc gaattcaacg ggatgtacct ggttttcgat ttcctgtttt
    11821 ggtatatgat tggcgcatgg atcgtgaatc ggttttcgaa aaaaacaacc tagcattgga
    11881 ggcagaatgg cagaaagttt aataccggca aatgaagtcc gcttttttag cgggagttca
    11941 aatccgcaat tggcggggaa gattgcactg catttggggg tgccgctgga agatactcac
    12001 atctcacgtt tcagcaacga taatctttat atccaactgg gggccagtgt gcgctatcgc
    12061 cgtgtgtaca tcgtgcaatc gctctcaaag ccggtcaacg atcatttgat ggaattgatg
    12121 atgatgatcg atattgcgcg cagtgcagcg gcttccgagg tccatgccat cattccctat
    12181 ttttcctttg gccgttccga taaaaaagac gcgccccgca tttccatcac cgcccgcctt
    12241 gtggcagatc tattaaagac cgcaggagcc acgcatgtga tgagcatcat gttccactca
    12301 ccacaggtgc atggtttctt cggcatccct actgacccgc taagcagcag caaggttttc
    12361 aaagaatatc tttcaaaatc cgacctgagc ggctcgatcc ttgttgcgcc agatatgggg
    12421 caggccaaga ccgccgcccg cttcgcgcgt actcttaacc tgcccattgc ggcaggcaat
    12481 aaagagcgcg tttccgatac caaggtcatc atcaatggat tggtgggcga tcaggtacgc
    12541 ggtcatgaac gtgccctcat ctacgacgat gagatcgcca caggtggctc catccttgaa
    12601 ttaagccgtg tcttgatcag cgaaggactc aaagacatcc gcgtgatctg cacccatggt
    12661 gtcttcagca acaatggact cgagcgtctg gctgaagtcc ctgagatcac cgagatcatc
    12721 accacagata ctgtccatat cgaacctgag cgagtccacc cgaagttgac cgtgctttcc
    12781 gtggcgcctg tctttgccaa cgccattcgt cacaacatca atcgcgaatc gcttagcgac
    12841 cttttcgtgt acggcgaata nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 ctgtcgaaag tggatcggcc ccctgaaatt agttttcttt aacctgaaag atgtatactg
    13021 taatcaagga ggcacgacat gataaacaaa acgatgcaag atgcaatgaa cgaccagatc
    13081 aacaaggagt tattctcctc ttacttatat ctctccatgg cggcttattt tgaagataag
    13141 aacctgcctg gctttgccca ttggatgcgc atacaggaag ccgaagaacg cgaacatgcc
    13201 atgaagtttt acgatttcat ccttgagcgc ggcggacggg tgatgttgaa agccatcgaa
    13261 gctccaaaga ccgattggaa atcagctttg gaagttgcgg aagaggtggc ggcgcatgag
    13321 gccaaagtca cagcctcgat ccatgctttg tatgagttgg cgctaaaaga gaaggattat
    13381 cctgcccagg tgatgttgca atggttcatc tctgaacagg tggaagaaga gaagaatgcc
    13441 gctgaaattg tggcaaacct caagttgatc gaggaacgcg gcaccgccgt attgatgctc
    13501 gatcaccgcc tcggaaaacg cggcggcgag tagttcgaat ccgcccctaa agactgtctg
    13561 gcctttaggg gctttttatt tttggagacc catgcaaatc acgattcgtc gtgccaccca
    13621 tgccgataaa ggggaatggt ttcaaatgcg caagggcatc tggcccgatg tgcccgatca
    13681 ttatatgagt tttgatatgg atgacctgct tgccagcgag aattatctgg tgttatttgc
    13741 ctgtgatggg gaaactccca ttggcctgac cgaagcgaga ttaagagacc atgctgaagg
    13801 atgcagcacc agccctattg ggtatattga aagctggtat gtacgggagg attaccgcgg
    13861 caggggagtg accggaataa tgaatcaggc ggctgaagag tgggcccgct caaaaggctg
    13921 caccgaaatg gcttccgata cttggctgga taacgaagcc agcatccgcg cgcacttgaa
    13981 actgggatat ttcgaggtgg agcggctggt ccattttgtg aaaaaactgt agcgggtgcc
    14041 cgtatttgtg gtgaaatcac ttgtgggaaa aaagtccttg ccgcgacggg cgtgcgagga
    14101 tgtaaaatag aggaaactgc cgctaaaaca agatgcaggt aaacggtcgg gttggatgcg
    14161 tgaagagtgg ggaaatcctg ctctggattg tagtcatctt gcagtttggc atctgaatgg
    14221 ttcctgttcc aataaatctg tataatggtt gcgtgacctt gtaaatttta tgagcagcat
    14281 acgtcttaga gtcgtcattc cgtacctgtt gttgaccctg atcgtttccg ggatcggggt
    14341 gtatgtcgtc acgaatctcg tttccaatac gttgagcgag cgtctggcaa atcagttggt
    14401 gcaatcaggc cgtgtggtgt tggatggttt tgtaaaacag gaacagtctc agatcgagga
    14461 tgcgcgtttt attgccttta cttcgggtgt ggcggatgcg cttgtaagcg gtacggctga
    14521 cgggcttttg gtcgaacagg ccaccacgca aatgacaggg cgtgatattg atacgcttgt
    14581 gttcatttca tcgcaaggaa atgagatctt gcatttatgg aagaacgacc tgaatgtggt
    14641 tcagcgggcc aatgaaaatt cgggcatggc tggttcgccg ctggtcacgc cgtttttagc
    14701 cagcagaaac acccgcgata atccacggca ctttttgggg accaactcgc tgaatgaccg
    14761 cacttatttt tatgcttcca ttcccgcttc gttgaatggt gaatttgcgg gtgtggtggt
    14821 gatcggtatc gatattgaga agttgctgcc gaatttaaaa agctcggctt tggccgatgt
    14881 ggttgtctac ggcgcggatg ggagtgtcat tgccaccacg cttgatgtca gcgacccttt
    14941 gagtttggag gcgctttcga tcaccaacga taattaccag cgcatcagcg ccctgaccga
    15001 tctggtgacg gggagcaaca tttcgattga caggcgttcg tatagtgtgg cgcgtggttc
    15061 tttgcaattg gggaacgata aattgggggt gtttgctgtg gcgttgccgt tggattttgt
    15121 caatcaggcg gtttctgaaa atcagaccac ctacacactt ttgttcatcg gcgttttctt
    15181 tttcgtaatt gtgatcggtc tttgggtatc gcgctcgatc gtgaatccgt tgtatcaatt
    15241 ggtggaaact tcgcaggcca tttctgcggg tgacctcgaa aaacgcacgg gcatcaaatc
    15301 cagggatgag atcggtattc tcgcctcggc ttttgatgag atgaccgcga acctgcagga
    15361 gcgcaacgcc caactggaac gtgcaaacca gattttgaag aatattgata aaacgaagtc
    15421 caatttcatt cagatctccg cccatgagtt gcgtactcca ttgacgctta tcatggggta
    15481 ttcacaaatg ctggagcagg atgcccgcaa cgacccagaa ttaaagtcac tggcgcaggg
    15541 gattttagac ggttccgaac gcatgaccag cattgtggaa agcatgctgg atgtttcccg
    15601 cattgacagc aattcccttt tccttagaaa gaccggcctg cggattgacc tgatcgttcg
    15661 gaaggtttac aaaaattttg aaaaggcctt ggtcgagcga gatttgcaat ttgacatgaa
    15721 caacttgcgg gatctgccgc ttatccctgc cgacccggat atgctgcaaa aagtgtttta
    15781 tcatgtcatt atgaatgcga tcaaattcac cccggatggc ggttccatta cgataagtgg
    15841 acggtatgtg cccgccaatg agcccagtgt ggaaatttcc attcgcgata cgggtatcgg
    15901 catcaaccct gaaatgcatt ccctgatctt tgagaaattc aatcagacgg gtgacgtact
    15961 tttgcactct tccgggaaga caaaattcaa aggcggcgga ccgggcctgg gcttggcaat
    16021 tgcgcgtggt atcgtagagg cgcacggcgg ccgcatttgg gttgaaagcc cgggctataa
    16081 cgaagaaacc aatccaggca gtacattcca tatttccctg cctgctcccg aagaccaaca
    16141 agaggaggtg cgccgtgaac cagcgatctc caaacaatag acgtgacctt aacttatttg
    16201 cgatcattat ctccattgga gtggctttga tgatcggtgc cggacaactg gcagcgcggc
    16261 tcccatcgac ctggagcctg tctgccaata tgcgctccaa ccttacccca aactttttct
    16321 cgcgacgacc tgagaagact ctgcctgctg tggaagaagg tgcgggcgaa aagcaggatg
    16381 atacttatct tacgcccggc actggcactg cttctggttc ttcttccggg ccgatcaatc
    16441 cgaacggttc ggcaaccttc acccaacgcc cgcccgctac tgccacttcc acccagcgtt
    16501 ctgctccgac agccacacgt acttctccgc ccactgcaac cagtacgaga cgttccacag
    16561 tcgtggctgt tacatccacg cggactgcaa cacgtacccc gattcggact tccacctctg
    16621 tgtctcgaac cccaaccatg acggccactc gtaccgccac atctttattt actgccacca
    16681 gaaccgccac cccaacaagg acagccactt caacttcgac agcaaccgtt acgcctacgt
    16741 caacgcccac tgcttctgcc acagccacat ttacggtcat accgaccgct acaaatacgg
    16801 ctacgttcac cgcaacggca acgaatacag ctacttcgac ggcaacagct acaaatacac
    16861 catctccaac tgcaaccttc acagcaactt caaccttcac agcgactccg accagtaccc
    16921 cggtcttctc tggaacacct acaaccgtcc cgccagaatt agtggttggc actccagatg
    16981 gtaatgcggg ttatattccg aatgagaatt atgtgattgt tgacatgact tcgaatccga
    17041 tcgaagtaaa aaatccagca gatggatttt atgatttcat cttttatgag ttgtttgttg
    17101 ctccagatcg aatcaacatg gacagaatca ttttgagcat ttcaatggat ggaactaatt
    17161 attacgaagt gttcaactgg ggagataata tccccgacaa caatacgaat atttacactt
    17221 acactccaga aaacgataac cttccgattt tcacaacggt tctttatggc gtacccctat
    17281 tgcagacagg tatcgtggtt gatgtggata atgtagcaag taatccacca cccggcttgt
    17341 acatttatct ggcgattgag gtgccagctg ggcctattaa tgatggagcc ggtattgatg
    17401 ccattcaggt tcaggaagtt ccaataccct tcccgtaaaa aatgaaaaaa cattaatcaa
    17461 tacaaactca ct
//
LOCUS       DAOQJC010000087        17420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1544_length_17420_cov_10.091045, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000087 DAOQJC010000000
VERSION     DAOQJC010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 17420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17420
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1544_length_17420_cov_10.091045"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1720
                     /locus_tag="PLE39_13110"
     CDS             110..1720
                     /locus_tag="PLE39_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69316.1"
                     /translation="MLTEKTRIFLKENWIIVLLSALFGMWALAFIYHSSYVAIDDRRY
                     FNLFDDAMISMRYAWNFSHGHGLVWNAGGAERVEGYTNLLMTLMMSVATFLFEKRYAV
                     LAVQLTGIFFVLGSAFLALQIFHTINPNRQPAAKYFLFAAILLYYPLNYWSLMGMETG
                     MLAFLLGAGVFFSLQYANSQPLTESLKRRLPPIFPMSVLFGLAFLTRNDSLLFAALAF
                     LFLIPTLKNGHRSISIFFAAGFLYSAFVIGQTVFRYFYYGEIVPNTYTLKLVGMPLVD
                     RLQNGWGFIQPFLKETSFIFLLALIGVLLKFSFQKLYLLAIFLTSVLYQIYVGGDPWN
                     YWRIMAPTMPFLFILFFEGCAEALRFFPKLKTAPASSIIIFTLALCGLSLADSRFLRE
                     MTLSALPHDNGATHKQTDIAIAINELTDEKATIGVFWAGTIPYYTDRPAIDFLGKSDP
                     YIANLPPDLSGQMGWLGMSSVPGHNKYDLNYSIKILMPTYVQEFEVGSQNLNDWGAEH
                     YVNVKYHGIRLYLLKDSAYVDWEKARQR"
     gene            1764..>2147
                     /locus_tag="PLE39_13115"
     CDS             1764..>2147
                     /locus_tag="PLE39_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69317.1"
                     /translation="MNEPRVYKHSPIQFIILIVVMGFVFVAMFFNLDWNTPSILFPIS
                     LIILIAVAGLAAATQKTILSDDEISSQSLFGMKTLRWSEITRVSGRGYVIKLHNFDGD
                     VTVAPSPNLPRYEEVIEIIGNKRPDL"
     assembly_gap    2148..2247
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2248..2755
                     /locus_tag="PLE39_13120"
     CDS             <2248..2755
                     /locus_tag="PLE39_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69318.1"
                     /translation="LPRYEEVIEIIGNKRPDLFNPREYDELKSGSFFLLAGLAFLVLF
                     LSMIAGVGYFLYSSPDTARLGSVIFAPLIVLFIVMVVFAGTIVFRPRSLTLDGKSMTI
                     KYLFREKTLLADEIRSVQFAYQQSRNGKIFFVLLHLVNRKSLRLSGLNPNLPVVYLVL
                     KNWHQQNG"
     gene            3057..4685
                     /locus_tag="PLE39_13125"
     CDS             3057..4685
                     /locus_tag="PLE39_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HNE69319.1"
                     /translation="MTQHRITDDLDALLDVLPTSIRHAVEKANNSDKLLEIVIDLGRV
                     PTARFVEGEIVLSEKEITRAEIDHITERIGTFDADNRAGMERTLHRISAIRNRRSAIV
                     GLTCRVGRAVYGTVDIIQDIIESGKSVLILGRPGVGKTTLLREAARILAENKRVVIVD
                     TSNEIGGDGDVPHPAVGKARRMQVREPMLQHEVMIEAVENHNPEVIVIDEIGRELEAL
                     AARTIAERGVQLIGTAHGQTLDNLLLNPTLSDLVGGIEAVTLSDEEARRRGTQKTVLE
                     RRAPPTFDVLIEIQHRERFAVHLDIMSAVDSLLRDVPMPPEIRTRDEAGQIQIEKAAP
                     TKPAQKADSKLPRRGRGTTDQTTGRPAPVIQEQTQRSDSINPPVIGTPSVGARLQTVR
                     VFAYGVARNRLMQAAKRIGVPAVVVADVSEADVLVTLRTYYRNREKTVIDAESRGMPI
                     YVLRANTVAQVEQFLGDLYNLTEHQAPRDNMEDIRGQTEQAISAVLNGERWVDLPPGP
                     SLVRRLQHEMARNAELVSHSYGKEPRRHVRIFRE"
     gene            4792..5412
                     /gene="tmk"
                     /locus_tag="PLE39_13130"
     CDS             4792..5412
                     /gene="tmk"
                     /locus_tag="PLE39_13130"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNE69320.1"
                     /translation="MFITLEGPEGSGKTSHIPYLVEYLREQGYVVFPTREPGGTSISE
                     QIRDILHDLKNAEMHPRTETLLYQAARAQIVEQVIQPRLSAGEIVISDRYFDSTIAYQ
                     GYGHQQNLDEVRALVKYATGGLTPDLTVLLDLDVEVGLARKKGDKDWNRMDAKTVEFH
                     KRVRAGYLEMVKAEPKRWIVVNSDQSWETVQEGLKRVIVGRLQGEG"
     gene            5418..6104
                     /locus_tag="PLE39_13135"
     CDS             5418..6104
                     /locus_tag="PLE39_13135"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HNE69321.1"
                     /translation="MPLNLHLLSTPGRGDISDVIEASRPYLEDKRDPLVAYLPLGSLY
                     AERWLELNETAFKGLARLQMVNTELMRQKEIEDILRDASLVYIPGGNTFLLNHRLHLN
                     GIALYLKKKIQAGLPLVGFSAGAILCGPNILTSKDLNTVGTTFFDGLGAVPFNISPHY
                     PLDLHGQSVKDEWLADYHFFHDNPIVMLSDGAHIKVDGKKATLVSGEAWIWRKDEDKE
                     QLEEGKLIVG"
     gene            6114..6620
                     /locus_tag="PLE39_13140"
     CDS             6114..6620
                     /locus_tag="PLE39_13140"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HNE69322.1"
                     /translation="MDEQWMPGSKKELMSAIEREWKLLLQLAENLTDAQMTRPDPGGW
                     TPKDNLAHLTEWMNVLIGVHLDKRPAHEVLGVAANVTESWDMELINPVLFERNKDRSR
                     QDVLDGLKKTYGMVYARLDAMSFEDLLAPRHATDPEKRPILLWVLGDTTEHFLEHRET
                     IERGLKAG"
     gene            6623..7369
                     /gene="map"
                     /locus_tag="PLE39_13145"
     CDS             6623..7369
                     /gene="map"
                     /locus_tag="PLE39_13145"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNE69323.1"
                     /translation="MIVESEKDINSLKAIGRVVAQALKLMMEQARPGMTTAELDAIGG
                     AFLEKEGARSAPQVMYSFPGANCISVSPVIAHGIPGDHVLREGELINIDVSAEMGGYF
                     ADTGGSMVIAKHVPVYEKMIEATKSALVKAVQAAKAGQPLNGIGKAIQDEARRNGFGV
                     IFDLTGHGIGHKLHEEPSQVYNYFKSKDRRVLEEGVVLAIEPFLTTGKGHVLEEADGW
                     SLRTIDRTIAAQFEHTVIVTKDEPIILTLA"
     assembly_gap    7396..7495
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7502..8083)
                     /locus_tag="PLE39_13150"
     CDS             complement(7502..8083)
                     /locus_tag="PLE39_13150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNE69324.1"
                     /translation="MKSICVFCGSSDSVHADYKTAAFQMGSTLARRGIRLIYGGGKTG
                     LMGEVANGALSAGGEVIGIIIPSMNTPALAHTSLTRLDVAPDMHGRKARMHELSDGYI
                     ALPGGFGTWDELFETITWAQTGAHEKPVGLLNVKKYYAPLLAAMDHAVQEGFVFEEHR
                     KAVFCEMTPETLLDEMSKYQHPRDAVKRWLKED"
     gene            8182..9078
                     /locus_tag="PLE39_13155"
     CDS             8182..9078
                     /locus_tag="PLE39_13155"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNE69325.1"
                     /translation="MEYKDYYKTLGVDRKASVDDIRKAYRKLAMQYHPDKNPGDKKAE
                     DKFKEINEAYQVLSDEQKRARYDQLGSAYSNFRTSGGRPGDFRWDDWFQQQQGTQQRG
                     YGTEDIFGGAGGFSDFFRTIFGEAMRSSGREQSAEQGYQQEAEITFKEAYSGTLRQIQ
                     TNGRTLQVRIPAGVKTGSKVRVAGAGPDGLDLYLVVNIIDGGQFERDGQDLYTTSSVN
                     VLTLIIGGEAEVETPTGKVMLNIPAGTQVDQVFRLAGRGMPSLKTPHTNGDLFVKLKP
                     LVPKYLSPKQRELLEEAARIKF"
     gene            9100..10233
                     /locus_tag="PLE39_13160"
     CDS             9100..10233
                     /locus_tag="PLE39_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNE69326.1"
                     /translation="MKTKRSIAAFVVLVLAMLACQMPALPVQQAASPSEPVLPVSPLN
                     SDTANPVTDADGLVALFENVSPGTVAIITDVGQGSGFVYDGNGHIITNYHVVEGASTI
                     EVRFTSGYMAYGTLIGTDLDSDIVVIKVDAPAGELHPLPLGDSDSLKVGQTVVAIGNP
                     FGLDSTMTVGIVSALGRTLDAIRETPEGRPFTAGDIIQTDAAINPGNSGGPLFNINGE
                     VIGINRAIRTTSTTETGQPVNSGIGFAISINMVKRVVPVLIETGQYDYPFLGISSLDS
                     LSLDMINELGLKSFTGAYITDVVSGGPADKAGLIAGDTPTAIPDLFSGGDLIIAIDGR
                     DIRTFDEMLAYLITHKSPGETVILTVLRGEEKVDVSVELGKRP"
     gene            10350..12077
                     /locus_tag="PLE39_13165"
     CDS             10350..12077
                     /locus_tag="PLE39_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69327.1"
                     /translation="MKTFRVPLIRKILSLLALLMVLSLTGFVTYLCVQEFDVFTRILE
                     GRRSGFFVLFGLLVLVALTIKFVVYFMAVLNAQLIFDDKRMTIEAISGPGFLLWRGLK
                     PFSIELERVRSVFFNLLGMLEIIDAQGKRYAFSPGLFSMGSGEDILLELKSRVPPRAL
                     APILENSENFKHTHNWVFIRSLAFPVILMLYLLTMYLEPPNSELIHVWKSEMRLSWLE
                     DVEGYSVDPSGGFWVLSDTFAGYRVYRFLSSPTQSWKWNLEPFETQYPKFVSGDAEGT
                     PVIWLENGMMRYVNKKWEIMPYKENLVLESDRMFEAITEGHVLVLNGNAGQLLNINVS
                     NGEWYALTLPQDARQNRFVPASIKRTVDGEILVLMKNDRESRVYLLSNGEWSSQEYTI
                     IQADGRVRDYFLDEDGVLWVLLSIRGQSIVERLDNEGNVSVTRLPSMINKVDAASYSS
                     LRVDSSGRLWVEGKYPRFISVFAPVWGGDVDEIIRYSVENSNYQSGISSSTVLMPDGK
                     LWAFEKQVVSMDTNLNDLPAPLPDWFESLDLGVVRLILMMIFNILLITPSVVPMIYSL
                     SFPVRKQKL"
     gene            complement(12127..12768)
                     /locus_tag="PLE39_13170"
     CDS             complement(12127..12768)
                     /locus_tag="PLE39_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HNE69328.1"
                     /translation="MFAFEGFDRRKEEDRVRAEGRLPPGQSLTQKFPVLHYGPVPAFD
                     PATWDFRVWGEVEEEKRWSWDEFNKLPRSRIDMDIHCVTRWSKFDTTWEGVSVKTLVE
                     QGFFKIKPNATHVMQHAEYGFTVNLPLEVVLQENFLLATHFMGEPITPDHGYPLRGVV
                     GFIPGREDMETPYFWKGAKWLRSIEFMPRDKRGFWEQAGYHNRADVWREERFG"
     gene            12963..14870
                     /locus_tag="PLE39_13175"
     CDS             12963..14870
                     /locus_tag="PLE39_13175"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNE69329.1"
                     /translation="MKAPTFKQRFQYWFDNYMSQGTGALIGGLAVVSVVIISIAALII
                     AIGGRFLAPEGAEPPGFVEAFWLSLMRTLDAGTMGGDAGWGFRLMMLLVTIGGIFIVS
                     TLIGVLSNGLEDRLEELRKGRSVVLESDHTLILGWTPQIFTVISELVLANESRKSGAV
                     IVVLADQDKVEMEDAIAERIPDTKNTRVICRSGNPIDLTDLEIASPHTARSIIILAEG
                     ADPDTHVIKSVLAITNNPNRREEPYHIVTQIRDPKNMDVVKMLGVKDTVQPILTNDLI
                     ARVVAQTSRQSGLSIVYTELMNFGGDEIYFKQEPELSGKTYGEALEAYETSTLMGVRK
                     ADGTIAMNPSMDSRIESGDQIFVIAEDDDKIKLTGIGRKQFEERLIQVSGAASRSKPE
                     KTLILGWNRSGATIIRELDNYVAKGSQVTIVSDIYELDKQIKLDSGALKNQKITVKEG
                     DIRDRELLESLEATEYDHVIVLAYNHLEQQEADAITLITLLHLRDIAERDSTPFSIIS
                     EMLDLRNRELAEAAKVDDFIVSEHLISLMMTQLSENSELLGVFTDMFDPEGAEIYLKP
                     ISDYVVTGEPVNFYTVTEAARRRGETAIGYRVMSESHDASKAYGVHTNPKKSERVSYS
                     PDDKVIVIAEG"
     gene            complement(14910..16151)
                     /locus_tag="PLE39_13180"
     CDS             complement(14910..16151)
                     /locus_tag="PLE39_13180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HNE69330.1"
                     /translation="MTKPVYDILLEKMTYGGEAMGRLPDGRALFVPFGLPGEKVRVDL
                     VEEKKGFARGALLEILQPSPERIAPKCKHFGVCGGCHYQHLPYEKQLAAKTEVLRDQL
                     QRIGKIENPPVRPMIPSPLEWNYRNHVQFHLTAEGRLGFIHAKGTSTLPIEECHLPEA
                     GIHAFWPDLQFESNEGVERVSLRSGQDGELMVVFESDTPETPELEIEADVSVVHLFED
                     HPVVIAGEDHLTIKVSNRDFRVSAPSFFQVNTKMAEAMVQHLVSKLPVNETTTLLDVY
                     CGAGLFSAFFAQKCKRVVGIETSASACEDFAFNLDEFENIELYEGAAEEILPALADQL
                     KDAACAIVDPPRAGIEKHALDALIAIKPQVIAYVSCDPSTLARDAARLIHGGYQLVEV
                     TPFDLFPQTYHIESISIFEKN"
     gene            complement(16236..16406)
                     /locus_tag="PLE39_13185"
     CDS             complement(16236..16406)
                     /locus_tag="PLE39_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69331.1"
                     /translation="MAPKNKRPARVSPAAPQQTAAEFNPDYTQVKKDLTRIGLLSGTF
                     FILLVALSFVIK"
     gene            complement(16419..17330)
                     /locus_tag="PLE39_13190"
     CDS             complement(16419..17330)
                     /locus_tag="PLE39_13190"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HNE69332.1"
                     /translation="MARKKAPEDLSVEELRRLLVEKRRGARKDRLEYFRRTGRVLSIS
                     PGPSEAESDRAAPIVDTAEAAESSPEQVPVNPRRKFMDRVLLGVEVLAVLGLIAVLLN
                     SFGMLRTLNTEVASALNPETVTPTPLVMAVVLPSGHTPPDAQGNTRPNEAEIPDHLRP
                     MVQSLVNLPVPTAAPDQAVRLQIPALNIDAPIVQGDGWEQLKKGVGQNIGSSNPGQDG
                     NVVLSAHNDVYGEIFRYLDRLAPGDQVIVYTQQRQYTYIVDRTILVEPTAVEVMASTG
                     TPTVTLISCYPYLVNKQRIVVFARLQN"
BASE COUNT         4063 a         4344 c         4512 g         4301 t
ORIGIN      
        1 gacgccaaat tgtatcttct gaaaggttca cccgatattg attgggataa agtgaccacc
       61 atccttgcct ggtgatggtc gagtatcaca ctacacaatt tgaaagatca tgttgacaga
      121 aaaaacacgt attttcttga aagagaattg gattatcgtt ttgctctccg ccctttttgg
      181 catgtgggcg ctggcattta tttatcattc ctcctacgta gccatagatg accgccgata
      241 cttcaacctc ttcgacgatg caatgatctc catgcgttac gcatggaatt tttcacacgg
      301 acatgggctg gtgtggaatg caggcggtgc ggagcgtgtg gaaggctaca ccaacctgct
      361 gatgaccctg atgatgtcgg tggcgacctt tctctttgaa aaacgatatg ctgttttggc
      421 ggttcaactg acgggcattt tcttcgtcct cggttcggcg tttttggcat tgcagatctt
      481 tcacaccatc aacccaaata gacaacccgc cgcaaaatat tttctctttg ccgccattct
      541 tttgtattat ccgctcaact actggtcgct catggggatg gaaacaggca tgctggcatt
      601 cctcctcggc gcaggtgtct tcttctccct tcaatatgcc aactcacagc cactcaccga
      661 atcgctgaag cgccgattgc cgccgatttt tcccatgtca gttttgttcg gcctggcatt
      721 tctcacccgc aacgactcgc tcctctttgc cgcgcttgcc tttttatttt tgatccccac
      781 tctcaaaaac gggcacagat cgatctccat ttttttcgcg gctggcttcc tgtattcagc
      841 ctttgtcatc gggcaaaccg tttttcgcta tttttattac ggcgagatcg ttcccaacac
      901 ctacaccctc aaactcgtcg gcatgccttt ggtcgatcgc ctgcaaaatg gctggggatt
      961 cattcaacca ttcctgaaag agaccagctt catttttctt cttgcgctca tcggggtgct
     1021 gctgaaattc tctttccaaa agctgtacct gcttgcgatt ttccttacct cggtgcttta
     1081 tcagatctat gtcggcggcg acccgtggaa ttactggcgc atcatggcgc cgaccatgcc
     1141 gtttttattc attctctttt tcgaaggatg cgctgaagcg ctgcgcttct tccccaaact
     1201 gaaaactgct ccagcgtcct ccatcatcat cttcaccctg gcattgtgcg gactttccct
     1261 cgcagactcc cgcttcctgc gcgagatgac cctttctgcc ctgcctcacg acaatggcgc
     1321 cacccacaaa caaaccgaca ttgccattgc catcaacgaa ttgaccgatg aaaaagccac
     1381 catcggagtc ttttgggcgg ggacgattcc ctactacact gaccgtcccg ccattgattt
     1441 cctcggcaaa tcagacccat atattgccaa cctgcccccc gacctttccg gtcaaatggg
     1501 ctggctcggc atgagcagcg tccccggcca taacaaatat gacctgaact actccatcaa
     1561 gatcctcatg cccacctatg tgcaggaatt cgaagtcggt tcacaaaacc tcaacgactg
     1621 gggtgccgag cattatgtca acgtaaaata tcatggcatc cgcctctacc ttttgaaaga
     1681 ttctgcgtat gtagattggg aaaaagcccg ccaacgctga tcgtttgtat tgcaattgaa
     1741 attcctatcg aggagatacc cccatgaatg agccgcgtgt gtataaacat tctccaattc
     1801 aatttatcat tctgatcgtg gtgatgggat ttgttttcgt cgccatgttc ttcaacctcg
     1861 attggaacac cccgtccatt ctttttccga tcagtctcat catattgatc gctgttgcag
     1921 gcctcgcggc tgcaacccaa aagaccatcc tctctgatga cgaaatttct tcacagagtc
     1981 tattcggcat gaaaaccctg cgctggagcg agatcacccg cgtctctggt cgtggctatg
     2041 tcatcaagtt acacaacttc gatggcgatg tgaccgttgc gcccagtccc aacctgcccc
     2101 gctatgaaga agtgatcgag atcatcggaa acaaacgccc cgacttgnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnncct gccccgctat gaagaagtga tcgagatcat
     2281 cggaaacaaa cgccccgact tgttcaaccc acgcgagtat gacgaattaa agagtggaag
     2341 ttttttcctg ttggcaggcc ttgccttcct ggttttattt ctcagcatga ttgcaggggt
     2401 tgggtatttc ctgtacagtt cgcccgatac ggcccgttta ggctcggtta tctttgcacc
     2461 tctgatcgtt ttatttattg tcatggttgt atttgcaggc accattgttt tccgtccccg
     2521 ctcgctcact ttggatggaa aatccatgac catcaaatat cttttccgtg aaaagaccct
     2581 gcttgcagat gagattcgca gcgtccagtt tgcttatcaa caatcacgta acggaaaaat
     2641 atttttcgtt cttctccatc tggtaaatcg aaaatcgctc cgcctctcgg gcttgaaccc
     2701 caatctgcct gtggtgtacc ttgtgttgaa gaattggcac cagcaaaacg gctaaattcg
     2761 tcagacaaat caacgaaatt aacacatgcc tttaatttaa tgttaatcgc catcctgcta
     2821 taatatccct gcacgcgaca ccaatttaat gccgtggtaa tgggcacccc cggcacgtga
     2881 accgtgacac cctttaatag gcggtactcc aacatgctca accacaccct cctctaaccc
     2941 agcttccctg caacgtgttt cgcgcggatt tgcgcggcat accttatttc gtcttccctc
     3001 ccttttacca tcacgtgtca cttgatacct gacacttgca agctgaaact taacatatga
     3061 ctcaacacag aatcacagac gacctcgatg ctctcctcga tgtccttccc acttccattc
     3121 gtcatgcagt ggagaaggcc aacaatagtg acaaactcct ggagatcgtt attgaccttg
     3181 gcagagtgcc cactgcgcgc tttgtcgaag gtgaaattgt cctctctgaa aaagagatca
     3241 cccgcgctga gatcgaccat atcaccgagc gcatcggcac atttgatgcc gacaaccgcg
     3301 caggcatgga acgcacgctc catcgcatct ctgccattcg caacaggcgc agtgccattg
     3361 tcggcctgac ctgccgtgtg ggccgcgccg tgtacggcac tgtggatatc attcaagaca
     3421 tcatcgaatc gggtaaatcc gttttgatcc tgggccgccc tggcgtgggc aaaaccacgc
     3481 tgctgcgtga agccgcccgc atcctcgctg aaaacaaacg cgtggtcatc gttgatacat
     3541 ccaatgagat cggcggcgat ggcgatgttc cacaccccgc tgtaggcaag gcgcgccgta
     3601 tgcaggtgcg tgaacccatg cttcagcatg aagtgatgat cgaagcggtc gaaaaccaca
     3661 accccgaagt catcgtcatt gacgagatcg ggcgcgaact tgaagccctt gccgcacgca
     3721 ccatcgccga gcgcggtgta caactcatcg gcacggcaca tggccagacc ctcgataacc
     3781 ttttgctcaa ccccacgttg agcgaccttg tcggtggaat cgaagcggtc acactttcag
     3841 acgaagaagc ccgtcgacgc ggcacccaga aaacggtcct cgaaagacgc gcgcccccaa
     3901 ccttcgatgt cctcatcgag atccagcacc gcgaacgctt cgcagtccat ctcgatatca
     3961 tgtcggcggt agattcactt ttgcgcgacg tgcccatgcc gcccgaaatt cgcacacgcg
     4021 atgaggcggg gcagatccag atcgaaaaag cagcgccgac caaacccgct caaaaagcgg
     4081 attcaaaact cccgcgccgc ggacggggaa ccaccgatca aacgacgggc aggcctgctc
     4141 ccgttatcca ggagcagact caacgctctg actcgatcaa cccgcccgtc attggcacac
     4201 cgagcgttgg cgcacgattg cagaccgtgc gggtatttgc ctatggcgta gcccgcaacc
     4261 gattgatgca agccgccaag cggatcggcg tgcccgctgt ggtggtggcc gatgtcagcg
     4321 aagcggatgt gctcgtcacc ctgcggacgt attaccgcaa tcgcgaaaaa accgtcattg
     4381 atgccgaatc gcgcggcatg cccatctatg tactgcgtgc gaacactgtc gcgcaggtgg
     4441 aacaattcct cggagatctt tataacctta ccgagcacca ggccccgcgc gataacatgg
     4501 aagacattcg cggccagacc gagcaggcca tctcagccgt gttgaacggt gagcgctggg
     4561 tagacctgcc gcccggacca tcattggtgc gccgcttgca acacgaaatg gcgcgcaatg
     4621 ccgagctcgt cagccactcg tacggcaagg aaccgcgcag gcatgtaagg attttcaggg
     4681 aatagaacat agtaaatagg gaatagggag cgggcttgtc ctgctcccta ttttttatgt
     4741 taccatttgc aacatcctat tcacgtaaac ctattcacta tttcctaaac catgttcatt
     4801 actcttgagg gccccgaagg ctctggcaaa acctcccaca tcccatacct cgtggagtat
     4861 ctccgcgagc agggatatgt tgtcttcccc acccgtgaac cgggcggcac ttccatcagc
     4921 gaacagatcc gcgatatttt gcacgacctg aagaacgctg agatgcatcc gcgtactgaa
     4981 accctgctct atcaggccgc ccgtgcgcag atcgttgagc aggtcatcca gccgcgtttg
     5041 agcgcggggg agatcgtcat ctccgaccga tattttgact caacgatcgc ctatcaaggc
     5101 tatggccatc agcagaatct ggatgaggtc cgcgcattgg tgaagtatgc aacgggcggg
     5161 ttgacccccg acctgacggt tctgcttgat ctggatgtgg aagtgggcct tgcccgcaaa
     5221 aaaggcgaca aagactggaa tcgcatggat gccaagaccg tcgaattcca taaacgtgtc
     5281 cgggccgggt atttggaaat ggttaaagcc gagccgaaac gctggatcgt ggtcaattca
     5341 gatcagagtt gggagaccgt acaggagggg ttgaagcggg tgatcgttgg aaggttacag
     5401 ggtgaagggt aaatatcatg cctcttaacc tccatctcct ttccacgcct ggcaggggtg
     5461 acatcagtga tgtgatcgaa gcgagccgcc cttatttgga agacaagcgt gatccgctcg
     5521 tggcctattt gccgttgggt tcgctgtacg ccgagcgctg gctggagttg aacgaaaccg
     5581 ccttcaaagg gttggcgcgt ttgcaaatgg tcaacaccga gttgatgcgc caaaaagaga
     5641 tcgaagatat tttgcgggat gcatcgctcg tctatattcc cggtggcaac acctttttgc
     5701 ttaaccatcg tctgcatctt aatggaatag cgctttatct gaagaagaaa attcaagcag
     5761 gcctgccatt ggttggattc agcgcaggcg ctattttgtg cggacccaat attttgacct
     5821 caaaagatct caacaccgtg gggacgacat ttttcgatgg cttgggcgcc gtgccgttca
     5881 acatctctcc acactaccca cttgatctgc acggccaatc agtaaaggat gaatggctgg
     5941 cagattatca cttcttccac gataacccca ttgtcatgct ttcggatggg gcgcacatca
     6001 aggtggatgg aaaaaaagcc accctcgtca gtggagaggc atggatctgg cgcaaggacg
     6061 aagacaaaga gcaacttgaa gaaggcaagc tgatcgttgg ttaggagacc atcatggatg
     6121 aacaatggat gcccggtagt aaaaaggaat taatgtctgc gatcgaacgt gagtggaaac
     6181 tgcttctgca actggcagag aatctcaccg atgcacagat gacccgtccc gacccgggcg
     6241 gctggacccc aaaagataat cttgcgcacc tcaccgagtg gatgaatgtt cttatcggcg
     6301 ttcaccttga taaacgcccc gcacacgaag ttttgggcgt ggctgcaaat gtgacagaga
     6361 gctgggacat ggaactcatc aatcccgtcc tttttgaacg caacaaggat cgctcgcggc
     6421 aggacgtgct ggatggtttg aagaaaacgt atggaatggt ttacgcacgg cttgatgcga
     6481 tgagtttcga agacctgctg gctccccgcc atgccactga ccctgagaaa cgtcccattt
     6541 tgttgtgggt attgggcgat acgacggaac atttccttga acaccgcgaa accattgagc
     6601 gcggacttaa agcaggttaa gcatgatcgt tgagtctgaa aaagatatta attcactcaa
     6661 agccattggg cgtgttgttg cgcaggcatt gaagctgatg atggagcagg cccgtcccgg
     6721 catgaccact gccgaattgg atgccattgg cggtgccttt cttgaaaagg agggcgcgcg
     6781 atctgcccca caggtgatgt attccttccc cggagcaaat tgcatcagcg tttcgccggt
     6841 cattgcacac ggcattcccg gtgaccatgt tttacgcgaa ggtgaattga tcaatatcga
     6901 tgtctctgcc gagatgggcg ggtattttgc agatacgggc ggctcgatgg tcattgcaaa
     6961 acatgtgccg gtgtatgaaa aaatgatcga agccaccaaa tctgctttgg tgaaggcggt
     7021 gcaggcggcg aaagccgggc agccgctcaa cggcattggg aaagcgatac aggatgaagc
     7081 cagacgaaac gggtttgggg tcatctttga tctgaccggg catggcatcg ggcataaact
     7141 gcatgaagaa ccctctcagg tatacaacta cttcaagtcg aaagatcgcc gcgtgttgga
     7201 ggaaggcgtg gttctggcca ttgaaccctt tctcaccaca ggcaagggac atgtcctcga
     7261 agaggcagac ggctggtcat tgcgcaccat tgaccgcacc attgctgctc aattcgagca
     7321 tacggtgatc gtcaccaaag acgagccgat cattttgaca ttggcatagg gagtggagcc
     7381 tgtaaataaa ttacannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtttt
     7501 cttaatcctc tttcaaccaa cgcttcacgg catcgcgtgg atgctggtat tttgacattt
     7561 catccagcag ggtctcgggg gtcatttcgc aaaacacagc cttgcgatgt tcttcaaaga
     7621 caaatccctc ctgtacggca tggtccattg cggccagcag gggggcgtaa tattttttca
     7681 catttaacag tccgaccggc ttttcatggg caccggtttg cgcccatgtg atggtttcga
     7741 agagttcatc ccaggtgcca aaaccgccgg gcagggcaat gtaaccgtcc gaaagttcat
     7801 gcatgcgggc tttgcgtcca tgcatgtcgg gggctacatc caggcgggtc aggctggtgt
     7861 gggccagtgc gggtgtgttc atggatggga tgatgatacc gatcacctcc ccgccagcag
     7921 ataatgcccc gtttgccact tcacccatca agccggtctt gccgccgcca taaataaggc
     7981 ggattcccct ccgggcgagg gtactgccca tttgaaacgc ggcagtcttg tagtccgcat
     8041 gaaccgagtc ggacgatcca caaaatacgc agatagattt cattttatta ttccttttgt
     8101 ctcaatcata acaggttaga gttgtgttag tgtgcgcttt gggtgcttga cgaggaaaag
     8161 gcgcggtgtc acaattgcgg catggaatac aaagattact acaaaaccct cggcgtggac
     8221 cgtaaagcca gtgtagacga tatccgtaag gcatatcgta agctggccat gcagtatcac
     8281 ccggataaga acccgggcga taaaaaggcc gaggataagt tcaaagagat caacgaagcc
     8341 tatcaggtat tgagcgacga gcaaaagcgc gcccgctacg accagttggg cagcgcgtat
     8401 tccaatttcc gtaccagcgg cggacgcccg ggcgatttcc gctgggatga ttggtttcag
     8461 cagcagcaag ggacccagca aaggggctat ggcacagaag atatctttgg cggtgcaggc
     8521 gggttctccg attttttccg caccattttt ggcgaggcga tgcgctcttc ggggcgggag
     8581 cagtctgcag agcaggggta tcagcaggaa gcggagatca ccttcaaaga agcctatagc
     8641 ggcacattgc gtcagattca gaccaatgga cgcacgttgc aggtccgcat ccctgccggg
     8701 gtgaagacag gttcaaaggt ccgtgtagca ggtgcgggtc cagatggact cgatctgtat
     8761 ctcgtagtga atatcattga tgggggacaa ttcgagcggg atggtcagga tctgtacacg
     8821 acatcctcag taaatgtgtt gaccttgatc atcggcggtg aagccgaagt tgaaaccccc
     8881 acaggcaagg tcatgttgaa catacctgcc ggcacccagg tggatcaggt attccgcctt
     8941 gcgggacgcg gtatgccgag ccttaagact ccgcatacaa atggtgacct gttcgtaaaa
     9001 ctgaagccat tggttccgaa gtacctttcg cccaaacaga gggaattgct tgaagaagca
     9061 gctcgtatca agttttaaag ttgaacatag gagtatttca tgaaaacaaa gcgaagtatc
     9121 gcagcttttg ttgtgttagt gttggccatg cttgcctgtc agatgcctgc cctgcctgtg
     9181 caacaggcgg ccagcccgtc tgagccggtt ctgccggtgt cgccgctcaa ttcagataca
     9241 gccaaccctg taaccgatgc agatggtttg gttgcgcttt ttgaaaatgt cagcccgggc
     9301 accgtggcca tcatcaccga tgtggggcag ggttcgggtt ttgtgtacga tggcaacggc
     9361 cacatcatta ccaactatca tgttgtggaa ggtgccagca ccattgaagt gcgtttcact
     9421 tccggttaca tggcctatgg cactttgatc ggaaccgatc tggattcaga cattgtggtc
     9481 attaaagtgg atgcgcctgc cggtgaactt caccccctgc cgctcggcga ttcagactca
     9541 ctgaaagttg gtcagaccgt agtagccatt ggcaacccct ttggcctcga cagcacaatg
     9601 acggtgggca ttgtctcggc ccttggccgc actttggatg cgatccgcga aaccccggaa
     9661 gggcgtcctt tcacggctgg ggatatcatt cagaccgatg cggcgatcaa cccgggcaat
     9721 tcgggtggtc cgcttttcaa tatcaatggt gaagtgatcg gcatcaatcg tgccatccgc
     9781 accaccagca ccaccgaaac aggacagccc gtcaattcgg gcattgggtt tgcgatttca
     9841 attaatatgg tcaagcgggt tgtaccggtg ctgatcgaga ccggacaata cgattatccc
     9901 ttccttggca tttcatcgct cgattcgcta agcctcgata tgatcaatga actggggttg
     9961 aaatccttca ccggtgccta catcacagac gtggtcagcg gcggcccggc ggataaggct
    10021 ggcttgatcg caggggacac cccaaccgcc attcccgatc tgttcagcgg cggagacctc
    10081 atcatcgcca ttgacgggcg cgacatccgt acttttgatg agatgctggc ctatctcatc
    10141 acccacaaaa gccccggcga aaccgtgatc ctcaccgttt tgcgcggcga agaaaaagtg
    10201 gatgtaagcg tggagttggg gaagagaccg taggaattga tccatgcagg agcacagccc
    10261 ggaaccatgg ttccgggctg tttttttgca accgggcgga tggagttgta taattcagac
    10321 aagttcaata tttcatttgg gagggtttta tgaaaacatt tcgtgtgccc ctgataagaa
    10381 aaatattaag tctgcttgcg ttgttgatgg tgctgtcgct gacgggattt gtgacctatc
    10441 tatgtgtcca ggaattcgat gtgtttacaa gaatcctgga aggcagacgt tctggatttt
    10501 ttgttctctt cggcttgctg gttttagttg ccttaacaat caaatttgtt gtttatttca
    10561 tggccgtgtt gaatgcacag ttgatttttg atgataaaag aatgacgatt gaagccattt
    10621 ccgggccggg gtttttgctt tggagagggt tgaaaccttt ttctatcgaa cttgaacggg
    10681 ttagaagtgt attttttaat ttattgggaa tgctggaaat cattgatgct caaggaaaaa
    10741 gatatgcttt ttctccaggt ttattcagca tggggagcgg tgaagatatc ctgttggaac
    10801 taaaaagccg tgtgccaccg agagcacttg ctcccatttt ggagaattct gaaaatttca
    10861 agcataccca taattgggtt ttcattagga gtttggcttt cccagtgatc ttaatgctgt
    10921 atttgcttac tatgtatttg gagccgccaa attcagagtt gatccatgtg tggaaatctg
    10981 aaatgcggct atcatggctg gaggatgtag aggggtattc agtggatccc tcgggtggtt
    11041 tttgggtgtt atctgatacg tttgccgggt atcgcgttta tcgttttttg agttcgccaa
    11101 cgcagagttg gaaatggaat ttggaaccat ttgaaaccca atatccaaaa tttgtcagcg
    11161 gggatgctga agggacaccg gtcatctggt tggaaaacgg aatgatgcga tatgtaaaca
    11221 aaaaatggga aatcatgccc tacaaggaaa atcttgtatt ggagtctgac agaatgtttg
    11281 aagccattac agaggggcat gtgctggtgt tgaatggaaa tgcagggcag cttttgaata
    11341 ttaatgtttc aaacggagaa tggtatgccc tgacacttcc acaggatgcg aggcagaatc
    11401 gatttgtgcc agcatccata aaaagaacag tggatggcga gattcttgtg ctgatgaaga
    11461 acgatagaga aagtcgggtg tacctgcttt caaatggtga atggagttcg caggaataca
    11521 ccattattca ggcagatggc cgtgtacgag attatttttt ggatgaggat ggtgtgctgt
    11581 gggttttgtt gtccatccgt ggtcagtcca ttgtagaaag attggataat gagggaaacg
    11641 tttccgtaac aagattacct tcgatgatta acaaagtaga tgcagcaagt tattcttctt
    11701 tacgtgttga ctcatctggg cggttatggg tcgaagggaa atatccaagg tttatttctg
    11761 tatttgctcc agtatgggga ggtgatgtgg atgaaattat tcgatactcg gtcgaaaatt
    11821 caaattatca atcaggtatt tcttcctcca cagttttgat gcctgatgga aagctttggg
    11881 cttttgaaaa gcaggttgtc agcatggata ccaatttgaa cgatttgcca gccccactcc
    11941 ctgattggtt cgaatctttg gatttgggcg tagtaagact gatcctgatg atgatattta
    12001 atattttgct cattacccct tctgtggttc ccatgattta cagtctatct ttccctgtaa
    12061 gaaaacagaa gttataacgc ccgcaaaaca aaaagggaca cggtatctcc gtgtccctgc
    12121 tgaaacctat ccaaaccgtt cttcgcgcca gacatcggca cggttgtgat acccggcttg
    12181 ttcccaaaat ccgcgtttgt cacggggcat gaattcgatg gaacgcagcc atttggcgcc
    12241 tttccagaaa tagggagttt ccatgtcttc acggccgggg ataaatccca ccacgccgcg
    12301 caatggataa ccgtggtcgg gggtgatcgg ttcacccatg aaatgcgtgg caagcaggaa
    12361 attctcctgc agcaccactt ccagcggcag gttgacggtg aagccgtatt cggcatgctg
    12421 catcacatgg gtggcattgg gcttgatttt gaaaaagccc tgttccacca gtgtttttac
    12481 cgaaacccct tcccatgtgg tgtcgaattt gctccaacgg gtcacacagt ggatgtccat
    12541 atcgattctg gaacgcggta atttgttgaa ttcatcccac gaccagcgtt tttcctcctc
    12601 cacttcgccc catacgcgga aatcccaggt ggcggggtcg aaggcgggga caggcccata
    12661 atgcaggacg gggaatttct gggtgagaga ttgccccggg ggaagccgac cctcggcacg
    12721 gactcggtct tcttctttac ggcggtcaaa accttcaaat gcaaacatgg cttacctcac
    12781 tttctaaatc ataaaagttg aacgattgcg gcattgcatt gaccttttga atttgacaga
    12841 tgttacctgt tgaaaatgat agttttctta atgatgccta gtatagatgt aatgaacttg
    12901 caggtcaatt ttttgacggg ctgtggttat aataggccaa aatttcggcg tgaggagaag
    12961 agatgaaggc acccactttt aaacaacggt ttcagtattg gttcgataat tacatgtcac
    13021 aggggactgg tgcattgatt ggaggattgg ctgttgtatc tgtggtcatt atttcgatcg
    13081 cggccctgat cattgccatt ggcggacgtt tcctggcgcc tgaaggtgca gaaccacccg
    13141 ggtttgttga agccttctgg ctcagcctca tgcgtaccct ggatgcaggc acaatgggcg
    13201 gcgatgcggg ctggggcttc cgcttgatga tgctgctcgt gaccatcggc ggtattttca
    13261 tcgtctccac cctgatcggt gttttgagca acggtttgga agaccgcctt gaagaactcc
    13321 gcaaggggcg ctctgttgtc ctcgaatcgg atcatacact catccttggc tggacacccc
    13381 aaattttcac agtcatctct gaattggtgc ttgcgaacga gagccgcaag tccggggcgg
    13441 tcattgttgt tttggccgat caggacaagg ttgagatgga agatgcgatc gcagagcgga
    13501 ttccagatac gaagaatacc cgtgtcatct gtcgttcggg aaatcccatt gatcttacag
    13561 accttgagat tgcgagcccg catacggcgc gctcgatcat catccttgct gaaggcgccg
    13621 acccggatac gcatgtcatt aaatcggtgc tggcgatcac caacaatccg aatcgccgcg
    13681 aagaacccta tcacattgtc acccagatcc gcgacccgaa gaacatggat gtggtcaaga
    13741 tgcttggggt aaaggacacc gttcagccca tcctcaccaa cgacttgatc gcccgtgtgg
    13801 tggcgcagac ctcacgccag agcggtttgt ccattgtgta taccgagttg atgaattttg
    13861 gcggcgatga aatttatttc aagcaggaac cagaactttc gggcaagacc tatggcgaag
    13921 cgctggaagc ctatgagacc tccacattga tgggcgttcg caaagcggat ggtaccatcg
    13981 ccatgaatcc atcaatggac tcccgcattg aatcgggaga ccagatcttc gtcattgcag
    14041 aagatgatga caagatcaaa cttacaggga ttggccgcaa acaatttgaa gaacgtctga
    14101 tccaggtttc cggagcagcc tcccgctcca agccggagaa gactctcatc ctgggttgga
    14161 atcgttccgg ggcaacgatc atccgtgagt tggataatta tgtagccaag gggtcacagg
    14221 ttacgatcgt ttcagacatt tatgagttgg ataaacagat caaacttgat agcggtgctt
    14281 tgaagaatca aaagatcacg gtgaaggaag gcgatattcg cgaccgcgaa ctgcttgaaa
    14341 gccttgaagc caccgaatac gatcatgtca ttgtgctggc gtacaaccac cttgaacagc
    14401 aggaggcaga tgccatcacc ctcattaccc tgttgcacct gcgtgatatt gccgaacgtg
    14461 attcgacccc gttctcgatc atcagtgaaa tgctcgacct gcgcaaccgt gaacttgccg
    14521 aagccgccaa ggtggatgat ttcattgtca gcgagcattt gatcagcttg atgatgacgc
    14581 agctttcaga aaactctgaa ttgcttggtg tctttaccga tatgttcgac cccgagggtg
    14641 cggagatcta cctcaagccg atcagcgatt atgtggtgac gggtgaacct gtcaactttt
    14701 acaccgttac tgaggccgca cgccgccgcg gtgaaaccgc catcggctat cgtgtgatga
    14761 gcgaaagcca tgatgccagc aaggcatacg gtgttcatac caacccgaag aaatctgaac
    14821 gggtcagtta ctcgcctgac gacaaggtca ttgtcatcgc agagggataa tcattttgta
    14881 gaggcacagc agcgctgtgc ctctactttt taattttttt caaagatcga aatggattcg
    14941 atgtgatagg tttgggggaa aagatcgaac ggtgtcactt ccaccaattg atacccccca
    15001 tgaataagcc gtgccgcatc ccgcgccagt gtggatgggt cgcaggatac ataggcgatg
    15061 acctgtggtt tgatggcgat cagggcatcc aatgcatgtt tttcgatgcc cgcacgcggt
    15121 gggtccacga tggcgcaggc ggcatccttg agttggtcgg ccagggcggg cagaatttct
    15181 tcagcggccc cttcgtagag ttcgatattt tcaaattcat cgagattgaa ggcgaaatct
    15241 tcacaggccg atgcagaggt ttcaatgccg accacacgtt tgcatttctg tgcaaaaaag
    15301 gcgctaaaca gacctgctcc acagtaaaca tccagcaggg tggtggtttc gttgacaggc
    15361 agttttgaaa cgaggtgttg gaccatggcc tccgccattt ttgtgttgac ctgaaagaaa
    15421 gatggcgccg aaacacggaa gtctcggttt gagactttga tggtcaaatg gtcttccccg
    15481 gcgatgacga ctgggtggtc ttcaaacaaa tgtacaacgg atacatctgc ttcgatttca
    15541 agctcgggtg tctctggtgt gtccgattcg aacacgacca ttaactcacc gtcctgtccg
    15601 ctgcggagtg aaacgcgctc cacgccttcg ttcgattcaa attgcaggtc aggccagaaa
    15661 gcatggatgc ctgcttccgg taaatgacat tcttcaatgg gcagggtgga ggtacccttt
    15721 gcatgaatga atccgagcct gccctctgcc gtgaggtgga attgcacatg gttgcggtag
    15781 ttccattcga gcggtgaggg aatcattggc ctgactggcg gattttcgat tttgccaata
    15841 cgctgtaact ggtcgcgcag gacttcagtt tttgcggcca gttgtttttc gtatggaagg
    15901 tgctggtaat gacacccgcc gcacacccca aagtgtttgc attttggggc aattctctcc
    15961 ggggaaggct gcaggatttc gagcagtgcc ccgcgtgcaa aacctttttt ctcctccacc
    16021 aggtccacac gcactttttc gccgggcagg ccgaagggta cgaacaaggc acggccatcg
    16081 ggaagccgcc ccatggcttc gccgccgtag gtcatttttt cgagcaggat atcgtacaca
    16141 ggtttggtca tggtttttga aggaaaaata aaaagaggaa aagcacggaa tccgtctttc
    16201 ctctttttgg gttttgccgc tccgtgatgc gaggattatt tgataacgaa ggaaagcgca
    16261 accaacagaa taaagaatgt gcctgaaagc agaccgatac gggtaaggtc cttcttcacc
    16321 tgggtgtaat cagggttgaa ttccgctgcc gtctgttggg gtgctgcggg tgatacacga
    16381 gccggtcttt tattttttgg tgccatttta tatttctcct aattttgtaa tcgcgcaaat
    16441 acaacaatgc gctgtttgtt gacaaggtac ggatagcacg agatcaatgt tacggtcggt
    16501 gtgccggtgg atgccatcac ctccaccgcg gttggttcaa ccagaatggt gcggtcgacg
    16561 atgtaggtat attgccgctg ctgtgtgtac acgatgacct ggtcaccggg cgcaagacgg
    16621 tcaagatatc ggaagatctc tccgtaaaca tcgttgtgag ccgaaagaac aacgttccca
    16681 tcctgacctg gatttgagga accgatattc tggccaacgc ctttttttag ctgttcccat
    16741 ccatcacctt gaacgatcgg ggcgtcaata ttaagcgccg ggatctgaag gcgtaccgcc
    16801 tgatctggtg cggcggtggg gacaggcagg ttcaccagtg actgcaccat ggggcgcagg
    16861 tggtcgggaa tttccgcttc gtttggccgc gtgttgccct gcgcatcggg tggggtatga
    16921 cctgatggta atacaacggc catgaccagc ggggtcgggg taacggtttc agggttgagc
    16981 gcagacgcca cttccgtgtt gagagtacgc aacatgccga agctgttcaa taatacggcg
    17041 atcaaaccaa ggacggcgag tacttccaca ccgagcaaga cgcgatccat gaatttccga
    17101 cgcgggttga ctggaacctg ctccggggag gattctgctg cttcggctgt atcaacaatc
    17161 ggtgcagcac gatcagactc cgcctcggat gggccgggcg aaattgagag gactcttccc
    17221 gtgcggcgga agtattccag tctgtctttg cgcgcaccac gtcgtttttc aaccagcagt
    17281 cggcgcaatt cctcgacgga tagatcttcg ggtgctttct tgcgggccat gtcggtggga
    17341 ttatacaggg gattgtcaat ttttgggaca ccttgtataa tggcaatcct tcgggcgggt
    17401 agctcagttg gttagagcac
//
LOCUS       DAOQJC010000088        17179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1577_length_17179_cov_10.787199, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000088 DAOQJC010000000
VERSION     DAOQJC010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 17179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17179
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1577_length_17179_cov_10.787199"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PLE39_13195"
     CDS             complement(<1..1027)
                     /locus_tag="PLE39_13195"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNE69333.1"
                     /translation="MNLDDLDLFKKIDTRNMLAQIDGLPNQLQTAWELGQTLPLPAFK
                     DIQTIVISAMGDSAVAAELVIASVSSSIRLPVALHRGYGLPAFASGPQTLVVCISHSG
                     NSEEVLDVFEAAQKNNCTVMVISTGGEIAMRAADKNIPAWKFDSNGVVDTAAVAYPFG
                     LLLALFARLGLIPDPASDVAEAVAMMKRSQQHITADVIAAKNPAKRYAGQLVGRWVTF
                     VGTENLAPVARRWKMQINQLAKAGANFEIIPESTHNTLIATINPNPTLNAHTMTLFMK
                     APSDHPRNKLRSDLMRQAFMLEALNTDVIEARGDSVIAHLWTLMIFGDYMAYYLAMAY
                     GADPSEED"
     gene            complement(1114..2598)
                     /locus_tag="PLE39_13200"
     CDS             complement(1114..2598)
                     /locus_tag="PLE39_13200"
                     /inference="COORDINATES: protein motif:HMM:NF012664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69334.1"
                     /translation="MAEKKPEEKKDEKKDEKPAPKDNLVETKHSVKIGGKTIKYTVTT
                     GTMVVKEEVWDKDKEAEVDKPRAQMFFVAYTLDGVKDRSKRPITFSFNGGPGSSSVWL
                     HMGVLGPRRVVLTDEGEMPPPPFKLTDNAFSILDETDLVFIDPMSTGYSRPVEGEKSR
                     EWHTFSKDIASVGDFIRLYSTRYNRWLSPKFLAGESYGTTRASGLSGYLQERHGMFLN
                     GLILISAVLDFTTIDFNLNNDLPYILFVPAYAATAWYHGKLSFKASLKEVVREAEEFA
                     KGEYATALLKGAALTPEERASVVEKLSRYTGISKEFIERTNLRIYDQHFFKELLRERG
                     KTVGRLDSRFTGIDRNGVTAMPDYDPLFAQVNGPYTATLNDYLRTELKFETDLPYEIL
                     SDKVFLNWSYEYFQNQYVNVAETLRASMTFNKYLKVFVANGYYDLGTPYFATEYTFDH
                     LGLDESLRKNISMGYYEAGHMMYVHIPSLKQLKKDLSKFIKDAS"
     gene            complement(2591..2965)
                     /locus_tag="PLE39_13205"
     CDS             complement(2591..2965)
                     /locus_tag="PLE39_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69335.1"
                     /translation="MEYLTAIFSSILPQLPSLFLHAVGLGFSISRRKEYPRTALAAGI
                     YFGLALLIGLATQVYVVLPMYMVDQGGSTQDIGSIFTTLTFICVPFRVVMEVALLYAI
                     FAPRNSNSSDPNQPIFMGDKNG"
     gene            complement(3060..4136)
                     /locus_tag="PLE39_13210"
     CDS             complement(3060..4136)
                     /locus_tag="PLE39_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69336.1"
                     /translation="MNEQNSPTIPPLLGSFSQRLLYWIFIPVAPIVNFSFVSAMLPLW
                     QTGKFSDYMLLMLSPEAAALTLPLLGYSVISYLMLISDEERQSESLVIRFGIYGGVLL
                     SFHYSLLTALSMDLSPVLFGFVFFIFLPLLIPKLRAWLQNEKVFRALIFAVFAILVLW
                     GIVAAVFDYSTSPVLLLVILVPMSAPFWCFFIALQAARWLWKYHEPKLTHAHGFGLFT
                     WLSAYGVALYYNITKMYELYATLPTQPPNCYIATAAANGHSAFVGSQEIRLENGISMR
                     VNRQLQNLKCAELTLMVIVPHLHKILRSIYDLFGKALARRIQNPLLADAAYLSLKPFE
                     WTAVAVLELIVPNWDDLASKIYVR"
     gene            complement(4133..4957)
                     /gene="rsmI"
                     /locus_tag="PLE39_13215"
     CDS             complement(4133..4957)
                     /gene="rsmI"
                     /locus_tag="PLE39_13215"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNE69337.1"
                     /translation="MGTLFLVATPIGNLEDISPRALRILKEVQLIAAEDTRHSGVLLK
                     HFGIETRLTSYFEHNKIHKLDQILSALAEGDVALISDAGTPAINDPGYELVQAALASN
                     FDVVPVPGPSAPIAALTVSGLPTDSFLYLGYLPHKSSERLGFVGQVSNLPYTLIFLET
                     PHRLLDSLEDILAVLGDRRICIAREMTKRYEEYWRGDISGALEYFKSKDPRGEFTLVV
                     EGKKKEESSKWEESELMDAIRKELQTGRSAKEISADLAGQSGWNKKDVYALVNLVK"
     gene            complement(5096..6574)
                     /locus_tag="PLE39_13220"
     CDS             complement(5096..6574)
                     /locus_tag="PLE39_13220"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNE69338.1"
                     /translation="MPIPSKIGRYEVKSELGRGGMATVYRAYDPSFDREVAIKVLPRE
                     MLHDPQFRGRFDRELKIVAGLEHPSIVPVYDVGEEDGQPYFVMRYMTGGSLSDQIALG
                     KISVQDTARIVERIAMGLHYAHKKGIVHRDLKPDNILFDNNGDPFISDFGIAKLTESS
                     SGSLTGSGVIGTPAYMSPEQAKGEPIDGRSDIYGLGVIIYQMLSGQQPYNADTPMGVV
                     VKHITDPVPEILNMIPSLPIEVDGIIKMCMAKDKTRRYANAVELAKAMNMVAFGNEGK
                     AGLSNTGFRTVLPGTSTLQSPRARTGLAVAGVVLLVVVVGGFLLRNQLFTQPEATSTP
                     TLTAVVFTSTVEEVPTEKPTLTSEPPTETVASPLPFAPACAEGITIPIPEVRETNKAC
                     VTKIPYTSVSIPEGAEFESLSPDMTCAIETTSDGKSIVACTGRQLYSYELKVCTPPVV
                     ANEDLNKCESGSLFEAANQCCVPTPKDGAGCTTFKIDLRSCQ"
     gene            complement(6694..7404)
                     /locus_tag="PLE39_13225"
     CDS             complement(6694..7404)
                     /locus_tag="PLE39_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69339.1"
                     /translation="MQAVTHYIPIFTTVISFSFTVALYKHWQRKPEARYLMWWTIGAF
                     CFGIGTLTESINTLVGWSVWNMKAWYISGALLGAFPLAQGTVYLLLKKKTADFLAWFF
                     VVYIAVASVAIFLAPVDMSLVDPARLTGKVMTWTWARMFSILPNTYAMIFLVGGAAWS
                     AIQYARKLDSGSRVLGNWLIAIGALLPGIGGSFTRFGYVEVLFVTEFIGISLIWAGYR
                     AMTQTRTVSIHTAQAAAD"
     gene            complement(7488..8105)
                     /locus_tag="PLE39_13230"
     CDS             complement(7488..8105)
                     /locus_tag="PLE39_13230"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE69340.1"
                     /translation="MTDEWLSLSDAAKILGVHPGTVRLWSDKGLLPTHKTQGGHRRYR
                     RSEISLWAESNHRSNAIEPEGMMNEVVKNVRLQISEGRLQAESWYQKLDDDARTQYRM
                     SARSLFHGLMNYVATHGAEAESESYAIGYEYASRAHRYSLNYVDAAKAFLFFRNTLIE
                     SVIKVYGEANVPSGQTAEMFQKMHVFTDEILISLLQTYQSLNASR"
     gene            complement(8108..9412)
                     /locus_tag="PLE39_13235"
     CDS             complement(8108..9412)
                     /locus_tag="PLE39_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCD family MFS transporter"
                     /protein_id="HNE69341.1"
                     /translation="MFIKRLQLALIHTAVAITLVPINSTLNRVMIFDLGVSKTLFTLL
                     AIFPYLLSPIQVAIGSFSDRHPIMGYRRTPYILAGLLLCVLGVAIAPQIAVLVSENFT
                     LGIMVGIFAFGLWGMGYNLSAVSYLSLASELSGEKGRGKTVATMFTIMVIGLIATGIS
                     LSRMVTVYDPETLSRAFLIVAASALTLGLLGLFKLEPPFGRSDQTPRSETYTVKEMTA
                     AITSNPVAKIFFAYLLLLLAAILGQDVLLEPFGAQAFGMSLEQTSRIVSISSTFTLVA
                     FIVAGVLEGRVKKKYIAQMGNLGALIGFLIIVVSGLTGSLTAFYLGITLLGFGTGIST
                     VANLSLMFDLTVPEKVGLYIGAWGFSNGLSRLVGLLMAGVVADLATQMTGSALSGYLV
                     VFSLEALMLLAAAVMLYRIDVGAFQKQAHEPSYVEKVALSVE"
     gene            complement(9580..10122)
                     /locus_tag="PLE39_13240"
     CDS             complement(9580..10122)
                     /locus_tag="PLE39_13240"
                     /inference="COORDINATES: protein motif:HMM:NF017022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChuX/HutX family heme-like substrate-binding
                     protein"
                     /protein_id="HNE69342.1"
                     /translation="MFQTIQPLNLDLTVVERQKTIREAYNTDRSQMTLMLAHQLRIPE
                     VEIVRALEGDSAHELDFSRWQEIVCAFETLGNVHVIVSNGATTIEAYGQFGNLTNTGG
                     FLNVRTKSLDMHIRSWELDSVFAFRKPSHLDGIETLSFQFFDRQGAAAFKVFLNFGGK
                     VPAPALVERYDALIRQFGKK"
     gene            10288..10986
                     /locus_tag="PLE39_13245"
     CDS             10288..10986
                     /locus_tag="PLE39_13245"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNE69343.1"
                     /translation="MSASSAMQRYAAEIYRLQQDHEQVPLSLLSTHVESSAQAIASMV
                     KRLQKNGYLVHEPYRGVRLTPTGEKIAMPSLRRHRLTEVFLVKVMKYDWATAHQLADT
                     FELGVNDMIEDRMDELAGRPTRCPHGEPIPTKDGVMPVVRDMSLIDVPSGSDCVISRV
                     RTHDFEKLTYIGELGLVPGTSFHLLSCAPFKGPLRLNMKPHDHVVGYELAASLWVEVT
                     KEGEGNKLPAQKNN"
     gene            complement(11061..11957)
                     /locus_tag="PLE39_13250"
     CDS             complement(11061..11957)
                     /locus_tag="PLE39_13250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE69344.1"
                     /translation="MKPDISICITNYNAKDFLRDCLRSIYGTTDTLTFEIIVVDNFSG
                     DGGPEMIRSEFPDVKLLTNEGNTGFTRPYNQAMRLAQGRHVVVLNPDTVIFPKAFAEL
                     VGFLDSHPEVGIVSPKVLNRDGTLQKQCRRSEARPWDAICYFSGLSRLFPQDKRFAGY
                     LMTYLPEDLTHEAEAVSGSCMVVRREVVEQVGYFDEDFFAYQEDSDYCRRTRLAGWKI
                     FYVPTAQITHYGGEGGSKVQPYRTIIEWHRSYYLYFRKHFAKDYFFLFNYLYYLMMLT
                     KLGYALFTNLFRRKKIVSTPKP"
     gene            complement(11954..12949)
                     /locus_tag="PLE39_13255"
     CDS             complement(11954..12949)
                     /locus_tag="PLE39_13255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE69345.1"
                     /translation="MNAPLISIVLVAWNSAAYMPRCFQHLLAQTVQDFEIIVVDNGSK
                     DDGCVGLQEKYPTLALRVERLETNHGFSFANNLGAQLATGKWLTLLNTDAFPEPDWLE
                     RLLEAAQMHPNAFFSSRQLQANTPELLDGEGDVYHVSGLAWRNGYGSRTYPLDETREV
                     FSACGAAAFFPREKFLSVGGFDEDYLSYHEDVDLGFRLRLHGLKCFVVPRAVVHHVGS
                     VTFGKKSDFSIYHGHRNLVWTYAKDMPSVLFWFYLPLHIFINVFFLFYFTAKGQGRTI
                     WRAKFDALRGLAPMLKKRGAIQRNRKASIAELHRAMSRNWLAPFLTFLKRNTENS"
     gene            complement(12995..14260)
                     /locus_tag="PLE39_13260"
     CDS             complement(12995..14260)
                     /locus_tag="PLE39_13260"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HNE69346.1"
                     /translation="MPEPLRRRLSMLSQRIFRPRSAPAMPEPAKTKMYEQYTKQVLAI
                     PDKTDDYVDLASDAYVPQAGDPLLLAYYLPQFHPTPENDEWWGRGITEWHNVAKAVPQ
                     YVGHYQPRLPGELGSYDLRLKENIARQIELAKSHGIHGFAYYFYWFDGRRLLDKPLDM
                     FLNNPDLDFPFCLCWANESWTRRFDGSSGEVIMYQNESVESYKSFIESIKPYAADKRY
                     IRVDGRPVLIVYRPSFVPDCSNVLAHWRAYCEKEGLGNPYIIAVKENTFDGDLLALGF
                     DAQSEFHPGTVFRYCKNITNEINFMRDDFGGIVLDYVDLVKNKQYFKFQAKKLHRAIM
                     PMWDNTPRRNNTPMIFEGASPALYKEWLIDILREARSSKELDAPFIFVNAWNEWGECA
                     YLEPDRKYGYAFLRATREAIEETRKAEGE"
     gene            complement(14308..17082)
                     /gene="csaB"
                     /locus_tag="PLE39_13265"
     CDS             complement(14308..17082)
                     /gene="csaB"
                     /locus_tag="PLE39_13265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HNE69347.1"
                     /translation="MDKNNKKTILVAGYYGFGNVGDEAILSAILTDLRKQREGLEFIV
                     ISSNPEETAASHNVRSVHWKEINALMDAARECDLIILGGGGIFQDYWGTPAGTHLTEF
                     HWGISYYSSIGLLASLFDKPFMIYSVGIGPLLTDEGKRWTRLAFELADAATVRDVESR
                     DLLASLGFPKKQILIKPDPALNLSPKLKAAAEILQAEGISPKERPMVGVCIRNWTEGE
                     KGGAWKQELASALDAFLERHNAQIVFIPFQVGEHELENDYTVSVEVISMMQNQERTKV
                     LPQAYSPEVVEGLIARCRVVIGMRLHSLVFAASVGVPSVALIYDPKVRNLMKFLGLSE
                     YALDLQTMNVDSLSDTFHYLWMDQKQVRASLAEQGQKFKQLVQTNTTLALKLLDQKTK
                     RIEKPVALQLIRDLAVKQTGAFFVREKEAQALTAEVASLNQQVADQLARIAINEYEID
                     RIQKIVDYQQYQLHEMVHSTSWKIALIFQKIRTTLIPLGSRREKLVRFVFHWFVRKPL
                     SLISRIRTSIRVNGVFGAVLKGFVAIWVALTYPIKKAIFKQRYERELKELEAIIAGHN
                     GFFDLFHIPMGWNTMLFQRFQHVSLQSAKLGGIAIYGGHPTVDKDIFVYKKAAENLYV
                     FKATDPQITERILQALEKKGNSTVLRIESIDLVTTAEDVANFLRRGFRVVYEYIDEIT
                     SDITGNVPELVRDRHQALLRNEAVFVVATSDQLYEEVARHRAKNFVLSTNGVDLENWR
                     IPKGEPPVDMKSAFTGNTIVAYHGALAKWLDYDLLRMIADEGSYELVLIGHEHDDAFE
                     KSGLKGHPRVHFLGSKPYSQLNQYAIHYGVTILPFKKSNLTEAVSPVKIFEYMAALKP
                     IVTTDLRECKKYKSCLTAETNEEFMQQLKRASELRSDEEYLKVLDTEANENSWEHKTI
                     EILHMTGVKL"
BASE COUNT         4247 a         4229 c         4669 g         4034 t
ORIGIN      
        1 catcttcttc agaggggtct gcaccatatg ccatggcgag ataatacgcc atgtagtcac
       61 caaagatcat caaggtccat aaatgagcaa tgaccgagtc gccgcgggct tcgatcacat
      121 cggtgttcaa ggcttcgagc atgaaggctt gacgcatcaa atcagaacgc agtttgttgc
      181 gcggatggtc gctgggcgct ttcatgaaaa gggtcatggt gtgagcgttc agtgtgggat
      241 tcgggttgat ggtggcaatg agagtgttgt gcgtggattc agggatgatc tcaaaattgg
      301 cgccagcctt ggccaattgg ttgatctgca tcttccagcg gcgggccacg ggggcgagat
      361 tttccgtgcc gacgaaggtg acccagcgcc ccaccaattg acccgcgtac cgtttggctg
      421 gattctttgc agcgatcaca tcagcggtga tgtgttgctg agagcgtttc atcatggcga
      481 cggcttcagc gacatcgctg gcagggtcag ggatcaaccc aagacgggcg aacaaggcga
      541 gtaacaggcc aaaagggtag gcaacagcgg ctgtatccac cacgccgttg gaatcgaatt
      601 tccaggcagg tatattttta tcagcagcac gcatggcgat ctcgccgcct gtggagatga
      661 ccatcactgt gcagttgttt ttttgcgcgg cttcgaaaac atcgagcact tcttcggaat
      721 tgcccgaatg cgaaatgcag acaacgagag tctgaggtcc gctggcgaag gcggggaggc
      781 cgtagccgcg atgcaatgcc acggggaggc gaatgcttga ggaaacagag gcaattacca
      841 gttcggcagc gaccgctgag tcacccatcg cagaaatgac gatcgtttga atatccttga
      901 aagcaggcaa cggcagggtt tgtcccagtt cccatgcggt ttgcaattga ttcggcaggc
      961 cgtcaatttg ggcgagcatg ttgcgggtat ctattttttt gaagaggtcg aggtcatcta
     1021 ggttcatgga attaggtggt ggaaaagttt tatgaaatca ggcggcagac cgcggtcaat
     1081 tgccatcggt ctgccgcctt ctgtctgcgg tcgttagctc gcgtccttga tgaatttgct
     1141 caagtctttc ttcaactgct tgagcgaagg gatgtggacg tacatcatgt ggcctgcttc
     1201 gtaatacccc atgctgatgt tcttgcgcag ggattcgtcg aggccgagat gatcgaaggt
     1261 gtattcggtg gcaaagtagg gtgtgccgag gtcgtaatag ccgttggcga cgaagacctt
     1321 gaggtattta ttaaaggtca ttgaagcgcg cagggtttcg gcaacattga cgtattgatt
     1381 ttgaaaatat tcatacgacc aattgaggaa gactttgtcg ctgaggatct cgtaaggcag
     1441 gtcggtttcg aatttgagct cggtgcgcag gtaatcatta agcgtggccg tgtaagggcc
     1501 attgacctgg gcgaacagcg ggtcataatc gggcatggcg gtgacgccat tgcggtctat
     1561 acctgtgaag cggctgtcca agcggccgac ggttttccca cgctcgcgca ggagttcctt
     1621 gaagaaatgc tggtcgtaga tgcgcaggtt ggtgcgttca ataaactcct ttgaaatgcc
     1681 tgtgtaacgg gataattttt cgacgacact ggcgcgttct tcaggagtga gagccgcacc
     1741 tttgagcagc gcggtggcat attcgccttt tgcaaattct tcagcttcgc gaacgacttc
     1801 cttcaacgac gctttgaagc tcaacttgcc gtgataccat gccgtagccg cataagcggg
     1861 gacaaataaa atatacggca ggtcgttatt gaggttgaaa tcaatggtgg tgaaatcaag
     1921 cactgcagaa atgaggatga ggccattgag gaacatgcca tgacgttcct gcaaatagcc
     1981 tgaaagaccc gaggcgcggg tggtgccata agattcgcct gcgagaaatt tcggcgagag
     2041 ccagcggttg taacgcgtgg aatacagtcg gatgaaatca ccgaccgagg caatatcttt
     2101 cgagaaggtg tgccactcgc gtgatttctc accttcaaca gggcggctgt agccagtgct
     2161 catcgggtca atgaaaacga ggtcggtttc atcgaggatg gaaaaagcat tatcggtgag
     2221 tttgaatggc ggcgggggca tttcgccttc gtcggtgagg acgacgcgac gcggtcccaa
     2281 cacacccatg tgcagccaca cagacgagga gcctggtccg ccgttgaagg aaaaggtgat
     2341 cgggcgtttg gatctgtctt tgacgccatc gagtgtgtag gccacgaaga acatttgggc
     2401 gcggggctta tccacctcgg cctctttgtc cttatcccac acttcttctt tgacgaccat
     2461 cgttcccgtg gtgacggtgt atttgatcgt cttcccgccg atttttaccg agtgtttggt
     2521 ttccaccagg ttgtcttttg gagcgggttt ctcgtccttc ttttcatctt tcttttcttc
     2581 aggtttcttt tcagccattt ttatctccca tgaaaatagg ttgattgggg tcactggaat
     2641 tactgttgcg tggagcaaat atagcataaa gaagggcgac ttccattaca actcgaaacg
     2701 gaacacagat aaaggtcaac gtggtgaaga tgctgccgat atcctgtgta gaaccgccct
     2761 gatccaccat gtacatgggc aaaacaacat acacttgcgt ggccagtccg atcaaaagag
     2821 ccagcccgaa gtaaatgccc gcggcaagcg ccgtgcgcgg atattccttc cgtcgtgaaa
     2881 tggagaatcc aagcccgacg gcgtggagga acagggacgg aagctggggg agaatgctgg
     2941 agaagatagc ggtaaggtat tccatttttt atcctatatg agaattttca ccacgagcac
     3001 atataaacag ggtggcttca atcatcagca gcgcaataaa gtactgctga aatctacaac
     3061 tagcggacgt atattttgga ggcaagatca tcccaattcg ggacgataag ctccaatacg
     3121 gctacagccg tccattcaaa tggtttcaga gaaaggtagg cggcatccgc aaggagggga
     3181 ttctgaattc ttcgggcaag cgcctttccg aaaagatcat aaatggagcg gagaattttg
     3241 tgcagatgcg gcacgataac catcagggtg agttcggcac atttcagatt ttgcaattgc
     3301 ctgttcacgc gcatggatat tccattctcc aggcggatct cttgtgaacc gacaaaagca
     3361 gaatgtccat ttgcggcggc ggtggcaatg taacaattgg gcggctgggt cggcagggtg
     3421 gcatatagtt cgtacatctt cgtaatgttg tagtacaggg caaccccata cgcagaaagc
     3481 caggtaaaaa gcccaaaacc atgcgcatgg gtgagtttgg gttcatgata tttccataac
     3541 caacgggcgg cttgcaaagc gatgaagaaa caccaaaagg gtgcagacat cggcaccaag
     3601 atgaccagaa gaaggacagg ggatgtgctg taatcaaaaa ccgcggcgac aatgccccac
     3661 aaaaccagaa tcgcaaatac ggcaaaaatc aatgcacgaa aaaccttttc gttctgaagc
     3721 catgcacgaa gttttggaat aagaagcggg agaaatataa aaaaaacaaa gccaaataaa
     3781 acaggcgaca agtccatgga cagggctgtg aggaggctat agtgaaagga aaggagcact
     3841 ccgccataaa tgccaaatcg aataacgagg gattcgctct ggcgttcttc gtcactgatc
     3901 agcatcaggt aggagataac cgaataccca agcaggggca gggtcaatgc ggctgcttcg
     3961 ggggaaagca tcaacagcat gtaatcggaa aacttccctg tctgccataa aggcagcatg
     4021 gccgaaacaa acgaaaagtt gactattgga gcaactggga taaagatcca atacagcaac
     4081 cgttgagaaa aggagccaag cagtggtggg atggtggggc tgttctgttc gttcatttca
     4141 ccaaattgac caacgcatac acgtcttttt tattccaacc gctttgcccg gccaggtctg
     4201 cagaaatttc ctttgcggac cttcccgttt gcaattcttt ccgaatggca tccatcaact
     4261 cagattcttc ccacttgctg ctttcttcct tcttcttccc ttcgaccacc aacgtaaatt
     4321 cgccccgagg gtctttggat ttgaaatatt ccagcgcacc gctgatatct ccacgccagt
     4381 attcttcgta gcgcttggtc atttcacggg caatgcagat gcggcgatcg ccgaggacgg
     4441 cgagaatgtc ttcaagtgag tcaagcaagc ggtgtggggt ttcgaggaag atcaacgtgt
     4501 agggcaggtt actaacttgc cccacaaaac caagccgttc actcgattta tggggaagat
     4561 agccaaggta taaaaatgag tctgtgggca ggccggaaac ggtcaaagcg gcgatggggg
     4621 ccgagggacc aggaacaggc acaacgtcga agttggaagc gagggcagcc tgtaccagtt
     4681 cgtagccggg gtcattgatg gcgggagtcc ctgcgtcgga gatcaaggcc acatcccctt
     4741 ctgcaagggc ggagaggatc tgatccaatt tatgaatttt attgtgttca aaatagctgg
     4801 tgagtctggt ttcgatcccg aagtgtttga gcaacacacc gctgtgacgg gtatcttccg
     4861 ctgcgatgag ttggacttct ttgaggatgc gcagggcgcg cggggaaata tcttcgaggt
     4921 ttccgatcgg ggttgctaca agaaaaaggg tgcccatttg tttattttat cattctgttc
     4981 cggtaattcg ttttgccgca ggtaaatcag atatgagggt gaatcaaaac gcggattctt
     5041 tttgaatccg cgttcatcta tgcttgtcgg cgagttttta tatcggggaa ctattttact
     5101 gacaggagcg aaggtcgatc ttaaaggtgg tgcagcccgc gccatctttc ggggtgggca
     5161 cacagcattg attggctgcc tcaaagagcg aacccgattc acatttattg aggtcttcat
     5221 tcgcgaccac aggcggcgta caaactttga gttcgtagga ataaagctgc ctgcccgtac
     5281 atgcgacgat gctctttcca tcggatgtgg tttcgatggc acaggtcata tcggggctga
     5341 gtgattcgaa ttccgcaccc tctgggatgg aaaccgaggt gtagggaatc ttggtgacac
     5401 acgccttgtt cgtctcgcgc acttcaggaa tggggatggt aatcccctcc gcacaggctg
     5461 gagcaaaagg cagaggcgaa gcgaccgttt cggtcggcgg ctcggaggtc agggtcggtt
     5521 tttcggtggg aacttcttcc accgtggaag tgaaaacaac ggcggtcaat gtaggtgtgg
     5581 aagtggcttc cggctgggtg aataattgat tgcgcaacag gaatccccca accacaacca
     5641 ccagcaaaac cacaccagcc acggcaaggc ctgtccgcgc gcggggcgat tgcagggtgg
     5701 atgtacccgg aagaacggtt cgaaacccgg tgttcgaaag acctgccttg ccttcatttc
     5761 caaatgcaac catattcatg gccttggcca gttccactgc attggcgtaa cggcgggtct
     5821 tgtctttggc catacacatc ttgatgatgc catccacttc aatgggcagg ctgggaatca
     5881 tattgaggat ttcaggcacg ggatcggtga tatgttttac caccacaccc atgggagtat
     5941 cggcgttgta tggctgttga ccgctcaaca tttgataaat gatgacgccc aacccgtaaa
     6001 tatcgctgcg cccatcgatc ggctcccctt ttgcctgttc cggactcatg taagcgggtg
     6061 taccgatcac gccactgcct gtcaggctgc cgctggaaga ttcggtcaac tttgcaattc
     6121 cgaaatcaga aatgaacggg tcaccgttgt tatcgaagag gatgttatcg ggtttcaggt
     6181 cgcgatggac aatgcccttt ttgtgggcat agtgaagccc cattgcaatc cgttcaacaa
     6241 tgcgggccgt atcttgtaca gagatcttcc ccaatgcgat ctggtctgaa agcgagccgc
     6301 ccgtcatgta acgcatgacg aagtaaggct ggccgtcttc ctcgcccaca tcgtacaccg
     6361 gcacaatgga gggatgctcc aaaccggcaa cgatcttcaa ctcccgatca aaacgcccac
     6421 gaaactgtgg atcgtgcaac atttcgcgtg gcaggacttt gattgcgact tcgcggtcga
     6481 agctgggatc atatgcgcgg tacacagtag ccatgccacc gcgccccaac tctgatttga
     6541 cctcatatcg tccgattttc gatgggatag gcataagccc aagtataacc acatcaatat
     6601 gatgatgcaa gcaatgatgc agtttttaga caaatgcacc cgcagatgac acgcccctca
     6661 aaaatcgtca cctgcggatg caaacccgct tctctaatcc gctgcggcct gcgcggtgtg
     6721 aatggatacg gtccgcgtct gggtcatggc acgatacccc gcccaaatca aagagatccc
     6781 gatgaattcg gtcacgaaga gcacctccac gtacccaaaa cgggtgaagg aaccgccgat
     6841 gcccggcaaa agtgcgccaa tggcaatgag ccagttgcca agcacgcggc tgccagaatc
     6901 caatttgcgg gcatactgaa ttgccgacca ggccgcacct cccacaagaa agatcatggc
     6961 ataagtattg ggaagaatgg agaacatacg agcccaggtc caggtcatta ctttgccggt
     7021 caggcgggct gggtcaacca gagacatatc caccggggcg agaaagatcg ccaccgaagc
     7081 gactgcaata taaaccacga agaaccaggc caggaaatct gcggtctttt tcttcaacaa
     7141 cagatacacc gtcccctgcg caagcgggaa tgcaccgagc aacgcgccgc tgatatacca
     7201 ggctttcatg ttccatacac tccaaccaac cagcgtatta atggactcgg tcaacgtgcc
     7261 gatgccaaaa caaaacgctc caatggtcca ccacatgagg tatctggctt ccggcttgcg
     7321 ctgccagtgt ttatacaaag caaccgtgaa agagaatgag atcacggtgg taaagatggg
     7381 aatatagtgg gtaacagctt gcatttgaaa ctcctttagg ttttatatca tcgcgaaggg
     7441 ctgcaccgtt ccgcaacgat gggtgaacaa ataaaaatgc caaccgttca acggctggca
     7501 ttcaatgact gataagtttg caaaaggctg atcaaaattt catctgtaaa cacatgcatc
     7561 ttttggaaca tttctgcagt ttgtcccgaa ggcacattgg cttcgccgta caccttgatc
     7621 accgattcga tcagcgtatt tctgaaaaac aaaaaagcct tggctgcatc cacgtaattc
     7681 aaactgtaac gatgggctcg tgaagcatat tcataaccga tggcataaga ttcgctttca
     7741 gcctctgccc cgtgggttgc gacataattc atcaagccat ggaacagcga ccttgcactc
     7801 atgcggtact gcgtccgcgc atcgtcatcc agtttttgat accaggattc agcctgcaag
     7861 cgtccctctg aaatctgcag gcggacattt ttgacgactt cattcatcat cccttcgggt
     7921 tcgatcgcat tcgacctgtg atttgattct gcccaaaggg aaatttcact gcgccgataa
     7981 cgacggtgcc cgccttgtgt tttatgcgta ggcaggagac ctttatcaga ccataacctg
     8041 accgtgccag gatgcacccc caggatcttt gccgcatcac tcagggaaag ccattcgtcg
     8101 gtcataatta ctccacgctc agcgccactt tttccacata tgacggttca tgggcctgct
     8161 tttggaatgc gcccacatca atacgataca acatgaccgc ggcagccagc agcatcaatg
     8221 cttcaaggct gaagacaacg aggtatccgc tcagggcact gcctgtcatc tgtgtggcca
     8281 ggtctgcaac cacacccgcc atcaacaacc caaccaggcg ggaaagcccg ttcgaaaaac
     8341 cccatgcgcc aatatacaac ccaacctttt cagggacggt caggtcgaac atcaacgaca
     8401 ggttcgcaac tgtggaaata cccgtgccga atcccagcag ggtgatccca aggtaaaaag
     8461 ccgtcaggct gcctgtcaac ccgctgacga cgatgatgag aaaaccgatg agcgcgccaa
     8521 ggttgcccat ttgggcaatg tatttcttct tcacccgccc ttccagaaca ccagccacaa
     8581 tgaatgccac cagtgtaaaa gtgctgctga tggatacgat gcgtgaggtt tgttcgaggc
     8641 tcatgccgaa tgcctgcgcc ccaaagggct cgagcagaac atcctgaccg aggatggcgg
     8701 caagcaaaag caacagatag gcaaaaaata tctttgccac agggttggag gtgatggcag
     8761 cggtcatctc tttgacggtg tatgtttcag agcgtggggt ttggtccgat ctgccaaagg
     8821 gcggctcaag cttgaagagg ccgagcaggc caagggtcag ggcagaagcg gcgacgatga
     8881 gaaaggcacg cgaaagcgtt tcagggtcat aaactgtcac catgcggctc aggctgatcc
     8941 ccgtcgcaat taaaccgatc accatgatgg tgaacatcgt tgcgacggtt tttccacgcc
     9001 ccttttctcc tgaaagttcg gaggcgagcg aaaggtagga aacagcagag aggttgtagc
     9061 ccatgcccca caacccaaat gcgaagatgc ccaccatgat cccaagtgtg aagttttcac
     9121 tgaccaacac ggcgatctgc ggagcaatgg ccacacccaa cacgcaaagg agtaaccccg
     9181 caaggatgta tggagtacgg cgatatccca tgatggggtg tcggtctgag aacgagccga
     9241 tggccacctg aatgggcgaa agtaaataag ggaaaatggc caaaagtgtg aacagggttt
     9301 tggaaacgcc cagatcgaag atcatcacac ggttgagtgt gctgttgatc ggcacaaggg
     9361 tgatggcaac ggcggtatgg atcagggcta attgaagtcg tttgatgaac atagtgggtg
     9421 gttttgtctc ttggtcattg gttttgttaa acaaagcaag ggcgttttac gcgccctcac
     9481 ctcattgtgt attttaggcg taaatcttca tagagccatg tagatttatg tagaatttta
     9541 ctagagttta gtgagggttt ggggcatgca ttagctggct tactttttcc cgaattgcct
     9601 gatcagcgca tcgtatcttt ccaccagtgc gggcgcaggg actttgccgc caaaattgag
     9661 aaagactttg aatgcggcgg caccttgccg atcgaagaat tgaaagctga gcgtctcgat
     9721 cccatcaaga tggctgggct tgcggaaggc aaagaccgaa tccaattccc aactgcggat
     9781 gtgcatgtcc agcgatttgg tgcggacgtt caaaaagccg ccagtatttg tgagattgcc
     9841 aaactgtcca taggcttcga tggttgtggc tccatttgag acgatgacat gcacattgcc
     9901 cagcgtttca aaagcgcaga cgatctcctg ccagcgtgag aaatccagtt catgtgcaga
     9961 gtcaccctcc aatgcgcgaa caatttccac ttcagggatt cggagttgat gggccagcat
    10021 taatgtcatc tgcgaacggt cagtgttata agcctcgcgg atggttttct ggcgttcgac
    10081 aacggtcaga tccaggttga ggggttgaat ggtttgaaac atactacatc ctttgataaa
    10141 taaattggat tactttcatc ctgttcaagc gacgcgtagt ataatttcgg ggatttgaat
    10201 aatctagata tttgtattaa attttatcta aatagagttg atttggattt tcaatcgaat
    10261 tgaagatttt ggagttgttt acaaggaatg tcagccagtt ctgctatgca acgctatgcc
    10321 gcggagatct atcgcctgca acaagatcat gagcaggtgc cgctttcgct tttaagcacg
    10381 catgttgaat catcagcgca ggccattgcc agcatggtca aacgtttgca aaaaaacggg
    10441 tatcttgtcc atgagccgta tcgcggtgta cgcctgaccc ccactggcga aaagatcgcc
    10501 atgccttccc tgcgccgcca tcgcctaacg gaggtcttcc tcgtcaaggt gatgaaatac
    10561 gattgggcca ctgcccatca actggctgat actttcgaac tcggcgtcaa tgacatgatc
    10621 gaagaccgta tggatgaact ggctggccgc cctacacgct gcccgcacgg agagccgatt
    10681 cccacaaaag acggggtgat gcctgtggtc cgggacatgt cactgatcga tgtgccctca
    10741 gggtcagatt gtgtcatcag ccgtgtgcgg acccatgatt tcgaaaaact gacctacatc
    10801 ggtgagttgg gtcttgtgcc cggcacgtcg tttcacttgt taagttgtgc cccgttcaag
    10861 ggtccgttgc ggttgaacat gaagccgcac gaccatgtcg tcggttatga attggcggca
    10921 tcgctttggg ttgaagttac gaaggaaggc gaaggcaaca aattgccagc gcaaaagaac
    10981 aattgacccc tgaattttcc tgtggctttt aaagcacgtt aagaaaaact gtccgctccg
    11041 tggagtggac agtttttgat ttacggtttg ggtgtgctga cgatcttctt tctgcggaac
    11101 aggtttgtga acagggcata gccgagcttg gtcagcatca tcaaataata caaatagttg
    11161 aacaggaaga agtaatcctt cgcgaagtgc ttgcggaaat aaagataata cgaacgatgc
    11221 cattcgatga tggtgcggta tggctggacc tttgagccgc cctcccctcc gtagtgcgtg
    11281 atctgcgccg tgggcacata aaaaatcttc caacctgcca gacgggtgcg gcgacaataa
    11341 tcggaatcct cctggtaggc gaagaaatct tcgtcgaaat atccgacctg ctccacgact
    11401 tcacgacgaa cgaccatgca cgaacctgaa actgcctctg cttcgtgagt gaggtcctcg
    11461 ggcaggtagg tcatcagata tcctgcaaaa cgtttgtctt gggggaaaag acgggaaagg
    11521 cccgagaaat aacagatcgc atcccatgga cgagcttcac tgcggcggca ttgcttttga
    11581 agcgtgccgt cacggttcaa cactttgggg ctgacaatgc ccacctcggg atgtgaatcc
    11641 aggaagccaa caagttcggc aaaggctttc ggaaagatga cagtatcggg gttgaggacg
    11701 acaacatggc gaccttgtgc cagccgcatg gcctggttgt agggacgggt aaagccagtg
    11761 ttgccttcgt tggtcagcaa tttcacatcg gggaattcac tgcggatcat ttcagggccg
    11821 ccatcgccgg agaaattgtc aacgacgatg atctcaaagg tgagcgtatc cgttgtcccg
    11881 tagatggacc gcagacagtc gcgcaggaaa tccttcgcgt tgtaattcgt aatgcagatc
    11941 gaaatatcag gtttcatgaa ttttccgtgt tgcgttttag aaaagtgagg aagggagcca
    12001 gccaattgcg cgacatggca cgatgcaatt cagcgatgga tgcttttcta tttctctgga
    12061 tcgcgccgcg ttttttgagc atgggggcta atccgcgcag ggcatcgaat tttgcgcgcc
    12121 agatggtacg gccctgccct ttggcggtga agtaaaacaa gaagaacaca ttgatgaaaa
    12181 tatgcagcgg caggtagaac caaaacagca cagacggcat gtctttggcg taggtccaca
    12241 ccaggttacg gtggccgtga taaatggaaa agtcgctttt cttgccgaag gtgaccgagc
    12301 cgacatgatg cacaaccgcg cggggaacta caaaacactt caagccgtgt aatctcagac
    12361 gaaaaccgag gtcaacatct tcgtgatagg aaaggtaatc ttcgtcgaaa ccgcccacgc
    12421 tgagaaattt ttcgcgcgga aaaaatgcgg cggctccgca ggcgctgaag acttcgcggg
    12481 tttcatccag gggataggtg cgtgagccat aaccgtttct ccacgccaac ccgctgacgt
    12541 gatacacgtc accttcacca tccagcaatt cgggtgtatt ggcctgaagt tggcgcgagg
    12601 aaaaaaatgc gttcgggtgc atctgggccg cttcgagcaa acgctccagc caatcgggtt
    12661 cggggaaggc atctgtgttg agcagggtca gccatttgcc agtggcaagc tgtgcgccga
    12721 ggttgtttgc aaatgaaaag ccgtgatttg tttcgagccg ttccacgcgc agggcaaggg
    12781 tgggatattt ttcctgcagg cccacacacc catcatcctt tgagccattg tccacaacaa
    12841 tgatctcaaa atcctgcacg gtttgcgcaa gcagatgctg gaaacagcgc ggcatataag
    12901 cagcgctgtt ccaggcaaca agcacgatgg agatgagggg ggcattcatg ttgaaataag
    12961 ccacgaagaa ttacaaagag acgcgaaggg atatctattc gccttcggct ttgcgggtct
    13021 cttcaatggc ttcgcgggtg gcacgcagga aggcgtaacc gtatttgcgg tcgggttcga
    13081 ggtaggcaca ttcgccccat tcattccagg cattgacgaa gatgaagggc gcatcaagtt
    13141 ccttgctgct gcgggcttca cgcagaatgt caatgagcca ttccttgtac aaggcggggc
    13201 tggcgccttc gaagatcatg ggggtgttat tgcggcgggg cgtgttatcc cacatgggca
    13261 taatggcacg gtgtaatttc ttcgcctgaa acttgaaata ttgcttgttc ttcaccaggt
    13321 ccacgtaatc gaggacgatt ccgccgaaat catcgcgcat gaagttgatt tcgttggtga
    13381 tgtttttaca atagcggaag accgtgcccg ggtggaattc gctttgggcg tcaaaaccaa
    13441 gcgccaacag gtcgccatca aaggtgtttt ccttgaccgc aatgatgtat gggttgccaa
    13501 gtccttcctt ttcgcaatat gcccgccagt gcgcaagtac attggaacaa tcaggcacga
    13561 aagatgggcg gtacacgatc agcaccgggc ggccatccac acggatgtaa cgcttgtcgg
    13621 cggcataggg cttgatgctt tcgatgaagg atttataact ctccaccgat tcgttttgat
    13681 acatgatgac ctcgccgctg gacccgtcga agcggcgggt ccacgattcg tttgcccagc
    13741 acaggcagaa ggggaaatcg agatcggggt tattcaaaaa catatcaaga ggtttatcaa
    13801 gcaaacgacg gccatcgaac cagtaaaagt aataggcgaa gccgtgaatg ccgtgcgatt
    13861 tcgccaattc gatctggcgg gcgatgtttt ccttgagacg caggtcatac gatcccaact
    13921 caccaggcag gcgcggctgg taatgcccca cgtactgcgg cacagccttg gccacgttgt
    13981 gccactcggt gatgccgcgt ccccaccact cgtcattttc aggggttggg tgaaactgcg
    14041 gcaggtaata tgcaagcaaa agcggatcgc ccgcctgcgg cacataggca tcggaagcca
    14101 gatccacata gtcatcggtc ttatcgggaa ttgccagaac ctgtttggtg tactgttcgt
    14161 acattttcgt cttcgcaggc tcgggcatgg caggcgcact gcgcggacgg aagatccgct
    14221 gtgacagcat cgaaagcctg cggcgaagcg gctcaggcat tggcaatttg cgataaatac
    14281 gcagcgcgaa ttgaagtatt cgattgatca cagtttcacc ccggtcatgt gcaggatctc
    14341 gatggtcttg tgttcccacg aattctcgtt cgcttccgta tcgagcacct tgagatattc
    14401 ttcatcgctc cgcagttcag aggcacgttt caattgctgc atgaattcct cattcgtttc
    14461 agccgtaagg caggacttgt atttcttgca ctcgcgcagg tcggtggtga cgatcggttt
    14521 caaggctgcc atgtattcga atatcttcac aggcgagacc gcctcggtga gattggattt
    14581 cttgaacggc aggatcgtca caccgtaatg gatcgcatat tgatttaact gtgagtaggg
    14641 tttgcttccg aggaaatgca cccgcggatg ccccttcaaa ccgcttttct cgaaggcatc
    14701 gtcatgctca tggccgatca gcaccagttc ataactgcct tcatcggcga tcatgcgcag
    14761 caggtcgtaa tcgagccatt tggcaagcgc gccatgatag gcaacgatgg tatttccagt
    14821 aaacgcagat ttcatatcca cgggcggctc gcctttggga atgcgccagt tttcgaggtc
    14881 tacgccgttg gtgctgagga caaaattctt tgcgcggtga cgggcaactt cttcatacag
    14941 ttgatcggag gttgccacaa caaacacggc ttcatttctc aacaaggctt gatgccgatc
    15001 acggacaagt tcgggcacgt tgccggtgat gtctgatgtg atctcgtcaa tgtattcata
    15061 taccacacgg aaaccgcggc gcaggaagtt tgccacatct tcggcggtcg tcaccaggtc
    15121 aatggattcg atgcgtagca cggtggaatt gccttttttc tcaagggctt gcaaaatgcg
    15181 ttccgtgatc tgcggatcgg tggctttgaa aacgtaaaga ttctcggcag cctttttata
    15241 aacaaagata tccttgtcca cagtggggtg gccgccatag atggcaattc cgcccaattt
    15301 ggcagattgc agcgaaacat gctggaagcg ctggaataac atcgtgttcc aacccatcgg
    15361 aatatggaac aggtcgaaga aaccgttatg tccggcgatg atggcttcca actctttcaa
    15421 ctcgcgttca tagcgttgct tgaagatggc cttcttgatc gggtaggtca gcgcaaccca
    15481 aatggcgaca aagcccttaa ggacggcacc aaacaccccg ttgactcgga tcgaggtccg
    15541 tatccgcgag atcaaagaca atggtttgcg aacgaaccaa tggaaaacaa agcgtaccaa
    15601 cttctcgcgt cggctgccaa gcggaatgag ggtggttctg atcttttgga aaatcagcgc
    15661 gatcttccat gaagtgctgt ggaccatttc atgcagttga tactgctggt agtccacgat
    15721 cttttggatg cggtcgatct cgtattcgtt gatggcgatg cgtgcaagtt ggtcggccac
    15781 ttgctggtta aggctcgcta cttcggcagt caaagcctgc gcctcttttt cacggacgaa
    15841 gaaggcgccc gtctgtttaa cagccagatc gcggatcaat tgcaacgcca cgggcttttc
    15901 aatgcgcttg gtcttttggt cgagcaattt caaagccaga gtggtgttgg tttgaacaag
    15961 ctgcttaaac ttctgaccct gctccgccag tgatgcgcgc acctgttttt ggtccatcca
    16021 aaggtagtgg aaggtatctg aaagcgaatc cacattcatg gtctgaagat cgagcgcgta
    16081 ctctgaaaga ccgaggaact tcatcaagtt cctgaccttc ggatcgtaga tcaatgcaac
    16141 cgatggaacg ccgacactgg ccgcaaaaac aagcgaatgc agacgcatgc cgatgacaac
    16201 gcggcagcgg gcgataagcc cttcgaccac ttccggggaa taggcttgcg gtaaaacctt
    16261 cgtccgttcc tgattctgca tcatggagat gacctccact gaaacagtgt agtcattctc
    16321 gagttcgtgc tcacccacct ggaagggaat gaacacaatc tgcgcattat gtctttcgag
    16381 gaaggcgtcc agtgcggacg ccagttcctg cttccatgcg ccgccttttt cgccttcggt
    16441 ccaattgcgg atacaaacgc cgaccatggg gcgttctttg ggagaaatgc cctcagcttg
    16501 aagaatctcc gccgcggctt tcagtttcgg ggaaaggttg agcgcagggt cgggtttgat
    16561 gagaatttgt tttttgggaa aacccagtga agccagcaga tcccttgatt cgacatcgcg
    16621 taccgtggcg gcatcggcca gttcaaaggc gaggcgcgtc cagcgtttgc cttcatcggt
    16681 caacagcgga ccaatgccca cggaatagat catgaagggt ttatcgaaca gggaagccag
    16741 caagccgatg ctgctgtaat acgaaatgcc ccagtgaaat tcggttaaat gcgtgcctgc
    16801 aggggtgccc caataatcct gaaaaatgcc gccaccgcca aggatgatga ggtcgcattc
    16861 gcgggccgca tccatcaagg cgttgatctc cttccaatgc accgaacgga cattgtgcga
    16921 tgcggcggtt tcttcggggt tcgacgagat gacgatgaat tcaagtccct cgcgttgttt
    16981 tctcaagtcg gtcaggatgg cgctgaggat ggcttcgtca ccgacgttgc caaacccgta
    17041 ataccctgcc accagaatgg tctttttatt gtttttatcc actgtctata ctgcctctcc
    17101 aggaaatgtc cgcctattat catcgaactc catgcaggag gcaatgatga taaattgcca
    17161 gaaaaaagta gcgcgacat
//
LOCUS       DAOQJC010000089        17071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1591_length_17071_cov_9.434121, whole genome shotgun sequence.
ACCESSION   DAOQJC010000089 DAOQJC010000000
VERSION     DAOQJC010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 17071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17071
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1591_length_17071_cov_9.434121"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLE39_13270"
     CDS             complement(<1..211)
                     /locus_tag="PLE39_13270"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE69348.1"
                     /translation="MAKVFISYSRKDIDFAKKLTGELQKSELDFWVDWEGIPPTVDWW
                     REVEKGIEEADVFLFLISPDSVASKV"
     gene            complement(291..2117)
                     /locus_tag="PLE39_13275"
     CDS             complement(291..2117)
                     /locus_tag="PLE39_13275"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNE69349.1"
                     /translation="MYGWHLKILRVNLTTHKVTTEDVDPKIARDYLGGRGWAIHYLYK
                     EMDPMADPLSPENKLIFATGPLTATPAPTGNRYMVVTKSPLTGALAHSNSGGEFPTWM
                     KRTGFDLYIFEGRSPEPVYLFVNEDQVEVRSAAHVWGKDTHETTDILKAETSEEARVA
                     CIGPAGENLALMAAIMNDKHRAAARSGVGAVMGSKNLKAVVAMGSKNPPLHDPEGMRT
                     LSVETSKNVGADVKKGSNMRIYGTSYVPQVTNTLGILPTHNFLQGTFEHVHNVDGDAL
                     KNQYLIRHTPCYRCPLSCGRLTEVPDGPYKGKGEGPEYETISSLGTGCGVSNLAALVK
                     ANYLCNEYGMDTISTGMTIAAAMEMYEKGYIPEEVIGQPLKFGDHDAMIAMIKLMAYN
                     EGFGKELAQGSYRLAEKYGHPEIAVTTRKQEFPGYDPRGSQGMGLLYATSNKGASHME
                     GDVAYEEVFGVPVKENPLTTEGKPELVKHFEDSFALMDSSGVCVFVGIRYMFNKDRMI
                     LPQTLANMMNLSTGAGYTAEEALRAADRVYTLERMFLLKAGSTEDTLPHRMLHEPLPD
                     GPAKGKVVELDKMLPEFYKLRGWDEKGWPTKEKLADLGLPLN"
     gene            complement(2214..2453)
                     /locus_tag="PLE39_13280"
     CDS             complement(2214..2453)
                     /locus_tag="PLE39_13280"
                     /inference="COORDINATES: protein motif:HMM:NF025807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNE69350.1"
                     /translation="MMIRVKLIANYRAALPPEAEHGVVELNVPDGTTVYDAISRFDIP
                     LNDESVIVLNGLTVDMNTPLQEGDMVTAFSAIAGG"
     gene            2492..2932
                     /locus_tag="PLE39_13285"
     CDS             2492..2932
                     /locus_tag="PLE39_13285"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNE69351.1"
                     /translation="MKRQIPYHGWCFVCGNENPRGIGITMFVDDNGILTSEFTLNETH
                     QGPPGYSHGGASAAILDEVMGLVVWAAGHKVVAANININYRKPLPLHQPLLAEARISE
                     VGERKITSVGEIRLADSTVAVSGSGVYVVAPHLFEAKRLKGVVS"
     gene            2929..3627
                     /locus_tag="PLE39_13290"
     CDS             2929..3627
                     /locus_tag="PLE39_13290"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNE69352.1"
                     /translation="MKTIGLIGGMSWESSIEYYRIINETAKEKLGGLHSAKSLMVTVD
                     FAEIEKLQHEDRWDEAAQILVQCAQDLEHGGADFIVLCTNTMHKLADQITANVSIPFL
                     HIADATAEKIRAAGLKRIGLLGTRFTMEHDFYKGRLVQNFGLEVLIPEQTDRDIVHRV
                     IYDELVQGKIVETSREEYKRIIEWLIEQGAEGIILGCTEIELLVKQNDSRVPLFPTTL
                     IHAVAAVEKALQED"
     gene            3634..4089
                     /locus_tag="PLE39_13295"
     CDS             3634..4089
                     /locus_tag="PLE39_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69353.1"
                     /translation="MRIFHAIFFSLLLSACLTMPGPDTTPTADSPFVTVQEENPYSPK
                     PEDLGKQQASVIVTSVDLSERSDLSPVRVEVSIFGSMPSVCNELRVTVDPPTAAYEVH
                     IEAYSVADPNQNCENVFQQFEGHILLGVYTPGRYTIWVNDTFVGDMISN"
     gene            4165..4848
                     /locus_tag="PLE39_13300"
     CDS             4165..4848
                     /locus_tag="PLE39_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69354.1"
                     /translation="MFNFFKKKEKKPETSLADALRDVIFGVAPATEWPKGDTGEHPWS
                     VFIEARRHIMERQNFEEAAKLYKQITETPGLESRHYLQAWFFLRKMNIQPPADMARKA
                     YGIVVDVRLQSGPELVACYADHTARYLHSSGGGIVWEAPDTSLNDVIDALIRAGDEVA
                     SKVPPLENNIRPNAPQQVDAVQIGVLTPSGIHHGIGTFEGWSKDPMGSTIVNAASYLM
                     QNLVNKKKS"
     gene            complement(4936..6045)
                     /locus_tag="PLE39_13305"
     CDS             complement(4936..6045)
                     /locus_tag="PLE39_13305"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNE69355.1"
                     /translation="MIPGLDGLRAVAFLLVLGAHTDNLSFGWVGLQLFFVLSGFLITG
                     ILIRMKNSLGKREYFTKFYGRRFLRIVPLYYFYIFLMFGLKFAAPVLEFKPLLNEFGD
                     KFWLQVWPSIFYVYDFFHTTSAYEHSRFFTHLWSLSVEEQFYILWPLIVFLTPNEKMK
                     RLFFGAIALGPIMRGVVYWVYSYHRLPFMTTIPDIAVYAMPFSHIDAFAFGAFASQFE
                     IPRPRLQLFLASILVPAIGLSTQYLVEGRALWSTLGQGFLFSVGYMQIWGYSLLNYLF
                     MVLIYNVAKTGLFNHTLDLPPLRYLGKISYGLYVYHTAAIWLVLKLGSIDSGNMVLYG
                     ISFVVTAVVASLSYQVFEKPINDLKDKYFPLSAST"
     gene            complement(6089..7084)
                     /locus_tag="PLE39_13310"
     CDS             complement(6089..7084)
                     /locus_tag="PLE39_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69356.1"
                     /translation="MRKLSLIHLSILFLVAACNLPVATPVPTPHLFQTMQASTPVAVD
                     TAAPTAPVVEEPSEPTGKIAFTCQVFKELEREQICMVNADGTNYQRLTTEDATRHFYP
                     SISPDGLSVLYSALDEQSKHYEIYEFDLAAGEPKALTFALGDLNAPEVSPDGNSIAFT
                     RFFDDPEHPTTWLMDRDGGNPRQVSDVRAWDPTWSPDGTQILFASYADGLSQLFIINL
                     DGSGLRQVSHLPALRGRSDWSAAGLIVTYSGKPWERELFSMNPDGSDLQQISPAGGNS
                     QGPSFSPDGEWVAFTAYFDRFEVHGCEIYIMRVDGTDLRRLTDNDYCDYQPRWGP"
     gene            7233..9086
                     /gene="htpG"
                     /locus_tag="PLE39_13315"
     CDS             7233..9086
                     /gene="htpG"
                     /locus_tag="PLE39_13315"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNE69357.1"
                     /translation="MANDAPQQYTFKAETKQLLNILIHSLYKDREVFLRELLSNASDA
                     LNRLRFEMVTNQNILDSNAELQIRIKTDKDARMLIIQDTGIGMTKDEVIENLGTIAQS
                     GARKFLDATKDKKVDFTQVIGQFGVGFYSVFMVAEWVRVTSRSFRPDANAVSWYATGE
                     DNYEVSEADMSERGTRIEIKLKEDAAEFAEEFRLKNIIHKHSDYIGFPIYMGDGTEAV
                     NKQTSLWRTSRQEIKEDQYKEFYRQTTLDFEDPLLHTHMVTDAPVQLYALLFIPAKAE
                     RGIFALRKEDGLKLYSRNILIDDYNKDLLPEYLRFVQGVVDSEDLPLNVSRETVQSSG
                     LMPKLKKVLTNQVMRELELLAKNNAEKYHTFWQEFGPYLKQGVAANLAEADAIKPLLR
                     FKTNLNPETWTSLEEYASRMKPEQKEIYYIIGEDPKSVLRSPHLDYFHSQGTEVLLLT
                     DPMDSFMLMGLRKYKDLDLKNVAQADVDTAKQSTEQPEAEKISGDEFNTLIERFKQVL
                     GDRVTDVRASTRLVQSVARLVDTDANANPDMQRVYKYLGKEYEIPKKVLELNPSHTIL
                     KNLLHQESDSELQNIIIEQIYDSALLVEGLHPDPSSIAERVQQIMEAALKK"
     gene            9312..9938
                     /locus_tag="PLE39_13320"
     CDS             9312..9938
                     /locus_tag="PLE39_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69358.1"
                     /translation="MKKQTTLFSVLLVILALFFSNTQPVGAAAPAVISAALPNPGAII
                     PVAAGKTATYSQGPHTSGFPSSWGIVNPGKVTSSIDLGYSGTVVAPISGYLAIMKDCG
                     DHQVAVVRASNGWAIGLTHIFVNTTVRNKSVAQGQVIGKTVPPPATSKGCGYGKGSHI
                     HYTLMKWVQSGTIKYTEQDIVNTYFSKWIVKNTYLDGPTRIPIGGVIK"
     gene            10047..10676
                     /locus_tag="PLE39_13325"
     CDS             10047..10676
                     /locus_tag="PLE39_13325"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE69359.1"
                     /translation="MDIQTAYNEWSGIYDTNTNLTRDLDSKLTRDLLAHQKFDSILEL
                     GCGTGKNTIFFSQIGKKVHALDFSPGMIEKARTKVSASHVRFEMADLTKHWPCQNEAY
                     DLISCNLVLEHIAELPHIFSEAARALHRGGKFLINELHPFKQYQGTKARFERGTETVE
                     LEVFVHHISEFITTALKHDFKLLHFNEYWHPQDENKPPRIATFIFEKQN"
     gene            complement(10687..11844)
                     /locus_tag="PLE39_13330"
     CDS             complement(10687..11844)
                     /locus_tag="PLE39_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69360.1"
                     /translation="MSFDEVAVLLPDLTLTLRANLSNWYGIGASIISSPDVRMREWSV
                     FVRYQIQLADLQRRAILVKIRRHPDQSISESISSPGLKEEGRNDYEALKAIENFVSTA
                     ENIPGENNNTNPYGYFSSEEKDHFCAVRPLAFFDPFSALVMEELEARPLRNMILNFKT
                     AYHAGWQEQLSNWFYRSGRWLRVYHEQLGKLHEGPFFSDDIHAALEKSINVIEPFHDF
                     DFTLVRNVLRQTRAAYKDHNLSFGLLHWNFDCANFLVAEDNRIGILDPHFIDGPIYMD
                     IAKIMTDLQTYRLQTLSYGLFIREKVMQKFHRSILQGYFGSEQPDEVAINLYCIYAVL
                     DKWQIDEEAEHEHGNEHGWLHQFIQSHATWRRKYFMKLLSAYVDRLQKLLP"
     gene            complement(12136..12723)
                     /locus_tag="PLE39_13335"
     CDS             complement(12136..12723)
                     /locus_tag="PLE39_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69361.1"
                     /translation="MESSKRLKIFLACITLGPLFGYLLTSFWFAGGGTFWQPIDYFPH
                     PVETILNLQPYGNEFWVTTTDGVTYHILYPCQEAAGCWQEDEAVSDLVPSDAYVVSEG
                     RCESDYFLYPLFQQEIKSCVTSTIFAETPQGSSISTIASPWRVSLALTEDKKLWIWQK
                     PWSFPDRVLAFKVFFTALGLLAGTMVDTLFTRKYK"
     gene            complement(12827..14479)
                     /locus_tag="PLE39_13340"
     CDS             complement(12827..14479)
                     /locus_tag="PLE39_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNE69362.1"
                     /translation="MTLNDQYQKWKEVTLKKSLDKFKERKDRFEYSSGIEVPRLSLPP
                     TDDSAYEEKLGFPGEYPYTRGVQPTMYRSRFWTMRQYAGFSTAEESNKRYRYLLAQGQ
                     TGLSVAFDLPTQIGYDADDPIAQGEVGKVGVSISSIRDMEQLFDQIPLEKVSTSMTIN
                     APAGVLLAMYIAVAKRQGADMRQLRGTIQNDILKEYVARGTYIFPPAPSMRLITDIFQ
                     FCAAEVPFWNTISISGYHIREAGSTAVQEVAFTLANGMAYVEAALKAGLNVDDFAGQL
                     SFFFNAHNNLIEEVAKFRAARRMWARIMRERFKAQKPASWQLRFHTQTAGSTLTAQQP
                     ENNVVRVTLQALSAVLGGTQSLHTNSMDEALWLPTEKSVRVALRTQQIIAYESGVADS
                     IDPLAGSYLIEHLTDEIEKGALDYIAKIDEMGGALQSIERGYMQNEIQNAAYAAQQAV
                     ERKEQIVVGVNQFSVDEVLTLERLKVDPSIELGQREKLKKLRDARDSGRVAALLGKLT
                     EAAKGTENLMPLFIECVENDITLGEVCNALRGVWGEYVAEGF"
     gene            14697..15740
                     /locus_tag="PLE39_13345"
     CDS             14697..15740
                     /locus_tag="PLE39_13345"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HNE69363.1"
                     /translation="MTSNLTANLTIQTAQSINEIDRQTWNALSTGLPFQSHRWYQFGE
                     RVMYDCKPTYLLAYHQNELIGRAVLWKISSEPLPLSPGIGRTITETTLRRRPLLICRS
                     PLSNASGLILPKNSLRVETRNALSEIALQISRQGNCLALVFDFLDEGDSQGWEGDFVP
                     VQLSDPGTVMHSRWASLEEFLESGNKKDRQHFKRTLREAEKLGITIEKHSKVNDVEAA
                     LKLIRNVERHYKNSPNPWMRSLLENLEQVQGTWLEAKQNGNLVGCGALFDDNEAQLTT
                     ALGLAENIPYVYLLLTYASLEDAFQKKLPVLRWGSGAYDVKKSLGFELEENNHIVVSS
                     TSLIIRTLIRSLR"
     gene            15744..16661
                     /locus_tag="PLE39_13350"
     CDS             15744..16661
                     /locus_tag="PLE39_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69364.1"
                     /translation="MSILIPTLAEFMQLPTEEVANIVRSKGSKAIVFPFNGTRRWFLL
                     EHGRTDHENPAQAYVNITAQAYIATYKLVFDLGLDTIIAPVFGGEILNRGEEYMREIG
                     TGMTLLAEHPAFLEFYEQYDTRVHFYGEYRKQFDPTPYAYVSETFDKATQQTAHHKQR
                     RLFYGVCAEDATVTIAELSVRFFQKTGRTPTREELIESYYGEHIEKADIFIGFEKFNV
                     FDYPMLGWGEESLYFTVAPSLYMNEYQLRNILYDYLYLRPVQDPDYFTMPQSDFEQMR
                     RFYQDNREEVYGIGEMRGGIWYASTKITK"
     gene            16710..>17071
                     /locus_tag="PLE39_13355"
     CDS             16710..>17071
                     /locus_tag="PLE39_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69365.1"
                     /translation="MSEGTMPNTAYDTAWVARLGDIDSEMSRHALEWISENQLEDGSW
                     GAKNVSYYHDRVVSTLSAMIALTQQGKRTHDKKQIERGLLALERITDNATQGLSSAPS
                     GPTVGFELITPTLVAEAE"
BASE COUNT         4761 a         4408 c         3945 g         3957 t
ORIGIN      
        1 acacctttga ggcgactgaa tcggggctga ttagaaagag aaagacatcg gcttcctcga
       61 tccctttttc cacttcccgc caccaatcca cggtcggcgg aatgccttcc caatccaccc
      121 agaaatcaag ttcgcttttc tgcaactcgc ctgtgagttt ctttgcaaag tcaatgtcct
      181 tgcgcgaata tgatatgaat actttcgcca tgatgtattc cctccgctgt tgagtcaagt
      241 tttaatgaca aaaaagaccc taaaggtttg aaacctttag ggtctgtaaa ctaattcaac
      301 ggcaatccca aatctgctaa tttttccttc gtcggccagc ctttttcatc ccaaccgcgg
      361 agtttgtaaa actcgggcag cattttgtcg agttccacca ccttgccttt ggcggggcca
      421 tcgggcagcg gttcgtgcaa catacggtgc ggaagggtat cttctgtgga accagctttc
      481 agcaggaaca tgcgttcaag tgtgtacaca cggtcagcgg ctctcagtgc ttcttcggcc
      541 gtgtacccag cgcccgtgga gagattcatc atgttcgcca gcgtttgcgg caggatcatg
      601 cgatctttat tgaacatgta acgaataccg acaaacacac acacgccgga agaatccatc
      661 aacgcgaaag aatcttcaaa gtgcttgacg agttcaggct taccttcagt tgtgagcgga
      721 ttttccttca caggcacgcc aaagacttct tcgtatgcca cgtcaccttc catgtgggaa
      781 gcgcccttgt tggaggtggc gtacagcaag cccatccctt gcgagccgcg cggatcgtac
      841 ccggggaatt cctgcttgcg agtggtgacg gcgatttcgg ggtggccata tttttccgca
      901 aggcgatagc tgccctgggc aagttcctta ccaaagcctt cgttgtacgc catcagcttg
      961 atcattgcga tcatcgcatc gtggtcacca aatttcagcg gctgaccgat cacctcttct
     1021 ggaatgtatc ccttctcata catttccatt gcggcggcaa tggtcatgcc cgtggagatc
     1081 gtgtccatgc cgtattcatt gcacagataa tttgctttca ccaatgcggc aaggttgctc
     1141 accccacagc ctgttcccag cgacgaaatg gtttcatatt ctgggccttc ccctttgcct
     1201 ttgtagggac catcgggaac ttccgtgaga cgcccgcacg aaagcggaca gcgataacaa
     1261 ggtgtgtggc gaatcagata ttgattcttg agcgcatctc catccacatt gtgaacatgc
     1321 tcaaaagtcc cttgcaggaa attatgcgta ggcaaaatgc ccagggtatt cgtcacttgc
     1381 ggcacataag aagtgccata aatgcgcatg ttcgaaccct tcttcacatc tgcgcctacg
     1441 ttcttggagg tttccacact tagggtgcgc attccctcgg ggtcatgcag cggcgggttt
     1501 ttactcccca tcgccaccac ggctttaaga tttttactgc ccatcacggc ccccacgcca
     1561 gaacgggcag ccgcacgatg tttatcgttc atgatcgccg ccatcaacgc aaggttctca
     1621 cccgcaggcc caatacatgc gactctggct tcttcggatg tttctgcctt taggatatcg
     1681 gtagtctcat gcgtatcctt gccccaaaca tgcgcggcag acctaacctc aacctggtcc
     1741 tcattcacaa acagatacac aggctcaggc gacctgcctt caaaaatata cagatcaaaa
     1801 cccgtgcgct tcatccaggt cgggaattcg ccgcccgaat tggaatgagc cagcgcaccc
     1861 gtcaaaggag atttggtaac caccatgtaa cggttgcctg tcggtgcggg ggtggcggtc
     1921 agcggacctg tggcaaagat cagcttgttc tcaggtgaaa gcggatcagc catcgggtcc
     1981 atctctttat aaagataatg gatcgcccat ccgcgcccgc cgaggtaatc acgggcgatc
     2041 tttgggtcca catcttcggt ggtgacttta tgagtggtga gatttactcg caaaattttg
     2101 aggtgccagc cgtacatagg gttctcctgt ggaaaaagat gggtaggaag ggtgtagtta
     2161 caggttgaag ggtttttctt tgcgcttctt cgtatccttc gcggtcaacg attctatccg
     2221 ccagcgattg ccgaaaatgc agtgaccata tcgccttctt gaagcggggt attcatgtcc
     2281 accgtcaggc cgttcaacac gatgacgctt tcatcgttga ggggaatatc aaagcgtgaa
     2341 atggcatcat acaccgtcgt cccatcaggg acattcaact caacgacgcc gtgttccgcc
     2401 tcgggcggca gggctgcacg ataattggca atgagtttga cgcggatcat catttgtgaa
     2461 ttttagcatg aaagcggata aaataattgg catgaaaaga caaattccct accatggctg
     2521 gtgttttgtg tgcggaaatg aaaacccacg cggcatcggc atcaccatgt ttgtggatga
     2581 caacggcatc ctcacatctg aatttacgtt gaacgaaaca catcaaggcc cgcctggata
     2641 ttctcacggc ggggcatccg ccgccatttt ggatgaggtg atggggttgg tggtttgggc
     2701 ggcaggtcac aaggtggtgg cggcgaatat caacatcaac taccgcaaac cacttccgct
     2761 gcatcagccg cttctggcag aagcacgcat cagcgaggtt ggcgaaagaa agatcaccag
     2821 cgtaggagaa attcgtttgg ctgattcaac tgtcgccgtg agtggaagcg gggtgtacgt
     2881 cgtcgcaccg catttattcg aagcaaaacg tctaaaagga gtcgtgtcat gaaaaccatc
     2941 ggcctgatcg gcggcatgag ctgggagtca tccattgagt attaccgcat catcaacgag
     3001 accgcaaagg aaaaactggg cggtctgcat tcggcaaaaa gtttgatggt gaccgttgat
     3061 tttgcagaga tcgaaaaact gcaacacgaa gaccgctggg atgaagccgc ccaaattctg
     3121 gtgcaatgtg cgcaagacct tgaacacggc ggcgccgatt ttattgtgct ctgcaccaac
     3181 accatgcaca aactggccga ccagatcact gccaatgtaa gcatcccttt tttgcacatt
     3241 gcagatgcaa ccgctgaaaa gatccgtgcc gcaggcttga aacgcatcgg gctgctcggc
     3301 acacgcttca ccatggaaca tgatttttac aagggccgcc ttgttcaaaa cttcggtctt
     3361 gaagttttga ttcccgaaca aacagatcga gacattgtcc atcgcgtgat ctacgatgaa
     3421 ctcgtgcaag gcaagatcgt ggaaacatca cgggaagaat ataaacgcat catcgagtgg
     3481 ctgatcgaac agggtgctga aggcatcatc cttggctgca ccgagatcga attgttggtc
     3541 aaacaaaacg acagccgcgt gccgctcttc cccactacgc tcatccacgc ggtggcggct
     3601 gtggaaaagg ccttgcagga ggattgaccc ctcatgagaa ttttccacgc gatattcttt
     3661 tccctgctct tgagcgcgtg cctgactatg cccgggcctg ataccacccc cacagcagat
     3721 tcgccctttg tcacggtgca agaggaaaac ccttattcgc ccaaacccga agacctcgga
     3781 aaacaacaag caagtgtcat cgtcacttct gtagatctct ccgaacgttc agacctctct
     3841 cctgtacggg tggaggtcag catttttggt tccatgccca gtgtgtgcaa cgaactgcgc
     3901 gtcaccgtag acccgccaac cgcagcttac gaggttcata tcgaggccta cagcgttgcc
     3961 gacccaaatc aaaattgcga gaacgtcttc cagcaatttg aagggcatat cctgctcggc
     4021 gtgtacaccc caggacgata caccatttgg gtaaatgata cctttgtggg agatatgatc
     4081 tccaattaat tgaccatatt tcccattcac gccacctaaa atctggatat actcctgcca
     4141 gcccaaaaac aggagatatc aatcgtgttc aattttttca agaaaaaaga aaagaaacca
     4201 gaaacaagcc ttgctgatgc attgcgtgat gtgatcttcg gtgttgcccc tgcaactgaa
     4261 tggcccaagg gagatacagg tgaacatccg tggtcggttt tcattgaagc gcgccgacac
     4321 atcatggaaa gacaaaattt cgaagaagcc gcaaaacttt acaagcagat cacagaaacg
     4381 cccggacttg agtctcgtca ctatttacag gcatggttct tccttcgcaa gatgaacatt
     4441 cagccgcctg ccgatatggc acggaaagcc tacggcatcg ttgtagatgt ccgcttgcaa
     4501 agtggacctg aattggtggc ttgttatgcc gaccataccg cccgctacct gcatagctct
     4561 gggggcggaa tcgtttggga ggcaccagat acctccctga acgatgttat tgatgcgctg
     4621 atccgcgcag gagatgaggt ggcatcgaaa gtcccgccgt tggaaaacaa tatcaggccc
     4681 aacgccccgc agcaagtgga tgcggtccag atcggtgtac tgacacccag cggcatccac
     4741 cacggcattg gaaccttcga aggctggtcc aaagacccca tgggcagcac gatcgtaaac
     4801 gcagccagtt atttgatgca aaaccttgtc aacaagaaaa aatcatagga atttaaaagc
     4861 tccacatatc ttgaaaatgt ggagcttttt ttcgcgtcga tgttttcatc cgtctgcgca
     4921 aagatctgat cgcaattaag tcgaagcaga gagcgggaaa tacttatcct tgaggtcatt
     4981 gatgggtttt tcaaagacct gataactaag gcttgcaacc acggcagtta ccacaaaaga
     5041 aattccatac aagaccatat tccccgaatc aatggaaccc aatttgagaa ccagccaaat
     5101 ggcagccgtg tgatacacgt atagcccata cgatatcttt ccaagatacc gcagcggggg
     5161 caggtcgagg gtatggttga aaagtcctgt cttcgccacg ttatagatca aaaccataaa
     5221 caggtaattc aacaacgagt atccccagat ctgcatgtaa ccaactgaga atagaaatcc
     5281 ctggccgagg gtactccaaa gcgcacgacc ttccacaagg tattgagtcg acaggccgat
     5341 tgcagggaca aggatcgaag caagaaacag ttgaagccgc ggacggggaa tctcaaactg
     5401 ggaggcaaag gccccaaacg caaaggcatc aatatgcgaa aaaggcatgg cataaacggc
     5461 aatatcggga atcgtcgtca tgaaaggcag cctatgataa ctgtagaccc aatacacaac
     5521 tccgcgcatg atcggtccca atgcaattgc gccaaagaac aaccgcttca ttttctcatt
     5581 gggtgtcaga aacacgatca agggccacag gatatagaac tgctcctcaa cagaaagcga
     5641 ccaaaggtgg gtaaagaaac gcgaatgttc gtaggcgctg gtggtatgga agaagtcata
     5701 gacataaaaa atggaaggcc atacctggag ccagaattta tcgccgaact cattcaaaag
     5761 cggcttgaat tccaaaacag gtgcggcaaa cttcaagcca aacatgagga agatatagaa
     5821 ataatacaac ggcacaatgc gtaaaaaacg cctgccatag aacttggtga aatactcccg
     5881 tttccccaga gaattcttca tccgtatcaa aatccctgtg atgagaaaac ccgaaagtac
     5941 aaaaaataac tgcaacccca cccagccaaa gctcaaatta tcagtatgag caccaagaac
     6001 caaaagaaaa gcaaccgccc gcaagccatc gagacctgga atcataagcg tgattatacg
     6061 tgctgaaatt caaggctaca agaagttttt acggacccca acggggctga taatcgcaat
     6121 aatcgttatc tgtcaagcgg cgcagatctg taccatccac tcgcatgatg tagatctcac
     6181 agccgtggac ttcgaaacgg tcaaagtagg cagtaaacgc cacccattcg ccatcaggag
     6241 aaaaggaagg tccctgcgaa tttccgccag cgggtgaaat ctgctgcagg tcagagccat
     6301 caggattcat ggaaaataat tcccgttccc agggttttcc tgaatacgtg acgatcagcc
     6361 ctgccgcaga ccagtcactt cgtccacgca gggcaggtaa atgactcacc tggcgcaacc
     6421 cactgccatc gaggttgatg ataaatagct gacttaagcc atcagcatag gaagcgaaca
     6481 ggatctgcgt cccatctggc gaccaggttg ggtcccatgc gcgtacgtcc gatacctgtc
     6541 gcgggttgcc gccatctcga tccatcaacc aggtggtggg gtgttcagga tcgtcaaaga
     6601 agcgtgtaaa agcgatcgaa tttccatcgg gtgaaacttc aggcgcgttc aggtcgccaa
     6661 gagcaaaagt cagggctttg ggttcgcccg cagccagatc gaattcatag atctcgtaat
     6721 gtttgctctg ttcatccagg gctgaataca atacactgag cccgtcaggt gaaatggatg
     6781 gataaaagtg gcgggtggca tcttcggtgg tcaggcgctg atagttcgtc ccatcagcat
     6841 tgaccatgca gatctgttcc cgttcaagct ctttgaagac ctgacaggta aaggcaattt
     6901 tccctgttgg ttcggaaggc tcttcaacca ctggagcagt tggcgctgct gtatcaacgg
     6961 caactggcgt actggcctgc atggtttgaa acaaatgcgg ggtcggcaca ggggttgcca
     7021 ctggcagatt gcaagccgcc acaagaaata aaattgaaag gtggatcaag gaaagttttc
     7081 tcatattcaa gctttgtaaa cggcattgat ctttctctaa caaaactctt actgacaagt
     7141 ctgtccgtac gatataaaat tttacacgca accaagatca tcaatccgca tcatattgcg
     7201 ggtgaaattc aatcaacact caggaggcaa ccatggcaaa cgatgcacct cagcaataca
     7261 ctttcaaggc tgaaacaaaa caattactga acatcctcat ccattcattg tacaaggatc
     7321 gggaggtttt tctgcgggaa ctgctctcga acgcatccga cgcgcttaat cgcctgcgct
     7381 ttgaaatggt caccaaccaa aacatcctcg actcaaacgc ggagctgcaa atccgcatca
     7441 agaccgacaa ggatgcacga atgctcatca ttcaagacac gggcatcggc atgaccaaag
     7501 acgaggtcat cgaaaatctg gggaccatcg cccaatccgg tgcgcgcaag tttttggatg
     7561 caaccaaaga caaaaaagtg gactttacac aggtcattgg ccaatttggt gttggctttt
     7621 attccgtatt catggtggca gaatgggtac gggtcacctc ccgctctttt cggccagatg
     7681 ccaatgcagt tagctggtat gccacaggtg aggacaacta cgaagtcagc gaagcagata
     7741 tgagcgaaag aggaacgagg atcgagatca agctcaagga agatgctgcc gaattcgcag
     7801 aggaattccg acttaagaac atcatccata aacactcgga ttatatcggc ttccccatct
     7861 atatgggcga cggcactgaa gcagtcaata agcaaacctc gctctggcgc acttcgaggc
     7921 aggagatcaa ggaagatcaa tacaaagagt tttaccgcca aaccacactt gatttcgaag
     7981 acccgctttt gcatacgcac atggtcactg atgcgccagt gcagttgtat gcactcctgt
     8041 tcatccccgc caaggccgaa cgcggaattt tcgcactgcg aaaggaagac ggcctgaaac
     8101 tttactcgcg caacattctg atagatgact acaacaaaga cctgctgcca gaatatctgc
     8161 gtttcgttca aggtgtggtg gactcggaag acctgccgct caatgtctcc cgtgaaactg
     8221 ttcaatcaag tgggctgatg ccgaagctga aaaaagtcct gacaaaccag gtcatgcgcg
     8281 aattggagtt gctggcaaaa aacaacgcag aaaaatatca cacattctgg caggagtttg
     8341 gtccatactt gaaacagggt gtggccgcca acctcgcgga agcagatgcc atcaagccgt
     8401 tgttacgctt caaaaccaat ctaaaccccg aaacatggac ttcgctcgaa gagtacgcat
     8461 ctcgaatgaa acccgaacaa aaggaaatct actacatcat cggcgaagac ccgaaatctg
     8521 tgctgcgcag tccgcacctt gattatttcc attcacaggg aaccgaagtg ctgctgctca
     8581 cagacccgat ggattccttc atgttaatgg gactgcgcaa atacaaagac cttgatctga
     8641 agaatgtcgc ccaggccgat gtagacaccg caaaacaatc aaccgaacaa cccgaagcgg
     8701 aaaaaatctc aggcgatgaa ttcaacacct tgatcgaacg gttcaagcag gtgcttggcg
     8761 accgcgtcac agatgtacgt gccagcaccc gccttgttca atctgttgca cgccttgtgg
     8821 atacagatgc caacgcaaac ccggatatgc agcgtgtgta caaatacctc ggcaaggaat
     8881 atgaaattcc aaaaaaagtt ttggagttga atccatccca caccatattg aagaatttgc
     8941 ttcatcagga atcagattca gaattgcaaa acatcatcat tgaacagatc tatgatagtg
     9001 ccctgctggt ggaaggtctc caccccgacc catccagtat tgcagagcga gtacagcaaa
     9061 tcatggaagc agcactaaaa aaataacggc acacatacgc aactaaaaag ataaaaactg
     9121 tcgctatttg tgtaaatcgg ttgtaaagca ttgatattgt gattttcatt acaatatcaa
     9181 tgcttttatt tttaaacctg aaaagaaata cattttgtta ccaaattgtg acaaggctgt
     9241 gacatccccg taacaatggg gcgctataaa taaagtatgg aaaacaacaa acaaaaaagg
     9301 agacaatgaa aatgaaaaag caaaccaccc tcttctctgt gctactcgtc attctggctt
     9361 tgtttttctc aaacacccag ccagttggcg cggctgcacc agccgtgatc tcagccgccc
     9421 tgcccaaccc tggcgcgatc attccagttg ccgctggaaa aaccgccacg tactcacagg
     9481 gaccacacac ttcgggcttc ccatccagct ggggcattgt aaaccctggt aaagtcacca
     9541 gcagcattga ccttggctac tccggcaccg ttgttgctcc gatcagtgga tacctcgcca
     9601 tcatgaaaga ttgcggcgat caccaagtgg cagttgtccg cgcctcaaat ggctgggcca
     9661 tcggcctgac acacattttc gtcaacacca cagtaagaaa caaatcagta gcacaggggc
     9721 aggtgatcgg caaaaccgtt ccgcctcctg caaccagcaa aggctgcgga tacggaaaag
     9781 gctcacatat ccactacacg ctaatgaaat gggtccagag cggcacgatc aaatacactg
     9841 aacaagacat tgtcaacacc tatttttcca agtggatcgt gaaaaacacc tatcttgatg
     9901 gtcccaccag gatccccatt ggcggtgtga tcaaataaaa agcgcaaact ttagcaaagc
     9961 aggcatccat atacaggatg cctgttttgc tcttaaaaca aactacaatg tggacaaacc
    10021 aaatttacag gaaccgaaat aatcaaatgg atattcaaac cgcatacaac gaatggtcag
    10081 gaatctacga cacaaacacc aacctgaccc gtgatctcga ttcaaaactg acccgtgacc
    10141 tgcttgccca tcaaaaattt gactccatcc tcgagcttgg ctgcggaaca ggcaaaaaca
    10201 ccatcttctt cagccaaatt gggaagaaag tacacgcact ggacttctca cctggcatga
    10261 tcgaaaaagc cagaacaaag gtcagcgcaa gtcatgtccg ctttgaaatg gcagacctca
    10321 ccaagcactg gccatgtcaa aacgaagcct atgacctgat ttcctgcaat ctggttttgg
    10381 aacatattgc tgaactcccc catatttttt ccgaggcagc acgcgcgttg catcggggcg
    10441 gaaaattcct catcaacgag ctacatccct tcaaacagta tcagggcaca aaagctcgtt
    10501 tcgagcgcgg cactgagacc gttgaactgg aagtctttgt acatcacatc tctgaattca
    10561 tcaccaccgc cctgaaacat gatttcaagc tactccactt caatgagtac tggcatccgc
    10621 aagatgaaaa caaaccgccc agaatcgcaa cttttatttt cgaaaaacag aactaagggc
    10681 aaagtctcaa ggaagtaatt tttgcagcct gtccacataa gcggaaagca atttcataaa
    10741 atacttccgc ctccaggtgg cgtggctctg aataaactga tgcagccatc catgctcgtt
    10801 cccatgttca tgttccgcct cttcgtctat ctgccattta tccagaaccg cataaatgca
    10861 atagagattg atcgccacct catcaggctg ctcagaccca aaatacccct gcaatatgga
    10921 tcgatggaac ttctgcatca ctttctcgcg gataaacaaa ccataggaca gagtttgcag
    10981 gcgataggtc tgcaaatcgg tcatgatctt ggcaatatcc atgtaaatgg gaccatcaat
    11041 aaaatgcggg tccaatatcc caatacgatt gtcttcggca actaaaaagt ttgcacagtc
    11101 aaaattccaa tgcaacaaac caaaagacaa gttgtgatcc ttgtaagcgg ctcttgtttg
    11161 cctcaaaaca ttacggacaa gtgtaaagtc aaagtcatgg aatggctcaa tgacatttat
    11221 gctcttctca agagcggcat ggatatcatc gctaaaaaac ggtccttcgt gcaacttacc
    11281 caactgctca tgatacacac gcaaccagcg tcccgaacga taaaaccagt tacttaattg
    11341 ttcctgccag cctgcatggt aggccgtttt gaaattcaat atcatgttgc gcaatggacg
    11401 tgcttccagt tcttccatga caagggcgct gaaggggtca aaaaatgcca atggacgaac
    11461 tgcgcaaaag tggtcttttt cttcactact aaaatatccg tatggattgg tgttgttatt
    11521 ttcccccggg atattctcag ccgttgaaac aaaattctca atggctttca gagcctcata
    11581 atcattccgt ccttcctctt tcaaaccagg gctgctaatc gattctgaaa tactttgatc
    11641 agggtggcgg cgaattttca ccagaatcgc ccgcctctgt aaatctgcca gttgaatttg
    11701 gtaacgcaca aaaaccgacc attcccgcat ccgcacatca ggggaactta tgatcgatgc
    11761 accaatacca taccaattag aaagattcgc tcgcagggtt aaagtcagat ctggaagcaa
    11821 aactgcaact tcgtcaaaag acacaaaacg ctcctttaaa aaccaataaa ttaaaacaaa
    11881 gctacatcag gtctctggtt ttattgtatc agcaggaaaa gaacaccctg cgctacttgc
    11941 atttcaacaa tatattaatt attttgattc cttttcgtcc actttgtagt gtcatttata
    12001 gtagcatggt gaacttggga aacacactcc accattagtc cttgtcaatt tggaattttt
    12061 taccagatgc ccacctttag atatcaatat caccgcccag tttctgaaag ccctcccaca
    12121 acctgcaaag atttgttatt tgtatttccg agtaaatagt gtgtcaacca ttgttccagc
    12181 cagcagccca agcgctgtga aaaagacctt gaaagcaagg actctgtcag gaaagctcca
    12241 gggcttttgc cagatccata atttcttatc ctctgtcaga gctagcgaga ccctccaggg
    12301 acttgcaatc gtactgatcg aactgccttg aggagtctca gcaaatatgg tggaagtgac
    12361 acaggatttg atctcctgtt gaaacaaggg atataaaaaa taatcacttt cgcatcggcc
    12421 ttcactgacc acatacgcat cacttggaac aaggtccgaa accgcctcat cctcctgcca
    12481 acaacctgcc gcctcctggc atggatacaa aatatgataa gtgacaccat ctgtggtcgt
    12541 cacccaaaac tcattgccat agggttgtaa attgagaatc gtttctacgg gatggggaaa
    12601 ataatcgatc ggctgccaaa acgtcccccc gcccgcaaac caaaaactgg ttaacaagta
    12661 tccgaacaat gggcccaagg tgatgcaagc cagaaatatt ttcagtcttt ttgaagattc
    12721 cataaggcgg atattttatc tcaaacgaac acgggggttg ctttttcaat aaaaacggga
    12781 cagaaaatag caggaacttt tctgtcccgt tgtagaagtg accgggctaa aacccctccg
    12841 ccacgtactc accccacacg cctctcagtg cattacaaac ctcaccgagg gtaatatcat
    12901 tttccacgca ctcaataaag agcggcatca aattttccgt ccctttggca gcttcggtta
    12961 attttccaag caatgctgca acccgcccac tgtcacgtgc atcacgcaat ttcttcaact
    13021 tctcccgttg acccagttca atggacgggt ccactttcaa gcgttccaga gtgagaactt
    13081 catccacgga aaattgattg acacccacaa cgatctgctc tttgcgctcg acggcttgct
    13141 gggcggcata ggcggcattt tgaatttcat tctgcatgta accgcgctca atggattgca
    13201 atgcgccgcc catttcgtcg attttggcaa tatagtcaag cgcacctttt tcgatttcat
    13261 cggtcaaatg ctcgatcagg tatgaaccag ccagcgggtc gatcgagtcg gctacccctg
    13321 attcgtaggc aatgatctgc tgtgtacgca atgccacgcg gacagatttc tcggttggca
    13381 gccacaacgc ctcatccatg ctgttggtgt gcaggctttg tgtgccgcca agtactgcag
    13441 aaagtgcctg cagggtcaca cgcaccacat tgttttcagg ttgctgcgcc gtgagtgtgg
    13501 agcctgcggt ttgagtatga aagcgcaact gccacgaagc aggcttctgt gctttaaatc
    13561 tctcgcgcat aattcgcgcc cacatgcgcc gcgctgcgcg gaatttggca acctcttcga
    13621 tcaggttgtt gtgggcattg aagaagaacg agagttgacc agcaaaatcg tccacattca
    13681 aaccagcctt gagggcggct tccacatagg ccatgccatt cgccagcgtg aaagccactt
    13741 cctgcacagc agtggaccct gcctcacgga tatggtaccc cgagatcgag atcgtattcc
    13801 aaaatggaac ttcagcggcg caaaattgga agatatctgt gatcaaacgc atcgaaggtg
    13861 caggcgggaa aatatacgtt ccacgcgcca cgtattcttt gagaatatca ttctgaatcg
    13921 tgccgcgcag ctggcgcata tctgcaccct gacgttttgc cacggcgata tacatggcca
    13981 gtaaaacacc tgcaggcgca ttgatggtca ttgatgtgga aactttttca agggggatct
    14041 ggtcgaacaa ctgctccata tcacggatgg aagaaatgga aacacccacc ttgccaacct
    14101 caccctgtgc gatcgggtca tcggcatcgt aaccgatctg ggtcggcaaa tcgaaggcca
    14161 cactcagccc cgtctgcccc tgtgcaagca ggtagcgata ccgcttgttg gattcttctg
    14221 cagtggaaaa gccagcgtac tggcgcatcg tccaaaagcg tgaacggtac atcgtcggtt
    14281 gaacgccgcg ggtataggga tattctccgg ggaagcccag tttttcttcg taagcagagt
    14341 catctgtcgg cgggagagag aggcgcggca cttcaattcc agaagaatac tcaaaacgat
    14401 ccttgcgctc tttaaactta tccagggatt ttttgagagt gacttctttc catttttgat
    14461 attgatcatt gagggtcatg ggtaagtcct ttttaaccac gaaattttga ggggtaaatc
    14521 catgctcaag tataaatcca acctaaaatt agctgtagga caagtgacat gctgacacac
    14581 gcaaaacatc ccgatttcat attcatccac ttcagatgta ataactttca taattgttaa
    14641 cgcagggtgt gtgtgcaaat ttacgaactt tccaaaacag gcagtaaaat tacagagtga
    14701 ccagcaattt gacggcgaac cttaccattc aaaccgcgca aagtataaat gaaatagaca
    14761 ggcaaacctg gaacgcgctt tcaactgggc tgccatttca aagccaccgc tggtatcaat
    14821 ttggcgagcg cgtgatgtac gattgcaagc cgacttacct gctggcttat catcaaaacg
    14881 aactcattgg ccgtgccgtt ttatggaaga taagcagcga gccgcttcca ctctcaccgg
    14941 gaattggtcg caccattact gaaaccactc tgcgccgacg gcctctgctc atttgccgtt
    15001 ctcctctttc caacgccagc ggattgatcc tgccgaagaa ttccctgcgc gtcgaaaccc
    15061 gaaatgcact cagtgaaatc gctctacaaa tcagccgtca gggaaattgc ctggcactcg
    15121 ttttcgattt tcttgacgaa ggggacagcc agggctggga aggtgatttc gtgcctgtac
    15181 aactctcaga cccaggcact gtgatgcaca gccgctgggc cagccttgag gagtttctcg
    15241 aaagcggcaa caaaaaagac cgtcaacact tcaagcggac cctgcgtgaa gcggaaaagc
    15301 tcggcattac gatcgaaaaa cacagcaagg tcaatgacgt tgaagccgcg ctcaaactca
    15361 tccgcaatgt ggaacgtcac tacaaaaatt cgcccaaccc gtggatgcga agcctgctcg
    15421 aaaatcttga gcaggtgcag ggaacctggc ttgaagccaa acagaatgga aatttagtcg
    15481 gctgcggcgc actgtttgac gataatgaag cacagttgac cacagcgctt ggtcttgcag
    15541 aaaacatccc ttacgtgtat ctgttgttga cctacgccag cctcgaagat gcatttcaaa
    15601 aaaagctccc tgttttacgc tggggcagcg gcgcatacga tgtgaagaaa agccttggct
    15661 ttgaattgga ggaaaacaac cacatcgttg tttcaagcac cagcctgatc atacgaaccc
    15721 taattcgatc actgagataa tttatgagta tccttattcc aaccctcgca gaattcatgc
    15781 aactccccac agaagaagtg gcaaatattg tacgatccaa aggctcaaaa gccattgtgt
    15841 tcccttttaa cggtacgcgc cgctggtttc tactggaaca tggtcgcacg gaccacgaaa
    15901 accccgcaca agcctatgta aacatcacgg cacaggcata cattgccacc tacaaactgg
    15961 tcttcgatct cggcttggat acgatcattg cccccgtttt tggcggtgaa atcctaaacc
    16021 gcggcgagga atacatgcgt gaaatcggca ctggcatgac cctgctggcc gagcaccccg
    16081 cttttcttga gttttacgaa cagtatgata ctcgtgttca tttttacggc gaataccgca
    16141 aacagttcga cccaacgcct tatgcatatg tcagcgaaac atttgacaaa gccactcaac
    16201 agaccgcaca ccacaaacaa cgaaggttgt tttacggcgt ttgcgcggaa gacgccacgg
    16261 tcaccattgc agagctttcg gtgagattct ttcaaaagac aggcagaacc ccaacacggg
    16321 aggagttgat cgaatcttat tacggagaac atatcgaaaa agctgatatt tttatcggct
    16381 ttgaaaaatt caacgtattc gattatccca tgctgggctg gggtgaagaa agtttgtact
    16441 ttactgtggc gccatcgcta tatatgaacg agtaccagct ccgtaacatt ctgtacgatt
    16501 acctgtatct gcgtccagtg caagacccag actatttcac catgccgcaa agcgattttg
    16561 agcaaatgcg gaggttttat caagataacc gcgaagaggt ttatggcatc ggtgaaatgc
    16621 gcggtggaat ttggtacgct agcacaaaga tcacaaaata accgaacgga gcagcaacga
    16681 tgcgtacaat gaccaggtta ctcaccgata tgtctgaagg aaccatgccg aataccgcct
    16741 acgatacggc ctgggttgcg cggttgggtg acattgacag cgaaatgagc aggcatgctc
    16801 ttgaatggat cagcgaaaat caactggaag atggcagttg gggtgcaaaa aatgtatctt
    16861 actatcacga cagagttgtc tccactctat cggcaatgat tgcactcaca cagcaaggca
    16921 agcgcactca cgacaaaaag cagatcgaac gcgggttgtt ggcactggaa cgcatcacag
    16981 acaacgccac acaagggctt tcctccgctc ccagcggacc caccgttgga tttgaattaa
    17041 ttacaccgac ccttgtagca gaggcggaaa a
//
LOCUS       DAOQJC010000090        16304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1701_length_16304_cov_8.492646, whole genome shotgun sequence.
ACCESSION   DAOQJC010000090 DAOQJC010000000
VERSION     DAOQJC010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 16304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16304
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1701_length_16304_cov_8.492646"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..636)
                     /locus_tag="PLE39_13360"
     CDS             complement(37..636)
                     /locus_tag="PLE39_13360"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNE69366.1"
                     /translation="MNKLIVIVGASGVGKTTLARALCSKQNFALAYEQHNERPFQALF
                     KNDPKYALANQMDYLLYRAEQEYEFRQGDLPALMDGGFDLDFHGFTRLFHTRGLLNDA
                     EYDLCRRFYTLTRSLLPPPDLIIALSADSQTISTRLASRNRINIASSEDANLLEEYVG
                     EWLATVPEEKVLKLDVTHENEEYTQSVPLILDTLDLLHK"
     gene            complement(644..1993)
                     /locus_tag="PLE39_13365"
     CDS             complement(644..1993)
                     /locus_tag="PLE39_13365"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNE69367.1"
                     /translation="MRHSFIRYNFILVSAILLSSLACSSLTPTPEPALTYRYDNLPNA
                     GEEDETAARFRAISQWGKTNLTYYFANGTSKINGNIERDLIRAAFQLWADETPLTFNE
                     VSDSAQADIIIGWAEGEHGDGDPFDGPGDVLAHASYPSPYQERQVFLHFDDAERWVDS
                     NTQNVDLLTVAAHEIGHNLGLDHSNDPNALMYPSYSGPHRFLGNDDVAGVQSLYGLAA
                     QPAESPETPPQGATPPPSANQDSDGDGISNTDEALRTGTDPNNPDSDADGLSDGVEVI
                     YRMNPLDADMDNDGVNDGQEVAQGSNPFFPDQTASISPELSAEVSEFLTKAIELQIEA
                     YRRGDSSIASSIMAGDIFTTLDQDITTLNGQGFAQISEIDYYNSYINDIRVINETNIE
                     VDTCEVWSTNTYRRADGKLIQSTDPRLLPQTITIQKLSQGWFITQVNFFEAPSFCNS"
     gene            2073..2543
                     /locus_tag="PLE39_13370"
     CDS             2073..2543
                     /locus_tag="PLE39_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69368.1"
                     /translation="MKEPIILKSDDPILNTIKFKPYRNMVQRRVVPFVPGEGQPQTME
                     VITPWGARLVAKKGDLLVGELNAPDDMWPVSPEIFDASYMVISPGICVKRAVTLLAPL
                     TDLTDGDEDQTVIIHTLEGVDTVRAGDFLLAKGVKGEIWPYPKAKAAEIMRPAE"
     gene            complement(2577..4979)
                     /locus_tag="PLE39_13375"
     CDS             complement(2577..4979)
                     /locus_tag="PLE39_13375"
                     /inference="COORDINATES: protein motif:HMM:NF024677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HNE69369.1"
                     /translation="MTETTPTPTAQSTPAAEAGKEAQKQAGNEAGTATTTPAPAVAAT
                     PIVASTTAEQTAKKEKEPEKSSTLSELRSSESSHPVRPEVRPHAFVVMPFGKKKGGDG
                     SLYDFNAIYKELIKPALEEAGFEPFRADEETSSGDILTDMFQELLLADLCLCDLSIDN
                     ANVYYELGIRHAFRKRGVVHIQAGRAYMPFDIFNVRTLPYHITPEGVPDPQYIKADIK
                     AIARMTRDTWASDRDAIHSPIFNLLTGLREPERTALRTPLATGFWREYNEWRNKVTVA
                     QRQKRIGDILLLTEEIQNPLIKEEAIGEAGRALSDMGRNELALAQYRKGLEVNSSNLN
                     FRRQEAFNLNRMGRVDEAIVKIEHLLNDFPNDSEAIAYLGRIYKEMWVGSWKNINDKN
                     KRIKNAFETYHWLIRAVDIYLRGYHIDLNNNYPGINAFTLSIIAVHLADKFDDPKDPD
                     PDITRIRQNLPELRGSLIFGLENITDDDRADYWTLISLAELRVLTADNVSAVARAYRK
                     AITASRRNLFFLNSSLAQLEILKALGLREEFVQVGIDTLKDEIMRTSGDDASHSTSKK
                     GRSKKTKTDARAFLFTGYMVDYPAKEKKTFPPERENEIRQEIIRKLEKFKITPDDRAF
                     VGGLSAGSEILFAEVCAEKGIKVKAFLPLPESAYIRKFVSPAGDNWVDRFHKIRNHPL
                     VDEMYQIEHVGLPKEGDDPYERNNRWALYSSLGKGIDKVYLIALWNAHGDKPKDLDAR
                     LVHHMIELMRDTGGSIEQINTSKYLHSVIDNVLGRLMDEQESGKAKSNATKVRKLSDS
                     KK"
     gene            complement(4996..6327)
                     /locus_tag="PLE39_13380"
     CDS             complement(4996..6327)
                     /locus_tag="PLE39_13380"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HNE69370.1"
                     /translation="MENQPQQNTPILEVAWTRFAQLDAMATERSKAHLRLRRWIATLG
                     VLTTFFAILTQLFAERLNPVAALILKIIFIVTPLAASALAAYVSQFYSTGDWLVARAG
                     AEEILKEIYAYRTILKGTPTRRTWLENRLGEIQRSVYRGMNGEMILKPYKGMLPPPPR
                     FDPKYPNSDPGFNDLNGDEYFNYRLDNQLSWHIKEINKRQTERTRLKIYILLAGLAGA
                     LFAAIGSSLTMWVALAAAFSAAFIGWQELRSLDSVVRNYSKVVLELNILFDHWKNLEG
                     EERDQTEFYKTVRSTEDILWSQNVEYIKAMQEALRDSDLEEEASLINRVIKEQRESDK
                     RFRGAIADAVVDHTRESMQETEETLVETYKSTLGTLAEEASSDLVQAELAAMREAAQN
                     AVENFSEKIGLSSAMQAIHDEFAGVDIDANTPMSVLNDLMSRLPKTTEVKG"
     gene            complement(6347..9379)
                     /locus_tag="PLE39_13385"
     CDS             complement(6347..9379)
                     /locus_tag="PLE39_13385"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNE69371.1"
                     /translation="MEESSRTTKIFISYSRKDKLFVRKLNAALDAAGIDAWVDWEGIE
                     LASDWMARITAAIEGGDAFVFVISPDSLKSKVCMDELELGISSNKKIIPVLYREPEKK
                     QKMHPKLASTNWVYLRPKKDDFKNTIPKLIEAIQTDLEWVQQHTRLLQRATEWDQKRR
                     NKSYLLQGTDLEDGEQWMTASTTGPNRSVNPMQAEYISTSRKTSVQRQRNLTIGVGAM
                     LVISIFFGIFAFLQRNIARQSERDAVNSRSTAVANELARATQQAIAEEQRVIAEQQKL
                     IAQENEEKAEAQRSAAEAKIYQDRDGELDTSTLLAVNALQKLEGLDDAEDILRNNITL
                     LPIPVKQMNLGARVWAIQPSADQSKFVTTDSAGKACVWSMEDGSQFFCAQHDGIVYDA
                     NFSQDGTILATGTEKGVLTFWNVDTGEQIKSIQYEGTIWDLNPHPGGRWLGVGRSNAI
                     SMIDMTDLTEALFITLSGEVKTIDFDDTGSFMAMGTSKGNVTVWAVMGNQSVGGPKHN
                     GEVIDVELSPDGNWVVSVGQDSTARSAQSRAGGQKYSVTHGDWVEDVTFGPDSSWFVT
                     ASDDNTVRVIDTATGQERLRMVHNNFVQKVRVSGDGQWIATTGYDKTLRIWDAASGAE
                     TMQIPLEGIGSSIRFNRDATRLIVGDYDGHVTLWNISELKARRGFQQFSEFVNEAHFS
                     PDGKWLAVNTDDRKIWRIDLDESGNPQGERKVIAETDGLTSDMAISADSNWVAAVEND
                     ENIADYNRVVLARADGSNEFFLSHDTQVITSTAFTPDSKQIITADEKGVIHIWNVETG
                     EKVNSLSADGIILTLAVSPNGKYLVAGIEDANHSLVWDLTTMTQVKVLEQIGKIKSVQ
                     FSNNGNLLATGSSETSISLWNAGDGSFAPVNDGFLANGEVLSLDFRPDDQWLAVGDST
                     GFVYIFDIGLHEEIARLPHVDKVTSISFSPDGSAISTAARKTVLFWGVSSIPQILSKD
                     LIENACSRLTHNFDQSKWELLFSDEEYRPICPNLAVEN"
     gene            complement(9398..10777)
                     /locus_tag="PLE39_13390"
     CDS             complement(9398..10777)
                     /locus_tag="PLE39_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69372.1"
                     /translation="MFVTKNLYQNDETWKSTPLGNSSETIGGWGCLLTSVTMMLNGIG
                     YNETPVTANEKFKAAGGFSGALFMPSYLPYVWPNCAYRDMQPCEAYPAPIPQIDAAVA
                     AGKPVILQVDWNKQAGIQTHFVLVKEKKGDDYVLYDPYKYSGDGPDKEVLLTTRYKYN
                     GAKLTSEISAVLWFDSYSSTPPEPPKVTKVPVPAEKYLLYAGEDDLALRSEPSVSGYL
                     WKRMLLGTELICLEDKAKAKSKIGVQGQWIQVQDPKGDQGYVAAWFVSEQKGKPAAST
                     TTTSTPAAAPAPVAAAPAPVPAGALALYPTEELSFRSQPVISPETLVRRVPVTEQLIC
                     LEAANTAIPKVGVQNQWLKVRGADKKEGYVAAWYVKYAGGSTAQATATSAPAATGSKK
                     VKTTVEMVSLRNQPVVSDATLIKRVPINYEFTITEAGGEAKVGKNDQWIKVKDATNEG
                     YVAAWFVSF"
     gene            complement(10968..11708)
                     /locus_tag="PLE39_13395"
     CDS             complement(10968..11708)
                     /locus_tag="PLE39_13395"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNE69373.1"
                     /translation="MRIAFISDIHGNFTALQAVLDDIKVQNIDEVICLGDTVSLGPQP
                     REVLDRLRELNCVIIKGNHDSAILDPEEAEKFEITSHLIPDLYWCRERLTKNDLDFIA
                     SFKTLHEISMPNGVNILCFHGSPLSPTDIIQATTPPETLDKYFEGHQATVFIGGHSHI
                     QMYRRHGDKLILNSGSVGNSFMFAFTPGNAPRLLPWAEYTIISQNGNSLDVDLRRVYF
                     DTTVLMEAVQASGIPSAEWWFRQYATTK"
     gene            12036..13733
                     /locus_tag="PLE39_13400"
     CDS             12036..13733
                     /locus_tag="PLE39_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69374.1"
                     /translation="MTEIYLGALVEVTGRQEDVPMLGKDGQTYTSTWVECVYRNSQGT
                     VAGWVRDEFFDDYVEKFPPPVVEIPAATEEQMGEFPYATKNPNDPAQYLVLGRNSKGE
                     EQVKYNLCAEFCVAFVMGVGIKQFLRDWENSAGSLYKWALGGDSDKTSDLSLVKNMLN
                     PYSYSAANGNIIDFAVTLIGPAFEDKTLSPGRFQKMLQTHSLLAQVVINKNTGKLIPN
                     NETKRANGGINHWIVLESATPNGTDGGRVEIYNPFNNQRQEYSYEYFISSFGGGTYTG
                     CWVKRRSEMVAVKAMDEVASAKKWCIAPGTFRQTPDGVGVLSFMKGAVMESSGATEVK
                     NGKTWIQVKYKSRTGWVRNSVLEDYEDRFSTPEVIIPHPTPEEDDAEQYMFLPGEDGK
                     KRNMCGQLCASFIMNIDVESFVQDWKLKARPYYQVSIAGKNDNGTGIDSLETMFRASP
                     YNAAPGDVFRFELGMKDPVTGILILSAGRMKKMLETHYLVAGVRIRQSDKLTGRLSGQ
                     GIGHWVVVDKVFPNGKNSGNGGWVEIYNPYPNRRQEYAYDEFMQSFAGTLGLWVRRK"
     gene            complement(13739..14614)
                     /locus_tag="PLE39_13405"
     CDS             complement(13739..14614)
                     /locus_tag="PLE39_13405"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69375.1"
                     /translation="MQTPDSNEPIQLIPASRFNYEELTAIYNQTRVDYMIPMPMNAAR
                     LAEYVRIYDVDLDHSLVAATHDGQMLGVAMLGIRKKRVWLTRLGVLPNTRRSGIGSAL
                     VGGLIEDAAKLGLDFVMLEVIKNNTPAHQLFLKYGFREIGELLVLRRSPLIPPIDPIV
                     ADAQRMDRTEALVLVGHDRGTQPWTNQSESLINAHEVSGLSITLVDGSRGWLVYQREK
                     FLLTRFAIKTEAGDPATVAYALLTHLHSQYPRLDTHIENIQVNDPHLPAYYKMGYVES
                     FRRIEMWRGDFPSSN"
     gene            complement(14615..14986)
                     /locus_tag="PLE39_13410"
     CDS             complement(14615..14986)
                     /locus_tag="PLE39_13410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE69376.1"
                     /translation="MAKILVVEDIQDNFELVRRALSGTDHQIIHAPDAETGLQSALDT
                     IPDLILLDLGLPDHDGQTLAGWLRETPELANTPIVALSAWPEENAKEMVESYGFSGYI
                     SKPITRVADFIARINSFLKAQ"
     gene            complement(15120..15500)
                     /locus_tag="PLE39_13415"
     CDS             complement(15120..15500)
                     /locus_tag="PLE39_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69377.1"
                     /translation="MNNQLNLSRILLANGVFCSTSGLIFLLAAKPLSGFLNTVPVVMS
                     VLGIGLIAYGVWVIYMATRRLITNFFSIFVIGADLLWVLFSVLLLILPAFNFSAEAKW
                     AIGITAICVDLFATVQFLEWRKIR"
     gene            complement(15504..15944)
                     /locus_tag="PLE39_13420"
     CDS             complement(15504..15944)
                     /locus_tag="PLE39_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69378.1"
                     /translation="MNTKNNLNFWWRWLVTANIGVILFGLAQVLLPNLVRQGFSLMVF
                     GSASYIDSFPLDAIRYITLTHAVMGAVMIGWGVSMMYTLFTQFRKGEWHGWLSLVVAL
                     ILWFIPDTAMSIATGFWQNAVLNTVFAILFVIPLSATYQIFRRA"
     gene            16081..>16304
                     /locus_tag="PLE39_13425"
     CDS             16081..>16304
                     /locus_tag="PLE39_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69379.1"
                     /translation="MRIVNGRVALVFALAALFLLTGCGGGEKYTYRVNGLAGQAHVEY
                     ASPDGSMVEEDVQLPWSIEVDLGNSAEFTL"
BASE COUNT         3723 a         3852 c         4473 g         4256 t
ORIGIN      
        1 acttagtgcc tttgtgactt tgtggtctac aacactttac ttatgcaaga ggtcaagtgt
       61 atcaagaatt aagggaacac tttgggtgta ctcctcattt tcatgggtga cgtctaactt
      121 aaggactttt tcttcaggga cagttgccag ccattcaccg acatactctt cgagcaggtt
      181 cgcatcttca cttgacgcaa tgttgatcct gtttcgagag gcaagccttg tgctgatggt
      241 ttgtgagtcc gcgctcaagg cgatgatcaa gtctggaggc gggagcaggc tgcgggttag
      301 ggtgtagaag cggcggcaca ggtcatattc ggcatcgttg agcaatccac gggtgtgaaa
      361 gaggcgcgtg aatccgtgga agtccaaatc gaagccgcca tccatcaagg cagggagatc
      421 tccctgccga aattcatact cctgctcggc gcgatacagc aggtaatcca tttggtttgc
      481 gagcgcgtat tttggatcgt ttttgaagag cgcctgaaag ggacgttcgt tgtgctgttc
      541 gtaggcaagg gcaaagtttt gtttgctgca cagggcgcgt gccagtgtgg ttttgcccac
      601 accgctcgcg ccgacaatga caatgagttt gttcatggaa tctctacgag ttacaaaagg
      661 agggtgcttc aaaaaaattg acctgggtga tgaaccaacc ctgcgatagt ttttggatcg
      721 tgatggtttg cggcaggagg cgcgggtcgg tgctttggat gagtttgcca tcggcgcggc
      781 ggtaggtgtt ggtgctccac acttcgcagg tgtccacttc aatgttggtt tcgttgatca
      841 cgcggatgtc gttgatgtag ctgttgtaat aatcaatttc agagatctgg gcaaaaccct
      901 gcccgtttaa cgtggtgatg tcttgatcga gcgtggtgaa aatatcacct gccatgatgg
      961 aagatgcaat gcttgagtcg ccgcggcggt aggcttcaat ttgcagttcg atggcttttg
     1021 tgaggaattc gctgacttca gcagatagct cgggggaaat gctcgccgtt tggtctggga
     1081 aaaacggatt gctgccctga gccacttctt gaccgtcatt gacgccgtca ttatccatat
     1141 cggcatccag cggattcatg cggtagatga cttccacacc gtcgctgagg ccgtcggcgt
     1201 cgctatcggg gttgttgggg tcggtgcctg tgcgcagggc ttcgtcggtg tttgagatgc
     1261 cgtcaccatc gctgtcttga ttggcgcttg ggggaggtgt tgcgccttgc ggcggtgttt
     1321 cgggcgactc ggcgggttgt gcggcaaggc catacaaaga ttgaacacca gctacatcgt
     1381 cgttgccgag gaagcgatgc ggccccgagt aggatgggta catgagcgcg ttggggtcat
     1441 tgctgtgatc gaggccgagg ttatgtccga tctcatgggc ggctacggtg agcaggtcta
     1501 cgttttgtgt gttgctatcc acccatcttt cggcatcgtc aaaatggaga aagacctgcc
     1561 gttcctgata tggactgggg taggatgcat gggcaagcac gtcgccgggg ccgtcgaagg
     1621 gatcgccgtc gccatgttcg ccttcagccc acccgatgat aatgtctgct tgagcagaat
     1681 cagatacttc gttaaaggtg agcggggttt catcagccca aagttgaaaa gcggcgcgga
     1741 tgaggtcgcg ttcgatattt ccgttgatct ttgaggttcc attggcgaag taataggtga
     1801 ggttggtttt tccccattgt gaaatggcgc gaaagcgggc ggcggtttca tcttcttcac
     1861 cagcattggg aagattgtcg tagcggtaag tgagggcggg ttctggtgtt ggggtaagcg
     1921 atgagcaggc cagtgaactc aataagatgg ctgaaacgag aatgaagttg tacctgataa
     1981 aagaatgacg catgcctgtc tcctcatttt tgatttatgg ttttggtttt tacgttacaa
     2041 ttggtttgtt gatcattgat acggagaggt gtatgaaaga gccaattata ttgaaatctg
     2101 atgacccgat cttgaatacc ataaagttta aaccttatcg taacatggtt caacgacggg
     2161 ttgtgccttt tgtgccaggt gagggtcagc cgcagaccat ggaggtgatt acaccgtggg
     2221 gggcgcgttt ggtggctaaa aaaggtgatc tgttggttgg agagttgaat gccccggatg
     2281 atatgtggcc agtaagcccc gagatctttg atgcttcgta catggtcatc agcccgggga
     2341 tctgtgtcaa gcgggctgtg accttgctgg cgccgttgac cgacctgact gacggcgatg
     2401 aagaccagac ggttatcatt cacacccttg aaggggtgga tactgttcgt gcaggagatt
     2461 ttctactggc aaaaggggtc aaaggggaga tctggcctta ccccaaggcg aaagccgctg
     2521 aaattatgag acccgccgag tgatcggcgg gtcttttttt gaagtgggat tgggggctat
     2581 tttttcgaat cggagagctt gcgcactttg gtcgcattcg attttgcttt gccgctttcc
     2641 tgctcatcca tcaggcgccc gaggacattg tcgatcacgc tgtgcaggta ttttgaggtg
     2701 ttgatctgtt cgatggaacc gcctgtgtcg cgcatcaatt cgatcatgtg atggacaagc
     2761 cgcgcatcga ggtctttggg tttgtcaccg tgggcattcc acaaggcaat gaggtagact
     2821 ttgtcgatgc cttttccgag ggaggagtac aaagcccagc ggttgttacg ctcatagggg
     2881 tcatcgcctt cttttggcag gccaacgtgc tcgatctggt acatttcatc cacaagcggg
     2941 tgattacgga tcttgtggaa acggtccacc caattatcgc cagctgggga aacaaatttg
     3001 cgaatgtagg ctgattctgg caacggcagg aaagccttga ccttgatgcc tttttcggcg
     3061 catacttcgg caaagagaat ttcgcttcca gcagagagtc cgcctacgaa ggcgcggtca
     3121 tctggagtga tcttgaattt ctccagtttt ctgatgattt cctgacggat ttcattttcg
     3181 cgctcgggtg ggaaggtctt tttctctttg gcggggtaat ccaccatgta gccggtgaac
     3241 agaaaggcac gtgcatcggt cttggtcttt ttcgatctgc cttttttgct tgtgctgtga
     3301 gaagcatcat cgccgctggt gcgcatgatc tcgtctttga gagtgtcaat gcctacttgc
     3361 acgaactctt cacgcaggcc aagcgcttta aggatttcca attgcgcaag ggaggaattc
     3421 aggaagaaga gatttcggcg tgacgctgtg atggctttgc ggtaggcgcg tgccactgca
     3481 gacacgttgt ccgcggtcag cactcttaat tcggccagtg aaatcagggt ccagtaatct
     3541 gccctgtcat catcggttat gttttcgagg ccaaagatca aagagccgcg taattcgggg
     3601 aggttttggc ggatgcgggt gatatcgggg tcgggatctt tggggtcatc aaatttgtcg
     3661 gcgagatgaa cggcaatgat ggatagcgtg aatgcattga taccggggta gttgttgttg
     3721 aggtcaatgt gataccctct caggtaaata tccacagctc gaatcagcca gtgataggtc
     3781 tcaaaggcat tttttatgcg tttgttcttg tcgttgatgt tcttccacga accgacccac
     3841 atctccttgt agattcgacc cagataggca atcgcttcac tgtcattggg gaagtcattc
     3901 agcaggtgtt cgatcttgac aatggcttca tctacgcggc ccatgcggtt gaggttgaag
     3961 gcttcctgac gtcggaaatt caggttgctg gaattgactt ccaatccttt gcggtattgg
     4021 gcaagggcga gttcatttcg tcccatgtct gaaagggccc tgcctgcttc gccaatggcc
     4081 tcttctttaa tgagcgggtt ttggatttct tccgtgagca ggagaatatc gccgatgcgc
     4141 ttttggcgct gggcaaccgt gaccttgttt cgccattcgt tgtattcacg ccagaaacct
     4201 gtggcgagcg gggtgcgcag ggcagtgcgc tctggttctc gtaacccggt caaaagatta
     4261 aagatcgggc tgtgaatggc atcgcggtct gaggcccagg tatccctggt catgcgtgca
     4321 atggctttga tatctgcttt gatgtattgg ggatctggca cgccttcagg ggtgatgtga
     4381 tatggcaggg tgcggacgtt gaaaatatcg aaaggcatgt aggcacgccc agcctgaatg
     4441 tgtaccacgc cgcgttttcg aaatgcatga cggatgccaa gctcgtaata gacattggca
     4501 ttgtcaatgc ttaggtcaca cagacacagg tcggcgagca gcaattcctg gaacatgtcg
     4561 gtcagaatgt cgccggagga ggtttcttcg tcggcgcgga aaggctcaaa gccagcttct
     4621 tccagggctg gtttgatcaa ttccttgtaa attgcattga agtcgtataa cgaaccgtcg
     4681 ccaccttttt tcttgccgaa cggcatgacc acaaaagcat gcggtcggac ttcgggccgc
     4741 actggatgcg aagattcgct tgagcgcagc tcagaaagcg tggaagattt ttcgggttct
     4801 ttttcttttt ttgcggtttg ctcagccgta gtagatgcga cgatgggtgt ggcagcaacc
     4861 gctggggcgg gagtagttgt tgcggtccct gcctcattac ccgcttgctt ttgggcttct
     4921 ttaccagctt ctgcggcagg tgtggattga gccgtggggg tgggcgtggt ttcggtcatt
     4981 gttgatcctt gggcgttagc cctttacttc ggtcgtcttc ggtaaacgtg acatgaggtc
     5041 gttgagcacg ctcattgggg tgttggcgtc aatgtctaca ccggcaaatt catcatgaat
     5101 tgcttgcatc gcggaggaaa gcccgatttt ttcggagaag ttttcaaccg cattttgggc
     5161 ggcttcgcgc atggcggcaa gttcggcttg aacgaggtct gaggaggctt cttctgcgag
     5221 ggttccaagt gtggatttat aggtctcaac gagggtctct tcggtttcct gcatactctc
     5281 acgggtatga tccaccaccg cgtcagcgat cgcgcctcta aagcgtttgt ctgattcgcg
     5341 ctgttccttg atcacgcgat tgatgaggct ggcttcctct tcaaggtcag agtcgcgcaa
     5401 ggcttcctgc atcgccttga tgtattcgac attttgcgac cagaggatgt cttcggtgga
     5461 gcggacggtc ttgtagaatt cggtctggtc tcgttcttcg ccttcgaggt ttttccagtg
     5521 gtcgaagagg atgttcagct caagcacaac tttgctgtaa ttgcggacaa ccgaatcaag
     5581 gctgcgtaac tcctgccagc cgatgaaggc ggccgagaag gcggcggcca gcgccaccca
     5641 catggtgagt gaggagccaa tggcggcaaa caatgcgcca gcaaggccag ccagcagaat
     5701 gtaaatcttt aggcgggttc gttcagtttg ccttttgttg atttctttga tatgccagct
     5761 caattgatta tcaagcctgt agttgaagta ttcatctcca ttcaagtcgt tgaaaccagg
     5821 gtcgctgttt gggtatttgg ggtcgaaacg cggaggagga ggaagcattc ccttgtaggg
     5881 tttgagaatc atttcgccat tcatgccgcg atacactgag cgttgaattt cacccaggcg
     5941 gttttccagc caggtgcgac gggtgggtgt gcctttcaaa atggtgcggt aggcgtagat
     6001 ttctttgaga atctcttccg caccagcacg ggcaaccagc caatcacctg tggaatagaa
     6061 ctgactgaca tatgcagcca gtgcagaagc cgcaagtgga gtgacgatga agatgatctt
     6121 caagatcaga gctgccaccg ggttgagcct ttctgcaaag agctgggtga gaatggcaaa
     6181 aaaggtggtg aggacaccca gtgtagcgat ccatcttcgc aggcggaggt gggcctttga
     6241 tcgctcagtt gccatggcgt ccagttgcgc gaagcgggtc caggcgactt ccagaattgg
     6301 ggtgttttgc tgaggttgat tttccatact ggcttctcca ataggtctag ttttcaactg
     6361 ccaggttagg gcagatgggt cgatattctt catcagagaa caggagttcc catttgctct
     6421 ggtcgaaatt atgcgtgagg cgggagcagg cgttttcgat caggtctttg ctcaagatct
     6481 gtggaatgga tgaaactccc caaaacaaga cggttttgcg cgctgctgtg gatatggcgc
     6541 tgccgtctgg tgagaaggaa atgctggtta ccttatctac atgggggagg cgggcgatct
     6601 cttcgtgcag cccaatatca aaaatataaa cgaacccggt gctgtcaccc acagcaagcc
     6661 attgatcatc aggtctgaaa tcgagggaaa gcacttcgcc gttggccaaa aagccatcgt
     6721 tcaccggggc aaaagagccg tcgcctgcgt tccacaggga aatggaggtt tctgaactgc
     6781 ctgtggccaa caggtttccg ttgttgctga attgaacaga tttgatcttg ccgatctgtt
     6841 ccagtacttt aacctgggtc atcgtggtca gatcccagac aagactatgg ttggcgtcct
     6901 caatgcctgc caccagatat ttgccatttg gtgaaaccgc cagggtcaaa ataatcccat
     6961 ccgctgacaa tgaattcacc ttttcgccag tttccacatt ccaaatgtgg atgacgcctt
     7021 tttcatcggc tgtgatgatc tgtttgctgt ctggtgtgaa tgcagtagaa gtgatgactt
     7081 gtgtatcgtg agaaaggaag aactcgttgc tgccatctgc acgggctaaa acgacacggt
     7141 tgtaatcggc gatgttctcg tcgttttcaa ccgccgcaac ccagtttgag tcggcgctga
     7201 tcgccatatc gctggtcaga ccatcggttt cggcaattac cttgcgctcg ccttgtgggt
     7261 ttcctgattc gtccaggtcg atgcgccaga ttttgcggtc atcggtgttg acggccagcc
     7321 acttcccatc tggagagaaa tgcgcttcgt ttacaaactc ggaaaactgc tgaaatcctc
     7381 ggcgggcttt gagttccgaa atattccaca gggtcacatg tccatcgtaa tccccaacga
     7441 tcaagcgggt tgcatccctg ttgaagcgga tggaagaacc gatcccctcc aacggaatct
     7501 gcatggtctc tgctccggag gcggcgtccc agatgcgcag ggttttgtca taccctgtgg
     7561 tggcgatcca ttgaccgtct ccactgacac gcaccttttg gacaaaatta ttgtggacca
     7621 tgcgcaatct ttcctgacct gtggctgtgt ctataactct gacagtgtta tcgtctgaag
     7681 cagtgacaaa ccacgaacta tcggggccga aggtgacatc ttccacccag tcgccgtgcg
     7741 taacggaata tttttgtccg cctgccctgg attgcgcgct gcgggcagtg ctatcctgtc
     7801 cgacactgac tacccaattt ccatcaggac tgagttcaac atcaatgact tcgccattgt
     7861 gctttggtcc gccgaccgat tggttgccca tgaccgccca aacggttaca tttcctttgg
     7921 atgtacccat ggccataaag gaacctgtat catcaaaatc tatggtcttg acttcaccgc
     7981 ttagcgtgat aaacaacgct tcggtcaaat cggtcatgtc gatcatgctg atggcattgc
     8041 tgcggccaac accaagccag cggccgcccg ggtgaggatt gagatcccag atggtgcctt
     8101 catattgtat ggatttgatc tgctcgccgg tgtctacatt ccaaaaggtc agaacacctt
     8161 tttcggtgcc tgttgccagg atggttccat cctggctgaa atttgcatcg taaacaattc
     8221 cgtcatgctg ggcgcaaaag aattgagatc catcttccat gctccagaca caggcttttc
     8281 ctgccgaatc tgttgtgaca aatttggact ggtcagcact cggttgaata gcccagacac
     8341 gggcccccag gttcatttgt ttgaccggga tcggcagcag ggtgatattg ttgcgcagga
     8401 tatcctcagc gtcatccaac ccttccagtt tttggagagc attgaccgcc agcagggtgc
     8461 tggtatccaa ctcaccatcc ctgtcctgat atatttttgc ttctgctgcg ctgcgctgcg
     8521 cttccgcttt ttcttcgttt tcctgcgcaa ttaacttttg ttgttcagca atcacccgtt
     8581 gctcttctgc aatggcctgc tgggtggcac gtgccagttc atttgcaacc gcagtggaac
     8641 ggctgttgac cgcatccctt tcactttgcc tggcgatatt gcgctgtaga aatgcaaaga
     8701 tgccgaaaaa gatgctgatg accagcatgg ctccgacacc aatggtcaga ttccgctgac
     8761 gctgtaccga agtcttgcgg ctggtgctga tgtattccgc ctgcatcggg ttgacgctgc
     8821 ggttggggcc agtggtggaa gcggtcatcc attgttcgcc atcttccagg tcggtacctt
     8881 gcaggaggta gcttttgttt cgtcgtttct gatcccattc cgtggcgcgt tggagcaggc
     8941 gggtatgttg ctgtacccac tcgaggtctg tttgaatggc ttcgatcagt ttggggatgg
     9001 tgttcttgaa atcgtctttt ttcggccgta aatacaccca attggtggag gccagttttg
     9061 ggtgcatctt ttgttttttc tcaggctcac ggtatagaac gggaatgatc ttcttattgc
     9121 tggagatgcc gagttccaat tcatccatgc agaccttgga tttaagcgaa tcgggactga
     9181 tcacgaagac aaaggcatca ccaccttcga tcgcggctgt aattctggcc atccaatcgg
     9241 aagccagttc aatgccctcc cagtccaccc atgcgtcaat acccgctgca tcgagggctg
     9301 cgttcaattt tcgtacaaag agcttgtctt tgcgcgaata ggaaatgaaa atcttggttg
     9361 tccgcgaact ttcttccatg atggggtctg ctttgtatta gaaacttaca aaccaggccg
     9421 ctacatagcc ttcattggtc gcatctttga ccttgatcca ctggtcattt ttgccaacct
     9481 tggcttcgcc acctgcttcg gtaatggtga attcatagtt gatgggcacg cgcttgatca
     9541 aggtggcgtc gctcactaca ggctgattac gtagggagac catttcaacc gtggtcttga
     9601 ccttcttcga gcctgtcgct gcaggggctg atgtggccgt tgcttgtgca gtggaaccgc
     9661 ccgcatactt gacgtaccag gccgctacgt aaccttcctt cttatccgca ccgcgcacct
     9721 tcagccattg attttgtaca cccaccttcg gaatggccgt attggcagct tcaagacaaa
     9781 tcaactgttc tgtgactgga acccttcgga ccagagtctc gggtgaaatc acaggttgag
     9841 aacggaagga taattcttca gtggggtata gagccagcgc accagcagga acaggggctg
     9901 gggcggcagc aacgggtgct ggagcagcag caggtgtcga ggtggtcgtg gtcgaagcag
     9961 caggctttcc tttttgctca gaaacgaacc atgcggccac atatccctga tcgcccttcg
    10021 gatcctgtac ctggatccac tgaccttgta caccgatctt cgatttggcc tttgccttgt
    10081 cttccaggca gatcaactcg gtaccgagca gcatacgctt ccaaagatag ccgcttacag
    10141 aaggttcaga gcgcagggca agatcatcct cacctgcgta aaggagatac ttctccgcag
    10201 gtacgggaac cttggtcact tttggtggtt caggtggtgt gctgctgtat gagtcaaacc
    10261 acaacaccgc actgatctct gaggtgagtt ttgcaccatt gtatttgtat cgggtggtga
    10321 gcagcacttc cttgtcgggg ccgtcaccgc tgtacttata ggggtcgtat aaaacgtaat
    10381 cgtccccttt cttttccttc accaaaacaa aatgcgtttg aataccagcc tgcttgttcc
    10441 aatccacctg taaaatgacg ggcttgcccg ccgccaccgc cgcatcgatc tgcggaatgg
    10501 gtgcagggta agcctcacag ggctgcatgt cgcgataggc gcaattcggc cagacgtagg
    10561 ggaggtaact cggcataaat aaagccccgc taaaaccacc tgcagctttg aacttctcat
    10621 tggcggttac cggggtctca ttgtagccaa tcccattgag catcatggtg acggatgtga
    10681 gcaaacaacc ccatccaccg atggtttcac tggaatttcc gagaggagta gacttccacg
    10741 tttcgtcgtt ctggtagaga ttcttggtaa cgaacatcgg cttacctcct gcatttgtta
    10801 agttgtgaaa tttgattatc ggcaatttta ctccatttcg attcgcaatg aagttgtaat
    10861 tttgttaggt ttattgtgga aaaaaaagcg cttcaccccc aaaccgatgc ctgacagccc
    10921 atcatgggca tttttctccc cgtatgattt ccccgtaaat aatgagctta tttcgttgtt
    10981 gcgtactgcc taaaccacca ctctgcactg ggaatgccgc tggcctgtac agcctccatc
    11041 aaaaccgttg tgtcgaaata caccctgcgc aggtccacat ccagtgaatt cccgttctgg
    11101 ctgatgatcg tatattccgc ccaaggcagc agacgcggcg cattgcccgg agtaaatgcg
    11161 aacatgaacg aatttccgac actgcccgaa tttagaatca gtttatcccc atggcggcga
    11221 tacatttgaa tatgagagtg tcccccgata aacacggtcg cctggtggcc ttcaaagtat
    11281 ttatccagtg tctcgggtgg ggtggttgcc tgaatgatat cggtgggcga aagcggtgac
    11341 ccatgaaagc acaaaatatt gaccccattc ggcatgctga tttcgtgaag cgttttgaaa
    11401 gaggcaataa agtcaaggtc gtttttggtc aaacgttccc tgcaccaata caagtcgggg
    11461 atgagatggc tggtaatttc aaatttttca gcttcttcag ggtccaaaat agcggagtca
    11521 tgattcccct tgataatgac acagttcaat tcacgcaagc ggtcaagcac ttccctgggt
    11581 tgcggcccaa ggctgaccgt atcaccaaga caaatgacct catcgatgtt ctgtaccttg
    11641 atgtcatcaa gaaccgcttg taaagcggtg aagtttccgt gaatgtctga aataaaagca
    11701 atgcgcatcg ctgacctttt ggctattgta cacgaacaag agataatcct taccattttg
    11761 agctgaaata gaacttttgg gtgatggatt agattgagga tattttgccg ccaaaaatgg
    11821 ctgaaaaggg attttgttgt gaaattgttg cggttgtaaa tttgccgaaa ttgcttgtga
    11881 gaattgtatg ttgacctcca tccacctcct gtgccataat tgcgggaaat ttgttccgcc
    11941 aaacataaaa aacatcgact ggagaaagca tggcgaacca aaaatggtgc gttattaata
    12001 aggccaccct gtgtgaatcc ccaaagggtg ccaccgtgac tgaaatatat cttggggcat
    12061 tagttgaagt gacaggacga caggaagacg ttcccatgct cggcaaggat gggcaaacct
    12121 acacctccac atgggtggaa tgtgtttacc gtaactcaca aggcaccgtt gctggttggg
    12181 tgcgtgatga attcttcgat gactacgtgg agaaatttcc tccacccgtg gttgaaattc
    12241 ccgcggccac ggaagagcag atgggcgaat ttccctatgc caccaaaaac cccaacgacc
    12301 ctgcccaata tctggtatta ggcaggaaca gcaaaggcga agagcaggtc aaatacaacc
    12361 tgtgcgccga attttgtgtc gcttttgtca tgggggttgg gatcaaacaa ttcctgcgag
    12421 attgggagaa cagtgccggc agtttgtata agtgggcact gggcggtgac agtgacaaaa
    12481 cctccgacct ctccctcgtc aaaaacatgc tgaatcccta ctcttacagc gcggcgaacg
    12541 gaaatatcat tgactttgca gttacgttga tcggccccgc cttcgaagac aaaactttga
    12601 gccctggccg cttccagaaa atgttgcaaa cacattccct gctggctcag gtggtcatca
    12661 acaaaaatac aggcaagttg atccccaaca acgagaccaa acgcgcgaat ggcggtatta
    12721 atcattggat cgttttggaa agcgccacac caaacggcac agatggcggg cgtgtggaaa
    12781 tatacaaccc gttcaataat cagcgccagg aatatagtta cgaatatttc attagttcct
    12841 ttgggggcgg cacatacacg gggtgctggg tcaaacgcag gtcagagatg gtggccgtca
    12901 aagcaatgga tgaagttgcg tcggccaaga aatggtgcat tgcccccggt actttccgcc
    12961 aaacccccga tggtgttggg gtgttgagtt ttatgaaggg cgcagtgatg gaatcttcag
    13021 gcgccaccga agtcaaaaat ggcaagacct ggatacaggt caaatacaag agccgaacgg
    13081 gttgggtgcg aaattccgtt cttgaagatt acgaagaccg tttctcaact cccgaagtca
    13141 tcatcccgca tcctacaccg gaggaagatg atgctgaaca atatatgttc ctgcctggtg
    13201 aagacggcaa aaaacgcaat atgtgcggac agttgtgcgc ctctttcatt atgaatatcg
    13261 atgttgaaag ttttgtgcag gattggaagc tcaaggccag gccgtattat caggtgtcca
    13321 ttgcgggaaa gaatgataat ggcactggca ttgattcact ggagaccatg tttcgggctt
    13381 ctccctacaa tgcagccccc ggcgatgttt ttcgttttga attgggaatg aaagaccctg
    13441 tgaccggcat cctcattctt tctgcaggca ggatgaagaa aatgctcgaa acccactacc
    13501 tcgtagcggg tgtgcggatt cgacaaagcg acaagctgac aggccgattg agcggacagg
    13561 gcattgggca ctgggttgtg gtggataagg ttttccccaa tggcaaaaac agcggaaatg
    13621 gcggctgggt tgagatctac aatccctatc caaaccgccg tcaggaatat gcctacgatg
    13681 aattcatgca gtcatttgca ggcacgcttg gactgtgggt gcgtagaaaa taagcccttc
    13741 aattgctgga agggaaatca ccgcgccaca tttcaatgcg acggaacgat tccacatacc
    13801 ccattttgta ataagcaggc aggtgtggat cgttaacttg aatattttca atgtgagtat
    13861 ccaaacgcgg atactgggaa tgtaaatgtg tcaacagagc gtaggcgacc gtggctgggt
    13921 cgcctgcttc tgttttaatg gcaaaacggg taagtaggaa tttttcgcgt tgatatacca
    13981 gccagccgcg actgccatcc accagcgtga tactcagacc cgaaacctca tgggcattga
    14041 tcaaggattc ggattggttg gtccagggtt gggtgccgcg gtcgtgaccg accagcacca
    14101 gggcttcagt acgatccatg cgttgggcat ctgccacgat cgggtcaatg ggtggaatca
    14161 gcggggaacg ccgcaagacc aaaagctcac cgatctcgcg gaagccatat ttcaaaaaaa
    14221 gctggtgtgc gggtgtgttg ttcttgatca cttcgagcat gacaaaatcg aggccaagtt
    14281 ttgctgcatc ttcgatcaaa ccgcccacca atgcgctgcc gattccactg cggcgcgtgt
    14341 tgggcagaac cccaaggcgt gtgagccaga cgcgtttttt tcgaatgccc agcatggcaa
    14401 ccccgagcat ctgcccatca tgggttgccg ccaccagtga atgatccagg tctacatcgt
    14461 agatgcgcac gtactctgcg aggcgcgccg cattcatcgg catggggatc atgtagtcga
    14521 cgcgtgtttg gttgtagatg gctgtcagtt cttcataatt gaaacggctt gcagggatta
    14581 attgaatggg ttcgttcgag tcaggggttt gcatttattg ggctttgaga aatgaattga
    14641 ttctggcgat gaaatcggca acccgggtaa tgggtttgct gatgtaacca ctgaaaccat
    14701 aactttccac catctctttt gcattttcct cgggccaggc tgagagtgca acgatggggg
    14761 tgttggccag ttcgggggtt tcgcgcagcc accccgccag cgtttgacca tcatgatcgg
    14821 gcagaccaag atcgagcaga atcagatcgg ggatggtatc cagcgcagat tgcaggccag
    14881 tttctgcatc gggtgcgtgg atgatctgat ggtcggtgcc agagagggct cgccgtacaa
    14941 gttcaaaatt atcctgaata tcttcaacga ccagtatctt ggccatgctt cacgctcgct
    15001 tttctttgat cgcataccgg acagggttcc gtcggttgac tatagcatag ccaaccgacg
    15061 gtggttgagc aagtggagaa tgtaataaga aacggcatat tttctatttg ctctttggtt
    15121 tatcttattt tacgccactc caaaaattga acggtggcaa acagatcaac gcaaatggct
    15181 gtgatgccaa tggcccattt ggcttctgcg ctgaagttga aagcagggag tatcaataaa
    15241 agcacactga acagcaccca gagcaaatct gcaccgataa cgaatatgct gaagaaatta
    15301 gtgatcaaac ggcgtgtagc catgtaaatc acccataccc cgtatgcaat caatccaatg
    15361 cccaaaacgc tcatcacaac tggaacggta ttcaagaatc cagatagagg tttggcggcg
    15421 agcaggaaga tcaatccact ggtggagcag aagacaccat tggccaataa gatgcggctg
    15481 aggttgagtt gattgttcat tgtttatgcc cttctgaaaa tttggtaggt tgcagaaagt
    15541 ggaatgacaa aaaggattgc aaacactgta ttgagtacgg cattctgcca aaagcccgtt
    15601 gcaatcgaca tggcggtgtc agggatgaac cagaggatga gtgccaccac gaggctgagc
    15661 catccatgcc attccccttt gcggaattgg gtgaagagtg tgtacatcat tgatacaccc
    15721 caaccgatca tgacggcgcc catcactgca tgggtgaggg tgatgtaacg gattgcgtcg
    15781 agtggaaatg aatctatata agaggctgag ccgaagacca tcaaactaaa gccctgacgc
    15841 accagattgg gcaagaggac ctgtgcgagc ccaaaaagaa tcacgccgat gttggcagtt
    15901 accaaccagc gccaccaaaa attgagattg ttttttgtat tcatatgagt ttctcctttg
    15961 cccccagtgt acgaatcgcg cactgccgag tccacgaaat gaactgccat gtactgccag
    16021 ttattaaatt gacttgtata atccgaactt tgaaattcaa attccgccaa ggaggatgag
    16081 atgagaatcg taaatgggag agttgcattg gtttttgctt tggcagcttt gttcttgctg
    16141 acaggctgcg ggggcggaga aaaatatacc tatcgtgtca acggtttggc tgggcaggca
    16201 catgtggaat atgccagccc cgatggaagc atggtggaag aggatgttca attgccatgg
    16261 agtattgaag tggatctggg caattccgcc gaattcactt tgta
//
LOCUS       DAOQJC010000091        16100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1723_length_16100_cov_8.200187, whole genome shotgun sequence.
ACCESSION   DAOQJC010000091 DAOQJC010000000
VERSION     DAOQJC010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 16100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16100
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1723_length_16100_cov_8.200187"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PLE39_13430"
     CDS             <1..564
                     /locus_tag="PLE39_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69380.1"
                     /translation="IRKRIEIHLAADKPSVRLKHTLINDGLWPVELAPWAITQFRLGG
                     TVILPMPVGNVDAAGLLPNRQISFWPYTRINDPRLNLRDDYVIFKADALPPFKMGYFN
                     PHGWLAYWLDGVLFRKTFEVQTGSLHPDNNCNAEIYCGDQFVELESLAPLEKLDTNAS
                     VTHTETWDIFTDLDSLPKEAQQLLAAS"
     gene            661..1821
                     /locus_tag="PLE39_13435"
     CDS             661..1821
                     /locus_tag="PLE39_13435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE69381.1"
                     /translation="MQTTTSKNTILILTAGCFLSFFVFGFTDNLKGPTLPAMIAELNI
                     NYGTSGNIFFGQYLGFLITSFIAGVLADRFGLKTVLIIAGAFLFIGVTGYSSFQSAVL
                     LGVSLFLIGLGLGAIELGANAAIVEAHPEKRGLYLNLMAVMHGLGSLLAPLFAGWFLS
                     MNTNWRTIYRWDIALIALFIFLFSFVRFPKSKEHNSIDFKHIAQIAFKGNLPWFYFAI
                     ACYVAAEIGLSSWLVTFLQELRSASVTSSTQALSLFFTMLMLGRLGGGFIVQRLGYLR
                     AILFASVGALVCLAAGTFTNFSVLLPITGLFFSIIFPTFTAAVSIDHPESTNSILGIL
                     FTFAGLGGLIGPWAIAWASDLMGLQLGFSVGIIFTALLLWAIFVLEKGKRNG"
     gene            1814..2794
                     /locus_tag="PLE39_13440"
     CDS             1814..2794
                     /locus_tag="PLE39_13440"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNE69382.1"
                     /translation="MDRILNWGLLSTAKINRALIKPLNASKRTRLLAVASRNISSAEA
                     YAREWSIPRAHGSYEALLNDPEIDIIYNSLPNHLHAEWTIKALRAGKHVLCEKPLALS
                     LEEVDAMIAASKETGKVVTEAFMYLHHEQTARIKQIVDNGELGTLQLIKGAFSFTLSR
                     EGNYRWMTEMGGGSIWDVGCYPISFARTLVGAEPVEVFGWQVTGQGGSDDAFVGQMKF
                     EGGVHVQFDSSFRSPHRSYMEIVGSEAVLKISNPFKPGKDNNLILVRGDDETVIRVKG
                     GELYMGEVDDMCDAVLNGKPPHVSLEASRNNTAVILALLESAKSGKLVIV"
     gene            2849..3949
                     /gene="ugpC"
                     /locus_tag="PLE39_13445"
     CDS             2849..3949
                     /gene="ugpC"
                     /locus_tag="PLE39_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019292901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNE69383.1"
                     /translation="MASVSFQKVHKKFGDLTIINSLDIEVQDKEFLVLVGPSGCGKTT
                     ALRLLAGLEEITGGEIKIGDRLVNDVAPKDRDIAMVFQSYALYPHLSVYDNMAFGLKL
                     RKTPKEEIKRRVNEAADILGIQDLLQRKPRQLSGGQRQRVAVGRAIVREPKVFLFDEP
                     LSNLDAKLRVQMRAEISKLHQRLQTTFIYVTHDQTEAMTMASRIAVINKGDLQQLDTP
                     QNLYDRPNNLFVAGFIGSPSMNFFPAKIVKEGGKVMVDTEDFKVQIPENLAAPYKNLE
                     GKNVVFGIRPENIHDPEFAPNNIHGEKVSSKVDVTELMGNETLLYLVSGKNTYVGRVD
                     PRSKLRVGNQSQFIFDMDKFHIFDAETEKAIR"
     gene            4235..5395
                     /locus_tag="PLE39_13450"
     CDS             4235..5395
                     /locus_tag="PLE39_13450"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNE69384.1"
                     /translation="MGLTRAAVTLIVNDLIENEVIFEAESRSIPSGRPPVVLEINPKR
                     GLVAAVDMGATHLNLAIADFSAKIIGEKSLPLDIRKGPQACMPEVISNLKALLSEYGV
                     SSPQLMAIGIGVPGPVITDAGMVVSPPIMPGWDGYPIRQTLEQEFGCAVSLNNDAELG
                     ALGEWAYGAGRGEKNLAFIKVGSGIGAGLIINQQIYGGTTGSAGEIGHITIDENGPLC
                     TCGSRGCLEAFAGGHAIALQAKKLVESGKRTLLSSIPVENLTAQEVAKAARRGDLPSQ
                     EIIMRAGTFIGIAIAGLVNLFNPGAVIIGGGVAQSGDLLTTSIRQAVRERSLRASERG
                     VHITTAMLGRRSSLMGATVQAVNIAIHDAIEKKNPVARDSFPSEIKQVFESN"
     gene            5500..6819
                     /locus_tag="PLE39_13455"
     CDS             5500..6819
                     /locus_tag="PLE39_13455"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNE69385.1"
                     /translation="MKTKLSLLFSLLVVASMVLTACGGGAVATEAPAVEEPAATEAPA
                     TEAPAATEAPAAEPITITFWEQEGEDVDVFIDGLIADFQAANPNITVERTHYENEALR
                     DQFQTASLAEAAPDVVRVPNDFAGPFSTLDIIAPVDALYDDAFLGQFFPGALDPAKVG
                     GTLWGVPDNYGNHLMLIYNKSLIAEPPATFEELITVSKELTAGDVQGFAYNLNEPFWG
                     AGFYGAFGGWPLDESDQPQFNNQAFIDYLTFVASLKTDGVVPAECDYACADTLMKEGK
                     AAMIINGDWSLGDYTTALGENLGVAPVPTINGNPYTEMTAGKYFMVSKAVLDDAARQE
                     AVTAFITYMTSAEVQKKWLDEFKRLPSNAEVAKDPSIASDPILAGSMADLAVGRGQPA
                     APEMRCAWDAWRPNLEGVMAGTVTPEDAAVAAQEAADACVATLGTTP"
     gene            6982..8484
                     /locus_tag="PLE39_13460"
     CDS             6982..8484
                     /locus_tag="PLE39_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNE69386.1"
                     /translation="MESDNFLRRNLPNILWIAVIVSIYFVLSSLIAPFSDGITLSSKI
                     VGERLQEIGSFLFAILGGLAIIEIYFYQIFFRQPLNRTATIITRAIQVAMGLLTFTFI
                     IYFFNLTDLIKLGDSAIGKFVNMPVDSFANLQKSLISSKVATKDSAKMVAGIIFALGI
                     LVLYAIIQFTSKPRNSRVGLAYLLIMPAFIGVLFLIIYPFLFEIKLAFSNASLGTQAT
                     KNAQYSLLFGWENLKTLFTGTVAKDAKFFEVFWRTILWTVINVTFHVLGGMGLAILLN
                     RPLKFKGLYRTLLVIPWAIPTTIAAMALRSEFDSRYGLFNILLGNLNTWLTTVSQNTA
                     NIIGIGNGFAWLAATIGPVSWKQDPFWAFVSVLIANIWLGIPFMSVIILGGLQSISQE
                     YYEAAEIDGATRWQQFRNITLPLLRPVLTPAIILGVVWTFNKFDIIYLITQGGPQEKT
                     DILVSSMYKAAFQFYRYGFTAMFALVIFAILFIFTLFYLRVSDGLKSATE"
     gene            8495..9397
                     /locus_tag="PLE39_13465"
     CDS             8495..9397
                     /locus_tag="PLE39_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNE69387.1"
                     /translation="MTTNPISRFFRWFFYRSRGDSPFKTILIHATLIIACVIAVYPVL
                     RVVTISLRPNDTLLTTDLRIIPENATLDNYREVLFGDAEKNRQSDFFLWIWNSVSVVA
                     VTSVISVILAAISAYAFSRFRFPGRSAGLIFLLTTQMIPAGMLLLPLFIMIAKLKMIN
                     TPLGLIIAYAVTSVPLTIWTLKGYYDTIPVDLEEAALIDGASRLTVFTKIILPLSTPA
                     LAIAFLFSFMGGWSEYLVARVVLQKNEMFTWPLGIFTFAQQFTVSWGQFAAASVLIAI
                     PIMALFLYSSKWLISGLTLGSVKG"
     gene            9466..11082
                     /locus_tag="PLE39_13470"
     CDS             9466..11082
                     /locus_tag="PLE39_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNE69388.1"
                     /translation="MTKRIFLSLLLFLLISSCAPATPTTAPAPTTVAPPKIWWSDAVF
                     YEIFVRSFNDSNGDGAGDFNGVTQKLDYLQELGITALWLMPIFPSPSYHGYDVTDYYG
                     VNPQYGTMDDFKNLVAEAHKRDIRIIIDLVINHTSDKHPWFREAKKDPQSPYRDWYIW
                     NETDLKYEGPWGQRVWHSSTTGFYYGIFEAFMPDLNYANPAVTEEMKKIAAFWLADVG
                     VDGFRLDAAKHLIEDGMKQENTNATHLWYQNEFYPAYKAVDPEAMTVGELFGDGLNTI
                     ANYIKNDQFDLAFNFQLAASFLEAVNTEKSGDLARTLQVSDKAIPDHQYATFLTNHDQ
                     NRVMTQLGGDVNKAKLAAFLMLTSPGTPFIYYGEEIGMQGKKPDENIRLPMQWTSGEN
                     AGFSITRPWRAPASDYVFINVDAQTGAIDSLLEHYRALIQLRNANPAMQTGNLISLKS
                     SHPSVYSALRINENGMFLILANLSKETISKYNINLNGAGLAESAYSVETMFGAGQAAG
                     PVGSGEAFQEYKPFENLDPYAMYVLKLISK"
     gene            11095..12564
                     /locus_tag="PLE39_13475"
     CDS             11095..12564
                     /locus_tag="PLE39_13475"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNE69389.1"
                     /translation="MSTPEWVKDAVFYQIFPDRFAKSSRNPAAALPFEPWDSAPTTHG
                     FKGGDLYGVLEKLDYIQELGCTAIYLNPIFASASNHRYHTFDYYNVDPLLGGNEALKK
                     LLKEMHKRGMRLILDGVFNHASRGFWQFHHVLETGDSSPYKDWFHFDHDRLTGKKHWG
                     AYPTPHEDKILQHEDSLTAIGYRAWWNIPALPKFNTKTPAVREFLFEVAEYWIKFGID
                     GWRLDVPGEIDDDEFWREFRRRVRAINPDAYIVGEIWHEAQRWLQGDQFDAVMNYLFT
                     SASLSFFAGQHLDMGVISQAGGIKDRVRAVNAYEFANEIDRILNLYPPDITSAQLNLL
                     DSHDMPRFLSCASGDKAALKMAWLLMFALPGAPCIYYGDEVGVDGRHDPECRKAFPWD
                     ESKWDKELLEFAKACTTLRTEHAALRRGEYKRLYAEGEVAAFMRSYKNEHLTVVFNTA
                     TEDRSIELHFEKKPRVLLGNASLDGNRVTIPARSGIVLK"
     gene            12887..16096
                     /locus_tag="PLE39_13480"
     CDS             12887..16096
                     /locus_tag="PLE39_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69390.1"
                     /translation="MTENEAPTPAEILKSILEGLSGADASVRLEAIEKLHSINYSSEA
                     IYIELERLAIHDDLEEVRKRALEALDLATHKHVRGRVNRLNAGDRTVLVEEIGEWESL
                     GLLDLTRAAVLRRRYDFDKRAKVTADAVMSEKPATVEKAEPAPKAEPAPAEPRPTLMQ
                     TLLSETSIKIALYLGAFFVVASAAILAAVSPELRLFVLIVASLGFGGLSLGIRKRLPQ
                     PSFALFIVFSFLLPITANVLQDTLGLTDVVKAVYWVVISLGMAVLWGGSIWLYESRVF
                     SVMSFLAFVLAFYRIGEVFEAEGEVYGLLMGAATLTGLGLVWVLKKWRDDKFALPLFL
                     STLAVQALILGGTLVLFAIQLFDGNDSALWNLASMLTWGLAFIFLVLADVLFPFVFFA
                     WFAALALVPIPWFLGAAFDMEFNGLAILFFVWGLILSVFSEAGFRLEWTRKFSLPVAM
                     VAIPAFVLALINAFSEGVSLGFGIALGTTLVYAVLQLLRGREWVWALALLNFFIAYFA
                     FFNLTLIEPLKVYFGYQALGLSLLLMLPGLFGKNDLRTASTWKLPTLAFGVIFTLTAT
                     VAFVIGENSLHAMVGFGVLAVFFTAYALVQGSAFYGYLPASFLALSALFGVEYLDKDW
                     WLPVLTTLAVLYYACGAALLSRAGWSRMLRNSGLVLGTLIVVAAAAQMKGAGGWYAMV
                     VGLIFAVEMYLSGEGLLEIAPPIFFSAGVILVQRAIPTPMGGTHQYLVHSLVWLGWDL
                     LMHLTFKQARPLSWLVRGGGALVTLMNYLILMDVGSAKAGALDFGIYTLLFLTVNLVY
                     RRPILFHTFGVTLPLFVMFVFRLVGVEKWIHPVIFVAMGFYAVGFALRWMKLEWAKDW
                     DKALILEGLGWGMVISIAAPTLGGLDASIPVAFAATLWAAEAFALRDVRLGFPANGLY
                     LLAYFIILNELNVDQPQFYSMGAAALGMFQHYLLTRSGSKTGTFVMGMLSQLTLLGTT
                     YIQMVDSEKLIYFVVLFFQSIAVLVYGLVIRSRSLTFTPIVLVVLGVVTVLYTALKGL
                     NAVVLIGCTGVILLGLGIAAVLLRERIARLGERLSDWRA"
BASE COUNT         3671 a         4127 c         4184 g         4118 t
ORIGIN      
        1 attcgcaagc gcatcgaaat tcaccttgcg gctgataaac catctgtcag gctgaaacac
       61 acgcttatta acgatggact ttggcctgtg gaattagccc catgggcgat cactcaattc
      121 cgtctgggcg gcacggtgat cttacccatg ccggttggga acgtggacgc tgcggggctt
      181 ttacccaacc gacagatttc cttctggcct tacactcgca tcaatgatcc gcgcctcaac
      241 ctgagagacg actatgtcat tttcaaagct gatgcattgc cgcccttcaa aatgggttac
      301 ttcaatcccc acggctggct ggcctattgg ctggatggcg ttctcttccg caagacgttt
      361 gaagtccaaa caggatcgct gcaccccgac aataattgca atgcggaaat atattgcggt
      421 gaccaatttg tagagttgga aagccttgct ccacttgaaa aactggatac aaacgcatct
      481 gtaactcaca ccgaaacatg ggatatcttt accgatctag attcactgcc caaagaagca
      541 caacaactac tggcagcttc ataagcctgc tcccggtgaa atttcaaaac accgggagtt
      601 ttcttttccc agcctgacac agaaaaaagc ccagcttcaa ccagtataat ttcctccatc
      661 atgcaaacca caacctcaaa aaacacgatc ttaattctga cggctggatg cttcctctcc
      721 tttttcgtct tcggttttac cgacaatctc aaaggcccca ctctgcctgc aatgatcgca
      781 gaactgaaca tcaattacgg cacaagtgga aatattttct tcggccaata tctcggcttt
      841 ctgatcacat cctttattgc tggtgtactt gctgaccgtt tcgggttgaa aaccgtcctg
      901 atcatcgcgg gggcctttct tttcattggc gtcaccggat acagctcatt tcaaagcgca
      961 gttctgttgg gagtttcact tttcctcatc gggttgggct tgggcgccat cgagcttggt
     1021 gcaaacgccg ccattgtaga ggcacatcct gaaaaaagag gcctctacct aaatttaatg
     1081 gccgtcatgc acggactggg atctctgctt gcaccgcttt ttgctggttg gtttttatcc
     1141 atgaacacca actggcgcac gatttatcgc tgggacatcg ccctgatcgc ccttttcatc
     1201 ttcctttttt cttttgtgcg ctttccaaaa tcaaaggaac acaacagtat tgatttcaaa
     1261 cacattgccc agatcgcctt caaaggcaat ctcccctggt tctattttgc aatcgcctgc
     1321 tatgtcgccg ctgaaatcgg attatcctcc tggctggtca cctttctgca ggagttacgc
     1381 agtgcaagtg ttacatcaag cacgcaagcc ctttcattat ttttcaccat gctaatgctg
     1441 ggacggctcg gcggcggttt tattgttcaa cggcttgggt atctgcgggc gattttattt
     1501 gcatcggtcg gcgcattggt ctgcctggca gctggcacat tcacaaactt cagtgttctt
     1561 cttcccatca ccgggctttt tttctcgatc atcttcccaa ccttcaccgc ggcagtatcc
     1621 attgaccatc ctgaaagtac aaactcaatc ctgggcatcc tcttcacttt tgcagggttg
     1681 ggcgggttga tcggtccgtg ggccattgca tgggcaagcg acctgatggg gttgcaactc
     1741 ggtttttcgg tcggcatcat tttcacagcc ctcctgctct gggcaatctt tgttttagag
     1801 aaaggaaaac gaaatggata ggattcttaa ttggggattg ctctccaccg caaaaataaa
     1861 ccgcgccttg atcaaacctc tcaacgcatc aaaacggacc cgattgctgg ccgttgcttc
     1921 gagaaacatt tcctcagccg aagcatacgc tcgagaatgg agtattccgc gcgcgcacgg
     1981 cagttacgaa gccctgctca atgacccgga aattgacatc atttacaatt ccctgcccaa
     2041 tcacctgcac gctgaatgga cgatcaaggc gttgcgcgct ggcaaacatg tgctgtgtga
     2101 aaaacctctt gctctctcac tcgaagaagt ggatgccatg attgctgcct caaaagaaac
     2161 tggaaaagtg gtaaccgaag cattcatgta tcttcatcat gaacaaacgg ccaggatcaa
     2221 acagatcgta gacaatggcg aattgggcac cttgcagctt ataaaagggg ctttctcttt
     2281 cacactttcg cgtgagggca attaccgttg gatgaccgaa atgggaggtg ggagtatttg
     2341 ggacgtggga tgctatccga tctcttttgc ccgtaccctt gtcggcgctg agcctgttga
     2401 agtatttggc tggcaggtta caggtcaggg tggaagcgac gatgcttttg taggtcaaat
     2461 gaagtttgag ggtggagtcc atgtgcagtt cgattcgagt ttccggtcac cgcatcgttc
     2521 ttacatggaa atcgtcggtt cggaggcggt tttgaaaatt tccaacccat tcaaacccgg
     2581 taaggataac aacctgattc tagtcagagg cgatgacgaa acggtcattc gggtgaaggg
     2641 cggcgagtta tacatgggcg aagtggacga catgtgcgat gccgtgttga atggcaagcc
     2701 tcctcacgtt tcgctggagg ccagccgcaa caacaccgct gtgatcctgg ctctgctcga
     2761 atcagccaaa agcggaaaac tggttattgt ctaagttgtt ttgcgggttt ataattcagt
     2821 cactatattc ttttgactgg aggataccat ggcaagcgtt tcatttcaga aagtgcataa
     2881 gaagtttggt gatctgacga tcatcaacag cctcgatatc gaggttcagg acaaggaatt
     2941 ccttgtctta gtcggcccat caggttgcgg taaaaccacc gccctgcgtc tgctcgctgg
     3001 tttggaagaa atcaccggcg gcgaaatcaa gatcggtgat cgcctcgtga acgatgtggc
     3061 acccaaagat cgcgatattg ccatggtatt tcaatcctac gcgttgtacc cgcacctttc
     3121 agtgtacgac aatatggctt ttggtctcaa gcttcgcaag accccgaagg aagagatcaa
     3181 gcgtcgcgta aacgaagctg cagatatttt aggcattcaa gatctgcttc agcgcaagcc
     3241 gcgccagtta tccggcggtc agcgccagcg tgtggctgtg ggtcgtgcca tcgtccgcga
     3301 gccaaaggtc ttcctgttcg atgagccgct ctccaacctc gatgcaaaat tgcgcgtgca
     3361 gatgcgtgct gaaatcagca agctccatca acgcctgcaa actacattca tctacgtgac
     3421 ccacgatcaa acggaagcca tgaccatggc ttcacgcatc gccgtcatca acaaaggtga
     3481 tttgcaacag ttggatactc cgcaaaacct gtatgaccgc cccaacaacc tgtttgtagc
     3541 tggtttcatt ggctctccgt ccatgaactt cttccctgcc aagattgtca aagaaggcgg
     3601 caaagtaatg gtggataccg aggatttcaa ggttcaaatt cctgaaaacc ttgcggcccc
     3661 ctacaagaac ctggaaggca agaatgttgt cttcggcatt cgccctgaaa atattcatga
     3721 cccagaattc gccccgaata acatccatgg cgagaaagtt tcctccaagg tggatgtgac
     3781 cgaattgatg ggtaacgaaa ccctgcttta tctggtaagc ggaaagaaca cctatgttgg
     3841 ccgtgtggat ccccgctcga agctgcgcgt tggaaatcaa tcgcaattca tctttgatat
     3901 ggacaaattc catattttcg atgcagagac tgaaaaagcc attcgctaag aagtcaagaa
     3961 ataacataaa aaaggactgg agtttgtagc tccagtcctt tttagtttag ggaagaaatt
     4021 ttaagcatga attttgttaa aacacttgat ttaatactat tttgtgctat tatagaaaag
     4081 gtaattttga aaccaaaaaa gttttgtagc ctaaaaaatc atgttttatt ggatacccac
     4141 caatcaaaat attaagaatt ttaacaaaca ttctgcggta gatttgattc gattcgccct
     4201 caacggcatt tcaagaacag atcttgcaga acaaatgggt ttaacacgtg ccgcagtgac
     4261 tttgatcgta aatgatctga tcgaaaatga ggtcattttt gaggcagaaa gcagatccat
     4321 tccaagtgga cgccctccag tggtgctgga gatcaaccca aagcgtggct tggttgccgc
     4381 agtggatatg ggcgccaccc acctaaacct agcaatcgct gacttttcgg caaagattat
     4441 cggtgaaaaa tccttaccgc tggatattcg aaaaggccct caggcttgta tgcctgaagt
     4501 aatcagcaat ttgaaagcat tgttaagtga gtatggggtt tcctcacccc agttaatggc
     4561 catcggaatc ggtgttccgg gcccggtcat tacagatgca gggatggtgg tttctccacc
     4621 catcatgcca ggctgggacg gctacccgat ccgtcagacc cttgaacagg aatttggctg
     4681 tgcagtttct cttaacaacg atgcagaact gggtgctttg ggtgaatggg catatggagc
     4741 aggccgcggc gagaaaaatc tcgcttttat caaagtaggc agcggaattg gcgcaggcct
     4801 catcatcaat caacaaattt acggtgggac aaccggttct gctggtgaaa ttgggcatat
     4861 cacgatcgat gaaaatggtc cgctttgcac ctgtggtagc cgtggctgtt tggaggcgtt
     4921 tgctggcgga catgcgatcg ccctgcaagc taaaaaattg gtcgaatctg gaaaacgaac
     4981 cttgctttcc agtattcccg ttgaaaacct gactgcacag gaagtagcca aagccgcacg
     5041 gcgtggcgac ctgccttcgc aagagatcat tatgcgcgct ggaacgttca tcggcattgc
     5101 cattgcaggt ctggttaatt tgttcaaccc aggtgcagtc attatcggcg gcggtgtggc
     5161 acaatcggga gatttgctaa ccacatcgat ccgtcaggcg gtgcgggagc gttcactgcg
     5221 ggcctccgag cgcggggtcc atatcacaac cgccatgctt ggaagaaggt cgtcattaat
     5281 gggtgccacc gtgcaggcag tgaatattgc cattcacgat gcaatcgaaa agaaaaatcc
     5341 tgttgcgcga gactcattcc cgtctgaaat caaacaggta tttgaaagta actgaaaagg
     5401 aggtgatcgt ctgcactcag aaaaacaaac atgcacaaat catgaaatga tcgttcaata
     5461 ccaatcaaca taatcaccta ttaagtaagg agaagagtaa tgaaaaccaa attaagtttg
     5521 ttgttcagct tgttagtggt ggccagcatg gtcctcactg cctgcggtgg cggagctgtc
     5581 gcaactgaag cacccgccgt agaagaaccc gctgccactg aagcccccgc taccgaagct
     5641 cctgctgcta cagaagcccc cgctgccgaa cccatcacca tcactttctg ggaacaggaa
     5701 ggcgaagatg tagacgtgtt catcgacggt ttgatcgctg atttccaggc tgccaacccg
     5761 aacatcaccg ttgagcgcac tcactacgag aacgaagctc tccgcgacca gttccagacc
     5821 gcttccctcg ctgaagccgc ccccgatgtc gttcgcgttc caaacgattt cgctggtcct
     5881 ttcagcaccc tcgacatcat cgcccccgtt gatgcgttgt acgatgatgc cttcctcggc
     5941 cagttcttcc ccggcgctct tgatcctgcc aaagttggcg gcaccctttg gggcgtcccc
     6001 gacaactacg gcaatcacct catgctgatc tataacaaat ccttgatcgc tgagccccct
     6061 gccactttcg aagaactcat caccgtttcc aaggaactca ctgctggtga cgttcaaggt
     6121 ttcgcctaca acctcaatga gcctttctgg ggcgctggct tctatggcgc tttcggcgga
     6181 tggcctcttg atgagagcga tcagcctcag ttcaacaacc aggctttcat cgattatctg
     6241 accttcgtcg cctccctcaa aaccgacggc gttgtgcccg ctgaatgtga ttatgcctgc
     6301 gccgacaccc tgatgaagga aggcaaagct gccatgatca tcaatggcga ctggtctctc
     6361 ggcgattaca ccaccgctct cggtgaaaac ctcggtgttg cccccgtccc caccatcaat
     6421 ggcaacccct acaccgaaat gaccgctggc aaatacttca tggtctccaa ggccgtcctc
     6481 gatgacgccg cccgccagga agctgtcacc gcgttcatca cctacatgac ttccgctgaa
     6541 gttcagaaga agtggcttga tgaattcaag cgccttccct ccaacgccga agttgccaag
     6601 gatccgagca ttgcaagtga cccgatcctc gctggctcca tggccgacct cgccgttggc
     6661 cgcggtcagc ctgccgctcc tgaaatgcgc tgcgcatggg atgcatggcg cccgaacctc
     6721 gaaggcgtaa tggccggcac cgtcacccct gaagatgccg ccgtcgccgc tcaggaagct
     6781 gccgatgctt gtgtagccac cctcggaacc accccatagt attcctgtaa taaaatagga
     6841 tggggagcgt gacgctcccc atcctatttt caatctactt aagcttcaag ctttataata
     6901 tccacacttg gccgcgaatt gcgctgatat tcttgaagac tcgccctgtt ccccatggtg
     6961 agcaaacaaa agaggtttat tatggaaagc gataatttcc tgcgacgaaa cctgcccaac
     7021 atcctatgga tcgctgtaat cgtctcgatt tatttcgttc tatcatcgtt gatcgctccc
     7081 ttctcagacg gcatcacctt aagcagcaag atcgtaggtg agcggcttca ggaaattggc
     7141 agctttcttt tcgcaatcct gggcggactt gcaatcatcg aaatttattt ttatcaaatt
     7201 ttcttccgcc agccgcttaa ccgcaccgca acaatcataa caagagccat ccaagttgcg
     7261 atgggtctcc tcaccttcac gttcatcatt tactttttca atctaaccga cctaatcaaa
     7321 ctcggcgaca gcgccatcgg caagtttgta aacatgcccg tggactcgtt tgcgaacctt
     7381 caaaagtccc tcatcagctc aaaggttgcg acaaaagact cggcaaaaat ggtcgcaggc
     7441 atcatctttg ccctcggcat cctggtgctg tatgcgatca ttcaattcac cagcaaaccg
     7501 cgcaacagcc gcgtggggct ggcctatctg ctgatcatgc ccgcttttat cggcgtgctc
     7561 ttcctgatca tctacccctt ccttttcgag atcaaactcg cattctccaa tgcatcgctt
     7621 ggaacccaag cgactaagaa tgcccaatac agtctgcttt tcgggtggga aaatctcaag
     7681 acactcttca caggcacggt cgcgaaagac gcgaaattct tcgaggtgtt ctggcgcacg
     7741 atcctgtgga cggttatcaa cgtcaccttt cacgttttgg gcggtatggg gctggccatc
     7801 ctgctgaaca gacccttgaa gttcaaaggg ctttatcgca ccttgctcgt cattccctgg
     7861 gcgattccaa ccacgattgc agccatggca ttacgcagtg aatttgacag ccgctacggc
     7921 ttgttcaata tcctgctggg caacctcaac acttggttga ccacggtcag tcaaaacacg
     7981 gcaaatatta tagggatcgg aaatggcttc gcctggctgg cggccaccat cggccctgtc
     8041 agttggaaac aggacccatt ttgggcgttc gtttcggtgt taatcgcaaa tatctggctg
     8101 ggcattcctt ttatgtccgt catcattctc ggcgggttgc aaagcatttc gcaggaatat
     8161 tacgaagccg cagagatcga cggcgccacc cgatggcaac aattccgtaa catcaccctc
     8221 cctctactgc gccccgtgct gacacctgcc attattttag gcgtcgtctg gaccttcaat
     8281 aagttcgaca tcatctacct catcacgcag ggcggcccgc aggagaaaac ggatattctg
     8341 gtttcctcca tgtataaagc cgcattccag ttttatcggt acggcttcac tgccatgttc
     8401 gcactggtaa tctttgcgat cctgtttatc ttcactctct tttatcttag ggtcagcgat
     8461 gggcttaagt ccgcaactga ataaggagca tcacatgaca acgaatccaa tttcccgctt
     8521 ctttcgctgg ttcttttatc ggtcacgcgg cgacagtccc ttcaaaacca ttttgatcca
     8581 tgcgaccctt atcatcgcct gtgtcatcgc tgtatatccc gtcttgcgcg tggttaccat
     8641 ttcgctgcgc cccaatgata cactcctgac caccgacctc agaatcatcc cagaaaacgc
     8701 cacgctggac aattacaggg aagtattgtt cggcgacgcg gaaaagaatc gccagtccga
     8761 tttcttcctc tggatttgga actcggtctc tgttgtagcg gtgacttcag tgatcagcgt
     8821 cattctggca gcgatcagcg cgtatgcgtt ttcgcgcttc cgttttcccg ggcgctcggc
     8881 agggcttata ttcctgctga caacgcagat gatccctgcg ggtatgttgt tgcttccctt
     8941 atttatcatg atcgccaaat tgaaaatgat caatacgcct ctgggtctga tcatcgccta
     9001 tgcagtgacc tccgtgccgc tcaccatttg gaccctcaaa ggctactacg atacaattcc
     9061 cgttgacctg gaagaagccg cgctgatcga cggcgcaagc cgcctgaccg tattcaccaa
     9121 gattatcctg cctctctcca cacccgcact ggcgattgcc ttcctattct ccttcatggg
     9181 cggctggagc gaatatctgg tggcacgcgt ggttttgcaa aagaatgaaa tgttcacctg
     9241 gccgttgggc atcttcacct tcgcacagca attcacggtt tcgtggggtc aattcgcagc
     9301 cgcatcggtt ttgatcgcca ttcccatcat ggcgctcttc ctctattctt ccaagtggct
     9361 catctcaggt ctcacccttg gcagcgtaaa gggataatac tttcatcatt gaatattccc
     9421 tttctttcct tgagaggaag ggaatatttt tgtctattga ctgtcatgac caaacggatt
     9481 tttctctccc tgctgctttt cctgctcatt tcttcctgcg ctcccgccac gccgacaact
     9541 gctccagcgc caacgacagt tgctcctcct aaaatttggt ggagcgatgc cgtgttctac
     9601 gaaatttttg tgcgctcttt caacgacagc aacggcgacg gcgcgggtga tttcaacggc
     9661 gtcacccaaa agctggacta tctacaggaa cttggtatca ccgcactctg gctgatgccc
     9721 attttcccct ccccctccta tcacggttac gacgtcacag actattacgg tgtaaacccg
     9781 caatatggaa cgatggatga cttcaaaaac ctggtggcag aagcccacaa acgcgacatc
     9841 cgcatcatca ttgatctggt catcaatcac acatccgaca aacacccctg gttcagggaa
     9901 gcaaaaaaag acccccaatc gccctatcgc gattggtata tctggaacga gacagacctg
     9961 aaatatgaag gcccgtgggg gcagcgcgtc tggcactcct ctacaacggg tttttattac
    10021 ggcatctttg aagccttcat gcctgacctg aattacgcaa accccgctgt taccgaagaa
    10081 atgaaaaaaa tcgccgcctt ctggctggcc gatgtgggcg tggatggctt ccgcctggat
    10141 gccgccaaac acctgatcga agacggcatg aagcaggaaa ataccaatgc cactcatctt
    10201 tggtatcaaa atgaattcta tcccgcatac aaagcagttg accctgaagc catgaccgtc
    10261 ggtgagttgt tcggcgacgg attaaatacc atcgccaatt acatcaagaa cgatcaattc
    10321 gacctggctt tcaactttca acttgcggcc agtttcctcg aagcggtcaa cactgaaaaa
    10381 tcaggcgacc tcgccagaac tctgcaagta agcgacaagg ccatccccga tcatcaatac
    10441 gccacgtttt taacgaacca tgaccagaac cgcgtcatga cgcagttggg cggagacgtg
    10501 aacaaggcca aactggccgc ctttttaatg cttacctcgc ccggcacacc gttcatctat
    10561 tacggcgaag agattggcat gcaggggaaa aaaccagatg aaaacatccg cctgcccatg
    10621 cagtggacct cgggagaaaa tgcgggcttt agcataactc gtccgtggcg ggcgcctgcc
    10681 agcgattatg tttttataaa tgtcgatgcc caaacaggtg ccatcgactc gctgctcgaa
    10741 cactaccgcg cgctgatcca attgcgcaac gcaaaccccg ccatgcagac aggtaatctt
    10801 atttcgttga aatccagcca tccttcggtg tattcggctt tgcgaatcaa tgaaaatgga
    10861 atgtttctga tattggcaaa cctctcaaaa gaaacgatca gcaaatataa tatcaacctc
    10921 aacggggcag gcttagcaga atccgcctac agtgtggaga ccatgtttgg ggctgggcag
    10981 gccgcaggtc cagttggaag cggcgaagcc ttccaggaat acaagccgtt cgagaatctt
    11041 gatccgtatg caatgtacgt tttgaaactc atttcaaagt aacgaggata aataatgtcg
    11101 acaccagaat gggtaaaaga tgcagttttt taccagatat tccccgatcg tttcgcaaaa
    11161 agcagccgca accctgcggc tgcactgccg tttgagccgt gggactcagc cccaaccacg
    11221 catggattca aaggcggtga cttgtacggc gtgctcgaaa aattggatta cattcaagag
    11281 cttggatgca cagcgatcta tcttaacccg atctttgctt cggcttcgaa tcaccgttat
    11341 cacacctttg attactacaa cgtggatccc ttgttgggcg gcaatgaagc gttgaaaaaa
    11401 ctgctcaaag aaatgcacaa gcgcggtatg cggttgatcc tggacggtgt gttcaaccac
    11461 gcctcacgcg gattctggca attccatcat gtgctggaga cgggggattc ctcgccttac
    11521 aaagactggt tccattttga ccatgaccgc ctgacaggta aaaagcattg gggcgcctac
    11581 cccacccccc acgaagataa aatccttcag cacgaagaca gcctgaccgc catcggttac
    11641 cgcgcctggt ggaatatccc tgcgctgccg aaattcaaca ccaagacccc cgccgtgcgc
    11701 gaatttttat ttgaagttgc cgagtattgg atcaagttcg gcattgacgg ctggcggctg
    11761 gatgtgcccg gtgagatcga tgatgacgag ttctggcgtg aattccgcag acgggtgcgg
    11821 gcgatcaacc ccgatgcata catcgttggc gagatctggc atgaggccca gcgctggctg
    11881 cagggtgacc agttcgatgc ggtgatgaat tatctcttca catcggcttc actcagcttt
    11941 tttgcggggc aacacctgga tatgggcgtg atctcgcagg cgggcggaat caaggaccgt
    12001 gtccgtgcgg tgaacgcgta tgaatttgcg aatgaaattg accgcatcct gaacctgtat
    12061 ccgcctgata tcacttcggc gcaattgaac ctgctcgata gccacgacat gccgcgcttc
    12121 ctttcctgcg caagtggaga taaagcggcg ctcaagatgg cctggctgtt aatgtttgcc
    12181 ctgcctggcg caccttgcat ttactatggc gatgaggtcg gtgtagacgg acggcacgac
    12241 cccgaatgca gaaaggcatt tccgtgggac gaaagcaaat gggacaaaga gctgcttgag
    12301 ttcgccaaag cctgcacaac cctgcggacc gaacatgcag ccctgcggcg cggcgaatac
    12361 aagcgcctgt atgccgaagg ggaagttgcg gcgttcatgc gttcatacaa gaacgaacat
    12421 ttgactgtgg tgtttaacac tgcaacagag gaccgctcca ttgaattgca ttttgagaag
    12481 aaaccgcggg tactgctcgg aaatgcctct ttggacggga atcgggtgac gatccctgcg
    12541 cggagtggaa ttgttttgaa gtaggcaaaa agaaaaagac caaccactga ggcacggaaa
    12601 cacaaagaaa gccattaaaa ttcggtgcct cagtggttca agtttgttga ttaccgaaaa
    12661 ctgtcaggca ggtagtaaat tttacaggct ctttggtgtc tggtggggtc tcctgctttt
    12721 tttaccaccg agacacggag actcagagaa aacctttata aatctccgtg gtgaacactt
    12781 aatcctgaag aaccatttta taaaaatatg ctctcggcgg cgtccccgcc gccgaggacg
    12841 gcggtttgag cgagaaacca aaaaataaaa aacatggaaa ccttttatga ctgaaaatga
    12901 agccccaacc cctgctgaaa ttctcaaatc cattcttgaa ggcttgagcg gcgccgatgc
    12961 ttcggtgcgg ttggaggcca ttgagaaact gcattcgatc aattacagca gtgaggccat
    13021 ttacatcgag ttggaaaggt tggcgatcca tgatgatctg gaggaggttc gaaagcgcgc
    13081 gttggaggcg cttgaccttg ccacccataa acatgtgcgc ggaagggtca accgattgaa
    13141 tgcgggtgac cgcactgtgc ttgtggagga gatcggtgag tgggagagcc tcgggctgtt
    13201 agacctgacg cgcgctgcgg tgttgagaag gcgttatgac ttcgacaaaa gggcgaaggt
    13261 cactgccgat gcggttatga gcgagaaacc tgccacggtt gaaaaggcag aacccgcacc
    13321 caaggcagag cctgcccccg ctgagccgcg ccccaccctg atgcaaaccc tgttaagcga
    13381 aacgagcatc aagatcgctt tatatttggg ggcattcttt gtggtggcat cggcggccat
    13441 tttggcggcg gtttcaccgg agctgcgcct gttcgtgttg atcgtggctt cgcttggttt
    13501 cggcgggctt tcgctgggta tccgcaagcg gctgccccag ccgagttttg cgcttttcat
    13561 tgtgttctcg ttcctgctgc cgatcacggc gaatgtgctg caagatacgc tcggcctgac
    13621 ggatgtggtc aaggcggtgt attgggtggt gatctcattg gggatggcag tgctttgggg
    13681 cggaagcatc tggctgtatg agtcgcgggt gttcagtgtg atgagcttcc tggctttcgt
    13741 gctggccttc tatcgaatcg gggaggtgtt cgaggcggaa ggagaagtgt acggcctgct
    13801 gatgggcgcg gcgactctga ccgggcttgg gttggtttgg gtgctgaaga agtggcggga
    13861 tgataagttt gcgctgccgc tgttcctgag cacactggcg gtgcaggcat tgatcctggg
    13921 cgggacactg gtcttatttg caattcaatt gttcgatggc aacgattccg cgctttggaa
    13981 tctggcctcg atgctgacat ggggactggc tttcatattc cttgtgcttg ccgatgtgct
    14041 tttcccgttc gtgttttttg cctggtttgc ggcactggct ttggtgccga tcccgtggtt
    14101 tttgggggca gccttcgaca tggaattcaa cgggctggcg atcttgtttt ttgtttgggg
    14161 gctgatcctt tctgtcttta gcgaggcagg tttcaggttg gaatggacgc ggaagttcag
    14221 tctgcctgtg gcgatggtgg cgatccctgc ttttgtgctg gcgttgatca atgcgttcag
    14281 tgagggagtt tcgctggggt ttggaatcgc cctgggtacg acattggtgt atgcggtttt
    14341 gcagttgctg cgcgggcgtg agtgggtttg ggcgctggcg ttgctgaatt ttttcattgc
    14401 ctattttgcg ttctttaatc tgacgttgat cgaaccgttg aaagtgtatt ttggatatca
    14461 ggcacttggg ttgagcctgc ttttgatgct gcccgggctg tttggaaaaa acgatctaag
    14521 aaccgcttcg acatggaaat tgccgaccct ggcgtttggg gtgatcttta cattgacggc
    14581 gacggtggcc tttgtcatcg gtgagaattc gctccatgcc atggtggggt ttggagtgtt
    14641 ggcggtgttc ttcacggcgt atgcgctggt gcagggttcg gcgttttatg gatacctgcc
    14701 tgcgagcttt ttggcgcttt cggcgttgtt cggggtggag tatctggata aggattggtg
    14761 gctgcccgtg ttgacgacgc tggcggtttt gtattatgcc tgtggggcgg cgctgctttc
    14821 acgcgctggc tggtcgcgga tgctgcggaa cagcggtttg gtgctgggca ctttgatcgt
    14881 ggttgcggcg gcggctcaaa tgaaaggggc gggcggatgg tacgccatgg tggtgggttt
    14941 gatctttgcc gtggaaatgt atttgagcgg cgagggtctg cttgagattg cgccgccgat
    15001 ctttttcagc gctggggtga tcctggtgca gcgtgccatc ccgacgccga tgggcggcac
    15061 acatcagtat cttgtccata gcctggtgtg gctgggctgg gatctgttga tgcatttgac
    15121 gttcaagcag gcgcgtccat taagttggct ggtgcgcggc ggcggtgcgt tggtgaccct
    15181 gatgaattac ctgatcctga tggatgtggg aagcgcgaag gctggggcgc tggattttgg
    15241 aatatatacg cttcttttct taacggtgaa cctggtctat cgccgaccca tcttgttcca
    15301 cacttttggt gtgactctgc cactgttcgt gatgttcgtt tttcgtttgg tgggcgtgga
    15361 gaagtggatc caccctgtga tcttcgtggc aatgggattt tatgcggttg gttttgcgct
    15421 gcggtggatg aagctggagt gggcaaagga ctgggataag gctttgattt tggaaggatt
    15481 gggttggggt atggtgattt cgatcgcggc gccaacgctg ggcgggcttg atgcttcgat
    15541 ccctgtggcg ttcgcggcaa cgttgtgggc ggcggaggca ttcgctttac gagatgtgcg
    15601 gttgggcttc cctgcaaacg ggttgtattt gctggcgtat ttcatcattt tgaatgaatt
    15661 gaacgtggac cagccgcaat tctattcgat gggtgcggca gcgttgggaa tgttccagca
    15721 ttatttgctg acgcggtcgg ggagcaagac agggactttc gtgatgggaa tgctttcaca
    15781 actgaccttg ctggggacaa cctatattca gatggtggat tcggagaagc tgatctattt
    15841 cgtggtgctg ttcttccagt ccattgcggt gttggtgtat gggttggtga tccgctcgcg
    15901 gagtttgacc ttcacgccga tcgtgttggt ggttttaggc gtggtgacgg tgttgtacac
    15961 tgccttgaag ggtttgaatg cagtggtgct gatcggttgt acgggcgtga tcttgcttgg
    16021 tctgggaatt gcggcggtgt tgctgcgtga gcgaatcgcg cggttgggtg agcggttgag
    16081 cgattggagg gcgtaggggg
//
LOCUS       DAOQJC010000092        15897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1750_length_15897_cov_9.068931, whole genome shotgun sequence.
ACCESSION   DAOQJC010000092 DAOQJC010000000
VERSION     DAOQJC010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15897
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1750_length_15897_cov_9.068931"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PLE39_13485"
     CDS             <1..792
                     /locus_tag="PLE39_13485"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNE69391.1"
                     /translation="DFTFADTTDPENLAEALTPSTKIVWLETPTNPLLAVSDIRAIAE
                     VVKHHPNKPLLVVDNTFATPYLQRPLEMGADLVVHSMTKYLGGHSDVVGGAIIGKDKE
                     LGEKLAYLQNAIGGIQGPMDSFLVLRGIKTLPIRMDRHAHNAERIVAFLEKQKNVKQL
                     IYPFHESHPQVQVAKRQMLNGGGMISFVMKDGRDAAVKVAESTKIFTLAESLGGVESL
                     IEVPAAMTHLSTQGSKLEVDPGLVRLSVGIENVEDLLGDLEQALR"
     gene            complement(875..1276)
                     /locus_tag="PLE39_13490"
     CDS             complement(875..1276)
                     /locus_tag="PLE39_13490"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE69392.1"
                     /translation="MLYGILNLTTNEFSYARAGHEPPLILQPDGTVERLPYRPGMALG
                     LWDSILLDEHAITLPPGSTLLLFTDGMTDCRDPNGVAFGLDNIKSVLETLPAASAQQV
                     CDHMLDTLVKYQNGSKQDDDVTLVAIRATGK"
     gene            complement(1252..2106)
                     /locus_tag="PLE39_13495"
     CDS             complement(1252..2106)
                     /locus_tag="PLE39_13495"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE69393.1"
                     /translation="MSNTLLSKIPLFAGLPRKELDQILAAMEKKEMADREILFKEGDP
                     GEHFYIVMKGNLEVLMAAGQAEELLLNVMSEGEHFGEMGLIMPGGHRTATVRARGEST
                     LLSMSRETFLGLTQKHPELSGSMVRVLSQRLDATNTQTFHELTEKNRKLQTAYDELKA
                     AQAQIIEKERLERELQVAAEIQLSILPDKLPEPPNFDFGARILPARQVGGDFYDVFYV
                     GEGLIGVLIGDVADKGVPSALFMSRAHALVMAEADTGLSAGSWLTAISRVCRNPLNLS
                     QCYTAFLI"
     gene            complement(2108..2752)
                     /locus_tag="PLE39_13500"
     CDS             complement(2108..2752)
                     /locus_tag="PLE39_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNE69394.1"
                     /translation="MAKHLVLVAGNIGAGKTTLTERIGARLGWWTGYESVTDNPYLSD
                     FYGDMHAWSFHLQIFFLGHRADQYLEASRDARSAILDRSIYEDAHIFARALHHMGNLG
                     ERDYLAYRRLFEVVVNGLPRPDLLIYLKAPVPTLMDRIRRRARNMETGITPDYLSLLD
                     SFYSEWLSAFDLCPVLTIQTDDLDFAHHPGHLDLVVQRIQDKLAGKEVMDLTQK"
     gene            2863..3270
                     /locus_tag="PLE39_13505"
     CDS             2863..3270
                     /locus_tag="PLE39_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69395.1"
                     /translation="MKIRDASGWTIFIFGALAFILGLMGLIRPEILLSILGFEVIDRA
                     ARASSDYTIVFMTASSMASLNIGIYYVLAAFNDVKIFYRWTVPFRTLTFIVFTTTVIT
                     GIAPMRFFGVGLWEFLGALSTGIALYREDRAKK"
     gene            3286..3648
                     /locus_tag="PLE39_13510"
     CDS             3286..3648
                     /locus_tag="PLE39_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69396.1"
                     /translation="MKSKWIEHNGKKILYQDFSNLFFNTQAVAEELSQVQQVVIGEPE
                     NSVLVLSDFRNTEVTNALMPLLNEASRNTKSHVRKTATLGITGVKRTLGDLLSRITGQ
                     PIMYFENEKDAKEWLVKD"
     gene            complement(3979..4779)
                     /locus_tag="PLE39_13515"
     CDS             complement(3979..4779)
                     /locus_tag="PLE39_13515"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE69397.1"
                     /translation="MNTIRKNLYLFWQGTIISYRALFAWLRPVTYVASKVLMPLAQMF
                     FFTFLGSYATNGKNIDFYVIGNAIQIASVSGIFGVTMSVGGDRDAGTLPYLFGTPANR
                     FMIFFGRAFMHVIDGALGVVIALTWGVLLMGLDLSRTDLPALGLTILITTFSTCGLGL
                     LMGCLSLLTANVMFVNNFVYFSLLVFSGANVPIATLPEWMQAVSTILPLTRGIASARA
                     LAAGASLSEVSPLLLSEAGIGLFYGVIGYFLFSWFEIQAKQKGTLETV"
     gene            complement(4782..5579)
                     /locus_tag="PLE39_13520"
     CDS             complement(4782..5579)
                     /locus_tag="PLE39_13520"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE69398.1"
                     /translation="MNYANLFRYWRTVLTVAEMSIRTQFSDSFILFAIVFQPMIIAIL
                     ALFMLREKGGDYAMFVVVGSGLTGLWSSLLFVSGNSINSERWTGTLESLVGVPTPFDV
                     IVFGKNLANVFQSLLSMVTSYTLAAFLFGYSLSFTQPLLFMVTLLFSMFAFICFGLTI
                     APVFVMYRSVQQWQNAMEFPVYILGGFLFPIAMLPGWATPISYLLPPYWAAQALHGTS
                     TAMMPFEQILFCWGMMIVFSVVDLVIASQLFKVMLYKARVDATLDVD"
     gene            complement(5576..6547)
                     /locus_tag="PLE39_13525"
     CDS             complement(5576..6547)
                     /locus_tag="PLE39_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE69399.1"
                     /translation="MNAVEVNQLRRIYRAQIGVFNRSIKEVVAVDEISFDVKQGELFG
                     LLGPNGAGKTTTVKMLTTLLIPSLGSASVAGFDVVKQANEVRKRIGFIFGGERGLYWR
                     LSGDDNLRYFASLYGVDPDISKKRIPYLMELVGLKGRGNERVGGYSRGMKQRLHVART
                     LLHDPDILFLDEPTIGLDPVGAREFRAVILDLQAQKKTILLTTHYMFEADALSDRIAI
                     INKGRVIALDTPGGLKQHVSDLSVVEVETFGAPDSTLEKLRSLPFIDSLSVEDREQKQ
                     MLLVQTKRGAEAVPDVMAALDGQRIGRVIVREPTLEDAYVRLVGGQS"
     gene            6672..7298
                     /locus_tag="PLE39_13530"
     CDS             6672..7298
                     /locus_tag="PLE39_13530"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNE69400.1"
                     /translation="MKTRLILFSTLILGALLFSCESNKSTPTQPVELDTDTLRTEAVS
                     TFASALTETLAAVPTASPAPTEKFTATPVVLTATLETPAAADPCYDLIWIEDVTIPDG
                     TNLKPNETFTKTWLVQNSGGCAWPPGFTFQNVGGDPMRGETFILSEAIPVGAKREISI
                     QLAVPTDQNGLIQSSWRMADANGIFFGDTLTVNITVGNILTPTSTTSP"
     gene            complement(7308..9539)
                     /locus_tag="PLE39_13535"
     CDS             complement(7308..9539)
                     /locus_tag="PLE39_13535"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNE69401.1"
                     /translation="MKIFSLRSFLTWLILLLAMGALTAGLKDGILDVQDAAFFSVAAF
                     TVTLAFLIGFGASSPRRAWSIVLVSGVLFAVIESARLMEPFKLIIRAIPPFERDLLLW
                     FIERDSPEAIFPDASIFLIQFNELVRRADTFFTLIFNAALARPMVREFIWDLPILFCA
                     AWAGWKTSRSNQALLALIPALVLQTFILEYTGKDLFPLQLGIFTLLMLIGVNHNWSIA
                     SGNGKTARETYSAILILSIALTMGSGFVPSISISEAAKKLIRKDDIGKALGLDKESVQ
                     AYVISGLSGLPRQHLIGMSPTLAETVMFTVQTGELPPTENSIIAQAIPRHYWRWLTYD
                     VYNGQGWSTSEVSSVTYSAGETILREGASFQTLHQQVKKSFSEDNRLYWAGQLGGVDQ
                     PVSVNWRDPLSEADMLGGTTQAQIYSADSLLVIVSADELRASPAVPIAGPQEKYLSLP
                     VTVPQRVHALAESLTADFNNPYDKAKAIERYVRTYPYSLDVTSPAPNQDVADYFLFDL
                     KKGYCDYYATAMVVLARSVGLPARLVVGYANGIYNARSAQYTIREADAHSWVEIYFTG
                     VGWVEFEPTAGQLELNLPDDLSEAETLPPPVFPEPVSRLRDFDKQGFFPERNLLPPIL
                     AAVLSVLFVGIWFLYVQGLLRRHETIGSIYEYVYQHGRKIYDASPANETPTVFAQKLA
                     ARLKSGNEWIAPAPDEVRFLTALYLQETYSAHPVSQDERREAIKVWRKLFWRLLYAQF
                     KRI"
     gene            complement(9549..10805)
                     /locus_tag="PLE39_13540"
     CDS             complement(9549..10805)
                     /locus_tag="PLE39_13540"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNE69402.1"
                     /translation="MMRIRLRLPVFAALITVFLIAQLVQPSQTWMFLLTVFSGIFISA
                     YLWTRSLGMSLQLRRETRLGWIQVGGQIEERITLSNTSVLPAAWIQFKDHSTLPEFHA
                     SRSTSISAGFFDQWIVTADSNQRGLFYLGDADIVTGDPFGIFEATIHASERTSILVLP
                     QVLTLPEFSILPSGSFGDGQPRRNAPEQTIHASTVREYVHGDSVRMIHWPTTARTNKV
                     FVRLMESAPAGDWWILLDLDKKNLFGTSWDSIEEQSVMLAASLASRGLRDRKAVGLVT
                     NSKDPAWIHPQKGEGQRWEILQSLALAQPAELTLTQTLERIQPLLGRRHSLIIVTASP
                     KPDWLKSLPPLIKSGIVPTVFLFDAATFGGNESPASVAANLDGLGIKYHLISHGLLEP
                     PKVLPRQQGGWKWRSTPTGEIVPIRS"
     gene            11112..11561
                     /gene="nrdR"
                     /locus_tag="PLE39_13545"
     CDS             11112..11561
                     /gene="nrdR"
                     /locus_tag="PLE39_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNE69403.1"
                     /translation="MRCPYCKHHDSKVLDTSHDSHGGIRRRRECLKCRQRFSSYERPI
                     LATPLIIKQDGGREEFDREKLARGIRISCAKRPVSAADIERMIGQVETQLQKMGKAEV
                     SSRVVGDLVMTTLKEVDQIAYIRYAIVYLGLDDLQSIRTEIDQLLED"
     gene            11723..14314
                     /locus_tag="PLE39_13550"
     CDS             11723..14314
                     /locus_tag="PLE39_13550"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNE69404.1"
                     /translation="MVAKSAEPKVKNGLLPTPAMAKGLPKASLTDNARQVLMKRYVRR
                     GDDGKPAENVEEMFWRVAHHVAKVEEQWGADVAQRTVEYYHLLSSKKFFPNSPTFTGA
                     GTPLGQLAACFVLPITDDMGRDGAGIFQTLRDAALIQQTGGGNGFSFSRLRPKGALVQ
                     TSAGQATGPVGFLRVYDHAFGEIAQGGTRRGANMAVLRVDHPDVEDFVTCKINENSIT
                     NFNISVGITDAFMQAVKDDAEWPLRFPDLGEMKQKGFSGTLEQAEAAGITIHTYRKVN
                     ARELYNKIVKQAHHNGEPGVLFLDAANRGNPVPHLYPLEATNPCGEQWLGPYENCCLG
                     SVNLNEHCGPDGSVDWETLRQSVVTATRFLDDVVEANAYVPAVPQLKEAAHRARRIGL
                     GIMGLADLMYHAGVRYGSKEGQEFGAQVMEFVRFHAMKTSVELAEERGPFPAIEGSIY
                     DMDDMKWHAPKSLVDYQHNWGRPEVKWDAIVSGIKQHGIRNAAQTTVAPTGTIATVAG
                     CEGYGCEPVFALAYIRHVNDNGKDLKLTYASPRFDEALKKLGLGEEKRQEIVEQVMNE
                     GTCQNISEIPQAVRDTFVVSGDITAEEHVRMQGALQAFVDNSLSKTVNFHENATEEDV
                     AKAYMTAWELGCKGITVYVTGSRDKVVLETKATADKKAPASAEPKTVSSPQGAPVQQA
                     APTIWHGTKKPRPKALTGKTYNIETPVGKAFITINENGGTQPFEAFVNTAKAGSEIAA
                     VSEAIGRLISYILRMASPIAPVDRMREIVVQLIGIGGGRSLGFGINRVRSLPDGIGQV
                     LDQYLREKDGAPVEEVKTNGNGNGHHEEPHAHTMKIGDICPECGEAAVVNEEGCRKCY
                     ACGFSEC"
     gene            14394..14768
                     /locus_tag="PLE39_13555"
     CDS             14394..14768
                     /locus_tag="PLE39_13555"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNE69405.1"
                     /translation="MKAATFSVKTKIRPVGFPPVAKTLPGAIERIVSELKPEKIILFG
                     SYAYGKPTPDSDVDLLVIMNTKAKEIDRYVKVSNLLYPRQFPVDIIVKTPKEIAAERK
                     KQGNFFMREILAKGKVLYERGK"
     gene            14752..15141
                     /locus_tag="PLE39_13560"
     CDS             14752..15141
                     /locus_tag="PLE39_13560"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNE69406.1"
                     /translation="MSAANNVLEWVSYAEEDLIMAKTALKRSKPLTTASCFHSQQCAE
                     KYFKAILVARNIEFPKTHDLLILNTLCANTGIITGFKITDLGRLSGYAVHTRYPGNQP
                     TPAAAREALEIANTIRQFARIFLGLKK"
BASE COUNT         4158 a         4131 c         4031 g         3577 t
ORIGIN      
        1 gactttacat ttgctgacac gaccgatcca gagaatctcg ccgaagcatt gactccgtct
       61 acgaagatcg tttggctgga gactcccaca aatccgctgt tggctgtcag tgatattcgc
      121 gcaatcgcag aggttgtaaa gcatcatcct aataagcctt tattggtagt ggataatact
      181 ttcgcaactc cgtatttaca gcgtccgctt gaaatgggcg cggacttggt cgttcactcg
      241 atgacaaaat atctcggcgg gcattcggat gtggtcggtg gcgcaatcat cggcaaggac
      301 aaggaactcg gcgagaagct ggcatatttg cagaatgcca tcggtggaat tcaagggccc
      361 atggattcgt tcctcgtgct gcgtggaatc aagactttgc cgatccgcat ggatcgtcac
      421 gcccataacg cggagagaat cgtggcgttt ttggaaaagc agaaaaatgt caaacagctg
      481 atctacccat ttcacgagag ccatccacag gtccaggtgg cgaagcgcca aatgctcaat
      541 ggcgggggca tgatctcgtt tgtgatgaaa gatgggcggg atgcggctgt caaggtggcg
      601 gaatccacaa agatatttac attggcggaa tcgctcggcg gggtcgaatc actgatcgaa
      661 gtgccagcgg cgatgacgca cttgtccaca cagggttcga agctggaagt tgaccccggg
      721 ctggtgagat tatcggttgg gatcgaaaat gttgaagatt tgttggggga tctggaacag
      781 gcactgcgat aaagttttac tgtcaggaat cacaacgggt cacaaaggaa aattccttgt
      841 gacccgtttt tatgtttcga ccatgcgaga tcgattattt tccagtggca cggatggcga
      901 caagggttac atcatcatcc tgtttggagc cattctgata tttcaccaga gtatcgagca
      961 tgtggtcaca aacctgttgc gctgaagccg cagggagggt ttcgagaacc gatttaatgt
     1021 tatcaagacc aaatgccacc ccgttggggt cacggcaatc ggtcatgcca tctgtaaata
     1081 acagcagtgt ggagcctggt ggcagggtga tggcgtgttc atcaagcagg atgctatccc
     1141 acaggccaag cgccatgccc gggcggtaag gcaatcgttc cacggtgcca tcgggctgca
     1201 aaatgagcgg aggttcatgt ccggcacggg cataactaaa ttcattggtc gtcaaattaa
     1261 gaatgccgta taacactgtg acaaattgag tggatttctg caaacgcgtg atatggcggt
     1321 taaccagctg cctgccgata gacccgtatc agcctcagcc atcaccaagg cgtgggcacg
     1381 agacatgaaa agtgcggaag gtacgccttt atcagccaca tcaccgatca agacaccgat
     1441 gagcccttcg ccaacataaa agacgtcgta aaaatcgccc cccacctgac gggcaggcag
     1501 gatgcgcgca ccaaaatcaa aattgggcgg ctcgggaagt ttgtctggca gaatgctgag
     1561 ttggatttct gcagcaactt gaagttcacg ttcgaggcgt tctttttcga tgatctgcgc
     1621 ctgggcggct tttaattcat cgtacgcggt ttgaagtttt cgattctttt cggtgagttc
     1681 gtggaaggtt tgggtattgg tagcatccaa acgctgactt agaacacgca ccatggagcc
     1741 tgaaagttcg gggtgtttct gggtgaggcc caggaatgtt tcgcggctca tcgaaagcag
     1801 ggtggattct ccgcgtgcgc gcacggtggc cgtgcgatgc ccgccaggca tgatcaaccc
     1861 catttcacca aaatgctccc cttcactcat cacattcaac aaaagctctt cggcctgtcc
     1921 cgctgccatc agcacttcca aatttccctt catgacaata taaaaatgct cgccgggatc
     1981 gccttccttg aaaaggatct cacggtcggc catttccttt ttttccatgg cggcaaggat
     2041 ctggtccaac tctttgcggg gaaggccagc gaacaggggg attttcgaga gcagggtgtt
     2101 tgacatgtta tttttgggtg aggtccatga cttcttttcc tgccagtttg tcttgaatcc
     2161 gctgaacgac aagatccaaa tggccggggt gatgggcaaa atcgagatcg tctgtttgaa
     2221 tggtcaatac gggacaaagg tcaaatgcgc tgagccattc gctgtaaaac gaatccaaca
     2281 gggataggta atccggggtg atgccagttt ccatgtttct agcacgcctg cgaatgcggt
     2341 ccatcaatgt agggacgggg gccttaagat agatcaacag gtcggggcgc ggcaatccat
     2401 tgacgacgac ctcgaacaag cggcgataag ccagataatc tcgttcgccc aagttaccca
     2461 tgtgatgcag ggcgcgcgca aagatatgag catcttcata aatactgcgg tctaaaatgg
     2521 cggaacgcgc atcgcgggag gcttcaaggt attgatcggc acgatgccca aggaagaaga
     2581 tctgcaagtg gaatgaccag gcatgcatgt ccccataaaa atccgagagg tacgggttat
     2641 cggtgaccga ttcgtatccc gtccaccagc caaggcgggc gccaattcgt tctgtgaggg
     2701 ttgtctttcc cgcgccaata ttgccagcca ccagcaccag atgtttcgcc atagattttt
     2761 ttattttatc atgagaaggg tttacaagta taatttgaag aggtgcggca aaaacccatt
     2821 ccaaaacatc attcaacgca ataaaccagg agacctctac atatgaaaat tcgtgacgct
     2881 tcaggctgga ccatctttat ctttggggca ttggcgttca ttcttggatt gatgggcctg
     2941 atccgacccg aaattctgct ctccatcctc ggctttgagg ttattgaccg cgctgcgcgt
     3001 gcaagcagcg attacacgat cgtcttcatg accgcctcct ccatggcatc cttgaacatc
     3061 ggcatttatt atgttctggc tgccttcaat gacgtgaaga tcttctatcg ctggacagtc
     3121 cccttccgca cgttgacatt catcgtgttc actactacag ttatcacagg catcgccccc
     3181 atgcgtttct tcggggtggg cctttgggag ttccttggag ccctttccac aggaatcgca
     3241 ctttacaggg aagaccgtgc aaaaaagtag aaacaggagt acatcatgaa gtcgaaatgg
     3301 atcgagcata acggcaaaaa aattctatac caggattttt ccaatctttt cttcaacaca
     3361 caagctgtag cagaagaact atcccaggtg cagcaggtcg tgatcggcga acctgaaaat
     3421 tccgtccttg tgctttctga tttcaggaat acggaagtta caaacgcatt gatgccgctc
     3481 ctgaacgaag cctcccggaa cacgaaatcg catgtccgaa aaactgccac gctgggcatc
     3541 acaggcgtca aacgcacctt gggcgacctg ctttcacgca tcacaggcca gccgatcatg
     3601 tattttgaaa atgaaaaaga tgcaaaggaa tggcttgtca aagattaaaa catgagacat
     3661 cccgaattta atctttcaat tctttagccg tgaatttaca cgaaaaaagc cgacccaata
     3721 aaatgggtcg gctttttata actcatccat atcttgcaat tcatttgacc acttgcatac
     3781 ttttcaagga ttgtctttga aaagccattt tgaaccacgg agacactgaa gcagtgcgga
     3841 gcctcctgtg gacggagttt ttattttttt ctctctgtgt ctccgtggct ccgtggttaa
     3901 tctttctgac ttttgaaaat attcgcgacg ttccgaacaa ttctcggctg atttcttaca
     3961 tttggggtga atcaggcttc atacggtttc cagcgttcct ttttgcttgg cttggatttc
     4021 aaaccaagag aagagaaaat acccgatcac gccataaaac agaccgatac ctgcttcact
     4081 gagaagcaaa ggggaaacct cgctcaaact ggctccagca gcaagcgcac gtgccgaagc
     4141 gatcccacgt gtgagcggca gaatggttga gacagcctgc atccactcag gcagggtggc
     4201 aattggaaca ttggctccag aaaagacaag cagggaaaaa taaacaaaat tattcacgaa
     4261 catcacattg gcagtcagca ggctcaggca gcccatcaat agtccaagcc cgcaggtgct
     4321 aaaagtggtg atcaagatcg ttaaaccgag agcaggcaga tcggtccgcg aaagatcgag
     4381 gcccatgagt aggacgcccc aggtcaaagc gatcaccact cccagggccc cgtcgatcac
     4441 atgcataaaa gcacgcccga agaagatcat aaagcgattg gcgggagttc caaaaaggta
     4501 gggcaatgta cccgcatccc ggtcaccgcc tacgctcatg gtcactccga aaataccgct
     4561 caccgatgca atttgaatgg cattcccgat cacataaaaa tcaatgttct tcccgttggt
     4621 cgcatatgac ccaaggaagg taaagaaaaa catttgcgcc agcggcatca gcacctttga
     4681 agccacatag gtaactggac gcagccaggc aaacagggca cgataggaaa tgatcgtccc
     4741 ctgccagaaa agataaaggt ttttacgaat ggtattcatg tctaatccac gtccagggtt
     4801 gcatctacgc gtgctttata caacatgacc ttgaataact ggctggcaat gaccaggtcc
     4861 acaacgctaa atacgatcat catcccccag cagaaaagga tttgctcgaa aggcatcatg
     4921 gcggttgaag tgccgtgcaa ggcctgcgcc gcccagtaag gcggaagtaa atagctgatg
     4981 ggggttgccc agcccggcaa catggcaatg ggaaataaaa atccgcccag aatgtacacg
     5041 gggaattcca tggcattctg ccattgctgc acactgcgat acatgacaaa gaccggtgca
     5101 atggtgagcc caaagcagat gaaggcaaac atgctaaaca acaatgtgac catgaacagc
     5161 aacggctgcg tgaatgaaag cgaataccca aacaggaaag ccgccagcgt ataagaagtg
     5221 accattgaaa gcagggactg aaaaacattg gccaggttct tgccaaacac gatcacatcg
     5281 aaaggcgtgg gcacgcccac aagcgattca agcgttccag tccagcgctc cgagttgatg
     5341 ctgttgcccg agacaaacag caggctgctc cacagcccgg tcaggccgct gcccaccacc
     5401 acgaacatgg cataatcgcc gcctttttca cgcagcatga acaaagccag aatggcaatg
     5461 atcatgggtt gaaaaacgat ggcaaacaaa ataaaactgt cgctgaactg ggtgcggatg
     5521 gacatttcag ccacggtcaa caccgtgcgc caatagcgga aaaggttggc atagttcatg
     5581 attgacctcc aacaagacgc acataggcat cctccaaagt cggctcacgg acgatcaccc
     5641 tgccgatacg ctgtccatcg agggcggcca tcacatcggg cacggcttcg gcgccgcgtt
     5701 tcgtttgcac aagaagcatt tgcttctgtt cgcgatcttc cacagaaaga gaatcgataa
     5761 aaggcaggct tcgcaatttt tcaagcgtgg aatctggggc accgaaagtc tccacttcca
     5821 ccaccgaaag atcgctgacg tgctgtttca agccgcctgg cgtatccagc gcgatgaccc
     5881 tgcctttatt gatgatcgca atacggtctg agagcgcatc ggcttcgaac atgtaatgcg
     5941 tggtcaataa aatggtcttc ttttgagctt gcagatccaa aatcaccgca cggaactcgc
     6001 gtgcgccaac tgggtcgaga ccaatggtcg gctcatccaa aaacaggata tcagggtcgt
     6061 gcagcaaggt ccgcgccacg tgcagacgct gtttcatgcc tcgtgagtac ccgcccaccc
     6121 gttcgttgcc gcgccccttc aaaccgacca attccatcaa gtatgggatt cgttttttgg
     6181 aaatatcggg atccacaccg tacaggcttg caaaatagcg caagttatca tcgcccgaca
     6241 aacgccaata caggccgcgt tcaccgccga agataaaccc gatccgcttg cgaacttcat
     6301 tggcctgttt caccacatca aaaccagcca cagaagcgga cccaagcgaa gggatcaaca
     6361 acgtggtcaa cattttcact gtggttgtct tgcctgcacc gtttgggcca agcaaaccaa
     6421 acaactcccc ctgcttcaca tcaaaagaga tctcatccac agccaccact tccttgatgg
     6481 agcgattaaa gacaccgatt tgcgcgcgat agattcgccg cagttgattt acttccactg
     6541 cattcatatg ggcacctgta ttattcaatg gggattatta gaacaattgt acaataattc
     6601 aatctgaaat tcaatcacga ttgttgtata cttccaacac cattaaagga gtatcttttg
     6661 gaaggcaaac catgaaaaca cgtttaatcc ttttttcgac actgatcctg ggcgcattgc
     6721 tcttctcatg tgaatcgaac aaatcaacac cgacccaacc cgtggaattg gatacagaca
     6781 cactgcgcac agaagcggtc tccaccttcg cttcagccct gaccgagacc ctggccgctg
     6841 ttccaactgc atcaccagcg ccgacggaaa aattcaccgc aactccagtc gtcctcaccg
     6901 ccacgctcga aacacccgcc gcagccgacc cctgctatga cctgatctgg atcgaagatg
     6961 tgaccatccc cgatggcaca aatctgaaac ccaatgaaac cttcaccaaa acctggctgg
     7021 tccaaaacag tggtggatgc gcctggccgc ctggattcac ctttcaaaac gtaggcggcg
     7081 acccaatgcg cggcgaaacc ttcatcctca gtgaagctat tccagttgga gcaaagcgcg
     7141 agatttccat tcaactggct gtccccaccg atcaaaacgg tctaattcaa agttcgtggc
     7201 gcatggcaga tgccaatggc attttctttg gcgatacgct cacagtcaac atcacggttg
     7261 ggaacatcct cacccccaca tccacaacct ccccttaaat cgttctgtta tatccgtttg
     7321 aattgcgcgt acaacaatct ccaaaataat tttcgccaca ccttgatcgc ttcacggcgc
     7381 tcatcctgcg agacgggatg agcgctgtat gtttcctgca aatacagcgc cgtcagaaag
     7441 cggacctcat caggcgcagg agcgatccac tcattgccgc tttttagccg tgccgccagt
     7501 ttttgcgcaa ataccgtcgg ggtttcattg gcaggagagg catcataaat tttcctgccg
     7561 tgctgatata catattcata gatcgagcca atggtttcgt gccgacgcag caaaccctgc
     7621 acatacaaaa accatattcc aacaaacaac acgctcaaca ccgccgcaag aatcgggggc
     7681 agaagattgc gttcagggaa aaatccctgc ttgtcgaaat ctcttaatct gctgacaggc
     7741 tcagggaaaa ctggtggagg caacgtctca gcctctgaaa gatcgtcagg caggtttagc
     7801 tccaactggc ctgctgtcgg ctcgaattcc acccaaccaa cccctgtaaa ataaatttcc
     7861 acccaactgt gcgcatctgc ctcacggatg gtgtactgtg cgcttctcgc gttgtaaatg
     7921 ccattggcat acccaaccac caaccgcgca ggcagcccca ccgaacgggc cagcaccacc
     7981 atggccgtgg cgtagtaatc gcaatatccc tttttcagat cgaagagaaa ataatcggcc
     8041 acatcctgat tcggtgcagg cgaggtcaca tcaagtgaat atggataagt acgcacatat
     8101 ctttcgatgg ctttggcttt atcgtacggg ttattgaaat cggcggtcaa agactcggca
     8161 agggcatgca cacgctgcgg cactgttacg gggagggaca aatatttttc ctgaggacca
     8221 gctatcggga cggcaggaga tgcgcgcaat tcatccgcac tgacgatgac gagcagcgaa
     8281 tccgcactat aaatttgggc ctgcgtggtg cccccaagca tatccgcctc tgaaagcgga
     8341 tcgcgccaat tcacgctgac aggttgatcc acaccaccaa gctgccccgc ccaatacaga
     8401 cggttatctt cgctgaaaga ttttttcacc tgttgatgca aggtctgaaa cgacgcaccc
     8461 tccctcaaga tcgtttcacc tgctgaataa gttacgctgc tcacttcaga agtggaccag
     8521 ccctgaccgt tgtacacatc gtaggtcagc cagcgccaat aatggcgcgg aattgcctgc
     8581 gcgatgatcg aattttcggt gggaggaagt tcgcccgttt gcacagtgaa catgaccgtt
     8641 tcagccagcg taggactcat gccgatgaga tgctgacgcg gcaacccaga aagaccagag
     8701 atgacataag cctgcacaga ttccttatcc agaccgaggg ctttgccaat atcatctttg
     8761 cgtatgagct ttttggctgc ctcactgatc gaaatggacg gcacaaaccc agatcccatc
     8821 gtcaatgcaa tggacagaat caaaatggct gaataagttt cgcgcgcggt tttcccattg
     8881 ccactggcaa tgctccagtt gtgattgacc ccgatgagca tgagcagggt aaagatacca
     8941 agttgaagcg ggaacaggtc tttgcctgtg tattccaaaa taaaagtttg caaaaccagt
     9001 gcaggtataa gcgccagcaa ggcctggttg gagcggctgg ttttccagcc tgcccaagcc
     9061 gcgcaaaata gaatcggcag gtcccaaatg aattcgcgca ccattgggcg ggcaagcgcg
     9121 gcattgaaga tcagcgtaaa aaaggtatct gcacgccgca ccaactcgtt gaactggatc
     9181 aaaaaaatgg acgcatctgg aaagatggct tcgggtgaat ctctttcgat gaaccaaagc
     9241 agcaagtcac gttcaaaagg cggtattgca cggatgatga gtttgaacgg ctccatcaac
     9301 cgtgcgcttt cgatgaccgc aaataaaaca ccgcttacga gtacgatgct ccatgcacgg
     9361 cgcggggaag atgcaccgaa gccgatcaaa aaagcgagtg tcactgtgaa cgcggccaca
     9421 gaaaagaaag ccgcatcctg cacatcaaga atgccatcct tcaggcctgc ggtcagagcg
     9481 cccattgcaa ggagcaaaat gagccaggtc aggaaggaac gcagtgaaaa aattttcatg
     9541 tgcatggatc aacttcgtat cggcacgatc tcaccagtgg gagtggaacg ccatttccag
     9601 ccgccctgct ggcggggaag cactttgggc ggttccagca acccatgcga gatgaggtga
     9661 tatttgattc ccagcccgtc gagattggcg gcaaccgatg caggagattc gtttccgcca
     9721 aatgtagcgg catcgaacaa gaaaacggtg gggacaattc cgcttttgat caatggcggg
     9781 agcgatttca accagtcggg cttgggcgaa gccgtcacaa tgatgaggct gtgccgccgc
     9841 ccaagcaaag gttgaatacg ctcgagggtt tgggtgaggg tgagttcagc gggctgagcc
     9901 agcgccaacg attgcaaaat ttcccatcgt tggccttctc ctttttgcgg atggatccac
     9961 gcggggtctt tgctgttggt gacaaggccg acggctttgc ggtcgcgcaa tcctctgctg
    10021 gcaagggatg cggccagcat tacgctttgc tcttcaatgg aatcccaact tgtgccgaag
    10081 agattttttt tatcgaggtc gagcaaaatc caccaatccc ctgcgggggc gctttccatc
    10141 aaacggacga agactttatt tgtccgcgcg gtggtgggcc aatggatcat gcggacactg
    10201 tcgccatgaa catactcgcg cacggtggat gcatggatgg tttgttcagg tgcgttgcgc
    10261 cgcggctgac catcgccaaa ggaaccagat ggcaggatcg agaattcggg gagagtcagc
    10321 acttggggca gaaccaggat cgaggtccgc tctgaggcgt ggatggtggc ttcaaatatt
    10381 ccaaagggat cgcccgtcac gatatcggca tcacccagat aaaaaagtcc acgctggttg
    10441 ctgtcggcgg tcacgatcca ttgatcgaaa aaacctgcgc tgatgctggt ggagcgtgag
    10501 gcatgaaatt ctggaagtgt ggaatgatct ttgaattgaa tccatgcggc aggcaaaaca
    10561 gatgtattgg ataaggtgat gcgttcttcg atctgacctc cgacctgaat ccaccccaaa
    10621 cgagtctcac gccgcaattg cagactcatg ccaagcgaac gcgtccaaag atacgcgctg
    10681 atgaatatgc cgctgaacac agtcagcagg aacatccatg tttgtgatgg ctgcacaagc
    10741 tgcgcgatga gaaacaccgt gatcaacgcg gcaaagacag gcaggcgtaa acggattcgc
    10801 atcattgaac gattataact tttcaatcca tatcactcac cctacgcaag gagggtgtgg
    10861 atctgatttt ttgatatcgt tcaaaacgat gttgcccacg ataagaattt gaaaatccat
    10921 gtcgaaacag atttatgaac gttaaatttt ttggctgtgc aatattttaa ggaaactgtc
    10981 accatgttgt cctcttgcat atgggtgggg ttggtgctaa aatgccgaac actttagcca
    11041 tatatacggg gatgcagagc ataaacgccc atatttggct ctaacatttg ttctatttat
    11101 ggagagattt aatgcgttgt ccgtattgca aacaccatga ctcgaaggtg ctcgatacct
    11161 cccacgatag ccacggggga atccgccgcc gccgtgagtg tttgaaatgc cgtcagcgct
    11221 tcagcagtta cgaacgcccc attttagcca cgccgctcat catcaaacag gatggtggac
    11281 gtgaggaatt tgaccgcgag aaattggcgc ggggaatccg catttcgtgc gcaaaacgcc
    11341 ccgtttcagc agcggatatt gagcgtatga tcggccaggt ggagacacaa ctccagaaaa
    11401 tgggcaaggc tgaagtttcc tcccgcgtgg tgggcgacct tgtgatgacc accctcaagg
    11461 aagtggacca aatcgcctac atccgctacg ccattgtgta cctgggtctg gacgaccttc
    11521 agtccattcg cacagagatc gaccagttgc tcgaagatta aaacaacgac aagggctccc
    11581 cttccaaggg gggaatgtct ttgtcgtttt tatatccaac tatggagcgc aggcttaata
    11641 gtacaaattt cagtaaatgc agatcaaaac aaatgcagac taaagtctgc actacgaaaa
    11701 taatttatca ggagagtaaa aaatggttgc aaaatccgct gaacccaaag tcaagaacgg
    11761 tcttctgccc acgccagcca tggcaaaggg cctccccaag gcatctctga cagacaacgc
    11821 ccgccaggtg ttgatgaaac gttacgtacg ccgcggcgat gacggcaaac ccgctgagaa
    11881 tgtcgaagag atgttctggc gtgtggctca ccatgtggca aaggtcgaag aacaatgggg
    11941 cgctgatgta gcccagcgca ccgttgagta ttaccacctg ctttcaagca agaagttttt
    12001 cccgaactct cccactttta ccggtgcggg aactccgctt ggacagttgg ccgcctgttt
    12061 cgtgctcccc attaccgacg acatgggccg tgacggtgcg ggtatcttcc agaccctgcg
    12121 cgatgcggct ctcatccaac aaacgggcgg cggaaatggc ttctcgttct cacgtctgcg
    12181 cccgaagggt gcgctcgtgc agacctcggc tgggcaggca acaggccctg tgggcttcct
    12241 gcgtgtgtat gaccatgcct tcggtgagat cgcccaaggt ggaacccgcc gcggtgcaaa
    12301 catggcggtg ctgcgtgtgg accaccccga tgtggaagat tttgtcacct gcaagatcaa
    12361 cgaaaactcc atcaccaact tcaacatatc tgtgggcatt accgatgcct tcatgcaagc
    12421 cgtgaaagat gatgcagaat ggccattgcg cttccctgac ctcggcgaaa tgaaacaaaa
    12481 gggattcagc ggcacactcg aacaggcgga agcggctggc atcaccattc acacttacag
    12541 gaaagtgaat gcccgcgaac tgtataacaa gatcgtcaag caggcgcatc acaacggtga
    12601 gcctggcgtg ttgttccttg atgccgccaa tcgcggaaat cctgttccac atttgtaccc
    12661 gcttgaggca acaaatccct gcggcgaaca atggcttgga ccgtacgaga actgctgttt
    12721 gggttctgtg aatttgaatg aacactgcgg gcccgatggc tcggtggatt gggaaaccct
    12781 gcgccagtcg gttgtgacgg ccacacgctt ccttgatgat gtggtggaag caaatgccta
    12841 tgtgcccgct gtgccgcagt tgaaagaagc cgcgcatcgc gcacgccgca tcggtttggg
    12901 catcatgggt cttgctgacc tgatgtacca tgcgggagtc cgctatggct cgaaggaagg
    12961 ccaggaattc ggcgcgcagg tgatggagtt cgtgcgcttc catgcgatga agaccagcgt
    13021 ggaacttgcg gaagagcgcg gacctttccc cgccatcgaa ggttccattt atgacatgga
    13081 tgacatgaag tggcacgcgc ccaaatcgct ggtggactat cagcataact ggggcaggcc
    13141 cgaggttaag tgggatgcga tcgtcagcgg catcaaacaa catggcatcc gcaatgctgc
    13201 gcagaccacc gtcgccccca cgggaaccat cgccacagtg gctggctgcg aaggctatgg
    13261 ctgtgaaccc gttttcgcgc tggcttacat ccgccacgtg aacgacaacg gcaaggattt
    13321 gaaactgacc tacgccagtc cgcgcttcga tgaggcgttg aagaaactcg gcctcggtga
    13381 agagaaacga caggagatcg tggagcaggt gatgaacgaa ggcacctgcc agaacatcag
    13441 cgaaattccg caagccgtgc gcgatacctt tgtggtttcg ggcgatatca ccgccgaaga
    13501 acatgtgcgc atgcaaggcg cgctgcaagc cttcgtggat aattcacttt cgaagaccgt
    13561 caacttccac gagaatgcca ccgaagagga tgtggccaag gcctacatga cggcctggga
    13621 actcggctgt aagggaatta ccgtgtacgt gactggttca cgcgacaagg tggttctgga
    13681 aaccaaagcc actgcggata agaaagcacc cgcttctgcc gaaccgaaaa ctgtatcatc
    13741 cccgcagggg gctcctgtac aacaggcagc tcctaccatc tggcatggca ccaagaagcc
    13801 gcgccccaag gcattgactg gcaagaccta caacatcgaa acgcctgtgg gcaaggcatt
    13861 catcaccatc aacgagaacg gcggaaccca gcccttcgag gcctttgtca acacggccaa
    13921 ggctggctcc gagatcgccg cagtctctga agccatcggc agattgatct cctacatttt
    13981 gcgcatggcc tcccccatcg cacccgtgga ccgaatgcgc gagatcgttg tccaactcat
    14041 cggcatcggc ggcggacgtt cgctcggctt tggcatcaac cgcgtgcgct cgttacccga
    14101 tggcatcggt caggtgctcg accaatatct gcgtgaaaaa gacggcgccc ccgtggaaga
    14161 agtgaaaacg aacggaaacg gcaacggtca ccacgaagaa ccacatgccc acaccatgaa
    14221 gatcggcgac atttgtcccg aatgcggtga agcggcggtg gtaaatgaag aaggctgccg
    14281 caagtgctac gcctgtgggt tttcggaatg ttagtgggtt gaaggttgca ggttgaaggt
    14341 tttggggcgg acgaatgtcc gccctttgtt tttcaaatgg taaaattcaa atcatgaaag
    14401 cagcaacatt ctcggtaaag acaaagattc gccctgtagg gtttccgcct gtagcaaaga
    14461 ctctaccagg tgcgattgaa cgcattgtct ctgaactcaa gcctgagaaa atcatcctat
    14521 tcggttccta cgcctacgga aagccaactc ccgatagcga tgtggatttg ctggttatca
    14581 tgaacaccaa agcgaaagaa attgacaggt atgtcaaggt atcaaacctg ctttacccgc
    14641 gccaatttcc agtggatatt atcgtaaaaa cgccaaagga aattgccgca gaaagaaaaa
    14701 aacaggggaa ttttttcatg cgcgaaatat tggccaaggg gaaggtgctg tatgagcgcg
    14761 gcaaataatg tacttgagtg ggtcagctat gctgaagaag accttatcat ggcaaaaact
    14821 gccctgaaac gaagcaaacc attgaccact gcttcctgtt ttcacagcca acaatgtgcc
    14881 gaaaaatatt tcaaagcgat tcttgttgcg cgaaatatcg aatttcccaa aacccatgac
    14941 ttgttgatcc taaacacatt gtgtgccaac acaggtatta ttacgggttt taagatcacc
    15001 gatctcggca ggctatcagg atatgctgtt cacacccgct atcctggcaa tcaacccacc
    15061 ccagccgcag cacgagaagc tcttgaaatc gcaaatacga tccgtcaatt tgcgcgaata
    15121 tttttagggt tgaagaaata aaaccatcgc aagcgctgta tccgtagggg aaataaaaag
    15181 ttgatttaaa agaaccacct tttactacag ataaaaaact tccccccttc aataggaaaa
    15241 taaccatgcc tgccaccttc ccatctccat ttcctgctca tcaagggttg gtctacccat
    15301 tggcacggag gtggccaaat tcttatgatg tcatcgcctt gtcggtgggt gcagccatgc
    15361 ctgatttcgt ggatattgtt ttcggctttc tcctgaacgg gtatttccgt cactggtttg
    15421 cacatggact tatttttatc cccttcaatg ttttgggcgg tttactgcta acctggttaa
    15481 taaaaaatct tttttcgaaa ttattcaaac tgaaaacacc agtcgaacca gaaaagcaga
    15541 aaggcaaggc gtccaggcta aaagtgtgga ccttttcagt gacagtcggg gttctttcac
    15601 atctgttttt tgatctgttc tctcacgata cgaacttatt attgtatcca tggtgtgtgg
    15661 atgcccactg gtttccgcaa tggtggtaca caccctggtt cgttatccca cctccgctta
    15721 gcatggggcc agcgtattca gttggagtac acacactcgt atggttggtc cttaccactc
    15781 ttgggacttt ttggttctat cagttggttt ttcaaaaaat aaaatccact agcagggatg
    15841 cgatttctcc acgggtataa tcactccatg cccaacacat ctcacccact gacacgg
//
LOCUS       DAOQJC010000093        15892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1751_length_15892_cov_8.332007, whole genome shotgun sequence.
ACCESSION   DAOQJC010000093 DAOQJC010000000
VERSION     DAOQJC010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15892
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1751_length_15892_cov_8.332007"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PLE39_13565"
     CDS             <1..745
                     /locus_tag="PLE39_13565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNE69407.1"
                     /translation="LAATAAYRIGAGLVTLAVPEPLHVALAGRLLEATWVLLPHENGA
                     ISEKARDPVLKNLERATSVLIGPGLGDFASTGKFVAGILPVIKLPMVIDADGLRHIAR
                     LKDWHKKLFDVAVLTPHPGEMSALTGLSTEKIQGNREEVAVSYAKMWGHVVVLKGAFT
                     IVAAPDGQRTVIPVATPALARAGTGDVLAGLVAGLRAQGLSAYDAAVAGAYIHARAGI
                     LAADTLGTTASVLAGDVLDMVSDVLAELE"
     gene            complement(860..1900)
                     /locus_tag="PLE39_13570"
     CDS             complement(860..1900)
                     /locus_tag="PLE39_13570"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNE69408.1"
                     /translation="MARIFDVIEYPNEMNDEIVHRFPEEGFGDFRIGSQVIVRESQSA
                     VFFRDGQALDVFKPGRHTIATANIPQLIDFIGKAFNDRTPFPAEVYFVSRKEFANKKW
                     GTSSSILVPTPGTGLGWQVLTGYGTYSFQIKDPQQFVTQIVGATGSYRSSEIEGRLRD
                     MLVSKLGDLLGETAQTTNLTVKLSSLFEELSAGVRAKAKDDFEAIGLTLKTFYIGSLK
                     PSERAVQELRERAVLDSNLYAQLQAADAMREAANNANGGAGLTAGIGAGMGIGNLMGQ
                     ALQGGMQNTSGGGAAGAKIADIMTPAEAAQVMKVTEDDILEAIKDGSLKAKKVGKAYR
                     ISKDNLESFMNS"
     gene            complement(2068..2877)
                     /locus_tag="PLE39_13575"
     CDS             complement(2068..2877)
                     /locus_tag="PLE39_13575"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNE69409.1"
                     /translation="MTASIHQALFELEKNNGSAALCTVVKSEGSTPRHVGSKMLVYPD
                     GNFIGTVGGGDLEHRVLDEAWMALGDGKPRYLHYNMADPSRGDPGVCGGQVEVFVEPI
                     LPPPLLVVIGAGHVGKAVVHLAKWLGFRVAVCDDRAEFCTPEATPGADIYHPITMDKL
                     PEHIKIDSRTVLILTTRGSSVDAVGMKPLLDSTAGYIGVIGSRRRWATTVKALKEKGV
                     SEEKIARVHSPIGLELQAETPEEIAVSILAEVMMVKAQATGMPMKAKQATG"
     gene            complement(2878..3336)
                     /locus_tag="PLE39_13580"
     CDS             complement(2878..3336)
                     /locus_tag="PLE39_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69410.1"
                     /translation="MIQKPDVFSILKNDSATASSMGGLIFTIAVTGIIYVTKALTGIV
                     PANVNPLNFIWLTLAALIYSIIVIAVRVPYVTATFEHGVEVNAQILKSSVFRTVWTLH
                     LHYIHLGQTHDVKLKQLITEKTKLMLEKKELALIVDQRNPKNILIRDVYL"
     gene            complement(3336..4679)
                     /locus_tag="PLE39_13585"
     CDS             complement(3336..4679)
                     /locus_tag="PLE39_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNE69411.1"
                     /translation="MPDQIYVIGHVNPDTDSIAAAMGYAWLLRERDGANTIAARAGAL
                     NAQTSWVLKTVGLETPVLLTDASPRFESVMRRLDTIRPDSQLGMAWTLASKTGGVAPV
                     VNEDGKPFGIINGMSLFKYFSETLGPRPGDTTVREMMAAQCKDAADTSVPKYAANAHI
                     RDALNKILRDEFNDYWVVDEHGVYVGIANQREVLNPPRLKIILVDHNEPRQAIAALEE
                     AELLEILDHHRLGNPYTHQPIRFTVDTVGSTSTLVAEMTAEAGLSMPPALAGTLLAGL
                     CSDTLILTSPTTTQRDKVAADRLSRWAFVGGSPLKGETIESYGKAVLSAGAGLSNRDP
                     KDVVSTDIKPFEGGGFKFAVAQAEVTDILQLTEHLEPLQNALNDLRDKRGLHFAMLLV
                     TDVVRGTSRLLISANAPTILEDLPYPPLSDGTRDAQGVVSRKKQLLPAVLGLLER"
     gene            complement(4780..6450)
                     /locus_tag="PLE39_13590"
     CDS             complement(4780..6450)
                     /locus_tag="PLE39_13590"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNE69412.1"
                     /translation="MTRSEIPHLIPYLVSLAVSIAVFVYTWSRRKARGAFAFSFYGLG
                     QSLWLTGFICELTSNDISTKIFWDSFQWFAGFFILISLPIFLVQYTEYKLNYPRLLFG
                     LSFIVPILFSSVLFLDQNLHWVYRNPHLSTQGFFTELLYDFTPLVYGYAFYALLILAW
                     GLRILFQRITHSHSLYQTQMIMISLGLLIPILGMALTLFDIRITPQRDMAPFTFVIGN
                     LFVVWGLFQFRMFNVTPIARDTVFEAMVEPVVILNNQNDIVDINLSMLALLGKTADDV
                     IGEPAKKIFADFPIPIKQYMQTSYARAEATFDLGGVHVYYELTVWPLYNSNKEMTGRI
                     YISHDITAQKELERELRKLNAELEDRVRARTWELADAYDTTLEGWARALELRDKETEG
                     HSRRVTDMTLKIAVAMGVAGDDLEHMRRGAILHDIGKMAVPDEILHKPDKLTDNEREI
                     IKKHPEIATKLLSPIPFLKKALDIPYSHHEKWDGSGYPQGLKGEEIPIAARIFAVADV
                     WDAVTSDRPYNKAWPREQALKYFGEQTGKHFDPRVVNIFLKLVEQGEI"
     gene            complement(6582..9551)
                     /locus_tag="PLE39_13595"
     CDS             complement(6582..9551)
                     /locus_tag="PLE39_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNE69413.1"
                     /translation="MNITLKVNGIEHAIDTAPATTLLAALRGLGYHGVKFGDEGNLSG
                     ADTVLLNGKPVNAGALLAAQAEGHDIATIEALGEHPEQGWKTTDGLHPLQKAFVETGA
                     IQCGYCTPAQILAAKALLEKNSNPSEAEVREAISGVLCRCTGYLKPVQAVMKAAAEMR
                     GDTPLDWNSINWDFGTPKDDSPQNDAPASNLTGVQVRPKVVVTPETQTWQTVGKPEIK
                     LDAVKLVQGKPAFAADFEKRGLLHAKVLHSPHAHALIKNIDASKALALEGVAAVLTWK
                     DLPRVVYSTAGQSDPIPGPLDAFSLDKKVRFVGDRVAFVAAESPEIAEKALSLIEVEY
                     EVLTPILDSRQAMGSPIRIHDELEYVNFADSNPEKNLAAHIRIDIGDVEKGFAEADEI
                     FEAEYEVPKVQQAHIEPHVCVTYWDEDDRLVIRTSTQVPFHVRRMLAPVLGLPVKRIR
                     VIKPRIGGGFGGKQEVLMEDVAAHLTIATKRPVIYEYTREEEFIAARSRHPMRVRMKT
                     GVKKDGTITANSMYALSDTGAYGCHALTVTGNTGHKAMALYVGDGEYRKAPNIRFYAD
                     VVYTNTPPAGAFRGYGVPQGYWPLDRHMEKIARAMGFDPIDFRLKNAIHPGEYHPFST
                     AWNEGREPRPEIIHTVGLEQCVAQGRAAINWDDKFGNQGWRSSVSGSKRKGIGVAMVM
                     QGTAIPYLDMGGASIKMNDDGSFNLLVGATDLGTGSDTVLAQMAAEVLGVPVEDIICY
                     SSDTDFTPFDKGAYASSTTYISGTAATKAAQIAAERIKIRAATMLGVEDHNAIQLRNR
                     HAHAPNGKSVSLAEIALDTLHKNNQEQIMGVASYVSPVSPPPFAAQFAEVTVDTETGA
                     VTVDRLVMAVDSGVIVNPLTASGQIEGGMMQALGYAVCEEMRYDEKGYAIERDFDRYH
                     LFRANEMPELETIFVETFEPSHPFGVKAVAEIPMDGVAPAVGNAILDAIGANVDDNPV
                     TPEKVWKALRAQTSAPS"
     gene            complement(9624..12536)
                     /locus_tag="PLE39_13600"
     CDS             complement(9624..12536)
                     /locus_tag="PLE39_13600"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNE69414.1"
                     /translation="MSKAFTLIKEEQVPELNSIAKLYVHKRTGARLLSVINDDENKVF
                     SINFRTTPKDSTGVAHILEHSVLGGSEKYPVKEPFVELLKGSLATYINANTFPDKTCY
                     PIASPNLQDFYNLIDVYVDAVLHPLITEQTLMQEGWHFEINDPAEPLTYKGVVFNEMK
                     GAYSSPEGVLETRIIESLFPKHIYSVDSGGDPRNIPDLTYADFKAFWENYYHPSNSFI
                     FFYGNDDPDTRLKLMEGYLKSYKRKKVKSSVKPAKPFKRAKKVESSYDAGSDTDIEAK
                     NYLVVNWTLPETTDPVLNFSLRILGSILIGTPASPLKKALLDSGLGEDLAGLGLETEL
                     RHLIFSTGLKGTRARHARKIERLILDTLETLVRDGLDPDMITAAVNTAEFRLRENNTG
                     SFPRGIALMRRVLTTWIHDDEDPFKLLGFEATLNEIKARLASDPRYFERLIQTHLLEN
                     QHRTTLRLKPDPELARRLEEEESARLAKIRESLDAKQIAEMVENTRKLKLMQETPNAP
                     EAVATLPVLKLKDLDKENKPIPIEIVQLQDTQVLYHDLFTNGIVYLDLGFDLHAMPQE
                     YLPLTEIFARALLEMGTETEDYVKLSQRIGKSTGGIYGDALSTTAYGSRENVSKLFIR
                     GKATMSQAPEMLAILKDILLTTKFDNRERLKQIVLEEKSGVESGLVPGGHIFANRRVR
                     AQFGGSGWVNDQMSGIGYLFALRELAKDIDKKWPSVLKKLEAMRDALINSRSLICNVT
                     LDAANWAAFKPRLENFIFALPDKEVTLKPFDIKAHPEKEGLTIPAQVNYVGKAANLYD
                     LGYEFDGSADVVTGYLRMAYLWEKIRVLGGAYGGFCVFDENSGVFSFLSYRDPNLDTT
                     LENYDNAAGYLKNLDPNLFNDNELTKAIIAAIGDLDTYQLPDAKGYTSMMRHLTGKTD
                     AMLQKKRDEILSTNGEDFISFGEVLEKAAKSEAVAVIGSQAAIEGSKAGLKVTKVL"
     gene            complement(12585..13679)
                     /locus_tag="PLE39_13605"
     CDS             complement(12585..13679)
                     /locus_tag="PLE39_13605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNE69415.1"
                     /translation="MTRLPVNQILQGNCIEVLNALPENSVDLVFADPPYNLQLQQDLY
                     RPNLSKVDAVNNKWDKFSSFAAYDEFTSEWLGAARRVLKENGTIWVIGSYHNIYRVGA
                     IMQNLGFWILNDVIWLKSNPMPNFRGVRFTNAHETMIWAQKKKGAKYTFNHKAMKALN
                     EDLQMRSDWHIPLATGKERIKSNGTKAHPTQKPEALLYRVLLSSTKAGDLVLDPFFGS
                     GTTGAVAKKLGRDFIGIERDRKYIQVAQKRIAAVTKSPAEALTLPEKRNQARVPFGAL
                     LEAGFLSPGQKLFFKNDPSIRAVILSNGHLKYQEQVGSIHALAKALANGPVNGWDLWL
                     YKEHGKMKAIDELREKTRKLTGSKVGKLPG"
     gene            13815..14492
                     /locus_tag="PLE39_13610"
     CDS             13815..14492
                     /locus_tag="PLE39_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69416.1"
                     /translation="MNEKSSVSAPINRLSPSELTDKIAQANVFDLSALERNRMGRLSV
                     SQFFVSLAVVGNAVFNVAGFFMLNYFIPVGFMSLRSNSFLKAAACFGVSIAVIAIFLW
                     KGKKGASIGFDRYDIKDITQFPLKSLLIIDLLLWRVAEYRGSVSSDKATGIVRTKDYR
                     DPDNPTRVNPFSEEQTVNYYSYKAGRDKFPVSFEAYKALQGKLQDCRLYYLPLSKIMI
                     NMEVLQL"
     gene            14908..15159
                     /locus_tag="PLE39_13615"
     CDS             14908..15159
                     /locus_tag="PLE39_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69417.1"
                     /translation="MRFFIKSMEDLKKTGFKLEPTALLTTKKSLEIFIKSLELLVDDD
                     IPTHLAFASLSEALVWLDEVENTQAIQRIRDELLGKLRS"
     gene            complement(15238..15690)
                     /locus_tag="PLE39_13620"
     CDS             complement(15238..15690)
                     /locus_tag="PLE39_13620"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE69418.1"
                     /translation="MMERIARCQTIVLQKGQILLICHANHQKGTIYWWLPGGGLEEGE
                     TFEQAAIRETREETGLLVRVERLLFEIEDRERTYHYQKYLTFLATPIGGELGLGFEGQ
                     AGGINRLKEVRWFPLWDESKWESGFYEPHIHPLLVKAQQELISEKTKA"
     gene            complement(15692..>15892)
                     /locus_tag="PLE39_13625"
     CDS             complement(15692..>15892)
                     /locus_tag="PLE39_13625"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNE69419.1"
                     /translation="PDFVCIEKKLIIELDGSQHLEQQEYDEERTKYLNSISYKIIRFW
                     NSEVMKNMDGVIAAIIHALEDK"
BASE COUNT         3552 a         3900 c         4203 g         4237 t
ORIGIN      
        1 gttggcagca acagccgcat accgtattgg cgctgggctt gtgaccctgg ccgtcccaga
       61 gcccttgcac gttgcccttg ctggccgatt gttggaagca acctgggtgt tgctgcctca
      121 tgaaaatgga gcgatttctg aaaaagcacg tgaccctgta ctgaaaaatc tggagcgtgc
      181 cacttctgtt ttgatcggac ccggccttgg tgattttgct tcaacaggga aatttgttgc
      241 aggtattctg cctgtcatta agttgcccat ggtcatagat gcagatggat tgaggcatat
      301 tgcccgcctc aaggactggc ataaaaagtt gttcgatgtg gctgtgctta ccccgcatcc
      361 gggtgaaatg tctgcattga ctgggttatc cacggaaaaa attcaaggca accgtgaaga
      421 agttgccgtc agttatgcaa aaatgtgggg gcatgtggtt gttttgaagg gtgcatttac
      481 cattgtcgct gccccggatg ggcaacggac cgtcatccct gttgcaacgc ctgcattagc
      541 tcgtgcaggc acaggagatg tgctggctgg gttggttgct ggccttcgcg cgcagggact
      601 ttccgcctat gatgctgcag ttgccggagc atacatccac gcccgtgcag gaatacttgc
      661 cgctgacaca ttgggcacca ccgcctctgt actggctggc gatgttttgg atatggtttc
      721 agatgtcctg gctgagttgg aatgacaaag tggtaaagac ccatgaactt atcttgccaa
      781 tggcaaaaag ccaggcatta aaacaaactc accccgaaaa attatttttt cggggtgagt
      841 ttgtttttta gtatattttt tagctattca tgaaactttc aaggttgtct ttgctgatgc
      901 gataagcctt gccaaccttc ttggccttca ggctgccatc cttgatggct tcgaggatgt
      961 catcctcggt tactttcatc acctgggcag cttcagcggg ggtcataatg tcggcgatct
     1021 ttgcaccagc ggcgccgcca ccagaggtgt tctgcatgcc gccctgcaaa gcctgaccca
     1081 tcaaattgcc gatccccatt ccagcaccga tgccagcggt cagaccagca ccgccattgg
     1141 cattgttggc agcttcgcgc atcgcatcgg ccgcctgcaa ttgagcataa agattggagt
     1201 cgagtacggc gcgttcgcgc agttcctgta ccgctctttc gcttggcttg aggctgccaa
     1261 tgtagaaggt cttcaaagtc aaaccaatgg cttcaaaatc atccttcgcc ttggcgcgaa
     1321 cacccgcgct taactcttca aacaaactgc ttaatttcac cgtaaggttg gtggtttgcg
     1381 cagtctcgcc aagcaagtca cccagttttg agacaagcat gtcgcgcaaa cgaccctcga
     1441 tctctgaaga acgatacgat cctgttgcgc caacgatctg ggtaacaaac tgttgcgggt
     1501 ctttgatctg gaaggaataa gttccgtacc cggtcaatac ctgccagccg agaccagtgc
     1561 ccggggtcgg taccaaaatg gaagatgagg ttccccattt tttgttcgca aattccttgc
     1621 gcgaaacaaa atacacttca gcagggaagg gggtgcggtc gttaaacgcc tttccgataa
     1681 aatcgatcag ctgtggaatg tttgccgtgg caattgtatg gcgcccgggc ttgaacacat
     1741 ccaaagcctg accatcgcgg aagaacaccg cgctctggct ttcacgcacg atcacctgtg
     1801 aaccgatgcg aaaatcaccg aacccttctt cggggaagcg gtggacgatc tcgtcgttca
     1861 tttcatttgg gtattcaatg acatcaaaaa ttctagccat gtttctctcc ttttagtgaa
     1921 taacgttcag attatagtcc aacagacttt cggctacaag aaccgttttt cctatttgtt
     1981 ttacgaattc caacctgaaa cgtttcaggg cttaagcaca tttttcaacg cccatgcata
     2041 cccaactcaa aagcagacgc ctctctctta acctgtggcc tgcttcgcct tcattggcat
     2101 tcccgtcgcc tgggctttta ccatcatcac ctcagccaga atgctgacgg cgatttcctc
     2161 aggggtttcc gcctgcaact ccaaaccgat cggcgagtgg actcgtgcga tcttttcctc
     2221 tgaaacccct ttttccttca atgctttcac ggtggttgcc caacgtcgcc tggacccgat
     2281 cacaccaata tacccagcgg tcgaatccag caatggtttc atcccgaccg catccacact
     2341 ggaaccacgg gttgtcagaa ttaagacagt gcgagagtcg attttgatgt gttcagggag
     2401 tttgtccatg gtaatggggt ggtagatatc agccccaggt gtggcttcag gggtgcaaaa
     2461 ctcggcacga tcatcgcaca ctgccacacg gaaacccagc cacttggcca aatgcacaac
     2521 agctttgcct acatgcccgg cgccgataac cacaagcaaa ggtgggggaa ggatcggttc
     2581 cacaaaaacc tccacttgac cgccgcatac cccggggtca ccgcgtgaag ggtcggccat
     2641 gttgtaatgt aaatagcgtg gctttccatc cccgagtgcc atccacgcct catcaaggac
     2701 acgatgttca agatctccgc cacccacggt gccgataaaa tttccgtcag ggtacaccag
     2761 cattttgctt ccaacatgtc ttggtgtaga gccttcgctc tttacgaccg tgcagagcgc
     2821 ggcagagccg ttgttctttt cgagttcaaa taaagcctgg tgaatggatg cagtcattta
     2881 taaatacacg tcccgtatca ggatattttt tggattcctt tggtcaacga tcagagccag
     2941 ttcttttttc tcgagcatga gtttggtctt ttcagttata agctgcttca atttcacatc
     3001 gtgggtttga ccaaggtgta tgtaatgcaa gtggagtgtc cagacggttc tgaaaacaga
     3061 gcttttgagt atttgggcat tcacttcaac gccatgttca aaggttgctg taacataggg
     3121 tacgcgtact gctatgacaa tgatggaata gatgagggcc gccagggtca accagataaa
     3181 attcagtgga ttgacgttgg ctggcacgat gcctgtcaat gccttcgtca cataaatgat
     3241 tcctgtgact gcaatcgtga aaatgagacc gcccatgctg gaggctgttg ccgaatcatt
     3301 ttttagaatg ctgaagacat ctggcttttg gatcattatc tttccaatag acctaacaca
     3361 gctggtaaaa gttgtttctt tctggatacc actccctggg catcgcgagt accatcggag
     3421 agcggcggat aggggaggtc ttccaggatg gtgggcgcat tggccgaaat gagcaggcgg
     3481 ctggtgccgc ggactacatc ggtaacgagg agcatggcaa agtgtaaacc acgtttgtcg
     3541 cgcaggtcat ttaaggcgtt ttgcaggggt tcaaggtgtt cggtcagttg cagaatatcg
     3601 gtgacttctg cttgagccac ggcaaatttg aatccgccgc cttcgaaggg tttgatatct
     3661 gtgctgacca catccttcgg gtcacggttt gaaagccctg cgcctgcgct tagaacagcc
     3721 ttgccatacg attctatggt ttcgcctttg agcgggctcc cgccgacaaa tgcccagcgg
     3781 gataatcggt ctgctgccac tttgtcacgc tgcgtggtcg ttggtgatgt caggatgaga
     3841 gtatcagaac aaagtccagc aagaagcgta cctgccaatg cagggggcat ggagaggccc
     3901 gcttcagcgg tcatttcggc caccaaggtg gaagttgatc ccactgtatc caccgtaaaa
     3961 cggattggtt gatgggtata gggattgcca aggcggtgat ggtcaagaat ttcaagcaat
     4021 tcggcttctt caagtgcggc aatagcctgg cggggttcgt tatgatccac cagaatgatt
     4081 ttcaggcgcg gcgggttaag tacctcgcgc tgattggcaa tgcccacgta tacaccgtgt
     4141 tcgtccacca cccaataatc gttgaattcg tcacgcagga ttttgttgag cgcatcgcgg
     4201 atatgggcgt tggctgcgta tttgggtacg ctggtatctg cggcatcttt gcattgcgcc
     4261 gccatcatct cacggacagt tgtgtcgccg gggcgtgggc caagggtctc gctgaaatat
     4321 ttgaagaggc tcatcccatt gatgatgcca aacggctttc catcttcatt gactacgggt
     4381 gcaacaccgc ccgttttgct ggcaagagtc catgccattc caagctgcga atcagggcgg
     4441 atggtgtcca gtcggcgcat gaccgactcg aagcggggcg atgcatctgt aagtaaaacg
     4501 ggtgtctcaa ggccgactgt tttcagcacc caggaggttt gggcattaag agcgccagcg
     4561 cgtgctgcga ttgtgtttgc accatcgcgt tcgcgcaaga gccaggcata gcccattgca
     4621 gcggcaatgg aatcggtatc ggggttgacg tgtcctatga cataaatttg atcaggcatt
     4681 ttttcttttc caatgaattt agagattaag ttgttgttcc ctgtagttgg ggattattgt
     4741 gggcagattt tgaagaaagt ttcgaagaac gcaaggtaat tatatctcgc cttgttcaac
     4801 cagctttaag aagatattaa cgacccgtgg gtcaaagtgt tttcctgttt gctcgccaaa
     4861 atattttagt gcttgttcac gcggccaggc tttattgtat gggcgatctg atgtgactgc
     4921 atcccaaaca tcggcaacgg caaagatgcg ggctgcgatg ggtatttcct cgcctttcaa
     4981 gccttgcggg tagccacttc catcccattt ttcatgatga gagtacggga tatccagtgc
     5041 cttttttaga aatgggatcg ggctcaagag tttggtggca atttcagggt gtttttttat
     5101 gatctcgcgc tcattatccg tcaatttgtc gggtttgtgg agaatttcat ctggaactgc
     5161 cattttccca atatcatgca aaattgcgcc gcgtcgcatg tgttcaaggt catcaccagc
     5221 aacccccatg gctactgcga ttttcagggt catatctgta acgcggcggg aatgaccttc
     5281 ggtttccttg tctcgtagtt ccaatgcgcg cgcccagcct tccagtgtgg tgtcgtaggc
     5341 atcggccaac tcccaggtac gggcgcggac acggtcttcc agttctgcat tcagtttgcg
     5401 aagttcgcgc tcaagttcct tttgggcggt aatatcatgc gaaatgtaga ttcggcctgt
     5461 catctctttg ttggagttgt agagcggcca gacggttaat tcataataaa catgtacacc
     5521 gcccaggtca aaggtggctt cggcgcgggc ataggaggtt tgcatgtatt gtttaatcgg
     5581 aatggggaaa tccgcgaata tttttttggc tggttccccg atgacatcat cagcagtctt
     5641 gcccagcagg gccagcatgg acaggttgat gtccacaatg tcgttttggt tgttgagaat
     5701 aaccacaggc tcgaccatgg cctcaaatac tgtgtcacgt gcaatggggg tgacgttgaa
     5761 catgcggaac tggaatagcc cccatacaac gaaaaggttg ccaatgacaa aggtgaatgg
     5821 agccatgtct cgctgcggcg tgatgcgtat gtcaaacagg gtcagcgcca tgccgaggat
     5881 gggaatgagc agccccagcg agatcattat catttgagtt tgatacaggc tgtgagaatg
     5941 ggtaatgcgc tgaaacaaaa tacgaagtcc ccatgccaaa atcaaaagtg cgtaaaaggc
     6001 atatccgtaa acgagcggcg tgaaatcgta cagcagttct gtaaaaaaac cctgggtgga
     6061 aaggtgtgga ttcctgtaaa cccaatgcag gttttgatcc agaaacagaa cactggaaaa
     6121 caggatgggg acaataaaag acagcccaaa aagcaggcgt ggataattga gcttgtattc
     6181 tgtgtattgg accagaaata tcggcagcga gatcaaaata aagaatccag caaaccactg
     6241 gaaggaatcc caaaagatct ttgtagagat atcgttggag gttagttcgc agatgaaacc
     6301 tgtcagccag agtgactgcc caagaccgta aaatgagaat gcaaaagccc ctcgagcttt
     6361 tcgcctgctc caggtgtaga cgaagacggc aatcgaaaca gccagcgaga caaggtatgg
     6421 gataaggtga ggaatttcag aacgggtcat gaggatgata gctttattgt atcaacccaa
     6481 tgagtttcat gggtgctttg ttttaccgcg gtttggaaac cctttttaat ataataaaaa
     6541 agcagatgca aatccagcgt gcaatttgca tctgctgctg tttagctggg ggctgatgtt
     6601 tgagccctga gggccttcca aactttttca ggggtgaccg ggttatcgtc cacgtttgca
     6661 ccgatggcat cgagaatggc attgccgaca gcgggtgcga ccccgtccat ggggatttcc
     6721 gccacagcct tcacaccaaa tggatgactc ggctcgaagg tttcgacaaa gatggtttcc
     6781 aactcgggca tttcattcgc gcggaagaga tgatagcggt caaaatcccg ctcgatggca
     6841 tagccctttt cgtcgtaccg catctcttca cagactgcat aaccaagagc ctgcatcata
     6901 ccgccttcga tctgcccgga ggccgttagc ggattcacga tgactccaga atccactgcc
     6961 atcacgagtc gatccactgt aacggcgccc gtttcagtat caacggtcac ttcagcaaat
     7021 tgagcggcaa acggaggcgg cgaaacgggt gagacgtagc ttgccacacc catgatctgt
     7081 tcttgattgt ttttgtgcag tgtgtcgagc gcaatttctg ccagcgaaac ggactttccg
     7141 ttcggcgcgt gtgcgtgacg gttgcgtaat tgaatggcgt tatgatcttc cacacccagc
     7201 atggtcgccg cacgaatctt gatccgctct gctgcgatct gtgcggcttt tgtggcggca
     7261 gtgcctgaga tataggttgt ggaggatgcg taagcgccct tgtcgaatgg ggtgaaatcg
     7321 gtgtcggatg aatagcagat aatgtcttcc acgggcacgc ccagtacttc ggctgccatt
     7381 tgcgccagga ccgtatcgga gccagttccc aggtctgtgg cgccgacgag tagattgaac
     7441 gacccgtcat cattcatctt gatggaggca ccgcccatat caaggtacgg aatcgctgtg
     7501 ccttgcatca ccatcgcaac gccgatgcct tttctctttg aacctgaaac tgagctgcgc
     7561 caaccctgat ttccaaactt gtcatcccag ttgatggcgg cgcggccctg ggctacacat
     7621 tgctcaagac ccacggtgtg aatgatctcg ggtcttggtt cgcggccttc attccatgcg
     7681 gttgagaacg gatgatactc accagggtga attgcattct tgaggcggaa atcgatcggg
     7741 tcgaagccca tggcacgcgc gatcttttcc atgtggcggt cgagcggcca gtagccttgc
     7801 ggcacgccat acccgcggaa tgcgcccgca ggcggtgtgt tggtgtaaac cacatctgca
     7861 taaaaacgga tgttgggcgc tttgcgatat tctccatcgc cgacatacaa agccatcgcc
     7921 ttgtgacctg tgttacctgt cacggtcaag gcatggcagc cgtatgcgcc tgtatctgaa
     7981 agcgcgtaca tcgaattggc tgtgatggtt ccatcttttt tcacgcctgt cttcatgcgc
     8041 acacgcatgg ggtgacgtga acgcgccgcg atgaattcct cttcgcgggt gtattcgtaa
     8101 atgacaggcc gttttgtggc aatggtcaga tgcgcggcta catcttccat caggacttcc
     8161 tgtttgccgc cgaagccgcc gccgattcgc ggcttgataa cgcggatgcg tttgacgggc
     8221 aaacccagta caggtgccaa cattctgcgc acatggaaag gaacctgtgt ggacgtgcga
     8281 attaccaatc ggtcatcctc gtcccaatag gttacacaga catgcggctc aatgtgggct
     8341 tgctgcacct tcggcacttc gtattcggct tcaaaaattt catcggcttc ggcaaagcct
     8401 ttttcaacat cgccgatatc aatgcggata tgtgccgcaa gatttttctc tggattggaa
     8461 tccgcaaagt tgacatactc tagctcgtca tgaatgcgga tcggactccc catggcctgc
     8521 ctcgaatcaa ggatcggagt cagcacctcg tattcgacct cgatcaacga aagagctttt
     8581 tctgcaattt caggtgactc ggcggcgaca aaggccacac ggtcgcccac aaagcggacc
     8641 tttttatcga gtgaaaacgc atccaacggc ccggggatcg ggtctgactg gcctgctgtg
     8701 gaatagacca cgcgcggcag gtctttccat gtcaataccg cggcgacccc ttcgagcgcg
     8761 agggctttgc tggcgtcaat gtttttgatc agcgcgtggg cgtgcggcga gtgtaaaact
     8821 tttgcgtgga gcaatccgcg tttttcaaaa tcggcggcaa aggcaggttt gccttgcacc
     8881 agtttgaccg catctaattt aatttcgggt ttgcccactg tttgccaggt ctgtgtttcg
     8941 ggtgtgacca ctactttggg ccgaacctgc acacccgtca ggtttgaggc gggggcgtca
     9001 ttttgcggag aatcatcttt gggcgttccg aaatcccagt taatggaatt ccaatccagc
     9061 ggcgtatcgc ccctcatttc tgcagcggct ttcatcacgg cttgtacagg tttgagatag
     9121 cccgtacagc gacacaacac acccgaaatg gcttcacgta cttccgcctc acttggattt
     9181 gaattctttt caagcagggc tttcgcagcg aggatttgcg caggggtgca atacccgcat
     9241 tggatcgcgc ctgtttcaac gaaagccttt tgaagcggat gcaaaccatc ggtggtcttc
     9301 cagccttgtt cgggatgttc gccaagtgcc tcgatggtgg caatgtcgtg gccttcggct
     9361 tgtgcggcga gaagcgcccc cgcgttgaca ggtttgccgt tcagcagaac tgtatctgcg
     9421 cctgagaggt tgccctcatc tccaaacttg actccgtgat atcccagccc gcgcaaagcg
     9481 gcaagcaggg tggtggctgg ggcggtatca atggcgtgct caattccgtt gactttgagt
     9541 gtgatgttca tgtgaactcc ttgattcacc gcagacaaaa agatatcttc aatgcatccg
     9601 tatttatctg tggttgaaaa tgtttacaaa acctttgtaa ctttcaagcc agctttcgag
     9661 ccttcgatcg cagcctgtga cccgatcact gcaaccgctt cagatttggc ggctttttca
     9721 agtacctcac caaaagagat gaagtcttcg ccgttggtgg agaggatctc atcacgtttc
     9781 ttttgaagca tggcatcggt tttgcccgtg aggtggcgca tcatggatgt gtaacctttg
     9841 gcgtcgggca gctgataggt atcgaggtcg ccaatggcgg cgatgatggc cttggtgagt
     9901 tcgttatcgt tgaagaggtt cggatccaga ttcttcaagt acccagccgc attgtcgtaa
     9961 ttttcgaggg tggtgtcgag gttggggtcg cggtatgaga ggaaggaaaa tacgccagag
    10021 ttttcgtcga aaacgcagaa tccaccgtaa gccccgccca gtacgcggat tttttcccaa
    10081 aggtaggcca tgcgcaggta gcctgtgacc acatcagcgg agccatcgaa ttcatagcca
    10141 aggtcgtaga ggttggcagc cttgcccacg taattgacct gtgcggggat ggtgaggcct
    10201 tccttttcgg ggtgggcttt gatgtcgaac ggtttgagcg tcacttcctt atcaggcagg
    10261 gcaaagatga agttttcaag gcgcggtttg aaggcggccc aatttgccgc atccagggtg
    10321 acgttgcaga tgagtgacct gctgttgatg agggcgtcgc gcatggcttc cagtttcttc
    10381 aaaaccgacg gccacttctt gtcgatgtct ttggcgagtt cgcgcagggc aaagagatag
    10441 ccgatgccgc tcatctggtc gttgacccag cctgagccgc caaactgtgc gcggacgcgg
    10501 cggttggcga agatatgccc gcctggcacc agtcctgatt ccacgcccga tttttcctcc
    10561 aacacgatct gtttgaggcg ttcacggttg tcgaattttg tggtgagcag aatgtctttc
    10621 aggatggcga gcatctcagg cgcttgactc atcgtggcct tgccgcgaat gaacaacttg
    10681 ctgacgttct ctcttgaccc gtaagctgtg gtgctgaggg cgtcaccgta gattccgcct
    10741 gtgcttttgc cgatgcgttg cgagagtttg acgtaatctt cagtctcggt tcccatttct
    10801 agcagggcgc gggcaaagat ctcggtcagc ggcaggtatt cctgcggcat ggcgtgcagg
    10861 tcaaagccga gatcgagata aacaatgccg ttggtgaaca ggtcatggta tagcacctgc
    10921 gtatcctgca attgcacgat ttcgatgggg atgggtttgt tctctttatc gaggtctttc
    10981 aatttcaaca caggcagggt ggccaccgct tcgggcgcat tgggtgtttc ctgcatgagc
    11041 ttgagttttc tggtgttttc caccatctcg gcgatctgtt ttgcatccag cgattcgcgg
    11101 atctttgcca atcgcgctga ctcttcttct tcgaggcggc gggcaagctc ggggtcgggt
    11161 ttgaggcgca gtgttgtgcg gtgttgattc tccaacaggt gagtctgaat cagcctttcg
    11221 aaatagcgcg ggtcggaggc gagtcgcgct ttgatttcat tgagagtggc ttcgaagcca
    11281 agcaatttga agggatcttc gtcgtcgtgg atccaggtgg tgaggacgcg gcgcatgagc
    11341 gcaatgccgc gcgggaatga gcctgtgtta ttttcgcgca ggcggaattc ggccgtgttg
    11401 actgccgcag tgatcatgtc tgggtcaagg ccatcacgca cgagtgtttc gagggtgtcg
    11461 agaatcaatc tctcgatctt gcgcgcatgg cgggcgcgtg tgcctttcag acccgtggag
    11521 aagatcaggt gacgtaattc ggtttcgagg ccgaggcctg caaggtcttc gccgaggcct
    11581 gaatcgagca gggccttttt gagcggggaa gcaggagtgc cgatcaaaat gctgccgaga
    11641 atgcgcagac tgaagttcaa aacggggtcg gtggtttcgg gcagggtcca attgaccaca
    11701 aggtaattct tggcttcgat atcggtgtcg ctgcctgcgt cgtagctgga ttccactttt
    11761 ttggcgcgct tgaacggctt ggctggcttc acgcttgact tcactttttt gcgcttgtac
    11821 gattttaggt agccttccat caatttcaag cgggtgtcgg ggtcatcatt tccgtaaaag
    11881 aaaatgaacg aattggatgg gtggtaataa ttttcccaaa aggctttgaa atcggcatag
    11941 gtcaggtcgg ggatgttgcg cgggtcaccg cctgaatcga cgctgtagat gtgtttcggg
    12001 aaaagcgatt cgatgatgcg ggtttcaagc acgccttcgg gtgaagagta ggcccccttc
    12061 atttcgttga agaccacacc cttgtaggta agcggctccg caggatcgtt gatctcaaag
    12121 tgccagcctt cctgcatcag ggtttgttca gtgatgagcg gatgcagaac cgcatccaca
    12181 tacacatcga tcaggttgta gaaatcctgc aggttcgggc tggcgatcgg gtaacaggtt
    12241 ttatctggaa atgtgttcgc gttgatgtag gttgccagcg accctttgag caactccaca
    12301 aagggttctt ttactgggta tttctcagaa ccgcccagca cagaatgttc gaggatgtgt
    12361 gccacgcctg ttgagtcttt tggcgtggtg cggaagttga tgctaaatac cttgttctcg
    12421 tcatcattga tgacggaaag caaccgcgca cctgtgcgct tgtgtacgta cagctttgcg
    12481 atggaattta attcgggaac ctgttcttct ttgataaggg taaacgcttt agacattatc
    12541 tctcctgtgt gatagtttac tggtttgaag gtttgaacgt ttaattaacc tggcaacttt
    12601 ccaactttcg aacctgtcaa ctttctggtt ttttctcgta attcgtcgat cgctttcatt
    12661 tttccatgtt ctttgtacaa ccataaatcc cacccattga cggggccgtt tgcaagggcc
    12721 tttgccagag cgtggatcga gccaacctgt tcctgatatt tcaaatgccc atttgacaaa
    12781 atgacggcgc gaatggatgg atcgttctta aagaacaact tttgccccgg tgacaggaat
    12841 cccgcctcaa gcagcgctcc aaacggaacc cgagcctgat tccgtttttc aggcagagtc
    12901 agcgcctcgg caggggattt cgtcactgcg gcgatgcgtt tctgcgcgac ctgaatgtat
    12961 ttcctgtcgc gttcgatgcc gatgaaatca cgtccaagct ttttggcaac tgcgcctgtg
    13021 gtgccgctcc caaaaaatgg gtcgagtacc aggtcacctg ccttggtgct ggaaagcaga
    13081 acccgataca aaagggcctc tggtttttgg gtcgggtgag cctttgtccc gttggatttg
    13141 atgcgctctt tccccgtcgc cagtggaata tgccaatcgc tgcgcatttg caaatcttcg
    13201 ttcagggctt tcatcgcctt gtggttgaag gtgtattttg cgcccttttt cttttgcgcc
    13261 cagatcatgg tttcgtgggc attggtaaaa cgcacaccgc ggaagtttgg cattgggttc
    13321 gatttgagcc agatcacatc gttgaggatc caaaagccaa ggttctgcat gatcgcgccg
    13381 acacggtaga tgttgtgata cgagccgatg acccagatcg ttccattttc cttgagcacc
    13441 ctgcgggcag cgccaagcca ttcggatgtg aattcatcat aggcggcaaa gctggaaaac
    13501 ttgtcccact tgttgttgac cgcgtccact ttgctgaggt tggggcggta aagatcttgc
    13561 tgtagctgca ggttgtatgg ggggtctgcg aataccaaat ccaccgaatt ctcagggagc
    13621 gcgttgagaa cctcgatgca gttgccttgc aaaatctggt taacgggtag cctggtcact
    13681 gctcaaatta tactccgccc gccccatctg gatggatttt tgaggctttt caggctctcc
    13741 ctcgtccaaa attaccattg tggtaaaccc cgatcttgtg gaaaaattca tcatattcca
    13801 tatagaggcc cgccatgaac gaaaaatcca gtgtgtctgc accgatcaac cgactttctc
    13861 cttctgagtt gacagataaa attgcacaag ccaatgtctt tgacctcagc gctttggagc
    13921 ggaatcgcat ggggagactt tctgtcagtc agttttttgt ttctcttgcc gttgtgggaa
    13981 atgccgtttt taatgtggct ggctttttta tgttgaatta ttttattccg gtggggttca
    14041 tgtcgctgcg atccaattca ttcttgaagg ctgctgcctg ctttggagtt tccatcgctg
    14101 tgatcgccat ttttttgtgg aagggaaaga aaggcgcttc cattggattt gaccgttacg
    14161 atatcaagga cattacacaa ttccctctca agagtcttct gattattgac ctgttgcttt
    14221 ggcgggtagc ggaatatcgc gggtctgttt ccagtgataa agcgacgggc atcgttcgca
    14281 caaaggatta tcgcgatcca gataatccaa ccagggtcaa ccctttttct gaggaacaaa
    14341 cggtcaatta ttactcgtac aaagcgggca gggataagtt ccctgtttca tttgaagcgt
    14401 acaaagcttt gcagggaaaa ctgcaagatt gccgccttta ttacctcccc ttaagcaaga
    14461 tcatgatcaa tatggaagtt ttgcagttgt gattcttgac cgtaaatata aaatcggtcg
    14521 cctgttttag attggttaat tttccaaatt atgttcagaa atccctgctt ttgcaatcag
    14581 tacattagga cttcactcat tttgggagtt ttgaaatgca ggacaatata tgatggggaa
    14641 gttgtggcag tgccccctgt tatacttgtc tcatgttgtc acttggtaaa cttggaaagc
    14701 atgaacatgg agtagacatt cattatgccc tatgcatacc gacttgacaa agatgtcaaa
    14761 attttctatc ttgtcacata cggccattgt acttttggtg atattttcag tccctcgctg
    14821 acaccagaac tcaacccggc aaatcggccg agagtaaagt ttattatcga caataccctg
    14881 ggcgatcttg aagtggatat ggagggaatg agatttttca tcaaaagcat ggaagatctg
    14941 aagaagacgg gttttaagct ggagcccacc gctttgttga ccaccaagaa aagcctcgaa
    15001 attttcatta aatcccttga actgcttgtg gatgacgata ttccgaccca cctggcgttt
    15061 gcttcgctaa gtgaagcgct tgtctggttg gatgaagttg aaaataccca ggcgattcag
    15121 cgtatccgtg atgaattgct gggaaagttg cgctcctaga gcgcccaccc gcaaaaaaat
    15181 tcgtcaataa cgcctcagac cactcttcga cgtctcgatc aaactcacaa agatcatcta
    15241 cgccttcgtt ttttcactta taagttcctg ctgtgctttc accaacagag gatgaatgtg
    15301 gggttcataa aagccgctct cccacttgct ctcatcccag agcggaaacc aacgtacttc
    15361 ttttagccga tttattcctc ctgcttggcc ctcaaaacca aggcccaact ctcctccgat
    15421 cggcgtggca agaaacgtca gatatttttg atagtgatat gttcgttcgc gatcttctat
    15481 ttcaaacagt aatcgttcca cgcggacaag aagtcccgtt tcttcacgtg tttctcgaat
    15541 ggcggcttgc tcaaatgtct cgccttcttc cagtccgcct ccaggcagcc accaatagat
    15601 agttcctttt tggtggtttg cgtgacaaat taggagaatt tggccttttt gcaacacgat
    15661 ggtctgacaa cgtgcgatgc gctccatcat ttcacttatc ctccagtgca tggatgatgg
    15721 ctgctataac accgtccata tttttcatta cttcactgtt ccaaaagcgg atgattttgt
    15781 aactgatcga gttcaggtat tttgttcttt cttcgtcata ctcttgctgt tccaaatgct
    15841 gacttccgtc caattcgatg atgagtttct tttcgatgca gacgaaatcg gg
//
LOCUS       DAOQJC010000094        15749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1770_length_15749_cov_9.355168, whole genome shotgun sequence.
ACCESSION   DAOQJC010000094 DAOQJC010000000
VERSION     DAOQJC010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15749
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1770_length_15749_cov_9.355168"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLE39_13630"
     CDS             complement(<1..346)
                     /locus_tag="PLE39_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69420.1"
                     /translation="MLAPEKNWWKPMGRMEKSWLTVALVWCIFLTIMMPLWYFYGKQN
                     VPTETYRTSPADYGAKVNAFVEQYTVGKDEATGFPIVAAPAGSDIYLRASTWQWYPVL
                     QLEKGQEYRLHIS"
     gene            complement(367..519)
                     /locus_tag="PLE39_13635"
     CDS             complement(367..519)
                     /locus_tag="PLE39_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69421.1"
                     /translation="MPENKKEEPQHVGQKIFDNIWLLFVLSLLISTVLYNVWGIIDLM
                     NVPFAK"
     gene            complement(677..1336)
                     /locus_tag="PLE39_13640"
     CDS             complement(677..1336)
                     /locus_tag="PLE39_13640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE69422.1"
                     /translation="MEKIRVLLVDDHALFREGLAAVISSQPNMEVVGNANDGLEAVIK
                     AQELKPDLILMDIQMSTMDGLESTRQIKQILPDTTIVVLTVRDDDEKLFEALKSGAQG
                     YLLKDIRSHKMVEMLQSAMNGEAAITPSLAGRVLAEFRRMSLNQPGSSVADHEDNSLT
                     ERELEVLSLVTKGASDKEVAEKLFVSLNTVKTHIRNILSKLRVNSRKEAAKLAQSKGL
                     L"
     gene            complement(1340..>1915)
                     /locus_tag="PLE39_13645"
     CDS             complement(1340..>1915)
                     /locus_tag="PLE39_13645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE69423.1"
                     /translation="QTLSYLGLMTDQVVDFLSSGKEDAAMERLRRTRETIEKATGDVR
                     NAINSLMDENPISQDLCDQLDMIAKEFSSKGNSAIRWTLNEQASCKCSPEMNEQVIHI
                     VREALTNAVNHAHAAHINMSFSQVDEGYVLKIEDDGGGFDSSQPRPAGHFGIQIMKAR
                     ALRVGGVLTVNSAPRQGTQVSLCFPAEKTGN"
     assembly_gap    1916..2015
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2016..3423)
                     /locus_tag="PLE39_13650"
     CDS             complement(<2016..3423)
                     /locus_tag="PLE39_13650"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNE69424.1"
                     /translation="MVLKSLQGRLGALFIAFVLLVLTSVGATFWGVETQQKDALVINL
                     SGRQRMLIQLMTRLAIEHQDDETKATLAQAETTFEQTLSALENGGETIYLPDDFVTLP
                     QTNDVEIKAALSRVREDWGRFRISMDAIQSMPQGDIELQSAVQDLEAQSALLAQQADR
                     VVQLYEASATTKLNILRNIQIIFLLLALVLLGVGAAVTRQSLLRPLTELGAAAARLGE
                     NDLEYPVQVQGPEEMRNLSQSFDGMRVNLNSARQELIDLNNNLEKRVFQRTQELEALN
                     IVSQEITSRLDIEQVLNSVAEKSRALMDGEVSFLCLLDANQQHLNLQTFSGAADAAIG
                     DTTVISAHDLTTTIVSSEKAVVCSIDTCRDGCSILSQKYRGSHLAASLHSSGQIIGAL
                     CVGSSSPQRFGEESAEMLTKLANTAAIALENARLYEQAERIATLEERRRIAAEMHDGL
                     GQTLSYLGLMTDQVVDFLS"
     gene            complement(3726..4685)
                     /locus_tag="PLE39_13655"
     CDS             complement(3726..4685)
                     /locus_tag="PLE39_13655"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE69425.1"
                     /translation="MNTGLIWLGRVVGLIVALALVGGIYEAVAEAADAKAYPPPGQLV
                     DVGGYRLHINCTGTGGPTVVIDAGLGDWSTGWAWVQTDVSQETRVCTYDRAGYGWSDE
                     GPLPRNAEQFAKELHTLLHNANIPGPYVMVGHSLGGLTARVFTGMYPAEVAGVVLIDS
                     MSPRQFKQSSGNPVTKSDLQSRPFVPFTLLARIGLVRLLAGPLGLIPNLPSDAQKMYL
                     AKMSRATYLQTYFGDESRGMLESRGQAEAVTSFGDLPLIVLTAKLNDMSGWQDWQTEL
                     LQLSSNSQQLFAESGHTIEIDKPDAAVTAILKMVERVRLSSRK"
     gene            complement(4783..5160)
                     /locus_tag="PLE39_13660"
     CDS             complement(4783..5160)
                     /locus_tag="PLE39_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69426.1"
                     /translation="MPYTVIYNSELHVVEAKLQGDMTVGEVEEIITRIAQTAKEKGCR
                     SIFTDFREVKQKLSILEIYKLPDRTRNIFASFGFDVSLFRRANIAADDLDDYIFHENV
                     MVNRGQNEKVFTDIHNARKWLIG"
     gene            complement(5165..5887)
                     /locus_tag="PLE39_13665"
     CDS             complement(5165..5887)
                     /locus_tag="PLE39_13665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69427.1"
                     /translation="MSFATFWAEDTLTSIASLPGFTAGPAFDWDELAGINRISQAEVM
                     DRVNHGHRPYVARMDGQPVAYGWLATSKISIGELGINVELPSGDRYLWDFATLPDWQG
                     RGFYPRLLQSILEQEIQNAKRFWIIHAPENLPSGAGMSKAGFEFVGQLSFTWDGKIGL
                     APYADSTERARIGADLLQVPLIESVLAPCWSCGGMVAKHPDQVDADSCWPPQHASENA
                     CCCVKEVKRSNSGITLSAHSAR"
     gene            complement(5957..6949)
                     /locus_tag="PLE39_13670"
     CDS             complement(5957..6949)
                     /locus_tag="PLE39_13670"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNE69428.1"
                     /translation="METKANTAKMKAVVNTQYGSPDVLQLKEVERPTPKDNEVLVKVH
                     AVSVNAADWHLLRADPFLVRLQFGLFKPRHTILGADIAGRIEAVGRDVKRFKVGDDVF
                     GDISGSGWGGFAEYACAREDTLVRKPANLSFEEAAAVPMAAVTALQGLRKGGIQPGKK
                     VAINGASGGVGTFALQIAKAFGAEVTAVCSTRNVELARSLGADHVIDYTQEDFTKNGK
                     QYDLILATNGYHPLADYQRALSPTGSYVMTGGTNAQMTEAMLYGPLKSRNGKQQMGNL
                     LAQPNQKDLETVAELLAGGKVKSIVARRYSLAETADAIRCVEAGHAQGKIVITT"
     gene            complement(7001..8185)
                     /locus_tag="PLE39_13675"
     CDS             complement(7001..8185)
                     /locus_tag="PLE39_13675"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNE69429.1"
                     /translation="MNIILEGNGNTLLTGPVIDQSALHGLLKKVRDLGMTLLSVNGVH
                     LDQAEAPEASQNFLLSNQNNLGGILMKAIVYTKFGSPDVLKLQEVAKPAPKDNEVLIR
                     IVATTVAVEDPGIRARPGLNGFLKPKRSILGFYLAGEVEAVGKDVKRFKKGDKVYGNT
                     GLSLLGTYAEYICMPEDGALAMKPANITDEEAAALPNGALTALPFLRDKGNIKSGQKV
                     LIYGASGAVGTIAVQLAKYFGADVTGVCSTMNLELVKSLGADKVIDYTQEDFTKNGQT
                     YDIIFDTVGKSAFSRCKDSLTENGIYLTTVPSLATFIQMLRTSMTGGKKVGIAATGLR
                     PSNEKTKELIFLNGLIEAGKIKAVIDRSYPLEQIAEAHKYVEAGHKKGNVVITLNHNA
                     KN"
     gene            complement(8514..11228)
                     /locus_tag="PLE39_13680"
     CDS             complement(8514..11228)
                     /locus_tag="PLE39_13680"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNE69430.1"
                     /translation="MSTPVLATKLSIPVPRPKIVPRPHLTDQLNVGLHRKLTLVSAPA
                     GFGKTTLVAEWIASCERPVAWLSLDEADNDPIRFLTYLIAALQTIVSTIGADVLRILQ
                     SAQPQSSEAVLTILINEIAAISGDFILVLDDYHLIEEKTIGNALTFLLEHMPPQMHLV
                     IITREDPNMPLARLRVRDQLTGLRATDLRFTDSEAAEFLNQVMGLRLSAEDIAALEAR
                     TEGWIAGLQLAAISMQGHKDAGGFIKSFTGSNQFVLDYLIEEVLHQQPESIQAFLLRT
                     SILDRMCGSLCDAVLLDSSTSGQETLESLEHSNLFIVPLDNERHWYRYHHLFGSLLRQ
                     RLGQSWKPEEIARSHIRASEWFEKHEDPAQAFHHAIAAGDHSRAAALAEKYWQGMNEN
                     FQSAAWLGWVKQLPERLIRSRPVLCTQIAWVYMDLSEAEASESRLRDAEQCLENPTDE
                     MVVVAEDQFRSLPARIAIARTYNAQSVSDYPAALKYAKLAIKLAPEEDHFLRAQTNAI
                     LGGTYWANGDLDAACKSMSDWIDNAQKAGNYIFAIASASGKADILMEQGYLREALRTY
                     QQSLQLASEHETESQHIIAHHHLGLALLYHEMQNDESAAQHFQKSMELGQYSVLQDWS
                     YRKNLAQARLKESEGDLDSALGFLDDAKRFYTRSLIPNTRPIDAFKARIFLKQGRLSN
                     AQEWVRQHGLSVSDDPGYLREFELGTLARVLLSEYQTHRKERIINDALNLLKRLLKAA
                     EDQKRMGSILEILMVTALAYHALGSTSQAFVTLERALTLARPEGYVRIFVNEGDLMRA
                     LLTDFRASNEKQRRGNGSDLKDYVDKLLSAFTQPKDVQRSKLIEPLSKRELEILQLIA
                     QGLSNQEIGKRLFLALNTVKGHNRIIFDKLQVQNRTEAVARARELDLL"
     gene            complement(11383..11604)
                     /locus_tag="PLE39_13685"
     CDS             complement(11383..11604)
                     /locus_tag="PLE39_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69431.1"
                     /translation="MGETSSTKTGTFEPVGPVPEHQRKGLARVLMAEGLKRLQKLGAV
                     MVYVSSYGKAAHATYESVGNHLELWEKSF"
     gene            11797..13626
                     /locus_tag="PLE39_13690"
     CDS             11797..13626
                     /locus_tag="PLE39_13690"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69432.1"
                     /translation="MYRKFLSILFLTVLLTGCMTPPSVTETILVPEVSETAVVTSPVL
                     QLLNTSFILTSTDVSNGGVLPVEFTCDGNGSTLPLAWSGAPSETQSYAVIMHHEAPDG
                     IHWYWVLYNIPADVTSLPKNVSGIGTLGNNINNGLVEYSPPCSKGPGDKIYTYTVYAL
                     AAEPQINVPAEQVNRDTLLAAIQNITLASAELNVVYAREGMTDPNLQGNQQEQGNNQQ
                     SNNQLTNIAPAYNIEQATSDKAQGMTISYDALAFMTGDLGSDSFFPPGKVADFWGFQY
                     LRDNDPSQMGHAGDFLTSAAMNTLNNLTANQRAQLVALAQSQVDDINTYGYKRFILMD
                     AFRRLAEGDLPAGTTGLNEEAVKAYSAELYQLDGQISYERAQLMGQILISLSAEQRAY
                     FDAMVGKGMLEWNDVQEPEDVRGLDRDTKVAVMTYAADLFSWYAGSVEADVYFCPERH
                     GTYFGSFYLKDIKAMSDPTYAIPTNLTGDLGETMLQKLTPDQAALITGLVDNQKSALQ
                     GMVDAREQISIELRKFKDGSAADQATIMSLMEQYGAYDGEIIYNMAVNFAAVKSSLTS
                     EQQAELDALRTELLGDQSHPTGAYLYSQAIPMPEIPNSDFLFK"
     gene            13669..15486
                     /locus_tag="PLE39_13695"
     CDS             13669..15486
                     /locus_tag="PLE39_13695"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNE69433.1"
                     /translation="MYKKIFSLLLICSLLTTGFTFASTAPQPASAQAATATFEDVPTT
                     YWAYQWIEALAANSITGGCSTTPKLFCPDTLVTRAQMAVFLLRSKHGNAYTPPAATGT
                     VFTDVPAGYWAADWIEQLSAEGITSGCSTGLYCPETIVSRDQMAIFLLRSEYGSSYIP
                     PAATGTVFTDISADYWAASWIEQLVNEGITAGCSDTGYCPEQSVTRAQMAVFLVRAFN
                     LPPTPPAFNLDQTLSDGAQLNTIAFDGLAFITGSLGADSFFPPGKVADFWGFQYLRDN
                     DPSEMGHNTDFLTRASLNLLYVLTSDQKAALITLAKNQVASINEYGYKRFVLMKAFRR
                     LVEGDLPAGTTGLNPDAVKAYSAELYQLDGQISYERAQIMGGIISAFTTEQRAYLDAM
                     VGQGMTSWPVVNEPSELKDLTQDEKIAVMTYAGDMFSWYAGSFQADVYFCPERQGTYF
                     GSFYMKDAPAMGNPDFTIPSNLTAEMGDAFLKTLTADQAALINNLVTDQKPALYGIVS
                     SREEVSTMLRQFMAGGTPDQTAILRLMETYGELDGEIITSYIFAFNQINQTLTASQRA
                     QLLKLRVDWLQDLNQPTGAFLYSQAITMPEIPNTDFLFK"
     gene            15557..>15749
                     /locus_tag="PLE39_13700"
     CDS             15557..>15749
                     /locus_tag="PLE39_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69434.1"
                     /translation="MKKLLFTLLFLSLLAACIPATESPPQTQTTSAAATDSSFMQSVQ
                     PSSLPLPTETNKPKGHNQPV"
BASE COUNT         3837 a         4310 c         3595 g         3907 t
ORIGIN      
        1 aggaaatgtg caggcggtat tcctgtccct tttcaagctg caagacggga taccactgcc
       61 aggtgctggc acgcagatag atatctgaac cagcaggcgc ggcgacaatc gggaaacccg
      121 tcgcttcatc cttgccgacg gtgtattgct ccacaaaggc attgaccttc gccccgtagt
      181 ctgctggtga ggttcggtag gtttctgtgg gcacattttg cttgccgtaa aaataccaga
      241 gcggcatcat gatcgtcaga aaaatgcacc agaccagggc aaccgtgagc caggattttt
      301 ccatccgccc catgggcttc caccaatttt tttcaggcgc taacatagtc tattcctcca
      361 acccttttat ttggcaaacg ggacgttcat caagtcaatg attccccaca cgttgtaaag
      421 cacggtgctg atcaacagcg acaacacgaa cagcagccag atgttgtcaa aaattttttg
      481 acccacatgc tgcggctctt cttttttatt ttcgggcatg gcaattctcc tcatgatgtg
      541 attgaaaaat gcagaatggc ataacccata ttactgtaat tgccccgata aacgaattgt
      601 tatatgtaat cattgcaata aaaaaagagt gatttttaca tcactctttt ttgaaaaaga
      661 attagttctt tttcaactaa agcaaccctt tgctttgagc cagcttcgca gcctcctttc
      721 tggaattgac acgaagcttt gaaagaatat tgcggatgtg agtcttgacc gtgtttaggc
      781 tcacaaataa tttttcagcc acctctttgt ctgaagcgcc tttcgtaaca agggaaagca
      841 cttccagttc ccgctcggtc aaagagttgt cttcatgatc tgccacggaa ctgcctggct
      901 gattcaagct catcctgcga aactcggcaa ggacccgccc cgccagcgac ggtgtaatag
      961 cagcctcacc gttcatggcg ctttgcaaca tctccaccat tttatgcgag cggatatcct
     1021 ttagaaggta cccctgcgcg ccgctcttca acgcttcaaa taatttctcg tcatcatcgc
     1081 ggacagtgag cacaacgatc gtggtatcag gcaggatctg tttgatctgg cgggtggatt
     1141 ccaaaccatc catggttgac atttgaatat ccatcaagat caggtctggt ttaagctcct
     1201 gtgctttgat caccgcctcc aatccatcgt tggcatttcc gaccacttcc atattgggct
     1261 gtgaactgat caccgctgcc agcccttcac gaaacaaggc atggtcatcc accaataaca
     1321 cgcgtatttt ttccataaac taattccctg ttttttctgc ggggaagcac aagctgactt
     1381 gtgtaccctg cctgggcgcg gagttgaccg tcaaaactcc gccgactcgc aatgcacgcg
     1441 ctttcatgat ctgtatgcca aagtgtcctg cagggcgggg ttgggaagag tcgaaaccgc
     1501 ccccatcatc ttcgattttc aatacatacc cctcgtcaac ctggctgaaa ctcatgttga
     1561 tatgagccgc atgggcgtga ttgaccgcat tggtcagcgc ctcgcggaca atatgaatga
     1621 cctgctcatt catttccggt gaacatttgc aggaagcctg ctcgttcaat gtccaccgaa
     1681 tcgccgaatt ccctttggaa gaaaattcct ttgcgatcat gtcaagttga tcgcaaagat
     1741 cctggctgat cgggttttca tccatcaggc tattgatcgc gttccgaaca tcgcctgtgg
     1801 ccttctcaat ggtttcgcga gtgcggcgca gcctctccat tgcggcatct tccttgccac
     1861 tggaaagaaa atccaccacc tgatcggtca tcaacccgag gtaactcaag gtctgnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntggaa agaaaatcca ccacctgatc
     2041 ggtcatcaac ccgaggtaac tcaaggtctg ccccagccca tcgtgcatct ccgccgcaat
     2101 acggcggcgc tcttccagtg ttgcaattcg ttccgcctgt tcatacagcc gtgcattttc
     2161 caatgcgatc gcggcggtgt ttgcaagttt cgtcagcatt tccgccgact cttctccgaa
     2221 acgctgcgga cttgaactgc ccacacacaa tgcaccgatg atctgcccac tggaatgcaa
     2281 ggaagctgcc agatgactcc cccgatattt ttgtgagagg atcgagcacc cgtcacggca
     2341 ggtatcaatg ctgcaaacca cagccttctc gctgctcaca atggtggttg tcagatcgtg
     2401 ggcactgata accgtcgtat ccccgatagc cgcatccgct gcaccactga acgtttgaag
     2461 attaagatgc tgctggttgg catccagcaa acataaaaaa gagacttcac catccatcag
     2521 cgcgcgtgat ttttcagcca ctgaattcaa cacctgttca atatcaagcc gcgaggtgat
     2581 ctcctggctg acgatattca acgcttccaa ttcctgtgtc cgttgaaaga cacgcttctc
     2641 caaattatta ttgaggtcga tcaattcctg gcgggcggaa tttaagttga cgcgcatccc
     2701 atcgaaggat tgagacaagt tccgcatctc ttcgggacct tgaacctgca ccgggtattc
     2761 gaggtcattt tctccaagac gggcagccgc agcgcccaac tccgtcaacg gccgaagcag
     2821 ggattgacgc gtgaccgccg cccccacccc aagcaacacc aacgccagaa gcaaaaagat
     2881 gatctgaata tttcggagga tgttcagttt tgttgtggca gaggcttcat ataattgcac
     2941 caccctgtct gcctgttgtg ccagcaaggc agattgcgcc tccagatcct gaacggcaga
     3001 ttgcagttca atatcaccct gcggcatgga ttggatcgca tccatggata tgcggaaccg
     3061 cccccaatct tcccgcacac ggctcaatgc cgccttgatc tctacatcgt tcgtctgagg
     3121 cagggtcaca aaatcgtctg gcagatagat ggtctcgccg ccgttttcca atgctgaaag
     3181 tgtttgctca aatgtcgttt ccgcctgcgc cagcgttgcc tttgtctcat catcctgatg
     3241 ctcgatcgcc agccttgtca ttaattggat caacatcctc tgccgccctg agaggttgat
     3301 cacaagcgca tccttttgct gggtctcgac accccaaaat gttgcgccaa ccgaagtaag
     3361 cacaagcaaa acaaatgcaa tgaataaagc acctaaccga ccttgcagac tcttaagcac
     3421 catccacctt tttgaaacag gttgattttg attttggaat tatacaccgg agggtattgc
     3481 cgaactggaa gccgaaaaga tgagggtctc tcaagttatc ctatagaatc ttcgccgcaa
     3541 aaaacaaaaa cgcgactcgg aacgacactc ttttggactt gttatctcga agtggctact
     3601 ccctggttaa tgatggggta aaacaaaagg attatcgatc aaactgtttt tcatacaccc
     3661 caaaaccgaa tccgcccgcc ctgtatcatg acaaactctt accatactga ccctgccgca
     3721 atcatctact tccggcttga caggcgtacc cgttcaacca ttttcaaaat cgctgtgaca
     3781 gccgcatcgg gcttgtcaat ttcgatggta tgaccactct ctgcaaaaag ttgctggctg
     3841 ttggacgaca attgcagcaa ttcggtctgc caatcctgcc aacctgacat gtcattaagt
     3901 ttggcggtca gaacgatcag aggcagatcg ccaaaagatg taacggcttc cgcttgcccc
     3961 ctgctttcca acattccacg agactcatca ccaaaatagg tctgtaaata tgttgcgcga
     4021 gacattttcg ccaggtacat tttttgcgca tccgacggca ggtttggaat tagcccgagc
     4081 ggtcctgcaa gcaagcgcac aagcccaatg cgagccaaca gcgtgaaagg cacaaatgga
     4141 cgcgactgca ggtcactctt cgttacgggg ttcccagaag attgcttgaa ctgcctcgga
     4201 ctcatcgagt caatcagcac gaccccggcg acctccgctg gatacatgcc ggtaaacacc
     4261 cgtgcagtga ggccgccaag cgagtgtccc accatcacat atgggccagg gatattggca
     4321 ttatgcaata aggtatgcaa ttcttttgcg aactgctccg cgttgcgtgg cagaggtccc
     4381 tcatcactcc aaccatatcc agcccgatcg taggtgcaaa ctcgtgtctc ctgcgagaca
     4441 tctgtctgta cccaggccca ccccgtagac caatcaccca gaccagcgtc aatgaccaca
     4501 gtcgggccac ctgtaccggt gcagttgata tgcaagcgat aaccgccgac atccaccaac
     4561 tgaccgggag gcggatatgc cttggcatca gcagcctctg ccacagcctc gtaaatgccc
     4621 cccaccaatg ccaatgctac gatcaacccc acgacccgcc ccagccagat caaacctgtg
     4681 ttcatttgtt tttccttttg gttgatattg ctttcgcaat atcttctcca agggttagat
     4741 ccaccccgga gggaacatcg ctggccttgc caggcggcac gatcatccaa taagccattt
     4801 ccttgcgttg tgaatatcag taaatacctt ttcgttttgt ccgcgattga ccatgacatt
     4861 ttcgtggaaa atataatcat ccaggtcatc tgctgcgata tttgcccgtc gaaagagcga
     4921 tacatcaaag ccgaatgagg cgaagatgtt tctggtgcga tcaggcaatt tataaatttc
     4981 caggatcgat aacttttgct tcacttcacg aaaatcagtg aaaatggatc ggcaaccctt
     5041 ctctttggct gtttgcgcga tcctggtgat aatttcctcc acttcgccca cagtcatatc
     5101 cccttgcagt tttgcttcaa ccacatgtaa ttctgaattg tagataaccg tataaggcat
     5161 gatttcacct cgccgaatgc gcagagaggg tgataccgct attcgatctt ttgacctctt
     5221 tcacacagca acatgcgttc tcactggcat gttgaggcgg ccagcatgaa tcagcatcaa
     5281 cctggtcagg atgttttgcc accattcctc cgcaactcca gcatggagca aggaccgact
     5341 caatgagggg aacctgtaac aggtccgcac cgatgcgggc gcgttcagtt gaatctgcat
     5401 agggggcaag gcctattttt ccatcccatg tgaaagagag ctgcccgaca aattcaaatc
     5461 ctgccttgct catacccgca ccggaaggga ggttttccgg tgcatggatg atccagaagc
     5521 gtttggcatt ttggatctcc tgttcgagga tggactgtaa caaacgcgga taaaacccgc
     5581 gtccctgcca atctgggaga gtggcgaaat cccagagata gcgatcacct gatggaagtt
     5641 caacgttaat tccgagttcg ccgatggata tcttgcttgt ggcaagccag ccatatgcaa
     5701 ccggttgacc atccatccgc gcaacgtatg ggcgatgccc atgattgaca cggtccatga
     5761 cttctgcctg agagatgcga ttaatcccgg cgagttcatc ccaatcgaag gcagggccag
     5821 ctgtaaatcc aggtaatgat gcgattgagg tcagggtatc ttcagcccaa aaggtggcga
     5881 atgacatttt gtctcctcgt aaaagtgcta ccaataaatt caagaacacg tttcagagtt
     5941 ggaatgaaaa tcagatctac gttgtgatca cgatcttgcc ctgagcgtgg cccgcttcca
     6001 cgcagcggat ggcatcggca gtttccgcca gcgaataccg cctggcaacg atagatttca
     6061 ctttgccgcc cgccagcagt tcagcgacag tttccaaatc tttttggttc ggctgcgcca
     6121 acaggttgcc catttgttgt tttccatttc ttgatttcaa cggtccatag agcatggctt
     6181 ctgtcatctg cgcattggtg ccgcccgtca tcacatagct gccagttggg gataaggcgc
     6241 gttggtaatc agccaacgga tgatagccat tcgtggcaag gatcaggtcg tattgctttc
     6301 cattttttgt gaagtcttcc tgggtgtaat caatgacatg gtctgcacca agcgaacgag
     6361 ccagttcgac attgcgggta ctgcacactg cggtgacctc tgccccgaag gctttggcga
     6421 tctgcaaagc gaatgtgcca acgcctccgg acgccccgtt gattgccact tttttcccag
     6481 gctggatgcc gccctttcgc agtccctgca acgctgtgac cgccgccatc ggcacagccg
     6541 ctgcttcttc aaacgagaga ttggcaggtt ttcgcacgag agtatcttca cgggcacaag
     6601 cgtactctgc gaagccaccc cagccagaac cagagatatc gccaaataca tcatcaccga
     6661 ccttgaagcg tttgacatcc ctgcctacgg cttcgatgcg cccggcaatg tcagcgccaa
     6721 ggatcgtgtg cctgggtttg aaaagcccaa actgcaaacg gacaagaaat gggtcggcgc
     6781 gcaagagatg ccagtcggca gcgttgacag acacagcatg gacctttacc aaaacttcat
     6841 tgtccttggg cgtggggcgt tcgacctctt tgagttgtag aacgtcgggt gatccgtatt
     6901 gggtgttcac gaccgctttc attttggctg tgtttgcttt tgtttccatg atatttgctc
     6961 ctgtgatcga attcatcttt atcttcttat tctctgtaat ttagtttttg gcattatggt
     7021 tcagggtgat gacgacattt ccctttttat gccctgcctc aacatacttg tgagcttcgg
     7081 cgatctgctc cagcggatag cttcggtcaa tgaccgcttt tattttgcct gcctcgatca
     7141 gtccgttgag gaaaatcaat tccttcgtct tttcgttgga aggtctcaac ccggtcgccg
     7201 cgatccccac cttctttccg cctgtcatgg atgtacgcag catttggatg aaggttgcca
     7261 gtgacggaac tgtggttaga tagatcccat tttcggtcag cgagtcctta cagcgtgaaa
     7321 acgcactctt gcccactgtg tcaaaaatga tgtcgtacgt ctgaccattc tttgtaaaat
     7381 cttcctgggt gtaatcaatg accttgtcag ctcccaacga tttcaccaat tccagattca
     7441 tggtgctgca taccccagtg acatccgcgc caaagtattt ggcgagttgt acggcaatgg
     7501 ttcccaccgc cccggatgct ccatagataa gaactttttg tccgctctta atatttccct
     7561 tgtctctcag gaagggcaag gcggttaatg ccccgttggg aagggcagcg gcttcctcat
     7621 cggtgatgtt ggctggtttc attgcaagcg ccccatcttc aggcatgcaa atgtattcgg
     7681 cataggtacc caataggctc agcccggtat ttccatacac tttgtcgccc ttcttgaatc
     7741 gttttacgtc ttttccgacg gcttccactt cgccagccag ataaaatccc aggatggatc
     7801 tcttgggttt caaaaacccg ttcagacctg gacgggcgcg tatacctggg tcttctactg
     7861 caacggttgt tgcaacgatt cttatcagta cttcattgtc cttgggggca ggcttggcta
     7921 cctcctgtaa tttaagaacg tctggtgatc cgaatttggt gtacacaatt gctttcataa
     7981 gtattcctcc gaggttattt tgatttgaca ataagaaatt ttggctggcc tcaggcgctt
     8041 ccgcctgatc gagatggacg ccgtttaccg atagcaaggt catgcccaga tcgcgtactt
     8101 tctttagcaa tccatgtaat gcggactgat cgatcaccgg gcccgttaac agcgtgttgc
     8161 cgttgccttc aaggatgatg ttcatgccct caaaccaatc tacccattga tcgttcaggt
     8221 gacccttaat cctgatttga taaaccattg gctgtcctgg gtcggttttc aggttttgtc
     8281 cattcgtcat tccgttgttc cttttgtggc cgtttgtttc agtccgcacc acttattcag
     8341 tgtagggtta gtttacctac aaggaggtgt taacgaatag acacttaagt gttattgggg
     8401 gtgttgtttt tggatggggt gtgacggaag gggtattaaa gccacgaaca aaatccccct
     8461 tcgtgcgtgg ctcagcacct aaaatcagat ttatcgatca agtaaccgtg cagctataac
     8521 agatccaatt cacgggcacg cgccacggct tcggtgcgat tttggacctg taacttgtca
     8581 aagataattc gattgtgccc ttttacggtg ttcaaggcaa ggaaaagccg cttgccgatc
     8641 tcttgatttg agagaccctg ggcaatgagt tgcaaaattt ccagctcacg cttgctgagc
     8701 ggctcgatca gttttgatcg ttgtacatct ttgggttgtg tgaaggcaga gaggagttta
     8761 tctacatagt ctttcaagtc gctgccattg ccgcgccgtt gcttttcatt ggaggctcta
     8821 aaatccgtaa gcaatgcccg catgagatcg ccttcattta caaagatgcg gacatagcct
     8881 tccggtcttg ccagggtcag agcgcgttcc agcgtaacga atgcctgaga tgtgctgcca
     8941 agtgcatggt aggcaagagc cgttaccatc aaaatttcga gaatgctccc catccttttt
     9001 tggtcttctg ccgccttcaa cagccgcttc aacaggttca atgcgtcatt aataatgcgt
     9061 tcttttcgat gggtttgata ctcagacaga agcacccgcg ccagggtacc aagctcgaac
     9121 tcgcgcaaat agccaggatc atcgctcacc gaaagcccat gctggcgcac ccattcctgc
     9181 gcattggaca atcgtccttg tttgagaaaa atcctcgcct tgaatgcatc aatgggacgt
     9241 gtgttgggta tgagagacct ggtgtaaaag cgtttggcat catccaaaaa gccgagtgca
     9301 gagtccagat ctccctcaga ctctttcagc cgggcttgag caaggttttt acgatatgac
     9361 caatcttgca aaacggaata ctgaccgagc tccatgctct tctgaaaatg ctgcgcggca
     9421 gattcatcgt tttgcatttc atggtaaaga agtgccaacc ccaaatgatg atgcgcaatg
     9481 atgtgttgcg actcggtttc atgctctgaa gcaagctgca gggattgttg ataggttctt
     9541 aatgcttcac ggagatatcc ttgctccatc agaatatccg cttttccaga agcgctggca
     9601 atggcaaaaa tatagttacc ggctttctgc gcattatcga tccaatcgct catggatttg
     9661 caggcggcat ccagatcgcc atttgcccaa tacgtgccgc ccaaaatcgc attggtctgg
     9721 gcacgcaaaa aatgatcttc ttcaggagcg agtttgatcg ccagttttgc atacttcaac
     9781 gcggccggat aatcgcttac agattgggca ttgtatgtgc gggcgatcgc gatccgcgca
     9841 ggcagggaac gaaattgatc ttccgccaca acaaccattt catccgttgg attttccaga
     9901 cactgttcag catctctgag gcgggactca ctggcttccg cctcactaag atccatatac
     9961 acccaggcga tctgtgtgca aagtacggga cgcgaacgga tcaatcgctc cggtagctgc
    10021 ttcacccaac ccagccaggc agccgactga aagttttcat tcataccctg ccaatatttt
    10081 tcagccaacg ccgccgccct gctgtgatcc ccggcagcga tggcgtggtg gaatgcctga
    10141 gccgggtctt cgtgcttctc aaaccactca ctggcgcgga tatgagatcg ggcaatttct
    10201 tcgggcttcc aactttgtcc caaccgctgg cgtaatagac ttccaaagag gtgatgataa
    10261 cgataccaat gtctctcgtt atccagcgga acaatgaaca ggttgctgtg ttcgaggctt
    10321 tccaacgtct cctgcccaga tgttgaagaa tccagtaaga cggcatcgca aagggaaccg
    10381 cacatgcggt cgagaatgga tgtgcgcagc aaaaaagcct gaatgctttc aggctgttga
    10441 tgcaaaactt cttcgatcag atagtccagg acaaattggt tgctgccggt aaaagacttg
    10501 atgaatcccc cagcatcttt atgcccttgc attgagatcg ccgccaattg caatcccgca
    10561 atccagcctt ccgttcgggc ctccagcgca gcgatgtctt ccgccgaaag gcgtaatccc
    10621 atcacctgat tgaggaattc ggcagcttca gagtctgtaa aacgcaggtc tgtggctcgc
    10681 agaccggtca attgatcgcg aacacgcaac cgggccaaag gcatatttgg atcctcacga
    10741 gtgatgatca ccaaatgcat ttgtggcggc atgtgctcca gcaagaaggt tagcgcatta
    10801 ccaatcgttt tttcttcaat cagatggtag tcatccagga caaggataaa atcacccgag
    10861 atggcggcaa tttcattgat caaaatagtc agcaccgctt cggatgactg tggttgagca
    10921 gactgaagta tacgcagcac atctgcccca atggttgaaa caatcgtctg cagagcagcg
    10981 atcagatacg tcagaaatcg tatggggtca ttatccgctt catccaacga caaccaagcg
    11041 acaggtcttt cacaactcgc gatccattcc gccaccaggg tggtttttcc aaagcctgca
    11101 ggagcagaaa caagggtcaa tttacggtgc agacctacat tcagttgatc agtgaggtgg
    11161 ggacgaggga caattttagg ccgtggtaca gggatggaaa gtttcgtggc caaaactggt
    11221 gtggacatgg attcattata aaaccaatat tcaaaaatta acaccttccg agatcattga
    11281 gatcaccgtt cgggtaatct tccatgggcg gaatgtaatt gggtgtttta tttcttcata
    11341 aaatcgaaag agataccaac aaacaaaccc agaagtcaat tcctaaaaac tcttctccca
    11401 caactccaaa tgattcccaa ctgactcata agttgcatgt gcggctttgc catatgagct
    11461 gacatatacc atgaccgcac cgagtttttg cagtcgtttc aacccttcgg ccattaatac
    11521 tctcgcaagt cctttgcgct ggtgttcagg gaccgggcca acgggctcga aggttccagt
    11581 tttagtggaa cttgtctcac ccatgttgag taaaactccc aaatttataa gggaaatcca
    11641 gcggctttgg acggtttaat catttttgag atgacttgta tttattttac agtctctcaa
    11701 aaacccttga aaggatggcg taaacaaaat cctaaaccct tctaaacaat ttccttacat
    11761 cttcctgcta tcgtaggagt gaaaggaaaa aatgatatgt accgcaaatt tctctccatc
    11821 ctctttctca ccgttttgct aacaggatgc atgacaccac cctcggttac agaaaccatt
    11881 cttgttcctg aagttagtga aactgctgtt gtcacgtctc ctgttttgca gttactcaac
    11941 acaagcttca tcctcaccag tacagatgtt tcgaacggcg gcgtactgcc tgtcgaattc
    12001 acctgtgacg gcaacggttc gactctcccg ctcgcctgga gcggagcccc cagcgagaca
    12061 caaagctacg cagtcatcat gcatcatgaa gcacccgatg gtattcattg gtattgggtg
    12121 ttgtataaca tccccgccga tgtgaccagc ctacccaaga atgtaagcgg tatcggcaca
    12181 cttggcaaca acatcaacaa tgggcttgtc gagtattccc cgccctgctc aaagggaccc
    12241 ggtgacaaga tatacacata caccgtgtac gcgctcgccg ccgaaccaca gatcaacgtt
    12301 cctgctgaac aggtgaatcg tgacaccttg ctcgcagcca tacaaaatat cacccttgcc
    12361 agcgctgaac tgaatgtagt gtatgcccgc gaaggtatga cggacccgaa tttacagggg
    12421 aatcaacagg aacagggtaa caaccaacaa agcaacaatc aactcaccaa tattgcaccc
    12481 gcctacaata tcgagcaagc cacctcagat aaagctcagg gcatgaccat ctcttacgat
    12541 gccctggcat tcatgacggg cgatctcggc tcagactctt tcttccctcc tggcaaagtt
    12601 gcggacttct ggggcttcca atatctgcgc gacaatgacc cttcccaaat gggacatgcg
    12661 ggcgacttcc tcaccagtgc cgcgatgaac acactcaata atctcactgc caatcaacgc
    12721 gctcaactcg ttgctcttgc tcaaagccag gtggatgaca tcaacaccta cggatacaaa
    12781 cgcttcatcc tcatggacgc cttccgccgc ctcgccgagg gtgacctccc cgctggcacg
    12841 actggactca atgaagaggc ggtcaaagcc tactctgcgg agttgtatca gctcgatgga
    12901 cagatcagct acgaacgcgc ccaattaatg ggacagatcc tcatcagcct tagcgcggaa
    12961 cagcgcgcct acttcgatgc gatggtcggc aaaggcatgt tggagtggaa cgatgttcag
    13021 gaacccgaag atgtgcgtgg acttgaccgc gataccaaag tcgctgtgat gacatacgct
    13081 gccgatctgt tcagttggta tgctggctct gtcgaagcag atgtgtactt ctgccccgaa
    13141 cgccacggca cctacttcgg ctcgttctac ctcaaagaca taaaagccat gagcgacccg
    13201 acctatgcca tccccactaa tttgacaggt gacctcggcg agaccatgct gcaaaaactg
    13261 actcccgatc aagccgcgct gatcacgggc ctggttgaca accagaaatc tgccctgcaa
    13321 ggaatggtag atgcccgaga acagatctcc attgaacttc gcaagttcaa agacggcagt
    13381 gcggctgatc aggcaaccat catgagcctg atggaacaat acggcgcgta cgacggtgag
    13441 atcatctaca acatggccgt gaacttcgcc gcagtcaaga gctcgctcac ctccgaacaa
    13501 caggcagaac tcgacgccct gcgcacagaa ctgctcggtg accaatctca tcccacgggc
    13561 gcctacctgt actcacaagc aatccccatg cctgaaattc caaattctga tttcctcttc
    13621 aagtaatcaa aaactgaaac gaaccatttc aaaagaaagg atgaaacaat gtacaaaaaa
    13681 atcttctcac tactcttgat ttgttccctg ctgacaaccg gtttcacctt tgcgtcaact
    13741 gcgccacagc ctgcctctgc acaagcagcg acagccacat tcgaagatgt ccccacaacc
    13801 tattgggcgt atcaatggat cgaagcgctc gctgcaaaca gcatcaccgg cggatgcagc
    13861 accacgccca aattattctg tcctgatacg cttgttaccc gcgcacaaat ggcagtcttt
    13921 ctgttgcgat ccaaacacgg caatgcctac actccacccg ctgccacagg gaccgtcttc
    13981 accgatgtac cggcaggcta ttgggcagcc gattggatcg agcaactctc agccgaaggc
    14041 atcacctcag gttgttccac cggcctatat tgcccggaaa ccatcgtctc acgcgatcag
    14101 atggcgatct tcctgctccg atctgaatat ggcagcagct acatcccgcc agccgcaacc
    14161 ggcaccgtct tcaccgatat ctcagccgat tattgggccg caagttggat tgagcaattg
    14221 gtcaacgaag gcatcaccgc cgggtgcagt gacacaggat actgccctga acagtcggtt
    14281 acacgcgcac agatggcggt ctttctcgtc cgcgcattca atctgccacc cacaccgccc
    14341 gcattcaacc tcgatcaaac cctttcagat ggcgctcaat tgaacaccat cgcctttgat
    14401 ggccttgcat ttatcaccgg cagccttggc gcagattcat tcttccctcc cggcaaagtc
    14461 gctgatttct ggggctttca atatctgcgc gacaatgacc ccagcgaaat gggacacaat
    14521 accgactttc tcacccgtgc ttcattgaac ctgctctacg tactcacttc tgaccagaaa
    14581 gccgcgctaa ttacactcgc caaaaaccag gttgcatcga tcaatgaata tggctacaaa
    14641 cgctttgtgc tgatgaaagc attccgccgc ctcgtagaag gagacctgcc cgctggaaca
    14701 accggcttaa atccagatgc agtcaaagcc tattctgcag aactctacca actcgacggc
    14761 cagattagtt acgaacgcgc acagatcatg ggcggaatca tctctgcgtt cacaaccgag
    14821 caacgcgctt atcttgatgc gatggtcgga cagggcatga cctcatggcc tgtggtgaac
    14881 gagccttcag aactcaaaga tcttacgcag gatgaaaaga tcgcagtaat gacctacgct
    14941 ggtgacatgt tcagttggta cgccggttct tttcaagccg atgtctactt ctgtcccgaa
    15001 cggcagggca cctacttcgg ttccttctat atgaaagatg cgcctgcaat gggcaatccc
    15061 gatttcacca ttccatccaa cctcaccgct gaaatgggtg acgcctttct caagactctc
    15121 acagcagatc aagcggcact gatcaacaac cttgtgactg atcagaaacc ggctttgtat
    15181 ggaatcgtca gcagcaggga ggaggtctct acaatgctgc gacaattcat ggcaggcggc
    15241 actcctgatc aaaccgcaat cctcaggctg atggaaacct atggcgagct cgatggcgag
    15301 attatcacca gctacatctt cgccttcaat cagattaacc aaaccctcac cgccagccag
    15361 cgcgcgcaat tgttgaaact gcgcgtcgat tggctgcaag atctgaacca gcccacagga
    15421 gccttcctgt actctcaagc catcaccatg cctgaaattc cgaatactga tttcctgttc
    15481 aagtaattcg taactataaa gggtcactaa ggtttttcct tagtgaccct ttgtctcttt
    15541 tgtgatcaaa aggcttatga aaaaacttct tttcactctt ctctttctgt cgctcctcgc
    15601 cgcctgtata cccgccacag aatcaccacc acagacacaa actacttctg cagcagcaac
    15661 cgattcatcc ttcatgcaaa gcgttcagcc ttcatccctg cctttgccca ccgaaacaaa
    15721 taagcccaaa gggcataacc aacctgtcg
//
LOCUS       DAOQJC010000095        15586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1793_length_15586_cov_8.612710, whole genome shotgun sequence.
ACCESSION   DAOQJC010000095 DAOQJC010000000
VERSION     DAOQJC010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15586
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1793_length_15586_cov_8.612710"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..2555)
                     /locus_tag="PLE39_13705"
     CDS             complement(165..2555)
                     /locus_tag="PLE39_13705"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE69435.1"
                     /translation="MSSRWKKVWADFWGNRGRTFLTIMTIMVGTFAVGFNSNMGAYMM
                     QSMDDDYLSANPAEAQVYAGPLDDDMVKIARTVPGVEQVQGFSSVGARIVRPDGTYTN
                     VNFIALENPGKLTLNLLKPVTGEISIPTYGKNETIFDGSAMGMGYKAGDTILVELADG
                     KRRELKLTGYMHDVTGIPFNLANTVGAYVTPDTLEWLGGDTSSYEMLAISVLEKQTDQ
                     EHVTTVAQAVADRVERAGATVFFVNVYEPGHHFAWSITQGVFFVLSILGYLTVLLSGF
                     LIINTITALMTQQTRQIGIMKAVGGGTIQITGMYVVLILGFGLGALAIAIPLANLAAE
                     EIGGGMAQWLNFYASPYKGYTSTLIQQVIVALVVPLLAAILPIYNSVRVTVREAISDY
                     GLGGNARPKADSVNQKSVLIPRPIRISIRNTFRRKVRLGITLFTLVLAGAVFIAVYNL
                     WASFDKVMQDIQGYFLADINIAFDRGYRFDKVASITENIPGVEGAEGWLEYPGTLKMG
                     DDESGTQILFVAPPSTSTLISPIISTGRWLQPGDENAVVIGNHLLKVFPDLKIGDWLT
                     IETNGKETDWQIVGTYSLPGNVSPPLLYANYEYLSQLVGEIDMAYSLRITTTQHDSVT
                     QKRINDMVQAAFEAQGVQIRSTQLSADFIRDQKAQTDILVYFMLVMATLIAIVGGLGL
                     MGTMSINVLERTREIGVMRAIGASNGDIQAIVIVEGMVIGIVSWLISIVIAIPITNIL
                     CYGVGMAILTAPMPAVYGVSGIAAWLIFTIVLGSLASALPARRASRLTVRDTLAYE"
     gene            complement(2552..3685)
                     /locus_tag="PLE39_13710"
     CDS             complement(2552..3685)
                     /locus_tag="PLE39_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE69436.1"
                     /translation="MAFFQKNNHTSTDTRPMIDLRDVNKFYKTAIGDYQALNNIDLQI
                     NAGEFVSIIGKSGSGKTTLLNMITGIDIPTNGEVWVNDTAVHKLSENKMARWRGKNLG
                     VVFQFFQLLPMISVIENIMLPMDFCRTYPMRERHQRAMQLLELVELADHAHKLPTALS
                     GGQQQRVAIARALANDPPVIIADEPTGNLDSKTAESVFSLFNDLVSKGKTIIIVTHDS
                     ALAKRTHRTALIADGEIVNEYVAKAMPTLTAAQLLQLTRSAKTLQFEPGAMILSEGTH
                     ADTFYVVSKGTVEVVLPRTNQSDVVALQLGPGKCFGEIEFFHEKKHLASVRASESGPV
                     EVVSIEYGQLKELLDQSQVTRDALHQMADKHEDEHMSIRGGSK"
     gene            complement(3799..4854)
                     /locus_tag="PLE39_13715"
     CDS             complement(3799..4854)
                     /locus_tag="PLE39_13715"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNE69437.1"
                     /translation="MKHTTLIALLVISLFATACGAAPATAEPTPIPTVLADNTIIAEG
                     NLEPIHYTELALNASGLVSEVLFSEGDQVAAGDVIARLKSNEAQTLETAQAKALAEMT
                     AAYQEVRDAQFKLDNFDVPSDFDGMTPSEAVSEMLVKLDKARADFEPYKNLSERSLAQ
                     TEAEKNSGVVTGTAKIYKQALDDAWARYRKAILWLELESTLQNANSRLTLAQKDYEAL
                     QDPSFSADTAGARAALASAEVRAPFPGLITDLNLKVGEFAASGQPVITIADNSKWVVK
                     TTDLTEIDVVKIKEGQSVTVTLDALPEVELSGTVLYISQDFSENQGDVVYEVTILLTE
                     QDPAMRWGMTAIVKFVE"
     gene            complement(5056..5406)
                     /locus_tag="PLE39_13720"
     CDS             complement(5056..5406)
                     /locus_tag="PLE39_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69438.1"
                     /translation="MPTLQTVYIMAFTPEKMDNNTLISVCRMAADMLSAKDSTFAQRM
                     GMIAKTTRMTFSNQLYVSTMHTPQTMQTTLGGWVKNENMPFAPVLGDTFFPHGMRDPK
                     GRENFFLFYFHITN"
     gene            5485..6336
                     /locus_tag="PLE39_13725"
     CDS             5485..6336
                     /locus_tag="PLE39_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69439.1"
                     /translation="MSQINLKKLNTHRTRTFNLPPNKGLSSPAQALTFIKKRGFVYFW
                     PIKGIDLPSLWAAVAGDRPVAEKHDDPGHITWGWKDNALDKKVWYYAKILRGKATMIS
                     LETAPYFYALSENYGSPDEDYLIAYQEGRLTQASKQVYETLLENGALNTLDLRKMAKL
                     SNAKDSEFNKALEQLQRDFKILPVGVAQAGAWKYSHIYEIVPRHFPDLPEQARQITES
                     QARMKLLELYFNLVGAAQMRDVVKLFGWSTEITNRIVSRMTDQGLLAKSTHPKHVGEW
                     LVLPSLV"
     gene            complement(6378..6965)
                     /locus_tag="PLE39_13730"
     CDS             complement(6378..6965)
                     /locus_tag="PLE39_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNE69440.1"
                     /translation="MSKQPSSYLSSKLEGRPKADGSGNGIYALEPINKGELVAVFGGV
                     VYEWDAFIHLPDRERSLCIQVEDRHFLVPRPIGEGDYVNHSCNPNAGLSGQIGLVAMR
                     DIKIGEEVCFDYAMSDTMPYDEFECGCGAATCRGKVGGNDWQKPELQKRYAGFFAPHV
                     QRKIDAQRKEKRAFEQAIRMAKSPKFPSVTTPVYE"
     gene            complement(7268..9247)
                     /locus_tag="PLE39_13735"
     CDS             complement(7268..9247)
                     /locus_tag="PLE39_13735"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE69441.1"
                     /translation="MPFLSPGQTLLNQYQIETFVTLTPLGELYRATDTRSNKPLALTL
                     LPKTISENIEKLKHLEAEAARLRSISHPNIVPFLGLYQTPTTNFLLEEWVNGPSLVEI
                     LARPPLSATEALVYTKAICAALEALHKQNYLHLNLIPEFIHINKRGEILVSGISAAQQ
                     INAIIEYPHGKYLRLYASPEQFTDQKLTPASDIYSLAAILYTLITGAWINGKSAPKTN
                     AEIKKAHLDLTPPAPITLNKEIPDHFSRMILWALRKNPENRLKTTTELLSSLALATQS
                     SVDQVPLRTDPKTAPVTSAVLEGWNFLPPPKPNIITEDSLPLEERMTAIAQPKKRRSG
                     MGIMTAFVVVLLLGFASLFWLVRPAPVVIPTPIQFTPFAADFTPPPTFTPEPRPTDPH
                     GGRIAFTCTRGDYNQVCIVNRDGSGYTQLSDMDASSYYPAFTPDGSSLLFASNRNGAF
                     DLYLLQFGQKQLTQLTQNIGNVVSPDYSADGQKIIFVNQVGDSLPAIWMVNADGLNPH
                     LVYAGVNEIVAVTWSPDMSQIAYAMSTGAPLEYEIYVMNSDGREHRQVSRGLQGIGGS
                     LDWSPDGKSLLIYAGPFGDKDIFSIDIASGNYTQLTDGGNNAGASYSPDGLYIVFNSL
                     RNDEQADLYIMRVDGSNQIQLTNHPEPDWGPRWVE"
     gene            9329..10348
                     /locus_tag="PLE39_13740"
     CDS             9329..10348
                     /locus_tag="PLE39_13740"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNE69442.1"
                     /translation="MNTTSSSMTLRWVDLIRVIGAFLVVMAHVAYTGAGPVLASTYYF
                     VISRVAVPLFFMVSGFLLLKKEEPYGVFFRKRAVKVAVPFLVWSVIYLLWKREGFETG
                     FSLDLVVSYVVKIVRGPRENHLWFFYALIGLYLFTPILRVFVARASKTDLFYFCGLWF
                     LVVPIFSLLQEFTPIKIGFELYFVAGYTGYFLLGHVLGLFEFSRKQLYGLAVLFLFLS
                     VLTAYLDLQSNLLKWNTQYFVSYLSVNIVLLTVAAFILLREIPIDDRWYAFLAPLGRA
                     SFGIYLVHVIVLTELEKIPAIHYWFSAGSSVYMIPLLGLFGFAVSYLIVAVVQKIPVL
                     RWAMP"
     gene            complement(10353..10553)
                     /locus_tag="PLE39_13745"
     CDS             complement(10353..10553)
                     /locus_tag="PLE39_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69443.1"
                     /translation="MSKFLDRMSEFLAHRKGLLPIIGLVLILVNLLVQFIFPGGFLAS
                     TNLFLHIGLMVAIFGLMLAWAL"
     gene            complement(10553..11917)
                     /gene="hflX"
                     /locus_tag="PLE39_13750"
     CDS             complement(10553..11917)
                     /gene="hflX"
                     /locus_tag="PLE39_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNE69444.1"
                     /translation="MAKKLPQPTTPPRERAFLVGVEIRGDKSMLPLNDSLAELALLAD
                     TAGVDVVGELTQRLDRPHVVTYIGPGKVEELKALAEETLTDVIIFDDELSPRHQRELE
                     KALSIRVLDRTALILDIFAQHAHTSEGMLQVELAQYEYNLPRLTRAWTHLERQAGGGG
                     GRAGSTGGVGLRGPGETQLEVDKRAIRKRITHIKRELEKVEAHRMRYRAQRKRSRIPT
                     VALVGYTNAGKSTLLNRIAKSEVYVADQLFATLDPTTRRVQLPGDDQALMTDTVGFIQ
                     KLPTSLIAAFHATLEEIAEADLLLHVVDISHPNALNQYQAVQETLNEIDAGHIPMITA
                     LNKADLLNHPENAIEVLKAFPKAVSISAREGTGIKDLLHLIREELYESYSPINVRLPY
                     QQGALISLFHELGQVERVEHERGGVVMQGRIPGRLLAQYASWHIKPNQQEMTENEADD
                     LEEE"
     gene            complement(11877..13157)
                     /locus_tag="PLE39_13755"
     CDS             complement(11877..13157)
                     /locus_tag="PLE39_13755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE69445.1"
                     /translation="MTVQESLQKIRTPWVERISRELASGEGVRVGFIEQLERFYELLE
                     HTVVTGNLAWLDPVLYDWGQSPTETNLEQGDYYVSFIVNRMTTLTIEVARENLGKEDA
                     LELVAAVVPIFTHSLSVVVRYEMETRVAHISSELNKTQEKLQQLDHNKSNFISVAAHE
                     LKTPLTLIEGYTTMMVDMVEQSGQSPIDSLLKGVNTGIQRLRQIIDDMIDVSLIDNNL
                     LALNFQPLWVSHMLNLLKNELKKTVTDRKQTLIVNQFEGSDLMIYGDSERLYQALYNV
                     ASNAIKYTPDHGTITIEGRLLPGFIEITIKDTGIGISPENQSRIFEKFGQLGRTDLHS
                     SGKTKFKGGGPGLGLPITRGIIEAHGGTIWVESEGYDEEKLKGSTFHILLPTRTEATD
                     PLITKFFGAPGQLKSESEKETIGKETPPTNNPTS"
     gene            complement(13236..>14138)
                     /locus_tag="PLE39_13760"
     CDS             complement(13236..>14138)
                     /locus_tag="PLE39_13760"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNE69446.1"
                     /translation="MTKRAAIIYLSSIFALLALVAGWYIVSFINREPDPVSFDSSRAY
                     ADVQTQVDFGPRIPGTDGHAKIRAWMQAELESAGWTVEIHETERMGHPVYNLIAKRGE
                     ADPQIILGAHYDTRMIADHDPQPTNLNKPVPGANDGASGVAVLLELARTLPEDSVPVW
                     LVFFDTEDNGRIEGWDWILGSRAFAEEIPVRPEAVVIVDMIGDADLNIYLEQNSDIAI
                     RTEIWGIAAQLGYGDKFINEEKYSMLDDHTPFLEAGIPAVDIIDFDYPYWHTTQDTPD
                     KVSAESLGAVGNTLLTWISGKTPK"
     assembly_gap    14139..14238
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14239..15551)
                     /gene="trmFO"
                     /locus_tag="PLE39_13765"
     CDS             complement(<14239..15551)
                     /gene="trmFO"
                     /locus_tag="PLE39_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HNE69447.1"
                     /translation="MTSLTIIGGGLAGSEAAWQVAQRGFKVRLYEMRPINPTGAHLTG
                     DLAELVCSNSLGSNLPDRASGVLKNELRLLNSMLLECAEASALPAGAALAVDREAFAR
                     LVTEKIQSHPNIEVIRAEMKEIPDSPVIIASGPLTSESLSRSIAKLSGDEHLFFFDAI
                     APIVLAESINMEIAFRASRYGQGDQDEGDYINCPFTKDEYYAFVNALQTAERIQLHSF
                     EDAIRTGVKAGQFFEGCLPIEIIAERGTDSLAYGPMRPVGLRDPRTGKRPYAVVQLRQ
                     DNLAGSLYNLVGFQTNLKFPEQRRVLRMIPGLEQAEFERYGQMHRNTFIASPKLLRPT
                     LQHITRDDLFFAGQITGVEGYMGNIATGLLAGLNAAQLLQGKPPVTLPPETMLGALCH
                     YITHADLKDFQPMKANFGILPPLELEKRIGKRERGQRYADRAANAL"
BASE COUNT         3405 a         3612 c         4405 g         4064 t
ORIGIN      
        1 gtggaaagcg cgccacaaaa tggaaccact tttaccgttt cattcaaaga tgctcatgcc
       61 tctcaggcgt aagcagagtt gaaccccgtc tgggcttgtt gtaaaaaaga gaaacgcctc
      121 gcatggaatt catccatgcg aggcgtttgg ttttcaaaag cagtttattc gtatgccaga
      181 gtatcgcgaa cggtcagccg tgatgcgcgg cgggcgggga gggcgctggc gagtgatcca
      241 agcacgatcg tgaagattag ccaggcggcg attccactca cgccgtagac agcaggcatc
      301 ggggcggtaa ggatcgccat tcccactcca tagcaaagga tgttcgtgat cgggatggcg
      361 atcacaatgc tgatcagcca actgacgatc ccgatcacca tgccttcaac aattacgatg
      421 gcttgaatgt cgccattgct ggcgccgatg gcccgcatta cgccgatttc gcgggtgcgt
      481 tccaatacgt tgatgctcat ggttcccatc aaacccagcc cgccgacgat tgcgatcaag
      541 gttgccatga ccaacatgaa atagacgagg atatcggttt gtgccttttg gtcgcggata
      601 aaatctgcgc taagctgtgt ggatctgatc tgaacacctt gcgcttcaaa ggcggcttgc
      661 accatgtcgt tgatccgctt ttgggtgacg gagtcgtgtt gggtggtggt gatgcgcagt
      721 gagtaggcca tgtcaatttc gccaacgagc tggctcaggt attcgtaatt ggcgtacaac
      781 aacggcgggc tgacattgcc aggcaggcta tacgtgccga cgatctgcca atcggtttcc
      841 ttgccattgg tttctatggt cagccaatcg ccgatcttca ggtctgggaa gaccttgagc
      901 aggtgattgc caatgacaac ggcattttca tcgccaggct gcagccagcg ccctgtggaa
      961 atgatcgggc tgataagagt ggatgtggaa ggtggggcca caaaaaggat ttgcgttccc
     1021 gattcgtcat cgcccatttt gagggtgcct gggtattcca gccagccttc tgcgccttcc
     1081 actcctggga tgttttccgt gatgctggcc actttatcaa aacggtagcc gcggtcgaag
     1141 gcaatattga tgtccgctag gaagtaaccc tgaatatcct gcatcacctt atcaaaagag
     1201 gcccacaggt tgtacaccgc aatgaatacg gctcctgcca acacaagggt gaataacgtg
     1261 attcccagac gtaccttgcg cctgaacgta ttgcggatcg aaatgcgaat tgggcgtgga
     1321 atgagcacag acttttggtt gacagagtcc gccttggggc gggcgtttcc accgaggcca
     1381 tagtcactga tcgcttcacg gaccgtcaca cgtacactgt tgtaaattgg gaggatggct
     1441 gccagcaagg gcaccaccag tgccacgatc acttgttgaa tcagtgtgga tgtataccct
     1501 ttgtatggcg aggcataaaa attcagccat tgtgccatgc cgccgccgat ttcctctgcg
     1561 gcaaggttgg ccaatggaat ggcgattgcc agcgcaccca acccaaaccc taaaatcagc
     1621 acaacataca tgcctgtgat ctgaatggtt ccaccgccta cagccttcat aataccgatc
     1681 tggcgggttt gctgggtcat caacgctgta atggtgttga taatgaggaa gccgctcagt
     1741 aaaacggtca ggtagccgag gatgctcaat acgaagaata caccctgggt gatgctccat
     1801 gcaaagtggt gcccaggctc atacacattc acgaaaaaca cggttgcgcc tgcacgctcc
     1861 acacggtctg caacggcttg cgccactgtg gttacatgct cctgatcggt ttgcttttca
     1921 aggacgctga tggcgagcat ttcatagctt gaggtgtcgc cgcccagcca ttccagcgtg
     1981 tcgggtgtta cgtatgcgcc gactgtgttg gcaaggttga atggaatgcc agtgacatca
     2041 tgcatgtacc ctgtcaactt caattcacgt ctcttgccgt cggctagttc caccaaaatg
     2101 gtgtcgccag ctttgtaacc cattcccatt gcactgccat cgaagatggt ctcgttcttt
     2161 ccgtatgtgg gaatgctgat ctcgcccgtg actggtttaa ggaggttgag cgtgagtttc
     2221 ccagggtttt ccagtgcgat gaaattgacg ttggtgtagg ttccatcagg gcggacaatg
     2281 cgcgcaccga ccgatgaaaa tccctgcacc tgctccacac cagggacggt tcgggcgatc
     2341 ttcaccatgt cgtcgtcaag cgggcctgca taaacctgcg cttcagcagg gtttgcagaa
     2401 agataatcat cgtccatgct ttgcatcata taagcaccca tgttgctgtt gaaaccaact
     2461 gcaaaggtgc ccaccatgat ggtcatgatg gtgaggaagg tccgtccgcg gttgccccaa
     2521 aaatcagccc agaccttttt ccaacgcgaa ctcatttgga gcctcctcga atggacatat
     2581 gctcgtcttc gtgcttgtct gccatctggt gcagcgcatc gcgcgtgacc tgagactggt
     2641 ccagcaattc tttgagttga ccatactcga tggagaccac ttcaacgggt ccgctttcag
     2701 aggcacggac agaagccaga tgtttctttt cgtggaagaa ttcaatttcg ccgaagcatt
     2761 tcccaggtcc aagttgaagc gccaccacat ccgattgatt ggtgcgcggc aggacaactt
     2821 ccactgtccc tttcgagacg acgtagaaag tatcggcatg agtcccttct gagaggatca
     2881 tggcgcctgg ctcgaattgc agggttttgg cactgcgtgt gagttgcagt aattgcgccg
     2941 cggtcagtgt gggcatggct ttggctacat actcgttgac aatttcaccg tcagcgatga
     3001 gggctgtgcg gtgggtacgt tttgcgaggg cgctatcgtg tgtaacgatg atgatggttt
     3061 tgcctttgga gacgaggtcg ttgaacagtg agaaaacaga ttcggctgtc tttgagtcga
     3121 ggttgcctgt gggttcatca gcgatgatga cgggcgggtc gttggcgagg gcacgggcaa
     3181 tcgccacgcg ttgctgctgt ccacccgaaa gggcggtggg gagtttgtgc gcatggtctg
     3241 cgagttccac cagttcaagc aattgcatag cccgctgatg acgttcgcgc atgggatagg
     3301 tgcggcagaa atccatcggc agcatgatat tttcgatgac tgagatcatc ggcagcagct
     3361 ggaagaactg aaatacaacg ccgaggttct tgccgcgcca gcgggccatt ttgttctcgc
     3421 tgagtttgtg taccgccgtg tcatttaccc agacttcgcc attggtaggg atgtcaatgc
     3481 ctgtgatcat gtttaacagg gtggttttgc cgctgcctga tttaccgata atgctgacaa
     3541 actcacccgc gttgatctgc aggtctatgt tattgagggc ctgataatcg ccgatggctg
     3601 ttttataaaa tttgttcaca tcgcgcaggt cgatcatggg gcgggtgtct gtgctggtgt
     3661 ggttgttttt ttgaaagaat gccattatat acgtgccttt cttgcgccga tgtttgaaga
     3721 ggggagtgca tcacatcaaa ggatgtcact cctttttgat tcagaagcag tttcaaggtc
     3781 cagcgcgaag cggttttttt attccacgaa cttgacgatg gcggtcatgc cccaacgcat
     3841 ggcagggtct tgttcggtaa gcaggatggt gacttcgtaa acaacgtcgc cctgattttc
     3901 ggaaaaatcc tggctgatgt agagaacggt gccgctcagt tccacttcgg ggagcgcatc
     3961 caacgtcact gtgaccgatt ggccttcctt gatcttgacc acgtcaattt cagtcaggtc
     4021 ggtggttttg acgacccatt ttgaattgtc ggcaatggtg atgacaggct gccccgaagc
     4081 ggcgaattct ccgactttga gattcaagtc tgtgatcagg ccagggaagg gggcgcgtac
     4141 ttcggcgctg gcgagggcgg cgcgggctcc agccgtatcc gctgaaaaag atgggtcttg
     4201 cagggcttcg taatcttttt gggcaagggt caggcgggag tttgcgtttt gcagggtcga
     4261 ttcgagttca agccagagaa tggccttgcg gtaacgtgcc caggcgtcat caagcgcttg
     4321 cttgtagatt ttcgctgtac cagtgaccac accgctgttc ttttcggctt cggtttgggc
     4381 caggctgcgt tcgctgaggt ttttgtacgg ttcgaaatca gcgcgggctt tatcaagttt
     4441 gaccagcatt tcgctgaccg cttcagatgg ggtcatccca tcgaaatcgg aggggacgtc
     4501 aaaattatcc agcttgaact gggcatcgcg cacttcctgg taggcggcgg tcatttcggc
     4561 cagtgccttg gcttgtgcgg tttcgagggt ttgggcctcg ttcgatttca ggcgggcaat
     4621 tacatcgcca gcggcgacct gatcgccttc gctgaacaag acctcgctaa ccaatccgct
     4681 ggcgttcagg gccaattcgg tgtagtggat cggttcaagg ttgccctcgg caatgatggt
     4741 gttatcggca aggacggttg gaatgggggt aggttccgct gtggcggggg cagctccaca
     4801 ggctgttgca aaaagggaga taaccaaaag agcgatcaga gttgtgtgtt tcatggtgct
     4861 ttcctttgta aatttactta tcccgaatgg gatggatttt caagggtatt tacgcgccgt
     4921 ggcagaaatg gttgcaaaaa tactccaaaa aagctaatga aaagacagcc tatcacaact
     4981 cttgaaagat tggatggctg cctctaaaaa acagttctca gggattgttt caagccctgc
     5041 tacgggctgg gtttgtcaat tggtgatgtg gaagtaaaag agaaagaagt tctccctgcc
     5101 tttcgggtct ctcataccat gcgggaagaa ggtgtcaccg agcacgggcg caaagggcat
     5161 attctcgttt tttacccagc cgcccagtgt ggtttgcatg gtttgcgggg tgtgcatggt
     5221 ggatacataa agttgatttg agaaggtcat gcgcgtggtt ttggcgatca ttcccatccg
     5281 ctgggcgaat gtactgtctt ttgctgagag catatccgct gccatgcggc acacgctgat
     5341 caaggtattg ttgtccattt tttcgggggt aaaagccatg atgtaaacag tttgaagtgt
     5401 gggcatggaa ttctcctttt tggaaattat agtgtattcg ctccagccgc atcaggtttc
     5461 aaacgtatat tacaattgac ccatatgtcc cagatcaatt tgaagaaatt aaacacgcat
     5521 cgcacgcgga ccttcaacct gcctcccaac aaggggcttt catcgccagc ccaggcattg
     5581 accttcatca aaaaacgcgg cttcgtttac ttctggccca tcaaaggcat tgacctgccc
     5641 tccctgtggg cagcggttgc gggcgaccgt cccgttgctg aaaagcacga tgacccaggt
     5701 cacatcacct ggggttggaa agataacgcc cttgataaga aggtttggta ttacgccaag
     5761 atattacgcg gcaaagccac aatgatctca cttgagaccg ccccttattt ttacgccctc
     5821 tctgaaaatt atggttcgcc tgatgaagat tatctcattg cctatcaaga ggggcggctg
     5881 acacaggctt caaaacaagt ttatgaaacc ctgcttgaaa acggcgccct gaacaccctt
     5941 gatctgcgca aaatggcgaa gctatccaat gccaaagatt ctgaattcaa caaagccctg
     6001 gagcaactgc aaagagattt caagatcctg ccagtgggtg tggcgcaggc cggcgcatgg
     6061 aaatattccc acatctacga gatcgttcca cggcacttcc ctgacctgcc cgaacaggca
     6121 cgccagatca cagaatcgca agcgcggatg aaactgctgg aactctattt caaccttgtc
     6181 ggcgcggcac agatgaggga tgttgtgaaa ttgtttggct ggtccaccga gataacgaat
     6241 cgtatcgtga gtagaatgac tgatcagggc ttgcttgcaa aatcaaccca tccaaaacat
     6301 gtcggggaat ggcttgtgct cccgtccctt gtataatgga taaaaaactc ccgtgaattt
     6361 cacgggagtt ttttgcttta ttcgtaaacc ggagttgtca cagaaggaaa cttcggtgat
     6421 tttgccatgc ggatggcctg ttcaaaagcc cgcttctctt tgcgctgagc atcgatcttg
     6481 cgctgaacgt gcggcgcaaa gaaccctgca tagcgttttt gtaactcagg cttttgccag
     6541 tcattgccgc caacttttcc gcggcatgtg gcggctccgc acccacattc gaactcatcg
     6601 tagggcatgg tgtcgctcat ggcataatca aaacaaactt cctcgccgat tttgatgtct
     6661 cgcattgcaa cgagtccaat ctggcccgaa agacctgcat tcggattgca cgagtgattg
     6721 acataatctc cttctccaat ggggcgcggc accaggaaat gacgatcttc aacctggatg
     6781 caaaggctgc gctcacggtc aggaaggtgg ataaaggcgt cccattcata aacgacgccc
     6841 ccgaaaacgg ctacgagctc acctttgttg atgggctcca gggcataaat gccgtttcca
     6901 ctgccatcgg ccttcgggcg gccttccaac ttagaagaca gataggaact tggttgcttg
     6961 gacattcata aaaactcctt ttttggatta aagattaaca gcgccctccc cggtggtcga
     7021 gccgggaaaa acgccattga tacagcaatc atacaataaa ttttctgcat gtaaaggttt
     7081 ttgtaatacc aaatcccttg catttcgtga tgatttttta tttcaaaccc tcttgcacgt
     7141 tatttaaatt tccccgctaa tgatgtgcat gcgtaataaa tatttacccg aattggatga
     7201 attaccgctt cacgcttcgc gccgctgaaa actgattctg ttcaaaatac atactcttcc
     7261 tgtgaattta ttccacccaa cgcggccccc aatcaggctc agggtgattg gtgagttgaa
     7321 tctgattgct tccgtccaca cgcatgatgt agagatcagc ctgctcgtcg ttgcgcagtg
     7381 agttgaagac gatgtacaac ccgtctggag aataggatgc gcctgcattg ttgccgccat
     7441 cggtgagttg ggtgtaattt ccactggcga tgtcgattga gaagatgtct ttgtcaccga
     7501 atgggcctgc ataaataagc agactcttcc catcaggaga ccaatcgagg ctgccgccaa
     7561 tgccttgcaa tccgcgcgaa acctggcgat gttctcttcc atccgaattc atgacgtaga
     7621 tctcatattc caatggcgcg cccgtagaca tggcgtatgc gatctgactc atatcaggtg
     7681 accacgttac tgccacgatt tcgttgaccc ccgcataaac gaggtgcgga ttcaatccat
     7741 cagcattgac catccagatc gcgggcaggc tgtcgcccac ctggttgacg aagatgattt
     7801 tctgcccgtc ggcagagtaa tcgggtgaga ccacattgcc aatattttgg gttaattgcg
     7861 tcagttgctt ttgcccgaac tgtaaaagat acaaatcaaa cgccccgttg cgattggagg
     7921 cgaacaacag cgagcttcca tcaggagtga aggcggggta gtaactgctg gcatccatat
     7981 ctgaaagttg agtgtagcca gagccgtcac ggttgacaat gcatacctgg ttgtagtcac
     8041 ctcgtgtaca ggtgaaggca atgcgacctc cgtgtgggtc agtggggcgt ggctcggggg
     8101 tgaatgtggg cggcggagta aaatctgcgg caaagggcgt gaactggatc ggggttggaa
     8161 tcacgacggg agcgggacgt accagccaaa acaatgaagc gaatcccaaa agcagcacaa
     8221 caacgaatgc ggtcatgatg cccatgcctg atctgcgctt tttaggctgg gcaattgcag
     8281 tcatgcgctc ttcgagcggc agtgaatctt ctgtgatgat gttaggcttg ggcggcggca
     8341 ggaaattcca tccctcaaga actgccgagg tgacaggagc agttttcggg tcggtgcgaa
     8401 gcggcacttg atccactgaa gattgggttg ccaacgcaag cgatgagagt aattcggtgg
     8461 tggtcttgag tcgattttcg ggattttttc gcagggccca taggatcatc cgcgagaaat
     8521 gatcgggaat ctccttgttg agggtgatgg gtgctggcgg cgtgagatca agatgagctt
     8581 ttttaatttc agcattggtt tttggcgcgg atttaccgtt gatccatgcg cctgtgatga
     8641 gtgtgtaaag tatggcggcc agggagtaaa tatctgatgc tggggtgagc ttttgatcgg
     8701 tgaactgttc tggtgaagcg tacagacgca gatatttccc atgcggatat tcgatgatgg
     8761 cgttgatctg ttgggcggcg ctgattccgc tgaccaaaat ttcaccgcgt ttgttgatgt
     8821 gaatgaattc aggaatgagg tttaagtgca ggtagttctg tttgtgcagg gcttcgagtg
     8881 cggcacagat tgcctttgtg tacaccaagg cttcagttgc ggagagggga ggccttgcaa
     8941 gtatttcgac aagcgacggg ccatttaccc attcttccag aaggaagttt gtggttgggg
     9001 tttggtataa gccaagaaaa gggacaatgt ttggatgtga gatgctgcgc agtctggcgg
     9061 cttctgcttc aaggtgtttc agcttttcga tattttccga aatcgttttt ggcagaaggg
     9121 taagggccag cggtttgttg ctgcgcgtgt cggttgcgcg gtaaagctcc ccaagcggtg
     9181 tgagtgtgac gaacgtttcg atttgatatt gattgaggag agtctggcct ggcgacagaa
     9241 atggcatgtg cggattttaa ttcaaattgc cctgttgtgg acaaggtata atatttttca
     9301 gcaggcaatt cgttttttag gaatcaggat gaatactaca tcttccagta tgacgcttcg
     9361 atgggtggat ttgatccgtg taattggtgc tttccttgtg gtgatggctc atgttgctta
     9421 tacgggagca ggccctgtgc tggcttcgac ctattatttt gtgatttcgc gtgtggcagt
     9481 gcctttgttc tttatggtga gcgggttttt acttttgaaa aaagaggaac cctatggggt
     9541 gttttttaga aaacgtgcgg tgaaggtggc tgtgcctttt cttgtatggt cggtaattta
     9601 tctgctttgg aagcgtgaag gctttgaaac gggcttttcg ctggaccttg tagtgagcta
     9661 tgtggtgaag attgtccgcg gtccgcgtga gaaccatctt tggttttttt atgcgttgat
     9721 cggcctgtat ttatttacgc cgatcttgcg ggtgtttgtg gcgcgggcat ccaaaacaga
     9781 tttattttat ttctgcggac tgtggttttt ggttgtgccc attttttccc ttttgcagga
     9841 atttacgccg atcaagatcg ggtttgagtt gtattttgtt gctggatata cggggtattt
     9901 tttgctcggc catgtacttg gccttttcga attttcccgc aaacagttgt acggattggc
     9961 tgtcctgttc ctttttcttt ccgttctaac tgcttatttg gatcttcagt cgaatttgct
    10021 taagtggaat acccagtact ttgtcagtta tttgagcgtg aacattgttt tgttgactgt
    10081 tgcggcattt atcctgctgc gtgaaattcc aattgatgac cggtggtatg catttctggc
    10141 tccacttggg cgtgccagtt ttggaattta tctggtccat gtgatcgttt tgaccgagct
    10201 tgaaaaaata cccgccatcc attactggtt ttcggcgggt tcttcggtat atatgattcc
    10261 cctgttgggt ttatttggtt ttgcagtttc ctatctgatc gtggctgttg ttcagaagat
    10321 cccagtgtta cgctgggcca tgccgtaaaa aattacaacg cccaagccag catcaagccg
    10381 aaaatggcaa ccatcaatcc aatgtgcagg aaaagattgg tggaggctaa aaacccgcct
    10441 gggaaaataa attgaacgag taaattgacc agaattagaa caaggccaat aatggggagc
    10501 aatccttttc gatgcgccag aaattcagac atgcgatcta aaaacttgga cattattcct
    10561 cctccagatc atcggcttcg ttttctgtca tttcctgctg gtttggtttg atatgccatg
    10621 aggcgtattg agcaagcagc cgtccgggga ttcgtccctg catgaccacg ccgccgcgtt
    10681 catgttccac gcgttccacc tgacccaatt catgaaacaa cgaaatgagc gcgccctgtt
    10741 ggtagggaag gcgcacattg atcgggctgt aggattcgta cagttcctca cggatcaaat
    10801 gcagcaaatc tttgatgcct gtcccttcgc gcgcagaaat ggagactgct ttgggaaagg
    10861 ctttcaggac ctcgatcgcg ttctccgggt ggttgagcaa atcggctttg ttcagggctg
    10921 tgatcatcgg aatatgacct gcgtcaatct cgttcaaggt ttcctgcact gcctgatatt
    10981 ggttgagcgc attggggtga gaaatatcaa cgacatgtaa aagtagatcg gcttccgcga
    11041 tttcttccag ggtggcatgg aatgcggcaa ttaacgaagt gggaagtttt tgaatgaatc
    11101 caaccgtgtc ggtcatcaac gcttgatcgt cgccgggcag ctgtacacga cgggtggttg
    11161 ggtcaagtgt ggcaaatagc tggtctgcta cgtagacctc agatttggca atgcgattga
    11221 gcagggtgga ttttccagca ttggtgtaac caaccagcgc cacggttggg atgcgtgaac
    11281 gtttgcgctg ggcacgatac cgcatgcggt gtgcttccac cttttcgagt tcgcgtttga
    11341 tgtgggtgat gcgtttgcga atggcgcgtt tgtccacttc gagctgggtt tcaccgggac
    11401 cgcgtagacc aaccccgcct gttgaacctg cccgtccgcc gccgccgcct gcctgtcttt
    11461 cgagatgggt ccatgcacgg gtgaggcgtg gcaggttgta ttcgtattgt gccaattcca
    11521 cttgcaacat gccttcactg gtgtgggcgt gttgagcaaa gatatcgagg ataagggcgg
    11581 ttcggtcaag cacccgaatg gagagggcct tttccaattc gcgttggtga cggggagaga
    11641 gttcatcatc gaaaatgatg acatcggtga gtgtttcttc agcaagtgct tttaattctt
    11701 ccactttgcc ggggccgatg taggtgacaa cgtgagggcg gtccagcctt tgggtgagtt
    11761 caccgaccac gtctacgcct gcagtgtctg cgagcagagc aagttcggca agtgagtcgt
    11821 ttaacgggag catggattta tccccgcgaa tctcaacgcc gactaaaaag gcgcgttcac
    11881 gaggtggggt tgttggttgg gggagtttct ttgccaatgg tttctttttc cgattctgat
    11941 tttaactgcc cgggtgcgcc gaaaaacttg gtaattagcg gatccgtcgc ttctgtgcgt
    12001 gtaggcaata ggatgtgaaa ggttgagcct ttcaattttt cttcatcata cccttcggat
    12061 tctacccaga tggttccgcc atgcgcttcg ataattccgc gggtgatggg gaggccgagg
    12121 ccggggccgc ctcccttgaa tttggtcttg ccgctcgaat gcaagtcggt gcgcccaagc
    12181 tgaccgaact tttcgaagat acgggattga ttttcaggtg aaatgccaat gcccgtgtct
    12241 ttgatcgtga tctcaatgaa gccaggcagc agcctgcctt ctatggtgat cgtgccgtgg
    12301 tcgggagtat atttgattgc gttggaagct acattgtaaa gcgcctgata gagccgttcc
    12361 gaatcgccgt agatcatcaa gtcactgcct tcgaattggt tgacgatcaa cgtttgtttt
    12421 cggtcggtaa cggtcttttt taattcgttt ttcaacaagt tcagcatgtg gctgacccaa
    12481 agcggctgga agttgagtgc aagcagattg ttatcgatga gcgagacatc gatcatatcg
    12541 tcgatgatct ggcgcaggcg ttgaatgcct gtgttgactc ccttcaacaa actgtcaatg
    12601 ggcgattggc ctgattgctc gaccatgtcc accatcatgg tagtgtagcc ttcgatgagg
    12661 gtgagtggcg tcttgagttc gtgggcagcc accgaaataa aattggattt gttatggtca
    12721 agctgttgca gtttttcctg ggtcttgttc agttcactgg aaatatgtgc aacgcgggtt
    12781 tccatttcgt agcgaacaac cacactcagg ctgtgggtaa agatggggac aacggctgca
    12841 accaactcaa gcgcatcttc tttccctaga ttttcacgcg ccacttcaat ggtaagcgtg
    12901 gtcatacggt tgactatgaa agaaacgtag taatctccct gttccagatt cgtttcggtg
    12961 ggagattgcc cccaatcgta caacaccggg tcaagccagg ccagattgcc cgttacaacc
    13021 gtgtgttcga ggagttcgta aaaacgttca agctgttcaa taaaacccac gcgaacgccc
    13081 tcgccgcttg cgagttcacg ggagatgcgt tccacccacg gagtgcgaat tttttgcagg
    13141 gattcctgta ccgtcattgc tgtaagttta cgccttgttt agacttttga ccatataaat
    13201 atttgaaaat gcggccttgg tggtttcgtg cggcgctatt tgggagtttt tccactgatc
    13261 caggtcagca gggtattgcc aacggcgcca aggctttcag cagagacttt gtcgggagtg
    13321 tcttgcgtgg tgtgccagta gggataatca aagtcgatga tgtcaacggc ggggatgcca
    13381 gcttcgagga agggggtgtg gtcatcgagc atggagtatt tttcttcgtt gatgaattta
    13441 tcgccgtagc caagttgggc ggcaatgccc cagatctcag tgcggatggc gatgtcggaa
    13501 ttctgctcga ggtagatgtt caagtctgca tcgccgatca tatctacgat gacaactgct
    13561 tcaggccgaa cgggaatttc ttcagcgaag gcgcgtgaac ctaaaatcca atcccagcct
    13621 tctatgcggc cgttgtcttc ggtgtcgaaa aagaccagcc aaacagggac actgtcttcg
    13681 gggagggtgc gtgccagttc aagcaaaacc gccactccag aggctccatc gttggcaccg
    13741 gggacgggct tgttgaggtt ggtgggctgc gggtcgtgat cggcgatcat gcgggtatcg
    13801 tagtgtgcgc cgagaatgat ctgtgggtct gcttcgccgc gtttggcgat gagattgtaa
    13861 acagggtgtc ccatgcgttc ggtttcgtgg atttcgacgg tccaacccgc ggattcgagt
    13921 tcggcttgca tccatgcgcg gattttggcg tgaccgtctg tgccaggaat gcgcggaccg
    13981 aaatcaacct gcgtttgcac gtctgcataa gcgcgggaac tgtcgaagga aacaggatca
    14041 ggttcacggt taataaaaga gacgatgtac cagcctgcaa ccaaggccag aagagcgaaa
    14101 atcgaagata ggtagatgat ggcggcgcgt ttggtcatnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnag tgcgttggcg gcacggtcgg cgtagcgttg accgcgttcg
    14281 cgtttgccga tgcgcttttc gagttcgagc ggtgggagga tgccgaaatt ggctttcatg
    14341 ggttggaagt ctttgaggtc ggcatgtgta atgtagtgac agagggcgcc aagcatggtt
    14401 tcgggcggca gggtgacggg gggcttgcct tgaagcaatt gtgcggcgtt gaggcccgcc
    14461 agcaaccctg tggcgatgtt gcccatgtaa ccttccacgc ctgtgatctg acctgcaaag
    14521 aaaaggtcat cacgggtgat gtgttgaagg gtggggcgca aaagttttgg tgaggcgatg
    14581 aaggtgttgc ggtgcatttg gccgtagcgt tcgaactcgg cttgttccaa cccagggatc
    14641 atgcgtagaa cgcgcctttg ttcggggaat ttgagattgg tttgaaagcc aacgaggtta
    14701 tacaaactgc ctgcaaggtt atcctgccga agttggacga cggcataggg gcgtttgcct
    14761 gtgcgcggat cgcgcaaacc gacagggcgc atggggccat aggccagtga gtcggttccg
    14821 cgctcggcga tgatctcaat gggcaggcaa ccttcaaaga actgacctgc tttgacgcct
    14881 gtgcgaatag catcttcaaa cgagtggagt tgaatccgtt cggcggtttg caaggcgttg
    14941 acaaaggcgt agtattcgtc tttggtgaag gggcagttga tgtagtcgcc ttcgtcctga
    15001 tcgccttgcc cgtagcgcga ggcacggaag gcaatttcca tgttgatgga ttcggcgagg
    15061 acgatggggg caatggcatc gaagaaaaag aggtgttcgt cgccgcttaa tttggcaatg
    15121 gaccgcgaaa ggctttctga ggtgagtggt ccgctggcga tgatgacggg agaatcggga
    15181 atctctttca tctctgcgcg gatgacttcg atgttggggt ggctttggat tttttcagtg
    15241 acgaggcggg caaaggcttc ccggtctaca gcgagggctg cgcctgcagg gagggccgat
    15301 gcttcggcgc attcaagcag catggaattt aacagcctga gctcgttttt gaggacgccc
    15361 gaggcgcggt cgggtaggtt ggagcccagc gagttggagc agaccaactc ggcaaggtcg
    15421 cctgtgaggt gtgcgcctgt ggggtttatc gggcgcattt cgtacaagcg gactttgaat
    15481 ccacgctggg ctacctgcca cgcggcttca ctgccagcca gtccgccgcc gatgatggtg
    15541 agggaggtca tagggagggt attataaagg aggaaagagg aaagaa
//
LOCUS       DAOQJC010000096        15402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1826_length_15402_cov_11.123412, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000096 DAOQJC010000000
VERSION     DAOQJC010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15402
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1826_length_15402_cov_11.123412"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            522..1328
                     /locus_tag="PLE39_13770"
     CDS             522..1328
                     /locus_tag="PLE39_13770"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNE69448.1"
                     /translation="MALRGNLRDFTITQLLNLINLASKTGTLILDGPSEQAYVSFRDG
                     KLAYARIGKEDGSLALVLHKANKINVNQYRAIADRAGQMTDKELGLLLINAGYVTQED
                     ILLNLQGYFTEVVRKLFTWVEGIFRFENEMLPPDDRINVRLDLENIIIEGSRQLRELE
                     QLQDEIPSLDMALKFTERPLTNVNLSIEEWKVVKFVDPKNTMRQIASTNKLSDLDIRR
                     IVYGLLQAGLVELVRPQGAVPPPNPKQFPVQNKEEQKSLINKLIGRIRTM"
     gene            1355..1894
                     /locus_tag="PLE39_13775"
     CDS             1355..1894
                     /locus_tag="PLE39_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HNE69449.1"
                     /translation="MQTVKMVVTGPFSAGKTQFIQSVSEIDVVATERRISSDEERSVK
                     SATTVAMDFGRITVDEDLVLYLFGTPGQKRFDFMWEILSEGMLGFIVMVDSTRPETFR
                     EARSILETFRAYAPTPFVVAANKQDVDDAWDLEDVRHALRLDGDTKLLPCVATNRDTV
                     KTVLLELLYSILKEMDSEK"
     gene            1915..2712
                     /locus_tag="PLE39_13780"
     CDS             1915..2712
                     /locus_tag="PLE39_13780"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE69450.1"
                     /translation="MSSITTDQGIVHYEVYGRGRPVILLHGYQGSWGLWQQTMGYLGA
                     FYRTYALDFWGFGESGTKRETYAVKDFVGLVNQFMEQLGIIRAPLVGHSMGGTVSLLT
                     AMQYPDRVEKVVVIGSPISGSSLYFFPRVFGYRAFGWLTYHNLWIYKSFYRILAPYYS
                     KDKNWAAMMDRDVSRTTLEAFFASIGSLRRTDLRPSLHTIQVPVMGMYGDNDIVVNPN
                     QWKPLQEGVAKSHIERFPKAGHFIMLDEPLEFTQKLKAFLDQENPAS"
     gene            2739..3329
                     /locus_tag="PLE39_13785"
     CDS             2739..3329
                     /locus_tag="PLE39_13785"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HNE69451.1"
                     /translation="MGRIILQGMEEVMGVNGVETTLHLGSLDHIIQNRPTATSDRTFP
                     FEVVSAILASLEMVYGPHGGRGVALRVGRSTFRYGLKEYGSMLGLTEMAFRLLPLRTK
                     LRTGARVFAELFNKHTDQKVRVEEVENKIYWIIESCPLCWDRKADEAICHLAVGVLQE
                     ALYWLSGGKVFSVEETECIARGNATCTIVIDQIPLS"
     gene            3536..4681
                     /locus_tag="PLE39_13790"
     CDS             3536..4681
                     /locus_tag="PLE39_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69452.1"
                     /translation="MELKQGERLQPVREQAIVYRDNLFFDKYYIQAFMDEAIRLNRPV
                     RAAFRSDDPAFREHALPLSTSYTPAGSLYLADLWYYPNGPVAGAEPLVIDMQAREIGY
                     YHVPTYMGDRSGDLVFQVPLRAMMAIDSWVHVFVADMVFGQFAHGARFEKRLNEELGF
                     KLKIIGKAIYEGRQVLECSELVKVGKGCVIDPSAVIHGPAIIGDNVTINAGAVIENSI
                     IGNNVNISQDVQVMLSVVGDGAFLPFRAALFMTTIMENSMLAQNTCLQMCVVGRNTFI
                     GAGSTWTDYNLIPAPIRARDGNGKLSLSNRPVMGGCVGHNCRIGSGMIVYPARTIESD
                     VVLAASKERRVIDRDIAFDDSDHHHLKLAHLHQTPYHKQSKGDSESW"
     gene            4709..5797
                     /locus_tag="PLE39_13795"
     CDS             4709..5797
                     /locus_tag="PLE39_13795"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69453.1"
                     /translation="MDSFAFIIHPIDPKRDVSRKFPFLGRVLTERQIDFFSTFFPPVY
                     ISEIEGITSQSSGKTIKGWFLACPYTPRRMMELPERTVYRKIIQTGRMAEKLGANILG
                     LGAFTSVVGDAGVTIANKLDIPVTTGDSFTVAMAVQAIREAAQVMDIKMESATVAVVG
                     ATGAIGRVCAELLAGEAERVLLVARDEKKLEVLRDRLKVQASSELCISTKMDVLKDAD
                     LILTVTSSIHDVIRPEHLKPGSVVCDVARPRDVSAMVAAVRDDILVIDGGMVDVPGAV
                     DFHFNFGFPDGKAYACMAETIALALEGRFEDYTVGKDITLERVQDITAIAEKHGFRMS
                     GFRSFEREVTIEQIEAVRQNARKGLRRA"
     gene            5810..6715
                     /locus_tag="PLE39_13800"
     CDS             5810..6715
                     /locus_tag="PLE39_13800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE69454.1"
                     /translation="MGNARLLVVEDDVDIANMLKIYFTSLQYDVDVANRGRDALDKTK
                     QVLPHLIVLDIMLPDIDGYEVCRNLRTSTRTSHIPVIFLTQKDERSDKLQGLELGADD
                     YITKPFDIEELKLRVQGAIRRSERESLTDPRSGLPAGRLIEEQLRRIIREKDWALLDV
                     RVNNFEAFKDVYGFVAGDDVVRFAAMMIGEVVDELGTTSDFIGHAGGDNFIIITTNDA
                     APGIRQKLKDRFANEVQSHYNFIDRQQGFIQAPKAEGGVEKVGFMYLSAGLVSPSLQS
                     FADIREITELAAEARRQDTAATSAA"
     gene            6733..14172
                     /locus_tag="PLE39_13805"
     CDS             6733..14172
                     /locus_tag="PLE39_13805"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE69455.1"
                     /translation="MSSGLGLNLLIAGLIFGALVLVILRVLARTPSAVAPNIAAANSY
                     TIPEFSRSNDNDAIIILAPGGRVEYISALARTFFDLRENEPYDLERLSRRARPSDDFL
                     DLCVSSGYKRLSINGKLVEVASYEVPGTFPVMLISLRSKEFAPALEQSDGASEEILRV
                     VTEFSQSIATSLDLETTAQSILDNVGRLVPSDVLELKLWNSEDQDLDLYRSQYANLGS
                     NGSGRMMTSYFGRLTRQLVESRTPILFGDVRSRPDMNANEEVLPIQSYLGIPLIAGGE
                     LVGSLEAGQTGSGAFGQYDLNLLLLVSGQAAVAIRNARLYVNEQKRASELAGLANLNQ
                     TLGAVRDMQELFTRLVDSVAPLFQAEIIGFLLYDEEKHTMEGKSPFRGLPAHLVDIYR
                     VTVPAESLAEKVITGQQPILTLNAAGDENWRALGLSDIAMAASLRDIALMPLLSSNRM
                     LGYLQIGHHTRGASNFGVEEVRLMSIVANQAAAIIENVLLVQQARGRAQRSDALRRIA
                     SLSGSSATLEEILKFSIRELSNLFQADAGAIFLMDEARGELRLRREATFGLPKDISSS
                     FIQIFMDDPSYRSTVSGSQKPFISGHLSTDRRVLTVYRPLTTTLSLESTIVVPLIVRD
                     RSIGELMLGSFKPDYFNAYDLQVIATAAGQLATAVESSSLLNQTDDSLRRRVDQLSAV
                     TRIGRELGASLDISHLIKTIHEESLHIVQATCSSVFLFRQQGESAGAEIEFSAGCGSR
                     ELSPAETDVLMTREPRIIAEFSQNDQPLPHDGVRSALIVPITYQARLIGLISLHSNKV
                     NAYSASDIELVQILAVQAGISLSNAQRYQSEKRSAELMRRRSDTLVRLTDVSYNLGHD
                     QPLDQALQIIARGIRESTPFRVVLMSMVETETGMLRRLTAVGIPRETMNELMARKQPL
                     ASLQQLMRPEFKISRSYFIPIDLSPVMPSDVHMVTLEVSSSAEKTPNSWDPDDALLIP
                     LENAEGQIVGLISLDDPSNGLRPDQATIESVEVFAAQAALLISNTLRQGELRNRIDSL
                     SAGLLRQQKLIDITQNDLPILLHKDLEQTISLYNLDQRAQRVRAGLAITKSVSRQLDA
                     VSALNALGRETLTQLGMAIALVAENTPDGPRLMHVLGSLPRSTNVESLFGQRNPLRAC
                     LQTGNPILISSLDENDEWRDATLLTSLRAKSVICLPVLVENKTVAAMLAISHEALPDF
                     TDEDSKVYLQISQQASLILQNISLLSQTRRRLDEVNLLLDFSRQLSGMDPDAIVRSLL
                     DSAIRVLQHAHAGVALVWNQQTETLVPRAAAGYADNTTMLAINYRQGEALPGTVFMNR
                     FARRVDEVNFVRDYNLSTENLALYRQATGGRLPVSSLLVPIGTADQNLGLLVLDNFNA
                     TSAFSHEDEALLLSLTQQIALSLDNVRLVQATQERAGQLHALNNAAASLTSSLRSDQL
                     INSLLDQLVPIIQYDTATLWLRDKDRLAVVSARGFPDTEERLGLSVAVQDSALFKEMA
                     QTGQPILVNDVREDSRFMPVDAPRLSWLGIPMISKGEMVGVLAVEKWQAHYYTREQIQ
                     VSLTFASQSAVSLDNARMYEDSVSRATELDQRSQRLATLNRFASALTGLLDTDQILNV
                     TANELLKGLGAKRVSVVTFERGRAYWKISSPRTRIKLPRLLPDSPIFSRLRESLGIFN
                     TDDARNEPDLVPLEEMLGDNPTALLVLPLTSGQNLSALIFAQTSGDARFGFNELEIAK
                     TITNQATVALENARLFQLSVRTAERFAILNETSSLVNSSLNQEDVYLAARKAAERLVP
                     LDSFVITLLDIEKNEIDPVYMIDRGKRISADRVPFGQGMSSQVISTGKPVLISNSEQA
                     NDINTVLAGSSDDEEETRSIVAVPMTLGGKTLGMLSAQSYQEGMYTEEDVQILGTLAN
                     QVVVAIQNGRLFAETQSLASQFEKRVIERTAQLQREQQNTETLLRILTEVSSSLDLDR
                     ALNRTLSLLNEAIGAEQGTIMLLHAEDNLLHYRAGYGYLSDRNDTSGRGFTLRVGEGL
                     AGWVVQNREAVLIDDLHEDPRWVRSSASGMDHRSAIVAPMQVGEDVIGVLMVFQRATN
                     FFSSEMLNLVRAIGGQVAVAINNAHLYELIRDQAERLGVMLRKEQEDASRSQAILEAV
                     ADGVLVTGADNRITFVNTSTERILALDEARLLGNPLDVFGGLFGKAASTWMETIRRWS
                     EDPSSYQSGDSYAEQLELENERIALVHLAPVILQNDFLGTVSIFRDITHEVEVDRLKS
                     EFVATVSHELRTPMTAIKGYVDILTMGAAGALTENQLHFLDIVRNNIERLNILVNDLL
                     DISRIEAGRVTLNPQSVNLREIAEDVIAETLRRSQNENKPMALSLDAPAKMPQVMGDS
                     DRVRQIIGNLVDNAFNYTPSNGTIRVNIHQEKDEVQVDVQDNGVGIALQDQGRIFDRF
                     FRGEHPLVLATPGTGLGLPIVRQLVEMHSGRIWMTSTGVPGEGSTFSFTLPTVHNGV"
     gene            14173..14547
                     /locus_tag="PLE39_13810"
     CDS             14173..14547
                     /locus_tag="PLE39_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE69456.1"
                     /translation="MARILIAEDEPDIRELVAFTLRFAGHEVTATANGEEALQQAGQI
                     IPDIIIMDVRMPRMTGYDACRAMKADPILKDIPVVFLSAKGQDSEIQTGLEAGAEEYL
                     LKPFAPDQLSERVKAILSKFGK"
     gene            14555..15343
                     /locus_tag="PLE39_13815"
     CDS             14555..15343
                     /locus_tag="PLE39_13815"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE69457.1"
                     /translation="MSAIILDGSMVHYEALGRGRPIVFLHDWVGSWRYWVNAMQVAST
                     SFRAYALDLFGFGDTSRDPLRYSLDKQSELVNRFLQEMGIGKVALVGHGLGALVGFTY
                     LKQFPSTVDRMMAVNCPFEYDSVNTRLRTAAMPELVDWLAGRTPEAVSALSDAAKADP
                     QAVSASMASLQGNNYFAAIRDLGVPCLFAYGSEDPAISMPTPDRVDSLPMHMHLVTLE
                     ESSHFPMVDNPTRFNRLMTDFLALDSGASPRELQMKEEWKRRVR"
BASE COUNT         3677 a         3798 c         3971 g         3956 t
ORIGIN      
        1 gagccctgcc aatcggccag atagggggtc tctggcaatg gggcagtggc cggggcttcg
       61 gttgcttccg gggatggagc ggtggtgggg gtgccgccgc aggccgctaa aatagcgccg
      121 acaacaatca tcacaccgag cagtactaac attttcttga agttcatttt gcattctcct
      181 ttatcaaagt tttggaaaac atctaatatt tctcgtttat ttctgtgccg cctcctttag
      241 tgaaataatg ctcaatctat tcttgggcat ttcgagcaaa tacctccgaa ttctatgttt
      301 tttgaagatt aaaaacaagt taatttgcgc catcatgcac gtgactaatg tcacatatag
      361 ggcttttgta attgcctaaa attggcgagg atgtgaataa aacagttatt cagacgttgt
      421 ttccaccctc acaagaatga taagcaggtt aattgacgca atgccacctt gcatgtataa
      481 ttttcatcta gtcatccatg gatggcgaat atagaggata tatggccctg cgtggaaatt
      541 tacgtgattt tacaatcacc caattactta atttgatcaa cctcgccagc aaaaccggga
      601 ctttaatttt ggacggtcct tcggagcagg catatgtttc ctttcgtgat gggaagctag
      661 cttacgcccg tatcggcaag gaagatggca gccttgcgtt agtgttgcat aaggcgaaca
      721 agatcaatgt aaaccaatac cgtgcaattg cggatcgtgc aggccagatg accgataagg
      781 aactcggcct gcttcttatt aatgccgggt atgtaacaca ggaagatatc ctgcttaatc
      841 tgcaggggta tttcaccgaa gttgtaagaa aactcttcac atgggtggag ggaatcttcc
      901 gttttgaaaa tgaaatgctt cccccggatg accgcatcaa tgtgcgcctt gaccttgaga
      961 atattattat tgaaggatcg cgccaactcc gtgagttgga gcagttgcag gatgaaattc
     1021 cgagccttga tatggctttg aaatttacag agcgtccgct taccaatgtc aacctcagca
     1081 ttgaggaatg gaaagtggtt aagtttgtgg acccgaaaaa taccatgcgc cagatcgcaa
     1141 gcacgaacaa gttgagcgat cttgatatcc gcaggattgt gtatgggttg ctgcaggcag
     1201 gccttgtaga gttggtacgt ccacaaggtg cggttccgcc gcctaacccg aaacaattcc
     1261 cggttcagaa taaagaagaa cagaaatcgt tgatcaataa attgattggc cgcattcgaa
     1321 caatgtaatc atcagggcta gaaggataga aattatgcaa acggtcaaaa tggtagtgac
     1381 aggtccattc agtgctggca agacgcagtt cattcagtct gtcagtgaaa tagatgtggt
     1441 tgcaacggaa cgcaggattt cttctgacga agagcgttct gtcaagtctg ctactacagt
     1501 ggcgatggat ttcgggcgca tcactgtgga cgaagacttg gtcctgtact tgtttggtac
     1561 acccgggcaa aaacgcttcg actttatgtg ggagattctt tcagaaggaa tgctgggttt
     1621 catcgttatg gtggatagca cccgccctga aactttccgt gaggctcgct cgattctgga
     1681 aaccttccgc gcgtacgccc cgaccccttt tgtcgttgct gccaataagc aggatgtgga
     1741 cgatgcctgg gatcttgaag atgtgcgcca tgctttgcgt cttgatggcg atacaaagtt
     1801 actaccctgt gtggcaacga accgcgatac cgtcaaaacg gttttgctgg agttgttata
     1861 tagcatctta aaagaaatgg actccgaaaa atagggtcag gttgtatcct tgctgtgtct
     1921 tcaattacta ctgatcaggg aatagttcat tatgaagtat acgggcgggg gcgcccggta
     1981 attttgctgc atggctatca aggctcatgg ggattgtggc agcaaactat ggggtatttg
     2041 ggtgcttttt atcgcactta tgccttggat ttttggggat ttggcgaatc tggtacaaaa
     2101 cgtgagacat acgctgtaaa agattttgtt ggcttggtca accaattcat ggagcaattg
     2161 ggaattatcc gtgctccgtt ggttgggcat agcatgggtg gtaccgtcag tttattgacg
     2221 gccatgcaat acccggaccg ggttgaaaaa gttgtggtaa ttggctcacc tatttcaggt
     2281 tcctctttgt attttttccc gcgcgtgttc ggatatcgtg catttggctg gctgacctac
     2341 cataatcttt ggatctacaa gtctttttac cgcatccttg caccatatta ttccaaggat
     2401 aagaattggg ctgccatgat ggaccgggat gtatctcgaa caacgcttga agcctttttt
     2461 gcaagcattg gctcgctcag aagaacggat ttacgcccca gcctccatac cattcaagtc
     2521 cctgtaatgg gtatgtatgg agacaatgat attgtggtaa acccaaatca atggaagcca
     2581 ttgcaagaag gtgttgcaaa gtctcatatt gagcgtttcc caaaagccgg gcatttcatc
     2641 atgctggatg agccgctaga attcacccaa aaactaaaag cattcctaga ccaagagaat
     2701 cccgcttcat gagtccgcag aaatactatc ctcagaaaat ggggagaatc atcctgcaag
     2761 gcatggaaga agtaatgggt gtgaacgggg tggaaacaac ccttcatctt ggatcccttg
     2821 accacattat ccaaaacagg ccaacagcta cgtcagaccg cacttttcct tttgaggtgg
     2881 tcagtgccat tttagcgtct cttgaaatgg tttacggtcc acatggtgga aggggagtgg
     2941 cgctgcgtgt aggccgatcc actttccgat atggattaaa ggaatacggg tcaatgcttg
     3001 gattgaccga gatggcattt cgccttttgc ctcttcgcac caaactgcgc acaggtgcga
     3061 gagtgtttgc tgagcttttc aacaagcaca ccgaccaaaa agtgcgcgtg gaagaagtag
     3121 agaataagat ctattggatc atcgaaagct gtccgctttg ttgggataga aaagcggatg
     3181 aagcaatttg tcatcttgca gtcggcgttt tgcaggaggc tttgtattgg ttaagtgggg
     3241 gtaaagtgtt tagtgtggaa gaaacagagt gtattgcacg aggcaatgcg acctgtacca
     3301 ttgtgatcga ccaaatcccg ctttcataac aaaataatat ctgattttaa cagttttgtt
     3361 tgacgggcat tttgcctcat actttgattt ataatcatac attatgctta aagttgtcct
     3421 gcatgcaccc aatccgatca tgccgttttg tgaaccagca cgcgaattgc gtatccaaaa
     3481 ctcgcctctt tggttgcacc agcgcaactt attggctcca tatgtaacgc gtgaaatgga
     3541 acttaaacaa ggggaacgtt tacagcccgt ccgtgaacaa gccatagttt accgggataa
     3601 cctcttcttt gataaatact atatccaggc tttcatggat gaagccatcc gtctcaacag
     3661 acctgtgcga gctgctttcc gctccgacga ccctgctttc cgtgaacatg ccctgccgct
     3721 ttccacttca tatacccctg caggaagttt gtatcttgcg gatctgtggt attaccctaa
     3781 tggcccggta gccggggcag agccgcttgt cattgacatg caggcacggg aaattgggta
     3841 ttaccatgta ccaacctaca tgggagaccg cagtggcgac cttgtttttc aggtgccttt
     3901 gcgtgccatg atggccattg attcatgggt gcatgttttt gttgcagata tggtatttgg
     3961 tcagtttgcc cacggtgccc gttttgagaa gcgcctaaac gaagaacttg gatttaagct
     4021 gaagatcatt ggtaaagcca tttatgaggg caggcaggtc ctcgaatgtt cagagttggt
     4081 aaaggtgggt aaggggtgcg tcattgatcc cagcgccgtt attcatggac cggctatcat
     4141 tggcgataat gtaaccatca atgccggtgc ggtcattgaa aacagtatca ttggaaacaa
     4201 tgtaaatatc tcacaggatg tacaggtaat gctttcagtg gttggagatg gggccttcct
     4261 gccctttcgt gcggcattgt tcatgaccac cattatggaa aacagcatgt tggcacaaaa
     4321 tacatgcttg caaatgtgtg ttgttggcag aaatacattc attggcgcgg gttctacatg
     4381 gacagattac aaccttatcc ctgcccccat tcgtgctcgt gatggtaatg gaaaattaag
     4441 cctgtccaac cggcctgtga tgggtggttg tgtaggccat aactgccgta tcggttcggg
     4501 gatgatcgtt tatccggccc gtaccattga atcggatgtt gtgttggcgg cttccaagga
     4561 acgccgtgtc attgaccggg atatcgcgtt cgacgatagc gatcaccacc atcttaaact
     4621 tgcccatctt caccaaaccc cataccataa gcaatcaaaa ggtgactcag agtcgtggta
     4681 aaagccgacc gagcatcatc gagacaaaat ggatagtttt gcgttcatca ttcaccccat
     4741 tgaccccaag cgcgatgtca gccgaaaatt cccttttcta ggccgagtat tgactgagag
     4801 gcagatcgac ttcttttcca ccttttttcc gcctgtttac atctcagaga tcgaggggat
     4861 cacctctcaa tccagcggaa aaaccattaa aggctggttc cttgcatgtc cgtatactcc
     4921 aagacgcatg atggagttgc ctgagcgcac agtctatcgt aaaattatcc aaacaggccg
     4981 aatggcagaa aaacttggag ccaatattct cggccttggt gccttcactt ctgtggtggg
     5041 ggatgcgggt gtgaccattg caaacaagct ggatatcccc gtaaccacag gcgattcctt
     5101 tactgtagcc atggcggtgc aagccattcg ggaagccgca caagtgatgg atattaaaat
     5161 ggaatccgcc acggtggcag tggttggtgc gacgggtgca attggccgcg tctgcgctga
     5221 actgttggct ggtgaagcgg aacgggtttt actcgtggcc cgggatgaga aaaaactcga
     5281 agttttgcgt gacaggctca aggtccaggc aagcagcgag ttatgtatca gcacaaaaat
     5341 ggatgttttg aaggacgcag acttgatctt aacggttacc agttccattc atgacgtcat
     5401 ccgcccggag catttgaagc cgggaagtgt ggtttgtgat gtggcacgcc cccgtgatgt
     5461 ttctgcaatg gtggcagcgg tgagagatga tatattagtg attgacggtg gaatggttga
     5521 tgtgccagga gccgtggatt tccatttcaa cttcggtttt ccagatggca aggcatatgc
     5581 ctgcatggct gagaccattg ctttggcttt ggaaggccgt tttgaagact atacagttgg
     5641 caaggatatt acgttggaac gagttcaaga tatcactgcg attgctgaaa agcatggttt
     5701 tcgtatgagt ggattcagat cgttcgagcg cgaagtaacc atagaacaga ttgaagccgt
     5761 tcgacaaaat gcccgcaaag ggttacgacg tgcttaaagg aggctcaaca tgggaaacgc
     5821 ccgacttttg gtagtggaag atgatgttga tatcgcgaat atgctcaaga tctacttcac
     5881 aagtctgcaa tatgacgtgg atgtagcgaa tcgtggccgc gatgcactgg ataagacaaa
     5941 acaggttttg ccgcacttga tcgtgctgga tatcatgttg ccagatattg atgggtatga
     6001 agtctgtcgt aatctgcgca ccagtacacg taccagccac atcccggtaa tcttcctgac
     6061 acaaaaagat gagcgcagtg ataagttgca aggccttgaa ttgggcgcag atgattacat
     6121 tacaaagccc tttgacattg aagaattaaa actgcgtgtg cagggtgcca ttcggcgctc
     6181 tgaacgcgaa agcctcactg acccgcgctc cggcctgcct gccggacggt tgatcgaaga
     6241 acaattacgc cgcattattc gcgaaaaaga ctgggcattg cttgatgtac gtgtcaataa
     6301 ctttgaagca tttaaagatg tatatggttt tgtagcaggc gatgacgttg tgcgctttgc
     6361 tgccatgatg attggtgagg tcgtggatga actggggacg acaagcgatt ttatcggtca
     6421 tgcaggcggt gataatttca tcattatcac aaccaatgat gccgcccccg gcattcgcca
     6481 aaaattaaaa gaccgctttg ccaatgaggt gcaaagccat tacaacttta tcgaccgcca
     6541 gcagggtttc attcaggctc cgaaagctga agggggcgtg gaaaaggttg gcttcatgta
     6601 tctttctgca gggttggtct cgccgagcct tcagtccttc gcggatattc gcgagatcac
     6661 cgaacttgcg gctgaagcac ggcgacagga tacagctgcc acttctgctg cctgataaaa
     6721 gcgagcgttt tcatgtcttc cgggttgggc cttaacctgt taattgccgg gctgattttt
     6781 ggagcactgg tcctggtgat tttgcgggtt ttggctcgta cgccctctgc ggttgcccca
     6841 aacatcgctg cagccaattc ttataccatc cccgaatttt caagatcaaa cgataacgac
     6901 gccattatca tcctcgcgcc cggtgggcgg gtggaatata tcagtgcgct tgcgcgtaca
     6961 tttttcgatc tgcgcgagaa cgagccgtat gaccttgagc gcctttctcg gcgcgcccgt
     7021 ccttcggatg attttcttga cttgtgtgtc tcttcaggat acaagcgcct ttccattaat
     7081 ggcaaacttg tagaagtggc ttcttatgag gttccgggga cattcccggt tatgttgatc
     7141 tcactgcgca gcaaggaatt tgcacccgcc cttgagcaaa gtgacggcgc ttcagaagag
     7201 attttgcggg tggtgactga atttagccaa agcattgcca ccagccttga tcttgaaacc
     7261 acggctcaat ccattctgga taatgttggg cgtcttgtgc cgtcggatgt gcttgaattg
     7321 aaattatgga attctgaaga tcaagatttg gatctttacc gttcccaata tgccaatctg
     7381 gggtccaatg gctcgggacg gatgatgact tcttattttg gaagactgac tcgccagttg
     7441 gttgaaagcc gtacaccgat tctgtttgga gatgttcgtt cccgcccgga tatgaatgca
     7501 aatgaggaag tgcttccgat tcaatcatat ttgggtattc cgcttattgc aggcggggaa
     7561 ctggttggct cacttgaagc tggtcaaacg ggaagcgggg cgtttggcca gtatgatctt
     7621 aatttgctgt tactggtttc aggtcaggcg gcggttgcga ttcgtaatgc aaggctgtac
     7681 gtcaatgagc agaaacgtgc ttctgaattg gcaggccttg caaatttgaa tcaaactctt
     7741 ggggctgtcc gcgatatgca ggagctcttt acaaggctcg tggatagtgt ggctcccttg
     7801 tttcaggctg agattattgg ctttctcctc tacgatgaag aaaaacatac catggaaggt
     7861 aaatctccct tccgcggttt gcctgctcat ctggttgata tttatcgtgt cacagtccct
     7921 gcagaaagcc tcgctgaaaa ggtgattacc ggccagcagc ccatactcac cctgaatgca
     7981 gctggagatg aaaactggcg cgcgcttggt ctttctgata ttgcgatggc tgccagtctg
     8041 cgtgatattg ccctgatgcc tttgttgtct tcaaaccgta tgcttggtta tttgcagatc
     8101 gggcatcata cccgtggggc atccaatttt ggtgtggaag aagtccgttt gatgagtatt
     8161 gtggccaatc aggctgcagc gatcatcgaa aatgtactgc ttgttcagca agcgcgtgga
     8221 cgtgcccaac gttctgatgc actgcgccga attgccagtc tttcaggttc ttcagccaca
     8281 ttggaagaga tcctcaagtt ttcaattcgg gaattatcga atcttttcca ggcggatgca
     8341 ggtgcgatct ttctgatgga tgaggcacgt ggagagttgc gcctgaggcg cgaagctacg
     8401 ttcgggcttc caaaggatat cagcagttct ttcatccaga tattcatgga tgatcccagc
     8461 tatcgttcaa cagtttccgg tagtcagaag cctttcattt cgggtcatct cagtacagat
     8521 cgtcgagtgt tgacggtgta tcgtccattg accactacac tttcgcttga gtcgaccatt
     8581 gttgtgccat tgatcgtgcg cgatcgcagc atcggtgagt tgatgctggg aagtttcaag
     8641 cccgattatt tcaatgccta tgatctgcaa gtgatcgcca cggcagcagg acaattggcc
     8701 accgctgtgg aatcttccag cctgctgaat caaacggatg attcgcttcg tcggcgggtg
     8761 gaccagcttt cggcggtgac aagaatcggc cgtgaattgg gcgcgtcgct tgatatttcg
     8821 catctgatta agaccattca tgaggaaagt ctgcacatcg tacaggcgac ttgttcttct
     8881 gttttcctgt tccgccaaca aggtgaatcc gctggtgcag aaatagaatt ctctgccggg
     8941 tgcggaagcc gtgaattatc gccagccgaa acagatgtgc tgatgactcg ggagccccga
     9001 atcattgctg agttttcgca gaatgaccaa cctcttccgc acgatggcgt gcgttctgct
     9061 ttgatcgttc caatcacgta tcaggcacgc ttaattggtt tgatcagcct gcactcgaac
     9121 aaggtcaacg catattcagc cagtgatatt gagttggttc agattttggc ggtgcaggca
     9181 gggatttcat taagcaatgc gcaacgttat caatctgaga agagaagcgc agagttaatg
     9241 cgccgtcgct cagatacgtt ggtgcgcctg accgacgtaa gttacaacct cggccacgac
     9301 caacccctgg atcaggcttt gcagatcatt gcacgcggca tccgtgagtc gacacctttc
     9361 cgagtggtgt tgatgagcat ggtggaaacc gaaaccggta tgctgcgccg tttgaccgcc
     9421 gttggcattc cgcgcgaaac catgaatgag ttgatggcac gcaaacaacc gttggccagc
     9481 cttcaacagt tgatgcggcc tgaattcaag atcagccgct cctacttcat tccgatcgat
     9541 ctgtctcctg tgatgccttc cgatgtgcat atggttacat tggaagtttc gtcgtctgca
     9601 gaaaagacac ccaattcatg ggatccggac gacgcgcttc ttattccgct cgaaaatgcg
     9661 gaagggcaga tcgttggttt gatcagcctc gatgacccat ccaatggact gcgcccagac
     9721 caggcaacca ttgagtctgt ggaagttttt gccgcccaag ctgcactttt gatcagcaat
     9781 acactacggc agggtgagtt gcgcaatcgc atcgattcgc tgtcggcggg tttgctgcgc
     9841 cagcagaagt tgatcgatat cacccaaaac gatcttccga ttttgcttca taaagacctt
     9901 gagcagacca tttctcttta caacctcgac cagcgtgctc aaagagtgcg tgctggcctt
     9961 gcgatcacca aatccgtcag ccgccagttg gatgcggtgt ctgcgctcaa tgcgctgggc
    10021 cgcgaaacat tgacgcagtt gggcatggcg atcgccctgg tagccgaaaa caccccggac
    10081 ggtccgcgtt tgatgcatgt acttggcagc ctgccgcgct ccaccaatgt ggagtcgttg
    10141 ttcggtcaac gcaacccgct gcgtgcatgc ttgcagacag gaaatccaat tctcatttca
    10201 agccttgatg aaaatgatga atggcgcgat gccaccttgc tcacctccct gcgggcaaaa
    10261 agcgtcatct gtttacccgt ccttgtagaa aacaaaactg tggctgccat gctggctatt
    10321 agtcatgagg cattgcctga tttcaccgat gaagacagca aggtctattt gcagatctcg
    10381 cagcaggcct ctctcatttt gcagaacatt tcattgctca gccagacgcg ccgccgcctt
    10441 gatgaggtga atctgctgct cgatttcagc cgccagcttt cgggtatgga tccagatgcg
    10501 atcgtcagat ccctgctcga tagtgccatc cgcgttctcc aacatgcaca tgcgggtgtg
    10561 gcgctggttt ggaatcaaca gactgaaacc ttggttccgc gcgcggctgc tggttacgct
    10621 gataacacca ccatgttggc catcaactat cggcagggtg aagccctgcc gggcaccgtg
    10681 tttatgaatc gctttgcccg ccgcgtggac gaagtgaatt ttgttcgcga ctacaacctg
    10741 agcacggaaa atctggcttt gtatcgtcag gccacaggtg gacgtttgcc cgtatccagt
    10801 ctgcttgtcc cgatcggtac tgctgaccag aatctgggct tgctggtgtt ggataatttc
    10861 aacgcaacct ctgctttctc acatgaagat gaagccctgc tgctctcgct tacccagcag
    10921 atcgcgctct cccttgataa tgttcgccta gtgcaagcca cacaggaacg cgccgggcag
    10981 ttgcatgcct tgaataatgc cgcggcttca ctcacctcca gcttgcgcag tgaccaattg
    11041 atcaatagcc tgctcgatca actggtcccc atcattcaat acgatacggc cacattgtgg
    11101 ctgcgtgata aggaccgcct cgcagttgtt tccgcgcgcg gtttccccga tacagaagaa
    11161 cgccttggct tatcagtggc cgttcaagac agtgcccttt tcaaggaaat ggcgcagaca
    11221 ggccaaccca ttcttgtaaa tgatgtgcgt gaagattcgc gctttatgcc ggtggatgct
    11281 ccacggcttt catggcttgg cattcccatg atctccaaag gtgaaatggt gggagtgctc
    11341 gccgttgaaa aatggcaggc acactattac acacgtgagc aaattcaagt gagcctgacc
    11401 tttgcaagtc aatcggcggt ttcgctcgat aacgcccgca tgtatgaaga cagcgttagc
    11461 cgtgccacag aactcgacca gcgttcacaa cgccttgcca cgcttaatcg ttttgcctct
    11521 gccctgaccg gcctgctcga tactgaccag attctgaatg tgactgccaa cgaattgctt
    11581 aaagggttgg gcgcaaaacg cgtctctgtt gtgacctttg aacggggtcg ggcgtactgg
    11641 aagatttcct ccccgcgaac aaggatcaaa ctgccgcgcc tgctgcccga ttcacccatc
    11701 ttcagccgcc tgcgcgaatc gctgggtatt ttcaacaccg atgatgcccg caatgaacct
    11761 gatctggttc cgcttgaaga aatgcttggc gacaacccaa ctgcattatt ggtcctcccc
    11821 ctcaccagcg gacaaaacct ctcagccctg atctttgccc aaacatcagg cgatgcgcgc
    11881 tttgggttca acgagcttga aattgcaaag accattacca atcaggccac tgtcgcgctt
    11941 gaaaatgccc gtttgttcca attatctgtc cgtacggcag aacggtttgc catcctcaat
    12001 gaaaccagtt ccctggtaaa ttcaagcctg aatcaagaag atgtatatct ggctgcccgt
    12061 aaagcggcag aacgtttggt gccgttggat tccttcgtca tcaccctgct ggatattgag
    12121 aagaacgaaa tcgaccccgt ttacatgatc gaccgtggaa aacgcatttc tgccgaccgt
    12181 gttccttttg gtcaggggat gagcagtcag gtcatttcaa ccggcaaacc tgttctgatc
    12241 tcaaatagcg aacaggcgaa tgatatcaac accgtactgg ctggcagcag cgatgacgaa
    12301 gaagagaccc gctccattgt ggctgtgcca atgaccttgg gcggaaaaac acttggcatg
    12361 ctctccgctc aaagttatca ggaaggcatg tacaccgagg aagacgtcca gatcctcgga
    12421 acacttgcaa atcaggttgt ggtcgccatt caaaacggac ggctctttgc agaaacacag
    12481 agcctggctt cccagttcga gaaacgcgtt attgagcgta ccgcccaatt gcagcgtgaa
    12541 cagcagaata cagaaaccct tttgcgcatc ctcacagagg tttcctccag tctcgatctg
    12601 gaccgtgccc ttaaccgtac attgtcattg ctaaacgaag ccatcggcgc agaacagggt
    12661 accattatgt tattgcatgc cgaggacaac cttttgcatt accgcgcagg ctatggctac
    12721 ctttcagatc gcaacgatac cagtggacgt ggcttcacct tgagagtcgg tgagggtctg
    12781 gctggctggg tcgtgcaaaa ccgtgaagcc gttttgatcg acgaccttca cgaggatccg
    12841 cgctgggtga gaagctcagc ctccggtatg gatcatcgca gcgccattgt ggcccccatg
    12901 caagtgggtg aggatgtgat cggtgtgctc atggttttcc agcgggccac caatttcttc
    12961 agttcagaaa tgctcaatct ggtccgtgcc attggcgggc aggtggcggt tgcgatcaac
    13021 aatgctcatc tctatgagtt gatccgtgac caggcagaaa gactcggtgt catgctccgc
    13081 aaggaacagg aagatgccag ccgctcacaa gccatcctcg aagctgtggc agatggtgtg
    13141 ctcgttaccg gtgccgataa ccgcatcacc tttgtcaaca cttccactga gcgcatcctt
    13201 gcattggatg aagcccgcct gcttggcaat ccgcttgatg tgtttggtgg tctgttcgga
    13261 aaagccgcca gcacgtggat ggaaaccatc cgccgctggt cggaggatcc gtcttcctat
    13321 caatctggtg actcgtatgc cgagcaattg gaattggaaa atgaacgcat cgcgctcgtc
    13381 caccttgcgc ctgtgatttt gcaaaatgat ttcctcggta ccgtatccat cttccgcgat
    13441 atcacgcatg aagtggaagt ggaccgcctg aaatcggaat ttgtagccac ggtcagccac
    13501 gaactccgca cccccatgac agccatcaaa ggctatgtgg atattctgac catgggtgcg
    13561 gcaggcgctc tcaccgaaaa ccaattgcac ttcctcgata ttgtccgtaa caatatcgaa
    13621 cgtctcaata tcctcgtcaa cgacctgttg gatatttccc gcattgaagc tggacgagtg
    13681 accttgaacc cgcaatccgt caacctgcgc gagatcgccg aagatgtcat cgcagagacg
    13741 ttgcgccgtt cacagaacga aaataaaccc atggccctct cactggatgc cccggcgaag
    13801 atgccccagg tcatgggtga tagcgaccgt gtccgtcaga ttatcggtaa cctggtggat
    13861 aacgcgttca attacacgcc cagcaatggc accatccgcg tgaacattca ccaggaaaaa
    13921 gatgaagttc aggtggatgt gcaggacaac ggtgtgggca ttgcactgca agatcaaggc
    13981 cgtatctttg accgcttctt ccgcggtgaa cacccccttg ttcttgccac acccggaaca
    14041 gggttgggat tacccatcgt tcgccagctg gtcgaaatgc atagcggacg catctggatg
    14101 acaagcacag gtgtgccagg cgaaggcagc acattttcct tcaccctgcc caccgtacat
    14161 aatggagttt gaatggcaag gatcttaata gcagaagacg aaccagatat tcgtgaactt
    14221 gtcgcgttca ccctgcgctt tgcggggcat gaagtgacgg caaccgccaa tggagaagaa
    14281 gccctgcagc aagccgggca gatcattccc gatatcatca tcatggatgt gcgcatgccg
    14341 cgcatgaccg gctacgacgc ctgccgtgcc atgaaagctg accccatttt gaaggatatc
    14401 cctgtggtct ttctttcggc caaaggacag gattcagaga tccaaactgg tctggaagca
    14461 ggcgctgagg aatacctgct caaacccttc gcccccgacc agctttcaga acgtgtaaag
    14521 gcgattcttt cgaaattcgg aaaataacga ttcaatgagc gcaatcattc tcgacggttc
    14581 gatggtgcat tatgaggcat tgggacgcgg acgccccatt gtctttctgc acgattgggt
    14641 cggctcatgg cgatactggg tcaatgccat gcaagtcgca tccacatcgt tccgcgccta
    14701 tgcgctcgac ctctttggtt ttggcgatac ttcacgcgac ccattgcgct attccctcga
    14761 taaacaatcc gaactcgtca atcgcttttt gcaggaaatg ggtatcggca aagtggcatt
    14821 ggtggggcat ggcctcggcg ccctcgttgg ttttacctac ctcaaacaat tcccatcgac
    14881 cgttgaccgt atgatggcag tcaactgtcc gtttgaatat gattcggtca atacccgcct
    14941 gcggaccgct gccatgcctg aactggtcga ttggctggca ggccgcaccc ccgaagctgt
    15001 atccgcactt tccgatgcgg ccaaagccga cccgcaagct gtttctgctt cgatggccag
    15061 tttgcaggga aataactact tcgcggccat tcgtgatctt ggtgttccct gcctgttcgc
    15121 ctatggctcg gaagaccccg ccatcagcat gcccacccca gaccgggtcg attcgcttcc
    15181 aatgcacatg catctggtca cgctcgaaga atcaagtcac ttcccgatgg tggataaccc
    15241 cacccgcttc aaccgcctca tgaccgattt cctcgccctg gattcagggg ccagtccgcg
    15301 cgaattgcaa atgaaagaag aatggaaacg cagggtgcgt taggaagaaa agaaacttga
    15361 attgacatcc cgccctgagt ataaaaaaac agcctcccga aa
//
LOCUS       DAOQJC010000097        15345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1839_length_15345_cov_9.255461, whole genome shotgun sequence.
ACCESSION   DAOQJC010000097 DAOQJC010000000
VERSION     DAOQJC010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15345
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1839_length_15345_cov_9.255461"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PLE39_13820"
     CDS             <1..604
                     /locus_tag="PLE39_13820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69458.1"
                     /translation="SMKTRWIQPVTLTGAHVVLEPLSLEHLDGIKEAVLDGELWKLWF
                     TSIPSPETAETYIRSALELRENAGWMAFVVREKATNKIIGSTRYCNVDEVNQRLEIGY
                     TWYAESYQRSAVNTECKYLLLTHAFEKLDAIAVEFRTHWHNHKSRAAIARLGAKQDGV
                     LRNHTKSANGVYRDTVVFSIINLEWPVVKQSLEYKMSKQY"
     gene            complement(601..1962)
                     /locus_tag="PLE39_13825"
     CDS             complement(601..1962)
                     /locus_tag="PLE39_13825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE69459.1"
                     /translation="MVSTKTVPHTRVKAKIKKDDERLRLALRLSPSPMWIFDLESLLF
                     LAANRPAQLQLEYSLPALKRMKLSDIIPADEHPNLYLTLKQVGRFGHFPGRWHLLPKS
                     GQTISVSVNSSLVKFNGRNAVLVSAFASPFEGLLTENCSRYFALVANRISSAVISTDS
                     ALRIIQWNKAAEDLYGLTETEAMGCLIDEVCCTEFGEDQQSEARASLRTKKRWQGELK
                     QHHRNGAELWVSTSVTLLEDENGNFLGGITINHDITERKKVEDDLRHTKQVMEEINQI
                     LQHAFEREQLASRTDALTGIFNRRYFFEFIEYEVALSKRYQQPFSLIMFDVDHLKLVN
                     DTAGHQAGDEVLKQIVLLVQKELRSVDILARYGGDEFVILLPNSSVPDALILAERIRS
                     QIFAANLRVAGSSLQVTVSIGAADFNPSVSSSAELISHADEALYSAKQAGRNCILAYS
                     AKI"
     gene            complement(2071..>2709)
                     /locus_tag="PLE39_13830"
     CDS             complement(2071..>2709)
                     /locus_tag="PLE39_13830"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE69460.1"
                     /translation="LGIRGSNRNITARKQAEEDLHRTKKSLEEVNSVLLAAIEREQRI
                     SRTDSLTETYNRRYFFEIATHECSVAERYRQPLSLIMFDIDNFKKFNDIYGHQVGDEV
                     LRRIAQVAREQLRSADILARYGGEEFTVLLPKSNTHEALIVAERIREKVAACKLDTGN
                     GVVGITISIGVAEYFPDQQTLDDLIRNSDKALYTAKEKGRNQVSVYATGNTV"
     assembly_gap    2710..2809
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2810..4701)
                     /locus_tag="PLE39_13835"
     CDS             complement(<2810..4701)
                     /locus_tag="PLE39_13835"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE69461.1"
                     /translation="MSLKNSPQKVNILKGRDSAEFRKMFGNHPTPVWIHDRESFAILA
                     VNQALNSLLGYEPTELEGISLENIFPVEEVSSLYADLKLPRQIGHSPREWHLRSKSGQ
                     LISIDQTFSSLIYEGREAALVIVHLRTDQTLESRILGSRLRISEFAHSHSLDDLLQKT
                     LDEAELLTQSNIGFFHFVEKDQKTLSLQNWSTNTLKNMCTAEGKWSHYSIDEAGVWVD
                     CVRFGGPVIYNDYPGLEHRKGLPPGHAPISRLLSVPVTRGGSVVAILGVGNKVEEYNE
                     SDIASVYLLADLAWDITVGKRTEHGLHQSEERLRLLFDKISNIAVQGYAPDGTVRLWN
                     QASEKIYGYTKDEALGRNLLDLIIPEEMRADVEASVKKMIGTMQPIPSEELSLKRKDG
                     SRVSVYSNHTVVEISGVGRELYCIDIDLSERKSFEEALRESEEKYRQLFEAESDAIFL
                     IDNETGQIFEVNSAATKIYGYTREELIRMRNVDLSAEPDNTRKAMQSKMVNIPVRWHR
                     KKNGVVFPVEISASHLMWKGRPIHIAAIRDITERKQAEDALHESEAQYRLMFQNNPHP
                     MWVYDLNTLKFLEVNDAAISHYGYSRDEFLRMTILEVQSVDSLAMLRAPLVDPFLQDY
                     RSGVWQ"
     gene            complement(4852..5736)
                     /locus_tag="PLE39_13840"
     CDS             complement(4852..5736)
                     /locus_tag="PLE39_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69462.1"
                     /translation="MSESTNQTGGNRLSPKTILRLYMAVSFFILLLILWTYYAEPLTE
                     SVSAYWNDKLLDTLILIPALGAAIAGVLVVRQFDAGEQPHRIWQAFTIGLWFWVAGEL
                     SGFVYDAIYIDLPYPEFSLIDVCWLAGYFYLGLSLYYQVRLVYSAQKRKGRLLYLALV
                     GLAFLIAALLTQLAAKAGLGGESAWIILFVSILYPVFDLAEGSAAIWLSLLFGRGQWS
                     RPWWGLILFALADSVDTFYWLGGYDFIAPVVQSVLDVISVAAYPASYMVAGLALLSNY
                     FILQYGVESGLLQTQKRK"
     gene            complement(5741..6667)
                     /locus_tag="PLE39_13845"
     CDS             complement(5741..6667)
                     /locus_tag="PLE39_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69463.1"
                     /translation="MKHFFDQAQRLPEDTSQNAVESFFRGQNASGLIEKQLHSGKSVI
                     LLFANGSPSAAYQLENGLGTFITHGEFSAVQNEIANVRALQLPDVAGRLTLLALESQI
                     GDELAMPDSVIWQAQLEKWKQARWSGLVEVESNDLQGLALFWQGELQASDVIFSTASG
                     FVTELPKAGIFPWKVTTYLPVPTAQGYQCAILRLGAVHWSFRILSRYQEMVGQKLLQT
                     MDRELNRQIQPWRWKIALTDSNMLDTHFFPYLMDAAHAYRALFMAMGAQMNFVIGSNL
                     TLRLLNETFEQIHPDERALLQSQRLIPAAFSE"
     gene            6860..7432
                     /locus_tag="PLE39_13850"
     CDS             6860..7432
                     /locus_tag="PLE39_13850"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNE69464.1"
                     /translation="MTTLAPTLSIRYFPRTATWLRDLLLIVFGSLFVAALAQVEIPLP
                     FTPVPITGQTFGVLIIGAAMGSRRGAMSLALYLAEGIVGLPFFAGGAHGLSVVVGATG
                     GYLIGFIAAAYIIGLLAERGLERNARTSFIPFLVGTTIIYAFGITGLSILLGSFSKAV
                     ALGMVPFLIGDVIKLLAAAVALPAAWKIVK"
     gene            complement(7502..8311)
                     /locus_tag="PLE39_13855"
     CDS             complement(7502..8311)
                     /locus_tag="PLE39_13855"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNE69465.1"
                     /translation="MEQLKLLAVFAHPDDESMGMGGTLAKYSAEGVDTHLVCASRGEV
                     GWFGSEEQNPGPERLGQLREKELSNAVHELGMKSLHFLNYIDGSVDKADHAEAIGRIV
                     THIRRIKPQVMVTFPPDGNYGHPDHIAVGQFTSAAIVCAADASYVDAENLPPHRVSKL
                     YYMVDGENFINLIAPFMGDMTFPVDDQIRGEIAWKEWMITTRIEMSAHCNAAWKAIQC
                     HQSQLPTLGALAEMDADAAAAVLAMQGAFYRVFSLVNGGRGLETDLFEGLR"
     gene            complement(8363..8836)
                     /locus_tag="PLE39_13860"
     CDS             complement(8363..8836)
                     /locus_tag="PLE39_13860"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNE69466.1"
                     /translation="MDVQSEKLLVRILSTTRIAALGTIRDEEPRVSMVAYVAAHDYSA
                     FYVHVSRLAQHTVDMQKNKQVSLLISETDDGRADPQTLARLSIRASAEFMENGEPGYA
                     PVKKMYIEAFPESEPLFKMADFGLWRIKPKGARYVAGLAKAFNLTPETLQKVAAK"
     gene            complement(8843..9499)
                     /locus_tag="PLE39_13865"
     CDS             complement(8843..9499)
                     /locus_tag="PLE39_13865"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNE69467.1"
                     /translation="MDHEDEFPILVAQEGPLKGQRWSLSRALMVGRDPTCDINVQDRQ
                     VSRFHARITPTTEGVTVEDLGSKNGTNHNGVELTGPVMLQDGDLLGIAIAQQFLFLTS
                     DATMPLAESGARPGRLMMDNKSRRVWVNQQQVTPPLSAQQFKLLWLLYENQGQVISRT
                     DLVATVWGEDQMAGVSDQALDALIRRLRDRLASLDPTHQYIDSVRGHGVRLDNPPSGG
                     "
     gene            9826..10815
                     /locus_tag="PLE39_13870"
     CDS             9826..10815
                     /locus_tag="PLE39_13870"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNE69468.1"
                     /translation="MPQSLQNGEVLRDRYMIREQIGQGGTGSIYLAEDNRLEGRLCAI
                     KEVEHNRTLPTQFLEQAREQFFREASVLARLDHPNLPKVSDFFSEGPRDYLVMDYVPG
                     KDLRERMLDARRNKAFLSENEVLRWAVQISDALNYLHQQEPPIIHRDIKPSNLKITPS
                     GLIKLVDFGLVKILAPNEVTITIIQGQGTALYTPLEQYGGDDSHTDIRADIFSFGATL
                     YHLVTNEPPTEARKRFLNSRSLTPPREINPTLSSRVEKAILWAMSLHPDDRPKDVIEF
                     REALLGKKEAPDHITSSRSSFEIPSFSLFPQEQLATYTAMGLFVLGLIVTILR"
     gene            complement(10863..11804)
                     /locus_tag="PLE39_13875"
     CDS             complement(10863..11804)
                     /locus_tag="PLE39_13875"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE69469.1"
                     /translation="MKCKKCGEKAAVQMRQHKLALCKDHYLEWVPEQTERFIKKYEMF
                     SRDEKILVAVSGGKDSLSLWDILVRLGYQADGLYLGLGIDGGINYSHESQRLSEEFAQ
                     RNNLKLHVVDIEKEYGQSIPVLAQNSHRGYGRPCAVCGLAKRHEMNRVARDLGYDVLA
                     TGHNLDDEAAVLFGNTLHWASDYLLRQGPVLPGSDGLARKVKPLCRFYEREMTAYAIA
                     RGIEYIYEECPFAEGSQSIFYKESLNLLETARPGAKLTFYLSFLEAKKSGNLFIEKNE
                     SFKNVHTCPKCGQPTTTPDFCSFCRMIEKSAATEEME"
     gene            complement(11807..12010)
                     /locus_tag="PLE39_13880"
     CDS             complement(11807..12010)
                     /locus_tag="PLE39_13880"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNE69470.1"
                     /translation="MSQMIAKLILRNKVYEVKAGMALVDALKKNNIVPESVIATRNGD
                     LLTDDEILRPGDEIKLIAVISGG"
     gene            12088..13290
                     /locus_tag="PLE39_13885"
     CDS             12088..13290
                     /locus_tag="PLE39_13885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE69471.1"
                     /translation="MRIGMMVDSYKPYVSGITNYVEVNKKYLEQAGHEVYVFTFGDLE
                     HQPDEPRVIYSKGLPLSDSGFYLSTRYSRTAKQLLQTMDVVHVHHPFLSGRLALRYCR
                     LLKIPIVYTNHTRYDLYAQTYLPIMPEEMSQGILQAYLPSFCNAVDLVVAPSAGMEKV
                     LRQLNVESKVEVVPNGVDLKNFQNANPLARAELGYKPDDILLVYAGRIALEKNLPFLL
                     KSFAGVAQAIPNAQLLLIGSGIQPYEEEIRNLIEEMNLTRRVRMVGRVAYEKLPAYLA
                     MCDAFVTASVTEVHPLSVIEAMGAGLPVMGIHSVGVGDTVVDGVTGLLATHDLPAYTA
                     KLTRLCLDPNLRTQLSDSARKASSAYAIERTTDMMLKHYERLVRESHPRKGHWSVRLR
                     KLLERITQ"
     gene            13287..13643
                     /locus_tag="PLE39_13890"
     CDS             13287..13643
                     /locus_tag="PLE39_13890"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNE69472.1"
                     /translation="MTKHNQEIGAWGEGVAAEWLAAHGREVVARNIRTPYGEIDIVAR
                     QGDVTLFVEVKTLTSSANFFPEAQITARKREHMLNAAQHYCAEHEIDHWQIDVIAVEG
                     KPGSNPVVTHFENVVG"
     gene            complement(13749..14369)
                     /locus_tag="PLE39_13895"
     CDS             complement(13749..14369)
                     /locus_tag="PLE39_13895"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HNE69473.1"
                     /translation="MHIPKLYREEDRELIVEFLKQNNFPALVTFDGEKPIATHLPVEI
                     TQTETAGLTIYSHMSKANPQWKSFGGQEVLLIFQGAHTYISPRWYNHVNVPTWNYMMV
                     HVYGKVRIVEGDELYSLLGRLVRQHEVDSNYRLESLPQDFVKKEMNGTVGFVVDVTRM
                     DAGYKLSQNRNDEDHANIIRELEGRGDVNSMEVAEEMRKRKKRDER"
     gene            complement(14374..14793)
                     /locus_tag="PLE39_13900"
     CDS             complement(14374..14793)
                     /locus_tag="PLE39_13900"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE69474.1"
                     /translation="MSKTIDYILDAFAFMAYLENEPGAERVETILQDVEVGKARAYLS
                     IINLGEVIYTTERKYGLSKAQDTLALIQRMPIEILPADNQTVLAAAHIKANYPLSYAD
                     AFVVIAAQKLDGIIMTGDPEFQDVTELAKIEWLKQRR"
     gene            complement(14786..15034)
                     /locus_tag="PLE39_13905"
     CDS             complement(14786..15034)
                     /locus_tag="PLE39_13905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNE69475.1"
                     /translation="MVLTISNKGWVVIPAELRKKYNLHAGSEVVIVDYGGVLAIIPAA
                     RYPVKQGRGFLAGASSASTDLKKDRKLEKKRDARLANE"
BASE COUNT         3738 a         3872 c         3837 g         3798 t
ORIGIN      
        1 atcaatgaaa actcgctgga ttcaacctgt tacactcacg ggagctcatg ttgttctaga
       61 gcctctttct cttgagcatt tggatggaat caaagaagca gtactggatg gggaactttg
      121 gaaactttgg ttcacttcca ttccatcgcc agaaacggca gagacctaca tccgctcggc
      181 gttggagttg cgcgagaatg cgggatggat ggcgttcgtt gtccgcgaga aggcgacgaa
      241 caagatcatt ggcagcaccc gctactgcaa cgtggacgag gtcaaccagc gtttggagat
      301 cggctacacc tggtatgcag aaagttatca gcgttcggcg gtcaacaccg agtgcaagta
      361 tctgctcctc actcacgcct ttgaaaaact ggatgccatt gcggtggaat tccgcactca
      421 ttggcacaat cacaaatccc gcgcggcgat tgcgcggctg ggggcaaaac aggatggtgt
      481 gctgaggaat cacaccaaat ctgcaaacgg ggtgtaccgc gacaccgttg tattttccat
      541 tattaatttg gaatggcccg tggtgaaaca gtcgctggaa tacaaaatga gtaagcagta
      601 ttaaattttt gccgaatagg caaggatgca attccgcccc gcttgttttg cagaatataa
      661 agcctcatca gcatggctga tcaattcagc cgaactcgac accgagggat tgaaatcagc
      721 cgctccaata ctgacagtca cctgcaaact cgatccagcg acccgtaaat tggcggcaaa
      781 aatctggctg cggattctct ctgccagaat cagcgcatct ggcacgctgc tgtttggcag
      841 caaaatgacg aattcgtcgc cgccgtaacg agccaaaata tcaacactgc gcagttcctt
      901 ctgtactaga aggacaattt gtttcaggac ttcatcgcca gcttgatgcc ccgccgtatc
      961 attcacaagt ttgagatggt ccacatcgaa catgatgagg gaaaagggct gctggtatcg
     1021 tttggataac gcaacctcgt attcgatgaa ttcaaaaaaa tagcggcgat taaaaatacc
     1081 agtcagggca tcggtacggg aggcaagctg ctcgcgctca aaggcatgct gcaagatctg
     1141 gttgatttcc tccatgacct gttttgtatg ccgcagatca tcctccactt tttttctttc
     1201 ggtgatgtcg tgattaatgg tgatcccgcc aagaaaattc ccgttttcgt cttccagcaa
     1261 ggtcacagaa gtggagaccc acaattctgc tccattgcga tgatgctgtt tcaattcgcc
     1321 ctgccagcgt tttttcgtgc gaaggctggc acgagcttcg gattgttggt cttcgccgaa
     1381 ctcagtgcaa caaacctcat ctattaaaca ccccatggct tcggtttctg tcagtccata
     1441 gaggtcttca gcggccttgt tccactggat gatacgcaaa gcagaatcag tggaaataac
     1501 cgcgcttgaa atcctgttcg caacaagggc aaaatagcgc gaacaatttt ctgttaaaag
     1561 accttcgaaa ggtgatgcga aagctgagac aaggactgcg ttccgcccat tgaattttac
     1621 aaggcttgaa ttcacagaga cgctgatcgt ttgtccgctt ttggggagga gatgccaacg
     1681 gccggggaaa tgcccaaaac ggccgacctg tttcaaggtt aaataaagat tcgggtgttc
     1741 atcggctggg atgatatcac tgagcttcat tcgcttcagg gcaggcaggc tgtattccag
     1801 ctgaagttgg gcaggtcgat tggctgcaag gaacaataat gactctaagt caaaaatcca
     1861 cattggggat ggcgacagcc tcagagcaag acgcagcctt tcgtcatctt tttttatttt
     1921 cgcttttacg cgggtgtgtg gtacggtttt cgttgacacc atcctgattt tctttcatgt
     1981 gggtgccata ttgtataacc ccatactgca aaagagggcg ttcactgcca caattcttca
     2041 aactggaatg tattgaattt gtttggaaga ttataccgta ttgcctgttg cataaacaga
     2101 aacctgattc cgacccttct cctttgctgt gtataaagcc ttgtcggaat ttcgaatgag
     2161 gtcgtcgagt gtttgttggt cagggaagta ttcggccacc ccaatgctga tggtgatgcc
     2221 aaccactccg tttccagtat ccagtttaca ggcggcaacc ttttcgcgga ttctttctgc
     2281 cacgatcaga gcctcatgcg tattgctttt tggaagcagt acagtaaatt cttcgccgcc
     2341 ataacgcgca aggatatctg ctgaccgcaa ttgttcacgg gcaacctggg cgatgcgcct
     2401 caagacctca tcccctacct gatgcccata aatgtcattg aattttttga agttatcaat
     2461 atcgaacatg atgagggaga gcggctgccg gtacctttcc gccacggaac attcatgcgt
     2521 ggcgatctca aaaaaataac ggcggttgta tgtttcagtc aggctatcgg tgcgggagat
     2581 gcgctgctcg cgctcaatgg cagccagcaa cacgctattg acctcttcga gagacttttt
     2641 tgtccgatgc aggtcttctt cagcttgttt acgggcagtg atgttgcgat tgcttccgcg
     2701 gatgccgagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gctgccaaac
     2821 tccagaacgg taatcctgca ggaagggatc gaccaaaggt gcccgcagca tggccagact
     2881 atccacagat tgcacctcca aaatcgtcat tcgcaggaat tcatcacgcg aatacccata
     2941 atgtgaaatg gcagcatcgt tgacctcaag gaatttgagg gtgttcaggt cgtagaccca
     3001 catggggtgt ggattatttt ggaacatgag gcggtactgg gcttccgatt cgtgcaaggc
     3061 atcttcggcc tgttttcgtt ctgtaatatc acggatggct gcgatgtgga ttgggcgtcc
     3121 tttccacatc aaatggctgg cactgatctc caccgggaac acaacaccgt tctttttgcg
     3181 gtgccaacgg acaggaatat tcaccatttt ggactgcatg gctttgcggg tgttatcggg
     3241 ttcagccgaa agatccacgt tacgcatgcg aatcagttct tcacgggtat agccgtatat
     3301 ttttgtcgct gccgaattca cttcgaagat ctgcccggtt tcgttatcga tcaggaaaat
     3361 cgcatcggat tcggcttcaa agagctggcg atatttttcc tcgctttcac gcagggcctc
     3421 ttcaaatgat tttctttcag agaggtcaat gtcaatacaa tacaactccc tgccaacacc
     3481 tgaaatctca acgaccgtgt ggttggaata gactgaaacc cgcgagccgt ctttgcgctt
     3541 tagcgacagt tcttcagatg gaatgggctg catggtccct atcatttttt tgacagaggc
     3601 ttcgacatcg gcccgcattt cttcagggat gatcaggtcg agcaggttcc ttccaagcgc
     3661 ttcatctttt gtgtaaccgt aaatcttctc gctggcttga ttccacaacc gtacagttcc
     3721 atcgggggca tatccctgca cagcgatatt ggagatttta tcgaacaaca gcctgagtct
     3781 ttcttcgctc tggtgcaaac catgctcagt gcgcttccct actgtgatat cccacgccag
     3841 gtcggcaagt aaatacaccg atgcgatatc tgattcatta tattcctcca ctttatttcc
     3901 cacccccaaa attgctacaa cgctcccgcc gcgtgttact gggacggata acaatcgtga
     3961 gatgggagca tgccccggtg gaaggccttt gcgatgttca agccctgggt aatcgttata
     4021 gatgacaggg ccgccaaatc gaacacagtc cacccatacg cctgcttcgt caatggaata
     4081 atgagaccat tttccctcgg cggtacacat gttcttcaag gtgttggtcg accaattttg
     4141 aagcgaaagg gttttttgat ctttttcaac aaaatgaaaa aagccgatat tgctttgggt
     4201 caacaattcg gcttcatcca atgttttttg gagcaggtca tcgagagaat gtgagtgagc
     4261 gaattcgctg attcgcagac gtgagccaag aatacggctt tcaagggtct ggtcggttct
     4321 taggtggaca atgaccaggg cagcttcacg accttcataa atgagactgc tgaaagtttg
     4381 gtcgatgctg atcaattgtc cgctcttgct gcggagatgc cattcgcgtg gagaatgccc
     4441 aatttgtcga ggcagtttca ggtcggcgta aagagacgag acttcttcca caggaaagat
     4501 attttcaagc gagatcccct ccagttcggt tggttcgtat cccagcaggg aatttagggc
     4561 ttgatttaca gccaggatgg caaacgactc gcgatcatga atccagacag gcgtggggtg
     4621 gttaccgaac attttccgaa actcagccga atccctgccc tttagaatgt ttactttctg
     4681 tggagaattt ttcaagctca tgattgtgcc ttccgaaagt tgggatactt taccaaacta
     4741 gagaatagat ggattggcag acacaattgc cccaaaatat cgcctcccat cgcgcacagc
     4801 tttttatata actagtttag cccctgccat tgagacgtca agtgacaaac gttacttcct
     4861 cttttgggtc tgcaaaaggc cgctttcgac accgtattgc aggatgaaat aattcgacaa
     4921 caaggccagc ccggcaacca tataactggc tgggtacgct gccacggaaa tgacatccag
     4981 aactgattga acaacagggg cgatgaaatc ataaccgcca agccaataaa aggtatccac
     5041 gctatcggcc agcgcaaaga ggatcagccc ccaccacgga cggctccatt gtccgcgccc
     5101 gaaaaggagg gaaagccaga tcgcagccga gccttcggca aggtcgaaga cgggatacaa
     5161 aatggagacg aatagaatga tccacgcgga ttcgcctccc agcccggctt tggcggctaa
     5221 ttgggtaagc agggcggcga tcaggaatgc caggcccacc aatgccagat acaacaaccg
     5281 tcctttgcgt ttttgggcgc tgtacaccag acgaacctga taataaaggg acaatccgag
     5341 ataaaaatac cctgccagcc agcagacatc aatgagacta aattcaggat aaggaagatc
     5401 tatgtagatc gcgtcgtaca caaacccgct caactcacct gccacccaaa accacagacc
     5461 gatggtaaag gcctgccaga ttcgatgggg ttgttcgcct gcatcaaatt gacggacaac
     5521 aagcacacct gcgatggcgg cgcccaacgc gggaattaag attagcgtat caagaagctt
     5581 gtcgttccag taagctgaaa ctgattcggt aagcggctcg gcatagtagg tccacaggat
     5641 gaggagcagg atgaaaaaag agaccgccat gtaaagacgg aggatcgttt tgggtgaaag
     5701 gcggttgccg ccagtttggt tggtagattc gctcatgagg tcattccgaa aatgcagctg
     5761 ggatcaggcg ctgagattgc aatagcgccc gttcatcggg gtgaatctgt tcaaaggttt
     5821 cgttcaacag acgcagggtg aggttactac caatgacaaa attcatttgt gcgcccatcg
     5881 ccatgaaaag ggcgcggtag gcatgggcgg catccattaa atatggaaag aagtgcgtat
     5941 ccagcatgtt gctgtcggtt aaggcaattt tccagcgcca gggctggatc tggcgattca
     6001 attcacggtc catggtttgc agtagttttt ggccgaccat ctcttgatag cggctgagaa
     6061 tccgaaaact ccaatgcaca gccccgaggc gcaggatcgc acattgataa ccctgggcag
     6121 taggtacagg caaataggtt gtgactttcc atggaaaaat ccccgccttc gggagttctg
     6181 ttacgaatcc gctggctgtt gaaaaaatta catcagacgc ttgcaattca ccctgccaaa
     6241 acaaagccag cccttgcagg tcattggatt ctacttccac caaaccactc cagcgtgcct
     6301 gcttccattt ttcaagttgg gcctgccaga tcaccgaatc gggcatggca agctcatcgc
     6361 cgatctgaga ctcaagcgcc aacagcgtca ggcgtccagc cacatcgggc aattgcaggg
     6421 cgcggacatt ggcaatttca ttttgcacag cagagaattc gccatgtgta atgaaggttc
     6481 caagtccgtt ttcgagctga taggctgcac tgggcgaacc atttgcaaaa agaagaatta
     6541 cagacttacc agaatgtaat tgtttttcga tcaagccgct ggcattttgc ccgcgaaaaa
     6601 aactctccac tgcgttctga gaggtatctt cgggaagtct ttgtgcctga tcaaagaaat
     6661 gtttcattcg agaatagagg taaaaaacag gggaaggaga atggaaacaa cttttgttat
     6721 tttacttgat ttcaaccctg aacaaaggta acaaaagcaa aatgaaagta tgtgtcgttt
     6781 tacctgataa agatggcaga aaacatgacc gttgcaatgt atcaagtaca ctataattta
     6841 taaaaaggaa aaaacactca tgacaactct tgcacccaca ctttccattc ggtattttcc
     6901 acgcacggcc acatggctgc gcgacctgct gcttatcgtt ttcggttccc tgttcgtggc
     6961 ggcgctggca caggttgaaa ttccactgcc atttacgcct gtgccgatca ccggccagac
     7021 ctttggtgtg ctgatcatcg gcgcagccat gggatcaagg cgcggtgcca tgtcgctggc
     7081 tctgtatctt gccgaaggaa tcgttggcct gcccttcttt gctggcggcg cccatggatt
     7141 aagcgtcgtc gttggcgcga caggcggata tttaatcggc ttcatcgctg ctgcgtacat
     7201 cattggcctg ctggcagagc gcggcctgga acgaaatgcg cgcacttcct tcattccgtt
     7261 tttggtgggc acaaccatca tctacgcatt tggcatcacc gggctgtcca ttctgctggg
     7321 aagtttcagc aaagcggtcg cattgggcat ggtcccgttt ttgatcggag atgtgatcaa
     7381 acttctggcg gctgccgtgg ctctgcctgc ggcatggaag atcgtgaagt aattaagtta
     7441 aataaaaaga agaaagagga aagctgttct ttcctctttc ttctttgctt gttcaagcaa
     7501 actatctcaa cccttcaaac aaatccgtct ccaatcccct gccgccgttg accaaactga
     7561 atacccgata aaacgcaccc tgcatggcaa ggaccgctgc cgcggcatcc gcgtccattt
     7621 ctgcgagcgc gccaagtgta ggcaattgac tctgatggca ttgaatggcc ttccatgccg
     7681 cattgcaatg cgctgacatt tcgatgcgcg tggtgatcat ccattccttc caggcgatct
     7741 cgccgcggat ctggtcatcg acggggaagg tcatatcgcc catgaaaggc gcgatcagat
     7801 tgataaagtt ttcgccatcc accatgtaat acagtttcga cacccgatga ggtggcagat
     7861 tctctgcatc aacatagctt gcatctgcgg cacaaacgat ggcggcggag gtgaattgtc
     7921 caacggcaat atgatcggga tggccgtaat ttccatcggg aggaaaggtc accatgacct
     7981 gcggtttgat tctgcggatg tgtgtgacga tgcggccaat ggcttcggca tggtcggcct
     8041 tgtccacact gccatcaatg taattaagaa agtgcagact cttcattccg agttcatgga
     8101 cagcgttgga taattccttt tcacggagtt ggccgagtct ttctggacct ggattctgct
     8161 cttcggaacc gaaccagccc acctcgccgc gtgaagcgca aacaagatgt gtatccactc
     8221 cttcggcaga atactttgcc agtgtgccgc ccatgcccat cgattcgtca tcggggtgtg
     8281 caaaaacggc aagtaatttt agttgttcca tttggactcc tgattcaata aaaattcccg
     8341 caacatgtgc gggattgggg agttattttg cagcgacttt ttgaagcgtt tcaggggtga
     8401 gattgaaagc cttcgcaagc cctgcgacgt atcgtgcgcc cttgggcttg atgcgccaca
     8461 aaccaaaatc tgccatcttg aacagcggtt ctgattcggg gaaggcttcg atgtacattt
     8521 tcttcacggg ggcgtaaccg ggttcgccgt tttccataaa ttccgccgaa gcgcggatgg
     8581 ataaacgcgc cagtgtctgc gggtcggcac ggccgtcatc ggtttcggaa ataagcaggc
     8641 tgacttgctt attcttttgc atatccactg tatgctgggc aagacggctg acatgtacat
     8701 aaaacgccga gtaatcgtgg gcggccacgt aggccaccat cgaaacacgc ggctcctcat
     8761 cgcggatcgt gccgagggct gcaatgcggg ttgtggagag aatacgaaca agtagttttt
     8821 ctgattgaac gtccatgatg atttatccac ctgagggagg gttatctaat cgtacgccat
     8881 gaccgcgtac ggaatcgata tattgatgag ttgggtcgag cgaagcaagg cggtcgcgca
     8941 gacgacggat gagcgcatcc aacgcctgat cggaaacccc cgccatttgg tcttcgcccc
     9001 acacagtggc gacaaggtca gtgcgactga tgacctgtcc ctggttttcg tacaaaagcc
     9061 acaacaactt gaattgctgc gctgaaagcg gcggcgtcac ctgttgctgg ttcacccaca
     9121 cgcggcgcga tttgttgtcc atcatcaggc gcccggggcg ggctccgctt tcggcgaggg
     9181 gcatggtggc atctgaagtg aggaagagaa attgctgggc aatggcgatc ccaagcaggt
     9241 caccatcttg cagcatgacg gggccggtta attcaacgcc gttatgattg gtgccatttt
     9301 tgctgccaag gtcttcaacg gtcacacctt ctgtggtggg cgtgatgcgc gcgtgaaaac
     9361 gcgagacctg gcggtcctgc acgttgatat cgcaggtggg gtcgcgccca accatcaagg
     9421 cgcggctcaa cgaccagcgt tgtcctttca gcgggccttc ctgtgccacc agaatgggaa
     9481 attcatcttc atgatccatg attttgacct caactaggca tattgcaaaa ataaaatttg
     9541 ctgtgcggca tccacaacac gaacacttgt aaagtcattc gtatcatttg ggagttgtat
     9601 ttattttaat ggtttccgac tttgggaaag tcctggattg ccatgtctgc acagtactat
     9661 ataacaacaa agacgtttat aatatagcag aaaagtgtaa ttctgtggat actgcttttc
     9721 tttttttatg gtcttgatat acgcaggtga tatacgccga aaccatcaca ctgccatgac
     9781 caaagcataa acccagagac acacgcttcc cacaaaggag aagatttgcc ccagtcgctt
     9841 caaaatgggg aggttctgcg tgaccgctac atgatccgtg aacagatcgg acagggcggt
     9901 acaggcagta tttatcttgc cgaggacaat cgtcttgaag gcaggttgtg tgcaatcaag
     9961 gaagttgaac acaaccgcac cctccctaca caatttctag aacaagcccg cgaacaattc
    10021 tttcgtgagg catctgttct tgcacgcctt gaccacccga acctgcccaa ggtttcagat
    10081 ttcttctccg aaggcccgcg cgattacctt gtgatggatt atgtgcccgg caaagacctg
    10141 cgcgaacgca tgctggatgc gcgccgcaac aaggccttcc tctctgaaaa tgaagtattg
    10201 cgttgggcgg tgcaaatttc agatgcgttg aattacctgc accagcaaga accgcccatc
    10261 attcatcgtg atatcaaacc cagcaacctc aagatcaccc ccagcggatt gatcaagctg
    10321 gtcgattttg gcctcgtaaa gatcctcgca cccaatgaag tgaccatcac catcattcag
    10381 ggacagggca ccgcgctgta cacgccgctg gagcaatacg gcggagatga ttcacatacc
    10441 gatatccgtg cggatatttt ttcgtttggc gccacactct accacctcgt aacgaacgaa
    10501 ccacccaccg aagcacggaa acgcttcctc aatagccgca gcctgacacc gcctcgagaa
    10561 atcaacccaa ccctcagcag ccgcgtggaa aaagccatcc tgtgggccat gtcgctacac
    10621 cctgatgacc gcccgaagga tgtcattgaa ttccgtgaag ccctgctggg gaaaaaagaa
    10681 gcgcctgacc atataacctc cagccgctcg agttttgaaa ttccctcttt ttcgttattc
    10741 ccgcaagagc aactggccac ctacactgcc atggggttat ttgtgttggg tttgatcgta
    10801 acgatattaa gatagcccac aaactgggtt cgctttactt tccatccaaa tcaggcgggg
    10861 acttattcca tttcttctgt ggcggcgctt ttttcgatca tcctgcaaaa tgagcaaaaa
    10921 tcgggggtcg tggtgggttg accgcatttc gggcaggtgt ggacattttt gaacgattcg
    10981 ttcttttcga tgaacaaatt cccgcttttt ttcgcttcga gaaaactcaa gtagaaagtc
    11041 agcttggcac cggggcgtgc ggtctccaaa agattcagcg actctttgta gaaaatggac
    11101 tgcgaccctt cggcaaaagg acattcttca taaatgtatt cgataccccg cgcaatggcg
    11161 taggcggtca tttcgcgttc gtaaaaacga cacaacggct tgaccttgcg tgccagccca
    11221 tctgaaccag gcaggacggg tccctgccgc aagagatagt cactggccca gtgcagggtg
    11281 ttgccaaata aaacggcggc ttcatcatcc aggttgtgcc cagtggcgag gacatcatag
    11341 ccgagatcac gcgccacgcg attcatttcg tggcgtttgg cgagcccgca cacggcacac
    11401 ggccttccat agccacggtg actgttttga gccagcacag gaatggattg gccgtattcc
    11461 ttttcaatgt ccaccacatg cagtttgagg ttgttgcgct gggcgaattc ctccgacaac
    11521 cgctgggatt cgtgtgagta gttgatgcca ccgtcaatgc cgaggccaag gtacaggcca
    11581 tcggcttgat acccgaggcg cacaagaata tcccacagcg agagcgaatc cttgccgccc
    11641 gaaaccgcca cgaggatctt ttcatcgcgg ctgaacattt catatttttt gatgaagcgt
    11701 tcggtttgct caggcaccca ttcgaggtaa tgatccttgc acaaagccag tttgtgctgg
    11761 cgcatttgaa cagcggcttt ttcaccgcat tttttacatt tcatcgtcaa ccacccgaaa
    11821 tgaccgcaat gagtttgatc tcatcgccgg ggcgcagaat ttcgtcgtcg gtgagcaggt
    11881 cgccgttccg cgtggcaatg accgactcgg ggacgatgtt gttcttcttc aaggcatcca
    11941 ccagcgccat acccgccttc acttcgtaca ctttgtttcg cagaatcaat ttcgcaatca
    12001 tttggctcat aaaacgattt tatcacgcga agcattcccc acttccaatt ccttgacatt
    12061 cattcttcga tgccgtagca tattaacatg cgaatcggaa tgatggttga cagttacaag
    12121 ccgtatgtaa gcggcattac aaattatgtc gaggtcaata aaaagtacct cgaacaggcg
    12181 gggcacgaag tgtacgtctt cacgtttggc gatctggaac atcagccaga tgagccgcgt
    12241 gtgatctaca gcaagggcct gccgctctcg gattctggct tttatttatc cacgcgctac
    12301 tcgcggacgg cgaagcaatt actgcaaacc atggacgtgg tgcatgtgca tcatccgttt
    12361 ttgagcggaa ggctggcact gcgttactgt cgtctgctga agatccccat tgtgtacacc
    12421 aaccatacac gctacgacct gtatgcacag acctacctgc cgatcatgcc tgaagagatg
    12481 agccagggaa ttttgcaagc ctacctgccc tccttctgca atgcagtgga cctcgtggtt
    12541 gcgccttcag cgggaatgga aaaagtgctg cgtcaactca atgtggaaag caaagtggaa
    12601 gtggtgccca acggcgtgga cttaaagaat ttccagaatg ccaatccgct tgcacgggct
    12661 gagcttggat acaaacccga tgacattctg ttggtgtacg cggggcgcat tgcgctggaa
    12721 aagaatctgc ctttcctgct taaatcattt gcgggggttg cgcaggccat ccccaacgca
    12781 caactgctgc ttattggaag cggcatccag ccgtacgaag aagagatccg caatctcatc
    12841 gaagagatga atctgacccg tagagtccgc atggtgggcc gagtggccta cgaaaaactt
    12901 ccagcctatc tcgccatgtg cgatgccttt gtgacggctt cggtgacaga ggtgcatccg
    12961 ctttcggtga tcgaagcaat gggcgcggga ctgcccgtga tggggattca ttcggtgggt
    13021 gttggagata cggtggtgga tggggtgacg ggcctgcttg cgactcatga cctgcctgct
    13081 tacactgcca aactcacccg cctctgcctt gacccgaacc tgcgtacaca actcagcgac
    13141 tcagcgcgaa aggcatcttc ggcctacgcc atcgagcgga ccacggacat gatgctcaaa
    13201 cattatgaaa gactggtgcg tgagtcacat ccgcgcaagg gacattggtc ggtgcgcctg
    13261 cgcaaattgc tggagcggat cacgcaatga cgaagcacaa tcaggagatc ggtgcatggg
    13321 gcgagggcgt tgccgccgag tggctcgctg cacatggacg cgaggttgtt gccagaaata
    13381 tccgcacgcc atacggcgaa attgacattg tcgccagaca gggtgatgtg acacttttcg
    13441 tcgaagtgaa gacgctcacc tccagcgcca atttttttcc cgaagcacag atcacagcgc
    13501 gaaagcgcga acacatgctc aacgccgccc agcattactg cgccgaacat gaaattgacc
    13561 attggcaaat tgatgtgatt gcagtggaag gcaagccagg ctcaaaccca gtggtgactc
    13621 attttgagaa tgtagtgggg tgaggggggg gtagttggcg gtaatcagtt gtcagtggtc
    13681 agtggtcagt gggtggttga cagcgattgg gttagagcaa aaaaacacac agacgaattg
    13741 attttctttc atctttcatc ccttttcttc ctcttcctca tctcctcggc tacttccatt
    13801 gaattcacat cgccccgccc ttccaactcg cggatgatgt tggcgtggtc ttcgtcgttg
    13861 cggttttgac tgagtttata ccctgcatcc atgcgggtga catccacgac aaagccgaca
    13921 gtgccgttca tttccttttt tacgaaatcc tgcggcaggg attcgagccg ataatttgaa
    13981 tccacttcat gttgacgtac caaacggcca agcagggaat acaactcgtc cccttccacg
    14041 atccgcactt tgccgtagac atgcaccatc atgtaattcc acgtcggcac attgacatgg
    14101 ttgtaccagc gcggcgaaat gtaggtgtgc gcgccttgaa agatcaacag cacttcctgc
    14161 ccaccgaacg atttccactg cggattggcc ttcgacatgt ggctgtaaat ggtcagccct
    14221 gcagtttcgg tctgggtaat ttccacgggc aggtgcgtag caatgggctt ttcgccatca
    14281 aaggtgacaa gggcgggaaa gttgttttgc ttgagaaatt cgacgatcaa ttcacgatct
    14341 tcttcgcggt aaagttttgg aatgtgcatg ggattatctt ctctgtttca accattcgat
    14401 ctttgcaagt tcagtaacat cttggaattc tggatcccca gtcataatga tgccatcaag
    14461 tttctgcgca gcaatcacta caaacgcatc ggcatacgaa agtggataat ttgccttaat
    14521 atgtgcagcg gcaagaacag tttggttatc cgctggcagg atttcgattg gcatccgttg
    14581 aattaatgcc aaagtatcct gagccttgga aaggccatat tttctctcag ttgtgtaaat
    14641 gacttcacca agattgatga tcgagagata cgcgcgggct tttccaactt caacatcttg
    14701 aagtatcgtt tccaccctct cagctcctgg ttcattctcc agatatgcca tgaatgcgaa
    14761 ggcatccaat atgtagtcta ttgttttact cattggcgag ccttgcatcc cgctttttct
    14821 cgagtttccg atcctttttc aagtcagtgg aggcagaaga agctccagct aggaaacctc
    14881 ttccctgttt aacagggtac ctggctgcag ggatgattgc caatacgccg ccataatcta
    14941 cgatcaccac ttcagaacca gcatgaaggt tgtatttttt tcgcaattca gcagggatta
    15001 ccacccagcc tttatttgaa atcgtcagta ccataccacg ctccttgtta tacctttacg
    15061 caagaaagta taacacagct aaattacgaa attacacttg cccgcttgag attttggaat
    15121 gtgcatggga ttttttgact ggggcaagta tcgcacggat tttttcaggc tgctgcaagg
    15181 cggggaacga tttcaggtca ggtatacttg ggcatcatag aggagaaatg caccttgttc
    15241 gagcaaacct tcaaaaacat tgacgataag ctttggaaag atgcgggctg ttcaaccccc
    15301 tccgccaccc cctccgccct agcgggcacc tcccccaaat acgac
//
LOCUS       DAOQJC010000098        14971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1900_length_14971_cov_9.776750, whole genome shotgun sequence.
ACCESSION   DAOQJC010000098 DAOQJC010000000
VERSION     DAOQJC010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14971
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1900_length_14971_cov_9.776750"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /gene="uvrC"
                     /locus_tag="PLE39_13910"
     CDS             complement(<1..791)
                     /gene="uvrC"
                     /locus_tag="PLE39_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNE69476.1"
                     /translation="MEISEKLQGILATLPSKPGCYLYRNVEGKIIYVGKAINLKNRVR
                     SYFHADSSHDNKTRRLVRDIVDIEWIVVGSELEALILEMNLIKKHRPKYNIRLKDDKR
                     YPYIKVHWHEPFPRVTVTRNMDEDGSRYFGPYTSAWAVYQTLDVLRKIFPYLTCDREI
                     TGLDKRACLYFDIKLCLAPCIGAVDQAGYRQMISDLMDFLSGHSEEIIKRLEAEMLKA
                     SEEMRFEKAAAVRDQLKAISSIIERQKIIFATDYKDSDVLAMARS"
     gene            990..1295
                     /locus_tag="PLE39_13915"
     CDS             990..1295
                     /locus_tag="PLE39_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HNE69477.1"
                     /translation="MEPSFSNAPIQNGPTGASGGNEKIMAIASLVIGVINLCAWLLPI
                     CGIPLALVGVVLGFLGMKDESQKMLAIAGIGLSAFSLLLACGNAIYGVYVATQGMSF"
     gene            complement(1350..3461)
                     /locus_tag="PLE39_13920"
     CDS             complement(1350..3461)
                     /locus_tag="PLE39_13920"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNE69478.1"
                     /translation="MPSWNGKTLGKVQVGELIARGGMAEVYLGEHTTLNRKVAVKIMR
                     DHVDSDPDNHVRFEREARVVAGLRHPNIIQVYDYELVDGQPCLIMELVTGASMGNYLK
                     ALHKRGEKLPLETVALILSKLSSAVDYAHNQNIIHRDIKPANVLLRSPSSPIDLNKPL
                     PADVEPILTDFGLVRLLDSSIQTSTGTVSGTPAYMSPEQARGEKVNSKTDIYSLGVML
                     YEMLAGVVPFDAESSFGILMKHLNVPPPPIFGISSDLQAIINRALSKDPELRYDSAKA
                     MADEFLAVFNGQTISADTARIAKIPQRVVKPAAEAAAEGKKSTRNNPTLMWVGVALGV
                     VAITALGLVFFKPQTPQATPAPEATLPPPTPTVDPNEILGRVVFTDQNGLMDKAALSF
                     DDLPIPEEGTHYDVWFLGQGGEFVRKAGSITKESLAQGQLVYTNPEQNNVLSLFDQIE
                     ITLEVDNDPNPDTSSGNVVASSVFPPMSLIHVRHVVVSFVTAPEGVALIQGLWGATES
                     LNTTTTELGEAYKAGDETLTRQKLEEIANLIAGNANTVQYIDWDGDGVVSDSGDGFGL
                     LQNGEPGYTDQGYIAQSMSHAQFAARAPDSTANIKLQSANVVICLQNMQGWTEQVLEK
                     VLRLQEMPFGPEMEPIITEILTLSDQIISGVDSNNNQLIEPIVGEGGATTAYEYAYYM
                     AEMPLLLGIHRVPPPAPTPSP"
     gene            3512..3997
                     /locus_tag="PLE39_13925"
     CDS             3512..3997
                     /locus_tag="PLE39_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HNE69479.1"
                     /translation="MTHALEETLREHLRTQGLTVVTAESCTGGLVSDRITNISGSSEY
                     FPGGIVAYSYEAKASLLGVSWDTLNTHGAVSEETVLEMARGVRKLFKSNIGISVSGIA
                     GPTGGTPEKPVGLTWFGLSTDKGEWARHFIWDGDRLQNKHHSSEAALQFVLDYLDGKL
                     E"
     gene            3994..4674
                     /locus_tag="PLE39_13930"
     CDS             3994..4674
                     /locus_tag="PLE39_13930"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HNE69480.1"
                     /translation="MKAVVIVSANAEWRVVKELYPNLEIKNSPYGEYAHLTLGTWNLE
                     LFHGGWGKISAAATAQYVIDHFQPDLLINLGTCGGFAGRIETGTIILVERTLVYDIIE
                     QMGDGDEAIEFYASSLDLNWLPSNLPDSTRRGLLVSADRDIVVEDIPMLIKKYDAVAA
                     DWESGAIAWVAQRNKLRVLILRGVTDLVGGDGGEAYGNLGLFHERTKSVMGELFRQLP
                     DYLNLIKI"
     gene            complement(4840..6123)
                     /locus_tag="PLE39_13935"
     CDS             complement(4840..6123)
                     /locus_tag="PLE39_13935"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNE69481.1"
                     /translation="MKSITFQIIALCVMLSMILAACSPSVSLSVAESNKSRDKNPQIA
                     EGDLSTLVDGNNAFALDIYNALRSENGNLILSPYSMSLALAMTYAGARGDTESQMAQA
                     MHYQPQAQLHPAFNALDLALEKKPINLDKDQEPLQINIANSVWAEKTMPTLPDYLDTI
                     AVNYGAGIHLADFLNKPNEERVAINNWVSAETEDRIKDLLAEGSITPDTRMVLVNAIY
                     FKADWLHKFDANSSYDSTFHLLDGTEATVKMMGENIHDIPYMQGDGYQVVELPYAGET
                     AAMDIILPEEGNFEAFEASFNKDVYDEIVNGLQVVPSARVNLPKFEFTKDFSLSDALI
                     KLGMVNAFDRGLADFSGMTGEKDLFIGDVIHKAFVAVDEEGTEAAAATAVVMEATGAP
                     VQDIIFIADKPFLFIIRDTVNGQILFVGRVLNPAQ"
     gene            complement(6174..6641)
                     /locus_tag="PLE39_13940"
     CDS             complement(6174..6641)
                     /locus_tag="PLE39_13940"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69482.1"
                     /translation="MQTQHIKDAKMVITAVAQRIYMPEKTVQYFWDILEAEGELSDVD
                     DFQHAYHENHGLFLVVLDEEKVVGTGAVKKINADTAELKRLWLLEEYHGQRIGFQVVM
                     RLMEFARAQGYRRLRLQTSHKQIRAIHFYKRLGFYEIPSYRESMDNVSMEIML"
     gene            6723..7946
                     /locus_tag="PLE39_13945"
     CDS             6723..7946
                     /locus_tag="PLE39_13945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE69483.1"
                     /translation="MNTPEQKEIQKRNFRYVQIDAVGVSIANVAAPFLPVFLTRLGAS
                     NFQVGLLTSMPGITGLVLAILVGRFLQTRPNIVPWYSVSRLLVISCYALTGLLTFVLS
                     KEYAVISTLAIWAFATLPQTALAVAFSVVMNAVAGPEGRYALLSRRWAIFGITTVVMT
                     FFVTRVIDLVEFPRNYEMMFLGLSVGGFISYYFSKQIKLPDQTPPTPVPSSSVFDSAR
                     NYFSLIRGEPAFVSFAFKKFVYVSAITLSQPILPLFLVREVKATDSQIGTITMAMSLV
                     MLVGYFIWPWSSRKYGGRFVLLATTFGMILYPALTAATPQVPLIIIYAGITGLFQAGL
                     DLVFFDELMKTVPPEHGATFVSIVQSMQYLSAIAAPLIGTWLADFIGLGGALWVSAGL
                     RLLGFLLFLRKDTRR"
     gene            complement(7949..10045)
                     /locus_tag="PLE39_13950"
     CDS             complement(7949..10045)
                     /locus_tag="PLE39_13950"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE69484.1"
                     /translation="MPEWIGRTIGKVRVDKYLARGGMAEVYLGTHLSLERPVAIKVLH
                     SFIEEDPELLERFQREAKAVAGLRHSNIVQVFDFDTHDGHPYIVMEYLKGPSLASYLQ
                     TLHKNKQRLSFEQIGQLFKSIIAGMDYAHSQGIVHRDIKPANIILHSRNRDFEQDTPL
                     TKSVEAIITDFGLARMAHSGQQTASGFVSGTPTYMSPEQARGSKVDHRTDIYSLGIIL
                     YELLAGRVPFEADTAITIIFKHINEAPPPIEDIYPELQSVIDKALTKFPEDRYNSGHD
                     LLVDYYKAVGLHLEVETIHATRAQTPPKPATVIQKKKSTLNPLWVGTGIFLCACVSFF
                     LLSGVVGTGLFVLPRLNSTEAPTLPPTEIHTMATEAPVVNTETPVGILRFQDGAAIAD
                     QITISANLETLPEGSQYEAWMIEDSGEQRRSIGILSAEENGTFSLTYVDEQSRNLLSL
                     YSHMEITLEPIPDTSPNPSGDVKYSTGIPGRALAHIRHLLVSTEETPNQIGMMDGMRI
                     NAVLVNQTAEAMLLAFEENKQKDLRNNAEALINLIVGKQDPAYSDWNGDGTINDPGDG
                     YGILLNGEQTGYVGGVHHHSSYSADADDSTADIRMHNEHVEISIHNIEEWAIELRDLT
                     EKILQAPADANVEAEVRKAVALANQILNGLDVNGNEAIEPINGEGGALTAYQHAYYMA
                     DMPILIGKDQMPVTGK"
     gene            10302..10745
                     /locus_tag="PLE39_13955"
     CDS             10302..10745
                     /locus_tag="PLE39_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69485.1"
                     /translation="MSQNAQGTQSGGQETIIQRIRTSLTQADEYTRRQQRLNSTLTIS
                     TTVGSALTAFVTALTAAQGPTVIPGVLDWQGACTIGAIFSFITTVCSGLNQQMNTSQK
                     FLLGSQCVGRLRALELVATAQSRAMDEITNEYAGILREYAEALRK"
     gene            10842..11177
                     /locus_tag="PLE39_13960"
     CDS             10842..11177
                     /locus_tag="PLE39_13960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE69486.1"
                     /translation="MEITIQEFKHCDLFKVKGRVDSASAPEFSKALEKANNDGHFKIA
                     LDMSELEYMSSAGFRALLATQRNCKRYNRGELVLVSVPERIREALELAGFTELFKTFN
                     DSIEAVGSF"
     gene            11257..11667
                     /locus_tag="PLE39_13965"
     CDS             11257..11667
                     /locus_tag="PLE39_13965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE69487.1"
                     /translation="MPKITFPARFEYLDEIREFVGQVAREGGFNDKEVYSLQLASDEA
                     ASNIIEHAYEGVSNASFDVTCDVRGDTIIITMRDDGTPFDPTRVKQPNLKADLSERQI
                     GGLGVYLMRKLMDDVKYESNPRTGNLLIMTKRRG"
     gene            11675..14971
                     /locus_tag="PLE39_13970"
     CDS             11675..14971
                     /locus_tag="PLE39_13970"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE69488.1"
                     /translation="MRSSTRNGHPDSWDWRQLASLGEQLRNEDSLSAQRDRIASMTSD
                     LVAGVVDVWLNESVFRLPDWEDGSVFPEQPPKGGMRLAIKAGKITTQKTNRKQKKDAS
                     RKTQPPASRVTFAAVPLADHGITMGAFQVTRSKGPAFTTAELNMLQGIGQIVATSLFA
                     SHRAEVEQFRLRQLNLVREVSTQIANVLNVDELAARVTSLIQKTFNYYYVAIFTLNPN
                     SKFLRFRSSAVAPRKGKKKASIALEVEIGQGLIGESAKSGEQIVCDDVRSDARYRFID
                     SLPETQSEVVIPLKIEDRILGVLDVQSNQPHAFHPNDLLILHALADNIARAVEGARLY
                     GSLRRRADQLTLVSEVGRSVTSTLELSKLMRDAATLIHEKFKYPHVSLFTVHPNRRLI
                     VYEAGSGKRSHKLEGFTISLDDADGIMPWVARTGRTVLANDVSKHDRYLPSPLPPKNT
                     RSELCVPLIFNDDVLGLLDIQSDKLNAFTEDDQMMFEAVADTMAAAIRNADLYRSEQW
                     RRQVSDSLREVAGLVSNNVSVDEVLETILSELDRNLPIDVAAIWLLEDDDLCLSAVHG
                     CDPRLLESVCITNPDSTYAMAEALMSDAPIIRRADESLWPAGLALGYEQNYSSIAAPL
                     RVGDRSLGVLTLSHRTAGRYGHEAQAITTTFASYASVAIENARLFDAAQEQAYASAAL
                     LQVAQAVVSLNDLNEILASITRILPILVGVHRVSLYRWDSERESFITSHEFGFTEMEE
                     TTMTEKEFRLGDFPLLDFAREQNYMVAHPLDLGADPASWLQIQPEAEGEADLVNSERL
                     LMAVPLSIKHDLFGILLVEEAENGRRFRARRIEIINGIAQQAALAIQNDLLQQEMVVR
                     ERLETEVQLARQIQQTFIPHALPAHAAWQMAARWRTARQVGGDFYDVIELPNGKIGLF
                     IADVADKGMPAALFMALTRTLVRAAVIEMDSPAGVLGRVNDQLLPDTQQGMFVTAVYG
                     VLDVESGEFTYVNAGHNPPFWVNQNGNVEKLTRTAVALGVMEQPNMEERTVCLGFGDT
                     LLLYTDGLTEAFSPLGELFGDVRLMTALQAIQASTADEVLATVEEHLNEFIESTPLGD
                     DLTMLALRRV"
BASE COUNT         3318 a         3635 c         4053 g         3965 t
ORIGIN      
        1 tctgaacgag ccattgccag cacatccgaa tctttgtaat cggtggcaaa gatgatcttc
       61 tgccgttcga tgatggaact gatggctttt agctggtcgc gcacggcagc ggccttttcg
      121 aaacgcattt cttcagacgc cttgagcatt tcggcttcga gacgcttgat gatctcttca
      181 ctgtgcccgc tgaggaaatc catcaggtcg gagatcattt ggcggtatcc tgcctgatcc
      241 acggctccga tgcagggggc aaggcacagt ttgatgtcaa agtaaagaca cgcacgtttg
      301 tcgaggcctg taatttcacg gtcacacgtc aaatacggaa agatcttccg caatacatcc
      361 agtgtttgat acactgccca tgccgaggtg tagggaccaa aataacgtga accgtcttca
      421 tccatgttgc gggtgacggt taccctgggg aagggctcat gccagtggac tttgatatag
      481 gggtagcgct tgtcatcttt gaggcggatg ttgtatttgg ggcggtgctt cttgatgagg
      541 ttcatttcaa gaatcaacgc ctcaagctcc gacccgacca cgatccactc gatatccacg
      601 atgtcgcgca caaggcgtcg ggttttgtta tcgtggcttg aatcggcgtg gaagtaggac
      661 cgtacgcgat ttttcaggtt gatggctttg ccaacgtaaa tgatcttccc ttcaacattg
      721 cgatataaat aacagcctgg ctttgaggga agcgtggcaa gaatgccttg aagtttttct
      781 gaaatttcca ttgggcgcta ttatataata cccacggtca caaattgcgc tttccttctt
      841 ttgaaaatag ggcaatttgt gaccgagtgc ggtataaact caccttgcat tgagaggcgt
      901 atttttgaat agcatgaccc caatgtcctg tttgaaaaag atttcttttc tcttagaatt
      961 accgaaatca aacattaagg agaatgtaaa tggaaccgtc tttttcaaat gcccccattc
     1021 agaatggccc tactggtgcg agcggtggaa atgaaaagat catggcgatc gcctcgctgg
     1081 tgatcggtgt gatcaatttg tgcgcatggc ttttacccat ttgtggaatt ccactggctt
     1141 tggttggggt tgtcctcggt tttctgggta tgaaggatga gtcacaaaag atgcttgcga
     1201 tcgctggtat cgggctttct gcttttagcc tgttgctggc ttgcggcaat gccatctatg
     1261 gcgtttacgt cgctactcag ggtatgtctt tctaactctg ttgtataaaa agcagtcctc
     1321 cgccaaattg cggaggactg cttttttatt tatggactag gtgtgggggc ggggggcggc
     1381 actcggtgta tgccgagaag gagtggcatc tccgccatgt aatatgcgta ttcataggcg
     1441 gttgttgccc caccttcacc gacaatgggt tcgataagct ggttgttgtt cgaatcaacc
     1501 ccggagatga tctggtctga gagagtgagg atctcggtga tgatcggttc catttccggg
     1561 ccgaagggca tttcttgcag ccgaagcacc ttttccaaca cttgttcggt ccagccttgc
     1621 atgttttgca ggcagatgac tacatttgca ctttgcaatt ttatgttggc agttgaatcg
     1681 ggggcacggg cggcaaactg tgcatgtgac atgctctggg caatgtagcc ctgatcggta
     1741 tatccaggct cgccgttttg cagaaggcca aacccgtcac cactgtcact cacaacacca
     1801 tccccatccc agtctatgta ctggacggta ttggcgttgc cagcaatgag gttggcgatt
     1861 tcctccagct tttggcgggt gagggtttca tcgccagcct tgtaagcctc tcccaattcg
     1921 gtggttgtgg tgttcaggct ttctgtggct ccccacaagc cctgaattaa tgcaacacct
     1981 tcaggcgctg tgacgaatga gaccaccaca tgccgcacat ggatcaggct catgggggga
     2041 aagaccgagg atgccactac attcccagat gatgtgtctg ggttcgggtc gttatccact
     2101 tccagagtga tttcgatctg gtcgaacagt gaaaggacat tattttgttc agggttggtg
     2161 tacaccaatt gaccttgtgc aaggctttct ttggtgattg aacctgcctt acggacaaat
     2221 tccccgccct ggccgaggaa ccagacatcg taatgggtac cttcctcggg aatggggaga
     2281 tcatcgaatg aaagggcggc tttatccatc aacccgtttt gatcggtgaa gaccacacgg
     2341 ccgaggattt cattgggatc aacagttggt gttggcggag gaagagttgc ttcaggcgct
     2401 ggagtagcct gcggtgtttg cggtttgaag aagaccagtc cgagggctgt gatcgcaacc
     2461 acaccgagtg caacgccgac ccacatgagg gttgggttgt tgcgcgttga cttctttcct
     2521 tctgcggcgg cttcggcagc gggtttgaca accctctgtg gaattttagc aatacgggcg
     2581 gtgtcggcag aaatggtctg tccgttgaac acagccagaa attcatcggc cattgccttg
     2641 gcagagtcat agcgcaattc ggggtctttt gaaagtgcgc ggttgataat ggcttgcagg
     2701 tctgatgaaa tgccgaagat gggcggcggc gggacattga gatgtttcat gagaatgccg
     2761 aaggatgatt cggcatcaaa gggcaccaca cccgccagca tttcatacaa catcacacca
     2821 agcgagtaga tatctgtttt ggagttgact ttttcgccgc gggcctgttc agggctcatg
     2881 taggcgggcg ttccagagac cgttcctgtt gaggtttgga tggatgaatc tagaaggcgg
     2941 accagcccaa agtcggtgag tatcggttcc acatccgcgg gtaagggttt gttgaggtca
     3001 atggggctgc ttggtgagcg caagagcaca ttggcgggtt tgatatcccg atgaatgatg
     3061 ttttggttgt gggcgtaatc aacggcagaa gatagtttgg aaaggatgag tgccacggtt
     3121 tcgagcggaa gtttttctcc gcgtttgtgc agggctttca agtaattccc catggatgcg
     3181 cccgtcacca gttccatgat gaggcatggt tggccatcta caagttcata atcgtacact
     3241 tgaatgatgt ttgggtgacg aagccccgcc accacccgcg cttcacgttc gaaccgcacg
     3301 tggttatcag gatcagagtc aacatggtcg cgcatgatct tgacagccac ttttcgatta
     3361 agtgtggtgt gctcgccaag ataaacttcc gccatgcccc cgcgggcgat caattctccg
     3421 acttgtacct tacctaaagt ttttccattc caacttggca ttgacgcatt atacccgata
     3481 gtataatgcg aaggattaca aggagatttt aatgacacat gcacttgaag aaaccctgcg
     3541 cgaacatttg cgcacacaag gtttaacagt agtcaccgcc gagtcctgca cgggcgggtt
     3601 ggtttcagat cgtattacca atatctcggg ttcgtcggag tattttcctg gcgggatcgt
     3661 ggcttattcc tatgaagcca aagccagcct gctcggtgtt tcgtgggata cgctcaatac
     3721 gcatggtgcg gtcagtgagg aaaccgtgct cgaaatggcg cgtggtgtgc ggaaattgtt
     3781 caagtcaaac atcggaattt cggtcagcgg aattgctggg ccaacgggcg gcacacccga
     3841 aaagccagtg gggttgacat ggttcggcct ttccactgac aaaggagaat gggcgcgtca
     3901 ctttatttgg gatggcgacc gcctgcaaaa caaacaccat tcctctgaag ccgctttgca
     3961 atttgtgctc gattatctgg atggaaaact ggaatgaagg cggttgtcat tgtttcagcc
     4021 aatgccgaat ggcgcgtggt aaaggaactg tacccgaacc tcgaaataaa aaactcgcct
     4081 tacggcgaat acgcccacct gacacttgga acctggaacc tggaactctt ccacggtggt
     4141 tggggcaaga tctctgccgc agcaacagca caatatgtaa ttgatcattt ccagcccgat
     4201 ctgctgatca acctcggtac ctgtggcgga tttgcaggcc gcattgaaac ggggacgatc
     4261 atcctcgtag agcgcaccct ggtctatgac attattgagc agatgggaga tggcgacgag
     4321 gcgattgaat tctacgcatc ttctctggac ctgaactggc ttccctctaa tttacctgat
     4381 tccacccgcc gcggactcct cgtctctgcc gaccgtgata ttgtggtgga agatattccc
     4441 atgctcatca aaaaatatga tgcagttgcc gccgattggg aatcaggggc aattgcctgg
     4501 gttgcacagc ggaataagct ccgcgttttg attttgcgcg gtgtgacaga tctggtcggc
     4561 ggtgatggcg gcgaagccta tggaaatctc gggttgttcc acgagcggac aaagagcgtg
     4621 atgggagagt tgttccgtca attgcctgat tatctaaatt taataaaaat ttgaggtgta
     4681 gcgcgagata ctatctcgcg ctacagacat tgtcatgcat ttcctcccat caccgaagta
     4741 ggctttgttg cagcgttgtt tccatgcagg cttgaaatca acacatcccc cgcatgcaag
     4801 cgcgggggat gtgttttcag atgcggataa tttgagtttt tactgggcag ggttcagcac
     4861 acggcctaca aataaaatct gtccgttgac cgtatcgcgg atgataaaaa ggaagggctt
     4921 gtcggcgatg aagatgatgt cttgcactgg cgcgcccgtc gcttccatta ccacggctgt
     4981 agcggcggcg gcttcggtgc cttcctcgtc cactgctaca aaggccttgt gaatgacatc
     5041 gccgatgaac aggtcttttt cgcctgtcat tcctgagaaa tcagccaaac cacggtcaaa
     5101 ggcgttgacc atgcccagtt tgatcagggc atcggaaagg ctgaaatctt tggtgaattc
     5161 aaacttgggc aggtttaccc gcgccgaagg aacaacctgt agaccattga cgatctcatc
     5221 gtaaacatcc ttattgaagg atgcttcaaa agcttcgaaa tttccttcct cgggcaggat
     5281 gatatccatg gcggcggttt cacctgcata gggaagttca acaacctgat acccatctcc
     5341 ctgcatgtag gggatgtcgt gtatgttttc gcccatcatt tttactgtgg cttctgttcc
     5401 atccaaaagg tggaatgtgc tgtcgtatga tgagttggcg tcgaatttgt gcaaccagtc
     5461 agccttgaag taaatggcat tgacgagtac catgcgggta tcgggggtga tgcttccttc
     5521 ggcgagcagg tctttgattc tgtcttcggt ttcagcgctg acccaattgt tgatcgccac
     5581 acgctcttcg ttgggcttgt tcaaaaaatc agcgaggtgg atgcccgccc cataattgac
     5641 ggcaatggta tcgaggtaat cgggcagagt gggcatggtt ttctcggccc aaaccgaatt
     5701 ggcgatgttg atctgtaaag gttcctgatc tttatccagg ttgatgggct ttttctccag
     5761 tgcaagatcg agagcgttga aggcgggatg gagttgggcc tgcggctggt agtgcatggc
     5821 ctgtgccatt tgcgattctg tatcgcctcg ggcgccagcg tacgtcatgg ccagggccaa
     5881 agacatgctg tacggagaga ggatcagatt tccgttttcg gagcgaagcg cgttgtaaat
     5941 atccagcgca aaggcgttgt tcccatccac aagggtagac aggtcacctt cggcgatctg
     6001 cggatttttg tcgcggcttt tgtttgattc agccacactt aatgataccg atgggctaca
     6061 ggcggccagt atcatgctca gcatgacaca taatgcgatg atttgaaaag tgattgattt
     6121 catggtttct ccttcgaact aaagacgaat tcgttttaat tatgttcctg ctgttacagc
     6181 ataatttcca tggaaacgtt gtccatgctt tcgcgatagc ttggaatttc gtaaaacccg
     6241 agccgtttgt aaaaatgaat ggcgcggatc tgtttatggc tggtttgcag gcgaaggcgg
     6301 cgatagccct gtgcgcgggc aaactccatc agccgcatga ccacttgaaa cccgatgcgc
     6361 tggccgtgat attcctccaa aagccagagt cttttgagtt cagccgtgtc tgcgtttatt
     6421 tttttcactg cacccgtacc gaccactttt tcttcgtcga gcaccaccaa aaacaagccg
     6481 tggttttcgt gataggcatg ttgaaaatca tccacatcac ttaattcacc ctccgcttcg
     6541 agaatatccc aaaaatactg cacggttttt tcaggcatgt aaattcgctg agccacggcg
     6601 gtaatgacca ttttggcatc tttgatatgt tgtgtttgca tgaggtcgat acgaatcatg
     6661 tcgtgattct aacccgcttc gtggagcgtt ataatcccag aacgtcaccc atacctccta
     6721 tgatgaacac tccagaacaa aaagaaattc aaaaaagaaa ttttcgttac gtgcaaattg
     6781 atgcggtggg tgtaagcatt gccaatgtcg ccgcgccttt cctgccagtt tttctcaccc
     6841 gcctcggtgc atctaatttt caggtaggcc tgctcacctc catgccgggt atcaccgggt
     6901 tggtgctggc cattcttgtc gggcggtttt tgcaaacccg tcccaatatt gtgccatggt
     6961 acagcgtttc gcgtttgctg gtcatttcct gttatgcgct gacaggcctg ctgaccttcg
     7021 tgctttcgaa agaatacgcg gtcatatcca cgcttgccat ttgggccttt gccacacttc
     7081 cacagactgc gttggcagtg gcattctcag ttgtgatgaa cgccgtggcg ggtcctgaag
     7141 gccgttatgc cctgctcagc cgccgttggg cgattttcgg aatcaccacc gtggtaatga
     7201 cctttttcgt cacccgtgtc attgatctgg tggaatttcc gcgcaactat gaaatgatgt
     7261 tcctggggtt atcagtgggc ggattcatca gctattactt ttcgaagcag atcaaactgc
     7321 ccgatcagac tccgccgacg cctgttccgt cctcatcggt atttgattca gcccgaaatt
     7381 atttctcctt aattcgcggc gaaccagcct ttgtatcctt cgcattcaag aagtttgtgt
     7441 acgtttctgc catcactttg agccagccca ttctgccgtt gtttttggtg cgcgaagtga
     7501 aagccactga ttcacagatc ggtaccatta caatggcgat gtcgctggtg atgctggttg
     7561 ggtacttcat ctggccctgg tcttcgcgca aatatggcgg acggtttgtc ctgcttgcca
     7621 ccactttcgg catgattctc taccccgcac tcactgctgc caccccgcag gtcccgctga
     7681 ttattattta tgcggggatc accgggctat ttcaggcagg tcttgatctt gtattctttg
     7741 atgagttgat gaaaaccgtt cccccggagc atggcgccac ctttgtttcg attgtgcagt
     7801 ccatgcaata tttatctgcc attgccgcac cgttgatcgg cacatggctg gcggatttca
     7861 ttggcctggg cggcgcgttg tgggtgagtg ccgggttgag gttgttgggc tttttgcttt
     7921 ttttgcgcaa agatacgcgc agataacttt acttccccgt gacaggcatt tggtctttgc
     7981 cgatcaaaat gggcatatcg gccatgtagt aggcatgttg atatgctgtg agcgctccac
     8041 cttcaccgtt gatgggctca atggcttcgt tgccgtttac atccaacccg ttgaggattt
     8101 ggttggcaag cgccacagcc ttgcggacct ctgcttcgac attggcatct gcaggggctt
     8161 gtaatatctt ttcagtcagg tcgcgcagtt caatggccca ttcttcaatg ttgtgaatgg
     8221 aaatctccac atgctcgtta tgcatacgga tatccgctgt agagtcatcg gcatcggcgg
     8281 aatagctgga gtgatgatgc actccgccta cataaccggt ctgttctcca ttcaagagaa
     8341 tgccataacc atcaccgggg tcgttgatcg ttccatcacc gttccagtct gaataggcgg
     8401 ggtcctgctt cccgacgatc aggttaatga gcgcttctgc attgttacgc aggtcttttt
     8461 gtttgttttc ttcgaaggca agcaacatgg cttctgcggt ctgattgacc aaaacggcat
     8521 tgatacgcat tccgtccatc ataccgatct ggttgggtgt ttcttccgtg ctgacaagca
     8581 ggtgacggat gtgcgccaat gcacggcccg gtatgccggt ggagtatttc acatctcctg
     8641 atgggttggg gctggtatcg gggatgggtt ccagcgtgat ttccatgtgg ctgtacaggc
     8701 tgagcaggtt gcggctttgt tcatccacgt aggtgaggct gaaggttcca ttctcttcgg
     8761 cgctgaggat gccaatactg cgccgttgtt ctccgctgtc ttcgatcatc catgcttcgt
     8821 attggctgcc ttctggcagg gtttcgagat tggcactgat ggtgatctgg tctgcaatgg
     8881 ctgctccatc ttgaaagcgc agaatgccaa ccggggtttc ggtgttgacg acgggggctt
     8941 cggtggccat ggtgtggatt tctgttgggg gaagggtggg cgcttctgtg gagtttaatc
     9001 tcggcagcac aaaaaggcct gtgccaacga caccgcttag caaaaagaag gaaacgcagg
     9061 cacaaaggaa gattcctgtg ccgacccaaa gtggattcag agtggatttt ttcttttgaa
     9121 taacagtagc aggtttgggc ggggtttgag cgcgagtcgc gtggatggtt tccacctcga
     9181 gatgtaatcc aactgctttg taataatcca ccaacaggtc gtgtccgctg ttatagcggt
     9241 cttcggggaa tttggtgagg gccttgtcaa tgacagattg cagctcaggg taaatatctt
     9301 cgatgggcgg gggcgcctcg ttgatgtgtt tgaagatgat cgtgattgcc gtatcggctt
     9361 caaaaggaac gcggcctgca agcagttcat acaggataat gccgagtgaa taaatatcgg
     9421 tgcggtgatc cacctttgat ccgcgggctt gttccgggct catgtaggtg ggcgtaccgc
     9481 tgacgaatcc tgatgcggtt tgttggcctg agtgtgccat gcgggccagg ccaaaatcgg
     9541 tgatgatggc ttccacactt ttggtaagcg gggtgtcctg ctcaaaatcc ctgtttctgc
     9601 tgtggaggat gatgttggcg ggtttgatat cgcgatgaac gatgccctga gaatgggcat
     9661 agtccatgcc tgcaatgatg gatttgaaca actgaccgat ctgctcaaag gataatcttt
     9721 gtttattttt gtgaagggtt tggagatagg aagcaagcga tgggcctttg aggtattcca
     9781 tcacgatata gggatgcccg tcatgtgtgt cgaaatcaaa tacctggaca atgttggaat
     9841 gacgcaggcc tgccactgct tttgcttcac gttgaaaccg ttccaaaagt tcagggtctt
     9901 cttctatgaa gctgtgtaga actttgatgg ctacgggacg ttcaagtgac aggtgggtac
     9961 caagataaac ctccgccatg cccccccgag cgagatattt atctacccgg acttttccga
    10021 tggtacggcc gatccattca ggcatgagtt gattatacat ctgaatttgg agtgttgtag
    10081 tctgaaatgc aggtgaaatg atcgttgatg gaggtgtctg ggtgttactc aaagtttgct
    10141 gtaatctggc tcaaagccgc cgtcctcggc gaagggagta aatttttaac acaccccaag
    10201 tgcctgttcg aaaaattttg gagggggttt tgccttataa ttcgacgctt acaggtaaaa
    10261 gaattttttc aaactttttt atgaacaagg atggaggaaa catgtctcaa aatgcgcaag
    10321 gcacccagtc tggcgggcag gagaccatca ttcaaaggat tcgtaccagc ttgacacagg
    10381 cggatgaata cactcgccgt caacagcgcc ttaactccac attgaccata tccactacgg
    10441 ttgggtctgc gttgacagct tttgtcactg cgttgactgc cgctcaaggt ccaacggtca
    10501 ttccaggtgt tttagattgg cagggcgctt gcaccattgg cgcgatcttc agttttatta
    10561 cgacggtctg ctccgggttg aatcaacaga tgaacaccag ccaaaaattc ctgcttggaa
    10621 gtcaatgtgt ggggcggtta cgggccttgg aactggttgc aacagcccaa tcacgcgcca
    10681 tggatgaaat tacaaatgag tatgcaggga tcctgcggga atatgccgaa gcacttcgga
    10741 aataggccgc gatttttata aacaccctgt aatgccccat aatagaacgt gttataattt
    10801 tcaaccgcaa ctcgaaaatc cgtcttgaaa gaggagtata tatggaaatt acaatccagg
    10861 aatttaaaca ttgtgatttg tttaaagtaa agggcagggt tgatagtgca tctgcaccgg
    10921 aattttccaa agctctcgaa aaagccaata atgatggcca tttcaaaatt gctcttgata
    10981 tgagcgaact tgaatatatg tccagcgccg ggttccgcgc tttgctcgcc acccagagaa
    11041 attgcaaacg ttataatcgc ggagaactgg ttctggtaag tgttcccgag cgcattcgtg
    11101 aagccttgga attggctggt tttaccgaac tcttcaagac cttcaatgac tccattgaag
    11161 cggttggcag cttctaatcg acgcctgggg cgtcgcaatt aaaaataatc gccgcctctt
    11221 tgccgaaagg acggaggcgg ctattaattt ttacccatgc caaagatcac ttttccagcc
    11281 aggttcgagt acttggatga aatccgcgag tttgtagggc aggttgcccg cgaaggcggt
    11341 ttcaacgaca aggaagttta ctccttgcag ttggcttcgg atgaagccgc ttccaatatc
    11401 attgaacatg catatgaagg cgtatcaaac gccagtttcg atgtcacctg tgacgtgcgt
    11461 ggcgatacga tcatcatcac catgcgtgat gatggcacgc cgtttgaccc cacccgggtg
    11521 aagcagccca acctgaaagc ggatctgtct gagcgtcaga tcggcgggtt gggtgtatat
    11581 ctcatgcgga aactcatgga tgacgtgaaa tatgaatcca atccgcgcac gggcaattta
    11641 ttgatcatga ccaaacggag gggataacct cctcatgcgt tcgtcaactc gtaacgggca
    11701 tccagactcc tgggattggc gtcagcttgc aagtttgggg gaacaacttc gcaacgaaga
    11761 ctccctcagc gcacaacgtg accgcatcgc ctccatgacc agtgaccttg ttgctggggt
    11821 ggtggatgtc tggttaaacg agagcgtatt tcgcctgcca gattgggagg atggaagtgt
    11881 atttcctgag cagccaccca aaggcgggat gcggcttgcg atcaaagcag ggaagatcac
    11941 cacccaaaag accaatcgaa agcaaaaaaa agatgcatct cgcaaaacac agccgcccgc
    12001 ttcccgcgtg acctttgcgg ctgtcccgct tgcggatcat ggtatcacca tgggggcttt
    12061 tcaagtcacc cgttccaaag ggcctgcctt caccaccgct gaattgaata tgcttcaggg
    12121 tatcggtcag atcgtggcga ccagcctgtt cgcttcccat cgtgccgagg tggagcagtt
    12181 tcgcctcagg cagcttaatc tcgtccgcga ggtcagcacg cagatcgcga atgtgctgaa
    12241 tgtggatgaa cttgcggcac gcgtcaccag cctgattcaa aagacattca attattacta
    12301 tgttgccatt ttcaccctga acccgaattc gaaattcctt cgttttcgtt ccagtgcggt
    12361 tgcgccgcgc aagggaaaaa agaaggcttc cattgctctt gaagtcgaaa tcggccaggg
    12421 gttgatcggc gaatccgcca aaagtggtga acagatcgtt tgtgatgacg ttcgttctga
    12481 tgcgcgttac cgcttcattg acagcctgcc tgagacacaa tctgaagtgg tcattccgct
    12541 caagatcgaa gaccgaatcc tcggcgtgct ggatgtgcaa agtaatcagc cgcatgcctt
    12601 ccatcccaat gacctgctga tcttgcatgc actggctgat aacattgccc gtgctgtgga
    12661 aggcgcccgt ttgtacggca gcctccgccg ccgtgccgac cagttgaccc ttgtctctga
    12721 agtagggcgg agtgtcactt ccacgcttga actatctaaa ctcatgcgtg atgccgccac
    12781 tctgatccac gaaaaattca aataccccca tgtttccctg tttaccgtcc atcccaaccg
    12841 ccgcttgatc gtgtacgaag cagggagcgg taaacgcagc cacaaactgg aaggctttac
    12901 catttcattg gatgatgccg atggcatcat gccctgggtg gcgcggactg gccgcacggt
    12961 tcttgcaaat gatgtttcca agcatgaccg ttacctgcct tcacctttgc cgccaaagaa
    13021 caccaggtct gaactttgtg ttccgctgat cttcaacgac gatgtgctcg gcttgctgga
    13081 tattcaatcc gataaactga atgcctttac ggaagacgat caaatgatgt ttgaagccgt
    13141 ggccgatacc atggctgccg ccattcgcaa tgcagatttg tatcgctccg aacaatggcg
    13201 gcgtcaggtt tctgacagtc tgcgcgaagt ggcgggtttg gtctccaaca atgtaagcgt
    13261 ggatgaagtg ttggaaacca ttctctctga actggaccgc aacctgccca ttgatgtcgc
    13321 tgccatttgg cttttggaag atgatgacct gtgcctttcg gctgtgcatg gttgtgaccc
    13381 gcgcctgttg gaaagcgttt gcatcaccaa tcccgattcc acttatgcca tggctgaagc
    13441 cctgatgtct gatgcgccca tcattcgccg tgctgatgag tcgttgtggc ctgctggcct
    13501 ggcattgggc tacgagcaga actattcgtc cattgcggct ccgcttcggg tgggtgatcg
    13561 gtctttgggc gtcttgaccc tgtcacatcg cactgcgggt cgttacgggc atgaagcaca
    13621 ggccatcaca accacgttcg caagttatgc ctcggttgcc attgaaaatg cccgcctgtt
    13681 cgatgccgcc caggaacaag cctacgcatc ggcggccttg ttgcaagtgg cgcaggcggt
    13741 cgtcagtttg aacgacctca acgagattct cgcgtccatc acccgcatcc tgccaatttt
    13801 ggtgggcgtg catcgtgttt cgctgtatcg ctgggattca gagcgggagt cgttcatcac
    13861 ctcgcatgaa ttcggtttca ctgaaatgga agaaacgacc atgaccgaga aggaattcag
    13921 actcggcgat tttccattgc tcgattttgc ccgtgagcaa aattacatgg tcgcgcatcc
    13981 tctcgacctg ggtgctgatc ctgcctcctg gttacagatt caacccgagg cggagggcga
    14041 agcggatctt gtcaactctg agcggctgtt gatggccgtc cctttatcca tcaagcatga
    14101 cctgttcggc attttgctcg ttgaagaagc cgaaaatgga cgccgcttcc gcgcgcggcg
    14161 tattgagatc atcaatggca ttgcacaaca ggcggcgctt gccattcaaa acgacctgtt
    14221 gcaacaggag atggtggtac gcgaacgcct cgaaaccgag gtgcagcttg cccgccagat
    14281 ccaacagacg tttattccgc atgcactgcc tgcacatgca gcctggcaaa tggcggcacg
    14341 ttggcgcacg gcgcggcagg tgggcggtga cttttacgat gtgatcgaat tgccaaacgg
    14401 aaagatcggc cttttcatcg cggacgtggc cgataaaggg atgcccgccg ctttgttcat
    14461 ggcgctcacc cgcaccctgg tccgtgcggc tgtgatcgag atggattcgc ctgccggtgt
    14521 gctgggacgt gtgaacgacc aacttttgcc cgatacccag cagggcatgt ttgtaacggc
    14581 tgtgtatggc gtgttggatg tggaaagcgg tgagtttact tatgtgaatg cgggacacaa
    14641 cccgccattt tgggtgaatc agaatgggaa tgtggaaaaa ctcacgcgca cagcggttgc
    14701 gttgggtgtg atggaacagc ccaatatgga ggaacgtacg gtttgccttg gttttggtga
    14761 taccttgctg ctctatacag atggacttac cgaagcattc tcaccactgg gtgagttgtt
    14821 tggcgatgtg cgtttgatga cggcgctgca ggccattcaa gccagcactg cggatgaagt
    14881 ccttgccacg gtggaggaac acctgaatga attcatcgag tccacgcctt tgggcgatga
    14941 tctgaccatg ctggcgttgc gtcgggtgta a
//
LOCUS       DAOQJC010000099        14960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1903_length_14960_cov_9.488963, whole genome shotgun sequence.
ACCESSION   DAOQJC010000099 DAOQJC010000000
VERSION     DAOQJC010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14960
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1903_length_14960_cov_9.488963"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2320)
                     /locus_tag="PLE39_13975"
     CDS             complement(<1..2320)
                     /locus_tag="PLE39_13975"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE69489.1"
                     /translation="MAKVSLRIYNREIEGLIDQAHINEAIAHCHHILRTFPKHLATYR
                     LLGKAYLELKRYGEAVDIFSRVLMSVPDDFVSHVGMSIIRDEENKLDDAIWHMQRAFE
                     AQPSNSAIQGELQRLYGRRDGMEPPKIRMTRGALAHMYVQGELYPQAIAEIRAVLAQE
                     PDRADMQVLLARAYFHGGQKADASDMCMQLLKRYLYCYDANCIMVDLLPATAGAVEST
                     QVYRMRVGELDPYTAFAKGSVFLANEVPDASINLERLDYKEEDAPATQSWGASSSSLG
                     LGSGASSAPMLSSSNSEPDWLKNASDTSASKSGEDIPDFLRAAGWGESTGPEQPTSFF
                     DDTPASGDLTPADIPDWLKGQVPTNVSQPAAPQPASSQSIETPDWLSGLDTGSETSSA
                     SGDIPDWLSGLGTAGAVAAASSAQSEPAPAQPGNVPDWLSGLGDAATPSEPASSIPPA
                     QPGNVPDWLSGLGDDATPSEPVSSIPPAQPGNVPDWLSGLGDEPTTTNAAQTDDVSDW
                     LSGLDSPAPVAAPTPATPSQSSDVPEWLSGLGDAEAAKSAQPDDVMEWLNGLDTPKTS
                     VAPAAAASGDVPDWLRDIDDSQTPSGNGPDSLFADVQAAPAQSDDLPAWMSGLGETSQ
                     PAASQPTSDSDLPAWMTGIPDAQPDELGQTGEATEWLHSISTDAPLSQTPAPSVESLG
                     STAQEQDDAVAWLESLAAKHGAKPEELVTDPNKRSETPPEWVSQAQNIEQQTPAAQTP
                     APSVESLGSTAQEQDDAVAWLESLAAKHGAKPE"
     gene            complement(2385..3680)
                     /locus_tag="PLE39_13980"
     CDS             complement(2385..3680)
                     /locus_tag="PLE39_13980"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNE69490.1"
                     /translation="MRAVDIIIKKRDKQELTSQEIDFFIQGFASGEIPDYQASAFAMA
                     VLLNGMTPFETADLTLAMAKSGQVLDLSKVVDIAVDKHSSGGVGDKTSISVMPMVAAC
                     GLPVGKMSGRGLGFSGGTLDKLEAIPGYRVNLTTDEFKQQLKEKGIVLTGQSLDLAPA
                     DGKMYALRDVTGTVPSMPLIASSIMSKKIAAGAQAIVLDVKTGLGAFMQTLEDAHTLA
                     DLMVDIGRLAGRKTVALISDMNQPLGSAVGNALETIEAIELLRGHAPFDYYEHCLHVA
                     AHVLVLGQRAKDLDEGRVMAEKSITDGSALEKFRVLVQAQGGDVSYVDDISKFEQAKY
                     VEVVNAPRSGFISQVHARLVGEASVLLGAGRSKKTDPIDHAVGFLIYKKVGDQVQAGE
                     PLFAIHANDETKLAEARKAVLAAYTFSDEFVSPLPLFYG"
     gene            complement(3839..4867)
                     /locus_tag="PLE39_13985"
     CDS             complement(3839..4867)
                     /locus_tag="PLE39_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69491.1"
                     /translation="MTLGRIAFLGSGETSLAGGRIFETLARLIPDPLRVAILETPAGF
                     ELNASLVANRVADFLKTRLQNYKPTIDLIPARKQGTEFSPDNPDILKPLLQANLIFMG
                     PGSPTYTARQLRGTLAWDIIRARHRQGATLVFASAATISVGAWALPVYEIYKVGEDVH
                     AKEGLNLFSDFGMNVSFVPHWNNAEGGIDLDTSRCFVGLERFDQWHRLLPPENIIVGL
                     DEHSGVIMDCEQGTCDVHGVSSVTVMKNKDMEMYPTGASFSLSELGNCALPVPLEKGI
                     RPEIWEMLNKASSEEGPSEEVLKLLELRQQARVAKNFAESDRLRDQIASQGWVVQDSK
                     EGQKLIKG"
     gene            complement(4869..5327)
                     /locus_tag="PLE39_13990"
                     /pseudo
     CDS             complement(4869..5327)
                     /locus_tag="PLE39_13990"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(5387..6349)
                     /locus_tag="PLE39_13995"
     CDS             complement(5387..6349)
                     /locus_tag="PLE39_13995"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE69492.1"
                     /translation="MKRWKQILFRIFLFACLVVLFGPFLVPVPELQGLVTEAELADPD
                     SKFIEVNDVTVHYKEKGEGNTTFILLHGFGSSVFSWREVMDDFSKYGRVIAYDRPAFG
                     LTERPMPETWQENPYGMGANVELLRGLMDTLKIEKAVLVGNSAGGGVSVAFALTYPER
                     VDKLILVDPGVGGGYGPQFPAWALPVMWTPQMRHLGPLMMRDYQETLPNTITREWANE
                     NNLSAEVRQEYLNALQIKNWDRAFYELTFAPAYPELRPLLPNLTVDTFIIAGADDRLI
                     RTWYFEAISQETPAPLVLLPQCGHVPHEECPEQFMQEVLKFLEL"
     gene            complement(6386..7120)
                     /locus_tag="PLE39_14000"
     CDS             complement(6386..7120)
                     /locus_tag="PLE39_14000"
                     /inference="COORDINATES: protein motif:HMM:NF024107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE69493.1"
                     /translation="MKIIKRVLFGLVLVVGLAVLGFVIWAETPLGPAPEALAALESDS
                     NVTVTIGDAITFEPVNIKPVTGFIFYPGGHVDYRSYAVPLRAIAAQGYLVVLLPVKLN
                     LAFFDVNAGDPVFAVHPEIQQWVVGGHSLGGVASALFAKDHLDTVDGIVFWASYPADD
                     SLKNSSLKMLSIYGTNDMAGMEQFSETSALLPASTEYVVIEGGNHAQFGDYGPQPGDK
                     QATISRTEQQTQVVNAVVKFLEELVH"
     gene            complement(7107..7793)
                     /locus_tag="PLE39_14005"
     CDS             complement(7107..7793)
                     /locus_tag="PLE39_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNE69494.1"
                     /translation="MKTNLETLNEEIISCRKCPRLVEWREEVARVKRKAYKDDEYWGR
                     PVPGFGDPKARVLVVGLAPGAHGSNRTGRQFTGDASGGFLFPALYRAGFANQARAESR
                     SDGLILKDMYITASGRCAPPENKPNPEELNTCQPFLERELQLIQPSVIVCLGKIAFDR
                     ILKITATRGLKFAHGAVHTLLPSSFINHPSSLICSYHPSQQNTLTGKLTVKMFDEIWE
                     KAKLLLNENH"
     assembly_gap    7814..7913
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8027..9079)
                     /gene="queA"
                     /locus_tag="PLE39_14010"
     CDS             complement(8027..9079)
                     /gene="queA"
                     /locus_tag="PLE39_14010"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNE69495.1"
                     /translation="MKTSDFDYVLPESSIAQTPADPRDSSRLLVLHRDSGDVEHRVFR
                     DLSLFLQPKDLLVLNRTRVIPARIFAKKETGGKVELLLLRRRDTVTWEALVGGKGLRV
                     GKKVVVEVEEAGRADNFLQAEIIEILEGSERLIRFSEAIEPYFPKVGNIPLPPYIHEK
                     LSDPERYQTVFAKEAGSAAAPTAGLHFTPQLLDTLQNKGVKVGYVTLHVGLDTFAPVT
                     EESPEEHKIHTEWCELPQETADLINQTRQNGGRVVAVGTTSVRTLESAASRSVIGNRT
                     SDIGAFTGPTSIYILPGYQFKVVDAMVTNFHLPKSTLIMLVSAFAGRERILDTYNLAV
                     REGYRFYSFGDAMFID"
     gene            complement(9100..9963)
                     /locus_tag="PLE39_14015"
     CDS             complement(9100..9963)
                     /locus_tag="PLE39_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69496.1"
                     /translation="MKISLRLLPLFFLLSACGRLDSILYTPQQTPETWLQIQPFSEFQ
                     IASQKVVFVQPSTSIIVYFLGLLTIGVGLYFLKLRDGQRSRFWWGVALLLWGIGALLA
                     GTSYEAFSYAIKCEGRTACLWTSWWEIAYLIVSVWSIDAMMLAVAFTSTDGKLRKILS
                     VYSIVNAVFYFAVVMAGAFIPVKFLISFELLLIVAAPSVIAFFVINGWRYAKHKLKSD
                     LVALGAWLWLGLTIAAYFLYLISGNTTVLWEKGFWFSENDVLHIGLIIWMIYLALVLA
                     PHVRDMNQEIS"
     gene            10065..11459
                     /locus_tag="PLE39_14020"
     CDS             10065..11459
                     /locus_tag="PLE39_14020"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE69497.1"
                     /translation="MKLSFHGAAQTVTGSMHLVEINGSRLLLDCGLYQGKRKESIEKN
                     RNFPFDPKTIDAVILSHAHIDHSGNLPNLIKQGFSGLIYCTSATAELTDIMVRDSGHV
                     QEKQAESTSYHNAKRGEPPVDPLYTEEDAKEVEPLLQPVRYDVPFEPANGVIARLQDA
                     GHILGSASISLHLEEKRIRKHLWFSGDIGRRNMPILRDPVLPKDDVDVLMMETTYGDK
                     PHRDPEEAYNEMRLVIKRTLDRGGKVIIPSFAVGRTQELVYNLSRMYDEGDLPNVPVY
                     VDSPLAVNASKIFRKHEECYDAEMKALVHSGHPALDFKLLTYINSLEESKELNARTDP
                     MIIISASGMAEAGRIVYHIRWGIENKKNTIMIVSWQSPETLGRKLAEREPNVKIFGET
                     YRCRAEIVTIGGLSAHAGQDMLLEYASGVRQKAEYIFLVHGEDKGAIPLREKLREKNI
                     KQIYYPAMHTSVEL"
     gene            complement(11466..>11678)
                     /locus_tag="PLE39_14025"
     CDS             complement(11466..>11678)
                     /locus_tag="PLE39_14025"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HNE69498.1"
                     /translation="RYLMLGGIILFLIGGGVYLAARFGIPLGRLPGDIRIEGENGSFY
                     FPVTSSILISVILSLLLNLISKLMNK"
     assembly_gap    11679..11778
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11852..12238)
                     /locus_tag="PLE39_14030"
     CDS             complement(11852..12238)
                     /locus_tag="PLE39_14030"
                     /inference="COORDINATES: protein motif:HMM:NF039803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HNE69499.1"
                     /translation="MIEQYFQSMLDLLAALPFVESSSVNLEKRGDLAGFIRGEVEFRD
                     GSSLLFFRELIDLRPPLQKIMYAYHYQNADGDLIFRYDNTAHHRSVPTFPVHKHTMGG
                     GVTSSEVPSLEQVLREIEGFILQGRV"
     gene            complement(12231..12521)
                     /locus_tag="PLE39_14035"
     CDS             complement(12231..12521)
                     /locus_tag="PLE39_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69500.1"
                     /translation="MLQEVIIKTDSNEALKPLLEVAIQNQLKSLLHGIQRTKERVAEF
                     EKRAGMSSAELEKKLQSNEVEETVEIIDWNMELAALRLLEGQYQSLSEAKID"
     gene            complement(12554..13597)
                     /gene="ruvB"
                     /locus_tag="PLE39_14040"
     CDS             complement(12554..13597)
                     /gene="ruvB"
                     /locus_tag="PLE39_14040"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012914214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNE69501.1"
                     /translation="MSKSPSSSKNSGRALDPESKPDDRVDNALRPQKLDDLIGQDQVK
                     ENLSILIDAARARGEALDHVLFYGPPGLGKTTLAHVLGNEMGVNVKVTAGPAIERAGD
                     LAAILTNLRAGDVIFIDEIHRLGRAVEEVLYPAMEDFSLDIVIGKGPSARSIRLKLPK
                     FTVVGATTRLALVTAPLRARFGAVYRLDYYDLEAMKKIISRAAAMLKVESDADGIVEM
                     ARRARGTPRIALRLLRRVRDYAQVRANGVITQKVAREALDLLQVDPLGLDDVDRRVLK
                     TIIEKYSGGPVGLNTIAASISEEQDTIMDVVEPYLLQLGFLDRTPQGRVATKSAYEHL
                     GYTYTEQGQQRLL"
     gene            complement(13668..14684)
                     /locus_tag="PLE39_14045"
     CDS             complement(13668..14684)
                     /locus_tag="PLE39_14045"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69502.1"
                     /translation="MENKTYSKLFVAGFFLLLVAQLFGPKLTLKTISERFLFRLPLIQ
                     NFNSLRYTLGDQVFNEGIVGKDGWLFYSGDFSIHDYQKTSPVSKKNLKEVEAILNQLN
                     DAAMRSGGSLLVVIPPDKNTIYPQYMPDEIPVLGEVSRLEQFMQYMQEHTNIQIMDLR
                     PVLSEASQSTQVYFKSDAHWNCLGAYYASAEIMSLISISHPEVRPRPLSDFEIGTVID
                     STLDISKTMGLGLQEETWVLTPKFETHVEVMPAPFAENKQMRVSENSQTDLPSAVILH
                     DSFYNECLNQLLEPYFSKTYSLHYGTSKLSDYVGLIEKEKPDVVIVEFAERHIEYFFK
                     LLTE"
BASE COUNT         3895 a         3817 c         3617 g         3431 t
ORIGIN      
        1 cttcgggttt tgctccgtgc ttcgcagcaa gcgattcaag ccatgccacg gcatcgtcct
       61 gctcttgtgc ggtggagccg aggctttcaa ccgatggcgc gggcgtctgc gcagcaggtg
      121 tttgttgttc gatgttttga gcctgcgaaa cccattcggg cggggtttcg ctgcgtttgt
      181 tcgggtcggt gaccagttct tcgggttttg ctccgtgctt cgcggcgagc gattcaagcc
      241 atgctacggc atcatcctgc tcttgtgcgg tggagccaag gctttcaacc gatggcgcgg
      301 gcgtctgcga aagcggcgca tcggtgctga tgctatgcag ccattcagtg gcttccccgg
      361 tttgcccaag ttcgtcaggt tgggcgtcag gaatgcccgt catccaggca ggcagatcgc
      421 tgtcactggt gggttgtgac gcggcgggct ggctggtttc acccaaacca ctcatccagg
      481 cgggaagatc atcagattgt gcgggagcag cctgaacatc agcaaacaag gagtcagggc
      541 cattgccgct cggagtttgg gaatcatcaa tatcgcgcag ccaatcaggc acatcgccag
      601 aagcggccgc ggcaggtgca acggatgttt tgggggtatc caaaccgttc agccattcca
      661 tcacatcatc aggttgtgca gatttggcgg cttcagcatc accaaggccg ctcaaccatt
      721 cgggcacatc agaagattgc gaaggtgtgg caggggttgg agcagccaca ggagcgggag
      781 aatcaaggcc gctcaaccaa tctgaaacat catcggtctg agcagcattg gttgtggtgg
      841 gttcatcacc caatccgctc aaccaatcag ggacattgcc cggttgcgcg ggtggaatgg
      901 aacttacagg ttcagaggga gtagcatcat cgccaagtcc gcttagccaa tcagggacgt
      961 tgcctggttg cgcgggtgga atggaacttg ctggttcaga aggagtggcg gcatcgccaa
     1021 gaccgcttag ccaatcgggg acgttgcccg gttgtgcggg cgcaggctca gattgcgcag
     1081 aactggcagc ggcaacagcc cctgcagtac caagtccact caaccagtca ggaatatcac
     1141 cggaagcgga cgatgtctca gagccggtat cgagtccgct taaccaatcg ggagtttcta
     1201 tggattgaga tgaagcaggt tgaggtgcgg caggctgcga gacattcgtc ggcacctgac
     1261 ctttcaacca gtcgggaatg tcagcagggg tcagatcacc gctggcaggg gtatcatcaa
     1321 aaaaggaagt gggctgctcg gggcctgttg attcgcccca acccgccgca cggagaaagt
     1381 ctggaatatc ttcaccagat ttggatgcgc tcgtatcgga agcattcttc aaccaatcag
     1441 gttcgctgtt ggaagaagaa agcatcggcg ccgaagatgc acccgaacca aggccaaggg
     1501 atgaagaaga tgctccccag ctttgagtcg ctggtgcgtc ctcttccttg taatcaagac
     1561 gttccagatt aatggaagca tcgggaactt cattcgcaag aaatacagag cctttggcaa
     1621 aagcagtgta tgggtccaac tcacccacac gcatgcgata cacttgtgta ctttccaccg
     1681 cccccgcagt ggctggtaac aggtccacca tgatgcaatt cgcatcgtag caatacagat
     1741 aacgtttcaa caactgcatg cacatgtcgg aggcatcggc tttttgtccg ccgtggaaat
     1801 atgcacgagc caacaatacc tgcatatcgg cacggtctgg ctcctgtgcc agaacagcac
     1861 gaatctcggc aatggcctgc ggatacaact cgccctgcac atacatgtga gcaagcgcac
     1921 cgcgtgtcat gcggatcttg ggcggctcca tgccatcacg tcggccatag agtctttgca
     1981 attcaccctg aatggcagaa ttggaaggct gtgcttcgaa ggcacgctgc atatgccaga
     2041 tggcatcgtc cagtttgttc tcttcatcac ggatgatgct catccccaca tgcgaaacaa
     2101 agtcatcggg cacagacatc aagacacggc tgaaaatatc cacagcctcg ccgtagcgtt
     2161 tcagttcaag atacgccttg ccaagcaggc gataggttgc cagatgtttg gggaaggtcc
     2221 ttagaatatg gtggcaatgt gctatggctt cattgatgtg agcctgatca atgagtcctt
     2281 cgatctcgcg gttgtaaatt ctcaaagaaa cttttgccat taccgttttc ctgattaatt
     2341 aaggttgcct gatttggggg taattcacga ggctatacgc tattttaccc gtaaaacagc
     2401 ggcaatggcg atacaaattc atcgctaaat gtgtatgccg cgagcaccgc cttgcgcgct
     2461 tcagccaatt tagtctcatc attcgcatgg atcgcgaaca acggctctcc tgcctgcacc
     2521 tggtcgccta cttttttgta gatcaggaag ccaaccgcat ggtcaattgg atcggtcttt
     2581 ttggatctcc ccgccccaag caggaccgag gcctcaccca cgagtctggc gtggacctgt
     2641 gaaatgaatc cgcttcgagg agcattcacc acctcgacat atttagcctg ctcaaatttg
     2701 gaaatgtcat ccacatacga aacatccccg ccctgagcct gaaccagcac acggaatttc
     2761 tccagcgcac tgccatctgt gatggatttc tcggccatca cgcggccttc atccaaatct
     2821 tttgcgcgtt gccccaaaac caacacatgg gcggcgacat gtaaacaatg ctcataataa
     2881 tcaaacgggg catgtccgcg aaggagctca atggcttcga tggtttcaag ggcattcccc
     2941 accgccgaac ccagtggttg attcatatcg gagatcaggg ccacagtttt acggcctgca
     3001 aggcggccaa tatccaccat caaatcagcc agtgtatgtg catcttccag ggtttgcatg
     3061 aatgcaccga gacccgtttt tacatccaaa acaatggcct gtgcgcccgc tgcgatcttt
     3121 ttgctcatga tggaggatgc gatcaacggc atggaaggga cggtccccgt cacatcgcgc
     3181 aaggcgtaca tttttccatc tgcaggggca aggtcgagcg attgacctgt aagcacaatc
     3241 cctttttctt ttagttgctg cttgaattca tcggtggtca ggttcacacg atagccggga
     3301 atggcttcga gtttatccag ggtgccgcca ctgaacccaa gcccgcggcc cgacatttta
     3361 cccacaggca aaccacaagc agccaccatt ggcatgactg aaatgctggt cttgtcaccc
     3421 accccgcccg aggaatgttt atccacggca atatccacca ccttggaaag atcaagcacc
     3481 tgccctgatt ttgccatcgc cagagtcaag tcggcggttt caaagggggt cataccattc
     3541 agcaaaacag ccatggcaaa agcagaagcc tgataatcgg gaatttcccc gctggcaaat
     3601 ccttgaatga aaaaatctat ctcttgcgag gtcaattcct gcttgtctcg ttttttgatg
     3661 atgatgtcta cggcacgcat gaggccaact ccttatgatt tccttctgga atattttatc
     3721 gcagataagc agagaccgcc ctgcctctta ataataaagc ccgaaataaa aacaacaaaa
     3781 taaaaagagt tgcatcaaaa cgatggcaac tctttttata aaagccttga aacacgaatt
     3841 atcctttaat cagcttctga ccttctttgc tgtcttgcac cacccagcct tgtgatgcga
     3901 tctggtcacg caaccgatct gactcggcga aattctttgc aacgcgggcc tgctgacgaa
     3961 gttcaagcag cttcaatact tcctctgaag ggccttcttc tgaagaagct ttattcagca
     4021 tttcccagat ttcgggtcgg attcccttct ccaacggtac aggcagggca caattgccca
     4081 gctcgcttaa tgaaaaactc gcccctgtgg ggtacatttc catgtctttg tttttcatca
     4141 ccgttacgga gctgacacca tgtacatcac aagtgccctg ctcgcaatcc atgatgaccc
     4201 ctgagtgctc atccaatcca acgatgatat tttcgggcgg aagtaatcta tgccattgat
     4261 caaaacgttc caatccaaca aaacagcggc tggtatccag gtcaatgcca ccttcggcgt
     4321 tgttccaatg cggaacgaaa gaaacgttca tgccaaaatc ggagaacaga ttcaaacctt
     4381 cctttgcgtg gacatcctcg ccaactttgt aaatttcata aacaggcaaa gcccatgcgc
     4441 caactgaaat ggtcgcggcg gaggcaaaga cgagggtcgc cccctgtctg tggcgggcgc
     4501 ggatgatatc ccaggcgagc gtgccgcgca attgacgggc ggtgtaagta ggcgaaccag
     4561 gccccatgaa aataagattg gcttgtaaca ggggtttcag aatatcggga ttatcggggc
     4621 tgaattcggt cccctgcttg cgggcgggga tcaggtcaat ggtgggtttg taattttgca
     4681 ggcgggtctt cagaaaatca gccacgcggt ttgcaacgag ggaggcattt aactcaaagc
     4741 cagctggcgt ttccagaatg gcaacccgca acgggtcagg gatgaggcgg gcgagtgtct
     4801 caaagatgcg cccgcccgca agagaggtct cgccagagcc gagaaaggca attcgtccaa
     4861 gtgtcatact aactggatga ttcgtttagt ttatctatta cgttgaggaa agtttcatta
     4921 tcagggatat cagccatgtt ggagccataa tggacataac gaatgcgccc gtcaaggtca
     4981 acgacgcaat ttaaaggcat acgtcccagt ttgaagagat tcacttcctg cttgtagagt
     5041 tttgccacac ggtgaccagg gtcgggcagg ccgatgtatg gcaggctttc attctgccaa
     5101 tagcgttgaa aggcctccac tccatcgggg ccaacggcaa cgatctccac attccgtttt
     5161 gtgaaatcag aataatgatc acgcatgcgc gccaactgcg cgcggcagta tggtcacata
     5221 aagccgcgta aaaatgcaag cacaacaggc ttgcttccgc ggaaactgga aaggcggaca
     5281 gtattcccat gaatatcttc gagttcaaaa tcgggtgcga tttcgcccgg ggtgatgata
     5341 ttgagcagca ttgtttttca cactttcagt agaatttgca aaacgatcat agctccagga
     5401 atttcagaac ttcctgcata aactgctccg ggcattcttc gtgcggcaca tgaccgcatt
     5461 gaggcaataa caccaacggc gcaggggttt cctgtgaaat cgcttcaaaa taccaggtgc
     5521 ggatcaggcg gtcatctgca ccggcaatga taaaagtatc aaccgtcaaa ttcggcagca
     5581 acggacgcaa ctccgggtag gcaggtgcga aagtcaattc gtaaaatgcc cgatcccaat
     5641 tctttatttg aagcgcgttc aaatattcct gacgcacctc cgcgctgagg ttgttttcat
     5701 tggcccattc acgcgtgatc gtgtttggaa gcgtctcttg ataatcgcgc atcatcaatg
     5761 ggccaaggtg tcgcatttgc ggagtccaca tgacaggcaa tgcccacgcg ggaaactgcg
     5821 gaccgtatcc accaccgacg cctgggtcca ccaaaatgag tttgtcaacc cgttcagggt
     5881 aggttagcgc aaaagccacc gaaaccccgc cgcccgctga attaccaacc agcaccgctt
     5941 tttcaatttt caaggtatcc atcaggccgc gcagcaattc cacgttggcg cccatgccat
     6001 acggattttc ctgccacgtt tctggcatcg gacgctctgt cagcccaaag gcaggacggt
     6061 cgtacgcaat gacacggcca tactttgaaa aatcatccat cacttcgcgc catgaaaaaa
     6121 cgctcgagcc aaatccatgc aaaaggatga aagtggtatt gccttcaccc ttttctttgt
     6181 aatgcaccgt aacgtcgttc acctcgataa atttactatc agggtcagcg agctcggctt
     6241 cagtcacaag tccctgtaat tctggcacag gtacaagaaa aggcccaaac agcacaacaa
     6301 ggcaggcgaa aagaaatatt ctaaaaagaa tctgcttcca acgtttcata atcaattcaa
     6361 cagacttgat accaataaag aaagattaat gcaccagctc ctcaaggaat ttcacaacag
     6421 cattgaccac ctgtgtctgc tgttctgtgc gggagatggt tgcctgtttg tcaccgggct
     6481 ggggaccata gtcgccaaat tgggcgtggt tacctccctc aatcacaacg tactcggtgc
     6541 ttgcgggcag caaggcgctt gtttcactaa actgttccat ccctgccata tcgttcgtgc
     6601 cataaatgga caacatcttc aatgaactat tcttcaacga atcatctgca gggtacgaag
     6661 cccaaaagac gatcccatcc accgtatcca aatgatcctt tgcgaacaaa gccgatgcca
     6721 caccgccaag ggaatgacca cccacaaccc attgctggat ctcgggatgc acagcaaaca
     6781 ccgggtcacc tgcattgaca tcaaaaaaag ccaaattcag ttttaccggc agcaacacca
     6841 ccagatatcc ctgtgcagca atcgcacgga gcggcacagc atatgaacgg taatccacat
     6901 ggccgccggg gtaaaagata aaaccagtta caggtttaat atttacaggc tcaaaagtga
     6961 tggcatcacc gatcgtaaca gtgacatttg aatcgctttc caaggcagcc aatgcctcag
     7021 gtgcagggcc aagcggggtt tctgcccaga tcacaaatcc caatacagcc aatccaacga
     7081 caagtacaag tccaaacaga acacgcttaa tgattttcat tgaggagcaa tttcgccttt
     7141 tcccaaattt cgtcgaacat ttttacggtc agttttccag tcaacgtgtt ttgctggctg
     7201 gggtgatagg agcaaatcaa agatgaagga tgatttatga acgatgaagg caggagcgtg
     7261 tgaaccgctc catgtgcgaa cttcaaccct cgagttgcag ttattttgag tattcgatca
     7321 aaagcaatct tacccagaca cacaattaca ctgggttgaa tcagttgaag ttctctttcc
     7381 aaaaacggct gacaggtgtt caactcttct ggattcggct tattctctgg cggggcgcaa
     7441 cgacctgacg ccgtgatgta catatccttc aaaatcagac catcgcttct ggactccgcc
     7501 cgggcctgat tcgcaaaccc agcccgatac aacgctggga aaaggaatcc gcccgaagca
     7561 tctcctgtaa attgacgtcc tgttcggttc gatccgtgtg cgcctggggc aagccccacc
     7621 accagcacac gtgctttcgg gtcgccaaaa cctggcacag gcctgcccca atattcatca
     7681 tctttataag ccttgcgttt gacccgcgcc acttcctcgc gccattccac caagcgcgga
     7741 catttgcggc aggaaatgat ttcttcgttt aatgtttcaa ggttggtctt cattgggttt
     7801 caggttgaag gttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatttctt
     7921 cgtttaatgt ttcaaggttg gtcttcattg ggtttcaggt tgaaggtttc aggtttcagg
     7981 ttgaaggttg aaggttgaag ggttcaagta gccaaccttc gaactcttaa tcaatgaaca
     8041 tggcatcgcc aaaggagtaa aaacggtacc cctcgcgcac ggcaaggttg taggtatcca
     8101 aaattctctc ccgccccgca aaagcgctga ccagcatgat cagtgtggat tttggcaaat
     8161 gaaaatttgt gaccatcgca tccacaacct tgaattggta gccggggaga atgtagatcg
     8221 aggtgggacc ggtaaaagcc ccaatgtccg aggtgcgatt tccaattact gatcggcttg
     8281 cggcactttc gagagtccga actgaggtgg tgccgacagc aaccacccgc ccgccatttt
     8341 gcctggtttg attgatgagg tcagcggttt cttgcggaag ctcgcaccac tcggtgtgga
     8401 tcttgtgctc ttcaggagac tcttcagtga cgggggcaaa ggtatccaaa ccaacatgca
     8461 gggtgacgta ccccaccttc acacctttgt tttgcagtgt atccagcagt tgcggtgtga
     8521 aatgcagacc tgcggttggt gcggcggcgg agccagcttc ttttgcaaac acagtctggt
     8581 aacgctcggg gtcgctcaat ttttcgtgga tgtatggcgg cagagggata ttccccacct
     8641 ttggaaaata cggctcaatg gcttccgaaa aacggatcaa ccgttcagaa ccttcgagga
     8701 tttcgataat ttcggcttgc agaaaattat ccgctctacc cgcctcttca acttccacaa
     8761 ccaccttttt gcctacacgc aaacccttcc cgcccaccaa ggcttcccat gtgaccgtat
     8821 cgcggcggcg caacagaagc aattccactt tgccgcctgt ttcctttttg gcaaaaattc
     8881 tcgcagggat gacccgagtc ctgtttagga cgagaagatc tttgggttgt agaaacaggc
     8941 tgaggtcccg aaagacgcgg tgttccacat ccccagaatc acgatgcaac acgagcaatc
     9001 gcgaagaatc gcgcggatca gcaggagtct gcgcaatgga ggattcgggc aatacgtaat
     9061 caaaatcgga tgttttcatg taggttcgtt tcgaattatt tatgaaattt cctggttcat
     9121 atcgcgaacg tgtggagcga gcacgagtgc aaggtagatc atccaaatga tgaggccgat
     9181 atgcaaaaca tcattttcag aaaaccaaaa tcctttttcc cacagcaccg ttgtgttgcc
     9241 tgaaatgaga tacagaaaat aagcggcaat ggtgagtccc agccataacc aggctcccaa
     9301 tgccacaaga tcagatttca gtttgtgttt ggcatatcgc cagccattga tcacaaaaaa
     9361 tgcgatcaca cttggcgcag cgacaatgag aagcagttca aaagaaatta aaaatttgac
     9421 agggataaaa gcgcccgcca tcaccacggc aaaataaaaa accgcattga caatggaata
     9481 tacagaaagg attttgcgca gtttcccatc agtgctggta aaagcgacag ccaacatcat
     9541 cgcatcaatg ctccagacag aaacgatgag gtaggcgatc tcccaccaac ttgtccacaa
     9601 acaggccgta cggccttcac acttgatggc atagctaaag gcttcataac ttgtgcctgc
     9661 caaaagcgcg ccgattcccc atagaagcag ggcaactccc caccaaaaac gggaacgttg
     9721 cccatcgcga agtttcagaa agtacagccc gactccgatg gtcaacaagc cgagaaaata
     9781 aacgatgatc gaagtactcg gttgaacaaa cacgaccttt tgcgaagcaa tttgaaattc
     9841 agaaaacggt tgtatctgaa gccatgtttc gggggtttgt tgcggcgtat agaggatcga
     9901 atccaatcgt ccacaggcag aaagaagaaa aaagagagga agcaggcgca gggaaatttt
     9961 cataaaaatg acagtttggg cgttttcaaa aagcgctcgt tgaagttcga atgataatgg
    10021 attaaaatga aaagtgaaag cacgaccgta tcacggaggc aacaatgaaa ctcagttttc
    10081 acggggcggc ccaaacggta actggctcga tgcatctggt ggaaatcaat ggttcgcgtt
    10141 tgcttttgga ctgcggattg tatcagggga aaagaaaaga atcgatcgaa aagaatagaa
    10201 attttccctt tgaccccaaa accatagatg cggtaattct ctcccatgct cacattgacc
    10261 attcaggcaa cctgcccaac ttgatcaagc aggggttttc aggcctgatc tattgcacca
    10321 gtgccaccgc agaattgacc gacatcatgg ttagagattc agggcatgtg caggaaaagc
    10381 aggcggaaag caccagctat cacaacgcaa agcgcggcga accacctgtt gatccgcttt
    10441 acacggaaga agatgccaaa gaggtcgaac cgctgttgca gccagtccgc tacgatgtgc
    10501 cgttcgagcc tgccaacggt gtgatcgcca ggctgcagga tgcaggtcat attcttggtt
    10561 cggcttccat ctctctgcat ttggaagaaa aaaggattcg aaaacattta tggttttcag
    10621 gcgatatcgg ccgtagaaat atgccgatcc tgcgcgatcc tgttctgcca aaagatgatg
    10681 tggatgttct aatgatggaa accacctacg gcgacaaacc acaccgtgac ccggaagaag
    10741 cctacaacga aatgcgcctc gtcatcaaac ggacacttga tcggggcgga aaggtcatta
    10801 tcccctcttt tgcagtcggg cgcacccagg aacttgtgta caacctcagc cgcatgtacg
    10861 atgaaggcga tcttcccaac gtacctgtgt atgtggatag tcctctggcg gtcaatgcgt
    10921 cgaaaatatt ccgcaagcac gaagagtgct acgatgccga aatgaaagca ctggtgcatt
    10981 cgggtcaccc cgcactggat ttcaaattac tgacctacat taacagcctt gaagaatcaa
    11041 aagagttgaa tgcacgcacc gaccccatga tcataatctc cgcttcggga atggcagagg
    11101 cagggcgcat tgtgtatcac atccgctggg gaatcgagaa caagaaaaat acgatcatga
    11161 tcgtatcgtg gcaatcaccc gaaacattag gacgaaaact ggccgagcgg gagccaaatg
    11221 tgaaaatatt tggcgaaacc taccgctgcc gcgcagagat cgtcaccatt ggcgggctgt
    11281 cggcacacgc cgggcaggat atgctgcttg agtatgcttc gggggtcagg caaaaagctg
    11341 agtatatatt cctcgtccat ggagaggata agggagcaat tccattacga gagaagttaa
    11401 gagaaaaaaa tataaaacaa atttattatc ccgccatgca cacaagcgtg gaactgtaaa
    11461 acacattact tgttcatcaa tttcgagatc aggttcagaa gcaaagacag gatcaccgaa
    11521 atcaaaatgg aacttgtgac agggaagtaa aaacttccat tctccccttc aatgcggatg
    11581 tcaccaggca ggcgcccaag cggaatcccg aaccttgccg caagatacac tcccccaccg
    11641 atcaggaata gaatgattcc gccaagcatg agatagcgnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnga tacactcccc caccgatcag gaatagaatg attccgccaa
    11821 gcatgagata gcgtgccacg gattccacga attacaccct gccttgcaaa ataaatcctt
    11881 cgatctcccg caggacttgc tccaatgaag gaacctcgga ggaagttacc ccaccaccca
    11941 ttgtgtgctt gtgaactgga aatgtgggaa ctgaccgatg atgagccgtg ttatcatatc
    12001 gaaaaatcag gtcaccatcc gcattttggt agtgatacgc atacattatc ttttgaagtg
    12061 ggggacgcaa atcaattaat tcacgaaaga aaagcaggga agagccatca cgaaattcaa
    12121 cttctccgcg aataaaacca gccaaatcac cgcgtttttc aagatttacg ctggacgatt
    12181 ctacaaatgg cagggcagcc agcagatcca gcatggactg gaaatactgc tcaatcaatt
    12241 tttgcctcgc taagcgattg atattgtcct tccaacaaac gcagggcagc gagttccata
    12301 ttccaatcta tgatttccac tgtttcttca acttcattgg actgcagttt tttctccaat
    12361 tcagccgaag acatccccgc ccgtttctca aattcagcta ctcgttcttt ggtacgttga
    12421 atgccgtgca acagggactt caattggttt tgaatcgcaa cctccaacaa tggcttgagc
    12481 gcctcattgg aatctgtttt gataatgact tcctgcaaca tagcttttcc tttctttaaa
    12541 ccatctgcag atgttacaac aacctctgct ggccttgttc tgtgtaggtg tatcccaaat
    12601 gttcataagc cgatttggta gcgactcggc cttgcggggt acggtctaaa aacccaagct
    12661 gcaacagata tggctcaacc acatccatga tggtatcctg ctcttcgctg atggatgcgg
    12721 cgatggtatt caaacccaca gggccgccgc tgtatttttc gatgatggtc ttcaaaacgc
    12781 ggcgatccac gtcatcaagg ccaagcggat ccacctgcaa caggtcgaga gcttcacggg
    12841 caactttctg ggtgatgacc ccattggcgc ggacctgggc ataatcacgc acgcggcgca
    12901 aaaggcgcaa ggcaatacgg ggagtccctc gcgcacggcg cgccatttcg acgatcccat
    12961 ccgcatcgga ttccactttt aacatggccg cggcacgaga aatgatcttc ttcatggctt
    13021 ccagatcgta ataatccaaa cgatagacag caccaaaacg tgcccgcagc ggagcagtga
    13081 ccaaagccaa ccgcgtcgtg gcgccgacca cagtaaactt cggcaatttc aaacggatcg
    13141 aacgcgccga tggccctttg ccaataacga tatccaggga aaaatcttcc attgctgggt
    13201 acaaaacttc ttccacggca cgcccgaggc ggtgaatttc gtcaataaag atcacatcgc
    13261 cagcacgcag attggtgagg atggccgcaa ggtcacctgc acgctcgatg gcggggccag
    13321 ccgtcacttt cacgttgacg cccatttcat tccccagcac atgcgcaagg gtggtttttc
    13381 ccagaccagg cgggccataa aataaaacat gatccaatgc ctcgcctcgt gcccgagccg
    13441 catcgatcaa aatggacaaa ttttccttga cctgatcctg gccgatcaaa tcgtccaact
    13501 tttgcggacg cagggcgttg tctacgcggt catcgggttt ggattcaggg tcaagggctc
    13561 gccctgaatt ttttgaagag gaaggggatt tgctcatggg gagattatac cctgcgggta
    13621 gaagctatca ataaacaacg atccgttttc agttaagcgc gaagcggcta ttccgtcagc
    13681 agtttgaaaa agtattcgat atgtcgttct gcaaattcaa caatgaccac atcgggcttt
    13741 tccttttcga tcaggccaac ataatctgaa agtttcgaag tgccatagtg cagggaatat
    13801 gttttgctga aatacggctc cagcaactgg ttcaaacatt cgttgtaaaa cgaatcatgc
    13861 aaaatgaccg cactgggcaa atcggtttgc gaattctctg aaacgcgcat ctgcttattt
    13921 tcggcgaacg gggcgggcat cacctcaaca tgggtttcga atttcggggt caagacccac
    13981 gtttcctcct gcaaaccaag ccccattgtt ttggaaatat caagcgtgga atcgatcact
    14041 gtgccaattt caaaatcgga taaagggcgc ggtcgcactt cgggatgact gatactgatc
    14101 aacgacatga tctccgctga ggcataatac gccccaaggc aattccaatg cgcatcagac
    14161 ttgaaataaa cctgcgtgga ctgactggct tcactcagca cagggcgcag gtccatgatc
    14221 tgaatgttgg tatgctcctg catgtattgc atgaattgct caaggcgcga gacctcacca
    14281 agcacgggta tttcatctgg catgtattgc ggatagatcg tgttcttgtc tggcgggatg
    14341 accaccagca ggctcccgcc gctgcgcatg gcagcatcgt taagttgatt caggatcgct
    14401 tccacttcct tcaaattctt tttactgacg ggggaggtct tttgataatc atggatgctg
    14461 aaatcaccag agtagaacag ccatccatcc ttgcccacga tcccttcatt gaagacctga
    14521 tcgccaagcg tataacgcaa gctgttgaaa ttctggatca atggcaggcg gaataaaaaa
    14581 cgctccgaga tcgttttgag cgtcaatttt gggccaaaaa gttgcgccac cagcagaaga
    14641 aagaagccag cgacaaatag tttggaatag gttttatttt ccatcgcggc aataataaca
    14701 gaatcttgag gcgagataca aaaataagag tggctaaacc cgtggggtaa acacagagac
    14761 gccaagccac taagaatcct ttgtgtcttc gaggctttgt gactttgtgg caatcaaaac
    14821 ccactctttt agccactctc aaaaaaataa ctcacccacg aaataaacat ttcggggtga
    14881 gtcagatcca tttaaagggt tcagttatgc aagcaggaat ttccgcattt cagcatggac
    14941 cagttcctct tgagccatga
//
LOCUS       DAOQJC010000100        14845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1923_length_14845_cov_10.727248, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000100 DAOQJC010000000
VERSION     DAOQJC010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14845
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1923_length_14845_cov_10.727248"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2688)
                     /locus_tag="PLE39_14050"
     CDS             complement(<1..2688)
                     /locus_tag="PLE39_14050"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE69503.1"
                     /translation="MSLQEPSQKPDPFSDKDAFNLLFENHPTPMWIHDSKTLALLDVN
                     EAAQQLYGYPHEEFLSITLKEIYAVTEMPADSESLTGEFQHRKKNGQVIHVEVTLRTI
                     QYGGQNAVLAVIKDLTKQYDQLYLRISALNAAANAIVITDTNGMIEWVNPAFSTLTGY
                     TFEEALNKNPRDLIKSGEHEPGFYKTMWSTILSGKVWHGEVINRRKDGSLYDEEQTIT
                     PLKDDNGKITHFISIKQDISQRKRTEKIVNQWLAEQNTLHQVSQSLLSIHLDSDMIYS
                     TLHEAVVQIMPCDVFTIVLDDKGNGDYHAVYLYDLEERFPPRRVPRGQGLSGQVISSG
                     QTLFIHDNFEARTPLIRFGTPNEVRSILAVPLRKHRKTVGMISVQSYQPNVYGERHRV
                     LLETLAAQATTTIENASLFAETRQRLVELELLYESGLALNHLLNPKELGTKIVELLEQ
                     KLGWHHITIRLTRPQDDGLELLAFKQAGLTDEVEKEKVAERFQALIQHIGQGLSGWAI
                     EHQQVVRVDELSNDPRYVDTYPGLHSGLYVPMKLGERIIGVISIESELPNAFSEADER
                     LTATLANQAASAIENARLYEAERKQHRMSDALRDALHAGALMSTSLDFETIMDRLLET
                     VKRIVPFAGGTMMFVDHEQRRVRITKMHGYKMLKKEMIEKISSYSFDLSTAENLRWML
                     DNKQPLVISDVRQYPGWVRIPESEFIRSWLGAPIIINGQVIALFSLDSTETNFFTEEH
                     AELLQAFTGQASLALQNARLFDETAQRALEFASLYQTSSFLSAENELDPLLQNIVESA
                     KKLLNSNSSGIYLYHPEDEELELSVITTNDILTGVRLRLGEGVAGIVAQTHQPLRIEN
                     YSEWEGRSSKYDELRIRAVLEVPMLYGGELIGVLTADEIN"
     gene            complement(2688..>4094)
                     /locus_tag="PLE39_14055"
     CDS             complement(2688..>4094)
                     /locus_tag="PLE39_14055"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE69504.1"
                     /translation="ETHMIEFDGRAAALIMVKPAMPTQAVENQSESELELRALFASMQ
                     DVVLVIDHDGVYRKVAPTNSELLIKPKSELLGKNLSEFFPAERAEFFLKSIRQVLETR
                     QSTHIEYEISLEYGTIWFAATISPMDENNTLWVVRDITERKMVNKALQESEGRYRSLF
                     DRMMDGIYRSTHEGKFVDVNPAMVKMFGYSSKEEMLAIDIKTELYFDAEERGTHKLDT
                     GDGETDIYRLRRKDGTEIWVEDRGSYTYDDHGNVIYHEGTLRDVTARKHAEEALHRAK
                     ESLERTNQELERVLQREQQLARTDGLTGLYNYRYFFELATREFNAAMRYRRPLSILMF
                     DTDDFKRINDTLGHSAGDKILALIAQATAAQMRSVDVLARYGGDEFIILLPQTSAKQA
                     YPIAERIGNSVADMQMPSDKGPVSVTLSIGIAEIQYEPADESVEFIVQRADKALYAVK
                     QAGRNRAMIYTASLDGEN"
     assembly_gap    4095..4194
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4439..5695)
                     /locus_tag="PLE39_14060"
     CDS             complement(4439..5695)
                     /locus_tag="PLE39_14060"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNE69505.1"
                     /translation="MDTISRITFRRYLLGQQGLWPGRRFKGLAGTTSAIQQMEGIQLD
                     PLNIVARSQEIALYGRVLDFKPENLYQAAYEKRGFFDYGGWLFMYPMSEFPYWRLHMK
                     HRKKQGIYYHDAYQQPPAKLLNAVLNELRTRGPLGNRDFSGEALKSWSYRGRKEESIA
                     LFYLWLIGEVMITNRKGFDRIYDLTERVLPKKYEHTVPDSEAEDFFSRKTIAHLGLAR
                     ENVWRTGLAGFIHRDVSREEGKKRIERLIEQGIASRLRVEGSKDGYLVLNSEQSHLSD
                     LEAGRIPKKWKLLDTSTQEEVTFLAPLEMVSARGRAKVVFDFEYLWEVYKPLHQRRWG
                     YYTLPILYGDDLVARLDPKLDRQTETLHILGFWLEDDAPKDEAFASALAKGLARFARM
                     IRAKKVDVSAVRPPKLRSYLKKNIGL"
     gene            complement(5749..5940)
                     /locus_tag="PLE39_14065"
     CDS             complement(5749..5940)
                     /locus_tag="PLE39_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69506.1"
                     /translation="MNNSKNILLGALIGAVTGLIAAMLLTRRAEKNERETALTAGEGL
                     KLGVLIFGLLRAIASLGDD"
     gene            complement(5937..6365)
                     /locus_tag="PLE39_14070"
     CDS             complement(5937..6365)
                     /locus_tag="PLE39_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69507.1"
                     /translation="MKAQLPHPEHYSYLVHRKQRNTQVILPVVLSALLMIGMIVLISF
                     ATFKSGGDVGRWAAISTIWIIIPLLITGLIVLALLIGGIYLMARALSGLPYYTGIAQD
                     YVHKARGYITRGADMAAKPILAINDLVENLKAFFGRISNS"
     gene            complement(6414..7157)
                     /locus_tag="PLE39_14075"
     CDS             complement(6414..7157)
                     /locus_tag="PLE39_14075"
                     /inference="COORDINATES: protein motif:HMM:NF012420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNE69508.1"
                     /translation="MKKLLIIASLLLGACANQPQEAPHWTYEGEEGPQHWGEIDPSYA
                     VCGTGENQSPIDISKPIEKDLVNISFHYQPSEVNILNNGHTAQVNYDTGSYIELDGTR
                     YDVVQFHYHAPSEHHIDGQAFAAEIHVVHKNAEGKLAVIGILLQQGTENAAFDPFVNN
                     LPAEKTDAKDAGSKVNAADFLPAEQTTFRYSGSLTTPPCSEGVSWLVMTSPVEVSAAQ
                     LEKLLTVFEGNNRPLQALNERTPLEDSTP"
     gene            complement(7418..7882)
                     /locus_tag="PLE39_14080"
     CDS             complement(7418..7882)
                     /locus_tag="PLE39_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE69509.1"
                     /translation="MSKKKNRNTTSRSGLSAFLRKPAGQLTLIAVVVIVIVLIALSGQ
                     GSGQGLSREVNTDQAYEMYQNGAFVVDVRTQEEWDEYHAPNTTLIPLDQLQSRLAEIP
                     KDREILVVCRSGNRSQQGRDILLAAGYNATSMAGGLKEWYAKGYPVEGKPAQ"
     gene            complement(8067..8477)
                     /locus_tag="PLE39_14085"
     CDS             complement(8067..8477)
                     /locus_tag="PLE39_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE69510.1"
                     /translation="MQIRITKQEWRVFVTIMHLSGNLDASNYIEVINKAQELYASGVR
                     DILIDLSGVSYVSSAGLMSLHTVVLIFAGQSMYSKETGRPSFRAINFERDGAVRQHVK
                     LFGPQPSVEQVLDVVGLKQFFDVYHDLDAAVKSY"
     gene            complement(8510..9787)
                     /locus_tag="PLE39_14090"
     CDS             complement(8510..9787)
                     /locus_tag="PLE39_14090"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNE69511.1"
                     /translation="MKKKNIGNLTIIILVALTLIIWIVFPPVAEGAEHYARAYAGEVL
                     GSLVIVLMSFSLFISTRPKWAEPFFGGLDKMYMTHRHTSTSAFLLMFVHLLTVPITLV
                     NLRVGNYLAMVAFLGIVAIVLPTLAPRIPFLSKLTGQTYEGWKKLHRFVGIFFILGFV
                     HSLTINAPSAKIAITWTQNFFILGTLSYLYTEVFGGLFKKYLPYTVEAVNHPNNSTTE
                     VVLRAKKESIKNQKAGQFLFVHFAGEGLDEAHPFTISSAPHEDVLRVTIKACGDFTRH
                     LFANLKAGMSATVEGSYGMFDYKSGGPKQIWIAGGIGITPFLSFIRDLKTDPAFDVDF
                     YYTVRHREEAIFVEEIQTAAQKYPRLKTHIRFSSTDGSLTVEEIAKNAGGDISTHHVY
                     MCGPLPMVQAFEAKFIEMKVPADNIHFEEFNFR"
     gene            complement(9866..10276)
                     /locus_tag="PLE39_14095"
     CDS             complement(9866..10276)
                     /locus_tag="PLE39_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE69512.1"
                     /translation="MEITISQEQGRISVAVIKLAGHLDGQTYQTLIAKAREVVEAGTR
                     NILLDLGDLTYISSAGLVSLHTIALMTRGEELPDLEHGWATLKSMDRSRDGGMQKNLK
                     LLNPRPEVISVLDMVGFSAFFETFTDRQKAIDSF"
     gene            complement(10308..11546)
                     /locus_tag="PLE39_14100"
     CDS             complement(10308..11546)
                     /locus_tag="PLE39_14100"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNE69513.1"
                     /translation="MDTIHPMSSLADKLKALGVKTGTADLPAPKPASRSIDSVVAGTF
                     FQTPRGEAFIAEQTFAEDYLHGKTAYASSFPFSIIAQWANDPRMAELPIHKFAFLDTE
                     TSGMAGGTGTYAFLVGAGRFVDGRFVLRQFFLRDPSEEPAMLEALIDFLAPCEGLVTF
                     NGKAFDAPLLVTRYSLHRIPVPFKDYAHLDLLPLARRLWRDRLPSRALKYLEEHVLGF
                     TRTSEEVPGYEIPWLYFDYLRTGDASPMAGVFYHNAMDVVAMAALLAHVSELLADPYN
                     GRVEHGLDFIALGKLFEDLGHWDEAARLFERGLELGLNESDFGVAVKRLSILQKKRGD
                     ADQAIRLWEQAAQRGHVYAHIELAKYYEHKMRDAETSLKWARSARAEVERADLPPYIR
                     KHWLGEIDHRLQRLERKAGL"
     gene            11638..11841
                     /locus_tag="PLE39_14105"
     CDS             11638..11841
                     /locus_tag="PLE39_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69514.1"
                     /translation="MVKVNYQYEKRKKELDKKRKKEQKLKLKQQKKNNPSGEVLTEVQ
                     DGAMVEAPAETMPVTPPVEEVDK"
     gene            12016..12792
                     /locus_tag="PLE39_14110"
     CDS             12016..12792
                     /locus_tag="PLE39_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69515.1"
                     /translation="MNRSLNLQLKLLPFLKSASLKRGAIFGGILISALLAFELFNFGT
                     TSFALQDVLGNLKFAGIQWSAILAFAFCGIDFAGIARIFTPEQGRDEPAEVWYLFGAW
                     LLAAGFNAMLTWWGVSVAILNHNAEGGSLVGQAVMVKAVPIFVAVMVWLIRVLIIGTF
                     SIAGERLFSLAEATSRPAATAYQNNSPRPVTQSQPVYRPAAMSASSSNQNYPRPAPKP
                     RPAPVSPNYGANRPEPTYHPIGMTAMGHGEGSNSTSSVRR"
     gene            complement(12899..13777)
                     /locus_tag="PLE39_14115"
     CDS             complement(12899..13777)
                     /locus_tag="PLE39_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69516.1"
                     /translation="MNNFSIRLIETPEEMRLVEDLQRDVWPGSETDVVPLHMLITAVH
                     NGGLVLGAFVEEKIVGFVFGFPGLESTPDGPRPKHCSHMMGIHPDYRDGGVGFSLKRA
                     QWQMVRHQDLDHVTWTYDPLLSRNAYLNISKLGAVCVTYRRSEYGDMRDGLNAGLPSD
                     RFQVDWWINTPRVERRLSKRPRPTLKLSHVASSGLRQFYPLRTSTNGLPQPPEHVPPF
                     DDRLLLAEIPSDFTALKSQDFALARDWRFFTRELFETAFEKNFIVTDFVFDPNNGTPR
                     GLYILTHGESTLDEFE"
     gene            complement(13780..14733)
                     /locus_tag="PLE39_14120"
     CDS             complement(13780..14733)
                     /locus_tag="PLE39_14120"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNE69517.1"
                     /translation="MILITGATGFIGRTLVRQLSSIGYPLRALIRPSPRTPNLPKGVP
                     VEVAVVSLADKRGLRAAMRDVEAVIHLASAENQGSRGNLLTADIQGTENVVEAAADAG
                     VDRIVYLSHIGAARSSGYPAFRAKGIAEEHIRNGKVPYTILRSSLVYGPEDHFTNNLS
                     RLVRASVGLFPIQSGGRTVVQPVWVEDLATCILWSLENEDTLNRVYELGGSEFFTLQQ
                     IIETIMDVTRQRRVLVPLSPITLRAITVFLDGVIPNFPISSFWLDYFAVNRTCAVDSM
                     PRVFGLMPARFTYRLDYLIRKPWYQRAWNTLTERIAALRTR"
BASE COUNT         3377 a         3617 c         3962 g         3789 t
ORIGIN      
        1 attgatctcg tcggcggtca gcacaccgat cagctcgccg ccatacagca ttggtacttc
       61 aagcaccgca cggatgcgaa gttcatcata tttggaggaa cgtccctccc attcagaata
      121 gttctcgatt cgcagcggtt ggtgggtttg tgccacgatt cccgccacac cttctccaag
      181 ccgcaggcgg acgcccgtga gaatatcatt tgtagtgatg accgacagtt ccaattcctc
      241 atcttcaggg tggtaaaggt agattccgct tgagttggag tttaataatt ttttggcgct
      301 ctccacaatg ttttgtaaca gagggtccaa ttcattttca gcggaaagga aactgctggt
      361 ttgataaagc gaagcaaatt ccagtgcccg ttgggcggtt tcgtcgaaca ggcgtgcatt
      421 ttgcagcgcc agagaggctt gtccggtaaa ggcctgcagc agttctgcat gttcttctgt
      481 aaagaagttc gtttctgtgc tatcgagtga aaacaaggcg attacctggc cgttgatgat
      541 gatcggcgca ccgagccacg aacggatgaa ttcggactct ggaatgcgta cccagccggg
      601 gtattgcctt acgtctgaaa tgacaagcgg ttgtttgtta tcgagcatcc aacgcaggtt
      661 ttccgcggtg gacaggtcaa aactatagct tgatattttt tcgatcattt ccttctttaa
      721 cattttgtag ccatgcatct ttgtaatgcg gaccctgcgt tgttcatgat cgacaaacat
      781 catggtgccg cccgcaaaag gcacgatgcg tttgaccgtt tccagcaaac ggtccatgat
      841 ggtttcgaaa tcaaggctgg tgctcatcaa ggcacccgca tgcaaagcgt cgcgcagcgc
      901 atcagacatt cggtgctgtt ttctttccgc ttcatacaaa cgggcgtttt cgatcgcact
      961 ggctgcctga ttggccagtg tcgctgtcag gcgttcatct gcctcactaa atgcgttggg
     1021 aagttcgctt tcaatgctga taacaccgat gatgcgttca cccaatttca tggggacata
     1081 gagaccggaa tgcaggccgg gatatgtgtc cacgtaacgc gggtcattgc tgagttcatc
     1141 cacacgcact acttgttgat gttcgatggc ccaaccgctc aagccctgac cgatatgctg
     1201 gatcagcgcc tggaatcttt ctgccacctt ttccttttcc acttcgtctg tcaaccctgc
     1261 ttgtttgaat gccagcagtt caaggccatc atcctgcgga cgtgtcaggc ggatggtgat
     1321 atgatgccag cccaatttct gttcgagcaa ttcgacgatc tttgtgccca attcctttgg
     1381 attcaaaagg tgattgagcg caagtccgct ttcatagagg agttccaact ccaccagcct
     1441 ttgacgggtt tcagcgaaca ggctggcgtt ttcaatggtg gttgtggcct gtgctgccag
     1501 ggtctcaagc agcacacggt gacgttcgcc atacacattc ggctgatatg actgcacaga
     1561 gatcatgcca accgttttac gatgtttccg caaaggcact gccagaatgg agcgcacctc
     1621 gttgggcgtg ccgaaacgga tcaatggcgt gcgtgcttca aaattgtcgt gaatgaaaag
     1681 ggtttgtccg ctggaaatta cctgtccgct tagcccctgt ccacgcggga cacgccgcgg
     1741 tggaaatcgt tcctcaagat cgtataaata cacggcatgg taatcgccat tccccttgtc
     1801 atccagaacg atcgtgaaaa catcacaagg catgatctga actacagcct catgcaaggt
     1861 ggagtagatc atgtctgaat ccaggtgtat cgaaagcagg gactgactga cctgatgcag
     1921 ggtattctgc tcagccagcc attggttcac gatcttttcc gtgcgtttgc gctgggaaat
     1981 atcctgtttg atcgaaataa aatgagtgat cttgccgttg tcgtctttaa gcggcgtgat
     2041 ggtctgttct tcgtcgtaca ggctgccatc tttgcggcgg ttgatcactt caccatgcca
     2101 gaccttcccg gaaaggatgg tgctccacat ggttttatag aaacccggtt catgttcgcc
     2161 agatttgatc aagtcacgag ggttcttgtt aagggcttct tcaaatgtgt agccggtcaa
     2221 agtactaaac gccgggttga cccattcgat cattccattg gtatcggtga tgacaatggc
     2281 attggccgca gcatttaatg ccgagatgcg cagataaagc tggtcgtatt gctttgtaag
     2341 atcttttatc actgccaaca cggcgttttg accgccgtat tggattgtcc gcagggtaac
     2401 ttccacatga atgacctgcc cattcttttt gcggtgttga aactcaccag tcagcgattc
     2461 agaatcggct ggcatttccg tgacagcata gatctctttg agcgtaattg aaaggaattc
     2521 ctcatgggga taaccatata attgctgggc cgcttcgttg acgtcgagaa gcgccagcgt
     2581 ttttgaatca tggatccaca tgggggtggg gtgattctcg aacagcaggt taaaggcatc
     2641 tttgtcagag aaggggtctg gcttttgtga tggctcttgc aggctcatca attttctcca
     2701 tccagagagg cagtgtagat catggcacgg tttcgcccgg cctgcttgac agcatacaat
     2761 gccttatctg cacgctgaac aatgaactcc acactttcat ccgcaggttc gtactgtatc
     2821 tcggcaatgc caatgctgag tgttactgag accggtcctt tgtccgacgg catctgcatg
     2881 tctgccacgc tgttgccaat ccgttctgcg atcgggtagg cctgttttgc gcttgtttgt
     2941 ggcagcagga tgatgaattc atcgcctccg tagcgggcca gcacatcgac ggaacgcatc
     3001 tgcgctgctg tggcttgagc gatcaacgcg aggatcttat ccccggcaga atggcccagg
     3061 gtgtcattga tgcgtttgaa atcatctgtg tcaaacatga gaatggaaag cggacggcga
     3121 tagcgcatgg cagcattgaa ttcgcgggtg gcaagctcaa agaaataacg gtagttgtac
     3181 aagccggtca acccatcagt gcgggcaagt tgctgctccc gttggagcac ccgttcgagt
     3241 tcctgattgg ttcgttcgag ggattccttg gcgcgatgaa gcgcctcttc ggcatgtttg
     3301 cgggcggtga catcacgcag ggtaccctcg tggtaaatga cattcccgtg atcgtcgtag
     3361 gtgtagctgc cgcggtcttc cacccagatc tccgtgccat ccttgcgcct cagcctgtag
     3421 atatccgttt ccccatcacc ggtatccagt ttgtgggtgc ctctttcctc tgcatcgaaa
     3481 tagagttcgg tcttgatatc gatcgcgagc atttcctctt tactggaata accgaacatc
     3541 ttcaccatgg ccgggtttac atctacaaat ttgccttcat gtgtgctgcg gtagatgccg
     3601 tccatcatgc ggtcgaacaa agaacggtag cgcccttctg attcctgaag agccttgttc
     3661 accattttcc gttcggtaat gtcacgcacc acccacaggg tgttgttctc atccatcggc
     3721 gatatggttg cagcgaacca gatcgttcca tattcaagcg atatttcata ttcaatgtgt
     3781 gtggattggc gggtttccaa aacctggcgg atggatttaa gaaagaattc tgcacgctcg
     3841 gctgggaaga actccgaaag atttttaccc agcaactcgc tttttggttt gatcaatagt
     3901 tctgagttgg ttggcgccac tttgcggtac acaccgtcat ggtcgatgac cagcaccaca
     3961 tcctgcatgg aggcgaacag ggcgcgcaat tcaagttctg attcactttg attctccacg
     4021 gcttgtgttg gcatggcagg ctttaccatg atcaatgcag cagcgcgccc atcaaactcg
     4081 atcatgtggg tttcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttttgc
     4201 ttgtgatttt ggatgtgcgt tgcgaggcat cgaattcatc ctttgcattc gagatccatg
     4261 tttgaagctg gtgttgactt tcaaatgtta catatcttat ttgtgtgatt tctctcgtag
     4321 gtttgcaata ctatacggat ttacaaggga agtcaattat tatattaccc tctttttaca
     4381 ccctccatca tgataaaaga atggcacagt ggagagtatc ttactgtgcc ataagagcct
     4441 ataaaccgat attctttttt aagtaggacc gtaattttgg cggcctgacc gcactgacat
     4501 ccactttttt ggcgcggatc attctggcaa aacgggccag tccttttgcg agagcactgg
     4561 caaaggcctc atccttcggc gcatcatcct ccagccaaaa gccgaggatg tgaagcgttt
     4621 cggtctggcg atccagtttg gggtcaaggc gtgccacgag gtcgtcaccg tataagatcg
     4681 gcagggtgta gtatccccag cggcgctgat gcaacggttt gtacacttcc catagatatt
     4741 caaaatcaaa aaccactttg gcccgtccac gtgcgctgac catttccagc ggggccagga
     4801 atgtaacttc ttcctgggtg gatgtgtcaa gcagtttcca ttttttggga atacgccccg
     4861 cttcaagatc agacaggtgg gactgttcac tgtttaaaac gagatatcca tcctttgagc
     4921 cttccacacg cagccttgat gcaatgccct gttcgatgag ccgctcaatg cgttttttgc
     4981 cttcttcccg cgaaacatcg cgatggataa aaccagccaa acctgtgcgc cagacgtttt
     5041 cgcgcgccag accaagatgg gcaatggttt tacggctgaa gaaatcctcc gcttctgaat
     5101 cgggtacggt atgctcatat ttttttggaa ggactcgttc ggtcaggtcg tagattcggt
     5161 caaagccttt gcggttggtg atcatcactt caccgatcaa ccacaaataa aaaagcgcga
     5221 tcgattcttc cttccgaccg cgatagctcc aactcttaag cgcctcgccg ctaaaatcac
     5281 ggtttcccag cggcccgcgt gtgcgcagtt cattcaagac ggcattcaat aattttgcag
     5341 gcggttgttg ataggcatca tgataataaa taccctgttt cttgcggtgt ttcatgtgca
     5401 gccgccagta ggggaattca ctcatgggat acatgaatag ccagccgcca tagtcaaaaa
     5461 atccgcgttt ttcatacgcg gcttgataaa gattctctgg tttgaaatcg agtacgcggc
     5521 cgtacagggc aatttcctgg ctgcgtgcaa cgatgttgag cggatcgagt tgaatgcctt
     5581 ccatctgctg aatggcggag gtggtgcctg ccagcccttt aaaccgtcgg ccgggccaaa
     5641 gcccttgttg gccgagcagg tatcggcgga atgtgatgcg tgaaattgtg tccataaaaa
     5701 aataaccgag gagatgagtc ctcggtctcc atttgttatt ggatttattt agtcatcacc
     5761 cagggaggcg atggctcgca gcaggccaaa gatcagcaca ccaagtttga ggccttctcc
     5821 ggcggtaagt gcggtttcgc gttcattttt ttctgcgcgg cgggtgagga gcatggcagc
     5881 gatgagacct gtgacagcgc cgatcaatgc gccaagtaag atgtttttgc tgttgttcat
     5941 gagttgctga tccttccaaa aaaggctttt agattttcga caaggtcatt gatggcaagg
     6001 atgggttttg cggccatgtc tgcgccgcgg gtgatgtagc cgcgggcttt gtggacataa
     6061 tcctgtgcga tgccggtgta gtatggaagt ccgctcaacg cacgggccat caggtaaatg
     6121 ccgccaatga gcagggccag tacgatcaac cccgtgatca gaagaggaat gatgatccag
     6181 atggtggata tggcggccca acgccccaca tcgccgccgg atttgaaggt ggcgaatgaa
     6241 atcagaacga tcatgccgat catcaacaaa gccgaaagta caacgggcag gatgacctgt
     6301 gtgttgcgct gcttgcgatg cacgagatag gaataatgtt cagggtgtgg aagttgagct
     6361 ttcattgctt atattttagc atagtggcat ttgttttact aagcacaata aggttacggc
     6421 gtgctgtctt cgagcggtgt gcgttcgttg agcgcctgca gcgggcggtt gttgccttca
     6481 aataccgtta ataacttttc cagttgtgcg gcagatactt ccacgggtga ggtcattacg
     6541 agccagctta caccttcaga acaaggtggg gttgtaagag acccgctgta ccggaaggtg
     6601 gtctgttcgg ctggcaggaa atcggcggca ttgaccttgc tcccggcatc tttggcgtcg
     6661 gtcttttcgg caggcaggtt gttgacgaat ggatcgaagg ctgcattctc tgtgccttgt
     6721 tgaagcagaa tgccaatgac agccagtttc ccctctgcat ttttatggac gacgtggatc
     6781 tcggctgcga atgcctgccc atcaatgtgg tgttcgctgg gagcatgata atgaaattga
     6841 accacatcgt agcgggtccc atccaattca atataactgc ccgtatcgta attgacctgg
     6901 gctgtgtgcc cgttgtttag gatattcact tcactgggtt ggtagtggaa tgagatgttg
     6961 acgaggtctt tttcaatggg ttttgaaata tcaatgggtg attgattctc acctgtgccg
     7021 cagacagcat aggatgggtc gatttctccc cagtgttgcg ggccttcctc gccttcgtag
     7081 gtccaatgcg gggcttcctg cggttggttt gcacatgcgc ctaacagtaa agatgcgatg
     7141 atgagcagtt ttttcatacg ggtctccttg ataaagttat gcaatcacca aggcctatta
     7201 tccatctcat ttgcggttat tggcaatata aaaaagcaaa catgacttcc cccgaaatta
     7261 aggtttcgcg gttaagttcg tatcttaaga acaaatagga agctgattta cacacacctg
     7321 ccctggcggg tggcgccaag ggaaaacgct gatttttgct tttgatcagc gtaaatcagc
     7381 gttttaataa aactcagggt taattatgtt tgtctggtta ttgtgctggc ttgccttcga
     7441 ctggatatcc tttggcatac cattctttta acccgccagc catgctggtg gcgttgtatc
     7501 ctgctgcgag caggatgtca cgtccctgct ggctgcggtt tcccgaacgg cataccacaa
     7561 gaatttcacg gtcttttgga atttctgcca gtcggctttg cagttggtca agcggaatta
     7621 gtgtggtgtt gggtgcatgg tattcatccc attcctcttg tgtgcgaaca tccacaacaa
     7681 aggcgccgtt ttgatacatt tcatacgcct ggtctgtgtt gacctcgcgt gacaatccct
     7741 gcccgctgcc ctgcccggat aaggcgatca atacgatgac aatgaccaca actgcgatca
     7801 gagtcaactg cccggctggt ttgcgcagaa aagcggaaag tccgctgcgg gaggtggtgt
     7861 ttctattttt cttttttgac atatgccctc acaaatgatt ttgatttctt ttttaggacg
     7921 tagattttgc agatggtctt acacaaacag ggaatgagtt ttggttaaaa atgttttcct
     7981 ccttctttgg tcttaattga ccaggaagga ggagcttttt caagtatttg tttgtgctgc
     8041 gccttgtgtg acgctttgta agactgctag taagatttga ccgccgcatc aaggtcgtga
     8101 tacacatcga agaattgttt caaccccaca acatccaaca cctgctccac tgagggctgc
     8161 gggccgaaca acttcacatg ctggcggaca gcaccatcac gttcgaaatt gatcgcgcga
     8221 aaagacgggc gccccgtttc cttcgaatac atggattgac cggcgaagat cagcaccacg
     8281 gtgtgtaaag acatcaaccc tgcactgctg acatatgaaa ccccgctcaa gtcgatcaga
     8341 atatcgcgca caccgctggc gtataactcc tgagccttgt taatgacctc gatgtagttg
     8401 gacgcatcca gattgcccga aaggtgcatg atggttacaa atacgcgcca ttcctgtttt
     8461 gtgattctga tttgcatggg ttcctccggg ttgtaacggt gtttgcattt tacctgaaat
     8521 tgaattcttc aaagtggatg ttgtctgcgg ggactttcat ttcaatgaac tttgcttcga
     8581 aggcctgcac catgggaagc ggaccgcaca tgtaaacatg gtgcgtgctg atgtcaccgc
     8641 ctgcattttt ggcgatctcc tccacggtca acgagccatc tgtggaggaa aagcggatgt
     8701 gggtcttcaa ccgcggatat ttttgtgcgg cggtttgaat ctcttccacg aagatggctt
     8761 cttcacggtg tcttaccgtg tagtaaaaat ccacatcgaa ggcagggtca gttttcaggt
     8821 cccgaatgaa cgagaggaag ggggtgatgc cgattccgcc tgcgatccag atctgtttgg
     8881 ggccgccgct cttgtagtcg aacattccat atgacccttc caccgtagcg gacatgcccg
     8941 cctttaagtt cgcaaacagg tgacgggtga aatcgccgca tgccttgatg gtgacccgca
     9001 gcacatcttc gtgcggcgcg ctggagatgg tgaacggatg cgcttcgtcc aacccttcgc
     9061 ctgcgaaatg cacgaaaagg aattgccccg ccttttgatt tttgatcgat tccttttttg
     9121 cgcgcaacac cacttcggtg gtggaattgt tcgggtggtt gaccgcctct actgtgtatg
     9181 gcaggtattt tttgaagagc ccgccgaaga cctcggtgta taaataggaa agtgtgccaa
     9241 ggatgaagaa gttctgtgtc cacgtgatgg cgatttttgc gctgggggca ttgatggtca
     9301 gcgaatggac gaagccgaga ataaaaaaga tgccgacgaa acgatgtagt tttttccagc
     9361 cttcgtaggt ttggccggtc aatttgctca agaacgggat gcgtggtgcc agggtcggta
     9421 gaacgatcgc cacgatcccc agaaaagcga ccatggcaag gtagttgccc acgcgcaggt
     9481 tgacgagtgt gatgggcacg gtcagcagat gtacgaacat caacagaaat gcactggtgg
     9541 atgtgtgacg gtgggtcatg tacatcttgt ccaggccgcc aaaaaacggc tctgcccatt
     9601 tgggacgcgt ggaaatgaac aacgaaaatg acatcaacac aatgaccagg gagccgagga
     9661 cttcccctgc ataggcacgt gcgtaatgtt cagccccctc tgccacaggc ggaaaaacga
     9721 tccagatgat aagtgtgagt gccacaagga tgataatggt aaggttcccg atattttttt
     9781 tcttcatctt gcctctggtt gatctgtttt gtcatgaatc aacagcgatg cagtggaaac
     9841 tgcatcgctg gggaaagcgc aaatactaaa aagaatcgat tgctttttgc ctgtcggtga
     9901 aggtttcgaa gaaggcggaa aagccgacca tatcgagcac actgataact tcaggacgcg
     9961 gattaagaag tttgaggttc ttctgcatcc caccatcgcg gctgcggtcc atggatttga
    10021 gggtggccca gccatgttca aggtctggca attcctcgcc gcgggtcatc agtgcaatgg
    10081 tatgcaatga aaccaacccc gcgctggaaa tataagtcag gtcgccgagg tcaagcagga
    10141 tgttccttgt accagcctcc acaacttcac gcgccttggc gatgagagtt tgataggtct
    10201 gcccatccaa atgacctgcc aacttgatga cagcaacgga aatgcggcct tgctcttgtg
    10261 agatggtaat ttccatttga atactccttt caataaatga tgccgtatta taaacctgct
    10321 ttacgttcca atcgttgcag gcggtggtct atttctccga gccagtgttt gcggatgtat
    10381 ggaggcaggt cggcccgttc cacttctgcg cgtgccgacc tggcccattt gagcgaggtt
    10441 tctgcatcgc gcattttgtg ctcgtaatat tttgccagtt caatatgggc atagacatga
    10501 ccgcgctgtg cggcctgctc ccacaagcgg atggcctggt ctgcgtcacc acgttttttt
    10561 tgcaggatgg ataaccgttt cacggccacg ccgaaatctg attcgtttaa cccaagctca
    10621 agtccacgct cgaagagacg ggcggcttca tcccagtggc cgaggtcttc gaataatttg
    10681 ccgagtgcaa tgaaatcaag tccgtgttcc acacggccat tgtaggggtc tgccagcagt
    10741 tcgctgacat gtgccagcag ggcggccatg gccaccacat ccatggcgtt gtgatagaac
    10801 acgcccgcca ttgggctggc atcgcctgta cgcagatagt cgaaatataa ccagggaatt
    10861 tcataacctg gcacttcttc agaagtgcgg gtaaagccga gtacatgttc ttcaagatac
    10921 ttcaacgctc gcgaagggag tctgtcgcgc cacaaccgcc gtgccagcgg aagcaggtcg
    10981 aggtgggcat aatctttgaa agggacgggg atgcggtgaa gcgaatagcg ggtgacgagc
    11041 agcggcgcat cgaaggcttt tccattgaag gttaccagtc cttcgcaggg cgcgagaaaa
    11101 tcgatcaatg cttcgagcat ggcgggttct tcagacgggt cgcgcaggaa gaattgccgt
    11161 agaacgaacc tgccatctac aaaacgaccg gctcccacaa ggaaggcata ggtccctgtg
    11221 ccgcctgcca tccccgatgt ttcagtgtcg aggaatgcaa atttatggat cggaagctct
    11281 gccatacgcg gatcgttggc ccattgggca atgatcgaaa atggaaaaga ggaggcgtaa
    11341 gccgttttcc cgtgcagata atcttcggca aaggtttgtt cggcgatgaa tgcctcgccg
    11401 cgcggggtct ggaaaaatgt cccagccacg accgagtcaa tggaacggct ggcaggtttg
    11461 ggcgcgggaa gatccgctgt tcctgttttt acaccgagcg ctttgagttt atccgcaagc
    11521 gatgacatgg gatgtattgt atccatgact cagcactttg ccaaatcgaa ccagctttgt
    11581 atagggtgct ataatgattt aaacaaaatc aatttacccc catgaaagga tgtccctttg
    11641 gtaaaagtca actatcaata cgaaaaacgc aaaaaagaac tggataaaaa acgcaagaaa
    11701 gagcagaaat taaagctcaa acagcagaaa aaaaataacc cgtctggtga ggttttgacc
    11761 gaggtgcagg atggtgccat ggttgaagcc ccagcggaga ccatgcccgt gaccccgcct
    11821 gttgaagaag tggataaata aaaatacgag aagcaataag aagaggagat ggagttccat
    11881 ctcctcttta ttttttaaaa gttgaaaaac ataataatcc tgcaattatg agtttcttga
    11941 caaattagaa caagtgttct atattagagg cagttcgatt gttttttcat ctggttccaa
    12001 aaaaggagat ttcccatgaa ccgctctctc aatcttcaat tgaaactgct tccctttttg
    12061 aaaagcgcat ctctcaaacg cggcgcgatc tttggcggca ttctcatcag cgccctgctt
    12121 gcctttgagt tgttcaactt cggcacgaca tcctttgcct tgcaggatgt actcggtaat
    12181 ttgaaattcg cgggtattca atggtctgcc atccttgcct ttgccttttg cggaattgac
    12241 tttgccggta ttgcccgcat ttttacgcct gagcagggtc gtgacgaacc tgccgaggtc
    12301 tggtacctgt ttggggcctg gctgcttgcg gctggtttca atgccatgct tacctggtgg
    12361 ggtgtttcgg tggcgatcct caaccacaat gccgaaggcg gctctttggt ggggcaggcg
    12421 gtgatggtga aggctgtgcc gatctttgtg gctgtgatgg tctggttgat ccgtgtgttg
    12481 atcattggca cattctccat tgccggggaa cgcctcttct cgctggcgga agcaacttca
    12541 cgacctgccg ctactgcgta tcaaaataat tcgccgcgcc cggttacaca atcacaacct
    12601 gtgtatcgcc cggcagccat gagcgcgagt tccagcaatc aaaattatcc gcgccctgca
    12661 cccaaaccgc gcccggcacc ggtttcaccg aactatggcg ccaaccgtcc tgaacctacc
    12721 tatcacccca ttggtatgac tgcaatggga catggtgaag gcagcaattc cacatcttct
    12781 gtgcggagat aggacacgac tgaagtcctt taatcactga tactctctca cgcccgtttg
    12841 ggcgaaccac tgaggcgccc aaacgggcgc ctcatgcgtt aaggtgactg gtgtattttt
    12901 actcgaactc gtccagtgtg ctttcgccgt gggtgaggat gtaaagtccg cgtggggtgc
    12961 cgttgtttgg gtcgaagaca aaatcggtga cgatgaaatt tttttcgaat gcggtttcga
    13021 acaattcgcg ggtgaagaag cgccagtcgc gggcgagggc gaagtcttgt gatttgaggg
    13081 cggtaaagtc gcttggaatt tccgcgagca gcaggcggtc gtcaaagggt ggaacatgct
    13141 cgggtggttg tggcaggcca ttggtcgagg tgcgaagcgg gtagaactgg cgcaggcctg
    13201 agctggcaac gtgagataac ttgagggtgg ggcgcggccg tttgctgagt cttctttcca
    13261 cacgcggagt gttgatccac caatctactt gaaatcggtc ggagggcaga ccagcgttga
    13321 gaccgtcgcg catgtcgcca tattcagacc gccgataggt gacacagacc gctcccagtt
    13381 tggagatgtt gagataggcg ttccgactga gaagagggtc atacgtccat gtaacgtggt
    13441 cgaggtcctg atggcggacc atttgccatt gggcccgttt gagcgaaaag ccaaccccgc
    13501 catcgcgata gtcaggatga atgcccatca tgtgcgagca atgtttgggg cgcgggccgt
    13561 ccggtgtgga ttcgagaccg ggaaagccaa agacaaaccc gacaatcttt tcttccacaa
    13621 atgcgcccag caccaacccg ccgttgtgaa cggcggtgat gagcatatgc aacggcacca
    13681 cgtctgtttc ggagccgggc catacatcgc gctgcaggtc ttcaacaagg cgcatttctt
    13741 cgggagtttc gatgaggcgg atggagaaat tgttcatggt tatcgagtgc ggagcgctgc
    13801 aattctttct gtaagggtgt tccatgcgcg ttgataccac ggcttgcgga tcaggtaatc
    13861 gaggcggtag gtgaaacgcg caggcatgag gccaaatacc cgcggcatcg aatccacggc
    13921 gcaggtgcgg ttgacggcga aataatcgag ccagaaagac gagatgggaa agttggggat
    13981 gacaccatcg agaaatactg tgatggcgcg cagggtgatc ggcgaaagcg gaaccagcac
    14041 acgccgttga cgggtcacat ccatgatggt ttcaatgatc tgctgcaggg tgaaaaactc
    14101 actgccgccg agctcgtaca cgcggttgag cgtgtcttca ttttcaagcg accacaaaat
    14161 acaggtggca aggtcttcca cccatacagg ctgcaccacc gtgcgcccgc cggactgaat
    14221 gggaaacaac cccaccgaag cccgcaccag tcttgaaagg ttattcgtaa aatggtcttc
    14281 aggtccgtat accagtgacg agcgcaggat cgtatatgga accttgccat tgcggatgtg
    14341 ttcctcggcg atgccttttg ccctgaaggc gggatagccc gaagagcgcg ccgcgcctat
    14401 gtgactgaga tacacgatcc gatccacacc cgcatctgct gctgcttcga cgacgttctc
    14461 ggtgccttga atatcggctg ttaaaagatt gccgcggctg ccctgatttt ccgcactggc
    14521 gaggtgaata acggcttcca cgtcacgcat ggcggcgcgc aggccgcgct tatcggcaag
    14581 actcaccacc gccacttcca caggcacgcc tttggggagg ttcggcgtcc gcggcgaagg
    14641 gcgaatcaag gcccgtagtg gatatccaat ggaagaaagc tgtcgcacca gtgtacgtcc
    14701 gatgaaaccg gtggcgcctg tgatgagaat catggctggg attatagcag agggccgcaa
    14761 acgctgacgc gtagcgctcg aaccccagtt actcggtaaa ataaaaatac cccaaggtga
    14821 cggggtattt tgtgggcgct gacgc
//
LOCUS       DAOQJC010000101        14746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_1953_length_14746_cov_9.044789, whole genome shotgun sequence.
ACCESSION   DAOQJC010000101 DAOQJC010000000
VERSION     DAOQJC010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14746
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1953_length_14746_cov_9.044789"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="PLE39_14125"
     CDS             <1..1408
                     /locus_tag="PLE39_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69518.1"
                     /translation="LLVSVAFGGLLFFIVWVILRRQVHRAAFLTTLLLALFFTYGHAY
                     IAVDEKWPDAGYAQWLAVVWAILLVLAVFWATRPKWTFVSAAANLNTVALALVIMATA
                     QIDFSNGMGKVHRVALEKAPIQEDLTLPENPPDVYFFLLDSYGRSDLLYAAYGFDNSD
                     FENQLVDRGFYIASCSQSNYVRTEISLGSSLNMAYLQGLDETFTPDSTARRVLWDALK
                     HNAVRYNFETLGYETINFASGFAWLEITDVDHFMSPPPASSGMNEFEGLFLRTTLARY
                     AQDLGWVDPDYLMGVSFRDRFNYIFNSMDEITAMPQPTFAYMHLISPHPPFVFDPEGN
                     PTYPPDFWNDQRMYPADMYQKGYVNQLQYLNKNMLEAVDTILANSDTPPIIIIQSDHG
                     PWLQPKDKRMRNFTAILMPGHEDVLYPTITPVNIFRVVFNAYFGGKYDILEDVSYFSP
                     VPKLYDFSEIKNPCANGN"
     gene            1412..2362
                     /locus_tag="PLE39_14130"
     CDS             1412..2362
                     /locus_tag="PLE39_14130"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNE69519.1"
                     /translation="MKKTVLLTAPYMLPFLERFRPEFEKYDLDLIVPDVEERMEEADL
                     LKYAGQFDGAVCGDDRYTARVIEACSPRLKVISKWGTGVDSIDAEACSRFNVILSRTP
                     NAFTTPVADTVLGYMLAFARRGPWMDREMKNGKWEKIPGKALSELTLGIIGIGNIGKA
                     VTRRAKAFGMKVLGTDIVDVDHVFVNESGIEMTDLHSLLSNSDFVSVNCDLNPTSHHL
                     INAKTLSLMKNTAVLINTARGPIVEEPALIAALRSGQVGGAALDVFEYEPLPKDSPLL
                     KMDNVMLAPHNSNSSPTAWERIHWNTIKNLVTGLGLEYKG"
     gene            2370..>2632
                     /locus_tag="PLE39_14135"
     CDS             2370..>2632
                     /locus_tag="PLE39_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69520.1"
                     /translation="MSKLRSALPYLFIFLLAALVLDLGNPLFDKPARDGGFFLYAGSQ
                     IISGKIPYVDFWDSKGPAIFYINALGLFLGQGSRWGVWGVEFV"
     assembly_gap    2633..2732
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2733..4049
                     /locus_tag="PLE39_14140"
     CDS             <2733..4049
                     /locus_tag="PLE39_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69521.1"
                     /translation="GLRIVLGYGNYTEEYALLFNALAVYLFFVVDENKGWKYFGIGLL
                     FGISFSFRANNIGGLFAVLIALALAGFVGSLAKTPSPNHRDTESRRFNGFFPGASMSP
                     WFKSFFIRLLFVLAGFAAPLLLWVLFFQIHGAAWEMIFASIIFNFSYASAKSREWLDI
                     FGGFGRYGMLWVGWVAAFGYVVNGYHVLRKALHRNPLTLVDWFLFTWFPFEILLSNLS
                     GRNFSHYYISWALAVAVYGAALFTSGTGFSLPKSLTALTTGVRTSFLYDGMVLFFIVG
                     LLLFSTPSIKRYGETVSRSFSGQSLEYSDPLADAIRANTQQDDLVLTWYPETTLNFMA
                     GRASPVKYVYYPLFLEGTLPPGAETSYIEGLTSRRPEMIVDCSRAVDAIPSLDPAARE
                     DQFSKPGVKKKMYFQPGMEEIFNFVAENYHVETSVDGCLLFRLNLE"
     gene            4054..4815
                     /locus_tag="PLE39_14145"
     CDS             4054..4815
                     /locus_tag="PLE39_14145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE69522.1"
                     /translation="MDTQKFVLITGAAGGIGRATVNLFFEKGWHVVGIDRSDFGEGFP
                     ANGLFVRSDISRPEDMQAIFEKVSAVTDSLDALVNNAAMQVAKPLVETTVEEWDAVMN
                     ANLRSVFLGVKLAHPLLKARGGAAVVNVSSVHAIQTSANIAAYAASKGGLLALTRAMA
                     IEFAPDNIRANAILPGAVDTPMLRAGLGRGHVGQGDVQDRLDNLARKTVNGRVGTPEE
                     IAHAIYFLADNQQSSFMTGQALVVDGGATARLSTE"
     gene            4900..5304
                     /locus_tag="PLE39_14150"
     CDS             4900..5304
                     /locus_tag="PLE39_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69523.1"
                     /translation="MKRRTRKTKFLTDRAYVPASRKTLVRILFFINAALWLGYVVYIY
                     YDMAVVNKNLTSADIVSLFVFANSVMMFVSGWQLAKNESWAYYLAMVVIGINLLLTLL
                     NILDLFFLGIFLLDLFILLSLIPIRKHYLSKP"
     gene            5301..5636
                     /locus_tag="PLE39_14155"
     CDS             5301..5636
                     /locus_tag="PLE39_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69524.1"
                     /translation="MKNIKTVGSLFFVTAFVLAVFGGASFSRAHANPIYNLYAVLMFV
                     DAAVMLVCGLYINRNTQAVYWFAVMALTLNIVLTIFDQIGLVDILFAALNFVTLMALV
                     SSRKEFSHQ"
     assembly_gap    5754..5853
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5854..6603
                     /locus_tag="PLE39_14160"
     CDS             <5854..6603
                     /locus_tag="PLE39_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNE69525.1"
                     /translation="AVRRIPQLPDAYLHPWRRESIRRLAALKDIHKGQRAFIIGNGPS
                     LKQTDLTKLKNEFTFGMNRIYLLFPELGFHTTYFCSINDLVIEQFSDDILALPMPKFL
                     AWRSHRHFSKNLQLANIPTFLYTTYTGPRFTPDVRRRVWEGATVTNFALQLAFHMGFE
                     KVILIGVDHNFASKGDANKTVVSEGDDPNHFSPNYFAKGFKWQLPDLDTSEIGYTFAR
                     EAYRKAGREVLDATVGGKLTIFPKVDYNSLF"
     gene            6664..8136
                     /locus_tag="PLE39_14165"
     CDS             6664..8136
                     /locus_tag="PLE39_14165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE69526.1"
                     /translation="MTPPTRPANWFIAFIILLALGGVLRLLDLTDPPLDFQPSRQLRN
                     SLVARDIYYSMLPSATAEQKELASSFARSVGQYEPPVIESIVAVTFLVTGGENFIAAR
                     IWESLFWLAAGIALFDLMRRAVSPWAAVVAIAFYFVLPFSVQASRSFQPDPLMTAAFV
                     AGIYFLYRWSEEQTWKWAILTGVFFGFATFVKIVIAFFVGAAAISLVLFTLKKDFWKS
                     KQVWVMAAIMVVPAFLFYILLNQGRSTEYFFAWTVTLIKLITSADFYSKWLAFVGSIF
                     GLTMIFLSLAGALIATPRLRWLLVSLWVGYVLYGLTLPFQMYTHSYYHIQLIPIIALG
                     LASALNPLIESVAGQGQVGRVGVIALIVVVIGYQSWVARSVLIAEDFHHEPAFWKQVG
                     DAIPANTDVIALTQDYGYRLMLFGWRKVSLWPLSTGLSEARGGNTDVAGNFADLTAGK
                     EYFLVTAQGQFDKQPELKKILSQYPIAAQGEGYVLFDLRK"
     gene            8133..9062
                     /locus_tag="PLE39_14170"
     CDS             8133..9062
                     /locus_tag="PLE39_14170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE69527.1"
                     /translation="MTLVSIITPSYNQAAYLEQTLLSVLNQDHAPIEYIVVDGASKDA
                     SVEIIKKYSDRLAYWVSEKDGGQAEAINKGFARATGEIIAWLNSDDYYLPGAVSAAVK
                     MFEANPEMVLVYGNMLAVDEHGKTFNTLTYKQLTLEDLLCFQIIGQPAVFMRRSALQK
                     TSGLDSTFHFLLDHFLWIQLAQHGKILHADQTWAAARYHAEAKNRAKAAEFGREAFRI
                     LEAVAQDKNLAPVLSKVERRSRASAHRVNARYLLDGGQPAAALSAWVRAFFIHPPTAL
                     ARMNLFVSAILNLFGLGKLRSMVLRMREARHRE"
     gene            complement(9280..9747)
                     /locus_tag="PLE39_14175"
     CDS             complement(9280..9747)
                     /locus_tag="PLE39_14175"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNE69528.1"
                     /translation="MPYLIDGHNLIPKVGLRLDSSDDEVKLTRLLQNFARLKRETVEV
                     YFDGAPPGQAGTRKLSGITAHFVRAGQTADSAIRARLKNMGKTAKQWTVVSSDREVQS
                     AAQTARAQFTSSESFSKLVEQVINSAPPGATDERLSAAEVDEWLEIFQDKSTK"
     gene            complement(9822..10214)
                     /locus_tag="PLE39_14180"
     CDS             complement(9822..10214)
                     /locus_tag="PLE39_14180"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNE69529.1"
                     /translation="MEMLIDFPGGLRVDAHFRGHTIATDQPPADSAPMPFEVFLASIG
                     TCAGIYVLGFCRQRNLPTDGIHIIQRNHANPMNGMVSEIDLEIQVPPSFPAQYYDALV
                     RSAELCKVKKTLENPPTFNVMTKVLEPA"
     gene            10372..10734
                     /locus_tag="PLE39_14185"
     CDS             10372..10734
                     /locus_tag="PLE39_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69530.1"
                     /translation="MTEKKNTNITVSPQSGMIHNLVVRTKLVLRLLGDKRVSSWAKLI
                     PVGSMIYLLSPIDAIMGVPGLDALDDAAIVGLGYYFFIELCPPDVVQEHLQALEGTNE
                     PPQAREDVIDGEATEVKE"
     gene            10747..11973
                     /locus_tag="PLE39_14190"
     CDS             10747..11973
                     /locus_tag="PLE39_14190"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNE69531.1"
                     /translation="MILLTLPILACGGLAESVVATITPQAAPTIAAPPQTESPPTVDS
                     ATEPPVISPTEVTAANVNTFPNTDNYQWTLIASGLSRPVDIQSAHDGTGRLFIVEKYG
                     VIRVYENGQLLDQPFLDISDRVGDESNEQGLLGLAFVPNNSGVLFVNYTDNNGNTVIS
                     RVLWDQVANPLSEKDLLHVEQPYPNHNGGALAIGPDGYLYIALGDGGLAGDPHKNGQN
                     TNSLLGKILRIDISDLNNPNNYSIPADNPFGNEVWAYGLRNPWRISFDRVTGDLWIGD
                     VGQGDWEEIDYLPAGSAGGANFGWSVMEGSYGYDGVAQPGMLLPAAEYSHNEGGCSVT
                     GGYVYRGAMTEWNGIYLYGDYCTGKIWGLILSNGQWQSQVLFEAGETITTFGQDEFGE
                     IYFASDSGNVYRLTKK"
     gene            12085..12303
                     /locus_tag="PLE39_14195"
     CDS             12085..12303
                     /locus_tag="PLE39_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69532.1"
                     /translation="MAEKIWQVEKIKYCEHVGHEVAIENEVVYPAEHLPEQLPRIVAH
                     RCSNAIECNLIDKATCALCGTNPDLDPV"
     gene            complement(12412..13146)
                     /locus_tag="PLE39_14200"
     CDS             complement(12412..13146)
                     /locus_tag="PLE39_14200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE69533.1"
                     /translation="MSETKTTNLIEIRALRVQRNQRDALHVASLDIPRGETLAIVGPN
                     GSGKSTLLLTLAHLLRPASGDVKYGGKSLREWNDLEYRRKIAFVFQDPLLMDMSVEEN
                     VALGLKFRGMDKPESQMRSEKWMRSMGVDSLSKRRAGQLSGGEAQRVSLARAFVLDPE
                     LLLMDEPFSAVDPQTRAQLLQDLSTLLSHDHRTTIIVTHNLKEAAQMGDRVAVIINGE
                     LKQVGTPQEVKNSPADESVERFLKEL"
     gene            complement(13143..13787)
                     /locus_tag="PLE39_14205"
     CDS             complement(13143..13787)
                     /locus_tag="PLE39_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE69534.1"
                     /translation="MSEIFEITALSLQVSTIATFVSLLIGLPLGTWLALGNFRGRAFI
                     LSIVNTGMALPPVVVGLVVAMTLWRSGPLGSLRLIYTPWAIVIAQTIISAPVVIGLTA
                     AALAALDPRLQQQLLGLGASRLQMIWHLWREARLPLLAALMAGFGSVISEVGASMMVG
                     GNIRGQTRVLTTAIVLETSKGEFERALALSALLLIITYLINLALTWVQQKGQRR"
     gene            complement(13874..>14746)
                     /locus_tag="PLE39_14210"
     CDS             complement(13874..>14746)
                     /locus_tag="PLE39_14210"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNE69535.1"
                     /translation="TAAPATEAPVATEAPATEAPTEAPTEAPAAAPANPNLILATTTS
                     TQDSGLLDVLVPMFEEQTGYTVQTVAVGTGEALKMGEEGNADVLLVHAPSSEVTFMDG
                     GFGKDRMLVMHNDYIIVGPAADPAGIKGLGPKDAFVAIYNAGAPFVSRGDDSGTHKKE
                     VSFWTKAELDPRTEKPAWFIETGQGMGASLTVASEKGAYILTDRATYLSNKANLQLEI
                     LLEGNNALLNVYHVITVNPDKWTAVNYDGAMAFAKFITDPATQAVIAEFGKEKFGQPL
                     FYPDADKTDADLGL"
BASE COUNT         3176 a         3659 c         3868 g         3843 t
ORIGIN      
        1 gctgttggtt tctgtggcgt tcggcggttt gttgtttttt atcgtctggg tgattctgcg
       61 caggcaggtg catcgcgccg cgtttttgac cactctgctg ctggccctgt tctttaccta
      121 cggccatgcc tacattgccg tggatgaaaa atggcccgat gctgggtatg cccaatggct
      181 ggccgtggtt tgggcgatcc ttttggttct ggctgtcttt tgggccaccc gcccaaagtg
      241 gacctttgtc tctgcggcgg cgaatttgaa cacggttgct ctggctttgg tcatcatggc
      301 tacggcgcaa attgattttt cgaatggcat gggcaaggtt catcgggtgg ctttggagaa
      361 ggctcccatt caggaagacc tgaccctgcc cgagaatccg cctgatgtgt atttcttttt
      421 gctcgattcc tacggccgct ctgacttgtt gtacgccgcc tatgggtttg ataacagcga
      481 ttttgaaaac caactggtgg accgcggatt ttatatcgcc tcgtgcagtc agagcaatta
      541 cgtccgcact gagatttcgc tggggtcctc gcttaacatg gcatatttgc agggattgga
      601 tgaaaccttt acccccgata gcacagcgcg ccgcgtgttg tgggatgcgc tcaaacacaa
      661 tgcggttcgc tacaactttg aaacccttgg gtacgaaacc attaactttg catctggctt
      721 tgcctggttg gagatcaccg atgtggacca ttttatgagt ccgccgcctg cttcttcggg
      781 gatgaatgaa ttcgaaggct tgttcctgcg aaccactctt gcacgatacg cacaggacct
      841 cggctgggtg gaccccgatt acctgatggg cgtgagcttc cgcgaccgat ttaactatat
      901 cttcaacagc atggatgaga tcacggccat gccgcagccc acctttgcat acatgcacct
      961 gatctcgccg catccgccgt tcgtgttcga ccccgaaggt aaccccacct atccgcccga
     1021 tttttggaac gaccagcgca tgtatcccgc cgatatgtac cagaagggat atgtgaatca
     1081 attgcaatat ctcaacaaga acatgctcga agccgtggat accatccttg cgaattccga
     1141 tacgccgccg atcatcatca ttcaaagcga tcatggtccg tggttgcagc ccaaagacaa
     1201 acgcatgcgc aacttcacag ccattctcat gccaggtcat gaagatgtgc tttatcccac
     1261 catcactccc gtcaatattt tccgcgtggt cttcaatgcc tacttcggcg ggaaatatga
     1321 tattctcgaa gatgtcagtt atttttcgcc cgtgccaaag ttgtacgatt tcagcgaaat
     1381 aaaaaaccca tgcgcaaatg gaaattgatg aatgaaaaaa acagtccttc tcaccgcccc
     1441 ttacatgctt ccgtttctcg aacgttttcg acccgaattt gagaagtatg atcttgacct
     1501 gatcgtaccc gatgtggaag aaagaatgga agaagccgac ctgctcaaat atgcaggcca
     1561 gtttgacggc gccgtttgcg gcgatgaccg ttacacagcg cgcgtcatcg aagcctgttc
     1621 cccgcgtttg aaagtgatct ccaaatgggg gactggggtc gattccatcg acgccgaagc
     1681 ctgctcccgt ttcaatgtca ttctctcccg aacgccgaat gccttcacca cccccgtagc
     1741 cgatacggtt ctggggtaca tgctggcctt tgcccgcagg ggcccgtgga tggacaggga
     1801 aatgaaaaat ggcaagtggg aaaagattcc aggcaaggcg ctgagcgagc ttacactggg
     1861 aattatcggc attgggaata tcggcaaggc ggtcacccgc cgcgccaaag ccttcggtat
     1921 gaaagtcctc ggcaccgaca ttgtggatgt tgaccatgtc ttcgtcaatg aatcggggat
     1981 cgaaatgacc gacctccact ctctgctttc caattctgat tttgtttcgg tcaactgtga
     2041 tctcaacccc acatctcatc acctcatcaa tgcaaaaaca ctttcgctga tgaagaacac
     2101 agccgttctt atcaacaccg cccgcggtcc catcgtggag gagcctgccc ttatcgcagc
     2161 actccgttca ggtcaggttg gaggcgcggc gctggatgtc ttcgagtatg agccgctgcc
     2221 caaggatagt ccgctgttga agatggataa tgtcatgctt gcgccgcaca attccaactc
     2281 aagccccacg gcctgggagc ggatccattg gaacacgatc aaaaatctgg tgacagggct
     2341 gggactcgaa tacaagggct gagttcttca tgtccaagct ccgctctgcg cttccgtatc
     2401 tttttatctt tctgctggcc gcgttggttc tcgatctggg gaatccgctt tttgacaaac
     2461 cagccaggga tggtggtttt tttctgtatg cgggtagcca gataataagc gggaaaattc
     2521 cctacgttga tttttgggat tcaaaaggcc cagccatttt ttatatcaat gcgttgggct
     2581 tgtttctggg gcagggttct cgttggggtg tgtggggcgt ggagtttgtc ttnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggcctgcg gatcgtgctt ggctacggaa
     2761 attacaccga ggaatatgcc ctgctgttca acgcgctggc tgtgtattta ttttttgttg
     2821 tggatgaaaa caagggttgg aaatattttg ggatcgggtt gctgttcggc atcagctttt
     2881 cttttcgtgc caataatatc ggcgggttgt ttgcggtttt gattgccctt gcgcttgcgg
     2941 ggtttgttgg aagtcttgca aaaaccccga gccctaacca cagagacact gagtcgcgga
     3001 gatttaatgg ctttttcccc ggtgcctcca tgtctccgtg gttcaaaagt ttttttatac
     3061 gacttctatt cgttcttgct ggtttcgccg ctccgctttt gctgtgggtt ttgtttttcc
     3121 agatccatgg cgcggcatgg gagatgatct tcgcttcgat cattttcaat ttttcctatg
     3181 cctcggccaa aagcagggaa tggttggata tcttcggcgg tttcggtcgg tatggcatgt
     3241 tgtgggtggg gtgggtggct gcttttgggt atgtggtaaa tggatatcac gtgcttcgca
     3301 aggcgcttca tcgcaatccg ctcacgctgg tggattggtt cctgttcaca tggttcccgt
     3361 tcgaaattct tttgagcaat ctttcaggca ggaatttttc gcattactat attagttggg
     3421 cgctggcggt tgcggtctat ggtgcggcac tttttacaag cggcacaggc tttagtctgc
     3481 caaaatcact gaccgccctc actacgggtg tccgtacatc ttttttgtat gacgggatgg
     3541 tattgttttt tatcgtgggg ttgctgctgt tttccactcc gtctataaaa agatatggtg
     3601 agacggtttc aagatcgttt tcagggcaaa gccttgaata ttctgatccg cttgcggatg
     3661 cgattcgggc gaacacacag caggacgacc tcgtattaac gtggtatcct gaaacgactc
     3721 tgaattttat ggctggacgt gcctcgcctg tgaaatatgt ctactatccg ctgtttcttg
     3781 aggggacact cccgcccggg gctgagacaa gttacatcga aggcctgacc agtcgtcggc
     3841 cagaaatgat cgtggattgt tcgcgggcgg tggatgcgat tccgtcgctg gaccctgctg
     3901 cgcgggagga tcaattctcg aagccgggag tcaaaaagaa gatgtatttt cagcctggca
     3961 tggaagagat tttcaatttc gtggctgaga attaccacgt ggaaacttcc gtggatggtt
     4021 gtttactctt tcgattgaat ttggaataaa aaaatggaca cacaaaaatt cgttctcatt
     4081 accggcgcgg caggtggaat tggccgtgca actgtcaatc tgttctttga aaaaggctgg
     4141 catgtggtcg gcattgaccg atcggatttc ggcgaaggtt ttcctgccaa tggccttttc
     4201 gtgcgttcgg atatctcccg cccggaggat atgcaggcca tttttgaaaa ggtcagcgct
     4261 gtgacggatt cgcttgatgc gctggtgaac aatgccgcga tgcaggttgc gaagccgctg
     4321 gtggagacca ccgttgaaga gtgggatgca gtgatgaatg caaacctgcg ctcggttttt
     4381 ctcggcgtta aacttgcgca tccgttgttg aaggcgcgcg gtggggcggc ggtggtgaat
     4441 gtttcctcgg tccatgcgat tcagacttcg gccaatattg ccgcctatgc agcttcgaag
     4501 ggcggcttgc tcgcgttgac tcgtgcaatg gcgatcgaat ttgcgcccga taatatccga
     4561 gccaatgcca ttctgcctgg tgcggtggat acgcccatgc tgcgggctgg cctcgggcgc
     4621 gggcatgtcg gtcagggtga tgtgcaggac aggcttgata acctcgcccg caaaacggtc
     4681 aacgggcggg tgggtacgcc tgaagagatc gcccatgcca tttacttcct cgcggataac
     4741 cagcagtcgt cttttatgac aggtcaggca ttggtggtgg atggcggcgc aacagcgaga
     4801 ttgagcacgg aataaaaagc aactgtgcaa aggcagtgaa cgcacgaaga ttccttcttc
     4861 tttcctcttc gagcctttgc aaaaacaggt atcgcaccga tgaaaagacg aacacgaaaa
     4921 acaaaattcc tgaccgatcg cgcctatgtt cccgcatcgc gaaaaacgct tgtgcggatt
     4981 ctgttcttta tcaatgcggc gctctggctt ggttatgtgg tttacattta ttatgatatg
     5041 gcagtcgtca acaagaacct gacctcggcg gatatcgtgt cactttttgt ctttgccaat
     5101 tcggtgatga tgttcgtcag cgggtggcag cttgcaaaga acgaaagttg ggcctattat
     5161 cttgccatgg tcgtgattgg cattaatctg cttttgaccc tgctgaacat tctggatctg
     5221 ttttttctcg gcatattttt gctcgacctg ttcattctct tgagcctgat ccccattcgc
     5281 aaacactatc tttcgaagcc atgaaaaata taaaaactgt gggttctctg ttttttgtca
     5341 ctgcctttgt gctggctgtt tttggcgggg cttcgttttc acgtgcccat gccaatccca
     5401 tttacaattt gtatgcggtt ttgatgtttg tcgatgccgc tgtcatgctg gtttgtggtt
     5461 tgtacatcaa tagaaatact caggccgtgt actggtttgc agtgatggcc ctgactctaa
     5521 atatcgtttt gaccatcttc gatcagatcg gtcttgttga cattcttttt gcagcgttga
     5581 attttgtcac ccttatggcg ctggtctcat ctcgcaagga attttctcat caatgaaaca
     5641 gacgataaaa tcgcttttcc ctgaatcgac tttcaatgtc gtgcgcgacg cctacgatgc
     5701 cgtccgccgc atcccgcaat tgcccgacgc gtacctgcat ccgtggcggc gcgnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccgtcc gccgcatccc gcaattgccc
     5881 gacgcgtacc tgcatccgtg gcggcgcgaa agcatccgcc gcctggccgc gctcaaggat
     5941 attcacaaag gccagcgcgc cttcatcatt ggcaacggtc ccagcctgaa acagaccgac
     6001 ctcaccaaat tgaagaacga attcaccttc ggtatgaatc gcatttatct gctgttcccc
     6061 gagctcggat tccacaccac gtatttttgc tccatcaacg acctggtcat tgagcaattc
     6121 agcgacgata tcctcgcgct gcccatgccg aaatttcttg catggcgttc gcatcgtcac
     6181 ttttccaaaa acctgcaact tgcaaacatt ccaacgtttc tttacaccac gtacacaggt
     6241 ccgcgtttta cccctgatgt gcgcagacgc gtgtgggagg gcgcaaccgt caccaatttt
     6301 gcgctgcaac tggcttttca catgggtttc gaaaaggtca ttctcatcgg cgtggatcac
     6361 aacttcgcct cgaaaggtga cgcgaacaaa accgtcgttt ctgagggcga tgacccgaat
     6421 catttttcgc cgaactactt cgccaaaggt ttcaaatggc agcttcccga tctcgacacc
     6481 tccgaaatcg gctacacctt tgcgcgtgaa gcctatcgaa aagcaggccg cgaagtgctg
     6541 gatgcgaccg tcggcggcaa actgaccatc ttccccaaag tggattacaa ctccttgttt
     6601 tgatttgcga ttgatcccct ccatgaaatt catcctgcat cattcaccct tcctcctttg
     6661 aaaatgacgc ctcccacccg acccgcaaac tggttcatcg cctttatcat tctcctcgcc
     6721 ttgggcggag tcctccgcct cctcgacctc accgatccgc cgctggattt tcagccctca
     6781 cgtcagctcc gcaattcact ggtggcgcgg gatatttatt attccatgct gccatctgcc
     6841 actgcagagc aaaaagaatt ggcatcttcc ttcgcccgct ctgttgggca gtacgaaccg
     6901 cccgtgatcg aatccattgt ggcggtgacg tttctcgtca ctggcggaga gaatttcatc
     6961 gctgcacgta tctgggagag tctcttctgg ctggcggcgg gcattgcctt atttgacttg
     7021 atgcgtcgtg cggtttctcc gtgggcggcg gtggttgcga ttgcttttta ttttgtcctg
     7081 ccgttttcgg tgcaagccag ccgctccttt caacccgatc cgctcatgac ggcggcattt
     7141 gtggcaggaa tctacttcct ctatcgctgg tccgaagaac agacttggaa gtgggccatc
     7201 ctcacaggtg ttttctttgg atttgccaca ttcgtcaaaa ttgtcatcgc tttttttgtg
     7261 ggtgctgctg cgatttcact ggttttattc accctcaaaa aggatttttg gaagtcgaag
     7321 caagtctggg tgatggctgc gatcatggtt gtgccagcct tcctttttta cattttgctt
     7381 aatcagggcc gctctactga atactttttc gcatggacgg tcacgctcat caaactcatc
     7441 accagtgcag atttttattc caagtggctg gcgtttgtcg gcagcatctt tgggttgacg
     7501 atgatctttc tcagcctggc gggggcgttg atcgcaactc cgcgtttgcg gtggttgttg
     7561 gtcagcttgt gggtggggta tgtgctttac gggttgaccc tgccgttcca gatgtacacg
     7621 cacagctatt atcatattca actgattccc atcattgcgc ttggtttggc gtcggcgctg
     7681 aatccgctga ttgaaagcgt ggcaggtcaa ggtcaggtgg gacgcgtggg tgtcatcgcg
     7741 ttgatcgtcg ttgtgatcgg gtatcaatcc tgggtggcgc gttcagtttt gatcgcagaa
     7801 gatttccatc atgaacctgc tttttggaaa caggttggcg atgcgatccc tgcaaacacc
     7861 gatgtcattg cactcacgca ggattatggc tatcgtttga tgctgtttgg ctggcgcaag
     7921 gtgagcctgt ggccgttgag tacgggcttg agcgaggcgc gcggcggaaa caccgatgtg
     7981 gctggcaact tcgcagacct cactgctggc aaggaatatt ttcttgtgac tgcgcaaggg
     8041 caatttgata aacagcccga gttgaaaaag attttgtcgc aatatcccat tgccgcgcag
     8101 ggtgaggggt atgtgttgtt tgatttgcga aaatgaccct cgtttccatc atcactcctt
     8161 cctacaatca agccgcttac cttgagcaga cgcttctttc cgtgctgaat caggatcatg
     8221 ctccgattga atacatcgtc gttgatggcg cttcgaaaga tgcaagcgtg gagatcatca
     8281 aaaaatattc cgaccgattg gcgtattggg tttcagaaaa agacggtggg caggcggagg
     8341 cgatcaacaa agggtttgca cgtgcaacgg gtgagatcat cgcctggttg aattcagatg
     8401 attattattt acccggggca gtgagcgcgg cggtgaaaat gtttgaagcc aaccccgaga
     8461 tggtgctggt ttatggaaat atgcttgctg tggatgagca cgggaaaacc ttcaatacgc
     8521 tgacgtacaa gcaattgacc ttggaagacc tgctttgctt tcagatcatc ggtcagcctg
     8581 ccgttttcat gcgccgctct gccctgcaaa aaacgagtgg acttgattcc actttccatt
     8641 ttctactcga tcattttctc tggatccaac ttgcccagca tggaaaaatc ttacacgccg
     8701 atcaaacctg ggctgcggcg cgttatcatg ccgaagcaaa gaaccgtgcc aaagctgctg
     8761 agtttgggcg cgaagccttt cgtattttgg aagccgtcgc acaggacaag aatcttgcgc
     8821 ctgtcctttc aaaagtggag cgtcgttccc gtgcctcggc acatcgcgtg aacgcacgct
     8881 atctactgga tggcggtcag cctgccgccg cactgtccgc gtgggtacgc gcttttttca
     8941 tccacccgcc aacggccttg gcacggatga atcttttcgt ctctgcgatt ttgaatttgt
     9001 tcggtttggg gaagttgcgc tcgatggttc tgcgaatgcg cgaggcacgg cacagagagt
     9061 agaaactact gacaagaaaa tcggtgacag atgatcatct gtcaccgatt tgtcctttaa
     9121 agggtcccgc cgctcatcag catggggggg acatgcaaat caagcggcgg gggacaccta
     9181 gataccagta aaagggaggg caggtgtcga tggctagaat atacacccga attgtccaaa
     9241 taacattaat ctaagattag aaagtggagc gactttgtcc tatttagtac ttttgtcctg
     9301 gaagatttcg agccactcat ccacttcagc ggctgaaagg cgttcatccg tcgcgcctgg
     9361 cggggcggag ttgataacct gttccaccaa tttggaaaaa gattcagatg aggtaaattg
     9421 ggcccgggcg gtttgtgcgg cactttgcac ttctctgtca gaggagacca cggtccattg
     9481 ttttgcggtt ttccccatgt ttttcaaacg ggcacggatg gcgctatcgg ccgtttgacc
     9541 ggctcggaca aaatgcgctg tgattccgct gagcttgcga gttcccgcct gcccgggtgg
     9601 ggcgccatcg aaatacactt ccacggtttc gcgtttcagg cgtgcaaagt tttgcagcag
     9661 acgtgtcaac tttacttcat cgtcagatga atcgagtctt aatcccactt ttggaatcag
     9721 gttatgtcca tcaatcaaat agggcatagc gtgaatttta cccttgaatc aaggcgggca
     9781 cgggagtacc gtgcccgcat cctttgtgct gtggtttggc attaagccgg ttcaagcact
     9841 ttggtcatta cattgaacgt gggtgggttt tccagggtct tttttacctt gcataattcc
     9901 gccgagcgta cgagcgcatc gtaatattgc gcagggaagg aaggggggac ctgaatttca
     9961 agatcaattt cgctcaccat cccattcatt gggttggcgt ggttgcgctg aatgatgtga
    10021 atcccatcgg tgggcagatt gcgctggcgg caaaagccga gtacataaat ccctgcacag
    10081 gtcccgatcg aagcaagaaa cacctcaaat ggcattggcg cagaatcggc gggcggctgg
    10141 tctgttgcta tggtatggcc tcgaaaatga gcgtccactc gtaaaccgcc gggaaaatca
    10201 attaacatct ccatggggag cctccaaagt agttagtttt tgtcacaaat aattgacaac
    10261 ccgcgcacaa aaaatattac ctctttttag accaaaatcc gtatatcaag tgtcctcaaa
    10321 atccacacag cctgaggatg tataattgca ctactcagaa aaggagacac aatgacagaa
    10381 aagaaaaaca ccaacattac agtttctccg caaagcggaa tgattcacaa ccttgttgtg
    10441 cgtacgaaac ttgttttacg cttgcttggt gataaacgcg taagttcatg ggcaaaactt
    10501 atcccggttg gttcgatgat ctaccttctt tcgcccatcg acgcgatcat gggagtcccg
    10561 ggtttggacg cattggatga cgccgccatc gttggcttgg gatactactt tttcattgaa
    10621 ctgtgtccgc ccgatgtcgt tcaagagcat cttcaagcgc ttgagggcac caacgaaccg
    10681 ccccaagccc gcgaagatgt gattgatggt gaagctactg aagtaaaaga ataaatgaga
    10741 cgatttttga tcctattaac tctgccaatc cttgcctgtg ggggattggc agagtctgtt
    10801 gtagcgacga ttacgcctca agccgcgccg acaattgctg cgcccccaca aacagaatct
    10861 ccccctaccg ttgactctgc cactgagcct ccagtcattt cacctactga agtgaccgcg
    10921 gccaatgtca atacttttcc caatactgac aactatcaat ggaccctcat cgccagtggt
    10981 ctcagccgcc cagtcgatat ccaatcggct catgatggta cgggcaggct gttcattgtc
    11041 gaaaaatatg gtgtcatccg tgtatatgaa aacgggcaac tgcttgatca gccttttctg
    11101 gatatttccg accgcgtagg cgatgagtcg aatgagcagg ggctgttggg tcttgctttt
    11161 gtgccgaata actctggggt attgttcgtc aactataccg ataataacgg caacacggtc
    11221 atctctcgtg ttttgtggga tcaagtggca aacccgttga gcgaaaaaga tctcctgcat
    11281 gtcgaacagc catacccaaa tcacaatggc ggggcgttgg cgatcggccc cgatggctat
    11341 ctctatatcg cattgggcga tggcgggctg gcgggcgatc cgcacaagaa tggacagaac
    11401 accaattccc tgcttgggaa gatcttgcga atcgatatca gcgacctgaa taaccccaat
    11461 aactacagca ttcctgcgga taaccctttt ggcaatgagg tttgggcata cggcttgcgc
    11521 aatccctggc gtatttcctt tgaccgtgtc actggcgatc tatggatcgg cgatgtgggg
    11581 cagggcgatt gggaggaaat tgattatctc cccgctgggt cggcgggtgg cgcaaatttc
    11641 ggctggtctg taatggaagg cagttacgga tacgatgggg tggctcagcc tggcatgttg
    11701 ctccccgcgg cggaatatag ccataacgaa ggcgggtgct ctgttacggg cgggtatgtc
    11761 tatcgcggcg ccatgaccga gtggaatggc atttatctgt atggcgatta ttgcacaggg
    11821 aagatctggg ggctgattct gtccaatggt cagtggcagt ctcaggtcct gtttgaagcg
    11881 ggtgaaacca tcaccacttt tggacaggat gaatttggcg aaatctactt cgcaagtgat
    11941 tcgggcaatg tctatcgcct tacaaaaaaa taaggacata tcacatccac aataggtgtt
    12001 ttgtcatggg agtttgtgac gcctgtcact ggcatggcag gcgtttttgt tttaaactgg
    12061 gttcatcaga gaacggaggc agatatggca gaaaagattt ggcaagttga aaaaatcaaa
    12121 tactgcgaac atgttgggca cgaagttgcg atcgagaacg aagttgtgta cccagctgaa
    12181 catctgcctg aacagttgcc gcgcattgta gcccaccgct gctcaaacgc gatcgaatgt
    12241 aacctgatcg ataaggcaac ctgcgccctg tgtggcacga atcctgatct tgaccctgtg
    12301 taattgagca atcgcaaatt gctcttccaa actctctcct ccctctgagt tggtctattg
    12361 gctgcaccgc gtgagaacgc ggtgtatgcc gttaaaaagg ggatgacatt tctacagttc
    12421 tttcaaaaac ctctccacgc tttcatcagc aggggagttt tttacttcct gtggtgttcc
    12481 cacctgtttc aattcgccat tgatgatcac agccacgcgg tcgcccattt gtgcggcttc
    12541 tttgaggttg tgcgtaacaa tgatggtggt gcgatggtcg tgtgacaaca gtgtggaaag
    12601 gtcctgtaat aactgtgccc gtgtttgtgg atcaaccgcc gagaacggtt cgtccatcaa
    12661 cagcaattca ggatcgagga caaaagcccg tgccagactc accctctgcg cttcaccccc
    12721 tgacaattgg cctgcccgtc gcttcgacag agaatccacc cccatggatc tcatccattt
    12781 ttctgaacgc atctgcgatt caggtttatc catgccgcgg aatttcaaac caagagccac
    12841 gttttcctcc accgacatat ccatcaaaag cggatcttgg aaaacaaaag cgatctttct
    12901 gcgatattcc aaatcattcc attcgcgcag ggatttgccg ccgtatttca catcgccgct
    12961 ggcgggtctc agtaaatgtg cgagggtcaa caacaacgta cttttcccag aaccattggg
    13021 gccaacgatc gccagtgttt cgccgcgcgg aatatccaat gacgcaacat ggagtgcatc
    13081 gcgttggttg cgctgaactc gcagggctct gatctcaatg agatttgtcg tttttgtttc
    13141 gctcatcgtc tctgcccctt ttgttgtacc caggtcaggg caaggttgat taggtaggtg
    13201 atgatgagta acaaggcgct caatgccagt gcgcgctcga attccccttt gctggtttca
    13261 aggacaatgg cggttgtcag cacccgagtc tgcccgcgga tatttccgcc gaccatcatg
    13321 gatgcgccga cttctgaaat gacagaaccg aaacccgcca tgagcgcggc caaaagcggc
    13381 aaacgtgcct cccgccaaag atgccagatc atttgcaggc gtgaggctcc caaccccaaa
    13441 agttgttgct gcaaacgcgg atcaagcgcc gccagtgcag ccgccgtcaa cccaatgacc
    13501 acaggtgcgg aaatgatcgt ttgggcgatg acaatggccc acggtgtgta aatgagccgc
    13561 aggcttccca acgggccgct gcgccagagg gtcatcgcca caacaaggcc caccacaaca
    13621 ggcggtaatg ccatgcccgt gttgacaatg ctgaggatga aagcccgacc gcgaaaattg
    13681 ccgagcgcca gccatgtacc caacggcagg ccgatcagca ggctgacgaa cgtggcaatg
    13741 gttgagactt gcagagagag ggcggtgatt tcgaagattt cagacatggg tgaggggcgg
    13801 gtaaacaagt agacaggtaa acaggatttt gtgcccgttt acctgtctac tttatttatt
    13861 tagcgattgc aaattatagc cccaaatcgg catcagtttt atctgcatcg gggtagaaaa
    13921 gcggctggcc gaatttttcc ttgccgaact cggcaataac cgcctgtgtg gcggggtcgg
    13981 tgatgaattt ggcaaaggcc atggcaccgt catagttgac cgccgtccat ttatcggggt
    14041 tgaccgtgat gacgtggtaa acgttcaaca gggcattgtt gccttcgagc aaaatttcaa
    14101 gttggaggtt ggccttgttg gagaggtagg tggcgcggtc agtaaggatg tatgcaccct
    14161 tttcggaagc gaccgtaagc gaagcgccca tgccttgacc tgtttcgatg aaccaggcag
    14221 gcttttcagt gcgtggatcg agttcggctt tggtccagaa actaacttcc tttttgtgtg
    14281 tgccagagtc atcgccgcgg gagacaaaag gcgccccagc gttgtagatg gcaacaaaag
    14341 catccttggg gccgaggcct ttgattccag cagggtcggc ggcggggccg acaatgatgt
    14401 aatcattgtg catgacgagc atgcgatctt tgccgaagcc gccatccatg aaagttactt
    14461 cggaggaggg ggcatggacg agcagtacat cggcattgcc ttcttcgccc atcttgaggg
    14521 cttcacctgt gccgactgca acagtctgta cggtgtagcc ggtctgctct tcaaacatgg
    14581 gtacgagcac atccagcaac cctgaatcct gagtggaggt ggtggtagct agaatcaggt
    14641 tggggttggc gggtgcggca gcaggagctt cggtaggtgc ttcggttggg gcctcggtgg
    14701 ctggtgcctc agttgcaaca ggtgcttcag tggcaggggc agccgt
//
LOCUS       DAOQJC010000102        14391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2025_length_14391_cov_9.574009, whole genome shotgun sequence.
ACCESSION   DAOQJC010000102 DAOQJC010000000
VERSION     DAOQJC010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14391
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2025_length_14391_cov_9.574009"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1761)
                     /locus_tag="PLE39_14215"
     CDS             complement(130..1761)
                     /locus_tag="PLE39_14215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNE69536.1"
                     /translation="MIQLQLSNITLVLGAKRIFENLNWEIQNGQKIGLIGANGAGKSS
                     LFKLIEGEHSPELGGSITRARLITTGYLSQHPELDLTLTAFEAALAGNPRVAEVQADL
                     ERVEASLGDAEVYGNERRLQQALELQHKLIEEYHALGGDSYPARVRSMLLGLGLAESE
                     LNKSLSILSGGQKKLVGLARLLLVRPDILLLDEPDNHLDLPGKMFLEKLIREYDGTVV
                     LISHDRYLLDAAVTHIAELEDGKLTVFEGDYSSFIADKETRMARQEELFRAQQHEITR
                     MQIAIKRFAIWGKIYDNEKFAAKAKTMQKRLDKMDKIEKPVLDRKRMELRLNGWRGSN
                     KVLELAGVSKSFGSKQVFANLNETIWHGERVGLIGPNGAGKSVLLRMILGKETPDVGE
                     IKIGPSISIGHYAQEHETLDFNQTVVEAVRYAGEMSESRATAFLLRYLFTYKQVSQKI
                     GELSGGERSRLQLALVVLSGANFLLLDEPTNNLDIASAEVLEQALEDFEGTVLVISHD
                     RYFLDRTVERLLVIEDGQLGGYQGGYSDYLGIKGK"
     gene            1960..2781
                     /locus_tag="PLE39_14220"
     CDS             1960..2781
                     /locus_tag="PLE39_14220"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNE69537.1"
                     /translation="MPDQLNTLSQSSLQDYYDCPRRFELRHLQHLVYPAVETEPALEN
                     EKHQQEGEYFHRLVQQHLIGIPAEQVGRLANTPNLQRWWENYLSAKDLASLRNHSKLY
                     AETTLSAPLGNLRLVAKYDLIAVSSEQVLIYDWKTYRKRPKNDWLSIRWQTRVYRALL
                     VQAGAHLNGGKPFAPEQIEMIYWFADFPNEPARFTYKAEQFKRDWDALVKISDEIKTA
                     SDFPKTDDASKCSYCPYRSYCNRGIRAGDAADAELETEAEELFDINFEQIGEIAF"
     gene            2949..3020
                     /locus_tag="PLE39_14225"
     tRNA            2949..3020
                     /locus_tag="PLE39_14225"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2982..2984,aa:Arg,seq:ccg)
     gene            3088..4170
                     /locus_tag="PLE39_14230"
     CDS             3088..4170
                     /locus_tag="PLE39_14230"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE69538.1"
                     /translation="MALRNILFIIFVMLVCTGCGVKLTGEGASTAAPDFVTSTLPPSA
                     PPLATQTPIAQQSIAPTAMATHLPTEGITNTQVNIRAATSTASTSFGVIGAFETVQVL
                     GKDASGSWYQIMYAGSPNGNGWVRAEYVQVNDAGKILPVEGLTGNGGLVLEAVNLRSG
                     PGSDFESLGVLSSKDAVLVTGKDPSGAWVQVEVTGAENLKGWVTVKFLQVENLEAIPV
                     IGEIASPTSLPAEEVSTAIPPASQDGDSLQSPLTVVRFSSTESRAMQIQGNVSAPAGD
                     TEDWIQFSTGGNGVVIEALCSSDTLTIELWFDGQRTDGFSQICGEKQFVTVEPNGKYV
                     LRILETSTNQFRNTDFILTIEDIHQP"
     gene            4167..5027
                     /locus_tag="PLE39_14235"
     CDS             4167..5027
                     /locus_tag="PLE39_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69539.1"
                     /translation="MKSLPSIDTMGARPDIIRNKRIFGMIYGIMAGLAFAGSSWGWDG
                     YTLSQFHGYFPWNVLVTGALLCAIVGGFIGWLTARMESSLAGVLFWLGGSFFFAWLTV
                     ALPLQISPIIATKMDPQLAGLLDYSSNIEFTSRFGVALAWILPFTVIVGIAQIPVTEP
                     AVFSTSFFGKIGPLLFGMLVISIGGVITDSLINAQFRSAIIALDKTAQFVLDNRGNEN
                     VDPLVAREIHARAFGTVEESMTDTRYLFVGSFDETLGDINILVKFEKAWVTCEVLYNQ
                     PITCKPVQSE"
     gene            5043..6917
                     /locus_tag="PLE39_14240"
     CDS             5043..6917
                     /locus_tag="PLE39_14240"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE69540.1"
                     /translation="MEKSPKTFNLRFVLLLGTVIVLLLIQFSALLPNVTTPVERIEYS
                     TRDVLMRLRGAQDPSQNIVIVEIDDFSFNWTGYQWPWPRSYLAGIVDQINKGGGKVVG
                     VDIFLSEPDEDTANDQAFATALANAPAAVGVVQIFESPVDTFSISTLLQAQPVYQSAL
                     DANGITAFTRDEDAIVRSVQAYDFYKDKVYYHWAFEVARLYLDVEPPTQPTDTSIQFN
                     GQSVPLRAGHVLVNFAGPSRTYTTYRAADVHDGITLEENPDAFRGKIVLIGATTVTLQ
                     DMYPTPFSTQIPTAGVEIVASTIDTLITGQYLTEAPPWLALALTILAAVIASLISRSN
                     RPTLTIVLLVLVMAGYAVGGVVLFLRQRYILPFVVPQVMLFLGIVLPTLEQAVSQELE
                     KRRVRNLFSRFISPEMVDQMMSTQDLNSLNKRSNISIIFSDIRGFTTLSEKLAPEDVV
                     ALLNPYLEAMSKVIYKHGGTVDKYEGDAIIAFFGEPVPFKDHAIRALRASLDMRKALD
                     ELKAQWEKEGRPTQIEMGIGINSGDVFVGLLGSAQRINYTVIGDNANLASRLQDLTKT
                     YAWPILISESTYQQVKDEFDAEFADAVTVKGKTKPVNVYKVLGYKGAPPAEQLQSWQK
                     "
     gene            7012..7659
                     /locus_tag="PLE39_14245"
     CDS             7012..7659
                     /locus_tag="PLE39_14245"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNE69541.1"
                     /translation="MANFTNARAFMKAEKIPDIIVGRLPVYLRALQRMADNGLKTTSS
                     QELGEHVGISAAQIRKDISQFGDFGKQGTGYSIAYLLDKLREILKVDRIWEVALVGAG
                     DMGHALARYQGFTNRGFHIVMVFDNDKEKIGQKINGFTIEDTEKMSERLKSAGIKIAM
                     LTVPAPAAQSVTDKLVQAGVKAILNYAPINLNVPNGVKVQYIDPSTHLQRMTYYL"
     gene            7856..8701
                     /locus_tag="PLE39_14250"
     CDS             7856..8701
                     /locus_tag="PLE39_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNE69542.1"
                     /translation="MKIFGKRLFRPRSIWIRKIVQWFFFLLIALISVNHTLVENGGGI
                     PFLSTASLHALCPFGGVVTIYQYATVGTFVQKIHESAFILMIIGFVLAFLFGPVFCGW
                     VCPLGTIQEWVAKLGHKFFKRKYNHFIPAKLDSVLRYARYIILAWVVYVTATSGTLIF
                     AEYDPYFALFNFWSTEVAVGGLIILGATLILSLFIERPWCKYACPYGAVLGITNLFRV
                     FKIKRAESTCKADGACSIMCPMNIAVDTVQTVRDHQCISCLECTSEAICPVAKTVVFT
                     TGAVK"
     gene            8698..9474
                     /locus_tag="PLE39_14255"
     CDS             8698..9474
                     /locus_tag="PLE39_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69543.1"
                     /translation="MNLTSKTLAAIILVILFGGIVFSTAMGWWATESSKVAATYTEGE
                     FAGQANPADIRGSYTFGDVEKNFGIPAAIMAQAFNIQTENPAAFAAKELETIYGESEF
                     EVGTASVRLFVAFYNGLPFDLSTDMYLPQSAADLLKSRNLTAEQSAYLESHSIPNLAA
                     ASDSPEVAPAQTAESAPEAEATQAVVTTEASTERLVKGKTSFQEVLDWGVSQEIIEQI
                     MGMPMPNPLTKIKDYCLEKGLDFETIKTALQAEVDKAGQQ"
     gene            9558..10223
                     /locus_tag="PLE39_14260"
     CDS             9558..10223
                     /locus_tag="PLE39_14260"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE69544.1"
                     /translation="MSKIWNASEYDLSRRRLIPCYDLFYSTAAELAARSVKDAAPVIL
                     DLGAGTGLLSEFVLNKIGPASLYLLDESAEMLAKAQQRLAEYNPLVFIQQMTGPLPSA
                     KFHAVISSLAIHHLTDEDKRDLFKRIHESLLPGGVFINAEQILGPTEWQDQMFEDMHL
                     NGARALGSDENEIRAAQERMKADRCATLEEQVTWLREVGFKNSGTFFQHFRFAVYAGW
                     KGQ"
     gene            10307..10510
                     /locus_tag="PLE39_14265"
     CDS             10307..10510
                     /locus_tag="PLE39_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69545.1"
                     /translation="MRKRNWRLVGFGIFLLILAIGFYFFMATIAPTSLDPVALMKIVG
                     STSGTVGGLSIAIILIGLIGKKA"
     gene            complement(10562..11569)
                     /locus_tag="PLE39_14270"
     CDS             complement(10562..11569)
                     /locus_tag="PLE39_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNE69546.1"
                     /translation="MALFSKDLGVDLGTMFTRIADGAQVLLEEPTIVAIEVDDQKMVA
                     VGMEARDMFGRVPESIEVARPLRNGVIADYEISETLLQYLLQRVSGSMRIFRPRVMIS
                     VPYGVTSVERRAVYEAVMEAGSREAFLIQQPLAAAIGIDLPINSPSGNMLISLGGGCT
                     ETAVMAMYGIVAADTLRAGGMDLDEAIVNYVRRKYGVVIGQATAEQLKIRIGAAVPQD
                     TENSMEVQGQDQVTGLPRPVTLTTGEIVEALQEPLKQIVESGRKVLEKTPPELVSDII
                     DRGVALCGGGALLRGVDKLLTKSLGIPAYLVDNPLTCVVQGAVKSFGMLNVIRRNLPQ
                     V"
     gene            complement(11575..12015)
                     /locus_tag="PLE39_14275"
     CDS             complement(11575..12015)
                     /locus_tag="PLE39_14275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNE69547.1"
                     /translation="MTIRCEIVSQDRTVFTGDVDIVVLPGAGGEMGVLPKHAPVLTTL
                     KYGIVRVRKAGKEELFTVAGGVAEVQPDIVTILADAAENVEEIDEARADAARKRAEEA
                     LKKGLPADTDAYLAMEAALRKSNLRLDAVRRYRKNSRSPYSSEQ"
     gene            complement(12084..13484)
                     /gene="atpD"
                     /locus_tag="PLE39_14280"
     CDS             complement(12084..13484)
                     /gene="atpD"
                     /locus_tag="PLE39_14280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNE69548.1"
                     /translation="MANANGRVVQILGGVVDVEFPNGDVPGLFEAIEVNRENKEPLIL
                     EVQKHLGNSWVRTVAMDSTDGLQRGLPARSTGAPITVPVGPATLGRIFNVLGNAIDKK
                     GDVQAASHYPIHRAAPAFEEQSTRVEVFETGVKVIDLIAPFTKGGKTGIFGGAGTGKT
                     VIIMELIRSVASVHEGNSVFAGVGERTREGTGLYREMIEYGVMKDTVMVYGQMNEPSG
                     VRLRVALTALSMAEYFRDQGKDVLLFIDNIFRFSMSGSEVSALLGRMPSAVGYQPTLA
                     TEMGELQERITSTRTGSITSMQAVYVPADDYSDPAPVATFTHLDATIALERSIVEKGI
                     YPAVDPLASTSRILDPNIVGEEHYRTAREVQRVLQRYKDLQDIIAILGIDELSEDDKL
                     VVSRARKIERFFSQPFFVAERFTGISGRYVTIQDTVRGFREILDGKCDDLPEQAFMMA
                     GTIEDVRERAEKLSKA"
     gene            complement(13497..>14391)
                     /gene="atpG"
                     /locus_tag="PLE39_14285"
     CDS             complement(13497..>14391)
                     /gene="atpG"
                     /locus_tag="PLE39_14285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNE69549.1"
                     /translation="MASAREMRLRIKSVKNISQVTRALETVSASKVRKAINAVMATRS
                     YATKAWQVLRHVAEQPGRDSLHPLLVKRSNVKNALVVVITGDRGLAGAYNSNVIRYTA
                     QKFDQYSVPVKYIAVGRKGRDLLFRRRKDVIADFSNLPAAPTFMDVSAIGRLAVEEYN
                     KGNVDEVFLIYTDFINMGRQVATVKKLLPLELEGEGLVEDFEHKASSGPSAAYEYEPD
                     QREILDEIIPRFTALQVYQAILESQASEHAARMIAMRNATDNAKELVSALQLVYNKVR
                     QQTITNDILDIVGGAEALAAK"
BASE COUNT         3621 a         3889 c         3490 g         3391 t
ORIGIN      
        1 tcggttcatg tcacactctc tcctttggtt tgttttgcct gattgtatcg tatgccttgc
       61 tgaagattaa gccccatagg ggatggaacc atgaccaatt aaaattcctg aaacaaggtg
      121 ctggctgggc tattttcctt ttatgccaag gtaatcgcta tagccgcctt gatacccgcc
      181 cagctgacca tcttcgatga ccagtaacct ttccactgtg cggtcgagaa aataacggtc
      241 atgagaaatg acaagcaccg ttccttcgaa atcttcaagg gcttgttcca aaacttcagc
      301 agaggcaata tcaagattat tggttggttc atcgaggagc aggaaattcg ccccagagag
      361 caccaccaaa gccaattgca ggcggctgcg ttccccgcct gagagttcgc cgatcttttg
      421 cgagacctgt ttgtaggtga ataaatatcg caaaagaaat gccgtcgccc gtgattcgct
      481 catctcaccc gcataacgca cagcttccac aacggtttgg ttgaaatcga gggtttcatg
      541 ttcctgagcg taatgcccaa tggaaatgct gggaccgatc ttgatctcgc ctacatcggg
      601 tgtttctttt ccaaggatca tgcgcaatag aacagacttc cctgcaccgt ttgggccgat
      661 caaacccaca cgttcgccat gccagatggt ctcgttgagg tttgcaaaca cctgcttcga
      721 gccaaaggat tttgaaactc ctgcaagttc aagcaccttg ttcgaaccgc gccagccatt
      781 caaccgcaac tccattcgtt tgcgatcaag tacgggcttc tcgatcttgt ccattttatc
      841 gagccgcttt tgcatggtct tggccttggc ggcaaatttt tcgttgtcgt aaatctttcc
      901 ccaaatggca aagcgcttga tcgcaatttg catgcgggtg atctcatgct gctgcgcgcg
      961 gaaaagctcc tcctgccgtg ccatacgcgt ttccttgtct gcgatgaagg aggaataatc
     1021 gccctcaaag acggtcaact tgccatcttc caattcagcg atatgtgtca cagcggcatc
     1081 gagcagataa cggtcatgtg aaatcagcac taccgtccca tcgtattcgc ggatgagttt
     1141 ttcaaggaac atcttgccgg ggaggtcgag gtgattatca ggctcgtcca acagcaaaat
     1201 atcggggcgc accaaaagca ggcgcgccaa cccgaccaat tttttctgtc cgccgctcaa
     1261 aatagacagg gacttgttta attcactctc agccagaccc agcccgagca acatgctgcg
     1321 tacgcgcgca ggatacgaat caccgccaag ggcatgatat tcttcaataa gtttgtgctg
     1381 caactcaagc gcctgctgaa gcctgcgctc gttgccatag acctcagcat cgcccaaact
     1441 ggcttccacg cgttccagat cagcctgcac ttctgccaca cgcggattcc ctgcaagcgc
     1501 ggcttcaaac gccgtcagcg taagatcaag ctcgggatgt tgagacaggt atcccgttgt
     1561 aatgaggcgg gcgcgggtga tcgatccgcc caattcggga gaatgttccc cttcgataag
     1621 tttgaagaga gatgacttcc ccgcgccatt cgcaccgatg agaccgatct tctggccgtt
     1681 ctggatctcc caattgagat tttcaaagat gcgctttgcg ccaaggacaa gtgtaatgtt
     1741 tgaaagttga agttggatca tgttgacctt taaataaaaa atgccgcagt cggcctgcgg
     1801 catgtgaaag ttgacaaaag gctttcagcg tacaggcgcg attccagaag gctgcgcgga
     1861 aatgaagcca cgtacgccaa tgacaagggg aaatttgcct gcactgaatt tgtaaatcat
     1921 atgcgcgatt ataactgaaa actgtggcaa aatatagcca tgcctgacca actcaacacc
     1981 ctcagccagt cttcgcttca agattattac gattgtccgc gccgatttga attgcgtcat
     2041 ttacagcacc tggtttaccc agccgttgaa accgaacctg cgcttgaaaa tgaaaaacac
     2101 cagcaggaag gcgaatactt tcaccgcctt gtgcagcagc atcttatcgg catcccagcc
     2161 gagcaggttg gcaggctcgc aaacacaccc aacctgcagc gttggtggga aaattattta
     2221 agtgcaaaag acctggcgag cctcagaaat cattcaaagc tatacgctga gacaactcta
     2281 tctgcgccgc tcggaaacct tcgccttgtt gcaaaatatg accttatcgc ggtaagcagc
     2341 gaacaggtat tgatctacga ttggaaaacc taccgcaaac gcccaaagaa tgactggttg
     2401 agcatccgct ggcagacgcg ggtgtaccgt gccctgcttg tgcaggcggg cgcgcatttg
     2461 aacggcggaa aaccgtttgc gcccgaacag atcgaaatga tctactggtt cgcagatttc
     2521 cccaacgaac cagcccgatt cacctacaaa gccgaacaat tcaaacgcga ttgggatgca
     2581 cttgtaaaaa tttcggatga aataaaaacc gcatcagact tcccgaaaac tgatgatgcc
     2641 tcgaaatgca gttactgccc ctacagatcg tactgcaacc gcggcattcg ggcaggtgat
     2701 gccgccgatg cagaactgga aaccgaagcc gaggaattgt tcgatatcaa tttcgagcag
     2761 atcggtgaaa tcgcattttg agctcaggct taaagaaaaa gcgccaggca aagtcgctta
     2821 acagactggc gattttgaaa aaggcaaatt ttttattggc ttttcgcggc cccgcattga
     2881 agggtttctg gctttgcaca gccataggca ttcgccattg tgccgctatg gtaaaattcc
     2941 tctcgcccgc ccccgtagct cagtggacag agtgtcggcc tccggagccg aagagcgcag
     3001 ttcgagtctg cgcgggggcg cctgtttttg catgcagagg gatttctcaa gtgagggcgt
     3061 gcaatttgat cttctggatg cattcaagtg gcccttagaa acattctctt tattatcttt
     3121 gtaatgcttg tttgcacagg ctgcggtgtg aaactcaccg gagaaggtgc atccacagca
     3181 gcgccagatt ttgttacctc caccctgcct ccctcggcac ctcccctggc aacacaaacc
     3241 ccaatcgcac aacaatccat cgcccctaca gcaatggcca cccacctgcc cactgaaggg
     3301 ataacgaata cgcaggtgaa tatccgcgca gctacatcca ccgcaagcac atcgtttggg
     3361 gtgatcggcg catttgaaac ggtgcaggtc cttggcaaag atgccagcgg aagttggtat
     3421 caaatcatgt acgcagggtc gcccaatgga aacggctggg tccgcgccga gtatgtgcag
     3481 gtcaatgatg cggggaagat cctgcctgtt gaaggcctca caggcaacgg cgggcttgtc
     3541 ctcgaagccg tcaacctccg cagcggacca gggtcagatt ttgagtcgtt gggtgttctc
     3601 agttcaaaag atgcggtact cgtcactggc aaagacccga gcggtgcatg ggtgcaggtc
     3661 gaagtgacag gcgcggaaaa tttgaagggc tgggtcacgg tcaaattttt gcaggtggaa
     3721 aaccttgagg caatccctgt gatcggggaa attgccagcc caacatccct ccccgccgaa
     3781 gaagtttcga cggccattcc gccagcctca caggatggag actcgcttca atctccgctg
     3841 acagttgtcc gcttttcttc aacagaatca cgggccatgc agattcaggg caatgtctcc
     3901 gcacccgctg gcgacacgga agattggata cagttttcaa caggcggcaa tggtgtcgta
     3961 attgaggcgc tttgctccag tgatacactc accatagaat tgtggtttga cggacaacgc
     4021 acggatggtt tttctcagat ttgcggggaa aaacagttcg taactgttga gccgaacgga
     4081 aagtacgttt tacgaattct cgaaaccagc acaaatcagt ttcgaaacac agatttcatt
     4141 ttgaccatag aggacattca tcaaccatga aaagcttacc aagcatcgac accatgggcg
     4201 cacgcccgga tattattcgc aataaacgca tctttggaat gatctacgga atcatggcag
     4261 ggttggcctt tgctggctct tcctggggct gggatgggta cacgctctcc caattccacg
     4321 ggtattttcc gtggaatgtg ctggtcacag gggcgcttct gtgcgccatt gtcggcgggt
     4381 tcattggctg gttgacggcc cgcatggaaa gcagcctggc aggcgtgcta ttctggctgg
     4441 gaggatcgtt tttcttcgca tggctcaccg ttgccctgcc gttgcaaatc tcccccatca
     4501 ttgccacgaa aatggatccg caactggcag gcctgctgga ttacagcagt aacatcgaat
     4561 tcacctcccg ttttggagtg gcactcgcgt ggatcctgcc atttaccgtg atcgtgggca
     4621 tcgctcaaat ccctgtcaca gaacctgccg tattttcaac atcgtttttt ggaaagatcg
     4681 gtccgctcct gtttgggatg ctggttatca gcatcggcgg cgtcatcaca gacagtttga
     4741 tcaatgccca gtttcgaagc gccatcattg cactcgacaa aacggcccaa ttcgttttgg
     4801 ataaccgcgg caatgaaaat gtggatccgt tggttgcgcg tgaaatacat gcccgcgcct
     4861 ttggaaccgt ggaagaaagc atgaccgaca caagatattt attcgttgga agtttcgacg
     4921 aaaccctggg agatatcaac attctggtaa agtttgaaaa agcatgggtg acctgcgagg
     4981 tcctctacaa ccagccgatc acatgcaagc cagtccaatc agaataacga ggaatacaga
     5041 gcatggaaaa atcgccaaag actttcaact tgcgctttgt tctgcttttg gggacggtca
     5101 ttgtcttatt gttgatccaa ttttccgcgc ttctgccgaa tgtgaccacc cctgtggagc
     5161 gcatcgaata ttccacgcgc gatgtgttga tgcgcctgcg gggcgcacag gacccctccc
     5221 aaaacatcgt gatcgtggaa attgacgatt tctcctttaa ttggacgggg tatcaatggc
     5281 cctggccccg atcgtatctt gctgggatcg tagaccaaat caacaagggt ggcggcaaag
     5341 tggtgggcgt ggatattttc ctctccgaac ccgatgaaga tactgcaaat gatcaggcct
     5401 ttgcgacggc gctggcaaat gcgcccgccg ctgtgggcgt ggtgcagata ttcgaaagcc
     5461 ctgtcgatac tttttcgatt tccaccttat tgcaggccca acctgtttat cagtccgctc
     5521 tggatgcaaa cggcatcacc gccttcaccc gcgatgaaga tgccatcgtc cgcagcgtgc
     5581 aggcatatga tttctacaag gataaagttt attatcactg ggcctttgag gtcgcaaggc
     5641 tgtatcttga tgtcgaacca ccgacccagc ctacggatac cagtatccag ttcaacggtc
     5701 agagtgtgcc gctgcgtgcg gggcatgtac tggttaattt tgcgggtcca tccagaacct
     5761 acaccaccta tcgcgcggcc gatgtccacg atgggatcac gcttgaagaa aatccagacg
     5821 cgtttcgcgg caagatcgtc ttgatcgggg caacgaccgt caccttgcag gatatgtacc
     5881 ccaccccctt ttccactcag atcccaaccg caggcgtgga aattgttgcc agcaccatag
     5941 atacgctgat cacaggtcaa tacctgaccg aagcgccgcc ctggctggcg ttggcactga
     6001 ccatcctagc tgctgtgatc gcctcgctga tctcccgctc caatcgccca acgttaacaa
     6061 ttgtgctgct tgtgttggta atggcaggtt atgcggtagg cggcgtggtg ttatttttac
     6121 gtcagagata catcctgcca ttcgtcgtac cgcaggtcat gttgtttttg gggattgtcc
     6181 tgcctacttt ggaacaggcg gtttcgcagg agttggaaaa gcgccgtgtc agaaatcttt
     6241 ttagccgttt catctccccc gaaatggttg accagatgat gtccactcag gatctaaact
     6301 cactgaacaa acgctccaac attagcatca tcttttcaga tattcgcgga tttaccactc
     6361 tttcagaaaa gctcgcgcct gaagatgtgg tggccttgct caacccctat ttggaagcca
     6421 tgtccaaggt catttacaaa catggcggca ccgtagacaa atacgaaggc gacgccatca
     6481 ttgctttctt cggcgagcct gtcccgttca aagaccatgc catccgcgcc ctgcgcgcgt
     6541 cactggatat gcgaaaggcg ctggatgaat taaaagcaca atgggaaaaa gaaggccgcc
     6601 ccacccagat cgaaatgggc attggcatca actccggtga cgttttcgta ggcctgcttg
     6661 gctcggcaca gcgtatcaac tacaccgtga tcggcgataa tgccaacctc gcctcccgcc
     6721 tgcaagacct gacaaaaacc tatgcgtggc cgattctcat cagcgaaagc acctaccaac
     6781 aggttaaaga tgagtttgat gcggagttcg cggatgcggt cacggtcaaa gggaagacaa
     6841 aacctgtaaa tgtgtacaaa gtacttggtt acaaaggcgc accacccgct gagcagctac
     6901 aatcctggca aaagtagcaa aaacgaaaaa acggcaccct aaaaaatgcc gttttttcgt
     6961 ttgtacgata cttgtgtgga attgtacaaa acctcttttt atggtagagt tatggcgaat
     7021 ttcacaaacg cgagggcatt catgaaagct gaaaaaatac ctgatattat cgtcggccga
     7081 ctccccgttt atctacgcgc cttacagcgc atggcagata atggcctgaa gacaacttca
     7141 tcgcaggagc tcggggaaca tgtaggcatt tccgccgccc aaatccgtaa ggacatctcg
     7201 caattcggcg atttcggcaa acaaggcacg gggtattcca tcgcctacct cttggataaa
     7261 ttacgtgaaa tccttaaagt agatcgtatt tgggaggtgg cccttgtagg tgcaggtgat
     7321 atgggtcacg cgctggcccg ttatcagggc tttaccaacc gcggctttca catcgtcatg
     7381 gtcttcgaca atgacaagga aaagatcggt caaaagatca atggctttac gatcgaagac
     7441 actgaaaaaa tgagtgaacg cctgaaatcg gctggaatca aaattgcaat gctcaccgtc
     7501 ccagcaccag ctgcacagag cgtcaccgac aagttggttc aggctggcgt caaagcgatc
     7561 ctcaactacg cccccatcaa cttgaatgtt cccaatggtg tcaaagtaca atacattgat
     7621 ccatccacac atttacagag aatgacctat tatttgtaaa aagtagaaaa caggggcgaa
     7681 ccaaaattac aaaacctgcc gaacatatgc gttcggcagg tttttcttta ccttccacac
     7741 aatctctaat tacctgcttc ccaaaaaacg ctttaccgaa tcttcatatt ctctttgaac
     7801 ggcctaaaca caaattcttt acactgtgtc caataaaaag aacaaggaga atatgatgaa
     7861 aatttttggt aaacgcctct tccgtccccg cagtatctgg atcagaaaaa tcgtgcaatg
     7921 gtttttcttt ctacttattg cgcttatctc ggttaaccac acattggtgg aaaacggagg
     7981 cggcattccc ttcctgtcca ctgcatcact gcatgcactc tgtcctttcg gcggcgtggt
     8041 caccatttac caatatgcca cagtgggtac ctttgtacag aaaatccatg aatctgcgtt
     8101 cattctcatg atcattggct tcgtgctggc ctttcttttc ggccccgtat tttgcggttg
     8161 ggtttgcccg cttggaacca ttcaggaatg ggttgccaaa ctgggacata aattcttcaa
     8221 gcgaaaatac aaccacttca tcccagccaa actcgattcc gtcctgcgat atgcacgcta
     8281 catcatcctc gcctgggtgg tgtacgtcac cgccaccagc ggtacactga tctttgccga
     8341 atacgacccc tattttgccc tcttcaattt ttggagcact gaagtagccg tcggcggctt
     8401 gatcatcctt ggcgccacac tcatactttc gcttttcatc gaacgcccgt ggtgcaaata
     8461 cgcctgtcca tacggagcag ttctgggaat taccaacctc ttccgtgttt tcaagatcaa
     8521 acgcgccgaa tccacctgta aagccgatgg cgcctgctcc atcatgtgcc caatgaatat
     8581 cgccgtggat acagtccaaa ccgtacgcga tcaccagtgc atctcatgcc tcgaatgcac
     8641 atccgaagcc atttgccctg tcgcaaaaac tgttgtcttc accacaggag ccgtaaaatg
     8701 aacctcacat ccaaaacact cgccgcgatc atcctcgtca ttttatttgg cggcatcgtc
     8761 ttcagcacag ccatgggctg gtgggccact gaaagcagca aggttgcagc cacgtacacc
     8821 gagggagaat ttgcaggtca ggccaacccc gccgacatcc gcggctcgta caccttcggc
     8881 gatgtggaaa agaacttcgg tatccccgct gccatcatgg cgcaggcatt caacatccaa
     8941 accgaaaacc cagccgcctt cgccgccaag gaactcgaaa ccatctatgg cgagagtgaa
     9001 ttcgaagtag gcactgcatc tgtcagatta ttcgtggctt tttacaatgg cctgccgttc
     9061 gacctcagca ccgacatgta tcttccacaa agcgcggcgg atctgctaaa aagcagaaat
     9121 ctcaccgcag aacaatctgc ttatttggaa tctcacagca tccccaacct cgccgccgca
     9181 tcagactcac ccgaagttgc tcctgcccaa acagcagagt cagcacctga agcagaagca
     9241 actcaagccg tagtcacgac cgaagcatcc actgaaagac tcgtcaaagg caagacatcc
     9301 ttccaggagg ttttggattg gggcgtgagt caggagatca tcgagcagat catgggtatg
     9361 ccaatgccca atccgttgac caagatcaaa gactactgcc tcgaaaaagg gttggatttt
     9421 gaaaccatca aaacagccct gcaagccgaa gtggataaag cagggcagca atagccatca
     9481 gaaaacaggc acaggcggtt caaaacgaac cgcctgttta ttttcaaaaa agtgaatcga
     9541 agatacaatc ggccgctatg agcaaaatat ggaacgcctc tgaatacgac ctttcacgcc
     9601 gccgccttat cccctgctat gacctgttct actccacagc cgcagaactt gctgcccgct
     9661 cggtcaaaga tgcagcgcct gtcattcttg atcttggcgc agggacaggt ctgcttagcg
     9721 aattcgtact caataagatc ggccctgcct ctctatactt actggacgaa tccgctgaga
     9781 tgctggcgaa agctcaacaa aggctcgcgg aatataaccc gttggtgttc attcagcaaa
     9841 tgaccgggcc cctgccgtct gccaaattcc acgccgtcat ttcctcactg gccattcatc
     9901 accttacaga cgaagacaag cgcgatcttt tcaaacgcat ccacgaatct ctgctgcctg
     9961 gcggggtatt catcaacgcc gaacaaatcc tgggacccac cgaatggcag gaccagatgt
    10021 ttgaagatat gcacctcaac ggcgcgcgcg ctctgggcag tgacgagaat gaaatccgcg
    10081 ctgcacagga gcgtatgaaa gctgacaggt gcgccacgct tgaagagcag gttacatggt
    10141 tgagagaagt tggtttcaaa aactcaggga cattctttca gcatttccgt tttgcagttt
    10201 acgcaggctg gaaaggacaa tgattttttc atttcccaaa tcactctgct ttgggtaaaa
    10261 tcaaaatcca gagacaaaat ccattattaa aaggagtctt acaaatatga gaaaaagaaa
    10321 ctggagattg gtcggctttg gaatttttct acttattctt gccatcgggt tttatttttt
    10381 catggcaacc atcgccccca cctcgcttga cccggtcgcc ttgatgaaaa ttgtaggctc
    10441 tacctcggga acagttggag gcctcagcat cgcaatcatc ctcattgggc tgatcggcaa
    10501 gaaagcataa aaagccagcc agtacgcaca aaaaaggctc ccatcaggga gcctttgatt
    10561 tttacacctg cggcaggttt ctacgaatta cgttcaacat tccaaaactc ttgaccgcgc
    10621 cctgcactac acatgtcagc gggttatcga ccaaataagc tggaatgccc aaagacttgg
    10681 tcaacagctt atccacaccg cgcaagagcg cgccaccgcc gcacaaagcc acgccgcgat
    10741 caataatatc ggaaaccagt tcaggcgggg tcttttcgag caccttacgc ccagactcaa
    10801 cgatttgctt caacggttcc tgcaaggctt ccacaatttc gcctgtggtc aaggtgacgg
    10861 gccttggcag acctgtgact tgatcctgcc cctgcacttc catgctattt tcagtatcct
    10921 gcggtacggc cgcaccgata cggatcttca attgctcggc cgttgcctga ccaatcacca
    10981 cgccatattt gcggcgcaca taattgacaa tggcttcgtc caggtccatg cccccagcac
    11041 ggagcgtatc tgcggcaaca attccataca ttgccattac agcagtttca gtacacccac
    11101 caccaaggct gataagcata ttgcctgagg gggaattgat tggcaagtca atgccgatcg
    11161 ccgcagccaa aggctgctga atcaggaacg cctctcggct gcctgcttcc atcacggctt
    11221 catacactgc acgacgttca acgctggtca caccatacgg cacagagatc attacacgcg
    11281 ggcggaagat acgcattgac ccgctcacac gctgcaaaag atactgaagt aatgtttcgc
    11341 taatttcata atcagcgatc acgccattcc ggagcgggcg ggcaacttcg atactttccg
    11401 ggacccttcc aaacatgtca cgggcttcca tgccaactgc gaccattttc tgatcatcca
    11461 cctcaatggc gacgatggtc ggctcctcca acagcacttg cgcgccgtcg gcgatacggg
    11521 tgaacatggt acccaggtca acaccaaggt ctttggaaaa gagtgccact atgtttattg
    11581 ttccgatgaa tagggcgaac gtgagttttt gcggtaacga cgcactgcat caaggcgtaa
    11641 attggatttt cgcaatgcag cctccatggc gaggtaggcg tcagtatcgg caggcaagcc
    11701 cttcttaaga gcttcttctg cacgcttgcg tgcggcgtcg gcccgggctt catcaatttc
    11761 ttcgacattt tcagcagcat cagccagaat ggtgacaata tctggctgca cttcagccac
    11821 gccgccagct acagtaaaga gttcttcttt acccgccttg cggaccctga caatgccata
    11881 cttcaaagtg gtgaggacag gcgcatgctt gggaagcaca cccatttcgc cccctgcacc
    11941 aggcaggaca acgatatcca catcacccgt gaacacggtg cgatcttgag aaacgatttc
    12001 acaacgaatg gtcatataaa aaccttttgg agattaggaa attggagatc agccctggct
    12061 gatctccgat cgctaattac caattacgct ttagacagct tctcagcgcg ttcgcgcaca
    12121 tcttcgatgg taccagccat catgaatgcc tgttcaggca ggtcgtcgca cttgccatca
    12181 aggatctcgc ggaagccgcg gacggtatcc tgaatggtta catagcggcc agagatgccg
    12241 gtaaagcgtt cagccacgaa gaaaggctgc gagaagaaac gttcaatctt acgagcgcgg
    12301 gacacaacca gtttgtcgtc ttcggagagt tcatcaatac cgagaatggc gataatgtcc
    12361 tgcaagtctt tgtagcgctg gagaacacgc tgcacttcac gagctgttcg gtagtgttcc
    12421 tcacccacga tgttaggatc aagaatacgg gaggttgaag cgagcggatc cacggcgggg
    12481 taaatacctt tttccacgat ggagcgttcg agggcgatgg tcgcatccaa gtgggtaaag
    12541 gtcgccacag gagcgggatc ggagtagtca tccgcgggca cgtacacagc ctgcatggag
    12601 gtgatggagc cggtgcgggt ggaggtaatg cgttcctgca gttcacccat ttcagtagcg
    12661 agagtcggtt gataacccac cgcagaaggc atacgtccaa gaagcgcgga cacttcggaa
    12721 ccagacatgg agaagcggaa aatgttatca atgaagagca acacatcctt gccctgatcg
    12781 cggaaatatt ccgccatcga gagcgcggtc aacgccacgc ggaggcgtac accggaaggc
    12841 tcattcatct gtccgtagac catcacggtg tctttcataa cgccgtattc gatcatttca
    12901 cggtacagac cagttccttc acgggtgcgc tcgcccacgc cagcgaacac agagttacct
    12961 tcatgaacgg aggccaccga gcggatgagc tccatgatga taacggtctt gcctgttcct
    13021 gcaccaccga aaatgcccgt cttaccaccc ttggtgaagg gggcgatcag gtcaatgact
    13081 ttgacgccag tttcgaagac ttccacacgt gtggattgtt cttcaaacgc aggcgccgca
    13141 cgatgaatgg ggtaatgaga tgccgcttga acatcaccct tcttgtcgat cgcattgccg
    13201 agaacgttaa agatacgacc aagggtcgcg ggtccaaccg ggaccgtaat cggggcacct
    13261 gttgatctgg cagggagtcc acgctggaga ccatctgtcg aatccatggc cactgtacga
    13321 acccagctat tgccaagatg tttttgaact tcaagaataa ggggttcctt gttttcacga
    13381 ttcacttcaa tcgcttcaaa gagcccgggg acgtcaccat tggggaattc gacatccacc
    13441 acaccaccta aaatttgtac aacacggccg tttgcatttg ccatgatttc ctccagttat
    13501 ttcgcggcca gcgcttctgc gccgccgaca atgtccaaaa tatcgtttgt gatcgtctgt
    13561 tgacgaactt tgttataaac caactgcaac gcgctgacga gttcctttgc gttgtcggta
    13621 gcattgcgca ttgcgatcat gcgggcagca tgctcacttg cctgtgattc aaggatcgcc
    13681 tgatacacct gcaaagcagt aaaacgcgga atgatctcgt ccaaaatttc acgctggtcg
    13741 ggttcatatt catacgccgc agaaggaccg ctggatgcct tgtgttcgaa atcttccacc
    13801 aaaccttcac cttccaattc aagcggaaga agtttcttca ctgtagccac ctgacgaccc
    13861 atattgatga agtcggtata aatgagaaac acctcatcaa cattgccttt gttgtactct
    13921 tccacagcca ggcggccaat ggcagataca tccataaaag tgggggctgc aggcaaattg
    13981 ctgaagtctg caatgacatc tttacggcgg cggaagagta agtctctacc cttgcgtcca
    14041 accgcaatat atttaacggg aacagaatat tggtcgaatt tttgagccgt ataacgaatc
    14101 acattggaat tgtaagcacc tgcaagtccg cgatcaccag tgatgaccac aaccaacgca
    14161 ttcttgacgt tggagcgctt gacgagcaac ggatgcaaag aatcgcgtcc tggttgttcg
    14221 gcgacatgtc gcaacacctg ccatgctttc gtcgcgtagg aacgcgtcgc catgaccgca
    14281 ttgattgcct tgcggacctt gctggcgctc actgtttcca gcgcacgtgt aacctgtgaa
    14341 atgttcttta cgctcttaat tcggagccgc atttcacgag cggaagccat a
//
LOCUS       DAOQJC010000103        14380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2029_length_14380_cov_8.646353, whole genome shotgun sequence.
ACCESSION   DAOQJC010000103 DAOQJC010000000
VERSION     DAOQJC010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14380
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2029_length_14380_cov_8.646353"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLE39_14290"
     CDS             complement(<1..368)
                     /locus_tag="PLE39_14290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69550.1"
                     /translation="MSIHQFHLRRAGMEDAPAVTECVHSAFRHYIERIGREPGPMLMD
                     YAHEIREHQVWVVEQNGQIIGALVMCIKEEGFLLDVIAVEPFHQGMGVGKLMLEFAES
                     EAQRQGFDSIYLYTHEKMTE"
     gene            480..2219
                     /locus_tag="PLE39_14295"
     CDS             480..2219
                     /locus_tag="PLE39_14295"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE69551.1"
                     /translation="MKLIREYWDLLSTYLKPQRLQVLGLGVILCAGIGLQLFTPRVLR
                     DFIDLALSGGEYDLLVRKATLFFTMVLVTQVTATAATYMSERVAWTATNLLRVDLAAH
                     CLRLDMSFHQKHSSGSLIERVEGDVDALSNFFSQFIVRVIANAVLIIGVLVMLFREDW
                     RAGLGLSIFAGLAIYAMLRVRQIGVPRWLALRQKSAEAFGFLGETLHGTEDIRANGAV
                     RYILARFDEILRAWFPLRRRAMVAFSMIWTTTLLLFTFGTGFSFGLGYVLWSAGAITA
                     GTVYLIFNYTEMMRQPIEQVRAQMEDLQRASASIERVRELFALKSSIKDGDHSLESQG
                     ALSVKFNNVMFSYGEEISALSGIDFHLEAGKVLGLLGRTGSGKSTMARLLLRLYDPNS
                     GQILLGGVPTRAAKLADLTRRVGLVPQEVQVFGATVRENFTLFDPAIPDEKILAAIHE
                     FELSPWFDSLPRGLDTELGPGGGLSAGQAQLLNFARVALRDPGLVILDEATSRLDLAT
                     EALIDRATARLLKGRTGIIIAHRLHTVQKADMILILESGAIQEIGSRHELLANPASRF
                     SALLKTGLEEVLA"
     gene            2216..3967
                     /locus_tag="PLE39_14300"
     CDS             2216..3967
                     /locus_tag="PLE39_14300"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE69552.1"
                     /translation="MKTHRILWEMIRYRPWLYTVNAVLWTLIHLAPLIPGLIIQRFFN
                     VFGAETTPASTVWALILLLIVTAVGRGGLFIGGIFVDQRHRFAMSALLRRNILAAILE
                     LPGASALKHTPGDALSRLREDANYAEDAISWTLDAIGMGSFAVVSIGILLSISTRITL
                     FVFVPLVGVIALSQMAATRIHHLRKTSREATGRVTSAISEMFDAVQAIKVAGAEDHVI
                     THYGRLGNIRRSAMLKDRLMTQLLDSVSASAVNLGTGVILLLSVSSLDAHLPLGDFAL
                     FVYYLGFVTEFTQFFGRFLAQYRQAGVSFERMIELVDGLPPERIVSHRPLDLTSTAPL
                     YLDPEPASASLQRLAARDISYRHPNSGRGLDRVNLSLVRGSFTVVTGRVASGKTTLLR
                     VLLGLLPREGGRIDWNGEPVNDPGTFFVPPRCAYIAQVPWLFSGTLRENILLGLKENP
                     TALTEAIRLSVFEQDLAQMPDGLETVIGPKGVRLSGGQVQRAAAARMFVRQPDLLVMD
                     DLSSALDVETEEKLWQRLAAMRDTTCLVVSHRRAAYRQADHIIVLKDGRVEAEGTLND
                     LLKSSPEMQSLWEQQAS"
     gene            complement(3983..8881)
                     /locus_tag="PLE39_14305"
     CDS             complement(3983..8881)
                     /locus_tag="PLE39_14305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE69553.1"
                     /translation="MHPKPLYDSSLTLVRDENGHSHDLLLALSRAAQSIQRARTAEDF
                     YRAVGNEVKSLGGETTLLLINQHSPTLSIAYTSYSPSLIRKAEKITGLSLHEYQIPFS
                     AETVYGRAIHNRKAVFVESVKEAMLEIVPSPISRWIDSLISMFKLGHGILAPLLVDDE
                     TLGLLKINGTFLNQSDIPAMEVFAAQIATGLSNIRLVQKLQDELYARQKAEESLHRSR
                     KTLLALGQAAQSIQKTHTPEDIYRVVGEQIKALGLEATILTLNSNRQTLCHSYTTLST
                     DIIQNAEKMAKVSAQGYCWQIDPNGTYNRIIFSGKSEFIHKSEDLFAEALPKFMWPMT
                     KALMKILKIGQGIIAPLRVDEEDLGLLVVFGNEAISEEDCPAIDSFAGQVAISLRNAH
                     LAKKVEAEFLEKKQAEEAVRRAKEHFEALIEKAPDGITLLGMDGKVKYASPSAKRLFD
                     YSEEDITNLTPMEFIHPEDLDLVLETMNKLVQNPAFTPTIQYRYKRKDGVYYWVESTF
                     SNLLSDPNVEAVVINFHDITQRKLVEKALFESEKYYRALIENASDGILVVNEEGKISY
                     ESPSVTRMLGYEPYSLLGTNAFELIHPDDMAAIIEAFTQGSNTPNFVHRGEYRLIRKN
                     GEWGYFEIVSHYLLTDPAIKGIVINGYDITERKQAEIALQESEARWQFALEGPGDGVW
                     DWNVESNQVFFSRRLKTMLGYDEAENWNTLHEWDNRLHPDDRENTYQSINLHFENKTP
                     YYQSEHRLLCKDGTYKWILDRGKVTEWTDEQKPRRMIGTHTDITERKQVEQALKTSEE
                     RYRALYENIPSMYFTADSEGRIISINKFGVEKLGYSEAELRDHPLLEVFYPDDHTMIQ
                     EQFTTCLQNVGQMVQMETRKIRKDGSLLWVRESARAVYDAHEQLVVLLTCDDITESKK
                     IEAALRTSEEKSNALYRMVRLMTDNLPDLVWAKDMDGRYLFANKAIAEKLLIATDTNE
                     PLGQTDLYFAQRQRTAHPEDPAWHTFGELCINSDAVIHASQQAQRFEEFGNVKGEFLF
                     LDVYKAPFLDENGNMIGTVGIGRDVTYEKKLAEEHRLAQQALAASEAELRALFSSMQD
                     TVLVIKRDGTYSRIAPTRPGKFYIPPERVVGKHLTDFFPQPWVKVFQEVIEKVLTTKL
                     TQHIEYSININGETPWFEASISPMGEDATLWVARDISERKQVETKLQLQSAVLEAVAN
                     TVVITDRNGLIQWTNSSFKNLTGFEPIEAIGKNPGELVKSGIHDKDFYRKLWETILSG
                     RIWQDELVNRRKDGSLYFEEMTITPLKDAQNEISHFIAVKQDITKRKKAENDLMQSEQ
                     AYRTLFENMPIGLYRTLASGEILDANPALVRMFGYPDRATLLQQKPEDLYADPNANQK
                     FNEAIRQNGTLSGYESEFRRYDNTNFWAEDYVHVINDVWGNPLYYEGSLIDITERKRA
                     EDELRRANKNLETTHRELQQMLAHEQVLARTDGLTGLYNRRYFFELAVREFNMSARYQ
                     HKLTIILFDVDGFKQANDTFGHDLGDRILAKIAQVAAAQIRDVDILARYGGDEFIILL
                     PQTDTKQAFLVAERIRENVSTISMETGNNVPLSVTLSMGVAELQTSPQDKSIEEVIRH
                     ADQALYKAKKNGRNHAYIYLDD"
     gene            complement(9107..9553)
                     /locus_tag="PLE39_14310"
     CDS             complement(9107..9553)
                     /locus_tag="PLE39_14310"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HNE69554.1"
                     /translation="MNFSPITDDLFIGTTPSSEDYNHLRGLGVQLVINMRVEYRPRKD
                     PHPTPLELLWLPTFDSPLVPIPMRALHRGANAALETIQNGGKVYAHCAGGVHRGVTMG
                     ACILIAQGMEPMPAMDLIKTKRPLADPFAFYIKPRILKFANQWKSS"
     gene            9653..10525
                     /locus_tag="PLE39_14315"
     CDS             9653..10525
                     /locus_tag="PLE39_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69555.1"
                     /translation="MKKNLSLFLLLVLTACSSAAPVPPTVTPTATATAIPTHTPLPTA
                     TLTSTPVPSGACESPLLPLSTGNQWTYRVTTDDGESTYALKTLERDDGRNTVIKVEFT
                     DQKNNVSVVEPVVCIDSAIENFPLFVVSMYFSGYLEKDFNTYHDSGTYAPNYDILDFN
                     HWSMHWDMYYLTEDTAQLINPTDGQSLFVLQSSFIEMSFTLDGTREAVTVPAGDFPNA
                     LKAGYTFSFPVTIVLQDAMTGGTLTVKTTQWYEPYVGLVRAKVDSTSLRFYGQSVPMQ
                     LNSLIELVEFERGE"
     gene            complement(10532..11797)
                     /locus_tag="PLE39_14320"
     CDS             complement(10532..11797)
                     /locus_tag="PLE39_14320"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE69556.1"
                     /translation="MDTVIKNITLIDGISHAPIADAVIVIGEGKIIYAGPASGWEDAG
                     VESKVWDLRGRFVLPGLIDAHVHLSGSGEVDSQFAVPTGPMALKILSNAQKNLAAGIT
                     TVRDVGGWSELEFDVRRAIQRGEFAGPRMCLAGRFISITEAGADYYAGMYRVADGVDE
                     VRKAVREQVKNGADVIKMGVTGAVLVESGEPGATHFNFDEMQALVQEAKKFGKRVAMH
                     AHGIDGIRKAVEAGVDTLEHGTYLYQDPNVIQHMAERGIFLLPTLKVGWDIILAEKPN
                     IPDWIMDKNKASQDDALKSLKMAYEAGVPIAMGSDVGTPLNYHGENALEIHWMHEAGL
                     STMDAIIAGTGNAARALGWDSWLGTLEAGKVADLIIHEKNPLDDLRVLTDKESLRFVL
                     KDGLIVASHLEDENSAPKELFAQNLLRVG"
     gene            complement(11918..13042)
                     /locus_tag="PLE39_14325"
     CDS             complement(11918..13042)
                     /locus_tag="PLE39_14325"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNE69557.1"
                     /translation="MADKADIERYFANRQKEIDGAALYRTLAETEKQPQMAEVYQRLA
                     LSEEKHAATWEKKLEELKVPIPPRSISWRAKSMIWLTKRFGPQFVLPTITSNEQIDSK
                     AYDGQIEAEAQEFSLEEKSHARMLSIAAHSKAGLPGGTVAQLEGRHKAGGGNALRAAV
                     LGANDGLVSILSLTMGVAGATSSNSDVLIAGLAGVLAGAGSMALGEWLSVQSSRELYE
                     HQIKIETEEIAANPEEEQEELALIYQSKGLPEERARELASHMMADKSSILDTLAREEL
                     GIDPEELGGSAYEAAFTSFILFAVGAFFPVFPYFFWSGATAIYISLVVSAVGLFLIGA
                     AITLMTGRSVWFSGTRQVVVGIAAAALTFGIGKLIGVSLG"
     gene            13321..>14380
                     /locus_tag="PLE39_14330"
     CDS             13321..>14380
                     /locus_tag="PLE39_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69558.1"
                     /translation="MQDQPAAVQGSVHVEGNVVDSNIIIGSNNTIIIGGRQVTFRADW
                     FYGHRYGDIENFTGRTAELKTLDDWLGNDKESLLMIRALGGFGKSVLAWQWFNNRVDK
                     AKWQTAVWWSFYEKESGFESFLAETLNHLGVEVKTSARQQLNDLLEAMRGTNILIVLD
                     GFERLLRQYGQMDAALQSDDEDADAKHDPSQRDCSSPLTETFLRGLSGAGLKSKVLMT
                     TRLCPRVLESADGKLLKGCREEPLAAFLPEDAVTYFHNEGIKATRAEVIEACAAYGYH
                     PLSLSLLVGLINADHERRGDIAAVKNLEIFADVKARRHHVLERAYGSLAPERRDLLSK
                     ISCFRGSMEYAVLKKVFPS"
BASE COUNT         2969 a         3676 c         3987 g         3748 t
ORIGIN      
        1 ttctcggtca tcttttcgtg ggtgtaaaga tagatgctgt caaatccctg ccgttgagct
       61 tcagattcag cgaattcgag catgagcttg ccgacgccca ttccctgatg gaatggttcc
      121 acggcaatga catccagcag aaaaccttct tccttgatgc acatcaccag tgcgccgatg
      181 atctgtccgt tttgctccac gacccatact tgatgttcgc ggatttcatg ggcatagtcc
      241 atcagcatgg ggccaggttc ccgaccgatg cgttcgatgt agtgtcgaaa ggcgctgtgt
      301 acacattccg tgacggcagg tgcatcttcc atgccagcgc ggcgcagatg aaattgatgt
      361 atgctcacag gcattgcctc cgggtttcat tgtacggttt tcaacaagca tggtcaagca
      421 cggtgattct tgaaatcgct tatgattgaa cgaccgattt agcaagagga gcataccctg
      481 tgaaattgat ccgcgagtat tgggatttgt tatccaccta cctgaagccg cagcgattgc
      541 aggtgctggg gctgggtgtg attttgtgtg cggggatcgg cttgcaattg ttcaccccgc
      601 gtgtgctgcg tgattttatc gatctggcgc tttcaggtgg agagtacgat ctgcttgtgc
      661 gcaaggcaac tttgttcttt acgatggtgc ttgtcacaca agtgaccgcc actgccgcca
      721 catacatgag cgagcgcgtt gcatggacgg cgaccaatct cctgcgggtg gatttggcgg
      781 cacactgcct gcggttggat atgtcttttc accagaagca ttcgtcgggt agtttgatcg
      841 agcgcgtcga gggcgatgtc gatgcgcttt ccaatttctt ttcccaattc attgtgcggg
      901 tcattgccaa tgccgtgctc atcatcggcg tgctggtgat gcttttccgc gaagattggc
      961 gcgccgggtt ggggctttcc attttcgcgg ggcttgccat ttatgccatg ctgcgcgtcc
     1021 gccagatcgg tgtgccgcgc tggctggcat tgcgccagaa atcagccgag gcctttggct
     1081 ttctcggtga gaccctgcat ggcacggaag atattcgtgc caatggcgcc gtcagataca
     1141 ttttggctcg tttcgatgag atcttgcgtg catggtttcc cctgcgccgc agggccatgg
     1201 tcgctttcag catgatctgg accaccacgc ttttgctttt cacctttggc actggttttt
     1261 cttttgggct tgggtatgtg ctgtggtcgg cgggtgccat caccgcaggg acggtgtacc
     1321 tcattttcaa ttacaccgag atgatgcgcc aacccattga acaggtccgc gcccaaatgg
     1381 aagacttgca gcgtgccagc gccagcattg aacgggtgcg cgaattgttc gcgttgaaat
     1441 cgagcatcaa ggatggagac cattcgcttg aatcacaggg tgcgctgtcg gtgaaattta
     1501 acaacgtcat gttcagctat ggcgaggaga tatctgcgct aagcggaatc gattttcatt
     1561 tggaggcggg caaggtgttg ggattgttgg gccgcaccgg cagtggaaaa agcaccatgg
     1621 cgcggttgct gctccgtttg tacgacccga actccgggca gatccttttg ggcggggtgc
     1681 cgacccgtgc tgccaaactc gcagatctga cacgtcgtgt ggggttggtg ccgcaggaag
     1741 ttcaagtgtt cggcgcgacc gtgcgggaga atttcacgct cttcgaccca gccattccag
     1801 atgaaaagat attggccgcc attcatgaat ttgagctttc accctggttc gattccctgc
     1861 cgcgtggatt ggatacggaa ctcggcccgg gcggtggact ctcggccggg caggcccagc
     1921 ttttgaattt tgcgcgcgtc gccctgcgtg acccggggct ggtcattctc gatgaagcca
     1981 cttcacgcct cgaccttgcc accgaagcct tgatcgaccg tgccaccgca cgccttctga
     2041 aaggacgcac aggcatcatc attgcacacc gcctgcatac cgtccaaaaa gcggacatga
     2101 tcctgatcct cgaaagcggc gcaattcagg aaattggatc gcgccatgaa ttgcttgcca
     2161 accctgcttc gcgtttttcg gctttgctca aaacaggttt ggaggaggtg ctggcatgaa
     2221 aacccatcgc atcctttggg agatgatccg ctaccgcccg tggctgtaca cggtcaatgc
     2281 agtcttgtgg accttgatcc accttgcgcc gttgatcccc ggcctgatca ttcaacgttt
     2341 cttcaatgtg tttggcgctg aaacgactcc agcctccacc gtgtgggctc tcatcctttt
     2401 gttgatcgtc accgccgttg ggcgcggcgg acttttcatc ggcggtattt ttgtagacca
     2461 acgtcaccgc tttgccatga gcgcgttgct gcgccgcaat attctggcag ccattcttga
     2521 actgccgggc gcctcggccc tgaaacacac ccccggtgat gcgcttagcc gcctgcgtga
     2581 agatgccaac tatgccgaag acgccatcag ttggaccctg gatgccattg gcatgggttc
     2641 gtttgcggtc gtttccatcg gcattttgtt gagcatcagc acgcgcatca cattgttcgt
     2701 gttcgtccct cttgttgggg tcattgctct ttcgcaaatg gcggccacac gcatccatca
     2761 cttgcgtaaa acaagccgcg aggccacagg gcgggtgacc agtgccatca gcgaaatgtt
     2821 cgatgccgtg caggccatca aagtggcggg cgcagaagac catgtcatca cacattacgg
     2881 ccgcctcgga aacatccgcc gttctgccat gctcaaagac cgcctgatga cccaactgct
     2941 ggattcggtc agcgcaagcg cggttaacct cggcacgggt gtcattcttc tgctctctgt
     3001 ttcctcgctg gatgcgcacc tgcccctcgg cgattttgcg ctctttgtgt attacctcgg
     3061 ctttgtcacc gagttcacgc aattcttcgg gcgtttcctc gcgcaatatc gtcaggcagg
     3121 agtttctttt gagcggatga tcgaactggt ggacggcctg ccccccgagc ggatcgtgtc
     3181 ccaccggcct ttggatttga cttcaactgc gcctctttat ctggaccctg aacctgcttc
     3241 agcgtccttg cagagactcg cagcaagaga catttcatat cgccacccca attccgggcg
     3301 cggtttggat cgggtcaatc tatcgctggt acgcggctcg tttacagtgg taaccggcag
     3361 agtggcatca ggcaagacca ccctgttgcg tgttctattg gggttgcttc ctcgtgaggg
     3421 aggccgcatt gattggaatg gggaaccggt aaatgacccc ggtacgttct ttgtgccgcc
     3481 gcgctgcgcc tacattgctc aagtgccgtg gttgttcagc ggaaccttgc gcgaaaatat
     3541 tttgctcggc ttgaaggaaa atcccactgc attgaccgaa gcgatccgcc tttctgtttt
     3601 cgaacaggac cttgcgcaaa tgcccgatgg gttggaaacg gtcatcggcc ccaaaggggt
     3661 gcgcctctcc ggcggacagg tgcagcgtgc cgccgctgcg cgcatgttcg tgcgccagcc
     3721 agacctgctg gtcatggacg acctttccag cgcgctcgat gtggagaccg aggaaaaact
     3781 ctggcagcgg cttgcagcga tgcgtgatac cacctgtttg gtggtgtcac atcgccgggc
     3841 ggcataccgg caggcagatc acattattgt tttgaaggat ggcagagtgg aggcggaagg
     3901 gacgctcaat gacctgctaa agtcctcgcc tgaaatgcaa agtttgtggg agcagcaggc
     3961 ctcttgaagt tgttagaccc gatcaatcgt ccagataaat ataggcatgg ttgcggccat
     4021 tcttcttggc cttgtacaaa gcctgatcgg cgtgacggat gacctcttca atggatttat
     4081 cctgcggcga cgtttgcaat tccgcaacgc ccatgctgag ggtgacggac agggggacat
     4141 tattccctgt ttccatggaa atggttgaga cattttcacg gatgcgctcg gcaacaagaa
     4201 aggcctgttt ggtgtcggtt tgtggcagga ggatgatgaa ttcgtcgccg ccgtagcgtg
     4261 caagaatatc cacgtcacgg atttgggcgg cagcgacttg cgcgattttg gccaggatcc
     4321 ggtctcccag atcatggccg aaagtgtcgt tggcttgctt gaatccatca acatcgaaca
     4381 ggatgatggt cagtttgtgc tggtaacgtg ctgacatgtt gaattcacga acggcaagtt
     4441 caaagaaata acggcggttg tacaggccgg tcaggccgtc tgtgcgggcc aggacctgtt
     4501 cgtgggccag catttgctgt aattcacggt gggtggtttc gagatttttg tttgcacggc
     4561 gcaattcatc ttctgcgcgt ttgcgttcgg tgatgtcgat caggctgcct tcataataaa
     4621 gtgggtttcc ccaaacatca ttgatgacgt gaacataatc ttcagcccag aagtttgtat
     4681 tgtcgtagcg gcgaaattcg gattcgtatc ctgaaagcgt gccgttctgc ctgatcgctt
     4741 cattgaattt ttggttggcg ttagggtcgg catacaggtc ttccggtttc tgttggagca
     4801 gggtggcgcg gtctgggtag ccgaacatgc gcaccaatgc agggttggca tccagtattt
     4861 ctccgctcgc gagggtacga tacaggccaa tgggcatatt ctcgaacaat gtccgatagg
     4921 cctgctcgga ttgcatcagg tcattctctg ccttttttcg tttggtgata tcctgtttga
     4981 cagcaatgaa gtggctgatt tcgttttgtg cgtctttcag cggggtgatg gtcatttctt
     5041 caaaatacag gctgccgtct ttgcggcggt ttaccaactc atcctgccag attctgccgg
     5101 aaaggatcgt ctcccaaagt ttacggtaga aatccttgtc atgtattcct gatttgacca
     5161 attctcccgg gttttttccg atggcctcaa taggttcgaa gccggtcaag tttttgaaag
     5221 aggaattggt ccattggatg aggccgttgc ggtcagtgat gacaacggtg ttggcaactg
     5281 cttcgagaac agcgctttgc agttgaagtt ttgtttcgac ctgttttctt tcgctgatgt
     5341 cgcgggccac ccacagggtg gcatcttcac ccatcgggga gatggaggcc tcgaaccagg
     5401 gagtttctcc attgatatta atcgagtatt cgatgtgttg ggtcagtttt gtggtgagta
     5461 ccttttcgat tacttcctgg aagaccttaa cccatggttg ggggaagaaa tcggtgaggt
     5521 gtttgccgac cactctttcg ggggggatgt agaattttcc cgggcgcgtg ggggcgatcc
     5581 tgctgtaggt gccatcacgt ttgatgacga ggaccgtatc ctgcatggag gagaacaggg
     5641 cgcgcaattc cgcttctgag gcggcaagcg cctgttgagc caggcgatgt tcttcggcca
     5701 gttttttctc gtaggtcaca tcgcggccaa tacccacggt gccgatcatg ttcccgtttt
     5761 cgtcaaggaa gggggctttg tacacatcga gaaacaggaa ttcgcctttg acatttccaa
     5821 actcttcgaa acgttgggcc tgctggctgg catggatgac ggcatctgag ttgatgcaca
     5881 gttcaccgaa ggtatgccag gcggggtctt cggggtgggc agttctttgt cgctgggcaa
     5941 aatacaggtc ggtttgaccg aggggttcat ttgtgtcggt tgcgatcagt aatttttcag
     6001 cgatcgcctt gttggcgaag agataacgtc cgtccatatc cttggcccaa accagatctg
     6061 ggaggttgtc ggtcatgagg cggaccattc gatacagggc gttcgatttt tcttcgcttg
     6121 tgcgcagggc cgcttcgatc tttttgcttt ctgtgatgtc gtcacaggtg agcaggacaa
     6181 ccagttgttc atgtgcatca tatacggcac gggctgattc tcgaacccat aaaagtgagc
     6241 cgtctttccg tatctttctt gtttccatct gaaccatttg acccacattc tgcaggcagg
     6301 ttgtaaattg ttcctgaatc atggtgtggt cgtcggggta aaaaacctca agcagaggat
     6361 gatctcttag ctcggcttcc gagtatccga gtttttcaac cccaaatttg ttgatggaaa
     6421 tgatcctgcc ttcagaatcg gccgtgaagt acatggaagg gatgttttca taaagcgcgc
     6481 gataacgttc ttcactggtt ttgagtgcct gttcgacctg ttttcgctcg gtgatgtcgg
     6541 tgtgcgtgcc gatcatgcgt cgaggttttt gttcgtctgt ccattcggtg actttgccgc
     6601 ggtcgaggat ccatttgtag gtgccgtctt tacataacaa gcggtgttcg ctttgataat
     6661 agggcgtttt gttttcgaag tgcaggttga tggattggta ggtgttctca cggtcatcgg
     6721 ggtgcaggcg gttgtcccat tcgtgcaggg tattccagtt ttcggcctca tcatacccaa
     6781 gcatggtttt cagacgacgt gagaaaaaga cctggttgga ttccacgttc caatcccaaa
     6841 ccccgtcgcc ggggccttcc agtgcaaatt gccagcgtgc ttcgctctct tgcagggcta
     6901 tttcggcttg cttgcgctcg gtaatgtcat aaccgttgat cacgattccc ttgatggcgg
     6961 ggtcggtaag caggtagtga cttacaattt caaagtaacc ccattctcca ttttttcgaa
     7021 taaggcgata ttcgccgcga tggacgaagt tgggggtatt cgatccttgt gtgaatgctt
     7081 ctataatggc ggccatgtca tcggggtgga tgagttcgaa ggcatttgtc ccgagcaggc
     7141 tgtacggctc ataacctaac atgcgtgtga cagagggact ttcgtaactg atcttccctt
     7201 cctcattcac aaccaggatg ccgtcggagg cgttttcgat caatgcgcgg taatactttt
     7261 cgctttcgaa cagggctttt tcaacgagct tgcgttgtgt gatatcgtgg aagttgatca
     7321 ccactgcttc cacgtttggg tcgctcagca ggttgctgaa ggtgctctcc acccaataat
     7381 agacaccgtc tttgcgtttg taccgatatt gaatggtggg tgtgaaggca gggttttgaa
     7441 ccagtttgtt cattgtttca agaacgaggt cgaggtcttc aggatgaatg aactccatgg
     7501 gggtgaggtt cgttatatcc tcctcgctgt aatcgaacag tctttttgca gaaggactgg
     7561 cgtatttgac cttcccatcc atgcccagca gggtgatgcc atcgggggcc ttttcgatca
     7621 aggcttcgaa atgttccttg gcccgtcgca cagcttcttc ggcttgtttt ttctccagaa
     7681 attcagcttc cacttttttg gccaggtggg cgttccgcaa actgatggct acctggcccg
     7741 caaaggaatc gatggcgggg caatcctctt cactgatggc ttcatttcca aatacgacca
     7801 acagaccaag gtcttcttcg tccacccgca ggggtgcgat gattccctgc ccaatcttca
     7861 ggattttcat gagagccttg gtcatgggcc acatgaattt tggaagggct tctgcaaaga
     7921 ggtcttccga cttgtgaata aattctgact tgcctgaaaa aatgatgcgg ttataggtgc
     7981 cgtttgggtc aatttgccag caatatcctt gtgcagatac ctttgccatc ttttcggcat
     8041 tttgaatgat atctgtggaa agggtggtgt agctatggca gagtgtctga cgattgctgt
     8101 tgagtgtgag aatggtggct tcaagcccca gcgccttgat ctgttcgcca accacacgat
     8161 agatgtcttc cggggtgtgg gttttctgga tcgattgcgc cgcctgcccc aatgcaagga
     8221 gcgttttacg gctgcggtgc agggattctt ccgctttttg gcgggcgtat agttcatcct
     8281 gcaatttttg caccagacgg atattggata accctgtcgc gatctgggct gcaaaaactt
     8341 ccatggcggg tatatcagat tggtttagga acgtgccgtt gatttttaac aggcccaggg
     8401 tttcatcgtc caccaatagg ggcgcgagga tgccatgccc cagtttaaac atggatatca
     8461 atgaatctat ccagcggctg atgggggagg ggacgatctc caacatggcc tctttgactg
     8521 attcaacgaa tactgctttt ctgttgtgaa tggcgcggcc atagacagtt tctgctgaaa
     8581 aaggaatttg atattcgtgc agagacagcc ctgtgatctt ttcggccttg cggatcaggg
     8641 atggagaata ggaagtatag gcaatggaaa gtgtggggct gtgctgattg atcaaaagta
     8701 aggttgtttc tccccccaga gacttaactt catttccaac ggcacgatag aaatcttcag
     8761 ccgtgcgcgc ccgttggatg gattgcgctg cacggcttaa agccaataaa agatcgtggc
     8821 tatgaccatt ttcatcccgc accaatgtga gggaggaatc atagagaggc tttggatgca
     8881 tcgaaacctt ccttgcagat tcgctttatg tgacaaggtg tgggcagctg aacagatgga
     8941 tctggttcag gcgaatcagg tttaagatat atccgccgtg tataagaaaa aatgatgaaa
     9001 aattactaaa aagggaatca aacttttgat gggcggcgtt tattggaata taaaggagtt
     9061 aaccttcatc gtcaagtatt tatgtgtgtt gatgatgact tgcgggtcag gatgatttcc
     9121 attgatttgc gaatttcagg attcgcggtt tgatgtagaa cgcgaacggg tcagccaatg
     9181 ggcgttttgt cttgatcaga tccattgctg gcattggttc cattccctgg gcgatgagaa
     9241 tgcaggcacc cattgtcacc ccgcggtgga ctccgcctgc gcaatgggca tagaccttgc
     9301 cgccgttttg gatcgtttcg agtgcggcat tggctccgcg atgcagggca cgcatgggga
     9361 tgggcacaag gggagaatca aatgttggaa gccagagcag ttcgagggga gtcggatgcg
     9421 ggtctttcct cgggcggtat tccacgcgca tgttgatcac caattggact ccgagtccgc
     9481 gcagatggtt gtagtcttct gatgaagggg ttgtgccgat gaacaggtca tcggtgatgg
     9541 gggagaagtt catgcgggaa atggtatcac aaggactggc gcctgagata tgttctttgt
     9601 cacatgcaat gctggagcgc gcggcctata atgattgtaa ggagaacttg ccatgaaaaa
     9661 gaatctgagc ctgtttcttt tactggttct tactgcctgt tcctccgctg cacctgtgcc
     9721 cccgaccgtg acccccaccg cgactgccac tgcgattcca acccataccc ctttgccgac
     9781 cgccacattg acaagtacgc ccgtcccaag cggtgcatgt gaaagcccgc ttctgccatt
     9841 gagcacaggc aatcagtgga cttaccgcgt tacaacggat gatggagaat cgacctacgc
     9901 tctgaaaacg ctggaacggg acgacggacg caacaccgtg ataaaagttg aattcaccga
     9961 ccagaaaaat aacgtgtctg tggttgagcc tgtggtttgc attgactcgg ccattgagaa
    10021 ctttccgctg ttcgtggtga gcatgtattt ttcaggatat ttggaaaagg atttcaatac
    10081 ttatcacgat tcaggtacct atgcccccaa ttacgacatc ctcgatttca accactggtc
    10141 aatgcattgg gatatgtatt atcttaccga agatacggcc caactcatca acccaacgga
    10201 tggccaatcc ctgttcgtgt tgcagtcttc gttcattgaa atgtccttca cgttggatgg
    10261 gactcgtgaa gcggtgaccg tgcccgctgg ggattttcca aatgcgttga aggcggggta
    10321 tacattttca tttcccgtga cgatcgttct gcaggatgca atgactggcg gcacgctgac
    10381 cgtaaaaacg acccaatggt acgagccgta tgtgggtctt gtccgcgcga aggtggattc
    10441 gacctccctt cggttttatg gacaaagtgt gccgatgcag ttgaacagcc tgatcgagtt
    10501 ggtggagttt gaaagaggag agtaggaaaa cttaacccac cctcaacaaa ttctgcgcga
    10561 acaattcctt gggcgcggaa ttttcatctt ccaggtgcga agcgacgatc aacccatcct
    10621 tcagcacgaa gcgcagactc tctttatctg taaggacgcg cagatcatcc agcgggttct
    10681 tttcgtggat gatcaggtct gcaactttgc cagcttcgag ggtgccaagc cacgagtccc
    10741 agccgagggc gcgggcggcg ttccctgtcc cagcgatgat cgcatccatt gtggagagtc
    10801 ccgcctcatg catccaatgg atttcgagcg cgttctcacc gtgatagttc aacggcgtgc
    10861 cgacatctga gcccattgcg atgggcacgc ctgcttcgta ggccatcttc aaacttttta
    10921 gcgcgtcatc ctgcgatgcc ttgttcttgt ccatgatcca atcggggatg ttcggctttt
    10981 cggcgaggat gatatcccag cccactttca aggtcggcag gaggaagatg ccgcgctcag
    11041 ccatgtgttg aatcacgttc gggtcctggt acaggtatgt gccatgttcc aacgtatcca
    11101 caccagcttc cacggccttt ctgattccat ctattccatg tgcgtgcatg gcaacacgtt
    11161 tgccgaactt ctttgcctcc tgcaccagcg cctgcatttc atcgaagttg aaatgggtcg
    11221 caccgggttc gccgctttcc accaacaccg cgcccgtcac acccatcttg atcacatccg
    11281 cgccgttctt cacctgttcg cgcaccgcct tgcggacttc atccacacca tctgccacgc
    11341 ggtacatgcc cgcgtaataa tctgcgcctg cttcggtgat cgaaatgaat cgacccgcca
    11401 gacacatgcg tggccctgca aattctccgc gttggatggc gcgccgcaca tcgaattcca
    11461 gttcgctcca cccgcccaca tcgcgtacgg ttgtgatccc tgccgcgaga ttcttctgcg
    11521 cgttcgaaag gatcttcaac gccatcggcc ccgtcggcac ggcaaattga ctatccactt
    11581 cgccagagcc tgaaaggtgg acgtgcgcat cgatcaaccc cggcaggacg aatcgtccgc
    11641 gcaaatccca tactttggat tcaacccccg catcttccca cccggaagct ggcccagcgt
    11701 agatgatctt cccctccccg atgacaatga ccgcatccgc gatcggcgcg tggcttattc
    11761 cgtcgatgag ggtgatgttc ttgataacag tatccatttg aagtcctgta aagatgcgtg
    11821 gattttgagt catcatattc tatccccgaa ataaaaaatt ctcccccatt tgtgttcttc
    11881 aaatggggga gatgtcccgc ttacgggaca gagggcgtta tcccaaagag acaccgatca
    11941 acttgccgat gccgaaggtc agcgctgctg cggcgatgcc gacgaccacc tgccttgtgc
    12001 cagagaacca gacgctgcgt cccgtcatca aggtgatggc cgcgccgatg aggaagaggc
    12061 cgaccgcact cacgacaagg ctgatgtaga tcgcagttgc accgctccaa aagaaatacg
    12121 ggaagacggg gaagaacgca cccaccgcga acaggatgaa tgaagtgaac gcagcttcat
    12181 aggcagaccc gcccagttcc tcggggtcaa tgcccaattc ttcgcgggcg agcgtatcca
    12241 gaatgctgct tttatccgcc atcatgtgtg aggcgagttc ccgtgcgcgt tcttcgggca
    12301 ggcctttgga ctgatagatc agcgccagtt cctcctgttc ttcttcgggg ttggcagcga
    12361 tctcttcggt ttcgatcttg atctgatgtt catacagttc gcgcgaactc tgcaccgaca
    12421 gccattcccc caatgccatg gaccccgcgc ctgcaagcac acccgcgagc cctgcgatca
    12481 acacatcgct gttcgaactg gtggctccag ccacacccat cgtgaggctg agaatggaga
    12541 ccaatccgtc attcgcgccg agcacggcag cgcggagcgc attgcccccg cccgccttgt
    12601 gacgtccctc cagttgtgca acggtgccgc ctggcaaacc cgctttcgaa tgtgccgcaa
    12661 tggacagcat ccgcgcatgg gatttttctt cgagcgagaa ttcctgcgcc tcggcttcga
    12721 tctgaccgtc gtaggctttg ctgtcgatct gttcgttgct ggtgatggtc ggcaaaacga
    12781 attgcgggcc gaagcgtttg gtgagccaga tcatgctttt tgcacgccat gatatgctgc
    12841 gcggtggaat cggaaccttg agttcctcca gtttcttttc ccatgttgcg gcatgtttct
    12901 cttcgctgag cgcaaggcgc tgatacacct ccgccatctg cggttgtttc tctgtttcgg
    12961 ccagtgtgcg gtacaacgcc gccccgtcga tctctttttg cctgttcgca aaataacgtt
    13021 cgatatctgc tttatctgcc atggttcact ctcgattcgg gtttatgttt ggaaagcgtt
    13081 tttgattttg accacagtgt tgcgaattgc agatttcagc ctgcaatgtc actgtaaaag
    13141 cggactgaag tccgcgctac gacctggttt tggatgtatt ccgttcctct aaaattatgg
    13201 cacaaaatct gtgggtttct tcctgtcata tgtcattgat tatgtgttaa agttggctta
    13261 tcgttgtcac aaacagggaa ggttgatatg gcaaaagata aaaaatccaa gagttcaaag
    13321 atgcaagatc agcccgcagc agtgcaggga agtgttcatg tggaaggaaa tgttgttgat
    13381 agcaacatta tcatcggcag caacaacacc atcattatcg gcggcaggca ggtcacattt
    13441 cgcgcggact ggttttatgg gcaccgttat ggcgacatcg aaaactttac tggcagaact
    13501 gcagaactca aaacccttga tgattggctg ggtaatgaca aagaaagttt gttgatgatt
    13561 cgtgcacttg ggggatttgg aaaatccgtt ttagcctggc aatggttcaa taaccgtgtg
    13621 gataaagcca aatggcagac ggcggtgtgg tggtcgttct atgaaaagga atcgggcttt
    13681 gagagttttc tcgcggagac gctgaaccat ctcggcgtgg aggtcaaaac gtcggcgcgg
    13741 cagcaactca atgatctgct ggaggcgatg cgtgggacaa atattctgat cgtgctggac
    13801 ggcttcgagc ggctgctgcg tcaatacggt cagatggatg cggcacttca atccgatgat
    13861 gaagatgcgg atgcgaagca tgacccatcc cagcgcgatt gctcgtcccc gctgacggaa
    13921 actttcctgc gcggtttgag cggcgcgggg ttgaagtcca aagtcttgat gaccacgcgc
    13981 ctgtgtccgc gtgtgttgga aagcgccgac ggcaaactgc tcaaaggctg ccgcgaggag
    14041 ccgctcgccg ccttcttgcc cgaggatgcg gtgacgtact ttcacaacga gggaatcaaa
    14101 gccacgcgcg cggaggtcat cgaagcctgc gccgcgtacg gctatcaccc gttgagtttg
    14161 agcctgctgg tcggtttgat caacgccgac cacgaacggc gtggcgacat tgcagcggtg
    14221 aagaatctgg aaatcttcgc ggatgtgaaa gccagaagac accacgtgtt ggaacgcgcc
    14281 tacggaagtt tagcccccga gcgcagggac ttactgagca agatttcctg ctttcgcggc
    14341 tcgatggaat acgccgtgct caaaaaggtg ttccccagcc
//
LOCUS       DAOQJC010000104        14317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2042_length_14317_cov_8.005118, whole genome shotgun sequence.
ACCESSION   DAOQJC010000104 DAOQJC010000000
VERSION     DAOQJC010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14317
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2042_length_14317_cov_8.005118"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PLE39_14335"
     CDS             <1..464
                     /locus_tag="PLE39_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69559.1"
                     /translation="GGSVEERTYKNNETSMVVLKGTYDVIVVKGAKQKVVDTVDCTGN
                     TCVVENIVATLTVKFPGLSSVHTYVKVNDGVVGTAAGGSVDERTYKTNEVSIVVLKST
                     YDVVVIKGSKQVIVDAVDCTGDTCVIENILAALTAKFPASGLFAYNNKERR"
     gene            complement(509..>3410)
                     /locus_tag="PLE39_14340"
     CDS             complement(509..>3410)
                     /locus_tag="PLE39_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNE69560.1"
                     /translation="SSFLMRFTSPLYLLLLLLVPITVWLGYPAAGQSRKRDLTSLILR
                     LVIVLCLILAISGLEIVQRGDNLAVVFLVDVSDSMPPQAVDSEVGYIREALQSIGADD
                     QAAVILFGADALVERPMSALDELAEITSVPITNQTDLAEAIQLGLAMFSSGYAKRMVI
                     LSDGAETTGNAAKAAEFAAASDVQIVVVPFVNQVDAEALVTEVDAPTHLRSGEKFDLN
                     VSIQANQSMQATVRVFSQTEMIYEGGHDLHKGLQTLSLPLTAQDPGFVTYQVQVTPQQ
                     DAFYQNNRLDTFSQVEGPPRILVVSPLEGEAIPGGEIRPDETSLLLTSLTSAGFDVRT
                     VTPNLLPADLPTLAQYNSVVLVNVPARQLGRNQMETLQSYVRDLGGGLVAIGGPTSYG
                     VGGYYGTPLEEALPVDMQIKDEKRRPSLTMVFIIDHSGSMSDTSGGVTKLELAKEAAA
                     RSVELLFPNDHVGVIAFDDTASWVVPITDLQNPAEVVNAIGTIQIGGGTDIYAGLLAM
                     SETLPNDPSQTKHVVLLTDGGADITGIPELVKKLYEENGITLSTVGVGNDAAPFLKDI
                     AALGGGRYHFTNNASTIASIFTEETTLATRAYIVEESFFPTLVNSSPILANITEVPPL
                     HGYVASSAKDLAQVILESEKGDPILAAWQYGLGKSVAFTSDATGRWARDWARSEIFPT
                     FWVQAVRYTINDSLNSALQMNIEMQGENASITLDAHDRTGGFLNGYQVEASIVSPNGR
                     EAAQTVTFTQTAPGRYESTFTPTEQGIYLIHFSGTTDSGNGSASFAETTGWALSYSPE
                     YQRIESNPDLLLRLSALANGQVASPLPADVFKHDLQAARATQPMAPFLLLLAALLLPV
                     DIAVRRLIVTKTDLIRLREWVTARFTKTSRAAEPINEKASPQMEALFSAKSRVRENKP
                     AQNEVRPSAQAMPVETESGSGPEKEAQETPPKAEQSASTTSALLARKKNIRK"
     assembly_gap    3411..3510
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3511..>4570)
                     /locus_tag="PLE39_14345"
     CDS             complement(<3511..>4570)
                     /locus_tag="PLE39_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69561.1"
                     /translation="ARQLKVASDSKVGISMENLPATTAVYKAQISDPANANAKLDSLS
                     QDDTAFAIYQSSAARRVLLVSKGNLFLEQLLASLPGIQPFRALPADDGTLQIPNDPFD
                     LYVFDGYIPAELPKSNLLLINPPTNPFFEVGDAFKEMGEFQVNEHALTQFVDWKTVHV
                     LQAHKVSLPNWMNVLITTDDNPLVFAGETNGQRIAALSFDLRESDLPLQIAYPILFSN
                     LINYLAPPSAFDSSQALQAGESLSIVPQTDAKQVIVAAPSGTLYSLPPGQNLFSNTDE
                     LGFYAVNFVTETSTRSEYFAVNLFDEAESNIQPRESLQLGSTSVTPTVSQQIGLQELW
                     KWLAGFALIVLMIEWQVFH"
     assembly_gap    4571..4670
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4671..5209)
                     /locus_tag="PLE39_14350"
     CDS             complement(<4671..5209)
                     /locus_tag="PLE39_14350"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HNE69562.1"
                     /translation="MQFLAPLAFALSALAIPILLLYMLKLRRRQTQVSSTLLWEQLLR
                     DRQANAPWQKLKRNLLLFLQLLILAALVFALARPALQTKVVASGSVIVLLDASASMNA
                     TDVTPSRFEEARKSVLTLINGLTNASSMTLILVAKTPQTLIAAESDSSLLKAALTKAQ
                     VTQGSADWEAAFALAAGATR"
     gene            complement(5251..6132)
                     /locus_tag="PLE39_14355"
     CDS             complement(5251..6132)
                     /locus_tag="PLE39_14355"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNE69563.1"
                     /translation="MLFDESTLRKLNQLTLVASRVRSGAIKGERRSSRRGSSVEFADY
                     RNYTAGDDLRRLDWNIYARLEKPFIKLLEEEEDLAVYILIDGSRSMDWGEQEENKFKY
                     ALRLAAGTGAIALSSGDLLSVGFLQGGRVASEFGPSRGQYALPRMFKFLESLKAGETT
                     NLNTSLRDYSIQPRRAGLVILISDLLTADGYESGLRQLLGRGHEAAMIHILAPDEVDP
                     PLAGDLQLVDIETNFQQDVSIDNGLRNLYRARVQAWRQSIQTDCRKHNVRYLDLVTSL
                     SWDQALLLEMRRSGIAK"
     gene            complement(6170..6541)
                     /locus_tag="PLE39_14360"
     CDS             complement(6170..6541)
                     /locus_tag="PLE39_14360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNE69564.1"
                     /translation="MESKTPQDLRMRTSDFALRIIKLYSSLPKTTEAQVLGKQVLRSG
                     TSIGANYREGHRAKSDSDIVNKFESVLQELDETDYWLDLLVRSGILSAQKAEALIKET
                     NELIAIFTTIVTKIKKRMGKI"
     gene            complement(6576..7559)
                     /locus_tag="PLE39_14365"
     CDS             complement(6576..7559)
                     /locus_tag="PLE39_14365"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNE69565.1"
                     /translation="MSDFSVDTFREYANRIETEVGRVIVGQGDVVRHVLVSILAGGHV
                     LLEGVPGLGKTMLIRTLGQALDLKFSRIQFTPDLMPADILGTEILSDDNGKREFRFRE
                     GPLFSNLVLADEINRATPKTQSALLEAMQEASVTVAQRTYKLEEPFFVMATQNPLEME
                     GTYPLPEAQLDRFFFKVNVDLPNVGEMVEILDRTTGKESPAASKAVTGADIIQMRALA
                     RTTPIASHVKEYIARLMLATHPEDSAASPLVKKYVRYGVSPRGGQALVLGAKVTALLA
                     GRYNVAFEDVHAVAKAALRHRLLLNFDGMAEGVKADDVIEELVNARMKDEG"
     gene            complement(7570..9153)
                     /locus_tag="PLE39_14370"
     CDS             complement(7570..9153)
                     /locus_tag="PLE39_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69566.1"
                     /translation="MSTINHLNHTLQNWIQWLRVQRALTWYLRGLAAALGLSLLFGGI
                     GLAQAKLLKQEFLALVVASVTLLPILSAIIAFFWKVNTTKAARHFDRAFHLEERISTA
                     LELSTADHANEMVQKQLDDAVSAAHRIQPRRDLPLRLKRFDSALALILALLIGVLWFR
                     GDALFAAASRQRAVEQAIAAQQEQIEEIIKEINENEALTAEQKQELTEPLNQALQALD
                     QNPSLEGAVSILTNTGEQLQALNSEQAGQTAQALKEAGSSLQAQEGTPLSSVGQNLAS
                     GNFTQAANELNNMDLSQMSPEELQKLADQLEATAQSLASTNPQLAQAMQQAADAIRNG
                     DLAAAQQALANASQQMANAGQQVTAAQTASQAAGQMQQGAGQLLAAGGGNQSAQANGQ
                     GQGQQGNNSGGQNSSAGSGSGSGDSPDSPQQGNEAGSSPIEQNNGAGDGGESTYEQIY
                     APSLLGGENGENVGLPTSGNDGDVVGTSPTTATDGQSLVPYTNVFTQYEQFYYQAIEN
                     GQVPMQFFDLIRNYFISLK"
     gene            complement(9168..10028)
                     /locus_tag="PLE39_14375"
     CDS             complement(9168..10028)
                     /locus_tag="PLE39_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69567.1"
                     /translation="MNNPIIAKELKGRMRGRQGFVMLTAYLGLISLVIYAVYVYSVRN
                     TYSRSPGFLQEAGKQVFLIVVLLEIILTGFIGPALTSGAISSERERQTYDQLRMSLLS
                     AKDIVIGKLGSSVMYLLLLIVAAIPLQSLAFFLGGVGIEEVLISIWILTLSTINFCTL
                     GLYFSGISKRTSIATILSYTTLILPILLFAAGIYAINENSYLLSNASFEFQRWLLNVL
                     WTIISTNPASAAFVSEAMLIEEQNLFLIQSPVPPMVLVSPWILYSIFSITMTILMIRL
                     IVFYLKRHEP"
     gene            10160..10873
                     /locus_tag="PLE39_14380"
     CDS             10160..10873
                     /locus_tag="PLE39_14380"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE69568.1"
                     /translation="MPLALVTGGAHRLGKAFALSLARMGYSIALHYRASANEADHTVK
                     EIRALGVDCLPIRADLTLPEKIDFLFSLVDEFKTPLKVLVNSAAVMPVGKPQELEIHD
                     WDLALDLNLRAPFMLAQEAAKRMTDGGLIVNITDIGAQKAWSRYPSYTVSKAGLESLT
                     KLLARALAPTIRVNAIAPGLVLPSDVVTEEQWDNLVQKLPLKRAATLDEVTSALEFLI
                     KNEYITGQTIVVDGGYSLL"
     gene            10940..12616
                     /locus_tag="PLE39_14385"
     CDS             10940..12616
                     /locus_tag="PLE39_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69569.1"
                     /translation="MSFKKLPLDLLASIFLIAFTLFYAARFVDFSIPPFEDAAMLMRY
                     AQHLAEGHGIVWNIGEAPVDGATDFLFMAASAALIKLGFTVGQSVRGIGFVSHLLTVL
                     IVYWTNRRVNHTDIPFSLLSGLYLAVGTGFSYVAAYFGTPFFALAAASTWTLGLLLIQ
                     KENPPFSLILAFALSGLVTGLIRPEGVILASLMLLAIIVMRGWKNSVAIIVTFGSVFL
                     LLGGAYFVWRWNYFGYPLPNPFYKKGDSGLHWDSFYNSLSNTLRMCLPFVFVFILGFR
                     SRETSTRTLAYLIPILGFASAFILISDEMNFGARFQYALVPLVLMSWTPLLFSGNQSG
                     LGVLSSLYREVPVGGDQNQLSVRNGRVYLFAGIALFVSLVYYSSYQNCFLTSFQQTCA
                     RPYERDGRYEMGRLLGEYRGKGYVIATTEAGLVPYYSGWTAIDTWGLNDQFIAHNGKI
                     TVEYLDKYKPHIIMFHDYYSPLVPPKLTDANLAQRWFGMTILLKTYAEENGYILAAVF
                     GDSPYDTNYFYVRPDFEDSKKLVGQISGFKDYYWFTTGKRSINYAEFDKP"
     gene            12644..13114
                     /locus_tag="PLE39_14390"
     CDS             12644..13114
                     /locus_tag="PLE39_14390"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE69570.1"
                     /translation="MSETTELAEKLKTEGEKYIEFFSGMNESLWQQEVYTEGEMWTVR
                     NVLSHFVTSERGLVKLFEQIRQGGTGAADDFSIDRYNASQQAKTKDLSPSELLEQYKE
                     VRANSVKWVSGLKDEELEIKGRHPFLGETVLREMVKMLYIHNQLHFRDLKKALK"
     gene            13230..>14317
                     /locus_tag="PLE39_14395"
     CDS             13230..>14317
                     /locus_tag="PLE39_14395"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE69571.1"
                     /translation="MILPPFKLERYFAKYEFTTEYLLCSSDCEAMSIAELLALEPGSA
                     EKFQNVWLGYTESLGSPSLRKEITNLYTTMQPDDILVHTGAGEAIYLFMHAAFEKGDH
                     VIVHSPGYQSLTEVARAVGCDVSPWRAREQDGWALDLDELRHLMRTNTKGIIINTPHN
                     PTGYLMSHADFEAVNKFAQEKGLILFSDEVYRESEYDSADRLPAACDMGEHAVSLGVT
                     SKTYGLAGLRIGWVATKNKKILQAMASLKDYTTICNSAPSEFLAEVAMRNRQILIDRS
                     LGIIKHNLTVVDDLFNRHAALFTWARPKAGSMGFPKLLKGDIEDFCDDLVRKAGVLLL
                     PGSVYDDSRNHFRLGLGRKNLPQAVERL"
BASE COUNT         3172 a         3526 c         3896 g         3523 t
ORIGIN      
        1 caggcggcag tgttgaagaa cgcacctata agaataacga aacctcaatg gtggtactga
       61 agggcactta cgatgttatc gtggtcaagg gcgcaaaaca aaaggtcgtt gatacggtgg
      121 attgcactgg gaatacctgt gtggtcgaaa acattgttgc cacgctgacg gtgaaattcc
      181 ccggactcag cagtgtccat acctatgtca aggtaaatga tggcgtggtc ggcacagctg
      241 caggcggcag tgttgatgaa cgcacctaca aaaccaatga agtttcgatc gtggtgttga
      301 agagcactta cgatgtggtt gtgatcaagg ggtcaaaaca ggtcattgtg gatgcagtag
      361 actgcacagg cgacacctgt gtaattgaaa acatccttgc tgcattgact gcgaaatttc
      421 ctgccagtgg tctctttgcc tataacaaca aagaacgacg ctagctcgat tgcttgaaaa
      481 atgtgccccg aattttcggg gcacattttt attttcgaat attctttttt cttgcgagta
      541 atgcagacgt ggtcgatgcg gattgttctg ccttcggcgg agtctcctgc gcttcttttt
      601 ctggacctga acctgattca gtttcaacag gcattgcctg cgcggatggt ctgacttcat
      661 tttgtgcggg tttgttttcc cgcacacggc tcttcgcact gaacaaggct tccatttgcg
      721 ggctggcttt ttcgttgatc ggctccgcgg cccgactggt tttcgtaaat cgtgccgtga
      781 cccattcacg caggcgaatc agatcggtct ttgtgacgat gagtctgcgg acggcgatat
      841 ccactggaag aagcaaggct gcgagcagca gcaaaaatgg ggccatcggt tgtgtggcgc
      901 gggccgcttg caggtcatgc ttgaacacgt ctgcggggag gggagaagca acctgcccgt
      961 tggccaatgc cgatagacgc aggagcaggt cagggtttga ttcgatgcgc tggtattccg
     1021 gtgagtagga taatgcccac ccggtggtct ctgcaaaaga ggcggatccg tttccagagt
     1081 cagtcgtccc cgaaaaatgg atgaggtaaa ttccctgctc ggtgggtgtg aacgtggatt
     1141 cgtaccgccc gggcgcggtc tgcgtgaatg tgacggtttg cgccgcttcg cggccattcg
     1201 gtgaaacgat gctggcttcc acttgatagc cgttgaggaa cccgcctgtg cggtcatgcg
     1261 cgtcgagggt gatgctggca ttttcaccct gcatttcgat gttcatttgc agcgccgaat
     1321 tgagcgagtc gttgatggtg tagcgcacag cctgcaccca gaaggttggg aaaatttcag
     1381 agcgtgccca atcgcgggcc cagcggcctg ttgcatcaga agtgaatgcg accgatttcc
     1441 cgaggccgta ttgccatgcg gcgaggatcg ggtcgccttt ttctgattcg aggatgactt
     1501 gtgcgaggtc ttttgcgctg gaggcgacat aaccatgcaa aggaggtact tcggtgatgt
     1561 tggcgagaat cggcgacgaa ttgaccaaag ttgggaaaaa cgattcttca acgatgtagg
     1621 cacgtgtggc cagtgtggtc tcttctgtaa agatcgaagc gatggtgctc gcgttgttgg
     1681 tgaagtgata acggccgccg ccaagcgcgg cgatatcttt caggaagggt gcggcatcgt
     1741 taccgacgcc cacagtggaa agcgtgatgc cgttttcttc gtacaatttt ttcacgagtt
     1801 caggaatgcc cgtgatgtcg gcgccgccgt cggtaagcag gacgacatgt tttgtttgtg
     1861 aggggtcgtt gggcagcgtt tcagacatgg ccagcaaacc tgcataaata tcggtgccgc
     1921 cgccgatctg gatcgtgcca atggcattga caacctcagc ggggttttgc aggtcggtga
     1981 tcggcaccac ccaactggcg gtgtcgtcaa atgcgatcac gccgacatgg tcattgggga
     2041 aaagcaattc aaccgaacgc gccgcggctt ccttggcaag ttcgagtttt gtcacaccac
     2101 cgctggtgtc gctcatactt cctgaatggt caatgataaa gaccatcgtg agagaggggc
     2161 ggcgcttttc atctttgatc tgcatgtcta caggcagggc ttcttcgagc ggcgtgccgt
     2221 aatacccgcc gacgccgtaa ctggttggcc cgcctatcgc tacaagcccg ccgcctagat
     2281 cgcgcacata ggattgcagg gtttccattt ggttgcggcc aagctgcctt gcagggacat
     2341 tcaccagcac gaccgagttg tattgagcca gtgtgggcag gtcagcgggt agaagatttg
     2401 gcgtgacggt tctcacgtcg aaacctgccg atgtgagtga agtgagtaac aatgaagttt
     2461 catcggggcg aatttcgccg cccggaatgg cctctccttc gaggggtgat accaccaaaa
     2521 tgcgcggcgg accttcgacc tgcgagaacg tatccagccg attgttttga tagaaggcat
     2581 cctgctgcgg cgtgacctgc acctgataag tgacaaagcc agggtcttgt gctgtgagcg
     2641 gcaggctcaa ggtttgcaga cccttgtgca ggtcatgccc accttcgtag atcatttcgg
     2701 tttgcgaaaa cacgcgcact gtggcctgca tggactgatt ggcctggatc gaaacattca
     2761 gatcaaattt ctcaccgctg cgcaaatggg tcggcgcatc cacttcggtg acgagggcct
     2821 cggcgtcaac ctgatttacg aacggcacga cgacgatctg cacatctgaa gcggccgcga
     2881 attcggcggc cttggccgca ttgccagtgg tttccgcgcc atcagagagg atcaccatgc
     2941 gttttgcata gcccgacgag aacatcgcca gcccaagctg aatcgcctct gccagatcgg
     3001 tttggttggt gatcggcacg gaagtgattt ccgcgagttc atccagcgcc gacattgggc
     3061 gttccaccaa tgcgtctgcg ccgaagagaa tcaccgcagc ttgatcgtcc gcgccgatgg
     3121 attgcagcgc ctcacggatg tagcccactt ccgaatccac ggcttgcggc ggcatggaat
     3181 cggatacatc cacaaggaag acgactgcaa ggttatctcc acgctggacg atttcaagac
     3241 cagagatggc gaggatcaaa cagaggacga tcacaagccg cagtatcagc gacgtgagat
     3301 cgcgcttccg tgattgacct gcggcaggat acccaagcca gaccgtgatc ggcacgagaa
     3361 gaaggagcag gaggtagagc ggagaggtga aacgcatgag aaaggatgaa nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gatggaaaac ctgccattcg atcatcagta
     3541 cgatcaaggc aaatcctgcc agccatttcc ataattcttg caggccgatc tgttgagaga
     3601 cggtgggtgt gacggatgtg ctgccaagct gcaggctttc gcgcggctga atattcgact
     3661 cggcttcgtc gaacaggttg acggcgaaat attcggagcg ggtggaggtt tcggtgacga
     3721 aattcacggc gtaaaagccg agttcatcgg tgttgctgaa caggttttgc ccaggcggca
     3781 gggagtaaag tgtgcccgat ggcgcggcaa cgatgacctg ttttgcatcg gtttgaggga
     3841 caatggaaag gctctcgcct gcttgaagcg cttgcgatga gtcaaaggcg ctgggcggtg
     3901 caagataatt gatgaggttg gaaaaaagaa tcgggtaggc gatctgcaag ggcaggtcac
     3961 tttcgcgcag atcgaatgac agggctgcga ttctctgacc gtttgtttcg cccgcaaaca
     4021 ccagcggatt atcgtcggtt gtgatcagca cgttcatcca atttggcaga gacactttgt
     4081 gagcctgcaa cacgtggaca gttttccaat ctacgaactg ggtgagcgca tgttcgttga
     4141 cttgaaattc gcccatttcc ttgaaggcat ctccaacctc aaaaaaagga ttcgtcggcg
     4201 ggttgatgag cagcaaattc gattttggca actccgctgg gatataacca tcgaagacgt
     4261 atagatcaaa cgggtcgttg gggatttgca gagtcccatc gtctgcgggc agggcgcgga
     4321 agggttgaat gccagggagt gaagccaata attgctcaag gaatagattc ccttttgaaa
     4381 ccaacagcac gcgtcttgcg gcggaggatt gataaattgc aaaagcggta tcgtcttgtg
     4441 agagggaatc cagttttgca tttgcgttgg cagggtcgga aatctgcgcc ttgtatactg
     4501 ctgttgtcgc tgggagattt tccatcgaaa taccgacttt ggaatcggaa gccactttta
     4561 attgacgggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgggttgcgc
     4681 ctgcggccag cgcgaatgcg gcttcccagt ctgcgcttcc ttgcgtgacc tgtgctttgg
     4741 tcagcgctgc tttgagcagg gacgaatctg attcagcggc gatcaaggtc tgcggggttt
     4801 ttgcaaccag gatcagggtc atggaagaag cgtttgtcag tccgttgatc agagtcagca
     4861 cggatttccg cgcttcttca aaacgggatg gagttacatc cgtcgcgttc atcgaagcag
     4921 acgcatctaa caacacgatg actgatcctg atgcgacgac ttttgtttgc agggcggggc
     4981 gtgccagtgc aaagaccagt gcggcgagga tgagtagttg aagaaaaagt aaaagattgc
     5041 gtttgagttt ttgccacgga gcgttcgcct gcctgtcgcg cagaagttgt tcccaaagca
     5101 gagtcgaaga gacttgcgtc tgcctgcggc gcaatttgag catgtacagc agaaggatgg
     5161 gaatggcgag cgcactgagc gcgaaggcga gcggggcgag gaattgcatg gggaaaggat
     5221 gaattatgaa ggatgaaaga agaaagatgt ctacttggct atccctgaac gacgcatttc
     5281 gagaaggagc gcttgatccc acgaaaggct tgtgaccaga tcaagatagc ggacgttgtg
     5341 tttgcggcaa tcggtttgga tggattgtct ccatgcctgc acgcgggcgc ggtaaagatt
     5401 tcgcaggcca ttgtctatgg aaacatcctg ctggaaattg gtttcgatat ccacaagctg
     5461 caaatcgcct gcgagcggcg gatctacttc gtcaggtgca aggatgtgaa tcatcgccgc
     5521 ttcatgtccc cgcccgagca gttggcgcag acctgattcg tatccatccg cggtgagcag
     5581 atcggaaatc agaatcacga gccctgcgcg gcgcggttga attgaataat cgcgcaggga
     5641 ggtattgaga tttgtcgttt cgcctgcctt gagcgactct aaaaatttga acattcgtgg
     5701 cagggcatat tgtccgcgtg agggaccgaa ttcactggca acgcgcccgc cttgcaaaaa
     5761 gccgaccgag agcaaatcac ctgaactcaa cgcgatcgcg ccagtccctg cggcgaggcg
     5821 caatgcgtac ttgaacttgt tttcttcctg ctcgccccaa tccatggagc gtgagccgtc
     5881 tatcaatatg taaacggcaa ggtcttcctc ctcttccagt aatttgatga atggtttttc
     5941 caaccgcgcg tagatgttcc agtccaaacg gcgcaagtca tcgcctgcgg tgtagttgcg
     6001 ataatcggca aattccacgc tgctgccgcg gcgcgaagaa cgccgctcgc ccttgatcgc
     6061 gcccgagcgc acacgcgagg ctacgagggt gagttggttt agcttgcgaa gagtggattc
     6121 gtcgaagagc ataaggataa aggatgaatg atgaaggatg aaaaaaaagt taaattttgc
     6181 ccattcgttt tttgattttg gttacgatgg tggtgaagat ggcgatgagt tcgttagttt
     6241 ctttgatgag agcttctgct ttttgggcgg ataggatgcc actccttacc aatagatcga
     6301 gccaataatc ggtttcgtct aattcttgta agacagactc aaatttgttg acaatatcag
     6361 agtcggattt ggcgcgatgt ccttcacggt aatttgcgcc aatagatgtg ccagaacgta
     6421 acacctgctt tccaagaacc tgtgcctctg ttgtttttgg caggctggaa taaagtttga
     6481 tgatccgcaa agcaaaatcg gacgttcgca ttcgcaaatc ttggggagtt ttactttcca
     6541 tctttgcctt ttttgttctt gatcttattc atccctcatc cttcatcttt catccttgca
     6601 tttacaagtt cttcgatcac atcatccgcc ttcacaccct ctgccatccc atcaaaattc
     6661 aacaacaacc gatgtcgcag cgccgccttt gccacggcat ggacatcttc aaatgccacg
     6721 ttgtatcgtc ctgcaagcaa cgcagtgacc tttgcgccga gcaccagtgc ctgtccgccg
     6781 cggggagaga ctccgtagcg gacgtatttt ttcacgagcg gcgaagcggc agagtcttcg
     6841 gggtgggtgg cgagcatgag tctcgcgatg tattctttga cgtgtgacgc gatcggtgtt
     6901 gtgcgggcga gcgcgcgcat ttggatgata tcggccccag tgacggcttt gcttgccgca
     6961 ggtgattcct tgcctgtggt gcggtctaaa atctcgacca tttcacccac gttgggcaaa
     7021 tccacattta ctttgaaaaa gaaacgatcc agttgcgctt cgggcagggg ataggtccct
     7081 tccatttcga gcggattttg tgtcgccatc acgaagaacg gttcttcgag tttgtaagtt
     7141 ctttgagcca ccgtcaccga ggcctcctgc attgcttcga gcaacgcaga ttgcgtcttc
     7201 ggtgtggcgc ggttgatttc atctgccaga accaaatttg agaacagcgg cccttcgcgg
     7261 aaacgaaatt cgcgcttgcc gttgtcgtcg ctgaggattt ctgttccgag aatgtcagcg
     7321 ggcatcaggt ctggcgtgaa ttggatgcgc gagaatttca aatccaatgc ctgcccaagc
     7381 gtgcggatca acatcgtttt gcccaaccct ggcacgcctt ccaaaaggac gtgtccgccc
     7441 gcgaggatgc tcaccagcac atgccgtacc acatcgccct gaccgacgat cacgcgcccc
     7501 acttcggttt cgattcggtt tgcatattcg cggaaggtgt caacagaaaa atcgctcatg
     7561 gtcgctccgt tatttcaaag agataaaata atttcggatc aagtcaaaga attgcatggg
     7621 cacttgtcca ttttcgatgg cttgataata gaactgctcg tattgcgtaa acacattggt
     7681 gtacggcaca aggctttgac catccgtcgc ggtggtgggg ctggtgccga ccacatcgcc
     7741 gtcatttcca gaagtgggaa gcccaacgtt ctctccgttt tcgccgccaa gcaacgatgg
     7801 agcatagatc tgttcgtatg tggattcgcc gccatcgcct gcgccgttat tttgctcgat
     7861 gggagaactg cccgcctcat ttccctgttg aggggaatct ggcgaatcac ccgaccccga
     7921 gcctgatcca gcgctgctat tttgtccacc cgaattattt ccctgctgcc cctgaccctg
     7981 accatttgcc tgcgccgatt gattcccgcc gcccgccgcc aatagctgac ctgcaccctg
     8041 ttgcatttgg cccgccgcct gactcgcagt ctgcgcggca gtgacttgct gacctgcatt
     8101 cgccatttgt tgcgaggcat tggcaagcgc ctgctgtgcc gcggccaaat ccccattgcg
     8161 gatcgcgtct gcggcttgtt gcatggcctg tgccaattgc gggtttgtgg atgccagtga
     8221 ttgtgccgtg gcttccagtt ggtcggcgag tttttgcaac tcttcagggc tcatctgact
     8281 caagtccatg ttgttgagtt cattcgcggc ttgtgtgaaa tttccacttg caaggttttg
     8341 ccccaccgat gaaagcggag tcccttcttg tgcttgcagg gaactgcccg cctctttcaa
     8401 cgcctgtgcg gtttgacctg cctgctcact attcagggct tgcagttgtt cgcctgtatt
     8461 cgtcaaaatg gaaacagcac cttcgagcga tgggttctgg tcgagggctt gcaaggcctg
     8521 gttgagcggt tcggtcagtt cctgtttttg ttcggcggtc agcgcttcgt tttcgttgat
     8581 ctctttgatg atctcttcga tctgttcctg ttgtgcggcg atggcctgct ccacagcgcg
     8641 ctgacggctt gcagcggcga acagcgcatc accgcggaac cataaaacac cgatcaacag
     8701 cgcgaggatc aaggccaggg ctgagtcgaa tctttttagt cgcagaggca ggtctcggcg
     8761 gggttggatg cggtgcgcgg cggagaccgc gtcgtccaat tgcttttgaa ccatttcgtt
     8821 ggcgtggtcc gcggtgctta gttccagcgc agtgctgatg cgttcttcga gatgaaaggc
     8881 gcggtcaaaa tgacgcgcgg cttttgttgt atttactttc cagaaaaatg cgatgatcgc
     8941 tgagaggatc ggtaataaag tgaccgaggc aacgacaagc gcgagaaatt cctgtttcaa
     9001 gagttttgcc tgagccagcc cgatgccgcc aaagagcagg gagagcccca gcgctgcggc
     9061 caacccgcgc agataccagg tcagtgcgcg ctggacgcgt aaccattgaa tccagttttg
     9121 gagggtgtga ttgagatgat tgatcgtgct catggtcgtt tgctccatta aggctcgtga
     9181 cgcttcaggt aaaagacgat taaccttatc attaaaatgg tcattgttat agagaagatc
     9241 gagtacaaaa tccatggtga caccaggacc atcggcggta cgggtgattg gatcaggaat
     9301 aaattttgtt cttcgatcag catggcttcg ctgacgaagg cggcagaagc tgggttggtt
     9361 gaaatgatgg tccacaacac gttgagcagc cagcgttgaa actcgaagct ggcatttgag
     9421 agcaggtagg aattttcatt tatggcataa atgcccgccg cgaataagag gatcggcagg
     9481 atgagggtgg tgtaggacag gatggttgcg atggatgtcc gtttggagat gcctgaaaag
     9541 tacaagccca gtgtgcagaa gttgatcgtg gaaagggtga gaatccagat ggaaattaga
     9601 acttcctcaa tgcccacgcc gcccagaaag aaagcaaggc tttgcagggg aattgcagcg
     9661 acaatgagca agagcaggta catgacggat gaccccagtt tgccgatcac aatgtctttt
     9721 gcgctgagca gtgacattcg cagctggtcg taggtctgcc gctctcgttc tgacgaaatt
     9781 gcaccagagg tgagcgcagg gccgatgaat cccgtcagga tgatttccag aagcacaacg
     9841 atcaaaaaga cctgtttgcc tgcctcctgt aaaaagccag gactgcggct gtaggtgttg
     9901 cgtactgaat agacatagac ggcgtaaatg acgaggctga tcagcccgag gtaggcggtc
     9961 agcatcacaa atccctgcct gccgcgcatg cggcctttta gttccttggc gataatgggg
    10021 ttgttcatcc agctccagcg gggtgctttt tcatgcaaag atgacgttga gccttccatg
    10081 ttggttcctt ttggcaatcg gctgctgagt gtaacaataa cattttacct gctctgcgtt
    10141 tttcgtttac aatccctgta tgcctctcgc acttgtcact ggcggtgccc acagactcgg
    10201 caaagccttt gccttatccc tggcccgcat gggatattcc atcgcgctgc attatcgtgc
    10261 ctctgccaat gaagccgacc acacggtcaa ggagattcgc gctctgggtg tggattgcct
    10321 cccgatccgt gcagatttga ccctgcctga aaaaatcgat ttcctttttt cactggtgga
    10381 tgaattcaaa accccactga aggtgctggt caattcggca gcagttatgc ccgttggaaa
    10441 accacaggaa ctcgagatcc atgattggga tttggcgctc gacctcaacc tgcgggcgcc
    10501 atttatgctg gcgcaggaag cggcaaagcg gatgaccgat ggcgggctga tcgtcaacat
    10561 tacagacatt ggcgcccaaa aggcgtggag ccgctaccca tcatacacgg ttagcaaggc
    10621 tgggctcgag tcgcttacga agctgctcgc tcgcgccctt gcgccgacga tccgagtcaa
    10681 cgccattgcg cctggtctcg tcctgccatc ggatgtggtc accgaagagc aatgggataa
    10741 cctcgttcaa aaacttccgc tcaagcgcgc cgccacgctc gacgaagtca cctccgcgct
    10801 tgaattcctc atcaaaaatg aatacatcac aggtcaaacc atcgttgtgg atggcgggta
    10861 ttcgttgctg tagtgtataa atggtaaaca aatataaaaa actgataact gctcactgat
    10921 aactattcac tgattgccaa tgtctttcaa aaaacttccc ctcgatcttc ttgcttcgat
    10981 cttcctgatt gctttcacgc tgttttatgc tgcacgcttt gtggattttt ccattccacc
    11041 ttttgaagat gccgccatgc tcatgcgtta cgcccagcat cttgcagaag gtcacggcat
    11101 tgtctggaat atcggcgaag cgcccgtaga tggcgcaacc gattttcttt tcatggccgc
    11161 ctctgctgct ttgatcaaat tgggttttac cgtcggtcag tctgtgcggg gcattggttt
    11221 tgtttcgcat ttgctcactg tcctcatcgt ttactggacg aatcgccgcg ttaatcacac
    11281 agatattccc ttttcattat taagcggact ttaccttgcc gttgggactg gattttccta
    11341 tgtggccgcc tacttcggga cgcctttctt tgccctcgcc gccgcctcca cctggaccct
    11401 gggcctgctc ctgattcaaa aagagaatcc acccttcagc ttgattctcg ccttcgccct
    11461 gagtggactt gtcacagggt tgatccgccc cgaaggggtc atccttgctt cgttgatgct
    11521 gctggcgatc atcgtcatgc gcggatggaa aaattcggtt gcgatcatcg tcacatttgg
    11581 gagtgttttc ctcttgttgg gtggggctta ctttgtgtgg cgctggaatt atttcggtta
    11641 cccgcttcca aatccatttt acaaaaaagg cgatagcggc ctgcactggg actcgttcta
    11701 caattccctg tctaacacat tgaggatgtg cctgccgttc gtgtttgtgt tcatcctggg
    11761 gtttcgctcc cgcgaaacct caacacggac gctcgcatac ctcattccga ttctcggctt
    11821 tgcgtctgcg tttatcttga tctccgacga aatgaacttc ggtgcgcgct ttcaatatgc
    11881 gcttgttccg ctcgtgctca tgtcgtggac tccgcttttg ttttcgggaa accagtcagg
    11941 gctgggtgtt ctttccagcc tgtatcgcga agtccctgta gggggagacc agaatcaact
    12001 gtcggttagg aatggacgcg tttatctttt tgcgggaatt gcgctgtttg tgagtctcgt
    12061 ctattattcg tcgtatcaaa attgcttcct cacatccttc cagcagacgt gtgcaaggcc
    12121 gtatgaacgc gatgggcgct atgagatggg cagattgttg ggagagtatc gcggcaaagg
    12181 ctatgtgatc gccaccacgg aggcggggtt ggtcccgtat tattctggct ggaccgcgat
    12241 cgatacctgg ggtttgaacg accaatttat agctcacaat ggaaagatca cagttgaata
    12301 tttggacaag tacaaaccgc acatcatcat gttccatgat tattattcgc cgcttgtgcc
    12361 gccaaagttg accgatgcca accttgccca gcgttggttc ggcatgacga tattgctcaa
    12421 gacttacgcc gaggaaaatg gatatatcct cgccgccgtt tttggtgaca gcccgtatga
    12481 cacaaactat ttttatgtcc gccctgattt tgaagacagc aaaaagctcg ttggtcagat
    12541 ttcgggtttc aaagattatt actggttcac cacaggaaaa cgctcgatca attacgcgga
    12601 atttgacaaa ccataaatta ccaattaccg aaggagtttt tcaatgtctg aaacaaccga
    12661 attggctgag aagttgaaaa ccgaaggaga aaaatatatt gagtttttct ctgggatgaa
    12721 cgaatcgctg tggcagcagg aggtatacac cgaaggcgaa atgtggaccg ttcgcaatgt
    12781 gctgtctcat tttgtcacct ctgagcgcgg actggtcaaa cttttcgagc agattcgtca
    12841 gggtggcaca ggcgccgcgg atgatttttc aattgaccgc tacaacgcca gtcagcaggc
    12901 aaagacgaaa gacctatctc cctccgagtt gttggaacaa tacaaagaag tgcgcgccaa
    12961 ctctgtaaaa tgggtctctg gcttgaaaga tgaagaactg gaaattaagg gccgccatcc
    13021 atttttaggg gaaactgttc ttcgcgaaat ggtgaaaatg ctgtacatcc acaatcaact
    13081 tcatttccgt gatttgaaaa aagcattgaa gtaaatgaca aatatgtgga tggtaatgtc
    13141 tcagccattc atccactctt tactgaccac tgaccactga ccactgacca ctgaccactg
    13201 accactgacc actgaccagg agcccaatca tgatacttcc ccccttcaaa ctcgaacgct
    13261 atttcgccaa atacgaattc accaccgaat atttactctg ctcttcagat tgtgaagcca
    13321 tgtccattgc agaattgctg gccttggaac caggctcggc tgagaaattc caaaatgtct
    13381 ggctggggta cacggaatcg ttgggcagtc catcgctgcg caaagagatc accaatctat
    13441 acaccactat gcagcccgat gatattttgg tccatacagg ggcgggggaa gcgatttatc
    13501 tttttatgca cgccgccttc gaaaaagggg accatgtcat tgtgcattcc ccaggctatc
    13561 aatctcttac ggaagtggct cgcgccgtgg gctgtgacgt cagcccgtgg cgagcaaggg
    13621 aacaggatgg ctgggctttg gatctggatg aactccgtca cctgatgcgg acaaatacca
    13681 aaggcatcat tatcaacacg ccgcacaacc caactggata cttaatgtcc catgcggatt
    13741 ttgaagcggt gaataaattc gcccaggaaa aaggcctgat ccttttctcc gatgaggtct
    13801 accgtgaatc tgaatacgat tccgccgata gactccccgc cgcttgtgac atgggtgaac
    13861 atgccgtgtc gctgggtgtg acctcgaaaa cctatggcct tgcggggttg cgcattggct
    13921 gggtcgctac aaagaacaaa aagatccttc aagccatggc ctcgctcaaa gactacacaa
    13981 ccatctgtaa ttccgcgccg agcgaattcc tagcggaagt tgccatgcgc aaccgccaaa
    14041 tattgatcga tcgcagtctc gggatcatca aacacaacct gactgtcgta gatgacctgt
    14101 tcaaccgcca tgctgcactt ttcacttggg cacgtcccaa agctgggtca atgggttttc
    14161 cgaaattgct caaaggtgat atcgaagatt tttgcgatga cctcgtaaga aaggcgggcg
    14221 ttttgctttt gccagggtct gtgtacgatg attccagaaa tcatttcagg ctggggctgg
    14281 gcaggaagaa ccttccgcag gcggtggaga ggttgga
//
LOCUS       DAOQJC010000105        14017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2102_length_14017_cov_10.882109, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000105 DAOQJC010000000
VERSION     DAOQJC010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14017
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2102_length_14017_cov_10.882109"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2363)
                     /locus_tag="PLE39_14400"
     CDS             complement(<1..2363)
                     /locus_tag="PLE39_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69572.1"
                     /translation="MRDWSLRPGDPLYLTLAADARLCKPDYVNDHIWEVELGATDPER
                     AAISLYTTFGLRARSMRLSLRFTENNVAVTDPNTFFTKPSLRRFFPNFLTLDFVPLEN
                     LQVSTEFWVPESHAVAGRVILTNKSNAVRQIKLEVCAALAPLDGQSIIPTQQQLVNIL
                     AGQTSGIAPVIFMTGGPKHGPGPQPSLLLELELGPGATRQITFAEAALDTVAMSFELA
                     RHSAARPWEAERARIELLDASQVLDIRTGDPDWDAALAFSQRASHGLFFSGNGGLPNA
                     SFVNARHVDNGFSRKGDGADYPPSWNGQSPLEAYYVSSILQGTPHIAKNLLLNFLATQ
                     DEDGEVDNKPGITAQRGKLLAAPLLASLTWKYYQATQDEAFLAEAFPKLIKFFWSWFS
                     SAHDRNRDGAPEWDHILQTGFDDNPVFDVWHPWSQGLDISLVHSPSLEAMLYREAWSI
                     VKIAKRLGKPEEETALVIAQSEILKTSINASWNPNQSFYSYRDRETGLVSTGQVIAKK
                     KGNGNARPKIEFETGVRLLVEIQTKNPAAKRPEVEISEYFTKSKGEVETILGHQFQWR
                     TGGLVATTQKLFTRVGRVSVTGLDEKDKVLVKLIDTTGEDLTLALPLWAGVPEAQQVN
                     ALIGRNLLTADRFDRPFGFPSLAASPNPEADTVSMSVTLPWNQLIGEGLLAYGFRAEA
                     TRLTAHLMNGVIQNLKQNRSFYNRYHAEKGTGIGERNSLHGFAPVGLFMQTLGVTILS
                     PTKVKLEGKNLFPWAVTIKYKNLTISRGLEQTVVTFPNGESVIVKEET"
     gene            2468..2917
                     /locus_tag="PLE39_14405"
     CDS             2468..2917
                     /locus_tag="PLE39_14405"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HNE69573.1"
                     /translation="MAGLYFEEFSVGQKFNTVGRTVSEGDIFNFAGLTGDFNQIHTDA
                     AFAAKTQFGQRIAHGLLGLSLATGLIMRTGLLEGTVLAFREINEWKFVKPFYIGDTIY
                     AELTVTETKALPRIGGGALISAIAVKNQSDEICQRGSLNLLVLSKPK"
     gene            3069..6104
                     /locus_tag="PLE39_14410"
     CDS             3069..6104
                     /locus_tag="PLE39_14410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNE69574.1"
                     /translation="MNPPSGNPSQTGNTTPLYRPALDKDNMPLPRRVTEVDLEGTRVS
                     RAAYEPTSQRPPQQTPPPQIPRQNNPQFNFPPIDWRKGWGCFLRGLIASAFVLVIVGL
                     CLGSYFVYQYYSIASTLPDVGDLRDRASQFETTRILDRNGQVLYEILDPSAGRRNYIP
                     LSEISPNLVAATIATEDKEFYTNPGFDPIAIIRALWQNYVTGGQGGGASTITQQLARA
                     LILSPEERTQRTVTRKAREIILAAEITRRFTKDEIIELYLNEIYYGNLAYGVEAAAET
                     YFGKSAKDLTLAEASFLAGLPQSPAVYDIYTNPEATLIRQQQVLVLMFEQSTDQECIK
                     VSNSETPICVDPIAATQAADAMKTYQFRVPSFDAKYPHWVNFVRSQLEEQYDAQTIYR
                     SGFVVYTTLDATMQDKAQEFVSGQISNLVDNNTHNGALVAIRPSTGEILAMVGSPDFN
                     NAAISGQINMAVSPTRQPGSSIKPFTYVAAFEKGWTPSTLIWDVPTQFPDGANPPYEP
                     RNYDGKFHGAMTVRIALSNSFNIPAVKALEFVGIYDDPNTPQKDGLIGMAERLGITSL
                     TRNDYGLSLTLGGGDVSLIDMTSAYSVFANSGKRMPPVAILKITDYAGNLIYEYKPAE
                     GEQVIRSEHAYLISSILSDNESRSLMFGPNSQLNLSFPVAAKTGTTNDIRDNWTIGYT
                     PDLVTGVWIGNADYTPMINSTGLSGAAPIWHNFMEFAVPYLTNGAPNWFTRPSGIMDR
                     IVCKLSGTEPGNMCGAQYTEVFASDQPPLQPGQDLVRRIRIDLWTGLQSSDACNGPKD
                     EAVVMNVTDKWARDWLATNPGRDWLENHDLPRNPMYAPERECSANDPQPIIEIDLSDG
                     ETVTSPILNVKGSAAANGGFQGWTLDYGQGAEPGSWSVLTASNTPIKDGSLYLWDMST
                     LPNGIYTLRLTLIGERAEVDDRVTLSLNLPIPTVQVPTLTATPVPTIFIIPTIPTRTP
                     TPVIIIVPPTSTPIPTETPTEFPTETPTETPTESPTPGP"
     gene            6315..10562
                     /gene="rpoC"
                     /locus_tag="PLE39_14415"
     CDS             6315..10562
                     /gene="rpoC"
                     /locus_tag="PLE39_14415"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNE69575.1"
                     /translation="METKGLTALRISLASPQTIMSWSYGEVLKPETINYRRLRPEKDG
                     LFCEAIFGPTRDWQCYCGKYKNPRYKGITCEKCGVEVTRSAVRRERMGHITLATPVAH
                     IWYTRRIPSQMGMLLDVSRRNLDRVLYFAQYVVTYVDEEARKKALQRLEDEITVSERE
                     QAAGVNSKIAEIKTKRDQKIAEIKQKQTALEQGYDEGIAEQMDPIIKEGQKLEKLLQS
                     QMGEPAKKAVVFELTGEKIIDANDKVASKHITMIQKSVQKRLESLENELKDKKQREVE
                     ELKTQAAAIKAQADQEMEALRSALENETTDSSNSSSRLRDELLELRPFTFLSEIRYRE
                     LKQRWGQVFRADMGAEAFFDILERLDLDKLSEELWHEVRTTKSKQKRKKSTTRLKVVE
                     AFRRSGNSPAWMILTVLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLL
                     ELGAPDVIIRNEKRMLQEAVDSLIDNSQRGKALSRRGRRELKSLSDMLKGKKGRFRRN
                     LLGKRVDYSGRSVIVVGPQLKLNQCGLPKSMALELYRPFVIARLVQNNYAANVKGARR
                     LIERNRPEVWEALESVIGDRPVMLNRAPTLHRLGIQAFEPILIEGSAIQLHPLVTTAF
                     NADFDGDQMAVHVPLSQKAVAEARELMLASRNLLKPADGEPIISPSKDMVLGVYYLTM
                     EKKAVHRGDGRAFADMDEVELAYGLGQVEIHTEIKFRAKTWYDDKGDRLAEPEVRVLN
                     TTVGRVLFNRVLPEQVQFMNEKLDKGGVKDLIAEVYELCGQEVTTHVADAVKSIGFEY
                     ATRSGTTLAVADISIPPERKGIIEEALKSVEVVMRDFRRGLLTEQEKNEREIQIWQEI
                     TERVGVAVKKHMDPDGNLSIMATSGATKGGFSTISQLAGMRGLMADPSGRIIPMPIRS
                     NFREGLTSQEYFISTHGARKGLADTALRTADAGYLTRRLVDIAQDIIINEHDCGTHDG
                     IWIRKSDDVAGQSITSRIYSRLVAERILDPKTGEVIAEYNDVITHDLARKIAGAGVTE
                     VKLRSPMTCQLSHGICSKCYGIDLARGELVDLGAAVGIVAAQSIGEPGTQLTLRTFHT
                     GGVASQGASDITTGLPRVEEIFEARKMPKGEAVVAEISGMIHIRQSEKYADMREVRIE
                     HAEMVHDEYVIPEDWKFVAKDETEVQAGDVLANKEKATIIAQHAGRVAVEKKDHKIIV
                     SYEQREEVIEDIPTTSRLLVKDGAHVDAGQPLTEGSLNPHRVLKIQGRDACQMYLMTE
                     VQKVYRSQGQNIHDKHFEVIIRKMLSKVQVTRPGDTKYLPGDAVDRLEIRKINEALVA
                     EGKQPARFQEILLGVTKASLSTDSFLSASSFQHTIKVLASAAIGSTTDPLFGLKENVI
                     IGKLIPAGTGFIHGRFADAESTQNATQWSNMPDMGSI"
     gene            10805..12136
                     /gene="ablA"
                     /locus_tag="PLE39_14420"
     CDS             10805..12136
                     /gene="ablA"
                     /locus_tag="PLE39_14420"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HNE69576.1"
                     /translation="MPTPIVSKRAPIYADVPDEKWNDWRWQLSHRLNTAEDIEKVIPL
                     TDSERKALQTQGLFRVDVTPYFISLIDPNDPNDPTRKQIIPVSEELNAFTAMMEDSLA
                     EDRHSPVPGLVHRYPDRVLMLVTTQCASYCRYCTRSRIVGDPGQTFNRQEFEMQIEYL
                     KKTPQVRDVLLSGGDPLVLAPKVLEELLTRLREIPHIEIVRIGSRVPVFMPMRITQEL
                     CDMLAKFHPLWLNIHVNHPNEISLELAQACDRLTRAGIPLGNQSVLLAGVNDCAHLQR
                     ELVQKLTRIRVRPYYLYQCDLVEGAGHFRTPVAKGIEIIEALRGHTSGYAVPQFIVDA
                     PGGGGKIPVMPNYMLSMSDHKVIVRNYEGYVTTYEEPTEYKSHDPKTCKFCQSKRPEL
                     GQTGLTGLLDGEELFIKPEGFDELHNRGGGQHRLRDENKWKPLGIGSGETD"
     gene            12170..12817
                     /locus_tag="PLE39_14425"
     CDS             12170..12817
                     /locus_tag="PLE39_14425"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNE69577.1"
                     /translation="MKKMNRAMITLLVLALSLSACLPATASNQQAQQVQNTQQSSQDV
                     QSNIETAVAQTVEAQNEISTSVAQTMQAQATLTPTPTATLFSLPTVTPFVVSTPTVRP
                     AGGGGAPVKAEYSCDIIHQRPYDNQEFLHGQEFDIKWTILNTGTKTWPAGYDLKYWSG
                     PKMTTAGTVQLPSMAPGDQFDVVFDAVAPDEPGFQVMTWVVEGQICFPYIAIFVK"
     gene            12837..13466
                     /locus_tag="PLE39_14430"
     CDS             12837..13466
                     /locus_tag="PLE39_14430"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNE69578.1"
                     /translation="MLKRNQRIQAILFATVLMLACAPLATPYAPPTFDLLQVNTIIAE
                     TAGAAATQTFVLLPTSTVTPTFTRAPSETPSPTNTFIFVLATPTVPSLTPTPEKSDEP
                     YDCQVLSQNPVDDTSFVPGLAFQTRWVVKNIGTETWGVNTADYRFVSGTKFHKLSVYD
                     MPTSIPPGEKVEIFVEMWAPMDPGTYTTTWRIKVGKTEFCSMKLKIIVP"
     gene            complement(13517..13816)
                     /locus_tag="PLE39_14435"
     CDS             complement(13517..13816)
                     /locus_tag="PLE39_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69579.1"
                     /translation="MNNKNTGMIATIATALICGCCALFNCAMSIGTFSGNGSFSLGNN
                     SQPMPPAMGAVFLCLSFIMILIPVAVGFFTLRKKPEAVAVVEETPAKDEPLPPAS"
BASE COUNT         3310 a         3752 c         3651 g         3304 t
ORIGIN      
        1 gtttcttctt ttacgatcac gctttcccca ttggggaagg ttaccaccgt ttgctccagt
       61 ccacgcgaga tggtgagatt tttatatttg atcgtcactg cccaggggaa aagatttttg
      121 ccttcgagtt ttacttttgt aggagatagg atcgtcacac ccaatgtttg catgaataac
      181 cccaccggtg cgaagccatg cagtgaattg cgttcaccga tgcctgtccc tttttcggcg
      241 tggtaacggt tgtagaaaga ccgattctgt ttcaggtttt ggatgacacc gttcatcagg
      301 tgtgcggtga ggcgggtggc ttcagcgcgg aatccgtatg cgagcaagcc ttcgccaatc
      361 aattgattcc aagggagagt cacgctcatc gaaaccgtat ccgcttccgg gttgggcgaa
      421 gcggccagtg acgggaatcc aaatgggcgg tcgaagcggt ctgctgtcaa caggttgcgc
      481 ccgatcaagg cattcacttg ttgtgcttca ggcacaccgg cccagagcgg cagtgccagg
      541 gtgaggtctt cgccggtggt gtcgatcagt tttacaagca ccttgtcttt ttcatcgagg
      601 ccggtcacgc tcacgcggcc tacgcgtgtg aataattttt gcgtggtagc caccagccca
      661 cccgtgcgcc attggaattg atgccctaaa atggtttcca cttcgccttt ggattttgtg
      721 aagtattcgc tgatttccac ttcggggcgc ttcgcggctg gatttttggt ctgaatctcc
      781 accaacaagc ggaccccagt ttcaaactcg atcttggggc gggcattgcc atttcccttt
      841 ttcttggcga tgacctgacc tgttgagacc agccccgttt cgcggtcacg ataggaatag
      901 aaactctgat tcgggttcca acttgcattg atggatgttt tgagaatctc agattgcgca
      961 atgacgaggg cagtttcttc ctcgggtttg ccaaggcgtt tggcgatctt cacaatggac
     1021 catgcttcgc ggtacagcat ggcttcaagt gacgggctgt gaaccagtga aatatccaat
     1081 ccctgcgacc aggggtgcca aacatcaaaa acagggttgt catcgaaacc ggtttgcaga
     1141 atgtgatccc actcaggggc gccatcacgg ttgcggtcat gtgcggagga gaaccatgac
     1201 cagaagaatt taattaattt ggggaatgct tcggcaagga aggcttcatc ctgcgtggct
     1261 tgataatatt tccacgtcag gctggcgagc aggggggcgg cgaggagttt tccgcgctgg
     1321 gcggtgatgc cgggtttatt atccacttcg ccgtcttcat cctgtgtggc aaggaaattg
     1381 agcagaagat ttttggcgat gtggggtgtg ccctgcagaa tactggaaac gtagtacgcc
     1441 tccagcggtg attgtccatt ccatgagggt gggtaatctg cgccatcgcc tttgcgtgag
     1501 aagccgttat ctacatggcg ggcgttgaca aaagatgcgt ttggcaggcc gccgttgccg
     1561 ctgaagaaca atccatgcga ggctctttgg ctgaaggcta gcgcggcgtc ccagtcaggg
     1621 tcaccggtgc ggatatctaa aacctgtgag gcgtcgagca attcgatgcg ggcgcgttct
     1681 gcctcccacg ggcgggcggc gctgtggcgg gccagttcaa aagacatggc gacggtgtcg
     1741 agcgctgctt cggcgaaggt gatttgccgc gtggcaccgg gaccaagttc cagttccagc
     1801 aggagcgagg gttgcgggcc ggggccatgt ttggggccgc cagtcataaa gatgacgggg
     1861 gcgattccgc tggtttgtcc tgccagaatg ttgacgagtt gttgttgggt ggggatgatg
     1921 gattggccgt cgaggggggc aagtgcggca cagacttcga gcttgatctg gcgcacggca
     1981 ttgcttttat tggtgagaat cacccgcccg gccacggcat gtgattcggg tacccagaat
     2041 tcggttgaaa cttgcaggtt ttcgagggga acaaaatcga gggttaggaa gttagggaag
     2101 aaacggcgca gcgacggttt ggtaaaaaag gtgttagggt ctgtaactgc gacattattt
     2161 tcggtaaaac gcaatgaaag gcgcatggag cgggcgcgta aaccgaacgt ggtatagaga
     2221 gaaatggcgg ctcgctcggg gtctgtggct ccgagttcaa cctcccaaat gtggtcgttg
     2281 acgtagtctg gtttgcacag gcgggcgtcc gctgccagag tgaggtaaag cgggtcgcca
     2341 gggcgtagag accaatcgcg catagggcaa ttgtacgggc gaaatggtgt gaggtcaaac
     2401 gggtataatc gttacaacgt tgacacgttc aaaggttgta acgttaaaaa agacgaggag
     2461 actaaaaatg gctggacttt atttcgagga attttctgtc gggcaaaaat ttaatacggt
     2521 cgggcgcacg gtcagtgaag gtgatatttt caattttgcc gggcttacag gcgatttcaa
     2581 ccagatccat acggatgcgg cctttgcggc gaagactcaa ttcggtcagc gcatcgctca
     2641 tggacttttg ggcctgtcac tcgccaccgg cttgatcatg cggacgggat tgctggaagg
     2701 caccgttctc gctttccgcg agatcaacga gtggaagttc gtcaagcctt tttacattgg
     2761 cgatacgatc tacgctgaat tgaccgtcac cgaaaccaaa gccctgccgc gcatcggcgg
     2821 cggcgcattg atctcggcca ttgctgtgaa gaatcaaagc gatgaaattt gccagcgtgg
     2881 ttcgttaaat ttgctggtgt tgagtaagcc aaaatagttt ttattaacca gtctccaatt
     2941 atcttttatg gaattcataa ttgataattg gagatttatg ggccaacctg atgaacgaag
     3001 acaacgatcg attacgccga attataaaat cagaagaaga aacacggggg gaacacaccc
     3061 ctgcgccttt gaacccacct tctggaaatc cctctcaaac tggaaatacg accccgctct
     3121 accgccccgc attagacaag gacaatatgc ccttgccgcg ccgagtcacc gaggtggatt
     3181 tggaaggtac gcgagtcagc cgggccgctt atgaaccgac ttcacaacgc cccccgcaac
     3241 agacaccccc accgcaaatt ccccgccaga ataaccctca attcaacttt ccgccgattg
     3301 attggcgcaa aggttggggt tgttttctgc gcggtttgat cgcttcggct tttgtactgg
     3361 tcattgttgg tttgtgtttg ggttcgtact tcgtttatca atattattcc atcgccagca
     3421 cccttcccga tgtaggtgat ttacgcgacc gggcttcgca gtttgaaacc acacgcattt
     3481 tggatcgcaa cgggcaggtg ttgtacgaga tcctcgaccc cagtgccggg cgccgtaatt
     3541 acattccgct ttctgaaatt tcacccaatc ttgtcgcggc aaccattgcc accgaagata
     3601 aagagttcta caccaacccc gggtttgacc ccatcgccat tatccgcgct ttgtggcaaa
     3661 actatgtcac aggcgggcag ggcggcggtg cctccaccat tacccagcaa ttggcgcggg
     3721 cgttgatcct ctctcctgaa gaacgcaccc agcgtacggt cacacgcaag gctcgtgaaa
     3781 tcatcctggc agcagaaatc acccgccgct tcaccaagga tgagatcatt gaactgtatt
     3841 tgaacgagat ctattacggc aaccttgcct atggtgtcga agccgctgct gaaacctact
     3901 ttggcaaatc ggccaaagac ctcaccctgg ccgaggcttc cttccttgcc gggctgccgc
     3961 aatctcccgc tgtgtacgat atttacacca accccgaagc caccctgatc cgtcaacagc
     4021 aggtgttggt actcatgttc gagcaaagca ccgaccagga gtgtattaag gtcagcaaca
     4081 gcgaaacacc gatctgtgtg gatccgattg cagcgacaca agccgcagat gcgatgaaga
     4141 cataccaatt ccgtgtgcct tcgttcgatg caaaatatcc gcattgggtg aattttgtcc
     4201 gttcccagct ggaagagcag tacgatgcgc agaccattta tcgctctggc tttgtggtgt
     4261 acaccactct ggatgccacc atgcaggaca aagcgcagga atttgtctcc gggcagattt
     4321 ccaatctggt ggataacaac acccataacg gcgcacttgt tgccattcgc ccatctacgg
     4381 gcgagattct ggcgatggtt ggctcacccg atttcaataa tgccgccatc tccgggcaga
     4441 tcaacatggc tgtcagtcct acccgccaac ccggttcatc catcaagccg tttacctatg
     4501 ttgctgcttt cgaaaaaggt tggacaccct ccacgctgat ctgggatgtg cccacgcaat
     4561 ttcccgatgg ggcgaatccg ccttacgaac ctcgtaacta cgacggaaaa ttccacggtg
     4621 ccatgaccgt acgtattgca ctttcgaatt ctttcaatat ccccgcggtc aaggctctgg
     4681 agtttgttgg catctatgac gatccaaata ccccgcaaaa agacggtttg atcggcatgg
     4741 ccgaaagatt gggaatcacg tcgctcacac gcaatgacta tggcttgtct ctcacgctgg
     4801 gtggggggga tgtcagcctg attgacatga cttccgcgta ctctgtgttt gccaacagtg
     4861 gaaaacgaat gccccccgtc gccattctta agatcaccga ttatgccggc aaccttattt
     4921 acgagtacaa acctgccgaa ggagagcagg ttattcgctc agagcatgcc tacctgattt
     4981 cctccattct ctctgataat gaatcccgct cgttgatgtt tggtccaaac tcgcagctaa
     5041 atctgtcctt ccccgttgcc gcgaagacag gcaccaccaa cgatatccgc gataactgga
     5101 ccattggcta taccccagac cttgtcaccg gtgtgtggat cgggaatgcc gattacaccc
     5161 ccatgatcaa ctccacgggg ttgagcggag cagcgcccat ttggcacaac tttatggaat
     5221 ttgcggtccc gtaccttacc aacggtgcgc caaactggtt cactcgtcca tctggcatta
     5281 tggatcgaat cgtctgcaaa ctttcaggca ccgaacccgg gaatatgtgt ggcgcacaat
     5341 atacagaggt ctttgcctca gaccagcccc ctctgcagcc tggtcaagat ctggttcgtc
     5401 gtatccgcat tgacctgtgg accggcttgc aatcttccga tgcctgtaac gggccgaaag
     5461 atgaagccgt ggtcatgaac gtcaccgata aatgggcacg tgactggctt gcaaccaacc
     5521 caggccgtga ctggcttgaa aatcacgacc tgccgcgcaa tcccatgtat gcccccgaaa
     5581 gggaatgcag tgcgaacgac ccgcagccca ttatcgaaat cgatctcagt gatggagaaa
     5641 ctgtcacctc acccattttg aatgtcaaag ggtctgctgc tgcaaacggt ggtttccaag
     5701 gttggacgct ggattatggt caaggagctg agccaggctc atggtccgtt ttgaccgcca
     5761 gcaatacccc catcaaagac ggctcactct atttgtggga tatgtccacg ctgccaaacg
     5821 gaatttacac gctgagacta accctcatcg gcgaaagagc ggaagtggat gaccgtgtca
     5881 ccttaagcct caacctgccc atccctacgg tgcaggtgcc tacgctgaca gcaactcctg
     5941 tccccaccat cttcatcatc cccaccatcc caacccgtac ccccacaccg gtcattatta
     6001 tcgtcccacc caccagcacg cccatcccta ccgaaacccc gaccgagttc cctaccgaga
     6061 cacccaccga aaccccaacc gaatccccca cacccgggcc ttaaagttgg agactcttca
     6121 ctgattaaca cccttgacga ggggttctcc cacgcatata atcacttcgt tggatgtaaa
     6181 aaccgagtca cacacagcaa gcgcttgaga gaatcctctc ggagcgcttg cctttgtttg
     6241 tctctgcagt aagttaacta cacgctttgc agagacgccc gaaaagaact ctatttattt
     6301 gaggagagta ctcagtggaa accaagggat taaccgcact ccgtatcagc cttgcctcac
     6361 cacagacaat catgtcttgg tcatacggcg aggttctcaa accggaaacc atcaactatc
     6421 gccgccttcg cccagagaaa gatgggttgt tctgtgaagc catctttggc cccacccgtg
     6481 attggcagtg ttactgtggc aaatacaaga acccccgcta caaaggcatt acatgtgaaa
     6541 aatgcggtgt tgaagtcacc cgctcagccg ttcgccgcga acgtatgggg catattaccc
     6601 tggccacgcc ggttgctcac atttggtata cccgccgcat cccttcgcag atgggtatgc
     6661 tgctcgatgt atcccgtcgt aacctggacc gtgttctgta tttcgcgcag tatgttgtca
     6721 catatgtgga cgaagaggcc cgcaaaaagg ccctccaacg cctcgaagat gaaattaccg
     6781 tttctgagcg cgaacaggct gccggtgtaa attccaagat tgctgaaatc aaaaccaagc
     6841 gcgatcaaaa gatcgcggag atcaagcaga agcagactgc gcttgaacaa ggttacgatg
     6901 aaggtattgc cgagcagatg gatcccatca tcaaggaagg ccagaagctt gaaaaattgc
     6961 ttcaatctca aatgggtgaa cctgccaaga aggcggttgt ctttgagttg accggtgaga
     7021 aaatcattga tgccaacgac aaagttgcca gcaagcacat caccatgatt caaaagagcg
     7081 tccagaagag actggaatct cttgaaaatg agttgaagga taagaagcag cgcgaagttg
     7141 aagaactcaa gacacaggcc gctgccatca aagcccaggc tgaccaggaa atggaagccc
     7201 tgcgcagtgc gctggaaaat gaaactactg attcttccaa cagttcctca cgcctgcgtg
     7261 atgaattgct ggaactgcgt ccatttacct tcctcagcga aattcgctat cgcgaactga
     7321 aacaacgctg gggacaggtc ttccgtgctg atatgggtgc cgaagccttc ttcgacattc
     7381 tcgaacgcct tgacctcgat aaactctctg aagaactttg gcatgaggtc cgcaccacca
     7441 agagcaagca gaagcgcaaa aaatctacaa cccgtttgaa ggtggttgaa gcattccgcc
     7501 gctctggtaa cagcccggcg tggatgattt tgaccgttct gcccgtcatt cctccggacc
     7561 tgcgcccgat ggttcagttg gacggcggcc gtttcgccac ctccgacctc aatgatctgt
     7621 accgccgtgt gatcaatcgt aacaatcgtt tgaagagatt gcttgaactc ggcgcaccgg
     7681 atgtcatcat ccgcaacgaa aagcgtatgt tgcaggaggc tgttgacagc ctgatcgata
     7741 acagccagcg cggtaaggcc ctcagccgcc gtgggcgccg tgaactcaaa tccttgagcg
     7801 atatgcttaa aggcaagaag ggtcgtttcc gccgcaacct gcttggtaaa cgtgtggatt
     7861 actccggccg ctccgtgatc gtggtaggtc cgcagttgaa gttgaaccag tgcggtctgc
     7921 ccaagagcat ggcccttgaa ctgtatcgcc cattcgtcat tgcacgcctt gtgcagaata
     7981 actacgcggc aaatgtcaaa ggtgcgcgtc gcttgattga gcgtaacagg cccgaggttt
     8041 gggaagcgct cgagagtgtc atcggtgacc gccccgttat gttgaatcgt gcgccgaccc
     8101 tgcaccgttt gggtattcag gcctttgagc cgatccttat cgaagggtct gcgatccaat
     8161 tgcatccgct cgtcactacc gctttcaatg cggactttga tggcgaccag atggctgttc
     8221 atgttccgct ttcacagaag gccgtggccg aagcccgtga attgatgctt gcttctcgaa
     8281 accttctgaa acccgctgac ggcgagccga tcatttcccc gtccaaggac atggtgctgg
     8341 gtgtgtatta cctcaccatg gaaaagaagg ctgttcaccg cggtgatggt cgcgcattcg
     8401 ccgatatgga cgaggtggaa ttggcctatg gcttgggtca ggttgaaatt cataccgaga
     8461 tcaaattccg cgcgaagacc tggtacgacg acaagggtga ccgtctggcc gaacctgaag
     8521 tccgtgtatt gaataccacg gttggtcgtg tgttgttcaa tcgcgttttg cctgaacagg
     8581 ttcagtttat gaacgaaaaa ctggacaaag gcggcgtgaa ggacctgatc gctgaagtat
     8641 atgagctttg tgggcaggaa gtcaccaccc atgtggccga tgctgtaaaa agtattggct
     8701 ttgagtatgc aacccgctcc ggtacaacgc tggcggtggc tgatatttcc attccgccgg
     8761 aacgcaaggg aattattgaa gaagccctta agtcggttga agtggtcatg cgtgatttcc
     8821 gccgtggttt gcttacagaa caggaaaaga acgaacgcga aatccagatc tggcaggaaa
     8881 tcactgaacg ggtgggtgtg gccgtcaaga agcacatgga tcccgatggc aacctttcca
     8941 tcatggcgac ctccggcgcg acaaaaggtg gtttctccac catttcccag cttgcaggta
     9001 tgcgtggttt gatggctgat ccttccggac gcatcattcc gatgccgatt cgttccaact
     9061 tccgcgaagg acttacctcg caggagtact tcatttcaac acacggcgcc cgcaagggtc
     9121 ttgccgatac tgctcttcgt acggcagatg caggttacct cactcgccgt ctggtggata
     9181 ttgcccaaga catcatcatc aatgaacatg actgcggcac ccatgatgga atatggatcc
     9241 gcaagtcgga tgacgttgcc ggtcagtcca tcaccagccg catttacagc cgccttgtcg
     9301 cagaacgcat ccttgacccg aagaccggtg aagtgatcgc tgagtacaac gacgttatta
     9361 cccatgacct tgctcgcaag attgccggcg ctggcgtgac ggaagtaaaa ctccgctcac
     9421 ccatgacctg tcagttgagc catggcattt gttcaaagtg ctacggtatt gaccttgccc
     9481 gtggcgaact ggtcgatctc ggcgcagcag tgggtattgt ggctgcccag tccattggtg
     9541 aaccaggcac ccagttgacc ctgcgcacct tccacacagg tggtgtggcc tcgcaaggcg
     9601 cttccgatat tacaaccggt ctcccgcgtg tggaggaaat cttcgaagcc cgcaagatgc
     9661 cgaaaggcga agctgttgtg gctgagatca gcggtatgat ccacatccgc cagtcggaaa
     9721 aatatgccga tatgcgcgaa gtacgtattg aacatgctga aatggttcat gatgaatacg
     9781 tcattcctga agactggaaa tttgttgcca aggatgaaac cgaggtgcaa gccggggatg
     9841 ttcttgccaa caaagaaaaa gcaaccatca ttgcccagca cgccgggcgc gtagccgtgg
     9901 aaaagaagga tcacaagatc atcgtttcct acgaacagcg cgaagaagtc attgaagata
     9961 ttcccaccac ctcccgcctg ctcgtaaagg acggtgcgca tgtggatgct ggccagccgc
    10021 tcacagaagg ctcactcaac ccacatcgtg tactcaagat ccagggtcgt gacgcctgcc
    10081 aaatgtacct catgaccgag gtccagaagg tgtatcgttc acagggtcag aacatccacg
    10141 acaagcactt cgaggtcatc atccgcaaga tgttgagcaa ggtgcaggtc acccgtcccg
    10201 gtgataccaa gtacctgccc ggtgatgctg tggaccgcct tgagatccgc aagattaacg
    10261 aggctttggt tgctgaaggc aagcagcccg cccgcttcca ggagattttg cttggtgtga
    10321 cgaaggcttc actcagcact gattccttcc tctcggcctc ctccttccag cataccatca
    10381 aggttctggc cagtgccgcc attggctcca ccaccgaccc gctctttggc ctcaaggaaa
    10441 acgtgatcat cggcaaactc atccctgccg gtaccggctt tatccatggc cgttttgccg
    10501 acgcggaaag cacccagaat gccacgcagt ggtcgaacat gccggatatg ggcagcattt
    10561 agctgttgtt tttgaaaaga cctgagcccg cccgcgaaaa gtttcgcggg cgggtctttt
    10621 ttaaagcaaa aatgggcctg ttgtaaaata ccgacattga cccactttcc gtttgctttt
    10681 acaatgttaa tcatgctttt gaagtttgca ggattctttc gcccggacca tgaccctcca
    10741 cacacggcag tgtgatctta gtcgtattgt cgtttaaaac ttattttaaa tttctggagg
    10801 aaaaatgccc acccctatcg tctcgaaacg tgccccaatc tatgccgatg tccctgatga
    10861 aaagtggaat gactggcgct ggcaattgag ccaccgcctg aacactgccg aggatattga
    10921 aaaagtcatt ccgcttaccg actcggaacg caaagccctg caaacccagg gattgttccg
    10981 tgtggatgtg actccctact ttatttcatt gattgacccc aacgacccga acgatcccac
    11041 ccgcaaacag atcatccctg tttcggaaga attgaatgcc tttaccgcca tgatggagga
    11101 ttcacttgcg gaggatcgcc actcacccgt ccccgggctg gttcatcggt atcctgacag
    11161 agtccttatg ctggtaacga cccaatgcgc ttcgtactgc cgttactgca cccgttcccg
    11221 catcgttgga gatcccggtc agaccttcaa ccgtcaggaa tttgaaatgc aaatcgaata
    11281 cctgaagaag actccccagg tgcgtgatgt gttgctttca ggcggtgatc cactcgtatt
    11341 ggcgccgaag gttctggaag aactgctcac ccgtttacgc gagattccgc atattgagat
    11401 cgttcgtatt ggctctcggg tgcctgtttt catgccgatg cgcattaccc aggaattatg
    11461 cgatatgctc gccaaattcc accccttgtg gcttaacatc catgtcaatc accccaatga
    11521 gatttcgctg gaacttgccc aggcttgtga tcgtctaacc cgtgcaggta ttccgcttgg
    11581 aaaccagtcc gtccttttgg caggcgtgaa cgattgtgcc catctgcagc gcgaactcgt
    11641 tcagaagctg acccgtatcc gtgttcgccc atactacctg tatcagtgtg accttgtaga
    11701 aggtgccggt cacttccgta cccctgttgc caagggtatc gagattattg aagccctgcg
    11761 tggacatacc tccggttacg ctgttccaca gttcatcgtg gatgcgcccg gcggtggtgg
    11821 taagattcct gtcatgccga actacatgct gagcatgtct gaccataagg tcattgtccg
    11881 caactacgaa ggctatgtca ccacctatga agagccgacc gaatacaagt cgcatgaccc
    11941 caagacctgc aaattctgcc agagcaagcg ccccgaactc gggcagactg ggctgacagg
    12001 cctgcttgac ggcgaagaat tgttcatcaa gcctgaaggt ttcgacgaat tgcataatcg
    12061 cggcggcggg cagcatcgtt tgcgtgacga aaacaaatgg aagccgcttg gcatcggttc
    12121 cggcgaaaca gactaaagga acccaaaaaa gtttgcgaag gagaaaataa tgaaaaaaat
    12181 gaatcgagcg atgattactc tgttggtgct ggcgttgtct ctcagtgcat gtctgcctgc
    12241 gaccgcatcg aaccagcagg ctcagcaggt acaaaataca cagcagagtt cgcaggatgt
    12301 gcaatcgaat attgaaacgg ctgttgccca gaccgttgag gcacagaacg agatcagcac
    12361 ttctgtagcg cagaccatgc aggcgcaggc cacgctcaca cccaccccca cggctacatt
    12421 gttttccctt cctactgtga cgccgttcgt tgtttcaaca cccactgtcc gcccggctgg
    12481 tggaggcggt gcccccgtga aagcagaata ctcctgcgat attattcacc agcgccccta
    12541 cgacaatcag gaatttctcc acggtcagga gtttgacatc aagtggacca ttcttaacac
    12601 cggcacaaag acctggcctg caggctacga tctcaaatac tggagcggcc ccaagatgac
    12661 cactgcaggg acggtgcagt taccctccat ggcccccggt gaccagtttg atgtggtctt
    12721 tgatgcggtt gcaccggatg aacccggctt ccaggtgatg acctgggtgg tggaaggcca
    12781 aatctgcttc ccctatattg cgattttcgt aaaataaaag ttctttggag gatttcatgc
    12841 taaagaggaa ccagcgaata caggcgatct tatttgccac tgtattaatg cttgcctgtg
    12901 ccccactggc gactccttac gcccctccaa cctttgacct tttgcaggtt aacacgatta
    12961 ttgcggaaac cgcgggagca gcggcaacac aaacctttgt gttgctgcca acttccactg
    13021 tgacccctac ctttacaaga gcgccttcag agactccatc tccgacgaat actttcatct
    13081 ttgtgcttgc aacgccaacc gtaccttcgc tcacacccac tccagagaaa tctgatgaac
    13141 cttatgattg tcaggtgctc tcacaaaatc ctgtggacga tacaagtttt gtacccggtt
    13201 tggcgtttca aacccgctgg gtggttaaaa acatcggaac ggagacctgg ggcgtaaata
    13261 cagcagacta tcgctttgta agcgggacca agttccataa gctgagtgta tatgatatgc
    13321 ccacctccat tccaccgggg gaaaaggtgg aaatctttgt cgaaatgtgg gctcccatgg
    13381 accccggcac ctataccacc acatggcgga tcaaggttgg aaagaccgag ttctgttcca
    13441 tgaaattgaa gattattgtg ccctaaacaa aaaatcctcc gagtgaaaac tcggaggatt
    13501 ttttgttgtc taataattat gaagcggggg gaaggggttc gtccttggcg ggggtttctt
    13561 caacgacagc tactgcttcg ggtttcttgc gcagggtgaa gaagccgact gccaccggga
    13621 taagaatcat gatgaaagaa aggcaaagaa atacagcacc catcgcgggc ggcatgggct
    13681 ggctgttatt ccccaatgag aaagacccgt ttccagaaaa ggtaccaatg ctcattgcgc
    13741 aattgaaaag ggcgcaacag ccacagatca gggcggtggc aatggtggca atcatgccag
    13801 tgttcttgtt gttcatattt ttctcctcgt aagttgatta ttttcacttg cagtatacgc
    13861 tcaacaaaaa cgggatgcaa gcaattgctg catcccgttg gtactagttg atctaacttc
    13921 tcaacttcgc gccgacttct ttttccagcc gtttgacgat cttgttgcgg atggcggcgg
    13981 catcggcgtc ggtcatggtt ttgtcttcgg cttgata
//
LOCUS       DAOQJC010000106        13786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2149_length_13786_cov_8.471779, whole genome shotgun sequence.
ACCESSION   DAOQJC010000106 DAOQJC010000000
VERSION     DAOQJC010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13786
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2149_length_13786_cov_8.471779"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLE39_14440"
     CDS             complement(<1..314)
                     /locus_tag="PLE39_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69580.1"
                     /translation="MKQIEEYYAPWVKGIPMYISEHIELAWRKPELHRMMSNENPLPP
                     SDKVLEAMFKYAKMTNRYPDQGLIVRSKIAEINGVAGPQNVMIGNGSSEVYDNIFRMF
                     IEP"
     gene            complement(402..1277)
                     /locus_tag="PLE39_14445"
     CDS             complement(402..1277)
                     /locus_tag="PLE39_14445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNE69581.1"
                     /translation="MSIVNAKEIMIPAAKEGWAVGAFNVTDLLQFEAVIDAAIEKKAP
                     VIVQTSVKPSQFLGTNMMVAIYRTLAESAPVPVCLHLDHSTSIDYCKKCADAGYTNIM
                     IDASKQSYEENIRQTKEVVDYCHSVGNISVEGELGTVGGVEDQIKVAEDEAQLANPEQ
                     SIEFVERTGVDIFAPAIGTAHGVYKTKNPKIDFERMATIHKMLNSNGIKTPLVVHGGT
                     GLPDDYVAKLLAAGGAKFNVSTELKHTLINAKWEYLNAHRDEYDPGKLDVFVRDATRK
                     AVMHWMDKLGCNGKA"
     gene            complement(1371..2300)
                     /locus_tag="PLE39_14450"
     CDS             complement(1371..2300)
                     /locus_tag="PLE39_14450"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HNE69582.1"
                     /translation="MTLAIDKAIAKVPFLAGSKNIKKTPLSGGITNLNFRIDADGRSY
                     VIRLAGEGTDQLGIKRDVEYAANKAAGELGIAPEVVYFIEPEGYIVTRFSNGKRIMPE
                     DIVKPDYLARVAKKIRLFHRRGPKLKGEFNVFKRVEMLTKVSKANNARFPHDWDFIMQ
                     KMNEAKKALEKDPYVPTPCHNDLLNLNWLDEDVPGDIGEIRLLDWEYAGMGDIFFDLG
                     NFSHHHRLNEDQVDIFLYEYFGEITDKTYARLRLMWPMSELHEAMWGTTQTGISKLEE
                     DFQGYADLWFGRFRQHVTDFRWEQWLKDVAKKK"
     gene            complement(2418..4850)
                     /locus_tag="PLE39_14455"
     CDS             complement(2418..4850)
                     /locus_tag="PLE39_14455"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE69583.1"
                     /translation="MSEFPTQAQVVIIGGGVGGSSIAYHLTKLGWKDVVIIEKHELTS
                     GSTWHSAGLVGQMRSDANLTRMMHYSTDLYRSLKAETGQDTSWREVGGIRLASSAERM
                     EENRRLVGMARSFGVPMELISPKEAQDLFPLMDITGVVGAAYTPNDGSIDPTGLTNAL
                     AIGAKNRGARIFTNTTVTAINLKNGRVSEVVTDKGTIKTEVVVNASGMWGREIGKMVG
                     LSLPVLPMAHLYIMTKPIEGVTSKFPNLRDPDLLVYWREEVGGLITGGYERQPATFGM
                     NGIPKDFKFQLLPPDWDRFTPLMENSIRRVPAVANAEVKLLLNGPEGFTPDGEFLLGP
                     TTVKGFWVACAFCAHGLAGAGGIGKVMAEWIVDGSPEWDMWRLDVRRFGPNYNNLDYV
                     AARTFETYTQYYDIHYPGEERISRRNVRTSPTYFRLRDLGCSFGEKMGWERPNWYRIY
                     EEKATHGHEPKGWMRHNWSRAIGHEHLMTRANAGLFDETSFNKFEVRGSGALKFLNYV
                     CANQIDVPVGNLVYTQALNKRGGIECDFTVTRLAEDRFFIVTGTAFGQHDMSWLSLQM
                     PEDGSVTIEDVGSNYACIGMWGPKARTILEKVTTEDVSNAGFPYMTFKRMNVGDVPVL
                     ASRVTYVGELGWEFYCPMEYGLRLWDTLWEAGQSEGMVAGGYKAIDTLRLEKGYRYWS
                     GEISPDYTPYEAGLGFAVKLDKENFIGKDALVKQKAEGIKRKLCTIILDDDRTIVIGK
                     EPIRAGDKLVSWVASGGFGYSINKSIAYAYLPMEFAKAGTKLSVECFGEQVSAEVAQA
                     VLWDPKGERIRQ"
     gene            complement(4936..5745)
                     /locus_tag="PLE39_14460"
     CDS             complement(4936..5745)
                     /locus_tag="PLE39_14460"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE69584.1"
                     /translation="MAKLVKDLMHPGIITCKPTATLGQVAVLLHQHNVHALFVIDRDG
                     RIMGVISDFDLMAGEWLSSDPDSLNVMRKLTAADLMSNPVDTISANTPLPEAVDQFMK
                     KGVSRFLVTENEKPVGILSLSDFVASIADNAKSKRDTVADVMSDAILVCRGKTPVASA
                     ARAMTSAGWRSVLVVDAKGKILGVVSGHDLLRFVKDGLGENLTVRDVMHPALTIDIHA
                     SLRDAANMMIQNHHHRLVVIDKDDPDAFPLGAISTFDIVAEMANPSSVWQK"
     gene            5889..6677
                     /locus_tag="PLE39_14465"
     CDS             5889..6677
                     /locus_tag="PLE39_14465"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNE69585.1"
                     /translation="MGKPLIPAQRRERIQEYLAIHQIARTADLMNLLETSEATVRRDL
                     EWLEQKGILERTHGGAILNQRVIFEQEYQQRAQHFPEEKKMIGELAASLIEEGDIVFI
                     NSGTTATQVLQHIRRDTRVTIFTNNVNAALELGDAGFHYYLTGGEFQSRSNSLAGRFA
                     LDNLNMVFANKVILGVDGVSIKHGCTVPTNQEAEVVRKMIERTKGQVIVVADHSKWGA
                     VSNFPVANMGEVDKLVTDDGFGKEAIKDLAEHSVEVLLARNGKV"
     gene            6743..9172
                     /locus_tag="PLE39_14470"
     CDS             6743..9172
                     /locus_tag="PLE39_14470"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE69586.1"
                     /translation="MKTQAEIVIIGGGIYGAQAAYHLAKNGRKDVVIIEKGEIASGES
                     SHAAGLVTQFATSQAMLKFRMYSVQMYKELGLFSTVGSLRVASSKEQLLEMERSVSRA
                     KALGLDCEVIGPEETKRAMPQISEKNLFGGIYLPGDGQLDPYTTTTSMAKFARDLGVE
                     IYTNTRVTGIKVSAKGEVQAVVTDKGEIRCEIIINAAGMWAPRIAAMAGLHVPTTPVD
                     HQHIALKAVPGHEFAPDTPCLRDPDNLVYMRQEQGGLVIGGYEPTPLPRWIDGTPWEH
                     GSKSFPGDFDQFEMLLEGAIRRLPFLDQAGIITLVRHPGAYTPDCQPLLGPMAGVKGF
                     WMMAGMSLNGYGGAGGMGKLMAEWIIDGEAPMDVYGYRATRFGNYYSDFKYAAERTVE
                     SVKYYYRLKFPHDEHEHGRPHRTSPVHYRLMENGAVFGEKFGWERVNYFDPGKEWRRM
                     GEDQREWGWAKPPFFERMRQEHIATRERVALFDLTSFGKIEVKGKGALPLLQRLTSSN
                     VDKPIGSAVYTQFLNSRGGIESDLTVTRLGEDYFWVITGSGFIANDLARIQMHVDEND
                     GEVSIRDITMEHACLALWGPKAREVLRKITSSDVSNESHPYLMTKPIAINGVKVLAQR
                     VSYAGELGWELYIPNNRATMVWDMLIEAGKEFGMELGGYKVLDPLRLEKGFKYFTTDI
                     TPTVTPYEAGLGFCVDLNKGDFIGKEALVAQKAQGVKRKLCTLVLTDTEDYVQIYGGE
                     AVHHEGKVITRVRSGGYGFTVKKNILYAFLPTELAVKGNRFVIELIEGNREAEVTSSV
                     LVDPKGERLRA"
     gene            9228..10307
                     /locus_tag="PLE39_14475"
     CDS             9228..10307
                     /locus_tag="PLE39_14475"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNE69587.1"
                     /translation="MPSAKQTPPSYVGFGMLTPVYIMALDQLPKHNTGAIVHQVSEYV
                     YDDAAIIACNLRQWKVPTGMIGTSVGDDLLGHHIENTLKNMGVQGKVRFTKKYKTPLE
                     VNVSDKLGARTYFWQRSPEILSTLDTADLGMLKDADLMYVDWYDGDHIIRAMNEARRC
                     GVPVFLNLEHGHRDPEIMEKYVSRATICQAVTDDAQIGRKGAMLTVAKKLLKAGIKTA
                     IITMARQGCMVVQGDEIIRVYAPKVKAVDGSGAGATFSSGFIYGHLKGWGLEETVRFA
                     IAAASLKVTRSGLKMFPISEIKKLAASLSVERMEYRDNQFFELNDLISQAQKSPLVKE
                     SKKLAEKLMLKKKTERKKIKKSLVE"
     gene            10359..11795
                     /locus_tag="PLE39_14480"
     CDS             10359..11795
                     /locus_tag="PLE39_14480"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNE69588.1"
                     /translation="MSFAAFLTQEQVERIHEASLEILEEVGLKVRYEPARAMFEKHGC
                     SVDGERVKFPREVVEKYRKMCPPTFTFYARDPKFDRTIPRDSPVIVTASSAPDIIDPV
                     TGHERRAESGDIARIAHLINELPGYDMFSISTLADDAPAGQFTISRLYPALKYCLKPI
                     RVTTTDHKDTLSVMKMAYMVAGGEEAYKEHPFLTHHYCPTVSPLSMDHLSTENVMYFA
                     SEGLPVYPTIVPNAGLTSPMSMAGTLVQGNAEFLATTVLMQMVKEGTPTIYATLATVA
                     DMRTGAYTSGAIECGMLHMAFAQMSRFYNVPCGGYVGLTNSKLNDAQSGYETGMSAMG
                     GLLGGMDMFNVGGLIDALKTFDFAKAVIDDEVAQMLKRAKRGVNFSEDELAIGLIKEI
                     GPGGSFITAKHTISRMKTEAVMTKLADRDARTTWEKKGSTDIHARAMKRVKEVMTSNT
                     TPLISPELDDKLRAEFPGMVAGMLHPIE"
     gene            11808..12428
                     /locus_tag="PLE39_14485"
     CDS             11808..12428
                     /locus_tag="PLE39_14485"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNE69589.1"
                     /translation="MFGFFKKKESVFQNLIEQQASLAYEGLRLLVKYFETNDPEVAEE
                     LSIKEKEADEVRRILIDELNRSFITPFDREDIFALSRSIDDVIDYADTTVMEMVVLKV
                     APTPFMLRIASLLKDAAYEIMHGVQRLPKHPKVAIDHAQRAKALENRVEAVYREAIAD
                     LFSGPEDIHHLVEMLKMREVYRHLSNAADRGDEAANIIADIVVKKT"
     gene            12499..13431
                     /locus_tag="PLE39_14490"
     CDS             12499..13431
                     /locus_tag="PLE39_14490"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNE69590.1"
                     /translation="MRDSSNIVATMISSRAFHPQTALGVTAVAEFLGPFLFGVTVAKT
                     VGSDIVDSSLLSLDALVACLAAAIIWNLITWFLGIPSSSSHALIGGLIGVAAFTVGFG
                     SINVHAIYKVLTGLFVSPLAGFVVGFMILRMIYFLAQNASPNINELFKRAQLLTAIGL
                     AFSHGTNDAQKTMGIITLSLIIGGELTGFQVPFWVIAVSASTMALGTAMGGWRLIRTL
                     GGKFYKIRPVHSFATQITSAVVILAASFGGAPVSTTQVVSSAIVGVGSSERFGKVRWG
                     VVSDILTAWIITIPVSGLISAGIYWFVESWIRGI"
     gene            13594..>13786
                     /locus_tag="PLE39_14495"
     CDS             13594..>13786
                     /locus_tag="PLE39_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69591.1"
                     /translation="MRTKNYAYQLFALFVIAATLLAACGGGAPAATEAPAATEAPAMT
                     EAPAATEAPAATEAPADPMA"
BASE COUNT         3244 a         3276 c         3670 g         3596 t
ORIGIN      
        1 ggttcgatga acatgcggaa gatgttgtcg tacacttcgc tggaaccgtt accgatcatt
       61 acgttctgcg gacccgcaac accgttgatc tcggcgatct tgctgcggac aatgagaccc
      121 tgatcggggt agcggttggt catcttggcg tacttgaaca tggcctccaa caccttgtca
      181 gaagggggca gaggattttc gttggacatc atgcggtgca gttcgggctt gcgccatgcc
      241 agttcaatgt gctccgaaat atacatcggg atgcccttta cccacggggc gtaatattct
      301 tcaatttgtt tcattacttt gatctcctga tttattttgt ttatttcagg ctgcaaggag
      361 cttaactgtc aacctgccaa cctgtaactt gcaacctgtt attacgcctt gccgttgcag
      421 ccgagcttgt ccatccagtg catgactgcc ttgcgggtcg cgtcgcggac gaagacatcg
      481 agcttgccgg gatcgtattc atcgcgatgg gcgttcagat attcccattt ggcgttgatg
      541 agggtgtgtt tcaactcggt ggaaacattg aattttgcgc cgccagccgc aagcaacttg
      601 gcaacataat catcgggcag tccggtgccg ccgtggacca cgagcggggt cttgatgcca
      661 ttgctgttca acatcttgtg aatggttgcc atgcgctcga aatcgatctt cggattcttg
      721 gtcttgtaca cgccgtgcgc ggtgccgatg gcaggggcga agatatccac accggtgcgt
      781 tcaacgaatt caatggattg ttcggggttc gcgagctgcg cttcatcttc cgcaactttg
      841 atctggtctt ccacgccgcc cacggttccc aactcacctt ccacagagat attgccgaca
      901 ctgtggcagt aatccaccac ttccttggtc tggcggatat tctcttcgta ggactgcttg
      961 gaggcatcga tcataatgtt ggtgtagcct gcatcggcgc atttcttgca gtagtcaatg
     1021 ctggtggaat ggtcaaggtg cagacagacg ggtacaggcg cagactcggc cagtgtgcgg
     1081 tagatggcca ccatcatgtt ggtgccgagg aattgtgagg gttttacaga ggtttgcaca
     1141 atgactggag ccttcttttc aatggctgca tcgatcaccg cttcaaattg cagcaagtcg
     1201 gtgacgttga atgcacctac agcccaccct tcttttgccg ccgggatcat aatttctttt
     1261 gcatttacga tagacataat tttctcctta caaggtatcc ttttgttcga tttttgaaac
     1321 tttgtgaaaa ggatgaatgt ttttttacct tcatccttta tctttcaatc ttattttttc
     1381 ttggcgacat ctttcaacca ctgttcccag cggaagtcgg ttacatgctg gcggaagcgg
     1441 ccaaaccaca ggtcggcata accctgaaaa tcctcttcca gtttggagat gcccgtttgt
     1501 gtggtgcccc acatcgcttc atgcaattca gacatgggcc acatcaggcg caggcgggcg
     1561 tatgttttat cagtgatctc accaaagtat tcatacagga agatatccac ctggtcttca
     1621 ttcaaacgat ggtgatggct gaagttgccc agatcaaaga aaatatcgcc catgcctgcg
     1681 tattcccagt ccaacaggcg gatctcacca atatcaccgg ggacatcttc atcaagccag
     1741 ttcagattaa gcaggtcatt gtgacagggc gtcggcacat atgggtcctt ctccagtgct
     1801 tttttggctt cattcatttt ttgcatgatg aaatcccaat catgcgggaa tcttgcgttg
     1861 tttgcttttg aaaccttggt caacatttcg acgcgtttga acacattgaa ctcgcccttg
     1921 agctttggtc cgcggcgatg gaataatcgg atcttctttg caacgcgtgc cagataatct
     1981 ggcttgacaa tgtcctcagg catgatgcgt ttgccgttgc taaaccgcgt tacgatatac
     2041 ccttcaggtt cgatgaaata gaccacttca ggggcaatcc caagttcgcc agccgctttg
     2101 tttgcggcgt actccacatc tcgtttaatg ccgagttggt cagtaccttc ccccgcaaga
     2161 cgaatcacgt aggagcgacc atccgcatca atcctgaagt ttaaatttgt gatgccaccg
     2221 ctcagcgggg ttttcttgat atttttcgag cctgctaaaa acggtacttt tgcgatggct
     2281 ttatcgattg ctagagtcat tgtcatgaaa tccttccttt ggttggtttt ggattttttc
     2341 caaggcatcg ggagtctttt ggattcccga tgccttgatg attcaacgca tcgttcctaa
     2401 ctcgtacctg atcctgttta ctggcgaatt ctctcgccct tcgggtccca caacacagcc
     2461 tgcgccacct ccgcgctgac ctgttcaccg aaacattcca cgctcaattt tgtgcctgcc
     2521 tttgcaaact ccatcggtaa atacgcatac gcaatggact tgttgatcga atacccaaat
     2581 ccacccgaag ccacccagct caccagctta tcgccagcgc ggatcggctc cttgccaatg
     2641 acgatcgtgc ggtcatcatc gaggatgatc gtgcagagtt tgcgtttgat cccttcagcc
     2701 ttttgcttga ccaatgcatc cttgccgatg aaattctcct tgtcaagttt cactgcaaag
     2761 ccgaggcccg cttcgtaggg ggtgtaatcg ggcgaaatct cgccgctcca atatcgatag
     2821 cctttctcaa ggcgcagggt gtcaatggcc ttgtatccac ctgccaccat gccttcagac
     2881 tggcctgctt cccagagtgt atcccagagg cgcagaccgt attccatcgg gcagtagaac
     2941 tcccatccca gctcgcctac ataagttacg cgcgaagcga ggacagggac atcccccacg
     3001 ttcattcgtt tgaaggtcat gtacgggaag ccagcatttg aaacatcctc ggtggtgacc
     3061 ttttcaagga tggtacgggc cttcggtccc cacataccga tacaggcata gttggaaccc
     3121 acatcttcaa tcgtcacact gccgtcttct ggcatttgta gtgacagcca ggacatatcg
     3181 tgttgaccaa acgcggtacc tgtcacaatg aagaagcggt cttccgcaag tctggtgacg
     3241 gtgaaatcgc actcaatgcc gccgcgtttg ttgagcgcct gtgtgtacac aaggttcccg
     3301 acaggtacgt cgatttggtt cgcgcaaaca taattgagga atttcagtgc gccagatccg
     3361 cgtacttcga atttattgaa ggaagtctca tcgaacagac ctgcatttgc gcgggtcatc
     3421 aaatgctcgt gaccgatggc gcgtgaccaa ttgtgacgca tccagccttt gggctcgtga
     3481 ccatgggtgg ctttttcttc ataaattcga taccaattcg gacgttccca gcccatcttc
     3541 tcgccaaaag aacagccaag gtcgcgcagg cggaagtaag tgggggaggt tcgcacattt
     3601 cggcgagaga ttctttcctc tcccgggtag tggatatcgt aatactgggt gtacgtttca
     3661 aatgtgcggg ctgctacata atcaagattg ttgtagttcg ggccaaagcg gcgcacgtcg
     3721 aggcgccaca tgtcccattc ggggctgcca tctacgatcc attcggccat gacctttccg
     3781 ataccgcccg ctccagcaag accatgtgca cagaatgcac acgccaccca aaaacccttg
     3841 accgttgttg ggccaagcag gaattcgcca tcgggggtga agccttcggg gccattcaac
     3901 agcaatttca cttctgcgtt tgccaccgca ggcacacggc ggattgaatt ttccatcaac
     3961 ggggtgaagc ggtcccaatc tggcggaagc aattggaatt tgaaatcttt cggaattcca
     4021 ttcatgccga atgtggcagg ttggcgttcg taaccgcctg tgatcaaccc gccaacttct
     4081 tcacgccaat acaccagcag gtcaggatca cgcaggttcg ggaatttact ggtcacacct
     4141 tcgatgggct tggtcatgat gtaaagatgt gccattggaa ggactggcag actcaacccg
     4201 accattttgc cgatttcgcg tccccacatc cccgaggcat tgacgacgac ttcggttttg
     4261 attgtgccct tgtcggtaac gacttcactt acacgcccgt tcttgaggtt gatggcagta
     4321 accgtggtgt tggtgaaaat tcttgcaccg cgattctttg cgccaatggc gagggcattg
     4381 gtcaacccgg ttgggtcaat gctgccatcg ttgggggtgt acgctgcacc gacgacgcct
     4441 gtaatgtcca tcaatggaaa caggtcttgt gcttccttgg gggagatcaa ttccatcggt
     4501 accccgaagg aacgggccat gcccaccagc cgcctgtttt cttccatgcg ttcagcagaa
     4561 gaagcaaggc ggatgccgcc gacttcacgc catgaggtgt cttgtcctgt ttcggcttta
     4621 aggctgcgat acaaatctgt gctgtagtgc atcatgcggg tgaggttggc atctgaacgc
     4681 atttgaccaa cgagtcccgc cgagtgccat gtggagcctg atgtcagttc gtgcttttca
     4741 ataatgacaa catccttcca ccctagcttt gtgaggtggt aggcaatact tgaaccgcct
     4801 acacctccgc cgatgataac gacctgtgcc tgtgttggaa attcggacat gctgtgctcc
     4861 tgtatatgga ataatcaata taaccaaaga cagaggacga agggtaacag catggtcatc
     4921 cttcgtcctc tgttgttatt tctgccaaac cgagctggga tttgccattt ctgccacaat
     4981 gtcgaatgtg gaaatcgcac caagggggaa tgcgtctggg tcgtctttat caattacaac
     5041 caggcggtgg tgatgatttt gaatcatcat gtttgcagca tcacgcaggc tggcgtggat
     5101 atcaatggtc agtgcagggt gcattacatc gcggacggtc aggttctcgc cgaggccatc
     5161 tttgacaaaa cgcaacaggt catgtccgct gacaacgccg aggatcttgc cctttgcatc
     5221 caccaccaga acagaccgcc atccagcgga ggtcatggcg cgggcggcag aagccactgg
     5281 tgttttgccg cggcaaacca aaatggcatc tgacatgaca tcagcaaccg tgtcacgttt
     5341 tgatttggca ttgtcggcaa tgcttgcgac aaaatctgac agggagagta tgcccacagg
     5401 tttttcgttc tcagttacca ggaagcgtga aacgcctttt ttcataaact ggtccactgc
     5461 ttcggggagg ggggtgtttg cagagatggt atccaccggg ttcgacataa gatccgcagc
     5521 ggttagtttt cgcatcacat tcaagctgtc ggggtcggag gaaagccatt cgcctgccat
     5581 caaatcaaaa tctgaaatga cccccataat gcgcccatca cggtcaatga caaaaagggc
     5641 atgcacattg tgttgatgca aaagtacggc cacctgtcct aatgtggctg ttggcttgca
     5701 ggtgatgatg ccggggtgca tgaggtcttt gacaagtttt gccatggcag ctccaataaa
     5761 atcaaaacca agacctgaca ggtttgacgg tgattattga gcctgtcagg tctgagcgga
     5821 attgaacgaa ttataatgaa aaaatcaaaa acttgcaaat tccttcaaat tcttatataa
     5881 tccgccagat gggaaaaccc cttatcccag cccaacgaag agagagaatt caggagtatc
     5941 ttgcaattca tcaaatcgcg cgcactgccg acttaatgaa tttgcttgaa acttctgagg
     6001 caaccgtccg ccgcgatctt gagtggctcg aacaaaaggg gatccttgag cgtacgcatg
     6061 ggggcgcgat cctgaatcaa cgcgtgatct ttgagcagga atatcaacag cgcgcgcagc
     6121 attttccaga agagaaaaaa atgatcggcg aacttgccgc gtctttgatc gaagaaggcg
     6181 atattgtttt tattaacagc ggaacgactg ccacacaggt tcttcaacat atccgccgtg
     6241 atacgcgggt gaccattttt acaaataatg taaatgcagc tttggaattg ggtgatgcgg
     6301 gttttcacta ctatttgaca ggcggtgaat tccagtcacg ctccaactca ctggcaggcc
     6361 gcttcgcgct ggacaatttg aacatggtct ttgccaacaa ggtgattctc ggcgttgacg
     6421 gagtttccat aaagcatggt tgtacggttc ccaccaatca ggaagccgag gttgtgcgga
     6481 agatgatcga gcgcaccaag ggccaggtga tcgtggtggc agaccatagc aagtggggcg
     6541 cggtgtcaaa cttccctgtg gccaacatgg gtgaggttga caaacttgta actgacgatg
     6601 gtttcggcaa ggaagcgatc aaagaccttg ctgaacattc tgtggaagta ttgctcgcca
     6661 ggaatggtaa agtgtaaaga cgaaaactga tgaacaggcg catgatgggc ctgttcattt
     6721 atctactctc aatggagcga atatgaaaac tcaggcagag atcgttatca tcggtggtgg
     6781 aatttatgga gcgcaagccg cctatcatct tgcaaagaat ggccgtaagg atgtggtcat
     6841 cattgagaaa ggcgaaattg ccagcggtga atcttcacat gcggcgggtt tggtgacgca
     6901 atttgcgacc tcgcaggcca tgctcaaatt ccgcatgtac agcgtgcaga tgtataaaga
     6961 gctgggattg ttcagcaccg tagggagttt gcgggtagcc tcgagcaagg aacagttgct
     7021 cgaaatggaa cgcagtgtca gccgcgccaa agcattgggt ttggattgcg aagtcattgg
     7081 ccctgaagaa accaaacgcg ccatgcctca aatctcagaa aagaatttgt tcggcggcat
     7141 ttacctgccc ggtgatgggc agttggaccc atataccacc accacgtcca tggcgaagtt
     7201 tgccagggat ctcggtgtgg aaatttacac caacacccgc gtgaccggca tcaaagtttc
     7261 agcgaaaggg gaggttcagg cggttgtaac agacaaaggt gagattcgat gcgagatcat
     7321 catcaacgcg gcggggatgt gggcgccgcg tattgctgcc atggcggggt tacacgtccc
     7381 aaccacccct gtggatcatc aacacattgc cttgaaagct gtgcccggac atgaatttgc
     7441 gcccgatact ccctgcttgc gtgaccctga taatctggtt tacatgcgtc aggagcaggg
     7501 cggtttggtc attggcggtt acgagccaac cccgcttcca cgttggatcg atgggacacc
     7561 ctgggaacat ggcagcaaga gtttcccagg cgattttgat caatttgaaa tgctgctcga
     7621 gggtgctatt cgtcgacttc cgtttttgga tcaggcgggt attatcacac tggttcgcca
     7681 tcccggcgca tacacccctg attgtcaacc cttacttgga ccgatggctg gtgtcaaagg
     7741 gttctggatg atggcgggga tgtcgctcaa tggttatggt ggcgcaggtg gaatgggtaa
     7801 gttgatggcc gaatggatca ttgacggcga agcgccaatg gatgtgtatg gatatcgtgc
     7861 cacccgcttt ggaaattatt attctgactt taagtatgca gcggaacgca ctgttgaaag
     7921 tgtgaagtat tactatcgtt tgaaattccc gcacgacgag cacgaacatg gtcgcccgca
     7981 tcggaccagc ccagtgcatt atcgtttgat ggaaaatggc gcggtctttg gcgaaaaatt
     8041 cggctgggaa cgtgtaaatt attttgatcc tggcaaggaa tggcgccgaa tgggcgagga
     8101 ccagcgtgaa tgggggtggg caaagcctcc gttctttgaa cgtatgcggc aggaacatat
     8161 tgccacacgt gagcgcgtgg ccttgtttga cttgacatcg ttcggcaaga tcgaagtcaa
     8221 aggtaaaggt gcgctgccgt tgttgcagag actgacttca agcaatgttg ataagcccat
     8281 aggcagtgcg gtatacaccc agttccttaa ttcacgcggt ggcattgagt ctgacctaac
     8341 ggttacccgt ttgggagaag attatttctg ggtcatcacg ggttcgggtt ttattgccaa
     8401 cgaccttgct cgtattcaaa tgcatgtgga tgaaaatgac ggagaggtgt ccatccgtga
     8461 tattacgatg gaacatgcct gccttgcttt gtggggaccg aaggcacgtg aagtgctgcg
     8521 gaaaattaca agcagtgatg tttcgaacga gtctcatccg tacctgatga ccaaacccat
     8581 cgcgatcaac ggtgtgaaag tgttggcgca gcgtgtcagt tatgcgggtg agttgggctg
     8641 ggaactgtat attccaaaca atcgcgccac catggtttgg gatatgctga tcgaggcagg
     8701 gaaggaattt ggtatggaac ttggcggtta taaagtgctc gatccattgc gcctggagaa
     8761 gggtttcaag tactttacca ccgacatcac acccacggtt accccttatg aagcagggct
     8821 tggtttttgt gttgatttga acaaaggtga ttttatcggc aaagaggcat tggttgcgca
     8881 aaaagcacaa ggtgtgaagc gtaaactttg cacactggta ttgaccgaca ccgaggatta
     8941 tgttcagatt tatggtggtg aggcggttca tcatgaaggc aaggtcatca cccgtgtgag
     9001 aagtggcgga tacggcttta ccgtcaagaa gaatattttg tatgctttcc tgccgacaga
     9061 gttggcagtg aaaggcaacc gttttgtcat tgaactcatt gaaggcaacc gcgaggcaga
     9121 agtgacctca tctgtattgg ttgacccgaa aggcgagcgt ttacgtgcct gatattgcgt
     9181 aatcgcaaag agtttgcagg cctttgcgaa attttgtggt gaaaagaatg ccttccgcta
     9241 aacaaactcc tccatcttat gtaggctttg gaatgttgac gcctgtgtac atcatggcac
     9301 tggatcaact acccaagcac aacactggcg cgatcgtaca tcaggtcagc gaatatgtgt
     9361 atgatgatgc tgcgatcatt gcttgcaatt tgcgccaatg gaaagtccca acggggatga
     9421 tcggcacatc tgttggtgat gatttgctgg gtcatcatat tgagaacacc ctgaaaaata
     9481 tgggggtgca gggcaaggtg cgtttcacca aaaaatataa aacacccctt gaagtgaatg
     9541 tttcagataa attgggtgca aggacatact tctggcagcg ttctccagag attctttcta
     9601 ccttggacac tgcggatttg ggaatgttga aagacgcaga tctgatgtat gtagattggt
     9661 atgacggtga ccatatcatc cgcgcaatga atgaagcacg gcgctgtggt gtgcctgtct
     9721 ttcttaatct tgagcatggt catcgtgacc ctgagatcat ggagaagtac gtaagccgcg
     9781 ccaccatttg ccaggcagtg accgatgatg cccagatcgg cagaaaaggc gcaatgttga
     9841 ccgtagccaa aaaactgctc aaagcgggta tcaaaaccgc gatcatcacc atggcaaggc
     9901 aggggtgtat ggttgtgcag ggtgacgaga tcatccgtgt gtatgctccc aaggtcaagg
     9961 cggtggatgg ttcgggggcg ggtgcgacct tttcgtcggg cttcatctat gggcatctca
    10021 aaggatgggg gcttgaagag accgtccgtt ttgcaattgc ggcagcttca ctcaaggtga
    10081 cccgctctgg gttgaagatg tttccgatca gtgagatcaa aaaactggcg gcgtcccttt
    10141 ctgtggagcg gatggaatat cgggataatc agttttttga attgaacgat ctgatctcgc
    10201 aggcacagaa aagtccgctg gttaaggaaa gcaagaagtt ggctgaaaaa ctgatgttga
    10261 aaaagaagac agaacgaaaa aagatcaaga agtcgctggt ggagtgattc accatgaaac
    10321 gaatattgaa acccttatca atttaaagga tgtgaaccat gtcatttgct gcatttctta
    10381 cccaggaaca ggttgaacga attcacgagg cttcacttga gattttggag gaagttggtc
    10441 tgaaggtgcg ttatgaaccc gcacgggcca tgttcgaaaa acatggctgc tcggtggatg
    10501 gcgaacgggt gaaatttccc cgtgaggtgg ttgaaaaata ccgcaagatg tgcccgccaa
    10561 ccttcacttt ctacgcacgt gacccgaagt ttgaccgcac gattccccgg gatagcccgg
    10621 tcattgtcac ggcgagttcc gctccggata tcattgaccc tgttacgggt catgaaagac
    10681 gcgctgaatc tggcgatatc gcccgaatcg cacatctgat caatgaactg ccaggctacg
    10741 atatgttttc catttccaca ctggcagatg acgcaccggc gggtcaattc acaatctcga
    10801 ggttgtatcc tgctctcaaa tattgtctta aaccgatccg tgtcaccacg accgatcaca
    10861 aagatacctt gagcgttatg aagatggcgt atatggtggc gggcggtgag gaggcatata
    10921 aggagcatcc gttcctgacg caccattatt gcccgacggt ttcgccgctt tcaatggatc
    10981 atctttccac tgagaacgta atgtattttg caagtgaagg cctgcctgtt tacccgacca
    11041 ttgtgccgaa tgcggggctt acttcgccca tgtcaatggc tgggacgctg gtgcagggca
    11101 atgcggaatt cctggcgaca actgtgttga tgcagatggt gaaggagggt actccaacca
    11161 tctatgccac tcttgcaaca gtagccgata tgcgcacagg cgcctatact tccggtgcca
    11221 ttgagtgtgg aatgttgcac atggcttttg cgcaaatgtc ccgtttttat aatgtgcctt
    11281 gtggtggtta tgtcggcctg acgaattcca aattgaacga tgctcaatct ggctatgaga
    11341 cgggcatgtc agccatgggc ggccttttgg gcggcatgga tatgttcaat gtcggcgggc
    11401 tgatcgatgc gttgaagaca ttcgattttg caaaagccgt tattgacgac gaagtggcac
    11461 aaatgttgaa gcgtgccaaa cgcggcgtga atttttcgga ggatgaactt gcgatcggct
    11521 tgatcaaaga gatcggccct ggcgggtcgt ttattaccgc gaaacatacc atcagccgta
    11581 tgaagacaga agccgtgatg accaagttgg ccgaccgtga tgcgcgtaca acctgggaaa
    11641 agaaaggttc cactgatatc catgctcgtg caatgaaacg cgtaaaggaa gtcatgacca
    11701 gcaatacaac cccgctgatt tcccccgaat tggatgataa gctccgcgca gaattcccgg
    11761 gaatggtggc aggaatgctc cacccaattg agtagtgaaa ggtatttatg tttggctttt
    11821 tcaagaaaaa agaaagcgtt tttcaaaatt tgatagaaca gcaggcctct ctggcttatg
    11881 aagggttgcg cctgttggtg aaatattttg aaaccaatga tccagaagtg gcagaggagc
    11941 tctcgattaa ggaaaaagaa gcggatgaag tgcgccgcat cctgatcgat gagttgaatc
    12001 gcagttttat cactccgttt gaccgcgaag atatttttgc gctttcccgc tccattgatg
    12061 atgtcattga ttacgccgat acgaccgtaa tggaaatggt tgtgttaaag gtcgccccaa
    12121 ccccttttat gctccggatc gcatctttgt tgaaagatgc tgcttatgaa attatgcatg
    12181 gggttcaacg tttgccaaaa catccaaagg tggcgatcga tcatgctcag cgcgcgaagg
    12241 cgttggagaa tcgtgtagag gcggtttatc gtgaagccat tgctgatctg ttcagcggcc
    12301 ctgaggatat ccaccacctg gtcgaaatgt tgaagatgcg cgaggtttat cgtcatctct
    12361 ccaacgcggc ggaccggggg gatgaggcgg ccaatatcat tgccgatatc gttgtaaaga
    12421 aaacttaaca aggtgttaat gtcttctgaa ttgctccttg ttatcattct ggcgctggtt
    12481 tttgaattca tcaatggtat gcgagattct agcaatattg tagcgactat gatttcctcg
    12541 cgcgcattcc atccgcaaac cgctctgggc gtaacggcag tggcagaatt tttaggcccg
    12601 tttttgtttg gcgtgaccgt ggcaaaaaca gtcggcagtg atatcgttga ttccagcctg
    12661 ttgtcgttgg atgcactggt tgcctgtttg gcggctgcca ttatttggaa cctgattaca
    12721 tggtttcttg gaattcccag cagttcatcg cacgctttga tcggcggttt gatcggcgtg
    12781 gctgccttta cggtaggctt tggttcgatc aatgtccatg cgatttataa ggttttgacc
    12841 gggttgttcg tttcgccgtt ggctggtttt gttgtcggtt ttatgatatt aaggatgatc
    12901 tattttcttg cgcaaaatgc atccccaaat atcaatgagt tgtttaagcg tgctcagttg
    12961 ctcactgcca tcggtctggc tttcagtcat ggaacgaatg atgcccaaaa gacgatgggt
    13021 attatcacgc tgagtctgat catcggcggt gaattgaccg ggtttcaagt cccgttttgg
    13081 gttattgctg tcagtgcatc caccatggcg ttgggtactg ccatgggcgg gtggaggctg
    13141 atccgcacgc taggcggaaa gttttataag atccgcccgg tacatagttt tgccactcag
    13201 atcacttcag cggtcgtaat tttggctgca tcatttggtg gcgccccggt cagcaccacc
    13261 caggttgtaa gttctgccat tgttggtgtt ggttcatcgg agcggtttgg aaaggtacgt
    13321 tggggtgtgg ttagtgatat ccttactgcg tggatcatta cgatccctgt cagtgggttg
    13381 atttcagcag gtatttattg gtttgttgag agttggatta gaggtattta gccaccattc
    13441 ttgttcattt attcaaaggg ccgtttgact ttgaatattg ataatgctaa aatgttaacg
    13501 ctgtgttaac ggatcggagg tgtctgggaa gagaagtctg tcatgtgttt ccattcgtct
    13561 gatcaaccaa aacccaaaag gagatagaga gaaatgcgta cgaaaaatta tgcttatcaa
    13621 ctgttcgcct tgttcgtaat tgcggccacc ttgttggctg cttgtggtgg tggtgccccc
    13681 gccgccactg aagcccctgc tgccactgag gcccctgcca tgaccgaagc ccctgccgcc
    13741 accgaggctc ctgctgctac cgaagctccc gccgacccca tggctg
//
LOCUS       DAOQJC010000107        13716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2164_length_13716_cov_8.221287, whole genome shotgun sequence.
ACCESSION   DAOQJC010000107 DAOQJC010000000
VERSION     DAOQJC010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13716
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2164_length_13716_cov_8.221287"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PLE39_14500"
     CDS             complement(<1..641)
                     /locus_tag="PLE39_14500"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNE69592.1"
                     /translation="MSKKLTGNQIRQDFIDFFVEHGHTAVPSMSLVPGGDATLLFTNS
                     GMVQFKDVFIGTDTKPYTRAVDSQKCMRVAGKHNDLEDVGRDDTHHTFFEMLGNWSFG
                     DYYKKDAIAWSWQLLTEVWGLPKDKLYATCFRDDKGNVPQDDEAADIWKQQPGFDPEH
                     VLFFGRKENFWQMAETGPCGPCSEIHIDLGEERDNMRGTDHVCGVNGECTRYL"
     gene            974..1429
                     /locus_tag="PLE39_14505"
     CDS             974..1429
                     /locus_tag="PLE39_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69593.1"
                     /translation="MTYLPGILMRGHQVASRPSKDYPYSSLEKQKPYFKALGLDLAPY
                     FNGTLNISIAPLEFEMTRPEFTFPLVEWTDLHPPETFSFSRCRVRFEGKEYAGWVYYP
                     HPETKKTHFQNPSLVEVITYEIAGIQYGDALEIEVNPQEITITGYTPAP"
     gene            1426..2124
                     /locus_tag="PLE39_14510"
     CDS             1426..2124
                     /locus_tag="PLE39_14510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNE69594.1"
                     /translation="MTTQKTNLDPLISSPDSNEYFNSISHLIGAILSISALTVLVSLA
                     AIADKPIHVVGFAIYGVSLFLSFLFSCLLHFFLLFGKYLRVFGILDHNAIYILIAGTY
                     TPFCLTIFSGATGWVLFGVIWSLAVFFITLKSIFFTKISVLMSNISFLLMGWIIVFLI
                     GPIYTQLGMGAISLLITGGLFYTVGALIFAYGKPNPFPPYFGNHEIWHLCVLAGNATF
                     FFVMLFYVLPFPTS"
     gene            2311..2700
                     /locus_tag="PLE39_14515"
     CDS             2311..2700
                     /locus_tag="PLE39_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69595.1"
                     /translation="MTFTYVDPREREDIRAHQGEDVWVLLNHVKADKCGEYEHFLHEI
                     LKPAIAHIHPETYNKVRVLHPSLPNKDGTYTYIFLMDPVVADGIYDIGEILHSFYRPE
                     LADQYMKIWNNSLASPQIEHDMIQSSW"
     gene            complement(2830..3102)
                     /locus_tag="PLE39_14520"
     CDS             complement(2830..3102)
                     /locus_tag="PLE39_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69596.1"
                     /translation="MEIAVGRVTHYFDHLKVAVLALNGDLKLGDKIHIVGHGTDLFQR
                     VSSMQIEHRSVVWVKPGDHVALKVIEPVHEHDAVFRIIEEVLEPLV"
     gene            3300..4055
                     /locus_tag="PLE39_14525"
     CDS             3300..4055
                     /locus_tag="PLE39_14525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNE69597.1"
                     /translation="MYTTDMYEGVLAETVTMTGANGDKINAYLAKPLGKGPFPAMVLV
                     HHMPGWDQWYREATFKFAHHGYVTLSPNLYFRSGHGTPEDVAAKVRADGGVPDEQVIG
                     DLTGAMNHLRGLAEVNGKVGIFGTCSGGRHAYLAACRTTGFDAIVDCWGGRVVMTPDQ
                     LNANFPVSPLDYTKDLSCPILGLFGEDDSSPTPEQVKIHEAELKKYGKGYEFHMYPNA
                     GHGFFYYDRPNYRQEQAVDGWKKTFAFLEKHLA"
     gene            4211..4480
                     /locus_tag="PLE39_14530"
     CDS             4211..4480
                     /locus_tag="PLE39_14530"
                     /inference="COORDINATES: protein motif:HMM:NF039404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6295 family protein"
                     /protein_id="HNE69598.1"
                     /translation="MCTMIVEKVRIEGSGKGTQGWFPLQQANVSYDHPFDAPYEHALN
                     IDFVNEAQGPSARVAVELSESAARELVRTILAVLDQAESGGHLVK"
     gene            4952..6214
                     /locus_tag="PLE39_14535"
     CDS             4952..6214
                     /locus_tag="PLE39_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69599.1"
                     /translation="MILALLTGGLVFFIYKSYGSLKILPKTVSDIRARAIAQLVAGNW
                     TAKPDPNIDRKIENEFKLKEIGDGIIGGKKIPIIVITNSLHPFPGYGWLQAQNVFVCK
                     PKEQTTPSGSIHEIIEKVQENIHGKIAEFPLKEVHCGNLIAIHGDSLFMDSAWLNKDK
                     SPHLWADMHTLKNLNSIDSRASARCYFVIQSFFPEHDTVANFFVRPFMAGNSISCQIA
                     LTTIGPLIHRNDYFQERLLQHRREQEDAPISATALPTPLLEFQNSAQKTNLLKQRIKQ
                     SSQKFRSNKMHYAEIEKLNPIETEDEQKNFSKELKELMEVTTTWPGRLTPLENWRESY
                     SLTFTNDFFGSTEALASIKTLYQQISRTILDTFDSMGFDITDYRDREGKYSINAEKID
                     RLIIGQEIKILQTADARLPAKPQENSIS"
     gene            6224..6802
                     /locus_tag="PLE39_14540"
     CDS             6224..6802
                     /locus_tag="PLE39_14540"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE69600.1"
                     /translation="MKVNINAKSINQVVNAETVNIDQRSGESESPPSDESASGGIFIS
                     YRRKDSADVVGRIYDQLVKHFGAQYVFKDVYSIPLGSDFGKYIIEAIGRSGALLAVIG
                     EKWLETNDAGKTRLDEPNDFVRIEIESALVNEIPIIPLLVGGATMPPKEKLPPSLEKL
                     SEKNGIQIRPDPDFHMDMKRLITSLEKHINTH"
     gene            complement(6810..7439)
                     /locus_tag="PLE39_14545"
     CDS             complement(6810..7439)
                     /locus_tag="PLE39_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE69601.1"
                     /translation="MMIRLLIADDHPVVRSGLRAILASQPDFELLAEADNGFSAVESA
                     AKFQPDVILMDLQMPAMDGVSAIAKIHAANPSIYILVLTTYDTDVDILRALDAGATGY
                     LLKDAPPDELFRAIRSAARGEVTLAPSIAAKLTRRLTHPLERSLSGREIDVLSLAARG
                     DSNKEIAIKLHITEATVKSHFVHIFNKLNVTDRTAAVTMALEKKMIRLE"
     gene            complement(7436..8617)
                     /locus_tag="PLE39_14550"
     CDS             complement(7436..8617)
                     /locus_tag="PLE39_14550"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNE69602.1"
                     /translation="MTKKSEIKKPHVWDRWDWIFHTISYGVLIFQAAIFLSNEVKHAS
                     LQIFFGLTAAVMVWYFPLVFKRQALWMQIPARTLAYFVIGWFLWGTLILAHPAAMMLA
                     AMYYPILFMRMPIRWAAASASLLTFGLLAASIFVLGLDENLLPTLVTITGLILGGAIV
                     IGVFINSLISQSVDRQRLIEELSQTRASLLKMEREAGVLAERQRLAREIHDTLAQNFT
                     SIIMHLTAAKLGDSESAVPHVQQAEQTARDGLDEARRIVWAMRPEQLEHSTLVESIEV
                     LAARWSAENSVRVNTAITGTPRPLGPERDAALLRVTQEALNNVKKHANASRVDITLSY
                     MADLTALDIVDDGIGFEEKDQHGFGLKTMRERVEELRGTLTIESSDGTAIAVSLPVED
                     E"
     gene            complement(8658..>9178)
                     /locus_tag="PLE39_14555"
     CDS             complement(8658..>9178)
                     /locus_tag="PLE39_14555"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE69603.1"
                     /translation="SPSVTSDKKALLKELTKAIRDHNAEEVKRLLALGMDVNEIQSFR
                     QLTPLALASYEGDTEIIALLLEHGADIAMYNKNQYGTTALIEAAQNGKTDAVKFLLEH
                     GADINQRDKFGDPALNWATYYGRVDVVKLLIENGADLTVVGSGGGTALNTAIAQGHED
                     IEKMLRDAGATR"
     assembly_gap    9179..9278
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9279..9521
                     /locus_tag="PLE39_14560"
     CDS             <9279..9521
                     /locus_tag="PLE39_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69604.1"
                     /translation="ALVNSFRSAFLSEVTEGDAVGTTCVAVGAINVVVGTAIGVAATH
                     ADDTAKAKRDAKRPKFRLMDFVFRKGSIECLQSVRV"
     gene            complement(9527..9694)
                     /locus_tag="PLE39_14565"
     CDS             complement(9527..9694)
                     /locus_tag="PLE39_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69605.1"
                     /translation="MYRTTVAMYLAAFGIIFFFAMPFKVLEDNVATFFGIAFLILSAV
                     TWGFWPTPKKQ"
     gene            complement(9750..10568)
                     /locus_tag="PLE39_14570"
     CDS             complement(9750..10568)
                     /locus_tag="PLE39_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69606.1"
                     /translation="MSKTKVDILTLIAVSAFSFMLATAIHEHVGHSLACISVNGRLSE
                     MGAFYADCAADSVSDLGGRFVALAGPLVSLAAGMFFLRLFGKMFSKAGAVLKFFLWHQ
                     ATVNLMIAAGYLLFSGVAGIGDLGTVSGGVFYQAEPEWLYRAALTVLGLAGYYAVIRI
                     SIQKMDAFIGGEGVERVNRAQMTSLIAYFSGGAVALLIGLLNPHGIFIVLAASVASSM
                     GGTSGMAWMMQLLDRKKDTGDAPFTLQRDWGWILGSGAFLLIYAAVFGPTIFLK"
     gene            10721..11311
                     /locus_tag="PLE39_14575"
     CDS             10721..11311
                     /locus_tag="PLE39_14575"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNE69607.1"
                     /translation="MELKDTVLKRRMVRNFADTPVSPEIIERIVDLTRHAPSAGFTQG
                     QSFIIVTQPDMRKAIADTCEEGEYVKSGFDPFISKAPVLLIPCTSELAYHRRYQEADK
                     VDDKGNEIVWPVPYWFMDIGCAVMIALLATIDEGLVSAFVGSKDLKTLRTLLSIPEEV
                     TPVGVIAVGYRAPDRPSPSLKRGRKPDQDYIHREKW"
     gene            11322..12554
                     /locus_tag="PLE39_14580"
     CDS             11322..12554
                     /locus_tag="PLE39_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69608.1"
                     /translation="MRFAELFFFGFTLWIGSYLLARQSQKITVQLTGWGLLAYALALA
                     IQILFDQSILVILLIPAMLWIGAALHLLPEENPLRQIFIRTWAVTSIPVVILTLANAW
                     FAAVIVLALVICAGMVAQMAARSQFKNTFALLGVIGLFVTLSVGLLILPLNWIPTSWG
                     MVLLGFDLVLLGITITAWDAFDEGESIRAHLLRSFVSNLYYSGALVILASLFNGSDIL
                     LIALIAFGILTQSFSNALQSVLDRLTLPRQTTDERQTLRQTADALPLLSALKTDSVTE
                     EQFTRLTRRALSHLGDLPKLATNPLTNLPQIKESNPLERAHALKSLLVQSIQKLKPQS
                     EAKFGTTDEWRYFNAVYFPYVLGLKPYTRRADLDSLDEESRAALDWFQTSVPERTLHN
                     WQNTAAKLISDDIKAGRV"
     gene            complement(12572..>13716)
                     /locus_tag="PLE39_14585"
     CDS             complement(12572..>13716)
                     /locus_tag="PLE39_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69609.1"
                     /translation="LPWSIEVDLGNSAEFTLYATNASEFGTVHCEVLKGEFLVGDASG
                     SVFAGCSGSVTGSGDNTKVNFKGFDDVPPDSRSLPTSGGLVAGNELIMAVGGNVLDGL
                     DLYVLDPASPGRMAKLTKGLYSAFCPAISPDGTKIAFRKRDIRWDIFVMTVSDSTLRR
                     ITDNEAEEGCPVWSPDGQNLAFDSRNESAFSEIILSSSDGRTQNLLTAALRAEQPEAN
                     FYSPNWSPDGKQIAFTLRLKDNDIYLMNIEDGSASPLEVTPDQNEAFPRWSPDGESVL
                     FFRKDGIYIGKADGSGSVKVNTGSLIPDNANWSPDGARIVFSADDKETEIKTKALYLV
                     NLDGSGLTLFAEIPNTDLFDPFWQSFASDSPMLKLQSLPVVPTTQQ"
BASE COUNT         3542 a         3657 c         3228 g         3189 t
ORIGIN      
        1 tcgagatagc gggtgcattc gccattcaca ccgcagacgt ggtcggtgcc gcgcatattg
       61 tcgcgttctt cgccgaggtc aatgtggatt tcggagcacg ggccgcaggg acccgtttca
      121 gccatctgcc agaagttttc tttgcgtcca aagaagagga cgtgttcagg gtcgaaacca
      181 ggctgctgct tccaaatatc ggcggcttca tcatcctgcg gaacgttgcc cttgtcgtct
      241 ctgaaacagg tggcgtagag tttatctttc ggcaggcccc acacttcggt gagcagctgc
      301 cacgaccaag caatggcatc ctttttgtaa taatcgccaa aagaccagtt gccgagcatt
      361 tcaaaaaagg tgtggtgcgt gtcatcgcgg cctacatctt caaggtcgtt atgcttgcct
      421 gccacacgca tacatttctg cgaatcaacg gcgcgggtgt agggttttgt gtcggtgccg
      481 atgaagacat ctttgaactg caccatgccc gagtttgtga agagaagcgt ggcatccccg
      541 cctgggacga gcgacatcga gggtacggcg gtatgtccgt gctcgacgaa gaagtcaata
      601 aaatcctgac gaatctggtt gcctgttagt tttttgctca tgagtggctc cgaaagtttg
      661 aaatggctgg cgaattataa atgaggaatg tccaaggttg aaggttgacg gtgtaaataa
      721 aaagtcccga gaacctgttg gtttcgggac tggatttctt catccatctc aagccgtggc
      781 aggttcctag gtcgcgcatg tagcggcggc ggctcgagtt gctgcggctt gcttgagggt
      841 ttgtttcatg gctaggatta tacacgggcg gtattgagtt gacaagaaga gattcaaaac
      901 tggcaaagaa aattcatata aatttgacaa catggtaatc actttacaca cagaacgcgc
      961 tatactcgcg gccatgacct atcttcccgg catcctcatg cgtggacatc aagtggcttc
     1021 ccgcccgtcc aaagattatc cgtacagttc gttggaaaag caaaagccgt acttcaaagc
     1081 gctcggcctt gaccttgctc cctacttcaa cggcacattg aatatttcca tcgcgccgct
     1141 ggaatttgaa atgacacggc ccgagttcac ctttcccctt gttgagtgga ccgacctgca
     1201 cccgcccgag acgttttcat tctcgcgctg cagggtcagg tttgagggaa aagaatatgc
     1261 gggctgggtg tattatccgc accctgaaac caaaaagacc cattttcaaa acccatcgct
     1321 ggtcgaggtc atcacctacg agatcgcagg cattcaatat ggtgatgcgc ttgagatcga
     1381 agtcaacccg caggaaatta ccattacggg atatactccc gcaccatgac cacacaaaaa
     1441 acgaatcttg atccgctcat ctcctcacca gacagcaacg agtatttcaa ttccatcagc
     1501 catctcatcg gcgccattct gtccatttcc gcgctgaccg tgctcgtttc actggcagcc
     1561 atcgcggaca aacccatcca cgtggtgggg tttgcgatct acggtgtcag cctgtttctc
     1621 tcattccttt tcagctgcct cctccatttt tttctgcttt tcggaaaata cctgcgggtc
     1681 ttcggcattc tcgaccataa cgccatttac atcctgatcg caggcacata caccccattc
     1741 tgcctcacca ttttcagcgg cgccacaggc tgggtgcttt ttggggtcat ctggagcctt
     1801 gcggttttct tcatcacgct caaatccatc ttcttcacaa agatctccgt attgatgtcg
     1861 aacatcagct tcctgctcat gggttggatc atcgtcttcc tcatcggccc catctacacc
     1921 caactcggca tgggagccat ttccctgctc atcacgggcg ggcttttcta cacggttggc
     1981 gcgctgatct tcgcctacgg aaagccgaat ccatttccgc cctattttgg caaccatgag
     2041 atctggcact tgtgcgtact ggcaggtaac gcaaccttct tctttgttat gctcttttat
     2101 gttctgcctt tccccacaag ttaaatgaac cttttgcata agctgagacg ggagaagcaa
     2161 aagaaagatt gaatccacct ccctggcgtg ctatagtatc tttaacccaa gttgccgcct
     2221 gcacaaaagc aggaaaaaca aaaacacagc ctcgaatttc ggaaatgatc gcgaatgcaa
     2281 ttttgcgtta tgtataaaaa ggaggcagcc atgactttca cctatgtcga cccaagggaa
     2341 cgcgaagaca tccgtgccca tcagggagag gatgtttggg tgttgctaaa ccatgtcaaa
     2401 gccgataaat gcggggagta cgaacatttt ctgcatgaga tcctaaaacc tgccattgcc
     2461 cacatccacc ccgaaacata caacaaggtt cgtgttttac atcccagcct gccaaacaag
     2521 gatggcacct acacctacat ctttctcatg gaccctgtcg ttgcagatgg aatctatgac
     2581 atcggagaga ttctgcattc attctacaga cccgagctgg ccgatcaata catgaagatc
     2641 tggaacaact cccttgcctc tccacagatc gaacatgaca tgatccaatc atcgtggtag
     2701 catcataatt tacacaaagc ccgatcattt gcaaaacagt atttgcattt taaattcaca
     2761 gcccaacaag catgcgaaaa cagagcggaa ccatttcacg gaaatccgct ctgttttttt
     2821 gtttcaaatt caaacaagcg gctccaaaac ttcttcgatg atcctgaaaa cggcatcatg
     2881 ttcatggaca ggttcaatga ccttaagtgc cacatgatct ccaggtttta cccaaaccac
     2941 tgaacgatgc tcaatctgca tggatgaaac ccgctggaac agatctgtgc catgcccaac
     3001 aatatggatc ttatctccaa gcttcaggtc gccattgagt gccaaaaccg caactttgag
     3061 atgatcgaag taatgagtta ctcttccaac tgcgatttcc ataactcgac tcctttccaa
     3121 agaacacctt ttgcaacttc actgtagctc gatttgtgac ccatttcaag tcggatacaa
     3181 ccccgcctcc ctcttaactg aaacctgcca gcccaaaacc aacattccca gcgccgacag
     3241 ttaaaggcac agcgtagtac aatcgcgcca atttaccaat ttactcagga gagtgaccca
     3301 tgtacacaac cgacatgtac gaaggcgtgc tggctgaaac cgtgaccatg acaggtgcaa
     3361 acggagacaa gatcaatgcc tatctggcaa aaccgcttgg caaaggtccc ttccctgcca
     3421 tggtattggt ccaccacatg ccaggctggg accagtggta ccgcgaagcc accttcaaat
     3481 ttgcccatca cggctatgtg accctctcgc cgaatttgta tttccgctcg gggcacggaa
     3541 ctcccgaaga tgtcgcggca aaagtccgcg cagatggagg cgttcccgat gaacaggtca
     3601 tcggcgatct cacaggcgca atgaatcacc tccgcgggct ggcagaagtc aacggaaagg
     3661 ttggcatctt tggcacctgc tccggcggac gtcacgccta tctcgccgcc tgccgcacaa
     3721 caggctttga tgccatcgta gattgctggg gcgggcgcgt ggtgatgacg cccgatcagc
     3781 tcaacgcaaa cttccccgtt tccccgctcg attacaccaa agacctatcc tgcccgattc
     3841 ttggtttgtt cggtgaagat gattctagcc ccacccccga acaggtaaag attcacgaag
     3901 ccgagttgaa aaagtacggc aagggttatg aattccacat gtacccaaat gcaggtcatg
     3961 gcttctttta ttacgaccgt cccaactatc gccaggaaca agccgtagac ggttggaaaa
     4021 agactttcgc cttcctggaa aaacatctgg cataaaagat gttttgataa gccattttga
     4081 accacgaaaa cactgaagca gtgcggagcc tcctgcggac ggagttttta attattttct
     4141 ctgtgtctcc gtggttaatc tttcagactt ttgcaagaga tctaccttaa gaataaaaag
     4201 gaatcaaacc atgtgtacaa tgatcgttga aaaagtaaga attgaaggca gcggaaaagg
     4261 cacccaaggc tggtttcccc tgcaacaggc caatgtctcc tacgaccatc ccttcgacgc
     4321 cccctacgaa cacgccctga acattgactt cgtcaacgaa gcccagggcc cctcagcccg
     4381 tgtcgccgtg gaattgagcg aatccgccgc acgtgaattg gtccgcacca ttttggctgt
     4441 gctcgatcaa gccgaaagcg gcggtcacct ggttaaataa tcaatataaa ctccctgaag
     4501 aagggacaat catccgcctg tccacgggcc taacttgctt ttccaatttt tttcctgtat
     4561 agtgaaaata aagaaatgga agtgcaaaat gagcgcaacg atacccgaaa cgctgattgc
     4621 ggaccatacg gcgcctgatt gtcccaacca attacgccag aatgtcacaa agcatctgac
     4681 ttatcacatc gcagcttcca tcatctctaa atgtgacttc ccgttaatcg aatcgctccc
     4741 aaaaccgttt gcgccaccct tcaatagcct gggccattat gtattatttg actacaagct
     4801 tttggtaaag atcatctggc aggtgatcgg ggaaataaag tcatcatcat ataaagttta
     4861 caaaagtatt ttgcagaaaa gcctggggtt cggagcactc actgccgtct ctgcctttct
     4921 cctccttggt ccgacatccc aacctgaaat cttgatctta gccctcctca caggcggcct
     4981 ggtatttttc atatataaat cgtatgggag cttaaagata ctcccgaaaa ctgtatcaga
     5041 catccgcgca agagcaattg cccagttggt tgcaggaaac tggactgcaa aaccagaccc
     5101 gaatattgac cgcaagatcg agaatgagtt caaattaaaa gagattgggg atggaatcat
     5161 aggtgggaag aaaatcccaa tcatagtcat tacaaacagc ctgcatcctt ttccaggata
     5221 tgggtggctt caggcgcaga atgtgttcgt ctgcaaacca aaggaacaaa ccacacccag
     5281 cggatcgatc catgagatca tcgaaaaagt acaggaaaac attcatggaa agatcgcaga
     5341 attcccgcta aaagaagtgc attgcggcaa tctgatcgcc attcatggag acagcctgtt
     5401 catggatagc gcatggttaa acaaggacaa gtccccacac ctctgggcag acatgcacac
     5461 cctgaaaaac ctgaactcaa tcgacagcag agcttcggca cgctgttatt ttgtcattca
     5521 atcgtttttc cctgaacacg atacagtggc aaacttcttt gtacggccat ttatggcggg
     5581 taattcaata tcctgtcaga tcgcattaac gacaatcggg ccgctgatac acagaaacga
     5641 ctattttcag gaaaggctgc tccagcatcg cagggagcag gaagatgcac ccatcagcgc
     5701 cacggcactc cccacaccgt tgttggagtt tcaaaattca gcgcaaaaaa caaacttgct
     5761 caaacaacgc atcaagcaat caagtcaaaa attccgatca aacaaaatgc attatgcgga
     5821 gatcgaaaag ctcaatccga tcgaaacaga agacgaacag aaaaatttct caaaggaact
     5881 taaggagctg atggaggtaa ccacaacatg gccaggaaga ttaactccgc tcgaaaattg
     5941 gcgggaaagt tattctctga ccttcacgaa cgatttcttc ggcagcacag aggcgctcgc
     6001 ttcgatcaaa acgctctacc aacagatcag cagaaccatc ctcgacacat tcgattcgat
     6061 gggatttgac attaccgact atcgtgaccg cgaaggaaaa tactcgatca acgccgaaaa
     6121 aatcgaccgc ctgatcatcg ggcaggaaat aaagatttta caaacagccg acgcccgcct
     6181 cccagccaag cctcaggaga acagcatctc ctaaaaggaa gccatgaaag tcaatataaa
     6241 tgcaaaatcc atcaatcagg tggtcaacgc cgagaccgtc aacattgacc aacggagcgg
     6301 ggaatcagaa tccccgccat cagatgaatc tgcatcgggc ggtattttca tctcctatcg
     6361 aagaaaagac agcgccgatg tcgtagggcg aatttatgac caacttgtaa aacattttgg
     6421 ggcgcagtac gtttttaaag acgtgtattc aattccgctg ggatctgatt ttggaaaata
     6481 tattatcgag gcaatcggca gatctggggc actacttgcc gtgatcgggg aaaaatggct
     6541 ggagacaaac gatgcaggaa aaacacggct cgacgaaccc aatgattttg tgcgaatcga
     6601 aatcgaatca gcgttggtca atgaaatccc catcattcca ttgctggtag gtggggcaac
     6661 catgccgcca aaagaaaaac tgccgccaag tctcgaaaaa ttaagcgaaa aaaacgggat
     6721 ccagattcgg cctgaccccg attttcacat ggatatgaag cgtttgataa cgagtctgga
     6781 aaaacacatc aacacacatt gaaaatattt tattccagcc gaatcatctt tttctccaac
     6841 gccattgtca cggcagcggt tctgtcggtg acgttgagtt tattgaagat atgcacgaaa
     6901 tgggatttga ccgtcgcttc ggtgatatgc agtttgatgg cgatctcttt gtttgagtct
     6961 ccgcgtgcgg cgagggaaag cacatcgatc tcacgtccgc ttaaagatct ttcgagcggg
     7021 tgggtcaggc ggcgggtcaa ttttgcggcg atgcttgggg ctagggtcac ttcgccgcgt
     7081 gccgcagagc ggatcgcccg aaacaattca tcaggcggcg catctttcaa aagatagcct
     7141 gttgcacctg catccagcgc gcgcaaaata tccacatcgg tgtcgtaggt ggtgagcacc
     7201 aaaatataaa tggacggatt cgcagcgtgg attttcgcga tcgcagacac accgtccatc
     7261 gcgggcattt gcaggtccat cagaatcaca tcgggctgga attttgcagc agattcaaca
     7321 gcggagaatc cattgtctgc ttcggcgagc agttcaaagt caggttgtga agcgaggatg
     7381 gctctcaatc cgctgcgcac gacggggtgg tcatctgcaa tgagcaatcg aatcatcatt
     7441 catcctccac aggcaaagat acggcaatgg ctgtgccatc gctgctttcg atcgtcaatg
     7501 taccacgcaa ctcttccacg cgttcgcgca tggtcttcaa accaaatcca tgctggtctt
     7561 tctcttcgaa accgattcca tcatccacaa tatccagggc ggtcagatct gccatatacg
     7621 aaagtgtgat gtctacgcgg ctggcattgg catgtttttt gacattgttc aacgcctctt
     7681 gtgtcactcg caaaagggca gcgtctcttt ccggaccgag gggcctgggt gtgccagtaa
     7741 tggcagtgtt cacgcggaca gagttttccg ccgaccagcg cgcggccaac acttcaatgg
     7801 attcaaccag cgtggaatgt tcaagttgtt cggggcgcat ggcccacaca atgcgccgcg
     7861 cttcatcgag gccatcgcgc gcggtctgtt ccgcttgttg aacgtgaggc acagccgatt
     7921 ctgaatcgcc aagtttcgcg gcagtgagat gcatgatgat gctggtgaag ttctgcgcga
     7981 gcgtatcatg aatttcccgc gccaaacgct gacgctccgc caacaccccc gcttcccttt
     8041 ccattttcaa cagactggca cgggtttgag acagttcctc gatcaaacgc tgacggtcca
     8101 cgctttggct gatgagtgag ttgataaaaa ccccgatcac aattgcgccg cccagaatca
     8161 acccagtgat ggtgacaagg gttggcaaca ggttttcatc gaggccgagc acgaagatcg
     8221 aagctgcaag caggccaaag gttaagagcg aagcgctggc ggccgcccag cgaatcggca
     8281 tccgcatgaa caggatcggg taatacatcg cggccagcat catcgccgct ggatgggcaa
     8341 ggataagggt tccccaaaga aaccagccga tgacgaaata tgccagcgta cgtgctggga
     8401 tctgcatcca caatgcttga cgtttgaaga caagcggaaa ataccagacc atgacggcag
     8461 cagtcaggcc aaaaaagatt tgaagtgagg catgtttcac ttcattgctg aggaaaatgg
     8521 ccgcctgaaa aatcagaacg ccgtaggaaa tggtgtgaaa tatccaatcc cagcgatccc
     8581 atacgtgcgg ttttttgatc tccgatttct tcgtcatcat caaaagtata ctcgttcaac
     8641 aaactgcgga cggaggatta tctggttgcg ccagcatcac gcagcatttt ttcaatatct
     8701 tcatgtccct gggcgatggc ggtattcaac gcggtgcccc cgcccgaccc aaccacggtc
     8761 aagtctgcac cattttcgat cagcaacttg accacatcca cacgcccgta ataggttgcc
     8821 caattcaagg ctgggtcacc gaatttatcg cgctgattaa tgtcggcgcc gtgttccagc
     8881 aggaatttta cagcatcggt tttgccgttt tgcgcggctt cgatcaatgc agtggtgccg
     8941 tattggtttt tgttgtacat ggcaatatcg gcgccatgct ccaacaacaa agcgatgatc
     9001 tctgtatcgc cttcgtaaga tgccagagcc aatggtgtaa gctgcctgaa agactggatc
     9061 tcgttcacat ccatccccaa cgcaagcaga cgtttcacct cctctgcatt atggtcgcgg
     9121 atggctttgg ttaattcctt cagaagtgcc tttttatcag aagtcaccga aggcgatgnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc tttggttaat tccttcagaa
     9301 gtgccttttt atcagaagtc accgaaggcg atgcggtggg cacaacttgc gttgcagtgg
     9361 gcgcaatcaa cgtggtggtg gggacagcaa tcggtgttgc ggctacacat gccgatgata
     9421 cggctaaagc gaaaagggat gccaaaaggc ccaaatttcg tttgatggat tttgttttca
     9481 ggaaaggcag catagagtgt ctccaatcgg tgcgggtgtg aaatggttat tgtttcttcg
     9541 gcgtgggcca aaatccccat gtaacagcag agagaattag aaaggcgatt ccgaaaaaag
     9601 tcgcgacatt gtcttcaaga actttgaagg gcatggcaaa gaaaaaaatg atcccaaagg
     9661 cggcgagata catggcaaca gttgttctat acattttcat actccttgaa tgatcgttat
     9721 ttgaacggtt ttcgttgttt cactttaggc tattttagaa agatggtcgg gccaaagaca
     9781 gcagcgtaga tgagtaaaaa cgccccgctg ccgagtatcc aaccccaatc cctttgcagg
     9841 gtgaaagggg catcgcctgt gtcctttttg cggtcaagca actgcatcat ccacgccata
     9901 cctgaggtgc cgcccatgct ggatgccaca gaagcagcca gaacaatgaa gatgccgtgc
     9961 gggttgagca gaccaatcag caaggccaca gcaccgcctg aaaaatatgc aatcagtgag
    10021 gtcatctgcg cgcggttgac ccgctccaca ccttcgccgc cgatgaaggc atccattttt
    10081 tgaatggaga ttcgaatcac tgcgtaatat cctgccagcc cgagcacggt caaagccgca
    10141 cgatacagcc actctggttc ggcttgataa aacacaccac cgctcacggt tccaaggtcg
    10201 ccgatccctg ctacacctga aaacaaaagg taccctgctg cgatcatcaa attgactgtg
    10261 gcctgatgcc acaaaaagaa tttgagtaca gcgcctgcct tcgaaaacat ttttccgaaa
    10321 agcctcagga aaaacattcc agccgccaaa ctcaccaacg gacccgccag cgccacaaaa
    10381 cgcccgccca gatctgaaac cgaatcggct gcacaatctg cataaaaagc acccatctcg
    10441 ctcaggcgcc cattgaccga gatacaagcc agcgagtgcc ccacatgctc atgaatggcc
    10501 gtggcaagca tgaaggaaaa agccgagacg gcgatcaggg tcagaatatc cacttttgtt
    10561 ttagacataa aacgctccat ccatcaaatt agaatgctgg cagtataggc gggccgcatc
    10621 aaatggtcat ctctccacag atggatttcc ccatccatct ttcgatggat ggggcgcttt
    10681 gctataatga cgcatcgcaa atcaattgtg gagaaaaaac atggaactca aagataccgt
    10741 cctcaagcgc cgcatggtgc gcaattttgc tgatacaccc gtatctcccg aaattatcga
    10801 acgcatcgtt gatctcacac gccatgcgcc cagcgcaggc ttcacacagg gacaatcctt
    10861 catcatcgtc acccagcctg acatgagaaa agccatcgcc gacacctgcg aagagggcga
    10921 atacgtcaaa agcggattcg acccattcat ctcgaaagcg cccgtgttgt taatcccctg
    10981 caccagtgag ttggcctatc accgccgcta tcaagaagca gacaaggtgg acgacaaagg
    11041 caacgagatc gtctggcctg tgccctactg gttcatggat atcggttgcg ccgtgatgat
    11101 cgccctgctc gccaccattg acgaaggatt ggtcagcgct tttgtcggct ccaaagatct
    11161 gaaaacgctg cgaacattgc tatccatccc tgaagaagtg acacccgtcg gcgtcatcgc
    11221 cgtgggctat cgcgcacctg accgaccctc accatccctc aaacgcggac ggaaacccga
    11281 tcaggattac attcaccgcg agaagtggta aaccagcctt catgcgcttc gcagaacttt
    11341 tctttttcgg cttcacgctt tggatcggtt cgtatttgct ggcacggcaa tcgcaaaaga
    11401 tcaccgtcca actgacgggc tggggcttgc tggcgtacgc gctcgcgctt gccatccaga
    11461 tcctctttga ccaatccatc ctcgtcattc tactcatccc agccatgctg tggatcggtg
    11521 cagcgctgca cctgttacca gaagaaaatc cactgcggca gatcttcatc cgcacatggg
    11581 cagtgacctc catccctgtt gtgatcctca cactggccaa tgcttggttt gcggcggtca
    11641 ttgtccttgc cctggtcatt tgcgcaggca tggttgcgca aatggcggcc aggtcacaat
    11701 tcaaaaatac gtttgcactg ctcggggtga tcgggttatt cgtcacactc agcgtgggtt
    11761 tactcatcct gccgcttaac tggattccga cttcatgggg catggtgttg cttggtttcg
    11821 atctcgtcct gctcggcatc accatcaccg cgtgggacgc cttcgacgaa ggcgaatcca
    11881 ttcgcgcgca tcttttgcgt tccttcgttt ccaatcttta ttactcaggc gcgctcgtca
    11941 tccttgcaag tctattcaac ggcagcgaca ttcttttgat cgccctgatc gcttttggga
    12001 ttctcacgca gagtttttcc aatgcgcttc aatccgtgct ggatcggttg accctgcccc
    12061 gccaaaccac agacgaacgc cagaccctgc gacaaactgc ggatgcgctg ccgcttcttt
    12121 ccgcgctgaa aactgattcg gttaccgagg agcagttcac ccgtctgacg cgacgcgcac
    12181 tctcacatct gggagatctg ccaaagctgg caaccaatcc actgacgaat ttgccgcaaa
    12241 tcaaagaatc gaatccgctc gaacgggcac atgcgttgaa atcactgctg gtgcaaagca
    12301 ttcaaaaact caaaccgcaa tcagaagcaa aatttgggac caccgatgaa tggcggtatt
    12361 tcaacgcggt ttattttccc tacgtgctcg gcctcaaacc ctatacccgc cgcgccgatc
    12421 tcgactcgct cgatgaagaa tcccgcgccg cgcttgattg gttccaaacc tccgtgcccg
    12481 aacgcacact gcacaactgg caaaacaccg ccgccaaatt gatctccgat gatataaaag
    12541 cgggccgcgt ttgaaacgcg gcccgctttt tttactgctg ggttgttgga accacgggca
    12601 gtgattgtag cttgagcatg ggggaatctg acgcgaaaga ttgccagaac ggatcgaaca
    12661 ggtcggtatt tggaatttca gcaaaaaggg tcaaaccgct tccatcgagg ttgaccagat
    12721 acaaggcctt ggttttaatt tcagtttcct tgtcatctgc actgaaaaca attcgcgcac
    12781 cgtcaggtga ccagttggca ttgtctggga tcagtgaacc tgtattcact ttgacagagc
    12841 cgctgccatc ggccttgccg atgtagatgc catccttgcg gaagaagagc acactttcgc
    12901 catcaggcga ccagcgcgga aaggcttcat tttggtcagg ggtcacctcc agcggactgg
    12961 cactaccgtc ttcgatattc atcagataaa tatcgttatc tttgagacgc agtgtgaagg
    13021 cgatttgttt tccatcagga gaccagtttg gagaatagaa atttgcttct ggttgttccg
    13081 cccgcaaagc agcggtcaat aaattttgcg tacggccatc cgaactcgaa aggatgatct
    13141 ctgaaaacgc agattcgttt cgagaatcga aggccaggtt ttgcccatcc ggcgaccaga
    13201 cagggcagcc ttcttccgcc tcgttgtctg taattcggcg tagcgtcgaa tcgctgacgg
    13261 tcatcacgaa aatatcccag cggatatcac gtttacgaaa ggcgattttg gttccatcgg
    13321 gcgaaatggc ggggcaaaat gcagaataca aaccttttgt caattttgcc atgcgcccgg
    13381 gagaggcggg gtcaagcacg tacagatcga ggccatcaag tacgttcccg ccaacagcca
    13441 tgatcaactc gttaccggca accagcccac ccgaagtcgg gagcgagcgg gaatcagggg
    13501 gaacatcgtc aaagcctttg aaattcactt ttgtattatc accacttcct gtaacagatc
    13561 cgctgcaccc ggcaaagaca ctgcccgaag catcacccac caaaaactca cccttgagca
    13621 cttcgcaatg aaccgtccca aattcggatg cattggtagc gtacaaagtg aattcggcgg
    13681 aattgcccag atccacttca atactccatg gcaatt
//
LOCUS       DAOQJC010000108        13602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2195_length_13602_cov_9.888905, whole genome shotgun sequence.
ACCESSION   DAOQJC010000108 DAOQJC010000000
VERSION     DAOQJC010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13602
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2195_length_13602_cov_9.888905"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLE39_14590"
     CDS             complement(<1..180)
                     /locus_tag="PLE39_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69610.1"
                     /translation="MFQKRIIIFANGELPNKEKARALLREDDFIIAADGGTRHAIELG
                     RTPNIIIGDLDSLPVN"
     regulatory      complement(207..314)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(345..2327)
                     /locus_tag="PLE39_14595"
     CDS             complement(345..2327)
                     /locus_tag="PLE39_14595"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNE69611.1"
                     /translation="MTTFRSRILESINDANLQTALDNNTERRLKGRAAAFESIPDWRD
                     RRLHANKIRADVIDNLDAYLEKFIAKNEANGVIVHRAKDAAEAIQIVLGIARSITNHH
                     LPTTKTLIAKSKSMVSEEIALNHALEKENMQVIETDLGEYIVQLRHERPSHIITPAAH
                     LKKEQVAELFHEKLGIPYTIDIPTLTATARKVLREVFLTADIGLSGVNFGVAETGGVC
                     VITNEGNGRMVATLPPVHIALMGMERIVPNLDDLALMLSLLPRSATGQKLTVYTQLLH
                     KPLENQTRHLVILDNGRTRLRNSPLKESLYCIRCGACLNACPVFRELGGHAYIGSDLS
                     IAPYPGPIGSVISPGLLGENYVQLAQASSLCGACKDACPVNIDLPKLLTRVRAGAVNS
                     KQSSVISEGAGLTRSTRFGLQMYTRIATHPRLFAASQKLAALTSSLFAPFSQWIHLPA
                     FTGWGYSKDFPKFAGKTFRERYVKRVASGESEINNAYSSNPQSPVTNHQLPIGRHQFT
                     EELTALGGHVYNVENATQEIIKFLKSRNINKIHLEPNLLDEGLLRESNIEFTHEPDPQ
                     ITVGVTNAICGLADTGSVLIVDGDGSPLKGSLLPEIHVAILKTSNILPSLPDAMHLVK
                     DTRAAVFITGPSRTADIEMTLTIGVHGPKEIHVFLT"
     gene            complement(2382..3107)
                     /locus_tag="PLE39_14600"
     CDS             complement(2382..3107)
                     /locus_tag="PLE39_14600"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNE69612.1"
                     /translation="MPTVQLFVTCLVDSFFPQTGEAVVDILHRLGIGVDFAPDQTCCG
                     QPQFNAGLRKDARAIAEHTIRVFENTTGDIVTPSGSCAHMFRHNYPELFEGDSLWLPR
                     AKALAERTYEFTEYLVDKLNVTDLGARWDGALTYHPSCHTLRGINVDRQPRALLAKVQ
                     GATLIDLPHAEECCGFGGIMSVEHPELSAEWLKRKISSLEASQAPVLVVTDAGCRMHI
                     AGGLNRQKKSQRVMHIAEVLNGK"
     gene            complement(3117..4229)
                     /locus_tag="PLE39_14605"
     CDS             complement(3117..4229)
                     /locus_tag="PLE39_14605"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNE69613.1"
                     /translation="MTMSHPPIKKIAISTGGGDAPGLNAVIRAAALSAIKRGWEVYGV
                     RDGFNGILSPEEYPDGGFISLTRERVRGITHLGGTILGTTNRNNPLRYPITRADGSIE
                     EVDRGDEVVRGFRLHHIDALITVGGDGSLAIASVLAKKGLRLISVPKTIDNDLDKTDV
                     TFGFDTAVGFATEALDRLHSTAASHGRVMVVEMMGRYAGWIALHSGVAGSADVILIPE
                     HPYDIHKVVEKIKEREERGSKFTIVVVAEGASPVGGAVTVVEHEAGRAERLGGIGEKV
                     AAEIHAISGKETRVVVLGHLLRGGSPTSFDRLTALRFGAAAVRALEEGQSGVMIALDP
                     PHVKYVPIEDAMGKMKMVPMDSDIVRTAYDLGVSFG"
     gene            4387..6528
                     /gene="glgP"
                     /locus_tag="PLE39_14610"
     CDS             4387..6528
                     /gene="glgP"
                     /locus_tag="PLE39_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNE69614.1"
                     /translation="MKFLTTPSQKFDIPKRIARLGELAYNLWWTWQPEAARLFGRLDY
                     DLWERLGHNPIRMLQEISHGRLVQAAKDQDYLTLYDALFESFDSYFTKSSWTQQAHPE
                     FDGNPVAYFSMEFGLHETLPIYSGGLGVLAGDHLKESSDLGLPLIAVGFMYGQGYFSQ
                     RISEDGWQEALNNPLTFEHLPVTLVTKNKKPVTIQIEFPDRTITAQIWEVRVGRVPLY
                     LLDSNVEGNTDFDRLLTARLYWSDLDRRVMQEVLLGVGGVRALRALDYSPAVWHMNEG
                     HASFLTLERVRELVTKGLPFADAAKKSLGQNVFTTHTPVPAGNDEFPLWLIDKYLANF
                     WPELGLTREEFVDIAKHTHPRGDAYSMPVLALKLSGGSNGVSELHGEVSRGMWNFLWP
                     EKQEKDVPIQHVTNGVHTANWMARRMNVLFEKYFGNDWYDHLDEPNFWSKLDSIPDQE
                     LWAVRLHLKRKLTFYMRERVRERWRQGGFHPVQVVSSGVMINPYALTIGFARRFATYK
                     RANLILSDIERLLDIINRPNMPVQIIFAGKAHPADEPGKELIQKVYRTVKKAETGGRL
                     VFVEDYDMNLARYLVQGVDVWMNTPRRPLEASGTSGMKAALNGVLNFSVLDGWWREAY
                     NGTNGWAIGEDKEYPSNEVQDAADAEDLYHTLEHEIIPLYYNRDPKEISHEWISRMKD
                     TMKTCIPQFSTRRMVKEYVEKFYAPALNRKP"
     gene            6544..6888
                     /locus_tag="PLE39_14615"
     CDS             6544..6888
                     /locus_tag="PLE39_14615"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE69615.1"
                     /translation="MFPEITVTELGEKLKTEEKFILLDIRELPELNQAKIEDSRLEVT
                     PMSRMGREGVAALSEEVRSQQVPVYVICHHGSRSVQVTGWLLQQGFKNTFNVRGGIDE
                     YARRVDPSVGFY"
     gene            7310..8929
                     /locus_tag="PLE39_14620"
     CDS             7310..8929
                     /locus_tag="PLE39_14620"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HNE69616.1"
                     /translation="MKNNPNKLWVPVFVLGWLFDFLFWKKSPGINFAIFAVSCLLVAF
                     YLLLSDGLRPNRASLILLPLFGFFAAVTFFRAEPMTTFLAVTFTLLVMTTLAMTYLGG
                     RWYLYTIGDYLGKGLQLLGSMLARPVTFTGDVRKLQAEAGIQPPKRNVWPVVRGIVIA
                     VPIIAIFSSLLSSADVVFGQRLTDLLNWFNLENLPELIFRFIYIIIIGYALAGVVLHA
                     GIESKDAKLLNEDKPFIPPFLGFVESTIVLGSVVALFAVFVVIQFQYFFGGQANINIE
                     GYTYSEYARRGFGELVTVAFFSLVMLLTLTSITRRETEAQRRIYSGLGVALVVLLLVM
                     LFSAFRRLGLYEAAYGFSRLRTYTHVFLVWIGLLLVVTIVLEILHKERMFTFAALVAS
                     FGFAISLPILNVDAFIVEQNIRRGDLQAQTEASVSRVQEEEVDAQYFVELSDDAVPAL
                     VKAYLNPKLPDPVHEKIGAALACINQQRTYDGGRESSWQSFHFSSFNADRALASISEE
                     LKNYEVVDLDFPPTVILPSGEDFPCSTYYFD"
     gene            9057..9614
                     /locus_tag="PLE39_14625"
                     /pseudo
     CDS             9057..9614
                     /locus_tag="PLE39_14625"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
     gene            complement(9738..10553)
                     /locus_tag="PLE39_14630"
     CDS             complement(9738..10553)
                     /locus_tag="PLE39_14630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNE69617.1"
                     /translation="MFDLSNARFAFLDIETTGLSPWFGDRICQIAIVLTEGKRIKSTL
                     DLLINPGRGLSPAASHINGLDESKLSAAPMFEEITDQLEAALKDAVVVCHNAKFDIQF
                     LDNEYRKLGRSIEYPNLIDTLLLAREYFDLPSYSLHHLAEDFSISRNSQGSRAMADAL
                     TAKNLFFAMMDTLKPNGKPLDDYIGIYNSPAWPEEGVYLPTELSEAIHSGRRLFIKYM
                     DKDGEISERWISPNQVIGLADYIYLQAYCHTREAERTFRLDRIIEVAMEIEKE"
     gene            11030..13009
                     /locus_tag="PLE39_14635"
     CDS             11030..13009
                     /locus_tag="PLE39_14635"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNE69618.1"
                     /translation="MKKTMYWLTSVIVTITLTLGSFSAVGASPLAASKTYYVSLTGSD
                     TNAGTATAPFKTFTKAVSMLVAGDVLQVYGGTYTQQLNVKKSGTAAAPITVQPVAGQK
                     VIIDGGMTLDRDIILSGSYINISGFETVSATDFCVDVTGSNISVKNFYIHNCFRFGAR
                     IKGKYVTFEGSTLHSNVLENTGGVNTTGGWAGTLRVGLGGENITLKNNTIYNNYGEGI
                     GVGQGRFVNIYNNVVHDNYSQNIYVNNSTDVNVENNFVYSVDPTYYRSGAPANCIGMA
                     DEYYSGWGAQLARVRVINNIAAFCKRGIGYTYSENTDGGMDTVTIAYNTVWGSTEAGI
                     VAINQPTKTRNSVISNNIVQQSDGRLTDIQVGVGISYSNNFWVSPAKTFKSGSGDKTG
                     DVKLAAAPGLTFESFRLSSSSPAIAGAVSMGIAQDFAGNSRGPSYDMGALQFTGIVSA
                     TATAAPALPTATQPIGMTATPTRQVTATSTPIWGFPTAVPSATKTATPVVVPSTATAT
                     PIVQVSPTFTPTGVVPTATVPVSTQPSSETVFDDKNGNLVYSVGWSDVAAKQAYGGSY
                     KQTTQSGSTVSITFTGQSFSVLYKGGPSYRKMDVFVDGALVATLDENLTVSTYQPRWD
                     YPGQLAAGTHTLMLVFVSTPGVANNNGSVDAVIVR"
     gene            complement(13090..13413)
                     /gene="trxA"
                     /locus_tag="PLE39_14640"
     CDS             complement(13090..13413)
                     /gene="trxA"
                     /locus_tag="PLE39_14640"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNE69619.1"
                     /translation="MTDLQYVTEADFQEEVVGSTMPVLVDFTATWCQPCKMIEPIVKQ
                     LAEEWDGKVKVVKLDADQNPNVLMQYGVMGIPTLMLFKGGEIKERLTGFQPKEKLAAK
                     VTPHV"
     gene            13507..13578
                     /locus_tag="PLE39_14645"
     tRNA            13507..13578
                     /locus_tag="PLE39_14645"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13539..13541,aa:Lys,seq:ttt)
BASE COUNT         3109 a         3517 c         3505 g         3471 t
ORIGIN      
        1 gttgacaggc aaagaatcaa ggtcgccaat gatgatgttg ggtgttcttc ccagttctat
       61 ggcatggcgt gttccgccgt ctgcggcgat gatgaagtcg tcttcacgca gcagggcgcg
      121 ggctttttcc ttattgggca attcgccgtt ggcaaagatg atgatgcgtt tttgaaacaa
      181 aaaatcctcc gaaggcggag gatggctata ggcgagtcaa gagtccctcc gctggtatta
      241 accagatcag gtaatgcggg tcgagcgaag tacgctcctc tcagcctttc gaaaaggctc
      301 cccgttcaat tgtgaagtca gtatatgtcc gcgatgggcg gatgtcaagt caaaaaaacg
      361 tggatctctt tcgggccatg cacaccgatg gtcagggtca tttcaatatc agccgtgcgc
      421 gatggccctg taatgaagac tgcggcgcgt gtatctttca caagatgcat ggcatcgggc
      481 aaagatggca gaatattgga tgtcttcaaa atggcgacat gaatctcagg aagcaacgac
      541 cccttgagcg gactgccatc cccgtccacg atcaaaacgg acccggtatc tgccagcccg
      601 caaatggcat tcgtcacccc gacagtgatc tgcgggtcgg gttcatgggt aaattcaatg
      661 tttgattcgc gcaaaagacc ttcgtccagc agatttggct caagatgaat cttgttgatg
      721 ttgcgcgatt tcaaaaactt gataatttct tgcgtggcat tctcaacgtt gtagacatgc
      781 ccgcccagtg cggtgagttc ttcggtgaat tgatgcctgc caatgggcaa ttggtgattg
      841 gtaactggag attgaggatt tgacgagtat gcattattaa tctctgattc tccgcttgct
      901 actctcttca catatctttc tcgaaacgtc ttccctgcaa attttggaaa gtccttgctg
      961 tagccccagc ctgtgaacgc gggcaggtga atccactgcg agaacggggc gaagagagag
     1021 gatgtcagcg cagccaattt ttgtgacgcc gcaaacagcc ttgggtgagt cgcgatgcgg
     1081 gtgtacatct gcaaaccgaa tctggtcgag cgagtcaatc ctgcgccttc actgatcact
     1141 gacgactgtt tactgttcac tgctcctgcc ctcacacgag tcaataattt cggcagatca
     1201 atattcaccg ggcaggcgtc tttgcacgcg ccgcacaatg acgaagcctg cgcgagttgg
     1261 acataatttt cgccgagcag cccaggcgaa atgaccgagc caatggggcc aggatacggc
     1321 gcaatggaaa ggtcactgcc gatgtaggca tggccgccga gttcacgaaa aacgggacag
     1381 gcgttcaagc acgcgccgca gcggatgcaa tacaacgatt ctttgagcgg cgaatttcgc
     1441 aggcgtgttc gcccgttatc aagaatgaca agatgacgag tttgattttc gagcggcttg
     1501 tgtaacagtt gggtataaac ggtgagtttt tgccccgtgg ccgagcgggg gagtagtgag
     1561 agcatgagcg cgagatcatc aagatttggg acaatgcgtt ccatgcccat cagcgcaatg
     1621 tggacaggcg gcagggtggc gaccatgcgt ccgttgcctt cgttggtgat gacacatacg
     1681 ccgcccgttt ctgctacacc aaagttgact ccgctcaagc cgatgtcggc ggtgaggaaa
     1741 acttcgcgca gcactttgcg tgcggtggcg gtgagggtgg ggatatcgat ggtgtaggga
     1801 atgccgagtt tttcgtggaa gagttcggcg acctgttctt ttttgagatg cgccgcaggg
     1861 gtgatgatgt gacttggccg ctcgtgccgc aattgcacaa tgtattcgcc aagatcggtt
     1921 tcgatgacct gcatattttc tttttccaat gcatggttga gcgcgatctc ttcactgacc
     1981 atgcttttgg attttgcgat gagagtcttg gtagttggta agtggtgatt ggtaattgac
     2041 ctggcaattc ccaacacaat ttgaatcgcc tctgccgcat cttttgcgcg gtggacaatg
     2101 actccattgg cctcgttctt tgcgatgaat ttttcaagat acgcatcaag gttgtcaatc
     2161 acatccgcgc ggatcttgtt tgcgtgtagc cgcctgtcgc gccaatcagg gatggattca
     2221 aatgcggcgg cgcggccttt caaacggcgc tcggtgttgt tatccagcgc agtttgcagg
     2281 tttgcgtcgt tgattgattc gagaatacga gagcgaaatg tagtcatagg agtttgaacg
     2341 tttaaacgtt gtaacgttgg aaccttctaa cgtcggtata ttcactttcc attcaacact
     2401 tcagcgatat gcatcacgcg ctgacttttc ttttgacgat tcaacccgcc cgcaatgtgc
     2461 atgcgacaac ccgcatcggt cacgactaaa acgggagcct gactcgcttc caagctgctg
     2521 atcttgcgct ttagccattc tgctgaaagt tcagggtgct ccacagacat gatgccgcca
     2581 aagccgcagc attcttcggc gtgcggaagg tcgatcagtg ttgcgccttg gacttttgcc
     2641 agtaacgcgc gcggctgacg atccacattg attccgcgca gggtgtggca ggatggatgg
     2701 taggtgagtg ctccgtccca ccttgcgccg agatcggtga cgttgagttt atctacaaga
     2761 tattcggtaa attcatacgt gcgttctgcc agtgcctttg cgcgtggcag ccagagcgaa
     2821 tcaccttcaa agagctcagg gtaattatgg cggaacatgt gcgcgcacga acctgatgga
     2881 gtcacaatgt cgcctgttgt gttttcaaag actcggatgg tgtgttctgc aatggcgcgc
     2941 gcatccttgc gaagccctgc gttgaattgc ggctgtccgc aacaggtttg gtcgggggca
     3001 aaatccacgc caatgccgag gcggtggaga atatccacga cggcctcacc agtttgcggg
     3061 aagaaagaat cgacgagaca ggtgacgaag agttgaacgg tgggcatgaa tccgttttac
     3121 ccgaacgaaa ccccgagatc atacgccgtg cggacaatgt cagagtccat tggcaccatc
     3181 ttcatcttgc ccatggcatc ttcgatcggt acgtatttga cgtgcggcgg gtcgagggcg
     3241 atcatcacac cactctgacc ttcttccaat gcgcgcactg cggctgcgcc gaatcgcagt
     3301 gcggtgagac ggtcaaagga agtgggggag ccgccgcgca ataaatgacc aagcaccaca
     3361 acgcgggttt ccttgcctga aatggcatga atttctgccg ccactttttc gccaatgccg
     3421 cccagccgtt cggcgcggcc cgcttcatgc tcaaccacgg tcactgcacc gcccaccgga
     3481 gatgcgcctt cagccaccac cacgatcgta aatttgctgc cccgctcttc gcgctccttg
     3541 attttttcaa cgactttgtg aatatcgtag ggatgttcag gaatcaagat cacatctgcc
     3601 gaaccagcca cgcctgaatg caaagcgatc cagcctgcgt agcggcccat catttccacc
     3661 accatcaccc gtccatgcga ggcggctgtg gaatgcaggc ggtcaagcgc ctcggtggcg
     3721 aatccgaccg cagtatcgaa accaaaggtg acatcggtct tatcgaggtc attgtcgatc
     3781 gtttttggca cagagatcag acgcaggccc tttttggcga gcacacttgc aattgccagt
     3841 gagccatctc cgccgaccgt gatgagcgcg tcaatgtggt gcaagcgaaa gccgcgcacc
     3901 acttcatcgc cgcgatccac ttcttcgatg cttccatctg cgcgggtgat cggatacctg
     3961 agcggattgt tgcggttggt cgttcccaga atggttccgc ccaaatgggt aattccgcgt
     4021 acccgctcgc gtgtgagtga aatgaacccg ccatcggggt attcttctgg tgagaggatt
     4081 ccgttgaaac catcgcggac tccgtacact tcccagccgc gtttgatggc tgaaagggct
     4141 gctgcacgga tcactgcatt caaaccgggg gcgtcgcctc caccggtgct gattgctatt
     4201 tttttaatgg gaggatgaga catggtcatg aggttgctcc agtaaatata gactttctgg
     4261 ttggattata gtcctgttga cagagtgtat gccatgtgaa aatctacact ttgttcacat
     4321 tacaatttat gcaggaaaac aaatccgtgc aacttcgcgt catttttacg gtatcattct
     4381 gccgctatga aattcctaac caccccctcc caaaaatttg atatccccaa gcgcattgcg
     4441 cgcctcggtg aattagccta caacctgtgg tggacgtggc agccagaagc tgcccgcctc
     4501 ttcggacgac tcgattatga cctgtgggaa cgcctcggcc ataatcccat ccgcatgctg
     4561 caagagatca gccacggtcg tttggttcaa gccgcgaaag accaggatta cctcaccctg
     4621 tacgatgccc tttttgaaag ttttgattct tatttcacga agtcatcctg gacccaacag
     4681 gcccaccccg aattcgatgg caaccctgtg gcctatttct ccatggaatt tggtttgcac
     4741 gagaccctgc ccatttattc gggcggcctt ggcgtactgg caggcgacca cctgaaagaa
     4801 tcctccgatc ttggcctgcc attgatcgct gttggattca tgtacgggca gggatatttc
     4861 tcccaacgca tcagtgaaga tggctggcag gaagcgctca acaatccgct cacctttgaa
     4921 cacctgcctg tcacacttgt taccaaaaac aagaaacctg tcaccattca aatcgaattt
     4981 cccgaccgca ccatcaccgc ccagatctgg gaggtgcgtg ttggccgtgt gccgttatac
     5041 ctcctcgatt caaacgttga aggcaacacc gattttgacc gtctgctcac cgcccgcctg
     5101 tactggtctg accttgaccg ccgtgtcatg caggaagtat tgctcggcgt agggggcgtg
     5161 cgtgccttgc gcgcgctgga ttattccccc gcggtctggc acatgaacga aggccacgca
     5221 tctttcctga cccttgagcg ggtgcgtgaa ctcgtgacaa agggcctccc ctttgcagat
     5281 gccgccaaaa aatccctcgg acagaacgtc ttcaccaccc acacacctgt ccctgcaggc
     5341 aatgacgaat tcccgctctg gcttattgat aaatacctcg ccaacttctg gcccgaactt
     5401 ggcctcaccc gcgaagaatt tgttgatatt gccaagcata cccatccgcg cggtgatgcc
     5461 tactccatgc ctgtactggc tttgaaactg tctggcggca gcaacggcgt ttccgaattg
     5521 catggcgaag tctcccgcgg catgtggaat ttcctctggc ctgaaaagca ggaaaaggat
     5581 gtccccatcc agcatgttac caacggcgtc cacaccgcca attggatggc acgccgcatg
     5641 aacgttcttt tcgaaaaata tttcggcaac gattggtatg accatcttga tgaacccaat
     5701 ttctggtcga agcttgattc cattccagat caggaactgt gggcggtgcg tttgcacctc
     5761 aaacgcaagt tgacttttta catgcgtgag cgtgtccgcg agcgctggcg tcagggtgga
     5821 ttccaccccg tgcaggtggt ttcctccggt gtgatgatca atccctacgc attgaccatc
     5881 ggttttgccc gccgtttcgc cacctacaag cgtgccaatc tcatcctttc tgatattgag
     5941 cgcttgctcg atatcatcaa tcgcccgaac atgcctgtgc agatcatctt tgccggcaaa
     6001 gcgcaccccg ccgatgagcc tggcaaggaa ttgatccaaa aggtctatcg taccgtcaaa
     6061 aaagccgaaa ccggcggacg cctcgtcttc gtggaagatt acgacatgaa ccttgcccgc
     6121 tacctcgtcc agggcgtgga tgtgtggatg aacacccccc gtcgtccgct cgaagccagc
     6181 ggcacctcag gcatgaaagc cgccctgaat ggcgtgctga atttctccgt gcttgatggc
     6241 tggtggcgcg aagcctacaa cggcaccaac ggctgggcca ttggcgaaga caaggaatac
     6301 ccctcgaacg aagttcagga tgccgccgat gcagaagacc tctatcacac cctcgaacat
     6361 gagatcattc cgctctacta caaccgtgac ccgaaggaaa tttcacacga gtggatcagc
     6421 cgcatgaaag acaccatgaa gacctgcatt ccgcaattct ccacccgccg catggtaaag
     6481 gaatacgtgg aaaaattcta cgctcctgca ctcaatcgta agccgtaaat ctttagaggt
     6541 cccatgtttc ctgaaatcac cgttaccgaa cttggcgaaa agctcaagac cgaagaaaag
     6601 tttatcttgc tcgacatacg cgaactaccg gagctgaatc aagccaaaat cgaagacagc
     6661 aggcttgagg tcacgccgat gagccgtatg ggtagggaag gggtggcggc tctctccgaa
     6721 gaagtccgct cacagcaggt acccgtctac gttatatgtc atcatggcag ccgaagtgtg
     6781 caggtgacag gctggctttt gcaacagggt ttcaaaaata cctttaacgt ccgcggcgga
     6841 attgatgagt atgcccgtag ggtggaccct tctgtaggtt tctattaatc ccaaaagggc
     6901 acagtcacac tgtgcccttt ctgttttaaa gccaaactgc cacccttgcg gatggcagtt
     6961 tggggtttgc gctgtggcgt cctttagatc ttgaggaggg atgccacagc gctgggttta
     7021 ctactactat tcataagcga catcacctcc tcctttcatt aggatggcaa ttcaatttgt
     7081 ttgcccccat ccagtgtcga aagtatgtca taaaataaaa tgaatgtcaa tagggcattt
     7141 ttgattctca caaatttgta atattaaatc ggcgcttttt ggcaaaatat gatacctttt
     7201 gagagtctcc gccagacttt cacggctatt ctgttcccat tgcgcgggtc ataccgaggc
     7261 atattcaaat catcgtcctg agttacggca aaggacgtta ggagaactga tgaaaaacaa
     7321 tccgaataaa ctgtgggttc ctgtatttgt acttgggtgg ctgttcgatt tcctgttttg
     7381 gaaaaaatca cctggcataa attttgccat ttttgcggtt tcgtgtctgt tggtggcgtt
     7441 ctacctgctt ttgagcgacg ggcttcgccc gaatcgcgcc agcctgattc tgcttccgct
     7501 ctttggcttt ttcgccgctg tcacattttt ccgcgccgag ccaatgacca ccttcctggc
     7561 cgtgacattc acccttttgg tgatgaccac cctcgcaatg acctaccttg gcgggcgttg
     7621 gtatttgtac acaattggtg attatcttgg caaaggcttg cagctgcttg gaagcatgct
     7681 ggcacgcccc gtcaccttta caggggatgt gcgcaaattg caggccgaag cagggattca
     7741 acccccaaag cggaatgttt ggccggtggt gcgcggcatt gtcattgctg tgccgatcat
     7801 tgccattttc tcttccctgc tttcttcagc tgatgtcgtc ttcggtcaac ggctgactga
     7861 cctgctcaac tggtttaacc ttgaaaacct gcctgagctg atcttccgct ttatctatat
     7921 catcatcatc gggtatgcgc tggctggggt ggtcttgcat gcggggatcg agtcgaagga
     7981 tgcgaaactt ctcaatgaag ataagccctt cattccgcca ttccttggct ttgttgaatc
     8041 aaccattgtg ctgggcagtg tcgtggccct gttcgctgtg tttgtcgtca ttcagttcca
     8101 gtattttttt ggcggacaag ccaacatcaa cattgaaggc tatacctact ctgaatacgc
     8161 ccgccgtggc ttcggtgaac tggtgacagt ggctttcttc tcactggtga tgctgttgac
     8221 acttacctcc atcaccaggc gcgaaaccga agcacaacgg aggatctact ctgggttggg
     8281 ggtggcgttg gtggtgctcc tgttggtcat gctcttttcg gcgttcaggc gtcttggcct
     8341 gtatgaagcg gcatatggat tttcacgcct gcgcacatac acccatgtct tccttgtctg
     8401 gataggttta ttgctcgtcg tcaccattgt gcttgaaatt ttgcacaagg agcgtatgtt
     8461 cacctttgcg gccctggttg cgtcgtttgg gtttgcaatt tccctgccca tcctcaatgt
     8521 ggatgcgttt atcgtggagc aaaacattcg gcgtggtgat ttgcaggcgc aaaccgaagc
     8581 ctccgtttca cgggtgcagg aagaagaagt ggatgcccaa tattttgtgg agctttctga
     8641 cgatgccgtt ccggcgcttg tgaaagccta cttaaacccc aaactgcctg accctgtgca
     8701 tgagaagatc ggcgcggcgc tggcttgtat caaccagcag cgcacctatg acggtggacg
     8761 tgaatcttcg tggcaatcct tccatttctc ctcgttcaat gctgatcggg cattggcttc
     8821 gatctcagag gaattgaaaa actacgaggt tgtcgatctg gatttccccc ctacggtcat
     8881 tttgccctct ggcgaagatt ttccctgttc cacctattat tttgattagt ttgtaagaaa
     8941 ccagtctcat tgtcagtcta ttcacagaat atccaaacct aaacgagcca aaggaactga
     9001 caatggactt caagctgacc atcttttctg attacatctg accgtggtgc tatgtcggcc
     9061 agggtgtggt cgaccaactg aaagctgaat acaacgtgga tgtggagtgg cgtccgtttt
     9121 acctgcgccc cgataccccg cctgaaggca tggacctgcc cgattacatt ctccgtgcgc
     9181 gggccaatgg cgccgatgaa cgtttgcaaa gcatggcgaa aatgcatggc atgaacttca
     9241 actcgccaga ccgcatttac aatacccgcc ttgcccatga agccactgaa tatgcccgtg
     9301 aacatggcaa agcactcgaa ttccatcgcg tggtttttcg caaggtgtat ggtgaagccc
     9361 tcgatatcag caaatgggaa atactccgtg ccgctgccga agaggttggc ctcaatgggg
     9421 aagatatgca aactgttgta gaaggcggaa aatacaccgc tgaagtggcg gggcaggttc
     9481 aatatgccca gcagatcggt gtatccggtg tgccgactta tgtcatcaac gagcgttatg
     9541 ccatcgttgg cgcccagccg tatcaggcct tcaaaaatgc cctgacgcgg atcttccaag
     9601 agaagaaaga ttaaaatcaa gacaaaataa tactcaccac gaaggacaac tttcgtggtg
     9661 agtatgtttt taagaggctt ttgccagcca cctgcttatt tcattttttg gtgaatcgca
     9721 tagcgaagca atctttttta ttccttttcg atttccattg ccacttcaat aattcggtct
     9781 aatcgaaacg tacgctcagc ttcacgggtg tggcagtagg cttgcaggta aatgtaatcg
     9841 gccagcccga tgacttgatt gggggagatc cagcgctctg aaatttctcc gtccttatcc
     9901 atatatttga tgaatagccg cctgccgctg tgaatggctt cgctcaactc ggtgggcagg
     9961 tatacgcctt cttccggcca ggcaggggag ttgtaaatgc cgatgtaatc atcaagtggt
    10021 ttgccatttg gcttgagcgt atccatcatg gcgaagaaga gatttttggc tgtgagagca
    10081 tctgccatag cgcgcgagcc ttgtgaattc cgtgaaatgc tgaaatcttc ggcaaggtga
    10141 tgcaggctgt aggaaggcag gtcaaagtat tcgcgggcca gcaacagggt atcgatcaaa
    10201 tttggatatt cgatggaacg tcccaacttg cggtattcat tgtccaggaa ttggatatcg
    10261 aatttggcat tgtgacagac aacgaccgcg tcttttaacg ccgcctcaag ctggtcggtg
    10321 atttcttcga acatgggtgc cgctgaaagt tttgactcat ccaacccgtt gatgtgagag
    10381 gctgccgggg agagtccccg cccggggttg atgagcaggt caagggtcga tttgatgcgt
    10441 ttgccttcgg tgaggacaat ggcgatctgg cagatccgat ctccgaacca tggggaaagt
    10501 ccggtggtct cgatatccag aaaagcgaag cgtgcattgg aaagatcaaa catgagttgg
    10561 gggaaccgcc tttttgatgt gggcttatct taccgctttt tttcgtttgg ggaaatcgta
    10621 actttttcct gtttttagcg accctgtaca taccaagtcg ctgatagaga caaaaaaatc
    10681 gtttcaaaaa gtatttaaaa aattgatttt taagtttaaa ttttccgtcc tttccatcac
    10741 ctttcaggat ttgggggagt gtctttggca ttttttgcca ttatgagcat tggataagct
    10801 gtttcttgct gatttgtaaa gtgactttgc ctgtttacag aaatgtgcag atttctagta
    10861 ctcaggtcat ggctgggaga cccttgcacg ggagaccgac atgttaatct ttatgcacgc
    10921 aaatataccg tttgcctttt gcgaacaaac gctgcaacga agcggcaaat ctgacaaaag
    10981 cctgaacgca aagaatacag gccaacggtg taacgaagga gatatcgtaa tgaaaaagac
    11041 aatgtactgg ctgacgagtg ttatcgtaac catcaccctc acacttggaa gctttagcgc
    11101 ggttggggct tctcccctgg cagcatcaaa gacctattat gtttcactga ccggcagcga
    11161 caccaatgca ggaactgcca ccgctccgtt caaaacgttc accaaggccg tttccatgct
    11221 tgtggctggt gacgtgctgc aggtttatgg aggtacgtac acccagcaac tcaatgtgaa
    11281 aaagagcggc acggctgctg cgcccatcac tgttcagcct gtggctgggc agaaggtcat
    11341 cattgacggc ggtatgaccc ttgatcgtga cattattttg tctggcagct atatcaatat
    11401 cagcggcttt gagaccgtga gcgccaccga tttttgtgtg gatgtgactg gttcgaatat
    11461 ttcagtgaag aatttctaca ttcacaactg ttttcgcttt ggtgcgagaa ttaagggcaa
    11521 gtatgtgacc tttgaaggtt caacccttca cagtaatgtg ctggagaaca ctggcggcgt
    11581 aaataccact ggcggctggg ctggaaccct gcgggttggc cttggcggtg agaatatcac
    11641 cctcaaaaat aacaccatct ataacaatta cggcgaagga attggcgttg ggcagggacg
    11701 ctttgtgaac atttacaaca atgtcgtaca tgacaactac tcgcagaata tttacgtcaa
    11761 caattcgacg gatgtgaacg ttgaaaataa cttcgtctac agcgttgacc ccacctatta
    11821 ccgcagcggt gcccctgcga actgcattgg catggcagat gaatattaca gcggctgggg
    11881 tgcgcagctg gcccgcgtgc gcgtgatcaa taacattgcc gcattttgca agcgcggtat
    11941 tggttatacc tattccgaaa ataccgatgg cggcatggat actgtgacga ttgcctacaa
    12001 caccgtttgg ggcagcactg aggcaggtat cgtcgccata aatcaaccca ccaagacccg
    12061 caactcggtc atttctaata atatcgtcca acaatcagat gggcgtttga cagacattca
    12121 ggtcggtgta ggaatcagct atagtaacaa cttctgggtc agcccggcca agaccttcaa
    12181 gagcggatct ggcgacaaga ccggtgatgt aaaactggcc gcggctccag gcctgacctt
    12241 cgagtcgttc cgcctgagca gcagttcacc tgccattgca ggagcggtct caatgggcat
    12301 cgcccaggat ttcgctggaa actcacgtgg accttcctat gatatgggtg ccttgcaatt
    12361 cactggtatc gtttctgcca cagctacggc tgccccggca ttgccgaccg ctacccagcc
    12421 gattggtatg accgccaccc caaccagaca ggtaactgcc acctctactc caatttgggg
    12481 ctttcccacc gctgtaccgt ctgccacaaa gaccgccacc ccagttgtag ttccttcgac
    12541 agccactgcc acacccattg ttcaagtttc acccaccttt acccccactg gtgttgtacc
    12601 taccgcaacg gtgccagtct ctacacagcc atcgtcagaa acagtgttcg atgacaagaa
    12661 tgggaatttg gtctattctg tgggttggtc agacgtagct gcaaaacagg cttatggtgg
    12721 ttcctataaa cagaccactc aaagcggatc aacggtttcc atcacgttca cgggacaatc
    12781 cttcagcgtt ctttataagg gcggaccgtc ctatcggaag atggatgtat ttgtagatgg
    12841 tgctctggtg gctacactgg atgaaaacct gaccgtctcc acctatcagc cgcgctggga
    12901 ttaccccggc caattagcag caggcaccca cactttgatg ctggtttttg tcagcacacc
    12961 cggtgttgcc aataacaacg gctctgtcga cgctgtgatc gttcgatagc aaaaaaaaca
    13021 gttcagccga tgcccgactg aactgtaata tctaaaacaa aaagagccag gtcactggct
    13081 ctttttgttt tagacgtgcg gtgtaacttt cgccgccagt ttttccttgg gctgaaatcc
    13141 tgtcagacgt tccttgatct cgccgccctt gaacagcata agggtgggga tgcccatgac
    13201 accgtactgc atcaggacgt tcggattctg gtctgcatcg agtttcacta cctttacctt
    13261 gccgtcccat tcttcagcca gttgcttgac gatgggttcg atcattttac agggctggca
    13321 ccaggtcgct gtgaaatcca caaggacggg catggtggaa ccaaccacct cttcttgaaa
    13381 atctgcctca gttacatatt gtaggtctgt catttttgcc tccagcggca tagacgttat
    13441 gtcgaatttc aatattacgt tgacggtctt aatttcacgt gataaaatgc agtccgtttt
    13501 agtaatgggc gcgtagctca atggcagagc agtggccttt taagccattg gttaagagtt
    13561 cgaccctctt cgcgctcaca aatgccctcc tcattggagg gc
//
LOCUS       DAOQJC010000109        13241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2292_length_13241_cov_9.121872, whole genome shotgun sequence.
ACCESSION   DAOQJC010000109 DAOQJC010000000
VERSION     DAOQJC010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13241
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2292_length_13241_cov_9.121872"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLE39_14650"
     CDS             complement(<1..242)
                     /locus_tag="PLE39_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69620.1"
                     /translation="MNKHRSFFELPNIKKEIIPVASGIPFGFSTVYNPALGIGGVYGA
                     WGDSYDNHSLRDLVAQRYGEPLDDEDIMNLAELGFL"
     gene            468..962
                     /locus_tag="PLE39_14655"
     CDS             468..962
                     /locus_tag="PLE39_14655"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNE69621.1"
                     /translation="MMMTTTTTMETLAIKTENKFRTLVTKQSYKKGEMICAMPSENIT
                     DRPNRFTVQIGREVHTHVGKLAALNHSCDPNVILDTKKMVMVARRDIEKGEELSFFYP
                     STEWEMQAPFICICGSANCIHVVAGARFLPLSTLENHYLNPHIREMMIELLNNTQEHL
                     KSLK"
     gene            complement(1035..1457)
                     /locus_tag="PLE39_14660"
     CDS             complement(1035..1457)
                     /locus_tag="PLE39_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69622.1"
                     /translation="MSRVINPDSVGKERTRLTKSIVLCIREMMKMSQVTPEAKDMASF
                     IALALQSISDGVDASVAAWEKRDYWVKADKFRMEWRWTGQLADKMKSAVLGDDWATIA
                     SLLPQIAMKLNKIEVSDNHRLGKPWENAYALLALHRKL"
     gene            1590..1671
                     /locus_tag="PLE39_14665"
     tRNA            1590..1671
                     /locus_tag="PLE39_14665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1624..1626,aa:Leu,seq:tag)
     gene            1724..3109
                     /gene="tig"
                     /locus_tag="PLE39_14670"
     CDS             1724..3109
                     /gene="tig"
                     /locus_tag="PLE39_14670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNE69623.1"
                     /translation="MNIEKQIQEDHSAKLIVDVEQEKMDVYKRRAATKISARSKIPGF
                     RPGKAPYDLVVRTFGEGAVTEQAIDLFIDAEYGNLLKEADVNPGAAGSLESIDSLTPP
                     KLTFRVPLAPEVDLGTYHDIRMEYEWQAPDQAAVDAALEDLRQMYASTETVDRAIELG
                     DYVLVDVNSETAEVTRKGFATFVRKEDRDTEWPYNGFAKELIGLKAGESKTIQHTFPA
                     DWELEELRDKEVTMEVTVKTVRGVTLPDIDDAFAKMTGAGETVDELKEAVKKDVEARS
                     QAEYDDKYFVELIEKIKEVATIKYHEHTLEHEGEHVLSDLSNRLAQQNMDLDTYFKMR
                     GTTREQFIQDEVNPVAKKRLERGLILDEIVRQEKIQIDNEALEAEYNVTLNGLAQQGM
                     DFSKIRGGRKGQKQLSEAIAMESASRVMTRKALDMIKSIATGEYKPIEERQAEASPEA
                     GEASETESKPE"
     gene            3205..3813
                     /locus_tag="PLE39_14675"
     CDS             3205..3813
                     /locus_tag="PLE39_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNE69624.1"
                     /translation="MIPDFKNVVPMVVENTGRGERAYDIYSLLLKERIVFVGTPIDDQ
                     VANVIVAQLLFLNHEDPEKEIRMYINSPGGQIYAGLAIYDTMQIISNPISTVAVGVTA
                     SFGTVLLTAGTKGRRYALPHATVHMHQPLGGAQGQATDIEIQAKQILRLKALLNGVLS
                     SHTGQPLEVIERDADRDFYLDAQQAVDYGLIDQVIEMPVKSK"
     gene            3954..4799
                     /locus_tag="PLE39_14680"
     CDS             3954..4799
                     /locus_tag="PLE39_14680"
                     /inference="COORDINATES: protein motif:HMM:NF018024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HNE69625.1"
                     /translation="MTFSIVACDLKEKTWGIAVASKFPAVGAVVPWAAAGAGAVATQS
                     FANTSFGPRGLEMMAAGLSADETLAKLLMDDPDRELRQVGLVDANGKSATYTGSGCFP
                     WAGGVNGKGYAIQGNILANAKVVPAMEKKFLATKGDLPTRLHAALLAGDRAGGDKRGR
                     QSAAIYVVKPNGGYGGYVDRWIDYRVDDHEDPVPRLGELLELHRLYFDKSPENERAAL
                     KGKTLEQMTSILKRAGYLLKKNTAFVDAFNAFIGNENFEERADPNGQWIDKPVLKYLV
                     KKFGK"
     gene            4855..5640
                     /locus_tag="PLE39_14685"
     CDS             4855..5640
                     /locus_tag="PLE39_14685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNE69626.1"
                     /translation="MIPSNIKALILDMDGVIWKADAPIGDLPSTFNRIRERGLKFVFA
                     TNNGTKTPEDYQQKLADLGVDIAPSQVVTSAMGIAFMLAQRFPRGTKIFMIGEDGIRV
                     ALEEKGFEILSAENASQAQAFVMGIDRGINFQKVAEATLLVRAGIPFYTTNTDKTFPT
                     PRGEIPGSGAWVSVIQTATNVEPIIAGKPFPFLMELSLEKLGTKKEETLVVGDRLETD
                     IAAGQSVGCPTALVLSGVSTKAQADAWTPKMDIIAASLADLVK"
     gene            complement(5750..6463)
                     /locus_tag="PLE39_14690"
     CDS             complement(5750..6463)
                     /locus_tag="PLE39_14690"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNE69627.1"
                     /translation="MNLKNFDINALKSRPAILWGLVAVLVLAGICTLVYAFRGGKSEQ
                     ASEGDINVIYTNAAATVNAGQTAIALSVTPTATLNTALLVPSPTVAPLATVTTQPLTI
                     LATATKAVPTSGCDMSIYVSDVTVPDGTAMTAGQTFTKTWKVTNTGACTWTATYKLVF
                     VAGDGMNGQSTPIGKEVKPGESVDISVNLTAPAKSGEIKGTWRLSNDKSVAFGDSLTV
                     VIKIGTVPTPTKTPGGYPN"
     gene            6636..7706
                     /gene="rlmN"
                     /locus_tag="PLE39_14695"
     CDS             6636..7706
                     /gene="rlmN"
                     /locus_tag="PLE39_14695"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNE69628.1"
                     /translation="MLIYDFDQKTLLDLLTRWGEPAYRAKQIWQGLYQHLYSSPEQFT
                     NLSKPLRDKLAEGLNFAPLKPKLYLDSSDGQTRKTLFELGDGQVIESVLMRYGDPADN
                     HDTSGRDIRSRRTLCISTQAGCAMGCVFCATGQMGFKRHLSSGEIVAQVMYYAQMLKE
                     ENLSVTNIVFMGMGEPFHNYENMMAAIDRLNDADGFNFGARRMTISTVGLAPQIKRFA
                     DEHRQVNLAISLHAADDDERLAIMPVNKRYKIADVMEACKYYVEKTHRRVTFEWALIN
                     GVNDTPETAKKLAARLRGLLCHVNAIPLNPTTGYEGEATSRERANLFKESLEQAGVPC
                     TIRMRRGIDIAAGCGQLAGSVN"
     gene            7877..>11232
                     /locus_tag="PLE39_14700"
     CDS             7877..>11232
                     /locus_tag="PLE39_14700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HNE69629.1"
                     /translation="MEQKQNWLERPIHPALPGLTNEIAIFIGVILLAIITRFYDLGTR
                     VMSHDESLHTYFSWLLYRGQGYEHSPMMHGPFQFHIVALTYFLFGASDFTSRIPAALF
                     SIAIVVMVWYWRRYLGKWGALIAGLLLVISPYMLYYGRYVRNESFVAFSGIVMLYAML
                     RHFETGGKKYLLIMAGALALHFTSKETSFIYTAEALIYLAVYFIVQVTRRPWGEAERD
                     YRSFIISLGVAVLLAGAAVAFGLYSRDAATISATETAMPSDPALQATPPTEPETGAFS
                     PSLILAAAAIAALVAAGVFLIRGYTWDRIRNERSFEMLMVAGTIVLPMLTPFAIKGLE
                     RWLHVSIPTTAPEVQALSGDIKSVLTIGGLLILMFIISTVIGLLWNKEKWWKIALVFW
                     VPFTILYTTVFTNSDGFFTGTIGSLGYWIVQQDVQRGSQPWYYYLLIQIPVYEFLPAI
                     GFLLALVLGFRRKITTSIEEDVVDQNEEFVNLLKQEHEGEENTEEKNFSNTFSLLVWW
                     SIISLIAFTYAGERMPWLTVHITLPMILITGWALGHIIDTANWEKIQSQRVPLTLAAL
                     TVFIVSVMGMVLALNSPTPPFQGKELEQLQATSAFLLPTIMTLLSAVGLTYLFNGWTF
                     HEAKRAFVLLVFAFLAVLTGRAAFRSSYITYDQATEFLVYAHGATGIKEIMQQAEEIS
                     RRTTGGMGVALAYDASAPNTGVSWPFVWYLRDYTNQRSFDQPTRALRDSVVVIVDEKN
                     FDKIEPALGPGYYRVDYIRMWWPMQDYFNLVDERDPTQPFPEDYACSGALSFLKLVKS
                     KDYTTFCEGFTNPQIRAGIIQIWLNRDYTQYAEAKGRTNLTLATWDPAAKMRLYIRQD
                     VAAQIWNYGLAPSAVVPTEDPTEGKYIPLSSDLIFETFEKTPLNAPRSLAFAKDGSMY
                     VADSRNHRIVHISPLGKVINEWGTFADGVSVTVGDGTFNEPWGVAVGPDGSVYVTDTW
                     NHRIEKFSADGKFIKAWGFFGQGETPEAFYGPRGLAVDAQGRVYVTDTGNKRVVVFDA
                     DGNFITQFGSAGLDAGQFDEPVGITVDKNGTVYVVDTWNQRIQTFTAVETAGALTFLP
                     AKQWDVYGWFGQSVDNKPFIAVNNAFHVFIT"
     assembly_gap    11233..11332
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11402..12541
                     /gene="sucC"
                     /locus_tag="PLE39_14705"
     CDS             11402..12541
                     /gene="sucC"
                     /locus_tag="PLE39_14705"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNE69630.1"
                     /translation="MKLHEYQSKTIFSKYGIPIPKGRVAATAQEARQIAEELGGRVVI
                     KSQVLVGGRGKAGGVKLAKDSAEAEQLATQILGMEIKGLPVRKVLVDEASAIDQEIYF
                     AITDDRAAKKPVMIASAAGGVDIEEVAAKTPEKIIKVNIDPLLGLREYQARDVAVAID
                     LPREHWKDFVKIAMGLWEVYKNSDASLAEINPLVITKDKKLIALDGKMMIDDNALFRQ
                     PNLSELRDTDEDAPSEIEARKYGLSFIKLDGNIGCMVNGAGLAMTSMDVIKLFGGEPA
                     NFLDVGGGAGAEKVAAAMRIILTDPNVKAVLFNIFGGITRCDEVARGILAAMDEVKPK
                     VPMVVRLVGTNAEEGRRLLENANMITAETLADAAKKSVEAAKKGN"
     gene            12545..>13241
                     /gene="sucD"
                     /locus_tag="PLE39_14710"
     CDS             12545..>13241
                     /gene="sucD"
                     /locus_tag="PLE39_14710"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNE69631.1"
                     /translation="MSILVDKNTRLIVQGITGNEGLFHTTQMVAYGTNVVGGVTPGKG
                     GEWVLDGKLPVFDSVKTAKEATDANATIIFVPARFAPDAMFEAADAGIPLVICITEGV
                     AVQDMMRVRNYLDQKKVRLIGPNCPGLLTPGEAKIGIIPGNIAIPGNIGVVSRSGTLT
                     YEVLYAMKNLGMGATTCVGIGGDPVNGTNFLDVLEMFEHDPKTEKVIMIGEIGGSEEE
                     KAAEYIAKKMTKPV"
BASE COUNT         3444 a         3256 c         3215 g         3226 t
ORIGIN      
        1 cttaggaatc ccagctctgc cagattcata atatcctcgt cgtccaaggg ctcgccatat
       61 ctttgcgcca ccaaatcacg caggctgtgg ttgtcataac tgtcacccca tgcgccatat
      121 accccgccga tgccaagcgc agggttgtac accgtgctaa agccaaaagg tatacctgaa
      181 gctacaggaa tgatctcctt tttgatattt ggaagttcaa aaaatgatcg atgtttgttc
      241 atgtgattga taatctcctc tcttgcgcgt ctttaaagct tgtctctata cagcaaacaa
      301 cacccatgac gcagttaagt tacttgcatg ttgaagaaaa tttatttatt cattcgctgt
      361 tgttacctta aaatattttg aaaacttttc acaaactctt gtaattaatt ttattgttgc
      421 tatactaaac aacaagtaaa tggaatatca aaggagatat acaacatatg atgatgacaa
      481 caactacaac gatggaaacg cttgcaataa agactgaaaa taaattcagg acacttgtga
      541 ccaagcaatc ctataaaaaa ggcgaaatga tttgcgccat gccgagtgag aacatcacag
      601 atagaccgaa ccgtttcaca gtgcagatcg ggcgggaggt tcatacgcat gttgggaaac
      661 ttgcggcatt gaatcactca tgtgacccca atgttatttt ggacacgaag aagatggtca
      721 tggttgcccg ccgcgatatt gaaaaaggtg aagagctttc cttcttttac ccctctacag
      781 aatgggaaat gcaggcgcca ttcatttgca tttgcggatc agcgaactgc atccacgttg
      841 tagcgggcgc gcgcttcctg ccgctctcca cacttgaaaa tcattacctt aatccgcaca
      901 ttcgtgaaat gatgatcgaa ttacttaaca acacccagga acacctcaag agcttgaagt
      961 aattctcaga acaaaactga aacataaaag aaaaggacat cggctactgc gatgtccttt
     1021 tcttttataa aaaactataa ttttcggtgt aaagccagca gggcgtaggc attttcccag
     1081 ggcttgccaa gccggtggtt gtctgaaact tcgattttat tgagtttcat tgctatttgc
     1141 ggcaaaagcg atgcaatggt agcccagtca tcacccagca cggcagactt cattttatcc
     1201 gcaagctgcc ccgtccacct ccattccatg cggaatttat cggcttttac ccaatagtcc
     1261 cgcttctccc aggctgccac tgaggcatcc accccatctg agatggattg cagcgccagt
     1321 gcaataaatg aagccatatc cttcgcctca ggcgtcactt gagacatttt catcatttca
     1381 cggatgcata gcacgatgga ttttgtcaaa cgggttcgtt cttttcctac agaatcgggg
     1441 ttgattacac ggctcaaggc aatttctcca gttgtttttt gacacaagcg cgggattata
     1501 ccgctgattt cagggggatg gacaaaaaca aaatttgttg acgcaccctc tcccctgctt
     1561 tataattcgg cccgctcaat atttgagttg gggacgtgtt ggaattggca gacaagcatg
     1621 acttaggatc atgtgccgaa aggcatgtgg gttcgactcc caccgtcctc actattttac
     1681 aagacccgtc gtaagtgctt ttatccaggg tctggaggaa actttgaata tcgaaaaaca
     1741 aattcaggaa gaccattcag ccaagctcat cgttgatgtt gagcaggaaa aaatggatgt
     1801 ttacaaacgc cgtgcagcta ccaagatttc ggcgcgcagc aagattcccg ggtttcgccc
     1861 gggtaaggct ccatacgatc ttgttgtaag aacctttggt gaaggtgctg tcaccgagca
     1921 ggccattgac ctgtttattg atgccgaata tggaaatctt ctcaaggaag cggatgtaaa
     1981 tcctggtgca gctggttcgc ttgaatcgat cgacagcctc acgcccccca agttaacttt
     2041 ccgcgtaccg cttgctccgg aagtggacct cggcacctat catgatatcc gcatggaata
     2101 cgaatggcag gctcccgatc aggcggcagt tgatgccgct ttggaagacc tgcgccagat
     2161 gtatgcatcc actgaaacgg ttgaccgcgc cattgaactc ggcgattatg tgcttgtcga
     2221 tgtgaattcg gaaaccgctg aagtaacccg caagggattt gccacctttg tccgcaagga
     2281 agaccgcgac accgaatggc cctacaacgg ttttgcaaaa gagctcattg ggctgaaggc
     2341 tggggagagc aagaccatcc aacacacctt ccctgctgat tgggaattgg aagagcttcg
     2401 cgacaaggaa gtgacgatgg aagtcacggt caagactgtg cgcggtgtaa ccctgcccga
     2461 tatcgatgat gcgtttgcaa agatgaccgg cgctggcgaa accgtagatg agttgaagga
     2521 agctgttaag aaggacgtgg aagcccgttc acaggccgag tatgatgaca agtacttcgt
     2581 agagttgatc gaaaagatca aagaagttgc caccattaaa taccacgaac acaccctcga
     2641 acacgaaggg gagcatgttc tttcagatct gagcaatcgc ctggctcaac agaacatgga
     2701 tcttgatacc tacttcaaga tgcgcggaac cacccgtgaa caattcattc aagatgaagt
     2761 gaaccccgtg gcaaagaagc gccttgagcg cggattgatc cttgatgaga tcgttcgtca
     2821 ggaaaagatt cagatcgaca acgaggctct cgaagctgaa tacaacgtta cgctcaatgg
     2881 tcttgcccaa caaggcatgg actttagcaa gattcgcggc ggcagaaaag gccaaaagca
     2941 gttgagcgaa gccattgcaa tggaatccgc cagccgtgtg atgacccgca aagcattgga
     3001 tatgatcaag tccattgcca ccggggagta taagccgatt gaagagagac aggctgaggc
     3061 gagtcctgaa gcgggcgaag cttctgaaac agagtcaaaa cctgagtaaa cctttcaagg
     3121 gcgggttaat aatccgccct tgaaatttta ataaaaatcc aatacacgct gtgtatgatt
     3181 gtcccaatca aaaaggagac aaaaatgatt ccagacttta agaatgtagt tccaatggtt
     3241 gttgaaaata ccggccgcgg cgagcgcgct tatgacattt actcgctttt gttgaaggag
     3301 cgcattgtct tcgtgggaac acccattgac gaccaggttg ccaatgtgat cgtggcccag
     3361 ttgcttttct tgaaccatga agatcctgaa aaggaaattc gcatgtacat caactcgcct
     3421 ggtggtcaga tctatgcggg tcttgccatt tacgacacga tgcagatcat ctcgaacccg
     3481 atcagcactg tggctgtagg cgtgaccgct tctttcggga ccgtactgct gaccgctggt
     3541 acgaagggcc gccgttacgc tctgccccat gccaccgtcc acatgcatca gcctcttggc
     3601 ggtgcgcaag gacaggccac cgatattgag attcaggcca aacagatcct gcgcttgaag
     3661 gctttgttga atggtgttct cagcagccac actggccagc cgcttgaagt gatcgaacgc
     3721 gatgcagacc gcgattttta ccttgatgcc cagcaggctg tggattacgg cttgatcgat
     3781 caggtcattg agatgccagt gaagagcaaa taaaaaaata agcacgtaaa cattaaaagg
     3841 cctcgaggag aaatcttcga ggccttgcgt ttataatatc gtcacaaccc actatttttg
     3901 aagtttagac tcgctaaaca ctttaaatca aaaatcagaa actggaaatt gccatgactt
     3961 tttcaattgt tgcatgtgat ctgaaagaaa agacctgggg gattgccgta gcctcaaaat
     4021 ttcctgcggt aggcgctgtg gttccatggg cggcggctgg tgctggagcg gttgccactc
     4081 aatcgtttgc aaatacttcc ttcggcccac gcggactgga aatgatggct gcaggccttt
     4141 cagcggatga aacactggct aaactgctga tggatgaccc cgatcgtgaa ttgcgccagg
     4201 tcggtctggt ggatgcaaac ggcaaatctg caacctacac aggcagtgga tgttttccgt
     4261 gggcaggcgg ggtcaacggg aagggctatg ccattcaagg aaatatcctt gcgaatgcga
     4321 aggtggtgcc tgcgatggaa aagaagttcc tcgctacaaa aggtgacttg ccgacacgcc
     4381 tccatgccgc gcttttggct ggtgaccgcg ccggtggtga caaacgcgga cggcagtctg
     4441 ctgccattta tgtagtgaag ccgaatggcg gctacggcgg ctacgtagac cgttggattg
     4501 attaccgcgt agatgaccac gaagatcctg ttccacgttt gggcgaattg cttgaactgc
     4561 accgtctgta ttttgataaa agccctgaaa acgagcgtgc cgccttgaaa ggcaaaactc
     4621 tggagcaaat gacgtccatt ttgaaacgtg cagggtatct attaaagaaa aacaccgctt
     4681 ttgtagatgc attcaacgct tttatcggaa acgaaaactt cgaggaacgt gccgacccga
     4741 acggccaatg gattgacaag cctgtgctga aatatctagt gaagaagttt gggaagtaac
     4801 cagcgtatgg cgctggaaaa tcgcaaatca aaatcataaa ataaaaaaac actcatgatc
     4861 ccttcaaata taaaagctct cattcttgat atggatggcg tcatctggaa agccgatgcg
     4921 cccatcggcg acttgccatc cacgttcaac cgcatccgcg agcgcggatt gaaattcgtc
     4981 ttcgcaacca ataacggcac aaaaacacct gaggattatc agcaaaaact ggccgacctg
     5041 ggtgtggata tagccccctc acaagtagtg acttcagcca tgggcatcgc gttcatgctt
     5101 gcccagagat ttccacgtgg gacgaagatc tttatgatcg gcgaagatgg catccgcgtg
     5161 gctttggagg aaaaaggatt cgagatcctc agcgcagaaa atgcttcaca ggcgcaagcc
     5221 tttgtgatgg gcatagaccg tggaatcaac ttccaaaaag ttgcggaagc aactctgctt
     5281 gtccgcgctg gaattccctt ttacaccacc aataccgaca agacctttcc cactccacgt
     5341 ggagaaattc cagggtcagg cgcttgggtc tcggtcattc aaacagccac gaacgtagaa
     5401 ccgatcatcg cgggcaaacc gtttccattc ctgatggaac tctctctcga aaaacttggt
     5461 acgaaaaagg aagagaccct cgttgtcggt gacaggcttg aaacggatat cgccgctggt
     5521 caaagtgtag gctgccccac tgcgttggta ttgagtggag tttcaacaaa ggcacaagct
     5581 gacgcgtgga cccccaaaat ggatatcatc gcagcaagcc tggccgactt ggtgaagtaa
     5641 aagaatatgc ttgtctgaag gcgaattaca taatcaagat tctaaaagaa cggcatctca
     5701 ggatacttcc tgagatgccg ttcttttttt ccaactacta tcttgttgct taattcgggt
     5761 acccacccgg agtctttgtt ggtgtaggca ctgtaccgat cttgataacc accgtcagag
     5821 aatcgccgaa ggcgaccgac ttatcgtttg aaaggcgcca ggtgcctttg atctcaccag
     5881 attttgctgg agcggtcaag ttcacagaga tgtccacgct ttcaccaggc tttacttcct
     5941 tgccgatcgg ggtggattgt ccattcatgc catcccctgc aacaaaaaca agtttgtatg
     6001 tcgcggtcca ggtacaggca ccggtattgg tcactttcca ggttttggtg aaggtttggc
     6061 ctgctgtcat tgcagtccca tctggtacgg tcacatcaga aacataaatg gacatgtcgc
     6121 atccactggt agggacagct tttgtggccg tggcaaggat cgtcaacggt tgggtggtca
     6181 cagttgccaa cggagcaacc gtgggggaag gaacaagcaa ggctgtattc agggttgccg
     6241 ttggggttac agaaagcgcg atcgcggtct gccctgcatt cactgtagct gctgcattgg
     6301 tgtagatcac attgatgtca ccctcggaag cctgttcact ttttccgcct ctgaaggcgt
     6361 acaccagtgt acagatccca gccagaacca gaacagccac aagtccccac agaattgctg
     6421 gcctagattt caaagcattt atatcgaaat ttttcaaatt cattcaattc tcctttttga
     6481 caaggcacaa attatagtgt gggtaaatta aaagaaatta atcgtttgac ctgcgaattc
     6541 cctacttttc acatactcaa cgagcatact gaatatttcg gcctaaaaga tgcaaaaaga
     6601 atggcatcaa ccatacggta gagtaaaatc aggaaatgct aatttatgat ttcgaccaaa
     6661 aaacacttct agacctcctc acccgctggg gtgaacctgc ttatcgggcc aaacaaatct
     6721 ggcagggact ataccaacat ctttatagtt cacctgagca attcaccaat ctgtccaagc
     6781 cgttgcgcga taaactcgcc gaagggttga actttgcgcc gcttaagccg aagctttacc
     6841 tcgattcgtc cgatggacaa acacgcaaga ctcttttcga gttaggtgat ggtcaagtga
     6901 tcgaatccgt tttgatgcgc tacggtgacc ctgccgacaa ccacgacacc agcggaagag
     6961 atatccgctc acgaaggacg ttgtgtattt ccacacaggc agggtgtgcc atgggatgcg
     7021 tcttttgtgc cacaggtcaa atggggttca aacgccacct aagcagcggt gaaatcgtgg
     7081 cccaggtgat gtactatgcc caaatgctca aagaggagaa cctttcggtc acgaacatcg
     7141 tctttatggg catgggtgaa ccgttccaca actacgaaaa tatgatggcc gccatcgacc
     7201 gcctcaacga tgcggacgga ttcaactttg gcgcgcgtcg aatgaccatt tccacggttg
     7261 gtctggcccc gcaaatcaaa cgctttgccg acgaacacag acaggtgaat cttgcgattt
     7321 cccttcatgc cgccgatgat gatgagcggc ttgccattat gccagtcaac aagcggtata
     7381 agatcgccga tgtaatggaa gcctgcaaat attatgtgga aaaaacgcat cgccgcgtga
     7441 cctttgagtg ggctctgatc aacggagtca acgacacccc ggaaacagcc aaaaaactcg
     7501 ccgccagact tagaggtctg ttatgccatg tcaacgccat tcccctcaac cccactactg
     7561 gatatgaggg cgaagcgacc tcacgtgaaa gggcgaacct gttcaaagaa tcgctggagc
     7621 aggctggggt tccgtgtacg attcgtatgc gccgcggcat tgatattgcg gcaggttgcg
     7681 gtcagcttgc tggttctgtt aattagaaaa atttctattg ctgattacga gagtaataaa
     7741 agtagggatg ttattttggc tacacgaaat ttcaaagaat taacttccgc tttaacttaa
     7801 acgcatatca tttcttgtca gaacaatttg attgatgtat agtgatgcaa cttttgactt
     7861 aataaggtga cgctcaatgg aacaaaaaca aaactggctc gaacgcccca ttcatcctgc
     7921 attaccaggc cttacaaatg agatcgcgat ttttattggc gtgattctgc ttgccataat
     7981 cacaaggttt tacgaccttg gcacacgcgt aatgagtcac gatgaaagcc tgcacaccta
     8041 tttctcatgg ttactctatc gcgggcaggg ctacgaacat tcacccatga tgcatgggcc
     8101 gtttcaattt cacattgtcg cattaactta ttttctgttc ggtgcgagtg acttcacttc
     8161 ccgtattcct gcggcattat tcagcatagc cattgtagta atggtctggt attggcggcg
     8221 atatcttggt aaatggggtg cattgattgc aggtcttttg ctggtcattt ccccttacat
     8281 gttgtattac gggcgctacg tccgcaacga gtcgtttgta gcattctcgg gaattgtgat
     8341 gctatatgcc atgttgcgcc attttgaaac aggcgggaaa aaatatttac tgatcatggc
     8401 aggcgccctt gccttgcatt tcacatcgaa agaaacttcg ttcatctaca cggctgaagc
     8461 gctgatttat ctggccgttt attttattgt ccaggtaaca cgccgcccat ggggcgaagc
     8521 ggagcgcgat taccgctcct ttatcatttc gcttggggtt gctgttttac tggctggtgc
     8581 tgctgttgca tttggattgt actcacgtga cgcagccacc atctctgcga cagagacggc
     8641 catgccaagc gaccctgccc tgcaggcaac gcctccaacc gaacctgaaa ctggtgcttt
     8701 ttccccaagc ttgattctcg ccgcagcagc catcgctgca cttgtagcgg ctggtgtttt
     8761 ccttattcgc ggttacacat gggatcgtat tcgtaacgaa cggtcttttg aaatgctcat
     8821 ggtggcaggg acgatcgttc tccccatgct cactccattt gcgatcaaag ggctggagcg
     8881 ctggcttcac gtcagcatcc ctacaactgc ccctgaagtg caggctttaa gtggagacat
     8941 aaagtcagta ctgaccatcg gcgggctttt gatcttgatg tttatcatat ccactgtcat
     9001 tggcttgctg tggaacaagg aaaaatggtg gaagatcgcc ctcgtatttt gggttccatt
     9061 caccatactt tacaccaccg tttttaccaa cagcgacggc ttctttacag gcaccattgg
     9121 ctcgctcggc tattggattg tccagcagga tgtccaacgt ggcagccaac cctggtatta
     9181 ctaccttctg attcaaatcc cagtgtatga atttctacct gcaatcggct tcctgcttgc
     9241 tcttgtgctt ggcttccgca gaaagatcac gaccagcatc gaagaagatg tggtcgatca
     9301 gaacgaggaa tttgtcaacc tgctaaaaca agagcacgaa ggagaagaaa acacagagga
     9361 aaaaaacttt tcaaacacat tcagcctgct tgtttggtgg agcattatca gtttgatcgc
     9421 ttttacctat gcaggcgaaa gaatgccctg gctcaccgtc cacatcacac tgccgatgat
     9481 tcttattaca ggctgggcgt tgggccacat cattgacacc gcaaattggg aaaagatcca
     9541 aagccaacgc gttccattaa ctctcgccgc actgaccgtc tttatcgtca gcgtcatggg
     9601 catggtgctt gctctgaaca gccccactcc cccattccaa ggcaaggagt tggagcaatt
     9661 gcaagccacc agcgctttcc tgctgccaac tattatgaca ctactcagcg ctgtgggtct
     9721 cacttacttg tttaatggct ggaccttcca tgaagcgaag cgagcttttg tgttgcttgt
     9781 atttgcattc cttgcagtac tgactggccg tgcagcattt cgatcttctt atatcactta
     9841 cgaccaggca acagaatttc ttgtctacgc gcatggagct actggaataa aagagatcat
     9901 gcaacaagcg gaggaaattt ccagacgcac cacaggcggc atgggagtag cccttgccta
     9961 cgacgccagt gctcccaaca ccggtgtttc atggcctttc gtttggtatc tccgtgatta
    10021 cactaatcaa cgctcgttcg accagccaac ccgcgccttg cgcgactcag ttgtggttat
    10081 cgtcgatgaa aagaatttcg ataagatcga gccagctctc ggcccgggtt attaccgcgt
    10141 ggattacatc cgcatgtggt ggccgatgca agactatttc aatctggtgg atgagcgtga
    10201 tcccactcaa ccattccctg aagattacgc gtgcagcgga gcgttgagct ttctaaaact
    10261 tgttaaatca aaagattaca caacattttg tgaagggttc accaacccac agatccgtgc
    10321 aggtatcatt cagatctggc ttaatcgtga ctacactcaa tatgccgaag ccaaaggccg
    10381 tacaaacctg actctggcca cctgggaccc cgctgcaaaa atgcgccttt acattcggca
    10441 ggacgtcgcc gctcagatat ggaactatgg cctggcgcct tctgcagttg tacctactga
    10501 agatccgact gaaggaaaat atattccgct ttcatcagat ctgatctttg aaacctttga
    10561 aaagacacct ttgaatgcgc ctcgttcgct ggcttttgcc aaggatggct cgatgtatgt
    10621 ggccgactcg cgaaaccatc gtatcgtcca tatcagtcca ttgggcaagg ttattaatga
    10681 atggggaacc ttcgccgatg gtgtcagcgt tacagttggt gatggaacat tcaacgaacc
    10741 ctggggtgtt gccgttgggc ctgacggctc ggtatacgtt acggacacat ggaaccatcg
    10801 cattgaaaaa ttcagcgcgg acgggaagtt tattaaagct tggggcttct ttggtcaggg
    10861 agaaacaccc gaagctttct acggcccgcg cggattggcg gtggatgctc aagggcgagt
    10921 ctatgtcact gataccggga acaagcgtgt ggtcgttttt gatgcggatg gtaatttcat
    10981 cactcaattt ggttcagcag gtctggatgc aggtcaattt gatgaaccag tgggcatcac
    11041 agtggataag aatggaacgg tctatgtcgt tgatacatgg aatcaacgca tccaaacctt
    11101 caccgctgtg gaaaccgcag gcgcacttac attcctccca gccaagcaat gggacgtata
    11161 cggctggttt ggtcagtctg tagacaataa accctttatt gcagtgaaca atgcatttca
    11221 tgtcttcatc acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcttgcga
    11341 ggattttttt ctggtgtata atcgcgatgc tttataaaaa aacatccccc gagggagaat
    11401 aatgaaactc cacgaatacc aatcaaagac aattttttca aagtatggca tcccgattcc
    11461 aaagggaagg gttgcggcta ccgctcagga agcaagacag attgctgaag agttgggtgg
    11521 ccgcgttgtc attaaatcac aagtactggt cggcggacgc ggtaaggcgg gcggcgtaaa
    11581 gcttgccaaa gactctgccg aggcggaaca actcgccact cagattttag gcatggagat
    11641 caaaggcctg cctgttcgca aagtccttgt tgatgaagcc tctgccattg atcaggaaat
    11701 ttattttgcc atcaccgacg accgggcagc caagaaaccc gttatgattg cttctgcagc
    11761 aggcggggtt gacattgaag aagtagctgc caagactcct gaaaagatca tcaaggtcaa
    11821 tattgacccg ctgttgggat tgcgcgaata tcaagcccgt gacgtagccg tcgccattga
    11881 cctgccgcgc gaacactgga aggatttcgt caagatcgcc atggggttgt gggaagttta
    11941 caagaactca gatgcatctc tggctgagat caacccactt gtgatcacca aagacaaaaa
    12001 gctgattgcg cttgacggaa agatgatgat cgacgacaac gcccttttcc gccagcccaa
    12061 tctgagcgaa ctgcgcgaca cagatgaaga tgccccttcc gagatcgaag ctcgcaaata
    12121 cggcctctcc ttcatcaaac ttgacggcaa cattggatgc atggtcaacg gcgccggcct
    12181 ggccatgacc agcatggatg tgatcaaact ttttggcggc gaaccagcca acttccttga
    12241 tgtaggcggc ggtgcaggtg ctgaaaaagt ggcagctgcc atgcgcatca tcctcaccga
    12301 cccgaatgtg aaagccgtac tcttcaatat cttcggcggc atcacccgct gtgatgaagt
    12361 tgcccgcggt attctcgctg caatggacga agtcaaacca aaagttccaa tggttgtccg
    12421 ccttgttggc acgaacgccg aagaaggtcg tcgcctgctt gaaaatgcaa atatgatcac
    12481 cgcagaaacc ctcgccgacg cggcgaagaa atctgttgaa gccgcgaaga aagggaatta
    12541 atccatgagc attttagtag acaagaacac acgcctgatc gtgcaaggga tcacaggcaa
    12601 cgaaggttta ttccacacaa ctcagatggt tgcctatggc accaatgtgg tgggcggcgt
    12661 cacccctggt aaaggcggtg aatgggtgct cgacggaaaa ctccccgttt tcgattcggt
    12721 caaaactgcc aaagaagcca ccgacgccaa tgccaccatc atctttgtac ccgcccgttt
    12781 cgccccagat gccatgtttg aggccgcaga tgctggcatc ccccttgtca tctgcatcac
    12841 tgaaggtgta gccgttcagg acatgatgcg tgttcgcaat taccttgacc agaaaaaggt
    12901 ccgcttgatc ggccccaact gccctggctt attaactccc ggtgaagcca aaatcggcat
    12961 catccccggc aatatcgcca tccccggcaa cattggcgtg gtttcccgct ctggcaccct
    13021 cacctacgaa gtgttgtatg ccatgaagaa ccttggcatg ggcgccacca cctgcgttgg
    13081 tatcggtggt gaccctgtca acggcaccaa cttcctcgat gtgcttgaaa tgttcgaaca
    13141 tgaccccaag accgaaaagg tcatcatgat cggcgaaatt ggcggtagcg aagaagaaaa
    13201 agccgcagaa tacatcgcaa agaagatgac caaacccgtt g
//
LOCUS       DAOQJC010000110        13124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2315_length_13124_cov_10.331318, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000110 DAOQJC010000000
VERSION     DAOQJC010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13124
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2315_length_13124_cov_10.331318"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..3036
                     /locus_tag="PLE39_14715"
     CDS             310..3036
                     /locus_tag="PLE39_14715"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNE69632.1"
                     /translation="MISPILATKLYSPPPRPGTVTRLRLIERLDEGLRSGHKLSLISA
                     SAGFGKTTLVGEWITKSGLPVAWLSLDDGDNDPVRFISYLIAALQTIKAEIGESLLAA
                     IQAHQPPSNESILTALLNEITVHLKEFLFVLDDYHTIDSKPVDEILLFLIEHQPPQMH
                     LVITSREDPALPLARLRVRGQLTELRAADLRFTPTEAAEFLDHAMNLNLTTDEVTVLE
                     SRTEGWIAGLQLAALSMKGQEDVHGFIQAFAGDHRYIVDYLVEEVLQHQPEQIRDFLL
                     QTSILERLNGPLCDVLTAQSGSKLKLEQLQRGNLFLIPLDDKREWYRYHHLFADVLRM
                     YLTTEQPDLVSVLHLRASEWYEQNNLTAEAIRHAVAGENFERAAGLIEKTLPLMRQSR
                     QESAMLGWFKALPEELLRKHPVLNVNYAGTLLQVGQFDGVEARLQDVEQWLATPEDIR
                     ERPVYVSEEEFQRLPSSVAMYHAAIALAQGDVADSIKYANKVLELAQTDKDFPRGAAS
                     SLLGLASWTGGDLETAYRLFADGMDHLLKVGFISDVVGGSITLADIRITQGRLHEAMS
                     IYERGLQLATKHEVPILRGAADMYVGMSELHREWNDLLAAEQYLLKSRELGELNGLPK
                     NPYRRRFAMARIHESRGNFEEALELLNEAEPLYIGDFSPNVRPIQALKVRVWIKQGEL
                     EKALAWARERELSSTESPSYLREFEQITFARILLLQYESDAADSLLHGVTQLLENLLK
                     AAESGGRMGSVIEILVLQALAYQMQADYASALKSLERALKLAESEGYIRIFVDEGAGM
                     AGLLRKALAKGIMPDYIGKLLTAFKVADQGLGVEMSPSAVPVSSSMIEALSQRELDIL
                     RLFKTELSGPEIAQELVIALSTVRTHTKSIYNKLNVNSRRGAVKRAIELGLI"
     gene            3209..3940
                     /locus_tag="PLE39_14720"
     CDS             3209..3940
                     /locus_tag="PLE39_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69633.1"
                     /translation="MKTNGKTSIKESKMKMTTSTLMRLAGVSAVLAGLCFIIIGMFHP
                     ENVTSSVTTTTWVNVHIAATALGFFGLFGLAGLYARQVEKSGWLGLTGFLLFSIWFVL
                     VSGFSFVEAFILPQLAAESPAFVESLLGMFTNTPTTIDLGVLPTLWNVSGPMYILGAL
                     LFGIATFRAQVLPRWAAGLLTLGAVLIPIGGMVPHEYQAKIAMIPIGLSMAWLGSALI
                     FERRMKASESLISQRISTPEASKVA"
     gene            4015..4704
                     /locus_tag="PLE39_14725"
     CDS             4015..4704
                     /locus_tag="PLE39_14725"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HNE69634.1"
                     /translation="MKAMIQENTQIKITARKAQTSWWIPAGLILLSVLPLTFGAIRLS
                     QLINGAEITPANARFFAAPLPVVIHIISSAIYAVLGAFQFVSRLWQRGIKWHRWVGRL
                     LVPFGLFVGFSGLWMTLFYPRPTNASDLLYALRLFFGSGMILSIIIGFLSIRRRNVAE
                     HQAWMTRAYAIGMGAGTQVLTGIVGALVIGEVNEFENALLMGAAWVINLAIAEWNIRR
                     DATRQIRTTQV"
     gene            4752..5672
                     /locus_tag="PLE39_14730"
     CDS             4752..5672
                     /locus_tag="PLE39_14730"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNE69635.1"
                     /translation="MKAIVANQYGGPEVLQLKEVGKPQPKDNEVLIKVHATTVTMGDF
                     RMRSFTVPPVFWLPARLTLGITKPKQPIYGMELAGRVESIGKNVTRFKIGDQVFASTL
                     TENFGAYAEYKCLPEKAMMAIKPANISFEEAAALPIGATTALRLLRKGKIQRGQKVMI
                     YGASGSVGTYAIQLAKHLGAEVTGVCSTANLEMVKSLGADHVIDYTKETLSATKERYD
                     VIFDTVGKFAKSKASQILMPNGAFITMAQLDSKESMDNLIFIKELVEAGKLKVVIDRC
                     YPLEDMVEAHRYVDSGRKKGNVVITVGNTK"
     assembly_gap    5743..5842
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5843..6263
                     /locus_tag="PLE39_14735"
     CDS             <5843..6263
                     /locus_tag="PLE39_14735"
                     /inference="COORDINATES: protein motif:HMM:NF022606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2938 family protein"
                     /protein_id="HNE69636.1"
                     /translation="FKIPSSNFCIVGRWILYMPEGVFRHSNINAAPKKSAECITGWAT
                     HYLIGTIFAFIFLAVTGNDWLQTPRIFPALIFGTVTVSAPFFIMQPAFGQGIAASRAG
                     NPAQARFRSLINHLVFGFGLYLFGLMASWMTWKWTFA"
     gene            6361..6885
                     /gene="tpx"
                     /locus_tag="PLE39_14740"
     CDS             6361..6885
                     /gene="tpx"
                     /locus_tag="PLE39_14740"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNE69637.1"
                     /translation="MTTVRNDVLKIGDKFATLLGDEVSVGQAAPEFTSVVPGWAPVNP
                     IQESKGKVIILSAVPSLDTDVCDRETRRFNEEASKLGDDIIIYTISTDFPMAQKRWCG
                     AAGVDKVKVVSDVLETEFGLKYGLVIKERRFLRRSVFIVGRDGKLTYVKYLPTLGEEP
                     NYDEVINAAKEALK"
     gene            complement(6982..8259)
                     /locus_tag="PLE39_14745"
     CDS             complement(6982..8259)
                     /locus_tag="PLE39_14745"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNE69638.1"
                     /translation="MSELAILGGPKTRTEAYPQWPVWDQRDIDAVVDVVKSGRWGGYP
                     YPGPKTAELAKKFAELQGGGYAVPMINGTVTMEVALRAAGIGWGDEVIVPAYTFQATA
                     SAPMGAGAIPVIVDVDPNTYCLDAKAAAKAITPKTKAIIPVHLGSNMADMDAIMELAE
                     KHNLIVIEDCAHAHGAKWRGKGAGTIGHFGSFSLQSSKTLTAGEGGILLCRTPEFAAL
                     AASVIDCGRPHALGGGGEDKNGLAMQGGNFRMSELQAAMALVGIERFPAQAKQREEMA
                     AYMDEALSEIKGVRVMKRDERHTTRSFYRYIFAIDPQEFGVEHDVLCGALDAEGVDCW
                     VGYEAMHNYELFQPQRSKLAVPNAFPEYFDFKNMNLPEATRACEHEAVWLDEAIFREG
                     PKGVDDVIAALKKIQKNADELNSAADELRKKYG"
     gene            complement(8276..9454)
                     /locus_tag="PLE39_14750"
     CDS             complement(8276..9454)
                     /locus_tag="PLE39_14750"
                     /inference="COORDINATES: protein motif:HMM:NF013473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE69639.1"
                     /translation="MQPTTRSLFLMRVYYLLWTGAGGFIFPFISLFYAQKGLSGTQMG
                     WLTTIGSLIGLVSAPFIGRISDNAANPRRVLQLCLFGSGVLMLILGQQSLFIIMAVII
                     AVEAFVGAPVFPLSDAQALSISSEKEGFGSIRSWGSLGWAITAFIGGWMVSRAGLVSI
                     FWGYAALYGLCIFILGRIITTPKPASASEEHHLPLGKVARSLFTQHTLLGLTIALSIF
                     WLANGGRHQFETLYMHQLGASEQLIGWAYTYPALMELPIMLWADKLVRKYGSGKILSL
                     SILIEGISLFIIVIHPSIATIMFMRAASGFYYSFYAIASVGYAVENAPEGQGATILSL
                     YYVTLNGILALFNAPLSGAIFDWVGAYPLYIIAAAGTILAWLALVISQRKPAIQLQAS
                     "
     gene            complement(9507..10844)
                     /locus_tag="PLE39_14755"
     CDS             complement(9507..10844)
                     /locus_tag="PLE39_14755"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69640.1"
                     /translation="MPKIVIIGGGSYTWGPTFMRDLFATPELAGSTLVLHDILQDRVD
                     LVYALGQKMLHDFGLDFQLEKTLSLEEALQGADFVILTITTGRLESMRPDLEIPAKYG
                     IKQSVGDTTGPGGLARALRNIPVVAEIGRKVMEICPNALFLNYTNPMTVLTRTLAMQG
                     VKVVGLCHEWIGVREKLALIFGTSPEKIQAQIAGVNHLIWVTDLYADGKRVWDELPAI
                     TERILSGEIKVDEEDTSVFTDHAKVKSTLFQLYGALPAAGDRHIAEFFPHFINELTHW
                     GADYDLKLTNIADREALEAFAKTMIESVLNGETDLKPFMEDISTEAANKIIRAVTSGE
                     NYIGIMNLPNVGQVTNLPNIAVVETYGVISNTGAHAIAYGEVPAGVQSVLQKHISNQE
                     LTVQSALTGDWDLALQVLLNDPLSSRLTIPQAKQLLRELLEANKQYLPFFFGE"
     gene            complement(10895..12079)
                     /locus_tag="PLE39_14760"
     CDS             complement(10895..12079)
                     /locus_tag="PLE39_14760"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNE69641.1"
                     /translation="MNIGYAQQIITPTLDKPVYLAGFGQNRRAETVHDDLYVRALTIQ
                     TEKITLALVALDLIGFFRHDVLEVVNALKSSAADLQIVIASTHTHHGPDTMGLWGPDD
                     KTCGADQGYMSAIKKKIVDTVIASLSDLKPASAKWTSLHVPGLAKNARNPEILDDELT
                     LLQFSTADDKPLATLFNFPCHPEVLWEHNPNITADYVGYLREEVEKQTGAPCIFFAGA
                     LGGMMTPDVKDHSFEEAEFMGRKLGEEGVKALRAVDSGPLTSVSFEKRIIKAKLTNIL
                     YKLAFGRKLLPDVRDKKGFITSEVNLIKIGGLWLATVPGELLPKLGLQLKAWMKEAGA
                     HVTGVIGLANDEVGYILPVEDFKYPWNPFKPGKHYEETNSIGKDISLKVMNSLKELVS
                     QK"
     gene            complement(12101..13072)
                     /locus_tag="PLE39_14765"
     CDS             complement(12101..13072)
                     /locus_tag="PLE39_14765"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNE69642.1"
                     /translation="MKYFLGIDVGSSKTHALIVDETGQCVGFGKSWGGNHQGVGYDGL
                     LNVLQESFEGAKEMSGVGNDQITGAGFGVAGYDFPSDRESHLQAISKLGLSCPVEIVN
                     DGTNGLLAGATRGIGVNVTAGSSNNCRGRNAHGKEGRIVGNGPTFGEYGGGIEIAYRA
                     LQAVNYAWIKRNPPTALTKVLLEATGAKDEADLMEGVSNSQYHIFPFIAIEVINAARN
                     GDAAAQEIVRWAGEELGWLAVSVARQIEMENDEVEIVQSGSIFEAGEIIIKPMRDVVL
                     KHCPRAKLIRLDGPPVVGAVILGMEQAGFDGYAVHDVIVRTAKEIVK"
BASE COUNT         3280 a         3376 c         3147 g         3221 t
ORIGIN      
        1 cagcaacatc tgccaccacg ccaaactgca ccgccgccgc tcgaattttg gttttaccag
       61 ccaaagactg cctccaaggc taaatgttat aacattataa caatatactc caacagggtg
      121 ttttttgtca acaatcggaa tgaaaattgt ttgtccaagc ttgcataaaa cttacccaac
      181 ttaacgcagg tataaattca agacctacgt acggaacttt ttggagattt aaaagaccca
      241 acatattgaa gcctgtctgt atggctacaa accatcatgt tttatttatg cttgttatat
      301 aatgaattta tgatctcacc aattttggcg acaaaactat acagtccccc accacgcccc
      361 ggtactgtta cacgcctgcg cctgattgaa cgactggatg aagggctccg ttcgggtcac
      421 aagttaagtc ttatttctgc ttccgctggg ttcggcaaaa cgacattggt cggtgaatgg
      481 atcaccaaga gcggcctacc ggttgcatgg ctatcgttgg atgatgggga taatgacccg
      541 gtccgtttca tttcctatct gatagcggcc ttacaaacca tcaaggcgga aataggcgaa
      601 agtttattgg cagcgatcca agcccatcaa ccgccttcca atgaatctat tttgacagcg
      661 ttgctcaatg aaatcactgt ccatttgaaa gaattccttt ttgttttgga tgactaccac
      721 acgatcgatt ccaagccggt tgatgaaatc cttttatttc tgatcgaaca ccagccaccg
      781 cagatgcatt tggtgattac ttcgcgcgaa gaccctgccc tgcccctggc tcgcctgcgt
      841 gtccgtggac agctcacaga attacgcgcc gcggatttgc gcttcactcc aaccgaagca
      901 gctgaatttc tcgatcatgc aatgaacctc aacctcacca cagatgaagt tactgtattg
      961 gagtcccgta ccgaaggttg gatcgctggt ctgcaactcg ctgcactttc aatgaaaggc
     1021 caggaagacg tccatggatt tattcaagca ttcgccggag accaccgcta cattgtggat
     1081 tatctggtcg aagaggtttt acaacaccag cccgagcaaa ttcgggattt tttgttacaa
     1141 acatccatcc tcgaacgctt gaacggcccg ctttgtgacg tcctcactgc ccaatcggga
     1201 agcaaattga aactggaaca attgcagcga ggcaatttgt ttcttattcc attggatgac
     1261 aagcgtgaat ggtatcgcta tcaccacctt tttgctgacg tacttcggat gtatttgaca
     1321 acagaacagc ctgatctggt ttctgtcttg caccttcgtg caagtgaatg gtatgagcaa
     1381 aataatttga cggcagaagc gatccgtcat gcagtggctg gagagaattt tgagcgggct
     1441 gcggggctga tcgaaaagac gctgccgctc atgcgccaaa gcagacagga atccgcgatg
     1501 ctgggttggt ttaaggcact tcctgaagaa ttgctacgga aacatcccgt gctgaacgtt
     1561 aactatgcgg gtacattgtt gcaggttggt caatttgatg gtgttgaggc acgcttgcaa
     1621 gatgtggaac agtggctggc taccccggaa gatattcgag aacggcctgt gtatgtaagt
     1681 gaagaggagt ttcaacgtct gcctagttct gtcgccatgt accatgccgc cattgccctg
     1741 gctcaaggtg atgtggcaga cagcataaaa tacgcaaaca aagtgcttga actcgcccaa
     1801 acggataagg atttcccccg cggagccgca tcatcattgc tgggacttgc atcgtggaca
     1861 ggtggcgatc ttgaaaccgc ttaccggtta ttcgccgatg gcatggacca tttactgaag
     1921 gttgggttca tctccgacgt agtcggcggt tccatcactc tggccgatat acggatcaca
     1981 cagggtcgtt tgcatgaggc gatgagcatc tacgagcggg gattacagct tgcaacaaaa
     2041 catgaagtgc ccattcttcg cggtgccgca gacatgtacg tggggatgag cgaactccat
     2101 cgtgagtgga acgatttgct cgccgccgaa caatatcttt tgaaaagcag ggaactgggc
     2161 gaacttaatg gattgcccaa aaatccttat cgcaggcgtt ttgccatggc acgaatccat
     2221 gaaagccgcg gcaattttga agaggcactg gaactactca atgaagcaga acctctgtat
     2281 ataggtgatt tctccccaaa tgttcggccc attcaggcat tgaaggtgcg agtttggatc
     2341 aagcagggcg agttggaaaa ggctcttgcc tgggcgcgcg aacgggagtt atccagtacg
     2401 gaatcgccca gttacctgag ggaatttgag caaatcactt ttgcaagaat tttgcttttg
     2461 caatatgaaa gcgatgctgc agatagtttg ctgcatggtg taacacaatt actggagaat
     2521 cttctgaagg cagcggaatc aggtggcagg atgggaagtg tgatcgagat tctggtcttg
     2581 caggcattgg cttatcaaat gcaagcggat tatgcctccg ctcttaaatc gcttgaacgc
     2641 gctctcaaac tggctgagtc ggagggttat atccgcatat ttgtggatga aggtgcaggg
     2701 atggcaggtt tgctccgcaa agccctcgca aaaggaatca tgccagatta catcggcaaa
     2761 cttctaactg catttaaagt ggcagatcaa ggtttggggg tggaaatgtc tccttctgcc
     2821 gtaccagttt catcgtccat gatcgaagcg cttagtcagc gtgagttgga tatcctgcgt
     2881 ctgttcaaga ctgagttatc aggccccgag atcgcccagg aacttgtcat cgcattgagc
     2941 acggttcgca cacacaccaa gagcatctac aacaaactca acgtgaacag ccgacgaggg
     3001 gctgtcaaac gggcaattga acttggtttg atataagctc tctaccaaac agatttccaa
     3061 accccgccac atggttctgg cggggttttc gattctcacc ccatgaaatc cccacatgtg
     3121 gggaagacat ctccccatgt taatttgtat attgtcttca atcgaactca acaggcggca
     3181 acgccaaaaa cttaaaagga gactaccaat gaaaaccaat ggaaagacct cgatcaaaga
     3241 aagcaaaatg aaaatgacta cttcaaccct catgcggctg gcaggggtat ctgctgtatt
     3301 ggcagggctg tgcttcatca ttataggaat gttccatccg gagaatgtca cttcatcagt
     3361 tacaacaacc acatgggtga atgtccacat tgctgcgacc gctttgggtt tcttcggatt
     3421 gtttggcttg gctgggctct atgcacggca ggtggaaaaa tcgggctggc tggggttgac
     3481 cggctttctc ctatttagca tctggtttgt tctcgtatca ggtttttcgt tcgtcgaggc
     3541 tttcatcctg ccgcagttgg cagccgaatc gcctgcattt gtggaaagcc ttctcggaat
     3601 gtttaccaac actcccacca caattgacct tggagtcctg ccaaccttgt ggaatgtttc
     3661 agggcccatg tatatcctgg gtgccttgtt gtttggcatc gccaccttcc gtgcccaagt
     3721 tttacctcgc tgggcagcag gcttgcttac ccttggtgct gtgttgatcc ccatcggagg
     3781 aatggttccc cacgaatatc aggcaaagat cgcaatgata ccgatcggac tttccatggc
     3841 ttggctggga tccgcgctca tttttgaacg ccgtatgaaa gcatctgaat ccttgatctc
     3901 ccaaagaatc agcacaccag aggcaagcaa agttgcttga gattaatcat agagacaagc
     3961 ggttgcctaa ccgtttgtct ctaaaagttc ataaataatt tcaaggagtt aatgatgaaa
     4021 gcaatgatcc aggaaaatac tcaaatcaag atcacagcca gaaaagctca gacatcatgg
     4081 tggatacccg ctgggttgat cttactcagt gtgcttccgc tgacgttcgg agccatccgt
     4141 ctaagccagt tgataaacgg tgcagaaatc acccctgcca atgcccgctt ctttgccgcc
     4201 cctttgcctg ttgtcattca tattatcagc tcagcgatct atgccgtatt gggagcattc
     4261 caattcgtaa gccgactctg gcaacgcggg atcaaatggc atcgttgggt cgggcggctt
     4321 ttggttccat tcggtttgtt tgttggattc tccggacttt ggatgacctt attctatccc
     4381 cgcccaacca atgccagcga cctgctttat gcactgcgcc tcttttttgg ctctggcatg
     4441 attctatcca tcatcattgg attcctctcg atcagacggc ggaatgttgc tgagcaccaa
     4501 gcctggatga cacgtgccta tgccatcggt atgggagcag gcacacaagt gttgaccggg
     4561 atagtcgggg ccctggtcat tggcgaagtg aatgagtttg aaaacgcatt gttaatgggc
     4621 gcagcctggg tgatcaatct tgcgatagcc gaatggaata ttcgcaggga tgccacaagg
     4681 cagatccgca ccacacaggt ttgaatatcg acatcaggtt tactgagtat ttataaaaca
     4741 aggagtctaa aatgaaagca atcgtagcaa atcaatatgg tggtcccgaa gttcttcaac
     4801 tcaaagaggt tgggaaacca cagccaaaag ataacgaggt cttgatcaaa gtgcatgcga
     4861 ccaccgtcac catgggcgat tttagaatgc ggagttttac cgtcccgccc gtgttctggc
     4921 tccctgcccg ccttaccctg ggtatcacca aacccaaaca gccgatctac ggcatggaac
     4981 ttgccggtcg ggtggaatct atcggtaaaa atgtaactcg attcaaaatc ggtgatcagg
     5041 tcttcgcatc gaccctcacc gaaaactttg gcgcttacgc cgaatataaa tgtctgccag
     5101 aaaaagcgat gatggccatt aaaccagcca atatcagctt tgaagaagcc gcggcccttc
     5161 cgatcggcgc aactactgca ttgcgtctgc ttcgaaaagg aaagatccag cgcggacaaa
     5221 aggtcatgat ctatggtgct tcgggaagtg ttggcactta tgccattcag ctggcaaagc
     5281 atctcggagc agaagtgacc ggggtttgca gcaccgcaaa tctggagatg gtgaaatctc
     5341 tgggcgctga ccatgtaatt gattacacca aagagactct ttcagcaact aaagagcgct
     5401 acgacgtcat cttcgacacg gtgggaaaat tcgcgaaatc aaaggcctcg cagatcctta
     5461 tgccaaacgg ggcttttata acaatggcac aactggactc aaaagagagc atggataatc
     5521 tgatcttcat caaggaactg gtcgaagcgg gaaaactcaa agtagtcatt gaccgctgtt
     5581 atccgctgga agatatggtc gaggcacatc gctatgtcga ctcaggacgt aaaaagggaa
     5641 atgtcgtcat cacagtgggg aacacaaaat gacccttggg aaaatatcaa aacctgacct
     5701 ggatgaacca aggatctatc aaatcagagt aaaaggtcac ctnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nncttcaaaa tcccatcatc caatttttgc atcgttgggc
     5881 gctggatcct atatatgcca gagggtgttt ttaggcattc caatatcaat gctgcaccaa
     5941 aaaagagcgc agaatgcata acaggatggg ccactcatta cctgattggc acgatatttg
     6001 ccttcatttt tctggcagtt acaggcaacg actggctgca aaccccaagg atattccctg
     6061 ctcttatctt tggaacggtc accgtatctg ctccattctt cattatgcag ccagcgtttg
     6121 gacaggggat tgcagcatcg cgggcgggga atcctgctca ggcgcggttt cgcagtttga
     6181 taaatcacct tgtttttggc ttcggtcttt accttttcgg gttgatggca agctggatga
     6241 catggaaatg gactttcgct tgaaatacac aaaagtattg cggtaaaatc cgcccggaat
     6301 ttgtaagatt ttacagttac cacctatcca ttccataacc aactcaagga gagatctgta
     6361 atgaccactg ttcgtaacga tgtactcaaa attggcgata aattcgcaac cctgcttggc
     6421 gatgaagtaa gtgtggggca ggccgcgccc gaattcacct cggttgttcc aggctgggcg
     6481 cctgtcaatc cgatccagga atccaaaggc aaggtgatca tcctttctgc cgtaccctct
     6541 ctggataccg atgtatgcga ccgcgaaacc cgccgcttca acgaagaagc ctcaaaattg
     6601 ggcgatgata ttatcatcta caccatcagc accgatttcc caatggcaca aaaacgctgg
     6661 tgcggcgccg ccggtgtgga caaggtaaaa gtcgtttccg atgtacttga gaccgaattt
     6721 ggcttgaagt acggccttgt catcaaggaa cgccgcttcc tgcgccgctc ggtcttcatc
     6781 gtagggcgcg acggcaagtt gacctacgtc aaatacctcc ccacccttgg cgaggaaccg
     6841 aattacgacg aagtcatcaa cgccgccaag gaagccctaa aataaggttc ggccaaatta
     6901 caaaaaattc cgtctctaaa aaagacggaa tttttttgtt tccaaagcga aatcaacctg
     6961 gcactttttc agacggctgt cttagccgta cttttttctc aactcatctg cggcggaatt
     7021 caattcatct gcgttctttt ggatcttttt gagcgcagca atcacatcat ccacaccttt
     7081 tggaccttcg cggaagatcg cctcgtccag ccatacagct tcatgctcac aggcgcgggt
     7141 ggcttccggc aggttcatat ttttgaaatc aaaatattcg gggaaggcat ttggaacagc
     7201 caacttcgat ctctgtggct ggaacagttc atagttgtgc atcgcctcat acccaaccca
     7261 gcaatccact ccctcggcgt caagcgcgcc gcacaacaca tcatgttcta cgccaaattc
     7321 ctgtggatca atggcaaaaa tataacgata gaacgaacgg gtggtgtgac gttcatcacg
     7381 cttcatcacg cgcactcctt tgatctcgct cagggcctca tccatgtaag cggccatttc
     7441 ctcacgctgc ttcgcctgcg cagggaagcg ttcgatcccc accaatgcca tggcggcctg
     7501 caattcgctc atgcgaaaat taccgccctg catcgcgagt ccgttcttat cttctccgcc
     7561 gccgcccaac gcatgcggac gtccgcagtc gatcacagaa gcagccagag cagcaaactc
     7621 aggcgtacga cacagcaaaa taccaccctc gcctgccgtt agggttttcg aagattgcaa
     7681 actgaacgag ccaaagtgac cgattgtccc agcacctttg ccgcgccatt ttgcaccgtg
     7741 tgcatgagca cagtcttcaa tgacgatcag attatgtttt tcagccaact ccataatggc
     7801 gtccatgtcg gccatgttcg aaccgagatg cacagggatg atggccttcg ttttgggtgt
     7861 aatggccttc gccgccgcct ttgcatcaag acaataggta ttggggtcca catccacgat
     7921 tacagggatt gcccccgccc ccatcggcgc cgatgccgtg gcctgaaatg tataagcagg
     7981 cacaatgacc tcatcccccc aaccgatacc tgctgcacgc aacgccactt ccattgtgac
     8041 cgtgccgttg atcatcggca ccgcataccc acccccttgc agttcggcaa atttctttgc
     8101 cagttcggcg gtctttggcc ccgggtaggg gtagccgccc catcgccccg atttgaccac
     8161 atcgaccacc gcatcaatat cgcgttgatc ccacactggc cactgagggt atgcttccgt
     8221 ccttgttttg ggtccaccga gtatcgctaa ttcagacatg tgatgttctc cttttttaag
     8281 aagcctgcaa ttgaatcgca ggttttcgtt gtgaaatgac caacgccagc cacgcaagga
     8341 tcgtccctgc ggcggcgatg atgtaaagtg gataagcacc cacccaatcg aagatggctc
     8401 cgctgagggg ggcattgaac agtgccagga tgccattgag agtaacgtaa taaagcgaga
     8461 ggatggtggc accctgacct tccggtgcat tttcaaccgc atagcccact gaggcaatgg
     8521 catagaaact gtaataaaaa ccactggcag cacgcatgaa catgatggtg gcaatggagg
     8581 gatggatcac aataatgaac agcgagatgc cttcaatcaa tattgacagg gagaggatct
     8641 tcccagagcc atatttgcgg acaagtttgt ctgcccataa cataatgggc aattccatca
     8701 aggcagggta ggtgtaggcc cagccgatga gctgctcgct tgcaccaagt tgatgcatgt
     8761 agagtgtttc aaattggtgg cgtccgccat tggccagcca aaaaatagac aaggcaatgg
     8821 taagcccgag cagggtatgt tgggtgaata atgaccgcgc gacttttcca agagggagat
     8881 gatgttcctc ggaggcagaa gcaggtttcg gcgttgtgat gatgcgtccc aatatgaaga
     8941 tgcagagtcc gtacagggcg gcatatcccc aaaaaatgga gacgagtcca gcacggctga
     9001 ccatccaccc gccaataaag gcagtgatcg cccatcccaa tgaaccccat gaacgaatgg
     9061 agccgaagcc ttccttttcg ctggagatgc tgagcgcttg tgcatcggaa agcggaaaga
     9121 cgggtgcgcc gacgaacgct tcgaccgcta tgatgacagc catgatgata aaaagacttt
     9181 gttgaccaag gatgagcatc aatacgcctg aaccgaagag gcacagttgc agcacacggc
     9241 gcggattggc cgcattgtcg ctgatgcgcc cgataaaagg tgcgctgacc aggccaataa
     9301 ggctgccaat ggtggtgagc catcccatct gtgtgccgct taatcctttt tgtgcgtaaa
     9361 agaggctgat gaatgggaag ataaaaccgc ctgcaccggt ccagagcagg taatagacac
     9421 gcattaaaaa aagggagcgg gtagtgggtt gcataaaaat ttcaacgatg aatacggctg
     9481 atgcgctgat cgctgccgta cattgtttat tctccaaaaa agaaaggcaa atactgcttg
     9541 ttcgcttcaa gcagttcacg gagtaattgc tttgcctgcg gtatggtgag cctgctcgaa
     9601 agcggatcgt tgagcagaac ttgcaacgcg aggtcccaat cacccgtcag ggcagattgc
     9661 acggtgagtt cctgattgga aatatgcttt tgcaaaacac tttgcacacc agctgggact
     9721 tctccgtatg cgattgcgtg agcgccagta ttgctaatca ctccataagt ttcgacaaca
     9781 gcaatgttgg gcaagttagt aacctgccca acattgggga gattcataat tccaatatag
     9841 ttttctccac tcgtcactgc gcggatgatc ttattggccg cctctgtgga gatatcttcc
     9901 atgaacggct tcaaatcggt ttcaccgttc agcaccgatt cgatcattgt ttttgcaaag
     9961 gcttccaacg cctcgcggtc tgcgatgttg gtcagcttca ggtcgtaatc tgcaccccaa
    10021 tgagtgagtt cgttgatgaa gtgcggaaaa aattcggcaa tgtgccgatc gcctgcggca
    10081 ggcaatgcgc cgtacaactg aaacagcgtg gactttacct ttgcatggtc ggtgaacacg
    10141 gaggtatctt cttcatccac tttgatctcg ccgctcaaga ttctttcggt gatggctggg
    10201 agttcatccc aaacccgttt cccatcggcg tacaggtctg tgacccagat caggtggttg
    10261 acccctgcga tctgcgcctg aattttttca ggggatgtac cgaagatcag cgccagcttt
    10321 tcgcgcacgc cgatccactc gtggcacaaa ccgaccacct tcactccctg catggcaagg
    10381 gttcgtgtga gaacggtcat tgggttggtg taattcagaa aaagcgcgtt cgggcagatc
    10441 tccatcactt tgcgcccgat ctctgccaca acaggaatat tccgcaaggc ccgcgccaac
    10501 ccgccagggc cagtggtatc ccccaccgat tgcttgatgc catactttgc ggggatctcc
    10561 aaatcagggc gcatggattc gagcctgcca gtcgtgatcg tgaggatcac aaaatctgca
    10621 ccctgcaggg cttcttcaag cgaaagcgtc ttttccagtt gaaaatccag accgaaatca
    10681 tgcagcatct tttgaccaag cgcatacacc agatccacac gatcttgaag gatgtcgtgc
    10741 aagacaaggg ttgagccagc caattcaggt gtggcgaaaa gatcccgcat gaaagtgggt
    10801 ccccatgtgt acgaaccgcc gccgatgatt acgattttgg gcatgtctta ttatggtgat
    10861 gagtaatggg taaatggtaa ttaccaatta ctatttactt ttgactcacc aactccttta
    10921 atgaattcat aaccttcaac gaaatatcct tcccaatgga attcgtctct tcataatgtt
    10981 tgccaggctt gaatggattc cacggatatt tgaaatcttc cacaggcagg atgtagccaa
    11041 cttcatcatt tgccagcccg atcacccctg tgacgtgtgc ccccgcttct ttcatccacg
    11101 ccttgagctg caagcccaac tttggcagca actccccggg aacagttgcc agccacaacc
    11161 cgccgatctt gatcagattc acttcgctgg tgataaatcc ctttttatcg cgcacatccg
    11221 gcagaagttt tcgcccaaag gcaagtttgt acaaaatgtt ggtaagtttg gctttgatga
    11281 tgcgtttttc aaaactgacc gatgtcaacg gaccactgtc aactgcacga agcgccttca
    11341 ctccctcctc ccccaacttc ctccccatga actctgcctc ttcgaatgaa tggtctttca
    11401 catcgggggt catcatgccg cccagcgcac ctgcaaagaa aatacaggga gcgcctgttt
    11461 gcttttccac ttcctcccgc aggtagccga cataatccgc tgtaatattt gggttgtgtt
    11521 cccacaacac ctcagggtgg cagggaaaat tgaacaaagt tgccagcggc ttatcgtcgg
    11581 cagtggagaa ctgcaacaac gtcaactcgt catcgagaat ctccgggttg cgggcattct
    11641 tcgccaatcc cggaacgtgg agggatgtcc attttgcaga agcaggtttg aggtctgaaa
    11701 gcgaagcgat gaccgtatcc acgatcttct ttttaattgc agacatgtat ccctgatccg
    11761 cgccgcaggt tttgtcatca ggcccccaaa gacccatcgt atcggggcca tgatgggtat
    11821 gcgtggaagc gatgacaatt tgcaaatctg ccgcagagct tttcaaagca tttacgacct
    11881 ccaacacatc gtgtcgaaaa aatccgatca ggtcaagtgc aacaagtgcc aatgtgatct
    11941 tttcggtttg aatcgtcaat gcgcgcacat acagatcatc atggacggtt tctgcgcgac
    12001 ggttttgtcc aaagccagca agatacacag gtttgtcaag cgttggcgta attatttgtt
    12061 gggcataacc aatgttcata tcgaatcctt attttgacag ttactttaca atttccttcg
    12121 ccgtccgcac gatcacatcg tgcacggcat aaccatcgaa gccagcctgt tccataccga
    12181 ggatcaccgc accgaccacg ggcgggccat cgagccgaat caactttgct cgcgggcaat
    12241 gcttcaacac cacatcgcgc attggcttta tgatgatctc gcctgcctca aagatgctcc
    12301 ccgattgaac gatctccact tcatcattct ccatttcgat ctgccgcgcc actgaaaccg
    12361 ccagccagcc caactcctcc cccgcccatc gaacgatctc ctgcgcggcg gcatcaccgt
    12421 tccgcgccgc gttgatcacc tcgatcgcga taaaagggaa aatatgatat tgactgttcg
    12481 aaacgccttc catcaaatcg gcttcgtctt ttgcacctgt ggcttcaagc aaaacttttg
    12541 tcaatgctgt gggcgggttg cgtttaatcc atgcatagtt cacggcttgc agtgcgcgat
    12601 aggcaatttc aattccgccg ccatattcac caaaggtcgg gccattgcca acgatgcggc
    12661 cttctttgcc atgtgcattc cgtccgcggc aattgttgga ggaacccgcc gtcacattga
    12721 ctccgatgcc gcgcgttgca ccagcaagta agccgttcgt gccatcgttg acgatttcca
    12781 cagggcagga taatcccaac tttgagatgg cttgcaaatg cgattctctg tcagacggga
    12841 aatcgtatcc agccacgccg aagcctgctc ctgttatctg gtcattccca accccagaca
    12901 tttcctttgc accctcgaac gactcttgca agacattcaa caaaccatca tagccgacgc
    12961 cttgatgatt gccgccccag gattttccaa accccacaca ttgacctgtc tcatcgacaa
    13021 tcagcgcatg agtctttgac gaaccgacat ctattcctaa aaaatatttc ataaatctcc
    13081 gtttgtttga agggtacaag ttacaggttg aaggtttcag gttg
//
LOCUS       DAOQJC010000111        13039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2335_length_13039_cov_9.108133, whole genome shotgun sequence.
ACCESSION   DAOQJC010000111 DAOQJC010000000
VERSION     DAOQJC010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13039
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2335_length_13039_cov_9.108133"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1370)
                     /locus_tag="PLE39_14770"
     CDS             complement(174..1370)
                     /locus_tag="PLE39_14770"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNE69643.1"
                     /translation="MPEFAKRMAYMQSTADVIRYLFESMTDPDVISFGGGAPAGEALP
                     VELVHEIAKEVLVREKRGVQALQYGNPIGIPDLRQAVIDHLLLPKGLQVGMENVMIVA
                     GGMETMNLVSQLYLEPGDIVLVESPTFLQSIQIFKMFEARCIACETDDQGLVIDDVET
                     KIKQYHPKMIYVVPTFQNPSGRTTSLERRKALARLGSRYDIVVLEDDPYCEMRYSGEV
                     LPPIKYFDETGNTIFANSLSKIFAPGTRLGYIFADAPIIRKFYDAKTATNSHTNVVTQ
                     ILCAEFFNRGLFAPHLQAIRNIHRERRDVMMDCIKSMLPAEVRSIYPDGGLFTWVELP
                     EHVDTTSMLEEAKQHKVAYMPGREFFVDGQPIRNNCMRVSFGGVPPEKIRLGMERLAE
                     VICSKL"
     gene            complement(1538..2170)
                     /locus_tag="PLE39_14775"
     CDS             complement(1538..2170)
                     /locus_tag="PLE39_14775"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNE69644.1"
                     /translation="MMPHENSPVYAAGAKLENASAAMILLHGRGASAQDILSLASHLD
                     FPGIAYLAPQAEGFIWYPNRLIAPVESNEPYLSAAIAKIDRLVKQVEASGIPAEKIFI
                     GGFSQGASLSSEFVIRNPRPYGGLLVFSGGYIWQSGKPRETSGDLGGMPVFIGCSNVD
                     PFIPLERVKETSALLASMNANVTEQIYPNMDHTIIDEEIDLARQLLERRL"
     gene            2320..3708
                     /locus_tag="PLE39_14780"
     CDS             2320..3708
                     /locus_tag="PLE39_14780"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNE69645.1"
                     /translation="MNNQLKNDLDNLSSLLEQTRQASENYLRELAIRPPAAAYSKKEN
                     LKISVQGIGAEKTLQMFLERFGDHMPASNGPRFWGLVTGGTTPASLLGDWLTSVYDLN
                     LSHAGNSVAPDIEMEAISMLRELFGLPASFSGTFVTGATMSNFAGLAIAREWIGRQHG
                     KSVSQEGLQAIPPVRILSCEAHASSFKALGMLGLGRNSLKVIPRLPENREAVDVIELR
                     KALEELNGEPCIVIANAGTVNTVDFDDIEAIAAMKSKFKFWLHVDAAFGGFAACSPKF
                     EHLLKGIEHADSITIDAHKWLNVPYDSAMIFTQHRDLQIAVFQNSGAYLGAIAEPVDF
                     IHLSPENSRRMRALPAWFSLMAYGREGYRDIVERNCETAEQLGMKISASKEFRLLAPV
                     RMNVVCFTLHDAVDTERVKTFLANLRDDGRVFMTPTTYMGTPAIRAAFSNWRTTETDL
                     EIAWQAMTKHAA"
     gene            complement(3769..7257)
                     /locus_tag="PLE39_14785"
     CDS             complement(3769..7257)
                     /locus_tag="PLE39_14785"
                     /inference="COORDINATES: protein motif:HMM:NF013402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HNE69646.1"
                     /translation="MLPSRLAFENAVSFNQKHGHENLGFVSAQWGFLPVKPPLLALPD
                     YFKAWDVIAADLPSLFRTLQVRRAVDALPYLNVDEHHLPAEYLLRASSLVSILLHAYV
                     RVEASAPATLPSHLMQAWEGISQRLDRPAPILSYVDLILYNWKLRDPQASPLMLVENL
                     DLLFPTVGNEEERVFYLTQLEIAAACAPLMLAVIHAQEAASRDDVPALERELHLMLDV
                     FRHASEFSVHKIDPNPYSRTYVDQVVWAKTVAPFAVPFIKGRVGPSGTAAPIFMYMDA
                     FFGRESHASTLGRETENLLTYSPLHWRTLVEAVGKFSVRNYIARSGNRALQGLFDTLL
                     EAYAGDKGYLGTHRLKAYGFLEIAFKAGRSLTIGGFKGLFRDKTWNQIDDELGHARNE
                     RYLGLTPHLHSLHLEKGESSLPVSRNWITSLTLETRSDEMHYRPGDRISLLPENSPEL
                     IDRTLKALHAVGTESIHLNHMWQAHLQFRSGYSHETNSLPLSDLLRFGQIRPVNRATA
                     KALFKITGSSFLGRILNARMEDQWELWDLLQMLERGGFDPRQLWKAEPWEPEHITRLV
                     RPELPRLYSIASSPSLKKKLEISVAGLEYDSVESPVSNQFPRQGVASSFLRRLAEQAE
                     GQTELTVRLSPSARFHLPADADRPIVMFAGGSGIAPFAGFLQERMQSDLSRNWLYFGT
                     RTEAEIHHRDLLEKLAGAGRLHLRVALSREDKQIFLQDGRLAARSGTACHVDSLLDQD
                     AETLWSLLRNSDEGGEQACFYICGRTGFAGSIISALRNVIRRFVDSDSKADDFFRQLM
                     ADRRIMLDIFTTYGGTSDQAGRVFDISDLVLHNNDSNGYWMALNGKVYDLSEFVHLHA
                     GGRRILANNAGIDATRAYQSVQHHLNSDVDAQTGMYEIGRMRRLDFGMRWGVGLGLQG
                     LVFFTLEEAFTFWVRYLYLVTEMENALINEFSFLDRSTTLGEDPREFTPLKAHLVAEA
                     QKRFLGTFLDGILGEDLNVLWAITAGLCDRRSHYNWMPTSIQDTTLSASALHARANYL
                     QTWIDEWRSQDEEVHKTRFEQAVTQLSSIIRAENLRCFHEIKLAVRNGVQAFERHEAL
                     VIEKDGAGLISTLQQIPVIVSSYYERLGGEMQKLAQEYGVPLKSVVNSAQVDFQDLGH
                     GAHMDVTANPRIDTDG"
     gene            complement(7278..>7751)
                     /locus_tag="PLE39_14790"
     CDS             complement(7278..>7751)
                     /locus_tag="PLE39_14790"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE69647.1"
                     /translation="NNISSLWSDCMLVRDLMTIYPVSISIHSDLRRAAEIVSLAGASD
                     LMVVDTERRFLGVLSEGDLIRAIMPNFDEVLEAGGTLDDAFNFFIRKGKDLASRPIEP
                     LVIKNPIVVSPTDEAAQVATILIQKQIRRLPVVDNQLLIGTVSRADICRAVIYSA"
     assembly_gap    7752..7851
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7854..9848)
                     /locus_tag="PLE39_14795"
     CDS             complement(7854..9848)
                     /locus_tag="PLE39_14795"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE69648.1"
                     /translation="MIALLWRQNHRSLNGLGFWLADFVLQFTGLVLVSLREILPDFFP
                     VIVANTMVIGGTILLYIGLVRFLDTRRPQIHNYIFLALFVSLQVYFLEIHPSLEARNI
                     NLSAGLFFICIQSAWLMLRGKKIVSPPERGVGLIFLAYCFVSMVRIMTYVVDSPGENF
                     FAEATAFDAISILTYQMLFIALTFSLVLMVNQKLADEAAASMDMLHNSREILRFSEEK
                     FSKAFHASPDAILISRASDGKIIEVNEGFSRMLGYSHEEALTKHNLILQLWADPKDWE
                     NVLGLIRENDHVHNCEYGFVTRSDQKLYCLYSGEYINLGGETCILSIVRDITERRLVE
                     AELRESEMKYRLLFETMAQGVVYQDKDGQITFANPAAEKILGLTLAQMQGRASIDSRW
                     NAIHEDGTPFPGETHPAMVALRDGVAVFNVVMGVYNPQTDERHWININATPEFKPGEL
                     TPYQVYATFEDITQRMLVEEELRKTNELLHSQLDEIQSLHNALREQALRDPLTGLHNR
                     RYLNETMGREIARAKREKYPISIMIVDVDNFKQFNDTYGHSLGDKVLVAFGKVLHENT
                     RVGDIACRYGGDEFLVVMPGADIMGASQRAEDIRQKFAATPVYYGDSEIFATISIGLS
                     TYPQHGIDSSLFRSADEALYHAKHSGRNRIFIWSEKKMFG"
     gene            complement(10018..12978)
                     /locus_tag="PLE39_14800"
     CDS             complement(10018..12978)
                     /locus_tag="PLE39_14800"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNE69649.1"
                     /translation="MSKLIVSYSRKDSVVARKLIDTFKKDEFDVWVDWEDIPPAVGWL
                     EQILRGIEESDAFIFLISPDSVNSEVCKVEIEHAAKNNKRIIPIVLREVDPKTVIPTI
                     RDLNWIFIREQDDFAAGIEKVKVAITLDIEWVEEHRRLQVRALEWHREKDPSHLLRGH
                     DLRTARQMVASAESKDPKPTELQRTYIKYSINDETRRTTLFISAATALLVMIILSITA
                     FYQSQKASANEKLAQEQKLLAQNNEQLAKKNAETAEKNAAIAVKNQEVAEQNQLIAEA
                     QRSAARAQIYQSRTGGLYISTLLALDSMRRSPSQEAEDILRKNISLLPVPVAQMEQDD
                     IINALEISSNDDAFVTASADGTVCVWNLPDGKMRFCATSPEAVEDAAFSPDGKLIATG
                     DTSGVVMILDAQSGEIQNTYEYGVPIWDVNISPDGKSLAAARDDGRITFVSLVNRKFS
                     FELITFGGLYVTAFSPDGTWIAAGSNEGTITFWNLGNGKVLNGPSHHGEVFEIAFSPD
                     SNRLISGGSDSVAYMTLVSTGEELFKIINEDWVEDLTFSPDGKWFVTVSDDQRIRVWD
                     TATGKEKLRMLQDSFLSSVEVSPDGQWLATTGYDKTVRVWNAATGAEMFQIPINANGN
                     MLAFSRDGKYLISGDQAGEINVWDISALPASSSYLQMGALASNVQYSPSGEWMVASDG
                     NEVWLLTKEQLAAQSGLLKGSPTFEIDSLIQDLIISPDSKWVAVTTDDAEVILYNVAS
                     KSKKLVTEFNDVPSIVFSADGSQLITGDYDGSVQAWDTASGALVADLFDTDQNVNFLA
                     INAGQLAIGMQDQIVILDAATGEAISTIESPGDHQLLAFSADGSLLAANNTSGQVYIW
                     QKQAGGFDLLQNIPSEQAASMIFNPKGDRLLIGALNNVFVLDPFTGGEITRIRHKDTV
                     NSMSYSVDGVTLATSSFKAIQFWDMQKMSQLQGDDLIKAACNRLTQNFDTAQWTTFFG
                     EEPYQVLCENLPVP"
BASE COUNT         3476 a         3328 c         3012 g         3123 t
ORIGIN      
        1 agatcggctc gcgcatgaaa gccaccatct ccgttgacca ccgcgtcagc gacggagcgg
       61 aagcggcaca gttcatgcag aaaatggccg agttcctgga aaatccagtc agaatgttgg
      121 tataaaagca aacttccgaa gccaatggct tcggaagttt ttattttgaa atcctacagt
      181 ttcgaacaga tcacttcagc caaacgttcc atcccaagcc gtattttctc ggggggaacg
      241 cccccaaaac tgaccctcat acaattattg cggatgggct gcccatctac aaaaaattcc
      301 ctgcccggca tgtaggccac tttatgctgt ttggcttctt ccagcatgga ggtggtatcc
      361 acgtgttcag gcagttccac ccatgtgaac aaaccgccat ccggataaat agatctcacc
      421 tctgcaggca gcatggactt gatgcaatcc atcattacat cgcggcgctc acggtgaata
      481 ttgcggatgg cttgcaagtg cggcgcgaac aacccgcgat taaagaactc ggcgcacagg
      541 atttgtgtaa ccacattcgt atgggaattg gtggcggttt tcgcatcgta aaatttccta
      601 atgatgggtg catccgcaaa aatatagcca agacgggtgc ccggagcaaa tatcttggaa
      661 aggctattcg caaagatcgt gtttccagtt tcgtcaaagt atttgatcgg cggcagcacc
      721 tccccgctat acctcatttc gcaataggga tcatcctcca aaaccacaat atcataacgg
      781 ctccccaaac gggccaatgc tttccgacgc tctaaactgg tggtccgccc tgagggattt
      841 tgaaaagtgg gaacgacata aatcatcttg ggatgatatt gtttgatctt ggtttccacg
      901 tcatcaatga ccaacccttg atcatctgtc tcgcaggcga tgcagcgggc ctcgaacatt
      961 ttgaatatct gaatggattg aagaaatgtc ggcgattcca ccagaacgat atcaccgggc
     1021 tccaaataca actgactgac caaattcatg gtttccattc cgcccgccac gatcatgaca
     1081 ttttccatgc caacctgtaa ccctttagga agcaacaaat ggtcgatcac agcctgccgt
     1141 aaatctggga tgcctatcgg gtttccatat tgaagcgcct gaactccccg tttttcgcgg
     1201 accagcactt cttttgcgat ctcgtgaacc aactctaccg gcaatgcctc ccctgcagga
     1261 gcgcctccac cgaaagaaat aacgtccggg tcggtcatcg attcaaacaa gtatcggatc
     1321 acatctgctg tggactgcat ataagccata cgtttagcaa attcaggcat ggtgattatt
     1381 cctcgcttac ctcagaatat ttttggcccg aagtgtatca tttaaagagc atcgaaagta
     1441 ttctctggct tatttctgtt ggctctgcca gatattggat caaaaatcaa aactgcgacg
     1501 aattccgccg cagtttttca agtaaagaaa ggtcggctta caaccgtctt tccaacaatt
     1561 gtcttgccaa atcaatttcc tcatcaataa tagtgtggtc catgttcggg tagatctgct
     1621 cagtgacatt ggcattcatg gatgccagca aagcagaagt ctcttttaca cgttcaagcg
     1681 ggataaacgg gtccacattt gagcagccga taaagacagg catcccgccc aaatcgcctg
     1741 aagtttcccg gggttttcca ctctgccaaa tgtagccgcc gctgaaaaca agcagccctc
     1801 cataaggacg cgggttacgg atcacaaatt cgcttgaaag gcttgctccc tgcgaaaatc
     1861 caccgataaa gatcttttct gcaggaatgc cgctggcttc aacctgctta accaatcggt
     1921 ctattttggc aatggcagca gagagatacg gctcattcga ttcaacagga gcaatcagtc
     1981 ggttgggata ccagatgaac ccctctgcct gaggagcaag ataggcaatc ccggggaaat
     2041 ccagatggga ggcaagtgaa agaatatcct gcgcggaagc gccacgtccg tgcaggagga
     2101 tcatggctgc ggaagcattc tccagttttg cacctgcggc gtacacgggt gagttttcgt
     2161 gtggcatcat ggtcagcttc tcactttttc gaattgtgta attgaaaatt gaaaggtcat
     2221 tgggttttct cctaccccat agacgcgcca acattgaaaa atctcccccg gaattttaaa
     2281 aatattattc agttgatata ctatgaaaaa gaggtgttta tgaacaatca gttgaagaac
     2341 gatctcgaca acctatcttc tttattggag cagacaaggc aagcatctga aaattatctt
     2401 cgcgaattag ccatacgccc tcccgcagcg gcgtattcca aaaaagaaaa tctcaagatc
     2461 tccgttcaag gtattggtgc agagaaaaca ctgcaaatgt ttctcgaacg atttggagat
     2521 cacatgcctg ccagcaatgg accccgtttc tgggggctgg tcactggcgg cacaactcct
     2581 gcttccctgc ttggtgattg gttgacctcc gtctatgacc tgaacttaag ccatgccggg
     2641 aactctgtgg caccggatat tgaaatggag gcgatctcca tgctgcgtga gttgtttgga
     2701 ttacccgcct ctttctcagg gacatttgtc actggtgcga cgatgtccaa tttcgctggt
     2761 ctcgccatcg cccgtgaatg gatcgggcgt caacatggaa aatcggtttc acaggaaggt
     2821 ttgcaggcga ttcccccggt gaggatcctg agttgtgaag cacatgccag ttcgttcaag
     2881 gcattgggaa tgcttgggct gggacgaaac tccctgaaag tcatccccag acttcccgaa
     2941 aacagagaag cggtggatgt catcgagttg cgaaaagcat tggaagaatt gaacggcgag
     3001 ccctgcatcg tcattgccaa tgcagggacg gtcaacactg tagattttga tgacattgaa
     3061 gcgatcgccg caatgaaatc caaattcaag ttctggctgc atgttgatgc ggcctttggt
     3121 gggtttgccg cctgctcgcc aaaattcgaa catttattga agggcatcga acacgcggat
     3181 agcatcacca ttgatgcgca caaatggctc aatgtgccat acgactcagc catgatcttc
     3241 acccagcatc gtgacttgca aattgcggtc ttccaaaata gtggagccta tctcggtgca
     3301 attgccgaac ccgtggattt tattcacctc tccccagaaa actcccgccg tatgcgcgcc
     3361 ctgccagcct ggttcagttt gatggcatat ggcagggaag gctatcgtga tatcgtcgaa
     3421 cgcaattgcg aaacggcaga gcaacttggc atgaaaattt cagccagcaa ggaatttcgt
     3481 ctgctcgccc ccgtacggat gaacgtggtt tgttttacac ttcacgatgc cgtagataca
     3541 gaaagagtca aaactttcct ggccaacctc cgtgatgatg ggcgtgtatt tatgacacct
     3601 actacatata tggggactcc cgccattcgt gccgcattca gtaactggcg caccacagaa
     3661 acggatcttg agatcgcctg gcaggctatg acaaagcatg cggcataaac ccaactatat
     3721 ttttaggttt gaaggcgcac aggttatatt tggcttcgat aaaactaatc agccatcggt
     3781 gtcgatccga gggttggcag ttacgtccat gtgcgcgcca tgcccgagat cttgaaaatc
     3841 cacctgtgcg gaatttacaa cggatttcag gggaacccca tattcttgcg ccagtttttg
     3901 catctctccc cccaaacgtt cgtaatacga actgacgata acggggattt gctgaagtgt
     3961 tgatatcaat ccagcgccat ccttttcgat cacgagtgct tcatgccttt caaaggcctg
     4021 cacaccattc cgcacagcca gtttgatttc atgaaaacag cgcaggtttt cagcccgaat
     4081 gattgaactt aattgggtaa ccgcctgctc gaaacgagtc ttgtgaacct cttcatcctg
     4141 tgaccgccat tcatcgatcc aagtctgcag gtaattggcc cttgcatgga gggcacttgc
     4201 gctcaacgtt gtatcttgaa tggaagtcgg catccagttg taatgacttc ggcggtcaca
     4261 aagccctgcc gtgattgccc acaagacatt caggtcttca ccaagaatcc catcaagaaa
     4321 agtgccgaga aaacgttttt gggcttcagc cacaagatgc gccttaaggg gcgtaaactc
     4381 tcgcggatct tcccccaggg ttgtggaacg gtccaagaaa ctgaattcat ttatcagggc
     4441 gttttccatt tcggttacca gatataaata acggacccaa aaagtgaacg cctcttccag
     4501 ggtaaaaaag accaatccct gcaagccaag cccaaccccc catcgcattc caaaatccag
     4561 gcgccgcatg cggccgattt catacattcc tgtttgcgca tccacatcac tattgaggtg
     4621 atgttgaacc gattggtagg cacgggtggc atcgatccct gcattgttgg caaggatgcg
     4681 ccttccaccc gcgtgcaaat gcacaaattc actcaggtcg taaaccttgc cattcaaggc
     4741 catccagtat ccatttgaat cattgttgtg caaaaccaga tccgagatat cgaaaactct
     4801 acccgcctga tcgctcgtcc cgccataggt cgtaaatata tcgagcatga tcctacggtc
     4861 tgccatcagt tggcgaaaga aatcatctgc ctttgaatca gagtctacaa aacggcgaat
     4921 cacattgcgc agcgcagaaa tgatgcttcc agcaaagcct gtacgtccac atatatagaa
     4981 acaggcttgt tccccgcctt catcagagtt tcgaagcaaa ctccataggg tctctgcatc
     5041 ctggtcaagt aaagaatcta catgacaggc agtaccagaa cgagccgcca aacgcccatc
     5101 ttgcaaaaag atctgtttgt cctcacggga aagcgccaca cgcaaatgca aacggcctgc
     5161 ccccgccagt ttttccaaca ggtcacggtg atgaatttcc gcctcggtac gtgtaccaaa
     5221 atacagccag ttacgtgaca aatcggactg catgcgctct tgtagaaaac ccgcaaatgg
     5281 agcaatccct gaaccgcctg caaacataac gatggggcga tctgcatccg ctggcaaatg
     5341 aaaacgtgcg gatggactaa gtctcacggt caattcggtt tgaccttcag cctgctcggc
     5401 caaccgacgc aggaaactgg aggcaacacc ctgccgcgga aattgattgg atacagggct
     5461 ttccacagag tcgtattcca accctgcaac actgatctca agttttttct tgagacttgg
     5521 cgaagaagca atggaataca gacgcggtaa ttcaggcctc accaatcgtg tgatatgttc
     5581 gggttcccac ggctctgcct tccataattg ccgcggatca aaaccgcctc tttccaacat
     5641 ctgaagcaaa tcccataatt cccactgatc ctccatccgt gcattaagaa tacgtcccag
     5701 aaaactggag cctgttattt taaacaacgc cttggcagtt gcacgattta caggacgtat
     5761 ctgcccaaaa cgcaacaggt cgcttaatgg caggctgttt gtttcatggg agtatccact
     5821 gcggaattga agatgggcct gccacatatg gttgagatga atggattcag tgccaacggc
     5881 atgaagagct ttcaaggtgc gatcaattaa ttcgggactg ttctcaggca aaaggctaat
     5941 tcgatcgcca gggcggtaat gcatctcatc gctacgcgtt tccagggtta aagaagtgat
     6001 ccaattcctt gagacaggca atgaactttc gcctttttca agatgaaggg agtgtagatg
     6061 aggggttaat ccaagatagc gttcattacg tgcatgtccg agttcatcat cgatctgatt
     6121 ccaggttttg tcacgaaata atcccttgaa tccgccaatg gtgagcgagc ggcctgcttt
     6181 aaaggcaatt tccagaaaac catatgcctt aagacggtgc gtacccagat aacctttatc
     6241 ccctgcatat gcttcaagca gtgtatcaaa cagaccctga agtgcccgat tccctgaacg
     6301 ggcaatatag tttcttacag agaatttacc aaccgcttcc accaatgtac gccaatgcaa
     6361 aggggagtag gtcagcaggt tttctgtttc gcgtcccaat gtagaagcat gagactcgcg
     6421 accgaaaaaa gcatccatgt acataaagat gggggcagcc gtgccgctgg gaccaacccg
     6481 tccttttatg aatggcacag caaagggagc gaccgttttt gcccaaacga cctggtcaac
     6541 atacgtgcgg ctgtatggat ttgggtcaat cttatggact gaaaattcac tggcatgcct
     6601 gaagacatcc aacataaggt gtaattcgcg ctcaagagca ggcacatcat cgcggctggc
     6661 agcttcctgc gcatgaatca ctgcaagcat aagcggagca caggcggcag cgatctccaa
     6721 ttgcgtcaga taaaagactc gttcttcttc gttgccgacc gtgggaaaca acaaatccag
     6781 attttcaacc agcatcaaag gagatgcctg cgggtcacgc aatttccagt tatataaaat
     6841 caaatcaacg tatgaaagga tgggagccgg gcggtcaagg cgttgagaaa tcccctccca
     6901 agcctgcatc aaatggcttg gaagtgtggc aggcgcagaa gcctccacac ggacataggc
     6961 atgtaacaga atactgacca atgaagatgc acgcaaaaga tactcagcag gcaggtgatg
     7021 ttcatccaca ttaagatatg gcaaggcatc tactgcacgg cgcacttgaa gtgtgcgaaa
     7081 caaggaaggt agatcagcgg cgatcacatc ccacgccttg aaataatcag gcagggccaa
     7141 caatggcggc ttgacgggca ggaatcccca ttgagcagag acaaagccaa ggttttcgtg
     7201 tccatgcttt tggttgaatg aaacggcatt ctcaaatgcc aatcgggaag gaagcatata
     7261 ttaaatggga cgaaatgtta cgcagagtaa atcacggcgc ggcaaatatc agcacgtgaa
     7321 acagtaccta tcagaagttg attatccacg acaggcaatc tgcgaatctg tttttgaatc
     7381 aaaatagtgg ctacttgtgc agcttcatca gtcgggctga ccacgatcgg gttcttaatg
     7441 accaatggct cgatcgggcg tgaagccagg tctttcccct tgcggataaa gaaattgaag
     7501 gcatcatcaa gtgtcccgcc tgcctccagc acttcatcaa aatttggcat gatcgcgcgg
     7561 atcaaatcgc cttcggataa aacacctaaa aaacgacgtt ccgtatccac caccatcaga
     7621 tcactggccc cggccaggct gacgatctct gcggccctgc gcaggtcgga atggatggaa
     7681 attgaaactg gataaatggt catcagatcc cgcaccaaca tacaatcgct ccataagctt
     7741 gaaatattgt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtttaccca
     7861 aacatctttt tctcagacca gataaagata cgattacgtc cagagtgctt ggcatgatag
     7921 agagcctcat ccgccgagcg aaagagggaa ctgtcaatgc cgtgttgggg ataagtggat
     7981 aggccaatcg agatcgtagc aaaaatctcg ctatcgccat aataaacggg agtagccgca
     8041 aatttttgac gaatatcttc agcccgctga ctggcaccca ttatgtctgc gcctggcatc
     8101 acgacaagaa actcatctcc gccatagcgg caggcaatat cgccaacacg ggtattctca
     8161 tgcaaaactt tcccaaatgc caccaacacc ttatcgccaa ggctgtgacc atacgtatcg
     8221 ttgaattgtt taaaattatc cacatccacg atcatgatgc tgatgggata tttttcccgt
     8281 ttggcgcggg caatctcacg ccccatggtt tcgttgaggt aacgacggtt atgcagtcct
     8341 gtcaaaggat cgcgcaacgc ctgctcccgc aaggcattat gcaatgactg gatttcatca
     8401 agctgagaat gcagcaattc gttggtcttt cgcaattcct cttccacgag catgcgctgg
     8461 gtaatatctt caaatgtagc atagacctga tatggggtaa gttcccctgg cttgaattcc
     8521 ggagtagcat tgatgttgat ccagtgacgt tcgtcagtct gcggattata gacgcccata
     8581 acaacattga acacagccac cccatcccgc aaagccacca tggcgggatg cgtttctccc
     8641 ggaaagggtg tcccatcttc atgaatggcg ttccagcgtg aatctatgga agctcgtccc
     8701 tgcatctggg caagcgtcaa cccgagaatt ttttcggccg cgggattggc aaaggtgatc
     8761 tgtccatctt tatcctgata caccaccccc tgcgccatcg tctcgaaaag caggcggtat
     8821 ttcatttcgc tctcccgcaa ttccgcctca accagcctgc gttcggtaat atcacgcaca
     8881 attgaaagaa tacaggtttc cccgccgagg ttgatgtatt cccctgaata aagacaatat
     8941 aacttttgat cggagcgggt gacaaaccca tactcacaat tatggacatg gtcattctct
     9001 cgaatcagac ccaacacatt ttcccagtct ttggggtcgg cccacaattg tagtatcagg
     9061 ttatgtttgg tcaacgcttc ttcatgcgaa tatcccaaca tgcgggaaaa accttcatta
     9121 acctcgatga tcttcccatc acttgcacgt gatataagaa tcgcatccgg gctggcatgg
     9181 aaggctttcg agaatttttc ctcgctaaag cgcaggatct ccctggaatt atggagcata
     9241 tccatgctgg cagctgcttc gtcagccagt ttctggttca ccatcagaac cagactgaaa
     9301 gtgagcgcaa taaaaagcat ctggtaggtc aggatggaaa tggcatcaaa cgcagtggcc
     9361 tcggcaaaaa agttctctcc gggagaatct acaacatacg tcatgatgcg gaccatgctc
     9421 acaaaacaat atgccaaaaa gataagcccc accccgcgtt caggcgggct gacaattttc
     9481 ttacctcgca gcatcaacca ggcagattga atgcaaatga aaaacaaacc cgccgaaaga
     9541 ttgatattac gcgcttccag gcttgggtgg atttccagaa aataaacctg caaactgaca
     9601 aacagggcaa gaaaaatata gttatggatc tggggacgtc ttgtatcaag aaaacgtacc
     9661 agcccaatat acaaaagaat cgtccccccg atcaccatgg tattagccac aataacgggg
     9721 aaaaaatcgg gcagtatttc ccgcaaactg accaatacca gaccagtaaa ttgcaaaaca
     9781 aaatcagcca gccagaaacc caggccattc aaagatcggt ggttctgccg ccatagcaat
     9841 gcgatcacaa ccgcacatat tgcgttacta agaatataac cagcaaaaat agtctttaga
     9901 tccagcaaaa taaggcctca tccaatatga ttgatagact atttgaaaaa caataaccca
     9961 tattgtgaca aagatacagc aggaacaatt atgtcaaaac aagaaaaaca aactcaccta
    10021 cggcacaggc aaattctcac acagcacctg ataaggctcc tcaccaaaaa aggtggtcca
    10081 ttgggcagtg tcaaaatttt gggtgaggcg gttacacgcc gctttgatca ggtcatctcc
    10141 ctgaagctgt gacatttttt gcatatccca aaattgaatc gccttgaagg atgaagtcgc
    10201 cagagtaact ccatcgacgg aataggacat gctgttaacg gtatctttgt ggcgaatccg
    10261 tgtgatctcg ccgccagtaa atggatcgag cacaaagaca ttgttcagcg caccgatcaa
    10321 gagacggtca ccctttgggt tgaagatcat ggatgcggcc tgttcacttg gaatgttctg
    10381 aagtaaatca aaaccacccg cctgtttttg ccagatataa acctgacctg aagtgttgtt
    10441 cgccgcaagc agggaaccat ctgcactaaa ggccagtaat tgatgatcgc ccggagattc
    10501 gatcgtggag atggcttcgc cagtagcagc atccagaatg acgatttgat cctgcatgcc
    10561 aatagccagc tgcccggcgt ttatagccag aaaattaaca ttctgatcgg tatcaaacaa
    10621 atctgcaacc agggcaccgc tcgctgtatc ccaggcttgc acagaaccat cataatctcc
    10681 cgtgatcaac tgggaaccat ctgcagaaaa aacgatggat ggcacatcat taaattctgt
    10741 aaccaatttc ttcgattttg aagctacgtt atataagatc acttcggcat cgtcagtggt
    10801 gacagcgacc cattttgaat cagggctgat aatgaggtct tgaatgaggg aatcgatttc
    10861 aaaggtcgga gagcctttca agagtccact ttgggcggcg agttgctcct tggtgagcaa
    10921 ccagacttcg ttcccgtctg aagcgaccat ccattcgcca gatgggctgt attgaacatt
    10981 cgatgccagc gcacccatct ggaggtaact actggaggca ggcaatgcag aaatatccca
    11041 aacattgatc tcgccagcct ggtcgcctga gatcaggtat tttccatccc tgctaaatgc
    11101 aagcatgttg ccattggcgt tgatcggaat ttgaaacatc tccgcacccg tggcggcatt
    11161 ccacacccgc actgttttat cgtatcccgt tgtggcaagc cattgaccat ctgggctgac
    11221 ctccacactg ctgaggaaac tatcctgcaa catgcgcagc ttttctttgc ccgtggcggt
    11281 gtcccatacc ctgattcttt ggtcatcgga aaccgtaaca aaccatttgc catcggggct
    11341 gaaggtgagg tcttcgaccc aatcttcatt gatgatcttg aacaactcct cacccgtgct
    11401 gacaagggtc atgtaagcca cgctatcaga accgcctgaa ataagtcggt tgctatcggg
    11461 gctgaaggca atttcaaaaa cctcaccatg gtgcgatggc ccattcaaaa ccttgccgtt
    11521 gcccaaattc caaaatgtga tggtgccctc attgctgccc gccgcgatcc acgtgccatc
    11581 ggggctgaag gcggtcacat acaagccgcc aaacgtaatc agctcaaatg aaaatttacg
    11641 attcacaagc gagacgaatg tgatacggcc atcgtcacgg gcggccgcca gagatttccc
    11701 atcggggctg atgttcacat cccagatcgg gacgccatac tcataggtat tctgaatctc
    11761 gccgctttgc gcatcaagga tcataaccac accagaggta tctccggttg caataagttt
    11821 cccatcggga ctaaaagccg catcttccac agcctcgggg ctggttgcgc aaaaacgcat
    11881 ttttccatct ggaagattcc acacgcagac cgttccatct gcactggcag tgacaaaagc
    11941 atcatcgttc gagctgatct ccagcgcgtt gatgatatcg tcctgctcca tttgcgcaac
    12001 aggcacaggc agaagcgaga tgttcttgcg aaggatatcc tccgcttcct gcgatggact
    12061 acgccgcatg gagtcaagcg ccaacagggt gctgatatac aaaccgcccg tccgcgattg
    12121 ataaatttgg gcgcgtgccg cgctgcgctg tgcttcagcg atcaattgat tttgctcggc
    12181 aacttcctga ttcttcacag caatggcggc atttttttca gcggtttcgg cattcttttt
    12241 ggcaagctgt tcgttatttt gtgccagcag tttttgttcc tgggcaagtt tctcgttggc
    12301 agaagctttt tgcgattgat aaaaagccgt tatcgaaagg attatcatga cgagcaatgc
    12361 ggtagcggct gaaataaaca aggttgtccg ccgtgtctca tcgttgatac tgtatttaat
    12421 ataggtacgc tgtagctcgg tgggtttggg gtctttgctt tcagcagaag ccaccatctg
    12481 acgggcggtc cgcaggtcgt ggccgcgcaa aagatgactg ggatccttct cacgatgcca
    12541 ttccagggcg cggacctgca gccgacggtg ttcctccacc cattctatat ccagcgtaat
    12601 ggcaaccttc actttttcaa tgccggcggc aaaatcatcc tgttcacgaa tgaagatcca
    12661 attcagatcg cggatggtgg ggatgacggt tttggggtcc acctcacgaa gcacgatcgg
    12721 gatgatgcgt ttgttatttt tggcggcatg ctcaatttcc accttgcaca cctcagaatt
    12781 gaccgaatca ggactgatga ggaaaatgaa cgcgtctgat tcttcgatgc cgcgcaaaat
    12841 ctgttcgagc caccccaccg caggcggaat atcctcccaa tccacccaga catcgaattc
    12901 gtcctttttg aacgtatcga tcaacttgcg ggcaactacg gaatcttttc gtgagtagga
    12961 aacaatgagt ttgctcataa gatgcctcta tgcagattat aacctttgaa aatataccgc
    13021 actcggtcac aaattatgc
//
LOCUS       DAOQJC010000112        12809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2404_length_12809_cov_10.595891, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000112 DAOQJC010000000
VERSION     DAOQJC010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12809
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2404_length_12809_cov_10.595891"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..249
                     /gene="rpmG"
                     /locus_tag="PLE39_14805"
     CDS             100..249
                     /gene="rpmG"
                     /locus_tag="PLE39_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNE69650.1"
                     /translation="MRPVITLQCSDCKERNYTTEKNRRNDPNRLEFNKYCSRCKKHTQ
                     HRETK"
     gene            325..397
                     /locus_tag="PLE39_14810"
     tRNA            325..397
                     /locus_tag="PLE39_14810"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:358..360,aa:Trp,seq:cca)
     gene            461..679
                     /gene="secE"
                     /locus_tag="PLE39_14815"
     CDS             461..679
                     /gene="secE"
                     /locus_tag="PLE39_14815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNE69651.1"
                     /translation="MAEKEKKVKKSDNAVQRYFRETSGELRKVSWPTWPEARRLTELV
                     LAVMVIVGFFLAIIDYVSDLLINLILGI"
     gene            699..1391
                     /gene="nusG"
                     /locus_tag="PLE39_14820"
     CDS             699..1391
                     /gene="nusG"
                     /locus_tag="PLE39_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNE69652.1"
                     /translation="MDLPESQEQFEQEPMGEQTEEQNTPAVVSVSSSDPKPVPASESQ
                     IPTDLPPVETIVEGPAWYVIHCYSGYENKVRHNLEQRIETMGMKDKIFDVVIPTQEEI
                     EVKDGKRRTVERHIFPGYVLVNLTLSEESWYVVRNTPGVTGFVGMGNSPTPLRPEEVA
                     QIVKRMEAEAPTVKVSFKVGERVRIVDGPFNDFRGVVSEIDMERTKVRVMVSFFGRET
                     PVELDFLQVEKA"
     gene            1494..1919
                     /gene="rplK"
                     /locus_tag="PLE39_14825"
     CDS             1494..1919
                     /gene="rplK"
                     /locus_tag="PLE39_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNE69653.1"
                     /translation="MAKKLKAIVRLQLEAGKANPAPPVGPALASHGINIMAFCKEYNA
                     RTSNRPGEVLPAEITIYTDGSFTFVLRTPPAAVLLRKAAKVEKGSGVPNKDKVGKVTR
                     AQVKEIAELKMKDLNAINIEGAMKQVEGTARSMGITVTD"
     gene            1970..2683
                     /gene="rplA"
                     /locus_tag="PLE39_14830"
     CDS             1970..2683
                     /gene="rplA"
                     /locus_tag="PLE39_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNE69654.1"
                     /translation="MAKHGKKYTAAAAKVDIDKVYTAREAVSLAKETSITKFDATIEV
                     HMRTTLDSRQADQQLRDVVVLPHGLGKTVRVLVFAQGEGAAAARAAGADLVADDDETM
                     KKIEGGALDFDVAIATPDAMGKVGRLGRVLGPRGLMPNPKAGTVVGAADMERAIKEAK
                     AGRVEYRLDKTNNVHVSIGKASFGADKLFENYVALMDAVNKSRPSGAKGNFVKRITIS
                     STMGPGIKTDPNEHIDGAE"
     gene            2904..3434
                     /gene="rplJ"
                     /locus_tag="PLE39_14835"
     CDS             2904..3434
                     /gene="rplJ"
                     /locus_tag="PLE39_14835"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNE69655.1"
                     /translation="MAISKQRKEEVQAQYADWVKRSEAVILVEYSGANMKSIDAIRSK
                     IRETGGEFHIVKNTLARRVFADNGMDVPADYLVKSTAVSFAFKDPASTAKALTDAIKG
                     NEFVKVKGGLMGGKSLSPAQVKALSDMPPLPVMRATLLGVLQAPAGKLVRTLAEPARG
                     LAAVIKARSESAQASA"
     gene            3507..3890
                     /gene="rplL"
                     /locus_tag="PLE39_14840"
     CDS             3507..3890
                     /gene="rplL"
                     /locus_tag="PLE39_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNE69656.1"
                     /translation="MSDKLAKLVDELSTLSVLEAADLVKMLEEKWGVSAAAPVAVAAV
                     AGGAAASAEPVEEKTEFDVVIKDAGAKKIDVIKVIRQLTSLGLGEAKTLAETAGGKVL
                     SAVGKDAAMDAKKKLEEAGAVITVE"
     gene            complement(3950..4624)
                     /gene="lexA"
                     /locus_tag="PLE39_14845"
     CDS             complement(3950..4624)
                     /gene="lexA"
                     /locus_tag="PLE39_14845"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNE69657.1"
                     /translation="MMVRKSKGLGERHQKILDFIASYQRDHKHPPSIREIGEHCDISS
                     TSVVNYYLDQLEKSGNIERDRKISRGVRLTGSTPLGDMFRVPVLGVIQAGEPIPVPSN
                     ADFNAFTPEDSVEIAMSLMPTKEKGKELFALKVQGESMIDAMVNDGDIVILKPTQDAR
                     NGDMVAVWLSDRNETTLKFFFKEKDKYRLQPANPTMSPIYVNKNEALEIKGKVVMVIR
                     KVERYM"
     gene            complement(4740..5726)
                     /gene="corA"
                     /locus_tag="PLE39_14850"
     CDS             complement(4740..5726)
                     /gene="corA"
                     /locus_tag="PLE39_14850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNE69658.1"
                     /translation="MIRSLFFSPGKPVRTNIPTEEFPRLLRDRRGLLWVDFEAEPEES
                     ALPILNGFGFHQLAIDDALQQTHAPKIDDWGDYLYIVLNYMHLTKATEPWDTEIDELD
                     IFLGRNYVITHHDNPITSINETWNTSQRDPRYSQDGADHLLYKIIDAIVMNYMPIIEK
                     IDEEIDWVEDQVFDRPSSQTLARLFTLKRVLLAMRRILLPQREVLNKMARDDFQVIDR
                     KDRIFFRDIYDHLVRLHDVNESLRDLVGGALDTYLSVINNRMNEVMKTLTIITTLFMP
                     LTFVTGFFGMNFFAANPPYESWTLPAVFYGTLSLMLLSPIIMFIWMRRHTWV"
     gene            5917..6156
                     /locus_tag="PLE39_14855"
     CDS             5917..6156
                     /locus_tag="PLE39_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69659.1"
                     /translation="MDILSERMSELKRKSIQEDLDAIHLEEEAVKGKTLLDKNLAWIG
                     NWMVSRGEKLRKRYNQSLHDANSTQLVKKAAYHAK"
     gene            6146..7402
                     /locus_tag="PLE39_14860"
     CDS             6146..7402
                     /locus_tag="PLE39_14860"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE69660.1"
                     /translation="MLNNTIEKATKLYHEYPRTFWILIVITFIDRIGGALLFPFFALY
                     LTSKFGVGMADVGVLFAAFSVSSFVGSAVGGALTDRFGRKGIIIFGLIASSFSTVAMG
                     LVTSFEYFFVLAFFIGILTDVAGPAHQAMIADILPEDKRADGYGILRVAFNLSVVIGP
                     AIGGLLAARSYLMLFLSDAAISLLTVILIAIFLPETKPEAHPDAPKESMTRTFAGYGQ
                     VFRNTAFMLFLGAVLLQVFTYMNMNTSLGVFLRNEYGINESGYGLLLSINAAMVVLMQ
                     FPITRRITNFPPMLMMAAGTFLYVIGFSMYGFVTTYAMFVAAMVIITFGEMVVSPVAQ
                     ALVASFAPEEMRGRYMAVSGFSWGIPFAVGPYLAGLIIDGPSPHMLWYAAGIVGTLST
                     VGFLVLYRVKSKQTQNLELMPEVTAS"
     gene            7419..7802
                     /locus_tag="PLE39_14865"
     CDS             7419..7802
                     /locus_tag="PLE39_14865"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HNE69661.1"
                     /translation="MRPAPTRCPICQSELTVARLHCSSCDTTIEGNFASGQFSNLTPE
                     QLEFVFTFVRCEGKINRMEQEIGLSYPTIRNRLHEIIRALGYEPGRDEPVEITQEKRN
                     SILDDLHAGKITADEATRLLRGEEE"
     gene            7806..8897
                     /locus_tag="PLE39_14870"
     CDS             7806..8897
                     /locus_tag="PLE39_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69662.1"
                     /translation="MSKTISAGRSPRVVIENIEGDLSLVGWEGDDILLKGDEDEMHIR
                     QDGNQVIISCDDDLSLRVPKSASVSIKKIGGDAAMRGVMGDIELNEIEGDLSIRDVNS
                     VSIGTIHSDFSLRGAKGSIAIKKVLSDVSIRDVDGNVSLESVADDLALRDVRGNVSAN
                     VAEDVVLYLDPKPGNAYAVTAGEDILLVMPPRANATLTLSADEIDIEWKGVDNDDDAT
                     SRVVTLGDGSALVTLSAGGDIRVTNQSNAGDSAEDFGNFAGIGMDWSGFGERISRQVE
                     QATKRATRQADEAIRRAAANVERRAKVNVNAKVGRWNWDLSPKGVPVTPPSQASEEER
                     MAILKMLQEKKISAEDAEKLLSALEGGES"
     gene            8897..9535
                     /locus_tag="PLE39_14875"
     CDS             8897..9535
                     /locus_tag="PLE39_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69663.1"
                     /translation="MSSEERRKILQMVADGKISAEEAAGLMRALDEDAGEEDSAGEQI
                     EVLQAESGFGPEQTEAPEFDEVRRRAFRFAMIPLWIGVIVTVLSAWGMYSVQMGAGLN
                     FWFFCLTLPLLFGVLLIALGAGGRASRWIYVNVDRSHQNEWPRKITLALPIPLGLVSW
                     FLKNFGSRIDGLKKTTVDDVLMAISMTKSVTEPLIVHVNDDNSGDRVQVFIG"
     gene            9545..9934
                     /locus_tag="PLE39_14880"
     CDS             9545..9934
                     /locus_tag="PLE39_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69664.1"
                     /translation="MANAEERMKILKMIEEGKISADEGSKLLAALSGPRKGVPTPPRP
                     PSVGNSTPRWLRIRVTDTRTGRSKASVQIPLALVDAGMKIGAHFAPEVEGVDMSNVME
                     ALRMGVTGKIIDVTDEEDGEHVEIYVE"
     assembly_gap    10122..10221
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10244..11176
                     /locus_tag="PLE39_14885"
     CDS             10244..11176
                     /locus_tag="PLE39_14885"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNE69665.1"
                     /translation="MRFAIFGTGGVGGYFGGKLAQVGEDVTFIARGKNLESLKNSGLR
                     VDSILGDFVLEKVQATDLPHSLKNIDVILLATKTWQLDSAIAEMKPMLGEQTSILPLL
                     NGMEHMDTLRAEFGGHVLGGLCRISSFLIAPAHVSHVAVNPHIEFNEWKQETSPRVGE
                     VLSAFSKIKEITVDTPPDIELAMWEKYLLICAFSGVGAITRQPVGVFRSDPESRAMFI
                     RSLHEVVAVARARGVNLTEKSAESVLARIDQTQPDMMASMQKDVMEGRPSELEAQIGA
                     LVRMANAANIPVPTNEFIYSTLLPLEQKARSKAT"
     gene            11282..12181
                     /locus_tag="PLE39_14890"
     CDS             11282..12181
                     /locus_tag="PLE39_14890"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNE69666.1"
                     /translation="MSKNIFVELSNAIADAAEQAGKFTVLVDARRRFPASGIVFAKDL
                     ILTADHVVEREEDINVILADGTEVTARLVGRDPGTDLAVLKLDSASATPAEVSKTPAR
                     VGQFVLAVGRPSSNGIESSFGSVNSLGGPVRTGRGGMLEKYIKTDVVSYPGFSGGPLV
                     NGDGTVLGINTSGFGNSGAITIPADVAWKIAEALAKHGKIKRGYLGIRSQVVELSADG
                     KKALKREQESGLLLVGLEADSPAGKGGLIVGDILVGIAGEPVAHQDDLFARLSGDVVG
                     KSTPVDVLRGGKLETLKVEIGER"
     gene            12182..12790
                     /locus_tag="PLE39_14895"
     CDS             12182..12790
                     /locus_tag="PLE39_14895"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE69667.1"
                     /translation="MIRVAVVSPNSALRVGLRELLGRQPDIQVIGEAVDLESVHEMEA
                     EVFVLASVSSGRLLENSITSPILFLTDDIESVRMVLGSKVRAWGVLSLEATEDELAAA
                     VRAVGEGLWVGAPGLVGEMIRFSGRRESLGEESLTEQLTAREKEVIQLMAQGLANKQI
                     ALSLGISEHTVKFHLSSLYSKLGISSRTEAIKRGIELGLISL"
BASE COUNT         3070 a         2896 c         3497 g         3246 t
ORIGIN      
        1 ttcgtgaagg cggtctcacc gtcggcgcgg gtgtcgttac caagatcatc gagtaattca
       61 ggaaagtaag gtagcaagat ggcttcgaag aagaaagaca tccgcccggt cattacactt
      121 cagtgcagtg actgcaaaga gcggaactac accaccgaaa agaatcgccg caatgatccg
      181 aatcggttgg agtttaacaa gtattgctcg cgctgcaaga agcacaccca gcaccgcgaa
      241 accaagtagt ttcatcgtca gatgaccgtg gagcaaaggc tgccctgata agtagctgat
      301 acaaagcggc catctgacta atttaggcca gtggctcaat tggcagagca ctcgtctcca
      361 aaacgagcgg ttgtgggttc gattcctacc tggcctgcct gtggcaaacg ccgcgctttg
      421 tgcggcgttt tagccgtaaa accatagaag gagtattacc ttggctgaga aagagaagaa
      481 agtcaagaag agtgataacg ccgttcagcg ttatttccgc gaaacatctg gtgaacttcg
      541 caaagtcagc tggcctacct ggccagaagc aaggcgtctc acagaactcg ttttggcggt
      601 tatggtgatt gtgggttttt tcctggcaat cattgactat gtgtcggact tattgataaa
      661 tctgatactc ggaatttagt cttgaataga ggaaaatgat ggatttaccg gaatcgcaag
      721 agcagtttga acaggaaccg atgggtgagc aaaccgagga gcagaatact cctgcggttg
      781 tttccgtttc ttcctcggac ccgaaacctg ttcctgcctc cgaatcgcag attcccaccg
      841 acctgccccc ggttgagaca attgtcgagg ggcctgcttg gtatgtgatc cattgttatt
      901 ctggatacga gaacaaggtt cgccataatc tcgagcaacg catcgagacc atgggaatga
      961 aagacaagat ttttgacgtg gttattccga ctcaggaaga aatcgaagtc aaagacggaa
     1021 aacgccgcac tgtggaacgt cacatcttcc ctggttatgt gttggtgaat ttgaccctct
     1081 ccgaagaatc ctggtatgtg gtgcgtaaca cccctggcgt cactggtttt gttggaatgg
     1141 ggaacagccc cacacccttg cgccctgaag aagttgccca gatcgttaaa cgaatggaag
     1201 ctgaagctcc cacggtcaaa gtttcattca aagtgggtga gcgggttcgc attgtggacg
     1261 ggcctttcaa tgattttcgt ggtgtggttt cagaaattga catggaacgc acaaaagttc
     1321 gcgtcatggt cagtttcttc ggacgtgaaa cgcctgttga gttggatttt cttcaggttg
     1381 agaaggcgta aagtagaaaa tattaattgg acagtagcag gcctcagttt ggcctgatgc
     1441 ggtgggaggg tctcccaaat gacccgctaa aaccacaaag gagttaagtc gaaatggcaa
     1501 agaaactaaa agcaatcgta cgtctgcagc ttgaggctgg caaggcaaac cctgcgcctc
     1561 ccgtcggtcc tgcactcgct agccatggca tcaacatcat ggccttttgt aaggaataca
     1621 atgcccgaac ttccaatcgt ccgggtgaag tactccctgc agaaattacc atttatacag
     1681 atggttcatt cacatttgtt ctgcgcaccc cgcccgccgc ggttttgctt cgcaaggctg
     1741 ccaaggttga aaagggatca ggcgttccga acaaggacaa ggtcggtaaa gtgacccgtg
     1801 cccaggtgaa ggaaattgcc gaactgaaga tgaaggatct gaatgcgatc aacatcgaag
     1861 gcgcaatgaa gcaggttgaa ggtacagccc gttcgatggg cattacggta accgattaat
     1921 tttgtgggag gacggctctg tgctgtccgt tcgtaccacg gaggataaaa tggctaaaca
     1981 cggaaaaaaa tatactgcgg cggctgccaa ggttgatatt gataaggttt acactgcccg
     2041 cgaagcggtg tcactggcaa aagaaacttc cattaccaaa ttcgatgcga ccatcgaagt
     2101 tcacatgcgc acgacacttg actctcgtca ggctgatcag cagttgcgcg atgtggtcgt
     2161 gctccctcac ggccttggta agacggttcg cgtgctcgta tttgcccagg gtgaaggtgc
     2221 tgctgcggct cgtgctgctg gtgccgatct ggtcgcagat gatgatgaga ccatgaagaa
     2281 gatcgaaggt ggtgcgttgg atttcgatgt tgccatcgcc acacccgatg caatgggaaa
     2341 ggtcggtcgt ttgggtcgtg ttctcggtcc tcgcggtttg atgccgaatc ccaaggctgg
     2401 taccgtagtt ggtgctgctg atatggaacg tgcgatcaaa gaagccaagg ctggccgtgt
     2461 tgagtaccgc cttgataaga cgaacaacgt gcatgtttca attggcaagg cttcttttgg
     2521 tgctgataag cttttcgaaa actatgtggc tttgatggat gcagttaaca aatcccgccc
     2581 atctggtgcc aagggtaact tcgtcaagcg cattacgatt tcttcaacca tgggtcctgg
     2641 catcaagaca gacccgaatg aacacattga tggtgccgag tagaaatact cgttaaaacc
     2701 taaaatacca cttcgccagt gaaggcgggt gccttgagat cttcaaggct taattccccg
     2761 ctcaggtgga gactgatagc aaaatatcgg catatttctg tgccggtttc cctcccagta
     2821 gggcttcccc tcgcggggat tgcaaaaagt ctttgcctgc gtaagtggca aagacttttt
     2881 atttgaaagg aggtgaataa cctttggcaa tttccaaaca acgcaaggaa gaagtacagg
     2941 cccagtatgc agattgggtc aagcgcagtg aagcggtcat tcttgtggaa tatagcggcg
     3001 cgaatatgaa gtcgatcgat gcgatccgct caaagatccg tgaaactggc ggtgaattcc
     3061 atatcgtcaa gaatacactt gcccgccgtg tttttgcaga taatggtatg gatgttcctg
     3121 cagattatct tgtgaagtct acggccgttt cgttcgcatt caaggacccg gcttccaccg
     3181 caaaagcatt gacggatgcc atcaaaggca atgagttcgt caaggtgaag ggcggtttga
     3241 tgggcggcaa gtctttgagc cccgcccagg tcaaggccct gtctgatatg ccgccgctgc
     3301 ccgttatgcg tgcaacattg ctcggcgttt tgcaggcccc ggctggcaaa ctcgtccgca
     3361 cgctcgcaga acctgctcgc ggtctcgcgg ctgtcatcaa ggcgcgttct gaaagcgcac
     3421 aggcttcggc ctagttatcg atttatcgtc gggccctgcc cgaaccataa tcaaatttca
     3481 aattatctag caaggagtgc tatacaatgt ctgataagct cgcaaaactc gtggatgaac
     3541 tttccacact ttccgtcctt gaggcggctg atctcgtaaa aatgctcgaa gagaagtggg
     3601 gcgtttccgc cgccgctcct gtggctgtag ctgctgtcgc tggcggcgct gctgcttccg
     3661 ctgaacctgt tgaagagaag actgagttcg atgtggtcat caaggacgct ggcgccaaga
     3721 agatcgacgt catcaaggtc attcgtcagt tgaccagcct cggcctcggc gaagccaaga
     3781 ccctcgctga aaccgctggc ggcaaggtac tctccgctgt tggcaaagat gccgcgatgg
     3841 acgccaagaa gaagctcgaa gaagctggcg ccgttatcac ggttgagtaa gaagcaagtc
     3901 ttaaataaaa aaggacggct cgcaagagcc gtcctttttt atttgtttat tacatgtacc
     3961 gttctacttt gcgaatgacc atgaccacct tgcctttgat ctccagagcc tcgttcttgt
     4021 tgacatagat cgggctcatg gtggggttgg cgggttgcag gcggtacttg tctttttcct
     4081 tgaagaagaa tttcagagtg gtttcgttgc ggtctgaaag ccataccgca accatgtcgc
     4141 cgttgcgggc gtcctgggtg ggtttgagga tgacgatatc accatcgttt accatggcgt
     4201 cgatcatcga ttctccctgg acttttagcg cgaacaattc cttgcctttt tctttcgttg
     4261 gcatcaaaga catggcaatt tcaaccgagt cttcgggggt aaaggcattg aaatcagcat
     4321 tggaagggac agggatgggt tcaccagctt gaatgacacc aagcactgga acgcggaaca
     4381 tatcaccaag cggtgtggag cctgtcagac gaacaccgcg ggatattttg cgatcacgtt
     4441 cgatgttgcc cgatttttcc agctgatcca gataataatt aactacagaa gtagaggaaa
     4501 tgtcacaatg ctccccaatc tcccgaattg aaggggggtg cttgtgatcg cgttgatagc
     4561 tcgcaatgaa atcaaggatt ttctggtggc gttcgcccag acctttgctt tttctaacca
     4621 tcatcatatt agcctccacg agcgcatttt tcgctcattt gtgctaggaa tgataaccga
     4681 acgcctgttc tgtgtcaagg atttttttag cagatttatg cggcaaacca tggggccgcc
     4741 tatacccagg tatggcgacg catccagatg aacataatga ttggggataa cagcatcagg
     4801 ctcagggtcc cgtaaaagac cgctggcagg gtccagcttt cgtagggagg attcgcggcg
     4861 aagaaattca tcccaaaaaa acctgttaca aaagtcaacg gcatgaaaag ggtggtgatg
     4921 atggtaaggg ttttcatgac ctcattcatg cggttgttga tgacggatag gtaggtatct
     4981 aatgcgccgc cgacgagatc gcgcagactt tcatttacgt catgcaaacg gacgaggtgg
     5041 tcgtaaatat cgcggaaaaa gatacggtct ttacggtcaa tgacctgaaa gtcgtcacgt
     5101 gccattttgt tgagtacttc acgctggggc agcagaatgc gccgcatggc aagcaggaca
     5161 cgtttgagtg tgaataatct ggcaagggtt tgtgaactgg ggcggtcaaa gacctggtct
     5221 tcgacccaat ctatctcttc atctattttt tcaattatgg gcatgtaatt catcacaatg
     5281 gcgtcaatga tcttgtagag caggtggtcg gcgccatctt gcgaatagcg cgggtcacgc
     5341 tgactggtat tccaggtttc attaatggaa gtgatggggt tatcgtggtg ggtgatgaca
     5401 tagtttcgcc ccaggaagat atccaactca tcgatctccg tatcccaggg ttcggtggct
     5461 tttgtcagat gcatatagtt tagaacgata tagaggtagt ctccccaatc atcgatcttg
     5521 ggtgcgtggg tttgttgcag ggcatcgtca atggctagtt ggtggaatcc aaaaccatta
     5581 aggatgggta atgcactttc ttcgggttcg gcctcaaaat ccacccaaag cagtccgcgg
     5641 cggtctcgca agaggcgggg aaactcttca gtagggatgt tggtgcgaac aggttttccg
     5701 ggcgaaaaaa atagcgaacg aatcatattt tgcctccaaa agcatggaaa attgatattt
     5761 tttgaggaaa gtatcaattt ttacaatata ctaattgata ttatttactt ttctattgac
     5821 aaagttcaaa aaagttgtat aattttatta gaaattggac gtgacacctt gatttttata
     5881 aaagccgatt gattttgaca agcaaaagga gtttccatgg acattttatc ggagcgtatg
     5941 tctgagttga aacgcaaaag cattcaggaa gatttggatg ccattcacct tgaagaagaa
     6001 gccgtcaaag ggaaaaccct tttagataaa aaccttgcat ggattggaaa ttggatggtc
     6061 tcacgcggcg agaaactgcg caaacgctac aaccaatcct tgcacgatgc caattcgacg
     6121 caacttgtaa agaaggcagc ttatcatgct aaataacacc attgaaaaag ccacgaaact
     6181 ctatcacgaa tatccgcgta cattctggat cttaatcgtc attactttta ttgatcgtat
     6241 tggcggtgcg ttgctatttc cattttttgc gctctatctt accagcaaat ttggggtggg
     6301 catggccgat gtcggcgtgc tttttgcagc gttttccgtt tcaagctttg tcggttccgc
     6361 tgtcggcggg gcattgaccg accgatttgg ccgcaaggga atcatcattt ttggcttgat
     6421 cgcatcctcg tttagcacgg tggcaatggg tctggtcaca tcgtttgaat atttctttgt
     6481 gctggctttc ttcattggaa tattgaccga tgtagctggc cctgctcatc aagccatgat
     6541 cgcagatatt ctgcctgaag ataaacgggc tgatggatac ggcattttgc gcgttgcgtt
     6601 taacctctct gtggtgatcg gccctgccat tgggggactg cttgcggctc gttcttacct
     6661 tatgctgttc ctgtccgatg cggccatttc cttgctgaca gtgatcctga tcgccatttt
     6721 cctgcctgaa accaaacccg aagcacaccc tgatgcaccc aaagaaagca tgacgcgcac
     6781 cttcgccggg tatgggcagg ttttccgcaa tacagccttc atgctcttcc ttggagcggt
     6841 gttgttgcaa gtcttcactt acatgaatat gaacacatcg ctgggtgtgt tcctgcggaa
     6901 tgaatacggc atcaacgaat cggggtatgg acttttgctc agtatcaatg ctgccatggt
     6961 tgtgttgatg cagttcccaa ttacacgccg catcacaaac ttcccgccca tgttaatgat
     7021 ggcggctggg accttcctgt acgtgatcgg tttctccatg tacggatttg taaccactta
     7081 tgccatgttc gtggcagcca tggttataat taccttcggt gagatggtgg tctcaccagt
     7141 tgcgcaggcg cttgtagcgt ccttcgctcc cgaagaaatg cgcggacgtt acatggcagt
     7201 ctccggcttt tcatggggga taccctttgc agtaggcccc taccttgctg gtctgatcat
     7261 cgatggaccc tcaccgcaca tgctgtggta tgcagcaggt attgttggta cattgtcaac
     7321 agtcggtttt cttgtactgt atcgagtgaa atcaaaacaa acacaaaact tggaactcat
     7381 gcctgaagtt acggcatcct aaccctggag gaatcctcat gcgaccagcc cctacccgtt
     7441 gtccgatctg ccagtctgaa ttaactgttg cacgattgca ttgttcgtct tgtgatacga
     7501 ccatcgaagg gaatttcgct tcgggtcagt tttcaaacct gaccccggaa cagctggaat
     7561 tcgtcttcac cttcgtgcgc tgtgagggca agatcaaccg catggaacag gagatcgggc
     7621 tttcctaccc gaccatccgt aaccgattgc atgagatcat ccgtgccctg ggatacgagc
     7681 caggccgtga cgaacccgtg gaaattacac aggaaaaacg gaatagtatt cttgacgatc
     7741 tgcatgcagg taagatcacg gcagatgaag ccacccgtct actgcgtgga gaagaggagt
     7801 aggccatgtc caagacaatt tcagcagggc gctcacccag agttgtcata gagaatattg
     7861 aaggtgatct cagccttgtc ggctgggaag gcgatgatat tttgctgaag ggtgatgaag
     7921 atgaaatgca tatccgtcag gatgggaatc aagtcatcat ttcgtgtgat gatgaccttt
     7981 ccctgcgtgt gcccaaatcg gcatcggttt ccatcaaaaa gatcggcggc gatgccgcca
     8041 tgcgcggagt gatgggtgat attgaattga acgaaatcga aggcgatctt tccatccgtg
     8101 atgtgaattc ggtttcgatc ggtacgatcc actctgattt cagtctgcgc ggtgcaaagg
     8161 gaagcattgc catcaaaaaa gtgcttagtg acgtatccat ccgtgatgtg gatggcaacg
     8221 tatcccttga atcggttgcc gatgatctgg ccttgcgcga tgtgcgtgga aatgtgagcg
     8281 caaatgtggc tgaggacgta gtgctttacc ttgatccgaa acccggtaat gcctatgctg
     8341 tgactgcggg tgaagatatt ctactggtca tgcctcccag agccaatgca accttgaccc
     8401 tttctgccga tgagatcgac atagaatgga agggtgttga caatgatgac gatgccactt
     8461 cgcgtgtggt gaccttgggc gatggttctg ccttggtgac cttaagcgca ggcggcgata
     8521 ttcgtgtgac caatcagtcc aatgcgggtg attcggctga ggattttggt aattttgcag
     8581 gcatcggtat ggattggtcc gggtttggcg agcggatctc gcgccaggtg gaacaggcga
     8641 caaaacgggc cacccgtcag gctgatgaag ccattcgccg tgcggctgca aatgtagagc
     8701 gacgtgccaa ggtgaatgta aatgcgaagg tggggcgttg gaattgggat ctatctccaa
     8761 agggtgtgcc agtgacaccg ccttcacaag cttcggaaga agaacgcatg gcaattttga
     8821 agatgctcca ggaaaagaag atttcggcag aagatgctga gaaattgctt tctgcattgg
     8881 aaggagggga gtcctaatgt catcggaaga gcgcaggaag attttgcaga tggtggctga
     8941 tggaaagatc agcgctgaag aagccgctgg attaatgcgc gcactggacg aggacgcagg
     9001 tgaagaggat tctgccgggg agcagatcga agttcttcaa gctgaatcag gtttcggtcc
     9061 cgaacagacc gaggcccctg agtttgacga agtccgcagg cgggcattcc gttttgcgat
     9121 gatcccgctt tggatcgggg tcattgtcac tgtgctgagc gcatggggaa tgtattctgt
     9181 acagatgggt gctgggttga atttttggtt cttttgcctg acacttccgc ttttgttcgg
     9241 ggttctgtta atcgcgttgg gtgcgggtgg cagggcttcg cgctggatct atgtcaacgt
     9301 ggatcgttcg catcaaaatg agtggccgag gaagattacc ctggctttgc ccattccgct
     9361 tggtttagtg agttggttct tgaagaattt cggttctcga atcgatgggt tgaagaaaac
     9421 cacagtggat gatgtgctga tggcgatttc aatgacaaaa tcggtcaccg agccgttgat
     9481 cgttcatgtg aacgacgaca atagcggtga tcgtgtgcaa gtgttcatag gatgagaggt
     9541 taacatggca aacgcagaag aacgaatgaa gatattgaag atgattgaag aagggaagat
     9601 cagcgcagac gaaggttcga agctgcttgc agctttgagc gggccgcgaa aaggtgtgcc
     9661 cacaccgccg cgcccgccca gcgtggggaa ttcgactccg cgctggcttc gcatccgtgt
     9721 gactgatact cgcacgggcc gttccaaggc ttctgtgcag attccgcttg cattggtgga
     9781 tgccggtatg aagatcggcg cgcatttcgc tcccgaagtg gaaggcgtgg atatgtccaa
     9841 tgtgatggaa gccctgcgca tgggcgtaac cggcaagatc attgacgtga ccgatgagga
     9901 agacggcgaa cacgttgaga tctacgtgga ataacatctt tatattttta ttctgctttg
     9961 cgttgcatgg cggggtaaag tttgcaggtc aaaactgttt gtaacagcct ggggggaagc
    10021 ggctgctatc cgctataaac aaccttcttc ggagggttgt ttatattaac gcaagaggct
    10081 gccttcaaat tctgatgtaa attacgcgag tacatctttt tnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn natgtagatc acgggagatt tgaatgagat ttgcgatctt
    10261 tggtacaggt ggtgttggcg gatactttgg tggaaaactc gcccaggtcg gtgaggatgt
    10321 caccttcatt gcacgtggaa agaaccttga atccttgaaa aattcaggtt tgcgtgtgga
    10381 ttcgattctg ggggattttg ttctggaaaa ggtgcaagcc accgatttac cgcattcgct
    10441 caaaaatatt gatgtgattt tgcttgccac caaaacatgg caattggatt ctgccattgc
    10501 tgaaatgaaa cccatgttag gagagcagac atcgatcctg cctctgttga atggcatgga
    10561 acacatggat acactccgtg cggaatttgg tgggcatgtt cttggcggct tgtgcaggat
    10621 tagttcattt ttgatcgcac cagcccatgt gagtcatgtt gcggtgaatc cacacattga
    10681 atttaacgaa tggaaacagg aaaccagccc gcgggtgggt gaggttttgt cggcgttttc
    10741 aaagatcaaa gagatcacgg tagatacccc gcccgatatc gaattggcca tgtgggaaaa
    10801 atatcttttg atctgtgcct tcagcggcgt gggggcgata acccgtcagc ctgtgggagt
    10861 gtttcgctcc gatcctgaat cacgcgccat gttcattcgg tcgttgcacg aagtggttgc
    10921 cgtggcccgt gcgcgcggtg ttaacctaac cgaaaaatct gctgaaagtg ttttggctcg
    10981 cattgatcaa acccagccag atatgatggc ttcgatgcaa aaagatgtga tggagggccg
    11041 tccctccgaa ttggaggcgc agatcggtgc cctggtccgc atggcgaatg cggcaaatat
    11101 ccctgtgcca accaacgaat tcatttattc aaccctcctg ccgcttgaac aaaaagcccg
    11161 cagcaaagca acctgatttt ttaccattta ccaacctacc catttttcca gtacaaatcc
    11221 atccctttag gcgatacact ccagagatgc attcgctata ctgatgcgca aggagataac
    11281 catgagtaaa aatatttttg tcgaactttc aaacgcaatc gccgatgccg ccgaacaggc
    11341 tggcaaattt actgtgttag tggatgcgcg ccgacgtttt cctgccagcg gcattgtatt
    11401 tgccaaagac ctgattttga cggcagacca tgtggtggaa cgcgaagagg atattaacgt
    11461 gatccttgct gatggcacgg aagtaactgc acgcctcgta ggtcgggacc ctggaactga
    11521 tctggcagtt ttgaaattag actcagcctc agccactccc gctgaagttt ccaagacacc
    11581 cgcacgcgtg ggacaatttg ttttggcagt tggccgacct tcttccaacg gaatcgaatc
    11641 cagctttggc tcggttaatt cgcttggcgg tcccgtccgc actgggcgtg gaggtatgct
    11701 ggaaaaatac atcaagaccg atgtggtttc ctaccctggc ttttcaggcg gccccctggt
    11761 caatggggat ggtaccgtac tcggtatcaa cacctcaggc tttggaaact ctggcgcaat
    11821 taccatccct gcggatgtag catggaaaat cgccgaagcg ctggcgaagc atggcaagat
    11881 caaacgaggg tacctgggca tccgcagtca ggttgttgaa ctctctgcag atggaaagaa
    11941 agccctgaag cgtgaacagg aaagcggttt gttattggtt ggtcttgaag ccgatagccc
    12001 tgcaggcaaa ggcggtttga tcgtcggcga tatccttgtg gggattgcag gcgaacctgt
    12061 ggcgcatcaa gatgacctgt tcgcccgttt gagtggtgat gtggttggta aatccactcc
    12121 agtggatgtg ttgcgtggag gaaaattgga aacgctcaaa gtggagattg gagaaagata
    12181 attgatccgt gtcgcggtcg tttcgccgaa ttctgctttg cgggtcggtc tgcgtgaact
    12241 gcttggcagg cagcccgata ttcaggtgat cggtgaagcg gttgatcttg aaagcgtcca
    12301 tgaaatggag gccgaggtat ttgtgcttgc ctcggtctct tctggtcgtt tgttggagaa
    12361 cagcatcacc tctccgatcc tgtttttgac agacgacatt gagtctgtcc gtatggtgtt
    12421 ggggtcgaag gtgcgtgcct ggggtgtgct ttcactcgag gccacggaag atgaattggc
    12481 ggcggcggtg cgcgcagtgg gggaagggct ttgggttggg gcaccaggcc tggtcggaga
    12541 aatgatccgc ttttcggggc gcagggaatc tttgggagaa gagtctctca cagagcaatt
    12601 gactgcccgc gagaaggaag tcattcaatt aatggcgcag gggttggcga acaaacagat
    12661 cgcactttcg ttggggatca gtgaacatac tgtgaaattt catttgtcat cgctgtattc
    12721 gaaactggga atttccagcc gtaccgaagc catcaaacgg ggaatcgagt tgggcttgat
    12781 ctcgctttaa gaacaaacat cccgccagc
//
LOCUS       DAOQJC010000113        12750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2417_length_12750_cov_9.573060, whole genome shotgun sequence.
ACCESSION   DAOQJC010000113 DAOQJC010000000
VERSION     DAOQJC010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12750
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2417_length_12750_cov_9.573060"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1063
                     /locus_tag="PLE39_14900"
     CDS             143..1063
                     /locus_tag="PLE39_14900"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNE69668.1"
                     /translation="MKILFLFLDGIGLGENNPEINPFARANMPYLESLLKGRKLLRES
                     APVDSESAALFSINPRLGVNGAPQSATGQAVLLTGINIPAEIGYHYGPKPNPEVAQYL
                     DGKTLFAKTVQAGKTAALLNAYPPRYFEGIDSGKRLYSAIPLALTNAGLPLFTHEDYF
                     AGKALSADFTGKGWHEFLNFPEAPIFETEEAGKRLADLARQYDFSFFEYWASDYAGHK
                     QDMGSAVRQMERFDSVLKGLLEHWDSNDLVLLTSDHGNMEDLSTRKHTEADVPLLLFG
                     EKSIRDEFGKDIRDLTGIAPAIEKGLGIRN"
     gene            1153..2148
                     /locus_tag="PLE39_14905"
     CDS             1153..2148
                     /locus_tag="PLE39_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69669.1"
                     /translation="MLSPADLIHLPYTPDLTEGGIAYALRTLPNRFDHAEGRQYDQLR
                     RTVAGAAVELAFRRHLSQQGIPFEIASVNPFTNPDQYDVSLGGRRCDLQSFFISHRNQ
                     IAQMRSDPSLILNAPALVPSEHHVNDGRADNDLYLFAFLSGLIAASQDDLKKVIETNQ
                     PRYLIHLMPEAWRRPYQWTPLAPLVLKSESDEELIVEISGQDEGRGFVTRVLSLPPKV
                     RVTVDDSFYSVMAIHVRNIPSARIGIHSPAFKEAHVIAPLEWGNLWLYGMDITLTGWI
                     TREEFRRRAKLIAPNSRVFQYDRTKTKNLAVPVSSLNPLGELFIRVREWEAGRKA"
     gene            2196..2423
                     /locus_tag="PLE39_14910"
     CDS             2196..2423
                     /locus_tag="PLE39_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69670.1"
                     /translation="MTLVTYEGVVQKGSILLSEGVQLPENVKVYVIVTEEVISTKING
                     KKPVQIVSPRLAKRDDAARFKMTVTEEKPNA"
     gene            2416..2799
                     /locus_tag="PLE39_14915"
     CDS             2416..2799
                     /locus_tag="PLE39_14915"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HNE69671.1"
                     /translation="MLEYDSKNYDPPAPIAYVTLRNPQNGTVIPNVPLLVDTGADATL
                     LPLSAVRQLGVEIEVDSEFEVQVFDGETKLLRVARLDLLVFNKTFKGEYLLIDRPDGI
                     LGRNILNHIRLLLDGPQQEWGEYKK"
     gene            complement(2977..4239)
                     /locus_tag="PLE39_14920"
     CDS             complement(2977..4239)
                     /locus_tag="PLE39_14920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE69672.1"
                     /translation="MKKHSLFTVLLSLRGNPRGCVYFEPLWGIPYNLFAPYVSIYMIA
                     LGMSEKQIGLIVSISWGFQIFLALISGVVTDKLGRRFTTLVSDILSWTIPSIIWAVSQ
                     NFWYFLIAGVVNSIWRISHTSWSCLLVEDAEQDQLVDIYTWIYIANLFVGFIAPLAGV
                     LIGVFSFVPTMRGLYVFAAVTFTLKAVLTYLWTEETAHGKVRMQETKDQSVLHVFGEY
                     KDVLRDLVRAPQTLYTAGIMLVMSITSLINGSFWSILVTEKLQIATENLAIFPFVKSA
                     IMLVFYFTVMPAIGRMHFKMPMVVGFLGYVASQVVLVNAPSGGYGFLIFSVFLEACSV
                     AAVSPLIDQMIVLTVDAKERARILSILSVGVILVTSPFGWIAGTLSTLDKDLPFLLNI
                     SLFVIGMVLAYFAGQFSQKKTVMAEAAG"
     gene            4309..5304
                     /locus_tag="PLE39_14925"
     CDS             4309..5304
                     /locus_tag="PLE39_14925"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69673.1"
                     /translation="MSSITIPARTETHTNLRPLNVLRDLPAVADLIETCFSSTMDSEG
                     RRYVQDMRRAGNDNSFVKWANRAIETTSLPLSGYIWEENGKVVGNASLVPFKHNKQRI
                     YLIANIAVHPDYRRRGIARALTQRALQHAHEKEINNIWLHVRDDNPGAIELYTKLGFV
                     ERARRTSWIASTDPHATSLQTNFIITKRNPRDWPIQHEWLTRLYPEPLAWHRNWSFSP
                     LKPGFWNWLYLAFIDVNIRQWAAAKKETDPQQPAPLEATLAWIPQGHGDALFAAIGER
                     SNPDALTALLLEARCELYHSHPSITLEFPSSQFDTAIQAAGFKAQRTLIWMQATL"
     gene            5339..5713
                     /locus_tag="PLE39_14930"
     CDS             5339..5713
                     /locus_tag="PLE39_14930"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNE69674.1"
                     /translation="MTKFFFRWAINAVALYVAVLVVPGIDLRGSWTGILWLALIIGLL
                     NALVRPLLKFLTCPLIILTLGLFTIVINTVMLLLTSKIAQSFGIGLTVDGFWTAVLGS
                     LVISIVSIVMSVIFRDELKGKS"
     gene            complement(5797..7449)
                     /locus_tag="PLE39_14935"
     CDS             complement(5797..7449)
                     /locus_tag="PLE39_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69675.1"
                     /translation="MFQLLNHRMLFAWMLSVITSYFVVGFFGNYYPSFVGVLVLAVIA
                     QTLLGVLIDRLFAKAERLYSSNKGDLFLALALFMVLTVFAAAMLQMANQFPSLFDAKF
                     VLLEQDQTFPFIIAGVLTIPLLALAGQFMGQSRFSAFVDSIATGVLVASFFFAVYFIF
                     SFIFNQPVFDTDDIFFDSDGWLWRTRFTTDAYEDYYWRSLHPFVLLVIRPLVFLVSLF
                     LKGDRLSAGLLLMPLAGAACVFMAWYFVKKVTGNSLYAVLIAALLGGTTGHLIFGFLI
                     ETYIFIAAVAMLSIILLLRDAPLFTLIITGLVSFGITLSNIIQPTIALVFVRRDLKQW
                     IKYGLIVGALAFPLTLLNNVIYPDSQPYFFDPSSYGTEGRNTFEMHPARAVAIGRVMF
                     LNSIVAPDPLILKRELPFLKVWIVEVSRKLYPQEANPMRSSEYETQFGTAMVVFWMAL
                     AALGGFGFLKNIKKQDNRFAYAFIFITLFSFVLHQKFGKELFLYATNWTYAIVLFLAL
                     GWREFMGRRWFQAVMLVFIALMLVNNSRLILTMLSTSALHIR"
     gene            complement(7449..8930)
                     /locus_tag="PLE39_14940"
     CDS             complement(7449..8930)
                     /locus_tag="PLE39_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69676.1"
                     /translation="MNQPLRLFLTTLTALAVAFAFSFFFLNLSGRSLAEQGLLIGFLA
                     ASLGWLIWNAPSVLSEWKFAMPKLGLRENLPGLLLALTFFAVYTSIGLQLNTLSVDTT
                     DNFLDADNSSWITRIADPAGSTLEMRGPHPFAYFIFRPFGWLLNLFTGSSALSATLLN
                     TFTGALCVFLVWLFVKRQIQDAVYALLIASLLGLSTAHIFFGSVVETYIFSAAALIGF
                     LLVLQKGSSSLPASVGVSVLTFGITLTNFVQNFILFAAAQIQAGTKNFKTLVLNIFRF
                     TAFTLALGILLSILHAAWFPSSRLFFLPSDAQAEEEFAFSVFQEPSWKVIGRVFLLVR
                     TVFLYAVIAPQPYVFGEEVGGKFPRFNFFRISPETFSYSSYDGVGNVLVLAWAVLLLV
                     SGFFFLRNLVGTRKVDLSAAFILCVLFNFVLHFNYGYEIFLYSPDWAYALILFVALSL
                     APLAKNRFFQGGLAVFLILLAYNQFQFFDFIFKTIALFFDKGA"
     gene            complement(8967..11024)
                     /locus_tag="PLE39_14945"
     CDS             complement(8967..11024)
                     /locus_tag="PLE39_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69677.1"
                     /translation="MKLFQFLLRVGGSLAVALIVSFSSAVIFFNYSGYENLSDRIFLV
                     VVPTLAIGYLFYESFPKYWPWLLQRQPAVLVTFGLLALLAAAVVVLPAAVSNVYYLGL
                     VSLALALFILMLPAAPAVERIRTSHSMLHYMLGFVLNLAVSYGVVGFMGGVFSYRFDV
                     IVFSILITLVGGVIGYYLVRRASRSMRDGFLRNPLNFFLVISLPAFFAAMLFLSLQFP
                     SMFSLAYIQIPLARFGMFISSAVVAGAWSVALLEQVELRGWYAAFKQSKAYAFINENL
                     PGLYAAASFFGINLIIARALNHPTFSINSIVFEADAGPWMSILGFPEGHDVNRAVHPL
                     VLITLRPLVGLIQIFTADKWFLGPIIAVAAMNGLCIFMAWLFAKRATQKDSYAFVFSI
                     ILGVTAAHLLFGSLTETYVFGMTSLILFFVLVQADEKRFSVLVPAGILVFGVTVTNIA
                     QGMIALFFKKFGFWKLVYYGIIVLTISIALTAFVSVLYPGNQTFFWVPADLAFEGRFS
                     KPIYAGPMDRVVEKFGAVGRAIFFHGVVAPTPIEDFTRKTTDPLIDFKTHDYHEHRYA
                     WYNGAAYLPVALWLGLFAGSVLLFFKNLRTSPHIPLMLGLLGSIGFTYFLHMNYGTEL
                     FLYSPYWTYLLIFFLMLAYVDFAGKRWFEAVLTVFAVTLMVNNAWFIFIILRGLAPYL
                     AAG"
     gene            complement(11097..11273)
                     /locus_tag="PLE39_14950"
     CDS             complement(11097..11273)
                     /locus_tag="PLE39_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69678.1"
                     /translation="MSNNEPKTTTIAETENFIAWRAEEPDGETTYHVELNNVTIHFFQ
                     EEWVEFLELVRSLK"
     gene            complement(11362..12576)
                     /locus_tag="PLE39_14955"
     CDS             complement(11362..12576)
                     /locus_tag="PLE39_14955"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNE69679.1"
                     /translation="MTEQQIPSSPVWGTNTKLVVALTVVVIVGALLVKFQFILTPLLI
                     ALVLAYLFHPLSSFLQRKLHLSWGASVGIVYLVVIILLLGLLTLGGVSLVQQAQSLVS
                     VVQTAIRSLPAFIDRISDKIYTFGPFKIDFRAFDLTNLGDQILGMIQPLLSRTGTLIS
                     TVAGGAANFLGWTLFVVLVSYFVLAESGGFRNRMISVDLPGYTHDFERLSRELGRTWN
                     AFLRGQIIIFFIAIIVYTIVLSVLGVHYALSLALMAGLARFVPYVGPAVNWGLMALVS
                     YFQVYKLFGLSPVYYALLVLLIAVIIDQIFDNVVTPRILSDSLKVHPAAVLVATIVAA
                     NLFGLLGVVVAAPILATAAILWKYIMRKMLDLDPWPEEEQTQPTPPPGSRILVSIRRF
                     FRTLSLNRSKGK"
BASE COUNT         3664 a         3398 c         3144 g         2544 t
ORIGIN      
        1 acgaaaatcg gcatggtttt tgtatcagaa agtagggggg aagtaattgg taattatcca
       61 aacacatagc acatctgatt ctcaaccttc aaccttcaac cttcaacctt caaccttcaa
      121 ccttcaacct tcaaccttta ttatgaaaat cctcttcctc ttccttgatg gcatcgggct
      181 cggcgaaaac aaccctgaga tcaatccctt tgcacgcgca aacatgccgt atcttgagtc
      241 actgctcaag gggcggaaac tgctccgtga atctgcacca gttgacagtg agtcggctgc
      301 gctgttttcc atcaacccca ggctgggcgt gaacggcgct ccacaatcgg caacggggca
      361 ggccgttctg ctgacaggca tcaacatccc tgctgagatc gggtatcact acggacccaa
      421 acccaacccc gaggtggcgc aatacctcga tggcaaaacg ctttttgcaa aaaccgtgca
      481 ggcaggcaaa accgccgcgt tattgaatgc atatccgccg cgttattttg agggaattga
      541 ttcgggcaag cggttgtatt ccgccattcc gctcgcgttg accaatgcag gtctgccgct
      601 gttcacgcac gaagattatt ttgcaggcaa agcgctttcg gcagatttca caggaaaggg
      661 ctggcatgaa tttttgaact tccccgaagc gccgatcttc gaaaccgaag aagcagggaa
      721 aagactcgcc gaccttgccc gccagtatga tttctctttt ttcgagtatt gggcaagcga
      781 ttacgcaggc cacaagcagg atatgggctc ggctgtacgc cagatggaaa gattcgacag
      841 tgtattgaag ggcctgctcg aacattggga cagcaacgac ctcgtcctgc tcacatctga
      901 ccatggcaac atggaagacc tctcgacccg caagcacacc gaagccgatg ttccgctttt
      961 attattcggg gaaaaatcca tccgagacga attcggaaag gacatccgcg accttacagg
     1021 aattgcccca gcaattgaaa agggattggg cattaggaat tagggtttta ctttttcatc
     1081 attcatcatt catccttcat cattcatcat tcatccttca tcgttcatca ttcatcattc
     1141 atcctttccc ccatgctctc ccccgctgac ctcatccacc tcccatacac gcccgatctc
     1201 acggagggtg gaattgcgta tgcactgcgg actctgccca accgtttcga tcacgccgaa
     1261 ggcaggcagt acgatcaact ccgccgcacg gtggcaggcg cagcagtgga attggcgttt
     1321 cggcgccatc tttcgcagca gggaattcca ttcgagatcg ccagcgtaaa tccattcacc
     1381 aaccccgatc aatatgacgt ttcgctgggc gggcggcgct gcgaccttca atcctttttc
     1441 atttcacatc gcaaccagat cgcgcaaatg cggagtgacc cgtcgttgat tctgaatgcc
     1501 cccgcgctgg tgccttccga gcatcacgtc aacgatgggc gcgcggataa tgacctgtat
     1561 ctgtttgcct tcctctcagg cctgatcgcc gcttcgcaag atgacctgaa aaaggtgatc
     1621 gaaaccaatc agccgcgcta tttgatccac ctcatgcccg aagcatggcg gcggccctat
     1681 caatggactc cgctggcgcc gttggtcttg aaatctgaat cagatgagga attgatcgtc
     1741 gagatcagcg gacaggatga aggtcgagga tttgtgacgc gggttctcag tctgcccccc
     1801 aaagtgcgtg tgaccgtgga tgattcgttt tattcggtca tggcgatcca tgtcagaaac
     1861 atcccttcag cgcggatcgg gattcacagc ccagctttca aagaggcgca tgtcattgca
     1921 ccgttggagt ggggcaacct gtggctctac ggcatggata tcacactgac aggctggatc
     1981 acccgtgaag aattccgccg ccgcgcaaag ttgatcgcac cgaattcacg cgtgtttcaa
     2041 tatgaccgca ccaaaacaaa aaatctcgct gtgcctgttt cgagtttgaa tccgctggga
     2101 gaacttttta tccgcgtgcg ggagtgggag gcagggcgga aggcgtgaaa gcggcagcag
     2161 cccacccatg ctataatttt cttcagaggt gccccatgac tcttgtaact tatgaaggtg
     2221 ttgtccaaaa aggcagtatt cttttaagcg aaggcgttca actgcccgaa aatgtaaagg
     2281 tctatgtgat cgttacggaa gaggtcattt ccacaaagat caacgggaag aagcctgttc
     2341 aaatcgtctc accgcgtttg gcaaaacgtg acgatgcggc tcgtttcaaa atgaccgtca
     2401 ctgaggaaaa gcccaatgct tgaatacgat tcgaaaaatt atgatccacc cgcgcctatt
     2461 gcctatgtaa cccttcgaaa tcctcaaaat ggaactgtaa tacccaatgt ccccttgttg
     2521 gttgatacgg gcgcggacgc caccttgtta cccctgagtg cagtcaggca acttggtgtg
     2581 gaaatcgaag tggattctga atttgaagtt caggtatttg atggggaaac aaagttatta
     2641 agagttgcaa gactcgatct gcttgttttc aacaagactt tcaagggaga atatcttttg
     2701 attgatcgcc ccgatgggat tttagggagg aatattttga atcatatccg tttgctgctt
     2761 gacggacctc aacaggaatg gggcgaatac aaaaagtaat cgagaaccac acctgtttcg
     2821 agtttgaatc tgctggggga actttttgtc cgcgtgcggg agtgggaggc agggcggaag
     2881 ggatgaaagc ggcagcctgc tcagcctgat gcaccctgtc catatggaca ggcgacccga
     2941 tttcaataca caactacaat tttcacatac attttcctaa cctgccgctt ccgccatcac
     3001 ggttttcttc tgtgagaact gcccggcgaa atacgccagc accatgccga tcacaaaaag
     3061 agagatgttc aaaaggaagg gcagatcctt atcaagcgtg gagagtgtac cggcgatcca
     3121 gccaaacgga gatgtcacca aaatgacccc cacagacaaa atggacagga tccgcgcgcg
     3181 ctccttcgca tccaccgtca gaacgatcat ctgatcgatc aacgggctga ccgccgccac
     3241 actgcatgcc tcaagaaaaa cgctgaagat caaaaaccca tatccgccgc tcggcgcgtt
     3301 caccagcacc acctggctgg ccacataccc cagaaacccc accaccatcg gcattttgaa
     3361 atgcatcctg ccgatggcag gcatcaccgt aaaataaaac accagcatga tggcagattt
     3421 tacaaagggg aatattgcca aattctcagt ggcgatctgc aatttctcag tcaccaaaat
     3481 agaccaaaaa ctgccgttga tcaacgaggt aatgctcatc accaacatga tccccgctgt
     3541 gtacagggtt tgcggcgcac gcaccaggtc acgcaaaaca tccttgtatt cgccaaagac
     3601 atgcagaacg ctttgatcct ttgtttcctg catgcgtacc tttccgtgcg ctgtttcctc
     3661 cgtccacaga taggtcaaca cagccttcag tgtaaaggtc accgctgcaa aaacatacaa
     3721 tccacgcatc gtcggcacga acgaaaatac cccgatcaac acccccgcca aaggcgcaat
     3781 gaaaccgacg aacaggttgg caatatagat ccaggtgtaa atatccacca actgatcctg
     3841 ctcggcatct tccaccaaaa ggcaactcca cgaagtatgt gagatccgcc agatgctgtt
     3901 gaccacaccc gcgatcaaaa aataccagaa attctgcgaa accgcccaga tgatcgaagg
     3961 gatcgtccac gacaaaatat ccgagaccag ggtcgtaaac cgccgcccca atttatcggt
     4021 caccaccccg ctgatcaacg ccagaaaaat ctgaaatccc caactgatgc tcacgatcaa
     4081 cccgatctgc ttttcagaca ttcccaacgc gatcatatag atcgaaacat acggagcaaa
     4141 taaattatac ggaatccccc acaaaggctc aaaataaacg caaccgcgcg ggtttccgcg
     4201 caggctcagc aacacagtga aaagggaatg ttttttcaat gaaggctcca gttcaaattt
     4261 ttttcgatta tataataccc tcggtcacaa agcgttataa tgccttcaat gtcttccatc
     4321 accattccag cgcggaccga aacacacacc aacctgcgtc cgctcaatgt gttacgcgac
     4381 cttcccgccg tggctgacct gatcgagacc tgtttctcca gcacgatgga tagtgaaggc
     4441 cgccgctacg tgcaagacat gcgccgcgcc ggaaacgaca actcctttgt caaatgggcc
     4501 aaccgtgcca tcgaaaccac atccctgccg ctgagcgggt atatatggga ggaaaacgga
     4561 aaagtcgtcg gcaacgccag cctggtccca ttcaaacaca acaaacaacg catctacctc
     4621 atcgccaaca tcgccgttca tcccgattat cgccgccgcg gaatcgccag ggcactcacc
     4681 cagcgcgccc tgcaacatgc ccacgaaaaa gagatcaaca acatctggct gcacgtccgc
     4741 gatgacaacc ccggcgcgat cgaactctac accaaactcg gatttgtaga acgcgcacgg
     4801 cgcacatcct ggatcgccag caccgaccct cacgcgacct ccctgcaaac gaacttcatc
     4861 atcacaaagc ggaatccacg cgactggccc atccaacacg aatggcttac ccgcctctac
     4921 cccgaaccgt tggcctggca ccgcaattgg tcattctctc ccctcaagcc cgggttctgg
     4981 aactggttgt acctggcctt cattgacgtg aacatacgtc aatgggccgc tgcaaaaaaa
     5041 gaaaccgacc cgcagcaacc cgctcccctc gaagccacgc tcgcctggat tccccagggc
     5101 cacggcgatg cccttttcgc agcgatcggc gaacggtcca accccgatgc gttgaccgct
     5161 ctcctgctcg aagcaaggtg cgagctttac cattcccatc ccagcatcac tctcgaattc
     5221 ccatccagcc aatttgacac cgccattcaa gccgcagggt tcaaagccca acgcacattg
     5281 atctggatgc aggcaacctt gtaacgcatt ttgcgtattt acaaacaaag gagttcaaat
     5341 gactaaattc ttttttcgct gggcgatcaa cgcggtcgct ttgtatgttg cagtactggt
     5401 cgtgccaggc attgacctgc gcggctcatg gacgggtatt ctctggctgg cgcttatcat
     5461 cggcttatta aacgcactgg tgcggccttt gttgaagttc ctcacctgtc cgctcatcat
     5521 cctcaccctc ggcctgttta ccatcgtcat caacacggtc atgctcctgc tcaccagcaa
     5581 aattgcgcaa agcttcggca tcggcctcac cgtggatggc ttctggaccg cggtactcgg
     5641 aagcctcgtc atcagcatcg tcagcatcgt aatgagtgtc atcttccgcg atgaattaaa
     5701 ggggaagtcg taagacagtc acaagaaata taaaaaaaaa gaggtttggc gcaaatgcca
     5761 aacctctttt tgattccagt ccgaagtgcg tcgcactcat ctaatatgca gagctgatgt
     5821 gctcaacatt gtcaggatca aacgcgagtt attcacaagc atcaaggcaa tgaaaaccaa
     5881 catcaccgcc tgaaaccagc gcctgcccat gaattcccgc caccccaacg caagaaaaag
     5941 gacgatcgca tacgtccaat tggtggcgta gaggaacaat tccttgccaa atttttggtg
     6001 aagcacaaaa ctgaacaggg tgatgaagat aaaagcatag gcaaaacggt tatcctgctt
     6061 tttgatgttc ttcaaaaaac caaacccgcc caacgccgcc agcgccatcc aaaagaccac
     6121 cattgccgtg ccaaattgcg tttcgtattc actgctgcgc atcgggttgg cttcctgcgg
     6181 atacaacttg cggctgacct caacgatcca cactttcaaa aacggcaatt cccgtttcaa
     6241 gatcagcggg tcgggggcca caatgctgtt caggaacatc acccgtccga tcgccaccgc
     6301 ccgggctgga tgcatctcaa aagtattacg cccttccgtg ccataggaag atggatcgaa
     6361 gaaatacggc tgcgaatcag ggtagatcac attgttgagc aaggtcagcg ggaaagccag
     6421 tgcgccaacg atcaggccgt atttgatcca ctgtttaaga tcacgccgca cgaaaacaag
     6481 agcaattgtc ggctggatga tattcgagag cgtaatgcca aatgaaacca gtcccgtgat
     6541 gatcaacgtg aacagaggcg catccctcaa cagcaaaatg atcgaaagca tcgccacagc
     6601 cgcaataaaa atataggtct cgatcaaaaa gccgaagatc aaatgtccag ttgtgccgcc
     6661 gagcagagcc gcgatcaaaa ccgcgtataa agaattgccc gtcaccttct ttacaaaata
     6721 ccaggccatg aacacacagg ccgcccccgc caaaggcatc aacaacaatc ccgccgaaag
     6781 ccgatcacct tttagaaaaa gtgaaaccaa aaacaccagc ggacgaatca ccaacaacac
     6841 gaacggatgc agtgaacgcc agtaataatc ttcataagca tctgtcgtga agcgtgtgcg
     6901 ccacaaccag ccgtctgaat caaagaaaat atcgtctgta tcaaaaacag gctggttgaa
     6961 aatgaacgag aaaataaaat agaccgcaaa aaagaaggag gcaaccagca cacccgttgc
     7021 gatcgaatcg acaaaagcag aaaaccgcga ctgccccatg aattgccctg ccaaagccaa
     7081 aagcggaatg gtcaacaccc cggcgatgat gaacggaaaa gtctggtcct gctcaagaag
     7141 gacgaacttc gcatcgaaca aactcggaaa ttggttcgcc atctgaagca tggccgccgc
     7201 gaaaacagtt aacaccatga aaagcgccag cgccaaaaac aggtcacctt tattggacga
     7261 atacaaccgc tccgccttgg cgaacaaacg gtcgatcagc actcccaaca gggtttgggc
     7321 aatgaccgcc agcacaagca cccctacaaa ggacgggtaa taattaccga aaaacccgac
     7381 cacaaaatac gaggtaatga cagacaacat ccacgcaaaa agcatgcgat gattaagcaa
     7441 ttggaacatc aggcaccttt gtcaaagaat aacgcaatgg ttttgaaaat aaaatcaaag
     7501 aactgaaact gattataagc aagcaaaatg agaaagaccg ccagaccacc ttgaaagaaa
     7561 cggttttttg ccaacggcgc aagggaaagc gccacgaaaa ggatcaatgc atacgcccaa
     7621 tcgggggagt acagaaagat ttcgtaccca taattgaaat gcaggacgaa attgaacaga
     7681 acgcacaaga taaacgcagc agagaggtca actttgcggg tgccgaccag attcctcaaa
     7741 aagaagaagc ctgaaaccaa aagcaggaca gcccaagcca gcacaagcac attccccacc
     7801 ccatcgtatg aactatagga aaaggtctct ggagaaatgc ggaaaaagtt aaaacgcgga
     7861 aacttccccc ccacctcctc gccgaacaca tacggctgcg gagcaatgac cgcatacaaa
     7921 aacaccgtac gcaccagtaa aaacacacgt ccgatcacct tccacgatgg ctcctgaaac
     7981 acagaaaagg caaactcctc ctccgcctgc gcatcggaag gcaggaaaaa caacctgctc
     8041 gaaggaaacc atgcggcatg gagaatgctg agcaaaatcc ccagcgcaag agtgaaggct
     8101 gtaaaacgaa agatgttcag caccagggtc ttgaaatttt tcgtgcctgc ctgtatctgg
     8161 gcagcggcaa acagaatgaa gttctgaacg aagttcgtca gtgtaatgcc gaaggtcagc
     8221 acgctcaccc caacagaagc aggcagggaa ctggatcctt tttgaaggac aaggagaaac
     8281 ccgatcagcg ccgcggcgga gaaaatataa gtctccacga ccgagccgaa gaaaatgtgc
     8341 gctgtactca gccccaataa agatgcaatc aacaaggcat acaccgcatc ttgaatttgg
     8401 cgcttcacga acagccagac caggaaaaca cacagcgcgc ctgtgaatgt gttcaacaag
     8461 gtggctgaga gggcagaact ccccgtgaag agattcaaca gccagccaaa cgggcggaaa
     8521 ataaaatacg caaacggatg tggtccgcgc atttcgaggg tggaacctgc cgggtcggca
     8581 atgcgtgtga tccaggaaga gttatcggcg tcgaggaagt tgtcggtcgt gtctacgctg
     8641 agggtgttga gctgcaggcc aatggatgtg tacaccgcaa aaaaggtcaa tgcaaggagg
     8701 agaccaggca ggttttcacg cagccccagc ttgggcatgg caaacttcca ttcactgaga
     8761 acagagggtg cattccaaat caaccacccc aacgatgcgg ccaaaaatcc aatcagcaat
     8821 ccttgctctg ccagagaacg acccgacaaa ttgaggaaga aaaagctgaa cgcaaaggca
     8881 accgccaatg cagttaaagt agtgaggaaa agcctgaggg gctgattcat tattttgaac
     8941 ggaatcaggc gcgaggtccg ctgtgattag cctgcagcga ggtagggggc aagtccacgc
     9001 aggatgatga agatgaacca ggcgttgttg accatcaaag tcaccgcaaa aacggtcagc
     9061 acggcttcga accagcgttt ccctgcaaaa tccacatagg ccaacataag gaagaaaatc
     9121 aacaggtagg tccaatatgg cgagtaaagg aagagttcgg ttccgtaatt catgtgcagg
     9181 aaataggtaa aaccgatact tcccagcagc cccaacatca gcggaatatg cggcgaggtg
     9241 cgcagatttt tgaagaagag caaaaccgaa cctgcgaaca aaccaagcca gagcgccacc
     9301 ggcaaatagg ccgcgccgtt gtaccaggca tagcggtgtt cgtgataatc gtgggtcttg
     9361 aaatcaatca gcggatcggt ggtcttgcgg gtgaaatctt caatgggtgt gggggcaaca
     9421 accccgtgga aaaagatggc acggccaaca gcgccgaact tttcaacgac acgatccatc
     9481 ggccccgcat aaatgggttt ggaaaaacgt ccttcaaagg ccagatctgc agggacccag
     9541 aagaaggttt ggttgcccgg gtacagcaca ctgacaaaag cggtcaatgc gatgctgatg
     9601 gtcagaacaa tgatgccgta ataaacgagt ttccaaaaac cgaatttttt gaagaagagt
     9661 gcgatcatgc cttgcgcaat gttggtgacc gtcaccccaa aaacgagaat ccccgcaggc
     9721 accagcacgg aaaacctttt ttcatcggcc tgcacgagga cgaagaacag gatcaatgag
     9781 gtcatgccaa agacataggt ttcagtcagc gagccaaaaa gcaaatgcgc ggcggtaaca
     9841 ccaagaatga tcgaaaaaac aaaggcgtag gaatcttttt gcgtggcacg ttttgcaaaa
     9901 agccaggcca tgaagatgca aagtccgttc attgcagcaa cggcaatgat ggggccgagg
     9961 aaccatttat cggcagtgaa tatttggatc aggcccacca gaggccgcag ggtgatcagc
    10021 accaaaggat gtacggcgcg attcacatcg tgtccctcgg gaaaaccgag aatggacatc
    10081 cacggacccg catcggcttc gaaaacaatg ctgttgatgc tgaaggtggg gtgattcaat
    10141 gcgcgggcaa tgatgaggtt gattccaaaa aacgatgcgg cggcatacag gccaggcagg
    10201 ttttcattga taaaggcgta agcctttgac tgcttgaagg ccgcatacca accgcgcagt
    10261 tccacctgtt ccagcagggc aacgctccat gcgcctgcca ccaccgcgct ggatataaac
    10321 atgccaaacc gcgccagcgg aatttgaatg tatgccagcg agaacatgga tggaaattgc
    10381 agcgacagga aaagcatggc cgcaaaaaaa gcaggcagcg aaataaccag gaaaaaatta
    10441 agcgggttac gcaaaaatcc atcacgcatg gaacgggatg cacggcggac gagataatag
    10501 ccgatgacac ctcctacaag cgtgatgagg atggaaaaaa cgatcacatc aaaccgataa
    10561 ctgaacacgc cacccatgaa accgaccacc ccgtaagaga cggcaaggtt caacacgaaa
    10621 ccgagcatgt aatgcagcat cgaatgcgag gtgcgaatcc gctccacagc gggtgcggca
    10681 ggcagcatga gaatgaacaa ggccaaagcc agtgaaacca gcccaagata atacacgttc
    10741 gaaaccgccg caggcaaaac aacaacagca gcagccagca atgccagcag gccaaaggtc
    10801 accagcacag caggttgtct ttgtagaagc cagggccagt attttggaaa tgattcgtag
    10861 aaaagatacc caatggcaag ggtgggtaca accaccaaaa aaatgcggtc tgagaggttt
    10921 tcgtagccag agtaattaaa aaagatcacc gccgaggaaa aactgacgat tagtgcaact
    10981 gccaaactcc cccccacccg aagcagaaac tggaataatt tcattcagtg gactccaaaa
    11041 aagggtaacg acctcagccg cataaaacaa aggctgaagt cgttacggca aagattctat
    11101 ttcaacgagc ggacgagttc gaggaattcg acccattctt cctgaaagaa gtggatggtc
    11161 acattattta attcaacgtg gtaggtggtt tcaccatcgg gttcttcagc gcgccaggcg
    11221 atgaaatttt cggtttcggc gatggtcgtt gttttgggtt cgttattgct catgatgttt
    11281 ctccttgggt ctattgaatg atgaatgagt ttcagcggtt ggcaggggcg cgcggccccg
    11341 cttgtcaatg ctacggggtg gttatttccc tttgctgcga ttcaggctga gggtccgaaa
    11401 gaagcggcgg atcgagacga ggattcttga cccgggaggc ggtgttggtt gagtctgttc
    11461 ctcttcgggc cagggatcca aatccagcat ttttcgcatg atgtacttcc acaaaatggc
    11521 ggcggtggcg aggatgggcg cggctacgac cacacccaac aaaccaaaca ggttggccgc
    11581 aacaatggtg gcaaccaaca cggccgcagg gtggactttt agcgagtcgg aaaggatgcg
    11641 cggggtgaca acgttatcaa agatctggtc aatgatgaca gcgatgagca gcaccaacag
    11701 cgcataatac actggtgaaa ggccgaacag tttgtaaacc tgaaagtatg aaacgagcgc
    11761 catcaatccc cagttgaccg cagggccgac atacggaaca aaacgggcca gccctgccat
    11821 gagtgccagg ctgagggcat aatgcacgcc cagcacgctt aatacaatgg tgtacacgat
    11881 gatggcaata aaaaagatga tgatttgtcc gcgcaggaag gcattccagg tgcggcccag
    11941 ttcgcgggag aggcgttcga aatcgtgggt gtagccgggc aggtccacac tgatcatgcg
    12001 gttgcgaaac ccgccgcttt cagccaacac aaagtaagaa acgaggacga cgaacagagt
    12061 ccagccaagg aagttggcgg cgccacccgc cactgtgctg atgagtgtgc ccgttcggct
    12121 cagcaagggt tgaatcatgc ccaggatctg gtcgccgagg ttggtgaggt caaaggcgcg
    12181 gaagtcaatt ttgaaggggc cgaaggtata gattttgtca gagatcctgt caatgaaagc
    12241 tgggagggat cgtatggcag tttgcaccac agaaaccagg ctttgtgcct gctgtacaag
    12301 gctcaccccg ccaagggtaa gcaaccccaa cagcaggatg atgaccacca gatatacaat
    12361 accgaccgaa gccccccagg aaaggtggag tttacgttga agaaaactgg aaagtgggtg
    12421 gaataaatag gccagcacca gggcgatcaa cagcggggtc aaaatgaact gaaacttgac
    12481 cagcaacgcg ccaacgatca caaccactgt gagggccacg acaagtttgg tatttgtgcc
    12541 ccataccggg gaagatggga tttgttgttc ggtcataagg ttattggttc cagtatgcgt
    12601 cgtttacggg ttcagttcga ttctatcaaa gatgggtgat tttacaacat ttcacaaggc
    12661 aggcggtatg gctttctcaa agtcgggagt acttgactaa ccgctaagag cgcaaagagc
    12721 gcgaagttta atggattttc ttggcgttct
//
LOCUS       DAOQJC010000114        12563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2477_length_12563_cov_10.178206, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000114 DAOQJC010000000
VERSION     DAOQJC010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12563
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2477_length_12563_cov_10.178206"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..430
                     /locus_tag="PLE39_14960"
     CDS             41..430
                     /locus_tag="PLE39_14960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE69680.1"
                     /translation="MSKGSVLIVEDNMDTYELVNFILQKDGFDTFLAVNGRDGVDAAI
                     KQKPDLIIMDLSMPEMDGWAATSLIKKNPTVKSTPVIVLTAHALPGDRQRAMDAGCDQ
                     YITKPMDLGELVEVIHYWIKKAKSSQP"
     gene            455..1750
                     /locus_tag="PLE39_14965"
     CDS             455..1750
                     /locus_tag="PLE39_14965"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNE69681.1"
                     /translation="MKQICVVGVGYVGLVTAACFADLGNRVTALDVNEKRVENLKKGI
                     MPIYEPGLDELVKRNVNAGRITFTTSYKEALQGAEYAFIAVGTPSGEKGEADLQYVAA
                     AAKSIAENMTAPIVIINKSTVPIGTGDWVADIVKGAQPKPMQFAVVSCPEFLREGSAI
                     GDFMNPHRTVIGSLDKDAANKVAHLHLPLRAPIVITDLRTAEMIKYASNAFLATKISF
                     INELADLCELVGADVKEVAAGMGYDARIGRHFLDAGLGWGGSCFPKDVEALAFMAKEK
                     GLNPRILNDVMEVNYDRRTTAVKHVEKMIGSELKGKTIGLLGLAFKPNTDDMRDAPSI
                     DIADALLKAGAKVRAYDPVAMDVARPILPAVDMFDEPYKMAKDCDALMVVTEWNEFKQ
                     LDLEKLKGLLKSPIIYDGRNIYDPRLMKEMGFTYRAIGR"
     assembly_gap    1817..1916
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2044..2946
                     /locus_tag="PLE39_14970"
     CDS             2044..2946
                     /locus_tag="PLE39_14970"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE69682.1"
                     /translation="MTNPPVCNYEGSDYQQTFWDQGGREYEDRAEAIALKRMLPTSGK
                     LMLELGAGAGRNTPRYAGFERIVLLDYSTTQLAQAQQRLGRSAQYIYVAADVYRLPFR
                     QHQFDAATMIRTLHHMADAPKALGQVRNVLRSNAVFILEFANKLNLKSILRYWLGRQD
                     WNPFSLEPVEFVKLNFDFHPKAVRNWLGGLGFSIEKTLTVSHFRLGFLKRVIPAKLLA
                     ALDGLFQPTGALWQFTPSVFLKAKVNENTQNAEIPLSVLGLFKCPECSGENLQEKNDH
                     LLCPNCNARWSVNDGIYDFREKMK"
     gene            2980..3723
                     /locus_tag="PLE39_14975"
     CDS             2980..3723
                     /locus_tag="PLE39_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69683.1"
                     /translation="MTTDPQKPLPHVQPFVERKTQPPRKNGVDTRGMLSVVTMLVSLA
                     ALTVSMLGAVKLVFDIFDDGLSGSKLEGLLVKLTVLGFAFLFGWAVGLVSIRAFGNLV
                     YPIIVKVYSWGCLIGTSVLYINVIQKLYFQKYDGLHFWAYLIMLLSGLFVLICLHLLV
                     EGHDLRPYAIPLLIISVLQLFVIVIRYVFDENPNGLMLFGDFTIFIVMISISALMLMH
                     IGILSPLRDQINNLFMRNRPSVKNGNGAG"
     gene            complement(3792..6998)
                     /locus_tag="PLE39_14980"
     CDS             complement(3792..6998)
                     /locus_tag="PLE39_14980"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNE69684.1"
                     /translation="MTLPNQSASTRKTIAVLGAQLSRSWGAEFMDGVLESARASDVNV
                     VCFVGGRPMAIASPQHGGHSYGLYDLIKPGQFDGILLSADLGHGASSEALGDFCRVFE
                     PTPVVSFAIPAEGVTTFSVDNEGGMRSMIRHLIEVHGYKRIAFIRGIQGHLEADQRYN
                     AYLEELKAHDIRFDEQLVVDGDYTNESGRAAARVLLDDRAVRVQAIVASNDRMAFGAI
                     EVLQQRGIQVPDSIAVTGFDDVNESRSMGVPLTTVNQSFFETGKKTFDALMKRMNGEK
                     LSDVNVIPTQLVVRWSCGCLPENVKRAVVLPREVSHTGRLENKRDAAIRALFGAAGIP
                     EDSPERVQYVDVFGRTWDMFLVSLREVEKSDTFLKMVQSMVELLQRQGYDSTTWHNVI
                     SIFRRYALGGITSNTTMLRAENLFQQARMLVGELSQRAQAFRRLQFEQQEEALSNFSF
                     SMAPAMTLEEIGDALSRHFPILGLNRWYLMFYSDFASPGSISSPTPESYRLLLQYDQN
                     KFQIPQEKSSIATGRLVPRGKTPEDHRYAAIVMPLALASNRFGFMWAEMGPTDWDVYM
                     RLKNLLSSALLRTMLVQQREQAQQEVERLLSEARERAVELARARDAAEKAAAQNAKLF
                     ESEQIRRRGAEALARSSRQLSSLTTIEKLPQQILEQLKQVLPYDRGILLMEDVNGVPR
                     IRAHHGMPANQDLSDFQIQLDGKDFYKTIARKGETLLVHNVEAVKDWRQPAWLPRDHS
                     WMGVPLYSKDKVIGLLMLSRMYSGFNEDDGLLATTFAMQAIVALENARLYDEVTGINQ
                     MMERMVSQRVEELNNAYNTLAKHDKNKSAFIQVAAHELRTPLTVIKGYLGMLSVDAVV
                     QANPVLAQAIEGVSQGTSRLHQVVSSMLDVARLDNQITNPHVEPVSLNLILRLIHKDY
                     VKDLETRHLNFELDEAIKDIPPLLADPELLKKALDHVVVNAIKFTPDGGTISISAIVF
                     EDARLGKQAEIRIKDTGIGIDPEHHKIIFEKLFQLGTVELHSSSRTNFKGGGAGLGLA
                     IASGIVKALQGNIWVESVGHDETNFPGSTFVIRLPLVK"
     gene            complement(7081..7404)
                     /locus_tag="PLE39_14985"
     CDS             complement(7081..7404)
                     /locus_tag="PLE39_14985"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNE69685.1"
                     /translation="MSTAPKSQILTLAEMASYQDAAVVSRQITKAEAGNVTLFAFDKD
                     QGLSEHTAPFDALVHILEGEALVTISGKPFELKAGDAIIMPANEPHALQAIQRFKMLL
                     TMIRA"
     gene            complement(7459..9498)
                     /locus_tag="PLE39_14990"
     CDS             complement(7459..9498)
                     /locus_tag="PLE39_14990"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HNE69686.1"
                     /translation="MIDNIDTEKRSGISAVAIGIVALLCCICVLVGGMVWYGYYAFTQ
                     AIPAISTFEPPTNDDPFFPVDPTEPVIEPELTRPPVDSINGETLQLLETTVVPPNEPK
                     ELACRMEGKCDIPDVMATTAAPRVVGDTDSFWVTDVGTNENTEVQATLRYVTPHVYFW
                     VQDGVSYDDGEMKALMDAFENKIYPTNREFFGSEWSPGIDGDEHIYILYARGLGSSIA
                     GYFSSADSVHPLVHEFSNAHEMFLFNADNTFLGEEFTYGVLAHEFQHMIHWNQDLNET
                     SWLNEGSSELAAFLNDYDPGGFDWLYITDPDMQLNDWPNDQDATTPHYGAGFLFMTYF
                     LDRFGEDATKALIKDPANGLDSVENALREIDATDPVTGQPISADAFFMDWAVTNFLLD
                     KSVGDGRYIYNNYPAANRASATETIYNCPQTPIKRDVHQYGVDYIAIECAGDHTISFT
                     GSTVTGLLPADPYSGDYAFWSNKGDESDMTLTREFDFTNVSGPIELSYQTWYDIETDW
                     DYLYLETSEDGVTWEIVTTPSGTDTDPSGNSYGWGYTGVTNGWIEEKVDLSQFAGKKV
                     FVRFEYVTDAAVNGEGLLLDDVKVEAAGYSSDFETDDGGWEAKGFVRVQNVLPQTFGL
                     SLILTSDSSVTMIPLNPDQTAEISISLQSGQKAYLVVSGTTRFTRELASYQIEIK"
     gene            complement(9679..12153)
                     /locus_tag="PLE39_14995"
     CDS             complement(9679..12153)
                     /locus_tag="PLE39_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE69687.1"
                     /translation="MMRFDRFTERAQEAAQRAAEIIQRYGHNQIDTEHILLALIEQPG
                     GVIPQILEKLSVSPEALTERLDATLRASPKANIFGGGAGQIFITPRVKRIIDLANEEA
                     NRLKDEYISTEHIFLAILTERNTPAARILESAGLTRDRVYDAIQDLRGGQRVTDPQAE
                     TRYRTLEKYSRDLTQLAREGKLDPVIGRDKEILRLIQILSRRTKNNPVLIGEAGVGKT
                     AIAEGLAQKIASNDVPEILSGKKVVALDLGAMIAGSRFRGEFEERLKAVMDEVQRAKG
                     DVILMIDELHTVVGAGAAQGAMDASNMLKPALARGELQCIGATTLDEFHKHIEKDAAL
                     ERRFAPIYVDEPSVDDTIKMLHGLRDRYEAHHKIHFSDDALTAAARLADRYVTDRHLP
                     DKAIDLMDEAAAKLRVALYSMPPDLKALKGEVDRLHAEEEQAGLERDYERAAQKKAER
                     LRLEQEYTVKRDKWEADHQLDEVVDVDDIASVVHQWTGIPVNQMLETESEKLLHMEAR
                     LHERIIGQDEAIRAISDAIRRARSGLKDPARPIGSFIFIGPSGVGKTELAKALAWFMF
                     DDEEALVRIDMSEYREQHTVSRLFGAPPGYVGYEEGGQLTEAVRRRPYRVVLFDEIEK
                     AHPEVWNALLQILDDGRLTDGQGKVVDFRNTVLIMTSNLGTEYVTKGGTLGFLTQKAD
                     VDERAMHDKIEKALKGAFRPEFLNRVDETIMFSPLSIEQMENIVVLQMKEVQDRLNEH
                     NITVQLTDAARGWLAKEGFDPAFGARPLRRAIQKYVESPLSVELLGGKFKDGAMVMVD
                     VDAENNKIIFHTEVAPAKASKQRVEA"
BASE COUNT         2950 a         3297 c         3269 g         2947 t
ORIGIN      
        1 gtacattgcc cgtaaacgcg agacgtagta tatttgcggc atgagcaaag gtagtgtttt
       61 gatcgttgaa gacaatatgg atacctatga attggtgaat ttcatcctgc aaaaggatgg
      121 gttcgatact tttctggcgg taaacgggcg tgacggtgtc gatgcggcga tcaaacaaaa
      181 acctgacttg atcatcatgg acctttccat gcctgaaatg gacggttggg cggcgaccag
      241 cctgatcaag aaaaacccca cagtgaaatc cactccagtc attgtattaa ccgcacatgc
      301 gctgcccggc gaccgccagc gcgcaatgga tgcgggctgc gaccagtaca tcaccaaacc
      361 gatggatctg ggcgaacttg tggaagtgat acattactgg atcaagaaag ccaaatcgtc
      421 acaaccctaa tctttttttc aaagaggaac aaacatgaaa cagatctgtg tcgtcggcgt
      481 cggatacgtc ggccttgtca ctgccgcttg ttttgccgat ctcggaaacc gagtcaccgc
      541 attggatgta aacgagaagc gcgttgaaaa tctcaaaaag ggcatcatgc ccatttatga
      601 gcccggtctc gatgaattgg taaaacgcaa tgtcaacgcg ggccgcatca ccttcaccac
      661 ttcctacaag gaagccctgc aaggcgccga atatgccttc attgcagtag gcaccccctc
      721 cggcgaaaaa ggcgaagcgg acctgcaata cgtagccgcc gcagccaaat ccatcgcgga
      781 aaatatgacc gcgccgatcg tcatcatcaa caaatccacg gttccgatcg ggaccggtga
      841 ctgggtggcc gacattgtga agggtgccca gcccaagccg atgcaatttg cggtcgtctc
      901 ctgccccgaa tttctgcgcg aagggtctgc cattggcgat tttatgaatc cgcaccgcac
      961 cgtgatcggc tcgctggata aggatgccgc caacaaggtg gcccacctgc atttgcccct
     1021 gcgcgctccc atcgtcatca ccgacctgcg caccgcagaa atgatcaagt acgccagcaa
     1081 tgccttcctt gccacaaaga tttccttcat caacgaactg gctgacctgt gcgaactggt
     1141 cggcgcggat gtgaaggaag tggccgccgg tatgggctac gatgcgcgca tcggccgtca
     1201 ctttttggat gcaggcctcg gctggggcgg ttcgtgtttc cccaaggacg tggaggcgct
     1261 cgccttcatg gccaaggaaa agggactcaa tccgcgcatt ctcaacgatg tgatggaagt
     1321 gaactacgac cgccgcacca ccgccgtcaa gcatgtggaa aagatgatcg gcagcgaatt
     1381 gaaaggcaag accatcggcc tgctcggttt ggcgttcaag cccaacaccg atgacatgcg
     1441 cgatgccccc tccatcgaca ttgccgacgc cctgctcaag gcgggcgcca aagtccgcgc
     1501 gtatgacccc gtggcaatgg acgtggcccg tcccattttg cccgctgtag atatgttcga
     1561 tgaaccctac aaaatggcaa aagactgcga tgcgctcatg gtcgtcaccg aatggaatga
     1621 attcaaacag cttgacctcg aaaaattgaa gggattgctc aaatccccca tcatctacga
     1681 tggacgcaac atctacgatc cacgtttgat gaaggaaatg ggcttcacct accgcgccat
     1741 tgggcgataa tttcagcaat ctgatcgttc gctctccgaa tagtcggatg gtctgcaaag
     1801 gccattcgac tattttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgat
     1921 cgttcgctct ccgaatagtc ggatggtctg caaaggccat tcgactattt tgcctatttg
     1981 accaaaagct aaaaaaacac tacactacaa aaaacaatca tcctccaacc acaagaccaa
     2041 aacatgacca atccccccgt ttgtaactac gaaggctccg attatcaaca gactttttgg
     2101 gaccagggcg ggcgcgagta tgaagaccgc gccgaagcca ttgccctgaa aagaatgctc
     2161 cccaccagtg gaaagctcat gctcgaactt ggcgcaggtg caggccgcaa cacaccgcgt
     2221 tatgcaggct tcgagcgcat tgtcctgctg gattactcca ccacccaact ggcacaggcc
     2281 cagcagcggc tgggacgctc ggcccaatac atttatgtcg ccgccgatgt gtaccgattg
     2341 ccattccgcc agcatcaatt cgatgccgcc accatgatcc gcaccctgca tcacatggca
     2401 gatgccccca aagcgctcgg tcaggtcagg aacgttctgc ggtcaaacgc cgtctttatc
     2461 cttgaatttg cgaacaaact caacctgaaa tcgatcctgc gctattggct gggtcggcag
     2521 gattggaacc cgttcagcct tgaacctgtg gaattcgtga agttgaattt cgatttccat
     2581 cccaaggcgg tccgcaattg gctgggcgga ctcggctttt ccatcgaaaa aacactgacc
     2641 gtctcacatt tccgcctcgg atttttgaag cgtgtgattc ctgccaaact tctggccgca
     2701 ctggatggtt tgttccagcc cacgggtgcg ctctggcaat tcacgcccag cgtcttccta
     2761 aaagccaaag tgaatgaaaa tacacaaaac gccgaaattc ctttgagtgt gttgggttta
     2821 ttcaaatgcc ccgaatgttc aggcgaaaac ctgcaggaga aaaatgatca cctgctctgc
     2881 ccaaactgca atgcgcgctg gtcagtgaac gacggcattt acgatttccg tgaaaaaatg
     2941 aaatagcacc acgaatcctg caaggccggg agatgcgcca tgacaacgga ccctcaaaaa
     3001 ccactgcccc acgttcaacc gttcgtggaa agaaagacac aacctccccg aaaaaacgga
     3061 gtggatacgc gcggcatgtt gagcgtggtc accatgctgg tcagcctcgc cgcgttgacc
     3121 gtttcgatgc tgggggcggt caaactggtc tttgatattt ttgatgatgg cctgagcggc
     3181 agtaaattgg aaggcctgct ggtaaaactg accgtgctgg ggtttgcgtt cttatttggc
     3241 tgggcagtgg ggctggtcag catccgtgct ttcgggaatc tggtttaccc gatcatcgtc
     3301 aaggtttatt cctggggatg cctcatcggc accagcgtgt tgtacataaa tgtcattcaa
     3361 aagctgtatt ttcagaaata tgatggactg catttctggg catatttgat catgctgctg
     3421 agcggcttgt ttgtgctgat ctgcctgcac ctgcttgtgg aaggccatga cctgcgccct
     3481 tacgccattc cactgctgat catcagcgtg ctgcaattat tcgtgatcgt gatccgctat
     3541 gtatttgatg aaaatccaaa cgggttgatg ttgttcggcg attttacgat cttcattgtg
     3601 atgatctcga tctcagcctt gatgcttatg catattggca tcctctcgcc gctgcgcgac
     3661 cagatcaaca acctgttcat gaggaaccgt ccctcggtga aaaacgggaa cggggcaggg
     3721 taaaccagcc aaaaataaaa tcacccgcga agtttgattt cgcgggtgat tttattttta
     3781 cagtgttagt gttacttcac caatggaagc cgaatgacga aggtgctgcc ggggaagttc
     3841 gtttcgtcat ggccgacact ttcaacccag atatttccct gcagggcttt cacaataccg
     3901 gaagcgatcg ccaaccccag cccggcgccg ccacccttga aattggtgcg gctggaggag
     3961 tgaagttcca cggtgccaag ctggaacagt ttttcaaaaa tgatcttgtg gtgctcggga
     4021 tcaatgccga tgcctgtgtc ttttatgcgg atctccgcct gtttgcccag gcgggcatcc
     4081 tcaaacacaa tggcagaaat ggagatcgtt ccgccatcgg gggtgaattt gatcgcgttg
     4141 acaacgacat ggtcaagcgc cttcttgagc aattcagggt cagccaaaag gggcggaata
     4201 tcttttatcg cttcatcaag ctcaaagtta agatgacgcg tttccaggtc cttcacataa
     4261 tccttgtgga tgagtcgcaa aatcaggttg aggctgaccg gttccacatg cggattcgtg
     4321 atctgattat ccagacgggc cacgtcaagc atactgctga cgacctgatg caggcggctt
     4381 gtgccttgtg aaacgccttc gatggcctgt gccaataccg ggtttgcctg tacaaccgca
     4441 tccacactta acatgccgag gtagcctttg atgaccgtga gcggtgtacg caattcatgt
     4501 gcggccacct gaatgaaggc ggacttgttc ttgtcatgtt tggccaacgt gttgtaggca
     4561 ttattcaact cttcaacacg ctgtgagacc atgcgttcca tcatttgatt gatgccagtc
     4621 acttcgtcat acagacgcgc attttccaat gccacgatgg cctgcatggc aaaggtggtt
     4681 gccagcaatc catcgtcttc attgaatccg ctgtacatgc ggctgagcat cagcaaaccg
     4741 atcaccttgt cttttgaata aagcggaaca cccatccacg aatggtcgcg tggcagccag
     4801 gcaggctggc gccagtcttt caccgcttcc acattgtgga caagcagggt ttcgcctttg
     4861 cgggcaatgg ttttgtagaa atccttgcca tcaagttgaa tttgaaaatc agagagatcc
     4921 tgatttgcgg gcatgccatg atgtgcgcga atacgcggca caccgttaac atcttccatg
     4981 agcaggatgc cgcggtcata gggcaggact tgtttcaact gctccaggat ctgttgggga
     5041 agtttttcga tggtggtaag cgatgacaat tgacgtgacg aacgcgccaa cgcttctgct
     5101 ccgcgacggc ggatctgctc ggactcgaac agttttgcat tttgcgccgc ggctttttcg
     5161 gcggcatcac gagcgcgggc aagttccact gcacgttcac gcgcctcact caacaagcgt
     5221 tccacttcct gttgggcctg ttcgcgctgc tgtacgagca tggtccgcaa aagggcgctt
     5281 gaaagcaggt tcttcaaccg catgtaaacg tcccaatcgg tgggacccat ctcagcccac
     5341 atgaaaccga agcgattact tgccaacgcc aaaggcatga caatggccgc gtaacgatgg
     5401 tcttcgggcg tctttccacg cggaaccagc cgtcccgtag cgatggagga tttttcctgt
     5461 gggatctgga atttattctg gtcgtattgc aaaagcaaac gataactttc gggggttgga
     5521 gaagagatcg agcccgggga ggcgaaatcg ctgtagaaca tcaaatacca gcggttcaaa
     5581 cccagaatcg ggaaatgccg cgaaagcgca tcaccgatct cttccagggt catggcggga
     5641 gccattgaaa agctgaaatt gcttaaggcc tcttcctgct gttcaaactg taaacggcgg
     5701 aaggcctgcg cccgctgcga caactcaccc accaacatgc gcgcctgctg aaagagattt
     5761 tcagcccgca gcatcgtggt attgcttgta attccaccga gtgcgtaccg tctgaaaatg
     5821 gaaatgacgt tgtgccaggt cgttgaatcg taaccctgac gctgcaacaa ttcgaccatg
     5881 gattgcacca ttttcaggaa tgtgtcgctc ttttccactt cacgcaagct gacgaggaac
     5941 atatcccagg tgcggccaaa cacatccaca tattgaaccc gttcggggct gtcttcgggg
     6001 atgcccgccg caccaaacaa agctcggatg gcggcatcac gcttgttttc gaggcgcccc
     6061 gtatgtgaaa cttctcttgg aagcaccacc gcccttttca cgttttcagg cagacaaccg
     6121 catgaccaac gcaccaccaa ttgggtgggg atgacgttta cgtcacttag tttttcgcca
     6181 ttcatgcgtt tcatcaacgc atcaaaggtc tttttccctg tttcaaaaaa tgactggttg
     6241 accgtggtca gcggcacccc catggacctc gattcattga catcatcgaa gcctgtcacg
     6301 gcgatgctat caggcacctg aatgccgcgt tgctgcaata cttcgatggc gccgaacgcc
     6361 atgcgatcat tggaggccac aatggcctgc acacggaccg cacggtcatc caaaagaacg
     6421 cgcgcagcgg cgcgtccact ttcgttggta tagtcgccat ccaccaccag ttgctcgtcg
     6481 aagcggatgt cgtgcgcttt taattcttcc aaataggcgt tataacgctg gtcggcttcc
     6541 aaatgcccct gaattccccg aataaaggcg atccgtttat aaccatgaac ttctattaaa
     6601 tggcggatca tggaacgcat tccgccttca ttatccactg aaaaagtggt gaccccttcg
     6661 gcagggatcg cgaacgaaac caccggggtg ggttcaaaca cgcgacaaaa atccccaaga
     6721 gcttcagaag aagcaccatg accaaggtca gccgaaagca aaataccgtc gaactgcccc
     6781 ggtttgatca aatcgtacaa cccgtaggaa tgcccgccat gttggggcga agcaatggcc
     6841 attggcctgc cccccacaaa acaaacgaca tttacatcgc tggcacgcgc cgactccagg
     6901 acgccatcca tgaactcagc gccccaacta cggcttaact gcgcgccgag gacggcgatt
     6961 gtttttcgtg tactggcaga ctggtttggg agtgtcattt tgagtggcaa aattatacgt
     7021 caggaaatat gaatcgtgtc tgtcaatttt gaaaagaaat gaatttgtca cacagtgctt
     7081 ttatgcgcgg atcattgtca acagcatttt aaaacgttga atggcctgta aggcgtgcgg
     7141 ttcattggca ggcatgatga tggcatctcc cgctttcaat tcgaacggct ttcctgaaat
     7201 ggtcacaagc gcttcccctt caaggatgtg aaccagggca tcaaacggcg ctgtgtgttc
     7261 gctcaagcct tgatctttat caaaggcaaa caaggtgaca ttgccagcct ccgctttggt
     7321 gatctggcgg ctgaccacgg cggcatcctg atacgatgcc atttccgcga gggtcaaaat
     7381 ttgtgatttg ggtgcagtgg acatgagtac tccaaaaaaa gcaggccaca cccgtaggtg
     7441 tgacctgccg attatttatt attttatttc gatctggtag gaggccagtt ctctcgtgaa
     7501 ccgtgtggtg cctgagacaa ccagatatgc cttttgcccg gactgcaagg agatggagat
     7561 ctcagcagtt tgatcggggt tgagcgggat catcgtcacg cttgaatcgc tggttaatat
     7621 cagagacagg ccaaaggttt ggggaagaac gttttggact cggacaaagc ccttcgcttc
     7681 ccagccgccg tcatccgttt caaaatcgga actgtagcct gctgcctcaa ctttgacatc
     7741 atccagcaat aggccttcac cattcaccgc ggcatctgtc acatattcaa agcggacaaa
     7801 caccttcttg ccagcgaatt gggaaaggtc cactttttct tcgatccagc cgttggttac
     7861 accggtgtag ccccagccat acgaattccc ggaggggtcg gtatcggtgc ccgagggtgt
     7921 ggtaacgatc tcccaggtga cgccatcttc cgaggtctcg aggtagaggt aatcccaatc
     7981 ggtttcgata tcgtaccagg tctggtagga aagctcgatg gggccgctga cattggtgaa
     8041 atcgaactca cgcgtcaggg tcatgtctga ttcatcgccc ttgttcgacc agaaggcgta
     8101 atcaccggaa taaggatcgg cgggcagcaa cccggtgacc gttgaacctg tgaacgaaat
     8161 ggtgtgatca ccggcacatt caatggcaat gtaatccacg ccatattgat gcacatcgcg
     8221 tttgatcggt gtctgcgggc agttgtagat cgtctcagtg gcagaggcgc ggtttgccgc
     8281 aggataattg ttgtaaatgt aacggccatc gcccaccgat ttatcaagca ggaagttggt
     8341 tacggcccaa tccatgaaga aggcatcggc gctgatgggc tgaccggtaa cagggtcggt
     8401 ggcgtcgatc tcacgcaggg cattctccac gctgtcgagg ccatttgccg ggtctttgat
     8461 gagtgccttg gtggcatcct cgccaaaacg atccaggaag taggtcatga acaaaaagcc
     8521 tgcgccataa tgcggggtag tggcatcctg atcgttgggc cagtcgttca attgcatatc
     8581 agggtcggtg atatacaacc agtcgaatcc accggggtcg taatcgttga ggaatgcggc
     8641 cagttccgaa gaaccctcgt tcaaccagga ggtctcattc aggtcttgat tccagtggat
     8701 catgtgttgg aattcatgcg ccagcacgcc ataggtaaat tcctcaccga ggaaggtgtt
     8761 atcggcatta aagaggaaca tctcatgagc gttggaaaat tcatgcacca acggatgcac
     8821 agaatcagcg gaggagaaat aacctgcaat ggaagaaccc aatccgcggg catagagaat
     8881 gtaaatgtgc tcatcaccgt caatgccagg cgaccattca ctgccgaaga attcgcggtt
     8941 ggtggggtag atcttgtttt cgaaggcatc catcaacgcc ttcatttcgc cgtcatcata
     9001 cgacacacca tcctgcaccc agaaatacac atgcggggtg acgtatcgca gggtggcctg
     9061 cacctcggtg ttttcattgg ttcccacatc agtgacccag aacgaatcgg tatcaccaac
     9121 aacacgcgga gcagcggtag tggccatcac atcggggatg tcgcatttcc cttccatgcg
     9181 gcaggccaat tccttcggct cattcggcgg gacaactgtt gtttccaaaa gctgcagtgt
     9241 ctccccgttg atggaatcca ctggcgggcg ggtaagctct ggttcaatga cgggttcagt
     9301 ggggtccacc gggaaaaatg gatcgtcatt cgttggcggt tcaaacgtgg aaatggcggg
     9361 tatggcctgg gtaaaggcgt aatatccata ccaaaccata ccgccaacca gcacgcagat
     9421 acagcacagt aaagcaacaa tgccgatcgc aacggctgat attcctgatc tcttctctgt
     9481 atcaatatta tcaatcatgg tcatcctctg gttggttaag atgccccatg ataccgtgag
     9541 tgcgtgaaaa agggattagg aaattggagg gggaaggaaa taaaaggatg aaggatgaag
     9601 gatgaaggat gaaggatgaa aaaaagacga aagaagcatt tgctttcttt cgtcttttta
     9661 ctttctactt tctactcttt acgcctcaac ccgctgtttg gatgctttcg caggagctac
     9721 ttcggtgtga aagatgatct tgttgttttc agcatccaca tccaccatca ccatggcgcc
     9781 atctttgaac ttgccgccga gcagttccac cgaaagcgga ctctcgacat atttctggat
     9841 cgcgcgtcgc agcggacggg cgccaaaggc aggatcaaag ccttccttgg caagccagcc
     9901 gcgcgcggca tcggtcaact ggacagtgat gttgtgctcg ttcaggcgat cctgcacttc
     9961 tttcatttgc aggacaacga tattttccat ctgctcaatc gagagcggcg agaacatgat
    10021 ggtctcatcc acacggttga ggaactcggg gcggaatgcg cctttgagcg ccttttcgat
    10081 cttatcgtgc atggcgcgtt cgtccacatc ggctttttgg gtgaggaagc ccaaagtgcc
    10141 acccttggtc acgtattcag tgcccaggtt cgaggtcatg attaagaccg tgttgcggaa
    10201 atccacaact ttgccctgac catccgtcaa gcggccatca tccaaaattt gaagcaaggc
    10261 attccatact tcagggtgcg ccttttcgat ctcatcgaag aggacgacac gatacgggcg
    10321 gcgacggacg gcctctgtga gttgaccgcc ttcttcataa ccgacatatc cgggaggcgc
    10381 gccgaacaaa cggctgaccg tatgctgttc gcgatattca gacatatcaa tgcgaacaag
    10441 cgcttcctca tcatcgaaca tgaaccaggc aagggccttg gcaagctcgg tcttccccac
    10501 accagaagga ccgatgaaga tgaacgatcc gatcgggcga gcgggatctt tcaacccgga
    10561 acgcgcgcgg cgaatggcat cagaaatagc gcggatggct tcgtcttgac cgatgatgcg
    10621 ttcatgcaaa cgcgcttcca tatgtaacaa tttttctgat tcggtttcaa gcatctgatt
    10681 cacagggatg cccgtccact gatgcacgac ggaggcgatg tcgtccacat ccaccacttc
    10741 atcaagttgg tgatcggctt cccatttatc gcgcttcact gtgtattcct gttcgaggcg
    10801 cagacgttcg gctttctttt gagcggcgcg ttcgtaatcg cgttcgaggc cagcttgttc
    10861 ctcttctgcg tgcagacgat ccacttcacc tttcaaggct ttgagatcgg gcggcatgga
    10921 atagagcgcc acacgcaatt tcgcggcagc ctcatccatc aggtcaatcg ctttatcggg
    10981 caggtgacgg tcggtcacat agcggtctgc caaacgcgca gcggctgtga gggcgtcatc
    11041 tgaaaaatgg atcttgtggt gcgcttcgta acggtcgcgc aagccgtgca acatcttgat
    11101 cgtatcgtca acgcttggct cgtccacata gatcggggcg aaacggcgtt cgagtgcggc
    11161 atctttttcg atgtgtttgt ggaattcatc caacgtggtc gcgccgatgc attgaagttc
    11221 gccgcgggcc aatgcgggct tgagcatgtt gctcgcatcc atcgcgccct gggctgctcc
    11281 tgcaccgacg accgtatgca gctcgtcgat catcaggatc acatcgcctt tggcacgctg
    11341 tacttcgtcc atcacggctt tgaggcgttc ttcaaattca ccgcggaagc gtgaccccgc
    11401 gatcattgca ccgagatcaa gcgcaacaac cttcttgccc gaaagaattt caggcacatc
    11461 gttgctggcg attttctgtg caaggccttc tgcgatggcg gttttgccca cacccgcttc
    11521 accgatcaga acgggattgt tcttggtgcg gcgggacaga atctgaatga ggcgcaaaat
    11581 ttccttgtca cgcccaatga ccggatcaag tttgccttcg cgggcaagtt gggtcaggtc
    11641 acgtgaatat ttttccaggg tgcggtaacg cgtctcggct tgcggatcgg tgacgcgctg
    11701 tccgccgcgc aaatcctgaa tggcatcata aacacggtca cgggtcaggc cagccgattc
    11761 caaaatgcgg gcggcgggcg tgttgcgctc ggtgagaatg gcaaggaaga tgtgttcggt
    11821 ggagatgtac tcatctttca ggcggttggc ttcctcgttg gccagatcaa tgatccgttt
    11881 gacgcgaggc gtgataaaaa tctgccctgc cccaccgcca aagatattgg ccttcgggct
    11941 ggcacgcagg gtggcatcca accgctcggt caatgcctcg gggctgacgc tcaatttttc
    12001 aaggatctgg ggaatcaccc cgccgggctg ttcgatcaac gccagcagga tgtgttcggt
    12061 atcaatttga ttatggccgt agcgctgaat gatctccgcg gcgcgctggg cggcttcctg
    12121 agctctctct gtaaatcggt caaatctcat catgggatag ttcctccgtt ggctgacggg
    12181 taaaaagacg atagacaacg gacggtttag cggcagtcag tggtcaatgg tctgcgatca
    12241 gtcaatgggg gcattataac aaaagcagga tttgcagatt gtatataagg cgtaagaggt
    12301 gtgtaagatt tttgcccccg ttgggaacag tggagccaga cttttagata tggcagtagc
    12361 aaagtcgctt gacaaaaata gagacttgga acgcgaatag tgcgaatggg cgaatttcgc
    12421 aaattttctt ggatttattc gccccattcg taaattcgtg gcattcgtgt tgaggttttc
    12481 ctgactttgc tatagccatg gatttttcga cgcaattact cccatttgcg agagacctaa
    12541 tgtataatcc cgaccatcct tgt
//
LOCUS       DAOQJC010000115        12368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2538_length_12368_cov_9.742142, whole genome shotgun sequence.
ACCESSION   DAOQJC010000115 DAOQJC010000000
VERSION     DAOQJC010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12368
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2538_length_12368_cov_9.742142"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="PLE39_15000"
     CDS             complement(<1..1150)
                     /locus_tag="PLE39_15000"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNE69688.1"
                     /translation="MSGQINAFEMAQKQFDGVAKQLKLDPGVAEVLRWPMREYSFRIP
                     VRLDDGSLKVFQGFRVQHNDARGPNKGGIRFAANETMDTVRALATWMTWKCAVADIPL
                     GGGKGGIIVDPSTLTVNEKEEMCRGWVRAMWKNIGPRNDVPAPDVGTTPQMMGWMMDE
                     YSRLVGQYTPGVFTGKPLGGGGSLGRTEATGYGVIYTVREAMKHLNIDGKNSIAAIQG
                     FGNVSQYAAVGFVEMLGGQVACVSCWDRHDKKSYTYSKKGGIDPRFLLTIVDEYGTID
                     KKKAQDAGYTVEDGDAWITKEANVLIPAALEGQVTADTVGKMSKGIKIVAEGANGPCT
                     PEADEHFKKNGIFNIPDFLCNAGGVTTSYFEQVQNDMNFYWSKEEVLER"
     gene            complement(1375..2145)
                     /locus_tag="PLE39_15005"
     CDS             complement(1375..2145)
                     /locus_tag="PLE39_15005"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNE69689.1"
                     /translation="MSTLHTDLKSGAAILTLNRPSRANSFNFEMTTALMDALSTAEND
                     PQVRCMVITGGGKVFSAGQDITEMKQGGGVVSYREHLEKTYNPLILQIRRMGKPVIAA
                     VNGPCAGAAFGIALACDLRIAHSRAYFVVGFSGIALAPDSGVSLLLPKYIGLGRAQEY
                     FYSNKPITSHLAYQWGLVNQVGGFNFNALILQIAGRLAAGPREAFAAGKKAFNQAILP
                     NLEESLRYEGILQDEAGRSAEHREGVAAFLGKRLPKFD"
     gene            complement(2213..2575)
                     /locus_tag="PLE39_15010"
     CDS             complement(2213..2575)
                     /locus_tag="PLE39_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69690.1"
                     /translation="MDDRITIIEGPPPIFEHVNDGWALGLNESSTLSVPALTRLRTFN
                     GPALVQRCYNAWHNKSSIHLHYRSEAGLEETAPIIAARNVDTPDGHMLLLWVYLDGEN
                     VEYEVDAGDEDQQDDFPE"
     gene            2699..3316
                     /locus_tag="PLE39_15015"
     CDS             2699..3316
                     /locus_tag="PLE39_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69691.1"
                     /translation="MTPKIILEGTRLTFKTDIAFALNEHPRIVGPRKYKYHSPLISGE
                     WCAFTNFPWGRGLINFEPQEEAQAMETYETWVKLFELQRYYSWIVDRFHLSTRMYQLN
                     THHKDYDFRWLEERLLPLNFRLVLLTRSPESFEAARAERLKVSGNPSQYDDLNLFIEE
                     QKLIRRLASESILPKLEIDISDNNIPAAVEKIADWMEATGGLYMP"
     gene            complement(3319..4410)
                     /locus_tag="PLE39_15020"
     CDS             complement(3319..4410)
                     /locus_tag="PLE39_15020"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNE69692.1"
                     /translation="MYNNYLRPFLFRLDPERAHTLTLDAVRMAGNMRLSLWLLERMFK
                     APSKPVELFGLTFRNPVGLAAGYDKDGVAIRGLAALGFGHVEIGTVTPRPQPGNPSPR
                     VFRLLEDEAVINRMGFPSKGSEFMQLKLNPSLRPGWFERIYGFQPHNKNKFERSINKG
                     GAILGINLGKNKETPNEDAVYDYVSLVDVFSRYADYLTINISSPNTVGLRELQGRAAL
                     EKLLAHIHQQRLVSQTYHEKRLPILVKLAPDLTDAELDDAIEAILQAKMDGIIVTNTT
                     LAREGLKSVLRGETGGLSGSPLAVKSEAVLHRTLKQVNGAVPIVSAGGIMSPDDAKRR
                     LEMGAALVQVYTGLIYRGPGLVREIVKSL"
     gene            4545..5684
                     /locus_tag="PLE39_15025"
     CDS             4545..5684
                     /locus_tag="PLE39_15025"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNE69693.1"
                     /translation="MSEKNSSKTSQPIMVGRHYPELDGIRSIAILLVIFFHCRIIAAP
                     QNPAEKVYYALVESGWIGVDLFFVLSGFLITGILLDTFKQENYFRNFYLRRILRIFPL
                     YYAVLLLFTLLAAFGLLKFFEQKSAIAQVSYWLYLQNWLALLDLAPTKIISHFWSLAI
                     EEQFYLLWPALVLFAARRNSVGRLGILTIIMAFIVRVVFVHYEKLAYFFTFSRMDTLA
                     LGACVAVLLRQHGSLGRFRTPALLLAAFSAVTVVTVGVTQKGFYGHDPEVLLYGLLPL
                     GIFFASFLTIALTSNENGALRLFLRNEHLRMIGVISYGVYIFHWPIIVLVQPMWPLVE
                     SFWVNQLGFLALITVTSIFVAWFSYRFFEKPFLQLKDKIAPVQKA"
     gene            5727..6689
                     /locus_tag="PLE39_15030"
     CDS             5727..6689
                     /locus_tag="PLE39_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69694.1"
                     /translation="MKPDKLEQAMELILSGNKRKALPILSRLVQEEPRNESAWVCLYE
                     CVDDLPKKTYCLKKILAINPDNQHARAELERISRSNTAIISLEQPPVIVVRGQTQLPI
                     QKTSTIDIIRYQFMKNPIRMWMIVILIMFLILNTGAFIFRWYTLRTVAVDHPLMPPLR
                     TTQTPYPMNLTPEYLYYATQESLPMVQANQIQIIIPETAQPQVSPMQQSLDEYLTAIE
                     QENWEAAYDHTCTAIQQVIHSPKEMRDRVFFEHSTLPVSHEILPPIDRPNRLLFTLTG
                     TTWSSRTFEVSLEINTLKFCGMGVERGDLRYLLFPAGGPLNIAQ"
     gene            complement(7058..7669)
                     /gene="pyrE"
                     /locus_tag="PLE39_15035"
     CDS             complement(7058..7669)
                     /gene="pyrE"
                     /locus_tag="PLE39_15035"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNE69695.1"
                     /translation="MTELNSNLASLADGLLSAGCIKFGEFTLKSGLKSPIYIDLRQII
                     TYPSLLEQVGAAYLPLLKELKFDRLAGLPYAAIPIATAVSLQGNYPMIYPRKEVKAYG
                     TKAEIEGEYHAGETALVIDDLATTGGSKFEAIEKLTAVGLAVKDVVVLVDRQSGAKES
                     LEQAGYSMHAVLTIRQMLDHWESTGKVEKDKIEATRKFLEQNG"
     gene            complement(7687..8523)
                     /gene="pyrF"
                     /locus_tag="PLE39_15040"
     CDS             complement(7687..8523)
                     /gene="pyrF"
                     /locus_tag="PLE39_15040"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNE69696.1"
                     /translation="METFFTFLEKRVDDSSSLLCIGLDPHIPDLKEPTAESALDFCLT
                     LIKQTAPYAAAFKPNAAFFEVFGAEGWLALKQVIEAINAESNRLGSRIPIILDAKRGD
                     IASTADAYARSAFENLGADCITLNPYLGKDSIEPFIQNSEKGVFLLCKTSNAGSMDLQ
                     NLLVLPMGSDTPMPLYIYVAKLAQEWNEKNNIGLVVGATHPQIMEMIRAAVPNLWFLS
                     PGVGAQGGELQLALKAGLRPDGKGILLPVSRAVARAQNPAKAAAELRDEILNIKRELK
                     LR"
     gene            complement(8619..12248)
                     /locus_tag="PLE39_15045"
     CDS             complement(8619..12248)
                     /locus_tag="PLE39_15045"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNE69697.1"
                     /translation="MTVDKPTLNKTENTVVFTITVSNGGPDNATGVKVKDLLPAGLTY
                     SSHSPGETYNSGTGIWDVGTLTSGGVGSTDSLTITATVTASFLPITNKAEVSSWDAGQ
                     TDPNSTPGNNSTTEDDDASTIITTQNANLSLTKTVNTTTPIVGSNVVFTVNVQNTGPF
                     QATNVTVTDLLPGSLTYISHTGAGTYVPGTGLWTLPTPLGVGAGASLQITASVDVPGP
                     ITNTATISNSDQYDNNPANDQASITLNPDTSDLSLSIAVNNTTPDKNDNVVFTVTLNN
                     AGPTTATNVVVTDVFPSGLSNIIDDDGGVGAPYPYNSNTWTIPSLPSGASAVLNITAN
                     VITQGAKIYSAQVWSSDQLDPNSTPGNNSTTEDDDASISLIPEVSDLSLIKTVDNPIP
                     NNGDTVVFTITVNNNGPDIATNVLVADVLPVPFTYISDDDGGGAFPYNAGVWSIPSLA
                     NGASAILHITALTGPGTLNTNYAEIMTSDQIDPDSTPGNGLIAEDDQDGSPSADLSLS
                     MTVSNPSPPLGSDITFVLTVNNIGVGTANNVFVKDILPAGLEWQSDNGAGAYNSGTGL
                     WNVGSLTPSGVGSTATISIVAKVMSTGVKTNFAEIVTSSLLDPDSTVSNGSVTEDDDA
                     NAVVTPAAGMLVIISEVAWAGTVAEGDDEWIELYNPGTSDININGWKLKDNSSIISLT
                     GSIPAGGYYLIEDTSVAVSDVTHDLIFNMSLSNSGESLELYDGSNILIDTANKDGGSW
                     PAGDVDTKGTMERIVSKTDSDDAWVTNTGFIKNGKDRNGGDIWGTPKNPAPVSTPTPT
                     PSGGMSIVISEVAWAGTAANSSDEWVELYNPGASQIDITGWKLIDGGGTEIILNGKID
                     PGSFFLLEDNENTVSDIIASQLDGNLALTNEGETLQLLDGSNTIIDSANNDGGGWPAG
                     SNSTYGTMERIVSSPDGNNAWYTNAGIVKNGKDANGGDIWGTPKQGNSPIPTATATAT
                     PITQTPTRTLTPTKTVTPTRTVTRTPTAEPVGRPVINEFLARPGYDWNRDGFVDVYDE
                     FIEIKNIGIVEINLKGWKLDDEADSGSTPFTFPDLLLLPGDRVVFYGNETRILLSDGG
                     DTVRLLNASNKVYDAYTYPLAKFKDKSVCRMPDGNGSWYEDCIPTPNLTNSRQGSVPS
                     MPGGSNAESELCDFPDTMPEDFIYAECRGFGGNIWDTFFWDQFGWLGRMIFQPASSKW
                     EIIVE"
BASE COUNT         2982 a         3118 c         3088 g         3180 t
ORIGIN      
        1 ggcgttcgag cacttcttcc ttgctccagt agaagttcat atcgttctga acctgttcga
       61 agtaggaggt ggtaacgccg cccgcgttgc agaggaaatc ggggatgttg aaaatgccgt
      121 tcttcttgaa gtgttcatcg gcttcggggg tgcaggggcc gttcgcgcct tcagccacga
      181 tcttgatgcc cttcgacatc ttgccaacgg tatcagcagt gacctgtcct tcgagcgcgg
      241 cggggatcaa cacattggct tccttggtga tccaggcatc gccatcttca acggtgtaac
      301 cggcatcctg agccttcttc ttgtcaatgg tgccatactc atccacgatg gtgagcagga
      361 agcgcggatc aatgccgccc ttcttgctgt aggtgtagga cttcttgtcg tggcggtccc
      421 agcaggaaac gcaggcaacc tgaccaccga gcatttcaac aaaaccaaca gccgcatact
      481 gtgaaacgtt gccaaagccc tgaatggcgg cgatggaatt cttgccgtcg atattgaggt
      541 gtttcatggc ttcgcgcacg gtgtagatca cgccgtagcc ggtggcttcg gtgcggccga
      601 gagagccgcc gccgccgagc ggtttgccgg taaatacacc cggggtgtac tgaccgacga
      661 ggcgggaata ttcgtccatc atccagccca tcatttgagg agtggtaccc acatcgggag
      721 cgggcacgtc attgcgtggg ccgatgttct tccacatggc acgcacccaa ccgcggcaca
      781 tttcttcttt ttcattaacg gtaagtgtgg agggatcgac aataatgccg cccttaccgc
      841 cgccgagcgg aatatccgcc acagcgcatt tccaggtcat ccatgtggcg agcgcgcgca
      901 cggtatccat ggtctcattg gcagcgaagc gaatgccgcc tttgttcgga ccgcgggcat
      961 cattgtgctg aacgcggaag ccctggaaaa ccttgagcga accatcgtct aagcgcacag
     1021 ggatgcggaa ggaatattca cgcatcggcc agcgaagaac ctcggctacg ccgggatcca
     1081 gcttcaattg cttggcaact ccatcgaatt gcttctgcgc catttcaaag gcattgatct
     1141 gtcctgacat tttttgttct ccttgatgag aatttgagaa aatataagcg ggcaccccgg
     1201 acggggtacc cgctctaaac acgagttatg tcaatctgct gaaccataaa gttaatccat
     1261 taaatcacaa gtacccctaa actttacacg agaatcagaa tgacgtcaat atgacaaaaa
     1321 tccgcatatt tgcgctgatt aatgatgagt tttgactggt tttgaaggaa attcctaatc
     1381 gaacttcggg aggcgctttc ctaaaaatgc cgccactccc tcacggtgtt ccgccgaccg
     1441 gcctgcttca tcctgcaaaa tgccttcata acggagagac tcttccaaat tggggaggat
     1501 cgcctgattg aaggctttct tccccgccgc aaacgcctca cgcggacctg cggccagcct
     1561 gcccgcaatt tgcagaatca gcgcgttgaa gttaaaccca cccacctgat tgaccaatcc
     1621 ccactgatac gccagatggg atgtgatggg tttgttggaa taaaaatatt cctgtgcgcg
     1681 gcccagccca atatatttcg gcaaaaggag cgatacaccc gaatccggtg caagtgcaat
     1741 accgctgaac cccaccacga agtaagcgcg tgaatgagcg atccgcaggt cacaagccag
     1801 tgcaatacca aatgccgccc ctgcacacgg cccattgacc gcggcaatga caggcttgcc
     1861 catgcggcgg atctgcaaga tcaaagggtt ataggttttc tccaaatgct cgcggtatga
     1921 aaccacgccg ccgccctgtt tcatttcggt aatatcctga ccggcgctga acaccttccc
     1981 accgccggtg atgaccatgc agcgaacctg cgggtcgttc tctgcagttg acagggcatc
     2041 catcaaggca gtggtcattt cgaagttgaa ggaattggcg cggctggggc gattcagcgt
     2101 cagaatcgcc gcaccagatt tgaggtcagt atgaagcgtt gacattcatc ctcgattcaa
     2161 aaaaagcgac cgtcaccttg tgcaaggtga cggtcaatac tttgagagtc agttattcgg
     2221 gaaaatcatc ctgctggtct tcgtcgcctg catccacctc gtattccacg ttttcgccat
     2281 cgagatacac ccacagaaga agcatgtgcc catcgggggt atctacattg cgcgcggcga
     2341 tgatgggggc cgtttcttcg agaccggctt cgctgcgata atgcaggtga atggatgatt
     2401 tgttgtgcca ggcgttgtag caacgctgca caagtgcggg cccgttgaac gtgcgcagac
     2461 gggtcagcgc gggaacggaa agggtcgagc tttcgttcaa cccaagcgcc cagccatcat
     2521 tgacatgctc aaaaatgggt gggggtcctt cgatgatcgt aattctatcg tccataaatt
     2581 tacctttggt ttcacctgtt cgggaatata ccacaacaat gcagaggaat cgtttcagaa
     2641 tattattaga atttttctgt aaggttgttc tgtgaatata attacaaata tgagcaaaat
     2701 gacccctaaa atcatcctcg aaggcacccg cctgaccttt aaaaccgaca tcgcgtttgc
     2761 gctcaacgaa cacccacgca tcgtggggcc gcgcaaatac aaatatcatt cgccgctcat
     2821 ttccggtgag tggtgcgcct tcaccaattt tccgtggggc cgcggtttga tcaactttga
     2881 accgcaggag gaagcccagg ccatggaaac ctacgaaacc tgggtgaaac tttttgaatt
     2941 gcagcgctac tactcgtgga tcgtcgatcg tttccatctt tccacgcgta tgtatcaatt
     3001 gaacacccac cacaaagatt acgattttcg ctggcttgaa gaacgcctgc tgccgctgaa
     3061 tttccgcctg gtgctgctca cccgctctcc tgagtctttc gaagccgcac gcgccgaaag
     3121 actcaaggtt tcgggcaacc catcgcaata cgatgacttg aaccttttca tcgaagaaca
     3181 aaaattgatc cgcaggcttg ccagcgaatc aattctgcca aaacttgaaa tcgatatttc
     3241 agacaacaac atccccgccg ccgtggaaaa gatcgcagac tggatggaag caacaggcgg
     3301 gttgtacatg ccgtaggctt acaaagattt cacgatctcc cgcaccagcc ccggcccgcg
     3361 atagatcaac cctgtgtaca cctgcaccag cgccgccccc atttcaaggc ggcgttttgc
     3421 atcgtcggga gacatgattc cgcccgcgct cacaatggga actgcaccgt tcacctgttt
     3481 cagcgtgcga tgaagaaccg cttcgctctt aaccgcaagc ggactcccgc tcaagccgcc
     3541 tgtttctccc cgcagcacag actttaatcc ctcgcgggca agggttgtgt tggtaacgat
     3601 gatgccatcc atcttcgcct gcaaaatggc ttcgatggca tcatccagtt cggcatcggt
     3661 caggtccggt gcaagtttga caaggatggg caatcgcttt tcgtggtacg tttgcgaaac
     3721 cagccgctgt tgatgaatgt gggcaagtaa tttttccagt gcagcacgtc cctgcaattc
     3781 acgcagtccc accgtattgg gcgaactgat attaatggtc aggtagtcgg catatcttga
     3841 aaagacatcc accagtgaaa catagtcata cacggcatct tcattggggg tttccttgtt
     3901 cttgccgagg ttgatcccca gaatggcccc gcccttgtta atggaacgct cgaatttgtt
     3961 cttgttgtga ggctggaagc cataaatgcg ttcgaaccac cccgggcgca gggatgggtt
     4021 caacttaagc tgcatgaatt cagagccctt gctcggaaag cccatgcgat tgatgaccgc
     4081 ttcatcttcc aacaggcgaa aaaccctggg agacggattg ccgggttgag gtcgcggggt
     4141 gaccgttccg atttccacgt ggccaaatcc cagtgcagcg agtccgcgga tggcaacgcc
     4201 atccttgtca taacctgccg caaggccgac aggatttctg aaggtcaggc caaacaattc
     4261 aacaggtttg gacggagctt tgaacatccg ctccaacagc cagagcgaga gccgcatgtt
     4321 ccccgccatg cggacggcat ccaatgtcaa tgtgtgggcg cgttcggggt cgagacgaaa
     4381 aagaaacggg cgaagataat tgttatacat caagtccttt ttatgattac actgcctatg
     4441 ctacaatcaa caaagttcaa aattcatttt aacccattgc aggcgaacgg ctgcggtatt
     4501 aaggcatcgg atggcgtggg agtttgtgag cagcgaaaag cagaatgtcg gaaaagaatt
     4561 cttccaaaac atcccagccg atcatggtgg ggcggcatta cccggaactg gatgggatca
     4621 gaagcatcgc catcctgctg gtcatatttt ttcactgccg tatcatcgcc gccccccaga
     4681 accctgctga aaaagtctac tatgccctcg tcgaaagcgg atggatcggg gtggacctgt
     4741 tcttcgtgct ttccggcttt ctgatcacgg ggattttgct cgataccttc aaacaggaaa
     4801 actattttcg caatttctat ctaaggcgca ttctacgcat ttttccactt tactatgcgg
     4861 tactgctcct ttttaccctt ctggctgcat tcgggctgtt gaaattcttc gaacaaaaat
     4921 cagccattgc acaagtttcg tactggcttt atcttcaaaa ctggctggcc ctgcttgacc
     4981 ttgcgcccac gaagatcatc agccattttt ggtctttggc gatcgaggaa cagttctacc
     5041 tgctttggcc tgcactggtc ttgtttgcag ccaggcgaaa ttcagtgggc aggcttggca
     5101 ttcttacgat cattatggca ttcattgtac gggtggtctt cgtccattat gaaaagctgg
     5161 cgtacttctt cacgttttca cgcatggata cactcgcact gggcgcttgt gtggcggtcc
     5221 ttctccgtca gcatggctcg cttggacggt ttcgcacacc tgcccttctt ttagccgcct
     5281 tcagcgccgt gaccgtggtc actgtgggag tcacacaaaa gggattctac ggccacgacc
     5341 ccgaggtgtt attgtatggc ctgctcccgt tgggaatctt ttttgccagt tttcttacca
     5401 tcgcacttac ttccaacgaa aatggagcgc tgcgactctt ccttcgcaat gaacatctgc
     5461 gcatgatcgg ggttatcagt tatggtgtgt atattttcca ttggcccatc atcgtcctgg
     5521 tgcagccgat gtggccgctc gttgaaagtt tctgggtgaa ccaactggga ttccttgcgc
     5581 ttatcacggt cacttccatt tttgtggcct ggttcagcta tcgttttttc gaaaaacctt
     5641 ttttgcaatt gaaagacaag atcgccccgg ttcagaaagc gtagttattt ttatttttgt
     5701 acaaattttt agatgattga gggaatatga aaccagataa actcgaacaa gccatggaac
     5761 tgatcctttc tgggaacaag cggaaggctt tgcccatttt gagcaggctg gttcaagagg
     5821 aaccgcgcaa cgaatcggct tgggtatgcc tgtatgaatg tgtggatgac ctgccaaaga
     5881 aaacatactg cctgaaaaaa atactggcga tcaaccccga taatcaacac gcccgcgccg
     5941 aacttgagcg gatatcacgg tccaacacgg ccatcatttc attggaacag ccgccggtca
     6001 tcgtcgtacg ggggcagact caacttccca tccagaaaac ctccacaatt gacatcatcc
     6061 gatatcagtt tatgaagaat ccgatccgaa tgtggatgat cgtgattctg atcatgttcc
     6121 ttatcttgaa cacaggcgcg ttcattttcc gttggtacac gctcagaacg gtagccgtgg
     6181 accatccgct gatgccgccg ctgcgaacca cccaaactcc ctatccaatg aacctgacac
     6241 ccgagtacct gtattatgcc actcaggaat cactgccgat ggtacaggcc aatcaaattc
     6301 agatcattat ccctgaaaca gcccaacctc aagtttcacc gatgcagcaa tcattggatg
     6361 aatatctcac tgcgatcgaa caggaaaact gggaggccgc atatgaccat acgtgtactg
     6421 ccattcaaca ggtgatccac tctcccaaag aaatgcgcga ccgtgtattt tttgagcact
     6481 ccaccctgcc cgtcagccat gagattcttc cgccgataga ccgccccaac aggctgcttt
     6541 ttactctgac aggcacaacc tggagttcaa ggacctttga agtcagtctg gaaattaaca
     6601 ccttgaagtt ctgcggcatg ggggtcgaac gcggcgattt gcgttacctg ttattccccg
     6661 caggcggccc gctcaatatt gcacaataga cctgtttcaa aagtctgaaa gattaaccac
     6721 ggagatacag agaaaacaat taaaaactcc gtggttcaaa atggcatttc aaagacattc
     6781 cttgcaactt acgcaagaag tcaaacaaat ttgcctgctg acaggtgaca aaaaaagaga
     6841 gcgaatggtc tctgcaatta caccagagat ctttcgctct ctttcagttt cttgtggcgg
     6901 gtgctttatt agaactcttg cagaagtcca tttttttacc accaaggcac aaagtctcga
     6961 agactcaaag aaaaaagctt agtgacttag cgcctttgtg actttgtggt ctacaacact
     7021 ttacttatgc aagaggtcaa ttccataaaa acctgaatca gccgttttgt tccaaaaatt
     7081 ttctcgttgc ttcgatctta tctttttcca ctttgcccgt ggattcccaa tggtcgagca
     7141 tttgacggat ggttaacaca gcatgcatgg aatagcccgc ctgttccagt gattcttttg
     7201 cgccggattg tctgtccacc agcacaacca cgtctttcac ggccagccca actgcggtca
     7261 gtttttcgat ggcttcgaac ttgcttccgc cggtggtggc gagatcatca atgacgaggg
     7321 ccgtttcacc tgcgtgatat tcgccttcga tctcggcctt ggtgccgtag gccttcactt
     7381 ccttacgtgg atagatcatg gggtaattgc cctgcagaga aacagctgtt gcaatgggga
     7441 tggcggcata aggcaggcca gccagacggt cgaacttcaa ttcttttaac agcggcaggt
     7501 aagctgcacc gacctgttcc agcaaactgg ggtaggtgat gatctgacgc aggtcaatgt
     7561 agatgggaga tttgagcccg gatttaaggg tgaattctcc gaacttgatg cagcccgcag
     7621 agagcaggcc atcggcaagg gatgccaggt ttgaattgag ttctgtcatg gatgattcct
     7681 tttcggttat ctgagtttca attctctctt gatgttcagt atctcatcgc gtaattcggc
     7741 ggcggctttg gcggggtttt gtgcgcgggc cacggcacgc gaaacaggga gcaggatccc
     7801 cttgccatcg gggcgcagac cggctttcag ggcaagctgc aattcgccgc cctgtgcgcc
     7861 gacacccggt gaaaggaacc acaggttcgg cacggccgcg cggatcattt ccatgatctg
     7921 cggatgggtg gcacccacca ccaggccgat gttatttttt tcgttccact cttgtgcgag
     7981 tttggcgaca taaatataca ggggcatggg ggtatctgaa cccatcggca atacgagcag
     8041 attttgcagg tccattgaac ctgcgttgga tgttttgcaa agcaggaaga cacctttttc
     8101 agaattttga atgaagggtt cgatggagtc ttttccgaga tatgggttca aggtgatgca
     8161 atctgcgccg aggttctcaa aagcggacct ggcgtaggca tcggcggtgg aggcgatgtc
     8221 accccgcttc gcatcgagga tgatggggat gcgcgaaccc agtcggttcg attcggcatt
     8281 gatggcttcg atgacctgtt tgagggccag ccacccttcg gcgccgaaga cttcgaagaa
     8341 ggcggcgttg ggtttgaatg ctgcagcgta gggagctgtc tgcttgatca gagtcaggca
     8401 aaagtcgagg gctgattcgg cggtgggttc cttgaggtcg gggatgtggg ggtcgagtcc
     8461 gatgcagagc agggaggatg agtcgtcaac gcgtttttcg agaaaagtga agaaggtttc
     8521 catgagacga tagtatacag ggaaacaagc aaaacggtaa accatccatc tgtttaccgt
     8581 ttgttgtttt atttattctt ttatttttag gtttgtggtt actctacaat gatttcccat
     8641 ttgcttgatg cgggttggaa gatcatgcgc ccgagccagc cgaattgatc ccagaagaag
     8701 gtatcccaga tgtttccgcc gaagccgcgg cattcggcgt agatgaaatc ctcgggcatg
     8761 gtatcgggaa aatcacaaag ctcggattcg gcattggagc cgcccggcat ggaggggacg
     8821 gaaccctgac gcgagttggt gaggttgggc gtggggatgc agtcttcgta ccatgagccg
     8881 ttgccatcgg gcatgcggca gacggatttg tctttgaatt tggcaagcgg gtaggtgtag
     8941 gcgtcgtaca ctttgttgct ggcgttcagc aggcgcacgg tgtcgccgcc atcgctgagc
     9001 aggatgcggg tttcattgcc gtaaaagacg acccggtctc cgggcaggag gagcagatcg
     9061 gggaaggtga agggtgtgga gcctgaatcg gcttcgtcgt ccaacttcca gcctttgagg
     9121 ttgatctcta cgatgccgat gttcttgatt tcgatgaatt catcgtacac atcgacaaag
     9181 ccgtcgcggt tccaatcgta gccgggacgt gcaaggaatt cgttgatgac aggcctgccc
     9241 acgggttctg ctgtgggcgt gcgggtgacc gtgcgcgtgg gggtgacggt tttggtgggc
     9301 gtgagggtac gcgtgggggt ttgggtgatg ggcgtggccg tggcggtggc tgtggggata
     9361 ggagaatttc cctgttttgg cgtcccccaa atatctccgc catttgcatc tttaccattt
     9421 tttacaatgc ctgcatttgt ataccaagca ttatttccat ctggagatga aacaatgcgt
     9481 tccatggtgc cataagttga attactccca gcaggccagc ctccaccatc attattggca
     9541 gaatcaatga ttgtgtttga accgtccaac aattgtagag tttcgccttc attagttaaa
     9601 gccagattcc cgtcaagttg gcttgctata atatctgaaa ctgtgttttc gttatcttcc
     9661 agcaagaaaa agctgcccgg gtcaattttg ccattcaaaa taatttcagt gccaccgcca
     9721 tctatcaact tccagccggt aatatcaatc tgtgaggcac cggggttata caactccacc
     9781 cattcatcgc tactgtttgc agcagtaccc gcccatgcaa cttcgctaat gacaatagac
     9841 attccaccgg acggggtcgg tgtgggcgtg cttacaggtg cgggattctt gggagttccc
     9901 caaatatcgc caccgtttct gtctttaccg ttctttataa acccggtatt cgtcacccaa
     9961 gcgtcatcac tatctgtttt agaaacaatt ctttccattg ttccttttgt gtccacgtcg
    10021 ccagcaggcc aactaccacc atctttatta gcagtatcaa tcaagatgtt tgatccatca
    10081 tataattcca gagattctcc agaatttgat aatgacatat taaaaattaa gtcgtgtgta
    10141 acatctgaaa cagccactga tgtatcttct ataagatagt aacctcctgc tggtattgaa
    10201 ccagttaagg agattatgga tgaattatcc ttcaacttcc atccattaat attgatatca
    10261 gatgtgcccg gattatatag ttcgatccat tcatcatcac cttcggcaac ggttcccgcc
    10321 caagcaactt cgctgatgat gaccaacata ccagcagcag gggtaactac tgcattggca
    10381 tcatcatcct cggtaacaga cccattggaa accgtcgaat cggggtctaa tagactgctg
    10441 gtaactattt ctgcaaaatt tgtttttacg ccagtagaca ttacctttgc aacaatgctt
    10501 atggttgcag tagaaccgac cccgctcggt gtcaaactgc caacgttcca taaccctgtc
    10561 ccactgttat atgccccagc gccattgtct gattgccatt ccagccctgc tgggagaatg
    10621 tctttcacaa atacgttatt ggcagttcca actcctatat tattgaccgt aagtacaaaa
    10681 gttatgtcac tacctaatgg aggtgatgga ttactcaccg tcatggaaag gctcaaatcg
    10741 gcagatggcg atccatcttg atcatcctct gcaatcaacc catttcctgg ggttgaatca
    10801 gggtcaatct ggtcacttgt cattatctca gcataattgg tatttaaagt ccccggcccg
    10861 gtaagagcag taatatgcaa aatggcactt gccccgtttg caagagatgg aatactccag
    10921 acacccgcat tgtagggaaa agcaccgcca ccatcatcat cagaaatata agtaaatgga
    10981 acgggcaaca catctgctac taaaacatta gttgcaatat caggtccatt attattcact
    11041 gtgatggtga agactacagt gtcaccatta tttggaatgg gattatccac tgtctttatc
    11101 aaacttaaat cagagacttc gggaattaag gaaatactcg catcatcatc ttctgtagtg
    11161 gagttatttc ccggggtgga attgggatca agttgatcac tagaccaaac ttgagcagaa
    11221 tatatctttg ctccctgagt aattacattt gcagtgatat ttaatacagc actagccccg
    11281 cttggaaggg aaggaattgt ccaagtattt gaattatatg gataaggggc accaacacca
    11341 ccatcatcat cgatgatatt gcttaaccca gaaggaaata catcagtaac aaccacattc
    11401 gttgcagttg ttggtcctgc attatttagc gtgacagtaa agacaacatt gtcgttttta
    11461 tccggagttg tattattaac tgcaatgctt aaacttaggt cagatgtatc gggattcagt
    11521 gtaatactgg cctgatcgtt ggcagggttg ttatcatatt gatctgaatt ggatattgtt
    11581 gcagtattgg tgattgggcc gggtacgtct acagaagcgg tgatttgaag agaggcgcct
    11641 gctcccacac caagaggtgt aggtaatgtc cataaacccg ttccgggcac ataggttcca
    11701 gcaccagtat gagatatata agtgagacta ccgggtaaca aatcagtgac cgtcacatta
    11761 gtcgcttgaa aaggacctgt gttttgaaca ttaacagtaa aaactacatt gctgccaaca
    11821 ataggtgtgg ttgtattgac ggttttggtt agactcaagt ttgcattttg agtggtgatt
    11881 attgtactgg cgtcatcgtc ttcagttgtt gagttgtttc cgggggttga attagggtcg
    11941 gtttgcccgg catcccaaga cgacacttca gctttgttgg taataggcaa aaaagaagct
    12001 gtcactgtcg cggtaatagt taatgagtca gtggacccca ctccgccgga tgtcaatgtt
    12061 ccaacatccc agattcctgt accactatta taggtttctc ccgggctgtg tgatgaatat
    12121 gtcagccccg caggcaataa atctttcact tttacgccag ttgcgttatc aggtccgcca
    12181 tttgatactg tgattgtgaa aactacagta ttttccgtct tgttgagcgt gggtttatca
    12241 actgtcatcg acaacgccag attcgagctc gtcgcccgca acaacggcgc ggcgctgaca
    12301 gcatctccct gcgtggcagg cgcagaaaca aaagccagca ggctcaccag cacggcaatg
    12361 aaaacaaa
//
LOCUS       DAOQJC010000116        12342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2550_length_12342_cov_8.667209, whole genome shotgun sequence.
ACCESSION   DAOQJC010000116 DAOQJC010000000
VERSION     DAOQJC010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12342
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2550_length_12342_cov_8.667209"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PLE39_15050"
     CDS             <1..498
                     /locus_tag="PLE39_15050"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE69698.1"
                     /translation="VAVGLGTDGEKENNNLDMFEELKTASLLAKLSALDAAALDAWST
                     CRMGTVTGARAIGLDSEIGSIEIGKQADLIAVRTDTPRLTPLLSGTAGNLHHNIVHAV
                     RGGDVDMTMVAGNILVDGGQLLTADLRQLIDEANTAVPPLFKRRAEWLATHKSVNELV
                     KHKDD"
     gene            567..872
                     /locus_tag="PLE39_15055"
     CDS             567..872
                     /locus_tag="PLE39_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69699.1"
                     /translation="MISMILGTLTVIALLLVVGALIHLKWTETISERTISRRIKAEYP
                     AEVQAQVYQTYQHMKVKELEGLFLKILDDANGDVLKVQKLASIAESVSWRAFLENHW"
     gene            954..1214
                     /locus_tag="PLE39_15060"
     CDS             954..1214
                     /locus_tag="PLE39_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69700.1"
                     /translation="MATLEQEEIALLKGRISRLEARLEFLYKHLNVTFVEDTRLSDDP
                     KVIAALKANNMLEAIKLYRAATNTSLEAAKAGVEEMRARLGI"
     gene            1387..5352
                     /locus_tag="PLE39_15065"
     CDS             1387..5352
                     /locus_tag="PLE39_15065"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE69701.1"
                     /translation="MPNIAAFVRKVYFPLVSLLCLIGVIGTGALSWRTSQDQDQRQQA
                     SFEFGSDQAERVATEVNAEMNLQMTIAETIAGEISDGTLAYTEITGRLRDELDAHPNF
                     FGMGVAFLPYVYKSDLKLFAPYYFKNEFGNFTRAQVETDYDYSDRATQDNEWFYSALE
                     DGAYWYASEYDITAQAELVEYITPFYRTNPQTGQQEAVGVVWVDHSVETMKNFIRSID
                     IGEDGYTTLIESAGYIVVHPKAEYINKTLYEVAELVGDTELSAAADRAISGEDFYLQR
                     KPTVNLNSTAWTFFKPLIAEGWSIAVTISDNVFEAPPEELLRTQIWLSIMIMTALISL
                     ALLIFRVDEGAVRNLWIVVGWSTFVLLSGVMWMWQIVNTNPSNITASKNILVSTSVVN
                     ESLKSVDETATINGRPIALRIPTGVLVQNIQIQPNEAVISGYLWQKYPKELPPNFMEH
                     LEHEFIQGVEFPDDTGATAFEESYRFQRADSEIVGWFFTTTLHQAYNTAQYPLDQANI
                     QIRISPQYLDQNIVLVPDFESYDFVAPSSLPGLSPQINIPDWNIQRSFFSYQLQQFNT
                     NFGSQTHIQRNNLPTLTFNISASRNLLSPIIAFCITIIVVAGLMFGSLIVKLDSAYSA
                     LSNAAALFFVVAITHVGLRGSIAAGGTVYLEYFFIVLYIFILGLALDGLMYYNESAIP
                     IISYGENLIPKLCFGPGLAITFLAITIFVFYPRTVAADPLQEYLSINALQATEEPAAS
                     DLTPTASPVTDSGSAANNLTATPIRPSTSPAATTVPVILPEDPITLRYPFGLEPPTLD
                     PSLVSLTSDQDQVEQLFIGLTRLESGTNTPLPNLAASWTTSEDGLTWTFHLRNDIPWV
                     QYNAATNSVSQVTDFNGTPRFVNAYDVVYGIHHALTSENTSAALLYEIKNAEQPDDDL
                     GVKALDEWTLEITLNRPAPYFASIVAYDVSYPVPGWAIEEWDKEWIAPENIVTNGPFV
                     LASWEKGKGITLLKNPLWPEADKIQIERVEEILVQDGEIALKLYRDNQIDTLGGLRSN
                     ILQEVKSDPTLANELSSHSNLCVRYFGFTNTKPPFDDPRVRRAFSAAVNRQEIVEMLK
                     VGNIPAFTFAPPGIFGAVTDPGVGQGFDADLAKQLFQEYLDERGFTIADFNSAYPLVM
                     GQLNGLNNSASDIAIKNWNDILGVTVTTKTVETAEFQSSIAKDNPIKDAFSMFQWAWC
                     ADYPDQNDFLHVLFNSETGTNLVRRNCADETCTAILPNDEFDNLTLMASQINDPEERQ
                     RLYAQAEHILVVEEAALAPIYYGASTSLTKPWLTRNFPNMGAPDFMNWIIDILAQKTT
                     P"
     gene            5371..7431
                     /locus_tag="PLE39_15070"
     CDS             5371..7431
                     /locus_tag="PLE39_15070"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNE69702.1"
                     /translation="MDKSLTPFFNPRGVVVVGASTSPEKLGYGVARNLIESKYRGAIH
                     FVSQKKGELFGHPLLLSLNEVPDPVDLAILIVPTQSTPQTIEDCARRGIKAAIIVSSG
                     FREVGAEGAALEAQCVELAHKHGVRLLGPNCIGTLDTHLPLDTTFLQPPMPTQGGIGF
                     ISHSGAFAAAVIDWAREQGFGFSQIVSLGNQADVNETDVLPAVANDEHTKVIALYMES
                     VSNGVKFVQAASEVTRHKPVIALKVGRFEAGQKAAASHTGALAGSEAAFDAAFAKAGV
                     LRADTAEQMFDWAQALENCPLPRGKRMAILTNAGGPGVIAADSLETNGLLLSQLTEST
                     LKALTSSLPPSASVHNPVDMLASASPDAYATCLNLLLQDETVDGVLVILPPPPMYKTE
                     EVAKKIIRVIGNFEKPVVVALMGSTLVENARKEFHAASVPNYPFPERAASALGALAKR
                     AEFFTTESQRHRELKTPRLGASVVNLSADELVSAYGIPTTPIKLARNENEAAAIADEL
                     GFPVVMKIASPDILHKSDVGGVLLNLTTPQTLLSAYTQMMERIKSAMPAAKIDGVHLQ
                     RQIPEGQEVIIGMVRDPLFGPLMMFGSGGIEVEGLKDVAFALAPLNQAEARKMIRETW
                     AGRKLKGFRSISAVDEESVIDVLIKLSQLAMEQETVSEIEINPLRVLPKGAVAVDVRV
                     KLRA"
     gene            7428..8225
                     /locus_tag="PLE39_15075"
     CDS             7428..8225
                     /locus_tag="PLE39_15075"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNE69703.1"
                     /translation="MIQLSNLTYYPIKACRGFDVAESQVERMGLANDRRMMVVTPSGK
                     FLTQREYPRLALVTPTVKNGMVTLSAPNFDSLQFGIQSTGTPTPVEVWKSKDVSAIDQ
                     GEESAQWLSDWLGVSVRLVHVADGFKRKLNPEYAVHADDHTGFADGYPILIISEESLQ
                     DLNSKLDSAVPMNRFRPNIVVKGCEPFAEETWKRIRIGGVEMALVKPCARCVVTTIDK
                     DTLAKSKEPLKTLSSYRMQELGAIFGVNVIPLNEGVVKVGMNVEVLE"
     gene            8262..8519
                     /locus_tag="PLE39_15080"
     CDS             8262..8519
                     /locus_tag="PLE39_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69704.1"
                     /translation="MEKTIIDILGWAGTILYLVAYGLISAKKVEGDSWLYQGLNIVAG
                     TFLIINTFYLRAYPSAGLNIAWVGIAIATLGRKAWTKASHD"
     gene            8512..9291
                     /locus_tag="PLE39_15085"
     CDS             8512..9291
                     /locus_tag="PLE39_15085"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNE69705.1"
                     /translation="MTSISRRDFLKLGGAALLGISAGKFIPPQEDEPNFTAPLIWSGS
                     RKFKRMAFTYDDCYLLNKMQDLEELLNEFPDFKVTFFPVGSKLLNLEEQDSGIWKRLV
                     EKGHEIGYHTFDHVNLGVMSPATALMDYDKWHAALNQVLGMEYTVRFVRPPYDIISYT
                     MDVLCQERGLVAAMFSIGGGGEPDIVTRAIQKGKGGDIVQMHIRTQDYKSSVQVFPWL
                     KENSWELVTMSKLYDDYLREQINPEGCELDAGSSLTRVCVE"
     gene            complement(9513..10496)
                     /locus_tag="PLE39_15090"
     CDS             complement(9513..10496)
                     /locus_tag="PLE39_15090"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HNE69706.1"
                     /translation="MKTIPRLNFAHLPTPIEELPHLTARLGGPRILVKRDDQTGLAFG
                     GNKTRKLEFLVAEAQEQGADLLISAGAIQSNHCRQTAAAAARFGFDCTLVLTGERPAQ
                     SSANFLLDQLFGAEIITVPDRKDRDRILQETFDAALAAGRKPYLVPYGGSSVTGAMAY
                     TFAVQEFMEQNRKVDWIVFGTSSGGTHAGFVLGQRLFGFKGRILGISIDEPEAELKAH
                     VSALASDASEKLGERINFSHDDVLATENYCQAGYGVFGEGEREAIKLFASAEGLLLDP
                     VYTGRAAAGMIDLIRRGFFKKDETILFWHTGGQPALFADKYSQDVVGKLPT"
     gene            complement(10541..10918)
                     /locus_tag="PLE39_15095"
     CDS             complement(10541..10918)
                     /locus_tag="PLE39_15095"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNE69707.1"
                     /translation="MTETKHNDEVLAIIKAATEVHRELGSGFLEAAYQEAMEIEADLM
                     GIPFVPQQNLQVQYKQHRLKKKYVADMICFDKIVVEFKVLDKLTDKEESQMAHYLKTS
                     GYKTGVLINFGSHPNLEWKQFAD"
     gene            complement(10951..12114)
                     /locus_tag="PLE39_15100"
     CDS             complement(10951..12114)
                     /locus_tag="PLE39_15100"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNE69708.1"
                     /translation="MNSSLWNQIISALPNPHFLQTYEWGQVKAKYGWVPFYAVWTADG
                     KWKAESDPQALSTLHSPVVAACLILKRTILNRGFAARLSVLYAPKGPLMDWGNASLRA
                     RVLDDLQSFAKKQGAVFLKIDPDVVLGRGIPHSEGDVLDNGGQMVMSELTRRGWGISS
                     EQIQFQNTVLIDLSASEDEMLMRMKPKTRYNVRLAEKKGVTVRAGKLDELGMLYRMYA
                     ETSVRDGFVIRDENYYLTVWKLFMQYRLPDFSFQPVALPLVAEVDRVPVAAIFLFMFA
                     GRAYYVYGMSRNLHREKMPTYLLQWEAMKRAKAAGCSVYDLWGAPEVFDESDSMWGVY
                     RFKEGLGGEVVRTLGAYDFAPNKLWYKLYADIMPRVLDVMRSRGKEKTKQGLE"
     gene            complement(12114..>12342)
                     /locus_tag="PLE39_15105"
     CDS             complement(12114..>12342)
                     /locus_tag="PLE39_15105"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNE69709.1"
                     /translation="WAKARGCEEYDLWGVPDEQEETLEANFETRRDGLWGVYRFKRGF
                     GGQLKRAAQALDRVYNPLLYWAYVKYMGNRE"
BASE COUNT         3069 a         3519 c         3024 g         2730 t
ORIGIN      
        1 gtcgcggtcg ggctcggcac cgacggcgaa aaagaaaata acaacctcga catgttcgaa
       61 gaactcaaga ccgcctccct gctggcaaaa ctctccgcgc tcgatgccgc cgcactggat
      121 gcatggtcca cctgccgcat gggaacggtc accggcgcac gtgccatcgg tctggattcg
      181 gagatcggct cgatcgagat cggcaaacaa gcggatttga tcgccgtccg caccgacaca
      241 ccgcgactca cgcctctcct ctcagggaca gctggcaacc tgcaccacaa cattgtccac
      301 gctgtcagag gcggcgacgt ggacatgacc atggtcgcag gcaacatcct cgtggacgga
      361 ggtcaactgc tcaccgccga cctgcgtcaa ctcattgacg aagccaacac cgctgtgcct
      421 ccgctcttca aacgccgcgc cgaatggctc gccacgcaca aatcggtcaa tgaactggtc
      481 aaacacaagg acgactagca cggtccgcac ttgaaagcgt atcccagcct tgctacaatg
      541 aatttggaaa agatcgcgag gtaaaaatga tctccatgat tcttggcacg cttacggtca
      601 ttgccctgtt gctggttgtg ggagccctga tccacctcaa atggacggaa accatttcag
      661 agagaaccat atcgcgcagg atcaaggcag agtaccccgc cgaggtccag gcgcaggttt
      721 accagaccta tcaacacatg aaggtgaagg aactcgaagg gctcttcctc aaaattctcg
      781 atgatgcaaa tggcgacgta ttgaaagttc aaaaactggc cagcatcgcc gaaagcgtga
      841 gctggcgcgc cttcctcgag aatcactggt aaagccgcat agtacctgcg tttgatatgc
      901 acgcccagcc gtatggctat aatccgatca aaatcttttg atggaggcat atcatggcaa
      961 ctttggaaca agaggagatc gccctcctta aagggcgcat cagcagactg gaagcccgcc
     1021 tggaatttct ctacaagcat ttgaatgtta cgtttgtgga agatacccgc ctgagcgacg
     1081 acccgaaagt gatcgcagcg ttaaaggcaa acaacatgct cgaagccatc aaattgtacc
     1141 gggccgcgac gaatacaagc cttgaagccg caaaagccgg tgtggaggaa atgcgggcaa
     1201 ggctgggtat ttaacaaagc ggtaaaacta ttttcaacag aaacagggat gcgggcatcg
     1261 catccctgtt tctgttgaat caacttacag attagtaaac cggtgaaatc gaagtccctt
     1321 tcacacccat ctatggttta atcgcccgaa acacaaaaag aattttacgc aaacagggag
     1381 agcaggatgc caaacatagc cgcttttgtt cgcaaggtct attttccatt ggtcagtttg
     1441 ctttgtttga tcggggtgat cggaacgggg gcactttcct ggcgcaccag ccaggatcaa
     1501 gatcagcgcc agcaggcatc ttttgaattt ggcagcgatc aagctgaacg ggtcgcaacc
     1561 gaggtcaacg cagaaatgaa cctgcaaatg accatcgccg aaacgatcgc aggtgaaatt
     1621 tccgatggca cactggccta cacggagatc accggccgcc tgcgcgatga actggatgcg
     1681 catcccaatt tttttggaat gggtgttgcc tttctcccgt atgtatataa atcagacctg
     1741 aaattatttg cgccctacta tttcaaaaac gagttcggga acttcacccg cgcccaggtg
     1801 gaaacagact acgattacag tgacagagcc acacaggata acgaatggtt ctatagcgcc
     1861 cttgaagacg gtgcctattg gtacgccagc gaatacgata tcaccgccca ggctgagttg
     1921 gtcgaataca ttaccccttt ttacagaacc aacccgcaaa caggtcagca ggaagccgtt
     1981 ggagtcgttt gggtggatca ctccgtcgag acgatgaaaa actttatccg ctcgatagac
     2041 atcggcgaag atgggtacac taccctgatc gaaagcgccg ggtacatcgt tgtccacccc
     2101 aaggcagaat acatcaacaa gaccctgtat gaggtggcgg aactggtggg cgacaccgag
     2161 ttaagcgccg cagcagaccg cgccatcagt ggcgaagatt tttacctgca gcgcaaaccc
     2221 accgtcaact tgaacagcac cgcatggaca ttcttcaaac cactgatagc cgaaggctgg
     2281 tcgatcgcgg tcaccatcag cgacaatgtg tttgaagccc cgccggaaga acttctgcgc
     2341 acacagatat ggttaagcat tatgatcatg accgccctca tttctctggc gttgctgatc
     2401 tttcgagtgg atgaaggtgc agtgcgtaat ctctggatcg tcgttggctg gagcaccttc
     2461 gttcttctca gcggcgtcat gtggatgtgg caaatcgtca acacgaatcc ctccaacatt
     2521 accgcatcaa aaaatatcct ggtcagcacc tcggttgtga atgaaagcct gaaatccgtg
     2581 gacgagaccg caacaataaa cggccgtccc attgccctgc gcatccccac cggggtcctc
     2641 gttcagaata tacagatcca gcccaacgaa gcggtgatct cggggtacct gtggcaaaaa
     2701 taccccaagg aactcccgcc caacttcatg gaacatctgg agcatgaatt cattcaaggc
     2761 gttgaatttc ccgacgacac aggggcaacg gcatttgaag aaagttatcg cttccaacgc
     2821 gccgattccg agatcgtcgg ctggttcttc accaccacac tgcatcaagc ctacaacacg
     2881 gcccaatatc cgctcgatca ggccaatatt cagatccgca tatcaccgca gtatctcgac
     2941 cagaacatcg tactggtccc agatttcgaa agctacgatt tcgtcgcccc ctcgtccctg
     3001 cccggtcttt ctccccagat caacattcca gattggaaca tccaacgtag tttcttcagc
     3061 tatcaacttc agcagttcaa cacaaatttt ggcagtcaaa cccacatcca aagaaacaac
     3121 ctgcccacac tcaccttcaa catcagtgcc agcagaaacc tgctctcccc catcattgca
     3181 ttctgcatca cgatcatcgt ggtggcgggg cttatgtttg gaagcctcat cgtcaaactc
     3241 gattcagcgt acagcgccct ctccaacgca gccgcgctct ttttcgtggt agccatcaca
     3301 cacgtggggc tgcgcggttc catcgctgct ggcggcacgg tttaccttga atactttttc
     3361 atcgtgcttt acattttcat cctcggcctc gccctcgatg gtttaatgta ttacaacgaa
     3421 agcgccattc ccatcatcag ctatggcgaa aacctcattc caaaactttg ctttgggccc
     3481 gggcttgcca tcacgttttt agcgatcacc atctttgtct tttatccgcg cacggtcgcc
     3541 gcagacccac tacaggaata tctctccatc aatgcgcttc aagcgactga agaacccgcc
     3601 gcatcggacc tgacgccaac cgcctccccc gtaacggaca gtggatcagc cgcgaataac
     3661 ctgacagcca ccccaatcag gccgtccacc tccccagccg ccaccaccgt tcccgtcatc
     3721 ctgcccgaag acccgatcac ccttcgttac ccctttggcc tcgaacctcc cacacttgac
     3781 ccttcccttg tcagcctcac ctcagaccag gatcaggttg agcagctctt catcggcctc
     3841 acccgtcttg agtctggcac caataccccg cttcccaacc tcgccgccag ttggacaacc
     3901 tccgaagacg gcctcacatg gaccttccac cttagaaacg acatcccctg ggtgcaatat
     3961 aacgccgcca caaacagcgt ctcacaggtg accgatttca acggcacgcc acgctttgtc
     4021 aatgcatatg acgtggtcta cggcattcac catgccctca cctccgaaaa tacatcggcg
     4081 gcgctgttat atgagatcaa aaacgccgaa caacccgatg acgacctcgg cgtaaaagcc
     4141 ctcgacgagt ggacattaga gatcaccctc aacagacccg cgccttactt tgccagcatc
     4201 gtcgcctatg acgtcagcta ccccgtccct ggctgggcca ttgaagaatg ggataaggaa
     4261 tggatcgccc ccgagaacat cgtcaccaac ggccccttcg tgctggcttc atgggaaaag
     4321 ggaaaaggca tcaccctgct caagaatccc ctctggccag aagcggataa aatccagatc
     4381 gaacgcgtgg aagagattct ggttcaggat ggagaaattg cgctaaaact gtatcgcgac
     4441 aatcaaatcg acaccctcgg cggcttgcgg agcaacatcc ttcaggaagt gaaaagtgac
     4501 ccgacacttg ccaatgaatt aagcagccat tccaacctgt gtgtacgtta tttcggtttt
     4561 acgaatacga aaccgccctt cgacgaccca cgtgtgcggc gtgcattctc cgctgctgtg
     4621 aaccgacagg agatcgtgga gatgctcaaa gtgggcaaca tcccagcttt cacctttgca
     4681 cccccaggaa ttttcggggc cgtaacagac ccaggcgttg ggcagggctt cgatgcagac
     4741 ctcgccaaac aactcttcca ggaatatctt gacgagcgcg gatttacgat tgccgatttc
     4801 aactctgcgt acccgcttgt gatgggacaa ctgaacgggc tcaacaactc cgcctccgat
     4861 atagccatca agaattggaa cgacatcctc ggcgtgaccg ttacgaccaa gacagtggaa
     4921 actgcagagt ttcagagcag cattgccaaa gacaacccta tcaaagatgc gttcagcatg
     4981 ttccaatggg catggtgcgc cgactacccc gaccaaaacg atttccttca cgtcctcttt
     5041 aattctgaaa ctggcacgaa cctcgtgcgc cgcaattgcg ccgatgaaac ctgcaccgcc
     5101 attctgccaa atgatgagtt cgacaacctg acattgatgg cctcccagat caatgacccc
     5161 gaagagcgcc agaggcttta cgcacaagcc gagcacattc tggttgtgga agaggctgca
     5221 ctggcaccga tctattacgg ggcatccacc agcctcacca agccgtggct gacgcgcaac
     5281 ttccccaaca tgggtgcgcc agatttcatg aactggatca tagatatttt ggcccaaaag
     5341 acaacaccat gagcatggca taaccttcca atggataaat ccctcactcc ctttttcaat
     5401 ccgcgcggcg ttgtcgtcgt tggtgcatcc acgtcacctg aaaaattggg gtatggggtc
     5461 gcgcggaatt tgatcgaaag caaatatcgg ggcgcgatcc atttcgtcag ccagaaaaaa
     5521 ggcgaattgt tcgggcaccc gctgcttctg agtctgaacg aggttcccga tcctgttgac
     5581 cttgcgatcc tcattgtccc gacccaatcc acaccgcaaa cgatcgaaga ctgtgccagg
     5641 cgcgggatca aggctgccat cattgtgtca tctggttttc gcgaagtcgg agcagaaggt
     5701 gccgcgctcg aagcccaatg tgtggagctt gcccacaaac acggcgtacg cctgctcggc
     5761 cccaactgca ttggcaccct cgatacacac ctgccattgg acacaacttt tttgcagccg
     5821 cccatgccca cgcagggtgg cattggcttt atctctcact cgggcgcgtt tgccgccgcg
     5881 gtcattgact gggcgcgtga acagggcttc ggcttttcgc agatcgtcag cctcggcaat
     5941 caggccgatg tcaacgagac cgatgttctc cctgcagttg ccaatgacga gcacaccaaa
     6001 gtcatcgcgt tgtacatgga aagcgtttca aacggggtga aattcgtgca agccgcaagc
     6061 gaagtcacca ggcacaaacc tgtcattgcg ttgaaggtcg gccgcttcga agcgggtcaa
     6121 aaagctgctg cctcacacac gggcgcactg gctggctccg aagcagcctt cgatgctgcc
     6181 tttgccaaag caggcgtcct ccgcgccgac accgccgagc agatgttcga ctgggcacag
     6241 gctttggaaa attgccccct gccgcgcggg aaacgcatgg cgatattaac caacgcaggc
     6301 ggaccaggcg tcatcgccgc cgattctctc gaaacgaacg gcctgctcct gtctcaatta
     6361 acagagtcca cgctgaaggc tttgacgagc tccctgcccc cctctgccag tgtccacaac
     6421 cctgtggata tgctcgcctc tgcctcaccc gatgcctacg ccacctgctt gaatcttctc
     6481 ctgcaagatg aaaccgtaga cggcgtgctc gtcatcctgc ccccgccgcc gatgtataaa
     6541 accgaagaag tggcgaaaaa aattattagg gtaattggca atttcgaaaa acctgttgtc
     6601 gttgccttga tgggttcgac cctggtggag aatgccagaa aagaattcca cgcggcaagt
     6661 gtcccgaact atccatttcc cgagcgcgcg gcatcggctc tgggagcgct ggcaaaacgg
     6721 gcagagtttt tcaccacaga gtcacagagg cacagagaat taaaaacccc gcgtctcggc
     6781 gcctccgtgg ttaatttatc tgctgatgaa ttagtctctg cctacggcat tccaaccacg
     6841 ccgatcaaac tggcgcggaa cgaaaacgaa gccgccgcca tcgcagacga acttggcttc
     6901 cccgtggtga tgaagatcgc ctcgcccgat attttgcaca aatccgatgt cggcggagtt
     6961 ttactcaacc tcacaacccc tcaaacgtta ttaagtgcct atacgcaaat gatggagcga
     7021 attaaatccg caatgccagc ggcgaaaatt gacggggttc atttgcagcg gcagatcccc
     7081 gaagggcagg aggtcatcat cggcatggtg cgcgatccgc tgtttgggcc gctcatgatg
     7141 ttcggttcag gcggcatcga agtggaaggt ctcaaagatg tggcgtttgc tttagcaccg
     7201 ctgaatcagg ccgaggcgcg gaaaatgatc cgcgagactt gggcaggtag aaagctgaaa
     7261 ggctttcgca gcatatcggc tgttgacgaa gagtctgtca tcgatgtcct catcaaactt
     7321 tcacagcttg cgatggagca ggaaaccgtg agcgagatcg agatcaaccc gttgcgggtg
     7381 ctgcccaaag gcgcggtggc tgtggatgtt cgagtaaaat tgcgcgcatg atccaactct
     7441 ccaatctcac gtattacccc atcaaagcct gccgcggatt tgacgttgcc gaatcgcagg
     7501 tggaacgcat ggggcttgcc aacgaccgcc gcatgatggt agtcaccccc tctggaaaat
     7561 ttctcaccca acgcgagtat ccgcgcctgg cactggtgac acccaccgta aaaaacggca
     7621 tggtcacact ttctgcaccg aattttgatt cgctgcagtt cgggattcaa agcacaggca
     7681 caccgacccc tgttgaggtt tggaaaagca aggatgtcag tgccatcgat cagggcgaag
     7741 aatctgcaca gtggctttca gattggctcg gtgtttcggt gcggctcgtt cacgtggctg
     7801 acggcttcaa aagaaaattg aatccagaat atgccgtcca cgccgatgac catactggat
     7861 ttgccgacgg ctacccgatc ctgatcatct ccgaagaatc gcttcaagac ttaaactcga
     7921 aacttgactc ggcggtcccg atgaatcgct tccgcccgaa cattgtggtc aaaggctgtg
     7981 aaccgtttgc agaagagacc tggaagcgca ttcgcatcgg cggcgtggaa atggcgcttg
     8041 tgaaaccctg tgcccgctgt gtcgtgacaa ccatagacaa agacaccctt gccaaaagca
     8101 aggaaccgct caagaccctg agttcctatc gaatgcagga attgggcgcc atcttcggcg
     8161 taaatgtcat tccgctgaac gaaggtgtgg tcaaggttgg catgaacgtg gaagttttgg
     8221 aatagcagca gaacgcgtta caccacgtca aatgagtaac catggaaaaa acgattattg
     8281 atattctcgg ctgggctggg acaattttat atctcgtcgc ctacgggttg atctctgcaa
     8341 aaaaagtgga gggtgattcg tggttgtatc agggcttgaa tatcgttgcg ggcacgtttc
     8401 tgatcattaa tactttctat ctgcgtgcct acccatcggc aggcttgaac atcgcctggg
     8461 tgggaatcgc aattgccaca ttgggccgca aagcttggac aaaggcttcg catgactagc
     8521 atttcacgca gagacttttt gaaactgggc ggagcggcat tgctcggcat ttcggcgggc
     8581 aaattcatcc cgccacagga agacgaaccc aatttcaccg cgccgcttat ttggagcggc
     8641 agccgcaaat ttaagcgcat ggcgttcaca tacgacgatt gctatctgct gaacaaaatg
     8701 caggaccttg aagagttgtt gaacgaattc cccgatttca aagtgacctt ctttcccgtc
     8761 gggtcgaaac tgctcaattt ggaagaacag gattcaggta tctggaagcg tctggtcgaa
     8821 aagggacacg agatcggtta tcacaccttt gaccatgtca acctcggcgt catgtcacct
     8881 gccactgcgt tgatggatta cgacaaatgg catgcagcct tgaatcaggt gctgggcatg
     8941 gaatacaccg tgcgattcgt gcgcccgcct tacgacatca tcagctacac catggatgta
     9001 ctctgccagg aacgcggatt ggtggcggcg atgttctcca tcggcggcgg cggcgaacct
     9061 gacatcgtga cccgtgcgat tcaaaaaggc aagggcggcg acattgtgca aatgcacatc
     9121 cgtacgcagg attacaaatc aagcgtacaa gtgtttccgt ggttgaaaga aaacagttgg
     9181 gagttggtga ccatgagcaa actctacgac gattacctgc gcgagcagat caaccccgaa
     9241 ggctgtgaat tagacgctgg aagttcattg acgcgggtgt gtgtggaata atatatagag
     9301 aatagtcaga agtaaaaaag aggcagccgc tttctacagc ggctgccttt accatttctc
     9361 tattttccaa ctagccaccc gacagtttgt ttttttgacc cgtgcaacaa gaaaaagact
     9421 aaccactgag gcacggagac acagagaaaa ccatcaaaaa actccgtgcc tcggtgtctc
     9481 cgtggttcaa atgtgttcat taactaaaac tgtcaggtag gtagttttcc aacaacgtct
     9541 tgagaatatt tatccgcaaa cagtgcgggc tggccgcctg tgtgccagaa caggatggtt
     9601 tcatcctttt tgaaaaatcc ccttcggatc aagtcgatca ttccagcggc ggcacggcct
     9661 gtgtagacgg ggtccaacag cagaccttca gcgctggcaa ataatttgat cgcctcacgc
     9721 tccccttctc caaaaacacc ataccccgcc tggcaataat tttcagtcgc caacacatcg
     9781 tcatggctga aattgatccg ctcaccgagt ttctcactgg catctgaagc cagcgcagag
     9841 acgtgtgcct taagttccgc ctctggctca tcaatgctga tccccagaat tcgccccttg
     9901 aagccaaaca gcctttggcc cagcacaaac cccgcatggg ttccgcctga agaagtgccg
     9961 aagacgatcc aatccacttt gcggttttgt tccatgaatt cctgcacggc gaaggtatag
    10021 gccatcgccc cagtgacact cgacccgcca tagggaacaa gatagggttt acggcccgct
    10081 gcaagggctg catcgaacgt ttcctgtaaa atgcggtcac ggtccttgcg gtctggcaca
    10141 gtgatgatct ccgcaccaaa aagttgatcc aaaagaaaat tagcagaaga ctgcgctggc
    10201 cgttcacctg tcagcaccaa ggtacagtca aaaccaaaac gggccgccgc tgcggcggtt
    10261 tggcggcaat ggtttgattg tattgcccca gccgaaatca gcagatcggc tccctgctcc
    10321 tgtgcctccg caacgaggaa ttccaatttg cgggttttgt ttccgccaaa agccagcccg
    10381 gtctggtcat cgcgcttgac caaaatacgc ggtccgccaa gtcgcgcggt taaatgagga
    10441 agttcttcga tcggcgtggg aaggtgggca aaattcaatc ggggaatggt tttcattggt
    10501 ctcctttatg atccataaaa acacaaaact gcatgaaagt ttaatctgca aattgtttcc
    10561 attcgagatt cgggtgactg ccgaagttga tcaacacacc cgttttatac ccagaagtct
    10621 tcaggtagtg ggccatctga gattcttctt tatcggtcaa tttatccaga actttgaatt
    10681 cgacaacgat tttgtcaaag cagatcatat ctgcaacgta tttctttttc aggcgatgct
    10741 gtttatattg aacctgcagg ttttgctgcg gcacaaaggg aatacccata aggtcagctt
    10801 cgatctccat cgcctcctga tacgctgcct ccaaaaaccc tgagcccaac tcgcgatgta
    10861 cttccgtagc tgccttgatg atcgcaagta cttcgtcgtt gtgttttgtc tcagtcattt
    10921 tgcctccaaa aaattaacgt ccagccgatt ctattccagc ccctgtttcg ttttctcttt
    10981 tcccctggaa cgcatcacat ccaacacgcg cggcatgata tcggcataga gtttgtacca
    11041 aagtttattt ggggcaaaat catatgcgcc aagcgtccgc accacttcac cgccaaggcc
    11101 ttctttgaag cgatacaccc cccacattga atcactttcg tcaaacacct cgggcgcacc
    11161 ccacaggtcg tacacagaac aacctgccgc ctttgcgcgt ttcatggctt cccattgtaa
    11221 aagataggtc ggcattttct cacggtgcaa attgcgcgac ataccataca cataatacgc
    11281 ccgccccgca aacataaaca ggaagatcgc cgcaacggga acacggtcaa cttcggcaac
    11341 gagggggagg gcaacaggtt gaaagctgaa gtctgggagt cggtactgca taaacagctt
    11401 ccagaccgtc aggtaataat tttcgtcacg gatcacaaag ccatcgcgca cactggtttc
    11461 ggcatacatg cggtagagca tccccaactc atccagcttg ccagcccgca ccgtaacgcc
    11521 ttttttctct gcgaggcgca cgttgtagcg ggtcttcggt ttcatgcgca tgagcatttc
    11581 atcttccgat gcggatagat cgatgagaac ggtattttgg aattggatct gctccgacga
    11641 aattccccat cccctgcgcg tcaattcgga catcaccatc tggccgccgt tatcgaggac
    11701 atccccttcg ctgtgaggga ttccgcgtcc aaggacaaca tcagggtcga tttttagaaa
    11761 gaccgcaccc tgcttcttcg caaaggattg caagtcatcc aacacacggg cacgcaggga
    11821 tgcattgccc caatccatca gcggaccctt cggcgcgtac aacacagata gccgtgcggc
    11881 gaatccgcgg ttgaggatgg tgcgtttgag aatgagacag gcggctacaa ctggtgagtg
    11941 taaagtggaa agcgcttgcg gatcgctctc cgctttccat tttccatccg ctgtccaaac
    12001 agcgtaaaac ggcacccatc catacttcgc ctttacctgc ccccattcat aggtttggag
    12061 aaaatgcggg ttgggaagtg cggaaatgat ttgattccaa agactactat tcatcactct
    12121 ctatttccca tgtacttcac atacgcccaa tacaacagcg gattgtacac tctgtccagg
    12181 gcttgtgcgg cgcgtttgag ttgaccgccg aatccgcgct tgaaacggta aacgccccaa
    12241 agcccgtcac ggcgggtttc gaaatttgcc tcgagggttt cttcttgctc gtcagggaca
    12301 ccccacaggt catattcttc acagccgcgc gcttttgccc ag
//
LOCUS       DAOQJC010000117        12158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2607_length_12158_cov_10.338511, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000117 DAOQJC010000000
VERSION     DAOQJC010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12158
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2607_length_12158_cov_10.338511"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PLE39_15110"
     CDS             complement(<1..586)
                     /locus_tag="PLE39_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69710.1"
                     /translation="MPINYQEIYTQVKQVGQGAKERRKKKEEAQELAQKLLETFSSEL
                     DALRSKVDSAKQADANIRCAVPLDEALASHYPTPDSVSATLIAADGSQAIADRHSAVQ
                     FCVINVGAISMKPDSGETPAVEVDSELFFGDAIEENGLTTDGGVALRRDLAERSVIEK
                     MSKGLKGSVVSFTDGTLEIFRAKDIDNANMYRNTV"
     gene            731..1453
                     /locus_tag="PLE39_15115"
     CDS             731..1453
                     /locus_tag="PLE39_15115"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNE69711.1"
                     /translation="MNKKILVFAILAFALILSACGPTTINQEAPAVVRTLNVNGLGQV
                     NLTPDIAYIYIGVHTEGATATEAVEANKTQTTALIASIKKSGVDEKDIRTTNFSIWPS
                     QQYGPDGTISGTVYMVDNTVNVTVRDLENLGDLLDSAISAGANTINSIQFDVADKVEA
                     LKEARAKAVADAQAQAQELAEAAGVSLGDIQTISFYENSVYPMDMGKGGGGAAYDAAS
                     AVPIQPGQLTVSVNVNMVYSIK"
     gene            1570..2508
                     /locus_tag="PLE39_15120"
     CDS             1570..2508
                     /locus_tag="PLE39_15120"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNE69712.1"
                     /translation="MKAGRVIIPYHSQSRSHFMLRVGYIGLGLMGKSIARNILKAGFP
                     VVVHNRSRGAVDELVAEGAVAANSPKEVAEQVDVVFTNLPDSPDVEKVALGENGILAG
                     GRVGLIVVDNSTIKPASARLVAEKLKEKGIFSLDAPVSGGDIGAKNGTLTIMVGGEVE
                     ALEKVMPVFQAMGKTITHVGDAGAGQVAKAANQIMVAAQMVAMGELLVFSKKAGVDPR
                     KVVDAIKAGAAQCWALDIKPPRLFDGNRNPGFKSYMQLKDMKIVLDTAKEYGVPVTST
                     EENTRFYQMMIDMGLGELDNSAVVGVIEKLAGVEII"
     gene            2556..3098
                     /locus_tag="PLE39_15125"
     CDS             2556..3098
                     /locus_tag="PLE39_15125"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNE69713.1"
                     /translation="MQINQPAPDFELPDLDGNLHRLSENRGKIVIVNFWSCECPHSER
                     TDKAIMAMFVQHLHRAAENDAVTEWRDEVKMLSIASNRNEHVEALKTAAEARRLPTVL
                     LDADCRVADLFEAQTTPHVFVVDREGILRYCGAVDDATFRNRMPTRFFLDLAVEALLE
                     GQLPALTESPAYGCAIVREI"
     gene            3111..4919
                     /locus_tag="PLE39_15130"
     CDS             3111..4919
                     /locus_tag="PLE39_15130"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE69714.1"
                     /translation="MIELKQRVMFLDKMHLFNGLKEEQLSGIGQKLTEREFSAGSVIF
                     KRGDKPDGFYMVYKGRVKVTRPRDVGEDFLAWLAPGDYFGEEALFENRNRSATITAME
                     NSTLLFLTREEFEKLLDRYDVLKPNFKVAIKSRKLARSTRFKWLGKNEVIYFIARRHK
                     LKLYQALVYPVLALAIPAGLFGLGVLTAATTPYALATVALVGILGWGVWNAIDWSNDY
                     YIVTNQRVIWLEKVIGLHDSREEAPLGTILSVGVETDAVGRVLDYGNVIVRTFVSRLE
                     FDYVDHPNQAADMIREYWERTKAITTRSQMDVMKDTIKQKLGLPLDKRPQVELSPVVP
                     EDQEKIAKTPYWILALSALFKLRVEEGATVIYRKHWIVYLQRGWRSLLTTLGVVFVFF
                     ARIWYFASSPTASFMKPNADGALRPDTLLLTIPVLMLPIGFWLWYEYTDWKNDIFKVT
                     SDEIIDLDRKPFGKEERRSAQIESILSTSYKREGLIANIFNYGTVNISVGGNQLAFED
                     VIDPAGVQADINRRRSARIAKKNEDAGKDDRERFATWLAAYHQNINEFTAPVKVVKED
                     TASTNQFTKFNPPGMEDMAAEDNEPDMGGTDMAGGE"
     gene            4991..5518
                     /gene="def"
                     /locus_tag="PLE39_15135"
     CDS             4991..5518
                     /gene="def"
                     /locus_tag="PLE39_15135"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNE69715.1"
                     /translation="MALREIVFLPEPVLRRKAKPVTKFDKELQTLIDDMIETMREAPG
                     VGLAAPQVNISQQLAVIEYDEDEDEEENEDAPPKPKKLYVIINPEIIKASEEKVMGIE
                     GCLSIPGLQGEVERHQSIQVKALNRHGSPVKLKVDGWMARIFQHEIDHLNGVLFTDRA
                     TRVWKPREDEDVPLD"
     gene            5532..5996
                     /locus_tag="PLE39_15140"
     CDS             5532..5996
                     /locus_tag="PLE39_15140"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69716.1"
                     /translation="MSIRLLTPDDLPRLREFWIERWGSDLMITRGESIRCDEVEGFVY
                     GEWLGLLTFLVRGDECEITSLDSLQAGQGIGSALLEAVLAEVKKRHCRRLFLITTNDN
                     LHALGFYQKRGFELAALYRGAVNESRKLKPSIPLLGENNIPLRDEIELEMQV"
     gene            5993..7261
                     /gene="recF"
                     /locus_tag="PLE39_15145"
     CDS             5993..7261
                     /gene="recF"
                     /locus_tag="PLE39_15145"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNE69717.1"
                     /translation="MNGKTSFQPFQTTAFQPVTYNPMYLKHLSLTNFRSLTRLDTELP
                     RRAIVLVGSNAQGKTSVLEAIYFLAAFTSFQTHVDRQIVNFHEAKNPLAVTRLVADYQ
                     RGKSKHHLEARLILEPTGVNGQRLRKEILLDGVKRHASDVIGHFNAVIFVPQMSQIVE
                     GGPDERRRYLNLALAQTVPAYARALSEYNQALTQRNALLKALSERGGNSDQLQVWDEA
                     LVKFGAQIILWRIQAVQEIERFASRIHHELTRGAEILRLAYEPAYDPLPKPNGQLGLK
                     LDTTIDRSSIELGEIQDGFLARLKQIRSEEIARGVTTIGPHRDDLRFIINKADVSDYG
                     SRGQLRTTLLALKLAEVEWMKERTGEWPVILLDEVMAELDTSRRADLLKYVSPDAQVL
                     FTTTDLTLFAPEFVQSAEVWNVKGGVVTSK"
     gene            7328..8872
                     /locus_tag="PLE39_15150"
     CDS             7328..8872
                     /locus_tag="PLE39_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69718.1"
                     /translation="MGRIRRDDFQSYKTDFFAMSHPWVMLSVAILLVNDYVLKVYFPS
                     WLTGKLSDLAGLFFFPILLSAVLNIVLEPLKFQPRHIALSSFWFTALWFTLIKTLPFF
                     SGLTGDILSSLMSLPVKIICDPTDMIALAMLFPAWKLRLSAGNGRMTKKRGLSYAMLF
                     IASLAAMASGPSGNIRVITRLVVHENKIYTYHDYSKDPYASNDPYASSSYEENYYSSD
                     GGQTWERSNETLPLQVVDQFMVPSKLPFTLCLPKAPNTCYRTGKETILESLDGGKTWN
                     VSWRIPLGREEFLSRILARHHLGPYDLVSLDYNGEQVVVAAIGLQGVLVKMSDAPWIS
                     VGVDGLGPIYFEARNINEAVDAIDEEIVALGLVAIVLFLVNAFFNVVRKGDQGVYFVA
                     VTLITFFGALGMLGLHFTPMGFTDASPPFLDGSSLLFLNGFAVVMFALQQILTGVGKQ
                     GARNLFVLFMTWLVSCSLFLLWAYGVIHVYETAKTIAVVLHVFSMLWVVYFFVRGPIQ
                     NKGPVL"
     gene            8980..9909
                     /gene="rbsK"
                     /locus_tag="PLE39_15155"
     CDS             8980..9909
                     /gene="rbsK"
                     /locus_tag="PLE39_15155"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HNE69719.1"
                     /translation="MSETGKYDVLVIGSLNADLVVRAPRFPQPGETISGDDLHTIPGG
                     KGANQAVAAARQGGRVAMLGRVGMDNFGDFLLENLKQNQVDVSNVQRDDASTGTAIIV
                     VDANGQNSIVLSAGANGHVSSADVDRASSLTPNLILLQLEIPTPTVLHAAQFAKTNGA
                     RVILNPAPAQTLPDDLIRLADFIIPNETELSLLTGLEVKDSASAEAAAKALLKRGVEN
                     VIVTLGSKGALVLSDTRVMQVDAYPIDVVDTTAAGDAFIGGFASSLLRGLDLKDAVRY
                     ANACGALAATKFGAQPSLPTKNEVETFMSLHDQ"
     gene            10036..11013
                     /locus_tag="PLE39_15160"
     CDS             10036..11013
                     /locus_tag="PLE39_15160"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HNE69720.1"
                     /translation="MNPKRIIIDTDPGVDDALTFLLALASPEIQLEALTTVHGNTNVE
                     NTTRNALAVLEFLHAENIPVAKGCSLPLLVPLHKSGDMVHGAGGIGNTNLPEPKNKPV
                     DEHAIDYLIQRVLAEPKELSIFPIGPLTNIALAIRKEPRFAEAVKELVIMGGSLRAGG
                     NITPAAEFNLHADPHAAHIVFHSGIPITLIPLDVTYKCLLTSEDVERLNRTGSPIARF
                     VRDATGVYMEFYKKYEGFDGCALHDPLTLATIIAPELLTFENHYVDVDISGGISMGNT
                     LADFMKVSKKPANVKVALGVRGREFVELFIERMETLSASVGTHTAEDGG"
     gene            11040..11987
                     /locus_tag="PLE39_15165"
     CDS             11040..11987
                     /locus_tag="PLE39_15165"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HNE69721.1"
                     /translation="MPKHIILDTDPGIDDSLAILLALASPELVLDGISTIHGNASTAQ
                     VTRNALAILELAKAGHIPVYAGCELPLVQPSLLSPETHGDSGLGYAKLSEPLGRPQLK
                     HGSDFLIERIMSSVEDVTLVAIGPLTNVAMAIRKEPRIVEKVKEVFIMGGAIRHQGNT
                     TPLAEYNTFVDPHAAHIVFHSGMPMILTPLDVTYQCIFLKEDLDRLLKIDSPITKFIA
                     DATRFYMEFHDEYQKIDGCVINDPMTLALTFMPEICDYESLYVDVDISTGVGLGNTFA
                     DFYNYEKKKPNMKVALGVRPRMFMELFLERMEKLARLLE"
BASE COUNT         2899 a         3012 c         3280 g         2967 t
ORIGIN      
        1 ccacggtgtt gcggtacata ttggcattgt caatgtcttt agcacggaag atttcaagcg
       61 tgccgtctgt aaatgaaacg accgagcctt tcaagccttt gctcatcttt tcaataacgc
      121 tgcgctcggc aaggtcgcgc cgcagggcaa cgccgccgtc ggtggtgagt ccgttctctt
      181 cgatggcgtc gccgaagaag agctcgctat ccacttctac agctggggtt tcacctgaat
      241 caggtttcat agagattgcc cccacattga tgacacagaa ctgaaccgcg ctgtgtctgt
      301 ctgcaatggc ttgcgaacca tcagcggcga tgagagttgc agaaacagaa tcaggcgtcg
      361 ggtagtgaga agcgagggct tcatccaacg gcaccgcgca gcgaatattg gcatccgcct
      421 gtttggcaga atccactttc gagcgcagtg catccaattc agaagagaag gtttcgagga
      481 gtttctgcgc gagttcctgc gcctcctctt tcttctttct tctttccttc gcgccctgcc
      541 caacctgctt gacctgtgtg tagatttcct gatagttaat tggcatagaa catttgtgca
      601 aaattttagc cgtaaaccaa ccaaaataca aggtggtgcg gttctcttaa aaataaataa
      661 gtcatcccca gacgggaact ttttttgaag atgggtcgtc tgataggcat aacacttaag
      721 gagacataca atgaacaaga aaattttggt atttgcaata ttggccttcg ccctcattct
      781 gagcgcctgc ggcccaacca cgatcaatca ggaagcacct gccgttgttc gcacattaaa
      841 cgtcaacgga ctcggacagg tcaacctcac acctgatatc gcctatattt atattggtgt
      901 tcacacagaa ggtgcaaccg ccactgaagc agtggaagcg aacaagaccc aaaccactgc
      961 gctgatcgct tcgatcaaga aatcaggcgt ggatgaaaaa gatatccgca ccaccaattt
     1021 cagcatctgg ccttcacagc aatacggccc cgatggcacg atcagcggca ctgtttacat
     1081 ggtggataac acggtcaatg tgactgtgcg tgatcttgaa aacctcggtg acctgctcga
     1141 ttcagccatc agcgcgggtg ccaataccat caacagcatc cagttcgatg tagcggacaa
     1201 ggttgaggct ttgaaggaag cccgtgccaa agccgttgca gatgcgcagg ctcaggcgca
     1261 ggaactggcc gaagccgctg gcgtttcact cggcgacatt cagaccatca gcttctatga
     1321 aaatagcgtt taccccatgg atatgggcaa aggtggtggc ggtgcagcct atgatgcagc
     1381 ctcggctgtg ccgatccaac ctggtcagtt gacggtgtct gtgaacgtca acatggtgta
     1441 ctcgatcaaa taaacgcatc acccttaacc gaaacctgct cctgaacaag gagcaggttt
     1501 tacgtttcat cctcctctcc tgaatgccgc aaactgcggc tgcgtttggg agaggtgcct
     1561 ttttgggcgg tgaaggctgg gcgcgttata atcccgtatc attcccaatc aaggagtcac
     1621 tttatgttaa gagttggata catcggtctt ggattaatgg gcaaatccat cgcgcggaat
     1681 attctcaagg cgggttttcc tgtggtcgtg cataaccgtt cccgcggcgc agtggatgaa
     1741 ctggtcgctg aaggggccgt tgcggcaaat tcgccgaagg aagtggccga acaagtggat
     1801 gtggttttca caaacctgcc tgattcaccc gacgtggaga aagtggcatt gggagaaaac
     1861 ggaatcctcg cgggtgggcg cgttggcctc attgtggtgg ataactccac catcaaacct
     1921 gcttctgcgc gtttggtcgc agagaagttg aaagagaagg gaatcttctc gttggatgcc
     1981 cctgtttcag gcggcgatat tggcgcgaag aacggcacac tgaccatcat ggtcggtggc
     2041 gaggttgaag cgctggagaa ggtgatgcct gtattccaag cgatgggcaa aaccatcaca
     2101 catgtgggtg atgcaggtgc cgggcaggtg gcgaaggctg ccaaccagat catggtcgcc
     2161 gcacagatgg tggcgatggg tgaattgctg gtcttttcca aaaaagctgg cgttgacccc
     2221 cgcaaagtgg tggatgcgat caaagctggt gcagcgcaat gctgggcgct tgatatcaaa
     2281 cccccgcgtt tgttcgatgg caaccgcaac cctggcttca aatcgtacat gcagttgaag
     2341 gatatgaaga tcgttttgga taccgcaaaa gaatacggtg tacccgtcac ctccaccgag
     2401 gaaaatacaa ggttctacca aatgatgatc gatatggggc tgggcgagct ggataactct
     2461 gccgtggtgg gtgtgatcga aaaactggcg ggtgttgaga tcatctaaac gttaacacgt
     2521 ttgaacgttc caacgtttaa aacctgtaaa ctcgaatgca aatcaaccag cctgcccctg
     2581 atttcgaact gcctgatctt gatggcaatc ttcaccgctt gagcgagaat cgcgggaaga
     2641 ttgtcatcgt taatttctgg tcgtgtgagt gtccgcattc ggagcgcacc gacaaggcga
     2701 tcatggcgat gttcgtgcag cacttgcacc gcgctgccga aaacgatgca gtgacagagt
     2761 ggcgtgacga ggtaaaaatg ctgtccatcg cgtcgaatcg aaatgagcat gtggaggcgt
     2821 tgaaaactgc cgctgaggcc cgccgcctgc ccacggtcct gttggatgcg gattgccgcg
     2881 tggcagatct gtttgaggcg cagaccactc cccatgtgtt tgtcgtggat cgggagggaa
     2941 tccttcgcta ctgtggcgca gtggatgatg ccacctttcg gaatcggatg cccacccgtt
     3001 tctttctgga cctggcggtt gaggcgctgc tcgaagggca gttgccagca ctgacagaat
     3061 ctcccgccta tggctgcgcc attgtccgtg aaatttgata gaatcaaaac gtgattgagc
     3121 tcaaacagcg cgtgatgttt ttggataaga tgcacctctt caatgggttg aaagaggagc
     3181 agttatctgg cattggtcaa aaactgaccg agcgcgaatt ttctgcgggg agtgttattt
     3241 ttaagcgcgg cgataaaccc gatggctttt acatggtcta taaaggcagg gtcaaagtga
     3301 cccgtccgcg tgatgtgggg gaggattttc tggcgtggct ggcaccgggc gattattttg
     3361 gcgaagaagc cctgtttgaa aaccgaaatc gttcggccac catcaccgcc atggaaaata
     3421 gcaccctgct tttcctcacg cgtgaggaat ttgaaaaact gcttgaccgt tatgatgttc
     3481 tgaagccgaa tttcaaagtg gccatcaaga gccgcaagct ggcacgttcg acccgtttca
     3541 aatggctggg taagaacgaa gtcatttatt ttattgcccg cagacataaa ttgaagttgt
     3601 atcaggccct ggtgtatccg gtgcttgcgc tggcgatccc tgccgggttg tttggattgg
     3661 gtgtgttgac agccgccacg accccctatg cactggctac tgtggctttg gttggcatcc
     3721 ttggttgggg ggtgtggaat gccattgact ggagcaatga ctattacatc gtcaccaatc
     3781 aacgcgtgat ctggttggag aaagtgatcg gcctgcatga cagccgcgaa gaagcgccgt
     3841 tgggcacgat cctttctgtg ggtgtggaaa ccgatgcggt tggccgtgtg ctggattatg
     3901 gcaatgtgat cgtgcgtacc tttgtcagca ggcttgaatt cgattatgtc gaccacccca
     3961 atcaggccgc cgatatgatc cgcgaatatt gggaacgcac aaaagccatc accacccgca
     4021 gccagatgga tgtgatgaag gacaccatca aacaaaaatt gggcctgccc ttggataaac
     4081 gtccgcaggt ggagctttcc ccggtggtgc ccgaagatca ggaaaagatc gcaaaaacac
     4141 cttattggat tcttgcgctc tccgcccttt ttaaattacg tgtcgaggaa ggcgccaccg
     4201 tcatttaccg caaacattgg atcgtgtact tgcaaagagg ctggcgttca ctgttgacca
     4261 cgctcggtgt cgtttttgtg ttctttgccc gcatctggta ttttgcgtcc tcgccgaccg
     4321 catcctttat gaagccgaat gccgatggtg cgctcaggcc cgatacgctt ttgttgacca
     4381 ttccggtgtt gatgctgccg atcggttttt ggctatggta tgaatacacc gactggaaga
     4441 acgatatctt caaagtcaca tccgatgaga tcatagacct cgaccgcaag ccttttggta
     4501 aagaggaacg ccgctctgcc cagatcgaaa gtattttgag cacctcttat aaacgcgaag
     4561 gattgatcgc caacatcttt aactatggca cggtcaatat ttcggtgggc ggcaaccaac
     4621 tggcctttga agatgtgatc gatcctgccg gggttcaggc ggatatcaac cgcaggcgtt
     4681 cggcacgtat tgccaaaaag aatgaagatg caggcaagga tgaccgcgaa cgctttgcca
     4741 cctggctggc ggcctatcat cagaacatca acgaattcac cgctccggtg aaggtggtaa
     4801 aggaagatac cgcctccaca aaccaattta caaaattcaa tccgcccggc atggaagaca
     4861 tggccgccga agataacgaa ccggatatgg gcgggactga catggcgggt ggggaatagc
     4921 ctgcaatgtg cggtatcatt gcggctcaaa atgaccaaag acaagtttta gcaaaaagga
     4981 agaccaaaga atggctttaa gagaaatcgt attcctgcct gagcctgtat tgcggcgcaa
     5041 ggccaaaccc gtgacgaaat tcgacaagga attgcaaacc ctcatcgatg acatgatcga
     5101 aaccatgcgc gaggcaccgg gcgtcggcct ggccgctccg caggtgaaca tttcgcagca
     5161 gctggctgtg atcgaatatg acgaggatga agacgaagag gaaaatgaag atgcccctcc
     5221 gaagccgaag aagttgtatg tcatcatcaa cccggagatc atcaaggctt ctgaagaaaa
     5281 ggtgatgggg attgaaggct gcctttcgat tcccggcttg caaggcgaag tggaacgaca
     5341 tcagtccatt caagtgaagg cgctcaaccg ccatggcagc cctgtcaaac tcaaggtgga
     5401 tggctggatg gcgcgcattt tccaacatga gatcgaccat ttgaacggag tcctcttcac
     5461 agaccgtgcc acccgcgtct ggaagccaag agaggatgag gacgttccgc tcgattagca
     5521 attccctgaa aatgagcatc cgcctcctca ctcccgatga cctgccccgc ctacgagaat
     5581 tttggatcga acgctggggc agtgacctga tgatcacacg cggcgaatct atccgttgtg
     5641 atgaagtgga ggggtttgtc tacggtgagt ggctcggcct gttgaccttt cttgtccgcg
     5701 gtgatgaatg tgagattact tcgctcgata gtttgcaggc tgggcaggga attggctcag
     5761 ccctgctgga agcagttctg gcagaagtaa aaaagagaca ttgccgccgt ctgtttctca
     5821 tcaccaccaa cgacaacctg cacgcgctcg gcttttatca aaaacgtgga ttcgaactgg
     5881 cggccctgta tcgcggtgcg gtcaacgaat cgaggaagtt gaaaccgtcc attccattgc
     5941 ttggagaaaa taatattccc ctccgcgatg agatcgaact tgagatgcaa gtttgaatgg
     6001 caagacaagt tttcagcctt tccaaaccac cgccttccaa cccgtaactt acaacccgat
     6061 gtacctcaaa cacctctccc ttaccaactt tcgcagtctc acccgcctgg atactgaact
     6121 tccacggcgg gcaattgtgt tggtggggag caatgcgcaa ggcaaaacca gcgtgcttga
     6181 ggcgatctat tttctggcgg cattcacttc cttccaaacc catgtggacc ggcagatcgt
     6241 caattttcac gaagcaaaga atcccctcgc tgtgacccgc cttgtggcag attaccagcg
     6301 tggaaaatcg aaacatcatc tcgaagcgcg gctgatcctt gaacccactg gcgtgaatgg
     6361 gcaaagactc cgcaaagaga ttctgctgga tggggttaag cgtcacgcca gcgatgtcat
     6421 cgggcatttc aacgcggtca tttttgtgcc gcagatgtcg cagatcgtgg agggcggccc
     6481 tgatgaacgc aggcgatatc tcaaccttgc cctggcacaa actgtccctg cgtatgcgcg
     6541 ggcgctcagc gaatacaatc aggcgttgac ccagcgtaac gccctgctca aggcgctgag
     6601 tgaacgagga ggaaacagtg accagcttca ggtgtgggat gaagcgttgg tcaaattcgg
     6661 ggcgcagatc attttgtggc ggattcaagc cgtgcaggag atcgagcgtt tcgcgtcgcg
     6721 catccatcac gaattgactc gcggtgccga gattttgcgc cttgcctatg aacccgccta
     6781 cgaccccctg cccaaaccga atggacaact gggcttgaaa ctggatacaa ccattgaccg
     6841 ttcatccatt gagttgggtg aaattcagga tggattcttg gcgcggctca aacagatccg
     6901 cagtgaagag atcgcgcgtg gcgtgaccac gatcggcccg catcgcgatg acctgcgatt
     6961 catcatcaac aaagcagatg tgagcgatta cggttcgcgt ggacaactcc gcacgaccct
     7021 gctcgcgctt aaactcgccg aagtggaatg gatgaaggaa cgcaccggtg aatggcctgt
     7081 gattctgctc gatgaagtga tggccgaact tgataccagt cgccgtgctg acctgctgaa
     7141 atacgtcagc cccgatgcac aggtgttgtt taccacaacg gatttgactt tgttcgcgcc
     7201 ggagtttgtg caaagcgcgg aggtttggaa tgtgaagggt ggtgtggtta cgtcgaagta
     7261 gcaatgaggt tattttcata attaactgaa aatgggtgaa atcaagaaaa tgggataggt
     7321 gatgacaatg ggcaggatta gacgcgatga ttttcaaagt tataaaaccg atttttttgc
     7381 catgagccat ccatgggtga tgctgagtgt tgccattttg ttggtcaacg actatgtctt
     7441 gaaggtatat tttccttcct ggcttaccgg caaactcagc gaccttgcgg ggctgttttt
     7501 ctttcccatt ctcttgagtg cagtattgaa tattgttctt gagccgttga aatttcaacc
     7561 acgtcacatt gctctttcgt ccttttggtt taccgcatta tggtttactt taatcaaaac
     7621 cctgccgttc ttcagcggcc tgactgggga tattctttcc tctctgatgt cgctccctgt
     7681 aaaaattata tgtgacccga ctgacatgat tgcattagca atgctttttc ctgcatggaa
     7741 attgcgattg agcgcaggga atggcaggat gacaaaaaag cgcgggctgt catatgcaat
     7801 gttgtttata gcatctcttg cagccatggc ttctggccct agtggcaaca ttagggtaat
     7861 aacgcgcctt gttgttcatg aaaataaaat ctatacgtat catgactact ctaaagaccc
     7921 ctacgcctct aatgacccat acgccagcag tagttatgaa gagaattatt acagctctga
     7981 tggcgggcag acatgggaaa gatcgaacga aacactccct ttgcaagtcg ttgatcaatt
     8041 tatggttccc tcaaaattgc ctttcacgtt gtgcctgccg aaagcaccga acacgtgtta
     8101 ccgcacaggc aaagaaacga ttctcgaatc ccttgatggc ggaaaaacat ggaatgttag
     8161 ttggcgcatt ccattgggca gggaggagtt tttgtctcgc attctggcaa ggcatcatct
     8221 tgggccgtat gatctggtct ctcttgatta caatggagaa caggtggttg ttgcggccat
     8281 tggtttgcaa ggcgtcctag tcaaaatgag cgatgctcct tggatttctg ttggggtgga
     8341 cgggttgggt ccaatttatt ttgaggcaag gaatataaac gaggcagtgg atgccataga
     8401 tgaagagatc gtcgccctcg gcctggtggc tatcgtgctt tttctggtca atgcgttttt
     8461 taatgtcgtg agaaagggcg atcagggtgt ttacttcgta gcagtaacat tgattacgtt
     8521 ttttggtgcg ttaggtatgc taggacttca cttcaccccc atgggcttta ccgacgcgag
     8581 cccgcctttc ctagacggtt cttccttgct ttttttgaat gggtttgccg tggtcatgtt
     8641 tgccctccag cagatactca ctggagtggg aaaacaaggc gcaagaaatc ttttcgtgct
     8701 tttcatgacc tggctggttt cctgctcgct ttttctcctt tgggcatacg gcgttattca
     8761 cgtgtatgaa actgcgaaaa caatagccgt tgttttacat gtgttttcta tgttgtgggt
     8821 tgtctatttt tttgtcaggg gaccaattca aaataaagga ccagttttgt agcttcgcgt
     8881 gaaatacact gtgagtacat agatataatg ctcccaatga ttcaattttt cgatgccgct
     8941 ttcgacatca cgcgataacg atagtgtaaa attgcccgta tgtctgaaac agggaaatac
     9001 gatgttcttg tcatcggttc gttgaacgcc gacctcgtcg tccgtgcgcc gcgatttccg
     9061 cagcccggcg aaaccatcag cggtgatgac ttgcacacca ttcctggcgg caagggggcc
     9121 aatcaggctg ttgctgcagc gcggcagggt ggcagggtgg ccatgttggg gcgcgtgggc
     9181 atggataact tcggcgattt cctgctcgaa aacctcaagc aaaaccaagt ggatgtttcc
     9241 aatgtccagc gtgatgatgc ctccacggga acggccatca tcgtggtgga tgcaaacggg
     9301 cagaacagca tcgtgctatc tgctggagcg aacggtcatg tctcttctgc ggatgtggac
     9361 cgcgcctctt ccttaacccc aaacctgatt ctgcttcaat tggaaattcc caccccgacg
     9421 gtgcttcacg ccgcgcaatt tgccaaaacg aatggtgcgc gtgtcattct caacccagcg
     9481 cccgcgcaga ctctccccga tgacttgatc cgccttgccg actttatcat tcccaatgaa
     9541 accgaattaa gcctgttgac cgggcttgag gtcaaagaca gcgcctctgc cgaagcggct
     9601 gcgaaagccc tgctcaaacg cggcgtagag aatgtcattg ttacgttggg aagcaaaggg
     9661 gcattggtct tatccgatac acgggtgatg caggtggatg cctatccaat tgacgttgtc
     9721 gataccacag ccgcaggcga tgccttcatc ggcggattcg cctcatctct cctgcgcgga
     9781 cttgacctca aagatgcggt tcgatatgca aatgcctgtg gcgcgctcgc tgcaaccaaa
     9841 ttcggcgcac aaccatccct gccaacaaaa aacgaagtgg aaacctttat gtcattgcat
     9901 gaccagtgac ctttcgcccg aagcgatctc atcgcggccg ttgagaccgc tcaccacatg
     9961 aactgcctgt caattcatgt gcggcgcaag tgttcccaga tcatcgctct cctcgcaatg
    10021 acggggaaaa aataaatgaa cccaaaacga atcatcatag acaccgaccc cggcgtagac
    10081 gacgcgctga catttctcct tgcccttgcg tcccctgaaa tccagctcga ggcattgacc
    10141 accgtccacg gtaacacgaa tgtggaaaat accacgcgca atgcgctggc tgtattggaa
    10201 ttccttcacg cggaaaatat ccctgtggcg aaggggtgtt cgttgccgtt gctcgttcca
    10261 ctgcacaaat caggtgacat ggtgcatggt gcaggtggaa tcggcaatac aaacctgcca
    10321 gaacccaaaa acaaacccgt tgacgaacat gcgattgatt atttgatcca gcgtgtgctg
    10381 gccgagccga aggaactcag catttttccc atcggcccgt tgaccaatat tgcgctggcc
    10441 attcgcaaag aaccgcgttt tgccgaagcc gtgaaggaac tggtcatcat gggtggctct
    10501 ctgcgtgcgg gcggaaatat cacccccgct gctgaattca acctgcatgc cgacccgcat
    10561 gccgcgcata tcgtttttca ctcgggaatt cccattacgt tgatcccttt ggatgtgacc
    10621 tataaatgcc tgctcacttc tgaagatgtg gagcgtttga acagaacggg ctcgcccatt
    10681 gcccgatttg tccgcgatgc gacgggggtt tacatggagt tttacaagaa atacgagggc
    10741 ttcgatggtt gtgcgctgca cgatccgctg acattggcga caatcattgc gcccgaactg
    10801 ctaaccttcg agaatcatta cgtggatgtg gatatttctg gcggaatttc gatggggaac
    10861 acactggccg atttcatgaa ggtttcgaag aaacccgcaa atgtgaaggt tgccctcggt
    10921 gtgcgtgggc gcgaatttgt ggaattattt attgagcgga tggaaactct gtctgcttcc
    10981 gtcggcaccc acaccgccga ggacggcggc tgagcatagg aaggtacaga gaaaaaatca
    11041 tgcccaaaca catcatttta gacactgacc ccggtattga cgactctttg gcgatcttgc
    11101 tcgctctggc ctcgcccgaa cttgtcctcg atggaatcag cacgatccac gggaatgcat
    11161 ccacggcgca ggtgacgagg aatgcgctgg cgattttgga actggcaaag gcggggcaca
    11221 tccctgttta tgcgggctgt gaattgccgc ttgtgcagcc gtccctgctc agcccggaga
    11281 ctcacggcga cagcggcctg ggttatgcaa aactgtcaga accgctgggc aggccccagc
    11341 ttaagcacgg aagcgatttc ctcatcgaga ggatcatgtc gtcggtggag gatgtcacgt
    11401 tggtggcgat cggtccgctg acgaatgtcg ccatggcgat tcgcaaggag ccgcgcattg
    11461 tggagaaggt gaaggaggtt ttcatcatgg gcggggcgat tcggcatcag ggcaatacca
    11521 cgccgctggc ggagtacaac acctttgttg acccgcatgc cgcgcacatc gtttttcatt
    11581 cgggcatgcc gatgatcctc acgccgctgg atgtaaccta tcaatgtatt tttctaaaag
    11641 aggatctgga caggttgtta aagatcgatt cgccgatcac gaagttcatc gcggatgcga
    11701 cccgctttta catggagttc cacgatgaat accaaaagat agatggctgt gtgatcaatg
    11761 acccgatgac tctggcgttg acgttcatgc ctgaaatctg cgattacgaa tctctttatg
    11821 tggatgtgga tatctcgaca ggcgttgggc tgggaaatac gttcgctgat ttttataact
    11881 acgaaaagaa gaagccgaat atgaaggtgg ccctgggggt tcgtccgcgc atgttcatgg
    11941 agttgttttt ggagcggatg gaaaagctgg cgaggttgtt ggaatgacga tctttcacca
    12001 cagagcccac aggggccaca gagaaaaaaa agactccgtg ctctttgtgc tctctgtggt
    12061 taagtcttta aactgccagc ctatattttc gtgaccattg agattgaaaa gtgttatgat
    12121 gttcatacag atccattcac attttttcgg aggagatt
//
LOCUS       DAOQJC010000118        11993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2661_length_11993_cov_9.321494, whole genome shotgun sequence.
ACCESSION   DAOQJC010000118 DAOQJC010000000
VERSION     DAOQJC010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11993
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2661_length_11993_cov_9.321494"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="PLE39_15170"
     CDS             complement(<1..945)
                     /locus_tag="PLE39_15170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69722.1"
                     /translation="MTEKIYLDPSLTLRRARWTDVNAVAKLNHDVAEMEGDSLFVLTA
                     EELANEWENEGFNVERDVFVVETQDGRLVGSEEFYNESGHYKLKADGCVHPEFRGLGV
                     GSSLLEKIAERARAEIELAVPNVQVTIQSLINNKDEAGHNLLKNNGYSLVRYFWRMEI
                     KLQEAQPALNLPAGIELRPFIKDEHAVAVWQADNEAFRDHWGSHDRTFQEWSHAKFGN
                     PNFDPTLWMIAWDGNEVAGFSQNRFRKGIGWIGTIAVRRPWRGKSLGIALMRQTFGEF
                     YKRGTTIIGLGVDSANLTGATRLYERAGMYVAGEFALYE"
     gene            complement(1037..1681)
                     /locus_tag="PLE39_15175"
     CDS             complement(1037..1681)
                     /locus_tag="PLE39_15175"
                     /inference="COORDINATES: protein motif:HMM:NF039197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5694 domain-containing protein"
                     /protein_id="HNE69723.1"
                     /translation="MTSENQEYLERFTDQISKFMPTAVLLEFDPVDEKAVQEKYDQYL
                     TGSFTLPSNEIYQLGFRIAKKSSLKTVYSFDERTIGWNAGPMFEYINTHDAETKSRFD
                     ALIEQIGETTKRAHETLSLAELLMDANAEETDRHNKYIYLITNHVGAGDNFEGADAAA
                     SWWHRNFRMYANIQKHAAPGERVLAIGGQGHTAILKDLLKIDEDREAVDFAQYL"
     gene            complement(1865..3970)
                     /locus_tag="PLE39_15180"
     CDS             complement(1865..3970)
                     /locus_tag="PLE39_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69724.1"
                     /translation="MAEPLYFQWQNTVLCKTIYPMREPKLRDFLVYYMEIDLWKQYKD
                     KDISTLKTEVDAYITAQEFALVAAYKSYSALRSYFMLKDVRGYYLKYKPINEAELTEI
                     NNFHAIFIEGWPKDIRGERSFVEGQLQYWVNHRNMQKEAAKSKRRRVENMDPAHPKRP
                     IEAQELETMEGITLPMAEQELDQVRAFLKTYDKVEQRKLAWYKQSKADPAFSTSESEF
                     LVHYKPEAPITVQDIARWKVEEYKTSLEKKNQYELLEEIYQRFKKEPNRYPHWLQYMV
                     VHFSGMRYASAHNSWADPKLLLMRLRGSNAGSEIRKLDDAAVEKLCTEKIAVYETGTG
                     SNIPKLARASEKEWKDKVALHMLSVKASGPKTRRNGLTALQAEELNYEINSLSTEEAL
                     KALEDMKSTFPAWAWKEIVKLTPLRVNHVTDPNWEKLTPEEEAARYSQTYNDIRNVLD
                     AWENYDSSAWREEHGRSHELIVSRAVCNETAEHCQHLRGHLPPGGLTPKPKWYFNNET
                     EGKLPGDPRPFYTRPTSAADYVPGASIFWLRFVDKEPNAWQVAKEVKTKSGVGLLPDM
                     GKKKGDKSDPKATSTPWEYQKGETVTRSRTLINPETKVKSQQHQWLRWIHEATVAEVA
                     ETADGTMVITYETALPDDDKGTSSVGTFRMPLSWHLDDGPEDTYNRSFVGFVPEGQVP
                     MEHLKTMMDWNRILRKKVV"
     gene            4135..4590
                     /locus_tag="PLE39_15185"
     CDS             4135..4590
                     /locus_tag="PLE39_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69725.1"
                     /translation="MEDRELYGKSAIFGMGLGALLGLILGGATGALSLTWGGIQLAGL
                     VGLAAGAVTGALTGLISARVAGRTGGVSFGAYGGMAFGGFLGLILGAFIPASFRESIA
                     TDQMPYLTVLANGTFEMAILVSFLLACLGTAVGAWVAGRNFIPRDQREG"
     gene            4596..4952
                     /locus_tag="PLE39_15190"
     CDS             4596..4952
                     /locus_tag="PLE39_15190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNE69726.1"
                     /translation="MDKTFIKDLLVRGIIGIRDWERVTPQDILINVTVFSDTRLAAQT
                     DDIAHCVDYSALAKKVQAHAETSARLTVEALANDLAEICLKENGAQKVIVRVEKPSAV
                     RFAKSVGVEIERSRNE"
     gene            complement(5073..7889)
                     /gene="leuS"
                     /locus_tag="PLE39_15195"
     CDS             complement(5073..7889)
                     /gene="leuS"
                     /locus_tag="PLE39_15195"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNE69727.1"
                     /translation="MAEKKKTTVKKKTTAKAVASKTKKVVKKAATKAKPAAKKTVVKS
                     KPVAKKAVTKKSVAAKKAPVKKAAPKAAKKTTKAIVPAQKMAVVVAKEKPKAVVPVTE
                     KPKAIVPVKAADSAPVQQDKSYNPKNIEKKWQDQWDADQLYRSVIDPSKPKHYALTML
                     PYPSGDLHIGHWYAMTPSDARARYMRMKGYNVLFPMGFDAFGLPAENAAIKDGVHPMK
                     RTFQNIERMRKQMRSMGAMFDWKREAVSADPAYYKWTEWFFIQLYKAGLAYRKMSAVD
                     WCPKCNTTLAREQVWGDDRHCERCGTPVIKKDLDQWFFKATKYADELLSFEGIDWPER
                     VKTLQTNWIDRSEGASVIFKTEQGDDVEIFTTRPDTLWGATFMVFSPEHPLVEKITTE
                     ENKATVNEYKLQAARQTDIQRGAVDKEKTGVFTGGYAINPVNQARIPIWIADYVLMSY
                     GTGAIMAVPAHDERDFAFAKKYDLPIIPVIQPEGQGEAGVLESAFVGAGVMINSGMLD
                     GTKVNTEKGRKNPSIAKVIDWLEGQGIGKESVNYRYRDWLISRQRYWGAPIPMVNCEI
                     HGWNPVPDEQLPVELPEDVEWKPTGESPLKLHPTWKQTTCPVCNGPATRETDTMDTFM
                     CSSWYHLRYLSPTYDQGPFDPAEYDYWMPVDTYTGGIEHATMHLLYTRFFHKALRDIG
                     IVKGNEPMTQLRNQGMVLGEDNEKMSKSRGNVIAPDVLVERYGADTVRAYIMFFARWE
                     MGAPWDSQGIEGAVRWVKRVWTLFTDPTPPTTASEETKKNLRRKVHQTLRRVTRNFEQ
                     FEFNTIVSSLMELMNEMYKAREAGAVGTEEWKEAQDIYLRMMAPVTPHVAEELWSQLG
                     KPYSVHTQQWPAVDEAAAKEDSIELPVQINGKVRDRIIVAAEASEEEVKAAALASEVV
                     QKYLEGKEPRKVIVAKGKLVSVVL"
     gene            complement(8354..8429)
                     /locus_tag="PLE39_15200"
     tRNA            complement(8354..8429)
                     /locus_tag="PLE39_15200"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8394..8396),aa:Ala,seq:ggc)
     gene            complement(8452..8524)
                     /locus_tag="PLE39_15205"
     tRNA            complement(8452..8524)
                     /locus_tag="PLE39_15205"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8489..8491),aa:Ala,seq:cgc)
     gene            complement(8590..9900)
                     /locus_tag="PLE39_15210"
     CDS             complement(8590..9900)
                     /locus_tag="PLE39_15210"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE69728.1"
                     /translation="MQIGQVGLEFDLKKKYTVSMPTLSKTGQLKELEGRHSLLLRLVE
                     LSVTLNSTLDLDVLLQLITATATELLECEASSILLYDEKNPRLFFAAATGSDPAKLAE
                     IPVPIDNSLAGTIFRTNKALILNNAEHDPRHYAPVSEHIKFKTNTLLGVPMPIKDRTM
                     GVLEAVNKRNGAFNDGDVATLSVIASHAAIAINNARMLRTTQQALEKVKVTNQIKSNF
                     LALASHELRTPLGIIIGYATFLQEGAKGESSDHANQVLGAAAQMRALLDQMTNLTLLQ
                     TDEMQMRPIRISIQDVLNFAVDEIKYFAARRDLSLVFDFPETPIYVNVDAEKTALAFV
                     NLLNNAIRFSPEGSEIVIGAVEQGKKVLASVRDHGIGIPSDKLQKIFEEFYQIEPPNT
                     RRHGGLGIGLTIAKGLIEAQDGKLWAESDGLEQGSTFKVLLSTT"
     gene            9979..11985
                     /locus_tag="PLE39_15215"
     CDS             9979..11985
                     /locus_tag="PLE39_15215"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNE69729.1"
                     /translation="MSRFTRLHAPILGTLVFQGLLLFWAVRTDGRDLPHIATLFSLVI
                     SLLILITGLLPAIVRPQESHTLVACFAVSFAFLLFIPFEISDMPFSQPGAPLIQFIPP
                     LILARLINGAVILPMAIHMSARFPRRNSIPTHAIVGAYIVSIIVVSLFLAASYPWQRL
                     TMLIFTMGWFLVVIIFFFMNLLHAARDPINLQDAQRARIVFFSIAVAEIPLLMRPFSI
                     ALNLNALPYNVVLFFQLFVPLGISYAVLRHDLFGIDRVLRRTLAYGTVSLLLLTLYLA
                     LTTGMTALFTDSITSRPIAPLVSLFIAAILFEPTRKFVQTWLDRILYPDRLRFQNAVQ
                     EMQNSLARANRREQIIHLLNEVFPAQIGAEWGALKLFPEPDVPPPHLTPGWNTRLIAG
                     SVSFGGYWLGMRKAGPLYDSDERARLSALGQQAAFALAYANAYENLYALNQTLEERVK
                     EQTQLALENQKSVAAYEERQKIARDLHDSVTQSLFGIHLMARGLAAKSTPEMREDLVA
                     LETQARETLREMRLLLNQLRNASAEENVDLTEAIQGACEAFAQRSGPEGGPLLSITLE
                     MPKGVILRKSIADEALWVIREALQNIVKHSGSRDATVTVQKDTMLRGTIRDFGKGFDV
                     NDLPAGHYGLRGMRERVLALGGELKIEFNEGTTVSFALPLPTAK"
BASE COUNT         2716 a         3270 c         2920 g         3087 t
ORIGIN      
        1 ttcgtacaaa gcaaattcac ctgccacata catgcctgcc cgctcataaa ggcgggtggc
       61 tccggttagg ttggcggaat caacacctaa accaatgatt gttgtaccgc gtttgtaaaa
      121 ctcgccaaaa gtttgacgca ttagggctat accaagactt tttccacgcc aagggcgacg
      181 gacggcaatt gttccaatcc agccaattcc tttgcggaaa cggttctggg aaaagcctgc
      241 tacttcattt ccatcccacg caatcatcca tagtgtaggg tcaaaatttg ggttgccaaa
      301 ttttgcatga gaccattctt gaaaggtacg gtcatggctg ccccaatggt cacggaatgc
      361 ttcgttgtct gcctgccaga ctgcaaccgc atgttcgtct ttgatgaatg gccgcagttc
      421 aataccagca ggaaggttta aagcgggttg ggcttcctgc aatttgattt ccatgcgcca
      481 aaaataacga acaagagaat atccattgtt tttaagtaag ttatgacccg cttcatcttt
      541 gttgttgatt aaactttgaa tagtcacctg tacattaggt acagccagtt ctatttcagc
      601 ccgcgcacgc tcggctattt tttccagtaa ggatgaacca acgcctaatc ctctaaattc
      661 tgggtggaca catccgtctg ctttaagttt atagtgacca ctctcattat agaattcttc
      721 actaccaacc aaacgtccat cttgagtttc tacaacaaaa acatcacgct ccacattgaa
      781 gccttcgttt tcccactcgt ttgccagttc ttcagcggta agcacaaaaa gcgaatcgcc
      841 ctccatttcc gcaacatcat gattgagttt tgctaccgca ttgacatctg tccaacgggc
      901 acggcgtagc gttaggcttg ggtcaagata aatcttttca gtcataacaa tctccgctca
      961 ccttcagttt acctataaag ttcgtataaa ttttgcaaga gctgaccaac agaccagcac
     1021 acaaacgggg tgccctttat aaatattggg caaaatcaac cgcctcccta tcttcatcaa
     1081 ttttcaacag gtcttttagt atcgcagtat gcccctgccc gccaatggcc aatactcttt
     1141 ctccaggggc ggcatgcttt tgaatattgg catacattct gaagtttcta tgccaccagc
     1201 tcgccgccgc atcagcgcct tcaaagttat ctcctgcgcc gacatggttc gtgatcaagt
     1261 atatatactt gttgtgcctg tctgtttcct ccgcattggc atccatcaac aactcagcca
     1321 gagacaatgt ttcatgtgcc cgctttgtcg tctcgccgat ttgttcgatc agggcatcaa
     1381 accgagactt tgtttccgcg tcatgtgtat tgatgtattc aaacatgggc ccggcattcc
     1441 aaccaatggt cctttcatca aaactgtaca cggttttcag gctggatttt tttgcaatac
     1501 ggaaaccgag ttgataaatc tcattggagg gcagggtaaa actgcctgtg agatactggt
     1561 catatttctc ttgaactgcc ttttcatcta cagggtcaaa ctcaagcagc acggcagtgg
     1621 gcatgaattt actgatctga tctgtaaaac gctcaagata ttcctgattt tccgatgtca
     1681 taacattgag gtgctccgtt ttaactagat cctgcatggg atccataaaa tggaacaccc
     1741 ccaacaacat aacctttgcc ttttgttcag aaacaacacc gctcatatta ttcgcatcct
     1801 tgataaaaag ggccttattt tttgaaacat ttgatccaat gggcagccaa agacaaaacg
     1861 acagttacac gaccttcttc ctcaagatcc tgttccaatc catcatcgtc ttcaaatgct
     1921 ccatcggcac ctgcccctcg ggcacaaagc cgacgaagga acggttatag gtgtcttctg
     1981 ggccatcatc aaggtgccag gagagcggca ttctaaaagt gccaacggaa gacgtacctt
     2041 tatcatcatc gggcagggcg gtttcatagg tgatgaccat tgtcccatcg gcggtttcag
     2101 ccacctcagc caccgtcgct tcgtggatcc agcgcagcca ttggtgctgt tgagatttga
     2161 ccttggtctc agggttgatc aatgtccgcg agcgggtcac cgtctcaccc ttttgatatt
     2221 cccacggcgt agaagtagcc ttgggatcgg atttgtcgcc ttttttcttt cccatgtctg
     2281 gcagcaaccc cacacccgat ttggttttca cttccttggc cacctgccag gcattcggct
     2341 ctttatctac aaagcgcagc cagaaaatac tggcaccggg cacataatct gctgcagatg
     2401 tgggccgtgt atagaacgga cggggatcac cgggcaattt gccctcggtc tcattattga
     2461 aataccattt cggcttgggg gtcaggccgc cggggggcaa atgtccgcgc aagtgctgac
     2521 aatgctcggc cgtttcgttg cagaccgcac ggctgacaat taactcatga gagcgtccat
     2581 gctcttcacg ccatgcagat gaatcataat tttcccaggc atccaacaca ttgcgaatat
     2641 cgttgtatgt ctgtgaataa cgcgcagctt cctcttcagg ggttagtttc tcccaattcg
     2701 gatctgtaac atggttcaca cgcagcggcg tgagcttgac gatttccttc cacgcccagg
     2761 cagggaaggt ggatttcata tcttcgagcg ccttgagcgc ttcctctgta ctcaatgaat
     2821 ttatttcgta attcaactct tcagcctgca aagcggtcaa accgttgcgg cgggtcttgg
     2881 gtccgctggc tttcacgctg agcatgtgca gggccacttt atctttccat tctttttctg
     2941 aggcacgagc cagcttgggg atgttggacc ctgttccagt ttcgtagacc gcgatctttt
     3001 cggtgcagag tttctccacg gcggcatcat ccagtttgcg aatctcgctc ccggcgttcg
     3061 atccacgcaa acgcatcagc agcaacttgg ggtctgccca tgaattatgc gcactcgcgt
     3121 aacgcatccc cgaaaaatgg acgaccatgt attgcagcca atgcgggtaa cggttcggtt
     3181 ccttcttgaa gcgctgataa atttcctcca gcagttcata ttgattcttc ttttcgaggg
     3241 aggtcttgta ttcttccacc ttccaacggg caatatcctg aaccgtgatc ggcgcttcag
     3301 gcttatagtg gactaaaaac tccgattctg acgtactgaa agctggatca gccttcgact
     3361 gtttgtacca cgcgagtttt ctttgctcga ccttatcgta ggtcttgaga aaagcacgca
     3421 cctgatccag ttcctgctcg gccatgggca gggtgatgcc ttccatcgtt tcaagttcct
     3481 gcgcttcgat ggggcgcttc gggtgggcgg ggtccatatt ttcaacgcgc cgccgcttgg
     3541 atttggccgc ctctttctgc atgttgcgat gattgaccca atattgaagt tggccctcca
     3601 caaagctccg ctcaccacgg atatccttgg gccagccttc gataaaaatg gcatgaaaat
     3661 tattgatctc ggtcaattcg gcttcgttaa taggcttgta cttcaggtaa tatccccgca
     3721 catctttcaa cataaaatag gaacgcaaag cagagtaaga tttgtaggcg gcaaccaggg
     3781 caaactcttg tgccgtgatg tacgcatcca cttccgtttt gagtgtggaa atgtccttgt
     3841 ccttgtattg tttccacaaa tcaatttcca tgtaatagac cagaaaatcg cgcaatttcg
     3901 gttcgcgcat tgggtaaatg gtcttgcaca aaacagtatt ctgccattgg aagtacagcg
     3961 gctcagccat gagtggcctc cttgagaatt ggacgttttg agaaaatgat gcttcatttt
     4021 attccaaacc tgcccaaaaa acgaaacgga tttcctgctc ttgcaggaga gagtcaatga
     4081 aaatatactt gcctgcattc aaacgcacac ccccacagaa aagagacggt aacgatggaa
     4141 gatagagagt tatatggaaa gagcgcgatc tttggcatgg ggcttggagc ccttctaggg
     4201 ttgatactgg gcggagccac aggcgccctc agcctgacat ggggtggaat tcaactggct
     4261 gggcttgttg ggctggcagc cggggcagtg accggggcac tgactggact gatctcggca
     4321 cgggttgcag gccgcacggg gggtgtcagc tttggggctt acggcggcat ggcatttggc
     4381 ggattccttg gcctgatctt gggagccttc atccctgctt cgttccgcga aagcatcgcc
     4441 accgaccaaa tgccttacct caccgtcctc gcaaatggga catttgaaat ggcgatcctg
     4501 gtcagttttt tgctggcctg ccttggaacc gctgttggcg catgggtggc gggacgtaat
     4561 ttcattccac gtgaccagcg cgaaggatga caggcatgga taaaactttt atcaaagacc
     4621 tgttggtgcg cggcatcatc ggcatccgtg attgggagcg ggtgacaccg caggatatcc
     4681 tgatcaatgt gacggtattc agcgacacac gccttgccgc gcaaaccgat gacattgccc
     4741 actgtgtgga ttacagcgca ttggcaaaaa aggttcaggc gcatgctgaa acttccgcac
     4801 gccttacggt ggaggcactg gccaacgacc tcgccgaaat ttgcctgaag gagaacggcg
     4861 cccaaaaagt gatcgttcgc gtggaaaagc cctcggcagt gcggttcgcc aaatcggttg
     4921 gcgtggagat cgaacgcagc cgcaacgaat aaaaacggga gaacctaaaa aacagaatgc
     4981 ccccgaaagt cactcgactc tcggaggcat tctgttttaa tccaaccccg ggcgatggcc
     5041 tgggtaattt actctttcag caaaccagtc aactacaaaa ccacgctgac caatttacct
     5101 tttgccacga tcaccttgcg aggctctttg ccttccagat atttctgaac cacctcgctc
     5161 gccaaagcag ccgctttaac ctcttcctcg ctggcttctg cggcaacgat gatgcggtct
     5221 ctcactttcc cgttgatctg aacgggcaat tcgatggaat cttccttggc agcagcctca
     5281 tccacggcgg gccattgctg ggtatggaca gaatacggct tgcccaactg cgaccacaat
     5341 tcttcggcaa catgcggggt gacgggagcc atcattctca agtaaatatc ctgggcttcc
     5401 ttccactctt ccgtgccaac cgcgcctgct tcgcgcgcct tgtacatttc gttcatcaac
     5461 tccatcaacg atgacacgat cgtgttgaat tcaaactgct caaagttacg tgtcacacgg
     5521 cgcagggttt ggtgaacctt tcggcgcaga ttctttttcg tttcttcaga ggcagtcgtc
     5581 ggcggagttg ggtcggtgaa caaagtccac acacgcttta cccaacggac cgcgccttcg
     5641 ataccttgcg agtcccaggg tgcgcccatt tcccagcgtg caaagaacat gatgtaggca
     5701 cgaaccgtgt ctgcgccata acgttccacc aacacatcgg gagcgatcac gttgccgcgg
     5761 ctcttcgaca tcttctcgtt atcttcgccc aacaccatgc cctgattgcg taactgcgtc
     5821 atcggctcat tgcctttgac aatgccaata tcgcgcaggg ctttgtggaa gaaacgcgta
     5881 tacaacaggt gcatggtggc atgttcaata ccgcctgtgt acgtatccac gggcatccag
     5941 taatcatatt cagcaggatc gaacggaccc tgatcgtagg tcggcgacag gtaacgcaag
     6001 tgataccacg acgaacacat gaacgtatcc atcgtatcgg tctcacgcgt agcggggcca
     6061 ttgcacacgg ggcaggtggt ttgcttccag gtgggatgca acttcaacgg agattcgccc
     6121 gtcggcttcc attcaacatc ttcgggcagt tcaacgggca actgctcatc aggcacggga
     6181 ttccatccat gaatttcgca attgaccatc gggatcggcg cgccccaata acgctggcgg
     6241 ctgatcaacc aatcgcgata acgatagttg accgattctt ttccaatgcc ctgcccttcc
     6301 agccaatcga tcactttcgc aatgctggga ttcttgcgtc ccttttcggt gttgactttc
     6361 gtgccgtcca acatgcccga attgatcatg accccagccc caacaaaagc ggactctaac
     6421 acacccgctt cgccctgacc ctcaggctgg atcactggaa taatgggcag gtcatacttt
     6481 ttggcaaagg caaagtcacg ctcatcatgc gcagggacag ccatgatcgc acccgtgccg
     6541 taagacatca aaacataatc cgcgatccag atcggaatgc gggcctgatt aacagggttg
     6601 atggcatagc cgccagtgaa cacacctgtc ttttccttgt ccactgcacc gcgctgaata
     6661 tcggtctgac gggcagcctg cagcttgtat tcattgaccg tggccttgtt ctcttccgtg
     6721 gtgatctttt ccaccaatgg atgttcaggc gagaagacca tgaaggttgc gccccacaac
     6781 gtatcggggc gggtggtgaa gatctcgaca tcatcaccct gctcggtctt gaaaataacc
     6841 gaagcgcctt cactgcgatc gatccaattg gtttgcaggg tcttcacgcg ctctggccag
     6901 tcaatgcctt cgaagctcaa taactcatcg gcgtatttgg tcgccttgaa gaaccactga
     6961 tccagatcct ttttgatcac gggcgtgccg cagcgttcgc agtgacggtc atcgccccac
     7021 acctgctcac gagccagtgt ggtgttgcat ttcgggcacc aatccaccgc agacattttg
     7081 cggtaggcca gcccagcctt atacaactga atgaagaacc attctgtcca tttgtagtag
     7141 gcggggtcgg cagaaacggc ttcacgcttc caatcgaaca tcgcacccat cgaacgcatc
     7201 tgcttgcgca ttcgctcaat gttttggaaa gtgcgcttca tggggtggac accgtctttg
     7261 atggccgcat tttcggcggg caggccgaag gcatcaaaac ccatcgggaa cagcacgttg
     7321 tatcccttca tacgcatgta acgcgcccgc gcatcagacg gggtcatcgc ataccaatga
     7381 ccgatgtgca agtcaccact ggggtacggc agcatggtca acgcgtagtg tttcggcttg
     7441 gaagggtcaa tcaccgaacg gtacaactga tccgcatccc attgatcctg ccatttcttc
     7501 tcaatattct ttgggttgta ggatttgtcc tgctggacgg gtgcagagtc agccgccttg
     7561 actggcacaa ttgccttcgg tttttctgtc acaggcacaa ctgcctttgg cttttccttt
     7621 gcaaccacca ccgccatttt ttgggcgggc acgattgcct tggtggtttt ctttgctgcc
     7681 ttgggggcag ccttcttgac aggtgccttt tttgcagcaa cagacttttt ggtcacggct
     7741 tttttggcaa caggcttgct tttaaccacg gttttcttcg ccgctggttt agccttggtc
     7801 gcagccttct ttacaacctt ttttgttttt gaagcaaccg ccttggcggt ggtcttcttt
     7861 tttactgttg ttttcttttt ctcagccatt cttacctcat caaataaata tcaggtttca
     7921 aggacacagg cactaatgga tcattagccc tgtttgttca ggcaattcgg aaggaacgga
     7981 aggcctgcaa gcaaaggtcc ctgcttccaa aagataacta tatttttcat atcagcctcc
     8041 tttccatgat taaacaaaaa acccttcatc cacatcaggg acgaagagtt tactccgcgg
     8101 taccacccag attcgattga aagttgatat caatcgctct ttatcgctat aacgggccta
     8161 cccgtcgctg actactttat ttcaccagca aagtcctctt acgagataac aggcgagttc
     8221 ggttctggag tgctccggtg acactggtgc tgggctttca cctgccccaa ctcgctgacg
     8281 gatatgacct actactcctg ttttgacttt ttattattgt aggtcacgct tacaacgtga
     8341 cctacgcttt tgttggtgga cccagaggga ttcgaaccct cgaccttctc aatgccattg
     8401 agacgcgctc ccaactgcgc tatgggccct tatgaatgtt ttctgtagca gtggacctga
     8461 ggggattcga acccctggcc tcctcagtgc gattgaggcg cgctcccaac tgcgctacag
     8521 gcccgttcgt aaggcgaagc gtattctacc tgaggcattt gggaatgtca atgtggaaac
     8581 gggggaaatt tatgttgtcg agagcaaaac cttgaaagtg gaaccctgct ccagaccatc
     8641 tgattcggcc catagttttc catcctgagc ttcgatcagg cctttggcaa tggtcaggcc
     8701 gatccccagt ccgccatgac gacgggtatt gggcggttcg atctgataaa actcttcgaa
     8761 tatcttttgc agtttgtcag atgggatccc gatcccatgg tcgcgcaccg acgccaaaac
     8821 cttcttcccc tgttcaaccg caccgatgac gatctcactg ccttcgggcg aaaaccgaat
     8881 cgcattgttg agcaggttga cgaaagccag cgccgtcttt tcggcatcca cattgacata
     8941 aatgggagtc tctggaaaat cgaagaccag gctcaggtca cgccgtgccg cgaaatattt
     9001 gatttcatcc acagcgaaat tcagcacatc ctgaatcgag atgcggatcg ggcgcatttg
     9061 catttcatcg gtttgcagca gggtcagatt ggtcatctgg tcgagcaggg cacgcatctg
     9121 tgcagcggca cccaacacct ggttggcatg gtctgaagat tctcccttgg caccttcctg
     9181 caaaaaggtg gcatacccga tgatgattcc cagcggggtg cgcaactcat gcgaagccag
     9241 cgcaaggaaa ttgctcttaa tctggttggt gactttgacc ttctccaaag cctgctgggt
     9301 ggtgcgcaac attctcgcgt tgttgatggc gatcgcagca tgggaggcga tgaccgacaa
     9361 ggtagccaca tcgccatcgt tgaaggcgcc attgcgcttg ttgaccgcct cgagcacacc
     9421 cattgtccga tctttgatgg gcatgggcac accgagcagg gtgttcgtct taaatttaat
     9481 gtgttcagaa acgggggcgt agtggcgcgg atcatgctcc gcattgttca ggatgagtgc
     9541 cttgttcgtg cggaaaatgg tgcctgccag gctgttatca atggggacgg ggatttccgc
     9601 caattttgca ggatcagacc ccgtggctgc tgcaaaaaaa aggcgcggat ttttttcatc
     9661 gtaaagcaaa atggatgatg cttcgcactc cagcaattct gtggcggtgg cagtgatcaa
     9721 ctgaagcaga acatcaagat caagcgtgga attcagcgtg acgcttaatt ccaccaaacg
     9781 caacaacaaa ctatggcgac cttcgagttc tttaagttgg cctgtcttcg aaagtgttgg
     9841 catggagaca gtgtacttct ttttcaaatc gaattcaagc ccaacttgtc cgatttgcat
     9901 gtcaaaaaca tggcaagtgg aggtatgccc attctcccac ttgacccact cccccgcacc
     9961 ccctacaatt tgaacagaat gtcccgtttc acacgccttc atgcgcccat tttaggaacc
    10021 cttgtatttc aggggcttct cctgttttgg gcggtgcgca ccgatggccg agacctgccc
    10081 cacatcgcca ccttgttttc gctggtcatt tcacttttga tcctgatcac tggccttttg
    10141 cctgccatcg tccgcccgca ggaaagccat acccttgtgg catgctttgc cgtttcattc
    10201 gcctttctgc ttttcatccc atttgaaatc agcgacatgc ctttttccca accaggcgcg
    10261 ccgctgatcc aatttatacc gccgttgatt cttgcgcgtc tcatcaatgg agccgtcatt
    10321 ttgccgatgg caattcacat gagtgcgcgt tttccgaggc gcaattccat ccccacacat
    10381 gcgatcgtgg gcgcgtacat tgtttcgatc attgtggtat ccctgttttt ggcggcatcc
    10441 tacccgtggc aacggttgac catgctgatc ttcacaatgg gatggtttct ggtcgtgatc
    10501 attttcttct tcatgaattt gctccatgca gccagagacc ccatcaacct gcaagatgcc
    10561 caacgcgccc gcatcgtttt cttcagcatt gctgttgcag agattccgct tttgatgcgc
    10621 cccttttcca tcgccctcaa cctcaacgcc ctgccctaca atgtggtgtt gttcttccag
    10681 ctttttgtgc ctttgggaat ttcctacgcc gtattgcgac acgacctctt cggcattgac
    10741 cgagtcctca ggcggactct ggcctatgga acggtctcgc ttcttctctt aactttgtat
    10801 ctggctctga ccacaggcat gaccgccctt tttacagatt cgatcacctc gcggccgatc
    10861 gcgcctttgg tcagtttgtt catcgccgcc attctcttcg agccgactcg caaattcgtc
    10921 caaacctggc tggaccgaat cctctacccc gacaggctgc gattccaaaa cgcggtgcag
    10981 gagatgcaaa attcgctggc acgtgccaac cgccgcgagc agatcatcca ccttctaaat
    11041 gaagtcttcc ccgcacagat cggagcggaa tggggcgcgc tgaaattatt ccccgaaccc
    11101 gatgtgccgc ccccacacct gaccccaggc tggaacacac gcctgatcgc agggagtgtt
    11161 tcttttggcg gctactggct gggcatgcgc aaagcgggtc cgctctatga ttcggatgaa
    11221 cgcgcacgct tgagcgcatt ggggcagcag gctgcttttg cactggcgta cgcaaatgcc
    11281 tatgaaaatt tgtacgcgtt gaatcaaaca ctggaagaac gcgtaaaaga gcagacgcaa
    11341 ctcgcgcttg agaatcaaaa atcggtggcg gcgtatgaag aacggcagaa gatcgcccgt
    11401 gacctgcacg actcggtgac acaaagcctg ttcggcatcc acctcatggc ccgcggactg
    11461 gcggccaaat ccacgcctga gatgagggag gatctggttg cgctggagac tcaggcacgt
    11521 gagacactgc gggaaatgcg cctgctgctt aatcaattgc gcaatgcttc agctgaagaa
    11581 aatgtcgatc tgacagaagc cattcaaggg gcgtgcgaag cgttcgctca gcgaagcgga
    11641 cctgaaggcg ggccgctcct ttccataacg ctggaaatgc caaagggagt catcctccga
    11701 aaaagcattg ccgatgaggc cttgtgggtg attcgcgagg cgctgcaaaa tattgtcaaa
    11761 catagcggca gccgcgacgc aaccgtgacc gtgcaaaaag ataccatgct gcgcggaacg
    11821 attcgggatt ttggaaaagg attcgacgtg aacgacctgc ctgcggggca ttatggcctg
    11881 cgcggaatgc gcgaacgtgt gctggccctc ggcggcgaat tgaaaataga gttcaacgaa
    11941 ggtacgaccg tttcgttcgc tttgccgctg ccaacagcaa aataaaattc caa
//
LOCUS       DAOQJC010000119        11588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2805_length_11588_cov_8.853637, whole genome shotgun sequence.
ACCESSION   DAOQJC010000119 DAOQJC010000000
VERSION     DAOQJC010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11588
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2805_length_11588_cov_8.853637"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2224)
                     /locus_tag="PLE39_15220"
     CDS             complement(<1..2224)
                     /locus_tag="PLE39_15220"
                     /inference="COORDINATES: protein motif:HMM:NF024295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HNE69730.1"
                     /translation="MKSFSILFRYLLIFMLVAGTFTNVPVASAIPSAASGPALSVNAA
                     ADVHPISPYIYGMNFATEAIAADLNLPVRRWGGNSTTRYNWQLDVHNTGADWYYENIP
                     EDNAHPELLPNGSESDEFVEQDRRTNTETIMTVPLIGWTPKARREDHPYDCAFKVSKY
                     GPQDSTDYWDPDCGNGWHNGVQLTGNNPTDTSVAITSSFVTAWINHLKGNYGTAANGG
                     VMFYNLDNEPMLWNSTHYDVHPNPVTYDEMRDKTYAYAAAIKAADSSAQTLGPVEWGW
                     CAYFYSAADGCSPGADRAAHGNVDFVPWYLQQMKAYEQAHGVRILDYLDLHVYPQVDG
                     VYSDALGSAAVQSARLRSTRQLWDPGYVHEGWIGQPVYLIPRMEDWVANNYPGTKLAI
                     TEYNWGALGYMNGALAQADILGIFGREGVDLATLWGTPDDFNAPGIFAFRMYRNYDGA
                     GGTFGDTSVQSSSADQSQLAIYAAKRSTSGTLTLMVINKTASTITSDLTLSGFQPVDT
                     AQVYRYSSANLGAIVKQADQAVTSSGFTASYPANSITLIVIPSSVSQTTFVDVPADHP
                     LYAYIQALYDAGYTAGCSTDPMMYCPDTILDRAQSAVFMLRGQMGSGYTPPAAPWNTF
                     GDDWTGFEWAEPWAEGMYQEGLTTGCQTSPLLYCPANQLPRVEASIFGLRMKYGVNYT
                     PPAASGALFADFPPSDPSYWGIDWAEQAYLDGLLPACGTDSATGKPMFCPSQLVDRGW
                     G"
     gene            complement(2396..5284)
                     /locus_tag="PLE39_15225"
     CDS             complement(2396..5284)
                     /locus_tag="PLE39_15225"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNE69731.1"
                     /translation="MTKRNRMFLSIASSLMLLAVALGLWGRLGPMSTQVTQALSSNVQ
                     HVETDEKEADHPEEAREYRALAWRDENGNIPADGLFTAAQQINEMRAQQGNNPQAITS
                     AGWTWIGPGNIGGRVRSIIIHPTDTNIMWAGSVTGGIWKSTDGGASWNILTDFMSNMA
                     VTTMVIDPSNPNILYAGTGEGFTGNREISEPLGGGVFKTTDGGSTWTQLSSTNNSNWY
                     FVNRLAIHPSNPQILLAATYTGLWRSTDGGSSWSSVLTGYMADVNFNPSDGNKAVAGN
                     QFSGQAYYSTTGGASWGSTNTGGNGRVEVAYAPSSPSTVYASVDNLGGQLWKSVDGGV
                     SYALVNSGNDFLSGQGWYDNALWVDPTNPNTLVVGGVDLYRSTNGGSTLTQISEWWNS
                     PQSAHADHHVIVEKPGFNGTSNTTVYFGNDGGVYRANNVYTVVGTTGWTELNNNLGIT
                     QFYGAAGNATTGKIIGGTQDNGTLIYTPAGGTEGWTTTFGGDGGWSAADPTDDNYLYG
                     EYIYAQIHRSVNGGVYSDPIWGEYWDGSSYQYKNPPYIITDTYNSNANFISPFILDPN
                     DSNRMLVGAASLWRSNDVKAPNTTTTGPSWYVIKSSTGYNITAIAVAPGNSDIVWVGH
                     ENGDVFKTTNGTATNPTWTRADTGSPALPNRYVTRITIDKNDNNKAYVTFGGFSTDNV
                     WATTNGGSTWADITGSGATGLPSLPVRSLVIHPNDSNWLYIGTELGLFASSDGGATWG
                     LPHDGPTNTPVDELFWMGTKLVAATYGRGLFTQEASNTLFADVPADYWALSFIERLYN
                     AGITGGCGTNPLIYCPEAPVTRAQMAIFILRGIHGSAYVPPAATGTVFTDVPAGSFAA
                     DWIEQLAAEGITGGCGNGSYCPDAYITRAQMAIFLLRGEHGSAYTPPAATGTVFGDVP
                     VGAFAADWIEQLAAEGVTSGCGGGNYCPDANVTRAEMAVFLVRTFSLP"
     gene            complement(5379..6224)
                     /locus_tag="PLE39_15230"
     CDS             complement(5379..6224)
                     /locus_tag="PLE39_15230"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNE69732.1"
                     /translation="MQFNSQRRMFHSIAFHAVSILISALFILPLVWMASASLRQPGLP
                     PPRTVEWWPEPFAWSNYTRIFEMLPMGRYVLNSLLVAGLAVPITLVTASLAGFAMSQV
                     NRRWRENLLGLSVVLLVVPTVAVWVARFILFRTVGLFNSYLALLAPSVMGSSPLFVLL
                     FFWSFRRIPRELYDAARMDGAGPLEIWWRVALPLSNATMIVVGILTFLLYWGDFTTPL
                     FYLKSQTLYTLPVGLRQLQELDRTNWPLLLAGSMVMTLPSVLLFGIVQRFFLPENRLA
                     GIYGQ"
     gene            complement(6214..7110)
                     /locus_tag="PLE39_15235"
     CDS             complement(6214..7110)
                     /locus_tag="PLE39_15235"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNE69733.1"
                     /translation="MNRTYTASSRTQLLLMLTPYLLGAVVLVGLPALLSLLLAFLRYD
                     GISAPVWVGGFNFQLVLANPVDLLFPISLYNTLYYVILAVPVRVLSALALALLLNRRG
                     KGVGLYRAVVYLPTIIPDVAYALIWMWIFNPLYGPLNLILEGLGLPTPGWLADAQYAK
                     MVFVVMTAFQIGEGFVVLLAGLQNIPPEYYAAAAVDGGNFWQQFRYITLPLLAPWLLL
                     LTLRDVILSSQNIFTANFIMTNGDPYYSTLFLPLLIYEEAFDRMHFGPGSAMTVLLGL
                     VTAALIGFILSIVGSWGETDAI"
     gene            complement(7103..8362)
                     /locus_tag="PLE39_15240"
     CDS             complement(7103..8362)
                     /locus_tag="PLE39_15240"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNE69734.1"
                     /translation="MRSRLALLFLLVIFLFTTACGQQGPRPVSFMVFGEPAELKAYQD
                     LVAAFEKQHPEIKVELIHIPSQGDYRKRLAADLVGGEPADVVLINYRRFSGLALKNAF
                     EPLGPYLEKSESIAYDDFYALSMRAFSLNDQVVCIPQNISSLVAYYNKDLFDAAGIPH
                     PDAGWTWDDFISASKALTLDVDSDGRVDQYGAGVEPTLIRVAPFVWQNGGKVSINNAL
                     TLAEPLDLEAVQWFVDWQVTHHIVPNAEEEKAESSESRFINGRVAIYFNSRRLVPTFR
                     EITAFDWDVAPLPLGRSDATILHSDAYCLTAASQHKEDAWTFIEFANSAAGQSLIAQS
                     GRTVPSLIQVAESDAFLDPNAKPEHSRVFLDVIPFTLRLPQQANWADIEEICDEELQR
                     AFFGEVTALEAMQSAVERALPLFTENE"
     gene            complement(8522..8989)
                     /locus_tag="PLE39_15245"
     CDS             complement(8522..8989)
                     /locus_tag="PLE39_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69735.1"
                     /translation="MPRLKKYLIIACLLIVTLFNQSCATPATPTAAFTPVIVMTEEPS
                     LEMPTTTPSAPSLTIDPAFQRGQVIPLSVGDVFAISVPPGSSIWKVAYAAEVVEPLTP
                     ADKMTEPGSQGWLFRAVAAGQTDIRLTAPAPACAKPPCPPAAPMVFVFTVDVK"
     gene            complement(9218..11089)
                     /locus_tag="PLE39_15250"
     CDS             complement(9218..11089)
                     /locus_tag="PLE39_15250"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNE69736.1"
                     /translation="MSVVFKKMFFACVVAVMVLTTGAWQNSQDVLAASPRQSAPEAPL
                     FSSLTWADLGSVSRSITTSVRGDAITLSGKGYAAAERFQGGVSQDVADYYSNAQLAAS
                     GWTSYDAYDTAEGTHKVFYHDAGYYFAVDFLTCADDSSSTCVTVWMSEKTAPSPAAQF
                     TGFAPDPFATTFSKTSPASGTINLDPKNLTLTWGTYSPTPDKYSYCVKIGSACDDNDP
                     NWTGTYLNTSVKLTNLEYDKIYYWQIKAITCATCIPKKFVYANDAKWWTFTTKPQSAA
                     GVVILGNVGTPSATLTYVVSGTTKTVSSDGSGGYSITVPSGWTGTITPSKVGFYFTPI
                     NASFSNLTAAQTIQNFTATPTGSGTPEISGNAGVAGAILSYSDGGQAKTAIADSTGAY
                     KFTVSANWTGTVTPSKDNFYFNPAYRSYTNVTAHQTGQNYTISIFYDVPSTYWAFNYI
                     ERLYAAGITGGCSSSPLMYCPEGTVTRAEMSVFLLRGIHGSAYVPPAVGSSTGFNDVP
                     ASHWAAAWIKQFATEGITGGCGTSLYCPEGKASNAEMAVFLLRSKHGAAYTPPAVGGS
                     TGFSDVPTSYWAAAWIKQLAAEGISTGCGNGKFCPDDAVTRASMAVLMVKTFSLPKQ"
BASE COUNT         2885 a         3030 c         3176 g         2497 t
ORIGIN      
        1 caccccagcc gcgatccacc agttgacttg ggcagaacat cggcttgcct gttgcagaat
       61 ctgtgccgca ggcgggcagc aatccatcca gataggcttg ttcagcccaa tcaatacccc
      121 agtagctcgg gtcactgggc gggaagtctg cgaacaaagc tccacttgca gcgggcggag
      181 tgtaattcac gccatacttc atgcgcagac caaagatgct ggcctccaca cgcggaagtt
      241 ggttggcggg gcaatacaat aatgggctcg tttggcagcc tgtggtcaac ccttcctgat
      301 acatgccttc cgcccacggc tcggcccatt cgaaccctgt ccaatcatca ccgaaggtat
      361 tccacggcgc ggcaggcggg gtgtagcctg agcccatttg tccacgaagc atgaacacgg
      421 cagattgcgc gcgatctaaa atcgtatcgg ggcagtacat catcgggtcg gtggagcagc
      481 ctgcggtgta gcctgcatcg tacagcgcct gaatgtaagc atacaaagga tgatccgcgg
      541 ggacatccac aaaagtggtt tgtgaaacag aggaagggat cacgatcaac gtgatcgaat
      601 tcgcagggta gctggctgtg aatccgctgg aggtgaccgc ctgatccgct tgtttgacaa
      661 tggctcctaa attggcagaa ctatagcgat agacctgtgc agtatccacg ggttgaaatc
      721 ctgacaaggt caggtcgctg gtgatggttg aggcggtctt gttaatgacc atcaaggtca
      781 atgtgccgct cgtgctgcgc ttcgccgcat agatcgccag ttgcgattgg tcggcactgg
      841 aagattgcac gctggtatct ccaaacgtgc cgcccgcgcc atcgtaatta cgatacatgc
      901 gaaatgcaaa aataccgggt gcattgaaat catcgggcgt gccccacaaa gttgccagat
      961 ccacgccttc gcggccaaag atgccgagga tatcagcctg agccagtgcg ccgttcatgt
     1021 atccgagcgc accccagttg tattcagtga tggcgagttt cgtgccgggg taattgttcg
     1081 ccacccaatc ttccatgcgc ggaattaaat acacaggctg accgatccag ccttcatgca
     1141 cataacctgg gtcccaaagt tggcgggtgg aacgcaaacg cgccgattgc acagcggcac
     1201 tgcccaacgc atcggaataa acaccatcca cctgcggata cacatgcagg tcaagataat
     1261 caaggatgcg cacgccatgg gcttgttcat aggctttcat ctgctggagg taccaaggca
     1321 caaaatccac attgccgtgc gcggcgcggt ctgcaccggg ggaacaacca tcagcggcgg
     1381 aataaaaata agcgcaccag ccccactcca ctggtccaag cgtttgcgca gatgaatcgg
     1441 cggctttgat cgcagcagca tacgcataag ttttatcgcg catttcatca taggtgaccg
     1501 ggttgggatg cacatcataa tgagtgctgt tccacagcat cggctcgtta tccagattgt
     1561 agaacatcac gccgccattg gcagccgtgc cataattccc cttcaaatga ttgatccatg
     1621 cagtgacgaa agaagaagtg atcgctacac tggtatcggt ggggttattg cctgtgagtt
     1681 gaacaccgtt gtgccagcca ttgccgcaat cagggtccca ataatctgtg ctgtcctgcg
     1741 gaccatattt actgactttg aatgcgcaat catacggatg atcttccctg cgggccttgg
     1801 gcgtccagcc gatgagggga acggtcatga tcgtctctgt gttggtgcgg cgatcctgtt
     1861 caacgaactc gtctgattca gaaccattgg gcagcaactc cgggtgggca ttatcttcgg
     1921 gaatgttctc gtaataccaa tccgcgccag tgttgtggac atccaattgc cagttgtaac
     1981 gtgtggtcga attcccaccc caacggcgga ctggcagatt caagtcagcg gcaatggctt
     2041 ccgtggcaaa attcattcca tagatatatg ggctgatggg gtgtacatca gccgcagcat
     2101 tcacactcaa tgccgggcct gaggccgctg agggaattgc cgaagcaaca ggcacattgg
     2161 taaacgtgcc cgccaccagc ataaaaatca ataaataacg aaataggatt gagaaacttt
     2221 tcattcgagt ctcctatgca tttttccggg aagggatttg acagatatag tgtacccctc
     2281 cacttaaaat tttgaaacat acatcggtac catcgaggca acggcttgag aaatagaaaa
     2341 cgcaccccga aattgttttc ggggtgcgtc tgcttactgg aacatgtttt tttgattatg
     2401 gcaaactgaa cgtccgtacc aggaagacgg ccatttcagc gcgggtgaca ttggcatcgg
     2461 ggcaatagtt accgccgcca cagccgctcg tcacaccttc agccgcaagc tgctcgatcc
     2521 aatctgcagc aaatgcgccg acggggacat caccaaagac cgtacccgta gccgctggcg
     2581 gagtgtaggc actgccatgc tcgcctctca acaggaagat cgccatttgg gcgcgggtga
     2641 tgtaggcatc gggacagtag cttccattgc cacaaccacc cgtgatgcct tcagccgcaa
     2701 gctgttcgat ccagtctgct gcaaaggacc cggcagggac atcggtaaag accgtaccgg
     2761 tcgctgcagg tggaacatag gcactgccat gaataccacg caaaatgaag atggccattt
     2821 gagcgcgggt gacaggcgct tccgggcaat agatcagcgg attggtacca cagccaccgg
     2881 taatgccggc attgtacaac cgctcaatga acgacaaggc ccaataatcc gcgggaacat
     2941 cggcaaagag cgtgttggag gcttcctgtg tgaacaagcc gcgtccgtat gtcgctgcaa
     3001 ccaattttgt tcccatccag aacagttcgt ccacgggggt gttggtcggg ccatcatgtg
     3061 ggagacccca agtggcgccg ccatctgaac tggcgaaaag accgagttct gtgccgatat
     3121 acagccagtt ggaatcattc gggtggatca caaggctgcg gaccggcaaa ctgggcaaac
     3181 ccgtcgcgcc gctgccagtg atatcagccc aggtggagcc gccattggtc gtggcccaca
     3241 cattatcagt ggagaaacca ccaaacgtga cgtaggcctt gttattgtcg ttcttatcaa
     3301 tggtaatgcg ggtgacataa cggttcggca gggcaggaga acctgtatcc gcacgagtcc
     3361 atgtgggatt ggtggcggtg ccgttggtgg tcttgaacac atcgccattt tcatgcccta
     3421 cccagacgat atcagaatta cccggagcaa ccgcaatggc agtgatattg tagcccgtgg
     3481 atgatttgat cacataccag gatgggcctg ttgtggtggt gttcggcgcc ttgacatcat
     3541 tggaacgcca caacgaagct gcaccaacca gcatgcggtt cgaatcgttc gggtcgagga
     3601 tgaagggcga aatgaaattg gcattcgagt tataggtatc ggtgatgatg tagggcggat
     3661 tcttgtattg gtaggaagag ccatcccaat attcacccca gatcgggtct gagtacacgc
     3721 cgccattcac actgcgatgg atctgtgcat agatatattc gccatacaga tagttatcat
     3781 ctgtcgggtc ggccgccgac cagccgccat caccgccaaa ggttgtggtc caaccttctg
     3841 tgccgcctgc gggagtgtag atcagggtgc cgttatcctg cgtaccaccg atgatcttgc
     3901 ccgtcgtggc attgcctgcc gcgccgtaga attgggtgat gccgaggttg ttattcagtt
     3961 ccgtccagcc agtggtgccg acgactgtgt acacattatt ggcgcggtac acgccgccat
     4021 cgttaccaaa gtaaacggtt gtgttgcttg tgccattgaa acctggcttt tccacaatga
     4081 catgatggtc ggcatgggcc gattgcggtg aattccacca ttcactgatc tgggtcaggg
     4141 tcgagccgcc gttcgtactt cggtaaaggt ccacgccgcc cacaacaaga gtgttgggat
     4201 tggtcggatc cacccaaagg gcattgtcgt accaaccctg cccggagagg aagtcattgc
     4261 cggagttgac gagtgcatag gaaacgccgc catccacgct cttccaaagc tgcccgccga
     4321 ggttatccac agaagcatac acggttgagg ggctgctcgg ggcataggcc acttctacgc
     4381 ggccattgcc accggtgttg gttgagcccc aggaagcgcc acccgttgtg gaatagtaag
     4441 cctgcccgga gaattgattg cctgcaaccg ccttgtttcc gtcactcggg ttgaagttca
     4501 catcggccat gtacccggta agaaccgaag accaggagga gccgccatcg gtgctacgcc
     4561 acaaaccggt ataggtggcc gccaacagaa tctgcgggtt ggacggatga attgccaacc
     4621 gattcacaaa ataccagttt gaattattgg tggaggacag ttgtgtccat gtgctgccgc
     4681 catccgtggt tttgaacacc ccgccgccga gaggctcgga aatttcacgg ttgcctgtga
     4741 aaccttcacc tgtaccagcg tacaaaatat tcgggttgga cgggtcaatg accatggtcg
     4801 tcacagccat gttggacata aaatcagtca ggatgttcca tgaagcgccg ccatcggtgg
     4861 atttccaaat gccgcccgtc acactgccag cccacatgat attcgtatcg gtggggtgga
     4921 taatgatgga gcgcacacgt ccgccgatgt tgcccgggcc gatccacgtc cagcctgcgc
     4981 tggtgatcgc ctgcggattg tttccctgct gggcgcgcat ttcattgatt tgctgggcag
     5041 ccgtaaacaa gccatccgca gggatattgc cgttctcgtc gcgccaggcc aaagcacgat
     5101 actcgcgggc ctcttcagga tggtccgctt ccttctcatc ggtctccaca tgctgcacgt
     5161 tcgaagacaa tgcctgggtc acctgagtgc tcattgggcc aagcctgccc cacagaccaa
     5221 gcgcaacggc caaaagcatg aggcttgaag cgatggaaag gaacattcga tttcttttcg
     5281 tcatgatcat tctccttgta ataattgggt tcaatgaaaa aactattcct caaaacgcag
     5341 ttcagttact caggtacaag gcacctcctt tttcaaactc actgtccata aataccggca
     5401 aggcggtttt ctggcaaaaa gaaccgctgg acgattccaa acaacaacac agagggcagg
     5461 gtcatcacca ttgaacctgc caaaagcaac ggccagttcg tgcggtccaa ttcctgcaac
     5521 tgacgcagac ccactggcag ggtgtaaaga gtctgcgatt tcaaatagaa caagggcgtg
     5581 gtgaaatcgc cccaatacaa caaaaaggtc agaatgccca ctacgatcat tgtggcattt
     5641 gaaagcggca aagccacccg ccaccaaatt tccagcggac cggcgccatc catccgggcg
     5701 gcatcgtaca gttcacgggg aatgcgccga aaactccaaa agaacaacag cacaaacagc
     5761 ggactcgacc ccatgaccga cggagccagc aaagccagat atgaattaaa caaccccaca
     5821 gtgcggaaca aaataaagcg cgccacccac acagcgacag tgggtacaac caaaaggaca
     5881 acagacaacc cgagaagatt ttcccgccag cggcgattca cctgagacat tgcaaagcca
     5941 gccagcgaag cagtgaccag tgtgatcggc acggcaagac ctgcaacgag cagggaatta
     6001 agaacatacc gccccattgg gagcatctcg aaaatacgcg tgtaattcga ccatgcaaaa
     6061 ggctcgggcc accactccac tgttcgtggc ggaggcaggc ctggttgacg aagcgaagcg
     6121 gaagccatcc aaacgagcgg cagaatgaac agggccgaga tcaagatcga gacggcatga
     6181 aatgcgatcg agtgaaacat cctgcgttgg gaattaaatt gcatcggtct ctccccaact
     6241 acccacaatg gaaagaatga acccgatcaa tgcggcggtg accaagccca acagcacggt
     6301 catggcggag cccgggccaa agtgcattcg gtcaaaggct tcttcgtaga tcagcagcgg
     6361 caaaaacaaa gtggagtaat acgggtcgcc attggtcatg atgaaattgg cggtaaaaat
     6421 attctggctg ctcaaaatga catcgcgcag ggtgagtaaa agcagccacg gtgccagcag
     6481 cggcagggtg atgtagcgga attgctgcca gaaattaccg ccatccacgg cggcggcggc
     6541 ataatattca ggcggaatat tttgcaaccc tgccagcaac accacaaagc cttcgccgat
     6601 ctgaaatgcg gtcatcacca caaaaaccat ttttgcatat tgggcatcgg ccagccaccc
     6661 gggtgtgggc agtcccaacc cttcaaggat caagttcaac gggccataca acgggttgaa
     6721 tatccacatc cagatcaagg cataggccac atcgggaata atggtgggca ggtacaccac
     6781 cgcccgatac aaccccacac ctttgccgcg gcgattgagc aataaggcca atgccagggc
     6841 gctcaacact ctcacaggca cagccaggat cacataatac aaagtgttgt acagcgaaat
     6901 gggaaaaagc aaatcaacag gatttgccag caccaattga aaattaaaac cacccaccca
     6961 cacaggcgcg gaaattccat cgtaacgcag gaacgccagc agtaatgaaa gcaaggcggg
     7021 caagccgacc aacaccaccg cccccaataa gtaaggcgtc agcatcaaaa gcaattgcgt
     7081 gcgggatgaa gcggtataag ttctattcat tttccgtgaa caagggcagg gcacgctcca
     7141 ccgccgattg catggcctcc aaggctgtga cctctccaaa gaatgcgcgt tgtaactcct
     7201 catcgcaaat ttcttcgata tcggcccaat tggcttgttg cggcaaacgc agggtaaagg
     7261 gaatcacatc caaaaacaca cggctgtgtt cgggttttgc attcgggtcg agaaaagcat
     7321 ccgactcagc cacctggatc aacgaaggta cggtgcgccc gctttgcgcg atcagcgatt
     7381 gtccagcggc ggagttggca aattcgatga aggtccatgc atcttccttg tgctggctgg
     7441 ccgcagtcag gcagtaggca tcagaatgaa gaatggtggc atcgcttctt ccaagaggca
     7501 ggggagccac atcccaatca aaagccgtga tctcgcggaa ggtcggcacc aaacggcggc
     7561 tgttgaaata gatggcaaca cgcccgttga taaaacgact ctcactgctc tccgcttttt
     7621 cctcctcggc attcggcaca atgtgatgcg tcacctgcca atctacgaac cattgcacgg
     7681 cttccagatc aagcggctcg gcaagtgtga gcgcattgtt gatgctgacc ttgccgccat
     7741 tctgccaaac aaaaggcgcc acacggatca aggtcggctc cacacctgcg ccgtattgat
     7801 ccacgcggcc atccgaatca acatccaaag tcaacgcttt tgaagcggaa ataaaatcat
     7861 cccaagtcca gcctgcatcg gggtggggaa tccccgctgc atcaaacaaa tccttgttgt
     7921 aatacgccac caggcttgaa atattttgcg gaatgcaaac cacctgatcg ttcagcgaaa
     7981 acgcacgcat cgaaagcgca taaaaatcat catacgcgat cgactctgat ttttcgaggt
     8041 acggtcccag cggctcaaaa gcattcttga gcgccagccc gctgaaacgc cgataattga
     8101 tcaagaccac atcggcgggc tcaccgccta ccagatccgc cgccagccgt ttgcggtaat
     8161 caccctggct gggaatgtgg atcaattcaa ctttaatttc ggggtgctgc ttttcaaaag
     8221 ccgccaccaa atcctggtac gccttcaact cggcaggttc accgaacacc ataaaactga
     8281 cggggcgagg tccctgctgc ccacatgcgg ttgtaaaaag aaagatcacc aataaaaaca
     8341 aaagcgccaa ccgacttcgc atattttctc ctgtttcaac gacgggattt tttagaaaga
     8401 agaagatcaa tttaatcgga tgtcttccct gaaaaagaat cttgaaacca aactgcgcct
     8461 ccaaccgacc tcaaacctga ttctgtccaa tctgcatttc gccaccctaa aaacagactc
     8521 cctacttcac atcgacggtg aaaacgaaaa ccatcggggc agccggtgga caaggcggct
     8581 tggcacaggc aggcgcgggt gcagtcaaac ggatatcagt ttgcccggcg gccactgcac
     8641 gaaacaacca cccttgcgaa ccaggctccg tcattttatc tgcgggtgtc agaggctcaa
     8701 ccacctcggc tgcatatgcc actttccaaa tcgaggaacc tggcggcacg gaaattgcaa
     8761 acacatcccc caccgaaagc ggaatcacct gtccgcgttg gaaggcaggg tcaatcgtca
     8821 ggcttggagc agatggagtt gtggtgggca tttccaagga gggttcctcc gtcatcacaa
     8881 tgactggagt gaaagcggct gttggcgtgg caggggtggc acacgactga ttgaaaaggg
     8941 tcacaatcaa taaacaggcg atgataagat attttttcaa acgaggcatg gatgatattt
     9001 ccaatcacat tggttggaat cattactccc acactataca atttttttta tgattaggca
     9061 ataaaaaccg aggtgatttt cttcctcttt tgtaaggctc gtaatgtggg cttcagtccg
     9121 ctttgaatgg taaatggcag gctaaagtct gcggtacggg tacaaaaaac ccccgcgaag
     9181 attttcgcga gggttttgga ttcaaaactc agaaagctta ttgtttcggc aggctgaagg
     9241 tcttcaccat caagaccgcc atgctggcac gggttacggc atcatcgggg cagaattttc
     9301 cattgccaca accagtggag atgccttcgg cggccaactg cttgatccat gcggcggccc
     9361 aataagaagt tgggacatcg ctaaacccgg tgctgccacc caccgcgggc ggggtgtaag
     9421 cggcaccatg cttggaacgc agaaggaaga cggccatttc agcattggag gctttgcctt
     9481 ccgggcagta caggctggta ccgcatccgc cggtaatgcc ttcggtggcg aattgcttga
     9541 tccatgcggc ggcccaatgt gaagcgggaa catcgttgaa acctgtgctg ctgccaaccg
     9601 caggaggaac ataggccgag ccgtgaatac cgcgcagcag gaagaccgac atttcagcgc
     9661 gggtgacggt gccttcgggg cagtacatca acgggctgga ggaacagccg ccggtgatgc
     9721 cagccgcgta cagacgttcg atgtagttga acgcccaata ggtgctgggc acatcataga
     9781 agatcgagat ggtgtagttc tggccggtct gatgggcagt gacgttcgta taactgcgat
     9841 aggccgggtt gaaatagaaa ttatctttgg agggtgtgac ggtaccggtc cagtttgcgg
     9901 agacggtgaa tttgtaggca ccggtactat cggcaatggc ggtcttggct tgtccgccgt
     9961 cggagtagct taagatggcg cctgccacac ctgcattgcc gctgatctca ggtgtaccgg
    10021 accctgttgg ggtggcggta aagttttgga tcgtttgagc agctgtcagg ttgctgaaag
    10081 aagcgttgat cggggtgaaa taaaatccga ctttggaggg tgtgatcgtg ccggtccatc
    10141 cacttgggac ggtgatcgag tatccgccgc tgccatcgga tgagacggtc ttggtggtgc
    10201 cgctgactac ataggtgagg gtggcgctgg gggtgccaac gttacccaaa atgaccacgc
    10261 ctgcggcgct ctgcggtttg gtggtaaagg tccaccattt ggcgtcgtta gcgtagacga
    10321 atttcttcgg aatacaggtg gcgcaggtaa tggccttgat ctgccagtag tagatcttgt
    10381 cgtattcgag gttggtgagt tttacgctgg tgttgaggta ggtaccggtc cagttcgggt
    10441 cgttatcgtc acaagccgag ccgatcttga cacagtaact gtatttatcg ggggtggggc
    10501 tgtaggtgcc ccatgtgagg gtgaggttct tggggtcgag gttgatggtg ccgctggcgg
    10561 gagaggtttt gctgaaggtg gtggcaaatg gatcgggggc aaaaccagtg aattgggcgg
    10621 cgggagaagg agccgttttt tcactcatcc acacggtgac acaagtgctg gaggagtcat
    10681 cggcacaggt gaggaaatcg acggcgaaat agtagcctgc atcgtggtag aagaccttgt
    10741 gggtgccttc tgcggtgtca taggcatcgt aggaggtcca gccagaagcg gcaagttggg
    10801 cattggaata gtaatcagct acatcctgcg aaacgccgcc ctggaatcgt tctgctgcgg
    10861 catagccctt accagagagg gtgatcgcat cgcctctgac gctggtggta atgctgcgac
    10921 tgacagagcc gagatcggcc caagtgaggc tggaaaatag tggagcttca ggcgcgctct
    10981 ggcgggggga tgcagcaagc acgtcctgtg agttctgcca tgcgccagtg gtcaaaacca
    11041 ttacggccac aacacaggca aaaaacattt tcttgaagac tacggacatt tttatctcct
    11101 tgtttttggt gcttcggctg ggctttcggg tcatcagagc agttatgatg atttgagcaa
    11161 cctgttaaac cagccagcgg gcccgttaaa ttaaaaacag gtacccattt aatcttaagt
    11221 attgtacacc atattaaacg gacgaaaaaa ggaaaatgtt ccgcgcgtag gggaatcgta
    11281 cgtttttcgt ttgcatactt acagggattc aggcgcggat tccattgtct acacgaaaat
    11341 ctatggcgtt ttttgttgtc gttttccagg agcagtggtt caaagttttt ttgttagctg
    11401 cctatgtggg tggcaatccc tgtttcctgt cttgcaaatt tgaaaatgaa acccctccaa
    11461 ttagaggggt gacatcatta cagcctggca ggtaatatta tgaaatggat ctcttgtggc
    11521 atgtgtaggg gaaacaggtg agggcagatg gagttttggg attctccata gtcggtttgc
    11581 tgcaagag
//
LOCUS       DAOQJC010000120        11446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2855_length_11446_cov_8.699500, whole genome shotgun sequence.
ACCESSION   DAOQJC010000120 DAOQJC010000000
VERSION     DAOQJC010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11446
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2855_length_11446_cov_8.699500"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="PLE39_15255"
     CDS             <1..1532
                     /locus_tag="PLE39_15255"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylosucrase"
                     /protein_id="HNE69737.1"
                     /translation="PLFKAPEGENDGGYAVSSYREVHPPLGTMEELASLAHELRNAGI
                     SLVVDLVFNHTSDEHLWAQGAKSGDDEFQDFYRIFPNREMPNLYEQNLREIFPDEHTG
                     AFTFFPNLFKDGGWVWTTFHSYQWDLNYANPAVFNRMAEEMLFLANQGVEVIRLDAVA
                     FIWKQLGTNCENLPAAHVLIQAFNAVARIASPALLFKSEAIVHPDDVVKYISPEECQL
                     SYNPLLMALLWNSLATRKIKLLSQALATRFKINPNTAWVNYVRVHDDIGWTFSDEDAA
                     SFGINGNDHRRFLNEFYRGRFEGSFSRGLPFQENPKTGDCRISGTCASLAGLEKALKE
                     EGPNEVELAIRRIHLIHGVILTAGGIPLIYLGDEIGTLNDYTYLDDPAHERDSRWVHR
                     PKTDWNQYAKRNIQNTTEGRVYQGLKQLIELRKQHTVFSGGELEIIQTENDHVLGFMR
                     TYAGKRSVIFANFSEAPQTLASRIIEQTSVTSLRPLHGRSKLTNYRELKLEPLDFVVF
                     G"
     gene            1575..3098
                     /locus_tag="PLE39_15260"
     CDS             1575..3098
                     /locus_tag="PLE39_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNE69738.1"
                     /translation="MLSRVYSCAVVGLEGVIVEVEVDYTNGLPGVTIVGLPDAAVQES
                     RERVQTAVKNAGLHFPRHRIVVNLAPAVVRKEGPAYDLPIALGVIILAGHLPHDVMDG
                     ALVIGELSLDGVVRHTRGILPMAATARTNGYKRMFVPEVDAPEAALIPGLEIIPVKSL
                     ADLYDHLAGRRLIQPYQPSENILEPLFTPTDFAEIKGQEHVKRALEVAAAGGHNVLMV
                     GSPGAGKTLIARALPGILPEMSIEESLDVTRIYSVADQLPAGTPLIKQRPFRAPHHTI
                     SHAGLVGGGNIPKPGEISLAHRGVLFLDEFPEFGTRVLEVMRQPMEDKVVTISRAKGS
                     LTFSANFQLIAAMNPCPCGFYGDQQKACTCAPAVVTKYQKRISGPLLDRIDIHIEVPR
                     VDYEKLSGNKLGETSETIRKRVQTARDIQNQRFANTTDIVCNADMRIGEVRQFCQLQA
                     EGQSLMRAAMSQLNLSARAYHRILKLSRTIADLAGSEEILSTHLAEALQYRPKIMMG"
     gene            complement(3210..4112)
                     /locus_tag="PLE39_15265"
     CDS             complement(3210..4112)
                     /locus_tag="PLE39_15265"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNE69739.1"
                     /translation="MKKIFKWIGIVLGSLIGLVLLAGVTLYFVGVSRVNKVYDFPPSG
                     IVVPTDAESITRGEHIAEVTCLGCHGRDLGGVSPFFAMEGVASMDTPNLTMGEGGLGS
                     IYSDEDFVRAIRHGINQEGRANSMPPVPYFQYMSDEDLGAVIAYIRSVPPVDKTTKPF
                     QAEPLGYILFAAGMFGEQAADVISHQNNITAPEPGVTVEYGEYILRISACQDCHGVDL
                     AGGPYPDPSVTYIVPNLTPGGDLATWTEEQFLQAIRTQKTPDGRGLNANLMPFDDVNN
                     LTDEELKAIFLYLQSLPKIETQIK"
     gene            complement(4136..4831)
                     /locus_tag="PLE39_15270"
     CDS             complement(4136..4831)
                     /locus_tag="PLE39_15270"
                     /inference="COORDINATES: protein motif:HMM:NF036807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNE69740.1"
                     /translation="MNTQSLKVIGAGLGRTGTESLKQALEILGFGACYHMFELTMKHP
                     EHLPEWEKLIAGEKPNYEILFDGYQSTADFPACMFYREFTAQYPQAKVVLTVRDADKW
                     YDSASKTIFRGVPAPMVAFVRFMGLFSKGARAALPTLIFAKEVVNNHFFEGRTDDRDH
                     TKAIFNAWNEDVQRTVPPDRLLVFDVKDGWEPLCHFLGVPIPSAPFPHANSGEDFKKN
                     NVRRFFNEKTTSK"
     gene            complement(4855..6420)
                     /locus_tag="PLE39_15275"
     CDS             complement(4855..6420)
                     /locus_tag="PLE39_15275"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNE69741.1"
                     /translation="MNKITDLSATQIAQGIKSRKFSAREAVDAHIDHIETVNPKINAV
                     VVPMFDEARKAADEADAAQKRGAALGALHGVPITIKEQFLVKGTPTTMGLPSQKNHRA
                     EEDGPLVKRLRDAGAIILGKTNVSQMLMFIESDNPLYGRTKNPWNLERSCGGSSGGEA
                     AIIAAGGSPLGLGGDFGGSIREPAHFCGIQGLKPTAFRLTNFDTRSDLLPRGQEVIIP
                     QPGPMARKVADLALAMDVLAAPGQELLDPSIPPVPWRNFADVDIKSLRVAVYEDDGYF
                     KAAPAIRRVVREAAEALRGMGAQVVEWTPPAVNELTLLFYSVFKSDDGAALRHALRND
                     EPMPQLKGIIRSKQMPGFMKKLFVKRLKSAGEERFLRIFEAIGMRSAEGYWKLVVERN
                     QHRLRFMRDLEAGGFDAIICPPTSLPAVLHGSTNDLLDFDSYARLFNVLGMPAGVVAA
                     GRVRAGEESDRQPSRDSVEQAALRVETGSVGLPVGVQVAARHWREDVALAVMSALENH
                     FESQSDYPTRPVL"
     gene            complement(6417..6647)
                     /locus_tag="PLE39_15280"
     CDS             complement(6417..6647)
                     /locus_tag="PLE39_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69742.1"
                     /translation="MTNFSQSRPSVYHHFILLVWEECDVMGRHLAWRFSLQDSQKETR
                     IGFKNLEELTVFLERWMKDPSAGDTNQKELTQ"
     gene            6732..>7329
                     /locus_tag="PLE39_15285"
     CDS             6732..>7329
                     /locus_tag="PLE39_15285"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNE69743.1"
                     /translation="MAQLEIRLLGSPQITRDNIPHTDFISNKVPALLAYLAVERRAHS
                     RDKLAALLWGEMTDTDAKNNLRQALANLKKYFEDELTIARDTIEFTGDAFVDSLEFDS
                     ALHSASLLAPEPASVILTDSLRLYRGDFLEGFHVREAPEFEDWMLNERARLRELALQA
                     LHTLTQFHASRGQFNEAKAFASRLLSFDPWREEAHRQLM"
     assembly_gap    7330..7429
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7430..9826
                     /locus_tag="PLE39_15290"
     CDS             <7430..9826
                     /locus_tag="PLE39_15290"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE69744.1"
                     /translation="SRLLSFDPWREEAHRQLMLLQARTGQISAALAQYETCKKILEKE
                     LGVEPSIETTALYERIRSARQTSRHNIPTSSTAFVGRESEMEKLRQRLADPASRLVTL
                     AGLGGAGKTRLAQEIARSCADMFINGAWMVQLAAVDADGLVPAIGNVFDFPFTKGDAK
                     KQLLNFLRQKELLLVMDNFEHLLESSALVSEILEEAHEVKILVTSRERLDLHGEWLVE
                     LPGLAVNSMEANQLFVQSAKRARAEIQVGEEDQAAVSDICRLVGGLPLGIEIAAAWIR
                     SMTVESIRDEIQKSLDFLESSRRDIPERQRSLRAVFESSWARLSEEEQKVFAALSVFR
                     GGFTREAAEAVAGRFDALMDRSLIQRSGERFELHEVTRQFAEEKLSTKKKVRNAHATY
                     FANWASARAKWNERASFAVMRDDFENVRAAWMWAGEQKDVAALSGLVHFTKRYLDIQG
                     RYREGHDLMTRALAQFGASGDVKDLPRDEQGQTIAKLILYRALFLADMGKPDETVPTL
                     EACLEYFRGVGDKPQIMACLNALGIASRFLGLEDKGEGYFRSQLELARELGNRHEEAT
                     ALNNISTALTTLGKNEESESVLRECLALRREMNDEAGISSTLINLAVALFNQGRYEEE
                     KPLLQESIEISRRINQPRNLAGALGNLGTILLKEKNYAGALELFLQGLETHRNTGYRY
                     GIAIALDNVSTAYFHLENDSEALYYIKQSIREAQDIKADFIALDALVWVGGIYARRGE
                     LEKAFEMWEMIRSHNKTDPETIQNLDELMSKFSIGTPLEEQGRMRSFTEIVEEIMQTK
                     "
     gene            complement(9909..>11446)
                     /locus_tag="PLE39_15295"
     CDS             complement(9909..>11446)
                     /locus_tag="PLE39_15295"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE69745.1"
                     /translation="RYTWLPDLKLGLISEKQTSEAKAASDRLATFSLITIVLSVVLIF
                     VVAFTIARGITRPVQLMANTANRLALGDIDQNLDYASRDEYGILADSIRLMLDYQKEM
                     ATVANSISSGNLTESIQPKSEKDQLGQAFKQMIESLREAIQRVAFSANTLTFASEQLA
                     ISADQAGRATSQIATTVQQVAKGITMETESVTRTAFSVEQMSQTIGTVAQGSQDQTKS
                     VTSATHITNQINEAIQQVAGNVQAVTNVSAGAAEAARMGAQTVVGTVKDMQLIKSKVN
                     LSAQRVADMGAQSDQINVILETIEDIASQTNLLALNAAIEAARAGEHGKGFAVVADEV
                     RKLAERAGRATREIGGLIKNIQHTVSEAVIAMDEGGQEVESGVQMANQAGKALEDILK
                     AAEEVYRQAEQADHGAELMRSASSSLVSSVDEVSTVVEGNMAAMQSMTAGMAEMTRTI
                     ENIAAVSEENSAAIEEVSASAEEMSAQVEEVTASAQSLMEMAKSLQEIVQQFNLGESV
                     ASA"
BASE COUNT         2691 a         3186 c         2865 g         2604 t
ORIGIN      
        1 tgccgctctt caaagccccc gaaggcgaaa acgatggcgg ctatgccgtc agcagttatc
       61 gcgaagtgca tccaccgctt ggcaccatgg aagaactcgc ctcgcttgcc catgaactcc
      121 gcaacgcagg catcagcctg gttgttgatc tggtcttcaa tcacacatcc gacgaacatc
      181 tctgggcaca gggagcaaaa tcgggcgatg atgaatttca ggacttttac cgcatcttcc
      241 ccaaccgtga gatgcccaac ctctacgaac agaatctcag ggaaatattt cccgatgaac
      301 acactggagc attcaccttc ttccccaacc tgttcaaaga tggcggctgg gtgtggacca
      361 ccttccattc ctatcaatgg gacctgaact atgccaatcc cgccgtcttc aaccgcatgg
      421 ccgaagagat gttgttcctc gctaatcaag gtgtggaagt gatccgcctc gatgccgttg
      481 ccttcatctg gaagcagctc ggcaccaact gtgaaaacct acccgccgcc catgtgctga
      541 ttcaggcctt caacgcggtt gcccgcattg catcacccgc cctgcttttc aaatccgaag
      601 ccattgtcca ccctgacgac gtggtgaaat acatctcacc cgaagaatgt caactctcct
      661 acaacccgct attaatggcc ctgctgtgga attcacttgc tacgcgcaaa atcaaactgc
      721 tttcgcaagc gcttgccaca cgtttcaaga tcaacccgaa tacagcctgg gtcaattacg
      781 tgcgcgtcca tgatgatatc ggctggacct tttctgatga agatgccgcc tcttttggca
      841 tcaacggcaa cgaccatcgc cgcttcctga acgaattcta cagaggccgt ttcgagggca
      901 gtttttcgcg cggtcttccc tttcaagaga accccaaaac aggcgattgc cgcatcagcg
      961 gcacctgtgc ctcactcgcc gggctggaga aggctttaaa ggaagaaggc ccaaatgaag
     1021 tggagcttgc catccgccgc atccacctca ttcacggggt tatcctgacc gcgggcggaa
     1081 ttccgctcat ctacctcggc gacgagatcg gcaccctcaa cgactacacc tacctcgacg
     1141 accccgccca cgaacgcgat agccgctggg tgcatcgtcc gaaaaccgac tggaaccaat
     1201 acgcaaaacg caacatccaa aacacgaccg aaggacgcgt gtatcagggt ttgaaacaac
     1261 tcatcgaact gcgcaaacag cataccgttt tttcaggcgg cgaactcgag atcatccaga
     1321 ccgaaaacga ccatgttctg ggtttcatgc gcacttatgc gggcaaacgc tccgtcattt
     1381 ttgcgaactt ctccgaagca ccgcaaaccc tcgcctctcg catcatcgaa caaacctctg
     1441 tcaccagcct tcgccctctg cacggacgaa gcaaactcac caactataga gagcttaaac
     1501 tcgaaccact cgatttcgtt gtctttgggt aaacgtattc atcacaaagc ccaaacatca
     1561 tgtaaaataa aaccatgctc tcccgagttt actcctgcgc tgtagtggga cttgaaggcg
     1621 tcatcgttga agtggaagtg gattacacca acggtctgcc gggcgtcacc attgtaggcc
     1681 tgcccgatgc cgccgtgcag gagagtcgcg agcgtgtaca aaccgcagtc aagaatgcgg
     1741 gtttgcattt tccccgtcat cgcattgttg tcaatcttgc accagccgtc gtacgcaagg
     1801 aaggccccgc ttacgatctg cccatcgcgc tcggtgtcat catcctcgcc gggcatctcc
     1861 cccatgatgt catggatggc gcacttgtca tcggcgaact ttcactggat ggcgttgtgc
     1921 gtcacacgcg cggcattttg cccatggccg ccacagcccg caccaatggc tacaagcgga
     1981 tgttcgtgcc tgaggtggat gcgcccgaag cagccttgat ccccggcctc gaaattatcc
     2041 cagtcaaatc acttgcggat ctttacgacc atttagcggg tcggcgcctt attcaaccgt
     2101 atcaaccctc agagaatatt ctcgaaccgc tcttcacccc aacagacttt gctgaaatca
     2161 aaggacagga acacgtcaaa cgcgcgcttg aggttgcagc tgctggcgga cacaatgtgc
     2221 tgatggttgg cagccctggt gctggcaaaa cattgattgc ccgtgccttg cccggcatcc
     2281 tgccagaaat gtccatcgaa gaatcgctgg atgtgacccg catttattca gtggcagatc
     2341 aactccctgc tggcacacca ctcattaagc agcgtccatt ccgcgcaccg catcacacca
     2401 tttcacatgc aggtctcgtt ggcggcggga acattccaaa gcccggtgag atctccctcg
     2461 cccatcgcgg ggttttgttc ctcgatgaat ttcctgaatt cggcacacgt gtcctcgaag
     2521 tcatgcgcca acccatggaa gataaagtcg tcaccatcag ccgcgccaaa ggctcattaa
     2581 ctttttcagc caacttccaa ttgattgcag ccatgaaccc ctgtccctgc ggattctacg
     2641 gagaccagca aaaagcctgc acctgcgcac cagccgtggt gaccaaatac caaaaacgca
     2701 tctccggtcc attgctcgac cgcattgaca ttcacatcga agttccgcgt gtggattacg
     2761 aaaagctcag cggaaacaaa ctcggcgaaa ccagcgaaac catccgcaag cgtgtgcaaa
     2821 ccgccagaga cattcaaaat caacgcttcg ccaacaccac agatatcgtc tgcaatgcag
     2881 acatgcgcat cggggaggta cggcaatttt gtcagttgca ggccgaaggt cagagtttga
     2941 tgcgggcggc gatgagtcaa ctcaatttat cggcacgcgc ttatcaccgc atcctcaaat
     3001 tatcccgtac cattgcagac ctggctggga gcgaagagat tctgtccacg catctggcag
     3061 aggcgctgca atatcgtcca aagatcatga tgggatgaac cacggataat tggtttaaaa
     3121 ataaaatcac tgaagaggtg gttgttttga acatacagga acaagtcttg gataataaaa
     3181 gacttgttcc tgtatgtttc cagacaaagc tattttattt gtgtctctat tttcggcagt
     3241 gattgcaggt acaggaagat cgctttcaac tcttcatcgg tgaggttgtt gacatcatca
     3301 aaaggcatca ggtttgcatt cagcccgcgc ccatcagggg ttttttgtgt acgaattgcc
     3361 tgaaggaatt gctcctccgt ccatgtcgcc aagtccccgc cgggtgtcag gttcggaacg
     3421 atataggtca cactcggatc ggggtatggc ccgcccgcca agtccacgcc atggcagtcc
     3481 tgacaggcgc tgatgcgaag gatgtattct ccatattcaa ccgtcacacc aggctccggt
     3541 gcagtgatgt tgttttggtg cgaaatgaca tccgccgctt gttcgccgaa catccctgcg
     3601 gcaaaaagaa tgtagccgag cggttctgcc tgaaatggct tggtcgtttt atccacaggc
     3661 gggacgcttc gaatatatgc gatgaccgcg ccaaggtctt catcgctcat gtattggaag
     3721 tacggcacag gcggcatgga gtttgccctg ccttcctgat tgattccatg gcggatcgca
     3781 cggacaaaat cttcatcgga atagatcgaa cccagcccac cctcacccat cgtaaggttg
     3841 ggcgtatcca tggatgcaac accttccatg gcaaagaaag gactgacgcc tcccaagtcc
     3901 cttccatgac agccgaggca ggtgacttct gcaatatgtt cgccgcgcgt gatgctttcc
     3961 gcatctgtgg gaacgacgat acccgaaggc ggaaaatcat agaccttgtt gacacgggat
     4021 acgccgacaa aatagagcgt gactcccgcc aacaagacta atccaatcaa cgagccaagc
     4081 acaataccga tccatttgaa tatttttttc attgctaatt ctcctttggc gattctcatt
     4141 tggaagttgt tttttcattg aaaaaccgtc tgacgttgtt tttcttgaaa tcctcaccac
     4201 tattggcatg gggaaaaggc gcggatggaa tcggcacgcc caggaaatgg cagagcggct
     4261 cccacccatc tttcacatca aagaccagca ggcgatctgg cggcacggtg cgctgaacat
     4321 cctcattcca ggcgttgaag atcgctttgg tgtgatctcg gtcatcggta cgaccttcaa
     4381 aaaaatgatt attaaccact tctttggcaa agatgagagt tggcaaggca gcgcgcgcac
     4441 cctttgaaaa caagcccatg aaccgaacga acgctaccat aggggcgggc acgcctctga
     4501 aaatagtttt tgaggcgctg tcgtaccatt tgtcggcgtc ccgcacggtc agcaccacct
     4561 tcgcctgcgg gtattgtgcc gtgaattccc gatagaacat gcaggctggg aagtcggccg
     4621 tggactggta gccgtcgaaa agaatttcgt agttcggctt ttcacctgcg atcagttttt
     4681 cccattctgg caaatgctca gggtgtttca tcgtcagttc gaacatatga tagcaagcgc
     4741 caaagccgag tatctccagg gcttgcttca atgactcggt gccagtgcgt cccagccccg
     4801 cgccgatgac tttcaaggat tgagtgttca ttttgaaatt ctcctttggc gttcttacaa
     4861 aacagggcgc gttggataat ccgattgact ctcgaagtgg ttttccagcg cgctcatcac
     4921 tgccagcgcc acatcttcgc gccaatgccg cgccgcgacc tgcacaccca caggcaagcc
     4981 cacactgcca gtttccaccc gtaacgccgc ctgttcaacg ctgtctcggc tgggctggcg
     5041 gtcgctttcc tctcccgcgc ggactcgtcc cgccgccacc acccctgcgg gcatgcccag
     5101 cacattgaag aggcgggcgt aactgtcaaa gtccagcagg tcgttggtac tgccgtgcag
     5161 gacggctggt aaagatgtcg gggggcaaat gatcgcatcg aagccacccg cttcgaggtc
     5221 ccgcatgaag cgcaggcgat gttgattccg ctccacgacc agtttccaat atccttccgc
     5281 agaccgcatc ccgatcgcct caaagattcg caggaagcgt tcttctcccg ccgacttcaa
     5341 gcgttttaca aacagtttct tcataaaccc aggcatttgc ttgctgcgga taatgccctt
     5401 gagttgcggc attggctcat cgttgcgcaa ggcatggcgc aatgcggcgc catcgtcgga
     5461 cttaaacacg ctgtagaaca agagtgtgag ttcgttcacc gcaggcggag tccattcgac
     5521 gacctgcgcg cccatgccgc gcaatgcctc agccgcttct ctcaccaccc gacggatggc
     5581 aggcgcggct ttgaaataac catcatcttc atacacagcc acgcgcaggg atttgatatc
     5641 cacatcggca aaattgcgcc acggcacagg cggaatggac ggatcgagca actcctgccc
     5701 tggcgccgcc aacacgtcca ttgccaaggc caggtcggca actttgcgcg ccatcggacc
     5761 aggttgtgga atgatcactt cctgcccgcg cggcagaaga tcggagcggg tatcgaagtt
     5821 ggtcagtcgg aacgccgttg gtttcagccc ctgaatgccg cagaaatgcg caggctcgcg
     5881 gatgctcccg ccaaagtcgc cgcccaaccc gagcggtgat ccgcctgccg cgatgatggc
     5941 ggcttcgcca ccgctgctgc caccgcaact gcgttcgaga ttccacgggt ttttggttct
     6001 gccatacagg gggttatccg attcgatgaa catcagcatt tgggacacat tcgtcttgcc
     6061 aaggatgatc gcgcccgcat cgcgcaagcg cttcaccaat ggaccatcct cttcggcgcg
     6121 gtgattcttc tggctgggca aacccattgt ggttggggtt cccttgacca gaaactgttc
     6181 cttgatcgtg atcggcacac catgcaatgc acccaatgct gcgccgcgtt tttgcgcagc
     6241 atcggcttcg tccgccgcct tgcgcgcctc atcgaacatt ggcacgacca ctgcattgat
     6301 tttcgggttg accgtttcaa tgtgatcgat atgcgcgtcc actgcttccc gtgccgaaaa
     6361 ttttcgagac ttgataccct gggcgatctg ggttgcggat aagtctgtga ttttgttcat
     6421 tgtgtcagct ccttttggtt tgtgtcacct gcggacggat ccttcatcca tcgttccaga
     6481 aaaaccgtga gttcttccaa attcttgaat ccgatacgcg tctccttctg tgagtcctgc
     6541 aaactgaatc gccatgccaa atgcctgccc atcacatcac actcctccca taccaaaagg
     6601 atgaagtggt gatacacaga cgggcgcgat tgtgaaaaat tagtcatggg tactgttcat
     6661 tcattctcaa ctgtgataat cttgcgctca ttgtataaag tcatcgttta gaaatcattt
     6721 actggagagc aatggcgcaa ctcgaaatcc gcctgctcgg aagcccgcaa atcacgcggg
     6781 acaatatccc ccacaccgat ttcatctcca acaaggttcc cgccttgctg gcttatcttg
     6841 ctgtcgaacg tcgcgcgcac agccgcgaca aactcgccgc gctcctgtgg ggcgagatga
     6901 ccgacacgga cgcgaagaac aacctgcgcc aagctctcgc caacctgaaa aaatatttcg
     6961 aagatgaact gaccatcgcc cgtgacacga ttgaattcac aggcgacgcc tttgtagact
     7021 cgctggagtt tgactccgcc ctccattccg cttccttact cgcccccgaa cctgcttcgg
     7081 ttatcctgac agattctctg cgcctgtatc ggggtgactt tctcgaaggc tttcacgtcc
     7141 gcgaagcgcc cgagttcgag gactggatgc tgaacgaacg agcccgtctg cgcgaactgg
     7201 cattgcaagc gcttcacacg ctgacccagt tccacgcctc acgcggacag ttcaacgaag
     7261 caaaagcctt cgcatcccgc ctgctttcgt tcgacccctg gcgcgaagaa gcacaccgtc
     7321 aactgatgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cccgcctgct
     7441 ttcgttcgac ccctggcgcg aagaagcaca ccgtcaactg atgctcctgc aagcacgcac
     7501 agggcagatc agcgcggcat tggctcaata tgaaacctgc aaaaaaattc tggaaaaaga
     7561 actcggtgta gaaccttcca tcgaaacgac agcgctttac gaacggatcc gctcggcaag
     7621 gcaaacgtca cgccacaaca tcccgacctc atccacggcg tttgtgggca gggaaagcga
     7681 aatggaaaaa ttacgtcagc gcctggcaga ccctgccagc aggttggtca cattggctgg
     7741 gctgggcggc gcgggcaaga ctcgtctcgc acaggagatc gcgcgttctt gcgccgatat
     7801 gttcatcaac ggcgcgtgga tggtgcaact cgcggcagtg gatgccgatg gcttggttcc
     7861 cgccatcggc aacgtgtttg atttcccatt caccaaaggc gacgcaaaga agcaactgct
     7921 taactttctt cgccaaaaag aattactgct cgtcatggat aatttcgagc acttgctcga
     7981 atcatctgct ttggtaagcg agattctgga agaggcacat gaggtgaaga ttctagtcac
     8041 atcacgcgag cgactcgacc tacacggcga gtggctggtg gagctgcctg ggctggctgt
     8101 caactcgatg gaggcgaacc agcttttcgt ccagagtgcg aagcgtgcgc gggcggagat
     8161 tcaagttgga gaggaagatc aagccgccgt gtccgacatc tgccgtttgg ttggaggtct
     8221 ccccttaggc attgaaattg cagcagcatg gatccgctcg atgacagtgg aatcgattcg
     8281 ggatgagatt caaaagagct tggatttttt ggaatcatca cgcagagaca ttcccgaacg
     8341 acagcgaagt ttgcgagctg tttttgaatc atcgtgggcg agactgagcg aagaagaaca
     8401 aaaagtgttc gcagcattat ctgtctttcg cggagggttc acgcgtgagg cggcagaagc
     8461 ggtggcgggg cgattcgatg cactgatgga tcgatcgctc attcaacgca gcggcgagcg
     8521 attcgagttg cacgaagtga cgcggcaatt tgccgaagaa aagttaagca cgaagaaaaa
     8581 agtgcggaat gcgcatgcca cgtattttgc aaactgggca agcgcgcggg cgaagtggaa
     8641 cgaacgtgcg tcgtttgcag tgatgcgcga tgatttcgaa aatgtgcgcg cagcatggat
     8701 gtgggcgggc gaacaaaagg atgtcgctgc gctttcgggt ctggtgcatt ttacaaaacg
     8761 ctatctcgat attcaggggc gttatcgtga ggggcatgat ttgatgacga gagcgctcgc
     8821 gcaattcggc gcgtcgggcg acgtaaaaga tttgccgcgc gacgagcagg ggcaaacaat
     8881 agcaaagttg attttgtatc gcgcattatt tctggcagac atgggcaagc ccgatgagac
     8941 cgtcccaaca ttggaggcgt gccttgaata ttttcgcggt gttggggata agccgcaaat
     9001 catggcgtgt ttgaatgcgc tgggaatcgc atcacgcttt ttaggattgg aagacaaagg
     9061 cgagggttat ttccgcagtc agttggaatt agcacgtgaa ttgggcaatc gccacgaaga
     9121 agcgacggcg ttgaataata tttcaacagc gttgaccacg ttgggaaaaa atgaagaatc
     9181 agaaagtgtg ctgcgcgaat gtctcgcgct gcggcgcgag atgaatgatg aagcaggaat
     9241 ttcatccaca ctcatcaatc tggcagtggc attgttcaat cagggacgct acgaggaaga
     9301 gaaaccgctc ttgcaagagt ccatcgagat ctcgcgccga atcaaccaac cacgcaattt
     9361 ggcgggcgcg ttgggaaatc tcggtacgat tttattgaaa gagaaaaact acgcaggtgc
     9421 gttggaatta tttttacagg gactcgaaac ccatcgcaac acaggttatc ggtatggaat
     9481 tgccatcgca ctggataacg tcagcacggc gtattttcat ttggagaacg acagcgaggc
     9541 gttgtattac atcaaacaat ccatccgtga ggcgcaagac atcaaggcgg actttatcgc
     9601 gttggatgcg ttggtctggg tgggaggcat ctatgcgcgg cgtggtgaac tggaaaaggc
     9661 gtttgaaatg tgggaaatga tccgcagtca caacaagact gaccctgaga cgattcagaa
     9721 tctggatgag ttgatgtcaa aattctcaat tggaacacct ttggaagagc agggaaggat
     9781 gcgttccttt actgagatcg ttgaagagat tatgcaaacc aaatagggtt acaaaaagca
     9841 ttttccaaat gaaaaaccga agataccctc ttacaacgcc aacatggaca gtgagaggtc
     9901 aggcggattt atgcagaggc aacagactct ccaaggttga attgttgaac gatctcctgc
     9961 agtgatttcg ccatttccat caaagattga gccgaagcag tgacttcctc cacttgggcg
    10021 ctcatctctt ccgccgaagc gctcacctct tcaatggcgg cactgttttc ttcagaaaca
    10081 gcagctatat tttcaatggt gcgtgtcatt tcagccatgc cagcagtcat ggattgcatg
    10141 gcagccatat tcccctccac cacagtgctg acttcatcca ccgatgaaac caaactactc
    10201 gatgcagatc gcatcaattc cgcgccgtga tctgcctgct cagcctgacg atacacttcc
    10261 tctgcagctt tcagaatatc ttccaacgcc ttacccgcct gattcgccat ttgcacccca
    10321 ctctccactt cctgaccgcc ttcatccatg gcgatcacag cttcagaaac cgtgtgctga
    10381 atgttcttga tcaacccgcc gatctcacgg gtggcccttc ctgcacgctc cgccaatttg
    10441 cgaacttcat cggctaccac ggcaaatccc ttgccatgtt cacctgcacg ggccgcttca
    10501 atggcggcat tcaatgccag cagattcgtc tgactggcaa tgtcttcaat ggtttcaagg
    10561 atcacgttga tctggtctga ctgtgcgccc atatcagcca cacgctgtgc cgacaggttg
    10621 acctttgact tgattaactg catatccttc accgtgccca ccaccgtctg cgcacccatg
    10681 cgggctgctt cggctgctcc agctgaaacg ttggttacag cctgcacatt tccagccacc
    10741 tgttgaatgg cttcattgat ctggttcgta atatgggtgg cactcgttac agactttgtc
    10801 tgatcctgtg aaccctgcgc cacggtccca atggtttgtg acatctgctc caccgagaag
    10861 gccgttcgtg tgaccgactc tgtttccatg gtaatgcctt tggccacctg ctgcacagtt
    10921 gtcgcaattt gagaagttgc cctgcccgcc tgatctgccg agatcgccag ttgttcagaa
    10981 gcaaaggtca atgtatttgc gctaaacgca acacgctgaa tggcctcacg cagactttca
    11041 atcatctgct tgaaggcctg tcccaactgg tctttttcag atttcggctg aatactctct
    11101 gttaaattgc cactggaaat ggaattggca accgttgcca tttctttttg ataatcaagc
    11161 atcaaacgaa tagagtcggc aagaatgcca tattcatctc tgctggcata gtcaagattc
    11221 tggtcaatat cgccaagggc gaggcggttg gccgtattgg ccatcaattg cacaggtctt
    11281 gtaatgccgc gggcgatcgt aaacgccaca acaaagatca gaacaaccga aagaacgatc
    11341 gtaatcaaac taaaagtggc aagacgatcc gaggctgcct tggcttcaga ggtctgcttt
    11401 tcagagatca atcccagttt caggtcgggc agccaggtat agcgcc
//
LOCUS       DAOQJC010000121        11436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2859_length_11436_cov_9.446622, whole genome shotgun sequence.
ACCESSION   DAOQJC010000121 DAOQJC010000000
VERSION     DAOQJC010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11436
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2859_length_11436_cov_9.446622"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..1035
                     /locus_tag="PLE39_15300"
     CDS             202..1035
                     /locus_tag="PLE39_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69746.1"
                     /translation="MRRVFIRSFLLLMCILFSACQPVEFGYSPPIIVPVRVSINTAGE
                     IELGLSPELHTPIGTFDISFGADVYSLREEYDARLLIVILDDKATVYKLAEGKEFKIA
                     FDDSNKLYRSVNLKQEDDGDIILQLQSVSVAEEFQGSEEAQVTVEPMSTSTPEPPPCY
                     GVMVTPNDTSKGNILHIEGCPNWVFDSPPFAKGSYAISPNEKFLIYCTTVGDLYAIRF
                     RDQQTINLGNLRKDMPVFHTGDVSIGISISSGENQYFARIVDRISGQSLDFGIPLSIS
                     Q"
     gene            1055..1912
                     /locus_tag="PLE39_15305"
     CDS             1055..1912
                     /locus_tag="PLE39_15305"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE69747.1"
                     /translation="MIPTLPFGRTGHQSTRTIFGAAAFWDTPQKDVDAAMDLVLQNGI
                     NHVDTAASYGKAEEVLGDWIRRHGKPFFLATKTEQRTKQAAYDQIRRSLDLLHVPQVD
                     MIQLHALHEEKDWDTAFGEGGVIEAVIQARAEGLVRFIGVTGHGVPVPEFHLRSLAQF
                     DFDSVLLPYSYIMMQNPRYAENFNKVLAVCKERNIAVQTIKGITRSPWKDVQQNRTTW
                     YRPLEEQADIDLAMHWVFGNPQAFLNTAGDVNLLPRILDAAHRFTTRPSEAQMQDLSQ
                     RLEMAPLFE"
     gene            complement(2012..2452)
                     /locus_tag="PLE39_15310"
     CDS             complement(2012..2452)
                     /locus_tag="PLE39_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69748.1"
                     /translation="MPVSSFIESDLSLLYVMVVGYCTIPELLEHEKMVSRDPARRPNM
                     KIVFDIFNADLDVDLNGIKAGVAYIHTVGRSGWELEPTAILTHNRILETLNQSYELMA
                     HEVVVRNKVFCSLTEALTWLGLSDHRRRVEEIRAQLLREFTLAA"
     gene            2733..4283
                     /locus_tag="PLE39_15315"
     CDS             2733..4283
                     /locus_tag="PLE39_15315"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNE69749.1"
                     /translation="MKKYLLFTVFVSGMTTLAAELAAGRLIGNVFGTSNLVWASIIGL
                     ILIYLTAGYFLGGKWADANPTPAAMYRILAWGAFTIGLVPYVAGPVLRAASTAFDALS
                     VGIMAGSFIAVLILFSVPITLLGAISPFAIRLSVDDTSKAGQTSGQIYAISTLGSFIG
                     TFLPTLVTIPTIGTTKTFLVFGLFLLFVALAGLGKFASKNEMLKLIWMPIVLAVVAIL
                     SAGQALKSNKGQVYETESAYNYIQVQEINGFTLLRLNDGQGVHSIYHPDTLQYNGPWE
                     QFMVGPFFYANRKPQDVQRMAIVGLAAGTAARQATAVYGDIPIDGFELDEKIVEVGQK
                     YFGLNLPNLNIHIGDGRLNLERSPYQYDIIAVDAYRPPYIPPHMTTQEFFEIIASHLT
                     EDGVLTINVGSVPGDRRLIDGLATTIATQFASIHIMDIPGSLNTMIFATKQPTTREDF
                     AANFIALSSDASVEPLLLNTMAVTYTNLTEGYTPTTVFTDDLAPIEWIVNDMVVSFVL
                     QGGLEFLQ"
     gene            4283..4957
                     /locus_tag="PLE39_15320"
     CDS             4283..4957
                     /locus_tag="PLE39_15320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HNE69750.1"
                     /translation="MKPSFLSYLVSLLTPVALIGAALRILLSPIYYNVEYRMPYFPID
                     EYGFTQQDRLQWAPYAVEYLVNSSDISYLGDLQFENGTPLYNERELSHMADVKNVVVG
                     ALRVWYLSLGLLALFAILAQRSGWMPDYLNGLRRGGMWMIGLAVALGLVAGIGITTNP
                     DVFWEFFALFHAIFFEGDSWLFYYSDTLIRLFPIRFWQDAFLWAAILALGGGAGLAFG
                     IRRRDS"
     gene            4976..5839
                     /locus_tag="PLE39_15325"
     CDS             4976..5839
                     /locus_tag="PLE39_15325"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69751.1"
                     /translation="MQPIPSDEVLAKKFGQPRNELIQITDQHTLTVQAWLSSRSPQAA
                     RFNGKGIKASSTGCKIPLLNLALGCDFPKGISAIEIEEEVKAVIDFFAKRNVPWYWWL
                     NTKPSPMDIGQILEKYGLQYDASSLPAMIAPNRQDTTQFPALRKDIRVWRAHSVADLQ
                     AASKIRRLAFRFAEGQAMTYFEDMAADWLDDSSPARLFLAGESESEPMSIGAVIEGAG
                     IPGIYVMATLPDQHRKGYGKAILTRLLAEASASEGRIVALTASQAGAALYSQFGFLHL
                     FDFDFYSLPEE"
     gene            5915..6673
                     /gene="fabG"
                     /locus_tag="PLE39_15330"
     CDS             5915..6673
                     /gene="fabG"
                     /locus_tag="PLE39_15330"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNE69752.1"
                     /translation="MSPTLSLENKVALVTGGSRGIGRAIALEFAARGAAVVVNYNKSP
                     ESAEEVVKQIQSTGGKAAAHQADVSDFKQAEALVKFSVETFGDLSILVNNAGITRDQL
                     IMMMPESDWDAVINTNLKSTFNCSKAAVKHMMRKRVGRIINMASVAGQMGNPGQTNYS
                     ASKGGQIAFTKALAREVAARNITVNAIAPGFVDTEILDAMTPETLEAALKMVPLGRKG
                     KPEEIAFAAAFLASDEAAYITGQVLGVDGGMAMM"
     gene            6763..7110
                     /locus_tag="PLE39_15335"
     CDS             6763..7110
                     /locus_tag="PLE39_15335"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNE69753.1"
                     /translation="MTENTQGKTTVSPDVLTTIARLAALEVPGVSRLAPISGGVNRLF
                     KRGVGDGIRIETEDNTVFVDLHLVLKAEVNIREVSRNVQHSVARAVQEMVGMDVGHVN
                     IHIEDIDYEDNEA"
     gene            7115..7576
                     /gene="nusB"
                     /locus_tag="PLE39_15340"
     CDS             7115..7576
                     /gene="nusB"
                     /locus_tag="PLE39_15340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNE69754.1"
                     /translation="MKPRTRARSIALQVLYETDMAENHLPGDVLQTRLNEAPLAEDLA
                     EFSRQIVFGVLPLTHDLDQLIAKYAPEWPLDQIAAIDRNILRIAFWEFAIQRETPVKV
                     AINEAVELAKLFGSDSAPRFINGVLGTLAENINEIQQVIKNRLAQETKLES"
     gene            7579..7974
                     /locus_tag="PLE39_15345"
     CDS             7579..7974
                     /locus_tag="PLE39_15345"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNE69755.1"
                     /translation="MEILGIGTSELLFIVIIALIILGPKDMQKAGKTIGKWLRNVVTS
                     DGWKIFQQTSRELRTLPNKLIREANEELNQLGNDLNNASDPARNRPNNPIKPPHPQAV
                     PADFPPNKIAPPGVINTAKEKEGDPEQNA"
     gene            7967..8893
                     /gene="tatC"
                     /locus_tag="PLE39_15350"
     CDS             7967..8893
                     /gene="tatC"
                     /locus_tag="PLE39_15350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNE69756.1"
                     /translation="MRNFLSGVWKVITFPFRLVFNILAFPFRKIRQFNQFLNTEPEEH
                     PITEVFANLVTDADTRQMLWDQVEALRMHLLRAILSLVLTVSISFFFTNKVIEFLAGP
                     VGGLGNLKAIEVTETISVFMRVALMCGIALAIPYIAFELWLFAAPGLRSRERKMGLAG
                     IPLAAILFLCGAAFTFFLMLPAALPFLEGFMGVKTELRPESYFSFVIGLMFWIGVSFE
                     FPLIIYVLSAIGIIQPQVLAQQWRLAIVLIAIVAAMVTPTIDPINQGLVMAPMILLYF
                     ISIGLSYIAYAGRKRNNLEDQVNADKAEAGQG"
     gene            8927..>11436
                     /locus_tag="PLE39_15355"
     CDS             8927..>11436
                     /locus_tag="PLE39_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69757.1"
                     /translation="MKKSINPSRRMLVLVVVLALVGQACTLSLLENPFSSGTATQTPG
                     IAVPSATPQAMAQVNFVAVLPEPLQANETLIVTVMDEVTGLAFSDLQYPMTARDSLTY
                     TAVLPLPYNSIVKYRYIRQGNSKILEDSSLGAEIRYRMYYVAGPGEIQDIVSDWSDKS
                     YVRPTGSILGQVFNADTGSPVPNLLVNAGGVQQLTDSTGRFELAGLPVGTQNLVVYSL
                     DGLYQPFQQGAAVADNNATVVNLRVRPTQLVNVTFTVSVPDSTVPGVPVRIAGNILQL
                     GNTFADLQGNVSTNPDRMPILNLQPDGRYSATMALPVGTHIQYKYTLGDGYWNAEHKA
                     DGQWVLREFVVPAQDLAIQDSVETWTAGDAPILFEVTVPSITPPGDIIYIQFNTVGWM
                     EPLPMWPLGNNRWTYKLYGPLNILGSFSYRYCRNAQCGSADDVQTVGPAPAGRQVSTS
                     IMGQDIQDTVNAWKWYENPESVPLVGANITPRASGFIAGVEFQSTYHPNWSFYAPQAL
                     ANTQAIGSNMVVLTPSWTYTRVSPLNFTTVPGKDPLWIDSAIMVSQARALGLNVALFP
                     TPNFASLTTPTSISTNFWRDAPKDSVWWQAWFASYRTFAINYADMAAQTGAQTLILGG
                     EWIAPALPNGILADGTPSNVPADADAQWRSIIAEVRTHFKGQVFWGLPFTKAAIGVPL
                     DFLRDTDGIYLLWSAPLSANPSATKLDYANEAGRLLDNEVATLVSLLNKPLVLAIAYP
                     AASGSANGCVPNGQGGCLDWTELDRPNADIPSASLSLQNQADIYESMLAAVNSRPWIS
                     GFVSRGYYPPASLQDKSASIHNKPAADILWYWYPRLLGT"
BASE COUNT         2691 a         3320 c         2732 g         2693 t
ORIGIN      
        1 ttcaaccccc tccgccaccc cctccgccct agcgggcacc tcccccaaat acgacggtac
       61 ttctgtcgta tttgggggag gacgggtggg ggcgttttag caagctttca agagcattta
      121 cgataaacta tagaaagagg tgggttattc aactgcctcc acctatcagg ctggataatc
      181 aaacccaaca aggaggatgc catgcggagg gtttttatac gaagtttcct gttattgatg
      241 tgtatcctct tcagcgcatg tcaacccgtt gagtttggct actctccgcc catcattgtg
      301 cccgtcaggg tttcgatcaa caccgctggg gaaatcgaac tggggttatc ccccgaatta
      361 cacactccca ttggcacatt cgatatatct tttggcgcag atgtttactc gcttcgtgaa
      421 gaatacgacg ccagactctt gatcgtcatt ttggatgaca aagccaccgt ttacaaactt
      481 gccgagggga aggaattcaa aatcgctttc gacgacagca acaaactata cagaagcgta
      541 aatctcaagc aggaggacga tggcgatatc attctccagt tgcaatcggt atccgtcgcc
      601 gaagaatttc agggaagcga agaagcacag gtcacagtgg aacccatgtc aacctccaca
      661 ccggaaccac cgccttgtta cggcgtcatg gtcaccccaa acgacacttc caaagggaac
      721 atccttcata tcgaaggctg ccccaattgg gtctttgatt ccccaccttt tgcaaaaggc
      781 tcatacgcca tctcgcccaa tgaaaagttc ctgatctatt gcacaacggt cggcgatctg
      841 tatgcaatcc gctttcgcga tcaacaaacc atcaatctcg gcaacctcag gaaagatatg
      901 cccgtatttc atacaggcga tgtatccatt ggcatctcga tcagttcagg tgaaaatcag
      961 tactttgccc gaattgtaga tagaatcagc gggcagagtt tggatttcgg aatcccgctc
     1021 agtatttcac aataaaacca aaaaggagcc acccatgatc cccacccttc cctttggccg
     1081 cacaggccac caaagcacgc ggaccatctt cggcgcggcg gcattttggg acaccccgca
     1141 aaaagatgtg gatgccgcca tggacctcgt cctgcaaaac ggcatcaacc atgtggatac
     1201 agccgccagt tacggcaaag ccgaagaagt gctgggcgat tggattcgac gtcatggcaa
     1261 accgtttttc ctcgccacca aaacggagca gcgcacgaag caggccgcct acgaccagat
     1321 ccgccgctca ctcgacctgt tgcacgtccc ccaggtggat atgatccaac tccatgccct
     1381 gcacgaagaa aaggattggg acacagcgtt cggtgaaggc ggcgttatcg aagccgtgat
     1441 ccaagcgcgt gccgaagggc tggtccgctt cattggtgtg acaggccatg gcgtgcccgt
     1501 acccgaattc catctgcgtt cacttgctca atttgatttt gattccgtat tgctgccata
     1561 cagctacatc atgatgcaga atccgcgcta tgctgagaac ttcaacaaag tgcttgcagt
     1621 ttgcaaagag cggaatatcg ccgtgcaaac catcaaaggc atcacccgtt ccccgtggaa
     1681 agatgtgcag caaaatcgca ccacctggta tcgtccgctc gaagaacagg ccgatatcga
     1741 ccttgccatg cattgggtct tcggcaaccc gcaggccttc ctcaacaccg caggcgatgt
     1801 caacctgctg ccgcgcatcc tcgatgccgc ccatcgcttc accacccgtc ccagcgaagc
     1861 acagatgcaa gacctcagtc aacgccttga aatggcgccc ctgttcgagt aaaacccgca
     1921 tttgaaaaca aacgccgatc tttgctttga tcggcgttat ctatattttt tgggatgatg
     1981 tcattgaaac ttcacccact cacaaccgtt ctcaggctgc taaagtaaac tccctcaaaa
     2041 gctgcgcgcg gatctcttcc acccgccttc tgtgatctga aagcccaagc caggtcagcg
     2101 cctcagtaag cgaacaaaac accttgttcc tcacaaccac ttcatgcgcc atcaactcat
     2161 acgactggtt caaggtttcg agaatgcggt tatgcgtcaa aatggcggtc ggttccaact
     2221 cccagcctga tcggcctact gtgtgaatat aagccacccc agctttgatt ccattcaggt
     2281 ccacatcaag gtccgcgttg aaaatatcga acacgatttt catgttcgga cgtctggcag
     2341 ggtcacggct taccattttt tcatgctcca gcagttctgg aatggtgcaa taccccacca
     2401 ccatcacata tagtaagctc agatctgact cgataaatga agaaacgggc atattctctt
     2461 ttcaaatcct tcgtggcaat aaaagattta tgaaattcaa gttactggct tgaaatcaca
     2521 aactattctg ttttgtacaa gccgcaactt ggagtactta atgaattata accagtactc
     2581 tccgccatag ggtacgccct caaaattaca aaaaagataa atcctcccgt atttttctct
     2641 ttttatcttc cacatcctat ccccctcttt cctcattcat ccctcatcat tcatcattta
     2701 taatccgccc catcctattt cgcggaacat ccatgaaaaa ataccttctc ttcactgttt
     2761 tcgtctcagg catgaccaca ctcgccgccg aattggccgc gggccgttta atcggcaatg
     2821 tgttcggcac gagcaacctc gtttgggcca gcatcatcgg gctgatcctc atttatctca
     2881 ccgcagggta tttcctcggc ggtaaatggg ccgacgcaaa tcccactccc gccgccatgt
     2941 accgcatcct tgcgtggggg gcattcacga tcggcctcgt tccctatgtc gctggacccg
     3001 ttctcagggc ggcttcgacc gcgttcgatg cgctcagcgt cggcatcatg gctgggtcgt
     3061 tcatcgccgt gttgattctg ttcagcgtgc ccatcacatt gcttggcgcg atctcaccat
     3121 ttgccatccg cctctctgtg gatgacacct caaaagcagg tcagacctcg ggccaaattt
     3181 acgccatctc cacacttggc tcatttatcg gcaccttcct gcccacactg gtcaccatcc
     3241 ccaccatcgg caccaccaaa acgtttttgg tcttcggatt gttcttgctt tttgttgcgc
     3301 ttgcggggct cggaaagttc gccagcaaaa atgaaatgct caaactgatc tggatgccca
     3361 tcgtacttgc tgtggttgcc attctttctg cggggcaggc gctcaagagc aacaaggggc
     3421 aggtttatga aaccgaatcc gcttacaact atattcaagt gcaggagatc aacggcttta
     3481 cgttgctgcg cctcaacgat gggcagggcg tacactcgat ctatcatccc gacacattgc
     3541 aatacaacgg cccatgggaa cagttcatgg tcggtccgtt tttttatgca aaccgtaaac
     3601 cacaggatgt ccaacgcatg gccattgtcg ggcttgcagc gggtacagcg gcgcggcagg
     3661 ctacagccgt gtatggcgat attcccatcg acggctttga actcgatgaa aaaatcgtgg
     3721 aggtcgggca aaaatatttc ggattgaacc tgccgaactt gaacatccat atcggtgatg
     3781 ggcgcttgaa ccttgaacga agcccttatc aatatgacat catcgccgtg gatgcctacc
     3841 gccctccgta tattcccccg cacatgacca cgcaggaatt cttcgagatc atcgcctccc
     3901 acctgaccga ggacggggtc ctgaccatca acgtgggctc ggtccccgga gatcgccgcc
     3961 tgatcgacgg ccttgccact acgatcgcca cccagtttgc atccatccac atcatggata
     4021 ttccaggctc gctcaacaca atgatcttcg ccaccaagca gcccaccacc cgtgaagatt
     4081 tcgccgcaaa ctttattgcc ctttcatcag acgcctccgt tgagcccttg ctgctcaaca
     4141 ccatggctgt cacctacacc aaccttaccg aaggctacac ccccaccacc gtattcaccg
     4201 atgacctcgc ccccatcgaa tggattgtga atgacatggt tgtgagtttc gttttgcagg
     4261 gcggcctgga atttttacaa taatgaaacc atcctttctc tcctaccttg tctctctgct
     4321 cacccctgtc gccctcatcg gtgcggcgtt acgcattttg ctttccccca tttattacaa
     4381 cgttgaatat cgcatgccct actttcccat tgacgaatat ggcttcaccc agcaggaccg
     4441 tcttcaatgg gcgccttatg ccgttgagta tcttgtcaac agttccgata tttcctatct
     4501 cggagatctt caattcgaga atggtacgcc gctgtacaac gagcgcgaac tcagccacat
     4561 ggccgacgtg aagaacgttg tcgtcggtgc gctgcgcgtc tggtatctct cgcttggcct
     4621 ccttgcattg tttgccattc tcgcccaacg cagcggatgg atgcccgatt atctcaacgg
     4681 tctgcgccgc ggcggtatgt ggatgatcgg cctggctgtg gcactcggcc ttgttgcagg
     4741 catcggcatc accaccaacc ccgatgtgtt ctgggaattc tttgccctct tccatgccat
     4801 attcttcgaa ggcgattcct ggctcttcta ttattcagat acgctcatcc gcctcttccc
     4861 catccgcttc tggcaggatg ccttcctgtg ggcagcgatc cttgctttgg gcggtggtgc
     4921 agggctggct ttcggcattc gccgcagaga cagttgatct gataaaggcc caaccatgca
     4981 accaattccg tctgacgaag ttctcgcgaa aaagtttggt caaccacgca atgaactgat
     5041 tcaaatcacc gatcagcaca cattgacggt acaggcatgg ctttcctcgc gcagtcctca
     5101 ggcggcaagg ttcaatggaa aaggcatcaa agcatcatct actggatgca aaattccact
     5161 gctcaacctt gcactgggat gtgatttccc caaaggcatc agcgcaattg agatcgaaga
     5221 ggaagtcaaa gctgtgattg atttcttcgc gaagcgaaac gtcccgtggt attggtggct
     5281 gaacacaaag ccatcgccaa tggacatcgg tcagatcctg gaaaaatacg gcctgcaata
     5341 cgacgcctca tcactgcccg ccatgatcgc accaaacaga caggacacca cgcaattccc
     5401 cgcactccgc aaagacattc gcgtctggcg tgcccattcc gtggcagatt tgcaagccgc
     5461 aagcaaaatc cgcaggttgg catttcgttt cgccgaaggc caggccatga cctatttcga
     5521 agatatggcc gctgactggc tcgatgactc cagcccagcc cgactgtttc tggcagggga
     5581 aagcgaatca gagcccatgt ccattggcgc ggtgatcgaa ggggcaggca tccccggtat
     5641 ctacgtcatg gccaccctgc ctgatcaaca tcgcaagggc tatggcaagg ccatccttac
     5701 ccgcctgctt gctgaagcct ccgcctcaga aggccgtatc gtggcattga cggccagtca
     5761 ggcaggcgca gccctgtatt cgcaattcgg ctttcttcat ttattcgact tcgattttta
     5821 ctcactcccc gaagaatgac aagaaatgcc gttcccaaac aggagacagt gggttattgc
     5881 acatgatgta taatttcaat acaggagtca actgatgtca cccaccttat cattggaaaa
     5941 caaagtagcg ctggtcactg gcggttcccg cggaatcggc cgtgccattg ccctcgaatt
     6001 tgccgcacgc ggtgcggccg ttgtggtcaa ttacaacaaa tcacccgaat cagccgaaga
     6061 agtggtcaaa cagatccaat ccacaggcgg aaaagccgcc gctcaccagg cagatgtgtc
     6121 tgatttcaaa caggctgagg cgctggtcaa gtttagcgtg gaaactttcg gcgatctgag
     6181 catccttgtc aacaacgccg gtatcacccg cgaccagttg atcatgatga tgcccgagag
     6241 tgattgggat gcggtcatca acaccaacct gaaaagcaca ttcaattgtt caaaggccgc
     6301 cgtcaaacac atgatgcgca agcgtgtcgg gcgcattatc aacatggcct ccgtagccgg
     6361 tcaaatgggc aaccccggac aaacgaacta ttccgcctcc aaaggtggac agatcgcttt
     6421 caccaaagcc cttgcccgcg aagtagccgc ccgtaacatc accgtcaatg ccattgcgcc
     6481 gggctttgtc gataccgaaa tcctcgatgc catgactcct gaaaccctcg aggctgcgct
     6541 caagatggtg ccgttgggcc gcaaaggcaa acccgaagag atcgccttcg ccgccgcatt
     6601 tcttgcttct gatgaagctg cctacatcac cggccaggtg ctgggtgtgg atggcggcat
     6661 ggccatgatg tagtttggaa attgtgtcga tcattgcaat gacaacactg aaaaagataa
     6721 gaaaacctgt aagcaatcaa ggggttcccc ccaggagtag gaatgactga aaacactcaa
     6781 ggaaaaacca ccgtctcccc tgatgtcctc accaccattg cccgccttgc cgcactggaa
     6841 gtgcccggtg tcagcaggct ggctcccatc tccggaggcg tcaaccgcct cttcaagcgt
     6901 ggagttggtg acggaatccg tattgaaacg gaagacaata ccgtatttgt cgacttgcat
     6961 cttgtcctca aagccgaggt caacatccgt gaggtcagcc gcaacgttca acacagcgtg
     7021 gcgcgtgccg ttcaggaaat ggtcggcatg gatgtcggtc atgtaaacat ccacattgaa
     7081 gatattgact acgaggataa tgaggcctga aagcatgaaa ccccgaacca gagcgcgttc
     7141 catcgctctg caagtacttt acgaaacaga tatggcggaa aatcacctgc ccggtgatgt
     7201 gttacaaacg cgtcttaacg aagcgccgct tgcggaagac cttgcagaat tttcacggca
     7261 gatcgttttt ggcgttctcc ctctcactca cgatctggac cagctgatcg caaaatacgc
     7321 ccccgaatgg ccgctcgatc agatcgcagc catagaccgc aatatcctgc gcattgcctt
     7381 ttgggaattc gccatccagc gagaaacccc ggtcaaggtg gctattaacg aagcagtgga
     7441 actggccaaa ctgtttggtt ccgattccgc gccacgcttt atcaacggcg tgctgggaac
     7501 attggctgaa aacatcaacg agatccagca ggtcatcaag aaccgcctcg cccaagagac
     7561 gaaattggaa tcataaaaat ggaaatcctt ggaatcggca catcggaact gctcttcatt
     7621 gtgatcattg cgctcatcat ccttggtccc aaagacatgc agaaagcggg caaaaccatc
     7681 ggcaagtggc tgcgcaatgt cgtcacatca gacggctgga agatctttca acagacctcg
     7741 cgtgaactgc gcaccctgcc caataaactc atccgtgaag cgaacgagga attgaatcaa
     7801 ctcgggaatg atttgaacaa cgcctctgac cccgctcgaa acaggcccaa caacccgatc
     7861 aagccgccgc acccccaggc tgtccctgca gatttcccgc ccaataaaat tgcgcccccc
     7921 ggcgttataa atacagccaa agaaaaagaa ggcgatcccg aacaaaatgc gtaattttct
     7981 ctccggcgtg tggaaagtca tcacctttcc atttcgcctc gtcttcaaca tccttgcctt
     8041 cccatttcgg aagatccgtc aattcaacca gtttctgaac accgagccag aggaacaccc
     8101 catcaccgag gtgttcgcca atctcgttac cgacgccgac acgcgtcaga tgctctggga
     8161 ccaggtggaa gccctgcgta tgcatttgtt acgggccatt ctttcgcttg tcctcacggt
     8221 aagcatttcc ttctttttta caaacaaagt catcgaattt ctggctggtc cggtcggcgg
     8281 gttgggcaac ctgaaagcca ttgaagtcac cgagacgatc agtgttttta tgcgtgtggc
     8341 cttgatgtgc ggaattgcat tggcaattcc atacattgcc ttcgaacttt ggttgttcgc
     8401 tgctccaggt ttgcgctcac gtgagcgcaa aatgggtttg gcgggcattc cactggcagc
     8461 catcttattc ttatgcgggg cagcatttac attctttctg atgctgcccg ccgccctgcc
     8521 tttccttgaa ggtttcatgg gcgtcaaaac agagttgcgt ccggaatcct acttctcatt
     8581 tgtcatcggt ttgatgtttt ggatcggtgt ctcattcgaa tttccgctga ttatttatgt
     8641 gctgtctgcc attgggatca tccaaccgca agtgctggcc cagcaatggc ggctggctat
     8701 cgtcctcatt gccattgtgg ccgccatggt cacccccacc attgacccga ttaaccaggg
     8761 tctggtcatg gcgccgatga tcctgttgta ctttatcagt atcggcctca gctacatcgc
     8821 ctacgccggg cgaaagcgca acaatcttga agatcaggtc aatgccgata aagcggaggc
     8881 aggccagggt taagcgcgaa gcgttctttt tatccaggag agagaaatga aaaagtctat
     8941 taatccatca cgccgcatgc tcgttcttgt ggtggtgctt gctcttgtcg ggcaggcatg
     9001 tacactttcc ctgctcgaaa atccattttc atcaggcact gcaacacaaa caccagggat
     9061 tgccgtaccc tcggctaccc cacaggccat ggcacaggtc aattttgtcg cagtgttacc
     9121 cgagcctttg caggccaatg aaaccctcat cgtaaccgtg atggacgaag tgaccgggct
     9181 ggctttcagc gatttgcaat acccaatgac cgcccgcgac tcactgacat atacagccgt
     9241 gttgccgctg ccgtataact caattgtcaa ataccgatac atccgtcagg gcaattcgaa
     9301 gatattggaa gatagcagcc tcggtgcaga gattcgctat cgcatgtact atgtggcagg
     9361 cccgggagag attcaagata ttgtttccga ttggagcgac aagtcctatg tgcggcccac
     9421 aggttcgatc cttgggcagg tgttcaatgc agatacgggt tcacctgtac ccaacctgtt
     9481 ggtaaatgca ggcggcgtac aacagttgac cgattccact gggcggtttg aattggcagg
     9541 gctgccagtc ggcactcaaa atctggttgt ctacagcctt gatggtttgt accagccctt
     9601 tcaacaaggc gcggccgtgg cagataataa tgcgaccgtg gtcaatctgc gcgtgcggcc
     9661 aactcaattg gtgaatgtta cctttacggt gtctgtgcct gattcgactg tgcccggtgt
     9721 gccggtgcgt attgcaggca atattctgca attgggaaac acatttgcag acttgcaggg
     9781 taatgtcagc accaatcccg accgcatgcc tatcttgaac ctgcagcctg atggccgtta
     9841 ctctgcaacc atggcattgc cggttggcac acatattcaa tataaataca cgctcggaga
     9901 tggctattgg aacgccgagc acaaagcgga tggtcaatgg gtgttgcgtg agtttgttgt
     9961 ccctgcacaa gaccttgcga ttcaagacag cgttgaaacg tggacggccg gtgatgcgcc
    10021 gatcctgttt gaagtgacgg ttccttcgat tacgccgcct ggagatatca tctacattca
    10081 attcaacaca gttggttgga tggagccgct tccgatgtgg ccgcttggca acaaccgctg
    10141 gacatataaa ctgtacggtc cgctaaatat cctcggctcc ttctcctatc gctactgccg
    10201 caacgcacaa tgcggcagcg cagacgatgt gcagaccgtt ggccctgctc ctgccgggcg
    10261 gcaggtttcc accagcatca tggggcagga tattcaagat acagtcaacg cctggaaatg
    10321 gtatgaaaac cccgagtcgg ttcctttggt tggggcaaat atcaccccgc gcgccagcgg
    10381 atttattgcc ggggtggagt tccaatccac ctaccatccc aactggtcgt tctacgcgcc
    10441 gcaagctctg gcgaatacac aggccatcgg gtccaatatg gttgtcctca ccccaagctg
    10501 gacgtacacc cgcgtttcgc ctttgaactt tacaactgtg cccggcaaag acccgctgtg
    10561 gatcgattcg gcgatcatgg tctcacaagc ccgcgccctc ggcttgaatg tggcgctctt
    10621 ccccacgccg aactttgctt ctctcactac accgacttcg atcagcacaa acttctggag
    10681 agatgctcca aaagattcgg tctggtggca ggcatggttc gccagctatc gtacctttgc
    10741 gatcaattat gccgatatgg ccgcccaaac cggagcgcaa accctcatcc tcggtggaga
    10801 gtggatcgcc cccgccctgc ccaatggaat actggccgat ggcacgcctt ccaatgtccc
    10861 tgcggatgca gatgcgcagt ggaggtccat catcgccgaa gtgcggaccc atttcaaggg
    10921 tcaggtcttt tggggattgc cattcaccaa agccgccatc ggagttcccc tcgattttct
    10981 gcgtgataca gacggcatct acctgctctg gtcggctccg ctctctgcca accccagcgc
    11041 aacaaaactg gattacgcca acgaagctgg gcgcctgttg gataacgaag tcgcaaccct
    11101 ggtttcactt ttgaacaagc cgctcgttct ggccattgcc tacccagccg catcaggctc
    11161 agccaatggc tgtgtgccca acggacaagg cggatgcctc gactggaccg aactggaccg
    11221 ccccaatgcc gatattccgt ctgccagttt aagcctgcaa aatcaagccg acatatatga
    11281 atccatgctg gctgcggtca atagccgccc atggatctcg ggcttcgtca gccgcggtta
    11341 ttatccaccc gcatccctgc aggataaatc tgcatccatt cacaacaaac ccgccgccga
    11401 tatactttgg tattggtatc cgcgcctgct gggaac
//
LOCUS       DAOQJC010000122        11402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2876_length_11402_cov_11.356218, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000122 DAOQJC010000000
VERSION     DAOQJC010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11402
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2876_length_11402_cov_11.356218"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..1447
                     /locus_tag="PLE39_15360"
     CDS             32..1447
                     /locus_tag="PLE39_15360"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNE69758.1"
                     /translation="MTTGLPESKAKRVFNNVLGAMGNTPLVKLERIGRDLPVPLYAKL
                     EFMNPGGSVKDRVGANIIEQAEKRGEIKPGGTIVEATSGNTGVGLAIAAALKGYKTIF
                     VMPDKMSNEKILLLRAYGAKVVITPTAVGPDDPRSYYEVAKKFARETPNAILANQYHN
                     PDNPKTHELSTGPEIWEQTDGKLTDVIIGIGTGGTITGVGRYLKSKNPNITIVGVDIE
                     GSILTEIWQNKGIIPPGAYPKTYKVEGIGEDFLPSTMDINYVDAIERAGDKESFYWAR
                     QLVRQEGIFAGGSSGSAIAGALKYCRKLTPDRLPVVILPDSGSRYLSKFYDDKWMREF
                     GFLSMEFGETALGDLLIAKPNKQLITATLGDSIRKVVSVMHQNAVSQMPVLGADGSLV
                     GLIEEVDLLNHMLDEHDHSNDEKIDALIQNAGAVFPPETPLEDAMPSLTSGYALIVVD
                     HGKPVGILTKIDVLDYVAGKI"
     gene            1506..1973
                     /locus_tag="PLE39_15365"
     CDS             1506..1973
                     /locus_tag="PLE39_15365"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE69759.1"
                     /translation="MPFLLRIWRTLPLWVHFLAAKLVRPRFRAAVAALIFNELGHVLL
                     FKHTYRKFEWGIPAGGLEYREQPADAVRREFFEEAGMHIQVERLLTVVSAREDHHLGV
                     VYLCKVTEGEFRPSLEISEMKYFPVDDLPTMMLTTEKELIKWAVENQATDKLR"
     gene            1996..2379
                     /gene="crcB"
                     /locus_tag="PLE39_15370"
     CDS             1996..2379
                     /gene="crcB"
                     /locus_tag="PLE39_15370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNE69760.1"
                     /translation="MTNLLLVGFGGFIGSILRYLASGYVQQISKGMGFPYGTLTVNVV
                     GCFIIGFLAQLAETHGVFTTESRLFVFVGILGGFTTFSSFGNETFNLARDGQMMGALA
                     NIGANVIVGLLAVAAGRIVSSLIWG"
     gene            2511..3425
                     /gene="prmA"
                     /locus_tag="PLE39_15375"
     CDS             2511..3425
                     /gene="prmA"
                     /locus_tag="PLE39_15375"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNE69761.1"
                     /translation="MNWLEVSLTVDGELAESVADVLARFAPNGVMTEQGVKFNNEEDE
                     GTATGPITVRAYLEVNDQLEETRQKLEESLYYLGMIQPVPTPAYKQIADQNWMEAWKQ
                     HYKPILIGKSLLILPAWLESPEPSRIPIKIDPGMAFGTGTHPTTQLCLELMERSSLVT
                     SPLSQVIDVGCGSGILSIAALKLGAEKVLGVDIDIESVRNSRENADTNGVGDELILGQ
                     GSVTEVLAGQFAFKSAPLVVANILGPILIRLFDAGLADLVEPNGEIILSGILDHQAES
                     VIGAGQAKGLKRGEVRQMGDWVAISMSR"
     gene            3510..4274
                     /locus_tag="PLE39_15380"
     CDS             3510..4274
                     /locus_tag="PLE39_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69762.1"
                     /translation="MKRANNFLLAVTCLALAAMACSAVSGGAEPTPITIPDIPTLPSF
                     DATAIPDVPQPTDVPPSNGGGGDVLFQDDFVPGRGEWGTGSDADRTVEYVNEQLNIKI
                     AAERYFVWTTPNDQDYNKVHMEVTAYNHNADPNVGFGIICNQQFIEDSMYYFAITSNG
                     EYAIVRAALAQDDVFLTNNGQWNTSDLIAKNAESYRIGVDCANGKLALYVDGQLIDSV
                     DDSTYAAGGFALFVWSADQANGADVAFDDFIMTQLP"
     gene            4367..4858
                     /locus_tag="PLE39_15385"
     CDS             4367..4858
                     /locus_tag="PLE39_15385"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNE69763.1"
                     /translation="MNRRKMFLYILLNIFVSASVTGGMLFWYDRNYRAVSQSVVAQVT
                     PVSGEVDSASETDLQTDIPVKISSVVGAGTVTSEIVVVKFEGEGQLDLASWQLKDEDG
                     NTFTFPKLTLYQNGAVQVHTIAGTDTVIDLYWGINDAVWNSGENARLFDAQGNLRAVY
                     RVP"
     gene            4922..6496
                     /gene="dnaX"
                     /locus_tag="PLE39_15390"
     CDS             4922..6496
                     /gene="dnaX"
                     /locus_tag="PLE39_15390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNE69764.1"
                     /translation="MTQALYRKYRPKQWDTVIGQDHVVQTLINSIKADRVGHAYLFAG
                     PRGTGKTTVARLLAKAVNCLNEDPAKRPCNECDHCKAVNENRFMDMIEIDAASNNGVD
                     DVRDLREKINFSPSQGRYKVYVIDEVHMLSGGAFNALLKTLEEPPPHAIFVLATTEIH
                     KIPATVLSRCQRHEFRRVPIDEIVANLKQIASSEHIQVDDEAFALIARQSAGGMRDAQ
                     SLLDQLASTGTKITLALAQQVLGTATSQTVLDVATSILENQPAHGLETIHRALDSGAD
                     PRSLARQIVEYMRGLMLIQMGNIEQVEATRDVKTQMQAHANAFHSNDVLRMMKIFNNA
                     AVDLRGGWQPSLSLELALAEALDAPTESPQITAAPRRTPPKVDSVSKTESQPEVKSAS
                     SPQGEASPVQSESAPEKPILNSNDIVRSWKSLAASLPKSQANLSALLNSVKMIDIQGK
                     TLLLGLASDVLVSKLDKPDQIEAIQKLIKDHFHVDVAVKCIVTNAKGKIPPNVAQDGM
                     VAAAIHHGGEIVDMDD"
     gene            6551..6880
                     /locus_tag="PLE39_15395"
     CDS             6551..6880
                     /locus_tag="PLE39_15395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNE69765.1"
                     /translation="MAKGFNRGPAMGGQSGMLQQLQQMQKKMEIAQAQLAEETVSATA
                     GGGAIKVVMTGDQKCKSVEISPEFLKDADAEMLQDMVLSAVNMALDESRALQEKLMGP
                     LAGGLPF"
     gene            6951..7556
                     /gene="recR"
                     /locus_tag="PLE39_15400"
     CDS             6951..7556
                     /gene="recR"
                     /locus_tag="PLE39_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNE69766.1"
                     /translation="MILPESIQSLVTALERLPGIGPKSASRLAFYLLRASEDVAQDLS
                     VALANLKANTAFCQECFNITDAGRERCEVCESTKRDGSLICVVEEALDVLALERTGGF
                     HGKYHVLQGVLSPIEGIGPDDLKIKQLVARVANGGVKEIILATNPSMEGDATAQYLQS
                     QLRAYNVHVTRLARGLPVGGDLEYADQNTLLRALSGRQEMN"
     assembly_gap    7557..7656
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7657..8819)
                     /locus_tag="PLE39_15405"
     CDS             complement(<7657..8819)
                     /locus_tag="PLE39_15405"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNE69767.1"
                     /translation="MEENLAGKTCFVIMPFGEKKDLDGQPINFDLIYRDFIKDAVEGL
                     DLKCIRCDEIAEAGWIHSKMFEYIFSADVAVVDITSLNPNVFYELGVRHALKKSVTIL
                     IRKSGTPTPFNIQGFQMLEYKPDDLQNLENGKQKIRDFIKNGIAGQVLDSPIYEVLDN
                     LKVERKPKRIGAKNIHLYPITKVAGKEIGMITGDIQNIKEIDVWVNSENTNMQMARHF
                     ERSISATIRYLGAKRDRAGRVVDDLIANELREAAGTADVPAGIVIPTGSGELNKSHNV
                     KKIFHAASVIGQVGRGYMPITEIAECVRNALELIDSEEMAAENIQSILFPLMGTGTTR
                     LDADEISRQLIDAAISYVEENPQTKVQKIFFLAYHEEDLNICRHILEHDARIT"
     gene            complement(8833..9423)
                     /locus_tag="PLE39_15410"
     CDS             complement(8833..9423)
                     /locus_tag="PLE39_15410"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNE69768.1"
                     /translation="MKKVVITQKALALDDFFQVEEAHLQYELFNGEMSRPVRRFSLER
                     GNSAAVLVYNLDTQKIILINQFRYPTYKNGHGWLTEIIAGMVDPGEKPEESARRELLE
                     EAGLVVSAFETICSFYPSPGGSSEMVFLYYAEVSGEFALYNKTGGLPGHGEDIISLEM
                     TLDNALEDIREGKIVDAKTIMAIVWLENRMLKKSHS"
     gene            9598..10845
                     /locus_tag="PLE39_15415"
     CDS             9598..10845
                     /locus_tag="PLE39_15415"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE69769.1"
                     /translation="MSLPVYRRKFQTFISSVNASNEVAEQIEHWLTDKAGIPVLHDIQ
                     NFQSGASMEDGLELCQSMLVLITKRGIESGRVKKEFELGVKQQARFGNLFHVIPVRVE
                     ECIIPDYLEHSAFVDTVESGFDMSAAMKLLSGLYSNGLVSGDGKTHDLFVSCPWSREE
                     DALTKLVFKLAKRIGFRLIGNPIETHEKSDVSTSLISGCGGGIAVIPDGIGEKTMNKI
                     ISDLGIMSDLNLPFLVVMEKVVNLPDLITQHALEIVHLDKDTLSQDADLEKQIRASVI
                     RLYEDWVSPLEQHYIFYGTDLKEEHKARSRFVRRAIQQITSMPCMMGEDIQRGQIQNE
                     IINMIIGARMMIADISRENLNTCIEAGVAIGSHVPLNLLSGDERHKPPFMFRDRQIWH
                     YHNELDLLGMVHKLVAPYRKFVI"
BASE COUNT         2856 a         2691 c         2840 g         2915 t
ORIGIN      
        1 cggtaaaata cccacaacca aaggagagag aatgacaaca ggattacccg aatcaaaagc
       61 gaaacgtgtc tttaataacg tacttggcgc aatgggcaat acgccgcttg tcaaactgga
      121 gcggatcggg cgcgacctgc ctgttccact gtatgcgaaa ttggaattta tgaacccagg
      181 cggttcggtg aaagacaggg tgggggcaaa catcattgaa caagccgaga aacgtggcga
      241 gatcaagccg ggcggaacca ttgtcgaagc cacctcgggt aatactggcg ttggccttgc
      301 cattgccgct gcgctcaaag gctacaaaac catcttcgtc atgcctgaca agatgagcaa
      361 tgaaaagatt ttattgttgc gggcgtatgg cgcaaaggtg gtcatcaccc caactgcggt
      421 cggtcccgat gatccgcgct cgtattatga agtggcaaag aagttcgcgc gagaaacccc
      481 caatgccatt ctggccaatc aatatcacaa ccccgataac ccgaaaaccc acgagctgag
      541 caccggccct gagatctggg agcagaccga tggcaagctg accgatgtca tcatcggcat
      601 tggcacaggc ggaaccatta ccggtgtggg gcgttacctc aaatctaaga atccgaacat
      661 tacgatcgtt ggtgtggata tcgaaggctc gatcctcact gagatctggc agaataaagg
      721 catcattcct ccgggcgcgt atcccaaaac ctacaaagtg gaaggaatcg gcgaagactt
      781 tttaccctct acaatggata tcaactatgt ggatgccatc gaacgcgcag gagataaaga
      841 gtcgttttat tgggcgcgtc aactggtgcg gcaggagggc atttttgcgg gcggttcctc
      901 tggctccgcg attgcaggcg cgctcaagta ttgccgcaag ttgacccccg atcggctgcc
      961 tgtggtcatt ctccctgatt ctggttcgcg ttatctttca aaattttacg atgacaagtg
     1021 gatgcgcgaa ttcggtttcc tttcaatgga atttggagaa acggctcttg gcgacctgtt
     1081 gatcgccaag cccaacaaac agttgatcac cgcaacattg ggcgactcga tccgcaaggt
     1141 ggtttcagta atgcaccaga atgcagtatc gcaaatgcca gttctcggcg ccgatggatc
     1201 gctggtcggc ctcatcgaag aagtggatct gctcaatcac atgctcgacg aacacgacca
     1261 tagcaacgac gaaaagatcg atgcgctcat acaaaatgcg ggcgcggtct tcccgcccga
     1321 aactccgctt gaagatgcca tgccctcgct cacatcaggc tatgcattaa tcgtggtgga
     1381 ccacggcaag ccagtcggca tcctcacgaa gatcgatgtg ttggattacg tggcaggcaa
     1441 gatttaggac gatttgaacg cccagttgaa agataaaact ttcaattggg cgattgaaac
     1501 ttatgatgcc tttcctgtta cgaatctggc gcactttgcc tttgtgggtg cattttctgg
     1561 ctgcgaaact ggtgcgtccc agattccgag cggcggtggc ggcgctgatc ttcaatgagt
     1621 tgggacatgt actgctgttt aaacacacct atcgcaaatt tgagtggggc atccctgcag
     1681 gtggtttgga gtatcgtgaa caacccgctg atgcggtcag gcgtgagttt tttgaagaag
     1741 cagggatgca tatccaggtg gaacgcttat tgaccgtggt cagtgcgcgc gaagatcacc
     1801 atttgggtgt ggtgtacctg tgcaaggtca cagaagggga attcagaccg tcattggaaa
     1861 tttcagagat gaaatatttt cctgttgatg atttacccac gatgatgctc accacagaaa
     1921 aggaattgat aaaatgggcg gtggaaaatc aagccacgga taaactcaga tgaacatagg
     1981 aacaaaggaa atcagttgac caatcttctc ctcgtcggtt ttggcggttt tatcggctcg
     2041 atcctgcgtt atcttgcaag cggatacgtt caacagattt cgaagggcat gggctttccc
     2101 tatggcacac tgaccgtcaa tgtggtcggc tgttttatca tcggctttct ggcccaactt
     2161 gccgagacac acggcgtatt tacgactgag tcacggcttt ttgtttttgt tggcattctt
     2221 ggcggattca ccacattttc ctcgtttggt aacgaaacgt tcaatcttgc ccgtgatgga
     2281 cagatgatgg gtgcgctggc aaacatcgga gcaaatgtga ttgtgggact tctggctgtg
     2341 gcggctggca gaatcgtttc atccttgatc tggggttgat ggaacaactc aaaatgcggc
     2401 atgtgttccg tagccaaaga acggctgctt tggcggccgt ttttttatct ctgacagttt
     2461 tggtcgtttg gttgtaaaat tcggcaccta ttattttccc tggagtaaat gtgaactggc
     2521 ttgaagtatc cctcactgta gatggcgaac tcgccgaatc tgttgccgat gtgttggcgc
     2581 gctttgcacc caacggtgtg atgaccgaac agggtgtcaa attcaacaac gaagaggacg
     2641 aaggcacagc cacaggcccc atcacggtcc gtgcttattt ggaagtgaac gatcagcttg
     2701 aagagacgcg ccaaaaactg gaagagtcgc tttactattt ggggatgatc cagcccgtgc
     2761 cgactcctgc ctacaagcag atcgcggatc aaaactggat ggaagcctgg aaacagcact
     2821 ataagccgat tctcatcgga aagagtttgc tgatcctgcc tgcctggttg gaatccccgg
     2881 agccgagccg tattcccatc aagatcgatc ctggcatggc atttggaaca ggtacgcacc
     2941 ccacgacaca gttgtgcctg gagttgatgg agcgctcatc actcgttaca agtcccttgt
     3001 cgcaggtcat tgatgttggc tgcggttctg ggatactttc gattgccgca ttgaaacttg
     3061 gcgcggaaaa ggtcttgggt gtggatattg acattgaatc ggttcgcaac tctcgtgaga
     3121 atgccgatac aaatggagtg ggggatgaat taatcctcgg gcagggttca gtgacggaag
     3181 tgctggcggg gcagttcgct ttcaaatcgg cgccgttggt ggtggcgaat atcctgggcc
     3241 cgattttgat tcgccttttt gatgcagggc tggctgatct ggtggagcca aacggcgaga
     3301 tcattttaag tggcattctc gaccatcagg ctgaatctgt cattggggct gggcaggcca
     3361 aagggttgaa aagaggcgag gtgcgccaaa tgggtgattg ggttgcgatc tccatgtctc
     3421 gttaaatcca cacattcccc ataatgtact atttagttat tggcacagac tgataaaatc
     3481 ccgccatcat aattttagga gaatgtatca tgaaacgtgc caataatttt cttcttgctg
     3541 taacctgtct tgcgcttgcg gccatggcct gtagcgcggt ttcaggtggc gcagaaccca
     3601 ccccgatcac catccccgac atcccaaccc tcccgtcttt tgatgccaca gccattcccg
     3661 atgtgccgca gccgaccgat gtgccgcctt caaatggtgg tggcggtgat gttttgttcc
     3721 aggacgattt cgtccctggc cgtggcgagt ggggtactgg ctccgatgcc gaccgcaccg
     3781 tggaatatgt gaatgagcag ttgaacatca agatcgctgc tgaacgctat ttcgtgtgga
     3841 ccacacccaa cgatcaggat tacaacaagg ttcacatgga agtgactgcc tacaaccaca
     3901 atgccgatcc gaatgtgggc tttggcatca tctgcaacca gcaattcatc gaagattcaa
     3961 tgtactactt tgcgattacc tcgaatggcg aatatgccat tgtccgtgcg gctttggcgc
     4021 aggatgatgt cttcctgacc aacaacggtc agtggaacac ctcagacctg atcgccaaga
     4081 atgcagagtc ctatcgcatc ggcgtggatt gtgccaacgg taaattggcc ctgtatgtgg
     4141 acggccagtt gatcgactcg gtggacgatt ccacctacgc tgcgggcggc tttgctctgt
     4201 tcgtgtggag cgccgatcag gccaacggtg cggatgtggc ctttgatgat ttcatcatga
     4261 cccaactgcc ctaaaacggt tttgattttc aaagtcctgt ggtcggtaga tcacaggact
     4321 tttttatttt ccgcctgcgc acattctgtg ctatgattca agcaccatga accgccgtaa
     4381 aatgtttttg tacatcctcc tcaacatctt cgtctcagca agtgtgacgg gggggatgct
     4441 gttctggtat gaccgcaact accgcgctgt cagtcagtct gttgtggcgc aggtgacgcc
     4501 cgtcagcggc gaggtggatt ctgcctcaga aacagacctg caaacggata ttcctgtgaa
     4561 aatcagcagt gttgtgggcg caggcacggt cacctctgag atcgttgtcg tcaaatttga
     4621 aggcgagggc cagttggacc tggcctcctg gcaattgaaa gacgaagacg gaaacacctt
     4681 caccttcccc aaactcaccc tttatcaaaa tggagccgtg caggttcaca ccattgctgg
     4741 tacagatacc gtcattgacc tgtactgggg catcaacgac gccgtatgga attctgggga
     4801 aaatgcccgc ctattcgatg cacagggcaa tttgagggct gtgtaccgag ttccgtaata
     4861 ttcacaaaaa cgttcaacct gaaaccttca acctgacact tgacacctga aacctaaaca
     4921 catgacccaa gctctgtaca gaaaataccg cccaaaacaa tgggatacag tcatcggaca
     4981 ggaccacgtt gtccagacac tcatcaattc catcaaggct gaccgtgtag gtcatgccta
     5041 ccttttcgca ggtccacgcg gtacagggaa gactactgtg gcgcgtttgc tggcaaaggc
     5101 ggtcaactgt ctcaacgaag accctgcaaa aaggccctgc aacgaatgcg accattgcaa
     5161 agctgtcaac gaaaaccgct ttatggacat gatcgaaatt gacgccgcct cgaacaatgg
     5221 cgtggatgat gtccgtgatc tgcgcgagaa gatcaatttt tcaccttcgc agggcagata
     5281 caaagtttat gtgatcgatg aagtacacat gctttctggc ggcgcgttca atgccttgct
     5341 taaaacgctt gaagaaccgc ccccgcacgc cattttcgtt ttggcgacca ctgaaattca
     5401 caagatccct gccacggttc tatcgcgatg tcagcgccat gaatttcggc gtgtgcccat
     5461 tgatgagatc gtcgcaaacc tgaagcagat cgcctcttct gaacatattc aagtggatga
     5521 tgaagccttt gccttgatcg cccgtcaatc agcgggtggg atgcgcgatg cgcaatccct
     5581 gctcgaccag ctcgcatcta cgggcacaaa gatcacgctg gctcttgcgc agcaggtcct
     5641 tggcacggcc acgagtcaaa ccgtccttga tgttgccaca tccattcttg aaaaccagcc
     5701 tgcccatggg cttgaaacca ttcaccgcgc cctcgattct ggagcagatc cgcgctcgct
     5761 ggcccgccaa attgtcgaat acatgcgtgg cctcatgctc attcaaatgg gcaacatcga
     5821 gcaggtcgaa gccacacgcg atgtcaaaac tcaaatgcag gcccatgcaa acgcatttca
     5881 ctccaacgat gtgcttcgca tgatgaagat cttcaacaac gcggcagtgg atttgcgcgg
     5941 cggctggcaa ccctctttga gcctggaact tgccctcgca gaagcactgg acgccccgac
     6001 agaatctcct caaatcaccg ccgcgccgcg tcgcaccccg ccaaaggttg attctgtttc
     6061 taaaacagaa tcccagcccg aggttaagag cgcatcgtcc ccgcaggggg aagcgtcgcc
     6121 cgtccaaagt gaatctgctc ctgagaaacc gattcttaat tccaacgaca ttgtcagatc
     6181 gtggaagtcg ttggcggcgt cgcttccaaa atcgcaggcg aatttatcgg cattgttgaa
     6241 ttcggtgaag atgatcgata tccaaggcaa aacgctcctg ctcggcctcg ccagtgatgt
     6301 gcttgtttcc aaactcgata agcccgacca gatcgaagcg atccaaaaat tgatcaaaga
     6361 ccacttccac gtggatgttg cggtgaaatg cattgtcacc aacgccaaag gtaaaattcc
     6421 tccgaacgtg gcgcaggatg gcatggtggc tgccgccatt caccacggcg gcgagatcgt
     6481 ggatatggat gattaggaat atctgatgct tgcatggtga gcatcgaata aaaacttacg
     6541 aggtttaatt atggctaaag gattcaatcg tgggcccgca atgggcggac aatcaggcat
     6601 gttgcagcag ttacagcaga tgcaaaagaa aatggaaatt gcacaggcgc agcttgctga
     6661 agaaacggtc agcgcaacgg ctggcggtgg tgcaattaaa gttgtgatga cgggcgacca
     6721 gaaatgcaaa tccgttgaaa tttcccctga atttctcaaa gacgcagatg ccgaaatgct
     6781 tcaagacatg gttctctccg cggtcaacat ggcgttggac gaatcgcgcg ccttgcaaga
     6841 gaagttgatg ggtccgcttg cgggtggttt gcctttttag ggtgcaagtt acaggttgaa
     6901 agttgcagga ttgtttttca atctgtaacc ttctaccttc aaccttcaac atgatccttc
     6961 ccgaatcgat ccaaagttta gtcaccgccc tcgaacgcct gccaggcatt gggccaaagt
     7021 ctgcttcacg gctggctttc tatttgttgc gtgcatccga agatgtggcg caggatttgt
     7081 ctgtggcgtt ggcaaacctc aaagcgaata ccgctttttg ccaggaatgt ttcaacatta
     7141 ccgatgcagg ccgtgagcgc tgtgaagtat gcgagtcgac caagcgtgat ggctcgctca
     7201 tctgtgtagt ggaagaagcg ttggatgtgc ttgccctcga acgcacaggc ggttttcatg
     7261 gaaaatatca tgtcttgcag ggagttttat ctcccatcga aggcattggc cccgatgatt
     7321 tgaagatcaa gcaattggtg gcccgtgtgg cgaatggggg agtgaaagag attatccttg
     7381 ccaccaatcc cagcatggaa ggcgatgcaa cggcgcaata tctgcaaagt caattgcggg
     7441 cgtacaatgt gcatgtcaca cggctggcac gcggtttgcc cgttggcggc gatctcgaat
     7501 atgccgatca aaataccctg ttgagagcat tatctggaag acaggaaatg aattaannnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntggg tgatgcgtgc atcatgttcc
     7681 aggatgtgcc tgcaaatatt caaatcttcc tcgtgatagg ccagaaagaa tattttttgg
     7741 accttcgtct gcggattttc ttccacatat gaaatggccg catcgatcaa ttgcctggag
     7801 atttcgtctg catcgagccg cgttgtacct gttcccatca acggaaagag gatggattgg
     7861 atgttttcag cagccatttc ctccgagtcg atgagttcca gtgcgttgcg aacacattca
     7921 gcgatttctg taatgggcat gtatccacgc ccaacctgcc cgatgactga ggcggcatgg
     7981 aatatttttt tgacattgtg tgatttgttc aattcacctg aaccagtggg aatgacgatc
     8041 cctgctggca catcggcagt ccctgctgct tcgcgcagtt cattcgcgat caaatcgtca
     8101 accacgcgcc cggccctgtc tctttttgcg ccaaggtagc gaatggtggc cgatatggag
     8161 cgttcaaaat gccttgccat ctgcatgttg gtgttttccg agttcaccca gacgtcaatt
     8221 tccttgatgt tttgaatgtc acctgtgatc attccgatct cttttccagc gaccttggta
     8281 ataggataga ggtgaatatt cttggcgccg attctctttg gtttgcgttc tacttttaga
     8341 ttatcgagaa cttcataaat tgggctgtcc aaaacctgtc ccgcaatccc attcttgatg
     8401 aaatctcgaa tcttttgttt tccattttcc aggttttgca ggtcatctgg tttgtattcc
     8461 agcatttgaa acccctgaat gttaaatggg gtgggggtgc cagatttacg gatcaaaatg
     8521 gtcacgcttt ttttcaacgc atggcgaact cccagctcgt aaaacacatt agggttgagg
     8581 gaggtaatat ctacaaccgc cacatccgca ctgaaaatat attcgaacat cttggaatgg
     8641 atccagcctg cttcagcgat ttcgtcacag cggatgcact tcaggtcaag accctccacg
     8701 gcgtccttga taaagtccct gtaaataagg tcaaaattga tgggttgtcc atcaaggtct
     8761 ttcttttccc caaacggcat gataacaaag caggtctttc cagctaaatt ttcttccatg
     8821 agatttctcc gtttatgagt gggatttctt caacatgcga ttttccaacc atactatggc
     8881 catgatggtc ttggcatcca caatttttcc ttcgcgaatg tcttccagag cattatccag
     8941 ggtcatttca agcgagatga tatcttcgcc atgaccgggt aatccgccag ttttattata
     9001 taaagcgaat tccccagata cttctgcgta ataaagaaaa accatttctg aactgcctcc
     9061 tggggatgga taaaaactgc aaatggtctc aaatgcagag accaccagac cagcctcttc
     9121 caacaattcc ctgcgtgccg attcttcggg cttttctcca gggtcaacca ttcctgctat
     9181 gatctcggtc agccagccat gtccattttt gtaggttgga tatcgaaact gattgatcaa
     9241 aatgatcttt tgggtatcga gattgtaaac caggacagcg gcggaattgc ctcgctcaag
     9301 gctgaaacgg cgtactggac gactcatctc cccgttgaat agttcgtact gtaaatgcgc
     9361 ttcctctacc tggaaaaaat catcaagggc tagggccttt tgggtgatta ccactttttt
     9421 cataacacct tccttgtttc ataccgctaa ctgttttgaa gggtatatca gaacgatttt
     9481 gaattatcca gcagggattt cacgaataac ttcaagtata ctttaatcca ctgccattga
     9541 ttaatgcgct tgaacttttt tgtaagttga ccgatcttat tatccaagga caggcaaatg
     9601 agtcttccag tatacaggcg gaagttccag acatttatca gctccgttaa tgccagtaat
     9661 gaagtggcgg aacaaattga gcattggctc acagataaag ctgggattcc tgttttgcat
     9721 gatatccaaa actttcagtc cggtgcttca atggaagacg gcctggagtt atgccaatcc
     9781 atgctcgttt tgatcaccaa acgcgggatc gagtctggac gagtaaaaaa agaatttgaa
     9841 ttgggggtca agcaacaggc caggtttgga aatttattcc atgtcattcc cgtccgtgtt
     9901 gaagaatgca ttattcctga ttacctggag cattctgctt ttgttgacac cgttgaaagc
     9961 ggatttgaca tgtctgctgc gatgaaattg ttgtctggcc tttattccaa tggattggtt
    10021 tcaggagatg gaaaaactca tgaccttttt gtcagttgtc cctggtctcg tgaagaagat
    10081 gccttaacaa agcttgtctt caaacttgcc aagcggatcg gcttcaggtt gattgggaac
    10141 ccgattgaaa cacacgagaa aagtgatgtg tccaccagtc tcatctcagg atgtggggga
    10201 ggtattgccg ttatcccaga cggtattggt gagaaaacca tgaataagat catctccgat
    10261 ttggggatta tgagtgattt gaacttgcct tttctagtgg tgatggaaaa ggtagtaaat
    10321 ttgccagatt taatcactca acatgccctt gaaattgtac atcttgataa ggatacatta
    10381 tcccaagatg cagatctgga aaaacaaatc cgtgcatccg tcattcgctt gtatgaagat
    10441 tgggtatcac cattagagca gcactatatt ttttatggga ctgatctgaa ggaagaacat
    10501 aaggcgagaa gccgttttgt gcgtcgcgcc atccagcaaa tcacatccat gccttgtatg
    10561 atgggagagg atatccagcg tggacagata cagaacgaaa ttattaacat gatcatcggc
    10621 gcgcgaatga tgatcgcgga tatctcaaga gagaatttaa atacatgcat agaagccgga
    10681 gttgccatcg gctcgcatgt cccattaaat ttgttgtcag gtgatgagcg tcataaacca
    10741 ccgtttatgt ttcgagatcg ccaaatctgg cattatcata atgaattgga tttactcgga
    10801 atggtccata aacttgttgc tccttatcga aaattcgtca tatagcaaaa ctaatgaaat
    10861 ttgtaagcat gaatagttta tcggtcagtg tgcaggaagt gaagatggcg ctcgcaatga
    10921 gattgaagta atagagttgc catttcttgt ttttaaaaag tatagattct gtgttctaaa
    10981 aaatgattta aattggtgcg attttttaag gtttaaatgc cttgtagata ttgacaaagt
    11041 tttagagatg catataattg ccattcgcca gtacaaaaca aaagatttcg tctccccgac
    11101 aaatcaatga caactgagta accgtctagc atcagagaca caaagcaaat tgttttgcag
    11161 cctgatgagc cgatgcgaaa gaaaaagcat cggtatttta gtctgtgtaa acccttgaca
    11221 gacggttata gtagcgagta gaataccgct cgctcaaatg gcaagcacct taacaactga
    11281 aaagtgatag gaaacaacat ttttgttgaa ccagtaaatt ccgtaactta tacactcgca
    11341 agagtgaaaa attttttgcg gagagtttga tcctggctca ggatgaacgc tggcggcgtg
    11401 cc
//
LOCUS       DAOQJC010000123        11312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2905_length_11312_cov_8.987919, whole genome shotgun sequence.
ACCESSION   DAOQJC010000123 DAOQJC010000000
VERSION     DAOQJC010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11312
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2905_length_11312_cov_8.987919"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="PLE39_15420"
     CDS             <1..1194
                     /locus_tag="PLE39_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69770.1"
                     /translation="DEFAGVPGQYVVVAADYNFAFMVLPVFKIEPSMIHLASFRQGVV
                     EQFYATRAINTPIILAGPPTNTPLPTDTPTNTATFTITPSPTITPSPTLTSTSTPTQT
                     FTPSRTPTKTLTPTFTKTPTKTYTPTITRTPTSTPTPSCASIFIDRTQFNGDSFEARV
                     RNNNVATAYLTETELTWDPSPLTGGRYFDFTKWNGTTYYNPANTANTNSPIVTSSTPP
                     ALDLPGSGGRYTWDADFSDNNFQGLYTVVMTFTFPGWGDCILTGSVANYTPTPSNTPT
                     ITPSPTITRTPTLTPAPSPVPTSTVTIPPTITLTPSRTPSPTITRTPTNTQPATSTPT
                     KTNTPTITRTPTGTFTPTATATRTATPTNTATPTKTNTPPPTLTPSKTPTKTPTPTPT
                     FDPDA"
     gene            1729..3576
                     /gene="glmS"
                     /locus_tag="PLE39_15425"
     CDS             1729..3576
                     /gene="glmS"
                     /locus_tag="PLE39_15425"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNE69771.1"
                     /translation="MCGIVGYVGPRDAVSIILNGLKRLEYRGYDSAGVAVINSNQIEV
                     RRDAGKLSQLIDLVVKSPLTGAPGIGHTRWATHGAPSARNAHPHVGNSGKVVVVHNGI
                     VENFLELKDELVSEGVNFLSDTDTETIVHLAEHYQAANPGAGFVEAARRTFQKIEGAN
                     VVVLMSADEPDKIVTARIGNAGGVVIGLGENENFIASDIPAILEHTRKVIFLESRQMA
                     IVTREGVRIETLEGVEVKPEIHTIAWDAVAAEKGEYRHFMQKEIHEQVRALTDTLAGR
                     VDFKEGRIRLPELNLTPELAKRIQRIYITACGTAAYAGMVGKYLIEKIARVPVEVVIG
                     SEFRYSDPIVDENTVILAISQSGETADTLAAMEEGRRKGGIIWSIVNAVGSQAMRVSN
                     GTIAMQTGPEIGVASTKAFTAPLVDQYMLAILLADMRGVIDEKTRKALVADLRLVPDL
                     AGRVLDTEPEVEKVAHALKDIKGCLYLGRGINMPIAYEGALKLKEISYIHAEGYPAGE
                     MKHGPIALIDEEMPVLCIAPKDPWHEKMISQIQQAKARGGMVIAVATEGDELVPGMAD
                     HVLWIPEAPWMLSPIITVLPLQLLAYHIATIRGLDVDQPRNLAKSVTVE"
     gene            3653..4096
                     /locus_tag="PLE39_15430"
     CDS             3653..4096
                     /locus_tag="PLE39_15430"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HNE69772.1"
                     /translation="MPGENMRPDWDSYFMKIAYAVSERSTCDRAFVGCVLVLEKRILT
                     TGFNGSPAGQEHCDEVGHLMVEGHCVRTIHAETNAIIQAALHGVSTKGATCYVTHLPC
                     IQCTKALINAGITRIVYSTAYRTDENALNFLKAANIEVEQKEFRP"
     gene            complement(4130..4978)
                     /gene="murI"
                     /locus_tag="PLE39_15435"
     CDS             complement(4130..4978)
                     /gene="murI"
                     /locus_tag="PLE39_15435"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNE69773.1"
                     /translation="MMNLQNRQDAITSPIGVFDSGVGGLSVLRSIREQMPEESVLYFG
                     DQGHVPYGPRSMGQIQSFSAGITRFLLDHGSKLIVVACNTASAAALTYLRESFPGVSF
                     VGMEPAVKPAAETTKTGRVGVLATPATFQGALYASVVERFGAGVELFQHTCPGLVSQI
                     EKGELDSAATRAILEDALHPMLEKNIDTVVLGCTHYPFVIPLIQEVVGGAERVRVIDP
                     APSVAKQAKRLLEAAGMKNPSGRRASIRFFTSGDVESMKSMLPVLLGEMGDVELAVWQ
                     NDEEVK"
     gene            complement(5001..5714)
                     /locus_tag="PLE39_15440"
     CDS             complement(5001..5714)
                     /locus_tag="PLE39_15440"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNE69774.1"
                     /translation="MPKPLIGITTRNGKDADGHPLTALQHSYINAIVQAGGMPILIPS
                     MLSEEDFTGLYSLLAGILFTGGGDVSLEYFNGSDHPRIGEVDKRRDTTEITLMRAAVN
                     DGKPLLGICRGAQVMNVALGGTLYTHIHDQVKGALDHDYPGDLRKVIVHPVNVDESTR
                     SAEIFGETLLHVNSLHHQGLKDIAPGLRVAGHAPDGLVEVVEIPDHPYAVAVQWHPEW
                     LTDQPAMQKLFKSFVDAAG"
     gene            complement(5797..6144)
                     /gene="rplS"
                     /locus_tag="PLE39_15445"
     CDS             complement(5797..6144)
                     /gene="rplS"
                     /locus_tag="PLE39_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNE69775.1"
                     /translation="MTDFIKAVEAKPNEKIPQMSSGDTVSVHVKIKEGDRERIQEFKG
                     IVIRLRKGGNDASVTVRRMATNGIGVERTFLLRSPRIDKVVVERHSKVRRAQLYFMRG
                     RTGKSARLKQKFD"
     gene            complement(6232..7470)
                     /gene="fabF"
                     /locus_tag="PLE39_15450"
     CDS             complement(6232..7470)
                     /gene="fabF"
                     /locus_tag="PLE39_15450"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNE69776.1"
                     /translation="MTERIVITGMGTVNPLGLNVKETWSNMVAGVSGVAPITLFDSGP
                     LQVHIAAEVKGFKPEEYMDAKEARRRDRFEQFAITAAKEALFQSGLQVTEKNAGRIGV
                     IISSAIGGLHSLQEATLTNYKEGPKRVSPFLIPMLMPNGGAGMAAIEFGIKGPCFSVA
                     SACASGADGIGTALMMLHTGMIDAALAGAAEATITSTGVAAFDRVGAMSRRNDDFSMT
                     PQPFDKNRDGLVMGEGAGVLVLEREADAKARGANILAELAGYGATADAYHVTAPHEHG
                     EGGTAAIMMALSSAKANIQDVGYINAHGTGTLLNDQAETRAIKAAFGELAYKVPISST
                     KSMTGHMMGATGALEAIICVQAVRDGILPPTIHYETPDPECDLDYIPNKAREADISLA
                     ISNAFGFGGHNAVLAVRKYA"
     gene            complement(7608..8162)
                     /locus_tag="PLE39_15455"
     CDS             complement(7608..8162)
                     /locus_tag="PLE39_15455"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69777.1"
                     /translation="MILNSKRLIIRNFKDSDIESFLLYRNIPEVAKYQGWKLPYPREA
                     AVQLVDEMRNIDSPQAGRWLQLMLELRDTHEVIGDIGIRLKREDPRQAVIGFTLAPAH
                     WRKGYMTEAVVTILGFLFDVLNLHRVSADCDTENIASWRTLEKAGFRREAHFVENYPM
                     GDFYASEYIYSILQREWQAGKTPG"
     gene            complement(8164..8631)
                     /locus_tag="PLE39_15460"
     CDS             complement(8164..8631)
                     /locus_tag="PLE39_15460"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNE69778.1"
                     /translation="MNSSNLPTIFSITESEIDLNDLLARITLTSTGAAAIFTGMVRGV
                     TTRGDAHETEYLEYESYVPMAEAKMKQVADEIRAKWPVIEGIAVVQRIGKLYPMTPTV
                     LIACTAAHRDTGVFDAARYGIDRLKEIVPVWKKEVGPNGEEWVEGDYIPKPGE"
     gene            complement(8640..8888)
                     /locus_tag="PLE39_15465"
     CDS             complement(8640..8888)
                     /locus_tag="PLE39_15465"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNE69779.1"
                     /translation="MNHIKLLFFATIRDRAGVKSLEMDIPADLTIQGLKDKLSVEYPS
                     LKESMQSVLITINREYAFDEAIVPPNAEIGMFPPVSGG"
     gene            complement(8890..9264)
                     /locus_tag="PLE39_15470"
     CDS             complement(8890..9264)
                     /locus_tag="PLE39_15470"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNE69780.1"
                     /translation="MNNMEYSPVNFQEKFSRFSEQWSPKIIAQLNDYHLKLAKVQGEF
                     VWHDHPETDEVFIVIKGHLDILFRDGKVSLTEGEMFVVPKGVEHKPVAENECHILLIE
                     PAGTVNTGDVTDKLTAPNNVWI"
     gene            complement(9257..9733)
                     /gene="moaC"
                     /locus_tag="PLE39_15475"
     CDS             complement(9257..9733)
                     /gene="moaC"
                     /locus_tag="PLE39_15475"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNE69781.1"
                     /translation="MSKLTHLDEQGRAHMVDVGAKPDTERTAIARGEIHMKKETFELI
                     RDGQVKKGDVLTVAQIAGISASKRTSELIPLCHPLFLSKVDVTLVLEESLPGVVITAT
                     AKTTGKTGVEMEALTAVSVAALTVYDMAKAAEKTMRIQNIRLVEKHGGQSGDVVNE"
     gene            9768..10541
                     /locus_tag="PLE39_15480"
     CDS             9768..10541
                     /locus_tag="PLE39_15480"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNE69782.1"
                     /translation="MENLLGHQISGYKVQTPVYEGPLDLLLSLIERAELNITAISLAS
                     VTDQYLSYLHGLEQMKPDEISSFLVIAAKLVQIKSEALLPRPIEREPGEEDPGVELVE
                     LLISYKRYKEIAGWLAERQENNLRTHLRVAPPPKVEAKLDLSNLTLEGLLAAAEAGIA
                     RGKEKKPLATVISTPKVTIREKIDYITKTLKNIQRMSFSGLITDKATRVEIVVTFLAL
                     LELVKRYRVTAKQDVLFGDIEFERSEDWKDDEEIDIEFE"
     gene            10646..11227
                     /locus_tag="PLE39_15485"
     CDS             10646..11227
                     /locus_tag="PLE39_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69783.1"
                     /translation="MKKIFYLLPVFFTLLACGAQPSPTQDISNIVNATLTAVAQNSPQ
                     METPQTAPTQDISSAVNATLTAVAQNNPQGSEQQPVVAQVTGSLRIEQEQGSGAICTA
                     GTTYFVYAEFSSNGPATAQYQIYITDASGQVPNGTFEGYNAPQAEGTLTFDSAKTETL
                     TWRITGPYQYPEALTIRGNLNGQSLQSVPVACQ"
BASE COUNT         2894 a         3152 c         2689 g         2577 t
ORIGIN      
        1 gatgagtttg caggcgtccc cggtcaatac gtggtggttg ccgcagatta caattttgcc
       61 ttcatggtgt tgccagtttt caagattgaa ccctccatga ttcacttggc ttccttccgc
      121 cagggcgtgg tggagcagtt ctatgcaact cgcgccatca acacgccaat cattctggct
      181 ggtccgccaa caaacacacc acttcccacc gataccccga ccaatacagc aacctttacc
      241 attacaccgt caccgaccat tactccttcg ccaacactca ctagtacctc tacaccaaca
      301 cagacgttta caccatcgcg gacaccaacc aaaactctga cgcctacgtt cacaaagacg
      361 ccaaccaaga cctatacgcc aaccattaca aggacgccta cttcaacgcc aacaccttcc
      421 tgtgccagca ttttcattga ccgtacccag ttcaatggtg acagttttga agcacgtgtc
      481 cgtaataaca acgtcgcaac agcctacttg actgaaaccg aactaacatg ggatccatcc
      541 cctctgacag gcggaaggta tttcgacttc accaaatgga acggcaccac ttattacaac
      601 ccggcaaaca ccgccaatac caactccccc attgtgacca gttccacccc gcctgcactt
      661 gatcttccgg gcagtggtgg tcgctatact tgggatgccg acttcagcga caataacttc
      721 cagggtttat acacggttgt gatgacattt accttcccgg gctggggaga ctgtattctg
      781 actggttctg tggccaacta cacacccacc ccgtctaata cacctaccat cacacccagc
      841 ccaacgatca cacgcactcc cacccttacc ccggctccgt ccccggtccc gaccagtacg
      901 gtaacaatac cgccaactat tacccttact ccgtcacgta caccgtcgcc gaccattaca
      961 cgcacgccca ccaataccca accggccacc agcacgccga cgaagaccaa cacgccgacc
     1021 atcacccgca ccccaacagg gacgttcaca ccgactgcta cggctactcg tacggccaca
     1081 ccgactaaca cggccacacc gacaaagaca aatacaccac cgcccacctt aacaccgtct
     1141 aaaacaccaa ccaagacgcc aacgccaacg ccgacattcg atccagatgc ttaattactg
     1201 atgaacaaaa atatcgccga ccattttcag gtcggcgata tttttacggt ataatcccgc
     1261 caacctatga aaggaggtgc atcgacaaaa caaaacggga aagacagcgc atggactttg
     1321 ttttttggga gatgggcttc attcggtatc cagttggaaa aaaacaaagt tgacgaggac
     1381 gagggttctc cattgcgaaa tggagctaat tctccaaggc ctgatacacc aaaaacataa
     1441 aaggtgtttc aacctataag agaaaaaaat cgaacgatca gcggaagccc tccgggtacg
     1501 ccatacccgt cacatctttc cctccaaaag ataactttgc taataacacc ctctgagcga
     1561 tcacagggtc aaaaagcaag ggattggcga aaacaatctg tcattgcaag ggcgtgaatg
     1621 ccttttccct gatcagcgat ctcccgttca agagattgct tcttcgggga aggaacttgc
     1681 attgcaatga catccatcaa actaatacta atcggaggaa cccctactat gtgtggaatc
     1741 gtcggatatg tcggaccgcg tgacgcggtg tcgatcattc tcaatggcct gaaaaggctt
     1801 gaataccgcg gctatgattc ggctggagtc gctgtcatta acagcaacca gatcgaagtt
     1861 agaagagacg cgggcaaatt gtcgcaatta attgaccttg tggtcaaatc cccgctcacg
     1921 ggtgcgccgg gcatcggtca tacacgttgg gcaacccacg gcgcaccatc ggctcgcaat
     1981 gcgcatccgc atgtgggcaa ttcgggcaag gttgtggtgg tgcataacgg catcgtcgaa
     2041 aactttctcg aactcaaaga cgaactcgtc agtgaaggcg taaatttcct ttcagacacc
     2101 gacaccgaaa ccatcgtgca tctggcagag cattaccagg cggccaaccc aggcgctggt
     2161 tttgtggaag ccgcccgccg cacctttcaa aagatcgaag gggccaatgt ggtagtgctc
     2221 atgtctgctg atgagcctga taagatcgtg actgcccgca tcggcaatgc aggcggcgtg
     2281 gtgatcggcc ttggtgaaaa cgaaaacttc atcgcctctg atatccccgc cattttggaa
     2341 cacacccgca aagttatttt tcttgagtcg cgtcaaatgg cgatcgtcac ccgagaaggc
     2401 gtacgcatcg aaacacttga aggagtggag gtcaaacctg agatccacac catcgcgtgg
     2461 gatgccgtgg ccgctgaaaa aggcgagtac cgccacttca tgcaaaagga aatccacgag
     2521 caggtgcgcg cactgaccga cacacttgca ggtcgtgtgg atttcaaaga aggccgcatt
     2581 cgcctgcctg aattaaatct cacccccgaa ctcgccaaac gcatccaacg catttacatc
     2641 acagcttgtg gtacggccgc ttacgcaggc atggttggca aatacctcat cgaaaagatc
     2701 gcacgcgttc cagtggaagt ggtcatcggc tccgagttcc gttacagtga ccccatcgtg
     2761 gacgaaaaca ccgtcattct cgcaatttcg caatctggcg aaaccgccga tacactcgcc
     2821 gccatggaag aaggacgacg caagggcggc atcatctgga gcattgtcaa tgcggttgga
     2881 tcacaagcca tgcgcgtttc aaatggaacc atcgcgatgc aaactggacc tgaaatcggg
     2941 gtggcttcaa ccaaggcgtt tacagcgccg ttggtggatc aatacatgct cgcgatttta
     3001 ctggccgata tgcgcggcgt catagacgaa aagactcgca aggcactcgt ggctgacttg
     3061 cgccttgtcc cagaccttgc tggtcgcgtg cttgataccg aacccgaggt tgaaaaagtg
     3121 gcacatgcct tgaaagacat taagggctgc ctctaccttg gacgcggcat caacatgccg
     3181 atcgcgtacg aaggcgcgct caaactcaag gagatctcct acatccacgc tgaaggttat
     3241 cctgcgggcg aaatgaaaca tggtcccatc gccctcattg atgaggaaat gccggtgttg
     3301 tgcattgccc ccaaagatcc ctggcatgag aaaatgatct cacagatcca gcaggccaag
     3361 gcccgtggcg gcatggtcat tgcagtggct actgaaggcg atgagctcgt cccaggcatg
     3421 gctgaccacg ttttgtggat ccccgaagcg ccgtggatgc tctcccccat cattaccgta
     3481 ctcccattgc agttgctggc ttatcacatt gccaccatcc gcggtttgga tgtggatcaa
     3541 ccccgcaatc tcgccaagag cgtaacagtg gaataaaagt aaaaagacct ggcaggtttc
     3601 aaagcaaaga acttcgtttg atctttgtcc tgaaaacctg tcaggtctga taatgcctgg
     3661 agaaaatatg cgccccgatt gggattccta tttcatgaag atcgcttatg cggtctccga
     3721 acgtagcact tgtgaccgcg cttttgtagg ttgtgtgctt gttttggaaa aaagaattct
     3781 aactaccggc ttcaacggct cccccgccgg gcaggaacat tgtgatgaag tcggtcacct
     3841 catggtggaa ggtcattgcg tccgcacgat tcacgcagaa acaaacgcca tcattcaagc
     3901 tgcgctgcat ggtgtctcca ccaagggagc aacctgttac gtcacacatc tgccttgcat
     3961 tcaatgcaca aaggcgctga tcaatgcagg catcacccgc atcgtgtaca gcactgccta
     4021 tcgcaccgat gaaaacgcgt tgaattttct caaggcggcc aatattgaag tggaacaaaa
     4081 agaatttcga ccctagcaat aaaaagactt cggaaattcc gaagtctttt tatttaacct
     4141 cttcgtcatt ctgccacacg gccagttcta catcgcccat ttcccccaac aacacaggca
     4201 acatggattt catggattca acatccccgg aagtaaaaaa gcggatggat gcccgacgcc
     4261 ccgaaggatt cttcatccct gccgcttcca acaaacgttt ggcctgcttc gccaccgatg
     4321 gggcaggatc gatcactcga acacgttccg cgccccccac cacctcctga atcagcggta
     4381 tcacaaatgg atagtgtgtg catcccaaca ccacggtgtc gatatttttc tcaagcatgg
     4441 gatgaagcgc atcctctaaa atggcacgcg tagcagcaga gtccaattcc cctttttcaa
     4501 tttgactcac caggcccggg caggtatgtt gaaacagctc aacacctgcc ccaaaccgct
     4561 ctacaaccga ggcatacaat gcaccttgaa aggttgccgg ggtggctaat acccccaccc
     4621 tgcccgtttt ggtcgtctcc gcagcaggtt tcacggcagg ctccatgccg acaaaagaaa
     4681 caccgggaaa actttcgcgc agataggtca atgcggcggc ggaggcggtg ttacaggcca
     4741 cgacaatcaa ttttgaacca tggtccagca aaaagcgggt gatgcctgca gaaaaacttt
     4801 gaatctgccc catcgagcgc ggaccatatg gaacgtgtcc ttgatcgcca aagtataaaa
     4861 cggattcctc tggcatttgc tcgcgaatgg aacgcaggac ggataatccg cctacgcctg
     4921 agtcaaaaac accgatgggg gaagttattg catcctgcct gttttgtaaa ttcatcattc
     4981 tgaattcatc gttctttgat tcatccagcc gcatctacaa atgacttgaa taatttttgc
     5041 attgcaggtt ggtctgtcag ccattcgggg tgccattgca cagccacggc ataaggatgg
     5101 tcggggattt ccacaacctc caccaggcca tcaggagcgt ggcctgctac gcgcaagccc
     5161 ggggcgatat ctttcaagcc ttgatggtgc aagctgttga catgcagcag ggtctcacca
     5221 aaaatttcag cggaacgggt gctctcgtct acatttacgg gatggacaat taccttgcgc
     5281 agatcaccgg ggtaatcatg gtcaagcgca ccttttacct gatcgtggat atgggtatat
     5341 agtgtgccgc ccaaagccac gttcataact tgagcgccgc ggcaaattcc caacaatggc
     5401 ttgccatcgt taacggctgc gcgcatcagg gtgatctcgg tagtatcccg tctcttatcc
     5461 acttcaccaa ttctcgggtg gtcagaaccg ttgaaatatt ccaaagaaac gtccccgccg
     5521 ccggtgaaga gaatgccggc aagcagggaa tacaatccag taaagtcttc ctcagaaagc
     5581 attgagggga tcaaaatggg cattccaccc gcctgcacaa tggcgttgat gtaggaatgc
     5641 tggagcgcag taagtggatg tccgtcggca tccttgccgt tgcgggtggt gatgccgatc
     5701 aatggtttgg gcatggtaca actccttgaa aacaaaaaga ccctaaaggt cttgtgaacc
     5761 tttagggtcc aagaaccctg gtggtgactg ttgcttttag tcgaatttct gcttgagacg
     5821 ggcgctcttg ccagtgcggc cacgcatgaa gtaaagctga gcgcggcgga ctttgctgtg
     5881 gcgttcaaca actaccttgt cgatgcgcgg ggagcggagc aggaaggtgc gctctacgcc
     5941 gatgccgttg gtggccatac ggcgaacagt aacggaagcg tcattgccgc ccttgcggag
     6001 gcgaataacg attcccttga actcctgaat acgttcgcgg tcgccttcct tgattttgac
     6061 atgaacgctg accgtgtcgc ctgaactcat ttgcgggatt ttctcgttcg gtttggcctc
     6121 aacggcctta ataaagtctg tcattttacg ttcttcctta cgttttattg gttgtttatg
     6181 aaatgcgaat gccgagtata gcacgcattt tctgaaccta caagcctatt ttcaggcgta
     6241 tttccgcact gccagcacgg cgttgtgacc gccaaagccg aaggcattgc tgatggccag
     6301 cgaaatatca gcctcacgag ccttgttggg gatgtaatcc aggtcacatt cggggtcggg
     6361 ggtttcgtag tggatggtgg gaggcaaaat gccatcacga accgcctgca cacagataat
     6421 tgcctccaat gcgccggtgg cacccatcat gtgccctgtc atggatttgg tggatgagat
     6481 cggaacttta tatgccagtt cgccaaaggc agctttaatg gcgcgggttt cggcctgatc
     6541 gttcaaaagc gtaccagtac catgggcatt gatatagcct acatcctgaa tattcgcctt
     6601 tgcagacgac aacgccatca taatggcggc cgtaccaccc tctccatgtt cgtgtggagc
     6661 agtgacatga taagcatcag ccgtagcacc ataaccagcc aactcagcaa ggatgtttgc
     6721 accgcgagcc ttcgcatctg cctcgcgttc aagcaccaac acccccgccc cttctcccat
     6781 caccagcccg tcacggtttt tatcaaaggg ctggggagtc atggagaaat cgtcattacg
     6841 gcgagacatg gcccccacac ggtcaaaagc agccacacca gtggaggtga tggtcgcctc
     6901 cgcggcgcct gccagagcgg catcgatcat gccagtgtgc agcatcatca atgccgtacc
     6961 aattccatct gcaccagaag cgcaggccga agccaccgaa aagcacggac ctttgatgcc
     7021 gaattcgatc gcagccatcc cagcaccgcc gttcggcatc agcatgggaa tcaggaatgg
     7081 gctgacccgt ttcgggcctt ccttgtaatt tgtgagtgtg gcttcctgaa gcgagtgcaa
     7141 accgccaatg gcagaagaaa taatcacgcc aatgcgccca gcattttttt ctgtcacctg
     7201 caagcctgat tgaaaaagag cctctttggc ggcggtgatc gcaaattgct caaaacggtc
     7261 acggcggcgc gcctctttgg catccatata ttcttcgggc ttgaaaccct tcacttctgc
     7321 ggcaatatga acttgtaacg gccccgaatc aaacagggtg atcggcgcaa cccctgagac
     7381 ccccgccacc atattgctcc aggtctcctt cacattcaaa ccaagtggat tcacagtccc
     7441 catgcctgta atcacgatac gttcagtcat tcttcctcca gataccttga cataataaaa
     7501 ttccagctga ctggcttcat ataaaacccc aaaaactgtt atccgtcacg cggatgaaaa
     7561 cccgtatccc gccttactgc ggcctgcttc cgttgaaaaa acattcccta cccgggggtt
     7621 ttccctgcct gccactcacg ctgcaatatt gagtaaatgt attcactggc ataaaaatca
     7681 cccattgggt aattctccac aaaatgggcc tcacggcgaa agccagcctt ctccagcgtg
     7741 cgccaggaag cgatattctc cgtgtcacaa tcggctgaaa cccgatgcag gttgagcaca
     7801 tcgaaaagaa atcccagtat ggtgacgacc gcctcagtca tatatccttt gcgccagtgt
     7861 gcaggagcca gagtaaagcc gataaccgcc tgccgtggat cttccctctt aaggcgaata
     7921 ccgatatcgc cgatgacttc atgggtatct cgaagctcga gcatcaactg caaccaacgc
     7981 cccgcctgcg gactatctat attccgcatc tcatccacca actgcaccgc cgcttcgcgt
     8041 ggatagggca gcttccatcc ctgatatttc gccacttcag gaatattgcg atacaaaaga
     8101 aaggactcaa tatctgagtc cttgaaattt cgaatgatca atcgttttga attcaaaatc
     8161 atatcattct ccaggcttgg ggatgtaatc gccttccacc cattcctcgc catttgggcc
     8221 aacttccttc ttccagacgg gtacgatctc tttcaagcgg tcaatgccat agcgggccgc
     8281 atcgaatacg cccgtgtcac ggtgagctgc ggtacaggcg atcaataccg tgggggtcat
     8341 tgggtacagc ttgccaatgc gctgaacaac ggcaatcccc tcgatcacgg gccattttgc
     8401 acggatctca tcggccacct gcttcatttt ggcctcagcc atcggaacat aagactcata
     8461 ctcaaggtat tcggtttcat gggcgtcacc gcgtgtggtc acaccccgaa ccatgcctgt
     8521 aaaaatagcc gctgcacctg tgctggtgag cgtaatcctt gcaagcaggt cgttcaggtc
     8581 aatttctgat tcggtgatgg aaaaaatcgt tggaaggttg gaagaattca tggttcgggt
     8641 catccaccag agacgggggg aaacatgccg atctcggcat tcggcgggac aatagcctca
     8701 tcaaaggcat attcacgatt gatcgtaatc aagacagatt gcatggattc cttgaggctc
     8761 ggatattcca ctgacaattt gtctttgaga ccttgaatgg tcaggtcggc ggggatatcc
     8821 atttcaaggg atttcactcc cgcccgatcg cggatggtgg caaaaaacaa gagttttatg
     8881 tgattcattt tatatccaga cattatttgg agcagtgagt ttgtcagtta catcgcctgt
     8941 gttgaccgta cccgcaggct cgataagcaa gatgtggcac tcgttttcag caacaggctt
     9001 atgttccaca ccttttggaa caacgaacat ctccccttca gtcagggaaa ccttgccgtc
     9061 acggaataaa atatccaaat gccctttgat aacgataaac acctcatcgg tttcagggtg
     9121 gtcatgccac acaaattctc cctgcacctt ggccagttta agatggtaat catttaactg
     9181 ggcaatgatc ttcggtgacc actgctcgga gaacctggaa aacttctctt gaaaattgac
     9241 gggagagtat tccatattat tcattcacca catcgccgct ttgaccgcca tgtttttcca
     9301 caaggcggat attttgaatg cgcatggttt tttcggcggc tttggccatg tcgtacacgg
     9361 tcaacgcggc gacagagacc gctgtaaggg cctccatttc cacacctgtt ttgcctgtgg
     9421 ttttggcggt ggctgtgatg accactcccg gcagggattc ttcgaggacg agggtcacat
     9481 ccactttcga aaggaagagc ggatgacata acggaatcag ctccgaggtc cgttttgagg
     9541 cgctaattcc tgcgatctgg gctacggtca gcacatcccc ctttttgacc tgcccatcac
     9601 gaatcagttc gaaggtctct tttttcatgt gaatttcacc gcgggcaatg gcggtgcgtt
     9661 ccgtatcggg cttggcaccc acatccacca tgtgcgcacg gccctgttca tcgaggtgag
     9721 taagcttgga catgggcagg attatacact ggtataattc ggccgccatg gaaaacctgc
     9781 taggacatca gatcagcgga tacaaggtgc agacgcccgt ttatgaaggg ccactggacc
     9841 ttttactcag tttgatcgaa cgcgctgaat taaatatcac cgccatttca ctggcttcag
     9901 ttaccgacca gtatttatca tacctgcacg ggctggaaca gatgaagccc gacgaaattt
     9961 cgtcgttcct tgtgatcgcc gccaagctgg tgcagatcaa atctgaagca ttgctgccgc
    10021 gaccgatcga acgtgagcct ggcgaagaag accccggtgt agagttggtg gaattgctga
    10081 tttcatacaa acgctacaag gaaatcgcag gctggcttgc cgaacggcag gaaaataatt
    10141 tacgcacaca tttgcgcgtg gccccgcccc ccaaagtgga ggcaaaactc gacctttcga
    10201 atctaaccct tgaaggtttg ctggccgctg ccgaggcagg cattgcacgc ggcaaagaga
    10261 aaaaaccgct tgccacagtg atcagcacac ccaaagtgac gatccgtgaa aaaattgact
    10321 acatcaccaa gaccttgaaa aacatccagc gcatgtcatt cagcggactg atcaccgata
    10381 aagccactcg ggtggagatc gtggtgactt ttctcgcgct attggaactg gtgaaacgct
    10441 accgtgtgac agcaaaacag gatgtcctct tcggcgacat cgaatttgaa cgcagcgaag
    10501 attggaaaga cgacgaggaa attgacattg agttcgaata atgcagacgg gggaatttgc
    10561 gcaacaaggt ttgttttttc tcgcatgctc taaaaagcgg gaaactatca agaatcgaat
    10621 acgtcaaatt tacccaggag aaaaaatgaa aaaaatattt tatttgttac ccgtgttttt
    10681 cacccttttg gcttgcggag ctcaaccttc gccgacccag gacatcagca atattgtcaa
    10741 cgcaactttg accgcagtgg ctcaaaacag cccgcaaatg gaaacaccgc agaccgcacc
    10801 gacccaggat atcagcagcg ccgttaatgc aacccttact gccgtggctc aaaacaatcc
    10861 acaaggcagc gaacaacaac ccgttgttgc tcaagtcaca ggttcactcc gaatcgagca
    10921 agaacaaggg tcaggagcca tttgcacggc gggcacaaca tattttgtgt atgccgaatt
    10981 ctcatccaat gggcctgcca ccgcgcaata tcaaatttac attaccgatg ccagcggaca
    11041 ggtacctaat ggcacttttg agggctacaa tgcgccgcaa gctgaaggca cattgacctt
    11101 cgatagcgcc aaaacggaaa cattgacctg gcgcataact ggtccctatc aatatccaga
    11161 ggcacttacc atacgcggaa acttaaatgg acaatctttg cagagtgtcc ccgttgcctg
    11221 ccaataacat cctgacattc aaaaatacac ctcttccatt caggaagagg tgtatttttt
    11281 taattgcttc tctgactaaa tttccccggc gg
//
LOCUS       DAOQJC010000124        11238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2930_length_11238_cov_9.861307, whole genome shotgun sequence.
ACCESSION   DAOQJC010000124 DAOQJC010000000
VERSION     DAOQJC010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2930_length_11238_cov_9.861307"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLE39_15490"
     CDS             complement(<1..261)
                     /locus_tag="PLE39_15490"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE69784.1"
                     /translation="MTSKTLPQSQHFTYHELAEGIWAAIVIPTGLAASNSGIVDLGDH
                     TLIFDTTLSPASAVELRKTAENLTGRAVTYVLNSHWHQDHVFG"
     gene            complement(392..631)
                     /locus_tag="PLE39_15495"
     CDS             complement(392..631)
                     /locus_tag="PLE39_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69785.1"
                     /translation="MSSEEKQIVHRVVENFIRTGNATDEQVKVTCLPVNKTSFVEQTG
                     GDGRIILLDEYKLDGKVIWASYSARSGTVYLSLKK"
     gene            complement(633..1130)
                     /locus_tag="PLE39_15500"
     CDS             complement(633..1130)
                     /locus_tag="PLE39_15500"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNE69786.1"
                     /translation="MNFQEGDPVVHWTYGFGQVVRLEERELAGAKTLYYAVQVRDLTV
                     WVPDDGKLESRLRPPTPETEFKKMINILSSPGKPLPEDRHERKTRLQEMQKNGGTEAL
                     CHIIRDLHTYQQVKSLNDNDQVILKQSQTALLGEWGFVFSISTAQAEIELHRVLAPKT
                     HEIKE"
     gene            complement(1151..1630)
                     /locus_tag="PLE39_15505"
     CDS             complement(1151..1630)
                     /locus_tag="PLE39_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69787.1"
                     /translation="MINLNISALIYQPIKKVFDFVCTPENDFQWQYGTLASTTLSTGT
                     ENIGTIFRSVGNLMGHRIQSTFEVTEFEPNKKYGFKSLSGPLLSQTVYTFEIDESSTK
                     VSISTQANVVNFFQSDEGILEKKMKKQLKENLAMLKTLLEVRRVTAPLNADTQPSKT"
     gene            complement(1649..2056)
                     /locus_tag="PLE39_15510"
     CDS             complement(1649..2056)
                     /locus_tag="PLE39_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69788.1"
                     /translation="MDKKNKNPLLAGLINVLIPGASQLYVNNDRARFFRSFLMGAGAF
                     VVAIMLGNLIQNTRGYSLPQGVCMGSALTLVAVILFISGLRVAGERNSQTDNAAYYKS
                     MRRDGYAAKQTAANKAQTEGSASEQKDAEPKQK"
     gene            2388..3413
                     /locus_tag="PLE39_15515"
     CDS             2388..3413
                     /locus_tag="PLE39_15515"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNE69789.1"
                     /translation="MRKTRKYATISDVAQHAGVSIATVSRVLTGNTPVIPETAERVRA
                     AIAELRFVPRTAARTLASRKTNTIGLILSEIGGAFFPHLLKGIEAEVRAAGYELLIHS
                     TQGMRQHQHRAVGEHNTDGLLVFTSSLELDELKRLHSMNFPLVLMHQTPPADLNIPVI
                     TIENKDGAEMLVGHLIEKHGRRRIVYMQGPEGHEDSHWRERGYREALEKRNIPFDPEL
                     VVDGAFDEEQACTTIQQMLMDGIEFDAVFAGDDDAAMGVYRALKLAGRIIPDDVAVVG
                     FDDIQFARYISPALTTIRAPIEEVGREAVRQLIHLLNGQQAQSLVLMRTELVIRESCG
                     CALSNRL"
     gene            3501..4874
                     /locus_tag="PLE39_15520"
     CDS             3501..4874
                     /locus_tag="PLE39_15520"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNE69790.1"
                     /translation="MKKNIFKLLAFLVLTSLLLTACGGGGAAPAEKAKVTIFVGFGTG
                     TDPDQVTAQEALAKKFNESHTDIEVEFLIVPVEESTERYLAMVAGGNAPQLVGPHGIS
                     TIAKFKDQWEDVTPYIEKDKYDMSDFYGPSTTLNKYPDLNAGLPLGLYPSFIFYNKDA
                     FDAAGLEYPTHDYADTSWNIDSMKEMALKMTLDANGNTPADSGFDPATIKQWGYADTW
                     TDFRGQMTMFGADTKGRPTSDDYKTAQVNSAEWLFGAQWLHDGVFKDHFIPDAAGQGA
                     IDAASGDPFGGGHVAMWYSHTWYMSEGLNDLPFKYDIAPLFYNQNGERISRIHADLFT
                     MPKTAKNKDAAWEVMKWLVAPEQIVDVCKVYGCLPARQSSQEAYLNYMKERYPGIDYD
                     VIFTAIDHLDNPNHESWVPEWGKVNDTLNNTQTTLYTDPEADVEALMNTTNEDVQKIL
                     DEYWASK"
     gene            4950..5915
                     /locus_tag="PLE39_15525"
     CDS             4950..5915
                     /locus_tag="PLE39_15525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNE69791.1"
                     /translation="MKPANTISLARREALAGYAFIIPWIIGFLVFTIGPMLASLYFSF
                     TDYNIVDPPRWVGLENYVRVFSGIPEYLRTGDTRQLGDPIFWKSLQVTIYFASLALPL
                     NLLFSFLLAVLLNQKVPGVNIWRTIYFLPSIIAGVAVTLLWIRILNPRMGILNPFLQN
                     VLGVDNPPGWLQDPNWAIPALVIMSLWGVGGSMIIYLAGLQGIPTDLYDAAKVDGANA
                     WQRFWNVTFPMMTPVLFYNLVLGMIGTFQYFTEVYVATSGTGGPQRATLFYNLYLYQN
                     AFRYFDMGYASTLAWILFIIVLVLTALIFRSSEMWVYYEGQLRGK"
     gene            5915..6973
                     /locus_tag="PLE39_15530"
     CDS             5915..6973
                     /locus_tag="PLE39_15530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNE69792.1"
                     /translation="MKRIIDLFSWLFKSIKGHEFLFKGITTLVLAAGAVVIMIPLMFM
                     ISTSLKDPGQLRSDANTLIPRKPRAVELNGEKFNLYQVTVDGVSLEMAMIKKAPGGMA
                     FFADPANPEELIELNLAEQTALYDIDIHWENYPEALNAMPFFRYLGNTLVVTFVGMAG
                     MLFSSSLVAYGFSRFRARWLNVLFLVLLSTIMLPSQVRLIPIYIFFQKLGWVNTLLPL
                     IVPQFFANAYDVFLLRQFFMTIPLELDDAAKIDGANPLQVLLYIILPQARPALVSVGI
                     FHFLYAWNDFYEPLIFLHSRDNWTLAVGLQTFDALYTINTHLIMAVSVLMIFPPILLF
                     FFSQKIFTQGVVFSGVKG"
     gene            7052..8374
                     /locus_tag="PLE39_15535"
     CDS             7052..8374
                     /locus_tag="PLE39_15535"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HNE69793.1"
                     /translation="MTNYTFPHNFLWGAATAAYQVEGAWNEDGKGESIWDRFSHTPGK
                     ISDQTTGDVACDHYHRYEQDIALMRRLGLKAYRFSIAWTRVLPAGRGRINPKGIDFYD
                     RLIDSLCAANIEPFLTLYHWDLPQALQDEGGWENRDTGLAFADYSALMVKHLGDRVKY
                     WTTFNEPSVVAFDGNLSGEHAPGNRDPRIAYQVAHNLMVAHGLGVQAIRSADSKVQAG
                     IVLNLWMADPATDAPEDIAEARAAWDRSESLFLDPIFKGYYSPAVYDLVGENMPKLQD
                     GDMALISQELDYVGINFYSRNLVSATQEIDTVPGSEYTEMGWEVCAPAMRRMLVKINN
                     EYRLPPIYITENGAAFKDEVSADGKIHDPRRLDYLKQHFIQTRLAMQDGVDVRGYMVW
                     SLMDNFEWGHGFTKRFGLIRVDYETQQRTFKDSGDWYAEVIRTNQIIE"
     gene            8404..9420
                     /locus_tag="PLE39_15540"
     CDS             8404..9420
                     /locus_tag="PLE39_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69794.1"
                     /translation="MKPDTKPETGVSHFANGWPHHFARDLEDILAHHCTYIVHCFSEN
                     EMVFARKRTAQFFKMTRDAGLGCWANPWSVLGLFGGEQFSAFVPRNPDACQILSTGQR
                     APAACPSADETKTAMKLWIDMAIDFGADTIFWDEPHLYIPDWDDLHFASDDAFACFCP
                     RCRVSFQNRFGESMPETLTAEMRLFREDLMVNFLAEMIGYAKDKGAKNAITLLPVEDE
                     ANESLPWDRIADIRGLDIFGTDPYWYLHDKDCKTFVGEQMKKTMDICEPRGLTPHFWA
                     QGFGIPSGRENELETGFSLAVERGAQSIAIWGMHGNSAFDGASENPEKVWDVVGKTFG
                     KLRR"
     gene            9417..9983
                     /locus_tag="PLE39_15545"
     CDS             9417..9983
                     /locus_tag="PLE39_15545"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69795.1"
                     /translation="MKIISLASDQLELIQQAAQILVDAFKDHSPEAWPTLADGLTEVD
                     EMLADDRILRVALDENENVIGWIGGIEQYDGHVWELHPLVVKPGVQGKGIGRALVQDF
                     EEQVRMRGALTVMLGTDDEDNRTSLSNTDLYEEPWKKINEIKNLKRHPFEFYQKMGYR
                     ITGVVPDANGTGKPDILMSKKIQTKQDQ"
     gene            9987..10874
                     /locus_tag="PLE39_15550"
     CDS             9987..10874
                     /locus_tag="PLE39_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNE69796.1"
                     /translation="MEKLFGGIEGGGTKFNCAVGNGPENILAEARFATTTPAETIRQV
                     CDFFMSHVDQLQAIGFGSFGPFDTDPASPTYGYITTTPKPHWGNTNVLGMLREKINLP
                     FAVDMDVAVAGLGEATWGASKNDTCSLYLTVGTGIGGGYIMNGKPLRGLTSLEMGHIR
                     LARDPKLDPFQGACPYHGDCFEGLAAGPAVEKRFGQKGETLPDDHPFWELEAGYIAQA
                     LVNYILCLSPQRIIIGGGVMQKDFMFPAVRRKTQELLGGYISHDVILNHIDEYIVPPA
                     LGGRSGVLGSIALAMNLNA"
     gene            10871..>11238
                     /locus_tag="PLE39_15555"
     CDS             10871..>11238
                     /locus_tag="PLE39_15555"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNE69797.1"
                     /translation="MKKIPNLPIVDLMPFSEIDDKRPALLVTSAPAWNAVKNNLRGLN
                     ITHTIEVTEATTEYWDNLQLPITNHQIEIIYAVGGGLVADAAKYFASKLNLPLVVLPT
                     ALSVDAFITAASGIRKDGCV"
BASE COUNT         2659 a         2839 c         2833 g         2907 t
ORIGIN      
        1 accaaagaca tggtcttgat gccagtgact gttgagcaca taggttaccg cacgtccagt
       61 gaggttttct gcagttttac gcaattcaac agccgatgcg ggcgagaggg tggtatcaaa
      121 aataagggtg tggtcgccaa ggtccacgat ccctgaattg cttgccgcca ggcctgtcgg
      181 aatcacgatc gccgcccaga ttccctcagc cagttcgtga taagtaaagt gctgtgattg
      241 gggaagtgtt ttagaagtca tgggagtgcc tcaacggaac gagtatttgt gcaagatttt
      301 acctatttta taaaccaccc ataaatataa acatccttca aacgatcgga atgaagtttc
      361 ggttgcttga aggaatatat tttcgcctga tttatttttt taggctcaag taaactgttc
      421 cactgcgggc actgtaactt gcccagatca ctttcccgtc caatttgtat tcatccagca
      481 aaatgattcg accatcgccg ccggtctgtt ctacaaaact ggtcttgttc actggcaggc
      541 aggtcacctt gacctgttca tctgtggcat ttccggtgcg gatgaaattt tccacaactc
      601 tgtgtacgat ctgtttttct tctgatgaca tattactcct taatctcatg tgtctttgga
      661 gcaagcacgc ggtgtagttc gatctcggcc tgggcggtgg aaatggagaa aacaaagccc
      721 cattccccga gcagggcggt ttgcgattgt ttcagaatca cctgatcgtt gtcgttgagt
      781 gacttcacct gttggtaggt gtgcagatca cggatgatat gacagagcgc ttcagtgccg
      841 ccgttttttt gcatctcttg cagccgggtt tttcgttcat gacggtcttc cgggagcggt
      901 ttccccgggc ttgagagaat gttgatcatt ttcttgaatt cggtttccgg ggtgggggga
      961 cgtaaccggc tttccaattt tccgtcgtct ggcacccata cagtcaagtc acgtacctga
     1021 accgcatagt acagagtttt ggcccctgcc aattcccgtt cctccaaccg aacgacctga
     1081 ccaaatccat atgtccagtg aacgactggg tcgccttctt gaaaattcat gtggttctcc
     1141 tgttaaaggg ttaggtcttg gatggctgtg tatctgcatt gagaggagcc gtaacacggc
     1201 ggacttccag cagggttttg agcattgcaa ggttttcttt gagttgtttt ttcatctttt
     1261 tttccagtat cccctcatct gattggaaaa aattgaccac attggcctgc gttgagatgc
     1321 tcaccttggt actgctttcg tcgatctcga atgtgtacac tgtctgcgaa agcaacggac
     1381 cggagaggga tttgaagccg tattttttgt ttggctcaaa ttccgtcact tcaaatgtgc
     1441 tttgaatccg atgtcccatc aaattcccga cactgcgaaa gatcgtcccg atattttctg
     1501 tgcctgtgga aagtgtagtg gaggccagag tgccatattg ccattgaaag tcattttcgg
     1561 gggtgcaaac gaaatcaaaa acctttttga tgggttggta gatcagtgcg ctgatattta
     1621 aattgatcat tccactcttt tccggggttt atttttgctt gggttctgca tccttttgtt
     1681 cagatgccga accttcagtc tgtgccttgt ttgcggcggt ttgttttgct gcgtaaccat
     1741 cacgccgcat ggatttgtaa taggcggcat tatcggtttg actgttgcgt tcacctgcca
     1801 ctcttaatcc gctgataaac aagatgaccg ctacgagtgt gagcgcactg cccatgcata
     1861 ctccctgtgg cagggaatac ccacgtgtgt tttgaatcaa atttcccaac atgatcgcaa
     1921 cgacaaaggc tcctgctccc atcagaaatg aacggaagaa tctggctctg tcattgttca
     1981 catataactg acttgccccg gggattaaaa catttatcaa acccgccaga agggggtttt
     2041 tatttttctt atccataaat ttctcctttt catggagaga agacgttcca cagatccccc
     2101 ttccagatcc catgatctct ctctgtcatt gtagcacgtt cacttgcgaa ttgttcgagc
     2161 aattttgtat gccaacctgc aggagccatt gccttgaatg atcggcgata agcagagctt
     2221 cctccggttc aagcattgcg cagtggagtt tttcgcgggt gagattctat tgtttttgta
     2281 atcagtttgg gtgtgagaga agaagcgaat ttttagccaa tttgttgaca tgatgggtga
     2341 gtctcgttat actatgaaag cgttttctga aaacgctttc atagtccatg agaaaaacta
     2401 gaaaatacgc cacaatttcc gatgtagctc aacatgcggg ggtatccatt gccacggtga
     2461 gccgtgtatt gacgggaaat acccccgtga taccggaaac cgctgagcgc gtgcgtgctg
     2521 caattgctga actgcgtttt gtcccacgca cggcggcgcg tacccttgcc agccgaaaaa
     2581 cgaacacgat cggtttgatt ctttccgaaa tcggcggcgc gttttttcct catttgttga
     2641 aaggcattga ggcggaagtg cgtgcagcgg gctacgaatt gcttattcac tccacccagg
     2701 gaatgcggca gcaccaacat cgtgcagtgg gggagcataa taccgatggc ctgttggtgt
     2761 tcacctccag cctcgaactt gacgaactca aacgtctgca cagcatgaat tttccgcttg
     2821 tgttgatgca ccaaacccca cccgctgatt tgaatatccc agtcattacc attgaaaaca
     2881 aagacggcgc tgaaatgctg gtcggtcacc tgatcgaaaa gcatggtcgg cgccgcatcg
     2941 tttatatgca ggggcctgag ggccatgaag attcgcactg gcgcgaacgc gggtatcgtg
     3001 aggcattgga aaaacgtaat atcccattcg accctgaact ggtggtggat ggggcttttg
     3061 atgaagaaca ggcctgcacc accattcagc aaatgcttat ggatggaatt gaatttgatg
     3121 cggtcttcgc cggggatgac gatgcagcaa tgggtgtgta tcgcgccttg aaactggccg
     3181 gccgcatcat tcctgacgat gtggcggttg tcggtttcga tgacattcaa tttgcccgat
     3241 atatctcccc cgcgttgacg acgattcgcg cgccgatcga agaagtgggg cgtgaagctg
     3301 tccgccagct gatccacttg ttgaatgggc agcaggccca aagtttggtt ttgatgcgca
     3361 cagaattggt catccgtgaa tcgtgcgggt gtgcgctttc caatcgttta taaagacggg
     3421 gaggtgttgt cgaagaagat acaggatcac aaggtcaaaa tgaaaaccat ccattccatt
     3481 aatcacccct ggaggagcaa atgaaaaaaa atatattcaa attgctggct tttctcgtat
     3541 tgacatcgct tttgttgaca gcctgtggcg gaggcggagc cgcgcccgct gaaaaagcca
     3601 aggtgaccat ctttgtgggt tttggtaccg gcactgaccc tgatcaggtc actgcacaag
     3661 aggcacttgc aaagaaattc aacgaatcgc acaccgatat cgaagtggaa ttcctgatcg
     3721 ttcctgtgga agaatccact gaacgttatc tggcgatggt ggctggcgga aatgcgccgc
     3781 aattggtggg tccgcatgga atttcaacca ttgcgaagtt caaggatcaa tgggaggatg
     3841 tgactcccta catcgaaaaa gacaaatacg atatgagcga tttctatggt ccttcgacca
     3901 cgctcaacaa gtatccagat ttgaatgcgg gtttgccgct ggggttgtac ccctccttca
     3961 ttttctataa caaggatgcc ttcgatgcgg ctgggttgga ataccccacc cacgattatg
     4021 ccgatacttc atggaatatc gattccatga aagagatggc gctcaagatg accctggatg
     4081 cgaacggaaa cacccctgct gattctggct ttgatcctgc caccatcaaa caatgggggt
     4141 atgccgatac atggaccgat ttccgcgggc agatgacaat gtttggcgct gacaccaagg
     4201 gtcgcccgac cagtgacgat tacaagaccg ctcaggtcaa ttcggcagag tggctgtttg
     4261 gtgcccagtg gctgcatgat ggcgtattca aggatcattt cattcccgat gcagcgggtc
     4321 agggcgcgat tgatgcagct tctggtgacc cgtttggcgg tggtcatgtg gccatgtggt
     4381 acagccatac ctggtacatg tctgaaggtt tgaatgacct gccgttcaaa tatgatattg
     4441 cgccattgtt ctataaccaa aatggcgagc gcatttcccg cattcatgcc gacctgttca
     4501 ccatgcccaa gactgccaag aacaaggacg ccgcctggga agtgatgaag tggttggtgg
     4561 ctcccgaaca gatcgtggac gtgtgtaagg tgtatggatg tctgcctgcg cgccagtctt
     4621 cacaagaggc gtacctcaat tacatgaagg aacgctaccc aggcattgac tatgatgtca
     4681 tcttcaccgc cattgatcac ctcgataacc ccaaccatga gtcgtgggtg cctgaatggg
     4741 gcaaggtgaa tgataccttg aacaataccc agaccacact ctacaccgat cccgaagcag
     4801 atgtggaagc cctgatgaac accacgaatg aggatgtgca gaagatcctc gatgaatatt
     4861 gggcaagcaa atagattgat atgactggat ggaggggcaa ccctccatcc agcttgaaat
     4921 tattttttag tgtttttttc tggaggcgca tgaaaccagc aaataccatc agcctcgccc
     4981 gccgcgaagc cctggccggg tatgctttta tcattccgtg gatcatcggc tttttggttt
     5041 tcaccatcgg cccaatgctt gcatcgttgt atttttcctt taccgattac aacattgttg
     5101 acccgccacg ctgggtggga ctggagaatt atgtccgcgt gttctctgga attcccgaat
     5161 atctaagaac aggtgacacc cgccagttgg gcgacccgat cttctggaag tctttgcagg
     5221 tgacgatata ttttgcttcg ctggccttgc ctctgaatct gctcttcagc tttttgctgg
     5281 cagtgttgtt gaatcaaaag gtacccgggg tgaatatctg gcggaccatt tattttctgc
     5341 cttccatcat tgccggggtg gcggttaccc tgttgtggat tcgcatcctc aacccgcgca
     5401 tgggaatttt gaatccgttt ttgcagaatg tgcttggggt ggataatccc ccgggctggc
     5461 tgcaagatcc aaattgggcc atccctgcat tggtcatcat gagcctgtgg ggggtgggtg
     5521 ggtcgatgat catttatctg gctgggttgc agggtattcc caccgacctg tacgatgctg
     5581 ccaaggtgga tggagcaaac gcctggcaaa ggttttggaa cgtcactttt ccgatgatga
     5641 cccctgtcct tttttataac cttgttctgg gtatgatcgg tacctttcag tatttcacgg
     5701 aggtctatgt cgccaccagc ggcactggtg gaccgcagcg cgccacactt ttctacaatc
     5761 tatacctgta tcaaaatgct tttcgctatt ttgatatggg atacgcctcc accctggctt
     5821 ggatcctgtt tatcatcgtg ctcgtgctga ccgcacttat tttccgctct tcggagatgt
     5881 gggtatatta tgaaggccaa ttgagaggca aataatgaag cgaataattg atcttttttc
     5941 ctggcttttc aagagtatca aggggcatga attcctgttc aaaggcatca ctactttggt
     6001 tcttgcggcg ggagcggtgg tgattatgat cccgctgatg ttcatgattt ccacttctct
     6061 gaaagacccc ggtcagctgc gttcagatgc aaataccctg atcccgcgca aaccgcgggc
     6121 cgttgaactc aacggcgaaa aatttaacct gtatcaggtg accgttgatg gcgtttcgct
     6181 cgaaatggca atgatcaaaa aagcgccggg tggcatggcc ttttttgcag atccagccaa
     6241 ccccgaagag ttgatcgaac ttaatctggc cgaacaaacc gccctctatg acattgatat
     6301 ccattgggag aactatccag aagcgttgaa tgccatgccc tttttccgct atctggggaa
     6361 tacgctcgtt gttacctttg taggcatggc tggcatgttg ttttccagtt cactggtggc
     6421 ttacgggttc agccgtttcc gtgcgcgttg gttgaacgtg cttttcctgg tgttgttatc
     6481 aaccatcatg ctgccctcac aagtgcgcct gattcccatt tacatctttt tccagaaact
     6541 tggctgggta aatacgcttt tgccattgat cgtgccgcag ttctttgcga atgcctatga
     6601 cgtatttttg cttcgccagt ttttcatgac cattccgctt gaattggacg atgccgccaa
     6661 gatcgatgga gcgaatcctt tgcaggtatt gttgtacatc atcctgccgc aggcacgccc
     6721 tgcgctggtt tctgtgggta tcttccattt cctctacgcg tggaatgatt tttacgaacc
     6781 gcttatcttc ctgcattccc gtgataactg gactttggcc gtgggtttgc aaacttttga
     6841 tgccctctac acgatcaata cacaccttat catggctgtg tctgttttga tgatcttccc
     6901 gccgatcctg ctgtttttct tcagccaaaa gatatttaca caaggtgtgg tcttcagcgg
     6961 cgtaaaagga taaatttacc tggccgaaaa ccatcaccgt atacatcact ctgaacatgg
     7021 acgaaaacaa tccacctgaa acctgaatcc tatgacaaac tataccttcc cacacaattt
     7081 cctttggggt gcggcaaccg ctgcgtatca agtcgaaggc gcctggaacg aagatggcaa
     7141 aggcgaatcc atttgggacc gcttcagtca cacccccggc aagatctcag accaaaccac
     7201 gggggatgtc gcctgcgatc attatcaccg ttatgaacag gatatcgccc tcatgcgccg
     7261 acttggcctc aaagcctatc gtttctccat cgcatggaca cgcgtcctgc cagccgggcg
     7321 cgggcgtatc aacccgaagg gaattgattt ttatgaccgc ctcattgatt cgctttgtgc
     7381 ggctaatatc gaaccctttc tcacactcta tcactgggat ttgccccagg ctttgcagga
     7441 tgaaggcggc tgggagaatc gcgacaccgg ccttgccttt gcagattatt ccgccttgat
     7501 ggtgaaacac cttggcgacc gcgtaaagta ttggacaacg tttaacgagc ccagtgtcgt
     7561 cgcgtttgat gggaatcttt ccggggaaca tgctccgggg aatcgagatc cgcgcattgc
     7621 ctatcaggtg gctcataacc tcatggtcgc acatggtctg ggcgtgcaag ccatccgcag
     7681 tgctgattca aaagtgcagg cgggaattgt cctcaattta tggatggcag acccagccac
     7741 cgatgcgccc gaagacatcg ccgaagccag ggcagcatgg gatcgcagtg aatccctttt
     7801 ccttgacccg atcttcaaag gatactactc tcccgcagtg tatgatctgg tgggggagaa
     7861 catgccaaaa ctgcaagacg gcgacatggc cttgatctca caggaactcg attatgtggg
     7921 catcaatttt tactcgcgca atctcgtcag cgccacacag gaaattgaca ccgtacctgg
     7981 ctcggaatac accgaaatgg gatgggaagt ttgtgcgccc gcaatgcgca ggatgctcgt
     8041 gaagatcaat aatgaatatc gcctgccgcc gatctatatc accgaaaatg gtgcggcatt
     8101 caaagatgaa gtcagcgccg atggaaagat ccacgacccg cgcaggctcg actatttgaa
     8161 acagcatttc atacaaaccc gcctcgccat gcaggatggc gtggatgtgc gcggttacat
     8221 ggtctggtcc ctgatggata acttcgaatg gggtcacggc tttaccaaac gattcggcct
     8281 catccgcgtg gattacgaaa cccaacagcg cactttcaaa gactcgggtg attggtacgc
     8341 cgaagtgata cgcaccaatc agatcatcga atagtttaag aaacaaaacc tggaacctga
     8401 aacttgaaac ccgataccaa acctgaaacc ggcgtttccc attttgccaa cggctggccg
     8461 caccactttg cgcgcgatct tgaagatatt cttgcacatc actgcacgta catcgtgcat
     8521 tgtttcagcg agaacgaaat ggtctttgcg cgcaaacgca ctgcacaatt ttttaaaatg
     8581 actcgcgatg cgggccttgg ctgctgggcg aatccctggt ctgtgcttgg gctgttcggc
     8641 ggcgaacaat tttctgcatt cgtgccgcgc aaccccgatg cctgccagat cctttccacg
     8701 ggtcagcgtg cgcccgccgc ctgcccatca gccgatgaaa ccaaaaccgc catgaaactc
     8761 tggatcgata tggcgattga cttcggtgcc gataccatct tctgggatga accgcacctc
     8821 tacataccag attgggatga cctgcacttc gcctcagacg acgcctttgc ctgcttctgt
     8881 ccgagatgta gagtctcgtt ccagaatcgc ttcggtgaat ccatgcccga aacattgacc
     8941 gccgaaatgc gcctcttccg cgaagacctc atggtgaatt tcctcgccga gatgatcggc
     9001 tatgccaaag acaaaggcgc aaagaatgcc atcacgcttt tgcctgttga agacgaagcc
     9061 aacgaatcac tcccatggga tcgaatcgcc gacatccgtg ggctcgacat cttcggtact
     9121 gacccgtatt ggtatttgca cgataaagat tgcaaaacct tcgtcggtga gcagatgaaa
     9181 aagacgatgg acatttgcga acctcgtggg ctgactccgc acttttgggc gcagggtttt
     9241 ggaatcccat cgggcaggga aaatgagctc gaaacaggat tcagcctcgc cgtcgaaaga
     9301 ggcgcgcaat ccatcgccat ttggggcatg cacggaaatt ccgcctttga tggtgcttcc
     9361 gagaatcctg aaaaagtttg ggatgtggtt ggcaaaacct ttggaaaatt gagaagatga
     9421 agatcatttc actggcctcc gatcaacttg aattgatcca gcaagctgca cagattcttg
     9481 tggatgcctt caaagaccat tctcctgaag cctggcccac gttagccgat ggcctgaccg
     9541 aagtggatga aatgctcgct gatgatcgca ttctgcgcgt tgcactcgat gaaaacgaaa
     9601 atgtcatcgg ttggatcggc ggcatcgaac aatacgacgg gcacgtctgg gaattgcatc
     9661 cgctggttgt gaagcctggg gtgcagggca aaggcattgg ccgcgcattg gttcaggatt
     9721 ttgaagaaca ggttcgaatg cgcggcgcac tcaccgttat gctgggcaca gatgacgaag
     9781 ataacaggac ttccctgtcg aacacggatc tctacgagga accctggaaa aagatcaacg
     9841 agatcaaaaa cctgaaaaga cacccctttg agttttacca aaaaatgggg tatcgtatca
     9901 caggtgttgt tccagatgcc aacggcactg gcaagcctga tattttgatg tctaaaaaaa
     9961 tacaaacgaa acaggatcaa taaaaaatgg aaaaactttt tggcggcatc gagggcggcg
    10021 gaacgaaatt caactgtgct gtgggcaatg gccccgaaaa cattcttgct gaagcgcgct
    10081 ttgcaaccac cacaccagcc gaaacgatca ggcaggtgtg tgattttttc atgtcccatg
    10141 tagatcaact tcaggcgatc ggcttcggtt cgttcggccc atttgatacg gaccccgctt
    10201 cacccaccta cggatacatc accaccacac cgaaaccaca ttggggcaac accaatgtgc
    10261 ttggcatgtt gcgcgagaag atcaacctgc ccttcgcagt ggatatggat gtagctgtgg
    10321 cagggttggg cgaggcaacc tggggcgcca gcaaaaatga tacctgctct ctttacctca
    10381 cggtgggaac agggatcggc ggcggctaca tcatgaacgg caaacccctg cgcggactca
    10441 ccagcctcga aatgggccac atccgcctcg cccgtgaccc gaaactggat ccgtttcaag
    10501 gggcatgtcc gtaccacgga gattgcttcg aaggtctcgc tgcaggaccc gcagtcgaaa
    10561 aacgcttcgg gcaaaaaggt gaaaccctgc cagacgatca tcccttctgg gagttggaag
    10621 caggttatat cgcacaggcc ttggtcaatt acatcctctg cctctcgccg caacggatca
    10681 tcatcggtgg cggcgtgatg caaaaggact tcatgttccc cgctgtgcgc agaaagacac
    10741 aggaactatt gggcggctat atttctcacg atgtgattct caaccacatt gacgaataca
    10801 ttgtcccacc tgcgctgggc ggacgttcgg gagtattggg gagcatcgcc cttgcaatga
    10861 atttgaacgc atgaaaaaga ttccaaacct ccccattgtc gatctcatgc ctttctccga
    10921 gattgatgat aaacgtcccg cattgctcgt cacttctgcg cctgcatgga acgctgtcaa
    10981 aaacaatttg cgcgggctga acatcaccca cacaattgaa gtcaccgaag caaccaccga
    11041 atattgggac aatctccaat taccaattac caatcaccaa attgaaatca tctacgccgt
    11101 gggtggcgga cttgttgctg acgctgccaa atacttcgcc tccaaactca acctgccgct
    11161 tgtggttctt ccaaccgccc tctcggtgga tgcgttcatt accgccgcat caggcatccg
    11221 caaagatgga tgcgtgta
//
LOCUS       DAOQJC010000125        11212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2944_length_11212_cov_9.964686, whole genome shotgun sequence.
ACCESSION   DAOQJC010000125 DAOQJC010000000
VERSION     DAOQJC010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11212
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2944_length_11212_cov_9.964686"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1029
                     /locus_tag="PLE39_15560"
     CDS             130..1029
                     /locus_tag="PLE39_15560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE69798.1"
                     /translation="MIEITGLTKTYGTGNKTVNALNGVDLNINSGMFGLLGPNGAGKT
                     TLMRILAGIVNPSSGSIRINGHDLTTDAGKYAVKSMLGYLPQELGMYTELTAEQFVDY
                     MAILKGLDDSRKRNQRVAEVLEMVGLREVAKRKIKGFSGGMKRRVGIAQALVNDPKIL
                     IVDEPTAGLDPEERIRFRNLLVNLAADRVVLLSTHIVEDIGQTCRDLAVLAKGRILFR
                     GSPAEMLKATEGHVWTLTMSGLEKPNHDLTVVSMLHLAEGTQYRLVGANVNGYSNAQS
                     TQPGLEDGYVWLMKNSDNLVSVE"
     gene            complement(1231..1671)
                     /locus_tag="PLE39_15565"
     CDS             complement(1231..1671)
                     /locus_tag="PLE39_15565"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNE69799.1"
                     /translation="MQFTKDELAALLNGEKPLQLTGLDLSKANLIRAELSKANLSGAD
                     LNGAFLDEARLRYANLNEANLEGAHMNRANFHGANLADADLSDSDLSEADLSGANLAD
                     ATLMYANLTSANLSGANLNGAKYNHHTQWPAGVDPKAAGAVLAE"
     gene            complement(1705..3522)
                     /locus_tag="PLE39_15570"
     CDS             complement(1705..3522)
                     /locus_tag="PLE39_15570"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE69800.1"
                     /translation="MALISLQEVSIGFGGPPLLDKVNLQIEQGEWIGLLGRNGMGKST
                     LLKLVNDDIAPQSGIIARQQDLRTAYLPQEVPAGITGKVFDIVAGGLDSLKPVPASDE
                     HHWRGQLQVEQVISRMQLDGEARFEILSAGMKRRVMLARGLVRQPELLLLDEPTNHLD
                     IASIDWLETFLKRWGGTLLFVTHDRVFLQKLTSRIVELDRGRLFDWNCDYSTFLERRD
                     AMLSAEAEQNALFDKKLAQEEVWIRKGIEARRTRNEGRVRALKRLREQRKERRERPGK
                     VKMQIQSEARSGKIVIEAENVSYAYDGRTIVKEFTTTIQRGDKIGIIGPNGSGKTTLL
                     RLLLEELTPQTGTVEHGTNIEIGYFDQLRAQLDETKSVLDNVGQGSDNVTINGRSRNL
                     NVYLEDFLFSRDRINAPISALSGGERNRLMLARLFAQPANLLVMDEPTNDLDVETLEI
                     LEELLLDYEGTLLLVSHDRAFLNNIVTSTLALDGSGRVIETVGGYDDWAKQNESAPIE
                     SKPRKAAVGLENEARSSSSKKPSYKEQRAIEAQKRELAELPQRIEKLEADLHALTAEM
                     ATPNFYQRDSAEIAQTAERLKQMQDELNQAYARWEELET"
     gene            3649..4800
                     /locus_tag="PLE39_15575"
     CDS             3649..4800
                     /locus_tag="PLE39_15575"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNE69801.1"
                     /translation="MASEERFNELDGMRGIATILVISYHIFKRGDYFTTNAVLHFITS
                     LTLYGWYALDTFFVLSGFLITGILLRTKEEKDYFKNFYVRRSLRVFPLYYFVLALILF
                     LMPKLDPDYVAEIPKALPYYLFYQQNWFHFLTNVKGTEHLSVTWSLAIEEQFYLLFPF
                     LVYFTRKETLAKVAGGIIFVSILARVLSAFLWTDVDQMTNFYFYNTFTRFEEISFGIL
                     IAVAFTYPDWKAKLSRIAMPVFLIAYPALLITEAVTSRSIGIPHPAYGNVPLTVVGYT
                     LASVFAAALIVVLTTHDRMSFIRRFFRNKVLTFFGDHSYSIYLFHMPIGLTLLDYLWR
                     NDYRGWQIYFLYIGLTFGITIFISILTWNFLEKPMLGLKKYFEYKEPAK"
     gene            complement(4909..5571)
                     /locus_tag="PLE39_15580"
     CDS             complement(4909..5571)
                     /locus_tag="PLE39_15580"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNE69802.1"
                     /translation="MSLDASRIKALCFDVDGTLSDTDDLYAQKVERLFPRFLFKNPAR
                     AARRFVMWVEAPGNALLGYADRLGLDDEMVAIINWISRHRKHSDKKFLLVPGVDDMLK
                     QLHGRYPMAVVSARDERGTLAFLEQFGLVKYFDAIVTGLSAEHTKPYPDPVLLAARKM
                     NVPAENCLMIGDTTVDIRAGKSAGAQTVGVLCGFGEEPELKKLGADLILSDTPKLLDV
                     LK"
     gene            5654..7786
                     /locus_tag="PLE39_15585"
     CDS             5654..7786
                     /locus_tag="PLE39_15585"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE69803.1"
                     /translation="MTEDRSRSLLELLVNISREVATALDLRTVLQRLLFAALQYVGGE
                     RGSIVVMDDLGKPVDATIVYGKQLHEHTTQQLRDTVERGLAGWVIQRRKAALVPDTSK
                     DERWLRREDDSVDRSGAKSALCVPLMARERLVGVLTLVHSIPNAFTEEHLDLMRAIAD
                     QASVAVLNARLYTESQRTARVMSSLAEGAAAINTSLELPDVWRRILNQTMQALQVETV
                     ALALIDNTTDDIVFQAAAGHNSGNIPGRHIKNGRGLTGLVVSNGFGLIVPNVNADERF
                     SEVDKFGGIVMRALAIVPIQTQGKVIGILEAINPISGAFDMDALLVLTGLGSLAGTTI
                     QNAQFMERLKRAHERYRELFEDSVDPILITDWEGRVLEANRQAVSLSGYTNEQLHNMS
                     IDQLHEVKWNNTGLNFETLKHNDECNYESDLHQQNGGVVPIEVHAHRVEIEESDSVQW
                     ILRDITERKELDALRKDMTDMIYHDIRSPLGNIVSSLEMMKDMLPENDETLFTMLNIA
                     RSSAGRIQRLVYSLLDTNRLESGQPIVDQHAIDPVALIRESVRDTERGVENREQTLEN
                     KANTVLPLIWVDVDMIYRVFINLIENAIKYTPMGGHIEVGAQTTDGMFVKFWVKDNGP
                     GIPPQDRDRVFDKFTRLRGKNNNPSGIGIGLSFCKLAVQGHGGKIWVESEVDKGTTFF
                     LTLPVAQKKATGQLKRQTGRLTIKEDKL"
     gene            7811..8668
                     /locus_tag="PLE39_15590"
     CDS             7811..8668
                     /locus_tag="PLE39_15590"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE69804.1"
                     /translation="MQEITNNVFIEDQFPGVTLGVIVTPRGLIQIDAPPSPEDARSWR
                     AALMNLGGGMERVLVNLDAHPDRTLGARAMDCTVIAHEKTANTFRTRPNTFKAQGEET
                     GADWESIAGLGSVRWAPPEISFLNQMTLHWSDSPVFLEHHPGPASGSIWVHLPNEKIL
                     FVGDAVLKNQPPFFAGSNLANWLTTLNLLLSPAYKGYTIISSRGGVVTANIVKAQLDF
                     IKHTHDKLEKSTTKKPNPAATEKLVTSLLTWFKAPAARQKQFAQRLRYGLLHYNARPN
                     HTAGQYNED"
     gene            8671..9723
                     /locus_tag="PLE39_15595"
     CDS             8671..9723
                     /locus_tag="PLE39_15595"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNE69805.1"
                     /translation="MRQPLLELTRGRVVESTHFGSIAIVDSNGKLLHSYGDPYEVAFL
                     RSSAKPFQALPFIERGGAEHFHYTQSEISISCASHETAQIHLDTVRAMQAKIGIQETD
                     LQCGGHLPGDVPVLKQVILQGILPTANFNNCSGKHTSMLAHAKMRGLPLENYLDRSHP
                     IQQDILTTVSEMCKFDRDKIELGIDGCSAPNFALPLFNAALGMARMCDPRELSEPRAA
                     ACQKITAAMIAHPEMVSNFGEFDCELMQSCQGRVITKRGAEGFQLIGLMPGVYGEYGV
                     GIAFKVTDGDASRMNDELESSVRVRPAVTLEILRQLKAIQDAQLKSLQAFGPKKQLKN
                     HRGLVTGESYPAFQLA"
     gene            9768..10493
                     /locus_tag="PLE39_15600"
     CDS             9768..10493
                     /locus_tag="PLE39_15600"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNE69806.1"
                     /translation="MQPGVLGLAVVGSYARGEHTAHSDIDLDIFVESLSGEADYTLTH
                     MEGRLVSLKYLTIAQELESLGKPQKAIWAVPGLRQMVILTDDTGAIANLKQAAQNFNW
                     QAMQPAADEYAAEELMSCAEEIHKIVHGLSADNESKVLYAVWGLFRGLANAVAVQQGM
                     MMTSENRYFDIIQQTIGATHPWTKSFRLALGADIIATQPAYITRGKAALDLYRHTAVL
                     FQNIIPEKHRDVIENTLQLLDTL"
     gene            10490..11155
                     /gene="nth"
                     /locus_tag="PLE39_15605"
     CDS             10490..11155
                     /gene="nth"
                     /locus_tag="PLE39_15605"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNE69807.1"
                     /translation="MKNPKERALLIHQTLLTAFGEPIWRNPLPAIDELVSTILSQNTN
                     DINRDRAFDALRAKFPTWESVRDANAEDVIAAIKPAGLANQKGPRIQQVLRAITEERG
                     SLNLDFLAGLPIEEARSWLTKFNGVGPKTAAIVLCFSLNMPAFPVDTHVYRVTGRIGL
                     RPEKMTVEQAHPHLESVLPPETYYAAHLNIIRLGREICHARKPNCPQCPIVKLCEYKE
                     KTK"
BASE COUNT         2699 a         3142 c         2773 g         2598 t
ORIGIN      
        1 taaccacgga gtcacggaga cactgagaaa atcataaaaa ctccgtgact tcgtgcctca
       61 gtggttcaaa atgtcgcgct tgaaatcgcg agataaatct cgcagtactt tacgaaaaag
      121 gaactcacca tgatcgaaat cactggactc accaaaacct acggcactgg aaacaaaacc
      181 gtcaacgccc tcaacggcgt ggacttgaat atcaactctg gcatgttcgg cttgctcggt
      241 cccaacggcg caggcaagac caccctcatg cgcatcctgg cgggcattgt caaccccagc
      301 agtggatcca tccgtatcaa cggacatgac ctcaccaccg atgcgggcaa atatgcggtc
      361 aagtccatgc ttggctatct tccgcaggaa cttggcatgt acaccgaact gaccgccgaa
      421 caattcgtgg attacatggc catcctcaaa ggcttggacg actcccgcaa acgcaatcaa
      481 cgcgtggcag aagtgctgga gatggtcggt ctgcgcgaag tggcgaagcg caaaataaaa
      541 ggcttctctg gcgggatgaa acgccgcgtc ggaatcgctc aagccttggt caacgacccg
      601 aagattctca tcgtggatga gccaacagcg gggcttgacc ccgaagagcg gattcgcttc
      661 cgcaatttac tggtcaacct cgccgcagat cgtgtcgtcc tgctttccac acacatcgtc
      721 gaagacatcg gtcagacctg ccgcgatctg gctgtgcttg caaaaggaag aattctcttt
      781 cgcggcagtc ccgctgaaat gctcaaagcc accgaaggcc acgtctggac attgactatg
      841 tctggtttgg aaaaaccgaa ccacgacctg actgtcgtct ccatgctgca tcttgcggaa
      901 gggactcagt atcgactggt cggtgcaaat gtcaacgggt attcgaatgc acagtccaca
      961 caacctggtt tggaagatgg ctatgtgtgg ttgatgaaaa actctgataa tctggtcagt
     1021 gtggaatagc ggacaaaaca aaaatattta caaatcacga atcacatcgg gataaatccc
     1081 taaaaaggat ttcagaaccc attgtgaaag ttttatcaga agccagtatg ttctgtgtat
     1141 ttcacaaaca gatcccccac tttgccttaa gacgaacgaa cctcgaaact ttactttcga
     1201 ggttcgttct tttgtcctgc aagtttattt ttattcagca agcactgcac cagccgcttt
     1261 tgggtcaacc ccggcaggcc attgcgtgtg gtgattgtat ttcgcgccgt tcaaatttgc
     1321 accgctcagg ttggccgagg tcaggttggc atacatcaaa gttgcatcgg ccagattcgc
     1381 cccgctcagg tcagcctcac tcaaatcact gtcgctgaga tcggcatcag ccaaattggc
     1441 cccgtgaaaa ttcgcccgat tcatatgggc gccttccaaa ttggcttcgt tcaggttggc
     1501 atagcgcaat ctggcttcat ccaaaaaggc tccgttcaag tcggcgccgc tcaggttggc
     1561 cttgctcaac tcagccctga tcagattggc cttgctcaag tcaaggcctg tcaattgcag
     1621 cggcttctct ccattcaaca atgcggcaag ttcatctttt gtgaattgca tggtatcgcc
     1681 tttccataat ttacttcaat actactacgt ttccaattct tcccaacgcg catacgcctg
     1741 gttgagctca tcctgcattt gcttcagccg ctctgctgtc tgcgcgatct cggcactatc
     1801 ccgctgataa aaattcgggg tcgccatctc agccgtcagt gcgtgaagat ccgcttccag
     1861 tttttcgatc cgctgtggaa gttcggcaag ttcccgtttt tgagcttcga tcgcacgctg
     1921 ttccttgtaa ctcggttttt tggaggagga agagcgcgcc tcattctcca agccgacagc
     1981 tgccttgcgc ggcttcgact cgatcggcgc cgattcattt tgcttcgccc aatcgtcgta
     2041 tccgccgact gtttcgatca cccgtccgct gccatcaagg gccagtgtgc tggtgacaat
     2101 gttattcaga aaagcacggt catgactgac gagtaacagc gtgccttcat aatcgagcaa
     2161 aagttcttcg aggatctcca atgtttccac gtccagatcg ttggtgggct catccatcac
     2221 aagcaggttg gcaggctggg caaatagacg cgccagcatc aaacgattcc gctcgccgcc
     2281 ggagagcgcg ctgatcggcg cgtttatgcg atcacgtgaa aagagaaagt cctccagata
     2341 cacattcagg ttgcgcgaac ggccattgat ggtgacattg tcactgccct gccccacatt
     2401 atcaagcact gacttggtct catccaattg ggcgcggagt tggtcgaagt aaccgatctc
     2461 gatattcgtg ccgtgttcca ccgtccccgt ttgcggggtc aattcttcca aaagcaggcg
     2521 cagcaaagtc gtttttccag aaccgttcgg gccgatgatg ccgatcttgt cgccgcgttg
     2581 aatggtggtg gtgaactctt tgacgatggt ccgtccatcg taggcatagc tgacgttttc
     2641 cgcttcgatg acgatcttac ccgaacgggc ttcgctctgg atctgcattt tcactttgcc
     2701 cggtcgttcg cggcgttcct tgcgttgttc gcgcagacgt ttgagcgcac gcacacggcc
     2761 ttcgttgcgt gtacggcggg cttcgatccc tttgcgaatc caaacttctt cctgggcaag
     2821 ttttttgtcg aagagtgcgt tttgctcggc ttctgcagaa agcatggcgt cacggcgttc
     2881 gaggaaggtg gagtaatcgc aattccaatc gaacaggcgt ccacggtcca attcgacaat
     2941 gcgggaggtg agtttttgta aaaagacgcg gtcgtgggtg acgaacaaca aggttccgcc
     3001 ccagcgtttg agaaaggttt caagccagtc aatggaggcg atatcgaggt ggttggtggg
     3061 ttcatcgagc aaaagcagtt cgggctggcg taccaatcca cgggcaagca tgacacggcg
     3121 tttcatccca gcggagagga tctcaaaacg ggcctcgcca tccaactgca tccgcgagat
     3181 gacctgttcc acttgaagct gtccgcgcca gtgatgttcg tcggaggcag ggacaggttt
     3241 cagtgagtcc agaccgcccg caacaatgtc gaataccttt cctgtgatcc cagcagggac
     3301 ttcctgcgga agataggcgg tgcgtaaatc ctgttggcgg gcgatgatgc cgctttgtgg
     3361 ggcaatatca tcgttgacca gtttcagcag ggtggatttt cccatgccgt tgcggcccaa
     3421 taagccgatc cattctccct gctcgatctg tagattaact ttatcaagca gcggcggacc
     3481 gccaaacccg atactgactt cctgtaaact gatcaatgcc atgaaatttt tcctcgtagg
     3541 gggcagaata tactacgaaa taccaaaccc cgcgggttta ctattcagaa aataaacgat
     3601 agtacaatta cgcaccttat tattcctgtg aacgaaaagg atgcttccat ggcgtcagaa
     3661 gaacgtttca atgaactgga tggcatgcgt ggaatcgcaa cgatcctggt catctcctat
     3721 catattttca aacggggcga ttatttcacg acaaatgcgg tactgcactt catcaccagc
     3781 ctgaccctgt atggatggta tgcgctggat actttttttg tcctttcagg ttttctcatc
     3841 actgggatcc tgttgaggac gaaggaagag aaagattatt ttaagaactt ttatgtccgc
     3901 cgttccctgc gggtctttcc tctttattat tttgtcctcg ctttgatcct gtttctgatg
     3961 cccaaactcg accccgatta cgtggctgag attccgaagg cgctgcctta ttatcttttc
     4021 tatcaacaga actggttcca ctttctcacg aacgtgaaag gcacggagca tctctcggtg
     4081 acctggtcac tggccattga agaacagttt tacctgctgt tcccttttct ggtctatttt
     4141 acgcgcaagg aaacactggc aaaggtggcg gggggaatca ttttcgtttc cattctggcg
     4201 cgcgtgcttt ccgccttttt gtggacggat gttgaccaga tgacgaactt ttatttctac
     4261 aacacattca cccgttttga agagatctca tttgggattt tgatcgccgt tgcatttacc
     4321 taccccgatt ggaaggccaa actcagcagg atcgccatgc ctgtcttttt gatcgcctac
     4381 cctgctttgc ttatcacaga agcggtcacc agccgaagta ttggcattcc ccaccccgcc
     4441 tacggcaatg tgccgctgac cgtcgttggg tacacactgg cttccgtttt tgcggcggcg
     4501 ctcattgtgg tgttgaccac ccatgacagg atgagtttta tccgccgatt tttccgcaac
     4561 aaggtgttga cctttttcgg cgaccacagt tattcgatct atctcttcca catgcccatc
     4621 ggcctcaccc tgctcgatta tttgtggcgg aatgactatc ggggctggca gatctatttt
     4681 ctctacatcg gtctgacttt tggaatcacc atcttcatct ccatactgac ctggaatttc
     4741 cttgaaaagc cgatgcttgg cctgaaaaag tattttgaat acaaagagcc cgcaaagtaa
     4801 acagccattg ctgccagagg tttaaaaaca taactcaccc acggaagctg aatttcgggg
     4861 tgagttatgt ttttgatgtt gattttgcct acttcatgac acaacaggtc actttagcac
     4921 atccaacagt ttcggcgtgt ctgaaaggat cagatccgca ccaagttttt tcagttcagg
     4981 ttcctcccca aacccgcaaa gcacaccaac ggtctgcgca cccgcggact tccccgcacg
     5041 gatatccact gtagtatcgc cgatcatcag gcaattttct gcgggaacat tcattttccg
     5101 tgccgccaac aaaacagggt ctggatacgg cttggtatgt tctgcagaca ggcctgttac
     5161 gatggcatcg aaatatttca ccagcccgaa ttgttcgaga aaagccagcg tgccgcgttc
     5221 atcccgtgcg ctgaccacag ccattgggta acgtccatgc agctgcttca acatgtcatc
     5281 cacacctggt acgagcagga attttttatc cgaatgtttg cgatggcggg aaatccaatt
     5341 gataatcgcg accatttcat cgtcaagacc cagcctgtcc gcgtaaccaa gcagggcatt
     5401 gccaggtgct tccacccaca tgacaaagcg ccgcgccgca cgagcagggt ttttgaataa
     5461 aaagcggggg aaaagccgct ccaccttttg cgcatacagg tcatcggtat cgctcagggt
     5521 gccatccacg tcgaaacata aggctttaat acgggaagca tcaagtgaca tgcgcgcaat
     5581 tgtaccaaaa cggctccctc cgtccttcag ggttattcga aaaggtttgc gtagtataat
     5641 ttaacaaata tttatgactg aagatcgctc tcgttcattg cttgaattat tggtcaacat
     5701 cagccgtgag gtcgcaaccg cgttggacct tcgcacggta ctacagcgat tgctttttgc
     5761 ggcccttcaa tatgtgggcg gcgaacgcgg cagcatcgtt gtcatggatg atcttggcaa
     5821 accagtggat gccaccattg tctacggcaa acaactccat gaacatacca cccagcaatt
     5881 gcgtgacaca gtggaacgcg gtcttgcagg ctgggtgatc cagcgccgca aagctgcact
     5941 ggtacctgat accagcaagg atgaacgctg gctgcgtcgt gaagatgact ctgtggatag
     6001 aagcggggca aaatcggcgt tgtgtgtgcc gttgatggca cgtgaacgtc tggtgggtgt
     6061 gttgaccctc gtccactcaa ttcccaatgc ttttactgaa gaacatctgg acctgatgcg
     6121 agccatcgcc gatcaggcca gtgtggccgt tttgaacgcg cgcctctaca ccgaaagcca
     6181 gcggaccgcc cgtgtcatgt cctcactggc agagggggcg gcggcgatca acacttcgct
     6241 ggaattgccc gatgtgtggc gccgcatcct taaccagacc atgcaggcct tacaggtcga
     6301 gaccgtggca ctggctttga tcgacaacac caccgacgac atcgtttttc aagctgcagc
     6361 aggtcacaac tcaggcaata tccccggcag gcacatcaaa aatggccgcg ggttgactgg
     6421 gttggtggtc agcaatgggt ttgggttgat cgtacccaac gtcaacgcag acgaacgctt
     6481 tagtgaagtg gacaaattcg ggggaatcgt gatgcgggcc ttggccatcg tcccgatcca
     6541 gactcaggga aaggtcatcg gcatccttga ggccatcaat cccatctctg gcgcattcga
     6601 catggacgcg ctgctggtgt tgaccggtct cggcagcctt gcaggcacaa ccatccaaaa
     6661 cgcccagttc atggagcgat tgaagagagc ccatgaacgt taccgcgaac tgtttgaaga
     6721 cagtgttgac ccgatcttga tcacagattg ggaaggccgt gtactggaag ccaatcgtca
     6781 ggcggtaagc ctgagtggtt acaccaatga gcagttgcat aacatgagca ttgaccagct
     6841 gcatgaggtc aagtggaaca acaccggtct caactttgaa accctcaagc ataacgatga
     6901 atgcaattac gaatcagacc tgcaccagca gaatggcggg gttgtcccca ttgaagttca
     6961 tgctcatcgt gtggagatcg aagaaagcga ctcggttcag tggatactgc gtgacatcac
     7021 cgaacgcaag gaactggatg ccctgcgcaa agacatgacc gacatgatct atcacgatat
     7081 ccgctcgccg ctcggcaaca tcgtttcaag ccttgagatg atgaaggata tgctgcctga
     7141 gaacgacgag acccttttca ccatgctcaa catcgcacgc agttctgcgg ggcgcattca
     7201 aaggcttgtg tattctttac tcgacacaaa ccgcctcgaa tcaggtcagc cgatcgttga
     7261 tcagcacgcc attgacccgg tggcgctgat tcgcgaatcg gtccgcgaca ccgaacgcgg
     7321 cgtggaaaac cgcgagcaga ctctggaaaa caaggccaac accgtcctcc cgctcatttg
     7381 ggtggatgtg gatatgatct accgcgtatt catcaacctg atcgagaatg ccatcaagta
     7441 cacgcccatg ggcgggcata ttgaagtcgg cgcgcagacc accgatggaa tgttcgtcaa
     7501 gttttgggtg aaagacaatg ggcccggcat tcccccgcag gaccgcgacc gcgtcttcga
     7561 taaattcacc cgtctgcgcg gcaagaataa caaccccagc ggcatcggca ttgggctgtc
     7621 tttctgtaag ctggcagtgc agggacatgg tggaaagatc tgggttgaaa gtgaagttga
     7681 taaaggcacc accttcttcc tcaccctgcc ggttgcccaa aagaaagcca caggccagct
     7741 caaacgtcag acaggccgtt tgaccatcaa ggaagacaag ctttaatttt ccattttttt
     7801 caggagcagc atgcaggaaa ttaccaataa cgttttcatc gaagatcaat ttcccggggt
     7861 tacactcggc gtgatcgtca caccccgcgg attgatccag atcgatgcgc ccccctcccc
     7921 cgaagacgca cgctcatggc gcgcggcttt gatgaatctc ggcggcggca tggaacgggt
     7981 tctcgtcaac ctcgatgcac accccgaccg caccctcggc gcacgtgcca tggattgcac
     8041 agtgatcgcc cacgaaaaaa ccgccaacac ctttcgaacc cgcccgaaca ccttcaaggc
     8101 tcaaggggaa gaaacaggcg cagattggga atccattgcc gggttgggaa gtgtccgttg
     8161 ggcacctcca gaaatttcct ttttaaatca aatgaccctg cattggagcg attcccccgt
     8221 atttttggaa catcaccccg ggccagccag cggttccatt tgggtacacc tccccaatga
     8281 aaaaatattg ttcgtcgggg atgctgtctt gaaaaaccag ccgccttttt ttgcgggttc
     8341 aaaccttgcc aactggctta ccaccctcaa cctgcttctg agcccggcct acaaaggcta
     8401 taccatcatc agcagccgcg gcggtgtggt gacggccaac atcgtaaaag cgcaactcga
     8461 tttcatcaaa cacacacacg acaagctgga aaagagcacg acgaaaaaac cgaatccagc
     8521 ggcgaccgag aaactggtta cgtcactcct gacatggttc aaggccccgg ccgcccgcca
     8581 gaaacaattc gcccaacgcc tgcgctatgg gttgcttcac tacaacgccc gcccaaatca
     8641 caccgccggg caatacaatg aggattaaac atgcgccaac ccctgcttga actgacccgc
     8701 gggcgcgtgg tcgaatcaac ccactttggt tccatcgcca tcgtggattc caacggtaaa
     8761 cttctgcact cctacggcga cccatacgag gtcgcctttt tacgttcatc agccaaaccg
     8821 tttcaggcac tgccattcat tgagcgcggc ggagcggaac atttccacta cacacaaagc
     8881 gagatatcaa tctcatgcgc ttcgcacgaa accgcacaga ttcatctcga tacggtcaga
     8941 gccatgcagg ccaagatcgg cattcaggaa accgacctgc aatgcggcgg acacctgccc
     9001 ggggatgtgc ctgtattgaa gcaggttatc ctgcagggaa tcctgcccac cgctaatttc
     9061 aataactgct ctggcaagca cacctccatg cttgctcacg ccaaaatgcg cggactgcca
     9121 ctggaaaatt atctcgatag aagccatccc atccagcagg acattctgac aaccgtttca
     9181 gagatgtgca aatttgaccg tgacaagatc gagcttggca ttgacggctg ttctgcgccc
     9241 aattttgccc tgcccttgtt caacgcagcc ctcggcatgg cacggatgtg cgatccgcgt
     9301 gaattgagcg aaccccgcgc agcggcctgt caaaagatca cggccgccat gatcgctcac
     9361 cctgaaatgg tgagcaactt cggcgagttc gattgtgaat tgatgcaatc ctgccaggga
     9421 cgggtcatca ccaagcgcgg cgcggaaggc ttccaactca tcggcttgat gcccggtgtg
     9481 tatggcgaat acggcgttgg tattgccttc aaggtcaccg atggcgatgc ctcccgcatg
     9541 aacgacgagc ttgaatccag tgtgcgtgtg cggcctgcag ttacgctgga aatcctgcgt
     9601 caattgaaag ccattcaaga cgcacaactg aaatctctgc aagccttcgg ccccaaaaaa
     9661 caactcaaaa atcaccgcgg actggtcaca ggcgaatcgt accccgcctt ccaactcgca
     9721 tgaacctcca cgaagaccca ttcctcaaat ctctgagcgc ccggctaatg caaccgggcg
     9781 ttcttggtct tgcagtggtt ggcagctatg cacggggcga acacactgcc cacagcgata
     9841 ttgacctgga tatcttcgtc gagtcgcttt cgggtgaagc cgattacacc ctcacccaca
     9901 tggaaggtcg ccttgtcagt ttgaaatatc tcaccatcgc acaggaactg gaatcgcttg
     9961 gcaaaccaca aaaagcgatc tgggctgtac cggggctgag gcaaatggtc atactcaccg
    10021 acgatacggg cgcaatcgca aacctcaaac aagctgcaca aaatttcaat tggcaggcca
    10081 tgcaacccgc cgcagatgaa tacgccgccg aggaattaat gagttgcgct gaggaaatac
    10141 acaagatcgt ccacgggctt tcagcggata acgagtcaaa ggtcttgtat gctgtgtggg
    10201 ggctgttcag aggattggcc aatgcggtcg ccgttcaaca aggaatgatg atgaccagcg
    10261 aaaaccgcta cttcgatatc atccagcaaa ccatcggcgc cactcacccg tggacaaaat
    10321 ccttccgtct cgcgcttggt gcagatatca tcgcaaccca gccagcctac atcacccgtg
    10381 gaaaagccgc cctcgatctt tacaggcaca ctgccgtgct ttttcaaaat atcatccctg
    10441 aaaaacatcg cgacgtaatt gagaatacgc tacaattgct cgacacatta tgaaaaatcc
    10501 aaaagaacgc gcgctcctca tccaccaaac tctgctcacg gcattcggcg agcccatttg
    10561 gcgcaacccg ctccctgcca tagatgagct cgtctccacc atcctctcgc aaaatacaaa
    10621 tgacatcaac cgcgaccgcg ccttcgatgc cctgcgcgca aaattcccca cctgggaatc
    10681 tgtccgcgat gcaaacgcgg aggatgtgat cgccgcgatc aaacccgcag gattggcgaa
    10741 tcaaaaaggc ccgcgcattc aacaggtctt acgcgccatc acagaagaac gcggctcgct
    10801 caatctggac tttttagctg gtctgcccat cgaagaggca cgctcctggc tgacgaagtt
    10861 caacggcgtc ggcccgaaga ctgccgccat cgttttatgt ttttcgctga atatgcccgc
    10921 ttttccagtg gatacccacg tctatcgcgt tacaggccgc atcggtctgc gccccgaaaa
    10981 aatgaccgtg gaacaggccc acccgcatct cgaatctgtg cttccgcctg aaacctatta
    11041 cgccgcgcat ttgaacatca tccgcctcgg gcgcgaaatc tgccacgcgc gcaagccgaa
    11101 ttgtccacag tgtcccatcg tcaaactgtg tgaatataaa gagaagacca aataaacatg
    11161 gaaactcccc cagtcagtat tgctttggaa caacttggtg tgccgcacac aa
//
LOCUS       DAOQJC010000126        11141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_2979_length_11141_cov_10.611041, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000126 DAOQJC010000000
VERSION     DAOQJC010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2979_length_11141_cov_10.611041"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="PLE39_15610"
     CDS             <1..828
                     /locus_tag="PLE39_15610"
                     /inference="COORDINATES: protein motif:HMM:NF025986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNE69808.1"
                     /translation="ARRAQDPLAELVKIDPKSIGVGMYQHDVDQTALTHALDGVVESV
                     VNSVGVDVNTASSALLTHVAGIGPKLANNIVTHRDANGPFKSRTALKKVAGLGPKAFE
                     QAAGFMRIRGGANPLDESAIHPESYAIAESILARAALTVDSSIPDRIAALESLTAKIS
                     HEELAKELNCGVPTLKDILEQLVRPGRDPRSDTPAPILRSDVLKAEDLLTGMQLKGTV
                     RNVVDFGAFIDIGVKQDGLLHRTQIPNGTVLKVGDIIDVEIQKIETERGRISLGWVK"
     gene            879..>1063
                     /locus_tag="PLE39_15615"
     CDS             879..>1063
                     /locus_tag="PLE39_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69809.1"
                     /translation="MQLPEAFTSTIKNVYKKDGERFLAVLPVLIDEVSDRWGLTKVTP
                     VPNLSFNFVAFANRGDD"
     assembly_gap    1064..1163
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1164..1648
                     /locus_tag="PLE39_15620"
     CDS             <1164..1648
                     /locus_tag="PLE39_15620"
                     /inference="COORDINATES: protein motif:HMM:NF016533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNE69810.1"
                     /translation="SDNKFIKLSDWFAGLKKIRPHFDGGTGPFPKKLLERVESFLPEL
                     FADTDVKLMHGDFHHFNILSSERGWLVIDPKGVIGPVGYEVGPLMLNPWSSISDWSRF
                     KVHAERRVSILTERLGWEREKIIKWSTAHAILSAWWSIEDNMEDANTIQCAEIFSQLK
                     "
     gene            complement(1677..2342)
                     /locus_tag="PLE39_15625"
     CDS             complement(1677..2342)
                     /locus_tag="PLE39_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNE69811.1"
                     /translation="MKKFVAIAGNIGVGKSTLVKMLCNEMGWEPFYEPVTENPYLADF
                     YQNMSAWAFHSQVFFLTHRLRSHMGLAQHPSSVIQDRSVYEDAEIFAQNLYDQGNMQD
                     RDYQTYRELYETAVQFLPPPDLVIYLRASVPTLLNRINSRGRAYERTIAPEYLQNLND
                     LYESWIENFTLCPVLAVPADDLDYVAHSGHLRLIIAKVEDKLVGKEEVVFEPEEVARA
                     AED"
     gene            complement(2473..3294)
                     /locus_tag="PLE39_15630"
     CDS             complement(2473..3294)
                     /locus_tag="PLE39_15630"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNE69812.1"
                     /translation="MATKTKKTTPVFDSDEEDIIEEAPALIPAPTIQDSSEETITFKK
                     SHFYSVLVVLAFAVGVLVGYVVWGPGSGRSTATAAAAQAAAQAPAGAEQAPQYRRYDI
                     PTEGFPSLGPEDAPIVIVEFSDFQCPFCRRFHDETYQSLLEAYPGQIRFVYRNLPLTS
                     IHPAAMPSAIASLCAKDQNAYWDYHEKLFSSEQLDEETFIRYAADLGLDTEKFTSCLA
                     SGEHDAEIQEDMSFAIDLGVQSTPTFFVNGLAIVGAQPLANFQQLIDKELAGEIP"
     gene            3431..4258
                     /locus_tag="PLE39_15635"
     CDS             3431..4258
                     /locus_tag="PLE39_15635"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNE69813.1"
                     /translation="MRFEIHHPSFPLSNFIDSLIYFEGMRPQHSLDRFLPDGNTELII
                     ELSDAPQYIHHNDTLEIVQSCRYAWVSGVRTHPITIPSGRESTKLVVTFKKGMARAFY
                     PFPMHELTDSVVMADLVFGRRILDFRERLLFTRSPQEMFQLVELFLLNMAGDKLYEDT
                     KVTSVVYAVTSMFRQPTLLNFKKMGEQIGYSQKHFIELFKGQVGVPPKQYLKIARFQK
                     AIKAIEGNRPVQWSDIAMDSGFYDQAHFINDFKAFSGFTPGEYIKRKVPTLNYVPVA"
     gene            4330..4737
                     /locus_tag="PLE39_15640"
     CDS             4330..4737
                     /locus_tag="PLE39_15640"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HNE69814.1"
                     /translation="MENLYINHAAVFVCAIFNLALGALWYSPALFYKAWLAESKLTEE
                     QLAKQNPAKTYGLAFLFSLLISYNLAFFLGDANTNAAWGAAAGFLAGFGFAALIFAVI
                     ALFEQRSWKYVFVNGGFITVYFTVIGFILGIWR"
     gene            4774..5313
                     /locus_tag="PLE39_15645"
     CDS             4774..5313
                     /locus_tag="PLE39_15645"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HNE69815.1"
                     /translation="MAYQFTSIFHLVSAVSALAFGACVLYTRKGTRLHKQLGYAYLLN
                     MLGLNITALFIYQLTGHFGPFHGAALASLLTLLAGFVPAYLRLPHGRWLEFHYEFMNW
                     SYVGLIAAGVSEVATRLPSAPFWPAVVASMFAVFLFGGIWISRSRGKHLSNARNSAIR
                     SDAPVNGWTALRRRRTACQ"
     gene            5334..5903
                     /locus_tag="PLE39_15650"
     CDS             5334..5903
                     /locus_tag="PLE39_15650"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HNE69816.1"
                     /translation="MSSLDKAVQTQLENIQKKTGKSLAELAAIAQKSGLSKHGELRDM
                     FKEKLGLGHGDANALVHAIFQSDGTRAAEGKNEDAVLDEIYSGAKAGFRPIHEKLIKE
                     MKKFGEFEIVPKKGYVSLRRKKQFAMIGPKTNTRFEVGINAKDFKQNARLLEQPKGSM
                     CNYIVNVNDPREVDAELVAWLRSAFDGAG"
     gene            5905..6378
                     /locus_tag="PLE39_15655"
     CDS             5905..6378
                     /locus_tag="PLE39_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69817.1"
                     /translation="MSQSITHEDFFNNLMALLDETFDNTHGIYLDKDTSLFRTLETVS
                     AEEASIPVGGKCASLAAQVAHVTFYMEVLERYVVHHDNSRVDWGEVWRTVEKVTPEEW
                     TASKNKLQATYQRISNLFRENPVWNDDSIGGSLAIVVHTAYHLGEIRQALCVLKK"
     gene            6380..6739
                     /locus_tag="PLE39_15660"
     CDS             6380..6739
                     /locus_tag="PLE39_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69818.1"
                     /translation="MRWGILILVTLTAGWMLADGLRALTIGDYFTPASGEYAGQLGPW
                     TLFVQALGIEPRSTFMKAIFVVYGIAALIAVAGFALNQPWGRNALIAVALLGLWYLPI
                     GTAANVVVLILLFLKRS"
     gene            6783..7439
                     /locus_tag="PLE39_15665"
     CDS             6783..7439
                     /locus_tag="PLE39_15665"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNE69819.1"
                     /translation="MNQSHIIQKTAEYIKQEFSEDSSGHDWWHIYRVWKNAITICEHE
                     KADPFIVELAALLHDLDDWKFNDTGDETPLRAKAWLDSCGVEIPIADEVCEIIKHISY
                     KGAGVENKMKSLEGFIVQDADRLDAIGAIGIGRAFAYGGYKNRPMYDPESAPQMHATF
                     EQYKNSKSATINHFYEKLLLLKDMMNTPTAKKIAEQRHEVMLGFLDQFMKEWEGRDVF
                     "
     gene            complement(7474..9021)
                     /locus_tag="PLE39_15670"
     CDS             complement(7474..9021)
                     /locus_tag="PLE39_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69820.1"
                     /translation="MNRKLDWPTLVLFAALLFGAIVRFWPALTNGFPLNDGGMFYTMI
                     LDLKENNYQLPEFTEYNNADIPFAYPPFGFYIAALLSDLLPVSALQILLWLPALVNTL
                     SIFFFYKLAGQVLNSRLLASLAVLIYALSSRAFLWQVMGGGITRAFGMLFLILMLWQA
                     VQLFKSEGHPTHLALTILFGAGAILSHPQTALHAVLGGAILFGFYGRNKRGVLSVMAI
                     GLGVVLLTAPWWGTVLLRHGFEPLLSAGQTSQRTLESYLAILDFSGLDDYVFLPAFLL
                     AVVGAVAMFKKHEFLLIAWPLLALLADPRGGEGIALLPLSMLAGTGLEKAANWLNRSK
                     EGSSEDPLSNRKAQALLFVLFTYLILGAMIFDFQLVNTSLKPADLEMIQWVKENINER
                     KTFLLSTGREFSMSDPMQEWFPALTSQYSVTTLQGLEWTLNELFFPWHSELVAFQHCA
                     DLNCVTNWSGKNKINYDYLLVTVPASEDKSNSADSLRNLALSAKASPSYTVVYESDST
                     LIFEFHR"
     gene            complement(9029..9673)
                     /locus_tag="PLE39_15675"
     CDS             complement(9029..9673)
                     /locus_tag="PLE39_15675"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HNE69821.1"
                     /translation="MNASPLFEFALLGFISMFTMINPIGVIPVYTAMTAKLSPQESHR
                     VARKASLTALFILLAFALTGQLIFRFFSISVNSLRVVGGVIFFLMGYEMLQAHLSRTQ
                     FDDETTHEYINDISITPLGIPIICGPGAITTAILLMNESSSPLESGIVIGIIIAIITF
                     TYVLLLGAKQVTHLIGENGNKVMLRLMGLIVMVIAVEFFFSGLTPILREIFKIQ"
     gene            9745..>11141
                     /locus_tag="PLE39_15680"
     CDS             9745..>11141
                     /locus_tag="PLE39_15680"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNE69822.1"
                     /translation="MKNKLLQTILSSLAIGFAVLACGQFGATSVPQSPTGVAIATIVP
                     EETLEAVAGSAGLGDSLYPGFGNGGYDVTHYLLDFTIHDVATSDLTAIVTIEANATQD
                     LSSFNLDFIGFDINSLTVNGAEADYSRNGQELIVSPLKPLSDGDAFTVVVQYSGSPEE
                     MQSVALPVQTGWVTVEGGSFVLSEPDGSANFYPVNDHPLDKAAYTFRVTVPKPFEVAA
                     NGLLIETTDKGDATTFVFDSPSLMASYLATINIDEFETETMESDGGIPIRNYYSAGLS
                     EKVRAPFARQGEMIDYFSELFGPYPFEVYGSLVMDVEFGTALENQTMSIYGIDMIDLE
                     DVEGTELTVAHELAHQWFGDSVSVADWRDIWLNEGFATYAEVLWVEHADGSEARDEYV
                     SDLYSDMELYGDVGGAPGDPPADDLFNASVYEWGGLTLHALRLEVGDEAFFEILRTYH
                     ERYKNSNATTEDFVAV"
BASE COUNT         2763 a         2690 c         2922 g         2666 t
ORIGIN      
        1 gcccgccgcg cacaagaccc gctcgccgaa ctcgtcaaga tcgaccccaa gtccattggc
       61 gtgggcatgt atcagcacga tgtcgatcaa accgcgctca cgcatgccct cgacggcgtt
      121 gtcgaaagcg tggtcaacag cgtcggcgtg gatgtcaaca ccgccagctc cgcgctgctc
      181 acgcatgttg cgggcatcgg ccccaaactc gcaaacaaca tcgtcacaca ccgcgacgcg
      241 aacggcccat tcaaatcccg taccgcattg aagaaggtgg cagggcttgg gccaaaagcc
      301 tttgaacagg cagcaggctt catgcggatt cgaggcggag cgaacccgtt ggatgaatca
      361 gccatccatc cagaatccta cgcgatcgca gaatctattc tcgctcgcgc cgccctgaca
      421 gttgattcgt ccatcccaga cagaatcgcc gccttggaat ccctcaccgc aaaaatctct
      481 cacgaagagt tagccaaaga attaaactgc ggcgttccca cattgaaaga catcctcgaa
      541 caactcgtcc gcccaggccg tgacccacgc tccgatactc ccgcccccat cctgcgctcg
      601 gatgtgctca aagccgaaga cctgctgaca ggtatgcagc tcaaaggcac cgtccgcaac
      661 gtggtggact tcggcgcctt catcgacatc ggcgtcaaac aggatggttt gctgcaccgc
      721 acgcagatcc ccaacggcac cgtcctcaaa gtgggtgaca tcatcgatgt tgaaattcaa
      781 aaaatagaaa ccgagcgcgg acgcattagc cttggctggg tgaaatagtc ttgtagggac
      841 acagcgccgc tgtgtcccta cagaatacga tagataaaat gcagcttccc gaagccttta
      901 cctccaccat aaaaaacgtc tacaaaaagg acggcgagag atttctcgct gtccttcctg
      961 ttttaataga tgaggtctca gaccgttggg gattaacgaa ggtaactcct gttcccaatt
     1021 tatcttttaa ctttgtcgct tttgcaaatc gaggtgatga cgannnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnncaagtga caataaattt attaaattat cagattggtt
     1201 tgctgggttg aagaagatac gtccgcactt tgatggcggg acggggccat tccctaaaaa
     1261 gcttttggag cgagtggaat catttttgcc cgagttattt gccgataccg atgtgaaatt
     1321 aatgcacggc gactttcatc acttcaatat tctttcatcg gaacgtggtt ggctggtcat
     1381 tgatccgaag ggagtgattg ggcctgttgg gtatgaggta ggtccgctta tgctcaaccc
     1441 gtggagcagc atttcggact ggagcaggtt caaggtccac gctgagaggc gggtgagcat
     1501 ccttaccgaa aggttgggct gggagcgcga aaagatcatc aagtggtcaa cggctcacgc
     1561 catcctgtct gcatggtgga gcatcgaaga caatatggaa gatgccaaca cgattcaatg
     1621 cgctgagata ttttcccaac taaaataaaa acctccctcg ggaggttttt tatgatctaa
     1681 tcttctgcgg cgcgtgcaac ttcttcaggc tcgaagacca cttcttcttt tccgaccagt
     1741 ttatcttcca cttttgcgat gatcaaccgc agatgaccgg agtgggctac ataatcaagg
     1801 tcatctgctg gaacagccag cacggggcag agtgtgaaat tttcgatcca tgattcgtag
     1861 aggtcgttga ggttctgcag atattcgggg gcgatggtgc gctcgtaggc gcgtccgcgt
     1921 gagttgatgc ggttaagcag cgtgggcacg gaggcacgca ggtaaatgac aaggtccggc
     1981 ggcggcagga attgcacggc ggtttcgtac aactcgcggt aggtttgata atcgcggtct
     2041 tgcatgttgc cctggtcgta caggttttgc gcgaagattt cagcatcttc atacacgctg
     2101 cggtcttgaa tcacagaact ggggtgttgg gcaagcccca tatgtgatcg cagtcgatgg
     2161 gtgaggaaga atacctgcga atgaaaagcc caggcagaca tgttctggta aaaatctgca
     2221 agatatggat tttcagtgac gggttcgtag aacggttccc agcccatttc gttgcatagc
     2281 atttttacaa gcgtggattt tcccacaccg atgtttccag cgatggctac gaattttttc
     2341 atgttctgct ctcaaataaa aaatctttgg gggtgatagg cacgcgggac atcataccaa
     2401 aaagaaaaag agttcacgga aattttaaga acttccgtga actctttatt tgaaatcgat
     2461 cctggcttgc ggttacggga tctccccggc cagttccttg tcgatcagtt gttggaagtt
     2521 ggcgagcggc tgggcgccga caatggcaag cccgttgacg aaaaacgtcg gtgtggattg
     2581 tacgccaagg tcaatggcaa agctcatatc ctcttgaatt tcggcgtcat gttcgccgct
     2641 tgcaaggcag gaggtgaatt tctcggtatc cagcccaaga tcggcagcgt agcggatgaa
     2701 ggtctcttca tcgagttgtt cgctgctgaa cagtttttcg tgataatccc agtaggcatt
     2761 ctggtctttg gcacagagcg aggcgatcgc ggagggcatg gccgccgggt gtatggaggt
     2821 aagcggcagg ttgcggtaca cgaagcggat ttggccgggg taggcttcca acagggattg
     2881 gtaggtctca tcatggaagc ggcggcagaa tgggcattgg aaatcgctga attcaacgat
     2941 cacaatgggc gcatcctccg ggccaaggct ggggaagcct tcagtgggga tatcgtagcg
     3001 tctgtattgc ggagcctgtt cggcacctgc aggagcttgc gcagcggcct gagcagctgc
     3061 ggctgtagcg gtgcttctac cagagccggg accccaaaca acgtacccta ccaacacgcc
     3121 gaccgcaaat gccagcacaa ccagtaccga gtaaaaatga cttttcttga aagtgatggt
     3181 ctcttcggaa gaatcctgta tggtgggggc gggaatcagg gcaggagctt cttcgatgat
     3241 gtcttcttcg tcagaatcaa aaacaggagt ggttttttta gttttagtag ccatgaggga
     3301 attatagccc ttacaagatt tttgtggaaa aggacttagg tttggctaag atttactctg
     3361 ttgaagttct aaaaacgggc ttgcaataac agaatatttg ttctaaaata ctggtattgg
     3421 aaaattcgtc atgcgttttg aaattcacca cccatcattt ccgttgagta attttatcga
     3481 cagcctgatc tattttgaag gcatgcgtcc acagcattct ttggatagat ttctgcccga
     3541 tggaaacacg gaacttatca ttgagttgtc agatgcaccc cagtatattc atcataatga
     3601 cacgttggaa atcgttcaat cctgtcgtta tgcctgggtc agtggcgtgc ggacccaccc
     3661 aatcacaatt ccttcgggca gggagagcac caaattggta gtgactttca aaaagggaat
     3721 ggcccgtgct ttttatccgt tcccgatgca tgaactgacc gattcggtcg tcatggctga
     3781 tctggttttt ggcaggcgca ttcttgattt tcgtgagaga ttgcttttta ccagatcacc
     3841 gcaggagatg ttccaactgg ttgaattatt tctgctgaac atggccgggg ataaacttta
     3901 cgaagacaca aaggttacca gtgttgtata tgcggtcacc agcatgtttc gccaacccac
     3961 tttgctcaat ttcaaaaaaa tgggggagca gatcgggtat tcccaaaaac atttcataga
     4021 attgttcaaa gggcaggtcg gtgtgccgcc aaaacaatat ctcaagatcg ctcgttttca
     4081 aaaagcgatc aaggcaatag aaggaaatcg gcccgtccaa tggagtgata ttgccatgga
     4141 cagcggtttt tacgatcagg cacactttat caacgacttc aaggcttttt ccgggttcac
     4201 tcccggcgaa tacatcaaaa gaaaggtgcc gactctgaat tacgttccgg tggcttaatg
     4261 taaatttttt ccaatacatt tggattcgat ttctgtaaga ttacctccaa aaaacaaagg
     4321 agaaatctca tggaaaacct gtatattaat cacgccgctg tttttgtttg cgctattttc
     4381 aatctggcat tgggcgccct atggtattcg cccgctctgt tttacaaagc ctggctggct
     4441 gaatcaaaac tgaccgagga acaactagca aagcaaaacc ccgccaaaac ctatggactt
     4501 gcctttctgt tttcgctgtt gatctcgtat aacctggcat ttttccttgg agatgcaaac
     4561 acgaatgcag cctggggagc ggcggcgggt ttccttgcgg gtttcggttt tgccgccctg
     4621 atttttgcag tcatcgccct gttcgagcag cgctcgtgga aatatgtttt cgtcaacggt
     4681 ggctttatca cagtgtattt tacggtcatc ggatttatac tcggtatatg gcgttagaaa
     4741 ataataggat gttttctctt tagaaggaag aaaatggcgt atcaatttac atcaatattt
     4801 catcttgtgt ctgccgtctc agcattggcc tttggtgcgt gtgtactgta tacccgcaaa
     4861 gggacacgcc tgcacaaaca actgggatat gcgtacctct taaacatgct gggactcaac
     4921 atcactgcac ttttcattta tcaactcacg ggtcatttcg gaccttttca tggtgcggca
     4981 ttggcgagtt tactcacctt gctggctgga ttcgtccctg cataccttcg ccttcctcat
     5041 gggcgttggc tggaatttca ctatgaattc atgaactggt cgtacgtggg gttgatcgcg
     5101 gcgggagtat cagaagttgc cacccgtctg ccttccgcac cgttctggcc tgcggtggtc
     5161 gccagtatgt tcgctgtctt tttgttcgga ggaatatgga tttcccgaag ccggggaaaa
     5221 catttgtcca atgcgcggaa ctcggcaatt cgatctgatg cgcctgtgaa tgggtggact
     5281 gccttaaggc ggaggcgcac agcctgtcaa tgattttttt aaggagatga tttatgagta
     5341 gcctcgataa agctgtacag acccaattgg aaaatatcca aaagaagaca ggcaagtcgc
     5401 ttgcagaact tgccgccatt gcccaaaaaa gcggattgag caaacatggc gaactccgcg
     5461 acatgttcaa ggaaaagctt gggctgggtc acggcgatgc caacgcgctt gtccatgcca
     5521 tttttcagtc tgacggcacg cgcgctgcgg aaggcaaaaa cgaagatgcc gtgctcgatg
     5581 aaatttattc aggtgcgaaa gcgggctttc gacccattca cgagaaattg ataaaagaga
     5641 tgaaaaagtt cggcgagttc gagatcgttc ctaaaaaggg atatgtcagc ctgcgccgca
     5701 aaaaacagtt tgccatgatc ggtccaaaga ccaacacccg ttttgaggtg ggcataaacg
     5761 cgaaggactt caagcaaaat gcccgtctgt tggaacaacc aaaaggaagc atgtgcaact
     5821 acatcgttaa tgtcaatgat ccgcgtgagg tggatgcaga acttgtcgcg tggctcaggt
     5881 cggcatttga tggggcagga taaaatgtca caatccatca ctcacgaaga ttttttcaac
     5941 aacctgatgg cactgcttga tgaaaccttc gacaacaccc acggtatcta cctcgataaa
     6001 gacacgtctc tttttcggac ccttgaaact gtctctgcgg aagaggcatc gatccctgtg
     6061 ggcggaaagt gcgcctcgct cgccgcacag gtggcccatg taaccttcta catggaagtg
     6121 cttgagcggt atgtggttca ccacgataac agccgcgtgg attggggtga agtgtggcgc
     6181 accgtggaaa aggtgacgcc tgaagaatgg accgcctcaa aaaataaatt gcaggccacg
     6241 tatcaacgca tttcgaattt gttccgcgaa aaccccgtat ggaacgatga ctcgatcggc
     6301 ggttcgcttg caattgtggt tcacacagcg tatcacctcg gcgagatccg ccaggcattg
     6361 tgtgtattga aaaaataata tgagatgggg catcctcatt ctcgtcacct tgaccgctgg
     6421 ttggatgctt gcagatggcc tgcgcgcatt gacgatcggt gattacttca cacccgccag
     6481 cggcgaatat gcgggtcagc ttggtccgtg gacacttttt gtgcaggcac ttggcatcga
     6541 gccgcgctcc acattcatga aagccatctt cgtcgtttat ggcattgcgg cgctgattgc
     6601 tgtggcgggc tttgcattga atcaaccctg ggggcgcaac gcattgatcg cagttgcgct
     6661 tctcggcttg tggtatctgc ccatcggcac ggcggccaat gttgttgtcc ttattttgtt
     6721 attcctcaaa aggagttgac cccgcaccac gtaatgcctg atttctgtgg caaaatagcc
     6781 gcatgaacca atctcacatc atccaaaaaa cagctgaata catcaaacag gaattctccg
     6841 aggactcttc cggacacgat tggtggcaca tctatcgcgt gtggaagaat gccatcacca
     6901 tctgcgaaca cgaaaaagcg gatccattca tcgtggagct ggctgccctg ctgcacgatc
     6961 tcgatgactg gaaattcaat gatacaggcg atgaaacccc gcttcgcgcc aaagcctggc
     7021 ttgattcttg cggcgtggaa attcccatcg ccgacgaagt ttgcgagatc atcaaacaca
     7081 tttcctacaa gggggcgggt gtggaaaata aaatgaaaag cctcgaaggc ttcatcgtgc
     7141 aggatgccga ccgccttgat gccatcggcg ccatcggtat tggccgagcc tttgcctatg
     7201 gcggctacaa gaacagaccc atgtacgatc ctgagtctgc gccgcaaatg catgccactt
     7261 ttgaacagta caagaacagt aaaagcgcca ccattaatca cttttacgag aaattgcttt
     7321 tgctaaaaga catgatgaat acaccgactg ccaaaaaaat agcagaacag cggcatgagg
     7381 tcatgcttgg ctttctcgac caatttatga aggaatggga aggcagggat gttttctaac
     7441 tggcaatctg gattctgttc cctgaaaata tcgtcatcgg tggaattcaa atatcaatgt
     7501 gctgtccgac tcatatacga ccgtgtagga tggagatgcc tttgctgaaa gcgcaaggtt
     7561 tctcagcgag tcagccgaat tgcttttgtc ttcgctggca gggacagtta ccagcagata
     7621 gtcgtagttg attttgttct ttccgctcca gttggtgacg caatttagat cggcgcaatg
     7681 ctgaaatgcg accagttctg agtgccacgg gaagaaaagc tcgttcaagg tccactcaag
     7741 gccctgcaag gtggtgacgc tgtactggct cgtcaacgca gggaaccatt cctgcatggg
     7801 gtcagacatg gaaaattcgc gccctgtgga aagcaggaag gtcttccgct cgttgatgtt
     7861 ttccttcacc cattggatca tttcaagatc ggcgggcttc aaactggtat tcaccagttg
     7921 aaaatcaaag atcatggcgc cgagaatgag ataagtaaaa aggacaaata acaaggcttg
     7981 agccttgcgg ttcgagagcg gatcttcgct tgacccttct tttgaacggt ttagccagtt
     8041 ggcggccttc tccaacccgg tcccagccag catggacaat ggcaacaggg cgatgccctc
     8101 gccgccgcgt gggtctgcca gcagggccag cagaggccag gcgattagca ggaactcatg
     8161 ttttttgaac atggccacag caccgaccac cgccagcaga aaagcaggca ggaagacata
     8221 atcatccagg ccgctgaaat ccaaaatcgc aagatacgat tcgagtgtgc gctgactcgt
     8281 ttgccccgcc gagagtaacg gttcaaagcc gtggcgcaaa agcaccgttc cccaccaggg
     8341 agcggtgaga agcaccacgc ccaacccaat ggccattaca gaaagcactc cacgtttgtt
     8401 gcgcccgtaa aacccgaaaa gtattgcacc gccgagcact gcatgtaatg cggtttgcgg
     8461 gtgactcagg atcgcgcccg cgccaaacag gatggtcaac gcaagatgag ttgggtgccc
     8521 ctcactcttg aacaattgca ccgcctgcca tagcatcaga attaaaaaca acatgccaaa
     8581 ggcgcgggtg atgcccccgc ccattacctg ccacaaaaag gcacgtgacg aaagcgcata
     8641 gatcaacaca gccagtgacg ccaatagacg tgaattgagg acttgtccgg cgagtttgta
     8701 aaagaagaag atcgagaggg tgttcaccaa agctggcagc cagagcaata tttgcagcgc
     8761 agaaacaggc agcaggtcag aaagaagcgc ggcgatgtaa aatccgaatg gcgggtaggc
     8821 gaaggggata tccgcattgt tgtattctgt gaattcgggc agttggtaat tgttttcctt
     8881 caaatcgagg atcatggtgt agaacatgcc gccatcgtta agcggaaaac catttgtgag
     8941 cgcaggccaa aagcggacga ttgcgccaaa gagcagcgcg gcaaaaagca cgagggtggg
     9001 ccaatccagt ttgcgattca tgtgagcctc actgtatctt gaaaatttcg cggaggatcg
     9061 gggtcagacc gctgaagaaa aattccacgg caatgaccat cacgatcaaa cccatcagcc
     9121 gcagcatcac cttgttgccg ttctcgccga tgagatgtgt gacctgtttt gcgccaagca
     9181 gcaggacgta ggtgaaggtg atgatcgcaa taatgatccc gatcacgatc cctgattcga
     9241 gcggtgaaga ggattcgttc atcaacaaaa tggcggtagt gatcgcgccc ggtccgcaga
     9301 tgatggggat gccaagcggc gtgatggaga tgtcgttgat gtattcgtgg gtggtttcat
     9361 cgtcaaattg tgtgcgtgaa aggtgggctt gcagcatttc gtaccccatc aaaaaaaaga
     9421 tcacgccacc caccacgcgc aggctgttga ccgagatgga gaagaaacgg aagatcagtt
     9481 gccccgtcag ggcgaaggcc agcaggatga ataacgccgt caggctggct ttgcgcgcca
     9541 cacgatgcga ttcttgcggc gataattttg cggtcatcgc cgtgtaaacg ggaatgacgc
     9601 caatggggtt gatcatggtg aacatggaaa taaagccaag caatgcaaat tcaaaaagcg
     9661 gggaagcgtt catttgactc caatctgtgc attcaatttt acaacacacc accgtgatat
     9721 actgcctcaa ccacaaggat aaaaatgaaa aataaattac ttcaaacaat tctctcatct
     9781 cttgcgatcg gttttgccgt tcttgcatgc ggccagtttg gcgccacctc cgttccacag
     9841 tcaccgaccg gtgtggcaat tgccaccatc gtccctgaag agacgctgga agcggttgcc
     9901 ggttcggcag gcctgggcga ttcgctctac ccggggtttg ggaacggtgg ctatgatgtg
     9961 acgcattacc tgttggattt tacgatccat gatgttgcca ccagcgacct gaccgcgatc
    10021 gtcaccattg aggcgaatgc cacccaggac ctcagcagtt tcaaccttga ttttatcggc
    10081 tttgatatca acagccttac ggtcaacggg gctgaagcag actactcccg caatgggcag
    10141 gaactgatcg tcagcccgct caaaccgctt tcggatggtg atgcatttac cgtggtcgtt
    10201 caatacagtg gttcaccgga agagatgcaa tctgttgcat tgcctgtgca aacgggttgg
    10261 gttaccgtgg agggcggcag ttttgtgttg agtgaacccg atggctcggc aaatttttac
    10321 ccagtcaatg accatccatt ggataaggct gcctatactt tccgtgtcac ggttcccaag
    10381 ccatttgaag tggccgccaa tggcctgttg atcgagacca ccgataaagg tgacgcaacc
    10441 acatttgttt tcgattcgcc tagtttgatg gcgagttatc tggccacgat caatattgac
    10501 gaatttgaga ccgagacgat ggaatcggat ggcgggattc cgatccgcaa ttattactcg
    10561 gcggggttga gtgaaaaggt ccgcgcaccg tttgcccggc agggtgagat gatcgattat
    10621 ttcagcgaat tatttggccc gtatcccttt gaggtatatg gctcccttgt tatggatgtt
    10681 gaatttggga cggcactcga aaaccagacc atgtccatct atggcattga tatgatcgac
    10741 ctcgaagatg tggaaggcac cgagttaact gtggcacatg aactggcgca ccagtggttc
    10801 ggcgacagtg tcagtgtggc tgattggcgc gatatctggc tcaacgaagg gtttgccacc
    10861 tacgccgaag tcttatgggt ggaacacgcc gatggctccg aggcgcggga tgagtatgta
    10921 agtgaccttt attcagacat ggaattgtat ggcgatgtgg gcggcgcgcc gggcgatcct
    10981 ccagcagatg acctgttcaa tgccagtgtt tacgagtggg gcggactcac attgcatgcc
    11041 ctacgccttg aagtgggcga tgaagccttc ttcgaaattc tgagaaccta tcacgagcgc
    11101 tacaagaaca gcaacgccac caccgaagat tttgtcgctg t
//
LOCUS       DAOQJC010000127        11006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_3038_length_11006_cov_9.247101, whole genome shotgun sequence.
ACCESSION   DAOQJC010000127 DAOQJC010000000
VERSION     DAOQJC010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11006
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3038_length_11006_cov_9.247101"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PLE39_15685"
     CDS             <1..702
                     /locus_tag="PLE39_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HNE69823.1"
                     /translation="PKPGLTWQWQIGNDDIDTSIEADVYDIDLYVDQAIIDELHAKGR
                     KVICYISVGSWEDWRPDKDQFPAEVLGKDYDGWKGERWLDIRQIDKLAPIMLARLDLC
                     QAKGFDALEPDNMEIYTNDTGFPLTYDDQLKFALWLADEAHKRGLAIGQKNASDQVKE
                     LVNIYDFAITEDYFYYDEAEKMLPYIEAGKPVFAAEYTDLPGDFNAFCEKSKQLNFST
                     ILKQRGLDAWIQTCP"
     gene            791..1741
                     /locus_tag="PLE39_15690"
     CDS             791..1741
                     /locus_tag="PLE39_15690"
                     /inference="COORDINATES: protein motif:HMM:NF009498.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HNE69824.1"
                     /translation="MKPITIGKLRGLQQIASKRGTFTALALDHRQNLRKANPAFVDDS
                     ELSRFKLDVTKALAHRATSVLLDPEVSAAQAIAQNVIPSTVGLVVAVESTGYTGDATA
                     RHAQIIPGWSVEKAKRMGASAIKLLVYYHPDSPTAQETEAFTKNIADECARHDLVLML
                     EPLSYSLNDKKLSSDEKRYVVVESAKRLTPLGADILKAEFPLDVAETDQSLWKEACEE
                     ISAATDIPWILLSAAVEYETFLQQVVVACNAGASGIAVGRAVWKEAVTMTSDERVKFL
                     RMVGRQRISRLTSLCHALAKPFYDFYQAEAPFGWHKTYGE"
     gene            1743..2894
                     /locus_tag="PLE39_15695"
     CDS             1743..2894
                     /locus_tag="PLE39_15695"
                     /inference="COORDINATES: protein motif:HMM:NF013473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major facilitator superfamily domain-containing
                     protein 6"
                     /protein_id="HNE69825.1"
                     /translation="MKKIWPFGYYFAFFAGMSAFVPYIVLYYQTLDFSGTQIGLLVAL
                     SSLISLVGGPFMTGLADSGNRHKLIMSLALTVAAVSMFVLPFLRTFLPVLILIGVFSF
                     ISAPANSLADSAAMSMLGDEKHLYGRVRMGGTFGWALTAPLAGIIVARYGLSWAFWAY
                     ALFMVIALLISLNFLYTEQTEANPFWQGVRALLGNRQLMLFMFMAFVCGTAFATVNNY
                     LFAYMNQLHIDESQMGYTLTIATAAEIPVLFFANRLLSQFKPRGLLILSMAATDLRLL
                     LYAFFDSLTGILVFQLLNGLTFPALWVAGVSYSNDNAPPGLSATTQAVFGAMIFGFGA
                     ATGGFLGGILLENLGGQMMYAIFGAVTLVMLVIYVFLEPRFAARHEKAN"
     gene            2878..3795
                     /locus_tag="PLE39_15700"
     CDS             2878..3795
                     /locus_tag="PLE39_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNE69826.1"
                     /translation="MKKPIDILVAGEINPDLILTGDVNPEFDQVEKLVTSATLTVGSS
                     SVIFACGAARLGLRVAFIGVCGDDVFGRFMLAEMQKRNVNVDHVIIRTAGQTGLSVIL
                     NREADRAILTHPGLIADLQASDIADSLLAQTRHLHVASYFLQTKLQPDLPALFQRAHA
                     LGLSTSLDTNYDPSEKWMGFDDLLAITNVFLPNEAEAKSLTGAENVEEAANRLGSKVE
                     ALALKLGKDGALGISKSQRVRVESIPAKVVDTVGAGDSFDAGFIYGYLNGWELKKSLQ
                     LACVCGALSTQRAGGTEGQPTLQEALAFL"
     gene            3792..4718
                     /locus_tag="PLE39_15705"
     CDS             3792..4718
                     /locus_tag="PLE39_15705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HNE69827.1"
                     /translation="MILCLTPNPAIDRSMDVDALQIGEVHRAKKTLAAAGGKGINVAR
                     AIRTLGGEPLCMGPIGGHAGNLLADLAKGEGFSAHWTRVQNETRTCIILVQPNQDATV
                     VNEVGQSIRAEECDALIHDVWEQSERVGLVSVSGSLPPGFPLERYQALLAGLVERGKS
                     VWVDTSGPALKTALTVSGIHIKVNAAELSEALGVEISNAEQAAKAMQHLHQRGISQAV
                     ITLGRHGAVFSSEAGVWLAQPPQLKIVSSVGSGDAFLGGLLFALENGASSETALQKAV
                     AAGAANTLEFGGGTFSLLFFEEIFQNTKISLM"
     gene            4907..11005
                     /locus_tag="PLE39_15710"
     CDS             4907..11005
                     /locus_tag="PLE39_15710"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNE69828.1"
                     /translation="MRDHQQNSFEQWRALLIMGAIILAVGGFLFVFQRLQGPTPDSVP
                     VEVDVTESSPGGDDVQDDNYSILVDLSSGQSQPENTNPLPQATGEPLSSAEIEAILAR
                     LPALTADSAAVDFNIPQEILPPPRPGNTVEETFPPFETESGPGEVEAGPLQVLRFSPE
                     GEIPIAPFISVTFNQAMIPLGTLSDLAATDVPVTIEPPLPGIWRWLGTKTLTFEYDSD
                     EIDRLPKATEFHVTIPAGTLSINGNALAEAVTWTFKTPPPTVVSFYPEDAPQPLDPVF
                     FLQFDQRIDPAAVLKTIAVTAGDQQIEIVPATQEQIEADERVSALTKDAPEGRWLAFR
                     ATQPFKTETRISVVVGPQVPSLEGPLTTTTTQTFGFSTYSPLRVTGQRCSWYEDRCPP
                     LSPFFITFNNPLDLDSFSEDMLSVSPEIPGVSASVYATDLQISGETRGQTTYTVTVSG
                     KIRDIFGQALGEDQQLKFKVDKADPILVNPEQTLVTLDPSSKEPLYSAYVMNYNKLNL
                     KVYAVQPSDWPQYRTYLREWQQDNSNAKMPGRLVEEKTLNLDFPDDTLSEVEIKLRPY
                     MDGEFGHFVLVIEPPAGIFESDDEQWRRFSQTIVIWVQVTQIGFDVYTDHSEMIAWTT
                     NLKDGAPLTGVKIMPENGGPQTLSDAEGIARFDIPNGATYLTASLGADTVLLPRSGYY
                     WDEGGWSAEAPLDTVQWFVFDDRQMYRPGEEVHLKGWLRRVGGRQGGDVELPGDAVRS
                     VSYQLFEPQGNLIGEGKLDVNSLGGFDLAFTLPEGINLGFAQLYLTAEGDVNGLTNLQ
                     YFHSLAVQEFRRPEFEVTARNETAGPYFVGEQATVAVDAKYYAGGGLPGADVRWNVAS
                     NPGTYSPPNWPDFTFGTQRNWWLFSDFLYQNGSFSQGEYFEGQTDALGTHYLNLNFKP
                     QGDPDKNPQPYTITASATVIDVNRQAWSSGTSLLVHPADVYIGLRTASYFVERGEALN
                     VEYIVTDLDGNPVKGREVVITAGVLDWKFKNGDWVEEVVDVQTCSLLSTPEPGLCSFE
                     TPLGGSYQITALVTDELGRKNQSRILRWVSGGKQPPSRNLEQEEVTLIPDKENYQPGD
                     IAKILVQAPFSPAEGLLTVTRNGILYTTRFTVEDGTATLRIPIEEEHIPNLNIQVDLV
                     GSAPRTGDDGETLENIPPRPAFASGNMTLNIPPEQRTLSLQLTPAQSTLEPGGETNLT
                     VLVKDANGKPVADAELAVVVVDEAILALTNYQMKDPLSIFYTTRSSYLSSYYSRASLI
                     LANPQSLAAQSLSRQAADSVLYAAATEAPASVEMPMSEALTLTGGGGETLPDAAIAVR
                     ANFNPLAAFEPTIRTGSNGEARVFIQLPDNLTRYRVMVVAVDDGGSRFGVGESNLTAR
                     LPLMVRPSAPRFLNFGDTFELPVVLQNQTNEPMMVDVVARATNLALENAGQRVTVPAN
                     DRVEVRFNASTLMAGEVQIQFAAVSGEYADAATANLPVYTPATSEAFATYGVLDEGTI
                     AQPVQYPSGVFPQFGGLEVSTSSTALQALTDAVLYLVQYPYECSEQVASRILAVASLR
                     DVLSAFKAEGLPTPSEMEAAVSVDIDRLQSMQNADGGYPYWRRGFESSPFNTIHVTHA
                     LFRAQEKGFNVPVEMQQNSLSYLRDIESHYPSWYGNETRWTLSAYALFVRNLMGDRDS
                     QKAEALLNEAGLENLPMEAIGWLWSVIDDAEQLDAIRLHVNNRVVETPGAANFTTAYT
                     EQTYLLLSSDRRTDAILLDALIEDDPQSDLIPKLVNGLLAAARQTQGRWGSTQENVFV
                     LTALDRYFNTYESQTPDFVARIWLGDTYAGSNEFRGYTTDVSETLIPMNYVLSETSSG
                     GGMQDLILSKDGPGRLYYRLGLQYAPTELDLDPLDMGFVVERRYEGVDDPEDVTLDAD
                     GIWHVKAGARVKVTVTMAADNRRYHVALVDPLPAGFEIINPSLAVSQPVPQDPTSPDF
                     KYGWWWWGPWYEHQNMRDSRAEAFTSLLWDGVYEYVYYVRATTPGTFITPPAKAEEMY
                     SPEVFGRSGTDRVIVK"
BASE COUNT         2473 a         3001 c         2970 g         2562 t
ORIGIN      
        1 cccaaacctg gactcacctg gcaatggcag atcggcaacg acgacatcga cacctccatc
       61 gaagccgatg tctacgacat tgacctgtac gttgaccaag ccatcatcga cgaactccac
      121 gccaaaggac gcaaagtcat ctgctacatc agcgtcggct cgtgggaaga ttggcgaccc
      181 gacaaagacc aattccctgc cgaggtatta ggcaaagact atgatggctg gaaaggtgaa
      241 cgctggctcg acattcgcca gatcgacaaa ctcgcaccga ttatgctcgc ccgtctcgac
      301 ctgtgccaag ccaaaggctt cgatgccctc gaacccgaca acatggaaat ctacaccaac
      361 gacacaggct ttccgctcac ttatgatgac cagttgaaat ttgccctctg gctcgcagat
      421 gaagcccaca aacgcggact cgccatcggg caaaagaatg catccgacca ggtgaaagaa
      481 ctcgtaaaca tctacgactt cgccatcacc gaagattatt tctattacga tgaagccgaa
      541 aagatgcttc cttatattga agcaggcaaa cccgtcttcg ccgccgaata caccgacctg
      601 cctggcgatt tcaacgcctt ttgcgaaaaa tcgaaacaac tcaacttcag caccatcctc
      661 aaacaacgcg gattggatgc ctggatacaa acctgcccgt aaagcagtta caaggttggc
      721 aggttgaaag ttgaaacgtt ccaacctgcc aaccttcaaa cctgaaaact ttaatctgga
      781 accccaaccc atgaaaccta ttaccatcgg caaactccgc ggactgcaac agatcgcctc
      841 caaacgcggc accttcaccg ccctcgccct cgaccatcgt caaaacctgc gcaaagccaa
      901 ccccgccttc gtagacgatt ccgaactctc ccgcttcaaa ctcgatgtga ccaaagctct
      961 cgcgcatcgt gcaacttctg ttttgctaga ccctgaagtc tctgccgcgc aagctatcgc
     1021 gcaaaacgtc attcccagca ccgtcggtct cgttgtcgct gtcgaatcaa cggggtacac
     1081 aggcgatgcc accgcccgcc acgcgcagat catccctggc tggtctgttg aaaaagccaa
     1141 acgcatgggc gcatcggcga tcaaattgct cgtctactat caccccgatt cccccactgc
     1201 ccaagagacc gaagccttta caaaaaacat cgccgatgaa tgcgccaggc acgatctcgt
     1261 gctgatgctc gagccgctat cctattcgct caatgataaa aaactatctt ctgatgaaaa
     1321 aagatatgtt gttgtggaaa gcgccaaacg tctcacaccg ctcggagcag atattctcaa
     1381 agctgaattc ccgctcgatg tcgcagagac agatcaatct ttatggaaag aagcctgtga
     1441 agaaatctct gccgcaaccg acattccctg gatcctgctc tccgctgcgg tcgagtacga
     1501 aaccttcctg cagcaagttg ttgttgcatg taacgcaggc gccagcggaa tcgccgtcgg
     1561 tcgcgccgta tggaaggaag ccgttacgat gaccagtgac gaacgtgtca aattcctgcg
     1621 tatggttggg cgtcagcgca tttcacgcct cacatccttg tgtcatgcac ttgccaaacc
     1681 gttctacgat ttttatcaag ccgaagcgcc gtttggttgg cacaaaacat atggagaata
     1741 gattgaagaa gatctggccg tttggttact actttgcttt tttcgcgggg atgtcggctt
     1801 ttgttccgta cattgttctt tactatcaaa cccttgattt ctcaggcacg cagatcggtt
     1861 tactggtggc gctttcgtcg ctgatctctc tggttggcgg acccttcatg acagggctgg
     1921 ccgattcggg gaaccgtcac aaattgatca tgtctctggc gctgaccgtt gcagcagtat
     1981 ccatgttcgt gctgcctttt ctgcgcacct ttctccccgt gttgatcttg atcggcgttt
     2041 tctcttttat atcggcacca gccaattcac tggccgatag cgccgccatg tcgatgctgg
     2101 gagatgaaaa acatttgtac ggccgtgtgc gcatgggcgg gacatttggc tgggcgctca
     2161 ctgctccgtt ggcggggatc attgttgccc gttacggatt atcgtgggca ttttgggcat
     2221 atgccctttt tatggtgatc gcattattga tcagcctgaa tttcctgtac accgaacaaa
     2281 cagaagcaaa tccattttgg cagggagtgc gtgccttgct gggaaaccgc cagttgatgt
     2341 tgttcatgtt catggctttt gtatgcggta ctgcctttgc aacggtcaat aattatttgt
     2401 tcgcatacat gaaccagctt cacattgacg aatctcaaat gggatataca ctcaccattg
     2461 caacggctgc cgagattcct gtgctgttct ttgccaatcg tttgctgagt caattcaaac
     2521 cgcgcggttt gttgatcctt tcaatggcgg ccacagacct gcgcctgctg ttgtacgcgt
     2581 tcttcgattc gctgacgggc attctggtct tccagcttct caatggactt acgtttcctg
     2641 cgctgtgggt ggcgggggtc tcctattcca acgacaacgc accaccaggg ttaagtgcca
     2701 ccacccaggc tgtattcggc gcaatgatct ttggcttcgg cgcagccaca ggcggattcc
     2761 ttggcggaat ccttttggag aacctcggcg gccagatgat gtacgcgatt ttcggcgcag
     2821 tgacactggt catgcttgtg atctatgtct tcctcgaacc acgctttgcc gctcggcatg
     2881 aaaaagccaa ttgatattct cgttgctggt gagatcaacc ccgacctgat cctgacgggt
     2941 gatgtaaacc ccgaattcga tcaggttgaa aagttggtca cttctgcaac gcttaccgtt
     3001 ggttcgtcct ccgtcatctt tgcttgtggc gcggcacggc ttggattgcg ggttgccttc
     3061 atcggcgtgt gcggcgacga tgtcttcggg cgcttcatgc tcgctgaaat gcaaaaacgg
     3121 aatgtgaatg tcgatcacgt catcatccga accgctgggc agactggtct cagcgtaatt
     3181 ttgaatcgcg aagcggaccg cgcgattctg actcacccag gcctgatcgc tgaccttcaa
     3241 gcatccgata tcgctgacag tttgctggca caaacccgtc acctgcatgt agcgagttac
     3301 ttcctgcaaa ccaaactcca gcctgacctg cccgctttat tccaacgcgc tcatgcactt
     3361 gggctgtcta cttcattgga tacgaattac gacccatccg aaaaatggat gggctttgat
     3421 gacttgttgg caattacaaa tgtcttcctg ccgaatgaag cggaagcaaa gtcgctcaca
     3481 ggcgcagaga atgttgaaga agcagcgaac aggcttgggt caaaggtgga ggcgctggcg
     3541 cttaagctgg gaaaagatgg ggcgttggga atctcaaaga gtcagagagt ccgcgttgaa
     3601 agtattccag caaaggttgt ggataccgtt ggcgcaggcg actcgttcga tgcgggtttt
     3661 atttatgggt atttgaatgg ctgggaattg aagaagtctt tgcaattggc ttgtgtgtgc
     3721 ggagctttat ccacgcaaag ggcgggcggc accgaaggcc aacccaccct gcaagaagcg
     3781 ctggcctttt tatgatcctc tgcctgaccc ccaaccccgc cattgaccgc tccatggatg
     3841 tggatgcatt gcagatcggt gaggtgcatc gcgcaaaaaa gacactggcc gctgcaggtg
     3901 gaaaaggaat caatgttgca cgtgccatcc gcacgttggg cggggaaccg ttgtgcatgg
     3961 ggccgatcgg tgggcatgct gggaatctgc tagccgacct cgcgaagggt gaggggttct
     4021 ccgcgcattg gacaagggtg caaaatgaaa cccgcacttg catcattctc gtacaaccca
     4081 atcaggatgc cacggtcgtc aatgaagtcg ggcaatccat tcgtgcagag gaatgtgatg
     4141 ccctgatcca cgatgtttgg gagcagtcag agcgggttgg gctggtttct gtgagtggca
     4201 gcctgccgcc aggctttccg cttgaacgct accaagcatt gcttgcaggg ttggtggagc
     4261 gcggcaaatc cgtttgggtg gataccagcg gacccgccct gaaaactgca ttgaccgtgt
     4321 cgggaatcca catcaaggtc aatgccgctg aattgagcga ggcgcttggc gtggaaattt
     4381 ccaacgcaga gcaagcggct aaagcgatgc agcaccttca tcagcgggga atttcgcaag
     4441 cggtcatcac gcttggcagg catggcgctg ttttcagttc ggaggcgggg gtgtggctcg
     4501 cgcagccacc tcaattgaaa atcgtcagca gcgttggcag cggcgacgca tttttgggtg
     4561 ggcttttgtt cgcacttgaa aatggcgcat cctctgaaac tgccttgcaa aaagcagttg
     4621 ccgcaggggc ggcgaataca ctcgaatttg gcgggggaac tttttcattg cttttctttg
     4681 aagagatttt tcaaaacaca aaaatttccc tgatgtaaag caggatcatg cccactgcat
     4741 tgaactcgtt ataataacct ctcgaaaagt ctgaaagatt aacctctgcg ggttcttgcc
     4801 ctggcagttc aaaaatgttt ccctgtgatt tttgaaggcg gcaaagtctt tggcgggaac
     4861 cgaacccctg tttgcagcgt cgtgatacgg gaaggaagaa tatgccatga gagatcacca
     4921 gcaaaactcg ttcgaacaat ggcgcgccct gttgatcatg ggtgccatta tcctggctgt
     4981 gggcggtttc ctgtttgtgt ttcaacgttt gcaggggcca acgccagatt ctgtccctgt
     5041 tgaagttgat gtaaccgaat cgtccccggg aggtgatgac gtgcaagacg ataattacag
     5101 cattcttgtg gatttgagca gcggtcaatc gcagcctgag aatacgaatc cattgccgca
     5161 ggcgacgggc gaaccgcttt cctctgcgga gatcgaggcg attctcgcgc gtctccctgc
     5221 gctgacagct gattctgccg cggtggactt taacatcccg caagagattc tgccgccgcc
     5281 gcgcccgggg aacacggtcg aagaaacgtt cccgcccttt gaaactgaat caggcccggg
     5341 tgaggtggaa gcgggtccgc ttcaggtgtt gcgattctca cccgaaggcg agatccccat
     5401 cgcgccattc atcagcgtga cgtttaatca agccatgatt ccgctgggca ccctgagcga
     5461 ccttgccgcc acggatgtgc cagtgacgat cgagccgcct ctgcccggta tatggcgttg
     5521 gcttggtacc aaaacgctga cctttgaata cgactcggat gaaattgacc gactgcccaa
     5581 agccactgaa tttcacgtca ccatccctgc gggcacgctt tcgatcaatg gcaacgcact
     5641 ggccgaagcc gtgacgtgga cattcaagac gccgccgccc accgtggtat cgttttaccc
     5701 ggaggatgcg cctcagccat tggatcctgt tttcttcctt caattcgatc aacggatcga
     5761 ccctgcggct gttttgaaaa ccatcgcagt gacagcgggc gatcagcaaa ttgagatcgt
     5821 gcctgccacg caggaacaga tcgaagccga tgagcgggtg agtgctctga ccaaagatgc
     5881 gcccgaaggc cgctggctgg ctttccgtgc cacgcaacct tttaagaccg aaacgaggat
     5941 ctctgttgtt gtgggtccgc aggttccttc gctcgaaggc ccgctgacca cgaccaccac
     6001 acagacattc ggcttttcca cctactcgcc attgcgtgtg accggacaac gatgttcatg
     6061 gtatgaagat cgctgcccgc cgctctctcc cttctttatc acgttcaaca atccgctcga
     6121 tctggattct tttagtgaag atatgctgag cgtatcacct gagatccccg gcgtttctgc
     6181 cagtgtgtat gccacagatc ttcagatcag cggtgaaaca cgcggacaaa ccacctacac
     6241 ggtaactgtt tctggaaaaa tcagagatat cttcgggcag gcattgggcg aagaccagca
     6301 gttgaaattc aaagtggata aggctgaccc gattctggtg aacccggagc agaccctggt
     6361 tacgctcgac ccatcatcca aagagccgtt gtattcggcc tatgtgatga attacaacaa
     6421 acttaatttg aaggtctatg ccgtacagcc ttcagattgg ccgcaatacc gcacctatct
     6481 gcgtgaatgg cagcaggaca actcaaatgc aaaaatgcca ggcaggctgg tggaagagaa
     6541 gaccctcaac ctcgatttcc ccgatgacac cctctctgag gtggagatca aattgcgccc
     6601 atacatggat ggggagttcg ggcacttcgt ccttgtgatc gaaccgcctg cgggaatctt
     6661 cgaaagcgat gacgagcaat ggcggcggtt ttcgcagacc attgtcatct gggtgcaagt
     6721 gacgcagatc ggttttgatg tgtacaccga tcacagcgaa atgatcgcct ggacgacgaa
     6781 tctcaaagat ggtgcgccgt tgactggtgt gaagattatg cctgagaatg gcggcccgca
     6841 gactttgagc gatgccgaag gcattgcccg ttttgacatt cccaacggcg ctacatatct
     6901 caccgcttca cttggcgccg atacggttct cctgccgcgc agcggttatt actgggacga
     6961 ggggggctgg tcggctgagg ctccgcttga tacggtgcag tggttcgttt ttgatgaccg
     7021 tcagatgtac cgccccggtg aagaggttca cctcaaaggc tggctgcggc gcgtcggcgg
     7081 caggcagggc ggcgatgtgg aattgccagg cgatgcagtg cgttcggtca gttatcagct
     7141 ctttgaaccg caggggaatt tgatcggtga aggcaaattg gatgtgaatt cgctcggcgg
     7201 attcgacctt gctttcaccc tgcccgaagg aatcaatttg gggtttgccc aactctacct
     7261 gaccgccgaa ggagatgtaa acggcctgac caatttgcag tattttcatt cacttgctgt
     7321 tcaggaattc aggcggcccg aattcgaagt cactgcgcgg aatgaaacag caggaccgta
     7381 ctttgtggga gaacaggcca ccgttgccgt ggatgcgaaa tattacgccg gcggcggcct
     7441 gcccggtgct gatgtccgct ggaatgtggc cagcaaccct ggcacatact cgccgcccaa
     7501 ttggcccgat ttcacctttg gcacccaaag gaactggtgg ttattttctg atttcctcta
     7561 tcagaatggc tcgttttcac aaggtgaata ttttgaaggg caaaccgatg ccttgggcac
     7621 gcattatctg aacctgaatt tcaagccgca gggcgacccc gataaaaatc cacagccata
     7681 caccatcacg gccagtgcca ctgtcattga tgtcaaccgt caggcatgga gcagcggcac
     7741 atccctgctt gttcatcctg ccgatgtgta catcgggctg cgtaccgcaa gttattttgt
     7801 cgaacgcggc gaagcgctca acgtggagta cattgtcacc gaccttgatg gcaacccggt
     7861 gaaaggccgc gaagttgtga tcacggccgg ggtgttggat tggaaattca aaaatggaga
     7921 ttgggttgaa gaagtggtgg atgttcaaac ctgctcgctt ctttccacgc ccgagcctgg
     7981 actctgttcc tttgaaactc cccttggggg aagttatcag atcaccgccc tcgttaccga
     8041 tgagcttgga cgcaagaatc aaagccgtat tttgcgttgg gtgagcggcg gtaaacaacc
     8101 gccttcgcgc aacctggaac aggaagaggt cacactcatt cctgacaagg aaaattatca
     8161 acccggcgac attgcgaaga tccttgttca ggctcccttc agccccgccg aaggcctgct
     8221 gaccgtcacc cgcaacggca ttttatacac cacacgcttc accgtggaag atggcaccgc
     8281 cacactgcgc atccccattg aagaggaaca catccccaac ttgaacattc aagtggatct
     8341 ggtcggctct gcgccacgca ccggagatga cggtgaaacc ctcgaaaaca ttccgccgcg
     8401 ccccgctttt gccagcggaa atatgaccct gaacattccg cccgaacagc ggaccctgtc
     8461 tttgcaattg actcccgccc aatccacgct tgaaccgggc ggtgagacca acctgaccgt
     8521 gttggtgaag gatgcaaatg gcaaacccgt agccgatgca gaactagccg tggtggtggt
     8581 ggatgaagcc atccttgcgc tgacaaatta ccaaatgaaa gacccgctca gcattttcta
     8641 cacaacacgg tcatcctatc tttcaagtta ttacagccgt gccagcctca tccttgccaa
     8701 tccacaatca ttggctgcac aatcactgtc aaggcaggcg gctgattccg tgttgtacgc
     8761 tgccgcaacc gaagcacctg catcggttga aatgccgatg tctgaagcgt taacgctgac
     8821 cgggggcggc ggcgaaaccc tgccagatgc ggccattgcc gtgcgtgcca atttcaatcc
     8881 gctggcggct tttgagccga cgatccgcac cgggtcgaat ggagaggcgc gggtgttcat
     8941 tcaactgccc gataacctca cacgatatcg ggtgatggtg gttgcggtgg atgatggcgg
     9001 aagccgtttc ggcgtgggcg aatccaacct tacggcacgt ttgccgttga tggttcgtcc
     9061 ttctgcgccg cgcttcctta attttggcga tacgttcgaa ttgccggttg tgctccagaa
     9121 ccagaccaat gaaccgatga tggtggatgt ggttgcccgc gcaaccaacc ttgcacttga
     9181 aaatgcaggc cagcgtgtca ccgtcccagc caatgacagg gtggaggtgc gtttcaatgc
     9241 ttcgacgctc atggcgggcg aggttcaaat tcagtttgcg gcggtttcag gggagtatgc
     9301 cgatgccgcc acggccaacc tgcccgtgta cacccccgcc accagtgaag ccttcgccac
     9361 ctacggcgtg cttgatgaag gtacgatcgc ccagcctgtg caatacccaa gcggtgtatt
     9421 tccgcaattc ggcgggttgg aggtcagcac atcgtccacg gcgttacagg ctttgaccga
     9481 tgccgtgttg tatctggtgc agtatccgta cgaatgttcc gagcaggtgg catcgcgtat
     9541 tctggcggtg gcttcgctgc gcgatgtgct ttccgcgttc aaggctgagg ggctgcccac
     9601 accgtcagaa atggaagcag ctgtcagcgt ggatattgac cgcttgcagt ccatgcaaaa
     9661 tgccgatggc gggtatccgt attggcggcg cggatttgag tcgagtccgt tcaacaccat
     9721 ccatgtgacc catgccctgt tccgcgcaca ggaaaaagga ttcaatgtgc ctgtggagat
     9781 gcagcagaat tcattgagtt atctgcgcga tatcgaaagc cattaccctt catggtatgg
     9841 caatgaaacg cgctggacgc tcagtgcgta tgcgttgttt gtgcgcaacc tgatgggtga
     9901 ccgcgattcg cagaaggccg aagccctttt gaacgaagct ggcctggaga atcttccgat
     9961 ggaggccatc ggctggttgt ggtctgtgat tgatgatgca gaacaactgg atgcgatccg
    10021 actccatgtg aacaaccgtg tggttgaaac gccaggcgcg gccaatttca ccactgccta
    10081 caccgagcag acctatctcc tgctcagttc cgaccgccgt accgatgcga tcctgctgga
    10141 tgccttgatc gaggatgatc cgcaaagcga tttgatcccg aaactggtca atggcttgct
    10201 cgcggcggcg cgtcagactc agggacgctg gggcagcaca caagaaaatg tctttgtgct
    10261 gacggcgctc gaccgctact ttaacaccta cgaatcgcag acgcctgatt ttgtggcgcg
    10321 catctggctg ggcgatacct acgcgggcag caatgagttc cgcgggtaca ccaccgatgt
    10381 ctctgagacc ttgatcccga tgaattatgt gctgtcagaa acgtcatcgg gcggcgggat
    10441 gcaggatctg attctgagca aggatggccc cggacggttg tattatcgcc tcggcttgca
    10501 atatgcgccc actgaattag acctcgatcc gttggatatg ggttttgtcg ttgaacgccg
    10561 ttatgaaggg gtggatgacc ccgaagatgt gacgttggac gccgatggaa tctggcatgt
    10621 caaagcgggt gcgcgtgtga aggtgaccgt caccatggct gcggataatc gccgctatca
    10681 cgttgcattg gttgacccat tgcctgcagg gtttgagatc atcaatccgt cattggctgt
    10741 ctctcaacct gtgccgcaag acccaaccag ccccgatttc aagtatggct ggtggtggtg
    10801 gggaccgtgg tacgaacatc aaaacatgcg cgatagccgc gccgaagcct tcacctcttt
    10861 actgtgggat ggcgtttatg aatatgtgta ttatgtccgc gccaccacgc cggggacatt
    10921 cattaccccg cctgccaagg ccgaagaaat gtactcgccc gaagtctttg gccgcagcgg
    10981 cacggatcgg gtgatcgtga agtaaa
//
LOCUS       DAOQJC010000128        10919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_3084_length_10919_cov_10.331462, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000128 DAOQJC010000000
VERSION     DAOQJC010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10919
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3084_length_10919_cov_10.331462"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLE39_15715"
     CDS             complement(<1..245)
                     /locus_tag="PLE39_15715"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE69829.1"
                     /translation="MRILLINSEYPPIGGGAGNASANIARCLAALGHEVTVLTSLFGD
                     QPFLETLDGVTICRVRAMRRRQDRSTAFEQLTFIAAAS"
     gene            complement(248..2209)
                     /locus_tag="PLE39_15720"
     CDS             complement(248..2209)
                     /locus_tag="PLE39_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69830.1"
                     /translation="MKPTSSKIFWQTFITATVLMSGLAIYQTVQRVADLEITLWRSKW
                     ILLVGLFALNIFAGMFVLWQLSTARASYLTEKQAAVTKSGINPWAGSVLIAFGFFLVW
                     AVRLFFFGDVLPQIAPIFWVFLWASLIQTFGLQLLTGRDWLTPFVAAVLVQGVVYQIF
                     GHLTIVSAYPFSIGYSEAGRHYYASLFFARPLYGTELALPFLHPTRYFLMSLPFLVDG
                     LPLWAHRLWQALLWIGLTAASSILLIRRLNFTGWVRFALIAWAFLYFFQGAVYYHLQV
                     CVILILAGVSVKHPWRSLLFVVLASVWAGASRVNWFPVPAMLAVAIYLLETPLEGKGW
                     RYWLTPFLWGICGLAAASASQFAYIAVSGNSDARSFGSSFTSDLIWSRLLPNETFPMG
                     ILLGIVIVSAPLIFAFIQMVRGKISSLHPLRWLALIAMLAVLFLGGMVVSVKIGGGAD
                     LHNMDAFLVMLALVVTAFIGGRVAGEREANPAWGAIPVWVVAAAALVPIGFALPQVGF
                     FQRYDSNLVEADLFKLQASTQNVEGEILFVTERQLLIFNDVNNIPLVPEYEQMELMEM
                     AMSGNRDYLENFYADLRSHRFALIVAEEQKFALQKHGAFLEENNAWVRFVGAPILCAY
                     KPVTTLSSTNIQVFAPRENNPECKDPFEK"
     gene            complement(2273..3178)
                     /locus_tag="PLE39_15725"
     CDS             complement(2273..3178)
                     /locus_tag="PLE39_15725"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNE69831.1"
                     /translation="MINWLSKNRQTVARGIGSVLALVLLAVLIQEQGDGELFSAFRRV
                     SLGYFGLALVVLLVSRLFAILRWHILLRSAGVNISFLRSVELTFTGNFSSNFLPTTIG
                     GDVVRLTGAMQLGYDRAICLASLVVDRLVGMAGMALALPFGLVPLFALSNGSTQSVTL
                     SALLQKGVDFIRRTFESLSIWLKKPSALFASLLATLGNQAFIYLMVYFLLLGIGHRLS
                     FWLVAGMYTLSYFVTLIPISINGLGVQELSMTFLLTSLGGLTESESATVAVLVRIIFL
                     ITSLPGAYFLPSILAAMNSKEVDER"
     gene            complement(3171..4133)
                     /locus_tag="PLE39_15730"
     CDS             complement(3171..4133)
                     /locus_tag="PLE39_15730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE69832.1"
                     /translation="MNLSLVVPVYNEQDNLPLLFEAINKVMPTMNRTWEVILVDDGSQ
                     DNSLAVLNEYAKKDFAHVRVISFRRNFGQTAAIAAGLDYAQGEIIVLLDADMQNDPAD
                     IPMMLAKLDEGYDLVSGWRKSRKDNAITRNFPSMIANRIISKVTGVHLHDYGCTLKAY
                     RRDVLEGFRLYGEMHRFIPVYAHSVGAKITEVVVNHHPRKHGKTKYGLERTAKVVLDL
                     FTVKFLISYSSKPIYLFGGAGGFLMVISAIIMLYLFVRRIFFLVAIANSPLLQMSAMF
                     FILGFQSILLGLIAELLVRTYHESQRKPTYTIRTMINLEQDPRD"
     gene            complement(4130..5950)
                     /locus_tag="PLE39_15735"
     CDS             complement(4130..5950)
                     /locus_tag="PLE39_15735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE69833.1"
                     /translation="MLAILGVILYVAQAWRYAHTTIPGLDEGSYLFKGYLYLHGVYEP
                     FEPYGPLTNKAPFAFLIPGFAEYIFGAGLRTGRFFMIFLGLLAILGTWITARRWAGNW
                     FATATVWAYALAPTVIKIHAITASEVIIACMLAWMCVFLLAEERPLWQIVAGSVLGAL
                     AVLTRQNMVVVLPLLILYVFWQHGKQKGLVSFGTASAIFLAVHIYYWPNILTIWAPWL
                     PDGLTPFLNPFRLPKEAVPVWDPSIDFWNRTISFFQGIRYHFIPAAGSIFALMILRIP
                     RDWKTVPAMRAALFLALTYFILFAMHGWAAVASQYESYSCVYCFTPYLSFFDPLGILF
                     TVIVLSHLANRTPSRWILFLGAATLLTLATGFGFSSFDSLSQFFLKFPVPRMSGGQFL
                     PGTTTLLAFITNKFGTELIWVKRYVSAALGLLVGVAVLLIAGIITRRMKDAQTRPGFA
                     FVLLNLSIAMGLILSPLLHVGGRSIDCETDMLVANEQVGAHLASFIPADSLVYWDGGL
                     SFTAMTYVPQARIFPPQINDGYSYRIGGDADTLARFSHWNTELKEEWKASADIFIIQS
                     KRYTLWKDFLNPNEFEEYPKPPVAPSCEDGSELRIFHRLP"
     gene            complement(6149..7111)
                     /gene="gmd"
                     /locus_tag="PLE39_15740"
     CDS             complement(6149..7111)
                     /gene="gmd"
                     /locus_tag="PLE39_15740"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNE69834.1"
                     /translation="MPTALITGITGQDGSYLAEFLLKKGYRVVGVARRSSTVTYERIQ
                     HILDDITVVQGDLHDQGSLLSFLEEYRPTEVYNLAAQSFVPTSWNQPALTGDVTALGV
                     TRLLEAIRFVDPKIRFYQASSSEMFGKVMEVPQRESTPFYPRSPYGVAKMYGHWITVN
                     YRESFNMFATSGILFNHESPRRGLEFVTRKISDAVARIKLGMSKELRLGNLEAQRDWG
                     FAGDYVEAMWLMLQQNQPDSYVIGTGETHSVREFCEIAFSHLNLDYKQYVVQDEKFYR
                     PAEVDLLISDPSRARTELKWEPAVSFKELVTMMVDSDLARLKKG"
     gene            complement(7151..8737)
                     /locus_tag="PLE39_15745"
     CDS             complement(7151..8737)
                     /locus_tag="PLE39_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69835.1"
                     /translation="MKYFKKQDLIWMPPLALGLGAVLSLIQPGNWFFGWLESSLLFLL
                     CFLLLTVSARWANGASTVPDTSKTLAWMLAVAFALRFFGGVATYLALPVDGFADEDDK
                     AGYVYTDAHRRDGQAWELAGSEHPILDAFNKKFAYDQYGGLLAISAFVYRYISPDAHR
                     PLLLVLLASFAGTLCLSFLWKAVTLQWGQKVAVAAGWVFALYPEGVLLGGSSMREPYL
                     MLFSAFTLWGFVIWQKTQDKKTLIWFGLGILGMLLVSPVVALVTLALFAGWVYFTREH
                     SQLSWWGALAISVVFIAGLFWLASTLDRQGNLGGGTPLAVINNFLREAIKMDLHHLER
                     GSGWVQKLFDEMPKWLRLPFVLVYGVFQPVLPAAFVEPTTFTWRVIAIARAVGWYALL
                     PALILSFGAAAGQQSDEKRKLLVWLSLVAWFWVLLTSLRGGADQWDNPRYRTIMFLWQ
                     AILAGHVWVWWRESRNAWFLRVVAMEVVFLLVFGQWYASRYFHLGGQMQFAQMVAVIL
                     GGWVLILLGGWWQDRKKGNP"
     gene            complement(8815..10479)
                     /locus_tag="PLE39_15750"
     CDS             complement(8815..10479)
                     /locus_tag="PLE39_15750"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE69836.1"
                     /translation="MKKQTVLPFLVAVLVLSLACQTLTNPGAQASPTPPILFSTPETP
                     APQTGSAQLAGLTYATHDSGTDSNQLWQIGSDGKAVVISESGYAIFSPDGTQAAYIDF
                     NGQCSWLLDYEKKEARPLDCTGSNNPEFFELDEVIGWAAGQPDTLFMLFNESFGIGGG
                     TGYFGTLSTSTGEKRIFDNQHAVSTVLPSPDGRTVLFSNFDFAALYTLGSDSQPIDLG
                     QYKLGEFKLAYPAWSPDGKTIAWGLMNENGDLKYALGIMDPQTKTSKILHPYSPMLAP
                     VFADGPYVSTPQMSSPKPIWSPDGQWLAFEAYVYSQDQNNPQREAWVIRADGSAENKL
                     DGKFINWSPDGQWVLYFKPLPNTPSKADLMAAHPDGSNAARIAEVTFDEFLTQAVWSP
                     DSKHLLLRDGETVALTEAGSWQLNSLSGTEIPPDARLLAWLDSFPMPFSQLTVLPLPT
                     PSAASDFSCPTAPPTRLSVGATGRVTYTDGQTIRLRSAPEAGDNVIDQLPEGTEFEVI
                     DGPVCTPRIDRADAFVYWKVKVPTRNGISGWLAEGTFNAYFIEPWP"
     gene            10623..>10919
                     /locus_tag="PLE39_15755"
     CDS             10623..>10919
                     /locus_tag="PLE39_15755"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HNE69837.1"
                     /translation="MTPKVETSTPPHTPTPIGPYNHIAKVGTFITIGGTAGVDPATGK
                     LAGDDVFSQTVQILKSFQVMLESVGSDLDHVVHINIFLKNMSDFEEMNRAYISKM"
BASE COUNT         2934 a         2965 c         2924 g         2096 t
ORIGIN      
        1 gatgcggcgg cgatgaaggt caattgctca aacgcagtcg agcgatcctg cctgcggcgc
       61 atggcgcgaa cgcggcaaat cgtgacgccg tcgagggttt ccaaaaaagg ctggtcgccg
      121 aacagcgagg tcaacaccgt cacctcgtgc ccgagtgcgg caagacaacg ggcgatgttc
      181 gcgctggcat tgcctgcgcc gccgccgatg ggtggatact cgctgttgat gagcaaaatt
      241 cgcatggtta tttttcaaac gggtctttgc attcggggtt gttctcgcgc ggcgcaaaca
      301 cttgaatatt tgtcgaggat agagtcgtca caggtttata cgcgcacagg atcggtgcgc
      361 ccacaaaacg cacccacgca ttgttctctt ccaaaaacgc gccgtgtttt tgcagtgcaa
      421 atttttgttc ctcggcaacg atcagcgcga aacgatgcga tctcaggtca gcgtaaaaat
      481 tttcaagata gtcgcggttg cccgacatcg ccatttccat cagttccatc tgctcatatt
      541 cgggcacaag cggaatatta tttacatcgt tgaaaatcaa caactgacgt tcggttacaa
      601 aaaggatttc gccttcaaca ttttgtgtgg atgcctgcaa cttgaacagg tccgcttcga
      661 caaggtttga atcgtacctt tgaaagaatc caacctgtgg aagcgcgaat ccgatcggga
      721 cgagggcggc tgctgcaaca acccaaacag gaatcgcccc ccaggcagga ttcgcttcgc
      781 gctcccccgc tacccgcccg ccgatgaaag ccgtcacgac aagcgccagc atcacgagaa
      841 acgcatccat gttgtgcaaa tctgcgccgc cgccgatctt cacgctgacg accatgccgc
      901 cgagaaaaag aacagccagc atggcaatca atgccagcca gcgcaacgga tgcaaactgg
      961 aaatctttcc gcggaccatt tgaatgaacg caaagatcaa cggggcagag acgatgacaa
     1021 tccccaataa aattcccatc gggaaggttt cattcggaag caggcggctc cagatcaggt
     1081 cagatgtgaa actggaaccg aaggaacgcg catcactgtt gcctgaaacg gcgatgtacg
     1141 caaactgcga agcagacgca gcagcgagcc cgcagattcc ccataggaac ggggtcagcc
     1201 aataacgcca gccttttcct tccagcggtg tttcaagcag gtagatcgca accgccagca
     1261 tggcagggac gggaaaccag ttcacgcggc tggcacccgc ccaaacagaa gcgagaacca
     1321 cgaacagcaa tgaccgccac gggtgcttga ccgacacccc cgccaaaatc aaaatgacac
     1381 agacctgcaa atgataataa accgcgcctt ggaaaaaata caggaaggcc caggcaatga
     1441 gcgcgaatcg aacccaccct gtgaagttca agcggcggat caacaaaatg gaagaggcgg
     1501 cagtcagacc gatccacagc agcgcctgcc agaggcgatg cgcccaaagc ggcaaaccgt
     1561 ccaccagaaa tggaagcgac atcaaaaagt aacgcgtggg gtgcaagaat ggaagcgcga
     1621 gttcggttcc gtacagcggt ctagcaaaga acagcgaagc ataataatgc cgccccgctt
     1681 cagaataacc gatggaaaat ggatacgcag aaacgatggt gagatgcccg aaaatttgat
     1741 agaccacccc ctgcacaagc acagccgcca cgaacggggt gagccaatcc ctgcctgtca
     1801 gcaattgcaa gccaaaggtt tgaatcaaac ttgcccagag aaaaacccag aagatgggtg
     1861 caatttgcgg aaggacatcc ccaaaaaaga aaaggcgaac cgcccaaacg agaaagaagc
     1921 caaaggcaat caacacagac ccagcccacg ggttgattcc gcttttcgta acggcggcct
     1981 gtttttcggt caggtatgaa gcgcgggcag tggaaagctg ccacagcaca aacatccccg
     2041 caaaaatatt caacgcgaac aggcccacca gcagaatcca cttggatcgc cagagggtga
     2101 tctccaaatc agccacgcgc tgaacggtct ggtagatggc cagcccgctc atgaggactg
     2161 tggcggtgat gaatgtttgc cagaagattt tcgaggaggt tggtttcatt gcggaagcag
     2221 acctgcgtcg ttcaaaaatc gggcggatca attccccgcc ccgatatcag gtttatcttt
     2281 cgtcaacttc cttgctattc attgcggcaa ggatggaggg caggaagtat gcaccaggca
     2341 ggctggtgat gaggaaaata atgcgcacca atacggccac tgtggcgctt tctgattcgg
     2401 tcagcccgcc gaggctggtg agcaaaaacg tcatcgaaag ttcctgaaca ccgaggccgt
     2461 tgatggaaat gggaatcagg gtcacgaaat aagagagtgt gtacatgccc gccaccagcc
     2521 agaacgaaag gcggtgaccg atgcccagca aaagaaaata gaccatcaaa taaataaagg
     2581 cctgattacc cagcgtagcc agcagggaag caaacaaggc cgagggtttc ttcaaccaga
     2641 tcgaaagcga ttcgaaagtg cggcggataa agtccacgcc tttttgaagg agggcggata
     2701 atgtgacaga ttgggtcgaa ccgttcgaaa gcgcaaaaag cggaaccagc ccaaagggaa
     2761 gggccaatgc cattcccgcc atccccacca aacggtccac gacgagtgag gcgaggcaaa
     2821 tggcgcggtc ataaccaagc tgcatggcac cggtcaaacg caccacatcc ccgccgatgg
     2881 tggtcggcag gaaattggac gaaaagttgc cagtaaaggt gagttcaacc gagcggagga
     2941 aggaaatgtt cacacccgcc gagcgcagca ggatgtgcca gcgcaaaatg gcgaaaagcc
     3001 gcgaaacaag caagacgaca agagccagcc caaaataccc cagtgaaacg cggcggaagg
     3061 cggaaaacaa ttcaccatcc ccctgctcct gaatcaagac cgccagaagc accaacgcca
     3121 gaacgctgcc gatcccacgc gcaacggtct gacggttctt gctgagccag ttaatcacgc
     3181 ggatcctgct ccaggttgat catggtgcgg atggtatagg tgggtttgcg ctgtgactcg
     3241 tgatacgtgc ggacgagcag ttccgcgatc agacccagca ggatggattg gaagccgagg
     3301 atgaagaaca tcgcgctcat ctgcaacagc ggcgaattgg caatggcaac caaaaagaaa
     3361 atgcggcgca caaaaagata caacatgatg atggcgctga tgaccatgag aaacccgcct
     3421 gcgccgccaa acagataaat gggtttcgaa gaataggaga tcagaaattt tacggtaaac
     3481 agatcaagca cgacctttgc ggtccgctcc aggccgtact tggtcttgcc atgtttgcgc
     3541 gggtgatggt tgaccaccac ctcggtgatc ttcgccccga ccgaatgggc atagaccgga
     3601 ataaagcggt gcatttcgcc atacaagcgg aacccttcaa gcacgtcgcg gcgataggct
     3661 ttgagtgtgc agccgtaatc gtgcagatgc acccccgtca cctttgaaat gatgcggttg
     3721 gcgatcatgg atgggaaatt gcgggtgatg gcattatctt tgcggctctt gcgccaaccg
     3781 ctgacaaggt cataaccttc gtccagtttg gcaagcatca tggggatgtc agcagggtcg
     3841 ttctgcatgt ctgcatccag cagcacgatg atctcgccct gggcataatc gagcccggcg
     3901 gcaatcgcgg cggtctgccc gaagttgcgg cggaatgaga tcacacgcac atgagcaaag
     3961 tcttttttgg cgtactcgtt caaaacagcc aggctgttat cctgactgcc atcgtccacc
     4021 aaaatcacct cccatgtgcg attcatcgtg ggcatgacct tgttaatggc ttcgaacaaa
     4081 agcggcaggt tatcctgctc gttatagact ggaactacaa gtgaaagatt catggcagcc
     4141 tgtgaaatat ccttagttca gacccatcct cgcaagaggg ggccacgggc ggtttggggt
     4201 actcttcaaa ctcattcgga ttcaggaaat ccttccagag tgtgtagcgt ttggattgaa
     4261 tgatgaaaat atcggcactg gctttccatt cttccttcaa ttcggtattc cagtgactga
     4321 agcgggccag tgtatcggca tcgccgccga tgcggtacga ataaccgtcg ttgatctgcg
     4381 gcggaaagat acgcgcctgc ggcacgtagg tcatcgcagt aaacgacaga ccgccgtccc
     4441 aatataccag actatccgca gggatgaaac ttgcgagatg agcgccgacc tgttcgttgg
     4501 cgaccagcat atccgtttcg cagtcaatac tgcggccacc cacatgcagc agcggagaaa
     4561 ggatcagccc catcgcgatg gacaaattga gcaaaacgaa ggcaaacccc gggcgagtct
     4621 gtgcatcctt catgcggcgc gtgatgatgc ctgcaatcag cagaaccgca acacccacta
     4681 acaaacccag ggccgcagaa acataacgct tgacccagat caattccgtg ccgaacttat
     4741 tggtgataaa tgccagcagg gtggttgtac ccggcaggaa ttgtccgccg ctcatgcgcg
     4801 ggaccgggaa cttaaggaaa aactggctga ggctgtcgaa ggatgaaaat ccgaatcctg
     4861 tcgcaagggt gagcagggtc gctgcgccga ggaacaggat ccaacgggat ggagttcgat
     4921 tcgccaaatg cgagaggacg atcaccgtaa aaagaattcc cagcgggtcg aaaaacgaca
     4981 gatacggcgt gaagcagtac acacagctat agctttcata ctgactcgcc accgccgccc
     5041 aaccgtgcat ggcgaagagg atgaaatatg tcagggcgag gaacaaggcc gctcgcatgg
     5101 cagggacagt tttccagtcc cgtgggatgc gcaagatcat cagcgcaaaa atgctcccag
     5161 cggcgggaat gaaatggtag cggattccct gaaaaaacga aatcgtccga ttccaaaaat
     5221 caatggacgg atcccaaaca ggaaccgctt ccttcggcag gcggaacgga ttcaggaaag
     5281 gggtgagtcc atcgggcagc cacggcgccc aaatggtgag gatgttgggc cagtaataaa
     5341 tgtgaaccgc caaaaagatg gcggaagccg taccaaaaga aacgagccct ttttgtttgc
     5401 catgctgcca aaagacatac aggatgagca aaggcagaac cacgaccatg ttctgccgcg
     5461 tcagcacggc cagcgcacca agcaccgacc ctgccacgat ctgccacagc ggcctctcct
     5521 cagcaagcag gaagacgcac atccacgcca gcatgcaggc gatgatgacc tcggaagcgg
     5581 taatggcatg gatcttgatc accgtcggcg caagtgcata cgcccagacc gtggccgttg
     5641 caaaccaatt ccctgcccaa cggcgtgcgg tgatccacgt ccccaaaatg gcaagcaggc
     5701 cgagaaagat catgaaaaag cgccccgtgc gcagacctgc cccaaaaatg tattctgcaa
     5761 aaccggggat caaaaatgca aaaggagcct tattggtcag cggaccataa ggctcgaaag
     5821 gttcatacac gccgtgcaga taaagatatc ctttgaaaag ataggagcct tcatccaggc
     5881 cggggatggt ggtatgtgcg taacgccacg cctgtgccac atacagaatc accccaagaa
     5941 ttgcaagcaa acccggcacc caggattgcc aatcttttct gttgaaagtc gaaaaactta
     6001 accacggaga cacagaagcg cggagttttt ttgaactttc cccaatgtct tcgtgcctct
     6061 gtggttcaga acgggattca gtattgcgac cttgcattct acgcaagaga ccgaacagag
     6121 tactattcat agaaccaatg gaaaagggct accccttttt cagccgtgcc agatccgaat
     6181 ccaccatcat ggtgaccagt tccttgaatg aaacggcagg ctcccacttc aactctgttc
     6241 gcgccctgga aggatcggaa atcaacaaat ccacttcagc ggggcggtag aatttttcat
     6301 cctgcaccac gtactgtttg taatcaaggt ttaagtgaga gaaggcaatt tcgcaaaact
     6361 cacgcaccga atgggtctcg cctgtaccga taacatagct atcgggctgg ttctgttgca
     6421 gcatcagcca catggcctcc acgtaatcgc ctgcaaagcc ccaatcgcgc tgggcttcaa
     6481 ggttacccag acgcagctct ttcgacatgc ccagcttaat gcgggccacg gcatctgaaa
     6541 tcttgcgggt cacaaactcc aacccgcggc gcggactttc atggttgaac agaatgcccg
     6601 acgtggcaaa catattgaac gactcgcggt aattgaccgt gatccaatgt ccgtacattt
     6661 tcgccacccc atacgggctg cgcggataga acggtgtgga ttctctctgg ggaacttcca
     6721 tcaccttgcc aaacatctcg ctggatgaag cctgataaaa acggatcttc gggtctacga
     6781 aacgaatggc ttccagcagg cgggtcacac ccagagcggt cacatcacct gtcagcgcgg
     6841 gttgattcca tgaggtcggc acaaaagact gcgccgcaag gttatatact tcggtcgggc
     6901 ggtattcttc aaggaaagaa agcaaagacc cctgatcatg caaatcgccc tgtacaacgg
     6961 taatatcatc caaaatatgc tggatgcgtt cataggtaac cgtgctcgaa cggcgtgcaa
     7021 cgccaacaac acgatatccc ttcttcaaca aaaactcagc aaggtaggaa ccatcctgac
     7081 ctgtaatgcc agtgatcaat gcagtgggca tgttaactcc ttattcaaaa ttcagacgct
     7141 gaattataca ctacggattc cccttttttc ggtcctgcca ccagccgccc agcaaaatca
     7201 gcacccaccc gccaagaatg accgccacca tttgggcaaa ctgcatctgc ccgccaaggt
     7261 ggaaataacg gcttgcatac cactgtccga agaccagcag aaaaacaact tccatcgcaa
     7321 ccacacgcag gaaccacgca ttgcgcgact cacgccacca aacccagaca tgccccgcca
     7381 gaatggcctg ccacaagaac atgatcgtgc gataacgcgg attgtcccac tggtccgcgc
     7441 cgccgcgcag ggaggtgagc agaacccaga accacgccac cagactcagc cagaccagga
     7501 gcttgcgctt ctcatctgac tgttggcctg ctgctgctcc gaacgagagg atcagcgccg
     7561 gcagcaaagc ataccagccc acggcgcgtg caattgcaat gacccgccag gtaaacgtgg
     7621 ttggctccac aaaggcggcg ggtaaaacag gttgaaagac cccatacacc aacacaaaag
     7681 gcaggcgcag ccactttggc atttcatcaa acaacttttg cacccagccc gagccgcgct
     7741 ccaaatgatg caagtccatt ttgatggctt cgcgtaaaaa attgttgatc accgccagcg
     7801 gggttccgcc gccgaggttt ccctgcctgt ccaaagtgga agccagccag aacaacccgg
     7861 caatgaacac aaccgagatc gccagcgccc cccaccacga aagctgacta tgctctcgag
     7921 tgaaataaac ccagcccgca aaaagagcca aagtgaccaa cgccaccacg ggagaaacca
     7981 acagcattcc aagaatgcca agcccaaacc aaattaaagt ttttttatcc tgcgtctttt
     8041 gccaaatgac gaatccccac aaagtaaatg cactgaacag catcaagtag ggctctcgca
     8101 tcgaagaccc gccgagtaaa acaccttccg ggtacagcgc gaagacccac cccgcggcaa
     8161 ctgccacctt ctgcccccat tgcagggtta cagccttcca tagaaaggac aggcacaacg
     8221 tccctgcaaa ggatgcgagc aacaccagca gcaatgggcg gtgggcatca ggcgaaatgt
     8281 agcgatatac aaaggcgctg atagccagca ggccgccgta ttggtcgtag gcaaatttct
     8341 tgttgaacgc atccagaatc gggtgttcgg accctgccaa ctcccaggcc tgtccatcgc
     8401 ggcgatgcgc atcggtgtag acgtagcccg ctttatcgtc ttcatctgca aagccatcca
     8461 ctggcagggc aaggtaggtt gccacacccc caaagaaacg caaagcaaag gcaacggcca
     8521 gcatccatgc aagggtcttg ctcgtgtcag ggacagtcga agcgccgttt gcccaacgtg
     8581 ctgaaactgt cagcagcaga aaacaaagca gaaagaggag ggaagattcc agccagccga
     8641 aaaaccagtt accgggttga atgagcgaaa gaaccgcccc cagcccaagc gccagaggcg
     8701 gcatccaaat cagatcctgt tttttgaaat atttcattcc ccttttttac cacgaagtag
     8761 gcgagtctaa aagccgcaca ttcttggaca aacaaacctg cttgtgtttt ctctttacgg
     8821 ccacggttcg atgaagtagg cgttgaaggt accctcggca agccagcctg aaatgccgtt
     8881 gcgcgttggg accttgacct tccagtagac aaaggcatcg gcacggtcaa tgcgcggagt
     8941 gcagacgggg ccatcaatga cttcgaattc ggtgccttcg ggcagttgat ctatcacatt
     9001 atctcccgct tccggtgcgg acctgaggcg gatcgtctga ccatcggtgt aggtgactcg
     9061 gcctgtggcc cccacgctca accgtgtggg aggcgcggtg gggcaggaga aatcagatgc
     9121 ggcagaggga gtgggtaacg gaaggacggt caactgggag aaaggcatgg ggaaggaatc
     9181 cagccatgca aggagtcttg cgtctggagg gatctctgtc cctgagaggc tgttcaattg
     9241 ccaggagcct gcctcggtga gtgcgaccgt ttctccatcc cgcaacagca ggtgttttga
     9301 atccggtgac caaacggctt gggtcaaaaa ttcatcaaag gtgacttcgg caatgcgggc
     9361 cgcgttggag ccatcggggt gggcggccat caggtcggct ttggaggggg tgttgggcag
     9421 tggtttgaaa tacaaaaccc attgaccatc cggactccaa ttgatgaatt ttccatcaag
     9481 tttgttttct gcactgccat cggcacggat gacccacgct tccctttggg ggttgttttg
     9541 gtcttgactg tagacatagg cttcgaaagc cagccattga ccatcgggac tccagattgg
     9601 cttcggggaa gacatttgtg gtgtggacac atatgggccg tcagcaaaga cgggggccag
     9661 catgggggag tatggatgca aaatctttga ggttttggtt tgcgggtcca tgatgccaag
     9721 ggcatatttg aggtcgccgt tttcattcat caggccccat gcgatcgttt tgccatccgg
     9781 tgaccatgcg gggtaggcaa gtttgaattc acccagctta tattgcccga ggtcaatggg
     9841 ctgggaatcg ctgcccaaag tgtaaagcgc ggcaaaatcg aagtttgaga aaagcacggt
     9901 cctgccatcg ggtgagggca aaacggtgga aactgcgtgt tggttatcga aaatcctttt
     9961 ttcgccggtg gaggtggaga gggtaccaaa ataacctgtg ccaccgccta tgccaaagga
    10021 ttcgttgaaa agcatgaaca gggtatcggg ttggcctgca gcccagccga tgacctcatc
    10081 aagttcaaaa aattcggggt tgttggaacc cgtgcaatcg agcggtctgg cttccttctt
    10141 ttcgtaatcg agaagccacg agcattgacc gttgaaatcg atataggcgg cttgcgtgcc
    10201 atccggtgaa aagatggcgt atccgctttc agaaatgacc acggctttgc catcgctccc
    10261 gatctgccag agttgattgg aatcggtgcc cgagtcgtgg gtggcgtagg tgagcccggc
    10321 caattgcgca gagcctgttt gtggggcggg tgtttcaggg gtggaaaata aaatgggcgg
    10381 tgtgggagag gcttgtgcgc cggggttggt caaggtctgg caggcaagcg agaggacaag
    10441 aacggcaacg agaaagggca gaactgtctg tttcttcatg gctcctcctt gagcggtgca
    10501 attatattcc ccttttttat cggttttgcg atgttggcta aaaaatagct gcagaagttc
    10561 aactgcaaag atggttttca aaaacagcag ggtacaatgc aatcaaaaca caaggagtga
    10621 ccatgacccc caaagttgaa acttccacac caccccatac ccccaccccc atcgggccat
    10681 acaaccacat cgccaaagtt gggaccttca tcaccatcgg cggcaccgca ggcgtggacc
    10741 ctgccacagg caagctcgcc ggtgatgatg tcttttcgca aaccgtgcag atcctaaaat
    10801 cctttcaggt gatgctcgaa tccgtcggct cggatctgga ccacgtggtt cacatcaata
    10861 tctttctcaa gaacatgagc gacttcgaag aaatgaaccg cgcctacatc agcaaaatg
//
LOCUS       DAOQJC010000129        10809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_3139_length_10809_cov_8.432304, whole genome shotgun sequence.
ACCESSION   DAOQJC010000129 DAOQJC010000000
VERSION     DAOQJC010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10809
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3139_length_10809_cov_8.432304"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2326
                     /locus_tag="PLE39_15760"
     CDS             <1..>2326
                     /locus_tag="PLE39_15760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE69838.1"
                     /translation="SSSLVTYNGKTAIMSIARDISERMQAEEKIRESESRYQQFVSLA
                     LEAISRTEFENPINTSLPVEQQIDLVYENAYIAECNQAMADMYDTTVEALIGKRIVEI
                     HGGGDNLVNRDAIRKFIAGGYQSINNETKEYSVNGAPVWILNNTVGIVRDGMLLRLWE
                     TSIDITERKRAQEDLYLAEQRYRAMIEHAPDGIVLITVDGKFKYASPSVERIFGYVQE
                     DLQALNSVSMTHPDDLPKVLRELQKLLEDQSYIPTVQYRFLHKNGEWRWIESTFSNLL
                     ATPNVEAIIINFRDIHERRLADEELKKSQSLLKESQRIGRIGYMEWSVNSPNLICSDE
                     IYDIFNIPSGSPVTKDLISTMLRPDESEKLRELDHQATIHRADINYEYSVLTRDGNER
                     CIHQTGKITYDETGNPIRKMLIVQDITERKQAEKQLQRNEQVLRLFVEHSPAAIAMFD
                     REMRYIIASQRYLIDYRLGDQSLIGRSHYEVFPEMDEGRKAIHRRCLAGEVVKHEGDP
                     LPRVDGTLDWVRYELRPWYEVDGSIGGLIFFSEVITERKQAEEKLRASEEKYRGLIES
                     LGSIVATIDAHGKFLYLNDLAASSLGGSAAELTGRSMYDYFPPKYAESQMEAIRQVIE
                     SDKAVVFESQSYVQDELRWYRTSIQPIHDEDGKVSHALTNTTDINDIKTIQHELEVLN
                     RTLEEKINQRTAEVQDLYNNAPVGYHSLDVEGKYVQVNQTELDWLGYHREEMLGRSFA
                     EFVTAQDATLFQGTFQLLKNNGRVTGLEFNMRRKD"
     assembly_gap    2327..2426
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2427..3800
                     /locus_tag="PLE39_15765"
     CDS             <2427..3800
                     /locus_tag="PLE39_15765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE69839.1"
                     /translation="LKNNGRVTGLEFNMRRKDGSTFPVLLNATAVFDEHGNYLRSRST
                     IFDNTERKKAEDALRVSHDQLAAANIDLEKAIRVKDEFLASMSHELRTPLTGILGLSE
                     AIQLGTYGGLNEKQKKTLQSINDSGNHLLSLINDILDLSKIEAGKLEMQITPCSLGDI
                     CQSSLQLTKGMAHQKHQFVSYSPPAEAILLNVDARRAKQILVNLLSNAIKFTPENGEL
                     GLDVEPDAVNHRVRLIVWDKGIGIKAENLEKLFKPFTQIDSSLAREYSGTGLGLSLVQ
                     RLVKLHNGTVEVESVFGEGSRFIVSLPWMPDISASNSPKADSTQLTSSPSNPTAPDAS
                     PVILVADDNPVLLTMLADFLEASHYRAVKAGSGRDVLDNIAATRPGLVLMDIQMPGMD
                     GLMTIRHIRTHADPVVASTRIIAVTALAMAGDRERCLAAGANEYISKPVNLKELKQMI
                     ESQLGKL"
     gene            4023..5450
                     /gene="kaiC"
                     /locus_tag="PLE39_15770"
     CDS             4023..5450
                     /gene="kaiC"
                     /locus_tag="PLE39_15770"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HNE69840.1"
                     /translation="MNTQLLKTPTKIRGLDEVLNGGVPTGGLTLLNGGPGTGKTLLGL
                     EFLIRGAKERRPGIFVTFEEHEAALHRYSASFGWDTAELERQGLLSIIGARFDPEGLL
                     SGDFDLGGILAILRHKTEKLSARQVVIDAPDVFLRLLNSPGKERAELYKLINWLRDQK
                     MTTLMTVKTNGEDVTSSDYEFLDYLADCVIQLDQRVIEQVTTRRVRVVKFRGSAHGRN
                     EYPFAMTDQGIWIIPITETQLTHTALGEPFPTGVKGLDAHMGGGYLRASCNLVTGTSG
                     TGKTTFAASFARTSTANGKRVFYINFEESPDSMVSCMLSPGIDLRPALESGFLRFLSV
                     MPEAQGIEEHLIQAFRGIEELKPEIVIVDAISACRRMGSRNSAFDYLLRLIDHCKTIG
                     ITSLLTNLTDSIKETQEITGIDLSSMIDTVIILRNVEQNGQFQRELSVLKARGRLHSN
                     KIHAFQISDHGVSVINSTAEATRDH"
     gene            5440..5736
                     /locus_tag="PLE39_15775"
     CDS             5440..5736
                     /locus_tag="PLE39_15775"
                     /inference="COORDINATES: protein motif:HMM:NF019309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HNE69841.1"
                     /translation="MTINSQTNVRKKHYILRLFVAGNATNSRIARENLDQLLAKHPEH
                     EFEVEIVDLNLEPEVALEQGVFISPALQILEPNAGGVIYGNLSQMEVLEKVLNL"
     gene            5769..8888
                     /locus_tag="PLE39_15780"
     CDS             5769..8888
                     /locus_tag="PLE39_15780"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE69842.1"
                     /translation="MSQTKPTYQELEQRCQAAESALTAIRSGQADMVIGENGVLVLRL
                     KEAEKAFQEAELKYRSLAENSPDIIYIIDLKKNKSGYLNRETLLGYSLEELEKPGESL
                     LSKVHPDDLSAVKMHWQEALAGSTTTELEYRLQLKDGSWEWLHSRPRVLSWSAEGKPV
                     QLLVTLSIITERKQAERRLRSMLDIAQAMSQFLDTKTILQQIVDNITQVAGLDSGAIY
                     TLADDQLYLEATTPALPPEFPEQFRLANLADHPHIRAALAERSPVIISDTGNAELTEA
                     EREVSVSRGLRSIAYLPLMSARKVIGVLIVASAHGLRTFSEEELNLYKGFSGQAAQII
                     ENAQLYDSIRVHSNELEQQITERKQAEEKLRESEKLYRHAMEVAGAVPYYELYNENPP
                     AVKYEFIGEGIRQITGYGPEEFNAMVWDSLVEKAVPVEELEGFSLDEAIQRTRNGEFP
                     IWKCEFRLHDRDGKVHWVFEAAVELRDENGNPVGSIGSYQDITIRKQAEEKLRKSEEQ
                     YRGLMGTLDSVVATIDDSGKFLYMNEVAASQLGGAPHQLIGRSLHELFPEPLAFQYLQ
                     GVHNVIQTNKSMVNETLTIVDGGARWFRNSLQPIHDENGNVIQVLLHAVDIHELKTIQ
                     QELADLNKSLERRVEERTVEVRQANQALERALLAKDEFLASMSHELRTPLTGILGFSE
                     AMQMNVYGILNERQQKIVGSINSSGKHLLDLINDILDLSKTEAGKLEIQKSPTLLADI
                     CLASLQLTKGMSHKKGQYVNTVIPEEPIILDLDMLRIKQVLVNLLSNAIKFTPENGEL
                     GLTVETDEANRLVRVIVWDKGIGIKPENFPKLFQPFTQIDGSLAREYVGTGLGLSLVR
                     RLVELHDGSVTVESVFGEGSRFIVTLPWITKISPSVSEHTETAWQASPKSVPANLRPL
                     GKVLIVDDNPSLLEMLTDFLEGKNFSSASVRSGIELLEKIEEIKPDVILMDIQMPGMD
                     GLEAIRRIRAFEDRSIASIPIIAVTALAMRGDRELFMAAGANGYVSKPIKLNELVETI
                     QSQLGGSKQ"
     gene            complement(8926..9840)
                     /locus_tag="PLE39_15785"
     CDS             complement(8926..9840)
                     /locus_tag="PLE39_15785"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNE69843.1"
                     /translation="MHKLIILSEYEKKYQALIEKAQLPNLQITGLLANDVDIAIGEPT
                     RLKAALAYLPGLKWAQSLWAGVEPLLDPALRRDYVLTNARGVFGGLMSEYVLGYLLAH
                     ERRIFQMAEDQKNKHWNDSDTGTLRGKTIGLLGVGSIGADVARTAKFFGMTVRGYTFS
                     SEASSDVDKYYHGNDLLEFAKGLDYLVNILPNTKDTRKIINADLLTALPPHALVINVG
                     RGPAVDEAALLEALSRQKVAGAVLDVYEKEPLPQDHPFWSTPNLHMTFHTAALSLPED
                     LTRIFLENYNLYNEGKPLKYQVDFEKGY"
     gene            complement(9843..10643)
                     /locus_tag="PLE39_15790"
     CDS             complement(9843..10643)
                     /locus_tag="PLE39_15790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69844.1"
                     /translation="MIELSLNEAQAARAIFAPLEHHTMIYALFENNLKARLFVDNASQ
                     PTAGMIAYKNRYAFGGDPNQSAFHADILQHFAETELPPRKGRAFLASFTTDAWIPTLQ
                     NLFPDDEVIVAPRLYLEITPEQNTPPALPDGFSLHAVTPELLGSNIGGMELLEEEIHS
                     ERTSPEDFFANGFGICPVYENQIAGWCLSEYNTHDRCEVGIATLEPHQRKGIATTLTK
                     IFLNEAAQRGIRHVGWKCWERNEASVATARKAGFSLVHREQAVIVIYK"
BASE COUNT         2733 a         2475 c         2875 g         2626 t
ORIGIN      
        1 agttccagtc tggtcacata taacggcaaa accgccatta tgagcattgc ccgtgacatc
       61 agtgaacgca tgcaggcgga ggagaagata cgggaaagcg agtctcgata tcagcaattt
      121 gtctcccttg ctcttgaggc gatctcacgt acggaatttg aaaacccaat taatacatcc
      181 ctgcctgttg agcagcagat cgaccttgtt tatgagaatg cctatattgc cgaatgtaat
      241 caagccatgg cggatatgta tgacacgacg gtcgaagccc ttattggaaa gcggatcgtt
      301 gaaattcatg gtggtggaga taacctggtt aatcgcgatg cgatcagaaa gttcattgct
      361 ggcgggtacc aatccattaa taacgagacc aaagagtatt cagtcaatgg agcaccagtt
      421 tggatattaa ataacacggt tggcatcgtc cgcgatggga tgcttttacg tttgtgggag
      481 acttcgatcg atattaccga gcgaaaacgc gcccaggaag atctgtatct ggctgagcag
      541 cgctatcggg caatgatcga gcatgcaccg gatggtattg ttttgatcac tgtggatgga
      601 aaattcaagt atgccagccc atcggtggag cggatatttg ggtacgtgca ggaagacctg
      661 caagcattaa actctgtatc catgacacac cctgatgacc ttccaaaggt attgagggaa
      721 ttgcaaaaat tgttggaaga tcaatcttac atcccaactg tgcaataccg ttttttgcat
      781 aagaacgggg agtggcgctg gatcgaaagc acattcagca acctgcttgc aaccccaaat
      841 gtggaagcca tcatcattaa ctttcgggat atccatgagc gcaggctggc agatgaggaa
      901 cttaagaaga gccaaagcct gcttaaggaa tcccagcgta tcggacgaat cgggtatatg
      961 gaatggagcg tgaattctcc aaacttgatc tgttcagatg agatttatga cattttcaac
     1021 atcccgtctg gaagccctgt tacaaaagac ttgatcagca ccatgttgag gcctgatgaa
     1081 agtgaaaaat tgcgggaatt ggaccatcaa gcgaccattc atcgtgccga tataaactat
     1141 gagtattcag tccttacaag ggatggaaat gaacgatgta ttcatcaaac aggtaagatc
     1201 acctacgatg aaactggcaa cccgatccgc aaaatgctta ttgtgcagga tattaccgaa
     1261 cgtaaacagg ccgaaaagca attgcagcgc aatgaacagg tcctgcgttt gttcgtggag
     1321 cacagcccgg cagccattgc catgtttgac cgggaaatgc gctacatcat cgcgagtcag
     1381 cgctacctga tcgattatcg gcttggtgac cagtccttga taggacgctc gcactacgaa
     1441 gtatttcctg agatggatga aggtcggaag gcgatccatc gtcgctgcct ggctggggag
     1501 gtggtaaaac atgaaggaga cccgctccca agagttgatg gaactttgga ttgggtccga
     1561 tatgaactcc gcccctggta tgaggtggat ggcagtattg gcggtctcat ctttttctcg
     1621 gaagtgatca ccgaacgcaa gcaggctgaa gaaaagttgc gcgccagtga ggaaaaatat
     1681 cgaggcttaa tagagagcct gggcagcatc gtagccacaa tcgacgcaca tggaaaattt
     1741 ctatacctga acgatcttgc agccagcagt cttggcggat ccgctgctga attgacaggc
     1801 aggtccatgt atgattactt cccgccaaaa tatgctgaaa gtcaaatgga agcaataagg
     1861 caggtaattg aaagcgataa ggcggtggtg tttgaatcgc agagctatgt gcaagatgaa
     1921 ctgcgttggt atcgtacttc cattcaaccg atccatgatg aggacgggaa ggtttcgcat
     1981 gcattaacta atacgaccga tattaatgat atcaaaacca tccagcacga gttggaggtg
     2041 ttaaaccgta ccttggagga aaaaattaat caacgcactg ctgaagtgca ggatttgtat
     2101 aacaacgctc cggtggggta tcactctttg gatgtggagg gcaaatatgt tcaagtgaac
     2161 cagacggagt tggactggct gggataccat cgcgaagaaa tgctggggag gtcttttgcc
     2221 gagtttgtaa cggcacaaga tgccacactg ttccagggca catttcagtt gttaaagaat
     2281 aatggcaggg taaccgggct tgagtttaat atgcggcgca aggatgnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnttaa agaataatgg cagggtaacc gggcttgagt
     2461 ttaatatgcg gcgcaaggat gggtcaacat tccctgtgct gctcaatgca acggctgtat
     2521 ttgacgagca tggaaactac cttcgcagca gatccaccat ctttgataac actgaacgta
     2581 aaaaagctga agatgcactt cgggtcagcc atgatcagct ggctgccgcc aatatcgatc
     2641 tggaaaaggc gatcagggtc aaagatgaat tcctggccag catgagccat gaattgcgca
     2701 cgccgcttac aggcatcctc ggtctttccg aagccattca acttgggact tatggcggct
     2761 tgaacgaaaa acaaaaaaag accctccaaa gcattaatga cagcggcaat catttgttgt
     2821 cgctcattaa tgatattctg gatttgtcga agatagaagc tgggaaattg gaaatgcaga
     2881 tcacgccgtg ttcgcttggt gatatttgcc aatccagctt gcagcttacg aaaggcatgg
     2941 cccaccagaa acaccaattc gtcagttatt ctccgcccgc cgaagccatc ctcttgaacg
     3001 tggatgcaag gcgagcaaaa cagatcctgg ttaatttatt gagcaatgcg atcaagttca
     3061 cacctgaaaa cggcgagctt gggcttgatg ttgagcccga tgcggtcaat catcgggtac
     3121 ggctgatcgt ttgggataaa gggatcggga tcaaggcgga gaaccttgaa aaattattca
     3181 agccattcac gcagatcgat agcagcctcg cccgtgaata ttccggtaca ggccttggcc
     3241 tttcgctggt gcagcggctt gtgaaattgc acaatgggac cgtggaagtg gaaagcgtct
     3301 ttggagaggg cagccgcttt atcgtttccc tgccctggat gccagacatt tccgcttcaa
     3361 actcacccaa agcggacagc acccaattga catcttctcc ctctaaccca actgcccctg
     3421 atgcttcccc tgtgatcctg gttgcagacg acaaccccgt acttctcact atgttggccg
     3481 attttttgga ggcgagtcat tatcgtgctg taaaggcagg gagtggcagg gatgtcctcg
     3541 ataatatcgc tgccacccgg ccaggcctgg ttttgatgga tattcaaatg cccggcatgg
     3601 acggtctcat gacgatccgc cacatccgta cgcatgccga cccggttgtt gcatccacca
     3661 ggatcatcgc agtgactgcc ctggccatgg ccggggatcg ggagcgctgc cttgccgcag
     3721 gagctaatga atacatcagc aagcctgtca atttgaaaga actaaaacag atgatcgagt
     3781 ctcagcttgg aaagctgtaa atcattccat ctctgaagtt agggcattgt gggcgggtcc
     3841 atttttaggt gtgtatggcc atcgacctca aagcttttcc tgtccttttt agatatttca
     3901 ctgatagaca taattggtta agttgtataa aataatttac attactggca tactggtttt
     3961 attacttatg gcaaaaacca cggtttcttt gtaacgtttg cgcataaaat caggaggtgt
     4021 ttatgaatac ccagttgcta aaaactccca ctaaaataag ggggcttgat gaagtcctca
     4081 atggaggggt gcctaccggc gggctgaccc tactcaatgg cggacctggt accggtaaga
     4141 ctttgcttgg gttggagttc ctgattcgag gcgcgaaaga gaggcgtcct gggatttttg
     4201 tcacatttga agagcatgag gcggcgctgc accgatattc cgcctcattc ggttgggata
     4261 ccgctgaatt ggaaagacaa ggactccttt cgatcattgg agcgcggttc gacccggagg
     4321 ggctgctttc cggtgatttt gatctggggg gaatcttggc gatcttgcgc cataaaacag
     4381 aaaaactttc agccaggcag gtggttattg atgctcccga tgttttcctt cgtctgctca
     4441 atagccccgg caaggaacga gctgaattgt acaaactgat caactggctg cgtgaccaaa
     4501 agatgacgac tcttatgacg gtcaagacaa atggggaaga tgtaaccagt tccgactatg
     4561 aatttctcga ctatctggca gattgtgtga tccagcttga ccagcgtgtg atcgaacagg
     4621 tcaccacccg aagggtgcgt gtggtcaaat tccgcggttc tgcacatggg cgaaatgaat
     4681 atccgtttgc catgaccgat cagggtatct ggatcatccc gattacagaa acacagttga
     4741 cccacactgc tttgggcgaa ccgttcccaa ccggcgtcaa aggtctggat gcgcacatgg
     4801 gcggcgggta tctgcgtgcc tcatgcaacc ttgtcaccgg cacatctggt acaggcaaga
     4861 ccacctttgc tgcatctttt gcgcgcacat ccacggcgaa cggcaagcgt gttttctaca
     4921 ttaactttga agaatcgcct gactcgatgg tgtcatgcat gctcagcccc ggcattgatt
     4981 tgcgccctgc cctggaaagc ggcttccttc gattcctgtc tgtgatgcct gaagctcagg
     5041 gaatcgagga gcatcttatt caagcattcc gcgggatcga agaactaaaa cccgagatcg
     5101 tgatcgtgga tgccatctca gcctgccgcc ggatgggatc gcgtaattca gcgttcgatt
     5161 atctattgcg cctgatcgat cactgcaaga ccatcggtat taccagcctg ctgaccaacc
     5221 tcactgacag catcaaagag actcaagaga tcaccggtat tgacctttcc tcgatgatcg
     5281 atactgtgat cattttgcgt aacgtggagc aaaatgggca gttccagcgg gagctatcag
     5341 tgctcaaagc aagagggcgc ctgcattcca ataagataca tgcgtttcag atttcagatc
     5401 atggggtctc cgtaataaat tcaactgcgg aggcgacccg tgaccattaa ttctcagacg
     5461 aatgttcgaa aaaagcatta catcctgcgc ctgttcgtgg cggggaatgc caccaattca
     5521 cggatcgcac gtgagaacct cgatcaattg ctggcaaaac accctgaaca tgaatttgaa
     5581 gttgaaatcg ttgatctcaa cctggaaccc gaggtggccc tggagcaggg tgtgttcatt
     5641 tcacctgccc tgcaaatcct tgaaccgaat gcaggcgggg tcatatatgg caatctaagt
     5701 cagatggaag ttctggaaaa ggtcctgaac ctttgaattg tttattgcat ggaaatggag
     5761 cgtgatcctt gagccagaca aaaccgacct atcaggaatt ggaacaacgc tgtcaagctg
     5821 ccgagtcggc actgactgcg attcgctcag gacaggcaga catggtcatt ggtgagaatg
     5881 gggtattggt tttgcgcctg aaagaggcgg aaaaagcctt tcaggaagca gaattaaaat
     5941 atcgctctct ggcagaaaat tcaccggaca ttatttacat cattgacctg aaaaaaaata
     6001 agtctggcta cttgaaccgc gaaactctat tggggtactc tctggaggag ttggaaaaac
     6061 cgggagagtc cctgttatcc aaggtgcatc ctgatgatct ctctgctgtt aaaatgcatt
     6121 ggcaggaagc tcttgcaggc agtaccacaa cagaattgga ataccgctta cagttgaaag
     6181 acggcagttg ggaatggctt cacagccgcc cacgtgtttt gagttggagc gcggaaggca
     6241 agccagttca gttattggtc acacttagca taattactga acgaaaacag gcggaaagac
     6301 gtttacggtc catgctggat attgcccagg cgatgtctca gttccttgat acaaaaacga
     6361 tcttgcagca aattgttgat aacattaccc aggtggctgg gctggatagt ggagcgatct
     6421 acacactggc agatgaccaa ctttatctgg aggccaccac ccctgccttg ccgcccgaat
     6481 ttccagaaca attccgtttg gcaaatctgg ctgatcatcc ccatattcga gcagccctcg
     6541 cagaaagatc acccgtcatc atatctgata ccgggaacgc cgagttgacc gaagccgagc
     6601 gcgaggtgag cgtttccagg gggctgcgtt caatcgccta tttaccgctc atgagtgcca
     6661 ggaaggtgat cggggtcttg atcgtggcat ccgcccatgg gctgcgtaca ttttccgagg
     6721 aggaattgaa tctttataag gggttctctg gtcaggcggc acagatcatt gaaaatgcac
     6781 agttatatga ttccattaga gttcattcca atgaactgga acaacagatt actgaacgta
     6841 aacaggcaga ggagaaattg cgggaatcgg agaagttgta tcgtcatgcc atggaggtgg
     6901 ctggggctgt gccctattac gagttgtata acgagaatcc tcctgctgtt aaatatgagt
     6961 ttatcggaga ggggatccgt cagatcaccg gctacggacc tgaagaattt aatgcaatgg
     7021 tttgggattc actagtggaa aaagcagtac cggtggagga actggaaggg ttttctttgg
     7081 atgaagcgat tcaaagaacc cgtaacggtg aatttcctat ctggaagtgt gaattccgcc
     7141 ttcacgatcg tgatggaaag gtacattggg tctttgaagc ggcagtggaa ctgcgggacg
     7201 aaaatggaaa ccctgtaggt tcgattggtt cttatcaaga tatcaccatc cgtaaacagg
     7261 cggaggagaa acttcggaag agtgaagaac aataccgtgg tttgatgggt actctggata
     7321 gcgttgttgc caccattgat gacagcggca aattcctgta tatgaacgag gtcgctgcaa
     7381 gtcaactggg cggtgcccca catcaattga ttggtagatc gttgcacgaa cttttccccg
     7441 aacctctggc tttccaatat ttacaggggg tgcataacgt gattcaaacg aacaagagca
     7501 tggttaacga aactctcacc attgtagatg gtggagcccg ctggtttcgc aattcgctcc
     7561 agccgatcca cgatgaaaat gggaatgtaa ttcaggtgct gcttcatgcc gttgatattc
     7621 atgaacttaa aacgatacag caggaattgg cagacctgaa taaatcgctt gaacgacggg
     7681 tggaggagcg cacagtggaa gtgcgtcagg caaatcaggc attggaacgt gctttacttg
     7741 cgaaagacga gtttctggca agtatgagtc atgaactgcg cacgccgctt accggcattc
     7801 tcggcttctc cgaagccatg cagatgaatg tctacggtat attgaatgaa cggcagcaaa
     7861 agattgttgg aagtataaac agcagcggaa aacacctgct cgacctgatt aacgatatcc
     7921 ttgatctttc caaaaccgag gctggtaagt tggaaattca aaaatcgccc accttgttgg
     7981 cggatatttg cctggcgagc ttgcagttga ccaaaggcat gtcccataaa aagggacagt
     8041 atgtcaatac cgtcatccct gaagaaccca ttattctcga tctggatatg ctgcgtatca
     8101 aacaggtcct tgtcaacctg cttagcaatg ccatcaagtt cacccccgag aacggcgaat
     8161 tagggttgac tgttgagaca gatgaagcca accggctggt tcgtgtgatc gtatgggata
     8221 aaggtattgg catcaaacca gagaatttcc ccaaactatt tcaacctttc actcagatcg
     8281 atggcagtct ggcacgagag tatgtcggca caggtttggg gctttcactg gtgcgccgcc
     8341 tggtggaact tcatgatgga agtgtgaccg tcgaaagcgt tttcggtgaa ggcagccgct
     8401 ttattgttac cttgccatgg ataacgaaaa tatccccgtc tgtttcggaa catactgaaa
     8461 ctgcatggca ggcttccccc aaaagcgtcc ccgcaaattt gcgtccactt ggcaaggttc
     8521 tgattgtaga tgacaaccca tccctgcttg aaatgttgac agattttctc gaaggaaaaa
     8581 atttcagttc cgccagcgta cggagcggta ttgaactgtt ggaaaagatc gaagagatca
     8641 agccggatgt gatcttgatg gatattcaaa tgcccggcat ggacggtttg gaagccatcc
     8701 gacggatccg tgcctttgaa gacagatcca tcgcttccat tccgatcatt gccgtcaccg
     8761 cccttgccat gcgaggcgac agggaacttt tcatggccgc tggtgcaaat ggatacgtca
     8821 gcaagccaat taagttgaat gaacttgtgg agaccattca gtcccaactt ggagggtcga
     8881 aacaataaaa aacaggaatt gggtacatgc ccaattcctg ttttttcaat accctttttc
     8941 aaaatcaacc tgatatttca gcggcttgcc ttcgttatac agattgtaat tctcgaggaa
     9001 aattctggtg aggtcttcgg gcaggctgag ggcggcggtg tggaaggtca tgtgcaggtt
     9061 gggcgttgac caaaatggat gatcctgcgg aagcggctct ttttcgtaga cgtccaaaac
     9121 ggctcctgcg accttttgcc tgctcagcgc ttcgagcagg gcagcttcat ccacagcggg
     9181 accacgaccg acgttgatga cgagggcatg cggcggcagg gcggtgagta gatccgcatt
     9241 gatgatcttg cgcgtgtctt tggtgttggg gaggatgttg acgagataat caagtccttt
     9301 tgcaaattcg agcaggtcat tgccgtgata atacttgtcc acgtcagaac ttgcttcgct
     9361 tgagaaagtg tacccgcgca cggtcatgcc gaagaatttc gccgtccgcg ccacatccgc
     9421 gccgatggag ccgacgccaa gcaagccgat ggtctttccg cgcaatgtgc ctgtgtctga
     9481 gtcgttccag tgtttgttct tctggtcctc tgccatttgg aagatgcggc gttcatgtgc
     9541 cagcagatac ccaaggacgt actcagacat gagtccgccg aagacgccgc gggcattggt
     9601 gaggacgtag tctctgcgga gggcagggtc aagcaaaggc tccactcctg cccatagaga
     9661 ttgagcccac ttcaagccgg ggagataggc gagcgcagct ttgaggcggg tgggttcgcc
     9721 gatggcaata tccacatcgt tggcgagcag tcccgtgatc tgtaaattcg gaagctgcgc
     9781 cttttcgatc aaagcctgat attttttttc gtattcagag aggatgataa gtttgtgcat
     9841 ggctatttgt agatgacgat cacggcttgt tcgcggtgga cgagggaaaa gcctgctttg
     9901 cgcgcagtgg caaccgaggc ttcgttgcgc tcccagcact tccagcccac gtgacggatc
     9961 ccgcgctgtg ctgcttcgtt gagaaaaatt ttggtgagcg tggttgcgat gcctttgcgt
    10021 tgatgcggct ccagcgtggc gatgcctact tcgcagcggt cgtgcgtgtt gtattcggag
    10081 aggcaccagc ccgcgatttg attctcgtac acggggcaaa tgccgaagcc gttcgcaaaa
    10141 aaatcttctg gcgatgtacg ctcggaatga atttcttctt ccagtaattc catcccgccg
    10201 atgttgctcc ccagcaattc gggcgtgacg gcgtgcaggg aaaatccgtc tggcagggcg
    10261 ggcggtgtgt tttgctcggg tgtgatctcc agatacaggc gcggggcgac gatgacttca
    10321 tcatcgggaa aaagattttg cagggtgggt atccatgcgt cggtggtgaa agaggcgagg
    10381 aacgccctgc ctttgcgcgg agggagctcg gtctctgcga agtgctgcag gatgtccgca
    10441 tggaacgcgc tttgattcgg gtcgccgcca aacgcgtagc ggttcttgta agcgatcatc
    10501 ccagcggttg gctgggatgc gttatctacg aatagcctcg ccttgagatt gttttcaaac
    10561 aaggcgtaga tcatggtgtg atgctcgagc ggggcgaaga tcgcgcgcgc tgcttgtgct
    10621 tcgttcagtg agagttcaat cattagcatt ccttcaaaac ggtttcaaag cgggcgagcg
    10681 catcatcaat gacttcgtcg gtatctgcca ttgaggtgta catgcgcgag cctgccagtg
    10741 tgatgagccc gtttgccatg taggctgcgc ccatttcttc catcatgtgt ttgcgcggtt
    10801 tgagttctt
//
LOCUS       DAOQJC010000130        10800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_3143_length_10800_cov_9.779898, whole genome shotgun sequence.
ACCESSION   DAOQJC010000130 DAOQJC010000000
VERSION     DAOQJC010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10800
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3143_length_10800_cov_9.779898"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PLE39_15795"
     CDS             <1..615
                     /locus_tag="PLE39_15795"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE69845.1"
                     /translation="YKFFGPHAGILFGKHELLEKLFAYKVRPATNKLPGKLETGTQNH
                     EGIAGVLGAIEYFEWVGREFGGEHAESFAGSGYTGRTLTLKKAMTAIRAYEHEMNRAL
                     LSTLEAVPGITIFGLTDTRRLDERVATFSFRLKNLHPRKTAELLAEKGIYVWDGNYYA
                     INVSERLGFEGNGGMVRVGAAHYNTLDEIARLGDVLKEIAEKAA"
     gene            643..1653
                     /locus_tag="PLE39_15800"
     CDS             643..1653
                     /locus_tag="PLE39_15800"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNE69846.1"
                     /translation="MVFQIVLTTVLALVFSFVIAPLSISLAWRFHLIDDPGLAARKIH
                     KAPMPRAGGTIIFLAILSGGALSGVLTTRPFTSIILALMIVFAFGIWDDIHNIRAIWK
                     LLGQILATLILIWQGVVVLFPGNTALNIAITLFWVIGITNAFNLVDSMDGLAVGLAGI
                     TVSFLMIGALYAGQNTLALFCAILLGASISVFHLNFSLSRLFLGDSGTQLLGFTLAAI
                     AIVYTPPHLPQASSWFVPILLFGIPIFDTTLVTFSRMRKKMPVYKGNRDHTFHRLVDL
                     GATPHRAVLSIHMVAILLDCLAFFALSLSPLWANITFGVILLSGLFAILWLEANQKVE
                     TL"
     gene            1669..2568
                     /gene="speB"
                     /locus_tag="PLE39_15805"
     CDS             1669..2568
                     /gene="speB"
                     /locus_tag="PLE39_15805"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNE69847.1"
                     /translation="MTVEGTHGLWGDLHRPDLDPKQADFSVVGIPFDGLASARKGAAL
                     APERLRYWSRHLTPFSEDRTRLKDITLCDLGDISIVNPATDFNMIRQRVATLPNIPIM
                     LGGDHSVSIPIFQGQRERYQDKRLGVLWIDAHPDLCDTFDGSKLSHACVLRRGIEFGI
                     EPHDICMVGLRSWEDQEIDIVESGGVHVYTAADVAERGIRKVADSVRNILNDCDAIHV
                     SLDIDCLDPSAAPGTGIPEFGGMTSRDVLTLLKSMQGLPLVGLDVVEIAPPLDPSEAT
                     VFAGLKIIMEFIAVIAREKQKGL"
     gene            2570..2854
                     /locus_tag="PLE39_15810"
     CDS             2570..2854
                     /locus_tag="PLE39_15810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69848.1"
                     /translation="MPANSLNVTHNTDKNRFEVQIDSLTAELVYRLNGNVILFLHTGV
                     PPALEGRGIGSLLVKTGLQYAREKNLKVQSLCWFVSGYIERHPEFQDLVG"
     gene            complement(2908..3390)
                     /locus_tag="PLE39_15815"
     CDS             complement(2908..3390)
                     /locus_tag="PLE39_15815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNE69849.1"
                     /translation="MKTLVVGLGNPILGDDGVGWKVAEEVRAQLSEAGLDVDVECLSL
                     GGISLMEHLIGYSRVILVDSFALDEPIGSILILKLDDLPNYSAYHTASPHDTSLQTAI
                     ALGKTMGAWLPDEVTVVGIATKRIHEFSEELSPPVAEAVPLAGKFVMELIREKMNIEE
                     "
     gene            complement(3434..>3880)
                     /locus_tag="PLE39_15820"
     CDS             complement(3434..>3880)
                     /locus_tag="PLE39_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNE69850.1"
                     /translation="THWARLVELLYAAERWVELVTDPEITSADIHSKPTQIPTEGIGI
                     VEAPRGTLTHHYWTDELGRVTKVNLIVGTTNNYAPIQMSTHKAATSLIKDGVVNEGLL
                     NMVEMAFRAYDPCFGCATHSLPGQMPLEITVRDVDGNPVRVLSQFC"
     assembly_gap    3881..3980
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3981..4774)
                     /locus_tag="PLE39_15825"
     CDS             complement(<3981..4774)
                     /locus_tag="PLE39_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNE69851.1"
                     /translation="MTQRITIDPITRLEGHGKIEIFLDDKGNVANSYFQIPELRGFER
                     FCVGRPIEEMPLLTNRICGVCPEAHHMAASKAADMVYQVDPPRTAKLLRELLYSAFYC
                     TDHTTHFYALGGPDFVMGPDAPVAERNILGVIHKVGLEIGGKVIQMRKYGHTVVEMIG
                     GRKVHPCTSIPGGMTKGITEEQRKEIEEMGRWAIDFAQFSLKIFNDIVLGNKAYVDLI
                     LGDVYTHHTYYMGMVDKNNKINFYDGDVRVVDPNGKEFVKYAPKEYA"
     gene            complement(4863..>5139)
                     /locus_tag="PLE39_15830"
     CDS             complement(4863..>5139)
                     /locus_tag="PLE39_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69852.1"
                     /translation="SILGKENDMNTKPIENVEPVEDPNALLEKALIEEYLKEKGYSHE
                     DLKKLPADLVEKLMKEASQYASLKMEEVEARAHFVKELHDDASSLEK"
     assembly_gap    5140..5239
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5244..6242)
                     /locus_tag="PLE39_15835"
     CDS             complement(5244..6242)
                     /locus_tag="PLE39_15835"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-nonreducing hydrogenase"
                     /protein_id="HNE69853.1"
                     /translation="MTTKPKFAMYWAASCGGCEIAVLNTHEKILDVDANFDVVFWPVA
                     MDAKYHDVEAMEDGSILLTLFNGGIRNDENEHIAKLLRQKSKILVAFGSCACEGCIPG
                     LANLSPVGDIVHTAFHTITTDNPNEIYPRTSYDVPEGELHIPTLSRVVRPLEQVVDVD
                     YFMPGCPPESHQIAAVIDLVIKVVKGEAELPPKGSVIGCGDSTVCDECPRARNVKFIK
                     EFKRIQDVAPVDPTLCLLEQGIPCNGPATRSGCNARCPQAGAQCIGCYGPAEGVMDYG
                     ARLITAFSSVIDANTPEEIDHILDGIPDPTGQVYRFNLAGSLLKANREAWKVVSGK"
     gene            complement(6239..>6721)
                     /locus_tag="PLE39_15840"
     CDS             complement(6239..>6721)
                     /locus_tag="PLE39_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNE69854.1"
                     /translation="FEPTIIGFTCNWCSYRASDLAGTARMKYAPNVRLIRLMCSGRLD
                     PTFVLRALSDGADGVMITGCHPGECHYIEQNYKALRRYLLLKRVLVGMGIEPGRVKLV
                     WASAAEGAKFAAETSAFVEEVRKLGPLNLNAECKMQNDESENSSFINPHSSFEKEVHA
                     "
     assembly_gap    6722..6821
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6822..8786)
                     /locus_tag="PLE39_15845"
     CDS             complement(<6822..8786)
                     /locus_tag="PLE39_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013099797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HNE69855.1"
                     /translation="MTERVGVYVCHCGSNIAGTVDVVDVANWAAEKLGRQGVIIAREY
                     KFMCSSLGQELIEKDIKELGLTRVVVAACSPHLHEKTFRTAAKNAGLNPYLVELASIR
                     EQVSWVHSDKAVATAKAKAVVSGAVSRVIENRPLEEIKVPINPNTLVVGGGIAGIQSA
                     LEIANAGFHVYLVEREPSIGGHMAQFDKTFPTLDCSACILTPRMVEAGTHPNITLLTW
                     SEVTNVAGYVGNFHVTIKKKARFINEELCTGCGICQEKCPKKVIDDVYEAGLGYRKAV
                     YTPFAQAVPNFPVMDKENCTYFIKGTCKACEKFCPTGAIDFNQQDEMLHIDVGNIILA
                     TGYDLFDARRVSNYGYGRLPNVFTSLEFERLSNAAGPTSGNIVLRDGKTVPKSVGIIH
                     CVGSRDKNFNNYCSVICCMQGLKFAHLVHERTGATVYNFYIDMRTAYKAYDEFYQRVL
                     EEGTLFVRGKVAEVTNAARNEREKGRLIIQCEDTLAGKQRRIPVDMVILSSGLQPRKD
                     AKEVGKMFGISCSMDGWFTEKHPKLDPVATMTEGIYIVGCAQGPKDIPSSVAQGAAAS
                     ARVLDKILQKEVALEPIKASVNQELCSGCRICNGLCPFNAITFIEDDNVTSINPALCQ
                     GCGTCVAACPAGAISGTGFSNEQIIAQIDGL"
     gene            complement(8783..9781)
                     /locus_tag="PLE39_15850"
     CDS             complement(8783..9781)
                     /locus_tag="PLE39_15850"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HNE69856.1"
                     /translation="MNKYLLYPGCSMDSSAKAYMESLNSIVEPLDLHLEEIEDWNCCG
                     ATEYLGINLIPAYSLIARNLALASKQMDGGTRTVVAPCSACYLNLAKADYYMQERPTL
                     GAKVNEALAAGGLEYKAGTLDVRHLIDVLVYDVGLDAIRKAVTHPLSGLKVVPYMGCM
                     LPRPDYQHRWSDHEHPTELDDLLRALGADVIDYPLTTSCCGGHMTQIGPETAFELIHR
                     LVSDADERGAQMMVTVCPMCQMNIDAYQNEMNHFFGTDFHMPILFFTQLMGLAFGKAP
                     EELGIGVELVSSRNALAHIGVEVPVTQEPAAPRKKDEGLPMPRRWTKHEGKKEAVK"
     gene            complement(9778..10374)
                     /locus_tag="PLE39_15855"
     CDS             complement(9778..10374)
                     /locus_tag="PLE39_15855"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNE69857.1"
                     /translation="MPQMTDERVRAGMTREEDLSLLHEVSMKTAGVSHLEMCIQCGTC
                     GGSCPVAADMDHTPRMLFAMLRAGMREEVLRSNTPWLCVSCYHCVVRCPQEVHIADVM
                     YTLKGMAIHAKLYTDSTAPHFSQTFVDMVETFGRGYELGLATRHYLRHFPLRLPGMMP
                     MGFGMISKGRMAITPKKIKHMDQLTKILDRAKELELTS"
     gene            10556..>10800
                     /locus_tag="PLE39_15860"
     CDS             10556..>10800
                     /locus_tag="PLE39_15860"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE69858.1"
                     /translation="MNIFDLFENNVRNFPNKPAFIYFDKRKWITLTYQDIFDKTQRFI
                     RGLETGRYTPGITAALLTPPSADFFPFALALLKLGIVP"
BASE COUNT         2378 a         3033 c         2500 g         2589 t
ORIGIN      
        1 tataaattct tcggtcctca tgcgggcatt ttgtttggta aacatgaact gcttgaaaaa
       61 ttatttgcct acaaagtgcg cccagccacc aataagctcc ccggtaaact cgaaacaggg
      121 acgcaaaacc atgaaggcat tgcaggtgtt cttggtgcca ttgaatattt cgaatgggtt
      181 gggcgtgaat ttggcggcga acatgccgaa agttttgcag gcagcggata caccgggcgg
      241 accctgacct tgaaaaaagc catgaccgcc atccgtgcct atgaacatga aatgaaccgc
      301 gcgcttcttt ccacactgga agctgttccc ggtataacga tctttggtct caccgataca
      361 cgccgcctcg atgaacgtgt ggcaaccttc tcatttcgtt tgaagaatct ccacccgcgc
      421 aaaaccgcgg aattgctcgc cgaaaaaggg atctatgttt gggatggcaa ctactatgcc
      481 attaacgtca gcgaacggct cggctttgaa ggtaatggcg gcatggtacg ggttggcgct
      541 gcccattaca acaccctgga tgaaatcgcc cgccttggag acgtattgaa ggaaatcgcg
      601 gaaaaagccg cctaaggtgc gcatttcgta aacaaggtgc tgatggtttt tcaaattgtc
      661 cttacgaccg ttcttgcgct ggtcttttct tttgtcattg cgccgttgag catttctctt
      721 gcatggcgtt ttcatctgat cgatgacccc ggtctcgctg cccgcaagat ccacaaagcc
      781 cccatgccac gtgcaggtgg cacgatcatt tttctggcga ttttgtcagg cggagcattg
      841 agcggagttc tcacgacccg cccatttact tccatcatcc ttgctttgat gatcgtcttt
      901 gcctttggca tttgggatga tatccacaac atccgcgcca tttggaaatt gctcgggcaa
      961 atccttgcca cgctgatttt gatctggcag ggagtggtgg tgcttttccc cggaaacacg
     1021 gctttgaaca tagccatcac acttttctgg gtgatcggca ttaccaatgc cttcaatctc
     1081 gttgatagca tggatgggtt ggcggttggg ctggcgggca tcaccgtcag ctttttgatg
     1141 atcggcgcgc tgtatgcggg gcagaataca ctggctttgt tttgtgcgat ccttttaggg
     1201 gcatccatca gcgtcttcca tcttaatttt tcactctccc gtctgtttct gggcgattct
     1261 ggtacgcagc tgcttggctt tacccttgcc gccattgcga ttgtgtacac accgccgcac
     1321 ctgcctcaag cctcttcctg gttcgtgccc attttgctgt tcggaatccc catttttgac
     1381 acgaccctgg tcaccttttc gcggatgcgc aaaaagatgc cggtgtataa aggcaaccgt
     1441 gaccacacct tccaccgcct cgtggatctg ggagcaacgc cgcaccgtgc cgtgctttca
     1501 atacacatgg ttgccatcct gctcgattgt ctggccttct ttgctttatc cctctcccct
     1561 ttgtgggcaa acatcacatt tggggtcatt ctgttatccg gtctgttcgc cattctctgg
     1621 ctggaagcaa atcaaaaagt ggaaaccctc taaaaaggag attgaacgat gacagttgaa
     1681 ggaactcatg gtttatgggg tgacctgcat cgccctgacc tcgaccccaa acaagccgat
     1741 ttcagtgtgg tgggcattcc ctttgacggg cttgccagtg cgcgcaaagg tgccgccctt
     1801 gccccggaac gcctgcgcta ctggtcacgg catctgacgc cattcagcga agaccgcacc
     1861 cgcctgaaag atatcaccct gtgtgacctc ggcgatattt ccatcgtcaa ccctgcaacc
     1921 gatttcaata tgattcgtca gcgggtggct acattgccaa atattccgat catgttgggc
     1981 ggcgatcatt ctgtttccat tccgatcttt caaggacaac gtgagcgtta tcaggataaa
     2041 cgtcttggcg tgttgtggat cgatgcccat cccgacctgt gtgatacctt cgacggttcg
     2101 aaactctccc atgcctgtgt gctgcgccgc ggaatcgaat tcggcattga gccgcacgat
     2161 atttgcatgg tgggcctgcg ctcatgggaa gatcaggaga tcgatatcgt cgaaagcggc
     2221 ggcgtgcatg tgtacactgc cgccgatgtc gctgaacgcg gtattcgcaa ggtggctgac
     2281 agcgtccgca atattctcaa cgattgtgat gccatccatg tctcgctcga cattgactgc
     2341 ctcgacccat ctgccgcgcc cggtacaggc atccctgaat ttggcggcat gacctcacgg
     2401 gatgtgctta ccctgctcaa gtccatgcaa gggcttccgc tggtggggtt ggatgtggtt
     2461 gaaattgccc ctccgcttga cccatccgaa gccactgttt ttgcgggtct gaagatcatc
     2521 atggaattca tcgctgtgat cgcgcgcgaa aaacaaaaag gcttgtgaaa tgccagccaa
     2581 ttcattgaac gtgacgcaca acacggataa gaaccgtttt gaagtccaaa tcgattcgct
     2641 gaccgctgaa ttggtgtacc gcttgaatgg gaatgtcatc ctgtttctac acacgggtgt
     2701 gccgcccgcg ctcgaaggca ggggcatcgg cagcctgctt gtaaaaacgg gcttgcaata
     2761 cgcaagagaa aagaatctca aagtgcagtc tttgtgctgg tttgtcagcg gctacattga
     2821 acgccatcct gagtttcaag accttgttgg gtaacagagc aatccacgaa atgtaaaaca
     2881 aaagccatct gcaaagatgg cttttgttta ttcttcgatg ttcattttct ccctgatcaa
     2941 ttccatcacg aattttcctg cgagcggaac tgcctctgca acaggcggtg aaagttcctc
     3001 actgaattcg tgaatgcgtt ttgtcgcaat gcccaccacc gtcacctcat caggcagcca
     3061 ggcgcccatt gtttttccca atgcgattgc agtttgcagc gaagtgtcgt gtgggctggc
     3121 ggtatggtac gcggaataat ttggaagatc atccagcttc aatatcaaaa tggatccgat
     3181 cggttcatcc aatgcaaaag aatctacaag gatcacacgc gaatagccga tcaagtgctc
     3241 cattaaactg attccgccca acgaaagaca ttccacatcc acatccagac ccgcttcaga
     3301 gagttgcgct cgcacctctt ccgcgacctt ccagcctacg ccatcatcgc caagaatggg
     3361 attcccaaga ccgaccacca acgttttcat tggatgaaac tccgcttcac tcttaaccgt
     3421 tgcctgattc tgctcaacaa aactgactca acacccgaac cggattccca tccacatccc
     3481 gaacggtgat ctccaaaggc atttgtccgg gcaatgaatg ggtagcacac ccaaagcacg
     3541 ggtcgtaggc gcggaacgcc atctccacca tgttgagcaa accttcattc acaacgccgt
     3601 ctttgatgag actcgtcgct gctttgtgcg tggacatctg gatcggcgca tagttgttgg
     3661 tggtgccaac gatgagattc accttggtca cacgccccag ttcatcggtc cagtaatgat
     3721 gcgtgagcgt gccgcgcggc gcctcgacaa tgccgatccc ctccgttgga atttgagtcg
     3781 gcttgctgtg aatatccgca gaagtgatct cagggtcggt caccagttcc acccagcgtt
     3841 cggcggcgta cagcaattca accaacctcg cccaatgcgt nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn gcatactctt tcggcgcgta cttgacgaac tcctttccat
     4021 tcgggtccac cacgcggacg tcgccatcat aaaagttgat cttgttgttc ttatccacca
     4081 tgcccatgta ataggtgtga tgggtgtaca cgtcaccgag gatgagatca acataggctt
     4141 tgttgccaag cacaatgtcg ttaaaaatct tgaggctgaa ttgtgcgaag tcaattgccc
     4201 agcgtcccat ctcttcgatc tctttgcgct gctcttcggt gatgcccttg gtcatgccgc
     4261 cagggatgga tgtacacgga tgcaccttgc gcccgccgat catctccacc acggtgtgcc
     4321 cgtacttgcg catctggatc accttgccgc cgatctccaa tcccactttg tggatcacgc
     4381 caaggatatt tctttctgca acaggcgcat caggtcccat cacaaaatct ggcccgccca
     4441 atgcgtagaa gtgagtcgtg tggtcggtgc aatagaacgc tgaataaagt aattcacgca
     4501 gaagttttgc cgtgcgcggc ggatctacct gatagaccat atccgccgct ttggatgccg
     4561 ccatgtgatg cgcttcgggg caaaccccac agatgcggtt cgtaagcaat ggcatctctt
     4621 cgatgggacg ccccacgcag aaccgttcaa acccgcgcag ctcgggaatc tgaaaatagg
     4681 aattggcaac attccctttg tcatctaaaa atatctcgat cttgccgtgt ccttcgagac
     4741 gtgtgatggg gtcaatggtg atacgctggg tcatggaaac tcctttggaa tgcagaagtc
     4801 agaatgatga atgaggaatg aactattgtc gaagccattc accattctgc attcatcctt
     4861 catcatttct caagcgatga agcgtcgtca tgcaattctt tgacgaagtg cgcccgcgct
     4921 tcaacctctt ccattttgag ggatgcatat tgcgatgcct ctttcatcaa tttttcaaca
     4981 aggtcggcag gcagtttttt gaggtcttca tgcgagtagc ctttctcttt gagatattct
     5041 tcaatgagag ccttctccaa caatgcgttg ggatcttcca caggttccac attctcaatc
     5101 ggtttggtgt tcatgtcatt ctcctttcca aggatcgaan nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnc tttctacttt ccactcacca ctttccacgc ttctctattc
     5281 gccttcaaca gcgaccccgc caaattaaat cgatacacct gcccagtcgg gtcagggatg
     5341 ccatccagaa tatggtcgat ctcttcgggg gtattcgcgt caatgacgga tgaaaaagct
     5401 gtgatgagtc tcgcgccgta atccatcaca ccctcggcgg gaccgtaaca gccaatgcac
     5461 tgcgcccccg cctgcggaca tcgcgcattg catccgctgc gtgtggcggg accgttgcac
     5521 ggaatgccct gttcgagcag gcataaagtc gggtccacag gggcgacatc ctgaatgcgt
     5581 ttgaattcct tgatgaattt cacattccgc gcccgcggac attcatcgca cacggtcgaa
     5641 tctccacatc cgatgactga tcccttcggc ggcagttccg cttctccctt cacaactttg
     5701 atcaccagat caatgaccgc cgcaatctgg tgactctcag gcgggcagcc tggcatgaag
     5761 taatccacat ctacaacctg ttcgagcgga cgaaccaccc gcgaaagggt tgggatgtgc
     5821 aattcacctt cgggcacgtc ataacttgtg cgcggataaa tttcattcgg gttgtcggta
     5881 gtgatggtgt ggaacgccgt gtggacaata tctcctacgg ggctgaggtt cgccaaccct
     5941 ggaatacatc cttcgcaggc gcatgacccg aacgcaacga gtatctttga tttctgccgc
     6001 aacagcttgg cgatgtgttc attctcatcg ttgcggatgc cgccgttgaa gagagtcaac
     6061 agaatcgagc cgtcttccat cgcttccaca tcgtgatatt tcgcatccat cgccacaggc
     6121 caaaagacga catcaaagtt cgcatccaca tccaggatct tttcgtgggt attcagcacg
     6181 gcaatttcac atccaccgca agaggcagcc cagtacatgg cgaattttgg ttttgtggtc
     6241 atgcgtgaac ctccttttca aatgatgaat gcggattgat gaacgatgaa ttttcagatt
     6301 catcattctg cattttgcat tctgcattta aatttaatgg ccccaacttc cgcacctctt
     6361 ccacgaatgc cgatgtctcc gccgcgaact ttgccccttc cgctgcgctt gcccaaacga
     6421 gtttgacccg tccaggctcg atgcccatgc ccacgagcac acgcttgagc aagagataac
     6481 ggcgcagggc tttgtaattc tgctcgatgt aatggcactc cccagggtgg cagccagtga
     6541 tcatcacgcc gtctgctccg tctgaaagag cacgcagcac aaaggtaggg tcgaggcgtc
     6601 ctgaacacat caagcggatg agtctcacgt tcggcgcata cttcatgcgc gccgtccctg
     6661 ccaagtccga tgcgcgatag ctgcaccaat tgcaggtaaa gccgatgatg gtgggttcaa
     6721 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncaacccatc gatctgtgca
     6841 atgatctgct cattgctaaa gcccgtgccg ctgatcgcac ccgcaggaca agccgccacg
     6901 cacgttccgc agccctgaca cagagcggga ttgatcgagg tcacgttgtc atcttcaata
     6961 aaggtgatgg cattgaacgg acatagtcca ttgcagatgc ggcagcctga acaaagctcc
     7021 tgatttacac tggctttgat cggctcaagc gcaacttcct tttgcaggat cttatccagc
     7081 acacgcgccg aagccgccgc cccttgcgcc acgctggatg gaatatcttt cggaccttgc
     7141 gcacagccca cgatgtaaat gccttcggtc atcgtggcaa cggggtcgag tttgggatgc
     7201 ttctctgtga accaaccatc cattgagcag gaaatgccga acatcttgcc aacttctttt
     7261 gcatctttgc gcggctgcaa tccgctggac aggatgacca tatccacagg gatgcggcgc
     7321 tgcttgcctg ccagcgtgtc ttcgcattga atgatgagcc tgcccttttc tctttcattg
     7381 cgcgccgcat tcgtgacctc agccactttg ccgcgcacga agagagtccc ttcttcaagc
     7441 acgcgttgat agaactcatc gtaggctttg tacgccgtac gcatgtcaat gtaaaagtta
     7501 tagaccgttg cacccgtgcg ctcgtggaca agatgcgcga acttcaatcc ctgcatgcag
     7561 cagatcaccg agcaataatt attgaaattc ttatcgcgcg accccacgca atggataatg
     7621 cccacgctct tcggcacggt ctttccatca cgcaaaacaa tatttcccga agtcggcccc
     7681 gccgcattgg acaagcgctc gaactccaag ctcgtgaaca cattcggcaa acgtccatag
     7741 ccgtaattgc tcacgcgtcg tgcgtcgaac aggtcatagc cagtggcaag gatgatattg
     7801 ccaacatcga tgtgcagcat ttcatcctgc tggttgaaat cgatcgcgcc cgttggacag
     7861 aatttctcgc aagccttgca tgtgcctttg ataaagtagg tgcaattctc tttgtccatc
     7921 acagggaagt tcggcacggc ttgcgcgaac ggcgtgtaca ccgccttgcg atatccaagc
     7981 cccgcttcgt acacatcgtc gatcactttc ttcgggcact tctcctgaca aattccgcag
     8041 cccgtgcaaa gttcttcgtt gataaaacgc gccttctttt ttatagtcac atggaagttg
     8101 cccacatagc cagccacatt cgttacttca ctccatgtca gcagcgtaat gttcgggtgt
     8161 gttcccgcct ccaccatgcg tggagtgaga atacacgcgg aacaatccag cgtggggaaa
     8221 gtcttatcga actgcgccat gtgcccgcca atggacggtt cacgttccac gagatacaca
     8281 tggaatcccg cattcgcaat ttcaagcgcc gattggattc ccgcaatgcc gccgcccacc
     8341 accaaagtat ttggattgat cggaactttg atctcttcca gcggacggtt ctcgatcact
     8401 ctcgaaaccg cgcctgatac aacagccttg gcttttgccg tcgctacggc tttatctgaa
     8461 tgcacccacg aaacctgctc gcggatcgaa gccaactcca ccaaatacgg attcaatccc
     8521 gcattcttcg ccgccgtgcg aaacgtcttt tcatgcaagt gcggtgaaca cgccgccacg
     8581 accacacgcg tcaatcccaa ttccttaata tctttctcga tcaattcctg tcccaaactc
     8641 gaacacatga atttatattc acgcgcaatg atcacaccct gccgcccaag tttttccgcc
     8701 gcccaatttg cgacgtcaac gacatccaca gtgcctgcga tgttcgaacc gcagtgacag
     8761 acatacactc caactcgctc tgtcatttca ccgcctcctt cttcccctca tgcttggtcc
     8821 agcgacgcgg catgggcaac ccctcatcct tctttctcgg cgcggcgggt tcctgcgtca
     8881 caggcacttc cacgccaatg tgcgcgagtg cgttgcgtga gctgaccagt tccacgccaa
     8941 tgcccaactc ttcaggcgct ttgccgaatg ccagccccat caactgcgtg aagaacagga
     9001 tcggcatgtg aaaatccgtg ccgaagaaat gattcatctc attttgataa gcatcgatgt
     9061 tcatctggca catcgggcag accgtcacca tcatctgcgc gccgcgttca tccgcatcag
     9121 acacaagcct gtggataagt tcaaacgcag tttcaggccc gatctgagtc atgtgcccgc
     9181 cgcagcaact ggtcgtgagc ggataatcga ttacgtccgc acccagtgcg cgcagcaagt
     9241 catccaactc cgtgggatgt tcatggtctg accagcggtg ttgataatca ggccgcggca
     9301 acatgcagcc catgtacggc accaccttga gtccgctcaa cgggtgggtc actgcctttc
     9361 ggatggcatc cagccccacg tcgtagacaa gaacgtcgat caggtgacgc acatccagcg
     9421 tgcccgcttt gtattccaat ccaccagccg caagcgcctc attcactttc gcgcccaagg
     9481 ttgggcgttc ctgcatgtag taatctgctt tggcaaggtt gagataacaa gccgaacacg
     9541 gcgcaacaac ggttcgcgtg ccaccatcca tttgctttga agccaatgca agatttcgcg
     9601 cgatcaacga atacgcgggg atcaagttaa tgccgagata ctccgtcgcg ccgcagcaat
     9661 tccaatcttc gatctcttcc aaatgcaggt caagcggctc aacgatggaa ttgagcgact
     9721 ccatatacgc cttcgcacta gagtccatgg aacagcctgg atacaaaaga tatttgttca
     9781 tgaggtcaac tccagttcct tggcgcggtc caagatcttc gtcaattgat ccatgtgttt
     9841 gattttcttc ggcgtgatcg ccatgcgccc cttcgagatc atcccaaagc ccatcggcat
     9901 catgcctggc aagcgcagcg gaaaatgccg cagataatgg cgtgttgcca acccaagctc
     9961 ataaccgcgg ccaaaggttt ccaccatgtc cacaaaggtc tgcgagaaat gcggcgcggt
    10021 cgaatcggtg tacagctttg catggatcgc catccctttg agcgtgtaca tcacatccgc
    10081 aatatgcacc tcctgcggac agcgcaccac gcagtgatag cacgacacac acaaccacgg
    10141 cgtgttgctg cgcagcactt cctcgcgcat gcctgcccgc agcatcgcga acaacatgcg
    10201 cggcgtgtga tccatatccg ccgccacagg gcaggaaccg ccgcacgtcc cgcattggat
    10261 gcacatttcc aaatgcgaaa cgcccgcagt tttcatgctc acttcatgta ataaagaaag
    10321 gtcttcttcg cgcgtcatcc ccgcgcgcac acgttcatct gtcatctggg gcatagtgcc
    10381 tccgtgctac aactcaacca accagttgct gctcggcctg accgaagtca gccattcgca
    10441 gttaatgtat ggacatcccc catattcatc atagtatgag ggtttgcaaa taacagtaac
    10501 gaagataatc gtacaatcct gacaaacatc cctgacaagc gatacaattt ctaacatgaa
    10561 catttttgat ctttttgaaa ataatgtgcg aaattttccc aacaaacccg cctttatcta
    10621 cttcgataaa cgcaagtgga taacgctcac ttatcaggat atcttcgaca aaacccagcg
    10681 cttcattcgt ggacttgaaa ctgggcgcta caccccaggc atcaccgccg ccttattaac
    10741 tccccccagc gcggacttct ttccctttgc cctcgctttg cttaagctcg gcatcgtccc
//
LOCUS       DAOQJC010000131        10700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_3191_length_10700_cov_10.721090, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000131 DAOQJC010000000
VERSION     DAOQJC010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10700
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3191_length_10700_cov_10.721090"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PLE39_15865"
     CDS             <1..988
                     /locus_tag="PLE39_15865"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNE69859.1"
                     /translation="DSPKKFPLWNIAKIILALILVWFVLSKTDLTQLYALRERISLPW
                     LALTLLLYFSLTLLKALQYYFLIGRRVDYPQVLNIVVVQNAISNFIATSAGIASYLAL
                     FRVEQGVKVSRAALAFLLAKVGDLISIWLFMLVSSLLVWPLVASFHSLILLSLSFIGV
                     AILVFFAAVFLRQKFVSVLAWFVEKLKLSRLGFVNKLMDLLASLAEQEHGFVFRMVGT
                     GIAFSLIYMTVTMLWLYASLQTFSFEIQFLQVVFVNIFMQLISYLPIQVFGGLGVNET
                     TSLYLYGIFNFPQTELAATLIGTRLLFYLTNLVVLLYLPVHTLFFSRSAQKQ"
     assembly_gap    1185..1284
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1285..3236
                     /locus_tag="PLE39_15870"
     CDS             <1285..3236
                     /locus_tag="PLE39_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69860.1"
                     /translation="EQDQPFGWSDLALAFSIFLYPRIVQEIRQYLPAPLLTRGSIAVG
                     FVFQFALLAALYESVGKSLLAKLIELRARLEPVRLILAALFLLAPFIYRYSTGPKEYI
                     LYFNIRFMVALIALWFAAYLLCKDSSRLISPESFGVAAGWLVLVSAITRSLLLVVDDP
                     FGLYWSEGNRLYDYSLVFGQDLYNYAGRIPDPYNTPGRYGLWGVLYLWQGLPIWAHRL
                     WNVALLTVPSFVLAWALTKKLSPSFLRQMVFLWVTAFFILLAPLHPPFMIMTVIVALF
                     IFHPSPYVRGASLVAASLYAGISRWTWVFAPGAWGALTDLLLFYPKREGNWFKRLLPT
                     VLMAVLGAVPGFLLNIGNFLGYTTGENTTAQQPLLWYRLLPNLTLGPGIVLLILLTTG
                     PLLVLLAYKMSTRQWKLDGFQLLAVWGALIGFFTIGLVISTKIGGGGDLHNLDMYIGT
                     LIVVTMLGLTGQVEGQDVKRWSSSALTMVCLLAILPIYPFTPFHPSAGSHAWLDLPTR
                     EDTDKALKVINDSVANYSQAGEILFMDQRQLLTFGYVTAIPFVPEYEKKYMMDQALAS
                     NETYFRPYYQDLANKRFALIVTEPLKVDLKEEGGVFSEENDLWVKWVSAPTLCFYEQV
                     HADKTVGIALLVPRENPVGCEKYLK"
     gene            3298..4119
                     /locus_tag="PLE39_15875"
     CDS             3298..4119
                     /locus_tag="PLE39_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69861.1"
                     /translation="MNLSYLLPRILRHFMPESVARFLLKRRWIIRPGLESSDPFAAVE
                     QYMAGITASGKSLIGKRVLVFGYGGRFAVGVELLRRGAAHVVLCDHFVLLDKERNLEL
                     LPVYPEYLVGENDNVRPRAEYITLLHGDIREDAIQKQITEVDVVLSTSVYEHLDDVKG
                     ITHALAKLTASNGFHLHFVDLRDHFFKYPFEMLTFSEGVWRNFLNPTSNLNRFRLGNY
                     REVFDAYFRKVEIKILERLEPEFRAIRARIRPEFLTGDEAVDSVGLIQVIAEMRK"
     gene            4164..5807
                     /locus_tag="PLE39_15880"
     CDS             4164..5807
                     /locus_tag="PLE39_15880"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNE69862.1"
                     /translation="MQQRFSSFIIRNEKLIPVFIFLLFLAASLPGVDWGVPALWNPDE
                     MIWRVDSALRGEIIFDETEPDFNYPSLPKYVMFGIGKIVYGLGGTPSDFYISARSFSA
                     LLGAVAGVLVYFMARSIGVDVLTSALAGLLYVASGVAAANGRFAHNDLYLQLFSVLCV
                     YFVIKYQFTKARLWLYGSFFSVGLAASSKFTGGSLLLVPLFVFVFMNWSEVRRNWLQS
                     FEMLFIGGALSFGGYILGTPKALLWMAYYFKRVIPALQRYPQYGFNSGTPIGLFGQWP
                     VFEDAVGTFAYYIFILCFIWFVAKIILWKLGRLQMDETQAQAVMILAATVLLFDLPFM
                     ISINYIPRYFIPFVPFLSILGALFIKEIVEIAKAKNWKYVPQIVSVLLVVGITYSMLR
                     LASTALLFMNDARMPASEFVANLPGEGKTIEYTLYPPIVNKKQFAKAYNYPVFFLKYP
                     TDVVPTGGRYEYNQGEAGLIDRETDYLIIDTLTYGRLYTESVCVTNPVECDFFKRLVA
                     GEIKTFRLMKEFTYTLPPWLPDISLSAVNPEVRIYERVR"
     gene            complement(5854..6495)
                     /locus_tag="PLE39_15885"
     CDS             complement(5854..6495)
                     /locus_tag="PLE39_15885"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE69863.1"
                     /translation="MPPKLKLLPASQYKGVNQYDPIKYYYWPVFGKMYRQRVELALGE
                     CTGGERVLEVGFGTGLAFPNLHDMYKEIHGIDLTADIHAVKSVFEPTGIPLFLEKGDV
                     LKMPYQDNFFDTVLLVSILEHLKPAELKQAFAEVNRVLKPGGQMVYGTPVEKPFMVFM
                     FSLLGHNIRDEHFSTEKEIEEAAHKAFSKGHVIHMQSTPPIAGSVYTVGNFVK"
     gene            6576..6935
                     /locus_tag="PLE39_15890"
     CDS             6576..6935
                     /locus_tag="PLE39_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNE69864.1"
                     /translation="MLFEDMRQWRIMVLMIVVETHIFTRQVLLLLSDEEYRLLQAVLV
                     NRPESGVIIPSSGGLRKMRWGFLGKGKRGGVRVIYYWAVGQERILMLLIYPKNVKDDL
                     TREQLKILRNIVKEEFP"
     gene            6932..7228
                     /gene="nadS"
                     /locus_tag="PLE39_15895"
     CDS             6932..7228
                     /gene="nadS"
                     /locus_tag="PLE39_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HNE69865.1"
                     /translation="MKEDLFNELVVSVREGGAILRGEKKPARAFKITAPNIKRIRAGY
                     KLSQQEFAALLGISVATLRNWEQGRRMPEGPARVLLQVAARYPDVVWDVVKPIR"
     gene            7258..9777
                     /locus_tag="PLE39_15900"
     CDS             7258..9777
                     /locus_tag="PLE39_15900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE69866.1"
                     /translation="MPFSSSKKWSALFAVWILHGIVALWQFLSLPSEGFSLQRIVMLG
                     LLLAWVAFATFLAFSAFRQFHWLNGLLLRINKPFIRDGIFIAAVLALFVRISLMFLLG
                     LAAQTPEFHYGAYVERLSPFLDLLAFVAFEIIVLNLFFVFREGTEYFGLIKSISAKLF
                     IGLVLIGGVVLYISKTDMGIAPIYKGDWARGLPAVPLLEWQIFLAVLFCIGMFLAEVD
                     GRLLKIPSLDLWIALAVWVATVGLWLSQPTVPNASALEPREPNYEIYPFIDAHTYDKF
                     AQSVLIGNGFGPNEIPQRPLYIVFLVFMHTVVGQNYENVIALQTVFFAAFPVVLYIFG
                     REFFGRPIGVSLALLAALRDFTSNLVSPITGNISYSKLYLSEIPTAICLILFLLIGIR
                     WIRSGFPLYLGFLLGGVLGIGMLNRTQVVVALPVLLFFAFILQPKKLLFIIKGTLPML
                     AVIALVVAPWLWRNWQMTGKIIFDNPASQTANLALRYNRLNGEDVDILQKPGESNSDY
                     NDRMVAMANHAMSVNSQGIFKAIASSFINHGINNILLFPLRNDLATPAELITPTTPFW
                     QQWEGRPNFLQSLLLAFYVFLFSLGVTMACQRNGWLGLLPLMVNLAYNLWTSIALLSG
                     QRFMLSMDWSIAMYDLLGIFALLSIFFYALENGRAKILKWYEANTVSIIPTLEAIKWP
                     RYVFAGLMFFAVGASLPLSEMSFSQRYPALEQNQPADLTLLSSLAQTDLEPACQKLAE
                     SSGLEIVRGRALYPRFYSAGDGEIFTDSVGYKKVDEGRLVFDLLGQRNHRVIFPMSQS
                     PEFFPNASDVTIWFDKNASPWFILVEQGDAQRFYVSDRVCK"
     gene            complement(9843..>10700)
                     /locus_tag="PLE39_15905"
     CDS             complement(9843..>10700)
                     /locus_tag="PLE39_15905"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE69867.1"
                     /translation="IRPMREVLWPLPLGGLADRYLVKLWPFRDFALSNFVIARPAATP
                     AKNPSVSVIVAARNEAGNIKNIFERLPKMGSKTELIFVEGHSRDNTFQTIQDEIPLHP
                     STPSLLFQQTGIGKADAVRLGFEKASGDILMILDADLTVPPEDLPRFYDAIASGKGEF
                     INGVRLVYPMEKEAMRTLNFIGNKLFSMAFSWLLGQPIKDTLCGTKVMWREDYEKIAA
                     NRSYFGDFDPFGDFDLIFGAAKLNRKIIDLPIRYRDRTYGTTNISRWKHGILLFRMVA
                     FAARRIKFV"
BASE COUNT         2137 a         2669 c         2694 g         3100 t
ORIGIN      
        1 cgattcccca aaaaaattcc cgctctggaa cattgccaag atcatccttg cgttgattct
       61 ggtctggttc gttttatcca aaacagatct gacccaactc tacgccctgc gtgaacggat
      121 ctccctgccg tggctcgcgc tgactctcct cctgtatttt tccctgaccc tactcaaggc
      181 cctgcaatat tattttctca ttggccgccg cgtggattat ccacaggtgt tgaatatcgt
      241 cgtggtgcaa aatgccattt cgaatttcat cgccacaagc gcgggcattg catcgtacct
      301 tgctttgttt cgcgtcgagc agggagtcaa ggtcagccgc gctgcgctgg cttttctcct
      361 cgccaaagtt ggagatttga tctccatctg gctgttcatg ttggtttcca gtttgctcgt
      421 ctggccgttg gtggcttcat ttcacagcct tattcttctt tccctgtctt ttattggtgt
      481 ggcaattctt gttttttttg cagcagtttt tctgcgccag aaatttgttt ccgttctggc
      541 ctggtttgtg gaaaaactca aactgtcgcg gctcggcttc gtcaataaac tcatggatct
      601 gcttgccagc ctcgccgaac aggagcatgg atttgttttt cgcatggttg gcacgggcat
      661 cgctttctca ctgatctaca tgaccgtcac catgctgtgg ttgtacgcca gtttgcaaac
      721 cttttccttc gaaattcagt ttttacaagt tgtctttgtt aatatcttca tgcaattgat
      781 ttcctacctg cccattcaag tctttggcgg attgggtgtc aacgaaacca catcgctgta
      841 tctctatggt attttcaact ttccgcagac ggaacttgcc gcgaccctga tcggcacacg
      901 cctgcttttc tatctgacaa accttgtggt gctgttatac ttgcccgtac atactttgtt
      961 ttttagccgc tctgcgcaaa aacaatagca tatcttcaat cgtttgtccg tttctcgatc
     1021 cgctttatgc aaaatgcctc ccctaaaaac atcattcaat tactcactgt aggtctggtt
     1081 tcaggcctcg tttttgcttt cctcgaaatc aaggctctgt gggtccctga tcaaatttgg
     1141 aataaattac tcacgccgct cggcgtagcc gcaatcattg cctannnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnccgaac aggatcagcc cttcggctgg agtgaccttg
     1321 cactggcgtt cagcattttt ctgtatccgc gcatcgtgca ggaaatacgt caatatcttc
     1381 ctgcgccgct gctcacccgt ggatcgattg cggtgggatt tgtctttcaa tttgctttgc
     1441 ttgccgcttt gtatgaatca gtagggaagt ccctgctcgc aaaattgatc gagcttcgag
     1501 cccgcctcga acctgttcgt ctaattttgg ctgctttgtt tttgctggct ccatttattt
     1561 atcgttattc cactggcccg aaagaataca ttttatattt caatatccgc ttcatggttg
     1621 ccttgatcgc tttatggttt gccgcatatc tgctttgcaa agactcaagc cgactcattt
     1681 ccccagagtc ttttggcgtt gcggctggct ggctggtgct tgtctctgcc attacgcgct
     1741 cactcctgct tgttgtggat gatcccttcg gcctgtactg gtctgaaggc aaccgcttgt
     1801 acgattattc ccttgttttc gggcaagatt tatataacta cgcggggcga attcccgatc
     1861 cctacaacac gcccggacgt tacggtttgt ggggcgtgct ctacctctgg cagggacttc
     1921 ccatctgggc gcatcgtttg tggaatgttg ccttgcttac ggtcccgtct tttgtgctgg
     1981 cgtgggcatt gaccaaaaaa ctgtccccgt cctttttacg gcagatggtt tttctttggg
     2041 ttacggcttt ctttattttg ctcgcgccgt tacatccgcc gttcatgatt atgacggtca
     2101 tcgtggcatt gttcattttc cacccgtcgc catatgtgcg cggtgcttcg cttgtagccg
     2161 ccagccttta cgctggcatc agccgctgga cgtgggtctt cgctcccggt gcatggggtg
     2221 cgttgaccga cctgcttttg ttttacccaa agcgtgaagg caattggttc aagcgtttgc
     2281 ttcccacggt tttgatggcc gtgctcggcg ctgtccctgg gtttttgctg aacatcggaa
     2341 attttcttgg atatacaacg ggagagaata ccaccgcgca gcagccattg ctttggtatc
     2401 gcctccttcc caatctgaca cttggtccgg gtatcgtatt gttgatcctg ctgacgacag
     2461 gtccgctctt ggtgttgctt gcttacaaaa tgtccacacg tcagtggaaa ttggatggat
     2521 ttcagttgct ggctgtgtgg ggggcgttga tcggtttctt cacgattggc ttggtgatca
     2581 gcaccaagat cggcggcggc ggtgacttgc acaacctcga catgtacatt ggcactctca
     2641 tcgtggtgac catgcttggg ttgactgggc aggttgaggg tcaggatgtg aagcgctggt
     2701 cttcatccgc attgacgatg gtatgcctgc ttgccattct gccgatctat ccattcacac
     2761 catttcatcc atcggctggc tcgcatgcct ggctcgacct gcccacccgc gaagataccg
     2821 acaaggcgtt gaaggtcatc aacgattcgg tggcgaatta ttcgcaggcg ggcgaaatcc
     2881 ttttcatgga tcaacgtcag ttgttgacct ttggctacgt gaccgcgatc ccgtttgtgc
     2941 ctgagtatga aaagaaatat atgatggatc aggcgctggc ttcgaatgag acatacttcc
     3001 gcccgtatta tcaggatctt gcaaataaac gctttgcgtt gatcgtaacc gagccgctca
     3061 aagtggatct gaaggaagag ggcggtgtgt tctctgagga aaatgacctg tgggtcaagt
     3121 gggtttccgc gccgaccctg tgtttttacg agcaagtcca tgccgataag acggtgggga
     3181 ttgcgctgct tgtgccgcgt gagaatcctg ttggctgtga aaaatatttg aaataataaa
     3241 atcggtggtt gagcatgccc aaacccctgc aagggccacc actaaaaagg tactctcttg
     3301 aatttatctt atcttcttcc tcgtatactt cgccacttca tgcctgagtc ggtggcgcgc
     3361 tttttattga aacgccgctg gatcatccgc cctggattgg aatcgagtga cccgttcgcc
     3421 gcggttgagc agtacatggc tgggataacc gctagcggaa aatccctgat cggaaaacgg
     3481 gtgctcgtct ttggttatgg cggtcgtttc gccgtgggtg tggaattgct gcggcgcggt
     3541 gcggcgcatg tggtgttgtg cgatcatttt gtcctgctcg acaaggagcg taacctggag
     3601 ttgctccctg tttaccctga gtatcttgtg ggcgaaaatg ataatgtgcg cccgcgtgcg
     3661 gaatatatca ccctgttgca tggggatatt cgcgaggatg caattcagaa gcagatcacg
     3721 gaagtggatg ttgtcctcag tacatcggtc tacgagcatt tggatgatgt caaaggcatc
     3781 actcatgccc tcgcgaaatt gaccgcatcc aatggattcc acctgcattt tgtagacctg
     3841 cgcgatcact ttttcaaata tccctttgag atgcttactt tttccgaagg tgtgtggcgg
     3901 aatttcctca acccgacgag caacctgaat cggtttcgct tgggcaatta tcgggaagtg
     3961 tttgatgcgt attttcgaaa agtggaaatc aagatattgg aacgtctcga acctgaattt
     4021 cgtgccatcc gtgcgcgcat ccgccctgag ttcctgacgg gtgatgaggc ggttgattct
     4081 gtcggcttga tccaggtcat cgctgagatg agaaagtaaa gccgacttaa gtcggcgctg
     4141 tgccagttgg agattttttt atcatgcaac aaagattttc cagttttata atccgaaacg
     4201 aaaagctcat tcccgttttt atctttctgc tttttcttgc ggcatccctt ccaggtgtgg
     4261 attggggtgt gcccgcgctt tggaatcccg atgaaatgat ctggcgcgtg gatagcgcct
     4321 tgcgcggcga gatcatcttc gatgagaccg aacccgattt caattacccg tcgctcccca
     4381 aatatgtgat gttcggcatc ggcaagatcg tgtatgggtt gggcggcacg ccctcagatt
     4441 tttacatttc ggcacgttcg ttctcggctt tattgggagc ggtggctggt gtgcttgttt
     4501 atttcatggc acgcagcatc ggtgtggatg tgttgacctc tgcgttggct ggcttgctgt
     4561 atgttgccag cggtgtagcc gcagcaaatg gacgctttgc acacaatgac ctgtatttgc
     4621 agttgttttc cgtcctctgc gtttatttcg tgatcaaata tcaatttaca aaagcgcggc
     4681 tgtggttgta tggttcattc ttttcggtgg ggctggcggc ttcgagtaaa tttacgggcg
     4741 gcagtttgct gcttgtgcca ttgttcgttt ttgttttcat gaactggagc gaggttcgca
     4801 ggaactggct gcaatcgttc gagatgcttt tcattggcgg cgcactcagc ttcggcggat
     4861 acatccttgg cacgcccaag gccttgctct ggatggcgta ttacttcaag cgcgtcattc
     4921 ccgcgttgca gcgttatccg caatatggtt tcaactcagg cacgcccatc ggcttgtttg
     4981 gtcaatggcc ggtgttcgaa gatgcagtgg ggaccttcgc ttactatatt tttattcttt
     5041 gttttatctg gtttgtagcc aaaataattc tttggaaact cggcaggctg caaatggatg
     5101 aaacacaggc acaggcggtc atgatccttg ccgccacggt cctgctgttc gacctgccgt
     5161 tcatgatctc gatcaattac atcccgcgct atttcatccc gtttgtgccg ttcctctcca
     5221 ttttgggcgc gcttttcatc aaagagatcg ttgaaattgc aaaagccaaa aactggaaat
     5281 atgttccgca gatcgtttcc gttttgctgg ttgtgggcat tacctattcc atgctgcggc
     5341 tggcaagcac ggccctgctt ttcatgaacg atgcgcgcat gcccgccagc gaattcgtcg
     5401 caaacctgcc tggcgaaggc aagaccatcg aatacacctt gtacccgccc attgtgaaca
     5461 aaaagcaatt tgcaaaagcc tataactatc ccgtcttttt tttgaaatac ccgactgacg
     5521 ttgtccccac aggcggacga tacgaataca atcaaggcga ggcgggatta atagaccgcg
     5581 aaaccgacta cctgattatt gatacgctta cctatgggcg actctacacc gaatccgtgt
     5641 gcgtgaccaa ccccgtggaa tgtgatttct tcaagcgtct ggttgcgggc gaaataaaga
     5701 ccttccgcct gatgaaggaa tttacctaca ccctcccgcc ctggctgcca gatatctctc
     5761 tttcagcagt caaccccgag gtccgcattt atgagcgggt gcgttaaaaa gagacagtca
     5821 ccttcgtgta gcgggtgact gtctcttttt tttttatttc acaaaattcc ccaccgtata
     5881 cacagaccct gcaatcggcg gcgtgctctg catgtggatg acatgtccct ttgaaaacgc
     5941 cttgtgcgcg gcctcttcga tttccttctc ggtcgaaaaa tgttcatcgc ggatattatg
     6001 cccgagcaag ctgaacatga acaccatgaa cggtttttca acgggagttc catagaccat
     6061 ttgcccgcca ggtttcagta cgcggttcac ttccgcgaag gcctgcttca attcggcagg
     6121 tttgagatgc tccaaaatgc tgaccagcaa aaccgtgtcg aagaagttgt cctgatacgg
     6181 catcttcaac acgtcgccct tttccagaaa cagcggaatt cccgtcggct caaaaacaga
     6241 cttgacggca tggatgtctg cggtcagatc aatgccatgg atctccttgt acatgtcgtg
     6301 caggttgggg aaagccagtc ccgtgccgaa gcctacttcc aacacgcgct cgccgcccgt
     6361 gcattcaccc aatgccaatt ccacgcgttg acggtacatc ttcccgaaaa cgggccagta
     6421 atagtatttg atcgggtcgt actgattgac gcctttgtat tgtgaggcag ggagtagttt
     6481 gagtttcggg ggcatgcttt ttcctgtttt ttgttttaag tcccgtgatt ttacctgatt
     6541 aaacaaacta cggcttccca tctctcggcg ttgttttgct ctttgaggat atgcgccaat
     6601 ggcgtataat ggttttgatg attgttgtag aaacacatat tttcacgcgg caagttttgt
     6661 tgcttttgag cgatgaagag taccgtttat tgcaggcagt acttgtcaat cgtccggagt
     6721 ctggtgtgat aatcccttcg agtggcggat tacgaaagat gcgctgggga tttttaggga
     6781 agggcaagcg cggcggtgta cgtgtgatct actattgggc agtcgggcag gaacgtattt
     6841 tgatgctttt gatttacccc aaaaacgtga aggatgatct cacgcgggaa caattgaaaa
     6901 ttctgcggaa cattgtcaaa gaggaattcc catgaaagaa gatttgttta atgagctggt
     6961 ggtaagtgtg cgcgagggtg gcgcaatttt gcgcggagaa aaaaaacctg cacgtgcatt
     7021 taaaataact gccccaaata tcaagcgcat tcgggcaggt tataaacttt cccaacagga
     7081 atttgccgct ctactgggga ttagtgttgc caccttgcgg aattgggaac agggcaggcg
     7141 gatgcctgaa ggccctgcac gggttttgct tcaggttgcc gcacgctatc ctgatgtggt
     7201 ttgggatgtg gtgaaaccta tccgatagcc aatctcattt atttggattt tgttgttatg
     7261 cccttttcct cttctaaaaa atggagcgcc ctcttcgctg tgtggattct gcatggcatt
     7321 gttgccctgt ggcaattttt gtccctgcca tcggaggggt tttcgcttca acgcattgta
     7381 atgcttgggc tgctgctggc ctgggttgcc ttcgccacgt tcctggcttt ctcggctttt
     7441 cgccagttcc actggttgaa tggacttcta ctccgaatca ataaaccatt cataagagat
     7501 ggtattttta tcgctgctgt tctggctttg tttgtccgca tcagcctgat gtttttgctc
     7561 gggctggccg cccagactcc agagtttcat tacggcgcct atgttgaacg cctgtcgccg
     7621 tttttagacc tgctggcttt tgtcgccttt gagatcatcg tgctgaattt gttctttgtc
     7681 tttcgggagg ggacagaata tttcggcttg atcaagtcca tttcggcgaa actgttcatc
     7741 ggtctggttt tgatcggggg tgtggtcctt tacatttcga aaaccgacat gggcatcgct
     7801 cccatttaca aaggcgactg ggcacgtgga cttcccgccg tgcccttgct tgaatggcag
     7861 atttttcttg ccgtgttgtt ttgcatcggt atgttcctcg ccgaagtgga tggacgcctg
     7921 ctgaaaattc ccagcctcga tctgtggatt gctcttgcgg tttgggttgc cacggtcgga
     7981 ttgtggctga gccagcccac tgtgccgaat gcctcggctc tcgaaccgcg cgaaccgaat
     8041 tacgaaatct atccattcat tgacgcgcac acctacgaca aattcgccca atccgttttg
     8101 atcggcaatg ggtttggtcc caatgaaatt ccgcagcgcc cgctgtatat tgtttttctg
     8161 gttttcatgc acacggttgt cgggcagaat tacgaaaatg tcattgcact ccagaccgtg
     8221 ttctttgcag cgttccctgt tgtattgtat atttttggca gggaattttt tgggcggccc
     8281 atcggcgttt cgctggcttt gctggctgcc ttgcgcgatt tcacctcaaa tctggtttcg
     8341 cccatcacgg ggaatatcag ttattcaaaa ctgtatctct ccgaaatccc gactgcgatc
     8401 tgtctgatcc tgttcttgct catcggcatc cgttggatca gatcgggttt cccgctttat
     8461 ctcggttttc tgcttggcgg tgtattgggt attggcatgt tgaatcgtac gcaagtggtt
     8521 gttgctctgc ctgttttgct tttctttgcg ttcattcttc agccgaaaaa acttctcttc
     8581 atcattaagg gcacgcttcc aatgttggca gtgatcgcgc tggtcgttgc cccgtggctg
     8641 tggcgcaact ggcagatgac gggcaagatc atcttcgata atcccgcctc gcaaaccgcc
     8701 aacctcgccc tgcggtacaa ccgcctcaac ggcgaagatg tggatattct gcaaaagcct
     8761 ggcgaatcta attctgatta caacgaccgc atggttgcaa tggcgaatca tgccatgtcg
     8821 gtgaactcgc agggcatttt caaggcgatc gcaagttcgt ttatcaatca cggcatcaac
     8881 aacattttgc ttttcccgct ccgcaacgac ctcgcaactc ccgctgaact cattacccca
     8941 accacgccat tctggcagca atgggaaggc cgtcccaact tcctgcaatc gctcttgctg
     9001 gctttttacg tttttctttt ctcgctcggt gtcaccatgg cctgccagcg caacggctgg
     9061 cttggccttc tgccgctcat ggtcaacctc gcttacaacc tgtggacctc catcgccctg
     9121 ctttctggtc aacgcttcat gctttcgatg gattggtcca ttgccatgta tgatctgctg
     9181 ggcattttcg cattgctcag tatctttttc tatgccctcg aaaacggacg cgcgaaaatt
     9241 ctcaaatggt atgaagccaa cacggtttcc atcattccaa ccctcgaagc tataaaatgg
     9301 ccgcgctatg tttttgcagg cctgatgttt ttcgcggtgg gtgcatccct gcctttgagt
     9361 gagatgtctt tctcacagag atacccagcc cttgagcaga atcaacccgc tgacctgact
     9421 ctcctctcaa gcctcgctca aaccgacctc gaacctgcct gccaaaaact ggcagaatcc
     9481 agcgggttgg agatcgtgcg cgggcgggcg ttgtatccgc gtttttattc agcaggtgac
     9541 ggtgagatct tcactgattc ggttggctac aaaaaagtgg atgaaggcag gttggtgttt
     9601 gacctgctcg gtcagaggaa tcaccgtgtc atcttcccca tgagccaatc gcctgaattt
     9661 tttcccaatg cctccgatgt gaccatctgg ttcgataaaa acgcatctcc ctggttcatt
     9721 ttggttgaac agggagatgc gcaaaggttt tatgtttcag atagggtgtg taagtaatct
     9781 tttggtagcg tggactttag tccgcatttc caaagcacag actgaagtct gcgttacgcg
     9841 agttacacaa acttaattcg tctcgccgca aaggccacca ttctaaacaa cagaatgccg
     9901 tgcttccagc gcgagatgtt ggtcgtgccg taggtgcggt cacgatagcg gatggggaga
     9961 tcgatgatct tgcggttgag tttggctgcg ccaaagatca agtcaaaatc gccaaaggga
    10021 tcgaaatcgc caaagtagga acggttggcg gcgatctttt cgtaatcttc acgccacatc
    10081 acctttgtgc cgcatagtgt gtctttgatc ggttgtccca acagccacga gaatgccatg
    10141 ctgaataatt tgttgccgat gaagttcagc gtgcgcatgg cttccttttc cattggatac
    10201 accagacgaa ccccgttgat gaattcgccc ttgcccgatg cgatggcgtc atagaatcgc
    10261 ggcaggtctt cgggcgggac ggtcaagtct gcgtcgagga tcatgaggat gtcgccggag
    10321 gctttctcga agccgaggcg cactgcgtcg gctttgccaa tgcctgtttg ttgaaagagc
    10381 aggctcgggg ttgaagggtg aagcggaatt tcatcctgaa ttgtctggaa ggtgttatcg
    10441 cgcgagtgtc cttcgacgaa gatcaattca gtctttgatc ccatcttggg cagacgctcg
    10501 aaaatattct tgatattgcc tgcttcgttg cgcgcggcga cgatcacgga tacgctcgga
    10561 ttctttgcag gtgttgcagc gggtcgcgcg attacaaaat tggacagcgc aaaatcacgg
    10621 aagggccaca gcttgacgag gtaacgatcc gcgaggccgc cgaggggcag aggccagagc
    10681 acctcgcgca tgggacggat
//
LOCUS       DAOQJC010000132        10668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_3206_length_10668_cov_9.816734, whole genome shotgun sequence.
ACCESSION   DAOQJC010000132 DAOQJC010000000
VERSION     DAOQJC010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10668
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3206_length_10668_cov_9.816734"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLE39_15910"
     CDS             complement(<1..446)
                     /locus_tag="PLE39_15910"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNE69868.1"
                     /translation="MTEITVGELLAKCLQAEGIEMMFGIIDGAHIPFTVHAPKYGIRH
                     INCRHEEGAVHLAEGYTRTNGKPSVVIGSPGPGGANMLAGVSSAYAEGHPIIAIACTR
                     RRLTTDPERGGAWQATDLVEMAKPITKYSALVRQPERLPEMMRAAFR"
     gene            complement(551..1531)
                     /locus_tag="PLE39_15915"
     CDS             complement(551..1531)
                     /locus_tag="PLE39_15915"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE69869.1"
                     /translation="MNQTRYQKVFITGALGFVGRAIAAHYRTLGAQTCGLDVRADAGL
                     NVVAGDVSRPEEWRDAMQGCDLVIHTAAIVTNNVTRAEAWRVNVLGTRCVLDAARQAG
                     VKRFVHVSSLAAMRFNEGDHAADESAPVMPTGNPYVDTKIASEHVVLATHAKGEIPCT
                     VIRPADVYGPNSRPWTLIPVQMIQKGLFLLPANGQGIFRAIFIDDLVNGIVLAAEKEE
                     AIGQIFILGGEVATTCETFFGYYYRMLGKGKPRTMSTSTAVAIAEAGRMIFKLLGKPT
                     ELGRGAMEMLSKKSTVSNQKAHDVLGWYPQVDLEEGMRRTEVWLREQKIL"
     gene            complement(1528..2346)
                     /locus_tag="PLE39_15920"
     CDS             complement(1528..2346)
                     /locus_tag="PLE39_15920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE69870.1"
                     /translation="MTSKTAIITGAASGIGKHWADVLLKQGGYQLVLADVNEQGLRGS
                     FSESASLRLHALDIRSKEQWQRVLDDALQRFGRVDYLFNIAGISRQSFFLSQPIENID
                     LVLDINLKGPLIGMRLVGEQMMKQGAGHIINVGSLAGLSPTPGNGLYSAAKAGLRNAS
                     IAAAIEWRTKGIFVTVIAPDLVDTPIMQRHLESAKEEVALTYTGVTLTVHDIEKAFWK
                     AMREKPLEINLPRWRGWLTKLNHANPAMMFWLYEPMKRRGMKRFENIRKQRLGK"
     gene            complement(2347..3738)
                     /locus_tag="PLE39_15925"
     CDS             complement(2347..3738)
                     /locus_tag="PLE39_15925"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE69871.1"
                     /translation="MIYLRKFHISEEDGTMANVAPNSVSTRRERVAWYLYDFGNSAYA
                     SVVLLAVYSAYFQKQVVGGAEGSRIWGLTISIAMIIVAIAAPIMGAIADYSGAKKRFL
                     FFFTALSVVFTAMLFFTQKGTVMLAIVCFLLAEIGYRAAQVFYDALLPEIASPGEMGR
                     VAGTGWAIGSAGGIAILLLILPPILMTKSDLLVVRGSLIATAVFFAAAAAPIFLRLTE
                     RAKPQQLPPGENYFSVAFKQLGETIKAARGFNEFLKFMLAYLVYNEGVIIALDFAAIL
                     GAVLFGLEQTGLIIFFILVQATNVLGALLFGNLQDRFGGKPSLSISIMLMVACIAALY
                     FAQNQTHFFIIGGFVGVAMAGVQSVSRAMVATFSPPGKSAEFFGFFALTGRTSSFIGP
                     AVFGWLAAELTLWYQAQGQDLALAEQSGHRLAILSIAAFLIAGWALMLMVNEKKALET
                     VGQMTVEDTTASK"
     gene            3847..4788
                     /locus_tag="PLE39_15930"
     CDS             3847..4788
                     /locus_tag="PLE39_15930"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNE69872.1"
                     /translation="MENPTSDEKILAALSHAAVIFPMLGPVVPAGIWAFQRQKSKYVR
                     FHAMQAMGYQTYGLWLWFVGMFLSIFLLMIAVIPLEIALSDNPDTLAMSPLIIQPVFF
                     LIILGSWGLFFLGGAIGAVFCLLNREFRYPIIGNWLKQKLFHDQVTDAEFEKWEENWI
                     SGVCHATVILRFWGIITPFMLWILEREHSVKIRFQALQAALYQLLYVVAAIASTAIGV
                     VFYVLFIIVMAIGLNGTSPAANDEIPAWLGAVLIGVMVILILYAIATFIIMPVYYLMA
                     LIGSFRVASGHEFYYPILGDIIAKRMKPVQVEVEPQP"
     gene            4785..5249
                     /locus_tag="PLE39_15935"
     CDS             4785..5249
                     /locus_tag="PLE39_15935"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNE69873.1"
                     /translation="MNDTSISTVSSDEKLMGAAAHFFGPLAALIIWGTQKDKSPFIKF
                     HTLQALAFDLVTITSLGIFFFCAFGVVFVGMFGSIFSMTYTSSPESEFRFLLPAVLFP
                     VMITTCITPISLLITAVRLAASFSILSGRDFRYPLIGKWLENFLKNDINSSP"
     gene            complement(5306..5653)
                     /locus_tag="PLE39_15940"
     CDS             complement(5306..5653)
                     /locus_tag="PLE39_15940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE69874.1"
                     /translation="MEIHYRDLKDSMGLVHLIGSLDISGVGQIETRFAGYCAGEQPRV
                     IVDLSEVDFMSSIGIRLLTLNAKSVATRGGKMVLLNPTPDVLSVLEITGIPAIIPIYS
                     HIESAETVLRSSR"
     gene            complement(5762..6295)
                     /locus_tag="PLE39_15945"
     CDS             complement(5762..6295)
                     /locus_tag="PLE39_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69875.1"
                     /translation="MFKNLFGNQQSEPQMVMSEGYKNWRGMIFLVTPGQIELNIKEPN
                     KVFGVILDVGVKDMNSNSAFVFTTAVFANGDATFFPSPGGGVIGLGNNPQVAATSKSI
                     VTMGQIFLARAQPAKENSTASVSRVKFYFLTTSGLFVTEDSLKAFQSGPYGQLLGKFG
                     QIRAVAEQMIDKRLGKK"
     gene            complement(6323..6664)
                     /locus_tag="PLE39_15950"
     CDS             complement(6323..6664)
                     /locus_tag="PLE39_15950"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE69876.1"
                     /translation="MKLQSSDLENGIRLIKLVGKLDSNGTYAIEIEFIKSCAGEKPRI
                     LVDLSKVNYISSIGIPMLVNTARSVMARGGKLGLLNPQRNVLDVLELVGVSMMLPIYY
                     DLKTATAGMLA"
     gene            complement(6794..7720)
                     /locus_tag="PLE39_15955"
     CDS             complement(6794..7720)
                     /locus_tag="PLE39_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69877.1"
                     /translation="MKSPRFSTLTLYFLSLALFVTACVGGEATATVLPVATEFIPPLD
                     EPTPIPFTGHTPFPTPSSLPAVNQASTSPEWVADFSDPILAKLVNRKPDFQDDFKLVC
                     IYNSQWLDNCPTEDEMPNFIDDLSVLNRGWFYRIPGSTSGPFYSDLKYETLFLNSPKG
                     KSDRDLMVYNPYLLHENFVLSFDFQFKETQPDDFVRFQFNQSKDQSVNLDLWKNKSWS
                     FYGPLQEVSGAFDYFPPELINITIIMLGRECAAYLNDVPFAYLNDCRSEAVVGSFPWA
                     VSFHVIAAPGHVARAAIDNVKLWDLDKIKELQ"
     gene            7816..8592
                     /locus_tag="PLE39_15960"
     CDS             7816..8592
                     /locus_tag="PLE39_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69878.1"
                     /translation="MHTPTSATHQFKYQPEKNRRGIMYQYTKSNVDGSAPARVYIRMA
                     DEYVIDVWKFESHNFDAAHVQARMNWDFFSAKQLDSWVVTSDGASRPQARMNVSFEEA
                     VVTVQIQHMEGKTEIGHFPFHIYNFDFISLNMSLPHWVNPESELSIGVVQPNFNPEIR
                     SLLNYEGIATLKFIGNEKRNGTPCRKYSLEGQWLNGQAGQLWVSQTEGHIEDMEIPIP
                     DNPDWDDFKFNLVSTQSMDDAQWEQFISTEIKKLTPVDGE"
     gene            complement(8655..9101)
                     /locus_tag="PLE39_15965"
     CDS             complement(8655..9101)
                     /locus_tag="PLE39_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69879.1"
                     /translation="MNYRLMFIINAIASALLGAVLLLMPEFVLVQLGAETYVSTQLMV
                     RFFGGTLLVSGVLLWFLKDVAAKVQKGAGFALLGGSVGGFALSIIGMTSAGIFRSNGW
                     VLLVIFGVFSLVYGYLLFLQPKQSDAKARAPRKPKEAPSAGGGQSV"
     gene            9269..10180
                     /locus_tag="PLE39_15970"
     CDS             9269..10180
                     /locus_tag="PLE39_15970"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69880.1"
                     /translation="MTNFTIRKGKQTDQFAVFCVFRLSSYDLLQKMKVVTSIPTLAEI
                     ENLYEYYRSFLAHIYDSCDQFWVAEADGQVVGYARSIVRDGVRQLTEFFVMPDHQGLG
                     AGSQLLAKAFPKEGTQNRVICASPDTRALIRYLKTGVRSRTTIYEWSRIPEAVPFETD
                     LVIKPIENTPANIAILNEIDHTILGYTRETDHHWLIQNKRGHFYLRQNQVVGYSYFAN
                     RAGPIAMLNNADFSAALAHIETEMTATVDEVYNEIILCVPMSNPAAVDYVIRRGYHTN
                     AFFEHFLTDKPLGHYENYIFIDPILTT"
     gene            10436..>10668
                     /locus_tag="PLE39_15975"
     CDS             10436..>10668
                     /locus_tag="PLE39_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNE69881.1"
                     /translation="MYRSNLCGELRASHAGQTVTLSGWVNRRRDHGGVAFFDLRDRAG
                     IVQVTINPDLPKEVLDQVASVRNEWVLQIEGVV"
BASE COUNT         2808 a         2904 c         2444 g         2512 t
ORIGIN      
        1 cggaaggcgg cacgcatcat ttcgggcagg cgttcgggtt gacgcaccag cgcgctgtac
       61 ttggtgatgg gtttcgccat ctccacaaga tcagttgcct gccacgcgcc accccgctcc
      121 gggtcagtgg tgagtcggcg gcgggtacag gcaatcgcaa tgatgggatg tccctccgcg
      181 taggcgctgg aaacgcccgc cagcatattc gcgccgcccg gtcccgggct gccgatgacc
      241 acgctcggct tgccgttcgt gcgcgtgtat ccctccgcca aatggacagc gccttcttca
      301 tgcctgcaat tgatgtgccg aatgccatac ttcggcgcat ggaccgtgaa cggaatatgc
      361 gccccgtcaa tgatgccgaa catcatttcg atgccctctg cctgcaaaca ctttgcgagt
      421 aattcaccaa ctgttatttc agtcataagt tcctcctcaa gttttttcaa ccacaaaggg
      481 tcacaaaggt acacaaaggt cttttgaggg tttcctttgt gaccctttgt ctcctttgtg
      541 gtaaatgcat ttacaaaatc ttctgctctc tcaaccaaac ttccgtcctg cgcatcccct
      601 cctccaaatc cacttgcgga taccagccta aaacgtcatg cgccttttgg ttggataccg
      661 tgctcttctt cgagagcatt tccatcgcgc cgcgccccaa ctcggtgggc ttgccaagca
      721 gtttgaagat catacgcccc gcttccgcga tcgccaccgc cgtggaggtg ctcatcgtgc
      781 gcggttttcc cttgcccaac atgcggtagt aatagccaaa gaaagtttcg caagtggtcg
      841 ccacttctcc gccgaggatg aagatctgcc cgatggcttc ctctttttcc gctgcgagca
      901 caatgccatt cacaaggtca tcaatgaaga tcgcgcggaa aattccctgc ccgttggcag
      961 gcagaaggaa caatcccttt tggatcatct gcacaggaat caaagtccac gggcgggagt
     1021 tgggaccata cacatcggca gggcgaatca cagtacatgg aatttcacct ttggcatgag
     1081 tcgcaaggac aacatgctcg ctggcgattt ttgtatccac atacgggttg cccgtcggca
     1141 tcacaggcgc ggattcgtca gcggcatggt cgccttcatt gaatcgcatc gcagccaatg
     1201 atgagacatg cacgaaacgt ttcacccccg cctgcctcgc cgcatccagc acacagcgcg
     1261 tgcctagcac attcacccgc catgcttctg ccctggtcac attgttggtc acgatggcgg
     1321 cggtgtgaat gaccaggtca cagccttgca ttgcatcccg ccattcttca gggcgggaga
     1381 catcccctgc aacgacattc aaccctgcat cagcacggac atcgagtccg caggtctgcg
     1441 cgccgagagt gcggtaatgc gcggcaatgg cacgccccac aaagcccaac gcacctgtga
     1501 tgaaaacttt ttgatagcgg gtttgattca ttttccgagt ctctgcttgc ggatgttttc
     1561 aaaacgcttc ataccgcgac gtttcatcgg ctcatacaac cagaacatca tcgccgggtt
     1621 ggcatgattc aacttcgtca gccagccgcg ccagcgtggc aggttgatct ccagcggttt
     1681 ctcgcgcatg gctttccaaa aggctttttc aatatcgtga accgtgagcg tgacgcccgt
     1741 gtaggtcagc gccacttcct cttttgcgct ttcgagatgt ctctgcatga tgggcgtatc
     1801 gaccagatca ggcgcgatca cagtgacgaa aatgcccttc gtccgccact caattgcggc
     1861 ggcgatcgag gcgtttctta atcctgcttt ggcggctgag tacaatccat tccccggtgt
     1921 gggggaaagc cccgccaacg atccgacatt gatgatatga cccgcaccct gtttcatcat
     1981 ctgctcgccc accaaccgca tgccaatgag cggacctttc aagttgatgt cgagtacgag
     2041 gtcaatgttt tcgatgggtt gtgaaaggaa aaaggattgg cggctgatgc ccgcaatgtt
     2101 gaagagataa tcaacccgcc cgaagcgttg cagcgcatca tccagcacac gctgccattg
     2161 ctccttcgag cggatatcca acgcatgcaa gcgcagggac gcgctttcgg aaaacgatcc
     2221 gcgcaatccc tgctcattca catccgccag cacaagttgg tagccgccct gtttcagcag
     2281 gacgtctgcc caatgtttgc cgatgccgct cgcggcgccg gtgatgatcg cagttttaga
     2341 ggtcatttat tttgaagcgg tcgtatcttc caccgtcatt tgcccaaccg tctccaaagc
     2401 cttcttctca ttcaccatca acatgagcgc ccaacctgcg atcaaaaatg cagcaatgga
     2461 gaggatggcc aagcgatgac cggattgctc cgcaagggca agatcctgcc cttgagcctg
     2521 ataccacaac gtcaattccg cagccaacca cccgaaaact gccgggccaa taaaggaaga
     2581 cgtccgcccc gtgagcgcga aaaaaccgaa aaattcagcg ctctttccag gcggactaaa
     2641 cgtagccacc attgcacggc tgaccgattg cacacccgcc atcgcgaccc ccacaaatcc
     2701 accgatgata aagaagtggg tctggttctg tgcaaagtac aaggcggcaa tgcaggccac
     2761 catcaacatg atcgagatcg aaagtgaagg cttgccgccg aaacggtcct gtaaatttcc
     2821 aaagagcagc gcacccagta cattggtcgc ttgcacaagg atgaagaaaa tgatcaaccc
     2881 ggtctgctcc agaccaaaaa gcaccgcacc aagaatggcc gcaaaatcga gcgcaatgat
     2941 cacgccttcg ttgtaaacga ggtacgcaag catgaatttg aggaattcgt tgaatccgcg
     3001 cgcagccttg atggtttcac ccaattgctt gaaagccaca ctgaaataat tttcaccggg
     3061 cggcaattgc tggggcttgg cacgctcggt caggcgcaga aaaatagggg cagccgccgc
     3121 tgcaaaaaag accgctgtgg cgatcagaga accacgcacg accaacaggt cgctcttggt
     3181 cattaatatg ggaggaagaa tcaaaagcag aatggcaatg ccacctgccg agccaatggc
     3241 ccaaccagtt cctgccacac gtcccatctc tccgggagaa gcgatctcgg gcagaagcgc
     3301 atcataaaag acctgcgctg cacgatatcc gatctctgcc agcaaaaagc atacgatcgc
     3361 cagcatcacc gtgccttttt gagtaaagaa aagcatggcc gtaaaaacca ctgaaagagc
     3421 cgtgaagaag aacagaaaac gtttctttgc gcccgaataa tcggcaatgg cccccatgat
     3481 cggcgcagcg atggcgacaa tgatcatcgc aatcgaaatg gtcaaccccc agatccgcga
     3541 tccctcggcc ccgcccacca cctgcttttg aaaataagca gagtagaccg caagcaacac
     3601 caccgaagca tatgcggaat tcccaaaatc gtacaggtac catgccacac gttcacgacg
     3661 tgtggataca gaatttggtg cgacatttgc catcgtcccg tcctcctcac tgatatggaa
     3721 tttgcgcaag tatatcacca cgaaaagaac cgccgtgggg ttgggtaaaa aaatcttcaa
     3781 atattgaata tattcatcaa atgggagtat gattgcgaca tccaaagcac cataggagga
     3841 aatcaaatgg aaaacccaac ttctgacgaa aaaatcctgg cagcgttatc tcatgcagcg
     3901 gttattttcc cgatgctcgg ccctgttgtg cctgccggta tttgggcatt tcaacgtcaa
     3961 aaatcgaagt atgtccgctt tcatgccatg caggccatgg gctatcaaac ctacggcctg
     4021 tggttgtggt ttgtgggaat gttcctgtca atttttttac tcatgatagc ggtcatcccg
     4081 cttgaaattg cactgtcaga taaccccgac acgcttgcaa tgagtccgtt aataattcag
     4141 cctgtctttt ttctgatcat tctcggatcg tggggactct ttttccttgg tggggccatt
     4201 ggcgcagttt tttgcttgct caaccgtgag tttcgttacc ccatcatcgg taactggcta
     4261 aagcagaagt tattccacga ccaggtcacc gatgccgaat ttgaaaaatg ggaagagaac
     4321 tggatcagcg gcgtctgcca tgcaaccgtg atcttgaggt tctggggcat catcaccccg
     4381 ttcatgcttt ggatactgga acgagaacac tcggtcaaaa tccgttttca ggcattgcag
     4441 gcagctttat atcaacttct ctatgtggtg gcagccattg catccactgc cataggtgtt
     4501 gtgttctatg tattattcat catcgtgatg gcgattggcc taaacggcac cagcccggcg
     4561 gcaaacgatg aaatccccgc ctggcttggg gcggtcttga tcggcgtcat ggttatcctc
     4621 attctgtacg caatagccac ctttatcatt atgcctgtct attatctaat ggcattgatc
     4681 gggagcttca gagttgccag cggacacgag ttttattacc ccatccttgg cgatatcata
     4741 gccaagagaa tgaaacccgt gcaggttgaa gttgagcccc agccatgaac gacaccagca
     4801 tatccacagt ttcatccgat gaaaaactga tgggcgcagc ggcccacttt ttcgggccgc
     4861 tggctgcatt gatcatctgg ggcactcaaa aagacaagtc tccctttatt aaatttcaca
     4921 ctctgcaggc tctggcattt gaccttgtca ccatcacaag cctggggatt ttcttctttt
     4981 gtgcatttgg agtcgtattc gtgggaatgt tcggcagcat tttttccatg acatacactt
     5041 cctcacccga aagcgaattt cgtttcctgc tccctgctgt tctgtttcca gtcatgatca
     5101 ccacttgcat cacccccatc tctctcctta tcactgcagt gcggctggcc gcatccttct
     5161 ccatcctgag cggaagagat tttcgctacc cactgatcgg aaaatggctt gaaaacttct
     5221 taaaaaacga catcaattca tccccctgaa aatgaaatga aaccgccccg aaggccaaag
     5281 cttcggggcg gtttgaaaaa cgctcttatc tggaagatct caaaaccgtc tcagccgact
     5341 caatgtgaga atagatcggg atgatcgcag gaatgcccgt aatctcaagc actgacagca
     5401 catcgggggt tgggttcaac aataccattt tcccgccgcg agttgccaca gacttggcat
     5461 tcaacgtaag cagacgtatc ccaatggaag acataaaatc cacttcagac agatccacga
     5521 tcacacgcgg ctgctcccct gcacaataac ctgcaaaccg ggtttcgatc tgcccaaccc
     5581 cagaaatatc aaggctgcca attaagtgga ccaaccccat cgaatctttc aaatcacgat
     5641 agtgaatttc catgttttct cctgtggacc gtggttcaag caattatatt aaggtcaaca
     5701 ggattttttg ttaacaacag gttatggttt caaagaatct gcactataaa tgaacaagct
     5761 gttatttttt accgaggcgc ttatcgatca tctgctccgc cacagcacgg atctggccaa
     5821 acttacccaa caactggcca taaggcccgg attggaaagc cttcaaagaa tcttcagtca
     5881 caaacaaacc agaagtggtt agaaaataga atttcacacg gctcacactg gctgtcgaat
     5941 tttccttcgc aggctgggcc ctggccaaga agatctgtcc catcgtcaca atggatttcg
     6001 aggtcgcagc cacctgcggg ttgtttccca acccgatcac tcccccgccc ggagatggaa
     6061 agaaggttgc atcaccattt gcaaaaacgg cagttgtaaa aacaaaagcc gaatttgaat
     6121 tcatgtcctt aacccccaca tccaggatca ccccaaacac cttgttgggc tccttaatgt
     6181 tcaactcgat ctggcccggc gtaaccaaaa aaatcatgcc acgccaattc ttatacccct
     6241 cagacatgac catctgcggt tcagattgtt gattgccaaa taaattcttg aacatacgcc
     6301 tctctccttt ggaaattcgc ggctatgcca gcatcccagc cgtcgccgtt ttaaggtcat
     6361 aatagatcgg cagcatcatc gagaccccca caagttcaag cacatccaac acattgcgct
     6421 gcgggttgag caatcccaat tttccaccgc gcgccatcac cgaccttgcc gtattcacca
     6481 gcatcggaat cccaatggat gaaatatagt tcaccttcga aaggtccacc aaaatccgcg
     6541 gtttctcccc tgcgcagctt ttgataaact cgatctcgat cgcataggtc ccattactgt
     6601 ccaacttccc aacaagcttg atcaaacgga tgccattttc caagtcactg ctttgtagct
     6661 tcatataaaa cctgccttat gattgatata aataatttcg acaaaatgac cgcttcacgc
     6721 ttaaccgaaa attgatccca atctttgaaa cacccaaccc gaaaacattt cgcttcgagt
     6781 ccgcttccat accttattgg agttctttga ttttatccaa atcccacaac ttcacattat
     6841 caattgcagc ccgtgccaca tgccccggcg cagcaatgac atggaacgaa acagcccacg
     6901 gaaacgagcc caccactgcc tccgacctgc aatcattgag ataagcaaat ggcacatcgt
     6961 tgagatacgc tgcacattcc cgtcccagca tgatgatcgt gatattgatc aactccggtg
     7021 gaaaataatc gaacgcacca gaaacctctt gcaatggacc gtaaaaactc cagctcttat
     7081 tcttccatag atccaggttc acactttggt ctttcgactg gttgaactga aaacgcacaa
     7141 aatcatcggg ttgggtctct ttaaattgaa aatcgaaact cagcacgaaa ttttcatgca
     7201 gcaagtacgg attatatacc atcaaatccc tgtcgctttt tcccttgggt gaattcaaaa
     7261 acagggtctc atactttaag tctgaataaa acggcccgct ggtactgcca gggattctgt
     7321 aaaaccagcc tcggttcaaa accgaaaggt catcaataaa attgggcatc tcatcttctg
     7381 tggggcagtt atccagccac tgagagttgt aaatgcagac caatttgaaa tcatcttgaa
     7441 aatcgggctt ccgattgacc agcttcgcaa gaatcggatc ggaaaaatca gccacccatt
     7501 caggagaagt cgatgcctga ttaactgcgg gcaggctgga gggagtcgga aacggggtat
     7561 gccccgtaaa cgggatggga gtgggctcat ccaaaggtgg gatgaattct gtggccacag
     7621 gcaaaacagt ggcagtagct tccccgccaa cacaggcagt gacaaacagg gcaaggctca
     7681 aaaaatataa agttaatgtc gaaaatcgcg ggcttttcat gggatatggt tttcattata
     7741 gtatcccatg ctgatttttg ggaaaccgtt gcatcaggag attataataa aaaagacttt
     7801 attacaggag attccatgca cacccccacc tcagccacgc atcaatttaa atatcaaccc
     7861 gaaaaaaatc gccgcggaat catgtatcaa tataccaaat ccaacgtgga tgggtctgcc
     7921 cctgcgcggg tttatatccg tatggcagat gaatatgtca tcgatgtctg gaaattcgag
     7981 tcgcataatt ttgatgccgc ccatgtgcag gcgcgcatga attgggattt cttctccgcc
     8041 aaacagcttg attcatgggt ggtcacctca gatggcgcca gcaggccgca ggcccgcatg
     8101 aacgtctctt ttgaagaagc ggtagttaca gtgcagatac agcatatgga aggcaaaact
     8161 gaaatcgggc atttcccatt ccatatttac aatttcgatt tcatcagctt aaacatgagc
     8221 ctgcctcact gggtgaaccc cgagagcgaa ttaagtatcg gtgtggttca gccaaacttc
     8281 aatcctgaga tcaggtctct tttaaactat gagggcatcg ccacgttgaa gtttatcggc
     8341 aatgaaaaac ggaatggcac tccctgccgt aaatattccc ttgaaggtca gtggctgaat
     8401 gggcaggctg gccagctttg ggtgagtcag accgagggtc atattgaaga tatggaaatt
     8461 ccaattcccg ataatcccga ttgggacgat ttcaaattca accttgtctc cactcaaagc
     8521 atggatgatg ctcagtggga gcaattcatt tcaaccgaaa tcaaaaaact gacccctgtg
     8581 gatggcgaat aaaaacatgg ccattaaatc aaaaagtcga gactacggtc ccgacttttt
     8641 gatttcaaca aaatttagac ggactggccg ccaccggcag agggggcttc cttgggttta
     8701 cgcggggcgc gggctttcgc atcagattgt ttgggctgca ggaaaagcag gtaaccatac
     8761 accagcgaga atacgccgaa gatgaccagc aacacccagc cgttcgagcg gaaaatacca
     8821 gcagaggtca tgccgataat gctcagagca aaaccgccca ccgaaccacc caaaagggcg
     8881 aaccctgcgc ctttctgaac cttggcggca acgtctttca ggaaccagag caacaccccc
     8941 gaaaccaaca aggtgccgcc gaaaaacctg accatgagct gggtggaaac ataggtttcg
     9001 gcaccaagct gtacaaggac aaactcaggc atgagcagca acacagcgcc caacagagca
     9061 gaggcaatcg cattaatgat aaacatcaaa cggtagttca ttttttctct ccttcatagt
     9121 ggtgttgata aaataaatat acagcatcac caaatgggca ggcaagaaat atcccccgcc
     9181 caaccttaca tttgctttgc atggcccata cctccatatc ccacattttt cctatttgcg
     9241 atttttacta gccacacaag gagccgcaat gaccaacttc acaattcgaa aagggaaaca
     9301 aaccgatcaa ttcgcagttt tttgcgtatt tcgcctgtct tcgtacgacc tgcttcaaaa
     9361 aatgaaagta gtgacaagca tccctactct ggcggaaatc gaaaatttat atgaatacta
     9421 tcgaagtttc ctggctcaca tttacgattc ttgtgaccag ttttgggtgg ccgaagcgga
     9481 tggacaagtc gtcgggtatg cgcgatccat tgttcgggat ggcgtccgcc aattgacgga
     9541 attttttgtc atgcccgatc atcagggact gggagctggc agccagttat tggcaaaagc
     9601 cttcccgaaa gagggtacac aaaaccgtgt catctgcgcc agccccgata cccgcgccct
     9661 gatccgctac ctgaaaacgg gagtgcgctc acggaccacc atttatgaat ggagccgtat
     9721 ccctgaagcg gttccgtttg aaaccgatct ggtcatcaag cccattgaaa acacacctgc
     9781 caacattgcc atactcaatg agattgacca caccatcctc ggttacaccc gcgaaaccga
     9841 ccatcactgg ctcatccaaa acaagcgtgg acatttctac ctgcgacaaa atcaagtggt
     9901 tgggtacagt tatttcgcaa atcgggcagg tcccatcgcc atgctgaaca atgcagattt
     9961 ttccgccgct ctggcacata ttgaaactga aatgaccgca accgtggacg aggtatataa
    10021 cgagatcatc ctttgcgtac ccatgagcaa ccccgccgcc gtggattatg tcatcaggcg
    10081 cggataccac acgaatgctt tttttgaaca cttccttacc gacaagccat tgggtcatta
    10141 tgaaaactat atttttatag acccgatcct gaccacatga ttttttgaat ttcaagcgcg
    10201 aatctgcttt ggaaagataa ctttgtgcta taatccgcgc acgatggtca accacatcca
    10261 attttccaat aacaataata atgacaatga cgatccccga atcgtagggc gaagcgttgc
    10321 tggttgacaa ataacaaaag tcgaaaccaa atcgcccgac gcaaatgcga agggcgattt
    10381 attttatata caccattaag actcaggtct aatggaaata tttcggaggc gcactatgta
    10441 tagatctaat ttatgtggag aattacgagc cagtcatgcg ggtcagaccg tgactttgtc
    10501 cggctgggtg aatcgacgcc gtgaccatgg cggggtggca ttttttgacc tgcgtgaccg
    10561 tgcaggcatc gttcaggtca ccatcaaccc agaccttcca aaggaagtgc tcgatcaggt
    10621 tgccagcgtt cgcaacgagt gggtcttgca gatcgaaggc gtggtgca
//
LOCUS       DAOQJC010000133        10611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_3230_length_10611_cov_10.922793, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000133 DAOQJC010000000
VERSION     DAOQJC010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10611
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3230_length_10611_cov_10.922793"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PLE39_15980"
     CDS             <1..332
                     /locus_tag="PLE39_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNE69882.1"
                     /translation="WGEAADLIVKGMEGAIHAKTVTYDFARLMDKPTEVKCSEFGDAI
                     IHWMDKEIPAPKAEAAAAKAPAKKAVAKKAVAKKPVKKAAAKKPAKKAVAKKTTKKVV
                     KKTGKKK"
     gene            436..786
                     /locus_tag="PLE39_15985"
     CDS             436..786
                     /locus_tag="PLE39_15985"
                     /inference="COORDINATES: protein motif:HMM:NF018672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HNE69883.1"
                     /translation="MNIVVNIITGLVAVLHGYFLILEMFLWEKPAGRKSFRTTAEFAA
                     QSKTLAANQGLYNGFLSAGLIWGIFAGDAVKVFFLSCVIIAGLFGAFTVSKRIFYIQA
                     LPAIIGLVILLVAG"
     gene            complement(871..2259)
                     /locus_tag="PLE39_15990"
     CDS             complement(871..2259)
                     /locus_tag="PLE39_15990"
                     /inference="COORDINATES: protein motif:HMM:NF012262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNE69884.1"
                     /translation="MNEETKKKINARYEKELNKGERFWPDSIFKDLVVSLGIFILLVL
                     LASFVGVAPEPKADPSDTSYIPRPEWYFLFLFKFLALYGQIPLLGKIEWLATVLVPGI
                     AIGVLTLLPFIEKSPDRHYAKRALSISVMTIMVVGIILMTLMSEIPTVSADGSKLLGL
                     LQAMAGLAVPGLAMIALFVAALAFKTTNTRFFIWTTSLTVVSMIILSGTVMALHTPPA
                     VEETEVATTLVDQIFAGQDLYSVHCTECHGDDGSVAVIEGVEGLEGEKITPINSHDVL
                     YTITDSAMYEVIAYGRPNAGMTPFGKAYGGELSKSEIDYIVTYMRYMWDDRFEIPAEA
                     LKPLFPPLAEGEVPSYEVHIQPIVKRYCVSCHRAGKENNNYLMTSYEEMLTTGDNAAN
                     NIIAGDENSYLLQVIQEHAITDANGQELIGVMPPNRALKPNIVDVFIRWIMNGMPQTA
                     VDAAALFTAPAP"
     gene            complement(2289..2948)
                     /locus_tag="PLE39_15995"
     CDS             complement(2289..2948)
                     /locus_tag="PLE39_15995"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNE69885.1"
                     /translation="MWKTVYEWLDERLGLNDLYTNLLDRPEPKGNWWNTLGSASLFLF
                     VLQGLTGIFLTVYYTPSPDHAYDSVQYIMEGVAFGWLIRGIHHWGATLMVIVVFIHLL
                     RVFITASFKFPRELTWLIGIGLFLATLGMGFTGYLLPWNQKAYWATTVGTQIAGSVPF
                     IGEFILKALRGGPDLSALTLQRFFSAHIWIIPAVLAGLIGIHLYLIIKHGESHFPTKE
                     D"
     gene            complement(2952..3449)
                     /locus_tag="PLE39_16000"
     CDS             complement(2952..3449)
                     /locus_tag="PLE39_16000"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNE69886.1"
                     /translation="MSGSNELSRRDFIKVTTGIVGGLIGVALGLPSVSYLIDPALQEG
                     GKDAWISIGKFEDMQLEKPYPFSFTRVQVNGWERTSSSFGGYAIRKSDDPKDLLILNS
                     RCTHLSCNVNWHEESDAFLCPCHDAKFSRDGNVLDGPPPRPLDVYEEFRLSEDGNIEI
                     FFKAG"
     gene            3831..4205
                     /locus_tag="PLE39_16005"
     CDS             3831..4205
                     /locus_tag="PLE39_16005"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69887.1"
                     /translation="MGLKRNVSLSTGLRYKGQGPMLTFVLHRIGGLGMAVFITTHILA
                     SFLGGKGGAFVNSIYENWVFQIFIFFCVLFHAINGLRITILDLFPKLIDHHREAIWIE
                     WAVFIPVYAISVFVIVTTALGG"
     gene            4207..4641
                     /locus_tag="PLE39_16010"
     CDS             4207..4641
                     /locus_tag="PLE39_16010"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69888.1"
                     /translation="MTTKTRTLSIKQRGLTNFESIMWIFTRLSALAMYALIIFGMIGA
                     LVMGAREQMNFADVMRWAFMPNVTHVQSTDVPDLNPWSSPFWKLTASALLFVAVAHGV
                     HGLVVIADDYITTEGGRKIVRLLSIIMMGSMSLMGLYLIWTS"
     gene            4722..6515
                     /locus_tag="PLE39_16015"
     CDS             4722..6515
                     /locus_tag="PLE39_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE69889.1"
                     /translation="MANVHQFEVVVVGAGGAGLMAGLYASRGAKTAVISKLYPTRSHT
                     GAAQGGISAALGNYEEDKPEWHMYDTVKGSDYLGDQDAIEFMTNEAIDAVLELEHMGL
                     PFDRTPEGRISQRPFGGHTNNVTGKPVRRAAHAADRTGHMILQTLYQQCIKNDVTFFD
                     EYQVLDFIMVDGKAAGVVAIELATGELHTFHAKAVIFATGGHGRIFEVTSNAYAYTGD
                     GAAILLRHGIPLEDMEFFQFHPTGIYKLGILITEGVRGEGGILINGNGERFMPKYAPT
                     VKDLASRDVVSRAIYTEIKEGRGINGSNYVYLDIRPESVNKFAALDGRTNPDGTPYTI
                     TGETVMQKIPDIVDFCRVYLGVDPVTQVMPIQPTAHYTMGGIPTNKFGEVVTDDKGTA
                     LPGLFAAGECACVSVHGANRLGTNSLLDLVVFGKHAGLRAAEYAKSAEWVKMPEDAEA
                     GTRSALEALRKGSGKENAFDIANEMKKVMFADVGIYRNEKDMQSALEKVRELQGRFKN
                     IGVTDTGKIFNTELLNAWELGNMLEIAEVVAASALNRKESRGGHSREDFPDRDDANWL
                     KHTLIAKKNGKLEINYKPVVITKHQPKARVY"
     gene            6524..7231
                     /locus_tag="PLE39_16020"
     CDS             6524..7231
                     /locus_tag="PLE39_16020"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HNE69890.1"
                     /translation="MEVTIKIFRYNPEKDKRGNYVTYKVEADENDRVLDVLEFIKGKT
                     DGTLSFRRSCAHGVCGSDAMRINGRNMLACKTLIRDVGANITVEPVLGLKVKKDLIVD
                     MEPFFDNYRKMLPYFINDSPLPENGRERLQSPEQRARFDETTKCILCAACTTSCPSFW
                     ANEEYYGPAAIVTAHRFIFDSRDEAADKRLQVLAETDGIARCHTAYNCTEACPREIKI
                     TKAIGEVKMAIITGKLD"
     gene            complement(7387..7983)
                     /locus_tag="PLE39_16025"
     CDS             complement(7387..7983)
                     /locus_tag="PLE39_16025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE69891.1"
                     /translation="METIRGRLSHFLDSTELQARLNPYNRLTLDLGTGDGKFAFCRAD
                     AFPSHFVIGVDSCRENLHEYSRAKLPNLLFIIASAQSLPHELHGLASHITINFPWGSL
                     LESLLNGDDRLLHGLQSVARSPAALDIRLNGGALAEQGWALEEGTERIFENLTSTGWK
                     IRPPEMLDSHALRAFPSTWAKRLAFGRDPRAMQLSGCL"
     gene            8298..9326
                     /locus_tag="PLE39_16030"
     CDS             8298..9326
                     /locus_tag="PLE39_16030"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE69892.1"
                     /translation="MRYQLLGRSGLRVSEASLGTMTFGETWGWGASKAESKKMFDLFT
                     KAGGNFIDTSFNYTDGTAEEFLGDFLKTDRDYFVLASKYTLTKPDNTDPNGGGNSRKN
                     MMRSVEQSLKRLKTDFLDIYYLHVWDYMTPVDEVVRGLDDLVRQGKVNYVAFSDSPDY
                     IVAEANTRAELMGWSRFIGMQIPYSLLDRAVERSVIPMAAHWEMAVLPWGLLEAGILT
                     GKFLKSIKNSTRLNPKKLRLSEKQLSVVQEVQRISEETGKPMSQVAINWVRQSPKAQM
                     IPILGARTASQLKDNLAAFDWKLDDDQYQRLEKVSAIEMGFPYDFIPGNPYVYGATFD
                     KIDRPIRR"
     gene            9630..>10611
                     /locus_tag="PLE39_16035"
     CDS             9630..>10611
                     /locus_tag="PLE39_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69893.1"
                     /translation="MKRISHLLILLVVLSTACNLPGSLTAQAETLPLETATQPGAESE
                     SGEAILLFTIGMHIEPMGETAQGYKSGKGDHHNPIFFDKQVQDIRTVTQIVEAHGGRM
                     TIQAQSPFTTVAIESGNSILADLAASGHEIGLHFHEDAHLGKNSETLTVQTWCDVMSE
                     EIELIEQAASVEKIRFWSGGNLNPNLYEAAECAGLSVNSDWKNRMTQETPLDLVGVNP
                     WRPAGGTDGTDFNLFTRHDPHGAIIFLPEGQYDKTNFASMRRSEDTGGDEAYFEFLKQ
                     SLYASLDAAEAGEVNVFHFTIHPGEFRGDPQHPFEVIEKFLSEVVDPLVAS"
BASE COUNT         2632 a         2606 c         2953 g         2420 t
ORIGIN      
        1 gttggggcga agctgccgac ttgatcgtga agggcatgga aggcgccatc cacgccaaga
       61 ccgtcaccta cgattttgcc cgcctgatgg acaaacccac cgaagtgaag tgctctgaat
      121 tcggcgatgc catcattcac tggatggaca aggaaattcc cgccccgaaa gcggaagctg
      181 ccgctgcgaa ggcacccgcc aaaaaagccg ttgccaaaaa ggctgttgcg aaaaaaccag
      241 tgaagaaggc tgccgcaaag aaacctgcaa agaaggcagt tgcaaaaaag acaaccaaga
      301 aggttgtaaa gaagactggc aagaagaagt aaatcaaaag ttggaatcaa aggacggagt
      361 cggctgcgac tccgtccttt ttctttttat ggggtatcat ccattcaaat tcagacacaa
      421 cttaaaggag aaacaatgaa tatcgtcgta aacatcatta cgggtttggt ggctgttttg
      481 catggatatt tcctgatcct cgaaatgttt ctgtgggaaa aacccgcagg ccgtaaatcc
      541 tttcgcacta ctgcagagtt tgcggcacaa tccaaaacgc tggcggcaaa tcaggggctg
      601 tacaacggtt ttctctcggc aggtttaatt tgggggattt tcgccgggga tgccgtgaag
      661 gttttcttcc tgagttgtgt catcatcgcc ggtctttttg gcgcattcac ggtcagcaag
      721 cggatatttt atatccaggc tttaccagcg atcattggtc tggtgatcct gctggtggca
      781 ggctgaaacc actcccaatg tctttgcttt aaatcaaaaa agccgtgacc ttacatcacg
      841 gcttttttga ttcttgttag caaacaccat ctatggcgct ggtgcagtga acaacgcggc
      901 agcatcaacg gcggtctgcg gcataccgtt catgatccag cggatgaaga catcaacgat
      961 attgggtttg agcgcgcggt tgggcggcat gacaccgatc aattcctgtc cattggcatc
     1021 ggtgatggca tgttcctgaa tgacttgcag gaggtagctg ttttcatcac ctgcgataat
     1081 gttgttggca gcgttatcac ctgtagtgag catctcttca taagaggtca tcaggtagtt
     1141 gttgttttcc ttgccggcac gatgacagga gacacaatat cgcttcacaa tgggctgtat
     1201 gtggacttca tacgaaggca cttcgccctc tgccaatgga gggaacaacg gtttaagcgc
     1261 ttcagcaggg atctcgaaac ggtcatccca catgtagcgc atataggtga cgatgtaatc
     1321 aatttcgctc ttggagagtt caccgccata cgccttgccg aatggggtca tacctgcatt
     1381 ggggcgccca taggcaatga cctcgtacat ggcagagtcg gtgatggtgt agagtacgtc
     1441 gtggctgttg atgggggtga tcttttcgcc ttcgagacct tccacacctt caatgaccgc
     1501 aaccgaacca tcgtcaccgt ggcattcggt gcaatggacg gaatataggt cctgtccggc
     1561 gaagatctgg tctacgaggg tggtggcgac ttcggtctct tcaacggcgg gcggagtgtg
     1621 cagagccatc actgtccctg agagaatgat cattgaaaca acggtcaggc tcgtcgtcca
     1681 gatgaagaag cgggtgttcg tcgttttgaa ggcgagagcg gcgacaaaca aggcgatcat
     1741 ggccaggccg ggaacggcga gtcctgccat ggcttgcagc aggccaagca atttcgatcc
     1801 atcggcagag acggtgggaa tttcagacat cagggtcata aggataatgc ccacgaccat
     1861 gatggtcatg accgagatgg agagggcacg ttttgcgtag tgacggtcag ggcttttttc
     1921 gatgaagggg agcagggtga gtacgccaat ggcaatgccg gggacaagta cagtggcaag
     1981 ccattcaatt tttccaagca gggggatttg cccgtacagg gcaaggaact taaacagaaa
     2041 gaggaagtac cactcagggc gcggaatgta ggaggtgtcg ctggggtcgg ccttgggttc
     2101 cggagccacc cctacaaagc ttgccagtaa caccaacagg atgaagatac cgagcgagac
     2161 aacgaggtct ttgaaaatgc tgtctggcca gaagcgttcg cctttgttca gttccttctc
     2221 gtatcgtgcg ttgattttct ttttggtttc ttcgttcatg gttgtatgcc ttgtatgggg
     2281 aagaatcgct aatcttcctt tgtggggaag tgcgattcac catgtttgat gatcaaatac
     2341 aagtgaatgc cgatcaggcc agccagtacg gcagggatga tccagatatg tgctgagaag
     2401 aagcgttgca aggtgagcgc gctcaaatca ggtccgccgc gcagggcttt gaggatgaat
     2461 tcgccgatga agggaacact gcctgcgatc tgtgtgccga ccgtggtggc ccaataggct
     2521 ttttggttcc aggggagcag gtagcctgtg aagcccatgc caagtgtggc aaggaacagg
     2581 ccaatgccga tgagccaggt caactcacgc ggaaatttga aagaagcggt gatgaacacc
     2641 cgcagaaggt gaatgaaaac cacgatcacc atcagggtgg caccccaatg atgaatgccg
     2701 cgaatcaacc agccaaaagc gacgccttcc atgatgtatt gcacggaatc ataggcatgg
     2761 tcgggggagg gggtgtaata gacggtgagg aagataccgg tcaggccctg taggacaaag
     2821 aggaaaaggc tggctgagcc aagggtgttc caccaattgc cttttggttc agggcgatcc
     2881 agcaggttgg tgtaaaggtc gttcaagccg agacgttcat caagccattc gtaaactgtt
     2941 ttccacatga tttagcctgc cttgaaaaag atttcgatgt tgccgtcttc tgagaggcgg
     3001 aattcttcat atacatccag cgggcgcggc ggggggccat cgagcacgtt tccatccctg
     3061 ctgaatttgg catcatgaca ggggcagaga aatgcatctg attcttcgtg ccagtttaca
     3121 ttgcaagaca ggtgtgtgca gcggctattg aggatgagca ggtctttggg gtcatctgat
     3181 ttgcggatgg cgtacccgcc aaacgaactg gaggtccgct cccagccatt gacctgtacc
     3241 cgcgtaaacg agaacggata aggcttttca agctgcatat cttcgaactt accgatggaa
     3301 atccatgcgt ctttcccacc ttcctgcagg gctggatcta ttaaatacga aaccgaaggc
     3361 aacccgagcg caaccccgat taaacctccg acgatcccgg ttgtgacctt gatgaaatcg
     3421 cgccgggaaa gttcattcga acctgacatg tacgcctcct tggacttttg gaaataactt
     3481 ctgtcgcttg ccttatagat cgccagattt ttgcatggat ttacgggtaa taatcacagc
     3541 tttggtctgg gaaatcgtgc gatactctgt agtcagggaa tattaaagat gatgtggcgt
     3601 taagtgaatt ggattataag ggtctttctc cgctttatta taggatagct gaaatgaaat
     3661 atggatgaca attgtcacca tattttgcgg tttcaaagtt aaaagagtaa aataggccct
     3721 tgtatcataa gagaccataa acgcatgaac atgggatttg tctgatcatg ggggctttgt
     3781 gccattttca agcaggcctc ttatgaaaaa attcttttga ggaggcatag atgggcctga
     3841 agagaaatgt cagcctatcc acgggactga ggtacaaggg gcagggtccg atgttgacgt
     3901 tcgtcctgca ccgcattggc gggctgggaa tggcggtctt catcaccaca catattctgg
     3961 cttcgttttt gggtggaaaa ggcggagcgt ttgtgaacag catttacgaa aactgggttt
     4021 ttcagatttt cattttcttt tgcgttttgt tccatgccat caacggcttg cggatcacga
     4081 tcctcgacct gttccccaaa ctgatcgacc atcatcgtga ggccatctgg atcgagtggg
     4141 cggtattcat tcctgtgtat gccatctccg tttttgtcat tgtgaccacg gcactgggag
     4201 gctagaatga ccacgaaaac aagaacactc tccattaaac aacgcggatt aacgaacttc
     4261 gaatccatca tgtggatttt tacgcgtctt tccgccctgg ccatgtatgc cctgatcatc
     4321 ttcggcatga tcggcgccct tgtaatgggc gcacgcgaac agatgaactt tgcagatgtg
     4381 atgcgctggg ctttcatgcc caatgtcacc cacgtgcaaa gcaccgatgt gcccgatctc
     4441 aacccatggt cttccccatt ctggaagtta accgccagtg cgctgctgtt cgttgcagtt
     4501 gcccacggcg tgcatgggct ggtggtcatt gcagatgact acatcaccac cgagggcggc
     4561 cgcaagatcg tccgcctgct cagcatcatc atgatgggtt ccatgagtct catgggcttg
     4621 tatctcatct ggacttcgta aaaagattca tcacggataa atgcaggtga aaaatctttt
     4681 atctgtggtt aaaacctaaa aggttttctc aggagtaatt catggcaaat gttcatcaat
     4741 ttgaagtggt tgtggttggc gcaggcggtg caggcctcat ggcaggtctg tatgcttcgc
     4801 gcggtgccaa gaccgctgtt atcagcaagc tgtaccccac ccgctcgcac accggtgcgg
     4861 cacagggcgg catttctgcg gcgctgggca attacgaaga agacaagccc gaatggcaca
     4921 tgtacgacac tgtcaaaggc tccgattacc tgggcgacca ggacgcgatc gagttcatga
     4981 ccaacgaagc gatcgacgct gtgctcgaac tggaacacat gggcctgccc tttgaccgca
     5041 ctcctgaagg ccgcatctca cagcgacctt tcggcgggca caccaacaat gtcaccggta
     5101 aacccgtgag aagggccgcg catgccgccg accgcacagg ccacatgatc ctgcaaacac
     5161 tgtaccagca gtgcatcaag aacgatgtga ccttcttcga tgaatatcag gtgctcgatt
     5221 tcatcatggt ggatggaaaa gctgcgggcg ttgttgccat tgagcttgcc accggtgaat
     5281 tgcacacctt ccacgcgaag gctgtcatct tcgccaccgg cggccatggc cgcatcttcg
     5341 aagtcacctc caatgcctat gcctacactg gcgatggcgc ggccatcctg ctgcgtcatg
     5401 gcattccgct cgaagacatg gaatttttcc aattccaccc gacagggatt tacaaactcg
     5461 gtattctcat cacagaaggt gtccgcggtg aaggcgggat tctgatcaac ggcaacggcg
     5521 aacgttttat gccgaaatac gcccccaccg tgaaagacct tgcttcacgc gatgttgttt
     5581 cgcgtgcgat ctacactgag attaaagaag gccgcggcat caacggctca aactatgtat
     5641 atctggatat tcgccctgaa agcgtgaata aattcgccgc attggacgga cgcaccaacc
     5701 cagacggtac accgtacacc attaccggcg aaacggtcat gcagaaaatt ccagatattg
     5761 tggatttctg ccgcgtatat ctcggtgtgg accctgtcac ccaggtgatg ccgatccaac
     5821 cgacggcgca ttacaccatg ggcggcattc cgaccaacaa gttcggtgaa gtggtgacgg
     5881 acgacaaagg tacagcgctc cccggtttgt ttgcggcagg tgaatgtgct tgtgtctctg
     5941 tgcatggcgc aaatcgtttg gggacgaact cgttgctcga cctggtggtg ttcggcaaac
     6001 acgcaggtct gcgtgcagcg gaatacgcca agtctgctga atgggtgaaa atgcccgagg
     6061 acgccgaagc aggtacaagg tccgctttgg aggcgctgcg caaggggtct ggcaaggaaa
     6121 atgcgttcga cattgccaac gagatgaaga aggtcatgtt cgcagatgtc ggcatttatc
     6181 gaaacgaaaa agatatgcaa tctgcattgg aaaaagtgcg cgaattgcag gggcgcttca
     6241 agaatatcgg cgtgacagac acgggcaaga tcttcaacac cgaattgctg aatgcatggg
     6301 aactcggcaa catgctcgag atcgcagaag tggtggcggc cagcgcattg aaccgcaaag
     6361 aatcccgcgg cggacattca cgcgaggatt tccccgaccg cgatgacgcc aattggttga
     6421 agcatacgtt gatcgcaaag aagaacggaa aacttgagat caattacaag cctgttgtca
     6481 tcaccaaaca ccagccgaag gcaagagtgt attagaggta aacatggaag ttacaatcaa
     6541 aattttccgt tacaaccctg agaaggacaa gcgcggcaat tacgtaactt acaaggtgga
     6601 agccgatgaa aacgaccgcg tgctggatgt gctcgaattc atcaaaggca agaccgatgg
     6661 cacgttgtcc ttccgccgtt cgtgcgcgca tggcgtgtgc ggttcggatg cgatgcgcat
     6721 caatggccgc aacatgctgg cctgcaaaac cctcatccgt gatgtgggcg caaacatcac
     6781 tgtggagccg gtgctcggct tgaaggtaaa gaaagacctg atcgtggata tggaaccgtt
     6841 tttcgataat tatcgaaaaa tgctgccgta tttcatcaac gacagcccgc tccccgaaaa
     6901 tggacgcgaa cgactgcaaa gcccggaaca gcgcgcgcgt ttcgacgaga ccaccaagtg
     6961 cattctttgt gcggcctgca ccacctcctg cccgtcgttc tgggcgaacg aggaatatta
     7021 cggcccggcg gccattgtca ccgcgcatcg cttcattttc gacagccgtg atgaagccgc
     7081 agacaaacgt ttgcaggtcc ttgccgaaac agacggcatt gcccgctgtc acacggcgta
     7141 caactgcacc gaagcctgcc cgcgtgagat caagatcacc aaagccatcg gcgaagtgaa
     7201 gatggccatc atcaccggca agctggatta aagtacactg cgcttcaaaa cgcaccctga
     7261 aactgattct gttcaaataa agagaagacc tcctgtgttt ttcacaggag gtcttttgcg
     7321 tcagatgggg aaataggatc aagaatcgga ggaagacaaa tcatacttgc ggtatgactt
     7381 gttttcctaa agacacccgc tcaattgcat tgcccgtgga tcacgcccga aggcaaggcg
     7441 tttggcccag gtactgggga acgcccgcaa agcgtgcgaa tcgagcattt caggcgggcg
     7501 gatcttccaa cctgtggagg tgagattctc aaaaatgcgt tctgtgccct cttcaagagc
     7561 ccagccctgc tcagcaagtg cgccgccatt caagcggata tccaatgcgg ctggcgaacg
     7621 tgccacagac tgcaaaccat gcaagagtct gtcatcgcca ttcaacagac tttcaagcag
     7681 gctgccccac ggaaagttga tggtgatgtg tgaggcgagt ccgtgcaatt catgcggcag
     7741 ggactgtgcg ctggctatga tgaagagcag gtttggcagt ttggcgcgtg aatactcgtg
     7801 caggttttcg cggcacgagt ccacgccgat gacgaagtgg ctggggaatg catcggcgcg
     7861 gcaaaatgca aatttgccgt caccggttcc aaggtccagt gtgaggcggt tgtaagggtt
     7921 gagtcgcgcc tgtagttcgg tcgaatctaa aaaatgggac agtctgcccc gaattgtttc
     7981 catgtgaaag ctccaatctg tctaatgggt tgaatagaga caggttggag ttgggggatc
     8041 acgggcgcgt gaagtgaaac ggctgcctgt acgtcaggca ccgtcgagat catcgggagt
     8101 gggtttatgt ttggttacgg ggaatgtaaa acgggcccgc tgaaagtggg ccttgtgaac
     8161 cgttgattca gttttaattt agcagggtgg tcgggttacg ttcacaggcc gattgtagca
     8221 tgatgtctgc ggcgggcatg ttggaatttt gtatgcaaat cggtatgcta tactgaaaaa
     8281 aattatagga ggacgcgatg cgttatcaat tacttggccg cagcgggctg cgcgtttcag
     8341 aagccagcct tggcacgatg acttttggtg aaacctgggg gtggggcgct tcaaaggctg
     8401 aaagcaaaaa gatgttcgac ctgttcacaa aggcgggtgg aaatttcata gatacctcgt
     8461 tcaattacac agacgggact gccgaggaat ttctcggcga ttttctgaaa acggatcggg
     8521 attactttgt gttggccagc aaatacacgc tgaccaaacc cgacaacacc gacccgaacg
     8581 gcggcgggaa cagccgcaag aacatgatga ggtcggtgga acaaagcctg aagcggttga
     8641 agaccgattt cctcgatatt tattatttgc atgtgtggga ttacatgacg ccagtggatg
     8701 aagtggtgcg cggactcgat gacctggtgc ggcagggcaa ggtgaattat gtggcttttt
     8761 ctgattcgcc cgattacatt gtggcggagg ccaacacccg cgccgagttg atggggtggt
     8821 cgcgttttat tggaatgcag attccctatt cactgctgga ccgtgccgtg gaaaggtcgg
     8881 tgattccgat ggcggctcat tgggagatgg cggtgctgcc gtggggcctg ctcgaagcgg
     8941 ggattctgac gggaaaattt ttgaagagca tcaaaaactc cacgcggttg aatccgaaaa
     9001 aacttcgctt gagcgaaaag caattaagcg tggtgcagga agtgcagcgg atttcggaag
     9061 agactggcaa gcccatgtcg caagtggcga tcaattgggt gaggcaatct ccgaaggcgc
     9121 agatgattcc cattcttggg gcgcgtacgg ccagtcagtt gaaggataac cttgccgctt
     9181 tcgattggaa attggacgat gaccaatacc agcggcttga aaaagtcagc gccatcgaaa
     9241 tggggttccc gtatgatttt attccgggca atccctacgt gtacggcgca acgttcgata
     9301 aaatagaccg ccccatccgc agatgaccac ggaagtttga gcgggggcat cacaaagccg
     9361 cgacacaggg ttgcaaggga aatttccttt gtgaccctgt gttgtttttt ctaaaaagaa
     9421 ttggtgatgg gtcgaaataa atgacaatgt ccgtagcgcg agatagtatc tcgcgctaca
     9481 tcatttacac aaacccatta ccaaaccttc ttttagccgc tcccaaaaac aaactgtcag
     9541 gtgactggct ttttttacaa tcattttacc gggggaaaac tgtgcggtca tgctgaaact
     9601 gtatagtaca agcgaaagga gcgcaaacca tgaaacgcat ttcccacctt ttgatcctat
     9661 tggtcgttct ttccacggcc tgcaacctgc cgggcagcct cactgcccag gctgagactc
     9721 tgccgcttga aaccgccacc cagcccgggg ctgagtcaga atcgggcgaa gccatcctgc
     9781 tgttcaccat cggaatgcac atcgaaccga tgggtgaaac cgcgcaggga tacaaatcgg
     9841 gcaagggcga tcaccacaat cccatttttt tcgataagca ggtgcaggat attcgcactg
     9901 tgacgcagat cgtcgaagcg catggcgggc gtatgaccat tcaggctcaa tcgccgttca
     9961 ccacggtggc gatcgaatcg ggtaattcca tcctcgccga cctcgcagcc agcggacatg
    10021 agatcgggct gcacttccat gaagatgcgc acctcggcaa aaacagcgag accctcaccg
    10081 tgcaaacatg gtgcgatgtg atgagcgagg aaattgaact cattgaacaa gccgccagcg
    10141 tggagaaaat ccgcttttgg agcggaggga atctcaaccc gaatttgtac gaagccgccg
    10201 aatgtgccgg gctgagcgtg aacagtgact ggaagaatcg catgacacag gaaacgccgc
    10261 tcgaccttgt aggtgtgaac ccgtggcgtc ccgcaggcgg caccgacggc acagacttca
    10321 acctcttcac ccggcacgac ccccatggcg cgatcatctt tctgcctgaa ggccagtatg
    10381 acaagaccaa ttttgcctcg atgcgccgca gcgaagatac aggcggcgac gaagcctact
    10441 ttgaatttct caagcaaagt ttgtatgcct cgctcgatgc cgccgaagcg ggcgaagtga
    10501 acgttttcca tttcaccatt caccccggcg aatttcgcgg cgacccgcag catcccttcg
    10561 aagtgatcga aaaattcctg agcgaagtag tggacccgct cgtcgccagc g
//
LOCUS       DAOQJC010000134        10517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_3277_length_10517_cov_8.389983, whole genome shotgun sequence.
ACCESSION   DAOQJC010000134 DAOQJC010000000
VERSION     DAOQJC010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3277_length_10517_cov_8.389983"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="PLE39_16040"
     CDS             <1..1017
                     /locus_tag="PLE39_16040"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNE69894.1"
                     /translation="IGGLIMGVGFIIGGFCPTTSLASASTGKIDGMMFMLGGFVGAAL
                     FAESEPLFTNWYNNAGYFGRVTLDEVFGISKGAVVVLVILMALFMFWGSEQLERIFGK
                     KDLSKEPKIRVAGAAVLAVMAFAVFFIGSPSLEDKYNKLTFTRTETIEQLNADPIVET
                     KIYTADEMLSNRLVFVSPAEAFKTKYAQTVNPVYLDVRPEADFNLYHLEDAVNVPLDR
                     LTEVIPVLLTEPPANTVYILMSNDEAAAVQAWKTLVASSVPNVYILEGGINNWIAFFG
                     KDDKALQPNPNAGDDQLGYTFPAALGSRYDSCSPNPQEYEELDFEAKIKLQLKRDKSG
                     GGCG"
     gene            complement(1102..2604)
                     /locus_tag="PLE39_16045"
     CDS             complement(1102..2604)
                     /locus_tag="PLE39_16045"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE69895.1"
                     /translation="MSWMDTLMKQAGKLNPRVLAFLEKRMKNIPSVAKKIEGEYAEIM
                     TELETSVKPYKNKFTTFSKIPAKGRPHTDILNEMEAMRALEESHWKDGFISGAVYHGD
                     QAHIDFLNQVYALNSQSNPLHADVWPSATKFEAEIVSMTAAMLGGAGVDACGTLSSGG
                     TESIMLAMKTYRDWAREVKGITRPEMIVPVTAHAAFDKASQYFNIKTIHVPVDENFRA
                     DLKAVKKAISKNTIVIVGSAPSFPHGAIDPIAEMSELARQHNIGFHTDACLGGFVLPW
                     AEKLGYDVPAFDFRLAGVTSISVDTHKYGYAAKGTSVILYRNQELRRFQYYTTTEWPG
                     GLYFSPTFAGSRPGALSAACWAALTSIGEEGYLAATKRILETAEKIKQGIREIPELYV
                     LGDPFFVVAFASESLNVYKVLDHMAHKKWSLNGLHKPTCVHLCVTLRHTQPGVAERFL
                     TDLREAVEHVKGHPEEKGSMAPVYGMAATIPMRGMVSDLLKKYLDLIFKV"
     gene            complement(2660..4243)
                     /locus_tag="PLE39_16050"
     CDS             complement(2660..4243)
                     /locus_tag="PLE39_16050"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNE69896.1"
                     /translation="MSSTSKFILAIDLGTSGPKVALFSTQGELIGSEFQETPVILLPN
                     GGAEQSPAGWWSAIDTACKRLLAKKLVSSDDIVAIANTTQWSGTVAVDADGNAIENAV
                     IWMDSRGAPYIKKIADGFPKIEGYGLSKVLNWIRLTGGAPGNSGKDPLAHILYLKDVR
                     PDIYQRTYKFLEPMDYIGLLLTGEFAASFNSITLHWMTDNRDIQNVKYHDGLIQLAGI
                     DRAKLPDLKPANAQLGTLRREIASAWGLRDDVKVFMGSPDLHSAAVGSGAVRDFEPHL
                     YVGTSGWLVCHVPFKKTDIFHNLAALPSGIPGRYLLTNEQESAGGCLQYLRNNILFPQ
                     DELSVGEKPANAYQLLDQMAERTPAGSGKLIFTPWLYGERTPVEDHLVRGGFHNQSLS
                     TTRADMVRAVFEGVAYNARWLLQYVEQFNKRPVESINMVGGGAKSNIWCQIHADVLNR
                     PIRQVKDPIEANVRGAALLASAGMGYLKYDEIGTHVKIAATYVPNPDNRKIYDELFRE
                     FLAIYESNRKIHARLNRMH"
     gene            complement(4324..4566)
                     /locus_tag="PLE39_16055"
     CDS             complement(4324..4566)
                     /locus_tag="PLE39_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69897.1"
                     /translation="MTETFTKGESHGCIMCGKLYQLYVVTDSKGKFVDAKVMSAGGRL
                     VKHPQRALVACETHSNAEIEAAVARAYGVQKEDDDD"
     gene            complement(4674..5366)
                     /locus_tag="PLE39_16060"
     CDS             complement(4674..5366)
                     /locus_tag="PLE39_16060"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE69898.1"
                     /translation="MAKKKNVKNAREAKSDRTIQYVVVALALIVLGFIGWPYLPKPKS
                     NVAVGTTPACEVFDSIAAAEQYTSAPPLSIDESKPYFATFKMEKGGEFVVQLYSDKAP
                     VTVNNFVFLACKGFYNGVTFHRVMDGFMAQGGDPTGSGMGGPGYSFEDEISDLTFDRE
                     GLLAMANSGPNTNGSQFFITYAPTDWLNGLHTIFGEVVSGMDVVNAITRRDPQQNPGV
                     EGDVIESIIITQ"
     gene            5412..6281
                     /locus_tag="PLE39_16065"
     CDS             5412..6281
                     /locus_tag="PLE39_16065"
                     /inference="COORDINATES: protein motif:HMM:NF025615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HNE69899.1"
                     /translation="MVQQPCAQANSLVCLPNMQQYFKKQIALPQDHGSWVFIFSPLLI
                     GIFAGKHFTYATFNLIIAAMSAFMIRQPMTVIVKTISGRRPKTDLPAARFWLLVYGGI
                     ATLALLGLILEGFAYILYLAIPGAPVFAWHLWLVSKRSERRQSGVEIIATGVLALVAP
                     AALWVGLGHYDPAGWWLWVWAWLQSAASIVYAYLRLEQREQVEGQERSALWKAGRRAF
                     LYTSFNLILSLLLGWAGLIPQLIFAAFLVQWLETLWGITHPATGWKPVKIGMRQLIVS
                     ILWTVLFIVFNHA"
     gene            6305..>7131
                     /locus_tag="PLE39_16070"
     CDS             6305..>7131
                     /locus_tag="PLE39_16070"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE69900.1"
                     /translation="MDFDLNEEQKAWKKAVHDFVGKVVKPKSREVDETESFNWEAVRK
                     GGPLGLMGLSIPEAYGGSGVDMVSSSIAMEELGWGCGSTALAFTAHNGLGTAPIALYG
                     SEELKKKWLPLVAEGKNKLGALALTEPGAGSDIQGGVITRAEKQGDEWVINGAKMWCT
                     NASIAEYIITLVRTDPKGGSKSLSMILVPTDSKGLTIGPPEKKMGLRGSPACGISYDN
                     VRVPLGNLVGAEGRGLQQTLATLDAGRISIGAISVGLAQAALEAAVDYARERKAFGQ"
     assembly_gap    7132..7231
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7365..7913)
                     /locus_tag="PLE39_16075"
     CDS             complement(7365..7913)
                     /locus_tag="PLE39_16075"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE69901.1"
                     /translation="MPFDHFAAIAPLYARVTYSKREIMREVAALPVKGRLLDVGGGTG
                     RVSSAIRDLVDEVVIADVSMGMLKETLPTPLRPVCGGSESLPFADESFERVIMVDALH
                     HVINHAESAREMFRVLKTGGVLVIEEPDIRTFGVKLIALAEKLLLMRSHFLAPDKIEK
                     LFEIGEKKIRAEDGTAWVIVRK"
     gene            complement(7913..8482)
                     /locus_tag="PLE39_16080"
     CDS             complement(7913..8482)
                     /locus_tag="PLE39_16080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HNE69902.1"
                     /translation="MKNVLYILLGIVAGFLLAGALIFVSRAPAGEPIVLRAAPTAAPI
                     KVHVIGAVPRPDVYDLPEGARVQDAINAAGGLLADANVDGLNLAALVVDGQQLSIPYK
                     DGAEPVETVELPGSETATPDPNAGEKININTATLEELDTLPGIGLTTAQKIIDYRLAN
                     GPFAKIEDIMNVSGIGPTTFEGLKDLITV"
     gene            complement(8492..8935)
                     /locus_tag="PLE39_16085"
     CDS             complement(8492..8935)
                     /locus_tag="PLE39_16085"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69903.1"
                     /translation="MSKESIRPALESESAQIKDLINLVGINPTGLDWKRFVVTVNDSG
                     QVIACGQIKPHGTDIRELASIAVHPDHRGKGLARAVIEVLLEQTERPVYLMCIAHNGP
                     MYEKFGFHTVPDDQMPKYFKRIKNLFDVAVLFRKTEENLLVMKLE"
     gene            complement(8937..9551)
                     /locus_tag="PLE39_16090"
     CDS             complement(8937..9551)
                     /locus_tag="PLE39_16090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNE69904.1"
                     /translation="MKYLAGERLKFDWRVVSVTIISTLLLMVDSYHKLTPYKYWDRII
                     LYLAIPMLVISIFFFEDPREYGFSFGDWKLGLAYTILGILLMAPIIYYLGHGNPVMRA
                     YYADYLPGLPWTTFLDLLGWEFFFRGWILFAYARRFGHEALWLQAVPFALAHISKPEV
                     ETLSTIFGGFAFGWVAWRTRSFVWPFLIHWFIATFVILVAAGAL"
     gene            complement(9548..10048)
                     /locus_tag="PLE39_16095"
     CDS             complement(9548..10048)
                     /locus_tag="PLE39_16095"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HNE69905.1"
                     /translation="MTFSFLNRKCKAREHPEGGCGVFATDFISKGELISLWGGDIVHR
                     SKLDPDMPRFTQRVLQIEEDLFILTSAAPEPNDCFNHSCDPNLGFTGQIGLVAMRDIS
                     AGEELCFDYAMSDGGPYDEFDCYCGSPLCRKKVTGNDWKLPELWVKYDGYFSSYLAKR
                     IEKIRK"
     gene            complement(10045..10464)
                     /locus_tag="PLE39_16100"
     CDS             complement(10045..10464)
                     /locus_tag="PLE39_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69906.1"
                     /translation="MLHPIWKFESDLTSRLLFWSILSIFSGLYIWFAADGFGRGFGIQ
                     AVVWGLIDAAIAIVGARTAARRKSTADPKREAIFIRKVLWINFGLDVLYILGGVWLIQ
                     NSVDGFWRGSGWGIVLQGAFLFFFDLFHAIKIPVELK"
BASE COUNT         2638 a         2886 c         2486 g         2407 t
ORIGIN      
        1 atcggcgggc tcatcatggg cgtaggtttt atcatcggcg gtttttgtcc cacgacttcg
       61 ctggcatctg cctccacagg caaaattgac ggcatgatgt tcatgctcgg tggttttgtc
      121 ggcgcggctc ttttcgctga aagcgaaccg ctcttcacca actggtacaa caacgcaggc
      181 tactttgggc gcgtcaccct cgacgaggtc tttggcatct ccaaaggtgc ggttgtcgtc
      241 ctcgtcatcc tcatggcatt gttcatgttc tggggttccg aacagctgga acgcatcttc
      301 ggcaaaaaag acctgagcaa ggaacctaaa atccgcgttg cgggtgctgc agtgcttgcc
      361 gtcatggcct tcgcagtctt cttcatcggc agcccatcgc tcgaagataa atacaacaag
      421 ctcaccttca cacgcacaga aaccatcgag caattgaacg cagatcccat cgtcgaaaca
      481 aaaatctaca ccgccgatga aatgctttcc aatcggctcg tatttgtcag cccagcagag
      541 gccttcaaga ccaagtacgc ccaaaccgtc aaccccgtct atcttgatgt acgccccgag
      601 gccgacttca atctctatca tcttgaagac gccgtcaacg taccgctgga ccgtctcacc
      661 gaagtcattc ccgttttgct caccgagcca cccgcaaaca ccgtctacat tttgatgagc
      721 aacgacgaag ccgccgccgt gcaggcatgg aagaccctcg tcgcctccag tgtgccgaat
      781 gtgtacatcc tcgaaggcgg aatcaacaac tggatcgcat tcttcggcaa ggatgacaaa
      841 gcgcttcaac ccaacccgaa tgctggtgac gaccaattgg gctacacctt ccccgcagct
      901 ttgggcagcc gctacgattc atgcagcccc aacccgcagg aatatgaaga gttggatttc
      961 gaagccaaga tcaagttaca gttgaaacgt gataaatctg gcggtgggtg cggataagaa
     1021 cggttttcag caatcaaata aagaaatcgc gcgagatcat tcgcgcgatt tctttattct
     1081 ttcctctttc ttctttcctg cctacacttt gaaaatcaaa tccaaatact tcttcaacaa
     1141 gtcactgacc atccctcgca tgggaatggt cgccgccatg ccgtagacag gagccatgga
     1201 acccttttcc tccgggtgcc ccttgacatg ctcaaccgcc tcgcgcaggt ccgtaaggaa
     1261 tctctctgcc acaccgggtt gggtatgccg caaagtgaca cacaaatgca cacaggtggg
     1321 tttgtgcaag ccattcaaac tccacttttt gtgtgccata tggtccagca ctttatacac
     1381 attgagcgat tctgaggcaa aagcaaccac aaagaacgga tcgcccaaaa cgtacaattc
     1441 gggaatttct cgaatgcctt gtttgatctt ttctgcggtt tcaaggattc tcttcgtcgc
     1501 agccagatac ccttcttcac caatggaagt caacgccgcc caacaagccg cgctcaaggc
     1561 gccgggacgg ctgccagcaa aggtcgggga aaaatacaaa ccgccaggcc attcagtggt
     1621 tgtgtaatat tgaaagcgcc gcaattcctg attgcgatag agaatgaccg aggtcccttt
     1681 ggcagcgtac ccatatttat gcgtatccac tgaaatggac gtcacacctg ccaggcgaaa
     1741 atcaaaggct gggacgtcat atcccaattt ttcagcccaa ggcaaaacaa aaccgccgag
     1801 gcaggcatcg gtgtggaaac caatgttgtg ctgacgcgcc aattcagaca tctctgcgat
     1861 cgggtcaatg gcgccatgcg gaaacgaagg tgccgaccct acaatgacga tcgtattctt
     1921 cgagatcgct tttttcactg ccttcaagtc agcgcgaaag ttttcatcca caggcacgtg
     1981 aatggtcttg atattaaagt actgactggc tttgtcgaaa gcagcgtgtg ccgttacagg
     2041 gacgatcatt tcaggccggg taatcccctt cacctcacgc gcccaatcac gataggtctt
     2101 catcgccagc atgatgcttt ccgtgccgcc tgaggaaagc gttccacagg catccacacc
     2161 tgccccgcca agcatggccg cagtcattga aacgatctct gcctcaaact tggtggcgct
     2221 tggccacaca tctgcatgga gcggattact ctgcgagtta agcgcataca cttgattcag
     2281 aaaatcaata tgcgcctgat caccgtgata gaccgcccca gagataaagc cgtccttcca
     2341 gtgagattcc tccagggcac gcatcgcttc catttcgttc aggatatcgg tgtgaggtcg
     2401 tcccttggca gggatcttgg aaaacgtggt gaatttgttt ttgtaaggct ttaccgaagt
     2461 ttccaactcg gtcatgatct cggcgtactc accttcgatc ttttttgcga cgctgggaat
     2521 attcttcatg cgtttttcca aaaacgccag cacccgcgga tttaatttcc ccgcctgctt
     2581 catcagtgta tccatccaac tcatattctt ctccagatga tggtagaggc acagcgttgc
     2641 tgtgcctcta ccatatagat taatgcattc gattcaaacg ggcatggatc ttgcgattgc
     2701 tttcgtagat cgcaaggaat tcacgaaaca actcatcata gatttttcga ttatcagggt
     2761 tgggcacata cgtggccgca attttcacat gtgtgccgat ctcatcgtat ttcaaataac
     2821 ccatccctgc agaagcaagc agggctgcgc cacgcacatt ggcttcgatc gggtccttca
     2881 cctgacggat ggggcgattc aacacatccg catggatctg gcaccagata ttcgattttg
     2941 caccgccccc caccatgttg atggattcga caggccgctt gttgaactgc tccacatact
     3001 gcaacagcca gcgcgcattg taagccacac cttcaaagac agcccgtacc atgtcggcgc
     3061 gggtggtgct gagtgactgg ttgtggaatc cgccgcgcac aagatgatct tccacaggag
     3121 tccgctcgcc atatagccag ggagtgaaga tcagttttcc actgcctgcc ggggtgcgtt
     3181 ctgccatttg gtcgagtaat tgataagcat tggcaggttt ctcgcccaca gacaattcat
     3241 cctgcggaaa caggatgttg ttccgcaggt attgcaagca tccaccagct gattcctgtt
     3301 cgttggtgag caaatatcgc ccagggattc ccgatggcag cgcggccagg ttatgaaaaa
     3361 tgtcggtctt tttgaagggg acatggcaaa ccaaccaacc tgatgtccca acgtataaat
     3421 gcggctcaaa atcacgtacg gcgccagaac caacagccgc cgagtgcaga tcaggcgaac
     3481 ccatgaatac ttttacgtca tcgcgcaggc cccatgcgct ggcgatctca cggcgcagag
     3541 tgcccaattg cgcattggca ggtttcaggt cgggaagttt cgcgcggtca atccccgcca
     3601 actggatcaa tccgtcatga tatttcacat tttgaatgtc gcgattgtcc gtcatccagt
     3661 gcagcgtgat cgagttgaac gatgcggcga attcgccagt aagcagcaga ccgatataat
     3721 ccatcggctc gaggaattta tatgtgcgtt gataaatatc ggggcggaca tctttgaggt
     3781 acaaaatatg cgcaagcgga tccttacccg agtttcccgg tgcgccgccc gtcaggcgaa
     3841 tccaattaag gaccttcgag agtccgtacc cttcaatttt cgggaaacca tctgcgatct
     3901 ttttgatata aggtgcgcca cgagaatcca tccagatgac ggcattttca atggcatttc
     3961 catctgcatc cactgcgacc gtgcccgacc attgtgtagt atttgcgatg gcaacaatgt
     4021 catcactgga aaccagcttc tttgcaagca gacgtttgca ggcggtatca attgcgctcc
     4081 accaaccagc aggtgactgt tccgcgccgc cattcggcaa aagaatgaca ggcgtttctt
     4141 gaaattcgct tccgatcaat tcaccttgcg tggaaaacaa tgcaaccttg ggacccgacg
     4201 tacccaggtc aatggcgaga atgaatttac ttgtggatga catttggcct cgagtttagg
     4261 agttgtgact gcatcaaaac acggcgatga tacaccaacc catgcgaaaa aaccataaat
     4321 cggtcaatcg tcatcgtcct ctttttgaac tccatacgcc cgagccactg cggcttcgat
     4381 ctcggcattg ctgtgggttt cacaggctac aagcgcccgt tgagggtgtt tcaccagcct
     4441 cccgcctgcg ctcattacct tggcatctac aaatttcccc ttcgaatcag tgaccacata
     4501 aagctggtac agtttcccac acatgataca gccatgtgat tcacctttgg tgaatgtctc
     4561 tgtcataaca aactcctttt cgttttcaaa acaaaacagg ccagagagaa ctccagcctg
     4621 ttcaatggtg acgctattga aaaagcagac tgtagtctgc gcttccagca attttattgc
     4681 gtaatgataa tactctcgat gacatcgcct tccacaccag ggttttgctg cgggtcgcgg
     4741 cgcgtgatgg cattcaccac atccataccg ctgaccactt caccaaaaat ggtgtgcaat
     4801 ccatttaacc aatctgtggg ggcataggta ataaagaact ggctgccatt ggtgttcggc
     4861 ccagaattgg ccatggccag cagcccttcc ctgtcaaacg tcaggtcgct aatttcatcc
     4921 tcaaaggaat acccaggccc gcccatgcct gagcctgtcg ggtcgccgcc ctgcgccata
     4981 aagccatcca ttacacgatg gaaagtcaca ccattgtaaa agcccttgca ggcaaggaac
     5041 acaaaattat tcactgtaac cggggcttta tcagaataca actgcacaac aaattcaccg
     5101 cccttctcca ttttgaaggt cgcaaaataa ggcttcgact catcaatgct caacggcggc
     5161 gcagatgtat attgttcggc agcggcaatg gaatcgaaaa cttcacaagc gggcgtggtg
     5221 ccgaccgcaa cattggattt tggcttcgga aggtacggcc acccaatgaa cccaagcacg
     5281 atcaaagcca aagccaccac cacatattga atggtccgat cagattttgc ctctctggcg
     5341 ttttttacat ttttcttttt tgccattcct gaactctcct caattgaatt tgttccaatt
     5401 atataacacc catggtgcaa caaccttgcg cacaggcgaa ttctcttgta tgcttgccaa
     5461 atatgcagca atacttcaaa aagcagatcg ccctcccgca agaccacgga tcatgggtgt
     5521 tcatcttcag cccactattg atcgggattt tcgcggggaa acatttcacc tacgccacct
     5581 tcaatctcat cattgcggcg atgtctgcat ttatgattcg ccaaccgatg acagtcatcg
     5641 tcaaaacgat ctcgggccgc cgcccaaaaa cggatcttcc cgccgcccgt ttctggttgc
     5701 ttgtctacgg gggaatcgcc accctggctt tactcggact tatccttgaa ggatttgctt
     5761 acattttata cctcgccatt ccaggcgccc ctgtctttgc gtggcacttg tggcttgtaa
     5821 gcaaacgttc cgagcgcaga caatcaggcg tggaaatcat tgcaacgggc gtccttgccc
     5881 ttgtggctcc cgcagccctt tgggtgggac ttggccatta tgacccagcg ggctggtggc
     5941 tctgggtttg ggcatggctt cagtctgccg cgagcatcgt ctacgcatat ctcaggcttg
     6001 agcagcggga acaggttgaa ggtcaggaaa gaagcgcact gtggaaggcg ggcagacggg
     6061 ccttcctgta cacatccttc aatttgatcc tgtcattgct gcttggctgg gcgggtttaa
     6121 ttccccaatt gatctttgcc gcattccttg tgcaatggct cgaaacgctt tggggcatca
     6181 cccaccctgc cacaggttgg aagccagtaa agatcggcat gcgtcaactc atcgtcagca
     6241 ttttgtggac agtccttttc atcgtcttca atcatgcctg accaaataac tcacaggaga
     6301 aaaaatggat tttgatctaa acgaagaaca aaaagcatgg aaaaaagccg tccacgattt
     6361 tgtgggcaaa gtggtcaaac cgaagtcacg tgaagtggat gagaccgagt cgtttaattg
     6421 ggaggcggtt cgcaagggag gtccgttggg gttgatgggc ctgagcatcc ctgaggcata
     6481 cggcggctca ggcgtggata tggtttcctc ttccattgca atggaagagt tgggctgggg
     6541 ctgtggttcc actgcattgg cgttcaccgc ccacaatggc ctcggcactg cgccgatcgc
     6601 cttgtatggc agcgaagaat tgaaaaagaa atggctgccg ctcgtggctg aaggcaaaaa
     6661 taaacttggc gcactcgcgc tgaccgagcc cggggcgggg tctgatattc aaggcggcgt
     6721 gatcacccgt gccgaaaaac agggcgatga atgggtcatc aacggcgcaa agatgtggtg
     6781 taccaatgcc agcatcgccg aatacatcat caccctcgta cgcaccgacc cgaaaggcgg
     6841 ctcaaaatca ttgagcatga tccttgtccc caccgattcc aagggactga ccattggccc
     6901 gcccgaaaag aaaatggggc tgcgcggatc acccgcctgc ggaatcagct acgacaatgt
     6961 acgcgtgccg cttggaaacc tcgttggtgc agaaggtcgt ggcctgcaac agactctggc
     7021 tacactcgac gcagggcgta tcagcatcgg tgccatttcc gttgggttgg cacaagccgc
     7081 gcttgaagcc gccgtggatt atgcccgtga gcgcaaagca tttggtcaaa gnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natgaccatc ggtgaaggca caagcgagat
     7261 ccaaagactg gtcatcgccc gccatgtatt ggcaagtgaa taacgtacat ttgaccgccg
     7321 actgcagtcg gcggtcaacg ttttgataca tctggaatca aactttattt tcggacaatt
     7381 acccaggcag tgccatcttc tgccctgatc ttcttttccc caatttcaaa caacttttct
     7441 attttatctg gcgcaaggaa gtgactccgc atcagcagca gcttttctgc aagcgcgatc
     7501 aacttcacgc caaaggtgcg gatatcaggc tcctcgatca ccagcacacc acccgtcttc
     7561 aacacacgga acatttcacg cgcactttca gcatggttga tgacatgatg caatgcatcc
     7621 accataatga cgcgttcaaa agattcgtcg gcaaagggca atgattcaga accgccgcac
     7681 acaggccgca gcggagtcgg aagcgtctct ttcaacatcc ccatggaaac atcggcgatc
     7741 accacttcat ccaccagatc acgaatggca gaagaaaccc gccccgtgcc gccgcccaca
     7801 tcaagcaggc gtcccttcac agggagcgca gccacctcac gcatgatctc acgttttgaa
     7861 taagtcaccc gcgcataaag cggggcaatc gctgcaaaat gatcgaatgg cattagacgg
     7921 tgatcaggtc tttgagacct tcaaaagtag ttgggccaat gcctgaaacg ttcatgatat
     7981 cttcgatctt tgcaaacggg ccattggcga gacggtaatc aatgattttc tgcgctgtgg
     8041 tcaaaccgat gccgggcaga gtatccagtt cttcaagggt ggcggtgttg atgttgatct
     8101 tctcacccgc gttcgggtcg ggtgttgcgg tttcagagcc tggcaattca acagtctcca
     8161 caggttcggc accatctttg tacggaatgc tcaactgctg gccatccaca accagtgcag
     8221 caagattcaa gccgtctaca tttgcatcgg ccagcaaacc gcctgcggca ttgatggcat
     8281 cctgaacacg ggcgccttcg ggcaggtcat acacatccgg gcgcggcacg gctccgatca
     8341 catgcacttt gatgggtgcc gccgtaggcg ccgcgcgcaa aacgatcggc tcaccagcag
     8401 gagcacgcga aacaaagatc aacgcacccg ccagcagaaa tccagccacg ataccaagca
     8461 gaatatataa aacgtttttc atgagacgat tttactccaa cttcatgacg agcaagtttt
     8521 cttccgtttt tcgaaacaac accgcaacat cgaatagatt cttgatgcgt ttgaaatatt
     8581 ttggcatttg gtcatcgggt actgtatgaa acccgaattt ttcatacatc gggccgttat
     8641 gggcaatgca cattaaatac acaggccgct cggtctgctc cagcaatacc tcgatcaccg
     8701 cacgcgccag cccttttcca cggtggtcgg gatgcaccgc aatcgaagcc agttcgcgga
     8761 tgtcggtccc gtgcggcttg atctgaccgc aggcaatgac ctgcccactg tcattcaccg
     8821 tcacgacaaa acgcttccaa tccaaccccg tcgggttgat tccgaccaaa ttgatcaggt
     8881 ccttaatttg ggcagattct gactccaacg caggacgaat cgattcttta ctcatttcac
     8941 aacgctcccg cagcaacaag aatgacaaac gtggcaatga accagtggat caggaacggc
     9001 cagacaaatg accttgtgcg ccaagccacc cagccaaagg caaatccgcc aaagatcgtg
     9061 gaaagtgttt ccacctcggg cttgctgata tgagccagcg caaacggaac cgcctgcaac
     9121 cacaaggctt catgcccgaa cctgcgcgca tacgcaaaca agatccaacc gcgaaagaaa
     9181 aactcccacc ccaaaaggtc cagaaaggtc gtccatggca gtccgggcag gtaatcggcg
     9241 taataggccc gcatcactgg gttaccatga cccaaataat aaatgatggg tgccatcaac
     9301 aaaatcccca aaatggtgta ggcaagcccc aacttccaat cgccaaagga aaagccatac
     9361 tcccttggat cttcaaaaaa gaaaatcgaa atcaccaaca ttggaatcgc aagatacagg
     9421 atgatacggt cccaatattt gtagggcgtc aatttgtgat acgaatccac catcaaaagc
     9481 aatgtggaga tgatcgtaac actgacaacc ctccaatcaa atttaagtct ttcgccagcc
     9541 agatacttca tttcctgatt ttttcaatac gttttgcaag gtaggaggaa aaataaccgt
     9601 cgtatttcac ccataattca ggcagtttcc aatcgttgcc tgttactttt ttgcggcaaa
     9661 gaggagagcc gcaataacaa tcgaattcat cgtacggccc gccatcagac atggcgtaat
     9721 caaagcacaa ttcttccccc gcgctgatat cacgcattgc caccaggccg atctgcccgg
     9781 tgaaacccag gttcggatca caggaatgat tgaagcaatc gtttggttcg ggggccgctg
     9841 atgtcaaaat gaaaaggtct tcctcgatct gcaaaacgcg ctgtgtaaaa cgcggcatgt
     9901 cagggtcgag ttttgagcga tggacaatgt caccgcccca caatgagatc aactcgcctt
     9961 tcgaaataaa gtcggtggca aaaacgccgc acccgccctc ggggtgctcg cgcgccttgc
    10021 attttcggtt gagaaaagaa aacgtcattt caactccaca ggaattttta ttgcatgaaa
    10081 aagatcaaag aaaaagagga atgccccttg tagaacaatt ccccatccgc tgccccgcca
    10141 aaatccatca accgaatttt gaatgagcca gacaccgccg aggatgtaca acacatccag
    10201 accgaagttg atccacaaca ctttgcggat gaaaatcgct tcacgcttcg ggtcggctgt
    10261 tgatttgcgc cgagcggcag ttcttgcgcc gacaatggca atcgctgcat cgatcaatcc
    10321 ccacacaacc gcttgaatgc caaagccgcg gccaaatcca tcggcggcga accagatgta
    10381 aagtccagaa aagatcgaga gaatgctcca gaaaagcagg cggctggtta ggtccgattc
    10441 aaatttccag atggggtgca gcatatgagg gattatacaa agaaaacgag cgacaaggag
    10501 aatcctcgcc gctcgcc
//
LOCUS       DAOQJC010000135        10313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_3377_length_10313_cov_9.125756, whole genome shotgun sequence.
ACCESSION   DAOQJC010000135 DAOQJC010000000
VERSION     DAOQJC010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10313
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3377_length_10313_cov_9.125756"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..2030
                     /locus_tag="PLE39_16105"
     CDS             138..2030
                     /locus_tag="PLE39_16105"
                     /inference="COORDINATES: protein motif:HMM:NF015083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HNE69907.1"
                     /translation="MNTLAKTFTTSLLVFLVGCQSLNPAPAQNTSNMLIEDAQANLLE
                     TTDESNSSRETESLDGIGNNTANPTWGSVNQPLLRFAAADYEDGNNSPAGADRPSPRL
                     ISNTFSVSPEEGIANDRDFTAFVYAWGQFLDHDIDLTDAASPKEAFPIVVPTGDAWFD
                     PANTGTMTIPVSRSNYDPNLSGPRQQTNSITAFIDGSQIYGVDPERAAALREFVGGRL
                     RTSTGNLPPLNTEGFPNANDAHRVDDSQLFLAGDVRANENPELLSLHTLFLREHNRIA
                     AEAAERHSDWTDEELYQFARRKVIAELQKITYDEFLPAILGPNAIPPYRGYQPEVNPG
                     IANEFSTAAFRFGHSMLGDDIEFLDNDGNENREAMPLRDSFFNPTVLHETDIDPILKY
                     LASDNAQEIDPIVVDDVRNFLFGAPGQGGFDLASLNIQRGRDHGLADYNTTRAAYGLE
                     RITSFEQITSDKALQASLASTYGSVDNIDLWIGGLAEDHLPNSSLGQTFTAILVDQFS
                     RLRDGDRFWYQASLSNRIVNDVQNTTLADIIRLNTDLTKLQADVFFFNESTVIVDAEA
                     PDQQNGQPNNGGSNGHQPPQAAINACSGLAENSSCSFIGRNQTTVNGTCLIVPSSTLA
                     CVPTRP"
     gene            2060..3937
                     /locus_tag="PLE39_16110"
     CDS             2060..3937
                     /locus_tag="PLE39_16110"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNE69908.1"
                     /translation="MKTRYIPLLLFALLFTALVPISNTQAATLAYTVVDTAQTKCYNA
                     TGEIACPSSGSSFYGQDAQTTGTAPSYTISADGLTVADNNTNLTWVRKPDTNNDNSLT
                     EADKVTYSGASSACNSKASANYGGYSDWRVPTIKELYSLMDFRGTDPSGLSGSDTSGL
                     TPFIDTDYFYFVYGDTSAGARIIDAQYWSSTAYIAPSNDNKVFGVNFADGRIKGYPST
                     MAKQFIRCVRGDSYGVNSFVNNGDGTVTDNSSGLMWSQSDSASAMNWQEALAWVQTKN
                     AENYLGHNDWRLPNAKELQSILDYSRSPDTSNSAAINAVFNATQITNEGGAADYPSYW
                     SNTTHATYNGSGGFAVYVSFGRALGWMQLQGQSCYTLVDVHGAGAQRSDPKSGSSSSY
                     YLGTSCNGGSAYGHGPQGDIIRVDNFVRLVRDAVTSSTTFTDVPETHELYPYIEALYQ
                     AGYTAGCSTSPMMFCPDQILDRAQSSVFMLRGAMGSAYTPPAAPWNTFVNDDWTGYEW
                     AQAWAEGMFQEGLTAGCQTSPLMFCPETQLSRVEASVFGLRMKYGVNYLPPTASGTLL
                     ADMTDTSYWGTAWAEQAYQDGLLPACGTQNGKPMFCPNDLLTRGWGAYLIVKAKNIPT
                     P"
     gene            4018..4755
                     /locus_tag="PLE39_16115"
     CDS             4018..4755
                     /locus_tag="PLE39_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69909.1"
                     /translation="MKRTSLFTLTTLLIVLTLAACRGAPPPPDQTTNTQPVPPTSDGS
                     VNTASMVNIKADVWADNWFAFYLADQLIKEDSVSITTERSFNAETFTFAASYPMTFNF
                     IAKDFKENDTGLEYIGTNRQQMGDGGLIAQFINADTGEVIAVTDSNWMCTVIHEAPLD
                     KACETSANPVAGEMPCGFTSLEEPANWMDAAFDTSAWAKATIYSAGQVDPKDGFEQIA
                     WNPSAQFIWGPDLETNNTLLCKLTING"
     gene            4759..5334
                     /locus_tag="PLE39_16120"
     CDS             4759..5334
                     /locus_tag="PLE39_16120"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNE69910.1"
                     /translation="MKTNIFSLFLIFLLMGCSLAPGDPPAPPDTTPVIDPVSSNGTFL
                     LTSPEVSEGGALPREFTCDGSSATLPLAWSGAPTGTKSFAVVMHHIPGPGDSHWYWVV
                     YNIPADILNLEKNSTGIGTLGTNSVNGQTEYAPPCSKGPGEKVYTYTVYALSAEPQIS
                     LSASQVSREVLLDAIQNITLASAALNVTYTR"
     gene            5338..6306
                     /locus_tag="PLE39_16125"
     CDS             5338..6306
                     /locus_tag="PLE39_16125"
                     /inference="COORDINATES: protein motif:HMM:NF025600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HNE69911.1"
                     /translation="MSITRRDFLRLSAYGFGTAFLAACGFRPGDNPPPLNPTELPALS
                     PTVTSQALKELGFEAFADQLKVYQDAQYLYVESDGMPNHPMMIGIKSWQQQVPLPQPF
                     TGSNAWKIPLHPVVAENPISAKTDLYRGAIALAVNGVPIFNALNNRGDDAYLYGELDE
                     FGGHAGRADDYHYHTAPLHLSELVGVDKPIAYALDGFPIYGLTEPDGSVVTDLDEFNG
                     HFAADGSYHYHGTKTYPYINGGMRGLVTVRDDQIEPQPHMTPIREALQPLQGATITNF
                     TELDSSHYSLEYQINGQKYYVNYGFEGNTYTFEFVDASGKKTVETY"
     gene            complement(6323..7990)
                     /locus_tag="PLE39_16130"
     CDS             complement(6323..7990)
                     /locus_tag="PLE39_16130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE69912.1"
                     /translation="MNLRKTFLVLLLLALTIAPRLSALDSFVTIDEPFWLSVGANYYY
                     ALGQRELENTVYEYHPAVTTMSVITAAFLVDFPDYRGLGQGYFDVDKEKFDPFIVANG
                     HDPMVLLVISRLFQVAIIILFVLIIFYLLSVLFGEEKAFFITAFVSSAPYFLGHSRVL
                     SHEAMVAFFVLASIFGLLAYLEFGRKGHYLLISGVSAALAQLTKSSAMAMFPVIVLML
                     AMFIKERGLKSALRDFGIWLGWLALAYFIFWPGMWVAPGKMLYEVYGNAFSYALQGAR
                     LQVTQELQPSRFSLAAMGGSILNFILQVATKETPLSWIGIGLALFSLRRAGAAFEKRL
                     MLYLFLNAVMSILLFSAAQGRNSPHYIMSAHVSMDVIAALGWVSLSGLTLNQNARTNF
                     QIKAGSVVFLLVMQLGSALFYHPYYYTYHNPLVVNAPLSDYGEGFEQAAAYLAQKPNA
                     ESLKVLSFRGRGPFSYFFSGETIIFNPLFLEEPGMVSMIERLEQADYLVINDALRPRT
                     PRTELFVSALDGVTPEHSIFINGVSTIRIYRMADLPASFYEVLSNAR"
     gene            complement(8034..9794)
                     /locus_tag="PLE39_16135"
     CDS             complement(8034..9794)
                     /locus_tag="PLE39_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69913.1"
                     /translation="MKNSIRILIAVIVIAAMILPRAANLTQFTAADEPFWLVVGANYY
                     YALTHGELENTVYEYHPAVTTMWIGTAAMLIYFPEYRGLMDGYFQQEKTTFDDYLVEH
                     GKDPLALLWWARLIQLLLNVALFIIAFFFLKSLLDENSALIILLLTSFAPYLFGQSRM
                     LNHESMVGLFSILTILAMTAHLFLNPKTGLVILSAGFAAFANLTKSSAIVLFPVVAVM
                     IVVHVMSLRGGAERRRSNPQLNGEIAHLPLRAVQGSGEKQVRPRNDIINGLKTYTLWI
                     IAFFIVYFIAWPGMWVAPGKMLYEVYGNAFSYAFQGARLSVTQELQPSNFSLASAGLG
                     VFDFLTSIAWRTTPITWIGLALSIIFFFSKKEEVVSPLAKYLVASLLLEALACIGMFS
                     LVQGRDQPHYVLASYYGFEFAAAIGWALALRWLSLSFAWAGQRSAQIAFVAILLSAQA
                     FPLLQNAPYFFTYLNPIMTAAAGKPAPFFYGERMEQAAAYLAQQPDAKDQTALVYFGR
                     SFSYYYPGKTLLFKPILFDDKAQLIENLRESDYLVMYSGLEDRLPLLKELTPEHVIEL
                     YGRTYVEIYRVADIPASFYK"
     gene            9894..>10313
                     /locus_tag="PLE39_16140"
     CDS             9894..>10313
                     /locus_tag="PLE39_16140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNE69914.1"
                     /translation="MNTSSNENQFHKDLIDNLYDGVYFVDENRLITYWNKGAERITGY
                     HAEDVLGKFCHANILDHVDDDGKHLCMDGCPLLATIQDGQPRESEMHLRHASGHRVAV
                     QVRCSPIINAQNEIVGAVEVFSNNQSLFKMRRRVDELE"
BASE COUNT         2712 a         3081 c         2474 g         2046 t
ORIGIN      
        1 agtgcaggtg aggcacaaag tctcgaagac tcaaagaaaa aaagcttggt gccttggtga
       61 ctttgtggtc tacaacactt tacttatgca agaggtcaat tcaatctaaa aatagttcaa
      121 caccaaagga acccaacatg aacaccctcg ccaaaacctt taccaccagc ctgctcgtct
      181 tcctcgtcgg gtgccagtct ctcaaccctg cccccgctca aaacacctcc aacatgctga
      241 tcgaagatgc acaggcaaac ttattggaaa caaccgacga atccaattcc agccgcgaaa
      301 ccgaatcgct ggatggaatc ggaaacaaca ccgccaaccc aacctgggga agcgtcaacc
      361 agcccctgct tcgcttcgcc gcagcggact atgaagatgg aaacaactcc cccgcaggcg
      421 cggatcgtcc cagcccgagg ctgatcagca acacattctc ggtcagcccc gaagaaggca
      481 tcgccaatga ccgcgatttc accgccttcg tctacgcttg gggacaattc ctcgaccacg
      541 atattgacct gaccgacgcc gcctcgccga aagaggcttt ccccattgtc gttcccacag
      601 gcgatgcatg gttcgacccc gccaacacag gtacgatgac aatccccgtc tcacgctcga
      661 actacgaccc aaacctcagc ggcccgcgcc agcagaccaa ttccatcacc gcattcattg
      721 atggctccca aatttatggc gtagaccccg aacgtgcggc ggccttgcgc gaattcgtcg
      781 gcggacgact gaggaccagc acaggcaacc tgccgccact caacaccgaa ggcttcccca
      841 atgccaacga tgcccaccgt gtggatgaca gccagctgtt tcttgcaggc gatgtccgcg
      901 ccaacgaaaa ccccgaactg ctttccctgc acacgctctt cctgcgcgag cacaaccgca
      961 ttgccgcaga agccgccgaa cgccactccg attggaccga cgaggaactg tatcaatttg
     1021 cgcgccgcaa ggtcattgcc gaattgcaaa agatcaccta cgacgaattc ctccccgcca
     1081 tccttggtcc caatgccata cccccctacc gcggctacca accagaagtg aaccccggca
     1141 tcgccaatga attttccacc gcggcctttc gttttggtca cagcatgttg ggcgatgaca
     1201 tcgaatttct cgacaatgac gggaacgaaa atcgcgaagc catgccactg cgcgattcct
     1261 tcttcaaccc cacggtgctc cacgaaacag atattgaccc cattctgaaa tatctcgcct
     1321 ctgacaacgc ccaggaaatt gaccccatcg tcgtggatga cgtccgcaat ttcctctttg
     1381 gcgcccccgg tcaaggcgga ttcgacctcg cctccctcaa catccaacgt ggacgcgacc
     1441 acggactcgc cgactacaac accacccgtg ccgcctacgg cctcgaacgc atcaccagct
     1501 tcgagcaaat cacctcagac aaagccctgc aagcctccct cgcctccacc tacggcagcg
     1561 tggacaacat tgacctgtgg atcggcggcc tcgccgaaga ccacctgccc aactccagcc
     1621 tcggccaaac cttcaccgca atcctcgtcg atcagttcag ccgtctgcgt gatggtgacc
     1681 gtttctggta tcaggcttcc ctgtcgaatc gaatcgtcaa cgatgtgcaa aacaccaccc
     1741 ttgccgatat catccgcctc aacaccgacc tgaccaaact tcaggcagac gtcttcttct
     1801 tcaacgaatc caccgtcatt gtggatgctg aagcgcccga tcagcaaaac ggacaaccaa
     1861 acaacggcgg atccaacgga caccagcctc cgcaagccgc gatcaacgcc tgctcaggcc
     1921 tcgctgaaaa ctcctcctgc tctttcatcg gcaggaatca aaccaccgtc aacggtacct
     1981 gtctcatcgt tccaagctca acgcttgctt gtgtccccac aaggccatag cacaaagaga
     2041 atcatcaaag gagaatgaaa tgaaaacccg ctacattcct ctgcttctat ttgccctgct
     2101 gttcaccgcg ctcgttccca tttccaacac acaggcggca acccttgcct acacagtggt
     2161 ggataccgcc caaaccaaat gctacaacgc gactggagaa atcgcctgcc catcaagcgg
     2221 atcctccttc tacggtcagg acgcacagac aacgggtacg gccccgagct acaccatcag
     2281 cgctgacggt ttgaccgtgg ccgacaacaa cacaaacctg acctgggtcc gcaagcccga
     2341 taccaacaac gacaactccc tcaccgaagc ggacaaggtg acctattcag gcgcatcctc
     2401 ggcctgcaac agcaaagcat ctgccaacta cggcggctac agtgactggc gcgtgcccac
     2461 catcaaagaa ctgtactcgc tgatggattt ccgcggtacc gaccccagcg ggctctcagg
     2521 ctccgacact tcaggcctta ccccgttcat tgacaccgat tatttctatt tcgtctacgg
     2581 cgatacgtcg gcgggtgcgc gcatcatcga cgcccaatac tggtcgagca cggcttatat
     2641 cgcaccatcc aacgacaaca aggtcttcgg cgtcaacttt gccgatggac gcatcaaagg
     2701 ctatcccagc acgatggcaa agcaattcat ccgctgtgtg cgcggcgaca gctacggagt
     2761 caacagtttc gtgaacaacg gcgatggaac ggtcaccgat aattccagcg gactgatgtg
     2821 gtcacaaagc gacagtgcca gcgccatgaa ctggcaggaa gccctggcct gggtgcagac
     2881 gaagaatgct gaaaattatc tcggtcacaa cgactggcgc ctgcccaatg ccaaggaact
     2941 gcaaagcatc ctcgattaca gccgctcacc cgatacaagc aactccgccg cgatcaatgc
     3001 tgttttcaac gccacacaga tcaccaacga aggcggcgcc gcagactatc cgtcctactg
     3061 gtccaacacc acccatgcca cctacaacgg cagcggcgga ttcgcggtct acgtttcctt
     3121 tggccgcgcc ctcggctgga tgcaattgca aggacaatcc tgctacaccc ttgtagatgt
     3181 tcacggtgca ggcgcacaac gcagcgaccc gaagagcggc agttcatcaa gctattatct
     3241 cggcacaagc tgcaatggcg gctctgctta cgggcacgga ccgcaaggtg acatcatccg
     3301 cgtagataat tttgtccgtc tcgtgcgcga tgcagtgacc tccagcacca ccttcaccga
     3361 tgttcctgaa acgcatgaac tctatcccta catcgaagcc ttgtatcagg caggctacac
     3421 cgcaggctgc agcaccagcc ccatgatgtt ctgccccgac caaattctcg accgtgctca
     3481 atcttccgtg ttcatgttgc gtggcgcaat gggctctgcc tacaccccgc ccgccgcccc
     3541 gtggaatacc tttgtcaacg acgattggac gggttatgaa tgggcgcaag cctgggccga
     3601 aggcatgttt caggaaggac tcacagcagg ctgccagacg agtccgctca tgttctgccc
     3661 tgaaacccaa ctctcccgtg tggaagccag tgtcttcggc ttgcggatga aatatggcgt
     3721 caactacctg ccccctacgg ctagtggaac cctgctcgcc gatatgaccg acaccagtta
     3781 ttggggtacc gcctgggccg aacaggccta tcaagatggc ttgctccccg cctgtggaac
     3841 ccaaaacggc aaacccatgt tctgcccaaa tgacctcctc acccgcggat ggggcgccta
     3901 tttgatcgtc aaagccaaaa acattccaac cccataaaat catatacttt ggctacgctc
     3961 gcattgaaat ctatgcgagt gtagccaaag aaaaccaact caaaaggact ctccatcatg
     4021 aaacgaacat cgcttttcac attgaccacc ctgctgatcg tgctcacact ggccgcctgt
     4081 cgcggcgcac cgccaccccc cgaccaaacg accaacacac agccagtccc acccacgtca
     4141 gatggatccg tcaacacggc gagcatggtt aacatcaaag ccgatgtctg ggctgacaat
     4201 tggtttgctt tctatctggc tgaccaactc attaaagaag attcagtttc aattacgacc
     4261 gaacgctcct tcaatgcaga gactttcacc tttgccgcat cctacccgat gacctttaat
     4321 ttcatcgcca aagatttcaa agaaaatgac acaggtctcg aatacatcgg caccaatcgc
     4381 cagcaaatgg gcgatggcgg tttgatcgcg caattcatca acgccgacac aggcgaggtg
     4441 atcgccgtca ccgattcaaa ctggatgtgt accgtcatcc acgaagcgcc gctggataag
     4501 gcctgcgaaa catccgccaa ccctgtggca ggcgaaatgc cctgtggatt cacttctttg
     4561 gaagaacctg caaactggat ggatgccgcc ttcgacacgt ctgcctgggc caaagccact
     4621 atctattccg ctgggcaggt tgacccgaag gatggattcg agcagatcgc gtggaatcca
     4681 tctgcgcaat tcatctgggg tcccgacctt gaaacgaaca atacgcttct gtgcaaattg
     4741 acgatcaatg gatgacgcat gaaaacaaat atattttcgt tatttctcat cttcctgctg
     4801 atgggttgct cgctcgcccc cggtgacccg cctgctccgc ccgacacaac tccagtgatc
     4861 gaccctgtct catcaaatgg cacattcctg ctcaccagtc cagaagtatc cgaaggcggt
     4921 gccctgccca gggaattcac ctgcgatggt tcaagcgcca ccctgcccct tgcgtggagc
     4981 ggagcaccca cagggacaaa aagtttcgct gtcgtcatgc atcacatccc cggacctggt
     5041 gatagtcatt ggtattgggt tgtttacaac attcccgccg atattctgaa tcttgaaaag
     5101 aacagcacgg gcattggcac actcggaacc aacagtgtca acggccaaac cgaatacgcg
     5161 ccaccctgct caaaaggccc tggcgaaaaa gtttacacct acaccgtcta cgcgctttcc
     5221 gccgaaccac agatttctct ctccgcttca caggtcagcc gcgaggtatt gctcgatgcg
     5281 attcaaaata tcaccctcgc cagcgctgct ttgaatgtaa cctacacgag gtaactcatg
     5341 tccatcacgc gccgcgattt tctaagactc tccgcctacg gattcggcac cgccttcctc
     5401 gccgcctgcg gattcagacc cggggacaat ccccctccgc tcaacccaac cgaactgcct
     5461 gccctgtcgc caacggtcac ttcacaagcc ttgaaagaac tcggcttcga agccttcgcc
     5521 gatcaattga aggtgtatca ggatgcccaa tatctttatg tcgaatccga tggaatgccc
     5581 aaccacccaa tgatgatcgg aatcaaaagc tggcagcaac aggtgccgct gccccaaccc
     5641 ttcactggaa gcaacgcatg gaaaattccc ctccaccccg ttgttgcaga gaatcccatc
     5701 tcggcaaaga ccgacctgta tcgcggcgcc attgccctcg ctgtgaacgg cgtcccaatt
     5761 ttcaacgcgc tcaacaatcg cggcgacgat gcctacctct atggtgaatt ggatgaattc
     5821 ggcggtcacg caggccgcgc cgacgattat cactatcaca ccgccccact gcatctgtcc
     5881 gaacttgtcg gcgtggataa acccatcgcc tacgccctag atggctttcc catctacggc
     5941 ctcaccgaac ccgatggctc cgtcgtcacc gacctcgacg aattcaacgg tcacttcgcc
     6001 gcagacggca gttatcacta tcacggcacg aagacctatc cctacatcaa cggcggcatg
     6061 cgcggattgg tcaccgtccg cgatgaccag atcgaacccc agccgcatat gaccccgatc
     6121 cgcgaggcgc tgcaacccct gcaaggtgcc accatcacca acttcaccga actggattcc
     6181 agccactatt cgctggaata ccagatcaac gggcaaaagt attacgtcaa ttatgggttc
     6241 gagggaaata cctacacgtt cgaatttgtg gatgcttcag gaaagaaaac cgtggagacc
     6301 tattaaaccg taacgcgaga gattatctcg cgttacttaa tacttcataa aacgatgctg
     6361 gcaggtcagc catgcgataa attcggatcg tcgaaacccc gttgatgaaa atcgaatgct
     6421 cgggcgtcac accgtccaag gcgcttacga ataattccgt tcgtggtgta cgcggcctca
     6481 acgcatcatt gatcaccaga taatccgcct gctccaaccg ctcgatcatc gaaaccatgc
     6541 caggctcctc caaaaacagc ggattgaaaa tgatggtctc ccccgaaaag aaataagaaa
     6601 atggtccgcg tccgcgaaac gacagcacct tcaacgactc tgcattcggc ttctgtgcca
     6661 aatacgccgc agcctgctca aagccttcgc cataatctga aagcggcgca ttcaccacaa
     6721 gcggattatg gtacgtgtaa taatacggat gataaaacag cgcactgccc aactgcatca
     6781 cgaggaggaa gaccacgctt cctgccttaa tctgaaaatt ggttcgggca ttctgattca
     6841 gggtcaaccc tgaaagagag acccaaccca gcgccgcaat cacatccatg ctcacatgcg
     6901 ccgacatgat gtagtgcggc gaattccgtc cctgcgccgc gctgaacagc aaaatggaca
     6961 tgaccgcatt cagaaaaaga taaagcatga gtcttttctc gaaggccgca cccgctctcc
     7021 gcaaggaaaa gagcgctaaa ccgatcccga tccatgaaag cggtgtctct ttcgtcgcca
     7081 cctgcaaaat gaaattcaag atcgatccgc ccattgccgc aaggctgaaa cgcgaaggct
     7141 gcaattcctg ggtcacttgc aggcgtgcgc cttgcaaggc ataactgaag gcattgccat
     7201 atacctcgta aagcattttg cccggtgcca cccacatacc gggccaaaaa ataaaatacg
     7261 ccagggccag ccagcccaac caaatgccga aatcccgtaa cgcggactta agtccgcgct
     7321 ccttaatgaa cattgccagc atcaacacaa tgaccgggaa cattgccatc gctgaagatt
     7381 ttgtcagctg tgccagcgcg gcagaaacac ccgagatcaa aaggtaatgt cccttgcgcc
     7441 cgaattccag ataggccagc aacccaaaga tcgaagccag tacaaagaac gccaccatcg
     7501 cttcatggct caacacccgc gaatgcccca gaaaatatgg cgcactggaa acaaaggccg
     7561 tgataaaaaa cgccttttcc tccccgaaca aaaccgaaag cagataaaaa atgatcaaca
     7621 cgaataaaat gatgatcgcc acctgaaaga ggcggctgat gaccagcaaa accatcgggt
     7681 catgtccatt ggcaacgatg aacggatcga acttctcttt atccacatcg aaatatccct
     7741 gccccaaccc gcggtaatcg gggaaatcca ccaggaaggc ggccgtgatg accgacatcg
     7801 tcgtcacagc ggggtgatat tcatacaccg tattttccaa ctcacgctga ccgagggcat
     7861 agtaataatt cgcacccacc gagagccaga aaggctcgtc aatggtgacg aaagaatcca
     7921 gcgccgaaag acgcggggca atggtcaaag caagcaggag gaggacaagg aatgttttac
     7981 ggaggttcat gggttgtgtc cgtcataagt gacagtcacc tttgaggtga ctgtcacttg
     8041 taaaaactcg cgggaatatc cgctacgcga tagatctcca cgtaggttcg gccatacaac
     8101 tcgatgacat gttcaggagt caactctttg agcagcggca ggcggtcttc gaggcctgag
     8161 tacatgacga ggtaatctga ttcgcgcaaa ttctcgatca attgggcttt gtcatcgaaa
     8221 aggattggtt tgaatagcag ggtcttcccg ggatagtaat agctgaaact tcggccaaaa
     8281 taaacgaggg cggtctggtc tttggcatcg ggttgttgcg cgaggtaggc ggcggcctgt
     8341 tccatgcgtt cgccatagaa aaagggagca ggctttccag ccgcagccgt catgatcgga
     8401 ttcaggtagg tgaaaaaata cggtgcattt tgcaatagcg gaaaagcctg tgcgctcaag
     8461 agaatcgcaa caaaagcgat ctgcgctgat ctctggcctg cccatgcaaa agacaggctg
     8521 agccagcgca aagccaatgc ccagccgatt gcagcggcaa actcgaaacc ataataggaa
     8581 gcaaggacat aatgcggctg gtcacgtccc tgcacgaggg agaacatgcc aatgcaggca
     8641 agggcttcga gaagcaggga ggcaaccaga tatttggcaa gcggagaaac aacctcctct
     8701 tttttggaaa agaaaaaaat aattgaaagt gcaagtccga tccatgtgat gggggtggtg
     8761 cgccatgcaa tggaagtgag gaagtcaaaa acgccgagcc ctgccgaggc aaggctgaaa
     8821 ttggaaggct gtagttcttg tgtaacggag agccgcgctc cctgaaaggc atagctaaag
     8881 gcatttccgt acacttcgta gagcatcttg cccggtgcca cccacatccc cggccacgcg
     8941 atgaagtaga caatgaagaa ggcgataatc cacagggtgt aggttttgag tccgttgata
     9001 atgtcattgc gagggcgcac ttgtttttcg cccgaccctt gcaccgcccg caagggcagg
     9061 tgagcaatct ccccgttaag ctgggggttg cttcgtcgtc gctccgctcc tcctcgcaac
     9121 gacatgacgt ggacgacaat catcaccgca accacgggga acaatacgat cgccgaggac
     9181 ttggtgaggt tggcaaaggc ggcgaatcct gccgatagaa tcacaagacc agttttcgga
     9241 ttaagaaaga gatgcgctgt catcgcgagg atggtgagta tggagaagag tcccaccatg
     9301 gattcgtggt tcaacatccg cgattggccg aaaaggtagg gtgcaaatga agttagcagg
     9361 agaatgatga gggctgaatt ttcatcgagc agggatttca agaagaagaa ggcgatgata
     9421 aaaagtgcaa cattgagtag aagttgaatc agccgcgccc accataacaa cgcaagcggg
     9481 tctttgccat gctcaacaag gtagtcatcg aaggtggttt tttcctgctg gaagtagcca
     9541 tccattaagc cgcgatattc gggaaagtag atcagcatgg cggcggtgcc gatccacatg
     9601 gtggtgacgg cggggtgata ttcgtagacg gtgttttcga gttcaccgtg tgtaagggca
     9661 taataataat tcgcgccgac cacgagccaa aaaggttcat cggcggcggt gaattgggtg
     9721 agattcgccg cgcgcggaag gatcatggct gcaataacaa tgacggcgat caaaatgcga
     9781 atggagtttt tcatggcgaa aggattatac caagcgaaaa aaaacggggg cgaaatgaaa
     9841 aacgaaatca gcctgtaaga cacaaatgca ttccaatatg ataatattta ttcatgaaca
     9901 catcatccaa tgaaaatcag tttcacaaag acctgattga caatctatat gatggggttt
     9961 atttcgtaga tgaaaacagg ctgattacgt actggaacaa aggcgcagaa cgcatcaccg
    10021 gctatcacgc agaagatgtg ctgggaaagt tttgccacgc caatattctg gatcatgttg
    10081 acgatgacgg caaacacctg tgcatggacg ggtgtccgct gctggcaacc attcaagatg
    10141 gtcaaccgcg cgaatcggaa atgcacctgc gtcatgcaag cggacatcgt gtggcagtgc
    10201 aggtgagatg ctcccccatc atcaacgcgc aaaatgaaat tgtgggcgca gtagaggttt
    10261 tcagcaacaa ccaatccctt ttcaagatgc ggcgcagggt ggatgaactg gaa
//
LOCUS       DAOQJC010000136        10204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_3428_length_10204_cov_11.554340, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000136 DAOQJC010000000
VERSION     DAOQJC010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10204
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3428_length_10204_cov_11.554340"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLE39_16145"
     CDS             complement(<1..410)
                     /locus_tag="PLE39_16145"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNE69915.1"
                     /translation="MSDYKFFHPTEVRYGDLDPQGHVNNAKYLTYFEQARIYYLIKLG
                     LFNKDQSFMEVGVIIADIHITYHSTTHYGDDIKVGVKITRIGTKSITVEQCVMDAKTG
                     KLMASGTVVMVTFDYEGLKTIPVPEEWKQKISAF"
     gene            complement(512..1210)
                     /locus_tag="PLE39_16150"
     CDS             complement(512..1210)
                     /locus_tag="PLE39_16150"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE69916.1"
                     /translation="MTEIKRDDTRNILSDIKLFEGLSSAQLDWVAKHAHRRIFTAGTN
                     VLTIEQPGEAVFIILHGTVKIHIEQGERDVIISVLGSGDILGEMSLIDSVGRSASAIT
                     LENSLMLWMDKTTFYHILDNFPPVARNLVKILTARVRLSDQVIQSLATLDVNGRVARQ
                     LLAFAERYGHEIADGVQVHIVLTQGDLADLVGASRKRVNQAMVFFKEQGLVSTDADGR
                     FVIHNKAGLMHFCN"
     gene            complement(1233..2195)
                     /locus_tag="PLE39_16155"
     CDS             complement(1233..2195)
                     /locus_tag="PLE39_16155"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE69917.1"
                     /translation="MNDELKIKFWGVRGSYPTPGADTIRYGGNTSCVEVSAGGRTIIL
                     DAGTGIIPLGRELAGQKRPLELLLLFSHLHHDHTQGFPFFIPAYIPNTRLHIFGPGGT
                     PETVKHVLEHNQSAETFPLSLRDMAAAKQIESVRESQVIVWDEENVRVVGSASGLSPE
                     AVVIRIHRSYAHPGGVYVYRISWRGKSVVYATDTEGYVGMDRKLAAFARDADVLIHDA
                     QYSEEHYRGMLAGFPSTQGYGHSTALMAAEVAASAETGELILFHHDPAYSDDWVAAQE
                     AAAKKLFPDSCAAYEGLEIRLKPEQAGMVLTGPTQARRGNVSPR"
     gene            2377..2589
                     /locus_tag="PLE39_16160"
     CDS             2377..2589
                     /locus_tag="PLE39_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HNE69918.1"
                     /translation="MLPDLQLKDHLRLRKPHPCGSYEWTVVRLGADIGLECRGCQHRV
                     MLTRRELAKRLKTNLTKHEEKKEEGK"
     gene            2686..3924
                     /locus_tag="PLE39_16165"
     CDS             2686..3924
                     /locus_tag="PLE39_16165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE69919.1"
                     /translation="MAETEARSVPKVGSLRIGLFTDTYAPQVNGVSISLQLISDGLRR
                     RGHQVTIFAPRFPGYEDNETNVMRLPSLKYLNNPPIYVAVLGTPRSTWSLTRKHFDVL
                     HAHSPASVGLLAYLTASTKRLPLIYTYHTSITDYTHYVKFIGGSSIIKHAASWFSTTS
                     TNLGDQIVVPSPKFHRLLLEQKVKRPIHIIPNGINLDGFKSAKNPGSFRNRLGMQPDA
                     PILLSVGRIDPEKRLDFLIDAFDRIADHVPNARLVFAGDGSARKKLEEHAASVRSKDR
                     IHFLGMVNRADLPDLLHDAAVFLSASTTEVHPISVIEAIAAGLPVLAVQDEAFEGMVI
                     DGENGHQTLLDVNVYSDTLLSLLKDPERLSRYGKRSAELSEKYSIEGQVRALENLYIE
                     SILQNWRGSLVSRISARLKL"
     gene            4051..4548
                     /gene="tsaE"
                     /locus_tag="PLE39_16170"
     CDS             4051..4548
                     /gene="tsaE"
                     /locus_tag="PLE39_16170"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNE69920.1"
                     /translation="MPILDSHTIEFFSRSPEQTRRIGMRTGAGLKAGDVICLQGNLGA
                     GKTTFVQGLAQGWGSLDSVSSPTFILVNQYRRANGDRLFHLDAYRIESMPEGEELDLD
                     SMLADGALVIEWPERLGDLIPKERMWINLEHMAEEHREMRFNANGKRYDDLLDEIRQS
                     IFGGA"
     gene            4548..5219
                     /gene="tsaB"
                     /locus_tag="PLE39_16175"
     CDS             4548..5219
                     /gene="tsaB"
                     /locus_tag="PLE39_16175"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNE69921.1"
                     /translation="MLIAVDTSTAQVGLALYDGNQVLGELTWTTRQHHTTELAPALTG
                     LLSRSGVSMDMVSALGVAIGPGSFTSLRVGLALVKGLALARRLPVIGISTLDVIAAAQ
                     PASKHPLLAVIQAGRKRIAFSEYKSLKNEWQVQGPVRSGTVDELADSIESPTVVAGEF
                     SPDDRSRLSKKRAKILLASPAYCVRRPSLLAELAWERWQKNEVDDAASLAPIYLHVEG
                     TPIPS"
     gene            5240..5758
                     /gene="rimI"
                     /locus_tag="PLE39_16180"
     CDS             5240..5758
                     /gene="rimI"
                     /locus_tag="PLE39_16180"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNE69922.1"
                     /translation="MNVLSPDDGSGLMKIRKMTVDDLEQVIAIDQVSFGLPWPPRSFS
                     FELTDNPPSRCWVVEMNGRVVAMLVAWLIVDEIHIATFATHPDHRRQGIGGRLLYHTL
                     RTAREEGAITSFLEVRAGNESALALYRKFGYVESGLRKGYYSDNHEDAVLMTLGPADY
                     ERLLLHGEADER"
     gene            5748..6392
                     /locus_tag="PLE39_16185"
     CDS             5748..6392
                     /locus_tag="PLE39_16185"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNE69923.1"
                     /translation="MSAEETLAQLAVKISTCTNCALHESRKKAVTGDGPADAEIMFIG
                     EGPGFHENEQGHPFVGASGKFLDQLLAQAGVTRAEVFIANVVKCRPPGNRDPLPEELA
                     ACDEYLEAQIQTINPSIIVTLGRFSMNKFIPGAKISAIHGQMRKVGDRFVIPMFHPAA
                     ALHQAALKPSILADFANLPDQLNEARAALGRKVVAQKKEAAAPKNEKPRQLSLF"
     gene            6433..6882
                     /locus_tag="PLE39_16190"
     CDS             6433..6882
                     /locus_tag="PLE39_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69924.1"
                     /translation="MPEEINATQKPWYERFLEYVIGLLLLASLTYGISHSSFRRKIDK
                     DEIAQKVLENCKYHSFMYMQEEPLQANAELMTCSEVQIKTEMNECDNRNPDMKVWFVA
                     TDGLWFLYGPVVGNGDLPPPILINSCHALVDARNGMILETGYSKFKK"
     gene            6936..8258
                     /locus_tag="PLE39_16195"
     CDS             6936..8258
                     /locus_tag="PLE39_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNE69925.1"
                     /translation="MSTKETLIKKLKEKNAKIAILGLGYVGLPLAVVFGEAGFNVTGV
                     DPDTRKIEALNKGVSYIPDVKTEAVAKLVKDGKFTATADFSVLKEMDAVSICVPTPLR
                     QTGDPDMSFIISATEQLAKYMHKGMVVVLESTTYPGTTRELLLPKLGVAHDLKIGEDW
                     FLAFSPERVDPGREDFTTINTPKVMGGITEACGEVATAWYEGAIKIVHHVSTAEAAEM
                     SKLLENTFRMINIGLVNELAIMCERLGVDVWEVIDAAATKPFGFMKFTPGPGLGGHCI
                     PIDPLYLSWKMKSFNYNARFIELASEINTNMPRYVVARILDAMNDRGMILKGSKVLVL
                     GVAYKPDIDDVRESPALDVIGLLQKKGAVVEYHDPYIPHIHHEYDGWHMDSISDVMKS
                     VQSADAVVVVTNHKSYDYKAIVDAAKFVFDTRNATSKVAKNSENVVRL"
     gene            8267..9247
                     /locus_tag="PLE39_16200"
     CDS             8267..9247
                     /locus_tag="PLE39_16200"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNE69926.1"
                     /translation="MGHYLVTGAAGFIGARTSELLIGQGHTVVGIDNMNDAYDPRMKE
                     YRLKKLQAMKGFEFVRDDISDKGILKRQAALVSRPFDGVINLAARAGVRFSVDNPWVF
                     LESNVLGTLNMLEICRQYGCKKFILASTSSIYGENPPYPTPETASSSEPMQPYAASKK
                     GAEALAHSYHHLHDIDVTIVRYFTVYGPAGRPDLAMFRFVQWILEGQPIRINGDGNQS
                     RGFTYIDDIARGTIAALKPLGYEVINLGGHEVITINELVEMVEDLTGKKAVVQHGPPN
                     LADMRTNQADVSKAKELLNWHPQVTLREGIGNLINWYNAEREWAKEVLTP"
     gene            9378..>10204
                     /locus_tag="PLE39_16205"
     CDS             9378..>10204
                     /locus_tag="PLE39_16205"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNE69927.1"
                     /translation="MRSKFKNDPLMSRVLRSSAHLFTSNTISLGLNVLMTALVFRLLG
                     AAGSGLIALVMGYASTVNSLLSFRMSELVVRYGGEHLEKGEEEKASALIKAAGLTEAA
                     VSVLAFLAVLISSVWASRIFTGETNSALLFIAFAVGLLANFNTETSTGILQVTGKIRY
                     QGTINLIQSILSLGIVAAAFFLPNQTWVVLLAYLTGKVVLGLGLFFTAQNQLAQKLGA
                     HRAWHRGPLMPFSESRALIRFAVSSNISATIIKVFRESEPLWVGYFLSTEAVGYYKAA
                     "
BASE COUNT         2356 a         2603 c         2781 g         2464 t
ORIGIN      
        1 tcgaacgctg aaatcttttg tttccattct tccggtacgg gaatggtctt taacccctcg
       61 taatcgaatg tgaccattac cacggtgccg ctggccatca gtttgccggt cttcgcatcc
      121 atcacgcatt gctccaccgt gatggacttg gttccgatcc tggtgatttt cacgcccact
      181 ttaatatcgt cgccgtaatg ggtggtggaa tgataggtga tgtggatatc ggcgatgatg
      241 acacccactt ccataaaaga ctgatccttg ttgaataacc caagtttgat cagatagtaa
      301 atgcgcgcct gctcgaaata agtcaggtat tttgcgttgt tcacatgtcc ctgcggatca
      361 agatctccgt agcgaacttc ggttggatgg aagaatttgt aatcgctcat ggcgggatta
      421 tagttgttag ttaggtagct gagtagttat taaattgccg tccgttgaat tggttttcaa
      481 ctgacggcaa cagcgcatct accagaccga ctcaattgca aaaatgcatg agcccggcct
      541 tgttgtgaat cacaaaccgc ccatctgcat ccgtgctgac gagtccctgc tctttgaaga
      601 ataccatcgc ctgattgacc cgtttgcgcg atgcccccac caggtcagcc aggtcgcctt
      661 gtgtcagcac gatgtgaacc tgcacaccgt cggcaatttc gtgtccatac cgttcggcaa
      721 aagccaacag ttgacgtgcc acccgcccgt tcacatccag cgttgccagc gattgaatga
      781 cctgatctga aagccgtaca cgtgcggtta agattttcac caaattgcgc gccacaggcg
      841 ggaagttatc gagaatatgg tagaaggtgg tcttgtccat ccacagcatg agcgaatttt
      901 caagcgtgat cgcgctcgcc gaacggccga cactgtctat caaactcatt tcaccgagaa
      961 tgtcgccgct tccgagcact gaaataatca catcccgctc cccctgctca atgtggatct
     1021 tcaccgtgcc atgcaaaata atgaacaccg cttcgccggg ctgctcgatc gtcaacacat
     1081 tggtccctgc agtgaaaatg cggcggtgcg catgttttgc cacccaatcc aattgtgcag
     1141 aactaagccc ttcgaacaac ttaatgtctg aaaggatgtt gcgggtatcg tcacgtttga
     1201 tttcggtcat tcttaacata ttttacatcc attcagcgag gggaaacatt accccgtctc
     1261 gcttgtgttg ggccagtcaa aaccattcca gcctgttcgg gtttcaaacg aatctcaagg
     1321 ccttcatacg ctgcgcacga atcagggaat aactttttcg cggcggcttc ctgtgcggcg
     1381 acccaatcgt cagagtaggc ggggtcatga tgaaacagga tcagttcacc cgtctcggca
     1441 gaagcggcca cttccgcggc catcaaggcg gtcgaatgtc cgtatccctg cgtggatggg
     1501 aatcccgcca acattccgcg gtaatgttcc tcgctgtatt gcgcatcgtg gatcaacaca
     1561 tctgcatcac gcgcaaaagc ggccagcttg cgatccatcc ccacatagcc ttcggtgtcg
     1621 gtggcataca ccaccgattt accgcgccac gaaatccgat agacatacac accgccggga
     1681 tgcgcgtacg atcgatgaat gcggatgaca accgcctccg gacttaatcc tgaagctgat
     1741 ccaacaaccc gaacattttc ttcatcccaa acaatgacct gcgactcgcg caccgactcg
     1801 atctgtttgg cggcggccat atcgcgcaag ctgagcggga acgtctctgc cgattgattg
     1861 tgttcaagga catgcttcac ggtttcaggt gtgccgcccg ggccgaagat gtgcagccgc
     1921 gtgttcggga tataagcggg gatgaaaaat ggaaatccct gggtgtggtc gtgatgcagg
     1981 tggctgaata acaacagcag ttccagcgga cgtttttgcc ctgccaattc acgccccagc
     2041 ggaatgatgc ccgtgcccgc atcgagaatg atcgtgcgcc cgcccgcgct gacctccaca
     2101 caggaagtgt tgccgccata tcggatcgta tccgcgccgg gggtggggta acttccgcgc
     2161 acaccccaga atttgatctt cagttcatcg ttcatggcaa cctccgttct gattccatat
     2221 tttgtacttg caagtttatt atcgtcttta tggctttttt ctcaaggaaa gttttcgcat
     2281 gcaggctggg aaatatgtca cacgctgacc atattgttta caaaccatta acccatgcaa
     2341 agcggggatg caagccgtgg cggtataatt ccatccatgc tacctgactt gcaattgaaa
     2401 gatcacctcc gactacgcaa gccgcaccca tgcggttcct atgaatggac agtggtgcgc
     2461 cttggggcgg atatcggttt ggagtgcaga ggctgtcaac accgcgtcat gctgacccgc
     2521 cgtgaattgg cgaaacgatt gaagaccaat ttgacgaagc atgaagagaa aaaagaggaa
     2581 gggaagtagc aggtgcccga tcccgcaggt ttggatgaat tgaccattga atccaaacct
     2641 gcgggatgtt tgcttcataa aaggatctca ggagaaaata aaaacatggc agaaaccgaa
     2701 gcacgatcag ttccaaaggt ggggtcactt cgcattggtt tgttcacaga tacgtatgcg
     2761 ccgcaagtta atggggtttc catttccctg caattgatct ctgatgggct gcgccgccgc
     2821 ggccatcagg tgacgatttt tgcgccgcgt ttcccggggt atgaagataa tgaaaccaac
     2881 gtcatgcgtc tgccctcgct gaaatatttg aacaacccgc caatctatgt ggcggtgctt
     2941 ggaacccccc gttccacctg gtcgctgaca cgcaagcatt tcgatgtcct gcacgcgcac
     3001 agcccggcca gtgtgggcct gctggcctac cttacggcct ccacaaaacg gcttccgctg
     3061 atctacacct accatacatc cattaccgat tacacccatt acgtaaaatt cattggcggg
     3121 tcaagcatca ttaaacacgc ggcttcctgg ttcagcacca cctccaccaa cctgggcgat
     3181 cagatcgttg tgccttcacc aaagttccac cgcctgctgt tggagcagaa ggtgaaacgg
     3241 ccgattcata tcattccgaa tggcattaat ctggatggtt tcaaatctgc caaaaacccc
     3301 ggcagtttta gaaaccgatt gggtatgcaa cccgatgcgc cgattttgct ttcggttggg
     3361 cgcattgacc cggaaaagcg tctggatttc ctcattgacg ccttcgatcg cattgcggac
     3421 catgttccca atgcccgcct tgtttttgcc ggggatggaa gcgcgcgcaa aaaattggaa
     3481 gagcatgccg cttcggtcag gtcgaaagac cgcattcatt tccttggcat ggtcaaccgt
     3541 gccgatctgc cggacctgct gcacgatgcc gcggttttcc tttccgcttc caccacggag
     3601 gtgcatccca tttcggtgat cgaggccatt gcggccgggc tgcctgtgct ggctgtgcag
     3661 gatgaagcgt ttgaaggcat ggtgatcgat ggagagaatg gtcaccagac cttgctggat
     3721 gtgaatgtgt acagcgatac gttgttgagc ctgctgaaag accccgaaag attatcccgc
     3781 tatggaaaac gttctgcaga actcagcgag aaatattcca ttgaggggca ggtgcgtgct
     3841 ttggaaaacc tttatattga gtctattttg caaaactggc gcggcagcct ggtttcgcgt
     3901 atttcggcaa ggttgaagtt ataaaaagtt ttacccaaaa gtgtcacgaa gagatgctgg
     3961 aaatggcagg gtttatcctt cgtgacactt ttgtcccctg gtgggttatt ttttctcgcg
     4021 tggattttca gggagtataa ttctcccgcg atgcccattc ttgattctca taccattgaa
     4081 tttttcagcc gaagcccgga acaaacacgc cgcattggaa tgcggacagg ggcgggtttg
     4141 aaggctggtg atgtgatctg tttacaaggt aacctcggcg caggaaagac cacgttcgtg
     4201 cagggtcttg cgcagggatg ggggtcactc gattcggttt ccagcccgac atttattctc
     4261 gtaaaccaat atcgccgcgc aaacggcgac cgcctctttc atctggatgc atatcgcatt
     4321 gagtcgatgc cagaaggcga ggaactcgat ctcgattcca tgttggcgga tggcgccctg
     4381 gtcattgaat ggcccgaacg tttgggcgac ctgatcccca aagaacgcat gtggatcaac
     4441 ctcgaacaca tggcggagga gcaccgtgaa atgcgtttca atgcgaacgg caaacgctac
     4501 gatgacctgt tggatgaaat ccgtcaatcc atttttggag gggcgtaatg ctgattgctg
     4561 ttgatacttc cacggcccag gttgggctgg ctttatatga tggaaaccag gtgctcggtg
     4621 aattgacatg gacgacccgt cagcatcaca ccactgaatt ggccccggct cttactggac
     4681 ttttgagccg ctccggtgtt tcgatggata tggtcagcgc tttgggggtg gccatcggcc
     4741 cgggctcctt caccagcctg cgggtggggc ttgctctggt gaagggactt gctctggcac
     4801 ggcgcctgcc tgtgatcggc atctccacgc tggatgtgat tgcggcggca cagcctgcga
     4861 gcaagcatcc attgctggct gtgattcaag ccgggcggaa gcggatcgct ttcagcgagt
     4921 ataaaagctt gaagaacgag tggcaggttc aaggtccagt gagaagcggg acggtggatg
     4981 agttggctga ttcgatcgag agtcccactg ttgtggcggg tgaattttcc cccgatgacc
     5041 gcagccgtct ttcgaagaaa cgcgccaaaa tcctgctggc ttcacctgca tattgtgtgc
     5101 ggcgaccatc gcttcttgcc gagttggcct gggaacgctg gcaaaaaaat gaagtggatg
     5161 atgccgcttc gcttgcgccg atctacctgc atgtggaagg gacaccgatt ccgtcgtagt
     5221 ccatcgggaa attttgagca tgaacgtcct ttctcccgac gatgggtcgg ggctgatgaa
     5281 gatacgcaag atgactgtgg atgacctgga gcaggtcatt gccattgatc aggtttcgtt
     5341 tggtttgccg tggcccccgc gttcgttttc ttttgaattg actgataacc cgccctcccg
     5401 ttgttgggtg gtggagatga atggtcgtgt ggttgcaatg ctggttgcat ggctgatcgt
     5461 ggatgagatc cacattgcga cctttgcaac ccaccctgat catcgcaggc agggaattgg
     5521 gggaagattg ctgtatcaca cgctgagaac ggcaagagag gaaggcgcga tcacctcttt
     5581 tttggaggtg cgggctggga atgaatcggc tctggctttg taccgcaagt ttgggtacgt
     5641 ggagagcggc ctgcgcaagg ggtattactc ggataatcat gaagatgcgg tgttgatgac
     5701 cctcggcccc gccgattatg aaagattact gctccatgga gaagcggatg agcgctgaag
     5761 agacccttgc ccaactggcc gtgaaaatat ccacttgcac gaattgtgcg ctgcatgaat
     5821 cacgcaaaaa agccgtgacg ggcgatggcc cggcggatgc ggagatcatg ttcatcggcg
     5881 aaggaccagg ctttcatgag aacgagcagg gacatccctt tgtgggtgcc tctggaaagt
     5941 tcttagacca gttgctggca caggcagggg tgacccgtgc ggaggttttc attgccaatg
     6001 tggtcaagtg ccgcccgccc ggcaaccgcg acccattgcc cgaggaattg gccgcgtgtg
     6061 acgaatatct tgaagcgcaa attcaaacca tcaatccgag tatcattgtg acgctgggcc
     6121 gcttttcgat gaacaagttc atccccggcg cgaagatcag tgccatccac gggcagatgc
     6181 gcaaagtggg cgaccgcttt gtcattccca tgttccaccc tgcggcggct ttgcatcagg
     6241 cagcgctcaa accgtccatt ctggcagatt ttgccaacct gcccgatcaa ttgaacgaag
     6301 cccgcgctgc attgggccga aaggttgttg cgcaaaaaaa ggaagcggct gcgccaaaaa
     6361 atgaaaagcc aaggcagttg agtctgtttt agtcaacgga ttttgaagcg gataggcgga
     6421 ctggagtgac cgatgcctga agaaattaat gcgacacaaa aaccttggta tgaacggttt
     6481 cttgaatatg tgattgggtt gttgcttttg gcttcgctaa cttatggaat cagccattcg
     6541 tctttcagac gtaaaatcga taaggatgaa attgcgcaaa aggttctcga aaattgcaaa
     6601 taccattctt ttatgtatat gcaggaggaa cctttgcaag caaatgccga attgatgacc
     6661 tgtagtgaag tgcaaataaa aactgaaatg aatgaatgtg ataatcgcaa tccggatatg
     6721 aaagtatggt ttgtagctac cgacggttta tggtttttgt acggtcctgt tgttggaaac
     6781 ggggatcttc cacccccaat acttattaat tcttgccatg ctctggttga tgcgagaaat
     6841 ggaatgatcc ttgagacagg gtattcaaaa tttaaaaaat agcttgtgat atgcaggttt
     6901 cggcgggttg caaacccgcc ctacgaaagg aaaaaatgtc taccaaagaa acactcatca
     6961 agaaattgaa ggaaaagaat gcgaagatcg ccatcctcgg cctgggttat gtggggctgc
     7021 cgctggctgt ggtatttggc gaggcgggct ttaacgtcac cggggtggac cctgacacgc
     7081 gcaagatcga agcgttgaac aagggtgtgt cttacatccc tgatgtgaaa acagaagcgg
     7141 tggcgaaact cgtgaaggat ggaaagttca ctgccacggc ggatttctcg gtgttgaagg
     7201 aaatggatgc cgtcagcatt tgtgtgccga ctccgctgcg ccagaccggt gaccccgata
     7261 tgtcgttcat catctccgcg accgagcaac tggcgaagta catgcacaag ggcatggtcg
     7321 ttgttttgga atcgaccacg tatccgggca cgacccgtga actgcttctg ccaaaactgg
     7381 gagttgctca cgacctcaag atcggcgagg actggttcct ggccttttcg cccgagcgcg
     7441 tggacccggg ccgcgaagat ttcactacca tcaatactcc caaggtgatg ggcggcatca
     7501 ccgaagcctg tggtgaagtg gcaaccgcct ggtatgaagg tgcgatcaag atcgtgcatc
     7561 atgtttccac ggcagaggct gcggaaatgt cgaagctgct cgagaatacc ttccgcatga
     7621 tcaacattgg cctggtcaat gaactcgcca tcatgtgcga acgccttggt gtggatgtgt
     7681 gggaggtgat cgatgccgcc gccaccaaac cgttcggatt tatgaaattc accccggggc
     7741 ccggccttgg gggtcactgc attccgatcg acccgctgta tctttcgtgg aagatgaaat
     7801 cgttcaacta caatgcacgc ttcattgaac tggcctctga gatcaacacc aatatgccgc
     7861 gctatgtcgt ggcacgcatc ctcgatgcga tgaatgaccg cggaatgatc ctgaagggct
     7921 cgaaagtgct tgtgctgggc gtggcttaca aacccgatat tgacgatgtc cgtgaatcgc
     7981 ctgcgttgga tgtgatcggc cttttgcaaa agaaaggtgc ggtcgtggaa tatcacgacc
     8041 cgtacattcc gcacatccac cacgaatatg acggctggca catggacagc atcagtgatg
     8101 tgatgaagtc cgtccaatct gccgatgcgg ttgtggtcgt cacgaatcac aaatcctacg
     8161 attacaaggc cattgttgat gctgcgaagt ttgtgttcga tacccgcaac gccaccagca
     8221 aggttgcgaa gaacagcgaa aacgttgtaa gactttagag gaaaccatgg gacattatct
     8281 ggttacaggt gcggcaggct tcatcggtgc gcggacttct gaacttctga tcgggcaggg
     8341 gcataccgtg gtcggcattg acaacatgaa cgacgcgtac gatccgcgca tgaaggaata
     8401 tcgcttgaag aaactgcagg cgatgaaagg tttcgaattc gtccgtgatg acatttcaga
     8461 caaggggatt ctgaaacggc aggcggcgct tgtcagccgt ccgttcgatg gtgtgatcaa
     8521 tcttgcggcg cgggcgggcg tgcgcttcag cgtggataat ccctgggtct ttttggagtc
     8581 taatgtcttg ggcaccttga acatgctgga gatctgccga cagtatggat gcaagaaatt
     8641 cattctcgct tccacttcca gtatttatgg cgaaaatccg ccgtacccga cacctgaaac
     8701 cgcctcaagc agtgaaccca tgcagccgta tgccgccagc aaaaaaggcg cggaggcatt
     8761 ggcacattcc tatcaccacc tgcatgacat tgacgtgacc atcgtccgct atttcacggt
     8821 ctatggcccc gctggccgcc ccgatctggc catgttccgc ttcgtgcagt ggattttgga
     8881 aggccagccc atccgcatca atggtgatgg aaatcaatca cgcggattca cctacattga
     8941 cgacatcgcc cgcggaacca tcgccgcctt gaagccgctt ggttacgaag tcatcaacct
     9001 tggcgggcat gaggtcatca ccatcaatga gcttgtggaa atggtggaag acctgacggg
     9061 caaaaaagcc gtcgttcagc acggtccgcc caatctggcc gacatgcgca ccaatcaagc
     9121 cgatgtgagc aaggcaaagg aattgctcaa ctggcatccg caggtcactc tgcgcgaagg
     9181 catcggcaac ctgatcaact ggtataacgc cgaacgtgaa tgggccaaag aagtcctgac
     9241 gccgtaaaga atagaatcga ccgaatccaa aacaagtcca cggagaaagc agagaatgtc
     9301 tctgcgccct ccgtggactt tcttgttgtc gcctgcaggt gttctctgta gcgggaaatc
     9361 ttgtgtacaa ttcatccatg cgatcgaaat tcaaaaatga cccgctgatg agccgtgtgc
     9421 tccgttccag cgcacatctt tttaccagca acaccatcag ccttggttta aatgtgctga
     9481 tgacggcgct cgtctttcgt ttgctgggcg cggcgggttc gggcttgatc gcgcttgtga
     9541 tgggttatgc gtccacggtc aacagcctgc tttcgttccg catgagtgaa ttggtcgtgc
     9601 gttatggcgg cgagcatctc gaaaaggggg aagaggaaaa agcatccgcg ctcatcaagg
     9661 ccgcagggct gaccgaggcc gccgtttcgg tgctggcttt tttggcggtg ttgatttctt
     9721 ccgtgtgggc ttcgcgcatt tttacaggcg aaacaaattc agcccttttg tttattgcct
     9781 ttgccgttgg gctgctggca aatttcaaca cggaaacttc cacaggtatc ttgcaggtga
     9841 cggggaaaat tcgatatcaa ggcacgatca atttaattca aagtattctc tcgctgggca
     9901 ttgtggccgc ggctttcttt ttgccgaatc aaacctgggt cgttttgctg gcttatctca
     9961 cgggcaaggt cgttttgggg ttggggttgt tctttaccgc ccaaaatcaa ctggcgcaaa
    10021 aactcggcgc gcacagagcg tggcaccgtg gcccgctcat gccgttttcc gaatcgcgcg
    10081 ctttgatccg tttcgcggtc agttccaaca tcagcgcgac catcatcaaa gtgtttcgcg
    10141 agagtgagcc attgtgggtg ggctattttc tttcgacgga agcggttggg tattacaagg
    10201 cggc
//
LOCUS       DAOQJC010000137        10134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_3467_length_10134_cov_10.398849, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000137 DAOQJC010000000
VERSION     DAOQJC010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10134
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3467_length_10134_cov_10.398849"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            448..4710
                     /locus_tag="PLE39_16210"
     CDS             448..4710
                     /locus_tag="PLE39_16210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE69928.1"
                     /translation="MNRTKPAPESYQLPRLMRSRPFGVGEDGENVGRTKGTIISSTLK
                     YMLKQVELRTKDVQAVEEAKHELVRRINEAIADPRHHVTLEYLLNEGNVYSAEFDLYF
                     SEITRQVSGQSFDEFHFMRAANSVPEAIAVLVRPLSLRQVYNLLPRFAAKFADTDFRV
                     ISVKGNSAVIQWRCARELQTLPPETHDVFIHESCLFVQGSLSHVPTVVSGDSPARVIE
                     RKCQLWGDDCCEYEFVWEARNLLERLFGKKNTQAVPAIPAASALNERVPELQRKTMDF
                     PPLPERMTFHPYGLDENGKRIVNTDAATLRSVMEYLKECIARNVEAALPVDVPAVLRE
                     RQVAEAQAQALDLLVTKLNAAMPDARFHVTMEDMTRDGNAFSAEFSSFFAIYCNELSG
                     DPKFDFNRGVKMGYLAPWLIRPLTLRRTFIIMPNLVARFFKADIRVLNVTPTSAVLQW
                     RVGDHLLQLPKSMHGRYVYDVCQVFQGVFSEIPRVHSNLPPAQIRETKCQAYGDDCCE
                     WEFVWEAPQTRNLWNIFKARQGGKASEVLFSAVPASPSPELPRLPRKLISHPYGMDAD
                     GRPIKDLNGLFLRVNIDFMLAAVARRAVENLPNGMTAETAVTQAQQAAMENLLEQVNA
                     CLPKSQHLTQEGLLGLGYASFDLGTVIRETCAEIAQVPNFFFHQGFAIVQSVVYLLRA
                     LSIRQVFTGVPRFASKFGNIDLRVVNDSATSAILRWYPDPTLERSSPYTHQHVVNMTC
                     QTIQGSMAYVPPLVAGLPPAQVREIKCRLHGDEYCEWEFTWQLPRPSRYRSVWLGGLI
                     TLLLLANFYFQGTGWQWVNWFALAFFPWLSGWLLNRWSQRGYQLDRREKLLLEQRDAS
                     EQQYDALQKSNAELQLANLTLQEKIAEVTALTETLEQRVVDRTREVEEARKQAEDANR
                     AKSTFLASMSHEIRTPMNGIIGMAGLLFDTRLTPEQREFAETIRSSSDALLTIINDIL
                     DFSKIEAGKMDLESHPFDLRECVESAVDLVALKASEKGLEVGLLIEPNVPEAVLGDVT
                     RLRQILVNMLSNAVKFTEKGEILITVQNETPLLHFTIKDTGIGIPAERLSSLFQSFTQ
                     VDASTTRKYGGTGLGLAISKRLSEMMGGTMWVDSIAGAGSTFHFTVQTPPTTATTTPK
                     LIVPTQLRGKRVMIVDDNETNRRILTLQSENWGMRPVAYATPLEALTAIRSGERFDVA
                     VLDMHMPDMDGISLASEIRKYEAARHLTTPLLMLTSLSSREEMNADLFSAFLTKPVKQ
                     SVLYNALIEALAIEVAAPIQTASQEQQFDHTLAERIPIRILLAEDNAVNQKLALRMLD
                     RMGYRVDVAANGLEVLEALGRQFYDLIFMDVQMPEMDGLEATRAIRVMPSLTRQPRVV
                     AMTANAMQGDREMCLEAGMDDYISKPIQVKELQRAIEEMAIK"
     gene            complement(4810..5958)
                     /locus_tag="PLE39_16215"
     CDS             complement(4810..5958)
                     /locus_tag="PLE39_16215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE69929.1"
                     /translation="MKIALLSEKYTPDLGGLAISAGRMGHLLDSAGHKVRVFAPSPNL
                     PASEKQTLPINGISVTRFGTRKRVDDTLVDWFELIEAEHKREPFDLIHAYFLPMAGFV
                     GAYAGKFLGIPSVVSIRGNDIERAAFDPSKFSHVMYALQNASAVTTNASVLAKKAKAL
                     IEREIHLIPNGIDTNIFKPLERNEMLAEAIGLNVGKKKEERNFVIGFVGELREKKGLA
                     TLLSGYAQTAKEMPVSLLIVGEIREGEDKRVLEEFQAINPQLQITITGHIPHRDLPAY
                     YSLMDIFIHPSLRDGMPNAVLEAMACEKAVIATPVGGVLDVIEDEKNGRMVPVNDAET
                     LAQEIKALLNDEQLRKQLGENARKKIQAEFTPQKELEANLRIYKSLQS"
     gene            5970..6260
                     /locus_tag="PLE39_16220"
     CDS             5970..6260
                     /locus_tag="PLE39_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69930.1"
                     /translation="MAEKCEMIIGYLVPHRCENPALGACVKCGRGFCDEHTNATREGR
                     VCLACQQGLEMPVALPIAAALFTPDDLSTFDRTRRFNDDVDDENGELFSDLS"
     gene            complement(6266..7330)
                     /locus_tag="PLE39_16225"
     CDS             complement(6266..7330)
                     /locus_tag="PLE39_16225"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE69931.1"
                     /translation="MYNLDTLANEKDDFHKAVLNARAFKPLYVKIKINYGCNLKCAMC
                     KHWRETREAPIPMDRFKEVISELAELGTKKIHFSGGEPMLRPQLPDLVAHATGLGMRV
                     TLTTNGTLINKEKAKALVEAGLRGVNVSIDSPIRKVHEKVRGVEGSFKLTTKAVSLFN
                     KYSHKGKLTVRINTVVSRENYFSLATLPELAHELGADGINLIPVDDHCGEHLSMRKKD
                     IALFNEEIAPYIEKRASELGVNIADEDAFPFGRDESEVKLGRAGRYAFGYYSKFPCYA
                     PWTHSLIDFNGLVYVCCMTREQIPPLGDIRKQSFKEIWEGLAYQNVRQKMHPPSLKPC
                     QRCDDFILENKKIWETVGPY"
     gene            complement(7407..8273)
                     /locus_tag="PLE39_16230"
     CDS             complement(7407..8273)
                     /locus_tag="PLE39_16230"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE69932.1"
                     /translation="MFRKTSQVNEYRVLINDQPWNIFEAGSGTPMLFLHNGGGTLWNW
                     AHQLQYFTDTYRVIAPDLPGFGRSHRPAQPLTLDAYVHGLERLLEILDCPQPILVGNC
                     IGASIALEFALRYPEKVSALTLFNICGGPPMLNPFLRVWSTAQPITAFGVTAHRVMIE
                     AAAHPALRKFGTSLLYADHEPTLHPQLESFLQAQQSDPDLRASLYWLAKGLDSFSIFS
                     QPRQKPVHFPPVLLGWGAKNRTLHPRWADQIADWLKPQEFWLVENAGHMPMYEQPEQV
                     NARLEQFLRVHL"
     gene            complement(8249..8701)
                     /locus_tag="PLE39_16235"
     CDS             complement(8249..8701)
                     /locus_tag="PLE39_16235"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE69933.1"
                     /translation="MIKLIRTNSENTDFIELVRHLDADLAERDGSEHAFYAQFNKIAK
                     IKHVVVAYEDGTPIGCGAIKEYETGTMEVKRMYTTPASRGKGIASRVLSELECWAAEM
                     SCQKCVLETGIKQPEAIALYKKNGYQIIPNYGQYIGVDNSLCFEKLLK"
     gene            complement(8786..>10134)
                     /locus_tag="PLE39_16240"
     CDS             complement(8786..>10134)
                     /locus_tag="PLE39_16240"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNE69934.1"
                     /translation="CNSKIPTHGVNVMEKKQLSRLILTAIMVFTLACNAVTQMIPSTG
                     QETPTNIPPSPVDTATPAPTAIPPTPTLAVTTMTSEKDGATLVLVPAGEFTMGRGDAS
                     DSSDYAPAHIVYLDAYWIDQTEVTNGMYAQCVQAGGCKRPTDTSSNNNPSYYGNPEFD
                     DYPVIYVEWDMAKAYCKWAGRRLPTEAEWEKAARGTDGRSFPWGEDTISGFENFAMIV
                     GDTTKVASYEKGKSPYGAYDMAGNVHEWVNDWYDYEYYKNSPSSNPQGPDVGRPPARV
                     LRGGSFVEDGFFSLTTTRIWNLDTLIGVGFRCASSDNIATTDSPNQPPAQQVTCADGV
                     VIDYTETPKGVVLEICAFNESYEIPGLAKGAFAVGPNNKFFVYCTNGGDVFAVRIGDT
                     QMQLIGDVKDFYMIRRGEVPEFAFSFEGDHPYKVTVREIKSNETQVFPIPRFVSAP"
BASE COUNT         2232 a         2429 c         2808 g         2665 t
ORIGIN      
        1 tttacacagg tcaatgcctt gaccaggctc gggtatgtgc tgatgggaat attgctgatg
       61 gggaagtttt caagcgcccg tgctgtgtgg acaccataat atacgtcact gggaacttca
      121 cggtcaccta aaaggtcatg ttcaatgcga tggttcactt ttgctccaga gttttgattt
      181 gcgtatccct gtgttgcgag tgactggaac cctgtttcag cttagagtcg gcttgtaagg
      241 cgctggcagg tcacgggtcg gcagggatgc ggatttttat tccaaaattt tggaatgctt
      301 tgacaaaata atgataccat tgtgaaaatc aaattttaaa ggaatttgta gtgtaatctg
      361 ccgcaaaacg gatctggatt tatctgaggt ttgaagttta tgagtatatc ggaacttaat
      421 tcccctttgc agacaaagag cggatctgtg aacaggacga aacctgctcc agagtcatat
      481 caattgccgc ggttgatgag atcgcggccg tttggtgtgg gtgaggatgg agaaaatgtc
      541 gggcgtacca aggggacgat catcagttcc actttgaaat acatgctgaa acaggtggag
      601 ttgcggacga aggatgttca ggcagtggaa gaggcaaaac atgagttggt gcggcggatt
      661 aatgaagcga tcgcagaccc acgccatcat gtgactttgg aatatctgct caacgaaggt
      721 aatgtatatt ccgccgaatt tgatttgtat ttttccgaaa taacacgcca ggtatcgggg
      781 caatcatttg atgaatttca ttttatgcgg gctgccaaca gcgtgcctga agcgattgct
      841 gtgttggtgc gtccgctttc gttgcggcag gtctataacc tgttgccgcg ttttgcggct
      901 aaatttgccg atacagattt tcgagtcatt tcggtgaagg ggaattcggc cgtaattcag
      961 tggcgttgcg ccagggaatt gcagacgttg ccgcctgaaa cacatgatgt gtttattcat
     1021 gaaagctgcc tgttcgtgca gggatcgctt tcgcatgtgc ccactgttgt gagcggggat
     1081 tcgcctgcac gggttattga acggaaatgc cagttgtggg gcgatgattg ttgtgaatat
     1141 gaattcgtat gggaggcaag aaatcttttg gagcgtttat tcggcaagaa aaatacccag
     1201 gctgttccag ccatacctgc tgcttcggct ttgaacgagc gcgttcccga actccaacgg
     1261 aagaccatgg attttccgcc attgcctgaa cggatgacat tccatcctta tggattggat
     1321 gagaatggca agcgtatcgt caacactgat gccgccactt tgcgcagtgt catggaatat
     1381 ttgaaagagt gtattgcgcg gaatgttgaa gccgctctgc cagtggacgt gcctgcagtt
     1441 ctgcgcgaaa gacaggtcgc agaagcacag gcgcaagccc tggatttgct ggtgaccaaa
     1501 ctcaatgcag ccatgcctga tgcgcgcttc catgtgacga tggaagatat gacgcgtgac
     1561 ggcaatgcat tctctgccga gttttcatcc ttctttgcca tttattgcaa tgaactttct
     1621 ggtgacccga aatttgattt caatcgtggt gtgaagatgg gctatcttgc gccgtggctt
     1681 atccgcccgt tgactttgcg gcgcactttc atcatcatgc ccaatttggt ggctcgtttt
     1741 ttcaaggcgg atattcgtgt gctcaatgtc accccaacca gtgcggtgct tcagtggcgg
     1801 gtgggcgatc atctgttgca gttgcccaag tccatgcatg gacgatacgt atatgacgtt
     1861 tgccaggtgt ttcagggagt gtttagtgaa attccgcgag tccattccaa cttgccgcct
     1921 gcgcagattc gtgaaacgaa atgtcaggca tatggcgatg attgttgtga atgggaattt
     1981 gtttgggagg ccccccaaac gcgcaatttg tggaatattt tcaaagccag gcagggcggg
     2041 aaggccagtg aggtgctttt cagcgctgtc cctgcttcgc ccagccccga gcttccacgg
     2101 ctgcctcgaa aattgatttc tcatccctat ggcatggatg cggatgggcg gccgatcaag
     2161 gatctgaacg gtctcttcct gcgtgtaaat attgatttca tgctggcggc tgtggcgcgt
     2221 cgtgcagttg agaatctgcc caatggcatg accgcagaaa ctgcagtgac gcaggcgcaa
     2281 caagccgcaa tggaaaattt gctggaacag gtcaatgcct gcctgcccaa atctcaacac
     2341 cttacgcaag aagggttgtt ggggttgggc tatgcatcct ttgacctggg tacggtcatc
     2401 cgtgagacat gtgccgagat cgcccaggtg ccgaactttt tcttccatca agggttcgcc
     2461 atcgtgcaaa gcgtggtgta tctcctgcgc gcgttgagca tcaggcaggt ctttaccggg
     2521 gttccgcggt ttgcctcaaa atttggaaac attgatttgc gtgtggtgaa tgacagtgcc
     2581 acttcggcga tcctgcgctg gtatcctgat ccaactttgg agcgttcatc gccgtacacc
     2641 caccaacatg tggttaatat gacctgccag acgattcaag gcagcatggc gtatgtgccg
     2701 cccttggtgg ctggcttacc gccagcgcaa gtgcgtgaga tcaaatgtcg tttgcatggt
     2761 gatgaatatt gtgaatggga attcacatgg cagcttccgc ggccatcaag gtatcgcagt
     2821 gtctggctgg gtggtctgat caccttgctt ttactggcta atttttattt tcaaggcacg
     2881 ggctggcaat gggtgaactg gtttgcgctg gcatttttcc cctggttgag cggctggctg
     2941 ctcaaccgct ggtcgcagcg cggataccaa ctcgatcggc gcgaaaaact tttgcttgaa
     3001 cagcgcgatg cctcagaaca gcaatatgat gccctgcaaa aatcgaacgc tgaattgcaa
     3061 ttggcaaacc tgactctgca ggaaaagatc gccgaagtga cggcactgac cgaaacattg
     3121 gaacagcgtg tggttgaccg tacgcgtgag gttgaagaag cccgcaagca ggccgaagat
     3181 gccaaccgcg ccaagtccac cttcctggcc agcatgagcc acgagattcg cacaccgatg
     3241 aacggcatca tcggcatggc ggggctgctt ttcgatacac gcctcacccc tgaacagcgt
     3301 gaatttgcag agaccattcg cagcagcagc gatgccctgc tcaccatcat caatgacatc
     3361 cttgatttct ccaagatcga agctggaaaa atggatctcg aatcgcatcc cttcgatttg
     3421 cgtgaatgtg ttgaaagcgc agtggatctg gtggcattga aagccagtga aaagggactc
     3481 gaggtgggcc tgctcattga gccaaacgta cccgaagccg tgttgggtga tgtcacccgt
     3541 ttgcgccaga ttcttgtcaa catgttgagc aatgcggtca agttcactga aaaaggcgag
     3601 atcctcatca ccgtacaaaa cgaaactccg cttctgcact tcaccatcaa agacacaggc
     3661 attggcatcc ctgctgagcg cctatcctcg ctctttcaat cttttaccca ggtggatgcc
     3721 tccaccacac gcaaatatgg cggcaccgga cttgggcttg ccatcagcaa acgtctttct
     3781 gaaatgatgg gtggaacgat gtgggtggat agcattgccg gggctggttc caccttccac
     3841 tttacggttc aaaccccgcc gacaactgcc acaacaaccc cgaaattgat cgtcccgaca
     3901 cagctgcgcg gcaaacgcgt catgatcgtg gatgataacg agaccaatcg ccgcattctc
     3961 accttgcaat cagagaactg ggggatgcga cccgtggcat acgcaacccc gctcgaagca
     4021 ctgaccgcca tccgcagtgg cgaacgcttt gatgtggcgg tccttgacat gcacatgccc
     4081 gatatggatg ggatttccct tgcttcggag atccgtaaat acgaagccgc ccgccatttg
     4141 acaaccccgc ttctcatgtt aacttccctt tcctcgcggg aagaaatgaa tgcggatcta
     4201 ttttccgctt tccttaccaa gcccgtcaaa caatctgtgt tgtacaacgc attgatcgaa
     4261 gcgcttgcca ttgaagtggc tgctcccatc caaaccgcat cacaggaaca gcaattcgat
     4321 cacaccctgg ctgaacgtat tcccattcgt atcttgctgg ccgaagataa cgccgtcaac
     4381 caaaaactgg ccttgcgtat gttggaccgt atgggatacc gtgttgacgt tgcagctaat
     4441 gggttggaag tgcttgaggc cttgggcagg caattctatg accttatctt catggatgtg
     4501 caaatgcccg aaatggatgg tcttgaggcc acccgcgcca ttcgggtgat gccaagcctt
     4561 acccgtcagc cccgtgtcgt tgccatgacc gccaacgcca tgcaaggtga ccgtgaaatg
     4621 tgcctcgaag caggcatgga cgattacatc agcaaaccga ttcaagtgaa ggaattgcaa
     4681 agagccatcg aagaaatggc cataaaataa aaatatcctc cccaattttc aaaaatgcag
     4741 aattggggag gatgcttttt aagcgattcg ccgtatgctt ttttaccaga tcgctgaccg
     4801 acgttttact tagctctgaa gtgacttata gattctcaga tttgcctcga gttctttttg
     4861 cggtgtgaac tctgcctgga tcttctttcg agcattttcc cccaactgct ttcttagttg
     4921 ctcgtcattc aacaaggctt tgatttcctg cgccaatgtt tctgcgtcgt tgacggggac
     4981 cattctgcca tttttttcat cttcgatcac atccagcacg ccgccaacag gggtggcaat
     5041 gacggccttt tcacaggcca tggcctccag cactgcattg ggcattccgt cgcgtagtga
     5101 tgggtgaatg aagatatcca tcaaggaata ataggcaggc agatctctgt gggggatgtg
     5161 ccctgtaatg gttatctgta attgtgggtt gatggcctga aattcttcaa ggacgcgttt
     5221 gtcttcgccc tctcgaattt caccgacgat caacaacgaa acgggcattt ccttcgcggt
     5281 ttgcgcgtag ccagaaagaa gggttgctaa tcctttcttt tcgcgcagtt ctcccacaaa
     5341 gccaatcaca aagtttcttt cctctttctt ctttcctacg ttcaacccaa tcgcttctgc
     5401 cagcatctcg ttccgctcca gcggtttgaa gatgttggta tcgatcccgt tggggatgag
     5461 gtgaatttcc cgttcaatca atgccttggc tttcttggcc aatacgctgg cattggtcgt
     5521 cacagcgctg gcgttttgca gggcgtacat cacatgtgaa aacttcgacg ggtcaaaggc
     5581 ggcgcgttca atgtcgttgc cgcggatgga gacgacgctt ggaatgccca gaaattttcc
     5641 tgcatacgca cctacaaatc ctgccatcgg aaggaaatag gcgtggatta aatcaaacgg
     5701 ttcgcgctta tgttctgctt cgataagttc gaaccaatcg accaaagtat cgtctacgcg
     5761 tttgcgtgtg ccgaagcggg tgacgctgat tccgtttata gggagagtct gtttttcgga
     5821 ggctggcagg tttgggcttg gggcaaagac gcggacttta tgtccagcag agtcaagcag
     5881 atgccccata cgtcctgctg agattgcgag accgccgagg tctggtgtat acttttctga
     5941 aagcaatgca attttcatga ggtgtctcaa tggcagaaaa atgtgaaatg attatcggtt
     6001 atttggtgcc gcatcgttgt gaaaaccctg cactgggagc atgtgtgaag tgcgggcgcg
     6061 ggttttgtga tgagcataca aatgccaccc gggaaggacg tgtgtgcctt gcctgtcagc
     6121 aggggttgga gatgcctgtt gccttgccga ttgccgccgc gttgttcacg cctgatgatc
     6181 tgagtacctt tgatcgcaca cgtcgtttca atgacgatgt ggacgatgaa aatggagagt
     6241 tgttttctga tctgagttga ttgagttaat acggtccgac cgtttcccag atttttttat
     6301 tttccaaaat aaagtcatcg cagcgctgac agggtttgag ggagggcggg tgcattttct
     6361 ggcgcacgtt ttggtaggcc agcccttccc agatctcctt gaatgattgt ttgcgaatgt
     6421 cgcccagcgg ggggatttgt tcgcgggtca tgcagcacac atacacgaga ccattgaaat
     6481 caatgagact gtgcgtccac ggggcatagc aggggaattt gctgtagtag ccgaatgcat
     6541 acctgccggc gcgtcccaat ttgacctcgg actcgtccct gccaaatgga aatgcatctt
     6601 catctgcaat gttgactccc aattcgctgg cgcgtttttc gatgtaggga gcaatttctt
     6661 cgttgaatag tgcgatgtct ttctttcgca tggagagatg ttcgccgcaa tggtcatcca
     6721 cagggatcag gttgatcccg tcggcgccga gttcgtgggc gagttcgggc agggtggcaa
     6781 ggctgaaata attttcgcgg ctgaccaccg tgttaatgcg gacggtcaat ttccccttgt
     6841 gagaatattt gttgaaaagt gagaccgctt tcgttgtcag cttgaacgag ccttccacac
     6901 cgcgtacttt ttcatgcact ttgcgaatcg gcgaatcgat ggaaacgttc acgccgcgta
     6961 accctgcttc gacgagggct tttgcttttt ccttgttgat gagtgtgccg ttcgtggtga
     7021 gggtgacgcg cattcccagc cctgtggcgt gtgcgacgag gtcgggcagt tgcgggcgca
     7081 gcatgggttc accgccagag aaatggatct tctttgtgcc cagttccgcc aattcgctga
     7141 tgacttcctt gaacctgtcc atggggatgg gcgcttcgcg ggtttcgcgc cagtgcttgc
     7201 acattgcgca tttgaggttg cagccatagt tgatcttgat cttgacataa agcggcttga
     7261 acgcgcgcgc gttcaagacc gctttatgga aatcatcttt ttcatttgca agggtatcga
     7321 gattgtacat ggattctcta cgggtaaaag ggaattgaat ttaatccatt gtaacacttt
     7381 gaaaattttc cacctatcat ctatttttaa agatgaacac ggaggaactg ctccagcctt
     7441 gcgttgacct gttcgggttg ttcgtacatg ggcatgtgac cagcattttc gacaagccaa
     7501 aactcctgcg gtttgagcca gtcggcgatc tgatcggccc agcgcggatg cagggtgcgg
     7561 ttttttgctc cccaccccag caagacgggc ggaaaatgaa ctggtttttg ccgtggttgg
     7621 ctgaaaatgc tgaaactatc caaacccttt gcgagccaat acaacgaggc ccgcagatcg
     7681 gggtcgcttt gttgggcttg taaaaaactt tcgagctgtg gatgaagggt cggttcatgg
     7741 tccgcgtata gcagggatgt gccaaacttg cgcagagcgg gatgggcagc cgcttcgatc
     7801 atgacccgat gggctgttac cccaaatgcg gtgatgggtt gcgctgtaga ccagactcgt
     7861 aaaaatggat tgagcatggg cggaccgccg cagatgttga aaagcgtcag ggctgatact
     7921 ttttcgggat accgcagggc aaattcgagg gcgatggatg cgccgatgca atttcccacg
     7981 agaatgggtt gggggcagtc caaaatttcg agcaggcgtt ccaatccatg gacgtaggca
     8041 tccagggtca ggggttgggc aggacgatgc gagcggccaa agcccggcag gtctggtgca
     8101 atgacgcggt aggtatcggt gaaatattgg agttgatgcg cccaattcca gagcgttcca
     8161 ccgccgttgt gcaggaaaag catgggcgtc cctgaaccag cctcgaagat gttccaaggc
     8221 tggtcgttga tcaggacgcg gtattcattt acttgagaag tttttcgaaa cacagactgt
     8281 tatcaacccc gatgtattgc ccatagtttg ggatgatctg gtagccgttc tttttataaa
     8341 gtgcgatcgc ttcaggttgt ttgatgcccg tttctaaaac gcatttttgg caggacattt
     8401 ctgccgccca gcattcaagt tcggataaaa cgcgggatgc gattcctttt ccacggctgg
     8461 caggggtggt gtacatccgc ttgacttcca ttgtgcctgt ttcgtattcc ttgatcgcgc
     8521 cgcagccgat aggcgtgccg tcttcataag cgacgaccac atgtttgatt ttcgcgatct
     8581 tgttgaattg tgcgtaaaaa gcgtgttcgc tgccatcacg ttcggccaga tcggcgtcga
     8641 ggtggcggac gagttcgata aagtctgtgt tttctgagtt ggttcttatt agtttgatca
     8701 tggtaatttg taaaatggat gatgaatgcc cgccgacagt ggtggtgaac aacggtcggc
     8761 gggcgtggag tgcgataagg tatttttatg gtgccgatac aaaccgtggg atcgggaaga
     8821 cttgtgtttc gttgctcttg atctcccgaa cggtgacttt gtatggatgg tcaccttcga
     8881 acgagaaggc aaattcagga acctctcctc tgcggatcat gtaaaagtct ttgacgtcgc
     8941 cgatcaattg catctgtgta tctccaatgc ggacggcgaa tacgtcgccg ccgttggtgc
     9001 aatagacgaa gaacttgtta tttgggccga cagcaaaggc tccctttgcc aacccgggga
     9061 tctcataact ttcgttgaag gcgcaaattt ccagcacgac gcctttgggg gtttcggtgt
     9121 agtcaatgac caccccatcg gcgcaagtga cttgctgggc gggtggttga ttgggcgagt
     9181 ctgttgttgc gatgttgtct gaactggcgc agcggaatcc gaccccgatc agggtgtcga
     9241 gattccaaat gcgggtggtt gtgagcgaga agaatccatc ttcaacgaac gatccgcctc
     9301 tgagcacgcg tgctggcggg cggcctacat caggtccctg cgggtttgag gagggggagt
     9361 ttttataata ttcataatcg taccagtcgt tgacccattc atgtacattg cctgccatgt
     9421 cgtaggcgcc gtaggggctt tttccttttt cgtagctggc tactttggtg gtgtcaccga
     9481 cgatcatggc gaaattttca aagccactga tcgtatcttc gccccaggga aatgacctgc
     9541 catcggtgcc gcgtgcagcc ttttcccatt cggcttcggt ggggagtctg cgccctgccc
     9601 atttgcaata ggccttcgcc atgtcccatt ccacatagat gacagggtag tcatcgaatt
     9661 cggggttgcc atagtaactg gggttgttgt tggagctggt atctgtgggg cgtttgcatc
     9721 cgcctgcctg aacacactgg gcatacatgc cattggtgac ttcggtctgg tcgatccaat
     9781 aggcatcgag gtaaacaatg tgcgcggggg cgtagtcgct gctatctgag gcatcacctc
     9841 tacccatggt aaattccccg gctggcacaa ggaccagagt ggctccatcc ttttcactgg
     9901 tcatggtcgt gacggcgagg gtgggagtgg gtgggatcgc ggttggcgca ggggtggcgg
     9961 tatccaccgg gctgggcggg atgtttgttg gggtttcctg ccccgtagaa ggaatcattt
    10021 gcgtgacggc attacaagca agcgtgaaaa ccattattgc ggtcaagatc agacgggaaa
    10081 gctgtttctt ctccattaca ttgactccat gagttgggat tttggaatta cagg
//
LOCUS       DAOQJC010000138         9875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_3615_length_9875_cov_11.728208, whole genome shotgun sequence.
ACCESSION   DAOQJC010000138 DAOQJC010000000
VERSION     DAOQJC010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9875
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3615_length_9875_cov_11.728208"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1354)
                     /gene="gguB"
                     /locus_tag="PLE39_16245"
     CDS             complement(191..1354)
                     /gene="gguB"
                     /locus_tag="PLE39_16245"
                     /inference="COORDINATES: protein motif:HMM:NF040906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNE69935.1"
                     /translation="MKTLSNLFKNNIREYGMLIALIVIMIFFYFMTDGILMKPINITN
                     LVLQNSYVIIMALGMLLIIVSGWIDLSVGSVAAFIGSIAGVMIVKWEWNWILVMGICL
                     TIGGVIGAWQGYWVAYLKIPAFIVTLAGMLIFRGLTLVMVRGEAIGPFPEGFRNISGG
                     YIPDFFHYEGFHLTTIVVGVLVSILLIFSDIRTRRNQQKYGFEVTPTYFFIVKQILIT
                     AAVMGFCYLMASYKGIPNLLVIMFPLVTIYSFLTSQTVIGRRIYAMGGNEKAARLSGV
                     NTQRLLFLTFVNMGVLAALAGMVVASRLNSSTPNAGEGFELDVIAACFIGGASASGGI
                     GTVIGAVLGAFLVGVLNNGMSILGVGIDWQKVVKGMVLLLAVLFDVYNKNKSS"
     gene            complement(1351..2892)
                     /gene="gguA"
                     /locus_tag="PLE39_16250"
     CDS             complement(1351..2892)
                     /gene="gguA"
                     /locus_tag="PLE39_16250"
                     /inference="COORDINATES: protein motif:HMM:NF040905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNE69936.1"
                     /translation="MSDVILEMRNITKTFPGVKALDNVSFSVKQGEIHALVGENGAGK
                     STLMKVLSGVYPYGSYDGEIFFEGKECRFHDITQSEDVGLVIIHQELALIPYLSIAEN
                     LFLGNERANFGIIDWNESILRTKELLSKVGLHESPDTLITNIGVGKQQLVEIAKALAK
                     KVKLLILDEPTASLNENDSEKLLELLLQFKENGIASILISHKLSEVSKVADSITILRD
                     GATIETLDCHKDTITEDRIIRGMVGRDITHRFPPRESRIGEVVFEIKDWNVYHPQHED
                     RKVSNNVNIKVHRGEVVGISGLMGAGRTELAMSVFGRAYGTRISGKAFKDGIEIDIST
                     IDKAIANGIAYATEDRKAYGLVLIEEIKDNITLSNLNAISDAGVINEPKELTVTREYR
                     DKLNIKCSSILQLALNLSGGNQQKVVLSKWLFANPEILILDEPTRGIDVGAKYEIYTI
                     INQLASEGKAIILISSELPEILGVCDRIYVMREGKIVGEMPAKEASQELVMKHIMFEQ
                     EAIAQ"
     gene            complement(3067..5169)
                     /gene="chvE"
                     /locus_tag="PLE39_16255"
     CDS             complement(3067..5169)
                     /gene="chvE"
                     /locus_tag="PLE39_16255"
                     /inference="COORDINATES: protein motif:HMM:NF040907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNE69937.1"
                     /translation="MSKKLFYNLLAMLMLASLILSACGGGSSAPASDAKLVGISMPTK
                     TSTRWISDGESMVKSFEELGYETDLQFADNDIPNQLAQIENMITKGANVLVIAAIDGS
                     TLSDVLKKARDAGVLVIAYDRLISKTENVDYYATFDNFGVGVIQGTQIIEGLDLANQA
                     GPFNIELFGGSPDDTNAFYFYDGAMSVLQPYIDEGKLVVQSGQMGMDVVGTLRWDGAV
                     AQSRMENLLSANYTDKRVDAVLSPYDGLSRGIIAALRGVGYGTADQPWPVITGQDAEV
                     ASVKAMIAGEQAYTVFKDTRELAKQVSIMVDAALQGKEVPINDTKTYNNGVKVVPSYL
                     LTPYSVDITNYKELLVDSGYIKAEDLPAAEEAPASSTEPKIVGISMPTKTSTRWISDG
                     ESMVKSFEELGYETDLQFADNDIPNQLAQIENMITKGANVLVIAAIDGSTLSDVLKKA
                     RDAGVLVIAYDRLISKTENVDYYATFDNFGVGVIQGTQIIEGLDLANQAGPFNIELFG
                     GSPDDTNAFYFYDGAMSVLQPYIDEGKLVVQSGQMGMDVVGTLRWDGAVAQSRMENLL
                     SANYTDKRVDAVLSPYDGLSRGIIAALRGVGYGTADQPWPVITGQDAEVASVKAMIAG
                     EQAYTVFKDTRELAKQVSIMVDAALQGKEVPINDTKTYDNGVKVVPSYLLTPYSVDIT
                     NYEELLIASGYIKPEDLK"
     gene            complement(5408..6451)
                     /locus_tag="PLE39_16260"
     CDS             complement(5408..6451)
                     /locus_tag="PLE39_16260"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNE69938.1"
                     /translation="MVIADRRKRVTIKDVAQAAGVSTQTVSRVMNKFSYVSEETRQRV
                     ETVVEQLGYHPSTLARSLIQQRSYTLGVVTFGLKYIGPSRTLNGVADKADELGYMLLM
                     KELDNFDTNRIDEVIDSLLARQVDGIIWVAPEIGDNHAWVEERMHNIPAPVLFLAMKP
                     RAGISSVATDNYQGAVLAIQHLLECGRKKIGHISGPLTWWEADERKRGWFETLKAAGL
                     NPLEEHCAEGNWSSASGEQAFTQLLASYPDMDAVFVANDQMALSVLRETCCRGICVPD
                     QLAVIGFDNIAESAYFYPSLTTISQDLQLLGEQAVQSLVEMIQARQNNQVIVAQSRFI
                     QPTLVVRESSKRV"
     gene            complement(6694..7992)
                     /gene="eno"
                     /locus_tag="PLE39_16265"
     CDS             complement(6694..7992)
                     /gene="eno"
                     /locus_tag="PLE39_16265"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNE69939.1"
                     /translation="MDTTIESISALEILDSRGNPTVEVEVVLMDGAMGRAAVPSGAST
                     GEHEALEMRDGDKKRYLGKGVSKAVANVNGVISEALFGWDASDQKAIDNELLALDGTP
                     NKAKLGANAILGVSLAVAKAAANSYGMPLYRYLGGVYAHVLPVPMMNIMNGGAHTSWQ
                     STDMQEFMVMPFGAPSFSEGLRWGAEIYHALKSVLKEKGYGALVGDEGGYAPALKANN
                     EAIELILAAIEKAGFKAGRGEQVAIALDPAASELYDEKTKKYNLRKEGKELTGEQMVE
                     FWAKWVKDYPIVSIEDGLAQDDWDAWKLMIKTVGDKCQIVGDDLLVTNPERVRRAIAE
                     NAANALLVKVNQIGSLTETIEAVDLCQRAGWRAVTSHRSGETEDATIADLAVALNTGQ
                     IKTGAPARSDRVAKYNQLLRIEAELGSEAHYAGWNALSNK"
     gene            8016..8744
                     /locus_tag="PLE39_16270"
     CDS             8016..8744
                     /locus_tag="PLE39_16270"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE69940.1"
                     /translation="MKLRIKYNRILYGVATLIAQQDFDPRDRKKYYLEETFARRFLLS
                     LVRGVFHSVMVMKVEGRENFPLDGPVIIAANHVTNFDVFPMQFSVSRAICYMGKAELF
                     NTPLDSILRILCAFPVYRGEKDEWAMRHAEKVLANGHTLGMFPEGKRSKGKGLGVAKT
                     GTARLAIKMNCPIVPMNVIGTDTFFKRFPYRSNVTVRLLPPILPNPNDTPLSLTDRLM
                     FTIAEGLPESMRGVYAELPKGFGA"
     gene            8789..9277
                     /locus_tag="PLE39_16275"
     CDS             8789..9277
                     /locus_tag="PLE39_16275"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE69941.1"
                     /translation="MTQESSGNSTPAPKKLIPFLCYAKENQVIVREFSQRLKSEGWID
                     PWFDEEDILPGQVWQDSVVHAVRQSHAVIVFLSKEAVASEGFFQKELKLALDTAAEKP
                     EGTIFIIPIRIDHCEVPEMLAKYQYVDYFGDDEQKIRVYNSLIASLRNRAENLNILLN
                     PE"
     gene            9428..9583
                     /locus_tag="PLE39_16280"
     CDS             9428..9583
                     /locus_tag="PLE39_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNE69942.1"
                     /translation="MLFSPKEKGQGLVEYALILVLVAIVVIAALMILGPIIGNVFSKV
                     NSSLSNV"
BASE COUNT         2517 a         2361 c         2358 g         2639 t
ORIGIN      
        1 ttgcaataaa gtgtttagta tgtcttccat ctgcctgtcc tttgggaacg caatacctac
       61 gaaaaccaaa ttcgctttaa gtcaatcttt taaatatagt ttttaatatt tacagaggca
      121 atactgatga gatctttgtt gttgagaacg agagcagtcg ttgaaactgc tctcgttggt
      181 ggaccaatta ttacgaactc ttgtttttgt tgtacacatc aaacaaaacg gctaaaagaa
      241 gtaccatgcc ttttacgacc ttttgccagt caatacctac gccaagaatg gacatgccat
      301 tgttcaacac accgacaaga aaggctccaa gtactgcacc aataaccgta ccaataccgc
      361 cactcgcaga agcaccaccg atgaagcacg cggcgattac gtcaagctca aagccttcac
      421 ctgcatttgg tgtagaactg ttcaagcggg aggcgacaac catccccgcc aatgctgcaa
      481 gaacgcccat gtttacaaag gttaggaaca aaaggcgctg tgtgttgaca ccggaaagac
      541 gggctgcctt ctcatttcca cccatggcat agatacgacg cccaatcaca gtttgactgg
      601 tgaggaagga gtaaatcgtc accagcggga acatgattac aagcaagttg gggatgcctt
      661 tataagaagc catcaagtag caaaagccca tcactgcagc tgtaatcaag atctgtttca
      721 ctatgaagaa gtatgtggga gtgacttcaa atccgtattt ttgctgattg cgtcgcgttc
      781 gtatgtcaga aaagatcaag aggatggata ctagaacgcc tactacaatg gttgtaaggt
      841 gaaacccttc atagtggaaa aaatctggaa tatagccgcc actaatgttg cggaagcctt
      901 ccgggaacgg tccaatcgct tcgccgcgaa ccatgacaag ggtaaggccg cggaagatca
      961 acatgccagc cagcgtcacg atgaatgcag gaattttcag atacgccacc caatatccct
     1021 gccaggcgcc aattacaccg ccgatcgtaa gacaaatccc cattaccaat atccaattcc
     1081 attcccattt tacgatcatt acaccggcta tggagccgat gaaggcagct actgaaccga
     1141 ccgagagatc gatccatcct gaaacaatga tcaacagcat gcccagtgcc atgatgatca
     1201 cgtagctgtt ttggaggacg agattggtga tgttgatcgg tttcatcaga atgccatctg
     1261 tcatgaaata gaagaagatc atgatcacga tcaacgcgat caacatgccg tactcgcgga
     1321 tattgttctt gaaaaggttg gacaaagttt tcattgtgcg atagcctctt gttcaaacat
     1381 gatgtgcttc attacaagtt cttgcgacgc ttccttggcg ggcatttcac cgacgatctt
     1441 gccttcgcgc ataacataaa ttcggtcaca aacccctaat atttcgggta actcggagga
     1501 gattaaaata atcgcctttc cttcactggc aagttgattg atgatcgtgt aaatttcgta
     1561 tttggcgccc acgtcaatgc cgcgggtggg ctcatccaaa ataagaattt cagggttggc
     1621 aaaaagccac ttgcttagta ctactttttg ttgattgccg ccagagaggt tcagcgccaa
     1681 ttgaaggata ctggaacatt taatgtttaa tttatctcga tattcgcggg ttacggtcaa
     1741 ttcctttggc tcgttaatga caccagcatc tgagattgcg ttgaggttgg ataatgtgat
     1801 gttgtctttg atctcttcga taagtaccaa gccataggct tttctgtctt ccgtcgcata
     1861 tgcaattcca tttgcaatgg ctttgtcaat tgtgctaatg tcaatttcta ttccgtcttt
     1921 gaaggccttg ccgctaattc tggtgccgta ggcgcgccca aaaacgctca tcgcgagctc
     1981 tgttctgcca gctcccatca agccagatat tccaaccact tcaccacgat gaacttttat
     2041 gttcacattg ttgctgacct ttcggtcttc gtgttgtggg tgatatacat tccagtcttt
     2101 gatctcaaat acgacttcac cgattcttga ttcacgcggc ggaaaccggt gtgtgatatc
     2161 tcgaccgacc atgccgcgga taatgcgatc ttctgtgatg gtgtctttat gacaatccaa
     2221 tgtctcgatt gtggctccat ctcgcaggat tgtgatcgag tcggccactt tggatacttc
     2281 gctaagttta tgagatatca aaatagaagc aataccattc tctttgaatt gaaggagaag
     2341 ttcaaggagt ttctcgctgt cgttttcgtt gagagaggcg gtaggctcgt ctagaataag
     2401 aagtttgact tttttggcca gggctttggc aatttccacc aattgctgtt tgcctacacc
     2461 aatgtttgta attaatgtgt caggcgactc atgtaacccc actttgctca ataactcttt
     2521 tgttcttaag atcgactcat tccaatcgat aattccaaag tttgcacgct cgttgccaag
     2581 gaacaaattc tcagcaatgg aaaggtaggg aattaatgca agttcttgat gaataatcac
     2641 caatccaaca tcttccgact gggtaatatc atggaatctg cattccttgc cttcaaaaaa
     2701 tatctctcca tcataggaac cgtatggata gactccgctt agaaccttca tcagggtcga
     2761 ttttccagcc ccgttttccc caaccaaggc atggatttct ccctgcttga cagaaaaact
     2821 tacattatca agtgccttaa caccagggaa tgtctttgta atgttacgca tttccaaaat
     2881 gacatcagac atggttttct cgtccttgat ctatttaatg ataatgaaac gatattatgt
     2941 ggtaattcta attcaaaaat ccacaatttt ttcaatgagg ttggcttgtc ggagcttata
     3001 gcaagcctat ggtcttgttg acccctgtac gaggacggga tgccctcgta caggggtgtg
     3061 gaacttttac ttcagatctt caggtttgat gtaaccactg gcaatcagca gttcttcgta
     3121 gttagtgata tctacgctgt agggggtcaa cagataggag ggaacgacct tgacgccatt
     3181 gtcataggtc ttggtgtcgt tgatcggtac ttccttgccc tgcaaagcgg catccaccat
     3241 aatggaaact tgcttggcga gctcacgggt atccttgaac acggtgtagg cctgttcgcc
     3301 agcgatcatg gccttaacag aggcaacttc cgcatcctga ccggtgatga caggccaggg
     3361 ctggtcagct gtgccgtaac caacgccgcg caatgcagcg atgataccgc ggctcagacc
     3421 atcgtacggg gagagcactg catcaacgcg cttgtcagtg tagttggcgc tcagcaggtt
     3481 ttccatgcgg gactgggcta cagcaccatc ccagcggagg gtaccgacaa catccatgcc
     3541 catctggccg gactgaacaa cgagtttgcc ttcatcgata tagggctgga ggacggacat
     3601 ggcgccatcg tagaaataga aggcgttggt gtcgtcgggg gagccaccga agagttcgat
     3661 gttgaaagga ccagcctggt tcgcgaggtc gagaccttcg atgatctggg tgccttgaat
     3721 gacgcccacg ccgaagttgt cgaaggtggc gtagtaatct acgttttcgg tcttggagat
     3781 caggcggtca taagcgatga ccaaaacgcc ggcatcgcga gccttcttga gaacatcaga
     3841 gagagttgaa ccatcgatcg cggcaatgac gagtacgttg gcgcccttgg tgatcatgtt
     3901 ttcgatctgg gcaagctggt tcgggatgtc gttgtcagca aattgcaggt cggtttcata
     3961 accgagttct tcaaatgact tgaccatgct ctcgccgtcg gaaatccagc gggttgaggt
     4021 cttggtcggc atggaaatgc ctacgatctt gggctcggtg gaggaagcgg gagcttcttc
     4081 ggcagcaggc agatcttcag ctttgatata gccgctgtca acgaggagtt ctttgtagtt
     4141 ggtgatgtct acgctgtagg gggtcaacag ataggaggga acgaccttga cgccattgtt
     4201 gtaggtcttg gtgtcgttga tcggtacttc cttgccctgc aaagcggcat ccaccataat
     4261 ggaaacttgc ttggcgagct cacgggtatc cttgaacacg gtgtaggcct gttcgccagc
     4321 gatcatggcc ttaacagagg caacttccgc atcctgaccg gtgatgacag gccagggctg
     4381 gtcagctgtg ccgtaaccaa cgccgcgcaa tgcagcgatg ataccgcggc tcagaccatc
     4441 gtacggggag agcactgcat caacgcgctt gtcagtgtag ttggcgctca gcaggttttc
     4501 catgcgggac tgggctacag caccatccca gcggagggta ccgacaacat ccatgcccat
     4561 ctggccggac tgaacaacga gtttgccttc atcgatatag ggctggagga cggacatggc
     4621 gccatcgtag aaatagaagg cgttggtgtc gtcgggggag ccaccgaaga gttcgatgtt
     4681 gaaaggacca gcctggttcg cgaggtcgag accttcgatg atctgggtgc cttgaatgac
     4741 gcccacgccg aagttgtcga aggtggcgta gtaatctacg ttttcggtct tggagatcag
     4801 gcggtcataa gcgatgacca aaacgccggc atcgcgagcc ttcttgagaa catcagagag
     4861 agttgaacca tcgatcgcgg caatgacgag tacgttggcg cccttggtga tcatgttttc
     4921 gatctgggca agctggttcg ggatgtcgtt gtcagcaaat tgcaggtcgg tttcataacc
     4981 gagttcttca aatgacttga ccatgctctc gccgtcggaa atccagcggg ttgaggtctt
     5041 ggtcggcatg gaaatgccaa caagtttggc gtctgacgca ggagcggaag atccgccgcc
     5101 gcatgcagaa agtatcaggc tagccaacat cagcatggct agaaggttgt agaacaattt
     5161 cttagacatt tcttctcctt agttagtaag cttacgatgt tatattggcg taataattga
     5221 gcaagagcag ttttgaccat caagcagctg acgggtttgt ctcgccaagg aagaatgcac
     5281 ttcatacgcc aggcatcaga gtgcatatcc ttcgttttta tttacgagcc ccttgttcca
     5341 tcacctcctt ttcaatgaat gccgttcctg ttttgaatat ctgccaaagg gacggaggga
     5401 aatacggtca aacgcgtttg gaactttcac gtacaaccaa agttggttga atgaaccttg
     5461 attgcgctac gatcacttga ttattttgcc gagcctgtat catttccact aaactttgca
     5521 ctgcttgttc cccaagtaat tgcaagtcct gggaaattgt cgtcagggat gggtaaaaat
     5581 aagcggactc agcgatattg tcgaagccga taactgccaa ttgatctggg acacaaatgc
     5641 cccgacaaca ggtttctcgc aaaacgctta atgccatttg gtcgtttgca acaaagaccg
     5701 catccatatc tgggtatgat gcgagtaatt gtgtaaatgc ttgttcaccg ctggcagaag
     5761 accaatttcc ttcggcacaa tgctcttcca aaggatttaa ccctgccgcc ttcaaggtct
     5821 cgaaccaacc tcgtttgcgt tcatccgctt cccaccatgt aagcggacct gagatatgac
     5881 cgatcttctt tctgccgcac tccaataagt gttgaattgc cagaaccgca ccttgataat
     5941 tgtctgttgc cacactggat attcctgcac gtggtttcat ggcaagaaat aacacggggg
     6001 cagggatatt atgcatccgt tcttcaaccc aggcgtggtt atctccaatt tcaggggcta
     6061 cccaaatgat tccgtctact tgccgtgcca gtaatgagtc tattacttca tctattcgat
     6121 ttgtatcaaa attatccagt tctttcatca agagcatata gccaagttcg tcggctttat
     6181 ctgccactcc attaagcgtt cttgatggac caatatactt tagcccaaat gtgacaaccc
     6241 ccaacgtata actgcgttgt tgtatcaggc tgcgggccag tgtgcttggg tgatagccta
     6301 actgttccac aaccgtttcc actcgttggc gagtttcctc agagacataa gagaacttgt
     6361 tcatcacacg cgaaacagtt tgggtggaaa cacccgcagc ctgcgcgaca tctttgatgg
     6421 taactcgttt tcttctgtct gctattacca aggacttttg ccttcaagtt atgttatcga
     6481 taacatgtta tcgataacat aataacaaaa atgttatttg aagtcaagag agagtttttt
     6541 gaatttgtaa cactgaaatt tataacccat ttgggtgagg aactacgaca agtattcaag
     6601 tggaataaac aactttagag tgtttgacga acttaaaata aaaagcacag agagatttca
     6661 tccgtgcttt ttattttatg aaatttaaat gttctacttg ttgcttaatg cattccagcc
     6721 agcataatga gcttcgcttc ccaattccgc ttcgatgcga aggagttgat tgtattttgc
     6781 aactctatca gaacgggcag gagcaccggt tttgatctgg cctgtattga gtgcaacagc
     6841 caggtcagcg atggttgcat cttccgtctc gccggagcgg tgagaagtga ccgcgcgcca
     6901 gcctgcacgt tgacaaagat ccacagcttc gatggtttca gtgagggagc cgatctggtt
     6961 gaccttgacg agcaaagcat tggctgcgtt ttcagcaatg gctctacgga cacgttcagg
     7021 gtttgtcacc aacaggtcat caccaacgat ctggcattta tcgccaacgg tcttgatcat
     7081 caatttccat gcgtcccagt catcttgcgc gagaccatct tcgatggaaa cgatggggta
     7141 atccttgacc cacttggccc aaaattcaac catctgttcg ccggtcaatt ccttgccttc
     7201 tttgcgaagg ttatactttt tggtcttttc atcatagagt tcagatgcag cagggtcaag
     7261 cgcaattgca acctgctcac cacggcctgc tttaaatcct gctttttcaa tggcggcaag
     7321 gatcaattcg atggcttcgt tattggcctt gagagcgggg gcgtatccgc cttcgtcgcc
     7381 aacaagtgcg ccatatccct tctctttcaa aactgatttc aaggcatgat aaatttcagc
     7441 gccccaacgc aggccttcgg agaaggaagg tgcgccgaaa ggcatcacca tgaactcctg
     7501 catatcggtg ctttgccagc ttgtatgtgc gccgccgttc atgatgttca tcattggaac
     7561 agggagtaca tgtgcataca caccgccgag atagcgatac aaaggcatgc catatgaatt
     7621 tgctgcagct tttgcaacag caaggctgac gcccaaaatc gcatttgcgc cgagtttggc
     7681 tttgttgggt gtgccatcga gtgcaagtaa ttcattatca attgcttttt ggtctgatgc
     7741 atcccagccg aaaagggcct ctgaaatgac gccgtttaca ttggcaaccg ccttcgaaac
     7801 gcccttgccg aggtagcgtt tcttgtcgcc atcgcgcatt tccagtgctt catgctcacc
     7861 tgtggatgcg cctgaaggaa ctgctgcgcg ccccatggcg ccgtccatta aaaccacttc
     7921 cacttcaaca gtagggttgc cgcgtgagtc tagaatctca agagcggaga tgctttcaat
     7981 tgtagtgtcc attgctgact cctgaataag tatttgtgaa attacgtatc aagtataatc
     8041 gcattttgta tggagttgcc actttgattg cacaacaaga ttttgaccca cgtgaccgaa
     8101 agaaatatta tcttgaagaa acctttgcca gaagattttt gttgtcgctt gtacgggggg
     8161 tgtttcattc cgttatggta atgaaggtgg aagggcggga gaattttcca cttgatggtc
     8221 ctgttatcat cgccgccaac catgtgacca atttcgatgt gtttccgatg cagttttccg
     8281 tttcccgggc gatctgttac atgggcaaag ccgaattatt taacacgccc ctcgattcga
     8341 ttttgcgcat tctatgtgct ttcccggtct atcgcggcga aaaagatgaa tgggccatgc
     8401 gtcacgcgga aaaagttctg gcaaatgggc acaccctcgg aatgtttcca gaaggcaaac
     8461 gttcaaaagg aaaaggtctc ggggtggcaa aaactggcac ggccaggttg gcgattaaga
     8521 tgaattgccc gatcgtgccg atgaatgtga tcggtaccga cacatttttc aagcgatttc
     8581 catatcgttc aaacgtgacc gtgagactgc tgccgcctat tttgccgaat ccaaacgata
     8641 cgccgctttc tcttaccgac cgcctgatgt tcaccattgc cgagggtttg cccgaatcca
     8701 tgcgtggagt gtatgcagaa ctgccaaagg gatttggagc atgactggga tataatctcg
     8761 aaaaaaagca cgaaacggag ccaccatcat gacgcaagaa agttcgggga attccactcc
     8821 cgcccctaaa aagcttatcc cgtttctctg ttacgcaaaa gaaaaccagg tgatcgtacg
     8881 tgaattcagc caacgcttga aatctgaagg ttggatcgat ccatggttcg atgaagagga
     8941 tatcctgccc gggcaggtct ggcaggatag tgttgttcat gctgtcagac aatcacatgc
     9001 ggtgattgtt tttctttcta aagaagctgt tgcctccgaa ggattctttc aaaaagaatt
     9061 gaaacttgca ctggatacgg ctgccgaaaa accagaaggg acgattttta tcatccccat
     9121 tcgcattgac cattgcgagg tccctgagat gctggcgaaa taccaatacg tggattattt
     9181 cggcgacgat gaacaaaaga tacgggtcta caatagtttg atcgcatccc tgcggaatcg
     9241 cgcagaaaat ctcaacattc ttttgaaccc cgaataatcc gctattttac aataccgtat
     9301 tttttagtta acataactgt aaaggaaaac aacccaagcc cccttgaatt atttttcaga
     9361 ccgagtaaaa taagttcaat cctaggagac ctacctggga aattctgaag aaaggagaca
     9421 ctcaaccatg ttattttcac ccaaggaaaa aggtcagggt cttgttgaat acgctctaat
     9481 tctcgttttg gtcgccatcg tcgtgatcgc cgctctcatg atcctcggcc cgatcatcgg
     9541 caacgttttc agcaaagtaa actccagcct cagcaacgtg taattcacgt ctgtctgtca
     9601 aaaaatcccc gtcgcaaggc ggggattttt gttttttgct gggggaaaga agcgttttag
     9661 agacaattcc aaaagtggat tgctcaaaac gcataacccg ccgtgaaccg atttataata
     9721 cgcactatca tccactccat tgttggggaa gccccatgac ccgaaaactc cgcgtatttc
     9781 tctgccatgc ctcgcaagac aaacccgccg tgcgcggact atacaaacgc ctcgccgccg
     9841 aatcgtggat cgacccctgg ctcgacgaag agaaa
//
LOCUS       DAOQJC010000139         9873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_3618_length_9873_cov_9.228152, whole genome shotgun sequence.
ACCESSION   DAOQJC010000139 DAOQJC010000000
VERSION     DAOQJC010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3618_length_9873_cov_9.228152"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1685
                     /locus_tag="PLE39_16285"
     CDS             <1..1685
                     /locus_tag="PLE39_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNE69943.1"
                     /translation="EIYVGSQIPYQDRTQVARVMGWPEERVRIVGQLMGGGFGGKEDV
                     MGQIHTAMLANVTQRPVKLLFDRRESLLVHPKRHATQIRVKIGAKKNGRLVAAETELY
                     GDTGAYASLGEKVMTRATTHSAGPYDIPHVRADCYAMYTNNPPAGAFRGFGVTQSAFA
                     VESMMDKLAEELDLDPVELRRMNALHVGSVTNTGQELKESVGLLECIDRVESEMLKVA
                     GLPRGELFKPRVTPDTPHLVRSWGFASAYKNTGLGGGAPDISGADVELYADGTFQVRS
                     SAAELGQGLVTVMRLVVSEELAVAPEKVRVLVMDTDLTPNGGPTTASRQTFVTGNASR
                     YAAKTLREEITASMAEKFDVRPEQIHFEDGIVHVNGHSLSYADVYKEMTSMGQQPKVR
                     YEYEAPKTQPLGTGGDMHFAFSFGVQAAEVEVNKLTGEVKVLKVISANDVGMAVNPLG
                     LQGQVEGGVMMGLGNCLTEEFIVENGNVVTDQLARYRVPGIMLTPEITAIIVEHPISS
                     GPFGAKGVGEISSIPTTPAITNAIYNAVGVRFDKLPVDQEVIARELWEREEK"
     gene            1782..3050
                     /gene="thrC"
                     /locus_tag="PLE39_16290"
     CDS             1782..3050
                     /gene="thrC"
                     /locus_tag="PLE39_16290"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNE69944.1"
                     /translation="MKNFTGYKCSLCGTEYLPGQVTYTCPKDGGNLDVVIDFDSIRKK
                     YQPEDITSRTEPSLWKYLPLLPVSEPAGDSTPLHAAGGTPVFALPRLAEKLGLKHLWL
                     KDESRNPSASFKDRASAILVARAQEIKAEVVVTASTGNAGAALACMAAAVGQKAIIFA
                     PKSAPPAKVAQLLVFGAKVILVDGTYDDAFDLTVKAADEFGWYCRNTGYNPFTVEGKK
                     TAAFEIWEWWMEAHRDWHKKDSPLDNHPPLCIFVSVGDGNIISGIHKGFKDLLALGWI
                     PNMPRIIGVQAEGSAAIANAFNANTETITPVSATTLADSISVDLPRDGVRAVRAAKET
                     GGTYITVADDEIIKSIAELGKMGVFAEPAGATAYAGLVKATSLGVVGSDDPILVMNTG
                     SGLKDVRAAMQAVQSAPIIEPTLEAVKKLL"
     gene            3047..4216
                     /locus_tag="PLE39_16295"
     CDS             3047..4216
                     /locus_tag="PLE39_16295"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNE69945.1"
                     /translation="MKKKISQWSLPFRYAMGFVVFIAIVALLIYAREAVKMLVIAGFV
                     SYLISPAVILLMQKTKLSRTAAVNIVYFSALIFLVGVPATLTPIFFDEIKIVAQDILD
                     LSAEFSAVLSSPIQFGGMSFHFEQLGESLAHLQDTVLTPLPEEALALLETTSVNVLWF
                     LVIIVSVHLFMSQWPSIRDWLVDLAPEEYHHDMRELYKRLRNVWMAYLRGQIVLMLVV
                     GIVFTIAWLILGIPGALVLGVMAGLFTLVPDVGPFLAAAFAVGVALLEGSSWIPYSNV
                     IVAIIVFATYLVLINLKNFFVRPLIMGRSLHMNEALIFIAIMIATILEGIMGALLVVP
                     VLASVVVIMEYVRRRVLGLPPFEDDGEKRFVAPPEKIKARRPIKISTKEKRKEDL"
     gene            4213..5145
                     /locus_tag="PLE39_16300"
     CDS             4213..5145
                     /locus_tag="PLE39_16300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE69946.1"
                     /translation="MKITYSIIAPIYNEIDNLPELYRRVKEVMDSSGEPWELILVDDG
                     STDGSTDGIRALAEKDKTVRPVIFARNFGHQVAITAGWDYARGDAVVIIDADLQDPPE
                     VILDLAKKWKEGYEVVYAVRGEREGETWFKKFTAAMFYRIIYSITDVKIPVDTGDFRL
                     MDRKVVNVLKQMKERHRFPRGMSAWVGFKQIGVTYKRAARVAGVTKYPFKKMLRLALN
                     AITGFSYFPLQVATYFGFISAGISILAIPVVAILRLAGSHFFEGQTTTLVSVLFLGGV
                     QLISLGILGEYIGRLYDEAKGRPLYIVRDAPEDR"
     gene            5244..6608
                     /locus_tag="PLE39_16305"
     CDS             5244..6608
                     /locus_tag="PLE39_16305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNE69947.1"
                     /translation="MSLSFLRDRSFLREMLTIALPISFQQLINASLNLIDVLMVGQLG
                     ETSIAAMGLSNQVFFVFILLLFGLTSGMAIFTAQFWGKQEIEPIRKVLGMSILVSTLI
                     ALLFSLAATLMPQTVLGFYTTDPEVIDIGTRYLRIVGLSYIPVALATSYIATLRSIQF
                     VKITVVATTTALIFKTILGYMLIFGYGPVPALGVEGAAIGTASGWTLELVLLLVMVYT
                     QKTPLAANPLTFFSFDLSFFTRVLKTTLPALANEMFWSLGITTYNAIYAHIGTDAIAA
                     INVNATIEELGFVVFMGLGNACAVMVGNRIGAGKKEEAYETVRRIVILGVSAAWIVGI
                     IVVLMRDVVTNLYDLSPSGVHNVRMLMLMMACTLWLRMFNFSTFIGALRAGGDTRFAL
                     IMEICSIWLIGVPVAYTSAFVFNLPVYYVYLMVGLEELAKAFVSGWRVRSRKWIHDLV
                     NETS"
     gene            complement(6621..7043)
                     /locus_tag="PLE39_16310"
     CDS             complement(6621..7043)
                     /locus_tag="PLE39_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69948.1"
                     /translation="MVHALDEIRRTLKPNGILIDLRPVEDHWSVEVASQQNILTAGQL
                     TDMPIGKADDEAAFRAMREVEARGWFTREREEEFSFFYYWDTPSEMKEFMEKEWKDFE
                     KMEDKVYQTAKSLWSTADADAQMRVRVKMLITKWRNTT"
     gene            complement(7062..7358)
                     /locus_tag="PLE39_16315"
     CDS             complement(7062..7358)
                     /locus_tag="PLE39_16315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE69949.1"
                     /translation="MKPLKDPEEFEKKALHKFVDFSNAHVLEIGCGEGRLTWKYAAAS
                     TLTVGVDPDQNALRIARADTPYSLQEKVRFSRATARQLPFSKETFDIAVLAWSL"
     gene            complement(7355..7762)
                     /locus_tag="PLE39_16320"
     CDS             complement(7355..7762)
                     /locus_tag="PLE39_16320"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNE69950.1"
                     /translation="MAELKTKKTEASVEDFLNGVKDEATKKDCFEIVKLMKQATKADP
                     KMWGSSIVGFGTQHLKYASGRELEWMILGFSPRKQNLTLYLPGSLESYAGMLKKLGKH
                     TTGKGCLYIKSLKDVDTKVLKELVQQAVKTTKQ"
     gene            complement(7829..9511)
                     /locus_tag="PLE39_16325"
     CDS             complement(7829..9511)
                     /locus_tag="PLE39_16325"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNE69951.1"
                     /translation="MSDVYDLKSVKLPYLAGGALKLFAALVEGPLGGLLTPSLFESAG
                     VNWLRKQSFNDAPTHHPIHFTGELQTESSAVPENEWPQNASTTKGFQFKSVHELANAY
                     RSGKTTPEEVARKVLDAIKASDSTDLPLKAFIAVYNEDVMRQARQSTQRIKAGKALSI
                     FDGIPVAIKDEMDMAPYPTTVGTSFLGKNPAQEDATVVARLRNAGALLIGKANMHEIG
                     INVTGLNPHFGTTRNPYSPRHYTGGSSSGSATAVAAGLVSFAIGADGGGSIRIPASFC
                     GLVGLKATFGRISEYGAYPLDWSVAHIGPLAASASDAALAYALIAGPDPKDPNSLHQP
                     LPSLQGWDDLNLKGLKLGVYWPWFRHADAETVAACEAMLKEYEKMGCEIVEVTIPNLE
                     ANRIAHSVTITSEMAQAMNETYGKHHRDHGLDVRVNLALARQFSATDYLTSQRIRTRM
                     IQHFNDAFQLADVILTPTTGITAPEIKKGALPDGESDLTNTIEIMRFATAANMTGLPA
                     ITFPVGYTQKGLPIGLQVMGKAWDEKTLLRLALSAEQVVERKAPQVYFELLK"
BASE COUNT         2064 a         2440 c         2740 g         2629 t
ORIGIN      
        1 tggaaatcta tgtcggttcg caaattccgt atcaggaccg cacacaggtt gcgcgtgtga
       61 tgggctggcc cgaagagcgc gtgcgcatcg ttgggcagtt gatgggcggc ggcttcggcg
      121 gcaaagagga tgtcatgggg cagatccaca ccgccatgct tgccaatgtg acgcagcgtc
      181 ccgtgaaatt attgtttgac cgccgcgaaa gtttgctcgt tcatccgaaa cggcacgcca
      241 cgcagatccg cgtgaagatc ggcgcgaaga aaaatggtcg cctcgttgcg gcggagactg
      301 aactctatgg cgatacgggc gcgtatgctt cgctcggcga aaaagtgatg acccgtgcca
      361 ccacgcactc ggctggcccg tacgacattc cgcatgtccg cgccgattgc tacgccatgt
      421 acaccaacaa tccgcccgcg ggcgcattcc gcggatttgg cgtcacgcag tcggccttcg
      481 ctgttgaatc gatgatggac aaactcgccg aagaacttga cctcgatccc gttgaactgc
      541 gtcgtatgaa cgccttgcac gttggcagcg tcaccaacac gggacaggaa ttgaaagagt
      601 cagttggcct gctggaatgt attgatcggg tcgaaagtga aatgttgaag gttgcaggtt
      661 tgccgcgcgg ggaattgttc aagccgcgcg tgacacctga caccccgcac ctggtacgtt
      721 cgtggggctt tgcctccgcg tacaagaata cgggactcgg tggtggcgcg cctgacatct
      781 caggcgcgga tgtggaactt tatgccgatg gtaccttcca agtccgcagt tctgccgcag
      841 agttggggca gggactcgtc accgtcatgc gcttggtcgt ttctgaagaa ttggcggttg
      901 cgcctgaaaa agtccgtgtg cttgtcatgg ataccgacct gaccccgaac ggcggcccga
      961 ctacggcctc gcgtcagaca tttgtgacag gcaatgcctc gcgctacgcc gcgaagacac
     1021 tgcgcgaaga gatcaccgct tcgatggcgg aaaaattcga tgtgcgcccc gagcagattc
     1081 attttgaaga cggcattgtc catgtcaacg ggcattcgct ttcgtatgcc gatgtgtaca
     1141 aagaaatgac aagcatggga cagcagccca aagtccgcta tgaatatgaa gcgccgaaga
     1201 cccagccatt gggaacaggc ggcgacatgc acttcgcctt ctcgtttggt gtgcaagccg
     1261 ccgaagtgga agtgaataaa ctgactggcg aggtgaaggt tttgaaggtc atctccgcca
     1321 acgatgtggg tatggcggtt aatccgcttg gcttgcaggg acaggtcgaa ggcggcgtga
     1381 tgatgggcct cggcaactgc ctgaccgaag aattcatcgt cgaaaacggc aatgtggtca
     1441 cagatcaatt ggcgcgctac cgcgttcctg gcatcatgct caccccggag atcaccgcca
     1501 ttatcgttga gcatcccatt tcgtcgggtc cgttcggagc gaagggtgtc ggtgaaattt
     1561 ccagcatccc gaccacgcca gcgattacaa atgcaatcta caatgcggtg ggcgtcaggt
     1621 tcgataaatt gcctgtcgat caggaagtga tcgccagaga gttgtgggag cgggaagaga
     1681 agtaaattag taatcggtga ttattaactg gactctcatg gcgaaagcct gagagtcctt
     1741 ttctagtccg ggccttgtct atcgggtaaa atcatgccac tatgaaaaat ttcactggct
     1801 acaaatgctc actttgtgga acagagtacc tgcctgggca ggttacatac acttgtccga
     1861 aagatggcgg gaatctggat gttgtgatcg actttgattc cattcggaaa aaataccagc
     1921 ccgaagatat cacttcgcgg acagagccat ctctgtggaa gtatttaccg cttttgcctg
     1981 tgtctgaacc cgcaggtgat tccacgcctt tgcacgcagc gggtgggacg cctgtctttg
     2041 ccctgcctcg gctggcagaa aaactcggcc tgaaacatct ctggctgaaa gatgagtcgc
     2101 gtaatccgtc ggcttccttc aaggaccgtg cttccgccat tctggtggca cgggcacagg
     2161 agatcaaagc cgaagtggtt gtgaccgcat ccacaggcaa tgcaggcgcg gcactggcgt
     2221 gcatggcggc ggcggtaggc caaaaagcaa ttatctttgc tcccaagtct gcaccgcctg
     2281 caaaagtggc gcaattgctg gtttttgggg caaaggtcat tttggtggat ggaacatacg
     2341 acgacgcttt cgacctgacc gtgaaagccg ccgacgaatt tggctggtat tgccgcaaca
     2401 cgggctacaa tccctttaca gtggaaggga aaaagaccgc tgccttcgaa atttgggagt
     2461 ggtggatgga agcgcaccgc gactggcaca agaaagacag tccgctcgat aatcacccgc
     2521 cgctttgtat ttttgtttca gtgggtgacg gcaacatcat ctctggcatt cataagggat
     2581 tcaaagacct gctcgccctg ggttggattc ccaacatgcc gcgcatcatc ggtgtgcagg
     2641 cggaagggtc ggcggcgatc gcgaatgcgt tcaatgcaaa taccgaaacg attacgccag
     2701 tctctgccac aactttggca gacagcattt ccgtagacct gccgcgtgat ggggtgcgtg
     2761 cagttcgcgc cgccaaagag acgggcggca cctatatcac tgtggcggat gacgagatca
     2821 tcaagtccat tgcggaactg ggcaaaatgg gagtctttgc cgaacctgcc ggggccacgg
     2881 cttatgctgg tttggtaaaa gcgaccagcc tcggggtcgt tggaagcgat gatcccattt
     2941 tggtgatgaa tactggcagc ggtctcaagg atgtccgcgc ggcgatgcag gctgtccaat
     3001 ccgcgccgat cattgaacct accctcgaag cggtgaaaaa attattatga aaaagaaaat
     3061 cagccagtgg agtcttcctt tccgttatgc aatgggcttt gttgttttta ttgccattgt
     3121 tgccctgttg atctatgcgc gcgaagcggt gaagatgctg gtgatcgctg ggtttgtttc
     3181 atacctgatc agccctgctg tgattttgct gatgcaaaaa acaaaactct cgcggactgc
     3241 ggcggtgaat atcgtttatt tttcggcgct gatctttttg gtgggcgtgc ccgccacgtt
     3301 gacgccgatc tttttcgacg agatcaagat cgttgcccag gacatactcg acctttcggc
     3361 tgaatttagt gcggtgcttt ccagcccaat tcaatttggt ggaatgtctt ttcatttcga
     3421 acaattgggt gaaagcctcg cacatttgca agataccgtc ctgacccctc tgcccgaaga
     3481 agcactggct ttgctggaga ccacatcggt gaatgtgttg tggtttttgg tgatcattgt
     3541 cagtgtgcat ctttttatga gtcaatggcc gagcatccgt gattggctgg tggacctcgc
     3601 ccccgaggag taccaccatg atatgcgcga attgtacaaa cgcctccgca atgtgtggat
     3661 ggcatacctg cgtgggcaga tcgtgctgat gctggtggtg ggaattgtat tcacgatcgc
     3721 atggctgatc cttggcattc ctggtgcgtt ggtgttgggc gtaatggctg gtttgttcac
     3781 gcttgtgccc gatgtggggc catttcttgc ggcggctttc gcggtcggtg tggctctgtt
     3841 ggaagggtca agctggattc cgtactcgaa tgtgattgtg gcgatcattg tgtttgccac
     3901 gtatctggtg ctgatcaatt tgaagaactt ctttgtgcgc ccgctcatca tggggcgcag
     3961 tttgcacatg aatgaagcct tgatcttcat cgccatcatg atcgcaacga tcctggaggg
     4021 aattatgggc gctttgctgg tggtgcccgt actggcttct gtggttgtga tcatggagta
     4081 tgtgcggcgg cgcgtactgg gattgccgcc ctttgaagat gacggtgaaa aacgatttgt
     4141 cgctccgcct gagaaaataa aagcaaggcg gccgatcaaa atatccacaa aagaaaaacg
     4201 gaaagaagat ttatgaaaat cacttattcg atcattgctc ccatctataa cgaaattgat
     4261 aatcttcccg aactgtatcg tcgcgtaaaa gaggtgatgg attcttctgg cgagccgtgg
     4321 gaactcatcc ttgtagatga tggttccacc gacggttcca ctgatggtat tcgcgctctg
     4381 gctgaaaaag ataaaacggt tcgccccgtg atttttgcgc gtaattttgg tcatcaggtg
     4441 gccatcaccg caggctggga ttatgcccgc ggcgatgcgg tcgtcattat tgatgccgat
     4501 ttgcaggatc cgcccgaagt gatccttgat ttggcgaaaa aatggaagga aggttatgag
     4561 gtggtatatg ccgtacgcgg cgagcgcgaa ggcgaaacct ggttcaagaa attcaccgca
     4621 gccatgttct accgcatcat ttatagcatc accgatgtga agatccccgt ggatacaggc
     4681 gatttccgcc tcatggaccg caaagtggtc aatgtgctca agcagatgaa ggaacgccat
     4741 cggttcccgc gcggcatgtc agcatgggtg ggtttcaaac agatcggggt tacctacaaa
     4801 cgcgccgccc gtgtagcagg ggtgacgaaa tatcccttca agaaaatgct gcgtcttgcg
     4861 ttgaacgcca ttacgggctt ttcctatttc cctttgcagg tggccacgta tttcgggttc
     4921 atctcggcgg gtatttccat ccttgccatt cctgttgtgg ccattctgcg tctggcgggt
     4981 tcgcatttct ttgaaggcca aaccactaca ctggtttctg tgctttttct tggcggtgtg
     5041 cagttgatct cgttgggtat tctgggcgaa tacattggcc gcctgtatga tgaagccaaa
     5101 ggccgaccgt tgtatattgt ccgcgatgca ccggaagacc ggtaaatatc aatcgctgaa
     5161 tggcaatcag tcgttgaacg cttaaaacaa aacgttcaac gactttttca tcctttattc
     5221 ctcatccttt atccttttcc cccatgtccc tctcctttct ccgtgaccgt tccttcctgc
     5281 gcgaaatgct caccatcgcg ctccctattt catttcagca gcttatcaac gcttcgctca
     5341 atttgatcga tgtactcatg gtggggcagc ttggcgaaac ctccattgcc gccatgggat
     5401 tgtcgaatca ggtgttcttt gtttttattc tcctgctttt tggcctcacc agcggcatgg
     5461 caattttcac cgcccagttt tggggcaagc aggagatcga gccgatccgc aaagtgttgg
     5521 gtatgagcat ccttgtttcc accctcattg cgcttctatt ttctcttgcg gccacgctca
     5581 tgccgcagac cgttctgggc ttttacacaa ccgaccctga agtgatcgat attggcactc
     5641 gttaccttcg catcgtcggg ctttcctata ttcccgttgc acttgccact tcctatattg
     5701 ccactttgcg cagcattcaa ttcgtcaaga tcacggttgt tgccacaaca acagccctga
     5761 tctttaagac gattcttggt tatatgctga tctttggcta tggacctgtt cccgctcttg
     5821 gagtggaagg tgccgcaatc ggcaccgctt caggctggac cctggaactg gttctgctat
     5881 tggtcatggt ctacacccaa aaaacacccc tcgccgccaa cccgttgaca tttttctcct
     5941 tcgacctttc atttttcacc cgtgtcctca aaaccacact gcctgcgctt gccaatgaaa
     6001 tgttttggtc gctgggaatc accacctaca acgccatcta cgcccacatc ggcacagatg
     6061 cgatcgccgc gatcaatgtc aatgccacca ttgaagaact gggctttgtt gtttttatgg
     6121 gtctgggcaa tgcctgtgcg gtaatggttg gaaaccgtat cggtgcaggc aaaaaagaag
     6181 aagcctacga aactgtgcgg cgcattgtga tccttggggt gtctgccgcg tggattgtgg
     6241 gcattatcgt tgttttgatg cgtgatgttg ttacgaattt atacgatctt tcccccagcg
     6301 gtgtacacaa tgtgcgcatg ctgatgttga tgatggcctg cactttgtgg cttcgcatgt
     6361 tcaacttctc cacatttatt ggcgctttac gtgcaggtgg agatacccgt tttgccctca
     6421 tcatggagat ctgttccatc tggctgatcg gcgtgccggt ggcatacacc agtgcgtttg
     6481 tgttcaatct ccccgtatat tatgtttatc tgatggttgg gttggaggaa cttgccaaag
     6541 cttttgtcag tggctggcgc gtcaggtcac gaaaatggat tcatgacctg gtgaacgaaa
     6601 cctcgtaaag gcgcatccgc tcaggtagtg tttctccatt ttgtgatcaa cattttcaca
     6661 cggacccgca tctgtgcatc tgcatcggct gttgaccaga gcgatttcgc ggtttggtaa
     6721 actttgtctt ccattttttc aaagtctttc cattcctttt ccatgaactc cttcatctca
     6781 gacggagtat cccagtagta aaaaaaagaa aattcctctt cgcgttcccg cgtgaaccag
     6841 ccgcgtgcct ccacttcccg catggcgcgg aaggcggctt catcgtcggc tttgccgatg
     6901 ggcatgtcgg tcaactggcc tgcggtcaga atgttttgtt gggaggccac ttcaaccgac
     6961 cagtggtctt ccacggggcg caagtcgatc aggattccgt ttggtttgag ggttcttcga
     7021 atttcatcca gagcatggac catgctctcg tgttccattc atcagagcga ccaggctagg
     7081 acggcaatat caaaggtttc ttttgaaaaa gggagttggc gggcggttgc tcgagaaaaa
     7141 cggactttct cttgcaggct gtagggagtg tctgcgcggg cgatgcgcaa tgcattttgg
     7201 tcagggtcca ctccaacggt cagggtggag gcggcggcgt atttccatgt caacctgcct
     7261 tcgccgcatc cgatttccaa aacgtgcgca tttgaaaaat ccacaaactt gtggagagcc
     7321 tttttttcaa attcttcggg gtctttgagt ggtttcattg tttggtggtt ttcactgcct
     7381 gttgcacgag ttctttcaac acttttgtgt ccacatcttt gagcgatttg atatacaaac
     7441 aacctttacc tgttgtgtgc ttgcccaatt tcttaagcat gcctgcgtat gactcaaggc
     7501 tcccgggcag gtagagggtg aggttttgtt tgcggggtga gaaaccaagg atcatccatt
     7561 ccagttcgcg gccgctggcg tatttgaggt gttgggtgcc gaagccaaca atgcttgagc
     7621 cccacatttt ggggtctgcc tttgtggctt gtttcatcaa ttttacaatt tcaaaacagt
     7681 ccttttttgt ggcttcgtct ttgacgccgt ttaggaaatc ttccacgctt gcttcggtct
     7741 tttttgtttt tagctcagcc atgatggtct cctttttcta gtggataaac tggagtgatt
     7801 ttagcacatt tgttctttta gtgggtggct atttgagcaa ttcgaaatat acctgtgggg
     7861 ctttgcgttc tacaacttgt tcggcggaga gtgcaaggcg caataaggtt ttctcatccc
     7921 atgccttgcc catgacctgc agaccgatgg gcagtccttt ttgggtgtag ccaacgggga
     7981 aggtgatggc ggggaggccg gtcatgttgg cggcggtggc gaagcgcatg atctcgatgg
     8041 tgttggtcag gtccgattcg ccatcgggca gggcaccttt cttgatctct ggcgcggtga
     8101 tgcctgtggt cggtgtgagg atgacatcgg cgagttggaa ggcgtcgttg aaatgctgga
     8161 tcatgcgggt gcggatacgt tgggaggtga gatagtcggt ggctgaaaat tggcgggcga
     8221 gtgccaggtt cacgcgcaca tccagtccat ggtcgcgatg atgttttccg taggtctcat
     8281 tcatggcttg ggccatttcg ctggtgatgg ttacgctgtg ggcgatgcgg tttgcttcga
     8341 ggttggggat ggtgacttct acgatctcgc agcccatttt ttcatattcc ttgagcatgg
     8401 cctcgcaggc ggcgactgtt tctgcatccg catgacggaa ccagggccaa taaacgccaa
     8461 gcttgagccc tttgagattg agatcatccc agccttggag ggatggcagg ggttgatgca
     8521 atgagttcgg atctttgggg tcggggccag caatcaaggc gtaggcaagc gctgcatcgg
     8581 aggcggatgc agccaaaggt ccgatgtgag cgacgctcca atccagcggg tatgcgccgt
     8641 attcgctgat tcttccaaag gtggccttca atccgaccaa tccacaaaat gaagcgggaa
     8701 tgcggatcga tccgccgcca tctgcgccga tggcaaaaga gacaagccct gcggcaacgg
     8761 ctgtggcaga gccgcttgaa ctgccgcccg tgtaatgcct tgggctgtat gggttgcgtg
     8821 ttgtgccgaa atgcggattc aggcctgtga cgttgatgcc gatctcgtgc atgttggctt
     8881 tgccgatgag taatgcgcct gcatttcgca aacgggcgac gacggtcgca tcctcttggg
     8941 cagggttctt tcctaaaaag gatgtgccga ccgtggtggg gtagggagcc atgtccattt
     9001 catctttgat ggctacgggt atgccgtcga aaatgctgag cgctttgcca gctttgatgc
     9061 gctgtgtgga ttggcgtgcc tgccgcatca catcttcgtt gtacacagcg atgaatgcct
     9121 taagcggcag gtcggttgag tcgcttgcct tgatcgcgtc taaaactttt ctggcaactt
     9181 cttcaggtgt ggtttttccg ctgcggtagg cgtttgcgag ttcatgtacg cttttgaatt
     9241 gaaagccctt tgttgtggat gcattttgcg gccactcgtt ttcgggcaca gcagatgatt
     9301 cggtttgcag ttcccccgta aagtgaatcg gatggtgggt gggcgcatcg ttgaagcttt
     9361 gctttctcag ccagttgact cctgcgcttt caaacagact cggcgtgagc agtccgccca
     9421 gcggcccttc aaccagtgcc gcaaacaatt tcaacgcgcc gcccgccaaa tacggaagtt
     9481 tcactgactt aagatcgtaa acatcgctca tgcttcacct cgtgatgaga ttttcaaaaa
     9541 ctttgacccg tgaattttac cctgcttcat ttcgttgtat gcctgatggc gtgttttggt
     9601 cgtaagaatc caaaataata caggcgggct cgtcggagaa cccgcctttg tttaggcaat
     9661 gtgttgaaga tcgaacttcg gaaagtctgt cattttgctg gtgcgaaaaa ctttatttga
     9721 cctcttgcat aagtaaagtg ttgtagacca caaagtcacc aaggcactaa gtcactaagc
     9781 ttttttcttt gagtcttcga gactttgtgc ctcacctgca ctagcgtgcg gcgcaagtgt
     9841 tgtggtaaaa aatggactta tgcaagagtt cta
//
LOCUS       DAOQJC010000140         9872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_3619_length_9872_cov_8.788326, whole genome shotgun sequence.
ACCESSION   DAOQJC010000140 DAOQJC010000000
VERSION     DAOQJC010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9872
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3619_length_9872_cov_8.788326"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..1175
                     /locus_tag="PLE39_16330"
     CDS             246..1175
                     /locus_tag="PLE39_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69952.1"
                     /translation="MATKNQRTSRKTLDTKTLVTIVIAIIGVAGTIGAALITALFGFL
                     STKAQIELPISATQTAELKLATLTSEPSPSFTQTPASTSNMDWKNYLEIENNIQCNDI
                     VVLPQGLDYLSSSIPEKISQAISQGYADQSIYSWDKVPEADKDMYNAQRASITIDINN
                     IASEDWVEVTKSLTVSIGTEKEIPEKINAVRIGGGCGASGEIREFPPLNLATDFEQYT
                     DKTIYPEFDFFKLENGEFERFQIPFTCQAPGYYQLAINIPFNIRNKASEMQTTANVIC
                     PNEISLWFYDLMGNRVIGTSESYFWNGNGYVSK"
     gene            1338..2330
                     /locus_tag="PLE39_16335"
     CDS             1338..2330
                     /locus_tag="PLE39_16335"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE69953.1"
                     /translation="MTIDNRQFSSREQETINLLLQGKSNKQIALSLNVSISTVEFHLK
                     NIYRKLQVKSRTEAILRLGKPTGDDESRTQGQSIGSKVDKQRESIVDKISVDAENGGN
                     TNSHRRIPMKQSSYIVGGSLLFLIVLAAIAIFVKVYGHTLSAPPTTQAPLSTATMQPA
                     IVSTTAPATPESITATCPVETVDLKLFTNTNDGYCFLYPIENTAFPPNKVVINPNGIS
                     GGDFLPGDFYVLIQVEAASGRTTTQVANEKIAEAGGVLPITLTSILVDGKEAAVIDGL
                     PSQDSTRHVFIIANDRLYTFFFTPWDPRIEGFSQSLLKLYSTVTSTFHFLPTTP"
     assembly_gap    2538..2637
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2797..3261
                     /locus_tag="PLE39_16340"
     CDS             2797..3261
                     /locus_tag="PLE39_16340"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNE69954.1"
                     /translation="MTKTIVSPKSESAQNAASVKEFIASLDEQTLKDSKALIAMMQGI
                     SGSKPRLWNVGTIGFDSYHYKYDSGREGDSFIIGFYPRKGKITVYLMDGIARYSDLLA
                     RLGKHTTTGYCVYIKHLGDVELPILEQILQRSYENIKSQDGHINQILWKTEK"
     gene            3356..3865
                     /locus_tag="PLE39_16345"
     CDS             3356..3865
                     /locus_tag="PLE39_16345"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE69955.1"
                     /translation="MEFSTIYYELQQSTEMIRALLTGITQEEARVKPNAEAWSILEVV
                     CHLHDVEREDFREHLDCILHRQDEKWHEIDPQSWITSRKYNEQNFAEMQEKFFAERKK
                     SLEWLGGLQNPDWEKVYTTEYHAFSAGEMFSCWAAHDNLHIRQLVELRRLRLEHVTKP
                     YNIEYAGDW"
     gene            3981..4337
                     /locus_tag="PLE39_16350"
     CDS             3981..4337
                     /locus_tag="PLE39_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HNE69956.1"
                     /translation="MNNKSNARVYAMKFSGVYPLYVQKAERKSRTKEEVDKIICWLTG
                     YSEAELRQQIEQGNDFETFFAQAPALNPNRSLIKGVICGVRVEDIEDTLMQNVRYLDK
                     LIDELAKGKAMEKILR"
     gene            4585..5130
                     /locus_tag="PLE39_16355"
     CDS             4585..5130
                     /locus_tag="PLE39_16355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE69957.1"
                     /translation="MTTPTDRDLILQTRRGDADSRTAFGELVTRYQTSVFNVCYRILH
                     ERGEAEDLAQETFIRAYDRIHTFDIEREFGPWIRRVAANLCLNTIESQKVTSELDDER
                     DADSAQLPEAVQEVRERSEQIRHALASLPVHYRAVVELRHFQELSYEEIAAELNIPLS
                     DVKSHLFRARKILAEKLHASH"
     gene            5117..5665
                     /locus_tag="PLE39_16360"
     CDS             5117..5665
                     /locus_tag="PLE39_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69958.1"
                     /translation="MHLTDEQLNEYLDNETNERMQIETHLSSCGECAARLSALQALFT
                     ELDSLPEVALTKSLSEASPWGAAPFLPTRNLPAPLPKFLTLTVILQAAFALTAVIIAI
                     PFVANLLPAFEIPSLAESLAQLSTFNLQPATFQLPNFQPANLPTFNFQPATFEISSLL
                     MALAGVSVLWILGNGVFLRNQR"
     gene            5717..6208
                     /locus_tag="PLE39_16365"
     CDS             5717..6208
                     /locus_tag="PLE39_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69959.1"
                     /translation="MNEFLQIMVVILLFTIPLAAYFLVLAALFPNRTAKTQRVLTTMP
                     GRSFGVGLVNFLFFGLIALVLFSVAENAGSVVKTILMIPALVILAALTLALSFGLTGM
                     VNQLGERIFPEHLAWKRTALGTVILGLACLLPAIGWFLLFPYVGFVGIGAFILGLFQR
                     ETS"
     gene            6396..6914
                     /locus_tag="PLE39_16370"
     CDS             6396..6914
                     /locus_tag="PLE39_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69960.1"
                     /translation="MADISAIFFVLLILGIAFPAMLTAWWLLFPAIISRAQIRVDKTP
                     WGTFWMGLVILIAVSIPVAILLALPFGPAKFIGWLLLAAALAFSSIGSAGIAAHLGAR
                     LASHSGHSPLNGFIRGAVILELTAFFPVLGWFFIWPLALIMAFGATGFAFLNWMPREK
                     TIHAPEPIPSHA"
     assembly_gap    6939..7038
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7173..8654
                     /locus_tag="PLE39_16375"
     CDS             7173..8654
                     /locus_tag="PLE39_16375"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNE69961.1"
                     /translation="MTLSRRDFLKLASLVSASAALSSCAPVYRRITGDLPALPWTALG
                     AGDFLALNRLTFGPRVEERARFSEIGLQNYIEEQLDFESINDFSCDLQISTFKTLKME
                     ANEIEGASNELFDGYDRERAPRELRQATLLRQLYSKRQLYEVMTEFWSDHFNIFMDKE
                     WEFVLKPVDDREVIRKHALGSFRDLVWASAHSPAMLVYLDNQANEQSHPNENYARELM
                     ELHTLGVNGGYTQKDVMELARCLTGWKVKDHFWLGEFTFDKNIHDTGTKHVMGLSIQP
                     AGVEEAEQVIEMLVKHPSTARFVSTKLARRFLADEPPQEIIEKAVQTFLKTNGDIKSV
                     LRVILLDGLPLAQPKYKRPANFVLSAMRMLNVETDAAALHDYLVRMGQVAFNWPTPDG
                     YPDRSELWQGNLMPRWQFAFELIRNEIKNTKHNLQNLLDAASTGILQDDVDAMTSLLL
                     GAPLERITRDGLIDTVRAAGSTEEETLQIITASIIASPAFQWR"
     gene            8708..>9872
                     /locus_tag="PLE39_16380"
     CDS             8708..>9872
                     /locus_tag="PLE39_16380"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNE69962.1"
                     /translation="MLTQTWLPRFSFAPVNTAPRGDTLVVVFLRGAADVLNMVVPHGE
                     EAYYQLRPSLGIARPDDSKRKKEERVLDLDGFFGFHPNLGPLLEAWQSEQLAIIHACG
                     APDESRSHFKAMELMERGVDDERGPASGWIGRHLATLNTGNTSPLRAVGMGTRPQRSL
                     SGTVPVSALRSIADFHLGGDKHALQQMRASLETVYANDVLGLETLSIMDTLEKLDPST
                     YHSPLSTYPDTEFGLALKQTAMLIKAEVGLEVSAIDLGGWDTHFAQGSSTGLMPNLMK
                     DLAQGLAAFHADMADHQNKLTTVTMSEFGRRASENGSLGTDHGHGSMMMVLGGNVDGG
                     KVHGQWPGLEEGQLIGPGDLAVTTDYRDVLSEVLTKRLNNPATNEIFPDYQPKM"
BASE COUNT         2535 a         2431 c         2279 g         2427 t
ORIGIN      
        1 tggagcattt gaagacgatg atggattgga acaggatctt gaggaagaag gtcgtataga
       61 ggcggatatg cttgcgggag agggcagttg ggtatttttt ttcattttcc tgcccctcct
      121 gctgtcgatt gcataatgaa gtatgtaatc tataaggtgt tatatggcgc gctgtataag
      181 cggtggttgg cttgccccgt gcgcaacaac gtacttaatt caacttactc aagcagggtt
      241 tcataatggc tactaaaaat caaagaacat ctagaaaaac tcttgacacc aaaacgttgg
      301 ttacaatagt gattgcaatt attggtgttg caggcacaat aggtgcagcc ttaattacgg
      361 ctctgtttgg ttttttatca acaaaggcgc aaatagaatt accaattagt gcaacgcaaa
      421 cagcagaatt gaaattggca actttaacat ctgagccttc gccatctttc acacaaactc
      481 cagccagcac aagcaatatg gactggaaaa attatcttga aatagaaaac aatattcaat
      541 gcaatgatat tgtggtactg ccacaaggat tggattattt atccagcagc attcccgaaa
      601 aaatttcgca agccatatct caaggatatg cagaccaaag catttattct tgggataaag
      661 tgccagaggc ggacaaggat atgtataatg cacaacgagc aagtataact attgatataa
      721 ataatatcgc cagtgaggat tgggtagaag tcacaaaaag ccttacagta tcaattggga
      781 cagaaaaaga aattccagaa aaaataaatg ctgtaagaat tggcggcggt tgtggtgcca
      841 gcggtgaaat aagagaattt cctcctctaa accttgccac agactttgag cagtatacag
      901 acaaaactat ctatcctgag tttgatttct tcaaacttga aaatggggaa tttgaaagat
      961 tccaaattcc ttttacctgc caagcgccag gttattatca actcgctatc aatattcctt
     1021 ttaatattcg taacaaggca agtgaaatgc aaacaaccgc aaatgtaata tgtccaaacg
     1081 aaatctcgtt atggttttat gatttgatgg gaaatagagt tattggtaca agcgaaagtt
     1141 atttttggaa tggaaacggt tatgttagca aatagcaagc caacaaagcg tgcatctgac
     1201 gctggggact gcccgtaggg cgtgcgcaca tcccaagcag ttttctacgc ctgagcattt
     1261 ttctggttag acggcttcgc cgtccccgcc ccagcgcagg taacgcaaac ccttaggctc
     1321 atcaggtaaa taaaaaaatg acaattgata atcgtcagtt tagttcacgc gagcaggaaa
     1381 caataaacct tctcttgcag ggcaaaagta ataaacaaat tgctctgtca cttaatgttt
     1441 ccataagtac agtcgagttc catctaaaaa atatttatag aaaacttcag gtaaaatcca
     1501 gaaccgaagc gatcttgcga ctagggaaac ccacaggtga tgatgaatct agaacacaag
     1561 ggcaatccat aggcagcaaa gttgataaac aaagggaatc catagtagac aaaattagtg
     1621 tggacgccga aaatggcggc aacactaatt cacatcggag gattcctatg aaacaaagtt
     1681 cttacatcgt cggcggtagt ctgttatttt taattgtctt ggctgcgata gccatatttg
     1741 taaaagtgta tggacacact ttatctgccc cgccaacaac ccaagcgccg ctaagtactg
     1801 caaccatgca acctgcgata gtatctacaa cagcgcctgc gactcctgaa tcgataacgg
     1861 caacatgtcc tgttgaaact gtagatttaa aacttttcac aaacaccaat gatggttatt
     1921 gcttccttta tccaatagaa aatactgctt ttccgcccaa taaggttgtc attaatccaa
     1981 acggcatatc tggaggagac tttcttccag gggactttta tgtattaatc caagtggaag
     2041 ccgcttcagg tcgcactact acccaagtgg caaatgaaaa aattgcagag gcgggaggag
     2101 tcctccctat cacacttacc tcaatattgg tagacggaaa agaagcagct gttatagatg
     2161 gtcttcctag tcaggactct acccgtcatg ttttcattat tgccaatgac cgtttgtaca
     2221 cattcttctt cacaccttgg gacccacgga ttgagggatt ctctcagtcc ttattgaaat
     2281 tatattcaac ggtgacaagc acctttcact ttttgccaac cacgccataa gaacagataa
     2341 aaaaatatct gtcaaaacta atgcgcctaa caaagcgtgc atctgacgct ggggactgcc
     2401 gtaaggggtg agcacatctc aggcagtttt ctggctgggc ggcttcgctg tctgtgcagg
     2461 gtaaggtgct ggcgtcagaa aaaatataaa aatttatgac atcatttaaa tactatatag
     2521 ctgataaact aaaaaaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncag
     2641 cagaagattt caaccatacc agtgatgtca aataacaagc tgcccagcaa agcatggatc
     2701 tgacgtgcgt gggcgttctt ttgcaggttt ggggtctata tttccttctt gacgaaatag
     2761 aacatgggtt ctacaatttc gccaaggaga atatccatga caaaaacgat cgtttcgcct
     2821 aaaagcgagt ccgcgcaaaa tgcggcttcg gttaaggagt ttattgcttc actggatgag
     2881 caaaccctga aagatagcaa ggcgcttatt gcaatgatgc agggaattag tggaagcaaa
     2941 cccaggttgt ggaatgtagg tacgattggg tttgactcct atcattacaa atacgatagc
     3001 ggacgcgaag gcgacagttt tatcattggc ttttatccaa gaaaaggcaa gataaccgtc
     3061 tacctcatgg atggcatagc tcgttactct gacttactgg ccaggttagg caagcacacg
     3121 accactgggt actgcgttta tatcaagcac cttggcgatg tggagttgcc gatcctcgaa
     3181 caaatcctgc agagatcgta tgagaatata aagtcacagg atgggcatat caaccagata
     3241 ttgtggaaga ccgaaaaata ggcgcttccg ccatgcttta tcatgtgctc tgtgagcaaa
     3301 cctaaaaaag catgcatctg ggatgtcgtt ggatatatag aaatagtgag gccgtatgga
     3361 gttttcaaca atttactatg aacttcaaca aagtaccgag atgattcgcg ctttgttgac
     3421 tggcattaca caggaagagg cgcgtgtaaa gccgaacgcg gaggcatggt cgattcttga
     3481 agtggtatgc catttgcacg atgtggagcg tgaagatttt cgagaacatc ttgattgcat
     3541 tcttcaccgt caggatgaaa aatggcatga gattgacccc caaagctgga taacatcacg
     3601 gaaatataac gagcaaaatt ttgctgaaat gcaagagaag ttttttgctg aacgcaaaaa
     3661 gtcactcgaa tggctcggcg gactgcagaa cccagattgg gagaaggttt acacaacgga
     3721 gtatcatgca tttagcgcag gcgagatgtt ttcctgttgg gccgcccatg ataatcttca
     3781 catccgccag ttggtggaac tcagacgttt gaggctggaa catgtcacaa agccatataa
     3841 tattgaatac gcaggtgatt ggtaattgca cagcacaaaa taaggctcaa cttaagtttg
     3901 ttgtttgggt tgtgactgtt ggatcgtgct gaaagttcaa tgccttaatg gtaatgcaaa
     3961 gatgaataag gagttttact atgaataaca agtctaatgc tcgagtgtat gcaatgaagt
     4021 tctcaggtgt gtatccgctc tatgttcaaa aggcggaacg caaaagccgc acaaaagaag
     4081 aagttgacaa gatcatttgc tggttgactg ggtatagcga ggcggaatta cgtcagcaaa
     4141 ttgaacaggg aaacgatttt gaaacctttt ttgcgcaggc gccagccttg aatccgaacc
     4201 gctcgcttat taaaggcgtg atatgcggcg ttcgtgtgga ggatatcgaa gataccttga
     4261 tgcaaaatgt acgatatttg gataagctga tcgatgaact cgcaaaaggg aaggcgatgg
     4321 agaagatttt gagatagtag ggcggatagc aattgataac tccactgcaa aaaggcaatc
     4381 gaaccgcaaa gcacgcgaag cccgccaagg aaaagaaaga tctcaggcgc tttctcagga
     4441 aaattccttc tgaagattta agttgaaccg ttttgaattt tcttcgcgct ctttgcggcc
     4501 ttcgcggttg gaaaaggttt tgacagcgat tcaccctccc attctgcaac tttttcccgt
     4561 tgactcctgt attaaactct agccatgacc acgccaactg accgcgacct gatcctgcaa
     4621 acccgtcgcg gtgacgctga cagtcggacg gcatttggcg agcttgtcac acgctaccag
     4681 acgagcgtct tcaacgtctg ttaccgtatt ttgcacgagc gcggcgaagc cgaagacctt
     4741 gcacaggaga cctttatccg cgcctacgac cgcatccata cctttgatat cgaacgcgaa
     4801 ttcgggccgt ggattcggcg tgtggcggcc aacttgtgcc tgaataccat tgagtcgcag
     4861 aaggttacgt ctgagctcga cgatgaacgg gacgctgatt ctgcccaatt gcctgaggca
     4921 gttcaagagg tccgcgagag aagcgaacaa attcgccacg ccctcgcatc cctgcccgtg
     4981 cattatcgtg ccgtcgtcga attgcgtcat tttcaagaac tatcctacga agaaattgcc
     5041 gcagaattga acatcccatt gagcgacgtg aagagccatc tcttccgcgc ccgcaaaatt
     5101 ttggcggaga aactccatgc atctcactga cgagcaactc aacgaatatc tcgacaacga
     5161 aaccaacgag cgtatgcaga tcgaaacgca tctatcttca tgcggcgaat gcgcggcgcg
     5221 actttctgcg cttcaggcat tgttcaccga attggattcc ctgcccgaag tggcattgac
     5281 caaatccctt agcgaagcat ccccgtgggg cgccgcgccg ttcctgccga cccgaaacct
     5341 gccagcccca ctgccaaaat tcctcacgct gacagtgatc ctgcaggccg ctttcgcgtt
     5401 gaccgccgtc atcattgcca taccgttcgt tgcaaacctg ttgcccgcct ttgaaatacc
     5461 ttctctggca gaaagccttg cccaactttc aaccttcaac ctgcaacctg caaccttcca
     5521 acttcccaac tttcaacccg cgaacctgcc caccttcaat tttcaacctg caaccttcga
     5581 aatatccagc ctgttgatgg cgcttgcggg cgtttccgtg ctgtggatcc tgggcaacgg
     5641 tgtatttttg agaaaccaaa ggtagggata cggcgctgct gtatccgtga aaatagtaat
     5701 tcaacaagga gaattcatga acgagttcct gcaaattatg gttgtcatcc tgctgtttac
     5761 gattcctctc gctgcgtatt ttcttgtcct cgctgcgttg tttccgaacc gtactgccaa
     5821 aacccaacgc gtacttacaa ccatgcctgg gcgttccttc ggggtggggc tggtcaactt
     5881 tttgttcttc gggctgatcg cactggtgct tttttccgtt gctgaaaatg caggtagtgt
     5941 tgtgaaaacc attcttatga tccctgcctt ggtgatcctt gccgcgctga cgcttgccct
     6001 gagcttcggc ctgacgggca tggtcaacca acttggcgag cgcatcttcc ccgagcatct
     6061 cgcatggaaa cgaacggcat tgggaacggt gatcctgggt cttgcctgtc tcctgcctgc
     6121 catcggttgg ttcctgctgt ttccgtatgt gggttttgtc ggcattggtg catttatttt
     6181 gggattattc cagcgcgaaa caagctaatc aggtaaaaga aattttcaca ccgccgagag
     6241 acgcgggcac gcagagaaaa ttctgcgcac ccgcgtcttt gctgttaatc cactcgcaac
     6301 ttaccattca ccattcacta tttatttccc ctgcaacttt tccccaccca tccctgtaca
     6361 acctataact ctcaaaacct gaaaggaact ttcccatggc cgacatatcc gccatcttct
     6421 tcgtcctgtt gattctaggt attgccttcc cagccatgct caccgcgtgg tggctgttgt
     6481 tccctgcgat catttcccgc gcccaaatcc gcgtggataa aaccccctgg ggcacattct
     6541 ggatgggact tgttattttg atcgcggtca gcattcctgt tgccattttg cttgcgcttc
     6601 cttttggccc tgccaaattc atcgggtggt tgttgcttgc ggcggcattg gcattttcct
     6661 ccatcggctc ggcagggatt gcggctcatt tgggcgcacg cctcgccagc cacagcggac
     6721 actctccgct gaatggtttc atccgcggcg cagtcattct cgaactcacc gccttctttc
     6781 ctgtgctggg ttggttcttc atctggccgc ttgctctcat catggctttt ggcgccacag
     6841 ggtttgcctt tttgaattgg atgccgcgcg aaaaaaccat ccacgcacct gagcccatcc
     6901 catcgcacgc ataaaccaac cgcgaaaaat ctttcctcnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnncg cacctgagcc catcccatcg cacgcataaa ccaaccgcga
     7081 aaaatctttc ctctttcctc ccttccactt tccactcact actttccacc cactgataac
     7141 tgaccaccac taaccgatta ccaccaacca ccatgaccct ttcccgtaga gacttcctca
     7201 aacttgcctc ccttgtttct gccagcgcgg cgctttcctc ttgcgcaccc gtgtaccgca
     7261 gaattacagg tgacctcccc gccctgccgt ggacggcgct tggcgcaggt gatttccttg
     7321 ctttgaaccg tctcacgttc ggtccgcgtg tggaggagcg cgcgcgtttc agtgagatcg
     7381 gtttgcagaa ttacatcgaa gaacaactcg attttgaatc catcaatgac ttttcctgcg
     7441 atctccagat ttcaaccttc aaaaccctga aaatggaagc caacgagatc gaaggagcca
     7501 gcaatgaact cttcgatggc tatgatagag agcgcgctcc acgagaactg cgtcaggcca
     7561 cattgcttcg tcaactatac agcaaacggc agttgtatga agtgatgacc gaattctgga
     7621 gcgaccattt caacatcttc atggataaag aatgggagtt cgtcctcaaa cctgtggatg
     7681 accgcgaagt gattcgcaaa catgcgctcg gcagtttccg cgacctggtt tgggcatcag
     7741 cgcactcgcc agccatgctg gtctatctcg ataatcaggc caatgagcag agccatccca
     7801 acgaaaatta tgcacgagag ttgatggagc ttcacaccct cggtgtgaac ggcggctaca
     7861 ctcaaaaaga tgtgatggaa cttgcgcgtt gccttacagg ctggaaggtg aaggatcatt
     7921 tctggctggg tgaattcacc tttgacaaga acatccatga tacggggacg aaacatgtca
     7981 tgggtctttc gatccagcct gcgggtgtgg aagaagcgga gcaggtcatc gaaatgctgg
     8041 tgaagcatcc gagcacggcg cgcttcgttt ccaccaaact ggcccgccgc ttcctcgccg
     8101 atgagccgcc gcaggagatc atcgaaaaag ccgtgcaaac gttcctcaaa acgaacggcg
     8161 atatcaaatc ggttctgcga gtcattttgc tcgatggcct gccgcttgca cagccaaaat
     8221 acaaacgccc cgcaaatttt gtgttgtcgg ccatgcgcat gttgaatgtc gaaaccgatg
     8281 cggctgcgct ccacgattac ctcgtccgca tggggcaggt tgcgttcaac tggcccacac
     8341 ccgatggcta ccccgaccgc agcgaactgt ggcagggcaa cctcatgccg cgctggcaat
     8401 tcgcttttga attgatccgc aacgagatca aaaacacaaa acataattta caaaatttgt
     8461 tggatgcggc atctacgggc attttacaag acgatgtgga tgcgatgaca tccctgttat
     8521 tgggcgcccc gctggagcgc atcacccgcg acgggttgat tgataccgtc cgagcggcag
     8581 gctccaccga agaagagacc ctgcagatca tcaccgccag tatcatcgcc tctcctgcat
     8641 ttcagtggcg ctgattcgta ccgcaacatt catgttgcga tacggtactt ttaccaagga
     8701 tactttaatg cttactcaaa cctggcttcc ccgtttttct tttgcccccg tcaacaccgc
     8761 cccgcgcgga gataccctcg tcgtcgtttt tctccgcggt gcagccgatg tgttaaacat
     8821 ggtcgtgccg catggcgaag aggcgtatta tcaactgcgg ccttcacttg gaattgcaag
     8881 acccgatgac tccaaaagga agaaggagga aagggttttg gatttggacg gattttttgg
     8941 ttttcatcca aatctgggtc ctttgcttga agcctggcaa agtgaacagc ttgccatcat
     9001 ccatgcctgc ggcgcacccg atgaatcgcg cagccacttc aaagcgatgg aactcatgga
     9061 gcgcggcgtg gacgatgaac gcggccccgc ttcaggttgg atcgggcgtc atctggctac
     9121 gttgaacact ggcaacacat ctccgctgcg agcggtggga atgggcaccc gtccacagcg
     9181 cagcttgagc ggaacggttc ccgtctctgc cttgcgctcc attgctgatt tccatctcgg
     9241 cggcgacaag catgcccttc aacaaatgcg cgcttccctt gaaaccgtct acgccaatga
     9301 cgttttgggg ctggagactt tatctattat ggatacgttg gaaaaactgg atccatccac
     9361 ttaccactcg ccgctttcca cctaccccga tacagaattc ggccttgcgc tcaaacaaac
     9421 cgccatgctc atcaaagcgg aagtgggact cgaagtctca gccattgacc ttggcggctg
     9481 ggatacccat ttcgcgcaag gctcatccac tggcctgatg cccaacctga tgaaagacct
     9541 cgcgcaaggt ctcgccgcct tccatgccga tatggctgac catcaaaata aacttaccac
     9601 tgtcaccatg tctgagtttg gacggcgtgc ttctgaaaat ggcagcctcg gcacagacca
     9661 cggtcacggc agcatgatga tggttttagg cggcaatgtg gatggaggca aggtccacgg
     9721 gcagtggcct gggttggagg aaggtcaact gatcggccct ggtgatctgg cagtcaccac
     9781 cgattaccgc gacgtgctct ctgaagtttt gaccaaacgt ttgaataacc ctgcgacaaa
     9841 tgagattttc cccgactacc aaccgaagat gc
//
LOCUS       DAOQJC010000141         9756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_3686_length_9756_cov_9.385012, whole genome shotgun sequence.
ACCESSION   DAOQJC010000141 DAOQJC010000000
VERSION     DAOQJC010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9756
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3686_length_9756_cov_9.385012"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..768)
                     /locus_tag="PLE39_16385"
     CDS             complement(112..768)
                     /locus_tag="PLE39_16385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNE69963.1"
                     /translation="MEKVVLKASKRDVVGKQVKALRRNGQLPAVIYGRHVEPIAISLD
                     AHSTGLAFTKLTSSTLVTIDVDGTEYAALVREKQRNYIKGNLTHVDFLAVDLTEKITT
                     KVHLTFTGVAPAVKDYSAVLVHRMEQLEVECLPTDLPERILVDISSIKAIGNSIRVRD
                     IQLPANVAVLEDEDEIVMIATLAKEETTEAAAAAGTEAAAPALSVERGKKDEEGAEKK
                     "
     gene            complement(865..1386)
                     /locus_tag="PLE39_16390"
     CDS             complement(865..1386)
                     /locus_tag="PLE39_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69964.1"
                     /translation="MNEPSASSENDQVQVSNLSQVDVEMVQAELVRMHQSSAQEIQAE
                     DVELGMSAAAEVKAANATVRDSVVGLLQATDASMQDNLVGVARAETMSLNGGAGVVVA
                     GSVEFGNAYAGVVAGREVRGEKIESVILLSRNVHGNVTTVMDTRGALIAGLVGGLFAG
                     IMLLLGRMLFGRK"
     assembly_gap    1498..1597
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1610..2776)
                     /locus_tag="PLE39_16395"
     CDS             complement(1610..2776)
                     /locus_tag="PLE39_16395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE69965.1"
                     /translation="MPYFITSTILFVSALVIIYWIHNQYHLDIVVTSANPPADAPLFS
                     ICIPARNEENNISRCVESALAQDYPNIEVIVLDDRSTDATLTQLKEIAARDSRLHPIS
                     GSDLPAGWAGKPHALFQASAVAHGDWLCFVDADTFLEPGAISACYAKALETKADLFTT
                     MNQQVLGSFWEKVVMPLVMTALSVGFSPRKVNDPTRRDAVANGQFIMIRRAVYDAIGG
                     HEKVKDQIVEDKAISEQVKWNGHRLVVADGSRFIRTRMYTSLETMWEGWTKNIYLGLR
                     DHPAMLMLGAFGATLALIAALFLPIWPMLGLGWYLQGGGALAIWVILEALVVWGALLL
                     ARARVARKMFISAWYAWTTPLGAGVFAAMMLASAWKVISGQGVTWRGRKYHPAK"
     gene            complement(2820..5408)
                     /locus_tag="PLE39_16400"
     CDS             complement(2820..5408)
                     /locus_tag="PLE39_16400"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HNE69966.1"
                     /translation="MIPLRLRISGFLSYRDPVELDFDSFDLACISGHNGAGKSSLLDA
                     FTWVLFGEARGKSADIINLNSDVKAAEVALTFKYEGNVFRVQRTLPRNKSTLLEFQVL
                     DEGEQKADLESGTWKPLTEKTTRDTQARIEQTLRLDYDTFINASFFLQGKADEFTQKK
                     AGDRKAVLSSILGLEVWDEYKDRAADKRKIVEREVDEIDGRIAEIDSELAEEDARKAR
                     LAELETTLGQLSTSRAAQEAALANIRKNADLLNEQRKLTEALSTALERARIALLGLEK
                     RLTEKESERTAYTDLVHRAKEIESSYKAWQKTRKELESLDQIAMEFREHEKERTPLLE
                     QIATEKARLEEERRSLLVEQEEIENQSASMGDLTAEVEKARTALQDAEIRIAERTELD
                     SVRNDARERQAFLKAENDALKENMNSLKERMDSLDAADGATCPLCGQELTEKHRKATV
                     KQLEAEGKQKGDKFRANKSEMDQLEKTLADCTARLAKLTGAENDRLKFSNTISQLTER
                     IETLGVVAKEWEKSGKKRLAEVTKVLEKESFAADARKGLAKLDKELAKLGYDVATHEE
                     TRQQELELREVEEEYRKLESARELSKRIAKEIDELEEEIKKKQAEVSESEEAHRAAAQ
                     ALAESESHHPNLDDVERDLFALREDENRIRDQVGAARQKVEVLATLRGRKADYAVQRE
                     ELNQQIVRYKTLERAFGKDGVPALLIEQALPQIEQKANDLLDRLSDGQMSIRFVTQAE
                     YKDKKRDDLRETLDIQISDSAGQRDYEMYSGGEAFRVNFAIRLALSEILSQRKGARLQ
                     TLVIDEGFGSQDAQGRQRLIEAINLIKQDFAKILVITHLDELKDAFPNRIEIEKTERG
                     SAVKVV"
     gene            5412..5960
                     /locus_tag="PLE39_16405"
     CDS             5412..5960
                     /locus_tag="PLE39_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69967.1"
                     /translation="MMLDYNRPDMSETEAPRTRNRMRALAVILATIPCYCIGFIFLVL
                     APDVKPNATPTTTILTVTGTPSTPTQSVTPPIITNTITQIVIVTNTLTQTATPTYTPF
                     QANTVTLTFTLTPSITPTFTLTNTLTPSITPSVTLTSTPTNTLTITPSPTITLTPSLT
                     PTITLTPTPTNTPSPSPTAFGG"
     gene            6009..7097
                     /locus_tag="PLE39_16410"
     CDS             6009..7097
                     /locus_tag="PLE39_16410"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69968.1"
                     /translation="MSDILPFLKSIISVAGLSGHESPVADLIEKQWTPLVDEISRSKL
                     GSLHGLKKGTPSKGISKPEKSPRPSVLIATHMDAIGLMVTRIVDGFLYITNIGGIDHR
                     VLPGTPVTVHATGSGEELYGVIVMPPGNLLPEGKGEGAVNLKYLMVDTGLLPADVSKR
                     VRVGDRVSFNTEPVEMSGGLVSGHNLDNRASVAALTVCLEELQSKSHAWDVWALASVQ
                     EEVTYAGAYTSAFQLRPTIAVALDMTFGKGAGANGYQTFPLGKGLTLGIGPSVHPFLH
                     KRMKEVAERVEIPVKDEAMPEMSSTDADAMQLTAEGIPTVVVSIPQRYMHTPVEVVAL
                     KDIQRAGRLLAEFITSLEEDFLTKIVWD"
     gene            7103..8158
                     /locus_tag="PLE39_16415"
     CDS             7103..8158
                     /locus_tag="PLE39_16415"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNE69969.1"
                     /translation="MLSIGTTQIKLLEKLCNAMSVSGDEGEVRRIVLEEVKPFADEVK
                     VDALGSVLVRKHAAASSGKKPLRVLLDAHMDEVGFMIVSDEGDGFYQFEAVGGIDSRH
                     LVGKQVIVGKDHSLGVIGAKPIHLASSGEMEHSLSIDSLKIDLGLEGKAKVGDRATFA
                     TKFKKVGPSIMSKSIDDRIGVAILIELLKNAPRNIELCLAFSVQEEIGLRGAKVAAHY
                     FEPDLAIAVDSTPARDLPDYDGNENTKYNTQLGLGPAIYVANSSTIDDPRLVKFLQDT
                     AVKNKIPHQLRQPGGGGTDAGAIQLTRAGVPVVSVSVPHRYTHSPISVSRVDDWKNTL
                     NLLHTALKAMSPDLLKR"
     gene            8162..8881
                     /locus_tag="PLE39_16420"
     CDS             8162..8881
                     /locus_tag="PLE39_16420"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HNE69970.1"
                     /translation="MNGLIALLGSGEYLSVMDDVDRHLLANCGVTDRKPRVVCLPTAA
                     GREGDASVNRWSNMGVEHFTRLGADVQAVPVIDAESANDLNHASVVEDADIVYFSGGD
                     PSYLHRTMKDSLVWQAAQKAWARGAVYAGCSAGAMILGREIPDFRAMGMRSIPAFGSI
                     AAAFVMPHFDAIPLFGKPLVSALRGRLKAGEMMIGIDENTAIVGRQNEEWIVLGHAKA
                     HVFTKNESRSYSAGEKFSLGN"
     gene            8885..>9756
                     /locus_tag="PLE39_16425"
     CDS             8885..>9756
                     /locus_tag="PLE39_16425"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNE69971.1"
                     /translation="MKQLLKTLTESFGPSGYEDDIRKIVLKEVKPLADEVRVDALGNV
                     IARKKPASGAKNPKKIMFAAHMDEIGVIVSHIDKNGFVRFTNIGGTFGRYTLGARVRF
                     LNGVSGVVGYDRLEQMDHWLPIDKMYIDVGATSKKDCPVKVGDVAAFERPFVEMGNRF
                     VAKSLDDRSGVVAVIEAMRAVKSTPNDLYFVFTTQEEVGTRGAGTAAYGIDPDIGIAV
                     DVTPTGDTPSSLKMVMELGKGPCVKFRDMGMLSDPRVVDWMLQTAEKAKIPHQREVLL
                     VGSTDAREIQVSRAG"
BASE COUNT         2207 a         2654 c         2454 g         2341 t
ORIGIN      
        1 agtcatgcta tatccataag aatgtgacca agttccgtca gacattctga tgcacacaaa
       61 acaaaaaacc cgtcatccgt tggatggcgg gtttttgatt cacacagatt cttacttctt
      121 ctctgcgcct tcttcgtcct tcttgccacg ttccacgctg agggcgggag cggcggcttc
      181 ggtaccagcg gcagcagcgg cttcggtggt ttcttccttg gccagggtgg cgatcatcac
      241 gatttcatct tcatcttcca acacagcaac gttggcggga agctgaatat cgcggacgcg
      301 gatactgttg ccaatggcct tgatggatga aatatccaca aggatacgtt cgggcaggtc
      361 ggtgggcaaa cattccactt cgagctgttc catgcggtgt acgagcacgg cgctgtaatc
      421 cttcaccgcg ggggccacac cggtaaaggt gaggtgaacc ttggtggtga tcttttcggt
      481 cagatccaca gcgaggaagt caacgtgtgt gaggtttccc ttgatgtaat tgcgctgctt
      541 ttcgcgcacg agggcggcgt attcggtgcc gtccacatca atcgtcacca atgtggatga
      601 ggtcaacttg gtgaatgcaa gtccggtgct gtgagcatcc agtgaaatgg caatcggttc
      661 cacgtgacgg ccatagatca ccgcaggcaa ttgaccattg cggcgcaggg ccttcacctg
      721 cttgccaacc acatcacgtt ttgaagcctt caatactact ttttccatct ttactccttg
      781 ggcgcctctc accgcgatcg cgcggttacc ttcaccctat taaatgaatt tctgcaaagc
      841 ggcttattcc gctgaacatt caaattattt tcggccaaaa agcatacggc ccagcaacaa
      901 catgattccc gcgaacaatc cgccgaccaa acctgcgatc aaagcgccgc gcgtgtccat
      961 cacagtggtg acattgccat gtacattccg cgaaagtagg atgaccgatt cgatcttctc
     1021 accgcggact tcacgtcctg ccacaacgcc agcataggca ttgccaaatt cgacgcttcc
     1081 tgccacgacc acgcctgccc cgccattcaa agacatggtc tcagcccggg caactcctac
     1141 gagattatcc tgcatgcttg catctgttgc ctgaagcaag ccaacaaccg aatcacggac
     1201 cgtggcattg gcagccttca cctctgccgc ggcgctcatt cccaactcca catcttctgc
     1261 ctgaatttct tgtgcagagc tctgatgcat ccgcaccaat tcagcctgaa ccatctcaac
     1321 atccacttgc gagaggttgc ttacttgaac ctgatcattt tcggagcttg cagagggttc
     1381 gttcatggca tactcttttg tttttggcgg aaaaacgcgc ctcctcgcgg aggcgcaggg
     1441 aacaacggct tggattttat acgcgggaag aggattcgtc aaggatatgc gcaaaagnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaag ccagatcact tacttggcag
     1621 ggtgatattt tctgccgcgc caggtgaccc cctgccctga aatgaccttc caggcggagg
     1681 ccagcatcat ggcggcaaag acacctgcac cgagaggggt tgtccatgca taccaggcgg
     1741 agatgaacat tttgcgggcc acccgagcac gtgccagcaa caaggcaccc caaacaacca
     1801 atgcctcaag aataacccaa atcgccaatg ctcccccgcc ttgcagatac caacccaaac
     1861 ccaacattgg ccagatggga agaaacaagg ctgcgatcag ggcaagcgtg gcaccaaatg
     1921 cgccgagcat taacatggca ggatggtcgc gcaagccaag gtagatattt tttgtccagc
     1981 cttcccacat ggtttcaagg gaagtgtaca tgcgtgttcg gatgaaacgc gaaccatccg
     2041 caacgaccag acgatgacca ttccatttga cctgttcaga aatggcttta tcttccacga
     2101 tctggtcttt cactttttca tgcccgccaa tggcatcata cactgcacga cggatcatga
     2161 tgaactgtcc attcgcaacg gcatcgcggc gggttgggtc gttgaccttg cgcggagaaa
     2221 atcccactga gagtgcggtc atgaccagag gcatgaccac cttttcccaa aaagaaccaa
     2281 gcacttgttg gttcattgtg gtaaacaggt ctgctttggt ttcgagggct tttgcataac
     2341 atgctgaaat ggcgccaggt tcaagaaagg tatcggcatc tacaaagcac agccagtcgc
     2401 cgtgcgccac tgccgaggct tggaacaatg catggggttt acctgcccag cctgcgggta
     2461 ggtcagatcc gctgattggg tggagacggg aatcacgggc ggcaatttct tttaattgag
     2521 tcagagtcgc atcggtggag cggtcatcca aaacaatgac ttcgatgttc ggataatcct
     2581 gcgcaagtgc ggattcaaca cacctgctga tattgttttc ttcattgcgg gcagggatgc
     2641 agatcgagaa cagcggcgca tctgcgggag gattggcgga tgtaacaaca atatcaagat
     2701 gatactgatt gtggatccag taaatgatga caagtgctga gacaaagagg atggttgatg
     2761 tgatgaaata aggcatggga aaacatggaa agtagaaagt ggaaagtggg ttttattttc
     2821 tatacgactt tcacggcaga accccgctcg gtcttttcaa tttcgatgcg gtttggaaag
     2881 gcatctttga gttcatcaag atgggtgatg acgagaattt tggcaaaatc ttgtttgatg
     2941 aggttgatgg cttcgatcaa acgctggcgg ccttgcgcat cctgcgaccc aaaaccttca
     3001 tcaatgacga gggtttgcag gcgggcgcct ttgcgttggg aaaggatttc agaaagcgcc
     3061 agacggatgg cgaagttaac acggaaggct tcaccgcccg aatacatttc gtaatcgcgc
     3121 tggcctgccg aatcgctgat ctgaatatcg agcgtttctc tgaggtcgtc tcgttttttg
     3181 tctttatatt cggcctgtgt cacaaatcgg atggacattt gcccgtcact caagcggtcg
     3241 agcaggtcgt tcgctttttg ttcgatctgc ggaagcgcct gctcgatcaa caaggcgggc
     3301 acaccatcct tgccaaaggc acgttccaaa gttttgtagc ggacgatctg ctgattaagt
     3361 tcttcacgct gaacggcgta atcggccttg cgcccgcgga gtgtggcaag cacctccact
     3421 ttttgccgcg ccgcaccgac ctggtcacga attcggtttt cgtcttcgcg caatgcgaac
     3481 aagtcgcgtt cgacatcatc caaattcggg tgatgcgatt cggactcagc caaagcctgt
     3541 gcagccgcgc gatgggcttc ttcactttcc gacacttccg cttgcttctt ttttatttcc
     3601 tcttccagtt cgtcgatttc ttttgcgatc cgtttgctta attcgcgcgc cgattcgagc
     3661 ttgcggtatt cctcttccac ttctcgcaac tcgagttcct gctgacgggt ttcttcatgg
     3721 gtggcaacat cgtacccaag tttggcgagt tctttatcca gtttcgccag acctttgcgc
     3781 gcatcggcag caaaggattc tttttccaaa actttagtga cttcggccag ccgttttttg
     3841 ccagatttct cccattcttt tgcaaccaca cccaaagttt cgatgcgttc tgtaagttgg
     3901 gaaatggtat tggaaaattt cagacggtcg ttctctgcgc cagtgagctt tgcaagacgg
     3961 gccgtgcaat cggccagcgt tttctcaagc tggtccattt cgcttttatt ggcgcggaat
     4021 ttatcgcctt tttgtttacc ttcggcttcc aattgtttga ccgtggcctt gcgatgtttt
     4081 tcggtcaatt cctgtccgca cagggggcag gtcgcgccat cggcggcatc aagggaatcc
     4141 atgcgttcct tcaggctgtt catgttttcc ttgagcgcgt cattctctgc tttcaagaaa
     4201 gcctgacgct cccgcgcatc gtttcgtaca gaatccaatt cggtccgctc tgcgattcgg
     4261 atttccgcat cttgcagggc ggtgcgggcc ttttccactt cggcggtcag gtcacccatt
     4321 gaagcgcttt gattttcgat ctcctcctgc tcaaccagca aacttcgtct ttcttcttca
     4381 agacgtgcct tttcagtggc gatctgctcg agcagtggtg ttcgttcttt ttcatgctct
     4441 cggaattcca tcgcaatttg atcgagcgat tccaattcct tgcgcgtttt ttgccaggct
     4501 ttgtaactgg actcgatctc ttttgcgcgg tgaaccaggt ctgtgtatgc ggtgcgttcg
     4561 gattctttct ccgtgagccg tttttccagg cccaaaagtg ctatgcgtgc gcgctccaaa
     4621 gcagtggaca gggcctcagt cagcttacgc tgctcattca gcaggtcggc attttttctg
     4681 atattggcca acgccgcctc ttgtgcggca cgtgaagtgg aaagctggcc aagggtggtt
     4741 tcaagctctg cgagccgcgc tttgcgcgca tcctcttcgg ccaattctga atcgatctcg
     4801 gcgatgcggc cgtcaatttc atccacctcg cgttcgacga tctttctttt atcggcggca
     4861 cggtctttgt attcatccca cacttccaac ccgaggatgg aactcaacac ggctttgcgg
     4921 tcacccgcct ttttttgagt gaattcatcg gccttgccct gcaaaaagaa ggaggcattg
     4981 ataaaggtat cgtaatccag tcgcagtgtc tgttcaatgc gggcctgcgt atcgcgggtc
     5041 gttttctcag tgagcggctt ccacgttccg ctttcaaggt ctgccttctg ttcgccctcg
     5101 tccaaaacct gaaactccag cagggtgctt ttgttgcgcg gcagtgtcct ttgcacacgg
     5161 aacacattgc cttcgtactt gaaggtcaac gccacttccg cagctttgac atccgagttc
     5221 aagttgatga tatcggcgct ttttccgcgt gcctcgccga acaaaaccca ggtgaacgca
     5281 tccaacaggg atgatttacc cgcgccgtta tgacctgata tacaagccag gtcgaacgag
     5341 tcaaaatcca attcaacagg gtcgcgatat gaaagaaagc ctgaaattcg aagacgtaga
     5401 ggaatcataa gatgatgttg gattataatc gacccgatat gtctgaaacc gaagcacccc
     5461 gcactcgtaa ccgaatgaga gcccttgcgg tcatccttgc caccattccc tgctattgca
     5521 tcgggtttat ctttcttgta ctggccccag atgtaaaacc gaacgccact cccaccacca
     5581 ccatcctcac ggtcactggc acgccttcca ctcccactca aagcgtcacc cctcccatca
     5641 tcacaaatac catcacacaa atcgtcattg tcaccaacac attgacacaa acagccacac
     5701 caacctacac ccccttccag gccaacacgg ttaccttaac gttcacactc accccatcca
     5761 ttacacccac tttcaccctg accaacacac tcacccccag cattaccccc agtgtaactc
     5821 tcacctccac cccaaccaac acactcacca tcaccccctc tcccaccatc acgttaacac
     5881 catctctcac gccaacgatc acactgaccc caaccccaac caacacccca tctccatctc
     5941 ccactgcgtt tgggggataa cgcaatgcac gcaacacaaa attctttgaa agccattagg
     6001 gtttaccaat gagcgatatt ctcccatttc taaaatcaat catttctgtc gcaggccttt
     6061 caggacatga atcccccgta gcggacctca tcgaaaagca gtggactccg ctggtggatg
     6121 agatcagccg aagcaaactc ggttctttgc atggactgaa aaagggtacg ccttcgaaag
     6181 gcatttccaa acccgaaaaa tccccccgcc cttccgtact catcgccacc cacatggatg
     6241 ccatcggcct gatggtcacc cgcattgtag atggattttt gtacatcacc aatatcggcg
     6301 gcattgacca tcgtgttctg ccaggcacac ccgtcaccgt ccatgccacg gggagcggcg
     6361 aagaactgta tggggttatc gtcatgccgc ctggcaattt actgcccgaa ggcaagggcg
     6421 aaggcgctgt caacttgaaa tacctcatgg tggatacagg cctcctgcct gccgacgtct
     6481 cgaagcgagt ccgcgttggg gatcgagtct cctttaacac ggaaccagtt gaaatgtcag
     6541 gcggactcgt gagcgggcac aatcttgata accgcgcttc cgtcgccgcg cttaccgtct
     6601 gccttgaaga acttcaatcc aaatcacatg cgtgggatgt gtgggcgctg gcctctgtgc
     6661 aagaggaagt gacctacgca ggcgcgtaca catcggcatt tcaattgcgc cccaccattg
     6721 cagtggcatt ggatatgacc tttgggaaag gtgcgggcgc gaacggatat caaaccttcc
     6781 cgctgggcaa gggactgaca ctcggcatcg gcccgagtgt ccatccgttt ttgcataaac
     6841 gcatgaagga agtggccgaa agggtggaaa tccccgttaa ggatgaagcc atgccagaaa
     6901 tgtcatccac cgatgcagat gccatgcaat tgacggcgga aggcatccct acggtggtgg
     6961 tcagcattcc gcaacgatac atgcataccc ctgtggaggt cgttgcgctg aaagatattc
     7021 agcgtgctgg ccgtttgctt gccgaattta tcacctcgct tgaagaagat tttcttacca
     7081 aaatcgtgtg ggattaatca atatgctttc aattggcaca acccaaatca aactgcttga
     7141 aaaactatgc aatgccatgt ctgtttcagg ggacgaaggc gaagttcgcc gcattgtcct
     7201 tgaagaagtg aaaccctttg ccgatgaagt caaagtggat gcgctcggca gtgtgctggt
     7261 gcgcaaacat gcggcggcct cttctggcaa aaaaccgctg cgggtgctgc tcgatgccca
     7321 catggacgaa gtgggtttta tgatcgtcag cgatgaaggc gatggctttt accaattcga
     7381 agcggtcggc ggcattgatt cgcgtcatct cgtcggcaaa caggtcatcg tcggcaagga
     7441 tcacagcctg ggcgtgatcg gcgcaaaacc cattcacctc gcctccagcg gagaaatgga
     7501 acattccctc agcattgact cattgaagat cgatctcggc ctcgaaggca aagccaaagt
     7561 cggcgaccgt gccacctttg ccacaaagtt caaaaaggtc gggccttcca tcatgtccaa
     7621 atccattgat gaccgcatcg gcgtggcgat cttgatcgag ttgttgaaaa acgcaccaag
     7681 gaacattgag ctttgccttg cgttcagtgt gcaggaagag atcggcttgc gcggggcaaa
     7741 agtggcggcg cattatttcg aacctgatct cgccattgcc gtcgattcca cgcctgcgcg
     7801 cgacctgccc gattatgatg gaaatgaaaa cacaaaatac aacacgcagc ttgggcttgg
     7861 ccctgccatt tatgtggcga attcatccac aattgacgac ccgcgccttg taaaatttct
     7921 tcaagacacg gcggtcaaaa acaagattcc ccatcaactg cgccagcctg gcggtggcgg
     7981 caccgatgca ggtgcgatcc aattgacacg cgcaggcgtg cctgtggttt cggtttcagt
     8041 gccgcatcgc tatacccatt cccccatcag cgtttcacgt gtggacgatt ggaagaatac
     8101 gttaaatctg ctgcacactg cgttgaaagc catgtcacct gatctactta aaaggtaaaa
     8161 gatgaacgga ctgatcgctt tattgggttc tggtgaatat ctttcggtca tggatgatgt
     8221 tgatcgtcat ctgctggcaa actgcggagt gaccgaccgc aagccgcgtg tggtttgtct
     8281 gccaaccgct gcgggcaggg aaggcgacgc aagcgtaaac cgctggtcga acatgggcgt
     8341 ggagcatttc acccgcctcg gggctgacgt tcaagctgtt cctgtcatcg acgcagaatc
     8401 agccaatgac ttgaaccatg cctcggttgt ggaagatgcg gatatcgtct acttttcagg
     8461 cggcgatccc tcgtatctgc accgcaccat gaaagatagt ctcgtctggc aggcggcgca
     8521 aaaagcctgg gcgcgtggag ctgtgtatgc gggctgttcg gcgggcgcaa tgatccttgg
     8581 gcgggagatc cccgatttcc gcgccatggg gatgcgttcc attccagcct ttggttcgat
     8641 cgcagccgcg ttcgtcatgc cgcattttga cgccattcca ttgttcggca agccgctcgt
     8701 ctctgccctg cgcggacgtt tgaaagcggg cgaaatgatg atcggcattg atgaaaacac
     8761 cgccatcgtc ggcaggcaaa acgaagaatg gatcgtgctt gggcatgcca aagcgcatgt
     8821 gttcacaaag aatgaaagcc gcagctacag tgctggcgaa aaattttccc taggaaatta
     8881 accgatgaaa caattgctca aaacactcac tgaatccttt ggtccatcag gctatgaaga
     8941 tgatatccgc aagatcgtat tgaaagaggt gaaaccgctc gccgatgaag tgcgcgtgga
     9001 tgcgctcggc aatgtgatcg cgcgcaaaaa gcccgccagc ggggcgaaga atcccaaaaa
     9061 gatcatgttc gccgcgcaca tggacgaaat cggcgtcatt gtcagccata ttgataaaaa
     9121 cggttttgtg cgttttacca atatcggcgg cacgtttggc cgttatacgc tcggggcgcg
     9181 tgtccgcttt ttgaatgggg tctcaggggt tgtagggtac gaccgtttgg agcagatgga
     9241 tcattggctg cccatcgaca aaatgtatat tgatgtgggt gcaaccagca aaaaggattg
     9301 ccctgtcaaa gtgggcgatg tggctgcatt cgaacgtcca ttcgttgaaa tgggcaaccg
     9361 cttcgttgcc aaatcactgg atgaccgttc aggcgtggtg gcagtgatcg aagcaatgcg
     9421 cgcggtcaaa tccacaccca atgacctgta tttcgtgttc accacgcagg aagaagtcgg
     9481 cacacgcggc gcaggtaccg ccgcctacgg cattgacccc gatatcggta tcgctgtgga
     9541 tgtaaccccc actggcgata cgcccagttc gctcaagatg gtgatggagc ttggaaaggg
     9601 cccctgtgta aaattccgcg acatgggtat gttatctgac ccgcgggtgg tggattggat
     9661 gctccaaact gctgaaaagg cgaagatccc ccatcaacgt gaggttctgt tggtgggcag
     9721 caccgatgcg cgggagatcc aggtttcgcg ggcggg
//
LOCUS       DAOQJC010000142         9678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_3720_length_9678_cov_9.544009, whole genome shotgun sequence.
ACCESSION   DAOQJC010000142 DAOQJC010000000
VERSION     DAOQJC010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3720_length_9678_cov_9.544009"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PLE39_16430"
     CDS             complement(<1..683)
                     /locus_tag="PLE39_16430"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE69972.1"
                     /translation="MKLSVIIPVYNEVESIKVILERVQAQNLAEEIIVVDDGSKDGTR
                     DVLATLDGKNGVHIILHEKNKGKGAAVRTGMTAAQGDILLIQDADLEYDPRDYPQLLQ
                     PITEGVADVVYGSRFLGRAHRVTMFWHQVANQMLTLMTNILYNTILTDMETGYKVFRR
                     EVLNGMKIRANSFDFEPEFTAKILKRKFRIFEVPITFNPREYSQGKKIKLHDAFEAVW
                     TLIKYRFVE"
     gene            791..2125
                     /locus_tag="PLE39_16435"
     CDS             791..2125
                     /locus_tag="PLE39_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE69973.1"
                     /translation="MTRQPQQNPAPVGGFSPPQINNLEDTGLSTLWLQDLVLKVLYFR
                     GYLTGFKIAEEITLPFAGVVDQLLAALKQEKFIEVRSSQGGLGEGAYTYGITGAGIIR
                     AREAMERSQYAGPAPVPFAVYNEAIRRQKSGRLTVTTRTMRQILSQMVISENTFQRLG
                     PALNSGSSIFMYGPPGNGKTSIARAFGNLVLSQSMYIPYALYLDGQVIKVYDAVSHTV
                     APDNESGNGSGGTTSNLRSNTRRDPRWIKIRRPFIVVGGELTLEGLDLVFDDTTKFYE
                     APFQVKANGGILLIDDFGRQQVRPRDLLNRWIVPLENRVDYLRLHTGRKVEVPFDVLI
                     VFSTNLPPKDLVDEAFLRRLRHKIEIGDPSFEEYREIFKRVAQDKRVEYNDQGLAYLL
                     QEWYIKRNRKLRASHPRDLCDQILDISSYLAVPATMSREMIDHAGKAYFVDI"
     gene            2158..2910
                     /locus_tag="PLE39_16440"
     CDS             2158..2910
                     /locus_tag="PLE39_16440"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNE69974.1"
                     /translation="MFEHYPSPIILAHRGDLAHAPENTLPSFQQALQKGADGVELDAK
                     LTADGHVIVIHDMTVDRTTNGKGRVASFKLEDIRKLDAGSWFNEKFRGTQVPKLEEVF
                     ELIGKDKLINIELTNYSTPRDGLVVKVCELIKRHNIHKQIIFSSFLPSNLKIAAQTLP
                     EIPRGLLAMPGLVGLWARSFGFMFGEYQALHPHISSASREQIQRVHRLNRRVHVWTAN
                     SPEEIVRLKDWGVDGLFTDDPETAVRALGRSR"
     gene            2999..5737
                     /locus_tag="PLE39_16445"
     CDS             2999..5737
                     /locus_tag="PLE39_16445"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNE69975.1"
                     /translation="MKITRILFLLTLLVTLVPVPVQAQTSTPPAAVSAILNSMTPEER
                     VGQLFLVTFTGTDTSVDSQINDLISRYHVGGVVLLAENDNFIAAPDTASGTHALIDGL
                     QQLEWQASINPSEDATAGQEDTDAAYIPLFVGIAQEGNGYPTDQILNGLTPLPSEMAI
                     GATWNTDFAMRVGAVRGQELSALGFNLYLGPSLDVLEVPAVSGGDLGIRVFGGDPYWV
                     GEMSRAYVTGLHTGSDNQLLVVAKHFPGVGGSDRLPEEEVSTVRKSLEQLKQIELYPF
                     FAVTGNAITPESTADGLLVSHIRYQGFQGNIRATTRPISFDPQALKQILALPQFSTWQ
                     TNGGLIISDDLGTQAVSEFYASGGGQFSARTAARDAFLAGNDMLYLGNITSSDFPDTF
                     TTTTRIIEFFAQKYREDPAFAQRVDESAARILAVKARLYGNFTLQNTLVPAFRLTNIG
                     TAAEVPFEVARNAATLISPELQDLAAVMPLPPQVDESMVFITDTANIKQCTDCLEQPT
                     LAVDALQQAVLQLYGPQSGNQTNNFRLASYSLQNLETLLNGEETPFIEDDLTRAGWVI
                     FSLTDAAQGQANLISRFLRERPDLLREKRVILFAFGAPYYFDTTTISKFTAYYALYSK
                     QPQFVDVAARLLFQELSPVGASPVSISAVGYELISVMAPNPNQIIPLFLDLKPVATGT
                     ESPLTPEPTPIPLFKIGDTIAIRTGVIQDRNGHPVPDGTVVQFSMKLTGEGAGILQQE
                     DAVTTQGVARAAFGLDKPGLLEISVVSEPAVISEVVQLDVSQSGAVAVTVVVPELTEA
                     IEPTPEATPVEVVDAYISKEGYPRFSAWLVAMLFMVFSIWVVYSTGIRIIDRHSALRW
                     SLGIALGGLLAYNYLAFGLFNSMSWLTVSGLSGVVLFVFIGELMGFVAGWVWSRRR"
     gene            5881..6891
                     /locus_tag="PLE39_16450"
     CDS             5881..6891
                     /locus_tag="PLE39_16450"
                     /inference="COORDINATES: protein motif:HMM:NF014074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69976.1"
                     /translation="MAAQSTKNKEKQPPILFDKTQAIIKQVNKMLGGTLVTYFNNPRG
                     SVCHDDVLALHELLEKIGHQPKIFLFLKSSGGNGQASLRLVNLLRQYCDEVIAVIPLE
                     CASAATMITLGANEIQMGPMAYLTPVDTSLTHSLSPIDRDNDRVSVSLDELTRVVRLW
                     QGQNADARENPYKELFQHVHPLVIGAVDRAESLSIMLCKELLAYHIKDESEAERIASS
                     LNAKYPSHGYPILFEEAKRIGLKVKRLDPEINILLLELNELYSEMGQRATTDFDDTHA
                     HGNEILNIWESTSVLVYYQQDKDWFYRTEERRWISLNDVSRWRRMIQVGNKVEKSILH
                     IS"
     gene            7005..7604
                     /locus_tag="PLE39_16455"
     CDS             7005..7604
                     /locus_tag="PLE39_16455"
                     /inference="COORDINATES: protein motif:HMM:NF040769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HNE69977.1"
                     /translation="MANQPQLKFNDPAPDATLLDANGKPIQLSSLWKDRVVVLAFTRH
                     FGCPQCKEMVDQLIDAHQALTEKGMLLAIISHASAESARQFCEQRAPSATCLADPDRS
                     AYHAYGLYRGTFWQTLLSPRIWLSNRKLARTKGYKPELPSPGQDAYQMSGTFIIGPDG
                     RIRLPYYYEDIADHLPVDLILHGIMGVDWKRPYDSKPVV"
     gene            7610..8770
                     /locus_tag="PLE39_16460"
     CDS             7610..8770
                     /locus_tag="PLE39_16460"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE69978.1"
                     /translation="MTSFFLATYLAIKEVFRNRGRFLLVALVIALITVLVLFIAALGE
                     GLSDANRQYVANLDAQLIVFLEKSDYSITASRLETNTAKAVRRVDGVADAGPIYTSNS
                     EIVSLPEPLKISMLGVEAGRPGMPSLLEGRYFRGGAAREAVMDMSVAQRTNLKIGDEI
                     EIRSTQGTEDQFYTLKIVGLVDKQAYFFQPTIFVPASTWELMRPQSESDLNSDTPFPN
                     IVAVKLTDPTQVEVMKARLEDQVSNIEVADIETTINNIPGYTAQQGTVQTQGAFTLLI
                     GILVIGGFFQIQILQKVPQIGVLKAIGSSNFVVGLSAVIQIIIVTAMGVGIGAGLNYL
                     FSLGLPPNIPLAFNGTRSLVAIVLLLFIGPLGGMVSIIYAVRIEPLKALRLG"
     gene            8778..9509
                     /locus_tag="PLE39_16465"
     CDS             8778..9509
                     /locus_tag="PLE39_16465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE69979.1"
                     /translation="MSNIALEVRDLVKSFTLDGATINAVDHVSFQVRSGEFVALVGPS
                     GSGKTTLLSILAALLTPTSGQVLIDGQDLAQMNEKKRVKLRREKIGFTFQSNNLIPFL
                     TAQENVEFMLRLNGSLGRAGKVRSGELLARLGLAERLHNLPAQMSGGQQQRVAIARAL
                     IHQPAVVLADEPTASLDTERAFQVVETFAHLIHENERAGIMVTHDLRMCQYVDRVLQM
                     QDGKLVREYSTKEEIMELALGTVKH"
BASE COUNT         2233 a         2566 c         2446 g         2433 t
ORIGIN      
        1 tattctacaa acctgtactt gatcagggtc cacacggctt caaaggcatc atgtaatttg
       61 atcttcttgc cctgcgaata ctcacgaggg ttgaaggtga tcggcacttc aaagatccta
      121 aatttgcgtt tcaagatctt ggcggtgaat tcaggctcaa agtcaaaact gtttgcgcga
      181 attttcatgc cattcaacac ttcacggcga aaaaccttgt acccggtttc catatccgtg
      241 aggatggtgt tgtagagaat attcgtcatc agcgtcaaca tttggttggc aacctgatgc
      301 cagaacattg tgacacggtg ggcgcggcct aaaaagcgcg acccatagac cacatccgca
      361 acgccttcgg tgatgggttg aagcaactgc gggtagtcac gtgggtcata ttcgaggtcg
      421 gcatcctgaa tcaggaggat atcgccttgg gcggcagtca ttcctgtgcg gacagctgct
      481 cccttgcctt tatttttttc atgcaggatg atgtgtacgc cgttctttcc atccaatgtg
      541 gctaaaacat cgcgcgttcc gtctttggaa ccatcatcca caacaatgat ctcttcggca
      601 aggttctggg cttgcacacg ttctagaatg accttaatgc tttcgacttc attgtaaaca
      661 gggattatga cagatagttt catatgaact ggattataaa cgaaaagaag tataatcagc
      721 tggaaagaca aattttttcc accgctggga ttgcaagaaa aatttttatc aaagtctgga
      781 ttgcgccatt atgacaagac aaccgcaaca aaaccccgcg cccgttggcg gattttcccc
      841 gccacagatc aacaaccttg aggatacagg tctttcaacc ctttggcttc aggaccttgt
      901 cctgaaggtt ttgtatttcc gagggtatct gactggtttc aagatcgcag aagagatcac
      961 gctgcccttc gcaggcgtgg tggatcagct tctggccgcc ctgaaacagg aaaaattcat
     1021 cgaagtgcgg tcatctcaag gcggcttggg tgaaggtgca tatacctatg gcattaccgg
     1081 ggcgggcatc atccgcgctc gtgaagccat ggaacgtagt caatacgccg ggcctgcccc
     1141 cgtccccttt gcggtgtata acgaagccat tcgccgccaa aagagcggcc gcctcaccgt
     1201 gaccacccgc accatgcgcc agatcctttc gcagatggtg atctcagaaa acaccttcca
     1261 gaggctcgga cccgccctca actcgggttc atccattttt atgtacggcc cgcctggaaa
     1321 tggtaaaacc agcattgccc gtgcctttgg caaccttgtg ctcagccaaa gcatgtacat
     1381 tccctatgct ctgtacctcg atgggcaggt tatcaaagtg tacgatgccg tcagccatac
     1441 tgtggcaccc gataacgaat cagggaatgg cagcggcgga accaccagca acctgcgctc
     1501 caacacccgc cgcgacccgc gctggatcaa aattcgcaga ccgtttatcg tagtcggtgg
     1561 tgaattgact ctcgaaggtc tcgaccttgt gtttgacgac accacaaaat tctacgaagc
     1621 gccatttcag gtaaaagcca acggcggcat tttgctcatt gacgactttg gccgtcagca
     1681 ggtccgtccg cgtgacctgc tcaaccgttg gatcgttccg ctcgaaaacc gcgttgatta
     1741 cctccgcctg cacaccggcc gcaaagtgga agtccccttc gatgtgctca tcgtattctc
     1801 caccaacctg cctccgaaag accttgtgga tgaagccttc ctccgccgtc tgcgtcacaa
     1861 aatcgagatc ggcgatccct cctttgagga ataccgcgag atcttcaaac gggttgccca
     1921 ggataaacgc gttgaataca acgaccaggg attggcatat ctgctgcagg aatggtacat
     1981 caaacgcaac cgtaaactgc gcgcctccca cccgcgtgac ctttgcgatc agatcctcga
     2041 tatttcatcc tatcttgccg tacccgccac catgtcgcgt gaaatgatcg accacgccgg
     2101 caaagcctat tttgtggata tctaaccgca tcacccctga ccctgaacct gattccgatg
     2161 tttgaacatt atcccagccc aatcattctc gcgcatcgcg gcgaccttgc ccacgcaccg
     2221 gaaaacaccc ttccatcctt ccaacaggcg ctgcaaaaag gcgccgacgg tgtggaactg
     2281 gatgccaaac tcaccgccga tggccacgtg atcgtcatcc atgacatgac cgtggaccgc
     2341 accacaaacg gcaaaggtcg agtggcctcg ttcaaactgg aagacatccg caaactggat
     2401 gcaggcagtt ggtttaacga gaaattccgc ggcacccagg tgcccaagct cgaagaggtc
     2461 tttgaactca tcggcaagga taaactcatc aacatcgagt tgaccaacta ctccaccccg
     2521 cgtgatgggc ttgtcgtaaa agtatgcgaa ctgatcaaac ggcacaacat tcacaagcag
     2581 atcatttttt catcttttct cccgtccaat ttaaagatcg ctgctcaaac cctgcctgaa
     2641 attccacgcg gcttgttggc catgccgggg ctggttggtt tatgggcgcg ttcctttggg
     2701 tttatgtttg gggaatatca agccctgcat ccgcacattt ccagcgcgag tcgtgagcaa
     2761 attcagcgtg tgcatcgtct caaccgccgt gtccatgttt ggacggcaaa ctcccccgaa
     2821 gagatcgttc gcctcaaaga ctggggcgtg gatggcctgt ttaccgacga cccggaaaca
     2881 gccgtgcgag cacttgggag gagcaggtga gtttgtgcaa aaaacgattt cacaagacgc
     2941 cgcccttaaa gcggcgtcag ttttatttta aaagcaagtt gtgttataag gggatgcgat
     3001 gaaaataacc cgtattttgt ttttattgac cctgctggtc acgcttgtgc ctgttcccgt
     3061 acaagctcaa acctccacac ctccggcggc ggtctctgcc attttgaatt ccatgacccc
     3121 cgaagagcgc gttggtcagc tttttttggt tacgtttact ggtacggata ccagcgtcga
     3181 ctcgcagatc aatgacctga tctcccgtta ccatgtgggt ggtgtggtct tgcttgccga
     3241 aaatgataat tttattgccg ctcccgatac cgcctctgga acacatgcgt tgattgacgg
     3301 tctgcaacaa ttggaatggc aggcctccat caatccttca gaagatgcca ctgccggtca
     3361 ggaagatacc gatgccgcct atattccgct ttttgttggc atcgcccagg aggggaatgg
     3421 atatcccacc gaccaaattc taaacggtct cacccctctc ccaagcgaaa tggccatcgg
     3481 ggccacctgg aacaccgatt ttgccatgcg tgtcggcgca gtgcgcggac aggaactttc
     3541 agcgcttggc tttaacctat accttggccc atctttggat gtgctcgaag tgccagccgt
     3601 atctggtggc gaccttggga ttcgtgtttt tggcggtgac ccctattggg tgggggagat
     3661 gagccgcgcc tatgtgacag gcctgcatac tggcagcgac aatcaattgc tggtcgttgc
     3721 aaagcatttc cccggtgtag gcggctccga tcgcctgcca gaagaggaag tatccacagt
     3781 tcgaaaatct cttgagcagt tgaaacagat cgagctttac cccttctttg cagttaccgg
     3841 caatgccatc acccccgaat ccactgcaga tgggttgctg gtttctcata ttcgttatca
     3901 aggatttcaa ggaaacatcc gcgccaccac gcgtcccatc agtttcgatc cgcaggcttt
     3961 gaagcagatc ctggccctgc cgcaattctc cacctggcag accaatggcg gcctgatcat
     4021 cagcgacgac ttgggcacgc aagctgtcag cgagttttat gcatctggtg gcgggcagtt
     4081 ttcggcgcgt accgccgcgc gggatgcctt cctcgccggg aacgatatgc tgtacctggg
     4141 caacatcacc tccagcgatt ttccagatac gttcaccacg accacacgca ttattgaatt
     4201 ctttgcacaa aaatatcgtg aagaccctgc ttttgcccaa cgcgtggatg aatcagctgc
     4261 gcgcattctg gccgtcaaag cgcggctgta tggaaatttc accctgcaaa atacccttgt
     4321 accggctttt cgtcttacca atatcggcac agcagctgaa gtgcctttcg aagtagcgcg
     4381 aaatgcggcc actctcatca gcccggagct gcaagatctg gcagcggtca tgccgcttcc
     4441 gcctcaggtc gatgaaagca tggtctttat taccgataca gccaatatca aacaatgcac
     4501 cgactgtttg gaacaaccaa ccctggcggt ggatgcgttg caacaggcgg tcttgcagct
     4561 ttacggccca cagagcggaa atcaaacgaa taattttcgc ctcgcttctt attcgctcca
     4621 aaatttggag accctgctca atggagaaga aactcccttc attgaagatg atcttacccg
     4681 tgcaggctgg gtcatttttt cactgactga cgccgcacaa ggtcaggcaa acctgatcag
     4741 ccgtttcttg cgcgaacgcc ccgatctgtt gcgtgaaaag cgggtcattc tttttgcatt
     4801 tggtgcaccg tattatttcg ataccaccac gatctcaaaa ttcaccgcct actacgcact
     4861 ttatagcaaa caaccgcaat tcgtagatgt ggctgcccgt ttattgtttc aggaattatc
     4921 gccagtcggc gcttcccccg tctcaatttc tgcggtcgga tatgaattga tctccgtcat
     4981 ggcgccgaat ccgaatcaga tcattccgct ctttctggac ctgaagcctg ttgccacagg
     5041 aacggaatca cctctcaccc ccgagccgac tccgattccg ctgttcaaaa ttggcgatac
     5101 catcgcgatc cgcacgggcg taattcagga tagaaatggg catcccgtcc ctgatggaac
     5161 ggtcgtgcaa ttctccatga aattgacagg ggagggtgct ggtattttgc agcaagagga
     5221 tgcggtcact acacaaggtg tggcccgcgc cgcatttggt ttggataaac ccggtttgtt
     5281 ggaaattagc gtggtcagtg aacctgctgt gatctcggaa gtggtgcaat tggatgtttc
     5341 gcagtctggg gccgtggcag tgacagtggt ggtaccggag ctgaccgaag ccattgagcc
     5401 gacaccggaa gctacccccg ttgaagtggt ggatgcctac atcagcaagg aaggctatcc
     5461 gcgtttttca gcctggttgg tggccatgct ttttatggta ttcagcatct gggtcgtgta
     5521 cagcactggg atccgcatca tagaccgcca ttctgcgttg cgctggtctt tgggtattgc
     5581 tttgggcggc ttgctggcct acaactacct tgcctttggc ctgttcaaca gcatgagctg
     5641 gctcacggtc agcgggcttt ccggtgtggt tttatttgta ttcattggag aattgatggg
     5701 gtttgtcgca ggttgggtgt ggtcgcggcg gagataaagt tgtgaagttg catggtttaa
     5761 gccgccgttt ttctggcggc ttttttgttg caccaaacca gtacatttgt agcgatttca
     5821 cttttcacaa agcatgttat gatgattttt aatcactttg cttcaggagg ctagttatcc
     5881 atggcagccc agtcaaccaa aaataaagaa aagcaaccac ccatcttgtt cgataaaact
     5941 caggcgatca ttaaacaggt aaataaaatg cttggcggca cgttggtaac ctatttcaat
     6001 aacccgcgcg gaagtgtgtg ccacgatgat gtccttgcct tgcatgaatt gctggaaaag
     6061 atcggccacc agccgaagat ttttcttttt ttgaaatcca gtggtggcaa tgggcaggct
     6121 tccctccgcc ttgtaaatct gttgcgccaa tattgtgatg aggtcatcgc agtgatcccc
     6181 ctcgagtgcg cctccgccgc caccatgatc acccttggcg cgaatgaaat tcaaatgggg
     6241 ccaatggcct acctcacccc tgtggatact tccctgactc actcactgtc gccgattgat
     6301 cgtgacaatg accgtgtcag cgtcagcctc gatgaattga ctcgcgttgt ccgcttgtgg
     6361 caggggcaaa atgcagatgc cagggagaat ccctacaagg aattattcca gcatgtgcat
     6421 ccattggtca ttggcgcagt ggatcgggca gagtcactct ccatcatgct gtgcaaggaa
     6481 cttttggcgt accatatcaa ggatgagtcc gaagcggagc gtatcgccag ttccctgaat
     6541 gcgaagtatc cgtcgcatgg ttatcccatt ttgtttgaag aagccaagcg cattgggttg
     6601 aaggtcaagc ggcttgaccc tgagatcaac atactcctgc ttgagcttaa tgaactgtac
     6661 agcgaaatgg gccagcgtgc cacaaccgat tttgatgata ctcacgccca tggcaacgag
     6721 attcttaaca tctgggaatc aaccagtgtg ctggtctatt accagcaaga taaagattgg
     6781 ttctaccgca ccgaagaacg ccgctggatc tcgctcaatg acgtcagccg ttggcggcga
     6841 atgattcagg tggggaataa ggtggaaaaa tccattttgc acatctcttg ataaaacctg
     6901 tgcaaaacct gtgcaatctg atagaatctg agagtctcag tatggcaaaa ttccgttgct
     6961 gaacaagcgc ggaatttttg caaatattga aaaccaagga ctctatggca aatcaacctc
     7021 aactaaagtt taacgaccct gcacctgacg caaccctgct cgatgcaaat ggcaagccca
     7081 tccagctttc ttccctttgg aaggataggg tggtggtgct tgcctttacc cgccatttcg
     7141 gatgtccgca atgcaaggaa atggtggacc aattgatcga tgcgcatcaa gccctgaccg
     7201 agaaagggat gctgctcgca atcatttctc acgcctctgc agaatctgcc aggcagttct
     7261 gtgagcagcg tgctcccagc gccacctgcc ttgccgaccc tgaccgaagt gcctaccatg
     7321 cttatggttt gtatcgcggc accttctggc agacattgct ttcgcctcgc atctggcttt
     7381 caaatcgcaa gttggcacga accaaaggtt ataaacctga attaccatcc cccggacagg
     7441 atgcctatca aatgtctggt acgttcatca ttggccccga tggacgtatt cgcctgccgt
     7501 actactatga agatattgct gatcacctgc ctgtggattt gatcctgcac ggcattatgg
     7561 gtgtggattg gaaaaggccc tatgacagca aacctgtggt gtaaatagaa tgaccagttt
     7621 ttttctcgct acctatcttg ctataaaaga agtgtttcgc aaccgtggcc gttttttgct
     7681 ggtggccctg gtgatcgcgt tgatcacggt tcttgtccta ttcattgccg cgttgggtga
     7741 agggttgtcc gacgccaatc ggcaatatgt ggccaacctt gatgcacagt tgatcgtctt
     7801 tctggagaaa tcagattaca gcatcacagc cagcaggttg gaaacgaaca ctgccaaagc
     7861 ggttcgccgc gtagacgggg tggcagatgc aggcccaatc tatacctcca actcagagat
     7921 cgtttcactg cccgagccgc tcaagatttc catgcttggc gtggaagcag gccgccccgg
     7981 aatgccctca cttttggagg ggagatattt ccgcggtggc gccgcccgtg aagcagtgat
     8041 ggatatgagt gtggctcagc gcaccaattt gaagatcggt gatgagatcg agatccgttc
     8101 cacacagggc actgaagacc agttttacac attgaagatc gttgggctgg tggataagca
     8161 ggcctacttc ttccagccga ccatctttgt cccagcctct acatgggagt tgatgcgccc
     8221 acagtctgaa tcggatctga acagcgacac cccatttcca aacattgttg ccgtgaagtt
     8281 gaccgatcca acccaggtcg aagtgatgaa ggcgcgtctg gaagaccagg tgtcaaatat
     8341 cgaggtggct gatattgaga ccaccatcaa taacatccct gggtacaccg cccagcaagg
     8401 gacggtacag actcagggtg ccttcaccct gttgatcggc atcctcgtca tcggcggctt
     8461 tttccagatt cagatcctgc aaaaggttcc gcagattggc gttttgaagg cgatcggctc
     8521 ttccaatttt gtggtggggc tttcagcggt cattcagatc atcatcgtca ctgccatggg
     8581 ggttggcatt ggcgctggct tgaattacct gttctctttg gggttgccgc caaacattcc
     8641 attggccttt aatgggacac gctcgctcgt tgcgatcgtc ctgctcctgt tcattggccc
     8701 tcttggcggt atggtatcca tcatttatgc ggttcgtatc gagccgctta aggctcttcg
     8761 gttaggctag gtgcaaggtg tcgaatatag ctcttgaagt tcgtgatctg gtgaaatcct
     8821 ttacgcttga tggcgcaacc atcaatgcag tggatcatgt ttcatttcag gtgaggtctg
     8881 gtgaattcgt tgcgttggtc ggccccagtg gttcaggtaa gaccacattg ctttccattc
     8941 tggcggcttt gcttaccccc accagtggac aggttttgat cgacgggcag gaccttgcgc
     9001 agatgaacga gaaaaaacgg gtgaaattgc gccgcgaaaa gatcggcttc acctttcaat
     9061 ccaacaattt gattcctttc ctgaccgcac aggaaaatgt ggaattcatg ctgcgcctga
     9121 atggttcgct gggtcgtgcg gggaaggtcc gctccgggga attgctggca agactcgggt
     9181 tggcagaacg tttgcataac cttcccgctc agatgtcagg cggtcagcag caacgtgtgg
     9241 cgatcgcacg tgccttgatc catcaacccg ccgtggtgct ggccgatgaa cccactgcat
     9301 cattggatac cgaacgtgct tttcaggtgg tggaaacttt tgcccatctc atccacgaaa
     9361 acgaacgtgc gggcatcatg gtgactcacg acctgcgcat gtgccagtac gtggaccgtg
     9421 ttctgcaaat gcaggatggc aaactggtgc gcgagtattc tacgaaagaa gagattatgg
     9481 aactggcgct tggaactgtg aagcattaaa tttgtgccag ccattcatga aaaagagaca
     9541 gtcatttttt gaagtgactg tctctttttc gtttgttgat ttatggcaat ggaatgtttt
     9601 ttgccttcac aatgaggtac gcaccccagg ctcgatcaag caattcagag gggcagaaca
     9661 acggtttgcc tgttccac
//
LOCUS       DAOQJC010000143         9580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_3776_length_9580_cov_9.351391, whole genome shotgun sequence.
ACCESSION   DAOQJC010000143 DAOQJC010000000
VERSION     DAOQJC010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9580
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3776_length_9580_cov_9.351391"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PLE39_16470"
     CDS             complement(<1..1120)
                     /locus_tag="PLE39_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69980.1"
                     /translation="MTGQYRHLGIFSDWVAAAQRQQPLYQNTPPGPRTRELVRETLGF
                     CHTNESPAEVRIEREWQKDGLIGQEVSWSVGFGPRTHAYVLKPANATRTLPGIVALHD
                     HSDFKFYGKEKIADGPEEPSPVLNEFRKTPYGGRAYVNALAREGFVVLVHDVFLWGSR
                     RFPYETMIEMMGEKTSSRLEPQADEIERYNTAAYFHEHWVSKYCNILGTSMAGVVAYE
                     DRIALNYLLSRTDVDSEQIACIGLSGGGNRAALLTATHDRLKAAVIVGLMTTYEALLD
                     HNMSHTWMLFPFGWARHGDWPDIAACRAPMPLLVQYDLQDYLFTETGMKDAHQRLQRH
                     YASVGNQGAYTGQFYPGTHKFDLEMQSAAFAWLKQQLFN"
     gene            complement(1136..2368)
                     /locus_tag="PLE39_16475"
     CDS             complement(1136..2368)
                     /locus_tag="PLE39_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69981.1"
                     /translation="MSNKESKAIICPSCGAPLEAKEEAKRVKCGYCGTLVNISQSAQS
                     AAPVTPPAQTESKTIVDPEDAELDAAEQDIKRSWQIIIAIVAAIFFVVLGVIRSSKPA
                     YRPVPTLRPNNTPAATSTPRPPSLAKHLLTFNLKDSAPENPKDASALVVNSQGNIMVS
                     DTDAGHIRIFDETGKLITTITTNKNAPVTGMAVSTSGEILLVHNLEIHRYNTEGQETG
                     VMKDQLFEEVFTASDGSLYTLSVDQTISHYKQNGELDFQIKDAFGQYASTTDSPTLTV
                     DEDGQMYLLGINSGWVLKFSADGTFLNKFNAADSLKVTSSAPAGDSAFFGLVTPTKTP
                     TPKNILLQSIAVDANSRVYVCTSEGISIFSTSNQYVGSFRPASGNCEKMAFDNASHLY
                     ILTTENRVGKFEILTSTQ"
     gene            complement(2508..4043)
                     /gene="hutH"
                     /locus_tag="PLE39_16480"
     CDS             complement(2508..4043)
                     /gene="hutH"
                     /locus_tag="PLE39_16480"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNE69982.1"
                     /translation="METIYLDGNSLTFEQVQAVAYGKPNAPRVEISDTAKKLVMRAAD
                     AVQTLLSRGTIAYGITTGFGAFKDRIIPPDQVEQLQRNILVSHAVGVGEPFDIPTTRA
                     IMLIRANTLSRGHSGIRLATLQLLVEMLNAGVHPLIPEKGSLGASGDLAPLAHMALPI
                     IGEGKVEYQGAVMAGAEAFKRAGLTPIKLAAKEGLALTNGTTIMTAMGVLQTLRAERY
                     SQTADIAGCLSLEALNGTDLAFDERIHNLRPFPRQIECARYLRGLLKDSQFTREHASL
                     NVQDAYTLRCIPQVHGAVRDAIAYARWVFEIELNAVTDNPLIFIDEKEEITVLSGGNF
                     HGEPLAIAMDYLAFSVAELGNISERRIMRLTDESSNIHTLPAFLTKEGGLNSGFMILQ
                     YTAAALATENKVFAHPASVDTIPTSANVEDHVSMGATAAIKLRSVLDNVEKILSIELM
                     SAAQGVDFRKQVIGLEKNLGVGTRGVYKLIRTHIPFIESDTILYEHMETMRQLVAEGR
                     VLA"
     gene            complement(4056..6206)
                     /locus_tag="PLE39_16485"
     CDS             complement(4056..6206)
                     /locus_tag="PLE39_16485"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HNE69983.1"
                     /translation="MKQLLQNIKNGNTTVEDIPIPTPREGQALIKVAASLVSAGTERM
                     VVEFAEKSYLGKARSRPDLVKQTLDKAKREGIMPTVNAVFNRLDQPMALGYSTAGTIV
                     ALGKNMQGFKVGQRVACAGGGYATHAEYNVVPRNLLTPLPKNVDFESAAFTTLGAIAL
                     HGLRLAEPQLGENVAVIGLGLLGLMTIQLAAAAGCNVLGIDLDPKRIKLASSLGLEAV
                     SRQNAESASAAHFATANRGFDSIIICADTPSNDPVELAGVIARDRAKVVATGAVGLNF
                     PRKIYYEKEISFINSRSYGPGRYDSNYEENGNDYPIGYIRWTEGRNFQAVVDLMASGK
                     LKVTPLISHRFDIIEGVKAYEVITGKRKEPFLGVVLKYPEEKMKEERKIVFPSIAHRS
                     SSVVKLGVLGAGLYANATLLPVLKDNKDYELIGIASSGGLHAQHSGKKFGFQYATSSE
                     DEIINDPKINTVAILTRHDTHADLAIKALKAGKHVFVEKPLAINSNQLSVVSKQLLKT
                     DHCLLTTGFNRRFSPLARSLKSQLESLQEPKHLHYRVNAGFIPANHWTQDEAIGGGRI
                     IGEACHFIDTLTFLVGALPVKVSAQALPNNGKYREDNVSMTFTFADGSIGVVDYLANG
                     DKSMPKERLEVFCGGMVAILDDYVSLNVVKDGKRKEEKGAQDKGWRGEMAAFAEAVQG
                     KGEAPIPYGQIIAVTKSTFAAVESIRSGNPVEIK"
     gene            6421..8247
                     /locus_tag="PLE39_16490"
     CDS             6421..8247
                     /locus_tag="PLE39_16490"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNE69984.1"
                     /translation="MELGGYANKVAWVDLSAGKVEFKPIAEDDARKYIGGRGLGVKYV
                     FDNGPTVDALSPDNILCFMNGPLTGSEANMSGRMAVVTKSPLTGTVTDSHHGGWSAAR
                     LRWAGYDGLVFKGKAAKPTYIYIHDGQFELLDASEVWGKGVHDTVKHFKEKYGEKDLT
                     VITIGQAGENQVKYACWVNENDRASGRGGTGAVGGSKNLKAIVIKAEKKIVKAANRDK
                     WKEAHTKALAEIMDERVVTSPRKGGLSVYGTNVLMNMTNVIGGLPTKNSQFTGWEHAE
                     KVSGEYVNDNILVDNPTCHACPVACKKEVEIKDGPYAGLRMESVEYEPAWSVGTNCGN
                     DDIRVVAKMIDCANDYGFDAIEVGHPLSVWMEASERGYTNGSGKLNWGDGAAMVAAAE
                     MIAYRKGFGDVLANGANAVAAHFGHPELAMTVKGQGVPAYDPRGIKGMGLGYATSNRG
                     ACHLRGYTPAAEVVGNVLGPSTVADPLAWKGKGEMNFIFQNVHAVTDCLDICKFATFA
                     ESLDSFAMQYEAMTGVPCDAGHLLKVGERVYNLERHINNLNGFREGSDTLPKRFTDEP
                     STYGGSKGQVCELDDMLVEYYEKRGWNNGVVPESKLKELEII"
     gene            complement(8397..8675)
                     /locus_tag="PLE39_16495"
     CDS             complement(8397..8675)
                     /locus_tag="PLE39_16495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNE69985.1"
                     /translation="MKVNFFATLRDIAGGKTVEFDVDHGITAQKLLDTIVEKYPLMKK
                     ELIHPDGHMYGHVHFFINGRDVQFMDEAFETKITAEDTVNVFPAVGGG"
     gene            complement(8692..8997)
                     /locus_tag="PLE39_16500"
     CDS             complement(8692..8997)
                     /locus_tag="PLE39_16500"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNE69986.1"
                     /translation="MQLRFYANMRTTVGMSSLEIGDEQIASFRGLLKVLLEKYPDTAF
                     HLLNTQGDLRPDVPVYVDGRNPRLTPLGIDTPLNPDSVVSFFSPISSGKINVEVLRD"
     gene            complement(9119..>9580)
                     /locus_tag="PLE39_16505"
     CDS             complement(9119..>9580)
                     /locus_tag="PLE39_16505"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNE69987.1"
                     /translation="KLSLPKMDFNEETQAALTEAFNSGCNVSFAGNNWGGANDVCNFV
                     ESGTMSIAWTGEVSPCWPLMHTHMSYLHGKPRLSKKHVIGDVRKQTLEEIWLNPDYVA
                     YRERLHNFVFAPCTFCGGCDLSETNEEDCLGNDIAPVCGGCLWAQGIVQCP"
BASE COUNT         2198 a         2524 c         2498 g         2360 t
ORIGIN      
        1 tattaaataa ctgctgtttc aaccacgcaa acgcagcgga ctgcatctcc aggtcaaatt
       61 tatgggtgcc ggggtaaaac tgtcccgtgt acgcgccttg gttgcccacg ctggcgtagt
      121 gacgttgcag acgttggtgg gcgtctttca tgccagtttc ggtgaagaga taatcttgca
      181 agtcatattg caccagcaaa ggcatgggag cgcggcaggc ggcgatgtcg ggccagtctc
      241 cgtggcgcgc ccagccgaag ggaaagagca tccacgtgtg cgacatgtta tggtcaagca
      301 gggcttcgta ggtggtcatc agccccacga tcacggcagc tttcaaacgg tcatgtgtgg
      361 ctgttaacag agcggcgcga ttcccgccgc ctgataaacc gatacaggcg atctgctcgc
      421 tgtccacatc cgttcgggag agcagataat tcaacgcgat gcggtcttca taagcgacca
      481 cacccgccat gctggtgccg aggatgttgc aatacttcga aacccaatgt tcatggaaat
      541 acgccgccgt gttgtagcgt tcgatctcgt ctgcctgcgg ctccaagcgt gacgaggtct
      601 tctcccccat catctcgatc atcgtctcat acggaaagcg gcggctgccc cacaaaaaca
      661 catcgtggac gaggacaacg aatccctcgc gcgccagggc gttgacgtat gcccttccgc
      721 cgtagggagt tttgcgaaac tcgttcaaca caggcgaagg ttcttctggt ccatccgcga
      781 tcttttcttt gccgtaaaac ttgaaatcgc tatgatcgtg caacgccaca atccccggaa
      841 gagtccgcgt ggcgttggcg ggtttcaaaa catatgcatg cgtccgcgga ccaaacccca
      901 ccgaccacga aacctcctgc ccgatcaacc catccttttg ccactcgcgc tcaatgcgga
      961 cttcggcagg agattcgttg gtgtggcaaa agcccagagt ttcacgcacc agttcccggg
     1021 tccgcgggcc cgggggcgtg ttttggtaaa ggggctgttg tcgctgtgcg gcggcgaccc
     1081 agtcactgaa aattcccagg tggcgatatt gtcccgtcat gtgatgtatt cccgtttatt
     1141 gtgtggaggt caggatttcg aatttcccca cccgattttc ggtagtgagg atgtacaaat
     1201 ggcttgcgtt gtcaaaagcc attttttcgc agttcccgga agcgggtctg aacgaaccga
     1261 catattggtt gctggtgctg aaaatggaga ttccctccga ggtgcaaaca taaacacggc
     1321 tgttggcatc cacggcaatg gattgcagaa ggatgttctt gggggtcggg gttttggtgg
     1381 gggtgactaa accaaaaaat gcagaatcac cggcaggggc agacgaagtg accttcaatg
     1441 agtcggcggc attgaatttg ttgaggaagg ttccatcggc tgaaaacttg agcacccatc
     1501 cgctgtttat tccaagcaga tacatctgtc cgtcctcatc cacggtcagg gtgggggagt
     1561 cggtggtgct ggcgtattga ccaaaggcat ccttgatctg aaaatccagt tccccgtttt
     1621 gtttgtagtg gctgatggtc tggtcaacag atagggtgta cagcgaccca tcagaagcgg
     1681 tgaagacctc ttcaaaaagt tggtctttca tcacgcctgt ttcctgacct tccgtgttgt
     1741 agcggtgaat ttccagattg tgtacaagca ggatctctcc acttgtggaa acggccatcc
     1801 cagtgacggg ggcatttttg ttggtggtta tggttgtgat gagttttccc gtttcatcaa
     1861 agatacgaat atggcctgca tcggtatcac tcaccatgat attgccctga ctgttcacaa
     1921 ccagagcgga ggcatctttg gggttttcgg gggcgctgtc tttcaagtta aaggtcaaca
     1981 ggtgtttcgc caggcttggc ggacggggtg tgcttgtagc ggctggggta ttattgggcc
     2041 gcagggttgg aacaggacga taggcaggct ttgaggaacg gatcaccccc aagacgacga
     2101 agaaaatagc cgcaacaatg gcgatgatga tttgccacga cctcttaata tcctgttcgg
     2161 cggcatcaag ttcagcatcc tctgggtcta ctatggtttt actttcggtt tgtgcaggcg
     2221 gtgtgacagg tgctgcagac tgggcgcttt gtgaaatatt caccagtgtg ccgcaatatc
     2281 cgcacttgac gcgtttggct tcttctttgg cttcgagcgg cgccccacag gatgggcaaa
     2341 tgatggcttt ggattctttg ttgctcatgt tctctctctt gggtgaaatt tttacctagg
     2401 ttaatgcaca ggttccacta cgtcgatagg aaatcgtggg gaattgagaa agcaaaaggc
     2461 cgaggcgcca acctcgacct tttgctttcg gttctgtgaa aaacgggtta cgccaaaacc
     2521 cttccttccg ccaccaattg acgcatcgtt tccatatgct catacaggat cgtgtcagat
     2581 tcaataaaag ggatgtgagt acggatcaat ttgtagacgc ctcttgtccc cacgcccaaa
     2641 tttttctcaa ggccgatgac ctgtttgcgg aagtccacgc cctgcgcggc agacatcaac
     2701 tcgatggaga ggattttctc cacgttatca agcacgcttc gcaacttaat tgcagcggtt
     2761 gctcccatac tgacatggtc ctccacgttt gcagaagtgg ggatcgtatc cacgctggca
     2821 gggtgtgcaa agaccttgtt ctcggttgcc aatgccgccg ctgtgtattg caagatcata
     2881 aagccagaat tcaatccgcc ttctttggtg aggaacgcag gcagggtgtg gatgttcgaa
     2941 ctttcatcgg tcaggcgcat gatgcggcgc tctgaaatat tgcccaactc cgccaccgaa
     3001 aaagccagat aatccatcgc aattgccagc ggttcaccgt ggaaatttcc gccagataaa
     3061 accgtgatct cctccttttc atcaatgaag atcagcggat tatccgtcac ggcattcagt
     3121 tcgatctcaa aaacccagcg tgcataggca atggcatcac gaaccgcccc atgcacctgt
     3181 ggaatgcagc gcaaggtgta cgcatcctgc acattcaacg aagcatgttc acgggtgaat
     3241 tggctgtctt tcagcaaacc ccgcaaataa cgcgcacact cgatctggcg cggaaaaggc
     3301 cgcaggttgt ggatccgctc atcgaaggcg aggtcggtac cgttcaatgc ttcgaggctt
     3361 aagcagcctg caatgtcagc ggtttggctg tagcgttctg cgcgcagtgt ttgcagcacg
     3421 cccatcgctg tcatgatggt cgtgccattc gtcagtgcca gaccttcttt cgcagccagt
     3481 ttgatcggcg tcagacccgc acgtttgaat gcctcagcgc cagccatcac tgcaccctga
     3541 tattccacct tcccctcgcc gatgatcggc aaagccatgt gcgccagcgg agcaaggtca
     3601 ccgcttgcgc cgagcgatcc cttttctggg atcagcggat gcacccctgc attgagcatc
     3661 tccaccagca attgcaaagt tgcgagtcga ataccggaat gtccgcgcga gagtgtgttg
     3721 gcgcgtatca gcatgatggc ccgcgtggtt gggatatcga acggttcgcc gacacccacg
     3781 gcatggctca ccaagatatt gcgctgcagt tgctccacct ggtcgggtgg aatgatgcgg
     3841 tctttgaacg ccccgaagcc tgtggtgatt ccatacgcga tcgtgccgcg tgaaagcagg
     3901 gtctgcacgg catcggcggc acgcatcacc agtttctttg ccgtatcgct gatctccact
     3961 cgcggcgcat ttggctttcc ataagccact gcctgcacct gttcaaatgt taaactgttt
     4021 ccatccagat agatcgtttc catgcaacct cgaggttatt ttatttcgac aggatttcca
     4081 cttcgaatcg attccaccgc cgcaaatgtg gatttggtca cagcgatgat ctgcccatag
     4141 ggaatcggcg cttcgccctt cccttgaact gcttcagcaa aagcggccat ttctccccgc
     4201 cagcctttgt cttgtgcccc tttttcttcc ttcctttttc cgtctttcac cacattcaat
     4261 gagacataat catccagaat cgccaccatg cccccgcaaa agacttcaag ccgctccttc
     4321 ggcatggatt tatcgccatt ggcaaggtaa tccaccacgc cgatggaacc atccgcaaaa
     4381 gtgaaggtca tcgagacatt gtcctcgcgg tatttgccgt tgttgggcaa agcctgcgcc
     4441 gaaactttca caggcaaagc accgaccaaa aaagtgagcg tgtcaataaa atgacaggct
     4501 tcaccgatga tgcgtccgcc gccgatggct tcgtcctgag tccaatggtt ggcagggata
     4561 aaaccagcgt tgacgcggta gtgcaggtgt ttgggttcct gaaggctttc aagttgagat
     4621 ttgagcgacc gggcaagggg ggaaaatctt ctattaaacc cagtcgtcag taagcagtga
     4681 tcagttttca gcaactgttt actgacaact gacaactgat tactatttat cgccaacggc
     4741 ttttccacaa acacatgctt cccagccttc aaagccttta tggcgagatc ggcatgggtg
     4801 tcgtgtctgg tgaggatggc aacggtgttg atttttgggt cgttgatgat ctcgtcttct
     4861 gaagatgtgg catattggaa accaaacttt ttgcctgagt gctgggcatg caaaccacct
     4921 gaagaggcaa tgccgatcag ttcataatct ttattgtctt ttagcactgg cagcagggtg
     4981 gcgttggcat aaagccctgc gccaagaaca cccagcttga ccacagacga tgaacgatgg
     5041 gcgatggaag gaaatacaat ttttctttcc tccttcatct tttcttcagg gtatttcaac
     5101 accaccccaa ggaaaggctc ttttctcttt cctgtaatga cttcgtacgc tttgacccct
     5161 tcgataatat cgaagcgatg gctgatgagc ggtgtcactt tcaactttcc gcttgccatc
     5221 agatccacta ctgcctgaaa atttcgtcct tccgtccagc ggatgtagcc gatggggtag
     5281 tcattgccgt tctcttcgta attcgagtcg tagcggcccg ggccgtagga gcgggagtta
     5341 atgaaggaga tttctttttc gtagtagatc tttctgggga aattcaggcc aactgctccc
     5401 gtggctacta ctttggcacg gtctcgagcg atgactcccg ccagttccac aggatcgttg
     5461 gagggagtgt cggcgcagat gatgatgctg tcgaatccac ggttggcagt cgcgaagtgt
     5521 gccgcagatg ctgattctgc attttggcgg gagacagctt ccagtccgag tgaagaggcg
     5581 agtttgattc gctttgggtc aaggtcaatg ccaaggacgt tgcatcctgc tgcggccgcc
     5641 agttgaatcg tcattaatcc caaaagaccc aggccgatga cagcgacatt ttctccgagt
     5701 tgcggctctg ccagtcggag gccgtgcaag gcaatggcac caagggtggt gaaggcagcg
     5761 gattcaaaat cgacattttt ggggagtggc gtgagcaaat tgcgcgggac aacattgtat
     5821 tcggcatggg tggcataccc gccgcccgca cacgcaaccc gctgaccgac tttgaagccc
     5881 tgcatatttt tgccgagtgc cacaatggtt ccagcagtgg agtagccaag tgccatcggt
     5941 tgatcgaggc gattgaacac cgcgttgacc gtgggcatga taccttcgcg tttggcttta
     6001 tcgagggtct gcttcacgag gtcaggacga gagcgtgcct tgccgagata gctcttttcc
     6061 gcaaattcca ccaccatgcg ttcggttcct gctgaaacaa ggcttgctgc caccttgatc
     6121 agagcctgcc cttcacgtgg ggtggggatg gggatatctt ctactgtggt atttccattt
     6181 ttgatgtttt gaaggagttg tttcatggat gagctttcta tagttttacc aagtattgaa
     6241 agtataacgc aattcaaaat gcgtggactt gatgtgaaaa ataacgcaaa ggaagaatgt
     6301 cacctttacc gttaagtttt tacttaatta ttggaatcga tttcaccaaa tgctattctg
     6361 aacgcaggac ggacggcttg cgccgttcta tattcatttg tcaaaaaaag gagattcatt
     6421 atggaactcg gtggttatgc aaacaaggtt gcttgggtag acctttccgc tggtaaagtg
     6481 gaattcaaac ccatcgccga agacgatgcc cgcaaataca tcggcggacg cggtttgggc
     6541 gtgaagtatg ttttcgacaa cggccccacg gtcgatgccc tctcccccga taatatcctc
     6601 tgcttcatga acggcccgct cacaggctct gaagccaaca tgagtggacg catggctgtt
     6661 gtgaccaaat cgcctctcac tggaaccgta actgattctc accatggtgg ctggtctgct
     6721 gcccgtttac gctgggctgg atatgatggc ttggtcttca aaggcaaggc tgccaaaccc
     6781 acctatattt atattcatga tggccagttc gaactgcttg atgcctctga agtttggggt
     6841 aagggcgttc acgacaccgt gaagcacttc aaagaaaaat atggtgaaaa agacctcacc
     6901 gtcattacca ttggtcaggc tggtgaaaac caggtcaagt atgcctgctg ggttaatgaa
     6961 aatgaccgcg cttccggacg cggcggcacc ggcgcagttg gtggctcaaa gaacctcaaa
     7021 gccattgtca tcaaggctga gaagaagatc gtcaaagccg cgaatcgcga taagtggaaa
     7081 gaagcccaca ccaaagccct cgccgaaatc atggacgagc gcgttgttac ctctccccgc
     7141 aagggtggtc tctctgttta tggcaccaac gtgctcatga acatgaccaa cgtcatcggc
     7201 ggtctgccca ccaagaacag ccagttcacc ggttgggaac acgctgaaaa agtcagcggc
     7261 gaatacgtca acgacaacat cctcgtggat aaccccacct gtcacgcctg ccccgttgca
     7321 tgcaagaagg aagttgaaat taaagatggt ccgtacgctg gtctccgcat ggaatcagtg
     7381 gaatacgaac ccgcctggtc ggtcggcaca aactgcggca atgacgatat ccgcgttgtc
     7441 gccaagatga tcgactgcgc caacgactat ggcttcgatg ccatcgaagt tggtcacccg
     7501 ctctccgttt ggatggaagc cagcgaacgc ggctacacca acggctctgg caaactcaac
     7561 tggggcgacg gcgccgccat ggttgctgcc gctgaaatga tcgcctaccg caagggtttt
     7621 ggcgatgttc tcgccaacgg cgcgaatgca gttgctgctc actttggcca ccccgaactc
     7681 gccatgaccg ttaagggtca gggcgtgccc gcatacgatc cgcgcggcat taagggcatg
     7741 ggcctgggct acgccacatc caaccgcggt gcctgccacc tgcgcggcta cacccccgcc
     7801 gccgaagtcg tcggcaacgt acttggacct tcaaccgttg ctgatccgct cgcgtggaag
     7861 ggcaagggcg aaatgaactt catcttccag aacgttcacg ccgtcaccga ctgcctcgac
     7921 atctgcaaat tcgccacctt cgccgaatcc ctcgactcct tcgccatgca atacgaagcc
     7981 atgacgggcg tcccctgtga tgcgggtcac ctgctcaagg tcggcgaacg cgtttacaac
     8041 ctcgaacgcc acatcaacaa cctcaacggc ttccgcgaag gctctgacac cctgccgaag
     8101 cgattcaccg atgaaccctc cacctacggt ggcagcaagg gtcaggtctg cgaactcgac
     8161 gacatgctcg tggaatacta cgagaagcgc ggatggaaca acggcgttgt gcccgaatcc
     8221 aaactcaagg aattggagat catctaattc ccgcttcatt cctacctgaa gcctgattct
     8281 gttttctgaa acagaatctc cccgtaaaca aaaagcaggg cgactcacgt cgccctgctt
     8341 cgtttttctg tactgcaaaa tttattttgc aattacagga cgaaattcat ttcgtcctac
     8401 ccaccgccaa ccgcagggaa gacattcacg gtatcttcag cggttatttt tgtttcaaaa
     8461 gcctcatcca tgaactgcac atcgcgcccg ttgataaaaa agtggacatg cccatacata
     8521 tgcccgtctg gatggatcaa ttcctttttc atgagcgggt atttttcgac aatcgtatcc
     8581 aataattttt gggcggtaat gccgtggtcc acatcgaatt ccacggtttt tccgccagca
     8641 atatcacgca gggtggcaaa gaaattcact ttcatgagat catctccttt attaatcgcg
     8701 tagaacttcc acgtttatct taccactgga aatgggagaa aaaaagctga cgacagaatc
     8761 aggattgagg ggagtatcaa ttccgagcgg agtcagacgc gggtttcttc catccacata
     8821 gacaggcaca tcaggccgca agtcaccttg agtgttgagc aggtgaaacg ctgtatcggg
     8881 gtatttttcc agcaatacct tcaatagccc gcgaaatgaa gcaatctgct catctccgat
     8941 ctcaagcgaa gacattccaa cggtcgtgcg catattggcg taaaaacgta gctgcatttt
     9001 tgcttccttt ttcaaatttt atcatctttg tttttaggat gttaacgtat gttcatgata
     9061 aaaaatcacc ctgaattttt tcagggtgac atggttttga tttatgaaga agcttgtttc
     9121 atgggcactg cacaattccc tgcgcccaca aacacccacc acagacaggg gcgatgtcat
     9181 tgccgaggca gtcctcttcg ttggtctcgg aaaggtcgca gccgccgcag aaggtacaag
     9241 gcgcaaagac aaagttatgc aggcgttcac gataggctac ataatcgggg tttagccaga
     9301 tctcttcgag ggtttgtttg cgcacgtcac cgatgacatg ttttttagag aggcgcggtt
     9361 tgccatgcag gtagctcatg tgggtgtgca tcaatggcca gcaggggctg acctcacctg
     9421 tccacgcaat ggacatggtt ccgctttcaa caaaattaca gacatcgttc gcgccgcccc
     9481 aattgttgcc tgcaaaactg acattgcagc cactgttgaa ggcttcagta agagccgcct
     9541 gggtttcttc gttgaagtcc attttgggca ggctgagttt
//
LOCUS       DAOQJC010000144         9390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_3878_length_9390_cov_10.259454, whole genome shotgun sequence.
ACCESSION   DAOQJC010000144 DAOQJC010000000
VERSION     DAOQJC010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9390
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3878_length_9390_cov_10.259454"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PLE39_16510"
     CDS             complement(<1..776)
                     /locus_tag="PLE39_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNE69988.1"
                     /translation="MEIKFGTHTIGLNHPTYFIADIAANHDGDMERAKKLIRLAKEAG
                     ADAAKFQNFDAPKIVSDYGFKAMSGGQVSHQASWKKSVFEVYKGASIPFEWSMTLVEE
                     CKEVGIDYFSSPYDFEAIDFLDQYVEVYKAGSGEIDWIEALERMAGKGKPFFIATGAS
                     TIGEVQKAVHAILKLNKQLVLMQCNTNYTANPNNYDHLHLNVLKTYAAMFPDVILGLS
                     DHTHSVAPVLGAVTLGARVIERHFTDSNDREGPDHKFAMNP"
     gene            complement(814..1380)
                     /locus_tag="PLE39_16515"
     CDS             complement(814..1380)
                     /locus_tag="PLE39_16515"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNE69989.1"
                     /translation="MTDLNPKQNPDQHLTPQQLAREVVYQSRWVNLYKDTVKFPNGFV
                     IDKFHLLDFEHEAVTAIMENTAGEVIFVLIPRYATGTVEWELPAGGMEIGETAIEAAR
                     RELLEETGYSSEGHVLVYSYYPMNGNANQVFHIVHCKVTELVQGFDPNEVSDVHWFTR
                     KEIKQMIKEKKITDGFTLTALLLWLSED"
     gene            complement(1694..2605)
                     /locus_tag="PLE39_16520"
     CDS             complement(1694..2605)
                     /locus_tag="PLE39_16520"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE69990.1"
                     /translation="MKLLITGASGLLGINLAQETMHTHEIVGVDRGKLAHAPFPILKT
                     DLLAPGAVDSIIETARPDWVINCAALANLEECEENPDLARRLNTDMPAQLAKACKLRG
                     TAFIHISTDAVFDGTSLAFYKEEDEPNPLSLYAKTKLEGERAVLAEDPNAIVTRVNFY
                     GWSLTGRRSLAEFFFHNLTYNKSMSGFTDVKFCPMLVNHTARTLVKMLARGLHGLYHL
                     VGPQAMSKYQFGVEIARKFGLREGEISPKSINTSSLNARRSHNLCLSVNKISTDLGEA
                     LPEFSTGLNEFYTQYQQDYPQKIRSYQ"
     assembly_gap    2631..2730
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2845..3855)
                     /gene="pseB"
                     /locus_tag="PLE39_16525"
     CDS             complement(2845..3855)
                     /gene="pseB"
                     /locus_tag="PLE39_16525"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HNE69991.1"
                     /translation="MDWKNQVVLVTGGTGSFGKKFTKILLEEKQPKKVIIFSRDELKQ
                     HEMQVGGFNDPRLRYFIGDVRDRERLVRAMHGVDIVVHAAALKQVPACEYNPMEAIKT
                     NIMGTANVVEAALDAGVKKVLTVSTDKAVSPANLYGATKLAAEKLTIQSNAYAGGSAT
                     RFSCVRYGNVVGSRGSVVPLFLKQRSGGKITITDDRMTRFWLSLEQGVRFVIDCIEQM
                     EGGEVFVPKIPSTKVVDLARAIAPDAEINIIGIRPGEKLHEMLISEDEARHTIELEKM
                     YVVQPAEATWFGYSWQDKGKSLNEGTYYSSDNNSEWLDIEGIKKYIAPFEELFAQGKL
                     EG"
     gene            complement(3940..4737)
                     /locus_tag="PLE39_16530"
     CDS             complement(3940..4737)
                     /locus_tag="PLE39_16530"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE69992.1"
                     /translation="MQRFTDQQYLTTDQYKDSANLDARIAIHQRFSTNPQGWFNWVFD
                     ALVTLPADAHVLELGCGAGTMWNQCADRIPAGWTITLSDLSDGMLDSAWRNLVVTGRS
                     FKFEKIDAQSIPYADGTFDAVIANHMLYHVPDRKKALTEIRRVLKSDGCLIASTVGAN
                     HMKEMYGWLQRVNKNPRPDMFTNPFTLESGLAQLLEVFPNVEKSQYVDNLRVTELDPL
                     MNYIRSSIPAEDISEPEFETLKKEFAAVLKKDGEIFISKDSGLFKAL"
     gene            complement(4739..5560)
                     /locus_tag="PLE39_16535"
     CDS             complement(4739..5560)
                     /locus_tag="PLE39_16535"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE69993.1"
                     /translation="MTNETKQKVREFYDEIGWMQEDDGNYQNARYEDLRPVSREYIHK
                     TRLRVMNGLIPTGRFMLDAGSGPVQYEEYKTYSAGYEKRVCLDISIQALREARTRIGD
                     HGLFVVGDLANLPFKKDAFDGAVSMHAIHHLALPEHPRAYAEIHRVLAPGRTAAIVNG
                     WSDPLISRMAEPFIGLLRKVTGRDAKKKKEWLSEDAPAGTFVEKMSPAWLKREIGSKM
                     TIELKPWRGLSTRILRNFVRPFGGRAFLKFIFWLEGIFPKFFAENGQYPLIVVKK"
     gene            complement(5630..7264)
                     /locus_tag="PLE39_16540"
     CDS             complement(5630..7264)
                     /locus_tag="PLE39_16540"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69994.1"
                     /translation="MTNTLRNLLGDLPFTAEIDWMLRSKNRPRKDHYNLGRLEKSLPA
                     AVEVVKPFAEKAPRGKKVLFFATLHYWIEQSAYLGLVLAGLGHDVTLLTLPYSEWHKE
                     KDKFTQRQRVLHTHDSLAVLSPLVKHVSMLDMQSSARVSHGTLRAVKHDDLPEKILAD
                     IKEVSLWDVQYTLMREEVDMNDAGDRALYDLRIERNTFAAKAALQFMQTEKPDVVLIP
                     NGLILEMGIVFRVARHLGIDAVTYEFNDQREQIWLAQNSSIMQQETDYLVDARCSLPM
                     TDAMFERVADLENARRGARVWGKSKRLWQYVSSQGAAETRKMLNLDDRPVVMLAANVL
                     GDSLTLGRDIFASSMTEWITKTVQYFARRTDVQLVIRVHPGEKLVPQAKSMGTVVKDA
                     LPELPSHIHVIGALDKVNTYDLIEIADVGLAYTTTVGLETTMNGRPVISCGRTHYRGR
                     GITIDPNTWEEYYATLEKVLSDIPAHQMTEKQIEFAWNYAYRFFFEYPRPFPWRLMNF
                     WDDLAEHPLESVLSEEGKAQFKDTFDFLVGEPFSWK"
     gene            complement(7330..8619)
                     /locus_tag="PLE39_16545"
     CDS             complement(7330..8619)
                     /locus_tag="PLE39_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE69995.1"
                     /translation="MTTAISVKNIGKQYKIGAAETKFRYNMLRDVIVDTVSAPIRLAK
                     AMIGRSDRRLNQNFVWALKDVSFDLEEGKVLGIVGRNGAGKSTLLKILSRVTEPTTGT
                     VSVRGRVGSLLEVGTGFHPELTGRENIYMNGAILGMRRTEIDSKFDEIVDFSEVSQFI
                     DTPVKRYSSGMYLRLAFAVAAHLEPEILVVDEVLAVGDAEFQKKCLGKMGDVAQQGRT
                     VLFVSHNMSAILRLTQEAIVLNKGQLIMRGPTQEAVDYYLSSGQSQAGERTWGAEDVP
                     AAAMPFTPISLKVKERSGKVVDTIRSTEPVTVEFEYKLSAPVTGLRVGLYVSTMRGEY
                     VFTSFDTDSPEFYENFDSRNAGHYISRAELPADIFNEGRYTVGVNASSFGVRRYFMDE
                     NALAFNVDISGAPGTQWAEPRVGPVRPRLNWKIEKIG"
     gene            complement(8654..9016)
                     /locus_tag="PLE39_16550"
     CDS             complement(8654..9016)
                     /locus_tag="PLE39_16550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNE69996.1"
                     /translation="MAMIQKFEDIQAWQEARTLVKMVYQLTNKDKFSKDFGMRDQIRR
                     ASVSVMNNIAEGFDCESKIEFSRFLGIARRSAVEVQSMLYAALDVEYIDQNEFDIHYE
                     QARKTKALVGGFKRSLTK"
     gene            complement(9070..>9390)
                     /locus_tag="PLE39_16555"
     CDS             complement(9070..>9390)
                     /locus_tag="PLE39_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE69997.1"
                     /translation="ALGVALWLSAINVQYRDVNQALPFLTQFWLFATPVAYSASVISE
                     KWQVVYSLNPMAGVVNGFRWALLGTGNGPDLSLWVSVGISVLTFISGLFYFRSMEKTF
                     ADTI"
BASE COUNT         2100 a         2384 c         2477 g         2329 t
ORIGIN      
        1 gggttcatcg caaacttgtg gtcggggcct tcgcggtcgt tgctgtcggt aaagtggcgt
       61 tcgatgacgc gcgcgcccaa cgtcaccgca ccgagaacag gggcgacaga atgggtgtgg
      121 tcagagagtc ccagaatcac atcggggaac atggctgcat aggttttcag cacgttcaag
      181 tgcaaatggt cgtaattgtt cgggttggcg gtgtaattgg tgttgcactg catcagcacg
      241 agttgtttgt tcaatttcag aatcgcatgg acagcctttt gaacttcgcc aatggtggat
      301 gctcccgtgg caatgaagaa gggcttgcct ttcccagcca tgcgttcgag cgcttcgatc
      361 cagtctattt caccggagcc agccttgtac acttccacat actgatcgag gaaatcaatg
      421 gcttcgaaat cgtagggcga agagaagtag tcaatgccga cttctttgca ttcttcaacc
      481 agagtcatcg accattcaaa tggaatggac gcgcctttgt acacttcaaa aacggatttc
      541 ttccacgaag cctgatgact gacctgtccg ccgctcatgg ctttgaaacc gtaatctgaa
      601 acgatcttgg gcgcgtcgaa attctggaat ttggcggcat cggcgcctgc ttccttggcg
      661 aggcggatta gttttttggc acgctccatg tcaccgtcgt gattggcggc aatatctgca
      721 atgaagtagg tgggatggtt caagccgatg gtgtgggttc caaatttgat ttccattttt
      781 tgttcctccg ggtggatggt ttttcttttt atttcagtcc tcgctcagcc agagcaataa
      841 cgcagtgagg gtaaatccgt ctgtaatctt cttttccttt atcatttgct tgatctcttt
      901 ccgcgtaaac cagtggacat cactgacttc gttcgggtcg aaaccctgca ccagttccgt
      961 aaccttgcaa tgcacaatgt gaaagacctg attggcattt ccattcatcg ggtaatagga
     1021 atataccaag acatgcccct cggaagaata cccggtctct tcgagcaatt ccctgcgggc
     1081 ggcttcaatg gcggtttcgc cgatctccat tccgcctgca ggcagttccc attcaaccgt
     1141 gccggtggca tagcgcggga tgaggacgaa gataacctca cctgctgtat tttccatgat
     1201 cgcagtaaca gcttcgtgct caaaatccag caggtggaat ttatcgatca caaagccgtt
     1261 tgggaatttg acagtgtctt tgtacaggtt cacccacctg ctttgataca ccacctctct
     1321 ggccagttgt tgcggcgtta aatgttggtc tgggttttgt ttggggttta ggtcagtcat
     1381 attgtctcgg atgccgttaa gttttttgtg gagaagcagg tttcgggtaa tggtgaaacg
     1441 cgtttttcgg gtggattccg ttaagatggc agggtcaatg ttacgggtgg tgtgggtact
     1501 tcggggaaaa gtcctcaaaa aaagtggaaa accagtgggt ttccctgtgg ataactctgt
     1561 tgattctgtg gataactggc aggaatggct ccgattttgt ggaaaaatgg cggattctct
     1621 gtggaaaaat ggtctttttt atgtcaaagc ctgtggataa cccagggaaa gcagtggaaa
     1681 acctgctcaa aacctactga taactgcgga ttttctgtgg ataatcctgc tgatattgtg
     1741 tgtaaaactc gttcaggcct gtggaaaact cgggtaaagc ctcgcccaaa tctgtggata
     1801 tcttgttgac agacaggcat aagttgtggg aacggcgggc attaaggctg gaagtgttga
     1861 tggatttggg ggagatttcg ccttcacgca ggccgaactt gcgggcgatc tcgaccccaa
     1921 actggtattt gctcatggcc tgcggaccaa cgaggtgata caagccatgc agtcccctgg
     1981 caagcatttt gacgagggta cgggcagtat ggttgacaag catgggacag aatttcacgt
     2041 cggtaaagcc gctcatgctt ttgttgtagg tcaggttgtg gaaaaagaat tcggcgagac
     2101 tgcgtcggcc ggtgaggctc cacccataaa aattgactcg tgtaacgatg gcgttggggt
     2161 cttctgcgag gacggctctt tcgccttcca gtttggtttt ggcgtacaga ctcagtggat
     2221 tgggttcgtc ttcttctttg tagaaggcaa gcgaagttcc atcgaagacc gcatcagtgg
     2281 aaatgtggat aaaggcagtg ccgcgcaact tgcaggcttt ggccagttgg gcaggcatat
     2341 cggtgttgag gcggcgggca aggtcgggat tttcttcgca ctcttcgagg ttggcgagtg
     2401 cggcgcaatt gatgacccaa tcggggcggg cggtttcgat gatggaatcc accgcaccgg
     2461 gggcaagcag gtcagttttc aagatcggga atggggcatg agccaacttg ccgcgatcca
     2521 cacctacgat ctcatgggta tgcatggttt cctgcgcgag gttaatgccg agtaatccgc
     2581 ttgcaccggt gatgaggagt ttcatgtgtc gggttccagg ttttaggttt nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tccgcttgca ccggtgatga ggagtttcat
     2761 gtgtcgggtt ccaggtttta ggtttcaggt tccaagtttc aggttttatg agcggtttat
     2821 cctaaaacct ggtgttctga cattttatcc ttcgagcttg ccttgggcaa acaactcctc
     2881 aaatggggcg atgtactttt taatgccttc aatatcaagc cattccgaat tattgtcgct
     2941 ggagtaatag gtgccttcgt ttagtgactt gcccttatcc tgccacgaat aaccgaacca
     3001 cgtagcttcg gcaggctgaa ccacatacat cttttcgagt tcgatggtgt gacgggcctc
     3061 gtcttcagag atgagcatct cgtgtaattt ctcgccgggg cgaatgccga tgatattgat
     3121 ctcggcatcg ggagcgatgg cacgggcaag gtccacgacc tttgtgctgg ggatcttcgg
     3181 cacaaagact tcgccgcctt ccatttgttc gatgcaatca atgacgaaac ggacaccctg
     3241 ttcgagcgaa agccaaaagc gggtcatgcg gtcatctgtg atggtgatct tgccgccgct
     3301 gcgttgtttg aggaatagcg gaacgaccga accgcgtgaa ccgacgacat tcccgtatcg
     3361 gacgcacgaa aatctggttg ctgaaccccc ggcataggcg ttgctttgaa tcgtcagttt
     3421 ttcggcagcc aacttggtgg ccccatacag gttcgcaggg ctgaccgcct tgtcggtgct
     3481 gaccgtcaac acttttttga cgcccgcatc cagcgcggct tcgaccacgt tggcagtgcc
     3541 catgatgttg gtcttgatgg cttccatcgg gttgtactcg caggcaggca cctgcttgag
     3601 cgccgccgca tggacaacaa tatccacccc atgcatggca cggacgagtc gctcgcggtc
     3661 acgcacatca ccgatgaaat agcgcagacg cgggtcgttg aatccgccta cctgcatttc
     3721 gtgctgcttc aattcatcgc ggctgaagat gatgaccttt ttcggttgtt tctcttcgag
     3781 caggatcttc gtaaattttt taccgaacga gcctgttcca ccagtgacga gtacaacttg
     3841 atttttccag tccatgattt ctccttgata tcggtagggc aggttgacaa cccgccagtg
     3901 tttatatttt tcaattacat gccaggctga aagcctgacc tacaaggctt tgaacaagcc
     3961 agagtctttc gaaataaaaa tctcaccgtc ttttttcagc acggcggcga attctttttt
     4021 aagcgtttca aattcaggtt cggaaatatc ctcagcaggg atggaggaac ggatgtagtt
     4081 catcaacggg tcaagttctg tgacacgcag gttatcaaca tattgcgatt tttccacatt
     4141 tggaaaaacc tccagcaatt gggcaagtcc gctttcgagg gtaaatgggt tggtgaacat
     4201 atcggggcgc ggatttttgt tcacacgctg cagccagccg tacatttcct tcatgtgatt
     4261 cgccccgacc gtggaagcga tcaaacagcc gtcactcttg agcacacgcc tgatctctgt
     4321 caaagccttt ttacggtcag ggacgtgata gagcatgtga ttggcgatga ccgcatcgaa
     4381 ggttccatcg gcataaggaa tggattgggc gtcgatcttc tcgaacttga agctgcgtcc
     4441 cgttacgacc agattccgcc acgctgaatc aagcatcccg tcggaaagat cggagagggt
     4501 gatggtccag cctgcgggga ttcgatccgc gcattgattc cacatcgtgc ctgcgccgca
     4561 gcccaattcc aacacatgag catcagcagg caatgtgacc agcgcatcaa agacccaatt
     4621 gaaccagccc tgcggattcg tgctgaacct ttgatggatc gcgatgcgcg catcgaggtt
     4681 cgcagaatcc ttgtactggt ctgtggtgag atattgctgg tcggtaaatc tttgcatttt
     4741 atttctttac cacgatcaaa gggtactgcc cgttttctgc gaagaatttc gggaagatac
     4801 cttcgagcca gaagataaat ttcaaaaacg cgcgcccgcc gaagggacgg acgaaattgc
     4861 gcaggatgcg ggtgctcagt ccgcgccagg gtttgagttc gatggtcatc ttcgagccga
     4921 tctcacgctt gagccatgcg ggagacattt tttcaacgaa ggttcccgcg ggggcatctt
     4981 cgctcagcca ttcctttttc ttcttcgcat cgcgcccagt gactttgcgc agcagaccga
     5041 tgaacggttc tgccatgcgg ctgatgagcg ggtcactcca cccgttgacg atggcggcgg
     5101 tgcgaccagg cgcaagcacg cgatggatct cggcatacgc gcgcggatgt tcaggcaaag
     5161 caaggtgatg gatggcatgc atggaaactg cgccgtcgaa agcatctttt ttgaagggca
     5221 gattcgccag gtcaccaacc acgaacaagc cgtgatcacc gattcgagtc cgcgcttcac
     5281 gcagggcttg aatggaaata tcaaggcaga cgcgtttttc gtagcccgct gaataggtct
     5341 tgtactcttc atattggaca ggaccagagc ccgcgtccag catgaaccgc cccgtgggga
     5401 tcaatccatt catcacgcgc aggcgcgtct tgtggatgta ttcgcgcgag acggggcgca
     5461 ggtcttcata acgggcattc tgataatttc cgtcatcttc ctgcatccag ccgatctcgt
     5521 cgtagaactc gcgtactttt tgtttggttt cgttggtcat atcttcctaa cggatcgcgg
     5581 acttcagtcc gcgttttgta tttttgcgga ctaaagtccg cactccgatt tatttccagc
     5641 taaacggctc acccaccaga aagtcaaagg tgtccttgaa ctgcgccttg ccttcttcgc
     5701 tcaacacact ttcgagtgga tgctcggcga ggtcgtccca gaaattcatc aaacgccagg
     5761 ggaagggacg cgggtactcg aagaaaaatc ggtaggcata attccacgcg aactcgatct
     5821 gcttttcggt catttgatga gcggggatgt cgctcaaaac tttttcaagg gtggcgtagt
     5881 attcttccca tgtattgggg tcaatggtga tgccgcgtcc gcggtagtgg gtccgaccac
     5941 aagagatgac ggggcgtccg ttcattgtgg tttcaagccc gaccgtggtg gtgtacgcca
     6001 gcccgacatc cgcgatctcg atcaggtcat acgtgttgac cttgtccaac gcgccgatca
     6061 catggatgtg gctggggagt tcgggcaaag catccttcac gaccgtcccc atggatttgg
     6121 cttgcggcac caacttctca ccaggatgca cacggatgac caattgcaca tccgtccgcc
     6181 tggcaaaata ttgaacagtc ttcgtgatcc actcggtcat ggatgaagca aagatgtccc
     6241 gcccgagagt gaggctgtca ccgagcacat tcgcggcgag catcaccact gggcggtcat
     6301 cgaggttgag cattttgcgg gtctctgccg cgccttgcga agagacatac tgccagaggc
     6361 gttttgattt gccccacaca cgagcgccgc ggcgggcatt ttcaaggtct gctacgcgct
     6421 cgaacatggc atcggtcatg ggcagggagc aacgcgcatc cacgagataa tccgtctcct
     6481 gctgcatgat ggaggaattt tgcgccagcc agatctgttc gcgctgatcg ttgaattcgt
     6541 aggtgaccgc atcaatgccg aggtggcgcg ccacacggaa gacaattccc atttcgagga
     6601 tcaagccgtt gggaatcaaa accacatcgg gcttttccgt ctgcatgaat tgaagcgcgg
     6661 ctttggcggc aaaggtgttg cgttcgatgc gcaggtcata gagcgcgcgg tcgcctgcat
     6721 cgttcatgtc cacttcttcg cgcatcaggg tgtattgcac atcccaaaga gaaacttctt
     6781 tgatatccgc caggatcttc tctggcaggt cgtcatgctt cactgcgcgc agcgtgccat
     6841 gcgaaacccg cgcagaagat tgcatatcca acatcgaaac atgcttcacc aacggcgaca
     6901 acaccgcaag agaatcatgc gtgtgcagca cacgctgacg ctgggtgaac ttatcctttt
     6961 ccttgtgcca ctcggaataa ggcagggtca acaatgtgac atcgtgcccc aagcccgcca
     7021 gcaccaaacc aagataggca gattgctcga tccaatagtg cagggtggca aagaacaaaa
     7081 cttttttacc gcgaggcgcc ttctcagcga acggtttgac cacttccacc gccgcgggca
     7141 gcgacttttc caaacgcccg aggttgtagt gatctttgcg cggacgattc ttcgaccgaa
     7201 gcatccagtc aatttcggca gtgaagggca ggtcgcccag aaggttgcgg agggtgtttg
     7261 tcatagggtt ccaaggtgaa gaggttgaaa gttgaaggtt ttaactttca accttcaact
     7321 tataactttc taaccaatct tctcgatctt ccaattcaat cgcggacgaa caggtcccac
     7381 gcgcggctcc gcccattgcg tgccaggcgc gccagaaata tccacgttga acgccagcgc
     7441 attttcatcc atgaagtagc ggcgcacacc gaacgaactc gcgttcacgc ccacagtata
     7501 gcgaccttca ttgaaaatat cggcgggcaa ttccgcgcgg ctgatgtaat gacccgcatt
     7561 gcgcgagtcg aaattttcat aaaactcggg gctgtcggta tcgaaggagg tgaaaacata
     7621 ttcaccgcgc atcgtgctga cgtaaagtcc cacccgcaga cctgtgacgg gtgcagataa
     7681 tttgtactcg aattcgaccg tgactggttc cgtggaacgg atcgtatcga cgaccttccc
     7741 gcttctttct ttaactttga ggctgatcgg cgtaaaaggc attgccgccg ctgggacatc
     7801 ttccgcgccc caggtgcgct cgcccgcctg cgattgcccc gatgacaaat aatagtccac
     7861 ggcttcctga gtcggtccac gcatgatgag ctgacctttg ttcagcacga tcgcttcctg
     7921 cgtcagacga aggatcgcag acatgttgtg actgacgaac aagaccgtgc gtccctgttg
     7981 ggcaacatca cccatcttgc cgagacattt cttttgaaat tcggcgtcac ccaccgcgag
     8041 cacttcatcc acgacgagga tttcaggctc aaggtgtgca gccaccgcaa acgcaaggcg
     8101 caaatacatg cccgacgaat aacgcttgac aggggtgtca atgaattggc tgacctcgga
     8161 aaaatccacg atctcatcga acttcgagtc gatctccgtg cgcctcatgc caaggatggc
     8221 gccgttcatg tagatgttct cgcgtcccgt cagctcgggg tggaaacctg tacccacttc
     8281 gagcagcgag ccaacccgtc cgcgcacaga tacggtgccc gtggtcggtt ctgtcactcg
     8341 ggagaggatc ttcaacagcg tggacttgcc cgcgccgttg cgccctacaa tgccgagtac
     8401 cttgccttct tcgaggtcaa aggaaacatc cttcaacgcc cagacaaaat tctggttcaa
     8461 gcggcggtct gacctgccga tcatcgcctt cgccagacga atgggcgcag agaccgtatc
     8521 cacgatcaca tcacggagca tgttatagcg gaatttcgtc tccgctgctc cgatcttgta
     8581 ttgcttgcca atgttcttta cgctgattgc tgttgtcata ggtggtatca ggggtcaagt
     8641 ttcaagtatc aggttatttt gtgagcgagc gtttgaaacc gccaacaagc gctttggttt
     8701 tccgtgcttg ctcataatgt atatcaaact cattttggtc aatgtattcc acatccaaag
     8761 ctgcatagag catggattgc acttcaacag ccgagcggcg ggcaatccct agaaaacggg
     8821 aaaattcgat tttagattca caatcaaaac cctctgcaat attattcatc acagaaacgg
     8881 acgcgcgcct tatttgatct cgcattccaa aatctttaga aaacttgtct ttatttgtaa
     8941 gttgataaac catctttacc agtgttcgtg cttcctgcca ggcttgaata tcttcaaatt
     9001 tctggatcat tgccatgata ttctccatta ctcaggcggc ttgacacgtg acacctgaaa
     9061 cctggcactt tatatcgtat ccgcaaaggt cttttccatg cttctgaaat agaagagacc
     9121 agagataaag gtcaaaaccg agatgccgac agaaacccaa agggagaggt cgggaccatt
     9181 gcctgtgccg agcaatgccc agcggaagcc gttgaccacg cccgccatcg gattgaggga
     9241 atacacgacc tgccattttt cagaaatgac agaggcagaa tatgccacgg gtgtggcaaa
     9301 cagccaaaac tgagtcaaga acggcagcgc ctggttcaca tcccgatact ggacgttgat
     9361 cgctgacagc cagagagcaa ccccgagcgc
//
LOCUS       DAOQJC010000145         9245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_3963_length_9245_cov_8.184875, whole genome shotgun sequence.
ACCESSION   DAOQJC010000145 DAOQJC010000000
VERSION     DAOQJC010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9245
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3963_length_9245_cov_8.184875"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLE39_16560"
     CDS             <1..190
                     /locus_tag="PLE39_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69998.1"
                     /translation="MMAIGGLFIPEAKESVEMMYEFEKPFIVDSSKFEKTFGMQATLM
                     KDAIKATVAWYKSHPHKK"
     gene            229..819
                     /locus_tag="PLE39_16565"
     CDS             229..819
                     /locus_tag="PLE39_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE69999.1"
                     /translation="MNTIKWIQKYSTGRNVAIFFSLNMVFYLAILLYSIPLVGRSAPS
                     MKLFDVSPSGYTKEYAVTLLTAIGSAGRDLYLSLQLPLDFVYPGLFIVSYSLMFAWLL
                     RKNYDLRSKIHYVLYLPIFAGLFDYLENVFVILMLKTYPDLSAGLVSAASFATVAKSI
                     LSSVFFTLLLLSIVQTLWRAAFKRADTAPVPDSRRV"
     gene            complement(828..1922)
                     /locus_tag="PLE39_16570"
     CDS             complement(828..1922)
                     /locus_tag="PLE39_16570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE70000.1"
                     /translation="MKFALVHEWLNVYGGSERLLTEILQLYPQADLHALIHNPVNLQN
                     TPLAGRRVKTSFLQNIPHVEKLYRGLLPLMPLAIENMNVRNYDVVLSISHAVAHGIKT
                     HKGQTHISYVCTPMRYAWHLQDDYLHLHHLDKPLVGAAARLTLSLLRRWDRESASRAD
                     HLLAISQWTAERIRQAWGRESQVIHPPVNVNRFSPTDEREEFYLLVSRLVPYKMTTEI
                     IMAFNELKLPLLIAGDGPEMPRLQKLAGSNVRLLGHQPDSIVTDLMNRAKAFLHMAVE
                     DFGIAMVEAQAAGCPVIAYEKGGAAEIVRNGETGLLFTQQTAKALVEAVVKFKSLKLN
                     SKAASMNAARFSTERFTKEFLSYMENLCGN"
     gene            complement(1919..2926)
                     /locus_tag="PLE39_16575"
     CDS             complement(1919..2926)
                     /locus_tag="PLE39_16575"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNE70001.1"
                     /translation="MNKIAVNGRCLTRRITGVERYAREISKRLAGSHILQPQNGTGQV
                     AGNLWEQVILPGQLKHDEILWSPANAGPWLVKNQVITIHDASVFDHPEWFKPAFAAWT
                     RLSWQMLARRVRAIITVSNFSCERLKHHLKIPAEKIHVVHNGVGKPFAPQAQPHIDAV
                     RSKYGIQKPYFLFVGTIEPRKNLGGLLQAWNRAGITTHELFIAGARGRVFQPISGAPN
                     IVYNVHDDDLPALYSGAAAFIIPSFYEGFGLTALEAMACGTPVIASRTTVFPETLGEA
                     AQFIDPYSVNQLSDAMRAILEDSALAATLRKRGLEHASRFSWDESARKTQEIICAAGG
                     S"
     assembly_gap    3015..3114
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3115..5702)
                     /locus_tag="PLE39_16580"
     CDS             complement(<3115..5702)
                     /locus_tag="PLE39_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70002.1"
                     /translation="MIPRRVLLAVLFASLLHGFFVLTARYRLSYDAYNHMLFADHYAA
                     DWFSLWETRWYTGFSVVSYPPLTHQLIALFIPILGFDAAYALVLWVVTSLYPLGVYAF
                     SRIFTSRGASSYAALASALLLPIYVTAHIFGQLPFLTATLFTFFGMAALAKYAREGGL
                     RNLLLAVSFTATTMAAHHATLMIQPFFIFSIAVFQLGKNTWKHLVIRLGTFAALAIPA
                     GLAVIWPFWVWGTQQELQTPIDHLSRHNFLAEPFARTIFFWPFYFPLIAVVPFIFYKW
                     GKKFIGLQFSFVILFILGLGGTTKLPALLFGKSWEWLTYDRFAFWASLSLLPFFGILF
                     IKFHRRMKTRFALNLKPATLRKNLIPTLTFLIIASGAIGSWFTPKFFPIQPDPVDMQP
                     IVDFMAAEDHSYWRYITFGFGDQFAYLNLLTKNATTLDGSYHTARSIPELRESGIGQI
                     DTAFWTQQGIPAVAPILQASGKYGVRWGFVNLRLFVPELKKNGWEFVKYLPNGIQVWE
                     NPDFTFQPPIIPPEDPFESFSWGILPMLSLATTLALGTVTFWQERGIRLVQNAYAVVV
                     SIIPLSLGLWYYKTIFEFKHTQVYFTYDHALFFLSDALALTAILLWLAATPQVRLNLF
                     HKLFFALCSWITLSAAWSLDWRTSLYIAGHFWLISLLILSLQDQPKAWTGIFAGAILA
                     LTLQGIIGWSEFRSQSTQVLERLNLPWPGLISASSKGAGKLKYANGENFLRVYGSFPH
                     PNILGGFAVFGIAAATALLLRKRESLLIWAAPIVGAVVIALSFSRSAWLGASAFFIVL
                     LLKAKFLEKKKLWAILALLALAFAAVMFPLRELFLSRTTAPTSNTEKFSLVGRAWLSQ
                     QAWSYI"
     gene            complement(5699..6886)
                     /locus_tag="PLE39_16585"
     CDS             complement(5699..6886)
                     /locus_tag="PLE39_16585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE70003.1"
                     /translation="MHLTVVSPFPPTITGIGQYGFHITRAIEQAGLFSRITVLAGAKT
                     ESHNSRLLNNAEIDYCWRPDDLRTRGVILSRLKHLQPDLVWFNLGASVFGRSPLANLS
                     AMFSPMLAQRMGLPTIVTLHELVELADLRALNAPGGFLAPLGARLLTEIATKADLVCL
                     TMQHYVDWLSARNINCVHIPIGSYHEPELLEETDEQELLFFTTLAPFKGLEILLKAFK
                     ELKNDFPKLRLSIAGADHTRFPNYSKDLRKQCDEANSIRWLGQIPEEKVKDLFRSAQI
                     VVLPYTASTGSSSVLYQSATWGRAIVASDLNEHKHLARENGLQIQFFENGSVQSLKDS
                     LCNLLKQNSLRKSQTLHNFNAIQKLTPSETCRHYIQAFNSALESCRSQKRIIIPRKEM
                     ELL"
     gene            6990..7850
                     /locus_tag="PLE39_16590"
     CDS             6990..7850
                     /locus_tag="PLE39_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70004.1"
                     /translation="MDLKQAVLETLAYSDVFDHPLTLGELRKFLVAPASQAELQACAA
                     GMESVAYANGFYHLAHRTDILEIRKLREKNSKSAFKRAEFYGRILGRLPFIRMVAMTG
                     SLAMLNLSKNKDMDYLIVAKAGRVWTARAFALLFGRLARMVGDVICPNVIVSENSLAW
                     EAKNLYTAREFAQMIPLSGWGVYRRLLNVNAWVADFLPNAFSDLDESPRENSSLTQRL
                     GESFLNRGLGDAFEKWEMTRKIARLQAQEGYGTETNFSAEICQGNFDHHGTWAMKAFQ
                     DRVSSLQVEK"
     gene            7847..>9245
                     /locus_tag="PLE39_16595"
     CDS             7847..>9245
                     /locus_tag="PLE39_16595"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE70005.1"
                     /translation="MTQILFGQSYYLRFDPKLWDAMMPYPPLGTLYAASAIRARGYDV
                     ALFDAMLADSEEEWARSLDEHRPEYAVIFEDNFNYLSKMCLLRMREAAFTMIRMAKER
                     GCTVILCGADATDHYSQYLERGADYCLLGEGEETLAELLDQLSAGKDAEGLPGLVSKR
                     STHALRRPDIKDLDALPFPAWDLVDVQKYKKIWLERHGYFSMNMVTTRGCPYHCNWCA
                     KPIWGQRYNSRSPENVAAEMKWLKENFAPDHIWFADDIFGLKPNWVERFAELLHELDA
                     VIPFKCLKRADLVTEKTASALAKAGCKTVWLGAESGSQKILDAMDKGDQVEDIHRAAH
                     LLHENKIEVGFFLQFGYPGETWADVQKTLKMVRECMPDDIGISVSYPLPGTKFFERVK
                     MELGEKQNWVDSDDLAMLYHGPFPQEFYRILHGRVHHEFRLRRAWKKKDLRGMIRTPY
                     YLLGVAKSEYMLRKFL"
BASE COUNT         2147 a         2103 c         2713 g         2182 t
ORIGIN      
        1 catgatggcg atcggtggat tgttcatccc tgaggcgaaa gagtctgtgg agatgatgta
       61 cgagttcgag aaaccgttca tcgtcgattc gagcaagttc gagaaaacct tcggtatgca
      121 ggcaacactg atgaaggatg ccatcaaggc aaccgtggct tggtacaaga gtcacccaca
      181 taagaagtaa gttgccaatt gttgagtttt ttatcgaaga ggttttttat gaacaccatc
      241 aaatggatcc agaaatattc aacaggaagg aatgtcgcca tcttcttttc tctgaatatg
      301 gttttttatc ttgcgatctt gctttactcg atcccgttgg tgggcagatc tgcccccagc
      361 atgaaattgt tcgatgtttc tccctctggg tacacaaagg agtatgcggt gactctgtta
      421 actgccattg gttctgcggg cagggacttg tacttatcgt tgcagttgcc gctggatttt
      481 gtttacccgg gtttgttcat cgtctcatat tccctgatgt ttgcgtggct gctcagaaag
      541 aattatgact tgcgttcgaa gatccattac gtgctttatc ttccgatctt tgcagggttg
      601 ttcgactatc tggaaaatgt ttttgtcatt ctgatgttga agacttaccc tgacctttct
      661 gctggtcttg tgtctgccgc cagttttgcg accgttgcaa aaagtatcct gtcttcggtt
      721 ttctttacgc tgctgctttt gagcatcgtg cagacccttt ggcgggctgc attcaaaaga
      781 gcggacaccg cgcccgtccc cgattcgcgg cgcgtatgag gcgttgacta atttccgcag
      841 agattttcca tatatgagag gaattccttt gtaaatctct cagttgaaaa gcgagctgca
      901 ttcatggatg cagctttgct gtttaatttc aggcttttga actttacgac tgcctccaca
      961 agcgcttttg cagtttgctg tgtgaataac agccctgtct ccccgttgcg gacaatttca
     1021 gccgcgccgc ctttttcgta tgcaatgacg ggacaccccg ctgcctgtgc ttcgaccatg
     1081 gcgatcccaa aatcttccac agccatgtgg aggaaggctt ttgcacgatt catcaggtct
     1141 gtgacgattg aatcgggttg atggcctaac agtcggacat tgcttcccgc cagtttttgc
     1201 aagcgcggca tttcaggccc atcgccagcg atgagcagcg gaagtttcaa ctcgttgaaa
     1261 gccatgatga tctcggtggt cattttgtat ggcacgaggc gcgaaacgag aagatagaat
     1321 tcctctcttt catcggtggg cgagaaacgg ttgacgttta cgggcgggtg gatgacctgc
     1381 gattcgcgcc cccaggcctg acggatgcgt tcggccgtcc attgtgagat ggcgagcaga
     1441 tgatcggctc gggatgctga ttctctgtcc caacggcgga gcaggctcag ggtcaggcgg
     1501 gcggcggccc cgaccagggg cttatccaaa tgatgcaagt ggagatagtc atcctgtaaa
     1561 tgccaggcgt agcgcatcgg cgtgcaaaca tacgaaatgt gagtctgacc cttgtgcgtt
     1621 ttgatgccgt gtgcaaccgc atgtgaaatg gacaggacaa cgtcataatt gcgcacgttc
     1681 atgttctcga tggcgagcgg catcagcgga agaaggccgc gatacagttt ttccacatgg
     1741 gggatgtttt gcagaaagga tgttttgacg cggcggcctg caagcggtgt gttttgcaga
     1801 ttgacggggt tgtggatcaa tgcgtgaaga tccgcctgcg ggtacaactg taagatctcg
     1861 gtcagcagac gttccgaacc cccatacaca ttgagccatt cgtggacgag ggcgaacttc
     1921 atgaaccgcc tgccgcgcag atgatttcct gagtctttcg cgccgattcg tcccatgaaa
     1981 aacgggatgc gtgttcgagg ccgcgtttgc gcagggtggc cgcaagtgca gagtcttcga
     2041 ggatggcccg catggcatcg gatagttggt ttacagaata tggatctata aattgcgccg
     2101 cttctcccag tgtttcaggg aaaacggtgg tgcgcgaggc aatgacgggt gtgccgcaag
     2161 ccatggcttc gagggcggtc aggccgaagc cttcataaaa agaagggatg ataaatgcgg
     2221 cggcacccga atacagggcg ggcaggtcat catcatgcac gttgtaaacg atgttgggtg
     2281 cgccgctgat gggttgaaag accctgcctc tggcgccagc gatgaaaagt tcgtgggttg
     2341 tgatgccagc ccgattccat gcctggagca agccgccgag gtttttgcgc ggttcaatgg
     2401 tgccaacgaa caggaagtat ggtttttgga ttccgtattt cgatctcact gcgtcgatgt
     2461 gtggttgtgc ttgcggcgca aagggttttc caacaccgtt atggaccaca tgaatttttt
     2521 cagcggggat tttgaggtga tgtttcagcc gttcacatga aaaattggaa accgtgatga
     2581 tggccctgac cctgcgggca agcatctgcc aggagagtcg cgtccaggcg gcgaaggcgg
     2641 gtttgaacca ttcgggatga tcgaagacac tggcatcatg gatggtgatg acttgatttt
     2701 tcacaagcca tggacctgca ttggctggag accaaagaat ttcgtcgtgc tttagctggc
     2761 caggcaaaat gacctgctcc caaagattcc ctgcaacctg ccccgtaccg ttttgcggct
     2821 gaaggatgtg gctgcctgca agtctcttgc tgatctcgcg ggcgtagcgt tccacgcctg
     2881 taatgcggcg ggtgaggcag cggccattga cggcaatttt attcatgggc ggcctgcact
     2941 tgtccctgcc aaagcccgag cgcgaggcca aacagcatgc ggcctggcgc aagcgaccag
     3001 agatagtggt cgaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttgatg
     3121 tagctccatg cctgttggct gagccaggcg cgcccgacga gtgaaaattt ttctgtgttg
     3181 cttgtggggg cggttgttct gctaagaaaa agttcgcgca agggaaacat gaccgcagca
     3241 aatgcaagcg cgagaagtgc aaggatggcc cagagttttt tcttttcaag aaactttgcc
     3301 ttcagcagca gcacaatgaa aaaagcggat gcccccaacc aggcggagcg tgaaaaggaa
     3361 agcgcgatca ccaccgcgcc gacaattggt gcggcccaaa taagcagact ctcccttttg
     3421 cgaagcaaca aggctgtggc ggctgcaatt ccaaatacag caaagccgcc caaaatgttc
     3481 gggtgtggga atgaaccgta aacgcgcaaa aagttttcgc cgttggcgta tttcaatttg
     3541 ccagcacctt tggaagaagc gctgatcaaa cctggccacg gcaggtttag ccgttcaagc
     3601 acttgtgtag attgcgatcg aaactcactc caaccgatga tgccttgaag cgtcagcgca
     3661 agaattgcgc ctgcaaagat ccctgtccac gctttgggtt gatcctgcaa agagaggatg
     3721 agcagggaga tcagccaaaa atgcccagca atgtacaaag aagtgcgcca atccaaagac
     3781 catgcggcgc tgagcgttat ccaggagcaa agggcaaaga ataatttatg gaataagttg
     3841 agcctgactt gtggagtggc ggcaagccag agcaggatgg ctgtcagcgc cagtgcatcg
     3901 ctgaggaaaa agagggcatg gtcgtaggtg aaatacacct gtgtgtgttt gaattcaaaa
     3961 atggttttgt aataccagag gccaaggctg agcggaataa tgctcacgac aacggcatac
     4021 gcattttgga cgagtcgaat gccgcgttcc tgccaaaacg tgaccgtgcc aagcgcaagc
     4081 gtggtggcca gcgaaagcat cggcaggatg ccccatgaaa aagattcaaa agggtcttcg
     4141 ggcggaatga tcggcggctg aaatgtgaaa tctggatttt cccaaacttg aattccgttt
     4201 ggcaggtatt tgacaaattc ccagccgttt tttttcaact cagggacaaa cagccgcaag
     4261 ttgacaaagc cccagcgcac gccgtacttt cccgaggctt gcaagatcgg ggcaacggca
     4321 gggattccct gctgggtcca gaaggcggtg tcgatctggc cgattccgct ttcgcgcaat
     4381 tcggggatgg agcgggcggt gtgataactt ccatcgaggg tggtggcgtt tttggtgagc
     4441 aggttgaggt aggcaaattg atcgccaaag ccgaaggtga tgtaacgcca gtaggaatgg
     4501 tcttcggcag ccatgaaatc tacgatgggt tgcatgtcca cggggtcggg ttggatggga
     4561 aaaaatttgg gcgtgaacca tgaaccaatg gcgccgctgg caatgatgag aaatgtcagg
     4621 gttgggatca ggttctttcg aagcgtggca ggtttcaggt taagggcgaa gcgggttttc
     4681 atccgcctgt ggaatttgat gaagaggatt ccaaagaacg ggagcaggct gagcgatgcc
     4741 caaaatgcga agcggtcgta cgtgagccat tcccagcttt tgccgaagag cagggcgggg
     4801 agtttggtgg tgccgccaag gccgaggatg aaaaggatga cgaatgaaaa ttgcaaaccg
     4861 atgaattttt tgccccattt gtaaaaaatg aatgggacca cagcgatgag cggaaaatag
     4921 aacggccaga aaaaaatggt gcgcgcaaat ggctcggcga gaaagttgtg acgggagaga
     4981 tggtcaatgg gggtttgtag ttcctgctgt gtgccccaaa cccaaaacgg ccagatgacc
     5041 gccaaccccg cagggatcgc cagcgcggca aatgtgccca gcctgatgac gagatgcttc
     5101 catgtgtttt tgccaagttg aaagacggca atggagaaaa taaaaaacgg ctggatcatc
     5161 agagtggcat gatgagcagc catggtggtg gcggtgaatg agaccgccag taaaagattg
     5221 cgcaggccgc cttcccgtgc gtattttgcg agggccgcca tgccaaagaa ggtgaacagg
     5281 gtggccgtga gaaatggaag ctggccgaaa atgtgcgcag tgacgtagat gggcagcaaa
     5341 agcgccgatg ccagagcagc gtacgatgaa gcgccgcggc ttgtgaagat tctgctgaag
     5401 gcatacacac ccagcggata caaggaggtg accacccaaa gcacaagggc gtatgcggca
     5461 tcgaagccga ggatggggat gaagagtgca atgagttgat gtgtgagcgg cgggtaggaa
     5521 accacactga agcccgtgta ccagcgtgtt tcccataacg agaaccaatc tgccgcgtag
     5581 tggtcggcga agagcatgtg gttgtaggca tcgtaactca ggcggtagcg cgcggtgagg
     5641 acgaaaaatc cgtgcagcag agatgcgaag agaacagcca gaagcacgcg gcgcgggatc
     5701 atagcagttc catttccttg cgcggtatga tgatccgttt ttggctgcgg cagctttcca
     5761 gtgcggaatt gaaagcctga atgtaatgac ggcaggtttc gctgggagtc agtttttgga
     5821 tggcgttgaa gttgtgcagg gtctggcttt tgcgcagaga gttctgcttt agcaggttgc
     5881 agagcgaatc tttcaggctt tgaacgctgc cgttttcaaa gaattggatc tgcagaccgt
     5941 tttctctggc gaggtgcttg tgctcgttca ggtcagaggc aacgatggca cgcccccagg
     6001 tggccgattg gtacaaaacg ctggaagacc ccgtggaagc ggtgtaaggc agaaccacga
     6061 tctgtgcgct gcggaagagg tcttttactt tttcttcggg aatctgccca agccagcgga
     6121 tgctgttcgc ttcgtcgcat tgctttctga ggtctttgga ataattggga aagcgggtgt
     6181 ggtctgcgcc agcaatggaa aggcgtaact ttggaaaatc atttttcagt tctttgaagg
     6241 ctttgagcag aatctccaga cctttgaagg gagccaatgt ggtgaagaac agcaattcct
     6301 gttcgtcggt ttcttccaaa agttcaggct cgtgatacga gccgatgggg atatgcacac
     6361 agttgatgtt gcgggcggag agccaatcta catagtgctg catggtcagg cagacaaggt
     6421 cggctttggt ggcgatttcg gtcaacagcc gcgcgccgag cggggcgagg aatccgcctg
     6481 gggcattgag tgcgcgcagg tcggcaagct ccacgagttc atgcagggtg acaatggtgg
     6541 gcagtcccat tctctgtgcg agcatgggcg agaacatggc tgaaaggttt gccagcggtg
     6601 acctgccaaa gacgctcgca cccagattga accagaccag atcaggctgt aagtgtttca
     6661 agcgcgacag aatgaccccg cgcgttctca ggtcatctgg ccgccagcaa taatctatct
     6721 ctgcgttgtt cagaagacgg gaattgtgac tctcggtttt cgctccagca aggacggtga
     6781 tccgagagaa caaacctgcc tgttcaatgg cccttgtgat gtgaaacccg tattgtccaa
     6841 taccggtaat tgttggaggg aatgggctga ctacggttaa atgcataaga agctggaatt
     6901 attccattaa tgtgagaatt agacaacgcg tagcacggca actatacgtt gcaggatttt
     6961 aacttgcaaa gtatagttcg tctcctatta tggatttgaa gcaggctgtt cttgaaaccc
     7021 tcgcatattc agacgtgttc gatcatccat tgaccctggg tgaactgcgt aagtttttag
     7081 ttgcgcctgc ttcacaggca gagttgcagg cttgtgcggc gggcatggaa tctgtggctt
     7141 atgcaaatgg tttttatcac cttgctcatc gcaccgatat tttggaaatt cgaaaactgc
     7201 gtgagaaaaa ttccaaaagc gccttcaagc gagccgaatt ttatggccgc attttgggtc
     7261 ggctgccttt tattcgcatg gtcgcaatga caggttccct ggcgatgctc aatctttcga
     7321 aaaacaagga catggattat ttgatcgttg ccaaagcagg gcgggtgtgg actgcacggg
     7381 cctttgccct gctgttcggc aggctggcgc gcatggttgg cgatgtcatt tgcccgaatg
     7441 tgatcgtttc ggaaaattca ctggcgtggg aggcaaaaaa tctttacacc gcccgcgaat
     7501 tcgcgcaaat gattccgctc agcggctggg gtgtgtatcg ccgtttgtta aacgtcaatg
     7561 cctgggtggc ggattttctg ccgaatgctt tttctgatct ggatgaaagc ccacgcgaga
     7621 acagttccct gactcagcgg cttggcgaat cttttcttaa ccgtgggctg ggtgacgcct
     7681 tcgaaaagtg ggagatgacc cgcaagatcg cccgcctgca agcgcaggaa ggctatggca
     7741 cagagacgaa tttttcagcg gagatttgcc agggtaattt tgaccatcac ggcacgtggg
     7801 caatgaaagc ttttcaagac agggtttcaa gtttacaggt tgaaaaatga cacagatcct
     7861 ctttggacaa tcctactatc tgcggtttga ccccaagctt tgggatgcca tgatgccata
     7921 tccgccgctg gggaccttgt acgcggccag cgccatccgc gcgcgtggat atgacgtggc
     7981 gctcttcgat gcgatgctgg ctgattcgga agaagaatgg gcaaggtcgc ttgatgagca
     8041 tcggccagaa tacgcggtca ttttcgaaga taatttcaat tatctttcca agatgtgcct
     8101 gctgcggatg cgcgaagcgg ccttcacaat gatccgcatg gccaaagagc gcggatgcac
     8161 ggtcatattg tgcggcgctg atgcgaccga ccattacagt caatacctcg aacgcggcgc
     8221 ggattactgt ctgctgggtg aaggggagga gactcttgca gaattgctgg accagttgtc
     8281 ggcggggaag gatgcggaag ggcttcctgg ccttgtatcc aaaaggtcaa cacatgcctt
     8341 gcgccgtccc gatatcaaag acctggatgc gcttccattc cctgcctggg atctggtgga
     8401 tgtgcagaag tataaaaaga tctggcttga gcggcacgga tatttttcga tgaacatggt
     8461 gacgacccgt ggctgcccgt accattgcaa ttggtgtgcc aagccgatct ggggccagcg
     8521 ttataactcg cgctcgcctg agaacgtggc ggcggagatg aagtggttga aggagaattt
     8581 tgctcccgat cacatttggt ttgccgatga tatttttggc ttgaaaccaa actgggtgga
     8641 gcggtttgcg gaattgctgc atgagttgga tgcggtcatc ccgttcaagt gtttgaagcg
     8701 cgccgacctt gtgaccgaga aaacggcttc ggcgcttgcg aaggcaggct gtaaaacagt
     8761 gtggcttggc gcggagtcgg ggtcgcagaa aattttggat gcgatggaca aaggcgatca
     8821 ggtggaggat attcaccgcg ccgcgcattt gctgcatgaa aacaagatcg aggtcggttt
     8881 ctttttgcaa tttggctacc ccggtgagac ctgggccgat gtgcaaaaga ccttgaaaat
     8941 ggtacgtgag tgcatgcccg atgacatcgg catttcggtt tcctacccgc tgcctggaac
     9001 gaaatttttt gagcgtgtaa aaatggaatt gggcgagaag caaaactggg tggattcaga
     9061 tgaccttgcc atgctttacc acggaccgtt tccacaggag ttttaccgca tcctgcatgg
     9121 acgggttcac catgaattcc gtctgcgccg tgcatggaag aaaaaagacc tgcgcggcat
     9181 gatccgcacg ccgtattatc tgttgggcgt ggcgaagagc gaatacatgt tgagaaagtt
     9241 tttat
//
LOCUS       DAOQJC010000146         9078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_4071_length_9078_cov_10.098083, whole genome shotgun sequence.
ACCESSION   DAOQJC010000146 DAOQJC010000000
VERSION     DAOQJC010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9078
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4071_length_9078_cov_10.098083"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PLE39_16600"
     CDS             <1..949
                     /locus_tag="PLE39_16600"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNE70006.1"
                     /translation="MRKGLLLYNPAAGRYPVRRYVRGIMHPLKEAGWSIEIAETLSGT
                     HATQTAHQAAAEKYDAVFAIGGDGTVGQVASGLLDSDTALAVLPAGTTNVWAIEQGQK
                     PFSWFNWWSLRENAKLLANVDVQQVDVGMCNDQPFLLWAGVGLDAHTINKIEPRHRFF
                     KHISVPHFFATTVWEATFWHGLDLRVWTDDKLVEGHYLVAVATNIRHYAGGMSVLSPE
                     ALLDDNEMDMWLLSGNSVADALRHFFDLVAGRHLTSDQARRLPFHTARVESDAAFSIQ
                     VDGDPALGSSHADITIKHRALKILMPKDALYLLKNPHKH"
     gene            999..1475
                     /locus_tag="PLE39_16605"
     CDS             999..1475
                     /locus_tag="PLE39_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70007.1"
                     /translation="MDFKPYFNRWTIFGAIAFAGILLIVTLIIIGWTSPRFTPDVGFA
                     PADLTMIPAPTHTPNAPLVPTLDPSLVTPTLAPGTIGIGAYVQISGTEGEGLRIRSIA
                     GLTGDTVFFGGESEVFLVRDGPQTADGYTWWYLVAPYDENRAGWAAADFLAVVPSP"
     gene            1569..1919
                     /locus_tag="PLE39_16610"
     CDS             1569..1919
                     /locus_tag="PLE39_16610"
                     /inference="COORDINATES: protein motif:HMM:NF017922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNE70008.1"
                     /translation="MSEKPTGVTANKSKKEFTVTWDDNHTSVYPFGLLRAACPCASCR
                     GGHENMKSEPDAEVFTRQMEDSQSTRLANVVAVGSYALTIVWEDGHDYGIYNWHYLRA
                     LCPCAECRGKAKKQ"
     gene            complement(2021..3853)
                     /locus_tag="PLE39_16615"
     CDS             complement(2021..3853)
                     /locus_tag="PLE39_16615"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE70009.1"
                     /translation="MAEDRPDDTMFQDAVDALRRGDKARAKELITLLLKADQNNPTYW
                     IWLSAAMDSTKERIYCLQTALKLDPENGTAKRGLILLGALTPDESIQPFPMNRPRAWE
                     EKLLLANEKPKEKGFKAFARSPITRLAFVLLIGAGLVSAVIFGFILPRRSNIRPTETN
                     TPGPSPTFTTTPTLFGATAPPTKAVIGPTPLWMFLPQTYTPTAVYVNTPRAAQSGDQY
                     RLALAAYAQENWDEYITNMQIIASLEPEAADVPYLIGDAYRFKGDADSALDFYNEALK
                     INEDFGPAYLGLARARLLDNPKANVDALFEQAIDLDPDFGEVYLERARYNIINNDPEA
                     AIVDLDRADKLMPNSPVVYMAYADAYLAMDDKKGALEAAETAYSLDITNLEVYKLLGK
                     LHIEGGEYARAIEALDVYAVYETEDDQVFALLGQAYYEMGDYETATSNFEKAEAINKN
                     GLRKFLVYKGLANLELNNLDAAVEDLEKAASADDTSFEINLGLLRAYYLQEKFGSAFL
                     KAEALKSLAETDEETAVMLYWHALVQEKRGEAREAIKDWKALLALDADVMTEQMRADA
                     ELHLKTMVTPTNTPKGGTTKTATPGKTPTPKGGTGTPTPTPTPK"
     gene            complement(3863..4438)
                     /gene="amrA"
                     /locus_tag="PLE39_16620"
     CDS             complement(3863..4438)
                     /gene="amrA"
                     /locus_tag="PLE39_16620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNE70010.1"
                     /translation="MNDQLTDGEKQILLRLAREAMDHAVRGMKLPPLENESLSPRLRE
                     NGASFVTLTIHGELRGCIGALEAYQPLVDDVREHAIAAALSDPRFPPVGENELNRIKL
                     EVSRLTVPHLLEYSSGEELPVKLRPHVDGVILKDGFRRATFLPQVWEQIPDAEDFLDH
                     LCSKMGARRDLWRKTKLQVYIYQVEEFHEMN"
     gene            complement(4492..4998)
                     /locus_tag="PLE39_16625"
     CDS             complement(4492..4998)
                     /locus_tag="PLE39_16625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNE70011.1"
                     /translation="MKNTTLMTLDPEKLRSAMRAWSAGVTVVTAVHDGHKHGMTVNSF
                     TSISLEPAMITVSLRTGTRTHELVTKSGAFGLTILSAEQADISNLFSGQVTGIEDRFS
                     NLNTETMVTGSPLIAGGLAWLDCRVVQTFDAGMNTLFIAEVLAAQEANDGPPLIYHNR
                     KYWNLSEI"
     gene            5204..6448
                     /locus_tag="PLE39_16630"
     CDS             5204..6448
                     /locus_tag="PLE39_16630"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNE70012.1"
                     /translation="MSKRIRNILIGLAGLFLLAVLVYQIPYVNSVLSWRIEKFMIYTK
                     NVFDPVGPVPTALPVTPHPITPTSIATATETPAPVIDLTPSITPTATFAPLPAQASIA
                     SPPFEQQTPNNCGPATLSMALHLYGWKGNQADIANVIKPILQDRNVNPEELRYYVRTQ
                     AGWLNMEYRVGGSIETLKRLIAANYPVIVESVTSLDPNDALGPNDDLWAAHYLLLTGY
                     DDNTQTFTIQDSYHGADLSLPYSQLEKEWKPFNNLYIVIYFPQFEDEMKSLLASNWDA
                     NLNRQLALSASETDTAQNPTDAFAWFNYGSNLVYFDRYEEAAQAYDKAREIGLPLRMF
                     RYQFGPFLAYFHSNRNEDLLVLTDYARGVTEMSEEAWLWYGYGLYRNGDYDGALKAWQ
                     KADQINPNFYEDQAKNAISLIQ"
     gene            6500..7450
                     /locus_tag="PLE39_16635"
     CDS             6500..7450
                     /locus_tag="PLE39_16635"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE70013.1"
                     /translation="MKKTLLFLILLLTACQSTPTPAPAPEETVTVAPSETPAPTVVSF
                     TATPEATFTPVPTATPETIKISTADGMTQLLIPAGTVKMGGLDVLLENDEIPAHDVTL
                     DAFWMDQVEVTNGMYNLCVQAGVCRLPVKLNSDNRGDYFGNPEFQDYPVIQVTWFDAN
                     TYCQWAGRRLPTEAEWERAARGDDLRTYPWGGEPPNELNSNANNIVGDVTRVGSYPAG
                     ASPFGVLDMAGNVWEWVADYYSGRYYGESPAINPLGPEHGGLNFLRVIRGGSFQDNMF
                     NLRVSNRGYETGPDPSAFPGEPEYYGRSSVKIGFRCANAD"
     regulatory      7571..7676
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            7750..8964
                     /gene="metK"
                     /locus_tag="PLE39_16640"
     CDS             7750..8964
                     /gene="metK"
                     /locus_tag="PLE39_16640"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNE70014.1"
                     /translation="MSNTFMTSPSLMFTSESVTEGHPDKICDQVSDAVLDACLEQDPR
                     SRVACETAVKTGYVMLLGEITTNAVFNYDELVRKVVNDIGYDSSEKGFDGNSCGVLVA
                     IAKQSGDIAMGVNQALETRDGHKMSDAEVDSIGAGDQGMMFGYACNETPTLMPLPIYL
                     AHKLTRRQSELRKSGAIKWLRPDAKSQVTVEYSKGQPKRIDTVLISTQHSPEVSQNEI
                     TEIITEELIMKTLPENLVDKDIKVFVNPTGRFVVGGPMGDAGVTGRKIIVDTYGGMGR
                     HGGGAFSGKDPTKVDRSAAYAARYVAKNVVAAGLADRVEVQVSYAIGVAHPLSVNVET
                     FGTARIADDKIAALINEHFDLRPGAIIRDLDLRRPIYRKTAAYGHFGRDDIEFPWEKT
                     DKAAALKKAAGL"
BASE COUNT         2212 a         2407 c         2274 g         2185 t
ORIGIN      
        1 catgcgcaag ggacttctcc tctacaatcc agccgcggga cgttaccctg tgagacgtta
       61 tgtgcgcggg atcatgcatc cactcaagga agcgggctgg agcatcgaaa tcgccgaaac
      121 cctcagcggc acccatgcca cccaaactgc gcaccaagcc gccgcagaaa aatatgacgc
      181 ggtcttcgcc atcggtggtg atgggacagt gggacaggtc gccagtggat tgttggattc
      241 agatactgcg ctcgccgttt tgcccgcagg cacaaccaac gtctgggcca ttgagcaggg
      301 acaaaaaccg ttcagctggt tcaattggtg gtcactgcgc gagaatgcaa aattgctcgc
      361 caatgttgat gtgcagcaag tggatgtggg catgtgcaac gaccaaccct ttttgctgtg
      421 ggcaggcgtt ggtctggatg cgcacaccat caacaagatc gaaccgcgcc atcgtttttt
      481 caagcacatc tccgtcccgc atttttttgc caccaccgtt tgggaagcca ccttttggca
      541 tggtctcgat ctgcgcgtat ggacagacga caaactggtt gaaggtcatt atctcgtggc
      601 ggttgccacc aacatccgcc attacgcggg aggcatgtcg gtgctttcac ctgaagccct
      661 gctcgacgac aacgaaatgg atatgtggct gcttagcggc aacagtgttg cggatgcgct
      721 gcgccatttt tttgatctcg ttgcaggtcg tcacctcacc tctgatcagg cgcggcgctt
      781 gccatttcac acggcgcggg tagaatcaga cgcggccttc tccattcaag tggatggcga
      841 tcccgcgctc ggcagttctc acgcagatat caccatcaaa caccgtgcgc tcaaaattct
      901 catgccaaaa gacgcactgt atttattgaa aaacccacat aaacattaac tggagattgc
      961 ttcgggcagg agaaagaacg ccctcgcaat gacacgacat ggatttcaaa ccctacttta
     1021 atcgctggac aatttttggc gcaatcgcct ttgcagggat tttactgatc gtcaccctga
     1081 tcatcatcgg ctggacctct ccgcgcttca cccctgacgt tgggtttgct cctgccgatc
     1141 tgacaatgat ccccgccccg acacataccc ccaacgctcc ccttgtgccc acactcgacc
     1201 ccagccttgt caccccaacc ctggctcccg gcacaattgg tattggcgcc tatgtacaga
     1261 tcagcggaac cgaaggcgaa ggcttgcgca tccgctccat tgcggggttg acaggcgata
     1321 cggtgttctt cggcggcgaa tcagaagtat ttctcgtccg tgatggcccg caaaccgcag
     1381 atggatatac gtggtggtat ctcgtggcac cctatgacga aaaccgtgca ggctgggccg
     1441 ctgctgattt tctggcggta gtcccttcac cgtagttaat taccgcaaag catgccaaaa
     1501 gacccgaaat atttttttcg cgtttacagc acgcttcacg gttcaaaaag tttttataat
     1561 gaggaaaaat gtctgaaaaa ccaacaggcg taaccgccaa caaaagcaaa aaagaattta
     1621 ccgtcacctg ggatgataac cataccagtg tatatccctt tgggttgttg cgcgcggcct
     1681 gtccatgcgc ctcctgccgt ggcggacacg aaaatatgaa atcagagcct gatgctgaag
     1741 tattcacccg ccaaatggaa gactctcaat caacgcggct ggccaatgtc gtcgcagtcg
     1801 gctcctacgc gttgaccatt gtctgggaag acggccatga ttatggaatt tacaactggc
     1861 attatttgcg agcattgtgt ccatgtgcgg aatgtcgggg gaaagcgaaa aagcagtaat
     1921 cagtgaaaaa gtaatcagga taataaagag aggcatcctt ttggaatgcc tctctttcat
     1981 ctttcatctt ttcccgtaca ctcccccact ttccacctac ctactttggc gtcggtgttg
     2041 gtgttggcgt acctgttcca ccttttggtg tgggtgtctt tcctggtgtg gcggtcttgg
     2101 ttgtgccgcc tttcggcgta ttcgtcggcg tgaccatggt tttcaaatgc aattctgcgt
     2161 cggcacgcat ttgctcggtc attacatcgg catccaatgc cagaagcgcc ttccaatctt
     2221 tgatggcctc tcttgcttca ccacgtttct cctgaaccag tgcatgccag tacagcatca
     2281 ccgcggtttc ttcatccgtc tcagccagcg acttgagtgc ttccgccttg aggaaggcgc
     2341 tgccgaattt ctcctgcaaa taataggctc tcaacaaacc aagattgatt tcaaaggaag
     2401 tatcgtctgc actggcggct ttttcaagat cttccacggc agcatccagg ttgttcagtt
     2461 caaggttggc gagcccttta tagaccagga acttccgcaa gccattcttg ttaatcgcct
     2521 cggctttttc aaaattggat gtagccgtct cgtaatcgcc catttcataa taggcctgtc
     2581 caagcagggc aaagacctga tcatcttccg tttcataaac agcatagaca tccaacgctt
     2641 caatggcgcg tgcatactcg ccgccttcaa tgtgcagttt ccctaacagc ttgtacactt
     2701 ccaggttggt gatatccaac gagtaggcag tttcagcagc ctccaatgca cccttcttat
     2761 catccatcgc aagataggca tcggcatagg ccatgtaaac gacgggcgaa ttgggcatca
     2821 gcttatccgc gcggtcgaga tcaacaatgg cggcttcggg gtcgttgttg atgatgttgt
     2881 atcgcgcccg ctccagatac acttcaccaa aatcaggatc caaatcaatg gcttgctcaa
     2941 aaagagcatc cacattggct ttgggattat ccaacaatct ggcacgcgcc aaccccaaat
     3001 aggcagggcc aaaatcttcg ttaatcttca aggcctcgtt gtagaaatcc agggcgctat
     3061 cggcatctcc cttgaaacga taggcgtctc cgatcaaata tggcacatcg gcggcttcag
     3121 gttccaacga agcaatgatt tgcatgttgg tgatgtattc atcccaattt tcctgagcat
     3181 atgcggcaag tgccagtcga tattgatcgc ctgactgtgc agcacgcggc gtattgacat
     3241 aaacggccgt cggcgtatag gtttggggca ggaacatcca caagggggtg ggaccgatca
     3301 cagccttggt tgggggcgct gttgctccaa acagggtcgg tgtggtggta aaagttggag
     3361 aaggaccagg tgtattcgtt tcagtgggac gtatatttga gcggcgcggc agaataaacc
     3421 caaaaataac cgcagaaacc aaccccgcac cgatcaacaa tacaaatgcc aaacgcgtaa
     3481 tggggctcct cgcaaaagcc ttgaagccct tttcctttgg cttttcattc gcaagtaaaa
     3541 gtttttcctc ccatgcccgt ggacggttca tggggaacgg ctgaatgctt tcgtcagggg
     3601 tcaatgctcc gagcaggatc aagccgcgtt tcgctgtacc attttcaggg tccagtttga
     3661 gcgctgtttg caggcagtaa atgcgctctt tggtggaatc catcgcggcg ctgagccaga
     3721 tccaataggt tgggttattc tgatccgctt tcaaaagaag cgtgatcaac tctttggcgc
     3781 gggctttgtc tccgcggcgc aaagcatcca cagcatcctg aaacatggtg tcatcgggtc
     3841 ggtcttcagc catgactgga ttctaattca tttcatggaa ttcttcgacc tgatatatat
     3901 aaacttgcaa ttttgtcttt cgccacagat cgcgcctcgc ccccatcttg gaacataaat
     3961 ggtcaaggaa atcttctgca tcagggattt gctcccatac ctgcggaaga aaagtagcgc
     4021 ggcgaaaccc atctttcaag atgacgccat ccacatgcgg gcgcaatttt acaggcagtt
     4081 cttcgcccga agaatattcc agcaaatgag gaacggtcaa ccgcgacacc tcgagcttga
     4141 ttctgtttaa ttcattttct ccaacgggtg gaaaacgcgg atcactcaaa gccgcagcaa
     4201 tggcatgttc acgcacatca tctaccaacg gttgataagc ctcgagagcg ccaatgcagc
     4261 cgcgcaattc accatgaatg gtaagtgtga cgaacgatgc accattttca cgcaaacgcg
     4321 gagagagaga ttcgttttcc aaaggcggaa gtttcattcc ccgcacagca tggtccattg
     4381 cttcacgtgc aaggcgcagc aaaatttgct tttccccatc ggtcaattgg tcgttcatgg
     4441 tagcctccca taaacaggcc cgaaaggtta tcaaaccttt cgggcctcaa tttatatttc
     4501 agacaaattc caatattttc ggttgtgata gattaacggc gggccatcgt ttgcttcctg
     4561 tgcagcaagt acttcagcaa tgaaaagcgt attcatgcct gcatcgaaag tctgcaccac
     4621 gcggcaatcc agccaggcca gaccacccgc gatcaagggt gaacctgtga ccatggtttc
     4681 cgtattcaaa ttggaaaatc gatcttcgat ccctgtcacc tgccctgaaa acaggtttga
     4741 aatatcagcc tgttctgcag aaaggatcgt caggccaaag gcaccagatt tggtcaccaa
     4801 ttcatgtgta cgggtacccg tccgcagaga cacggtgatc atggcaggct ccagcgaaat
     4861 ggacgtaaat gagtttacgg tcataccatg tttgtggcca tcatgtacgg cggtcaccac
     4921 agtcaccccc gccgaccagg cccgcatggc cgagcgtaac ttttcaggat caagagtcat
     4981 aagtgttgta tttttcatca tgtgaaattc atcataattg tgagatggga gagcgtcaag
     5041 tcaaaaatgc gattttagga caaggtcgtt ttttgttttc ttaccaacag gaacatcttt
     5101 agaaaattcc catctttcgc agatgatttg atgagtttca atccgaatac tgcgctcctc
     5161 tcaagttaaa ttcccattca taaacgcgtg gtagactcgc cccatgtcca aacgtattcg
     5221 aaatatcctc atcgggctgg cagggttgtt cttgttggct gttttggtgt accaaattcc
     5281 gtatgtcaat tcggttcttt cgtggcgcat cgaaaaattc atgatctata caaagaacgt
     5341 atttgacccc gtcggcccag tgccaactgc attgccagtc accccccacc ccattacgcc
     5401 aacctccatt gccacagcca ctgaaacgcc agccccagtc attgacctga cgccttccat
     5461 cacacccacc gcaacttttg cgcccctgcc cgcacaggca tccattgcgt caccgccttt
     5521 tgagcaacaa accccaaaca actgcggccc cgccactctc tcaatggcgt tacatctcta
     5581 tggctggaaa ggcaatcaag ccgatattgc caacgtcatc aaacccattt tacaagaccg
     5641 caatgtcaac cccgaagagc ttcgctatta cgtccgcaca caagcgggct ggctcaacat
     5701 ggaatatcgt gtgggcggca gcatcgaaac ccttaagcga ctgatcgccg caaattatcc
     5761 cgtcatcgtc gaaagtgtca cctcgctcga cccgaacgat gcactgggtc caaacgatga
     5821 cttgtgggct gcccactacc ttctgctcac aggctatgac gacaacaccc aaaccttcac
     5881 cattcaggat tcctatcacg gcgcagatct ttcactgccc tattctcaac tcgaaaagga
     5941 atggaagccg ttcaacaacc tgtacatcgt gatctacttt ccacaatttg aagatgagat
     6001 gaagagcctg ctcgcttcca actgggacgc aaacctgaat cggcaattgg cattatcagc
     6061 gtctgaaacc gataccgccc aaaaccccac cgatgcattt gcatggttca actatggcag
     6121 caaccttgtc tactttgatc gctatgaaga agccgcgcaa gcctacgaca aagcccgcga
     6181 gatcgggctg cccttgcgga tgttccgcta tcagttcggc cccttccttg cctatttcca
     6241 ttccaaccgc aatgaagacc tgctggtgct gaccgattat gcgcgcggtg tcactgaaat
     6301 gtctgaagaa gcctggttgt ggtatggata cggtctgtat cgcaacggcg attatgacgg
     6361 cgcgctcaag gcctggcaaa aagcagacca gatcaacccc aacttctacg aagatcaggc
     6421 caagaacgcc atcagtctaa ttcaataaat ccaaaaagca aattagcatt tcgcgcacat
     6481 gaatcacaga aaaccaccga tgaagaaaac cctcctgttt cttattcttt tgctcaccgc
     6541 ctgtcaaagc acccccacac ctgcgcccgc gcctgaggaa accgtcacgg ttgcgccatc
     6601 agaaacgcct gcgccgacag tggtttcatt cacagcaact cctgaggcca ctttcactcc
     6661 tgttccaact gccacgcccg aaactatcaa gatttcaacc gccgatggca tgacccaact
     6721 cctcatccct gctgggacgg tgaaaatggg cgggctggat gtactgcttg aaaatgatga
     6781 gatccctgca cacgatgtaa cgcttgatgc attttggatg gatcaagtgg aagtaaccaa
     6841 cggcatgtac aacctgtgtg tgcaggcggg cgtttgcagg ctgcccgtca aactcaattc
     6901 agataatcgc ggagattatt ttggaaatcc tgagtttcag gattatcctg taattcaggt
     6961 gacgtggttt gacgcaaata cctactgtca atgggcgggg cgcaggctgc ccaccgaagc
     7021 agaatgggaa cgtgccgcac gcggcgatga cctgcggact tatccctggg gcggtgaacc
     7081 tccgaatgaa ctgaactcga atgcaaataa catcgtcggc gatgtgaccc gtgtgggttc
     7141 gtatcctgcg ggggcaagtc cgtttggcgt gctggatatg gcgggcaatg tgtgggaatg
     7201 ggtggcggat tattacagtg gaagatatta cggagagtca ccagccataa acccactggg
     7261 gcccgagcat ggcgggttga attttctacg tgtgattcgc ggcgggtcat ttcaggataa
     7321 catgttcaat ctgcgcgttt ccaatcgtgg atatgagaca ggaccagacc catccgcttt
     7381 tccgggggag ccagaatatt acgggcgcag ttcggtgaag atcgggtttc ggtgtgcgaa
     7441 tgctgattaa agcagaatca aactacagtt aagagcgaag cggttctcca tcttcgttaa
     7501 tatttttatc tttacgctgc aagttatcta tgatattatc gcgaaacaat tcaacagccc
     7561 caatacggcg ctcttatcca gagaggcgga gggaacggcc ctgcgatgcc tcgacaacca
     7621 gtcagcgaaa gttgaatatg gtgccaaatc cgacagaaat ttctgggaga tgagagggta
     7681 gataagtttt atacgattgc cctttcacgt cgaaagggca attatatttt tcaaggagaa
     7741 acaaaaacga tgagcaatac ctttatgact tcgcccagcc tgatgtttac atcagagtcg
     7801 gtgacggaag gacaccccga taagatctgc gatcaggttt cggatgcggt gttggatgcg
     7861 tgcctcgaac aggatccgcg ttcacgtgtg gcttgcgaga ccgcagtgaa gacgggttat
     7921 gtaatgctgt tgggcgagat caccaccaat gcggttttca attacgatga actcgtgcgc
     7981 aaggtggtga atgatatcgg atacgactcg agcgagaagg gtttcgatgg aaattcctgc
     8041 ggcgtgctgg ttgcgatcgc aaaacaatca ggcgatattg ccatgggtgt gaatcaggcg
     8101 cttgagaccc gtgatggtca caagatgagc gatgccgaag tggattcgat cggcgcgggc
     8161 gatcagggca tgatgtttgg gtatgcctgt aacgaaacac caaccctgat gccgcttccc
     8221 atttatctgg cgcataaact cacccgccgc cagagcgagc ttcgcaagag cggcgcgatc
     8281 aagtggctgc gccccgatgc caagagccag gtgactgtgg aatattcgaa aggtcagccg
     8341 aagcgcattg acacggtgtt gatctccacc cagcactcgc ctgaggtttc gcagaatgaa
     8401 atcaccgaga tcatcactga agagttgatc atgaagactc tgcccgaaaa ccttgtggac
     8461 aaagatatca aggtctttgt gaacccgaca ggtcgttttg tggtgggcgg cccgatgggc
     8521 gatgcaggcg tgactggccg caagatcatt gtggatactt acggcggcat gggtcgtcat
     8581 ggcggcggcg cgttctccgg gaaagacccc acgaaggtgg atcgttccgc agcgtatgcg
     8641 gcgcgctatg tggccaagaa tgtagttgca gcagggctgg ctgaccgcgt ggaagtgcag
     8701 gtttcgtatg ccatcggtgt ggcacatccg ctttcggtga atgtggaaac cttcggcact
     8761 gccaggatcg cagatgacaa gatcgccgct ttgatcaacg agcattttga tctgcgccct
     8821 ggcgcgatca tccgtgacct cgacttgcgc cgtccgatct atcgcaagac tgccgcttat
     8881 ggtcactttg gccgcgatga tatcgaattc ccgtgggaaa agactgacaa agctgcggct
     8941 ttgaagaagg ctgcggggtt gtaagttagt gcttggtaat tggtaattga aaagagccgc
     9001 gagacttgag tccgcggctc tttttttaca taacgaagtg gattatggta atggcaagtt
     9061 cttggctttg acgatcag
//
LOCUS       DAOQJC010000147         9065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_4085_length_9065_cov_9.469034, whole genome shotgun sequence.
ACCESSION   DAOQJC010000147 DAOQJC010000000
VERSION     DAOQJC010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9065
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4085_length_9065_cov_9.469034"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..769
                     /locus_tag="PLE39_16645"
     CDS             158..769
                     /locus_tag="PLE39_16645"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE70015.1"
                     /translation="MSPRTYKASEQTKTNILQKAVELFNEHGTAGISMNSLAETLGMS
                     AGNLQYHYRNKEELLRALLELMFKEFDAIYPSSASTFTLDTLRSLMRLNFELSWKYRF
                     FFRELGALLRNDESLAIRFREIQEKRLQDQENIFKALMSSTQKKSSLSAEELRNVVLI
                     GWVLGNTWLSYMESMGQKINETALQQAVEVMVQHYKPYLKEIL"
     gene            771..1919
                     /locus_tag="PLE39_16650"
     CDS             771..1919
                     /locus_tag="PLE39_16650"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNE70016.1"
                     /translation="MKKKTAIIIGAGIGGLCTTLALRKIGIEATVYERSGILGNVGAG
                     LVVAANAIRSLRRIGLGDEAVAAGSKLHLTQIRASNGDILASTDMSSYESLFGAPTIA
                     IHRAALHKVLMDGLPASNIKLGAPCTRVEQDEEGVTAQFEDGSKARADFLVGADGVRS
                     IIRRQLFPSIELRYSGYTAWRGAVTSRDEVALGITSETWGRGNRFGIIRMDADRIYWF
                     ATANTKQGVTYAPDEQKKVLLDIFGSWHHPVKHLIESTPADVILHNDISDFAPMSQWS
                     VGRITLLGDAAHATTPNMGQGACMAIESADVLAQCLSKNDDIVSAFKEYETRRKPRTA
                     FVTDRSWQIGRMAQWENPLACAVRNFAVKTFSSHMMTKQLSKILGAES"
     assembly_gap    2077..2176
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2250..3482
                     /locus_tag="PLE39_16655"
     CDS             2250..3482
                     /locus_tag="PLE39_16655"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE70017.1"
                     /translation="MESKITVYGAYWCPDCRRSKKFLGENFIPFTWVDIEEDKEGEAF
                     VIAKNGGKRIIPTIVFEDGSFLVEPSNAELAKKLNLKTEAKKTYYDLIIIGGGPAGLT
                     ASIYASREGADTLLIERSGLGGQAGITVGLDNFPGFPEGISGQEFAERVTQQAKRFGV
                     EILQAQDVEKIMADDGYNEVYTSDGKHYHARAVLITSGASYRRLDIPGEDDFIGAGIH
                     FCATCDGPFYKGAKNIVVIGGGNSACEEGLHLTTFAEKVTLLVRGDKLTASQVAIDKI
                     NEPTSRVEVKFNVAVDSFEGENSKLKKIHFHDSVTGEKSEISPEAAFVFIGQKPNTEF
                     AKEALELDRYGFVLTGHDFSHAQSGKIHGERLAFETSMSGVFAAGDARHGSTKQVASA
                     VGEGAAAAISIREFLKRN"
     gene            3494..4468
                     /locus_tag="PLE39_16660"
     CDS             3494..4468
                     /locus_tag="PLE39_16660"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE70018.1"
                     /translation="MKYFITGATGFIGGYLARQLREAGHEVIAVVRNPAKAQTLSQLG
                     VTLHQGDVTEKESMRKPMSGVDGVFHVAGWYKVGVRDKSQAYAINVEGTRNVLELMKE
                     LNIPKGVYTSTLAVNSDTHGVEADENYHFTGKHISVYDQTKAEAHDIAVKMIKEGLPL
                     VIVQPGLVYGPEDTSSVRTSLIQYLTKKLPVMPKQTAFSWGYIEDIAHAHWLAMEKGK
                     VGENYNICGPTYKFTDALAIAKEITGVRLPMAVPAGMIRAMSAMMYVFDRFIPVPESY
                     TGEGLRIIAGVTYIGSNAKAKRELGYNPRPLKDGLEITLKHEMKLLGM"
     gene            4472..4996
                     /locus_tag="PLE39_16665"
     CDS             4472..4996
                     /locus_tag="PLE39_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70019.1"
                     /translation="MKTISLGRIFGLPIEILPLAFLGSFILWIGFTAIAFLGIKLPFV
                     ESLVLGLIATLLHWTFELIHGFGHAYAAKRTGYPMTGIALGTLAFFALTRYPMDEPEL
                     PPSIHIRRALGGPIINGLLSIVFFLALPLWPGDLFWIGIFALFENLFVYTLQVFVPLG
                     FNDGSTILRNLRRK"
     gene            complement(5084..5998)
                     /locus_tag="PLE39_16670"
     CDS             complement(5084..5998)
                     /locus_tag="PLE39_16670"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNE70020.1"
                     /translation="MKNLKRILLGLLAVLAILILFLPLSIFVDSLFDGNRLDALVNTT
                     IPGLNGGPDVRAYVAKPEGEGPFPTVIMIHEFYGLRESIVGKADFLAKEGYLVIAPDT
                     FRGSTTSWIPRAIYQVINTKPEDVNTDLDSVYAWLETQSDVDAGRIAILGFCYGGRTS
                     LVYSLHNSKLAATVIFYGSPETDPEVLKSLPGPVLGIFGGADQSISVEEVDQFDKALT
                     TANIQHEITVYDGQPHAFVENVEGIQAGGAQGEAWAQMLAFLEKNLKNVTAQQNETSA
                     VSYRAPFAWKYYAMLVYEHAFGSASHQH"
     gene            6106..7002
                     /locus_tag="PLE39_16675"
     CDS             6106..7002
                     /locus_tag="PLE39_16675"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE70021.1"
                     /translation="MKKIIITISTILFLAAAGLTAWNFDVVARSWRAAQEGRLSLLKL
                     RYELAAFDIGRPSPNIPLNTKVSTMDGMMQVYVGEGIFVMGSNDDPRSKEFPAHGVYL
                     DAFWMDKYEVSNAMYAKCVDAGVCLPPVLRLNPYYGKWAYRNLPVVYANWYAAETYCA
                     WAGRRLPTEAEWEKAARGTDERRYTWGEDRPNPRLANFEETLMGEMLPVFRYPLGASP
                     YGVLNMAGNVREWVADWYAKDYYANSPKVNPTGPESGFERSLRGGAYDANADSITTIN
                     RFRHEPDSAGLSRGFRCAENAK"
     gene            7012..8910
                     /locus_tag="PLE39_16680"
     CDS             7012..8910
                     /locus_tag="PLE39_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70022.1"
                     /translation="MKKLINKPSPAVWQVICLLFSLGLYTLIMVNRSPNLLRPISMAL
                     RTGVGLVIPITTLVLYAVFRVKDRPGDLLSMAATLSLFAMPLAGLWASGLSQSVTISG
                     LLPLTDASFYYQDSLRIIMGQDISHFSAMRPFFPGFLSFLMYLTDRNFMFSLAIVTAI
                     AAIGIHYTVREIQRTHGTEVAVFLLVILFLYFRHHSGTSMSETLGVPLGALGMALIWR
                     SLEKRSKILAIFGLFICAFALNVRPGTMFILPLILLWSAWVLKKPSEFIAVKFLFIGA
                     AVIAVSFFLNSQYVRLLAEPSGTAFSNFSWALYGLASGGNAYTYVFEQHPELRLIADP
                     EQSRTIYRMALELIVQSPQLFVQGALHNWGMFFSDSWYSAFSFLESETGIVHRITRWI
                     IYTLCVLGFIKWLRKPSDPHSGLVAVAAIGVLASVPFVPPTDAYRVRLYAASIVTFGL
                     LPAMGISLLLNLFKLKLPSKADSEIQQSNVSAIFSALLVILVLVGPLVVKANSQPPPA
                     LEFSCPTGSDKIAIRFDAGSSINILREKDLFLDWMPNFHQGAFSRSIHDLADNDLIAY
                     FDTLRPGTSLISTVDTLSYKAALITIPTHLLPKPGNYLGLCGHWATEPRLQYYSLFTA
                     EEATLIER"
     gene            8962..9051
                     /locus_tag="PLE39_16685"
     tRNA            8962..9051
                     /locus_tag="PLE39_16685"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8998..9000,aa:Ser,seq:gga)
BASE COUNT         2266 a         2290 c         2211 g         2198 t
ORIGIN      
        1 gaagccaatc gtttactggg tgctccgcaa gtgacgttac gagagtggtt agccagaagt
       61 tgagctcgat aaaacacctt cgggtgtttt tttgccactt gacaatttag taaatatata
      121 ctaaattagt aaacacatac taaattcagg aatcaccatg tccccgcgaa cctacaaagc
      181 atcggaacaa accaaaacca acatcctgca aaaagcagtg gaattgttca atgaacatgg
      241 cacggctggc atctcgatga attcacttgc tgaaacgctt ggcatgagtg cgggcaattt
      301 gcagtatcac taccgaaaca aggaagagct tttacgtgcg cttctcgaat tgatgttcaa
      361 agaattcgat gcgatctacc cgtcaagcgc ctcaactttc acactcgaca cattgcgcag
      421 cctgatgcgc ctcaactttg agctttcgtg gaaataccgc ttcttctttc gtgaactcgg
      481 cgccctactc cgcaatgatg agagtcttgc aatacgattc cgtgagattc aagaaaaacg
      541 tttacaagac caggaaaaca ttttcaaagc gttaatgagc agcacccaaa agaaaagttc
      601 attaagtgcg gaagaactcc gaaatgtggt gcttatcggt tgggtactgg gaaacacctg
      661 gctctcatat atggaaagca tgggtcagaa aattaacgaa actgccttac aacaagccgt
      721 ggaagtaatg gtgcagcatt acaagccata tttaaaggaa atcttataac gtgaaaaaga
      781 aaacagccat catcattggc gcaggcattg gcggactttg caccacactc gccttgagga
      841 agatcggtat cgaagccaca gtttatgaac gctcaggaat actcggtaac gtgggcgcag
      901 gtttggtcgt cgcggcaaat gcgatccgct ctttgcggcg catagggctg ggagatgaag
      961 cagtagcggc aggttcaaag ttgcatctga cacagatccg tgcaagcaac ggagatattc
     1021 tcgcatccac cgatatgagc agctacgagt cacttttcgg cgcgccgacc atcgccattc
     1081 atcgtgcggc attgcacaag gttttaatgg atggactgcc tgcatcgaat ataaagcttg
     1141 gcgcaccctg cacccgtgtg gaacaggatg aagaaggagt aaccgctcag ttcgaagatg
     1201 gaagcaaagc gcgcgcagat ttcctcgtcg gtgcagatgg agtccgctca atcatccgcc
     1261 gacaattgtt cccgtccatt gagttgcgct actctgggta cactgcctgg cgcggagccg
     1321 tgaccagccg cgatgaagtt gcgctgggca tcacctctga aacatgggga cgcggcaacc
     1381 gctttggaat catccgcatg gatgcggatc ggatttattg gtttgccacg gcaaatacaa
     1441 aacaaggcgt cacctacgcg cccgatgaac agaaaaaagt attgctcgat atttttggaa
     1501 gttggcatca tcccgttaaa cacctaatcg agtccacgcc tgcagatgtg attttgcaca
     1561 acgatatttc tgatttcgcg ccgatgtctc agtggagtgt ggggcgtatc acgttgctcg
     1621 gcgacgcagc gcatgccacc acgcccaaca tggggcaggg cgcatgcatg gcaattgaat
     1681 cggcggatgt gctggcgcaa tgtttatcaa aaaatgatga tattgtttcg gcgttcaaag
     1741 aatatgaaac ccgccgcaaa ccgcgcaccg cgttcgtcac ggatcgatcc tggcagatcg
     1801 ggcgcatggc acagtgggaa aatccgctgg cgtgtgcagt gcggaatttc gctgtcaaaa
     1861 ctttttcttc acacatgatg accaaacaat tatcgaaaat cctaggagca gaatcatgaa
     1921 aaaccttatt aaatttgaag agctctttat gttcgcattc agcatcttcc tgttctcaaa
     1981 gctgaatttc gcctggtggt ggtatcccgt tttgttgttc accccagacc tgagcatgat
     2041 cggctacatt gtcaatccac gcgttggttc gtgggcnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnncctc atcaactcat gaggtcacaa caaggctgcg ttataccaaa
     2221 aacaaaaatc aacagccaga ggagaaaaca tggaatcaaa gatcactgtc tacggagcgt
     2281 attggtgtcc cgattgccgc cgttcaaaaa agtttttggg cgaaaacttt attcctttca
     2341 cctgggtgga cattgaagag gacaaggaag gagaagcgtt tgtgatcgcc aaaaatggcg
     2401 gaaaacgcat cattccgacc attgtatttg aagacggttc cttcctggta gaaccatcca
     2461 acgcagaatt ggcaaagaaa cttaacttaa aaacagaagc aaaaaagacc tattatgatt
     2521 tgattatcat cggaggcgga cctgccggtt tgaccgcttc catctacgcc tcaagagaag
     2581 gcgccgatac cttgctgatc gagcgctcag ggttgggtgg acaggcaggc atcactgtgg
     2641 ggctggataa tttcccaggc ttccccgaag gcatcagcgg acaggaattc gcagagcgtg
     2701 tcacccagca agccaaacgc tttggcgtgg aaattttaca agcgcaggat gttgaaaaga
     2761 tcatggcaga tgatggctac aacgaagttt acacctccga cggaaagcat tatcacgccc
     2821 gcgctgtgtt gatcacctca ggtgcatcct atcgcaggtt ggacattccc ggtgaagacg
     2881 acttcatcgg cgcagggatt cacttttgtg ccacttgtga tggtccattt tacaaaggcg
     2941 caaaaaatat cgttgtcata ggcggcggaa attccgcctg tgaagaggga ttgcatctca
     3001 ctacatttgc agaaaaagtc actcttttgg tgcgcggtga caagctcaca gccagtcagg
     3061 tggccatcga caagataaac gagccgacct cacgagtgga agtaaaattc aatgtagctg
     3121 tcgattcgtt cgaaggcgaa aactccaaac taaagaaaat ccattttcac gactctgtca
     3181 caggtgagaa aagtgaaatc agcccggaag cagcatttgt attcatcggg caaaaaccca
     3241 acacagaatt tgcaaaagag gctttggagt tagacaggta tggatttgtt ctaacgggcc
     3301 atgatttttc tcatgcccaa tctggaaaga tacatggtga acgccttgct tttgaaacca
     3361 gtatgtctgg tgtttttgcg gcaggcgatg cgcgccacgg cagcaccaaa caggtcgctt
     3421 ctgctgttgg tgaaggtgcc gctgcagcca tttcgattcg cgaattcctg aaaagaaact
     3481 agaggaaaat aaaatgaaat atttcattac cggcgctaca ggctttatcg gcggatatct
     3541 ggctcgtcaa ttacgagaag cagggcatga agtgatcgcc gttgtgcgaa accctgccaa
     3601 ggcgcagact ctttcccagt tgggcgtcac cctccatcag ggagatgtga ccgaaaaaga
     3661 atccatgcgt aaaccgatga gcggtgtcga tggcgtcttc catgtggcgg gttggtacaa
     3721 ggtcggtgtg cgagataaaa gtcaggcata tgccatcaat gtggaaggca cacgcaatgt
     3781 attggaattg atgaaggaac tcaatatccc caaaggcgtt tacaccagca cgctcgcggt
     3841 caattccgat acgcacggcg tggaagctga tgaaaattat cacttcacag gaaaacacat
     3901 cagcgtgtat gaccagacca aggccgaggc acatgatata gccgtgaaaa tgatcaagga
     3961 aggtctgccg ctggtcattg tgcaaccagg gcttgtatat ggcccggaag acaccagcag
     4021 cgtgcgcact tcgctgatcc aatatctgac gaagaaatta ccggtcatgc ccaaacaaac
     4081 cgcgttttca tggggctata tcgaagacat tgcacacgcg cattggcttg cgatggagaa
     4141 aggaaaggtc ggcgagaatt acaacatctg cggaccgaca tataaattca ccgatgcgct
     4201 tgcgattgcc aaagaaatta caggggtacg attgccaatg gctgtgccag caggcatgat
     4261 ccgcgccatg tcggccatga tgtatgtgtt cgatagattc attcctgttc ccgagagcta
     4321 cacaggcgaa gggttgcgca tcattgcggg cgtcacatac attggcagca atgccaaagc
     4381 caagcgcgaa cttgggtaca atccgcgccc attgaaggac ggacttgaaa tcacattgaa
     4441 acatgaaatg aagttgcttg ggatgtaatt tatgaaaaca atttcacttg gcagaatatt
     4501 cggcctgccg atcgaaatcc tcccgctggc atttttaggc agttttattt tgtggatcgg
     4561 attcacagcc attgcttttt taggaatcaa actcccgttc gttgaatcgc ttgttctggg
     4621 attgatcgcc acgcttctgc actggacctt tgaactgatc cacggatttg gccatgccta
     4681 cgccgcaaaa cggaccggct acccgatgac aggcattgcc ctcggcaccc tcgccttttt
     4741 tgccctgacg cgttacccaa tggatgagcc tgaattgcca ccctcaattc acatccgccg
     4801 cgcattgggc gggccgatta tcaatggatt gttgagcatc gtctttttcc tggcacttcc
     4861 cctttggccc ggtgacctct tttggatcgg gatcttcgcc ctgttcgaga atttatttgt
     4921 ttacacgctg caggtctttg ttccactggg gttcaatgac ggcagtacca ttttgagaaa
     4981 tcttcgaagg aaataagaac gatttgctga atccccattt tcttccaagc tccagccgcc
     5041 gaggacggcg gctggagcag taatcaaata tctttttaca attctagtgt tggtggctgg
     5101 cactgccaaa agcgtgttcg taaaccaaca tggcgtaata tttccatgca aacggggcgc
     5161 gataagaaac cgctgaggtt tcattttgct gtgctgtcac gtttttcaag ttcttttcca
     5221 gaaaggccag catttgtgcc caagcctcgc cttgcgcacc gcctgcctga atcccttcca
     5281 cattttctac aaacgcatga ggctgaccat cgtacacggt gatctcatgc tggatattgg
     5341 cagtggtcag ggctttgtca aactggtcca cttcttccac ggaaatggac tggtcggcgc
     5401 cgccgaaaat tcccagcacc ggaccaggca aggacttcag aacttcgggg tcggtttcag
     5461 gggagccata gaaaatgaca gtggccgcga gtttgctgtt gtgcaggctg tacacaagtg
     5521 atgtgcgccc gccgtaacaa aaaccaagaa tggcaattcg gccagcatct acatctgatt
     5581 gggtttcgag ccacgcatag actgagtcaa ggtctgtgtt tacatcttca ggtttggtat
     5641 tgatgacctg atagatggca cgcggaatcc atgaggtcgt tgagcctcgg aaggtatctg
     5701 gcgcaatgac aaggtagcct tcttttgcaa gaaaatccgc tttgccaaca atggattcgc
     5761 gcaaaccgta gaattcatgg atcataatga cagtgggaaa tggcccctcc ccttctggct
     5821 tcgctacgta ggcacgtaca tcgggtccgc cattcaaccc tgggatcgtg gtgttgacaa
     5881 gggcatcgag acgattccca tcgaacaggg aatcgacaaa aatgctaagg ggcagaaaca
     5941 gaatcagaat cgccaaaaca gccaacagtc caagcaggat gcgttttaag ttcttcattg
     6001 aaatactcct aaaagattta atattcagat tttattctgg aaatcaatca aatcctctca
     6061 acaaatgccg ctttacaagt agagtaaaat caatccgtca tgtcaatgaa aaagataatt
     6121 atcaccatct ctactatttt gtttttggcc gctgcaggtc tgacggcctg gaattttgat
     6181 gttgttgcac gttcgtggcg ggctgcacag gaagggcggt tgagcctatt gaaactccgc
     6241 tatgagctgg ccgcgtttga tatcggcaga cccagcccca acatcccgct gaatacgaaa
     6301 gtatccacaa tggatggcat gatgcaggtc tatgtcggtg agggaatctt tgtcatgggc
     6361 agcaacgacg atccaaggtc aaaggaattt cctgcgcacg gtgtgtacct cgatgctttt
     6421 tggatggaca aatacgaagt gagcaatgca atgtacgcga aatgtgtgga tgctggtgta
     6481 tgcctgccgc ccgtgttgag gctcaatcca tattatggca agtgggcgta tcgcaatctg
     6541 cctgtggtgt atgccaactg gtacgccgct gaaacgtatt gtgcctgggc aggcaggcgg
     6601 ctacccacag aagcggagtg ggaaaaagcc gcgcgcggca ccgatgaacg ccgctacacc
     6661 tggggtgaag accgtcccaa tccgcgcctc gcaaattttg aagaaaccct gatgggcgag
     6721 atgctccctg tgtttcgcta tccattggga gccagccctt atggggtgtt gaacatggcg
     6781 gggaatgtcc gcgaatgggt ggcagattgg tacgccaaag actattatgc caactcgcca
     6841 aaggtcaacc ccaccgggcc tgaaagcggc ttcgaacgtt ccctgcgtgg cggtgcctac
     6901 gatgccaatg cagatagcat caccaccatt aatcgcttcc gtcatgagcc cgactctgct
     6961 ggattgagcc gcggctttcg atgcgccgaa aacgcaaaat aaaatcattc gatgaaaaaa
     7021 cttatcaata agccatcccc tgccgtttgg caggtcattt gccttctttt ttcgctcggc
     7081 ttgtacacac tgatcatggt caaccgttca cccaacctgc tgcgaccgat cagcatggca
     7141 ttacgcactg gtgtcgggct ggtgattccc atcaccactt tggtcttgta tgctgtcttt
     7201 cgtgtcaaag accgccccgg cgatctactc agcatggcag ccacactctc cctgtttgca
     7261 atgccactgg ctgggctgtg ggcctcgggc ctgtcacaat ctgtcaccat tagcggattg
     7321 cttccattga ccgatgcgtc cttttattat caagacagtc tgcgcatcat catgggacag
     7381 gatatctctc acttctccgc catgcgccca ttcttccctg gatttctatc ctttttgatg
     7441 tacctgacag atcgaaattt catgttcagc ctggccatcg tcacagccat tgctgcaatt
     7501 ggaattcact acacggtccg cgagatccag cgcacacatg gcactgaagt ggcggtattc
     7561 ctgctggtga tcctttttct ttatttccgc catcattcag gtacaagcat gtctgaaacg
     7621 ctcggcgttc cgctcggtgc ccttggcatg gcactgatct ggcgcagtct ggaaaaacgc
     7681 tccaaaatcc tggccatctt cggcttgttc atctgcgcct ttgccttgaa cgttcgtccc
     7741 ggcacaatgt tcattcttcc gctcatattg ctttggtcgg cctgggttct caaaaagccc
     7801 agtgaattca ttgccgtcaa attccttttc attggggcgg ctgtcattgc ggtgagtttc
     7861 tttttaaatt cccaatacgt gcgcctgctg gcagaaccat cgggaaccgc cttttccaat
     7921 ttttcctggg cattgtatgg gctggcctca ggaggcaatg cctacaccta cgttttcgag
     7981 cagcatcccg aactgcgttt gatcgcagac cccgagcaat cccgcaccat ctaccgcatg
     8041 gcattggaac tgatcgtgca aagtccgcaa ttgttcgtgc agggcgcact acacaactgg
     8101 ggtatgttct tctctgatag ctggtatagt gcgttctctt ttcttgaaag cgaaacaggc
     8161 attgtgcatc gcatcacccg ctggatcatt tacaccttgt gtgtgttggg atttatcaaa
     8221 tggcttcgca aaccctccga cccacacagc ggacttgtgg ctgtggctgc gattggcgta
     8281 ctggcgtcag ttccattcgt cccacccaca gatgcctacc gcgtacgact gtacgccgcc
     8341 tccatcgtga cctttggcct gctcccagca atgggcattt ccctcctcct caacctgttc
     8401 aaactaaaat tgccctccaa agcggattct gaaattcagc agtcaaatgt ttccgccatc
     8461 ttcagcgccc tgctcgtcat ccttgtgctg gtcgggcctt tggtggtcaa agccaattct
     8521 caaccaccac ccgcactgga attctcctgc cccacaggca gtgacaagat cgcaattcgt
     8581 ttcgatgcag gtagttccat taacatcttg cgcgagaaag atctgttcct cgattggatg
     8641 cccaattttc atcagggtgc tttcagccgc agcatccacg acctggcaga taatgacctg
     8701 atcgcatatt tcgacacact ccgccctggc acatcattga tttccacggt tgataccctt
     8761 tcctataaag cagcactaat cacaattccc acccacctct tgcccaagcc cggaaactac
     8821 ctcgggctct gtggtcactg ggccacggaa ccacgcctgc aatactacag cctgttcacc
     8881 gctgaagaag ccaccctgat cgaaagataa ttcaagtatt gccatacaaa taacacatgg
     8941 tataatctgc ccgctgaatt tggagaggtc gcgtagtctg gcttagcgcg cacgcttgga
     9001 aagcgtgtaa cccacaaagg ttcgcgggtt cgaatcccgc cctctccgcc agaaaaaccg
     9061 ctttg
//
LOCUS       DAOQJC010000148         8939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_4166_length_8939_cov_10.536920, whole genome shotgun sequence.
ACCESSION   DAOQJC010000148 DAOQJC010000000
VERSION     DAOQJC010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8939
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4166_length_8939_cov_10.536920"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="PLE39_16690"
     CDS             <1..1051
                     /locus_tag="PLE39_16690"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNE70023.1"
                     /translation="MKTTHPLLDLARKSGNPVITGNQVTFVWKGKSAPCLITDIHEWE
                     DHPQKLTRTAPDLWTCSLDLHRDAYLEYAFYDPRTKKRIKDPLNKKTVYNGIGQYNHF
                     FYMTAASPTIYTAKRKEIPVGRITRHLVDASLMHEGGKRELHLYHPPVHEPVPLLLVY
                     DGTDYLKRAMLNNIVDNLINEKRIRPIAMAFLQNGDDHRSLEYACSDATLTALDQSIL
                     PFASQKLNLLSPKQTRGAFGVLGASFGGLMSMYTGLRMPDIFGKVICQSGVFELNSRD
                     FVAVDLIRAKQSREIAIWMDIGRFDSLLEDNRRLQPLLRENGYNVTYREANAGHNFTF
                     WRNEIPMALEAMFPA"
     gene            1048..1506
                     /locus_tag="PLE39_16695"
     CDS             1048..1506
                     /locus_tag="PLE39_16695"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HNE70024.1"
                     /translation="MILATLCYVKKDGHTLMIHRNKRPNDIHEGKWNGLGGKFEKGES
                     PEECVIREVWEESGLTLRTPRLHGTLTFANFKGNHWYVFVFTAHEFEGKLLQDVPEGS
                     LEWVPDEKILDLPLWDSDQVFLPWIEQGRFFSAKFNYDGERFINHAVAFY"
     gene            1672..2454
                     /locus_tag="PLE39_16700"
     CDS             1672..2454
                     /locus_tag="PLE39_16700"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE70025.1"
                     /translation="MKLETITRVPENQTHQTPLLFIHGMWHGAWCWDEFFLPFFAGHG
                     FHVTALSLRGHAGSEGNIRGSKIADYIHDVEQIAKTLPTPPALIGHSMGGFLTQKYLE
                     RNTAPAAVLLASNPYFGLWPTFLRMSAQRPLTLLKVLSQLRLYPVVENSEAARWTLFS
                     KDMPEEQVLKYHKKMNDESFRMFLDLLGLNLAHPQKIKTPLLILGAEFDNAITAGQVH
                     ATARGYGLQAEIFPNMAHDMMVEAGWRSVAERILNWLNEKGI"
     gene            2457..4424
                     /locus_tag="PLE39_16705"
     CDS             2457..4424
                     /locus_tag="PLE39_16705"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE70026.1"
                     /translation="MKKTFIGIAVLVITTLACSTLMPAQSAAEPTVGATDVPAPGSGV
                     GLSGLDGFPCPDSDFTCVTLTVPLNHFDPSDSRTLEVVFGVLPATGERKGMFVTATGG
                     PGTAGLMSADGYTAVMDPAIPEHYDIVFFDQRGVGQSGGLQCTNAAAKYFLADFSTGT
                     PEEEQALLQTARSFANDCVTEAGNPDFLPYLGTAQAVEDLELFRQAMGDEKIWLYGES
                     YGTQYAQTYAAAHADHLAGLILDGTVDLTLTGLEYYTQQAAAFNSTLTLTLEACNADD
                     ICAESMGGGDAIAAYNKLSQRLKQGSIEFNFPLPSGGIANRAFTFSDLENSAASYMYS
                     ETLRMIFLRALAAYSRNEDIVPMARILYASLALDPETLIPKTDPSYSDALFYAVECQD
                     YSYFSGTPEERGTALMQAGNAVEQSLENFESIFYGDVPCLFWPNTPTENTRPAPLVAD
                     GVPTLVLGATADPATPVSNGIAVFRRLADGYLVTQNGGPHVTYGVGSACVDAIVTEFL
                     VNGQPPAERENTCEGVVADEFISLLPLDAKEFIDPLDALTMIEDEIYLLPEYYYWDYI
                     TPTSIGCPFGGVMTFEAADAGDSFSFDNCSFTNGFSLSGSGFNNLDDGTFAFDVTVSG
                     LKAGTLTYLHDSEGGRNVRGEYGGEAIDISR"
     gene            complement(4449..4946)
                     /locus_tag="PLE39_16710"
     CDS             complement(4449..4946)
                     /locus_tag="PLE39_16710"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HNE70027.1"
                     /translation="MKDHILAALQEQFHQLEALLESLSEEQMTAPNFDEDWSVKDIVN
                     HLWGWQQISIARMKAGLLDREPEFPAWLVSFPGNWDENASQTNAWIYKNFHDQPWAEV
                     NKKWREGFLQLLDLGGRVSERYLLDGDRYVWLKGYSLAFILVASYEHHQEHLEKLVIW
                     LKDQQ"
     gene            complement(4977..5741)
                     /locus_tag="PLE39_16715"
     CDS             complement(4977..5741)
                     /locus_tag="PLE39_16715"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HNE70028.1"
                     /translation="MNLRNAFSQLGLSKQLVIAHASLRAFGSVEASAVLDALLETTRG
                     VIMPTFTYKTMLNPEVGPPHNGITYGSEADLNKMAEPYRPDMPVDKLMGILPEVLRNH
                     PKARRSSHPIQSFAGINADAIVNAQTIYEPLAPIAALAERDGWVLLLGVDHRVNTSIH
                     YAEKLSGRMQFIRWALTPGRVVECPGFPGDSEGFNAIAPAVEKFTRRVEVGNALVQAV
                     PLRNLIHAVMDAIRDNPLALLCGRPDCERCEAVRWG"
     gene            complement(5793..6566)
                     /locus_tag="PLE39_16720"
     CDS             complement(5793..6566)
                     /locus_tag="PLE39_16720"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNE70029.1"
                     /translation="MAYTTILTETRGRVGIVTLNRPQAMNAFNSTMLTELFDALEAFD
                     TDPAVGAMVVTGNEKVFAAGADIKEMAEASPFEMITQGRVEKWDRIRAIKKVVIAAVS
                     GWALGGGCEFAISCDMIVASESAKFGQPEITIGVIPGGGGTQRLARLLGKQLAMEMVI
                     NNRTLTAAEALQFGLANRVVPVEGYLDSAIAFAEEIASRAPMAVRMAKDAVNAAFETT
                     LTEGLRAEKRNFYPLFSTEDQKEGMKAFIEKRKANWKGK"
     gene            6662..7060
                     /locus_tag="PLE39_16725"
     CDS             6662..7060
                     /locus_tag="PLE39_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70030.1"
                     /translation="MQILLMIHSYVRWLIVLVAIAAVIKFALGWLRGGQFKGMDRGLA
                     SGFSGLIDLQATLGLILMLWSGFSGVGFPMFRIEHATTMVIAAVVAHLPARWKTAADK
                     TRFRNTLFCVLGALLLIYVGVMRLPGGWSR"
     gene            7171..7476
                     /locus_tag="PLE39_16730"
     CDS             7171..7476
                     /locus_tag="PLE39_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNE70031.1"
                     /translation="MENKLYVGNLPYATTEDELKAHFSQAGTVTSVALIKDRATGRAK
                     GFGFVEMSTADEAQKAISMFNGQNFMGRSITVNVARPPENRPRNFGDRNRGGGQNRN"
     gene            7546..8127
                     /locus_tag="PLE39_16735"
     CDS             7546..8127
                     /locus_tag="PLE39_16735"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HNE70032.1"
                     /translation="MKRIILLGPILFAIVVSTLTLVKYEFLRGLGWHPIHDPTFDWPS
                     GLALGPYGWIMTTTFIISGSLMTLFAARLFLDLEPVRTSQIGCALLAFSGLALAALAF
                     TTDPTLRTTPATWHGRLHDLSFVLLGLTLFPAMIVLGFAFRKSERWKPYAFYTWQTIA
                     LAAPAFAIKGAAFYVFLFAILLWNEIIALKLTD"
     gene            8216..8773
                     /locus_tag="PLE39_16740"
     CDS             8216..8773
                     /locus_tag="PLE39_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70033.1"
                     /translation="MRIARAIKTDQETIKRFLDVLGGGSVALGNSRLAQVSFFVLGHS
                     FIQEFIEGIFRKKEEYLINALEDGGFPTEGGPIGSLKADQKKCLEAAEHLLHASQQWQ
                     DGDEIARSEVGWAVSEFNNSCRQRLDRMKNLIIPLLEQTFSLEEDQKMAEEITNMAFE
                     NLPKDTPDKYIKVIEKLEEQLSDWK"
BASE COUNT         2129 a         2591 c         2187 g         2032 t
ORIGIN      
        1 catgaaaacc acccatcccc ttctcgacct tgcccgtaaa agcggaaacc ctgtcatcac
       61 aggaaatcag gtcacctttg tatggaaagg gaaatccgcg ccgtgtttga tcactgacat
      121 ccacgaatgg gaggatcatc cgcaaaagct gacccgcacc gcccccgacc tgtggacctg
      181 ctcactcgac ctgcatcgag atgcctacct cgaatacgcc ttttacgacc cgcgcacaaa
      241 aaaacgcatc aaagacccat tgaataaaaa aacggtctac aacggcatcg ggcaatacaa
      301 ccacttcttt tacatgaccg ccgcaagccc caccatctac accgccaaac gcaaagagat
      361 ccccgtcggc aggatcaccc gtcaccttgt ggatgcatcg ttgatgcacg aaggcggcaa
      421 acgcgaactg cacctctacc acccacccgt ccacgaacct gtgccgctcc tgctggtgta
      481 cgacggcacc gactacctca aacgcgccat gctcaacaac atcgtggata acctcatcaa
      541 cgaaaaacgc atccgcccga tcgcgatggc cttcctgcaa aatggagatg accaccgcag
      601 cctggaatac gcctgttcag atgcaaccct gaccgccctc gatcaatcca tcctgccatt
      661 tgccagccag aaattgaatc tactctcccc gaaacagacc cgcggcgcat ttggcgtatt
      721 gggcgcatcc ttcggcggat taatgtccat gtacacgggg ctgcgcatgc ccgatatctt
      781 tgggaaggtc atctgtcagt caggagtctt cgaattaaac agccgcgact tcgtcgccgt
      841 ggacctgatc cgcgcgaagc aatcccgcga gatcgcgatc tggatggata ttggccgctt
      901 cgacagcctg ctcgaagata accgccgact tcaaccgctc ctgagagaga acggctacaa
      961 cgtcacctac cgcgaagcga acgcaggcca caacttcacc ttctggcgga atgaaattcc
     1021 aatggcattg gaggcaatgt tccctgcatg atcctcgcaa ccttatgcta cgtcaaaaaa
     1081 gatgggcaca ccctgatgat ccatcgcaac aaacgcccca acgacatcca cgaaggcaag
     1141 tggaatggac tcggcggcaa atttgaaaaa ggcgaatccc ccgaagaatg tgtcattcgc
     1201 gaagtgtggg aagaatcggg gctgaccctc cgcacaccgc gcctgcatgg caccctcacc
     1261 tttgccaatt tcaaaggcaa tcactggtac gtctttgttt tcacagcgca tgaatttgaa
     1321 ggcaaacttc tgcaggatgt acccgaaggc agcctggaat gggtccccga cgaaaagatc
     1381 ctcgacctcc cgctttggga tagcgaccag gtcttcctgc cgtggatcga acagggacgt
     1441 ttcttctccg ccaaattcaa ctacgacggc gaacgcttca tcaaccacgc tgtggcattt
     1501 tattaactga tgttacgcag gactgcaaga tttatcctgc cattcgtttg ttttcccaac
     1561 ttgcaggcgc aaagtgaatt ttgcggcaca cccccaaccc catcccattc aaactgatac
     1621 aatggaagca tacattcgac ggacgattgg ccatgtaaga ggagagcagc catgaaactc
     1681 gaaaccatca ctcgtgttcc cgaaaatcaa acccatcaaa cgcccctgct ttttatccac
     1741 ggcatgtggc atggcgcctg gtgttgggat gaattcttcc tgcccttctt tgcagggcat
     1801 ggatttcatg tcacagcact cagcctgcgc ggacatgcag gcagtgaagg gaatatccgt
     1861 ggaagcaaaa ttgcggatta catccatgat gtggagcaga tcgcaaaaac gcttccgact
     1921 ccccctgccc tgatcggcca ttcgatgggc ggatttctca cccagaaata tctcgaaaga
     1981 aatacagcgc ctgccgccgt gctgctggca tcgaatccat actttggatt gtggccaacc
     2041 tttctgcgca tgtccgcaca gcgcccgttg accttgctga aggtactctc gcaactgcgt
     2101 ttgtatccag tggtggaaaa ttccgaagcc gcccgctgga ccctgttttc aaaagacatg
     2161 cccgaagaac aggttttgaa atatcacaaa aagatgaacg atgaatcttt tcgcatgttt
     2221 cttgacctgc tcgggctgaa tctggctcat cctcaaaaaa tcaaaacgcc cctgctcatc
     2281 ctcggtgcgg aatttgacaa cgccatcacc gcgggtcagg tccatgccac ggcgcgggga
     2341 tacgggctgc aggcagagat cttcccgaac atggcacacg acatgatggt ggaggcaggt
     2401 tggagatcgg tagccgaaag aatcctgaac tggttgaacg aaaaaggaat atgaccatga
     2461 aaaaaacatt tatcggtatt gctgtgctgg tcatcaccac gctggcctgt tccacgctga
     2521 tgcccgccca aagtgccgca gaaccaaccg tcggcgcaac ggatgttcct gcgccaggat
     2581 caggcgtcgg cttaagtggg ctcgatgggt ttccttgtcc agatagtgat ttcacctgcg
     2641 ttacattaac cgtgccgctg aatcatttcg acccatccga ttcgcgcacg ctggaggttg
     2701 tctttggtgt tttgcctgca acgggtgaac gcaaaggcat gttcgtcact gccactggcg
     2761 ggcctggcac ggcaggtttg atgagcgcag atggatacac cgctgtgatg gaccccgcca
     2821 ttcccgagca ttacgacatt gttttctttg atcagcgtgg cgtggggcaa tcgggcgggt
     2881 tgcaatgcac aaatgcggcc gcaaaatatt tcctggccga tttttccaca ggcacacccg
     2941 aagaggagca ggcgcttttg cagacagcac gcagctttgc aaatgactgt gtcaccgaag
     3001 cgggcaaccc cgattttctt ccataccttg gcaccgctca agccgtggaa gatctggagc
     3061 ttttcaggca ggcgatgggc gatgaaaaga tctggttgta tggcgaaagt tatggcacgc
     3121 aatatgcgca gacctacgcc gcggcacacg ccgaccatct ggcggggttg atccttgatg
     3181 gcacggtgga tctcacgctg acagggttgg agtattacac ccaacaagcg gcagccttca
     3241 acagcacgct gaccctgacc ctcgaagcct gcaacgccga tgatatctgc gccgagtcga
     3301 tgggcggcgg tgatgccatt gcagcctaca acaaactttc acaacgattg aaacaaggca
     3361 gcattgaatt caacttcccc ctgccttcgg gcggcattgc aaaccgcgcc ttcacttttt
     3421 cagatcttga aaattcagca gccagctaca tgtattccga aacattgcgc atgatcttcc
     3481 tgcgcgcgct ggcggcgtac tcacgcaatg aagatattgt gcccatggcg cgcatcctgt
     3541 atgcatcgct ggcgctcgac ccagaaacac tcatccccaa aaccgaccct tcctattcag
     3601 atgcgttgtt ttatgcagtg gaatgtcagg attacagtta tttcagcggc acacccgaag
     3661 aacgcggcac ggctttgatg caggcgggaa atgcggttga acaatcgctg gaaaatttcg
     3721 aatcgatctt ttatggggat gtgccctgtc ttttctggcc aaacacaccc accgaaaaca
     3781 cccgccccgc gccgcttgtg gctgatggtg tacccacatt ggtccttggc gcaaccgccg
     3841 accccgccac accggtctcg aatggcattg cggtcttccg ccgccttgct gatggctacc
     3901 ttgtgacaca aaatggaggg ccgcacgtca cctatggtgt tggctcggcc tgcgtggatg
     3961 cgatcgtgac ggagtttctg gtgaacggcc aaccgcccgc agagcgcgaa aacacctgcg
     4021 agggtgtggt ggccgatgaa ttcatttcat tactgccgtt ggatgcaaaa gagtttattg
     4081 acccgctcga tgcactgacc atgatcgaag atgagatcta tcttcttcct gaatactatt
     4141 actgggatta catcacaccg acctcgatcg gatgtccatt tggcggcgtg atgacctttg
     4201 aagccgcgga tgcaggcgat tctttctcct tcgacaactg ctccttcaca aatggctttt
     4261 ccctcagcgg cagtggattc aataacctgg acgacggcac cttcgctttc gatgtaaccg
     4321 tcagcggatt gaaagcgggc acactcacct acctccacga ttcagaaggc ggacgcaacg
     4381 tccgcgggga gtatggcggc gaggccattg atatttcaag gtagaggctg cctgcctgta
     4441 cagcgggtct attgctgatc tttcagccag atcaccaatt tttcaagatg ctcctggtgg
     4501 tgttcgtaag aagccaccag gatgaacgca agcgaatatc ccttcaacca tacatatcta
     4561 tccccatcga gcaaatatct ttctgaaacc ctcccgccca gatcaaggag ttgaagaaaa
     4621 ccttccctcc attttttatt cacctccgcc cacggttggt cgtgaaagtt cttgtagatc
     4681 caggcatttg tctggcttgc gttttcatcc caattgcccg ggaaacttac aagccaggct
     4741 gggaattcag gctcacgatc gagcaagcct gctttcatcc ttgcaattga aatctgctgc
     4801 cagccccaca agtgattaac gatatctttg acagaccagt cctcatcgaa atttggggcg
     4861 gtcatttgtt cttcgctcaa cgattcaagc aatgcctcca actgatggaa ctgttcctgc
     4921 aacgccgcca ggatatgatc tttcatgctc atgatgtgcc tcattgggtc aaaccatcat
     4981 ccccagcgga cggcttcaca gcgctcgcaa tcgggcctgc cgcacaacaa agccagcgga
     5041 ttatccctga tggcatccat cacggcgtga atcagattcc gcaggggtac ggcctgcaca
     5101 agcgcattgc caacttccac acggcgggtg aatttttcca cagcgggtgc aatggcattg
     5161 aatccctctg aatcaccggg aaagccgggg cattccacca ccctgcccgg ggtcagcgcc
     5221 cagcggataa actgcattcg gcccgagagc ttctcagcat aatggatgct cgtattcacc
     5281 cgatgatcca ctccaagtaa cagcacccag ccatcccgtt cggccagagc cgcaatgggc
     5341 gccagcggct cataaatggt ctgcgcgttg acgatcgcat ctgcattgat ccccgcaaaa
     5401 gactggatcg ggtgtgaaga acgcctcgcc ttcggatggt ttcgcaacac ctcaggcagg
     5461 atgcccatca acttatccac gggcatatca gggcggtaag gttcggccat tttgttcaaa
     5521 tcggcttcac tgccataggt gattccgttg tgcggcggcc ccacctccgg attcaacatg
     5581 gttttgtagg tgaaggtcgg catgatcact ccgcgcgtgg tttcaagcag ggcatcgagc
     5641 acagccgagg cctccaccga accaaaggcg cgtagtgagg catgtgcaat gacaagttgt
     5701 ttgctcaggc ccaattgtga aaaagcattt cgcaggttca ttcgtttgac aatcagatgg
     5761 ttggatggtt aagggttcaa acagcgaaca ggctacttcc ctttccagtt cgccttgcgt
     5821 ttttcaatga aggctttcat gccttccttt tgatcttccg ttgaaaacag cggatagaag
     5881 ttgcgtttct cagcccgcag cccttcggtc agtgtggtct cgaaagcggc gttgacggca
     5941 tccttcgcca tgcgcaccgc catcggtgcg cgcgaagcga tctcttctgc aaaagcaatg
     6001 gcagaatcga gatagccttc cacaggcaca acccgattcg cgagcccgaa ctgcagggct
     6061 tcggcggcgg tcagtgtgcg gttgttgatg accatttcca ttgcaagttg ttttccgagc
     6121 aaacgcgcca gccgctgggt tccgcccccg ccgggaatca caccgatggt gatctcaggc
     6181 tgtccaaatt tcgctgattc agaagcgacg atcatatcgc aggagatcgc gaattcgcac
     6241 ccgccgccca gcgcccaccc cgaaaccgcc gcaatgacca cctttttgat cgcgcgaata
     6301 cggtcccatt tttcaacgcg accctgtgtg atcatttcaa aaggcgaggc ttctgccatt
     6361 tccttgatat ctgcgcccgc ggcaaagact ttctcgttgc cggtcaccac catcgcaccg
     6421 acggcggggt ctgtatcaaa ggcttccagc gcgtcgaaca actcggtcaa catggtggaa
     6481 ttgaaagcgt tcatcgcctg cgggcgattc agggtgacaa tgcccacccg tccgcgggtt
     6541 tctgtcagaa tggttgtgta tgccatgagt cctccaagtg gattgggcat ttccatgccc
     6601 atgcagtaaa ttataaggga tgaaaggcct tgttgtataa tcgcccactg gagaacaaaa
     6661 catgcaaatc ttattaatga tccattctta tgtccgttgg ttgatcgtcc ttgttgccat
     6721 tgcggctgtc atcaaatttg cactgggctg gctgcgcggc ggccaattca aaggcatgga
     6781 ccgcggcctt gcctctggtt tcagcggctt gatcgacctg caggccacac tcggcctcat
     6841 cctcatgtta tggtctggtt ttagcggtgt cggcttcccc atgttccgaa ttgagcatgc
     6901 caccaccatg gtcattgctg cggtagtggc gcatctgcct gcccgctgga aaaccgccgc
     6961 agacaagacc cgcttccgca acaccctgtt ctgcgtcctc ggcgcattgc tgctgatcta
     7021 tgtcggggtg atgcgactgc ctggcgggtg gagcaggtaa cggctgcgtc tgatcggtct
     7081 tttttccaaa aaattattct cagtttatat taatcatgat aaactttgtc atgtcgttca
     7141 ggctggaacg ttgacacact tggagaagag atggaaaaca agttatatgt aggcaacctg
     7201 ccctacgcca ccactgaaga tgaactcaaa gctcacttca gtcaggccgg aaccgttact
     7261 tctgttgcgt tgatcaaaga tcgtgccact ggccgcgcca agggtttcgg ctttgtcgaa
     7321 atgtcaacag ccgacgaagc gcaaaaggcc atcagtatgt tcaatggcca gaactttatg
     7381 gggcgctcca tcacggttaa tgtcgcccgc ccgcctgaaa accgtccgcg taatttcggt
     7441 gatcgaaacc gcggcggtgg acaaaaccgc aactagaaaa atctccagcc ctgaacctgc
     7501 atgaaatcaa cagccccgaa tgaacggggc tgttatcatt aaggcatgaa acgcatcatc
     7561 cttctcggcc ccatcctctt tgccatcgtt gtttccacgc tcacactcgt caaatacgaa
     7621 ttcttgcgcg gcctcggctg gcatcccatc cacgacccca ccttcgattg gccaagcggt
     7681 ctcgcacttg ggccctacgg atggatcatg accacgacct tcatcatcag cgggtcgctc
     7741 atgaccctct tcgccgcccg tctctttctg gaccttgaac ctgtccgcac ctctcaaatt
     7801 ggatgcgccc ttctggcatt ctctggttta gcccttgccg ccctcgcatt caccaccgac
     7861 ccaaccctgc gcaccacacc cgccacctgg catggacgtt tgcacgacct gtcttttgtg
     7921 ctgctgggcc tcacgctctt ccccgccatg atcgtgcttg ggtttgcctt ccgcaaaagc
     7981 gaacgatgga aaccctatgc cttctatacc tggcagacca ttgcgctggc cgctcccgcc
     8041 ttcgccatca aaggcgcggc attttatgtt ttcctttttg caattctgct ttggaatgaa
     8101 atcatagcct tgaaattgac cgattagaag taggcaggcc gaaaatttcc gctctgtttt
     8161 acggcatggt atactccaca aaacattaca tcccaaaaaa gaatcaggga aagatatgcg
     8221 aatcgcaaga gccatcaaaa ccgaccagga aaccatcaaa cgtttcctcg atgtactggg
     8281 cgggggatcc gttgcccttg gtaacagcag actggcccag gtcagttttt tcgtgcttgg
     8341 ccacagtttc attcaggaat tcatcgaagg tatctttcga aaaaaggaag aatacctcat
     8401 caacgcgctg gaagatggcg gcttccccac cgagggcggt cccattggct cactaaaagc
     8461 tgaccagaaa aaatgccttg aagcggccga gcaccttctg cacgcatcac agcaatggca
     8521 ggatggcgac gaaatagcgc gcagcgaagt ggggtgggcg gtcagcgaat tcaacaattc
     8581 gtgccgtcaa cgccttgacc gcatgaagaa tctgatcatc ccactgctgg aacaaacctt
     8641 ctcccttgaa gaagatcaaa aaatggctga agagatcacc aacatggcct tcgaaaatct
     8701 tcccaaagac acgccagaca agtacattaa ggtcattgag aaactcgaag agcagttgag
     8761 cgattggaag tagcagtaaa taagtcatca ctcaaaacag gtcagaaact ctgacctgtt
     8821 ttgtctttaa ttgcctaaac tctggattgc cagcaaatta tatataatgt acaatccgcg
     8881 ccaatcaagg aatcattccc ccatgctaaa tctcaacctc aaaaccaccc ccttccacg
//
LOCUS       DAOQJC010000149         8920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_4179_length_8920_cov_6.852228, whole genome shotgun sequence.
ACCESSION   DAOQJC010000149 DAOQJC010000000
VERSION     DAOQJC010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4179_length_8920_cov_6.852228"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1489
                     /locus_tag="PLE39_16745"
     CDS             <1..1489
                     /locus_tag="PLE39_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70034.1"
                     /translation="FFARLNEEDSEINLLMGSRSFYEGWDSNRPNVITFINIGTGSEA
                     RKFVLQSVGRGVRIEPMKGKRKRLENLHNANEVDDMLFGQAKPFLAAVQSLFIFGTKR
                     EALETIFEGLAQEKEKEEGVELELSVNPDAISNYPVLIPVYRKLNGHLLVEQHDPRKF
                     EIQASESALLKQYVEYLGDERLLLARHGMTPQQIGLLDETLKSPDSYFNPNTQKKYGN
                     INLLLPRLTQYFGLIPQEVEGFKPLEDEINHFKHIRVLLKDLLEELKKKIEAVRLYKD
                     PADAEKNLKKELKDGTLDIDAYTLAVKNLARTEPSEIFEHNGQRLHIKNIASHYYLPL
                     LVSNDDRLDYISRVIKVESERKFVNQLEAYIKDENHLLNQYDWWMFSRAEETLDKLTI
                     PYYDPTSNKMRDFHPDFVFWLVKGNEYTVLFVDPKGFQNTGYQYKVDGYKDLFREKNG
                     KHKIFKRGKFLVRVALSLYTDDSNQPPAGYREFWYDNPKRIFENL"
     gene            1605..1829
                     /locus_tag="PLE39_16750"
     CDS             1605..1829
                     /locus_tag="PLE39_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70035.1"
                     /translation="MAYTLDTKVGEILDDTGAVKILEKHVPGVSKNPLIGLASGMTLK
                     QILSMPQAKEAGLTEEMVKKVLAEINALKK"
     gene            2250..2750
                     /locus_tag="PLE39_16755"
     CDS             2250..2750
                     /locus_tag="PLE39_16755"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HNE70036.1"
                     /translation="MSKQNINKLMLDLGTFITLLVVSAPRFTGETIHEWLSLALSGAI
                     VMHLILNWNWIVEITTRLFTKSAKNSRFNYVLNWALFASGIMIMLSGLMISKSVVPFF
                     GLTLPQNMSWKELHEVSTNITMILMGLHIAQHWSWITNMFKRIFVPRIPAQTVTPAMS
                     LHRKDA"
     gene            2752..3048
                     /locus_tag="PLE39_16760"
     CDS             2752..3048
                     /locus_tag="PLE39_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70037.1"
                     /translation="MKTLFRILVIMVVAVLIGGAFYSAVTASSSGTDGTSIQERPLPD
                     GFPAERPDGEDFEGGIQFPAEMIKSLVIMTVVATASLNLPKLFNWKKTNLKAGL"
     gene            3230..3748
                     /locus_tag="PLE39_16765"
     CDS             3230..3748
                     /locus_tag="PLE39_16765"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HNE70038.1"
                     /translation="MTTEVVKNTPVQTKTKLWLDVFLFVAFLITMEPHPSGLAIHEWL
                     SLAMVLTMIVHLLLSWDWITEITRRFLGKLGMQNRINYILNWLLFFDGTLLIVSGVMI
                     SEVALPAMGIQLPERFAWRRLHDLSANLGLLMLGIHTALHWNWVVNTVNRYILKPVLG
                     LFSPKQKKDAAI"
     gene            3745..4110
                     /locus_tag="PLE39_16770"
     CDS             3745..4110
                     /locus_tag="PLE39_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70039.1"
                     /translation="MKTLGRTLAILAVFSVTVFLLVTSVNTLGNLIDSDGPRFEQGSP
                     EFRPPEGGENFRPEGGDGDREGGSRGFRINNPRRLATGMVKDTIVVGVLVTAIALPKS
                     FFKKKKRTPKPTATSGGVG"
     gene            complement(4187..5536)
                     /locus_tag="PLE39_16775"
     CDS             complement(4187..5536)
                     /locus_tag="PLE39_16775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE70040.1"
                     /translation="MKLTSKLILAFLVVSLFSTGIIVVITRVATNREFDKFISDQYKA
                     DLVEQLARHYQKYQTWDGLETNFNPFDNDHFRPDDGRPAFFSIADTDGRILVAGAGYR
                     PEEIVSAEKLNKGTPIQVENETVGILLVNTSPEKNPMEDEFIHRLNDSIFLSAVITII
                     LALILGAILSRTITRPIRELTKATHEMADGKLGQQVPVRSRDEIGELASSFNKMNGDL
                     ARSLNLRKQMTADIAHELRTPLSLILGHAEGVHDGVLPPSHENFEIIREEAERLEKLV
                     NDLRTLSLADAGELSVDFQPVDINELLNDVKTHYMTLFNQKRITLNLKPDSATLRVKV
                     NLDPIRFSQVLTNILDNALRYTPENGQVTMATTKVENQIEVSVQDDGEGVPPEDAAHL
                     FDRFYRADPSRTRDAGGSGLGLAIAKSIIEMHQGKIWAESEKGKGMKIIIRLPLSRN"
     gene            complement(5539..6222)
                     /locus_tag="PLE39_16780"
     CDS             complement(5539..6222)
                     /locus_tag="PLE39_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE70041.1"
                     /translation="MPQTILIVDDEKRLVSLVQSYLMQEGYRVVTAYNGKEALPIAQK
                     EKPDLIILDIMMPEMNGYDFMRTHRTEADTPIIMLTAKVEDDDKVIGLELGADDYVTK
                     PFKPRELMARVRNVLRRTGKNEAKAKTLRAGEITVNRDTREVLVVDRSIDLTPSEFDL
                     LTALMSSPGRVFSRLDLLDVIQGVRYEGYERTIDTHVKNLRAKIENDPRKPRYIETVY
                     GVGYRLNKG"
     gene            complement(6388..6855)
                     /locus_tag="PLE39_16785"
     CDS             complement(6388..6855)
                     /locus_tag="PLE39_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70042.1"
                     /translation="MKNRKWLTYTLGTLLTLIVLAAVGGAGFRIGMMQNVSAMTGRPA
                     FAHNFDGDFQPPMQGNHPNDGNSKNGQMNPHQDNFRGNDRGGGPRFFSPIFGLIRLAA
                     LAALLWLGYKFVQKSGWKLTRVTQAQAATETPSAPVETESAAPVNSDENKDEA"
     gene            complement(7016..8503)
                     /locus_tag="PLE39_16790"
     CDS             complement(7016..8503)
                     /locus_tag="PLE39_16790"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HNE70043.1"
                     /translation="MKSQIFHVFTFNAGKCIEGCRDCVKACQVKNQLPEGVTWRRVVE
                     VREADSFLYNLSIACNHCTHPKCAGVCPTDAYFVRNDGIVSIDPLKCMGCGYCAWACP
                     YNAPRYNPTQGQMTKCNFCADSLDAGLPPACVAACPQQALGYEKIDGERAVPNENHWL
                     DLWKTPASAHPFPLPTHSRTRPHLAITPTPAMTSPTETRSYNHEELKPKNIKSELPLV
                     SFTLLIQTAVGGFWSALIWSLLTPNSIDGRLIFTSTLISILLATLGLASAFLHLGKPK
                     NAWRSVQHWKKSWLSREILFTASFVIFGLVTVHQSGIAYREIPMLYSWYGITHWSLVH
                     PCFTGELSCNFILPSAATVQVWMLVTSILGAASVYSMARVYRLHIIAAWDTWRTTAGF
                     FVTTALLGQATILVLAFIGNTQLNWGIITALLVIQLGLWLQTPHQERGHIVRALLIVS
                     ALCGAAALTMTGNHFWLGVPVIFSILIEETLGRKVFYQALTDKAL"
     gene            8637..>8920
                     /locus_tag="PLE39_16795"
     CDS             8637..>8920
                     /locus_tag="PLE39_16795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE70044.1"
                     /translation="MEQVLPSQARVVIIGGGVIGCSTAYHFAKLGWTDVVVLERKQLT
                     SGTTWHAAGLVVCGGFYTETMLELAHYTRELYRNLEKETGVSTGFKPVGYV"
BASE COUNT         2227 a         2142 c         2278 g         2273 t
ORIGIN      
        1 tttctttgcc cgtctaaatg aagaagacag cgagattaac ttgctaatgg gctcgcgcag
       61 cttctacgaa ggctgggact ccaaccgtcc caacgtcatt acctttatca acatcggtac
      121 aggcagtgaa gcccgtaaat ttgtattgca atccgtcggg cgcggcgtcc gcattgaacc
      181 gatgaaaggc aaacgtaaac ggctggaaaa tctgcataac gccaacgaag tggacgacat
      241 gctgtttggg caagcaaaac ccttccttgc cgctgtgcaa tccctgttta tcttcggcac
      301 caagcgcgaa gccctcgaaa ccatctttga agggctggca caagagaagg aaaaagaaga
      361 aggtgttgaa cttgaattaa gcgtcaaccc agatgcaatc tccaattacc ctgtcttaat
      421 tcctgtctat cgcaagttga atggtcattt actggtcgaa caacacgatc cacgcaagtt
      481 tgaaatccaa gccagtgaaa gcgccctgct caagcaatat gtggaatatc ttggtgatga
      541 acgtttattg cttgctcgtc atggcatgac cccgcaacag atcggtctgc tggatgagac
      601 cctgaaatcg cctgatagct atttcaaccc aaatacgcag aaaaaatatg gcaatatcaa
      661 ccttcttctt ccgcgcctaa cccaatactt cggtctcatc ccgcaggaag tggaaggctt
      721 taaaccgttg gaagatgaaa tcaatcactt caaacatatc cgcgtgttac tgaaagacct
      781 actcgaagag ctaaaaaaga aaatcgaggc agtcagacta tacaaagacc ctgctgacgc
      841 cgagaaaaat ctcaaaaaag aactaaaaga tggaaccctt gatattgacg catacacctt
      901 agctgtcaag aatttggcac gtacagagcc atccgaaatc tttgaacata atggtcaacg
      961 cttgcatatc aagaatatcg cctctcatta ttaccttccc ttgcttgtct caaacgatga
     1021 cagactggat tacatctcgc gcgttatcaa agtggaaagc gagaggaaat ttgtcaacca
     1081 attagaagcc tatatcaagg acgaaaacca cctcttgaat caatacgatt ggtggatgtt
     1141 cagccgtgcc gaggaaacgc tcgataaact taccatccct tactacgacc ccacgtcaaa
     1201 taaaatgcgc gacttccatc ccgactttgt cttttggctc gtgaaaggca atgagtatac
     1261 agtcctattt gttgatccca agggtttcca aaacacaggc tatcaataca aagtggatgg
     1321 ctacaaagat ttattccgag aaaagaacgg gaaacacaaa atattcaagc gcggaaagtt
     1381 cttagtgcgc gtggcattga gtctatacac agatgacagc aaccagccac ctgctgggta
     1441 tcgtgagttt tggtacgata atccaaagag aatatttgag aatctatgat caggggtatc
     1501 agtgcgcaga tgtgggagaa agggcgctga atccttgtcc ctgccgtata atagatgtgc
     1561 aggttttgac cattccatat tttcattgca caaggagaga aaaaatggca tatacattgg
     1621 acacaaaagt tggcgaaatt ttggatgata ctggcgctgt gaagattctt gaaaagcatg
     1681 tgcctggtgt ttccaaaaac cctttgatcg ggttggcttc aggcatgact ttgaagcaaa
     1741 tcctatccat gcctcaggcg aaagaagcgg ggttgactga ggaaatggtt aagaaggttt
     1801 tggcggagat caacgccctc aagaagtaaa ccgcttttac gaaaaccaat acgcaaaagg
     1861 cttcagaagc tgaaacgctt ttgaggcctt tttgttgatt tggtctcttg cacaagctgc
     1921 aagaaatgtc tttgaaaagc cattttgaac cacaaagaca ctttattaat tgttttctct
     1981 gtgtctccgt gtctccgtgg ttgaactttt agacctttgc aagtgacata ttggggcagt
     2041 gccaaactgt ggggttgtga actgcaaagc ccgcgaagag cgtcaggaaa ccataacatc
     2101 tttgcgaatt ttgcgttctt cgcgatggta ctgcctttgt gttgtttgtg tactgtttta
     2161 caagttcttc atatttctgc cgtatttcca ccacccagtt gaagctccct gcttatagaa
     2221 tggcggcatc aataaacaag gagacacaca tgtcaaaaca aaacataaac aaactcatgc
     2281 tcgatctggg cacatttatc accctgttag tggtcagcgc gccgcgcttt acaggcgaaa
     2341 ccattcacga atggctgtca ttggccctga gcggggccat cgtaatgcac ctgattttga
     2401 actggaattg gatcgtggag atcaccaccc gtttgttcac gaaatcagca aagaactcgc
     2461 gcttcaacta tgtcttgaac tgggccctgt ttgccagcgg aattatgatc atgctctcgg
     2521 ggctgatgat ctccaagtcg gtggtgccct tcttcgggtt gaccctgccg cagaacatgt
     2581 cgtggaagga attgcacgaa gtctctacca acatcaccat gatcttgatg ggcctgcaca
     2641 ttgcccaaca ctggagctgg atcaccaaca tgttcaagcg cattttcgta ccgcgcatcc
     2701 ctgcgcagac tgtaactccc gccatgtcat tgcacagaaa ggatgcctaa aatgaagact
     2761 ctgttcagaa ttctcgtgat catggttgtg gctgtgttga tcggcggcgc tttctacagt
     2821 gcagtgaccg cttcctcctc agggacagat ggcacatcca ttcaggaacg tccactcccc
     2881 gatggtttcc ccgcagaacg gccagatggt gaagatttcg aaggcggaat ccagtttcca
     2941 gccgaaatga tcaaaagcct ggtgatcatg accgtcgttg caaccgcctc actgaacctt
     3001 ccaaaacttt tcaactggaa aaaaaccaac ttgaaagcag gcctgtaacc ctttttcgca
     3061 cattccccga ccaaccagtt ggggaatgtg tttacaactt cttcacaatt aatccgcaaa
     3121 acccccatca caaaatataa taagctcata aaattgggca gttcacaaga ccgcctacaa
     3181 acccataaga cactgctcgc agggttggca atgaatttca aggagaaaca tgaccacaga
     3241 agttgtaaaa aatacaccag tccaaaccaa aaccaagttg tggctggatg tctttctttt
     3301 tgtcgcgttt ttgatcacga tggagccgca cccaagcggg cttgccattc atgaatggtt
     3361 gagcctcgcc atggtgttga cgatgatcgt ccacctgctg ttgagttggg attggatcac
     3421 ggagatcaca cgccgttttt tgggaaaact cgggatgcag aaccgcatca actacatctt
     3481 gaactggctg ctgttcttcg acgggactct gctcatcgta tctggtgtga tgatctcgga
     3541 agtggccctg cctgcgatgg gcattcagct ccccgaaaga tttgcctggc gcagactgca
     3601 tgacttgtcg gccaaccttg gcctgctcat gctgggaatt cacaccgccc tgcattggaa
     3661 ttgggttgtc aatacggtaa accgctatat actcaagcct gttctgggct tgttttctcc
     3721 gaagcaaaag aaggatgccg caatatgaaa acgctcggac gcacccttgc cattctggct
     3781 gtgttttctg taacggtgtt cctgctcgtg acctccgtca atacacttgg gaacttgatc
     3841 gactcagacg gtccacgttt tgaacaaggc agccccgaat tcaggcctcc tgaaggcggc
     3901 gaaaactttc gacccgaagg cggcgatgga gaccgtgaag gcggttcgcg tggattcagg
     3961 atcaacaatc cgcgccgact ggcaacaggc atggtgaaag atacgatcgt ggtgggcgtt
     4021 ttagtcaccg ccattgccct gccaaaaagt tttttcaaaa agaaaaagag aacccccaag
     4081 ccaactgcca caagcggtgg agtgggctaa gcatacgcaa agacgtccgt gaaatgaaaa
     4141 tttcacggac gtcttttttt gtatttccac actgtaagtt gggcgctcaa ttccttgata
     4201 atggcaggcg gatgatgatc ttcatccctt tgcctttttc gctctctgcc cagattttgc
     4261 cctgatgcat ttcgatgatg gatttggcaa ttgcaagtcc cagccccgag ccgcctgcat
     4321 cgcgggtgcg cgaggggtcg gcgcggtaga aacggtcaaa gaggtgggcg gcatcttcag
     4381 ggggcacacc ctctccatcg tcttgaacgg aaacttcgat ctgattctca accttggttg
     4441 ttgccattgt gacttgtccg ttttctgggg tgtaacgcag ggcattatcc aggatgttgg
     4501 tcagtacttg agagaagcgg atcgggtcaa gattcacttt cacgcgaagc gttgccgaat
     4561 caggcttcag gttaagagtg atccgctttt ggttgaacag ggtcatgtaa tgggttttga
     4621 catcgttcaa tagttcgttg atgtccacgg gttggaaatc caccgaaagc tcgcctgcat
     4681 ctgcaaggga aagtgtacgc aggtcgttga caagtttttc cagccgttcg gcttcttcgc
     4741 ggatgatctc aaagttttca tgcgagggcg gcagcacacc atcatggacg ccttccgcat
     4801 ggccgagaat caaactgagg ggagtgcgta gttcgtgcgc aatgtcggcg gtcatttgtt
     4861 tgcgcaggtt gagcgaacgc gcgaggtcgc cattcatttt gttgaaagag gatgcgagtt
     4921 cgccgatctc atcgcgtgag cggacaggca cttgttgccc cagtttgcca tcggccattt
     4981 catgagtggc tttggtcaat tcacggatgg ggcgtgtgat ggtgcgcgaa aggatcgccc
     5041 caagaatcaa ggcgaggatg atggtaatga ccgcgctgag aaaaatggaa tcgttgaggc
     5101 gatgaatgaa ttcatcttcc atcggattct tttctggcga agtgttaacc aacaggatgc
     5161 ccaccgtttc attctcaact tgaattgggg tgcccttgtt gagtttttcg gcgctgacaa
     5221 tttcttcggg gcggtatccc gcgcctgcaa ctagaatcct gccgtctgta tctgcgatgg
     5281 agaagaaggc ggggcggccg tcgtcaggtc tgaagtggtc gttgtcgaac gggttgaaat
     5341 ttgtctccag cccatcccag gtttggtatt tttgataatg acgggcaagt tgttctacca
     5401 gatctgcttt gtactggtcg ctgatgaatt tgtcgaactc gcggtttgtg gcaacgcgtg
     5461 tgatcacaac aataatgcct gtactgaaca ggcttacgac caaaaacgcg aggatcaatt
     5521 tggaggtcag tttcatgcct agcctttatt caggcgatag ccgacgccat acacggtttc
     5581 gatatagcgc ggtttgcgcg gatcattttc gatcttggcg cgcaggtttt tgacgtgcgt
     5641 atcaatggtg cgctcgtagc cttcataacg aacaccctgg atcacatcca acaggtcgag
     5701 gcgagagaac acacgccccg gagatgacat gagcgccgtg agcaggtcaa actcggatgg
     5761 cgtcaggtca atgctgcggt ccaccaccag cacctcgcgg gtatctcggt tgactgtgat
     5821 ctcgcctgcc cgcagggtct tggccttggc ttcgtttttc cctgtgcggc gcaacacatt
     5881 gcgcacacgc gccatgagtt cacgcggctt gaagggcttg gtcacataat cgtccgcgcc
     5941 cagttccagg ccgatcacct tgtcatcgtc ttccactttg gcggtcaaca tgatgatggg
     6001 agtgtcggct tcggtgcggt gggtgcgcat aaaatcgtac ccgttcattt cgggcatcat
     6061 gatgtccaaa atgatgagat cgggtttttc tttttgagca atgggcaatg cctctttgcc
     6121 gttatacgcc gtgacgacac gatacccctc ttgcatcagg taactttgta caagggaaac
     6181 aaggcgtttt tcatcgtcaa cgatcaggat ggtttgtggc atgggcattc tctctgttta
     6241 atggcctgta tttgaaggtt tgttggtcgt attatctcat aaggcatggg gatgtagctg
     6301 tgaagaaatt atgtgctttt gtggagaaaa cacccgcctc gatagataga ccgaagcggg
     6361 tgtttgattc tgttcaaaag gctgactcta agcctcgtcc ttattctcgt cactgttgac
     6421 aggggctgcg ctttcggttt caacgggtgc cgaaggcgtt tctgtggctg cctgtgcttg
     6481 tgttacacgg gtcagcttcc agccgctctt ctgcacaaac ttgtacccga gccagagcag
     6541 ggccgccagc gccgccaaac ggatcagtcc gaagatgggc gaaaaaaagc gcggaccgcc
     6601 gccacggtca ttgccgcgga agttatcctg gtgcggattc atctgtccgt ttttggaatt
     6661 gccgtcattt gggtgatttc cctgcatcgg cggctggaaa tcgccgtcaa agttgtgagc
     6721 aaatgcgggg cgtcccgtca tggctgagac gttttgcatc atcccgatgc ggaagcccgc
     6781 gccgcccacg gcagcaagga cgatcaaggt taacaaggtt cccagagtat aggtcaacca
     6841 ttttctgttt ttcattcttt ttctccagta ataatttttg tgtggccctg cctgtatgag
     6901 caggggataa agcgaatgct ttattgaatc ttattttcct actggagaat tacataaggg
     6961 tggaggaatt gtgtggaact tgtgtgttca aaattgaagt gctggcgaaa acaggctaca
     7021 acgccttgtc tgtcagcgct tggtaaaaaa cctttcgtcc aagagtctct tcgatgagaa
     7081 ttgagaaaat gacaggcaca ccaagccaaa agtgattgcc tgtcattgtt aatgccgccg
     7141 ccccgcacaa ggctgaaacg atcagcaatg ctcgaacgat gtgccctctt tcttggtgcg
     7201 gagtttgcag ccacaacccc aattggatga ccaacaaggc agtgatgatg ccccaattca
     7261 attgggtatt cccgataaac gcaagaacca atatggtggc ctgcccaagc aaggcggtgg
     7321 tgacaaaaaa gcccgccgtg gttcgccatg tatcccatgc ggcgatgatg tgcagacgat
     7381 aaacccgagc catgctgtaa acgcttgcgg ccccaagtat ggatgtgaca agcatccata
     7441 cttgcacagt cgctgcactg ggaaggatga aattgcagga aagctcacca gtgaaacaag
     7501 gatgtacaag gctccaatgg gtgattccgt accatgaata cagcatgggg atttccctgt
     7561 acgcgattcc gctttggtgg acagtgacaa gcccgaatat gacaaaagat gcagtaaaca
     7621 gtatttcgcg gctgagccat gattttttcc aatgctgcac acttctccac gcattttttg
     7681 gtttgccgag gtggaggaag gcacttgcca gcccaagcgt tgccaaaagg atggagatca
     7741 gagttgaggt gaatatcaat cttccgtcaa tgctgtttgg ggtgagaagg ctccagatca
     7801 atgcgctcca aaatccaccg accgccgttt gaattaaaag agtaaatgac acaagcggca
     7861 gttcgctctt tatgtttttt ggtttgagct cttcgtggtt gtaacttcgc gtttccgttg
     7921 gcgaggtcat tgctggggtg ggagtgatgg caagatgcgg gcgagtccgt gaatgagttg
     7981 gcagtggaaa aggatgggcg cttgctggcg ttttccataa atccagccag tgattttcgt
     8041 ttgggacagc tctttcgccg tcaatttttt cgtagcctaa tgcctgctgc gggcaagccg
     8101 ccacacatgc aggcggaagc cctgcatcca gactgtcggc gcagaaattg cattttgtca
     8161 tctgaccctg tgtcgggttg tagcgaggcg cattgtaagg gcaggcccag gcgcaataac
     8221 cacatcccat gcatttgagc ggatcaatgc ttacaattcc atcgtttcgg acaaaatatg
     8281 cgtcggttgg gcatacccct gcgcattttg gatgcgtaca atgattgcat gcaatggaca
     8341 ggttgtataa aaaagaatcc gcctcacgca cttccaccac gcgccgccat gtgacacctt
     8401 cagggagttg atttttcacc tgacaggctt tcacgcaatc tcggcaacct tcaatgcatt
     8461 tgcctgcgtt gaaggtgaag acatggaata tctgtgactt catgtggtaa gtgtaaagtg
     8521 aaggcgtgtg gacaggaagt gatggatgta acttgttttt cctcttgccc caaatataaa
     8581 attcctgtat ccttggctgg acggtgaaat ccatttcagc aacacaggag cggtaaatgg
     8641 aacaggttct tccttcacaa gcacgggtgg tcattattgg cggcggggtg atcgggtgca
     8701 gtacagcgta tcattttgca aaacttggct ggacggatgt cgtcgtcctt gaacgaaaac
     8761 aactcacctc tgggacaaca tggcatgcgg cagggttggt ggtgtgcggc gggttttaca
     8821 ccgagaccat gctcgaactt gcgcattaca cccgtgaact gtatcgcaac cttgaaaaag
     8881 agactggcgt ttccacaggc ttcaaacctg ttgggtatgt
//
LOCUS       DAOQJC010000150         8625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_4391_length_8625_cov_7.195333, whole genome shotgun sequence.
ACCESSION   DAOQJC010000150 DAOQJC010000000
VERSION     DAOQJC010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8625
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4391_length_8625_cov_7.195333"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLE39_16800"
     CDS             <1..222
                     /locus_tag="PLE39_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70045.1"
                     /translation="TMIQKLPAEQAAEVAEDFGKLADEAVKPKPNKRWYSVSIEGLIK
                     AAENLDKLGTPVISLSRKVLSLLMGGMVK"
     gene            265..477
                     /locus_tag="PLE39_16805"
     CDS             265..477
                     /locus_tag="PLE39_16805"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNE70046.1"
                     /translation="MEAVTISSKYQVVIPREVRQQFNLKPGQQIVFIPYNGTLRVVIV
                     PPVKKMRGMLKGMDTKNIREEVDEER"
     gene            477..857
                     /locus_tag="PLE39_16810"
     CDS             477..857
                     /locus_tag="PLE39_16810"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE70047.1"
                     /translation="MNVVDSSGWIEYAADEENADFFERPISDRDNLLVPTVCIYEVFK
                     RVLREFGEERALEVIGQMSLGEVVELDRQIAINAAQISTELKLAMADSIILATARLRN
                     ATLWTQDEHFKDLDGVKYIEKKYS"
     gene            995..2017
                     /locus_tag="PLE39_16815"
     CDS             995..2017
                     /locus_tag="PLE39_16815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE70048.1"
                     /translation="MSLIHVNNLTKHFKILNRREGLSGAFRDLFSGDYRTVKAVDGIS
                     FDIEAGEIVGYIGPNGAGKSTTIKMMTGILKPSSGEIRINNHIPFENRIRQAQIMGVV
                     FGQRTQLWWDLPVIESFRILKEIYKVPQKTFDEHMGMFNELVGLKALYSQQVRTLSLG
                     QRMLCDITASFLHNPQIVFLDEPTIGLDISIKAKIRTVIKELNEARKTTIILTTHDLG
                     DVEALSQRVIIIDKGKKLYDGDTKKVNALFGAYRTLKVQVNDFNEGTLDALKNKLAAQ
                     FGATNKIVIAQTEEFWTDITIDQAATPLSEVLNCVMTNFSILDVRIVEIAMEDVVQRI
                     YDGALS"
     gene            2014..2805
                     /locus_tag="PLE39_16820"
     CDS             2014..2805
                     /locus_tag="PLE39_16820"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNE70049.1"
                     /translation="MRKYLAFTRGSFMVGLVYRFGPLLALIGNFVYLGVAYYLWHSIY
                     KNSDTLRSMTFNETFLYVAIGSAVFILLKTYVDWEVSYEIREGIISTYLTKPLDYQTY
                     ILFTSLGQTLMSLMVITLPTVLVVTLVFKVSIPLGIGTLLFPFSLLLAFVISFCFDYF
                     TGILAFYTESVWGLSTTKEIIILALSGALIPLQFFPAKLQSILLLLPFQTIYHTPLMM
                     LTRPNQDVRTFVGMLIVQGVWAVVLYVLTRLFFEQAVKVLRVAGG"
     gene            2808..3605
                     /locus_tag="PLE39_16825"
     CDS             2808..3605
                     /locus_tag="PLE39_16825"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNE70050.1"
                     /translation="MRSIGEYLRLYFIIEAQYIKARMQYRADFIISSIGMFFSSLVTL
                     GVFWVIFDTVPDLAGWSLMEMAFIYAFYMIAISPMQVIFDHFWQLRFNLQQGNFLKYY
                     FRPLNMMFYYTSEMFDLKGLTQFFVGIALLVYTANQLHVTWTFARVVLLFVTLFSAAL
                     VQISIVVLAACSAFWILDSYPVLGLAWRLREFAPYPMTIFDGAFRFTFTYILPIGFIA
                     FYPSQIFLRPEDVSTLTYFSPIVGIGLFAFTYWVWKKGVNSYTGTGS"
     gene            3602..4453
                     /locus_tag="PLE39_16830"
     CDS             3602..4453
                     /locus_tag="PLE39_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70051.1"
                     /translation="MKKAFYSLLITLLLAGCNTSQAHGPAPLEAGYRYSSYGPTSNPG
                     AEYWPEVGQRIASEFENATPATIWIVGVVDGEGSYLNFPCETDDPNIHCGPVDMNESM
                     LTTFDEQGFDVWLQVEAGNADMDELIDIVLGQYEHHPSVVGFGVDVEWYKSSQVPQGE
                     PVSDEVAQAWVRAIRNHHPEYQLFLKHWDTAWMPPTARDGILFINDSQGFESFEQMTT
                     EFAEWGKTFAPARVGFQYGYQADQRWWSEMQNPASEIGHVLLEKIPNTQALFWVDFTI
                     LETFPPE"
     gene            4499..5425
                     /locus_tag="PLE39_16835"
     CDS             4499..5425
                     /locus_tag="PLE39_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70052.1"
                     /translation="MRSSDIKYLSFGLGIGALVASLFFIGFLFIPQSNPPADTLIAGI
                     KNTLVPATATPQNTETSTPAPTAEGTLTPTFTNTPTAQPTSTPLPTATATLSTAQIMI
                     TTGEIVPAGPLSLEQQIKLYEASLFFIAPTYEQSKQMSVLINHVRFSDPSNTCGPLSI
                     AILQKAGIISETTLEAHDFFLINPDLGKDQITIKTAFPSERYTNTRYKVKINQFDWRA
                     NPLLPGDFVYIYSGTEGNFEHMLVVNRVDSQGRVYSVTNYNTEQGFVITEVMLYDPSD
                     PTLGMFAQWTAKPRAKLGSTGFAGFEVWRLKQ"
     gene            5426..6028
                     /locus_tag="PLE39_16840"
     CDS             5426..6028
                     /locus_tag="PLE39_16840"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNE70053.1"
                     /translation="MDSAITTFIFDFGNVFVKWDIHALYNRFFPNPQAVDSFLEEIRF
                     HEWNAHQDAGRPFKDGIAILSEKYPHYADLIQAYDTHWEDSITDMYHGTVEILRRLKG
                     EGWPIYLLSNFSAEKFPLMRERYEFLNLFDDMIISGEHKLIKPDPAIFQLTLERIDRT
                     ANECLFIDDSLANIETARNMGFHTIHFQSPEQLERDLKIW"
     gene            6072..7118
                     /locus_tag="PLE39_16845"
     CDS             6072..7118
                     /locus_tag="PLE39_16845"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNE70054.1"
                     /translation="MTNIQKTMLKAVEEELQRQVARLDQPRTLPFHEMLTYHMGWTGD
                     GAGPEATGKRIRPLLVLLSVASCEETTTTTSTSATSAPSWLRAKSAAAAVELVHNFSL
                     VHDDIQDNSDKRRGRDTVWVKWGAPMAINAGDALFVIANQAIVDLIEFYPAEVVAKAA
                     KILNDTCLDLTRGQFLDMSYEEHSDLGIDDYWPMVSGKTSALLSACTHIGALLGGADE
                     VKQEAYRLFGHHLGLAFQVQDDILGIWGDEAVTGKSAASDLVEGKNSLPILFGLGKQG
                     DFAKRWAQGPISPSEVKAVAAQLEEEGGKSYATEVAEKETQKALEFLNKADPKGEAGQ
                     SMLDLANLLLKRKQ"
     gene            7200..7271
                     /locus_tag="PLE39_16850"
     tRNA            7200..7271
                     /locus_tag="PLE39_16850"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7232..7234,aa:Gly,seq:ccc)
     gene            7338..8258
                     /locus_tag="PLE39_16855"
     CDS             7338..8258
                     /locus_tag="PLE39_16855"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNE70055.1"
                     /translation="MSPRIATTQKWYLASLALTDAYTDFILSRQAMQCAPTTLEFYKH
                     TAGVFLNWTEGQGITSPEQVDARLVRHYLADLTTKGKSDKTLHAHARAIRTLLRFWHA
                     EKYIPEAVTFSMPKIEKKRLPVLSADELNKVLTACKNPRDKALILFMADSGLRRAEIT
                     ALNWGDADIMSGLVRVKRGKGGKARSAVIGATTRRALLAYRRTLGSPSNDAPMFISRS
                     GGRFTGTGVLLIFRRLTEKTGIHITPHALRRTFVILSLRAGMDVLHLQAMLGHASLEM
                     VQHYAQMVDDDLLQAHQAHSPMDNLRQLTQ"
     gene            complement(8311..>8625)
                     /locus_tag="PLE39_16860"
     CDS             complement(8311..>8625)
                     /locus_tag="PLE39_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70056.1"
                     /translation="YTITRASKEKVDCKYPIDLTTTALPWGGLRYWFICPNLACGRRV
                     SVLYLAPGGTYFVCRHCNRLSYRSRQEGYQDRAFYKRMAGMMQDLFPGITGERVKEIF
                     GE"
BASE COUNT         2218 a         2290 c         2052 g         2065 t
ORIGIN      
        1 accatgattc aaaaactccc cgccgaacaa gccgccgaag tcgccgaaga ttttggcaaa
       61 ctggcggatg aagccgtcaa gccaaaaccc aataagagat ggtactcggt cagtattgaa
      121 ggtctcatca aggcggcgga gaatctggat aagttgggca cgcctgtgat cagtttgtca
      181 cggaaggtat tgtccctgct gatgggcggg atggtgaagt gatataatcg ctttacgatt
      241 tcttttcgat aaggagaaag taaaatggaa gcggttacaa tttcaagtaa atatcaagtt
      301 gtcatacccc gtgaagtgcg gcaacaattc aatctcaaac ctgggcagca aatcgtcttt
      361 atcccctaca atggaacact gcgagtggtc attgtccctc ctgtaaaaaa aatgcgtggg
      421 atgttgaagg gaatggatac aaagaacatc cgcgaagaag tggatgagga gcgataatga
      481 acgtcgttga ttcatcaggc tggatcgagt atgcagccga cgaagaaaat gcggattttt
      541 tcgaacgtcc catcagtgac agggataatt tgcttgttcc cactgtctgc atttacgaag
      601 tcttcaaacg agtcttgcgc gaatttggcg aggaacgcgc actggaagtc atcggtcaaa
      661 tgtcattggg agaagtggtg gaactggatc gccaaatcgc catcaacgcc gcgcaaattt
      721 ctacagaact aaaacttgca atggcagata gcatcatcct cgccaccgcc cgcctacgca
      781 acgccaccct ttggacacag gacgaacact tcaaagacct cgatggggtg aaatatatcg
      841 aaaagaagta ttcttgaaac gtctcatcaa agccgccgag aatttggata agttgggtac
      901 gcccgtgatc agtttgtcac agaaggtctt gtccctgctg acgggcggga caacctcatg
      961 agcctccgcc agattacggt gtaaaatcca gttcatgagc ctcattcatg tcaacaacct
     1021 gaccaaacat ttcaaaatac ttaaccgccg cgaaggttta agcggcgcat ttcgcgacct
     1081 gttttcgggg gactatcgaa ctgtcaaagc agtggacggc atctcatttg atatagaagc
     1141 aggtgaaatc gttgggtaca ttggaccaaa cggcgcgggc aaatccacca ccatcaaaat
     1201 gatgacgggg attctcaaac cttcaagcgg cgagattcgc atcaataacc acatcccgtt
     1261 tgaaaatcgg atccgtcagg cgcagatcat gggagtggtc tttggtcaac gcacgcaatt
     1321 gtggtgggac ctgcccgtca ttgaatcgtt caggatattg aaagaaatct acaaggttcc
     1381 gcaaaaaacc tttgacgagc atatgggcat gttcaatgaa ctggtcgggt tgaaggcctt
     1441 gtattcgcag caggtgcgca cactctcgct cggtcagcgc atgttgtgcg atatcaccgc
     1501 ctctttcctg cacaacccac agatcgtatt tctggatgag ccgaccatcg ggctggatat
     1561 ttccatcaag gccaagatcc gtaccgtcat caaagaactg aacgaagcgc gcaagaccac
     1621 catcatcctc accacgcatg accttggcga tgtcgaagcc ttatcgcagc gtgtcatcat
     1681 tattgacaag ggcaaaaagc tgtacgatgg cgacacaaag aaggtcaatg ccctgtttgg
     1741 agcgtatcgc acgctgaaag tacaggtcaa cgatttcaac gaaggcacgc tcgatgccct
     1801 caaaaacaaa ctcgccgcac agttcggcgc aaccaacaag atcgtcattg cccaaactga
     1861 agaattctgg acagacatca ccattgacca ggccgccact ccgctctcgg aagtattgaa
     1921 ttgcgtgatg accaacttct ccattctgga tgtccgcatc gtggaaatcg ccatggagga
     1981 tgtcgttcaa cgaatttatg atggagccct gtcatgagaa agtatctggc attcacacgc
     2041 ggctccttca tggttgggct tgtttatcgc tttggtccgc tccttgcgct gatcggaaac
     2101 tttgtttacc tcggcgtcgc gtactatctc tggcacagca tttacaaaaa ctccgacaca
     2161 ctgcgcagca tgaccttcaa cgaaactttt ttatatgttg ccattggctc cgcagttttt
     2221 atcctgctca aaacctacgt ggattgggaa gtctcatacg agatccgcga aggcatcatc
     2281 tccacctacc tcaccaaacc gctggattat cagacctaca ttctttttac cagcctcggt
     2341 caaacgctga tgagcctgat ggtgatcacc ctgccgactg tgctcgttgt gacattggtt
     2401 ttcaaggtga gtattccact ggggattgga acgcttctct ttccctttag cctgcttctg
     2461 gcattcgtca tcagtttctg cttcgactat ttcacaggca tcctcgcgtt ctacaccgag
     2521 tcggtttggg ggctgagcac caccaaggag atcatcatcc ttgcgctttc tggcgccttg
     2581 attccactgc aattcttccc tgcgaaactg caaagcattt tgttgctgct gccctttcaa
     2641 acgatttacc ataccccatt aatgatgctg accagaccca accaggatgt gcggacattt
     2701 gtcggcatgt tgatcgttca aggtgtatgg gcagttgtcc tatacgtttt gacccgtctc
     2761 ttcttcgaac aggcggtcaa agtcttgcga gtcgcgggag gctaaccatg cgcagcattg
     2821 gcgaatatct gcggctgtac ttcatcattg aggcgcaata catcaaggcg cgcatgcaat
     2881 atcgtgcaga tttcattatc agctcgatcg gaatgttctt ttcaagcctg gtcacactgg
     2941 gggtcttttg ggtcatcttc gacaccgtac ccgaccttgc aggctggtct ttgatggaaa
     3001 tggctttcat ctacgcgttt tacatgatcg ccatctcgcc catgcaagtc atcttcgacc
     3061 acttctggca gctgcgcttc aatttgcagc agggaaattt tctgaagtat tacttccgtc
     3121 cgttgaacat gatgttctac tacacctccg aaatgttcga cctgaagggg ctgacccaat
     3181 tctttgtggg catcgcttta ctggtctaca cagccaatca gcttcatgtc acatggacct
     3241 ttgcgcgggt ggttctgctg tttgtcacct tgttcagcgc cgcactggta caaatttcca
     3301 tcgtggtgct ggctgcctgc tccgcttttt ggatactaga ctcctacccc gtgctgggac
     3361 tggcctggcg tttgcgtgag tttgccccct accccatgac gatcttcgac ggtgcatttc
     3421 gtttcacctt cacctacatc ctgccaatcg gattcatcgc cttttatcca tcacagatct
     3481 ttttgaggcc agaggatgtt tccacgctga cctacttctc gcccattgtt ggcatcgggc
     3541 tatttgcatt cacctattgg gtatggaaaa agggcgtcaa cagctacaca gggactgggt
     3601 catgaagaag gcattctact cactcctcat cacgttgctg ctggcaggct gcaacacctc
     3661 gcaagcacat ggccccgccc cattggaagc gggataccgc tactcatcct atgggccaac
     3721 atccaaccct ggggcggagt attggcccga ggttggtcaa cgcatcgcat cggaattcga
     3781 gaacgcaacc cccgccacga tctggatcgt aggggtcgtg gatggcgaag gcagttatct
     3841 aaatttccct tgcgaaacag atgacccaaa cattcactgc ggcccagtgg atatgaacga
     3901 atccatgctt accaccttcg acgagcaggg atttgatgta tggctgcaag tggaagcggg
     3961 caacgccgac atggacgaac tgatcgatat tgtccttggt caatacgaac atcatccaag
     4021 cgtcgtcggt ttcggggtgg atgtggaatg gtacaaatcg tcgcaagtgc cacagggcga
     4081 accagtatca gacgaagttg ctcaggcatg ggtaagggcg atccgaaatc atcatccaga
     4141 atatcaactc tttctcaagc attgggatac cgcctggatg ccgcccacag cccgcgatgg
     4201 gattctgttc atcaacgaca gtcagggctt cgaatctttt gagcaaatga caaccgagtt
     4261 tgcagaatgg ggaaagacct tcgcccctgc ccgcgtcggt tttcagtatg gctaccaggc
     4321 cgatcaacgg tggtggagtg aaatgcaaaa ccctgcttcc gagatcgggc atgtgcttct
     4381 tgaaaaaata ccgaacacac aggcgctttt ctgggtggat ttcaccatcc ttgaaacctt
     4441 tccgccagaa tgagcattga ttccaaggtt cgattaaaat aataatattg aaactgacat
     4501 gcgatcatct gacatcaaat atctaagttt tgggctggga attggcgcgc tggtagcgag
     4561 cctttttttt attgggtttc tttttattcc gcaatcgaac ccacctgccg ataccctcat
     4621 agcgggtatt aagaacactc tggtccctgc cacagcaacg ccgcaaaaca cggaaacatc
     4681 cactcctgcc cccacagcag aaggcacact tacgcccaca tttacaaata ccccaactgc
     4741 tcaacccact tcaacgccgc tcccaacggc aaccgccaca ctttccaccg cgcagatcat
     4801 gatcacaacg ggagagatcg tccccgctgg cccgctcagc ctcgaacagc agatcaagtt
     4861 atatgaagca tccttgttct ttattgcacc gacctacgaa caaagcaaac aaatgtcggt
     4921 gctgatcaac catgtccgct tttcagaccc aagcaacacc tgcggcccac tttcaattgc
     4981 cattttgcaa aaagcaggca tcatcagcga aaccacgctg gaagcacacg acttcttcct
     5041 gatcaatcct gatctcggca aagaccagat cacaatcaaa accgcgtttc cgagtgagcg
     5101 ttacacaaac accagataca aagtaaagat caatcaattc gattggcgtg ccaacccgct
     5161 cctgcctggt gatttcgttt acatctactc aggcaccgag ggcaattttg aacacatgct
     5221 cgtcgtcaat cgtgtcgatt cgcaggggcg cgtttattcg gttaccaatt acaacacgga
     5281 gcagggtttc gtcatcactg aggtcatgct gtatgaccca tcggacccaa cgctcggcat
     5341 gtttgcacaa tggacggcaa aaccgcgcgc aaaactcggc tctactgggt ttgcaggttt
     5401 tgaggtctgg agattaaaac aataaatgga ttccgccatc acaaccttca ttttcgactt
     5461 tggcaacgtc ttcgttaagt gggatatcca cgccctttac aatcgcttct ttcccaaccc
     5521 gcaagctgtt gactcttttc tcgaggaaat tcgcttccac gaatggaacg cccatcagga
     5581 cgcaggccgt ccattcaagg atgggattgc cattctttcg gaaaagtatc cgcattacgc
     5641 cgatctgatt caagcctatg acacacactg ggaagacagc atcaccgaca tgtatcacgg
     5701 cacagtggaa attctccgca ggttgaaagg ggaaggctgg ccaatttacc tgctcagtaa
     5761 tttttccgcc gaaaaattcc cgctgatgcg ggaacgttac gagtttctca acctgttcga
     5821 tgacatgatc atctcaggcg agcataaact catcaaacca gatcctgcca tcttccaact
     5881 caccctggag aggatcgatc gcacagccaa tgaatgtctg ttcattgacg attctctggc
     5941 aaacatcgaa accgcacgaa acatgggctt tcacaccatt cacttccaat cgccagaaca
     6001 actggaaagg gatttgaaaa tctggtaaag tcgtgcgagc caaaaccaac tctgcaaaac
     6061 ggaacaccac catgacaaat attcaaaaaa ccatgctcaa ggctgttgaa gaagaattac
     6121 aacggcaggt tgcacgcctc gatcagccgc gtaccctgcc cttccatgag atgctcacct
     6181 atcacatggg ctggacggga gatggcgccg gcccggaagc tacagggaaa agaattcgtc
     6241 ccttgcttgt attactctct gttgcttcct gtgaagaaac caccactact acatccacat
     6301 ctgctacatc cgccccttct tggctacgcg caaagtctgc cgcagctgcg gtggaactcg
     6361 tccacaactt ttcattagtt catgatgata ttcaagacaa ttcagataaa aggcgcggca
     6421 gagatacggt ttgggtcaag tggggtgcgc cgatggccat caatgcaggt gacgcactgt
     6481 ttgtcattgc gaatcaagca attgttgatt tgatcgaatt ttatccagcc gaagttgtag
     6541 caaaagcggc caaaatactg aacgatacct gcctcgacct gactcgcggt caattcctgg
     6601 atatgtctta cgaagaacat agcgatctgg ggattgacga ttattggccc atggtcagcg
     6661 ggaagacctc tgccctgctt tcggcctgca ctcacatcgg cgccttgctt ggcggcgcag
     6721 acgaagtgaa gcaggaagcc tatcgcctgt ttggtcacca ccttgggctg gcttttcaag
     6781 tgcaggacga tatcctcggc atttggggtg acgaggcagt tacaggaaaa tccgctgcca
     6841 gtgatttggt ggaaggcaaa aattccctgc cgattttgtt tgggctgggt aaacaaggag
     6901 actttgccaa acgctgggcg cagggaccaa tcagccccag cgaagtgaaa gcagtagccg
     6961 cccaactgga agaagaaggc ggaaaaagtt acgcaaccga agtggctgaa aaagaaactc
     7021 aaaaagcgct tgaattcctc aataaggccg acccgaaggg cgaggcaggg caatccatgc
     7081 ttgatctggc gaacctgctg cttaaacgga agcagtaaac aggtcaagag cgaggcgcag
     7141 aatttcatct gcttgacatg ccttttccat gtcgcttata atccaatccg ttaaaccaag
     7201 cgggtgtagt tcagtggtag aacgcttgct tcccaagcaa gatatcgtgg gttcgagtcc
     7261 catcacccgc tctaacaagg aaataagagc aaatcaccgt catattttgc caataatggc
     7321 agaaagacgg tgtttttatg tctccccgaa ttgctacaac tcaaaaatgg tatcttgcct
     7381 cccttgccct cacagacgca tataccgact tcattctcag ccgccaagcc atgcaatgcg
     7441 cgccgaccac gcttgaattt tacaagcata cagcgggcgt gttcttgaat tggactgagg
     7501 gacaaggcat cacatccccc gaacaagttg acgcgcggct ggttcggcat tatctggcag
     7561 accttacaac gaaggggaaa tcagacaaga ccttacatgc gcacgcgcga gcgattcgga
     7621 ctctgctccg cttttggcat gcagagaagt acattcccga agctgtgacc ttttccatgc
     7681 cgaagataga aaagaaacgc ctgcctgttt tatcagccga cgaactcaac aaggttttaa
     7741 cggcttgcaa gaatccgcgc gataaggctc tcattctctt tatggcggac tctggcttgc
     7801 gtagggcaga aatcaccgcc ttgaattggg gtgatgcgga cattatgagc gggcttgtga
     7861 gggtcaaacg tggcaagggt ggaaaggctc gaagcgcggt catcggggca accacccgcc
     7921 gcgccttgct tgcctatcgc cgcacattgg gaagcccgtc caatgatgcg ccgatgttca
     7981 tatcacgaag cggaggtcgg tttactggta cgggggtttt actgatattc cgaagactga
     8041 ccgagaaaac agggattcac atcacccctc acgctttacg ccgcaccttt gtaattctct
     8101 ccctgcgggc tggaatggac gttttgcacc ttcaagccat gctgggacat gcaagccttg
     8161 aaatggtgca acactacgcc caaatggtgg atgatgacct gctccaagcg catcaggcac
     8221 attcgccaat ggataacttg agacaactta cccaatagcc aacaagaaag aaccgactga
     8281 aacaagtcgg ttctttcttc aaacggctcc ctactctcca aaaatctctt tgaccctttc
     8341 cccagtaatg cctggaaata aatcctgcat catgcctgcc attcgtttat aaaaggcgcg
     8401 gtcttgatag ccttcctgcc ttgaccgata agacagacgg ttacaatgcc ggcacacgaa
     8461 atacgtgccg cctggggcaa ggtagagcac gctcacacgc cgaccacaag caaggtttgg
     8521 acaaataaac caataccgta aaccacccca aggcaaagcg gtcgtggtca aatcaatcgg
     8581 gtatttgcaa tccacctttt ctttgcttgc tctggttatg gtgta
//
LOCUS       DAOQJC010000151         8567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_4428_length_8567_cov_10.449953, whole genome shotgun sequence.
ACCESSION   DAOQJC010000151 DAOQJC010000000
VERSION     DAOQJC010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4428_length_8567_cov_10.449953"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..537
                     /locus_tag="PLE39_16865"
     CDS             235..537
                     /locus_tag="PLE39_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70057.1"
                     /translation="MAKKIDGVIEAVRYKNGQVVLVRAYERRGATFSDRVLIERKTLL
                     ERLKKGQQYIVGSREELRASTFKNGKPVLVVTQDGNDLLTTNAAATRDELEETPVF"
     gene            550..1461
                     /locus_tag="PLE39_16870"
     CDS             550..1461
                     /locus_tag="PLE39_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70058.1"
                     /translation="MKIIDKTPLQDASGNINPIARVQGTLKYGLSWFAELEAQKAVIA
                     QLDRLLEKGFVLIRNFTLPNSEIVIPIILIGAGGIWVIYVTKEKGNFEAKGDQWNTLD
                     NNGRAQPAPINFISRVSQLTTAFQKYLKIQKIEIPNPIEAVLIAADPGAQIESVRPIA
                     RVVRSDAIRQFTSTLVQARPVWRTDFIHELADRIIDPTPPAELEPVKAGSPESDLRSQ
                     GGFDEAGQDFNSGSLGFDFEDNSENPTLQIPNNAGQPSSAPRPRPAPAKKGLLGLGNT
                     QVILLAGMLLVECCVLIGFGVLLYTSL"
     gene            1468..2199
                     /locus_tag="PLE39_16875"
     CDS             1468..2199
                     /locus_tag="PLE39_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE70059.1"
                     /translation="MTTPFLSIIIPAYNEEGRLPRTLERVFAFLEKQPYASEIIVVEN
                     GSSDRTLEVAGEFSKRHTNMQVIREEKRGKGNAVRRGMLEARGEYRFICDADLSMPIE
                     EVQKFLPPAIVDFDLAIGSREAPGAIRYNEPSYRHWGGRAINLLIRILILPGLNDTQC
                     GFKCFRADVVDDLFPRQTLMGWSFDIELLYLARRKRLRIKEIPIHWYFDPDSKVNAVR
                     DALRMIGDIFQIHLNAARGRYDLKP"
     gene            2183..2800
                     /locus_tag="PLE39_16880"
     CDS             2183..2800
                     /locus_tag="PLE39_16880"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNE70060.1"
                     /translation="MISSPDLSHEQELWPAYRYIAGMDEAGRGALAGPVCVGAVILPD
                     DNPNLTQTLSGVHDSKQLTPRKRIHLIPHIKETALTWGIGFASAAEIDELGIVPATRL
                     AASRAIESLAIFPDFLLTDFRLELPELGVSQAALVKGDQRSLSIAAASILAKTARDEL
                     MRELDAQYPNYRLGIHKGYGTLSHRRAIGQFGFSPIHRKTFQIKK"
     gene            complement(2846..3685)
                     /locus_tag="PLE39_16885"
     CDS             complement(2846..3685)
                     /locus_tag="PLE39_16885"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNE70061.1"
                     /translation="MNPKRLFILFSLLGMVLFGLWLPANAAPNVQQTDPTATALIDGR
                     ILYIVKAGDNCFRVAALHGITVEQLRQLNKELDENCSLTEGQELLISIVSIVGTPTAG
                     PSPTPAPPTVTPTPFTGTTEICVLLFEDTNGDALRQETEPAIAGGAVSVTENNGEYSA
                     AQETFIPADPDVYQGICFLDVPEGNYNITVGIPDNYNPTVDLNYSLDVPAGARAFVAF
                     GAQSRDVVVDPGAEDANESTTSPVFGIIGALLLLGGAGLGYYAWRSGKPESKLAGGNN
                     ILK"
     gene            complement(3764..4183)
                     /locus_tag="PLE39_16890"
     CDS             complement(3764..4183)
                     /locus_tag="PLE39_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70062.1"
                     /translation="MPNQPLFAGLVVDEDGHPAESSFIGSEPCYVVNDAGFRRHVPAE
                     QIDRAVLAQMAEMMKGSEDILSEQTAKMLGQEDPFSKAIIEQQLKNIDKQFDTLMQAG
                     IPEDMRTYLGMMGFKVVINYHGELVRVEQPGSAGDGE"
     gene            complement(4190..4765)
                     /locus_tag="PLE39_16895"
     CDS             complement(4190..4765)
                     /locus_tag="PLE39_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70063.1"
                     /translation="MNSAFQFNQYLLKRQVFALAGKFRFFDPGGRLVLYSEQKMFKIR
                     EDIRVYSDESKAQEVLMIKARQIIDFSAAYDVVDSATGQKVGAFRRKGLASIFRDEWE
                     ILDVGDNLIGKLFEDSMGMALLRRILSNLIPQNYDIIIGTNRVADLKQNFNPFTYELN
                     MDFSMDIGRLLDRRMGLAAGILLAAVEGRQG"
     gene            complement(4790..5815)
                     /locus_tag="PLE39_16900"
     CDS             complement(4790..5815)
                     /locus_tag="PLE39_16900"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNE70064.1"
                     /translation="MMNKLNLSRRDFLKLSASGALGLALSEMGIDRALAAPPSSQGRI
                     TYSGVPLYDAPSFQAKQIHLYGIDLVVELQAEVEGDEGNPFNKAWYQVDGGYTYSGWL
                     QPVETKLQKPVYDIPAEGQLGEIVVPMSETRLIPFTYAKNGPRLYYGSTHWVKKVVVT
                     REEKSIWYEIYDSYVKKSFYVPHYNLRLIPSEELTLLSPDVPESEKLIHVDLGTQMVT
                     AFEGNTMVFQSRCSSGGKGTRTPTGEFLTYHKGPSIHMTNQDEEGVKNYYDLPGVPWC
                     TFFTGYGNAFHGTYWHNDYGRPRSHGCVNLPSEDAKWVYRWSRPNLSPNEDYLHVPGE
                     GTRVQIV"
     gene            complement(5848..6867)
                     /locus_tag="PLE39_16905"
     CDS             complement(5848..6867)
                     /locus_tag="PLE39_16905"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNE70065.1"
                     /translation="MKSSKLSRRDFLKLGGLGLAGLLTPPLNFHFDDPFAAVQGRVTT
                     RMIWMYDKPSTEATQVTFCHRDKLLNITNAVISDDVNSHNRVWYEIGKEGYVYSGNIQ
                     PVRTLLNKPQLNIPKDGMLTEISVPYTDAYEMPDKTSKVAYRMYYETTHWVKAVVTAE
                     NGQVWYQVRDDKFDKLYYARSEHMRPMSEAEIAPISPEVENKKIQVQLDEQILVAYEN
                     NLPVFVASVSTGGIFRVGTYTTPQGAFITYYKRPSRHMAAGDIAASGFDLPGVPWVQY
                     ITEGGISFHGTFWHNDFGRPRSHGCINLSTSTAKWLYRWSAPQVTFTKEFSFGGVGTD
                     VEIVL"
     gene            complement(7023..7997)
                     /locus_tag="PLE39_16910"
     CDS             complement(7023..7997)
                     /locus_tag="PLE39_16910"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNE70066.1"
                     /translation="MNNRLPTNNSNGRPSPEKQNQAKVQKKKRGPLLPILLVLMGCAV
                     FAFAAWSAINSPVLASLLNSAPAPQQAQPVQRPFAQVSISKPSAVVVQQPLIQVAMPT
                     ATLPALPVENTAQQPQPQVIDPAPTADLALNAAALPTLQVSVLPGATDAPAVEQQPAA
                     EQAPVVQEANSQPGVISAEIVVDTPTPEYVAPTEVAAAPVPVGVDTGGEHWIDVDLSQ
                     QRVYAYAGDTVVNSFVVSTGTWQTPTVTGKYKVWVKLRSAAMSGPGYYLPDVPYIMYF
                     YGSYGLHGTYWHNNFGTPMSHGCVNLTIPDAEWLYNFSSVGTVVNVHY"
     gene            complement(8131..>8567)
                     /locus_tag="PLE39_16915"
     CDS             complement(8131..>8567)
                     /locus_tag="PLE39_16915"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNE70067.1"
                     /translation="IDYPAPASQPMEISAPGYGGNKYILVDISEQHMYVYEGDALVYS
                     FVSSTGMNNATRVGSFSVLDKIPNAYGATWDIWMPNWLGIYWAGSLENGIHALPILSN
                     GATLWSGYLGIPISYGCVVLGSYESQLLYDWADVGTPVDIQW"
BASE COUNT         1944 a         2166 c         2412 g         2045 t
ORIGIN      
        1 atcctatcgc cccgacttta taaggatggc tttgtaagga ttaggcgcaa gttccccatc
       61 gagggacgat gcgaatccta ttgtcccgac tttataagga tggctttgta aggattaggc
      121 gtgggtttcc cataaaaaag cagagacacg gaggacacgg aggataactc tctgtgactt
      181 tgtgtctctg taaatttaac ccgcgtgtgt taaaattact caaaaggaat tcccatggca
      241 aagaaaattg atggtgtgat cgaagcagtt cgttataaaa atgggcaggt tgtgcttgtg
      301 cgtgcctatg aacggcgcgg tgcgacgttt tctgaccgtg tattgattga gcggaagacc
      361 ctgctggaac gtttgaagaa gggtcagcag tatattgtcg gctcacgtga agaattgcgt
      421 gccagtacgt tcaagaatgg caagccggtt ctggttgtga cacaggacgg aaacgacctg
      481 ttaaccacaa atgccgccgc cacgcgtgat gaacttgagg aaacacccgt tttctaaaag
      541 tggatttcaa tgaagatcat tgataagacc ccgttacaag atgccagcgg caatatcaac
      601 cccattgccc gtgttcaggg aaccctgaaa tacggcttga gttggtttgc tgaattggaa
      661 gcccaaaagg cggtgatcgc ccaattggat cgcctgcttg aaaaaggctt cgtgttgatt
      721 cgcaatttca ccctgccgaa cagcgaaatt gtgattccga tcattttgat cggcgcgggc
      781 ggcatttggg tcatttatgt gacgaaagaa aaaggcaatt tcgaagcgaa gggtgatcaa
      841 tggaacaccc tggataacaa cggccgggca cagcctgctc ccatcaactt tatcagccgt
      901 gtttcgcaat tgaccacagc ttttcaaaaa tatctgaaaa tccaaaagat cgaaattccc
      961 aatccgatcg aagccgtgtt gatcgcggcg gaccccggtg cccagatcga gtcggtacgg
     1021 cccattgcgc gggtggtgcg cagcgatgcg atccgacaat tcacatccac gcttgtgcag
     1081 gcgcggcctg tctggcgcac cgacttcatc cacgaactgg ctgaccgtat tattgatccc
     1141 accccgcctg ctgaattgga acctgtcaaa gcaggctctc ccgaatcgga tctgcgttca
     1201 caaggcggtt tcgatgaagc tgggcaggac tttaattcag gcagccttgg gtttgatttt
     1261 gaagacaact ccgaaaatcc caccctgcag attccaaaca atgccggtca accttcttca
     1321 gcaccgcgcc cacggcctgc ccctgccaaa aaaggtttgc tgggcctggg aaatacacag
     1381 gtcatccttt tggcgggaat gctgctcgtg gaatgttgtg tcttgatcgg ctttggtgtt
     1441 cttttgtaca ccagcctcta aattactttg acgactccat ttttatccat catcatcccg
     1501 gcctataacg aagagggccg cctgcctcgt acgctggagc gcgtgtttgc atttcttgaa
     1561 aaacaaccct acgcctccga aatcatcgtc gtcgaaaatg gcagttcaga ccgtaccctt
     1621 gaagtggcgg gggaattttc gaaacgccat accaacatgc aggtcatccg cgaagagaag
     1681 cgcggcaaag gcaatgcggt caggcgtggc atgttggaag cccggggtga gtatcgcttc
     1741 atttgtgatg ccgatctttc catgcccatt gaggaagtgc aaaaatttct cccgcccgcc
     1801 atcgttgatt tcgaccttgc catcggttca cgcgaagctc cgggggccat ccgctacaat
     1861 gagccatcct accgtcactg gggcggacgt gccatcaact tgctcatccg tattcttatc
     1921 ctgccggggt taaacgacac ccagtgcggt ttcaaatgtt ttcgcgccga tgttgttgat
     1981 gacctgtttc cgcgccagac cctgatgggc tggtcgttcg acatcgaact cctttacctt
     2041 gcccgtcgaa aacgactgcg catcaaggaa attcccatcc actggtattt tgatcccgat
     2101 agcaaggtca atgccgtacg cgatgccctg cgcatgatcg gcgatatttt ccaaatccac
     2161 ttgaacgcag cgcgcggccg ctatgatctc aagccctgac ctttcgcatg aacaggaact
     2221 ctggcctgcc tatcgataca ttgctggaat ggatgaagcc gggcgcggtg cccttgctgg
     2281 accggtgtgt gtaggggcgg tgatcctgcc tgatgacaac ccgaatctga ctcaaacctt
     2341 gagcggggtg catgattcca aacagttgac tccccgcaaa agaatccacc taattccaca
     2401 catcaaagag acagccctca cctggggcat tggttttgcc tccgctgctg agatcgatga
     2461 actgggcatt gtccctgcta cgcggttggc cgccagccga gccattgaat ccctcgcgat
     2521 cttccccgat tttctgctga ccgattttcg cctcgaactg cccgaactgg gcgtttcaca
     2581 agcagcgctc gtcaaaggtg accagcgttc tttgagcatt gcggccgcat ccatccttgc
     2641 aaagaccgcc cgcgatgaat tgatgcgcga acttgatgcc caatatccca actacaggct
     2701 gggaatccac aagggatatg gcaccctctc tcatcgcaga gcgatcgggc aattcggttt
     2761 ttctcccatc caccgcaaga catttcagat aaaaaaataa agtccaaggt caccagacct
     2821 tggactttat tgatcgaacg ggggattatt tcaaaatatt atttccgcca gccagtttac
     2881 tttcgggctt gcctgatctc catgcatagt atcccaggcc cgcaccgcct aacaggagca
     2941 gtgccccgat aatgccaaat acaggagatg tcgtcgattc gttggcatct tccgcacccg
     3001 ggtccaccac cacatcccgc gattgcgcac cgaacgccac gaaagcacgg gcaccggcag
     3061 gcacatcaag cgagtagttc aggtccacgg tgggattgta gttatccgga atacccacgg
     3121 tgatgttgta attgccttcg gggacatcca aaaagcaaat gccctgatac acatcaggat
     3181 cggcagggat gaaggtctcc tgcgccgcag aatattcgcc gttattttcc gtcacactga
     3241 ccgcgccccc ggcaatggca ggttcggtct cctggcggag ggcatctccg ttggtatctt
     3301 caaagagcag aacgcatatt tcagttgtgc cagtgaacgg tgtgggtgtg accgtgggcg
     3361 gggcaggcgt gggggagggg ccagcggtcg gtgtgccaac aattgacacg atgctgatta
     3421 acaattcctg cccttcggtc aggctgcagt tctcatccaa ctccttgttt aactggcgga
     3481 gttgctccac ggtaatgcca tgcaaggctg cgacacggaa acagttatca cctgctttga
     3541 caatgtacaa aatacggcca tcgatcaagg cggtggcggt tggatcggtc tgttgcacat
     3601 tgggtgcggc atttgcaggc agccacaacc cgaacaacac catgcccagc aatgaaaata
     3661 agatgaataa gcgctttgga ttcatacgca aaattatatc agggtttatt taacgcaaaa
     3721 acttcggagg atatcgcatc ctccgaagtt tggaaaagag actttattcc ccgtcgcctg
     3781 cgctgccggg ttgttccacc cgcaccaatt caccgtgata gttgatgacc accttgaatc
     3841 ccatcatgcc gaggtaggta cgcatgtctt cggggatgcc cgcctgcatg agggtgtcga
     3901 attgtttatc tatattcttc aattgctgtt caatgatggc ctttgagaag ggatcttcct
     3961 gcccgagcat tttggcggtc tgctctgaaa ggatatcttc actacctttc atcatttcag
     4021 ccatctgtgc gagtacggcg cggtcgatct gctcagcagg gacatggcgg cggaaacccg
     4081 catcattgac cacgtagcag ggttcgctgc caatgaacga gctttctgcg ggatgaccat
     4141 cttcatctac aacaagaccg gcaaagagag gttgattggg catgagagct taaccctgcc
     4201 tgccttccac tgccgcaagc agaatgcccg cagccagccc catgcggcgg tccagcaggc
     4261 ggccaatatc catgctgaaa tccatgttca actcataagt gaagggattg aaattttgtt
     4321 tgaggtcggc tacacggttc gtgccaatga tgatgtcgta attttgcggg atgaggttgc
     4381 tgagaatacg acgtaacaat gccatgccca tgctgtcttc aaagagtttg ccgatgaggt
     4441 tgtcgcctac atccaggatt tcccactcat cgcggaaaat ggatgcaagc cctttgcggc
     4501 ggaatgcccc gaccttttgt ccggtggcgc tatccactac atcataggcg gcggagaaat
     4561 ctatgatctg gcgggctttg atcatcaaca cctcctgcgc cttgctttca tctgaataaa
     4621 cgcggatatc ttcacggatc ttgaacatct tttgctcgct gtacaacacc agcctgccgc
     4681 ctgggtcgaa gaagcggaat ttacccgcca gcgcaaagac ctgacgctta agcaggtatt
     4741 gattgaattg gaatgcggaa ttcataatta gctcctggtg gcaaaaaaac taaacgattt
     4801 gaacacgggt accttcgcct ggtacgtgca ggtaatcttc attgggcgat aggttggggc
     4861 ggctccagcg atagacccat tttgcatctt ccgagggcag gttcacacag ccatggctgc
     4921 gcggacgacc ataatcgttg tgccagtagg ttccgtggaa ggcgttgccg tagccggtga
     4981 agaaggtaca ccacggtacg ccgggcaggt cgtagtaatt cttcacgcct tcttcatcct
     5041 gattggtcat atggatggaa gggcccttgt gataggtgag gaattcaccc gttggggtgc
     5101 gggtgccctt gccgccgctg gaacagcgtg attgaaaaac catggtgttg ccttcaaagg
     5161 cggtgaccat ctgtgtgccc aggtctacgt ggatgagctt ttcagattcg ggtacatccg
     5221 gtgagagcag ggtcagttct tcactgggga tcaggcgcag gttgtagtgc ggaacgtaga
     5281 aggatttctt gacatagcta tcgtaaatct cgtaccagat gcttttttct tcacgtgtca
     5341 caacgacctt tttgacccaa tgggtgctgc cataatacag ccgcggtccg ttctttgcat
     5401 acgtaaatgg aatcaatctg gtttcactca tcggcacgac aatttcgcca agctgacctt
     5461 ctgcggggat atcgtacacg ggcttctgaa gtttggtttc cacgggttga agccagcctg
     5521 agtaggtata cccgccatcc acttgatacc aggccttatt gaagggattg ccttcatcgc
     5581 cttccacttc ggcttgcagt tcaaccacca ggtcaatgcc gtagaggtgt atctgctttg
     5641 cttgaaaaga aggggcatcg tagagcggaa cgccgctgta ggtgatgcgt ccctgtgagg
     5701 agggaggggc ggctaaagca cggtcaatgc ccatttcgga gagagcaagc ccgagtgcgc
     5761 cagaggcgga gagtttcaaa aagtcgcggc gggagaggtt aagtttattc atcatgattt
     5821 tttgcagacg gttttccgat cagaatctta caggacgatt tccacatcgg tgcctacgcc
     5881 gccgaaggaa aattccttgg tgaaggtgac ctgcggtgca gaccagcggt aaagccattt
     5941 ggcagtggat gtggaaaggt tgatgcagcc atgcgagcgc gggcggccaa agtcattgtg
     6001 ccagaaggtg ccgtggaagg atatgccccc ttcggtgatg tattgcaccc agggcacacc
     6061 gggcaggtcg aacccgctgg cggcgatatc gccggcggcc atgtggcggg aggggcgttt
     6121 gtagtaggtg ataaatgcgc cctgcggcgt ggtgtaggtg cccacacgga agatgccgcc
     6181 cgtggatacc gaggcgacaa agacgggcag gttgttttcg taggcaacga gaatctgctc
     6241 gtcgagctga acttgtatct ttttgttttc cacctctggt gagatggggg cgatctcggc
     6301 ttcgctcatg gggcgcatgt gctcggagcg ggcataatac agcttgtcga atttatcgtc
     6361 gcggacctga taccagactt gtccattttc ggcggtgacg acggccttga cccagtgggt
     6421 ggtttcataa tacatgcggt aggccacttt ggaggtttta tcgggcattt cgtaggcatc
     6481 ggtgtagggt acgctgatct cggtgagcat gccatctttg ggaatgttca actggggttt
     6541 gttcagcagg gtgcgcacgg gttgaatatt gcccgaatac acgtagcctt ccttgccgat
     6601 ctcgtaccat acgcggttgt gcgagttgac atcgtcactg atgacggcgt tggtgatgtt
     6661 gagcagttta tcgcggtggc aaaacgtgac ttgtgtggcc tcggtggaag gtttgtcgta
     6721 catccagatc atgcgcgtgg tgacacgccc ctgcacagcg gcaaagggat cgtcaaaatg
     6781 aaaattgagc ggaggggtca gcaagccagc aagacccaat ccgcccagtt tcagaaaatc
     6841 acgtcgcgat aatttggatg acttcatttt ctttgaattt tttacaagga atttataaat
     6901 gagtccacgc aggaaccttt tctaaccgcg aaggccgcaa agaacgcgaa gaaaattcaa
     6961 aagcggttaa atctttgggg cggccttcgc atcccaaaaa tccgtttttg ggaatttggg
     7021 ggttaataat gcacattcac cacagtcccc actgaagaga agttatacaa ccactcggca
     7081 tcggggatgg tgaggttgac acagccatga ctcatgggtg tgccgaaatt gttatgccag
     7141 tatgtgccat gcaaaccata actgccatag aaatacatga tgtagggcac atcgggcagg
     7201 tagtacccag ggccagacat ggccgcagag cgcaatttga cccagacctt gtatttacct
     7261 gtcacagtgg gggtttgcca ggtgcctgtc gaaaccacga aggaattgac cacggtgtca
     7321 cctgcatagg catacacacg ctgctgcgaa aggtccacat cgatccagtg ttcgccgccg
     7381 gtatccacac cgacggggac gggagccgcc gccacttcgg ttggggcaac gtactcgggt
     7441 gtgggggtat ccaccacgat ctcggcagag atgaccccgg gctgactgtt cgcttcttga
     7501 accacggggg cttgttcagc cgcgggttgc tgctcgacgg ctggggcatc ggtggcgcct
     7561 ggcagcacag agacttgcag tgtgggcagg gcggccgcgt tgagggccag gtctgctgtg
     7621 ggggcggggt caatgacctg tggttggggc tgctgggctg tgttttccac aggcagggcc
     7681 ggcagggtgg cggtgggcat ggctacctga atcaatggct gttgcaccac aacggcagaa
     7741 ggtttggaaa tgctcacctg cgcgaaaggc cgctgtacgg gttgggcctg ttgcggggcg
     7801 ggggcgctgt tcaacaggga tgccagcacc ggtgagttga tcgctgacca ggccgcaaag
     7861 gcgaagaccg cgcaacccat taaaacaagc aggatgggga gcaaaggtcc gcgcttcttt
     7921 ttctggactt tggcctgatt ctgtttttcc gggctgggtc tgccatttga gttgttggtg
     7981 ggtaagcggt tgttcatggt ggagatgcct ttcggtagtc agtaatcggt agtcagtaat
     8041 cagtaattgg taatcagtga tcagtggata gtaatcagta ggcagtggtg atggaattat
     8101 acgccttgga cgtttgctgt gattcgtttt ttaccattga atatccacag gggtgccgac
     8161 atcggcccag tcgtagagga gctgcgattc gtagctgccg agcaccacac agccatagga
     8221 gatggggatg ccaaggtagc cagaccacag ggttgcgccg ttcgagagga tcggcagggc
     8281 gtggattccg ttttcgaggc tgcccgccca gtaaatgccg agccagttgg gcatccagat
     8341 atcccaggtg gcgccgtagg cgttgggaat tttatcgagc acgctgaagg agccgacgcg
     8401 ggtggcgttg ttcatgcctg tggatgagac aaagctgtac accagcgcgt cgccttcata
     8461 gacgtacatg tgctgttctg agatgtcgac gaggatgtat ttgttcccac catatcccgg
     8521 ggcggagatt tccatcggtt gtgaggcagg tgcggggtag tcgatga
//
LOCUS       DAOQJC010000152         8533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_4456_length_8533_cov_10.440080, whole genome shotgun sequence.
ACCESSION   DAOQJC010000152 DAOQJC010000000
VERSION     DAOQJC010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4456_length_8533_cov_10.440080"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLE39_16920"
     CDS             <1..213
                     /locus_tag="PLE39_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNE70068.1"
                     /translation="YYPQIMKFNLSDYKVRILDSDHGTEAITRVLIDTRNSNSRWSTV
                     GAGTNIIEASWRALADSVEYGLMVAH"
     gene            277..1335
                     /gene="leuB"
                     /locus_tag="PLE39_16925"
     CDS             277..1335
                     /gene="leuB"
                     /locus_tag="PLE39_16925"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNE70069.1"
                     /translation="MNFKITLLPGDGIGPEVVGEAVRVLETIASKYKHSFQFQERLMG
                     GCSIDKYGSSLTDETLADCQASDSVLLGAVGGPKWDDPKAKDRPERGLLALRKGLGVF
                     ANLRPVKVHPALMEWSPLKPEKLQGVDILVVRELTGGLYFGFPKGRDIKDGRERAVDT
                     LEYYDYEVKRVMELAFNLARGRKKKVTSVDKANVLESSRLWRQIAVKVGEANKDIELE
                     HTLVDTASMRLITGPAWMDVVVTENMFGDILTDEASVLAGSMGMLPSASLGESTVGLY
                     EPIHGSAPDIAGKGIANPIGTILSTAMMLRHSFKLESEAAAIEKAVDETVTAGARTAD
                     IGGKLTTRQMADEIINRL"
     gene            1410..2324
                     /locus_tag="PLE39_16930"
     CDS             1410..2324
                     /locus_tag="PLE39_16930"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNE70070.1"
                     /translation="MESKFIWMNGELVPFEKATLHFLTPALHYGAAVFEGIRAYDTPK
                     GPAVFRLTDHAERLLDSARVFGFRDLPWTTGQIVDAVKQTVKANNFKDCYIRPLLYLD
                     GGGWNLNVDYGKASLAIAAWEWGNYLGEEARAKGIRANISSFTRHHPNVLMTKAKVAG
                     NYANSFLAKTESERLGFQEAIMLDPQGYVAECTGENLFMVRHGKIVTPSTAPVLEGVT
                     RHSIHTIAKDMGFRILEQPISRDQLYIADEVFVCGTAAECIGLSEIDFRKIGDGMTGP
                     VTKRIQDTYHEAIRGKLPQYEAWCEYVD"
     gene            2473..4044
                     /locus_tag="PLE39_16935"
     CDS             2473..4044
                     /locus_tag="PLE39_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70071.1"
                     /translation="MLDTVRPYITKDEPFGPIDSTDIAPGDIKALSLLFDQHNWIYKN
                     LRTRPSIIIGRRGSGKTSYLRSVFFDKQYDHYTEIRTARVLGHMAKVVQKATKEAFFP
                     EMVSELWEMILWVAVFVEVRKHSLLSSDEMYVVNAYLEKLDVRGVDDIDSLFWDLANT
                     LDAALEKNPSRGALDILRGLDRFTFDYAKTIVLESFERSKTSFVVLMDSLDDFQLDID
                     SVAHSLQGLLKLVGSMNKPRDMVDIRFCLPAEVYHRFTAISSNPNKDFRRALRLQWSA
                     SELIFIGARRLGFYLDIFYPDKLRALYPLNVSNRADALRLFQSVLPDRLENQAGFQED
                     TMSYILRHTQLLPRHLLMLLNSIFRKTGVTQTLTPFPVPKERIINGIRQVEELIVKEI
                     FVAFKLIYPTAEETCRRCLPELGHKFTMGDLHRVFTRHGKAVFGGENLFEFQRMLIEI
                     GAIGRVIPGKESDLYIKGNFEYTVQHQLALGQDDEICVHPLFSGIFQNNGKSGRPVYP
                     YGSVLEDADYRDVED"
     gene            4179..5390
                     /locus_tag="PLE39_16940"
     CDS             4179..5390
                     /locus_tag="PLE39_16940"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNE70072.1"
                     /translation="MKWIKSHPWLTGLLVVLAACLVFFFYTDLHLLYRQRPTEFEAAI
                     TQDLADAKIPGAALLFMRDGQVESSKGYGLADVESGRGVDADTLFTVASVSKPVTATA
                     LMTLYEQGKFELDDDINDYLPFEVRNPTFADTPITFRMLLTHTSSIVDSSAYERFYTL
                     KKEPVLPDSPISLEEFVTSYFDPQGEWYDTEENFLKEAPGVAYYYSNMGYGLIGYLVE
                     QISGMPFNEYCKQAIFEPLGMEHSAWFFKDVDVNQVAVPYGYDDLRREPKPFGFYGYP
                     TYPDGALKTSVNEYARFLSVFINEGRTLEGGQFLKPETVAEMLRTQSLLGIEAPAAVG
                     FAWHYDGESYLHTGGDPGISTLVYFNPEADRAVIFFTNGGGEYDVPGVLRLIFVDSKL
                     SPMLLEKMNAP"
     gene            5412..6854
                     /locus_tag="PLE39_16945"
     CDS             5412..6854
                     /locus_tag="PLE39_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70073.1"
                     /translation="MKRNILLLAFILIVMAAIVFVLPALVKDVFSNTISAQARRAVIQ
                     TFTIIVVCVSGIVYFSSGAHKREESGMWVTAITLSVFCLTGIGLIWYMVSRSSSNENS
                     PAYYHIITPSSVSGVCEKIDLSALDSQPSKGNPINVRFIDAKAEVDCLMKEQSDLASH
                     TKGEAPIYIPTGVFIQSLKFDSANDVILTGFVWQRYANDVPAEIERGFVIPEGDSEIF
                     VREDTAVYKKQQGNETLYGWYINISLRQVFDYSHYPFDRQDIWLRLWHLNFDRNVILV
                     PDFSAYHLEAVWPQDGKTFPGIERDFVLERLSLERTYFSYHYNHYNTNFGIDSYDAHT
                     VFPELYFNVGVRRDVLDAFVAYMIPMFILILMSFGVQFIVTQDPEKMTMHGISTTGLI
                     AYYASLFFIGILAQLDIRRTLNAPGVVYIEYFYFVLYIILLLQTVNSVVFAASDRVEW
                     LEYKDNLIPKVVFFPVTLGLMYLITAFLFY"
     gene            complement(6968..7672)
                     /locus_tag="PLE39_16950"
     CDS             complement(6968..7672)
                     /locus_tag="PLE39_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70074.1"
                     /translation="MNQTIEKAIDAYGGREFWLKAKRIEAEVSTHGLAFTIKSRPTFK
                     RAKISMEIARPFSKLMPIGKITQLAGVLDEHHVRLEDSTGRIVEERKDARKYFPGGLQ
                     RLFHWDDLDMAYFANYAFWNYFTLPVLLMREDIDWKEIGPGWLEATFPESIPTHSKIQ
                     RFRFDLKTGLLIQHDYTVDIMSKLATAANVVLEHKQGAGFLYPSRRLVTPRSPSGKPL
                     PAPTLIDITVHEFQVK"
     gene            complement(7669..>8533)
                     /locus_tag="PLE39_16955"
     CDS             complement(7669..>8533)
                     /locus_tag="PLE39_16955"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE70075.1"
                     /translation="MTNKTRPSWLNEKEYPFKDHYFKTPQGDMHYVDEGTGDPIVLLH
                     GNPGWSFEYREIIKEMSKTQRCIAPDYIGFGLSDKPADWDYLPINHAKHVEAFLDSLN
                     LNNITFVFNDWGGPPAISYAIKHPEKVKKIVVTNSWFWPVDDIPNFTRFSGMAGGPIG
                     KFFTLNFNMIGKVLSKAAYGPKKKMPEEVRKHYYMPHEEKTARVGTWVFPREIIGSTP
                     WLKTLWDQREKIQHIPTAIVWGDSDIAFKEGELKVWTSLMKNHTLNVLHRIGHYPPEE
                     APEEVIKALKA"
BASE COUNT         1997 a         2106 c         2168 g         2262 t
ORIGIN      
        1 tattacccgc agatcatgaa attcaatttg tccgattaca aagtccgcat tttggattcg
       61 gaccacggca ccgaagccat tacccgagtg ctgatcgaca cccgcaattc caactcccgc
      121 tggagcaccg ttggcgcagg cacaaatatt atcgaagcct catggcgtgc attagcagac
      181 tcagtggaat atggattgat ggtggcgcat taattaccaa ttatcaattg ctaattacgg
      241 taaaaagtaa ttggtaatta gagattacgg agaacaatga acttcaaaat caccttactc
      301 cccggcgatg gcatcggacc agaagtggtc ggcgaagccg tacgcgtgtt ggaaacaatt
      361 gccagcaaat ataagcactc atttcaattc caggaacgat tgatgggcgg ctgctcgata
      421 gacaaatacg gctcttccct gaccgatgag actctcgccg attgccaggc ctcagactcc
      481 gttttgctgg gtgctgtcgg cggaccgaaa tgggacgacc ccaaagccaa agaccgcccc
      541 gagcgcggat tgctcgccct gcgcaaaggc ctcggcgtat ttgccaacct gcgcccggtc
      601 aaagtccacc cggcgttgat ggaatggtca ccgctcaagc ctgagaaact gcaaggcgtg
      661 gatatcctcg tcgtgcgtga gctcacgggc ggtctgtact ttggcttccc caagggacgc
      721 gatatcaaag acggacgcga acgtgcggtg gatacactgg aatattacga ttacgaagta
      781 aaacgtgtta tggaacttgc cttcaacctc gcccgtggac gcaagaaaaa ggtcacctcg
      841 gtggataaag ccaatgtgct cgaatcctca cgtttgtggc ggcagatcgc tgtcaaagtg
      901 ggtgaggcga acaaggacat cgagttggaa cacactctgg tggatactgc atccatgcgt
      961 ttgatcactg gtcctgcatg gatggatgtt gtcgtcaccg agaatatgtt cggcgatatc
     1021 ctcaccgacg aggcttcggt tttggcagga tcaatgggaa tgttgccttc tgccagtttg
     1081 ggcgaatcaa ccgtcgggtt gtatgaaccg atccatgggt ctgctccaga tattgcaggc
     1141 aaaggaatcg caaatccgat cggtacgatc ctttccacgg cgatgatgct gcgtcattcg
     1201 tttaagttag aatctgaagc cgcggccatt gaaaaggcag tggatgaaac tgtgactgca
     1261 ggggcgcgca cggcagatat cggcggtaag ttaacgaccc gtcaaatggc ggatgaaatc
     1321 ataaacagac tatagacgat ggatcatgga tgacaatctg tcgtctgcgg tctgtggtcg
     1381 aacaaaaaaa ggagaattga atcatgggaa tggaatccaa atttatctgg atgaacgggg
     1441 agcttgtgcc gttcgaaaaa gcgactctgc attttctcac ccccgctttg cattatggcg
     1501 cggcggtgtt cgaagggatt cgcgcgtatg acaccccgaa gggacctgcg gtctttcgct
     1561 tgaccgacca cgcagaacgc ctgctcgatt cggcgcgtgt gttcgggttc cgtgacctgc
     1621 cgtggacgac cgggcagatc gtggatgcgg tgaagcaaac ggtgaaggcc aataatttca
     1681 aggattgtta cattcgcccg ctcttgtatc tggatggcgg cggctggaat ctgaatgtgg
     1741 attacggcaa ggcctctctt gccattgccg catgggaatg gggcaactac ctcggtgaag
     1801 aagcacgcgc aaagggaatt cgcgcgaaca tttcttcgtt cacgcgtcat cacccgaatg
     1861 tgttgatgac caaggccaaa gtggctggaa attacgcgaa cagtttcctt gccaaaaccg
     1921 aatcagagcg attgggtttc caggaagcca tcatgctcga tccgcaaggc tatgtggcgg
     1981 aatgtacagg tgagaatttg ttcatggttc gccacggcaa gatcgtcacc ccgtccaccg
     2041 cgcccgtgtt ggaaggtgtg acccgccatt cgatccacac cattgccaag gatatgggct
     2101 tccgcattct ggaacagccg atctcacgtg accagcttta cattgccgat gaggtctttg
     2161 tgtgcggaac ggcagctgaa tgcattggct tgagtgaaat tgatttccgc aagatcggtg
     2221 acggcatgac cgggcctgtg accaaacgca ttcaagatac ctatcatgaa gcgattcgcg
     2281 gaaaactccc acaatacgaa gcgtggtgcg aatacgtgga ctaggtgtga cagagatcaa
     2341 aatcgggtga agcaaaaagc ttcgcccgat tttctttttg ctttctggct atccgcctct
     2401 gttcatcgca atttcgggtt tgattgtata atccgcccag agttttcacc ccacaaggag
     2461 gaaatcccag ccatgttgga tactgtccgc ccctacatta caaaagacga gccatttgga
     2521 ccgatcgact caactgacat tgccccgggc gatatcaagg cattaagcct gcttttcgac
     2581 cagcataact ggatctacaa gaacctacgt accaggccgt ccattatcat tggccgcagg
     2641 ggttcgggga aaacctccta cttacgcagt gtctttttcg ataaacaata tgaccattac
     2701 actgagatcc gtactgcaag ggttttgggg cacatggcca aggtggtaca gaaagcaacg
     2761 aaagaagcct ttttcccgga gatggtctcc gaactttggg agatgatcct gtgggtggcc
     2821 gtcttcgtgg aagtgcggaa acattccctg ctttcttccg atgaaatgta cgtggtcaat
     2881 gcctatctcg aaaaattgga tgtccgcgga gtggatgaca ttgacagcct gttctgggac
     2941 cttgccaaca ccctcgatgc agctctggaa aaaaatccca gccgcggcgc gctcgatatt
     3001 ttgcgcggac tggaccgctt tacctttgat tacgccaaaa ccattgtttt ggaaagtttc
     3061 gaacgttcaa agacctcctt tgtcgtgttg atggattcgc tggacgactt ccaactggat
     3121 attgactcag tggcgcattc gcttcagggc cttctgaaac tggtcggctc catgaacaag
     3181 ccgcgcgata tggtggatat ccgcttctgt ttgcctgcgg aggtgtatca ccgttttaca
     3241 gcgatctcct ccaaccccaa caaagacttc cgccgcgccc tgcgcctgca atggtcggcc
     3301 agcgaattga tcttcattgg cgcgcgcagg cttggttttt accttgatat tttctacccc
     3361 gataaattgc gcgcgcttta cccgttgaac gtttccaacc gtgccgatgc cctgcgtttg
     3421 ttccaatcgg tgctgccaga tcggctcgag aatcaggctg ggtttcagga agacacgatg
     3481 tcttacatcc tcaggcatac gcaattattg ccgcgccacc tgttgatgtt gttgaattcc
     3541 atcttccgaa aaacaggtgt tacccaaacc ctcacaccat tccccgttcc caaggaacgc
     3601 atcatcaatg gcattcgtca ggtggaggag ttgatcgtca aggaaatctt tgtggctttc
     3661 aaactcatct acccaactgc cgaggaaacc tgccgccgct gcctgcccga gttgggacac
     3721 aagttcacca tgggcgacct gcaccgcgtt ttcacacgac atggcaaagc ggtctttggc
     3781 ggcgaaaatc tttttgaatt ccaaagaatg ctgatcgaaa tcggcgccat tggcagggtg
     3841 attcctggaa aagagtctga cctctacatc aaaggcaatt ttgaatatac cgttcagcat
     3901 caactcgctt tgggtcagga tgatgaaatt tgcgtccacc cactcttctc gggaattttt
     3961 caaaataacg gtaaatcggg ccgtccggtc tatccttatg gaagtgtgct cgaagatgca
     4021 gattatcgag atgtggaaga ctgacccttg aagttggact gcgactaaaa aagcgcgtcc
     4081 aggcgacgcg ctttttgctg cttctgtttt ctgacaaatt tccgcagccg aaccctttcc
     4141 gctgcgtgga caatgaactt gcaaaaaagg attttcacat gaaatggatc aaatctcacc
     4201 cctggctgac aggtctgctg gttgtgctgg cggcctgtct cgttttcttc ttctataccg
     4261 acctgcacct tctctaccgt caacgcccca cggagtttga agccgccatc actcaagacc
     4321 tggccgatgc gaagatcccc ggcgccgcgt tacttttcat gcgcgatgga caagtggaat
     4381 cttcaaaggg ctacggtctc gcagatgttg aatcaggccg gggcgtggat gctgatacgc
     4441 tttttacggt cgcatctgtc tccaagccgg tcactgccac agcgttgatg acgctttatg
     4501 aacagggtaa attcgagttg gatgatgaca tcaacgatta tctgccgttc gaagtacgca
     4561 accccacctt tgccgatacc cccatcactt tccgtatgct gcttacccac acgtccagca
     4621 ttgtggatag cagtgcctat gaacgtttct ataccttgaa gaaggaaccg gttttaccgg
     4681 attcgcccat ttcgcttgag gaatttgtca cctcttattt tgatccgcag ggtgaatggt
     4741 acgacaccga ggaaaacttc ctaaaggaag cgcccggcgt tgcctattat tacagcaaca
     4801 tgggctatgg cttgatcggt tatcttgtgg agcagatcag cggcatgccc ttcaatgaat
     4861 attgcaaaca ggcgattttt gagccgctcg gcatggaaca ttctgcctgg ttcttcaagg
     4921 atgtggacgt aaatcaggtg gcggttccat acggttatga cgatttgagg cgcgaaccaa
     4981 aaccctttgg cttttatggg tatcccacct atcctgatgg tgcattgaag accagcgtga
     5041 atgagtacgc ccgtttcctt tctgtgttta tcaacgaagg cagaacactt gaaggcggac
     5101 aatttctaaa acctgaaacg gttgcagaaa tgctcagaac gcaaagcctg ctgggtattg
     5161 aagcgcctgc cgctgtgggg tttgcctggc attatgacgg cgaatcctat cttcataccg
     5221 gcggtgaccc gggcatcagc acattggtgt atttcaaccc cgaagctgac cgtgcggtca
     5281 tcttctttac caatggcggc ggtgaatatg atgtgcccgg tgtgctccgc cttatatttg
     5341 tagattcaaa actttccccc atgttgctgg aaaaaatgaa tgctccataa gcttgatgtg
     5401 ttgaggtgct catgaaacgt aacatactgc ttctggcctt tattcttatt gtgatggcgg
     5461 cgattgtatt tgtcctgccc gcccttgtga aagatgtctt ttcgaacacc atttctgcgc
     5521 aggcacggcg ggcggtgatt caaacgttta cgatcatcgt cgtctgtgtt tcgggcattg
     5581 tttatttctc cagtggtgct cacaaacgtg aagaaagcgg aatgtgggtg acggcgatca
     5641 ctctttctgt tttctgtctg accggtatcg gcctgatctg gtacatggtc tcaagaagtt
     5701 cgtccaatga gaatagccct gcgtattacc atatcatcac gccgtccagc gtgagcggcg
     5761 tttgcgagaa aattgatctc agcgcgctcg attctcagcc gagcaagggt aacccgatca
     5821 acgtccgttt cattgatgcc aaagccgagg tggattgctt gatgaaagag caatctgacc
     5881 ttgccagtca cacgaagggg gaggcgccga tatacatccc caccggggtg tttattcaat
     5941 cactgaaatt cgacagcgcc aatgatgtga ttttgacagg atttgtctgg cagcgttatg
     6001 cgaatgatgt tcctgcagag attgaacgtg gttttgtgat acccgaaggt gattctgaga
     6061 tctttgtaag ggaagataca gcggtataca aaaaacaaca gggaaatgaa accctgtatg
     6121 gctggtatat caatatttcc ttgcgtcagg tgtttgatta ttcccattac ccatttgaca
     6181 ggcaggatat ctggctgcgc ctgtggcatc tcaattttga ccgaaatgtg attctggtcc
     6241 ctgatttttc cgcctaccac ctcgaagcgg tctggccgca ggatggaaag accttccccg
     6301 gcatcgaacg tgactttgtg ctggaacgcc tctcgttgga gcgaacctac tttagttatc
     6361 actacaatca ttacaacacc aattttggca ttgacagtta cgatgcccac accgtttttc
     6421 ctgaattgta tttcaacgta ggtgtgcgcc gcgatgtttt ggacgcattt gtggcataca
     6481 tgatccccat gtttattttg attttgatgt cttttggcgt acagtttatc gttacgcaag
     6541 accccgaaaa gatgaccatg catggaatca gcaccaccgg gttgatcgct tactatgctt
     6601 cgctcttttt catcggcatt ctggctcaac ttgatatccg ccgcacattg aatgcgcccg
     6661 gtgtcgtgta tattgaatat ttttattttg ttctttatat cattctgctt ttacaaacgg
     6721 tcaactcggt tgtctttgcc gcttctgatc gtgttgaatg gctggagtac aaagataacc
     6781 tgatcccgaa agttgttttt ttccctgtta ctctcggact gatgtattta atcacagcct
     6841 ttctgtttta ctagcctcct gaccaggcaa aaaaccgttg taagcacagt aacgcgggga
     6901 aacaccccgc gttactctat aaattccctg cagaattaat attttatact gctcaaagct
     6961 gcacttgtta tttgacttga aactcatgca ctgtaatatc gatcaaagtt ggggcgggga
     7021 ggggctttcc agatggcgag cgcggtgtga ccagtctcct tgaggggtat aagaaaccag
     7081 caccttgttt gtgctcgagg acgacgtttg cagcggttgc cagtttgctc atgatgtcta
     7141 cggtatagtc atgttgaatg agcagacccg ttttcaggtc aaagcggaag cgctgaattt
     7201 tgctgtgggt ggggatgctt tcggggaagg ttgcttcgag ccagccaggt ccgatctctt
     7261 tccagtcaat atcctcgcgc atgagcaaaa caggcagggt gaaataattc cagaaggcat
     7321 agttggcgaa atatgccata tccagatcat cccaatggaa taaccgttgc agaccgccag
     7381 ggaaatactt gcgggcatct tttcgttctt caacgatcct gccagttgaa tcttcgaggc
     7441 ggacatgatg ttcatccagc acaccagcca gttgtgtgat tttcccaata ggcatcagct
     7501 tggaaaacgg gcgggcaatc tccattgaga tttttgcgcg cttgaaggtg ggtctgcttt
     7561 ttatcgtgaa cgccaaccca tgtgtgctga cttccgcttc gatacgtttt gcttttaacc
     7621 aaaactctct gccgccataa gcgtcaatcg ctttttctat ggtttgattc atgctttcaa
     7681 tgccttgatg acttcttcag gcgcttcttc aggcgggtag tgaccgattc tgtgcaagac
     7741 attgagcgta tgatttttca tcagactggt ccacactttt agctcacctt ccttgaatgc
     7801 aatatccgag tcgccccata caatggcggt ggggatgtgc tgaatctttt cacgttgatc
     7861 ccaaagcgtc ttcagccacg gtgtggagcc gatgatctca cgcgggaaga cccatgtgcc
     7921 aacgcgcgcg gtcttttctt catggggcat gtaataatgt ttgcgcactt cttcgggcat
     7981 ctttttcttt ggtccatacg cagctttcga taagaccttg ccgatcatgt tgaagttcaa
     8041 cgtgaagaat ttgccaatgg ggccaccagc catgccgctg aatctcgtga agttgggaat
     8101 gtcatccacg ggccagaacc agctatttgt gaccacgatc tttttgacct tttcaggatg
     8161 tttgatagcg taagatattg cgggcgggcc gccccaatcg ttgaatacaa atgtgatgtt
     8221 gttcaggttg aggctgtcga gaaaagcttc cacgtgtttg gcgtggttga taggcagata
     8281 atcccaatcg gcaggcttgt ctgaaagccc aaagccgatg tagtcgggcg cgatacagcg
     8341 ctgggtcttc gacatctctt tgatgatctc gcgatactcg aatgaccaac cagggttgcc
     8401 atgcaacagt acgatcgggt cgcctgtgcc ttcgtccaca taatgcatgt cgccttgtgg
     8461 cgttttgaag taatgatcct tgaagggata ttctttttcg ttcaaccaac tgggtcttgt
     8521 tttgtttgtc att
//
LOCUS       DAOQJC010000153         8446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_4536_length_8446_cov_9.463830, whole genome shotgun sequence.
ACCESSION   DAOQJC010000153 DAOQJC010000000
VERSION     DAOQJC010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8446
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4536_length_8446_cov_9.463830"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..709)
                     /locus_tag="PLE39_16960"
     CDS             complement(143..709)
                     /locus_tag="PLE39_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70076.1"
                     /translation="MKNWENFIKSNKTFIYIGIAFVCIGLGGLFSVKFLHQNLVIGIF
                     IGAMAATLILVAIAPSIEHKFRKDSLGQQIKRPSSPLFKDLRAYLHPTRRHTTIGFLW
                     CFFNIFFIVPLFGKQSENLSTVNSISILFLLFISLDGYQMVKRNVRISSKYGYLIHGA
                     GSQYIGYLILFFCWTGCIVLILSMIIGW"
     gene            750..2072
                     /locus_tag="PLE39_16965"
     CDS             750..2072
                     /locus_tag="PLE39_16965"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNE70077.1"
                     /translation="MDIETKYNQALDYLYSFVDYSLKHSSELAKADFNLDRMFALMES
                     LGNPQKKYPIIHVAGTKGKGSTSALCASALRAAGYKVGLYTSPHLLDYTERIQVNGAP
                     ISHEQLVALVDEIRPHVSKIEKLTTFEITTALAFMAFAKYGMDAAVFEVGLGGRLDAT
                     NIVTPNVSIITSLSYDHMAVLGNTLTLIAGEKAGIIKPGIPVVSSPQKDEAFQVLERV
                     AKEKSAGLTLVGRDVTFELVESSLDGQKFNLQPSTFNHHPLSFVIPLLGAHQIENAAT
                     AYAALKISGIPITDEQIQKGFSQVQWRARFEVARREPPVIFDSAHNQDSFQKLGETLE
                     TYFPGKKVILIFGASEDKSILDMFTALKPKIQKIIVTRADHPRALEVERIQQLAGQAG
                     AESEAVTPVKSALMRALELSSNDGSIVLSAGSMFVTAEVMSAWKTRNE"
     gene            2069..4543
                     /gene="lon"
                     /locus_tag="PLE39_16970"
     CDS             2069..4543
                     /gene="lon"
                     /locus_tag="PLE39_16970"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNE70078.1"
                     /translation="MNYQSDWNENEDNFDLTLSQRTEELYRVPNMEPNADGLIEAVVL
                     PLRDMVIFPRMVSPIFVGREASLLAVREAQAKEQTVIGLTQKDPDLNDPNAEDFLPVG
                     VEMAVGRMLQMPDGSYSALVQGRKRVEIVEFLRVKPYIKVRARVINEPTSADRQTQAL
                     MRSVLSLFERCVQLDRSIPEEAHLFAMNIAEPGWLCDMIATSLSLTYEAKLRLLLVLD
                     PRARLGLLNNLLAQEVDVLELEDEIHSRVQSEVDKSQREFYLREQMKQIQNELGEGDV
                     FSRDASDLKKKLDAANLPDEVRKVAMKELERLSQMPPMAPEVGMIRTYIDWIVDLPWS
                     NSSPDNLDVKNAAKILERDHYGLKKAKDRILEYIAVRSLKPKKERQPILCFVGPPGVG
                     KTSLGRSIADALGRKFVRVSLGGVRDEAEIRGHRRTYIGALPGRIIQTMKRAGTTNPL
                     FMLDEIDKLYSDFRGDPASAMLEVLDPEQNYAFSDHYLELPFDLSKVMFVTTANSLGS
                     IPSPLLDRMEIIEFPGYIEEEKVEIANRYLIPRQLEESGVDNLGLVFEPAALQRIIRE
                     YTYEAGVRSLEREIGRVCRKVARMKAEGKKYPLAIAPELIEKLLGPQQVFPPEAERSD
                     EVGVATSLAWTETGGEIMSVEVAVLEGKGGMQMTGQMGEVMQESAQAALTYIKSRASK
                     LKIELEAFERLDIHVHMPEGGIPKDGPSAGITMATAIISALTGRPIFHHVGMTGEITL
                     RGRVLPIGGVREKVLAAHRAGLKTVLLPEKNMKDLVDLPKTAKSELKIIPIKHMDDVL
                     ALALSEKVTVEPPRPKKQARDDSQED"
     gene            4686..5495
                     /locus_tag="PLE39_16975"
     CDS             4686..5495
                     /locus_tag="PLE39_16975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE70079.1"
                     /translation="MSKLQNKVVLITGASSGFGEDAAMLFAQEGCKVVLAARRMDRLQ
                     NLAERIQSAGGEAIAIPVDIVNVADVDNMVQTTLDLYGHIDILFNNAGIGRVEWFENH
                     TLERDLDTLVHVNLTSLMQVTRSVLPHMLARGEGHIINMISVAGLLATPLITSYAASK
                     HGVRGFTDALRREVAPLGIKVSGIYPGPASTEFGQHVGKNEAYRSVRSRLKIHMSSSY
                     VARRVVDVAKRPRRSLIIPWWFRILTTIEAVFPALVDWISYSFTKKKHKLD"
     gene            5505..6611
                     /locus_tag="PLE39_16980"
     CDS             5505..6611
                     /locus_tag="PLE39_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNE70080.1"
                     /translation="MTTARLKSLIDSLPTSNLDAVILNPGPTLTHLTGVHFHLMERPV
                     VLFVAPGQDPALVLPELELPKLGLFPYKVQAFAYGENPSEWDDAFRKAAQALGLDGKR
                     IGVEPRQLRLLDFRHVKAGAPEAEYPDASEVLSRLRLKKDKAEVDAMRRAVKIAQDAL
                     EATIPLIKIGMTERELSSELVIQLFKQGSDPELPFAPIVSGGPNAANPHASPTERKLQ
                     AGDLLVVDWGAAFDGYISDLTRTFAVGEVDDECNKIHKIVQEANAAGRAAGGPGVSCA
                     NVDKATRDVIEASGYGKYFTHRTGHGIGMEGHEEPYMRGDNMQLLEPGMAYTVEPGIY
                     LTDRNGVRIEDNVVITENGAESLSDMPREIRVVG"
     gene            6618..7091
                     /locus_tag="PLE39_16985"
     CDS             6618..7091
                     /locus_tag="PLE39_16985"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE70081.1"
                     /translation="MQDPVYVICRATSTDADRLTEITIAAKRHWNYPEKWIQFWLPQL
                     TLTAEYISNHEVWMVMAKDAAIGYYSLEYSDGELWLGNLWVLPEHMGQGIGRLLFEHA
                     LNRCRGLGCSTLKIEADPNAQSFYERMGACKIDEHCSEVDNQPRILPVMEIVVPA"
     gene            7220..7993
                     /locus_tag="PLE39_16990"
     CDS             7220..7993
                     /locus_tag="PLE39_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNE70082.1"
                     /translation="MSEFTPTPERQKQILSLLGKQGRLSVAEIVEQFSISEATARRDL
                     ETLASQGKAQRVHGGVIALEQAPPELPILEREGEQTDEKASIGRAAAELIADGETVFL
                     GSGTTVLEAAKHLRDRKNLTVITNSLPVLNVLAGIKDITVVSLGGQLRESELSFIGHI
                     TEQALAKLRVDKVIMGTRGISLEHGLTNDYLPETLTDRAILKIGRTVIIAADHTKINR
                     VATALLAPLKSMQVFVTDSHAEKKFLQSLKKLEIEIVIA"
     gene            8019..>8446
                     /locus_tag="PLE39_16995"
     CDS             8019..>8446
                     /locus_tag="PLE39_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70083.1"
                     /translation="MLLKNFRPQSKLVTKTTLVNKPKFPVIDAHDHLGEPFGGGWDKK
                     PLSELLDLLDEAGVTHYVDLDGGWGEDILDAHLKHFKEGAPERFQVFGGVDWNKWQEL
                     GNQFPEWAAKRLIAQKERGAQGLKIWKPLGLHVKDHQGKLV"
BASE COUNT         2071 a         2099 c         2288 g         1988 t
ORIGIN      
        1 attcacggtg caaacgagag agcagcggct tgattcgtcc gcgcttttct ttgagcgagg
       61 tacataaggg cagtcgaagg tggatggtga ggatagcgag catggtttga tttgcgattt
      121 gagattttgg atttacgatg tattaccagc caattatcat tgaaagaata aggacaatac
      181 atcctgtcca acagaaaaat aaaattaaat agccgatata ctgtgacccg gcgccatgga
      241 ttaaatagcc gtactttgag ctgattctta cgtttctttt gaccatttga tagccgtcca
      301 ggcttataaa caataaaaac aaaatagaaa ttgaattgac ggtggataag ttttcgcttt
      361 gttttccaaa gaggggaaca atgaagaaaa tgttgaaaaa acaccataaa aatccgatcg
      421 tggtatggcg tctggttgga tgtaagtagg ctcttaagtc cttgaataat ggacttgatg
      481 gccgtttgat ttgctgacca agcgagtcct ttctaaattt gtgttcaata ctcggggcta
      541 ttgcaacgag gatcaatgtg gcggccattg ctccaatgaa tattccgatt accagatttt
      601 gatgtaggaa tttcactgaa aaaagcccgc ccagaccaat acaaacaaag gctatgccaa
      661 tatagatgaa tgtcttgttg ctttttatga aattttccca gtttttcatt tactagacct
      721 gtctgtttta ttagactaca atcccgcaca tggatattga aaccaaatac aatcaagcgc
      781 tcgattatct ttattccttt gtggattata gcctgaaaca ttcctccgaa ttggcgaagg
      841 ctgatttcaa ccttgaccgc atgtttgcgt tgatggagtc tttggggaat ccgcaaaaga
      901 aatatcccat catccatgtg gcagggacga agggaaaggg ttccacttcg gcgctttgcg
      961 cttcggcgtt gagagcggcg gggtacaagg tcggcttgta cacgtcaccg catttgttgg
     1021 attacaccga gcggattcag gtgaacggcg cgccgatctc gcatgagcaa ctggttgcgt
     1081 tggtggatga gatccgcccc cacgtgtcaa aaattgaaaa attgaccacc tttgagatca
     1141 ccaccgcatt agccttcatg gcttttgcga aatatggcat ggatgcggca gtgtttgaag
     1201 tagggttggg tggacgcttg gacgccacca atattgtgac gccgaatgtt tcgattatca
     1261 cttcattatc gtacgatcac atggctgtac tgggaaatac cctgacgctg atcgcggggg
     1321 agaaggctgg catcatcaaa cctggcatcc ccgtggtttc ctcgcctcaa aaggacgagg
     1381 ctttccaagt cttggagcgt gtagccaaag aaaagagtgc tgggctaacc ctcgttggca
     1441 gagatgtaac attcgagttg gtcgagtcat ccttggatgg gcagaagttc aacctgcaac
     1501 cttcaacttt caaccatcat cctttatcct tcgttatccc cttgcttggc gctcatcaga
     1561 tcgagaacgc agccacggct tatgccgcac tcaagatcag cggaattccc attacagatg
     1621 agcagataca aaaaggcttt tctcaagtac aatggcgcgc ccgcttcgaa gtggcgcggc
     1681 gtgaaccgcc tgtgatcttc gattcagcgc acaaccagga ttcatttcaa aagttgggcg
     1741 aaacgcttga aacgtacttc cccggtaaga aggtgatcct gatcttcggc gcatcggaag
     1801 ataaaagcat tctcgatatg tttaccgcct taaagccgaa gattcaaaag atcattgtca
     1861 cgcgtgccga ccatccgcgg gcgcttgagg tggagcgaat tcaacagttg gctgggcagg
     1921 ctggggcgga gtcggaagcg gtcactcctg ttaagtccgc attgatgcgg gcgctggaat
     1981 tgtcatcaaa tgatggtagc attgtcttat ccgctggttc gatgttcgtc accgcggaag
     2041 tcatgtctgc atggaaaacg agaaacgaat gaattatcaa agcgactgga acgaaaacga
     2101 agataacttt gacctcacgc tctcacagcg gaccgaggaa ctgtatcgtg tgcccaatat
     2161 ggagcccaac gccgacggtt tgatcgaggc ggtggtgctg ccgttgcgcg acatggtcat
     2221 ttttccgcgc atggtttcgc cgatctttgt ggggcgcgag gcttcgctgt tggcggtgcg
     2281 cgaggcgcaa gccaaagagc agactgtgat cgggttgacc caaaaagacc ctgatttgaa
     2341 tgaccccaac gccgaagact ttttgcccgt cggtgtggaa atggctgtgg ggcggatgct
     2401 gcaaatgccc gatggttcct actctgcgct ggtgcaggga cgcaagcgcg ttgagatcgt
     2461 tgaatttctg cgtgtgaagc cctatatcaa ggtgcgtgcg cgggtgatca atgagccaac
     2521 ttccgccgac aggcagacac aggcgttgat gcgttcggtg ttgagtttgt tcgagcgttg
     2581 tgtgcagttg gatcgctcga tccctgaaga agcgcatctg ttcgcgatga atattgcgga
     2641 gcctggctgg ttgtgcgaca tgatcgccac ttcgctttca ttgacctacg aagcgaaact
     2701 gcggctcctg ctggtgcttg atccgcgtgc gcggcttgga ttactgaaca atttgctggc
     2761 acaggaagtg gatgtgctcg aattggaaga tgagatccat tcgcgtgtgc aaagcgaagt
     2821 ggataaaagc cagcgcgagt tttatctgcg tgagcagatg aagcagatcc aaaacgagtt
     2881 gggcgaaggc gatgtgtttt cgcgtgatgc ttcagatttg aagaaaaagc tggatgccgc
     2941 caacctgcct gatgaagtgc gcaaagtagc catgaaggaa ttggagcggc tcagtcagat
     3001 gccgccgatg gctcccgaag tggggatgat ccgcacgtat attgactgga tcgttgacct
     3061 gccgtggagc aattcctcgc cagataatct tgatgtaaaa aatgccgcga agattttaga
     3121 gcgcgatcac tacggcttga agaaggccaa ggaccgcatt ttggaataca tcgccgtgcg
     3181 ttcgctgaag ccgaaaaagg aacgtcagcc gatcttgtgt tttgttgggc ctcccggtgt
     3241 gggcaagacc tcgcttggcc gttccattgc agatgcgctt ggacgcaagt ttgtgcgtgt
     3301 ttcactcggc ggtgtgcgtg acgaagccga gatccgtggg catcgccgta catacatcgg
     3361 cgcattgccc gggcgtatca tccaaaccat gaaacgggct ggtacgacca atccgctttt
     3421 catgctcgat gaaattgaca agctgtattc agattttcgc ggcgaccccg cctcggccat
     3481 gcttgaggtg ctcgatcccg aacaaaacta cgcttttagt gaccactacc ttgaactccc
     3541 ctttgacctt tcgaaggtca tgttcgtgac cacggccaac tcgctgggca gcattccctc
     3601 gcccctgttg gaccgcatgg agatcatcga attccccggc tacattgaag aggaaaaggt
     3661 tgaaattgcc aaccgttacc tgattccgcg ccaattggaa gaaagcggcg tggataacct
     3721 tggcctggtc tttgaacctg ccgcacttca gcgcatcatc cgtgaataca cctacgaagc
     3781 cggtgtgcgc agtctggagc gcgagatcgg gcgtgtgtgc cgcaaagtag cccgcatgaa
     3841 agcggaaggg aagaaatatc cgcttgcgat cgcgcctgaa cttattgaaa aattacttgg
     3901 cccgcaacag gtctttccac cggaagcgga acgctccgat gaagtgggcg ttgccaccag
     3961 ccttgcatgg acggagaccg gcggcgagat catgtctgtg gaagtggccg tgctcgaggg
     4021 caagggcggg atgcagatga caggtcagat gggggaggtg atgcaggaat ctgcacaggc
     4081 tgcgttgacc tacatcaaat cgcgcgcttc caaattgaag atcgagctgg aagccttcga
     4141 acggctggat attcacgtcc acatgcctga aggcggaatc ccgaaggatg gcccctcggc
     4201 aggtatcaca atggccacag ccatcatctc cgcgctgacg gggcgtccca tcttccatca
     4261 tgtgggcatg accggcgaga tcaccctgcg cggacgcgtg ctcccgattg gcggcgtgcg
     4321 tgaaaaggtt cttgccgcac accgcgccgg gctgaagacc gtgctgttgc cggaaaagaa
     4381 catgaaagat ctggtggacc tgcccaagac cgccaaatcg gaattgaaga tcatcccgat
     4441 caaacacatg gatgatgtgc tggctctggc cctctctgaa aaagtgaccg ttgaacctcc
     4501 gcgcccgaag aagcaagcgc gtgatgacag ccaggaagat tgaaaaagat atacacggat
     4561 gaacgccgat tttttgaatc gcctttgact ccctgtttat ctgtggctaa ataatttctc
     4621 aaagcggcgc ggtgataaaa ttaccgtacc gctttttgtt atcacccctg aaaacggaaa
     4681 aacaaatgtc gaaactgcaa aacaaagttg tcctcattac cggcgcctcc tcggggtttg
     4741 gcgaagatgc cgccatgctc tttgcgcagg agggctgcaa ggttgtgctg gccgcgcgcc
     4801 gcatggaccg tttgcagaat ctcgccgagc ggattcaatc cgcaggtggt gaagcgattg
     4861 caattccagt ggatatcgtc aatgtggccg atgtagataa catggtgcaa accacactcg
     4921 acctttatgg tcatattgac atcctcttca acaatgcggg catcggccgt gtggaatggt
     4981 tcgaaaatca cacccttgaa cgcgacctcg atacattggt gcatgtcaac ctcaccagcc
     5041 ttatgcaagt cacccgttca gtccttcccc atatgctggc gcggggggag gggcacatca
     5101 tcaatatgat ctcagtggcg gggctgcttg ccacaccgct catcaccagt tatgccgcca
     5161 gcaaacacgg tgtgcgcggt ttcaccgatg ccctgcgccg cgaagttgcg ccgctcggca
     5221 tcaaggtcag cggcatttac cccggccccg cctccaccga attcggtcag catgtcggca
     5281 aaaacgaggc ttatcgctct gtccgctcgc ggctcaaaat tcatatgagc tcaagctatg
     5341 tggcccgccg cgtggtggat gtggctaaac gtccgcgccg tagtttgatc attccgtggt
     5401 ggttccgcat cctcaccacg attgaagcgg ttttccccgc ccttgtagat tggatctcat
     5461 actcattcac gaaaaagaaa cataaattgg attaggagac ttaaatgacg accgcccgac
     5521 tcaaaagcct tatcgattcc ctcccaacct cgaacctgga tgctgttatt ctgaatccgg
     5581 gtccaaccct cacacatctg accggtgtcc actttcattt gatggaacgg cctgttgtgc
     5641 tattcgttgc gcccggtcaa gacccggcgc tcgtgctgcc cgaacttgaa ctacccaaat
     5701 tgggattatt cccctacaaa gtgcaggcgt ttgcctacgg tgaaaatccg tcagagtggg
     5761 acgatgcctt tcgaaaggcg gcgcaggccc tgggtctgga tgggaagcgc attggagtcg
     5821 aaccgcgtca attgcgcttg ctcgattttc gtcatgtcaa agcgggggcg cctgaagcgg
     5881 aatacccgga tgcgagcgaa gtactttccc gtttgcgcct gaaaaaagat aaagccgaag
     5941 tggatgcgat gcgtcgtgct gtgaagattg cgcaggatgc cctcgaagcc accatccctt
     6001 tgatcaagat cggaatgacc gaacgtgagt tgtcttccga gttggtcatc caattgttca
     6061 agcaaggctc cgaccctgaa ttgccctttg cgccgatcgt ctctggcgga cccaatgccg
     6121 cgaatccgca cgcttcaccg acggaaagaa aattacaggc aggcgacctg cttgtggtgg
     6181 attggggcgc agccttcgat ggctacatct cagacctgac ccgcaccttc gcagtgggtg
     6241 aggtggatga tgaatgcaac aagatccata aaattgttca agaggccaat gctgcaggtc
     6301 gcgctgctgg cggccctggt gtgtcttgtg ccaatgtgga taaggccacc cgtgatgtca
     6361 ttgaagcatc gggctatgga aaatatttta cccaccgcac cggtcatggc attggcatgg
     6421 aaggccatga agaaccctac atgcgaggcg acaatatgca attgttggaa cccggcatgg
     6481 cctacaccgt tgagcctggc atttatctga cggaccgcaa tggcgtccgt attgaagata
     6541 atgttgtcat cacagaaaac ggcgcagaga gtttaagcga tatgccgcgc gagattcgcg
     6601 tggttggata gacagggttg caagatcctg tgtatgtgat ttgccgtgcc acttccactg
     6661 atgccgaccg cctgacagag atcaccatcg ccgccaaacg gcattggaat tacccggaaa
     6721 agtggatcca attctggctt ccgcaattga ccctgaccgc agagtacatt tcaaaccacg
     6781 aagtttggat ggtcatggca aaggatgccg ctattggtta ttattcgctt gagtattctg
     6841 acggcgagct ttggctggga aatctctggg tattgcctga acacatgggg caggggatcg
     6901 gccgtctgct ttttgaacat gccctgaacc gatgccgcgg gcttggttgc tccaccttga
     6961 agatcgaagc agatcccaat gcccaaagct tttacgaaag aatgggagca tgcaaaatag
     7021 acgagcattg cagcgaagtg gataatcagc cgcgtatatt acctgtcatg gaaattgttg
     7081 taccagcttg atgacactca agatcatgac gcacctcgaa attaagattt cgaggtgcgt
     7141 ttttgtttgc gaccgggatt tgcccttgaa atttcaccaa aacctgctat gattgtttgt
     7201 gattgattga tgagcgatta tgagcgaatt tacccccacc cctgaacggc aaaaacagat
     7261 cctttccctg ctcggcaaac agggacggtt aagcgtcgct gagatcgtgg agcaattctc
     7321 gatcagcgaa gccactgcaa ggcgcgacct ggaaacgctt gcatctcaag gcaaagccca
     7381 acgcgtgcat ggcggcgtga tcgccttgga gcaggcacct ccagagcttc ccattcttga
     7441 acgggaaggc gaacaaacgg acgaaaaagc cagcattggg cgcgccgccg cggaattgat
     7501 cgccgatggc gaaacggttt tcctcggctc agggacaacg gtactcgaag ccgcaaaaca
     7561 tctgcgagat cgaaaaaatc tcacggtcat caccaactcg ttgcctgtgt tgaacgtcct
     7621 tgcgggtatc aaagatatta ccgtcgtttc attgggtgga cagttgcgcg agagcgaact
     7681 ttccttcatc ggtcacatta cggagcaggc actcgccaaa ctgcgcgtgg acaaggtcat
     7741 tatgggcact cgcggaatca gtttggaaca tggtcttacg aatgattatt tgcctgaaac
     7801 tctcacagac cgcgccatcc tgaagatcgg gcggacggtc atcattgccg cagaccacac
     7861 gaaaattaat cgggtggcta ccgctttgct agctccattg aagtccatgc aggtttttgt
     7921 gacggactca catgcagaga agaaattttt gcagtcgttg aaaaagctcg agatcgaaat
     7981 agtcatcgcg tagatcaact tcatccctta ggagaaccat gctcctcaaa aattttcgtc
     8041 cacagtccaa actagtcacc aaaaccacat tggtgaacaa acccaaattc cctgtgatcg
     8101 acgcgcacga tcatctgggt gaacccttcg gtggcggctg ggataaaaag ccgttgagcg
     8161 aattactcga ccttcttgat gaagcaggcg tgactcatta tgttgattta gatggcggct
     8221 ggggcgaaga cattctcgat gcccatctca aacatttcaa agaaggcgca cctgaacgat
     8281 tccaagtctt cggcggcgtg gattggaaca aatggcagga gttggggaat caattcccag
     8341 agtgggcggc gaagcgtttg atcgcacaga aagagcgcgg cgcacaagga ctcaaaattt
     8401 ggaagccgct tggcttgcat gtcaaagacc atcaaggcaa attggt
//
LOCUS       DAOQJC010000154         8333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_4621_length_8333_cov_10.338608, whole genome shotgun sequence.
ACCESSION   DAOQJC010000154 DAOQJC010000000
VERSION     DAOQJC010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4621_length_8333_cov_10.338608"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /gene="asnS"
                     /locus_tag="PLE39_17000"
     CDS             <1..1338
                     /gene="asnS"
                     /locus_tag="PLE39_17000"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNE70084.1"
                     /translation="EHVSVSKIANYVGQDIILKGWVYNRTDKGKLAFLLVRDGSGFVQ
                     CVAFKGDLTPELFDQIAHLPQESSVIITGSVREDKRAPGIPGGFEVGIKDLQVVQAAN
                     LDYPMSLKDHGVDFALDNRHLWIRMQSQWAILRVRVRVMSAVREYLDGQGFFNLDTPI
                     LTPAAAEGTTTLFETEYFDEGSAFLAQTGQLYNEANIFAFGKVYCYGPTFRAEKSKTR
                     RHLTEFWMLEPEIAFCDLDQLMDIEEGLITHIAQSVLKDCSAELKFLGRDLTKLQNIV
                     GPFPRMSYDDAAKYLIDLYEKETDPERKELLKFEWGMDFGAPHETELTKLHDKPVFVY
                     GYPSAVKAFYMEPWPDRPEVCKSVDLLAPEGYGEICGGSERMSDPDKLLARIQKEGLP
                     EEAFKWYLELRKYGSVPHSGFGMGTERVTAWMCGIEHIREAIPFPRTIKRIYP"
     gene            complement(1425..2435)
                     /locus_tag="PLE39_17005"
     CDS             complement(1425..2435)
                     /locus_tag="PLE39_17005"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HNE70085.1"
                     /translation="MATVPGIDVSYWDAGIDWPKVRATGQRFMFAKATEGISYKDLTF
                     DDNWFGAKSAGLLRGAYHFFRCNVDAKKQADYFIDYVRTVKDDGELPPVLDLETNDGM
                     TKDKIVPAVKVWLDRVESAFGKKPIIYSGQYFLQDYVVIAGGGPPTWAKEYPLWLAQY
                     PNQYQEGMKPFLPRGWFSWTFWQYSDKGVVNGINAKVDMNIYNGTLEELYKFAGAKIP
                     DQTPKTHTVKKGDSFESIANSYGVTVRELVMANPQLLVTGSKLTVPVAIAIPQEGDSS
                     STDTGSGDTGTVSTRTYTVKAGDTLSVIAVKYGTTVAMIASANNITNINNIKVGQVLV
                     IP"
     gene            2574..2751
                     /gene="ssrS"
                     /locus_tag="PLE39_17010"
     ncRNA           2574..2751
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLE39_17010"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            3004..3870
                     /locus_tag="PLE39_17015"
     CDS             3004..3870
                     /locus_tag="PLE39_17015"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNE70086.1"
                     /translation="MESTTELSQHYQLIEQAIQYIEINVHRQPELDEIASAIGLSEYH
                     FQRLFTRWAGISPKRFMQFLTKENAKDLLTRSENLLETTHQVGLSSLGRLHDLFVNTE
                     AVTPGEFKSHGAGLIIRYGLHLTPFGKCLIAATERGICHLGFVESSEGNAIDNLVADW
                     NQAKMIEDYKTTAPLIDRIFSTPTPDTPLNLHLRGTNFQIKVWEALLKIPAGALTTYE
                     QIAAQIGNPNALRAVGTAVGHNPIAVLIPCHRVIRKSGEFGNYRYGPARKKALLAKEF
                     AQSRLTSDFQGM"
     gene            3911..4840
                     /locus_tag="PLE39_17020"
     CDS             3911..4840
                     /locus_tag="PLE39_17020"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNE70087.1"
                     /translation="MKSKIWLPLLALYIVWGSTYLGIKVSIETIPPFFHAAVRFLISG
                     MILVVWQRSAGHQMPTRKQWVSAGIIGTFLLLGGNGLVAWAEQFIPSGIAALIIGSMP
                     MFLVIGEAIRPNGVKPTWQGIVGLLVGFAGIFILVGPSEISGSTEKLNPLGVIALLSA
                     CLFWATGSLYSKTADLPKSSLMNTGAQMLVGSVSLLIVSLISGELNGWDVSAVSTRSL
                     VGLAYLIFIGSLVGFVSYGWLLQNAPISLVSTYAYVNPIVAVLLGVWFANEALEPRIW
                     LATAIIIGSVIFINSTKPKVKHEAKAMTAEQGH"
     gene            4902..6242
                     /locus_tag="PLE39_17025"
     CDS             4902..6242
                     /locus_tag="PLE39_17025"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNE70088.1"
                     /translation="MDNKDLLKRVKEDNVKFLSLQFTDVMGAVKSVDMPIRHLEDVLK
                     DGAWFDGSSVEGFARIQESDMRLKIDPETYAVLPWSAADSKRARVFCDIYTPGGDPFD
                     GDPRGALKRILKKIADRGWKLNIGPEPEFFLFKASDGSGVHPVPHDTGGYFDFSSFDE
                     AVVVRTALMEALDAMGLDVEVGHHEVALGQHEIDFRYADALKAADNVLTLKYTVKAIA
                     AQHGLTASFMPKPVYGINGSGMHCHQSLFDIKTGDNLFFDAKDEAHLSPLAYSFIAGQ
                     LEHARALAGVVAPTVNSYKRLVPGYEAPVYIGWAQQNRSALIRIPRYTEGRDKAVRAE
                     LRFPDPSSNPYLAFTVMLAAALDGIDKNMKAPKALNNINLYHLDKAERTKLGVTELPG
                     SLAESLKELEKNEVLKSALGEYLFEAYLRAKWEEWDEFRLRVTDYEITKYLETA"
     gene            6382..>8333
                     /locus_tag="PLE39_17030"
     CDS             6382..>8333
                     /locus_tag="PLE39_17030"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HNE70089.1"
                     /translation="MKIHIKFSLALIVSLLVISNISPSHSITGIARAQANFATVTYDP
                     VNTIITNPERGFLHTNSPCESDIFDLATLQNYRSAEHISLVLCNFYLTSFKSSAISQA
                     TLNTLQAQLNTIRSAGLKVVLRFSYTSTDNVDASLSQILSHIDQLAPILQSNSDVIYV
                     WQAGFIGQWGEWYYTSHFGDQGVISAQQWADRKAVVEKMLQVLPSTRMVSLRAPFYKR
                     TMYQAAAITPDQAFNGSSISRIGQFNDAFVSSPDDMGTYTNIPEEYPWLEAETAYLPM
                     GGENNTWAPPRTNCTTALEEMDRFHWSYINIDYLGTTISNWNSQGCLDTMKKKLGYRF
                     TLIEGTYPDTAQTGSGMDVHISIRNDGFAAPINPRPGELILRNSSSGEIYHLPLKSDP
                     RFWTAGATTTIQETIIVPTNIPAGSYELLLNLPDAEDSLSSRPEYSIQLANANTWESA
                     TGFNNLLHTISISAGIPTTFADVPFDHPLHDYIEALYQAGYTAGCSTDPLMYCPDTIL
                     DRAQSAVFMLRGQMGSTYSPPPAPWDTFADDWTGFEWAEPWAEGMWQEGLTAGCQPSP
                     LMYCPATQLPRVEASVFGLRMKYGVNYTPPAGTGTLFADMTDTSYWGIGWAEQAYQDG
                     LLPACGTDSGTGKPLFCPSELLDRAWG"
BASE COUNT         2071 a         2152 c         2036 g         2074 t
ORIGIN      
        1 gaacacgtca gcgtttcaaa aatagcaaac tatgtaggac aagacatcat cctcaagggc
       61 tgggtctaca accgcacaga caagggcaaa ctggcctttt tgctcgtccg cgatggatcg
      121 ggctttgtgc agtgtgtcgc cttcaaaggt gacttgactc ccgagttgtt cgatcagatc
      181 gcccacttgc cacaggaatc gtctgtcatt attacgggct ccgtccgcga agataaacgc
      241 gcccctggca tccccggtgg atttgaagtt ggcattaagg atctgcaggt cgtgcaagcc
      301 gccaatctcg attaccccat gtcgctcaaa gatcacggcg tggattttgc gctggacaac
      361 cgtcacctgt ggattcggat gcagagtcaa tgggcaattc tgcgtgtgcg cgtgcgggtc
      421 atgtctgctg tacgtgagta tttggatggt cagggcttct tcaaccttga tacacccatc
      481 ctcactcctg ccgcagccga agggaccacc accctgttcg agaccgaata cttcgacgaa
      541 ggttccgcct tcctcgcgca gacggggcaa ctctacaatg aggccaatat ttttgccttc
      601 gggaaagtct attgctacgg tcccaccttc cgcgcggaaa aatccaagac gcgtcgccac
      661 ttaaccgagt tctggatgct cgaacccgag atcgcttttt gcgacctcga tcagttgatg
      721 gacatcgaag agggactcat cacccacatc gcccagtcag tgttgaagga ttgctccgcc
      781 gaattgaagt tcctcgggcg cgatctgacc aagctgcaaa atatcgtcgg cccgttcccg
      841 cgcatgtctt acgatgatgc tgcgaaatac ctgatcgacc tctacgaaaa agagaccgac
      901 cccgaacgca aggagttact caagttcgaa tggggtatgg atttcggcgc cccgcacgag
      961 accgaattga ccaaactcca tgacaagccc gtattcgtct acggctaccc aagtgcggtc
     1021 aaagcctttt acatggaacc ctggcccgat agacccgaag tctgcaaatc tgtggacctg
     1081 cttgctcccg aaggttacgg cgagatctgc ggcggatctg agcgcatgag cgaccccgat
     1141 aagttgctgg cacgcatcca aaaagaaggc ctgcccgaag aagcattcaa atggtatttg
     1201 gagctgcgca agtatggctc cgtcccacac tctggctttg gcatgggcac ggaacgtgtc
     1261 accgcatgga tgtgcggcat cgaacacatc cgcgaggcaa ttcccttccc gcgcacgatc
     1321 aagagaattt acccgtaaga aagcagaaac agatcgttgg tttcaaatgc aaaagagctc
     1381 tggaaaattc ttccagagct ctttttatat attacctttt cgttttacgg aatgaccaac
     1441 acctgtccca ctttgatgtt gttgatattt gtgatgttgt ttgcagaggc gatcattgcc
     1501 acagtagttc catatttaac ggcaatgacg gagagcgtgt cacctgcctt cactgtgtag
     1561 gtacgggttg atactgtccc agtatcgccg gaccctgtgt cggttgaaga tgaatcgcct
     1621 tcttgtggaa tggcgattgc cacagggacg gtcaatttcg atccagtaac aagaagttgc
     1681 gggttggcca tcaccagttc acggacggta acaccatagc tattggcaat ggattcaaaa
     1741 ctgtctccct ttttgaccgt gtgggtcttt ggagtttggt cgggaatttt tgcgcctgca
     1801 aatttataca attcctcaag tgtgccattg tagatgttca tatccacttt ggcattgatg
     1861 ccattcacca cgcccttatc gctgtattgc cagaaggtcc aactgaacca gccgcgcggc
     1921 aggaagggtt tcatgccttc ctgatattga ttgggatatt gtgccaacca gagcggatat
     1981 tccttcgccc aggtgggagg gccgccgccc gctataacga cataatcctg caaaaagtat
     2041 tggcctgaat aaatgatggg ctttttgcca aaagcagact caacacggtc aagccagacc
     2101 ttgaccgcgg gcacgatctt atccttggtc atgccatcgt tggtctcgag atccagcacg
     2161 ggcgggagtt cgccgtcgtc ttttactgta cggacataat cgataaaata atccgcctgt
     2221 ttcttcgcat ctacgttaca tcgaaaaaaa tgatacgcgc cgcgcaaaag ccccgccgat
     2281 ttcgcgccga accaattgtc atcaaaggtg aggtctttgt agctgatacc ttcggtggcc
     2341 ttggcaaaca taaaccgctg gcctgtggcc cgcacttttg gccagtcgat ccctgcatcc
     2401 caataggaaa catcaatacc tggaactgtt gccatgcttt cctccgtcat cgataaatta
     2461 ttcggataca acatacagag ggtagcacaa tttttatttt aaatcaatca atttccaggc
     2521 gcgggggttt aatgattatc tacccttgta ccattgatta tggtataaga actgcacccc
     2581 tgctgtgttc gtgatattgc ctccacgttt tgagccaata cccactaaaa gggtccttaa
     2641 cttaattaga aataacgcga taggcctgag ccaaggcggc gtttctaggt agggacccac
     2701 ctgcaaatgc aggttcaaaa cctggtgata cacggcataa gcggggtaat tttttgcatg
     2761 aaaatacatg aaaccagatt agcggacgtg gcggatataa gtcgttgatc acttgtattt
     2821 gttgagagga cgtttctttt ttaataccca tgcgtattga aacaggtgtg cccgggcatg
     2881 tctgtttttt attttacaga tcaacctgat ttgtcttttg aaagcaagga gaaaatgagc
     2941 aagaatcggg tggtttccgt gagtaaagac caatacaatc tcctgcatca aggagattaa
     3001 tttatggaat ccacaactga actttcacag cattatcagc ttattgaaca ggccattcaa
     3061 tacatcgaaa tcaatgttca ccgccaaccc gagttggatg agatcgcctc tgccatcggc
     3121 ttgagcgagt atcatttcca gcgtttattc acccgctggg cgggcatcag ccccaaacgt
     3181 ttcatgcaat tcctcaccaa ggaaaacgcc aaagacctgc tcacccgatc agagaattta
     3241 ctggagacaa ctcatcaggt gggcctctcc agcctgggac gcctgcacga tctcttcgtc
     3301 aacaccgaag cagtgactcc cggcgagttc aaatcgcatg gagcgggcct catcatccgt
     3361 tatggcttgc atctcacacc gtttggaaaa tgcctgatcg cagccaccga gcgcggaatt
     3421 tgtcatctgg gatttgtgga atcgagtgaa ggcaatgcca tcgacaacct ggtagcagat
     3481 tggaatcagg cgaagatgat cgaagactac aaaaccaccg ccccactgat tgaccgcatc
     3541 ttttccacgc cgacacctga tacgcctctc aatcttcatc tacgcgggac gaatttccag
     3601 atcaaggttt gggaggcatt gctcaaaatt cccgctgggg cattgaccac ctacgaacag
     3661 atcgccgcac aaatcggcaa ccccaatgcc ctgcgtgccg tgggaacagc cgttggacac
     3721 aacccgatcg cggtcttgat cccatgtcat cgggtgattc gcaagagcgg cgagtttgga
     3781 aattatcgct acggccctgc gaggaagaag gctttgcttg caaaagaatt cgcccaaagc
     3841 agactaacaa gtgactttca aggaatgtag tcgaaggact tcattccttc aggatgaaaa
     3901 aaaggaaaaa atgaaatcga aaatctggct gccattgttg gcactttaca tcgtttgggg
     3961 ctcaacgtat cttggaatta aagtatccat tgagaccatc cctccgtttt tccatgcggc
     4021 ggtacgtttt cttatttctg ggatgattct ggtcgtctgg cagcgcagcg caggccacca
     4081 aatgcctacg cgcaagcaat gggtttctgc tggtatcatt ggaacattcc tcttgctggg
     4141 cgggaatggt ctggttgcct gggctgagca attcattcca tcgggcattg cggcgctcat
     4201 cattggctcc atgcccatgt ttctcgtaat tggtgaagcc attcgcccca acggagtcaa
     4261 acccacctgg cagggaatcg ttggtctgtt ggtcggtttt gcgggtatat ttattcttgt
     4321 tggtccttca gaaatttcag gaagcaccga aaagttgaat ccgttgggtg tgatcgccct
     4381 gctgtcggct tgtctatttt gggccacggg ctctctgtac agcaaaacag cagacctgcc
     4441 aaaatcttcg ttgatgaata caggcgcgca aatgctggtc gggagtgtca gcctgttgat
     4501 cgtttcgctg atttcgggcg agttgaatgg ctgggatgtt tcagccgtat ccacccgctc
     4561 gctggttgga ttggcatatc ttatctttat aggttcactg gtcgggtttg tttcatatgg
     4621 ctggctgctt caaaatgcac ccatctcgct tgtttcgacg tatgcttatg tcaacccgat
     4681 cgttgcggtt ttacttgggg tttggtttgc caatgaagca cttgagccgc gtatctggct
     4741 ggcaacagcc atcatcatcg ggtcagtgat ttttattaac agtaccaagc caaaggtcaa
     4801 gcacgaagcg aaagcgatga cggcagaaca aggtcattaa tcagaaaaca gtgatcagta
     4861 gaaaatgatc agtggtcaaa catcaaaaaa ggagaaacaa aatggataac aaagatctgc
     4921 tcaaacgtgt aaaagaagac aatgtgaagt tcctttcgct ccagttcaca gatgtgatgg
     4981 gtgcggtcaa gagcgtggat atgccgatcc gccacctcga ggatgtgttg aaagacggcg
     5041 catggttcga tggatcatcg gtggaaggtt ttgcgcgcat tcaggaaagt gacatgcgcc
     5101 tgaagattga ccccgagacc tatgcagtgc tgccctggtc ggctgcagat tcaaaacgcg
     5161 cgcgtgtgtt ctgtgatatt tacaccccag gcggcgatcc cttcgatggc gatccgcgcg
     5221 gcgcgttgaa gcgcattctc aagaagatcg ccgaccgcgg ttggaaactc aacattggcc
     5281 ctgagcctga gttcttcctg ttcaaggctt ctgatggaag cggcgttcat cccgtccccc
     5341 acgatacggg tgggtatttc gatttctcat ccttcgatga agccgtggtt gttcgcaccg
     5401 cactgatgga agccctcgat gccatgggac tcgatgtgga agtgggtcac cacgaagttg
     5461 cattgggcca gcatgaaatt gacttccgct atgcagatgc gctcaaggcc gccgacaacg
     5521 tgctgacctt gaagtacacc gtgaaagcga ttgccgccca acacggattg accgcatctt
     5581 tcatgccgaa gcctgtgtac ggcattaatg gctctgggat gcactgccac caatctctct
     5641 tcgatatcaa gacaggcgat aacctgttct tcgatgcgaa ggatgaggcg cacctttccc
     5701 cgttggctta ttccttcatc gcgggtcagt tggaacatgc ccgcgcgctg gctggcgtag
     5761 tagccccgac cgtgaattcc tacaagcgtt tggttcctgg ttatgaagcc cctgtgtaca
     5821 tcggctgggc tcagcaaaat cgctcagcgc tcatccgcat tccgcgctac accgaaggcc
     5881 gtgacaaggc tgtccgcgct gagttgcgct tccccgaccc atcctccaac ccgtatctgg
     5941 ctttcactgt gatgctggcc gccgctctcg atggcattga caagaacatg aaggcgccga
     6001 aggcgttgaa caacatcaac ctgtatcacc tcgataaggc cgaacgcacc aagctgggtg
     6061 tgaccgaatt gcctggttca ctggccgaat ccttgaaaga actggaaaag aacgaagtgt
     6121 tgaagtcagc gctcggcgaa tacctgttcg aggcctacct ccgcgcgaaa tgggaagagt
     6181 gggatgagtt ccgcctgcgc gtgacagatt acgagatcac gaagtatctg gaaaccgcgt
     6241 agtaaacgta gtacaaatta aacaaaggct cggagggatt ttggtatcct tccgagcctt
     6301 tgttttccta tataatgaat taacaaaata tgtgtcgtgg cttattctca atcttattgg
     6361 tactcacatg gagagagtct tatgaaaatt cacataaaat tcagtctggc ccttatagtt
     6421 tcactgttgg taatttcaaa catcagtccc agccattcga ttacaggaat tgcacgagca
     6481 caggcaaatt ttgcaacagt aacgtacgac cctgtaaaca ccataatcac caatccagaa
     6541 agaggttttc tacataccaa ttccccctgt gagagtgaca ttttcgacct tgccactctt
     6601 caaaattacc gctctgctga gcatattagc ctggtacttt gcaacttcta cctcaccagc
     6661 tttaaatctt ccgcgatcag ccaggcaacc ttaaacaccc tgcaggcaca attgaacaca
     6721 attcgctccg caggcttgaa ggttgtgctt cgtttttcat ataccagcac ggataatgta
     6781 gatgcaagcc tttctcaaat cctgtcgcac atagaccaac tcgcgccgat ccttcagtcg
     6841 aatagtgacg tgatctatgt ctggcaggcg ggttttatcg gtcaatgggg ggaatggtat
     6901 tacaccagcc actttggcga ccagggtgtt atttcagccc aacaatgggc agatcgaaaa
     6961 gccgtcgtcg aaaaaatgct gcaagtcctg ccatcaacgc ggatggtttc attgcgcgca
     7021 ccattctata aacgcaccat gtatcaagca gcagccatca ccccagatca ggcattcaat
     7081 ggcagttcca tatcacggat agggcaattt aatgacgctt ttgtctccag ccctgacgat
     7141 atgggaacct ataccaatat acctgaggaa tatccctggc tggaagctga aaccgcctac
     7201 ctcccaatgg gaggtgaaaa caatacatgg gcacctccac gcacaaattg cacaacagca
     7261 ttggaagaga tggatcgttt tcattggtca tatatcaata tagactatct gggcacaacc
     7321 atctccaatt ggaatagtca ggggtgttta gacaccatga agaaaaaact gggatatcgc
     7381 ttcacgctta tcgaaggcac atatcccgat acagcacaga caggcagtgg aatggatgtt
     7441 catatttcca ttagaaatga tggattcgcc gcaccaatca atccacgacc tggcgaattg
     7501 atcttgcgaa actcctcttc tggcgaaatt tatcatcttc ccctgaaaag cgaccctcgt
     7561 ttttggacgg ctggagcaac cactacaatt caagaaacca tcatagtacc aaccaacatc
     7621 cccgctggca gttatgaact tttgctaaac ctccctgacg cggaagattc cctgagttca
     7681 aggccagagt attcgataca gttggcaaac gcaaacacat gggagtctgc tacaggattc
     7741 aataatctgc tccacacaat atcaatttct gctggaatcc ccaccacctt tgcagatgta
     7801 cccttcgacc atcctctcca tgactatatc gaagccttat atcaggctgg atacacggca
     7861 ggatgcagca ctgacccttt aatgtattgt cccgatacca tcctcgaccg cgctcaatcg
     7921 gctgtcttca tgctgcgcgg acaaatgggt tccacctatt cccctccacc cgccccgtgg
     7981 gataccttcg cagatgactg gactggtttt gaatgggctg agccctgggc tgaaggcatg
     8041 tggcaggagg gtctcactgc tggatgccaa cccagcccgt tgatgtattg tcctgccaca
     8101 caacttccgc gcgttgaggc tagtgtcttt ggtttacgca tgaagtatgg tgtaaattac
     8161 actcccccag cagggactgg taccttattt gcagatatga ccgatacaag ctattggggt
     8221 atcggttggg cagagcaggc gtatcaggat ggattactcc ccgcctgtgg gacagacagt
     8281 ggaacaggca aaccgttgtt ctgcccctct gaattgcttg atcgagcctg ggg
//
LOCUS       DAOQJC010000155         8299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_4654_length_8299_cov_8.105774, whole genome shotgun sequence.
ACCESSION   DAOQJC010000155 DAOQJC010000000
VERSION     DAOQJC010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4654_length_8299_cov_8.105774"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2461)
                     /locus_tag="PLE39_17035"
     CDS             complement(<1..>2461)
                     /locus_tag="PLE39_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70090.1"
                     /translation="TLTIKYPGMTGIHNYVKMPDAISNSATGGSVEERTWQNNESVMT
                     VLRGFYDVLIVKGSASFIYDDVDCTALTCTLDKASLTVKFPGITSVHTYVRKSDNVAG
                     TATGAQVASQTYKADQAVFADLPNGFYDVIVVKGAQTKVIDNVTVLGGWATVDKIVAT
                     LTVEFPGIAGVHTYAKVNDGVVGTAAGGSVEDRTYKNDEASMVVLKSTYDVIVVKGAK
                     QKVIDAVDCTGETCVVENIVATLTVKFPGISGVHTYVKVNDGVEGVSGGDVENRTYKN
                     DEASMVVLKNTYDVVVVKGAKQKVIDAVDCTGETCVVENIVATLTVKFPGIAGVHTYV
                     KVNDGVAGASGGDVENRTYKNDEASMVVLKNTYDVVVVKGAKQKVIDAVDCTGETCVV
                     ENIVATLTVKFPGISGVHTYVKVDNGVAGTATGGDVENRTYKNNETSMVVLKNTYDVI
                     VVKGAKQKVIDAVDCTGNTCVIEDIVATFTLKFPGKTGVHVYVKVIDNVAGTAAGGDV
                     ENRTYQNNSTTMVLLKNTYDVVVKIGADSYILDDVNCTGNTCTYSLAVVKLVNSTNAA
                     LAGGVVKYYDGSWHPVGNTNASGLMAFGYLGTSTNLLFSVDYAFTHNEKWQNIGTNST
                     VLFQTKKVEVQLKNSVGTLVDAGVVKYYTGAWHDFGNTSGGKVSMELLPSNVLFSMDN
                     QFVRNEKWQNTTTDATVVFQMKNVEVQLKDSSGTLMDTGTVKYYTGAWHELGSTSGGK
                     VNVDLLPANILFSMDNQFVRNEKWQNTGSNATVLFQTKKVVVQLKDSTGALMDTGTVK
                     YYTGSWHTFGTTSGGEVSMELL"
     assembly_gap    2462..2561
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2562..5257)
                     /locus_tag="PLE39_17040"
     CDS             complement(<2562..5257)
                     /locus_tag="PLE39_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70091.1"
                     /translation="MKHIKSFNVMVAVLLVCTALSFQNVTATGVSVFNSIPDPLPGNL
                     PSQPYQAQQAAEFGDHIQLAGTSRNLSSVTVTMSSWARHSEYPSLSEVGFAHPLTLNI
                     YNVDKSGTVPALGALIASVTQEFVMQWRPEGDPTCPDTGYGAGFAWRAVNGTCYNGFA
                     FNIVFDLSEEGIVLPDEIIYGIASNTETWGYDPIGTPGPYNSLNFAVNNISAPSTGID
                     VEPDAVFFNTETAAWYADGGAGGVGIFRRDTGWTGYVPAVRFDVEEAVVTPELTVKVQ
                     DSLGNPVEGAVITYAVGSPNAGWQTFGTTAADGTISRSDLTVGTNYHFYASYNASTST
                     SQALVFDGDDEVVFETVLVKAKVETCLGAAIEGAAVTYGSVNGGFLSFGTTGSDGFVS
                     KELFPGYDRTFYASVNHTSSALQTATVVEQGDPLVTFETTKVTINYSGSVNHGSANGG
                     WYPFTKPAMEMFAGARTFLINGTQVAATVPECDFSTGDLTVKFPGIGSVHTYLKKSDG
                     VNGVANGVQVASLTWKNDEAVFADVPNDVYDIVVVKGAQTKIVDNVIVLGDKTVEGLV
                     STLTVEFPGVGSVHAYAKMPDGIAGVAGGGGVEERTWKNDLAELIVLKGTYDVLVVKG
                     AKNYVVDNVDCTGETCLVNGIVATLTVKFPGIGSVHTYARMPDGGGAVEERTWKNDQS
                     EMVVLKGVYDVSVVKGAKTYIVEDVDCTGNTCLVDGIVATLTVKFPGIGSVHTYAKMP
                     DGAAGVAGGGGVEERTWKNDQSEMVVLKGTYDVQVVKGAKTYVVDNVDCTGTSCQVDG
                     IVATLTVKFPGIGSVHTYAKMPDGVANTAGGGGVEERTWKNDQSEMVVLKGMYDVSVV
                     KGAKTYVADDVDCSGDTCLVEGIVATLTIKYPGMTGIHNYVK"
     regulatory      complement(5371..5481)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(5726..6430)
                     /gene="cysE"
                     /locus_tag="PLE39_17045"
     CDS             complement(5726..6430)
                     /gene="cysE"
                     /locus_tag="PLE39_17045"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017638459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNE70092.1"
                     /translation="MLKTIREDVQSVLDRDPAARSVWEILLCYPGVYAIWIHRVAHRL
                     WKNGFKLIARWLSQLSRSLTGIEIHPGATIGRNFFIDHGMGVVIGETAEIGENVTLYH
                     GVTLGGTSLQKTKRHPTIEDHVVIGAGAKVLGAITIGAHSRIGANAVVVKSSPPNSVI
                     IGVPGQIIVRSQDKAINNKPDLEHGLLPDTIGETLAALVSHVERLEKRLDGHTSVSPA
                     LHTPDHGIWHGEDFAI"
     gene            complement(6498..7436)
                     /gene="cysK"
                     /locus_tag="PLE39_17050"
     CDS             complement(6498..7436)
                     /gene="cysK"
                     /locus_tag="PLE39_17050"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNE70093.1"
                     /translation="MKIANNVTDLIGNTPLVRLNRISDGTKAEVVAKLEFYNPAHSVK
                     DRIGAAMIDAAERDGLLTKDTTIVEPTSGNTGIALAMVAAARGYKIILTMPETMSKER
                     RMLLRAYGAELVLTPGPDGMGGAIRKAEEIAGSDPKKYFLPQQFKNLANPEIHRKTTA
                     EEIWRDTDGKIDFLVSGIGTGGTITGTGEVLKSRKPSLKAIAVEPDASPVLSGGAKGP
                     HPIQGIGAGFVPDILNTKIYDEIIRVKNDDAFQTARRMAREEGLLVGISSGAATWAAL
                     QVANRAENAGKLIVVIIPSFGERYLSTALFADLADG"
     gene            complement(7822..>8299)
                     /locus_tag="PLE39_17055"
     CDS             complement(7822..>8299)
                     /locus_tag="PLE39_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70094.1"
                     /translation="GSHRGYAPEAELGLELAGLADIPLMSFGKLEFIAAQHGLNPATL
                     MKPSPFHALAGIIAAWTGNELSALNAVYEWHTSGKLNGKFNELPKSFELYVIEDTMGG
                     VRATRAAGEVLREAGFGVSVHALGLTSGIEQKAEAFRKADVPFFENWEDLIAFGNL"
BASE COUNT         2043 a         2296 c         1785 g         2075 t
ORIGIN      
        1 agcaattcca tgctgacttc accgccagag gttgtgccga aggtatgcca tgaacctgtg
       61 tagtatttga ccgtgccagt atccatcagg gcaccagtgc tatccttcaa ttgcaccacg
      121 actttcttgg tctggaataa tacggtcgcg ttgctgcctg tattctgcca tttttcgtta
      181 cggacgaatt gattatccat gctgaacagg atgttcgcag gcagtaaatc cacattcact
      241 ttgccgccag aggtgctgcc aagctcatgc catgcacctg tgtaatattt gaccgtgccg
      301 gtgtccatca gcgtgccgct gctgtccttc aactggactt caacgttctt catctggaag
      361 acaaccgtgg catctgtagt ggtattctgc catttttcat tgcgcacgaa ctgattgtcc
      421 atgctgaaca ggacgttgga aggcagcaat tccatactga ccttgccgcc ggaggtattg
      481 ccaaagtcgt gccaggcacc tgtgtaatat ttgaccacgc cagcatcgac gagggttcca
      541 acgctgttct tcagctgtac ttcaaccttt ttagtctgga acagaacggt cgaattggtg
      601 ccgatattct gccacttttc gttgtgcgta aacgcataat ccacgctgaa cagcaggttg
      661 gttgatgtcc caaggtaccc aaaagccatc agtccgcttg catttgtatt gccaacggga
      721 tgccaactgc catcataata tttcactaca cctccggcca gcgccgcgtt cgtgctattg
      781 accagtttga ccacagcgag gctgtaggtg caagtattcc cggtgcagtt gacatcatcg
      841 aggatatagg agtctgcgcc gatcttgacc accacgtcgt atgtgttctt gagcagtacc
      901 atcgtagtgg aattgttctg ataggtgcga ttctctacat cgccgcctgc ggctgtgcca
      961 gccacattgt cgatcacttt gacatagaca tgtacaccgg tcttaccggg gaatttgagc
     1021 gtaaaggttg caacaatgtc ttcgatcaca caggtattcc ccgtgcaatc caccgcatca
     1081 atgaccttct gctttgcacc cttgaccacg atcacatcat aggtgttctt caaaaccacc
     1141 atggaggttt cgttgttctt gtaagtacgg ttctccacat cgccgccagt ggctgtgcca
     1201 gcaacgccgt tatccacctt cacataggtg tgaacgccgc tgataccggg gaatttcact
     1261 gtcagtgtag cgacgatatt ctccactaca caggtttcgc ccgtgcagtc caccgcatca
     1321 atgacctttt gcttcgcacc cttgaccaca accacatcat aggtattctt cagcaccacc
     1381 atggatgctt cgtcattctt gtaggtgcgg ttttccacat cgccaccaga ggcgcccgcc
     1441 acaccgtcat tcactttgac gtaggtatgc acaccggcaa taccggggaa tttcaccgtc
     1501 agtgtagcaa cgatattttc caccacgcag gtttcgcccg tgcagtccac cgcgtcgatg
     1561 accttttgtt tcgcgccctt gaccacaacc acatcgtagg tgttcttcaa aaccaccatg
     1621 gatgcttcat cgttcttgta ggtgcggttt tccacatccc cacccgacac gccttccacg
     1681 ccatcattca ctttgacgta ggtatggacg ccgctgatac cggggaattt caccgtcagt
     1741 gtagcgacga tattttcaac cacgcaggtt tcgcccgtgc agtccaccgc gtcgatgacc
     1801 ttttgtttcg cgcccttgac cacaatgacg tcataggtgc tcttcaatac caccatggat
     1861 gcttcgtcat tcttgtaggt gcggtcttca acacttccgc ctgcggctgt gccaaccaca
     1921 ccatcattca ccttggcata ggtatggaca cccgcaatac cggggaattc aacggtcaac
     1981 gtggccacga tcttatccac ggtggcccaa ccgccaagca cggtcacgtt atcaatgacc
     2041 ttggtctgtg cgcccttcac cacgatcaca tcataaaaac cgttcggcag atcggcaaag
     2101 acagcctgat cggctttata ggtttgagat gcaacctgtg ctccggtcgc cgttcccgca
     2161 acattatcgc tcttacgcac ataagtatgt acgctggtaa ttccggggaa cttcaccgtg
     2221 aggctggctt tatccaatgt gcaggtcagg gccgtgcaat ccacatcgtc ataaatgaac
     2281 gaggcgctgc ccttcacgat cagcacatca tagaaaccac gcaaaacggt catgacagat
     2341 tcattgttct gccaggtgcg ttcttccaca gatccgcccg ttgccgaatt gctgatcgca
     2401 tcgggcatct tgacgtagtt atggattccc gtcatgccgg ggtatttgat ggtcaatgtg
     2461 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natcttgacg tagttatgga
     2581 ttcccgtcat gccggggtat ttgatggtca atgtggcaac gattccctcc acaagacagg
     2641 tatcgccgct acagtcaaca tcatctgcta cataggtctt ggcacccttg accacggaaa
     2701 catcatacat gcccttgaga acaaccattt cagactggtc attcttccag gtgcgctctt
     2761 ccacaccgcc gccgccagca gtattagcga cgccgtcggg catctttgca taagtgtgga
     2821 cactgccaat accagggaat ttcacggtca atgtggcgac gatcccatcc acctgacaac
     2881 tggtgccggt acaatcgaca ttatccacca cgtaggtctt cgcgccttta actacctgta
     2941 cgtcgtaggt gcccttcagc acgaccatct ctgactggtc attcttccag gtgcgctctt
     3001 ccacaccgcc accaccagcc acgcccgcag cgccatcagg catctttgca taggtatgga
     3061 cactgccaat gccggggaat ttcacggtca atgtggcgac aatcccatcc accaggcagg
     3121 tattgccggt acaatccaca tcttcgacaa tataggtctt tgctcccttt actacagaaa
     3181 catcatacac acccttgagg acaaccattt cagattggtc gttcttccaa gtgcgttctt
     3241 ccacggctcc accgccatcg ggcattctgg cataggtatg gacactgcca atgccgggga
     3301 atttcaccgt cagcgtggca acaattccat tcacaaggca ggtttcgccg gtgcaatcta
     3361 cattgtccac tacatagttt tttgcacctt tgaccaccaa cacatcatag gtgcctttga
     3421 gaacgatcag ttccgccagg tcgttcttcc aggtgcgctc ttccacaccg ccgcctccag
     3481 ccacacccgc aataccatca ggcatctttg cataggcatg aacactgcca acgccgggga
     3541 attcaacggt caaagtggag accaaacctt ccacagtttt atcaccgagc acaataacgt
     3601 tatcaacgat tttggtctgt gcgcccttta ccaccacaat gtcatacaca tcgttgggca
     3661 catcggcgaa gaccgcttcg tcattcttcc atgtgaggga agcaacctgc acaccattcg
     3721 caacaccatt gaccccatca ctcttcttca agtaagtgtg aacgctgcca atccctggga
     3781 atttaacggt gagatcaccc gtggagaaat cacattcagg tacagttgca gcaacctgcg
     3841 taccgttgat aaggaaggtg cgcgcaccgg caaacatttc catggctggc ttggtaaatg
     3901 gataccagcc gccgttggcg ctgccatgat tgaccgaacc gctgtagtta atcgtcacct
     3961 ttgtggtttc gaatgtaacc aacggatcac cttgttcaac aactgttgcg gtctgcaatg
     4021 ccgaagaagt gtgattcacc gatgcataga acgtgcggtc atagccaggg aacagttcct
     4081 tcgaaacaaa accatcacta ccggttgtac caaaggatag gaaaccgcca ttcacactac
     4141 cataagtgac cgccgcgcct tcaattgcgg cacccaggca ggtttccact tttgccttga
     4201 ccagaacggt ttcaaagaca acctcatcat cgccatcgaa aaccaacgct tgcgaagtgg
     4261 atgttgaggc gttgtaactg gcatagaagt ggtagttcgt gccaacggtc aggtctgaac
     4321 ggctgatcgt gccatccgca gcggttgtac caaatgtctg ccaaccggca ttagggctgc
     4381 caaccgcgta ggtaataaca gcaccttcta ctgggttacc cagactgtcc tgcactttga
     4441 ccgtcaattc aggcgtaaca actgcttcct caacatcaaa gcgaactgca ggaacatacc
     4501 cggtccaacc agtgtcacgg cggaaaatac caactccacc cgcaccaccg tctgcatacc
     4561 atgccgctgt ttcggtattg aagaaaactg cgtctggttc cacatcgata cctgtacttg
     4621 gcgctgaaat attatttacc gcaaaattca gcgagttgta agggccagga gtaccaatcg
     4681 ggtcatatcc ccaagtttcc gtgttcgatg cgataccgta aatgatctca tcaggaagaa
     4741 caataccttc ttcgctcaaa tcaaatacga tattaaaggc gaagccattg tagcaagttc
     4801 cattgaccgc tctccaggca aagcctgccc catatccagt gtctggacag gtggggtcac
     4861 cctcaggccg ccattgcata acaaattcct gagtcacact tgcgatcaac gcaccaagcg
     4921 ctggaactgt tccgctctta tctacattgt aaatgttaag tgtaagtgga tgagcaaatc
     4981 ccacctcaga caggcttgga tactccgaat gccgtgccca actgctcatg gtcaccgtca
     5041 cactggaaag atttcgagat gtcccagcca attgaatatg atccccaaac tctgccgctt
     5101 gttgggcctg atatggctgg ctgggtaaat tcccaggcaa tggatcggga atgctgttaa
     5161 aaacagatac gcctgtggcg gttacattct gaaacgatag agcggtgcat acaagcaaca
     5221 ccgcaaccat cacgttaaaa gacttgatat gtttcatttt ttctcccttt caaaaacatg
     5281 gactgcacga acaaaaatga agtgccattt atgtcatcct gcgttccttt ataaaacacc
     5341 tcctttttcc ggtttaaatt aaaaaccggc catatgggcc ggtttcgcta ttagctcccc
     5401 aaagacctat ctgttggcat ggtaagtgcc acaagataag cgaccaaata aagtcattgg
     5461 atctttgctt ccagaaaaat attcaatttt catctcatgt ctaaaggaaa ttactgcttc
     5521 atcacaaaac aaatataaga cttccatccc tgtcataact taccaatagg tttgtttctc
     5581 aatgggttac ttctaaaaat cccacagaag atgttgcagt agaatcagta tacaagtttt
     5641 atttaacaat tacaagtgtt tcttattatc tctttcctaa ttggacaaat tgggcttact
     5701 tcatccagcg acacatcttt atggattaaa ttgcaaagtc ttcaccatgc caaataccat
     5761 gatcgggggt atgtaaggca ggtgaaacgg atgtatgacc gtccaatctt ttttcgaggc
     5821 gttccacatg agaaacaagg gcagctaacg tctcaccgat cgtatcgggc aagaggccat
     5881 gctctaggtc tggcttgtta ttgattgcct tatcctgact acgaacaatg atctgccccg
     5941 gcacaccgat gatgactgag ttcggcggag atgatttcac tacgactgca tttgcgccga
     6001 tacggctatg cgcgccaatt gtaatagccc ccaatacctt cgcgcccgca ccaatcacaa
     6061 catgatcttc gatggttgga tgcctcttgg tcttttgcaa gctggtgccg cccaatgtaa
     6121 ccccatgata caaggtgaca ttctcaccga tctccgcagt ctcaccaatg acaaccccca
     6181 tcccgtggtc aatgaaaaag ttgcggccaa tggtggcccc gggatggatc tctattcccg
     6241 tcaaactgcg cgagagttgt gaaagccagc gtgcgatcaa tttaaatcca tttttccata
     6301 gccggtgagc cacgcggtga atccaaatgg catatacacc ggggtaacac aaaaggatct
     6361 cccacacgct acgagcggca ggatcacgat ccaatacaga ttgaacatct tctcgaatgg
     6421 tcttcaacat atttactcac tccatgcaga gatgcggcag gcctgccgca tctctgcatg
     6481 tgaattaaaa aaaacagtta tccgtcagcc aggtcagcaa acaaagccgt ggacaaataa
     6541 cgttcaccaa atgaagggat gatcaccacg atcaacttgc cagcattttc agcacgattt
     6601 gccacctgca aggcagccca agtggcggca ccggaagaaa taccaaccaa caatccttct
     6661 tcacgcgcca tgcggcgggc ggtctggaag gcatcatcat tttttacacg gatgatctca
     6721 tcatagatct tcgtgttgag gatatctgga acaaagcccg caccaattcc ctgaatggga
     6781 tgtggtcctt ttgcaccacc agacaaaaca ggagaagcat cgggttctac agcaattgct
     6841 ttcaaagatg gtttgcgcga tttaagaacc tcacctgtac cggtgatcgt tccaccagtg
     6901 ccaatccctg aaaccagaaa atctatcttc ccatcggtat cgcgccaaat ctcttcggct
     6961 gtcgttttac gatggatttc aggattcgcc aggtttttga actgttgcgg aagaaaatat
     7021 tttttcgggt cggaacctgc gatttcttcg gctttgcgaa tcgcgccccc cattccatct
     7081 ggaccaggtg tcaaaaccaa ttctgcgcca taagctcgca acaacatgcg gcgttccttg
     7141 ctcatcgttt caggcatggt caggatgatt ttatatccac gtgcagcagc aaccatagcc
     7201 agagcgatac cggtattgcc gctggtaggc tccacaatgg tcgtatcttt tgtgagcaaa
     7261 ccatctcgtt ccgccgcatc gatcatggca gcaccaattc ggtcttttac agaatgagcc
     7321 gggttgtaaa attccagttt ggcaaccacc tcggcttttg ttccatcaga aatacgatta
     7381 agacgaacca aaggtgtatt tccgatcagg tctgtaacat tgtttgcgat tttcatattg
     7441 ctctccttat aatgatattg gttttaaata aataaaacgg ctgtctgcca accggaataa
     7501 atcccggtct acgcaaacga tgggcgcaat tcacaagccg tgccagcggc ttggagaagt
     7561 tgcgggatca catccaccgt ttacggcagt cagccgtttg tatctctgta aagaggcggt
     7621 ggttagcgac cccgccaggt acacatgcag ttcaaaaatt ctattggttt catcattgcc
     7681 tttagaaaag gcgattcgcc tttttcttac ctcgtaaatt tgtcatacag atattttgag
     7741 ccgcttcaaa gccttgaagg ttacgcatta taaccataca agaacttttt tcgaaggtta
     7801 atcacaaatc tttcgacggc tctataaatt accgaacgcg atcaaatctt cccaattttc
     7861 aaaaaatggc acatccgctt tgcggaatgc ctctgccttc tgctcgatgc ctgaagtcaa
     7921 gcccagcgcg tgtacactca ccccaaaacc tgcctctcgc aagacctccc ccgccgcccg
     7981 tgtcgcacgc acaccgccca tcgtatcttc gataacgtac aactcaaacg actttggcag
     8041 ttcgttaaat ttcccattca acttacccga cgtatgccat tcatacaccg cattcaatgc
     8101 cgacaattca ttccccgtcc atgccgcaat gattccagcc agcgcgtgga acggcgatgg
     8161 cttcatcaac gtcgctgggt tcaagccgtg ttgcgccgcg ataaattcca actttccaaa
     8221 ggacatcaac ggaatatccg ccagccctgc caactccaag cccaactccg cttcaggcgc
     8281 gtacccacga tgtgaacca
//
LOCUS       DAOQJC010000156         8288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_4665_length_8288_cov_9.034860, whole genome shotgun sequence.
ACCESSION   DAOQJC010000156 DAOQJC010000000
VERSION     DAOQJC010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4665_length_8288_cov_9.034860"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..667)
                     /locus_tag="PLE39_17060"
     CDS             complement(2..667)
                     /locus_tag="PLE39_17060"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNE70095.1"
                     /translation="MIPSTANIACDSFVESLLRLLSLRDIETEEHTRRVAALSLDLAR
                     AYGKFSHAELFDIYQGALIHDVGKIGIPDAVLQKRGSLTPEEEAQIRMHPIYAYELFR
                     TLPCFEPILDIAYCHHEKWDGSGYPRGLRGEQIPLMARLFTIADVWDALTSERPYRAP
                     WPEEKVHDYILEQSGRYFDPAIVKLFISRYFRRSTPRKSAKKFIGYTTLQQKVNLFIG
                     EGR"
     gene            complement(727..1140)
                     /locus_tag="PLE39_17065"
     CDS             complement(727..1140)
                     /locus_tag="PLE39_17065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE70096.1"
                     /translation="MLPTILEGKKIFIIEDDPMSLSLISSCLLKHGARIEANALGFDI
                     IDHLVESMPVDLIILDVNLRKNLPGYQVLENIRSMPPLYQVPVVMVTATDWEIALPKA
                     RAAGANGFIAKPFNLAHFAAHIDRVLSGGQVWVTA"
     gene            complement(1140..4841)
                     /locus_tag="PLE39_17070"
     CDS             complement(1140..4841)
                     /locus_tag="PLE39_17070"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNE70097.1"
                     /translation="MKNQSTMTDKVILRAQALAWAGKHTQAIETASHELGRHNLKPKM
                     QMDLLDLRAESYIAIGKLDLAAADADAMTRLAKQGLSLSIQALNRKAIVQMRTGDLKS
                     AVKSASAALKIAQKSKQKRCISQSLLILSESQTRKGQLEESIVTAEKAIAIFQELNDL
                     SGAGRAYWCISTACFDLFRAENSRKAAQAALELCQQAGDQYGIGNAYNAYTYTDTDIA
                     ERLQHLHQAKAAYETAGYVERQTVALVNLAVVYSELGLYTHNRRLNEEAIRITGTMGA
                     RLLFIYALGNIIDTEIKLGELQSAQRRIKEYVKLVPTLGDPTQETSLAQALGDIAFAE
                     GNFKIAIQHYQSGLRISAQTNLGRENVFLTEIGKTHIASRDSATALKTTTKATNLHRE
                     QSYAKPDGFPTQAIWWRHVQALLANKKIKEAREALDRAYDLLLEGIQNIPDVGLRRNY
                     LNKVALNRELLQYWLKDASKRKLSKERTCAYLNIESNPREPFKRLTNTSLRLNALHTV
                     EEIKNFLVEEATELSGGERVVLILENDGKREAANSLLPHGEDARTFPASIGRYLTATR
                     LTHTTQLILPKKQGLSRIIAPLITQNKLIGYIYVDMDSTYGVFNETDRDMIGMLANQA
                     AIAIDNASLVIGLEQKINERTHQLQERIYELQIINSFQQNLAAEMDFQSIVSFVGDKL
                     REVFNTSDLGINWYDEKSNLLHYLYAYEHGVRLNVPPQAPRPGGLFERMTRTRQPFIL
                     NNAADYKKMNVPAIPGTDQSKSSIAVPIISNDRVLGFIATENHQRENAFGESELRLLT
                     TIAASLGSALENSRLLDETQRLLKESNTLADVGRDISSSLETKTVLENINSYASNLLK
                     GSMSAIFIPEKDGDSFRAIAVVGHNAEELRNEVIASGEGVLGDIARNKSAEIVNNIDN
                     DPRAVMITGTENVPDEHLLAVPLLADDELKGLMAVWRAGKNMSFTHNELTFLTNLARQ
                     AVIALKNSQLFTEMQEARATAEQADKAKSAFLANISHELRTPLNAIINFTELVAMGTM
                     GPINIEQEEALGYSLSSSKHLLQLINDVLDISKIQAGKLTLFVENDVNLHVEIQDTLK
                     MVEPLLHKHAELYGHDVKLIRDIDKGLPIIACDRRRIKQVLLNLLSNAVKFTQKGSIT
                     LSAKRHEDHIAFAVMDTGQGISQDQQKQIFEPFTQALEGTKHVEGTGLGLPITKSLIE
                     AHGGSIWLESTPGEGTSFFFKIPTTIQAG"
     gene            complement(4883..5290)
                     /locus_tag="PLE39_17075"
     CDS             complement(4883..5290)
                     /locus_tag="PLE39_17075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE70098.1"
                     /translation="MIKDPVFLYVEDDPYSIKVMRMIFEKVMRIPTLIVFEGSDNFMQ
                     NLLELNTPPDVFLLDIQMKPYNGFELLHMIRTDRRFRASKIVALTASVMGEEVDQLKR
                     SGFDGAISKPIHITSFPGLINRVLNGENIWFVS"
     gene            complement(5322..5744)
                     /locus_tag="PLE39_17080"
     CDS             complement(5322..5744)
                     /locus_tag="PLE39_17080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE70099.1"
                     /translation="MTITSTYHLENKRIFLIEDDVLNLAIINRVLINSKATVFQNYNS
                     IGIVTHIIQHLPIDLILLDIMLRRGVSGMDVILELKNNIHTANIPVVAVSALDPEIAI
                     PQAQRAGFNGFIGKPINSMSFSQDIAAIIHGEQRWVAS"
     gene            complement(5875..6627)
                     /locus_tag="PLE39_17085"
     CDS             complement(5875..6627)
                     /locus_tag="PLE39_17085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE70100.1"
                     /translation="MENLQKFDSIISLFTKLHQGQHTPPVGEWKRRQVPNPSAKSPAV
                     QILIVDDHRYVHDVISKIMVNVPDMTIAGHAANGQEAIQFCQTHQPDIILMDVVMPVM
                     DGLDASRTIHSLYPEVKILVLSSFQDHESVYALLKNGAVGYITKNSLTSELIQIIRTT
                     ATGKTVFSPEVMEQLLFSSQNPQMIYKFNLTDRELEILVLLADGMLNQQIAKKLTISL
                     STVKFHKTNIFQKLGVKTRSEALVVAVKNNLI"
     gene            complement(6605..7633)
                     /gene="fni"
                     /locus_tag="PLE39_17090"
     CDS             complement(6605..7633)
                     /gene="fni"
                     /locus_tag="PLE39_17090"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNE70101.1"
                     /translation="MKKVAPIDQRKADHIKINLEQDVRSALTTGLEKYRFIHEALPEL
                     DLNRIDTTLSLFGKKLASPTLVSSMTGGTSEAETINMRLAEAAQECGIAMGVGSQRAA
                     IEHPEQAATFQVRRVAPDILLFANIGAVQFNYGYGIDQCRKAIEMIEADGLYLHLNPV
                     QEAVQDAGDVNWVGLAKKIEEVCKKLEVPVIAKEVGWGISEHTARILADCGVQAIDVA
                     GAGGTSWSQVEMHRAPDEFTRQLAATFVGWGIPTAESIVGVKRAVPEMTIFASGGIKD
                     GLDIAKCVALGASLGGMAGHFLKAAAISTENVIETMELVKRQIEVTMFATGAGTLASL
                     RNGKLAKV"
     gene            7730..7972
                     /gene="acpP"
                     /locus_tag="PLE39_17095"
     CDS             7730..7972
                     /gene="acpP"
                     /locus_tag="PLE39_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNE70102.1"
                     /translation="MSDTFSELKDIIKDLLGADEAKITPEARFREELEADSLDLVELI
                     MAFEDKFGTEISDEDAQKITTVGEAVAYIDAHRGSK"
     gene            complement(8067..>8288)
                     /locus_tag="PLE39_17100"
     CDS             complement(8067..>8288)
                     /locus_tag="PLE39_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNE70103.1"
                     /translation="SEEEKAAEYIAKKMTKPVAAFIAGQTAPPGKRMGHAGAIIEGGA
                     GTAADKIKALEAAGVKVAKHPEEIPTLLQ"
BASE COUNT         1870 a         1853 c         2180 g         2385 t
ORIGIN      
        1 ttcaccttcc ttcaccaatg aataaattga ccttctgctg caatgtggta tatccgatga
       61 attttttagc ggatttgcgt ggggtagatc ggcgaaagta gcggctgata aacagcttga
      121 cgatggcagg gtcaaagtac ctgcctgatt gttcgaggat atagtcatgt actttttctt
      181 cgggccaggg tgcgcggtag gggcgttccg aggtgagggc gtcccacaca tccgcaatgg
      241 tgaagaggcg ggccatcagc ggaatttgtt cgccccgcaa gcctcgtgga tagccgctac
      301 catcccactt ctcgtgatgg cagtaggcaa tgtcgagaat cggctcaaag catggcaggg
      361 tgcggaacag ttcataggca taaattggat gcatgcggat ctgcgcctcc tcctcagggg
      421 tgagggatcc ccttttttgc agaacggcat cgggtatccc tatttttccc acatcatgga
      481 tcaaagcgcc ctgatagata tcaaacaact cggcatgact aaatttccca taagcccttg
      541 caaggtcaag tgagagggcg gcgacacggc gggtatgttc ttcagtttcg atatcacgta
      601 gtgagagcag ccgtaacagg ctttcgacga atgagtcgca ggctatattt gccgtacttg
      661 gaatcatgaa tttttccaat ctatgtatgg gaggggtgtg gaacataccc agaccaccga
      721 ccttttttat gctgttaccc atacctggcc tcctgataat acgcggtcga tgtgtgcggc
      781 aaaatgggcc agattaaatg gttttgcgat aaacccgttt gcgcctgctg cccttgcctt
      841 tggaagtgca atttcccaat cggtggcagt gaccatcaca actggcactt gataaagtgg
      901 cggcatggag cggatattct ccaaaacctg atacccaggc aggtttttgc ggagattgac
      961 atctaaaatg atcaagtcca caggcatgga ttcgacgagg tggtcgataa tgtcaaaccc
     1021 taatgcattg gcttcgatgc gggctccatg tttaagcagg caggaactga tcaaactaag
     1081 gctcatcggg tcatcttcga tgatgaagat tttttttcct tcaagaatgg tgggcaacat
     1141 caaccagcct gtatggtggt ggggattttg aagaaaaatg atgtcccttc acctggtgtg
     1201 ctttcgagcc agatacttcc accatgcgcc tcaatcaggc ttttggtaat cggcaacccc
     1261 aggccagtgc cttccacgtg tttggtccct tccaaagcct gggtaaatgg ttcaaagatc
     1321 tgcttctgtt ggtcttgcga aataccctga cctgtatcca tgacagcgaa ggcaatatgg
     1381 tcttcatgtc gttttgcgct taatgtaatg cttccttttt gcgtgaactt caccgcattg
     1441 gaaagcaggt tgagtaacac ttgtttgatt cggcggcgat cgcaggcaat gatcggcagg
     1501 cctttatcta tatcgcggat cagcttcacg tcatgtccat atagttctgc gtgtttgtgc
     1561 agcaagggct caaccatctt taatgtgtct tgaatttcga catggagatt tacatcgttc
     1621 tcaacgaaga gggtgagttt gccagcctgt atcttgctga tatccagaac atcgttgatc
     1681 aattgcaata gatgtttcga actgctcaag gaataaccaa gagcctcctc ttgctcaatg
     1741 ttgatcgggc ccattgtgcc cattgcaacc agttcagtga agttgataat ggcattcagc
     1801 ggagtgcgta gttcatggct gatgttggcc agaaaagcgc ttttggcttt atcagcttgt
     1861 tctgctgtgg cgcgggcttc ctgcatttca gtaaacaact gcgagttttt caatgcaatg
     1921 accgcctgcc ttgccagatt ggtaaggaag gtaagttcat tatgagtgaa actcatattt
     1981 ttacccgccc gccacacggc catcaggcct ttcaattcgt catccgccaa cagtgggaca
     2041 gccagcaggt gctcatcggg gacattttcc gtgcctgtaa tcatcaccgc acgtggatca
     2101 ttgtctatat tatttacgat ctcagcagat ttatttctgg caatatcgcc caatacacct
     2161 tcccccgatg cgataacctc atttcgaagt tcttcggcat tgtgccccac tacagcaatt
     2221 gcgcggaagc tatccccatc tttttctggg atgaagatcg cgctcatgct tcccttgagc
     2281 aggttactgg catacgagtt aatgttctca aggacggttt tggtctcaag gctggaggaa
     2341 atgtctcggc ctacatcggc gagggtattg ctttccttca acagccgctg ggtttcatcg
     2401 agcagacgag agttttcgag ggcagaacca agcgaggcag cgatcgtggt cagcaggcgc
     2461 agttcagatt ctccaaaggc attttcacgc tgatggtttt cggtggctat gaatccaagc
     2521 acacggtcat tgctgatgat gggcaccgca atgctggact tgctttggtc agtgcctggt
     2581 atggcgggta cattcatctt tttgtagtct gctgcattgt tgaggatgaa cggttggcgg
     2641 gtgcgggtca tcctttcaaa caggccgcct gggcgtggag cctgcggagg aacattcaac
     2701 ctgacgccgt gttcataggc gtataaatag tggagcaggt tcgatttttc atcgtaccaa
     2761 ttgattccaa ggtcggaggt gttaaacact tctctaagtt tgtcacccac gaaactcacg
     2821 atggattgga aatccatttc agcggccaga ttttgctgga aggagttgat gatttgcagt
     2881 tcatagatgc gttcctgcag ttgatgggtg cgctcgttga tcttttgttc aaggccaatg
     2941 acgaggcttg cattatctat tgcgatggct gcctgatttg caagcatgcc gatcatatcg
     3001 cggtcggttt cgttgaagac accgtaggtc gaatccatat ccacgtagat gtagccgatg
     3061 agtttgtttt gggtgatgag tggggcaatg attcggctga gtccctgctt tttggggaga
     3121 atcagttgag tggtatgggt gaggcgggta gcggtgagat atctgccgat cgaagcgggg
     3181 aatgtccgcg cgtcttctcc gtgtgggagt agggaattcg ctgcttcgcg ctttccgtcg
     3241 ttttcaagga ttaagacaac acgttctcca ccgctgagtt cggttgcttc ttccacaaga
     3301 aaatttttga tctcttcaac agtatgtaaa gcattaagac gcaggctggt attggtgaga
     3361 cgtttgaacg gttcgcgggg attcgattcg atattaaggt aggcgcaggt acgttctttt
     3421 gagagttttc gcttgctggc atctttcagc caatattgca gcaattcacg attgagcgcc
     3481 actttgttga ggtagttacg acggaggcct acatctggaa tattttgaat accttccagc
     3541 aacaggtcat aagcgcggtc aagcgcttca cgagcctctt ttattttttt gttggcaagc
     3601 agtgcctgca catgacgcca ccagatcgcc tgtgtgggga aaccgtctgg tttggcatag
     3661 gattgctcac gatgcaggtt tgtggctttt gtggtggttt tgagtgctgt ggcggagtct
     3721 ctgcttgcaa tgtgggtttt gccgatttcg gtgaggaaaa cattttccct gcccaaattt
     3781 gtttgcgcag agattctgag tccagattga tagtgttgaa tggctatctt gaaattgcct
     3841 tccgcaaagg cgatatctcc cagagcttgg gcaaggcttg tttcctgtgt tgggtcgcca
     3901 agtgtgggga cgagtttcac gtattctttt atgcgtcgtt gggcagattg gagttcgcca
     3961 agtttgattt ctgtgtcaat tatattgcca agtgcgtaaa tgaataataa tctggctccc
     4021 atggtgccag taatgcggat cgcttcttca ttcaggcggc gattgtgtgt atacaggcca
     4081 agctcggaat atacaacggc aaggtttaca agagcaacag tttggcgttc tacatagcct
     4141 gcggtttcgt aggcagcttt ggcttgatgc aggtgttgaa ggcgttcggc aatatctgta
     4201 tcggtatagg tgtaggcatt gtatgcgttg ccgatgccgt attgatcacc cgcctgttga
     4261 cagagttcaa gcgcggcttg tgcggctttg cgtgaatttt cagcgcggaa caggtcgaag
     4321 caggcagttg agatgcacca atatgcgcgg cctgcccctg aaaggtcatt gagttcctga
     4381 aaaatggcaa tggctttttc tgcggtaaca attgattctt ccagttgtcc ttttcgagtt
     4441 tgagattcgc tgaggatgag caggctttga gaaatacacc ttttctgttt tgatttttgg
     4501 gcaattttca atgcggcact ggcagacttg acagcacttt tcaggtcacc agtgcgcatt
     4561 tgaacgatgg ctttgcggtt gagtgcttga atggacaagg ataaaccctg ctttgccagc
     4621 cgtgtcatgg catctgcatc tgcggcggcg aggtcgagtt ttccaatggc aatgtaagac
     4681 tcggctcgca ggtcgagcag atccatttgc attttgggtt tcaggttgtg tctgcccaac
     4741 tcatgcgagg ctgtttcgat ggcttgtgta tgtttgcccg cccatgccag agcttgcgca
     4801 cgcaaaataa ctttatcggt cattgttgac tgatttttca tgacttcatc caatttctgt
     4861 aaatcaggaa gatgtgaact ttttatgaaa cgaaccagat gttttcacca ttcaggacgc
     4921 ggttgatcag acctggaaac gatgtgatat gtatgggttt tgaaatggca ccatcgaatc
     4981 cgcttctttt gagctggtcc acttcctcgc ccatgacact tgcggtgagt gccacaattt
     5041 tacttgctcg aaaccgtctg tctgtgcgaa tcatgtgtaa aagttcgaag ccattgtatg
     5101 gcttcatttg aatatccagc aggaaaacat caggaggcgt atttaactca agcaggtttt
     5161 gcatgaaatt atcactgcct tcaaaaacga tcaaggttgg aatgcgcatc actttttcga
     5221 agatcattct cataaccttg atggaatatg ggtcgtcttc cacatacaga aatacagggt
     5281 ctttgatcat gaatgttgta ttcctgaggc cgcccgacca gctagctggc aacccatcgc
     5341 tgttctccat gaataatggc ggcgatatct tgcgagaaag acattgaatt aattggcttt
     5401 ccgatgaatc cgttaaaacc agctctttgt gcctgcggga ttgctatctc cgggtcaagc
     5461 gcagatacag ctacaacagg gatattggcg gtgtgtatgt tatttttgag ttcgagtatt
     5521 acatccatgc cgctgacgcc cctgcgcagc atgatatcaa gcaggatcag gtcaatgggc
     5581 aggtgttgaa tgatgtgggt gacaataccg atgctgttgt agttttgaaa taccgtggcc
     5641 ttactgttga ttagcacgcg attaatgatc gccagattca aaacatcatc ctcgatcagg
     5701 aagatccttt tgttttcaag atggtaggtg ctggtgatgg tcataattct cctttgctgt
     5761 ttcgctgtta gtttcaaaac cctgatttgt caggcaactg cgcgatttta ttggcaggca
     5821 gggagattgg gtactgtctc tttaaaacga aaacctgtcc atttggacag gtagctagat
     5881 gaggttgttc ttgacggcga ccaccagtgc ctcggagcgt gtttttacac ctaatttctg
     5941 gaaaatgttt gttttatgaa acttcacagt ggaaaggctg atggtaagtt ttttcgcgat
     6001 ctgctgattg agcatcccat ctgccagaag aacgaggatt tcgagttcac ggtctgttag
     6061 gttgaatttg tagatcatct gcgggttttg ggaagaaaaa agcaattgtt ccatcacctc
     6121 tggggaaaat accgtcttcc cagttgcggt tgttctaata atttggatca gctcactggt
     6181 aagggagttt ttagtgatgt atccaactgc tccgtttttt aggagagcat atacactttc
     6241 atggtcttga aaactggaaa gcacaaggat cttgacctca gggtaaaggc tgtgaatggt
     6301 gcgagatgcg tcgagaccat ccatgacagg catgacaacg tccatcaata taatgtcagg
     6361 ttggtgggtc tgacagaatt gaatggcttc ctgcccgttg gcagcatgac ctgcaatggt
     6421 catgtctggg acattgacca tgatttttga aataacatcg tgaacatatc tgtgatcgtc
     6481 cacgattagt atttggactg ccggggattt tgctgaaggg ttaggcacct gtcttctttt
     6541 ccattctcct actggtggtg tgtgctgtcc ctgatgtaat tttgtgaaca gtgaaattat
     6601 actatcaaac ttttgcaagt tttccattgc gcaatgatgc caatgttcct gctcctgtgg
     6661 caaacatggt gacctcgatc tgccttttga ccagttccat ggtttcgatg acattctcag
     6721 tggagatggc cgctgccttg aggaagtgcc ctgccatgcc gcccagggat gcaccgaggg
     6781 caacacattt ggcaatatcg agaccgtctt tgatgccgcc actggcgaag atggtcattt
     6841 cgggcacggc tcttttcacg ccgacaattg attctgctgt gggaattccc cacccgacaa
     6901 aagtcgctgc cagttggcgg gtgaattcat cgggtgcgcg atgcatttcg acctgagacc
     6961 agcttgtgcc gcctgctcca gccacgtcaa tggcctgtac gccgcaatcg gccaaaatcc
     7021 tggctgtgtg ttctgaaatt ccccaaccca cttccttggc tataacgggt acctcaagtt
     7081 ttttgcaaac ctcttcgatt ttctttgcga gaccaaccca attgacatct cctgcatctt
     7141 gtacggcttc ctgcacaggg tttaagtgca gataaagccc atctgcttcg atcatctcga
     7201 tggctttgcg gcattggtca atgccataac cgtagttgaa ttgcacggcg ccgatattgg
     7261 caaagagcag aatatccggt gcaacacggc gtacctgaaa tgtggcagcc tgttccgggt
     7321 gttcaattgc agcgcgttga cttccaaccc ccatggcaat tccacattcc tgggcggctt
     7381 cagcgaggcg catgttgatg gtctctgctt cagaagtgcc gcctgtcatg gaactgacca
     7441 gggtggggga tgccaatttt ttgccgaaca ggctgagggt cgtgtcgatg cggttcaaat
     7501 ccagttctgg cagggcttcg tgaatgaatc ggtacttttc aagtccagtg gtcagtgcag
     7561 agcgaacatc ctgttctaag ttgattttaa tgtggtcagc ttttctttga tcaatgggcg
     7621 caactttttt cataaggtag tgttcctgag tatgatgatc cgaacccggc ttgacatgtc
     7681 aagtggacgg attacaatag gccagtcaaa atttgaacag gaggttgtca tgagtgacac
     7741 atttagtgaa ctgaaagata tcatcaagga tctgcttggt gcggatgagg cgaagatcac
     7801 acctgaagcc cgtttccgcg aagaattgga agccgattcg ctcgatctgg tcgagttgat
     7861 tatggcgttt gaagataaat ttggcaccga aatttccgat gaggatgccc agaagatcac
     7921 cactgtgggt gaggctgttg catacattga tgctcaccgc ggcagcaagt agaaactccg
     7981 caattactga atgagattat aaccttagag ccgcccgacg acgtggtcgc caggcggctc
     8041 tattgattct tgatagagat tgaaagctat tgcagaaggg tgggaatctc ttccgggtgc
     8101 ttggcaacct tgacgccagc ggcttcaagg gctttgattt tgtcggcggc tgtgcctgct
     8161 ccgccttcga tgattgcacc ggcatggccc atgcgttttc cgggaggggc ggtttgcccg
     8221 gcgatgaagg ctgcaacggg tttggtcatc ttctttgcga tgtattctgc ggctttttct
     8281 tcttcgct
//
LOCUS       DAOQJC010000157         8148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_4758_length_8148_cov_9.984925, whole genome shotgun sequence.
ACCESSION   DAOQJC010000157 DAOQJC010000000
VERSION     DAOQJC010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8148
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4758_length_8148_cov_9.984925"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..3423
                     /locus_tag="PLE39_17105"
     CDS             253..3423
                     /locus_tag="PLE39_17105"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HNE70104.1"
                     /translation="MKTQNRISPTVKALRGIVLLAVLFSMIQAQPASAQVTGQGLEIC
                     LLPGTVQLKQDESQALKSALRKADAEVEFNGAVCFAPIELREEGEWMFITLAGLTNKP
                     GGAWTISEDAVWSDNAIIRKTPGGMLASVAGTKAFSAALSSVPESIVPLEAKRVIDPL
                     SAYSPSPHAGAAFQFPFEAGYQVYYGTKGGGVHTGEFSTVLGGTSMAVDLMSDGITAS
                     NHAPNRLLAVAPGTVVKKCSDATKNSTYLIIDDGTDKFLYAHIRSSTAPPLWAVVNVA
                     GTILGELVPGSFSDGYCGYANQTAGFFHVHLEFLNRSSLTLSDWTLDTATEKWQRGAE
                     VWSAGEWKQVGGSVTGCSQVSYTGVILYENANCNVETAGAMKAFPAAITWTDIAVADP
                     IQNKASSIFVSSGWSAKVFEKSIAEGGGSWRCVTDRLSDLSTSYYTNGDTSRKMNDDI
                     SSIQVYADGNCGSGGTSDWTALYFNGHDHWLDTSNFNNQMCQETITSSGLDKNYTSSA
                     PCNNGVANDWVAEYNTTVNFASGNYVFKAENDDGLKLWVNGVSVLDRSAAQGLSYACP
                     ARSLSGNVPIRAILNEETGDARIKIAWTTDVNVCNLPGAFGKVGPVNGATAQPNNPTL
                     SWNASVDATEYFYCIDTSNDTVCDTGWQSVGNVTSQAISGLAVDKTYYWQIRANSAGG
                     ETFADGNTWWSFKTQPSNMLINPGFEDGTTTPTAWNKDAFVLANSTFEWSIDFFHSGS
                     RSVKIISATANDARWTQTVTVQPNTDYKLSGWILTNDVAHTAETNDAGANLSILDGTV
                     NHSTPSLFGDNPWTFVSLTFNSGANTQVTVAARLGMATGSTTGTAWFDDLKLEPATSA
                     PTFGDVPLDHPQYAYIQALWDNGYTAGCSTDPLLFCPDTILDRAQSAVFMLRGQLGST
                     YAPPAAPWNTFGDDWTGFEWAEPWAEGMWQEGLTQGCQASPLLYCPSTQLPRVEASVF
                     GLRMKYGVNYTPPTATGTLFADFPSTDPSYWAIDWAEQAYLDGLLPACGTDSVSGKPM
                     FCPSQLVDRGWGAYLIVQAKGLLPTP"
     gene            3850..5223
                     /locus_tag="PLE39_17110"
     CDS             3850..5223
                     /locus_tag="PLE39_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNE70105.1"
                     /translation="MKKLHRSFRLLVILFVLVNLLPSYNSLAAPLAADYPTDPTADIS
                     WSAGTNGVADVQAAFNQARAVESGQLGISLPALTLPSQADWDALSDGEKSLWLINRER
                     SDRGVDPLEDVELNVTSVAQTYADFLLDNDAWGHTEDGRDPWERLDSNPAIQACHDFL
                     GVSENLAVFVTSGTSISLPIERAVYMWMYDDSGSGWGHRHAILWSSYTDSHGSSGSEG
                     FLGIGRASGGPYQGPFSQPWLHAELIVMNVFDPCAGYSVPQATFGDVPVDHPQYKYIQ
                     ALYDGGYTAGCSTSPLMFCPDTIMDRAQAAVFMMRGQFGSGYTPPPAPWDTFADDWTG
                     FEWAEPWAEGMWQEGLTGGCRQDPLMYCPETDLSRVEATVFGLRMKYGVNYEPPLATG
                     KVFADLTDPTYWGAGWAEQAYADGLLPECGFDNETGKPYFCPLDLVNRAWGAYLIVKA
                     KNLLPAP"
     gene            5457..7025
                     /locus_tag="PLE39_17115"
     CDS             5457..7025
                     /locus_tag="PLE39_17115"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNE70106.1"
                     /translation="MNKRSYLPVVLIMLIVMVFGGHTNAYASNPAAAKSARVEVNGVS
                     TPCTTFKACDDLLGPDATLYISGEWNEQLRVTESGTAGHPIRVIGTDAIIKVDKTEVS
                     GIALTGNYINLSGFEVIGGDSFGIYITGQVIKVENNIVHDSVLENGTAGNCRQGSWGS
                     AIKVREAMYVEVRNNQVYHSCGEGIALRGTYVLAEYNTVWDNFSVQLYVDNSPYTTAQ
                     NNTVKCTPGFPTPRQPVGIAIGEERFSGWGNQRHDNKVLNNYVDGCADGIVSWEGEMS
                     TAKFIDSVISGNTVVNGNGRAIGLWSKNQNVLIENNTIYSEVFIKYPEGVTSINNTVI
                     GSTPSIFSDVPATYWAVSYIESLYNAGITGGCSTSPLLYCPTSSVTRAQMAVFLLRGI
                     HGSGYAPPAATGAVFGDVSTSTFAAAWIEQLAAEGITSGCGNGNYCPDATVTRAQMAI
                     FLLRSKYGSAYVPPVATGVFPDVPVGSFAADWIEQLAAESITSGCGGGNYCPNSSVTR
                     DQMAVFLVKTFNLP"
     gene            7099..7950
                     /locus_tag="PLE39_17120"
     CDS             7099..7950
                     /locus_tag="PLE39_17120"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE70107.1"
                     /translation="MKTQNQSIPQFDLGGNGPSLHFLHANGYPPECYLPLFELIKTQY
                     HLFGMKLRPLWPDSSPKEISDWKPLSDDLLRFLSNRGTDPVIGVGHSIGGIVTLRAAL
                     RAPQKFRAIILLDPVLFVPNFILMWNIVRALGLGNKAHPKIGGALKRRREFDNLDLIF
                     RGYRTREVFKYMSDESLRAYILGITKEKQGGGFELAYSPEWEARIYLTGLRDLDIWHR
                     LPKLEVPTLIIRGAETDTFLEAAAMLVKQKQPKVKIESLPESTHILPLEYPQKVFEIM
                     QTFLNDL"
BASE COUNT         1924 a         1981 c         2135 g         2108 t
ORIGIN      
        1 agccgatgtt ctgcccaagt caactggtgg atcgcggctg gggtgcgtac ttgatcgtca
       61 aagccaagaa cctgccgttg ccgtaagttt gccagactag atcgaataaa aaagggcagt
      121 cactgaaaaa gtggctgccc ttttgtcatg caaatacgat gaatctctcc caatttctga
      181 cttataaaat agatgcaggt attgcgcatt tgtgcgcaga agtttgttat tttgccagaa
      241 tggaaggttc atatgaaaac acagaatcga atttctccaa cggtgaaggc cttgcgtgga
      301 attgtgcttt tggcggttct cttttccatg attcaggcac agcctgccag cgcgcaggtc
      361 acagggcagg gattggaaat ctgcttgttg cctggtacgg tgcagctgaa gcaggatgag
      421 agtcaggcgc tcaagtccgc gctgcgaaag gcggatgcgg aagtggaatt caacggcgcc
      481 gtatgctttg ctccgatcga attgcgcgag gagggtgagt ggatgttcat cacccttgca
      541 gggttgacga acaaaccagg cggcgcttgg acgatctcag aggatgcggt ctggtcagat
      601 aacgcgatca ttcgcaaaac cccgggcgga atgctggctt cggttgcggg tacaaaagcc
      661 ttttctgcgg ctctttcttc ggtgccagaa tcaattgtcc cgttggaagc gaagcgagtg
      721 atcgatccat tgtctgccta ctcgccgagt ccacatgcgg gggcggcatt ccaattcccg
      781 tttgaggcgg ggtatcaagt ttattatggg acgaagggcg gcggtgtcca cacgggtgag
      841 ttttctacgg tgctgggcgg cacgagcatg gcagtggatt tgatgtcgga tggaattacg
      901 gcttcaaacc acgcgccgaa tcgtttgctg gcggttgcgc cgggcacggt ggtaaagaaa
      961 tgttcagatg ccaccaagaa ttccacttat ctcatcattg atgatggcac cgataaattt
     1021 ttgtatgccc acattcgttc atccactgcg ccgcctttgt gggcggtggt gaatgtggca
     1081 gggacgatcc tgggtgagtt ggttccaggt tcgttcagcg atgggtattg cggttacgcc
     1141 aaccagaccg caggtttctt ccatgttcat ctggaattcc tgaatcgttc ttcgctcacg
     1201 ctcagcgatt ggactctcga tacggccact gaaaaatggc agcgcggtgc cgaggtgtgg
     1261 agcgctggtg agtggaagca ggtgggtggc agcgtaacgg gatgcagtca ggtttcatat
     1321 acgggcgtga tcttgtatga gaacgccaac tgtaatgtgg aaaccgcagg ggcgatgaaa
     1381 gccttcccgg ctgcgatcac gtggacggat attgctgtgg cggacccgat ccaaaataaa
     1441 gcatcttcga tcttcgtctc ttcgggctgg tcggctaaag tatttgagaa atccatcgct
     1501 gaaggcggcg gctcgtggcg atgtgtgaca gatcgtcttt ctgacttgag cacaagttat
     1561 tacaccaatg gcgatacatc gcggaagatg aacgatgaca tctcgtccat tcaagtttat
     1621 gcggatggaa actgcggcag cggcgggacc tcagactgga ctgccctgta tttcaatggg
     1681 catgatcatt ggctggatac ttccaacttc aacaaccaga tgtgccagga aaccattacc
     1741 agcagcggcc tcgataagaa ctacacttcc tccgctccct gcaataatgg tgtggccaac
     1801 gattgggttg cggaatacaa caccacggtg aattttgcct ctggaaatta tgtcttcaaa
     1861 gccgagaatg atgacgggct caaactgtgg gtgaatggcg tcagcgttct tgaccgaagc
     1921 gccgctcaag gcctttcgta tgcctgccca gcccgcagct taagtgggaa cgttcccatc
     1981 cgtgcaatcc tgaatgaaga aacaggcgat gcccgcatca agatcgcatg gacgacggat
     2041 gtcaatgttt gtaacctgcc gggcgcgttt ggcaaggttg gccctgtcaa cggtgccact
     2101 gcacaaccta ataatcccac cttgagttgg aatgccagtg tggatgcaac ggaatatttc
     2161 tactgcatcg atacttcgaa cgacactgtt tgcgatacgg gctggcaaag cgttggcaat
     2221 gtgaccagtc aggcgatcag tgggttggca gtggataaga cctactattg gcagatccgc
     2281 gcgaacagtg caggtggtga gacctttgcc gatggcaata cctggtggtc ttttaaaacc
     2341 cagccttcca atatgttgat taacccaggc ttcgaagatg gcacaaccac cccaactgcc
     2401 tggaacaagg atgcctttgt gttagccaac agcacctttg agtggagcat tgatttcttc
     2461 cactcaggca gtaggagcgt gaagatcata agcgccacag ccaacgatgc gcgctggact
     2521 caaactgtaa cggttcagcc aaatacggat tacaaattat ctggctggat cttgaccaat
     2581 gatgttgccc acaccgctga aacgaacgac gctggagcca atctctccat tttggatggt
     2641 acggtcaacc actcaacccc gtcactgttc ggcgataacc cgtggacttt cgtgtctttg
     2701 acattcaact ctggtgcaaa cacgcaagtg acggttgccg cgcggcttgg catggctaca
     2761 ggctcaacaa caggcaccgc ctggtttgat gacctgaaat tggaaccagc aacaagtgcg
     2821 cccacctttg gcgatgtgcc gcttgaccat ccgcaatatg cctatattca ggcgttgtgg
     2881 gataacggct acaccgcagg atgcagcacg gatccgctgt tgttctgccc cgatacgatc
     2941 ctggaccgtg cccagtctgc ggtgttcatg ctgcgcggac aacttggctc aacctatgct
     3001 ccgcctgccg caccgtggaa taccttcggc gatgattgga ctgggttcga atgggccgag
     3061 ccgtgggcgg aaggcatgtg gcaggaggga ttgactcaag gttgtcaggc cagcccgttg
     3121 ctgtattgtc cttccacaca gcttccgcga gtggaggcca gcgtatttgg tctgcgcatg
     3181 aaatacggag tcaattacac cccgcccaca gccacaggga cattgtttgc cgatttccca
     3241 tccacagacc cgagttactg ggccattgac tgggccgagc aagcctacct tgatggtctg
     3301 ctccccgcct gtggcaccga tagtgtcagt ggcaagccga tgttctgtcc aagtcaactg
     3361 gtggatcgcg gctggggtgc gtatctaatc gtgcaagcca agggattatt gccaacacca
     3421 taatccctgc ttttctcctt tgagttgcag cgcgagacct ctgggtcttg cgctgcacgt
     3481 tttaacgggt ttggttatgg atgttcttgt aactattcca cattttcatc gttcattttt
     3541 tacgagtaga actcttgcat aagtccattt ctttaccacc aaggcacaaa gtctcgaaga
     3601 ctcaaagaaa aaaagcttag tgcctttgtg actttgtggt ctacaacact ttacttatgc
     3661 aagaggtcaa gtgttttttt gagtgcaaca gaacgtccat tgtgaaattc atattgaaat
     3721 aaatttttat ttttattgaa gaataaaatt attttgcgat gtacgcttaa caaacctgtg
     3781 ggtttgggaa tttataaaaa atggtatatg tagccatcat tgttgcagaa atatccacgg
     3841 gagttaagta tgaaaaaact acatcgctca tttcgccttt tggtaatcct ttttgtgctt
     3901 gtgaatctat tgccttccta taattcatta gccgctccgt tggcggctga ttatccaacc
     3961 gatccaacag cggatatcag ctggtccgca gggacaaatg gtgtggcgga tgtgcaggct
     4021 gcattcaacc aagcgcgggc tgtggagagt ggtcagcttg gtatctcatt acctgcattg
     4081 accctgccca gtcaagcgga ctgggatgcc ttgagcgatg gcgaaaaatc cctgtggctg
     4141 atcaaccgtg aacgaagcga ccgtggtgtc gatccgcttg aagacgtgga actgaatgtc
     4201 accagtgtgg cccaaaccta tgctgatttc ctactcgata atgatgcctg ggggcacact
     4261 gaagatggcc gtgatccgtg ggagcgcctt gattccaacc ctgctattca ggcttgccat
     4321 gatttcctcg gtgtttcaga gaatcttgcg gtttttgtca ccagcggaac gagtatttct
     4381 ttaccgatcg agcgcgctgt ttacatgtgg atgtatgacg acagcggttc tggctggggg
     4441 catcgccatg ccattctctg gtcttcttat actgatagcc atggctcctc tgggagcgaa
     4501 ggctttctcg gcattggccg tgcatctggc ggaccgtatc agggtccatt ttcacaaccc
     4561 tggttgcatg ctgaattaat tgttatgaat gtttttgatc catgcgctgg ttattctgtt
     4621 ccgcaagcca cctttggcga tgtgccagtg gaccatcctc aatataaata tattcaggct
     4681 ttatatgatg gcggctatac ggcagggtgc agcaccagtc cgctgatgtt ttgccccgat
     4741 acgatcatgg atcgtgccca ggcggcagtc ttcatgatgc gcgggcagtt tggctcaggc
     4801 tatacacctc cacctgcacc atgggatacc tttgctgatg attggacggg ttttgaatgg
     4861 gcagagcctt gggccgaagg gatgtggcag gaaggcctca caggtgggtg tcgacaggat
     4921 ccgctgatgt attgtcctga gacagacctt tcacgtgtgg aggcaacggt cttcggtctg
     4981 cgcatgaagt atggagttaa ctacgagcca ccattggcaa cgggaaaggt tttcgccgac
     5041 ctcaccgatc ccacctattg gggcgcaggt tgggcagagc aagcctatgc agatggcttg
     5101 ctcccagaat gcggttttga taatgagacg ggcaagccat atttctgtcc gttggatttg
     5161 gtcaaccgtg cctggggggc gtatctcatt gtcaaggcca agaacctgct tccagcgccg
     5221 taagatcgtt tcaacatggg cggaatcatg ccaaaaggcg attccttaca ttggggagcg
     5281 gtcatttgac ggggttttcg tccaactaac aaaattgtag gtttttttga aatctttttg
     5341 agcaccaatg gatactgttt aatgtatcat tttcatatca aaacgccgag gcggctcgat
     5401 ttgcttcggc tttaattttg gacatcgaat ttttctatat ttaataagga aagaaaatga
     5461 ataaaagatc atacctccca gtcgtcctca ttatgctgat cgtcatggtg tttggcgggc
     5521 atacaaatgc gtatgcctcc aaccccgctg ctgccaaaag cgccagggtg gaagttaatg
     5581 gtgtatccac accatgtact acattcaaag cgtgtgacga tttgctcggc cccgatgcca
     5641 cactctacat ttcaggagag tggaacgagc aattgcgtgt caccgagtcg ggtactgcgg
     5701 gtcaccccat tagagtcatc ggtactgatg cgatcatcaa ggtcgataag accgaggtga
     5761 gcggaatcgc cctaacaggg aattacatca acctctctgg ttttgaggtc attggaggcg
     5821 actcctttgg catttacatc acagggcagg ttattaaagt ggaaaacaac attgtccacg
     5881 atagtgtgct tgaaaatggc acggctggca attgccgcca gggcagttgg ggtagtgcca
     5941 ttaaggttcg tgaagcgatg tatgtggaag tacgcaataa tcaggtttat cacagctgcg
     6001 gtgaagggat tgccctgcgc ggaacctatg ttttggcaga atacaacacc gtgtgggata
     6061 acttctcagt acagctttat gtggataact cgccatacac cactgcccag aacaataccg
     6121 tcaaatgcac cccaggcttc ccaacccccc gtcaacctgt tggaattgcg ataggcgaag
     6181 aacgctttag cggctggggc aatcagcgcc acgataacaa ggttttgaat aattatgttg
     6241 atggctgtgc agatggcatt gtttcatggg aaggtgaaat gtccactgcc aaattcatcg
     6301 actcagtgat ttctggcaat acggttgtaa atggaaatgg acgtgccatc gggttatggt
     6361 caaagaatca gaacgtgttg attgaaaaca atacgatcta ctccgaggtg ttcataaaat
     6421 atcccgaagg cgtaacttcg atcaataaca ccgtcattgg ctcaacgcct tccatctttt
     6481 cagatgttcc agccacatat tgggctgtaa gttatatcga aagtttgtat aacgcaggca
     6541 ttactggtgg atgctccaca tcgccgcttc tgtattgccc aacttcctct gtgacccgtg
     6601 cgcaaatggc ggtcttttta ctgcgtggaa ttcatggcag cggctacgct ccacctgcag
     6661 caactggagc agtctttggt gatgtttcca ccagcacctt tgctgctgca tggatcgagc
     6721 aattggcggc tgaaggtatc accagtggat gcggtaacgg aaactactgt cctgatgcca
     6781 ccgtcacccg cgcacagatg gctattttcc ttttgaggag caagtacggt tctgcgtatg
     6841 tgccgcctgt ggcaactggt gttttcccgg atgttcccgt tggctccttc gcagcagact
     6901 ggatcgaaca attggccgct gaaagcatca ccagtggatg tggcggcgga aactattgtc
     6961 ccaactcgtc ggtaactcgc gaccaaatgg ctgtattcct tgtgaagacc tttaatcttc
     7021 cataggttta aactaatttg caaatttatg aagccttcac aacttgtgaa ggcttcatat
     7081 tatttataat caaaacaaat gaaaactcag aaccaatcaa tcccccagtt cgatttaggc
     7141 ggtaatggcc catctttgca cttccttcac gccaacggct atccccccga atgttacctg
     7201 ccgctctttg agcttatcaa aacccaatac catctttttg gaatgaaact tcgcccgctt
     7261 tggcctgatt caagcccgaa agagatttcc gactggaagc ctctctccga cgacttgctg
     7321 cgcttcctct ctaaccgtgg aactgatcct gtaattgggg tgggtcattc catcggaggc
     7381 attgtcactt tacgcgccgc cttgcgcgct ccgcagaaat ttcgcgccat cattttgctg
     7441 gacccagtct tgttcgtgcc gaactttatc ctgatgtgga atattgtccg tgcattgggg
     7501 cttggaaaca aagcccaccc aaagattggc ggcgcactaa aacgcaggcg tgagttcgac
     7561 aatctggatc tgatctttcg ggggtaccgt acccgtgagg ttttcaaata catgagcgat
     7621 gaaagtctgc gggcttatat tttgggtatc acaaaagaaa aacagggtgg tggttttgaa
     7681 cttgcttatt ctcctgaatg ggaagcgcgt atttacctca caggcctgcg cgacctcgat
     7741 atctggcaca gattacccaa actcgaagtg ccgaccctca tcatccgcgg cgctgaaacg
     7801 gatacgtttc tggaggcagc cgcaatgctt gttaagcaga agcagcccaa ggttaagatc
     7861 gaatcgttgc ctgaatccac gcatatcctt ccgttggaat atccgcaaaa ggtctttgag
     7921 atcatgcaga catttctcaa tgatctgtaa accctcgaaa ggtcactcga agcttcggaa
     7981 ttccaaaaaa acctacttga ctcgcaacct tttttcttgt atgctgtttt aacgttagaa
     8041 caattttttg aacgattgtt caaaacctca caggagaaga gaaaatgtcc catatcaagt
     8101 tgagttggcg caacatcatc atcgcggcga ccctgcccgc cgtgatgg
//
LOCUS       DAOQJC010000158         8140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_4760_length_8140_cov_8.097341, whole genome shotgun sequence.
ACCESSION   DAOQJC010000158 DAOQJC010000000
VERSION     DAOQJC010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8140
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4760_length_8140_cov_8.097341"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PLE39_17125"
     CDS             complement(<1..985)
                     /locus_tag="PLE39_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70108.1"
                     /translation="MSTKTVFRAFSVLFVFVFLLAQVGGAGAISPMANSVVLTPGRAI
                     ASWPSGVPAGEKRPVVVFLPGWGGSGAVDAAVSAQNTNLVNQGYVTLAIGFNSPSDWS
                     SDIDVQTADGLDKLCADASIPANCDAIILDGESYGGAQNYYVIEYIRSNGYGGKALGF
                     VSEDAGYAAPGNLIDPVTGEFQRTGLADTASYGVAMIQNLGDSTFPVDDCTWGNCGAR
                     TLSDAHQAQGDTNVFSICPSGGEHGTRGYADWDAWVVSAIKTLFHSVYGVPTFTGYTA
                     PTISVGNTCLSSGGSSTFVDVPTTHPLYKYIQALYDAGYTAGCSSSPMMFCP"
     gene            complement(1119..2531)
                     /locus_tag="PLE39_17130"
     CDS             complement(1119..2531)
                     /locus_tag="PLE39_17130"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HNE70109.1"
                     /translation="MKTIKFLWVTLVASMLLFALMLPGGKAKSNGMQQAPNGVGGTAW
                     NLIWSDEFDGSAVNSNDWNFNVGGEGWGNNELEYYTDGQNVTVANGELVITAKNENGG
                     YACWYGPCEYTSTRMTTKAKQEFQYGRVEARMKLPNGQGLWPAFWMLGSNFDQVGWPA
                     SGEVDVMEHINSEDIVYGTMHWDAGGYASYGCVSTDVLASVDVTQYHVYAIEWDTEKI
                     VWYVDDTQYCEANIRDSINSTEEFHAPFYILLNLAVGGNWPGAPDAGTAFPASMAVDY
                     VRVYSADVTSIFTDVPPTYWAWNFIERLYNAGITGGCSTAPLMYCPEATVTRAQMAIF
                     ILRGMHGSAYVPPAATGTVFADVPLGAFAADWIEQFAAEGVTAGCGGGNYCPDATITR
                     AQMAIFLLRGEHGSAYVPPVATGAVFGDVPAGSFAADWIEQLAAEGITSGCGGGNYCP
                     DSPVTRAEMAVFLVRAFSLP"
     gene            complement(2633..4564)
                     /locus_tag="PLE39_17135"
     CDS             complement(2633..4564)
                     /locus_tag="PLE39_17135"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNE70110.1"
                     /translation="MSAKSPVFRILLGMLVFSLIFSAVDVDVRAAPLADPVLDWSTFL
                     GGNGGDYGQAMAVDVTGSIYVVGYSDSDWGSPIRAFDSANDAFVAKFDSSGNLLWNTF
                     LGGGGDDVGINIVTSGNSIFVAGYSSSDWDCAPLTCTMDDYTLGYDAFVAKLDSSGNL
                     NWITFLGGNGSDFGRGIDVDFSGNIYVVGGSDGTWGSPIQSHAGGGYDGFAAKLDSTG
                     ARTWNTFQGSGGDDQTWGVALDVSGNAYLAGYSNDTWGSPVIAYDADYDGFAAKLNSN
                     GALIWNTFLGGNGEEHSHAIALDGSGTIYVTGYSTDTWGSPVRAYTSNDVFAAKLTSS
                     GALTWNTFLGGAGADSGYALALSGADIYVTGYSTDTWGSPDRAYTSGFDSFAAKLNTS
                     GVLTWNTFLGGSGSDFSYGVAQSGSGLFVGGHSDDTWGTPLQAYDTAVDSFLVKFAGE
                     TTPPPASPIFADVPFDHWANPWVETLYNAGVTGGCATAPHLIYCPDDAVTRAQMAIFI
                     LRSMHGGAYLPPAATGAVFGDVPADAFAADWIEQLAAEGVTTGCGDGNYCPEAAVTRA
                     EMAIFLLRGEYGGAYTPPAATGTVFGDVPSDSFAADWIEQLASEGITVGCGDGNYCPD
                     MNVTRAEMAVFLVKTFHLP"
     gene            complement(4715..7705)
                     /locus_tag="PLE39_17140"
     CDS             complement(4715..7705)
                     /locus_tag="PLE39_17140"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNE70111.1"
                     /translation="MRNVQLKKMFSKLLSTIILATMICSTFLSGNIELVRALGGRDAS
                     SAQEDGNSLYYFANGNRIQLTPSLNWISVKFTSDDPVAQSAAVGGFGAMFGPLDQARP
                     ILFGNIALLPLKANITEQALMEGIQSLRADKENFSQVNPVFQTPDAEMALTDEFIATF
                     PSDFSMEDIDAVNAFHNVELIDAILGQDNTFLLRVAAGTDVLAMANLYQQSGVAIHSA
                     PNFVRIVKINPTEGQSGQHVGPMAGTNDTYYPSQWHLNNNQQYGSGTTNDADIDAPEA
                     WDRTTGNSSVVIAVIDEGVDLIHEDISPKLVAGYDATGLGSGGGPSGDDAHGTNVGGL
                     AAASSNNGFGVAGVCQKCSIMPVRIAYSDAFGFWVTNDATIANGITWAYQNGASILNN
                     SWGGGLEATVINTAIANAKTLGRGGKGSVVIFSAGNSNSSVSYPAYLDSVIAVAASNL
                     CDQRKKPIADVCNGYEYWWGSNYGSPVDISAPGVWLYSTDITGAAGYASGNYFADMNG
                     TSGAAPIVAGVAGLILSLNSNLTAIEVQTILQNTADDVNGGGYDVEMGYGRVNAYRAV
                     QAVTTSGYTISGDAGIGGATLTYTDGTVKTATADSSGLYSFQVPSGWSGTVTPSKSGY
                     EFSPTSRTYTSVGADQTAQDYTASVPIINLLQDPSFEAYTPNTYWLETSTNFGTPLCT
                     IADCTNGAGTAGPRTGSVWAWFGGTTKDEAASLTQTVVFPTGTANLRFYLWIGRADSG
                     RGAADQFTVTVDGVVVFTADATEMSAYSSYTMVNLDVSTFADGAAHDIGFSANTSGQM
                     VNFNLDDITLAEAIPTFSDVPLDYWARSYIERLYNSGITRGCGSNPLTYCPLDSVTRT
                     QMAIFILRGIHGSGYTPPEASGTVFSDVTTSTFGAAWIEQFALEGITSGCGSGKFCPN
                     DYVTRAQIAIFLLKGKHGSTYVPPAATGDFTDVPVGSFAADWIEELAAEGITSGCGGG
                     NFCPSQNVTRDQMAVFLVKTFNLP"
BASE COUNT         2205 a         2280 c         1815 g         1840 t
ORIGIN      
        1 cggggcagaa catcatcggg ctgctggagc agcctgcggt gtagcctgca tcgtacaatg
       61 cttgaatata tttatacaac gggtgcgtcg tcggcacatc cacaaaagtg gaagacccgc
      121 ccgagctcag acaagtattg ccaaccgaga tcgttggagc ggtatatcct gtaaaagtag
      181 gcacaccata aacggaatgg aaaagggtct tgatggcaga aaccacccat gcatcccagt
      241 cagcataacc gcgtgtgcca tgctcgccgc ctgaagggca aatgctaaag acattggtat
      301 cgccctgcgc ctgatgtgca tcgctcaatg tacgggcacc acagttgccc catgtacaat
      361 catccacagg gaaggtgcta tcgcccaaat tctgtatcat ggcaacacca taggaagcag
      421 tatcagccag acccgtccgc tggaattcgc cggtgacggg atcgatcagg tttcccggtg
      481 cagcatagcc cgcatcttca gaaacgaaac ccaaagcctt tccgccatat ccattgctgc
      541 ggatgtattc aatcacataa taattctgcg caccgccgta agattcaccg tccaaaataa
      601 tggcatcaca attggcaggg atggaagcat cggcacacag tttgtccaaa ccatcggcgg
      661 tttgtacatc aatatcactg ctccaatctg aaggcgaatt gaacccgatc gccagagtaa
      721 catacccctg attcacaaga ttcgtattct gggcagatac ggccgcatcg accgctccgc
      781 ttcctcccca cccgggcagg aacaccacaa caggccgttt ctccccggca ggaacaccag
      841 acggccaact ggcaattgct ctaccgggcg tcaacacaac gctgttcgcc atcggagaaa
      901 tcgcgcccgc cccaccaact tgtgccagta aaaagacgaa gacaaataaa acagaaaaag
      961 cacggaagac ggttttggta gacatacaga taatcccttt caaattgcac tgtgcaattc
     1021 acaacacctt cactgaattg catcttgtat caatatacag ggtttttaag gccaattcaa
     1081 gaccgaaata gtcctattac tgcatcaatg caacctgatc atggcaggct gaacgctctc
     1141 accaaaaata ccgccatctc agcgcgtgtg acggggctat cggggcaata gtttccgccg
     1201 ccacacccgc tggtgatgcc ttccgcagcc aactgttcga tccaatccgc agcgaatgaa
     1261 ccagcaggga catccccaaa gaccgcaccc gttgcaactg gaggaacgta ggcactgccg
     1321 tgttcaccgc gcaaaaggaa gatggccatc tgcgcgcggg tgatggttgc atcgggacag
     1381 taattcccgc cgccacaacc tgcggtcact ccctccgccg caaactgctc gatccaatcg
     1441 gcagcaaacg ccccgagagg gacatctgca aagaccgtcc ctgtcgccgc tggcggtaca
     1501 taggcgcttc catgcatacc ccgcaaaata aaaatggcca tttgcgcacg tgtaactgtg
     1561 gcttccgggc aatacatcaa tggggctgtg ctgcatccac cggtaatgcc cgcgttatac
     1621 aaccgctcaa tgaaattcca tgcccaataa gtgggcggca cgtctgtaaa aatggaggtc
     1681 acgtctgcag aatacacccg gacataatcc actgccatac tggcagggaa ggccgtgccg
     1741 gcatcgggtg ctccaggcca atttccgcct actgcaagat tcagcaaaat gtagaagggt
     1801 gcgtgaaatt cctctgtgct gttgatgctg tctcgaatat tggcctcaca gtattgggta
     1861 tcatccacat accagacgat cttttccgta tcccactcaa tggcatagac gtggtattgg
     1921 gtcacatcca cactggcaag cacatccgtc gaaacacagc cgtatgaggc atagccgcca
     1981 gcatcccaat gcatggttcc atacacgata tcttcagaat tgatatgctc catcacatcc
     2041 acttcgcccg aggcaggcca gcccacctga tcaaaattgc ttcccagcat ccaaaatgca
     2101 ggccataaac cctgcccatt cggcaatttc atccgcgctt cgaccctgcc atattggaat
     2161 tcctgtttgg ccttggtggt catacgagtg gaggtatatt cacaggggcc ataccagcag
     2221 gcatacccgc cattctcatt tttagctgtg atcaccaact caccattggc aaccgtcacg
     2281 ttttggccgt cggtgtaata ctccaattca ttattccccc agccttcccc acccacattg
     2341 aaattccagt cattgctgtt cactgccgaa ccatcaaatt catcgctcca gatcagattc
     2401 caggcagttc cacccacacc attcggtgcc tgctgcatgc cattgctctt tgcttttcct
     2461 ccaggcaaca tcaaggcgaa caacaacatt gaggcgacaa gcgtcaccca tagaaatttt
     2521 atggttttca tggtcacatc catttcaaca aggttttctt cacaaaaaca agagccacag
     2581 caggaaactc tgcggctctt gtttcggaca atggattgga gaacgtaact tcctatggca
     2641 aatggaacgt ctttaccaaa aagaccgcca tctcagcacg ggtcacattc atatccgggc
     2701 agtaattacc atctccgcag ccgacagtaa taccttccga ggcgagttgt tcgatccaat
     2761 ctgccgcaaa ggaatcggag ggaacatcgc caaagaccgt acccgttgca gcaggaggag
     2821 tataagcacc gccatactct ccacgcaaca ggaagatcgc catctcagca cgggtcacgg
     2881 ctgcctccgg acaataattg ccgtcgccac agccagtggt caccccttcg gccgccaatt
     2941 gctcgatcca atctgccgca aaagcatcag cagggacatc gccaaagact gcccccgttg
     3001 ctgctggagg caggtaggca cctccatgca tgctgcgcaa gataaaaatt gccatttggg
     3061 cgcgggttac agcatcatca ggacaataaa tcaagtgcgg cgccgtggca caaccgccag
     3121 taacgcctgc attgtacaac gtctcaaccc atggattagc ccagtgatcg aatggaacat
     3181 ctgcaaagat cggagaggct ggcggcgggg tcgtttcacc tgcaaatttc acaaggaaag
     3241 aatccacagc ggtatcgtaa gcctgtaacg gggttcccca agtatcgtca ctatgcccac
     3301 caacaaataa accactgcca ctctgcgcaa caccgtaact aaagtcgctg ccactaccgc
     3361 caaggaaagt attccaagtc agtacaccgc ttgtatttag ttttgcagcg aaactatcaa
     3421 acccgctggt ataggcacga tctgggctcc cccaggtatc ggtgctgtaa cctgtaacat
     3481 aaatatccgc cccactcaaa gcgagagcat acccagaatc agcgcctgcc cccccaagga
     3541 aggtattcca tgtcaaagcc ccggaggagg tcagctttgc ggcaaataca tcattagagg
     3601 tataagcccg taccggactg ccccaagtgt ctgtgctata ccctgttacg taaatcgttc
     3661 cgctcccatc caaggcaatg gcatgactgt gctcttctcc gtttccaccg aggaaggtgt
     3721 tccagatcaa ggcaccgttg ctgttcagct tcgcagcaaa accatcatag tctgcgtcat
     3781 aagcaatcac tgggctgccc caggtatcgt tgctatagcc tgcaaggtag gcatttccgc
     3841 tcacatccaa agcaacgccc caggtttgat catcaccccc gctaccttga aacgtattcc
     3901 aggttcgcgc accggtcgaa tccaattttg cagcaaagcc gtcatacccc ccgccagcat
     3961 gagactggat cggactaccc caagtgccat cacttccccc aaccacataa atatttccgc
     4021 tgaaatccac atcgattcca cgcccaaagt cactgccatt tcccccaaga aaggtgatcc
     4081 aattcaagtt accggaagag tcgagcttgg ccacgaaggc atcataacca agggtatagt
     4141 catccatggt gcaggtaagg ggcgcacaat cccaatcact gctgctatac cccgctacaa
     4201 aaatgctgtt cccacttgta acgatgttta taccaacatc gtctcccccg ccgcctagaa
     4261 aagtattcca aagcagattt cccgacgaat cgaattttgc tacaaaagca tcatttgcgg
     4321 aatcaaaagc acggatcgga ctcccccaat ctgaatcgct gtacccaact acataaatac
     4381 tccccgtaac atccactgcc atcgcctgcc cataatcgcc gccatttccc ccgaggaagg
     4441 tactccaatc tagaacagga tcagccaacg gcgcagcacg aacgtcaaca tccactgctg
     4501 agaatatgag ggaaaaaaca agcatcccca aaaggatacg gaaaacgggt gatttcgcgg
     4561 acatgtgaaa cgctcccttc cgaaataaat tgtgggcaat acaaacgctt ttatcactat
     4621 acaaggtttt taagacaaat acaacaactc gatgttcaaa ataaaaaagg cgcctccaaa
     4681 agtatggaga cgcctttgat aagaaggtcg tttactacgg tagattgaag gtcttcacaa
     4741 ggaaaactgc catctggtcg cgggtaacat tttgacttgg gcagaaattt ccgccgccgc
     4801 atccgctggt gataccttca gcggcaagtt cctcgatcca atcagctgcg aaggatccaa
     4861 cggggacatc ggtgaaatca cccgtggcag caggcggtac atacgttgag ccatgtttcc
     4921 ccttgagcag gaaaatagcg atctgtgcgc gggtcacata atcatttgga cagaattttc
     4981 cagagccgca tccagaagtg atgccttcca atgcaaattg ttcgatccag gcggcgccaa
     5041 aagtgctggt tgtgacgtct gaaaatacgg ttccgctggc ttccggggga gtatagccgc
     5101 tgccatgaat accgcgcaaa atgaaaatgg ccatctgggt gcgtgtgaca gaatccaacg
     5161 ggcagtaggt caatgggttg gaaccgcatc cacgtgtgat ccctgagttg tacaggcgct
     5221 caatataaga ccgcgcccaa taatcaagcg gcacatcaga aaatgtggga atggcttcag
     5281 ccagcgtaat atcatccaga ttgaaattga ccatttgtcc agatgtgttg gctgaaaaac
     5341 caatatcgtg cgccgcgccg tcggcaaatg tactcacatc cagattgacc attgtatagc
     5401 ttgagtaagc actcatctcg gttgcatctg cagtaaagac cacaacacca tccacggtaa
     5461 ctgtgaactg gtctgctgct cctctgcctg agtcagcccg cccgatccaa agatagaacc
     5521 gaaggttggc agtgccagtg gggaaaacca cggtttgcgt caacgaagca gcttcatctt
     5581 ttgttgtacc gccaaaccat gcccatacag aacccgtccg cggcccggca gtaccggcac
     5641 cgttggtaca gtctgcaatg gtacacaagg gagtgccaaa gtttgttgag gtctccagcc
     5701 aatatgtatt gggggtatat gcttcaaagc tcggatcttg taacagattg ataattggca
     5761 cagaagccgt gtaatcctgg gctgtctggt ctgcgccaac actcgtataa gttctgctcg
     5821 tgggtgaaaa ctcatacccc gacttgcttg gtgtcaccgt accagaccaa cctgaaggga
     5881 cctggaacga atacaagcca ctactatctg cagtagcggt tttcactgtt ccatcggtat
     5941 aggtgagggt agcgccaccg atgcctgcat cgcctgaaat ggtgtaccct gacgtggtca
     6001 ccgcctgaac agcacgatat gcattgacac gcccatatcc catttcaaca tcatatccgc
     6061 caccattcac atcgtctgct gtattttgga gaattgtctg aacttcgatg gctgtcaaat
     6121 ttgaatttag cgaaagaatc aacccagcca caccagccac aatgggagcg gcaccagatg
     6181 ttccgttcat atcagcgaaa taatttccag atgcataacc agccgcgcct gtgatatcgg
     6241 ttgagtataa ccagacgcct ggcgcagaaa tatccaccgg gctgccgtaa ttgctccccc
     6301 accagtattc atatccattg cagacatcag caattggttt tttgcgctgg tcgcacaaat
     6361 tcgatgcagc caccgcaatc acactatcca gataagcagg gtaggaaact gatgaattac
     6421 tgtttcccgc cgaaaatatg actacagaac cttttcctcc acgacccagc gtcttcgcat
     6481 tggcaattgc cgtattgatg accgtggctt caagtccgcc gccccaacta ttattcaaaa
     6541 ttgaagcacc attttgatat gcccaggtaa ttccattggc aatagtggca tcgtttgtga
     6601 cccagaatcc aaaggcatca ctataagcaa tacggacagg cataatactg cacttttgac
     6661 atacacctgc tacaccaaac ccgttgttgc tacttgcggc tgcgaggccg cccacatttg
     6721 taccgtgagc atcgtcgcca gaaggcccac ccccactgcc aagcccggtt gcatcataac
     6781 cggcaaccag tttgggacta atatcttcat ggatcagatc gacaccttca tcaatcacag
     6841 caatgactac agacgaattg ccggtggtgc gatcccatgc ttctggagca tcaatatcag
     6901 catcgttcgt cgtccctgaa ccatattgct gattgttatt caaatgccat tggctcggat
     6961 aataagtatc attcgttcca gccattggtc caacatgctg tcctgactgc ccttccgtcg
     7021 ggttgatctt cacgatgcgg acaaaattcg gtgcggaatg gatcgcaaca ccgctctgct
     7081 gatataaatt cgccattgcc aatacatccg tcccagcggc aacacgcagc aaaaaggtat
     7141 tatcctgtcc aagaatggcg tcaatcagtt caacattgtg aaatgcattg acagcatcaa
     7201 tatcttccat gctgaaatcc gatgggaagg tggcgataaa ttcatccgta agcgccattt
     7261 cggcatccgg ggtttggaag acaggattca cttgtgagaa gttctcttta tcagcgcgca
     7321 aggattggat tccttccatg agcgcttgtt ccgttatatt tgctttcaaa ggcagaaggg
     7381 cgatattgcc gaataaaatt ggacgcgctt gatccaacgg accaaacatt gcaccaaaac
     7441 ctccgactgc cgcagattga gcgaccggat cgtcacttgt gaatttcacc gatatccagt
     7501 tcaaggatgg agttaattga atgcgatttc cgttggcaaa ataatacaga gagtttccat
     7561 cctcctgggc tgatgatgca tccctcccac ccaaagcccg caccaattca atattccccg
     7621 acagaaatgt ggaacagatc atggtggcta aaataattgt agaaagcagc tttgaaaaca
     7681 ttttcttcaa ttgaacgtta cgcataactg ttctccttaa gtggcaacca tttttgagaa
     7741 aatcgtttaa gtaaagcata ccatgattgc tacaattaac aaatcatttc aaatggtcgc
     7801 atttcgtgat aaaaccagcc gaatagctat ccaatgattt ttacattaca acttttgttt
     7861 tttaaagtga atcaatatta ttcttttccc ctatataagt caacgactcc atcaatcctt
     7921 gaatcgaatt agactgacgg agtcgtgaat ccttgaaact ggtttaccta cggcaaattg
     7981 aaggccttca caaggaaaac tgccatctgg tcgcgggtga cattttggtc tgggcagaaa
     8041 ttcccgccgc cacatccact ggtgatgcct tcagcggcaa gctgttcaat ccaatcggct
     8101 gcgaaggaac caacggggac atctgcaaat atgcccgtgg
//
LOCUS       DAOQJC010000159         7768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_5127_length_7768_cov_7.952807, whole genome shotgun sequence.
ACCESSION   DAOQJC010000159 DAOQJC010000000
VERSION     DAOQJC010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7768
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5127_length_7768_cov_7.952807"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..846)
                     /locus_tag="PLE39_17145"
     CDS             complement(112..846)
                     /locus_tag="PLE39_17145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HNE70112.1"
                     /translation="MEQYLQKDYTGGAFELYGPAHLVALGVIVIACFVLIRFGKSWNE
                     QQRRNFRYFMAVFLIVDELSWHAWAAYWGIWTVQTMLPLHICSVMVWASAYMLFTKNY
                     NIYEMAYFLGIGGATQALLTPDANQYGFPHYRAFQTFIAHGLLVIIPIYMTLVEGYRP
                     TLQSFKRVFIWTNIYMIIVFFINLAIGSNYLFIAHKPEFPTLLDMLAPWPWYIIELEA
                     IGFAIFFLIYVPFLLKDRRAKQIVTA"
     gene            complement(906..1643)
                     /locus_tag="PLE39_17150"
     CDS             complement(906..1643)
                     /locus_tag="PLE39_17150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HNE70113.1"
                     /translation="MGQYFSLVYEGEPISLFGRGHLIALTIIVLFCFSFLYFRKVWGE
                     KEKKIARWVLAILIVVNESALHIWSAYWGIWNIQTMLPLHMCSVMIWASAYMLVTRNY
                     SIYEMVYFLGLGGAMQAVLTPADAAAYNFPHFRIMQTFTAHGLLIGIAIYMTVVEGFR
                     PTLQSFKRVFIWTNLYMIVIFFLNLAIGSNYLFVAGKPEFPTLLDMLAPWPWYIIELE
                     AIGFAIFFLLYVPFLIKDWKAKQATTA"
     gene            1845..2897
                     /locus_tag="PLE39_17155"
     CDS             1845..2897
                     /locus_tag="PLE39_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70114.1"
                     /translation="MKQRNLYIVLAVIVLAVVAVVYFSMNSKPAAPAQLTEEVKLTEM
                     PVTEIAMSGPASTPEAEFSGLAWYGENLIMLPQYPNIFDESGDGQLFYLPKAELSLYI
                     GGARGAPIEPRPIKLIAPNLTDQIRNYQGFESIAFSGDRVFMTIEAGEGTDMMGYLIS
                     GTINPDLSTLTLDTTKLAEIPPQAKSENHSDEAILIMDDKILTFYEVTGKDIVSKPVV
                     HVFDFDLNPQGTLPIDNLEYRLTDIAMGFGNTIWGINYFFPGDEDLMPSSDPILERFG
                     TGETHAKFPQVERLIRYQYSDAGLTLVNSKRPIYLTLTEDIRNWEGLVVLDTAGLIMV
                     TDKFPRTILAFVPIPR"
     gene            complement(3199..3660)
                     /locus_tag="PLE39_17160"
     CDS             complement(3199..3660)
                     /locus_tag="PLE39_17160"
                     /inference="COORDINATES: protein motif:HMM:NF016184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HNE70115.1"
                     /translation="MFDQIYNFLRYSEDLSSSGMPTAEQLSEAAKHKVQVVINLAPHD
                     VPNAIPNEGQLVESLGIEYINIPVNWGTPTEDGLDKFMDAMDERNGKVIHVHCEANFR
                     ASAFVAMYRILRLGWKEEDAFEIMHKIWDEDAYPVWKLFIEGMVSQSGKNL"
     gene            complement(3660..4136)
                     /locus_tag="PLE39_17165"
     CDS             complement(3660..4136)
                     /locus_tag="PLE39_17165"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNE70116.1"
                     /translation="MTQLSPTAQKIQNLLNSLGYNYTVIEHTESTRTAQEAADRAGCE
                     LGQIVKSLIFRGKTSGKPILVLTSGVNRVDEKRISGYAGEPISRADADFVRAVTGYAI
                     GGVPPIGHNEKMETYLDEDFLQYGVVWAAAGTPNAIFELSLNDLQKMTTGKVTQVK"
     gene            4157..4849
                     /locus_tag="PLE39_17170"
     CDS             4157..4849
                     /locus_tag="PLE39_17170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE70117.1"
                     /translation="MPTQEEIYKTEGDKYEALIAREDHQGNILKSLREITPLEHRFVL
                     DIGAGTGRLACLLAPHVSHVRAFDISEEMLRVCREKFLASGLVNWQVDVADHRQLPVD
                     DASADLVVSGWSVAYLAVWNPDSWRAELEKWLGEMKRVLRPNSFIVLFESLGTGNETP
                     IRLEHLKDYYPWLEEVGFQHKWIRTDYKFESLEEAEFLSRFFFGDELGDKVKQNNWVI
                     LPECTGVWWKKM"
     assembly_gap    5139..5238
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5239..5688)
                     /locus_tag="PLE39_17175"
     CDS             complement(<5239..5688)
                     /locus_tag="PLE39_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70118.1"
                     /translation="MLGNLSDRFYKWAKGWLIFVLFILDGFFAGFLLPLIQGLMQDDR
                     GGVQPLDLMLFATPEKIFAMIERYGEFGRPFYRNVELTVDIIYPIVYLFFFGLFISWL
                     FQRGFSANSAMRKYNIIPLGAWFFDLLENIIIVILLSIFPTQPAALAW"
     gene            complement(5768..6232)
                     /locus_tag="PLE39_17180"
     CDS             complement(5768..6232)
                     /locus_tag="PLE39_17180"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE70119.1"
                     /translation="MTQVLYGPRLGREGQTRVGCSAVIFDKDRKKALLTQRTDNGRWC
                     VPGGALEAGESAAEACEREVWEETGLKVRATRLIGVYSNPDQLVVYPDGNKAFFVVLS
                     FEAEIIEGELGLSNETTAFGYYSLEEMDGLPMHGQHRQRVEDALLGGAALMK"
     gene            complement(6384..7232)
                     /locus_tag="PLE39_17185"
     CDS             complement(6384..7232)
                     /locus_tag="PLE39_17185"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNE70120.1"
                     /translation="MTNRTISHIIEPQLVVEGAGVLLRRSVAPRASNEYDPFLLFDHF
                     AFNNPLEGPIRGFPMHPHRGIETVTYMLDGSVNHRDSLGNAGTIGAGDVQWMTSGRGI
                     LHEEMPKRGQSGNIYGFQLWVNLPAAQKMGQPRYQEVNSSTIPVVEKDGATIRLVAGE
                     VNGVRGPVTEIAASPLYMDVKLAPGATFTYPIPSGHTVLAYVFEGVGEFAGEVVESVS
                     MVVFNDDGEQIEVKSDAASSGVQFMLIAGAPFKEPIVPYGPFVMNTVEEIQQTIQELR
                     NGTFIK"
     gene            complement(7263..7619)
                     /locus_tag="PLE39_17190"
     CDS             complement(7263..7619)
                     /locus_tag="PLE39_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70121.1"
                     /translation="MSNDPFIEKALAAIRAMDFEIIEGLRDEIKPAHVPTLLQTWNRD
                     LPWDVKDAYAALLMDQTGGTVKEFMEDALGSPTAETRAYAICILKNDFNLFSDFLVDG
                     GVDASKVDAAIAAYRS"
BASE COUNT         1961 a         1869 c         1893 g         1945 t
ORIGIN      
        1 attctccttt gggtttggtt aatgggattt tacgataaag gccttgcgta tgcaataaaa
       61 caaaagacct aaaggatccc tttaagtctt ttgtggtttg gtattttgtg ttcaggcagt
      121 gacgatttgt tttgcccgcc tgtctttaag aagaaatgga acataaatga gaaagaagat
      181 cgcaaatccg atcgcttcca attcaataat gtaccagggc cagggggcca gcatatccaa
      241 cagggtgggg aattcgggtt tgtgcgcgat gaagagatag ttgctgccga tggctaggtt
      301 gataaaaaag acgatgatca tgtagatatt tgtccaaatg aagactcgtt tgaacgattg
      361 cagagtgggg cgataccctt caaccaaggt catgtaaatg gggatgatca cgagcagtcc
      421 gtgtgcgatg aaggtttgaa aggcacggta atgtgggaaa ccatattgat ttgcatctgg
      481 agtgagaagg gcctgcgttg ctccgccgat gccaagaaaa taagccattt cgtaaatgtt
      541 gtagtttttg gtgaaaagca tgtaagccga ggcccacacc atcacactgc aaatatgcag
      601 aggcagcatg gtttgaacgg tccaaatgcc ccaatatgcc gcccatgcgt gccaacttaa
      661 ttcatccacg atcaggaaga ccgccatgaa atagcggaag tttcgtcttt gctgttcatt
      721 ccagcttttt ccaaaacgga tcaagacaaa acaggcgatg acgatgacgc ccagcgcaac
      781 cagatgggct gggccatata actcgaaggc gccgcctgtg taatcttttt gtaaatattg
      841 ctccatgctc agctcctttt tgtgaattgc tgttaaaacg cttttgcatg gcgcaagttt
      901 cggttttagg cagtggtcgc ctgtttggcc ttccaatcct tgatgagaaa cggcacatac
      961 aacaggaaga agatcgcaaa cccgatggct tccagttcga taatgtacca gggccagggg
     1021 gccagcatat ccagcagggt ggggaattca ggcttgcccg ccacaaaaag ataattactg
     1081 ccaatggcaa gattcaggaa gaaaatgacg atcatgtaga gattcgtcca aatgaaaacc
     1141 cgtttgaaag attgcagggt gggtcggaat ccctccacca cggtcatgta aatggcgatt
     1201 ccgatcaaca atccatgcgc ggtgaaagtc tgcatgatcc gaaagtgtgg aaagttgtac
     1261 gcggcggcat cggctggggt caataccgcc tgcatggctc caccaagccc aagaaaatac
     1321 accatttcgt agatgctgta atttctcgtc accagcatgt aagccgaggc ccagatcatg
     1381 acactgcaca tgtgcagcgg cagcatggtt tgaatattcc atatccccca atatgccgac
     1441 cagatatgca aggccgactc attgaccacg atcaatattg ccagaaccca gcgggcaatc
     1501 ttcttttcct tttcccccca cactttacgg aaatacaaaa atgaaaaaca aaatagaaca
     1561 atgatcgtca gggcgatcag gtggccccgc ccgaaaagtg agattggctc cccttcataa
     1621 accagtgaaa aatactgtcc catatcaact ccctttctcg cagttcatct gccttgcgta
     1681 tttttaccac aggatataaa cttcgacgcc cgtaaattaa agcatcttta aagcggcgtt
     1741 acaaaagacg ggatgcgaat ataatcttac gaaacacaaa ctggttgccg ccttgcgcaa
     1801 agaaccacag gcggccaaat ctaaaacatg ttggaggaat tgttatgaaa caacgaaatc
     1861 tgtatattgt attggctgtc atcgtactgg cggttgtcgc agtggtttat ttttccatga
     1921 attcaaaacc tgccgcacct gcccagttga ccgaagaagt caaactcact gaaatgcctg
     1981 ttaccgagat cgccatgtcc ggacctgcat ccacacctga agcggaattc tccgggctgg
     2041 cctggtatgg tgagaatctc atcatgctgc ctcaataccc gaatatcttc gatgaaagtg
     2101 gtgatggtca gctcttctac ctgccgaaag cggagcttag tctctacatc ggtggagcac
     2161 gcggcgcccc cattgaaccc cgccccatta aactgatcgc ccccaacctc accgaccaga
     2221 tccgcaatta tcaaggcttt gagtcgatcg ccttctccgg tgaccgagtc ttcatgacca
     2281 ttgaagcggg cgaaggaacc gacatgatgg gatacctcat ctctgggacc atcaaccccg
     2341 atctttccac actgacactt gatacaacaa aactggctga gatcccccct caggcaaaat
     2401 ctgaaaacca ctccgacgaa gctattttga taatggacga taaaatcctg actttttatg
     2461 aggtcaccgg gaaagatatc gtctccaagc ctgttgtgca tgttttcgat tttgacctca
     2521 atccacaggg aaccctgccc atcgataacc tcgaatatcg cctgaccgat atcgccatgg
     2581 ggtttgggaa caccatttgg ggcatcaatt acttcttccc cggcgacgaa gacctgatgc
     2641 cttcaagcga cccgattttg gagagattcg gtacgggtga aacccatgcc aaattcccgc
     2701 aagtggaacg tttgatccga tatcaatatt ctgatgctgg gcttacactg gtcaatagca
     2761 agagacccat ctatctgacc ttgaccgaag atatccgtaa ctgggagggt ctggttgttt
     2821 tggatacggc tggtctgatc atggtcaccg ataaattccc cagaacgatt ttggcgtttg
     2881 tgccgattcc aagataggat ttttgctcca caactacatg attcacctca tacctaggaa
     2941 tcagccgcga atttttgcaa aagactgaaa gattaacgac ggagccacgg agacgtagaa
     3001 aaaacgaata aaaactccgt atttcagtgt ctccgtggtt caaatagctt ttcaaagaca
     3061 ttccttgcaa cttatgcaag atacggacga gttataaaaa aagccgaccc tataaaatgg
     3121 atcggctttt tttgtaaatt cgcgaaatct gcggctaaag aattgaatga ttaatttcgg
     3181 gatgtttcat gtttgctttt ataagttttt tccgctctga ctgaccatgc cctcgataaa
     3241 caacttccac acggggtagg cgtcctcatc ccagatcttg tgcatgatct cgaacgcatc
     3301 ttcttccttc catccaaggc gcaaaattct atacatggcc acgaacgcac tggcgcggaa
     3361 gttcgcttca cagtggacat ggatgacttt cccgttgcgc tcatccatgg catccatgaa
     3421 tttatccagc ccatcctcgg tgggtgtgcc ccaattgacg gggatgttga tgtactcaat
     3481 gccaagcgac tccacgagtt gaccttcatt tgggatcgcg ttggggacat catgcggcgc
     3541 aaggttgatg acgacttgaa ccttgtgttt tgctgcctcg cttagctgtt cggcggtcgg
     3601 cattccactg gaagatagat cttcggaata acggagaaag ttgtaaatct ggtcaaacat
     3661 catttcacct gcgtaacctt tcctgtggtc attttttgca ggtcgttcaa cgataattca
     3721 aaaatggcgt tgggggtgcc tgccgctgcc caaaccaccc cgtattgcag aaaatcctca
     3781 tcgagataag tttccatttt ttcattatgg ccgatgggtg gcacgcctcc gatggcatac
     3841 cccgtcactg ctcttacgaa atcagcatcc gcgcggctga tgggttcgcc tgcatatccg
     3901 ctgatgcgtt tttcgtctac acggtttacg ccgctggtca gcaccaggat cggttttccg
     3961 ctggttttgc cgcgaaagat gagcgatttg acgatctggc ccagttcaca gccagcccga
     4021 tcggcagctt cctgcgctgt gcgcgtggac tcggtgtgtt cgatcacggt gtagttatat
     4081 ccgagtgagt tgagaaggtt ttggatcttt tgggcagttg gagaaagttg tgtcattcgc
     4141 caattataat tggctcatgc ctacacaaga agaaatctac aaaaccgaag gggataaata
     4201 cgaggcgttg atcgcccgcg aggatcatca gggaaatatt ctgaaatctc tgcgcgagat
     4261 cacgcccctt gaacaccgct ttgtgctgga tattggagcg ggtacgggac ggctggcctg
     4321 tttacttgct ccgcatgttt cgcatgtccg cgcgtttgac atttcagagg aaatgttgag
     4381 ggtttgccgt gagaaattcc tcgccagtgg gttggtcaac tggcaggtgg atgtggccga
     4441 ccatcgtcag ctgcctgtgg atgatgcctc tgccgacctg gttgtctctg gttggagcgt
     4501 ggcttatctc gcagtttgga atcccgattc gtggcgggct gagttggaaa aatggctggg
     4561 tgaaatgaaa cgcgtcttaa ggccgaacag cttcatcgtc ctgtttgaat cgttgggcac
     4621 gggcaacgaa actccgatca ggctggaaca tctcaaagat tattatccct ggctggagga
     4681 agtcggattc cagcataagt ggatccgaac cgattacaag tttgaatcgc ttgaagaagc
     4741 cgaattcctc tcgcgatttt tcttcggcga tgagttgggt gacaaggtga agcaaaacaa
     4801 ctgggtgatt ctgcctgagt gtacaggcgt gtggtggaag aaaatgtaag gataaaagag
     4861 ggccgcgata caaatatcac ggccctcttt ttgtgtatca atcagggctt acgcaaaact
     4921 ccgatttatg accgcacacc acggtctgcg gtcaattatc aaaagaagcc ttttatgcct
     4981 gaactttgaa tttgtttttt gccgccatga ccaagccgat caggatggat aagatactgg
     5041 cgcctgcaaa gagccatttg accgtggtca gcaggatcaa aacccaggcg agggcggcag
     5101 gctgagtggg gaaaatggat aacaagatga cgataatgnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnncc aggcgagggc ggcaggctga gtggggaaaa tggataacaa
     5281 gatgacgata atgatgtttt ccagcaggtc aaaaaaccac gcgcccagcg gaatgatgtt
     5341 gtatttgcgc atcgcgctgt ttgcggaaaa tccgcgctgg aacaaccatg agatgaacaa
     5401 cccgaagaag aataaataaa cgatcgggta aatgatatcc acggtcaatt ccacattgcg
     5461 gtagaacgga cggccaaatt cgccatagcg ttcgatcatg gcaaatatct tttcgggggt
     5521 cgcgaacagc atcaaatcaa gcggttggac tccgcctcgg tcatcctgca tcaatccttg
     5581 aatgaggggc agcagaaaac ccgcaaagaa cccatccaaa atgaatagca cgaaaatgag
     5641 ccaacctttc gcccatttgt aaaatcgatc tgacaaattt cctagcatgg ttcctccgtg
     5701 aataaagttt aacaaaaaaa cacgccgccg ttgctgtggt gacgtgtttt gggtcatact
     5761 taagtcctta cttcatcaag gctgcgccgc cgagcagggc atcttccacg cgttgtctgt
     5821 gttgaccatg catcggcagg ccgtccattt cctccaaaga ataatagccg aaggcggtgg
     5881 tttcattgct caaccccaat tcaccttcga tgatctctgc ttcgaaactg aggacgacaa
     5941 aaaaggcctt attcccatcg gggtacacga ccaattgatc tggattgctg tacacaccga
     6001 tcaacctcgt tgcacggacc ttcaagcctg tttcctccca aacctcgcgc tcgcaggctt
     6061 ctgctgcgct ctctccagct tccagggcgc cgccaggtac acaccaacgg ccattgtcgg
     6121 tgcgctgggt aagcaatgcc tttttacggt ctttatcgaa aatgaccgca ctacatccaa
     6181 cccgtgtttg accttccctg ccgagccgtg gaccgtacag aacctgtgtc atgttaattc
     6241 accttgtgta gattggattt tgaactcaac ggattacagg tgtaaatttt agccgagtaa
     6301 tttgtgcggt gcaatctggc ttaaagccac gaagctaaat tttgctataa aaaatctctg
     6361 agggtttctc agagattttt tctttatttg ataaatgttc cgttgcgtag ttcttgaatg
     6421 gtctgttgaa tttcttcgac cgtgttcatc acgaatggcc cgtaggggac aatgggttcc
     6481 ttgaacggcg cacctgcgat gagcatgaat tgtacgccgc tgcttgccgc gtcgcttttc
     6541 acttcgattt gttcgccgtc atcgttgaag acaaccatgc tgaccgattc aacgacctcg
     6601 cctgcgaatt cgccaacacc ttcgaacacg tatgccaaca ccgtatgtcc gctggggatg
     6661 gggtatgtga acgtcgcccc gggggccagt tttacatcca tataaagagg tgaagcggcg
     6721 atttcagtga cagggccgcg tacgccattc acttctccag cgacgagccg aattgttgct
     6781 ccatcctttt caacgacagg gatggtggac gagttcactt cctgataccg cggctgtccc
     6841 attttttggg ctgcaggcag gtttacccaa agttgaaaac cgtatatgtt gccgctctga
     6901 ccgcgcttgg gcatttcttc gtgcaaaatc ccgcgcccac tcgtcatcca ctgcacatca
     6961 cctgcgccga tggtgcctgc gttaccgagg ctgtcacggt ggttcaccga gccatccaac
     7021 atgtatgtga cggtttcaat gccgcgatgc gggtgcatgg gaaaaccgcg aatgggtcct
     7081 tcgagtgggt tgttgaaggc aaaatggtca aaaagtaaga acgggtcata ttcattgctt
     7141 gcccgcgggg caacggagcg gcgcaataat acacccgcac cctccacgac aagttgtggt
     7201 tcaataatgt gggaaatggt tctgttggtc atgtgggttg gacgatgcaa agcctgaaag
     7261 tattacgagc ggtaggccgc aatcgctgca tccactttgg aggcatccac gccgccatct
     7321 acaaggaaat cactgaagag attgaagtca tttttcaaaa tgcaaatggc gtaggcgcgt
     7381 gtttcagcag tgggggagcc gagtgcatct tccataaact cttttactgt gccgccagtt
     7441 tggtccatca gcagggctgc gtaggcatct ttgacgtccc acggcaggtc acggttccag
     7501 gtttgcaaaa gggtgggtac atgtgcgggt ttaatttcat cacgcagacc ttcgatgatc
     7561 tcaaaatcca ttgcgcggat ggccgcaagc gccttttcga taaatgggtc gttactcatt
     7621 tttagctcct tttttgagta gaactcttgc ataagtccat ttttttacca ccaaggcaca
     7681 aagtcccgaa gactcaaaga aaaaagctta gtgacttagt gcctttgtga ctttgtggtc
     7741 tacaacactt tacttatgca agaggtca
//
LOCUS       DAOQJC010000160         7711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_5163_length_7711_cov_10.755094, whole genome shotgun sequence.
ACCESSION   DAOQJC010000160 DAOQJC010000000
VERSION     DAOQJC010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5163_length_7711_cov_10.755094"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..585)
                     /locus_tag="PLE39_17195"
     CDS             complement(10..585)
                     /locus_tag="PLE39_17195"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE70122.1"
                     /translation="MNFFRRISFNFWYLRRPPWDSGITPPELFEFIQSHPAGRALDIG
                     CGTGTNVITLAKTGWQVQGFDFASRAIRIAKRKIKTAGIQADLFTDDATQMKNISGQF
                     DLALDIGCFHGIPNKADYLTQLDRILAPNGFWLMYGFFRQTDDHTAPGLVPTDLNLIS
                     AQTFTQISRRDGTDKRGRKSAWFLYQKVGGK"
     gene            complement(582..779)
                     /locus_tag="PLE39_17200"
     CDS             complement(582..779)
                     /locus_tag="PLE39_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNE70123.1"
                     /translation="MNGEVSIGKRIHFIRLNIQETAGMELAPLYNFEFTPTFIFFDAQ
                     GNEVWRQVGEFDPQLVRDSLK"
     gene            complement(828..1082)
                     /locus_tag="PLE39_17205"
     CDS             complement(828..1082)
                     /locus_tag="PLE39_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70124.1"
                     /translation="MLSLFREYSFLALAIGLVTIAGLILLTNKPRWNDYLAFVVVVAS
                     LVAAWVVLHPRQTELMGDAVTVKELIGAGTPVLLEFQSPF"
     gene            1318..3399
                     /gene="fusA"
                     /locus_tag="PLE39_17210"
     CDS             1318..3399
                     /gene="fusA"
                     /locus_tag="PLE39_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNE70125.1"
                     /translation="MKEYTTEFLRNIALVSHGGAGKTMLAEAFLHATKATTRMGKVED
                     GTTVSDYDDEEHRRKISIYSSIIPIEHRDHKINVIDAPGYTDFVGEIISALSVADGAV
                     ILVDAVAGIEVGTEIAWRYADEFNLPRFFVINKMDRDNANFEATYDAIEEFVKAHGKR
                     AIKVHLPIGEKQNFKGVVDIIGMKSYMGDGNTTADIPADLKEAADAAHFAMVEAAAEG
                     EDELMEKYLENGSLTDAEMVRGLEDVVYAGSFVPVFCAAGGHEVGAIALLNDIIDLMP
                     SPLNAPKRVAQGKGGDEELKPSDSGPLAAYVWKTTADPFVGKMTYFRVFSGSIQSDAH
                     VWNQNKGADERMAGLHLQRGKEGIPVKVVHAGDIASVSKLTATATGDTFCDKNHPLTV
                     PQPKYPAALYRVAVAPKTQADAAKISPTLTRLCEEDMTLTWENDMITHETVLKGMGDQ
                     QIDVALHRAQSKFQVGLLVHEPKIPYREGITRKAAAQYRHKKQSGGAGQFGEVHLRIE
                     PLPTADFEFTDELVGMNLSKSYLAPIEKGIKATMERGAFAGYPMSNVKIIVYDGKEHE
                     VDSKPVAFEIAGREAFKLAVQDAGPVLFEPIMNVRVIVPDAYMGDVMSDLNTRRGRVQ
                     GTETERGTTTVIAHVPMAEMMRYTTQIRSITGGRGYFTMEFDHYDTVPSHVAGPIIEA
                     HKKEMEAKKDE"
     gene            complement(3493..4332)
                     /locus_tag="PLE39_17215"
     CDS             complement(3493..4332)
                     /locus_tag="PLE39_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70126.1"
                     /translation="MPQYKVVAGTFHVKGFQPDGDSIRFQANKPEHWDFFEWETEADK
                     AAKKKQLRVESIDALETHYEGYHQPRPFAIAALESLLELLNIKSVTYSLSVTQIVDAD
                     DGKAGFIASASTDRFGRPVSYLFPKNAKLTDGAVMDSENLPIEDSINFQLAREGLVYP
                     TFYTTTDRIFAEKIRAVVARARKTKRGIWSIDKTSDFTLLDIRALQEDILLMPKLFRR
                     LVGFFDNYSDFGKLEEYMAKQRDNLVLWDGTKKKSLADLMKITGRRLQMKTPVEDILF
                     SPK"
     gene            4410..4682
                     /locus_tag="PLE39_17220"
     CDS             4410..4682
                     /locus_tag="PLE39_17220"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNE70127.1"
                     /translation="MPTVRFPALMKFYVDNQTEFEVQGATVAEVLENILVRYPALKPH
                     LYDAKGELRRHFNIFVNGVHLRELNGLATELQAGDKIILMASAAGG"
     gene            4995..5957
                     /locus_tag="PLE39_17225"
     CDS             4995..5957
                     /locus_tag="PLE39_17225"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNE70128.1"
                     /translation="MALLDIRLTQSNTIPLDSLAYHVGNTPLLPLRRVTAKLSPRVKV
                     FAKAEWFNPGGSVKDRPALNIIQNAIINGNLSGGKRLLDSTSGNMGISYATFGAALGI
                     PVTLALPASASPERISILKALGAELVLTDPLEGSDGAILKAREMVAENPDLYWYANQY
                     SNEANWQAHYLSTGPEIIAQTNGLVTHFVTGLGTSGTLMGTGRYLREQLPDVKILAFQ
                     PDAPFHGLEGLKHMPSAIKPGIYDESVAGNPLEVQTEAAHEMVSRLAREEGLFVGISS
                     GAAAVAALQVASELDEGVVVTLFPDAGYKYLSDKSLWEGKAGGR"
     gene            6001..6414
                     /locus_tag="PLE39_17230"
     CDS             6001..6414
                     /locus_tag="PLE39_17230"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNE70129.1"
                     /translation="MNLKLTKNILDDISKHVEAAYPEEGAGFLLGVEGEVKQILSLPN
                     AREDEARHNRFLFTPEDYLKAELKADSLGLSLIGVFHSHPDCPNIPSEYDREWAQPFF
                     SYIITRVDEGRSVSHRSWRLVEDRSKYEEEEISIK"
     gene            6432..6926
                     /locus_tag="PLE39_17235"
     CDS             6432..6926
                     /locus_tag="PLE39_17235"
                     /inference="COORDINATES: protein motif:HMM:NF025698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HNE70130.1"
                     /translation="MTSQTLQKVDHSALKANQYFIISLGVLAFVFNQPILAAFIALMM
                     GLGTILKKPGFGFIYTSLLKPRGWMKPDVLDDNPEPHRFAQFVGFLFMAGGSAALFLG
                     STVLGWTLVWIVVALASLNAFGGFCVGCAMYYWLVRLNVPGFAKQPPAGTFPGMKPKA
                     GATA"
     gene            6923..7330
                     /locus_tag="PLE39_17240"
     CDS             6923..7330
                     /locus_tag="PLE39_17240"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNE70131.1"
                     /translation="MSSDILLRFAFAIGIIFLGVGVNWLFNKRLISRARNGIGSLFNT
                     PPNKPTIVYFTTPDCAPCKTVQRPALEKLTSLFGEKLHVVEIDATQQPEVAKTWGVMS
                     VPTTFLLDSRGEARYVNNGVARVEKLMEQIQTL"
     gene            7449..>7711
                     /locus_tag="PLE39_17245"
     CDS             7449..>7711
                     /locus_tag="PLE39_17245"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNE70132.1"
                     /translation="MPVLRIPTPLRAYTSGQAEVTVNGSNISEALTDLTNQFPTIKPH
                     LFNDGGELRPFVNLFIGEHNIKDLQGVNTPIKDGDKVMLIPSIA"
BASE COUNT         1837 a         1988 c         2026 g         1860 t
ORIGIN      
        1 aattaccaat tacttccccc ctactttctg atacaaaaac catgccgatt ttcgtccgcg
       61 tttgtcggtg ccatcgcggc gggagatctg ggtaaatgtt tgggctgaga tcaggttcag
      121 gtcggtgggg acaagcccgg gcgcggtgtg atcgtcggtc tggcggaaga atccgtacat
      181 gagccagaat ccgttggggg cgaggattcg gtccagttgg gtcagataat ccgctttgtt
      241 ggggatgccg tggaaacaac cgatgtcgag cgcaaggtca aattgcccgc tgatgttttt
      301 catttgggtc gcgtcgtcgg taaagaggtc ggcttgtatg cctgcggttt ttatttttcg
      361 cttcgcaatg cggatggcac gtgaggcaaa gtcgaagcct tgcacctgcc agcctgtttt
      421 ggcgagtgtg atgacgttgg tgcccgtgcc gcagccgata tcaagcgcac gccctgctgg
      481 gtggctttgg atgaattcga agagttcggg cggggtgatg ccgctatccc agggcgggcg
      541 gcgtaaatac caaaagttga aagagatgcg gcgaaagaaa ttcatttgag cgagtcgcgc
      601 accagttgcg ggtcaaattc accgacctgc cgccagactt cgttgccctg tgcatcgaag
      661 aagatgaagg tgggggtgaa ttcaaaattg tagagcgggg caagttccat gcctgcggtt
      721 tcctgaatgt tcaagcggat gaagtgaatg cgtttgccga tgctgacttc accgttcaat
      781 tcctgttcga gctcgtcaac gacgggcttt aattgggtgc aggtaattca gaagggagat
      841 tggaactcaa gcaaaacggg tgtgcctgcg ccgatgagtt ccttcaccgt cacggcatca
      901 cccatcaact cggtctggcg cggatgcaga acgacccagg ccgcaacaag gctggcaacc
      961 accactacga acgccagata gtcgttccat cttggtttgt tggtcagcag gatcaacccc
     1021 gcaatggtca ccagcccgat ggccagcgcc agaaaggagt attcacgaaa caatgagagc
     1081 aagttttcct ccgttttagg tggagtggaa ggtccatatt caagaaatca ccccgaaggg
     1141 tgaattttgc ggattaaatg tcatgcgcag gccgtgacgg ttcacacttt gacaattcaa
     1201 ttgagttctt ttacaatcgt gcaacttgtc caaaggttta ccgggattcc tctggcttcg
     1261 ttctggacag gtgaaaagaa ctgctttaca tcctacaaat ataggaaggc tactttcatg
     1321 aaagagtata ccaccgagtt tcttcgcaac atcgcgctgg tttcacatgg aggcgcgggg
     1381 aaaacgatgt tggcggaggc atttttacac gcaacgaagg caacaacacg tatggggaag
     1441 gtcgaagacg gaaccacggt ttcggattac gacgacgagg aacaccgccg aaagatctcg
     1501 atttattcaa gtatcattcc cattgagcat cgcgatcata agatcaatgt aattgacgcg
     1561 ccaggctaca ccgattttgt gggtgagatc atctccgccc tgagtgtggc tgatggcgcg
     1621 gttattttag tggatgccgt cgcgggcatt gaagttggta cggaaatcgc atggcgctac
     1681 gcagatgaat tcaatctgcc gcgctttttt gttattaata agatggatcg tgacaatgcg
     1741 aacttcgaag ccacctatga tgccattgaa gaatttgtaa aagcacacgg caaacgtgcc
     1801 atcaaggtcc atttgccgat cggtgaaaag cagaatttca agggcgtggt ggatatcatc
     1861 ggcatgaaat cctacatggg tgatggaaac accaccgcag atatcccagc cgatttgaaa
     1921 gaagcagcag atgccgccca ctttgccatg gtggaagccg ccgccgaagg cgaagacgaa
     1981 ctgatggaga aatacctcga aaacggttct ttgaccgacg cggaaatggt acgcggcctc
     2041 gaagatgtgg tctacgctgg ttcattcgtc cccgtattct gtgcggcagg cggacatgaa
     2101 gtcggtgcca ttgccctgct caacgacatc attgacctga tgccctcccc attgaacgct
     2161 ccgaagcgtg tggctcaggg caagggcggt gatgaggaat tgaaaccgtc tgattctggc
     2221 ccattggccg cctatgtttg gaaaaccaca gccgacccat ttgtcggcaa gatgacctac
     2281 ttccgcgtgt tctcgggttc gatccagtca gatgcgcatg tttggaatca aaataaaggc
     2341 gccgatgagc gcatggctgg tctgcatctc cagcgcggca aggaaggcat tcctgtgaag
     2401 gtcgttcatg cgggtgatat cgcatctgta tcgaagttga ccgccactgc cacaggcgat
     2461 accttctgcg ataagaatca ccctctcacc gttccccagc cgaaatatcc cgctgccctg
     2521 tatcgcgtgg cggttgcacc caagacccag gccgatgcgg ccaagatctc tcccaccctc
     2581 acccgcctct gcgaagagga tatgaccctc acgtgggaaa atgacatgat cacccatgaa
     2641 accgtattaa aaggcatggg cgaccagcaa attgacgtgg ctttgcatcg cgcacagtcc
     2701 aagttccagg tgggtctgct tgtccatgag ccaaagattc cctaccgtga gggcattacc
     2761 cgcaaggcag cggcccagta tcgccacaag aagcaatcag gcggcgcggg gcaattcggc
     2821 gaagtgcatc ttcggattga acccctgcca accgctgact ttgaattcac cgatgaactc
     2881 gtcggcatga acctctccaa gtcctatttg gcgcccattg aaaagggcat caaagccact
     2941 atggaacgtg gcgcttttgc aggctaccca atgagcaacg tcaagatcat tgtgtacgat
     3001 ggcaaggaac atgaagtcga ttcaaaacca gtcgcctttg aaattgctgg ccgtgaagcc
     3061 ttcaaacttg ccgtacagga tgccggcccg gtcctgtttg agcccattat gaatgtgcgt
     3121 gtgatcgttc ctgatgccta catgggcgat gttatgagcg atctaaacac ccgtcgcggc
     3181 cgtgtacagg gtaccgaaac cgagcgcggc accaccacgg tcattgccca cgtgccgatg
     3241 gctgaaatga tgcgctacac cacacagatc cgctccatca ccggcggacg cggctacttc
     3301 accatggaat tcgaccacta tgacaccgtg ccctcccacg tggcagggcc gatcatcgaa
     3361 gctcacaaga aggaaatgga agccaagaag gacgagtagt tcagtaattg agtacgctca
     3421 gtcaataata aaacagtccc gaagccaggt gcctcgggac tgttttatct tccaccgatt
     3481 actgccttcc ccttacttcg gcgaaaacag aatatcttcc actggggttt tcatttgcag
     3541 gcgtctgcct gtgatcttca tcaaatctgc cagtgacttt ttctttgtgc catcccacaa
     3601 aaccaggtta tctctttgtt tggccatgta ttcttccaac ttcccaaaat cggaataatt
     3661 atcgaagaag cccaccaaac ggcggaagag ttttggcatc agcaaaatat cctcctgcaa
     3721 agcgcggata tccagcaacg tgaaatcaga tgtcttatct atggaccaga tgccgcgctt
     3781 ggtcttacga gcacgcgcca ccaccgcacg gatcttttcg gcaaagattc tgtccgtggt
     3841 cgtgtaaaac gttgggtaaa ccaacccttc acgcgcaagt tgaaaattga tcgaatcctc
     3901 aatgggcagg ttttctgaat ccatcacggc gccgtcggtg agttttgcat tttttggaaa
     3961 gagataactc acggggcgtc cgaagcggtc tgtcgaagcc gaggcgatga aacccgcctt
     4021 gccgtcgtct gcatccacga tctgggtcac gctcaaactg tatgtcaccg atttgatatt
     4081 gagcaattcc aacagagatt ccagggccgc aatcgcaaag ggacgcggct ggtgatagcc
     4141 ttcgtaatgg gtttcaagtg catcgatcga ttccacgcgc agttgctttt tctttgccgc
     4201 cttatcggct tctgtttccc attcaaaaaa atcccaatgt tcgggtttgt tcgcctgaaa
     4261 tcggatcgaa tcgccgtcgg gttgaaaccc cttcacatga aacgtacctg caactacttt
     4321 gtattgtggc atggtcatct ccttggttga tggcctcatt atagggaatt tccccgccga
     4381 aggcaatcaa ctcacagtaa aataaataca tgcctactgt ccgctttccc gccctgatga
     4441 aattttatgt cgataaccaa accgagttcg aagtgcaagg cgccaccgtt gccgaagtgc
     4501 ttgaaaacat cctcgtgcgt taccccgctt tgaaaccgca cttgtatgat gcaaaaggcg
     4561 aactgcgcag gcacttcaac attttcgtca atggggtgca tttgcgtgaa ttgaatggat
     4621 tggcgacgga attgcaagca ggcgacaaga tcattctgat ggcctccgcc gctggaggat
     4681 gaacgcttca cgcccaacgg aagcctgatt cggttttctg aaaaagcgac ctccactcgg
     4741 ggaattttag tattgacaaa ttgggtaaga tacgtaaaat aacgcgcatc taactggaaa
     4801 cctatggata cattatttaa ctattgcttg tgtctatcgc ggggctcgta accacccgcc
     4861 tgagatagac ccctccgcag tcaacggaaa cagacggttg gacgaccccc gcttgcagaa
     4921 ttttttgcat agtgtggcgc tcgttcgccg tctttttgtt tacccgttga ctgcttttga
     4981 tttaaaagga tgacatggcc ttactggata ttcgacttac ccaatccaat accattcccc
     5041 tcgatagcct tgcctatcat gtgggaaata cccccctgct cccactgcgg cgggtgaccg
     5101 caaaactctc tccacgtgtg aaggtgtttg ccaaagccga gtggttcaac cccggcggat
     5161 cggtcaaaga ccgccctgct ttgaacatca tccagaatgc catcatcaac gggaatttga
     5221 gcggtggaaa gcgcctgctc gattccacct caggcaatat ggggatttcc tacgccacct
     5281 ttggcgcagc acttggaatt cctgtcaccc ttgccctgcc agccagcgcc agccccgagc
     5341 gaatttccat cctcaaagcc ctcggcgcgg aattggtact caccgacccg ctcgaaggct
     5401 cagacggtgc cattctcaaa gcgcgtgaaa tggttgcgga aaacccagac ctgtattggt
     5461 acgccaatca gtacagcaac gaagccaatt ggcaggccca ttacctctct accgggcctg
     5521 agatcatcgc ccaaaccaac gggctggtga ctcatttcgt aacgggtctc ggcacctctg
     5581 gcacgttgat gggcaccgga cgatatttgc gcgaacaact gccagatgtg aagattttgg
     5641 ctttccagcc tgatgcaccc ttccacgggc tggaagggtt gaagcacatg cctagtgcga
     5701 tcaaacccgg tatctatgac gagtcagttg cgggcaaccc gcttgaggtc cagaccgaag
     5761 cggcgcacga gatggtatca aggcttgcac gggaggaggg tctctttgtg gggatctctt
     5821 ccggggcggc ggctgtcgct gcgcttcaag ttgcaagtga attggatgaa ggtgtggttg
     5881 taaccttgtt ccccgatgcg ggctataaat atttgagcga taaatctttg tgggagggaa
     5941 aggctggtgg acggtaggcc gcaggttgtg tctatcgtcg gcagttgttt caggtgaaca
     6001 atgaatttga aacttacgaa aaatattctt gatgatatca gcaaacatgt ggaggccgcc
     6061 taccctgaag agggggctgg ttttttgctc ggcgtggaag gtgaagtgaa gcagatttta
     6121 tccctgccca atgcgcgcga ggatgaagca cgccacaatc ggtttttgtt cacccccgaa
     6181 gattacctga aagccgagtt gaaggccgat agcctcgggt tgagcctgat cggtgttttt
     6241 cattcacacc ccgattgccc gaacattccc tccgagtatg accgcgaatg ggcgcagccg
     6301 ttcttctcct acatcatcac acgggtggat gaaggcaggt cggtcagtca tcgttcatgg
     6361 cggttggtcg aagatcgttc gaagtatgaa gaagaagaga tttcaataaa ataaaaacag
     6421 gataccttaa catgacatca caaaccttac aaaaagtgga tcattctgca ttaaaggcca
     6481 accaatattt catcatttcg ttgggcgtgc ttgcttttgt tttcaaccag ccgatccttg
     6541 cggcattcat cgcgttgatg atgggacttg gcaccatttt gaagaaaccc ggcttcggat
     6601 ttatctacac atccctgctc aaaccacgcg gatggatgaa gcccgatgtg ttggacgata
     6661 accccgaacc gcatcggttt gcgcaattcg tcggattttt gttcatggct ggcggatcgg
     6721 ccgctttgtt cctcggttca acggtcctcg gctggaccct ggtgtggatc gtcgttgccc
     6781 tggcctcgct caacgccttt ggcggttttt gcgtgggatg cgccatgtat tactggctgg
     6841 tgcgcctgaa cgtgcctggc tttgccaaac agccgcccgc ggggaccttc cccggcatga
     6901 agccgaaagc aggagccaca gcatgagttc agatatcctg ttgcgttttg cttttgcaat
     6961 tggtattata tttttaggcg tgggcgtcaa ctggctgttc aataagcggt tgatctcacg
     7021 ggcccggaat ggcatcgggt ccctgttcaa tacgccgccg aacaagccca ccattgtcta
     7081 tttcaccacg ccagattgtg cgccctgcaa aaccgtgcag cgccccgccc tcgaaaagct
     7141 gaccagcctg ttcggtgaaa aactgcacgt cgttgaaatt gacgcaaccc agcagcccga
     7201 ggttgcaaag acctggggtg tcatgagtgt gccgaccaca ttcctgctcg attcccgtgg
     7261 tgaggcacgt tatgtcaata acggtgtggc gcgggttgaa aagttgatgg aacagataca
     7321 gactttgtaa atcatttacc acgaaggtca caaagtgtca caaaggaaaa ccttaagtcc
     7381 gtgtttcctt cgtgtacttc gtgccatttg tgtttaagtt ttttcaaaat tattttagga
     7441 gactctacat gcccgttttg cgaatcccga ccccgttgcg tgcttatacc agcggacaag
     7501 ccgaagtgac agtcaatggc tcaaatattt ccgaagccct gaccgatctg accaatcaat
     7561 ttccgacgat caagccgcat ttgttcaacg atggcgggga actgcgtccg tttgtcaatt
     7621 tgttcattgg cgaacataac atcaaggatt tgcagggcgt gaatacgccg atcaaggatg
     7681 gcgataaggt aatgttgatt ccgtccattg c
//
LOCUS       DAOQJC010000161         7657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_5216_length_7657_cov_7.840042, whole genome shotgun sequence.
ACCESSION   DAOQJC010000161 DAOQJC010000000
VERSION     DAOQJC010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7657
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5216_length_7657_cov_7.840042"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..987)
                     /locus_tag="PLE39_17250"
     CDS             complement(94..987)
                     /locus_tag="PLE39_17250"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HNE70133.1"
                     /translation="MTEQTFNWQAWGLAIRPRTLPAAAAGVVMGGALAWHDGFFRLDA
                     TLVCLFAALLLQIGSNLANDVFDFERGTDTPERLGPTRVTQAGLLTSSQVKLGMAVVF
                     VLSALMGLYLIWLGGWVILAIGIAAIISAIAYTGGPFPLGYYGLGDVFVFIFFGLASV
                     AGTYYVQAGDVSSAAWWMTLPPGLIITAILVVNNLRDLENDRKAGKYTLAVMFGEQFA
                     KAEYLTCIFAAYLILPVAAWFGLIPWLSLMAWASFPIAFRAIRTVFTQKGRPLNAALA
                     GTGQAAFMFSLLFWIGLALQR"
     gene            1058..1750
                     /locus_tag="PLE39_17255"
     CDS             1058..1750
                     /locus_tag="PLE39_17255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HNE70134.1"
                     /translation="MTQLTGRERAAYVQNMFTQIAKRYDLMNRLMTGGQDIRWRKMVI
                     KLTRMTNTASLLDLGTGTGDLAREALTQFPQARITASDFTLEMMRVGKKNGPLNFSTA
                     DALHLPFNSTTFDAVISGFLMRNVINLDKAIEEQYRVLKNGGRIVILDTTRPKKNILS
                     PFIWIHMHIIIPTLGGLLTGVREAYRYLPETTEGFVTAEDMASRMSKAGFQNVNFKRL
                     MFGTIAIHWAEK"
     gene            complement(1817..3049)
                     /locus_tag="PLE39_17260"
     CDS             complement(1817..3049)
                     /locus_tag="PLE39_17260"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE70135.1"
                     /translation="MFNKLSLTFHRFPRTFWVVAGTHFIDVIGNTLLMPFFALYVTQK
                     FGVGMTEAGIILATNSVAGLIGSTIGGAMADRFGRRGIILFGLIVSAFSGLTLGFVNQ
                     FYMFYGLSVFVGLMGSAAHPAHNAMIADILPAEQRAEGFGIMRVISNFAWIIGPTIGG
                     FLAAYNFLYLFLSDAVISSITAVLVFRLIPETKPQPKEKTEHETLAQTIGGYRVALFD
                     RPFLAFIFASILMILVYQQMYSSLSVYLRDVHNVNPRFYGLVMSSSAVTVVIFQFWVT
                     SIIKKYPPFLMMATATFFYMIGFGMYGFVSTIPLFILAMVIITIGEMVGMPTSNVLAA
                     SFAPEEMRARYMAVFGLTWMVPSMIGPWAAGLIFDNMNPNYVWYISIVLCLIAMLAFY
                     LLQVRLGGREQFQRAKDG"
     gene            complement(3110..5518)
                     /locus_tag="PLE39_17265"
     CDS             complement(3110..5518)
                     /locus_tag="PLE39_17265"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE70136.1"
                     /translation="MLRARWYKVINDLFGNKTRTLLIVLSMSVGLFALGIILSARTIL
                     ADGLAESFATIYPSSGTVRTIELFGEDFIESVRKMDDVQEADARRSISVRMQTKSGEW
                     KNLTIFVFADYDDIRVNKVTSQSGAWPPPEREILIERAALSVIEAQVGDVILIRFPND
                     VERRIRIAGLAYDPAQLPAQIDGTPYGYITFDTLEWFGESYGFNELHVIAVNPEDKDW
                     AQQVVNRVKDKAENSGYTIPLSMTAEPGQLPMNDVLQGILLLMGLLGVLSLFLSIFLV
                     VNTVSALLTQQKRQIGVMKAVGGGTLQILGMYLVMVFSYGVLALLIAIPFGMYGAREL
                     SSVLAVFFNFDLYSMDVPPQTYLIQAAVGLILPVLASLVPFLSNLRISAAEAMSSYTM
                     GRGRFGKNWIDQLLSGANLWFARMLSIRSILLSVRNTFRNKGRLTLTLITLTLGSATF
                     ISVFNVRASLSSTVEDMIKWFNCDTMFALDRSYRADKVQREAESIPGVTQTDVWIQLP
                     TRMIRRDGSESGMIYMFAPTVRPDSLIVSPSLAEGRWLAPGDENAVVVPSALLNDEPE
                     LELGGDIVLKIDGDEHVFKIVGTFKGMAFLPIMYVNYDYITKITNRIGQADAVMVATQ
                     QHDAAYVDAVTRTLEERFNRVGVEIGLATTINTERRDAEASFDSIVALLLLMAILLAL
                     VGGLGLMGTMSINVLERTREIGVLRAIGAPNRSVASVFILEGIIIGLMSWAMGAAVAI
                     PMSRGLNQALGQAVMGVPLTYSYSLPGLWLWLVIVILLSVLASFIPARTASRLTVREV
                     LAYE"
     gene            complement(5531..6217)
                     /locus_tag="PLE39_17270"
     CDS             complement(5531..6217)
                     /locus_tag="PLE39_17270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE70137.1"
                     /translation="MNSSTDKPLIELRSVVKVYESLAGSVTALNGIDLQVFQGEFLVV
                     TGKSGSGKTTLVNMLTGLDRSTSGEIWVNGEPLHKFGPEKAARLRGRTMGVIFQTFEL
                     LPTLTVLQNVMLPMDFARRYSRWEQRKRAYHLLEQVGIADHARKLPTALSGGQQQRLA
                     IARAMANDPAILVADEPTGSLDSATANAVVDIFAGLVQEGGTVFLVTHDKDIAKFGSR
                     VITLSDGEIV"
     gene            complement(6198..6935)
                     /locus_tag="PLE39_17275"
     CDS             complement(6198..6935)
                     /locus_tag="PLE39_17275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE70138.1"
                     /translation="MLEVDLHPKNTPVNPPDSDLVLLREVVKTYHGSAGTFTALKGVD
                     LCVESGAFVSIIGKSGSGKSTLINVITGIDRPSSGEVIVDRTPVHTLNEEQIAIWRGR
                     SVGVIFQFFQLLPTLTAVENILLAMDYGKHYPRLERPERAMQLLELVGMADYAHRLPG
                     SLSGGQQQCVAIARALANDPVLLTADEPTGNLDSKSTEMVFQLFENLAGTGKAILMVT
                     HDNELAVRAQRTIRLVDGRIQDEFVNR"
     gene            complement(7200..>7657)
                     /locus_tag="PLE39_17280"
     CDS             complement(7200..>7657)
                     /locus_tag="PLE39_17280"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE70139.1"
                     /translation="LRVLYHSPNAQPAYGAKPVDLDTLLRESDFVSINVPLKAETRHL
                     VNADFLAKMKPNAILVNTARGGVLDQTALYDALKSKRIFAAALDVTDPEPLPMDCPLL
                     ELENCIIVPHLGSASQKTRDMMSLLAAQNLIAGLKGERLTHCVNPQVYG"
BASE COUNT         2062 a         2109 c         1871 g         1615 t
ORIGIN      
        1 cagttgattg gctggaaaga aaatggtgta aatggaagtt tgtgagttga aggtttgaaa
       61 aatcaatctt ctttcttctt acttctttct tttctacctc tgcagcgcca atccaatcca
      121 aaacaacaaa ctaaacataa atgccgcctg ccctgtaccc gcaagcgcag cgttaagcgg
      181 acgtcctttt tgggtaaaga ccgtgcgaat ggcgcggaag gcaattggaa acgaagccca
      241 tgccattaaa gacaaccacg gaatcaaccc aaaccacgcc gcaacaggca aaatcaaata
      301 agcggcaaaa atacaggtca aatattcagc ctttgcgaat tgttcgccga acatcaccgc
      361 cagcgtatac ttgccagcct tgcggtcatt ttcaaggtcg cgcagattat tgaccaccaa
      421 aatcgcagtg atgatcagcc ctggtggaag tgtcatccac cacgcagcag aagaaacatc
      481 gccagcctgc acgtagtaag tccctgccac cgaagcaaga ccaaaaaaga taaagacaaa
      541 aacgtcgccc aacccgtagt aaccaagcgg aaacggcccg cctgtgtaag cgatggcgga
      601 aatgatcgca gcaataccaa tggcaaggat gacccaaccg ccaagccaga taaggtaaag
      661 ccccatcaac gccgaaagta caaagacaac cgccattccc aacttcacct gtgacgatgt
      721 cagcaagcca gcttgagtca cacgcgtggg gccaagccgt tcaggggtat ctgtaccgcg
      781 ctcaaaatca aaaacatcgt ttgcaaggtt gctgccgatc tgtaaaagca gggcggcgaa
      841 taaacaaacc agcgtggcat caagccgaaa aaatccgtca tgccacgcaa gcgcgccgcc
      901 catcaccaca ccagccgccg cagcaggcag tgtgcgtgga cggatcgcca atccccaggc
      961 ctgccaatta aatgtttgct cggtcaaagt ttgctccagt aagtttgatg gcgggcagta
     1021 taacttataa ttgcgcaaat tattttcagg agatttcatg acccaactta caggacgcga
     1081 acgcgccgcc tacgtccaaa acatgttcac gcagatcgcc aaacgctacg atctgatgaa
     1141 tcgactgatg actggcgggc aggatatccg ctggcgcaag atggtcatca aacttacacg
     1201 gatgacgaac accgcttcct tgctggacct gggaactggt acaggcgatc tggcacgcga
     1261 agccctgacg cagtttccac aagcccgcat caccgcttca gatttcaccc tcgaaatgat
     1321 gcgcgtcggc aaaaagaacg gccctctgaa tttttccaca gccgacgcgc tgcacctgcc
     1381 cttcaacagc accacgttcg atgcggtcat ttcgggcttt ctcatgcgca acgtcatcaa
     1441 tctggataaa gccatcgagg aacaatatcg cgtgctgaaa aatggcggac gaatcgtcat
     1501 tctagacacc acccgcccga agaaaaatat cctctcccca ttcatctgga tccatatgca
     1561 catcatcatc cccaccctgg gtgggttact gacaggggtt agggaagcgt accgctatct
     1621 gcctgaaacc acagaaggtt ttgtcactgc cgaagacatg gcgtcgagaa tgtcaaaagc
     1681 ggggttccaa aatgtcaatt tcaaaagatt gatgttcgga acgatcgcaa tccactgggc
     1741 agaaaaataa aaaaggtgga aagtagaaag cggaagacac aatcccgcat ttcactttcc
     1801 acctttcatt ttctacttac ccgtcttttg cgcgttggaa ctgttcccgt ccacccagcc
     1861 gcacttgtaa cagataaaac gccagcatgg cgatcaaaca cagcacaata ctgatgtacc
     1921 agacatagtt tggattcata ttgtcgaaga tcagccctgc cgcccagggg ccgatcatcg
     1981 aaggcaccat ccaggtcagg ccaaagaccg ccatgtaacg ggcgcgcatc tcctcgggcg
     2041 caaaggatgc agccagcaca ttgctggtgg gcataccgac catctctcca atggtaatga
     2101 tgaccattgc cagaataaac aacggaatgg tggaaacaaa tccgtacata ccaaagccga
     2161 tcatataaaa gaaagtagcg gttgccatca tcaaaaacgg cggatatttc ttgatgatgc
     2221 tcgtcaccca aaactggaag atcaccaccg tcacagcgct tgaagacatc accaatccat
     2281 aaaaccgcgg attgacgtta tgaacatcgc gcagatacac cgaaagcgaa ctgtacatct
     2341 gctgatacac caggatcatc aagatggatg caaagatgaa agccagaaac ggacgatcaa
     2401 agagcgccac acgatatccg ccgatggtct gcgccagcgt ttcgtgctcg gttttctctt
     2461 tgggttgagg cttggtctcg gggatcaaac ggaagaccag cactgcggta atactgctga
     2521 tgaccgcatc gctcaaaaac aggtaaagga aattataggc agccagaaat ccaccgatgg
     2581 tggggccgat gatccacgcg aagttggaga tcacgcgcat gatgccgaag ccctcagccc
     2641 tttgttcggc gggaaggata tccgcgatca tggcattgtg agcaggatgc gcagcgctgc
     2701 ccatcagccc aacaaatacc gacaagccgt aaaacatata gaactggttc acgaacccaa
     2761 gcgtgagtcc gctaaaggca ctaacgatca atccgaaaag gatgatgccc cggcgcccaa
     2821 aacggtcagc catggcaccg ccaatggtgc tgccgatcaa ccctgccacc gaattcgtag
     2881 cgaggatgat acccgcctcg gtcatgccca caccaaactt ctgggtgaca tataaagcaa
     2941 agaacggcat gagcaaagta ttgccaatca catcaataaa atgagtccct gccaccaccc
     3001 agaacgtgcg tggaaagcgg tgaaaggtaa gggataactt gttaaacatt tttctcctat
     3061 aacatatttg gacatatgac tgcctgcaaa acaacggcgg tcatgtgtgt tattcgtagg
     3121 caagcacttc gcgaaccgtt aatcgggaag ccgtacgtgc cgggatgaaa ctagccagaa
     3181 cactcaaaag gatgacgata accaaccaca accaaagccc ggggagtgaa taggaatagg
     3241 tcaatggcac gcccatcacc gcctgaccga gagcctggtt caaacccctg ctcatgggga
     3301 tggcgaccgc cgcgcccatc gcccaactca tcaaaccgat gatgattcct tcgagaataa
     3361 aaacggacgc cacactacga tttggcgccc caatggcgcg caatacaccg atctcgcggg
     3421 tgcgctccaa aacattgata ctcattgttc ccatcagccc caacccgccg accaaagcca
     3481 gcaggatcgc catcaacaac aaaagggcaa cgatcgaatc aaaagaagcc tcggcatcac
     3541 gccgctctgt gttgatggtg gtcgccagtc ctatctcaac gccgacacgg ttaaaacgtt
     3601 cttccagcgt acgagttacg gcatccacat aggcagcgtc atgttgttgt gtagcaacca
     3661 tcaccgcatc tgcctggccg atgcggttgg tgatcttcgt gatgtaatca taattgacgt
     3721 acatgatcgg aaggaacgcc atgcccttga atgttcccac gatcttgaac acgtgttcat
     3781 caccgtcaat cttcagcaca atgtcgccgc cgagttcgag ttctggttca tcgttcagaa
     3841 gcgcagaagg gacgaccact gcattctcgt cacctggagc aagccacctg ccctccgcaa
     3901 gggaaggtga aacgatcaaa ctatccgggc gaacagtggg ggcgaacatg tagatcatgc
     3961 cgctttcact tccatccctg cggatcatcc gcgttgggag ttggatccac acatcggttt
     4021 gggtcacacc gggaatgctt tccgcctcac gctggacctt atcagcacga taacttcgat
     4081 ccaaagcgaa catcgtatca cagttgaacc acttgatcat atcctcaaca gtgctggata
     4141 acgaagcacg gacgttgaac acactgataa aggttgcgga ccccagcgtg agcgtaatca
     4201 gagttaacgt caacctgccc ttgttgcgga aggtattccg caccgaaagc aagatcgagc
     4261 ggatggataa catccgagca aaccataaat ttgcaccgga aagcaattga tcaatccaat
     4321 ttttaccgaa gcgtcccctg cccatggtgt agctgctcat cgcctccgcc gcgctgatcc
     4381 gcaggttgga gaggaacggc acgagggagg caaggaccgg caggatcaat ccgacagcgg
     4441 cttggatcag atatgtttga ggcggcacat ccatcgaata aagatcgaaa ttaaagaaca
     4501 ccgccagaac agaactgagt tcccgtgcgc catacatccc aaacggaatg gcgatcagca
     4561 acgccagcac accataggaa aataccatca ccagatacat ccccagaatt tgaagcgtgc
     4621 cgccaccaac cgctttcatc acaccgatct gccgcttttg ctgggtaagc aaagcagaca
     4681 cggtgttgac cacaaggaag atgctcagaa agagagacaa cacaccaagc agccccatta
     4741 acagaaggat gccttgcaaa acatcattca tcgggagttg acctggctcc gcggtcatac
     4801 tcaacggaat ggtgtagcca gaattttcgg ccttgtcctt gacgcgattg accacctgtt
     4861 gtgcccaatc cttgtcttca gggtttactg cgatcacatg caattcgtta aacccatagg
     4921 attcgccgaa ccattccaat gtatcaaagg tgatgtaacc gtacggtgtg ccgtcgatct
     4981 gcgcagggag ttgggcaggg tcatatgcca accctgcaat gcgaatcctg cgctcgacat
     5041 cgttcggaaa cctgatcagg atcacgtcac caacctgtgc ttcgatgaca gagagcgccg
     5101 cgcgttcgat cagaatctca cgctcgggcg ggggccaggc cccgctttgt gaggtgacct
     5161 tgttcacgcg gatatcgtca taatcggcaa acacaaagat ggtgaggttt ttccactcgc
     5221 ctgattttgt ctgcatgcgg acggagatac tcctgcgtgc gtcggcttcc tgcacatcat
     5281 ccatcttgcg gacagactca atgaaatcct caccgaacag ttcgatcgtg cgcacggttc
     5341 cgctggacgg gtagatggtt gcaaaacttt ccgccaatcc atccgccaga atagtgcgtg
     5401 cgctaagaat gatccccaat gcaaaaagcc caaccgacat ggacagcacg atcaaaagcg
     5461 tgcgcgtctt gttgccaaat aaatcgttga taaccttgta ccagcgcgcc cttagcatgg
     5521 agattctcct ttaaacaatt tcaccatcag acaaggtgat cacccgtgaa ccgaatttgg
     5581 caatatcctt gtcatgcgta acgagaaaaa cggtcccgcc ttcctgaacc aaccccgcaa
     5641 agatatcaac gactgcgttc gctgttgcag agtcaaggct gccggtgggt tcatctgcaa
     5701 caaggatggc agggtcgttg gccatggcgc gggcaatcgc cagccgttgt tgctgcccgc
     5761 ctgataatgc ggtcgggagt ttccttgcat gatcggcgat ccccacctgt tcgagtaagt
     5821 ggtaagcacg cttgcgctgc tcccagcggg aatagcgcct agcgaaatcc atcggcagca
     5881 tcacgttttg caaaacggtc aaagtaggca gaagttcaaa cgtttgaaag atcacaccca
     5941 ttgtcctgcc ccgaagcctt gcggcttttt cagggccaaa cttgtgtaac ggctcaccat
     6001 tgacccagat ctcccctgaa gtggagcgat ccaaccctgt aagcatattg accagggtgg
     6061 tcttgccgct tcctgatttt ccagtgacca ccaaaaactc gccctgaaag acctgcaggt
     6121 caatgccgtt caaagcagta actgaacctg ccaggctttc atagacctta accacggagc
     6181 gcaactcgat caatggctta tcggttgacg aattcatctt gaatcctccc atccaccagg
     6241 cggatcgtgc gctgggcgcg gacggcaagc tcgttgtcgt gcgtgaccat gagaatggcc
     6301 ttgccagtgc ctgcaagatt ttcaaaaagt tgaaacacca tctccgtgga ctttgaatca
     6361 agattgcctg tcggctcatc ggcagtgagc aggacgggat cgttcgccaa tgcgcgtgcg
     6421 atcgccacac attgctgctg cccgccggaa agcgagcccg gcaggcggtg ggcataatct
     6481 gccatgccga ccaattcaag caattgcatg gcccgttccg ggcgttcgag ccgcggatag
     6541 tgcttcccat aatccatggc cagcaaaata ttttccacag ccgtcaatgt gggcagaagt
     6601 tgaaagaatt ggaagatgac accgaccgaa cgaccacgcc agatggcgat ctgctcctcg
     6661 ttcaaggtat gtaccggggt acggtcaacg atgacttcac ctgaactggg cctgtcaatg
     6721 ccagtaatga cattgatcag cgtggattta ccgctgcccg atttcccaat gatcgaaaca
     6781 aaagcgcccg actcgacgca caagtccacg cctttgagcg cggtgaatgt ccctgccgag
     6841 ccgtggtagg ttttaacgac ctcccgcaaa aggaccagat cggagtcggg agggttgact
     6901 ggggtgtttt tgggatgcag atcaacttcc agcatgatgt tttcctaacc ggttcccggt
     6961 gtaacactcg cccaattgcc acccttggcg gtattgcctg cactgggaac atttttttat
     7021 ttgacctctt gcatacgttg caaggaatat ctttgaaaag ccattttgga ccacagagtt
     7081 tttttattta ttttctctgt gtctccgtgg ctcagtggtt aatctttcag ccttttgcat
     7141 gaagtctatt aaacctctgg caggagttta gaccacatgg cttagtgttt caaaatgatc
     7201 tacccataca cctgcggatt tacacaatgg gtcagacgtt cgcccttcag cccggcgatc
     7261 aggttctgag ccgcaagaag tgacatcatg tcgcgagtct tttggctggc acttccgaga
     7321 tgcggcacga taatacagtt ctccaattcc aaaagcggac aatccatcgg cagcggttcg
     7381 gggtcggtga catcgagtgc ggcggcgaaa atgcgtttgg atttgagcgc atcgtacaag
     7441 gcggtttggt caaggacgcc cccgcgggca gtattgacca gaatcgcatt cggtttcatt
     7501 ttcgccaaaa aatctgcatt caccagatgc cgtgtctcag ctttgagcgg cacattgatc
     7561 gagacaaaat cggattcgcg caaaagggtg tccaggtcca caggttttgc gccgtaagca
     7621 ggctgtgcat ttgggctgtg ataaagaacc cgcagat
//
LOCUS       DAOQJC010000162         7518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_5377_length_7518_cov_8.762428, whole genome shotgun sequence.
ACCESSION   DAOQJC010000162 DAOQJC010000000
VERSION     DAOQJC010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5377_length_7518_cov_8.762428"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..694
                     /locus_tag="PLE39_17285"
     CDS             65..694
                     /locus_tag="PLE39_17285"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNE70140.1"
                     /translation="MKSTRDKILQTLLKKPRSTINDLAEAVGINPISVRHHLTNLQME
                     GLVEGQEERHGVGRPRLVYVLTSEGMEHFPTRYMLLTTRLLSQMKETMPGPVVAKLFN
                     QIAEDLASQYANDMKHLSMEERLDFVKDMLAQEGFTVEWEKKDGNYQIHEISCPYYQI
                     GVAHPEVCTVDQTLISKMLALPANKVQCILDGGAHCTYVVQPVVEVKSK"
     gene            704..1048
                     /locus_tag="PLE39_17290"
     CDS             704..1048
                     /locus_tag="PLE39_17290"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNE70141.1"
                     /translation="MSEETKEIQWTIHKTHPEMVKNARDRLSEVVDPEIGMNIIQLGL
                     VRDIEIENNIAHLKMILTTPFCPYGPAMIEMTKAKATEGLNMPVNIEMGMDVWDFSMM
                     EDPSALDWGMYA"
     gene            complement(1135..1746)
                     /locus_tag="PLE39_17295"
     CDS             complement(1135..1746)
                     /locus_tag="PLE39_17295"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNE70142.1"
                     /translation="MEVFQREASSVDVATSLRELREARGISMRTLATKSGLSANALSM
                     IERGKTSPSVSTLYKLADALGVSITAFFGTETEKKQVVFLKQDERTRMSFTRGLFEAL
                     GGEQFVGRVEPFMLTLESGASSGPHSIVHSGHEFVFCLRGQLDYTVEKQVFHLTVGDS
                     LLFASRLQHKWKNPGHTVTNALIVISGFAEGEQPHSMHWKKGE"
     gene            1930..2277
                     /locus_tag="PLE39_17300"
     CDS             1930..2277
                     /locus_tag="PLE39_17300"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE70143.1"
                     /translation="MVTPTLQQEITQLEANFCAALSDPNRLLILYALNDKPMNVTELG
                     NELGLNQPTTSRHLKVLRERGLVVAVRMGTTITYHLSDPRLIQALDLLRSVMRERLAY
                     QASLINEISQENV"
     gene            2274..3710
                     /locus_tag="PLE39_17305"
     CDS             2274..3710
                     /locus_tag="PLE39_17305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HNE70144.1"
                     /translation="MKKRFPLWTIGLVGIILLILVPVLYFLPRTQSKNDPAAHLPTKP
                     VHVDHKDIVKGEFKTGQDVTRACLECHEDAATEFMKTTHWTWESKAFDVPWREEDVTI
                     GKINQINNFCIGSQGNENKCMSCHAGYGWEQGKEQQQSNEENVDCLACHADTGSYGKG
                     LFGNPAEGVDLLAAAQSVRAPTRENCGKCHFDGGGGNGVKHGDLDESLYFPTQNNDVH
                     MGGDLNMQCTDCHWTQDHQILGRMIADNYTIDPKEQVSCEQCHVEQKHEDERINTHLS
                     AVACQTCHIPALALEDPTKVYWDWSQAGQEGRTDDHFTYLKIKGEFIYDKNFAPAYMW
                     FNGNNEYRYILGDPLGQDGITYINKPAGSIDDKSAKIFPFKLHVAKQPYDVNNNYLLQ
                     PITSGKDGFWTTFDWNSAFELAAPITGLEYSGQYDFTTTYMYWATTHMVQPADQALQC
                     DSCHGENGRLDWEALGYPGDPIEWGGRQ"
     gene            3778..6465
                     /locus_tag="PLE39_17310"
     CDS             3778..6465
                     /locus_tag="PLE39_17310"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNE70145.1"
                     /translation="MNKYKLLTVVGFLVLVLAIGIGTVFASPQPAPTQQESILHPNFI
                     LLDKNGVNVIENNSPVSTTQTCGQCHDTAFIESHAFHADLGLADYQETKDLNASNGLF
                     GEWDPLTYRYLSQDGDERLDLSTAEWLMLNGDRVVGGGPATTSRNGIALTDVKANVEN
                     PETAILNNGTAATWNWDESGTMEMNCFLCHLASPNTEARSEAIHAGEFGNANTATLLN
                     LNIVTTSADGWAWNAEAFNENGELKSEVLGIQDPTNENCAACHGEVHPNSGEPLTVNQ
                     CDLEYPQTATTGQVVSGQRINASGVNLTGKNDLDRSWDIHAERQLQCTDCHYALNNPA
                     HASELQSSNPEHLIYDPRSLEINEYLQRPDHNFARGESAQFNVSPEYKGTMRRCENCH
                     DASKGHSDWLPYIDTHMEAVACETCHIPQMYAPAIQAYDWTVITTNGNAVSSCRGVEG
                     TPNEVTTLVTGYEPVLLNRTNGDGNTLLAPYNLITSFYWVYDDANGNKRPVRLVDLET
                     AYLDNGNYASDIITAFDTNNDGKLSTAELKIDSSAKEEAVKAKLLGLGLNNPRIEGMV
                     QPYSINHNVTRGEYAVNDCKACHDADSRMTQPIKLADNAPVMPVFDGNNNVNGTGDLV
                     KGADGTLYYQPNPAKDNMYVFGTSRVNWVDWFGAIAFAGSLLGVIGHGTLRIFSSRKQ
                     VKGHVRTERVYMYEPYRRFWHWLQTSAIVLLLFTGLIIHRPDIFGAFSFRGVVTVHNV
                     LAVILVINAAMSLFYHLATERIQEFIPRPYGFFDDAMVQAKYYINGIFKGEGHPFEKL
                     PTSRMNPIQKATYFAILNVLLPLQIITGALMWAVQKWPEIAGGLPFLAPFHSLVAWMF
                     GTFIIVHVYMTTTGATPLEATRAMITGYEEVEVHTHNEG"
     gene            6488..7033
                     /locus_tag="PLE39_17315"
     CDS             6488..7033
                     /locus_tag="PLE39_17315"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNE70146.1"
                     /translation="MTTQTKKSIFSRPEKSYVHPYFGGIVLGIVLFLALFLTGNGLGS
                     SGATARIDAAIVDAVSPSHVDNNPYLLKMAGGDKNPFDDWIVPVFVGALLGGFSSGFF
                     NGRLKFMTTKGPNISNQTRWLMAFLGGVIFLYGARMARGCTSGQALTGGTVLSAGSWV
                     VMFAIFGGAYGLAYFVRKLWN"
     gene            7120..>7518
                     /locus_tag="PLE39_17320"
     CDS             7120..>7518
                     /locus_tag="PLE39_17320"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNE70147.1"
                     /translation="MTFPLPSLVAVSAANPWTYILFGVIGFAFGYTLEMSGFGDSRKL
                     AAQFYFTELTVLKVMFTAIVTAMVLLFGAVGLGILDFSQVWVNPTYLWSGLVGGLIMG
                     VGFIIGGFCPTTSLASASTGKIDGMMFMLGG"
BASE COUNT         2029 a         2096 c         1670 g         1723 t
ORIGIN      
        1 catatattta tgatatttat ctagtatatt gacattcgct cgcctcttgg ttatactgcc
       61 atgcatgaaa tcaactcgcg acaagatttt acaaaccttg ctaaaaaagc cacggtccac
      121 cattaatgac ctggcagaag ctgttggcat caacccaatt tctgtgcgcc accaccttac
      181 caacctccaa atggagggac tggtggaagg ccaggaagag cgtcatgggg ttgggcgtcc
      241 gcgcttggtt tatgtcctca cctctgaagg catggagcac tttccaacac gctatatgct
      301 gttgactaca cgactactct cccaaatgaa agaaacgatg cctggccctg tggttgccaa
      361 attattcaac cagattgcgg aagatcttgc cagccaatac gccaacgaca tgaaacacct
      421 gagtatggaa gagcggcttg acttcgtcaa agacatgttg gctcaggaag gctttaccgt
      481 ggaatgggaa aagaaagatg gaaattacca gatccatgaa atttcatgtc cgtattacca
      541 gattggtgtt gcccaccctg aagtttgcac cgttgaccaa accttgatct caaaaatgct
      601 tgcacttcct gccaacaaag tccaatgcat tctcgatggc ggcgcacact gcacttatgt
      661 cgtccaacct gtagttgaag ttaaaagcaa ataaagagga accatgagcg aagaaaccaa
      721 agaaattcaa tggaccatcc ataaaaccca tcctgaaatg gtaaagaacg cccgagacag
      781 gctttctgaa gtagtagacc ctgaaattgg catgaacatc attcagttgg gtttggtacg
      841 tgatatagag atcgaaaaca acattgccca cctcaaaatg atcctgacca cccctttctg
      901 cccttacggc ccagccatga tcgagatgac caaggccaaa gccaccgaag ggcttaacat
      961 gccagtcaat attgaaatgg gtatggatgt gtgggatttc tccatgatgg aagacccctc
     1021 cgcattggat tggggcatgt acgcctaatt tacaagtcgt ttgatttgtc ttttacaaaa
     1081 aatacgccac caaattggtg gcgtattttt ttatctttat catccaaatc tgaactattc
     1141 gccttttttc cagtgcatgg aatgaggctg ttccccttct gcgaaaccag aaatcacgat
     1201 caaagcattc gtaaccgtgt gcccggggtt tttccattta tgctgcaaac gggaggcaaa
     1261 caaaagactg tcacccacag tcaagtggaa gacttgtttt tcaacggtgt agtccaactg
     1321 accgcgaaga caaaaaacaa actcatgccc actgtgaaca atgctatgcg gtccgctgga
     1381 ggctccactc tccagagtca gcatgaacgg ctcaacccgc ccgacaaact gttccccacc
     1441 caaggcctca aacaaacccc gcgtaaatga catacgagtg cgttcatcct gcttcaaaaa
     1501 aacaacctgt ttcttttctg tttcggtacc aaaaaacgca gtaattgaaa cccccaaagc
     1561 atctgccaat ttatacaaag tactcaccga aggtgaagtc ttgccacgct cgatcatgct
     1621 caaggcgttg gctgacaatc cgcttttcgt agcgagtgta cgcattgaga taccacgcgc
     1681 ctctcgaagt tcacgcaaac ttgttgccac atctacagaa ctcgcttcac gttgaaatac
     1741 ttccatttcc aaactccttt tgcgtggtat cgaagactgt aaccctacca aatctgcccg
     1801 aattatacac catcctaact ttatgtgaca ttaaacgaaa cttaatatga cacacaccac
     1861 tattgtacaa tctgtcacaa tattatgatg ggattgaaat attcgtttat tcgcatatag
     1921 gcaggtacca tggtcacccc tacccttcaa caagaaatca cccaactgga agctaatttt
     1981 tgcgctgcac tgtcagaccc aaatcgctta ctaattctat atgccctcaa cgataagccc
     2041 atgaacgtca ccgagctggg aaatgaactc gggttgaatc agcccactac gtcgcgccat
     2101 ctcaaggtcc tgcgcgaacg agggctggtg gttgccgtcc gcatgggtac cacaatcacc
     2161 taccatttat ccgatccaag attaattcag gcgcttgacc tgttgcgaag tgtgatgcgc
     2221 gagcgacttg cctaccaagc cagcctgatc aatgaaattt cacaggagaa tgtatgaaaa
     2281 aacgcttccc actctggacg ataggtctgg tcggcatcat tttgttgatc ctcgtccctg
     2341 tcctttactt cctcccacgc acccaatcaa aaaatgatcc tgctgcacac ctaccaacaa
     2401 agcctgtaca cgtggatcac aaagacattg tcaaaggtga gttcaaaacg ggacaagatg
     2461 tgacccgagc ctgcctggaa tgtcacgaag atgcggcaac tgaatttatg aagaccacgc
     2521 attggacatg ggaatcaaaa gcctttgatg tgccctggcg cgaagaagat gtcacgatcg
     2581 gcaagatcaa ccagatcaac aacttctgca ttggctcaca aggaaacgaa aacaaatgca
     2641 tgtcctgtca tgcaggttat ggctgggagc aaggcaagga acaacaacag tccaacgaag
     2701 aaaacgtgga ttgtctcgca tgccacgctg atacaggctc ctacggcaaa ggtctgtttg
     2761 gcaatcccgc cgaaggtgtt gaccttttag ccgctgctca aagcgtccga gctccaacac
     2821 gcgaaaactg cggcaaatgc cattttgatg gaggcggcgg aaacggcgtc aaacacggcg
     2881 accttgatga aagcctgtac ttccccaccc aaaacaatga cgtccacatg ggcggtgact
     2941 tgaacatgca atgcaccgac tgccactgga cacaagacca tcaaatctta gggagaatga
     3001 tcgccgacaa ttacacgatc gatcccaagg aacaggtctc ctgtgaacaa tgccatgtag
     3061 agcaaaagca tgaagatgaa cgcatcaaca cccacctctc tgccgtagcc tgccaaacct
     3121 gccacattcc cgcactcgcg cttgaagacc ccaccaaagt ctactgggac tggtctcaag
     3181 cagggcagga gggccgcaca gacgatcact tcacctatct caagatcaag ggtgaattca
     3241 tctacgacaa gaatttcgca cctgcctaca tgtggttcaa cggcaacaat gaataccgct
     3301 acattttggg cgacccgctc gggcaggatg gcattaccta catcaacaaa cctgcgggca
     3361 gcatcgatga caaatccgcg aaaatattcc ccttcaaact acatgttgcc aaacaaccct
     3421 acgatgtaaa caacaattac ctgctccaac ccatcacctc agggaaagac ggcttctgga
     3481 ccacctttga ttggaacagt gcatttgaac tggcagcccc catcacaggt ctcgaataca
     3541 gcggacaata cgacttcacc accacctaca tgtactgggc aacaacccac atggtgcagc
     3601 ccgctgacca ggctctacaa tgcgattcct gccatggcga aaatggccgc ctcgactggg
     3661 aagcccttgg ctaccctggc gacccgatcg aatggggagg aaggcaataa atcaggaatt
     3721 gtgaattagg aattaagaat cacttaattc ctaattccta atccacaagg atgaattatg
     3781 aacaaatata aacttctcac cgtagtcgga tttctagtcc ttgtccttgc cattggaatt
     3841 ggaacggtct ttgcttcgcc tcagcctgct cccacccaac aagagagcat ccttcaccca
     3901 aactttatcc tgctcgataa aaacggcgtg aatgtgatcg aaaacaacag cccagtctcc
     3961 accacccaaa cctgtggaca atgccatgac accgcattta ttgaaagcca tgccttccat
     4021 gcagaccttg ggcttgcaga ttatcaggaa accaaagacc tcaacgccag caacggcctc
     4081 ttcggtgaat gggatccgct aacctaccgc tatctttcac aggatggtga tgaacgcctt
     4141 gacctctcca ccgctgaatg gctgatgctc aacggagacc gtgttgtcgg tggcggacca
     4201 gccactacat cccgcaatgg cattgcattg accgatgtca aagccaacgt tgaaaaccct
     4261 gaaacagcca ttctgaacaa cggtactgcc gccacctgga attgggacga atcgggcacc
     4321 atggaaatga actgcttcct ctgccatctt gcatcaccca ataccgaagc ccgctccgaa
     4381 gcgatccatg caggtgagtt tggcaacgcc aacacagcca cactgctcaa cctaaatatc
     4441 gtcaccacat ctgctgatgg ctgggcttgg aatgccgaag ccttcaacga aaacggcgaa
     4501 ctcaagagcg aagtccttgg aattcaagac cccaccaatg aaaattgcgc cgcctgtcac
     4561 ggagaggttc atccgaattc tggtgagccg cttaccgtaa atcaatgtga cctcgaatac
     4621 ccacagactg caaccacagg ccaggtcgta agcggacagc gcattaatgc ctcaggcgta
     4681 aacctgactg gaaagaacga tctcgaccgc tcatgggata ttcacgcgga acgccagttg
     4741 caatgcaccg actgtcacta cgcattgaac aaccctgctc acgccagcga actgcaaagc
     4801 agcaaccctg agcatctgat ctatgacccg cgctctttgg agatcaacga atacctgcag
     4861 cgccctgacc ataatttcgc acgcggcgaa agcgcacaat tcaatgtctc tcccgaatac
     4921 aaaggcacga tgcgccgctg tgaaaactgc cacgacgcca gcaaaggcca ctcagattgg
     4981 ctcccctaca tcgacaccca catggaagct gtcgcctgcg aaacctgtca catcccgcag
     5041 atgtatgccc ctgccattca ggcctatgac tggaccgtca tcaccaccaa cggcaacgct
     5101 gtttcctcct gccgcggcgt ggaaggcact cccaatgaag taacaaccct cgttacaggg
     5161 tatgaacccg tattgctcaa tcgaaccaac ggcgatggca ataccctgct ggctccgtac
     5221 aatctcatca cttcgttcta ctgggtgtat gatgacgcca atggcaacaa acgccctgtc
     5281 cgtcttgtgg acctcgaaac agcctatctc gacaacggca actacgccag cgacatcatc
     5341 actgctttcg acacaaacaa tgatggcaag ttgagcactg ccgaactgaa gatcgactct
     5401 tccgcaaagg aagaagccgt aaaagcaaaa ctgctcgggc ttggtctgaa caatccgcgg
     5461 atcgaaggta tggtgcagcc ctacagcatc aaccacaacg tgacccgcgg tgaatatgca
     5521 gtcaatgact gcaaagcctg ccacgacgca gattcacgca tgacccaacc catcaaactt
     5581 gcggacaatg cacccgtcat gccagtattc gatggcaata acaatgtcaa cggcactggt
     5641 gatctggtca aaggcgccga tggcacgctt tattaccagc ccaatcccgc caaagacaat
     5701 atgtacgttt tcggcaccag ccgcgtaaat tgggtggatt ggtttggagc aatcgccttc
     5761 gctggctcgc ttttaggtgt gatcgggcat gggacgctga gaatcttttc gtcgcggaaa
     5821 caggttaagg gtcacgtaag aaccgagcgg gtttacatgt acgaaccgta tcgtcgcttc
     5881 tggcattggc tccagacatc cgccatcgtt ctcctactct tcactggtct catcatccac
     5941 aggccagata tctttggagc attttcattc cgcggtgtgg tgacggtaca caatgtactt
     6001 gcggtcatcc tcgtcatcaa tgcggccatg tcgctctttt accaccttgc caccgaacgt
     6061 attcaggaat tcatcccgcg cccttatggc ttcttcgatg acgccatggt gcaggcaaag
     6121 tattacatca acggcatctt caagggcgaa gggcacccat ttgagaaact gccgaccagc
     6181 cgcatgaacc caattcagaa agccacctac tttgccattt tgaatgtgct tctgcctctg
     6241 cagatcatca caggggcatt aatgtgggct gtgcaaaaat ggcccgaaat tgctggcgga
     6301 cttcccttcc ttgctccgtt ccactcgctc gttgcgtgga tgttcggcac gttcattatc
     6361 gtccatgtgt acatgaccac cactggtgca acaccgctcg aagcaacccg cgccatgatc
     6421 actggctacg aagaagtgga agtgcatacg cacaacgaag gatgaaagat aaaggatgaa
     6481 taaaaccatg actactcaaa caaaaaaatc aatcttcagt cggcctgaaa aatcgtatgt
     6541 gcatccatac tttggcggaa ttgtgcttgg catcgtcctg ttcctggcgc tctttctgac
     6601 aggcaatggc cttggctcat caggcgcaac cgcccgcatt gacgccgcca ttgtagatgc
     6661 agtctcccca tctcacgtgg acaacaaccc ctacctgttg aaaatggcag gcggtgacaa
     6721 aaaccccttt gatgactgga tcgtgccagt tttcgtcggc gctttactcg gcggattctc
     6781 ttcaggcttt ttcaatggcc gcttgaaatt catgaccacc aaaggcccca acatttcaaa
     6841 ccaaacccgc tggttgatgg ccttccttgg cggtgtgatc tttctctacg gtgcgcgcat
     6901 ggcccgtgga tgcacatcgg ggcaggcact cacaggcggc acggtgcttt cggctggctc
     6961 ctgggtggtg atgtttgcca tctttggcgg tgcgtatggg ctggcatact ttgtcagaaa
     7021 actttggaac taaaagagtt gcaggttgta agttacaggt tgaagaataa cctgcaacct
     7081 tcaacctaaa accttcaact cacacacagg agaaacaaaa tgacattccc tctcccctct
     7141 ctcgttgcag tcagcgcagc caacccgtgg acatatattc tcttcggtgt cattggcttt
     7201 gcattcggtt acaccctcga aatgtctggt tttggtgact cacgcaaact tgcggcacaa
     7261 ttttatttca ctgaattgac cgtgctcaag gtcatgttca ccgccatcgt caccgccatg
     7321 gtcttgcttt tcggagcggt cggcctcgga attcttgatt tcagtcaggt ttgggtcaac
     7381 cccacctatc tttggtctgg ccttgtcggc gggctcatca tgggcgttgg ttttatcatt
     7441 ggcggttttt gtcccacaac ttcactggca tctgcctcca caggcaaaat tgacggcatg
     7501 atgttcatgc tcggtggt
//
LOCUS       DAOQJC010000163         7494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_5403_length_7494_cov_8.071381, whole genome shotgun sequence.
ACCESSION   DAOQJC010000163 DAOQJC010000000
VERSION     DAOQJC010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5403_length_7494_cov_8.071381"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1518)
                     /locus_tag="PLE39_17325"
     CDS             complement(<1..1518)
                     /locus_tag="PLE39_17325"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE70148.1"
                     /translation="MTTTYDIAIIGAGAVGCAMARELSRYQLNIALLEANSDVGMGTS
                     KASTAIWHTGYDAKPGSLEASLLKRSYKLMQEYLPEANIAHELLGGLLIAWNQDQLDT
                     LPKLLNQAHANGEMDVRIISAEEIYQREPHINKGALGGLFVPGEGILCTFSVPIAMAY
                     QAVLNGVELFLNFQVKDIQRQDTGDGPIWSIASGSSSVQARYVINAAGLFSDEINAHF
                     GKTSFKVTPRRGQLIVYDKMARPLVNHVLLPVPTSITKGVLISPTVYGNILLGPTAED
                     LPASRKSATETTESGLKFLLEKGKQILPQLLEEEVTATYSGLRAATEHSDYQIEMDSA
                     LRYLCLGGIRSTGISGAMGIAEYGVELLQGAGLALKPKPEFKKVKLPSIGQASMRPHQ
                     NAEMIARNAAYAEIVCHCERVSRGELMDALNAPVPAASVDALRRRTRASQGRCQGFNC
                     HAALTRLAAAEPAPQITFEKDTARPVPVVQKDVLIVGGGPAGLSAAVELKKQGIQN"
     gene            complement(1564..3033)
                     /gene="glpK"
                     /locus_tag="PLE39_17330"
     CDS             complement(1564..3033)
                     /gene="glpK"
                     /locus_tag="PLE39_17330"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNE70149.1"
                     /translation="MKYLLGIDQGTTQTTAVIVNEMGEMLEKNSAQLPARFPQAGWVE
                     QEPADIVRTVIEACAPLLDKYDISAVGFDNQGETFVVWDAETGEAVTPAIVWQDTRGQ
                     SICDALAPGVDPNLLRQKTGLLLDSYFSAPKLKWVFENHPEIRKRAHDGRLKFGTTET
                     WVIWKLSKGQLHVTDPSTASRTLLFDMNTFQWDDDLLKLFDVPRSMLPEVKPSAGHIG
                     DVDFGNRKPLPLHALLVDQQAALFGQACFNAGEMKCSFGTGSFLLMNIGDKPKLSNNG
                     LLTTVGWNFNGQTAYAFDGGIFVTGSAVQWLRDNLKFIPDSASSHNAAQRSKDAGVVV
                     IPALQGLAAPHWRTDVQGAMFGLNRSTTSDDIVRATLDGIACRVYEVVTAMSQDLGQR
                     PPHLKVDGGPSGNPYLMQMIADLLNIEVRVSAALEATAIGIANLAGVSALGTSFEALA
                     QNWKSETSYQPQMKKEDREKKLEQWGRALNAVKAFHGNG"
     gene            complement(3225..4928)
                     /locus_tag="PLE39_17335"
     CDS             complement(3225..4928)
                     /locus_tag="PLE39_17335"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNE70150.1"
                     /translation="MAGNKSQAHDKQVLHKLGYAQELSRRMSGFSNFAISFSIICILA
                     GGISAFPAAFNALGSGGAFLIWLVGGVLAMSVAVGMGQIASSFPTAGGLYHWSSHLGG
                     KFWGWATAWFNLIGLICVVSSVDVLLYTVFFKDLLLGTALGVDVSGFGTMHQTVFLII
                     ALGTQALLNHYYIDITTKLTDWSGYVILGLTVILIITLFAFSPVALDFSRLTSFQNVT
                     GDAGGAVVPFRTESVAFAFLLGLSYVCYTITGFDASAHTSEETQDAQVNVPKGMWTAV
                     FWSWVFGLVAVAAYVLTMPDLNEAAAAGWGSFFYMWGASRMPSWLSIFLAVGMVLVNY
                     VCALAGLTSTSRMMYAFARDGGLPASAALSKVSTVYRTPGTAVWTSAILAFASTLYAP
                     AYLILAVACAVFLYLSMVMPIAAGLMAEGGPKWKEKGPFNLGGFSKANAVLAIIFGIV
                     LAISGFFPPNEKVFYFTIVFVVALLGFWSKKTAPIGVVVAVVGLALSFIPISEENVMH
                     FLIPDATTSYIAIGVAVLAAIITFMTGGEDNRFEGVPEGDKIKERQAKIAEIEKQFGE
                     H"
     gene            5155..6312
                     /locus_tag="PLE39_17340"
     CDS             5155..6312
                     /locus_tag="PLE39_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70151.1"
                     /translation="MDSLDTLKLLSSQMTFEPDGEPRINSDTPTCFSPKERDHAFTHP
                     AQLPNGQKIVLLKTLLTSACERDCFYCPFRAGRDFRRATFKPDEFASLFSKMNRGGMA
                     EGIFLSSGVAGGGVRTQDKLLDTADILRRKYQFKGYLHLKMMPGSEKDQVYRAMQLAD
                     RVSVNLEAPNTERLAKLAPHKVFIEELLRPLRWVEEIRRSVPAYKFWNGRYPSTVTQF
                     VAGGSDESDLELLNTTNWLMKNVRLKRAYFSAFHPIQDTPLENKPAVDPMREHRLYQA
                     SFLLRDYGFDLEDLPFTKDNNLPIHADPKQAWAQMNLLHNPTEINKADKHELLRIPGI
                     GLKGAEAILSARRAYRLRDLASLKKLGIIAERAAPYILLDGRKSAYQLRIF"
     gene            complement(6385..>7494)
                     /locus_tag="PLE39_17345"
     CDS             complement(6385..>7494)
                     /locus_tag="PLE39_17345"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNE70152.1"
                     /translation="VYGAWGDSYDNHSLRDLVAQRYGEPLDDEDIMNLAELGFLSRHH
                     IPPLDKPEDIELEMQIGTRMLQEAARANGWQVGEVDAVLIGAGGPVIPDYVEQICRRA
                     GIPETALKVSVHKACDSSVAGLHLALNPALTRQGHINIAEELLGKKVLVGGIEGLSRF
                     TSHSHDKYALQLFANAAGVIGVIPGETFKFLVGRNHEAFDGEGVLAIHMYYPHPGKNV
                     GDSLIEVTQENPSHIRVAGLMHEPKDGSPISMAGPMGMVKLFVRTGVKVVTEVFYEYA
                     ELMKKLGTPEKQIAVGIVHHANYKINQLKAKQLQSAGIEFPMPWLLSDFGNVSAASNM
                     IAFLRQLPNIKPGDNVLFDGFGAGTYYDVLAVTLG"
BASE COUNT         1866 a         2028 c         1821 g         1779 t
ORIGIN      
        1 gttctggatg ccttgcttct tcaactcaac cgccgccgaa agccccgcag gtccaccgcc
       61 gacgatcaaa acatcttttt gcacaacggg cacaggacgt gccgtgtctt tttcaaaagt
      121 gatttgaggc gcaggttctg cagccgccag ccttgtcagc gcggcatggc aattgaatcc
      181 ctggcacctt ccttgcgagg cacgggtgcg ccttcgcagg gcatccacac tggcagcggg
      241 aacgggcgca ttcaaagcgt ccatcagttc accgcgtgaa acccgttcgc agtgacaaac
      301 gatctctgca tacgccgcat tccgcgcgat catctctgcg ttttgatgcg ggcgcatcga
      361 agcctgccca atgctcggga gttttacttt tttgaattcg ggcttcggtt tcaatgcaag
      421 cccggcaccc tgcaataact ccacgccgta ttcggcaatg cccatcgcac cggaaatgcc
      481 cgttgagcgg attccaccca aacacagata acgaagcgcc gaatccatct cgatctgata
      541 atcgctgtgt tccgtcgccg cgcgcaagcc tgagtaggtc gccgttactt cctcctccaa
      601 caattgcggc aggatctgtt tgcccttttc aagcaaaaac tttaatccgc tttcggtcgt
      661 ttcggttgca gatttacgcg aagcgggcag gtcttctgcg gtgggcccca gcaaaatatt
      721 tccgtatacc gtcgggctga tcaacacacc ttttgtgatg gatgtcggca ccggcagcag
      781 tacgtgatta acaagcggac gcgccatctt gtcatagacg atcaactgtc cgcgtcgcgg
      841 cgtgaccttg aaactggtct ttccaaaatg ggcattgatc tcgtctgaaa acaatcccgc
      901 cgcattgatg acatagcggg cctgcaccga cgacgagccg ctggctattg accatatcgg
      961 gccgtccccg gtgtcctgcc gttgaatgtc ctttacttga aaattcagga acaactccac
     1021 gccgttcaac acagcctgat aagccatcgc aatgggaacg gaaaacgtgc aaaggattcc
     1081 ctccccgggc acaaagagtc cgcccaatgc gcctttgttg atatgcggtt cacgctggta
     1141 gatctcttcc gcagaaataa tgcggacgtc catttcaccg ttggcatggg cctgattcaa
     1201 cagtttgggc agtgtgtcca gttgatcttg attccatgcg atcagcaagc ctcccaataa
     1261 ttcgtgggcg atgttcgctt cgggcaggta ctcctgcatc agcttgtaac tgcgcttgag
     1321 cagactcgcc tccaatgaac ctggtttcgc gtcataccct gtatgccaaa tggccgtact
     1381 ggctttggat gtgcccatgc cgacatctga gttggcttcg agcaatgcaa tgttgagttg
     1441 ataacgggaa agctcccgcg ccatggcaca ccccactgcc cccgcaccga tgatggcaat
     1501 atcgtaagtc gttgtcatag tgatttccca gggcaggcaa cagggtctgc tgcctgctgt
     1561 ctatcatcca tttccgtgaa aagccttcac tgcgtttagc gcccttcccc actgctccaa
     1621 tttcttttcc ctgtcttctt ttttcatctg tggctggtag gaagtttccg atttccaatt
     1681 ctgtgccaat gcttcgaaac ttgtccccag ggcagataca ccggccaggt tggcaattcc
     1741 aatggcagtg gcttccagcg ccgcagaaac gcgcacttcg atgttcaaca agtccgcgat
     1801 catttgcatg aggtatggat tccccgatgg gccgccatcc actttgagat gcggcgggcg
     1861 ctgaccgagg tcttgagaca tggccgtaac cacttcgtac acgcggcagg caattccatc
     1921 caacgtggcg cggacaatgt catcggaggt ggtgctgcgg ttcaagccga acatcgcgcc
     1981 ttgtacatcg gtgcgccagt gcggggcggc gagtccttgc aaggcaggga tcaccaccac
     2041 acctgcatct ttcgaccttt gggcagcatt gtggctggaa gcagaatcgg ggatgaattt
     2101 caagttatcg cgcagccatt gcaccgccga gcccgtgacg aagattccgc catcaaaggc
     2161 ataggctgtc tgcccgttga aattccagcc gaccgtggtg agtagtccat tgttcgagag
     2221 tttgggtttg tcgccgatgt tcatcaacag aaaactgccc gtgccgaagg agcatttcat
     2281 ttcacctgcg ttgaaacatg cctgaccaaa caaagcggcc tgctgatcga ccaatagcgc
     2341 atgcaacggc aatggctttc tgtttccaaa atccacatca ccaatatgcc ccgctgacgg
     2401 ttttacttcg gggagcatgc tgcgaggtac gtcgaataat ttcaacaggt catcatccca
     2461 ttgaaaggtg ttcatgtcga agagcagagt gcgcgaagca gtggaaggat cagtgacatg
     2521 aagctgccct ttgctcagtt tccagatgac ccaggtctct gtggttccga atttcaacct
     2581 gccatcatgg gcccttttgc gaatctcggg atggttttcg aacacccatt tgagtttggg
     2641 cgcagaaaag taactgtcga gcaggagacc tgtcttttgg cggagcaggt tcgggtccac
     2701 accgggagcg agcgcatcac agatggattg tccacgggta tcctgccaca cgatggcggg
     2761 agttaccgct tcacccgtct ctgcatccca aacaacgaag gtctcaccct ggttatcaaa
     2821 accgaccgct gaaatatcgt acttgtcgag caggggcgca caagcctcaa ttacagttcg
     2881 gacaatatcc gcgggctcct gttccaccca gcctgcctgc ggaaaacgcg cgggcaattg
     2941 agcagagttc ttttcaagca tctcccccat ttcattaacg atcactgctg ttgtttgtgt
     3001 agttccttgg tcgataccga gcaggtactt cataagcatc tccttttatg acagccgatt
     3061 cgtatttttt gacttgcatt tccctttgtt ttatgtcaca atatgtttgt tccagttagg
     3121 gtgcccccct caccctggct ggaacatttt ttattcttta actaaaaaga taagagcggt
     3181 atccaattgg ataccgctct tgattttact tcacgctgaa tggtttagtg ttcgccaaat
     3241 tgcttttcga tctcggcgat cttggcctgg cgttccttga tcttgtcgcc ttcggggaca
     3301 ccttcgaaac gattatcttc accaccggtc ataaaggtga tgattgcggc cagaacagcc
     3361 acaccaatgg cgatatagga agttgtggca tctggaatga ggaagtgcat cacattttcc
     3421 tcagagatgg ggatgaaact caatgccagc cctacaacag ccactacaac gccgatcggg
     3481 gcggtcttct tagaccagaa acccaacaag gcaacgacaa agacgatcgt aaagtagaag
     3541 actttttcat tgggcgggaa gaaaccgctg atcgccaata caataccaaa tatgatcgca
     3601 agaacagcat ttgccttgga gaatccgcca agattgaacg gacctttttc cttccacttg
     3661 ggtccgcctt cagccatcaa acctgcggcg atcggcatca ccatggatag gtaaaggaat
     3721 acagcacagg cgactgccaa aatcaggtaa gcgggagcat acagggtgct tgcaaatgca
     3781 agaatggcag aagtccacac agcggtacca ggggtacggt aaacagtgct taccttggag
     3841 agggcagcgg aagccggcaa gccgccatca cgggcgaaag catacatcat gcgagaggtg
     3901 gaagtgagac cagccaaagc acagacgtag ttcacaagca ccatgccaac ggcaaggaag
     3961 atgctcaacc aactcggcat gcgggatgca ccccacatat agaagaatga tccccagcct
     4021 gcggcggcgg cttcattcag gtctggcatc gtcagcacat aggctgcaac agccaccaag
     4081 ccaaataccc aggaccagaa cacagcggtc cacatgccct tcggcacgtt tacctgagcg
     4141 tcttgggttt cttcagaggt gtgcgcagaa gcatcaaaac ctgtgatggt gtaacaaacg
     4201 taggaaagac caagcaggaa ggcaaaggcc acgctttcag tgcggaaagg aaccactgcc
     4261 ccacccgcat cgccagtcac gttttggaag gaggtaaggc gggagaaatc caaagcaaca
     4321 gggctgaagg caaagagggt gatgatcaaa atcacggtca agccaagaat gacataacca
     4381 ctccagtcgg ttaacttggt ggtgatgtca atgtagtaat ggttcaaaag cgcctgagtt
     4441 ccaagggcaa tgatcaaaaa gaccgtttgg tgcatggtgc caaaacctga aacatccaca
     4501 ccgagggcgg tgccgagcaa gaggtctttg aagaacacgg tgtagagcag cacgtccacc
     4561 gaggagacca cacagatcaa accaatgaga ttaaaccagg cagtagccca accccagaat
     4621 ttcccgccga ggtgggaact ccagtgatac aaaccgcccg ccgtcgggaa ggaagatgcg
     4681 atctgtccca tgccaacagc cacgctcatg gcaagaacac caccgaccag ccagatcagg
     4741 aatgcaccgc ctgaaccaag ggcattgaaa gcagcaggaa aagcagaaat accgccagcc
     4801 aaaatacaga tgattgaaaa tgaaattgca aagttcgaga agccgctcat acggcgtgaa
     4861 agctcttgtg cataccccag cttatgtagt acctgtttgt cgtgcgcttg acttttgtta
     4921 ccagccattt tcgtaattct ccttgaaatg atttttttac atcaagaaac cgaacttacg
     4981 aagagcagtt tattaactat caacgataca catcctcctc ctccctttcg ggagcttcct
     5041 ctgaatggta tgtggtgata acactgacaa taggacaaag gtacgaatta attcctgcca
     5101 ttatgacaca aaatgcctct tgataaaata gaccaaatgt tctaaaatga atatatggac
     5161 tctcttgata ctttaaagtt actctcctca caaatgactt tcgagccaga tggagaacca
     5221 cgcatcaact ccgacactcc cacctgcttc agtccgaaag aaagggatca cgcctttact
     5281 catcccgccc aactgccaaa cggacagaag atcgttctat tgaagacact gctgacctct
     5341 gcgtgtgaac gcgattgctt ctattgtcca tttcgtgcag gcagggattt tcgccgtgcc
     5401 acgtttaaac ccgatgaatt cgccagcctt ttcagtaaaa tgaatcgggg cggaatggct
     5461 gagggtatat ttctcagctc cggggtagcg ggaggcggtg ttcggactca ggataaactc
     5521 ctcgatacgg cagatattct ccgcaggaaa tatcaattca aagggtatct ccatctaaaa
     5581 atgatgcctg gctcggaaaa agatcaggtc tatcgcgcca tgcagcttgc agaccgtgtt
     5641 tcggtcaatc ttgaggctcc caacacggag cggcttgcca aacttgcccc ccacaaggtc
     5701 tttattgaag aattgcttcg ccctctgcgc tgggtggagg agatccgcag gagcgtgcct
     5761 gcctacaaat tctggaatgg ccgctaccca tctaccgtga cacagttcgt ggcaggcggt
     5821 tccgatgaaa gtgaccttga gttattgaat acaacaaact ggttgatgaa gaatgtaagg
     5881 cttaagcgtg cttatttctc tgcatttcat cccattcagg atacgccact tgagaataaa
     5941 cctgctgttg accccatgcg tgaacatcgt ctttatcagg cgtcgttcct cctgcgtgat
     6001 tacgggtttg atctggaaga cctgcctttt acaaaggaca acaaccttcc gatccatgct
     6061 gatcccaaac aggcttgggc gcaaatgaat ctgctgcata atccaaccga aatcaacaag
     6121 gctgataaac atgaattact tcgcatcccc ggtattggtt tgaagggtgc agaagccatc
     6181 ctttctgcaa gaagggcgta tcgcttgcgc gatctcgcat cactgaagaa gcttggcatt
     6241 attgccgagc gtgccgcccc ctatattctt ctagatggaa gaaaatctgc ataccaactt
     6301 cgtatttttt aattaggtca aatcggcaaa aaacgggctg caaatggcac atgccacctg
     6361 cagcccgttt ttacatgaaa tcatttaccc cagagtcaca gccagtacat cataatacgt
     6421 gcccgcccca aacccatcga aaaggacatt gtcgccaggt ttgatgtttg gcaattggcg
     6481 gagaaatgcg atcatattgc ttgccgcact gacgtttcca aaatcgctca agagccacgg
     6541 catggggaat tcaatgccgg cgctttgtag ttgtttcgct ttcaattgat tgatcttgta
     6601 attggcatgg tggacaatgc caactgcaat ttgcttttcc ggggtgccga gttttttcat
     6661 caactcggca tattcgtaga acacctcagt caccacttta acacccgtac ggacaaacaa
     6721 cttgaccatt cccattgggc cagccattga gatcggagag ccgtcttttg gttcatgcat
     6781 caaaccagcc acacggatgt gcgatgggtt ttcctgtgtc acttcgatca aagaatctcc
     6841 cacatttttg ccagggtgcg gataatacat gtgaatggca aggacacctt ccccatcaaa
     6901 ggcttcatga ttcctgccaa ccaaaaactt gaatgtttcc cccgggataa ccccaatcac
     6961 accagctgca ttggcaaata attgcagggc atatttatcg tgtgaatggc tggtgaaacg
     7021 gctcagtcct tcaattccac ctaccaaaac ctttttgccg agtagttctt ctgcgatatt
     7081 gatatgtccc tggcgcgtca gggctgggtt tagggccaaa tgcaaacccg ccacagagct
     7141 gtcacaggct ttgtgtacac ttaccttcaa tgcggtttcc gggatgcctg ctcggcgaca
     7201 gatctgttcc acataatccg ggatgacagg tccgcctgca ccgatcaaca ctgcatccac
     7261 ttcgccaact tgccagccgt ttgcgcgggc ggcctcctgc agcattcttg tgccgatttg
     7321 catctccaac tcgatatcct caggtttgtc caatggagga atgtggtgac ggcttaggaa
     7381 tcccagctct gccagattca taatatcctc gtcatccaag ggctcgccat atctttgcgc
     7441 caccaagtca cgcaggctgt ggttatcata actgtcaccc catgcgccat atac
//
LOCUS       DAOQJC010000164         7419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_5488_length_7419_cov_10.350896, whole genome shotgun sequence.
ACCESSION   DAOQJC010000164 DAOQJC010000000
VERSION     DAOQJC010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7419
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5488_length_7419_cov_10.350896"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..324
                     /locus_tag="PLE39_17350"
     CDS             73..324
                     /locus_tag="PLE39_17350"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HNE70153.1"
                     /translation="MTLQQITIEIPEKVLLAEKTDAESFGREILILAAVKLFEIGRLS
                     SGRASELAGMSRIEFLLNLNRYKVFPFASELDDLENAHA"
     gene            317..802
                     /locus_tag="PLE39_17355"
     CDS             317..802
                     /locus_tag="PLE39_17355"
                     /inference="COORDINATES: protein motif:HMM:NF023276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HNE70154.1"
                     /translation="MPKAISNTSPLLYLYKIGAINLLPQIFDEVWTAESVKNELLAGR
                     SKGYDVPNPADYPWLNIVNPKSMPSEWLALDLGAGELGAMALALENPDRIVLLDDMLA
                     RRTAQVAGLQVWGTLKVLLEAKSQGLIEKIEPYISKLSDAGMWVSAEVKQRILILAGE
                     S"
     gene            928..1470
                     /locus_tag="PLE39_17360"
     CDS             928..1470
                     /locus_tag="PLE39_17360"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE70155.1"
                     /translation="MKKTPVATRKPKRLSTVVKAELRRDALIADLIETRRKIVDVASS
                     LSPTEQDQVFLGIWSMKDLLAHLIGWLEENRKAVKTIRAGKLPEFYAYKDRDWQTYNA
                     YLVSKYKRGDMTELLAKLQVSQQKFIKSIRAVPIEVLEQDFGVRFNRYKVTIVRLLQA
                     ELKDEKVHYAQMEAFRNKAK"
     gene            1580..2068
                     /locus_tag="PLE39_17365"
     CDS             1580..2068
                     /locus_tag="PLE39_17365"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE70156.1"
                     /translation="MEIYIRKATANDYDDLCELFDEVDALHRVNLPHVFQKSTGPARE
                     KDYYLGLIADENIGLFVAEMEEKLAGFVHVLVRDTPVFSIVIPRRYAIVDGIAVKSEF
                     QKHGIGKMLMDKAQEWAETKDAEFMELNVYEFNRNAISFYEGLGYQSSSRKMRKEINK
                     AG"
     gene            2187..3089
                     /locus_tag="PLE39_17370"
     CDS             2187..3089
                     /locus_tag="PLE39_17370"
                     /inference="COORDINATES: protein motif:HMM:NF021537.2"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HNE70157.1"
                     /translation="MPENVSTYQIVDLMPDFWEFHAKNRDADVSEQASQFKRMVVDKH
                     PEAYAARVIGLDPNKPYDEALAERYLLCHERSTAQQVSTMQRISASIARDMSAYEEKF
                     RKVFPDFNYTGSVYFMNSLLGFDGATRTVNGKIALLFGLDTMAWVYGDDLDLQPFFHH
                     EIFHIYHSQFLEDDSDEVAAAVWQEGLAQFVAKSLNPDAEGVNLFGLPKEMPQRSEAM
                     LSTLASGLLSVLDSTSRDDYGRYFFGNNGSEEIPPRSGYYLSYLVVQKLSKKYSLQEM
                     AHSKLAAIKPEIKTALEELANGGR"
     gene            3092..3916
                     /locus_tag="PLE39_17375"
     CDS             3092..3916
                     /locus_tag="PLE39_17375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE70158.1"
                     /translation="MQGETMEAYDDDLGRFEAEGAAPLPDSGNQGYIDHDNARIWYAD
                     YGVGPTVVLLHGGLGHSGNWGYQIPTLIGNGYRTIVIDSRGHGRSTRDDRPYTYELMA
                     SDVLAVMDVLGVEKAALAGWSDGACIALVLAAQVPARVEGVFFFACNMDPSGAKEITE
                     FAPILQRCIGRHIKDYGQLSETPDQFEALSEAVGLMQRTQPNYSANDLAQITVPVAVI
                     HSEFDEFIKLEHAEYLARSIPNADFVYLSGVSHFAPLQRPDQFNDTLLNFLGKVLV"
     gene            4089..4802
                     /locus_tag="PLE39_17380"
     CDS             4089..4802
                     /locus_tag="PLE39_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70159.1"
                     /translation="MTDKPSTTQPIMDRRLLVLLVLSVAFLVIAFLGGQPSSARGFYY
                     AVWLTFSGWLWKKRERLSLWLQARPLPEFPLFVGLGLVMILVEETIAGVAVHLLNVDS
                     VGQLFAVVPLYYFNNLLLLPGFIVGWYVLLKFCAYSRTEVFVLVGLFGLFSEKIYIHV
                     LTIPILGIPLILPTMFTYMAIIMPSILSLRSQSTRPLNRPLRYLLGFVFPIIVSMPFI
                     FIHTLLTNAGLIDPTILTK"
     gene            4872..6722
                     /locus_tag="PLE39_17385"
     CDS             4872..6722
                     /locus_tag="PLE39_17385"
                     /inference="COORDINATES: protein motif:HMM:NF013438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HNE70160.1"
                     /translation="MKKLFMLGLVVLLLLSTAVPVLAGTNASHAAVPNQFVDVPQNHW
                     AVAWINQLYTEGVTGGCSTAPLRYCPDAVVTRAQMAVFLVRVIHGSTFTPPATSVIFE
                     DVPADYWASAWINQLNADGVTGGCSSAPKLFCPEANVSRAQMAVFLLRAKYGSAYSPP
                     ASADVFSDVPADYWARDWIGQLYAEGYTGGCSSNPLQFCPEAFVSRDQMSVFILRAIH
                     GANYVPPPIDQTSLRPFPQHVQYAPGSILPDHRTQQQLDDDVRAFYDYWKANYLIAAG
                     VNADGIPMFRVAFGKNEPDRSATVSEGQGYGMLIVPIMAGYDPDAQAIFDGLWAFARA
                     HPSEGDPRLMDWKVPQTDGNDSAFDGDADIAFGLLLADAQWGSAGLVNYKAEAETMIA
                     GILESTIGPSSHLPMLGDWTDPNGVTYNQYTPRSSDFMLTNFRAYGQATGNPVWDDVV
                     AQSQGVVVAMQANHSPQTGLLPDFIVRKNGSYEPAPVNFLEADTDGAYSYNAGRDPWR
                     LGMDALLSDDAASRASALKISHWAESAVSGSPANFRAGYNLNGTPLPNSDYFTSFFVA
                     PLGVAAMNDSAQQDWLNAVYDSVYATREDYYSDSVTLLCLLVMTGNFWTP"
     gene            6719..>7419
                     /locus_tag="PLE39_17390"
     CDS             6719..>7419
                     /locus_tag="PLE39_17390"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNE70161.1"
                     /translation="MNSIHSQSLVWDAHACFPLNANSDLSELKRYKDSGVNFVSLNIG
                     MDMDSFENVIQVLACFRKYIAAYPDDLVLALAVDDVIGAKASGKLAVAFDLEGSDPLR
                     GDLNLISFYYDLGVRQMLLAYNKDNRASGGCMEGHIGLTDFGKQVIREMNRVGMVVDV
                     THMGYRATMEAFETSAAPVIFSHSNPKSLRDNARNITDEQIKACAKTGGVIAINGIGD
                     FLGGTTSEIMVKHIE"
BASE COUNT         1804 a         1755 c         1925 g         1935 t
ORIGIN      
        1 tgcaaagcga aagttgcaat taaaaatcga agttgaaacg agtaaaatta gcggtggaaa
       61 aaaggagtaa aaatgactct ccaacaaatt actatcgaaa ttccagaaaa ggtacttctc
      121 gcagaaaaga cggatgctga atcttttggg cgtgaaattc tcattttagc ggcagtcaag
      181 ttgtttgaaa ttggcaggct ttcttctggg cgagcatccg aacttgcggg aatgtccaga
      241 attgagtttt tgctcaactt gaaccgctat aaggtttttc cgtttgcctc cgagcttgat
      301 gatttagaaa acgcccatgc ctaaagccat cagcaacact tccccactac tttatctata
      361 caagattggc gcaatcaatt tacttccaca aatattcgat gaagtttgga cagctgaatc
      421 tgtcaaaaat gaattactgg cagggcggag caaaggctat gatgttccaa atccagcaga
      481 ttatccctgg ttgaacatcg tcaatccaaa atccatgcct tctgaatggc ttgcgttaga
      541 tttgggagcg ggtgaacttg gcgcaatggc tttggcttta gaaaatcccg atcgtattgt
      601 tttgcttgat gacatgcttg ctcgtcgtac tgcacaggta gcgggtttac aggtttgggg
      661 aactttgaaa gttttactgg aagcaaaatc tcaaggtttg attgaaaaga ttgaaccgta
      721 tatatcgaaa ttaagtgacg caggtatgtg ggtttccgct gaagtaaaac agcgtattct
      781 tattttggcg ggtgagtctt aaagtcaaaa gcgtgccaac aaagcgtacc tctgacactg
      841 gggactgccg tagggcgggc gcaaatctta agcaggtttc tgcttctgag cagagtccac
      901 gaacgcacaa gatatggaca gtaaaagatg aaaaagacac ctgttgcaac caggaaacca
      961 aagaggttgt ccactgttgt taaagcggaa ttgaggcggg atgcgcttat cgcagacttg
     1021 atcgagactc gcagaaaaat cgttgatgtg gcttcgtctt tgtcaccgac cgagcaagat
     1081 caagtctttt tgggcatttg gtccatgaaa gatttgttgg cccacttgat aggatggctt
     1141 gaagaaaatc gcaaggcggt gaaaacgatc cgcgcgggca agctccccga attctatgcg
     1201 tataaagatc gcgattggca aacttataat gcgtatctcg tctcgaaata taaacgtgga
     1261 gacatgacag agttattggc gaagttgcag gtttcccagc agaaattcat aaaatcaatt
     1321 cgggccgttc ctattgaggt acttgaacag gattttgggg taaggtttaa tcggtataaa
     1381 gtcacaattg tccgcttgtt gcaggccgaa ctcaaggacg aaaaggtaca ttacgctcaa
     1441 atggaagcgt ttaggaataa ggcgaagtaa aattaacagc ggacgcaatc gaaacttgaa
     1501 gggtatgatt gctgttctca atcgcagaga gagtacatcc ccaagaggcg tttatgcaaa
     1561 acgaaggtga aagcaggtaa tggagatata tattcgtaaa gcaactgcaa atgattatga
     1621 cgatttatgt gaactcttcg atgaagtgga tgctttgcat cgcgttaatc tgccccatgt
     1681 ttttcaaaag tcgactggcc ctgctcgaga aaaagattat tacctggggc tgattgccga
     1741 tgagaacatt gggctttttg ttgcggaaat ggaagagaaa cttgcagggt ttgtgcatgt
     1801 gcttgtccga gatacccctg tcttttccat cgtgattccc cgccgttatg caatcgtcga
     1861 cgggatcgcc gtaaaatctg aatttcagaa gcatggaatt gggaaaatgc tgatggacaa
     1921 agcgcaggaa tgggcagaga ccaaagacgc ggaattcatg gaactgaatg tctacgagtt
     1981 taacaggaac gcgatctcct tttacgaagg tctcggctat caaagttcca gcaggaaaat
     2041 gagaaaggaa atcaacaagg cagggtgaaa aactgtagcc cggactggtt aagtgcaaca
     2101 ctgaaattcg aattgaatgg aaaaatcaag atcaggtttt gaaaattatc ggtcgtcctt
     2161 cttaatctta ctttggagag aataaaatgc ctgaaaacgt atcaacctac cagatcgtgg
     2221 atctgatgcc tgacttctgg gaattccatg caaaaaacag ggatgcagat gtttcggaac
     2281 aggcatccca atttaaaaga atggttgtgg ataaacaccc cgaagcctac gccgccaggg
     2341 tcattggcct tgatccaaac aaaccctatg acgaagcact tgccgagcgt tacctgcttt
     2401 gccatgagcg cagcacggct caacaagtca gcaccatgca gaggatatcc gcttcgatcg
     2461 cacgtgacat gtccgcgtat gaagaaaagt ttcgcaaggt ttttcccgac ttcaactata
     2521 caggttcggt ttacttcatg aattcgctcc tcggttttga tggggccacg cgtacggtca
     2581 atggaaagat cgccctgtta tttggtttgg atacgatggc atgggtgtac ggcgatgatc
     2641 tggacctgca gccatttttc catcatgaga tttttcacat ctaccattct caatttctgg
     2701 aagatgatag cgatgaagtc gccgccgccg tctggcagga ggggctcgct caatttgtgg
     2761 cgaagtcgtt gaatccagat gccgagggcg tgaatttgtt tgggcttcca aaggaaatgc
     2821 cgcagcgctc tgaagcaatg ctctccacac ttgccagtgg tcttcttagt gtgcttgatt
     2881 caaccagcag agatgattac ggcagatact tttttggaaa caatggcagt gaagagattc
     2941 caccgcgtag tggttactat ctgagttatc tggttgtgca gaaactttcg aaaaaatact
     3001 ccctgcagga aatggctcat tcgaaactgg cagccatcaa acccgaaatc aaaacggctc
     3061 tggaagaatt ggccaacggc ggaagatgac catgcaagga gaaacaatgg aagcctacga
     3121 tgacgatctc ggcagatttg aagcggaagg ggccgcacca ctgccagatt ctggaaatca
     3181 gggatatatc gatcatgaca acgcccgcat ctggtacgca gactatgggg tgggtccaac
     3241 cgtggtgttg ttgcacggcg gccttggcca tagcgggaat tggggatatc aaatccccac
     3301 cctgatcggg aatggctacc gcaccattgt catagacagc cgcggacatg gccgcagtac
     3361 gcgtgatgac cgtccatata cctatgagtt gatggcctcc gatgttttgg ccgtgatgga
     3421 tgttttagga gttgaaaaag cggcgctggc tggttggagt gatggcgcat gtatcgcgtt
     3481 ggtccttgct gcacaggtgc ctgcacgggt ggaaggtgtc ttcttctttg cttgcaatat
     3541 ggatccgagc ggcgcgaagg aaattaccga gttcgccccg attctccagc ggtgtatcgg
     3601 cagacatatc aaagactatg gtcaactgtc agaaacgcca gaccagtttg aggcgttgtc
     3661 tgaagcggtg ggtctcatgc agaggacaca gcccaattac tccgccaatg acctcgcgca
     3721 gatcactgtg ccggttgcgg tcatccacag tgagtttgac gaattcatca aacttgaaca
     3781 tgctgaatat cttgcacgca gcattccaaa cgcggacttt gtttatcttt cgggtgtgag
     3841 ccactttgcc ccattgcagc ggcctgacca attcaacgac acgcttctca atttcctcgg
     3901 caaagtgctg gtgtgaggcg cctgaaaagt tttagaccga tcttgtttta acaatatggt
     3961 cgtcatggtc gtttgggggt gcatctcaaa ccttgaaaac gcatagtaaa atccacgcac
     4021 aattgttttg cataaaaccg tcgtgcgtgg attttggttt tcacggcgga attttttgaa
     4081 ggatcgttat gactgataaa cccagcacaa cccagccgat catggataga cgccttcttg
     4141 tcttgcttgt tctatccgtt gcgtttcttg tcattgcctt tcttggcggc cagccttcat
     4201 cagcacgcgg tttctattat gccgtttggc tgacattctc aggttggcta tggaagaagc
     4261 gagagcgcct gagcctctgg ctgcaagccc gtccattgcc tgaatttccg ctttttgttg
     4321 ggctgggtct cgtcatgatc cttgttgaag aaaccattgc tggggtggct gtccacttgc
     4381 tgaatgtgga cagtgtaggg cagttgtttg ccgttgtgcc tttgtattat ttcaacaacc
     4441 tgcttttgct gcctggcttc atcgtcggct ggtatgttct gctcaagttt tgtgcatact
     4501 cgcggactga ggtgttcgtc ctcgtgggac tctttgggtt gttttcggaa aagatctata
     4561 tccatgtcct gacgatcccc attcttggga ttcccctgat ccttcctaca atgttcacct
     4621 acatggcgat catcatgccg tcgatccttt cattaagatc gcaaagcacc cgtccgttga
     4681 acaggccgct tcgatatctg ctggggttcg tgtttccgat catcgtttca atgcctttta
     4741 ttttcatcca cactttattg acgaatgcgg gtttaattga cccgaccatt ttgacaaaat
     4801 aaacctgttc tcgttgtgac agcccaccgt cattccccgt ctgagatgat ttatcgttgg
     4861 aggaacctgt gatgaaaaaa ctgttcatgc ttgggttggt tgttctgctt ttgctttcaa
     4921 cggctgttcc tgttctggca gggaccaatg cgtctcacgc tgctgtgccg aatcagtttg
     4981 tggatgttcc gcagaaccat tgggcggtgg cgtggatcaa ccagctttac accgaaggtg
     5041 taacgggcgg atgcagcact gctcccttgc gatattgccc cgatgcggtg gtcactcgcg
     5101 cgcaaatggc ggtctttctt gtgcgtgtca ttcatggcag cacattcaca ccgccagcca
     5161 cttcggtgat ctttgaagat gtcccagccg attactgggc cagtgcgtgg atcaatcagt
     5221 tgaacgcgga tggcgtcaca ggcggatgca gcagcgcccc gaaactattt tgtccagaag
     5281 caaatgtcag ccgcgcacag atggcggttt tcctcctccg cgccaaatat ggaagtgcct
     5341 atagtccgcc tgcctcggcg gatgtcttca gcgatgtgcc cgcggattat tgggcccgtg
     5401 attggatcgg acaactctac gccgaaggct acaccggggg ctgttcatcc aaccctttgc
     5461 agttctgccc cgaagcattt gtcagccgcg atcagatgtc tgtttttatc ctgcgtgcca
     5521 ttcacggcgc taattatgtt cccccgccca ttgaccagac ctccctgcgc ccgtttccgc
     5581 agcatgtcca gtatgcgccc ggcagcattc ttcccgatca tcgcacccag cagcagcttg
     5641 atgacgatgt ccgcgctttt tatgattact ggaaggcgaa ttacctgatc gcagcgggcg
     5701 tgaatgccga tggcattccg atgtttcgcg tggcgttcgg gaaaaatgaa cctgaccgtt
     5761 cagccaccgt atctgaaggg cagggctatg gcatgttgat tgtccccatc atggcggggt
     5821 atgaccctga tgcgcaagcc atctttgatg gattgtgggc cttcgcgcgt gcgcatccca
     5881 gtgaaggcga tccgcgcctg atggattgga aggtgcctca aaccgatggc aacgactcag
     5941 cctttgacgg cgatgcagat attgccttcg gtttactgct ggcagatgcc caatggggca
     6001 gcgcagggct cgtaaattac aaagccgaag cggaaacaat gatcgcaggt attttagaat
     6061 cgaccatcgg gccttccagt catctgccca tgctcggcga ctggacagat ccaaatggtg
     6121 tgacctacaa ccaatacacg ccgcgttcct ccgatttcat gctgaccaat ttccgcgcgt
     6181 atgggcaggc tacggggaat ccagtttggg atgatgtggt tgcacaaagc cagggtgtgg
     6241 ttgttgcaat gcaggccaat cacagtccgc aaacaggtct gctgcctgat tttattgtca
     6301 ggaaaaatgg cagttatgaa cccgctcccg tcaattttct ggaagcagat accgacggcg
     6361 cctacagcta caatgctggg cgcgacccgt ggcgtttggg tatggatgcc ttgttgagcg
     6421 atgatgccgc ctcacgtgca agtgcgttga agatctctca ttgggctgaa tctgcggtgt
     6481 ctggcagccc tgcaaacttc cgcgctggat ataacttgaa cggtacgcct ttgccaaaca
     6541 gcgattactt cacgtcgttt ttcgtggccc cgttgggcgt tgcggccatg aacgattccg
     6601 cccaacagga ttggctgaac gccgtttatg attcagtcta tgccactcgt gaagattatt
     6661 attcagacag cgtcactctg ctctgcctgc tggtgatgac aggaaacttt tggacaccat
     6721 gaactcaatc cactctcaat ccctcgtctg ggatgcacat gcctgcttcc cgctcaacgc
     6781 caactccgat ctgagcgaat tgaaacgcta caaagatagc ggcgtgaatt tcgtctccct
     6841 gaatatcggt atggatatgg attccttcga gaatgtgatt caggtgctgg cctgtttcag
     6901 gaaatatatt gccgcgtatc ctgacgacct cgtgcttgct cttgcggtgg acgatgtgat
     6961 cggcgcgaaa gcatctggca aactcgcagt ggccttcgac ctcgaaggct ccgacccctt
     7021 gcgcggagat ttgaatctca tttcgttcta ctatgacctt ggcgtccgtc aaatgctttt
     7081 ggcttataac aaagacaacc gcgcctcagg cggctgcatg gaaggtcaca tcggcctgac
     7141 cgattttgga aaacaggtga ttcgtgagat gaaccgtgtg ggcatggttg tggatgtgac
     7201 tcacatgggc tatcgcgcca cgatggaggc ctttgaaacc tctgcggcgc ctgtcatctt
     7261 ttcccattcc aaccccaagt cgctgcgcga taatgcgcgg aatatcaccg atgaacagat
     7321 caaagcctgt gccaaaacag gcggcgtgat tgcgatcaat ggcattggtg attttctcgg
     7381 cggaaccacc agcgagatca tggtcaagca catcgaata
//
LOCUS       DAOQJC010000165         7013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_5966_length_7013_cov_8.690428, whole genome shotgun sequence.
ACCESSION   DAOQJC010000165 DAOQJC010000000
VERSION     DAOQJC010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5966_length_7013_cov_8.690428"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1648)
                     /locus_tag="PLE39_17395"
     CDS             complement(<1..1648)
                     /locus_tag="PLE39_17395"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNE70162.1"
                     /translation="MKKARYPLGTPRYLLWVYLLILTIVFSACGSPTPITVVVTATSL
                     PPTEGVVEPTATLVPVNLSGPQSGDKIRWIDGSNLIYVPPGEFVMGNGGFDAPVHNVT
                     LDGYWMYQTKVTNRMFAQCVAVGSCTPPTEELGGPVYSNPEYANHPVVGVTWDQAQAY
                     CGWANGQLPTEAQWEKAARGLAGNLYPWGNDEPACDLVNFGYCLGRTTEVDAFPDGAS
                     DFGMYDMAGNLFEWVSDWYSETYYSEAPAANPLGPDSGQYRVIRGSSFETDLELVESA
                     IRHFGAQGYHTRDIGFRCTVPQPQLFAPYCQLSAYIPSGVIVSNGCQLPEAAVEGQYC
                     AVGRSFATVSLSPGAIYQTGEGLECEEAIVDGQRILTCVGPFAQEATNEITVCNPTCS
                     NSPDVTGATPTCDPGYTLDPTTNTCNYSPVPSELSVAGCPVGYNLVDRGGQQTCVIAT
                     NANGECPNGLYYDDLAGRCVPPNGLVEAPIGINNAPLAAQSYSGCAAGFTYSDTFQCC
                     QAVTGGTYPGCSPGSTFNRDLGACSPGEIRLAGPGCVTLDV"
     gene            complement(1710..>3242)
                     /locus_tag="PLE39_17400"
     CDS             complement(1710..>3242)
                     /locus_tag="PLE39_17400"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE70163.1"
                     /translation="VGLFMPMTPEIVIALLAIAKIGGVVLPLFSGYGAGAIVSRMTDA
                     NAKALFAADGAFRRGKAVEMKSIADEAAEQIGSLQHMIVLRRTGQAIQMKAERDHWWH
                     ELVDGQSDVCETERTSAEDPLMIIYTSGTTGRPKGAVHTHCGFPIKAAQDMAFGTDVH
                     GAFRAERSDVIYWMTDMGWMMGPWQVFGSLLLGATMFLYDGAPDFPAPNRLWELAEKH
                     RINQMGVSPTLIRSLIPQGDGHFEKHDLSSLKCFASTGEPWNPDPWMWLFEKVGGGRI
                     PIINYSGGTEISGGIVMGNLLLPLKPCAFSAACPGMDADVVDENGRSIRNAVGELVVK
                     APWIGMTRGFWNDRQRYLDTYWSRWENVWVHGDFAAIDGDGLWYILGRSDDTIKIAGK
                     RLGPAEVESILVRHGSVVEAAAIGVPHEVKGSELVLFAVTGPGVVGGDALRQELYEMV
                     VAEMGKPLAPKAILFVSDLPKTRNAKVMRRVIRSAYLGLELGDTSSLVNPQAVEEIRR
                     AK"
     assembly_gap    3243..3342
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3343..3554)
                     /locus_tag="PLE39_17405"
     CDS             complement(<3343..3554)
                     /locus_tag="PLE39_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70164.1"
                     /translation="MTEQFTFGGEIVWKPSREQIEHANLTAFMKQHGIKDFNELMKRS
                     TEDVAWFTDAVLKFLDIQFYEPYSQVV"
     gene            3708..4070
                     /locus_tag="PLE39_17410"
     CDS             3708..4070
                     /locus_tag="PLE39_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70165.1"
                     /translation="MTQNTFPCIKCGGPNEPEAGQTSMACIYCGAALTIPEELRVKSM
                     PRVEKIQPKPKPAPSLEKETPELLRKAQPLAIKAWNMYAYWTWIRWVLPTCLTVLAVA
                     FFICMALGALPFVFNWFR"
     gene            complement(4041..4481)
                     /locus_tag="PLE39_17415"
     CDS             complement(4041..4481)
                     /locus_tag="PLE39_17415"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HNE70166.1"
                     /translation="MNGNDFMAWVLRSPLHGMLSGGMMLMTVTGRKTGKKYTTPVGYY
                     VEGEHLWVITSRERTWWKNLRGGATVDLLLKRKPVQGMADVELDEKSVAARMNEYLHH
                     VPQAARPMGIRVENSKPNAEDVARTAKDRLFVRIKLAEPIKNER"
     gene            complement(4522..5490)
                     /locus_tag="PLE39_17420"
     CDS             complement(4522..5490)
                     /locus_tag="PLE39_17420"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HNE70167.1"
                     /translation="MKVLVVGAGIIGTIFGWALAEDGHDVTHFVRKGKAGNFPHGAQI
                     DMLENRKGKKNFIGRYDIKLAEVLSPSDGFEAVIVPTKPYQLMDVLKEIVPQTGNADY
                     LLLTQNWTGTAEIDAVLPQARYIFGDAHAGGVWKDGVLIATIMDDIILGQVDRGRGAV
                     LQKFADLFTGINLKVTIPENILHYIWVQHAMTTGLWMGLVRTGSLENILKDPVNGPLS
                     LQAVKECLTIAEKRGIDLQKYPEIKMYLQTDSKIGMAITGFVVRMMFKLNKSIQRSSA
                     HGLGDPIEIRSAYEDLLKSGAEFGVPMPIMQSFRPDVERLVAIGVH"
     gene            complement(5503..6195)
                     /locus_tag="PLE39_17425"
     CDS             complement(5503..6195)
                     /locus_tag="PLE39_17425"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE70168.1"
                     /translation="MTQTRREKLRDATREEILSTAWKQIAEAGASGLSLRGIAREMGM
                     TAPGLYRYYKDRDALVTVLLMDAFGSFADALETGRDACAADDHAGRFREMGRAYFRWG
                     SENPQKYALLFGSPIHGYVFAEELVPVAQKSFFVLVGVIGAAFAAGKINGEIPALKLP
                     TSLKVQYDALKKMGIPFSGGVIQLALSAWAQMHGITSLYLQNYLSGMLQDGVGAFVDF
                     EIEKMIKIFGME"
     gene            6331..6957
                     /gene="upp"
                     /locus_tag="PLE39_17430"
     CDS             6331..6957
                     /gene="upp"
                     /locus_tag="PLE39_17430"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNE70169.1"
                     /translation="MSNVYASKHPLIAHKLSRLRDKKTEPKKFRELVREIAGLLAYEA
                     TADLATTPIEIETPLETMTAHQLKEKIGLIPILRAGLGMVEGIWELMPSAEVWHIGLY
                     RDEHTLQPVEYYNKLPVDPRVSVCLILDPMLATGGSATATADILKRWGVTKIKFVGLI
                     AAPEGIKAMQEAHPDIDIFIAAIDDHLNERAYIVPGLGDAGDRQFGTG"
BASE COUNT         1700 a         2079 c         1559 g         1575 t
ORIGIN      
        1 tcacatccag tgtgacacag cccgggccgg caagcctgat ctcgcctggc gaacaggctc
       61 cgaggtcccg attgaacgtg gagccaggag agcagcctgg gtaggttcca ccggtcacag
      121 cctgacagca ctggaacgtg tcactgtagg taaatccagc cgcacagccc gaatatgatt
      181 gagccgccaa cggggcgtta ttgataccaa tcggcgcttc caccaaccca tttggcggaa
      241 cacatcgccc ggcgaggtca tcgtaataca aaccattcgg acattcaccg ttggcatttg
      301 ttgcgatcac acaagtctgc tgaccgccgc gatccaccag attgtagccg acggggcatc
      361 ccgccacact taattcactg ggaaccgggg aatagttaca agtattggtg gttgggtcga
      421 gggtataacc agggtcacaa gttggcgtgg ccccagtgac atctggagag ttgctgcagg
      481 tggggttaca aaccgtgatc tcattggtcg cttcctgtgc aaacggcccc acacaggtca
      541 aaatacgttg accatccacg atggcctctt cgcattccaa accttcgccc gtctggtaaa
      601 tggcgccagg tgaaaggctc actgtggcaa aactacgacc tactgcacag tactgaccct
      661 ccaccgctgc ttcaggtaac tgacacccgt tcgaaacgat cacccccgag ggaatgtacg
      721 ccgaaagctg acagtatgga gcaaacaact gcggctgcgg aactgtgcaa cgaaaaccaa
      781 tatcgcgagt gtgatagccc tgagcgccga aatgccgaat cgcagactcg accagttcaa
      841 ggtctgtttc gaacgaacta ccacgaatca cgcgatactg gccgctgtca ggtccaagcg
      901 gatttgcggc gggtgcttcg ctgtagtagg tctcactata ccaatcgctc acccactcga
      961 agagattccc cgccatgtca tacattccaa aatcactggc accgtcaggg aacgcatcca
     1021 cttcggtggt gcggcccaaa caatagccaa aattcacaag gtcacaggcg ggttcgtcat
     1081 tgccccacgg atataaatta ccagcaaggc cgcgggccgc tttttcccat tgtgcctcgg
     1141 tcggcaactg gccattggcc cagccgcaat aagcctgcgc ttgatcccag gtcacaccca
     1201 cgacagggtg attggcatat tcggggttgc tatacacagg gccacccaat tcctcggtgg
     1261 gcggtgtaca agaaccaacc gccacacact gcgcgaacat acgattggtc accttggtct
     1321 gatacatcca gtacccatcc agggtcacat tgtgaaccgg ggcatcgaaa ccaccgttcc
     1381 ccatcacaaa ttcaccgggc ggcacataga tcaggttgct tccatcaatc cagcgtattt
     1441 tgtcgccgct ttgcgggccg gaaaggttca caggcactaa agttgcggtc ggctcaacca
     1501 ctccctcggt cggaggcagg gaggtagccg tcaccaccac cgtgatcggc gttggagatc
     1561 cgcaggccga aaacacaatg gtcaggatca aaagatatac ccagagcaaa taacgcggtg
     1621 tacccaatgg gtaacgagct tttttcatta aatctccttc tcccgcaagt tctgcggggc
     1681 gcccgttatt ataatgcagg gtcaaaaaat cattttgcgc gccgaatctc ctccaccgcc
     1741 tgtggattga ccagtgacga ggtatctccc aactccaaac caaggtacgc cgaccgaatc
     1801 acccgccgca tcaccttggc attccgagtt ttgggcaggt cagaaacaaa gaggatggcc
     1861 ttcggcgcca gcggcttgcc catttcagcg accaccatct catataattc ctgacgaaga
     1921 gcatcgcccc caacgacccc ggggccggtc actgcgaaaa ggaccaattc actccccttg
     1981 acctcgtgcg gcaccccgat cgccgccgcc tccaccactg aaccatgcct cacaaggatc
     2041 gactccacct cggctggccc cagccgcttc cccgcgatct tgatcgtatc gtccgagcgc
     2101 ccgaggatgt accaaagccc gtcgccatca atcgcggcaa aatctccatg gacccacaca
     2161 ttttcccagc gcgaccagta ggtatcgaga tagcgctgcc tgtcattcca aaaaccacgc
     2221 gtcatgccga tccacggcgc tttgacgacc agctctccca ctgcattgcg aatggacctt
     2281 ccattttcat ccaccacatc cgcatccata ccggggcagg ccgccgaaaa cgcacacggt
     2341 tttaaaggca aaagcagatt ccccatcacg attccgcctg agatctcggt gccgcccgaa
     2401 taattaatga tcgggatgcg cccgcctccc accttctcaa acagccacat ccacgggtct
     2461 ggattccacg gctcgcctgt ggaagcaaag cacttcaagg atgaaagatc gtgcttttca
     2521 aaatgcccat ctccctgcgg gatcaaagag cggatcaaag tgggcgaaac tcccatttgg
     2581 ttgatgcgat gcttttcagc cagttcccac aagcggttcg gggcagggaa atcaggtgcg
     2641 ccatcataaa ggaacatcgt cgcccccaac agcagacttc caaacacctg ccacggcccc
     2701 atcatccaac ccatatcggt catccagtag atcacatcgg agcgttcagc acgaaatgcg
     2761 ccatgcacat ccgtcccaaa agccatatcc tgcgcggctt tgatcggaaa tccacaatgg
     2821 gtatgaacag cgccctttgg cctgcccgtc gtccctgaag tgtaaatgat catcagcgga
     2881 tcttcagcag atgtccgttc agtttcgcaa acgtcacttt gtccatccac caactcatgc
     2941 caccagtgat ctctttcggc tttcatctgt attgcctgac ccgtccgcct caatacgatc
     3001 atatgctgca gtgagccgat ttgttccgcc gcctcatccg caatggactt catttccaca
     3061 gccttgccgc gtcgaaaagc gccatctgcc gcaaaaagcg ccttcgcatt tgcatctgtc
     3121 atgcgtgaga cgatggctcc tgctccataa cccgaaaaca acggcaggac aacgcctccg
     3181 atctttgcaa tggcaagcag agcaatgaca atttcaggag tcatcggcat gaacagccca
     3241 acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaccacttg agaatatggt
     3361 tcgtaaaatt gaatatcaag aaacttcagc actgcatcgg tgaaccaggc gacatcctcc
     3421 gttgaccttt tcattaattc gttgaagtct ttaattccat gctgtttcat gaaagcggtc
     3481 aggtttgcat gttcgatctg ttcacgggat ggcttccaaa cgatctcacc gccgaatgtg
     3541 aattgctcgg tcatggatat tctcctctgg ttggggttgt gctgattcta tcgcaacttg
     3601 cttttatacc gcattcggtc acaaattgcg ctttttgaga aaagagagga gcgcaatttg
     3661 tgaccgtggg tattatataa ttgccccgat ctggagaacc taattcaatg acacaaaaca
     3721 catttccctg cattaaatgc ggcgggccaa acgagcccga agcaggacag accagcatgg
     3781 cttgcatcta ttgcggcgcc gcgcttacca tccccgaaga gctgcgggta aagtccatgc
     3841 cccgggtgga gaaaattcag ccgaagccaa aaccagctcc cagtttggaa aaagaaacgc
     3901 ccgaacttct tcgcaaggca caaccccttg ccatcaaagc ctggaatatg tacgcctact
     3961 ggacgtggat ccgctgggtg cttcccacct gtcttacggt tcttgccgtc gcttttttca
     4021 tttgcatggc gttgggtgca ttaccgttcg tttttaattg gttccgctag tttaattctt
     4081 acgaacaatc tatcttttgc tgtacgggca acatcttctg cattcggttt gctattttcg
     4141 acgcggatgc ccattggcct ggccgcctgc ggaacatgat gcagatactc gttcatccgc
     4201 gccgctacag atttttcatc gagttcaaca tcggccatgc cctgcacagg ctttcgcttc
     4261 aacaacaggt caaccgttgc gccgcctcgc agattcttcc accacgtccg ctctcggctg
     4321 gtaatgaccc acagatgttc gccttccaca taatagccaa caggggttgt atatttttta
     4381 ccagtcttgc gccctgtaac ggtcatgagc atcatgccgc cacttaacat gccatgcaac
     4441 ggcgaacgca acacccaggc cataaaatca tttccgttca ttcataactc cttcctgcca
     4501 tttcaaccgc accgcggaaa actaatgtac cccaatcgca acaagtcttt ccacatcagg
     4561 gcggaagctc tgcatgatcg gcatcggcac accaaattcc gcacccgatt tcagcaggtc
     4621 ttcatatgcc gagcggatct caatgggatc acccaaacca tgtgccgacg aacgctgaat
     4681 gcttttattg agcttgaaca tcatccgaac cacaaaaccc gtaatggcca tgccgatctt
     4741 tgagtcggtc tgcaaataca ttttgatttc gggatatttt tgcaggtcga tgccgcgctt
     4801 ttcggcaatg gtcaaacatt ccttcacagc ctgcaaactc aacggcccat tgacggggtc
     4861 cttcaaaata ttttccaaac tacccgtgcg gaccaacccc atccacaagc ctgtggtcat
     4921 ggcatgctgt acccagatgt aatgcaaaat gttttccggg atggtcactt tcaaattgat
     4981 tccagtgaat agatcggcga acttttgcaa taccgcacca cgcccacgat ccacctgtcc
     5041 caaaataata tcgtccataa tggtggcgat caaaacgccg tctttccata ccccacctgc
     5101 atgtgcatcg ccaaaaatat atcttgcttg gggaagcact gcatcgatct ctgccgtacc
     5161 ggtccagttc tgggtcagca acaggtaatc tgcattccca gtctgtggca cgatctcctt
     5221 caaaacatcc atcaattgat acggcttggt cggtacgatc actgcctcaa atccgtctga
     5281 aggagacaaa acctcagcaa gtttgatgtc atacctgcca atgaaattct ttttgccttt
     5341 gcgattttca agcatatcaa tctgcgcacc atgcggaaaa ttccccgcct tgcctttgcg
     5401 aacaaaatgc gtcacgtcat gcccatcctc cgccagtgcc cacccaaaga tcgttccaat
     5461 gattcccgct cccacaacca acactttcat gccttctcct ttttattcca ttccaaaaat
     5521 cttgatcatt ttttcgatct caaaatcaac gaacgcgccc acgccatcct gaagcatccc
     5581 agaaagatag ttttgcaaat acaacgacgt gataccgtgc atctgcgccc aggcagataa
     5641 agccaactgg atgacgccgc ctgaaaacgg gattcccatc ttcttcaacg cgtcatactg
     5701 gaccttgaga ctggtcggca gtttaagggc tggtatctcc ccgttgatct tccctgctgc
     5761 aaacgccgca ccaatgacgc ccaccagcac aaaaaaactt ttctgcgcca ctggcaccaa
     5821 ttcctctgca aaaacatacc catggatcgg gctgccaaaa agcaatgcat acttctgagg
     5881 attttcactc ccccagcgaa aatatgccct gcccatttca cggaagcgtc cggcgtgatc
     5941 atcggcggca caggcatcac ggccagtctc aagcgcatcg gcaaacgaac caaatgcatc
     6001 catcaacaac actgtgacca gtgcatcacg gtctttgtag tagcgataca gccccggcgc
     6061 cgtcatcccc atttcgcggg cgatcccgcg cagggaaagt cccgaagcgc ctgcttcagc
     6121 gatctgtttc catgcagtgg agagaatctc ctcacgggtt gcatcgcgta atttttccct
     6181 tcgtgtttgt gtcatggctg cctttttgtt acatgtgatt atttttgttt acagcgttta
     6241 ctattttagc aaaaatctca cccctgtcaa gagcaaacag gctgcctttc tcacgctata
     6301 attgcgccaa ctcactccaa ggagaatgtc atgtcaaacg tgtatgcgtc caaacaccca
     6361 ctgatcgccc acaaactttc acgcttgaga gataaaaaaa ccgaacccaa gaagttccgc
     6421 gaactcgtta gagagattgc gggacttctt gcatatgaag ccactgccga cctggccact
     6481 acacccatcg agatcgaaac cccgctcgaa acaatgacag cccatcagtt aaaggaaaag
     6541 atcggtctta ttcccatttt gcgcgccggt cttggcatgg tggaagggat ttgggagctc
     6601 atgcccagcg ccgaggtctg gcatatcggc ttgtatcgtg acgaacatac cttgcagccc
     6661 gttgagtatt acaacaaact tcccgtcgat ccgcgtgttt cggtctgtct tattcttgac
     6721 cccatgctcg caacaggcgg ttcagccaca gccacagccg atatcctcaa acgctggggc
     6781 gtgaccaaga tcaaattcgt cggcctgatc gccgcccccg aaggaatcaa agccatgcaa
     6841 gaggcacatc ctgacattga tattttcatt gccgccattg acgatcacct gaacgaacga
     6901 gcttacattg tgcccgggct tggagatgca ggtgaccgcc agtttggcac gggataaata
     6961 gaactcttgc ataagtccat tttttaccac aacacttgcg ccgcacgcta gtg
//
LOCUS       DAOQJC010000166         6991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_5994_length_6991_cov_9.658737, whole genome shotgun sequence.
ACCESSION   DAOQJC010000166 DAOQJC010000000
VERSION     DAOQJC010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5994_length_6991_cov_9.658737"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PLE39_17435"
     CDS             complement(<1..870)
                     /locus_tag="PLE39_17435"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNE70170.1"
                     /translation="MKKYWPTTRDYLLIIASSLIQAVSLRLFFIPANLASGGVSGISQ
                     LINHYTGWPIGLMVLIGNIPLFLLGWRFLGGVKFAMRTAVAILTYSLFTDLILKVPYF
                     VEYSQILISDLSGDIFLNSLYGAIISGIGYGMVYRARGTSGGSDILARILNNWRGIPM
                     TQSYLIVDTAVILSAGFVFGWKEALYAMITLYVSGIVSETVLEGGGTVRTAMIVTAKP
                     EEVSNRVIEELERGVTWLEGRGAYTGNSRPVLYCVVNRAEIASLKAIVHESDPEAFMV
                     IGVAHEALGEGFKP"
     gene            complement(907..3249)
                     /locus_tag="PLE39_17440"
     CDS             complement(907..3249)
                     /locus_tag="PLE39_17440"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNE70171.1"
                     /translation="MKLPALRMQDLTFGLSLLALICLPIALSEIVRDAGVSLLLPLTL
                     IGAVLAWALAGWGVRKLFSGFILLLLGPLVLYVRIGQLWDSLFEWVRQFFALLPNFFD
                     AAINDTQLDSTLLAAAQDVFTQRALAFSGRITLWVTAMARGVQIEDPVVRTLIWCLAI
                     WLIAVWAGWQIYRNQKFTLGMFPSTVVLAFVLDYTGKEKEILWFHLALLLFLYGLTNY
                     QSLQKRWDARKIDYSESTSIDTLAATGALTVVLVAVAFLVSTFSIKDILDDFRERRAG
                     SNESQAESLGLESVKDNFRVTGFGNGLPRSYLLKAGPEISTQLAMTISTGELPPMSRN
                     AHPNVPRYYWRTLTYSIYTGAGWSNPTIGAEDIPSNQPLIETENANQRIVRAQITYPD
                     DFSERLYWAGTFISTDVPIQAAWVHRSQDANPLDTDLLAAFAPVETYTVESAILNVSV
                     QELRDSPSVYPDWVRKQFLGLPDSVPERVLSLARDLTASEANAYDRAIAIQNYLREFP
                     YTLDISSPPAGRDVTDYFLFDLKQGYCDYYATSMAVLARAAGLPARLVVGYANGNYDL
                     ERARYIVTENYAHSWVEIYFAGIGWVEFEPTASEPVILREDQNDAAASTADSQPEEDQ
                     AQNKFTLPLQNIWASIFALLALASIGIGIDTLRLKRIPPAQTIQILYKRLRRLARPVT
                     GYASKNRTAYLYSSDLIRSLSALDASPRLQNWLAPARNEIELLTELFSRSLFAPTEPS
                     RAEAEQAIQAWASLQWRLALANLLIVVARHNMSRYQPPRL"
     gene            complement(3246..4520)
                     /locus_tag="PLE39_17445"
     CDS             complement(3246..4520)
                     /locus_tag="PLE39_17445"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNE70172.1"
                     /translation="MKLNSVLPPLLSLTMLVMQILDPSKIWQALFVAFSGLWLAAWWW
                     ARSLKLNIRLTREVRFTWAQVGDALEERFTLTNHGHVPATWVEVVDHSTLPDYSAACA
                     VGVDQASVNVWSTRAVCKRRGVYELGGTTLRCGDPFGIYSVEIFLPEKTTLVVMPPVI
                     PLPTVEITPGGWMGDGRPRPNLVDQTVNAATVREFTQGENPKIIHWPTTARQRKFYAR
                     QLDGAPASDWWIALDADAGVQAGADWENTLELGIILAASLADRSLRARHSVGLLASGR
                     QTTWLKPQSGERHRLEIFRALAMLEPGQLPLAQLLERANSSLGNRISLIVITPSIKTD
                     WIPALTHLMWKGIHPTVLLMDPSSFGAPQKADALAGIFAQLGIPRFILNRSLLQQPEA
                     HPGEGQWAWRITPSGKAISARPPSDMTWKRLG"
     gene            4601..5776
                     /locus_tag="PLE39_17450"
     CDS             4601..5776
                     /locus_tag="PLE39_17450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNE70173.1"
                     /translation="MSITLNPNREKSLLRRHPWIFASAIRHVDETHASGSTVDLLSSE
                     GQFLARASYSPNSQIRARVWTFNDEPVDEEFFRKRIRAAIEMRSVLSGSSKSLRLIHA
                     ESDGLPGLIVDRYEDVLVLQSLTAGSEFWKETIADLLVEETGIATIYERSDADVRELE
                     GLKPITGILRGTITNLQLPITEYGLEFKVDIAHGHKTGFYLDQRENRRRVGELSKNRD
                     VLNCFCYTGGFSIHALANGAKSVLSVDSSADALALLEENIALNQLPADRHTSLEGDVF
                     QLLRKFRDANRSFDLIVLDPPKFAPTAAHAEKASRAYKDINLLAFKLLRRGGLLFTYS
                     CSGGIDAALFQKIVASAALDAGVDATIIEHLSQGSDHPVSLHFPEGMYLKGLVCVKG"
     gene            complement(5803..6927)
                     /locus_tag="PLE39_17455"
     CDS             complement(5803..6927)
                     /locus_tag="PLE39_17455"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNE70174.1"
                     /translation="MSGLNNAIQLIREGRKEEARKILELILRSEPGNIQAWFWSVETY
                     SSLEKRIQVLELCLEINPGNAQVLQALQKLRSQRPAQNAVTPPPAQPTHQTYSAPLRD
                     EPPKPAASSYEPMYYEDKPKASSSTHPYFDDEPSAETDHYAYTSTPQKKDTISYSDTF
                     SLTKPKPTRKSYAFHEVWMIALSMLRMDAYEDTLNDPEATTGRALEWIAYAGIISGLL
                     FPFTLGASPQFAELQTMSELNQFFERFGTVAFTLILALAMAVISPLLSVISLTISAWF
                     QNLIAAFMGGHGNFTRTIYAQAAYLAPLSILIAVIGIIPVVGQCFNSLVGIYGLVLNI
                     RALQAAHSLTTGKAIGVIFLPSIFMIGFLCLAFFIVSAFN"
BASE COUNT         1599 a         1840 c         2000 g         1552 t
ORIGIN      
        1 cggtttgaac ccttcgccca gggcctcatg tgcgaccccg atgaccataa atgcttcggg
       61 gtcgctttcg tgtacgatgg cttttagcga agcgatttca gcacggttga ccacgcaata
      121 caaaactgga cgtgaattac ccgtgtaggc gccgcggcct tcgagccagg tcacgccgcg
      181 ttcgagttcc tcgatcacac ggttggaaac ctcttcgggt ttggcagtga cgatcattgc
      241 cgtgcggacc gtgcctccgc cttcgagcac cgtttctgag acgatgccgc tgacgtacag
      301 cgtgatcatt gcgtacaacg cttccttcca tccgaagaca aagcccgcag aaaggatcac
      361 tgccgtatcc acgatgagat agctctgggt catggggatg ccgcgccagt tgttgaggat
      421 gcgcgcaagg atatccgagc cgccgctggt tccgcgtgcg cgatagacca tgccatagcc
      481 gatgccgctg atgatggcgc catacaatga gttgagaaag atatcgccgc tcaagtcgct
      541 gatgaggatc tgcgaatatt cgacaaaata ggggactttc aaaatcaggt cggtgaacag
      601 tgagtaggtg aggatcgcca ccgccgtgcg catggcgaac ttgaccccgc caaggaaacg
      661 ccagcccagt aagaacagcg gaatattgcc gatcaacacc atcaaaccga tgggccagcc
      721 tgtgtagtga ttgatgagtt gcgaaatgcc gctcacgccg cccgaggcca ggttggcagg
      781 gataaagaac aggcgcaaac tcaccgcctg tatcaggctg gatgcgatga tcaaaagata
      841 atcccgcgtg gtgggccagt attttttcat gaaacttcct tttttaaaac taaatttgtg
      901 gaggcattat agccgagggg gttggtaacg cgacatatta tgtcgcgcta ccacaattaa
      961 taaatttgcc aatgccaatc tccactgcaa actagcccag gcttgaatgg cttgctctgc
     1021 ttcggcgcgg gatggttccg tcggcgcgaa caggctgcgc gagaacaatt cggttaatag
     1081 ttcgatctcg ttgcgtgcgg gtgcaagcca gttttgcagg cggggtgaag catcaagtgc
     1141 cgataaactt cggatgagat cagaggaata caaatacgct gttctgtttt tcgaggcgta
     1201 ccctgtcaca ggtcgggcga ggcggcgcag gcgtttgtac aagatctgga tcgtctgtgc
     1261 gggcggaatc cgcttgaggc gcagggtgtc aattccgata ccgatgctgg caagtgcaag
     1321 caaggcaaag atggaagccc aaatgttttg cagcggcagt gtgaatttat tctgcgcctg
     1381 atcttcttca ggttgtgaat cggctgtaga ggcggctgca tcgttttggt cttcgcgcaa
     1441 aatgacgggt tcgctggcgg ttggttcgaa ttccacccag ccgatgcccg caaaatagat
     1501 ttccacccag gagtgggcgt aattttcagt gacgatgtat cgtgcgcgtt cgaggtcgta
     1561 gttgccgttg gcatagccca ccaccagccg tgcgggcagc cctgcggcac gcgccagcac
     1621 cgccatggaa gtagcataat aatcgcaata cccctgtttt agatcgaaga gaaaataatc
     1681 ggtcacatcg cggcctgcgg gcggcgaaga aatatcgagc gtgtacggaa attcgcgcag
     1741 ataattctgg atggcgatgg cgcggtcgta ggcattggct tcggatgcgg tgagatcgcg
     1801 cgccagagat aacacccgct caggcaccga atcaggcaga ccgagaaact gcttccgcac
     1861 ccaatcagga tacacgctgg gagaatcgcg caattcctgc acgctgacgt tgagaatcgc
     1921 cgattcaacg gtgtaggttt ccacgggtgc aaatgccgcg aggaggtctg tatcaagcgg
     1981 gttggcatcc tgcgaacgat gcacccatgc ggcttggatc ggcacatcgg tgctgatgaa
     2041 tgtccccgcc caatacaggc gttcgctgaa atcatcgggg taggtgattt gggcgcggac
     2101 gatgcgttgg tttgcgtttt cagtttcgat cagcggctgg ttcgacggga tgtcttctgc
     2161 gccgatcgtt gggttcgacc accctgcgcc cgtgtaaata gaataggtca aggtgcgcca
     2221 ataatagcgc ggcacatttg ggtgtgcatt gcgcgacatg ggcggcaatt cgcctgtgga
     2281 aatggtcatg gctagttggg tggagatctc tggccctgcc ttcagcaggt aggaacgcgg
     2341 caggccgttg ccgaatcctg taaccctgaa attatctttg accgattcca acccgagcga
     2401 ttcagcctgc gattcgttcg agcctgcacg cctttcacga aaatcatcga ggatgtcttt
     2461 gatcgaaaac gtggaaacga ggaaggcaac cgccaccaat acaacggtca gcgcgcctgt
     2521 ggcggcgagg gtgtcaatgc tggtgctttc ggaataatca attttgcggg catcccagcg
     2581 cttttgcagg gattgataat tggtcagccc gtacagaaat aaaagcagtg caaggtgaaa
     2641 ccagagaatc tccttttcct tgcccgtgta atcgaggacg aatgccagca ccacagtgga
     2701 tggaaacatg cccagtgtaa atttttgatt gcggtagatc tgccagcctg cccacactgc
     2761 gatcaaccag atggcgaggc accagatcaa ggtgcgcaca acggggtctt cgatctgcac
     2821 gccgcgcgcc atggccgtga cccacaatgt gatgcgtccg ctaaatgcca gcgccctttg
     2881 cgtaaaaaca tcttgtgcgg cggccagcag cgtggaatcg agttgggtgt cgtttatggc
     2941 tgcatcgaaa aagttgggga gcagggcgaa gaactgcctg acccattcaa acaatgaatc
     3001 ccacaactga ccgatgcgga cgtataaaac cagcggaccc agcaaaagca ggatgaaccc
     3061 tgaaaataat tttcgcacgc cccagccagc cagtgcccat gccagcaccg cgccgatcaa
     3121 ggtgagcggc agcaacaaac tcacacccgc atcgcggacg atctcgctta atgcgatcgg
     3181 caggcagatg agcgcgagca gtgaaagccc gaaggtcagg tcttgcattc gcaaggcggg
     3241 cagtttcatc cgagcctctt ccaggtcatg tcgctgggtg ggcgggcgga gatggctttg
     3301 cccgagggtg tgatgcgcca agcccactgc ccttcgccag gatgtgcttc gggttgttgc
     3361 agcaggctgc ggttaaggat gaagcgtgga attcccaatt gggcgaaaat gcctgcaagg
     3421 gcgtccgctt tttgaggggc gccgaatgac gaggggtcca ttaaaagcac ggttgggtga
     3481 atgcccttcc acattaaatg ggtgagtgcg ggaatccaat ccgtttttat ggaaggggtg
     3541 atgacaatga ggctgatgcg attgccgagc gatgagttgg cgcgttcgag caattgcgcc
     3601 agcggcagtt gaccgggctc aagcatggcc agcgcgcgga atatttccag ccgatgccgt
     3661 tcacccgatt gcggtttcag ccaggtggtt tgcctgccgc tcgcaagcag gcccaccgag
     3721 tgccgcgcgc gcagactgcg gtcagccaga gatgcggcca ggatgatgcc gagttcaagc
     3781 gtgttctccc aatcggcgcc tgcctgcaca cctgcatcgg catcgagtgc gatccaccaa
     3841 tcgctggcgg gtgcgccatc caattggcgc gcataaaatt ttctttgccg cgccgtggtg
     3901 ggccagtgga tgattttcgg gttttcgccc tgagtgaatt cgcgcacggt ggccgcattt
     3961 acggtttggt caacaaggtt tgggcgtggg cgtccatcgc ccatccaacc gcctggtgtg
     4021 atctccactg tgggcagggg aatcacaggc ggcatgacca ccagcgttgt tttttcaggc
     4081 agaaaaattt caaccgaata aattccgaaa gggtcgccgc agcgcagggt tgtgccgccc
     4141 aattcataca caccgcggcg tttgcaaaca gcccgcgtgc tccacacatt gacggatgcc
     4201 tgatccacgc caaccgcaca agccgccgaa taatcgggca gggttgaatg gtccacaact
     4261 tcaacccagg tggcgggcac atggccgtga ttggtcagtg taaaacgctc ttcgagtgca
     4321 tcgcccacct gcgcccatgt gaatcgaact tcgcgcgtca ggcgtatgtt cagtttcaaa
     4381 gaccgcgccc accaccatgc cgccagccac aatccgctga acgccacgaa caacgcctgc
     4441 cagatcttcg atgggtcgag gatctgcatc accagcatgg tgagcgataa cagcggcggc
     4501 aggacggaat ttaatttcat gcgcgcgatc atcttcccat tataagctca gctttttgca
     4561 ggggaggcgg atcgaaaaga ttttgcggta agataagcgc atgtccatca ctctcaaccc
     4621 aaaccgcgaa aaaagtttgc tgcgccgcca tccgtggatc tttgccagcg ccatccgtca
     4681 tgtggatgaa acccacgcat caggctcaac cgttgacctg ctctcgtccg aggggcagtt
     4741 ccttgcccgg gcatcctact ccccaaattc gcagatccgc gcccgtgtgt ggacatttaa
     4801 cgatgaaccc gtggatgaag agtttttccg caaaagaatc cgcgcggcca ttgaaatgcg
     4861 gtctgtgctg tcgggctcat caaaatccct gcggctgatc catgccgagt cagatggcct
     4921 ccccgggctg atcgttgacc gatatgagga cgtccttgtt ctgcaatcgc tcaccgctgg
     4981 ctccgaattc tggaaggaaa ccatcgcaga ccttctggtg gaagagacgg gcattgccac
     5041 gatctacgaa cgctccgatg ccgacgtgcg cgaactcgaa ggcttgaagc cgatcacggg
     5101 tattctgcgc ggcacaatta ccaatctcca attaccaatc accgaatacg gcctcgaatt
     5161 caaggttgac atcgcgcatg gccacaaaac gggtttctac ctcgatcagc gcgaaaaccg
     5221 ccgccgtgtg ggtgaattgt cgaaaaaccg cgatgtatta aattgcttct gctacacagg
     5281 cggattctcc atccacgctt tggcgaacgg ggcgaaatcg gttctgtccg tcgattcctc
     5341 tgcggatgcg ctggccctgc tcgaagaaaa tattgccttg aatcaactcc ccgctgaccg
     5401 ccacacttct cttgaaggcg atgttttcca actgctcaga aaattccgcg atgcgaatcg
     5461 atcttttgat ctgatcgtgc tcgacccgcc caaattcgca cccaccgccg ctcatgccga
     5521 aaaagcctcg cgggcttata aagacatcaa tttgctggcc ttcaagttgc tccgccgcgg
     5581 cggattgctt ttcacctatt cctgctccgg gggcatcgat gcggccctgt ttcaaaagat
     5641 cgtcgcctcc gccgccctcg atgcaggcgt ggatgcgacg atcattgagc atttgtcaca
     5701 gggcagcgat cacccggtca gtttgcattt ccccgaaggg atgtatttga agggactggt
     5761 ttgtgtgaag gggtgatttc cacttgcagg gtttaccacc tgtcagttaa aggcactgac
     5821 gatgaaaaac gccaggcaca ggaaacctat cataaaaata ctggggagaa agatgacccc
     5881 gatcgctttt ccggtggtca aagaatgagc ggcctgcaag gcgcggatat ttaacacaag
     5941 tccgtagatg ccaaccagac tgttgaagca ttggccgaca acggggatga tgccaatgac
     6001 ggcgatcaaa atggataaag gtgcaagata tgccgcttgt gcatagatcg tgcgggtgaa
     6061 atttccatgt ccgcccatga aagcggcgat caagttctgg aaccatgcgc tgatcgtcag
     6121 gctgatcaca ctcagcagcg gggatatcac agccattgcc agtgccagga tcaatgtaaa
     6181 ggcgaccgtt ccaaaccttt cgaagaactg attgagctca gacattgttt gcaattcggc
     6241 gaattgtggg ctggccccga gcgtaaatgg gaataacaat ccgctgatga tgcctgcata
     6301 ggctatccat tcaagggcgc ggcctgttgt tgcttcaggg tcattgagcg tgtcttcata
     6361 ggcatccatc cgcagcatgg agagtgcgat catccacact tcgtgaaacg cataggactt
     6421 tctcgttggc ttgggtttcg tgagtgaaaa agtatcggag taactgatgg tatctttttt
     6481 ctgcggtgtg gatgtgtaag cgtaatgatc tgtttcagcg gatggttcgt cgtcgaaata
     6541 gggatgtgtg gatgaagaag cctttggttt atcttcataa tacatgggct cataagacga
     6601 ggcggcaggt tttggaggct cgtctctgag gggtgcggaa taggtttggt gcgtcggttg
     6661 tgcaggcggc ggagtcactg cattttgggc tggccgctgg ctgcgtagtt tttgcaaagc
     6721 ctgcaagacc tgtgcattgc cggggttgat ctcaaggcag agttccagca cctggatccg
     6781 tttttcaagg gaggaatagg tctccacaga ccaaaaccac gcctggatat tccccggctc
     6841 cgaccgtaag atcaattcga gaatctttcg cgcttcttcc ttccgccctt cgcgaatcaa
     6901 ctggatggca ttgtttagac ctgacatagg acctcatgga aaatgcagaa tacggggtta
     6961 tgtggtcaac gaataaaaat gaacgatttg t
//
LOCUS       DAOQJC010000167         6939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_6056_length_6939_cov_11.560721, whole genome shotgun sequence.
ACCESSION   DAOQJC010000167 DAOQJC010000000
VERSION     DAOQJC010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6056_length_6939_cov_11.560721"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PLE39_17460"
     CDS             complement(<1..236)
                     /locus_tag="PLE39_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNE70175.1"
                     /translation="MTTTVSIIGGSGYGGGELIRLLLGHPNVEIKQVTSRSHLGEYVY
                     QVHPNLRKRTQLKFVDPAALEPVDILFLAQPHGQA"
     gene            complement(290..1153)
                     /gene="lysX"
                     /locus_tag="PLE39_17465"
     CDS             complement(290..1153)
                     /gene="lysX"
                     /locus_tag="PLE39_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HNE70176.1"
                     /translation="MQRRLKIGVLYSRVRVEEKWIFGAMEKRGIDYDRLDDRAIHFDL
                     EKPEAWQQYDAVLERSISYNSGLYALRLLNSFGVPTVNTAAVAEVCGDKLMTSAVLAR
                     DGVPQPHNVTAFSSEAALDAIEAFGYPVVLKPVVGSWGRLLAKINDRDAAEAVLEHKS
                     TLGSVQHSVFYIQEFIEKPGRDLRIIMIGDKVLTGMYRNSEHWITNTARGGSGELCPI
                     TPEIEDICLRASKAVGGGLLGVDLVEHPEKGLVVNEINHTMEFHTMQPVSGLDIAGEI
                     VEYVVKTAKER"
     gene            complement(1198..1368)
                     /gene="lysW"
                     /locus_tag="PLE39_17470"
     CDS             complement(1198..1368)
                     /gene="lysW"
                     /locus_tag="PLE39_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HNE70177.1"
                     /translation="MSTVVCPECEAQVELAAGTEAGEIIVCSDCGVDLEVTSLDPATV
                     ELAPMEQEDWGE"
     gene            complement(1372..2733)
                     /gene="argH"
                     /locus_tag="PLE39_17475"
     CDS             complement(1372..2733)
                     /gene="argH"
                     /locus_tag="PLE39_17475"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNE70178.1"
                     /translation="MTLWGGRFSQKLDDLAWALNASLPVDQHMAFQDVEGSIAWAAAI
                     HRAGILTDEEHASISTGLDTVRNEFASGEFIFQPSDEDIHTAVERRLSELIGPAAGKL
                     HTGRSRNDQVATDFRLWMLNKIPELHSAILILQNALVEQAELAGETIMPGYTHLQRAQ
                     PILLGHWWLSHYWALKRDVERLQDLTKRVSVLPLGSGALAGAPFALDRDALAESLGFD
                     EVSQNSLDAVSDRDFAAEYLFVCSMASVHLSKLAEQIVLYTTAEFGFFELSDAFSTGS
                     SLMPQKKNPDVFELTRGKAGTMIGLLTGLMSTLKGLPSTYDKDLQEDKAPVFQATDTL
                     LSILPVMAAALRTITVKSERMRGAIDSTMMATDLADYLVEKGVPFREAHSLAGKVVRA
                     AGEAKVRMEELPLEAYQAISPVFEADVYQVFDPLESINKRNTVGGTSLQSVKNQIKKI
                     KGE"
     gene            complement(2795..4066)
                     /locus_tag="PLE39_17480"
     CDS             complement(2795..4066)
                     /locus_tag="PLE39_17480"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNE70179.1"
                     /translation="MSPKSKPSVKKVVLAYSGGLDTSVIVPWLKENYGCEVVCFCADV
                     GQGDEDLAGLEQKAIKSGASEFVIHDMKEEFVSEYLFPMLKAGAVYEHKYLLGTSVAR
                     PLIAKHLVEVAHQTGADAIAHGATGKGNDQVRFELTVMALDPRLKIIAPWREWEIRSR
                     EDALNYAAKHNVPVTATIKSIYSRDSNLWHLSHEGGLLEDPWNEPEEVMFQMSTSPEN
                     APEQGEYVEIEFESGNPVSVNGEKMSPAKIVSTLNAIGGNHGIGRVDLVENRLVGMKS
                     HGVYETPGGTILLYAHRELESLILDRETLHYKQVIAVKYAELTYNGLWFTPLREALDA
                     FVNSTQGPVTGTVRLKLYKGNIISAGRKSKFSLYREDFATFGQEDVYDQSHAEGFIRL
                     YGLSMKVRALNGLPVSGLDMPKPDYSKFKRD"
     gene            complement(4385..5254)
                     /locus_tag="PLE39_17485"
     CDS             complement(4385..5254)
                     /locus_tag="PLE39_17485"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNE70180.1"
                     /translation="MKIHLVDGTYELFRAHFGAPPKKAANGMEVGATLGLLRSMLLLL
                     SDPDVTHVAVAFDHVIESFRNKMYKGYKSSAGVDPVILNQFPLAERAVSALGLVTWPM
                     VKYEADDAIATAAAKFKKSKQVEQVVICSVDKDLTQMVDGDRIVCWDRRREIILNEKG
                     VVEKFGVGPKSIPDFLALVGDSADGYPGIQGWGEKSTATVLANYRHIEAIPSDPAKFG
                     LGLGRATTLLENLKNNYEDALLFRELSTLRTDVPLKETLADLKWQGATQRLKKVCKEL
                     GSESILDRIKIWR"
     gene            complement(5280..5849)
                     /locus_tag="PLE39_17490"
     CDS             complement(5280..5849)
                     /locus_tag="PLE39_17490"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNE70181.1"
                     /translation="MRKIIVHEFITLDGVIQAPGSPTEDTDGGFTHGGWTIPYWHDDI
                     GAHFGQIFAEADTLLLGRKTWKLHGDAFEPNPAEDPFNGFKKYVVSSTLKSADGWRDS
                     TIISGDIIGEIRKLKNQPGGNIIMDGSSVLLHALFEHDLVDELSLHVYPLVLGSGKRL
                     FPSGKHISLKLIESSSLPTGIVYQRYQLA"
     gene            complement(5846..6460)
                     /locus_tag="PLE39_17495"
     CDS             complement(5846..6460)
                     /locus_tag="PLE39_17495"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNE70182.1"
                     /translation="MKQGDPNNNNGFPKIGAPAMRALEAAGYTNLKQLTKVTEVELAQ
                     LHGMGPKALGILRETLKAQGLSFKQGKAGVTLKKKGSPVSRTDKVDEFLENLSHPLTA
                     EVEAVRSIIKGVNKDINEEIKWRAPSFNYKGEYLVTFNLWEEKRIHLVFHNPLISQVK
                     SSLLEGEYKNRRMAYFADMKDVKAKKPLLEKALKDLIKLLEKKP"
     gene            complement(6473..>6939)
                     /locus_tag="PLE39_17500"
     CDS             complement(6473..>6939)
                     /locus_tag="PLE39_17500"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNE70183.1"
                     /translation="RKEQPYVAVRVQVGIPFGEVLGGFWGEAGAYLASKGMKPSGPPF
                     IRYLTTDMDKKLDIEVGFPVDNIVDGNDRITTGVFPAGQYAVYLYFGHYENLVAVTAE
                     FLAWAEKNNITWQTTKKDEVEWWVGRIEWYPTDPDNEPDPQKWQTELAFLVK"
BASE COUNT         1664 a         1918 c         1610 g         1747 t
ORIGIN      
        1 gcctgtccat gcggctgagc gaggaagaga atatccaccg gctccagcgc ggcagggtca
       61 acaaatttaa gctgagtccg cttgcgtaga ttcggatgga cttgatacac atattcgccc
      121 aaatgcgaac gcgaagtgac ttgcttgatt tcaacattgg gatgaccaag cagcaaacgg
      181 atcagttcgc cgccgccata tcccgaaccg ccgatgattg acactgtagt agtcaaaata
      241 tctcctttag gtaactggtg attagtaatt ggcaattacc agttaccaat taccgctctt
      301 ttgccgtctt caccacatac tccacgatct cgccagcaat atccaatccg ctgaccggct
      361 gcatggtgtg gaattccatc gtgtgattga tctcatttac tacgagtccc ttctcagggt
      421 gctccaccag atccacacca agcagaccac cgcccaccgc ttttgaagcc cgcaggcaaa
      481 tatcttcgat ctcaggtgtg atcgggcata gctcaccgct gccgccgcgt gccgtgttcg
      541 tgatccaatg ctccgagttg cggtacatgc cagtcagcac tttgtcaccg atcatgatga
      601 tgcgcaggtc gcgacccggc ttctcaataa attcctgaat ataaaagacc gaatgttgaa
      661 ctgaacccaa agtcgattta tgttccagca cagcttccgc cgcatcgcgg tcgttgatct
      721 ttgccagcaa ccgtccccac gaaccgacaa ccggcttcaa caccacgggg tagccaaatg
      781 cctcgatcgc atccagcgcc gcctcggatg aaaatgccgt cacattatga ggctgcggca
      841 cgccatcccg tgcaagcacg gcactggtca tcaatttgtc accgcatacc tcagccactg
      901 ccgccgtgtt gaccgtcggc acaccgaacg aattcaacaa acgcaaggcg tacaagccgc
      961 tgttgtaact gatactgcgc tccaacaccg catcatattg ctgccatgct tcaggctttt
     1021 ccagatcaaa gtgaatggca cggtcatcaa ggcggtcata atcaatgccg cgtttttcca
     1081 tcgcgccgaa gatccatttc tcttcaacgc gcacacggga ataaaggacg ccaattttca
     1141 atcgtcgttg cataatattc tccgtagggg cacgatacat cgtgccccta caataatcta
     1201 ttcaccccaa tcttcctgct ccatcggcgc aagctcaacc gttgcagggt ccaacgacgt
     1261 tacctcgagg tccactccgc aatccgagca gacgatgatc tcaccggctt cggttccggc
     1321 agccaattca acctgggctt cacattcggg gcatacaact gtagacatga tttattctcc
     1381 tttgatcttc ttgatttggt ttttgacaga ctgaaggctc gtgcctccaa ccgtatttct
     1441 tttattgatc gattccagcg gatcaaagac ctgataaaca tccgcttcaa acactggact
     1501 aattgcctga tacgcttcga ggggcagttc ctccatccga accttcgcct cccccgccgc
     1561 gcggacgact ttccctgcca gcgagtgcgc ttctctaaat ggcacgcctt tctccacgag
     1621 ataatccgcc aaatccgtcg ccatcatcgt ggaatcaatc gcgccacgca ttcgttccga
     1681 cttgaccgtg atcgtccgaa gcgccgccgc catgacaggc aggatcgaga gcaaggtgtc
     1741 cgtcgcctga aagacgggtg ctttgtcttc ctgtaaatcc ttgtcgtatg tagatggcaa
     1801 ccccttgagc gtggacatca accccgtcag caagccgatc attgtccctg ctttgccgcg
     1861 cgtcagctcg aagacatcgg ggtttttctt ttgcggcatc aagctcgaac ccgtggagaa
     1921 ggcatcggaa agctcgaaga aaccgaactc agccgtggtg tacaagacaa tttgctcggc
     1981 aagtttgctc aagtggacag acgccatcga acagacgaac aaatactcag cggcaaaatc
     2041 cctgtcagag acggcgtcga ggctgttctg cgaaacttca tcgaagccaa gagactctgc
     2101 aagggcatcg cggtccagcg caaagggagc gcctgccaaa gctccgcttc ccaaaggcag
     2161 aacggataca cgtttggtca ggtcttgtaa tcgttccaca tcgcgtttca acgcccagta
     2221 gtgactcagc caccaatgcc ccaacaggat cggttgcgct ctctgcaagt gggtataacc
     2281 gggcatgatg gtctcacccg caagttccgc ctgttcgacc aacgcatttt gaagaatgag
     2341 gattgcggaa tgaagttcgg gaattttgtt caacatccac aacctgaaat ctgtggcaac
     2401 ctgatcgttg cggcttcgcc cggtgtggag tttgccagca gcgggcccga tcagttcgct
     2461 tagtctgcgt tcaacggcgg tgtggatatc ctcgtctgaa ggttggaaga taaactcacc
     2521 cgaagcgaat tcatttcgga cggtatccag cccggttgag atcgaggcgt gttcttcatc
     2581 cgtaagaatg cctgcccggt gaatcgccgc agcccaggca atgcttcctt ccacatcttg
     2641 aaatgccatg tgttgatcga ctggcaggga ggcgttaagc gcccatgcga ggtcatctag
     2701 tttttgggaa aagcgtccgc cccaaagggt cataaaatct ccttggtaat tggtaaatgg
     2761 aaaatggaaa tttccattta ccaattactt ttcactaatc tctcttgaac ttcgaataat
     2821 ccggcttggg catatcgaga ccggaaacgg gcaggccgtt gagcgcgcgg actttcatgc
     2881 tgagaccata caagcgaatg aagccttcgg cgtgactctg gtcgtagaca tcctcctgac
     2941 caaaggtggc gaaatcttcg cgatagaggc tgaacttgga tttgcgacct gcgctgatga
     3001 tgttgccttt gtaaagtttg agccgcacgg tgccggtgac aggtccctgc gtggagttga
     3061 caaaagcatc gagcgcttcg cgcagcggtg tgaaccacag accgttgtaa gtgagttcgg
     3121 catacttgac cgcgatcact tgtttgtagt gcagggtttc gcggtcgagg atgagcgact
     3181 caagttcgcg gtgggcatag agcaggatcg tgccgccggg ggtttcgtac acgccatgtg
     3241 atttcatacc cacgagtcga ttctcaacca gatccacgcg gccaatgcca tgatttccgc
     3301 caatggcatt gagcgtggaa acgatcttcg caggcgacat cttctcgcca ttgaccgaga
     3361 cagggttgcc gctctcgaat tcaatttcca catactcacc ttgttcgggc gcattctcgg
     3421 gcgaggtgga catctggaac atcacttctt cgggctcgtt ccacgggtct tcgagcaggc
     3481 cgccttcgtg cgaaagatgc cagagatttg aatcgcgtga gtaaatggat ttaatagttg
     3541 cggtcacagg gacgttgtgt ttcgcggcgt agttgagcgc atcttcgcgg gagcggatct
     3601 cccactcgcg ccacggggca atgatcttca ggcgcggatc aagcgccatg acagtcaatt
     3661 caaagcgtac ctgatcgttg cctttgccgg tcgcgccatg tgcaatcgca tccgcgccgg
     3721 tctggtgcgc cacttctaca agatgtttcg caattaacgg acgggccacc gatgtgccca
     3781 ataaatactt gtgttcatat actgcccccg ctttgagcat cgggaaaaga tactcactga
     3841 cgaattcttc tttcatgtcg tgaatgacaa attcgctcgc gccactttta atggcctttt
     3901 gctccagccc cgccagatct tcatctccct gccccacatc cgcacaaaaa caaacgactt
     3961 cgcagccata gttttccttc aaccacggca cgatcacaga cgtatccaga ccgcccgagt
     4021 aggctaagac gactttcttg acactaggtt ttgactttgg actcattttt actccttgta
     4081 tggtaatgcc gacttaccca cagggcttag tcggcgttac gaaaaataaa aacagccctc
     4141 cgagaaggag ggctgtttgg gttgacttgt gactatgcag gcttagttca catttgcgtt
     4201 cgttccgccg tcaaaacaaa aaactcccga ccttctctgg gaagtgggca tgggacgacg
     4261 ggtacgaggc agtgcaaaca aattgaacat gttgggcggt attctactcg aaatgtgaaa
     4321 tgagtcaatg gttttgggcg ggtaaattca aagagtaccc gaatgggtac tctttggaaa
     4381 tcattcaacg ccagatcttt attcggtcga ggatactttc gcttcccaat tccttgcaaa
     4441 ctttcttcaa gcgctgtgtt gcgccctgcc atttcaagtc tgcgagcgtt tccttcaatg
     4501 gcacatccgt ccgcaaggtc gataactctc tgaaaagcag ggcatcttcg tagttatttt
     4561 ttaaattctc aagcaaggtg gttgcgcgcc ccaaacccag gccaaatttc gcaggatcac
     4621 tgggaattgc ctcaatatgc ctgtaatttg ccagcactgt tgcagttgat ttttcacccc
     4681 aaccctgaat gcccgggtaa ccatcggcag agtctccgac cagtgccaga aaatccggga
     4741 tcgacttcgg cccaacgcca aatttttcga ccacaccttt ttcattcaat atgatctcac
     4801 ggcgccgatc ccaacacaca atgcggtcac catcaaccat ctgggtcaga tctttatcga
     4861 ccgaacaaat gacaacttgc tccacttgtt ttgatttttt gaatttagcc gctgctgtag
     4921 cgatggcatc atccgcttca tacttgacca tgggccaggt caccaatccc aaagcagaga
     4981 cggccctttc agcaagtgga aactgattca agatgacggg gtccacacct gcgctggatt
     5041 tgtaaccttt atacatcttg ttccgaaacg actcgatcac atgatcaaat gcaacagcaa
     5101 catgcgtcac atcagggtct gaaagcagca gcaacatact gcgcagaagg ccgagcgtgg
     5161 cgccgacttc cataccattc gcggctttct tcggcggtgc gccaaaatga gcacgaaaaa
     5221 gttcataggt gccgtcaaca agatggattt tcatacaggc cctcatttac ttcatttttt
     5281 caggcaagtt gatatcgttg atacacaata ccggtaggca gcgaagaaga ttcgatcaac
     5341 ttaaggctga tatgttttcc gcttgggaac aagcgttttc cgcttccaag aaccagtgga
     5401 tatacatgca gtgaaagttc atccaccaga tcatgctcaa acaaagcatg gagcaggaca
     5461 ctgctcccat ccataatgat attcccgcca ggctgatttt tcaacttgcg gatctccccg
     5521 attatatctc cgctgatgat cgttgaatca cgccacccgt ctgctgattt taacgtggaa
     5581 gaaacgacat atttcttaaa accattaaat gggtcttcgg cggggttggg ctcaaatgca
     5641 tcgccatgaa gcttccatgt ttttcgtccc aacaatagcg tatcggcttc agcaaagatc
     5701 tggccaaagt gggcgccaat atcatcatgc cagtatggaa ttgtccagcc gccatgggta
     5761 aaaccgccat cggtatcttc agtgggactg cccggcgcct gaatgacgcc gtccaacgta
     5821 ataaattcat gaacaataat ttttctcatg gttttttctc cagtaatttg atcaaatcct
     5881 tcaatgcttt ttccagcaat ggcttcttcg ccttcacatc cttcatgtct gcaaaatacg
     5941 ccatgcgccg atttttgtac tcgccttcca acaacgaact cttcacctgt gagatgagcg
     6001 ggttatgaaa gacaagatga atacgctttt cttcccagag attaaaagtc accaggtact
     6061 cgcctttgta attgaaggat ggcgctctcc atttgatttc ctcattaata tccttgttca
     6121 cacctttgat gatggaccgc accgcctcca cctccgccgt aagcgggtga ctgagattct
     6181 caaggaattc atccacctta tccgtgcggc tgacgggcga gcctttcttc ttcagcgtta
     6241 cgcctgcctt tccttgttta aaagacaagc cctgcgcctt caacgtctcg cgcaaaatcc
     6301 ctaacgcttt cggacccatc ccatgcagtt gagcgagttc cacttccgta actttggtca
     6361 actgcttaag gtttgtgtat cccgccgcct ccaaggcgcg catggcgggc gctccgatct
     6421 ttggaaaacc attgttatta tttggatcac cttgtttcat acaaactcct gcttatttca
     6481 caagaaacgc aagttccgtc tgccactttt gcggatctgg ctcattatct gggtcagttg
     6541 ggtaccattc gattcgtccc acccaccatt caacctcatc ttttttcgta gtttgccagg
     6601 taatattatt tttttcggcc caagccagaa attcagcggt cacagccaca agattttcgt
     6661 aatgtccaaa atacaagtac acagcatatt gtccagcggg aaatacgcct gttgtgatgc
     6721 ggtcatttcc atcgacaatg ttgtcaacag gaaagccgac ttctatatcg agttttttat
     6781 ccatatcagt agtgagataa cgaataaaag gcggaccgga aggtttcatt cccttgctgg
     6841 ctaaataggc acccgcttca ccccagaagc ctccaagcac ctcaccaaag ggtatcccta
     6901 cttgtacgcg gactgctaca tatggttgtt ccttacgat
//
LOCUS       DAOQJC010000168         6909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_6088_length_6909_cov_9.808579, whole genome shotgun sequence.
ACCESSION   DAOQJC010000168 DAOQJC010000000
VERSION     DAOQJC010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6088_length_6909_cov_9.808579"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..4302
                     /locus_tag="PLE39_17505"
     CDS             346..4302
                     /locus_tag="PLE39_17505"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE70184.1"
                     /translation="MNILYTYIPQDRLRALAHGEDLPTHTSGSALFADISGFTPLTRA
                     FAEAFGPKRGAEALLGVLNPLFEALIEPVHRFGGSVIGFAGDAITCWFEASPRHSEPS
                     LYHSSLRATAVALAMQSVMKPFAAVHAPDGSLVTLSIKVAIAAGSARRFMVGDPAIQQ
                     MDTLAGITLSRMAEAEKYASQGEVVVSGEVAESLKDVLNVSAWRDEQRYAVVDGLKVE
                     VQAEPWPILTEDALSEVQVRQWVLPFIFEQLNSGVVNLGDLRPVTPLMVQFEGIDFDA
                     DENAGSKLDSFVSWSQRIVHQYGGSLIQLTIGDKGAFMYAVFGAPLAHENDPTRALQA
                     ALALRDMPVDLAAYLKPLRIGLTYGTAWTGNYGGYERFTYGVISSEVNLSARLMGKAE
                     PGQILVSGRMGQYPGFRFEHVGNLIFKGFDTPLPTYSLLGEQVSDAQAFQTAMVGRDD
                     ELEKLATFAQPLFANHLAGVAIVYGEPGIGKSRLAYALHQMFGERVSWFTGQTDQILS
                     QAFNPFIYWLKRYFDQSPNASMEQNKVNFESRLDQLGDSLQALTVSPHSPISNLQSEL
                     IRTRSFLGALLGFDWPDSLYAQLDSPKLRYENTLIAIKTLLLAESQLRPVVFELEDGH
                     WLDDPSREMLLAISRNVAAYPLLVLITSRYNDDGSQPVFALDREMPILKLDLEALTRE
                     ALERQAAAVLGGKISPALLALLWERSRANPFFAEQMLLHFHETGILIQTQMGEWEFQS
                     SPVTLSIDVHTMLVARIDRLTHKVRQVVQAAAVLGIEFDVQLLSKMLHTDALPEVQDA
                     ERGQIWQLMRELRYIFKHALLRDAAYDMQLRSRLRELHHLAAVTAEQVYEDQLSSYYD
                     ILAYHYRMAYQLGVENVSEQVCFYLRKAGEAAQKNFANSVALDYYNQLLPMLQDLSEQ
                     AQVYLQRGAVLELIGRWPEAENDYRTALDLVDGNTSTQAGAQLALGKLFHLRGDFSPA
                     LKWFEQAQMTWTTLQDQAGLSQTLIATGFVLWRKGDLQDARRYLEEGLTLARMLHDQP
                     GVALALEGLGRVAHVQDDYAASQALHTESLSLRRELDDKQGIAWSLNYLGIVAAGQGD
                     YVVAQALFEESLALFRDLGDKFGIAEVLEKLGITMRFRGEWGKARVFYEESLDISREI
                     GDRSSTAWVLYNLGAVAHSQKEYETARALLEEGLVHFRELGHKAGIAWVLSFLGKPLT
                     NLQQFNAARAAFREALVVFQQLQSTIGVIFSIEGVVNLSIAQGQLEESAQLIAWATAA
                     RESINNPRPVGEQEETDLALANLRTALGEDTFHTAWMEGGRMTMEEAIQLALHDVP"
     gene            4383..6050
                     /locus_tag="PLE39_17510"
     CDS             4383..6050
                     /locus_tag="PLE39_17510"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE70185.1"
                     /translation="MSEKKNQPDMQRILSISQELVADRPLDEILHRIVQVAADLVNCE
                     SVGILLSDESANTLRFVAATLYQDRLFDFPVPIDASIAGAAFISNELVNVPDVSVDPR
                     YYPKIADLLNFPARSLLAAPLKFKDRKIGVLEAQNKKDREEFDAVDVQIMTALAAQVS
                     IALEHARQMERYKQLAQDEQNQRQMAEAMRLASAALTSTLDYDQVIDNIMEQVSSVIP
                     LDTCNVMMIEKEDTARIARVYRGRGYEQVGTAATLQDTTLNISDVAGLRRMCETRQPV
                     LIFDVAQDLEWVYSRPEHHWIRSYIGAPIIIRDSVVGFLNVMSGTPNLYNQALAERLQ
                     AFVHYAATAIENARLYQQAQEEIAERIKAEEELRHHRDHLEELVKERTADIHRLAITD
                     SLTELFNRRHLISLGERAINLAQRYHHPLVAMMADLDHFKKINDTYGHAAGDEALRKL
                     AAQIRQVFRSTDILGRYGGEEFVVLMPETDIVSGHKIAERLLHLTQGVSISSDKIEFG
                     FTISIGLAERSQTNARTLDQLIDHADSALYNAKQSGRNRVEVYQVPR"
     gene            complement(6064..6654)
                     /locus_tag="PLE39_17515"
     CDS             complement(6064..6654)
                     /locus_tag="PLE39_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70186.1"
                     /translation="MKTNIEWNYSGKPDGFFGTGATQAEQNLVWLFGILSTALLGLYW
                     WSHAMDWSWWQYATALLLALDVLGGVVANSLNSCKRFYHSPIQPEETGFTALAKNHFA
                     FTLLHIHPILIGLVFGNMNLAYGIIWYVVMVIVSAGVQKLPLYLQRPTAMGVIMLAIL
                     INYYVIPPITGFEWFIPALFLKIVYGHIVQEEPYRA"
     gene            complement(6656..>6909)
                     /locus_tag="PLE39_17520"
     CDS             complement(6656..>6909)
                     /locus_tag="PLE39_17520"
                     /inference="COORDINATES: protein motif:HMM:NF033684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter suffix domain-containing protein"
                     /protein_id="HNE70187.1"
                     /translation="PPAAWMFPLGIGLVILSTLLYGGLIALPFFSIPTTTKLAVTPML
                     IFFGEATFWVGGALLGKEIVTRYKQYLNPCNWFCKPSAG"
BASE COUNT         1707 a         1558 c         1879 g         1765 t
ORIGIN      
        1 aatgggaatg gaggaaatca ttaatcagat gtaaaagtat ggcgctctac taaaaccaat
       61 aaaaacgcct ctgcttcggc aggggcgttt ttattgcaaa tagttaggca atatcaatct
      121 tgcggcaacc aacgttgtga gagattacat caccaggatg atatttggat tcgtttagat
      181 ggtggtagag gatattttca tgggaaagcc ttgttgagag ccgacgattt aagtcactgc
      241 gtttcagtgt taaacaaatc attgggtata aagaaatata ttaacttgac atgcttccca
      301 atttattgaa caattactga aacgaaatcc ctactcagga aactcatgaa tatcctgtac
      361 acctatattc cacaagaccg tttgcgcgca cttgcccatg gcgaggatct gcccacccat
      421 acatcgggca gcgccctgtt tgcggacatt tctggtttca ctccattgac ccgtgccttt
      481 gctgaagcgt ttggccccaa gcgtggtgca gaagccctat tgggtgtgct taacccgttg
      541 ttcgaggcgc tgatcgagcc agtgcatcgc ttcggcggca gtgtgatcgg ttttgcgggt
      601 gatgcaatta cctgctggtt cgaggcttct ccaaggcatt ctgaaccatc cctttatcat
      661 tcctccctgc gggcaacggc tgttgcgttg gcgatgcaat cagtaatgaa accatttgct
      721 gctgtacatg cacctgatgg ttcattagtt acgttaagca tcaaagtcgc gatagcggct
      781 ggttctgcgc ggcgttttat ggtgggtgac ccagcgattc agcagatgga taccctggcg
      841 ggcatcacat tatcacggat ggcggaggca gagaaatatg ccagtcaggg tgaggttgta
      901 gtcagtgggg aagttgcaga gagcttgaaa gatgtcctga acgtcagtgc atggcgcgat
      961 gagcagcggt atgcggtggt cgatggtttg aaggtggagg ttcaggcaga accctggccg
     1021 atattgacag aggacgcatt gagcgaagtg caggttcgtc aatgggtgtt gccattcatc
     1081 tttgaacaac tgaatagtgg tgtggtcaat ttaggcgatt tacgcccagt gacacctcta
     1141 atggtccagt ttgaaggcat tgacttcgat gcggatgaga atgccgggag taagttggat
     1201 tcgtttgtga gttggtcgca gcgtattgtt catcagtatg gcggcagttt gatccaattg
     1261 acgatcggcg acaagggtgc gtttatgtat gctgttttcg gagcacctct tgcacacgaa
     1321 aatgatccga cacgggcttt acaagcggct ttggccctgc gtgatatgcc ggtggacctg
     1381 gccgcttacc tcaaaccact gcgtattggt ttaacatatg gaacggcatg gactggaaat
     1441 tatggcggat atgagcggtt cacctatggg gttattagca gtgaagtaaa cctctcagca
     1501 cgcctgatgg gcaaggcaga gccaggacaa attttggttt cagggcgaat gggtcaatat
     1561 ccgggctttc gttttgagca tgtagggaat ctgattttca agggctttga caccccgctg
     1621 ccaacataca gtttgttggg tgagcaggta tcggatgcac aggcttttca gacagcaatg
     1681 gtagggcgcg atgacgaact ggagaaactt gcaacatttg cccagccgct cttcgcgaat
     1741 cacttggcgg gcgtggcaat tgtctatggt gagccgggca tcggtaaatc gcgtctggcg
     1801 tatgcattgc atcaaatgtt cggcgaacgg gtttcctggt ttaccgggca aaccgatcaa
     1861 atcttgagtc aggcgtttaa ccccttcatc tactggctta agcggtactt tgatcaatcg
     1921 ccaaacgctt caatggagca gaataaagtt aattttgaaa gccgcctcga tcaacttggc
     1981 gatagcctgc aagcattgac cgtttcgccc cactcgccaa tctccaattt gcaatctgaa
     2041 cttatccgta cgcggtcctt tctaggtgca ttgttgggct ttgattggcc tgattcactt
     2101 tatgcacaat tggacagccc aaaattacgc tacgaaaata cgcttatcgc catcaaaaca
     2161 ctgttgttgg cagaaagcca gctgcgacct gttgtatttg aattggaaga cgggcactgg
     2221 ttggatgacc cttcgcgtga gatgttgctt gcaattagcc gtaatgtggc tgcataccct
     2281 ctgcttgttc tgatcacctc acgctataac gatgatggca gtcagcctgt ctttgcgttg
     2341 gatcgggaaa tgcccatact caaacttgat cttgaggcac ttactcgtga ggcattggaa
     2401 cggcaggctg ctgccgtctt gggaggaaaa atatccccag ctttgttggc tttactgtgg
     2461 gaaaggagtc gcgccaatcc cttctttgca gaacagatgt tgctgcattt tcacgaaacg
     2521 ggcatattga tacaaaccca gatgggtgaa tgggaattcc agtcttcgcc tgtcactttg
     2581 tccatcgatg ttcacacaat gctggtggca cgcattgacc gtttaacaca caaggtcagg
     2641 caggtggtgc aagccgctgc tgtgttgggg atcgagttcg atgtgcaatt gctctcgaag
     2701 atgttgcata ccgatgcctt gcccgaggtc caggatgcgg agcgcgggca aatctggcag
     2761 ctaatgcgcg agttgcgata tatcttcaaa catgcgcttt tgcgggacgc agcctatgat
     2821 atgcagttac gttcccgtct gcgagagtta catcatctgg cagcagttac tgcagaacaa
     2881 gtatatgaag accaactgtc atcctattat gacatcctgg catatcatta ccgcatggca
     2941 tatcaattgg gggtggagaa tgtgtctgag caggtatgtt tttatttgcg caaggcgggc
     3001 gaggcagcac aaaagaattt tgccaactct gttgcactgg attactataa tcaactatta
     3061 cccatgcttc aggatttatc cgaacaagca caagtgtatt tgcaacgagg ggcggtactg
     3121 gaactgatcg gcagatggcc cgaggctgaa aatgactatc ggacagcatt ggatttggtg
     3181 gatgggaaca cgagtacgca agcaggggcc caattggcgc tggggaagtt gtttcacctg
     3241 cgcggcgatt tttcccctgc gctgaaatgg tttgaacagg cacagatgac atggacaacg
     3301 ctgcaggatc aggctggttt gagccagaca ctgattgcaa ccggctttgt cttatggcgc
     3361 aaaggtgacc ttcaagatgc ccgtcggtat ttggaagaag gcctgacatt ggcacggatg
     3421 ctccatgacc aacccggtgt agccctggca cttgaaggtt tagggagggt ggcacacgtc
     3481 caagatgact atgcggcgtc acaggcgttg cacacagaga gcctgtcgct ccgccgcgaa
     3541 ctggacgata agcaggggat tgcgtggtcg ctcaactatc tgggaattgt ggctgctggt
     3601 cagggtgact atgttgttgc gcaggcatta ttcgaagaga gtctggccct ttttcgtgat
     3661 ttgggtgata agtttggcat tgcagaggtg ctcgaaaaat tggggataac aatgcggttt
     3721 cgaggggaat ggggcaaggc acgggtattt tatgaagaga gcctcgatat tagcagagaa
     3781 ataggtgatc ggtcatccac agcctgggtg ctatacaacc tgggtgcggt ggcacacagc
     3841 caaaaggagt atgaaacggc acgggccttg ttggaagaag gtctggtaca ttttcgtgag
     3901 ttgggtcata aagcgggtat tgcctgggta ctctcttttt tgggtaaacc actgacaaat
     3961 ttgcagcaat tcaatgcggc ccgtgcagct ttccgtgagg cgttggttgt ttttcagcaa
     4021 ttgcaaagca ccatcggtgt gattttttct atcgagggtg tggtgaattt gtccattgcg
     4081 cagggacaat tagaagaatc cgcccaattg attgcctggg ctaccgccgc ccgagaatcc
     4141 attaataacc cacgccccgt cggtgaacag gaagaaaccg accttgcact tgcaaacctt
     4201 cgcactgcac tgggtgaaga cacctttcat acagcctgga tggaaggcgg gaggatgacg
     4261 atggaagaag ccatccaatt ggccttgcat gatgtcccat gatctattta tgtgtttgat
     4321 ttttgaaagg tcatctatat cccctgatat attggaaggt tttagggcgt ataatttccc
     4381 tcatgtctga aaaaaagaac cagcctgata tgcagcggat cctgtcgatc agtcaggaac
     4441 ttgttgcaga ccgtccactt gatgagatcc tgcatcgaat tgttcaagtt gcagctgacc
     4501 tggtcaattg tgaatcggtt ggtattcttt tgtcagatga atctgcgaat accctgcgct
     4561 ttgtcgctgc gactttgtat caggatcggc tttttgattt tccagtgccc attgacgcgt
     4621 ctattgccgg ggcggcgttt attagcaatg aattggtaaa cgtaccggat gtcagtgttg
     4681 atccgcgcta ttatcctaaa attgccgacc tgctcaattt cccggcgcgt tctcttctgg
     4741 ctgctccact caaattcaaa gatcgcaaaa ttggtgtttt ggaagcgcaa aacaaaaagg
     4801 acagggagga atttgatgca gttgatgtcc agatcatgac ggcacttgca gcgcaagtat
     4861 ccatcgcgct tgaacatgcg cggcagatgg aacgctacaa acaactggct caggatgaac
     4921 agaaccagcg gcaaatggcc gaagccatgc ggctggcaag tgcggcattg accagtacac
     4981 tggattatga ccaggtgatc gacaatatca tggagcaggt cagttctgtt attccattgg
     5041 atacctgcaa tgtgatgatg atcgaaaaag aagataccgc ccgtattgcc cgggtatatc
     5101 gcgggcgcgg ttatgaacag gttggcactg ccgccactct gcaagacacc accctgaaca
     5161 tctcggatgt agctggtttg cgccgtatgt gtgagacccg tcagcctgtc ttgatctttg
     5221 atgtggcaca ggatttggaa tgggtctatt cgcgcccgga acaccattgg atccgatcgt
     5281 atattggcgc acccatcata atacgagact cggtggtcgg ttttctcaat gtcatgagcg
     5341 gaacaccaaa tttgtacaac caggctcttg ctgaacgatt acaggctttt gttcattatg
     5401 cggctaccgc cattgaaaat gcgcggttgt accaacaggc acaggaggaa atcgccgaac
     5461 ggatcaaggc agaggaggaa ttgcgccatc atcgtgatca tcttgaagag ttggtcaagg
     5521 agcggacggc cgatattcac cgtcttgcaa ttaccgattc gcttaccgaa cttttcaacc
     5581 gccgccatct catcagtttg ggggaacggg ccatcaatct tgcacagcgc tatcaccatc
     5641 ctttggtggc catgatggca gacctcgatc actttaaaaa gatcaacgac acctatggac
     5701 atgctgctgg ggatgaggcc ttgcgcaaac tggctgctca aatacgtcag gtctttcgca
     5761 gtactgacat tttagggcgt tatggcgggg aggagtttgt tgttctgatg cctgaaacgg
     5821 atattgtcag tggacataaa atcgccgaac gtttgctgca tttgacccaa ggggtgagta
     5881 taagcagtga caaaattgaa tttggattta ccattagcat tggcctggca gaacgatctc
     5941 agaccaatgc tcgaacgctc gatcaactta ttgatcatgc cgatagtgcc ttgtacaacg
     6001 ccaagcaatc aggacgcaac cgggttgagg tgtatcaagt tccaaggtga aataccgttt
     6061 gcattaagcg cgatagggtt cttcttgcac aatatgcccg tatacgatct taagaaacaa
     6121 tgcaggaatg aaccactcga accctgttat aggtgggatc acgtaataat tgatcaaaat
     6181 tgccagcata atgactccca tagccgtagg gcgctgaaga tatagcggca acttttgaac
     6241 tcctgcggaa acaatgacca tgactacata ccagatgatg ccataagcca gattcatgtt
     6301 tccgaacaca agcccaatga ggatagggtg aatgtggagg agagtgaatg caaaatggtt
     6361 ctttgccagt gcggtgaaac ccgtctcttc gggttggatg ggggagtgat aaaagcgttt
     6421 gcaactgttg agcgagttgg cgaccacgcc gcctagcacg tctaacgcaa gcaggagggc
     6481 agtggcatat tgccaccaag accagtccat tgcgtggctc caccagtaca agccaagcag
     6541 agcagtagaa agaatgccaa acagccagac gagattttgc tctgcctgtg ttgcgcctgt
     6601 gccgaaaaat ccgtctggct tgcctgagta attccattcg atgtttgttt tcatgttaac
     6661 ctgcactggg tttgcaaaac caattgcatg ggttgagata ttgtttatag cgcgtgacga
     6721 tttctttgcc gagcaacgcg ccgccgaccc agaaggtagc ttcgccgaaa aagatcagca
     6781 tgggcgtgac ggcaagtttg gttgtggtcg gaattgaaaa gaaaggcagc gcgatcaagc
     6841 cgccatacaa cagcgtggaa agaatcacca gcccgatgcc cagcgggaac atccatgcgg
     6901 cgggcggcg
//
LOCUS       DAOQJC010000169         6868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_6139_length_6868_cov_11.591369, whole genome shotgun sequence.
ACCESSION   DAOQJC010000169 DAOQJC010000000
VERSION     DAOQJC010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6868
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6139_length_6868_cov_11.591369"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..303
                     /locus_tag="PLE39_17525"
     CDS             4..303
                     /locus_tag="PLE39_17525"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: protein motif:HMM:NF014368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HNE70188.1"
                     /translation="MKIVAITSCPTGIAHTYMAAEGLEKGAKQLGHIIKVETQGSIGV
                     ENRLTENEIAEAELVIIAADVKVDLSRFTGKKIYETASNAAIKDGVGVINAALNS"
     gene            322..1410
                     /locus_tag="PLE39_17530"
     CDS             322..1410
                     /locus_tag="PLE39_17530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HNE70189.1"
                     /translation="MSTSSNSNSFSGAYKHLMTGVSYMIPFVVAGGLLIAIGFAARGI
                     YAYDATLALDGSTMEATAWTTFWANIFAIGAKGGFGLMVAILAGYISYSIADRPGLAP
                     GMIGGTLAGVTGSGFLGGIIAGFLAGYFTQWLNGAIKLPRNLSGLKPVLILPLISSLV
                     VGLVMFYVVGVPVSAALKALTAWLTGMQASSAAILGLILGAMMAFDMGGPVNKAAYTF
                     SVGLLSTQVTAPMAAVMAAGMTPPLGLALAAYLFKNRFTEDERESASSTAILGISFIT
                     EGAIPYAARDPFAVIPSIMVGSAVAGALSMAFGCQLHVPHGGIFVLPIPNAVDNLLMY
                     VVALLVGTAVTAGMLYLLKKPVEQAPAA"
     gene            1486..2481
                     /locus_tag="PLE39_17535"
     CDS             1486..2481
                     /locus_tag="PLE39_17535"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE70190.1"
                     /translation="MKTLVTGATGFVGGALARRLHGMGWNVTALGRNPAKLDQLEEEG
                     IHPLRADISKKDELTDSFLNLDLVFHCAALPSPWGNFEKFYQSNVIGTRNVVQACLEN
                     KVKRLVYVSTPSLYFDYGSRIGVKENDPLPEPISSYAHTKLLAEEEIDKGFAQGLAVV
                     SIRPRALFGEGDTVIFPRLIPRLRSGRLPILGDGENVVDLTYIENVVDALLLCAESPA
                     STLGKKYNITNDEPVKIWKLIERICDELGYRHPSRKISYKTANAAASLLELFYSLIPF
                     SPEPPLTRLSVSMMANSTTLDISAAKNELGYQPKISVEEGVQRFLKWWKEANPSL"
     gene            2520..3362
                     /locus_tag="PLE39_17540"
     CDS             2520..3362
                     /locus_tag="PLE39_17540"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE70191.1"
                     /translation="MKINILHAGYCTAPEHIAMQGGRWRTIHFPAMFALFRHPKFGAM
                     LFDTGYSYRFFDETKKFPKRIYRWMTPVHLKEEDLVVNQLSAFNLQPSDISHVFVSHF
                     HADHVGALCDLPKSRYVYFPHAFAHLRHLPPEEDMKYAYLRGQIPTDFDARSQEVDMA
                     RPVLLAEEYAPFKTGYDLLGDGSIIGVELPGHALGQMGIFARDENGKLFFFVADAAWL
                     KQSIVTNRPPHKIANLLFPDPALYRETLGQLHTYYLTHPDTVVVPSHCDETIRLLENA
                     PQQG"
     gene            3381..4544
                     /locus_tag="PLE39_17545"
     CDS             3381..4544
                     /locus_tag="PLE39_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70192.1"
                     /translation="MSNLSGVMTHPPQLFTRENIHTLPFPSTDDGEYARHYLIPLMTG
                     DPQKYIRNVYSTQLMAVKVGETTIPITVSDFHPQNTYTVSPYSHYVSYGAFEEVKHLN
                     NAAAEFAVKAVMNPLAWYFRKAELDRVVFVNNYLLSTNLYPSVNSDQLSAASEALIGW
                     FPDRAIVFRSVDQKKNPHVYRTLEGLGYDLVLSRQVWYMDPVESLKTRQCREDVRVLK
                     KNGYETVNGKDLSDAELHRAVELYNLLYLEKYSYYNPQFTFEFLKLARDHAILHMVGL
                     KKDGQLNGIMGFFVRNGAMTQPLFGYDTSLPMDEGLYRLLTLVTLQEGVKRGLLVHAS
                     GGVGKFKKVRGGESVTEYNAVYTRHLPKRRQLPWKLIGKVSNWAIPYFKKMDF"
     gene            4631..5626
                     /locus_tag="PLE39_17550"
     CDS             4631..5626
                     /locus_tag="PLE39_17550"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNE70193.1"
                     /translation="MQVNIPLKIIGLGRYLPKRVVPSSELEAMCGVPAGWVERRNGVR
                     ERRWVTDETSSFMSAEAAREALSEAKLKPEQLDLIINASGTGEQAIPDTGVLIQRQLG
                     LGASGIPAMTVHTTCLSFVAAMDVASNFISSGRYKNILICSADVASCGINPKEPESAS
                     LVGDAAAAVVVTRAGAGEASQIHHAHFKSFGDGAYLTTIMGGGSRLHPRFAGHNPEDD
                     LFHMDGPAVLRMVRGIAHEFLEELYPGLSNSMVDVDVVVPHQASKVGLMMLERFGWKD
                     EKIIKTIETLGNCVAASIPVTLYQGVRDGRIQRGNKVLLVGTGAGLSIGGMVLTY"
     gene            5703..6842
                     /locus_tag="PLE39_17555"
     CDS             5703..6842
                     /locus_tag="PLE39_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70194.1"
                     /translation="MANILLTGGRAPATLDLARVFHRAGHAVFMAESLRGHLSQPSKA
                     IKANFVVPAPRQNRDAFLFALKKIVTENQIDLLVPTCEEIFHTAMGHESLPLFSEPIE
                     KLRRFHNKWEFVVQAVENELHVPETMLIQNKEDLLHAYAQWRGLVLKPAYSRFAARTL
                     ILPPLQMALSTLKFTKGQPWVAQQYVEGQAFCTYSVCHKGRITAHTTYPSHFTAGRGA
                     TIVFKHVEHSAIFNWVRSFVSENQFTGQIAFDFIETPDGQVTALECNPRATSGVHLLA
                     SHPRFAESFFNPFMDCITPVDDSSFMLSAAMLLYGLPAALKNGSLKDWRKAYFFSNDV
                     ILDFNDPLPFILQWRSVLATRAIAKREKLSLLEASTFDIEWNGEG"
BASE COUNT         1508 a         1733 c         1878 g         1749 t
ORIGIN      
        1 tgaatgaaga tcgttgcaat tacctcatgc cccacaggga tcgcccatac ctacatggct
       61 gccgagggtc ttgaaaaagg cgcaaaacaa ttgggtcaca tcatcaaagt tgagacacaa
      121 ggctccatcg gtgtggagaa tcgcctgacc gaaaacgaga tcgcagaagc ggaattggtc
      181 atcattgccg ccgatgtcaa agtggacttg agccgtttta ctggcaagaa gatctatgaa
      241 actgccagca atgcggctat caaagatgga gttggcgtca ttaacgccgc tctgaattca
      301 taattttcaa ggagagagaa aatgtcaaca tcaagcaata gcaattcgtt ctcaggtgct
      361 tacaagcacc tcatgacggg cgtgtcctac atgatcccgt tcgtcgtcgc aggcggtctt
      421 ttgatcgcca tcggtttcgc tgctcgtggt atctatgcct acgatgccac cctcgccctc
      481 gatggctcca ccatggaagc caccgcctgg acgaccttct gggcgaacat ctttgccatt
      541 ggcgccaagg gcgggtttgg tctcatggtt gcgattcttg cgggctacat ttcctattcc
      601 attgctgacc gccccggtct ggccccaggc atgattggcg gtacccttgc aggcgttacc
      661 ggttcaggct tcctcggcgg cattatcgct ggcttccttg ctggctactt cacccagtgg
      721 ttgaatggcg cgatcaagct tccgcgcaac ctctctggtt tgaagcctgt gctcattctg
      781 cctctcatca gctcgctggt cgttggtttg gttatgttct atgtcgtcgg tgtacctgtc
      841 agtgctgcac tcaaggcact cactgcatgg ttgaccggta tgcaagccag cagcgccgcc
      901 attctcggtt tgatcctcgg cgcaatgatg gccttcgaca tgggcggccc cgtcaacaag
      961 gcggcttaca ccttctctgt tggtttgctc agcacacagg tcaccgcccc gatggctgct
     1021 gtcatggccg ctggcatgac cccgcctctc ggtttggctc ttgccgcgta cctcttcaag
     1081 aaccgcttca ccgaagacga acgtgaatcc gcttcctcca ccgccatcct cggcatttcc
     1141 ttcatcactg aaggcgcgat tccctacgcc gcacgcgacc cgttcgcggt gatcccctcc
     1201 atcatggtcg gctccgcggt tgctggtgcg ctctccatgg cgtttggctg ccagcttcat
     1261 gttccgcatg gcggcatctt cgtattgccc atcccgaacg cagtagataa cctgctcatg
     1321 tacgtggttg ccctgctcgt cggcaccgct gtcaccgcag gaatgctcta cctcttgaag
     1381 aaaccagttg aacaggctcc cgctgcctaa tccagaagtt tgatccatcc cccacccttg
     1441 tggtggggga tttttgtttg acccaattgg cgataaaatc agcttatgaa aaccctcgtc
     1501 acaggtgcaa caggatttgt cggcggcgct cttgctcgtc gcttgcatgg catgggctgg
     1561 aatgtgaccg cacttggacg caacccagcg aaactggatc agctcgaaga agaaggaatc
     1621 cacccgcttc gtgccgatat ttccaaaaag gatgaactga ccgattcctt tcttaacctt
     1681 gatctggtct tccattgtgc ggcattgccc tccccgtggg gaaattttga aaaattctat
     1741 caatccaatg tcatcggtac gcgcaacgtt gtgcaggctt gtctggaaaa caaggtcaag
     1801 cgtctggtct atgtctccac acccagcctt tactttgatt atggttcacg cattggcgta
     1861 aaagaaaatg acccgctccc ggaacccatc agtagttatg cccacacaaa gcttttagcc
     1921 gaagaagaaa tcgacaaagg tttcgcgcag ggacttgccg tggtgtccat tcgcccgcgt
     1981 gcgttgtttg gcgagggcga tacggttatt tttccgcgcc tgatcccacg cttgcgctcg
     2041 ggcaggcttc ccattttggg tgacggtgag aatgtcgttg acctgaccta tatcgaaaat
     2101 gtggtggatg cgctgttgtt atgcgcggaa tcgccagcca gcacgcttgg caaaaaatac
     2161 aatattacga atgatgaacc tgtcaaaatc tggaagctga tcgaacgcat ttgcgacgaa
     2221 ttggggtatc ggcatccgtc gcgcaagatc tcctataaaa ctgcaaatgc cgccgccagc
     2281 ctgcttgaac tgttttattc gcttatcccg ttcagccccg agccgccgct gacaagactc
     2341 tctgtcagta tgatggcgaa cagcaccacg ctcgatatct ctgctgcaaa aaatgaactt
     2401 ggttatcaac ccaaaatctc cgttgaagaa ggtgtgcagc gctttttgaa atggtggaag
     2461 gaggccaatc cgtcattgtg agggtgatag cccgaagaag caatgacgga aacattcata
     2521 tgaaaatcaa tattcttcat gcaggctatt gcactgctcc cgaacacatc gccatgcaag
     2581 gcggacgctg gcgcacgatc catttccctg ccatgttcgc attattccgc cacccgaaat
     2641 tcggcgcaat gcttttcgat acaggctatt cctatcgctt tttcgatgaa accaaaaaat
     2701 tcccaaagcg catttatcgc tggatgacac ccgttcacct gaaagaagag gacctggttg
     2761 taaatcaact gtccgctttc aacctgcaac cttccgatat ctcacatgtg tttgtctccc
     2821 acttccatgc ggaccatgtt ggggcgcttt gcgacttgcc caaatcgagg tatgtgtatt
     2881 ttccgcacgc gtttgctcat ctgcgccacc tgccgcctga agaagacatg aaatatgcct
     2941 acctgcgcgg acagatcccc acagatttcg atgcccgctc acaggaagtg gatatggcca
     3001 ggcctgtgtt actggccgag gaatatgcgc ccttcaaaac gggttatgac ctgctcggcg
     3061 acggctccat cattggtgtg gaattgcccg ggcatgctct ggggcaaatg ggcatcttcg
     3121 cccgcgatga aaacgggaag ctgtttttct tcgtagcaga tgccgcctgg ctgaaacaat
     3181 ccattgtgac caaccgcccg ccgcataaaa ttgccaacct ccttttcccc gaccctgctt
     3241 tgtaccgcga aacgctggga cagttgcaca cctattacct gacccacccc gatacagtgg
     3301 ttgtcccgtc gcattgtgac gagacgatcc gtttgctgga aaacgcgccg caacagggtt
     3361 gatgagccct gagagaagca ttgtctaact tatctggtgt gatgacgcac cctccacagc
     3421 tttttacccg tgaaaatatc cataccctgc cgtttccatc caccgacgat ggtgagtatg
     3481 cccgccatta ccttatccct ttgatgaccg gggacccgca gaaatacatc cgcaatgtgt
     3541 attccacaca gttgatggcg gtcaaagttg gagagacaac aattcccatc acggtttcag
     3601 attttcatcc gcaaaacacc tacacggttt cgccgtatag ccattatgtt tcgtatggcg
     3661 cgttcgagga agtgaagcat ttgaataatg cggcggcaga gtttgctgtg aaggcggtga
     3721 tgaatccgct ggcgtggtat ttccgaaaag ccgaattgga cagggtggtt tttgtcaata
     3781 actacctgct ttcgacgaac ctgtatccat cagtaaacag cgaccagttg tcggcggcga
     3841 gcgaagcgtt gatcggctgg ttccctgatc gggcgattgt gttccgttcg gtggatcaga
     3901 agaaaaatcc gcatgtttat cgaaccttgg aagggcttgg ttacgatctg gttctcagcc
     3961 gtcaagtctg gtatatggac cctgtggagt ctttgaagac gcgccaatgc agggaggatg
     4021 tgcgcgtgtt gaagaagaac ggatatgaga cggtgaatgg taaagacctt tcagatgcgg
     4081 aattgcatcg cgcggtggag ttgtataacc tgttgtatct tgaaaaatat tcctactaca
     4141 acccgcagtt cacatttgag tttttgaaac tcgcgcgcga ccatgcgatc ttgcatatgg
     4201 ttggacttaa gaaggatggg cagttgaatg gcattatggg attcttcgtg cgcaacggcg
     4261 caatgaccca gccgcttttt ggctacgata cctctctgcc gatggatgaa ggtttgtacc
     4321 gcctgttgac attggtgact ttgcaggaag gtgtgaaacg cggattactc gtccatgcca
     4381 gcggcggggt tggtaaattt aagaaggtgc gcggcggtga gtcggtgacg gaatacaatg
     4441 cggtgtacac acggcatttg ccaaagagac gccagcttcc atggaagttg atcgggaagg
     4501 tttcgaattg ggcgataccg tattttaaga agatggattt ttaaccttga tgaacggaga
     4561 taaacagaga taaagcctgg tttacatctt tgttcatccc tgtttatcga ggtaactttt
     4621 aggagcaaac atgcaagtaa acattcccct caaaatcatt ggtttgggaa ggtatttacc
     4681 gaagcgggtc gtgccgagtt cggaattgga agcgatgtgc ggtgtgcctg cgggttgggt
     4741 ggagcggcgc aatggggtgc gcgaacgtcg ttgggtgacg gatgaaactt cgtcgtttat
     4801 gtcggcagag gcggcgcgtg aagcattgag cgaggcgaaa ttgaagcccg aacagcttga
     4861 tctcattatc aatgcttcgg ggacgggtga gcaggcgatt cccgatacgg gggtgttgat
     4921 tcagcggcaa ttggggctgg gtgcttcggg catccctgcc atgacggtcc acaccacttg
     4981 tttgagtttt gtggcagcga tggatgtggc ttcgaatttc atcagcagcg ggcgttataa
     5041 aaacatcctc atttgcagcg ccgatgtggc ttcgtgcggc attaacccca aagagcctga
     5101 gtcggcttcg cttgtgggtg atgcggctgc agcagtggtg gtgacacgtg ccggggcggg
     5161 agaggcttcg cagattcacc atgcgcattt caaatcgttt ggcgatggcg cgtatctcac
     5221 cacgatcatg ggcggcggtt cgcgtttgca tccgcgcttt gcggggcata accccgagga
     5281 cgatctgttt cacatggatg gccctgctgt gctgcgcatg gtgcgcggca ttgcgcatga
     5341 atttctggaa gagttgtacc cggggctttc aaacagcatg gtggatgtgg atgtggttgt
     5401 tccacatcag gcaagcaagg tggggttgat gatgctggag cggttcggct ggaaggatga
     5461 gaaaattatc aagacaattg aaacgcttgg gaactgtgtg gcggcttcaa tccctgttac
     5521 actgtatcaa ggggtgcgcg atggacgcat tcagcgcggc aacaaggtgc tgttggtcgg
     5581 cacgggcgcg ggtttgtcca ttggcgggat ggtactaact tattaaccgc taaacactcg
     5641 aagagcgcga agaaaacctt tttgaatctt tgcgtccttt gcggtcttcg cggtaaacaa
     5701 caatggcaaa cattttatta accggcgggc gtgcgcccgc aacacttgat ctggctcgcg
     5761 tgtttcatcg tgcgggtcat gctgtcttta tggcagagtc tttgcgcgga catttgagtc
     5821 agccgtcaaa ggcgatcaag gccaattttg tggtgccagc tccacgtcag aacagggatg
     5881 cgtttctttt cgcgttgaaa aagatcgtga cggagaatca gatcgatctg ctggttccga
     5941 cctgtgagga gatattccat acggcgatgg ggcacgagag cctgcctttg ttttcagagc
     6001 cgatcgagaa gttgaggcgc ttccataaca agtgggagtt cgttgtgcag gcggtggaaa
     6061 acgagctgca tgtgcccgag acgatgttga tccaaaacaa ggaggatctg ctccacgcgt
     6121 atgcccaatg gcgcggactg gtgctgaagc ctgcttattc gcgtttcgct gcgcggacct
     6181 tgatcctgcc tccgcttcaa atggcattat ccacactgaa gtttacaaaa gggcagccgt
     6241 gggtggcaca gcaatatgtg gagggtcagg cgttttgtac gtatagtgtc tgccataaag
     6301 ggcggatcac tgcgcatacc acctatcctt cgcattttac ggcagggcgt ggggcgacga
     6361 tcgttttcaa gcatgtggag cattcggcga tctttaattg ggtcaggtct tttgtgagcg
     6421 agaatcaatt tacgggtcag atcgcttttg attttatcga aacacccgat ggacaggtta
     6481 ctgccctgga atgtaatccg cgtgccacga gtggagtgca tttgctggct tcacacccgc
     6541 ggtttgctga gtcttttttc aatccgttca tggattgcat tacgcctgtg gatgattctt
     6601 cgttcatgct ttcggcggca atgctgctct atggcctgcc cgccgccttg aaaaacggca
     6661 gcctgaaaga ttggagaaag gcttacttct tcagcaatga tgtgattttg gatttcaatg
     6721 atccgctgcc gttcattttg caatggcgca gtgtgctggc cacgcgcgca attgcaaaac
     6781 gtgagaagct ctcgctgttg gaggcttcca ctttcgatat cgagtggaat ggggagggat
     6841 agttgttgtt tgggggcgta taatttga
//
LOCUS       DAOQJC010000170         6861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_6149_length_6861_cov_9.983985, whole genome shotgun sequence.
ACCESSION   DAOQJC010000170 DAOQJC010000000
VERSION     DAOQJC010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6149_length_6861_cov_9.983985"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /gene="nrfD"
                     /locus_tag="PLE39_17560"
     CDS             complement(<1..1229)
                     /gene="nrfD"
                     /locus_tag="PLE39_17560"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNE70195.1"
                     /translation="MKTILKTIRENIFTPWGLWLGFLGMFLVAALVAGLSVFWFGLSL
                     TNLTDIVPWGLWITIDLSSIAVAAGAFSLCAIVYLFGFKRYEPIARTATFIGFTGYSM
                     AMMALLLDIGRPDRFWKSFVYWNTHSLLWEVTMCVGLYFTVLVLEVLPIVASFDWLRK
                     KFPRIAEKMEHLHHYAPVLAIFGLGLSSLHQSSLGAVYGVIKARPIWYRPEMSVMFMF
                     TAILGGVALTLFASMLSARLTSKAKVNDILIERVSQVLGYMMIAYLYWRFWDWLAQTY
                     TYEPGRTEAFELLTKGTLSFNFWFGEMLFGALIPTVILLYKPTRLSPFWRMLGLAMIV
                     GGVVAFRFDTNIVGFLAVIPYLPGEAVISYTSYSPSLVEWAAGLGIIAFGLLAFSIGV
                     KYLRVVDHRLTSEEYETV"
     gene            complement(1231..2010)
                     /locus_tag="PLE39_17565"
     CDS             complement(1231..2010)
                     /locus_tag="PLE39_17565"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNE70196.1"
                     /translation="MTENNLNRRDFIKISAVGLAVAAGMRVAQGEEVGASSATKGEHQ
                     WVMVIDQSKCTGCEQCNLACHAHNDINPELQWTKVLDAGEVNGEKVYLPRPCMQCEHA
                     PCVEVCPVGASYYRDDGIVMMDYDKCIGCRYCMVACPYDARAFNWKEFTDPNPAVPEW
                     GEPEVDRRPRGVPEKCSFCYNRIDRGMSVGLVPGVDAEATPACVVTCPTGARIFGDLN
                     DPHSNVSQVLKKHPSYRLRENLGTNPRVYYLPVREDGVVED"
     gene            complement(1997..2992)
                     /locus_tag="PLE39_17570"
     CDS             complement(1997..2992)
                     /locus_tag="PLE39_17570"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNE70197.1"
                     /translation="MFSRFEKALIALMFALVFAGVTLVAVSAQDGTPPAAQEENDDCA
                     ECHEDSQMSWQNGPHGNAKSDPIFVSAWTEQGQPGACLTCHVTGYDEATGTWKEDGVT
                     CAACHKDEGGDHPKTSMTVDPTSNTCGNCHTDTRFIAEESWQGSAHYQNGMDCTSCHD
                     PHNASLKITINLKDPNAANDASRLCISCHEEASMNFPYSSHSQQDITCIDCHLEHLEN
                     PNKDIHAVPDHSFKASIKTCSTCHAEQMHAAGNVVSTKEAASVSVTETEPTAEPTPAV
                     EMSSIVPEPTPVSPIGYAGLAALIGLAAGMVLAPWLERWYRLILKKSEEVRHDGK"
     gene            complement(3005..3346)
                     /locus_tag="PLE39_17575"
     CDS             complement(3005..3346)
                     /locus_tag="PLE39_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70198.1"
                     /translation="MNTTLAFVIGVLLRLIVPLTVTALVVFVLHRLDVRWQAEAEKER
                     AALVKDNMPCWKEEGLSVDEIMVRAAKNEQPCWQTHRLSNGYLREACLDCEVFLAAPA
                     PAAKHINAHVS"
     gene            complement(3761..4408)
                     /locus_tag="PLE39_17580"
     CDS             complement(3761..4408)
                     /locus_tag="PLE39_17580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE70199.1"
                     /translation="MTRLLLVDDHAVVRSGLKMLLSGHEEMEIVGEAGSAAEAMREAE
                     RTTPEVILMDIGLPDKTGIEATRDIKKRFPEMKIVALTIHEDEEYFFQMLDAGASGYV
                     PKRAAPDELITAIRAAAAGEVYLYPSMAKLLVHDYLNAERPAEEKLNLDGLTDREREV
                     LTHLAEGAGNDEIAEALVISPKTVERHRENIMRKLNLHSRSELVRYAIRKGIIKA"
     gene            complement(4383..5870)
                     /locus_tag="PLE39_17585"
     CDS             complement(4383..5870)
                     /locus_tag="PLE39_17585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNE70200.1"
                     /translation="MNQDPLIVIYFVYGLAFFSMGLLVAMEGGRSTDARLRMALRPLA
                     GFGLVHAIHEWLEMFKVMGHFNEIAAAVYPVVGLSVLAFSFLSLAAFGAYLVLGSEST
                     WRVSLIIPLALEAIWVYGLLTFKGIYSIDDMYVIADVWTRYSIAIPAGLLAAAGLVVQ
                     QRAFRRAGLVSFGRDSLWASVAFGWYSLVGQIFTAPSHLAPSTFLNSTLFLHLFGFPV
                     QLLRAITAIFASVFVIRFLRAFQVESDHKIAELQEARLHESQNREQLRGELFRKVVGA
                     QEAERQRIARDLHDETGQSLTAIGMGLRGLSDETNEKKRTATLGQLQSLTSDALKELQ
                     RIIADLRPAHLDDLGLSATLRWYASRVHELTSIHVRVDIAGDEPGLDDAVKITIFRIV
                     QEALNNIIKHADATNVNVHLDYHENKIKIFIRDNGRGFDMDAVQSRIGRTSLGLAGME
                     ERAMLLGGVVDVRSRPNYGTEIEAVIPYQLKKVEGSKNDTPVAGG"
     gene            complement(5926..6828)
                     /locus_tag="PLE39_17590"
     CDS             complement(5926..6828)
                     /locus_tag="PLE39_17590"
                     /inference="COORDINATES: protein motif:HMM:NF024645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE70201.1"
                     /translation="MRKFLLLIMLGLLFAFPASVFAQSDLKLTNVNVQLWPEYDQPSM
                     LVITDFEIPATTALPVNVTFRIPQDANLIAVAVYSADGALTNAIFEGPQEDGEWQSFT
                     VSLDSTAGRFEYYQTLSFNGNQRIFSYLWDGIYAVDTFAIRVLEPFDTTSLKTDPALT
                     SISQESNLKYYEGEPVTLASGEQFILNLEYEKTTDTLIVNTQGVQPSAPVDENTPGRV
                     SLSNSLPYIIGGLGVIMIVGGVVYYWQSGRRSTGGKPRKRSRSHAEEQEDGEETYCPQ
                     CGSRSKTGDRFCRTCGARLRHQEE"
BASE COUNT         1731 a         1864 c         1783 g         1483 t
ORIGIN      
        1 acagtttcat actcttcact ggtcaggcgg tgatccacca cccgcaggta tttcacacca
       61 atggagaatg ccagcaggcc aaaggcaatg atgccaagcc cggcagccca ttctacaagc
      121 gatggcgaat aagaggtgta ggagatgacc gcttcaccgg gcaggtaggg aatgaccgca
      181 aggaaaccga cgatgttggt atcgaaacgg aaggcaacca cgccgcccac gatcatcgcc
      241 aggccaagca tccgccagaa gggagataaa cgtgtgggtt tgtagagcag gatgacggtc
      301 ggaatgagcg cgccgaacaa catttcgccg aaccaaaagt tgaaggagag agtccctttg
      361 gtgaggagtt caaatgcctc agtccgcccg ggttcgtagg tgtaggtttg ggcaagccaa
      421 tcccagaagc gccagtacag gtaggcgatc atcatatagc caaggacttg ggaaacgcgc
      481 tcgatcagga tgtcattcac tttggctttg gaggtcaggc gggcggagag catggaggcg
      541 aaaagggtca gtgccacgcc gccaagaatg gctgtgaaca tgaacatcac cgacatctct
      601 ggccgatacc aaatggggcg ggctttgatg acgccataca ctgctcccag cgaagactga
      661 tgcaggcttg aaagtcccag gccgaatatg gcaagcactg gcgcataatg atgcagatgt
      721 tccatcttct cggcaatgcg cgggaatttc ttgcgcagcc agtcaaagga agccacaatg
      781 ggcagcactt ccagcaccag cacggtaaag tacaggccta cacacatggt cacttcccaa
      841 agcagggaat gggtgttcca atagacgaag gatttccaga aacggtccgg gcggccgata
      901 tcgagcagaa gagccatcat agccatgcta tacccggtaa aaccgatgaa agtggctgtg
      961 cgtgcaatgg gttcgtagcg tttgaaacca aagaggtaga cgatggcaca aaggctgaat
     1021 gcgcctgccg ccacagcgat ggaggaaaga tcaattgtga tccacaaacc ccaggggacg
     1081 atgtcggtaa ggttggtgag ggaaagacca aaccagaaga ccgagagacc cgccaccaaa
     1141 gccgcaacca ggaacatacc gagaaagccc agccacaatc cccacggggt aaagatgttt
     1201 tcgcgaatgg ttttcaggat ggttttcatg ttagtcctcc acaacgccgt cttctcgtac
     1261 gggcaggtaa taaacacgtg gattggttcc caggttttcg cgcaggcgat aggatgggtg
     1321 cttcttcaag acctggctga cgtttgaatg cgggtcgttc aggtcaccga agatgcgggc
     1381 gccggttggg caggtgacaa cgcaggccgg ggttgcttcc gcatccacac cagggacaag
     1441 acccacagac atgccgcgat caatgcggtt gtagcagaag gaacatttct ccggtacacc
     1501 gcgtgggcga cgatctactt cgggctcgcc ccattcgggc acggcggggt tcgggtctgt
     1561 gaattccttc caattgaagg cacgggcatc gtacgggcag gccaccatgc agtagcgaca
     1621 gccaatgcat ttgtcgtaat ccatcatgac aatgccatca tcacgatagt aggaggcacc
     1681 cacagggcag acttccacac atggggcatg ttcacactgc atgcatggac ggggcaggta
     1741 cactttttcg ccgttgactt cgcccgcatc aagaactttg gtccactgca attcgggatt
     1801 gatatcatta tgggcgtggc aagccaggtt gcattgttca cagccagtac acttggattg
     1861 gtcgatcacc attacccatt gatgctcacc tttggtggcg ctggaagccc caacttcctc
     1921 gccttgcgca acgcgcatgc cagccgctac tgcgagccca accgcggaaa tttttatgaa
     1981 gtcgcggcgg ttcaaattat tttccgtcat ggcgaacctc ctcagatttc ttcaaaatca
     2041 ggcgatacca gcgctcaagc cacggagcaa ggaccatgcc agccgcaaga ccaatgaggg
     2101 cggcaagacc tgcatacccg atcggagaaa caggggtcgg ctcaggcaca atggaggaca
     2161 tttcaacagc aggggtgggt tcggctgtgg gttctgtttc tgttactgaa acactggccg
     2221 cttccttggt cgaaaccacg ttacccgctg catgcatctg ttctgcgtga caagtgctgc
     2281 aggtcttgat gctggctttg aacgagtgat caggaaccgc gtggatatcc ttgtttgggt
     2341 tttccaaatg ttccaaatga caatcaatac aggtgatatc ctgctgactg tgtgatgagt
     2401 acgggaagtt catgctggct tcttcgtggc agctaatgca caaacgcgaa gcatcattgg
     2461 cggcattcgg gtctttcaaa ttgatcgtga tcttcaaaga agcgttatgc ggatcatggc
     2521 aggaggtgca atccataccg ttctggtaat gggcactgcc ctgccaggat tcttccgcga
     2581 tgaaacgggt atcggtatga caattgccgc aggtgttgga ggttgggtct acggtcatgg
     2641 aggtcttcgg gtggtcaccg ccttcatcct tgtggcaggc cgcgcaggtc acgccatctt
     2701 ccttccaagt gccagtggct tcatcgtagc cagtcacgtg acaggtcagg caggctccgg
     2761 gctgtccttg ttcggtccag gcggatacaa agatcgggtc agatttggcg ttcccatgcg
     2821 gaccgttttg ccaactcatc tgcgaatctt catgacactc ggcacaatca tcattttcct
     2881 cctgggcagc gggaggggta ccatcctgtg ccgaaacagc aacaagggtc acgcccgcaa
     2941 atacaagggc gaacatcagg gcaatcaggg ctttctcaaa acgcgaaaac attttcacat
     3001 ctccttatga cacatgggca ttgatatgct tggcagcagg tgcgggtgca gcgaggaaaa
     3061 cctcgcaatc aaggcaggct tcccgcaaat atccattgga caaacgatgg gtctgccagc
     3121 agggttgttc atttttagcg gcacgcacca tgatctcatc cacagaaaga ccctcttcct
     3181 tccagcatgg catattgtct ttcaccagcg cggcacgttc cttctcagct tctgcctgcc
     3241 agcgcacatc caacctgtga agcacaaaaa cgaccagcgc ggtcacggtc agcggcacga
     3301 tcaagcggag caaaacaccg ataacaaaag caagtgtggt attcatgggg acctcatttc
     3361 agccagtaat tggattctct acgacggagc cattcgccca ccagcatcag caccccaaat
     3421 gggatcacaa ggcggaagac gaagagcaaa aaaatgatct ctgaaccgtt catggcaatc
     3481 tcctttcttt ctcttttctg tatcttgcct acaggatacg cttcccttaa aagaaagtaa
     3541 attcggctcg tcccccatcc tgaacgcagg actcacgcat ctttccccgc ccaaacctgc
     3601 ggactacacc ccacacattc cccaccttta tgggggatct cccccacctt aaatcgacag
     3661 gccacccatg aatggatggc ctgtcggtga attcgacaat tcaggttacg taagcaggat
     3721 tggttttaat tcaaccaaag aaaacggaat ccaacactat ttatgccttg atgatcccct
     3781 tgcgaatcgc ataacgaacc aattcagagc gggagtgtag attcaacttc cgcatgatgt
     3841 tttcacggtg acgttccacc gtcttcgggc tgatgacaag ggcctcggcg atctcatcat
     3901 tccctgcgcc ttcagccaaa tgagtaagca cttcacgttc acggtcggtc aggccgtcaa
     3961 ggttcagttt ttcctcggca gggcgctcgg cattcaggta atcatgtaca agcagtttcg
     4021 ccatggaagg gtacagatac acctcccccg ccgctgccgc acgaatggcg gtaatcaact
     4081 catcaggggc agcccgctta ggcacatagc ctgaagcacc cgcatccagc atttgaaaga
     4141 aatactcctc atcttcgtga atggtcaggg ccacgatctt catttcgggg aatctctttt
     4201 tgatatcacg cgtggcttcg atgcccgttt tatcgggcag accaatatcc ataagaataa
     4261 cctcgggggt tgtccgctcg gcctcccgca tggcctctgc cgcagaaccc gcctcaccga
     4321 caatttccat ctcttcgtgg ccgctcaaca gcattttcag ccccgagcgc accacagcat
     4381 gatcatccac cagcaacagg cgtgtcattt ttactaccct ccaccttctt caattgatag
     4441 ggaatcacag cttcaatctc tgtgccatag ttcggccgcg agcggacatc cacaacaccg
     4501 cccaacagca tggcgcgttc ctccatcccc gccaacccca gggaggtacg cccgatgcgg
     4561 ctttgaaccg catccatatc gaagccgcgc ccattatcgc ggataaatat tttgatcttg
     4621 ttttcgtgat aatccaaatg gacatttaca ttcgtggcat cagcatgctt gatgatattg
     4681 ttcaaggctt cctgcacaat gcggaagatg gtgatcttca cggcatcgtc aagcccgggc
     4741 tcgtcgccag cgatatccac ccgcacgtgg atcgaagtca actcgtggac gcgcgaagcg
     4801 taccatctca aagtggcaga caggcccaaa tcgtcgagat gggcaggccg aaggtccgcg
     4861 atgatgcgct gtaattcctt caacgcatca gaggtgagtg actgtaactg tccaagggtc
     4921 gcagtccgct ttttttcatt ggtttcatct gaaagtccgc gcaggcccat gccaatggct
     4981 gtaagcgatt gacccgtttc atcatgcagg tcacgcgcaa tgcgctgacg ttccgcctcc
     5041 tgggcaccca ccaccttgcg gaataactct ccgcgtaatt gttcacggtt ttgcgattcg
     5101 tgcaaacgcg cttcctgaag ttcggcgatc ttgtgatcgc tttccacttg aaaagcacgt
     5161 aaaaaacgga tgacaaatac agaagcgaaa atagctgtga tggcccgaag tagctgaacg
     5221 ggaaaaccaa agaggtgcag gaacaaggtt gaattcaaaa aagtggatgg cgccagatgg
     5281 ctcggtgcgg taaagatctg ccccacaagg ctgtaccagc caaaagccac cgaggcccac
     5341 aaactatcgc gtccaaaact taccagccca gcacgtcgaa atgcacgttg ctgcacgacc
     5401 agcccggcgg ccgccaacaa ccctgctggg atggcgatgg aataccgtgt ccacacatct
     5461 gcaatgacat acatgtcatc gatggaatag attcctttga aggtcaacag gccataaacc
     5521 cagatggcct ccaatgccag tggaatgatg aggctgaccc gccaggtgga ttcactcccc
     5581 agtacaaggt atgcgccaaa agcagccagt gacaggaacg aaaaagccag caccgataac
     5641 ccaacaaccg ggtaaaccgc cgcagcgatc tcattgaaat ggcccatcac tttgaacatt
     5701 tcgagccatt cgtgtatggc atggaccagc ccaaatcccg ccagcggacg caacgccatg
     5761 cgcaatcgtg cgtcggtgga acgcccgcct tccattgcca ccaacagacc catgctgaaa
     5821 aaagcaaggc catacacaaa gtagatgaca atcaatggat cctggttcat gtgtttttat
     5881 gtataagacc ccaaaggttt ttgaaaaacc ttcagggcct catggtcatt cttcctgatg
     5941 tcgtaatctt gcgccgcaag ttcggcagaa acggtcacct gtttttgaac gggagccgca
     6001 ttgcgggcaa tatgtttctt cgccgtcctc ctgctcttca gcatgagacc gggaacgttt
     6061 gcgcggtttg ccgcccgtgg agcggcgacc cgactgccag taatacacca ccccgcccac
     6121 gatcatgatc acacccaatc cgccaatgat atacggcagc gaattgctca aggaaacgcg
     6181 gccaggggtg ttttcatcca caggcgccga gggttgtaca ccctgcgtat tgacgatcag
     6241 tgtgtcggtc gttttttcat attcgaggtt caagatgaat tgttcgccac tggcaagggt
     6301 tacgggttcc ccttcataat atttgaggtt gctttcctgt gaaatggaag tgagcgctgg
     6361 atcggtcttg agcgaggtcg tatcaaaagg ctccaacaca cgaatggcga aagtatccac
     6421 tgcgtagatc ccatcccaaa gataggagaa gatccgctgg ttgccattga acgaaagcgt
     6481 ttggtaatat tcgaaacgcc ctgcagttga atcaagtgaa acggtgaaag actgccattc
     6541 gccatcttct tgcgggcctt caaatatggc gttggttaga gcaccatctg cagagtaaac
     6601 agccacagcg atcagatttg catcttgggg aatgcggaag gtcacattta cgggcagggc
     6661 cgtagtggcg ggaatttcga aatcggtgat caccagcatg ctgggctggt catattcggg
     6721 ccagagctgc acattcacat tggtcaactt aagatcggat tgggcaaaaa cggaagcggg
     6781 gaatgcaaaa agaagcccca acattatcaa caacagaaac ttgcgcatgg aagaaacctc
     6841 caacaataag agtttctttt a
//
LOCUS       DAOQJC010000171         6833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_6185_length_6833_cov_8.247566, whole genome shotgun sequence.
ACCESSION   DAOQJC010000171 DAOQJC010000000
VERSION     DAOQJC010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6833
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6185_length_6833_cov_8.247566"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..691)
                     /locus_tag="PLE39_17595"
     CDS             complement(86..691)
                     /locus_tag="PLE39_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70202.1"
                     /translation="MSKRRSPFETSNETMPVVAAPPDLYESLRVAEPRKRNRHWEKQH
                     QSYKAVYRGVDPKLSLQVKSIAGDLCVPEGEVARAIIEHALRCYERGDLDLNPHPNPY
                     RMRMTLFPMYDSKPTPSHSKGSKQKPEVLWRVITTWRGFPPELKHELSALASDDGLNV
                     PVGELITALLRFGLKEYQHKRLTLTPVQKRTAFTLSLEGTK"
     gene            complement(701..1471)
                     /locus_tag="PLE39_17600"
     CDS             complement(701..1471)
                     /locus_tag="PLE39_17600"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNE70203.1"
                     /translation="MSTKIICIANQKGGVGKTTTAVSLAHGLVLKGKRVLLIDLDPQG
                     QSATALGRSPEPGVFYLLTMGMTPQETSFIQSWVRFSGREGLHVLPGDQQTMAAQTVL
                     NAQDRPVSAIRQTVSRFFKEGPDYILFDTAPSVGGIQERAVWASDLVIVPTATEFLSA
                     DGVGKVLGMMSILQSKKNWRGNLLGILPTFYDEQTRESKATIEDLKARFANSLLPPIH
                     RSTLLRECAAQGQTIFEMDELCRAAKEYQALAQIVLKY"
     gene            complement(1606..1821)
                     /locus_tag="PLE39_17605"
     CDS             complement(1606..1821)
                     /locus_tag="PLE39_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70204.1"
                     /translation="MTNNPPAEPIEYTPEQRKLLGKAYRLILSWPRDKATETLPAPTG
                     DAKEEASPKDIVHPRDNANARPRKKRR"
     gene            complement(1799..1987)
                     /locus_tag="PLE39_17610"
     CDS             complement(1799..1987)
                     /locus_tag="PLE39_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70205.1"
                     /translation="MARIIVYLRDQDHKALHQLAVREYRVPKAQAALIIRKELERLGL
                     VQIEPEQREETRDKQPAC"
     gene            complement(2104..2685)
                     /locus_tag="PLE39_17615"
     CDS             complement(2104..2685)
                     /locus_tag="PLE39_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70206.1"
                     /translation="MGIVLFSLLSGMHQIVETEFGQGLAMVSVAVATVVILIMILMGV
                     GLGGAIEVAIRKRFLFFKRQPRPGSRLDWFLKLWGSRIDMESTQPTEPVSEGIPPGVS
                     LTDMEELLTLTEQGRRGGKKSLHSDDVQFRAVRDWMILQSRGASITLQQFLEERFGVS
                     AESGMPLVPNQTFYSWRKKFLKELEQHKGKKRD"
     gene            2930..3145
                     /locus_tag="PLE39_17620"
     CDS             2930..3145
                     /locus_tag="PLE39_17620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE70207.1"
                     /translation="MEEWLTTYEAARISNYELDYIRKLIRANKITGRKWGQSWQVNRQ
                     SLLKFMEDREKLGEKRGRKPIHRNNEQ"
     gene            3401..3640
                     /locus_tag="PLE39_17625"
     CDS             3401..3640
                     /locus_tag="PLE39_17625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70208.1"
                     /translation="MFQTHPIPQPQVVHALANLRREWQSAAGESSLLTIRGSVGLILA
                     DLVRELGMPAETQIEILGADLFQEVQEKLDSPERM"
     gene            3726..5396
                     /locus_tag="PLE39_17630"
     CDS             3726..5396
                     /locus_tag="PLE39_17630"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNE70209.1"
                     /translation="MNKRAVIYARVSTDEQTKGYSLQTQVESCKQYAAERGYILLHIF
                     SEDYSGASLDRPELNNVRDFLANEDVDVVIVYDVDRLARKSIYQALIEEEFMRDGAVI
                     EYVIGQYDDSDEGRLQKQIRGSIAEYEKAKILERSKRGKRGKAMSGFVLVGSRPPYGY
                     SVVSEPHKAWFEINEEEAEIVRAVFSWYLHGDESGKPLSMNAIAIKLTNMGVPTRGDK
                     QTHFFKKRDQGVWAAVMIRRILGNETYTGTWYYGKTKMMEDGKKRKPKPKCGFGKQVK
                     RPREEWIAVSVPVIIDEETFKRAGARIKMNAEQSDRSAKHEYLLGRRLRCAKCNYTYK
                     GRTRRQNHYYYCGGKEQVPVSLCDAPAFRGKDVDFTMWNWLVSLIKNPQALIAGLQEK
                     QTNSVEENQVLLDRLAMIENQIADQEIQLGKILDLYLTGDFERGMLTERKLRLETNIV
                     NLRKEHLEISSTIEKLTLTDDQISEIQEFCDGIRDRVETASFDEKRQLLELFDVRGKL
                     AIENNEKVIHATCLLQLQPVSLALTSHSSNTGATATMNCACLSMDPSR"
     gene            5285..6256
                     /gene="mvaD"
                     /locus_tag="PLE39_17635"
     CDS             5285..6256
                     /gene="mvaD"
                     /locus_tag="PLE39_17635"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HNE70210.1"
                     /translation="MSTSTATGVACANIAFIKYWGNRNNELRLPVNGSISMNLDGLFT
                     RTAVSFSASKQDQLSINGAEISGKGLERVSYILDIVRAKAGINNRAEVTSENNFPAGA
                     GIASSAAAFAALALAASKATGLNLSEPELSVLARRGSGSASRSIPGGFVEWQIGREEA
                     DSYSFSIAPADHWNLADCVAIVNASHKKTGSTEGHAIAGTSPLQNARVADASRRLEIC
                     RNAILQRDFEAFANIIEHDSDMMHSVMMTSNPPLMYWQYATVEVFHQVREWRSGGLPV
                     GYTVDAGANVHVLCLGEYKNEVEKRLREIPGVSNVLVAGVGGAAKLV"
     gene            6405..>6833
                     /locus_tag="PLE39_17640"
     CDS             6405..>6833
                     /locus_tag="PLE39_17640"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNE70211.1"
                     /translation="MGLGFVTLIVFFLALGGMIFVHELGHFLAARSVGVEVEEFGFGL
                     PSKKLVTLFTWQGTEFTIHALPLGGFVRPKGENDPNVPGGLASATPWKRLWVLVAGPL
                     MNLITAFVVFSILIAYEGVPVPGSVKIDSVSDNSPAQQAGI"
BASE COUNT         1644 a         1637 c         1904 g         1648 t
ORIGIN      
        1 cgtggacaaa gcgcaggcgg cacgtagtcc gtgccgcctg ccttgaattc gttttcgatt
       61 ttgttttttc gtttttgggg aatgttcatt ttgtgccctc cagggacaat gtgaaggctg
      121 tccgtttttg caccggggtg agggtcaatc tcttatgttg gtattccttc aagccgaaac
      181 gtaacaaggc ggtgatcaat tcaccaacgg gcacattcaa tccgtcatcg gaagccagcg
      241 cggaaagctc gtgtttgagt tcaggcggaa atccgcgcca ggtggtgatc acgcgccaga
      301 gcacttcggg tttttgtttg gaacccttgg agtgtgatgg tgtaggtttg gaatcataca
      361 ttgggaaaag ggtcatgcgc atgcgatagg ggttggggtg tggattgaga tcgagatcac
      421 cacgttcata acaacgcagg gcatgttcaa tgatcgcccg tgccacttca ccttcgggta
      481 cgcaaagatc gccggcgatg gatttgacct gcagggacaa cttgggatcc acaccgcgat
      541 agacggcttt gtagctttga tgttgcttct cccaatggcg gtttctcttt cgcggttcgg
      601 caacccgcag cgactcgtat agatccggtg gcgctgccac gaccggcatg gtctcattgg
      661 aggtttcaaa gggggaacgg cgtttgctca tcctgtcctc ctaatacttc aacacgatct
      721 gtgccagcgc ctgatattcc ttggccgccc ggcacagttc atccatctcg aagatggtct
      781 gtccctgtgc ggcgcattcg cgtaacaggg tcgagcggtg gatgggcggc agtaggctgt
      841 tggcaaagcg cgccttcaga tcctcgatgg tcgccttgct ttccctcgtt tgctcgtcgt
      901 agaaggttgg caggatgccg agcagattgc cgcgccagtt ctttttggat tgcaggatgc
      961 tcatcatgcc cagcaccttg ccgacgccat ccgcggagag gaactcggtc gcggtcggca
     1021 caatcacgag gtccgacgcc cagactgcgc gttcctgaat accacctaca ctcggcgcgg
     1081 tatcgaagag gatatagtcg ggtccttctt taaagaagcg actgaccgtt tgtcggattg
     1141 ccgagaccgg tctgtcctgg gcgttcaaga cggtttgggc ggccatcgtc tgttgatcgc
     1201 ccggtagaac atgcaatccc tcccgaccgg agaagcgcac ccaggattgg atgaagcttg
     1261 tctcttgggg agtcatcccc attgtgagca gatagaagac gcccggttcc ggactgcgcc
     1321 ccaatgcggt ggcagactga ccttgtggat cgaggtcgat caggagcaca cgtttgcctt
     1381 tgaggaccag tccgtgtgcg agcgatacgg cggtggtggt cttgcccaca ccgccttttt
     1441 gattggcaat gcaaatgatt tttgtggaca tatcgtatcc tttgaaaaag gcttgatcta
     1501 ttttggtcct tgctgttgtt tccccagtaa gaaaacagcc actcccatga agagcgggac
     1561 aacgaataag agcaagccaa tgacgaaccc cagaaacacg ggagatcacc ttctcttttt
     1621 tcgcggacgg gcattggcat tatccctggg gtggacaatg tcttttggtg aggcttcctc
     1681 tttggcgtcc ccggtcgggg cgggaagtgt ttccgttgcc ttgtctcgcg gccagctgag
     1741 aatcaggcga taggccttgc ccagcagttt tcgttgttcg ggggtgtatt cgatgggttc
     1801 agcaggcggg ttgtttgtca cgagtttcct cccgctgttc aggttcgatc tgaaccagtc
     1861 ccaggcgctc cagttccttg cgaatgatca aagccgcctg cgcttttgga acacgatatt
     1921 ccctgactgc caattggtgc agggctttgt gatcctgatc ccgcagatat acaatgatgc
     1981 gtgccatagt ctcctttaca cggcgtgatg ggatgaaagc aaaagagccg cgttacggcg
     2041 gctcttttag catctgagtc tatggaaact gtatggtttg aactatgact tttgtacgag
     2101 aattcagtct ctctttttcc ccttgtgttg ttccaattcc ttgaggaatt tcttgcgcca
     2161 gctgtagaag gtttggtttg gcaccagcgg cattccggat tccgccgaca cgccaaagcg
     2221 ctcctccagg aactgctgta atgtaatgga agctccccgg gattgcagga tcatccaatc
     2281 ccgcacggcc cggaattgaa catcatcgga atgaaggctt ttctttccac cccgccgacc
     2341 ttgctcggtc agggtcaata actcttccat atccgtcagg ctgaccccgg gcgggatgcc
     2401 ttccgacaca ggttctgttg gttgggtgga ctccatatcg atccgactgc cccaaagttt
     2461 caggaaccaa tccagcctgc tgcccggtcg gggttgtcgt ttgaaaaaca aaaagcgctt
     2521 ccggattgcc acttcgatgg ccccgccaag tccaaccccc atcaggatca tgatcaaaat
     2581 aaccacagtg gcaacagcga ccgataccat cgccagcccc tgtccgaatt cggtttccac
     2641 gatctgatgc atccccgata acagggagaa caggacaatg cccatacagg taatgaagag
     2701 gatgagaagc ggttttctgg ttgaagattc aggtccgccc ggtgttggaa gttcctgtga
     2761 cataaggcgc ttattttacc tgaaaacaaa atgcccggat tcgtactcca actcataggc
     2821 aagtcataaa gaaagcatag tgccgccgat taaaaggagg tcggcacatt aataaaagaa
     2881 tagcgccggg gcaggatggc acggttataa tcgctgtgga gtaatgaata tggaagaatg
     2941 gttgaccacc tacgaagccg cgcgcatcag caactatgaa ctggattaca tccgcaagtt
     3001 gatccgcgcc aataagatca caggacgcaa gtggggacaa tcctggcagg tcaaccgtca
     3061 atcccttttg aaattcatgg aagaccgcga gaagctggga gagaagcgcg ggcgaaaacc
     3121 cattcaccgt aacaacgaac aatagtgcaa tgtttggggg attgataatt agaatgaatg
     3181 tgctataatt tcattgccag caatgaaatt actggtacaa gaaacgggcc gtgctagtgc
     3241 gccaacacga acacgaccct gacccaaccc accgacgcgg cggagggatg ggctgatcgc
     3301 attatatcag cctgaaaaca tcgccctggt ggagtccatc ggggcgtttt gttttcagcc
     3361 ggatgagaac aggcgctccc aacccaaaag gagtatgccc atgtttcaaa cacaccccat
     3421 cccccaacct caagttgtac atgcacttgc caaccttcgc agggaatggc agtccgcagc
     3481 tggtgagagc agcttgctca ccattcgggg aagcgttgga ctgatcctgg cagacctggt
     3541 tcgtgaactg ggtatgccgg cggaaacgca gatcgagatc ctaggtgctg acctgttcca
     3601 ggaagttcag gaaaagctgg actccccgga gcggatgtag atcggacagg cagctgtgcc
     3661 ggcgcagttg cctgtccctg attgcctttc atttataaaa ccattgaatg ttggagtgat
     3721 acagaatgaa caagcgagcc gttatttatg cacgtgtttc cacagatgaa caaaccaagg
     3781 gctatagcct tcaaacgcag gtcgaatctt gcaagcaata tgcggcagaa cgtggttata
     3841 tcctgctgca tattttttca gaggactaca gtggcgccag cctggaccgt ccggaattga
     3901 acaatgtccg cgactttttg gcaaatgaag atgtggatgt tgtgattgtg tatgacgtgg
     3961 atcgtctggc aaggaaaagc atctatcagg cattgatcga agaggaattt atgcgggatg
     4021 gggcggtcat tgaatatgtc attggtcagt atgatgactc ggatgaaggt cgccttcaaa
     4081 aacagattcg cggaagcatt gcagagtatg aaaaggctaa gatcctcgaa cgtagcaaac
     4141 gaggcaagcg tggcaaggca atgagtggct ttgtgctggt tggatcacga ccaccctatg
     4201 gatacagtgt ggtttcagaa ccgcataaag cctggtttga gatcaatgaa gaggaagcgg
     4261 agatcgtgcg agccgttttt agttggtact tgcatggaga cgaatcaggc aagccgttgt
     4321 ccatgaatgc gatagcaata aaactcacca atatgggtgt gccaacacgc ggtgataaac
     4381 agacccactt cttcaagaaa cgggatcagg gcgtctgggc tgctgtgatg atccgtcgta
     4441 tcctagggaa cgaaacctat accggaactt ggtattacgg caagacaaag atgatggagg
     4501 atggtaagaa acgtaaaccc aagcccaaat gtggatttgg gaaacaagtt aagcgaccgc
     4561 gtgaagaatg gattgctgtt tcagtcccag tcatcattga tgaggaaaca tttaagcggg
     4621 ctggggcaag aataaaaatg aatgctgaac agtctgaccg gagcgcaaag catgaatact
     4681 tgttgggacg ccgacttcgg tgcgccaagt gtaattacac ttataaagga agaacccgtc
     4741 gccaaaacca ttattactat tgtggtggaa aggaacaggt tccagtaagt ttgtgtgacg
     4801 cacctgcttt tcgaggcaag gatgtggatt tcaccatgtg gaattggctt gtaagcctga
     4861 tcaaaaatcc gcaggcgcta atagcagggt tgcaggaaaa acaaactaac tcggtcgagg
     4921 aaaaccaagt cttgttggat cgtcttgcca tgatcgaaaa ccagatcgct gatcaggaaa
     4981 ttcaactcgg gaaaattctt gacctttacc ttaccggcga cttcgaaaga ggtatgctga
     5041 ctgaaagaaa gctgcgtttg gaaaccaata ttgtaaatct cagaaaagaa catttggaga
     5101 tttcgtctac tatagagaag ctgactctca ccgatgacca gatatcggaa atacaggagt
     5161 tctgcgatgg tatccgtgat cgtgttgaaa cggcgagctt tgatgaaaaa cgccagctac
     5221 tagagctatt tgatgttcgt ggtaaactgg caatagaaaa taacgaaaag gtgattcacg
     5281 caacatgtct acttcaactg caaccggtgt cgcttgcgct aacatcgcat tcatcaaata
     5341 ctggggcaac cgcaacaatg aactgcgcct gcctgtcaat ggatccatct cgatgaactt
     5401 ggatgggttg tttacccgca ccgcggtctc cttctctgcc agtaaacagg accaactcag
     5461 cattaatggt gcagagattt ctggcaaagg gttggagcgt gtctcttaca ttcttgatat
     5521 tgtgcgcgct aaggctggga tcaataatcg cgcagaagtg accagcgaga acaacttccc
     5581 tgccggggcg gggattgcct cttcggcggc ggcgtttgca gcgttggccc tggctgccag
     5641 caaagcgacg ggactcaact tgagcgaacc tgaactctcg gtgctggctc ggcgcggatc
     5701 gggttccgct tcacgctcga taccgggtgg ttttgtagaa tggcaaatag ggagggaaga
     5761 agccgattcg tactcttttt ccatcgcgcc agccgaccat tggaatttgg ccgactgcgt
     5821 tgccattgtc aatgcatctc acaagaaaac cggctccacg gagggtcacg ccattgctgg
     5881 gacgagtcca ctgcaaaatg cacgggtggc agatgcttca cggcgactcg aaatttgccg
     5941 caatgcgatt ttgcaaaggg attttgaagc ctttgccaat attatcgagc acgattcaga
     6001 tatgatgcat tcggtgatga tgacttccaa cccgccgttg atgtattggc aatatgccac
     6061 ggtggaggta ttccatcaag tacgcgaatg gcggtctggc ggacttcccg ttggatatac
     6121 tgtagatgcg ggtgcgaatg tccatgtgct gtgtttggga gaatataaaa atgaagttga
     6181 aaaacgactg cgggagatcc ccggcgtgag caatgtgttg gtggctggcg tgggcggtgc
     6241 ggcgaaattg gtttaatttt cggggagaaa aggccccaga aagccgcacc aagtaccggc
     6301 gggggatgaa gcgtaaagtc cgtctaataa aagattaact tcctgcaatc atttgcaaga
     6361 tataatcctt tcgccgcgtc tgtagactcg ggaaaggatt tgaaatggga cttggttttg
     6421 taacactcat cgtctttttt ctcgcgctcg gaggaatgat cttcgtgcac gagttgggac
     6481 atttcctcgc cgctcgttct gtgggggtcg aggtggagga gtttggcttt ggcctgccct
     6541 ccaaaaaact ggtgaccctt tttacctggc agggcacgga attcacgatc cacgcgctgc
     6601 cgctgggtgg gttcgtccgg ccgaaaggtg agaatgaccc gaatgtacca ggcggattgg
     6661 catctgcaac cccttggaag cgtttgtggg tgttggtggc gggcccgttg atgaacctca
     6721 tcacggcctt tgtggttttc tccattctga tcgcatacga aggcgttccg gttccgggct
     6781 cggtgaagat cgattcggtg tcggataatt ctcccgcgca acaggcgggt att
//
LOCUS       DAOQJC010000172         6733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_6302_length_6733_cov_8.216382, whole genome shotgun sequence.
ACCESSION   DAOQJC010000172 DAOQJC010000000
VERSION     DAOQJC010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6302_length_6733_cov_8.216382"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PLE39_17645"
     CDS             <1..591
                     /locus_tag="PLE39_17645"
                     /inference="COORDINATES: protein motif:HMM:NF020318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 family protein"
                     /protein_id="HNE70212.1"
                     /translation="IMTRLQSVADSAHGLPCPLVVMDTAPAAVLGADFDPAVAQRKSK
                     IVCNVGNFHTLAFRLGEMGIEGVFEHHTGEIDLPKLESLLRALADGSLKHEDVFNDMG
                     HGALMYSQSRFEFGKDDFDVVVTGPRRSLFHPSASIPHPAEHLRPYFAVPFGDMMIAG
                     CFGLLAATAEILPELAEPVFGSLRSGRGRGVAPWDG"
     gene            complement(639..2456)
                     /locus_tag="PLE39_17650"
     CDS             complement(639..2456)
                     /locus_tag="PLE39_17650"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNE70213.1"
                     /translation="MKKHLFLLLILPSLACSLLTGGGGNADPANPPSATQEETVDQQP
                     NIPAATPTPFDPLTGSGNSTRTVLTFDELMSGESSSPVDDAAFAIPADAAPPTFTFQG
                     KLELTDEAQAGGFQSLRDDFGYSADPAWMHLPEMNFEFVQSGSHLIPVDQSLVYSNQP
                     TWNYMVAPGRAWNESGDQGFSRASFPFALIERNQNCVHNGVMTFLFNDTTISNVRYQV
                     TQETCMYFKFDMWGQLKAAYTPSQVANAEAVTAAHFEWLKNQLPTKPMSALASDYPNA
                     KVNETFMSSGTTPEHTTVFGVFYNGVNYISNCNTRHGQYAYCSEMRLPSYSTAKSVLA
                     GMAYMHLGQLYDENVGDILVKEYLPEATNWDSITLEHTLDMATGRYEAPTYMTDEDFS
                     AISTNFLVAEKYDDKLKYALSFPASGKPGEQWVYQSTATFVATQTMYAYLRQQAGGDA
                     DLFDMLVQDIFIPLHLSTGTLSSLRTDNSPTGKAFGSHGMFYTQDDIAKIAAFLNSQS
                     GQINGEQILHPDLLAATMQRDSSDHGLSTGSELYNNSFWAKEYRSPCQFYVPFMWGYG
                     GISVAMLPNGATYYAFSDNGEFTFDGAVNELSKLSSVCP"
     gene            2565..3158
                     /locus_tag="PLE39_17655"
     CDS             2565..3158
                     /locus_tag="PLE39_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70214.1"
                     /translation="MMRYFIRAMNILFFVLGVAATVVLFAFFYLAAIRPSTERKWMQI
                     PLPDEALVNVYLSDDGDVLADGENGGLYEFYSDPDPVWKKVSEPEAQHPSVTCGPIME
                     DLYESLPPPGEVSMMVSENCPFAEQANYLEVALLKNGETWFLKSSTNSYVGIGLFVLL
                     PVGVLINVVLYGIWLFFLAIDLILTQKHKRTQTASAP"
     gene            3261..3587
                     /locus_tag="PLE39_17660"
     CDS             3261..3587
                     /locus_tag="PLE39_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70215.1"
                     /translation="MKTLRMINGIFLGLSLCFALAIPILGIGSAAINWNGICYGFTDG
                     QVPCSWWEYTQNEMFWASFIFLPLLVMTLLMWGLMNLIRWVRRGTGTINSPRRFHDKE
                     NSHVQK"
     gene            3574..4053
                     /locus_tag="PLE39_17665"
     CDS             3574..4053
                     /locus_tag="PLE39_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70216.1"
                     /translation="MFKNKAIFLFLALCLTACTSQPALSAEGENWVAFSAEKAQADQM
                     LDWLFTTDAEYWSPTEADIHALESGLPAYLQENKSAFYMTATLIWERLDEYNRQYVGI
                     VLDGRKVIYANYLCQRGEGSGWKEQFAFVADGGACYFQFKFDTSTGGFFDLQVNGEA"
     assembly_gap    4054..4153
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4561..5817
                     /locus_tag="PLE39_17670"
     CDS             4561..5817
                     /locus_tag="PLE39_17670"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNE70217.1"
                     /translation="MNQSFHTENRRKLSAHMADGEVMLFFSGESVRKTADENFPFFTN
                     RNFLYLTGVKQEGSALLLEKKNGIVQERLFAAKPDFAQEIWHGRRFTEIELNQLSGVE
                     RIEDIANLNRALNELLSFQHITALWLCFDALASERSFDIEREFAKGIQGRHPHVVIRN
                     SYPLLAKMRKIKAPEEIEAIRKAMSITEAGIRRLMRISKPGVMEYELEAEFNSELASH
                     GQRRTAFPSILAGGERIFYLHYSNPMAAIADGDLILSDVGAAYDEYCTDISRVFPANG
                     RFSERQAQIYEVALAANRAIMAEVRPGVSFQVLNPICRQASFDGLKALGLLNDIADIG
                     KYVWHGAVHHVGLDTHDVGGYDEPIARDMIFTVDAGIYVREWGIGLRIEDNVLVTADG
                     CENLSAAIPTTLEEIEGFMAGAKAKN"
     gene            5814..>6733
                     /locus_tag="PLE39_17675"
     CDS             5814..>6733
                     /locus_tag="PLE39_17675"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE70218.1"
                     /translation="MITSTRKSDFVQVNGIRLHYLDWGGNGPTLIFLTGMGSSAYIYG
                     GFAPLFTDQFRVLALTRRGHGDSDYPETGYDADTLVEDIRQFMDALQIEKAAFAGHSI
                     AGVELTHFAATHPERVEKLIYLDALDDRRKERVVMEQHPLRNVQIKREESIPPRTLEE
                     YIANVKKEVPEFARIWSTVWDEEFAHAVVVNEEGIVVDKMPASIEKMMVENLIKDYAP
                     KVVKAQIPILSFYGKRKRKLTDDYTEEQKAAYEKFHRDVIEPFFKYLVDEFQGRFPQA
                     QIVVIPDGHHYCFMAQEELVHAEMRKFLLA"
BASE COUNT         1560 a         1531 c         1901 g         1641 t
ORIGIN      
        1 atcatgaccc gcctgcaatc agtggcggat tcggcgcatg gactgccctg tccgctggtg
       61 gtgatggata cagcccccgc cgccgtgctg ggcgcggatt ttgaccctgc cgtggcgcag
      121 cgcaaaagca agatcgtttg caatgtgggg aacttccaca cgctggcgtt ccgtttgggt
      181 gaaatgggaa tcgagggcgt ttttgagcat cacacaggtg aaattgacct gccgaagctc
      241 gagtcacttt tgcgtgcttt ggccgatggc tcgctcaagc atgaagatgt gttcaatgac
      301 atgggtcacg gcgcgttgat gtattcgcag agtcggtttg aatttgggaa ggatgatttc
      361 gatgtagtgg tgacagggcc aaggcgttcc ctttttcatc cttcagcgag catccctcat
      421 cctgcggaac atctgcgccc gtattttgct gtgccgtttg gcgatatgat gatcgcaggg
      481 tgttttggtc tgctggctgc tacagcggag attctgcctg aattggctga acccgttttc
      541 gggtcgttgc gaagcgggcg cgggcgggga gtggctccgt gggacggata atgccgaagg
      601 gttgaggatg cctgcggggc agacatcctc aaaaagaatt aggggcagac agaagagagt
      661 ttggaaagtt cgttgaccgc gccatcgaag gtgaattcgc cgttatcgct gaaggcgtag
      721 taggtggcgc cgttggggag catggcaacg ctgatgccgc catagcccca catgaatggg
      781 acataaaatt ggcaggggga acgatattct tttgcccaga agctgttgtt gtaaagttca
      841 gaccctgtac ttaacccgtg gtcggaggaa tcgcgctgca tggtggcagc gagcaggtct
      901 ggatgcagga tctgttcgcc gttgatctgg cccgattgcg agttgagaaa agcggcgatc
      961 ttggcgatat cgtcctgtgt gtaaaacatg ccgtgactgc caaaggcctt gcctgtgggg
     1021 ctgttatcgg tgcgcagaga agagagtgtg cctgtgctga gatgcagtgg aatgaagata
     1081 tcctgtacga gcatgtcaaa gagatcggca tcaccgcctg cttgttggcg cagataggca
     1141 tacatggttt gggtggcgac gaaggtggcc gtggattgat agacccattg ctcgccaggt
     1201 ttaccgctgg ctgggaaact gagcgcgtat ttcaatttgt cgtcgtattt ttctgcaacg
     1261 aggaaattgg tggagatggc cgagaaatct tcatcggtca tgtaggtggg ggcttcgtaa
     1321 cggccagtgg ccatatcgag tgtgtgttcg agggtgatcg aatcccagtt ggtggcttcg
     1381 gggagatatt ctttgacgag gatgtcaccg acgttctcat cgtacagttg accgaggtgc
     1441 atgtaagcca tgcctgccag caccgatttg gcggtggagt aggatggcag gcgcatttcg
     1501 gaacaatagg catattgtcc gtggcgggtg ttgcagttgg aaatgtagtt cacgccgttg
     1561 tagaacacac caaagacggt ggtgtgttcg ggcgtggtgc ccgatgacat gaaggtttca
     1621 ttgacctttg cgttggggta atcagaagca agcgcgctca tgggtttggt gggcaattga
     1681 ttcttgagcc actcgaaatg cgcggcggtg actgcttcgg catttgcaac ttgtgacggg
     1741 gtgtaagcgg ctttgagctg cccccacatg tcaaacttga aatacatgca ggtttcctga
     1801 gtcacttgat agcggacatt tgaaatggtc gtgtcgttga aaaggaaggt catcacgccg
     1861 ttgtggacgc aattctgatt ccgctcgatg agggcaaatg gaaaagacgc ccgcgaaaaa
     1921 ccctggtcac ctgattcatt ccatgcgcgg ccaggggcca ccatgtaatt ccaggtgggt
     1981 tgattggagt acacaaggct ttggtccacg gggatcaaat ggctgccgct ttgaacgaat
     2041 tcaaaattca tctcgggcag gtgcatccag gcggggtcag cggagtaacc aaagtcatcg
     2101 cgcagagatt ggaatccgcc tgcctgcgct tcgtcggtca attcaagttt gccttgaaag
     2161 gtgaatgtgg gcggtgcagc atcggcgggg atggcgaagg cggcatcatc cacagggctg
     2221 gaagattcgc ccgacatcaa ttcatcaaag gtgaggaccg tgcgggtcga attgccagaa
     2281 ccagtcaacg ggtcgaaggg agtcggggtg gcggcgggga tgttcggctg ctggtcaacc
     2341 gtttcctcct gtgtggcgga gggagggttg gcagggtcag cgtttccgcc gccgcctgtc
     2401 aaaagggaac aagccaggct gggtagaatc aaaagcagga ataaatgttt cttcatttga
     2461 gcctccagta ttaaggatat gtgcctttat agcacaacat ggggcgggca tcatccgcca
     2521 ttggtgggag gtgaatttac agtgtcacgg ctataattca atttatgatg cgatatttca
     2581 tacgggcaat gaacatcctg ttttttgtgc tgggcgttgc ggctacggtg gttttgttcg
     2641 cgttcttcta tctggctgcg attcgtcctt ccacggaacg caagtggatg caaattcccc
     2701 tgcccgatga agcgcttgtc aatgtgtatt tgagcgacga tggcgatgtg cttgcagatg
     2761 gcgaaaacgg cgggctgtat gaattttaca gcgaccccga ccccgtgtgg aaaaaagtaa
     2821 gcgaacctga agcccagcat cccagcgtaa catgcggccc catcatggaa gacctgtacg
     2881 aaagcctgcc gcctcccggt gaagtcagca tgatggtgag cgagaattgc ccctttgccg
     2941 aacaagccaa ttatctggaa gtcgccctgc tcaaaaacgg cgaaacctgg ttcctcaaaa
     3001 gctccaccaa ttcctacgtg ggcatcggtc tttttgtcct tcttccagtt ggcgtgctga
     3061 tcaatgttgt gctgtatggg atctggctgt tctttttggc gattgacctg atcctcactc
     3121 aaaaacacaa gcggacacaa accgcatctg caccttaacc gaaatctgat tcggcgtact
     3181 ggggcagcct ctccccgata cttgatggca attgcatcca tttgggagtc tctgaaaggg
     3241 cgggggaata taatcaattc atgaaaacac ttcgaatgat caacggcatc tttttggggt
     3301 tgagcctgtg ttttgcattg gcgattccca ttttaggaat cggctcggcg gcgatcaact
     3361 ggaatgggat ttgctacggg tttacagacg ggcaggttcc ctgttcctgg tgggagtaca
     3421 cccaaaatga aatgttttgg gcttcgttca tttttctccc cttgctggtg atgacgttgc
     3481 tcatgtgggg tctcatgaat ttgatccgct gggtcagacg cggaacggga acaataaact
     3541 ctccccgtcg ttttcatgac aaggagaatt cccatgttca aaaataaagc aatctttctt
     3601 ttccttgcct tgtgcctcac cgcctgcaca tcacaacctg cgttgagcgc agaaggagaa
     3661 aattgggttg cattttccgc agaaaaggca caagccgacc aaatgctcga ttggctcttc
     3721 accaccgatg ctgaatattg gtcccccact gaagcggata tccatgccct ggagagcggg
     3781 ttacccgcct atttacagga gaataagtcc gcgttttata tgacagcgac tctgatctgg
     3841 gagcgtcttg acgagtacaa ccgtcaatat gttggcattg tccttgacgg aaggaaggtc
     3901 atttatgcca actatttatg tcagcgtgga gaaggaagcg ggtggaagga gcaattcgcc
     3961 ttcgtggctg atggaggtgc ttgttatttt caattcaagt ttgatacgtc cacgggtggg
     4021 ttcttcgatt tgcaagtgaa tggtgaggct taannnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnccaccac agcagccatt gggtattatg ccaaagtggc agagatcttc
     4201 ttgatggtct ttatgggctt tgacatgcaa tgggcattgc agcctgcatc aaaaaatgcg
     4261 gaggctgacc agggttcttg acatcagctg accgctcgca tacaatcagc gagttcgtta
     4321 tcccaccaaa catggaaagg aggtacgcaa gaaaatgaaa accattaact tgttcaaaaa
     4381 attcgtccta agcgcacctc ccgcaaatgg aatctagccg cgatcttgtc ttgttcgctt
     4441 gctttaagtt tccaaccgca gggtgcagct cgccctgcgg tttttgattc tgtcaccttg
     4501 cagggtgatc gtcaaaaaat gaatccacta tgaaggccca aaaggcaaaa tggagaaaaa
     4561 atgaaccaga gttttcacac cgagaacaga cgcaaactat ctgcacacat ggcagatggc
     4621 gaggtcatgt tgttttttag cggcgaaagc gttcgaaaaa ccgccgacga gaacttcccg
     4681 ttttttacaa accgcaactt cctgtactta acgggcgtca agcaggaggg ttctgcatta
     4741 ctgcttgaaa agaagaacgg gatcgttcaa gagcgtttgt ttgcagccaa acccgatttt
     4801 gcacaggaga tctggcatgg caggcggttt acagaaattg aactcaacca actcagcggc
     4861 gtggagcgga tcgaagacat tgccaatttg aacagggcat tgaatgaact tctttccttc
     4921 caacatatca cagccctttg gttgtgtttc gatgcactcg cttcagagcg ttcgtttgac
     4981 atcgaacgcg agtttgcaaa agggattcag ggcaggcatc ctcacgttgt gatcaggaac
     5041 agctatccgc ttttggcgaa gatgcgaaag atcaaggcgc ccgaagagat tgaagccatt
     5101 cgcaaggcca tgagcattac cgaggcggga atccgtcgtt tgatgcgtat ttccaaacct
     5161 ggcgtgatgg aatatgaact tgaagccgaa tttaattccg aacttgcctc acatgggcag
     5221 cgccgcacgg catttccgtc cattcttgca ggcggcgagc ggatctttta tctgcattac
     5281 tccaacccca tggcggccat cgcagacggt gatttgatcc tctcggatgt tggagcggcc
     5341 tacgatgagt actgcacaga tatctcacgc gtttttcctg ccaacggccg tttcagtgag
     5401 cggcaggcgc agatttacga ggtggctctt gcggcaaatc gtgccatcat ggcagaggtg
     5461 cgcccaggcg tttcttttca ggtcttgaat ccgatttgca gacaggcctc tttcgatggg
     5521 ctcaaggctt tgggcttgct gaatgacatt gccgatatcg gtaaatacgt ctggcatgga
     5581 gcggtccatc atgtagggtt ggatacccat gatgtaggcg gatacgacga gccgatcgcc
     5641 agggatatga tcttcacagt ggatgcaggt atctatgtcc gtgagtgggg gatcggtctg
     5701 cgcattgaag ataatgtgct tgtcacggcc gatggctgtg agaacctgtc agcggcgatc
     5761 ccgaccacgc ttgaagagat cgaaggtttt atggcgggcg caaaggcgaa aaattgatca
     5821 cttcaactcg caaaagcgat tttgttcaag tgaacgggat tcgcctgcat tatctggatt
     5881 ggggcggaaa tggcccgacc ttgattttcc tcacaggcat gggaagttcc gcttacatct
     5941 acggcggatt tgctccgctt ttcaccgatc agtttcgtgt tctggccctt acccgtcgcg
     6001 gacatggcga ttcggactac cccgaaacag ggtacgacgc cgatacgctt gtggaagaca
     6061 tccgtcaatt tatggatgcc ttgcagattg aaaaagcagc attcgcagga cattccattg
     6121 ctggcgtgga gcttactcat tttgcagcca cccaccccga acgggtggag aaattgatct
     6181 atctcgatgc actggatgac cgcagaaaag aacgggttgt catggaacag catccgctca
     6241 ggaatgtgca gatcaaaaga gaggaaagca ttccgccgcg taccctcgaa gaatatattg
     6301 ccaacgtaaa aaaggaagtg cctgagttcg ccagaatctg gagcacggtg tgggatgagg
     6361 aattcgctca tgccgtggtg gtgaacgaag aaggaatcgt ggtggataaa atgcctgctt
     6421 ccatcgagaa aatgatggtt gaaaacctca tcaaagacta tgctcccaaa gtggtgaagg
     6481 ctcagatccc gatcctcagt ttttatggaa agcgaaaacg caaactgacc gatgattaca
     6541 ccgaggaaca aaaagccgcc tatgaaaaat tccatcgtga tgtgatcgag ccttttttca
     6601 aatatctggt ggatgagttt caaggcaggt tcccgcaggc gcaaattgtg gtgatccctg
     6661 atgggcatca ttactgcttc atggctcaag aggaactggt ccatgctgaa atgcggaaat
     6721 tcctgcttgc ata
//
LOCUS       DAOQJC010000173         6628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_6466_length_6628_cov_8.284801, whole genome shotgun sequence.
ACCESSION   DAOQJC010000173 DAOQJC010000000
VERSION     DAOQJC010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6466_length_6628_cov_8.284801"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PLE39_17680"
     CDS             <1..642
                     /locus_tag="PLE39_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70219.1"
                     /translation="TVEGVTARLVSADQFQVTLEAGEIPVLVDPHANILRNQFLISPQ
                     FTFVVDARLLKTAPDPLEVNVPLHIGLGPGFSAGENCHAVIETRRSHTLGRVYWSGAT
                     QPDSGQPDGDPRRVLRAPQTGILTAYAEIGDLLQAGQLVADIGEEKITSPFDGVLRGI
                     IRPDIEVLEGMKIGDVDPRSDTSACWLVSDKALSVGGGVLEAVLIKLNEINQN"
     gene            complement(671..2134)
                     /locus_tag="PLE39_17685"
     CDS             complement(671..2134)
                     /locus_tag="PLE39_17685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE70220.1"
                     /translation="MKNIVNSLSLLFHSIRFRLALWFVLILALLLAVFSGLLFYMQIQ
                     ELRAETLNRLQGKLERIESVYVEGGVDPIVLGSLNGGEAPLDRSDILVYFSPNGSVLK
                     MWGPLSKTPVLNLASVEVEEHEPADETITLEGDDGRVEYVFKIIPLFVGQDHTGFMAL
                     GTPLDPRDRLGKLLFTLIVLNGFTLVFALAGGFWLADRAMRPVKTIAAKARSISETNL
                     GQRFNLPQKDEIGQLAGTFDSMLARLESAFARQRQFTADASHELRTPLTIVNLETTRA
                     LSAQRSVKEYQRVLQVIHSENELMSHLVSDLLTLARMDAGREALHREVLDLSDIALEV
                     VERLEPLAARQQVHLFTGELPEVTVLGDRSSLTQMLTNLVENAIKYTAQNGPDLERHV
                     HVEAGTHPAQAQGWVRVSDTGPGISSEHLTHLFDRFYRVDKSRTREEDDEVRPTGSGL
                     GLAIVDGITRLHNGSIEVQSELGKGSTFEVVFPLHLT"
     gene            complement(2145..2837)
                     /locus_tag="PLE39_17690"
     CDS             complement(2145..2837)
                     /locus_tag="PLE39_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE70221.1"
                     /translation="MNPNGGCVRILLVEDNKRLSDSLRLSLMDKGYAVDTAYDGQEGE
                     DLAEFTPYDVIILDVMLPKKDGVEVCRSLRGKRVQSPILMLTARDALEDRVLGLDSGA
                     DDYLVKPFEMDELLARLRALLRRESEDKSGLLAIADLVLDPATHFVKRSGQTIDLTSR
                     EYELLEYLMRNPNRLITRQMAENHLWSYDATVQSNVVDVYIRRLRRKVDDPFETKLIE
                     TVRGSGYRLRRP"
     gene            2955..3320
                     /locus_tag="PLE39_17695"
     CDS             2955..3320
                     /locus_tag="PLE39_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70222.1"
                     /translation="MKKHYTALLAAFLITLCIGAGMALVSGSAMLNQKGLPIADTPAE
                     ATATAEYISAEQAQIAQLQKLVAEYQARELTYQNELADAGQQLEQAKVQIQQYEMILL
                     ALQSRGIISIDPNGRVVLQ"
     gene            3334..3576
                     /locus_tag="PLE39_17700"
     CDS             3334..3576
                     /locus_tag="PLE39_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70223.1"
                     /translation="MKIWKTAIRVWFTLVSLISFLVGWIVLAHSPKPNQFKASDIPAM
                     PKLKPVPSLEEAMFSGRAESLIQINPRTVFLRTSGS"
     gene            3577..4512
                     /locus_tag="PLE39_17705"
     CDS             3577..4512
                     /locus_tag="PLE39_17705"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNE70224.1"
                     /translation="MQADRFRAMNSEIILLAEGDIPQVVLGFIAAREFIETNEKRFTR
                     FSDASELSQLNRSAGTWFNASHEMFEVVKLALEYHHKTSGLFDPSILPNLRAAGYNRS
                     MDEIRRFGTDLQPATQPQTFDSAFASLQLNESRQAILLPPNMQIDLGGIAKGWIAEQA
                     ARLIRQYTSTCAVSAGGDIFLIGYPEGQDVWEIGLEDPRSPAENISVLYAQEGAVATS
                     SVVKRSWKQGEHTRHHLIDPRSGEPANTNWLSVTVFAKHAVAAEAFAKAFLLADETHI
                     RLFEEQNPELTVLAVDASGNLVSLINPERHLNESN"
     gene            4499..5170
                     /locus_tag="PLE39_17710"
     CDS             4499..5170
                     /locus_tag="PLE39_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70225.1"
                     /translation="MNPIEFISNLFAINSLQFWWYLTRAAGLMGYFLIWLSTFWGLVV
                     SSKILDPILHRTFTFDFHEYLSLIGLGFTAVHVIVLLADNYLPFSLWQILIPFLSPYR
                     PFWVGIGIIAFYFTLLVTVTYYFRTQIGLETFRKLHYLSLAAYLGTTLHGLYAGTDSA
                     LPITNLLYIGTALVTLFVLAYWLVMLYLRRQEETQLKKERALAALRSKTQSRLGSARS
                     TKTTH"
     gene            complement(5252..6556)
                     /locus_tag="PLE39_17715"
     CDS             complement(5252..6556)
                     /locus_tag="PLE39_17715"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE70226.1"
                     /translation="MSEYSERPSLLQTYTKSFSPLKNRNLRIYLGGQAISLLGTWMQS
                     TAQSWVVWELTRSESSLGIVVMLNTLPLFLLAPWAGGIADRFNRRMILLVTQGIAMVL
                     AFALAFLIQTGLVQIWHIYTFAFILGIVNALDFPAQQAFIGDLSGMGQVRQAITLNIM
                     GLQVARMLGPAIAGVLLKVVGSAAAFWINGVSFMVVLLSIFMVRSQQASHNRPSARGQ
                     FRETIEFIRSQPRMVDLMVFATLVTFFGLSVFNILPSVADHVLNGDSQTYGLLFASSG
                     AGALISTLVLLPLSQAAKRIGLIVSGAAAWMGTFFFLLSNSTWLPLSMAVIFCISLGA
                     PLVLTTGLGVLQLMSPPDMRARIISLFTMLTFGLQPISSIVIGYSAEHFGTQNAILIN
                     SLCLFAGAALMFFFRDGLRQWELKPAVEPILSSKETSQPASH"
BASE COUNT         1719 a         1768 c         1647 g         1494 t
ORIGIN      
        1 actgtcgaag gggtgacggc gcgtctcgtt tcggcggacc agtttcaggt tacgttggag
       61 gcgggtgaaa ttcccgtgtt ggtcgatccg cacgcaaaca tcctgcgcaa tcaatttttg
      121 ataagtccgc aattcacctt tgtagtggat gcgcgactct tgaaaactgc gcccgatcca
      181 ttggaagtga acgttccgct tcatattgga cttgggcctg gattcagcgc aggtgagaac
      241 tgccacgctg taatagaaac ccgccgcagt cacaccctcg gcagggttta ctggagcgga
      301 gccactcaac ccgatagcgg ccagcctgac ggtgatccgc gccgtgtgtt acgcgccccc
      361 caaacaggga ttttgaccgc atacgcagag atcggtgatt tgcttcaggc agggcagctt
      421 gtggcagata tcggcgagga aaagatcacc agcccattcg acggcgtttt gcgtgggatc
      481 atccgccctg atattgaagt gcttgaaggc atgaagatcg gcgatgttga tccgcgcagt
      541 gataccagtg catgttggct ggtttcagac aaagcgcttt cagtcggcgg gggtgtgctg
      601 gaggcggtgt tgatcaagct caatgaaatc aaccaaaatt aaccctgggt gatttttgct
      661 tgaaggtcct ttatgtcaaa tgcaggggaa aaacgacttc aaaagttgaa ccttttccca
      721 attcgctttg cacttcgatg ctgccattgt gcaaacgggt gatgccgtcc acaatcgcca
      781 gccccaaccc actccctgtt gggcgaactt catcatcctc ttcacgtgtg cgtgatttat
      841 caacgcgata gaaacggtca aacaagtggg tcaggtgttc gcttgagatg cctggacctg
      901 tatctgaaac tcgcacccag ccctgggctt gcgcaggatg cgtgcctgcc tccacatgga
      961 catgtcgttc aagatcgggt ccgttttgcg ccgtgtattt gatcgcattc tcaaccaaat
     1021 tcgtaagcat ctgtgtgagt gaactgcggt cacctaatac ggtcacttct ggcaactcgc
     1081 ctgtaaaaag atgaacctgt tgacgagccg ccaaaggttc aaggcgttca acgacttcaa
     1141 gggcaatatc gctgaggtca agaacttccc tgtgcagggc ttcgcggcct gcatccatac
     1201 gggcaagggt aaggaggtca ctcacaaggt gactcattaa ttcattttcc gaatgaatca
     1261 cctgcaatac gcgttgatat tcctttaccg accgttgagc agacaatgca cgggtggttt
     1321 ccaaatttac gatcgtaagc ggagtccgca gctcgtggct ggcatctgct gtaaactgac
     1381 gctggcgcgc aaaagccgat tcaagccgcg ccagcatgga atcaaacgtc ccagccaatt
     1441 gaccgatctc atccttttgc ggcaaattga aacgctgacc gagattcgtt tcgctgatgg
     1501 aacgggcctt cgcggcaatc gtcttgacag ggcgcatggc gcggtctgcc agccaaaaac
     1561 cgccagccaa cgcaaatacc aatgtaaatc catttaacac gatcagggtg aaaagcaatt
     1621 tacccaaacg gtcacgtggg tcaagcggtg tgcccaaagc cataaaacca gtgtgatcct
     1681 gcccgacaaa aagcggaatg atcttgaata catattcaac gcgcccgtca tcgccttcaa
     1741 gggtgatggt ttcatcggct ggttcgtgtt cctccacttc cacactggca aggttcaaca
     1801 ctggcgtctt tgaaagtggc ccccacattt tcaaaacact tccattgggt gaaaaataaa
     1861 caaggatatc tgagcggtca agcggcgcct cccctccatt aaggctgcct aaaacaattg
     1921 gatccacccc gccctcaaca tagaccgatt cgatccgctc cagttttccc tgcaaacgat
     1981 tcagggtctc ggcgcgcaat tcttgaattt gcatgtagaa caacaatcca ctgaacacgg
     2041 ccagcaacaa cgccagaatt aaaacgaacc acaacgccag cctgaaacgg atgctgtgaa
     2101 aaagcaggga aagggagtta actatatttt tcaaggacaa tccatcaggg tcggcgcagg
     2161 cggtagcccg aaccgcgcac cgtttcgatc aatttcgttt cgaaggggtc atccaccttg
     2221 cggcgcaaac ggcggatata cacatccact acgttagatt gaactgtggc atcgtaactc
     2281 cacaggtgat tctcagccat ttggcgcgtg atcaagcggt tgggattacg catgaggtat
     2341 tcaaggagtt catattcacg cgaagtcagg tcaatggttt gcccgctccg cttgacaaaa
     2401 tgtgtggcag ggtccaacac caggtcagca atagcgagta acccgctttt atcttctgat
     2461 tcgcggcgca gtaaagcccg cagacgagcc agcaactcat ccatctcaaa aggtttgaca
     2521 aggtaatcgt cggcgccgct gtcgagccca agcacacggt cttccagtgc atcgcgggcg
     2581 gtcaacatga gaatcggcga ttgcactcgt ttgccacgca gggaacggca aacttccact
     2641 ccgtcttttt tggggagcat cacatccaga atgatgacat catagggggt aaattccgcc
     2701 aggtcttccc cttcctgtcc gtcgtaggcg gtatccacgg catatccttt atccatcagg
     2761 ctgagccgca gagaatcgct taaccgttta ttgtcttcaa ccaaaagaat ccgcacacat
     2821 cctccgtttg gattcattgt agcatgccaa aatgaccagg ggatgacaaa attccccaaa
     2881 atttgtcatg gctgtgtcat tttcaaatac tatactcgca accacaatca atcggtccac
     2941 ctgcaaggag aagcatgaaa aaacattaca cagccctgtt ggctgcattt ctaatcacac
     3001 tctgcattgg ggcggggatg gcgctcgtaa gcgggagcgc catgttaaat caaaaaggcc
     3061 tgcccattgc agataccccc gctgaagcaa ccgccactgc agaatacatc tctgcagaac
     3121 aggcgcagat cgcccaactt caaaaactgg tggctgagta tcaggcccgt gaattgacct
     3181 accaaaacga actggccgac gcaggtcagc aactcgaaca agccaaagta cagatccagc
     3241 aatatgaaat gatcctgctg gccctgcaaa gccgcggcat tatcagcatc gaccccaatg
     3301 gtcgggttgt tctacagtaa cactggagca tgaatgaaaa tttggaaaac tgccattcgg
     3361 gtttggttca ctctggtttc gctgatcagc ttcctggtcg ggtggatcgt actggctcat
     3421 tcgcccaaac ccaatcaatt caaggcatct gacattcccg ccatgccgaa actcaagcct
     3481 gtcccatccc ttgaagaggc catgttttca gggcgggcgg aaagtttgat tcagatcaac
     3541 ccacgcactg tcttccttcg tacaagcggt tcctaaatgc aagccgatcg atttcgcgca
     3601 atgaattcag agatcatcct tctggcagaa ggtgatattc cacaagtcgt tctggggttt
     3661 attgccgccc gcgaattcat cgaaacaaat gaaaaacgct ttacacgttt ctccgatgca
     3721 agtgaacttt ctcaattgaa tcgctcggca ggaacatggt tcaacgcctc acatgaaatg
     3781 ttcgaggtgg tgaagctggc acttgagtat catcataaaa ccagcggatt attcgaccca
     3841 tcgattctgc caaacttgcg tgctgctgga tacaaccgaa gcatggatga aattcggcgc
     3901 ttcggcaccg accttcaacc tgccactcaa ccccaaacat ttgattccgc ctttgcatct
     3961 ttacaactca atgaaagccg ccaagccatt ctattgccac ccaatatgca aattgaccta
     4021 ggcggcattg ccaaaggctg gatcgccgaa caagccgcaa gattgatccg ccaatacacg
     4081 tccacctgtg cggtcagcgc aggcggtgac atattcctga tcggttatcc agaaggacag
     4141 gacgtgtggg agatcgggct tgaagacccg cgcagccccg ctgaaaacat ctccgttttg
     4201 tacgcgcagg aaggcgctgt ggcaacctct tctgttgtaa aacgatcctg gaaacaggga
     4261 gaacacaccc gtcaccatct gatcgacccg cgcagcggcg agcccgcaaa cacaaactgg
     4321 ttgagcgtca ccgtttttgc aaaacacgca gtagcagcag aagctttcgc caaggcattc
     4381 ctgttggcag atgaaaccca catccgtttg tttgaagagc aaaaccccga gcttaccgtt
     4441 cttgcagtgg atgcaagcgg aaacctcgta agcctgataa atcctgaaag acacttgaat
     4501 gaatccaatt gaatttattt cgaacttatt tgcgatcaac agcctgcaat tttggtggta
     4561 tctcacccgt gccgccgggt tgatgggata ttttctgatc tggctttcca cgttttgggg
     4621 gctggttgtc agcagcaaga tccttgaccc gatcctccat cgaacattca cttttgactt
     4681 ccatgaatat ctttcgttga tcggcctcgg gtttacggct gtgcatgtga tcgttcttct
     4741 ggccgataac tatcttccct tcagcctttg gcagatatta attccctttc tttcgcccta
     4801 tcgacccttc tgggttggga tcggtatcat cgccttctat ttcactttgc ttgtcacggt
     4861 cacctattac ttcagaaccc aaattgggtt ggaaactttt cgcaaactcc attacctcag
     4921 cctcgctgcc taccttggca ccaccctgca tggattatat gcaggcacag attcggcact
     4981 gccgatcacc aatctacttt acataggcac ggcccttgtc accttgttcg tccttgcata
     5041 ctggctggtc atgctttact tgcgcaggca ggaggaaaca caattaaaaa aagagcgggc
     5101 actggccgcc ctgcgcagca aaacgcaatc gcggcttgga tcagcccgat caacgaaaac
     5161 aacacattaa ttttacatag atatgcaaat gcctcttaag caaaaacatc ccgaaaccaa
     5221 tctttcggga tgttttttgc ttaattcaaa actaatgaga tgcaggctgg gatgtttcct
     5281 tggaggatag aatcggctcg acagcgggtt tcaattccca ttggcgcaac ccatcacgga
     5341 agaagaacat gagtgcggct cctgcaaaga ggcaaagcga attgatcaag atggcgtttt
     5401 gcgtgccgaa atgttccgcg ctataaccga tgacgatgga agaaatcggc tgaaggccaa
     5461 aggtgagcat ggtgaacaag ctgatgatcc gtgcgcgcat atcaggcggg gacatgagtt
     5521 gcagcacacc caatcctgtt gtcaacacaa gcggcgcgcc gaggctgata caaaagatga
     5581 ccgccattga aagcggcagc caggtggaat tggacaacaa aaagaaaaaa gtacccatcc
     5641 acgccgccgc accgctgacg atgagtccga tgcgtttggc tgcctgcgaa agcggaagca
     5701 ataccagggt ggagatcaac gcgcccgcac ctgaggaggc aaacagcagt ccataggtct
     5761 gcgagtcgcc attcaagaca tggtcggcaa ccgaggggag aatgttgaag acggagagcc
     5821 cgaaaaaggt cacgagggtg gcgaagacca tcaaatccac catgcgcggc tgggagcgga
     5881 tgaactcgat ggtttcgcgg aattgtccac gcgcactggg gcggttatgg ctcgcctgct
     5941 gagagcggac catgaaaatg ctcagcaaaa ccaccatgaa actcacgcca ttaatccaga
     6001 atgcagcggc tgacccaacc actttcaaca aaacccccgc aatagcaggc cccaacatac
     6061 gcgccacttg caaccccatg atattaagcg taatggcctg ccgcacctgt cccatgccag
     6121 aaaggtcacc aatgaaggcc tgctgggcgg ggaaatccag cgcattgacg atgccaagga
     6181 tgaatgcaaa tgtgtagata tgccagatct gcacaagccc ggtttggatg aggaaggcaa
     6241 gggcaaaagc caacaccatg gcaatgccct gcgtaacaag cagaatcatg cggcggttga
     6301 aacggtctgc gatcccgcca gcccacgggg cgagcaggaa aagcggcagg gtgttgagca
     6361 tcaccacaat gcccaatgaa ctttctgatc gagtcaattc ccacaccacc caactctgcg
     6421 cagtggactg catccacgtg ccaagcaggg aaatggcctg tccaccgagg taaattcgca
     6481 aattacggtt tttcagcgga gaaaagcttt tggtgtaggt ttgcaacaaa gaggggcgtt
     6541 ctgagtattc ggacaaggtt gtattcctta agctgagatt atctttggac gaacagcaag
     6601 gtggaattct tacagggaat gaaggtgt
//
LOCUS       DAOQJC010000174         6565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_6554_length_6565_cov_9.650691, whole genome shotgun sequence.
ACCESSION   DAOQJC010000174 DAOQJC010000000
VERSION     DAOQJC010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6554_length_6565_cov_9.650691"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1822)
                     /locus_tag="PLE39_17720"
     CDS             complement(122..1822)
                     /locus_tag="PLE39_17720"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE70227.1"
                     /translation="MKLKMFTLLVVFGLVLSACSGGGNAAATEPASSGGGTVVLILPE
                     EPTTLNFYLADAAIVRQVADATSMTGLVTIDETGNFVPTLAQEVPTTENGGLSSDYLT
                     VTWKLKEGLKWSDGEALTSDDVKFTVEVLSNPDSGALVGTSGFDLITKVETPDATTTV
                     LTYSEPYPGYLDQFAYGLFPRHATGKPEEMSAWAWNSNPVVAGPFILSNWESGKRLSF
                     TRNPNYYEAGKPYLDEVVFEVVPEPAAQTAMMLNGEAQMQLWPSEFKADYDTLLTGKA
                     QQYLIPGIWNMAIDFNLSAPFDGDPTASAPHPILGDIRVRQAIAHAINYQSLQQDVLK
                     GSVSDSTNPFAYGWYKCELPRQFGYDVEAANKLLDEAGWVMGDDGIRVAKGAKYAEDG
                     TRLSLELQGYTAFDPLQLTEEFIVENLKAVGIEARIQNYDFSIIFGTFEDNSPRAVGD
                     YDMLIFDRGFTTEPQGYNFDAYHSTRIPTAENPTGGNYFRWINAEVDSALDTAGSSFD
                     IQTRKDAYCKVGQAVLDELPQVYLYLFQDNYGIADSLSGYTLNTWGSMSWGIQNWKFK
                     "
     gene            complement(1857..2753)
                     /locus_tag="PLE39_17725"
     CDS             complement(1857..2753)
                     /locus_tag="PLE39_17725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE70228.1"
                     /translation="MSDISLPREQSMGRIVLRRFFKHKLAGIAIGILLILILVAAFAG
                     LNSHNPLEQNPTNSFATPNATNWFGTDELGRDVLSRIMYGGRVSLAVGLLSTLLSILL
                     GITVGALSGYFGGWLDSTLMRVTDAFLTFPTIFVLIILGAFLREQQLFILQNSIFVVI
                     IIIAALSWMWPARLVRGLFLVLREKEFVTASRALGGSDFRIIVQHILPNCMGPILVSG
                     TLQMASAIITESGLSYLGFGVQPPTPTWGSILSTAQNQIFRAPWLGFFPGFMIFITVM
                     AINYIGDGLRDAFDPYVIHSEK"
     gene            complement(2746..3720)
                     /locus_tag="PLE39_17730"
     CDS             complement(2746..3720)
                     /locus_tag="PLE39_17730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE70229.1"
                     /translation="MQTYALRRILQTIPILFIISVLLFFLVRSAPGGPLTAARRNPNI
                     SKEQIEALEEKLGLNDPLPVQYGRWLVDLFQGNLGESIKFRRPVSEMIGERIPNTLLL
                     VGVSFLTTLMLAIPIGILSARKPYSLFDYTMTTITFIGQSIPVYWLGLGLIVVFYVTL
                     KNPFTGDPLFPVGGMNTRGQEGELLDTVWHLVLPVIALSLGWIAWYSRFLRSSMLDVL
                     HEDYVRTARAKGLKDNAVYYKHALQNAILPLVTLIALDLPSLFAGALFVETIFSWPGM
                     GRLFWDAAKGRDYPVLLGVVMITAVLIIVCNILADLAYGWLDPQVKYE"
     gene            complement(3723..5312)
                     /locus_tag="PLE39_17735"
     CDS             complement(3723..5312)
                     /locus_tag="PLE39_17735"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNE70230.1"
                     /translation="MSTKADLVIENAKIFTSNEAKPYAEALAVKGNRIVFVGTNEAAK
                     PYKDESTKVIDGQGRTVTPGFIDSHFHLLWGSIWAGSAQLYEVKDLNDLKTVLQNFAT
                     ENKTAAWVDGRGIKYNIVQNRQQLDEIVPDRPVYINAYDGHTSWANTKALEMAGILQP
                     GVDAPGNGVIVRDENGMATGELRETAMNMVSDLIPEPDEVRKRELLKLTIQRINATGV
                     TSVHNMNGDMEEMMVYAALEDANELTVRVYVPYWVKPETTEEMLKEAAEMANVQGEFA
                     RGGAAKFFMDGVWESYTALNLEPYADDTEAAAEGIYSLEHFTRMAALCDKMGLQIFVH
                     CCGDGAVRRVLDGYEAVQRSNGKRDSRHRIEHIEVIHPDDIPRFTQLGVLPSMQTSHA
                     PFSISEGDVWPTRVGEKRWPLSFAWRNMKNAGNNIAFGSDWPVAPYDPMINLHVGLNR
                     EKWNPSDPDQTLSLEELIIGYTRDAAYAEFKENEKGQIKEGYLADLVLFSHDLFSLPK
                     EEIMTAKPAMTIVDGKIVFEA"
     gene            5399..6469
                     /gene="mnmA"
                     /locus_tag="PLE39_17740"
     CDS             5399..6469
                     /gene="mnmA"
                     /locus_tag="PLE39_17740"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNE70231.1"
                     /translation="MAKEKVVVAMSGGVDSSVAAALLKQQGYDVTGMMLRLWSEPGKE
                     DSNRCCTPDSMAQARRVAGILDIPFYVVDAKEVFRETVVQYFLDGYERGETPNPCLMC
                     NRQIRWTFLLNHALALGAEFMATGHYVRRRSGDDGRTELLRAVDHAKDQSYVLHVLKQ
                     EQLAHALFPVGEYPKPEIRRIAADFGLPTASRADSQDLCFLAGEDYRNFLQRNAAGML
                     KPGNIETPDGRIIGEHTGLANYTIGQRKGLGVASPVPLYVMTKRAASNTLIVGESHEL
                     GSTELTVRDVNWLSGEAPQEPFRAEVKTRYTAKEVTALVFPLEEKQAAVKFDAPVRDI
                     TAGQAAVFYQGEVMLGGGIITQ"
BASE COUNT         1519 a         1693 c         1749 g         1604 t
ORIGIN      
        1 atctggtccg tgaggcaagg cggttgatgg attaagaggc tcacgccttt caacgccgat
       61 aaaaaataag tggtggttga aacaaccgcc acttattttt cttgttattt gccttgcttg
      121 cctacttaaa cttccaattt tgaattcccc aactcatgct gccccaagtg ttgagcgtgt
      181 atccgctcag gctgtctgcg atgccgtagt tatcctggaa caggtacagg tacacttgcg
      241 gcaactcgtc gagtacggcc tgccctactt tgcagtaggc atctttgcgg gtttgaatat
      301 cgaaactgct gcctgcggta tccagtgcgg aatccacttc agcgttgatc cagcggaagt
      361 agttgccgcc tgtgggattt tcggcggtgg gaatgcgtgt ggagtgatag gcatcgaagt
      421 tgtatccctg cggttcagtg gtgaagccgc ggtcaaagat cagcatgtcg taatcaccca
      481 cggcgcgcgg agagttatct tcaaaggtgc cgaagatgat cgagaagtcg tagttctgaa
      541 tccgtgcttc gatacccacg gctttcaagt tttcaacgat gaattcctcg gtgagttgca
      601 gcggatcgaa ggctgtgtac ccttgcagtt cgagcgagag gcgggttcca tcctctgcgt
      661 acttcgcacc ctttgccaca cggataccgt catcacccat gacccagcct gcttcatcca
      721 ataatttatt ggccgcttcc acgtcatagc caaattggcg gggcaattcg cacttgtacc
      781 agccatatgc gaacggattg gtcgaatcgc tgacgctgcc tttcaaaaca tcctgttgga
      841 gtgattggta gttgatggcg tgggcgatcg cctgtcgtac gcgaatatca cccaaaatcg
      901 ggtgcggcgc agaggcggtt gggtcgccat cgaatggcgc gctcaggttg aaatcgatcg
      961 ccatgttcca aataccgggg atgaggtatt gctgggcctt ccctgtcagc agggtgtcgt
     1021 aatcggcttt gaattcgctg ggccacaatt gcatttgagc ctcgccgttc agcatcatcg
     1081 cggtttgggc ggcaggttcg ggcacgacct caaacacaac ttcatcaaga tagggcttgc
     1141 ctgcttcgta ataatttggg ttacgggtaa agctcagccg cttgcccgat tcccaattgc
     1201 tcaagatgaa cggaccagcg accacaggat tgctattcca cgcccaggcc gacatttctt
     1261 cgggtttgcc tgttgcatgg cgcggaaata atccgtaggc aaattgatcg agatagcccg
     1321 ggtagggttc tgaataggtt agaacggtgg tggtggcgtc cggtgtttct actttggtga
     1381 tcaggtcaaa accactcgtg ccgaccaatg cacctgaatc agggttggat aatacctcca
     1441 ccgtgaattt gacgtcatcg gaggtcaatg cttcgccgtc tgaccatttc aaaccttcct
     1501 tgagtttcca ggtcacggtc aggtaatcgc ttgaaaggcc gccattttcg gtggtcggaa
     1561 cttcctgcgc aagggtcgga acaaagttcc ctgtttcatc aatggtaacc aaccctgtca
     1621 ttgaagttgc atccgccacc tgtcgcacga tcgcagcatc tgccagataa aaattcagtg
     1681 tggtcggttc ttcaggcagg atgaggacaa cggttcctcc tccgctggag gcgggttcgg
     1741 ttgcagcggc atttcctccc cccgaacatg cgcttagcac aagcccaaaa acgacgagca
     1801 gggtgaacat ttttagtttc attctctctt ctcctttttt gatttgtttc tattcgctat
     1861 ttttccgaat gaattacata cggatcgaac gcatcgcgca gaccatcgcc gatgtagttg
     1921 atcgccatga ccgtgatgaa gatcataaag cctgggaaaa aacccagcca cggagcgcgg
     1981 aagatctgat tctgcgccgt ggaaaggatg ctgccccaag tgggggtggg gggctgtacc
     2041 ccaaacccca aatagctcaa gcccgattct gtgatgatcg ccgatgccat ttgcagggtg
     2101 ccgctgacca gaatcggccc catgcaattc ggcaaaatat gctgcacaat gatgcggaaa
     2161 tcacttccgc ccaacgcccg tgatgcggtt acgaactcct tttcacgcag caccagaaag
     2221 agaccgcgca caaggcgggc gggccacatc catgaaagcg ccgcaatgat gatgatcacc
     2281 acaaagatgc tgttctgcaa aatgaataac tgctgctccc gtaaaaacgc gccaaggatg
     2341 atcagcacaa agatggtggg gaaggtcaaa aaggcatctg tcacacgcat gagggtggaa
     2401 tcaagccagc cgccaaaata tccactcaac gcgccgacgg taatgcccag caaaatggat
     2461 aacaatgtgg agagcagccc cactgccagt gaaacccttc cgccgtacat gatgcgcgaa
     2521 aggacgtccc gtcccagttc gtcggtgccg aaccagttgg tagcgtttgg tgtggcgaaa
     2581 ctattggtcg ggttttgttc cagcgggttg tgtgaattca accctgcaaa ggctgcaacg
     2641 aggatgagaa tcaacagaat gccgatggca atgccagcca gcttgtgttt gaaaaaacga
     2701 cgcagaacga tgcgtcccat gctttgctcg cgtggaaggg aaatgtcact catatttcac
     2761 ctgcggatca agccagccgt aagccaggtc agcaagaata ttgcagacga tgatcagcac
     2821 agctgtgatc atcaccacgc ccaacagcac ggggtaatcg cgtcccttgg cggcatccca
     2881 gaacaggcgg cccataccgg gccatgagaa gatcgtctcg acaaaaagcg ctcctgcgaa
     2941 caagctgggc agatcgagcg cgatcagggt caccaaaggc aggatggcat tttgcaaagc
     3001 atgtttgtaa taaacagcgt tatccttcaa acccttggct cgtgccgtgc ggacataatc
     3061 ttcgtgcagc acatccagca tgcttgagcg tagaaaacgt gagtaccacg cgatccaacc
     3121 caaactcaat gcgatgacgg gcaaaaccag gtgccagacc gtatcgagta attcaccttc
     3181 ctgtccgcgc gtgttcattc cgccgacagg aaataagggg tcgcctgtga atggattttt
     3241 cagagtcaca taaaacacca cgatcaaccc cagccccagc caatacacgg ggatcgattg
     3301 tccgatgaag gtgatggtgg tcatggtgta atcgaagagc gagtatggct ttcgcgccga
     3361 taatattcca attggaatgg ccagcatcag ggtggtcaga aacgatacac cgacgagtaa
     3421 cagtgtattt ggaatccgtt ctccgatcat ttccgaaacc gggcgtcgga atttgatcga
     3481 ttcgcccagg tttccctgaa acagatcaac cagccagcgc ccatattgaa cgggcaaggg
     3541 atcgttcaag cccaattttt cttccagcgc ttcgatctgt tcctttgaaa tgttcgggtt
     3601 gcggcgcgca gcagtcagcg ggccgcccgg tgcgctgcgc accagaaaga acaaaagcac
     3661 actgatgatg aacaggatcg gaatggtttg caggattcgc cgcaaggcat aggtttgcat
     3721 gattaagcct cgaaaacgat ttttccgtcc acgatggtca ttgcgggttt ggcggtcatg
     3781 atctcttctt tgggcaaact gaatagatca tgtgagaaca aaaccagatc agcgagataa
     3841 ccttccttga tctgcccttt ttcgttttct ttgaactcgg cataggcggc atcacgggtg
     3901 tagccgatga tgagttcttc gagtgaaaga gtttggtcgg ggtcgctggg attccatttt
     3961 tcacggttca atcccacgtg caggttgatc atcgggtcgt agggagccac gggccaatcg
     4021 ctgccgaatg ctatgttgtt gcctgcattt ttcatgttgc gccacgcgaa cgaaagcggc
     4081 cagcgttttt cgccaacgcg ggttggccag acatcacctt cggagatgct gaacggagca
     4141 tggctggtct gcatggatgg cagcacgcca agttgtgtga agcgcggaat atcatcggga
     4201 tggatgacct cgatatgttc aatgcgatga cggctatccc gcttcccatt cgacctttga
     4261 actgcttcgt acccatccag cactcgtctc accgcaccat ccccgcagca atggacgaag
     4321 atctgcagac ccattttgtc gcacagggct gccatgcggg tgaaatgctc cagtgagtaa
     4381 ataccttccg ctgctgcttc ggtgtcatcg gcgtatggtt ccagattcaa agccgtgtac
     4441 gattcccaga ccccatccat gaagaatttt gcagccccgc cgcgggcaaa ttctccctgt
     4501 acgttcgcca tctcggcggc ttctttcaac atctcttcag tggtttcagg tttgacccag
     4561 tatggcacat acacacgcac ggtcaattca ttcgcatctt caagcgcggc atagaccatc
     4621 atttcttcca tgtcgccgtt catgttgtgg acgctggtga cgcctgtggc gttgatgcgc
     4681 tgaatggtca atttgagcaa ttcgcgcttg cggacttcat ctggttcggg aatcagatcg
     4741 gagaccatgt tcatggcggt ttcgcgcaat tcgcctgtcg ccatcccgtt ctcatcacgg
     4801 acgatcacgc cattaccggg tgcatccaca ccgggttgaa ggattcccgc catttccaag
     4861 gctttcgtgt ttgcccatga ggtatgccca tcgtaggcat tgatatacac tgggcggtct
     4921 ggaacaattt catccagttg ttggcggttt tgcacaatat tgtatttaat gccgcgtcca
     4981 tccacccagg cggcggtctt gttttcagtt gcgaagtttt gaagaacggt cttcaggtcg
     5041 ttcaggtctt tcacttcgta aagctgggcg ctgcctgccc aaatggatcc ccacaataaa
     5101 tgaaaatgcg agtcgataaa ccccggggta acggtgcgtc cctgcccatc gatgactttt
     5161 gtggattcat ctttatatgg ttttgccgct tcgttcgtgc cgacaaaaac gatccgattg
     5221 ccttttacag ccagcgcttc cgcatacggc ttggcttcat tgctggtgaa gatttttgcg
     5281 ttctcgataa ccagatccgc cttggtagac attgcttctc tccttattgc ttcgatataa
     5341 atgcttcgaa tatacattaa ctaagggggt gatgcaagtg gtttataatc acgccattat
     5401 ggctaaggaa aaagttgttg tcgccatgtc tggcggcgtg gattcttcag tggcggcggc
     5461 cctgctaaag cagcagggat atgacgtgac agggatgatg ctgcgtttat ggtctgaacc
     5521 tggcaaggaa gattcaaacc gctgttgtac acccgattcg atggcgcagg cacggcgggt
     5581 ggctggaatt cttgatattc ccttttatgt ggtggatgcg aaggaagtct tccgcgagac
     5641 ggtggtgcaa tattttcttg atggctacga gcgcggcgaa acccccaatc catgcctgat
     5701 gtgcaatcgc cagatccgct ggactttttt gctcaatcat gcgctggccc tgggcgctga
     5761 attcatggca acagggcact acgtccgacg caggagcggg gatgacggac ggacggaatt
     5821 actccgcgct gtggaccatg ccaaggatca atcctatgtt ttgcatgtct tgaaacagga
     5881 gcagcttgcg catgcgcttt tcccagtggg tgagtatccc aagcctgaga ttcgccgcat
     5941 cgctgccgac tttggcctgc ccactgcttc gagagcagac tctcaggatt tgtgttttct
     6001 ggcaggtgaa gactatcgta actttttaca gcgaaatgcg gctgggatgt tgaaaccagg
     6061 caatattgaa accccagacg gcaggatcat cggtgagcat actggattgg ctaattacac
     6121 catcggtcag cgtaagggat tgggcgttgc ctctcctgtg ccgttgtatg tgatgacaaa
     6181 acgtgccgcg agcaacacac tgatcgtcgg tgaatctcat gagttgggtt ccactgaatt
     6241 gaccgtacgt gatgtcaact ggttgagcgg tgaagcgccg caggagccct tccgcgcaga
     6301 ggtaaaaacg cgctacacgg ccaaagaggt cacggcgctg gttttcccat tggaagagaa
     6361 gcaagccgca gttaagtttg atgcgcctgt tcgagatatc acagcaggtc aggcggccgt
     6421 gttttatcaa ggtgaggtga tgcttggagg cggaatcatc acccagtaag tttttgcgcg
     6481 tagccagaat aaagcaaaca agaaacaagc aatttttgcc cttgatcgta accttcaacc
     6541 ttcaaccttc aaccttcaac cttca
//
LOCUS       DAOQJC010000175         6549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_6579_length_6549_cov_11.429165, whole genome shotgun sequence.
ACCESSION   DAOQJC010000175 DAOQJC010000000
VERSION     DAOQJC010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6579_length_6549_cov_11.429165"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    2..236
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            398..2206
                     /gene="thrS"
                     /locus_tag="PLE39_17745"
     CDS             398..2206
                     /gene="thrS"
                     /locus_tag="PLE39_17745"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNE70232.1"
                     /translation="MSQITERYEDSQLYRIRHSAAHVMAQAVLEMFPDGKVTIGPPVE
                     NGFYYDFDLPRNLTPEDLEAIEKRMRQIVQGKHEFRKTVISAEEAKKIFADQPYKLEL
                     IEGLEKGGLDEYGNPLAEKPDISIYQHDTFTDLCRGPHVANTKEIKQDAFKLMSIAGA
                     YWRGDENNKMLQRVYGTAWESKDQLKEYLQQLEEAKKRDHRKLGKELEIFIFDDEVGP
                     GLPLWLPNGGVLIEELENLAKEMELKAGYDRVRTPNVTKEDLFLRSGHLPYYAESMYP
                     PMELEGVKYYIKPMNCPMHHKIFGSKGRSYRELPVRLAEYGTCYRYEKSGELFGLMRV
                     RSMQMNDAHIYCSEEQFEQEFMGVVELYKKYFDLFGIEKYVMRLSLHSKAGLGKKYVN
                     NEALWIKTEDMVRRAMNNGGVPYVEREDEAAFYGPKIDVQIWSAIGREFSLATNQVDF
                     AVPERFDLKFTNKEGHDEVPLCIHRAPLSTHERMVGFLLEHYAGNFPVWLSPEQVRVI
                     SITDGQNEYVDKIARELRENGIRASADVSSQRMNAKIRQAQLMKVPYMIVVGDNEMQA
                     NQVSLRARDGSQQNNISLGEFIARAKDRISRRAKEL"
     gene            complement(2272..4515)
                     /locus_tag="PLE39_17750"
     CDS             complement(2272..4515)
                     /locus_tag="PLE39_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70233.1"
                     /translation="MKNNRPFNLLDDIAKDHIPDSTNLTPHLAARLERNSRMFTFRTH
                     RLVAMLIALIILIALSGVAYAIGRSLGYIPGVGIVDQSTPIRILAEPVTQTRDGITIT
                     VSGVVLSSSKTVVLYKVENIPQEKLSQDMAFPGCGSKPEEHTFLKTVEDPMPRDLGYE
                     MNGWGTGYEVRHIFAALPAEINDAVLVIPCIERSLSGTLPENWELPLHFIPAPPEMSV
                     APVIEVTPSQEPVEGSPLKLEKIAETEKGYILVGTFDSTGLPQGAQAMDFSTFPIFTD
                     ANGQEVIYDFANFQLDQPAEFPPIGTFPWAFEIIGKDHAYPLTMTVSAVAAQYSGSSA
                     SFEFDAGANPQEGQVWDLNQDVELVGFPVRVVKATRTVEGYVFNFESSAFFHGVSLTM
                     GEGSLPGGTGMDDPNHFKAVVRFNGAIPSGKMEVLITNPVMAISGNWNIQWQPEISST
                     ESVSTPTTADQTCLTRDSWQAALDNYQPVPPELNGRLLVYGALSDDWLNYDNYGSFMS
                     RLDRSDKQIISQGVFPSLSPDGKFVAFAWGEGLNIAEIASGAQFHIPNTTSDDNFPVW
                     SPNGAKIAFISGTDLNLYIINPDGSERTRVADLNGDEQLIDWTNDGTGLFYGVSVEGG
                     ISLRKIDLVSGAVAELFTIPQHRTYEDVAISPDGTHIAYHARENLQDAIYISNLDGSE
                     RRLFAQMGGWIVGAPFWSPDGNWLIVIIRSTDLPDRPTEMALVNVNDCRIHPFPLSVE
                     MVRGWMP"
     gene            complement(4518..5063)
                     /locus_tag="PLE39_17755"
     CDS             complement(4518..5063)
                     /locus_tag="PLE39_17755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNE70234.1"
                     /translation="MDEVTAIRRIKMGDFGGLEYLMSLYQVKAARAAFLITCDKAKAE
                     DVVQEVFLRIFQRPGSFDENRPFEPYLMRSVVNAALNASRGEKRFISLEANAETIERL
                     ILQEASVESQAEYSQLKSEILNALGQLPPRQRAAIVQRYYLDMSEKEMAFTLDAAPGT
                     VKWLLNSARNTLRDLLKKERI"
     gene            complement(5230..>6549)
                     /locus_tag="PLE39_17760"
     CDS             complement(5230..>6549)
                     /locus_tag="PLE39_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70235.1"
                     /translation="VPPLTIKVTRMSTYPAAGDTPIEYEFDAGENPKVGDDWQVNKEI
                     QAAGYSFTLVSVRADTDQDMPGMYGYRFSFKPLADPISNLYLELKGQDFMGYTQNGNI
                     DGFTYFVGYPELPKGKLTVSFSLEINDPQAWTVQWTPDDSSANIVTPTEAPQACLTTD
                     SWEAALANPAPIPSDLTGKLVAYGRTVDDGKDPNPENTGIFTINLDGSNKQAFGQGMS
                     PMLSPDGSQVVYFDNAGLHLMDTASGVTSHIPNTVEGDIFPLWSPDGTQIAFFHGLDS
                     DLYIIQPDGSGLRRALQGIDAEQLVGWTSDSRGLYYYVNTETGSELRSVDIESEGVTS
                     LFEHDGYAFFNASPDGSKIAFVGHPNNLYISNTDGTGRELLGFMGNLFISTPLAWSPD
                     GKWVIVTIRRLEDPDIAGLPIPHALVSVTNCQIIPFSWSGDVYNWVP"
BASE COUNT         1534 a         1580 c         1878 g         1557 t
ORIGIN      
        1 atcatacgcg ttgaccaaga cgagtacgtg accattgttc tgacagagag aagaagccag
       61 agactgagag cctcttccag aagcaatcac cgaataccac ttgcgagcgt gaccttgaaa
      121 tcgaaagaga gtaagggaaa cgggttgctc cgttagaggc ttaagagtgg cgtgttgggg
      181 aaacctcaac atgcaattcg ggtggaacca cgtgagatta aactcccgtc ccgttgtgga
      241 tgggagtttt ttattttagg aggtcgccat gaccacatat actcgttggc ggcccggagg
      301 tttgtgactg ctcacctgcg ctacggtgca agtgtttctc cggggcgcga aatgttcatg
      361 aagcaggatc aagccaaagt taagaaagga agcgaaaatg tctcaaatta cagaacgtta
      421 tgaagattca caactgtatc ggatccgtca ttctgcggcg catgtgatgg cgcaggcggt
      481 gttggaaatg ttccccgatg gcaaggtgac gattggcccg ccggtggaga atgggtttta
      541 ttatgatttc gatctgccgc gcaacctgac gcccgaagat ctggaagcca tcgaaaaacg
      601 gatgcgccag atcgtgcagg gaaaacacga gttcaggaaa acggtcatct ctgcggagga
      661 ggcgaagaag atctttgccg accagccgta taaacttgag ttgatcgagg gtcttgaaaa
      721 aggcggtttg gacgaatacg gcaacccgct ggccgagaag ccggatattt cgatctacca
      781 gcacgatacg tttacggacc tgtgccgcgg tccgcatgtg gcgaacacga aagagatcaa
      841 gcaggatgcc tttaagttga tgtcgatcgc gggtgcatac tggcgcggtg atgaaaacaa
      901 taagatgctc cagcgcgtgt acggtacggc ctgggagagc aaggatcagt tgaaggaata
      961 ccttcaacag cttgaggaag caaagaagcg tgatcatcgc aagctgggca aggaactgga
     1021 aatcttcatc tttgatgatg aggtggggcc cggcctgcct ttgtggctgc ccaatggcgg
     1081 tgtgttgatc gaggaattgg aaaacctcgc caaggaaatg gaactcaagg ctggctacga
     1141 ccgtgtgcgc acgccgaatg tgacgaagga agatctcttc ctgcgttcgg ggcatctgcc
     1201 ctattatgcc gagagcatgt atccgccgat ggaattggaa ggcgtgaagt attacatcaa
     1261 accgatgaac tgcccgatgc accacaagat tttcggttca aagggacgtt cataccgtga
     1321 gttgcctgtg cgtctggcgg agtatggcac gtgttatcgc tacgagaaat cgggcgagtt
     1381 gttcggcctg atgcgtgtgc ggtcgatgca gatgaacgac gcccacattt actgctctga
     1441 ggaacaattt gagcaggagt tcatgggtgt tgtggaactg tataaaaagt atttcgatct
     1501 gttcggcatc gaaaagtatg tgatgcgact ctccctgcac agcaaggcgg ggctgggtaa
     1561 gaaatatgtg aacaacgaag cgttgtggat caagacggaa gacatggtgc gccgcgccat
     1621 gaataacggc ggcgttccat acgtggagcg tgaagacgaa gccgccttct atggccctaa
     1681 gatcgatgtg cagatttggt cggcgatcgg gcgtgaattt tcgctcgcga cgaaccaggt
     1741 ggattttgcc gtgcccgaac gttttgacct gaaattcacg aataaggaag gtcatgacga
     1801 ggtgccgctt tgcattcatc gcgctccgct ttccacgcat gagcgcatgg tgggtttcct
     1861 gttggagcat tacgcgggca acttccctgt gtggctttcg ccggaacagg tgcgcgtgat
     1921 ctcgatcacg gacggtcaga atgagtacgt ggataagatc gcaagagagt tgcgcgagaa
     1981 cggaatccgc gccagtgccg atgtttcatc gcagcgcatg aatgccaaga tccgtcaggc
     2041 gcagttgatg aaggtgccgt acatgatcgt ggtcggcgat aacgaaatgc aggcgaatca
     2101 ggtttcgctg cgcgcacgtg atggcagcca gcagaataat atttcattgg gcgagtttat
     2161 tgcgcgggcg aaggatcgaa tttcaagaag ggcgaaagag ttgtaaaaga aagaagaaag
     2221 agacaggcgg aggattttcc tccgcctgtt tttttaggag catttgtggg tctatggcat
     2281 ccagccgcgg accatttcca cgctgagggg gaaggggtgg atgcggcaat cgtttacgtt
     2341 gaccagcgcc atttcggtgg ggcggtcggg caggtcggtg gagcggataa tgacgatcag
     2401 ccaattccca tccggtgacc agaatggcgc gccaacgatc cagcctccca tttgtgcgaa
     2461 cagtctgcgt tccgagccat ccaggttgga gatgtaaatt gcatcctgca ggttttcacg
     2521 cgcatgatat gcaatgtggg tgccatcggg agaaattgca acatcttcgt atgtgcggtg
     2581 ttgaggaatg gtgaaaagtt cggccactgc tccactgacg agatcaattt ttcgcaggga
     2641 aataccgccc tccacgctca caccatagaa caagccggtg ccatcgtttg tccaatctat
     2701 caattgctca tcaccattca aatcggctac ccgtgtccgc tcgctgccat cgggattgat
     2761 gatatataga ttgaggtcgg ttccgctgat gaaggcgatc tttgccccat tcggcgacca
     2821 gaccgggaaa ttatcgtcag aggtggtgtt gggaatgtgg aattgcgcac cgctggcgat
     2881 ttcggcaatg ttcaggcctt caccccatgc aaaagccacg aatttcccat ccggtgaaag
     2941 cgaggggaaa acgccctgcg agatgatctg cttgtcactg cggtcaaggc gcgacatgaa
     3001 actaccatag ttatcgtagt tgagccaatc gtcactgagg gctccataga ccagcagcct
     3061 gccgttcagt tcggggggaa ctggttggta gttatccaat gccgcttgcc acgaatcccg
     3121 cgtcaggcag gtttgatccg ctgttgtggg tgtggaaact gattcggtgg aggaaatctc
     3181 cggttgccat tgaatgttcc agtttccgga gatcgccatc acggggttgg taatgagcac
     3241 ttccattttt cctgaaggga tggcaccgtt gaaccgcacc acggctttga aatgattcgg
     3301 gtcatccatg cctgtgccgc cgggcaatga accttcaccc attgtcaggc tgacgccatg
     3361 gaaaaaggca gaactctcga agttgaagac atagccttct acagtgcggg ttgctttgac
     3421 gactcgcacc gggaatccga ccaactcaac atcttgattc agatcccaaa cctgcccttc
     3481 ctgtggattt gcccccgcat caaactcgaa gctggcgctt gatcctgaat attgggcagc
     3541 cactgcgctg acggtcatgg tcagcgggta ggcgtggtct ttgccaatga tttcaaaagc
     3601 ccacgggaag gtgccgattg gtggaaattc agcaggctgg tcaagctgga aattggcaaa
     3661 atcataaatg acctcctgtc cattggcatc ggtgaaaatg gggaaggtgg agaaatccat
     3721 ggcttgagcg ccctgcggca ggccagtcga atcaaaggtg ccaacaagaa tatagccttt
     3781 ttctgtttcc gcaatttttt ccagttttag cggggatcct tcgacaggtt cctgcgaagg
     3841 tgtcacctcg atcactgggg caacgctcat ctccggcggt gcagggatga agtgcaaggg
     3901 cagttcccaa ttttcaggca atgttcccga gagcgacctt tcgatgcatg gaatgacaag
     3961 cacggcatca ttgatctctg cggggagtgc ggcaaagatg tggcgaactt cataccccgt
     4021 gccccagcca ttcatttcat agcccaggtc ccttggcatg ggatcttcca cagttttcag
     4081 gaatgtgtgc tcttcaggtt tcgagccgca accggggaag gccatgtcct gcgaaagctt
     4141 ttcctgtgga atgttttcca ccttgtaaag caccacggtc ttgcttgagc tcaacaccac
     4201 gccgctgact gtgatcgtga ttccatcccg agtttgtgta acaggctcag ccagtatgcg
     4261 gatgggtgtg ctttgatcca cgatccccac accgggaatg tagccaagag aacgcccgat
     4321 ggcataggct actccgctta aggcgattaa gataatcaat gcaatcagca tcgccacaag
     4381 cctgtgggtg cgaaatgtaa acatccggga attcctttcg agacgggctg caaggtgcgg
     4441 cgtgaggttt gtgctgtcag ggatgtggtc ttttgcgata tcatccagca agttgaaagg
     4501 tctgttgttt ttcattttca gatcctttcc ttcttgagca ggtcacggag ggtgtttcgg
     4561 gcggagttca aaagccattt gacagtgccg ggggcggcgt caagtgtgaa tgccatttcc
     4621 ttttcgctca tatcgaggta atacctttgc acaatggctg cacgttgccg tggcgggagt
     4681 tgccccagtg cattcaatat ctcgcttttg agttgtgaat attccgcctg agattcaacc
     4741 gaagcctctt gcaggatcag tcgctcgatg gtttctgcat tggcttcaag cgagataaaa
     4801 cgtttttcgc cgcggctggc attcaatgca gcgttaacca cactccgcat tagatagggt
     4861 tcgaaaggac ggttttcgtc aaaactgcct ggacgctgaa agatgcgcag gaagacctcc
     4921 tgaacgacat cctccgcttt tgctttatcg catgtgataa gaaacgcggc gcgcgccgcc
     4981 tttacttgat acaacgacat taaatactcc aacccgccaa aatcacccat ctttatgcgg
     5041 cggatggctg taacttcatc catacaattc tccgtgttgt tgagcgctca tactttaatt
     5101 acacacgaca gctcaaaaag gttggcagga aattcctcaa ataggaaatg caatttccat
     5161 agagtttgaa ttaaaacaca caggcactcg gttatccgaa tgcctgtgat cgtttcattt
     5221 gttgaattgt tatggtaccc agttgtagac atccccgctc catgaaaagg ggatgatctg
     5281 gcagtttgtc acgctaacca acgcgtgcgg aattggtagc ccggcgatat cgggatcttc
     5341 cagtctgcgg atggttacga ttacccactt gccatcgggt gaccaggcca aaggtgtgga
     5401 aatgaatagg ttgcccatga acccaagcaa ctcgcggcct gtgccgtccg tgttggaaat
     5461 gtacaggttg ttggggtggc caacaaaggc gatcttactg ccatcgggcg aagcattgaa
     5521 gaaagcgtaa ccgtcatgtt caaacaggct ggtcacgccc tcggattcaa tatccacact
     5581 gcggagttcc gagcctgtct cggtgttgac gtagtaatac aagccgcggc tgtcggatgt
     5641 ccaaccgact aattgctcgg catcaatgcc ctgcagggcg cggcggagtc cgcttccatc
     5701 gggctggatg atgtacaaat cagagtcgag gccgtggaag aaggcaatct gagttccatc
     5761 gggcgaccag agcgggaaga tatcgccctc gaccgtgttg gggatatggc tcgtcacacc
     5821 gctggcggta tccatcaagt gcaaacctgc gttgtcaaag taaaccacct gcgaaccatc
     5881 tggtgagagc atgggactca tgccctgacc gaaagcctgc ttgttgcttc cgtcaaggtt
     5941 gatggtgaat atgcctgtgt tttcagggtt cgggtctttg ccatcgtcca cggtgcggcc
     6001 gtaggcgacg agtttccctg tcaggtcgga gggaatcggt gcggggttgg ctaatgcggc
     6061 ttcccacgaa tccgttgtca ggcaggcttg aggcgcttcc gttggggtga cgatgttcgc
     6121 ggaagagtcg tcaggtgtcc attgcacagt ccatgcctgc ggatcgttga tctccaggct
     6181 gaatgaaacg gtcagtttac ctttcggcaa ctcgggatag cctacgaagt acgtaaagcc
     6241 gtctatgttg ccgttttggg tgtagcccat gaaatcttga cctttgagtt ccaggtagag
     6301 attggagatt gggtctgcca agggcttgaa gctgaaacga tagccgtaca tgcctggcat
     6361 atcttgatcc gtgtccgcgc ggacggatac cagcgtgaac gaatacccag cggcctgaat
     6421 ttctttgttg acctgccaat catcaccgac ctttggattt tcgcccgcgt caaattcgta
     6481 ttcgatcggc gtgtcacccg cggcgggata cgtggacatc cttgtgacct tgatggtcag
     6541 cggcgggac
//
LOCUS       DAOQJC010000176         6362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_6873_length_6362_cov_9.351673, whole genome shotgun sequence.
ACCESSION   DAOQJC010000176 DAOQJC010000000
VERSION     DAOQJC010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6873_length_6362_cov_9.351673"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /gene="rimO"
                     /locus_tag="PLE39_17765"
     CDS             <1..1298
                     /gene="rimO"
                     /locus_tag="PLE39_17765"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNE70236.1"
                     /translation="DSMAQLLIRDGYRAMDEPDKAAVLIVNTCGFIGPAKEESYRVLG
                     ELAKSKHSGQVLIAAGCLTQRYGVEVAQKVQGIDGILGTRRWMDIVQVVKEARKGKHP
                     EPIYHLPEAATVGVDEQDALRVSVAGASAYLKVADGCRRPCAFCAIPLIKGTAVSRPV
                     DAIVNEARTLRDNGVRELVLIAQDTTDYGHDLGMKDGLAVLLEQLTDSVPDMDWIRVM
                     YAYPGYVTDRLIEVMATRKQILPYLDMPLQHAHPKTLYRMKRPSNIEWVHKTLGKMRA
                     AIPELSIRTTFIVGYPGETDEEYQALYDFVKEIRFDRVGAFQFSFEPGTTSAPLGDPV
                     PASVKQERYEHLMELQQTISLQVNQSYVGKTLDVLVEGFDNGISIGRSYRDAPEIDGM
                     VFIEGKANVGEIVPVRISGAMAYDLTGVLAQSQQQVIKL"
     gene            complement(1386..1892)
                     /locus_tag="PLE39_17770"
     CDS             complement(1386..1892)
                     /locus_tag="PLE39_17770"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNE70237.1"
                     /translation="MTTPASWVMAIIYWLHMLATVTWIGSLAAINLLVLPASQRTLKL
                     ADQLSFIAALQKKLEPLAWFSMGLLVATGLFQLSANSHYDGFFDTSTQWSLSILIKHG
                     LSLIMGIVSAVQTWEVIPAIHRTLMKKEKASPEELASLQRREVILLRTNLILSALILG
                     ATAFARAS"
     gene            complement(1896..2963)
                     /locus_tag="PLE39_17775"
     CDS             complement(1896..2963)
                     /locus_tag="PLE39_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70238.1"
                     /translation="MSEQTENTQPVTPQPENSQPETVETETAQAESAPQVDDTQPIKP
                     PKKPSRWRSILTGLFGLLLLVGLGAYGGYSSGIGIRQNAEDSVISKQLTEQYAFALTD
                     IQLGNYETAMQRLKFIIANDPGFPGAQQKLTEVMVMSVIPTPTLVPTPTSTPDFSGVE
                     SAYQRAQQLVNAQDWPAALEALDIVRKLDPTYKAAQVDGMYYFTLRNYGIALINQQGN
                     LEGGIYHLTLAERFGPLDNTANQLREGARMYITGASFWELDWKQAYNYFSQVGAGWPG
                     MWDGTMTGSQRLFISAMRYGDELFDQQSYCAAYEQYQIAAGLGTLDNRSQNHFQEAGI
                     QCYPPTEVPPTLEPTPITPAP"
     gene            complement(3248..4753)
                     /gene="murJ"
                     /locus_tag="PLE39_17780"
     CDS             complement(3248..4753)
                     /gene="murJ"
                     /locus_tag="PLE39_17780"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNE70239.1"
                     /translation="MNKLSRLTRNSLIIAFFFLVDKALAFVRTGIISRQYSDSVHMLD
                     TFNAANNLPDVLFALISGGALAMAFIPLLTEYLTTKDRAAAWDLFSRVANVAFLVTGS
                     FAIVIAIFAQPIVDANLGIAPGFGEEQRKLLAELMRLNLIGTIIFSISGLVMASLQAN
                     QHFIFPAMAPSMYNIGQIIGAIFLVPRYGIQGLVYGVIIGALMHLLIQVPMLFKFGFR
                     WTPSLDIRHTGLIEALKLMAPRLLTMGGIQLIVLARDNLASRLDQVGAVTSLTYGWMI
                     MQVPETILGTAIATAMLPALSELAARHDWVEFRSTIERALRVLIALTIPIAAVMAAGI
                     HPLVRGVFGFDEATSTLVTWTTRAYLMTLTGFTIHEIAARSFYARKEPLFPLYAVGLR
                     LVMFLGIGFIGLTFFKEIGAPIIAFAEIALLVEAIILFKWLSNRTHEPIHVGGSVVRG
                     LIAAVIGGVVTYAVALYLPGGAIITALIGMVVGGLIALAIVWSDAKILLKL"
     gene            complement(4756..4947)
                     /locus_tag="PLE39_17785"
     CDS             complement(4756..4947)
                     /locus_tag="PLE39_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70240.1"
                     /translation="MTEQNEKNCLNCNRTDEQVPLLHLTFKNETKYICPQCLPVLIHK
                     IHLIADKLPGVEIAPDAAH"
     gene            complement(5015..5803)
                     /locus_tag="PLE39_17790"
     CDS             complement(5015..5803)
                     /locus_tag="PLE39_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE70241.1"
                     /translation="MDLQLKNKIALVAASSRGLGYAAARTLAQEGCRVAINGRDEAKI
                     KGVAEKLSKETGAEVIGLGGDVSLPHVPEMLVAQTVEAFGGLDILITNTGGPTAGSID
                     ALDEAAWQKGIDLCLMAHVRLIKSALPHLRKSKSASVLTVTSYSVRQPIPNLLISNSV
                     RAATVGLTKSLALELGRENIRVNSILPGWTETERVTELMTDRAKKNSSTVGEETERQA
                     AESPFGRMGQPEEFANAAVFLVSPAASFITGVMLLVDGGTVKGV"
     gene            complement(5858..6139)
                     /locus_tag="PLE39_17795"
     CDS             complement(5858..6139)
                     /locus_tag="PLE39_17795"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HNE70242.1"
                     /translation="MLAILTIHLRLPLCTSLKEKRGRIKPLLSRLHREFNVSVAEMDL
                     QDTWDEAIIACAMLNSDAVVLRQSLQSVAKWVEANWTDGDVWDSKIENL"
BASE COUNT         1502 a         1541 c         1811 g         1508 t
ORIGIN      
        1 ctgattcaat ggcacagtta ttgatccgtg atggatatcg cgcaatggat gagcctgaca
       61 aggcggcggt attgatcgtg aacacgtgcg gcttcatcgg cccggcgaag gaagaatcct
      121 atcgtgtgtt gggtgagctt gccaaatcca agcacagcgg acaggttttg atcgccgctg
      181 gttgtttaac ccagcgctac ggcgtggagg tggctcagaa ggtgcagggg atcgatggca
      241 tcctcggtac gcgccgttgg atggatattg tgcaggttgt aaaagaagca cgcaaaggaa
      301 aacaccccga accgatctat catttgcccg aagcggcgac ggtcggtgtc gatgagcagg
      361 atgccttgcg ggtgagtgtg gcgggagcca gcgcctacct caaagtggca gatggctgtc
      421 gccgtccgtg tgcattctgt gccatcccgt tgatcaaagg tacggcagtt tcaaggcctg
      481 tggatgccat tgttaacgag gcgcggacct tgcgcgataa tggcgttcgc gaattggtgc
      541 tgatcgcaca agacaccacc gattatggtc atgaccttgg catgaaagat ggtcttgccg
      601 ttttgctgga acaattgacc gactctgtac cggatatgga ctggatccgc gtgatgtatg
      661 cctaccccgg ctatgtgacc gaccgcctca tcgaagtaat ggcaacccgc aaacagattt
      721 tgccttatct cgatatgccg ctgcaacatg cacatcccaa aaccttgtat cgcatgaaac
      781 gcccttcgaa tatcgaatgg gttcataaaa cacttgggaa aatgcgggcg gcaattcccg
      841 aattatccat ccgcaccact ttcattgtcg gctatcccgg cgaaaccgat gaggaatatc
      901 aggcgttgta cgatttcgta aaagagattc gctttgaccg tgtgggagcc ttccagtttt
      961 cattcgagcc ggggacgaca tccgcgcctc tgggcgaccc ggtgcctgct tctgttaaac
     1021 aggaaagata cgagcacctg atggagcttc agcaaaccat ctcacttcag gtgaatcaat
     1081 cctatgtcgg caagacactc gatgtgcttg tggaagggtt cgacaacggc atttccattg
     1141 gacggtctta tcgcgatgcg cccgaaatcg acggaatggt tttcattgaa gggaaggcaa
     1201 atgtgggtga gatcgtaccg gtccgcatca gcggcgcaat ggcctacgac ctgacgggtg
     1261 tgctggcgca atcacaacag caggtgatca aactataacc tgttgtgtgg agcagttcat
     1321 gcgcaagata aatcttgcgg tacaaaaagc ggctgatact tttcagtatc agccgctttt
     1381 gatttttagg atgctcttgc aaatgcagtt gcgccaagga tcagcgccga gaggatcagg
     1441 ttcgttctca gcaggatgac ctcgcgcctt tgcaggctgg caagctcttc cggactggct
     1501 ttttcctttt tcatcagtgt gcgatgaatg gccgggataa cctcccaggt ttggacggcg
     1561 ctcacaatgc ccatgatgag gctcaagcca tgtttaatga gaatggacag cgaccattgt
     1621 gtgctggtat caaagaagcc atcgtagtgc gaattggcgc tcaattgaaa cagcccggtt
     1681 gcaaccagta agcccatgct gaaccatgcc aatggctcaa gctttttttg aagggcggcg
     1741 ataaagctga gttggtcggc cagtttcaag gtccgctgtg atgcgggcag gacgagcagg
     1801 ttgatggcgg cgaggcttcc gatccacgtg actgtggcga gcatgtgaag ccagtagatg
     1861 atcgccatta cccatgaggc gggtgtggtc atggcttagg gtgcgggtgt gatcggtgtg
     1921 ggttccagtg tgggcggaac ttccgtcggc gggtagcact gtatccctgc ctcttggaag
     1981 tggttttggg aacggttatc cagggtgcca aggcctgctg cgatctggta ttgctcgtag
     2041 gcggcgcagt aggattgttg gtcgaagagt tcgtcgccgt agcgcatcgc tgagatgaag
     2101 agacgctgtg agccggtcat ggttccatcc cacatgccgg gccaacctgc tcccacctgg
     2161 ctgaagtagt tgtaggcttg tttccaatcc aattcccaga aacttgcgcc ggtgatgtac
     2221 atgcgggcgc cttctcgcaa ttggttggcg gtgttatcca gtggaccaaa acgctcggcc
     2281 agagtcaggt gatatatgcc gccttcgaga ttgccttgtt gattaatgag ggcaatgcca
     2341 tagttgcgca gggtgaaata gtacatgcca tccacctggg cggctttgta ggtggggtcg
     2401 agtttgcgga caatatcgag ggcttcgagg gcggcaggcc aatcttgcgc attgaccagt
     2461 tgctgggcac gttgataggc gctttcgacg ccgctgaaat caggggtgga ggtgggcgtg
     2521 ggcaccaaag tgggggtggg gatgacgctc atcaccatca cttcggtgag cttttgctgg
     2581 gcgccgggga agccggggtc gttggcgatg atgaatttca ggcgctgcat cgcggtttcg
     2641 taattgccca gctggatgtc ggtcaatgcg aaggcgtatt gctcagtgag ctgcttcgag
     2701 atgacggaat cttccgcatt ttggcggatg ccaataccgg aggagtatcc gccgtatgca
     2761 cccagcccaa ccagcaggag cagaccgaag agaccggtca ggatggagcg ccagcgggaa
     2821 ggtttcttgg gcggtttgat gggctgggta tcatccacct gaggggctga ttcagcctgg
     2881 gcggtttcag tttccacagt ttcgggctgt gaattttcag gttgaggtgt gacaggttgt
     2941 gtgttttcag tttgttcgga catgagtgta attatacttc cctttgattt tacggttttc
     3001 ccgtttttta acgaaaaaaa acagaaacct cgccgtgtcg aaataaattt tataaaaaga
     3061 tgatcctggc aacgtccccg ctgccgagga tggcggcggg agcagccgca gatttaggat
     3121 aacaatccgc gaaaatcagc gaaatttgcg gcaaaagccc tattgagctg tggctattcc
     3181 gccagagacg agatagccac aagttcttaa ttcaggttca ggcggggtgc gacctgctcc
     3241 ggtagattta caacttcagc agtatctttg catctgacca gacgatcgcc agcgcgatca
     3301 gtccgccaac caccattccg atcagggcgg tgatgatggc gccgccgggc aggtaaagtg
     3361 ccacggcata ggtcaccacc ccgccgatga ccgccgcaat caaccctctg acaaccgacc
     3421 cgccgacatg aatcggttca tgggtacgat tcgacagcca tttgaacagg atgatggctt
     3481 ccaccaaaag tgcgatttca gcaaaagcga tgatcggggc gccgatttcc ttgaaaaagg
     3541 tcaggccaat gaacccgatt ccaaggaaca tgacaaggcg caagcccact gcataaagcg
     3601 gaaacaaagg ctccttgcgg gcataaaacg agcgcgccgc gatttcgtgg atggtgaatc
     3661 cggtcagggt catcagatag gcgcgggtgg tccatgtgac gagggtggag gtggcttcgt
     3721 caaagccaaa gacaccgcgt acgagcgggt gaatgcctgc tgccatcacc gctgcaatgg
     3781 ggatggtaag tgcgatcaac acccgcaggg cgcgttcaat ggtggagcgg aattccaccc
     3841 agtcgtggcg tgccgcaagc tctgagagtg cgggcagcat ggctgtagca atggctgtcc
     3901 ccaggatggt ttcagggact tgcatgatca tccagccata ggtgagcgat gtcacagcgc
     3961 cgacttgatc aagacgcgag gccagattat cgcgggcaag aacgatgagt tgaatcccgc
     4021 ccatggtcag cagacggggg gccatcaatt tcaaggcttc gatcaaacct gtgtggcgaa
     4081 tatccaatga gggggtccag cggaatccga atttgaacag cattggcact tgaatcaaca
     4141 agtgcatgag cgcgccgatg atgacgccat ataccagacc ttgaatgccg taacgcggca
     4201 caaggaagat cgcaccaatg atctgcccaa tgttgtacat gctgggggcc atcgcaggaa
     4261 agataaaatg ctggttggct tgcagggaag ccatcaccaa tccgctgatg gaaaagatga
     4321 tcgtgccgat caggttcaag cgcatgagtt cagccaagag cttgcgctgt tcctcgccga
     4381 agcccggggc aataccgagg ttggcatcca cgatgggttg ggcgaagatg gcgatgacga
     4441 tggcaaagga acccgtcacc aaaaaagcca cattggccac gcgtgaaaac aggtcccatg
     4501 cggcggcgcg gtctttggtg gtgagatact ccgtcagcag cggaatgaac gccattgcca
     4561 gcgctccgcc tgagatgaga gcgaaaagta catcgggcaa attgtttgcg gcgttgaagg
     4621 tatccagcat gtgaaccgaa tcggaatact gccgcgagat aatacccgta cgcacgaagg
     4681 cgagggcttt atccaccaga aagaaaaatg caatgatgag cgagttacgg gtaaggcgtg
     4741 aaagtttatt catttttaat gagccgcgtc tggcgcgatc tccacgccgg ggagtttatc
     4801 tgcgatcaga tggatcttat ggatcaacac cggcaggcat tgcgggcaaa tgtatttggt
     4861 ttcattttta aaagttaaat gcagcaacgg cacttgttca tcggtgcggt tgcaattcag
     4921 gcagtttttt tcgttttgtt cggtcatggt tctcctttgt tttaacgttt taacgttcga
     4981 acgtttgaac gttaaaacgt ttgagtgtaa aaaattaaac ccctttaacc gtgccgccat
     5041 ccacaagcag catgacaccg gtgatgaatg atgcggcagg ggagacgagg aacacagcgg
     5101 cattggcgaa ctcttcaggt tgacccatcc tcccaaacgg actctccgcc gcctgccgtt
     5161 ccgtttcttc tccgaccgtg gaactgttct tttttgcccg gtcagtcatc aactctgtga
     5221 ctcgttccgt ctccgtccaa cctggcaaaa tcgagttgac gcggatattc tccctgccaa
     5281 gctccagcgc gagtgatttg gtcaacccca ccgttgccgc gcgaacactg ttcgagatga
     5341 gcaggttggg aattggctgc ctgaccgaat acgaagtgac cgtcagcaca ctggcggatt
     5401 ttgattttcg caaatgaggc agcgccgatt tgatcaagcg gacgtgcgcc attaaacaca
     5461 ggtcaatgcc tttttgccat gcggcttcgt cgagggcatc aatggaacct gcggtggggc
     5521 cgcccgtgtt ggtgatgaga atatccaatc cgccaaaggc ttcaacagtt tgggcgacca
     5581 acatttcagg tacgtgaggc aggctgacat caccgcccaa cccgatcact tccgccccag
     5641 tttccttgct aagtttctcc gccactcctt tgatcttcgc ctcgtcccgc ccattgatgg
     5701 cgaccctgca gccctcctgt gccaaagttc gcgcggcagc atatcccaac ccacgcgagg
     5761 atgcggcgac gagggcgatt ttatttttga gttgtagatc catgttgtct cctgtggtgt
     5821 aacgttgtaa cgttcaaacg tttgaacgtt acaacgttta taagttctct attttgctgt
     5881 cccaaacatc tccgtcggtc cagtttgctt ccacccattt tgccacactt tgcagagact
     5941 gtcgcaagac caccgcgtcg gaattgagca tcgcgcaggc gatgatggct tcatcccatg
     6001 tatcctgcaa atccatctca gcgacagaca cattgaattc acggtgcaaa cgagagagca
     6061 gcggcttgat tcgtccgcgc ttttctttga gggaggtaca taagggcagt cgaaggtgga
     6121 tggtgaggat agcgagcatt tggtgattgt aaagaggttt gtatctacga gtataataag
     6181 aagatttttt caagatacag ctatttatgg agtatattaa tttataaatt acattttttg
     6241 atgtgagatt tcgtatttta gtttagtttg tctaaaaaat actgcaaaaa acattttaaa
     6301 gcaaaaaaaa aattaaaaaa atatttatct aagaatttaa aaaaagagat tttttgataa
     6361 ta
//
LOCUS       DAOQJC010000177         6324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_6934_length_6324_cov_6.142766, whole genome shotgun sequence.
ACCESSION   DAOQJC010000177 DAOQJC010000000
VERSION     DAOQJC010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6934_length_6324_cov_6.142766"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..2601)
                     /locus_tag="PLE39_17800"
     CDS             complement(58..2601)
                     /locus_tag="PLE39_17800"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE70243.1"
                     /translation="MAAQQKQTSSPQNQFFTNTFGDALKYLRKRAHLTQDELGRAVGY
                     SREQIARLENGSRVPDLAVVAALFVPALDIQHQPELIQRLLELAGQIRSAPGESQHIT
                     VAHTTQTKTQRIVEVVSQSAPLPYTLPSPLFPLIGRVSEVESLCALLLGQARLITLIG
                     TAGIGKTRLALETAHALTGNFSHGACFVELAPIQNAEAVPGAIASALGITPTADQPIE
                     TAVAAHLAPHETLLVLDNCEHVLDAAVYFDKLLAVAPKLKLLCTSRTALDLYGEHEWE
                     VSPLSLPILSQLPDPDQLSQIAAVQLFLARIRTFDSAFRLDSSNAVSIATLCVALDGL
                     PLALELAAARVREIKPADLLHQIVSTRSRPQLSSTLLQQTKRNISARHRTLHEAIAWS
                     YRLLSPAQQAAFIRLGVIVGGCTVEAAQNICDTDLPTLQALASASLIQLESNPSPGRV
                     NMLETLRSFALEQLIHANLLNTLKRKHAAHFAEYAGVIFKEIRGGQQSQWLAQTRLEH
                     DNFRSALLFAVEEQDSHLAVSISGGLWWFWYRQGFLSEGREWLDKSLRIPMKHQPSET
                     QKRARATALNGAGSMACEQCDYPSAMAYHKEGLALRYELEDGEGIATVLHNMALVARS
                     QGDYPRAIELFEKSLTMEKETGSDSSLAMSYANIGITAGEMNDLPLAKSWLNRALEKV
                     NVDELPWETAFIALNLAQILYWQEDFTQAEALARQSLRSFEEQGDQLYGPEAQLILAQ
                     TAIEHGDFSLAQSLCGNVLAYYQGLNDEHGISNVLSVLAWITLHEDHNNEGAATAARL
                     FSESMFLREKNKRALSPMEKIRNQMLEDAIHRLASQQPGSREAADKKEP"
     gene            2780..2962
                     /locus_tag="PLE39_17805"
     CDS             2780..2962
                     /locus_tag="PLE39_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70244.1"
                     /translation="MDRRLLVIRMTEVVEVTETITIHVKNDALKDSETDVVVEETELR
                     WVQLEFTDLLIKAPEK"
     gene            2982..5135
                     /locus_tag="PLE39_17810"
     CDS             2982..5135
                     /locus_tag="PLE39_17810"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNE70245.1"
                     /translation="MKKRIALLVLILNLVAAALQVQPARAANAVVGTGTPASCTETAF
                     DNALTTAGSGGGTITFNCGSGTHTITFSTSKTVNLGNVTIDGGNSIILAGSSTVRQFF
                     VGGGVTFTLKNITLRDGDSLVGGGAIEASTATVILDSVQILNNYSAVQGGAIYCYDGT
                     VSASDTLFSGNESSTGGAIFNDGCNITVNGSTFQNNKTSTSGGAIYNSMLGVLQVSSS
                     QFTGNQSLDGGGIFNASGATATLNAVTMSSNSGGHGGGVENSGTMTINDSIIDSNSVT
                     GSGGGIWNMGGALTLARTSVNNNRAFEGAGINSYGTQVQITNANITNNVTTGTHGGGI
                     FINAGTASITNATISGNQAVGAAADGGGIYHNSNDNLTLTNVTLVSNQAGARGGGLYH
                     YGNYAILTNVTIGNNTAVMAGPAIYEDSPNTAVVQMKNSVVFGSAGNCDGGVFQSLGY
                     NISKGSCPSLNQATDQDNFAGDIAMGALAFNGGIFPMQTMMPLSGSPVIDAGDACSAT
                     DQLGAARPVGAGCDIGAVEFGASTSTFADVPSSYWAWQFIERLYAAGITGGCSTSPLN
                     YCPESGVTRAQMAIFLLKGIHGSTYTPPAVGASTGFNDVSTSYWAAAWIKQLAAEGIT
                     SGCGGGNYCPDATVTRAQMAIFLLRAEHGSAYMPPAASGIFTDVPVGYWADAWIEQLA
                     AEGITSGCGTGIYCPEADVTRAQMAVFLVRTFKLP"
     gene            5219..>6324
                     /locus_tag="PLE39_17815"
     CDS             5219..>6324
                     /locus_tag="PLE39_17815"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNE70246.1"
                     /translation="MKIQILVSLLVILACIMIPSQVPPAAALGNVIYVSTAGSNSTGD
                     GSISNPFQTIGHAITTATAGDEIVLRGSPAVADNHYEESIRIEIPNITVRSQDGEWAV
                     IECPFNNENITECVRLDVDSSGSKIRSLEIIGGYYYGIKLETRWDWGDPSDRSGASNI
                     LIEGVVIHNTGRDAIKITPGCDDVTIRRVEIYNTGVRDNSNAEGIDNVNGDRMIVQDS
                     YLHDIATTGIYFKGGAMDGIVERNRIERTGEAGILIGFDTSPEYFDLTVNPSYYENIR
                     GIVRNNIISDIPYSGIGLYAAQNAQVLNNTIINTAQVGHSPIYFGITYQDWDPAATRP
                     SSVNPIIQNNLISQPAGQSGECVFIRYSEDLGGLS"
BASE COUNT         1508 a         1544 c         1718 g         1554 t
ORIGIN      
        1 acgccgtaaa ataagatacg cgttacatcc cataaaaaga gccgtgagat caaaatctca
       61 cggctctttt ttgtctgcag cttccctgct tccaggttgc tgacttgcca atcgatgaat
      121 tgcatcctca agcatttgat ttctgatttt ttccataggt gacaaggcac gcttattttt
      181 ttcgcgtaaa aacatagact cggaaaacag tctggcggct gttgccgcac cctcgttgtt
      241 atgatcttcg tgaagggtaa tccatgccag aacggaaagt acatttgaga ttccatgctc
      301 atcattgagc ccctgataat atgccagaac attgccgcac agagattgag ccagcgaaaa
      361 atcgccgtgt tcaatggcgg tctgtgccaa aatcagttgt gcttcaggcc cataaagttg
      421 atcaccctgc tcttcgaacg accgcagact ctgacgggcc aatgcctccg cctgtgtaaa
      481 atcttcctgc cagtacaaaa tttgggcgag atttagtgca ataaaagccg tttcccaagg
      541 gagttcatca acattcactt tttcaagcgc gcggtttaac cagctttttg ccagtggaag
      601 atcgttcatt tcgccagcgg tgataccgat gttggcataa ctcattgcca gagaactgtc
      661 actgccagtc tccttttcca tggtaagaga tttttcaaat agttcaatgg cgcgtggata
      721 atctccctgg ctgcgggcga ccagagccat attgtgcaat acagtggcga ttccctctcc
      781 atcttccaat tcataccgta atgccaaccc ttctttgtga tacgccatgg cagaaggata
      841 atcgcattgt tcacatgcca tggaccctgc tccattgagc gctgtagcac gtgcccgttt
      901 ttgggtttcg ctgggttgat gtttcattgg aattcgtaaa cttttatcga gccactccct
      961 gccttcactc aagaatccct gtcgatacca gaaccaccat aacccgccag aaatggaaac
     1021 cgccagatgc gagtcctgct cttccacagc aaagagcagc gcagacctga agttatcgtg
     1081 ttcgagtctc gtttgggcca gccactgaga ttgctgtcca ccacggatct ctttgaatat
     1141 gaccccggca tattctgcaa aatgggcggc gtgttttctt ttcaaagtat tcaacagatt
     1201 ggcgtggatc agttgttcca atgcaaagga tcgcaaagtt tcaagcatat tcacccgccc
     1261 cggggacgga ttgctttcaa gttgtatcag gctggccgat gccaaagcct gcaaagttgg
     1321 tagatctgtg tcgcatatat tttgggcggc ctccactgtg cagccgccaa caatgactcc
     1381 cagccgtata aaggctgcct gttgagcagg ggaaagcagc cggtaactcc atgcaatggc
     1441 ttcatgcagg gttcggtgcc tcgctgaaat attgcgcttg gtctgttgca gcaaggtgga
     1501 ggatagttgt ggacggctgc gggtgctgac gatctggtgc aacaggtcag caggtttgat
     1561 ttcacgcacc cgggccgcgg ctaattccag tgcaagaggc aatccatcca gtgcaacgca
     1621 taaagtggcg atggaaacgg cgttgcttga atccaacctg aaagcggaat cgaaggtccg
     1681 gattcgcgcc aaaaataact ggactgctgc gatctgactc agctggtcag ggtcaggcaa
     1741 ctgtgacagg atcggcaggg atagtgggga gacttcccat tcgtgttcgc cgtacaggtc
     1801 aagtgcggtg cggctggtgc agaggagttt gagtttgggg gcaacggcga gcaatttatc
     1861 aaaatacaca gccgcatcaa ggacatgttc gcagttatcc aatacaagca gggtttcatg
     1921 aggcgcaagg tgggcggcaa ctgcggtttc aatgggttgg tctgctgtgg gggtgatgcc
     1981 caacgccgag gcaattgcac cggggacggc ttctgcgttt tggatgggag ccagttctac
     2041 aaaacatgcg ccatgtgaaa aattaccagt cagtgcatgg gctgtttcaa gggccagcct
     2101 tgtttttccg atacctgctg tgccgatcag ggtgatcagc cgcgcttggc cgagtaaaag
     2161 cgcgcaaaga gattcgactt ccgaaacgcg gccaatcagc ggaaagagcg gagagggtag
     2221 tgtgtagggt aatggcgcag attggctaac gacttcaacg attctttggg ttttggtttg
     2281 ggtggtatgg gcaacggtga tgtgttgaga ctcgccaggg gcgctgcgga tctgcccggc
     2341 taattcgagg aggcgttgaa tgagttcagg ttgatgctga atatccaatg ctgggacgaa
     2401 gagggcggca accacggcaa ggtctggcac acggcttcca ttttcaaggc gggcgatctg
     2461 ttcgcggcta tagccgacag cgcgccccaa ttcatcttgt gtcaggtggg cgcgttttcg
     2521 taaatatttg agcgcatcgc caaatgtgtt tgtaaaaaac tgattctgtg gggaagaggt
     2581 ttgcttttgt tgggcggcca tgggtgaatc atagcatatt caatggagtg ggggcatttt
     2641 ttaggaggga tgggtcaacg gcaattgagg ccgttcggat ggtatcaagt ctcggttatg
     2701 ggggagtcgt cttttgtcca tatttttgcc tgtcacaaaa tgtcaccttt ggtcacgacc
     2761 caacctgtat gatgcggaca tggatcgccg tttgcttgtc atcagaatga ccgaagtcgt
     2821 agaggtgacc gagacgatca cgattcacgt caaaaacgat gccttgaaag attcagagac
     2881 ggatgtagtt gtggaagaga cggaacttcg atgggttcag cttgaattta ccgatctgct
     2941 gataaaggcg ccagaaaaat aatatcaaca aggagatgac catgaaaaaa agaattgctt
     3001 tgctggtttt gattttgaat ttggtggctg cggctttgca ggtgcagcct gcccgcgctg
     3061 ccaatgcggt ggttgggacg ggaacacccg cttcctgtac agagaccgca ttcgataacg
     3121 cattgacgac ggctgggagt ggcggtggta cgatcacgtt caattgcggt tcaggtacac
     3181 acacgatcac ttttagtact tccaagacgg tcaatttggg aaatgtaacc attgacggcg
     3241 ggaattcgat catccttgcg ggttcgtcaa ccgtgcgtca gttttttgtc ggcggcggag
     3301 taacgtttac cctgaaaaac atcactttgc gcgatggtga ttcgttggtg ggtggcggcg
     3361 caattgaggc atccacggca acagtgattt tggattcggt gcagatcctg aataattatt
     3421 cagcggttca gggcggtgcg atttattgtt acgacggcac cgtgtcggcc agcgacaccc
     3481 tgttcagtgg gaatgaatcg agtacgggag gcgcgatctt caatgatggt tgcaacatca
     3541 cggtcaatgg ttcgaccttc caaaacaata agacgtctac cagcggcggc gcgatctata
     3601 acagtatgct tggtgtgttg caggtcagca gcagtcaatt cactgggaat cagtctcttg
     3661 acggcggcgg tattttcaat gcaagcggcg ccacggctac ccttaatgca gtgacaatga
     3721 gttccaattc aggcgggcat ggcggtggcg tggaaaacag cggcaccatg accatcaatg
     3781 acagcatcat tgactcgaat tctgttactg ggtctggcgg cggcatctgg aatatgggcg
     3841 gcgcgcttac gctggcacgt acttcagtga ataacaacag agcctttgaa ggtgcaggga
     3901 tcaacagcta cggcacacag gtacaaatca ccaatgccaa tatcacaaac aatgtcacca
     3961 cgggtacgca tggcgggggt attttcatca atgctggcac ggcttccatc accaatgcca
     4021 ccatcagcgg aaatcaggct gttggagcgg ctgccgatgg aggcggtatc tatcacaatt
     4081 cgaacgataa tctgacgttg accaatgtga cgctggtttc caatcaggcg ggcgcacgcg
     4141 ggggcgggtt gtatcactat ggtaattatg ccattttgac caacgtgacg attggtaata
     4201 atactgctgt catggctggg cctgcgatct acgaagattc gccgaacacc gctgtggtcc
     4261 aaatgaagaa cagtgtggtc tttggcagtg ctggcaattg tgacgggggg gtgttccaat
     4321 cacttggcta caacatcagc aagggatcat gcccgtcttt gaatcaggcc accgatcagg
     4381 ataattttgc aggtgatatt gccatgggag cgcttgcctt caatggaggc atattcccca
     4441 tgcagaccat gatgccgctt tcaggcagcc cggtgattga tgcaggagat gcctgctctg
     4501 ccaccgatca gcttggcgca gcgcggcctg tgggtgcagg ttgtgatatt ggtgccgtgg
     4561 aatttggtgc atccacttcc actttcgccg atgtgcccag ttcctattgg gcctggcaat
     4621 tcatcgaacg tttatacgcg gctggcatca ccggtggttg ttccaccagc ccattaaatt
     4681 actgccccga atctggtgtc acccgtgcgc aaatggccat cttccttctt aagggaatcc
     4741 atggctcaac ctacactccg cctgctgtgg gtgcaagcac tggcttcaat gatgtttcca
     4801 caagttattg ggccgcagca tggatcaaac aactggctgc tgaaggcatc accagcggtt
     4861 gcggcggcgg aaattattgc cccgatgcca cggtcacccg cgcccaaatg gccatcttcc
     4921 ttctgagagc cgagcatgga tccgcctata tgccgcctgc cgccagtggg attttcacag
     4981 atgtccccgt cggctattgg gcagatgcct ggatcgaaca gttggccgcc gaaggcatca
     5041 ccagcggatg tggtacagga atttattgcc ccgaagcaga tgtgacccgc gcccaaatgg
     5101 cggtttttct agtgcggacc ttcaaactgc cgtaacgaat cgagcgcccc gcaaactgat
     5161 tcaacccaat ttgcggggcg ctccctttgg gcctgctttt gcgaaaggaa aaataaaaat
     5221 gaagatccag atattggtca gcctgcttgt cattctagcc tgcatcatga tcccatctca
     5281 agttccacct gccgcagcac tgggaaatgt gatctatgtc tcaacagcgg ggagcaacag
     5341 tacaggggat ggcagcatct ccaatccgtt tcaaaccatt ggccatgcca ttacaaccgc
     5401 caccgccgga gacgagatcg ttttgcgcgg ctctcctgca gttgccgaca atcactacga
     5461 agaaagcatc cgcatcgaaa ttccgaacat caccgtccgc tcgcaggatg gcgaatgggc
     5521 agtgatcgaa tgcccgttca acaatgaaaa tatcaccgaa tgtgtccgcc ttgatgtgga
     5581 tagttccggc agcaagattc gcagcctcga aatcatcggc ggatattatt atggtatcaa
     5641 acttgaaacc cgttgggatt ggggagatcc ctcagatcgc agtggagcct ccaatatttt
     5701 gatcgaaggt gtggtgatcc acaatacagg gcgcgatgcg atcaagatca ctcctggctg
     5761 tgacgatgta accatccgtc gtgttgaaat ttataacacc ggtgtgcgcg ataactcgaa
     5821 cgccgaaggg attgataacg tgaacggtga ccgtatgatc gttcaagaca gctacctgca
     5881 cgatattgcc acgaccggta tctatttcaa aggcggggct atggatggca ttgttgagcg
     5941 gaatcgcatt gagcgcacag gggaagcggg gattttgatt ggcttcgata ccagccccga
     6001 atatttcgac cttacggtaa atcccagtta ctatgagaac atccgcggca ttgtgcggaa
     6061 caatattatc agcgatatac cttactcggg tattggctta tatgccgcac aaaatgcgca
     6121 agtcttgaat aacaccatca tcaacactgc gcaggttggg catagcccca tctactttgg
     6181 tattacatac caagattggg accccgctgc cacacgacct tcatctgtga atccgatcat
     6241 ccaaaataat ctcatttcac agccagcagg gcagagcggc gaatgtgttt ttattcgcta
     6301 ctcagaagat ctgggcgggc tttc
//
LOCUS       DAOQJC010000178         6244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_7066_length_6244_cov_10.393925, whole genome shotgun sequence.
ACCESSION   DAOQJC010000178 DAOQJC010000000
VERSION     DAOQJC010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7066_length_6244_cov_10.393925"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1196
                     /locus_tag="PLE39_17820"
     CDS             114..1196
                     /locus_tag="PLE39_17820"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE70247.1"
                     /translation="MKNFPHRLGLQQRVLPSYRVPFFDLLAQSCESMSLFAGQARPVE
                     MIASGRPQVAAYHEAKNIHLFSGAFYLCHQQNFISWLEEWHPHTLIVEANPRYLATSA
                     AVNWMHARGRKVIGWGLGSPPLSGPLAGFRQKSRISFLSKFDALLAYSQRGADEYAAL
                     GFPREKIFVAHNSVSPKPTFNLQPATFKPTPSILFVGRLQARKRVDHLLRACAEMDSK
                     PRLMIVGDGPERAALESLAKDVYPSAEFIGAKHGADLKPYFEQADLFVLPGTGGLAVQ
                     EAMSYGLPVIVAKGDGTQDDLVRDGNGWQIQPEDYGALVSTMKNALSDTARLRKMGTE
                     SFRIVSEEINIQKMVEVFVDALNSVA"
     gene            complement(1229..2797)
                     /locus_tag="PLE39_17825"
     CDS             complement(1229..2797)
                     /locus_tag="PLE39_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70248.1"
                     /translation="MKTETERLNILILVFGLLLGAYARFLPASMVGFPVTDGGLFYEM
                     VKAIQAGGYNLPSFVEYNGISIPFAYPPLGFYTAALISDIFNFPLLEVFRWMPAVGSV
                     LFSIAFYPMAAAILKSPLKGSLATVFFALLPRSISWYIMGGGITRVLGHIFLMLILFS
                     AYKLFTAPSRKYLVLTILFGSGVILSHPEASLHTVFLCAVLWLFYGRSKEGIKNASLV
                     ALGVIALTAPFFLTVISRHGLQPYLSAAQTGFQNPLAWVAILTGSFADEKFVTVLSAL
                     ALLGFVVAIARREFLLVVWLFLPAVVDPRSAASISIIPWAMLAAIGFVDIVIPGLYAL
                     KHNLQEAGEIESNWSTYFIKSSAIKFALTALIFYAFFGTVLSDQVYPKVSLTSSDRAA
                     MEWVAGNVPPQSRFLILTGETEAFADAVAEWFPVLSGSYGIATIQGYEWMPDKIFLTK
                     LDEYYALQACMDKTSTCVDEWALKEDHPFEYVLISRDGLSENKNSLLISLEQSGGYQL
                     IHEETDVYIFEKNP"
     gene            complement(2886..3587)
                     /locus_tag="PLE39_17830"
     CDS             complement(2886..3587)
                     /locus_tag="PLE39_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70249.1"
                     /translation="MKAFHSFVLSIPYLFLKKIPYAWVFVIALWAWPPVVSGIFVGII
                     LLGLWFMVMQQKAWEAEIAREYRVLYRDQPHAPVMYQVRNFVLVCAAGALIGWVADGK
                     LGLSSLQWTLMLIGVMLLYKDAMLFGAGTVYLVTNKGIAVRYIPGHVDYRLFFGYDEI
                     RNVERIGPVKSLPMAWSVLTPQRKILENGFLLKPKKLNGFTKTIEEVVLCPADPEGFL
                     KHMPPSISVKEAVTR"
     gene            complement(3596..4756)
                     /locus_tag="PLE39_17835"
     CDS             complement(3596..4756)
                     /locus_tag="PLE39_17835"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE70250.1"
                     /translation="MTFQLVYYSQQDPKWKEDILGFGDSRDTIGYVGCALTSVAMLIS
                     GHGFIETPKTLNQKLKNVNGFVSAGIRWDMVNQIYPQINVKSNISCGNTDAPLGLIDA
                     SIAAGQPVIVMVDSTPAPGLLTHWVVLYGREGNDYLMLDPWPYQTDVTKKTYLMPRYS
                     QGNSLERAIMHVIAYECFTASGGITQPIETDQTTPTTGTTTTSTTLARVKADLTWGLN
                     IRSSIDTSSSSNIVAVVSAGTLLTLLGADDISKIGAVNQWVRVNDGKGHEGYCAAWYL
                     EKVQVAAPVVETESGSTSTEEAGGSTSTPPKPAPMIVTVKARVGRLGAKVYKTASAKS
                     EIVSTEKMRAKLTVVEDAAKARAKLGVAGKWVNVKNKEGKKGYVSAELVSEV"
     gene            complement(4854..5345)
                     /locus_tag="PLE39_17840"
     CDS             complement(4854..5345)
                     /locus_tag="PLE39_17840"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNE70251.1"
                     /translation="MKKPNWILIGILFGVSLFIGFTIFSAAVGAIFPKLHYVSKPLLC
                     SGDFKIETTRYSYKPGQVGWQHNIYCDGREITLLAVFTTGLLFSLGIFMAACIRYWKA
                     LFNPADFGSLAKDLKQADKPARGKKKKSPLERMAELKEMRDQNLISDVEYERKKTEIM
                     NEL"
     gene            complement(5369..6112)
                     /gene="budA"
                     /locus_tag="PLE39_17845"
     CDS             complement(5369..6112)
                     /gene="budA"
                     /locus_tag="PLE39_17845"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HNE70252.1"
                     /translation="MERTTLRRIRKRKADNKVYLCAPVNSLVEGIYEQNIPFYEIKKH
                     GDFGLGTFNDLDGEMMMVDGEIFRVGSDGRVNQITDDDVCTPFACVTNFQPFSEETLE
                     REVSYVDFLKWMVELMPSPNIFYAFRITGEFGYVKTRSVPKQENYRPLVEVAKEQPTF
                     EFNDVHGTMVGFYTPSFMASLSVPGVHLHFLTDDKSSGGHLLECRPRNVKLELQYLYT
                     LELSLPSNLDYLTWDFQRDIGKDLDKAEK"
BASE COUNT         1615 a         1741 c         1488 g         1400 t
ORIGIN      
        1 tttcttgcca aaacacaacc cgccattgtg gacgccgtgc aaaattcaag cgagtggtgc
       61 gagcaatacc acgatgatgt ggctttcatc ttcacacgct gcgagccgct gccatgaaaa
      121 attttccaca tcgccttggg ttgcagcaac gcgtgcttcc cagttaccgt gtcccatttt
      181 ttgatctgct cgcgcaatcg tgtgaaagca tgagtctgtt tgcaggccag gcccgccccg
      241 tggaaatgat cgcgagcggc aggcctcagg tcgctgcata tcacgaagca aaaaacattc
      301 atcttttcag cggcgcgttc tatttatgtc atcaacaaaa tttcatttca tggctcgaag
      361 aatggcatcc acacaccttg attgtggaag cgaacccccg ctatctggcc acatctgccg
      421 ctgtcaattg gatgcatgcc cgtggaagaa aggtcatcgg ctggggattg ggttcgccgc
      481 ctctttctgg acctctggca gggttccgtc aaaaaagcag aatctcgttc ctaagcaaat
      541 tcgacgcgct cctcgcgtac agtcaacgcg gcgcagacga atatgcggcc ctcggcttcc
      601 cgcgcgaaaa aatcttcgta gcacacaact cagtttcgcc aaaaccaacc ttcaacctgc
      661 aacctgcaac tttcaaaccc acgccctcaa ttctcttcgt cggccgcctt caagcccgca
      721 aacgcgttga ccatctcctc cgcgcctgtg cggagatgga ctcaaaacca cgattgatga
      781 tcgtcggtga cggccctgaa cgcgccgcat tggagtcact tgcaaaagat gtctaccctt
      841 cggcggagtt catcggcgca aaacatggcg ctgacctcaa accctatttc gaacaggctg
      901 atctttttgt gctgccgggc accggcgggc tggcagttca agaagcgatg agctacgggt
      961 tgcccgtcat cgttgccaaa ggggatggca cgcaagatga ccttgtccgt gatgggaatg
     1021 gctggcagat tcaacccgaa gattatggtg cgctggtttc cacaatgaaa aacgcgctgt
     1081 cagacacggc gcgtttgcga aagatgggaa cagagtcgtt ccgaatcgtt tccgaagaga
     1141 tcaacatcca aaagatggtg gaagtgtttg tggatgcgtt aaacagcgtg gcataaaaaa
     1201 tcgcggactg aagtccgcga tccgataatc aggggttctt ctcaaaaata taaacatccg
     1261 tctcttcatg aattaattga tacccacccg attgttcaag cgaaatcaaa agcgaatttt
     1321 tattctccga caaaccatcc cgcgaaatga ggacatactc aaagggatgg tcttctttta
     1381 aagcccattc atccacgcaa gtggaagttt tatccatgca ggcttgcagg gcatagtatt
     1441 catccagttt cgtcaaaaag attttgtcgg gcatccattc gtaaccttga atggtggcaa
     1501 tgccataact gccgctcaac acagggaacc actccgccac tgcatcggca aaggcctcgg
     1561 tttcgccagt gaggatcaaa aaacgacttt gcggcggaac attccccgcc acccattcca
     1621 tcgccgcacg atctgatgag gtgagactca ccttggggta cacctgatcc gaaagcacag
     1681 tcccgaaaaa cgcgtagaag atcaacgccg tcaacgcaaa tttgatcgca gacgatttta
     1741 taaaatacgt gctccaattc gactcgatct cacccgcctc ctgcaaattg tgtttcaacg
     1801 catacaaccc ggggatgaca atatccacaa agccgatcgc cgccagcatg gcccatggaa
     1861 tgatcgaaat cgaagccgca ctgcgcggat ccacgaccgc aggcaggaag agccacacca
     1921 ccagcaaaaa ctcacgcctt gcaatggcga cgacaaaccc cagcaaagcc agtgcggaga
     1981 gcaccgtcac aaatttttca tcggcaaatg aacctgtcag aatcgccacc cacgccagcg
     2041 gattttgaaa cccggtctgg gcggcactca gataaggctg gagtccgtgg cgggagatga
     2101 cagtgagaaa aaatggcgcg gtcagggcga tcacacccaa agcgacgagt gaagcgttct
     2161 taatcccctc tttggatcgg ccataaaaca gccacaacac cgcacacaga aaaaccgtgt
     2221 gcagcgacgc ttctggatgg ctcaatatca caccgcttcc aaatagaatc gtcagcacca
     2281 ggtattttct ggaaggcgct gtaaatagtt tgtacgcgct gaaaaggatc agcatcagaa
     2341 agatatgccc cagcacgcgt gtaatgccgc cccccatgat gtaccaggag atcgaacgcg
     2401 gcagcagcgc gaaaaaaaca gttgccagac tgcccttcaa tggagatttc aaaatcgccg
     2461 ccgccatcgg gtagaacgca atcgaaaaaa gcacactgcc caccgcgggc atccagcgaa
     2521 agacttcaag cagcggaaag ttgaaaatat ccgagatcag ggctgcggtg taaaacccca
     2581 aaggcgggta cgcaaacggg atcgatatac cgttgtattc cacaaatgac ggcaggttat
     2641 acccgcccgc ctgaattgcc ttcaccattt cgtaaaacag cccgccatcc gtcaccggaa
     2701 aaccgaccat gcttgcgggc aaaaaacggg cataggcacc caacaacagc ccgaagacca
     2761 aaatcaaaat attcaagcgt tcggtttctg tcttcaagat cacctcttgg tcaaagcgga
     2821 acgcggacaa aagtccgcgt ttttttcaat aacggactga agtccgcagc tcactgaata
     2881 aaatatcacc gcgttacggc ttccttcaca gaaatcgaag gcggcatgtg tttgaggaat
     2941 ccctcggggt cggcggggca caaaaccact tcttcgatgg tcttggtaaa tccattcaat
     3001 tttttaggct tgagcaaaaa tccgttttcg aggatcttgc gctgcggagt gagcacgctc
     3061 cacgccatcg gcagggattt caccgggccg atcctttcca cattgcggat ctcgtcatag
     3121 ccaaagaaca aacggtaatc aacatgcccg ggaatgtacc gaaccgcaat gcccttgttc
     3181 gtcaccagat acacggtgcc cgccccaaag agcatcgcat ccttgtacaa caacatcaca
     3241 ccgatcagca tcaaagtcca ctgcaaggat gaaagcccga gctttccatc tgccacccaa
     3301 ccgataagcg cccctgccgc gcagaccagc acaaaattcc gcacctgata catcacgggc
     3361 gcatgcggct ggtcacggta caaaacacga tactcccgcg cgatctccgc ctcccaggct
     3421 ttttgctgca tcaccatgaa ccacagcccg agcagaatga tccctacaaa aatccccgaa
     3481 accacaggcg gccacgccca caaggcaatg acaaataccc aggcataggg gatcttcttc
     3541 aaaaacaaat aaggaatgga caggacaaaa gaatggaatg ctttcatgtc agcacctaca
     3601 cttctgaaac caactcggcg ctcacgtatc ctttcttgcc ttccttattc ttcacgttca
     3661 cccatttacc cgccacgccc agtttggcac gcgcctttgc ggcatcttcc accacggtga
     3721 gtttggcgcg cattttttca gtggatacga tttccgattt cgctgatgcg gtcttgtaca
     3781 cctttgcacc cagcctgccc acacgggcct tgaccgtcac gatcatcggt gcaggcttcg
     3841 gcggtgtgga tgtagagccg cccgcctctt ctgtggacgt tgaacctgat tctgtttcaa
     3901 ccacaggagc cgccacttga accttttcca gataccatgc cgcgcaatac ccctcatgcc
     3961 ctttaccatc attcacccgc acccactgat tgaccgctcc gatcttcgaa atatcatccg
     4021 cgcccaacag ggtgaggagg gtacccgcag aaacaacagc aacgatattg ctcgaactgg
     4081 aggtatcaat acttgagcgg atgttcaaac cccaggtcaa atctgctttg actcgggcca
     4141 gggtggtgct ggttgtggtc gtacccgttg tcggcgtagt ttgatccgtt tctatcggtt
     4201 gggtgattcc gccgctggcg gtgaaacatt cgtaagcaat gacatgcatg atggcgcgtt
     4261 ccaatgaatt cccctgactg taacgcggca tgaggtaggt ctttttggtt acatcggtct
     4321 ggtagggcca cgggtcaagc atcagatagt cattcccttc acgaccgtaa agcacgaccc
     4381 aatgagtgag caggccgggc gcgggggtgc tatccaccat cacgatgacg ggctggcctg
     4441 ccgcgatgga ggcatcaata aggccgagcg gtgcatcggt gttgccgcac gagatgttgc
     4501 ttttcacatt gatctgtgga tagatctgat tgaccatatc ccagcgaatg cccgcgctga
     4561 caaagccgtt gacgttcttc aatttctggt tgagggtctt gggggtttcg ataaatccat
     4621 gcccgctgat gagcatggcc acactcgtca acgcgcagcc gacataaccg atcgtgtcac
     4681 gcgaatcgcc aaagccaaga atatcttcct tccatttcgg gtcctgctgg gagtagtaca
     4741 caagctggaa tgtcatgggg cacctctata aagttggaat atcacgacag acttattata
     4801 cagttcatcc aaaggagttg tatcaccctg aagaagtatt ttgatctgcc tgctcacaac
     4861 tcattcatga tctcggtctt ttttctttcg tactccacat cagagatcag attttggtcg
     4921 cgcatttcct ttagttcagc catgcgttca agcggagatt ttttcttttt gcccctggct
     4981 ggcttgtcgg cctgtttcag gtccttcgcc aacgagccga aatctgcagg gttgaacaac
     5041 gccttccaat agcggatgca ggccgccatg aaaattccca gcgagaagag caggccggtc
     5101 gtaaaaacgg ccagcagggt gatctccctg ccatcgcagt agatattgtg ctgccagccc
     5161 acctgtccgg gcttgtagga ataacgcgtc gtttcgatct tgaaatctcc cgaacacagc
     5221 aacggcttgg aaacgtaatg cagttttgga aagatcgccc cgacagccgc agaaaaaatg
     5281 gtgaacccga tgaacaggct gacgccaaac aaaattccga tcaagatcca gttcggtttt
     5341 ttcattattt ttctccgtag cggtaaaact atttctcggc cttatccagg tccttgccga
     5401 tatcgcgctg gaaatcccag gtgagataat cgaggttgga cggcagtgac aattccagcg
     5461 tgtacaggta ttgcaattcc aacttcacat tgcgcggacg gcattccagc aaatgtccgc
     5521 cgctggattt atcgtcggtg agaaaatgca gatgaacacc gggcacgctc agggaagcca
     5581 tgaacgaggg tgtgtaaaat ccgaccatcg tgccatgcac atcgttaaat tcaaaagtcg
     5641 gctgttcctt cgccacttcc accagcggac ggtaattttc ctgtttcggc accgagcgtg
     5701 ttttgacgta cccaaattca cccgtgatgc gaaacgcata aaagatattg ggtgaaggca
     5761 tcaactccac catccatttt agaaaatcca cataggaaac ctcgcgttcg agggtctctt
     5821 cactgaaagg ttgaaaattg gtcacacaag cgaagggcgt acaaacatca tcatctgtga
     5881 tctgattcac cctgccatca gacccaacgc ggaaaatctc accgtccacc atcatcatct
     5941 cgccatcgag atcgttgaaa gtgccgaggc caaaatcgcc gtgttttttg atctcgtaaa
     6001 aagggatgtt ctgttcgtag atgccttcca ccagggaatt gaccggcgcg cacaaataca
     6061 ccttgttatc ggcttttcgt tttctaattc tccgcagggt tgtccgttcc attttgcagt
     6121 ctcctaaaat gtgatgaagg cgattataca cttggtacaa atcaacgtca ggggagtggc
     6181 taaaaggatg ggctaacgac tcttccacca cagagatcac ggagttcaca gagattttca
     6241 aaag
//
LOCUS       DAOQJC010000179         6233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_7088_length_6233_cov_10.511169, whole genome shotgun sequence.
ACCESSION   DAOQJC010000179 DAOQJC010000000
VERSION     DAOQJC010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7088_length_6233_cov_10.511169"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PLE39_17850"
     CDS             <1..569
                     /locus_tag="PLE39_17850"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNE70253.1"
                     /translation="YPTFADVPMNHWAWAYIESIYAAGITGGCGTSPLIYCPSDPVTR
                     AQMAIFILRGVHGKAYVPPPASGTVFIDIPTDYWAADWIEQLAEEGITVGCGDGNYCP
                     ENAITRAEMSVFLLRSKLGSTHTPPPATGTTFADVPANYWAASWIEQLALMEITSGCG
                     DGNFCPEGQVTRDQMAVFIQRMFNFPLP"
     gene            696..2189
                     /locus_tag="PLE39_17855"
     CDS             696..2189
                     /locus_tag="PLE39_17855"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE70254.1"
                     /translation="MQALTKKLPFWLKLLYGSGDWGLSSIGMMRSIFYALYLTDVVGI
                     EPRLASIGALAGIVWDAINDPLIGMISDRIQTRWGRRRPFLLWFAFPFGLSFVILWSA
                     PNWESQIALTIYVTLAFMISDTLTTLVAMPYLALTPELTRDYDERTTLSSFRTVFQLL
                     AAMMVVITAPMIVDQVIHNGGTQQQGFMLAGAVFGGLSAVPLFLIGWFVHEKFQPEPQ
                     EELPFRETLRIAWKNIPFRYAAGIYMFNWSAVDMIAIIFPYFLLYWVAEGNLLAKVNI
                     LGIDLALESAFFGIMMLVCILCVPFWLWLAKTRNKREAYILGMSFWVIVQTMIFTIQP
                     GETGYLLFIGALAGVGVSAAYILPDSILPDVIEWDELRTRRRQEGIYYGIRTLIRKLT
                     GAMVIFLTLQILGWSGYQAPPDGAIQSTQSASALIAIRLMVSLLGAVILAGTILLAWS
                     YPLSREKYERIQKLLKRRRNETPPPPKPTTDPVSTTDSPEAQSAKPI"
     gene            complement(2259..3944)
                     /locus_tag="PLE39_17860"
     CDS             complement(2259..3944)
                     /locus_tag="PLE39_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNE70255.1"
                     /translation="MYDRKKLDELKNSLETWEATSLKKALSQLPERKAENEFITTSSE
                     PINRVYTPQDVAELDHETDLGLPGEYPYTRGVHPTLHRSKLWTMRMFAGFGTAEETNK
                     RFKYLLEQGQTGLSIAFDLATLMGYDTDQPEALGEFGKCGVAISSLKDMEILLEGIPL
                     DKVSSSMTINSPAAIIWAMYIAAAENKGVRADQLRGTTQNDILKEFIAQKEFIFPPEP
                     SMRLVVDTMEFGAQKVPQWNTISISGYHIREAGSTAAQELAFTLADGLEYVRWGIARG
                     MDVDDFAPRLSFFFNAHNDFFEEIAKYRAARRIWAREMRETFKAKNPRSWLLRFHTQT
                     AGVSLTAQQPENNVVRVAIQALAAVLGGTQSLHTNSLDEALALPSEHAVTIALRTQQI
                     IAEESGVTNTIDPLGGSFFVEAQTDRMEKQVYDYFRRIEELGGVIPSIEKGFFQSEIA
                     DAAYRYQREIDLGVRKIVGVNAYAEDKPYTIPILKMDPNGYTRQVGRLNEVRKTRDSG
                     RVGQALDRLRIACQGTENTMPHIMECVHAYATLGEIVAVMKETFGVYEELSVI"
     gene            4076..4603
                     /locus_tag="PLE39_17865"
     CDS             4076..4603
                     /locus_tag="PLE39_17865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HNE70256.1"
                     /translation="MPRFEIDIDPCDHITADAIGAPGQRVFYLQAYQDQRTITILIEK
                     AQLQSLAIGIEQFLSQINQQNPNLEEARGEFSEDQMRIHPPVDPLFRAGEIGLGYDKE
                     RDRVVIFTKELLTEQDDPEAAAQIRFWASRTQMRKLARWGADVVSRGRPTCPQCGQPE
                     EPGGHFCPKKNGHLS"
     gene            4659..5369
                     /locus_tag="PLE39_17870"
     CDS             4659..5369
                     /locus_tag="PLE39_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HNE70257.1"
                     /translation="MLPHPHQEIQAALTHGDYELKGQFMLGSNYTFFVDVQHEGKIFK
                     AVYKPSKGEQPLWDFPDNTLALREVAAWQLSEALGFHIVPFTTYREDGPYGPGSLQQY
                     IEYNIEHHYFNFTPEERETLRPVVLFDILANNADRKGSHVFFEEGTHKLYGIDHGICF
                     HQENKLRTVIWDFSGQQIPDDLLAPLAEAENWPGLFEQYLSPGEIRALQRRARTLLKS
                     GLFPLRPVDRRAFPYPPI"
     gene            complement(5415..5546)
                     /locus_tag="PLE39_17875"
     CDS             complement(5415..5546)
                     /locus_tag="PLE39_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70258.1"
                     /translation="MNTGLFIIVAAAFVLYGLTRTTESILDVRQAERDNGEDVKIYL"
     gene            5812..>6233
                     /locus_tag="PLE39_17880"
     CDS             5812..>6233
                     /locus_tag="PLE39_17880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNE70259.1"
                     /translation="MKHYKLALIGFGNVARALARLLVRKQSLLEQQGITFSFTGISTG
                     RHGFAVNPDGLDIQKALELTESGQSISSLSTTPVADSLAVIKNSSANVMFENSPVNTE
                     TGQPALDHMLTALNLGMHAITANKGPVVHGFSELNALA"
BASE COUNT         1543 a         1871 c         1378 g         1441 t
ORIGIN      
        1 actatcccac ctttgcagat gttcccatga atcactgggc ctgggcgtat attgaatcga
       61 tctatgctgc gggaattacg ggtggctgcg gaacgtcacc gctgatctac tgcccaagcg
      121 atcccgtcac acgtgcgcag atggcaatct ttattctgcg tggagtccat ggcaaggcat
      181 atgtcccgcc gccagccagc ggaaccgtgt tcattgatat tcccaccgat tactgggccg
      241 cagactggat cgagcaactc gctgaagaag gaatcacggt cggttgtggt gatggaaatt
      301 actgtccaga aaatgccatt acccgggctg aaatgtcggt cttcctgctg cgctcaaaac
      361 tgggcagcac ccacacaccg ccgcctgcca ccggcaccac cttcgcagat gtccccgcca
      421 attactgggc agccagctgg atcgaacagc ttgccttaat ggagatcacc agcggttgtg
      481 gtgacggaaa cttctgtccg gaagggcagg tcacccgcga ccagatggca gtcttcatcc
      541 aacgcatgtt caacttcccg ctgccgtaac cgcctttccc cactatcatt aaataaacca
      601 gccgcaggta atctgcggct ggtttattta aactttatgc tacactatcc ccatccacgc
      661 atctcaaaat cccactcggt gtttattggt acattatgca agccctgaca aaaaaacttc
      721 ccttctggct taaactctta tacggctccg gcgactgggg cctttccagc atcggtatga
      781 tgcgctccat tttctacgca ctctacctca ctgatgtggt tgggattgag ccgcgtctcg
      841 cctccattgg cgcattggcg ggcatcgtct gggatgcgat caacgaccct ctgatcggca
      901 tgatcagcga ccgcattcaa acacgctggg gcagaaggcg ccccttccta ttgtggtttg
      961 cctttccctt cggcttaagt ttcgtcatct tatggtcagc ccccaactgg gaaagccaga
     1021 tcgctttaac gatctacgtc acgctcgcct tcatgatctc cgacacgctc accacgctcg
     1081 tagccatgcc ctacctggct ctcacaccgg aactcacccg cgattacgac gaacgcacca
     1141 ccctctccag cttccgaact gtttttcaat tgcttgccgc aatgatggtc gtcatcaccg
     1201 ctccgatgat cgttgaccag gtcattcaca acggcggcac acaacagcaa ggcttcatgc
     1261 ttgcaggcgc cgttttcggc gggttaagcg ccgttccgct ttttcttatc ggctggttcg
     1321 tgcatgagaa attccagcca gagccgcagg aagaactccc ctttcgtgaa accctccgca
     1381 ttgcatggaa gaacatcccc ttccgttacg ctgcaggcat ttacatgttc aactggtcgg
     1441 cagtggatat gatcgccatc atcttcccct actttttgct ctactgggtc gccgaaggca
     1501 acctgcttgc caaggtcaac atcctcggca tagatctcgc acttgaatcc gccttctttg
     1561 gcatcatgat gctcgtatgc atcctctgcg tccccttttg gctatggctc gcaaaaaccc
     1621 gcaacaaacg cgaagcctat atcctcggca tgtccttctg ggtcatcgtg caaaccatga
     1681 tcttcaccat ccaaccgggc gaaacaggtt acctgctttt catcggcgcc ctggccgggg
     1741 tcggtgtttc agccgcctac atattacccg actcgatcct gcccgacgtg atcgaatggg
     1801 acgaactgcg cacacgccgc agacaggaag gcatctacta cggcattcgc accctcatcc
     1861 gcaagttgac cggcgcaatg gtcatcttcc tcaccctgca aatactcggc tggtccggat
     1921 atcaagcccc gccagatggg gccatccaat ccacgcaatc tgcttctgcc ctgatcgcaa
     1981 ttcgattaat ggtctccctg ctcggcgccg tcatccttgc aggcacgatc ctgctcgcat
     2041 ggtcatatcc gctctcacgc gaaaaatacg agcggattca aaaactctta aagcggcgca
     2101 ggaatgaaac cccgcctcca cccaaaccga caactgatcc tgtttcaaca acagattctc
     2161 ccgaagcgca aagcgccaaa cccatttaac gacacccacc caaacaaaaa gcggacagct
     2221 tttgctgtcc gctttttgtt ttcattgaat cagtctattt aaatcactga caactcttca
     2281 tacacaccga aggtttcctt catcactgcc acgatctcac ccagggtggc atacgcatgg
     2341 acacattcca tgatatgagg cattgtattc tcggtaccct gacatgcaat tcgcagccga
     2401 tccaaagcct gccccacacg cccgctatca cgcgtcttac gaacctcatt caagcgcccc
     2461 acctgacggg tataaccatt cggatccatc ttaagaatgg gaatggtata tggcttatcc
     2521 tcagcatagg cattcacacc gacgatcttg cgcactccca gatcgatctc acgctgatag
     2581 cgatacgctg catctgcgat ttcgctctgg aagaagccct tttcaatgga tgggatcacc
     2641 ccgcccaatt cctcgatcct acggaaataa tcataaacct gtttttccat tcggtcggtt
     2701 tgcgcctcta caaagaaact accgcccaat ggatcgatgg tatttgtcac gcccgattcc
     2761 tctgcaatga tctgctgggt acgcaaggcg atcgtcaccg catgttccga aggaagcgcc
     2821 aaggcctcat ccaatgaatt ggtatgcagg ctttgtgtgc cgcccaacac tgccgccaaa
     2881 gcctgaattg ccacacgaac gacattattc tctggctgtt gtgccgtcaa cgaaactccg
     2941 gccgtctgtg tatggaaacg cagcaaccac gaacgcggat tcttcgcctt gaaggtctca
     3001 cgcatctcac gtgcccaaat ccgtcgtgcc gcacgatact tggcgatctc ttcaaagaaa
     3061 tcgttgtgcg cgttaaagaa gaacgacaac cgcggcgcaa aatcgtccac atccatacca
     3121 cgggcaatgc cccaacgcac atactcgaga ccatcagcca gggtaaaggc cagttcctgt
     3181 gcagcggttg aacccgcctc acggatatgg tacccgctga tcgaaatcgt attccactgc
     3241 ggcacctttt gagcgccgaa ctccatcgta tccaccacca gcctcatcga aggctcgggc
     3301 ggaaagataa attccttttg tgcaatgaat tccttgagga tgtcgttttg cgtggtgccg
     3361 cgcaactgat cggcgcgcac tcccttgttc tcagcagcag cgatatacat cgcccaaatg
     3421 atcgcagcag gtgaattgat ggtcatgctc gaagagacct tatccaacgg aatgccttcg
     3481 agcaaaattt ccatatcctt caaggatgag atcgccacgc cgcattttcc aaattcaccc
     3541 aacgcctcag gctggtcggt atcatagccc attaaagttg ccaggtcgaa ggcaatgctc
     3601 aatcctgttt gaccctgttc cagtaagtat ttgaagcgtt tgttcgtttc ctccgcagtg
     3661 ccgaaccctg caaacattcg catggtccac aatttgctgc ggtgcagagt cgggtgaacc
     3721 ccgcgcgtgt aggggtattc acccggcaat ccaagatcgg tttcatgatc caattcagcc
     3781 acatcctgag gcgtatagac cctgttgatg ggttctgaag aggtggtgat aaattcgttt
     3841 tcagccttgc gctcagggag ctgagacaac gccttcttca gggaagtcgc ctcccatgtt
     3901 tcaagcgagt ttttcaattc atccaatttt tttctatcgt acatggcaca ctcctttggc
     3961 gggatgtacg gattatacat gaggcgtagt ctggtatact accaaaaata cacttttaaa
     4021 aaaaacagac aaacaggtgg tttcatctgt ccaatgattt tacaaaaggt aacatatgcc
     4081 tcgtttcgaa attgacatag atccatgtga tcacatcact gccgatgcca tcggcgcccc
     4141 gggccaacgc gtcttttatc tgcaagccta ccaggaccaa cgcacgatca ccatcctgat
     4201 tgaaaaagcc cagttgcaat cgctcgccat cggaatcgaa caattcctct cacagatcaa
     4261 ccagcaaaac cccaacctcg aagaggcgcg gggtgaattc tctgaagatc aaatgcgcat
     4321 ccacccaccg gtagacccgc tcttccgtgc aggtgagatc gggcttggct acgataaaga
     4381 acgcgaccgc gtcgtgatct tcacaaaaga attgctcacc gaacaggatg accccgaagc
     4441 cgcagcacag atccgctttt gggccagccg cacccaaatg cgcaaactcg cccgctgggg
     4501 cgcagatgtg gtcagccgtg gacgccccac ctgcccgcaa tgcggccaac ctgaagagcc
     4561 cggcgggcat ttctgcccaa agaaaaacgg gcacttgagt taagttgaac gttgctggtt
     4621 gaaagtttca accagcaacc tgtaaccttc agccatttat gctcccacat ccccatcaag
     4681 aaattcaagc cgccctcacc cacggtgatt acgaactcaa aggccagttc atgctgggct
     4741 caaattacac attctttgtg gatgtgcagc atgaaggcaa aatattcaaa gctgtgtaca
     4801 aacccagcaa aggcgagcag ccgctttggg atttccccga taatacgctg gccctgcgtg
     4861 aagtagccgc ctggcaacta agcgaagcgc tcggctttca catcgttccg ttcaccacct
     4921 atcgcgaaga tggcccatac ggccccggtt ccctgcaaca atacatcgaa tacaacatcg
     4981 aacatcatta cttcaacttc acccccgaag agcgtgaaac cctgcggccc gttgtgctat
     5041 tcgatattct cgccaacaat gccgaccgca aaggcagcca tgtttttttt gaagaaggca
     5101 cacacaaact atacgggatc gatcatggaa tttgctttca ccaggaaaac aaactacgca
     5161 ccgtaatctg ggatttctcc gggcaacaga ttccagacga cttgcttgcg cctctcgccg
     5221 aagccgaaaa ctggcctggc ctctttgagc agtatctcag cccgggcgag atccgcgcac
     5281 tccaacgccg cgcacggaca ttgctaaagt cagggttgtt cccgttacga cccgttgatc
     5341 gaagagcgtt tccgtatccc ccgatctaat caaaaaggga cactgaatct tcagtgtccc
     5401 tttttttcgt ccatttacaa ataaatcttt acgtcttcgc cgttatctct ttcggcttga
     5461 cgaacatcaa gaatggactc ggtcgtgcgg gtaagcccgt ataaaacgaa ggctgctgca
     5521 acaatgatga acaatccggt attcattttg attctccttt ctatttccat gcacaaacac
     5581 ccggaagaac agacgcaaaa ctaaagattt gcagacaaaa taggtcaaca aatttatgat
     5641 aaaaagactt ccgctaagct tcgtgaaggg atgcacaagt attataaccc aattcagcat
     5701 tcccatacca gaaaaaaccc ttggcggata aaataacgcc cacaaaaatg atttgaaacc
     5761 cgaacacggc tctttcaaca attcacgaaa taaacgaaaa cggagacctc aatgaaacac
     5821 tacaaactcg cactgatcgg cttcggcaac gtagcccgtg cccttgcccg attgctcgtc
     5881 cgcaaacaaa gcttgcttga acaacaggga atcaccttct cattcacggg catttccacc
     5941 ggcaggcacg gattcgccgt caaccccgat ggcctcgaca ttcaaaaggc gctggaactc
     6001 accgaaagcg gacaatccat ttcgtcgctt tccaccactc ctgtggccga ttcgctggcg
     6061 gtcatcaaga actcatctgc aaatgtcatg ttcgaaaact cacccgtcaa cactgaaacc
     6121 ggccaacccg ccctggacca tatgctgact gctttgaacc tgggaatgca cgccatcacc
     6181 gcaaacaagg gacctgttgt acatggtttc agcgaattga acgccctggc aga
//
LOCUS       DAOQJC010000180         6116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_7289_length_6116_cov_10.794918, whole genome shotgun sequence.
ACCESSION   DAOQJC010000180 DAOQJC010000000
VERSION     DAOQJC010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7289_length_6116_cov_10.794918"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..869
                     /locus_tag="PLE39_17885"
     CDS             315..869
                     /locus_tag="PLE39_17885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE70260.1"
                     /translation="MGRKDAPPNLGDALIQDAQHGEPAALAAIYDAYALRIHRYFYSR
                     VENAADAEDLAAQTFLSVIEALPHYQHRGQFTAWIFQVARSKAMDFFRRTHSTVQHEA
                     KMVGSAFDDTLEQVIRGQDVERLRHLIRLLDDDERELLRLRFVVELSYVEIAELLGRK
                     EDAVRKSVNRTLERLHLLMEAKHV"
     gene            862..2364
                     /locus_tag="PLE39_17890"
     CDS             862..2364
                     /locus_tag="PLE39_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70261.1"
                     /translation="MSKPNYPPEFETQVRQAMEVSEPKPETLDALRKQFIARGTSSLK
                     TDLQPDPAPNPFRPEKESNKMNRKPFRFSPRLAWSVALLILASLLVIAFSSPKVVTAL
                     RRLLGYVPGVGVVEQSPSLRMLAEPVTVTRESVTLTIDQAVLTNEKTVVVYSYVTPLY
                     NSDEGFFMDGPMGIPALVLPDGTRLEIVIGRQVSTMDCSECGDIRYAMEFPPIPADVQ
                     EATLEIPNLVGLQNGLTPLNWVIDLKFAPADPSTIIPVIEYQPTATPVSATSVSTTQT
                     PETYGAILVLDKSASLPDGYILYGTITWTDPIIPQYGLSATLAGIKDANGNDIPFEYA
                     DAEAYPQQGELREYWAYKIPQLDFAAPLTLSFAVNAWTPLTDAPSFTFDPGPNPQLGQ
                     KWEINQDVVVKDEIVHVLSVEQAGIEEGFFMFTMQSDSNIVGASITDLQHPPMGGGGG
                     GGGVPVVKLPFFVGFGYQTPIPTGPLTLTFQSVNVLLQGDWSVTWSPAAP"
     gene            complement(2371..3021)
                     /locus_tag="PLE39_17895"
     CDS             complement(2371..3021)
                     /locus_tag="PLE39_17895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE70262.1"
                     /translation="MPESIRILIVDDHAVVRKGLAMVLRLESDLEVVGEAENGRIGLE
                     AAKNLNPDIVLVDLVMPEMDGQEMALALRKSNPKTKVMMLTGTEVDDRVFDLVAAGIE
                     GYVLKNIEPGELVRAIHAVVHGEAYLHPDVMKKVVGRMQPHHTQPIVLTPREIEILEW
                     MASSNTYKQIAGHLNVSEETIRSHAKNILEKMKQPNRAQAVLAAMRVGLIKLDESK"
     gene            complement(3014..4678)
                     /locus_tag="PLE39_17900"
     CDS             complement(3014..4678)
                     /locus_tag="PLE39_17900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNE70263.1"
                     /translation="MITRVESFFRSNRVFVFLVIFRWASLLPALFTLGTTDALIHPLL
                     VLALALAINGIVALFNRPLNRMVVEKPALLSIDLLFSAFILAISGGSHSPYFLYALSP
                     LLAGAFFFQLRGALTTSLLFTILYSLAITYPAQPPADPTVLATQFAGIWVLPILFAYP
                     STLLKSVNQATEELSAAHRQLEIIHDLTVLLQAAPDLISVQERVLGAVVTDLGFSKAI
                     VAMVDPAHEEIGGWLIYPPENTFPQTKPLPLKSGSGEIIKALLNRNNLTTTGQTLIQD
                     PQLNHWLNQNQWFIVPLYLREHPVGVLLVEESGLTREKESTLMTVANQAALSLGTTIL
                     CIDRARRLAVETERNRIARDIHDTVAQSLFGIVYSLDACITMLPQQADDVKKELIDLR
                     TLANTAHDEVRRSIFDLWPSALNIELFMADLTNYVSGCCRPRSFSIIFNNHGDFNSLS
                     SGMRRTVYRMAQEALANSARHSGAQSARLCLNVTSDEVYLVVSDEGKGFDPSVALSRS
                     QNREHFGLHGIQERARAMGGDCEVISQPNAGARIMIQLPNNGHHHA"
     gene            4808..5047
                     /locus_tag="PLE39_17905"
     CDS             4808..5047
                     /locus_tag="PLE39_17905"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNE70264.1"
                     /translation="MTDVIKEDQVLDCSGMLCPMPVVKTSKAMKELQSGQVLKMVATD
                     PGAPPDMEAWSRQTGNELLRSMTEGGKFIFYLRKK"
     gene            5079..5603
                     /locus_tag="PLE39_17910"
     CDS             5079..5603
                     /locus_tag="PLE39_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNE70265.1"
                     /translation="MSKSDPNAPKRLALIASKGTLDWAYPPFILASAAGAMGWEVGIF
                     FTFYGLTLLKKDLSASVSPLGNPAMPMKMPFGPEGFQNIAWPMPNLLMANVPGFEALA
                     TNLMKQTFKNKGVASIEELRDMCIDLDVKMIGCQMTMDVFGFKKEDFIKESEVAGAAM
                     FLEYAADADVQLFI"
     gene            5660..5998
                     /locus_tag="PLE39_17915"
     CDS             5660..5998
                     /locus_tag="PLE39_17915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE70266.1"
                     /translation="MDKIALQSEQKGNVVVVSVSGRVDSDTAPMFEAELTKAVSGNAK
                     LVLDLKGVEYMSSAGLRAIVKAAQAVEPKGGAVKMAAVPELVTSIFYTVGLNQKVAEY
                     KSVEEAMASF"
BASE COUNT         1471 a         1462 c         1676 g         1507 t
ORIGIN      
        1 gatttggatg aactttcaaa ctgggaggct tattcgctct gaaagtattt ggcaatttga
       61 agatggcacg gaacagcttg aaaccttctt tgttctccgt attcttgaaa aactggattc
      121 accccccaga gaggttttga atatttttga aaaaatcatc ctgccgtagt aaaagatggc
      181 tggaggaggg aatttttgcc gaggcaacag acaggggctg aaatctggat acccattttg
      241 tccgcttttt gaaggttttg cgtttaatat tatagagttt cctttgcaaa gggtatcgaa
      301 tgtcaaatca aaacatgggt cgaaaagatg cgcctcccaa cctgggagat gcgctgatac
      361 aggatgcaca gcacggcgaa cccgccgcac tggcggcgat ttatgacgcg tacgcgctcc
      421 gcatccaccg ttatttttac agccgtgtgg aaaatgcggc tgatgctgaa gaccttgccg
      481 cacagacctt cctcagtgtc attgaggcct tgccgcacta tcaacaccgc ggtcaattca
      541 ccgcgtggat ctttcaagtc gctcgaagca aggcaatgga ttttttccgc cgcacccact
      601 caacggtcca gcatgaagcg aagatggtgg gcagtgcttt cgatgacaca ctggaacagg
      661 tcattcgagg tcaggatgtc gaacgtcttc gtcacctcat ccgacttctg gatgacgacg
      721 agcgcgaatt gctgcgtctg cgttttgtgg tcgaactctc gtatgttgag atcgccgaac
      781 tgcttggacg aaaagaagat gccgtcagaa aatctgtgaa ccgcacactc gaacggttgc
      841 acctgctgat ggaggccaaa catgtctaag cccaattatc cgcccgaatt tgaaacacag
      901 gtgcggcagg cgatggaagt gtctgaacca aaacccgaaa cattggacgc cctgcgcaaa
      961 cagttcattg ctcgtgggac ctcctcgctc aaaacggacc ttcagcctga tcctgctccc
     1021 aatccttttc gacccgaaaa ggaatcgaac aaaatgaatc gcaaaccatt tcgcttttcc
     1081 ccgcgccttg catggagcgt ggcgctgctc atcctcgcaa gcctgcttgt gatcgcattc
     1141 tcatctccca aagtagtgac cgccttgcgc cgcctgctgg ggtatgtccc cggtgtgggt
     1201 gtggtggagc aaagcccatc tctgcgcatg ttggctgaac ccgtcaccgt gacgcgcgaa
     1261 agtgtgaccc tcaccattga tcaggctgtt ctgaccaatg aaaagacagt tgtcgtgtat
     1321 tcctacgtga cacctttgta taactctgat gaaggctttt ttatggatgg cccaatggga
     1381 atccctgcac tggttttgcc cgatggcacc cgactcgaaa tcgtaattgg acggcaggtg
     1441 agtacgatgg attgttctga gtgtggcgac atccgctatg cgatggagtt tccgcccatc
     1501 cccgctgatg ttcaagaggc gacccttgag atacccaacc tggttgggct gcaaaacggc
     1561 ctgacaccct tgaattgggt cattgatctg aagtttgcgc ctgccgaccc atccaccatc
     1621 atccctgtca tcgaatacca gccgacagcg acgcccgtca gcgcgacgag tgtttcaaca
     1681 acccagacgc ccgaaaccta tggcgcaatc cttgtgcttg ataaatccgc gtcgcttcct
     1741 gatggatata ttttgtatgg caccatcaca tggaccgatc ccatcatccc ccaatatggt
     1801 ttgtctgcca ccctggcagg catcaaggat gcgaacggaa atgacatccc cttcgaatat
     1861 gccgatgcgg aagcataccc acagcaggga gaattacgcg aatattgggc gtacaagata
     1921 ccgcagttgg atttcgccgc accgttgact ttatcttttg cagtgaacgc atggacccct
     1981 ttgacggatg caccctcttt cacctttgac ccggggccaa atccgcagtt ggggcagaaa
     2041 tgggagatca atcaggatgt ggtggttaag gatgaaattg tccatgtcct ctctgtagaa
     2101 caggcaggaa ttgaagaagg atttttcatg ttcaccatgc agtctgattc gaacattgtc
     2161 ggcgcgagca tcactgacct ccagcatccg cccatgggcg gtggaggtgg tgggggaggt
     2221 gtgcctgttg taaaactgcc tttctttgtt ggttttggtt atcaaacccc catcccaacc
     2281 gggccgctta cactgacttt ccaatcggtg aatgtgcttc ttcagggcga ttggtctgtc
     2341 acctggtcgc ctgcggcacc gtaaaaacgg ctatttgctc tcgtccaatt tgatcaaccc
     2401 aacccgcatg gcggccaaaa cggcctgcgc gcggttgggt tgtttcattt tttcgaggat
     2461 gttcttggca tggcttcgga tggtctcttc actgacattt agatgccccg cgatctgttt
     2521 ataagtgttc gaagaagcca tccattcaag aatctcgatc tcgcgcgggg tcaacacgat
     2581 gggttgtgta tggtgcggct gcatgcggcc caccactttc ttcatcacat cagggtgcaa
     2641 atatgcttcg ccatgcacca ccgcatgaat ggcgcgcacc aattcaccag gttcaatatt
     2701 tttcagcaca tagccttcaa tgcccgccgc aaccaggtca aaaacacggt cgtccacttc
     2761 ggtgcctgtc agcatcatta cctttgtttt cgggttggat tttcgcaaag cgagagccat
     2821 ttcctgacca tccatctcag gcatgaccag atctacaagg acgatatctg gatttagatt
     2881 cttcgccgcc tccagcccga tccgaccatt ttccgcttcg cctacgacct ccaaatctga
     2941 ttccagacga aggaccattg ccaacccttt gcggaccacg gcatggtcat ctacgatgag
     3001 aatgcggatc gactcaggca tggtggtgtc cattgttggg gagttggatc atgatgcgcg
     3061 caccagcgtt gggctgcgag atgacttcgc aatcaccacc catggcgcgg gcacgttcct
     3121 gaattccgtg cagaccaaaa tgctcgcggt tttggcttcg tgaaagcgcc acagatgggt
     3181 cgaagccttt gccttcatct gaaacaacga gatacacttc gtcgctggtt acattcaagc
     3241 acaatctggc tgattgcgcc cccgagtgcc gtgcagaatt tgccagcgcc tcctgtgcca
     3301 ttcggtaaac tgtccgacgc atgcctgaag agagggaatt gaaatcgccg tggttgttga
     3361 agataatgct gaaagaacgt gggcggcagc agccactgac gtaattcgtc aggtctgcca
     3421 tgaagagctc gatgttcaag gcagagggcc acaggtcaaa gatcgagcgg cggacttcgt
     3481 cgtgggcggt gttggcgagg gtacgcaggt cgatcaactc tttttttacg tcatcggctt
     3541 gttggggcag catggtgatg caggcatcca gcgaataaac gatgccaaag agcgattgag
     3601 cgaccgtatc atgaatatcg cgggcgatgc ggttgcgttc ggtttcgacg gcgaggcgtc
     3661 gggcgcggtc aatgcataag atggtggtac caagcgacag ggcggcttga ttcgccacag
     3721 tcatcagcgt ggattctttt tctctggtaa gtccagattc ttccacgagc aatactccga
     3781 cggggtgttc cctcaaataa agcggtacga tgaaccattg gttttggtta agccagtggt
     3841 tgagttgggg gtcttgaatc agggtctggc ctgttgtggt gaggttattg cgattgagaa
     3901 gggctttgat gatctcgccg cttcctgatt tgagggggag gggtttggtt tgcggaaagg
     3961 tgttttcagg tgggtagatg agccaaccgc cgatttcttc atgtgcgggg tctaccatgg
     4021 cgacaatggc ttttgagaaa ccaaggtcgg tcaccactgc gccgaggaca cgttcctgca
     4081 ctgagatcag gtctggtgcg gcttgcagca acactgtgag gtcatgaatg atctccagtt
     4141 gacggtgtgc tgctgagagt tcctcagtgg cctggttgac ggatttaagc agcgtggagg
     4201 gatatgcaaa gaggatgggc aaaacccaaa tgcctgcaaa ttgagtagcc aatacggtgg
     4261 ggtctgcagg cggttgggcg gggtaggtga tggcaaggga atataaaatt gtaaaaagca
     4321 gggatgtggt cagtgcgccg cgaagctgaa aaaagaatgc gccagccagt aatgggctga
     4381 gcgcatacag aaagtaaggg ctgtgtgatc cgccgctgat ggcgaggatg aaggcggaga
     4441 agagcaggtc aatgctgagg agggcgggtt tttctaccac catcctgttc aatgggcggt
     4501 tgaacagagc tacgatgccg ttgatggcaa gcgcgagtgc aagcaccaac aacggatgga
     4561 tcaatgcgtc tgttgttcca agcgtaaaca acgctggtaa cagggaagcc cagcgaaaga
     4621 tgacaagaaa tacaaaaacg cggtttgatc ggaaaaacga ttcgactctt gtaatcataa
     4681 aagttgtaga attgattata tccgaattgg ccatctcccc cgcgcggggg agataaaatc
     4741 ccttgcgtgt gggatggcat atcggcggtc aattcattat gatttatgag ttccaaagga
     4801 ggcacaaatg acagatgtaa ttaaggaaga tcaggttttg gattgctctg gaatgctttg
     4861 ccccatgcct gtggtgaaga catcaaaagc aatgaaggaa ttgcaatctg gacaggtttt
     4921 gaagatggtt gccaccgacc ctggtgctcc ccccgatatg gaagcctgga gccgccagac
     4981 aggcaacgaa ctactgcgtt ccatgaccga aggcggtaaa ttcatttttt acctgcgcaa
     5041 gaaatagaac ttgttccaaa ttcttaatag aggtccctat gtcaaaatct gatccaaatg
     5101 ctccaaagag acttgccttg atcgccagca agggaacgct ggattgggct taccctccat
     5161 tcatccttgc cagtgcggca ggggcgatgg ggtgggaggt gggtattttc tttacctttt
     5221 atggtttgac tttgcttaag aaggatctta gcgcatctgt ctcaccgttg gggaacccgg
     5281 ccatgccgat gaagatgccc tttggtcccg aaggttttca gaacattgcc tggcccatgc
     5341 cgaatttgtt gatggcgaat gtgcctgggt tcgaagcact ggcgaccaac ctgatgaagc
     5401 aaaccttcaa gaataagggc gtcgcttcga ttgaagaact tcgcgatatg tgcattgatc
     5461 ttgatgtgaa gatgatcggc tgccagatga cgatggatgt ctttggcttc aagaaggaag
     5521 attttattaa ggaaagtgaa gtggctggcg cagccatgtt ccttgagtac gccgccgatg
     5581 cggacgttca attgtttatc tagtaggttg ccctgtaggt cacgcataag gcgtgaccta
     5641 cttaaaaaag gataaatgca tggataagat cgctcttcaa tctgaacaaa aaggaaatgt
     5701 tgttgtggta tctgtttctg gacgggtgga ttctgataca gcccccatgt ttgaagccga
     5761 attaaccaaa gcggtaagtg ggaatgcaaa gcttgttctg gatctcaagg gcgtggaata
     5821 tatgtcgtct gcagggcttc gcgcgatcgt aaaagcggct caggctgtgg agccgaaggg
     5881 tggagcggta aaaatggcag ctgtgcctga gttggttacc tccatttttt ataccgtcgg
     5941 tttaaaccag aaagtcgctg aatataagtc tgttgaagaa gcaatggcta gtttctaatt
     6001 tcagataaat aggagagggc agtatgagcg tttggaagac gaatgatcct gaaacaaaga
     6061 gaattttgta cgttcagact cacggggaga aggatcctga acgaacggca acgcct
//
LOCUS       DAOQJC010000181         6088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_7335_length_6088_cov_8.724018, whole genome shotgun sequence.
ACCESSION   DAOQJC010000181 DAOQJC010000000
VERSION     DAOQJC010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7335_length_6088_cov_8.724018"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..6007)
                     /locus_tag="PLE39_17920"
     CDS             complement(86..6007)
                     /locus_tag="PLE39_17920"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNE70267.1"
                     /translation="MKNQKTLRIIGVIAIFALAFVLTKSIFFNNAPLSIEAEPTGPQV
                     IAQNPIEGQRLTLEPSIQFTFDRDMDKAATEKAFSLLSNGEPVPGQLTWLDTRTFSFS
                     PDSKLTPAAEYMAVITTSAIAKDGTSPEEEIKIEFMTVETLSVSQVFPAPDTQEVDLN
                     STITVIFNHPVVPLNIVEEQDKLPQPLKFTPAINGKGEWVNSSVYVFQPEELLTSGTS
                     YQVRVDPKLQDTLENTLGESYIWQFDTRKPIIYNFALKDGEQNPPGDVYNMRLDQSFI
                     VSFEQPMDRKSTEAAFKLINRETKAVFPVSFSWNDESTELVIEPKSNFKIASFYELTL
                     NDTAQAVDSGTLREGLNVGLATVPLPSITSVYPPKDSDNADYRSTIEIQFASQMDLES
                     LKGRVQITPALQGGEDDWYYNSYNHTLNIYGLEPATRYAVRILPGMADIYGNTIQDEL
                     SYAFYNGDYATVANIAMPWNNPLIYRAHGTQEVFLEYQNINEATLAIYPISFTEFSRF
                     NSGNAVPAQYDPNEKPIREWKVAAGERNKLERELFNFQDKNGASLDAGYYFIGLKVKP
                     FKYNDNFFQAFTFIVANDNITLKATPTEGFAWVTDLESGAPRSNVAVTFYDDKFQPLG
                     KVNTDKNGIAHLKNIKSVNYAQIQDSSDDFGFTALYWGSGVSAGTFNLYGNYYSSVDK
                     LFGYLYTDRPIYRPDQEVYFKGILRQDDDLHYSHPRINQVYVVVEQWGEKIYEDYVPI
                     NDQGSFSGKVKLDADVSLGYYMVYAYVSNSPNEGPITSVGFSVAEYKKPEFEVLVSPD
                     STEVLMGEEVNFGLDAEYYSGGLLKNASVEWFIEARPYYFHPIEKYNRYSFMDWNRDM
                     YWEPETQSGNGTLAEGSGETDENGHFDIAQILESSKENEGISQTMTLSANVADVAGNT
                     VSGTGSVVVHHSEYYAGIRSEKYVATQGEAQAFNLVVLDWNSQPIADQKVTVSFVERR
                     WYSIQKQDEQGMLQWETSVKEIPVSSVEATTGADGTAQVEFTPPNGGVFKALVKVIDT
                     KGNTHQASTYLWVSSKEQIFWRQTNDRAFNLIADKDSYSPGDTAEILIAQPFQGDVYA
                     LITYERGHIYKSEVVKLNGNSTIYHLPITEEMVPAAYVSVTVISGAQNSGAPDFKMSI
                     ARLNVDTELKTLDVSVSANKESAGPGDKVTYTIETKDSKGAPVSADVSLSVVDKAVLA
                     LMPSNTIPLLSSFYATRGLGVMTALGIVSSADDFNANYRKTIPDGNKSGGGGGGEPGV
                     ITVRENFKDTAAFEAEVMTDENGIAKVDVELPENLTTWVAEARAITEDSRVGQATSEL
                     ISSKPLFVQLQTPRFFVVGDQLTVGATVFNNLETSLKVDISLDAEGVQVLSDPKQSIE
                     IEGRQQAYVTWDIVVKANVERVDLTATAISGQFTDASKPALGTLDGQGIPVYNFTAVE
                     TVGTSGMILTADSVTESIQLPTTLNIEDANLSIEISPSLAASMQSSLKYLEDYPYLCM
                     EQTISRFLPNVITMRALDAAGRPTPMRGSLNTQVNSALQRIYAKQKYDGGWNWWDGEL
                     SDPQTSAYVIYGLIEAQASGYEISETVLANGLDYLKNNLPDIKRNEASWHYNRYAFML
                     YVLARADELDAGKTNFIFENHSKLDLYGKAYLAQAMFMLDEEDPRIKTLLSDLVTATV
                     QSASGAHWEESTKDYWNWNTDTRTTAIVLNAFVQIDPQNPITANAVRWLMAHRESGHW
                     QSTQETAWSLIALTNWLIESQEYETDYKYAVGLNGNLLDEGQASAEDLTETVNLQVQI
                     EDLLKEQGNDLVITRGRGTGNLYYSAYLSTSLPVDSIQPLDQGVSLSREYFTLDDPKT
                     PITEIERGELVKARLTIVIPAAVHYIVIDDPLPAGMEVLNASLATDTAVPSFYSAQDY
                     KDRGWGWWYFSHIELRDEKITLSTDYLPAGTYVYTYIVRASTAGTFNVIPPTASEFYF
                     PDVGGRGAGSVFTVR"
BASE COUNT         1316 a         1438 c         1649 g         1685 t
ORIGIN      
        1 agaatgggcg aattcatcac atagctgaat cgattccacg cctgcggatt gtccgtgccg
       61 gggatgaggg tgtcgggctg ggtttttatc tcaccgtaaa cacactccct gcgcctcttc
      121 cgccgacatc ggggaagtag aattcggatg cagtgggtgg aatgacgttg aacgtacctg
      181 ccgtgctggc gcggacgatg taggtgtaca cgtaggttcc tgctggcaga taatcggtgg
      241 agagggttat tttttcatcg cgcaattcga tatgggagaa gtaccaccag ccccagccgc
      301 ggtctttgta atcctgtgcg gagtagaagg atggcaccgc ggtatcagtt gcgagactgg
      361 cattgaggac ttccatgcct gcgggcaggg gatcgtcaat gacgatgtaa tgtacggcgg
      421 cagggatgac gatggtcagc cgcgctttga ccaattcgcc gcgctcgatc tcggtgatgg
      481 gagtcttggg atcgtcgagg gtaaagtatt cacgggaaag gctcacaccc tgatcgagcg
      541 gttggatgga atccacaggc aggctggtgc tgagataggc gctgtaatac aggttgcctg
      601 tgccgcgtcc acgggtgatg acgaggtcat tgccctgttc cttgagcagg tcttcgatct
      661 gcacttgcag gttgacggtc tctgtcagat cctcggcgct tgcctgacct tcatcaagca
      721 ggttgccatt caagccgacg gcgtatttgt aatcggtctc atattcctgc gactcgatca
      781 gccaattggt caatgcgatc agtgaccatg ccgtttcctg cgtggattgc cagtgtccgc
      841 tttcacgatg ggccatcaac cagcggaccg catttgcagt gatggggttt tgtggatcaa
      901 tctgcacgaa ggcgttgagc acaatggcgg ttgtgcgtgt gtcggtattc cagttccagt
      961 agtccttggt ggattcctcc caatgggcgc ctgatgccga ttggacggtg gcagttacga
     1021 ggtcggaaag aagcgttttg atgcgcgggt cttcctcatc gagcatgaac atcgcctgcg
     1081 ccagataggc tttcccgtag agatccaatt ttgagtgatt ttcaaaaatg aagttggtct
     1141 tgcctgcatc gagttcatcg gcacgggcga gcacgtagag catgaaggcg tagcggttgt
     1201 agtgccacga ggcttcgttg cgtttgatgt caggcagatt gttcttcagg tagtccaggc
     1261 cgttggcgag tacggtttca gagatttcgt atccgctggc ttgcgcttcg atcaaaccat
     1321 aaatgacata tgcagaggtc tgcgggtcgc ttaattcgcc gtcccaccag ttccagccgc
     1381 cgtcgtattt ttgttttgcg taaattcttt gcagtgccga attgacctgc gtgttcaacg
     1441 agccgcgcat gggagtgggg cgtcctgcgg catccagtgc gcgcatggtg ataacgttgg
     1501 gcaggaagcg tgaaatggtc tgctccatgc acaggtatgg ataatcttca aggtatttca
     1561 aactcgattg catcgaagcg gccaacgatg gtgaaatttc aatggagaga ttcgcgtctt
     1621 cgatattcaa agtggtgggc agttggatgc tttcagttac cgagtctgcg gtgaggatca
     1681 tgcctgaagt gccgaccgtt tcgacggcgg tgaagttgta cacgggaatg ccctgcccat
     1741 cgagtgtgcc cagtgcgggt ttgctggcat cggtgaattg cccgctgatc gcggtggcgg
     1801 tcagatccac gcgttcgaca tttgctttga caacgatatc ccaggtgacg taagcctgct
     1861 gcctgccttc gatctcgatg ctttgttttg gatcagacaa aacctgcaca ccttccgcat
     1921 ccaatgagat atccaccttg agggatgttt ccaaattgtt gaagacggtc gcacccactg
     1981 ttagctgatc cccaacgacg aagaatcgcg gcgtttgcag ttgcacaaag agcggtttcg
     2041 agctgatcaa ttcactggtc gcctgaccca cacggctgtc ttcggtgatg gcacgcgctt
     2101 cagccaccca ggtggtgagg ttttcaggca attccacatc aaccttcgcg atgccgtttt
     2161 catcggtcat cacttctgct tcaaatgcgg ctgtgtcttt gaaattttca cgcacagtga
     2221 tgacgccagg ttcgccgccg ccaccgccgc cgcttttgtt gccgtcagga atggtcttgc
     2281 gataatttgc attgaagtca tcggcactgg aaacgatacc caatgccgtc atgacgccca
     2341 gtccgcgggt ggcgtaaaag ctacttaaca atgggatggt attggaaggc ataagtgcca
     2401 gcacggcctt gtccactacc gaaagcgata catctgcaga gacgggtgcg cccttgctgt
     2461 cttttgtttc gatcgtgtat gtaactttgt ctccggggcc tgctgattct ttattggcag
     2521 agactgaaac atcaagggtt ttaagttcag tatccacatt gagacgggcg atgctcattt
     2581 tgaaatcagg tgcaccgctg ttttgggctc cgctgatgac ggttaccgaa acataagccg
     2641 cggggaccat ttcctcggta atggggaggt ggtagatggt gctgttcccg tttagtttga
     2701 caacttcgct cttgtagatg tgcccacgtt cgtaggtgat gagggcatac acatccccct
     2761 gaaacggttg ggcgatgaga atttcggctg tgtcccctgg tgaatagctg tctttgtcgg
     2821 cgatgaggtt gaatgcacgg tcatttgtct gtcgccagaa tatctgctct ttgctggata
     2881 cccagagata agtggatgcc tgatgagtat ttcccttcgt gtcaatcact ttgacgaggg
     2941 ctttgaacac gcctccattg ggaggagtga attcaacttg tgcggttcca tctgcgcctg
     3001 ttgtggcttc cactgaactg accggaatct cttttacgga agtttcccat tggagcatcc
     3061 cttgttcatc ctgtttttgg atgctgtacc agcgcctttc cacaaaactg acggtcactt
     3121 tttggtcagc aatgggctgg gagttccaat cgaggacaac gaggttgaag gcctgtgcct
     3181 ccccttgcgt agcaacatac ttttccgagc gaataccagc gtaatattca ctgtgatgga
     3241 ccacaacgct ccctgtcccg ctgacggtgt ttcctgcgac atcagcgaca ttggctgaga
     3301 gggtcattgt ttgcgaaatg ccttcatttt cttttgaaga ttcgagtatt tgcgcaatgt
     3361 caaaatggcc gttctcgtcg gtttcgccgc tgccttccgc caatgtccca ttccctgatt
     3421 gggtttcagg ctcccagtac atatcgcgat tccaatccat gaaactgtag cggttgtact
     3481 tttcgatggg gtggaaataa tacggtcttg cttcgatgaa ccattccacc gaggcgtttt
     3541 tcaagagacc accagaatag tattctgcat ccagtccaaa attgacctcc tcgcccatga
     3601 ggacttcggt cgaatcgggt gagaccaaca cctcgaattc aggcttcttg tattcggcaa
     3661 cgctgaaacc aaccgatgtg atgggtccct cgtttggaga attggatacg tatgcgtaga
     3721 ccatgtaata ccccaaagaa acatcagcat caagttttac cttgccgctg aaacttccct
     3781 gatcgttgat gggtacatag tcttcgtata ttttttcgcc ccattgttca acgacaacat
     3841 atacctgatt aatgcgtgga tggctgtaat gcaggtcgtc atcctgacgt aggatgccct
     3901 tgaagtaaac ctcctgatca ggacgatata ttgggcggtc ggtgtataga tacccgaaaa
     3961 gtttatccac actgctgtaa taatttccgt acaaattgaa cgtgcccgcg ctgacgccgc
     4021 tgccccagta caaggccgta aacccaaaat catcactgct atcctgtatc tgggcgtaat
     4081 tgactgactt aatgtttttc agatgggcaa tgccgttctt gtcggtgttg actttgccca
     4141 gcggttggaa tttgtcgtcg taaaaagtga ctgccacatt actgcgtggt gcgccgcttt
     4201 ccaggtctgt cacccaggca aacccttcgg tgggtgtggc cttgagcgta atattgtcat
     4261 ttgccacaat gaatgtaaat gcctgaaaga aattgtcgtt gtatttaaaa ggttttacct
     4321 ttaagccaat aaagtaatac ccagcatcca acgaagcgcc atttttatcc tggaagttga
     4381 ataactcgcg ctcaagcttg tttcgttctc ctgcggctac cttccattcc cgtatgggtt
     4441 tttcgtttgg gtcatattgt gcagggactg cattgccgct gttaaaacgg ctgaactctg
     4501 taaaactgat cgggtaaatg gcaagggttg cctcattgat gttttgatac tcgagaaaca
     4561 cttcctgagt tccatgtgct cggtatatca aggggttgtt ccatggcatg gcaatgtttg
     4621 ccaccgtggc ataatctcca ttgtagaagg cataggaaag ttcgtcttga attgtgttgc
     4681 cgtaaatgtc agccatgcct ggaagtattc gaactgcata acgggtggca ggctcaagac
     4741 cgtaaatatt caaggtgtga ttgtagctgt tgtaatacca atcatcctcg ccgccttgca
     4801 gggcgggagt gatttgcacc cgacctttca ggctttcaag atccatttga gaagcgaatt
     4861 gaatctcaat ggtggagcgg tagtctgcat tgtctgagtc tttgggcgga taaacacttg
     4921 tgatcgaagg caatggaaca gttgccagtc ccacattcag accttctctc agcgttccac
     4981 tatccacggc ttgtgcagtg tcgttgaggg tcaattcgta gaaacttgca atcttgaagt
     5041 tggatttcgg ttcaataacc aattccgtgc tttcgtcgtt ccacgaaaaa gacacgggga
     5101 agacagcttt tgtctcgcgg ttgatgagtt tgaacgccgc ttccgtgctc ttcctgtcca
     5161 tgggttgttc gaaagagacg ataaaactct ggtcgagcct catgttgtac acatcaccag
     5221 gcgggttttg ttccccatct ttcaaagcaa aattatagat gatcggcttg cgtgtgtcga
     5281 actgccagat gtaggattct cccaacgtgt tttcgagtgt gtcctgtagc ttcggatcaa
     5341 cccttacctg atagctggtt ccacttgtca gtaattcctc tggctgaaac acatacaccg
     5401 atgaattcac ccattcacct ttgccgttga tggcgggtgt gaatttcaac ggttggggga
     5461 gtttgtcctg ttcttcgacg atgttgagcg gcacaacggg gtgattgaaa atgaccgtga
     5521 tggtcgagtt caagtctact tcttgagtgt cgggtgcggg aaagacctgt gacacggaaa
     5581 gtgtctccac ggtcatgaat tcgattttga tctcttcttc tggggatgta ccgtcttttg
     5641 caatggctga ggtggtgatg accgccatgt attcagcggc gggtgtcaac ttggaatcag
     5701 gggagaagga aaatgtgcgg gtgtcgagcc atgtcaattg accgggaaca ggctccccgt
     5761 ttgagagaag cgagaatgct ttttctgtcg cggctttgtc catatcgcga tcgaatgtga
     5821 attggatgga gggttcaaga gtcaggcgtt gtccctcgat cggattttgc gcgataactt
     5881 gcggccccgt tggctcggct tcgatggaca ggggggcatt gttgaaaaag atgctttttg
     5941 ttagaacaaa tgccagtgca aaaatggcga ttacgccaat gatgcgcaaa gttttttgat
     6001 ttttcatggt gccgcctccc aaacgagtat gccttttgtg aagagagatg tcaagtgacc
     6061 ctttttaggt tttagggctt ttctctaa
//
LOCUS       DAOQJC010000182         5944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_7598_length_5944_cov_10.267108, whole genome shotgun sequence.
ACCESSION   DAOQJC010000182 DAOQJC010000000
VERSION     DAOQJC010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5944
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7598_length_5944_cov_10.267108"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PLE39_17925"
     CDS             complement(<1..965)
                     /locus_tag="PLE39_17925"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNE70268.1"
                     /translation="MTEVVIAGIGQTEVGEHWEISLRDLAYDAIHAAMQDAGGLRPQA
                     LFVGNMLAPNLSRQAHVGTLLADYAGLGGIEAVTVEAAGASGGAALRQGYLAIASGMV
                     DVALVVGVEKFTDKVGAEVDEALATIGDSDFEAVQGMTPTSQAALLMKRYMHENDVPA
                     DGFAGFALTAHANGAANKHAMFRKAIKPETYAKAEMVSDPINMFDMAPNADGAAALVL
                     TRRDLLPSNSKSQLVKIAGSAASSDTLALHDRKDILYFDTAQLSAGKAMKQAGIVLDQ
                     VNFFEYHDMFSIYAALQLEAVGFAIKGKGWKLAADGSIGLKGKIP"
     gene            complement(978..1964)
                     /locus_tag="PLE39_17930"
     CDS             complement(978..1964)
                     /locus_tag="PLE39_17930"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNE70269.1"
                     /translation="MAFFDLPLPELKNYRPTREEPADFDAFWKSTLAETRVHPLDAKF
                     EKVEYGLIAQETYDVTFNGFGGQPVKGWLILPAQGIRSQRTAPLPVVVEFIGYGGGRQ
                     FGFNWLFWASAGYAHFVMDTRGQGSTWSSGDTPDLYADGGNAHFPGSMTKGILEPQHY
                     YYRRVFTDAVRAIEAARSHPEVDEARVAVTGGSQGGGISIAAAGLVPEVAVAMPDVPF
                     LCHYRRATEISDAYPYKEIAEYCHVHRDKLETVFNTLSYFDGVNFAARAKAKTLFSTG
                     LMDQVCPPSTVYAAYNHWAGEKNIKVYTYNGHEGGQSFHDLEKLQFLKSIWK"
     gene            complement(2042..3118)
                     /locus_tag="PLE39_17935"
     CDS             complement(2042..3118)
                     /locus_tag="PLE39_17935"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HNE70270.1"
                     /translation="MTTPQPSPTLLKPVKPVGIVGYGAYVPRFRLPAKEVARVWTGKT
                     SGLPIKEKAVPGLDEDVITMSIEAARNAMARAQIDPTELRAVWVGSESHPYAVKPTST
                     LVAEAIGAVPNTQAADWEFACKAGTEALVAAMGLVGSGMGHYAMAIGMDTAQGKPGDA
                     LEYTAGAGGGAYIVGPAEESLAVINASYSYVTDTPDFWRREYQKYPEHGMRFTGEPAY
                     FKHITEAATHLMEASGTTAKDYKWAVFHQPNTKFPQRVAGQLGFSMEQIEPGLLVPII
                     GNTYAGAAMIGLTATLDIAQPGDRILVVSFGSGAGSDAFDITVTDKAPARQSLATKTR
                     EYIARRSEIDYATYVRYRGKLAMK"
     gene            complement(3223..4215)
                     /locus_tag="PLE39_17940"
     CDS             complement(3223..4215)
                     /locus_tag="PLE39_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70271.1"
                     /translation="MSNDNKNFIGIGIVLLACICGFMATVGIAAAWSQRFSKAPSVVY
                     SKITTFNIPDTIIEVRESCSDGQCIDACIQHVKNQGLESDLDLQNYHDEEVTLIYYNV
                     DENSIHDPRVPEVPENLLAFQQDKSTHEKIWNYFNVLIPREHQGNLVQFVVYTSQESS
                     GKHEDTITGNWILKYNILSAEDVIETTETLVYLYGYHLMLSPEQQIPWGEICHQEYLH
                     GCPTPESYANQFYLRFWKDIFAEWEEFDDNLQKREEDMGLFYTKYQDQFINEFAAEDP
                     VKDMVESWTEFMLYPRHTGDSIVDQKVNFFGEFPELVELREKVITGICTYEQTP"
     gene            complement(4522..5277)
                     /locus_tag="PLE39_17945"
     CDS             complement(4522..5277)
                     /locus_tag="PLE39_17945"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNE70272.1"
                     /translation="MRTVVLSDVHGNCLALETVMEDAKKKGFDQIVCNGDMIQSGPQP
                     HETVQLLRELKCPVVMGNSDAWLLTGIETDADSISEERRKKLDIVREWSLTKLNEEDR
                     AFINSFQPTITVELGGDKQLLAFHGSPISFDQVLSPYTPEDEFQEALKPYAGHILTGG
                     HTHLQFTRRLRDSQNFFFNPGSVGVAVNPEHTNEQNLLDPWAEYAILTVEKSNMRLEF
                     VRASLDMDKMTEIYRASDRPFADVMMDQYLSYK"
     gene            complement(5281..5763)
                     /locus_tag="PLE39_17950"
                     /pseudo
     CDS             complement(5281..5763)
                     /locus_tag="PLE39_17950"
                     /inference="COORDINATES: protein motif:HMM:NF015216.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkylmercury lyase family protein"
BASE COUNT         1337 a         1444 c         1568 g         1595 t
ORIGIN      
        1 caagggattt tccctttcaa tccgatcgaa ccatcggcgg ctaacttcca gcctttgcct
       61 ttgatggcga atccgactgc ttcgagctgc agggcggcgt aaatgctgaa catgtcgtgg
      121 tattcgaaga agttgacctg atcgagcaca atgcctgcct gtttcatggc tttccctgcg
      181 gagagttgag ccgtgtcaaa atacagaatg tcttttctgt cgtgcaatgc cagtgtgtct
      241 gatgaggcgg cggagccagc gatctttacg agctgactct ttgaattgga cgggagcagg
      301 tcacggcgag tcagaacgag cgcggcagcg ccatccgcat tgggggccat gtcgaacata
      361 ttgatcgggt cgctgaccat ttccgctttg gcgtaggttt cgggttttat ggctttgcgg
      421 aacatggcat gtttgttggc ggcgccgttg gcgtgtgcgg tgagtgcaaa gcctgcaaaa
      481 ccatctgcag ggacatcgtt ctcatgcatg tagcgtttca tcaaaagcgc ggcttgtgag
      541 gtgggggtca tgccctgcac ggcttcaaaa tcagaatcgc cgatggtggc gagggcctca
      601 tccacttctg cgccgacttt gtctgtaaat ttttccacac cgaccacgag ggctacgtcc
      661 accattccgc ttgcaatggc gagataaccc tggcggagtg ctgcaccgcc tgatgcgccc
      721 gccgcttcca ctgtgacggc ttcaatgccg cccaaccctg catagtcagc caacaatgtg
      781 ccgacatggg cctgccgcga aaggttcggc gcgagcatgt tgcccacgaa cagtgcttgt
      841 ggtctgaggc cgcccgcatc ttgcatggcg gcgtgaatgg catcgtaggc aagatcgcgc
      901 aagctgatct cccaatgttc gccgacttcg gtttgtccaa ttcctgcgat aacgacttct
      961 gtcatgaatc ctctagctta tttccaaatg cttttcagaa actgtaattt ctcaaggtcg
     1021 tgaaaacttt gtccgccttc atgtccgttg taggtataga ccttgatatt tttttcgcca
     1081 gcccaatggt tgtaggctgc atacacagtt gaaggtgggc agacctgatc catcaagcca
     1141 gtggaaaata aagttttggc tttggcccgt gcggcaaaat tcactccgtc aaaataggaa
     1201 agcgtgttga acacagtttc cagcttgtca cgatgaacat ggcaatattc ggcgatctct
     1261 ttgtagggat acgcatcgct gatctcggtg gcacggcggt aatgacaaag gaacggcaca
     1321 tcgggcatgg ctacggcgac ctcgggtaca agccctgccg cggcgatgga gattcctccg
     1381 ccttgagagc cgcctgtcac tgcgacacgc gcttcatcca cttcgggatg gctgcgtgct
     1441 gcttcgatgg cacgcacagc gtcggtgaag actctgcgat agtaatagtg ctgtggttca
     1501 aggatgccct tagtcatact gcctggaaaa tgggcattgc cgccatcggc atatagatca
     1561 ggggtgtcac ctgatgacca tgtgctgcct tgtccgcggg tatccatgac gaagtgagcg
     1621 taacctgcac ttgcccaaaa cagccaattg aatccaaatt gacgtccgcc gccgtagccg
     1681 atgaactcga caacgacggg taggggtgca gtacgctgag accttatgcc ttgtgcgggg
     1741 agaatcaacc agcctttgac gggttgtcca ccgaagccat tgaaggttac atcgtaggtt
     1801 tcctgcgcga taaggccgta ttccactttt tcaaattttg cgtcgagagg atgtacccgc
     1861 gtttcggcga gggtggattt ccaaaacgcg tcaaagtccg cgggttcttc acgcgtgggg
     1921 cggtagtttt ttagctcggg gaggggaaga tcgaagaagg ccatgtcagt atgtagtgaa
     1981 taggggatag tgaatagtgg gttttgcaaa tcccattcac tagctcctat tccctatcct
     2041 tttatttcat cgccagcttg ccgcgatacc gcacataggt ggcgtaatca atttcactgc
     2101 ggcgggcgat gtattcgcgg gtttttgtgg caagactctg gcgggcgggg gctttatcgg
     2161 tgacggtgat gtcaaatgcg tctgaccctg ccccagagcc gaaagagacg acgaggatcc
     2221 tgtcaccggg ttgggcgata tcgagagtcg cggtcaggcc gatcatggct gcgcctgcat
     2281 acgtgtttcc gatgatggga accaacagtc cgggctcgat ctgctccatt gaaaatccaa
     2341 gctgccccgc aacacgttgc gggaatttgg tgttcggctg gtggaagacc gcccatttgt
     2401 aatctttggc ggtggtgccg ctggcttcca taagatgggt ggcggcttcg gtgatgtgtt
     2461 tgaagtaggc aggttcgccc gtgaagcgca tgccgtgttc gggatatttc tgatattcac
     2521 gccgccaaaa atcgggtgta tcggtgacgt atgaatatga agcattgatg accgccaatg
     2581 actcttcggc tgggcctacg atatatgcgc cgccacctgc gcctgcggtg tattcaagcg
     2641 cgtcgccggg tttgccttgg gcggtgtcca tgccgatggc catggcgtag tgtcccatgc
     2701 ctgagccgac caaacccatc gcagcgacga gggcttcggt gcctgcttta caggcgaatt
     2761 cccaatccgc ggcctgggtg ttgggtacgg cgccgatcgc ttctgcgacc agcgttgaag
     2821 tgggtttcac cgcatacgga tgcgattcgg acccgaccca caccgcacgg agttcagtcg
     2881 ggtcaatttg cgcacgcgcc atcgcattcc gcgccgcttc gatggacatg gtgatcacgt
     2941 cttcatcgag cccgggtacg gctttctctt tgatggggag tccgctggtt ttacctgtcc
     3001 atacgcgtgc aacttccttc gcgggcaagc ggaagcgcgg aacataagcg ccatacccga
     3061 caatgccaac aggtttgacg ggtttgagga gggtggggga gggttgggga gttgtcattg
     3121 gataaccttt gaatagattg ctctcattct atgggtggaa ggcttgcaaa acctatcttg
     3181 ttggtccgga aaggttgcat attgaaagat aaaaccgttt ttttagggtg tctgttcgta
     3241 cgtgcaaatt ccagttatga ccttttcacg gagttcaacc aattcgggaa attcgccgaa
     3301 aaaattaact ttttgatcta cgatcgaatc gccggtatga cgtgggtata acataaattc
     3361 cgtccatgac tcaaccatgt cttttacagg atcttccgcg gcaaactcgt taataaactg
     3421 atcctggtat tttgtgtaaa acagtcccat atcttcttcg cgcttttgga ggttgtcatc
     3481 aaactcctcc cattcagcga aaatatcctt ccagaatctc aggtaaaact gattcgcata
     3541 tgactctggg gttgggcatc catgcagata ttcctgatgg caaatttctc cccacggaat
     3601 ttgttgttcc gggcttagca tgagatgata tccgtatagg taaaccaagg tctctgtcgt
     3661 ttcgattaca tcttctgcag aaaggatgtt gtatttgagt atccagtttc ctgttattgt
     3721 atcttcatgc ttgccgcttg attcctgact ggtgtagact acaaattgaa ctaggtttcc
     3781 ctgatgttca cgaggtatga gtacgttgaa ataattccaa atcttttcgt gcgtggattt
     3841 atcctgttga aaagctagca ggttttcagg tacttctggc acacgagggt cgtggatcga
     3901 attttcgtcg acgttgtaat aaatcaacgt aacctcttca tcgtggtagt tttgcaaatc
     3961 aagatcactt tccagacctt gattcttaac atgctgtata caggcatcta tacattgacc
     4021 atcggaacat gactccctga cttcaatgat tgtatctgga atattgaacg tggttatctt
     4081 tgaataaacc acagacgggg ctttcgaaaa cctttgggac caggcggctg caatgccaac
     4141 cgttgccata aacccgcaaa tacaagcgag tagaacgatt cctattccaa tgaaattttt
     4201 gttgtcgttt gacatattga catacctgtt ttagttcaga ttagtatccc ttgaaaaacc
     4261 ctctgctcag gttcgatatg aagccatatt tgcaatgtgt ggtcttttgg taattggcag
     4321 cgaattttga cttctaaccg cctgagggtg tttcatcata attgatgaaa cacgtatttt
     4381 ggataagtcc attacctttc aggatctcca aaatatcatc aacgattttg ctcatgttgc
     4441 aaatcctgac ttgaatatac caaaactttg acgtcaaccc tgccagcatt gaaatctctg
     4501 caaccttggc aggcttattt cctatttgta agataaatat tgatccatca ttacgtccgc
     4561 aaaaggcctg tcgcttgcgc gatagatttc agtcatctta tccatatcca atgaagcgcg
     4621 gacaaactcc agccgcatgt tggatttttc gaccgttaga atggcgtatt ctgcccaggg
     4681 atcgagtaga ttttgttcgt tcgtatgttc gggattgacg gcaacaccga cgctgccggg
     4741 gttgaaaaag aaattctggc tgtcgcgcaa tcggcgcgta aattgaagat gcgtgtgccc
     4801 accggtgagg atatgacctg catacggttt gagagcttct tgaaattcat cttctggtgt
     4861 gtaaggagaa aggacctggt cgaacgatat gggcgagccg tgaaaagcca gtagttgttt
     4921 atcgcctccc aattcaaccg tgatggtcgg ttgaaaggaa ttgataaaag cccggtcttc
     4981 ttcgtttagc ttggtcaatg accactcgcg gacaatatcc agtttcttgc gccgttcttc
     5041 cgatatcgaa tctgcatcgg tctcgatccc ggtcaatagc catgcatcgg aattacccat
     5101 cacgactggg catttcaact cgcgtaggag ttggacagtc tcatgtggct gcggtccgct
     5161 ttgaatcata tcgccattac agacaatctg gtcgaagcct ttctttttgg catcttccat
     5221 tacggtttcg agcgccaggc aattgccatg aacatcagat aaaactactg ttctcatttt
     5281 tcaactccgt ggataccaaa acttggaagt caacccagcc tgcctgaaaa cctctgcaac
     5341 ctgctcaatg ctccgcccat gaaaatccac ggataggcgg ttactgtacc agagtttcga
     5401 gagttcccat acttgatcga tcgtcaggat ttccccgcga gcgaggcggt ttcgcttgca
     5461 ccacttgtca atccattctt ccgaccggaa gagcagcatg gtggctcaag tgtagacgag
     5521 gtcgtcatac cagcgtgaaa aagaaagtgg aaagtggaca agtaattcgc tgtttgtcac
     5581 cttgccgtct ttgacttcca gttgaaccaa atcgttgctt tcggtgcaaa ccgcttcgat
     5641 gaccgcgtca cagtgtagcg cggcggggat tccgagcatg tcccaggcgc aattggcaaa
     5701 gtaggtcttc ccattggcat ggactttgaa gtcagtagga atgcccgaga agggatttgc
     5761 catgcggatg gtcagagtct ctggctcgag gaaaaaggaa tgacggttgt tcaattcctt
     5821 gtagatttcg ccagcttctt cagttttgat gttaaagtgc gcagcggtct cttccacgct
     5881 gggtgggtgg gttgtgtctg caatgtgggt gtaaacgaag tgtcgtacct gccagaggag
     5941 atct
//
LOCUS       DAOQJC010000183         5866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_7752_length_5866_cov_9.591809, whole genome shotgun sequence.
ACCESSION   DAOQJC010000183 DAOQJC010000000
VERSION     DAOQJC010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7752_length_5866_cov_9.591809"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PLE39_17955"
     CDS             complement(<1..888)
                     /locus_tag="PLE39_17955"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE70273.1"
                     /translation="MTKPTALIVEDDRDIVALFRHVLDIAGYQTEIMLNGKEAMSRLE
                     GFQPNIVLLDLQLPGMSGIEILSRMRADDRLRNVPVVVITAYAPYADSLPVEPDLLLM
                     KPVDIHQLSSLVQRLQATQGGLSESVHDRTTGLYTFPFFKVRLTFSLERIKQTEFRQF
                     GVLFADVAKINELKTKMPATEVNLFFRKLADHFKATLRPTDTMAWSLDNEFFLTLIED
                     IPTPEAPLRIAGRVRDSMKKFLETNDYGMNLRVSIGVLLCDYEYNDIEEILKDIDLAR
                     ARLREGLFTNPSVFDQSMLR"
     gene            1011..2345
                     /gene="yqeC"
                     /locus_tag="PLE39_17960"
     CDS             1011..2345
                     /gene="yqeC"
                     /locus_tag="PLE39_17960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HNE70274.1"
                     /translation="MDLAQALRLPLPASYHTVSLVGAGGKTTALFQLAQSLIQHGAPS
                     VILTNTTHLGDWQVAMAEHHVVAEDLPTLQDIPFSGITLVTGGFDHGRVMPVNESVLT
                     WLHENCRAKNIPLLIEADGSRQKSLKAPAAHEPPIPEFSDTVIVVAGLSVLGKPLTDE
                     HVHRAEVFSTLGELPLGQPVDPEALGRVLAHPQGGLKNIPPHARRVVLLNQADTPALR
                     SIGGNLAPTLLDAFDSVIVSALQKNSFQTFEQTAGIILAAGKATRFGAPKQILDWKGK
                     PFVRQVAETALHSGLWPVVVVTGAFSADVESALSNLPVKIIHNPNYTQGQSTSIHAGI
                     EVISRQAGAAIFLLADQPQIPTEVIRALVETHTRDLSPIIAPLVLEEKRANPVLFDRV
                     TFPSLLNITGDVGGRAIFSDYKVEYLPWHDDRLLLDVDKPEDYQRLVEDETL"
     gene            2358..2936
                     /locus_tag="PLE39_17965"
     CDS             2358..2936
                     /locus_tag="PLE39_17965"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNE70275.1"
                     /translation="MISAIVLAAGASKRMGEPKMLMPWGKSTVLQTIISTLQAASVND
                     ILVVTGGARQQVEMLVGKTVQTVFNSEYEKGEMLSSIQLGLSVKMHEASAALICLGDQ
                     PQVKERTVRSVCEAFLHNKARIVVPSYEMQRGHPWLIARPLWDEFLALKPPRTPRDFL
                     KKHAGKIQYVTVDTPSIIEDLDTPEDYLKYKP"
     gene            3014..3934
                     /locus_tag="PLE39_17970"
     CDS             3014..3934
                     /locus_tag="PLE39_17970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNE70276.1"
                     /translation="MKYFIIVNPVSGRGLGERSISQIESSLRENGLDFTLARTERMWH
                     AADLAEGAARDGYDVIVCASGDGTINEAINGIMKARKDGHTQAAFSALSIGTGNDFSG
                     GTGIPTNLNDSLKSLIANKRKKIDIGIVKGGDYPEGRYFGNGIGVGFDAAVGNEAIKV
                     RWTRGLPAYLIGVIKTVFLYYNPAQVEIVLDNQETIKQVSLMISVMNGRRMGGGFQMA
                     PESQPDDGFFDLCIAETASKGRILMLIPHFIRGTQMTQPEVKMRRAKKISIKSLDRTF
                     PAHADGEFICLSGSELTLELLPRELEIVCA"
     gene            3946..4626
                     /locus_tag="PLE39_17975"
     CDS             3946..4626
                     /locus_tag="PLE39_17975"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE70277.1"
                     /translation="MKFSLWLITFILRIYFRLTLRLDAQGMDTVPMQGPMIIISNHTG
                     QIEVPVLATLLQPRKITGWGKAEAFENPFLRWVFGAWGIIPVHRGEADMTALKSALRS
                     LKDGLIFGIAPEGTRNYSGKLRRALPGAVTLALHSGAPILPAAHWGGENFMKNFKQLK
                     RTDFHLRMGEPFVIDTKGAKVTAEMRQQIVDEMMVQLALLLPEEYRGEYTEMCKHETQ
                     FIKKQNAG"
     gene            complement(4646..5074)
                     /locus_tag="PLE39_17980"
     CDS             complement(4646..5074)
                     /locus_tag="PLE39_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE70278.1"
                     /translation="MRIDIVTKADEELYEAFQRLVPQLTNNNPPPSLNDLADLVRDSS
                     STLMVARNEKQEIVGALTLAVYRVPTGIRSIIEDVIVDTSARGQGIGEALMQRAIEIA
                     KEKGAGNISLTSNPLRESANRLYLRVGFKKRETNAYQMKF"
     gene            complement(5064..5792)
                     /locus_tag="PLE39_17985"
     CDS             complement(5064..5792)
                     /locus_tag="PLE39_17985"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNE70279.1"
                     /translation="MTTSAISENKPNPLRVIAAWGVHLFTATGAIWGFLAMLAIFEHD
                     WRSMIIYMVIAMLVDGFDGMLARWADVKTYANGIDGALLDNILDYLNYVVVPALFLME
                     AGVLPYGLQMIGAFSILITSAYQFTQTDAKTDDHHFKGFPSYWNVMALYMLLMNLPVW
                     VNFGFLVAFNILVFVPIKYIYPSRNSYLRTLTLALTYLYGAIGIWGLLLYPNTPKWVV
                     WASFIYVGYYLVLSLIPKNKSNAN"
BASE COUNT         1480 a         1564 c         1531 g         1291 t
ORIGIN      
        1 gcgcagcatg gattgatcga acaccgaagg gttggtaaac aaaccttcac gcagacgggc
       61 gcgggcaagg tctatgtctt tcaaaatctc ttcgatgtcg ttgtattcat aatcacacag
      121 cagcacaccg atgctcacgc gtaaattcat gccgtagtca ttggtttcga gaaatttctt
      181 catagagtca cggacgcgcc ccgcgatacg caacggcgct tcgggggtgg ggatatcttc
      241 gatcaaggtc aggaaaaact cattatccag cgaccaggcc atggtgtcgg ttgggcgaag
      301 cgtggctttg aaatgatccg ccagtttgcg aaagaaaaga ttcacttccg tggcgggcat
      361 ctttgttttc agttcgttga tcttcgccac atctgcaaac aaaacgccga attgtcgaaa
      421 ctcggtctgt ttgattcttt cgagtgaaaa ggtcaagcgg accttgaaaa acgggaaggt
      481 gtaaagcccc gtcgtcctgt catgcactga ttcgctcaaa ccgccctgcg tagcctgcaa
      541 acgctgaacc aggctgctca gttggtgaat gtccactggc ttcatcaaaa ggagatcagg
      601 ttccacgggc aggctgtcgg catacggcgc atacgctgtg atgaccacaa caggcacatt
      661 cctcaacctg tcatcggcgc gcatacgtga caagatctca ataccagaca tgcccggtaa
      721 ttgcagatcc agcaaaacga tattcggttg aaacccttca aggcggctca ttgcctcttt
      781 gccgttgagc atgatctcgg tttgatatcc tgcaatatct agcacatgcc gaaacagggc
      841 tacaatatcg cggtcgtcct caacgatgag cgcagttggt ttggtcaaag cctgctacct
      901 ttccgataaa aaaacaaaaa aatcacataa agataactca ttatacaatt gaagatgggg
      961 aaaacggaat aacaaaatgg attgcaatac ttctgattta caattgaagc atggatcttg
     1021 cacaagccct gcgcctgcct ttgcccgcat catatcacac cgtaagcctg gtcggtgcgg
     1081 gcggtaaaac caccgccctg ttccaactgg ctcaaagcct gattcagcat ggggctccat
     1141 cggtcattct gacgaacacc acacacctgg gagattggca ggtggcaatg gcagagcatc
     1201 acgtcgttgc ggaggacctg cccacgctac aagacattcc attctctggc attacccttg
     1261 taacaggcgg ttttgatcat ggcagagtca tgcctgtgaa cgaaagtgta ttgacttggc
     1321 tacacgaaaa ctgccgagca aaaaatattc ccttgcttat cgaggcagat gggtcccgcc
     1381 aaaaatccct gaaagcgcct gccgcccacg aaccgcccat cccagaattt tcggacacag
     1441 tcattgtcgt tgcagggctg agcgtgcttg gaaaaccact caccgatgaa catgtccacc
     1501 gtgctgaggt cttttcaact ctgggtgaat tgccacttgg gcaacccgtt gaccccgagg
     1561 cacttggccg tgtcctcgcc cacccacaag gcgggctgaa aaacattccg ccccatgcac
     1621 ggcgtgtggt tctgctcaat caggccgaca cacccgcgct gcgatccatc ggcggcaatc
     1681 tggcgcccac cctgcttgat gcgtttgatt ccgtcatcgt cagtgctttg cagaaaaaca
     1741 gttttcaaac attcgagcaa accgcaggca tcatcctcgc cgctggaaaa gcgacccgct
     1801 ttggtgcgcc aaaacaaata ttggattgga agggcaagcc gttcgtgcga caggtggcgg
     1861 aaactgcgct tcattctgga ctttggcccg ttgtggtcgt cactggcgct ttctccgctg
     1921 acgttgaatc tgccttaagc aaccttcccg taaagatcat ccacaacccc aactacaccc
     1981 agggacaaag cacctccatt catgcaggca ttgaagtcat cagcaggcag gctggagcag
     2041 ccatcttcct cctcgctgac caaccgcaga ttcccacaga agtgattcgc gccctggtcg
     2101 aaacacacac acgagattta tctcccatca ttgcgccgct ggtattggaa gaaaaacgcg
     2161 ccaaccccgt gttattcgac cgagtcacat ttcccagttt actcaacatc acaggcgatg
     2221 taggcggacg ggcgattttc tcagattaca aagtagaata cctgccatgg catgatgaca
     2281 ggttattgct tgatgtggat aaacctgaag actatcaaag attggttgaa gatgaaactt
     2341 tgtaaaagag gcaaaaaatg atcagcgcca ttgtactggc tgcaggcgca tcgaaacgca
     2401 tgggcgaacc caagatgctg atgccctggg gaaaaagcac ggtactacaa accatcatct
     2461 ccactttgca ggcggcaagc gtgaacgata ttctcgttgt cacaggcggg gccaggcaac
     2521 aggttgaaat gctggtcggg aaaaccgtcc aaaccgtatt caattcggaa tatgaaaagg
     2581 gcgaaatgct cagctcgatc caactggggt tgtcggtgaa aatgcatgag gcgagtgcag
     2641 cgctcatttg tctcggtgac cagccccagg tcaaggaaag aaccgtgcgc agcgtctgcg
     2701 aagcattcct ccacaacaag gccaggatcg tggtgccgag ttacgaaatg cagcgcggac
     2761 atccctggct gatagcccga ccattatggg atgaattcct tgcactgaag ccgccacgaa
     2821 ccccgcgtga ttttttgaaa aagcacgcag gcaagatcca atatgtaacc gtggatacgc
     2881 ccagcatcat cgaagacctc gacacccccg aagactattt gaaatataaa ccgtgagcgg
     2941 tgcgggcagt gcagatttga caatccgcct tacaaaagat acactcactt caatttccaa
     3001 ttgggagcgc agcgtgaaat atttcatcat tgttaatcca gtatcggggc ggggcctcgg
     3061 cgaaagatcc atttcccaga tcgaatccag cttgcgtgaa aacgggctgg attttacgtt
     3121 ggcgcgcacc gaacgcatgt ggcacgccgc cgatcttgct gaaggtgccg cacgtgacgg
     3181 atacgatgtg atcgtatgcg ccagcggcga tggcaccatt aatgaagcca tcaacggcat
     3241 catgaaagcc cgcaaagacg gtcacacgca ggcggcgttt tcagccctga gcattggcac
     3301 gggcaacgat ttttcgggcg gcacgggcat ccccaccaac ctcaacgaca gcctgaaatc
     3361 gctgatcgca aacaaacgca aaaaaattga catcggcatt gtcaaaggcg gcgattatcc
     3421 cgaaggccgt tatttcggca acggcatcgg cgttggtttc gatgcggctg tcggcaacga
     3481 agccatcaaa gtccgttgga cacgcggact gcctgcctac ctgattggcg tgatcaaaac
     3541 cgttttcctt tattacaatc ccgcgcaggt cgagatcgtt ctcgataatc aagaaaccat
     3601 caaacaggtt tcgctgatga tctctgtgat gaacggtcgg cgcatgggcg gcggttttca
     3661 aatggccccc gaaagccagc ccgatgacgg cttcttcgat ctgtgcatcg ccgaaaccgc
     3721 ttccaaaggg cgcattctca tgctcatccc ccacttcatc cgcgggaccc agatgaccca
     3781 gcccgaagta aaaatgcgac gcgccaaaaa gatctcgatc aaatcactgg atcggacttt
     3841 ccccgcacat gcggacgggg aatttatctg cctcagcggt tcagaattga cccttgaact
     3901 gctgccgcgt gaattggaaa tcgtctgcgc ttgagcattt tccccatgaa attttccctc
     3961 tggcttatca ctttcatcct gcggatctat ttccgcctca ccctgcgcct cgatgcgcaa
     4021 ggcatggaca ccgtccccat gcagggaccg atgatcatca tctcgaacca tacggggcag
     4081 atcgaagtgc ccgtcctcgc caccttgttg cagccgcgca aaatcacagg ctggggcaag
     4141 gccgaggcgt ttgagaatcc atttttgcgc tgggtcttcg gcgcgtgggg catcatcccc
     4201 gtccatcgcg gtgaagcgga tatgaccgca ctcaagtcgg cgctgcgctc tttgaaggat
     4261 ggtctcattt ttggcattgc ccccgaaggc acccgcaatt attcgggcaa attacgccgc
     4321 gccctgcctg gtgcagtgac tttggctctg cattctggcg cgccgatcct ccccgcggca
     4381 cattggggcg gagagaattt tatgaaaaac ttcaagcagt tgaagcggac agattttcat
     4441 ttacggatgg gagagccgtt cgtgatcgac acaaagggcg ccaaggtgac ggctgaaatg
     4501 cgccaacaga tcgtggacga aatgatggtg cagcttgcgc ttttgctgcc cgaagaatac
     4561 cgcggcgaat acacagagat gtgcaagcac gaaacacaat ttatcaaaaa acaaaacgcg
     4621 ggctgaaaag tccgcgtttt gttttttaga atttcatttg ataggcgttg gtctcgcgct
     4681 ttttgaatcc cacccgcaaa tacaatcgat tcgccgactc gcgcagtgga tttgacgtga
     4741 gcgagatgtt gcccgcacct ttttctttgg cgatctcgat ggcacgctgc atcagcgctt
     4801 cgccgatgcc ctgtccacgg gcggaggtgt caacgatgac atcttcgatg atggagcgga
     4861 tgcctgtcgg cacacggtaa acggccaacg tgagcgcgcc gacgatctcc tgcttttcgt
     4921 tgcgagccac catcagcgtg gacgatgaat ctcggacgag gtcagccaga tcgttaagag
     4981 aaggaggcgg attgttattc gtcaactgcg gcacgaggcg ttgaaacgct tcgtagagtt
     5041 cctcatccgc ctttgtaacg atgtcaattc gcattggatt tgtttttggg gatcaggctc
     5101 aacaccagat aatatccgac ataaatgaac gaagcccaga ccacccactt gggggtgttg
     5161 gggtaaagca gcaagcccca gatgccaatg gcgccgtaga gataagtaag ggcgagcgta
     5221 agggtccgca ggtaactgtt gcggctggga taaatatatt tgatgggaac aaagacaagg
     5281 atattgaacg cgaccaaaaa gccaaagttc acccacacag gcaggttcat caacagcatg
     5341 tacaacgcca tcacgttcca atacgaggga aagcccttaa agtgatggtc gtcggtcttg
     5401 gcatcggttt gggtgaactg atacgccgag gtgataagaa tggaaaacgc gccgatcatt
     5461 tgcaagccgt agggcagcac acccgcttcc atgagaaaga gcgcgggcac aaccacgtaa
     5521 ttcaggtaat cgagaatgtt atcaagcaag gcgccatcaa tgccgttggc gtatgtcttg
     5581 acatcagccc agcgtgccaa catgccgtca aaaccatcca ccagcatcgc gatgaccatg
     5641 tagatgatca tggagcgcca gtcatgctcg aaaatggcaa gcatggccag aaagccccag
     5701 atcgcgcctg tggctgtgaa aagatgcact ccccatgcgg cgatgacgcg gagagggttg
     5761 ggtttgtttt ctgaaattgc ggaggttgtc atgggttctg tccttggttg gggtgaaagg
     5821 tgctggaatt atactcatgt ctgggggaag ttgaggggtg caggtt
//
LOCUS       DAOQJC010000184         5860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_7765_length_5860_cov_10.278208, whole genome shotgun sequence.
ACCESSION   DAOQJC010000184 DAOQJC010000000
VERSION     DAOQJC010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7765_length_5860_cov_10.278208"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLE39_17990"
     CDS             <1..243
                     /locus_tag="PLE39_17990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70280.1"
                     /translation="LAAIRAMQLTFTDMQTWATANGVTLGSGSGQPGSGMNLSAEERA
                     TKQAAEGMTGQTPGSGGGGGAALLDAVINYLEGVAQ"
     gene            complement(790..1395)
                     /locus_tag="PLE39_17995"
     CDS             complement(790..1395)
                     /locus_tag="PLE39_17995"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE70281.1"
                     /translation="MPRPKKTDDEIQFMREQILDTALVILQSDGAEAITSRAIAERLN
                     VAHMSLFTYFKNQAEILDMLREREMAKWAAKQIGFEQRAQLEDVKQVVRELLEFYVSF
                     ARENPDLFRLAWVIPQATGESLEEERQRRKATVENLSRILHLGMEQGAFIKRDPFLAA
                     STVLAMINVPNILFHSGKLVDINLRDQMVKEILFAAMKYLE"
     gene            1482..1934
                     /locus_tag="PLE39_18000"
     CDS             1482..1934
                     /locus_tag="PLE39_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70282.1"
                     /translation="MFEKLFPKTVDNQYRGMTVARWIFVAMTIVIVGRSLAHIFLPDG
                     GAQSIATIPLDAFPPIAASVIIGMFAYWGLSQLLLGLLYVLVLWRYQSLIPLMWFSLF
                     VEWTGRFLLGRFYKPIETVGTAPGAVGNHFVPVLAIIMLALSLRERKS"
     gene            1972..2988
                     /locus_tag="PLE39_18005"
     CDS             1972..2988
                     /locus_tag="PLE39_18005"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE70283.1"
                     /translation="MSNFMRWVLMILAGIALLLALFVWAYAPLRARNLAAEYEQFRAE
                     HQAEHDSVDAQFNQKDTVVNGIQWHYVDEGNPDGTVILFLHGLPEGWYSWSKVLPLVD
                     QEYRLIAIDMKGYGRSDLIDTDYNWHTVASQILDLMTSLGIDRFYVVSHDWGTIIGSV
                     LVGDHPEHILGYVRMQADLIQGESRDRPSFSVYLQKPQWLFFQNNWIASYLYQDAGWL
                     IDTVYSSRMLTNLSQADRDYLVYEYSRPGVAEMNPRYFNPANWDLTSAVGKICKNNFP
                     FPVLQLQADSDPSQPPSIFANVNTKCPNVQMEWVTNANHFDNFDQPQQVADAINNFIH
                     VNSK"
     gene            3012..3182
                     /locus_tag="PLE39_18010"
     CDS             3012..3182
                     /locus_tag="PLE39_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70284.1"
                     /translation="MWFMLSLEVLLRKLVGLMKPVTFAHTPPGAISNYVMLPLALALW
                     SAVKHTRSNENQ"
     gene            3193..3624
                     /locus_tag="PLE39_18015"
     CDS             3193..3624
                     /locus_tag="PLE39_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70285.1"
                     /translation="MKEKMKDIYWLPANILLFIGGLDVLRGFMHTFLLTWSATNFAKF
                     DMATVPQDQVFLLGVFGISNFLTGFIYFLVSRKARELSPYVLMIIPLTYILGLLGIWS
                     GNVHGQAAFDGKYFMLAYFSVCIITFIIFQFQKRGQANNAA"
     gene            3632..4807
                     /locus_tag="PLE39_18020"
     CDS             3632..4807
                     /locus_tag="PLE39_18020"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNE70286.1"
                     /translation="MKKIASLLLLGLLAVLLGGSVGAVAAGFLHFIEWAQHLLWEVIS
                     VNLPWQALFVCTLGGLLVGLCQRFLGDHPKGIQEAVVEIRQTGRLDYVHLPQGMTTAS
                     VSLIFGASLGPESAIMDLLGGLSTWVGDVLRSIRENLNQAQVSESGSRLVRLTNAWPN
                     GLAFLVGALAFGRSLNGLYSGGLLHFTYVFQWMDLLWSIPLGLLGAGAGLLYTFLQKQ
                     TERWVEPLKDHPIRRSLLGGFVLGLAALFLPQVLFSGQHALQPAYDQALQLGFWNLLF
                     IGLARLLLTALLLSTGWKGGQFLPIMFAAATLGLAMSVPFPAVPAPVAVLAAMAALIA
                     VVLPKPLIALVLMGLLFPLNTLGISLVGVGVVVFGKRLWLRTVQPAQQSRPASAASD"
     gene            4826..5464
                     /locus_tag="PLE39_18025"
     CDS             4826..5464
                     /locus_tag="PLE39_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70287.1"
                     /translation="MQFAQLLSWVVLALLYLAFWMWYGGQGKPLSQAETKTLLAEMRR
                     RAGKQAGDDAPLLEHFSNLAKSDDGHEFYMVNLLKFRQKAAYPPGSVFGDDPFAANQR
                     YNKAIVPLLLKYGGHPVFLGQVQGRFIHPADVDEWDQVGIVRYRSRRDMFRMAMEMID
                     NGADIHKWAALEKTQVFPVKPLINIAYVRAMVALLLFGFGIFVYFTASTLFH"
BASE COUNT         1368 a         1423 c         1464 g         1605 t
ORIGIN      
        1 ctggcagcga tccgtgccat gcaattgact ttcaccgata tgcagacctg ggcaaccgcc
       61 aatggcgtta cccttggttc tggtagcgga caacctggct ctggcatgaa tctttccgcc
      121 gaagaacgcg caaccaagca agccgctgaa ggaatgactg gtcaaacccc gggcagcggt
      181 ggcggtggcg gagcagcgct tttggatgca gtaatcaatt atctggaagg tgtagcccaa
      241 taattcttct ttctaacact aaactaaagc accccagatg aacttacttt tgcgaagcag
      301 gggtgcttta gttttttaac ccatttctgc tttaactttt tgcgaatttc atcaaaggag
      361 ttatctttac tttcatactt acattacgat ccaaatcagc atgtaaataa atatttatgt
      421 ggatgcaaat acagcaattt ctaacatggt aaacttgatt tgtttcccca aaacttgaag
      481 cctgcaatat tagcacaagg gaactaccaa ttttttgcaa aatcacccta aagaccaccc
      541 attttcaacg agaattttcc atgaaaccct cttcagccac agcccaagcg aatccaaata
      601 tcgctttggc gatgtaatct caagagaaca caatatttgc tagcggtgaa gttggttatc
      661 cccacatata gtggttccac cactttgtca gcttatcaaa acgcttatgc ctccgttgcg
      721 atgggggcgt aagcgtttta gcctcaataa gacagtgaag atttttgctg catcaaggag
      781 tcgtagctct tattccaaat atttcattgc tgcaaaaaga atctctttta ccatctgatc
      841 acgcaaatta atatcaacca gttttccact gtgaaagaga atgttcggca catttatcat
      901 tgctaaaact gtgcttgccg caaggaatgg atcacgtttt ataaacgctc cctgttccat
      961 acccaaatga agtattctgg acagattttc aacagttgct tttcgccgtt gacgttcttc
     1021 ttctaagctc tcaccggtgg cttgcggtat aacccaggcc aggcgaaaca gatcggggtt
     1081 ttcgcgagca aacgaaacat agaattccag taattcgcgc accacctgct tcacatcctc
     1141 taattgggct cgctgttcaa atccgatctg ttttgccgcc cacttggcca tctcgcgttc
     1201 ccgcagcata tccagaattt ctgcctggtt tttgaagtaa gtgaataagg acatgtgggc
     1261 tacattcaac ctttcggcaa ttgcacgtga tgtaatggcc tcagcgccat ctgactgaag
     1321 aatgacaagc gcagtgtcga gaatttgctc gcgcatgaac tgaatttcat catctgtttt
     1381 ttttgggcgg ggcatacaaa attccttaga tagggttgat tatccttgac atatttatta
     1441 taccacgtat aatttatgca acgtataaaa aaggagatcc catgtttgaa aaactatttc
     1501 ccaagaccgt tgacaaccag tatcgcggca tgactgttgc caggtggata tttgtagcga
     1561 tgacgatcgt gattgttggg cgctcgcttg ctcatatctt ccttcccgac ggtggcgcgc
     1621 agtccatcgc caccatccca ttagatgcgt tcccgccaat tgcagcatcg gtgatcattg
     1681 gcatgttcgc ttactggggc ttgtcgcaat tgctgctcgg tttgttatac gtgcttgtac
     1741 tatggcgtta ccagtccttg attccgttga tgtggttttc ccttttcgtt gagtggaccg
     1801 ggcgtttctt gctgggtagg ttttacaaac ccatcgaaac tgtaggtact gctcctggcg
     1861 cggtcggtaa tcatttcgtg ccagtgttgg ccataattat gctggccctt tccttgcggg
     1921 aacggaaaag ttgaacgaat tcacccggaa ggtaaaaagg agtattttgt gatgagcaat
     1981 tttatgagat gggttttgat gatcctggcc gggattgccc tgctgctggc gcttttcgtt
     2041 tgggcctatg cacccctgcg cgcccgaaac ctggccgccg agtatgaaca gttccgcgca
     2101 gaacaccagg cggagcatga ttccgttgat gcccaattca accaaaagga tacagtggtg
     2161 aacggcatcc aatggcacta cgtggacgaa ggcaatccag atgggacggt catactgttt
     2221 ctgcacggac tgcctgaagg ttggtattcg tggagcaagg tactgcccct agtggatcaa
     2281 gagtaccgcc tgattgctat tgatatgaaa ggttatggcc gctccgacct gattgacacg
     2341 gattacaact ggcataccgt ggcatcccag atccttgacc tgatgaccag tctgggtatt
     2401 gatcggttct atgtggtctc tcatgattgg gggacaatca ttggcagcgt actggtcggc
     2461 gaccacccgg aacacattct cggttatgta cgcatgcaag ctgacttaat ccagggcgaa
     2521 agccgggata ggccatcgtt ttcggtttat ctacaaaaac ctcagtggct tttcttccag
     2581 aacaactgga ttgcttccta tctgtaccag gatgccggtt ggctgattga tacggtttat
     2641 tccagccgca tgttgaccaa tctcagccag gctgaccgcg attacctggt gtacgaatat
     2701 tcccgccctg gtgtagctga gatgaacccc agatatttca accccgccaa ttgggatctg
     2761 acctccgcgg ttggaaaaat ctgcaaaaac aactttccct ttccggtgtt gcagctccag
     2821 gccgatagtg acccgtctca gccgccttcc atttttgcca atgtgaacac aaaatgcccc
     2881 aacgttcaga tggaatgggt gacaaatgcc aaccactttg ataatttcga ccaaccgcaa
     2941 caggtggccg atgccatcaa caattttatc catgtgaata gcaaataggc aatcgctcgt
     3001 tggtgccttt catgtggttc atgctgagcc tggaagtgct gctgcgcaaa ctggtaggac
     3061 tcatgaagcc ggtcaccttt gcccacacac cgcccggcgc gattagcaac tatgtgatgc
     3121 tcccattggc gctggcgctg tggagcgcgg tgaagcacac tagatcgaac gagaatcagt
     3181 gaggtaaaca aaatgaaaga aaaaatgaag gatatttact ggctaccggc caacatcttg
     3241 ctctttatcg gcgggttgga tgtgctgcgc ggctttatgc acaccttcct gctaacctgg
     3301 tcggctacca atttcgcaaa attcgatatg gcgaccgtac cacaggatca ggtatttttg
     3361 ctcggcgtat ttggtatctc gaacttcctg acggggttca tttattttct cgttagccgc
     3421 aaggcccggg aactcagtcc atatgtattg atgatcatcc ccctgactta cattttgggc
     3481 ctgctcggaa tttggtctgg caacgtgcat gggcaggctg cctttgatgg aaagtatttc
     3541 atgttagcct attttagcgt ttgcatcatt acctttatta tttttcaatt tcaaaaaaga
     3601 ggccaggcga acaacgccgc ttaggataag aatgaagaaa atcgcatcac ttttactgtt
     3661 gggtcttctt gccgttctgc tgggtggatc ggtgggcgcc gtcgcggcgg gcttcctgca
     3721 cttcatcgaa tgggcacagc acttattgtg ggaggtaatc tcagtcaacc tgccctggca
     3781 agcgctgttt gtgtgcacac tgggtggttt attggtcggt ctttgccagc gtttccttgg
     3841 ggatcatccc aagggtattc aagaagcggt ggttgagatt cgtcagactg ggcggttgga
     3901 ttatgttcac ctgccacagg gaatgaccac agcttctgtc tcattgattt tcggcgccag
     3961 cctggggccc gaatctgcca ttatggattt gctcggcggg ctaagcacct gggtgggtga
     4021 tgtgctccgg tcaatccgtg aaaacttgaa tcaggcgcaa gtctctgagt caggcagtcg
     4081 cctggtcagg ctgaccaatg cctggcccaa cgggctggcg tttctggttg gggctctcgc
     4141 ttttgggcgg tcattgaacg ggttgtacag cggcggcctg ttgcatttta cttatgtctt
     4201 ccaatggatg gacctgcttt ggagtattcc actgggcttg cttggagccg gcgcagggtt
     4261 gctatatacc ttcttgcaga agcagacaga gcgctgggtg gaaccactca aggatcaccc
     4321 catccggcgc agtctgttgg gtggttttgt cctgggtttg gcagctctgt tcctgccgca
     4381 ggtgctgttt tccggccaac atgccctcca gccggcctat gaccaggccc ttcaactggg
     4441 tttctggaac ctgctattca tcggtctggc gcgcctactg ctcacagccc tgctactctc
     4501 cacgggttgg aaaggcggac aatttctgcc catcatgttt gctgcggcca cattggggtt
     4561 ggccatgagt gtacccttcc cggccgtgcc agctccggta gcggttctgg cggcgatggc
     4621 agctttgatc gctgtagtac tgccaaaacc gctgattgct ctggtgctta tgggcctgct
     4681 ttttccacta aatacgctgg gcatctcttt agttggggtt ggagtggttg tttttgggaa
     4741 gcgcctctgg ctgcggacag ttcaacctgc ccaacaatcc cggcctgcgt cagccgccag
     4801 cgattgatga aacggataaa aaaacatgca atttgctcaa ctgctatcct gggttgtttt
     4861 ggcgctgttg tatctggctt tttggatgtg gtatggcggc caaggcaagc cgctctctca
     4921 ggctgaaacc aagactttgc tggccgagat gcgccggcgc gccggtaaac aggcgggaga
     4981 cgatgcgcct ttactggaac actttagcaa cctggccaaa agcgatgatg gacacgagtt
     5041 ttatatggtg aacctgctca agtttcgtca gaaagcagcc tatcctccag gcagtgtttt
     5101 cggagatgat ccatttgcgg caaaccaacg ctacaataaa gccattgtgc cactcttact
     5161 gaaatacggt gggcatccgg tctttcttgg ccaggtacag gggcgattta tacacccggc
     5221 tgatgtggat gaatgggatc aggtgggcat tgtgcgctat cgcagccgca gggatatgtt
     5281 caggatggca atggaaatga ttgacaacgg cgccgacatc cacaaatggg ctgcactgga
     5341 gaaaacccag gtttttccgg tgaaaccgct cattaatatt gcctacgttc gcgccatggt
     5401 cgctctccta ctcttcggat ttgggatatt tgtatatttc actgccagca ccctattcca
     5461 ttagtaaata aaggatggtt gccctaaaac atcgcctaag gtatcatgca agcgcaagag
     5521 acaacgccaa aagaaaggaa aactgcacaa aacagtggcg tttttgccgt aaaaatcaca
     5581 gttttgtgta caaaatgcca tgaaaatcat gtctgctacc actcaaagtt cgccaaatat
     5641 tgcattggcg atgtaatctc aagggaacac agtatttggt atccgcaagg cttgttttgc
     5701 ctaaataggg tggtcggaaa gcctcaaata gtgaaaatga agacgccttt gcgaatgcag
     5761 aggcgtcttg cttggctatt attgactagt ttctaatatt tgtatgaggg ttggacaagt
     5821 caaatgactt gttacgtttt atcgtaacca gagcgtaatc
//
LOCUS       DAOQJC010000185         5830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_7835_length_5830_cov_9.644848, whole genome shotgun sequence.
ACCESSION   DAOQJC010000185 DAOQJC010000000
VERSION     DAOQJC010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7835_length_5830_cov_9.644848"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1666)
                     /locus_tag="PLE39_18030"
     CDS             complement(173..1666)
                     /locus_tag="PLE39_18030"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE70288.1"
                     /translation="MSSRILLLNPARHFIANEAGVGYLTPLGLVLIGGPLMDAGFEVK
                     LIDHDMNGWPMERLLGEVAAYQPNYILLGHSGSTASHNMVVQTVWELRKAFPEIRIIY
                     GGVYPSYAFESILRQVPEIDVIVRGEGEKTIVDLISAWEKGVNLEQVAGIAWRRNGEI
                     VVNRPQPAIQNLDDYRPGWELLDWKYYQMFGFDHAAGLQFSRGCTLTCSYCGQWMFWK
                     KWRHRSPQNIVENMKLLKEKFGVRLIWFADENFAADRDLAKDLLERIIAADLGLSLNL
                     NMTAADVVRDADLLPLYKKAGVDYIVMGVESLKDSVVAGIRKNNPFATSKEAVRLLRQ
                     HDIISLTNIIYGLEDESWQTLFEKFRGMLELDSDILNAMYLTPHFWTSDGKATDPAKI
                     IQADLAKWSYRNQVIATSYLNPFELFLGVKLTEALFHLRPKALWRLVAHRDGRYRQIM
                     RTSILAGIRVVLAEIWEFAFDTKFVKQGKLTEIIGAERSRQDATPLA"
     gene            1785..2672
                     /locus_tag="PLE39_18035"
     CDS             1785..2672
                     /locus_tag="PLE39_18035"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE70289.1"
                     /translation="MQKTKLIPYLEALFAVVVWGASFIATKIAVGQMSPIAVVWLRFA
                     MGIPILFAAVVMRKQFAFPKGMEWLYFALLGFLGISFHQWLQSNGLQTAQATTTAWIV
                     STSPAFIAILGWLILKEKLNLTQSLGIVLAMVGVLAVVSKGDLATIAIGKFGTYGDFL
                     ILISSVNWAVFSILSRRGLKDHPSTRMTFWVMTIGWLITTTAFLAGQNYTEISRLDSR
                     GWMAMIFLGIFTTGLAYIAWFDALSHLPAAQTGAFLFVEPLTSMVVAALILSEEITLV
                     SILGGAVILVGIWLVNRKG"
     gene            2704..3441
                     /locus_tag="PLE39_18040"
     CDS             2704..3441
                     /locus_tag="PLE39_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70290.1"
                     /translation="MNARATQNANVISTEKGWRLGIQKGDSLSYRDAQLDDYSLLPRR
                     KFPHRALTLSLRAKTTGSSLPGTWGFGLWNDPFGLSLGFGGSPFRLPALPNAVWFFYG
                     SQENYLSFSPDTRKTAANGFMAQTFRSPKFHPRLIPAGMALPFSRKATRNLLGKVIDE
                     DSVALSVDVTQWHGYRLEWSAKRSAFWVDDVLVFESPVSPNPRLGVILWIDNQFAAFT
                     PEGKIGFGVLENPEPAWLEIEDLKIVS"
     gene            complement(3452..4486)
                     /locus_tag="PLE39_18045"
     CDS             complement(3452..4486)
                     /locus_tag="PLE39_18045"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNE70291.1"
                     /translation="MHINFAMWRKASWQLIKMDDKKEWDALDVVSKWLIATRSAVTLV
                     TVYSCVIAGILAARDGHFSWVPFLIVTLGLFIAHGTNNILNDYTDFNRGVDRDNYFRT
                     QYGVHPLVQKFWDNSTSIRWFIVSGVLATLSGVYALFYTKFSMTTIGLFAFGAVVLLA
                     YTYPFKYWGLGEFAIFLIWGPIMIAGVYFVLSGIWSWNVVLAGIPFGLSVASINVAKH
                     IDKHDPDKEKGVGTFPVRVGEANARRVDQLAIVLIYAVIVYLVFVPRYFTPAMLIVLL
                     AYKEALMVIGVLNKPKPAQAPEGWPAWPVWFSGFAFQHNRKFGGLLIMGLIIDALLRA
                     FLPTFWPGLF"
     gene            complement(4728..5753)
                     /locus_tag="PLE39_18050"
     CDS             complement(4728..5753)
                     /locus_tag="PLE39_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNE70292.1"
                     /translation="MNFAMWKKALNVIPEVSKEEWDQLDVISRWLISTRAAVLIMTFI
                     SAALAGLFAWRDGSFSFIPWIALVFGLIMAHASNNIFNDYTDFVRGVDKDNYYRTMYG
                     AQPIASGLMTQRQHLTYFAVTGFLALVAGLYLIFSNANDPIIWMLLAAGAFFVLFYTW
                     PLKGLGLGEVAVLVVWGPLMIGGGYYVLAHEWNWSVAIASLPYVLGVTTVIFGKHIDK
                     ISVDKAKNIHTLPVVIGEKAARYAVIAMMVLPYIFTGYLIATKFFTPVMLIVLLAIPS
                     LRESLPPLSKPKPDTRPEGFPDGQGGWPLYFAPIAFRNNRSFGSLFMLGLLVDVALRI
                     FLPAFWR"
BASE COUNT         1496 a         1501 c         1517 g         1316 t
ORIGIN      
        1 atttccataa aagagcctcc aaagtgcaat tatacccgat gggtacgctc gccgagggca
       61 ctggccggtc accattacgc ccatcgacgc tatacacgct ccagggaatt ggatatggag
      121 ccgccgcgaa tccctttacc agaaactgct ggaaaatttc aagaactgat cttcaagcca
      181 gcggagtggc atcctgcctt gagcgttccg cgccgattat ctctgttagc ttcccctgtt
      241 ttacaaattt cgtatcgaag gcaaattccc aaatttccgc caacaccaca cggatgcccg
      301 ccaggatcga agttcgcatg atctggcgat agcgtccatc tcggtgtgca accaaccgcc
      361 acaacgcctt cgggcggaga tggaacaaag cctcggtcag tttcacccca agaaacaatt
      421 caaatggatt caagtacgaa gtggcaatga cctgatttcg atacgaccat tttgcaaggt
      481 cggcttgaat gatcttcgct ggatccgtgg ctttcccatc cgatgtccaa aagtgcgggg
      541 tcaggtacat ggcgttcaaa atatcggaat ccaactcaag catgcccctg aatttctcaa
      601 agagggtctg ccacgattca tcttcaagac catagatgat gtttgtcagg ctgatgatat
      661 cgtgctggcg caataaccgc accgcttctt ttgaagtcgc gaagggattg ttcttgcgga
      721 tgccagccac aacactatcc ttgagcgact ccacgcccat cacaatgtag tccacacctg
      781 cctttttata aagcggaagc aggtccgcat cgcggacaac gtcagcggcg gtcatgttca
      841 agttgagcga gaggccaagg tcagcggcga tgatgcgttc aagcagatcc tttgctaggt
      901 cacggtcggc tgcaaaattc tcatcggcaa accagatcag cctcacaccg aatttttcct
      961 tgagcagttt catgttctcc acaatattct gaggactgcg atgccgccac ttcttccaaa
     1021 acatccactg accgcaataa ctgcaggtga gtgtacaacc gcggctaaat tgcaggcccg
     1081 cggcatggtc aaagccgaac atttgatagt atttccaatc cagcagttcc cagccagggc
     1141 ggtaatcgtc caggttttga atcgcaggct gcggacgatt gaccacgatc tcaccatttc
     1201 tgcgccaggc aattcccgca acctgttcca ggtttacgcc tttttcccaa gctgaaataa
     1261 ggtcaacgat tgttttctcc ccctcaccgc ggacaatgac atcgatctct ggaacctgcc
     1321 gaagaatgct ctcaaaggca taggatggat aaacaccccc ataaatgatt cgaatctctg
     1381 ggaaggcctt gcgaagctcc cacacggtct gcacaaccat gttgtgcgaa gctgtggaac
     1441 ctgaatgtcc aagcaaaata tagtttggct ggtatgcagc cacctccccc aacaaccgct
     1501 ccatgggcca gccgttcatg tcgtggtcga tcagttttac ttcaaaacca gcatccatca
     1561 atggcccgcc gatcaatacc aaccccaatg gcgtcaggta cccaacccca gcctcatttg
     1621 caatgaaatg acgtgcagga tttaacaaga gaattcgtga tgacatattg cctccataaa
     1681 tcagataaaa aagaccataa acatctttct ttttgtgaat tttatcacca gcaaaactcg
     1741 tgtcaagaga tttataatca gccaaatttc ctagaggaac cctcatgcaa aaaacaaaac
     1801 ttatccccta tcttgaagcc ctgttcgctg ttgttgtttg gggcgcgtct ttcattgcca
     1861 caaaaatcgc cgtcgggcag atgtctccca ttgctgtagt gtggctgcga tttgcgatgg
     1921 gcatcccgat cctgtttgcc gcagtggtta tgcgcaaaca gttcgccttt cccaaaggaa
     1981 tggaatggct ctattttgcc ctgctcggct ttttaggaat ctcatttcat caatggctgc
     2041 aatcaaacgg actgcaaaca gcccaagcca ccaccaccgc atggatcgtt tccacatcgc
     2101 cggcattcat cgccattctc ggctggctga tcttgaagga aaaactcaac ctgacacaat
     2161 cactgggcat tgtgctggcc atggttggtg tgctggcagt ggtaagcaaa ggagaccttg
     2221 ccaccattgc catcggcaaa ttcggcacct atggtgattt tctcatcctc atcagctcag
     2281 tcaattgggc agtgttctcc attttgtcac gccgcggtct gaaagaccac ccctccacac
     2341 ggatgacatt ttgggtaatg accatcggct ggctgatcac cactacggca tttttagcgg
     2401 gccaaaacta caccgaaatt tcccgcctcg attcgcgcgg ctggatggca atgatctttc
     2461 tcggcatctt caccacaggg ctggcctaca ttgcctggtt cgatgcgctc agccacctgc
     2521 ccgctgcaca aacaggcgcg ttcctttttg ttgagccgct caccagcatg gtggtggctg
     2581 cactcatttt gagcgaagag atcacgctcg tatccatttt aggcggcgcg gtgattctgg
     2641 tgggcatttg gctggtaaat agaaaaggat gaaaagataa ggatgaatta tgaaggatga
     2701 aggatgaatg caagggcaac tcagaatgca aacgtaatct caactgaaaa ggggtggcgg
     2761 ctgggaatac agaaagggga ttctcttagc tatcgagacg cccaactcga tgactattcc
     2821 ctgctgccga gaaggaagtt tccgcatcgc gctctaactt tgagtttgcg tgcaaaaact
     2881 acaggcagtt ccctccccgg aacatggggc tttgggctgt ggaacgaccc gtttgggttg
     2941 tcactggggt ttgggggaag tccgttccga ttgcccgcat tgccgaatgc ggtttggttc
     3001 ttttatgggt cgcaggaaaa ttatttatcg tttagccctg atacccgaaa aacagcggca
     3061 aacggattca tggcgcagac ctttcgctcg ccgaaatttc atccgcggtt gatccctgcg
     3121 gggatggcgc tgccgttctc gcgcaaggcg actcgtaatt tattaggaaa agtgattgat
     3181 gaggattcgg tcgctctcag cgtggatgtc actcaatggc atggatacag gctcgagtgg
     3241 agcgcgaagc ggtctgcttt ttgggtggat gatgttctcg tgtttgagtc gcccgtgagt
     3301 ccgaatccgc ggctgggggt catcctctgg atcgacaatc aatttgcggc attcacccct
     3361 gaaggaaaaa tcgggttcgg agttttggaa aaccccgaac ccgcatggtt ggaaattgaa
     3421 gatctaaaga tcgtttcgtg aatatttctg tttagaacag cccgggccag aaggtgggca
     3481 ggaaggcgcg caacagcgcg tcaatgatga ggcccatgat gagcaggccg ccgaatttgc
     3541 ggttgtgctg gaaggcaaaa ccagagaacc acaccggcca ggcaggccag ccttcaggag
     3601 cctgggcggg cttgggcttg ttcagtacgc cgatgaccat gagggcttcc ttgtatgcaa
     3661 ggagaacaat gagcatggca ggggtgaaat agcgcggaac aaacacaagg tagacgataa
     3721 cagcatagat caacacaatg gcaagctgat ccacacggcg ggcgttggcc tcccccacac
     3781 ggacggggaa tgtgccgacg cctttttcct tgtcggggtc atgcttgtca atgtgcttgg
     3841 caacgttgat gctggcaacg ctcaacccga acggaatgcc cgcaaggacg acgttccaac
     3901 tccagatgcc tgaaagcaca aagtacacac ccgcgatcat gatcggtccc cagatgagga
     3961 agatggcgaa ttcgccgaga ccccaatatt tgaagggata tgtgtaggcc agcagaacga
     4021 ccgcgccaaa ggcgaacagt ccgatggtgg tcattgagaa tttggtatag aacagggcgt
     4081 agacacctga aagggtggcc aacacaccgc tgacgataaa ccagcggatg gaggtggagt
     4141 tatcccagaa tttttgaacg aggggatgga caccgtactg ggtgcggaag tagttatccc
     4201 gatcaacgcc gcgattgaag tcagtgtaat cgttgaggat gttgttggtg ccgtgggcaa
     4261 tgaacaggcc aagggtgacg atgaggaacg gaacccatga gaagtgtcca tcgcgggcgg
     4321 cgaggatacc tgcaataacg caggaataaa cggtgacaag ggtcacagcg gaacgggtgg
     4381 cgatcaacca tttggaaacg acatcaaggg catcccactc tttcttgtcg tccatcttga
     4441 tgagttgcca cgaggcttta cgccacatag cgaaattaat gtgcatagta ctctctcctt
     4501 gagtaatatt ggagcccaat gcaagtgagc accacttcat caggcttatg aatgattcaa
     4561 aacaaccgtg cgagattata caaccgtttt gtgaaaatga ttactataaa atcccctggt
     4621 attgttctga attcacgccg ccgaggacgg cggctggagc ggaaaaactt ggagtcaaaa
     4681 agtccccgaa agatttctct ttcagggact ttgatcaggt caagctgtta gcgccagaag
     4741 gcggggagga agattctcag agctacatcc acgagcaggc cgagcatgaa gagcgagccg
     4801 aaggagcggt tgttgcggaa ggcgatgggt gcgaagtaga gcggccaacc tccctgcccg
     4861 tcgggaaaac cctcaggacg ggtatcaggc ttgggcttgg aaagaggcgg aaggctttcg
     4921 cgcagagacg gaatcgccag caggacgatc aacatcacag gcgtgaaaaa tttggtggcg
     4981 atcaaatatc cagtgaaaat atatggcagc accatcatcg caatgaccgc atagcgggcg
     5041 gctttttcgc cgatgaccac aggcagtgtg tggatgttct tggctttatc cacgctgatc
     5101 ttgtcgatgt gcttgccgaa gatgacggtg gtcacaccca gcacgtaggg caggctggca
     5161 atggcaaccg accaattcca ctcatgtgcc aacacataat acccgccgcc gatcatcagc
     5221 ggaccccaaa ccacgagcac agccacttcg ccaaggccga gtcccttgag cggccaggtg
     5281 taaaacaaca cgaagaacgc gccagccgca aggagcatcc aaataatggg gtcattggca
     5341 tttgagaaaa taagatacaa gcccgccacc aacgcgagga atcccgtcac agcaaagtag
     5401 gtcaggtgct gacgctgggt catcaacccc gaagcgatgg gttgggcgcc atacatggtg
     5461 cggtaatagt tgtccttgtc cacgccgcgc acgaagtcgg tgtaatcgtt gaagatgttg
     5521 ttactggcat gagccatgat gagaccaaag acaagcgcga tccacgggat aaagctgaac
     5581 gagccgtcgc gccaggcaaa aaggcccgcc agggccgccg aaataaaggt cataatgaga
     5641 acggctgcgc gggttgagat caaccatctc gagatcacat caagctgatc ccattcttct
     5701 ttagacacct caggaatcac attgagggcc ttcttccaca ttgcaaagtt catttgatac
     5761 tccttgaaaa ttttgacaat tataaccaat tcggtcttga atccatcttt ggaattggcg
     5821 gtttttttac
//
LOCUS       DAOQJC010000186         5825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_7844_length_5825_cov_9.871577, whole genome shotgun sequence.
ACCESSION   DAOQJC010000186 DAOQJC010000000
VERSION     DAOQJC010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7844_length_5825_cov_9.871577"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLE39_18055"
     CDS             <1..314
                     /locus_tag="PLE39_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70293.1"
                     /translation="LSAQGATATEAMSMLVQLMGALYFAFALMNWTAKDGVIGGIYAR
                     PVSLGNFAHFFAGALLLVKAQFSSEFSWLGFVVMLVYVFYALCFYWLVFRATGIKPPA
                     A"
     gene            complement(315..1127)
                     /locus_tag="PLE39_18060"
     CDS             complement(315..1127)
                     /locus_tag="PLE39_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70294.1"
                     /translation="MKNDAILPLTRMITAVVVFFLVTAFGILFFLPDRTGELFAWAIK
                     PHMSSMYFGSAYLGGAWILAQTAFGKRWHRVQAVFPAVTVFTTAMLIATLLHWDRFSL
                     GTIPFMAWLILYIISPFLIPALWIYNKRTDSNLPEESDLTVSPTARMITRIFGTLVLV
                     FVLIGFISPNLIIPIWPWTLTPLTARVLCGWLSVIGTGAWAISLDPRWTGWRAILEGI
                     FIAYSLIFIAAFMNPADFSTGIFNWYTAFMGIMLIAILVFYTSMESKRRKAS"
     assembly_gap    1186..1285
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1461..1847
                     /locus_tag="PLE39_18065"
     CDS             1461..1847
                     /locus_tag="PLE39_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70295.1"
                     /translation="MKKLIGLWIDRKRALILTLEEKKEQLKQIQSNLAAHTQFEGDTH
                     AKYFKAEDQMDRHLGEHMKKYYREIITSVRDAAAILIMGPGEAKFEFEKRLAHEHVKA
                     RVAMVETADKLTERQFTARVKKYFKS"
     gene            1996..2490
                     /locus_tag="PLE39_18070"
     CDS             1996..2490
                     /locus_tag="PLE39_18070"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNE70296.1"
                     /translation="MNTPKRYNPFLVTLHWLTVVLMLGAGLLSESGGDSPINFHMMLG
                     GALLVVTLIRIGVRFATKHPAQADTGNPLFNQLGQWMHYGLYLALFFILGMGALIANK
                     RNLLAVAMGTGSVTGRAGFLGDIHHLGWFLAILLILGHVGAALYHQFILKDNLLGRMW
                     FGKQ"
     gene            complement(2596..3375)
                     /locus_tag="PLE39_18075"
     CDS             complement(2596..3375)
                     /locus_tag="PLE39_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70297.1"
                     /translation="MNILMLKLILAPVIIGSASMAGRRWGAAVSGWLVGLPLTSGPVA
                     FFLALSHDRAFVFDSVRGTLSGGFSLVAFSLTYAWVSRKFNWVVSILSGVVVFASMTT
                     LLQNITIPFLPLFGAVIVVIFIGLRLMPKGQAVPPVSNPGGWDIPARILIGTSFILLI
                     TGIAPYIGSRLTGLLTTIPLYVSILTIFSHRHQGHAGAVNVLHGLILGLFSFAGFYLV
                     LGLLIERSSLAASFGSAILATLVVQGLSLLILRQRHIHKGE"
     gene            complement(3372..3989)
                     /locus_tag="PLE39_18080"
     CDS             complement(3372..3989)
                     /locus_tag="PLE39_18080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNE70298.1"
                     /translation="MNQFKIFAPIFPYLAVGVGLFFFNNAWAALFGFHIAILLTVLVL
                     RPSLSVRTLFKSRSPKWILASVLFCSTSGIGLYFLWPVFGVIGDLSAQLLAMGLTPVS
                     WPWFLAYFSLVNPFVEEYFWRGVLGNDSLKPYFGDVVFAGYHAMILWGRVHLVFILFA
                     VSVLVSAGWLWRQISREDGGLLSASLGHMAADFTILVTIYWMAVP"
     gene            complement(4018..4941)
                     /gene="mvk"
                     /locus_tag="PLE39_18085"
     CDS             complement(4018..4941)
                     /gene="mvk"
                     /locus_tag="PLE39_18085"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HNE70299.1"
                     /translation="MKASAHGKIILFGEHAAVFGHPALAVPVTQVHVDVEVLDSPRTG
                     IWIHAPIIDLHAELNSLPPDHAIASVILKVIQHFGISPLPNLEVNISSTIPVAAGLGS
                     GAAVSVALIRALSAFLSQPFSDEEVNSLAYEIEKIHHGTPSGIDNTVITYGKPVYFMK
                     DKPVEIFKVGRPFTIVIGDTGIAAPTKESVGDVRRLWIRDQFTVENIFNEIAQIALIG
                     RRSIESGRTEMLGELMDHNHEYLQQLTVSSPELDSLVEAARKAGALGAKMSGGGRGGN
                     MIALVDPARAESVAAALISAGAKRTVITEIK"
     gene            complement(4938..5810)
                     /locus_tag="PLE39_18090"
     CDS             complement(4938..5810)
                     /locus_tag="PLE39_18090"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNE70300.1"
                     /translation="MLTALIKTMRPRQWVTKNIFIFAALVADKQLFKPEAFLRTLAGF
                     ALFSLISSCVYIINDLADVEADRQHPEKKNRPIASGKLPVGVAVTAAVLFVAITFVLA
                     YLLTPSLLAVIGGYFVINLAYSKWLKHVPILDVLIISTGFVLRVGAGVTLIEVERFSP
                     WLYVVMTLLSLFLGFGKRRAELALLANGAGSHRKVLDGYTLPLLDQYIMIVSGATIVA
                     YSLYTFSAPNLPENHTMMLTIPFVVYAIFRYMYLIEVKHAGGAPEEVLLSDRPFQLAM
                     VLWGLTVLAIFYLS"
BASE COUNT         1575 a         1423 c         1532 g         1195 t
ORIGIN      
        1 ttttatcggc gcagggagcg acagccacag aggcaatgtc catgctggtg cagttgatgg
       61 gcgcattgta tttcgccttt gcgctgatga attggacggc caaagatggt gtgatcggcg
      121 gaatttacgc gcggcctgtg tcgctgggaa attttgctca tttctttgca ggggcgctgc
      181 tgttggtgaa ggcacaattc tccagcgaat tcagttggct gggatttgtt gtgatgcttg
      241 tgtatgtgtt ctatgcgctg tgtttttact ggcttgtgtt tcgagcaaca gggatcaaac
      301 cgccagccgc ctagttaact ggctttccgt ctcttgcttt ccatgctggt gtagaaaaca
      361 aggatcgcga tcaacatgat gcccatgaag gcggtgtacc agttaaagat gcctgtgctg
      421 aaatcggcgg ggttcatgaa tgcagcgatg aagatcaggc tgtaggcaat gaagatccct
      481 tcaaggatgg cgcgccatcc cgtccagcgc gggtcgaggg agatcgccca tgcaccggtg
      541 ccgatcacag aaagccatcc gcacagcaca cgcgcagtaa gcggagtcaa cgtccacggc
      601 cagatgggga tgatcagatt cggggagata aagccaatta gaacaaagac cagcacaagg
      661 gttccaaaga tgcgggtgat cattcgggca gttggtgaaa cggtcaggtc cgattcctca
      721 ggcaggttcg aatcggtccg cttgttgtat atccacagtg cggggatcaa gaacggggaa
      781 atgatgtaaa ggatcagcca ggccatgaaa gggatcgtgc caagcgaaaa gcggtcccaa
      841 tgcagcaggg tggcgatgag catggctgtg gtgaagacag tgaccgcagg gaagacggcc
      901 tgtacacgat gccagcgctt gccgaaggcg gtttgggcga ggatccatgc accgccgagg
      961 taggccgagc caaaatacat cgaagacatg tgcggtttga tggcccaggc aaacaattca
     1021 ccggtgcggt ctggcaggaa gaacaggatg ccgaaagccg ttaccaaaaa aaatacgaca
     1081 acggctgtga tcatacgggt aagggggaga atcgcgtcgt tcttcatcag gtcatccttt
     1141 caattttcaa agagcgggtt tgcgcaaaac tcagcctgtc cacgannnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnttcat ctctggcaaa tggttttgcg agaaccctaa
     1321 acggataagg tcaggcgata ttaccatgcc tcacacagcc gcacaatcgt ccatgccagc
     1381 ctttggattt gctttgcccc tcatagatat gtatactgag attgggtcgt gttcgctttt
     1441 caatgtcata aggaggttcc atgaaaaagc tcattggttt atggatcgac cgaaagaggg
     1501 cattgatctt aaccctcgaa gaaaaaaagg agcagctcaa acaaatccaa tcgaatcttg
     1561 cagcccatac ccagtttgaa ggggatactc acgcaaagta tttcaaagcg gaagatcaga
     1621 tggacaggca cttgggggaa cacatgaaaa aatattacag ggagatcatc accagtgtcc
     1681 gcgatgccgc tgccattttg atcatgggcc cgggcgaggc caagtttgaa ttcgagaaac
     1741 gcctggccca tgagcacgtc aaagccaggg tggcgatggt cgaaaccgca gacaaattga
     1801 ccgaacgaca atttacagcc agggtgaaga aatatttcaa aagctaggtt tcaaaaaaga
     1861 ttcccaatga caaattggga atcttttttt acagacgggg ttgtgtggaa attatgtgga
     1921 aatctcgaaa acaacttgat tgtcattttg agttaataaa gccaatataa aatctcgtga
     1981 aaaggagaga agaacatgaa cacacccaaa cgatataatc catttcttgt cacactgcac
     2041 tggcttaccg tggtgctgat gctcggggcg gggctgcttt ctgaaagcgg aggcgattct
     2101 ccgatcaatt ttcatatgat gttggggggc gccttgctgg ttgtgacgct cattcgcatt
     2161 ggggtgcgct tcgccaccaa acaccccgct caggcagata cgggcaatcc gctctttaat
     2221 cagcttggtc aatggatgca ttacggtcta tatcttgcgc tatttttcat ccttggcatg
     2281 ggcgccttga tcgcaaacaa gaggaatttg ctggctgtgg caatgggcac cgggtcggtc
     2341 acaggcaggg ctggttttct cggtgatatt caccatctcg gctggttcct tgccattttg
     2401 ctgatccttg gtcacgtggg tgcagcactg taccatcaat tcattctcaa agacaacctg
     2461 ctgggcagga tgtggtttgg aaagcaataa agaaacagat gcaatttcat caaaggatac
     2521 aaaaaacgcg gacacttctc aagtccgcgt tttttgtgtc agtcagcgct tgcacgatca
     2581 aaaatactga aaggtctatt cccctttgtg aatatgccgt tgccgcaaga tcagcaggga
     2641 taatccctgc acgaccaggg tggcgagaat cgcagatcca aatgaagctg caaggctcga
     2701 ccgctcgatc aacaagccaa gcacaagata aaaccctgca aacgaaaaca agccaaggat
     2761 caatccgtgc agcacattca ccgcgcctgc atggccttga tgccgatgtg aaaagatcgt
     2821 caaaatgctg acgtacaatg ggatcgtggt cagcagtccc gtcaggcgtg agccgatgta
     2881 gggggcaatg cctgtgatga gcaaaataaa actcgtgccg atcaaaatcc tcgcggggat
     2941 atcccagccg cctgggttgg aaacgggcgg cacggcttgt ccctttggca tcagccgcaa
     3001 gccgataaaa ataacgacga tcaccgcacc aaaaagcgga agaaacggga tcgtgatatt
     3061 ttgcagcaat gtggtcatgc ttgcaaacac gaccacaccc gacaaaatgg aaacgaccca
     3121 attaaacttc cgcgaaaccc aggcgtaggt gaggctgaat gcaacgaggg aaaagccgcc
     3181 gctcaacgta ccgcggactg aatcgaatac aaaggcacgg tcgtggctga gggcgaggaa
     3241 aaaagccacc ggccccgagg taagcggaag ccccaccagc catccgctca cggccgcccc
     3301 ccaccgacgc ccagccatac ttgccgagcc aatgatgacc ggggcgagga tcaatttcaa
     3361 catcaaaata ttcatgggac tgccatccaa tagatcgtta ccagaattgt aaaatcggcg
     3421 gccatgtgcc ccaatgaagc agaaagcaaa ccgccatctt cacgggagat ctgccgccat
     3481 aaccagcccg ccgaaaccag aacggaaact gcaaacaaaa taaagaccag atgcaccctg
     3541 ccccacagga tcatcgcatg ataacccgca aaaacgacat cgccgaaata cggtttgagc
     3601 gagtcattgc ccaacacacc gcgccagaaa tattcttcaa cgaatggatt gaccagtgaa
     3661 aaataagcaa ggaaccaggg ccatgatacg ggtgtcaatc ccatcgccaa aagttgcgcg
     3721 gagagatctc caatcacgcc aaacacaggc cagagaaaat acagtccaat tccgctggtg
     3781 ctgcaaaaaa ggacactggc tagaatccat tttggcgagc ggcttttgaa aagcgtgcgt
     3841 acagacaggc tgggacgcaa aaccaaaact gtcaacaaga tcgcgatgtg aaacccgaac
     3901 aaggctgccc atgcattgtt gaaaaaaaac aaccccactc ccacggcaag gtagggaaaa
     3961 atgggggcaa atattttgaa ttgattcata ttgaaaaaag tcaaccccgt tgtgtcgtta
     4021 tttgatctcg gtgataaccg ttcgctttgc acctgcagat attaaagccg ccgctacaga
     4081 ctcggccctg gcagggtcaa ccaacgcaat catattgccc ccgcgtccgc cgccgctcat
     4141 tttggcgccg agtgccccag ccttgcgcgc cgcctccacc aaagaatcca attcaggaga
     4201 agagactgtc agctgttgta gatattcatg gttgtgatcc atcagctcgc cgagcatttc
     4261 ggttcgtccg ctttcgatgg aacgcctgcc gatcaatgcg atctgcgcga tctcgttgaa
     4321 aatgttttcc accgtaaact ggtcgcggat ccacagtcgg cgcacatcgc ccaccgattc
     4381 ttttgtgggc gcggcaatgc ctgtatcacc gatgacgatg gtgaacggcc tgccaacctt
     4441 gaagatctcc acaggcttgt ctttcataaa ataaacgggt ttgccgtaag tgatgacggt
     4501 gttatcaatg cctgagggtg tgccgtgatg gatcttttcg atctcatagg caagtgaatt
     4561 cacttcttca tctgaaaatg gctgggagag gaaagccgaa agagcacgga tcaacgcaac
     4621 cgacaccgcc gccccagacc ccagccccgc cgcaacaggg atggtggatg agatattcac
     4681 ttcgagattc ggcagcgggg aaatgccaaa gtgctgaatc actttcagga tcaccgaagc
     4741 aattgcatgg tcaggcggaa gcgaattcaa ttcggcgtgc aggtcaatga tcggggcgtg
     4801 aatccaaatc cctgtgcggg gcgaatccaa aacctcgaca tccacatgaa cctgagtgac
     4861 gggcaccgcc agtgcaggat gtccaaacac cgctgcatgt tcgccgaaaa gaatgatctt
     4921 gccgtgtgcg gaggctttca tgaaaggtaa aagatcgcca atacggtcag tccccaaaga
     4981 accatcgcaa gttgaaatgg acggtctgaa agcagcactt cttcgggtgc gccgcctgca
     5041 tgtttcactt caatgagata catgtagcgg aagatggcat acaccacaaa gggaatggtc
     5101 agcatcatcg tgtgattctc gggcaggttg ggcgcggaga aagtgtacag cgagtaagcc
     5161 acaatggtgg cccccgaaac gatcatgatg tactggtcga gcaaaggcag ggtgtaacca
     5221 tcgagcactt tgcggtgtga gcccgctcca tttgcaagca acgcaagttc ggcgcggcgt
     5281 ttgccaaaac cgaggaagag cgaaagcaga gtcatcacca cgtaaagcca gggcgaaaat
     5341 ctttccactt caatgagggt aacacctgca cccacacgca gcacaaagcc cgttgaaata
     5401 atcaacacgt cgagaatcgg aacgtgcttg agccacttcg agtaagcaag gttgatgacg
     5461 aaatatccgc cgatcaccgc gagcaaagac ggcgtgagca ggtatgccag cacaaaagtg
     5521 atggccacaa acagaacagc ggcagtgacc gccacgccca caggtagttt acccgaggca
     5581 atggggcggt tcttcttttc agggtgctgg cggtcggctt ccacatcggc gagatcgttg
     5641 atgatgtaca cacaggaaga gatgaggctg aaaagcgcaa aacctgccag cgtacgcagg
     5701 aaggcttcgg gcttgaacaa ttgtttatct gcaacaagtg cggcgaagat gaaaatattt
     5761 tttgtcaccc actgccgcgg gcgcatggtt tttatgagag cggttaacat gaggatattt
     5821 tacct
//
LOCUS       DAOQJC010000187         5813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_7875_length_5813_cov_9.812088, whole genome shotgun sequence.
ACCESSION   DAOQJC010000187 DAOQJC010000000
VERSION     DAOQJC010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7875_length_5813_cov_9.812088"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..674
                     /locus_tag="PLE39_18095"
     CDS             129..674
                     /locus_tag="PLE39_18095"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNE70301.1"
                     /translation="MTDKKTHHIGTLSPEMALLGLLYKEPGHGYDLHRKVNTDLGQVW
                     HLSQSQAYAILKRLEMQGDISTKKISQGKLPSRQLLQMTAQGRQHFLNWLHTASGGST
                     RVIRMEFITRLYFLKMYLPQKITKAFEQQRTEINTHIKRLEENLHTLPTDQIYNRMSL
                     EMRVKQLVLTLEWLDENQNNF"
     gene            810..1619
                     /gene="modA"
                     /locus_tag="PLE39_18100"
     CDS             810..1619
                     /gene="modA"
                     /locus_tag="PLE39_18100"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE70302.1"
                     /translation="MKQIFVLTVSLSILAAACNTSIAQISTPTARETIPQTLIVFAAA
                     SLTEAFTEIGAAFDAANPGVNTTFNFAGSQILRTQIEEGAPVDIFASASSKEMESLLT
                     SNLVDSKAPRFFLTNKLVVILPENNPAALEKLEDLANPGIKIILATEEVPVGKYARLS
                     LNLMDASFGSGFKDKVLDNIVSNEDNVRQVVSKVQLGEADAGIVYVSDTMATSELKSI
                     EIPADLNVVAKYPIAPLIKSEHTDLATKFIEYVFSAEGQAILIKWGFTPVK"
     gene            1595..2452
                     /locus_tag="PLE39_18105"
     CDS             1595..2452
                     /locus_tag="PLE39_18105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE70303.1"
                     /translation="MGIYPSQMRFGLHMNKLQKFINYDFRDEKHSKWIFIIPSVVLLA
                     FFALPLAAIILRSLNLNFFENALSQQAFKALRLSLLTSSITTLCTAVFGTPLAYILAR
                     WKFTHKTWLELILELPIVLPPSVAGVALLITFGRNGLLGPFFKFFGISLPFTTTAVVL
                     AQLFVAAPLFLRSARIGFAHIDQQLEESARVEGANQWQLFSEVTLPLSARALASGAIL
                     TWTRALGEFGATILFAGNLEGMTQTMPMAIYLGFERNIGVALALSVVLIFVSVILLML
                     TKRLEKNKE"
     gene            complement(2563..3213)
                     /locus_tag="PLE39_18110"
     CDS             complement(2563..3213)
                     /locus_tag="PLE39_18110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70304.1"
                     /translation="MKDDVRKYIYGAVTVFLLGVLAWVAIVYVNSCGFTLTCIRGAQP
                     VVRTPIPTLLPATMPAAQTGDGKVVVSDKCRVAAVDLIGAWVEAGSSETEAFQFTDIN
                     GLNCESSFAEVMPLFVESNFWVSGSLSCVSCHSTDVTISLAQLDLSSYAGITSGSRRA
                     DAESKGTDILGGGKWEGSLLYDFLTTAKADVPGHTEAVSDLVIFAGTPAPLATPTP"
     gene            complement(3226..3783)
                     /locus_tag="PLE39_18115"
     CDS             complement(3226..3783)
                     /locus_tag="PLE39_18115"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNE70305.1"
                     /translation="MRLFKQLVVVFAVLAVLVGALMVFSYDVIKIDWIVFMEIQPSYS
                     AQENPLPVPAQSIPVEGAAYIPNLGAPSNPVPADETSIARGAELYTIHCLMCHGATGQ
                     GNGTISAFLVKKKPADLTSELVQSKTDGSLFLTISNGIFNPNNTLFPDTEFSGQMPPL
                     SENLSVRERWDLVNYIRTLTVPAAQ"
     gene            complement(3780..4316)
                     /locus_tag="PLE39_18120"
     CDS             complement(3780..4316)
                     /locus_tag="PLE39_18120"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNE70306.1"
                     /translation="MSESKIVDLLAVFPDLEPAANAIEQLRTLGVHDDCMNVISGVPV
                     TEAMLGRPHQWTNVPRIAMGGAILGFSGGFFLAFISPYIYPYPIQVSTQAFVPGPPSV
                     VVLFELTMLGMLLSTFLGVFLDSFFPNYRPMKYVPEISDGKIAILVECPHADEKKITD
                     ALNKMGAESVKPAEAQHL"
     gene            complement(4326..5753)
                     /locus_tag="PLE39_18125"
     CDS             complement(4326..5753)
                     /locus_tag="PLE39_18125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70307.1"
                     /translation="MSDTKHHEAHSHEEHLREKHLMLDPLPRGKMNDMVMESMLEKPT
                     WKYWVIFAFLSFVVVYFLFYKWGVLIAKGQGQAGVMRPDYWGIFLVNTVFWIGISHAG
                     TFISAILRVFRAEFRRPFTRAAELMTTFGLVQAGFSIFMHMGRVWLFYWLMPYPNQRL
                     LWPNLHSPLTWDLLAITTYLLSSTMYLFLPLIPDVAMARDRSTGWRKNLYKVLALGFR
                     GTEGEWTHLRNAMNIFAWAIIPVMFSVHTIVSWDFAAATRPGWSSTIFGPYFIVGALH
                     SGMGAAAVVLAVIRGTMKNMKYFVRGEHFDAIGKLMLIISMAWAYFFFNDYMVQWYGG
                     DKWTQQLLHFHEAGPLGWMWFGMLFCNIVVPWAILWNAKWRATPWAITIVGLVINVGM
                     WFERYIIVPISVTINRMPFTWRMYTPGIEVPMGIGTVALFILLYMAASKLIPLIPVWE
                     VQEGQMAHELKKFGRETVVAVSELE"
BASE COUNT         1598 a         1403 c         1435 g         1377 t
ORIGIN      
        1 agaaaaacgt attaaatata aagtcttaaa atgcaaacaa tctccaacca tggcaggaat
       61 ttatttcaaa aagttactcc tgtttttacg atatactcat caaatcaggc tacttgtttg
      121 gtgagtatat gacagacaaa aaaacacatc acatcggaac actatcacct gaaatggctt
      181 tacttggcct gctatacaag gaacccggtc atggctacga cctgcatcgt aaggtcaata
      241 cagaccttgg gcaggtctgg cacttaagtc aaagtcaggc atacgccatt ttaaaacggt
      301 tggaaatgca aggcgatatt tcgaccaaaa aaatctcaca gggaaaactc cccagccgtc
      361 agctgttgca aatgactgca caagggcgac aacatttctt aaactggctt catactgcca
      421 gtggcggaag cacccgtgtc atccgcatgg aatttattac ccgcttgtat tttctaaaaa
      481 tgtatctccc acaaaaaatt acaaaagctt tcgagcagca acgcactgaa atcaatacac
      541 acattaaacg tctggaagaa aatttgcaca cattgccaac cgaccaaatc tataaccgta
      601 tgagtttgga gatgcgtgta aagcaattgg ttctcacact ggaatggctg gacgagaatc
      661 agaacaactt ctaggttccc atgaaataaa cggggaataa ttcataaaac cgatctcatg
      721 tttcagtaaa agtatttgta tcgttcgaag attctacgct atactcactc agtgagtata
      781 aaactgtttt cccgatttcg gagagtttga tgaaacaaat attcgtccta actgtttctt
      841 tgtccatctt ggctgcggcc tgtaatacct ctatcgcaca aatatctact cccactgcca
      901 gggagacaat tccacaaact ttgatcgttt ttgctgcggc ctccttaact gaggcattta
      961 ccgagatcgg cgctgcgttt gatgccgcta atcctggcgt caacacaaca ttcaactttg
     1021 caggctcgca aatcctgcga actcaaatcg aagaaggtgc acctgttgat atatttgctt
     1081 ctgccagcag caaagaaatg gaatcccttc tcacaagtaa tcttgtcgat tcaaaggcgc
     1141 cacgtttttt tctgacaaat aaattagttg tcatcctgcc agaaaacaac cctgctgccc
     1201 tcgaaaaatt ggaagacctc gcaaacccag gcatcaaaat tattttagct acagaagaag
     1261 tacctgtcgg taagtacgca cgtctatccc tcaacttgat ggatgcttca tttggatcag
     1321 gtttcaagga caaagttttg gacaatatcg tttccaacga agacaatgtc aggcaggtgg
     1381 tatccaaagt ccagcttggc gaagcagatg caggtattgt ctatgtctct gacaccatgg
     1441 caacttccga attgaaatcc attgaaattc ccgctgactt gaatgtcgtc gcaaagtatc
     1501 ccattgcgcc attgattaaa tcagaacata cagatttagc tacaaaattc atcgagtatg
     1561 tattttcagc agaagggcag gcgatcctca tcaagtgggg atttacccca gtcaaatgag
     1621 attcggtctt catatgaata agttacaaaa atttattaat tacgatttcc gggatgaaaa
     1681 acactcaaaa tggatattca tcatccccag tgtggttttg cttgcttttt ttgcacttcc
     1741 gctggctgcg atcattctac gttcgctcaa tctgaatttc tttgaaaatg ccttatcgca
     1801 acaagctttc aaggcgctgc ggctaagcct gctaaccagt tcaatcacca ccttatgtac
     1861 agcagttttc ggcacacccc tcgcctatat tctggcacgt tggaaattta cacacaaaac
     1921 gtggctggag ttaattcttg agctccccat tgtgctccct ccctcggttg caggagtggc
     1981 gttattgatc actttcggac gaaacggatt actcggtccg ttttttaagt tttttgggat
     2041 cagcctgcca tttacaacaa ctgctgtggt gctggcacaa ttgttcgtgg cggcaccatt
     2101 attcctccgt tcagcacgaa tcggttttgc acacattgac caacagcttg aagaatctgc
     2161 gcgagtggaa ggagcaaatc aatggcaact attttcagag gtcacgctac cattatctgc
     2221 tcgagcgctt gcaagtgggg ccatcctgac atggacacgc gctctgggtg aattcggggc
     2281 aacaattcta ttcgcaggga atttggaggg tatgacccag accatgccaa tggccatcta
     2341 tcttggtttt gagcggaaca tcggtgtagc attggcactc tcagttgtgt tgatctttgt
     2401 atcggtcata ttgctcatgc ttactaaaag gttggaaaaa aacaaggaat aaaacttagc
     2461 aaaaaattca tataagtttt gcaatcaggg ctccattgac attaaagcaa aaacaccccg
     2521 aaacgaatgt ttcggggtgt ttttgtcact aaactcaatt ttttagggtg ttggggttgc
     2581 aagcggagca ggtgtacctg cgaagattac cagatctgaa accgcttcag tgtgtccggg
     2641 aacatcagct ttggctgtgg tcaggaagtc gtaaagcaat gagccttccc attttccacc
     2701 gccaaggatg tcagtgcctt tggattcggc atcggcacgg cgcgagccag aggtaatgcc
     2761 tgcataactg ctcaggtcaa gctgggcgag agagatggtc acatctgtcg agtggcagga
     2821 aacgcaggag agcgaccctg aaacccagaa attggattcc acaaagagcg gcatcacttc
     2881 cgcaaaagaa gattcacagt tcaagccgtt gatatcggtg aattggaagg cctcggtttc
     2941 agaggagcca gcttcaaccc atgcgccgat taaatccacg gcagctacac gacatttatc
     3001 agaaacgacc acttttccat caccggtttg tgcggcaggc atggtggcag gcagcagggt
     3061 ggggatcggg gttcgtacca caggctgcgc accgcggata caagtcaacg tgaacccgca
     3121 agagttgaca tacacaatgg caacccaggc cagcacgcca agcaggaaca cggttactgc
     3181 gccgtagata tactttctaa catcatcttt catatgggta cctccctact gcgccgcggg
     3241 cacggtaagc gtgcggatgt aattgaccaa atcccagcgt tcgcgtaccg agagattctc
     3301 gctcaaaggc ggcatttgcc cggagaattc agtatcaggg aagagggtgt tattgggatt
     3361 gaagatacca ttggaaatgg tcaggaacag gctgccatcg gtcttgctct ggacaagttc
     3421 actggtcaaa tcagcgggtt tcttcttcac cagaaaagca gagatggtgc cattgccctg
     3481 cccagtggct ccatggcaca tcaggcagtg aatggtgtac agttcggcac cacgcgcaat
     3541 ggaggtttca tcagcaggaa caggattgga tggggcgccc agattaggga tgtaggcggc
     3601 gccttccacc ggaatggatt gagcaggcac cggcagcggg ttttcctgcg cgctgtagga
     3661 gggttggatc tccatgaaga cgatccagtc aattttgatc acgtcgtagg agaagaccat
     3721 caacgcgcca accagcacgg caaggaccgc aaatacgaca acgagttgct taaagagtct
     3781 cataggtgtt gcgcctccgc aggtttcacg gattcagcgc ccattttgtt taaggcgtcc
     3841 gtaattttct tttcatcagc atgtggacat tccaccaaaa tggcgatctt gccgtcgctg
     3901 atctcgggca cgtatttcat cgggcggtag ttggggaaga aactatcgag aaatacgccg
     3961 aggaaagttg aaagcaacat acccagcatg gtcaactcaa agagcaccac cacagacggg
     4021 ggaccaggga cgaatgcttg tgtgctgact tgaatggggt atgggtaaat gtagggcgag
     4081 atgaaagcca aaaagaagcc tccagaaaag cccaaaatgg caccgcccat ggcaatgcgc
     4141 ggcacgtttg tccactggtg aggacggcca agcatggctt cagtaacggg cacaccagag
     4201 atgacgttca tgcaatcatc gtgaacgccc aaggtgcgaa gctgctcaat ggcattggca
     4261 gcaggttcga ggtcggggaa gaccgcaagc aggtcgacaa ttttcgattc agacatgtcg
     4321 tcctcctatt ccaattcgct gaccgcaacc acggtctcgc gtccaaattt cttgagttcg
     4381 tgtgccatct gaccttcctg cacttcccaa accgggataa gcggaatcaa cttggacgcc
     4441 gccatgtaaa gcaggatgaa caaagccact gtgccgatcc ccatggggac ttcaatgccc
     4501 ggggtgtaca tgcgccaggt gaagggcata cggttgatgg ttaccgagat cggaacgatg
     4561 atgtagcgtt caaaccacat accgacattg atgaccaacc caacgatggt gatggcccat
     4621 ggtgtggcgc gccactttgc gttccacaaa atagcccagg gaacaacaat gttacagaac
     4681 agcatgccaa accacatcca gcccaacggc ccagcctcat ggaagtgcag caattgctgt
     4741 gtccacttgt cgccgccgta ccattgaacc atgtaatcat tgaagaagaa gtaagcccaa
     4801 gccatggaga tgatgagcat gagtttgccg atggcatcga agtgttcacc gcgcacgaag
     4861 tatttcatat tcttcatggt gccgcggatc actgccaaca cgacggctgc cgcgcccata
     4921 ccggaatgca acgcgccgac gatgaagtac gggccaaaga tggtcgaaga ccaacctggg
     4981 cgggtggctg ctgcaaagtc ccaggacacg atggtgtgaa ctgagaacat gaccgggatg
     5041 atcgcccatg caaagatgtt cattgcgttg cgcagatggg tccattcacc ttctgtacca
     5101 cggaagccaa gtgcgagaac cttgtacaga ttcttgcgcc agccagtgga gcgatcgcga
     5161 gccatggcca catctggaat gagtggcagg aacaaataca tggtggagga aagcagatag
     5221 gtggtgatgg ccagcaagtc ccatgtgagt ggagaatgca ggttgggcca caacaagcgc
     5281 tgattggggt aaggcataag ccagtagaag agccacacac gacccatgtg catgaagatg
     5341 gaaaacccag cctgtaccag gccaaaggtg gtcatcaatt ctgcggcacg ggtgaatgga
     5401 cggcggaatt cagcgcggaa aacgcgcaaa atcgcagaaa tgaaggttcc tgcatggctg
     5461 ataccgatcc aaaacacggt gttgacgagg aaaatacccc agtagtcagg gcgcataaca
     5521 ccggcttgac cctgaccttt tgcgatcagg acgccccact tatagaagag gaaatacacc
     5581 accacgaaag agagaaaggc aaagatcacc cagtatttcc aggtgggttt ttccaacatg
     5641 gattccatga ccatgtcgtt catctttccg cgtggcagcg ggtcgagcat gaggtgtttt
     5701 tcgcgaagat gttcttcgtg cgagtgtgcc tcgtgatgtt ttgtatctga catgtttacc
     5761 ctcccttgaa gtaggtcacg ttgggaagcg tgccgagttc ttcaagatgt ttg
//
LOCUS       DAOQJC010000188         5779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_7958_length_5779_cov_9.821279, whole genome shotgun sequence.
ACCESSION   DAOQJC010000188 DAOQJC010000000
VERSION     DAOQJC010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7958_length_5779_cov_9.821279"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="PLE39_18130"
     CDS             complement(<1..862)
                     /locus_tag="PLE39_18130"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE70308.1"
                     /translation="MAVLSTKTTTAWAEVDEHGRLIIPPEVAQQYGLNPGSKVRLDEG
                     TNFVRMHRPVTHLTKVYIEPTVACNLDCITCFRNAWDQPIGRMTEETFESILNGLKKM
                     DPIPNVYFGGIGEPLFHPKTVEWIRRVKELGVKVELITNGTILTEKKSRELIDAGLDV
                     LWVSIDGATPESFTDVRMGAELPLILENLRRLFKMRKGGHFPQPEIGVAFVAMKRNIN
                     DLPKIIKMGHTFGARYFSVSNVQPATAEMQADRLYDRTIRNIAYLPSPVLPKLSLPKM
                     DFNEETQAALT"
     gene            1028..2152
                     /locus_tag="PLE39_18135"
     CDS             1028..2152
                     /locus_tag="PLE39_18135"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNE70309.1"
                     /translation="MADSRVAKFAKVLVEHSARVVPGDRILLEGTTAAEPLLRELYIQ
                     ILEKGGHPHLMMAIPGLMPFSQDELFLTYAKDTQLDFVPTFYKLAYEQFEGRIRIHSA
                     TNTKGTTNIDPAKLQRRGKATSVITEAQFRRGADGSFKWVTTLYPTDGYAQDANMSLS
                     EYEDFVFGAVHANEDDPVAFWRSVEGKQQSAVDYMKGKSQVILRGPNVDLTLSVKGRT
                     FLNSFGTFNMPDGEIYTGPVEDSVNGWVKFTYPANYGGTSVEGAELIFSNGRVTTAKA
                     DKNQEFLLKTLDIDAGARTLGEFAIGTNFDIQRFTGNILFDEKIGGSFHMALGAGYPE
                     TGAKNKSAIHWDMICDMRTDSEILVDGELFYKNGQFVFDK"
     gene            2277..3116
                     /locus_tag="PLE39_18140"
     CDS             2277..3116
                     /locus_tag="PLE39_18140"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNE70310.1"
                     /translation="MNINPTSRFYTLLKWFVPVAATFAFAAWMYLSPEGALGKLDAIG
                     YAVCHRIDVRSFQIDDRQLPLCARCTGEFYAAGVALLFQVFVSGKRSKLPSRGMIAVL
                     FIFFLAFGVDGSNSYLYLLKQTSSGALDKIPNLYVPNNTLRLFTGSGMGIALAAMLYP
                     VINQTLWRNLDDQPALEWKSLGGLIALVVVLNLLILTDSPIVLYPIAYISALGTLSLL
                     VIVFAILWIMIMRQDNSFETPSQLWLPFLSGLTLALLMILSIDMVRLQFTGTWSGFPG
                     MTG"
     gene            3145..3717
                     /locus_tag="PLE39_18145"
     CDS             3145..3717
                     /locus_tag="PLE39_18145"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNE70311.1"
                     /translation="MEMLLGIFSAFGLSASAGLNAYIPLLVVGVVAHYSPDTLALGKP
                     WDLLANPWILILLGVLVIIEMVADKVPAVNHINDVIQTVVRPVAGAIAFAASANVITD
                     ISPVLALACGLLMAGSVHAVKAVAVRPAVTATTGGAGNVPVSIAEDIAAFVTSLLAIL
                     LPIIVGTLLVILLALVLWWLWRRTDKAQTA"
     gene            3825..3980
                     /locus_tag="PLE39_18150"
     CDS             3825..3980
                     /locus_tag="PLE39_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70312.1"
                     /translation="MNDMPVSPEAPKKNNTPIIIGVVVVVLLCCCCAIAAGGWVYGDA
                     IVQSLGM"
     gene            complement(4004..4462)
                     /locus_tag="PLE39_18155"
     CDS             complement(4004..4462)
                     /locus_tag="PLE39_18155"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HNE70313.1"
                     /translation="MSGMKVLKKNLAEEVTWQYEGVVLSRAENAITLEALFNRDDMPF
                     MDIVLKRNDRFVETFYSDRWYNIFEIYDRDDGKLKGFYCNIGKPAVIEDDFVSYVDLA
                     LDLWVSTDGRQTVLDEDELEALNLDSELKQKVYAGLKELQGLFKSKNPPL"
     gene            complement(4450..5448)
                     /locus_tag="PLE39_18160"
     CDS             complement(4450..5448)
                     /locus_tag="PLE39_18160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE70314.1"
                     /translation="MLEVRDIFKTYENKPLLRGISFSVAQGETICLLGASGSGKSTLL
                     RMIAGLEFPESGHFLFNQIDLAQTPPHLRDFGLVFQDYGLFPHLNIFDNVAFGLKMRN
                     APTEAIKPRVSEMLELVNLTGFETRRVTDLSGGEQQRVALARALAPSPRLLMFDEPLG
                     ALDKSLKDDLLKQIRNILHQTKIPAIYVTHDQDEAFTIADRILLLHDGEIIRDGAPSE
                     VWNHPNSVHAARMLGVGNVIEGKIQKADGTKWKVESGISMFEVACKHDHSAGETISLL
                     IRQSGEGEEIKLTVEDVLFDRDQFQVKTRGGLVIHMKDAPRVGENIRVRVKVECLA"
     gene            complement(5450..>5779)
                     /locus_tag="PLE39_18165"
     CDS             complement(5450..>5779)
                     /locus_tag="PLE39_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70315.1"
                     /translation="IPERLRQAASTLGASPFRVWQTVDFPILSRATLSAATFAFTVSL
                     GEFGATLLLTRPEYPTIPIAIQRFLSQPGGLNFGQAMAMATLLMLLTTLSILAIEKLR
                     LSDSGEF"
BASE COUNT         1354 a         1410 c         1457 g         1558 t
ORIGIN      
        1 cagtaagagc cgcctgggtt tcttcgttga agtccatttt gggcaggctg agttttggca
       61 gaacagggga gggcaggtac gcgatattgc ggatggtgcg atcatacaaa cggtcggctt
      121 gcatttcagc tgttgcaggc tgaacattgc tgacagagaa ataacgggcg ccgaaggtgt
      181 gtcccatttt gatgatcttc ggcaggtcgt taatattgcg tttcatggca acaaaggcca
      241 cgccaatttc aggctggggg aaatgcccgc ctttgcgcat tttgaatagt cggcgcaggt
      301 tttcgaggat gaggggcaat tccgcgccca tgcgcacatc ggtgaaactt tcgggagtag
      361 cgccgtcgat ggatacccac agaacatcca gaccggcatc aatcaattcg cgtgacttct
      421 tttcagtgag gatggtgcca ttggtgataa gctcaacctt gacgcccaat tccttgacgc
      481 gccgaatcca ttcgactgtt tttgggtgga agagtggttc tccaatgccg ccaaaatata
      541 cattggggat cggatccatt ttctttaacc cgttgaggat actttcgaaa gtttcctcgg
      601 tcatacgacc gatgggttga tcccatgcat tgcggaagca ggtgatgcag tcgagattac
      661 atgcgacggt gggctcaatg tagacctttg tgaggtgcgt caccgggcga tgcatgcgga
      721 caaaatttgt gccttcgtcg aggcggacct tcgagccggg attcagccca tattgctggg
      781 caacttcagg cggaatgatg aggcgtccgt gttcgtccac ttctgcccag gctgtggtag
      841 tttttgttga tagaacggcc atgctttctc cttacaaata aaccaattac cacctatcat
      901 atccattttt gcaaaaaatt catgtgacaa aagttacatt ttccatatta cgaagtattt
      961 tgcccttctg caagagtgga aataatgagg tgatataatt tcaccattcc attttaggag
     1021 catttcagtg gctgattctc gtgttgcaaa atttgcaaaa gttttagtgg aacattctgc
     1081 acgtgttgtg cccggcgacc gtattttgct cgaagggaca acagcggcag agccgttgct
     1141 gcgggagtta tatattcaga ttctggaaaa aggcggtcac cctcatttaa tgatggcgat
     1201 tcccgggttg atgcctttca gtcaggatga attgtttttg acctatgcca aggatacgca
     1261 acttgatttt gtgccgacat tttataaatt ggcatatgaa caattcgaag gacgtatccg
     1321 tattcattcg gctacaaata caaaagggac aacgaacatt gaccccgcca aacttcaacg
     1381 gcgcggcaag gccacatctg tgatcacgga agcgcagttc cgccgtgggg cagatgggtc
     1441 tttcaaatgg gtgaccaccc tctacccgac cgatggttat gcccaagatg ccaatatgag
     1501 cctgtctgag tatgaagact ttgtgtttgg tgctgtacat gccaatgaag atgatcccgt
     1561 tgcattctgg cgaagtgtgg aaggcaagca gcaatctgca gtggactata tgaagggcaa
     1621 gagtcaagta attttgcgcg gccccaatgt agacctgacc ctttccgtaa aagggcgcac
     1681 tttcctgaat tctttcggta catttaacat gcctgacggt gagatttata ccggtcccgt
     1741 ggaagactct gtgaatggtt gggtgaagtt cacttatcca gccaattacg gcggcaccag
     1801 tgtggaaggc gctgagttga tcttctccaa tgggcgtgtc actacggcca aggctgataa
     1861 gaatcaggag tttttgctga agacacttga tattgatgcg ggggcccgta cactgggtga
     1921 atttgccatc ggcaccaatt ttgatattca aagatttacc ggcaacattc tgttcgatga
     1981 aaagatcggc ggttccttcc atatggcact tggggcaggt tacccggaaa cgggtgcaaa
     2041 gaataagagc gcgattcact gggacatgat ctgcgatatg cgcactgatt cggaaattct
     2101 tgtggatggt gagttgttct acaagaatgg ccagtttgtg ttcgataaat agttttaacg
     2161 ataatcaagc tgtatatttg aaggttgagg aatttcctca accttctttt gtttgttcta
     2221 catctgaaat tttcgctttc ttgccttccc gtcttattcc ccatataatg tgaacgatga
     2281 atataaatcc aacatcacgt ttttatacat tgttaaagtg gttcgtgcct gttgcggcaa
     2341 cttttgcttt cgcggcatgg atgtatcttt cccctgaagg cgcacttgga aaactggacg
     2401 ccattggtta tgcggtctgt catcgcattg atgtgcgctc cttccagatc gatgaccgcc
     2461 agcttccgct gtgcgcgcgt tgtacgggtg aattttatgc ggcgggtgtt gcgctgctgt
     2521 ttcaggtctt tgtcagcggt aaacgcagca aactgccttc ccgcggcatg atcgctgttc
     2581 tgttcatttt cttccttgcc tttggtgtgg acgggagcaa ttcgtacctg tatctgctca
     2641 aacaaacttc atcaggtgcg ttggataaaa ttccaaactt gtatgtcccc aacaatactt
     2701 tgcgcctttt tacaggcagc ggcatgggga tcgctctcgc agccatgttg taccctgtca
     2761 ttaaccagac tttgtggcgt aacctggatg atcagcccgc tctcgaatgg aaatcactgg
     2821 gcggcctgat cgcactggtc gttgtcctca atctgttgat tcttacagac agccccattg
     2881 ttttgtaccc gatcgcctac atcagtgcct tgggtaccct ttctctgttg gtgatcgtct
     2941 ttgccatttt gtggatcatg atcatgagac aggataacag ctttgaaacc cccagccagc
     3001 tctggctgcc ttttctttcc ggtctgaccc ttgctttgtt gatgatcctc agcattgata
     3061 tggtccgcct gcaatttaca ggtacgtgga gtggcttccc cgggatgacg ggatagatgg
     3121 cgtgaaaaaa taaaggagag aaaaatggaa atgttgctcg gtatattttc tgcttttggc
     3181 ctttccgcca gtgcggggct caatgcctat attccgcttt tggtggtggg ggtggtggct
     3241 cattactccc ctgatacgct tgccctcggc aaaccctggg acctgctcgc caacccatgg
     3301 attctcattc tgttgggggt tctggtcatt attgaaatgg tggccgataa agttcccgca
     3361 gtcaaccaca tcaacgacgt catccaaacc gtcgtccgcc ctgtggcggg ggccattgct
     3421 tttgccgcca gtgcaaacgt catcaccgac atcagccccg tacttgcgct ggcttgtggt
     3481 ctgctcatgg caggcagtgt ccatgcggtt aaggcggtgg ccgtgcgtcc ggcggttaca
     3541 gccactacag gcggggcagg gaacgtcccc gtttccattg ctgaggatat cgccgcgttc
     3601 gtcacatccc tgctggcaat tcttctcccg atcatcgtcg gcaccctgct ggtcattttg
     3661 ctggcactag tactttggtg gctctggcgc cgcacagaca aggcgcaaac tgcctgatat
     3721 aatcgggcca atcaaattcg tactgctgac aaaaaaagca aaaatctgtt cagcacgcgc
     3781 aaaattcgtg tcgcgcgatt caattatcaa aaggagtaga aatcatgaat gacatgcctg
     3841 tttcccctga agcgccgaag aaaaataaca cccccattat tatcggcgta gttgttgtag
     3901 ttttgctttg ctgctgctgt gccattgcgg caggcggctg ggtctacggc gacgccattg
     3961 tccaatcgct tggaatgtaa ttgactgtaa aaaatcctcc actctaaagt ggaggatttt
     4021 ttgatttaaa caacccctgt aattctttca gcccagcata cactttctgc tttaattcag
     4081 aatccaaatt caacgcttcc aattcgtctt cgtccaaaac cgtttgcctg ccatctgttg
     4141 aaacccacag gtccagtgca aggtctacat atgaaacgaa atcatcttcg atcacggcgg
     4201 gtttgccgat attgcagtag aagcccttca gttttccatc atcgcggtcg taaatttcaa
     4261 agatgttata ccagcggtcg gaataaaagg tttccacgaa acggtcatta cgtttcagca
     4321 caatatccat gaacggcata tcgtcgcggt tgaacaacgc ctccagcgta atggcatttt
     4381 ctgcgcggct caacaccacg ccttcatatt gccacgtcac ttcttctgcg aggtttttct
     4441 tcaaaacttt catgccagac attctacctt aacccgcaca cggatgtttt caccgactcg
     4501 cggagcgtct ttcatgtgga tgacaagccc gcctcttgtc ttaacctgaa actgatcccg
     4561 atcaaaaagc acatcctcca ctgtaagttt tatctcctca ccttcacccg actgcctgat
     4621 tagcaacgaa atagtttcac ctgccgaatg atcgtgtttg caggctactt caaacatact
     4681 gatcccactt tctactttcc actttgtacc atctgccttc tgtatttttc cctcaataac
     4741 gttccccacc cccaacatcc gcgccgcgtg gacagagttt ggatgattcc acacttcact
     4801 tggcgcgccg tcgcggatga tctcgccgtc gtgcaaaagc aaaatgcggt ctgcaatggt
     4861 gaaggcttca tcttggtcgt gtgtgacata aatggcggga atttttgttt gatgcaggat
     4921 attgcgaatc tgcttcagca gatcatcttt caaagatttg tccaaagccc caagcggctc
     4981 atcgaacatc aacaagcgcg gacttggcgc aagggcgcgg gcgagtgcca cacgctgctg
     5041 ctcgccgcct gaaaggtcgg tcaccctgcg ggtttcaaag cctgtcaaat tgaccaactc
     5101 caacatttca gagactcttg gttttattgc ttcggtgggg gcgtttctca tcttcaaccc
     5161 gaaagccacg ttgtcaaaaa tgttcaggtg aggaaaaagc ccatagtctt gaaagaccaa
     5221 gccaaagtcg cggaggtgag gcggtgtttg ggcgaggtcg atctgattaa acaggaaatg
     5281 tccgctttca ggaaactcca aaccagcgat catccgcaag agagtggatt ttccactgcc
     5341 cgatgcgcca agcaggcaaa tggtttcgcc ctgtgccacg gaaaatgaaa tgccgcgcag
     5401 aagcggtttg ttttcgtagg ttttgaaaat gtcgcggact tcgagcattt tagaattctc
     5461 cagagtcgga aagacgtagt ttttcaatgg cgagaatcga aagcgtggtg agcaacatga
     5521 gcagagttgc cattgccatt gcctgcccaa aattgagacc gccaggctgg gaaagaaaac
     5581 gttgaatggc gatcgggatg gtcgggtact cggggcgggt gagcagcagg gtcgcgccga
     5641 actcgccgag cgatacagtg aaagcaaaag tagcggcgct cagtgtggca cgggaaagaa
     5701 tcggaaaatc cacggtctgc caaacacgaa acggcgatgc gcccaaagtg gatgcggctt
     5761 gacgcagacg ttcagggat
//
LOCUS       DAOQJC010000189         5753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_8010_length_5753_cov_8.700597, whole genome shotgun sequence.
ACCESSION   DAOQJC010000189 DAOQJC010000000
VERSION     DAOQJC010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8010_length_5753_cov_8.700597"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PLE39_18170"
     CDS             <1..696
                     /locus_tag="PLE39_18170"
                     /inference="COORDINATES: protein motif:HMM:NF014042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HNE70316.1"
                     /translation="ETWNTIANIEKLGGPNWFRLGDQDIATHLERTRRLKGGETLSQI
                     TKDFCKAWGIQPTILPMTDAPVRTIVDSDEGELAFQEYFVHRQCGPKVKGFRFDGVEA
                     AEPAFGVREAVESADAIIICPSNPWVSVDPILRVIPLPKKKIVAVSPIIGGKTVKGPA
                     AKMYAELGIEPSALAVAEHYRDLLSGFVLDNVDAHLSDKIRTRTLVTDTLMNSLTDRA
                     RLAMDVLHFIGSL"
     gene            699..1304
                     /gene="cofC"
                     /locus_tag="PLE39_18175"
     CDS             699..1304
                     /gene="cofC"
                     /locus_tag="PLE39_18175"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HNE70317.1"
                     /translation="MTLWAIVPVKPLRRGKSRLAGMLTEDERTALNQELLERTLKTLS
                     TLKELDQVLVVSRDPAALTIARNHGAKTVQEDGQPHLNTALMRATVVAQVHATQGVLI
                     LPADLPLLTPDDVLTLIDRAAKPPVVVIAPDRHGKGTNALLMVPAGQIEYDFGEGSFQ
                     RHCERTKQSGARLEIVELPSLGLDLDLPEDLEMVRKMEALK"
     gene            1370..2368
                     /locus_tag="PLE39_18180"
     CDS             1370..2368
                     /locus_tag="PLE39_18180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNE70318.1"
                     /translation="MTERVALYLQDSHDLRDGLDYAKYAEEKGFEAVWQAESRLVRDA
                     IVPMAGYAAVTNKIKVGSGVINNWTRNIGLLAATFLTLDDLAPNRVICGLGAWWDPLA
                     KNVGIDRRKPLTAMKETVIVMKKLLNMERVSFDGEFIHVNGIELDVVYGRREPRNVPI
                     MIGATGDQMMELTGEVADGAVLNYCVAPEYNDRAMELLEKGLRKSDRKMENFDRPQLI
                     VCSVDEDHDKAINYSKMLLCQYIAQQPHIAKASNVSDDVVHQIQSILGWPATKEQVMK
                     AKDLVPDELVHKITASGTPSEARAKVAEYTKRGCTCPILYPVGGNFKLLIDTFAQS"
     gene            2474..3088
                     /locus_tag="PLE39_18185"
     CDS             2474..3088
                     /locus_tag="PLE39_18185"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE70319.1"
                     /translation="MKPNSPTLSPQETRERILEAAVTVFASKGYHDTKVDDIVSESNT
                     SKGSFYFHFPSKQDIFLALVDTFADLLETRLKDRLSASTSGLERVELALRVCLETFGQ
                     YRALAKIALVQATGLGAAFEEKRRTVNERFIQFIKENLDAAVADGSIPALDTEVTASA
                     WIGALNEVVMRWIYTGQPDPERSLPALRKIFLLSLEIPIERINQ"
     assembly_gap    3104..3203
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3239..4195
                     /locus_tag="PLE39_18190"
     CDS             3239..4195
                     /locus_tag="PLE39_18190"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HNE70320.1"
                     /translation="MRRIRSLLAFVIIALLAAACAPATTAPQETVTLKIAVLPIIDTL
                     PMYVAQQEGLFAKHGVNVEFVPVASAPERDQLVGAGQADGMVNETISVMLFNKEKVQV
                     QAVRYALRPTENAGHFFIIASAKSGITDAQGLKGIEIGVSQGTIIEYVTERLLQAEGL
                     TSDEIKTIAVPKIPDRMALLASGELNAGVLPDPLGALAVQQGAVIVLDDSRHPEYGFS
                     VISFRKEVIDANPAAIKAFLAAIEEATTMLNSDSKKYTSVLSDQKLVPPPLLASYAVP
                     PFPTAGVPTEAEWSDALAWVKEKGMLTTDVSYADSVNATFLP"
     gene            4266..5021
                     /locus_tag="PLE39_18195"
     CDS             4266..5021
                     /locus_tag="PLE39_18195"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE70321.1"
                     /translation="MITVSSLTFNYANNTPIFQDFNWQVQRGETWAILGSSGCGKTTL
                     LYLLAGLRFPSKGQIEIDGERLTRPRPHSGLILQDYGLLPWATVQENAGLGLKVREFY
                     GEDGKHAPKNFQPENNVAHWLTRLGLSEVADKYPSQISGGQRQRTAIARTLALSPDLL
                     LMDEPFSSLDAITREDLQNLTLSLCEEQKLTLVIVTHSIEEAASLGGKILLLGSSPRV
                     FENPTVRQGDHRNSQEYRDLCNLLWKEMRNETA"
     gene            5008..5748
                     /locus_tag="PLE39_18200"
     CDS             5008..5748
                     /locus_tag="PLE39_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE70322.1"
                     /translation="MKRRDLLFSAAGLVLIWQVAAMLIQRPILPAPWEVLVVFVQELG
                     NGLVTHFFYSLWRVTAGMALSVLIAAPVGLAVGGSKRLDAIFSPIIYLLYPIPKVVFV
                     PVVLLFLGIGDVAKITIIFLILFFQIVVLVRDQAAGLAPQLIQSLQSLGAGRRALFRF
                     VYLPASLPAILTALRQSIGTAVAVLYITELFATKYGLGYYIYYNGSTLFHYPAMYAGV
                     IAMSLLGLGMYYTVDWLERKWCKWKFVS"
BASE COUNT         1386 a         1534 c         1461 g         1272 t
ORIGIN      
        1 gaaacctgga acaccatcgc aaatatcgaa aaactgggcg gaccgaactg gttccgcctg
       61 ggcgatcagg atatcgctac tcaccttgaa cgaaccagaa ggctaaaagg aggtgaaacc
      121 ctttctcaga tcacaaaaga tttttgcaaa gcgtggggaa ttcagccaac gattctgcca
      181 atgaccgacg cacccgtacg cacgatcgtg gattcagatg aaggcgagtt ggcctttcag
      241 gaatattttg tccacaggca atgcgggccg aaggtcaaag gttttcggtt tgacggagtc
      301 gaagccgctg aaccagcttt cggcgtgcgc gaagcggtcg aatcagccga tgcgatcatc
      361 atttgcccgt cgaatccatg ggtgagtgtc gacccaattt tgcgggtcat tccgcttcca
      421 aaaaagaaaa tcgtagcggt ttccccgatc attggtggaa aaaccgtgaa aggccctgcg
      481 gcaaagatgt acgccgaatt gggcattgag ccttccgcgc tggcggtggc ggaacattat
      541 cgagaccttt taagtggttt tgtgttggat aacgtggatg cacatttatc ggataaaatc
      601 aggacaagga ctttggtaac agatactctt atgaattcac ttacagatcg cgcccgattg
      661 gcgatggatg tgctacactt tatcgggagt ttataaacat gacactttgg gcaattgtcc
      721 ctgtaaaacc tttacgccgg gggaaatccc gcctggcggg catgctgaca gaagatgaac
      781 gaactgcttt aaatcaggaa ctgctggagc ggactctaaa aacgctctca accctgaaag
      841 aattggatca agtgctggtg gtcagccgcg accctgccgc attgaccatt gcacgcaacc
      901 acggcgccaa gaccgtgcag gaagatggac agccgcacct caatacagct ttgatgcgtg
      961 caaccgttgt ggcacaggtc catgccacgc agggggtttt gattctgcca gccgacctgc
     1021 ccctgctcac ccctgatgat gttttgacct tgatcgaccg cgcagccaaa ccgcctgtgg
     1081 ttgtgatcgc gccagacagg catggcaagg gcacaaacgc cctgttgatg gtccctgccg
     1141 gccaaattga atacgacttt ggcgagggtt cattccagcg ccactgcgaa cgcaccaaac
     1201 agtcgggagc acggctggag atcgtagaac tcccctcgct cggcctcgac cttgacctgc
     1261 ccgaagacct tgaaatggta cgaaaaatgg aagctttaaa gtgatcggaa attaattggc
     1321 ccagaaggtc aatgacaccc aaatctattt ttctcaagga gaaaaaccca tgaccgaacg
     1381 tgttgccctg tacctgcaag attcgcacga cctgcgcgat ggtttggatt acgccaaata
     1441 cgcagaagaa aaaggattcg aagccgtttg gcaggcggag agtcgtttgg tgcgcgatgc
     1501 cattgtgccg atggcgggtt acgctgctgt aaccaacaag atcaaagtcg gctcgggtgt
     1561 catcaacaac tggacccgca acattggcct gctggccgcc acttttctga cactggatga
     1621 ccttgctcca aatcgtgtca tctgtggatt gggcgcatgg tgggacccgc tagccaaaaa
     1681 cgtaggcatt gaccgccgca agccgctgac cgcgatgaaa gaaacggtca tcgtgatgaa
     1741 gaagctgctc aacatggaac gcgtttcctt cgatggtgag ttcattcatg tcaatggaat
     1801 tgaattggat gtggtctatg gccgccgcga accgcgcaat gtccccatca tgatcggcgc
     1861 cacgggcgac cagatgatgg aactgacagg cgaggtggca gacggtgctg tgctcaacta
     1921 ttgtgtagcc cccgaatata acgaccgcgc catggaactc cttgaaaaag gcctgagaaa
     1981 atcggaccgc aagatggaaa acttcgaccg cccgcagctc atcgtctgct cggtggatga
     2041 agaccacgac aaagccatca actattccaa gatgttgctc tgccagtaca tcgcccagca
     2101 gcctcacatt gccaaggcgt ctaatgtctc cgacgatgtt gttcatcaga tccagtccat
     2161 tttaggctgg cctgccacca aggaacaggt gatgaaagcc aaagacctcg tccccgatga
     2221 actcgtccac aagatcacag cttcaggtac accctccgaa gcccgcgcca aagtcgctga
     2281 atacacgaag cgcggatgca cctgccccat tctctaccct gtcggcggca atttcaaact
     2341 cctcatcgac accttcgcac aaagctagcc cgcaggcaaa tgtcataagc aacacatgag
     2401 ccacgtcact tgacgtggct ttttgattca actatcctac ctgccgatag accgaccagt
     2461 cggtctaggg atcatgaaac caaattcacc caccctctcc ccgcaggaaa cccgtgaacg
     2521 catccttgaa gcggccgtta cagtctttgc cagcaaaggc tatcacgaca ccaaagtgga
     2581 tgacattgtc agcgaatcca acacctccaa aggctcgttc tactttcact tcccaagcaa
     2641 acaggatatt ttcctcgcgc tcgtggatac ctttgccgac ctgctggaaa cacgactcaa
     2701 agaccgcctc tctgcttcga cctctggcct ggaacgtgtt gaactggcat tacgcgtctg
     2761 tttggagacc ttcggccaat accgcgccct ggcaaagatt gcccttgttc aggccactgg
     2821 ccttggtgct gcttttgaag aaaaaaggcg cactgtaaac gaacggttca tccaatttat
     2881 caaggaaaac ctagacgcag ccgtagcgga tggaagcatc cccgcgttgg ataccgaagt
     2941 gaccgccagc gcgtggatcg gcgccctgaa cgaagttgtc atgcgttgga tctacacagg
     3001 ccagcccgac cctgaacggt cactgccagc cttgagaaaa atatttttgt taagtcttga
     3061 aatcccaata gagagaatca atcaataaga tgtaacgcgg actnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnaagtccg cgttacaaaa caccttagga gaaaaagaat
     3241 gagaagaatc agatccttgt tggcattcgt catcattgca ttattggctg cggcctgcgc
     3301 gcccgctacc acagcaccgc aggaaacagt gaccttgaag atcgccgtcc tgcccattat
     3361 cgacacactg ccgatgtacg ttgcgcagca ggaaggtcta ttcgccaaac atggcgtcaa
     3421 tgtagagttc gtgcccgtag cctctgctcc ggagcgtgat caacttgttg gggctggtca
     3481 ggccgacggt atggtcaacg aaacgatttc ggtcatgttg ttcaacaagg aaaaggtgca
     3541 agtacaagcg gtccgctacg cattgcgccc caccgaaaat gcaggccatt tcttcatcat
     3601 cgcctccgcc aagagcggga tcaccgatgc gcaaggcttg aagggaattg agatcggtgt
     3661 ttcgcaaggc acaatcattg aatatgtcac cgaaagactc ttgcaagctg aagggttgac
     3721 gagcgacgag atcaaaacca tcgccgttcc aaagattccc gaccgaatgg ccctgctcgc
     3781 atcaggtgaa ttgaacgcag gcgtgctccc agacccgcta ggcgcactgg cagtgcagca
     3841 aggcgcagtt attgtccttg acgactccag gcaccccgaa tatggtttca gcgtcatctc
     3901 cttccgcaaa gaagtcattg atgcaaatcc cgctgccatc aaggcgtttc ttgcagccat
     3961 cgaagaagcg acaaccatgc tcaacagcga ctcaaaaaaa tacaccagcg ttttgagcga
     4021 ccagaaactt gtcccaccgc cgctgcttgc cagttacgca gttccgccct tccccaccgc
     4081 aggtgtgccc accgaagcgg aatggagcga cgcactggca tgggtgaagg aaaagggaat
     4141 gctgaccaca gatgtttcct atgcagattc ggtcaacgca acgtttcttc cttaaccgat
     4201 aactgaaccc gttataatga attgacagtc acccgtggag gtgactgtca atttttgaag
     4261 cagacatgat cacggtttct tcactcactt tcaattacgc caacaacacc cccattttcc
     4321 aggatttcaa ctggcaggtt caacgcgggg agacttgggc catcctcggc tcttcgggct
     4381 gcggaaaaac aaccttgctt tatctgctgg ctggcctgcg ttttccctcc aaaggccaaa
     4441 ttgaaattga cggagaacgc ctcacccgtc cgcgcccgca cagtggattg atcctgcagg
     4501 attatggttt acttccatgg gcgaccgtgc aggagaatgc aggtcttgga ttaaaggtcc
     4561 gcgagtttta cggagaagat gggaaacacg cgccaaaaaa ctttcagcct gaaaacaatg
     4621 tggcgcactg gctcacgcgg ctgggattaa gcgaagtcgc cgacaaatac ccatctcaaa
     4681 tctctggtgg gcagcgtcaa cgcactgcga tcgcgcgcac gctggcactt tcgccagacc
     4741 tgctgttgat ggacgagcca ttttcatcac ttgatgccat cacccgcgaa gacctgcaaa
     4801 acttgaccct ctctctgtgc gaagaacaaa aattgactct ggtcatcgtc acgcattcca
     4861 tcgaagaggc tgcatcgctg ggtgggaaaa tcctcctgct tgggtcatca ccacgcgttt
     4921 ttgagaatcc aaccgtgcgg cagggcgatc accgtaacag tcaggaatac cgcgatctgt
     4981 gcaatttgtt atggaaggaa atgcgcaatg aaacggcgtg accttctttt ctctgcggca
     5041 ggccttgtac tcatctggca ggttgccgcc atgttgatcc agcgtcccat tttgcctgca
     5101 ccgtgggaag tgctggtcgt tttcgtgcag gaactgggta acgggttggt cactcacttt
     5161 ttctacagcc tgtggcgtgt cacggctggg atggcgctgt cggtgctgat cgctgctcct
     5221 gtcgggctgg cagttggcgg gtcgaaacga ctcgatgcaa ttttttcccc gatcatttat
     5281 ttgctgtatc cgattcccaa ggtggtcttt gtccccgtcg tgttgttatt cctcggcatc
     5341 ggtgacgtgg ccaagatcac catcatcttt ttgatcctgt tctttcagat cgtggtgctc
     5401 gtgcgcgatc aagcggctgg gcttgcgccg caattgatcc aaagcctgca aagtctcggg
     5461 gcgggccgca gggcgctgtt ccgttttgtt tatttgcccg ccagcctgcc cgcgatcctg
     5521 accgcgctgc ggcaatccat tggcacagcg gtggcggtgt tgtacatcac agaattgttt
     5581 gcaaccaaat atgggcttgg gtattacatc tactacaacg gcagcacact gtttcattat
     5641 ccagccatgt atgcaggtgt gatcgcgatg agtctgctcg gccttggaat gtactacaca
     5701 gttgattggc tggaaagaaa atggtgtaaa tggaagtttg tgagttgaag gtt
//
LOCUS       DAOQJC010000190         5650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_8241_length_5650_cov_9.059875, whole genome shotgun sequence.
ACCESSION   DAOQJC010000190 DAOQJC010000000
VERSION     DAOQJC010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8241_length_5650_cov_9.059875"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PLE39_18205"
     CDS             <1..458
                     /locus_tag="PLE39_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70323.1"
                     /translation="IMRAVGWLLFMVMWIPFTLMFLNMPNGNSNYDSLIGLPKTMALF
                     LGITIGLAIISTALLVGSSVVGWLIGRMVLARGESGTARIIAIQPTGTRVNNYYYGMR
                     FTLEVQSFGEAFQSHAERLVPMHDMTKYQIGMTVNVKYDPMTKTVAMVD"
     gene            810..1295
                     /locus_tag="PLE39_18210"
     CDS             810..1295
                     /locus_tag="PLE39_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70324.1"
                     /translation="MKKKISILIGGLLAVAMLFGAVSVSIAYAQSGSLTPFSDHGGPG
                     HGGRGLGQAELEAAAKVLNMTADELSTALQAGKTLEELATAAGVDIQDVKDAIMAVHA
                     EEIRARIEAGVADGSISQEKADWLLEGLDKGFLDGPGFGFGPGGHHDGQPAQPAQTTT
                     P"
     gene            complement(1381..2145)
                     /locus_tag="PLE39_18215"
     CDS             complement(1381..2145)
                     /locus_tag="PLE39_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70325.1"
                     /translation="MNSSTESMRWETKVFIFLMISSCLFTTGICACFSSPLILADEDV
                     REELQQFGFSGPAEKCPQIPTGWVIAKENTFDKNLNLWPLGKNSDDYDITNLGMADGV
                     LHYEVEALQGVYSRQFPGLDKNLWDFYLTAQIQKVSGPRDARYGVIFRVRGEKQLFFS
                     IRDSGSVRVAVHEYGGWLDPLFTGYSRYVNAEESNELVVIAQGDIYTFCVNGYIVGSV
                     KTTKYMSGKFGLGFELNGGEKAIFEIDNFNTYAPEE"
     gene            complement(2274..3404)
                     /locus_tag="PLE39_18220"
     CDS             complement(2274..3404)
                     /locus_tag="PLE39_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70326.1"
                     /translation="MKNKLQIIISVILLMAVTLACGGTAATPAAQDPNIVINTAIAGT
                     QQANALAQATVNANVLTAMPATPTPGPSVDYVALTEEELAALIDEAVAEAVAATEATT
                     SSVYTTTSDDTVTTEEVTYVYDYYYYADYYVEYAEDLMAQYYELYSDLAYQAIAEMQA
                     IEAELNQLNETLVSIDQSLQEISTTLEQGLALAEESIAQLENAAQEAQVKAEAVKSQA
                     QDMIEVIKADQQGRVDEISSIQPHDVPTDQLAVFQSAFDFIDAANLAMGDNKLSHDEL
                     MNLAQLGKNAQAGFMELGKNGGGPGMPGGGTLDFTQLSGRFGDITTQLARGEMPHARK
                     GLGEFELSLGGRPGGGGPGLPGGGGPGGPGAPGGGGPGGPRP"
     gene            complement(3478..4800)
                     /locus_tag="PLE39_18225"
     CDS             complement(3478..4800)
                     /locus_tag="PLE39_18225"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNE70327.1"
                     /translation="METNLFLNSSVDADITPFGFIWGMLRSPLHTLADLARDNGDIAH
                     VKMPKRDFYLLNHPEFIEEVLVKQQANFIKGPSLQRARIILGEGLLTSEGDEHLSQRR
                     ALQPAFHRTKMEEYLPVMGLNTFEHLTRWQHGSVVDMSDEMMRLTLNIALWSFFGSAP
                     EGSVERVSKAMKTLVRLFPLAQLPLPEATRMLFPKFRQAKTDLNIVTETLLANPHSEQ
                     AKRALITILKNEHHDQFTDEQLHAHVLTFLLAGHETTALLLSWCWDMISQDPFVQGKL
                     QAEADAVLGDRFPTSEDIKSLNYTRMVVKETLRMRPPAWALGRQAVQDCTIGGQDIKA
                     GSVVLMSQWVTQHDPRFYDSPLQFRPERWSEIERLPFPRYAFFPFGGGNRVCIGEHFA
                     MTEAIAILAIISRHWQILPTQAKHATPKPSVTLRPNKGVKVLIEKRDV"
     gene            complement(4943..5581)
                     /locus_tag="PLE39_18230"
     CDS             complement(4943..5581)
                     /locus_tag="PLE39_18230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE70328.1"
                     /translation="MLKIIIADDHQIVRRGLRMTIEAEKDLKVLAEAVNGTEVLALIK
                     KHSPDVVLMDLQMPELDGVETLKAIRPEFPELPVLILTSFTDDAHVYAALRAGASGFL
                     LKEMGGDELVEAIRGAAKGKPQLHPDIAKRLMAHAPMPEDPFGKLTEREREILKLLGR
                     GMSNKEIAVKLTLTEMTVKGYVSDVLMKLGVSDRTQAALMAVRFGLVKPEEL"
BASE COUNT         1234 a         1428 c         1537 g         1451 t
ORIGIN      
        1 gaatcatgcg tgctgttggc tggttgctgt ttatggtcat gtggatcccc tttaccttga
       61 tgttcctcaa catgccgaac ggcaactcaa actatgactc tctcatcgga ctgccgaaga
      121 ccatggcatt attccttggg atcacgatcg gtctggcgat catttccacg gctttgctgg
      181 tcggttcctc cgtggtcggc tggttgatcg gacggatggt gctggcgcgg ggggaatctg
      241 gcacggcccg tatcattgcc atccaaccca cagggacgcg cgtgaacaat tactactacg
      301 gcatgcgttt tacgctggag gtgcaatcgt tcggtgaagc ttttcaatca cacgcggagc
      361 gcttggtgcc catgcacgat atgaccaaat accagatcgg catgaccgtc aacgtaaaat
      421 atgacccgat gacaaaaacg gttgcaatgg tggattgata gtttgttttg aagatggaca
      481 ggttttgaaa atgggaaaca ttttcaaaac ctgtttttga ttctccctgc tttttgcatg
      541 ggcaagtcgg attgccaatc cgacttacct tgggcgcgca tcgtggcaac tcttggaaag
      601 tgtctgaaaa gcggactttc gcagatgggt ttgaaattcg cggcgaaatg tttggttggc
      661 tgttaagaac ggcagaacca ttaatacttt ctaatttcaa acacacaatt tccatatacc
      721 ccggtcacag cttgattaca tgggcggaat atcatttgcg aaggttggag gcgctccaac
      781 cacagatcaa aaataaagag gtaaaaaata tgaaaaagaa aatttcaatt ctgatcggcg
      841 gtttattggc ggttgcgatg ctgttcggag cggtgagtgt gagcatagcc tatgcccaga
      901 gcggaagttt gactccgttc agcgatcacg gcggccccgg tcacggtggg cgtgggctgg
      961 gtcaggcaga actcgaagcg gccgccaagg tactcaacat gaccgcggat gaactgtcca
     1021 ctgcgctgca ggcaggcaag acacttgaag agcttgccac cgccgcaggt gtggatattc
     1081 aagatgtgaa ggatgccatt atggcggtcc atgctgaaga gatccgcgcc cgcatcgaag
     1141 ccggtgtggc cgacggcagc atctcgcagg aaaaagccga ctggttattg gaaggtctgg
     1201 ataaaggctt cctggatggt cccggctttg ggttcggccc tggcggtcat cacgatggtc
     1261 agcctgccca acccgcacag accacaaccc cataaccatt atgaagccat aagataaaga
     1321 aaggagttaa aagaaacaga tgaggcgcac tcctccgcct catctgtttt gattcgtgaa
     1381 ttattcctct ggggcgtagg tgttgaaatt gtcgatctca aaaatggctt tttcaccgcc
     1441 gttcaattca aaaccgagac caaactttcc tgacatgtat tttgtggttt tgacgcttcc
     1501 aacgatgtat ccattcacgc agaaagtgta gatgtctccc tgcgcgatca caaccagttc
     1561 attgctctct tctgcattga catacctgga ataccccgtg aacaagggat ccagccagcc
     1621 gccatattca tgcaccgcca ctcgaacact gcccgaatca cgaatggaga aaaacaattg
     1681 cttttctccg cgtacccgaa aaataacgcc gtatcttgca tcgcgtggac cagatacttt
     1741 ttggatttga gcagtgagat aaaaatccca tagattttta tccagcccgg ggaattgacg
     1801 ggagtacacc ccctgcaatg cttcaacctc ataatgaagg acgccgtcgg ccattcccag
     1861 attcgtaata tcatagtcgt cggagttctt tcccagcggc catagattca ggtttttatc
     1921 gaaggtattc tccttggcga taacccatcc cgtcgggatt tgcggacatt tttctgccgg
     1981 gccgctaaac ccaaactgct gcaactcttc acggacatcc tcatctgcaa ggatgagggg
     2041 tgatgaaaaa cacgcacaga tccctgttgt aaagagacaa ctcgagatca tcagaaagat
     2101 aaagaccttt gtttcccaac gcatggattc tgttgaactg ttcatgtttt ccacctcttg
     2161 atatgaaatt agttacatgg tcattgtaaa actttgattc ttgcaggtca agcaagaggt
     2221 gacaaatgac atagaaaaaa ggatggcagt tctgtctgcc atcctttttt ctgttacggg
     2281 cgcgggccgc caggaccacc gccaccggga gcaccgggtc ctccggggcc gccaccgccg
     2341 gggagaccag ggccaccgcc accggggcgc ccaccgagtg agagttcgaa ttcaccgagg
     2401 cctttgcggg cgtggggcat ttctccgcgg gccaattgag tggtgatgtc gccgaagcgt
     2461 ccgctcaatt gagtgaaatc aagcgtcccg cctccgggca tgccagggcc gccgccattt
     2521 ttgccaagtt ccatgaaacc agcctgagcg ttcttgccaa gttgtgccag attcatcaat
     2581 tcatcgtggg agagtttgtt atcgcccatt gccaggttgg cggcatcaat gaaatcgaag
     2641 gcgctctgaa agactgcaag ttgatcggtg gggacatcat gcggctggat gctgctgatc
     2701 tcgtccacgc ggccctgctg gtctgctttg atcacttcga tcatatcctg cgcctgactc
     2761 ttcacggctt cggctttcac ctgcgcttcc tgggcggcat tttccaattg ggcaatgctt
     2821 tcttcagcca gtgcaagacc ttgttccagc gtggtgctga tctcctgtag tgactggtca
     2881 atggagacga gcgtttcgtt caattgattc aactctgctt cgatggcttg catttcggca
     2941 atggcctgat aggcaaggtc tgaatacagt tcgtaatatt gtgccatcag gtcttcggca
     3001 tattcgacgt agtaatcagc gtagtaatag taatcgtaca cataagtgac ttcttcagtg
     3061 gtgacggtgt cgtcgctggt ggtggtgtac acagaactgg tggtggcttc ggttgctgcg
     3121 accgcttcag cgacggcctc atcaatgagg gctgcaagtt cttcttcggt cagtgccaca
     3181 taatccacac ttgggcctgg ggtgggggtg gcgggcatgg cggtgagcac attggcgttg
     3241 actgtagcct gtgccaatgc atttgcctgc tgtgtccctg cgatggcggt gttgatgacg
     3301 atgttcgggt cttgcgcggc tggggtggcg gctgtgcctc cgcaggcgag tgtcactgcc
     3361 atgagcaaaa tgacgctgat gatgatttga agtttgtttt tcattggttc ctccaattga
     3421 tggttgtttt tcatcgggtt gctttttcgt gtgttgaatt tttttgtctt ttattcacta
     3481 tacatctctc ttttcgataa gtacctttac acctttgttg ggacgcaggg ttacgctggg
     3541 tttgggtgtg gcgtgtttgg cctgggtggg aaggatctgc cagtggcgcg agatgatggc
     3601 gaggatggca atggcttcgg tcatggcaaa gtgctcaccg atgcagaccc tgtttccgcc
     3661 gccgaatggg aagaaagcgt aacggggaaa tggaagcctt tcgatctcag accagcgttc
     3721 gggtcggaat tgaagcggcg agtcgtaaaa tcgcgggtca tgctgggtca cccattgact
     3781 catgagcacg accgatccag ctttgatatc ctgtccgccg atcgtacaat cttgcacggc
     3841 ctgcctgccc agagcccacg cgggcgggcg cattcgcaga gtctctttca cgaccatgcg
     3901 ggtgtagttg aggcttttga tatcttccga ggtggggaag cggtcgccga gcacggcatc
     3961 ggcttcggcc tgcagtttgc cctgcacgaa cggatcttgt gagatcatgt cccagcacca
     4021 ggagagcagc agtgcggtgg tttcatggcc tgccagcaaa aaggtgagta catgtgcatg
     4081 cagttgttcg tcggtgaatt ggtcgtggtg ttcgttcttc aaaatggtga tgagcgcgcg
     4141 cttggcttgt tccgagtgcg gattggcaag cagggtttcg gtgacaatgt tgaggtcggt
     4201 cttggcctgc ctgaatttgg ggaataacat gcgcgtcgcc tccggtaacg gaagctgggc
     4261 cagcggaaag aggcggacca gcgtcttcat cgctttgctc acgcgttcca ccgagccttc
     4321 gggtgcgctg ccgaaaaacg accacagggc aatgttcaaa gtcagccgca tcatttcatc
     4381 agacatatcc acgaccgagc cgtgctgcca gcgggtgagg tgctcaaaag tgttgagccc
     4441 catcacaggc aggtactctt ccatttttgt gcggtgaaag gcaggctgta aagcacggcg
     4501 ctgtgagaga tgttcatcgc cttcactggt gagcaaacct tcgcccagaa tgatgcgcgc
     4561 acgctgcagg ctgggcccct tgatgaaatt cgcctgctgc ttgaccagca cttcttcaat
     4621 gaattcagga tggttgagca gatagaaatc gcgttttggc attttcacgt gggcaatatc
     4681 gccattgtca cgcgcaaggt ctgccagcgt atgcagcgga ctgcgcaaca tgccccagat
     4741 aaaaccaaac ggtgtaatgt cggcatctac agatgaattg aggaaaaggt ttgtttccat
     4801 ggttcggctc cttgtttgtt gccccaagga tacaggaagc cgttttttat gtatgcccgc
     4861 tgtggagggt gattcctccc cctatttggt gggtctatct cattccccta ttttggtttt
     4921 gaaatgggga aagggatggt gtttacaatt cttcaggttt taccaaccca aaccgcaccg
     4981 ccatcaacgc ggcttgggtg cggtcgctca cgcccagttt catcaaaaca tcgctgacat
     5041 agcccttcac cgtcatttcg gtcagtgtca acttaacggc gatttccttg ttgctcatgc
     5101 cgcggccgag caatttcaaa atttcacgtt cgcgctcggt caattttccg aacgggtctt
     5161 cgggcatcgg cgcatgtgcc atcaggcgtt tggcaatatc ggggtgcagt tgcggtttgc
     5221 ctttggccgc cccgcgaatc gcttcgacca attcatctcc gcccatttcc tttagtagaa
     5281 atccgcttgc acccgcccgc aaggccgcat acacatgggc atcatcggtg aacgaggtca
     5341 ggatcaacac aggcagttcg gggaattcag ggcggatggc tttcaacgtt tccacgccat
     5401 ccagctctgg catttgcaaa tccatcagca ccacatcggg gctgtgtttt ttgatgagcg
     5461 ccaaaacctc cgtgccattt acggcttcgg ccagcacctt caaatctttt tcagcttcga
     5521 tcgtcatccg cagcccgcgg cggacgatct gatggtcatc ggcgatgatt attttcagca
     5581 tgggtttctc tccgttttcc tcgagcgtct gaaaactcag ccacgaaatt gcattcatgg
     5641 tgaaaagtat
//
LOCUS       DAOQJC010000191         5601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_8359_length_5601_cov_7.824919, whole genome shotgun sequence.
ACCESSION   DAOQJC010000191 DAOQJC010000000
VERSION     DAOQJC010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8359_length_5601_cov_7.824919"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3786
                     /locus_tag="PLE39_18235"
     CDS             <1..3786
                     /locus_tag="PLE39_18235"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE70329.1"
                     /translation="PIIGSDRVIGAIIVQSHEREYAFSEAQVRLLQTVAASMGVSLEN
                     ARLFDETQRLLKVTEDRAAELAIINSVQEGLASKLDMHAIYDLIGDKLGEILHTHDID
                     IRIFDPANNKVYFPYLKEKGTRIEIQSSEFRGMSKYVYDTKQMLVVNEDLPAFMKKVG
                     SNTLPGTQMEKSFVALPITSNGEVIGMVGISDYEKENAFSESELRLLQTVVSSMSVAL
                     ENARLFDETQRLLKETEERNAELAVINSVQAALSTEISVEGIYDAVGSKMREIFKAEI
                     GIRIYDPVTNLIHLPYLYEKGERVHPEPYPLTDKGFAAHVFRTRETLVVNENAAEVRE
                     KFGSYLLPGTASAKSSIYVPMIISGQVRGLVSISNYEREHAFSDSIVRLLQTLVNSMG
                     VALENARLFGETQRLLRETEQRAAELGAIGAVTQALVAETELDKLIQLIGSQTRDIFN
                     ADIAYLALYDSKAGNIHFPYQHGDTFPSIQLGEGMTSRIIQNGHPLLFNRNLDEQSVA
                     MGINRMGRKARSYLGVPIKAGRETIGVISVQSTQKEGAFNEDSLRLLTTIAANAGSAI
                     HTAQLHAETERRAREMATLTEVGRDISSTLDSSTVLERISQNAMELLNGELSALFLPE
                     NNGLIFRAIAAVGQNADELRNDTIELGAGILGAIAMNKVGEIVNDANNDPRALTIAGT
                     ENTPDEHMLAVPMLANDELKGLMAVWRTGAERQFTEAELEFLNNLSRQAVIAVQNSQL
                     FAEAQEARAAAENANQAKSAFLATMSHELRTPLNAIIGFSRIVRKKAEGVLPEKQTEN
                     LDKVLSSAEHLLGLINTVLDIAKIEAGRMDVQAANFNINALVDQCYNTAQPLVKANVE
                     FKKSNDTNLPLIFSDQDKIKQIILNLLSNAAKFTHKGSVTISTRYAESNFMVDVTDTG
                     IGMSEEALGRIFEEFQQADSSTTRQYGGTGLGLAISRNLARLLGGDLTVTSEQGKGSV
                     FTLSLPIQYQSEVSPASSSDPQTGSTQKTEPRLKVTSTKKRVLVIDDDPDAVYLLQET
                     LNTNEFEVVGARSGMDGHRLARQLMPDAILLDILIPDKDGWQVLHDLKVDERTANIPV
                     ILLTIVDKKALGFRLGASAYLLKPLNPREVIDTLSRVTKKIGRTHIHVLVVDDDPHIS
                     DMLKQILPASEFDLRSAEDGVQGLEAIAMSVPDVLLLDIMMPRLDGFGVIKRLRANPA
                     TRELPIIVISAKELTDDEAARLKESVAFIMRKQGFDGEKLVEEIRSVAHLPPGVKSS"
     gene            3827..5008
                     /locus_tag="PLE39_18240"
     CDS             3827..5008
                     /locus_tag="PLE39_18240"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE70330.1"
                     /translation="MNNQPKILIVDDEPFNVDYLEQELEDLNYTTVTAVNGQEALEKI
                     SSEAPDLVLLDIMMPIMDGFSVLGKVKADPAIRDIPIIVISANNDLQSTVKGIQLGAE
                     DYLPKPFEPTLLKARIQSSLEKKRLRDMQSLYLKSLEREMNIARDIQKEFLPAQLPEV
                     PGWEIASYFEAAKEVAGDFYDAFTLPDGTLTFLVADVCGKGIGAALFMTLFRSLIHAA
                     SISDQFSVGQAQPSLSAAERLQHAVTLTNNYVAETHEASNMFATVFVGILDPATGKLT
                     YINAGNEPPLIAGGDGKVHTTLARTGSAVGAIANAKFTVKETTLASDDMLVAFTDGIP
                     DTQNIHGEFFGNDRMRGLLVDNSTPTALLKAMETDLDQFIGEADQFDDITLLAIKRKA
                     S"
     gene            5138..5542
                     /locus_tag="PLE39_18245"
     CDS             5138..5542
                     /locus_tag="PLE39_18245"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE70331.1"
                     /translation="MEIKVTTQNSRVPVTIIHIDGNLDSSSYTKFQKTAEELIQNGSR
                     HILVDLEHSPYVSSAGFRVLSQIFKELNSIHPDANLSESEMKEGIINGSYKSPYLKLA
                     NLSADTKAVFVTTGFDMYIEEYDDLKAAVASF"
BASE COUNT         1467 a         1701 c         1353 g         1080 t
ORIGIN      
        1 cccatcatcg gcagcgaccg tgtgatcggt gccatcatcg tacaaagtca cgaaagggaa
       61 tacgccttca gcgaggcgca agtgcgcctg ttgcaaactg tggcggcaag catgggtgtg
      121 tcgctcgaaa atgcgcgcct cttcgatgag acccagcgcc tgctcaaggt gacagaagac
      181 cgtgccgctg aactcgccat catcaactcg gtgcaggaag ggctcgcctc caagctggat
      241 atgcacgcca tctacgacct catcggtgac aaactgggcg agatcctgca cacacacgat
      301 attgacatcc gtatttttga cccagccaat aacaaagtgt acttccccta tctgaaggaa
      361 aaaggaacgc ggatcgagat ccaatcgtca gaattcaggg gaatgtcgaa atatgtgtac
      421 gacacaaagc agatgctggt ggtcaatgaa gacctgcctg ctttcatgaa aaaggtcggt
      481 tcaaacaccc tgcctggcac acaaatggaa aaaagttttg tagccctgcc catcaccagc
      541 aatggagaag tgatcggcat ggtaggcatc agcgattacg aaaaggaaaa cgccttcagc
      601 gaatcagagc tgcgcctatt gcagacggtg gtttcttcca tgagcgttgc cctcgaaaac
      661 gcccgccttt tcgacgagac ccagcgcctg cttaaagaga ccgaggaacg caacgctgaa
      721 ttggcggtca ttaacagtgt gcaggcggcc ctctccaccg agatcagcgt ggaaggcatc
      781 tatgatgcgg tcggaagcaa gatgcgcgag atcttcaaag ccgagatcgg aatccgtatc
      841 tacgaccctg tcacaaacct gatccacctg ccctacttgt atgaaaaggg tgaacgcgtc
      901 caccccgaac cctatccgct caccgacaag ggatttgccg cccatgtttt ccgtacccgc
      961 gaaaccctgg tggtgaacga aaacgcggca gaggtacgcg aaaaattcgg ttcttatctt
     1021 ctgccaggta cagcctcagc caagtcgtcc atttacgtgc cgatgatcat cagcggacag
     1081 gtccgcgggc tggtttccat cagcaactac gagcgcgaac acgcctttag cgactcgatc
     1141 gttcgactcc tgcaaacatt ggtcaattcc atgggcgtgg cgcttgaaaa cgcccgtctc
     1201 ttcggcgaga cccagcgcct gctgcgtgaa accgaacaac gcgccgccga attgggcgcc
     1261 atcggtgcag tcactcaagc cctcgtggca gagactgaac tcgataaact gatccagctt
     1321 atcgggagtc agacccgcga tattttcaat gcagatattg cctatctggc cttatacgat
     1381 tccaaggcgg gcaacatcca cttcccgtat cagcatggcg ataccttccc atcgattcaa
     1441 ctcggcgaag gcatgaccag ccgcatcatc caaaatggac atcccctgct cttcaaccgc
     1501 aatctggatg agcaaagtgt ggcgatgggc atcaaccgca tgggcagaaa ggccaggtcg
     1561 tatctgggcg tgcccatcaa ggctggccgc gaaaccatcg gcgtgatcag tgtgcaaagc
     1621 acacaaaagg aaggcgcctt caacgaagat tcgctgcggc tgctcaccac cattgccgcc
     1681 aatgcaggct cggccattca taccgcgcaa ctgcatgctg aaacggaacg ccgcgcccgc
     1741 gaaatggcca ccctcaccga agtgggccgc gatatttcct caacccttga ttcctccacc
     1801 gtgctggagc gtatctccca aaatgcaatg gagctgctca acggtgaact cagcgcgctc
     1861 ttccttcccg aaaacaacgg cctgatcttc cgtgccattg cggctgtggg gcaaaacgcc
     1921 gacgagctcc gcaacgacac catcgaactc ggagcaggca tccttggcgc catcgccatg
     1981 aataaggtcg gcgaaatcgt caacgacgcc aataacgacc cgcgtgcgct caccatcgca
     2041 ggcactgaaa atactcccga cgaacatatg ctggcagttc ccatgctcgc caatgatgaa
     2101 ttgaaaggcc tcatggcggt ctggcgcaca ggcgcagaac gtcaatttac agaagccgaa
     2161 cttgaattcc tcaacaacct ttcacgtcag gccgtgatcg ctgtgcaaaa ttcacaactc
     2221 tttgctgaag cgcaggaagc ccgtgcagcg gctgaaaacg ccaatcaggc caagagcgcc
     2281 ttccttgcca ccatgagcca cgagctacgc accccgctca acgccatcat cggtttcagc
     2341 cgcatcgtcc gcaagaaggc ggaaggtgtg ctgcccgaaa aacagaccga gaatctcgac
     2401 aaagtccttt ccagcgccga acacctgctc ggcctcatca acaccgtgct cgatattgcc
     2461 aagatcgaag caggccgcat ggatgtgcag gccgccaact tcaacatcaa cgcgctggtc
     2521 gatcaatgtt acaacaccgc ccaaccactg gtcaaagcaa acgtcgaatt caagaagagc
     2581 aacgacacca atttgccgct catcttctcc gatcaggaca agatcaagca gatcatcctc
     2641 aacctgttga gcaacgccgc caaattcaca cacaaaggca gtgtcaccat cagcacgcgg
     2701 tatgctgaat caaacttcat ggtggatgtg accgacacag gcatcggcat gagcgaagaa
     2761 gcacttggcc gcatcttcga agaatttcaa caagccgatt ccagcaccac ccgccaatac
     2821 ggcggcacag ggcttggcct tgccatcagc cgaaatctgg cgcgcctgct cggcggcgac
     2881 ctgaccgtga ccagtgaaca gggcaagggt tctgtcttca ccttgagcct gcccatacaa
     2941 tatcaaagcg aagtttcacc cgcttcttca tcggacccgc aaactggttc cacacaaaag
     3001 acagaaccgc ggctgaaagt gacctctact aaaaaacgcg tcctggtcat tgatgatgac
     3061 cccgatgccg tgtacctctt gcaggaaacc ctcaacacga acgaattcga agtcgtcggt
     3121 gcccgcagcg gcatggacgg tcaccgcctg gcgcgtcaac tcatgcccga tgccatcctg
     3181 ctcgacattc tcattccaga caaggacggc tggcaggtgc tgcacgacct gaaagtggat
     3241 gaacgaaccg caaacatccc cgtgatcctt ttgaccattg tggacaaaaa agcgctcgga
     3301 ttccgcctcg gcgcctcggc ctacctgctc aaacccctga acccgcgcga agtgatcgac
     3361 accctgagcc gcgtcaccaa aaagatcggg cgcacacaca tccacgtgct ggttgtggat
     3421 gatgacccgc acatctcaga catgctcaaa caaattctgc cagcctccga attcgacctg
     3481 cgctctgcag aagatggtgt gcagggactg gaagccattg ccatgtcggt gcctgatgta
     3541 ctcctgctgg atatcatgat gccgcgcctc gatgggttcg gggtcatcaa acgcctgcgc
     3601 gccaaccccg ccacacgcga actgcccatt atcgtcatca gcgccaagga actgaccgat
     3661 gacgaagccg cccgcttgaa ggaaagcgtc gcattcatca tgcgcaagca gggttttgac
     3721 ggtgaaaagc tggtggaaga gatcagaagc gttgcccatc ttccgcctgg agtaaaatcg
     3781 tcctgatctt tatcgaagcc gcattgattt caacctacgg agccgcatga acaaccaacc
     3841 taaaattctg atcgtggatg acgaaccctt caacgtggac tatctcgaac aggaactcga
     3901 agacctgaat tacaccaccg tcaccgcggt caacgggcag gaagccctcg aaaagatcag
     3961 cagcgaagcc cccgacctgg ttttgctcga tatcatgatg cccatcatgg atggtttcag
     4021 cgtgcttgga aaggtcaaag ccgaccccgc cattcgcgat atccccatca tcgtcatttc
     4081 agccaacaac gacctgcaaa gcaccgtgaa gggaatccaa ctcggcgcgg aagactatct
     4141 ccccaaaccg ttcgagccga ccctgttgaa ggcacgcatc caatcgagcc ttgaaaagaa
     4201 gcgccttcgc gatatgcaaa gcctctacct caagagcctc gaacgtgaaa tgaacatcgc
     4261 acgcgacatt caaaaagaat tcctgcctgc gcaattgccc gaggtgccag gctgggagat
     4321 cgcttcgtat ttcgaagccg ccaaggaagt ggctggcgat ttctacgatg ccttcaccct
     4381 gcccgatggc accctgacct tcctcgtagc cgatgtatgc ggaaaaggaa tcggcgcggc
     4441 attgttcatg accctcttcc gcagcctcat ccacgcggct tccatttccg atcaattctc
     4501 tgtagggcag gcacagccat ccctttctgc ggctgagcgg ttacagcacg ccgtcacgtt
     4561 gaccaacaac tacgtggccg aaacccacga agcatccaac atgttcgcca cggttttcgt
     4621 cggcatcctc gaccccgcca ccggaaagtt gacctacatc aacgccggca atgaaccgcc
     4681 gctcatcgca ggcggagacg gcaaggtcca caccaccctc gcgcggacag gttccgccgt
     4741 tggcgcgatc gcaaacgcca aattcaccgt caaagaaacc accttagcca gcgacgacat
     4801 gcttgtcgct ttcaccgacg gcatccccga tacacaaaac atccacggcg aattcttcgg
     4861 caacgaccgt atgcgcggcc tgcttgtgga caactccaca cccaccgccc tgctgaaagc
     4921 catggaaacc gacctcgatc agttcatcgg cgaagcagac caattcgacg acatcacctt
     4981 gctggcgatc aaaagaaaag cctcgtaaac cccaaatgcc aacgagaaag ccaggcatct
     5041 tgaaaaacgg aataccgccc taacgggcct gattttttgc ctttaacaat gagccatcaa
     5101 aaaatcattt caatcaaatc attttaagga gagtatcatg gagattaagg taacaaccca
     5161 aaacagccgc gtaccagtga cgatcatcca cattgacggt aaccttgatt cgtcatccta
     5221 caccaagttt caaaaaacgg ctgaagagct gatccaaaac ggttcacgcc atatccttgt
     5281 cgatctggag cactcgccct atgtaagcag cgcgggtttc cgcgttctta gccagatctt
     5341 caaggaatta aattccattc atccagatgc caacctgagt gaaagcgaaa tgaaggaagg
     5401 catcatcaac ggctcctaca aatctcccta cctgaaactg gcaaatcttt cagccgacac
     5461 aaaggctgtt tttgtcacca cagggttcga tatgtacatc gaagaatacg acgacctgaa
     5521 agcggcggtc gcatctttct aggcacggcc cattcaaaaa ggaaatcata aaaaactcgg
     5581 aacaggtggt ttcgagtttt t
//
LOCUS       DAOQJC010000192         5596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_8367_length_5596_cov_8.515972, whole genome shotgun sequence.
ACCESSION   DAOQJC010000192 DAOQJC010000000
VERSION     DAOQJC010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8367_length_5596_cov_8.515972"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLE39_18250"
     CDS             <1..476
                     /locus_tag="PLE39_18250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE70332.1"
                     /translation="NTFLNALTHLPDFEGRSSVSTWLYRIASNEALMMLRKKRPLNIE
                     DLQAENSDEGMVPEIFVDWSALPEGELLAGESKNVIDDAVAKLPEQLRIVFVLRDIEG
                     ISIRETAEILSLTETNVKTRLLRARMALREKLSAYYGEQRQTHASKGKNEPSSPR"
     gene            454..717
                     /locus_tag="PLE39_18255"
     CDS             454..717
                     /locus_tag="PLE39_18255"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNE70333.1"
                     /translation="MNHPLHDNCKALLGSLSEYIDGELPSDLCREIEKHLEGCDNCRV
                     VLNTTKRTIDLVQIPVKEESVPDDVRERLFKRLKLDDYLNPQP"
     gene            complement(734..2335)
                     /locus_tag="PLE39_18260"
     CDS             complement(734..2335)
                     /locus_tag="PLE39_18260"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNE70334.1"
                     /translation="MDKPWYHKTSIYQIYPRSFHDTNGDGIGDLKGIIQKLDYLHELG
                     FETLWISPFFSSPQADTGYDISSYTDIAPEYGSMEDALQLIEGVHQRGMKIVFDMVMN
                     HTSDEHPWFRESRSSQDNPKANWYIWQDKPNNWQSMTGGSGWHYAPERNQYFWSSFLP
                     FQPDLNYRNPEVKRAMLEIVRFWLGKGVDGFRLDIFNVIYKDAAFRDNPFTFKLMPTE
                     DDPSGFFQEAKYNLNQAESFEFARELRSVCEEFGERMLLGEVSGNKSTLRKFLGEETN
                     NGLGLVFDFEMLSFQFTAEYFRELLERIEAHFPAPFMPVYVFSNHDRRRSILRLGNDL
                     RKAKLLAMLQLTTRGVPCMYYGEEIGMTDLPQPFGAARDPIPHKFKLIPRFIFNWLNL
                     TINRDEVRTPMQWDGTKNAGFSSSEQTWLPVHKNHLEINVEKANQDESSLLNIIRKLM
                     KIRKNETVLQEGSLEVLKNLPHGILGYRRTWDHTSLTILLNFDGSEKVVRTEFSECIF
                     ALSARADLKEKAIHMNGFGGVILRA"
     gene            complement(2340..3629)
                     /locus_tag="PLE39_18265"
     CDS             complement(2340..3629)
                     /locus_tag="PLE39_18265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE70335.1"
                     /translation="MIQNTCLWWETAPTPASYKNKPLPAKTDVVVVGGGFTGTSAALQ
                     LAKGGARVTLLEAKTIGFEASSRNGGQALSCLHQTLTNAIKEHGRELAKNMFLAATYA
                     ANTVEQIVTEEGLDCDFVRCGNIEAAFKPAHFEKLKREQETLHEVAGYEVQVLPRNET
                     RTELGTDSYNGMMINPCSGSVQPAKFVRGMAMAAERAGADIHEGTRVIVIERISDSPA
                     SDGSRFLVKTERGDILTKEIMLAANAWIGDILPQFRRRVLPVESYIIATEPLSEDLAK
                     KLIPNNRVVYDTRKTLAYYRLSPDNRMVWGGELTFANTSQRTNIHALQRGMNKVFPEL
                     ANHKIDYFWNGTMGITFDENAHAGQIDGMWYSMCYVGHGVTLGTYLGRQMANGILGKP
                     SNNPFADLPIPYASPFQENSWVVDAGKLWFRFLDIFQ"
     gene            3927..5528
                     /locus_tag="PLE39_18270"
     CDS             3927..5528
                     /locus_tag="PLE39_18270"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HNE70336.1"
                     /translation="MKPLRKAIGFSVLLLLLFTSACGGSAPAATEAPAATEAPASPDY
                     SFDAPAATEAPAAEAPAAIPQQQNGQQPPATGNDPYDMFFEDYGVNPSIDTEDDNLST
                     FALDVDTGSYTVMRNYLQDGNLPPQDSVRVEEYVNYFDHGYPTPPPHQAFGIYVDGGP
                     SPFTETERYDMLRIGIQGYEVADEDRKDAALTFVIDVSGSMDMDNRLGLVKHSLELLV
                     EQLHRNDTVSIVVYGSEARVVLEPTRGSDSGRILDAIYSLSPEGSTNAEAGLRLGYQM
                     AMEAFMRDGINRVILCSDGVANVGLTGPDAILGEINHYVEEGVTLTTIGFGMDNYNDT
                     LMEQLADNGDGFYAYVDDIDEARKLFIDQITGTLQTIALDAKVQVDFNPQVVMRYRLV
                     GYENRAVADDEFRDNTVDAGEIGAGHSVTALYEVKLYPEARGRIATVFMRWEDPDTHQ
                     VVEISKDFETSQMAYDFRETDPYFQRAVVVAEFAEILKGSYWAEESSLDDVYDEARRI
                     ADYLYRDDVMDEFVDLVREARRLMD"
BASE COUNT         1290 a         1377 c         1481 g         1448 t
ORIGIN      
        1 agaacacatt tttgaatgcg ctgacacatc tccctgattt tgaaggtcgt tcaagcgttt
       61 cgacatggct gtatcgcatt gcttcaaatg aagccctgat gatgctccgt aagaaacggc
      121 cgctcaacat tgaagacctg caggcagaaa actctgatga aggaatggtg ccagagattt
      181 ttgtggattg gagcgccctg cccgaaggcg agctgctcgc gggtgaaagc aaaaacgtga
      241 tcgatgacgc ggttgcaaaa ttacccgagc aattaagaat tgtctttgtc ttgcgcgaca
      301 tcgaagggat ttcgatcaga gaaactgcag agatcctctc gctgacagag accaatgtaa
      361 aaacaagatt attgcgcgcc cgcatggccc tccgtgaaaa attgtcggcg tactatggcg
      421 aacaacggca aacacatgcc tcgaaaggaa aaaatgaacc atcctctcca cgataactgc
      481 aaggccctgc tgggctcgct ctccgaatac attgacggtg aattgccgtc tgatctgtgc
      541 agggagatcg aaaagcatct cgaaggctgc gataactgcc gtgtcgtttt aaataccacc
      601 aagcggacga tcgaccttgt gcagattcct gtaaaagagg aatctgtccc cgacgatgtg
      661 cgcgaacgcc ttttcaaacg cctcaaactg gacgactacc tgaatccaca gccctaggca
      721 ttttcttgcc ttgtcaggct ctcaaaatga ccccgccaaa gccattcatg tgaatggctt
      781 tttcttttaa atccgctctg gctgacaggg caaagataca ctccgaaaat tctgtgcgga
      841 ccactttttc gctgccatca aaattcagca ggatggtcag gcttgtatgg tcccaggttc
      901 gtctgtaccc cagaattcca tggggtaggt ttttgagtac ttccagtgag ccttcctgta
      961 aaactgtttc atttttccga attttcatca gctttcggat gatgttcaat aaagaagact
     1021 catcttgatt tgccttttcc acgtttattt caaggtggtt tttgtgtact ggcagccagg
     1081 tctgttctga agaagaaaac cccgcattct ttgtcccatc ccactgcatc ggggtgcgga
     1141 cttcatcacg gttgatggtc aggttgagcc aattgaagat gaagcgcgga atgagtttga
     1201 atttatgcgg aattgggtcg cgggcagcgc cgaagggttg cggcaggtcg gtcatgccga
     1261 tctcttcgcc gtagtacatg cacggcacgc cgcgcgtggt gagttgaagc atggcgagca
     1321 gttttgcctt gcgcaggtcg ttgcccagcc tgagaatact gcgccgcctg tcgtgattgc
     1381 tgaagacata cacaggcatg aacggagcgg ggaaatgtgc ttcgatgcgc tcgagcaatt
     1441 ccctgaaata ctctgctgta aattggaagc taagcatttc aaaatcgaag accagcccca
     1501 gtccattgtt ggtttcttcg ccgagaaatt tgcggagcgt ggatttgttt ccgctgacct
     1561 ccccgagtaa catccgctcg ccgaattcct cgcacacact gcggagttcc ctggcgaatt
     1621 cgaaactttc ggcttgattc aggttgtatt ttgcttcctg aaaaaatcca gatggatcat
     1681 cttccgtggg catgagtttg aatgtgaaag ggttgtcgcg aaatgcggcg tccttgtaaa
     1741 tcacattgaa aatatccagc cgaaagccat ccacgccttt gcccagccaa aagcggacaa
     1801 tttccagcat ggctcgtttg acttccgggt tacggtagtt caggtctggt tggaaaggta
     1861 aaaaactgga ccagaaatat tgattgcgtt caggggcata atgccagccg cttccgcccg
     1921 tcatgctttg ccagttgttg ggcttgtcct gccagatgta ccaattggcc ttggggttat
     1981 cctgtgaaga gcgcgattcc ctgaaccatg ggtgctcgtc tgaagtatgg ttcatgacca
     2041 tgtcgaagac gattttcatg ccgcgctggt gaacaccttc gatgagttgc agggcatcct
     2101 ccatgctgcc gtactcggga gcaatgtcgg tgtagctgct gatatcgtag ccggtatccg
     2161 cttgcggtga ggaaaagaac ggcgagatcc aaagggtctc gaatcccagt tcgtgcagat
     2221 aatccagttt ttggatgatg ccctttaggt cgccgatgcc atcgccgttc gtgtcgtgga
     2281 atgaacgcgg gtagatttga tagatgcttg ttttgtgata ccagggtttg tccatgtcgt
     2341 tattgaaaga tatctaaaaa gcggaaccag agtttgcccg catcaaccac ccaggaattt
     2401 tcttgaaatg gtgaagcgta gggaatgggt agatcggcaa atggattgtt gctgggtttt
     2461 ccgagaatgc catttgccat ttgccgccca agatatgtgc caagtgtgac gccatgccca
     2521 acgtagcaca tcgaatacca catgccgtca atttgacctg catgggcgtt ttcgtcgaac
     2581 gtaattccca ttgtcccatt ccagaaataa tcaattttgt ggttcgcaag ttcgggaaag
     2641 accttgttca ttccgcgctg caaggcgtgg atattcgttc gctggctggt gttggcaaaa
     2701 gtaagttcgc cgccccatac catgcggtta tcaggcgaga gacgatagta agcgagcgtt
     2761 tttcgtgtgt cgtatacgac gcggttgttt gggatgagtt tttttgcaag atcttcagat
     2821 agaggctcgg tggcaatgat gtaactctct acaggaagca cgcgccgacg gaattgcggc
     2881 aggatgtccc cgatccatgc gttggcggca agcatgatct cttttgtgag gatgtcgcct
     2941 ctttcagttt tgacaaggaa tcggctgccg tcgctggcag gcgagtcgct tatccgttcg
     3001 atcacgatta cgcgcgtacc ttcgtgaata tccgctcctg cacgttctgc cgccattgcc
     3061 atgccacgca cgaatttggc aggctgcacc gatccgctgc atggattgat catcataccg
     3121 ttgtatgaat ctgtgcctag ttcggttcgt gtttcatttc tgggaaggac ctgaacctca
     3181 taaccagcca cctcgtgcag ggtttcctgc tcacgcttca atttttcgaa atgggctggc
     3241 ttgaaagcgg cttcaatatt cccgcagcgg acgaagtcac aatccagccc ttcttcggtg
     3301 acaatctgtt ccactgtgtt ggcggcatat gtggcggcga ggaacatatt tttcgcgagt
     3361 tcccgtccat gctcttttat ggcgttggtc aaggtctggt gcaggcagga aagcgcctgt
     3421 ccgccgttgc gtgagctggc ttcaaagccg atcgtttttg cttcgagcaa ggtcacacgc
     3481 gctccgcctt tcgccagttg cagtgcagcc gatgtgcctg taaagcctcc gccgaccacg
     3541 accacatccg ttttggcggg caatggttta tttttgtaag acgctggcgt tggggcggtc
     3601 tcccaccata agcaggtatt ttgaatcatg ctggtttcct cgttgcaggg ttgatgtgaa
     3661 cagattgtat atcaccactt atcaaaacta caagttgatt tttgtgtctg ggtgtgccac
     3721 aatgactttg acgttttgaa tgggggaact ttccccgcag gcaatacgtc caacccatat
     3781 cacaaacctg gcgaataagt gggacagcct atattagaaa ggagggccta tgacctgata
     3841 aaaacttttg aagctcagcg ggacgccgcc tgctgacctg atttactgtt tgtttctgaa
     3901 atataaaaaa taacaggaga agaaaaatga aacccttacg caaagccatc ggctttagtg
     3961 ttctattatt gctgttgttt acctccgctt gtggaggctc tgcgcccgcc gcgaccgaag
     4021 cacccgcagc aacagaagcc ccggcgtcac cagattattc ttttgatgcc cctgctgcaa
     4081 ccgaagcacc cgccgcagaa gcgcctgccg ccatcccgca gcaacagaac gggcaacaac
     4141 cccctgcaac tggcaacgat ccatatgaca tgttcttcga agactatggc gtcaacccat
     4201 ccatagatac cgaagacgat aacctttcca catttgcttt ggatgtggat acgggctcgt
     4261 acactgtcat gcgtaattat ttgcaagatg gcaaccttcc accccaggat tctgtgcgtg
     4321 tggaggaata cgtcaattat ttcgatcacg gatatcccac cccgccgccg catcaggctt
     4381 ttggcattta tgtggatggc ggcccttccc ccttcactga aaccgaacgc tacgatatgc
     4441 tgcgcattgg cattcagggt tacgaagtag ccgatgaaga ccgcaaagac gcggcattga
     4501 ctttcgtgat tgatgtttca ggttcgatgg atatggataa tcgcctcggt ctggtcaaac
     4561 attcgctgga actgctcgtt gagcaattgc atcgaaatga caccgtcagc atcgtggtct
     4621 atggatcaga agcccgcgtc gttcttgagc cgacccgtgg ttctgattct ggaagaattc
     4681 tcgatgcgat ttacagcctg agccctgaag gctccaccaa tgcggaggcg ggtctgcgcc
     4741 ttggctatca aatggcgatg gaggctttca tgcgggatgg gatcaatcgg gtgatcctgt
     4801 gctccgatgg cgtggccaat gtcgggttga caggccccga tgccatcctt ggggagatca
     4861 atcactacgt ggaggagggc gtcaccctca ccaccatcgg ctttggcatg gacaattaca
     4921 acgataccct catggagcag cttgccgata acggtgatgg cttctacgcc tatgtggatg
     4981 acattgatga agcccgtaag ttgttcattg accagatcac tggcaccctg caaaccatcg
     5041 cgttggacgc aaaagtgcag gtggatttca atccgcaggt ggtgatgcgt taccgcctgg
     5101 ttgggtacga aaaccgcgct gtagccgatg acgaattccg tgataatacg gtggacgcag
     5161 gggaaattgg cgctgggcat tcggtgacgg ctttgtatga ggtcaagttg tatcctgagg
     5221 cacgcggcag gatcgccact gtgtttatgc gttgggaaga ccccgataca catcaggtcg
     5281 tcgaaatttc aaaggatttc gagaccagtc agatggcgta cgatttccgc gagacagacc
     5341 cgtacttcca gcgtgctgtg gttgtggcag agttcgctga aatcttgaag ggcagctatt
     5401 gggctgagga aagctcgctt gatgatgtgt acgatgaagc ccgccgcatc gccgattatc
     5461 tctaccgtga tgatgtgatg gatgaatttg tggatctggt ccgtgaggca aggcggttga
     5521 tggattaaga ggctcacgcc tttcaacacc gataaaaaat aagtggcggt tgttttaacc
     5581 gccacttttt tttttt
//
LOCUS       DAOQJC010000193         5586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_8393_length_5586_cov_10.068884, whole genome shotgun sequence.
ACCESSION   DAOQJC010000193 DAOQJC010000000
VERSION     DAOQJC010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8393_length_5586_cov_10.068884"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1906)
                     /locus_tag="PLE39_18275"
     CDS             complement(<1..1906)
                     /locus_tag="PLE39_18275"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE70337.1"
                     /translation="MRELPFGTVTFLFTDIEGSTKLWEQHPEAMKTALAKHDSILKDA
                     IESNRGHVIKTTGDGFHAVFATAMDGINASVTAQRELHSSFFLHPSSLSLKVRMGIHT
                     GEAELRNNDYYGGTLNRAARLMSVANGGQVLLSNTTADLVREQLPAEITLRDLSEHRL
                     KDLTRPERIFQIVHPELPSEFPPIKTLDAFPNNLPAQLTSFIGREKEINEIINALNTS
                     RLVTLTGSGGTGKTRLTQEVGVQLLTNFQHGVWLVELAPLSNEAQIIPALAQAFGLQE
                     LPFNPLAKLVDDYLRDKKLLLILDNCEHLIDACARLADHLLHLSAGVKILASSREALG
                     IAGEVAYRTPSLADSESTSLFLDRARAANSKFSLTESNASAIAQICHRLDGIPLAIEL
                     AAARTKILSAEQIATRLDDMFRLLVGGSRTALPRQQTLRALIDWSYDLLSDEEKRLLQ
                     FASVFVGGWTLDALEFVANEPNTLELLEQLVNKSLVVTEEREGAMRYFLLETIRQYAR
                     EKLFEAKQASAARDRHFIHYKNSTSGLWDVSRFNNETELQRLKAMHGEMENLRAAFEW
                     GLQNHVQDALDLAANMATGITMIGGPMEGTAMLKMAMEKFRALPPAAGADADANRVRK
                     VIYAHGCFGLG"
     gene            2038..5103
                     /locus_tag="PLE39_18280"
     CDS             2038..5103
                     /locus_tag="PLE39_18280"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE70338.1"
                     /translation="MDRILETFKIKPGEERMSALLMGLMLITAAGGAIGGNATEALFF
                     ARFGVEFLPTMYVILGVFTFLTSMAITIVMGRFSKQRLFTTLPFFIGLILIVEWIVLR
                     FDIRWFFAAMWLGMNVINSLQGLAIWGLAGTACDTRQAKRLFPLFSAGGILGAVLGGL
                     LTQSLARWLHSENLLLVWAGTLFIAFLLGRALIGRSVGPNITSARIKQPGIFVEMQRG
                     YNFVRRSPIMQWVSYSAILFSVCYFSLALPFSRGATAQFTNADRLAGFLGLFQSITTI
                     VALIVSLFFANRLFARFGIMPMLLLFPIIYFVGFGVLSFSAPFAILVSVRFMQMMWMQ
                     GIAGTAWQALFNVVPSEQRDQVRTFIGGVPEQAGTFIAGLILVVGEQTLAPGQLYFIG
                     LIAAAFLIYMLWRASRDYSIALVEALRAGQPQMFVSEEEPFGGFSTDATAVAAALKGM
                     SDPDRLTRLVSIEILGHLSTPKVIAGMVSALTDEDAMIRSSALRGLTHARASSALLEI
                     AASLSDPEPEVRLQAVDSLRQLAQHPRGVSAHIEPLLTDQDPLVRTRSAQTLLSFGEH
                     PKAKSILHEMAAGTDITSRTHALNALAECRDESAFELGLSALDDAAPIIRKTAPRLLV
                     SIDPQRALEPILHHLNDDDSHVRKALAEAIGNIGLPALDSLLAALTNPSYEDGALTAL
                     ELLPAQKASQQIRVHAMNTARSALRYHQLALGLAQKFGRTISGDGRYQLLIESLHDKA
                     HRSGHNSLRAVGLLKDRQTIGIAIENLRSRESAQRANALEVLESIGEVEIIRPLLALW
                     ESGESSSASLPDQWLMELLHDPYSWLRACAVLAAAESDDEKIVLTLGSLSRSDPDATV
                     RDIANKVISGGHSMDTLATLPLMERILFLRRVPIFADLSPADLKQVAAITTEAVFADG
                     DILAHQGEFGDEMFIVVSGEVNVLIASDDQKESRYIAKRSTGDYVGEMSIISREPRIA
                     TLVASGQVRALCVGRKQFEGILRERPEIGMALMRTLCQRLKEASLQQTN"
     gene            complement(5174..>5586)
                     /locus_tag="PLE39_18285"
     CDS             complement(5174..>5586)
                     /locus_tag="PLE39_18285"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012897.2"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNE70339.1"
                     /translation="YQAEGVPSIVLAGKEYGTGSSRDWAAKGPMLQGVKAVIAESFER
                     IHRSNLVGMGVLPLRFAEGQNAESLGLDGSEVFDIEGLDNNIKPKAELMVKAKKADGK
                     VIEFKATALLNTDVEVNYYRNGGILHTVLRNLVK"
BASE COUNT         1235 a         1560 c         1419 g         1372 t
ORIGIN      
        1 tccccaagcc aaagcatcca tgggcataga tcacttttcg aacgcggttc gcatctgcgt
       61 cagcacctgc ggcaggcggc agggcgcgaa acttctccat tgccattttg agcatggcgg
      121 tcccttccat cggaccgccg atcatggtga tgcctgtcgc catgtttgcc gccagatcca
      181 atgcgtcttg cacatggttt tgcagtcccc attcaaacgc cgcacgcaaa ttttccattt
      241 cgccgtgcat agctttcaac ctttgcaatt cagtctcgtt attgaagcgc gagacatccc
      301 ataagccact cgtgctattt ttatagtgga tgaagtgccg atcacgcgcc gccgacgcct
      361 gcttcgcttc gaacagcttt tcccgcgcat attgccgaat cgtctccaac aggaaataac
      421 gcatcgcacc ttcgcgctct tccgtcacaa ccagcgactt gttgactaac tgctccaata
      481 actccagcgt attcggttca ttcgcgacga attcgagcgc atccagcgtc catccgccga
      541 caaacaccga cgcaaattgt aaaagtcgtt tctcttcatc ggagagtaaa tcgtagctcc
      601 agtcgatcaa tgcacgcaaa gtctgttgac gtggcaacgc ggtgcggctt ccacccacaa
      661 gcaagcggaa catatcatcc agccgtgtcg cgatttgttc tgcagagaga atcttcgtcc
      721 gcgcggcggc gagctcaatc gccaggggaa tgccatcgag acggtggcag atttgggcga
      781 tggcagaagc gtttgattcg gtcagggaaa attttgagtt ggcggcacgg gcacggtcca
      841 agaaaaggct ggtcgattcg gaatccgcaa gcgaaggagt gcgatatgcc acttcccccg
      901 caatgcccaa cgcttcgcga ctgctcgcaa ggattttgac tcccgcacta agatgcagca
      961 aatgatcggc cagccgcgcg caggcgtcaa tgagatgctc gcagttatcg agaatcagca
     1021 ggagcttttt atcgcgcagg taatcatcca ccagcttcgc cagcggattg aacggcaact
     1081 cctgcaaacc aaaggcctgc gccaacgcgg gaatgatctg cgcctcattg ctgagcggag
     1141 ccagttccac gagccacacg ccatgctgaa aattggtcag cagttgcacg ccgacctctt
     1201 gtgtgaggcg cgtcttgccc gtgccgcccg agccagtgag agtgaccaga cgtgatgtat
     1261 taagcgcgtt gatgatttcg ttgatctctt tttcgcgccc aataaacgaa gtgagttgag
     1321 cggggagatt attcggaaac gcatccaacg ttttgatggg cgggaattca ctgggaagtt
     1381 cgggatgaac gatttgaaaa atgcgctcag gacgggtcag atctttgagg cggtgctcac
     1441 tcagatctcg caaggttatt tccgcgggta gttgctcgcg gacaagatct gctgtagtat
     1501 ttgagagcag aacctgcccg ccatttgcaa cagacattaa tcgcgccgca cgattcaacg
     1561 tcccgccata ataatcattg ttacgcaatt cagcttcgcc tgtatgaatc cccatgcgga
     1621 ctttcaatga caatgatgaa ggatgaagaa agaaggagga atgaagttcg cgctgcgctg
     1681 taacggatgc gttgatgcca tccatggcag ttgcaaacac tgcatgaaac ccatcccccg
     1741 tggttttgat gacatgtcca cgattcgatt caatggcatc tttcaagatc gaatcgtgtt
     1801 ttgcaagcgc ggtcttcatc gcttcgggat gctgctccca aagtttggtt gagccttcga
     1861 tatcggtaaa aagaaaggtc actgtgccaa acggaagttc gcgcatgttc catcctcaca
     1921 agcagcccct ttgacaaaaa ccacatccac gtggatattt ggactattct aatccaatat
     1981 agttaagtac aataacctta atgcatattc ctgtacgtcg aatccaatag gcgccaaatg
     2041 gatcgaattc tcgaaacgtt caagatcaaa cccggcgaag agcgcatgtc tgcgctgttg
     2101 atggggctga tgctgatcac tgcagcaggc ggagcgatcg gtgggaacgc caccgaagcc
     2161 ttgttctttg cacgcttcgg tgtggaattc ctgcccacca tgtatgtcat tctgggcgtg
     2221 ttcaccttcc tcacctccat ggcaattacc atcgtcatgg ggcgtttttc aaaacagcgg
     2281 ctgttcacca cccttccatt tttcatcggg ctgatattga tcgtcgagtg gatcgtactt
     2341 cgtttcgata ttcgctggtt ctttgccgcc atgtggctgg gcatgaacgt catcaacagt
     2401 ctgcaagggc tggccatctg ggggttggcg ggtacagcct gtgatacacg gcaagccaaa
     2461 cggttgttcc ctctctttag tgcaggcgga atcttaggcg cggtgcttgg cggcctgttg
     2521 actcaatcat tggcgcgctg gctgcattca gaaaacctat tgctggtttg ggctggcact
     2581 ttattcatcg ccttccttct tggccgtgct ttgatcggca ggtcggttgg ccccaatatc
     2641 acctcagcac ggataaaaca acccggtatc ttcgtcgaaa tgcagcgcgg atacaacttc
     2701 gtgcgcagat ccccgatcat gcaatgggtt tcatacagtg ccattttatt ttcggtctgt
     2761 tatttctcac tcgctcttcc attttcacgc ggtgccaccg ctcaattcac caatgccgac
     2821 cgccttgcgg ggtttctggg gttatttcaa agcatcacca ccattgttgc tttaatcgtt
     2881 tcattgtttt ttgccaatcg tcttttcgcc cgctttggca tcatgccgat gcttttgctc
     2941 ttcccgatca tttacttcgt cgggtttggt gtgctttcat tctccgcacc ttttgcaatt
     3001 ttagtttctg tgcgttttat gcaaatgatg tggatgcagg ggattgcagg cacagcctgg
     3061 caggcacttt tcaatgtggt gccgtctgaa caacgcgatc aggtccgaac attcattggc
     3121 ggtgtgcctg aacaggcggg cacttttatt gcggggttaa ttcttgtggt cggcgagcaa
     3181 accctggccc cggggcagtt atattttatc gggctcatcg ccgccgcgtt tttgatctac
     3241 atgctctggc gtgcgagtcg cgattacagc attgcattgg tcgaggcgct tcgcgcaggt
     3301 caacctcaaa tgttcgtcag cgaagaggaa cccttcggtg gtttcagcac ggatgcaaca
     3361 gcagttgctg cggctcttaa aggaatgagc gatcccgatc gcctcacccg cctggtgtcc
     3421 attgagatcc ttgggcactt atccactccc aaagtgatcg ctggcatggt ctcggcactt
     3481 acagatgaag acgccatgat ccgctcttct gccctgcgcg gcctgactca cgcccgggca
     3541 tcctccgccc ttctggagat tgccgcgagt ctttccgacc ctgagcctga ggtgcgcctt
     3601 caagcagtag actcccttcg gcagttggct cagcatccac gcggggtatc cgcccacatc
     3661 gaacccctgc tgacagatca agacccgctc gtccgcaccc gctctgctca aactttgttg
     3721 agcttcggtg aacaccccaa agcaaaaagc atcctgcatg aaatggcggc aggcacagac
     3781 atcacctccc gtacacatgc attgaatgca ttggctgaat gcagggatga atctgccttt
     3841 gaattgggac tatcagccct cgacgatgcc gcccccatca tacgaaagac cgcgccgcga
     3901 ttgttggtga gcatcgaccc gcagcgcgca ctggaaccca tccttcatca cctcaacgat
     3961 gacgacagcc acgtccgcaa ggcgcttgca gaagcgatcg ggaacatcgg cctccccgca
     4021 ctggattcat tgctagcagc cttgaccaac ccaagttatg aagacggcgc cttgacagca
     4081 cttgaactcc tgcccgccca gaaagcaagc cagcaaatcc gcgtccatgc aatgaataca
     4141 gccagatccg cactgcgcta tcatcaactg gcattggggc tcgctcaaaa gttcgggcgg
     4201 accatttcag gtgacggccg ttatcaattg ctgatcgagt cattgcacga caaagcccac
     4261 cgcagcggac acaattccct gcgggcggtt ggtttgctga aagaccgtca gaccatcggc
     4321 attgccattg aaaacctgcg cagccgtgaa tccgctcaaa gggcgaatgc gctcgaagtc
     4381 cttgaatcaa ttggcgaggt ggaaattatc cgcccgctgc tggctttatg ggaatctgga
     4441 gagtcgtctt ccgcatctct gcccgatcaa tggctgatgg agctgcttca tgatccctac
     4501 tcatggctgc gtgcctgcgc tgttttggca gccgccgaat ctgatgatga gaagattgtt
     4561 ctcactcttg gatctctttc aagatcagac cccgatgcaa ccgttcgcga tattgccaat
     4621 aaagtaattt caggaggtca ttcaatggat acacttgcca cccttccgct catggaacgt
     4681 attcttttcc tgcggcgcgt acccatcttt gcagatcttt cccccgctga cttaaagcaa
     4741 gtagcggcga tcacaaccga ggccgttttt gccgatggag atatccttgc acatcaggga
     4801 gaattcggcg atgaaatgtt cattgtcgtt tccggggaag tgaatgtact gatcgcctcg
     4861 gatgatcaaa aggaatcccg ctacatcgca aaaaggtcaa ctggcgatta tgttggggaa
     4921 atgtccatta tcagccgtga accgcgtatt gccaccctgg tcgcatcggg gcaggtgcgg
     4981 gccttgtgcg tgggtcgaaa acaatttgaa ggcatcctgc gcgaacgccc cgagatcgga
     5041 atggcattaa tgcgcaccct gtgccaaagg ctaaaggaag caagcctgca acaaacaaac
     5101 taataccatt tctggcatcg attaaataaa aaggaggctg tcactcttat ggcgacagcc
     5161 tccttgcata cttttacttg accaaattgc gcagcaccgt gtgcaggata ccgccgttgc
     5221 ggtagtagtt cacttccaca tcggtgttca acagcgcggt ggctttgaac tcgatcactt
     5281 tgccatcggc tttcttcgcc ttgaccatca actctgcctt gggcttgatg ttgttatcga
     5341 ggccttcgat gtcaaagact tcgcttccat ccagaccgag ggactcggcg ttttgacctt
     5401 cagcgaatct cagcggaagc acgcccatgc cgaccaaatt cgagcggtga atgcgttcga
     5461 aagattcagc gatgacggcc ttcacaccct gcagcatcgg tcccttggcg gcccagtcac
     5521 ggctggagcc agtgccgtat tccttgcccg cgaggacaat gctcggcacg ccttcggctt
     5581 gatatt
//
LOCUS       DAOQJC010000194         5475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_8603_length_5475_cov_10.447417, whole genome shotgun sequence.
ACCESSION   DAOQJC010000194 DAOQJC010000000
VERSION     DAOQJC010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8603_length_5475_cov_10.447417"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /gene="hutU"
                     /locus_tag="PLE39_18290"
     CDS             complement(<1..1225)
                     /gene="hutU"
                     /locus_tag="PLE39_18290"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNE70340.1"
                     /translation="MSTPRVIRSPHGTQLTCKNWLSEAVYRMIQNNLDPEVAEKPEDL
                     VVYGGRGKAARNWEAFDAILESLKNLEADETLLVQSGKPVVVFKSHKDAPKVLIANSN
                     IVPHWATWEKFDELDRKGLIMYGQMTAGSWIYIGTQGILQGTYETFGALAKLKGWGSL
                     KGKFVLTAGLGGMGGAQPLSITMNEGVGLIVEVDPERAERRRAIGYVDMVVDNLEEAM
                     TLVEENVKNGTPKSIGLIGNAADVYSELAARGIIPDVVTDQTSAHEALFYVPSGISLT
                     AANQLRVSDPEKYKKMAMDSMAKHVQAMLAFQRAGAEVFDYGNNLRQQAYNHGVTDAF
                     EFPGFVPAYIRPLFCEGKGPFRWVALSGDPEDIYTTDKAIMELFPDDAHLHRWMKMAR
                     EKVPFQGLPSRICWLG"
     assembly_gap    1249..1348
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1349..2184)
                     /locus_tag="PLE39_18295"
     CDS             complement(<1349..2184)
                     /locus_tag="PLE39_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70341.1"
                     /translation="MTHRKTSFFALILLVTVTACVIPGLSTPTAPPLFAPTTDPGRLE
                     TMVAETVVAAIAETEQSQPTPTRIPTSQPTSTATPEPITAGSTLTLQEDGTTTFLDER
                     AGYQITIPDGWLAVRVDEQEYYDAWNLAEATDPNIQAILLGMKEQDPNVQRLRAIDTQ
                     DGHIQSDFVTDISFIWNEQGKFETIEDLQAIASELPNAPSAFRFEVATVQTIIAPSGI
                     QFGVIEATSSFTNTSGDLVNVYLKQIVFKAKTGTQSIILLTVEGLKETTLPAFDGMIE
                     TI"
     gene            complement(2261..2674)
                     /locus_tag="PLE39_18300"
     CDS             complement(2261..2674)
                     /locus_tag="PLE39_18300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNE70342.1"
                     /translation="MQITRQADYAVRAVLHLARTGDQRTATSTIAEEQHIPPSFLAKI
                     ISQLSIAGLLHTSRGARGGVTLAREAKEITLLEVIEAIDGPIQLNECVGENGICSFDE
                     SCPLRPVWCDAQEELVGRLKGTNFADMIAKGQTSV"
     gene            complement(2872..3720)
                     /locus_tag="PLE39_18305"
     CDS             complement(2872..3720)
                     /locus_tag="PLE39_18305"
                     /inference="COORDINATES: protein motif:HMM:NF028425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HNE70343.1"
                     /translation="MLRKLILLIAILALASMACGFNINIPDAPKPGPQVTDDINVKAP
                     KADDINLKLSFGAGEMILAPGADQLVAGTASYNYSAFKPNINTDGGNVSITMSEVDFK
                     TFPNFNEFKNKWDFELGDNPMNLSIESGAYDGTFELGGLALENLSIKDGAANVVLSFS
                     EPNLSEMSTFVYETGASDVKMTGLANANFSLMDFSSGAGDYTLDFSGELQRDASIKIS
                     SGLSNIIIVVPEGVNAVVTMDGGASNISVGSSWSMNGGTYMQKGEGPTLTFVIEIGAG
                     NVTLTH"
     gene            complement(3790..5208)
                     /gene="radA"
                     /locus_tag="PLE39_18310"
     CDS             complement(3790..5208)
                     /gene="radA"
                     /locus_tag="PLE39_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNE70344.1"
                     /translation="MAKTHTRYVCQQCGRVAASYMGKCPQCGSFSSMVEEVIHDEPVS
                     KGSSNARGLSGRSEPRPISEVGGEAEDRVALPITEFSRVLGGGIVLGSIVLIGGDPGI
                     GKSTLMLQMAMEMAGIQKVLYVSGEESERQVKMRAVRLLGGKKELPKNLLLVTETNLE
                     VILNHVNELKPDLLIVDSIQTVYLSELDSSAGSVSQVRECASQLRELAKSSGVSVFMI
                     GHVTKEGAIAGPRVLEHIVDTVLYLEGDRFQAYRLLRSVKNRFGATAEVGVFEMREGG
                     LVEVTNPSEAFLAERLVNAAGSAIAVTMEGTRPILVEIQGLTSPTQFGNARRTPNGVD
                     FNRLLLISAVLTRRVGLKLSEQDVFVNVVGGIQIDEPAADLAVAAAIASSWKDVPVRA
                     EAVLIGEIGLAGELRMPGQMVARLREAQKLGFKAAIVPKAIRQGEAYPKGIEIVEVRS
                     LDQALNTALMKDGLASKARKSE"
     gene            5366..5439
                     /locus_tag="PLE39_18315"
     tRNA            5366..5439
                     /locus_tag="PLE39_18315"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5400..5402,aa:Arg,seq:gcg)
BASE COUNT         1288 a         1386 c         1371 g         1330 t
ORIGIN      
        1 aaccaagcca gcaaatgcgc gagggcaatc cctgaaacgg aactttctct cgcgccattt
       61 tcatccagcg gtgcagatga gcatcatcgg ggaagagttc catgatggct ttatcggtgg
      121 tgtaaatatc ttcggggtcg ccggagagcg ccacccagcg gaaggggcct ttgccttcgc
      181 aaaacagcgg acggatgtag gcgggcacaa agccggggaa ttcgaaggca tctgtcacgc
      241 cgtggttgta ggcttgctga cgcaggttat tgccataatc gaacacttcc gcgcctgcgc
      301 gttggaaggc cagcatggcc tgaacgtgtt ttgccatgga gtccattgcc atctttttat
      361 atttttcggg gtcactcacg cgcagttgat tcgcggcggt gaggctgatt ccagatggga
      421 cataaaataa tgcttcgtgc gcagaggtct ggtcggtgac cacatcgggg atgatcccgc
      481 gcgcggcaag ttcggaatat acatcggcgg cgttgccgat caggccaatg gatttgggcg
      541 ttccgttctt tacattttct tccacgagcg tcatggcttc ttcgaggtta tccacaacca
      601 tatccacata gccaatggcg cggcggcgtt cggcgcgttc ggggtccact tcaacgatca
      661 ggccgacacc ctcgttcatg gtgatcgaaa gcggttgggc gccgcccatg ccgccaagtc
      721 ccgccgtcaa aacaaattta cctttgagag atccccaccc cttaagtttg gcgagtgcgc
      781 caaaggtttc gtacgtgccc tgcaaaattc cctgtgtgcc gatgtagatc catgaaccgg
      841 cggtcatctg cccgtacatg atcagtccct ttctatcgag ctcgtcaaat ttttcccagg
      901 tggcccaatg cggcacgatg ttggagttgg cgatcagaac tttgggcgca tccttgtgcg
      961 atttgaaaac gaccaccggc ttgcccgatt gcacaagcag ggtttcatct gcttcgagat
     1021 ttttcaaaga ttcaaggatt gcatcgaagg cttcccagtt gcgggcggct tttccgcgcc
     1081 cgccgtaaac gacgaggtct tcgggctttt cggccacttc gggatcgagg ttattctgaa
     1141 tcatgcgata gacagcttcg ctgagccagt tcttgcaggt tagctgtgtt ccatgcggtg
     1201 agcggatgac gcggggtgtg gacattgggg aatctccttg gtgaaattnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt aatcgtttcg atcatcccgt caaatgcggg
     1381 cagggtggtt tctttcaaac cttccacagt aagcaggata atggattgtg ttcccgtttt
     1441 ggctttgaaa acgatctgct tcaagtacac atttaccaga tcaccggaag tatttgtgaa
     1501 cgaagaagtg gcttcgatca caccgaattg tatcccgctc ggagcgatga tggtttggac
     1561 agtggcgact tcgaacctga aagcagaggg ggcattgggc agttcactgg caatggcttg
     1621 caggtcttcg atggtttcga atttcccctg ttcgttccag ataaaactga tatcggtcac
     1681 aaaatcgctt tggatgtggc cgtcctgcgt gtcaatggca cgcagacgct gaacatttgg
     1741 gtcctgttct ttcatgccca gaaggatcgc ctggatgttt gggtctgtgg cttcggctaa
     1801 attccaggca tcgtagtact cctgctcgtc cacgcgtacg gcgagccagc catcagggat
     1861 ggtgatctgg tacccggcac gttcatcgag gaaggtggtt gtgccgtctt cctgtaaggt
     1921 gagcgtactc cctgctgtga tgggctccgg ggtggcagtg gaagtaggtt gggaggtcgg
     1981 gatgcgtgtg ggggttggtt gtgattgttc agtttcagcg atggcggcga caactgtttc
     2041 agcgaccatg gtttccaggc ggccgggatc cgttgtcggc gcgaagagag gcggggccgt
     2101 cggggtggaa agtcccggta tgacacaggc tgtgactgtt accagaagga tcagtgcgaa
     2161 gaaagatgtc tttctatggg tcatggattc gttccttttc gcaaaaataa aaaagcctcc
     2221 cggctattat agccgagagg cttcaggatg ctttcgcgct ttagacggaa gtctgtcctt
     2281 ttgcgatcat atccgcaaaa ttggtgcctt tcaacctgcc caccagttcc tcttgtgcat
     2341 cacaccatac tgggcgcaag gggcatgatt cgtcgaagga gcaaatacca ttttcgccaa
     2401 cacattcatt aagttgaatg gggccgtcaa tggcttcaat gacctccaaa agggtgatct
     2461 cttttgcttc acgggcgagt gttacgccgc cgcgcgcacc gcgagatgtg tgcaacaacc
     2521 ctgcaatgga taattgggag atgatcttgg ccaaaaagga tggcggaata tgctgttctt
     2581 cagcaatggt gctggtggct gtgcgctgat cgccagtgcg ggcaagatgg agcacggctc
     2641 gaacggcata atcagcctga cgtgtaattt gcatgataac ctctcttaaa acaggtaaac
     2701 tttaccgata atcacgattt tattgtgtaa ggtagtacaa ggcaagtgat gtaggtcata
     2761 gataaattat gaccttttgc tccgaaatgg ggtggaaacc ccatacaggt ttaaacgaaa
     2821 taaaaatgcc cgcgtcaaag gtttgatgcg ggcatttttg cgcaatagct tttagtgtgt
     2881 caaagtgaca tttcctgcgc cgatctcaat tacaaaggta agagtggggc cttcaccttt
     2941 ttgcatgtaa gtaccaccat tcatgctcca actcgaaccc acgctgatat tcgaggcacc
     3001 gccatccatt gtaaccacgg cattcacacc ttcaggaaca acaatgatga tgttgctcaa
     3061 tccgctggaa attttgatgg aggcatcacg ctgcaattca ccagagaaat cgagagtgta
     3121 gtcacccgca ccggatgaga aatccatcag gctgaaattg gcattggcaa ggcctgtcat
     3181 ttttacatcg gaagcacctg tttcatacac aaaggtggac atctcggaca ggttgggctc
     3241 tgaaaacgaa agcactacat tggcagcgcc atctttaatg gaaagatttt cgagggcaag
     3301 cccgcccaac tcaaatgtac cgtcataggc gcccgattcg atgctcaagt tcattgggtt
     3361 gtcgcccagt tcaaaatccc atttgttctt gaattcgttg aaattcggga aagttttaaa
     3421 atccacttcg ctcattgtga ttgaaacatt cccgccatcg gtattgatat tgggtttgaa
     3481 tgcagagtaa ttgtagctgg cagtacctgc caccagttga tctgcgcccg gcgcaagaat
     3541 catttcaccg gcgccaaaag aaagctttaa attaatatca tctgccttgg gggccttgac
     3601 gttaatatca tcggtcactt gcggaccggg cttgggagca tccgggatgt taatattaaa
     3661 tccgcaggcc atgctggcca atgcgaggat cgcgatcaat aagattaatt ttcgtaacat
     3721 ggaaaatacc tcctcaggta atacagtgtc tgaggaggta tacggctgat cttttatttg
     3781 gttgcagggt tattcggatt ttctggcctt tgatgcaaga ccgtctttca tcaacgccgt
     3841 attcaaggct tggtccagcg agcgcacttc aacaatttcg atccccttcg gataagcctc
     3901 accttgacga atggctttgg gaacaatggc ggctttgaat cccaatttct gagcctcgcg
     3961 cagtcgtgcc accatttgac cgggcatgcg taattcacca gccaggccaa tctcaccaat
     4021 caatacggct tcggctcgca caggtacatc tttccatgaa gaagcaatgg ctgcggcaac
     4081 agcaagatcg gcggcaggct catcaatctg tatgccgccc accacattca caaaaacatc
     4141 ctgctctgaa agtttcaaac ctacacggcg ggtcaataca gccgatatca acagcagacg
     4201 attaaagtcc acgccgttgg gggtgcggcg tgcatttcca aattgggttg gggaggtaag
     4261 tccctgaatt tcaaccagaa tgggacgtgt gccttccatg gtcacagcaa tggccgagcc
     4321 agctgcattg accagtcgct ctgcaagaaa tgcctcagat ggatttgtca cttcaaccaa
     4381 tccgccttcg cgcatttcga aaacccccac ttccgcagtc gcgccaaagc ggttctttac
     4441 cgagcgcagc aaacgataag cctgaaagcg atccccttca agatacaaca cggtatccac
     4501 aatatgttcc aacacacgcg gaccggcgat cgcgccttcc ttcgtcacat gcccgatcat
     4561 aaacacagat acacccgaag acttcgccag ttcacgcaac tgcgaagcac actcacgcac
     4621 ctgcgaaacc gaccctgccg acgaatccaa ttcagacaag tacacagtct ggatcgagtc
     4681 aacgatcaac aggtcgggct ttaattcgtt gacatgattc aagatcactt caagattggt
     4741 ctcagtcacc aataataagt tttttggcaa ttcctttttt ccacccagca agcgcacagc
     4801 gcgcattttt acctgccgct ccgattcctc accggaaaca tacagcacct tctgaatccc
     4861 ggccatctcc attgccattt gcagcatcaa agtggatttg ccaatgcccg ggtcaccccc
     4921 aatgaggaca atggaaccga gcacaattcc gccgccaagg acacgtgaaa actccgtgat
     4981 cggaagcgcc actcgatctt ccgcttctcc tccgacctct gaaatgggtc gcggctccga
     5041 acggccactc agcccacggg cattggatga tcccttggac accggctcat cgtggatcac
     5101 ttcctccacc atgctgctaa aggacccgca ctgtggacat ttgcccatgt aagaagcggc
     5161 tacacgtccg cattgttgac aaacatatcg ggtatgtgtt tttgccatat tcgatctccc
     5221 ataaggtgag gtagggcgag tataacagaa tttatgttct gttttcaaag cccgcccatt
     5281 tcacaaccac cgcacaccga cttaaaaacc aaaaacgctt gaccatcgtg ccacattaca
     5341 gtaaaatacc acccgcataa aaaacgcttc cgtagctcag tggattagag cgcttgcttg
     5401 cggagcaata ggtcgcaggt tcgagtcctg ccgggagcac aaaaaaccgt ctaaatacag
     5461 gcggtttttt tattt
//
LOCUS       DAOQJC010000195         5447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_8676_length_5447_cov_8.338835, whole genome shotgun sequence.
ACCESSION   DAOQJC010000195 DAOQJC010000000
VERSION     DAOQJC010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8676_length_5447_cov_8.338835"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..2011
                     /locus_tag="PLE39_18320"
     CDS             248..2011
                     /locus_tag="PLE39_18320"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HNE70345.1"
                     /translation="MEAIVFRVRQTLIFLILTASLLIGQSPAFAAPASSIAETTILAA
                     SLPVSGTPVPELDVFDQTMQNYMNARNIKAGVLVVMKDGAIVLEHGYGWKDKTLQTPI
                     QPDAMFRLASIIKPVTEASILKLTWEGKISLDDKVFCVTGSPSNCELTISPFGTPDSR
                     LKDITIQNLMDHEGGWDRDLSGDPMFKSITIANALGVASPPSKEDTVRYMMGQPLDHT
                     PGTQYAYSNFGYLLLGLIIEKNTGQTYTAYIQDNIFNPLGVADTEIELGRTLPQYRNP
                     REPWYSEPLYSGAPNVFNPSETVPWPDGGWYLEAMEAHGGMISTGRAMAEFLNAYWIN
                     GEPRNGNGQTWWFYGSLDGTYTLAYQRPDGVNLVALFNQRADASYSDYSVIKSLLDTA
                     TDSVTQWPTSTIFTDVPSSYWAAPYIESLYNAGITGGCSTSPLMYCPESTVTRAQMAV
                     FILRGIHGSAYVPPAATGTVFTDVPADYWAASWIEQLAVEGITSGCGGTSYCPDATVT
                     RAQMAVFLLRGKYGSTYTPPAATGTVFADIPSDYWAANWIEQLATEGITSGCGGGLYC
                     PDAEVTRAQMAVFLVKTFNLP"
     gene            complement(2057..3109)
                     /locus_tag="PLE39_18325"
     CDS             complement(2057..3109)
                     /locus_tag="PLE39_18325"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE70346.1"
                     /translation="MKSSTLPFILQALIAALFFGASAPIAKLLLGDNIAPIFLAAFLY
                     LGSGAGTFLFRLTQRMRSKDAEADIKSPDIKWLAGAIIAGGILAPIILMISLQNTPAS
                     TASLLLNFEGVGTTLIALLFFKEAIGRRAWIAILVITLASIFLSTNFDEGFGLSLGAL
                     GIILACVLWGVDNNFTRNISGKDPLAIVAWKGLVAGTFSFFLAYFLGNTLPSLTVILG
                     TLVLGFVSYGLSTMLFIRSMRGLGAARTSALYGTAPLAGVLLSIFLFGEIPSFSFVIA
                     AVLMIGGALLLINEEHSHSHTHTAMFHEHSHSHDDSAHGHDTAKGVHSHEHEHPAEEH
                     EHDHMPDIHHRHGHEE"
     gene            complement(3106..3444)
                     /locus_tag="PLE39_18330"
     CDS             complement(3106..3444)
                     /locus_tag="PLE39_18330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70347.1"
                     /translation="MQNFTDVKLEIFAPQEYALQLRHELAKIGVGKIGNYDHCFAIYP
                     VQGYYRPLEGANPFGGEVGKISSGVEYKIEVNCRRELVNEAIKMVKRIHPYEEPLINV
                     IPLANHLFIK"
     gene            complement(3459..4286)
                     /locus_tag="PLE39_18335"
     CDS             complement(3459..4286)
                     /locus_tag="PLE39_18335"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE70348.1"
                     /translation="MSVAEIRAQDASAAKFAGPPPKDVQIERVNIDGIDAAWIRPSGA
                     ERTKTLLHIHGGGYVTGSIPSYLRMCILMAQMLNINVLLPAYRLAPEHPFPAAMEDVL
                     KIYRRLLNDGFQPKDIFISGDSAGGGLCVAAVIALRDQKEALPAAVLCMSPWVDLTMQ
                     GQSHVTNPKSEAMLNAGTLREWALLYTNEKNFGSPLVSPIFADFQKFPPLLIQVSSDE
                     VLLDDARMLAEKARAGGADVTLKIWDGLWHVWQIVGDAIPESRMAIEEIGEFIRAYR"
     gene            complement(4409..>5447)
                     /locus_tag="PLE39_18340"
     CDS             complement(4409..>5447)
                     /locus_tag="PLE39_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70349.1"
                     /translation="KEIPMRVTRESLLRIAKETAQERAYNDKDIVAAYLTGSLLNVEP
                     MLGGTADIDIVFVHRSQPKQLREFVKLTPDFHLDISRRMKEEFKSPRELRGDPWLGYE
                     MYDPILLYEREKFFDFVQASLRAGFDFEQPPLMLQRCRKMLSHGRGIWMDLTEVDESS
                     VGPTEVRKYMKALFHALNAIVELSGPPIHERRLLLEFPARAEAAQQTSMVAGAFALLG
                     ANAVDAEKVKSWVADWKVAYSLASEKDGVDLRINPIRLNYYEKAIKAMLEGSIPLAAL
                     WPLFHTWTMAAEVLDGDHLKFWQTAAGELGLLGAGFVEKVDGLDHFIDDIEVVFDGIA
                     RENGLDVSTGL"
BASE COUNT         1391 a         1418 c         1309 g         1329 t
ORIGIN      
        1 cgcctcggca aaaatccccg cgactcattc cacgggtgag gctggttctg tttcggcggc
       61 atttccacct tacaagactc taacaactaa taaataagta ttgacgtcat tttttttcga
      121 ctaaaatcat ctgaaatccc gtcttgatag gaggttgcaa caaaataagt cgtcctgtac
      181 ccaataaaaa actctccaaa gttcaaaatg ccatatccaa taatatcacc ccaaataata
      241 aaaccatatg gaggctattg tgtttcgcgt ccgtcaaact ctcatttttc ttatactaac
      301 tgcaagcctg ctcattgggc aatcaccagc ttttgcagcc cctgcttcct caatcgctga
      361 aacgacaatc ctggctgcat cattgcctgt aagtggaacc cctgtcccgg agctcgatgt
      421 attcgatcaa accatgcaaa attatatgaa tgctcgcaac atcaaggctg gggttttggt
      481 cgtcatgaaa gatggcgcaa ttgtcctgga gcatgggtac ggttggaaag ataaaactct
      541 ccaaactccc atacaacctg acgccatgtt tcgtctggca agcattatta agccagttac
      601 cgaagcttcc atcttaaaac tcacatggga aggaaagatc agccttgacg acaaggtatt
      661 ttgcgtcact ggcagcccat ccaattgtga attaacaatc agcccatttg gcacaccaga
      721 ctcaaggtta aaggacatta caattcagaa tctgatggat catgaaggcg ggtgggatcg
      781 agacctttct ggtgacccaa tgttcaaatc aatcactatt gccaatgcac tgggtgtggc
      841 aagccctccc agcaaagaag acacggtacg gtatatgatg gggcagccat tagatcatac
      901 tcctgggact cagtacgctt attctaattt cgggtatctt ttgttagggc tgattattga
      961 aaaaaacacc ggtcaaacat acaccgcata tattcaagat aacattttca atccactggg
     1021 tgttgctgac actgaaatag aacttggacg cacactgcca caatatcgga atccgaggga
     1081 gccttggtat tctgaaccct tatacagtgg cgcgccaaac gtatttaatc catcagaaac
     1141 tgtaccctgg cctgatgggg gatggtatct ggaagccatg gaagcccatg ggggtatgat
     1201 ctccactgga cgtgcaatgg ctgaattcct gaatgcttat tggataaacg gtgaaccacg
     1261 taacgggaac gggcagacct ggtggtttta tggaagtctg gatggcacat atacccttgc
     1321 ctaccaacgg cctgatggag tcaatctggt tgcgttattc aatcaacgcg cagatgcatc
     1381 ttatagcgat tactcggtaa ttaagtcatt acttgataca gccacagata gtgtaaccca
     1441 atggccaaca tcaacgatat tcactgatgt accctcatct tattgggctg ctccatatat
     1501 tgaaagtctg tataacgctg gcatcactgg tgggtgtagc acctctccat tgatgtactg
     1561 ccctgagtct actgtcaccc gcgcgcaaat ggcggtattt atcctacgcg gcatacatgg
     1621 cagtgcatac gtgccacccg cagcaaccgg cacagtcttc actgatgttc ccgctgacta
     1681 ttgggcagcc tcctggatcg aacaacttgc tgttgaagga atcaccagcg gctgtggagg
     1741 tacatcttat tgtcctgatg ccaccgttac tcgcgcgcag atggctgttt ttctattgcg
     1801 tggaaagtat ggcagcacat atacacctcc cgctgctacc gggacggttt ttgctgacat
     1861 tccaagcgat tattgggcgg caaattggat cgaacaactc gcaacagaag gcattaccag
     1921 tggatgtgga ggcggtctat attgccctga tgctgaggtg acccgcgcgc aaatggcggt
     1981 cttcctcgtg aaaacattca acctgcctta atcataaaaa caggacatgt cgaaaattga
     2041 catgtcctgt ttttatctac tcctcatgcc catgccgatg atgaatatca ggcatgtgat
     2101 cgtgctcgtg ctcttcagcg gggtgttcat gctcatgcga gtggactccc tttgcggtgt
     2161 catgcccatg cgcggaatcg tcatggctat ggctgtgttc atggaacatg gctgtatgcg
     2221 tgtgtgaatg ggaatgttct tcattgatca atagcaacgc gccgccgatc atcaaaaccg
     2281 ccgcaatgac aaatgaaaac gatgggatct ccccaaagag aaaaatggac agcaacaccc
     2341 cagccagtgg agccgtgcca tacaacgcgc ttgtccttgc cgcacccaac ccacgcatcg
     2401 agcggatgaa caacatcgta ctcaagccgt agctgacaaa ccccaaaacc agcgtgccga
     2461 gaatgaccgt caacgaaggc agggtattcc caaggaaata tgccagaaag aaagagaatg
     2521 tccctgcaac caatcccttc cacgccacga tcgcaagcgg atctttgcca gagatattgc
     2581 gcgtgaagtt attatccaca ccccacaaca cacaggcgag gataattccc agcgcaccca
     2641 gtgaaagccc gaagccttcg tcgaaatttg tagagagaaa aatgctcgcc agcgtaatga
     2701 caaggatcgc aatccatgca cgacggccga tggcctcctt gaagaacaac aaggcgatga
     2761 gcgtcgtccc caccccttca aaattcaaca gcagcgaagc cgttgaagca ggtgtatttt
     2821 gcaggctgat catcaaaata atgggagcca ggattccgcc cgcaatgatc gcgcccgcca
     2881 gccatttgat atctggagat ttgatatccg cctcggcatc cttggagcgc attcgctgag
     2941 tcagcctgaa gagaaaagtc cctgcgccgc tgccgaggta cagaaacgcc gccagaaaga
     3001 tcggcgcgat attatcgccg agcaataatt ttgcgatcgg cgcactcgcc ccaaaaaaca
     3061 acgcggcaat caacgcttgc aggataaaag gcagggtgga tgatttcatt ttataaaaag
     3121 atgattcgcc agtggaatta cattgatcaa cggttcttca tacgggtgaa tgcgtttcac
     3181 catcttgatc gcttcgttga ccaactctcg cctgcaattg acctcgatct tgtactcgac
     3241 gccggagctg atctttccga cttcaccccc aaaaggattc gcgccttcga gcgggcggta
     3301 atatccctgc acagggtaaa tggcaaaaca atgatcgtaa ttcccaattt tgccaacacc
     3361 gatcttcgcc agttcatggc gaagctgcaa ggcgtattcc tgcggggcaa atatttcaag
     3421 ttttacatcg gtaaagtttt gcatacttcc ttccataatc agcggtaagc acggataaac
     3481 tcgccgatct cttcaattgc catgcggctc tcagggattg cgtctccaac aatttgccag
     3541 acatgccaca agccatccca gattttaagc gtgacatccg caccgccagc tctggctttt
     3601 tctgcaagca tccgcgcatc gtcgagcaaa acctcatcac tgctgacctg gatcaacagc
     3661 ggcgggaatt tttgaaagtc tgcaaaaatg ggcgagacca gcggactgcc aaagtttttt
     3721 tcgtttgtat acaacaatgc ccattcgcgc aaagtgcctg cgtttaacat tgcttcggat
     3781 ttcgggtttg tcacatgcga ttgcccctgc atggtgaggt cgacccatgg agacatgcac
     3841 agcactgcgg caggaagcgc ttctttttga tcccgaagcg caatcaccgc tgcaacgcat
     3901 aatccgccgc ccgctgaatc gcctgagatg aagatgtctt tgggttgaaa cccatcgttc
     3961 aataggcggc ggtatatttt cagcacatcc tccatcgccg ctgggaaggg atgctcaggc
     4021 gcaaggcgat atgcgggcaa aagcacattt atgttcaaca tttgcgccat caggatgcac
     4081 attctcaagt aggatggaat ggaacctgtc acatacccgc cgccgtgaat gtgcaaaagc
     4141 gtttttgttc tttccgcacc tgacgggcgt atccaggcgg cgtcgatccc atcgatgttg
     4201 accctctcga tctgcacatc tttgggcggc ggccccgcaa atttggcggc actggcatcc
     4261 tgtgcgcgaa tctcggcgac gctcatgtgt tttttatata ttttgcggat gacaaatttc
     4321 cagatatgac ttctcaaact acgtttcatt gttttctcac atggttataa aaaacttccg
     4381 aagccttaag acttcggaag tttgaaattt acaatcccgt tgatacgtcc agcccgtttt
     4441 cgcgggcaat gccatcgaac acgacctcga tgtcgtcaat gaaatggtca aggccatcca
     4501 ctttttctac aaagccagca ccgagcaggc ccaactcacc tgccgcagtt tgccagaatt
     4561 tgagatgatc gccatccagc acttccgcag ccattgtcca ggtgtggaag agcggccaga
     4621 gcgccgcgag gggaatcgaa ccttcgagca tggccttgat ggctttctcg taatagttca
     4681 agcggatggg attgatgcgc aagtccacgc catctttttc actggcgagg gaataggcaa
     4741 ctttccaatc ggcgacccag ctttttactt tttcagcatc gaccgcattg gcgccgagca
     4801 gggcaaatgc gccagccacc atgcttgtct gttgggcggc ttcggcgcgg gcggggaatt
     4861 ccagcaaaag cctgcgctcg tggatgggag gtccgcttaa ttcgacgatt gcgttcaagg
     4921 cgtggaaaag cgccttcata tatttgcgaa cttccgtcgg gccgaccgag gattcgtcca
     4981 cttcggtcaa gtccatccag atgccgcgtc cgtgagagag cattttgcgg cagcgttgca
     5041 gcatgagcgg tggttgttcg aagtcaaaac ctgcgcgcag actggcttgc acaaaatcga
     5101 agaacttttc gcgctcatac aacaaaatgg ggtcgtacat ttcgtagccg agccacgggt
     5161 cgccgcgcaa ttcacgcgga gatttgaact cttctttcat tcgacggctg atatcgagat
     5221 gaaaatcagg tgtcagtttt acgaactcgc gcaattgttt cggctgggat ctgtgaacga
     5281 agacaatgtc aatatcagcc gtgccgccaa gcatgggttc aacattgagc agggagcctg
     5341 tcaggtaggc ggcgacaatg tccttgtcgt tgtaggcccg ttcctgggcg gtctcttttg
     5401 cgattcgaag aagtgattca cgtgttacgc gcatagggat ttccttt
//
LOCUS       DAOQJC010000196         5369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_8864_length_5369_cov_9.229770, whole genome shotgun sequence.
ACCESSION   DAOQJC010000196 DAOQJC010000000
VERSION     DAOQJC010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8864_length_5369_cov_9.229770"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLE39_18345"
     CDS             complement(<1..625)
                     /locus_tag="PLE39_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNE70350.1"
                     /translation="MANKKVRVAIIGVGNCASSLVQGVQFYKNAKEDETIPGIMHTQL
                     GEYHVRDIEFTLAIDINATKVGKDLSEAIFAEPNNTYKFCDVPHLGVPVVRGMTHDGL
                     GKYLSEVIKKAPGSTAEISKLLKETKTDVVINFLPVGSEMATKWYVEQILEAGCAFVN
                     GIPVFIASSEYWGKRFADAGLPILGDDIKSQVGATIVHRVLTSLFVDR"
     gene            737..2002
                     /locus_tag="PLE39_18350"
     CDS             737..2002
                     /locus_tag="PLE39_18350"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNE70351.1"
                     /translation="MAMDQVTLLARLLEVTRNLSTMVDVDAYLQSILSAATELTGSET
                     ASLMEYDETTQEFFFRFVPWFHRDLIKDARVPLNGSAAGWSLLHSKPLIINDVKNDER
                     HFNKVDEMANFVTRSMLVVPLLLHGRPMGVFEVINKANDAPYTDEDILALETLASLAT
                     TAIQNNLLENSIQSSQNESRDLDRLKNEFIAITSHELRTPLGLILGHATFLRELVGND
                     FQEQVEAIIRNASRLKEIIESLSTVDNYQTGGASLRSRRVSMNRVIEDVIASFRDIAK
                     KKGVSLKSDLDRNQELLVDADGSKIAIVLSNLVKNAVTFTNDGGHITVRGSLDSSFVK
                     VVVEDDGVGIPARDLPHVFERFYQVESHLTRRHGGMGLGLSVAKVMIEMHGGRIWAES
                     LEGLGSKFTFLLPLHPKVNEPDPVSPFVE"
     gene            2099..3286
                     /locus_tag="PLE39_18355"
     CDS             2099..3286
                     /locus_tag="PLE39_18355"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNE70352.1"
                     /translation="MQSINEQFELIRQSATVAMADRILALKASGRDIVGLQVGDPDFG
                     THPAIAEAAMKAIQSGLTHYGPSRGYPELRTAVANKLMRDEGVTYDPASEILITHGGI
                     HAYYLAMQSILNPGDDVLIPDPSWATHSNMAIMLRGNVIRIPAPAENGFIPHFESWLK
                     ALTASAKPRVIVLNYPSNPTGAYPTRDYLQKLQDFAKEHSLWIVSDEVYGSLFYEGRP
                     TSAAAIEGAKERTLIINSLSKSYAMTGWRVGFLAAPAHIIENALKAGQNSITCVAPFI
                     QKAAAFALTDPAMQEFTEEMRTGYSRRRDLVLRLSHQLESEKVKVIPPQGAFYFFLDL
                     RALNMSSVEMCEKILDEVGVGLVPGSAFGEQGEGFIRMTIAASDADVEAGFRKIVEWA
                     ERQ"
     gene            3299..4111
                     /gene="pheA"
                     /locus_tag="PLE39_18360"
     CDS             3299..4111
                     /gene="pheA"
                     /locus_tag="PLE39_18360"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNE70353.1"
                     /translation="MKVSFQGEPGAYSEQAVFNYFGEVETVPCESFDVMFDSVVSGKS
                     DVALAPIENSLAGSIHQNYDLLLRHDLHIVGEYFLRVRHCLIAMPGAKMEDIKKAISH
                     PQALGQCAAYLRGRGIKPETVYDTAGSVKMLKASGAMDTAAIASRRAAELYGMQILEE
                     GIEDNSENYTRFLAVQREAAFPKGEAKTSIVFTLKHVPGSLFKAMSVFALRDIDLTKI
                     ESRPLQGKPWEYLFYIDFIGSADDDTPRRALEHLGEYATTLRVLGSYPRFRG"
     gene            complement(4101..4520)
                     /locus_tag="PLE39_18365"
     CDS             complement(4101..4520)
                     /locus_tag="PLE39_18365"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNE70354.1"
                     /translation="MSWEFTHPPVKVWQAWTDQTITKLWFGSDPNGKVLDAKLDVQVN
                     GSFSVTFANSDGAEFTAQGIYKEVNEPNRLVFTWGWANQPEVHELISLEFSPDGAGTL
                     MTFEQSKVDPSTSMHNYAEGWRSTFQKLERALGTINP"
     gene            complement(4555..>5369)
                     /locus_tag="PLE39_18370"
     CDS             complement(4555..>5369)
                     /locus_tag="PLE39_18370"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE70355.1"
                     /translation="KQEKYKEAIDTYEEALPIFEKQHEPKSIASIWHQIGIVHQEAGN
                     YESAEAAYRRSLEIKSQNNDRAGQANSLINLGVLYNDKLNRPEEAVVFYRQSADIYVE
                     IGDLRYEGFTRNNIANTLQQLKRYDEARAEIMRAIECKSQIGLAAEPWKSFDILHNIE
                     ESTGNSTAARAAWEQARDAYLAYRRQGGYASQGAGGQFIDQILGLIQQGKSDEIQNLL
                     NQYSDAPDYFKKFMQIVVDILNGSRDKALGDDPALSYSYTAEVLFLIERLGG"
BASE COUNT         1300 a         1349 c         1338 g         1382 t
ORIGIN      
        1 ctcgatctac gaagaggctg gtcagcacac gatgaacgat cgtcgccccg acctgactct
       61 tgatgtcgtc accgaggatg ggcaggcccg catcggcaaa acgcttgccc caatattcag
      121 aagaagcgat aaagacgggg atgccattaa caaatgcgca gcccgcttca agaatctgct
      181 ccacatacca tttggttgcc atctcggaac caacgggcag gaagttgatg accacatcgg
      241 tcttggtttc cttgagcaat tttgagattt cagcagtgga tcccggtgct tttttgatga
      301 cttcactcaa atatttgccg aggccgtcat gggtcatgcc tcgcaccact ggtacgccga
      361 ggtgggggac atcgcagaat ttgtaggtgt tattgggttc ggcgaagatg gcttctgaaa
      421 ggtccttgcc gaccttggtg gcattgatat ctattgccag tgtgaattca atatcacgga
      481 cgtggtattc gccaagctgg gtgtgcataa tgcctgggat ggtttcatcc tcttttgcgt
      541 tcttgtagaa ctgaacaccc tgcacaagcg aagaggcgca atttcccacg ccaatgatcg
      601 caacgcgtac ttttttgttt gccatggttg tttctccttt tcgttgttta attacaattt
      661 ccttaagcct gctgtttgcc tgccactaca gatagacctg tgaagtataa tcttactgta
      721 agatatgtag gtaatcatgg ccatggatca agtgacacta cttgcaagac tattggaagt
      781 tacacgcaac ctcagcacaa tggtggatgt ggatgcgtac ctgcaatcca ttctttcggc
      841 ggcaaccgag ttgactggca gcgaaaccgc ttccctgatg gaatacgacg aaaccacgca
      901 ggaattcttt ttcaggttcg taccttggtt tcaccgtgac ctgatcaagg atgcccgtgt
      961 cccattgaat gggagcgccg ctgggtggtc gcttttgcac tccaaacccc tgatcatcaa
     1021 tgacgtaaag aatgacgagc ggcacttcaa taaagtggat gagatggcga attttgtcac
     1081 ccgctccatg ttggtggtgc cgcttttact gcatggcagg ccgatggggg tcttcgaagt
     1141 catcaataaa gccaacgacg ctccatacac tgatgaagat atcctcgctt tggaaaccct
     1201 tgcttcactg gctactacag ccatacagaa caatctgctt gaaaacagca ttcaatcttc
     1261 ccaaaacgaa tcacgcgacc ttgaccgtct aaagaatgaa ttcatcgcga tcacctcgca
     1321 tgaactgcgt acaccgctgg gcttgatcct cggccatgcc acgtttttac gtgaattggt
     1381 ggggaatgat tttcaagaac aggtggaggc cattatccgc aatgcttcca ggttgaagga
     1441 gatcattgaa agcctctcca ctgtggataa ttaccaaaca ggcggtgcca gtctccgttc
     1501 gcgcagggtt tccatgaacc gcgtcattga agatgtcatt gcttcgttcc gcgatattgc
     1561 caagaagaag ggcgtttcat tgaagtcgga cctcgaccgc aatcaagagc tgctcgtgga
     1621 tgcagatggc agcaagatcg ccatcgtatt gagcaacctc gtcaagaatg ccgtcacctt
     1681 taccaatgat ggtggtcaca ttacagtacg cggcagcctt gactcgagtt ttgtaaaagt
     1741 agtcgttgaa gatgacggcg tgggcatccc tgcccgtgac ctgccgcatg tatttgagcg
     1801 tttttatcag gtcgaatctc atcttacgcg caggcatggc ggcatggggc tgggcctgtc
     1861 tgttgcaaaa gtgatgatcg aaatgcatgg cgggcgcatt tgggccgaaa gcctcgaagg
     1921 gcttggcagc aagttcacct tcctgctgcc gttgcacccc aaggtgaatg agcccgaccc
     1981 tgtttcacct ttcgttgaat aaaaaacgat ctctcgaatt tcccaatccc gtcaaatgag
     2041 ttgacgggat tttgattcct ttgatacact cgctccatta aatcgcaaag gaaaacccat
     2101 gcaaagcatt aatgagcaat tcgaattgat tcgccaatct gccactgttg ccatggcaga
     2161 ccgtattctg gcactcaaag ccagtggccg tgatattgtc ggtttgcaag tcggtgaccc
     2221 cgattttgga acacacccag ccattgccga agccgccatg aaagccattc aaagcggatt
     2281 gacgcattat ggcccttcac gcggttatcc tgagctgaga actgctgttg ccaataaact
     2341 gatgcgggat gaaggcgtta cttacgaccc tgccagtgag atcctgatta cacatggcgg
     2401 catccatgcc tattaccttg ccatgcaatc cattttgaat ccaggggatg atgtgctgat
     2461 tcctgacccg tcctgggcaa cccattcgaa catggcgatc atgctgcgcg gaaatgtcat
     2521 ccgcatccct gcccccgccg aaaatggatt catcccccat tttgaatcgt ggctgaaagc
     2581 gttgaccgcc agcgcgaaac cccgcgtcat cgttctcaac tatccctcca acccaacagg
     2641 ggcatatccc acacgcgact acctgcaaaa gctgcaagac tttgccaaag aacacagcct
     2701 gtggatcgtg agcgatgaag tgtatggcag tctgttctac gaaggacgtc cgacttctgc
     2761 agcagcgatc gaaggtgcaa aagaacgcac actgatcatc aacagccttt ccaaatccta
     2821 tgccatgacg ggttggcggg tgggattcct tgccgctcct gcccatatta ttgagaatgc
     2881 tctcaaggcg gggcagaatt ccattacttg tgtagccccc ttcattcaaa aagctgctgc
     2941 ctttgcgcta accgacccag ccatgcagga attcaccgaa gaaatgcgaa cgggctattc
     3001 ccgccgcagg gatttggtac tgcgcctgtc acatcagttg gaaagcgaga aagtaaaagt
     3061 gattcctccg cagggagcat tttatttttt ccttgatctg cgcgctttga atatgtcttc
     3121 tgtggaaatg tgtgaaaaga ttttggatga ggttggcgtg gggctggtgc ctgggtccgc
     3181 ttttggcgag cagggcgaag gttttatccg catgaccatt gccgcctccg atgcagatgt
     3241 ggaagcaggg tttaggaaga tcgtggaatg ggccgaaagg cagtaattgg agatgaacat
     3301 gaaagtatct tttcagggtg aacctggcgc gtattctgaa caggcggtct ttaattattt
     3361 tggcgaagtg gaaacggttc catgtgaatc atttgatgtg atgttcgatt cggtcgtttc
     3421 aggcaaaagc gatgtggctt tggctcccat tgaaaattca ctggcgggta gtattcacca
     3481 gaattacgac ctgttattgc ggcatgacct gcatattgtg ggggagtatt tcctgcgcgt
     3541 gcggcattgt ctgatcgcca tgcccggtgc gaagatggaa gatatcaaaa aagccatcag
     3601 ccatccacag gcgctgggtc aatgtgctgc gtatttgcgg ggcaggggca tcaaacctga
     3661 aaccgtgtac gatacagcag ggagcgtgaa aatgctcaag gcttcaggag ccatggatac
     3721 agccgccatt gcatcacgcc gtgcggctga attgtacgga atgcagatcc tggaagaggg
     3781 gatcgaagac aatagcgaaa actatacgcg tttcctcgct gtccaacgag aggcagcttt
     3841 cccgaagggc gaagcgaaga cttccatcgt gtttacattg aagcatgtac cgggatcgct
     3901 tttcaaggcg atgtctgttt ttgccttgcg cgatattgac ctgaccaaga tcgaatcacg
     3961 cccgttgcag ggcaaaccgt gggaatatct tttttacatt gatttcatcg gttccgccga
     4021 tgatgacacc ccaagacggg cgcttgagca tttgggtgag tacgccacca ccttgcgcgt
     4081 gttggggtcg tatccacgct ttaggggttg atcgttccca acgctctttc cagtttttga
     4141 aaggtactgc gccagccctc agcataatta tgcattgacg ttgaagggtc aaccttcgat
     4201 tgctcaaagg tcatgagcgt tccagcgccg tctggagaaa attccaatga aatcaattca
     4261 tgtacttcag gttggtttgc ccagccccac gtgaaaacga gacggttcgg ttcattgacc
     4321 tctttgtaaa ttccctgcgc tgtaaattca gcgccatcgg aattggcaaa ggtcaccgaa
     4381 aacgatccat tgacttgcac atcaagtttc gcatccagca ctttgccatt tgggtcagaa
     4441 ccgaaccata acttcgtgat ggtttggtca gtccatgcct gccagacttt gacgggtggg
     4501 tgagtaaact cccacgagat acggatggaa cgacttgatg gagtcgtaat ggacttaccc
     4561 tcctaaccgc tcgatcaaaa acaagacctc cgccgtatag ctataagaga gcgcaggatc
     4621 atctcccaac gctttatcac gagagccatt gagaatatca acaacaattt gcatgaactt
     4681 tttgaaataa tcaggtgcat ctgagtattg attcaaaaga ttctgaatct catccgattt
     4741 tccttgctgt atcaatccta atatctgatc tataaactgc ccacctgcgc cttggcttgc
     4801 atacccaccc tgccgacgat acgccaaata cgcatcgcgc gcctgctccc aggcggcacg
     4861 cgcggcggtg gagttgccag tcgattcttc gatgttatgc aaaatatcaa aggatttcca
     4921 tggctcagcc gcaagaccaa tttggctttt acactcaatc gcccgcatga tctcggcgcg
     4981 ggcttcgtcg tagcgtttga gttgttgcaa agtgttggcg atattatttc gagtaaatcc
     5041 ttcatagcgc aaatcaccta tttcaacgta aatatccgct gactgtctgt aaaagacgac
     5101 cgcttcttca gggcggttca acttgtcatt gtaaagtact cccaaattaa tcaaactatt
     5161 cgcctgccca gcccgatcat tattctgaga cttgatctcc agcgagcggc ggtaggcggc
     5221 ttcggcgctt tcgtaattgc ccgcctcctg atgcactatc ccgatctggt gccagataga
     5281 agcgatagac ttaggttcat gttgcttttc aaagataggc agggcttctt cataagtgtc
     5341 aattgcctct ttatatttct cctgcttac
//
LOCUS       DAOQJC010000197         5268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_9111_length_5268_cov_6.447727, whole genome shotgun sequence.
ACCESSION   DAOQJC010000197 DAOQJC010000000
VERSION     DAOQJC010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9111_length_5268_cov_6.447727"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PLE39_18375"
     CDS             complement(<1..761)
                     /locus_tag="PLE39_18375"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNE70356.1"
                     /translation="MELLWPGFLYLLALIPLLVLVYIFVLRRRKPYAVRYSSLSLVRE
                     ALGKQSQVRRHLPFVLFLLAMSSLVVAMGRPVSTITVPASNATIILAIDVSRSMCSTD
                     ISPNRLEAAKTAAWDFIAAQESNVQIGLVAFAGYAVVIQEPTTERSLLETAIQNITTA
                     RRTAIGSGLLKSLEALSEIDDSMPSPFAGTEPEPMPAGQYAPYIVVLLTDGVNTTGPE
                     PVFAAEFAKTRGVRVYTIGFGTAQEDAPMNCGGFQG"
     gene            complement(893..1909)
                     /locus_tag="PLE39_18380"
     CDS             complement(893..1909)
                     /locus_tag="PLE39_18380"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNE70357.1"
                     /translation="MSFLWPTLLFSLLAIPLLALLYLRMQNQRKQLAVRYGSLGLVQQ
                     TSGRAASSRRHLPAILFLIGLAILFFSLARPQMVASLPKVEGMVILAFDVSGSMTAAD
                     FEPTRMEAAKAVGRDFVQRQPETVKIGIVSFSDNGFSVQLPTNDKEAILASIDRLRPE
                     RGTSLANGIIISLNTIANVTGQPPILGMDVPQPDASSPAPTPAPVSSADDSAVIVLLT
                     DGENNMDPDPLEAAQFAADRGVRIHTIGVGSSAGVQLEVNGFSVFTQLDEATLRQIAE
                     ITDGNYYNAQTEEDLQTIYENIDPQLVIVEEKTEVTAIFAGASILILLVAGMLSLLWF
                     SHVP"
     gene            complement(1912..2811)
                     /locus_tag="PLE39_18385"
     CDS             complement(1912..2811)
                     /locus_tag="PLE39_18385"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNE70358.1"
                     /translation="MNTSTSTPTPERILHRLDWQVIRRLDGLLQGDYRSLFRGFGVDF
                     ADLREYQPEDDIRYIDWNVSARMNTPYVRQYMEDREVIAWFLLDLSPSVDFGTLQNQK
                     RNVLIDFVATLARLLTRHGNRVGAMMFGSAIQRTVPARSGRLQVLRIINDLLNQPRLP
                     HSSMTDLSALLKGGLHSIKKRSLVFVVSDFISTPGWERTLNLLSQHHEVIAIRLWDPR
                     EMDLPDIGPIVMEDAETGEQLYVDTHDKKFRERFRAAAQQREIALKDSFKRAGVDVLP
                     LSTEEDLIHAIARFVMMRRQHKR"
     gene            complement(2780..3790)
                     /locus_tag="PLE39_18390"
     CDS             complement(2780..3790)
                     /locus_tag="PLE39_18390"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNE70359.1"
                     /translation="MEQTSNRENKLPMERVLYEVKRVIVGQDHLLERMIVALLARGHI
                     LVEGVPGLAKTMAIKTLADSIGGEFKRIQFTPDLVPADLIGTRIYNQKSGEFNTSLGP
                     VFTNLLLADEINRAPAKVQSALLEVMQEKQVTIGRETHKVPNPFLVLATQNPIETEGT
                     YALPEAQVDRFMLKVLVGYPSPTEEFVIVERMTGAWTEVQKVLTTDQLVELQKKTDQV
                     YVDPALIEYAVKMVNATRNPKEVGLGEVQRYIMFGASPRASINLILTAKALAFVRGRS
                     YVLPQDVLDMALDVIRHRLVLSYEALSDNISSDDILKKILERIPLPVVPLHEHFNVHA
                     NA"
     gene            complement(3898..4737)
                     /locus_tag="PLE39_18395"
     CDS             complement(3898..4737)
                     /locus_tag="PLE39_18395"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNE70360.1"
                     /translation="MNESSASKMDKWKGRMREVIARLRRGLPFASGVFGALLVLFFYN
                     LLVVKPNQLTVEDVNASVAMVMASATPAPSYSTQVYQAIRPSLILIQVEEKHQNAESD
                     FGLGSGVVVDSFGNILTSLHVVDGASVIMVTFADGTQSEAAIVSEMPERDIAVLQAYD
                     TPLSVVPAVLGNPNSMRVGDEAFVVGNPFGLYSSMSSGVISGFDRVFQMEGTGLEIPG
                     MIQIDAAVNPGNSGGPLLNRNGHVIGIVTGIINPTDESFFVGIGFAVPITVAVGGMGS
                     PPY"
     gene            5018..>5268
                     /locus_tag="PLE39_18400"
     CDS             5018..>5268
                     /locus_tag="PLE39_18400"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE70361.1"
                     /translation="MEGGTPSIPIWFDKDDLKAGMDWDIQIDDALKSCRMVLFVMTKD
                     STHDKSVCRQEWSRALSFKKPIIPLWVENNIDVPFRLADR"
BASE COUNT         1269 a         1435 c         1413 g         1151 t
ORIGIN      
        1 ccttgaaagc cgccgcaatt catgggggca tcttcctgag cggttccaaa tccgatcgta
       61 taaactcgga ccccgcgggt cttggcaaac tccgccgcga agacgggctc tggcccagtg
      121 gtgttgacgc cgtccgtcaa caggacaacg atgtacggcg catattgccc tgcaggcatg
      181 ggctctggct ctgtgcctgc gaacggactt ggcatactgt catcaatttc agaaagcgct
      241 tcaagagatt tgagcaagcc cgagccgatg gctgtcctgc gggcggtggt gatgttttga
      301 atggcggttt caagcagact gcgttcggtg gttggctcct gaatgacgac cgcatacccc
      361 gcaaatgcca ccagcccaat ttgcacgttc gattcctgcg cggcgataaa atcccatgcc
      421 gctgttttgg ctgcttcgag gcggttcgga gagatatctg tcgagcacat gcttctggaa
      481 acatcaatgg caaggatgat agtggcattt gaagcgggca cagtgatcgt ggaaacgggc
      541 cgccccatcg ccaccaccag gctcgacatt gccagcaaaa acaacacaaa cggcagatga
      601 cgccgcacct gtgattgttt tccaagcgcc tcacgcacca aagacaggct tgagtaacgc
      661 accgcatacg gttttcgtcg gcgaagaaca aagatgtaaa ccagcaccaa caaggggatc
      721 agtgccagca aataaaggaa tccaggccag agcaattcca tgtcagcctc caatctcacc
      781 acagagcaca cagagaccac ggagaatttt aagaggcttt tctctgtgga ctccgtgatc
      841 tctgtggtga aaaaatagaa aactaaacca aataccagag ggtctaaaaa agctatggca
      901 catggctaaa ccacaacagg gagagcatcc ctgcaaccaa taaaatgaga atgctcgcac
      961 ccgcaaaaat ggcggtgact tccgtctttt cctccacgat caccaattgc ggatcaatat
     1021 tttcgtagat ggtttgcaga tcttcctcgg tttgggcatt gtaataattt ccgtctgtaa
     1081 tctcggcgat ctgtcgcaac gtggcttcgt ccaactgcgt gaagaccgaa aagccattca
     1141 cctccagttg aacgcctgcg ctgctgccaa ccccaatggt gtggatgcgc acgccgcggt
     1201 ccgcggcgaa ttgagcggct tcgagcgggt ctgggtccat gttgttttcg ccatcggtga
     1261 gcaacacgat cacagcggaa tcatctgcac ttgaaacagg agcaggcgtg ggcgcaggag
     1321 aagaagcgtc tggttggggg acatccatgc cgaggatggg aggttgtcct gttacgttgg
     1381 caatcgtatt taacgagatg atgatcccat tggcaagcga agtaccgcgc tcggggcgca
     1441 aacggtcaat ggaagcgagg atggcttcct tgtcgttggt gggcaattgc accgaaaaac
     1501 cgttgtcgct gaaagagaca atgccgatct tcaccgtctc tggctggcgt tgtacaaaat
     1561 cacgccccac cgctttggcg gcctccatgc gggtcggctc gaaatcggcg gcggtcatac
     1621 tgcctgaaac atcaaaagca agtatgacca tgccttccac cttcggcagg cttgccacca
     1681 tttgagggcg cgccaatgaa aaaaacaaaa tagccaggcc aatcaaaaac aaaattgcag
     1741 gaaggtggcg gcgcgaactg gcagcgcgtc cgctggtttg ctgcaccagg cccaaactgc
     1801 cataacgcac agccagttgt ttgcgctggt tttgcatgcg gagatacaac aaagccagca
     1861 gcggaatcgc cagcaaagaa aaaagcaacg tgggccagag aaaagacatg gttacctttt
     1921 gtgttgacgc cgcatcatca cgaaacgcgc aatggcgtgg atcagatctt cctctgtgga
     1981 aagcggaagg acatcgaccc ctgcgcgctt gaaagaatct ttcaacgcga tttcacgctg
     2041 ttgcgcggcg gcgcggaaac gttcgcggaa ttttttatcg tgcgtatcca cgtataactg
     2101 ctcgcccgtt tctgcatctt ccatcacgat cgggccgatg tcgggcaggt ccatttcgcg
     2161 cgggtcccac aagcggatgg caatgacttc gtgatgctgg ctcaacagat tcaacgtgcg
     2221 ctcccagcca ggcgtgctga tgaaatcaga caccacaaaa acgagcgagc gttttttgat
     2281 ggaatgcaat ccgcctttga gcaatgcgct cagatcggtc atggaagaat gcggaagacg
     2341 cggctgattc aacaggtcgt tgatgatgcg cagcacctgc agccgcccgc ttcttgctgg
     2401 gaccgtgcgc tggattgcgc tcccaaacat catcgccccg acgcgattcc cgtgacgggt
     2461 caacagcctt gcaagcgtgg cgacaaaatc aatcagcaca ttgcgtttct ggttttgcag
     2521 cgtgccaaaa tccaccgatg gactcaggtc gagcaaaaac cacgcaatga cttcgcggtc
     2581 ttccatgtat tgacggacgt acggcgtgtt catgcgcgcg ctgacgttcc aatcaatgta
     2641 gcggatgtca tcttcaggct ggtattcgcg caggtcggca aaatccacgc cgaagccgcg
     2701 gaagagactg cggtaatcgc cctgcagaag tccatccaac ctgcggatga cctgccaatc
     2761 gagccgatgc agaatgcgct caggcgttgg cgtggacgtt gaagtgttca tgcagtggca
     2821 ccacagggag cggaatgcgc tcgaggatct tcttcaaaat atcgtcgctg ctgatgttgt
     2881 cggaaagcgc ttcgtacgaa agcaccagcc tgtggcggat gacatccagc gccatgtcga
     2941 gcacatcctg cgggaggacg tagctgcgtc cgcgcacgaa ggcgagcgcc ttggcggtga
     3001 ggatcaaatt gatggatgcg cgcggactgg ctccgaacat gatgtagcgt tgaacctcgc
     3061 ccaggcccac ctcttttgga ttgcgcgtgg cgttgaccat tttgaccgcg tattcaatga
     3121 gggctgggtc cacatacacc tgatcggtct tcttttgcaa ttccaccaac tggtcggtgg
     3181 tcagcacttt ttgcacttcc gtccacgcgc ctgtcattcg ttcgacgatg acaaattctt
     3241 cggtggggct ggggtagccc accaacacct tgagcatgaa gcggtccact tgcgcttcgg
     3301 ggagtgcgta cgtgccttcg gtttcgatcg ggttttgagt ggccagcacc aaaaacggat
     3361 tcggcacttt gtgggtctcg cggccaatgg tgacctgttt ttcctgcatc acttcgagca
     3421 gggcgctttg cactttggcg ggcgcgcggt tgatctcatc ggccaacaat aaattcgtga
     3481 acacaggccc aagcgaggtg ttgaactcgc cgctcttctg gttgtaaatg cgcgtgccga
     3541 tcagatccgc ggggacgagg tctggcgtga attgaatgcg cttgaattcg ccgccgatgg
     3601 aatcggcgag ggtcttgatc gccatcgtct ttgccaggcc tggcacgcct tccacgagga
     3661 tatgtccgcg ggcgagcagg gccacgatca tgcgctcgag caaatggtcc tgccccacga
     3721 tgactcgctt cacttcgtac aacacgcgtt ccattgggag tttgttttca cggtttgagg
     3781 tttgttccat gcctgtgctc cttttgtctc tcagtaaata tgtaggggcg acccttcaat
     3841 agggttgaag tcaactgtct tccagaggcg ggtcgcccct acagtccaga aaatgaatta
     3901 atacggcggc gagcccatgc cgcccaccgc cacggtgatc ggcaccgcaa acccgatgcc
     3961 gacgaaaaaa ctttcatcgg tggggttgat gatgccagtg acaatgccaa tgacatgacc
     4021 atttcgattc aacagaggcc cgcccgagtt cccagggttg accgctgcgt caatttggat
     4081 catgcctggg atctccaaac cagtcccttc catctgaaag actcggtcaa agccagagat
     4141 cacgcccgag ctcatcgagc tgtacaaacc aaaggggttg cccactacaa atgcctcatc
     4201 ccccacccgc atcgaattgg ggttgcccaa cacggcaggc accaccgaaa gcggcgtatc
     4261 gtacgcctgc agcaccgcaa tatcacgctc aggcatttca gagacgatcg ccgcctctga
     4321 ctgcgtgcca tcggcaaagg tgaccatgat cacgctggcc ccatccacca catgcagact
     4381 ggtgagaatg ttgccgaagg aatcgaccac cacgccgctt cccaacccga aatctgactc
     4441 agcgttctga tgcttctctt ccacctgaat caggatcagc gacggccgaa tggcctgata
     4501 cacctgcgtg gaataggacg gggcaggcgt cgccgaagcc atgaccatcg ccaccgaagc
     4561 gttgacatct tccacggtca gttgatttgg ttttacaaca agcaggttgt agaaaaacag
     4621 gaccagcaaa gcaccaaaga cccccgaggc aaaaggcagc ccgcgccgca aacgcgcaat
     4681 gacctccctc atgcgacctt tccatttatc catttttgaa gcagacgatt cgttcatgca
     4741 cagttcctga tcaagtaagc tggcgtacac gatatttgcc aatataagga ctttatttta
     4801 gataaagatg agaaaagttg tcacattctt acagatttac aaaacccaca taattacacc
     4861 cccaagtgcc gattttcaat acggggtggg tataattgcc gcatcacatt caaactgtaa
     4921 gcgattcgaa acatgagggc tttccatgaa cggtcattgc ttcatcagtt attccaccgc
     4981 agatgctttg gagttcgcgc gtaaacttgc aaacaaattg gagggcggaa ctccaagtat
     5041 tccaatctgg tttgataaag atgatttaaa agcaggcatg gactgggata tccaaattga
     5101 cgatgccttg aaatcatgca gaatggtgtt gttcgtgatg accaaggaca gcacgcacga
     5161 taagtcggtt tgcaggcagg agtggagtcg cgcgcttagt ttcaaaaagc caattatccc
     5221 cttgtgggtt gaaaataata ttgatgtacc tttccgcctt gccgaccg
//
LOCUS       DAOQJC010000198         5216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_9255_length_5216_cov_9.365433, whole genome shotgun sequence.
ACCESSION   DAOQJC010000198 DAOQJC010000000
VERSION     DAOQJC010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9255_length_5216_cov_9.365433"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1782)
                     /locus_tag="PLE39_18405"
     CDS             complement(<1..1782)
                     /locus_tag="PLE39_18405"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE70362.1"
                     /translation="MGFFAGLNDEKYDRQYSDRELTRRIAGFFKTKARQLITASVLVI
                     TLAFIGAALPVVVSRMVDLLREQPTIQEIVLVGVTLMGVGVGLWGLNWMRRSLIVRAV
                     GDVVLDLRTRAFRAAAEHDLSFYDQFSSGRIVSRITSDTNDFGQLIVIITDVVSQLMQ
                     AFILGVVLFRTELKLSLLLIAFMPMIFAVAAGFRALARRVTKRGMKAMADVNAAIKET
                     ISGISIAKNFRQEQSIFASFDESNQQSYQVNVRRGFVLALVFPTLNALSGVFVGILIY
                     VGGMSAEQGLVSIGAWYLFIMSLDQFFFPILNLTSFWAQIQGGLSAAERVFALIDADP
                     NVIQKEKQDVPRLKGEIRFENFDFSYSDKEPILRNFNLAIQPGETLALVGHTGAGKSS
                     IAKLIARFYEYQNGELRIDGRDIRTFDLTQYRRQLGIVSQVPFLFSGTVIDNIRYAAP
                     GVPESEMLKLAKKIGDGEWLETLPDGLHTEVGERGNRLSMGQRQLVALMRVLVQNPAI
                     FILDEATASIDPFTEWQIQQALNLILKNTTSILIAHRLSTVKAADRIVVMQKGSIIEQ
                     GDHDGLLKQGGHYAELYNTYFRHQSLSY"
     gene            complement(1888..3651)
                     /locus_tag="PLE39_18410"
     CDS             complement(1888..3651)
                     /locus_tag="PLE39_18410"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE70363.1"
                     /translation="MSSISLPAEFTLHRKNSYNRATTLKWIASHARPYWWIVIMIIIG
                     AIGNAALAAYVPVLTGDAFNSMIESPPDTSVLLPLALIIGISQIVRGVLQLGRNFGAE
                     LMAQRMERDIRDELYLSLLGKSMTFHNLQPVGDTMARATNDVREVNFMFNPGVNLVVG
                     SFMFIIMLFFAAPRYHPSLLITPTIFTIIYFIGLARYLKSLAPITDEVRATFGDMNTH
                     LSESLDGVEIMKGAAQEGAEVDRFVSNARKVRDAFVRQGDLEGRYIAMLLLGTAYAGG
                     LVHALLLFRAGTIDLGAVVAYFGLLRLLEFPTFTSTWAYSQISSGLSSARRILELINR
                     ETHLDQNAAGRATEMRGEVEFRGVSFAYPEGDAVIQDVSFKIKQGQTVAIVGQTGAGK
                     TSLARLINRTYDVTAGQVLIDGVDVREWNLAALRSQISIIEQDIFLFSRSLSDNIAFG
                     KPGATKEEIISASIASQAHDFILDFDQTYGTVVGERGVTLSGGQRQRIALARAFLTDP
                     RILVLDDSTSAIDSATEDKIQRAIANAARGRTTFIITHRLSQIRWADLIIVLRKGKIA
                     AIGSHDELMKTSEAYSRIFRE"
     gene            3764..3913
                     /locus_tag="PLE39_18415"
     CDS             3764..3913
                     /locus_tag="PLE39_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70364.1"
                     /translation="MKMPKTISGWCTILFFLFTGLGAFVAIPLLPTLVGISALGVVVF
                     TLIGK"
     gene            complement(4031..5011)
                     /gene="pyrB"
                     /locus_tag="PLE39_18420"
     CDS             complement(4031..5011)
                     /gene="pyrB"
                     /locus_tag="PLE39_18420"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNE70365.1"
                     /translation="MPTQSSSPHLPFGENKQADWYGKDILSVKQFSRDDLEYVFGVAH
                     EMRGMVERVGTFDLLKGKILANLFYEPSTRTSSSFTAAMERLGGSVIPISEVKYSSVS
                     KGESLPDTIRTLECYADVIVLRHPETGSAAIAAKAARKPVINAGDGVGEHPTQALLDT
                     FTIMEEIGRLDNLTVTMLGDLKYGRTVHSLARLLSTFTNIKLNYVSPDILKMPKEVME
                     EVGAKNIPQAEFSSLEKVLPETDVLYVTRVQKERFEDPADYEKVKGAYVIDPEIMKAA
                     KKDMIVMHPLPRVGEISTDFDDDPRAAYFRQMEYGLYVRMALLALVLGKA"
BASE COUNT         1244 a         1363 c         1394 g         1215 t
ORIGIN      
        1 ataagaaaga ctctgatgcc tgaaataagt attgtacaac tcggcgtaat ggccgccttg
       61 cttcaataag ccatcgtggt cgccctgctc aataatggaa cctttctgca tgacgacgat
      121 acggtctgcg gctttgacgg tggagagacg atgagcgatg aggatactgg tggtgttttt
      181 gaggatcaaa ttcaatgcct gctggatctg ccattcagtg aatggatcaa tgctggcagt
      241 ggcttcatca agaatgaaaa tggcggggtt ttgcacgagc acacgcatca acgccaccag
      301 ttgacgctgc cccatggaaa ggcggttgcc gcgttcaccc acttcggtgt gcaggccgtc
      361 gggcagggtt tcgagccatt caccgtcacc gatctttttg gcgagtttga gcatttcgct
      421 ttcggggacg cccggcgcgg cgtagcggat gttgtcaatc actgtgcctg aaaacaggaa
      481 agggacttgc gaaacgattc ccaactgacg gcggtattgg gtcaggtcaa aggtgcgaat
      541 gtcacggcca tcgatgcgca gttctccgtt ttggtattcg taaaaacgcg cgatcaattt
      601 tgcaatggat gatttacccg cgccggtgtg ccctaccagg gcgagagtct ctccgggctg
      661 tatggccaga ttgaagttac gcaggatcgg ttctttatcc gaatagctga aatcaaaatt
      721 ttcaaatcgg atctcgcctt tgaggcgcgg cacatcctgc ttttcctttt ggatcacgtt
      781 cgggtcggca tcgatcaagg caaagacacg ttcggcagcc gaaaggccgc cctggatttg
      841 cgcccaaaac gatgtgagat tcaagatcgg gaaaaagaac tggtcaaggc tcatgatgaa
      901 gagataccat gcgccgatgc tcaccagtcc ctgctccgcg ctcatgccgc cgacataaat
      961 gagaatgccg acgaaaacac cagacaaggc attgagcgtt gggaaaacca gcgccagcac
     1021 aaacccgcgc cgcacgttga cctgatacga ctgctgattc gattcatcga atgaagcaaa
     1081 gatgctttgc tcctgcctga aattctttgc aatggaaatg ccgctgatgg tttccttgat
     1141 cgcggcgttg acatctgcca tggctttcat gccgcgtttg gtcacacggc gggccagtgc
     1201 gcgaaaccct gccgccaccg caaagatcat gggcatgaat gcgatcaaaa gcaaagacag
     1261 tttcaactcg gtgcgaaaca aaaccacacc aagaataaaa gcctgcatca actgcgagac
     1321 cacatcggtg atgatgacaa taagctggcc gaaatcattc gtatcggatg tgatgcgcga
     1381 gacaatgcgc cccgatgaaa attgatcgta aaacgagagg tcgtgttcgg cggcggcacg
     1441 gaaggcgcgt gtacgcaggt cgagaacgac atcgcccacg gcgcgcacga tcagactccg
     1501 ccgcatccag ttcagtcccc acaagccgac tccaaccccc atcagtgtga ctcccaccag
     1561 gacgatctcc tgaatcgtcg gctgctcacg gagcaggtcc accatacggc tgaccaccac
     1621 tggcaatgcc gcgccgatga aggccagtgt aatgaccaat accgaggcgg taattagttg
     1681 tctggctttg gttttgaaaa accctgcaat gcgacgcgtg agttcacggt cgctgtattg
     1741 tctatcgtat ttttcgtcgt ttagtccggc aaagaaaccc atttttgatt tccgattttt
     1801 tgatattagg cttgtgatta atggagtgcg gacttcagtc cgcttttcta caggagtgga
     1861 ctaaagtccg cattcctggg ttgggaatta ttctctgaaa atccttgagt acgcttcgga
     1921 ggtcttcatg agttcatcgt gtgaaccgat ggcagcgatc ttgcccttgc gcaggacgat
     1981 gatgagatcg gcccagcgga tctgcgacag gcggtgcgtg atgatgaagg tcgtccgtcc
     2041 gcgggcggca ttggcgatgg cgcgttggat tttatcttcg gtggctgagt cgatcgcgga
     2101 tgtggaatca tcgagcacga gaatgcgcgg gtcggtgaga aaggcccggg caagtgcgat
     2161 gcgttgacgt tgtccgccgg agagggtcac gccgcgttcc ccgacgacgg ttccgtaggt
     2221 ctggtcaaag tccagaataa aatcatgcgc ctgcgacgcg atggaagctg aaatgatctc
     2281 ttccttggtg gcgccaggtt tgccaaaggc gatgttatcg ctcagcgaac gtgagaataa
     2341 aaagatatcc tgctcgatga tggaaatctg cgaacgcaaa gcagccagat tccattcgcg
     2401 cacatccacg ccatcgatca agacctgacc cgctgtgaca tcgtaagtgc ggttgatcag
     2461 cctcgcaagc gaggttttcc ctgcacccgt ctgccccaca atggcgacgg tttgtccctg
     2521 cttgatcttg aatgagacgt cttgaatgac agcgtcgcct tcagggtagg caaaggaaac
     2581 gccgcggaat tccacttctc cgcgcatttc ggttgcgcga cccgcggcat tttggtcaag
     2641 atgtgtttcg cggttgatga gttccaaaat acgacgcgcg ctggaaagcc cggaggagat
     2701 ctgtgaatag gcccaggtgg atgtaaaggt ggggaattca agtaaacgca acagaccaaa
     2761 atacgcaacg actgcgccaa ggtcgatcgt ccctgcacgg aaaagcagca acgcgtggac
     2821 aagtccgcct gcgtaggcgg tgcccaaaag cagcatggca atgtagcgtc cttcgaggtc
     2881 accctgacgg acgaaggcat cgcgcacctt gcgcgcattg gacacaaaac ggtccacttc
     2941 tgcgccttcc tgcgcggcgc ctttcatgat ctccacaccg tccagcgatt cggagagatg
     3001 tgtgttcatg tctccgaagg tggcgcggac ctcatccgtg atcggtgcca gcgacttgag
     3061 ataacgcgcc agtccgatga aataaatgat cgtgaagatc gtcggcgtaa taagcaggga
     3121 agggtgatac cgcggcgcgg caaagaacag catgatgatg aacatgaagg agcccaccac
     3181 caggttcacg ccggggttga acataaaatt aacctcgcgc acatcgttgg tggcgcgcgc
     3241 catcgtatca ccgacgggtt gaaggttatg gaaggtcata ctcttgccaa gcagggagag
     3301 gtataactcg tcgcgaatat cacgttccat gcgctgggcc atgagttcgg cgccgaagtt
     3361 gcgtcccaac tgcagcacac cgcggacgat ctgcgaaatg ccgatgatca aagccaacgg
     3421 cagcaaaacc gatgtatccg gcggtgattc gatcattgaa ttgaaggcat cccctgtcag
     3481 cacaggcacg taggcagcca atgcagcgtt gccgatcgct ccaatgatga tcatgatcac
     3541 gatccaccaa taagggcggg catgcgaggc gatccatttg agggtggtcg cccgattata
     3601 ggaattcttg cgatgcaggg taaattctgc gggaagggaa atggaagaca tgggttgatt
     3661 gtaccatttt gaaataaatg gcttttcgaa ccctttaatt cagacaggtg ttgtcttatt
     3721 agagtgacaa tcctgtcacc acacaacaca aggagaattt aatatgaaaa tgcccaaaac
     3781 gatctctggc tggtgtacga tcctgttctt tctttttacg ggtctgggag cattcgttgc
     3841 catccccctt cttcccacgc ttgtcggcat ttccgcactt ggcgtggtcg ttttcacatt
     3901 gatcggaaaa tagcccaatt ttctttttat cgttaacagg ttcgcaggtt ggaaagttga
     3961 aacgttaaac aactggaagg ttggcagcaa tcaaacctgc caaccttcca acttgtaact
     4021 taaaccgtac ttacgccttc cccaacacca gcgccaacaa agccattctc acatacaacc
     4081 catattccat ctgacggaaa tatgcagcgc gtggatcatc gtcaaagtct gtgctgatct
     4141 cacccacgcg tggcaacggg tgcatcacga tcatgtcttt ctttgccgcc ttcatgatct
     4201 cggggtcgat cacatatgcg cctttcacct tttcataatc tgcggggtct tcgaaacgtt
     4261 ccttttgcac acgggtcaca tagagcacgt cggtttcggg gaggactttt tccagtgacg
     4321 aaaactcagc ttgcgggatg tttttcgcgc cgacttcctc catcacttcc ttcggcatct
     4381 tcaaaatatc aggcgagaca taattcaatt tgatattcgt gaaggtcgaa agcaaccgtg
     4441 ccagcgagtg gaccgtgcgt ccatacttca gatcgccgag catggttacg gtcaggttat
     4501 cgaggcggcc aatctcttcc atgatggtga aggtatcgag cagcgcctga gtcggatgct
     4561 cgccaacgcc atcgcccgcg ttgatgacag gcttcctggc ggccttcgcc gcaatcgccg
     4621 ccgagcctgt ttcagggtgg cgcagaacga tcacatcggc ataacattcc aaagtgcgga
     4681 tcgtatcggg caggctctca ccctttgaca cggacgaata cttcacctcg ctgatcggaa
     4741 tgaccgaccc gccaagacgc tccatcgccg cggtgaacga tgacgaagta cgcgtggagg
     4801 gttcataaaa tagattcgct aaaatctttc ccttcaacag atcgaacgtc ccaacacgtt
     4861 cgaccattcc gcgcatttca tgtgccacgc cgaacacata ctccagatcg tcgcggctga
     4921 attgtttgac ggataaaatg tccttgccat accagtcggc ttgtttgttt tcgccgaaag
     4981 gcaggtgagg ggatgatgat tgagttggca tttgtttagt ctcctggtct tttagtttgc
     5041 tagtcttcca gtccttctgc ttcgaccaga taaccagccg accagaagac tattagacta
     5101 attttttaat cgggaatctg gttgcacact gacaacggct cccgttttat cacattttct
     5161 acgcctcggc aaaaatcccc gcgactcatt ccacgggtga ggctggttct gtttcg
//
LOCUS       DAOQJC010000199         5166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_9398_length_5166_cov_10.101937, whole genome shotgun sequence.
ACCESSION   DAOQJC010000199 DAOQJC010000000
VERSION     DAOQJC010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9398_length_5166_cov_10.101937"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PLE39_18425"
     CDS             complement(<1..906)
                     /locus_tag="PLE39_18425"
                     /inference="COORDINATES: protein motif:HMM:NF022307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HNE70366.1"
                     /translation="MTQQGFLMISDITGYSRYINESELEHARDSLTALLNLLIEHTQS
                     PLVLSKLEGDAVFSYAPAGGFLQRQSLLDMIETTYASFRKALELMALNTTCTCNACRN
                     LPNLDLKFFVHFGDYMTQKLGTFTELVGNDVNLVHRLAKNHIKEKTGFKAYAAFTQSV
                     MDVLGLVDFQNSLTPHRETFADVGEVQMYVLDMHSVWERLKDRVRIEVKPEDALVVRE
                     YIFPVPLSICWEYMTKPEFRQMVLGVEKVEVGNLKQGRSAVNSVYYCYHGNMETRLLI
                     LDWQPFEGYTTDGVTPIGASFSMTIK"
     gene            complement(938..2050)
                     /locus_tag="PLE39_18430"
     CDS             complement(938..2050)
                     /locus_tag="PLE39_18430"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNE70367.1"
                     /translation="MNINPNATMRFPLRFARGLNALIFSMLLLVQSACANNLTAAPTT
                     HPKEGTPVIDTPTPKPSKTPSPSPTTTSTPSIIGAWSDAPAMAIPRSAHAVANSDSAI
                     YALAGTDDHGKPVFEVEVFDGKDWKTETTLPGEGLNAPTASVVDQKLYVVGGFLAVSS
                     SPTNEVQVYDLQTQQWSNASPLPNPRGGHVAVVLDGRIHVFGGGNSVSTLADHSEYDP
                     ATDSWRDLAPLPRSEGSPAAVAIDGKIYVIGGRSGFSDFGDVYIYDPETDTWSTGPSI
                     DPRGTAGAVYYCGGIYLFGGESQSQRKNLDEAWRLDLERNVWEPVTAMPIARKFARAV
                     VFMNSVFIVGGSVVPASSHSPIGSNSVLKFTQPGCS"
     gene            complement(2175..2591)
                     /locus_tag="PLE39_18435"
     CDS             complement(2175..2591)
                     /locus_tag="PLE39_18435"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNE70368.1"
                     /translation="MGEVNDYNQRIINEFRANEGKVGGPFEGKTLLLLHTIGAKSQKE
                     RINPVACISDGERLVVIASKSGAPTHPDWYHNVVANPLVTVEVGMETFQAKASVAEEP
                     ERSRLYDKMVEVMPGFDVYRRKASRVIPVIVLERMD"
     gene            2820..3431
                     /locus_tag="PLE39_18440"
     CDS             2820..3431
                     /locus_tag="PLE39_18440"
                     /inference="COORDINATES: protein motif:HMM:NF022307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HNE70369.1"
                     /translation="MERKTQTGYLVLADISGYTSFVAKTEIEHAEKAISILLGNIIER
                     LDDLLSIVKLEGDAVFAYVSDEQVPDCNIMLELLDKTYEIFRDSARSLHAHATCPCKA
                     CRAIPELDLKFLVHHGEYIVQQVAGIKDLMGNDVTLIHRLAKNSVSESTGWNGYVLFT
                     GNCLERMQADKRPFIQQAETYEHLGNVETYVMNMHVRYDEMKK"
     gene            3705..3848
                     /locus_tag="PLE39_18445"
     CDS             3705..3848
                     /locus_tag="PLE39_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70370.1"
                     /translation="MSETTTKIKAKRPSKGMAKHNRLVKQEARKNSVPGTTVKKGKRP
                     EVK"
     gene            complement(3948..>5166)
                     /locus_tag="PLE39_18450"
     CDS             complement(3948..>5166)
                     /locus_tag="PLE39_18450"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNE70371.1"
                     /translation="ELLGAKIDSDKVPVYPSSKYRMTDMGAGDPAWIEEAAELLANSK
                     KPYLHAGKGVLWADAAPEFLELGNYLAAGMGSSMGARGAVPEDHPHYFFLFDMQATTL
                     ARNEADVVLVVGSRLGEYDGWGSHPAWGMPEKQKTIQIDVDANVIGLNRPVDVGILAD
                     AKSALKAILSKVKEKTAARSEMPDLARYRELSQQTMANGFQFLMAQPTRGLNPGQMVF
                     NARSFFPRNAVTVLDGGNTTLWGVAFNQIYEPRSFLYSVKMGYLGTGIPFAIGAKLAA
                     PGRPVYCITGDGAAGFNIMEMETAVRENAQIIVLVAVDDGWGMERSAHNFGGVPAELQ
                     QGVNISTEMRYDLIAQGLGCYAEKVDSVDVLPAALQRAVDSGKPALLHVTVDSAINAD
                     PPGYKQFRYVRTL"
BASE COUNT         1334 a         1378 c         1253 g         1201 t
ORIGIN      
        1 cttgatcgtc atgctaaagc tcgcgccgat aggcgtcacg ccgtctgttg tatatccctc
       61 aaagggttgc cagtccaaaa taagcaggcg ggtttccatg ttgccatgat agcagtagta
      121 aaccgaattg accgctgaac gtccctgttt caggttgcct acttccactt tttctactcc
      181 aagcaccatt tgacggaatt cgggctttgt catgtattcc caacagatgg acaacggcac
      241 agggaaaata tactcgcgaa cgaccaacgc atcttcgggc ttgacctcga tcctgactct
      301 gtccttcaag cgttcccaca ctgaatgcat atcaagcacg tacatttgca cctcccccac
      361 atccgcaaaa gtttcacgat gcggggtcag gctgttttgg aaatctacaa gtcccaacac
      421 gtccatcacc gactgggtaa acgcagcgta cgccttgaag cctgttttct ctttgatgtg
      481 attctttgcc agccgatgca ccaaattgac atcattcccc accagctccg tgaatgttcc
      541 cagcttctgg gtcatgtaat caccaaaatg gacgaagaac ttcaagtcaa ggttgggcag
      601 gtttcgacag gcattgcaag tgcaggtggt gttgagcgcc atcagttcca acgctttgcg
      661 gaacgacgca tacgtggttt cgatcatatc cagcaggctt tgtctctgca ggaaaccgcc
      721 tgctggcgca tacgaaaaaa cagcatcccc ctccagtttg gataacacca atggagattg
      781 tgtatgttcg atcaatagat tcaacaaggc tgtcaggcta tcgcgcgcgt gttcaagctc
      841 cgactcgttg atataccgcg agtagcctgt aatatcagag atcatcaaaa agccttgttg
      901 cgtcatgtca tggtttatat cacaggttag ttgaaattta cgaacaacct ggttgtgtaa
      961 acttcagcac agagttgctt ccgatcggcg aatgactgct tgcaggcaca acgctgccgc
     1021 caacgataaa aacagaattc ataaaaacca cggcacgggc aaattttcgt gcaatgggca
     1081 ttgcggtcac gggttcccaa acatttcttt ccagatccaa acgccaggct tcatccagat
     1141 ttttcctctg cgattgcgac tcgccgccga acaaataaat gcccccgcaa taatagaccg
     1201 ctcctgccgt tccgcgcgga tcaatggacg gccctgttga ccatgtgtct gtttcggggt
     1261 cgtaaatgta cacatcgcca aaatcggaaa atccgcttct gccgccaatg acgtagattt
     1321 tcccatcaat ggcgaccgcc gccgggctgc cttctgagcg cggcaatgga gcaaggtcac
     1381 gccaggagtc tgtggcgggg tcatattcgg aatgatccgc aagtgtggaa acggaattcc
     1441 ctccgccaaa gacatggatc ctgccatcaa gcacaactgc cacatgtccg ccgcgcgggt
     1501 ttggaagcgg actggcatta ctccattgct gggtttgtaa atcatacacc tggacttcat
     1561 tggtggggct gctggacact gctaaaaagc cgccgaccac atataacttt tggtccacaa
     1621 ctgaagccgt gggggcattc agtccttcac cgggcagggt cgtttccgtt ttccaatcct
     1681 tgccatcgaa cacttcaact tcgaataccg gcttgccgtg gtcatcggtc ccagccaatg
     1741 cataaatcgc cgagtcggaa tttgcgactg catgcgcaga acgcgggatc gccatcgcag
     1801 gggcatcact ccatgcgccg ataatggagg gtgtggaggt ggttgtcggt gaaggcgagg
     1861 gtgttttgga tggttttggg gtgggcgtat caatcacagg ggtgccttct ttcggatgag
     1921 tggttggtgc agcggtcaga ttgtttgcac aggcgctctg aacaagcaaa agcatagaga
     1981 agatcaaggc attcaatccg cgtgcaaaac gcagggggaa tcgcatggtg gcatttgggt
     2041 tgatattcat ggacagaatc tatcatcagc acaaatgcat gacaacctac tggagaaggg
     2101 gaaagtgaaa aacggtctcg gagcatcctc caagaccgtt tttgctgcag acgaaaattt
     2161 ctggttgtga atccctagtc catgcgctcc aaaacaatga cagggatcac acggctggct
     2221 ttgcggcgat aaacgtcaaa cccaggcatg acctccacca ttttgtcgta caggcgggaa
     2281 cgttcgggtt cttcagcaac cgaggctttg gcttgaaagg tttccatccc cacctcaacc
     2341 gtcaccagtg gattggcaac cacattgtga taccaatcag gatgggtagg cgccccgctc
     2401 ttggaggcga tcacaaccaa ccgctctccg tcgctgatac aagccaccgg gttaatgcgc
     2461 tctttctgac tctttgcccc aatggtgtgc aaaagaagca gggttttgcc ttcaaatggc
     2521 ccgcccactt tgccttcatt ggctcgaaac tcattaatga ttctttgatt atagtcgttc
     2581 acttcgccca ttatttgtaa ttctcctttg aaaatatatg ctcccgatgt cgccaaaaat
     2641 taagttctta ccatcgcagt aacctggaaa aacgtttagg tcagcaactg cgggttgcaa
     2701 aaaaatggcc tcggacatgg agagtcaagg attccatctg cactagccca caaaatacag
     2761 agtgcatcta tccgattcaa ggtagaataa aaaaatcagt ttcaaaaaca ggagatttga
     2821 tggaacgaaa aacacaaacg ggctatttgg tactcgccga tatttcaggg tacacatcct
     2881 ttgtcgccaa aaccgagatc gagcatgcag agaaggctat ttccatttta ttgggaaata
     2941 ttattgaaag attggacgac ctgctttcca tcgtcaaact ggaaggtgac gctgtttttg
     3001 catatgtttc agatgagcag gtgcccgact gcaatatcat gcttgaactg ctcgataaaa
     3061 cctatgagat tttccgagac tcagccaggt ctttgcacgc acacgcaacc tgtccatgca
     3121 aggcgtgtcg ggccatcccg gaacttgatc tgaaattttt ggttcaccat ggggaataca
     3181 tcgttcagca agtggcaggg atcaaagacc ttatggggaa cgatgtcacc ctgatccaca
     3241 ggcttgcaaa gaatagcgtc tctgaatcca cggggtggaa tggatatgtt ctttttacag
     3301 gcaactgtct ggaacgtatg caggcggata agaggccgtt cattcagcaa gccgaaacct
     3361 atgagcacct tggcaatgtg gaaacatacg taatgaacat gcatgtccgc tacgatgaaa
     3421 tgaaaaaata aacccgcttt atatgggaac atcaaaaacg gtctctggtg aaatattcac
     3481 gagaccgttt ttatttctgc agggcgaatc ccgtactccg tagtacaatg aaatcaggtc
     3541 acttgatggt aaattgccta tatgcggggg atttgaagta tcgcttgtaa gcagcccaac
     3601 gaaaacctca tcacatcgga acaagccaca gccgcctcat ggaaggtgta tctggcgatt
     3661 cagtccgaaa caaaaatcag gcaacaccaa aaggagcaag tcaaatgagt gaaaccacca
     3721 ccaagatcaa agccaagcgc ccatcgaagg gtatggccaa acacaaccgt ctggttaagc
     3781 aggaagcccg caagaacagt gttcccggca caacggtcaa aaagggcaaa cgccccgaag
     3841 taaaataaaa ccgcggacta aaaagtggtc acgtgacatc aagccacgtg accacttttt
     3901 tgatttaaca tttcccatcg taaacggtgt ctaaagccgt ttcactttta caacgtcctc
     3961 acatacctga attgcttata cccgggcggg tcggcattga tggcagagtc aaccgtcaca
     4021 tgaagcaagg ccggtttgcc agaatcaacc gcccgttgga gtgcggcggg caacacatcc
     4081 accgaatcaa ctttttcggc gtagcatccc aagccttgcg cgatcaagtc gtagcgcatc
     4141 tccgtggaaa tattcacgcc ctgctgcaac tccgcgggca cgccgccgaa gttgtgcgcg
     4201 ctgcgctcca tgccccagcc atcgtccaca gccacgagca caatgatctg cgcattctcg
     4261 cgcacagcag tttccatctc catgatgttg aagcccgccg ccccatcgcc cgtgatgcaa
     4321 tatacaggtc gcccgggcgc ggcaagtttc gcgccaatgg caaacggaat gcccgtgcca
     4381 agatagccca tcttcaccga atacagaaaa ctgcgcggct cataaatttg attgaacgcc
     4441 acgccccaca aggttgtatt gccgccatcg agcacggtca ccgcgttgcg cggaaagaaa
     4501 ctgcgcgcgt tgaacaccat ctgcccaggg ttgagtccgc gtgtgggctg cgccatcaaa
     4561 aattgaaaac cgttcgccat cgtctgctga ctcaactcac gataacgcgc gaggtcgggc
     4621 atttcactgc gggcggcggt cttctctttc actttggata agatcgcctt aagcgcagac
     4681 ttcgcatctg cgaggatgcc cacatctaca ggccgattca acccgatgac attcgcatcg
     4741 acatcgattt ggattgtctt ttgtttctct ggcattcccc acgctggatg agaaccccag
     4801 ccatcgtatt cacccagccg cgaaccgacc acaaggacaa catccgcttc attacgtgcc
     4861 agagttgtcg cctgcatatc aaaaaggaag aagtaatgcg ggtggtcttc agggacagcg
     4921 ccgcgcgcgc ccatgctcga gcccatgccc gcggcgagat aatttcccaa ctccaaaaat
     4981 tctggggcgg catctgccca caacacgccc ttgccagcat ggagataagg ttttttggaa
     5041 ttcgccagca actcggcagc ttcttcgatc caagcagggt cacccgcgcc catatcggtc
     5101 atgcgatatt tggatgacgg atagacaggt actttgtccg aatcaatttt tgcgccgagc
     5161 aattca
//
LOCUS       DAOQJC010000200         5099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_9586_length_5099_cov_9.267050, whole genome shotgun sequence.
ACCESSION   DAOQJC010000200 DAOQJC010000000
VERSION     DAOQJC010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9586_length_5099_cov_9.267050"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1494
                     /locus_tag="PLE39_18455"
     CDS             88..1494
                     /locus_tag="PLE39_18455"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNE70372.1"
                     /translation="MSKKFFTVLLLAVFMLAQFSVASAATVAGDASITNIPDFANLTD
                     VDVDWSVTGMAAGPKYILIWSRIQADPPVALYTGCMDIATINGSSDASGTFTYDLTLI
                     GPNNGDVVEFIVTVDSTACAGVPDIPVDTDAPMGDTTIDSFAPEYFNANPPSITGLPK
                     TIDPVACNTFEYWAMATDHYMFPSTIGYSGIGAWNLQVTGTFAPPAPVGPGEDLLSWK
                     FTFPSTASGAWTVSHQVEDQAGNQNGAPSYFRNALVITPAELEDCTTFSDVSGHEYEV
                     YIRYLATLGLVSGFDDGTFGPDTTLTRAEAATLFEISNGYDATLLPTSAPAGCEFTDV
                     AASDWFAGWVWQACSDGFMSGIGDGRFDPNNLLTRGQIVTIFNNVANVISSNPGVIGG
                     YMDTDNTLAIAYPTALVLRTAAWMDVPIGAYYAEPVQLAYGWGVADGTSDTTFSPDQP
                     ATRGEYIKMLYRALSIVN"
     gene            1714..2355
                     /locus_tag="PLE39_18460"
     CDS             1714..2355
                     /locus_tag="PLE39_18460"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HNE70373.1"
                     /translation="MFVGERMSSPVISVSPETPIHDALVMFKKEHIRRAPVIKDGKLL
                     GIVSERDLLNASPSPVTSLSVWEMNYLMSKVTVKQVMTKKVKCLDANTPIEEAARLMA
                     DSKIGGMPVMRSGMIVGMITETDLFKIFLELMGAREKAIRVSALVEDKPGQLAKVTKA
                     IADAGGNFISFGIFSGPDTSSKLLTFKVAGMKKDAVKATLEKVVKKFWDIRQS"
     gene            complement(2421..3281)
                     /locus_tag="PLE39_18465"
     CDS             complement(2421..3281)
                     /locus_tag="PLE39_18465"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNE70374.1"
                     /translation="MEERLQKLLAQAGYGSRRHCEEFIIAGRVRVNGQVATLGQKADL
                     SVDKVTLDGKALPKAESLAYTYIALYKPRNVLSAAEGQDDRETVRDLIPLEGHLYPVG
                     RLDFDSEGLILMTNDGELTNKLTHPRYGHEKEYRVLLARRPDEEQLSAWRRGVVLEDG
                     DKTAPADVNFLSTSGKGAWIRVVMGEGKKRQIREVGKLLGLPVVKIIRLRIGTLKLGT
                     LKPRQWRYLTEDEIADLKGAKREPKPAFAKPDRRSDPKKSWARNKDSKDKPPRALQRT
                     PGKPVRKSSR"
     gene            3447..4460
                     /locus_tag="PLE39_18470"
     CDS             3447..4460
                     /locus_tag="PLE39_18470"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNE70375.1"
                     /translation="MNKKNAKTLDDIARLAKVSKSTVSRALNNSPLLNQQTKERIQTL
                     ARKHNFRVNVSARNLRIRQSHTIAFVTPDCEPNFFSPESLFGFEILGGIGNELHSLGY
                     DLLIANVNPMDTTPLNSYIGSGRADGFILLSSKNSQAIIDKMIELDAPFITWGLPLPN
                     FNYCSVDGDNITGGTLATNHLIQNGRQHIAFLGGPPEELTVQHRYQGYENAFQANGQR
                     VAAKRVVYGDYSYTSGLDAMQRLLKQSPDLDAVFVNSDLMAIAAIKVILESGRRVPED
                     IAVVGYDDVSIAMYNNLPLTTIRQNVPMAGSLLAQNLIRHIKTGEITNVTTPVELVIR
                     KSA"
     gene            4529..>5099
                     /locus_tag="PLE39_18475"
     CDS             4529..>5099
                     /locus_tag="PLE39_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE70376.1"
                     /translation="MSFVTKVKQYPKIGLVILSFVAFVALGMPDGLLGVAWPSIRSGF
                     SIPLDALGMFLTALVVGYMTSSFFSGPIVTRLGVGKVLAISCTLTGVALFGYTFVPEW
                     WMMVLLGVVAGFGAGAIDAGLNTYVAAHFSEGLMQWLHASWGVGITLGPIIMTLGLST
                     TETWYTGYRIVGGFQLFLAACFVLTLPMWS"
BASE COUNT         1227 a         1345 c         1204 g         1323 t
ORIGIN      
        1 aacggaattg acatgttcct caatcgctta tacaatgctc gtattcagac caatttagtt
       61 tgaaattcat ttttcgagga gaaacgaatg agtaaaaaat ttttcactgt tttgcttttg
      121 gctgtgttca tgctggccca attcagtgtg gcctccgcag ccactgtagc tggcgatgcc
      181 tccatcacca acatccctga tttcgccaat ctcactgacg tggatgtgga ctggtcggtg
      241 actggcatgg cggccggccc caagtacatt ttgatctgga gccgcatcca ggcggatccc
      301 ccggtcgctc tctacaccgg ttgtatggat atcgccacta tcaatggctc cagcgatgcg
      361 agcggcacct tcacctatga cttgaccctc attggtccca ataacggcga tgtggtggaa
      421 ttcatcgtca ccgtggatag cactgcctgt gctggcgtgc ctgacatccc cgttgacacc
      481 gatgcaccca tgggcgacac cactatcgac tcttttgcgc cggagtattt caacgccaac
      541 ccgcccagca tcacaggtct gccaaagacc attgaccctg tcgcctgcaa taccttcgaa
      601 tattgggcca tggctactga ccactacatg ttcccatcca cgattggata ttctgggatc
      661 ggagcttgga atcttcaggt taccggcacc tttgccccgc ctgctcctgt tggccctggc
      721 gaagacctgc tctcttggaa attcacattc ccttcaactg ccagcggtgc atggacagtg
      781 tctcatcaag tggaagacca ggctggaaat caaaacggtg ctccgtccta ttttcgcaat
      841 gcccttgtca tcacacccgc agaattggaa gattgcacaa ccttctctga tgtaagcggc
      901 cacgaatatg aagtgtacat ccgctacctc gccaccctgg gcctggtcag tggtttcgat
      961 gatggcacct tcggccccga caccaccctc acccgcgccg aagccgccac ccttttcgaa
     1021 atttccaacg gctacgatgc caccctgctt cccacctctg cccctgctgg ctgtgaattc
     1081 acagatgtag ccgcaagcga ctggttcgct ggctgggtct ggcaagcctg ttctgatgga
     1141 ttcatgagcg gcattggcga tggcagattt gacccgaaca acctgctcac ccgtggtcag
     1201 atcgtcacca ttttcaataa tgttgcgaat gtaatttcct cgaatcccgg cgtcatcggt
     1261 ggatacatgg ataccgataa cacccttgcc attgcttacc caacagccct tgttttgcgc
     1321 accgctgcat ggatggatgt tcccattggc gcctactatg ccgaacctgt tcaactggcg
     1381 tatggctggg gcgttgctga tggcacctcc gacaccacct tctcacccga ccaacccgcc
     1441 acccgcggcg aatacatcaa gatgttgtac cgcgctctga gcatcgtgaa ctaagtttct
     1501 gaatcaagtt aggttgacgg gcgaatccaa tttgggttcg cccgttttgt tttaacgcag
     1561 aaatcacccc aacaaattga gatatgacat ccatgcgcca ccgcaaagtg accttattca
     1621 ctccaattta gtgactttct tttcccgaaa tcctcttata atgtgtttgt aacaaatttc
     1681 acaacacgtc cttttactac cctggaggta aaaatgtttg tcggcgaaag aatgtcgtcc
     1741 cctgttattt cagtctcccc cgaaaccccg atccatgatg cgctggtgat gttcaagaag
     1801 gaacacatcc gccgtgctcc ggtcatcaaa gatggaaaat tacttggtat tgtttctgag
     1861 cgcgatttgc tcaacgcatc tccctcccct gtcaccagcc tgagcgtatg ggaaatgaac
     1921 tacctgatga gcaaagtcac tgtcaaacag gtaatgacca agaaagttaa gtgcctggat
     1981 gcgaacaccc ccattgaaga agccgcccgc ttgatggctg attcaaaaat cggcggcatg
     2041 cccgtcatgc ggtctggcat gatcgtgggt atgatcaccg aaaccgacct ctttaagatc
     2101 ttccttgagc tgatgggcgc ccgcgaaaag gccatccgcg tcagtgcctt ggtggaagat
     2161 aaacccggtc aactggcaaa ggtgaccaaa gccattgccg atgcaggcgg aaacttcatt
     2221 tccttcggta ttttctctgg cccagacaca agctcgaagc tgttgacctt caaagtggct
     2281 ggcatgaaaa aagatgctgt caaagccacg cttgaaaaag tggtcaagaa gttctgggat
     2341 atccgccaaa gctaattttc tgaaaccagg ttaaaagtaa aaccacccgt gaatactgat
     2401 tgcgggtggt tttcgttttt ttatctggaa gattttcgaa cgggcttccc gggtgtccgc
     2461 tgaagggcgc ggggtggttt atccttcgag tctttatttc ttgcccagct tttcttcggg
     2521 tctgaccgcc tgtcaggttt tgcaaaagca ggtttcggct cgcgttttgc gcctttcagg
     2581 tcggcaattt cgtcttcggt cagatatctc cattggcggg gtttaagtgt gccaagtttc
     2641 aaggttccaa tccgcaaacg gatgatcttc accacgggca ggcccagcag tttccccacc
     2701 tcacggatct ggcgcttttt cccttccccc atgaccactc gaatccacgc acccttccca
     2761 gaggtggata agaaatttac atcggcaggg gcggtcttat cgccatcttc caagacaact
     2821 ccgcgacgcc atgctgaaag ttgctcttca tcaggtctac gagcaagcaa aacacggtac
     2881 tctttttcat gcccatagcg ggggtgtgta agtttgttgg tcagttcacc gtcattggtc
     2941 atcaagatca gaccttctga atcaaaatcc aggcgaccga cgggatataa atggccttcc
     3001 aggggaatca aatcgcgaac tgtttcgcgg tcatcctgac cttctgctgc agaaagtaca
     3061 tttcggggtt tgtaaagcgc aatgtaggta tacgccaatg attcggcttt gggtaaagcc
     3121 ttgccatcca gagtaacctt gtcgacagat aaatcggcct tttggccaag agtggccacc
     3181 tgcccattga cccgcacacg tcctgcaata ataaattctt cacaatggcg gcgagatcca
     3241 tacccagcct gagcaagtag tttttgtaat cgttcttcca tagagacagc attataacgg
     3301 atttcagtga cgggcacggc ggttcgaatg aatctaaatc gaacaccgat acacacctgt
     3361 acaaataggc ctctgacctt caaattcttg agccgataca acagcgaaca gtgctataat
     3421 gcaatcgatt gcaaagaaag tcacccgtga acaaaaaaaa cgcaaaaacc cttgacgaca
     3481 ttgcccggtt ggcgaaagtt tccaagtcaa cagtttctcg tgctttaaac aacagtcccc
     3541 tccttaacca acaaacaaaa gaacgcatcc agacacttgc caggaaacat aatttccgtg
     3601 tcaatgtttc tgcacgcaac cttcgaatac gccaaagtca caccattgcg tttgtaacac
     3661 ccgattgcga gccgaacttc ttttcccctg aaagtctgtt cggcttcgag atattgggtg
     3721 gaatcggaaa tgaactgcac tcactaggat acgacctctt gattgcaaac gttaatccaa
     3781 tggatacaac tcccctcaac tcctatattg gcagtgggcg ggcagatggg tttattctat
     3841 tgtcatcaaa aaacagccaa gccatcatcg acaaaatgat cgaactggat gcacccttca
     3901 tcacatgggg attgccactt ccaaatttca attattgttc cgttgatggg gataacatta
     3961 ctggtggaac gcttgcaaca aaccacctta tccagaacgg caggcaacat attgcatttc
     4021 tgggcggccc tcccgaagag ctgaccgtgc aacaccgtta tcaaggatac gaaaatgcat
     4081 ttcaagcaaa tggacaacga gtggccgcaa aacgggtggt ctacggagat tattcttaca
     4141 cttcgggttt ggatgcgatg caacgccttt tgaaacaatc ccctgacctt gatgctgttt
     4201 ttgtaaacag tgatctgatg gcaattgccg cgatcaaggt cattctcgaa agtggacgac
     4261 gcgttcctga agatattgca gtggttggtt atgacgatgt atccattgcc atgtacaaca
     4321 atctgccatt gacaacaatt cgacaaaacg ttcccatggc aggtagtttg ttggcgcaga
     4381 atttaatacg gcatatcaaa acaggtgaga tcaccaatgt aaccacacct gtggaattgg
     4441 tgatccgtaa atcggcgtag ggtattgctt tttttgtaaa tagctgcaat cgattgcaga
     4501 aaacaaaacg taaaggaaga atatcttcat gagctttgta acaaaagtaa agcaatatcc
     4561 caagatcggt ttggtcattt tgtctttcgt tgcctttgtt gctttgggca tgcccgatgg
     4621 cttactaggt gtagcatggc cttccattcg cagcggattt tccattccgc tggatgcact
     4681 cggcatgttc ttaaccgctc tggtggtcgg ctacatgacc tccagtttct ttagtggacc
     4741 catcgtgacg cgcctgggcg taggcaaggt tctggcgatc agttgtaccc tgaccggtgt
     4801 tgcgctgttc ggctacacct ttgttccaga atggtggatg atggtgcttt tgggtgtggt
     4861 cgccgggttt ggtgcaggtg cgatcgatgc aggtttgaat acgtacgtcg cggcgcattt
     4921 cagcgaaggg ctgatgcaat ggctccatgc cagttggggc gtgggcatca cgctgggacc
     4981 gatcatcatg accctcggat taagtactac agagacttgg tacacgggct atcgaatcgt
     5041 tggcggcttt caactctttt tggcggcttg ctttgtgttg actctgccca tgtggagcg
//
LOCUS       DAOQJC010000201         5069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_9677_length_5069_cov_7.947946, whole genome shotgun sequence.
ACCESSION   DAOQJC010000201 DAOQJC010000000
VERSION     DAOQJC010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9677_length_5069_cov_7.947946"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PLE39_18480"
     CDS             complement(<1..658)
                     /locus_tag="PLE39_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70377.1"
                     /translation="MDVDSGGIYTIGGFFDTADFDPGSNVFNLTSLGNNDIFVSKLNK
                     DGSFAWAISIGSTGYLEYAHDVTLDASGNVYVIGTFGGTIDFNPGAEVFNMTSKGVHD
                     VFILKLDENGNFVWAKSMSGADGNSKGEGTSIILDFSDNIYLTGSFSGVVDFDPGSGV
                     HNLTSTPYGDNAFIVKLNGNGGFMWVKSMGVTVDGYTFPSVNSRDIVVDKNGNVYTAG
                     G"
     gene            complement(649..840)
                     /locus_tag="PLE39_18485"
     CDS             complement(649..840)
                     /locus_tag="PLE39_18485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70378.1"
                     /translation="MKTSSKFVFSMLALLMLLAMLFGAAPVQSVQAEAAESPLAASGD
                     FVWAKGMGGRVMSMFLIWM"
     gene            complement(989..3130)
                     /locus_tag="PLE39_18490"
     CDS             complement(989..3130)
                     /locus_tag="PLE39_18490"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNE70379.1"
                     /translation="MNNITAKHILYSLLLAGVALLGVYFLPTGNTFQITVETPTSIAS
                     SPMITGVSGDFLWAKRMGGSGTGDFGQGYDIATDLDGNLYFTGGFTGELDFDPGEGTF
                     TLASASLDYSDIFAAKLDSEGNFLWARHIGGQAWDYAYDIAVDSNRDVYVMGSFEGTA
                     DFDPGPDILNLSSAGLRDIFVVRLDENGNLIWAKRMGGASDDGGSAVAVDHDGNVYLT
                     GYFQGTADFDPGENTANLKSAGKNDIVVTKLDADGNLIWAKRMGGIQNDLGFGLALGA
                     DSVVYTTGRFEGAVDFDPGAETTNFVSIGGSDAFLSKLDADGNFVWAGVVGGNGEAQG
                     FGLVVDSGGSVYLTGDFWRKVDFDPGEKSFDLTSFGDSDVFLCKLDENGNFVWAKQMG
                     GPGAGHGYDLALDPSGNIYTVGSFSDTADFDPGAGEFHLAGNGRYEDIFIAQLDADGN
                     FIWAKSAGGAGDDLGVGIAVDPRGDVVATGNFAGKVDFDPGPEPFYLASQGYYDVFVS
                     KLNGNGIPIFADVGDDHPQFKYIQALYASGLTSGCEVNPLKFCPDAVLNRAQTAVFML
                     RGQFGPAYTPPPAPWDVFSTEDWSGFEWARPWAEGMYAAELTKGCQADPLKFCPTAQL
                     TRMEASIFGLKMKYGQSYLPPPATGDLFADLPAIGPNEWGIAWAEQAYRSGLLPSCGI
                     QNGKPMFCPDEPVHRALAAYIIVKARNLPIP"
     gene            complement(3237..4160)
                     /locus_tag="PLE39_18495"
     CDS             complement(3237..4160)
                     /locus_tag="PLE39_18495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE70380.1"
                     /translation="MSRYKFILPFFPLLLGILSLIFLPSLIKPEVVLSFRLGLGAVLF
                     GFGLLITLWMQSLGVVDSLREKKMRTEMEALTRSYEDSHRRFIRRLDHELKNPLMGLQ
                     ASLENLQAVTELEERRKAEGNIQKALERLVRLLRDLRKLSDLDESKLERESVDIPELV
                     EEIIEVLRSTPGREHRRINLMVTRVPTPPPPVSGDRDLLGISLYNLLDNALKFTVQNE
                     AVEVRVRDDERVVFVEVADGGSGIHPDEQQKVFEELYRGENAHAVEGSGLGLSFVKRI
                     VALHHGELTLRSQRDEQHGSIFTIRLPVTKL"
     gene            complement(4157..4876)
                     /locus_tag="PLE39_18500"
     CDS             complement(4157..4876)
                     /locus_tag="PLE39_18500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE70381.1"
                     /translation="MNQRQLTLLLVDDDAMIVESLGPLLERSGFHVITASTGEEALTK
                     VQAHHPDVIVMDVLMPRMNGREALRRMRKSNIWIPTILLTQVGEASERAVALEEGADD
                     YLNKPFHSQELLARIHAVLRRARPAGQSLSSAHRLISLDLLLDRQSRRAFLADESLNL
                     TPKAFAVLEYLMTHPDEAVSREHLLHSVWGWEYPTGTRTVDTRVNELRRALKDEAGAP
                     RFIQTLTGEGYIFIAPVKPES"
BASE COUNT         1305 a         1437 c         1203 g         1124 t
ORIGIN      
        1 agccacccgc cgtatatacg ttgccatttt tgtcaacaac aatatccctg ctgtttacag
       61 acggaaaagt gtagccatca actgttaccc ccatgctttt cacccacatg aaaccgccgt
      121 tgccattcag ttttacaata aaagcattgt cgccgtatgg ggtactggtt aaattatgta
      181 ctcccgaccc ggggtcaaaa tccacaaccc cgctaaaact cccggttaag taaatgttat
      241 cgctgaaatc caagatgatg cttgtgcctt ctcccttgga attaccgtct gccccgctca
      301 tactcttagc ccacacaaaa tttccatttt catccagttt caaaataaaa acatcgtgga
      361 cgcctttgct ggtcatgttg aacacttccg cgccggggtt gaagtctatt gtgccgccaa
      421 acgtacctat aacataaaca ttgccagagg catccaaagt aacgtcatgg gcatattcca
      481 gataccctgt gctgccaatg cttattgccc aagcaaaact gccatcttta ttcaatttgg
      541 aaacaaatat gtcattattg cccaggctag tcaggttaaa cacattggac ccaggatcaa
      601 aatcagcggt atcgaaaaaa cctccaatgg tatagatacc ccctgagtct acatccatat
      661 cagaaacata ctcataactc gcccacccat tcccttcgcc cagacaaaat ctccacttgc
      721 cgccagcgga ctttccgccg cctccgcctg cacgctctgc acaggcgccg ccccaaacaa
      781 catggccagc agcatcagca acgccaacat actaaaaacg aactttgaac ttgttttcat
      841 actacattct ccttattgat ttgaaaattt ctctccaaaa ttctgacacc ttagttatac
      901 cgaaaggttg ttacaggcat gacacagacc tgtcacagtt tggtaacatg caaaaaaata
      961 gcgcgagatt ttatctcgcg ctacaggttt atggtatagg cagatttctg gccttgacga
     1021 tgatatacgc cgccaaggca cggtggacag gctcatccgg gcaaaacatg ggcttgccat
     1081 tttggattcc gcaggaaggc aacagaccgc ttcgataagc ttgctcagcc caggcaatgc
     1141 cccactcatt cgggccgatg gcaggcaggt cggcaaacag atcaccagtg gcaggcggag
     1201 ggaggtagct ctgcccatat ttcattttca acccaaatat cgaagcctcc atgcgggtga
     1261 gttgtgccgt gggacaaaat ttcaacgggt cagcctgaca ccccttggtc aattcggcgg
     1321 catacattcc ttcggcccac ggacgtgccc attcgaaccc gctccaatct tctgttgaaa
     1381 agacatccca cggggcagga ggcggagtgt aggcggggcc aaactgtcca cgcagcatga
     1441 aaacggccgt ttgggcgcgg tttaacacgg catcggggca gaactttaat gggttgacct
     1501 cgcaacccga ggtgagtccg gaagcgtata aggcttgaat gtatttgaac tgtggatggt
     1561 cgtcacccac atctgcaaag atcggtattc cattcccatt aagttttgaa acaaaaacat
     1621 catagtagcc ctgactggca aggtaaaacg gctccgggcc ggggtcgaaa tcaaccttgc
     1681 ctgcaaagtt accggtggca accacatcgc cgcgcgggtc tacagcgatg cccacaccaa
     1741 ggtcatcccc cgcgccgcct gcgctcttgg cccagataaa atttccatcg gcatccagtt
     1801 gggcgatgaa aatatcttcg tagcggccat tgcccgcaag atgaaactca cccgcgccgg
     1861 ggtcaaaatc ggcggtatct gagaaactgc ccacggtgta gatattgccc gaggggtcaa
     1921 gcgcgaggtc gtatccatgc ccagcccccg ggccgcccat ttgtttggcc catacgaaat
     1981 ttccgttttc atccaattta cacaggaaga catccgaatc gccgaaactg gtcaggtcga
     2041 atgatttttc gccgggatca aaatcgacct tgcgccaaaa atcgccggtc aaatacacac
     2101 tgccgcctga atccacaacc aggccaaagc cttgcgcctc accatttccg ccaaccacac
     2161 ccgcccaaac aaaatttcca tcggcatcca gctttgaaag gaaggcatcg ctccctccta
     2221 tgctgacaaa gttagtcgtt tcagcgccgg ggtcaaaatc aacagcgcct tcgaaacggc
     2281 cagtggtgta cacgacggaa tcggcaccaa gagcaaggcc aaagccaagg tcgttttgaa
     2341 ttccacccat gcgttttgcc cagatcaagt ttccatcggc atccagtttt gtaacaacaa
     2401 tatcgttctt accggcgctt ttcaggtttg cagtattttc cccggggtca aaatcggcgg
     2461 taccttgaaa ataacccgtt agatatacat tcccatcgtg atcaacagcc acagcagaac
     2521 cgccgtcatc acttgcgccg cccatgcgtt tggcccagat caaatttcca ttttcatcga
     2581 gacggaccac gaaaatatca cgcaggcctg cgctgctcaa gttcaaaata tccgggcccg
     2641 ggtcgaaatc ggcggtgcct tcaaagctgc ccatcacata tacatccctg tttgaatcca
     2701 cggcgatatc gtaggcataa tcccaagcct gcccgccgat atgtcttgcc cagagaaaat
     2761 tcccttcaga atccaacttg gcggcgaaga tatcgctgta atcaaggctg gcactggcca
     2821 gggtaaaggt gccctcaccg gggtcaaaat ccagttcgcc ggtaaaccca ccggtgaaat
     2881 agaggtttcc atccaaatcc gtcgcaatat cgtacccctg cccgaaatcg ccggtaccgc
     2941 ttccgcccat gcgttttgcc cagagaaaat cgccgctgac tccggtaatc atcgggctgg
     3001 aggcgatcga cgtgggggtt tccacggtaa tttgaaatgt gttgccagtg ggaagaaaat
     3061 aaacgcccag caatgccacc ccggcaagca gaagcgaata caggatatgt tttgccgtta
     3121 tgttgttcat agttgcatca ataaagaccg ttgacagtgg accacaggcc actgtcggta
     3181 agttactgaa tgatccagtt atatcggatg aatgttacag gcatgacaca gcgatgtcac
     3241 agtttggtaa cagggaggcg aatggtaaaa atggaaccgt gctgctcatc gcgctggctt
     3301 cgcaaggtca actccccgtg atgcagtgca acgatgcgtt tgacgaaaga caaccccaac
     3361 ccgctccctt ccacggcatg ggcattttcg ccacgataca gttcctcaaa aaccttttgc
     3421 tgttcatctg gatgaatccc cgaaccacca tctgccactt caacaaagac cacccgttcg
     3481 tcatcacgca cccgaacttc aaccgcttca ttctggaccg tgaacttaag cgcgttatca
     3541 aggagattgt acaagctgat gccaagcagg tcgcggtcac cgctcaccgg cggaggcggt
     3601 gtgggcactc tagtcaccat caaattaatg cggcgatgtt cgcgccccgg cgtggaccgc
     3661 aacacctcga tgatctcctc caccaattcg gggatatcca ccgattcgcg ttccaattta
     3721 ctttcatcca ggtcagagag tttccgcagg tcgcgcaaca gacggacaag ccgctcaagg
     3781 gctttttgaa tattgccctc cgccttgcgg cgttcttcca attcggtcac ggcttgcaga
     3841 ttttccagcg aagcctgcaa acccatcagc ggatttttca actcgtggtc gaggcggcga
     3901 atgaagcggc ggtgactgtc ttcataactg cgggtcagcg cttccatttc ggtgcgcatc
     3961 ttcttttcac gcagcgaatc gaccacaccc aaagactgca tccacaacgt gatgagcagg
     4021 ccaaacccaa acagaaccgc acccaacccc agccgaaacg agagaacaac ctcgggtttg
     4081 atcagggagg gtaaaaagat taaggaaagg attccaagca ggagagggaa aaagggaagt
     4141 atgaatttat aacggctcat gactcaggct tcacaggtgc tatgaaaatg tacccctcac
     4201 ccgtcagggt ctggatgaaa cgaggcgcgc cagcttcatc tttcaaagcc cggcgcaatt
     4261 cgttgacgcg ggtatccacg gtgcgtgtac cggttgggta ttcccagccc cagaccgagt
     4321 gcagcaaatg ctcacggctg accgcttcat cggggtgggt catcaaatat tcgagaacag
     4381 caaacgcttt cggtgtgagg ttgagggatt cgtcggcgag aaaggcgcgt cgagattggc
     4441 gatccagcaa caggtcaagt gaaatgaggc gatgcgcaga ggaaagtgac tgcccggcag
     4501 ggcgggcacg tcgcagtacg gcatggatcc gcgcaagcaa ttcctgcgaa tgaaatggct
     4561 tgttgaggta atcatctgcg ccttcttcca aagccaccgc gcgttcagaa gcctctccca
     4621 cttgcgtaag caaaatggtc ggaatccaaa tattggattt gcgcatccgc cgtagggctt
     4681 cgcgcccgtt catgcgcggc atgagcacat ccatcacgat cacatcggga tggtgagcct
     4741 gcaccttggt aagcgcttct tcacctgttg aggcggttat cacatgaaac cccgaacgtt
     4801 caagcaatgg ccccaaactt tcaacgatca tggcatcatc atccacaagc agcaaggtaa
     4861 gttgacgttg gttcatggct ggaaattata cttcgctccc tatatgcaaa gacaggtgac
     4921 agtcacttgt aaagtgactg tcacctcatg gcttacggca acggcaggtt cttggctttg
     4981 acgatcaaat acgcgcccca accgcgatcc accaattgac ttgggcagaa catcggcttg
     5041 cctgtcgcag aatctgtgcc gcaggcggg
//
LOCUS       DAOQJC010000202         5049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_9739_length_5049_cov_9.010412, whole genome shotgun sequence.
ACCESSION   DAOQJC010000202 DAOQJC010000000
VERSION     DAOQJC010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9739_length_5049_cov_9.010412"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1143)
                     /locus_tag="PLE39_18505"
     CDS             complement(142..1143)
                     /locus_tag="PLE39_18505"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE70382.1"
                     /translation="MTQIITLQTALEHLLNSNADIPKNHLQHYSDLDPQSLTIFMDVW
                     PRVKPTRKLLLLDELLSHLDEDTIVSYEEIGRALLNDANSDVRSRAIRLLAESDDPKL
                     ASKFIEILLNDPDLAPRMESATLLGEFILMGELEKLKQSLQQKIEGALISVIRSEDNP
                     SLRRRALEALSYSSNPEVAALIESAFERADPAWVAAALRSMGRSHDERWNEDVVSRML
                     DDDPRIRFAAVEAAGELNIEEAGPILIKMLEDEEEDDDVVAAAIWSLSQIGGDDARIY
                     LLNLIENTEEEELVEFLEDALENLDFNTELNKFDLLSLDDDDDLLELDEEELEDEDD"
     gene            complement(1147..2259)
                     /locus_tag="PLE39_18510"
     CDS             complement(1147..2259)
                     /locus_tag="PLE39_18510"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNE70383.1"
                     /translation="MVSGFVSLIVFLLALGGMIFVHELGHFIAARWAGVEVEEFGFGL
                     PSYRIAKLFTWQGTDFTIHALPLGGFIRPKGENDPNVPGGLASANPWKRLVVLAAGPF
                     MNLLTAVVVFSFLIASEGVPVTGSIKIDAVSDNSPAQAAGMLPGDILLAINGEKVTEV
                     DAAIAIIQANLDKPVELLMDRNGEEVTLTATPLSSRLKSEGALGIGLGYPTRPATVIE
                     SISGGFMITGAQAAMIVYLPIALIQGAIAPEEARFIGFKGIFDMFDVAVEEDVTTRQE
                     VAAATAGTPTPQPTNWTLNLIGILSISLGVMNLFPIPALDGGRILFTMPEILFRKRIP
                     SEWENMVNGIAMLLLICLMLFVNVMDFVNPAQIPTP"
     gene            complement(2398..3369)
                     /gene="mvaD"
                     /locus_tag="PLE39_18515"
     CDS             complement(2398..3369)
                     /gene="mvaD"
                     /locus_tag="PLE39_18515"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HNE70384.1"
                     /translation="MKPSSATAQANPNIAFIKYWGNRDNTYRLPSNSSISMNLDGLFT
                     RTTVSFTSAKADSLAINAAEVTGKGLDRVSYILDLVRKIANINERAEVTSENNFPAGA
                     GIASSAAAFAALALAASKAAGLNLSEPELSVLARRGSGSASRSIPGGFVEWQMGREEA
                     DSYSFSIAPADHWNLVDCVAIVNATHKKTGSTEGHAIAGTSPLQNARVADAPRRLEIC
                     RNAILQRDFEAFANIIEHDSDMMHSVMMTSNPPLMYWQAATVEIFHQVREWRASGLPV
                     GYTVDAGANVHVLCLGDSAIEVEKRLREIPGVSNVLVAGVGGAAKIV"
     gene            complement(3512..4690)
                     /locus_tag="PLE39_18520"
     CDS             complement(3512..4690)
                     /locus_tag="PLE39_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70385.1"
                     /translation="MPIPYLLTTLLYTFVAMFVAADASLVSMTVFSAFPALRWVRVHF
                     ITLGILSQLVFGVLPILTASLSKKEKPAMRWDIWLMLNAGLVTLVAGFASMNHPMIFT
                     GGTLIFIAALLLILQLWNIRGGDAPASLKFYITGIFYLLVGIIIGTGLFLNWSDALYI
                     KVPLEAHIHANSWGFMSLVFAGLMVDVIPMVTGKPLASKNATAYMFWGMTLGAFGLVL
                     GPWLGGSLPPTVVGLILHISATVVLVISLVRSLKQNGKLTTAGGWHLVSSYIWILLPV
                     LMAPTILLGIFEAGPIESTAPQALIYGWVLQFGIAVIPYIARRFFLKEENPQLGGCWG
                     SLTAANLGSVLVWVSIFSGPAKGIVYGIAFVFYVLALIHPLKELAEITRNGLKKYELA
                     "
     gene            complement(4709..>5049)
                     /locus_tag="PLE39_18525"
     CDS             complement(4709..>5049)
                     /locus_tag="PLE39_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70386.1"
                     /translation="GVLAFVIGALSIFAGGMAMRGWNPGYSVLGWLPVYNFVMGVLTV
                     FVPAILLWKENRHAKTAALFFFGIHAGATVLLLLAFRDVVAIQSLLAMTFRLIVWLVI
                     LALIHASVKK"
BASE COUNT         1291 a         1304 c         1320 g         1134 t
ORIGIN      
        1 ttggttaaga gttcgaccct cttcgcgctc acaaatgccc tcctcattgg agggcttttt
       61 gtttggttca gagcccccgt aggcataaaa aaacgctgac aggggatttg tcagcgtttt
      121 tgcttctaaa tttagggcag attaatcgtc ttcgtcttcc agttcttctt catccagctc
      181 caaaagatcg tcgtcgtcat cgagggatag caggtcgaac ttgtttagtt cggtgttgaa
      241 atcgaggttt tcaagggcgt cctcgaggaa ttcgaccagt tcttcttctt cggtgttctc
      301 aatgaggtta agcaggtaga tgcgtgcgtc atcgccgccg atttgtgaaa gcgaccagat
      361 cgccgcggcg accacatcat cgtcctcttc ttcatcttca agcattttga tgaggatggg
      421 gccagcttct tcaatattga gttctccagc ggcttccaca gcggcaaagc ggatgcgtgg
      481 atcatcatcc agcatgcggc tgacgacatc ttcattccag cgttcgtcgt gagagcggcc
      541 catggaacgc aaagcggcgg caacccacgc tgggtctgcg cgctcaaaag cagattcgat
      601 cagggctgcc acttccgggt ttgaggagta gctgagggct tcaagggcgc gcctgcgcag
      661 cgatggatta tcctcactgc ggatgacgga aatcagcgct ccttcgattt tttgttggag
      721 gctttgcttc agtttttcca actcgcccat caaaatgaat tcacccaaca gggtggcaga
      781 ttccatccgt ggggcgaggt ctggatcgtt gagtaagatc tcgataaatt tgctggcaag
      841 tttcgggtcg tctgattcgg ccagcaggcg aatggcgcgt gagcgtacgt cgctgttggc
      901 atcgttcagc agggcacgac cgatctcttc gtaggaaacg atggtgtctt cgtcaaggtg
      961 agataaaagc tcatcgagca ggagcagttt tcgggtcggt ttgacgcgcg gccagacatc
     1021 cataaaaata gtcagcgatt gcgggtcaag gtcagaataa tgctgaaggt gattcttcgg
     1081 aatatctgca ttggaattca gcaaatgttc gagcgcggtt tgaagggtga taatttgtgt
     1141 catagattat ggggtgggga tttgagcggg gttgacgaag tccatcacat tgacgaagag
     1201 catcaggcag atcagcagaa gcatggcaat gccattgacc atattttccc attcgctggg
     1261 gatgcgtttt cggaaaagga tttcaggcat ggtgaagaga atgcggccgc catccagtgc
     1321 ggggatgggg aagaggttca ttacaccaag tgaaatgctc agaatgccga tcaggttcag
     1381 ggtccagttt gtgggttggg gagtgggtgt gcctgctgtg gcagcggcaa cttcctgtcg
     1441 tgtggtcacg tcttcttcca cagccacatc gaacatatca aagatccctt tgaacccgat
     1501 aaagcgtgcc tcttcaggcg caatggcacc ctgaataaga gcaatgggta gatatacgat
     1561 catggcggcc tgtgcgcccg tgatcatgaa accgcctgaa atactttcga tcactgttgc
     1621 aggccgtgtg ggatacccca acccgatgcc gagcgcgcct tcacttttca atctgcttga
     1681 aagcggtgtg gcagtgagcg ttacctcttc accgttgcgg tccatcaaca actcaacagg
     1741 tttatcgagg ttggcttgaa tgatcgcgat ggcagcatcc acttcggtga ccttctcgcc
     1801 gttgatggca agcaggatat ctcccggcag catccccgcc gcctgggcag gggagttgtc
     1861 agagacggca tcgatcttga tcgagccagt tactggcaca ccctctgaag cgatcaaaaa
     1921 cgagaacacc acgacagcgg tcaaaagatt catgaacggg ccagcggcca gaaccaccag
     1981 ccgtttccac gggtttgccg aagccaaccc gcccggtacg tttggatcat tttcaccctt
     2041 cgggcgaata aaaccaccca gcggcagcgc atggatcgta aaatcggtcc cttgccaggt
     2101 gaacagtttg gcgattctgt aggaaggcag accaaaacca aattcttcca cttcaacacc
     2161 cgcccaacgt gccgcaatga aatgccccaa ttcgtgtaca aagatcatcc cgcccaaagc
     2221 gagcaggaaa acaattaaag atacaaaacc agataccatt ttttatcctt tgcctagttt
     2281 aaacgacaaa ttggattata tcttcaaatg gatttgccga agttaatctt ttattagacg
     2341 aaaagcgcgg tcatactcaa ccgaaacctg accctgtttc gaaggacagt ttctccccta
     2401 aacaattttc gccgcgccac cgaccccagc caccagcaca ttgctcacac cgggaatctc
     2461 acgcagtcgt ttttccactt cgatcgcgga atcacccaaa cacagcacat gcacattggc
     2521 gcccgcatcc accgtgtaac ccacgggcaa tccgctggca cgccattcac gcacctgatg
     2581 aaagatctcc accgtggcag cctgccaata catcaacggc gggttggagg tcatcatcac
     2641 tgaatgcatc atatctgaat catgctcgat gatattggca aaggcttcaa aatccctttg
     2701 caaaatcgca tttcggcaaa tttcgagacg ccgtggggca tctgccacac gcgcattttg
     2761 cagcggactt gtcccagcaa tggcatggcc ctccgtggag cccgtctttt tatgcgtcgc
     2821 attgacaatg gccacacaat cgaccagatt ccaatggtcg gctggcgcga tggaaaaaga
     2881 gtacgaatcg gcttcttccc ttcccatttg ccattctaca aaaccacccg gtatggagcg
     2941 tgaagcggac cccgatccgc gccgggccag caccgagagt tcaggctcgc tcaaattcag
     3001 ccccgccgct ttgctggcag ccagggctaa tgccgcaaac gccgccgcag aagaggcaat
     3061 tcccgccccg gcagggaagt tgttctcgct ggtcacttct gctctttcat ttatgtttgc
     3121 gatcttccgt accagatcga gaatgtagga tacacggtcc aaccccttgc ctgtcacctc
     3181 cgcggcatta atggccagcg aatctgcttt tgcagaagtg aacgaaacgg ttgtgcgggt
     3241 gaataatcca tccaaattca tcgagatgga agaattggac ggcaggcgat acgtattgtc
     3301 gcgattgccc caatatttga tgaaggcgat attaggattt gcttgggcgg tggcagagga
     3361 aggtttcatg ggaaagtctc gtggaaacag gtgatttaat taactcaaat tatgacaggt
     3421 tttaattttt tagagaagca aatcaagttt accatgtgta aatccatcaa aaaagcacag
     3481 gtcaattgcc tgtgcttttt tgatcttgtt cttacgctaa ttcgtatttc ttcaatccat
     3541 tgcgagtgat ctcagccagt tctttaagcg gatggatcag cgccagcaca taaaaaacga
     3601 aggcaatgcc ataaacgatc cctttggcgg ggccgctgaa aatgctgacc caaacgagga
     3661 cactgccaag gttggcagcg gtcaggctgc cccagcaacc gcccagttga ggattttctt
     3721 ctttgaggaa gaaacggcgg gcgatatatg ggatgacggc aatgccgaat tgcagcaccc
     3781 agccatagat caaggcttga ggcgcagtcg attcgattgg gcctgcttca aaaataccga
     3841 gcagaatcgt gggtgccatc aacacgggca gcaagatcca aatgtaggaa gagacaagat
     3901 gccagccgcc tgctgtggtc agtttgccgt tttgcttgag cgaacgcacc agcgagatga
     3961 ccaacaccac ggttgcagaa atgtgcagga tcagcccgac aacggtgggg ggcaagcttc
     4021 cacccagcca cggacccaac acgaggccga aagcgccgag ggtcatgccc cagaacatgt
     4081 aggcggtggc atttttagaa gcgagcggtt tccccgtgac catcggtatg acgtccacca
     4141 ttagaccggc aaagaccagc gacatgaagc cccacgagtt ggcgtggatg tgtgcctcaa
     4201 gcggaacttt gatgtagagc gcgtcactcc agttgaggaa caaacctgtg ccgatgatga
     4261 tgccgaccag caaatagaaa atgcccgtga tgtaaaactt gaggctggca ggcgcgtcac
     4321 cgccgcgaat gttccagagt tgcaaaatta gaagcaatgc tgcgatgaag atcagtgtac
     4381 cgcctgtgaa gatcatcggg tggttcatgc tggcgaatcc tgccaccaga gtcaccaaac
     4441 ctgcattgag catcaaccaa atatcccaac gcatggcagg cttctctttt ttggaaagcg
     4501 aagcagtaag aatgggcagt acgccaaaga ccagttgcga aaggatgccc aatgtgatga
     4561 agtgaacacg gacccagcgc aaggcaggga aggcgctaaa aacggtcatg ctgacgagcg
     4621 aagcatctgc tgccacgaac atggcaacga acgtgtaaag caaagtagtg agtaaatatg
     4681 ggattggcat tttgttttcc ttgagaacct attttttgac cgaagcatgg atcagcgcga
     4741 gaatgaccag ccagacgatc aggcgaaagg tcattgccaa aagactttgt attgccacca
     4801 catcacgaaa ggccagcaaa agcaaaacgg tcgcccccgc atgaattccg aagaagaaca
     4861 gcgcggcagt tttcgcatgg cggttttcct tccaaagcaa aatcgctgga acaaaaacag
     4921 tcaacacgcc catcacaaag ttataaacag gcagccagcc cagtacagaa tagcccggat
     4981 tccatccgcg cattgccatg ccgccagcaa agatcgagag ggcaccgatc acaaacgcaa
     5041 gtacgcccg
//
LOCUS       DAOQJC010000203         4972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_9969_length_4972_cov_9.671751, whole genome shotgun sequence.
ACCESSION   DAOQJC010000203 DAOQJC010000000
VERSION     DAOQJC010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9969_length_4972_cov_9.671751"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLE39_18530"
     CDS             <1..474
                     /locus_tag="PLE39_18530"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNE70387.1"
                     /translation="METPPVSIALEQLGVPHTIFRHDDARPVHSLEQAASDRGQRASQ
                     VVRSILFRVAEDEFIMALVAGPAQISWKTLRKHLGKSRVSMASEDEVLAVTGYRIGAV
                     GPFGLLNQVKVLIDPSVMLEEEISIGSGMRNTAVILKSADLHHALKNSEIVALTE"
     gene            521..1360
                     /locus_tag="PLE39_18535"
     CDS             521..1360
                     /locus_tag="PLE39_18535"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HNE70388.1"
                     /translation="MSSFKQRTLDFLEIEWASYIQRFERWPADEGLKRVNAQGYEQFR
                     DMLAHVLAWWEEGMGIILAIAEDREYARKKYDFDAFNAEAVAKYKGWNEAQFLAHFEK
                     TRQKTVADLRSMNKAAWENRRVQAWVNGIFINHAREHLVASSRFLILDTLEHGWGTYI
                     EDFEKVEDKAAFLSKQGVENFRELLGHAIGWWEEGERIIKGILSDPNFTWQDRDTDAF
                     NRELIVKYNQLSDEEVQKQFESKRQDMIRFVKGLPDSAFTNKDIEGWLAADIAEHYDE
                     HRP"
     gene            1388..1867
                     /gene="folK"
                     /locus_tag="PLE39_18540"
     CDS             1388..1867
                     /gene="folK"
                     /locus_tag="PLE39_18540"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNE70389.1"
                     /translation="MNHIVYLALGSNMGNRMMNLKTAISNLTPQMTVKQKSSVYETPP
                     WGFTEQAAFLNQVLKVETYLKPEALIAHLKRLESALGRVPNFTNGPRLIDIDILFFDD
                     MVIYTPPLVVPHPRLHERAFVLLPLAEIAPDLIHPVLHKSVSELLDDAERIEIKIVK"
     gene            1938..2624
                     /locus_tag="PLE39_18545"
     CDS             1938..2624
                     /locus_tag="PLE39_18545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNE70390.1"
                     /translation="MDIEVVHRPSYSLAIAKLTPNERIRAEAGAMVSMSEGVQIETKA
                     EGGFLKSLGRAVLGGESFFQNFFVAPPQGGEVTLAPELPGDITVIEMSGGKLMIQSGS
                     YMASESTVELTAKVSVKALMSAEGVSMLEATGTGTLLVSSYGAIYEKNLNAGEKYTVD
                     TSHLVAFDGNMGVTPKPIGGLKSTLFSGEGFVIELSGPGKIYIQTRSPQALINWIIPQ
                     LPKPPSSSNH"
     gene            2768..3526
                     /locus_tag="PLE39_18550"
     CDS             2768..3526
                     /locus_tag="PLE39_18550"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNE70391.1"
                     /translation="MKIVRYQTNGEAVKYGWMLDDKVGEIQGDIFGEYRRREAKIPVG
                     ELKLAAPCVPSKILCVGRNYVEHAKELGNEVPKVPLIFLKPPSSIISNGEHIVLPPQS
                     SQVEHEAELVVVIGKRGRNITIENAKEYVFGYTIGNDITARDLQKTDGQWTRAKGFDT
                     FCSFGPWIDTEFDASDAVVTCRVNGQMRQMASTRDMVFNINTLIAFISSVMTLEAGDI
                     IFTGTPSGVGELKNGDMVDVEIEGLGKLSNPVKS"
     gene            3603..>4972
                     /locus_tag="PLE39_18555"
     CDS             3603..>4972
                     /locus_tag="PLE39_18555"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE70392.1"
                     /translation="MTTNELETFLAQYPTYKNTEAIDKLRKSDYARLDAGEHVYLDYT
                     GGGIYAESQIQKHHKLLNENVYGNPHSSNPTSQAATHLVEGTREYILKFFSADPDEYL
                     AIFTPNASGALKLVGESYPFPNGRYLLTFDNHNSVNGIREFAHARGAEVTYIPVMLPE
                     MRIDASQLDLELARPSKNGYNLFAYPAQSNFSSVKHPLEWIEKAHAHGWDVLLDAAAY
                     VPTNKLDLSKVKPDFVPISFYKIFGYPTGLGALIARKSALAKLHRPWFAGGTITVASV
                     QGDKYYLADGAPAFEDGTLDYLNIPAIEIGLKHIESIGYDSISERVRTLTGWLLQNLT
                     AMKHSTGEPLVRLFGPATSEGRGGAVTVNFYDKNGKAFDHRFIESEANKVNISLRTGC
                     FCNPGAGEVALGISRVELDVCFTSPDHKDKMSIDDFRLCIDGKSSGAVRISVGMVTNF
                     NDVQAF"
BASE COUNT         1252 a         1319 c         1306 g         1095 t
ORIGIN      
        1 atggaaactc ccccagtcag tattgctttg gaacaacttg gtgtgccgca cacaatcttt
       61 cggcacgatg acgcgcgtcc tgtccactcg ttggagcagg cggcaagtga ccgtggtcag
      121 cgggcttcgc aggtggtgcg gagcattttg ttccgtgtgg ccgaggatga atttataatg
      181 gctctggtcg ctggccccgc gcaaatctca tggaagactt tgcggaagca ccttggaaaa
      241 tcccgcgttt cgatggcctc agaggatgag gttctggcgg tgacgggcta ccgaatcggg
      301 gcggtggggc cgttcggatt gctgaatcag gtcaaggtgt tgatcgatcc gagcgtgatg
      361 ctcgaagagg aaatttccat cgggtcgggg atgcgcaata cggcggtcat cctcaaaagc
      421 gcggatctgc atcacgcttt gaaaaattcc gagatcgttg cattgaccga gtagggcccg
      481 tttcatcagg gcgaatccat ttataaaaac ggagaacatc atgtccagtt tcaaacaacg
      541 cacacttgat tttcttgaga tcgaatgggc aagctatatt cagcggtttg agcgctggcc
      601 tgccgatgaa ggcttgaagc gggtcaatgc gcagggctat gagcagttcc gcgatatgct
      661 ggcccatgtg ctggcatggt gggaagaggg catgggcatc atcctcgcca ttgcagagga
      721 ccgcgagtat gcgcgcaaga aatatgattt tgatgcattc aatgccgaag cagtggcaaa
      781 gtacaaaggc tggaatgaag cgcaattcct cgcgcatttt gaaaaaacgc gccagaagac
      841 cgtggctgat ttacgttcca tgaacaaggc cgcgtgggaa aatcgccgtg tacaggcctg
      901 ggtcaatggt attttcatca atcacgcccg tgagcatctg gtggcttcga gccgtttttt
      961 gatcctggat acgcttgagc atggatgggg cacatacatt gaagactttg agaaggtcga
     1021 agacaaggct gcatttctga gcaaacaggg tgtggagaat ttccgtgaat tgctgggtca
     1081 tgccattggc tggtgggagg aaggcgagcg catcatcaaa ggtattttga gtgacccgaa
     1141 tttcacatgg caagatcgtg acaccgatgc cttcaacagg gaattgatcg taaaatacaa
     1201 ccagctttca gacgaagaag tgcaaaaaca atttgaaagc aaacggcagg atatgattcg
     1261 attcgtgaaa ggattgcccg actcggcttt tacgaacaag gatatcgaag gctggctcgc
     1321 ggcggatatc gccgaacatt acgacgaaca caggccataa tccatttttc agattgacaa
     1381 ggcaggcatg aatcacattg tttatctcgc ccttggaagc aacatgggca accgcatgat
     1441 gaacctcaag acagcgatca gcaacctcac gccgcaaatg accgtgaagc agaaatcgtc
     1501 tgtgtatgaa acgccgccat ggggattcac cgagcaggcc gccttcctta atcaagtgct
     1561 gaaagtggag acgtacctca agccagaggc attgatcgcc catttgaaac ggcttgaatc
     1621 ggcattggga cgtgtgccca actttacaaa tggcccgcgc ctgatcgaca ttgacatcct
     1681 gttcttcgat gacatggtga tctacacgcc cccgctggtg gtgccgcatc cgcgcctgca
     1741 tgaacgcgcc tttgtgcttt taccgctggc agagatcgcc ccggatttga tccatcctgt
     1801 tttgcataaa tctgtcagtg aattgctgga tgatgcggaa cggatcgaga taaaaattgt
     1861 aaaatgatct tgtagagcgt cataggtggc gcgacctaac atgtcgcgct acaacaaaac
     1921 ccaaaggaga tttacacatg gatattgaag tcgttcaccg cccgtcgtat tctcttgcaa
     1981 ttgcaaaact cacccccaat gaacgcatcc gcgccgaggc tggggccatg gtcagcatgt
     2041 cggaaggtgt gcagatcgaa accaaggctg aaggcggatt cctcaaatcg ctcggacgtg
     2101 ccgtgttggg cggcgaatcc ttcttccaga atttcttcgt agctccccca cagggcggcg
     2161 aagtgaccct tgccccggaa ttacccggtg atatcaccgt catcgagatg agcggcggaa
     2221 aattgatgat tcaatcgggt tcctacatgg cctctgaatc aacggtggaa ttgaccgcca
     2281 aagtgagcgt gaaagccttg atgtctgccg aaggcgtttc catgcttgaa gccacgggaa
     2341 cgggtacgtt gttggtctct tcctacggag cgatctacga aaaaaacctg aatgcaggcg
     2401 aaaaatacac ggtggatacc tctcatctcg tggcgtttga cgggaacatg ggtgtcaccc
     2461 ccaaacccat cggcggactc aaatccacgc tgtttagcgg cgaagggttt gtgatcgagt
     2521 tgagcggccc gggcaaaatc tacatccaga cccgctcgcc gcaggcattg atcaattgga
     2581 tcattccgca gctgccgaag ccgccatcct cctccaatca ctaaatcgcc gttggcttgt
     2641 ataatagatg cagggcaaac ccagtttgtc ctgcattttt gttttgcaaa acacctgcac
     2701 cgcacccaaa ggcggtgagc agcacaaagg gtttcctttg tagatttcgt tttgacacag
     2761 gagaagcatg aaaatcgttc gttatcaaac caatggtgag gctgtcaaat acggctggat
     2821 gcttgatgat aaggtcggcg agatccaggg cgatatcttc ggagaatacc gccgccgcga
     2881 ggcaaagatt cccgttggag aattaaaact ggccgccccc tgcgtaccga gcaagatctt
     2941 atgcgtgggg cgcaactacg tggaacacgc caaggaactg ggcaacgaag ttcccaaagt
     3001 gccgctcatc tttttgaaac cgccctcctc gatcatctcg aacggcgaac acatcgtcct
     3061 gccgccgcaa tccagccagg tggagcacga agcggaattg gtcgtcgtca tcggcaaacg
     3121 cggacgaaac atcaccatcg aaaacgcgaa ggaatacgtc ttcggctaca ccatcggcaa
     3181 cgacatcacc gcgcgcgacc tgcaaaaaac agacggtcaa tggacccggg ccaaagggtt
     3241 cgacaccttc tgttctttcg gcccgtggat cgacaccgag ttcgacgcct ccgatgctgt
     3301 ggtgacctgc cgtgtgaacg gccagatgcg ccagatggca tccactcgcg atatggtttt
     3361 caacatcaac accttgatcg ccttcatttc ttcagtgatg acactcgaag caggcgatat
     3421 tattttcaca ggcacgccat cgggtgtggg cgaattaaag aatggcgaca tggtggatgt
     3481 ggagatcgaa ggtctcggca agctaagcaa ccctgttaaa tcgtaggtca ggcttcctgc
     3541 ctgacttttt gaccacaaaa catggcgggc tgcaaacccg ccctacaaaa caggaaccca
     3601 ttatgaccac aaacgaactc gaaacttttc ttgcgcagta ccccacctac aaaaacaccg
     3661 aagccataga taaacttcgc aaatcagatt atgcccgcct cgatgcaggc gagcatgttt
     3721 atctcgatta cacgggcggc ggcatctacg ccgagtcaca gattcaaaag catcacaaac
     3781 tcttgaacga aaatgtctat ggcaacccgc attcatccaa ccccacctca caggcggcca
     3841 ctcaccttgt tgaaggcacg cgcgagtaca tcctcaaatt cttcagcgcc gacccagacg
     3901 aatatcttgc cattttcacc cccaatgcca gcggcgcact caaactcgtc ggcgaaagtt
     3961 atcccttccc gaacgggcgt tacttgctga ctttcgataa tcacaattcg gtgaatggga
     4021 ttcgggaatt tgcacacgcc cgtggtgcgg aagtgaccta cattccagtc atgctgcctg
     4081 aaatgagaat cgacgcttca caactggacc tggaactggc acggccttcg aagaatggat
     4141 acaacctttt tgcatatccc gcgcaatcca acttctcctc ggtcaaacat ccgctggaat
     4201 ggatcgagaa agcccatgcc cacggttggg atgtgctgct ggatgccgcc gcctacgtgc
     4261 ccaccaacaa actcgatctg agcaaggtca agcctgattt tgtgccgatc tctttctaca
     4321 agatcttcgg ctaccccaca gggctgggcg cattgatcgc acgcaaatct gcgctggcaa
     4381 aattgcaccg cccctggttt gcaggcggaa ccatcacggt tgcctcggtg caaggcgaca
     4441 aatattacct cgccgacggc gctcccgcct ttgaagatgg cacgcttgat tacctcaaca
     4501 tccccgccat cgagatcgga ctcaagcata tcgaatccat cggatacgat tcgatcagtg
     4561 aacgggtcag gacgctaaca ggctggctgc tgcaaaacct gaccgccatg aaacacagca
     4621 ccggggagcc gcttgtccgt ttatttggac cagccaccag tgaaggaaga ggcggcgcgg
     4681 tcacggtcaa tttttacgac aagaacggca aagccttcga tcatcgtttt atcgaaagtg
     4741 aagccaacaa ggtcaatatc tcactgcgga caggctgttt ctgcaatccc ggtgcaggtg
     4801 aagtggcgct cggcatctcc cgtgtggaat tggatgtgtg cttcacctcc cctgatcaca
     4861 aagacaaaat gagcattgac gacttccgcc tgtgcattga cggaaaatcc tcgggcgctg
     4921 tgcgcatctc tgtgggcatg gtcacaaact tcaacgacgt gcaagccttt tt
//
LOCUS       DAOQJC010000204         4953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_10041_length_4953_cov_5.939976, whole genome shotgun sequence.
ACCESSION   DAOQJC010000204 DAOQJC010000000
VERSION     DAOQJC010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10041_length_4953_cov_5.939976"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PLE39_18560"
     CDS             <1..506
                     /locus_tag="PLE39_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70393.1"
                     /translation="AQRIGLIGGSQAGWLIPIAASESEHVSFFVILSGPVASTLQEDI
                     FSAYTDDGESSGDYDDAEITRKLRSLGFGGFNPIPYLAELTQPGLWLWGSVDKSVPVT
                     FSRENLQVLIDGGKNNFEYQVLAGGDHGLSITPNGLFSEMPYSPGLALYGPLTEWLEK
                     NNISAKE"
     gene            511..1599
                     /locus_tag="PLE39_18565"
     CDS             511..1599
                     /locus_tag="PLE39_18565"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70394.1"
                     /translation="MKKHLMVFLTFILMACAAPAAEVSPTEGAAPLATVTAIPPTETA
                     EPQTFFKVFGDAPIVSKGETGTWDDRYTDPGAVVFHDGIFHMFRNGFRGFPAESQVGY
                     VTSVDGYTWEKQGSDPVFLTKDVPYAQVAMFVSSALVEDDGTWVLYFYTLDSVNFPFS
                     GVIGRATAASPNGPWIPDAEPVLMPGSAREWDSRNVAAPHVIKTADGYVMYYSGTDST
                     GKVQVGMAASSDGVQWAKYNDPATTEIQYQESDPVLLLGEKSSWESDQVHQARVFPDG
                     DGWVMIYRGSNKNQASMGLGIATSRDGIHWAKSALNPVLLPTEIKGAYQFWFHNALFV
                     NGTYFVFIEGDINQRTQIYLATREGEMP"
     gene            complement(1724..2269)
                     /locus_tag="PLE39_18570"
     CDS             complement(1724..2269)
                     /locus_tag="PLE39_18570"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNE70395.1"
                     /translation="MSSTKAPTRHHPALVTLHWLVFILIFALSVSGTYAAGLANDDPA
                     KRSILILHLPFGSTTFFLLIASLVVHLRTPRPAYASTGNSILDTIGKITHVALFVLAL
                     LTSMSGMFLSIRSGLMPTIFLNASAALPADFFIFIERIFHGYVSKALFFLVLLHIGAA
                     LYHQVWLKDNLFSRMGYGKNL"
     gene            complement(2339..3160)
                     /locus_tag="PLE39_18575"
     CDS             complement(2339..3160)
                     /locus_tag="PLE39_18575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE70396.1"
                     /translation="MQQAMLNDFSTAHTVSAAIKTNWLNYHYAMWQSPKVELSISPYL
                     NWLITDIPDYFLNLVVCTQLPEHGIETLVEKAMNHFEKNNVPRFMWFVEEGVQAENLK
                     HHLAARGLTFRESFATKMAAHLENLSENISMPKGLEIVPVENRTTLKQWIHLTSRGFG
                     IPAEYEDTWCDIFDNTVFNAPFRTYLGVLNGQPVGTAQLFTSAGVAGIYNITCAPEAR
                     CQGVATALTKTALLDARQLGYRVSVLQASQAGAKVYRKLGFQDYGNLSAFMWKRN"
     gene            complement(3249..3518)
                     /locus_tag="PLE39_18580"
     CDS             complement(3249..3518)
                     /locus_tag="PLE39_18580"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNE70397.1"
                     /translation="MSKLIIRHKVQDYSVWRPGYDGHAAARAAAGCLSAQVFQSANDS
                     NEITVIMDWPSVETAQAFASSPDLKEVMKNLGVISQPDVSFLVEA"
     gene            complement(3680..>4953)
                     /locus_tag="PLE39_18585"
     CDS             complement(3680..>4953)
                     /locus_tag="PLE39_18585"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70398.1"
                     /translation="LTMQVTLKDMSDARRFAHHRQVGETLESLYRENLDAVSGLIAHH
                     FAEGNLPARAASYAFRAGQSAALLAAWVEAIAFYEQAISLERDDAQRAPMYLALSEAH
                     FHKGDFALAAADCRTAIPLARANADWRMVENISLQLNKSLIPQALYSETIALGNELLE
                     LGIPELGICAKTILGVGLSHESAHPVEAERHLREAERLLHQQTEYSGPVSLAVIEYHL
                     ASAISQQGRTAEAIALFRKVLELLEQGKSTLDLIRHILIYNNLAYQLYLVGDPSAKEY
                     IQVGIKLAHEKRSLSHLPYLYSTSGEIALANHELDAAEAFFRDGLALAEKIPLPESIA
                     GLNANLGLVAKERGDSTLAHEQLKNALTLAEQLGNRHLEVRVRIWLAPLQPPAEALPS
                     LEIAHTFAVQGGLGNLLEEIIGLQNSLSNPL"
BASE COUNT         1221 a         1145 c         1343 g         1244 t
ORIGIN      
        1 atgcgcagcg catcggtttg atcggcggaa gtcaggcagg gtggttgatc ccgatcgctg
       61 catctgaatc tgaacatgtt tctttctttg taattctttc aggccctgtg gcatccacac
      121 ttcaggaaga tatctttagc gcctacaccg atgacggtga atcttcgggg gattatgacg
      181 atgcagagat cacccgtaaa ctgcgcagtt tgggcttcgg cgggtttaac ccgattccat
      241 accttgctga attgacacaa cccggcctgt ggctatgggg cagtgtggat aaaagcgtgc
      301 ctgtcacttt cagcagggaa aatttacagg ttctgatcga tggcggcaaa aacaattttg
      361 aatatcaagt ccttgctggt ggagatcatg gtctgagcat cacccccaat ggtctcttct
      421 cggaaatgcc ttattcgcca gggctggcgt tgtatggtcc tttgacggaa tggcttgaaa
      481 aaaataatat ctctgcaaag gaatagttca atgaaaaaac accttatggt ttttctgaca
      541 tttattttga tggcctgcgc cgcccctgct gcggaagtga gccctaccga aggcgccgcg
      601 ccgcttgcaa cggtcactgc aattccgccc actgaaactg cggagccgca gacatttttt
      661 aaagtatttg gcgatgcgcc cattgtttcc aaaggtgaga caggtacctg ggatgaccgc
      721 tacaccgacc ctggcgctgt ggtcttccat gacggaattt ttcacatgtt tcgcaatggc
      781 ttccgcggat tccccgctga atcacaggtg ggctacgtca cttcggtgga tgggtacact
      841 tgggagaagc agggaagtga ccccgttttt ctgacaaagg atgtgcccta tgcccaggtg
      901 gcgatgtttg tttccagcgc gctggtggaa gacgatggga cttgggtgtt gtatttttac
      961 acgcttgatt cggtgaactt tccctttagt ggggtgatcg gacgggcaac ggcggccagc
     1021 cccaacggcc cctggatacc cgatgccgag cctgtgctca tgccagggtc ggcgcgtgaa
     1081 tgggattcga gaaatgttgc cgcgccgcac gtcatcaaga cagcggatgg gtatgtgatg
     1141 tattacagtg gaactgacag cacgggtaag gtgcaggttg gcatggctgc ttcgagtgat
     1201 ggagttcaat gggcgaaata caatgaccct gccacgacag agatccaata tcaagaaagc
     1261 gaccccgttt tgctgcttgg tgaaaaaagc agctgggaat cagaccaggt gcatcaggcg
     1321 cgtgtctttc cagatggaga tgggtgggtg atgatctacc gcgggtcgaa taagaatcaa
     1381 gccagtatgg ggttgggcat cgccaccagc agggatggaa tccactgggc gaagtctgcg
     1441 cttaatcccg tccttttgcc caccgagatc aaaggcgcat atcaattctg gttccacaat
     1501 gcactgtttg tgaatggtac ctattttgtt tttatcgaag gcgatattaa tcaacggacg
     1561 caaatttatt tagcgacgcg tgaaggcgaa atgccataaa tgaaaatgga cggtagatag
     1621 tggcttttca aaaagaagca ctatctgccg tccatcaaaa caagatgcgg tcatctttgt
     1681 tcaagcagga tattgaggta ccctgaaaga tatgcttgag actttacaaa ttttttccat
     1741 accccatgcg cgaaaacaga ttgtctttta accaaacctg atgatagagt gcggctccaa
     1801 tatggagtaa gaccaggaaa aacaatgctt ttgaaacata gccgtgaaag atacgttcga
     1861 taaagataaa aaaatctgcg ggcagggcgg cgcttgcatt gaggaaaatg gttggcatca
     1921 gacctgagcg tatagataag aacatgccgc tcatgcttgt caacaacgcg agtacgaaca
     1981 gcgcaacatg ggtgattttt ccaattgtgt ccaaaatcga gttgcccgtt gaagcataag
     2041 ctggcctggg tgtacgcagg tgaacgacaa ggctggcgat taacaagaag aatgttgtac
     2101 ttccaaatgg gagatgaaga atcaggatac tccttttggc tgggtcatca tttgccagac
     2161 cagcagcgta tgtgccgctt acagataaag caaagatcag gatgaagacc agccagtgta
     2221 gggtgaccag tgctgggtga tgacgggtgg gggcttttgt agaagacatg tcggattcct
     2281 tttgatgtga tattttagag cgtgaatata gctttgaaga aaataaaaaa tatggcatct
     2341 aattgcgctt ccacatgaag gcgctcaagt tcccgtagtc ttgaaaacct agcttacgat
     2401 acaccttggc gcctgcctgg gatgcctgta agacactgac acgataaccc agttgacgtg
     2461 catcgagtaa ggcggttttg gttagagcgg tcgctacacc ctggcatctg gcttcgggtg
     2521 cgcaggtgat gttgtagatt cctgccacgc ctgcggaggt gaaaagctgg gcggtgccga
     2581 cgggctgccc attcaaaacg cccaaataag tgcggaaggg tgcattgaat accgtgttgt
     2641 caaaaatatc gcaccaggtg tcctcatact ctgctgggat tccaaagccg cggcttgtga
     2701 gatggatcca ctgcttgagt gttgtgcgat tctcaacggg aacgatctcc aggcccttgg
     2761 gcatggaaat gttttctgac aggttttcca aatgggctgc cattttggtg gcgaaggatt
     2821 cgcggaaggt caatccgcga gccgccaggt ggtgtttcag gttttctgcc tgaacgcctt
     2881 cttcgacaaa ccacataaac ctggggacat tgtttttctc gaagtgattc atggcttttt
     2941 caacgagagt ttcaatgccg tgttcgggca actgcgtgca gacgactaaa ttcaaaaaat
     3001 aatctgggat gtcggtgatc agccagttca agtaaggcga gatggataat tcaactttgg
     3061 gcgattgcca catggcataa tggtaattca gccagttggt cttaatggct gcgctgactg
     3121 tatgtgccgt tgaaaaatca tttagcatcg cctgttgcat ggggatatac ctctacgatc
     3181 ggtcttgcaa acaaaaagga tttacacata tggagtatgc gtaaatcctt ttcatggttg
     3241 aatacagctt aggcttcgac caggaagctg acatctggct gagagatgac gcctaagttc
     3301 ttcatcactt ccttgaggtc tggggatgaa gcaaatgctt gggcagtttc caccgaaggc
     3361 caatccataa ttacggtgat ctcattggag tcattggcag attggaagac ctgtgcgctt
     3421 aggcaacctg ctgccgcgcg ggcggcggca tgaccgtcat accctgggcg ccaaactgag
     3481 taatcctgca ctttgtggcg aataataagt tttgacattt tatttctcct tgtgtgagtt
     3541 ggtttggtat ggagcatgct ccacatgttt atcttgaccc aatcatacaa aggcggcgtt
     3601 aagcgaacgt caacaggagc gttaaatcat ccgcagggtt tgaagaaccc tgcggatgat
     3661 ttttttacga tgaatatgat tacaaaggat tggaaagact gttttgcagc ccgatgattt
     3721 cttcgagcag attgcccaac ccgccctgca ctgcaaaggt gtgggcaatt tccaaactgg
     3781 ggagcgcttc tgcaggaggc tgaagcggcg ccagccaaat tcggacacgg acttcaaggt
     3841 gtcgattccc caattgttct gccagagtca acgcgttctt gagttgttca tgcgccagcg
     3901 tagagtcgcc ccgttctttg gcaaccagcc ccaggttggc gttcagaccc gctatcgatt
     3961 caggcagagg aattttctca gcgagagcaa gtccatcgcg gaaaaacgct tcggcggcat
     4021 ccagttcatg attggcaagc gcaatttcac ccgaggtgga atacagatac ggcaggtgcg
     4081 agagcgatct tttttcgtgg gcaagtttga taccaacttg aatgtattct ttcgcggagg
     4141 gatctccgac cagatacaat tgatacgcca ggttgttgta gatcaggata tgcctgatca
     4201 aatccagcgt gcttttacct tgttccagta gttcaagcac cttgcgaaag agggcaatcg
     4261 cttctgccgt acgcccctgc tgactaatgg cgcttgccaa atgatattcg atgacggcta
     4321 aagagaccgg acctgaatac tccgtttgtt ggtgcagcaa gcgttctgct tcgcgcagat
     4381 ggcgttccgc ttctacgggg tgcgcgcttt catggctcaa ccccaccccc aaaattgtct
     4441 ttgcgcaaat tcccaactca ggtataccca gctcgagcaa ttcatttccc aaggcaatcg
     4501 tttcagagta aagggcttgc ggtatgagag atttattgag ttgcaaactg atattttcca
     4561 ccatgcgcca atcggcattc gcacgtgcca acgggatggc ggtgcggcaa tcggcagctg
     4621 ccaaagcaaa atcgccttta tggaaatgag cctcgcttaa cgcaaggtac atgggagcgc
     4681 gctgcgcatc atctctttcg agtgagattg cctgctcgta aaaagcaatt gcctccaccc
     4741 aggcagccag cagggcagcg gactgacccg cgcgaaacgc atacgaagca gcacgggcgg
     4801 gcaggtttcc ttctgcaaag tgatgtgcga tcaaaccaga gacagcatcc aggttttcgc
     4861 ggtaaagact ttcgagcgtc tcgccgacct gtcggtgatg ggcaaagcgg cgggcatcac
     4921 tcatatcttt gagggttact tgcatggtta agg
//
LOCUS       DAOQJC010000205         4940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_10069_length_4940_cov_10.305629, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000205 DAOQJC010000000
VERSION     DAOQJC010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10069_length_4940_cov_10.305629"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PLE39_18590"
     CDS             complement(<1..1115)
                     /locus_tag="PLE39_18590"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNE70399.1"
                     /translation="MRIISLTLILLCLLTACTSPTAIPTPIPSPTTTPPPLTGEWIGT
                     AAKPDGSIASVIVNFDEDIPELNIEPMTRRWKLELKQEGGQLRFLAEGRTRDPFERIE
                     FSGRAENGLISGEITWDGHTSQTHFLQLAAVTDEILASYEGVYRFDSGRTLSIIVSPE
                     FSSSGLEYFGKSLMMTDFENGNLRSLYAVDDSTFLVGVLRVVGAPFDGRVEFMKDGQG
                     NIEGLMWWQTPNDILSSPVPSEFAARVPFVMDDGVSYFSADGTKLVGRLTMPSSAGPH
                     AAFVMPHGSEAGTRDNFGAKLTAHYFLSRGIATLNYDKRGVGDSEGIYHESADSTNLK
                     NLAADANAGVEYLLTRSEIDAQRIGLIGGSQAGWLIP"
     gene            complement(1174..3870)
                     /locus_tag="PLE39_18595"
     CDS             complement(1174..3870)
                     /locus_tag="PLE39_18595"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNE70400.1"
                     /translation="MTVPAMIPTKINIPRPRMDVVVRPRIFSILDEGWAAPTALTLIS
                     APAGSGKTTLLVNWLQNKKHKAAWFSLGDEDDLFPRFVSYVIAALQSLDPVLGRSAAK
                     AFTSGDGQTPQSEAVINSLLRDCAVLEHPVALVLEDYHFIKSAEIHRLVTEIAEYLQP
                     YLRLFITTRNDPPLPLAKWRVRHQLVEIRSSDLQFTAQETGEFLRRVMKVDLPAAELT
                     NLQQQTEGWAAGLQLAALSLRSRKQQGIAWSWGDGNRDLSDYLMAEVLGQLPPEKQDF
                     LMQTALVTRFSAALCNTLTGGNNAQAILDELDSENLFLVSLDDSREWFRFHHLFADFL
                     QKQALIRYPAEAIRGLRQRAARWLAGQGYVTEAIEHALAAQDYEFAAALIGPQSQEWM
                     RRGEVATILEKMKQLPDEIVSKNAGLCIWYGWVYALGDSPQLAERWSDRAEAVLSPDL
                     KTVATDPEQFGPGIRNSHAQILAIRATAARHRHDFQTAVQLAEQALRIVPKGNTHLQM
                     IISAGLSTSQIEVGDFAQADSVTNATRQLAYQTGHPFIEFSMLINEAVLAMARGQLYK
                     AHQVAEDALHLADAESMQHLAFIPHGYLGEVNFLWNKLNEARQHILQGNHNLNFKEYP
                     TSSVRAYLNLAMLHQAEGEFAQALDALEKARRFAQKHQVADLFERAKGLQALYQFSAG
                     KTEAVERWAKSSGWDSFDPVQSKLLFNEESFYAACRYRIDLAKPTEYQKVETLLEWCL
                     GEAEAQQRVGVILKVRLLQVCLYQAQGESDAALAALVQALTLAEPEKFIRPFLDAGEL
                     IESLLPRVPHSHSARAFAQTILTNFHTQKPQPMPVLLEPLNEQELGILRLMAQGFSNP
                     EIAQKRALAVSTVRWYVKHIHRKLGVHNRTQAAARAREMDLL"
     gene            complement(3968..4348)
                     /locus_tag="PLE39_18600"
     CDS             complement(3968..4348)
                     /locus_tag="PLE39_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70401.1"
                     /translation="MDPRLEQDALIEDALKSQPLAPMPRSITANVMSRIQAVPSQQRP
                     ALVTWNDFVLSLFIALSIGAVLFALQSLPPVAVVKLQIQWILLYQDFIVNARWLLPAL
                     SFGLAAFFAALTIPTLVQMVSNRQ"
     gene            complement(4333..4899)
                     /locus_tag="PLE39_18605"
     CDS             complement(4333..4899)
                     /locus_tag="PLE39_18605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE70402.1"
                     /translation="MVISEQILVQKAQKGDVQAFEALVNEHQQYVYNLALRVVKDENE
                     ALDLAQETFVRAWTALPNFKGQSQFRTWLYRIVTNLCYNRLPNLRRSLNDLGDDMMED
                     IPEPYLDTPAQIFEVSETRKHLQDAIQSLDSNYQLLITLRYQNELSYEEIASTLNLPL
                     GTVKTGIFRAKEQLRKSLAQLEESWIPA"
BASE COUNT         1199 a         1284 c         1302 g         1155 t
ORIGIN      
        1 atcgggatca accaccctgc ctgacttccg ccgatcaaac cgatgcgctg cgcatctatt
       61 tctgaacggg taagcagata ctcgaccccg gcatttgcat cggctgccag atttttcaaa
      121 ttcgtagaat cagcgctctc atgataaata ccctcagaat cgcccacacc gcgcttgtca
      181 taattcaaag tggcaattcc gcggctgagg aaataatgcg ccgtcaattt cgcgccaaaa
      241 ttatctcgtg tccccgcttc agagccgtgc ggcatcacaa aggctgcatg cggccccgct
      301 gacgatggca tggtgagtcg ccccaccagc ttcgttccat ccgcactaaa gtagctgacg
      361 ccgtcatcca tcacaaatgg gactcgcgcc gcaaactctg aaggcacagg tgaagagaga
      421 atatcattcg gggtctgcca ccacatcaaa ccttcaatat tcccctgccc gtctttcatg
      481 aattcgactc tgccgtcgaa gggggcgccc accacgcgaa gcaccccaac caaaaaagtg
      541 gaatcatcca ctgcatacaa actgcgcagg ttgccgtttt cgaaatcggt catcatcagc
      601 gatttgccga agtattcaag cccgctggat gagaattcag ggctgacaat gatgctcagg
      661 gtacgccctg aatcaaaacg atagacgcct tcatagctgg caagaatttc atctgtcaca
      721 gcagccagtt ggaggaaatg ggtctggctt gtatgcccat cccaggtgat ctcgccggag
      781 atgagtccat tttccgccct gccgctgaat tcgatcctct cgaacgggtc gcgggtgcgg
      841 ccttctgcca aaaagcggag ttgcccgcct tcctgtttca attccaattt ccacctgcgg
      901 gtcattggct caatattcag ttcgggtatg tcttcatcaa aattcacaat gaccgatgca
      961 atggagccat cgggcttggc ggcggtgccg atccactccc ctgtcagtgg cgggggtgtt
     1021 gtagttgggc ttggaatggg tgtgggaatc gctgtcgggc ttgtacaagc cgtcaaaagg
     1081 cagagcagga tcaaggtcag ggatatgatg cgcatggtca caatatatcg gcgcatccac
     1141 gaaaagtcgc cctccaattg gggggaaaat caattaaaga agatccatct cacgtgcccg
     1201 tgctgccgcc tgcgttcgat tatgcacccc aagttttcga tggatgtgtt tgacatacca
     1261 gcgcacagtg ctgaccgcca gcgcgcgctt ctgtgcgatc tcaggattgg aaaatccctg
     1321 tgccatcagc cgcaagatcc caagttcctg ttcgttgagc ggttcgagca gcacaggcat
     1381 tggctgcggt ttttgggtat gaaaattggt caaaatggtt tgcgcaaatg cgcgtgcaga
     1441 atgtgaatgc ggcacacgcg gcaaaagcga ctcgatcaac tcgcccgcat cgagaaaagg
     1501 gcgaatgaat ttttcaggct cagccagtgt caatgcctga accagggcgg ccagtgccgc
     1561 atcactctct ccctgtgcct gatacaaaca aacttgcaac agacgcactt tgagaatcac
     1621 acccacccgt tgttgtgcct cagcctcgcc gagacaccat tcgagcagcg tttccacttt
     1681 ttgatattcc gtcggctttg ccagatcaat tcgataccga caggctgcat aaaacgattc
     1741 ttcattaaac agcagcttcg actgaacagg atcaaaggaa tcccaaccgc tggattttgc
     1801 ccaacgttca accgcctcgg tcttccccgc tgaaaactgg tacagcgcct gcaaaccttt
     1861 tgcccgctca aacagatctg ccacctgatg tttttgcgca aaacggcgcg ccttctcaag
     1921 agcatccaac gcctgtgcaa attctccttc agcctgatgc agcatggcaa gattcagata
     1981 agccctgaca ctgctggttg ggtattcctt gaaattcagg ttgtgattcc cctgcaaaat
     2041 atgttgtcgc gcctcgttca atttattcca aagaaagttc acctcgccaa gatacccatg
     2101 cggaatgaag gcaagatgct gcatggattc ggcatcggca agatgcaggg cgtcttcagc
     2161 aacttgatgc gctttataca attgtccgcg cgccatggcc agcaccgctt catttatcag
     2221 catggaaaac tcgatgaagg gatgccctgt ttggtaggcc aactgtcgcg tagcgtttgt
     2281 tacagaatca gcttgtgcaa aatcacccac ctcgatctgc gaagtggata agccagccga
     2341 tatgatcatc tgcaagtggg tgttgccttt gggcacgatc ctcaaagcct gttcagcaag
     2401 ttgcaccgca gtttgaaaat catggcgatg ccgtgcggca gtggcacgaa tcgccagaat
     2461 ttgtgcatgg gagttgcgaa taccggggcc aaattgttca gggtcggttg caaccgtttt
     2521 caggtcaggt gaaagaaccg cttcggcacg gtctgaccat cgttcggcca gttgcggcga
     2581 atcaccaagc gcgtacaccc atccatacca aatacacaac cctgcattct tgctgacaat
     2641 ttcatcaggt agttgtttca ttttttcaag gatggtggca acttctccgc ggcgcatcca
     2701 ctcttgcgat tgcgggccga tcaatgcagc cgcaaattca taatcttgag cggcaagggc
     2761 atgttcgatg gcctcggtca catatccctg ccctgccagc cagcgggcgg cgcgctgacg
     2821 caggcctcga attgcctccg ctgggtatct gatcaatgcc tgtttttgaa ggaagtcagc
     2881 aaacagatga tgaaaacgaa accattcacg tgaatcgtcc agtgaaacga ggaagagatt
     2941 ttctgaatcc agttcatcca aaatggcttg ggcgttgttt cccccagtca gtgtgttgca
     3001 caaagcggcg ctgaaacggg ttacgagcgc ggtctgcatg agaaaatcct gtttttcggg
     3061 cggcaattgc ccgagcacct cggccatcaa ataatcggaa agatcacgat tgccatctcc
     3121 ccatgaccag gctattccct gctgttttcg gctgcgcagg gatagggcgg ccagctgcaa
     3181 accagccgcc cagccttccg tctgttgttg aaggtttgtc aactcagcag cagggaggtc
     3241 aactttcatc acacggcgta aaaattcacc cgtttcttgt gccgtgaact gcaaatcgct
     3301 ggagcgaatt tcaacaagtt gatgacgtac ccgccatttt gccagcggca ggggtggatc
     3361 attacgggtg gtaatgaaca accgtagata cggctgcaag tattcagcga tttcagtcac
     3421 caaacggtga atctcggcgg atttgataaa gtgataatct tccaacacca gtgcaacggg
     3481 gtgttcaagc acagcacaat cgcgtaacag gctgttgata accgcttcgc tttgcggggt
     3541 ttgtccatct ccgcttgtaa aggctttggc agcagacctg cctaaaactg gatccaacga
     3601 ttggagcgct gcgatcacat acgaaacaaa acgcggaaag agatcatcct catctcccaa
     3661 tgagaaccag gccgctttat gctttttgtt ttgcagccag ttgaccaaca aggtggtctt
     3721 acccgagcca gcaggcgcag agatcagggt caatgctgtg ggggcggccc agccttcatc
     3781 aaggatactg aaaatacgcg gacggacaac cacatccatg cgcggacggg ggatgttgat
     3841 cttggtggga atcatcgcgg ggacggtcat agtggatgaa gggtattata gttaatttgt
     3901 gccattataa aaacatcccg ccgacagatc acgtcggcgg gatgggcggt gacagatatc
     3961 cgttctttta ttggcggttt gaaaccattt gaacaagagt ggggatggtc agcgcggcaa
     4021 agaaagcagc cagcccaaag gaaagcgcag gcagcagcca gcgggcattg acgatgaaat
     4081 cctgataaag aaggatccac tgaatttgca gttttaccac tgccacaggc gggagacttt
     4141 gcaaggcaaa caatacggcg ccaatggata aggcaatgaa caaactcaat acgaaatcat
     4201 tccacgtaac aagcgcgggg cgttgttgag atgggacagc ctgaatgcgt gacatcacat
     4261 tggctgtgat gctgcgcggc atgggcgcaa gcggctggga cttaagcgca tcttcgatga
     4321 gcgcatcttg ttctaggcgg ggatccatga ttcctccagt tgggcaagcg atttgcggag
     4381 ttgttccttg gcgcggaaaa tgcctgtttt tacggtacca agcggcaggt taagagtcga
     4441 ggcgatctct tcgtaggaaa gttcattttg gtatcgaaga gtaatcagca attggtaatt
     4501 tgagtccaac gattgaatgg catcctgcaa atgctttcga gtttcgctga cttcaaagat
     4561 ttgagcgggg gtatccagat acggttcggg gatgtcttcc atcatgtcat cgccgaggtc
     4621 gttgagggag cggcgcaggt tcggcaggcg gttgtagcac aggttggtga cgatgcgata
     4681 caaccacgtg cggaattggc tctgcccctt aaagttaggc agcgccgtcc aggcgcgcac
     4741 gaaagtctcc tgtgccagat cgagcgcttc gttctcatct ttgacgacgc gcaaggcaag
     4801 gttgtagacg tattgttggt gctcattcac cagcgcttcg aaggcttgaa cgtcgccttt
     4861 ttgggctttt tgaacgagga tctgttcaga gattaccatt ttctactggg ttagacaggg
     4921 aaaggggcgg aaaagtttca
//
LOCUS       DAOQJC010000206         4854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_10313_length_4854_cov_7.973953, whole genome shotgun sequence.
ACCESSION   DAOQJC010000206 DAOQJC010000000
VERSION     DAOQJC010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10313_length_4854_cov_7.973953"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..631
                     /locus_tag="PLE39_18610"
     CDS             302..631
                     /locus_tag="PLE39_18610"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNE70403.1"
                     /translation="MMIVQATVIEVEKNNVPEDALLPLEKNGHEVAVCKVGQAYYAFH
                     NNCTHEEWKLSDSYMVDGHIVCSLHGAMFDPTNGVCKRGPACDPLRMYKVVEQDNKLF
                     IDLENEQ"
     gene            628..1887
                     /locus_tag="PLE39_18615"
     CDS             628..1887
                     /locus_tag="PLE39_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70404.1"
                     /translation="MSHKLHDVFPTLKVYVPAVLPGKRLKDSIVGLDTLDKETLLQVS
                     RVAGDAGQLLGHFGANVVTLVELPALFAPLQRSLSDLLESVQADYQALTVRGTEALSE
                     ETVRWQLPEGTPELHHGYNVCDHYFRFVRVKDMKAREWLGTLAFVSLAVVEDLPHTLL
                     NWDEEIALLASLTEMFSWILPEGMEAESSLQSGKPPISSETSLPLWQSEAKNVGRTIS
                     IAYYRLLIGHHIWQHINIFARECFEHSADEFAQGNDEEGTRWLWKATRLFRGTTASMW
                     YASIFPLQTYQAELRPTMVETDSIDAQQQHLTYNLLKQGIKQFKLTMEERAASNKPLH
                     SEQTYTVLKQFHEYYVQDMEQHILVASSKVGLDASLAQKVWQSKLPANTRTKNAMDLL
                     RDMAGIRRKDWQKVLSIAVAQPEMEGT"
     gene            1887..2552
                     /locus_tag="PLE39_18620"
     CDS             1887..2552
                     /locus_tag="PLE39_18620"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE70405.1"
                     /translation="MERILEPELMEDMEQVLAYARADFAASHQLRVTWFHERLGTSVT
                     PTSILDLCCGSGDMTFRFARAFSQSHILGVDGSQAMIEIARQDVQAEPDLAARIEFKQ
                     AFLPDESLPRKGYDMVMSHSALHHFHDPRVLWDAIRMHSPSGSIVFVSDLRRVGSVAE
                     AKQIVNERAGNEHPLLQHDFAASLCAAFTPQEVQSQLDAAGLSQLNVEAIGDVYLLVY
                     GRV"
     gene            complement(2640..3347)
                     /locus_tag="PLE39_18625"
     CDS             complement(2640..3347)
                     /locus_tag="PLE39_18625"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70406.1"
                     /translation="MLERLDLTQALEREEYNKQLKALQAEVHELAFQVYAQKRPVVIV
                     YEGMDASGKGGTIKRLTERIDPRGYVVWPIAAPSGEDKERHYLYRFWRRLPEQGQIAI
                     FDRSWYGRVLVERLEGYCTEADWRRAYGEINQFERQLTDYGTIVFKFWMHISKQEQLR
                     RFRERTRTNYKAWKITDEDWRNRGKWEDYIIAAEEMLLKTSTPDSPWTLVEANDKYFA
                     RIKSLRTVAEKLRRALK"
     gene            complement(3340..4119)
                     /locus_tag="PLE39_18630"
     CDS             complement(3340..4119)
                     /locus_tag="PLE39_18630"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70407.1"
                     /translation="MLEYVNLDLAITQEEYKKQLKPLQQRLYELEHAVFDAKIPVIIV
                     FEGWAATGKGRLISLLAERLDPRGFRVVPITPPRTAETRYPWMWRFWQKIPARGQVVA
                     FDTSWYRRVLIDRLNKTINKGDLRAAYQDIAEFEEMLSADGTVILKFWLHLSEADQKK
                     RIKKLRKDKLTAWQVSEEDLLQNKRYEKYTAFVEDMIARTDSPHAPWTIVEATDRYYM
                     RIKVFETIIASLEARLGLPSSTGAKSKRPRKTKEMKGAVNA"
     gene            complement(4154..>4854)
                     /locus_tag="PLE39_18635"
     CDS             complement(4154..>4854)
                     /locus_tag="PLE39_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70408.1"
                     /translation="TLPLNGQNTGEISDRFQVYFVPAGYVFAIWGIIYIGWIAFSIYQ
                     FRADQKESPRLRRLGYVFAVSGIFNAAWLFCWHYNLFGLSVLVMLTLLGLLIASYLKL
                     NVGRTPVSAAEKWSVDIPFSVYLGWISVATVANVTDYLYFINWAGFGLAPQAWAVVML
                     VVACLLGLLMTVTRRDSGYVFVLVWSFAGIAQKQSAAALVANSAWVATLFMLGLAIYS
                     MVERNRAGNGREVK"
BASE COUNT         1096 a         1197 c         1347 g         1214 t
ORIGIN      
        1 aactgcacca gcatggacat tgcctctgtg gctgtcgctc cctgcgccga taaaatccac
       61 tcaggcagga acagggcgaa gatccccgcc accccaagga caagggcgct ggatgtcatt
      121 aatatttttg tgttcataaa gttctcctgc agtgaagtat aaccaaccgg tggtacgaat
      181 gaccaagact tttggatgag cggaattccc cacctttggg ggagtgctgt aactgttgag
      241 tgaaacacac atgttatata ctcgcgtgag ttgcttctga aaaatcttta ttgcgaggtt
      301 catgatgatc gttcaagcta cagtgatcga agttgaaaag aacaacgttc cagaggatgc
      361 cctccttcca ctggagaaga atggacatga ggtggctgtc tgcaaggtgg gtcaggctta
      421 ttatgccttt cacaacaact gcacacatga agaatggaaa ctgagcgaca gctacatggt
      481 ggatggtcac atcgtttgct ctttgcatgg agccatgttt gacccgacga acggggtctg
      541 caaacgtggc ccagcctgtg acccattgcg gatgtacaaa gtagtggaac aagacaacaa
      601 attatttatt gacctggaga atgaacaatg agccataaat tacatgatgt attccccacc
      661 ttgaaagtct atgtgccagc agtgttgcct ggcaaacgtc taaaagattc gattgttggg
      721 cttgataccc tcgacaaaga aactctttta caggtgtcca gagtggcagg cgatgccggc
      781 caattgcttg ggcattttgg cgccaatgta gtgacattgg tcgaattgcc tgctttgttt
      841 gcacccctgc agagatctct ttctgacttg cttgagtctg tgcaggctga ttatcaggct
      901 ttgaccgttc gcggtaccga agcattgagc gaggaaactg tccgctggca attacctgag
      961 ggaacgcctg aattgcatca cggctacaat gtctgcgatc attattttcg ctttgtgcgt
     1021 gtgaaggata tgaaggcgcg tgaatggctg ggtaccctgg cttttgtatc gttggctgta
     1081 gtggaggacc ttccacatac tttattgaat tgggatgagg aaattgccct gctggcttca
     1141 ttaaccgaaa tgttttcgtg gattctgcct gagggaatgg aagcagagtc aagcttgcag
     1201 agtggcaaac cgccaatttc gtcagagacc agccttccgt tatggcagag cgaggcgaaa
     1261 aacgtcggcc gcaccatctc catcgcgtat tatcgtttgc tcatcgggca ccacatctgg
     1321 cagcatatca atatctttgc gcgtgagtgt ttcgaacata gcgccgatga atttgcgcaa
     1381 ggcaacgatg aagagggtac acgctggtta tggaaggcta cccgcctatt ccgcggcacc
     1441 acggcaagta tgtggtatgc aagtattttt ccattgcaga cctatcaggc tgaactgcgc
     1501 cccaccatgg tggagacaga cagcattgat gcccaacagc aacacttgac ctataatctg
     1561 ttgaagcagg gaattaaaca attcaagctc acaatggaag agcgcgctgc ttcgaacaaa
     1621 cctttgcatt cagagcagac ttatacggtt ctcaagcagt ttcacgaata ttatgtgcag
     1681 gatatggaac aacatatttt ggttgcgtct tccaaggttg ggcttgatgc ttcgctggcg
     1741 caaaaggtgt ggcaaagcaa attgcctgcc aatacccgca ccaaaaacgc catggatctc
     1801 ctgcgggata tggcgggtat ccgccgtaaa gattggcaaa aagttttaag catcgccgtg
     1861 gcacagcctg agatggaagg tacatgatgg aacgcattct cgagcctgaa ctcatggaag
     1921 acatggaaca ggtgctggcg tatgcgcgtg cagattttgc cgcatctcac caattgcggg
     1981 tgacatggtt tcatgaacgc cttggcacga gtgtaacccc cacttccatt ttggacctgt
     2041 gctgcggctc tggcgatatg acctttcgat tcgcccgtgc attttcgcag agccacatcc
     2101 tcggcgtgga tggctcacag gcaatgatcg agattgcccg acaggatgtg caggcagagc
     2161 cagatctggc agcgcgtatt gagttcaaac aggcctttct acccgatgaa tctcttcccc
     2221 ggaaggggta tgacatggtt atgtctcaca gtgccttgca ccattttcac gacccaaggg
     2281 tgctgtggga tgcgattcgg atgcattccc cttctggaag cattgttttt gtttcagacc
     2341 tgcgccgcgt tggcagtgtg gcagaggcaa agcagattgt caatgaacgg gctggcaatg
     2401 aacatccgtt gttgcagcat gacttcgccg catcgctgtg tgcggcattt accccgcaag
     2461 aggtgcagag tcaacttgat gcggctggat taagtcaatt gaatgtggaa gccattggag
     2521 atgtttacct gctggtatat ggcagggtgt agattttgct ttctgcaaac gaattgtaca
     2581 taaatattcc cgccgccgaa gccttcggcg gcgggaatgc ttcaaaagca ggggctggtt
     2641 tacttcaacg cccgccgcaa tttttccgcc actgtgcgca gggatttgat gcgggcaaag
     2701 tatttgtcgt tggcttccac caatgtccag ggggagtcgg gtgtgctggt cttcaagagc
     2761 atctcttctg ccgcgatgat gtaatcttcc catttgccgc ggttgcgcca atcttcatct
     2821 gtgatcttcc aggctttgta attcgtgcgt gtgcgttcgc ggaagcggcg caattgttcc
     2881 tgcttgctga tgtgcatcca gaatttgaag acgatggtgc cgtaatcggt aagttgacgc
     2941 tcgaattggt tgatctcgcc ataggcgcgg cgccaatcgg cttctgtgca atagccttca
     3001 aggcgttcca ccagtacacg cccataccag gaacggtcga agatggcgat ctggccttgt
     3061 tcgggcaatc gccgccagaa acgatacagg taatgccgtt ctttgtcttc gccagagggg
     3121 gcggcgatgg gccagaccac gtatccgcgt ggatcgatgc gctcggtcag gcgtttgatc
     3181 gtgccgcctt tgcctgaagc atccatgcct tcatagacga tcaccacagg gcgtttctgg
     3241 gcatagacct gaaacgccag ctcatgtact tctgcctgca atgccttgag ttgtttgttg
     3301 tattcctcgc gttcaagggc ttgggtaaga tcgagtcgct caagcattga cggctccttt
     3361 catttccttt gttttgcgcg gacgtttgga ctttgcccct gttgaggagg gcagtcccag
     3421 acgggcttcg agtgaggcga tgatggtctc gaagactttg atacgcatgt aatatcggtc
     3481 ggtggcttcg acaattgtcc agggggcgtg aggcgaatcg gtccgcgcga tcatatcttc
     3541 gacaaaggct gtatattttt cgtagcgttt gttttgcagc aggtcttctt cgctgacctg
     3601 ccaggcggtc aatttgtctt tgcgtaattt cttgatgcgt ttcttctggt ctgcttcgct
     3661 gaggtgcagc cagaacttca atatgaccgt gccgtcggcg cttaacattt cttcaaattc
     3721 ggcgatgtct tgataggcgg cgcgcaggtc acctttgttg atggttttat tcaaacggtc
     3781 gatcaagacg cggcgatacc aggatgtgtc aaatgcaacc acctgtccgc gggcggggat
     3841 cttttgccag aagcgccaca tccacgggta gcgcgtttcg gcggtgcgcg gcggtgtgat
     3901 ggggacaacg cgaaagccgc gcgggtcgag ccgttctgcc agcaggctga tcaaccgtcc
     3961 cttgcccgtg gcggcccaac cctcgaacac aatgatgact gggatctttg cgtcgaagac
     4021 ggcatgctcc agttcataaa ggcgctgttg taacggcttt aactgttttt tgtattcttc
     4081 ctgcgtaatg gccagatcaa ggttgacgta ttcgagcatg gcgggctcca gtttgttggg
     4141 tttgacttgg ttgttatttt acctctctcc cgttccctgc cctgtttcgt tccaccatgc
     4201 tgtagatcgc cagcccaagc atgaacaggg tggcaaccca ggcgctgttc gccaccagtg
     4261 ccgcagcgga ctgtttttga gcgatcccag cgaaggacca aaccaacacg aagacgtagc
     4321 cactgtccct gcgggtgacc gtcatgagca ggccaagcag acacgcaaca acgagcatga
     4381 caaccgccca agcctgcggc gcgagtccaa atccagccca gttgatgaag taaaggtagt
     4441 cggtgacgtt ggccactgtg gcgacggaga tccagccgag gtagacgctg aagggaatgt
     4501 ccacgctcca tttttcagcg gcgctgacgg gtgtgcgccc gacgttgagt ttgagataac
     4561 tggcgatgag cagaccgagc agggtgagca tgaccagcac gctcaagccg aacaggttgt
     4621 aatgccagca gaaaagccag gcggcgttga agatgccgct gacagcaaat acatacccga
     4681 gcctgcgcag gcgcggactt tccttttggt cggctcgaaa ttgatagatg ctgaaggcga
     4741 tccagccaat gtagatgatg ccccaaatgg cgaagacata ccctgcaggg acgaaataaa
     4801 cttgaaagcg gtctgagatc tcgcctgtgt tctgcccatt gagcggcagg gtgc
//
LOCUS       DAOQJC010000207         4655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_10960_length_4655_cov_11.180217, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000207 DAOQJC010000000
VERSION     DAOQJC010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10960_length_4655_cov_11.180217"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1297
                     /locus_tag="PLE39_18640"
     CDS             26..1297
                     /locus_tag="PLE39_18640"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNE70409.1"
                     /translation="MPNTSHPLTRNAQTALNNAKRIAEQNGQSSVDSLALLLALLQLP
                     RSQVAAVLMVLKVRVENLIARVSATIKLEAGQAATGSGEKRGGLGLSAETESIMSESI
                     VEMKEQGLDAIDTTTLLLGMLRSPESQAGQILAQYGITAEQVRAGMKSIKEMPIDRVP
                     ASELFKTLGRAMRNGISPIFISLILFTISMAGFLWFRIGSNPQVFMFAFIIGGWLVSL
                     CLHEFGHAITAFWGGDESVENKGYLTLNPLKYTHPIISVVIPLAMLLMGGLAFPGGAV
                     YINIHALRKPGYRSLVSAAGPLANLLCLFLLALPFANIPFYNFFLQAPGEFLSALGLL
                     ALLQMIALFINLLPIPGLDGFGILEPFLPREWLGFASFLRPFGFLIVFFLLSTDSPIS
                     DFFWSNVWSAMELLSFNLAYFANEGVKTFFL"
     gene            1356..1550
                     /locus_tag="PLE39_18645"
     CDS             1356..1550
                     /locus_tag="PLE39_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70410.1"
                     /translation="MNEKAQVNVTDGAQCKVVSGVHAGKAGTIRDIKTGKTGHISITV
                     VQADGERFKTLAKNVVVVKA"
     gene            complement(1660..2616)
                     /locus_tag="PLE39_18650"
     CDS             complement(1660..2616)
                     /locus_tag="PLE39_18650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70411.1"
                     /translation="MKNVKWIAVFFAILSVLILVGIAWSGYGLLRVPEKHLGNPDILL
                     PILVSVSSIGLLVVLAFLAVSFSVLDLADRSQALGLPEGSVRALISLLLIMMFVITSI
                     FLYRQIQFNEEGVQIMDYIGISEDMLNDIPNEEIVAVTVKNNGDEQVFDVKRRIEAVN
                     QEASQRFAEQVLTTISTLVVSVSAFYFGSRSVSVARGTVSSASSPTIRSINLTEGRQG
                     DAAKDVEILGKNFDSPKTVKLVRDSDEIPIEEISASETRIRGKFAIPAGINAGKYDLV
                     VVNKDGGEDRLVNAFEVKKKEPPAQEPVEVTSPTQDPAAAQG"
     gene            complement(2689..3051)
                     /locus_tag="PLE39_18655"
     CDS             complement(2689..3051)
                     /locus_tag="PLE39_18655"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE70412.1"
                     /translation="MKIKATNTILYCKKWRETVDFYRDGLKLLPLSSTDWFVEFKLNE
                     SARLSVADETRTSIKSGEGHGLTISLQVADVDELRAELIQSGFAPTPIKEVWGSQAMY
                     LRDPEGNRLEFWSGRAKA"
     gene            3100..3762
                     /locus_tag="PLE39_18660"
     CDS             3100..3762
                     /locus_tag="PLE39_18660"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HNE70413.1"
                     /translation="MKSSPHPRLAILNLLCIAALLTFGFAPFKDGDETYWDRQLRLRG
                     KMNLPVKTLSQAKYTSCGEAVITMAYNYAYPETQITEREVIEFALAEGYYTEDQAPFT
                     SPDDMVKIAGQYASLVSSGTVTNAEEALALLTDKLTSGDPVIIDTLSRLNDPNSGAHF
                     VIVTGLTIDKNPNRTKIHFNDPLTGTNRSAYWLGIEGVWHGWQNNGDPGGAGWWMVLS
                     SP"
     gene            3795..4598
                     /locus_tag="PLE39_18665"
     CDS             3795..4598
                     /locus_tag="PLE39_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNE70414.1"
                     /translation="MKKFLVKPESKINLEKIDPNDKGNFKGGKEEGLAKLEKLNSKLE
                     ALQELLFAEHKHKVLIVLQAMDAGGKDGTIRRVFDSVNPAGVRVAAFKAPTAEELDHD
                     FLWRIHKQVPGKGEMVIFNRSHYEDVLVVRVHNIVPPEVWKKRFDQINEFERTLAENG
                     TTILKFYLHIDMEEQKERLQARLDDPTKRWKFRLGDLEERKLWPQYMEAFEDVLSKTS
                     TEYAPWYIIPANRKWFRDLAISTILVDTLEDLKMKYPESEEKLDGVVIE"
BASE COUNT         1232 a         1233 c         1112 g         1078 t
ORIGIN      
        1 atttctccac gggtataatc actccatgcc caacacatct cacccactga cacggaacgc
       61 acagactgcg ctcaataacg caaagcgcat tgccgaacaa aatggacagt catcggttga
      121 ctcgctggcg ttgctgctgg cgctgttgca gctccccagg tcacaagtgg ctgcggtgtt
      181 gatggttctc aaagtgagag tggaaaatct tattgcgcgt gtgtccgcca cgatcaaact
      241 cgaagcaggg caggcagcta cagggtcggg agagaagcgc ggagggttgg ggctatctgc
      301 tgaaaccgaa tccatcatga gcgaatccat cgtcgaaatg aaggaacagg gacttgatgc
      361 cattgatacc accaccctgt tgctgggtat gttgcgcagc cctgagagtc aggctgggca
      421 aatactggct caatatggga tcaccgcgga gcaggtccgc gctggcatga agagcatcaa
      481 agagatgccc attgaccgag tccctgcctc tgaattgttc aaaacgctgg ggcgagccat
      541 gcgcaacggc atcagcccga tcttcatcag cctgattttg ttcaccatca gcatggcggg
      601 gttcctgtgg tttcgtattg gaagcaaccc gcaggtcttc atgttcgctt ttatcatcgg
      661 cggctggctt gtttcgcttt gcctgcacga gttcggacat gccatcacag cattttgggg
      721 cggagatgaa agcgtggaaa acaagggcta ccttaccctg aatccgctca aatacacaca
      781 cccgatcatc agtgtggtca tccctctggc aatgctgttg atgggcggac ttgcatttcc
      841 aggcggggcg gtgtacatca atatccatgc gttgagaaaa ccaggctatc gcagccttgt
      901 ctcggcggca ggtccgctgg cgaacctgct ttgtctcttt ttgcttgcgc tgccgtttgc
      961 caacattccc ttttacaatt tctttttgca agcgccaggc gagttcctct ccgcgcttgg
     1021 gcttctggcc ctgctgcaaa tgatcgccct gttcatcaat ttgctgccga tccccgggct
     1081 ggatggattt gggatccttg aaccttttct tccgcgtgaa tggctgggat tcgccagttt
     1141 cctgcggcct tttggattct taattgtgtt ctttctcctc tcaacagatt cgccgatctc
     1201 agacttcttc tggagcaatg tctggtccgc gatggaattg ctcagtttca acctggctta
     1261 ctttgccaat gaaggtgtca aaacgttctt tctctaatgg ctgtttttga aaatttatcc
     1321 gtacatccaa tcaaaccagg aaaaggagat accccatgaa tgagaaagct caagttaatg
     1381 taacagatgg agcacaatgc aaggttgtga gtggcgtcca tgcagggaag gctggtacga
     1441 tcagggatat caagacaggg aagacgggtc acatatccat tacggttgtg caggccgacg
     1501 gagaaagatt caaaaccctt gcgaagaacg tggttgtggt caaggcataa acacattcaa
     1561 tctctggtgg aagaaaaaga gacagtcacc tttgccccaa aagatgactg tctcttttta
     1621 tgatttaatt tttttacaaa accgagaaat taaagagatt catccctgcg ctgcggctgg
     1681 gtcctgcgtc ggcgaagtca cctccactgg ttcttgtgca gggggttcct tcttcttgac
     1741 ctcgaacgca tttacaaggc gatcttcacc accgtctttg ttgaccacga ccagatcgta
     1801 tttcccagca tttatgccag caggaattgc aaactttccg cggattctgg tttcacttgc
     1861 agaaatctcc tcgattggga tttcgtcaga atcacgaacc agtttgacgg ttttgggcga
     1921 atcgaaattt ttcccgagaa tctccacatc tttggcggca tctccctgcc tgccttcggt
     1981 caggttgata ctgcgaatgg ttggagaaga ggcggaggat acagttccgc gcgccacaga
     2041 tacagatcgt gaaccaaagt agaaagccga aacagaaacc acaagtgtgc tgatcgtggt
     2101 aagcacctgt tcggcaaaac gctgactggc ctcctgattc acagcctcaa ttctgcgttt
     2161 gacatcgaac acctgctcat caccattgtt ttttaccgtc acagcaacaa tttcttcatt
     2221 tggaatgtcg ttcagcatat cttccgaaat gccgatgtaa tccataatct ggacaccttc
     2281 ttcattgaac tgaatctggc gatagagaaa aatagatgtg atcacaaaca tcataatgag
     2341 cagcagggag atcaacgctc gcacactgcc ttctggaagc cccagggctt gactgcgatc
     2401 agccagatcc aaaaccgaaa atgagaccgc caaaaaagca agaaccacca gcaggccaat
     2461 ggaactgaca gataccaaaa ttggaagcaa gatatcgggg ttacccaaat gcttttcagg
     2521 cacacgcaga agcccatacc cactccatgc aattcccacc aaaatcaaaa cggacagaat
     2581 ggcaaaaaac acagcaatcc attttacgtt tttcatctct tcctccttcc aatttcatca
     2641 tacagcgatt tgaaaaaaaa tcaattggca ggtgaaagag aacatctttc atgcttttgc
     2701 gcggcccgac cagaattcga gcctgtttcc ttcagggtcg cgcaaataca tcgcttgtga
     2761 tccccacact tctttgatcg gtgtaggcgc aaaacccgac tgaatcaact cagcgcgcaa
     2821 ttcgtccaca tcggctactt gcaggctgat ggtcaggcca tgcccctcac cgctcttgat
     2881 ggaagtgcgc gtttcatcgg ccacgctcaa acgcgctgac tcgttcaact tgaattccac
     2941 gaaccagtct gtactggaaa gaggcagcag cttcaaaccg tcacgataaa agtccaccgt
     3001 ctcgcgccat tttttgcaat ataaaatcgt attcgtcgct ttgattttca tggctgtata
     3061 atagtaccca atctgaatat ttacaactgc aaatttccca tgaaaagctc cccccacccc
     3121 agactcgcaa tcctgaacct tctctgcatt gccgccctgc tgacctttgg ttttgccccc
     3181 tttaaagatg gcgacgaaac ctactgggac cgacaactgc gcctgcgcgg gaaaatgaat
     3241 ctccccgtga aaacgctctc acaggcaaaa tacacttcct gcggagaagc ggtcatcaca
     3301 atggcatata actatgccta ccctgagacc cagatcacgg agcgggaagt gatcgagttt
     3361 gcgcttgccg agggatatta cacggaagat caggctccct tcaccagccc cgatgacatg
     3421 gtcaaaatcg ctggccaata tgccagcctt gtctcaagcg gaaccgtcac caatgcagaa
     3481 gaagcgctcg ccctgttgac cgataaactg accagcggag accccgtcat tattgacacc
     3541 ctttcacggc tcaacgaccc gaattcgggc gcgcactttg tcattgtcac cgggctgacg
     3601 atcgacaaaa atcccaatcg aaccaaaatc cacttcaacg acccattgac aggcaccaac
     3661 cgctccgcat attggctggg cattgaaggt gtctggcatg gctggcaaaa caacggtgac
     3721 cccggcggcg caggctggtg gatggtgttg tcatcgccgt gagccgcaag tcacatcaac
     3781 cctggaggca gttcatgaaa aaatttctgg tcaaacctga aagcaagatc aatctcgaga
     3841 agatcgaccc gaacgacaag ggcaatttca aaggcggcaa ggaggaggga ctcgccaaac
     3901 tggaaaaact gaacagcaag ctggaagccc tgcaggaatt gttgtttgcc gagcacaaac
     3961 acaaggtgct gatcgtgtta caggccatgg atgcaggcgg aaaggatgga acgatccgcc
     4021 gtgtgttcga cagcgtcaac cccgcaggcg tgcgagtagc cgcgttcaaa gcacccaccg
     4081 ccgaagaact cgaccatgat ttcctgtggc gcatccacaa gcaggtaccg ggcaaaggcg
     4141 aaatggtcat cttcaaccgc agtcattatg aagatgtatt ggttgtgcgt gtccacaaca
     4201 ttgtcccgcc tgaagtgtgg aaaaaacgct tcgaccagat caatgaattc gaacgcaccc
     4261 tcgccgaaaa cggcaccacc attttgaagt tctatctgca catagacatg gaagaacaaa
     4321 aggaaagatt gcaggcccgt ctcgatgatc ccaccaaacg ctggaagttc cgccttggcg
     4381 atctggaaga gcgcaaactc tggccgcaat atatggaagc ctttgaagat gtgttgagca
     4441 aaaccagcac agaatatgcc ccgtggtata tcatccctgc caaccgcaaa tggttccgcg
     4501 atctggcgat ctccaccatt ttggtggata ccctcgaaga cttgaaaatg aaataccccg
     4561 aatcagaaga aaaactcgat ggcgtcgtga tcgaataaaa aaggagacag ccatgatttt
     4621 cggtaaaaaa caagaaaaaa gcataagaaa catcc
//
LOCUS       DAOQJC010000208         4551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_11333_length_4551_cov_9.136343, whole genome shotgun sequence.
ACCESSION   DAOQJC010000208 DAOQJC010000000
VERSION     DAOQJC010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11333_length_4551_cov_9.136343"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..692
                     /locus_tag="PLE39_18670"
     CDS             48..692
                     /locus_tag="PLE39_18670"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE70415.1"
                     /translation="MEITWYGHSCFRLTERNYATVVTDPYDSKKVGYEPLKLKAEIVT
                     LSHDAPGHNYSDAVKGSTHVLTGPGEFEIGSVFITAVQTDSAGKKKDKVRNTVYVFDY
                     DGITVAHLGDLQQIPTQSEIELLGTVNVALVPVGGGNSLNAAKAAEVVSMLEPNLVIP
                     MHYATPDSKVSLDAINKFIKEMGLSKPEAQPLLKVSRSGLPDETHVVVLDYQKG"
     gene            694..996
                     /locus_tag="PLE39_18675"
     CDS             694..996
                     /locus_tag="PLE39_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70416.1"
                     /translation="MSVKVIMTWDIAAEHDQEYFEFVIGEFVPGVQRLGLQPAEAWAT
                     IYGSYPQIQVGLLASDMNQARRILASPDWIALQERLFGYVKNYSHKVVPARGGFQF"
     gene            1044..3488
                     /locus_tag="PLE39_18680"
     CDS             1044..3488
                     /locus_tag="PLE39_18680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE70417.1"
                     /translation="MKHTFTKYWPWFFFTAAFESLLAILALLWLPSESGLSMARLGLL
                     GVLFAFFCAGFYGGWIARQNPTRFEKLASTPAIIFTALLFLTSGLVLFLLRYLNPERL
                     LPYYERLSPLLWYLLIVSLQSSIFFLLHKNGFHLQDISARRPVYVSALIAFAFLFSVF
                     VFVAITKLGITKDTAYWGEPGVAIIGWQFMIALLAGFGLLVYSALNPSPSVGVTNYIL
                     PLTLYLTAAALWLSVPVDVLQNSFYSPITPPANTPFPYSDAGFYDYLAQSLLLGTGYL
                     NSIPPRPFYVFFLAILHFLFGQDYSAIITAQTLVLAFFPVSLYFLAKKLHTSAAGVTV
                     ALFAIFRELTGLWISSNTRVANSKIFTTDFPTAMALTVLCLVLIWWLERRDFKSTLIA
                     GGFFGLVLLFRTQSLLVLPAVFALAWFAFQRKTKEWVMAGFVFAIAMILTVLPWLTHN
                     YTVIGQFTFDDPRQSAIIYSQYSFTGNLDLSQFDPAKESVGHRIVTFTLENPTYVAGF
                     ITNHILNTEIGGLLALPLIERFDGLLEPVNLYWVSWDGSLTWYNLVLLLVYLAVIAVG
                     FGVSWRRWQWIGLVPLAVNLGYVLANGISRFSSWRYNLPVDWVIYFYFAIGVVEILGG
                     LFLLFGVNPFSNLHAPSLSEQEPRGISFRDLRPQFAFFLLAFMFVGALPWFARGLAQP
                     RYAAPQSTLLETLITRGHDSTALTSFLSQPNAVLLEGQLLYPRMFRRGEGMASMNPWP
                     AYAIRDYSRIGSILLNDTRSDLIFVTKDLLNFQHGADAIVLACQTDEGYFNVRLIDFG
                     ETSFQSAPLMDPCPEN"
     gene            3523..>4551
                     /gene="mutY"
                     /locus_tag="PLE39_18685"
     CDS             3523..>4551
                     /gene="mutY"
                     /locus_tag="PLE39_18685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNE70418.1"
                     /translation="MPRLSSDLLNWYHKHGRILPWRDHPDPYAVWVSEIMLQQTRVET
                     VIPYFEKWMGLFPNIAALAKADEQTVLTAWEGLGYYSRARNLHKAAKIVVEKFGGELP
                     RNLDDLRSLPGIGRYTVGAIASMAFKMDEPTLDGNLRRVFSRLFDVTEPADAPAGEKI
                     LWELAAKHLPKGEAGDYNQALMDLGATICLPKTPRCLLCPLMQRCEARKNGTQELRPV
                     LKPKKQAPHHVHAAAVIIERGKVLLHQRPADGLLGNMWEFPNARIEADPASGLVTAID
                     AEYKLTVQRKDALGVVQHAYTHFKITVHAFRCKALSIPKNSNLKWVSLDALGDFPMGK
                     VDRQISKML"
BASE COUNT          978 a         1261 c         1091 g         1221 t
ORIGIN      
        1 tcggcgagcg tacccatcgg gtataattgc actttggagg ctcttttatg gaaattacct
       61 ggtacggaca ttcctgtttt cggctcacgg aaagaaatta cgcaaccgtg gtcaccgatc
      121 catacgatag caaaaaggtt ggctacgaac cgttaaaact taaagccgag atcgtaacac
      181 tcagccatga cgcgcccggg cacaactaca gcgacgcggt caaaggctcc actcacgtgc
      241 tgaccggtcc cggcgagttc gaaatcggca gtgttttcat caccgccgtt cagaccgaca
      301 gcgcaggcaa aaagaaggat aaggttcgca acacggttta cgtgtttgac tatgatggta
      361 ttaccgttgc tcatctgggt gacctgcagc agatccccac acaaagcgag atcgaactgc
      421 tcggcactgt caacgtggca ctggtacccg ttggaggcgg aaacagcctc aacgccgcca
      481 aagctgcgga agttgtcagc atgcttgaac cgaatctggt catccccatg cattatgcca
      541 cccccgattc aaaggtgagc ctggatgcga tcaataaatt catcaaggag atgggtctga
      601 gcaaacctga agcgcagccc ctgctcaagg tctcccgctc tggcctccct gatgaaaccc
      661 atgttgtagt tttagactac caaaaaggat aatatgtcgg tcaaagttat catgacctgg
      721 gatatcgccg ccgaacacga tcaggagtat tttgaatttg tcatcggcga attcgtgccc
      781 ggtgtgcaac ggcttgggct tcaacctgcc gaggcatggg caaccattta tggttcctat
      841 ccacaaattc aggtgggttt gcttgcttca gatatgaatc aggcgcgccg catccttgcc
      901 tcacctgatt ggatcgccct gcaagaacgg cttttcggat acgtgaaaaa ttattcacac
      961 aaggtcgtac ctgcccgcgg cggctttcaa ttttaatcaa gcgggtcggc taaaccccga
     1021 cccgcatttt tttcctctct cgaatgaagc atacctttac gaaatattgg ccgtggtttt
     1081 tcttcaccgc ggcctttgag tccctgctgg caattctggc acttttatgg ctgccgtccg
     1141 aaagcggctt gtccatggcc cgccttggct tgctcggagt tctttttgca tttttttgcg
     1201 ctggatttta tgggggatgg atcgcccgcc agaacccaac ccgattcgaa aaacttgcca
     1261 gcacccccgc aatcattttc accgcgcttc tttttctaac ctcgggcttg gtcctgtttc
     1321 tactgcgcta tctcaaccct gaaagactcc tcccgtatta cgaacgtctc agtccgctgt
     1381 tgtggtatct gctgatcgtt tccctgcaaa gcagcatttt ctttctcctc cacaaaaatg
     1441 gatttcatct acaggatatt tcggctcgca gacctgttta cgtttctgct cttatcgctt
     1501 ttgcgttcct gttttctgta ttcgtatttg tagccattac caaacttggc atcaccaaag
     1561 acaccgccta ttggggtgaa cctggtgtag ccataattgg ctggcaattt atgatcgccc
     1621 tgcttgcagg gttcggtctc cttgtataca gcgctcttaa tccatctcca tccgtgggtg
     1681 tcacaaacta tatcctccca ttgactcttt acctcactgc cgccgcactc tggctcagtg
     1741 tccccgtaga tgttttacag aacagttttt attcacccat taccccgcct gccaatacgc
     1801 ccttcccgta ttccgatgcg ggtttttatg attaccttgc ccaaagcctc ttgctgggaa
     1861 caggctacct caacagcatt ccaccgcgcc cgttttatgt tttcttcctg gcgatcctgc
     1921 attttctgtt cggccaggat tattctgcca tcatcactgc gcagaccctg gtattggcat
     1981 tttttcctgt cagcctgtac ttccttgcaa agaaactaca cacctccgct gcaggtgtta
     2041 cggtggccct cttcgccatc ttccgagagc tgaccgggct gtggatctca tcgaacaccc
     2101 gtgtggcaaa ttcgaaaata ttcaccaccg atttccccac tgccatggca ttgaccgtac
     2161 tatgccttgt tctcatctgg tggttggagc ggcgtgattt caaatccacg ctgatcgcag
     2221 gcggattctt tggtttggtg cttttgttcc gcacccaatc tttactggtt ttgcctgccg
     2281 tttttgcgct ggcgtggttt gcctttcagc gcaaaaccaa ggagtgggtg atggctgggt
     2341 ttgtctttgc cattgccatg attctgaccg tcctgccgtg gctgacgcac aactacaccg
     2401 tgatcggtca gtttactttt gatgatcccc gtcaatccgc catcatttac agccaatact
     2461 ccttcactgg caaccttgat ctcagccagt tcgaccctgc aaaagaaagt gtcggccacc
     2521 gtattgtcac ctttacattg gaaaacccaa cctatgtagc gggcttcatc accaatcaca
     2581 tccttaatac tgagatcggc ggcctgcttg cgcttccact catcgaaaga tttgacgggt
     2641 tgttggaacc tgtcaacctg tattgggttt catgggatgg ctccctcacc tggtacaacc
     2701 ttgtcctgct tttggtttat ctggccgtca ttgccgtggg gtttggcgtt tcctggcgcc
     2761 gctggcaatg gatcgggctt gtgccgttgg cggtcaattt gggatatgtg cttgcaaatg
     2821 gtatctcacg gttttcaagc tggcgctaca acctgcccgt ggactgggtt atttattttt
     2881 atttcgccat tggtgtggtg gaaattttgg gcggtctttt tcttctattt ggcgtgaatc
     2941 ctttttcgaa tcttcatgcc ccatctcttt cagagcaaga accgcggggt atttccttcc
     3001 gtgacctacg cccgcaattt gcattttttc ttctggcttt tatgtttgtt ggcgcgctcc
     3061 cctggtttgc caggggattg gcccagcccc gttacgctgc cccgcaaagc acactgcttg
     3121 aaaccctcat cacacgcgga catgacagca ctgccctgac cagtttcctt tcccagccca
     3181 atgccgtatt gttggagggg caactgctct acccgcgcat gttccgaaga ggggagggga
     3241 tggcctctat gaatccctgg cctgcgtatg caattcgcga ttattcgcgc atcggctcga
     3301 tcctgctcaa cgacacgcgc agtgatctga tattcgtcac aaaagacctg ctcaattttc
     3361 aacacggtgc ggatgcaatt gtccttgcct gccagacgga tgaaggctat ttcaatgtcc
     3421 gcttgattga ttttggcgaa acttcatttc aatctgcccc gttaatggac ccctgccccg
     3481 aaaactgacg actgctcacc gaagcaacac attcttccca acatgccccg cctctcttct
     3541 gaccttctaa actggtacca caaacacggg cggattctcc cctggcgcga ccaccctgat
     3601 ccatacgcgg tttgggtttc ggagatcatg ctgcaacaaa cccgtgttga gacggtgatt
     3661 ccctattttg aaaaatggat gggattattc cccaacattg ccgcactggc aaaagcggat
     3721 gaacaaaccg tcctcactgc atgggaaggg ttggggtatt acagccgagc cagaaacctg
     3781 cacaaagccg cgaagatcgt cgttgaaaaa tttggcggcg aattaccccg taatttggat
     3841 gacctgcgca gcctgccagg gattgggcgt tataccgtcg gcgcaatcgc atccatggct
     3901 ttcaaaatgg atgaacccac tctggatggg aacctgcggc gtgtgttttc gcgcttgttt
     3961 gatgtgacag agcccgccga tgcacccgct ggcgaaaaaa tattgtggga gttggcagcc
     4021 aaacatttgc caaaaggaga agcaggcgac tacaaccagg ctctcatgga tttgggggca
     4081 accatctgcc tgccgaaaac cccgcgctgc ttgttgtgcc cgctgatgca acgatgtgaa
     4141 gcccgaaaaa acggcacaca ggaattacgc cctgtgctga aaccgaagaa acaggccccg
     4201 catcacgtcc acgctgcggc ggtgatcatc gaacgcggca aggtgttgtt gcaccagcgc
     4261 cccgcagatg ggttgctcgg caatatgtgg gaatttccaa atgcacgcat cgaagctgac
     4321 cctgcttcag ggttggtcac agccattgat gccgaataca agctcacggt ccagagaaaa
     4381 gacgcgttgg gggttgtcca acacgcttat acccatttca aaattacggt ccatgcgttt
     4441 cgatgtaagg cgctttcaat tccaaaaaat tcaaatctga agtgggtcag cctggatgcg
     4501 ttgggtgatt tccccatggg caaggtggat aggcaaattt cgaaaatgct g
//
LOCUS       DAOQJC010000209         4547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_11354_length_4547_cov_9.514470, whole genome shotgun sequence.
ACCESSION   DAOQJC010000209 DAOQJC010000000
VERSION     DAOQJC010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11354_length_4547_cov_9.514470"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLE39_18690"
     CDS             <1..285
                     /locus_tag="PLE39_18690"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE70419.1"
                     /translation="MTKAMSLDHAREGIRVNAVCPGDTFVDRWLETGYFEGSDPVTLE
                     EAIKESSSYIPMGRFGKPEEIANAVLFLASDEASFVTGHLLLVDGGNTAQ"
     gene            405..560
                     /locus_tag="PLE39_18695"
     CDS             405..560
                     /locus_tag="PLE39_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HNE70420.1"
                     /translation="MLFSPKEKGQGLVEYALILVLVAIVVIAALMILGPLIGNVFSKV
                     NSSLSGV"
     gene            973..1629
                     /locus_tag="PLE39_18700"
     CDS             973..1629
                     /locus_tag="PLE39_18700"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNE70421.1"
                     /translation="MIVVSDLMGTLTTGSPFLGLVDWVKHNQSKLSASLYLAAIMPSY
                     LLAKNGLIDWQAWGQKLMVDSLSYIKDADPEKLAHVSEWIVEHDFWKKRREDVVARLI
                     AHREGGAQVYIASSVVEPFIEPFAKRIGAQVSGTPVEIKNGRVQMVGELVASEKKLDQ
                     ILGRLGVKRVDFAYGDTVLDIPLLEHAEHPVAVYPDAKLKSVALERGWEIIGDTPKLG
                     "
     gene            complement(1718..4420)
                     /gene="ppc"
                     /locus_tag="PLE39_18705"
     CDS             complement(1718..4420)
                     /gene="ppc"
                     /locus_tag="PLE39_18705"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNE70422.1"
                     /translation="MEISQIIHLLGNLLGQVISELESPHIFEIEERIRALAKARRNGD
                     QTSETSLRQEVASLETGEARAVAAAFAAYFDLVNRAEENQRVLILRRYEDETFPEPIR
                     ESIGEAVATLKELGITRGQMEDLLQNLSIELVLTAHPTEARRRTVLSKMERITELITQ
                     ISTGSLSLREQEEATQLLRNEISVLWLTDRARSAKLSVADEVKTGLYFVDSFFWNTIP
                     VIYDDLEKALERNYPGLHPPADWFKLASWIGGDRDGNPNVTAEVTAETLRLHRGLAVE
                     NHRKSFQELARHLSVSSHRIPPPQELMEWIEKRRPFPPHIAYIENRYVTEPYRLALSL
                     LASDLAEASREDMKAHLLQSYAYQARLNTDGLSRPLEMIASAIPFKLALNELQDVHRK
                     LMIFGLQAARLDIREDSSRFNAALGEILRALKIEPEFENLPADSKIELLVRLLKETAP
                     QLSKHPGATSATAETWSLFQLIGRTHEIYGSDLLGPIIISMTHAASDVLTVLLLAKWA
                     GCKTTPQITPLFESVPDLKDAPRILESLFTLDIYREQLTAHNDEQMVMIGYSDSNKDG
                     GYLMANWSLYEAQEEITRVASEHGIKLTIFHGRGGTIARGGGPANSAIRAQPAGSING
                     RFRLTEQGEIIALRYSNPGLAHRHLEQIASAVILASAPLPHHPISNHWRNAMAQMSEA
                     AQRTYRQMVYETPGFIEFWQSATPLDQIKHLQIGSRPSARSQTGAVNQIRAIPWVFSW
                     MQSRFNLPGWYGLGSGLAMITDRSLLREMYDGWAFFQTLLTNTEMSLLKADMDISALY
                     VDLVPDKALAKEIFSSIRSEYERTREAVLSISRHSTLLELEPATRQAVQLRNPYVDPL
                     NYIQVETLRRLRTLSNPESDEGNALREVMAITINGIASGLRNTG"
BASE COUNT         1009 a         1010 c         1371 g         1157 t
ORIGIN      
        1 atgaccaagg caatgtctct cgatcatgcc cgtgagggaa tccgtgtgaa tgcagtttgc
       61 cccggcgata cctttgtgga ccgatggctg gagaccggtt actttgaagg ttccgacccg
      121 gtgacattgg aagaagccat caaagaatca agttcataca ttccaatggg gcgttttgga
      181 aagcctgaag agattgccaa tgctgttctt tttctggctt cagatgaggc atcttttgta
      241 acagggcacc tgcttttggt ggatggcggg aatacagcgc aataaatgtt ttgtgcaaaa
      301 tagcccaaat ggggccaata acaacagatt tgtaggttga ttccggataa tttctggcta
      361 taatgaaatt gctgtaccat accaaaggaa aggaggtagt caccatgtta ttctcaccga
      421 aagagaaagg tcaaggtttg gttgagtatg cgctgattct tgttttggtt gccatcgtcg
      481 ttatcgctgc gcttatgatt cttggaccat tgatcggaaa tgtattcagc aaggtcaaca
      541 gcagcttgtc tggtgtgtaa ttgcatcaga taggaagcaa agaagtccgt atttgggcgg
      601 cccgttgcat gcaatgggcc gcccattgtt tttcaccaaa ttactgaact aactgatttg
      661 ttaactaaac tgaccagctt tttaattata ataacagtgc cgtatttgaa tttttaagaa
      721 aggaggtaaa aaaatatgtt attcgcaccg aaagagaagg gtcaaggtct ggtcgaatat
      781 gcgctaattc ttgttttagt agccatcgtt gttattgctg cgcttatgat attgggcccg
      841 atcatcggta atgtatttag taaagtaaat agcagcctgt ctcgtgtgta gtagcagaca
      901 gagaatgtca gaatcatgca tgggcggccc gttgttccca acgagccgcc catgcattgc
      961 gtagaggaaa ccatgatcgt tgtatcagat ttaatgggca cattgaccac agggtcaccg
     1021 tttttggggt tggtggattg ggtaaagcat aaccaaagca agttgagcgc cagtctgtat
     1081 ctggcggcga tcatgccgtc ttatctgctt gcaaaaaatg gattaattga ctggcaggcc
     1141 tggggacaaa agttgatggt ggacagcctg tcttatatca aggatgcaga ccctgaaaaa
     1201 ctggcccatg tatccgagtg gatcgtggaa catgatttct ggaagaagcg ccgcgaggat
     1261 gtggtggcac ggttgatcgc ccatcgagaa ggcggagcac aggtgtatat tgccagcagc
     1321 gtggtggagc cattcatcga accgttcgca aaacggatcg gggcgcaggt cagtggtacg
     1381 cctgtggaaa taaagaatgg ccgtgttcaa atggtgggtg aattggttgc aagcgagaaa
     1441 aaactggatc agattctggg ccgcctcggt gtaaagcgtg tggattttgc ctatggcgat
     1501 accgtgctgg atattcccct gcttgaacat gctgaacacc ccgtggcggt ctaccccgat
     1561 gcgaagttga aatctgttgc gctggagcgg gggtgggaga tcattggtga cacgccaaag
     1621 cttggttaga gttgaaagtt acaggtttaa gagttgaagg tcttgaagaa gttttacatt
     1681 tcttcaagac ctttttattt tctattattc gataggctca ccctgtattt cttaaccctg
     1741 acgcgatccc attaatggtg atcgccatga cttcacgcag ggcattgcct tcgtcgcttt
     1801 cggggttgga gagtgtacgc aggcggcgca gggtttccac ttggatgtag ttgagcgggt
     1861 ccacataggg gttgcgcagc tgcacggcct ggcgggtggc gggttcaagc tccagcagtg
     1921 tggaatgtct actgatggaa agaacagctt cccgcgtacg ttcatattcg ctgcggatcg
     1981 aagagaagat ttccttcgcc agcgctttgt ctggaaccag gtccacgtac aaggcggaga
     2041 tatccatatc cgctttaagc agggacattt cggtgttggt gagcagggtt tggaagaagg
     2101 cccagccatc gtacatttca cgcaagagcg aacgatcggt gatcatggcc agccctgaac
     2161 caagcccata ccagcccggc aggttgaagc ggctttgcat ccacgagaag acccatggaa
     2221 tggcgcggat ctgattgacc gcaccggttt gcgaacgtgc cgatgggcgt gagccgatct
     2281 ggagatgttt gatctggtcg agcggtgtgg ccgattgcca gaattcaatg aagcccgggg
     2341 tttcataaac catttggcgg taggtgcgtt gtgccgcctc tgacatttgt gccatggcgt
     2401 tgcgccagtg atttgaaatg gggtgatgcg gcagcggcgc tgaagcaagg atgacggcgc
     2461 tggcgatctg ttcaagatga cgatgtgcca ggccgggatt ggaatagcgc agggcaatga
     2521 tctcgccctg ttcggtcaag cgaaaccgac cattgatact gcctgccggt tgagcacgga
     2581 tggcgctgtt ggcgggtccg cctccgcgcg caatggttcc gccgcggcca tgaaagatgg
     2641 tgagtttgat gccgtgttcg cttgccacgc gggtgatctc ttcctgtgct tcgtaaagcg
     2701 accagtttgc catgagataa ccgccgtctt tgttgctgtc ggagtagccg atcatcacca
     2761 tttgttcgtc gttgtgggcg gtgagctgtt cgcggtagat atccaaggta aaaagtgatt
     2821 cgagaatgcg cggcgcgtct ttcaggtctg gcacggattc gaagagcggt gtgatctgtg
     2881 gggtcgtttt acagcctgcc cattttgcca acaggagaac ggtgagcaca tctgatgcgg
     2941 cgtgcgtcat tgagatgatg atggggccga gcaggtcact tccgtagatt tcatgagtgc
     3001 gcccgataag ttgaaacagt gaccaggttt cagcggtggc cgaggttgcc ccgggatgtt
     3061 tggaaagctg cggagcagtt tcctttaaca ggcgcaccaa taattcgatc tttgaatcgg
     3121 cgggcaggtt ttcaaattcg ggttcgatct tcagcgcgcg cagaatttcg ccgagtgcgg
     3181 cattgaagcg gcttgagtct tcgcggatgt cgagccgtgc ggcctgcagg ccgaagatca
     3241 ttaatttgcg gtggacgtcc tgcaattcgt tcagggccag tttgaatgga atggccgagg
     3301 caatcatctc caacgggcgc gagagaccat cggtgttgag gcgggcttga tatgcataac
     3361 tttgcaacag gtgtgctttc atgtcttcgc gtgaggcttc ggcgaggtcg gaagccagca
     3421 gagacagggc cagacggtag ggttccgtga cataccgatt ttcgatgtag gcgatgtgag
     3481 gtggaaatgg tctgcgcttt tcgatccact ccattaattc ctgaggcggt ggaatgcggt
     3541 gtgagcttac acttaaatgg cgggccagtt cctgaaaact cttgcggtga ttttcaaccg
     3601 ccaggccgcg atgcaggcgc agggtttcgg cggtcacttc agcggtgacg ttgggattcc
     3661 catcgcggtc gccgccgatc catgaggcca gtttgaacca gtcggcgggc gggtgcaggc
     3721 cggggtagtt tcgttcgagc gccttttcaa ggtcatcgta aatgacgggg atggtgttcc
     3781 agaaaaaaga atcgacaaag tacaacccgg tcttcacttc atcggctacg gaaagtttgg
     3841 cagagcgggc acggtcggta agccataaaa cggatatttc attgcgcagg agttgggtgg
     3901 cttcctcctg ttcgcgcagg gaaagtgagc ctgtgctgat ctgggtgatg agttccgtga
     3961 tgcgttccat tttggagagc acagtacggc ggcgtgcttc ggtggggtgg gcggtgagga
     4021 cgagttcaat ggatagattt tggagcaggt cttccatttg tccgcgggtg atgccgagtt
     4081 ctttgagggt ggcaacggcc tcgccgatgg attcgcggat cggctctgga aaggtttcgt
     4141 cttcgtaacg gcgcaggatc aacacgcgct gattctcttc cgcgcgattg accagatcaa
     4201 agtaggcggc gaacgcagcg gcgaccgccc gcgcttcccc tgtttcgagc gaagcaactt
     4261 cctgcctcag gcttgtttcg gaggtctggt cgccgttgcg gcgggctttt gccaatgcgc
     4321 ggatgcgttc ttcgatttcg aagatgtgcg gcgactcaag ctctgaaatg acctgtccca
     4381 gaagattgcc gagcaggtga atgatttgag agatttccat gggctttcct cttttgaaat
     4441 gcttcgccga ttataacttc cgccactttt aatttgccga atctctgaag cggggagtgg
     4501 ctaatttcgt gggcttgcca accaaagatt taaccacaga gcgcaca
//
LOCUS       DAOQJC010000210         4520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_11451_length_4520_cov_10.370885, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000210 DAOQJC010000000
VERSION     DAOQJC010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11451_length_4520_cov_10.370885"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..724)
                     /locus_tag="PLE39_18710"
     CDS             complement(146..724)
                     /locus_tag="PLE39_18710"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNE70423.1"
                     /translation="MAQFQFVMRSGPTPGVTFPLEGDQLIIGRDASNSITINDAEISR
                     KHSRLSFQGGKYVLEDLGSTNGTFVNGQRLAGPVVLKPGDVVSLGEQIVLMYDAINID
                     TGATIATSRKSARVEPPPAAAVSQPPAPTPTPVYTPAPSYSGPPAVKKTNMLPIFIGG
                     GVLIFVCFCISLLWWIDATYRWCVFFPFLTGC"
     gene            complement(864..2105)
                     /locus_tag="PLE39_18715"
     CDS             complement(864..2105)
                     /locus_tag="PLE39_18715"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE70424.1"
                     /translation="MAEIKTERLDSYRRLIDIARDLASTLDLDILLSRIVHAAAEISG
                     AEAASILLYDDTSRQLYFQVSTNLDESTRRGIVVPLEGSIAGWIVNNRRPVRITNAHE
                     DPRFYSDVEATTGFSTDSLLGIPLVTKNKIVGVLEALNKHRGKFTDSDESMLLVLGAQ
                     AAVAIENARLFQQSDLISEFVHELRTPLSSLSTATYLLLRPEMSQEQRDQIINNIHSE
                     TMRLNALASSFLDLARLESGRVQFRKTTFSVADLIYECKDVMSSKAIEDNIQIRVESP
                     EGLPLLEADREKIKQVLLNLLSNAIKYNRPNGTVMLRAELTEKELVIYVQDTGLGIPD
                     ESLPHLFEKFFRVREHESRIAGTGLGLSICKQIIHGHGGRIEVKSKIGVGTVFSIFMP
                     RTGKGQPLPEDPLDPKKIIKK"
     gene            complement(2098..2874)
                     /locus_tag="PLE39_18720"
     CDS             complement(2098..2874)
                     /locus_tag="PLE39_18720"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNE70425.1"
                     /translation="MTSFSPQTQAAIEFAADLLRQSSHAVVLTGAGLSTPSGIPDFRS
                     TGTGLWAKDEPLEVASLSTFRTAPERFFQWFRPLASQIFNAQPNLAHWALAELEKAGH
                     IDSIITQNIDTLHQKAGSKVVIEMHGTMQTLTCTQCYHQVDAGNYLAAFVETGEIPLC
                     PKCGQVLKPDVILFGEQLPQTAWFKAQRAARQCDLMLVIGSSLEVLPVAGLPMQALDR
                     GAHLVIVNNTQTYLNVRADAVIPEDAAVTIPAIVERVLHG"
     gene            complement(2871..4028)
                     /locus_tag="PLE39_18725"
     CDS             complement(2871..4028)
                     /locus_tag="PLE39_18725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE70426.1"
                     /translation="MAEKILIIDDDVDTLRLVGLMLQRQGYEISAASNGTQGLAKALE
                     ERPNLILLDVMMPDMDGYEVTRRLRKNPVTVSIPILMFTAKTQLDDKVIGFEVGADDY
                     LTKPTHPTELQAHVKVLLARQKDVTEIVTSSKEHVGYVIGVLSARGGLGVSSVAANLA
                     AGIHTRIKADVILAELTPGQGTLGMDLGSPNQKGLTELLEGTTAEVTREKVKSLLVSH
                     NSGLKLMLASENPRDVTLNSKVPNYEAIVAQLSTLARFVILDLGNGLPVHVQKLLPMC
                     TERMVVVEGVPGSIQHTRLLIDEIANLKIDRKTITVVLNNRMRSEAQMQLNQVQELLG
                     HSVSATLTPAPEAFLQATRLQTPVVIGQPSNMTSQQFLKLSDAVLEREKAR"
     gene            4151..>4520
                     /gene="ssrA"
                     /locus_tag="PLE39_18730"
     tmRNA           4151..>4520
                     /gene="ssrA"
                     /locus_tag="PLE39_18730"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1146 a         1190 c         1138 g         1046 t
ORIGIN      
        1 taatggattt tcttggcgtt cttcgcgggc ttggcggttc gaaaggcttg tcagacatta
       61 atttgagaaa gccataaggc aggcggatgg gaaaagaaaa agcccgcgag gctgtacctg
      121 cgcgggcttt gaaaatcttt ttggattaac aacctgtgag gaaggggaag aatacacacc
      181 accgataagt agcatctatc caccaaagca ggctgataca aaaacagaca aagatgagga
      241 cgccgccacc gatgaatatg ggcaacatat tcgtcttttt gacggctggc ggaccggaat
      301 aagaaggtgc aggagtgtag accggagtgg gagtgggagc aggcggttga gatacagcag
      361 ccgctggcgg cggttctaca cgtgcagatt ttcgggaggt ggcaatcgtg gcaccggtgt
      421 caatgttgat cgcgtcatac atcaggacga tctgttcacc gagggaaacc acgtcaccgg
      481 gtttgaggac gaccggtccc gccaaacgct gtccgtttac aaaggtaccg ttcgtagaac
      541 caaggtcttc gagaacatac ttgccgcctt gaaaagagag gcgggaatgt ttgcgtgaaa
      601 tctctgcatc attgatcgta atgctgttgg atgcgtcacg tccaatgatg agttgatcgc
      661 cctccagggg aaaagttacg ccgggcgtcg gtcccgacct cataacaaat tgaaactgag
      721 ccatcatctt cctccaaaga aaaaattact gtcttgctcc gacaatttat caaatttttc
      781 gaaaattgcg gacgaatatc atgattctgt tttggtaaat tatacgatac ttcgctgccg
      841 gcaccataaa ggaggccaat ttatcatttt ttgatgattt tcttggggtc cagggggtct
      901 tcggggagcg gctggccttt tccagtgcgg ggcatgaaga tggaaaaaac ggtccccaca
      961 ccgatcttgc tcttcacctc aatgcggcca ccatgaccgt ggatgatctg cttgcagatg
     1021 gaaagcccca acccggtgcc ggcaatgcgc gactcgtgtt cacgcacacg gaagaatttt
     1081 tcgaacaagt gcggcaagga ctcatccggg ataccaaggc cggtatcctg cacgtaaatg
     1141 accagttctt tttcggtcaa ttcggcgcgg agcatcaccg tcccgttcgg gcggttgtat
     1201 ttgatggcat tgctcaacag gttcaaaagc acctgtttta tcttttcgcg gtctgcttca
     1261 agcagcggca gtccctcagg cgattcaacg cggatctgaa tattatcctc gatcgccttg
     1321 gaagacatca catccttgca ttcgtaaatg agatctgcca cgctgaaggt ggtcttgcgg
     1381 aactgtaccc gtccagattc aagcctggcc aggtcaagga aggaggaagc caatgcattc
     1441 aaacgcatgg tttcgctatg gatattatta atgatctgat cgcgttgctc ctgcgacatc
     1501 tccgggcgca gcaacagata agttgccgta ctcaaagaag agagcggcgt gcgcaattca
     1561 tgtacaaatt cagagatcag gtcagattgc tggaacaagc gtgcattttc aatggcaacc
     1621 gctgcttgag cacccaaaac cagcagcatg gattcatcgg agtcggtgaa tttgccgcgg
     1681 tgtttgttca acgcttccaa cacacccacg atcttgttct tggttaccag aggaatgccc
     1741 aaaagcgaat cagtggaaaa acccgtggtc gcctccacat cggagtaaaa acgggggtct
     1801 tcatgtgcat tggtgatacg aacggggcgg cgattgttca cgatccaccc tgcaatactc
     1861 ccctccaacg gcacgacaat gccgcggcgg gttgattcat caagatttgt tgagacctgg
     1921 aaatataact gtctggaagt atcgtcatac agcaaaatgg aggcggcctc agcaccgctg
     1981 atctcagccg cagcatgtac gattcgagac agcaggatat cgaggtccag ggtggaagcg
     2041 agatcccgcg caatatcaat aagacggcga taactatcca atcgttcggt ttttatttca
     2101 gccatgtagc actctctcca caatcgcagg aatggtcact gcagcatcct ccgggatcac
     2161 cgcatcggcg cgcacgttca aataggtctg tgtattgttg acaatcacaa gatgagcgcc
     2221 gcgatccagg gcttgcattg gcaaaccagc cactggtaaa acttcaaggg atgaaccgat
     2281 caccagcatc aggtcacatt ggcgggcggc ccgctgggcc ttgaaccacg ccgtttgagg
     2341 cagttgttca ccaaacagaa tcacatctgg cttgagcacc tgaccacatt ttgggcacaa
     2401 cggaatttct cccgtctcca caaatgcagc cagataattc cctgcatcca cttgatgata
     2461 acactgggta cacgttaaag tttgcatggt accgtgcatt tcgatcacaa cttttgaacc
     2521 tgctttttga tgcaaggtat caatattttg ggtaatgatg gaatctatgt gtcccgcctt
     2581 ctcaagctcg gccagcgccc aatgcgcaag gtttggctgg gcgttgaaaa tctggctggc
     2641 cagcggtcga aaccattgaa aaaaccgttc cggtgccgta cgaaaggtgc tgagcgaagc
     2701 cacctccaac ggctcatcct ttgcccaaag acccgtccca gtggaacgga aatcaggtat
     2761 tcccgatggg gtggaaagtc ctgcgcccgt cagaacaaca gcatggctgg attggcggag
     2821 caaatccgca gcgaactcaa tcgcagcttg tgtctgaggg gaaaaggatg tcatcgcgcc
     2881 ttttcacgct caaggaccgc atctgataat ttcaaaaatt gctgtgaggt catattcgaa
     2941 ggctgcccga tcacaaccgg ggtttgcagg cgggttgcct gcaaaaatgc ttcaggggca
     3001 ggggtaaggg tggccgaaac ggaatgtccc agcagttcct gcacctgatt caactgcatc
     3061 tgtgcttcgg accgcattcg attattaaga acaaccgtaa tggtctttct gtctatcttc
     3121 aaattggcga tctcatcaat cagcaggcgg gtatgttgaa tggaaccagg caccccttcc
     3181 acaacaacca tacgctcagt acacatgggc agcagtttct gcacatggac aggcagacca
     3241 ttccccaaat ccagaatgac gaaacgcgcc aacgtggaaa gttgagccac gatcgcctca
     3301 taattcggca ccttcgaatt caaggttaca tcccgtggat tttcagaggc aagcatcaac
     3361 ttcaacccgg aattatgcga aacgagcagt gatttcacct tctcgcgtgt cacttccgca
     3421 gtggtcccct ccaacagttc ggtgagtccc ttttgattcg gcgaccccaa atccataccc
     3481 aaagtgccct gccccggcgt aagttccgca agaatcacat ccgctttgat acgggtatga
     3541 attccagccg caaggttggc tgccacagaa gaaaccccca gcccgccacg ggcagaaagc
     3601 acaccaatca catatcctac atgctccttt gaagaagtga cgatctcagt cacatccttc
     3661 tggcgtgcga gcagaacttt tacatgcgcc tgtaattcgg tgggatgtgt cggcttggtc
     3721 aggtaatcat ccgcacccac ttcaaaaccg atcaccttgt catccaactg cgtcttggct
     3781 gtaaacatca gaatcggaat acttacagtt accgggttct ttcgtaaacg ccgcgtaacc
     3841 tcgtatccat ccatatccgg catcatcaca tccagcaaaa tcaggttggg gcgttcttcc
     3901 aatgccttcg caagcccttg tgtgccattc gaagccgcac tgatctcata accctgtcgc
     3961 tgtaacatca gcccgacaag tctgagggta tccacgtcat catcaatgat caatattttt
     4021 tcagccattt ttcgcctctg ttacctgaat acagtctagc atatccaatt ataacggaca
     4081 agtcagcaga ttacacctca atcccatgct ataataaatg tgcatcgggc acatgccccc
     4141 gtgccgattt ggggatgatc ggcttcgacg ggagaggctg gttccggaat gcgagccgag
     4201 agcgcgccga actcgtaaac tcagcgcaac cattaagtgc caatcgcact tcatacgctg
     4261 ctatgcccct cgccgcctaa ggtgagacat aacagtcaat ctcctagatg agattgcgtc
     4321 caccgtcacc gctccctgcc gggtgacgga ccgggcgaaa caaaggcggg gtgctgcgta
     4381 tgactctgct aaatacgccc aagaccagca gatcgatcac agggttacct gcccgctgag
     4441 atctctgtga tgacaaaaat cggtgggcta cgctcgtaga gttccgaggg tcgaatcttt
     4501 cggacccggg ttcaattccc
//
LOCUS       DAOQJC010000211         4367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_12066_length_4367_cov_8.886364, whole genome shotgun sequence.
ACCESSION   DAOQJC010000211 DAOQJC010000000
VERSION     DAOQJC010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12066_length_4367_cov_8.886364"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="PLE39_18735"
     CDS             complement(<1..1316)
                     /locus_tag="PLE39_18735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE70427.1"
                     /translation="MSNETLSQLQVELKEAQQKIADLEAQLRAVNPGKIPVSKNNGGF
                     VSGGQANDGARREKEKVNALFLNAPLPKNVEDASQESEAKYRLLFEMMTQGVVFQDEA
                     GRILHANPAAEKILGLTVEQMQGRSHVNKDWPSIHEDGSALSAEEHPVAIVMKTKQPV
                     HNMIMGVFNPLRKSYIWLNINSVPRFLEGGTSPYQIFTTFEDITERKKIEFALRESEA
                     KYCSLIDSQESAISTIDADGVIHYMNSRGAAPFGTPETVAGKKLYDLFPLQVAEWQMS
                     QIRQVIESGKGLVHEYQNLVAGRPNWRRVSIQPIINADAGVRLATVNSLDITERKRAE
                     EELRNSERKYRDLLNGMNDTVWVIDDDLSILDVNTAAVSTLGYTREELLSMRVPDFDG
                     SLSPDAIQNLVNTMPADKFQVFQTEHTTKDGRKLPVEISSSLVTYNG"
     gene            1584..2783
                     /locus_tag="PLE39_18740"
     CDS             1584..2783
                     /locus_tag="PLE39_18740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE70428.1"
                     /translation="MNTQYKLYPYRWVVLAAFAFINLTIQTLWIAYAPITGPAAKFYG
                     VTDLQIGYFAMSFMIAFVPLSIPVSWLIDTYGFRIAVSIGAILMGIFGIVRGFAGANY
                     TLALWSTFGLAAAQPFLLNAWTKVPANWFAMEERATAVGIVTLGNLVGTALGMVLTPI
                     LLESMTIPNIQLMYGGVAAFSAVLFVLLARETPPTPPCAPGQEVRALMLDGLKYSFTV
                     KAFWFTLLVSFIGLGIFNGVTTWIEGIIRPRGFSPTDAGTLGALMIVGGVIGAVLMPA
                     LSDKQRKRQLFLYIAFLGSIPGMAGLTYATSSTLLYASAFVLGFFLTSAMPIAMQYAA
                     EVTHPTPEGTSNGLIQLFGQGAVVFVYIMEAMKSADGSFTPALLLAMGLLLASLFFVS
                     QMKDKAQ"
     gene            3075..3314
                     /locus_tag="PLE39_18745"
     CDS             3075..3314
                     /locus_tag="PLE39_18745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70429.1"
                     /translation="MKKTPGQKSNNIQEMSAEYRFDYKKAKPNRFAEKMKSEPVIVIL
                     DADVAKVFTTAVEVNTALRALISAMPPKKKKAAVR"
     gene            3377..4321
                     /locus_tag="PLE39_18750"
     CDS             3377..4321
                     /locus_tag="PLE39_18750"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNE70430.1"
                     /translation="MPPTLNDIQQAAERIKPYAHRTPVLTNESLNQKVGAQVYLKCEN
                     LQKVGAFKFRGACNAVYSLTDEEASHGVVTHSSGNHAQALALAAKMRGIPAYIVMPSN
                     APQVKKDAVAGYGGQITFCEPTLAARESTMEGIRQKTGATVVHPYDNEKVIAGQGTAA
                     MELLEDVPDLDVIITPVGGGGLLSGTSIAAMGLKKGIRVIAGEPEKADDAFRSLQADR
                     IIPSENPKTIADGLLTSLGALTFPIIQQNVEQIVTVSEEGIITTMKFVWERAKIIIEP
                     SSAVAIGVLWENKINLHGLKIGVIISGGNVDLNKLPWQ"
BASE COUNT         1102 a         1192 c          996 g         1077 t
ORIGIN      
        1 ccgttatatg tcaccagact ggaactgatc tctacaggga gttttcggcc atcttttgtt
       61 gtgtgctctg tctgaaatac ctgaaactta tccgcaggca tggtattgac aaggttctgt
      121 atggcatcag gagaaaggga gccatcaaaa tcaggaacac gcatggagag aagttcttca
      181 cgggtatacc caagcgtgga taccgccgcg gtattcacat ccaggatcga caggtcatcg
      241 tcaataaccc acaccgtgtc gttcattcca ttgagcaggt cacgatattt tctttcgcta
      301 tttcgtaatt cctcctccgc gcgctttcgc tcggtaatat ccaaagaatt gacggttgca
      361 agccttacgc cagcatctgc attaatgatc ggctgaatgc tcacgcgccg ccaattcggc
      421 ctgcctgcaa ccaaattctg atattcatgc accagccctt ttccagattc gattacctga
      481 cggatttgac tcatctgcca ctctgctacc tgaagcggga atagatcgta caattttttt
      541 cctgccacgg tttcaggtgt accgaacggg gctgcaccac gagaattcat gtaatgaata
      601 acaccgtctg catctattgt tgaaatggca gactcctggc tgtcaatcaa gctgcagtat
      661 tttgcctcgc tctcccgcag ggcgaattca atcttttttc tttcggtaat atcttcaaat
      721 gtggtgaaaa tttgatacgg actggtcccg ccctcaagaa aacgcggtac cgagtttatg
      781 ttcaaccaga tataggattt tctcaatgga ttgaacacgc ccatgatcat gttgtgaaca
      841 ggctgtttgg ttttcatgac aatcgccacg gggtgttcct ccgccgaaag agcagagcca
      901 tcttcgtgta tgcttggcca atctttatta acgtgggaac gtccctgcat ttgttcaacg
      961 gtcagcccca atattttttc agcagccggg ttggcatgaa ggattcggcc agcttcatcc
     1021 tgaaaaacaa ccccttgggt catcatttca aaaagcaatc tgtattttgc ttcgctttcc
     1081 tggctggcat cttccacgtt ctttggaagc ggcgcattaa gaaaaagcgc attgaccttt
     1141 tccttttccc tgcgggcccc atcgttggcc tgtcccccac tgacaaaccc accattattt
     1201 tttgacacag gtatcttgcc agggtttaca gcccgcaatt gcgcttcaag gtcggcaatt
     1261 ttctgttgcg cctctttcaa ttccacctgc aattgagaca aggtttcatt gctcataatt
     1321 ttttccttat tatagaaaaa agcacccaag aaacatgctt caagaaggta taaataaggc
     1381 aaaaataata tactatacag gctgtgtcca tccgtaatat tacttaataa cgcttgcttt
     1441 tcaactctga aacgcataac aaatctgtta taagcgacca tgtttatcga tcaagatttt
     1501 gacagttttt cataaaaggt gaaaacacac aaggcaaaat ttaccgtacc tgatatgatt
     1561 ggccccagcc aaaaggaaac aatatgaaca cacagtacaa actctaccct tatcgctggg
     1621 tggtgctggc agcttttgcc ttcatcaacc ttaccattca aaccctgtgg atcgcctacg
     1681 cccccatcac aggtcccgca gccaaattct acggtgtcac cgatctgcag atcggctact
     1741 tcgccatgtc cttcatgatc gcctttgtgc cgctctccat ccctgtttcg tggctcattg
     1801 atacctacgg cttccgcatc gcggtcagca ttggcgcgat ccttatgggt atcttcggca
     1861 tcgtgcgtgg attcgctggc gcgaattaca ccctcgccct gtggagcacc ttcggtctcg
     1921 ccgccgccca gcccttcctg ctcaacgcgt ggacgaaagt ccctgccaat tggtttgcaa
     1981 tggaagaacg cgccaccgct gttggcatcg tcaccttggg caatctcgtc ggcacagccc
     2041 tcggcatggt attgacaccc atcctgcttg aaagcatgac catcccaaac atccagctca
     2101 tgtacggtgg agttgcggca ttctcggcag tactcttcgt cctgctggca cgcgagaccc
     2161 cgcccacccc gccctgcgcg ccgggccagg aagtccgcgc cctgatgctg gatggtttga
     2221 agtactcatt cacggtcaaa gctttttggt tcaccctgct cgtctccttt atcgggctgg
     2281 gaatcttcaa cggtgtcacc acatggatcg aaggcatcat ccgtccgcgt ggattttctc
     2341 ccacagacgc gggcacactc ggcgcattga tgatcgtcgg cggcgtgatc ggagccgtgt
     2401 tgatgcctgc tttatcggat aagcaacgga agcggcaact cttcctctac atcgccttcc
     2461 tcggttccat cccagggatg gctggtctga cctacgcgac atcgtccacg ctgttatacg
     2521 cctctgcctt cgttttggga ttcttcctca ccagcgccat gcccattgcc atgcaatacg
     2581 ccgcagaagt gacccacccc acccccgaag gcacctccaa tggtttgatc caactcttcg
     2641 ggcagggagc cgtggtcttc gtctacatca tggaagccat gaaaagcgct gacggttcct
     2701 tcacccctgc ccttttactt gcaatgggat tactattggc aagcctgttc tttgtttcgc
     2761 aaatgaaaga caaggctcaa tagcaaaaaa caccttatgc tatactccgt ttatgcaatt
     2821 tcaatgggat ccatccaaag cggaaagcaa tatcaaaaag catgacgtca gttttgatga
     2881 agcagttact gttttcaaag atccacttgc gttgatattc gacgataccg cccattcaga
     2941 gcgcgaattt cgagaaatta ttatcggaat gtccaccttg cgaaaaatgc ttctggtatg
     3001 ttttgtagaa aaacaggaag acataatcag gatcatcagt gcaaggccag ctacaaagac
     3061 agagatcaaa gactatgaaa aaaacgccag gtcaaaagtc aaataacata caagaaatgt
     3121 ctgcggagta tcgctttgat tataaaaaag caaagccaaa tcgttttgcc gaaaagatga
     3181 aaagcgaacc cgttattgtc atcctcgacg cagatgtggc aaaagttttc accactgcag
     3241 tcgaagttaa tacagcttta cgggcactga tctctgccat gccccccaag aagaaaaaag
     3301 ccgccgttag ataaaacacc ccttctcaaa ggggtttgtt tatgccatat gcgtcaaacc
     3361 ccatttacgg agtaatatgc cccccactct caacgacatc caacaagccg ccgagcgcat
     3421 caaaccctac gcgcaccgca cacctgtatt gactaacgaa agcctcaacc aaaaagtcgg
     3481 cgcgcaggtt tatctcaaat gcgagaacct gcaaaaggtc ggcgcgttca agttccgcgg
     3541 tgcgtgcaac gccgtgtatt ccctcacaga cgaagaagcc tcgcatggag ttgtcactca
     3601 ctcctcagga aatcacgcac aggcattggc gctcgcggcg aagatgcgcg gcatcccagc
     3661 ttatatcgtt atgccgagca acgcaccgca ggtcaaaaag gatgcggtcg ctggctacgg
     3721 cggacagatc acattttgcg agccgactct cgcggcgagg gaaagcacaa tggaggggat
     3781 caggcaaaag acaggcgcga ccgttgttca tccgtatgac aatgaaaaag tgatcgcagg
     3841 tcagggcacg gcggcaatgg aactgctgga agatgtcccc gatctcgatg tcatcatcac
     3901 gcccgtcggc ggcggcggat tgttgagcgg cacatccatt gcagcgatgg gactcaaaaa
     3961 aggaatccgc gtgatcgcgg gcgagcccga aaaagcagat gatgcgttcc gttcattgca
     4021 ggcagacagg atcatcccat ctgaaaaccc gaagacgatc gccgatggct tgctcacctc
     4081 acttggagcc ctgacctttc cgatcattca acaaaacgtc gagcagatcg tcacggtgag
     4141 cgaagaaggc atcatcacca cgatgaagtt cgtgtgggaa cgcgcgaaga tcatcatcga
     4201 accatcgtct gcggttgcca tcggcgtgct ttgggaaaac aagatcaacc tgcacggact
     4261 caagatcggc gttatcatca gcggcggcaa tgttgacctc aacaaattac cctggcagta
     4321 acggttgaaa gttgcaggtt acaagttgca agtttcatcc ttcatcc
//
LOCUS       DAOQJC010000212         4360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_12098_length_4360_cov_10.411382, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000212 DAOQJC010000000
VERSION     DAOQJC010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12098_length_4360_cov_10.411382"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..481
                     /locus_tag="PLE39_18755"
     CDS             35..481
                     /locus_tag="PLE39_18755"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNE70431.1"
                     /translation="MDTKTQLNESVKDAMKSGDELRKRTLRMVLAAVKQVEVDKQATL
                     DDMAVVALIQKEIKNRREAIEEAKKAERPDLIGDNEAEIKVLEVFLPKAMPAEELRAL
                     VSAAIAETGAAGPSDMGKVMKIVMPKVAGRAPNDMISSTVKELLAK"
     gene            539..2656
                     /locus_tag="PLE39_18760"
     CDS             539..2656
                     /locus_tag="PLE39_18760"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNE70432.1"
                     /translation="MSARTRTPVTTRIRVLQISLIIAVGLASFAALTMPIALRPSTQA
                     LDVGDVSQNTLQAPRDIEYVSDIRTEEARKAAESAVQPVYTPPDPAIARQQIERLRTT
                     LQYINSVRTNVGMTTDEKKSNLVELSDVRLKLETIDYLVGISDARWEIVQSEALRVLE
                     QVMRRAIYEDKVESTQTGIPSIVSLTLNEQQSALVAELASAFVVPNSFFSEELTIAAQ
                     QAARDAVKPIVQTYKAGETIVPAGEIITPADMEAFQQLDMIRPGQRWEDMLGAVSIIV
                     LSAVLVPLYFFRRKRAVVVNDPRSIFVIALVFVVFLVGARLFANRTLAPYGYPLQAAG
                     LLITALFGLEAGLVISIPLCLLAAYGLPNTIDLAPYYLLSSLVGLLVLGPVRRFWGFV
                     RAGIAISLTGLVVLLAYRLPFFTPDLLGVFQFIGVVSFAGFAAASVTLLLQYLLAQTL
                     GLTTAFQLLDISRPDFPLLQFFLRNAPGTYQHSLQVANLAEQAAEKIGADPLLTRVGA
                     QFHDIGKALNPTFFIENQIPGSVNKHHDIAPEESAATIIRHVTDGIQLAKKYRLPGRL
                     HDFILEHHGTLITRYQYNQAMEAANGDVTRVDIENFRYPGPRPNSRETALLMLADATE
                     ARARAERPATDDDLRALVRSVIDTVQKFGQLDDTLLTLRDLNLITESFVTTLRGTYHP
                     RIQYPNAKVPDQDSTILATRKEK"
     gene            2653..3126
                     /gene="ybeY"
                     /locus_tag="PLE39_18765"
     CDS             2653..3126
                     /gene="ybeY"
                     /locus_tag="PLE39_18765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNE70433.1"
                     /translation="MINIDNQQDFRESALLERAARLTLEISTRLDPNLEPDSVDADIT
                     IVLTDDAQLHELNREFLGVDAPTDVLSFPSDEVDPETGSQYLGDIIISIPRAAQQAQV
                     GGHSLEAEVQLLVVHGTLHLLGHDHAEAEEKARMWAAQAEVMGRLGLSHVKIQDT"
     gene            3132..3521
                     /locus_tag="PLE39_18770"
     CDS             3132..3521
                     /locus_tag="PLE39_18770"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNE70434.1"
                     /translation="MKAFFESRIASFRYAFQGWFHVLKTQRNAWIHSAIATIVFLLGL
                     WLQLPPRDWAVIILTAALVFTAEFINTSIEAVVDLASPDRHPLAKIGKDVGAAAVLVS
                     ALAAILIGLLLLGPPLWQKLTLLFSNL"
     gene            3537..>4360
                     /locus_tag="PLE39_18775"
     CDS             3537..>4360
                     /locus_tag="PLE39_18775"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNE70435.1"
                     /translation="MTLSFIFGTVGSPTGTPKKPGGSVGAIAFSKSIGLHALELGWVQ
                     SVRVTEATCAAIKSASEEQGVSLSVHAPYFINLNANAEEWPKSRKRLMDAAHYGYLAG
                     ATDIIFHPGSYFENDPLEVLKVAIPRLKGCVDELKKNGDHVTLRPETMGKSAMLGSFE
                     DAVAMSAAMDMVQPCIDFAHMHARPGDGTMNTYEEWSKLLTAYKKELGAKALKHMHIH
                     LSGIEYGPKGEKNHLPLAEADLDLKALFKALHDFGCGGRILCESPIMEEDALNMKKA"
BASE COUNT          957 a         1121 c         1220 g         1062 t
ORIGIN      
        1 tcatccaata agatgaaagg gtataatcaa ttttatggat acaaaaactc aactcaacga
       61 atcagttaaa gacgccatga agagcggtga tgaactccgc aaacgcacgc tgcgcatggt
      121 attggccgct gtgaagcagg tcgaagtgga taagcaggcc acccttgatg atatggccgt
      181 ggtggctttg atccagaagg agatcaagaa tcgccgtgag gccatcgaag aggccaaaaa
      241 ggcggaacgc ccggatttga tcggtgataa tgaggccgag atcaaggtgt tggaggtctt
      301 cctgccgaag gccatgcctg ccgaggaatt acgcgcattg gtaagcgctg ccatcgctga
      361 aaccggtgca gccgggccaa gcgatatggg caaggtgatg aagatcgtga tgcccaaggt
      421 ggctggtcgt gcgccgaatg atatgatcag ttcaactgtg aaagaattgc tcgcgaagta
      481 agcccttgcc gtgaagaggt gggtgcatgg ttgcacctgc ccctttgcgg cttattttat
      541 gtctgcacgt acccgcaccc ccgttaccac ccgtattcga gtcttacaga tcagcctgat
      601 cattgcggtc gggctggctt cctttgctgc actgacgatg ccgatcgcac tgcgtccatc
      661 gacgcaggcg ctggatgttg gcgatgtttc gcagaatacc ctgcaggctc cgcgtgatat
      721 tgaatatgtg agtgacattc gcacggagga ggcccgcaag gcggcggaaa gcgcggtgca
      781 gccggtgtat acgccgcccg atcctgccat tgcgcgtcag cagatcgaac ggctgcggac
      841 gaccctgcaa tatatcaact cagtgcggac caatgtggga atgaccacag atgaaaagaa
      901 atcgaatctg gtggagttga gcgatgtgcg cttgaagctc gaaaccattg attacctggt
      961 cggtatttct gatgcacgct gggagatcgt tcaatctgaa gcattgcgtg tgctggaaca
     1021 ggtgatgcgc cgtgccattt atgaagacaa ggtggaatcc acccagacgg gcattccttc
     1081 cattgtgagt ctgacgttga atgaacagca atcggcgctg gtggcggagc tggcaagtgc
     1141 ctttgtggtg ccgaatagtt ttttcagcga ggaattgacc attgcagcac agcaggccgc
     1201 acgtgatgcg gtcaagccga ttgtgcaaac ctacaaagcc ggggaaacga ttgtgcctgc
     1261 gggagaaatc atcacacctg cagatatgga agcctttcaa cagctggata tgatccgccc
     1321 cggtcagcgc tgggaggata tgctcggcgc ggtttcgatc atcgtccttt cggcggtgtt
     1381 ggtgcctttg tatttcttcc gcaggaagcg cgcggtggtg gtgaatgatc cgcgcagcat
     1441 ttttgtcatt gcgcttgttt ttgtggtgtt tctggttgga gcgcgcctgt ttgccaaccg
     1501 tacacttgca ccgtatggat atccactgca ggcggcgggg ttgttgatta cagccttgtt
     1561 tggccttgag gcagggctgg tcatttccat tccattatgc ttgctggccg cctatggcct
     1621 gcccaatacc attgacctgg ctccttatta tttgctttct tctcttgtgg gtttgcttgt
     1681 gctgggtccc gtgcgccgat tttggggttt tgtgcgtgca ggcattgcca tttcattaac
     1741 cgggttggtg gtgctgctgg catatcgcct gccgttcttt acacccgatc tgctgggtgt
     1801 gtttcaattt attggggtgg tgtcttttgc cgggttcgcc gccgccagtg tgactctttt
     1861 gcttcaatac ctgttggcgc aaacccttgg gctaaccaca gcatttcaac tgctcgatat
     1921 ctcgcggccc gatttcccgc tgttgcaatt ctttttgcgc aacgcgccgg gcacctatca
     1981 acacagtttg caggttgcaa acctcgccga gcaggccgct gaaaagatcg gcgctgaccc
     2041 gctattgacc cgggttggtg cgcagtttca tgatattggc aaggcgctca accccacgtt
     2101 cttcattgag aaccaaattc cggggagcgt caacaaacat catgatattg cccccgaaga
     2161 atctgcggcg accattattc gacacgtcac cgatgggatt caactggcga aaaagtatcg
     2221 cctgccgggc cgcctgcatg attttattct tgaacatcac ggcacgttga tcacacgtta
     2281 tcaatacaac caggcaatgg aagccgccaa tggcgatgtg accagggtgg atatcgaaaa
     2341 tttccgctac cccgggccgc gcccaaattc gcgtgaaacg gccctgctca tgcttgccga
     2401 tgccaccgaa gcccgtgccc gcgccgaacg ccccgccacc gatgacgatc tgcgcgcatt
     2461 ggttcgcagc gtgatcgaca ctgtgcaaaa attcggtcaa ttggatgaca cactgctgac
     2521 tctccgcgat ctgaacctca tcaccgaatc atttgtcacc acgttgcgcg gcacctatca
     2581 cccgcgcatt caatacccca atgccaaagt gccagaccag gattcaacca ttctcgccac
     2641 acgaaaagaa aaatgataaa catagataac caacaggatt ttcgggaatc agccctgctc
     2701 gaacgggccg cccgcctcac acttgagatt tcgactcggc tcgatcccaa ccttgagcct
     2761 gattctgtag acgcagacat caccatcgtt ttgacagatg atgcgcaatt gcacgaactg
     2821 aatcgcgagt tcctcggtgt ggatgctccc accgatgtgc tgtcttttcc atccgatgag
     2881 gtggatcccg aaacaggatc gcaatatctg ggcgatatca tcatttcgat tccccgcgcg
     2941 gcgcaacagg cacaggtggg ggggcactcg ctggaagcag aagtgcagct gctggtggtg
     3001 catggcacgt tgcatttgct gggtcacgat catgctgagg cggaggaaaa agcccgtatg
     3061 tgggctgccc aggccgaggt tatgggaaga ctcggcttgt ctcatgtcaa aatacaggat
     3121 acataatcaa agtgaaggcg ttttttgaat cgcggatcgc gtcgttccgt tacgcgtttc
     3181 aggggtggtt ccatgttttg aaaacccagc gcaatgcgtg gattcacagt gcgattgcga
     3241 ccatcgtttt tttgctgggg ttgtggctgc aacttccgcc gcgcgattgg gcggtgatca
     3301 ttcttaccgc ggcgcttgtt tttacggctg aattcatcaa cacttccatt gaagccgtgg
     3361 tggatctggc cagccctgat cggcatccgc ttgctaaaat tggcaaagat gttggcgcgg
     3421 cggcggtgct ggtttctgct ttggcggcga tcttgatcgg cctgctttta ctgggcccgc
     3481 ctctctggca aaaactcacc ctcctgtttt ctaaccttta atctctggag actttcatga
     3541 ccttatcgtt cattttcggt acagtgggtt cacccacagg caccccaaaa aagcccggtg
     3601 gttctgtggg cgcgatcgct ttcagtaaat ccattgggtt gcatgcattg gaactgggct
     3661 gggtgcaatc ggtgcgtgtg actgaggcca cctgtgcggc gatcaaatcg gccagcgaag
     3721 aacagggcgt ttcgttgagc gtgcatgcgc cgtatttcat caacttgaat gcaaatgctg
     3781 aggaatggcc caaatcacgc aaacgcttaa tggatgcggc acattacggc tatctcgcag
     3841 gcgcaacgga tattatcttc caccccggct cgtattttga aaatgacccg cttgaagtgt
     3901 tgaaggtcgc aattccgcgt ttgaagggct gcgtggatga attaaagaag aatggcgatc
     3961 atgtaactct tcgcccggag acgatgggca aatcggccat gcttggctcc tttgaagatg
     4021 ccgttgccat gagcgccgcc atggatatgg tgcagccctg cattgacttt gctcacatgc
     4081 acgcccgccc cggtgatggc acgatgaaca cctacgaaga atggtcgaag ctgttgacgg
     4141 cctataaaaa ggaattgggt gcaaaggcat tgaagcatat gcacattcac ctttcaggca
     4201 ttgagtatgg ccccaaaggt gaaaagaacc atcttccgct cgccgaagcg gacctggatt
     4261 tgaaggcgtt gttcaaagcg ttgcatgatt tcggttgtgg cgggcgcatc ctgtgtgaaa
     4321 gccccatcat ggaagaggat gcgttaaata tgaagaaggc
//
LOCUS       DAOQJC010000213         4353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_12140_length_4353_cov_10.806887, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000213 DAOQJC010000000
VERSION     DAOQJC010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12140_length_4353_cov_10.806887"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2066)
                     /locus_tag="PLE39_18780"
     CDS             complement(<1..2066)
                     /locus_tag="PLE39_18780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HNE70436.1"
                     /translation="MIQLFKKTSIILLVILIVLAAQPSIVAHAAPPAPIEITLYQPDG
                     FAFQARAWGDEWSNGMETLDGYTILQDGQSLFWVYAAEPTHDSLRPSTLIVGRDDPAA
                     AGLPLHLRPNVNRPAGSPPAGFQPIPEDSINVGTQRVLVLLAQFQNQPSLGTDAAYWN
                     DRIFGPALSVKKYYKEVSYGQLNLLPAIENYGTSRDGIVGWITLPYNHPNSGGNVSDH
                     NRQITRDAILASAPYVDYTVYDTNNDGYISNKELHIIVVVAGYETAFASTSSCKPGIW
                     AHYWGFEGGKVPAPVVYGKTIGEISAGGGYVQVGEWHCADLPGLTPGYPATMGVIAHE
                     FGHSLNLPDLYDTGGGSSGIGVWGIMGMGTWNTTGTQLGDLPAHHDAWSKWYEGWITP
                     LQLRGLSAGQPIPQAESNPTVFQLLDNPNGLDWKFKSNSGTGEYFLVENRQQTGFDAG
                     LPGCGLLIWHIDESVPFDNSANAGNPRRLVYLVQADGTDELGRGRDTGDAGDPFPGSR
                     WRQAFSASTTPNSNLYDGSPSSVNVLNISGCASTMTADFLTPGDGTFADIAPDYWAVD
                     SIEKLYQNGITQGCGVYPLIYCPESSTTRAQMAIFLLRSKHGSSYTPPAATGTAFADV
                     PADHWAAAWIEQLVTEGITAGCGDGNYCPESEVTRAQMAVFLLRSKYGGSYTPPAATG
                     TAFTDVP"
     gene            complement(2287..3114)
                     /locus_tag="PLE39_18785"
     CDS             complement(2287..3114)
                     /locus_tag="PLE39_18785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNE70437.1"
                     /translation="MLYKLYRKALNLYQQNPESLLARLVRMEINWLVKTLQRRQQFHF
                     PARATGGWWWIERFRFEILMRWLEVESVERVRKVVQPGMTVVDIGAHIGYYTRLLADL
                     VGPSGRVLAFEPNPENFSVLQKNMSLPRYRHVELFNYAVGERESILPLYVSPGNSNHS
                     LIEGYTKAEGVIEVRCVDLDSFLQERGIEQISFVKSDTEGAEPLVLKGMKNIAARSTN
                     LHLLMELNPTALRCGHVEPEQFLESIRSMGFEIEMITSELSAKESDANMLCSKVLKK"
     gene            complement(3123..4280)
                     /locus_tag="PLE39_18790"
     CDS             complement(3123..4280)
                     /locus_tag="PLE39_18790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE70438.1"
                     /translation="MKKLRVALYCPDHHLSYDGSTPDKQGVGGGVMVRIRLAAALAKL
                     GHQVTMICNCPRAAVFDGVQYLPLAQVRSIDVDVLILSSSGGDLDLRPALALDIKASL
                     RLIWVHGTPLLKGLHEIPFDYVCSPSNFIRQWAIQEWGVPREKTFVSYHGVDPAFLQE
                     PGLFNRRERNLFKLVYASHPSKGLDAALGVLRILRAQDARFELHIYGGNRLWGGAEQA
                     PAQEDGVFYHGMVGQRELSRELRTSHIALNLQDRLEPFGLSLIEAMASGNIVVASPVG
                     AYPEIVRDGYDGFLVEGNHMVEETWQKVAQMALTLVSAPDFSSTIRHNAQAAVRDWET
                     VARTWVGHWEWAMHVSERRMWADPCPDCGGLQLPLADGYHCASCGLYLRKG"
BASE COUNT         1018 a         1243 c         1140 g          952 t
ORIGIN      
        1 gggacatctg tgaaggcagt gcctgtggcc gcgggcggag tgtaactgcc gccgtatttg
       61 ctgcgcaaaa ggaagaccgc catttgagcg cgcgtcacct cggattcagg acagtaattt
      121 ccgtcaccac agcctgccgt gattccctca gtgaccagtt gctcgatcca tgcggcggcc
      181 caatggtcag cagggacatc ggcgaaagcc gtgcctgtgg ccgcgggcgg agtgtagcta
      241 cttccatgtt tgctgcgcaa caggaagatc gccatttgag cgcgggtggt tgagctttcg
      301 gggcaataga tcagcggata gactccgcat ccctgagtga tgccgttttg atacagcttc
      361 tcgatcgaat ccacggccca ataatcggga gcgatatctg caaaagtgcc atcaccgggt
      421 gtgaggaaat cggcggtcat ggtgctggca cagccgctga tgttcaacac attgacactg
      481 ctcggactac cgtcatataa attgctgttc ggggttgtgg aagcggagaa agcctgccgc
      541 catcgtgacc ccgggaaggg gtcgcctgca tcaccggtgt cgcggccccg gccaagttca
      601 tcggtgccgt cggcttgcac gaggtacacc agcctgcgtg gattccccgc attggctgag
      661 ttgtcgaacg gcacactttc gtcaatgtgc cagatcagca ggccgcaacc gggcaggcct
      721 gcatcgaaac cggtctgctg acgattctcc acgaggaagt attcgcctgt gcctgagttt
      781 gatttgaatt tccaatccaa gccattcggg ttatccaaaa gctggaaaac tgtcgggttg
      841 gattctgcct ggggaatcgg ctgaccagct gaaagtccgc gaagctgaag cggggtgatc
      901 cagccttcgt accatttgct ccaggcatcg tggtgtgcag gcaggtcacc caactgagtt
      961 ccagtcgtat tccaggtacc catgcccatg atgccccaaa cgccgatgcc cgatgaaccg
     1021 ccgccggtat cgtacaggtc gggcagattc aaactgtggc cgaactcatg cgcgatgacg
     1081 cccatcgtgg cggggtaacc gggcgtaagc ccgggcaggt cggcgcaatg ccactcgccg
     1141 acttgcacat atccgccgcc cgcgctgatc tcgccaatgg tttttccgta caccaccggc
     1201 gcagggacct tgccgccttc gaagccccag taatgcgccc aaatgccggg cttgcaggaa
     1261 ctggtgctgg caaaggctgt ttcataccca gccacaacca caatgatgtg taattccttg
     1321 ttcgaaatgt atccgtcgtt gttcgtgtcg tagacggtgt aatccacgta tggggctgag
     1381 gccaaaatgg catcgcgtgt gatctggcga ttgtgatcgg aaacgtttcc accggagttg
     1441 ggatggttgt atggcagtgt gatccagccg acaatgccat ctctggatgt gccgtagttt
     1501 tcaatggcag ggagcagatt gagctgtccg taggaaactt ccttgtaata ttttttcacg
     1561 ctcaaagcgg gaccgaagat ccggtcattc caataagcgg catcggtgcc cagcgacggt
     1621 tgattctgga actgcgccaa aagcaccagc acccgctgtg tccccacgtt gatgctgtct
     1681 tctggaatgg gttgaaaccc cgcaggcgga ctgcccgcag gacgattcac attcgggcga
     1741 agatgcaaag gcaggcctgc ggcggcgggg tcatccctgc cgacgatcaa agttgaagga
     1801 cgcaggctgt catgagttgg ctcagcagca tacacccaga aaagactctg cccatcctga
     1861 aggatggtgt acccatccag agtttccatg ccattcgacc attcatcgcc ccaggcccgc
     1921 gcctgaaacg cgaatccatc gggctggtac agggtgatct caatgggggc aggcggtgcg
     1981 gcatgtgcca caatggacgg ttgggccgcc agcacgatca ggatcacaag caggatgatg
     2041 gatgtctttt tgaaaagctg aatcatttgc actccaaaat tctttgtcgc cagattgact
     2101 actttacacc ttcaagatca gaattctaat tcatgctgaa aatgcagttt tttaccatca
     2161 cggcgttttc tttactggat aaaaaaacca tttgaaccac tgaggcacaa agacacggag
     2221 tctttatttg gttttctcag tgtttcagtg cctcggtggt taagatttca gaattttgca
     2281 agaggtctat tttttcaaca ccttgctgca cagcatattg gcatcgctct ctttggctga
     2341 aagctccgag gtgatcattt cgatctcgaa ccccatgctg cgaatacttt cgaggaactg
     2401 ctcaggctcc acatgtccac agcgcagggc agtgggattc aactccatga gcaggtgcag
     2461 gtttgtagaa cgcgccgcga tgttcttcat gcctttcaac accaaaggct cggcgccttc
     2521 agtatcgctt ttgacaaaac tgatctgttc gatgccgcgt tcctgcaaaa aggaatcaag
     2581 gtccacacag cgtacttcaa tcacgccttc cgccttggta tagccttcga tcaaactgtg
     2641 attgctgtta ccgggtgaaa catacaacgg cagaatgctc tcccgttcgc caaccgcata
     2701 gttaaacagt tccacatggc ggtaacgcgg caaagacata ttcttttgca gcacactgaa
     2761 attctcaggg ttcggctcaa acgccagcac acgcccgctc gggccgacca ggtctgcaag
     2821 caggcgggtg taatagccga tgtgcgcgcc gatatccacc acggtcatgc caggctgaac
     2881 caccttccga acacgttcca ccgattcgac ttccagccag cgcatcagaa tctcaaagcg
     2941 gaaacgttcg atccaccacc acccgccagt tgcccgtgcg ggaaaatgaa attgctggcg
     3001 cctttgcaga gtcttcacca gccagttgat ctccatccgc acaaggcgtg cgagcagcga
     3061 ttcgggattc tgttgataaa gattaagtgc ttttcgatag agtttataca gcattaaagt
     3121 tttcagcctt tcctgagata caaaccacat gaggcgcaat gataaccgtc tgccagaggc
     3181 agttgcagcc cgccacagtc cgggcaggga tccgcccaca tccgccgctc agagacatgc
     3241 atggcccatt cccaatgacc gacccaggtg cgtgccaccg tttcccaatc ccgcacggcg
     3301 gcttgcgcat tgtgtcggat ggtgctcgaa aaatcgggcg cactcaccaa agtcaacgcc
     3361 atttgcgcca ccttttgcca ggtttcctcc accatgtgat tcccttccac gagaaaacca
     3421 tcatacccat cgcggacaat ctccggatac gcccccaccg ggctggccac cacgatattg
     3481 cccgaagcca ttgcttcgat caaggaaagg ccaaagggtt cgaggcggtc ttgaagattc
     3541 aaagcaatgt gactggtacg cagttcacgc gacaactcac gttgaccgac catgccatga
     3601 tagaagacgc cgtcttcctg cgccggagcc tgctcagccc caccccagag acggttgccc
     3661 ccgtagatgt gcaattcaaa acgggcatcc tgcgcacgca aaatcctcaa tacgcccagc
     3721 gccgcatcca aacctttgga cggatgactt gcatacacca atttaaaaag atttcgctcc
     3781 ctgcggttga aaagacctgg ctcctgcaga aaggcagggt ccacgccgtg atagctgaca
     3841 aatgtttttt cacgcggaac gccccactct tgaatcgccc actgacggat gaaattactc
     3901 ggcgaacaga cataatcaaa tggaatctca tgcaagcctt tgagcagggg tgtgccatgc
     3961 acccagatca ggcgcagact cgctttgata tcaagagcca gagccgggcg caggtccaga
     4021 tcgccgccgc tgctgctgag aataagcaca tccacatcaa tgctccgcac ctgtgccagc
     4081 ggaagatatt gcaccccatc gaacaccgcc gcacgcggac aattgcagat cattgtgacc
     4141 tgatgcccca gttttgccag cgccgccgca agacgaatcc gcaccatgac accgccaccc
     4201 actccctgtt tatccggggt gctgccatca taggaaagat ggtgatcggg gcaatataaa
     4261 gcaacacgta atttcttcaa agagacactc ccatcttgat cgtgacagaa gcattttata
     4321 gttgattgag ggatgcgtca attgccataa aaa
//
LOCUS       DAOQJC010000214         4320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_12275_length_4320_cov_6.052052, whole genome shotgun sequence.
ACCESSION   DAOQJC010000214 DAOQJC010000000
VERSION     DAOQJC010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12275_length_4320_cov_6.052052"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="PLE39_18795"
     CDS             complement(<1..1031)
                     /locus_tag="PLE39_18795"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE70439.1"
                     /translation="MKPDFNNKVVLITGANGGLGSAFARAFTEQGAQVILAGRNLPAL
                     QSLADSMPDAPAIHTLEFDNPENHLALRQFISDSFGKVDIIVNAIGADTRKLLEEHQQ
                     DEIHKLLSANLHGAILLTQTFLPLMKSQNDGMIVHIGGFADGHLAFPFYSVDAATRAG
                     MFTFIESVNRELALEKSNVRIGYFSPSPADTEAERPYHAIWQEMGVKILPVEKIASEL
                     LSMIANRHMVHIMGGFSTRLFAGINAISPRLADLILLNRYSAILKKHLIDKNPTPPPP
                     AAWMFPLGIGLVILSTLLYGGLIALPFFSIPTTTKLAATPVLIFFGEATFWVGGALLG
                     KEIVTRYKQ"
     gene            complement(1028..1615)
                     /locus_tag="PLE39_18800"
     CDS             complement(1028..1615)
                     /locus_tag="PLE39_18800"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE70440.1"
                     /translation="MPKPDVSAERIPLILNSAIKVFSKKGFEAARMEDIAKTAKLSVG
                     GVYWYYKSKEEIIIAIMVQLIDKDVKDLRALLEAEGMVRARLETYIGLSIEAAQEYIP
                     ITYELYSLAHRNAKVKNHIRAFLQTYHGVLEQFIQQGIDRKEFKSVNAREAALTLAAL
                     YEGALELNMLDPERVDAKQTLLSSIKLLMNGLENK"
     gene            1800..2357
                     /locus_tag="PLE39_18805"
     CDS             1800..2357
                     /locus_tag="PLE39_18805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNE70441.1"
                     /translation="MDDLQAVRRIKEGDWSGLEVLMERYQVRAVRAAFLILHDESAAQ
                     DVVQDTFIRICRRIHQFNEEHAFEPYFFGSVIHAALNATRGNHKFVSLDEKSEEIEHL
                     LDCAVSAESQVEFLQFQHEILTALSELSPRQRAAIVQRYYLEMSEKEMAFAMQAAPGT
                     IKWLLNAARDRLRRLLGPKGESNDG"
     gene            2464..>4320
                     /locus_tag="PLE39_18810"
     CDS             2464..>4320
                     /locus_tag="PLE39_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HNE70442.1"
                     /translation="MQALRTRPILVVALFVVVILLLNGVAYAVDRFVGYIPGFGFTSG
                     SQAVLVLDAPVSQTHNGITLRVDQAISDGDRFWVEMTVSGYSFFETQDHRDAYPNPDT
                     GEYPPAFLVLPSGEKIQSLFGESPNLGAEEILFRYEFPPLKETPPLLTLVVEKMGGET
                     FVMPLKMRPIHQSEIVPAQPEENTPVKSVTHDGLTFVMEKVAQSSTETFFEFTLQADD
                     PRLSLLSPWSVLVTDQHGNIYPLITHTKASVYLEGAKTYKSAPFHGDEQLTMRLMGEI
                     FTRGKLPAQYEYLIDELSPSFHFDPGSAPQVGQTWQLDETLTGNGFTLHLVGARVASE
                     HEIIFEIEHSTNVTRIAFRAEPPAQTWADVPPDGGNIQLRVGYRSLPTAPYELSLSTV
                     MFMLEENWEIKWIPQPAPQPSGDALLSTPTPGTPPEIHATPTLTSTAPFLLEMEALSQ
                     KFNAQFKTGPAWIHVVWEGGQYQTPGLLNYPPAYYTIEKWMEIDKDGLIIRELNTDRD
                     VDEMILQQNVSMNGYGVNLTQGNGGAMSSNTLYQFTEDYIVEVLDRAYQNGQSVKHEE
                     TTCEGRPCWLIWIQSDVETFRHPVIQKMVVGNIQKIWMNFQTGRLIRSESIWQ"
BASE COUNT         1073 a          983 c         1117 g         1147 t
ORIGIN      
        1 tattgtttgt aacgcgtgac gatttctttg ccgagcaatg cgccgccgac ccagaaggtg
       61 gcttcgccga aaaagatcaa cacaggcgtg gcggcaagtt tggttgtggt gggaattgaa
      121 aagaacggca gcgcgatcaa gccgccatat aacagcgtgg agagtatcac cagaccgatg
      181 cccagcggga acatccatgc ggcgggcggt ggcggcgtgg gatttttatc gatgagatgt
      241 tttttcaaaa tggcgctgta acggttgagc agaatgagat ccgcaaggcg cggcgagatt
      301 gcgttgatgc ctgcgaagag gcgtgtgctg aacccgccca tgatgtgaac catgtggcgg
      361 ttggcgatca tactgagaag ttctgaagcg attttttcaa cgggcaggat cttcacgccc
      421 atctcttgcc agatggcgtg atagggacgc tccgcttcgg tgtcggcggg gctgggggag
      481 aagtagccga tgcgcacgtt gcttttttcg agcgcgagtt cgcgattgac agactcgatg
      541 aatgtgaaca tgcctgcccg tgtggcggca tccacactgt agaaggggaa ggcgaggtgt
      601 ccatcggcga acccgccaat gtggacgatc atgccgtcgt tttggctttt catcagcggt
      661 aggaaggttt gggttagtaa gatcgctccg tgtaagttgg cgctgaggag tttgtgaatc
      721 tcatcttgtt gatgttcttc cagcagcttg cgggtatctg cgccaatggc attgacgatg
      781 atatccactt tgccaaacga atcagaaata aattgacgca aagccaaatg attttcgggg
      841 ttgtcgaact caagcgtgtg gatagcgggt gcgtcaggca ttgagtccgc gagcgattgc
      901 aaggcaggaa gatttctacc cgctagaatc acctgcgctc cttgttctgt gaaggctcgt
      961 gcaaaggcag agccaagtcc gccgtttgca cctgtgatga gcacgacttt gttgttgaaa
     1021 tcaggtttca tttgttttcc aatccgttca taagaagttt gatcgaactg agcagggtct
     1081 gtttggcatc cactcgttca gggtcaagca tgttcaattc caatgcgcct tcgtagagtg
     1141 cggcgagggt taacgcagct tcacgggcat tcacagactt gaactctttg cggtcaatgc
     1201 cttgttggat gaattgttcc aacacgccgt gataggtctg caaaaaggcg cggatgtgat
     1261 tcttcacttt cgcgttgcgg tgtgccagtg aatacaactc gtaagtgatg gggatgtact
     1321 cttgcgccgc ttcaatactc aacccaatgt aggtttccag ccgcgctcga accatccctt
     1381 cggcttcgag cagcgcacgc aggtctttca catccttgtc gatcaactgc accatgatgg
     1441 cgatgatgat ctcttccttg cttttgtaat accaatacac gccgcccacg ctgagtttgg
     1501 cggtcttggc aatatcttcc atgcgggcgg cttcaaagcc ttttttggaa aagaccttga
     1561 tggcggaatt tagtatgagc ggaattcgct cggcgctgac atcgggcttg ggcatgcttc
     1621 ctcaaaataa ctgaatttat attcggttat tttatcaatt tgtatttata tgtcaagatg
     1681 atatgctgta tgaatttagc tggaaattga actggaggat ttaggtgttg aggacccaac
     1741 cttttcgttt atgattgtgt atacctgata tgggagcaaa aaggacaagg gaaaacttca
     1801 tggatgattt gcaggccgtt cgccgcatta aggaaggcga ttggagcggg ttggaagttt
     1861 tgatggaacg ctatcaggta cgagcggtgc gggccgcatt tctgattctg catgatgaat
     1921 ctgccgcaca ggatgtggtt caggacacat ttatccgcat ctgtaggcgg attcaccaat
     1981 ttaacgaaga acatgccttt gagccttatt tttttggaag tgtgattcat gcagctttga
     2041 atgccacacg gggaaatcat aaatttgtat ccctggacga aaaatcagaa gaaatcgagc
     2101 atttgctcga ctgtgcagtt tcagcggaat cgcaagtgga gtttttgcaa tttcagcatg
     2161 agattttgac tgcattgtca gagctttctc ctcgccagcg ggcagcaatt gtccaacgat
     2221 actatttgga gatgagcgag aaggaaatgg cttttgccat gcaggccgcc ccaggaacga
     2281 tcaaatggct attgaacgca gcccgtgaca gattacgccg cctgcttggg ccgaaaggtg
     2341 agtcaaatga tggctaaaaa gactcgtcat gaagtattgg attcaatcgc cgctctgcgt
     2401 gtttcgcaca gccttgatct ctttccacac gttattaaaa agttgaatca aaggagggat
     2461 ctgatgcaag ctttgcgcac tcgtccgatt cttgtagtag ccctgtttgt agtagtcata
     2521 ctattgttga atggtgttgc ctatgccgtt gaccgtttcg ttgggtatat tccaggtttt
     2581 ggattcactt ctggctcaca agctgttttg gtattggatg cgcctgtgtc gcaaacccac
     2641 aatggcatta ctttacgggt ggaccaagct atcagcgatg gtgacaggtt ctgggttgag
     2701 atgaccgtga gcgggtattc gtttttcgag acacaagatc atcgggacgc ctatccaaat
     2761 cccgatacag gagaataccc gcctgcgttt cttgtgcttc caagcggaga aaaaatccaa
     2821 agcctgtttg gagaaagccc aaatttgggg gctgaagaaa ttcttttccg atatgaattt
     2881 ccgcctttga aagaaacacc tcccttgttg acattggtcg tggaaaagat gggcggtgaa
     2941 acctttgtca tgccgttgaa aatgcgtccg atccatcaga gcgagatcgt ccctgcacag
     3001 cctgaagaga acacccctgt taaaagcgta acccatgatg ggctgacatt tgtcatggaa
     3061 aaggtggcgc aatccagcac tgaaactttt tttgaattta ccttgcaggc tgatgatcca
     3121 cgcttgagtc tgctttctcc atggagcgtt ctggtcactg accagcatgg aaatatctat
     3181 ccgctgataa cacataccaa ggcttcggtg tacctggagg gtgcaaagac ttataaatct
     3241 gcgccatttc acggtgatga acaattgacc atgcgtttga tgggcgagat ctttacccgt
     3301 ggaaaattac ccgctcaata cgaatatctg attgatgaac tatcaccctc atttcatttt
     3361 gaccctggct ccgctccaca ggtcggacaa acctggcaac ttgatgaaac cctgaccggc
     3421 aatggcttta ctttacattt agtcggcgcg cgcgtggcat cagagcatga aatcattttt
     3481 gaaatcgaac actccacaaa tgtgactcgt attgcttttc gggcggaacc acctgctcaa
     3541 acctgggccg atgtaccacc agatggaggg aatatccagc ttcgcgtggg gtaccgatct
     3601 ttgccaactg caccctatga actcagcctt tcaactgtga tgtttatgtt ggaagaaaac
     3661 tgggaaataa aatggattcc acaaccagcc cctcaaccat caggtgatgc gctcctctcc
     3721 accccaacgc ctggcactcc tcctgaaatc cacgccaccc ccacattgac ctcaaccgcc
     3781 ccattccttt tggaaatgga agccctctct caaaaattca acgcccaatt taaaactggc
     3841 cctgcatgga ttcatgttgt ttgggaaggt gggcaatatc aaacccctgg gctgctaaac
     3901 tacccgcccg catattacac aattgagaaa tggatggaga tcgataaaga cggtttgatc
     3961 attcgtgaac taaacaccga cagggatgtt gatgagatga tcctccaaca aaatgtcagt
     4021 atgaatggtt atggagtcaa tcttacacag gggaatggcg gcgcgatgag ttccaatact
     4081 ctgtatcaat ttacagaaga ttacattgtt gaggttcttg accgtgccta tcaaaatgga
     4141 caatctgtaa aacatgagga aaccacctgt gaaggacgac cttgctggtt gatctggatt
     4201 cagtctgatg tggaaacatt tcgtcatccc gtcatacaaa aaatggttgt gggcaatatc
     4261 caaaagattt ggatgaactt tcaaactggg aggcttattc gctctgaaag tatttggcaa
//
LOCUS       DAOQJC010000215         4269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_12504_length_4269_cov_9.565971, whole genome shotgun sequence.
ACCESSION   DAOQJC010000215 DAOQJC010000000
VERSION     DAOQJC010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12504_length_4269_cov_9.565971"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLE39_18815"
     CDS             complement(<1..405)
                     /locus_tag="PLE39_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70443.1"
                     /translation="MKKYILLLLTLTLLIPTASVLAEGQFDYIVIRGFGIVEDINISN
                     PALTQDYYAFADFSKGEVNPPSDPGIGYQVVRMYAKDSKGIPYDQLHYYPDSGYVFYD
                     GIVNGYSELGTKWYLANPTVEEPFRAALAENAR"
     gene            complement(538..1620)
                     /locus_tag="PLE39_18820"
     CDS             complement(538..1620)
                     /locus_tag="PLE39_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70444.1"
                     /translation="MKLNHYPVPFLFLAILGLLAALWAGLMRLGWQLPALTPSLAMLH
                     GPVMISGFLGTLITLERAVAMKQKWMYLPPLLSGLGWLVATILPKLPFGMVLLTLASL
                     GGVAILAEIARREFALHTVTMLLGAIAWLTGNLLWMFGWQINQVVFFWMAFLVLTIAG
                     ERLELSRVLRPTQMQQILFGSIVFIFLAGIILSLFNLQFGTRLSGLGLLLFLLWSLRN
                     DIAWRNIRHKLPLTRYIAWCLALGFLWLGISGGLSLGLGAQVAGPRYDAMLHTVFVGF
                     VISMIFGHAPIIFPAILGIPINFQPVFYIHLGLLHISLLLRVIADYANLFTLRMWGGL
                     LNEIAIFIFISLTVVSVRKGMIGKAR"
     gene            1834..4215
                     /locus_tag="PLE39_18825"
     CDS             1834..4215
                     /locus_tag="PLE39_18825"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNE70445.1"
                     /translation="METDVTSTIDVNLPRSISVRSLVEFVMQSGDLSTGGFQRRDRAQ
                     LGTQGHKRVQRSRPEGYETEVEVRCQINSGDMPLEVRGRIDGLYASHEPVIIEEIKTT
                     SLSLDLVSEDHNPLHWGQAQCYAYMYARQHRLDEVSIHLTYYHLDSHEEKTFVRHFTW
                     TGLEDFFSGLITSYLDWFRKVQAWQLQRDRSIEKLEFPYEEYRPGQRDMAVAVYKAIR
                     AKDRAYIQSPTGVGKTIATLFPSVKALGQGLATKIFYLTAKTSGRLVAEKALEDMRQS
                     GLYLRSVTLTAREKICFCPPVNCDPQVCIFARGYFDKVKSALAELAQHQTFTRPLIEE
                     IARKYEICPFEFSLDLALWVDCIICDYNYVFDPRVYLHRFFDFTTEPYIFLVDEAHNL
                     PDRARAMYSAELEKEKVLQLQRALKPSAPGLAKKLGEVNKLLLEKRKTCEGEGRDVLI
                     EHELPEALLKAIREFVNKAEDWLVQNQVAEFRQELLEFYFLCSNYLRTADYFDTFYVS
                     YFERVGQSNLRAKLFCLDPAPMLTVPLERSRSTIFFSATLLPMDYFMKLLTGAAEHPR
                     RIFHSPFPQENVCLLVHNKISTKFVHRADSYDAIAEAIETVLNTHVGNYLVYFPSYVY
                     LNEVVERLKGRLPAEQLLVQERGMTEEARDAFLAQFSSANEETLVGFAVMGGVFGEGI
                     DLVGERLIGVVVVGVGLPQLGLERDLIKGYFDHHSASGFAYAYQYPGFNRVLQATGRV
                     IRTDTDQGIIVLIDERFAQAHYRQLFPSHWRDYKIVKNTQEMKDHLTRFWSQK"
BASE COUNT         1139 a          978 c         1126 g         1026 t
ORIGIN      
        1 ccgcgcattt tcagccagcg ccgcgcggaa aggttcttca accgtggggt tggcaagata
       61 ccatttcgtg cccaattcag aatatccatt cacgatgccg tcgtaaaaaa catatccgct
      121 gtcggggtaa taatgcaatt ggtcgtaggg aatgcccttg ctgtctttgg catacatacg
      181 gacgacttga taaccaattc cagggtcgga gggcgggttg acttcgcctt tggaaaaatc
      241 agcaaaagca taataatcct gtgtcagcgc agggttggat atgttgatat cttcgacaat
      301 tccaaacccg cgaatgacga tgtagtcaaa ttgtccctcg gcaagaacgg aggcggtggg
      361 aatcagcaag gttagagtta ataagagcaa gatatatttt ttcataagtc tccagtttta
      421 atttggtcta ttgtatgtga aaacctgtcc gcttaatagg gttgtgattg aattatccct
      481 gaaataaaaa attcatcttt gcgcttaagc aaagatgaat cgagatttgc ttgaaaatta
      541 tctggctttt ccgatcatgc cttttcgcac tgagacaacc gttagtgaaa taaaaataaa
      601 aatggcaatc tcatttaaca atccacccca catacgcaat gtgaagagat tggcgtaatc
      661 agcgatcacg cgcagcaaca gcgaaatgtg caaaagcccc aagtggatat aaaatacagg
      721 ctgaaagttg atcgggattc ccaaaatagc aggaaagata atgggggcgt gaccaaagat
      781 catagaaatg acgaagccaa caaaaaccgt gtgaagcatc gcatcatacc ttggacctgc
      841 aacctgcgcg ccaagcccca ggcttaatcc gccactgatg ccgagccaaa gaaagccaag
      901 tgcgaggcac caggcaatgt agcgcgtgag cggaagtttg tgccgaatgt tgcgccaggc
      961 gatatcgtta cgcagcgacc aaagcaggaa caacagcaag cccagtccgc tcaggcgtgt
     1021 gccgaattgc agattaaaca gggataggat aatgcctgcc agaaaaatga acacaatact
     1081 gccaaagaga atctgttgca tttgtgtagg acgcaaaaca cggctgagtt cgagccgttc
     1141 acctgcaatg gtcagcacaa ggaaagccat ccagaaaaat acaacttgat tgatctgcca
     1201 gccgaacatc cataaaagat ttcctgtcag ccatgcaatt gcaccgagta acatggtgac
     1261 agtgtgtaat gcaaattcgc ggcgggcgat ctccgccaga atggcaaccc cgccgagact
     1321 agcaagggtt aggagaacca tgccgaaagg aagcttcggc aaaatggtgg caaccagcca
     1381 acccaatcca gaaagaagcg ggggcaggta catccacttt tgtttcattg ccacggcacg
     1441 ttccaatgtg atcaatgttc ccaaaaatcc agagatcatc acgggaccat gcagcatggc
     1501 aagggacggg gtgagagcgg gcaattgcca gccaaggcgc atgagacctg cccatagtgc
     1561 agcgagcaga ccaaggatgg cgagaaagag aaaggggaca gggtagtggt tgagtttcat
     1621 tttaatgaag ggctttcgtg ttgtctgccg atagttgttg aaggatgctt gattatatta
     1681 tcaataacct attattgact gcgctttgaa ggtgaaaagc gtaaatcgta cctccttcag
     1741 ccaggtgcgg accaacgtga cttgggaagg ttgtaaaaaa gccttccaac atgggttgac
     1801 ccagttcaac tcatgctaga attccagatg caaatggaaa ctgatgtcac ctctacgatc
     1861 gatgtaaacc tgccgcgaag catctcggtt agaagcctgg tggaatttgt gatgcaatcc
     1921 ggggacctgt ctacgggcgg atttcaaagg cgggaccgtg cccaacttgg aacgcaggga
     1981 cataagcgag tccagcgttc ccgccccgaa ggttatgaaa ctgaagtgga ggttcgctgc
     2041 cagatcaaca gcggtgacat gccattagaa gtgcgggggc ggatcgatgg cctgtatgcc
     2101 agccatgaac ccgtcatcat cgaggagatc aaaacaactt cactaagcct ggatctggtg
     2161 agtgaagacc ataatccgct gcattgggga caggcgcaat gctatgccta catgtatgcc
     2221 cggcagcatc gactggatga agtcagtatt cacctgacct attatcattt ggatagtcat
     2281 gaagaaaaaa cctttgtacg ccattttaca tggacaggtt tggaggattt tttctcgggt
     2341 ttgatcacct cgtatctgga ttggtttcgc aaagttcaag catggcaact ccagcgggat
     2401 cgttccattg agaaacttga atttccctat gaagagtacc gccccggtca gcgggatatg
     2461 gcggttgccg tgtacaaggc cattcgggcg aaggatcgtg cttatatcca gtcgccgacg
     2521 ggggtgggga aaacgattgc gaccctcttc ccatcggtca aagcgctggg gcagggcctg
     2581 gcaaccaaaa ttttttacct gacggcaaag acatcggggc ggctggtggc ggaaaaagcg
     2641 ctggaggata tgcgtcagtc ggggttgtat cttcgcagtg tgacactgac cgccagggaa
     2701 aagatctgct tttgtccccc ggtcaattgc gacccgcaag tgtgcatctt cgcccgtgga
     2761 tattttgaca aagtgaaaag cgcgctggca gaacttgccc agcatcaaac atttacccgc
     2821 ccgctcattg aagagatcgc ccgaaagtat gagatctgtc catttgagtt ttcgctggat
     2881 ctggccttgt gggtggattg catcatctgt gattacaact acgtttttga cccacgggta
     2941 tatctccacc gttttttcga tttcaccaca gagccgtata tctttcttgt tgatgaagcg
     3001 cataatcttc cagaccgtgc cagggccatg tactcggctg aactggaaaa ggaaaaagtg
     3061 ctgcaacttc agcgcgcctt gaaaccatct gcaccggggc tggcaaaaaa gctgggagag
     3121 gtcaacaagc tattgctgga aaagcgcaaa acctgtgaag gggagggcag ggatgtgttg
     3181 atcgagcatg aactccctga agcgctgctc aaggcgattc gagagttcgt taataaggcg
     3241 gaagactggc tggtacagaa tcaggtggca gagtttaggc aggagctgct ggaattttat
     3301 ttcttgtgca gtaattatct gcgcactgct gattattttg acactttcta tgtgtcttat
     3361 tttgagcggg tggggcaatc aaaccttcgg gcaaagttat tctgcctcga tccagcaccc
     3421 atgttgacgg tcccgttgga acgaagccgc tccaccatct ttttctcagc cacattgctg
     3481 ccgatggatt attttatgaa actcctgaca ggcgctgcag aacatcccag gcgcattttt
     3541 cattccccct tcccgcagga gaatgtctgt ttgttggtcc acaacaagat ctccaccaaa
     3601 tttgtgcatc gggctgattc ttacgatgcc attgccgaag ccattgaaac cgtcctcaat
     3661 actcatgtgg ggaattatct ggtgtacttt ccgtcttatg tctatttgaa tgaggtggtg
     3721 gaacggctca aagggagatt gcccgcggag cagcttctcg ttcaggaacg cgggatgacg
     3781 gaagaggctc gtgatgcatt tctggctcaa ttttcttcag ccaatgaaga aacactggtc
     3841 ggcttcgctg tgatgggtgg agtttttggc gaagggatcg atctggttgg agaacgattg
     3901 atcggggttg tggtcgttgg cgtggggttg ccccagttgg gattggaacg ggacttgatc
     3961 aaagggtatt ttgatcacca ctctgcaagc ggatttgcct atgcctatca atacccgggg
     4021 ttcaatcggg tattgcaggc aacgggtcgg gtcattcgta cggatacaga tcagggcatc
     4081 attgtgttga tcgatgaacg ttttgcccag gctcactacc gccaattgtt tccttctcat
     4141 tggcgcgact ataaaattgt aaaaaacacc caggagatga aagatcattt gacaagattt
     4201 tggtcacaga agtgagcttg acacctccga gtagttcagc cctaaaagcc tgcaatgccc
     4261 ccaactttt
//
LOCUS       DAOQJC010000216         4222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_12718_length_4222_cov_10.711783, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000216 DAOQJC010000000
VERSION     DAOQJC010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12718_length_4222_cov_10.711783"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..2224
                     /gene="ppk1"
                     /locus_tag="PLE39_18830"
     CDS             104..2224
                     /gene="ppk1"
                     /locus_tag="PLE39_18830"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNE70446.1"
                     /translation="MEKEFLEAQSELNDPSLFLNRELSLLEFQRRLLDEAKDERNPLL
                     ERVKFLAILGSNLDEFFMVRVSGVRRQVEAGVTELSIDGMTPRDQLAAMRKFASELYK
                     EAHTCLTKKLMPKLDKAGIHVMEYEKLTKVQKEKVDAYFREMIHPVLTPLALDPGHPF
                     PHISNLSLSLAVVIHDPKGNERFARLKVPDTLPRLVPIKRSSGGERKDGTIPRHHYFV
                     WLEQVIAANIEMLFPGMHVVSAHPFRTIRDADVEIQEIEADDLLETMQQSIRRRKFGS
                     VVQITVNEAMPEHIRELLVDNLEAKPNDIAVQSNPLGLASLWQLYKSVEKHELKYVPY
                     TPRIVKALKNLESTEDFFAAIRQQNILLHHPYISFSSVVDFLNAAARDPHVLAIKQTL
                     YRVGNNSPIVEALLEASERGKQVAVLVELKARFDEESNISWARKLEQAGVHVVYGLMG
                     LKTHCKVGLVVRQEGEGIRRYVHLGTGNYNAVTANLYEDLGMFTADETIGTDTTDLFN
                     FLTGYSAQQTYRKLLVAPVCLREKFEALILREIEHAKNGRKARLIFKANAIVDPRIIQ
                     LLYRASQAGVKVDLIIRGVCCLRPGVKGISENIRVISVVGRYLEHSRIYYFQNDGAEE
                     IYLGSADLMSRNLNRRVEVAFPIEDAEHIRRIRDTILDTYLHKNARVRFMQSNGDYII
                     NTSKDGHTEDIQEALINEYRNRRE"
     gene            complement(2228..3454)
                     /locus_tag="PLE39_18835"
     CDS             complement(2228..3454)
                     /locus_tag="PLE39_18835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE70447.1"
                     /translation="MMNLFPWVMAGLLLLVAAWFAWRYSVLRRRINEYSIVVRDHSGN
                     LPTDFKSLETLSNTVASLKTTFDLQFSTLNSQNTRLATVLSQLTDGVLIADSMGQVQY
                     ANPAAQKLFESENPIGRSVAEVVRNHQLIEAWRRCQQVRELQTESVELPARKYFLQLI
                     VIPDEHAGGSLLVVQDLTRVRKLETVRRDFISNVSHELRTPLASLKALTETLQNGAIA
                     DPEAGPRFLSRITTEVDALTQMAQELLDLSRIESGQVGLVLAPLSPKDLLSSAADRMK
                     LQAERANLKLSANCPAGLPAIRADQSRLEQVLVNLIHNSVKFTKPGGEIVLEAEAGFS
                     CVRFAVRDSGVGIPSESLSRIFERFYRVDRSRTGSGTGLGLSISKHIVEAHGGRIWAD
                     SEETRGSTFYFEIPVW"
     gene            complement(3469..4179)
                     /locus_tag="PLE39_18840"
     CDS             complement(3469..4179)
                     /locus_tag="PLE39_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE70448.1"
                     /translation="MAETILVVEDELSLQETLAYNLKKEGYTVETVGDGRSALESARR
                     LKPDLIVLDIMLPEMDGFEVARILRKEMSTAILMLTARDDEIDRVVGLEVGADDYLTK
                     PFSMRELMARVKSQLRRARLLREEMGRPEASPPRHEALKFDNLVINLTRREVTLDETP
                     LQLKPKEYELLLFFAEHKGQMLSREFVLERVWGWDFIGDSRTVDVHIRWLRQKIEKDS
                     ANPVRIVTVRGGGYRFEG"
BASE COUNT         1042 a         1170 c         1067 g          943 t
ORIGIN      
        1 ctgcttacaa cggatgcatt agaatatgga aagtgtgcca gcctgaactg gctgctatcc
       61 ccttcacagt tggtgcaaat tggcaaaaac tcataagcaa agcatggaaa aagaatttct
      121 cgaagcccag tcggagttga atgacccgtc actgttcttg aaccgcgaac tcagcctgct
      181 ggagtttcaa agacgcctgc tggatgaggc caaagatgag cgcaacccat tgttggagcg
      241 ggtgaaattt ctcgccatcc tcggttcaaa ccttgatgaa tttttcatgg tgcgcgtttc
      301 gggtgtccgc aggcaggtgg aggcgggggt caccgagctt tcaattgatg ggatgacacc
      361 ccgcgaccag cttgccgcaa tgcgtaaatt tgccagtgaa ctctacaagg aagcccacac
      421 ctgcctgacc aaaaagctga tgcccaaact cgataaagcg ggcattcatg tgatggaata
      481 cgaaaaatta acaaaggtcc aaaaggaaaa ggtggatgcc tatttccgcg aaatgatcca
      541 ccctgtctta actccgctgg cgcttgatcc cggccacccg tttccgcata tttcaaacct
      601 gagtttgagt ctggctgttg tcatccacga cccgaaaggg aatgaacgct ttgcgcgtct
      661 caaagtgcca gatacacttc ctcgcttggt acccatcaaa cggtcgtcgg gaggcgagcg
      721 caaggatggc accattccac gccaccacta ttttgtctgg ctggaacagg tcatcgccgc
      781 aaatatcgaa atgcttttcc cgggcatgca tgtggtttct gcgcatccat tccgcaccat
      841 tcgcgatgca gatgtggaga tccaggagat cgaggccgat gacctgctcg aaaccatgca
      901 gcaaagcatc cgccgcagaa aattcggctc ggtggtgcag atcacagtta atgaagcgat
      961 gccggagcac atccgcgaac tgttggtgga taacctcgaa gccaagccaa acgacatcgc
     1021 tgtgcaatcg aatccgctgg ggctggcttc cctttggcag ctttacaaga gcgtggaaaa
     1081 gcatgaactg aaatacgtgc cctacacccc caggatcgtc aaagcgctga aaaatcttga
     1141 gtccactgaa gatttctttg ccgccatccg ccagcagaat atcctgctgc atcaccccta
     1201 tatttcgttt tcctcggtgg tcgattttct aaacgcggcg gctcgtgatc cgcatgtttt
     1261 ggccatcaaa cagaccttgt atcgagtggg caataactca cccattgtgg aagccctgct
     1321 cgaagcctca gaacgcggca aacaggtggc tgtgttggtg gagctcaaag cccgctttga
     1381 tgaagaaagc aacattagct gggcacgcaa actggaacag gccggtgtac atgtggtgta
     1441 cggtttgatg gggctgaaga cacattgcaa agttggcctt gtggtacggc aggaaggtga
     1501 aggcattcgc agatatgttc acctcggcac cggaaactac aatgcggtca cagccaacct
     1561 atatgaagac ctcggcatgt ttactgccga tgaaaccatc ggcaccgaca ccaccgactt
     1621 gttcaacttt ctcacgggtt actctgccca gcaaacgtat cgcaaattgc tggttgcacc
     1681 ggtatgcctg cgcgaaaaat tcgaagccct gatcctgcgc gagatcgaac atgcaaagaa
     1741 cggcagaaag gcacggctca ttttcaaggc caatgccatc gtagacccgc gcatcattca
     1801 actgttgtac cgcgcctcac aggcgggagt caaagtggat ctgatcatcc gcggggtgtg
     1861 ctgtctgcgc cccggcgtta aaggaatcag cgagaatatc cgtgtcatca gtgtggtcgg
     1921 acgatatctt gaacacagcc gcatttatta ttttcaaaac gacggcgccg aagagatcta
     1981 ccttggcagc gccgacttga tgtcgcgcaa tttgaatcgc cgtgttgaag tggccttccc
     2041 catcgaagat gctgaacaca tccgccgcat ccgcgatacc atcctcgaca cctacctgca
     2101 caaaaacgcg cgcgtacgct tcatgcagag caacggcgat tacatcatca acacatcgaa
     2161 agacggtcac accgaagata tacaggaagc cctgatcaac gaataccgca acaggcgcga
     2221 ataattttca ccagacaggg atctcaaaat agaacgtgct gccgcgtgtc tcttcgctat
     2281 ccgcccagat ccttccgcca tgcgcctcca caatatgctt cgagatcgaa agcccaagcc
     2341 ccgtgcccga acctgtgcgg gaacgatcca cgcgatagaa tctttcaaag atgcgggata
     2401 gactctccga agggatcccg actcccgaat cccgcacagc gaatcgtaca cagctgaaac
     2461 ctgcctctgc ttcgaggacg atctctcccc caggctttgt gaacttcacc gaattgtgaa
     2521 tcagattcac caatacctgc tccagccgcg attggtccgc gcggatggcg ggcaggcctg
     2581 cagggcagtt tgcgctcaac ttcaagtttg cgcgttccgc ctgcaatttc attcgatctg
     2641 ccgcagaaga caacaaatcc ttcggtgaca acggcgcaag gaccaacccc acctgccccg
     2701 attcaattct ggaaagatca agcagttcct gcgccatttg cgtcagcgca tccacttcgg
     2761 tggtgatcct gctcaagaaa cgcggacccg cttcagggtc tgcaatggcc ccgttttgca
     2821 gggtctcagt caatgctttg agtgatgcaa gcggggtccg cagttcgtgt gagacgttcg
     2881 aaatgaaatc cctgcgcacg gtttcaagtt tacgcacccg ggtcaggtcc tgcaccacca
     2941 gcaggcttcc acctgcatgt tcatcgggaa tgacgatgag ctgcaaaaaa tattttcggg
     3001 cgggcaattc aacagattcg gtctgcaact cgcgcacctg ctgacagcgg cgccatgcct
     3061 cgatcaattg atggttgcga accacttcag ccacactgcg cccaatgggg ttctctgact
     3121 cgaaaagttt ttgcgctgca gggttggcat actgcacctg tcccatcgaa tccgcgatga
     3181 gcacgccgtc ggtcaattgg gaaaggacgg ttgcaaggcg ggtgttttgt gagtttaaag
     3241 ttgaaaattg aaggtcgaag gttgttttga gcgaagcaac tgtgttggaa agggtttcaa
     3301 gggatttgaa atcggtgggc aaatttccag aatggtcgcg gacgacgatc gaatactcat
     3361 taatacgtct tcttaaaaca gaatatcgcc acgcaaacca tgcagcgacc aaaagcaaaa
     3421 gaccagccat tacccacgga aatagattca tcatcgctca tttccccgct atccttcaaa
     3481 cctgtacccg ccgccgcgca cggtcacgat ccgcaccggg ttggcagagt ctttttctat
     3541 cttttggcgc agccagcgga tatgcacatc cacggtgcgg ctatcgccga taaaatccca
     3601 gccccacacg cgttccagaa caaattcacg cgaaagcatc tggcctttgt gttcggcaaa
     3661 gaacagcagc aattcatatt cttttggttt taattgcagg ggggtttcat ccagcgttac
     3721 ttcgcggcgg gtaaggttaa tgacaaggtt atcgaatttc aaggcctcgt gcctgggggg
     3781 tgaagcctcc ggcctgccca tttcctcacg cagcaagcgc gccctgcgaa gctgcgactt
     3841 cacccgcgcc atcaactcac gcatggagaa cggtttggtc aggtagtcat ccgccccgac
     3901 ttcgagcccc accacacggt caatttcatc gtcacgggcg gtgagcatca aaatcgccgt
     3961 ggacatttcc ttgcgtagaa tccgtgccac ttcaaagccg tccatttcgg ggagcatgat
     4021 atcgagcacg atcaggtcgg gtttcaagcg ccgcgccgat tcaagcgcag atcgtccatc
     4081 ccccaccgtt tccaccgtgt acccttcttt tttcagattg taggcaaggg tttcctgcaa
     4141 agagagttca tcttcaacaa caaggattgt ttcagccatt gctgggaata tatcacaaag
     4201 ggttggctgt ctgttaatgg ga
//
LOCUS       DAOQJC010000217         4215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_12752_length_4215_cov_10.169952, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000217 DAOQJC010000000
VERSION     DAOQJC010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12752_length_4215_cov_10.169952"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLE39_18845"
     CDS             <1..373
                     /locus_tag="PLE39_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNE70449.1"
                     /translation="YAVPGVYFYDPGVIEMAANLQPSKRGELEITDLNRLYLERGDLR
                     VEALGRGVAWLDAGTHESLLQASNFIQAVQERQGMMISCPEEIAYRMGFIDKKQLQEL
                     AGRLKNNSYGQYLFDLANGKI"
     gene            374..1240
                     /gene="rfbD"
                     /locus_tag="PLE39_18850"
     CDS             374..1240
                     /gene="rfbD"
                     /locus_tag="PLE39_18850"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNE70450.1"
                     /translation="MKILLLGNTGQLGWELQRCLPPLGDVRGLDYPQIDLANPETLRD
                     VIRTYKPDTIVNATAYTAVDVAESKSELAFAINAKATAVMAEEAKLSNAVMVHYSTDY
                     VFDGAKGSAYTEADATNPLNVYGASKLAGEQVVQAAGGAHLIFRTAWVYSLRRDSFVT
                     KTLQWARQHETLRLVDDQISNPTWARLLAEVTAQILARGPGYLCERTGLYHLAGDGYA
                     SRLEWARLILELDPNRHEQKVKEILPASTSDFPAPAMRPLFSALNCDRFTATFGLRLP
                     HWESALRMAMDR"
     gene            1353..1925
                     /locus_tag="PLE39_18855"
     CDS             1353..1925
                     /locus_tag="PLE39_18855"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE70451.1"
                     /translation="MNNEASHISQGSAPPETDIPNLVQRIQDGDSQAVAALYSMHYQQ
                     LYTFAYYRLEGNVPLAEDITEEAFLRAFKTIGTFKWKGVSFGAWLVTIARNLIVDMLR
                     RPDTLQLQETWLDATSDPEKLVEQTLTNKELGVALEKLTSEQKEVIILRFFDDFSIAE
                     TAKILGKSIDAIKRLQARALVVLESNLSRD"
     gene            1928..3313
                     /locus_tag="PLE39_18860"
     CDS             1928..3313
                     /locus_tag="PLE39_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70452.1"
                     /translation="MKTRNHTDDKLGEILEACLSDIKAGRATREECLSRYKDHARELE
                     PLLRVATSLSTHPKPRPSEKRVFTSRQRILQEFHSKERIPWFSGLRRSFTAPALAFST
                     VLTFIIMSFFGARVYFSTVTDEPTASAYPNLGPVITQTVPPQSEPTIAFNGIGLPGEQ
                     DGPYEIASAPDGTLWFARTDATRVGSVSAEDGKVQDWPLAANEGRAAGITVDKNGRVW
                     FTVSSSQSVIALDPATNEVSSWRVPPGDGTLGRIEAGEDGHIWFIARDGSRIYALDPK
                     TDNFTAYDVNGAEYIEWADDSLWFSGKGELGRIAPDGQVQVYDAPGDPAALVWDGSAL
                     WYAGGSLIGRFDAATDQVVVYPLENKTADRIATDGLKNIWFGSQGGTELGVLQSDSGQ
                     ISTRSMSAVVQNLYWLAVGTDQVWMSDGDSQFVYSFSLTKSGEVSFSVPINTQDQLAV
                     SIPLAALPAFH"
     gene            3674..>4215
                     /locus_tag="PLE39_18865"
     CDS             3674..>4215
                     /locus_tag="PLE39_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70453.1"
                     /translation="MNRKVFTLLLVAVFALAQFSVASAVQVTADGTLTAPDFMPAGAG
                     ATVTLDWTATGLPAGTTYIDIYARLQGGAYVVGECQNVAVIVGSNNSSGTFDYVFTGA
                     PADGEYWEFLIAFDDAATCTTLPALPADADNFMASSYVDGNKLDGYGFFPPFDGVVVT
                     TPVACNTFEYWAIASDNYNIP"
BASE COUNT          958 a         1112 c         1084 g         1061 t
ORIGIN      
        1 ctatgccgtg ccgggtgtat atttttacga ccccggtgtg atcgaaatgg cggcgaattt
       61 acagccctcg aaacgcggcg agctggagat caccgaccta aatcgtcttt acctcgaacg
      121 cggcgatttg cgcgtggagg cacttggccg cggggtggcc tggctcgatg ccggcaccca
      181 cgagtcgctg ctgcaagcct cgaacttcat tcaagccgtg caggaacgcc agggtatgat
      241 gatctcctgt cctgaagaaa tcgcctaccg catgggcttc atcgacaaaa aacaacttca
      301 ggaattggct ggcaggctca aaaacaactc ttatggtcaa taccttttcg atctcgccaa
      361 cggaaaaata tagatgaaaa tactactgct gggcaacact ggccaactgg gatgggaatt
      421 acaacgctgt ttaccgcctc tgggcgatgt acgcggtctg gactacccgc aaattgatct
      481 ggcaaatcca gagactctgc gcgatgtgat tcgcacctac aaaccggata ccattgtcaa
      541 tgccaccgcc tacaccgcgg tggatgtggc cgaaagcaaa agcgaactgg catttgcgat
      601 caacgcgaaa gccaccgcgg tcatggcgga agaagccaaa ctgtcgaacg cggtgatggt
      661 tcactattcc accgattatg tgttcgatgg agcaaaaggc tcagcctaca ccgaagccga
      721 tgccaccaat ccactgaacg tttacggagc cagcaaactg gcaggggagc aggtcgttca
      781 ggcggcaggc ggcgcgcatc tcattttccg tacagcctgg gtttatagcc tgcgccgtga
      841 cagttttgtc accaaaaccc tgcaatgggc ccgccaacat gaaaccctgc ggctggtgga
      901 tgaccagatc agcaacccca cctgggcgcg cctgctggcc gaagtaaccg cacagatttt
      961 agcgcgtggt cccggttacc tctgtgagcg gacgggtctg tatcatctgg caggggatgg
     1021 gtatgccagc cgcctcgagt gggcccgtct gatccttgag cttgatccta accgccacga
     1081 acagaaggta aaggaaattt tgcctgcctc cacctctgat tttccagccc cggcaatgcg
     1141 tccgctgttc tcggcgttga actgtgaccg ctttaccgcc acttttggcc tgcgcctccc
     1201 gcactgggaa tctgccttgc gcatggccat ggaccggtaa attccgtgtt tttctccctc
     1261 ccactgctct ttggggcctt aattaacctt gcagttttgc aattttcgac caattttcga
     1321 tttgccggtc tcttttttcg atttcatacg ttatgaataa tgaagcatca catatttctc
     1381 agggttccgc accacctgaa actgacattc ccaacctggt acagcgtatc caggatggtg
     1441 attcacaggc tgtagcagct ctgtatagca tgcattacca gcaactctat acatttgcat
     1501 actatcgcct ggaagggaat gttccgcttg ctgaggatat caccgaggaa gcgtttttgc
     1561 gtgccttcaa gaccattggc accttcaagt ggaagggtgt ttcttttggg gcctggctgg
     1621 tcaccattgc ccgtaacctg atcgtagata tgttgcgccg cccagatacc cttcaattac
     1681 aggaaacctg gctggatgcc acctctgacc cggaaaagct tgttgagcag accctcacga
     1741 acaaagaact gggcgtagcg ctcgaaaagc tgacatccga acaaaaagaa gtgattattc
     1801 tgcgcttttt tgatgatttt agcatcgcag aaactgcaaa aatcttaggt aaatcgatcg
     1861 atgccattaa acgccttcag gcacgtgcat tggtcgtact ggaaagtaat ctgtcgagag
     1921 actaattatg aagacaagga atcacacaga cgataaactg ggcgagatcc tggaagcatg
     1981 cctgtctgac ataaaagcag gccgtgccac ccgggaggaa tgcctgtcac gctataagga
     2041 ccatgcccgc gagctggagc ctttattgcg cgttgccaca tccttgtcca cccaccccaa
     2101 gccgcgacct tctgaaaaac gtgtgtttac cagccgtcaa cgcatccttc aagaatttca
     2161 ttccaaggag cgtatcccct ggttttccgg gttgcgccgc tcttttaccg ctcccgctct
     2221 tgcgttttca acggtgctta ccttcatcat catgtcgttt tttggggcgc gggtttattt
     2281 ttcaaccgtt acggatgaac caactgccag tgcataccca aacctgggtc ctgtaatcac
     2341 tcaaacagtc cctccccaat ctgagcccac cattgccttc aatggtatcg gcctgcccgg
     2401 tgaacaggat ggcccttatg agatcgcaag tgcccctgat ggcaccttgt ggtttgcccg
     2461 tacagatgcc acccgtgtgg ggtctgtaag cgccgaagat ggcaaggtgc aggactggcc
     2521 cctcgccgcc aatgaaggcc gtgccgcagg gattaccgtt gataagaatg gcagagtttg
     2581 gttcacggtt tcctcctctc aatcggtcat tgcattggac ccggcgacaa atgaagtctc
     2641 ttcctggcgt gtgccgccgg gagacggcac cttgggcaga attgaagccg gagaagatgg
     2701 acatatttgg ttcatcgcca gagatggcag ccgcatttac gcgcttgatc caaagacaga
     2761 caatttcacc gcctatgatg ttaatggtgc cgaatatatc gaatgggcag atgactcgct
     2821 ttggttcagc ggcaagggcg aactgggacg gatcgcgcca gatggtcagg ttcaggtata
     2881 tgatgcccca ggtgaccccg cagcgctggt ttgggacggg tccgcgcttt ggtatgctgg
     2941 tggcagcctg attggccgtt tcgatgcggc taccgatcag gtggtcgtat atccgcttga
     3001 aaacaagacc gctgaccgaa ttgccacaga cggcttgaaa aacatctggt tcggttcaca
     3061 gggtggtacg gaattgggag tcttgcaatc agatagcgga cagatcagca cccgctccat
     3121 gagtgccgtg gttcagaatc tgtactggct tgccgtaggt actgaccagg tctggatgtc
     3181 tgatggcgac agtcagttcg tttattcctt cagcctgacg aaatccggtg aggtaagttt
     3241 ctctgtaccc atcaacaccc aggatcagct tgctgtttct atcccgcttg ctgcattgcc
     3301 ggcatttcat taacctccag cataaacatt cattttttaa tatttccccc gaacgactca
     3361 gttcggggga tttttttagc ttaaaatccc gggtttcctg cccaagggtg ggcaatttga
     3421 gggtgggcag agcagatgac aggtcaaatt tgaggcgtgg gggcaattta ggaattggtc
     3481 gggtttgccg gtctcttttt cggagttcat ccgtttaatt aatgtaacgt gatttttttt
     3541 attgcgttac tgaaagagag atggggtaat taagttgtat gacctttcct gaaaggagat
     3601 gatacattac ataaatgccc atacttaatg atttaatcaa taaatgattt ctcagatcga
     3661 taaggagaga tatatgaaca ggaaagtatt tactttgtta ctggttgccg tatttgcact
     3721 ggctcaattt agcgttgcat ccgcagtgca ggtaaccgct gatggcaccc tgaccgcccc
     3781 cgattttatg cctgcaggcg ctggtgccac cgttacattg gattggaccg ccacaggcct
     3841 gcctgctggt accacctata ttgacattta tgcccgttta cagggtggcg cgtacgtagt
     3901 aggtgaatgc cagaacgtgg ccgtgatcgt tggctccaac aatagcagcg gcacctttga
     3961 ttacgttttc actggcgccc ccgctgatgg cgaatattgg gagttcctca ttgcgtttga
     4021 cgatgcggcc acctgcacca ctttgcccgc ccttcctgct gatgctgata acttcatggc
     4081 atcctcttat gtggacggca acaagctcga tggctatgga ttcttccccc catttgatgg
     4141 tgtcgttgta accacgcccg tggcttgcaa tacctttgaa tactgggcca ttgcttctga
     4201 caattacaac atccc
//
LOCUS       DAOQJC010000218         4180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_12934_length_4180_cov_9.931636, whole genome shotgun sequence.
ACCESSION   DAOQJC010000218 DAOQJC010000000
VERSION     DAOQJC010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12934_length_4180_cov_9.931636"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..2204
                     /locus_tag="PLE39_18870"
     CDS             264..2204
                     /locus_tag="PLE39_18870"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE70454.1"
                     /translation="MPSSKANVKLFLFFLFLFIMACGFSSATPEPTAIPANTEIPLPT
                     ATPIPSSLTVCLGQEPNTLYPYGGPNTAARSVLAAVYDGPIDMVGYEYQPVILSQLPS
                     IENGDAQIVPTTVQPGGQAVDAEGNLVLLERGKKFRPAGCRGDDCIITYDGSTPLEMD
                     QMVVTFRLREGLTWADGTPMVSDDSVYAYDISRDSNAVANTYLIDRTYTYEATDPLTV
                     QWWGMPGFIDPTYFTNFWTPAPRHLWSQFPADQLPEIDIASRTPVGWGPYVIQEWIAG
                     DHITLTKNPYYSAAADGFPKFDTLSFRFIADPDTALSEMVAGRCDILDPSIRLDNHAS
                     LLLEMEKGEQAQVFFTPGMTIEWLGIGTTPSTYDDGYDTTYQNDRPDFFSDPHTRQGI
                     AYCLDRQSVVNNVLFGLTTIPATYLPVGHPLYDNNLQPLPFDVATGVSLLEQAGWKDT
                     DGDPTTPRQAVNVKNVKAGTPLQLNYYTTTATQRRQVVEILQNSLAQCGIGLNVQYFS
                     QNDLYASGPDGALFGRRFDLIEYAMGVGSIEPPCDWFTSEEIPTSENSWVGTNVTGYR
                     NEAYDTACRAAQLSLPDEQTYFDSYRQTQVLFSSELPAIPLYYRLRVAAARPGLCHFE
                     LDPTANPLWNIEAIATGEACQN"
     gene            2204..4123
                     /locus_tag="PLE39_18875"
     CDS             2204..4123
                     /locus_tag="PLE39_18875"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE70455.1"
                     /translation="MRRLFHIGLFLTLLLVSCSPQPARVAEPTSIPTPTLAPVVAHAP
                     EIRFALVGEPQTVNVWQLFDTPGASYADYALRADSWPRLYHLLPPEFTFEPLAASGLP
                     TAFTQENDFYVAAVKLRTDLKWTDGSAFTAEDVAFTVNAALAFELGFDWSEFYPSDDL
                     HHAEALDASTVKYYFKQEPNVGAWQYGALQGPVVQKAFWEGAVNAAANSLPTETLRTE
                     IAKTQADLETVKRDVAELSEQVLALILSGKQNRILDSDLKRKQNEQIFVQSNLDRLLE
                     EYAANIAAAQSALYASSDEKEPLLGSWIPLGMEDGQWVNEANLEFPFIQPGFDRAVYK
                     IFESETEALTAFENGEVDAILAPAGISSNIPGMVYSSTYSARFLVFNPLKMQFADPAF
                     HSALSCMLDRNTLSSDRLQNQAFPFDAFVLSPQWQDSAAQDPCTGLDEATRIGQAVKF
                     MKDAGYFWAKEPDANGAGQGLRYSNGDLFPTAVLLSPTKDEDPLRYASANYVSERAEY
                     LGVSITVQTASMDDLIYAVYSSQQYDLAITGWRLSEYPAYLCEMFDGRSQHLYTSDRF
                     QSACDALKGESDLERARQGFRQIESALMMELPFIPLFTVARADLYQKIAYPAEKVLNS
                     WGGLYGAPSFAVPLP"
BASE COUNT          972 a         1172 c         1000 g         1036 t
ORIGIN      
        1 tctacaagga agcgaaccag ctttactacg atctggctgt tggtattccg ttggaagttg
       61 cgacctccca cggtttctca cagcgctggg tacagggtat tgtattcaac ccgatcttcc
      121 ccggcttcta ttactacacc atgtacaaag actagtcttc tgactggttg atgtagactt
      181 actatgcaaa aaaagcaagc cgcccgacaa aggcggcttg cttgtataat tggcagatgc
      241 gttttttcaa aaaggaattt gccatgccct cttccaaagc gaatgtaaaa ctgttcctct
      301 tttttctctt tctatttatc atggcctgtg gtttttcctc tgccacgccg gaacctacag
      361 ccattcctgc caacacagaa attccccttc ccacagcaac cccgatcccc agttcgttga
      421 cagtatgcct gggtcaggaa cccaatacct tgtatcccta tggcggaccg aacaccgcgg
      481 cccgcagtgt tttggccgcc gtttacgacg ggccgattga catggtcgga tatgagtatc
      541 agccggtcat tctttcgcaa ttgccgtcta ttgaaaacgg cgacgcccag atcgtcccaa
      601 ccacggttca gcctggaggt caggcagtgg atgcggaagg gaatctcgtc ttgctggaac
      661 gcggtaaaaa attccgccct gctggctgcc gcggcgatga ctgcatcatc acctatgatg
      721 gctccacccc gctcgaaatg gaccagatgg ttgtcacctt ccgcttgcgc gaaggattga
      781 cctgggcaga cggcactccc atggtttccg atgattcggt gtatgcctac gatatttcac
      841 gcgattccaa cgcggttgca aatacctatc tcattgaccg tacctatacc tatgaagcca
      901 ctgacccgct caccgtgcaa tggtggggca tgccaggctt cattgaccca acctacttta
      961 ctaatttctg gacacccgct ccacgccatc tgtggagtca gtttccagct gaccaattac
     1021 ccgaaattga tatcgcctca cggacaccag tcggttgggg accatacgtc attcaagaat
     1081 ggatcgcagg ggaccatatc accctgacaa agaatcctta ttattcagcc gccgcagatg
     1141 gttttccgaa attcgatacc ctgtcattcc gttttattgc tgaccccgat accgcgctga
     1201 gcgaaatggt ggctggccgt tgtgacatcc ttgatccaag catccgactc gataaccatg
     1261 ccagtctgtt gctggaaatg gaaaaaggcg aacaggcaca ggttttcttt acgcccggca
     1321 tgaccattga atggctgggc attggcacaa ccccatccac ctacgatgat gggtacgaca
     1381 ccacctacca aaacgaccgc cccgactttt tctccgaccc gcacactcgt cagggcattg
     1441 cttattgtct cgaccgccaa tccgttgtca acaatgttct gttcggcctc accaccatcc
     1501 cagccaccta tcttcccgtt gggcatccgc tgtatgacaa caacctgcaa ccgcttccat
     1561 ttgatgttgc cacaggtgtt tcgttgcttg aacaggcagg ctggaaggat accgacggcg
     1621 atcccaccac cccgcgtcag gcggtcaatg taaagaacgt gaaggcaggc acaccccttc
     1681 aactgaatta ctacaccacc acagccaccc aacgcaggca ggtggtggaa atcctccaaa
     1741 attcactggc gcagtgcgga atcggactca acgtgcaata cttctcgcaa aatgatttgt
     1801 atgcctccgg acccgatggg gcactctttg gccgccgttt cgaccttatt gaatatgcca
     1861 tgggtgtagg cagcattgaa cctccttgcg attggttcac ctccgaagaa attccaacca
     1921 gcgaaaactc atgggtcggc accaacgtta ccggttaccg caatgaagcc tacgatactg
     1981 cttgccgcgc cgcacaattg tccctgcctg atgagcaaac ctactttgat tcgtaccgtc
     2041 aaactcaggt gttattctct tccgaactcc ctgccatacc gttgtattac cgcctgcgag
     2101 tggccgctgc ccgccccggc ctttgtcact tcgaactcga cccgaccgcc aacccgcttt
     2161 ggaatatcga agcgatagcc accggcgaag cctgccagaa ttaatgcgac gactcttcca
     2221 cattgggctt tttctaaccc ttttgctcgt ctcatgctcc ccgcaacctg cccgggtggc
     2281 agagccaacc agcattccca ccccgacgct tgcccctgtt gtggcgcatg cacctgagat
     2341 tcgatttgca ttggttggcg aaccccaaac ggtcaatgtt tggcaattgt tcgacacccc
     2401 cggcgcatcc tatgcagact atgctttgcg cgccgactct tggccgcgcc tctatcacct
     2461 cctgcctcca gaatttactt tcgaacccct ggcagcatct ggcttgccca ccgcattcac
     2521 gcaggaaaac gatttttacg tcgctgccgt caaactccgt accgatttga agtggaccga
     2581 tgggtctgca ttcactgcag aagatgtcgc cttcacggtg aatgccgccc tcgctttcga
     2641 actcggtttt gactggagcg aattctatcc gagtgatgat ctccaccatg ccgaagcgct
     2701 ggatgcatcc acggtgaaat attatttcaa gcaggaaccc aatgtaggcg catggcaata
     2761 cggcgcattg cagggccctg ttgtgcaaaa agctttttgg gaaggggcgg taaatgccgc
     2821 ggccaattcc ctgccaaccg aaaccctgcg aaccgaaatc gcaaagacac aagccgacct
     2881 tgaaacggtc aaacgggatg tggccgagtt gagcgaacag gtgcttgcat tgatcctgag
     2941 cggcaaacaa aatcgaattt tagacagtga cctcaaacga aagcagaacg aacagatctt
     3001 tgtgcaaagc aatctcgacc gcttgcttga agaatatgcg gcaaatatcg ctgcggccca
     3061 atctgctttg tatgcttctt ctgatgaaaa agaacccctg cttggcagtt ggattcctct
     3121 cggcatggaa gatggtcaat gggtgaatga agccaacctt gagtttccat ttatccagcc
     3181 gggttttgat cgtgccgtgt ataaaatttt cgaaagcgag accgaagccc tgaccgcttt
     3241 tgaaaatggg gaagttgatg ccatcctcgc acccgctgga ataagttcga acatccccgg
     3301 aatggtgtat agctccacgt atagtgcgcg tttccttgtg ttcaatccct tgaaaatgca
     3361 atttgccgac cctgcctttc attctgccct cagctgtatg ctggatcgaa atacactttc
     3421 gtctgataga ctgcaaaacc aggcctttcc attcgatgct tttgtgcttt ctccgcaatg
     3481 gcaggattct gccgcacaag atccctgcac cgggttggat gaagccacac ggatcgggca
     3541 ggcagtgaaa tttatgaagg atgccggtta tttttgggcg aaagagcctg atgcaaacgg
     3601 cgccgggcag ggactgcgct attcaaacgg tgatttattt ccaacagcgg tcttactgag
     3661 tcccacaaaa gacgaagacc cattgcggta tgcctctgct aattacgtgt cagaacgagc
     3721 tgaatatttg ggcgtttcca tcacagtaca gacagcatcc atggatgatt tgatctatgc
     3781 tgtgtacagt tctcaacaat atgacctcgc cataacaggc tggcggctga gtgaataccc
     3841 ggcttatttg tgcgaaatgt tcgatgggcg gtcacagcat ttgtatacca gtgacaggtt
     3901 ccagtccgcg tgtgatgcgc taaaaggaga atctgacctg gagcgggcgc ggcaggggtt
     3961 ccgtcagatc gagtctgctt tgatgatgga actgcccttc attccgcttt ttacggtggc
     4021 gcgggctgat ctgtatcaaa aaatcgccta tcctgccgaa aaagtcttga atagttgggg
     4081 cggattgtat ggagcacctt cctttgcagt tccattgcca tagttaggtg gcgcaattcg
     4141 catttttgtt gttggaagtt ataattgttt gagaaagaag
//
LOCUS       DAOQJC010000219         4170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_12973_length_4170_cov_9.867558, whole genome shotgun sequence.
ACCESSION   DAOQJC010000219 DAOQJC010000000
VERSION     DAOQJC010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12973_length_4170_cov_9.867558"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /gene="miaB"
                     /locus_tag="PLE39_18880"
     CDS             <1..1362
                     /gene="miaB"
                     /locus_tag="PLE39_18880"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNE70456.1"
                     /translation="MKYHIWTEGCQMNVADSQRVGSSLEHLGYTFTETIEEADVIVLN
                     TCVVRQSAEDKALGRLSSLAPLKKKNPNLVINLMGCLVGVRGTEKLKARLPYVDVFSP
                     PSDPGPLISHLTQGEIRSMEDAETTRRFLMMDDELILPVAEQGKLVTAHVPIVYGCSH
                     ACTFCIIPYKRGVERSRPVGDIVSEVRSLAKQGVKEITLLGQIVDRYGKDIPDGPNLA
                     ALLRIIHDEPGAEGIERIRFLTSHPNYFDDDLIQAIAELPRVMPHIELPIQAGDDEVL
                     ANMKRGYTQQNFRDLVEKIRAHIPDCSIATDIIVGFPGETDEQFMETYRVLSDLKLDV
                     AHLARYSVREGTVATRRMEDNVSDEEKRRRFHLLEELQEQVVGEINKKYLGETVDVLF
                     EEKVKGRWRGRTPTNKLVFVESEEDLRGKLRPVTVTWTGPWSMQAQLAGVRSQNKPEL
                     ISL"
     gene            1424..2341
                     /locus_tag="PLE39_18885"
     CDS             1424..2341
                     /locus_tag="PLE39_18885"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNE70457.1"
                     /translation="MSFNELITTFANNLLPILLVSGAGFILGKFLTVDSRSLGRVVFY
                     VFSPLLVLDLMLKSQLNLQQAMTTVGYTMSVIAIMGLLAFLFGKLFKLEKPYLLAVIL
                     TVAFGNTGNYGLPLVKFAFGTDALAVASLFYVTTTILFNTVGVVIASIGHMDLKSAVL
                     GLFKLPSIYGVTLALIIKGLGIQLPLPISRTIEIAANGAIPVMLILLGLELTRIEWSH
                     SFRALGLGVAVKLLLGPLVGLALAGLFKLQGHVRQGAVLEAAMPAAVATTVVATEYKL
                     EPSLVTAIVFLGTALSPLTLTPLIVFLTR"
     gene            2344..3570
                     /locus_tag="PLE39_18890"
     CDS             2344..3570
                     /locus_tag="PLE39_18890"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HNE70458.1"
                     /translation="MPHLKKLIARLMAILLTAAILPFNLNQAGAQGGIDVTAATAVVD
                     FGRTITFQATITAPIGIQQVSLLFRGMNEDVTRVEPVQLGPDGFVSFTYDASRNFFPP
                     FSSVVFWFQATLADNQTYTSQPITFAYNDNRFPWREARRDNLVVHWYAGDDAFGAAAL
                     DAANRGILSINEFIPISSSQPIDIYIYSNALDLQNTLLLGGQEWVGGHANPELGVVLV
                     AISPGVNQSIELETMIPHELAHVMLYRSLGGTYNIQPLWLLEGIATMAEYPNPDYKHA
                     LDIASGNNTLLSFADLCVSFPADAGNAYLAYAQSQSFVTYIRETYGTSGLARLTKSYS
                     EGFNCELGATNALGTPLSLLDVRWRESVLGQNVAGVAFRNLSPYLLLMALVLVVPMWG
                     AIDMLRQRRKRAKQSD"
     gene            3551..3853
                     /locus_tag="PLE39_18895"
     CDS             3551..3853
                     /locus_tag="PLE39_18895"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNE70459.1"
                     /translation="MQNNPTDELKRVHMWVKGRVQGVGFRAHVEYHALQIGVLGWVRN
                     LGHDTVETVAEGTKEQIAQFIEMVKTGPRHSRVDEARVEYEPTTGHLSGFTVKMSM"
     gene            complement(3856..>4170)
                     /locus_tag="PLE39_18900"
     CDS             complement(3856..>4170)
                     /locus_tag="PLE39_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HNE70460.1"
                     /translation="EHLRVLLLDRRNRVLETVEVYKGSVNSSQVRVGELFKDAIRANA
                     SAIIVAHNHPSGDPTPSPDDVAVTRAMVQAGKLLDVDVLDHLVIGLGRWVSLKERGLG
                     FS"
BASE COUNT          971 a         1179 c         1034 g          986 t
ORIGIN      
        1 atgaaatatc acatctggac cgaaggatgc caaatgaacg ttgccgactc tcagcgggtt
       61 ggctcctcgc ttgagcatct gggttacacc tttaccgaaa ccatcgaaga agccgacgtt
      121 attgttttga acacctgcgt tgtgcgccaa tctgccgaag acaaggcgct tggccgctta
      181 tcatcgctgg ccccgctcaa aaagaaaaac cccaacctcg ttatcaattt aatgggctgc
      241 ctcgttggtg tgcgcggcac cgaaaaatta aaagcccgcc tgccctatgt ggatgtcttc
      301 tccccaccct ctgacccggg cccattgatc tcgcacctca cccagggaga aatccgctcg
      361 atggaagatg cggaaacaac ccgccgattc ctgatgatgg acgatgaact gatcctcccc
      421 gtggcagaac agggcaaact cgtcactgcc catgtgccga tcgtttacgg atgttcacat
      481 gcctgcacct tttgcatcat cccctacaag cgcggcgtgg aacgctcccg ccctgtcggc
      541 gacattgtca gcgaagtccg ctcactggca aagcaaggtg taaaagaaat tacattgctc
      601 ggtcaaatcg tagatcgcta cggtaaagac atccccgatg gtcccaacct cgccgcgttg
      661 ctccgcatca ttcacgacga gccaggcgcg gaaggcatcg aacgcatccg cttcctgacc
      721 tcgcacccca actattttga cgacgatctc attcaagcca tcgctgaact cccgcgcgtc
      781 atgccgcaca ttgaactccc cattcaggca ggcgatgatg aagtgctcgc caacatgaaa
      841 cgcggataca cccagcaaaa cttccgcgac ctcgtggaaa aaatccgcgc ccacattccc
      901 gattgttcca tcgccaccga catcatcgtc ggattccccg gcgaaaccga tgaacaattc
      961 atggaaacct atcgcgtctt atcggacctg aaactggatg tggcgcatct ggcacgctac
     1021 tccgttcgag agggtaccgt cgccacccgc cgcatggaag acaatgtaag cgacgaagaa
     1081 aaaagacgcc gcttccacct gctcgaagaa ttgcaggaac aggtcgtcgg cgagatcaac
     1141 aagaaatatc tcggcgaaac cgtggatgta ctcttcgaag aaaaggtcaa aggccgctgg
     1201 cgcggacgca cccccaccaa caaactggtc ttcgtcgaat cagaagaaga cctgcgcggc
     1261 aaactgcgcc ccgtcaccgt cacatggacg ggaccctggt ccatgcaggc gcagctcgct
     1321 ggggtacgct cgcaaaataa accagaattg atttcgctat aaaaatgtac ggacacggcg
     1381 ttgccgtgtc cgtatgattt tcgggggtta taatttcgcc accatgtctt tcaacgaact
     1441 cattactaca tttgcaaaca acctgctccc catcctgttg gtcagtggag cggggtttat
     1501 tctcggaaaa ttcctcactg tcgattcgcg ctctcttggt cgggtggtct tttacgtatt
     1561 cagccctctg ctcgttctgg atctgatgct caaaagccag ctaaatctgc aacaagccat
     1621 gacgaccgtg gggtacacca tgtcggttat tgccataatg ggtttgctgg ccttcttatt
     1681 cggcaaactc tttaaactgg aaaagcccta tctgctggcg gtcattctta cagtggcctt
     1741 cggcaacaca ggcaattacg gcctgccgct ggtgaagttt gccttcggca ccgatgcatt
     1801 ggcggtcgct tccctgtttt atgtgaccac caccattttg ttcaatacgg tgggtgtggt
     1861 gatcgcttcc atcggccaca tggatctgaa atcggcagtg ctggggttgt tcaaacttcc
     1921 cagtatctac ggtgtgacac tggccctcat aataaaaggg ttgggaattc aactccctct
     1981 gcccatttca cgtacaattg agatagccgc aaacggagcc attcccgtca tgcttatttt
     2041 gctcggcttg gaattgaccc gcatcgaatg gtcacacagc ttccgcgcgc tggggttggg
     2101 cgtggcggtg aaacttttac ttggaccgct ggttgggctt gcattggcag ggttgttcaa
     2161 attacagggg catgtgcgtc agggtgccgt attggaagcc gccatgcctg ccgccgttgc
     2221 aaccacggtt gtggcaaccg aatataaatt ggagccatcg ctggtcactg caattgtttt
     2281 tctaggcaca gcgctcagtc cgctgacatt gacgccattg attgtttttc taacgaggta
     2341 aacatgccgc atctaaaaaa actcatcgcc cgattgatgg cgattctact tacagccgcg
     2401 atcctgcctt ttaacttgaa tcaagccggg gcgcagggag gaatcgatgt gactgctgcc
     2461 acggcggtgg tggattttgg tagaacgatc acatttcagg caacgatcac agctccgatc
     2521 ggcattcaac aggtttctct tctcttccgc ggtatgaatg aagatgtgac tcgggtggag
     2581 cccgtgcagc ttgggcctga tggatttgtg agtttcactt acgatgcctc gcgcaatttt
     2641 ttcccgccgt ttagttcggt cgtattctgg tttcaagcca ctttggccga taaccaaacc
     2701 tacaccagcc agcccatcac ctttgcttac aacgataatc gttttccatg gcgtgaggca
     2761 aggagagata accttgtggt gcattggtat gcgggcgatg atgcttttgg cgccgccgca
     2821 ttggatgccg ccaacagggg catcctttcc atcaatgagt tcattcccat ttcttcaagc
     2881 cagcccatcg atatttacat ctattcaaat gcactcgacc tgcaaaatac gctcttgctt
     2941 ggcggccagg aatgggtcgg cggacatgcc aacccggagt tgggcgtggt gctggttgcc
     3001 atttcccccg gcgtcaatca atccattgaa cttgaaacca tgatcccgca cgaacttgcg
     3061 catgtgatgt tgtaccgttc gctgggaggg acttacaaca ttcaaccgct ctggctgctt
     3121 gaaggcatcg ccaccatggc ggaatatccc aaccctgatt acaaacatgc gctggatatt
     3181 gccagcggaa ataatacctt gctctctttt gcagacttat gcgtctcttt ccccgccgac
     3241 gcaggcaacg cctatctggc ttatgctcaa tcccaatcat ttgtcaccta catccgcgaa
     3301 acctacggca cctcaggcct ggcacgtttg accaaatcat acagcgaagg ctttaactgc
     3361 gaactcggtg caaccaacgc ccttggcacc ccattgagcc tgctggatgt gcgctggcgc
     3421 gaaagtgtgc ttggacaaaa cgtcgcaggt gtggcttttc ggaatctttc accttatctg
     3481 ttgttgatgg ctctggtgtt ggtcgttccc atgtggggcg cgattgatat gctgcgccaa
     3541 aggagaaaac gtgcaaaaca atccgactga cgaattgaaa cgcgtacaca tgtgggtcaa
     3601 gggacgggtg caaggggtgg gcttccgtgc gcatgtggag taccatgccc tgcagatcgg
     3661 tgtgcttggc tgggtgagaa acctcggtca cgatacggtt gaaaccgtgg cagaaggcac
     3721 caaagaacag attgctcaat ttatcgagat ggtgaaaaca ggtccgcggc actcacgagt
     3781 ggacgaagct cgcgtggaat atgaacccac caccggacat ctaagcggtt tcaccgtaaa
     3841 gatgagcatg taacatcacg aaaaccccaa ccctctttcc ttcaacgaaa cccatctccc
     3901 cagtccgatc accaaatgat cgagcacatc cacatccaac agctttcctg cctgcaccat
     3961 ggcgcgtgtc acggctacat catcggggct gggagtcgga tcgccgcttg ggtggttatg
     4021 cgcaactata atcgcggatg cattggctcg aatcgcatct ttgaacaact cccccacacg
     4081 aacctgtgat gaattgaccg agcctttata aacttccaca gtttccaaga cccgattcct
     4141 tctgtccaac aaaagcaccc gcagatgttc
//
LOCUS       DAOQJC010000220         4167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_12989_length_4167_cov_11.335360, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000220 DAOQJC010000000
VERSION     DAOQJC010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12989_length_4167_cov_11.335360"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1484
                     /locus_tag="PLE39_18905"
     CDS             99..1484
                     /locus_tag="PLE39_18905"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNE70461.1"
                     /translation="MSAETKPAVIGFLPDDTPPIGAMLSLGFQQFLTMFPATVLVAIL
                     TKFDVGITLLASGLGTIIALLVSKRKIPMYYGSSFSYIAVIITVMNKFAPDCYNSAAT
                     YCPDGVRIVQVGIILTAVFEILIGLLIMRIGKAALDRVLPPIVTGSMAMVIGIALANA
                     ALGNAGNWAAPELAATTWTVAFITLIATVLFSVYLQGKGMIGMLPILFGAIFGYLVAI
                     PFGLVHFENVASASWLAMPKFTLPAFENPEAWGMALSIALIFIATVPESTAHLYQMSL
                     YIDELAAEMKRKPYNIKELIGLNLVADGSDDIVVGLLGGCAGTNYGENNSLMAITRNY
                     STAVLMTAGAMAMVFGFFGKLVALVNTMPAAVVGGLSIYLFGVIGMQGVALIQSEKVN
                     LFDPKQLAVGAIILICGIGGNLALPNGVFPFNVPFLFPNGIPAIVFAAIVGIFINTIF
                     LIFKTPEVRGG"
     gene            1572..2648
                     /locus_tag="PLE39_18910"
     CDS             1572..2648
                     /locus_tag="PLE39_18910"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE70462.1"
                     /translation="MIKLPGLIDPHVHLREPGATHKEDFDSGTSAALAGGVTIVLAMP
                     NTKPPIFDAATLDLALDAARQKARCDYGQYVGAGPDNSDWISHKAESDRAAGLKMYLD
                     STFGELRLDDMSLWTPHFANWPKEMPIVAHSESRSMAAAILFAAIHDRPVHIAHISLK
                     EEVLLIKAAKEHGLKVTCEVCPHHLFLSKDDIPAISHGHPGRGEVRPRLATKADVEAL
                     WANMDVIDCFATDHAPHTIAEKDSDNPPPGFPGLETFLPLILTAVQDGRLTMDDVISK
                     MYTNTKHIFNLPEQPETWVEVDENAQYEIKAAEQFTRCHWTPFEGWKVKGRVRKVVLR
                     GKTAFEDGKILAEPGYGKNIRSFN"
     gene            2865..>4167
                     /locus_tag="PLE39_18915"
     CDS             2865..>4167
                     /locus_tag="PLE39_18915"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNE70463.1"
                     /translation="MNKKAFIVLLLAAIVLAQFSLASAAAVSGDASITSVPTFLPLKQ
                     NLDVEWAVSGLPAGGPRYLLMWARREPSTTYDMAECKVVENLTGLTEASGAFEYSFAG
                     TNIAFLMIDGDVIETTLTLDTDDCAGTPDLPADSSVMGSTTIDNKVPSMRAGNPPSLS
                     AANIMLQPVACNTFEMWAVASDIAGLDPDAPYSGLVSWGQTTNGTFTPAAPIGKAEDT
                     LSWVYTFPATATGQWTFYIHPKDAAGNSGLLGNIYRLTLPIEPQELEDCAIFNDVSGH
                     KYEVYIRYLATLGLINGFADGSFGPDNTLTRAEAATLFEISNGYDENTLPASAPAGCE
                     FSDVTASDWFAGWVWQACADGFMNGVGGGLFDPNNLLTRGQVVTIMNNVANSTPAGGY
                     LNTNSIVNNLWNHFIIKPVLKAAFTDVPVGAYYADPVIQAYG"
BASE COUNT          949 a         1149 c          979 g         1090 t
ORIGIN      
        1 catcactttt tcggaacaga tgtgttttta tttttgtgaa cgagagagtc gcttgagcgg
       61 ctctcttttt ttatatccac acacccaaag gagagaatat gtccgcagaa acaaaacccg
      121 ccgtcatcgg tttcctaccc gatgacacgc cgcccatcgg cgcaatgctc agccttggtt
      181 tccagcaatt cctcacaatg tttcccgcca ctgtcctggt ggctattctg accaagttcg
      241 atgtcggcat taccttgctg gcctcgggtc ttggaaccat cattgccctg ctggtttcaa
      301 agcgtaagat ccccatgtac tacggctcat cgttctccta catcgcagtc atcattacag
      361 tgatgaacaa gttcgctccc gattgttaca actctgctgc cacatactgc ccggatggag
      421 tacgtattgt gcaggtgggc atcattctga ccgctgtatt tgaaatcctc attggcctgc
      481 tcattatgcg gatcggaaaa gccgctcttg atcgtgtgct ccctcccatc gtcacaggct
      541 caatggccat ggtgatcggt attgcattgg caaatgccgc gttgggcaat gccggtaact
      601 gggccgcccc cgaactcgcc gctacaacat ggactgttgc ttttattacg ctgatcgcca
      661 ccgttctttt ttctgtatac ctgcaaggca agggaatgat cgggatgctg ccgattcttt
      721 tcggcgcgat ctttggttat ctcgtggcca ttccattcgg tttggtccat ttcgagaatg
      781 ttgccagcgc cagttggctg gcaatgccca aattcaccct gcctgctttc gaaaacccgg
      841 aagcctgggg aatggctctt agcattgcat tgatcttcat tgccactgtt cccgaatcca
      901 cggcacatct ttaccagatg agcctgtaca ttgacgaact tgctgcggag atgaaacgca
      961 agccttataa catcaaggaa ctcatcggcc tcaatctcgt tgctgacgga tcggatgaca
     1021 tcgttgttgg cctgcttggc ggatgcgccg gtacaaacta cggtgaaaac aactcactga
     1081 tggctatcac ccgcaattat tccactgcag tattgatgac agcgggggcc atggccatgg
     1141 tctttggttt ctttggcaaa ctggtggcgt tggtcaacac catgcctgcc gctgtggtcg
     1201 gtggattgag catttatcta tttggcgtca ttggaatgca gggtgtggcg ctcattcaat
     1261 cggagaaggt caatctcttt gacccgaaac aacttgcggt cggcgccatt attttgatct
     1321 gcggcatcgg tggcaatctt gccctgccca atggcgtgtt cccattcaac gtgcctttcc
     1381 ttttcccgaa tggcatccct gcgattgttt tcgcggccat tgtcggcatt ttcatcaaca
     1441 ccattttcct gatcttcaag actccagaag tgcgtggagg ataggtctcg gaatgtcaac
     1501 caggcaaaca ggtacacatt tgtacctgtt tgcctatctc atcttcgcgt gttttccttt
     1561 atacttttct catgatcaaa ctccctggtt taattgaccc ccatgttcat ctgcgcgaac
     1621 ccggcgcaac ccacaaggaa gattttgatt cgggtacttc tgccgcgctg gctggcggtg
     1681 tgaccattgt gctggccatg ccaaatacaa aaccgcccat cttcgatgca gccacactgg
     1741 accttgctct ggacgccgcc cgtcaaaagg ctcgctgtga ttacggtcaa tatgtcggcg
     1801 ctgggcctga taactcggac tggatcagcc acaaggcgga gtctgaccgt gcggcgggtt
     1861 tgaaaatgta tcttgattca accttcggcg aattgcgtct cgatgacatg tcgctctgga
     1921 ctccgcactt tgcaaattgg ccaaaggaaa tgccgattgt ggcgcattca gagagccgca
     1981 gtatggccgc cgcgattcta ttcgccgcga tccatgaccg ccccgtacat attgcgcaca
     2041 tttcgttaaa agaggaagtt ctgctcatca aggctgccaa ggaacacggc ctcaaagtga
     2101 cctgcgaagt ctgcccgcat cacctgttcc tttcgaagga tgatatcccc gccatttcgc
     2161 atgggcaccc cggtcgtgga gaagtgcgtc cgcgccttgc tacaaaagca gatgtggaag
     2221 ccctgtgggc aaatatggat gtaattgatt gctttgcaac cgatcatgcc ccgcatacca
     2281 ttgctgaaaa agactcagat aacccgccgc ccggctttcc cggactagag acctttcttc
     2341 ctttaatact caccgccgta caggatggcc gcttaacaat ggatgatgtt atttcaaaga
     2401 tgtatacaaa cacaaaacac atcttcaacc tgcctgaaca gcccgaaacc tgggtggaag
     2461 tggatgagaa tgcccaatat gagatcaagg cggctgaaca gtttacccgc tgccattgga
     2521 ctccttttga gggttggaag gtgaaaggcc gtgttcgtaa agttgttttg cgtggaaaaa
     2581 cggcctttga agatgggaag atcctggctg agcctggtta cggaaaaaat attcgatcct
     2641 tcaattgaac cgaatcataa tgccacacta gagcggatcc tttaacgggt ccgctctttt
     2701 tattacctta ttgttatttt cagactgact ttttattcaa agaccctagt accaatatca
     2761 catccctttc ccctctattt agagaggtta caaactttcg atccgtttat agaatttcag
     2821 tcacaatcaa agaattgtaa tcatcataat aaaggagtaa gtatatgaat aagaaagctt
     2881 ttatcgtttt gcttttggca gccattgtgc tggcccaatt cagcctggct tccgccgctg
     2941 ctgtcagcgg ggatgcatcc attaccagtg ttcccacctt ccttccactt aaacagaacc
     3001 ttgatgtgga atgggctgtc agcggcctgc ctgcaggggg accccgctac cttttaatgt
     3061 gggcacgcag ggaaccatca acaacttacg acatggctga atgtaaagtg gttgaaaacc
     3121 ttaccggtct tactgaagcc agtggagctt ttgaatatag ctttgccggt accaacatcg
     3181 ctttcttaat gatcgatggc gacgtcatcg aaaccaccct gacccttgac accgacgatt
     3241 gcgcgggcac gccagacctg ccagcagata gttcagtcat gggcagcacc accatcgaca
     3301 acaaagtccc atcgatgagg gcaggcaacc cgccctcact ttccgctgcc aacatcatgc
     3361 ttcaacctgt tgcttgtaac acctttgaaa tgtgggcggt tgcctctgat attgcaggcc
     3421 ttgatcccga tgcaccatac tcagggcttg tttcctgggg gcaaacaacc aacggcacct
     3481 ttacccccgc cgccccaatt ggcaaggctg aagataccct ttcgtgggta tacaccttcc
     3541 cagccacagc taccggccaa tggacgtttt acatccaccc gaaagatgcc gccggaaaca
     3601 gtggcctttt agggaacatc taccgtctca cactccccat cgaaccgcag gaattggaag
     3661 actgtgccat cttcaatgac gtcagcggac ataaatatga agtgtacatc cgctatctgg
     3721 ccacccttgg cctgatcaac ggcttcgccg atggaagctt cggccccgat aacaccctca
     3781 cccgtgctga agccgccacc cttttcgaaa tctccaacgg ctacgatgaa aacaccctgc
     3841 ccgcttctgc cccggctggt tgtgaattct ccgatgtcac ggcttctgat tggttcgctg
     3901 gctgggtctg gcaggcctgt gcagacggct tcatgaacgg tgttgggggt ggactcttcg
     3961 atcccaacaa cctgctcacc cgcggacaag tcgttaccat catgaacaat gtcgccaatt
     4021 ccacacctgc cggtggctat ttgaatacaa acagcattgt gaacaacctt tggaaccatt
     4081 tcattattaa gccggttctc aaggcagctt ttacagatgt ccctgtcggc gcatattatg
     4141 ccgatcccgt gattcaagcc tacggtt
//
LOCUS       DAOQJC010000221         4130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_13164_length_4130_cov_9.529571, whole genome shotgun sequence.
ACCESSION   DAOQJC010000221 DAOQJC010000000
VERSION     DAOQJC010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13164_length_4130_cov_9.529571"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..744
                     /locus_tag="PLE39_18920"
     CDS             250..744
                     /locus_tag="PLE39_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNE70464.1"
                     /translation="MRKSTLFISAMLTVFLMATLFGVISAYQNIMATNQAVVVTPAEL
                     SVQEPAATAVPTIAAPMVISPEQATAIAVELLGDSNVYSVEVVDYEGVAAYLVTFSAG
                     DLVYVSSTGEVLAVTKLEPVVMVVPAGNRNDSGEKPNRDQQQQEDDGDDDDHGEDEHE
                     DGDD"
     gene            759..1088
                     /locus_tag="PLE39_18925"
     CDS             759..1088
                     /locus_tag="PLE39_18925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70465.1"
                     /translation="MNPKPAPRKWTDIQLAIAAVSMTSVLAFWNMFAGPDRSKSEEKA
                     AEAQQASQAAPTSAVMSVSPAPTMPPAGYTILFGGTAPQPQVIVMQSGQGGNQGGGDP
                     VTVTTSS"
     gene            1091..2008
                     /locus_tag="PLE39_18930"
     CDS             1091..2008
                     /locus_tag="PLE39_18930"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNE70466.1"
                     /translation="MLHRFSFRAMGSDILVILEQDSEAKPAILKDIPAWFENWEQTLS
                     RFRPTSELSHLNRTFDQPIKVSDAFWDVFQTALWADEISGGLVTPTVLDSMVEAGYDR
                     TFDKLPRSQFDMIASPAEADHPLSTVISDCDAKTINLPKGTGLDFGGVAKGWAAHQAV
                     ERLQEFGPCLVNAGGDIAVSGPRMDGSPWPVGVSNPFNVGANINVLQVKQGGIATSGK
                     DRRNWNRNGIFYHHIINPLTGLPAETDLLRVTVVAPTTMQAEAAAKTAFILGQEKGLH
                     WIEAQPEFAGLLVLETGDVIHSRRMQKYL"
     gene            2023..2757
                     /locus_tag="PLE39_18935"
     CDS             2023..2757
                     /locus_tag="PLE39_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70467.1"
                     /translation="MNAQPQSEFNESSVNIQSFLLFLFATVIGLLVAVLLLPIFLPNM
                     AFSLSGDSPKAYWYLSRATAFVSLSLLWLSMALGLGITNKMARLWPGMPATFAIHEYV
                     SLLGLAFAIFHALVILGDHYINFTLLQLFIPFSTVEYRPFWVGGGQIGFYIWAIVALS
                     FYIRPVIGQKFWRFLHYASFGMYGMGILHGIFSGTDTSLAWAQNYYWYSAGSLIFLFF
                     VRIVGSLMDVLFPAKKQTPAARPTQG"
     gene            complement(2838..>4130)
                     /gene="dnaK"
                     /locus_tag="PLE39_18940"
     CDS             complement(2838..>4130)
                     /gene="dnaK"
                     /locus_tag="PLE39_18940"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNE70468.1"
                     /translation="VSILEVGEGVIEVKATNGDTHLGGDDWDQKITNWAADEFKKDQG
                     IDLRQDRQALQRLREAAEKAKIELSTMMETEINLPYITADASGPKHMQLKLSRAKFEQ
                     MTEDLLQRCRKPFEAALKDAGMDAGKLDEVVLVGGSSRMPMVQDLVRKLTNGKDPNKG
                     VNPDEVVAIGAAIQGGVLGGEVKDILLLDVTPLSLGVETMGSVMTVMIERNTTIPVRK
                     TEVYSTAADNQTAVDIHVLQGERPMANDNMSLGRFRLDGIPPAPRGIPQVEVTFDIDA
                     NGILHVTAKDKASGKEQKVTITASTNLNKNDIDRMVQEARQNEAADKKRREVIEVKNT
                     ADNLSYQIEKSLRDLGDKVPAAERDAIEAKVNQLKQAAQGDDVDQIKKLTEELQNTFH
                     ALSQQMYAQGQPQPEGGPAPSGPSGHANGDVIDGEVKE"
BASE COUNT          889 a         1104 c         1035 g         1102 t
ORIGIN      
        1 gttccaatac ccttcccgta aaaaatgaaa aaacattaat caatacaaac tcactccgaa
       61 tttcattttc gcgagtgagt ttgtcattaa gccctgattt ttcgtctttc ttttcaaaaa
      121 tcggcggttt ggctgaatcg tgcatggagt ctgctctcat aaaaccctaa tacgttggca
      181 gtaactatat cggttgcaca gcgaccaata ggataagacc aacgatttga gtataaaagg
      241 agatttctca tgcgaaaaag tactttgttc atttcagcca tgttgaccgt attcctcatg
      301 gctaccttat ttggggtgat ttcagcctat cagaacatca tggcaaccaa tcaggctgta
      361 gtcgtaacgc ctgctgagtt atcggttcag gaaccggctg ccacagccgt gccgacgatc
      421 gctgcaccca tggtgatctc accggagcaa gccaccgcca ttgccgtgga attgctcgga
      481 gattccaacg tgtatagtgt tgaagtggtg gattatgaag gcgtggccgc ctatctggtc
      541 accttcagcg cgggtgacct tgtgtatgtc agctccaccg gagaagtcct tgctgtgacc
      601 aaattggaac ctgttgttat ggtggtcccg gccggtaata ggaacgacag cggtgaaaag
      661 cccaaccgtg accagcaaca acaggaagat gatggtgacg atgacgacca tggtgaagat
      721 gaacacgaag atggcgatga ttaatcaggt acatgcacat gaatccaaaa cccgcccctc
      781 gtaaatggac cgacatacaa ctggcgatcg ccgcagtctc aatgacgtct gtgcttgctt
      841 tttggaatat gtttgccggg ccagatcgct caaaatctga agaaaaagct gctgaggcgc
      901 aacaggcttc tcaagctgca cccacctctg cggttatgtc cgtttctccc gcccctacca
      961 tgcccccggc tggatatacc attttatttg gcggcacagc cccccagcct caggtcatcg
     1021 tcatgcaatc ggggcagggc ggtaatcagg gcggcggcga tccggtcact gtcaccacat
     1081 catcataagc atgttgcacc gattttcctt tcgcgcaatg ggttcagata tcctggtcat
     1141 tttggaacag gattctgaag ccaagcccgc aattctaaaa gacattcctg catggtttga
     1201 aaactgggaa cagaccctca gccgcttcag accgacctcg gaactgtcac acctcaaccg
     1261 tacatttgac cagccgataa aggtcagtga tgcattttgg gatgtgtttc aaactgcatt
     1321 atgggccgat gagatcagcg gcgggttggt caccccaacc gttctggatt caatggtcga
     1381 agctggctac gatcgtacgt ttgacaaatt accccgcagt caattcgata tgatcgcatc
     1441 acccgccgag gcagatcatc cactctccac ggtaatttca gattgtgacg ccaaaaccat
     1501 taaccttcca aaagggactg gtctcgattt tggcggcgtg gccaaaggct gggcggcaca
     1561 tcaggcagtg gaacgcctgc aagaattcgg gccatgtctt gtaaatgcag gcggtgatat
     1621 cgccgttagc ggtccacgca tggatgggag tccctggcct gtcggggtgt cgaacccgtt
     1681 taacgtcgga gcaaacataa acgtcctgca agtgaaacaa ggcggcatcg ccacctctgg
     1741 aaaagaccgc cgcaactgga atcgcaatgg cattttttat caccatatca tcaatccact
     1801 caccggcctg cccgctgaaa ctgacttgct gcgggtaacc gttgttgccc ccacaaccat
     1861 gcaagccgaa gccgccgcaa agacagcctt cattcttggc caggaaaagg gactgcattg
     1921 gattgaggca caacccgagt ttgccgggct gctggttctt gagaccggcg acgttattca
     1981 tagccgccgt atgcaaaagt atttgtaagg agtctgatat ccatgaacgc acaaccccaa
     2041 agtgaattta acgagtcttc ggtcaacatc cagtcatttt tgttgtttct ttttgcaacg
     2101 gtcattggcc tgttggtggc tgtattgttg ttacccatct tcctgccaaa catggctttt
     2161 tccttgagcg gagacagccc gaaagcttat tggtatcttt cgcgcgccac agcctttgtc
     2221 tctctttccc tgttatggct ttccatggcg ctggggttgg gcatcaccaa taaaatggcg
     2281 cgcttatggc cgggcatgcc cgcaaccttt gccatccatg aatatgtaag cctgctgggg
     2341 ttggcctttg cgatcttcca tgctcttgtg attttaggag atcactatat caacttcacc
     2401 ttgctgcaac tgtttatccc tttcagcact gtggagtatc gcccattttg ggtggggggt
     2461 gggcagatcg ggttttacat ctgggccatt gttgcattga gtttttacat ccgccctgtc
     2521 atcgggcaaa aattctggcg ttttttgcac tatgccagct ttggcatgta tggaatgggg
     2581 atcctgcatg gcatcttcag cggcaccgac accagcctgg cgtgggcaca aaactattat
     2641 tggtattcag caggaagcct catttttctc ttctttgtcc gtattgttgg aagtttgatg
     2701 gatgtcctct tccccgccaa aaagcagact cccgctgcgc gtccgactca ggggtaactg
     2761 cttctgtttt ctgaaacatt cgctacgaaa taaaaagagc ggccaattca attggccgct
     2821 cttttgcatt gttcccttta ttcttttact tcaccgtcga tcacatcccc gttggcgtga
     2881 ccgctcggtc cactgggggc ggggccgcct tcaggctgcg gttgaccctg tgcatacatt
     2941 tgctgactga gtgcatggaa ggtgttttgc aattcttccg tgagcttctt gatttggtcc
     3001 acatcatcac cttgtgccgc ctgcttcaac tggttgacct tggcttcaat ggcatcacgt
     3061 tctgccgcgg gaaccttgtc gcccagatcg cgcagtgatt tttcgatttg ataggacagg
     3121 ttgtcggccg tgttctttac ttcaatcact tcacggcgtt ttttgtccgc agcttcattc
     3181 tggcgtgctt cctgcaccat gcggtcaatg tcattcttgt tcaggtttgt cgaggcagtg
     3241 atggtcacct tctgttcctt gcctgaagcc ttatccttcg cggtgacgtg cagaataccg
     3301 ttggcatcaa tgtcgaaggt cacttccacc tgcgggatac cgcgcggcgc cggtgggatt
     3361 ccatcgagac ggaagcggcc aaggctcata ttgtcgtttg ccattgggcg ttcaccctgc
     3421 aacacatgga tatccacagc ggtttggtta tctgccgccg ttgaatacac ttccgttttg
     3481 cggaccggga tggtggtgtt gcgttcgatc attacggtca ttacacttcc catcgtttcc
     3541 acaccgaggg aaagcggggt cacgtcaagc aggaggatat ccttcacttc gccgccgagc
     3601 acaccgccct gaatggctgc gccgatcgca accacttcat cgggattcac acccttgttg
     3661 ggatccttgc cgttggttaa cttgcggacc aggtcttgca ccatgggcat gcggctcgaa
     3721 ccgccaacca gaacaacttc atccagtttg cctgcatcca tgcctgcatc tttcaacgcg
     3781 gcttcaaagg gcttgcggca gcgctgcaac aggtcttcgg tcatctgctc gaactttgcg
     3841 cggctgagtt tcagctgcat gtgcttgggc ccgcttgcat cggcagtgat gtagggaagg
     3901 ttaatttccg tctccatcat ggtcgagagt tcgatctttg ctttttctgc ggcttcacgc
     3961 aaacgctgta aggcctgacg gtcctggcgc aggtcaatgc cctgatcttt cttgaattcg
     4021 tctgcggccc agttggtgat cttctgatcc cagtcatcgc cgccgaggtg ggtatcacca
     4081 ttggtggctt tcacttcaat gacaccttca cccacttcaa ggatggagac
//
LOCUS       DAOQJC010000222         4094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_13327_length_4094_cov_9.415945, whole genome shotgun sequence.
ACCESSION   DAOQJC010000222 DAOQJC010000000
VERSION     DAOQJC010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13327_length_4094_cov_9.415945"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PLE39_18945"
     CDS             complement(<1..723)
                     /locus_tag="PLE39_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNE70469.1"
                     /translation="MIKLDEVKMSAEENGKWGMVIDQDICTGCQACVAACAMENNISF
                     VGEEDSGYGRSMHWIRIERFWEGEYPEVKMTPQQPMMCQQCGHATCEPVCPAFATVHS
                     QAEQLNLQVYNRCVGTRYCANNCPYQVRAFNWRDYQRPEPLSNQLNPDVTVRRMGVME
                     KCTFCIQRIHKAQDKAKAEGREVVDGEFTTACAQACPANAIVFGRVDNPESLVSQLAH
                     RGHGAKHLEELGTLPNVTYFKGG"
     gene            complement(726..2903)
                     /locus_tag="PLE39_18950"
     CDS             complement(726..2903)
                     /locus_tag="PLE39_18950"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNE70470.1"
                     /translation="MSQQFSRRDFLKLAGVGAATTAILTGCGPASRYVKREPYMQMPE
                     YNYNGQSTYYATTCRECAAGCGLIVRTMQGRALKTEGNANNPLNLGKTCARGQATLHG
                     LYNPDRVTDPMKGGSTITWDEATQTVADALKNNKPEEIAFLMGMAPDHLFDLVTDLAN
                     ATGINAPVRFGALGMFEARATLSKAAENLFGEAKMPFFDVGGAEVVLSFGANYLETWL
                     SPVSYTRGFAGLREAKTKQRGVFIQLEARMSSTAAKADEWLPIRPGTEALVALAIGRI
                     ASELRGGPMPRAFSSVDPKDAAKKSGIKLETLEHIASLFTGAKVALAIPGGSALGQSN
                     GLTVAEAVLALNAVADNFGKPGGVYFSPLAPNQTSYQRPASAKEMQEFIQKMADGKFK
                     VLFVHGVNPVFELPAALGFKDALKGVGQVISFATFPDETASEAGFIFPDHHGLESWGY
                     QRVATGSNQPVLSGSQPVVSPFYNTRATADVLIAAAQLAGGAFATALPFKDEVEFLQS
                     KVAPLMSEADGFFSAADIDTFMATFQQYGGWWKLSDGRGVPGTADVLNRNMDGAEAEF
                     VGEGEFFFAPFVSPTLAEAGANKPWLQELPDPTTTVMWNTWIEVNPETAHELGLHNDD
                     VVKVISEFGELQAYVYLYPAIRPDTIAIPFGQGHTAYGRYAENRGANPANLLGVHFNE
                     AGDFAFSGTKVKVEKVSSAVSGVGLSRMESRIGVYGEALGEEH"
     gene            complement(2924..3478)
                     /locus_tag="PLE39_18955"
     CDS             complement(2924..3478)
                     /locus_tag="PLE39_18955"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNE70471.1"
                     /translation="MIKRIFDWLKQPLGLIAVGIAFLALFSVAAYGLYLTQQPPAQPI
                     QFPHQTHVAFGIQCLYCHPNAAKGPTAGIPAQAKCWACHQQIAKTQTSALLAPLKDAV
                     ISGTPIEWVPVAIVPDFVQFNHRAHIAAGKNCEECHGDMSKVTIAQNPQVMNMGWCLD
                     CHVKNAGEDQVKLVKLTDCGTCHY"
     gene            3814..4068
                     /locus_tag="PLE39_18960"
     CDS             3814..4068
                     /locus_tag="PLE39_18960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70472.1"
                     /translation="MKTIYVFGLAFAFLLLVFVFTSVNFNPSLVQAQNQANSFTSLQT
                     TPTPPVEGESVIGSTDGIMLMGVVITVIVVVPLLFHRKKH"
BASE COUNT          990 a         1085 c         1069 g          950 t
ORIGIN      
        1 ccctcccttg aagtaggtca cgttgggaag cgtgccgagt tcttcaagat gtttggcgcc
       61 atgaccacga tgagccagtt gagaaaccag cgattcgggg ttatctacgc gcccaaaaac
      121 gatcgcattc gcagggcagg cctgtgcaca cgcggtggtg aattcaccat ccacaacctc
      181 gcgtccctcc gctttggcct tgtcctgtgc tttgtgaatg cgctggatgc agaaggtgca
      241 tttttccatt acacccatgc ggcgtactgt aacatcaggg ttgagttggt tggaaagcgg
      301 ctcagggcgc tgatagtcgc gccagttgaa tgcacgcacc tggtacgggc agttgttcgc
      361 gcaataacgc gtacccacac agcggttata cacttgaagg ttcaattgct cagcctgtga
      421 gtgaacagtg gcaaatgccg ggcagaccgg ttcacaagtg gcatgcccgc attgctggca
      481 catcatgggc tgttgggggg tcatcttcac ttcaggatat tccccttccc agaagcgttc
      541 aatgcgaatc cagtgcatgg aacggccata gcctgaatct tcttcgccga caaaggagat
      601 gttattctcc atggcacagg cggcaacgca ggcctgacaa cccgtgcaaa tgtcctggtc
      661 aatgaccatg ccccatttgc cgttttcttc cgcgctcatt ttgacttcat caagtttaat
      721 cataattaat gctcctcacc cagcgcttcg ccatacacac caatgcggct ttccatgcga
      781 gacagaccaa caccgcttac agcactggag accttttcaa ctttgacctt tgtaccgcta
      841 aatgcaaaat caccggcttc gttaaagtga acgccaagca aatttgcagg attggcgccg
      901 cgattttcag cataacgccc atatgcagtg tgaccctgcc caaacggaat ggcgatcgtg
      961 tcggggcgaa ttgcagggta caggtaaaca tacgcctgta actcaccaaa ctcactgatc
     1021 accttgacga catcatcatt atgcaggccc agttcatgag cagtttcagg gtttacttca
     1081 atccaggtat tccacataac agtcgtggtt gggtcgggca attcctgaag ccagggtttg
     1141 tttgcacctg cttcggcaag ggtcggtgaa acgaatgggg cgaagaagaa ctcaccttca
     1201 ccaacaaatt cagcttcggc accatccatg ttgcggttga gaacgtctgc tgtgccgggc
     1261 acgccacggc catcagaaag tttccaccat ccaccatatt gctggaaggt agccatgaaa
     1321 gtatcaatat cggctgcgga gaagaagcca tcggcttcgc tcatcagcgg ggcaactttg
     1381 ctctgcaaga attcaacttc atctttgaac ggcaaggcag ttgcgaatgc gccaccagcc
     1441 agctgggcgg cagcgatcaa gacatcggct gtagcgcggg tgttgtagaa cggtgataca
     1501 acaggctgtg agccggagag gacgggttgg ttggaaccag tggctacacg ctggtatccc
     1561 caggattcaa gtccgtggtg atcggggaaa atgaaaccag cttcacttgc ggtttcatcg
     1621 gggaaggtcg caaaggaaat cacctgaccc acacctttta atgcatcctt gaaacctaaa
     1681 gcggcaggaa gttcgaatac agggttgaca ccatgcacga acaaaacctt gaatttcccg
     1741 tcagccatct tttggatgaa ttcctgcatt tccttggcgg aagcagggcg ctgatagctg
     1801 gtctggttcg gggccaacgg cgagaagtac acaccaccag gtttgccgaa gttatcggca
     1861 actgcattca aagcaaggac ggcttcggca accgtaaggc cgttgctctg accaagagcg
     1921 gaaccaccgg ggatggcaag cgccaccttc gcgccagtga ataggctggc aatatgctca
     1981 agggtttcga gtttgatgcc cgatttcttg gcagcatctt tcggatccac acttgagaaa
     2041 gcgcggggca tggggccgcc acgcaattca gaggcgattc tgccgatcgc aagcgcaacc
     2101 agtgcctcgg tcccggggcg aatcgggagc cattcatccg ccttggcggc ggtggaagac
     2161 atacgcgctt cgagttggat gaagactccg cgctgcttgg ttttcgcttc acgcaggcca
     2221 gcaaagccgc gagtatatga aacaggagaa agccatgttt cgaggtagtt tgcaccaaag
     2281 gagaggacca cttccgcccc acccacatca aagaagggca tcttggcttc accgaaaaga
     2341 ttctcagcgg ccttgctcaa agtggcacgg gcttcgaaca tacccaaagc accaaagcgg
     2401 accggggcat tgatgcctgt tgcattggcg aggtcagtta ccaggtcaaa gagatgatcg
     2461 ggggccattc ccatcaggaa ggcgatctct tcgggcttgt tgttcttgag agcatctgca
     2521 accgtctgag ttgcttcatc ccatgtgatc gtgctgccgc ccttcattgg gtcggttacg
     2581 cgatcggggt tgtacaagcc gtgcaatgtg gcctgtccgc gtgcgcaagt ctttccgagg
     2641 ttcagcggat tgttcgcatt gccttcggtt ttgagggcac gtccctgcat ggtgcggaca
     2701 atgagtccgc atccggccgc acattcgcgg caagtggtgg cgtaataggt gctctgaccg
     2761 ttatagttgt attcgggcat ctgcatgtac ggttcgcgtt tgacataccg tgaagcaggc
     2821 ccacaaccgg tgaggatcgc agtcgttgcc gcgcccacac cagccagttt cagaaaatca
     2881 cgccgggaaa attgttggct catattgatt cctttcgccc ggtttaatag tgacaagtgc
     2941 cgcagtcggt gagtttgacc agtttgacct gatcttcacc ggcattcttc acgtgacaat
     3001 ccagacacca ccccatgttc atcacctgcg ggttttgggc aatggtcact ttgctcatgt
     3061 cgccgtgaca ttcttcgcag ttcttcccag ccgcaatatg cgcacggtga ttaaactgaa
     3121 caaagtctgg cacgatcgca accggcaccc actcgatggg ggtaccactg atgactgcgt
     3181 cctttaacgg tgcaagcagg gcgctcgtct gtgtcttggc gatctgttgg tggcaggccc
     3241 aacatttggc ctgggctggg atgccagcgg ttggtccctt cgccgcattt ggatggcaat
     3301 acaaacattg aataccaaat gcaacgtgag tctgatgggg gaactgaatg ggttgtgcag
     3361 gtggctgctg ggtaaggtac aaaccataag ccgctacact gaacaacgcc aggaaagcga
     3421 tccccaccgc gatcagacca agaggctgtt tcagccagtc aaaaattctt ttgatcatgg
     3481 atgctcctga aatgaatctt ttaagcgcaa cgccgccggg tgaaggcggc gaaggctact
     3541 ttacatggat acaaacattg tacaaatgca aaagttacga gccgaaaccc gccgccccat
     3601 taaatgcata tacataaaac ccgtcccgat attcatcagg acgagaaatg ggcgtagaat
     3661 tttcgtcaga ttactcatgc attctccaaa tacaaatttg ctgaaccccg ccagcaaatt
     3721 aactcggcgg attatacatc gaaagtgcag aaaagtacat ataagagtta aattagagac
     3781 ttttgagccg tgttacaatt ggcaacgata acaatgaaaa cgatctatgt ttttgggctg
     3841 gcctttgctt ttcttttgct tgtttttgta ttcacgagcg taaacttcaa cccatccctt
     3901 gtgcaggcgc aaaatcaggc aaattccttc acgtcactgc aaacaacccc caccccacca
     3961 gttgaaggcg aatcggtcat cggttccaca gacggcatca tgctgatggg agtggtcatc
     4021 acggtcattg tcgttgtacc gttgctcttc catcgaaaaa aacactaaac ctacaaaaca
     4081 aacatcccgc tgct
//
LOCUS       DAOQJC010000223         4049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_13536_length_4049_cov_7.931898, whole genome shotgun sequence.
ACCESSION   DAOQJC010000223 DAOQJC010000000
VERSION     DAOQJC010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13536_length_4049_cov_7.931898"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..345
                     /locus_tag="PLE39_18965"
     CDS             58..345
                     /locus_tag="PLE39_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70473.1"
                     /translation="MTLPTILFALVVALLLGAMYHFFRGGGGWRLMLFFGLSILGFAL
                     GQLLAMWQGWRILMFGSLDLGMGGVGSLLMLVTGEWLSRIDVKEIKKESGV"
     gene            420..1055
                     /locus_tag="PLE39_18970"
     CDS             420..1055
                     /locus_tag="PLE39_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNE70474.1"
                     /translation="MEFLKSAIDLFLHLDEYLAKIISDYGTWTYGILFAVIFVETGLV
                     VMPFLPGDSLLFAAGTFAALGSLNVWFIVGLLSVAAVLGDAVNYSIGHYLGERAYNIK
                     WIKREYLDKTHAFFEKHGGKAIFLARFVPIVRTFAPFVAGIGKMSYGYFATYNIVGGI
                     SWVLIFTLMGFFFGNIPFVKANFELVIIAIILVSVVPMIYEWWKHRQEAKK"
     gene            1109..1471
                     /locus_tag="PLE39_18975"
     CDS             1109..1471
                     /locus_tag="PLE39_18975"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNE70475.1"
                     /translation="MKYAAVLFTIFIILVIILADRGLLGPLHFIYDFPYGDKAGHFIL
                     YGLLAFFITLASIRSFLRLDPKRVALSIGLILALLIGAEEYSQKFFANRTFDLIDLLA
                     SYIGLIVGGWAALKQARK"
     gene            complement(1540..>2562)
                     /locus_tag="PLE39_18980"
     CDS             complement(1540..>2562)
                     /locus_tag="PLE39_18980"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNE70476.1"
                     /translation="KGDGTLPVPGWTDEYEWQGFLPFEELPYTLNPEEGYIVTANNQV
                     HPRDYPYFISADWDYGFRANRIVEMITTAPGKIDIAYIQQMHGDSFDANGPVFVPLLL
                     NEEFASDLNPALDTLKNWDYQGRADSTSAAVFNAFWRALLQNTFNDDMPEERYYPDGG
                     SRWNEVMRHMAENPNDPFWDDKTTPDVTETMQDMIQKSFPQGIAELQEIFGKDMSKWK
                     WGDMHASTFRNGTLGESGVSLIEDLFNRGPFPTSGGEAIVNATGWSVKDGYETNWLPS
                     MRMIVDLGDLNNSVTVHTTGESGHAYHPHYADMAPMWAGIQYYPMWWNQDSVIGDAEG
                     HLILKP"
     assembly_gap    2563..2662
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2663..3982)
                     /locus_tag="PLE39_18985"
     CDS             complement(<2663..3982)
                     /locus_tag="PLE39_18985"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNE70477.1"
                     /translation="MKKLGRFVGRMLIGVVVLALIAGAGGMYYFKSYLPNTVAPKSFP
                     QIDGEISVSGLNGQVDIYRDAMGIPHIYADTTHDLFFAQGYVHAQERFWQMDAWRHIG
                     SGRLSEMFGSGQADTDAFLRTLGWKQTAEEEWKQLDPESKAILEAYVEGVNAYLKDHS
                     DTALSLEYAILGLLSPDYEIEEWTPVNSLTWGKAMAWDLRGNMGEEIERAVLLKTLTP
                     EQVAQLFPAYPEDHPVIVNNIGEGGSAASAGSEATLDYSQVPFENISENVALLDSFLG
                     PWNDGIGSNSWAVSGKLSDTGMPLLANDPHLGIQMPSIWFQVGLHCNEKTAECPFEVA
                     GFSFAGVPGVIIGHNDQIAWGFTNVGPDVMDLYIERVNPENPNQYDVNGTWVDFDTRE
                     EVIKMSDGTTQTITVRSTRHGPVISDQYGPLKDVGDPEDTEFVPFKDR"
BASE COUNT          839 a          964 c         1070 g         1076 t
ORIGIN      
        1 aatttttgcc cttgatcgta accttcaacc ttcaaccttc aaccttcaac cttcatcatg
       61 acccttccaa ccattctctt tgcccttgtt gttgccctcc tccttggggc gatgtatcat
      121 ttttttcgtg gcgggggcgg ctggcgtttg atgttgttct tcggtttgag tattctcggt
      181 tttgcgttgg ggcagctgct tgccatgtgg cagggctgga gaattttgat gtttggatcg
      241 ctcgatctgg gaatgggcgg ggtgggcagt ttgttgatgc tggtgactgg ggagtggctg
      301 agccgaatcg atgtgaagga aatcaaaaaa gaaagcgggg tataatcccc gcccgttaaa
      361 aacggagcct tgggttttca ggcctggcct ccgatatcta ttgaaagaga gaataaaaaa
      421 tggaatttct gaaaagcgct attgacctct tcctgcattt ggatgagtac cttgccaaga
      481 tcatttcgga ttacggcaca tggacatacg gcattttgtt cgctgtgatc tttgtggaaa
      541 cagggttggt ggtcatgccc tttctgcctg gcgattcact gctctttgca gcaggaacgt
      601 ttgctgcact gggttcatta aatgtgtggt tcattgtggg tttgctttcg gtggcggctg
      661 tgctgggtga tgcggtgaat tactccatcg gtcattatct gggcgagcgc gcctacaaca
      721 tcaagtggat caagcgcgaa tatctcgata agacccacgc ctttttcgaa aaacatggtg
      781 gcaaggccat ctttcttgcg cgcttcgttc ccattgtgcg cacctttgcg ccgtttgtgg
      841 cagggatcgg taaaatgtcc tatggctatt tcgccaccta caacattgtg ggcggaattt
      901 cctgggtttt gatctttacc ctgatgggtt tcttcttcgg aaacattccc tttgtgaagg
      961 cgaatttcga attggtcatc attgccatta tccttgtctc ggtggtgccg atgatctacg
     1021 agtggtggaa gcatcgtcag gaagcgaaga agtaatttcg aaatcgcgcg ccgtgccatt
     1081 tgtgtggcgc ggcgcgtttt ttatctccat gaaatacgct gctgttcttt ttacgatttt
     1141 tattattttg gtcatcattc ttgcagaccg tggattgctg ggtccgctcc actttattta
     1201 cgattttccc tacggtgaca aggcgggaca cttcatcctg tacgggcttc tcgccttttt
     1261 catcacactt gcctccatcc gctcgtttct tcgcctggac ccgaaacggg tggcgctttc
     1321 aattggcttg attctcgccc tgctcatcgg cgcagaggaa tattcacaaa agttcttcgc
     1381 caatcgcacc tttgacctga ttgacctgtt ggcgagttat attggtttga tcgttggggg
     1441 atgggcagcc ttgaaacagg cacgcaaata aaaaatcttt cctctttctt cgtttattga
     1501 agaaagagga aagatataag aaattcagag tgttttttac tatggtttca agatcaagtg
     1561 tccttcggca tcgccgatga cagagtcttg attccaccac atggggtaat actgaatgcc
     1621 cgcccacatg ggagccatat cggcatagtg cggatgatag gcatggcctg attcgcctgt
     1681 ggtgtgaacg gtgacggaat tgttcaaatc acccaggtcc acgatcatgc gcatggaggg
     1741 cagccagttg gtttcgtagc catccttcac cgaccagccc gtggcgttga cgatggcttc
     1801 gccgccgctc gtggggaagg gtccacggtt gaagaggtct tcgatcaacg agacgcccga
     1861 ttcgccgagg gtgccattgc ggaaggtgga ggcgtgcatg tcaccccatt tccatttgga
     1921 catgtccttg ccgaagattt cctgcaattc ggcaatgccc tgcgggaagg atttttggat
     1981 catatcctgc atggtttcag tgacatcagg ggtggttttg tcgtcccaga acgggtcgtt
     2041 ggggttttcg gccatgtgac gcatgacctc gttccagcgt gagccgccat cgggataata
     2101 gcgttcttca ggcatgtcgt cgttgaaggt gttttgcagt aaagcgcgcc agaatgcatt
     2161 gaacacggcc gcggatgtgg aatctgctcg gccttggtaa tcccaattct ttagcgtatc
     2221 cagcgcgggg ttaaggtcag atgcgaattc ttcattcaaa agaagcggca caaagacagg
     2281 tccgtttgca tcgaaggagt cgccgtgcat ttgttgaatg taggcgatat cgatcttgcc
     2341 cggggcggtg gtgatcatct cgacaatgcg gttggcacgg aagccgtagt cccaatcggc
     2401 agagatgaaa tagggataat cacgcggatg cacctgattg ttggcggtga cgatgtagcc
     2461 ttcttcggga ttgagggtgt aaggcagttc ttcgaaagga aggaaaccct gccattcgta
     2521 ttcatccgtc caaccgggga cgggcagtgt gccatcgcct ttnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnacggtctt tgaatgggac gaactcagtg tcttcggggt
     2701 cgcccacatc tttgagcgga ccatattggt cggagatgac gggcccatgg cgggtgctgc
     2761 gcacggtgat ggtctgcgtt gtgccatcag acattttgat gacttcttcg cgggtatcaa
     2821 aatccaccca ggtgccgttc acatcgtatt gattcgggtt ttcagggttg actctttcga
     2881 tgtacaagtc catcacatca ggcccgacat tggtgaagcc ccaggcgatc tggtcattgt
     2941 ggccgatgat cacaccgggt accccagcaa atgaaaagcc cgccacttcg aagggacatt
     3001 cagcggtttt ctcattgcag tgcaagccga cctgaaacca gatcgaaggc atttgaatgc
     3061 caaggtgtgg atcgtttgcc agcagcggca ttcctgtatc agagagtttg cctgaaaccg
     3121 cccacgaatt tgaaccgatg ccatcattcc acgggccaag gaaagaatcg agcaaagcca
     3181 cattttcaga aatgttttca aacggcactt gtgaataatc aagggtcgct tcgctgcctg
     3241 cacttgcagc cgaaccgcct tcgccaatgt tgttgacaat gacaggatgg tcttcaggat
     3301 aggcagggaa aagctgtgcc acctgctcag gggtgagcgt tttgagcaga acggcacgtt
     3361 cgatctcttc gcccatgttg ccgcgcaggt cccaagccat ggccttgccc catgtcaggc
     3421 tgttgacggg agtccattct tcgatttcgt aatccgggct gagcagcccc aggatggcat
     3481 attcaaggct gagggctgta tcggagtgat ccttcaaata agcgttcaca ccttctacat
     3541 aggcctccaa aatggccttt gattcagggt cgagttgctt ccactcttct tcggcggttt
     3601 gcttccagcc cagcgtgcgc agaaatgcat ctgtgtcggc ttgtccgctg ccgaacattt
     3661 ccgaaaggcg gcccgaacca atgtgccgcc atgcatccat ctgccagaag cgttcctgcg
     3721 cgtggacgta tccctgcgcg aaaaatagat cgtgggttgt atcagcatag atgtgcggga
     3781 tccccatcgc gtcgcggtag atatccacct gaccgttgag tccgcttacg ctgatctcac
     3841 cgtcaatttg cggaaaggat tttggcgcga cggtattcgg taaataagac ttgaaataat
     3901 acattccccc ggcgcctgcg atcaacgcga gaacgacaac gccgatcaac attcgcccaa
     3961 cgaacctgcc taactttttc atgcttcctc cagttggaaa attttgtgga ttatagtcac
     4021 ctgtgggtgg attgcatagc acataaaat
//
LOCUS       DAOQJC010000224         3907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_14274_length_3907_cov_8.015576, whole genome shotgun sequence.
ACCESSION   DAOQJC010000224 DAOQJC010000000
VERSION     DAOQJC010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14274_length_3907_cov_8.015576"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PLE39_18990"
     CDS             complement(<1..919)
                     /locus_tag="PLE39_18990"
                     /inference="COORDINATES: protein motif:HMM:NF024926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance efflux transporter family
                     protein"
                     /protein_id="HNE70478.1"
                     /translation="MTRLIAIGSLAALFFSSTFVLNRAMSLQGGHWAWTSSLRFGFML
                     IFLILLLLATQGKKALAEVRRVFLSHWLFWILAGSIGFGVFYSLITFSSQYAAGWVVA
                     TTWQTTILATPIVLVFFGRKVPTKGLLFTGIIFVGIILVNIEHAALTSLREVLFSALP
                     VLIAAFAYPIGNQLVWEARMGHNARIPKIENPILENGFARVLLLTLGSIPFWVLILLF
                     SSPPAPASGQLLSTALVALLSGVIATTLFLYARHLCHQPYEIAAVDATQSMEVVFSLI
                     GEIVFLHGSFPGPIGFVGVALTILGLIAYM"
     gene            complement(920..1732)
                     /locus_tag="PLE39_18995"
     CDS             complement(920..1732)
                     /locus_tag="PLE39_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70479.1"
                     /translation="MLSNLSRWLTYTCALLYAILGLFLFLFPAQLAPVFAWKVTAFMT
                     ITIGGWCLGNAWLAYINARRWEWKLVYSSLVYLWLFGIGELIVLFVFRDKLKLEHPIA
                     WLYFITLIVNALAAFVGVFDYLRIRPSNQSNGPTFTKIQSLPAFAFVLFVGFLGLYGL
                     TAQIGDIGTNAGIFPEVMSLFTLRSFGVFYFALALGVVPYFWDRNLNAILHHSFAAYP
                     LVVFITTAAFIYIRLFDFAKRPGGLAYFGVYLAVGIPLIFVFIKYGTGGSRN"
     gene            complement(1912..3294)
                     /locus_tag="PLE39_19000"
     CDS             complement(1912..3294)
                     /locus_tag="PLE39_19000"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNE70480.1"
                     /translation="MTPKFYPPEPYRIKVTEPIRLISPEAREIALKEAGYNLFSMKAE
                     DIFIDLLTDSGTGAMSQEQWAAIMRGDESYAGARSYQRLKTAVEDIFGFKFFVPTHQG
                     RAAENILSACLVKPGNYIPSNMHFDTTDANIRARGGRPTNLVIDEAFDPANPHPFKGN
                     MDINKLRAFIQKVGVANIPFGMVTVTNNAGGGQPVSMENLKAVAAVYREFNIPFFIDS
                     ARYAENAYFIKLREKGYENKSVIDIAREMYALADGMTMSAKKDAIVNIGGLLCLNDET
                     LFGAIRNELILREGFPTYGGLAGRDLDAMAVGLYEGLDEAYLASRLTQTAYLAARLNE
                     AGIPTIQPAGGHAVYLDAAHVFPNIPQSEFPGQALAVELYREGAIRGVEIGSVMFAYP
                     DPDSGKMIHPKLELLRLALPRRTYTQSHMDYVADCAAQIKSRADKVRGYKFTFAPELL
                     RHFTARFEPL"
     gene            complement(3549..>3907)
                     /locus_tag="PLE39_19005"
     CDS             complement(3549..>3907)
                     /locus_tag="PLE39_19005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70481.1"
                     /translation="REQWAEAESLAREALALAEKVGRQELIAGDCHLLAKALFKQNPV
                     DAVGATRPVQKSALSGNVEAQENVLSDREGSPLHEALLLSRRAVEIYTRLRHPDLQSA
                     QETLAEIERALQKSER"
BASE COUNT          958 a          982 c         1103 g          864 t
ORIGIN      
        1 tcatgtaggc gatcaaccca aggatggtga gtgcaactcc cacaaaaccg atcggcccgg
       61 ggaatgaacc atgtaaaaaa acgatctcgc cgatcaagga aaataccact tccatggatt
      121 gggttgcgtc cacggcggcg atctcgtagg gttgatggca gagatggcgg gcgtaaagaa
      181 aaagggtcgt ggcaatgacg cccgacaaca gagcgacaag cgccgtgctg agcaattgtc
      241 cgctggcggg ggcgggggga gaactaaaca ggagaatcaa cacccaaaac gggatcgagc
      301 ccaaggtcaa gagaaggact cgggcgaagc cattttccag aatcggattt tcgattttgg
      361 gaatccttgc gttgtgaccc atgcgcgcct cccaaaccag ttgattgcca atggggtagg
      421 caaaggcggc gatcaaaaca ggcagggcgc tgaacagcac ttcacgcaaa ctggtcaacg
      481 cggcatgttc aatgttgacg aggatgatcc ccacaaaaat gattcccgtg aaaagcaaac
      541 ctttggtggg caccttgcgc ccgaaaaaga ccaacacgat gggggtggca aggatggtgg
      601 tctgccatgt ggtggcaacg acccagcccg cggcatattg tgaactgaac gtgatgagtg
      661 agtaaaaaac accgaagcca atgctgcctg caagaatcca gaacagccaa tgactcagaa
      721 agacgcgcct tacttcagcc agcgcttttt tgccctgtgt ggcgagcagc aataagatca
      781 agaagatcag catgaacccg aagcgcagac tggatgtcca cgcccagtga ccgccctgta
      841 agctcatggc gcggttgagg acgaaggtgc tgctgaagaa taaagcggca aggctgccga
      901 ttgcgatgag tcgtgtcatt taatttctac tgcctcctgt gccatatttg ataaagacga
      961 agatcaacgg aattcccacc gcaagataca caccgaaata cgccagtccg cctgggcgct
     1021 tggcaaaatc gaagaggcgg atgtatataa atgcagcggt agtgataaag acaaccagcg
     1081 gatacgccgc gaaggaatgg tgcaggatgg cgttcaggtt tctgtcccaa aagtagggca
     1141 caactcccag tgcaagcgca aaatagaaaa ccccgaaact ccgcaaggtg aacaatgaca
     1201 tgacttcagg gaagatgccc gcattggtgc cgatatcgcc gatctgtgcg gttaatccat
     1261 acaatccgag gaagccaacg aacagtacaa atgcaaatgc gggcaggctc tgaattttcg
     1321 tgaaagttgg tccgttggat tggttggacg gacgaatacg aagataatca aaaacaccga
     1381 cgaatgccgc aagagcattg acgatcagcg tgatgaaata caaccaggca atcggatgtt
     1441 caagtttcaa cttgtcgcgg aagacgaaca gcacgatcaa ctcaccgatg ccgaatagcc
     1501 atagatagac cagcgaggag taaaccagtt tccactccca gcggcgggcg ttgatataag
     1561 ccagccatgc attgccgaga caccagccgc cgatggtgat ggtcatgaag gcggttactt
     1621 tccacgcgaa gacaggcgca agttgtgctg ggaataaaaa taaaaacagg cccaatattg
     1681 catacagtaa agcacaggtg taagtcagcc agcgggaaag gttcgatagc atggtttctc
     1741 caattttctg tagataaagc tgtgccctat tgtagcagtt acttctcttt atctttcgta
     1801 aaataaaaag tcaccagctg attggactgc tgatgacttt ttatttaatc tttatcattg
     1861 cgaggagggt gctttcccaa cgaacaaggt ctctccttgc gctgacgtat gctaaagcgg
     1921 ttcaaaccgt gcggtgaaat gcctgagcag ttcaggtgcg aaggtgaatt tgtagccgcg
     1981 cactttgtcg gcgcgggatt tgatttgagc ggcgcaatct gccacatagt ccatgtggct
     2041 ttgtgtgtac gtgcggcgcg gaagggcaag ccgcagcaat tccagcttgg ggtggatcat
     2101 tttgccagaa tcaggatcgg ggtaggcgaa catcactgag ccgatctcca cgccgcggat
     2161 cgcgccttcg cggtacaact ccacggccag cgcctgcccg gggaattcgc tctgcggaat
     2221 attcgggaag acgtgcgccg catcgagata cacagcatgc ccgcctgcgg gttgaatggt
     2281 ggggatgcca gcctcgttga gccgcgccgc aagatacgcg gtttgcgtaa gccgtgaggc
     2341 gagataggct tcatccagcc cttcgtacaa accgaccgcc atcgcgtcga ggtcacggcc
     2401 tgccagcccg ccgtaagtgg ggaaaccttc ccgcaggatc aattcgtttc tgatcgcgcc
     2461 gaacaaggtt tcatcgttca ggcacaacag cccgccgatg ttgacgatgg catccttttt
     2521 cgcggacatg gtcatgccgt cggccagcgc gtacatttca cgcgcaatgt cgatcaccga
     2581 tttgttttcg tatccttttt cgcgcagttt gataaagtag gcgttctcgg catagcgcgc
     2641 ggaatcaatg aagaagggga tgttgaactc gcgatagaca gccgccaccg ctttgagatt
     2701 ttccatcgaa acaggctgac cgcctcctgc gttgtttgta acggtcacca ttccgaaggg
     2761 gatattcgcc accccgacct tttgaatgaa ggcgcgcagt ttgttgatat ccatgttgcc
     2821 cttgaaggga tgcggattcg cagggtcgaa ggcttcgtca atgacgaggt tggtggggcg
     2881 cccgccgcgc gcgcggatgt tggcatcggt ggtgtcgaaa tgcatgttcg atgggatgta
     2941 attgcctggt ttcaccagac aggccgagag gatgttttcc gcggcacgtc cctgatgggt
     3001 ggggacaaaa aacttgaaac cgaaaatatc ttccacggcg gttttcaagc gttggtacga
     3061 gcgcgcaccc gcataggact catctccgcg catgatcgcc gcccattgtt cctgactcat
     3121 ggcgcctgtg ccagagtcgg tcagcaggtc aatgaagatg tcctctgctt tcattgagaa
     3181 taggttataa cctgcctctt tcagcgcgat ctcccgcgct tcaggcgaga tcaggcggat
     3241 gggttccgtg accttgatgc ggtacggttc gggggggtaa aacttggggg tcatttgggt
     3301 ctcctgaaat ttgaggtttt gtcaaatttt gggtagcgag cctggcatgt gttcccctga
     3361 tggccattcg gtctccaatg acgggcgtat tttacaagta atctacattt tgaggttgta
     3421 acaaatgtca tgaattcccc atatttgttt gatgtcattg cgagggcggt gcatttcgcc
     3481 cgaagcaatc caccattaat gaggagattg cttcgctcgc ccaaagggct cgctcgcaat
     3541 gacatatttt atctttctga cttttgcaat gctctttcga tctccgccag cgtttcctgc
     3601 gcggactgca aatcgggatg acgcaggcgc gtgtaaatct caacggcgcg acgggataat
     3661 aataatgcct cgtgtagggg cgacccttca cggtcagaca agacgttttc ttgcgcttct
     3721 acattgccag acaaggcact tttttgcaca gggcgggtcg cccctacagc atcgacgggg
     3781 ttttgtttga acagggcttt ggcgaggagg tgacaatcgc ccgcaatcaa ctcctgccgc
     3841 ccgacttttt ctgccagcgc gagggcttcg cgggcgaggg actcggcttc tgcccactgc
     3901 tcgcggt
//
LOCUS       DAOQJC010000225         3807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_14894_length_3807_cov_10.506397, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000225 DAOQJC010000000
VERSION     DAOQJC010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14894_length_3807_cov_10.506397"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLE39_19010"
     CDS             complement(<1..209)
                     /locus_tag="PLE39_19010"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HNE70482.1"
                     /translation="MSITTTPEISIEALRSGSRAEFARMVDVHSPLIYRLGLKMLGNP
                     QDAEDVLQNTFLNALTHLPDFEGRS"
     gene            326..769
                     /locus_tag="PLE39_19015"
     CDS             326..769
                     /locus_tag="PLE39_19015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70483.1"
                     /translation="MDKLLNEQVVGQIKQAFAAITNPVQILFFGSKDNCEYCGETGQL
                     VKEISEIDDRVHLSTHDLHEDAELAAQFRVDKAPVIVIAGRDGDTVVDLGIQFSGIPS
                     GYEFSTLINDILFASKRDSGLGAATREFLKSLTKPLHLQVFVTPT"
     gene            785..994
                     /locus_tag="PLE39_19020"
                     /pseudo
     CDS             785..994
                     /locus_tag="PLE39_19020"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
     gene            1011..1610
                     /locus_tag="PLE39_19025"
     CDS             1011..1610
                     /locus_tag="PLE39_19025"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNE70484.1"
                     /translation="MSNANKQKYPGKRQKRRNSSTAMGIGLILFGVAFFLWLLGSSGK
                     ASLPTADEISIVPAEVNFPAPQLALENLNGEKQTLEDYQGRIVLVNNWATWCPPCKAE
                     MPILKQYHDTHAAEGFTVIAVEAGDAKDTVAEFAKSLGLPFPVWLDPESASINAIHNG
                     SLPNSYVIDRTGTVRYAWTGEVTMTMLEKYITPLIAENN"
     gene            complement(1676..>3807)
                     /gene="alaS"
                     /locus_tag="PLE39_19030"
     CDS             complement(1676..>3807)
                     /gene="alaS"
                     /locus_tag="PLE39_19030"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNE70485.1"
                     /translation="RGTDHVCGVNGECTRYLELWNNVFIQYNLFDDGRLELLPAKHVD
                     TGMGFERIVSVLQGVDSNYKTDLFTGSLEVLRALTGHTEKEMLANFTPYRVICDHVRS
                     ASFLIADGVVPGNAGRNYVARMIVRRAARFGTKIGLTEPFLAKVAEAVIEQYGDFYPE
                     LRKTQPAILDNLTREEIRFARTVEAGTAHLENLLSELRSTNQTVLDGHKAFDLYATYG
                     LPFEISRDIAREQGLDVDEKGFTTAKEEHAIASGGGKAMGKLGGEDAEFFAGILKDLQ
                     AKGKLSKDGVEYDPYNGLDVSRYSNLEILALVVNGQSVDSANLDDQVEVILPKTGFYI
                     EAGGQVNDTGFIRGEGWEIEVTGIRRASAGVIVHIGEVISGHPKVGDKATAEVDANRR
                     HDIMRNHTATHLLHKALHEVLSDQAKQAGSLVSPTHLRFDFNHPEAMTPDQVERVERM
                     VNEAVAMDMPVVKVTKSLDEAKKEGAMALFGEKYGETVRTISIMEGGEDKYSYELCGG
                     THLERTSDVGAFIIVSESSAAAGVRRIEAVTGRGAYDLIHKRFKTLKQAAGSLKAAVE
                     EVPAKVDALQDEVSELKKELANLRTQSALSAFNLQLSNVQTVKDVSVLAAEIVNADTD
                     TLRSLADKFREKYPKAGAAVLVTGTTVIAVVTEDLVKRGLKAGDLITGIGGKGGGRPN
                     LAQGSLPDGNVNEALGKLAKVVEEKVK"
BASE COUNT          908 a         1036 c          991 g          872 t
ORIGIN      
        1 cttgaacgac cttcaaaatc agggagatgt gtcagcgcat tcaaaaatgt gttctgcaaa
       61 acatcttccg cgtcctgggg gttcccaagc attttcagcc ccagcctgta aatgaggggg
      121 gaatgcacgt caaccatgcg cgcaaattct gcgcggctgc ctgaacgcag ggcttcaatg
      181 gaaatttcag gtgtggtcgt aatactcatt ggatgatatt cgtcctcaaa agttgcaccg
      241 aaagtataac aaaaatcacc tgcaactttt gggcaacctg ggcatccaaa taaaggcacg
      301 aaacacaaaa ccaatggagc caaatatgga caaacttttg aacgaacagg ttgttggtca
      361 gatcaagcag gcatttgccg ccatcaccaa ccctgtgcag atcctgttct ttggaagcaa
      421 ggataactgc gaatactgcg gcgaaaccgg gcagctggtc aaagagatca gcgagatcga
      481 tgaccgcgtt cacctctcca cacatgatct gcacgaagat gcggaactgg ccgcccaatt
      541 cagggtagat aaagcccccg tcatcgtcat tgcaggcagg gacggagata cggtcgttga
      601 cctgggaatt caattctcag gcattccctc cgggtatgaa ttcagcaccc tgatcaacga
      661 tatcctcttc gcttccaagc gggattccgg gctgggtgcg gcaacaaggg aatttctcaa
      721 atccctgacc aaacccctgc atttacaagt ctttgtcacc cctacctgac cgcattgtcc
      781 gcgagccgtg ttgctcgcgc accagatcgc catggaaaac ccaggcatgg tccatgcgga
      841 agctgtggat gccatggaat tccctgaact gacccagcgc ttccgtgtgc agggtgtgcc
      901 tcaaaccgtg atcaactccg gggaaggcat tgtcgtcggc gcatatcccg aacagcaatt
      961 gctggcagag atccgatctg cgcttggaaa ctaaagatcg caggaaaacc atgtcaaacg
     1021 cgaacaagca aaaatacccc ggtaaaagac aaaaacgccg caattcatcc actgcaatgg
     1081 ggatcgggtt gattctgttt ggggtcgcct tctttttgtg gctgttgggc agcagcggaa
     1141 aagcaagcct gccaaccgcc gatgaaatct ccattgtgcc tgccgaagtg aatttccctg
     1201 cgccgcaact cgcgctggaa aacctcaatg gtgaaaagca aaccctggaa gattatcaag
     1261 gccgtattgt ccttgtcaac aattgggcta catggtgccc accctgcaag gcagagatgc
     1321 ccatcttgaa acaatatcac gatacgcatg ccgctgaagg gttcaccgtc attgccgtgg
     1381 aggcaggcga tgcaaaagac accgtcgctg aattcgcaaa aagcctgggg ctgccctttc
     1441 ccgtctggct ggaccccgaa agcgcctcga tcaacgccat tcacaacggc agtctcccca
     1501 actcctacgt gatcgaccgc acaggcaccg tccgctatgc gtggacaggc gaagtgacca
     1561 tgacgatgct tgaaaaatac atcaccccgc tgatcgcgga aaacaattaa acaaaaaagg
     1621 agtggatttt atccactcct tttttgtttc aaacaatcag gcgcaagacc tgtttttatt
     1681 ttactttctc ttccacaacc ttcgctaatt taccgagcgc ttcattgacg ttgccatcag
     1741 gcagactgcc ttgtgcgaga ttgggacgcc cgccaccttt gccgccaatg cctgtgatga
     1801 ggtcaccagc tttgagtccg cgcttgacga ggtcttcggt caccactgcg atgacagttg
     1861 tgcctgttac gaggacagcc gctcccgctt tgggatattt ctcgcggaac ttgtcggcca
     1921 gggaacgcaa cgtgtctgta tcagcattga caatttccgc tgcgaggacg cttacatctt
     1981 tgacggtttg aacgtttgaa agctgcaggt tgaacgcgga aagagcagat tgtgtgcgca
     2041 ggttggcgag ttctttcttg agttcagaaa cttcatcctg caaggcatcc actttcgcag
     2101 gcacttcttc cacagcggcc ttcaaagagc cagccgcctg cttcaaggtt ttgaagcgct
     2161 tgtggatgag gtcgtacgcg ccgcggccag tgacagcttc gatacggcgc acccctgccg
     2221 ccgcagaact ttcactgacg atgataaatg cgccaacatc ggaagtgcgc tctaaatggg
     2281 tgccgccgca caactcatag gaatatttat cctcgccgcc ttccataatg gaaatggtgc
     2341 ggaccgtttc gccgtatttc tcgccgaaga gcgccattgc gccttctttc ttggcttcat
     2401 ccaaagactt ggtcaccttc actacgggca tatccattgc cacggcttca ttgaccatgc
     2461 gttccacacg ctccacctga tcgggggtca tggcttcggg gtggttgaag tcaaagcgca
     2521 aatgggttgg ggatacgagt gaacctgcct gcttggcctg atcactcaac acctcatgca
     2581 atgccttgtg cagcaagtgg gttgccgtat ggttgcgcat gatatcgtgg cggcgattcg
     2641 catccacttc tgcggtcgct ttgtcgccca ctttggggtg acccgagatc acctcaccaa
     2701 tatgcacgat cacacccgca gacgcgcgac ggatgccagt gacttcgatc tcccagcctt
     2761 cgccgcggat gaagcccgta tcattaacct gaccacctgc ttcgatataa aagccggtct
     2821 tgggcaaaat gacttcaact tgatcgtcga ggttggcaga gtccaccgat tgtccattga
     2881 ccacaagagc gagaatttca aggttggaat agcgggaaac atccagcccg ttgtaggggt
     2941 cgtattccac gccgtctttg cttaattttc cttttgcctg caaatctttc aggatgcctg
     3001 caaagaattc tgcatcttca ccgccgagtt tgcccatggc cttgccgccg ccagatgcaa
     3061 tggcatgttc ttccttcgca gtagtgaagc ccttctcatc cacgtccagg ccttgttcgc
     3121 gggcgatatc acgggagatc tcgaaaggca ggccgtaggt ggcgtacagg tcgaaggctt
     3181 tgtgaccatc gagaacggtc tgattggtcg agcggagttc ggagagcaga ttctctaaat
     3241 gggcagtacc cgcttcaacg gtccgtgcga agcggatttc ttcccgtgtc agattatcca
     3301 aaatagcggg ttgtgtcttg cgtaactcag gatagaagtc gccatattgt tcgatgacgg
     3361 cttcagcaac tttcgcgagg aagggttcgg tgagcccgat cttggtgcca aaccgcgcgg
     3421 cgcggcggac gatcatgcgg gctacataat tgcggcctgc attaccaggc acgacgccat
     3481 cggcgatgag gaaggaagcc gagcggacat ggtcacaaat aacgcgatag ggtgtgaaat
     3541 tcgcgagcat ttccttttcg gtgtgacccg tgagcgcgcg cagcacttca agcgaacctg
     3601 tgaaaaggtc agtcttgtag tttgagtcga caccctgtaa aacagaaacg atgcgctcga
     3661 agcccatgcc tgtatcaaca tgcttggcag ggagaagttc gaggcggcca tcatcaaaga
     3721 ggttgtattg aatgaaaacg ttgttccaca gttcgagata gcgggtgcat tcgccattca
     3781 caccgcagac gtggtcggtg ccgcgca
//
LOCUS       DAOQJC010000226         3571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_16554_length_3571_cov_12.206485, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000226 DAOQJC010000000
VERSION     DAOQJC010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16554_length_3571_cov_12.206485"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..874
                     /locus_tag="PLE39_19035"
     CDS             74..874
                     /locus_tag="PLE39_19035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE70486.1"
                     /translation="MNVNFGLTASDYAKHRAGFPDVFFERMFDEEIVKAGDVLADLGT
                     GTGTLARGFAERGCSVTGIDISAQLLEQAKDFALQAGLEIDFRFAKAEATGLPDSSFD
                     VVTAGQCWHWFDRPSAALEIKRILKPRGRVVIAHFDWLPLNGNVVDLTERLIETYNPK
                     WYVEFGGKTGVYPDWLRDLGEAGFLDIQTFSFDMDVPYTHEDWRGRIRASSGVGASLS
                     EAEVGRFDSELKSMLEGYEQAGAQSEAGSISVLQIPHRVFVVHAHKPN"
     gene            1228..2154
                     /locus_tag="PLE39_19040"
     CDS             1228..2154
                     /locus_tag="PLE39_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNE70487.1"
                     /translation="MLSIYKTTEQGLEKLETVANGTWVNVADPTPEEIEKLVAWGMDM
                     DYINYSLDQDEMARMERDEDYTFILLRIPMHQPESDVPFNTAPLGIMILGNKIITVCR
                     YDSDIFRVLANGKYRLLKTGKRYRLALYIFLETSARYLNLLREINRATEAVEDQLQKS
                     TRNREVLELLKYQKSLTYFATALRSNEVMMERVQKTQLFNYYEEDQDLLEDVLTENQQ
                     AIQMTSIATEILSGMMDAFASIISNNLNAVMKALAALTIILNMPSLVASFYGMNVALP
                     GEGHPLAFLTVIGLSLALTAIATYIFYKRDWF"
     gene            2156..3010
                     /locus_tag="PLE39_19045"
     CDS             2156..3010
                     /locus_tag="PLE39_19045"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNE70488.1"
                     /translation="MQTELLLDAKATLGEGPAWDAKTQTLYWLDILEKRVYADTRILA
                     QLDELVGCLAPRKNGNLLLGTRFSFADLAPDSAQLTVLATLSDAERPTNRMNDGKCDP
                     AGRFIAGTMDMNEKDPHGSLYSFDGKQTTRLLNGVTISNGLTWSVDHKTFYYIDTPTH
                     EVKAFDYDLASGQIANPRVAIRIPESLGWPDGMTSDTDGNLWIALWGGAQVTKWNPNT
                     GKLLEQIPVPALQSSACVFGGKNMNELYVTSARVGMSDANLQKYPLSGGLFKIVTGVT
                     GMPTFEFG"
     gene            complement(3062..3490)
                     /locus_tag="PLE39_19050"
     CDS             complement(3062..3490)
                     /locus_tag="PLE39_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70489.1"
                     /translation="MTVLPESLLEVREHHEPDYKPMVDYQSWRVALMNYTADLLPEKI
                     NRVQKHTETDEVFVLMKGRCILFIGEGEESVTKIHAVDMELYKLYNVKKGVWHSHTFS
                     EDARVLIVENRDTVVENSPFVPLSESQCKEVVELTNKLWG"
BASE COUNT          853 a          877 c          937 g          904 t
ORIGIN      
        1 attttcaagt ttcgaatttg tgagaggccg cttttgcagc ggtctttctt aatggaacgg
       61 agtcgattgc ttcatgaatg tcaactttgg gttaacagca agtgactatg ccaaacatcg
      121 tgcgggattt cccgatgtgt tctttgagcg gatgtttgac gaagagattg tcaaagcggg
      181 ggatgtgctt gcagacctgg gcacaggcac gggcaccctt gcacgcggat ttgccgaacg
      241 cggctgttcg gtgacgggaa ttgatatttc tgcacagctg ctggagcagg cgaaggattt
      301 tgccttgcag gcagggctgg agatcgattt tcgttttgcc aaggcagagg cgacaggttt
      361 acccgattcc tcttttgatg tggtgacggc ggggcagtgc tggcattggt tcgaccgccc
      421 atcagcggcg ctggagataa agcgcattct caaacccagg gggcgtgtgg tcattgcgca
      481 cttcgactgg cttccgctca atggcaatgt ggtggacctc acggaacgat tgatcgaaac
      541 ctataatcca aaatggtatg ttgaatttgg cggaaagacg ggtgtgtacc ccgattggct
      601 gcgcgatctt ggcgaagctg gttttcttga tattcaaacc ttttcattcg atatggatgt
      661 gccctacacc catgaagact ggcgcggacg catccgtgcg agctcggggg tgggggcgag
      721 cttgtcggag gcggaggttg ggagattcga ctcggagtta aagtcgatgc ttgaggggta
      781 tgagcaggcc ggggcgcaga gcgaagcggg ttcaatttcc gttttgcaga tcccgcatcg
      841 tgtttttgtt gtgcatgcac acaaaccaaa ttgattcgca aaaatagaga cttggaacgc
      901 gaatagcgcg aatgggcgaa tttcgcaaat tttcttggat ttattcgccc cattcgcaaa
      961 ttcgcggcat tcgtgttgag gttttcctga ctttgctaca gccatgaatt gattcgttgc
     1021 cacggttgtg caattcaatt aacgggcgta aaatagccca gcgaataacg gtaaggaaat
     1081 catgtatcac acggacgaga gtgacctaag ttcttctgac aataacccgc ccagtatagg
     1141 tgggcggctg atgtttaatt taattccaag catcacgtag gtgccatgcg cctctgcgtg
     1201 atgttaattc acgcaaggag gttgccaatg cttagcatct acaaaaccac ggagcagggg
     1261 ctcgaaaaac ttgaaacggt cgcgaacggc acctgggtta atgtggcaga cccaacgccg
     1321 gaagagatcg aaaaattggt cgcctggggg atggatatgg actatatcaa ttattcgttg
     1381 gatcaggatg aaatggcgcg tatggaaaga gatgaagatt acaccttcat ccttcttcgc
     1441 attcccatgc accagcctga aagcgatgtc ccctttaaca cagcgccgct tggaattatg
     1501 attttgggaa acaagatcat cacggtctgc cgttacgaca gcgatatctt tagagtgctg
     1561 gcaaatggta aatatcggtt gttgaaaacg ggcaaacggt accgcctggc tttgtacatc
     1621 ttcctcgaaa cctccgcgcg ttatttaaac ctgttgcgtg agatcaaccg cgccaccgag
     1681 gcagtggaag accagcttca aaaatccacg cgcaatcgtg aagtgttgga attgctcaaa
     1741 tatcaaaaga gcctcacgta tttcgccact gcgctgcgtt ccaacgaagt gatgatggag
     1801 cgcgtgcaaa aaacgcagtt gttcaattat tacgaagaag accaggacct gctcgaagat
     1861 gtgctgaccg aaaaccagca ggccattcaa atgaccagca ttgccactga gatcctttca
     1921 ggcatgatgg atgcctttgc ttcgatcatc tccaacaact tgaatgccgt catgaaggcg
     1981 ttggccgcgc tcaccatcat cctcaacatg ccttcccttg tggcttcatt ttacggaatg
     2041 aacgtggccc tgcccggcga aggccatccg ctggcattcc ttacggtgat cggtttgtcg
     2101 cttgcgctta ctgccattgc cacgtacatc ttctacaaac gggattggtt ctaacatgca
     2161 aactgagttg ctcctcgatg ccaaagccac gctgggtgaa ggccctgcct gggatgcaaa
     2221 aacgcaaacc ctttattggt tggatattct cgaaaaacgc gtttatgcgg atactcgaat
     2281 ccttgcccaa ttggatgaac ttgtcggatg ccttgccccg cgcaagaacg gaaacctgct
     2341 ccttggcacg cgcttcagct tcgccgacct tgcgcctgat tctgcccaac tgacagttct
     2401 tgccactttg agtgacgcag agcgtcccac caaccgcatg aacgacggca aatgtgatcc
     2461 tgctggccgt ttcatcgcag gcaccatgga catgaatgag aaggatccgc acgggtcgct
     2521 gtattccttc gatggaaaac aaacgacccg cctgctcaat ggtgtgacca tctccaacgg
     2581 cctcacatgg agcgttgacc ataaaacctt ttactacatc gatacgccca cacatgaggt
     2641 caaggctttt gattacgatc ttgcctctgg gcagatcgcg aacccgcggg ttgcgatccg
     2701 tatcccagag tcacttggct ggcccgatgg catgacctcc gacacagacg gcaatttgtg
     2761 gatcgctctt tggggtgggg cgcaggtgac aaaatggaat ccgaacacgg gcaaattgct
     2821 cgaacagatt cccgtccccg cattacaaag ctccgcatgc gtgttcggcg gcaaaaacat
     2881 gaacgagttg tacgtcacct cggcgcgggt ggggatgagc gatgccaatt tgcaaaaata
     2941 cccgctctcc ggcgggctgt tcaaaatcgt tacaggggtg acgggtatgc cgacgtttga
     3001 atttgggtag aaagaggaaa gaagaaagag gaaaggattt ttttcctctt tcttcataat
     3061 attatcccca aagtttgttt gttaactcca ccacctcttt acactgactt tccgacaatg
     3121 gcacgaatgg agaattttcc acgaccgtgt cacggttttc tacgatcaac actcgggcat
     3181 cttcgctgaa ggtatgcgaa tgccaaactc cctttttcac gttgtacagt ttgtatagct
     3241 ccatatccac cgcgtggatt ttggtcacac tttcctcccc ttcaccgata aataagatgc
     3301 agcggccttt catcaacacg aagacctcgt cggtttcagt gtgtttttgc acacggttga
     3361 tcttttccgg gagcaggtcg gcagtgtagt tcatcaatgc cacgcgccat gattggtaat
     3421 ctaccattgg cttgtaatct ggctcgtggt gctcgcggac ttcgagcagg gattcgggta
     3481 atacggtcat tggtctctcc atgtttggtc tctttcattt gacctcttgc ataagtaaag
     3541 tgttgtagac cacaaagtca ccaaggcact a
//
LOCUS       DAOQJC010000227         3543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_16751_length_3543_cov_6.950975, whole genome shotgun sequence.
ACCESSION   DAOQJC010000227 DAOQJC010000000
VERSION     DAOQJC010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16751_length_3543_cov_6.950975"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..455)
                     /locus_tag="PLE39_19055"
     CDS             complement(42..455)
                     /locus_tag="PLE39_19055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HNE70490.1"
                     /translation="MASVGYSGTPLAKKLGLKPTLTLLTIDAPKEYRSWLGELSGGVS
                     LVETAREPYEAVHVFATESAFLDGILSKLRRELKQDGFVWVSWPKKSSKVPTDITEDV
                     IREIALPLGFVDIKVCAVSEVWSGLKLVIRKSERS"
     gene            complement(455..1051)
                     /locus_tag="PLE39_19060"
     CDS             complement(455..1051)
                     /locus_tag="PLE39_19060"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNE70491.1"
                     /translation="MKIAIIGAGNIGGTLGRKWARTGHEVRFGVRNPADSKFDSLRSA
                     GAVVSVAEALVAADVVLLSMPGGAVADFAEQHGATLAGKIVIDSTNSVRNAHMNNLAA
                     LKEKSPEAKLIRAFNALGWENFEVPQINGVQIDLFYCGHAESRTITDQLIAEIGLRPI
                     YIGDLDLAAVLDGMTRMWFALALAQGRGRRIAFKLMEE"
     gene            1298..>3543
                     /gene="recJ"
                     /locus_tag="PLE39_19065"
     CDS             1298..>3543
                     /gene="recJ"
                     /locus_tag="PLE39_19065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNE70492.1"
                     /translation="MTSRWLDPQPVNTDPLHALGLPPLVAQTLLRRGISSPVEAEAFL
                     HPDATPPVSFPDIEKAVAAILVAIRKNDLICVWGDFDVDGQTSTALLVQTLQILKANV
                     TYYIPIRGRESHGVHIESLKPILDHGAKLLLTCDTGIAAHEAIEYANSRGVDVVVTDH
                     HDLGETLPNAKAIINPKLLPEDHPLKHLAGVGVAYKLAEALLAENHPSELANLLDLVA
                     LGLIADVALLKGETRALAQKGIEALRATNRLGLKVIAELSGTALETLTEETIGFTFAP
                     RLNALGRLGDANPAVELLLTKDPPRARVLSAQIEGLNAQRRLLTSQVYEAAEAKLREA
                     PDLLTEPAIVLSHPGWPGGVVGIVANRLVDRYHKPALLLTESEDGIVRGSARSVEGLH
                     ITEAIATQKDLLLGFGGHPMAAGLSMPLDKLADFRRGLGQAVEKQLGEIVFDEPTLQI
                     DAWLDLAEINISLADALEMLAPFGAGNPALTLATRGVTLKSVTTIGKTKEHLRLTIED
                     GAGKTQSILWWGGAGEELPPTDNKFDIAYSLRASTFRGQKQVTLQFEEFRVVEEKPIE
                     ARKTKVEIRDMRLQSSLLNLQPSTLIWAEGADRAVGTPRFELRPADEFSIYTTPASPA
                     DLRKALGIVKPKIVYVFAIPPAEEKPEEFLTHLAGLCKFTLNQRRGKVTVRELAAAMA
                     TRESAIQIGLEWMSAGGQLSVAFDEDQITLSAEKQEKNPYLQAELFVALKGILNETAA
                     YRKYFASA"
BASE COUNT          844 a         1037 c          913 g          749 t
ORIGIN      
        1 cgcctacatg aaggtgcaag ccaactagcc gaatacggat gtcaactccg ctctgattta
       61 cgaatcacca atttcaaccc tgaccatact tcactcacgg cgcacacctt gatatccaca
      121 aacccaagcg gcagtgcgat ctcgcggatg acgtcttcgg tgatatccgt cggcactttg
      181 gatgatttct tcggccagga aacccacacg aaaccatcct gcttcaattc cctgcgcagt
      241 ttggacaata tcccatccag aaacgcgctt tccgtggcaa agacatgaac tgcctcatac
      301 ggctctctgg ccgtttcaac caacgatacc ccgcccgata attctcccaa ccatgaacga
      361 tattccttcg gcgcatcgat cgtcaaaagg gtcagtgtgg gcttgaggcc gagtttcttt
      421 gcaagcggag ttccagaata accgacagat gccattactc ctccattaat ttgaaagcga
      481 ttctgcgtcc acggccctgg gcgagggcga gggcaaacca catgcgggtc atgccatcca
      541 gcacggcggc aaggtcaagg tcgccgatgt agatcgggcg caggccaatc tctgcgatca
      601 attgatcggt gattgtccgc gactctgcat ggccgcagta gaacaagtca atttgcacgc
      661 cgttgatttg cggcacttca aaattttccc accccagggc gttgaacgcc cgaatcaatt
      721 ttgcttcagg tgacttctct ttcaaggcgg ccagattgtt catgtgcgca ttgcgaacgc
      781 tgttggtcga gtcgatcaca attttccctg caagggttgc gccgtgctgc tcggcaaaat
      841 ctgccaccgc accgccaggc atggaaagca ggacgacatc cgccgccacc aatgcttccg
      901 ccacagacac caccgctccc gcagaccgca gggagtcaaa ttttgagtca gcggggttcc
      961 gcaccccaaa gcggacctca tgcccagtcc gtgcccactt ccttccaagc gtgccgccga
     1021 tgttccccgc accaatgatt gcaattttca ttttcagttc ctctccagat aaaagagctg
     1081 catcaatgat aacgcatgct gaaaaatctg atatgcgcca caagtaaggg ggcctgcgag
     1141 agaatccttt gtcgaggatg gtggaggaaa ctcacataca ggctccagcc accgtcctcg
     1201 gcggcgattt gcgaggcgcg aaggatacgt acgcaacgtg aacgttgagg tacaaaggcg
     1261 cacacaaaat catgacaagc gataaaatca attcatcatg acatcccgct ggcttgaccc
     1321 tcaacctgtc aacaccgatc cactccatgc gttgggcctg cccccgcttg tcgcgcagac
     1381 tctgcttcga cgcgggatca gctctcccgt agaagcagaa gcgtttcttc atcccgatgc
     1441 aaccccacct gtatcttttc cagatatcga aaaagcagtc gccgcaattc ttgtggctat
     1501 acgaaaaaat gatttgattt gtgtgtgggg tgatttcgat gtggatgggc aaacctccac
     1561 tgccctactg gtgcaaaccc tgcaaatttt gaaagcgaat gtgacctact acattccgat
     1621 ccgcggcagg gaaagccacg gcgttcacat cgaaagcctc aagcccattc tcgatcatgg
     1681 cgcgaagttg ctgctcactt gcgatacggg cattgccgca cacgaagcca ttgagtatgc
     1741 caattcacgc ggcgtggatg tggttgtgac cgatcatcat gacctcggcg aaacgcttcc
     1801 aaatgcaaag gccatcatca acccgaaact gctgccagag gatcatccgt tgaaacatct
     1861 tgcaggtgtg ggcgtggcat acaaactggc tgaagccctg cttgctgaaa atcatccctc
     1921 tgaacttgcg aatcttctcg acctcgtggc gctcggcctc atcgccgatg tggcactgct
     1981 taagggtgaa acacgcgcgc ttgcgcaaaa agggatcgaa gcactgcggg caaccaatcg
     2041 gttggggttg aaggtcattg cagaactttc aggcaccgca ctcgaaacgc tgaccgagga
     2101 aaccattggc tttacatttg ctccgcgctt gaatgccctg gggcgtttgg gcgatgccaa
     2161 ccctgcagtt gaattgctgc tcacgaaaga tccgccgcgc gcacgtgtgc tatctgcaca
     2221 gatcgaaggc ctcaacgcac agcggcggct gttgacgagt caggtgtatg aagcggcgga
     2281 agcaaaattg cgcgaggctc ccgacctgtt gaccgagcca gccattgtgc tctctcaccc
     2341 tggctggcct ggtggtgtgg tgggcattgt ggccaatcgg cttgtagacc gttatcacaa
     2401 acccgcattg cttctgaccg aatcagaaga cggcatcgtg cgcggctccg ctcgctcggt
     2461 ggaaggtctg cacatcaccg aagccatcgc cactcaaaaa gacctgctgc tcggtttcgg
     2521 cgggcaccca atggcagcgg gcctttccat gccattggat aaacttgcag actttcgacg
     2581 tggacttggt caggcggtgg agaaacaact gggagagatc gtcttcgatg aacccacgct
     2641 tcagatcgac gcctggcttg atctggcgga aatcaacatt tccctcgccg acgcattgga
     2701 aatgctcgcc cccttcggtg cggggaatcc cgcgctcact ttggcaaccc gcggcgtaac
     2761 cttgaaatct gtcaccacca tcggaaagac caaagaacat ctgcgcctga cgatcgaaga
     2821 tggggcaggc aaaacacaaa gcattttgtg gtggggcggc gcaggcgaag aactgccacc
     2881 caccgacaat aaatttgaca ttgcctattc cctgcgtgcc tccacattcc gcgggcagaa
     2941 gcaggtcact ttacagttcg aagagtttcg tgtggtggaa gaaaaaccca tcgaggcgcg
     3001 aaaaaccaag gtcgagatca gagacatgcg cctgcagtca tccctgctta atttgcagcc
     3061 atccactctc atctgggccg aaggcgccga cagggctgtc ggcactccac gctttgagct
     3121 gcgccccgcc gatgaattct cgatctacac cacacccgcc tcccccgccg atttgcgcaa
     3181 agcattgggc attgtcaaac caaagatcgt ttatgtgttt gccattccgc ccgccgaaga
     3241 aaagccagag gaatttctaa cccaccttgc aggcctgtgt aaattcacgc tcaaccagcg
     3301 ccgcggcaag gtgaccgtgc gcgaactggc cgcagcaatg gcgactcgcg aaagcgcgat
     3361 ccagatcggg ttggagtgga tgtcggcggg aggtcaactc tcggtggcgt ttgacgaaga
     3421 ccagatcacc ctgtctgctg aaaagcagga gaaaaatcca tacctgcagg cggaattgtt
     3481 cgtggcactg aaaggcattc tcaacgaaac cgccgcttac cgaaaatatt ttgcaagcgc
     3541 cga
//
LOCUS       DAOQJC010000228         3460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_17437_length_3460_cov_7.896916, whole genome shotgun sequence.
ACCESSION   DAOQJC010000228 DAOQJC010000000
VERSION     DAOQJC010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17437_length_3460_cov_7.896916"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="PLE39_19070"
     CDS             complement(<1..1165)
                     /locus_tag="PLE39_19070"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE70493.1"
                     /translation="MIQNPEEAVSLVEVVRFYADRIGNELAYMYLVDGEDQEAPLTFS
                     ELDRQARAFAATLQQHTKPGDRALLLYPSGLEFIVAFFGCLYAGVVAVPTIVPHLKRA
                     TPRFRAMLSDADAVVACTQMELFDKLKQLFDEYPEFHDLKWVVNEKLEAGVESQWQEV
                     ENKADDLAFFQYTSGSTSTPKGVMITHFNLIETVRDLQRGSAFDDKSAVASWLPIFHD
                     LGLIFGLLMPVYLGRTSYFMAPIAFLERPYRWLKAISKYKATHSAAPNFAYDLCVQKV
                     TNEEKETLDLGGLKIIGNAAEPVRFETMQMFSDAFKSCGFDFNLFAPAYGLAEATVKV
                     SGRLYGQKISYCLLDSDALAKHKLVFLSEEGPKSYVSVGCGWSSINADIRIVNA"
     gene            complement(1162..1413)
                     /locus_tag="PLE39_19075"
     CDS             complement(1162..1413)
                     /locus_tag="PLE39_19075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNE70494.1"
                     /translation="MEEIEIKEKLKKFICTEIMNDADYPLKFDEPLITSGLIDSFSLA
                     QVGVFVEIEFQIYIPDPELTVDNMNTIDAMAARIKEKMG"
     gene            complement(1415..2869)
                     /locus_tag="PLE39_19080"
     CDS             complement(1415..2869)
                     /locus_tag="PLE39_19080"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE70495.1"
                     /translation="MTDLFSNLSITSNGFFFFVLAALGVYYLVPKKAQNHVLLIASYL
                     FCFTWDWTFAAILLASSIVNYWVALKLDKAEVTAKTRWVWIGVAANLGVLLFYKYFDQ
                     TSVYFFKALLRIGLTNELLSLDLLQPIGISFYTLQAISYLVDVYRKQIPANHSFVEVS
                     LYLGYFPKLLAGPIERAQNFFKQLQADVEIGNDQLVESFIKIFVGLVRKLVISETIVR
                     MIPLGVFAAPQKYSMPDLLFWWLTFAFVIYNDFAGYTSIARGVSGLFGIQLSPNFEQP
                     FFSTSYIDFWNRWHISLSNWLRDYIYLPVSRAMLRRNPSGRYWPNLVVPPLVTMIVSG
                     IWHGAAPHFILWGALNGLLQGYERVQKTFVKKTTVQPPRWRRAVSISVTLLVLILINA
                     PFKMDFSQTLAFWSGLFRWGIIQTLSVHQIIKPILAIGLSFFIDVMQLPAKDELGLLR
                     LSKPVKVFLLTVGLLLLFLSTRQAAVAPFIYQEF"
     gene            complement(2937..>3460)
                     /locus_tag="PLE39_19085"
     CDS             complement(2937..>3460)
                     /locus_tag="PLE39_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70496.1"
                     /translation="WLIENSNSYRFYLGADRYLLNGEENADHIGLNGHSLKFSEKTNM
                     SQQEQVDYFDSLLDRPEITGEQLDGLHELLALNSAETKIVLFETPVDPIFFTLQKFAY
                     NRKVYPDFRNMLENEAAYVNTDLWLTQESIQIPMDGWYDIVHFNKVGSEYFSRLLGEH
                     LSSIQIPISGQIP"
BASE COUNT          927 a          867 c          879 g          787 t
ORIGIN      
        1 gcgcgttcac gattcggata tctgcattaa tggaactcca tccgcagccc acgctaacat
       61 agcttttggg gccttcttct gaaaggaata caagcttgtg tttcgcaagc gcatctgaat
      121 ccagcaagca ataagatatc ttttgaccat acagccttcc gctcaccttt acagttgcct
      181 ccgcaagacc atacgcaggc gcgaacaggt tgaaatcaaa gccacaggac ttgaaagcat
      241 cactgaacat ctgcatggtt tcaaatctga ctggttctgc ggcattgcca atgatcttca
      301 aaccaccaag gtcaagggtt tctttttcct cgttcgtcac cttttgcaca cacaggtcgt
      361 aagcaaaatt tggagccgcg gaatgagtag ccttgtattt tgagatggcc ttcaaccacc
      421 gataaggtct ctccaaaaat gcgatcggtg ccataaagta gcttgttcta cccaaataca
      481 cgggcatcaa caagccaaag atcaggccaa gatcatgaaa gatcggcagc caggaagcga
      541 ctgcgctttt gtcgtcgaaa gccgagcctc gctgcaaatc acggactgtc tctatcaggt
      601 tgaagtgtgt gatcatcaca cccttgggcg tgctggtgga gccagaggtg tattgaaaga
      661 aagccaggtc atcggctttg ttttcaactt cctgccattg cgattcaaca cccgcttcga
      721 gcttttcatt gaccacccat ttcaggtcat ggaattcggg atattcatcg aataactgtt
      781 tgagcttatc gaacaattcc atctgtgtgc aggccactac agcatcggca tcacttagca
      841 tggccctgaa cctgggcgtg gcacgtttca ggtgcggaac aatggtcggc acagcgacga
      901 cgcccgcata caagcacccg aaaaaagcga cgatgaattc cagacctgat gggtacagca
      961 acaaggcgcg gtcacctggt ttggtgtgct gctgcaaagt ggcggcaaat gcccgtgcct
     1021 ggcgatcaag ctcggagaag gtcagaggcg cttcctgatc ctccccatcg acaaggtaca
     1081 tataggccag ttcattcccg attcgatccg cataaaagcg gaccacctcc acaagactga
     1141 cagcctcttc agggttttgg atcatcccat tttctccttg atcctggcgg ccatggcatc
     1201 gatggtgttc atgttgtcga ccgtcaactc agggtcggga atatagatct ggaactcgat
     1261 ctcgacaaac accccaacct gcgcgagcga aaacgagtcg atcaacccgc tggtgatcag
     1321 cggctcatca aacttaagcg gataatcggc atcgttcatg atctcggtgc agatgaattt
     1381 tttcagtttt tccttgatct cgatttcttc catgttagaa ctcctgataa atgaatggag
     1441 ccactgccgc ctgccgcgtg gacaggaaca aaagcagaag ccctaccgta agcaagaaca
     1501 cctttacagg ttttgacaga cgcagcaaac ccaattcgtc tttggctggc aactgcatca
     1561 cgtcaatgaa aaaggacaag ccgattgcca gaatgggctt gatgatctga tgaacggaca
     1621 gcgtctgaat gataccccat ctgaacaggc cgctccaaaa tgccagtgtc tggctgaaat
     1681 ccattttgaa aggggcgttg atcaggatca acacaagcag agtcactgaa atgctgacag
     1741 cccgtctcca gcgcggcggc tgcacagtgg tctttttgac aaacgttttt tgcacacgtt
     1801 catatccctg cagcaaaccg ttgagcgcac cccacaaaat gaaatgcgga gccgccccat
     1861 gccagatgcc gctgacgatc atcgtgacca gcggcgggac gaccagattg ggccaatatc
     1921 gtccactggg attgcggcgc agcatggcac ggctgacggg caggtaaatg taatcacgca
     1981 gccagttgga aagggaaata tgccagcgat tccaaaagtc aatgtagctg gtggagaaaa
     2041 acggctgctc aaaattcggt gaaagctgaa tcccgaacaa tccgctgact ccccttgcga
     2101 tgctggtgta acctgcaaaa tcattgtaga tgacgaaggc aaaggtcagc caccagaaaa
     2161 gcagatcggg catggaatat ttttgcggag ccgcaaatac acccagggga atcatccgca
     2221 cgatggtttc agagatcacc aatttacgga cgaggccgac gaagattttg ataaaacttt
     2281 caacaagctg gtcgttccca atttcaacat cggcctgcaa ttgcttgaag aagttttgtg
     2341 cccgttcaat gggacccgcc agcagtttgg gaaaatagcc gagatacagc gagacttcca
     2401 caaaactatg atttgcaggg atttgcttgc ggtacacatc caccaaatac gaaatggcct
     2461 gcagggtata aaacgaaatg ccgatgggtt gcagcaggtc aagggagagc aattcgtttg
     2521 tcagaccgat ccgcagcaga gccttgaaaa aatagaccga ggtctggtcg aagtatttgt
     2581 aaaacagcaa aacgcctaga ttcgcagcaa cgccgatcca cacccaacga gtcttggcgg
     2641 tgacttctgc tttatccagt ttgagcgcaa cccaataatt gacaatggac gaggccagga
     2701 gaatggcggc aaaggtccag tcccaggtga agcagaagag atacgaggcg atcagcagca
     2761 catggttttg cgctttttta gggacgaggt aatagacccc aagcgcagcc aggacaaaga
     2821 aaaagaaacc gttggaggtg atggaaagat tcgaaaaaag gtcagtcatt gggttattca
     2881 ttgctcaagc cgctgtccca gcagcgagga cgccgcggga gcatagcatg agggcatcaa
     2941 ggtatttgcc ctgaaatggg aatttgaatg ctgctcaaat gctcgccaag caggcggctg
     3001 aaatattcgc tcccaacttt gttgaaatgg acaatatcgt accagccatc catcgggatc
     3061 tgaatagact cctgtgtcag ccacagatca gtgttgacat aggctgcttc attttccagc
     3121 atgttgcgga aatcgggata caccttgcgg ttgtaggcga atttttggag ggtgaaaaag
     3181 atggggtcaa caggtgtttc gaacaggacg attttcgtct ccgcgctgtt cagggccagc
     3241 aattcgtgca agccatcgag ttgttcgcca gtaatttctg gtctgtccaa cagactgtca
     3301 aagtaatcca cctgctcctg ctgggacatg ttcgtctttt cagagaattt cagggagtga
     3361 ccgttgagcc cgatgtggtc tgcgttctct tcgccgttga gcaggtaccg atccgcgccg
     3421 aggtagaagc gataggaatt cgagttttcg atgagccagc
//
LOCUS       DAOQJC010000229         3405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_17900_length_3405_cov_10.524776, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000229 DAOQJC010000000
VERSION     DAOQJC010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17900_length_3405_cov_10.524776"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1167)
                     /gene="hypE"
                     /locus_tag="PLE39_19090"
     CDS             complement(112..1167)
                     /gene="hypE"
                     /locus_tag="PLE39_19090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNE70497.1"
                     /translation="MENEIDFEGYTCPVPVQPRDTVVLGHGSGGKLSHDLINRLFLPD
                     LGKAAPRALDDAAVLNINGQRLALTTDSHVVSPLFFPGGDIGRLAVCGTVNDLAMVGA
                     RPIALTCGFVVEEGLSFEILQQVVRSMRESAAEAGVYIAAGDTKVVQKGGADKLFINT
                     SGVGVVEADVNISGANAQIGDVLIMSGTMGDHGVAVLSARENLQFETDLQSDVAPLNH
                     LVAVMMVAGEIHVLRDPTRGGVATTLNEIASQSKVGIELLETALPVKPQVRAACEMLG
                     FDPLNIANEGKLLAIVKAADAESILSAMRKTKYGEEACIIGKVIETKSPQVRLRTAIG
                     GTRLIDMLPGEMLPRIC"
     gene            complement(1183..2268)
                     /gene="hypD"
                     /locus_tag="PLE39_19095"
     CDS             complement(1183..2268)
                     /gene="hypD"
                     /locus_tag="PLE39_19095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNE70498.1"
                     /translation="MKYIDEYRDPAKVRGLLAEIGETVTQPWVLMEICGGQTHAFLHH
                     GLDEMLPGGIELVHGPGCPVCVTPLEQIDKALAIAARPEVIFTSYGDMLRVPGSSVDL
                     FNVRAKGGNVKVVYSPLEALKLAQENPEKEIVFFAIGFETTAPPNAMAVLQAKQKGIK
                     NFSVLVSHVCVPPAMEAILGSKHNRVQAFLAAGHVCAVMGYHEYPAIAEKYKVPIVVT
                     GFEPVDLLRGVLMAVKQLESGKAEVENAYERAVTFEGNQAAQQVVQTVFQPADRKWRG
                     IGLIPQSGWGLRPDFAEFDAEARFSVGEIITQESPLCIAGEILQGMKKPPQCPAYGKQ
                     CTPSSPLGAPMVSAEGACAAYYRYHRE"
     gene            complement(2265..2513)
                     /locus_tag="PLE39_19100"
     CDS             complement(2265..2513)
                     /locus_tag="PLE39_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNE70499.1"
                     /translation="MCLGIPGKLQKIYDEGSLKMGKVDFGGIVKEACLAYVPEVQVGQ
                     YVLIHVGFAISVLDEEEAQARLQMIHEIDALGEELGSV"
     gene            complement(2572..>3405)
                     /locus_tag="PLE39_19105"
     CDS             complement(2572..>3405)
                     /locus_tag="PLE39_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70500.1"
                     /translation="RVPVQHHHAHIAACMADNRLTDRRVIGLSFDGTGYGTDGAIWGG
                     EVLLASYADFERVAHLQYLPLPGSDSATRHPWRIAVGYAEALGLEINDLPFMQGLDAT
                     AVEIVRQQVRKDLNTPHTSSMGRLFDAVASLGGVRSDVSYEAQAAIELEVLSKRFING
                     AEAYPYQIRNGIVLLGELLQDIIADIRSKQPVGLIGARFHQTLCALAVEVCRNTRNET
                     GINEVALSGGVWQNQTLLNLTREKLADERFVVFSHRQTPANDGGLALGQAVIANYSSE
                     R"
BASE COUNT          779 a         1062 c          785 g          779 t
ORIGIN      
        1 ataactaccc gcttcacctc ggaccctgga tgctgggcgg cttcgttccg ctttttcttg
       61 gcctcggcct ggtcctgctt cactacctga ccgagaagga ataatccact actagcaaat
      121 cctcggcagc atttcacctg gcagcatatc aattaatcgg gttcccccaa tggcggtccg
      181 caatctcact tgcggacttt ttgtttcgat caccttccca atgatgcaag cctcttcgcc
      241 atattttgtc ttccgcatgg cagagaggat ggattccgca tctgctgctt tgacaatggc
      301 cagcagtttg ccttcgttgg caatattcaa cgggtcgaag ccgagcattt cacaagccgc
      361 ccgcacctgc ggtttgacag gtaatgccgt ttccagcaat tcgatcccca ctttcgattg
      421 tgaagcgatt tcattcaagg tagtggccac tccaccgcga gtggggtcac gcagcacatg
      481 gatttcaccc gctaccatca tcaccgcgac caaatggttc aacggcgcaa catccgattg
      541 caggtcggtt tcaaattgaa gattctcgcg ggccgataaa accgccaccc catgatcgcc
      601 catggtccca gacatgatga ggacatcccc gatctgtgca ttcgcgccag aaatatttac
      661 atccgcctcc accacgccca cccctgaagt gttgatgaag agtttatctg cgccgccttt
      721 ttgcaccacc tttgtatcgc cagccgcaat atacacaccc gcctccgccg ccgattcgcg
      781 catggacctg accacttgtt gcagaatctc gaaagacagg ccttcttcaa ctacaaatcc
      841 gcaggtgagg gcaatgggtc tggcacccac catcgccaga tcgttgaccg taccgcaaac
      901 cgccagcctg ccgatatccc cccctgggaa gaacaacgga gaaacgacat gcgaatccgt
      961 tgtcagtgcc agccgctgcc cgttgatgtt caacacagcc gcatcgtcca gtgcgcgagg
     1021 cgcggccttt ccaagatcag gcagaaagag tcggttgatc aagtcgtggc ttagttttcc
     1081 accggagcca tgtcccagca ccacggtatc ccgaggttga acaggcacag ggcaggtata
     1141 gccttcaaaa tcaatttcgt tttccatcaa taacctcttc acctattccc gatgataccg
     1201 ataataggcc gcacaagccc cttctgctga aaccatcggc gcgcccagcg gagaggatgg
     1261 tgtgcattgt tttccataag caggacattg cggcggcttc ttcatacctt gcaggatctc
     1321 ccctgcaata cataaaggcg attcctgtgt gatgatctca cccacagaga aacgcgcttc
     1381 ggcatcgaac tcggcaaagt cggggcgcag cccccagccg ctttgcggaa ttaaaccgat
     1441 gccgcgccac tttcggtccg caggttgaaa aacggtctgc accacctgtt gagcagcttg
     1501 atttccctca aaggttacgg cacgctcgta ggcattctcc acttctgcct tgcctgactc
     1561 caattgcttc actgccatga gcacaccgcg caacaggtcc actggctcga acccggtgac
     1621 cacgatcggc accttgtatt tttctgcgat cgcaggatat tcgtgatacc ccatcaccgc
     1681 gcagacgtgc ccagccgcga gaaaagcctg cacacggttg tgctttgaac ccaaaatcgc
     1741 ctccattgca ggcggcacac agacgtgtga aacgagcacc gagaaattct taattccctt
     1801 ttgtttggcc tgcaacacag ccattgcatt gggcggcgcc gtggtctcaa aaccgatcgc
     1861 aaaaaagacg atctcttttt cggggttttc ctgcgcaagt ttcaacgcct ccagcggcga
     1921 gtacaccacc tttacattcc caccttttgc acgcacattg aacagatcca cactggagcc
     1981 cggtacacgc aacatatcgc catacgaagt gaagatcacc tcaggacggg cagcaatcgc
     2041 caacgctttg tcgatctgct ccagcggggt cacacagact gggcagcccg ggccatgcac
     2101 caattcaatg ccgccaggca gcatctcatc caacccatga tgcaggaagg cgtgcgtttg
     2161 tccgccgcag atctccatca acacccatgg ctgggtgaca gtttcgccga tctctgccaa
     2221 taatccgcgc acttttgcag ggtcgcggta ttcatcaata tatttcatac actcccaagt
     2281 tcctccccca atgcatcgat ctcatggatc atctgtagcc tggcttgcgc ctcctcctca
     2341 tcaagcacac tgatcgcaaa gccgacatgg atcaacacat actgccccac ctgaacctca
     2401 ggaacatacg caaggcaggc ttcctttacg ataccgccaa agtccacttt gcccattttg
     2461 agcgaaccct catcatagat tttttgcagc ttccccggaa tgccaagaca cataacgaac
     2521 tccttattga aaaatcctgc taccttttga aacacatcgt ttctggtctg tctatctttc
     2581 agacgagtaa tttgcgatca cggcctgccc caatgccaga cctccatcgt ttgcaggtgt
     2641 ttgccgatgc gagaacacaa caaaacgttc atccgccaat ttctcacgcg tcaagttcag
     2701 cagggtttgg ttttgccaaa caccgcctga aagcgccact tcattgattc ctgtttcatt
     2761 gcgcgtgttc ctgcaaactt caaccgccaa ggcacacaga gtctgatgaa accgcgcacc
     2821 gatcaaccct acaggttgtt ttgacctgat atctgcgatg atgtcttgaa gcagttcgcc
     2881 caacaacacg atcccgttcc tgatctgata tggataggcc tctgccccgt tgatgaatcg
     2941 cttcgaaaga acttccaact cgattgcggc ttgtgcttcg taactgacat cgcttcgcac
     3001 cccgcccagg ctggctactg cgtcgaacag ccgacccatg ctcgaagtat gcggcgtgtt
     3061 caaatccttg cgcacctgct gacggacgat ctctacagcg gtcgcatcca gtccctgcat
     3121 gaagggcagg tcgttgatct ccagcccaag ggcttctgca tatccaacag caatgcgcca
     3181 cgggtggcgg gtggcagagt cactgccggg gagtgggaga tattgaaggt gtgcaacccg
     3241 ctcaaagtcc gcgtaggaag cgagcaatac ttccccgccc cagatcgccc cgtcggttcc
     3301 atagcccgtt ccatcgaaag acaatccgat cactctgcgg tctgtgagcc tgttatcggc
     3361 catgcaagcg gcaatgtgcg catgatgatg ttggacaggc acccg
//
LOCUS       DAOQJC010000230         3370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_18192_length_3370_cov_9.752036, whole genome shotgun sequence.
ACCESSION   DAOQJC010000230 DAOQJC010000000
VERSION     DAOQJC010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18192_length_3370_cov_9.752036"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="PLE39_19110"
     CDS             complement(<1..1164)
                     /locus_tag="PLE39_19110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE70501.1"
                     /translation="MDEKTVPIGNLPPHLMALLNKQKKVVDWGEDIHYRALFDQTGEV
                     VFIIGMDLHYITANRQALDLLGYKKEEIVGLPVSSVMSLSDGVEDGTVLDDDTNMVER
                     ILKRKDGSTFPVEVSASIIYNERNEPAYIQSIARDISERKKSELALTRYSRILSAINH
                     ATAQLLRSSKIGEMIPDALAALGEAIGVTGCAIFEVNTFSPQPAVLTQYQWIKNDTPD
                     LNVSDLILPIIPSILKASGGQFFNNDVGRHGPSFFVFPIYGTLDSWKFLGVFDTSKLL
                     AWSSSECDAVEMAAGLMGSALQRTSYEETIRENENRNRIILSVLPDLLIRLTTDGVIL
                     DYTTNPDHPLYLHRDMISGRRLSDIWPEETVQSILTVPNVDEDDEEQRPVFGFT"
     gene            complement(1157..2293)
                     /locus_tag="PLE39_19115"
     CDS             complement(1157..2293)
                     /locus_tag="PLE39_19115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNE70502.1"
                     /translation="MNSKPLILIIDDDPALLMGVSATIKKYGFQVVSAEDGNDGLAKA
                     KEIKPDLILSDVMMPPPDGLELRRIMSQDPQLASIPFIFLTARSGVEDKVSAIMDGAD
                     DYITKPFVVYELIARISAVLRRVKIAEENGREQARVAAEADLERVRQEILQNFHHEIR
                     TPLSNIMMPLQLAINNKFDDAQEQSRFLSKALSSASRLESLTTDLILLSNIDQGNLNT
                     IRQPIDMEMDIASIVKRRLDRYDEKGLDFTMDISSSGKISAPRREFTHALVHLVDNAL
                     KFTSKNGKVHLRITPTGNGGATLDVFDNGPGIPSRLHEKVFERFYQISQGDAREFEGM
                     GVGLSIARAVFRSLRGEVKILESLRGCHIQAHLPDPQPGDVTYG"
     gene            complement(2296..2670)
                     /locus_tag="PLE39_19120"
     CDS             complement(2296..2670)
                     /locus_tag="PLE39_19120"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNE70503.1"
                     /translation="MTQEEDNNLIPSREQNVFLIINRQMIPLEKKVTRLGRQLGNDVV
                     LQEESVSRFHAEIRYEDGKFILYDNNSTAGTYVNSKRIDRCVLISSDLISFSCIQVMF
                     VNNNAKLAGKAMGMTQKLHSRK"
     gene            2988..>3370
                     /locus_tag="PLE39_19125"
     CDS             2988..>3370
                     /locus_tag="PLE39_19125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE70504.1"
                     /translation="MFTIDTQLSNGGAKTHLILVADDNTMVLQMLTDFLEMQGYQVFQ
                     ARSGVELLEKISETHPDLVLVDIQMPRMDGLETIRRVRASQDGQISSIPLIAVTALAM
                     PGDRERCMLAGATEYVSKPLKLNELS"
BASE COUNT          920 a          826 c          773 g          851 t
ORIGIN      
        1 ggtaaaacca aacaccgggc gttgctcttc atcatcttca tccacatttg gaacggtcag
       61 aatgctttgc acggtttctt cgggccagat atcgctcaaa cgcctgcctg agatcatgtc
      121 acgatgcagg tagagcggat gatcggggtt cgtagtgtaa tcaaggatga ccccatcggt
      181 ggtcaggcgg atcaacagat caggcagaac cgaaaggatg atgcggttcc gattttcatt
      241 ttctcgaatc gtctcttcat aactggtgcg ctgcaaggcc gagcccatca accctgcggc
      301 catttcaacc gcatcacact cggaagaaga ccaggcaagc aatttggaag tatcaaaaac
      361 gccaaggaat ttccacgaat cgagtgttcc ataaatgggg aaaacaaaaa aagatgggcc
      421 gtgtctaccg acgtcattat tgaaaaattg tccacccgaa gccttcaaaa tggagggaat
      481 gattggcaga atgagatccg aaacattcaa gtctggggtg tcattcttga tccattgata
      541 ctgtgtgagg accgcaggct gcggcgaaaa agtgttgacc tcaaaaatag cgcaacccgt
      601 tacaccgatc gcttcgccaa gcgctgccag ggcatcaggg atcatttctc cgatctttga
      661 ggaacgcagc aactgggcgg tggcatgatt gatggcggaa agaatgcgcg aatatctggt
      721 cagcgccagc tcagatttct tcctctcgga aatatcccgc gcaatgcttt gaatgtaggc
      781 aggctcattt cgttcgttgt agatgatgga ggcgctcacc tcgacaggaa aagtggagcc
      841 atccttacgc ttgaggatgc gctccaccat atttgtgtca tcgtccaaca cagtgccatc
      901 ttccacccca tcactcaacg acatgaccga actgaccggt aagccgacga tctcttcttt
      961 tttgtatcca agcaggtcaa gcgcctggcg attggcagtg atgtaatgca ggtccatgcc
     1021 aatgataaag acaacctcac ccgtctgatc gaacaaggca cgatagtgaa tgtcttcgcc
     1081 ccaatccact acttttttct gcttgttcag caaggccatc aaatgcggag gtagattccc
     1141 tatcgggacg gttttttcat ccataggtca cgtcaccagg ttgagggtcg ggaagatgcg
     1201 cctgtatatg acatccacgc aatgattcaa gtattttcac ttcgccacgc aggcttctaa
     1261 atacagcacg tgcaatcgaa agtcccacac ccatcccttc aaattcacgg gcatcaccct
     1321 ggctgatctg ataaaaacgc tcgaacacct tttcatgcaa acgtgatggg atcccgggac
     1381 cattatcaaa aacatccagc gttgcgccgc cgttcccagt gggggttatg cgtagatgca
     1441 cctttccatt tttagatgtg aacttgaggg cattatccac cagatgaaca agggcatgcg
     1501 taaactcgcg acggggcgct gaaatttttc cgcttgaaga aatatccatt gtaaaatcca
     1561 ggcctttttc gtcgtaacga tcgaggcgcc ttttgacaat ggaggcgata tccatttcca
     1621 tatcaatggg ctggcgaatg gtattcaagt ttccctgatc gatattactt aataagatca
     1681 aatccgtagt aagcgattca agtcggctgg cgcttgagag ggccttgctt aagaaccgac
     1741 tctgctcctg ggcatcatcg aacttgttat tgatcgccaa ttgcagcggc atcatgatgt
     1801 tcgaaagcgg cgtgcggatc tcatgatgga aattctgcaa aatctcctga cgcactcttt
     1861 caagatctgc ttcggccgct acacgggcct gttccctacc attctcttca gcgatcttta
     1921 cacggcgcag cacggcgcta attctggcga tcaactcgta caccacaaat ggtttcgtaa
     1981 tatagtcatc agcgccatcc ataatggcac tgactttatc ctccacaccg gagcgtgcgg
     2041 taagaaaaat aaagggaatg gaagccaatt gaggatcctg actcattatc ctacggagct
     2101 caagcccatc cggaggaggc atcatgacat cagaaaggat caggtcgggt ttgatctctt
     2161 ttgcttttgc aaggccgtca ttcccatctt ctgccgaaac aacctgaaaa ccatattttt
     2221 tgatggtggc agatacaccc atcaacaagg cggggtcatc atcaatgatc aagataagtg
     2281 gtttggaatt catgttcatt ttctcgaatg cagtttttgt gtcattccca tagctttacc
     2341 agccagtttg gcgttattgt tcacgaacat gacctgtatg caggaaaacg aaatcaaatc
     2401 actggagatc aacacgcaac gatcaatacg tttgctattt acgtaggtac ctgccgtgga
     2461 attattatcg tacagaatga acttcccatc ttcatagcgt atctcagcat ggaagcgcga
     2521 gacggactct tcctgcaaaa cgacatcatt tcccaattga cgcccaaggc gggtcacctt
     2581 tttctcaagc ggaatcattt gacgattgat gataagaaaa acattctgtt cccgtgaagg
     2641 gataaggttg ttatcttctt cctgtgtcat ggggagatcc tccgtaaaaa ataaatcata
     2701 attcagataa tacaactctc ttatacggtg agtatacata cgaagccgtg aaagcccact
     2761 cagtttagta cttaattcga atacgtaaaa ctacttgata tgcgatttac agcaaaatca
     2821 ccctcaatga ctcatgcaaa caaatgcaca ccaaaaatta agtaaaaata cggatggcgg
     2881 ccttgtgaca gtgccgataa gatgtatttg gtaaaaaacg attgtaataa gcaactgcaa
     2941 tcgactcgtg ttcctgatta accgtacaac tccaaggagt tttcaatatg ttcacgattg
     3001 atacccaatt atcgaacggc ggcgctaaaa cacacttgat cctcgttgcc gacgataaca
     3061 cgatggtttt gcaaatgttg accgattttc tggaaatgca gggatatcag gtatttcaag
     3121 cccgcagcgg agttgaatta cttgaaaaga tctcagagac ccatccagat cttgtgctgg
     3181 tggatattca aatgccgcgc atggatgggc tggaaaccat tcgccgtgtc cgtgcaagtc
     3241 aagacgggca aatctcgtcc atcccgttga tcgccgttac agccctggcc atgcccggag
     3301 accgtgagcg ctgcatgctg gcaggagcca ccgaatatgt gagcaagcct ttaaagttaa
     3361 acgagctttc
//
LOCUS       DAOQJC010000231         3367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_18225_length_3367_cov_11.566123, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000231 DAOQJC010000000
VERSION     DAOQJC010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18225_length_3367_cov_11.566123"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..1367
                     /locus_tag="PLE39_19130"
     CDS             303..1367
                     /locus_tag="PLE39_19130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE70505.1"
                     /translation="MTTYIIRRLLLVPVLLFGVTILIFAMLQFLSPVERTALYVRDFP
                     KNEKAIEGLIKRYGFDQPFYVQYWTWLVGKVDSTTGEREGGILFGNFGYSRSASQPVA
                     ELIQRRFPNTLDLTIWAVAPMILIGIWLGVQAAVHHNGFIDQAARAFSIVGTSFPSFV
                     FGLLMLLIFYANLHWFSPGRLSDEFNRVVESEAFRQYTTVLTIDALLNGRFDVFLDAL
                     RHMFLPILTLSYINWATFLRVTRGSMLETLRMEYVNTARAMGLPERDVIFKHAQPNAM
                     LPVLTLAGFSVVGLLGGVVITETVYNFPGIGSAAARAATSLDVVAMLGFALFNGFILI
                     VANLVVDVLYAFVDPRVRLS"
     gene            1381..2421
                     /locus_tag="PLE39_19135"
     CDS             1381..2421
                     /locus_tag="PLE39_19135"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE70506.1"
                     /translation="MTAATPSGDNTLLGENVATRKIGRVERFLGPENYRIITGLLKTP
                     ASIAGFFLIGFFIFVAIAAPVIMPPVTPNDPYKIPRDGFRSDPRPMGSAWTRNVPDIP
                     FWYTALTGKDKWVHIMGTSEGQYDIFYGMVWGTRTAFQTGLVVTFSTFLIGIIYGSVS
                     AYYGGVVDNIMMRIVDVLLILPGILAVLILAAVLTPKIGKSLIPAMAALIAFGWMGYS
                     RIIRGDILSVKERDYVLAARVIGVKDSRILFRHILPNAIFPTLVLASLAIGDVVLSFA
                     ALSFLGIGTEVGYADWGQVISFARNWITSLNTYWYIIVWPGVTLVLFVMGWNLVGDAL
                     RDVLDPRMRGKT"
     gene            2425..3123
                     /locus_tag="PLE39_19140"
     CDS             2425..3123
                     /locus_tag="PLE39_19140"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNE70507.1"
                     /translation="MQYRFFSQLLLVTIGMALLFAGLFFDIDTVASQAVATPTIDRLA
                     QPTLPADPTQADQGSQVYWLSCLPCHGDRGQGLTDEFRQTYPVEDQNCWISGCHGKRP
                     YDNGFTLPTAIPAIVGPNTLQKFETAASLRSYIFAAMPYWKPASLTEEETWQVTAYIL
                     RESKRLPADMELNASNAASVLVGPPVATSTPQPGPDSVINLDEIQRPVLIVAVLVVAL
                     LLALRIFKTTVNKE"
BASE COUNT          726 a          850 c          815 g          976 t
ORIGIN      
        1 tctacaagga agcgaaccag ctttactacg atctggctgt tggtattccg ttggatgtta
       61 caacctccca cggtttctca cagcgctggg tgaagggtgt cgttcttaac ccgatcttcc
      121 ccggtaacta cttctacacc atgtacaaag actagtcttc ggactggttg atgcaaaaaa
      181 ttcggaggcc tggcaacagg cctccgaacc aatagtgtca ttctgttttg cagaataaaa
      241 agaatggaat attcttttta tttcggaagc cagaatatac tgaagatgaa agaggttgtt
      301 taatgactac atacatcatt cgacgtttgt tgttggtgcc ggtactcctt tttggggtga
      361 cgatattgat ctttgccatg ttgcagttcc tttccccggt agagcggacg gcactttatg
      421 ttcgcgattt cccaaagaat gaaaaagcca tcgaaggttt gatcaaacgc tatggatttg
      481 atcagccgtt ctatgtgcaa tattggacct ggctggtggg caaagtagat agcaccacag
      541 gcgaacgtga aggcggcatc ctttttggaa actttggata ttcccgctcc gcttcacaac
      601 cggttgctga attaattcag cgtcgtttcc ccaacacact tgatttgacc atctgggcgg
      661 ttgctccaat gatcttgata ggtatttggc tgggggtgca ggcggcagtg catcacaatg
      721 gtttcatcga tcaggctgcc cgcgcattta gtatcgttgg aacgtccttt ccatctttcg
      781 tcttcggctt gttgatgttg ctgatctttt atgcgaatct tcactggttc tccccgggtc
      841 gtctttcaga tgaattcaac cgtgtggtcg aatcagaggc cttccgtcag tacaccactg
      901 ttcttaccat tgatgccctt ctaaacggcc gtttcgatgt gttccttgat gcattgaggc
      961 acatgttcct gcccatcctc actctctctt atattaactg ggccaccttc cttcgtgtaa
     1021 ctcgcggttc catgctggaa acactgcgca tggaatatgt caacacagcc cgcgccatgg
     1081 gtttgcctga aagagatgtg atattcaaac atgcccaacc aaacgccatg ttgccggtgc
     1141 tgactctggc ggggtttagt gtggttggtt tgcttggcgg tgtggttatc acagaaacgg
     1201 tttataactt ccctgggatt ggttcggcag cggctcgtgc agccaccagt ctggacgtgg
     1261 ttgccatgtt gggctttgcc cttttcaatg gtttcattct gatcgtggca aacctggttg
     1321 tggatgtgct ctacgcgttc gttgatcccc gcgtacgctt atcataaagg agaattaaaa
     1381 atgacagcag caactcccag cggcgataac accctgttag gcgagaatgt cgccaccaga
     1441 aaaattggac gcgttgaacg ctttttaggc cccgaaaact accgcattat caccggcctg
     1501 ctcaagacac ctgcatccat tgctgggttc tttttgatcg gcttctttat cttcgttgcc
     1561 attgcagcac cggtcatcat gcccccagtc actccaaacg atccatacaa aattccgcgt
     1621 gatggcttcc gttcagatcc ccgccccatg ggctcggcct ggacccgcaa tgtcccggat
     1681 attccatttt ggtacacggc attgaccggt aaagataaat gggtccacat tatgggcacc
     1741 tctgaaggcc aatacgatat cttctacggc atggtttggg gtacccgcac agcctttcag
     1801 accggtttgg ttgtaacctt ctccaccttc ctcattggca ttatctacgg ttccgtttct
     1861 gcttattatg gcggagttgt ggataatatt atgatgcgca ttgtggatgt gttgttgatc
     1921 ctgcccggta ttttggctgt gctcattctt gctgcggtgc tcacaccaaa gatcggtaag
     1981 agtttgatac cagccatggc ggcgctcatc gcgtttgggt ggatgggata ttcccgcatc
     2041 attcgcggcg atatcctgtc tgtgaaggag cgcgattatg ttctggcggc ccgagtgatc
     2101 ggcgtcaagg atagccgcat tcttttccgc cacattcttc ccaacgccat cttccccacc
     2161 cttgtgttgg cttcactggc cattggtgat gtggtgctct cctttgcagc cctttccttc
     2221 ctcggcattg gtacagaagt tggttacgct gactggggtc aggtaatttc cttcgcccgc
     2281 aactggatca ccagtttgaa tacctactgg tatatcattg tctggccagg ggtgaccctc
     2341 gtattgttcg tcatgggttg gaatctggtt ggcgatgccc tgcgcgatgt gctggatccc
     2401 cgcatgcgcg gcaagactta atctgtgcag tacagattct tttctcaatt attgttggtc
     2461 actattggaa tggccctgct ctttgcaggg ctattcttcg atattgacac agttgcttcc
     2521 caggcagttg ccacacccac catagatcgg cttgcccaac ccaccctgcc tgccgacccc
     2581 acacaggccg atcaaggttc gcaggtgtat tggctatcct gcctgccttg tcacggtgat
     2641 cgcggtcagg ggttgacgga tgaattccgc caaacttatc cggtggaaga tcagaattgc
     2701 tggatttcag gttgtcatgg caaacgaccc tacgataacg gatttacact gccgactgcc
     2761 attcctgcca tcgtaggtcc caacactttg caaaaatttg agacggcggc cagtttaaga
     2821 tcttatatct ttgcggccat gccttattgg aaacctgcca gcctgaccga agaagagaca
     2881 tggcaggtca cggcatacat acttcgtgaa agtaaacgcc tgcctgcgga tatggaactc
     2941 aacgcctcca acgccgcctc cgtgttggtc ggccctcctg ttgcaacttc cacccctcaa
     3001 cctggtcctg actctgtaat caatctagat gagatacaga gaccagttct aatagttgct
     3061 gttcttgttg tagcgctgct tcttgctctg cgcattttca aaaccactgt caataaagaa
     3121 taatgaatta agccgcccgt aaaggcggct taatcgtata attgaaacgg acttgatttg
     3181 ggcaggtacg atcttttttg atacctgctt gtctgtaagt aaaaaggagg tggttgtttg
     3241 cagatattgg tatttttgaa tgttttatgt ccgccaatgt agttcgtaaa acaaaatctc
     3301 gtgaggagaa caaaaaatgc gtaaaatgtt tgctttactg agccttctcg tcatcgccag
     3361 catggcc
//
LOCUS       DAOQJC010000232         3188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_19933_length_3188_cov_8.782317, whole genome shotgun sequence.
ACCESSION   DAOQJC010000232 DAOQJC010000000
VERSION     DAOQJC010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19933_length_3188_cov_8.782317"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..860
                     /locus_tag="PLE39_19145"
     CDS             183..860
                     /locus_tag="PLE39_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70508.1"
                     /translation="MSKLLSRFSYSNLKEQLMKIWPVVLVGIFVSLGGAGITVLVAAL
                     TDNPIWKLAKDPAEVMQFPSYIGMLSNWGVLLWIASAVICLFGVAVLKQYEVPKDTLR
                     FITGSGLLSLILGIDDLYRLHDKIFPKMFHLPEKFFYILYIFIFLLYLAMFAARIMEY
                     EFLLFGAAFFLLVVSRRIVVIIPFFNQFMTTGDMLKYFGIVFWLAFFYRTVLHEITSL
                     INKNKTA"
     gene            1202..2473
                     /gene="serS"
                     /locus_tag="PLE39_19150"
     CDS             1202..2473
                     /gene="serS"
                     /locus_tag="PLE39_19150"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNE70509.1"
                     /translation="MLDINLIRETPDVVRKALKDRQMDASPVDAILQLDEKRRTLLGQ
                     VETLKAERNTVSKEIGQMKDAAARQSKIEAMRVVGDQIAELDKQVAEVETQLSDLTSA
                     LPNIPDARTPYGKDDSENVILRTVGEPRKFDFKPLPHWDLGPALGILDFERGTKLTGS
                     RFYVLQKAGARLQRALIAWMLDLHTRQGYQEEYLPFMVKTAVVFGAGQLPKFADNLYK
                     DHEEDYYFVPTAEVPLTGLHMDEIVEEATLPKLYTAYTPCFRREKMSAGRDVRGIKRG
                     HQFDKVEMYTYCKPEESEAQHEKMLKDAEETCALLGVPYRVKRLCTGDLGFGSTMTYD
                     LELWAPGCDEWLEVSSISNVTDFQARRANIKYRPSDGGKAKFVHTLNGSGLGLPRTLI
                     AVMENYQQADGSVVVPEVLRPWMGGIDVIKP"
     gene            2539..3060
                     /locus_tag="PLE39_19155"
     CDS             2539..3060
                     /locus_tag="PLE39_19155"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HNE70510.1"
                     /translation="MDPEALKLFAEVFLEVTTFVILLFGLFGLLIPVFPGLTVMWIAT
                     LVYAIVRLSVGNMTWVGWLLFVVITLLMVGGNIADNIIIAKHVRDKNVPWSSIIWAFA
                     AGIVVSLFLTPLAGIIASPIGLYLAEWYRLKEKQPAFDNTKAWMTGWGWSLAARFGIG
                     VVMIAFWGLWAWI"
BASE COUNT          756 a          748 c          850 g          834 t
ORIGIN      
        1 aaaagtctga aagattaacc acggagacac agagaaaaaa ataaaaactc cgtccgcagg
       61 atgctttgca ctgcctcagt gtctttgtgg ttcaaaatgg cttttcaaag atattccata
      121 caacttatgc aagtggttaa atataaaaat tattgtacaa attgatttag gataggtata
      181 ttgtgtcaaa attgctttcc cgtttttcgt actcaaacct caaagaacaa ctaatgaaga
      241 tctggcctgt ggtccttgtt gggatttttg tttctttggg cggggctggt atcaccgtat
      301 tggtcgctgc attgaccgat aaccccattt ggaaactggc taaagaccca gccgaagtaa
      361 tgcagtttcc ctcctatatc ggtatgcttt ccaattgggg cgtccttttg tggattgctt
      421 cagcagtgat ttgccttttt ggtgtggccg ttttgaaaca atacgaagtg cccaaagata
      481 ctttgaggtt tataacaggt tcggggctgt taagcctgat cctgggaatt gacgatctct
      541 accgtctgca cgacaaaata tttcccaaaa tgttccacct gcctgaaaag ttcttctaca
      601 tactttatat ctttattttt ctcctgtatc tggccatgtt tgccgcccgt atcatggaat
      661 atgaattttt gttgtttggc gccgcctttt ttttgctggt ggtttcccgc cgtattgtgg
      721 tgatcattcc gttcttcaac cagttcatga ccacgggtga catgttgaaa tacttcggca
      781 ttgttttctg gctggcattc ttttatcgaa cggtactgca tgagatcacc agcctgataa
      841 acaaaaacaa gactgcttga cagcccattg accgcgcgta taattcagcc caagtagacc
      901 cacgcgaaga caagtatctg cctccccttt cagagagcca tcggctggtg cgagatggca
      961 ggccaagcag tgaaatcccc ttcgttatgt cgctcttgca gggatacgca ataattgcta
     1021 tcgtaagaga gtcgggtgcg cccgttatcg cgcaaatcaa gacctccacc caatcttccc
     1081 taaccattta gggaaggaga tgaaatcggg aaggggtaaa agcaggtggc accacgagac
     1141 gccccctcgt cctgcaaatg gattgggggc gtattctgtt aaatgcacat ggaggtgcaa
     1201 catgttggat atcaatttaa ttcgcgaaac accggatgtt gtccgcaagg cgttgaaaga
     1261 ccgccagatg gacgcctctc cggtggatgc cattcttcag ttggacgaaa aacgccgcac
     1321 cctgcttggt caggtggaaa cgctcaaggc agagcggaac actgtctcaa aggagatcgg
     1381 gcaaatgaaa gacgcagcag cccgacagtc gaagatcgag gcgatgcggg tggtcggaga
     1441 tcagatcgca gagttggaca agcaggtcgc tgaagtcgag actcaattga gcgacctcac
     1501 atccgcgctc ccaaacatcc ccgatgctcg tacaccctac ggcaaagatg acagtgaaaa
     1561 tgtgatcttg aggacggtgg gggagccgcg taaatttgat ttcaaaccgc ttccacactg
     1621 ggaccttggg cctgctcttg gaatccttga ttttgagcgc gggacaaagt tgaccggttc
     1681 ccgtttttac gtgttgcaaa aagcaggcgc ccggttacag cgcgccctga tcgcatggat
     1741 gcttgacctg cacacccgcc agggctatca ggaagagtac ctgcctttca tggtgaagac
     1801 cgcagttgtg ttcggtgcgg ggcagctgcc aaaatttgca gataatctgt acaaggatca
     1861 tgaagaagat tactactttg tgccgaccgc tgaagtcccc ctgaccggcc tgcacatgga
     1921 tgagatcgtc gaagaagcca cgctgccgaa attgtacaca gcctacactc catgcttccg
     1981 ccgtgaaaag atgagcgcag gccgtgatgt gcgcggaatc aaacgcgggc atcaatttga
     2041 caaggttgag atgtacacct attgcaagcc ggaagaatcg gaagcccagc atgaaaagat
     2101 gctgaaagat gccgaagaaa cctgcgcttt gttgggtgtt ccgtatcgcg tgaagcgcct
     2161 ttgcaccggt gacctcgggt ttggttcaac catgacctat gaccttgaac tgtgggcgcc
     2221 aggctgcgat gaatggcttg aggtttcttc gatctcgaat gtgaccgatt ttcaggcaag
     2281 gcgcgccaat atcaagtacc gccccagcga cggaggcaag gcgaaattcg tacatacatt
     2341 gaacggatcg ggtttgggtc tgccgcgcac gttgatcgcc gtcatggaaa attatcaaca
     2401 agccgatgga tctgtggtgg tgcccgaggt tctgcgcccg tggatgggcg gcattgatgt
     2461 gatcaagccg taaatagaat cgacctgctc ctctcccaat atccgcgggg gaggggcagg
     2521 gaacatggag gaagccatat ggaccctgaa gctctaaaac tctttgcaga agtatttctg
     2581 gaagtgacca cgttcgttat tttgcttttc ggtttgtttg gcttgctcat tcccgttttc
     2641 cccgggttga cagtgatgtg gatcgccaca ctggtctatg ctattgtgcg gctttccgtg
     2701 ggaaatatga catgggtggg ttggctgctt ttcgtggtca tcaccttgtt gatggtcggc
     2761 ggtaatatcg cggataacat catcatcgcc aagcacgtgc gcgataaaaa cgtaccctgg
     2821 agctcgatca tttgggcatt tgcggcgggg atcgtggtca gtttgttcct caccccgctg
     2881 gctggaatta ttgcctcccc gatcgggttg tatttggcgg aatggtatcg cctcaaggaa
     2941 aagcaacccg cctttgataa caccaaagca tggatgacgg gttgggggtg gtcactggcg
     3001 gcaaggtttg gaattggtgt ggtgatgatc gccttttggg gcttgtgggc gtggatatga
     3061 gtagcgcgga cttcagtccg cttttgccgc ggattctgct gaactttgcg gaatccgcgg
     3121 caattctatt ttaatggtca gtcatcttta tcttttaatt ttttctgcgc atcgcgtatg
     3181 gcgagaaa
//
LOCUS       DAOQJC010000233         3120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_20702_length_3120_cov_9.597716, whole genome shotgun sequence.
ACCESSION   DAOQJC010000233 DAOQJC010000000
VERSION     DAOQJC010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20702_length_3120_cov_9.597716"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PLE39_19160"
     CDS             <1..344
                     /locus_tag="PLE39_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70511.1"
                     /translation="EDAPMNCGGFQGGGNPNAPNQFFFGGGGGGGGGGGGGFRRGIDE
                     ETLKQVADMTGGEYFAASSTGELQNVFNNLPVQLMTVTETTEVSVFFAAIGAVLILLA
                     LILSMLWHPIA"
     gene            complement(522..1988)
                     /locus_tag="PLE39_19165"
     CDS             complement(522..1988)
                     /locus_tag="PLE39_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNE70512.1"
                     /translation="MTTIDLTVHKDRRARAIQRAKERNIIIPTYAQMKDPSKIPAKIK
                     EELTKIGLWDIHPRNLFRINWHNQPTASGGTFGGVNYLELPSSLTGVKARIIAVVGKW
                     FPTGAHKVGAAFSCLVPRLVTGQFDPTTQKAVWPSTGNYCRGGAYDSALLGCESIAIL
                     PEGMSKERFEWLAKVAGETIKTPGSESNVKEIFDKCKELATSGQDLMIFNQFEEFGNY
                     LWHYEVTGHAMEEVFKAVAGKHGRFRGIASATGSAGTIASGDYMKQLFPESKIVASEA
                     LQCPTLLENGFGAHRIEGIGDKHVPWVHNTKNTDIVTAIDDNAVVNISRLFNEENGRA
                     YLAEKGVPESVISNLDLLGFSGISNVLSCIKTAKYYEMDENDVMITMLTDSMELYRSR
                     LHEMHQEFGEYSEKDAAADFARYLHGQSTDNMLELTYPERRRVHNLKYYTWVEQQGKT
                     YEEIQNQWYQPDYWTSVQKQANEIDELIVEFNKEVGLV"
     gene            2143..2526
                     /locus_tag="PLE39_19170"
     CDS             2143..2526
                     /locus_tag="PLE39_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70513.1"
                     /translation="MKAMKFWWLIFVFPVVIALMVTSIALVPNNPLRKVAGMLVCPSG
                     SILKVTASDTTWYEDGFIREGTGYSFDCVNLSGAPVENNIDTVFTVLLLATILSPFVI
                     VLARVFAPQKQVKHESMYDGKMKVG"
     gene            2528..2989
                     /locus_tag="PLE39_19175"
     CDS             2528..2989
                     /locus_tag="PLE39_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70514.1"
                     /translation="MNNTKTTAISGCVIWFLLISVIGSCVMPVFFMVGSFTSFSEYAI
                     KTTGGFLCPAGTTPKSYSYSSTSFDEFGNPENGTAYELHCVDASGTVVKSDPIFYAFL
                     WIGIWALIGAVVSGLLTFVFAVPGGMLVTKLLNKLKRPSTNITSSDYPKFD"
BASE COUNT          713 a          684 c          832 g          891 t
ORIGIN      
        1 aggaagatgc ccccatgaat tgcggcggct ttcaaggtgg aggcaacccg aatgccccca
       61 atcagttctt cttcgggggc ggtggtggag gcggaggtgg gggtggcggt ggtttccgca
      121 ggggaattga cgaagagacc ctgaaacaag tagccgatat gacaggcggg gaatattttg
      181 cggcttccag caccggagag cttcagaatg ttttcaataa cctgcccgtg caattgatga
      241 ccgtcactga aacgacggag gtcagtgtgt ttttcgctgc gatcggtgcc gtgcttatat
      301 tattggctct catcctttcg atgctttggc atccgattgc ataaatagaa ctcttgcata
      361 agtccatttt tttaccacga aggcacaaag tctcgaagac tcaaagaaaa aaatcttagt
      421 gacttagtgc cttggtgact ttgtggtcta taacacttta cttatacaag aggtcaaata
      481 aaaaaatcct ccgagcctga aaactcggag gattttttgt cttacaccaa tccaacttcc
      541 ttgttgaact caacgatcaa ttcgtcaatt tcattggcct gcttttggac gctggtccag
      601 taatcaggct gataccattg gttttgaatc tcttcatagg ttttgccctg ctgttcaacc
      661 catgtgtagt atttcaagtt gtgaaccctg cggcgttcgg ggtaggtaag ctcaagcatg
      721 ttgtcagtgg attgtccatg caggtagcgg gcgaaatcgg cggcggcgtc tttttcgctg
      781 tattcgccga attcctggtg catttcatgc aggcgtgagc gatagagttc catcgagtct
      841 gtcaacatgg tgatcatgac gtcgttctcg tccatttcgt aatacttggc ggtcttgatg
      901 caggaaagga cgtttgaaat gccagagaaa ccgagcaggt caaggttgga gatgacggat
      961 tcgggcacac ccttttcagc gaggtaggcg cgtccgtttt cttcattgaa cagacgggag
     1021 atgttgacta cggcgttatc gtcaatggcg gtgacaatgt ctgtgttctt ggtgttgtga
     1081 acccagggca cgtgcttatc gccgatgcct tcgatgcgat gcgcgccgaa gccgttttcc
     1141 aacagggtgg ggcattgcag ggcttcgctc gccacgatct tgctttcagg gaagagttgt
     1201 ttcatgtagt cgccgctggc gatggtgcca gccgaacctg ttgcagaggc gatgccgcgg
     1261 aaacgtccat gcttgcctgc aaccgctttg aagacttctt ccatggcgtg gcctgtcact
     1321 tcatagtgcc agagataatt gccaaattct tcaaattgat tgaagatcat caagtcctga
     1381 cctgaggtag ccagttcctt gcatttatca aaaatttctt ttacgttgga ttcagagcca
     1441 ggggttttga tggtttcgcc tgcaactttg gccagccatt caaaacgctc ttttgacatg
     1501 ccttcgggca aaatggcgat cgattcgcag ccaagcaggg cggaatcata tgcaccgccg
     1561 cgacagtagt tgcctgtgga aggccacacc gctttttggg tcgtcgggtc aaactgacct
     1621 gtgaccagtc tcggcacaag gcaggaaaaa gccgccccga ctttgtgtgc gccggtgggg
     1681 aaccatttgc cgacaacggc aatgatacgg gctttgactc ctgtcagcga agagggaagc
     1741 tcaagatagt tgaccccgcc aaaggttccg cctgaagcgg tgggttggtt atgccagttg
     1801 atgcggaaaa ggttgcgggg gtgaatatcc catagcccga tcttggtcaa ttcctccttg
     1861 attttcgcag gaattttgga agggtctttc atttgggcat aggtggggat gatgatgttg
     1921 cgctcttttg cgcgttgaat ggcgcgggcg cggcggtctt tatgaacggt caggtcaatc
     1981 gtggtcatgg ggttctcctg aagtagttgt cagacaactt tgattatagc gcacaagtga
     2041 gttcaataca actatctgca cacttggtac aaacaggtga ttttgtttgg gtttcatcag
     2101 tctataatct ggcttgcaaa aataatttat tgaggtgaat taatgaaggc tatgaagttt
     2161 tggtggttaa tctttgtgtt tcctgttgtg attgccttga tggttacatc cattgcgctt
     2221 gttcccaaca acccgctgcg gaaagttgca gggatgttgg tatgcccatc tggttccata
     2281 ctcaaggtga ccgcgagcga cacaacctgg tatgaagatg gtttcatccg ggaaggcaca
     2341 ggatattctt tcgattgcgt gaatctctcg ggtgctcccg tggaaaataa tatcgatacg
     2401 gttttcactg tgttgttgct tgcaacgatc ctttcaccgt ttgtgattgt gcttgcccgg
     2461 gtgtttgctc cgcagaaaca ggtaaagcac gaatcaatgt atgatggcaa aatgaaagta
     2521 ggttgatatg aacaacacca aaacgaccgc catttccggg tgtgtgatct ggtttttact
     2581 gatttccgtc atcggcagtt gtgtgatgcc ggtgttcttt atggtgggga gttttacatc
     2641 gtttagtgaa tatgccatta agacaacggg tggttttctg tgccctgctg gcacaacacc
     2701 gaagagttat tcctactcaa gcacttcttt cgatgagttt ggcaatccag aaaatggcac
     2761 tgcatacgaa ctgcattgtg tggatgcgag cggaactgtt gtgaaaagcg acccgatctt
     2821 ctatgccttt ctttggatcg gcatctgggc attgatcggt gcggttgttt caggtttgtt
     2881 gacctttgtc tttgccgtac ccggcggcat gctggtgaca aaattactga ataaactgaa
     2941 acgcccttcc accaatatca catccagcga ttacccgaag tttgattgaa ccattaaaca
     3001 tgacttaccc cgaaattaag gtttcgcggg taagttcgta ttttaagaac aagtaggacg
     3061 cagaaaacgc tgattgttgc ttttgatcag cgttttctgc atcctcaatt taatttaagt
//
LOCUS       DAOQJC010000234         3107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_20848_length_3107_cov_7.560616, whole genome shotgun sequence.
ACCESSION   DAOQJC010000234 DAOQJC010000000
VERSION     DAOQJC010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20848_length_3107_cov_7.560616"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PLE39_19180"
     CDS             complement(<1..783)
                     /locus_tag="PLE39_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70515.1"
                     /translation="MKDKNSLNAEERALAEMLAESAAQVEIGASFQNELEKKLMNAHK
                     PKTGFGLFSFGRAASTLASVLGLAILSLIFIWAIRAISSKPQPAIGNPFAIECPVTTP
                     NGSTPPGETATYEGYLGNGKLWTSLWPEGKIIMTPENRNADGSLSMKWLWWRGVSGPL
                     SIEGHRLDAEAEPLRSFISSGYGDIDFQVSELIFPTTGCWEVTGYAGNASLTFVTEVI
                     FEAATPTLIPTIVTQSTPISPTATFEVLPTAAATLFRDDFEGT"
     gene            complement(780..1364)
                     /locus_tag="PLE39_19185"
     CDS             complement(780..1364)
                     /locus_tag="PLE39_19185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNE70516.1"
                     /translation="MTTTMTTHPHSPPADEVDLARLARTNAEAFGELYRRHVTRVYRY
                     HMAHTGNIKDAEDLTSQTFIAALENIGSFRGTGSFAAWILGIAARKKAMFFRRIGTRP
                     EVQMEAADQIPNPGLPTEQLANQRLRLDSITRALKQISPERADAITLCFFSELTYAEV
                     GIALNKSEAAAKMLVIRGLQDLRERTSLAFEVEP"
     gene            complement(1419..2009)
                     /locus_tag="PLE39_19190"
     CDS             complement(1419..2009)
                     /locus_tag="PLE39_19190"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNE70517.1"
                     /translation="MIILTYLAVGASAISGALEARKHEMDIVGATTIAFVTAFGGGTI
                     RDLLLGRTPVFWVIDPWLTVVTFLVGAIAFYRLDRISNRLLIIADAIGLGLFSILGAT
                     YTLQLELSPIVAVLMGVVTGMFGGVLRDLLCNRIPNVFRQSTELYATCSFIGTSVFIL
                     LRSFTVGSFTASVIGAAIIFTMRLAAVRFKVTLPAP"
     gene            complement(2027..2503)
                     /locus_tag="PLE39_19195"
     CDS             complement(2027..2503)
                     /locus_tag="PLE39_19195"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNE70518.1"
                     /translation="MSKIKQPNSRHCFICGLENPVGLHLHMYEVEPGVIETQYIAPDH
                     FQGYPGVLHGGIVAALIDEISGRAQMGSDPANPRFMFTGKLEVKYRKNVPINKLLKIV
                     GKAGKSKRNIAEAWAGIYDAETNELLAEGSTVSVDVPKDQFEVSKLEELGWKVYPD"
     gene            2526..>3107
                     /locus_tag="PLE39_19200"
     CDS             2526..>3107
                     /locus_tag="PLE39_19200"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE70519.1"
                     /translation="MNQPKIFITRVIPSPGLNLVREHFLPVAWADEMPPTREQLLEHV
                     RGVDGLLCLLTEKIDGELMDAAGPQLKVISSMSVGVDHIDVAAATARGIPVGNTPGVL
                     TDATADQAFALLLAAARRVTEAERFLRAGKWVTWQPSLLLGADLVGATLGIVGFGRIG
                     QAIAKRAQGFDLRVLYHSPNAQPAYGAKPVDLDT"
BASE COUNT          682 a          788 c          885 g          752 t
ORIGIN      
        1 tgtaccttcg aaatcatccc tgaataatgt ggctgcggcg gtgggcaaaa cttcaaatgt
       61 cgccgtgggt gagatggggg tactttgtgt cacgatggtt gggattagtg taggcgttgc
      121 cgcttcgaag atgacttcgg taacgaatgt aagcgaggcg ttgcctgcgt aaccagtgac
      181 ttcccaacag cctgtggtgg ggaagatcag ttctgagacc tggaaatcaa tatctccata
      241 ccccgacgag atgaatgagc gtaagggttc ggcttctgca tccagtcggt ggccttcgat
      301 gcttaacggt ccactgacgc cgcgccacca gagccatttc atgctgagcg acccatcggc
      361 gttgcgattc tcgggggtca tgataatttt tccttcaggc cagaggctgg tccacaattt
      421 accattcccg aggtaccctt cataggttgc agtttcgcct ggtggggtgc tgccatttgg
      481 tgtggtgaca ggacactcaa tggcaaacgg atttccgatg gcgggttgag gtttggatga
      541 aattgcgcgg atggcccaga taaatatcaa agaaagtatg gcaagcccca gcaccgaagc
      601 cagcgtggag gcggctctgc cgaatgaaaa taaaccaaac cctgttttgg gtttatgggc
      661 gttcatgagt tttttctcca gttcgttttg aaacgaagcg ccgatttcca cctgtgcggc
      721 gctttctgcg agcatttcag ccaacgcgcg ttcttcagcg ttcagcgagt ttttgtcttt
      781 catggttcca cctcaaaggc gagcgaggtc cgttcgcgca ggtcttgcag cccgcggatg
      841 acgagcattt ttgcggcggc ttcactttta ttgagcgcaa tgccaacctc ggcgtaagtg
      901 agctcactga aaaaacaaag ggtgatggcg tctgcccgct cgggactgat ctgtttcagg
      961 gcgcgggtga tggaatcaag cctgaggcgc tgattcgcca attgctcggt tggcaggcct
     1021 gggtttggaa tttgatccgc cgcttccatc tggacctctg gtctggttcc gattcttcgg
     1081 aagaacatcg ccttctttcg ggctgcaatc cccaaaatcc atgccgcaaa ggagccagtt
     1141 ccgcgaaatg agccaatgtt ttcgagtgca gcgatgaagg tctgtgaggt gaggtcttcc
     1201 gcatctttga tgttgcccgt gtgcgccatg tgatagcgat agacgcgtgt tacatggcgg
     1261 cgatacagtt cgccaaaggc ttctgcattt gtgcgggcca aacgggcaag gtcaacttca
     1321 tcagcaggcg ggctgtgtgg atgagttgtc atggtcgtgg tcatggacct cctgttacgt
     1381 gtatgttgca ggaggtatgg aaaaagtaac agggaatttt atggtgcggg gagtgtcact
     1441 ttgaagcgga cggctgcgag gcgcatggta aaaatgatcg ccgcgccgat cacagatgcc
     1501 gtaaatgatc caacggtgaa tgacctgagc aaaataaaaa cactggtgcc gatgaaggag
     1561 caggtggcat acaactctgt gctttgacgg aacacatttg ggatgcggtt gcaaagcagg
     1621 tcgcgcagca caccgccgaa catgccagtg accacgccca tcaacactgc cacaatgggt
     1681 gaaagctcaa gctgcagggt gtacgttgcc ccgagaatgc tgaacaaacc aagcccgatc
     1741 gcatcagcga tgatgagcag tctgtttgaa attctatcga ggcggtaaaa cgcaattgcc
     1801 cccactaaaa acgtgaccac ggtcagccat gggtcaatga cccaaaaaac gggtgtgcga
     1861 cccagcagca ggtcgcgaat cgtcccgccg ccgaaggctg tgacaaacgc aatggtggtg
     1921 gccccgacga tgtccatttc atgtttgcgc gcttcgagcg cacccgaaat ggccgaagcg
     1981 cccacagcca gatatgtgag gatgatcaaa atattttcca cgaagattaa tcggggtaaa
     2041 ccttccagcc caattcttcg agttttgaaa cttcgaactg gtccttcggc acatccactg
     2101 aaaccgtcga accttcggcg agcagttcat tcgtttcggc gtcgtaaatc cccgcccatg
     2161 cttcggcgat gtttcgtttg cttttgcccg ctttgccgac gatcttcaat agcttgttga
     2221 tcggcacatt cttgcgatac ttcacttcaa gctttcccgt aaacatgaag cgcggattgg
     2281 cagggtcgct gcccatttgc gcgcgtcctg agatctcatc gatcaatgcg gccacaatgc
     2341 cgccatgcag cacgcccggg taaccttgaa aatgatcggg cgcgatgtac tgcgtttcaa
     2401 taacgcctgg ttccacttcg tacatgtgca aatgcaaacc aactggattt tccaatccgc
     2461 aaatgaagca gtgacgtgaa tttggttgtt ttatttttga catggtacgg attatactcg
     2521 gaggcatgaa tcagccgaaa atttttatca cccgcgtcat tcccagccct ggcttgaatc
     2581 tggtcaggga gcattttctc cccgtggcat gggctgacga aatgccgccc acccgtgaac
     2641 aactccttga gcatgtgcgc ggcgtggatg gtctgctttg cctgctcaca gaaaaaatag
     2701 acggtgaact catggacgcg gctggtccgc agttgaaggt tatcagcagc atgtcggtcg
     2761 gcgtggatca tattgatgtg gctgctgcca cggcgcgtgg aattcctgtg ggcaatacgc
     2821 ccggtgtgct caccgatgcc acggcagatc aggcttttgc tttgttgttg gcggctgccc
     2881 gccgcgtcac cgaggcagag cggtttttgc gggcaggcaa gtgggtcaca tggcagccga
     2941 gtcttttgct tggtgccgat ctggtcggtg ctacacttgg cattgtcggt tttggcagga
     3001 tcggtcaggc gattgccaaa cgggcgcagg gattcgatct gcgggtcctt tatcacagcc
     3061 caaatgcaca gcctgcctat ggcgcaaaac ctgtggatct ggacacc
//
LOCUS       DAOQJC010000235         3083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_21119_length_3083_cov_9.297886, whole genome shotgun sequence.
ACCESSION   DAOQJC010000235 DAOQJC010000000
VERSION     DAOQJC010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21119_length_3083_cov_9.297886"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /gene="hypF"
                     /locus_tag="PLE39_19205"
     CDS             complement(<1..1495)
                     /gene="hypF"
                     /locus_tag="PLE39_19205"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNE70520.1"
                     /translation="MRSIRVHVTGIVQGVGFRPFVYGLAVRLGLRGWVLNTSAGVDIH
                     VEGAEVQVALFIEKLSSELPPLATLDSVTVKEAEVENHSVFTIQPSNHIEGAFQPVSP
                     DVALCADCERELFDPQNRRYLYPFINCTNCGPRFTIIKDLPYDRPATTMADFPLCEDC
                     RAEYENPTDRRFHAQPVACPTCGPFVQLRSLSTGHVSNIELRLSAVLKTRQFLREGRV
                     VAIKGLGGFHLACDAMNERAVNELRQRKGRAGKPFALMFANLDTVMQYCEINEEEAKL
                     LTGHEKPIVLLKLKAHTSLAANVAPHMDTLGAMLPYTPLHHLILNQTDEKLNAQSVPP
                     VLVMTSGNLSEEPISITNEDAFEKLSPLADAFLLHNRDIHVRCDDSVVRADGRGITFL
                     RRARGYAPYPVLLPFESRPTLAVGGELKNTFCLTRDRYAFLSQHIGDMENVEVYESFE
                     QNVERLSHLFRVQPQVIAHDMHPNYFTTQWAQKMEGRRVPVQHHHAHI"
     gene            complement(1512..2168)
                     /gene="hypB"
                     /locus_tag="PLE39_19210"
     CDS             complement(1512..2168)
                     /gene="hypB"
                     /locus_tag="PLE39_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HNE70521.1"
                     /translation="MTTRIPVVEKILSANDRMAERNRNFLDQAGVFAINIMASPGAGK
                     TSTILKTLETLRGRLKLGVVEGDLASSIDAEKAAAAGLPAVQINTGGECHLDAAMLSD
                     ALPTLPLSEIDLLLVENVGNLVCPANFQIGTHLNVLIASIPEGDDKPYKYPGMYRDVQ
                     ALIINKIDLLPYVPFNMEYFRKGVEVLNPGLVTFAVSAKTGEGMEAWTDWLVQNIKSK
                     "
     gene            complement(2188..2568)
                     /gene="hypA"
                     /locus_tag="PLE39_19215"
     CDS             complement(2188..2568)
                     /gene="hypA"
                     /locus_tag="PLE39_19215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNE70522.1"
                     /translation="MKIPFLAIIKVVAMHELAVTESILDISLRHAKEAGASRVTDINL
                     VIGQFASIVDDSVSFYWNIVAEGTMAQGARLHFERIPAEMACSACGHTFHPDDETFEC
                     PSCASMQVRITQGEEFRVDSIDVE"
     gene            2824..>3083
                     /locus_tag="PLE39_19220"
     CDS             2824..>3083
                     /locus_tag="PLE39_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HNE70523.1"
                     /translation="MEIPRHWRLKQQRYGLVGEVCPHCDYKIFPPRDVCPNCGDEAKE
                     LFTFSGKGQVYSYTTVYEAPAGYDANAPYTVAIVKLDEGPMVT"
BASE COUNT          746 a          769 c          815 g          753 t
ORIGIN      
        1 caatgtgcgc atgatggtgt tggacaggca cccgcctgcc ttccattttt tgcgcccact
       61 gagtcgtgaa ataatttgga tgcatatcat gagcaatgac ctgcggctgc acgcggaaca
      121 gatgcgaaag ccgctcaaca ttttgctcaa aagactcgta cacttcgaca ttttccatat
      181 cgccgatgtg ctggctcaga aacgcgtagc ggtcgcgggt gaggcagaag gtattcttca
      241 actcgccgcc cactgccagt gtgggcctgc tctcgaatgg cagcaaaacg gggtatgggg
      301 catatccccg cgcacgtcgc aggaaggtta tgccgcggcc atcggcacgc accaccgaat
      361 catcgcagcg cacgtggata tcgcgattgt gcaacaggaa ggcatcggcc agcggggaga
      421 gtttttcaaa cgcatcttca tttgtgatgg agatgggttc ttcgctaagg ttaccgctgg
      481 tcatcaccag cacaggcggc acagactggg cgtttagttt ttcatctgtc tgattgagaa
      541 tgagatgatg aagcggtgta tatggcagca ttgcgccgag ggtatccatg tgcggagcaa
      601 cattggcagc caggctggta tgtgccttga gcttcaataa aacgatcggt ttttcatgcc
      661 cggtcagtaa tttcgcttcc tcttcattga tttcgcaata ttgcatcaca gtatcgaggt
      721 tggcaaacat cagcgcaaat ggcttgcctg ccctgccctt gcgttgacgc aattcattga
      781 cagcgcgttc gttcatggca tcgcaggcaa gatgaaatcc acccaaccct ttaatggcaa
      841 caaccctgcc ctcccgcaga aattgacgcg ttttcaagac cgccgaaagg cgcaattcaa
      901 tattcgaaac atgccccgta gacaggcttc gcaattggac aaacggtccg caagtggggc
      961 aggccacagg ctgggcatgg aatcttcggt cggttgggtt ttcgtattca gcacggcaat
     1021 cttcgcacag ggggaaatct gccatcgtgg ttgcaggccg gtcatatggc aggtctttga
     1081 taatggtgaa acgcggacca cagttggtgc agttgatgaa cgggtacagg tagcgcctgt
     1141 tttgcgggtc gaagagttcg cgctcacaat cggcacaaag ggcaacatca ggtgaaacgg
     1201 gctggaatgc accttcaata tgatttgaag gttgaatggt aaaaaccgaa tggttttcaa
     1261 cctcagcctc tttaactgtg accgaatcca gcgttgccaa tggaggcagt tcactcgaaa
     1321 gtttttcaat gaataaagcc acctgtacct ccgccccttc cacatgaata tccacacctg
     1381 ccgatgtatt gagcacccag ccacgcaaac caaggcggac agccagcccg tacacaaacg
     1441 gacggaagcc cactccttga acgatgccgg taacgtgaac gcgaatgctg cgcattggag
     1501 aaattagtgg atcatttgct ttttatgttt tgtacaagcc agtcggtcca tgcttccatg
     1561 ccttccccag ttttggcaga caccgcaaac gtcaccagcc ccgggttcag cacttccaca
     1621 cccttgcgga aatattccat attgaaaggt acatagggca gcagatctat tttattgatg
     1681 atgagtgcct gcacatcgcg gtacatgccc gggtacttat acggtttgtc gtcgccttcg
     1741 gggatggaag cgatcaacac gttgagatgg gtgccgatct gaaaattagc cgggcagacg
     1801 agattgccca cattttcgac gaggagcaga tcgatctcac ttaacgggag agttggtaag
     1861 gcatccgata acatggctgc atcgagatga cattctccgc cggtgttgat ctgcacagcg
     1921 ggcaaacctg ccgccgcagc tttttccgcg tcaatggatg aggccaggtc accttccact
     1981 acgccgagtt ttaaccgtcc gcgcagggtt tcgagggttt ttaagatagt gctggttttc
     2041 cccgctccgg gggaggccat gatattgatg gcaaataccc ccgcctggtc aaggaaattg
     2101 cgattacgct ctgccatgcg gtcattggca gagaggatct tttctacaac tgggatgcgt
     2161 gtggtcattt ttctccgtgg tatgcgtcta ttcaacgtca atgctatcca cccgaaattc
     2221 ctcaccctgg gtgatgcgta cctgcatact tgcacaggat gggcattcga aggtttcatc
     2281 atcggggtgg aaggtatgcc cgcaggcgct gcaagccatt tctgcgggga tgcgttcaaa
     2341 gtggagcctt gcaccctgcg ccatggtccc ttcggcgaca atattccagt agaagcttac
     2401 tgagtcatcc acaatggatg cgaattgccc gatcacaagg ttgatatctg tgactcggct
     2461 ggctccagcc tcttttgcat ggcggaggga aatatcgagg atgctttcag taacggcaag
     2521 ttcgtgcatg gcaaccacct taattattgc cagaaaaggg attttcataa gtgaataaag
     2581 tcattccatt aatggacaat tttcctctga tttgttgatc ctccagaggg gagaaaatgc
     2641 caattgagag tggacaggta acgggtccgt attgaacgcg ggcgagggtg atgaaatccc
     2701 aaaagggatg catccacaat ttgtgtagtc attcccatcc gtttgttgtc tctttcggtt
     2761 gaaagttgat agtgtttgat agccccgctg gataaaatta gacttgaaga aaggaactaa
     2821 accatggaaa tcccacgaca ctggcgtctt aaacaacaac gatatggatt ggtaggcgag
     2881 gtctgcccac actgcgatta caagattttt cctccgcgtg atgtgtgccc caactgcgga
     2941 gacgaggcca aggaattatt cacttttagc ggcaagggcc aggtttattc gtacaccact
     3001 gtttatgaag ctccagctgg ctatgatgca aatgcccctt acaccgtggc aatcgtaaaa
     3061 ctagatgaag gtcccatggt gac
//
LOCUS       DAOQJC010000236         2985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_22316_length_2985_cov_11.161775, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000236 DAOQJC010000000
VERSION     DAOQJC010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22316_length_2985_cov_11.161775"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1330)
                     /locus_tag="PLE39_19225"
     CDS             complement(2..1330)
                     /locus_tag="PLE39_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70524.1"
                     /translation="MDDYGKELLRTGIIEAKTGNRDSARRYLDRAIYMSSSFDVLAEA
                     WFWMSEVVDDLAEKRKALENCLAHEMHHARARRSLAILDGKLKADEVVNPDRLPVVTD
                     ETRTSDAQRFMCPKCGGRMSFSPDGQGLVCEYCTRNQTLDSGTASEKDFIVAMATARG
                     HGKPLQEQVFHCKGCGAEFILPPKQISASCAYCNSPHVVSLEKTKDLLAPDGIIPHAF
                     NQKQAIKLLINWVEGNQIKPEKQVELPRGLYLPLWTFDIGGSVEYTYEMEDVNRRNRN
                     RGLQLGEMEPVRITSDYPVMEDDLPLPASKKPSAVFLKMIPTFDMKSIQPYDPRFLVD
                     WPAEIYDVPMADASLDARSRVFAKFKRNLPSMTNHANIVRTSSANLLVESFKLNLLPV
                     WMTELPFNGREHLVLINGQNGVVHSDLAEKVEEEDEKGGLLGFLADLLDD"
     gene            complement(1365..2279)
                     /gene="folP"
                     /locus_tag="PLE39_19230"
     CDS             complement(1365..2279)
                     /gene="folP"
                     /locus_tag="PLE39_19230"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNE70525.1"
                     /translation="MKSSSLQIGSFTFNWGSRTYVMGILNVTPDSFSGDGIIARGDAI
                     HTAVEQARGFLASGADILDVGGESTRPGSAPVNADEEMERVIPIVQALKQNFPDALIS
                     IDTYKAQVAEEAVRAGARIINDVWGLRADDGLAAVAAKYNVPVILMHNRSNPASVEVR
                     EKLGGAYVGSEYQNLLEDVKRELLECVALAKKAQIAESHIVLDPGVGFGKTREHNLEL
                     IDRLGEIRSLGYPVLLGTSRKSFIGFTLDLPADQRVEGTAATVAVGITRGADIIRVHD
                     VKEMARVAKMTDAIVRKPFTINERDKRG"
     gene            complement(2326..2748)
                     /locus_tag="PLE39_19235"
     CDS             complement(2326..2748)
                     /locus_tag="PLE39_19235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70526.1"
                     /translation="MTESKPLNWTPTAEVGYSVIRRGDGGMTLTFTDLSDATMTHWHG
                     FALEHLMGSDRRTRNLYDLRAVKEIPEKAVRMAVDANSDPSARNIRVAVVVADATVAD
                     AIRKVAALALPEAASAMKIFTDINEAEAWLARPLDQIP"
     gene            2875..2948
                     /locus_tag="PLE39_19240"
     tRNA            2875..2948
                     /locus_tag="PLE39_19240"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2909..2911,aa:Val,seq:tac)
BASE COUNT          714 a          872 c          697 g          702 t
ORIGIN      
        1 atcaatcatc caacagatcc gcaagaaagc ccaacagccc acccttttca tcttcctctt
       61 caactttttc tgcgaggtcg ctatgaacca caccgttctg gccgttgatg agcaccagat
      121 gttcgcgccc gttgaacggc agttccgtca tccacaccgg gagcaggttg agtttaaaag
      181 attcgaccaa aagattggca gaagaggtcc gcacgatatt cgcatggttg gtcatgctcg
      241 gaaggttgcg cttgaatttc gcaaacacac ggctgcgtgc atccagtgaa gcgtcagcca
      301 tgggcacatc gtaaatttcg gcaggccaat ccacaagaaa gcgcggatca tacggctgga
      361 tggatttcat atcaaaagtg gggatcatct tcaaaaagac cgccgacggc tttttcgaag
      421 cgggcaatgg caggtcatct tccatcaccg ggtaatcact ggtgatgcgc acaggctcca
      481 tttcaccgag ctgcaacccg cgattcctgt ttcgcctgtt cacatcttcc atttcatagg
      541 tgtattccac cgaaccgcca atatcgaagg tccacagcgg aagatacaac ccgcgcggaa
      601 gttccacctg cttttcaggt ttgatctgat tcccctccac ccaattgatg agcaatttaa
      661 tggcctgctt ctggttgaac gcatgcggaa tgatcccatc gggcgcaagc aggtcttttg
      721 tcttttccag gctcaccaca tgcggcgaat tgcaatacgc acatgaggca gaaatttgtt
      781 tcggcggcaa aataaactcc gcgccgcagc ctttgcaatg aaagacctgt tcctgcaagg
      841 gtttgccgtg tccgcgtgca gtggccatgg caacaataaa atccttttca ctggccgtcc
      901 ctgaatccag ggtctggttg cgtgtgcaat attcacagac caatccctgc ccatcaggtg
      961 aaaaagacat ccgtccgccg cacttggggc acataaaccg ctgcgcgtct gaagtccgtg
     1021 tttcatcggt cacgacaggc aatcggtcgg ggtttacaac ttcgtccgcc ttcagtttcc
     1081 catcaaggat cgccagcgaa cgccgcgcac gtgcatggtg catttcatgc gcaaggcaat
     1141 tctccaatgc cttgcgcttt tcagcgaggt catccacaac ctcgctcatc caaaaccatg
     1201 cctcggccag cacatcaaat gaagacgaca tgtaaatggc ccgatccaaa tagcggcggg
     1261 ctgaatcgcg attgcctgtt ttcgcttcaa tgatccccgt gcgaagaagt tccttgccgt
     1321 aatcgtccat gtttttttaa ccgcagatga acacagataa aaatttatcc gcgcttatcc
     1381 ctttcgttta tggtgaatgg ttttcgcacg atcgcatccg tcatcttcgc cacacgcgcc
     1441 atctctttca catcatgcac acgaataatg tcagcaccgc gcgtgatgcc cactgccacc
     1501 gtcgccgccg taccttccac acgctgatcg gcgggcaggt caagcgtaaa accaataaac
     1561 gatttgcggg atgtccccaa caaaacggga taccccaacg agcggatctc ccccaaccga
     1621 tcgatcaact cgagattatg ctcacgcgtc tttccaaagc cgacgcctgg atccaaaaca
     1681 atatgcgact cagcaatttg ggccttcttc gccaaagcca cacattcaag caattccctt
     1741 tttacatctt caagcagatt ctgatattcc gaacccacat acgccccccc caacttctcg
     1801 cggacttcga cactggctgg gttactgcgg ttgtgcatca aaataacagg cacgttgtat
     1861 ttcgcagcca cagcagccag cccatcatcg gcacgcagcc cccacacgtc attgatgatc
     1921 cgcgccccag cccggaccgc ctcttccgca acttgcgcct tgtaggtgtc aattgaaatc
     1981 aacgcatcag ggaaattctg tttcaacgcc tgcacaatgg ggatcacccg ctccatctct
     2041 tcatctgcat tgactggagc agaaccaggc cgcgtcgact cgccgcctac atccagaata
     2101 tccgcccctg aagcgagaaa cccccgcgcc tgttccacag cggtatggat ggcatctcct
     2161 ctggcaatga tgccatcacc ggaaaacgaa tcgggagtca cattcaagat tcccatcacg
     2221 taggtgcggg aaccccagtt gaatgtaaaa ctcccgattt gcaaggaaga tgatttcata
     2281 aagaaagaaa gcctgaaggt cttcgaaacc catcaggctt tgtgtttacg gtatctggtc
     2341 aagtggacgg gcaagccagg cttccgcctc attaatatcg gtgaatatct tcatcgccga
     2401 agccgcttcc ggaagagcta gtgctgccac tttgcgaatg gcatctgcca cagtcgcatc
     2461 tgcaaccacc accgccacac ggatatttcg cgcagaggga tcgctattgg catccacggc
     2521 catgcgcacc gctttttcag gaatctcctt cacagcgcgc aggtcataca ggttgcgtgt
     2581 gcggcggtct gaacccatca agtgctccag cgcgaatcca tgccaatggg tcatggtggc
     2641 gtctgaaaga tcggtgaagg tcagggtcat gccgccatcg ccgcggcgga tcacggaata
     2701 accgacttca gccgtgggcg tccaattcag tggttttgat tcagtcatgg gtctctcctg
     2761 ttttgattaa tttgattata acgcaggcaa atatcacggg tcggcaaatt tcatgtttgt
     2821 agggaaatcg aaatctttca tttttcgaat cacatgctat aatgcgggtc acccgggcgc
     2881 ttagctcagt tggttagagc acttcgttta caccgaaggg gtcgggggtt cgagtccctc
     2941 agcgcccaca aaataccccg tcaccttggg gtatttttat tttac
//
LOCUS       DAOQJC010000237         2965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_22584_length_2965_cov_7.528866, whole genome shotgun sequence.
ACCESSION   DAOQJC010000237 DAOQJC010000000
VERSION     DAOQJC010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22584_length_2965_cov_7.528866"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PLE39_19245"
     CDS             <1..339
                     /locus_tag="PLE39_19245"
                     /inference="COORDINATES: protein motif:HMM:NF016268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNE70527.1"
                     /translation="CYTRRMAKHKPKPIKFSWDPEKAEKNESKYGVSFDEAMTIFKDP
                     VAMYFDDLEHSQKQKREMVIGRSMPRERLLTCVFERTESGIHIINARISTHRERQDYD
                     DNSEVEYQIQ"
     gene            395..1198
                     /locus_tag="PLE39_19250"
     CDS             395..1198
                     /locus_tag="PLE39_19250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70528.1"
                     /translation="MSRGKKIVIGIIGLLVLLCGAFMLFVNWANSDEGQAAMNEVSTK
                     QAIESATANAQATIQAEADAKAAATATAVMAAINERMQNMTLVHENTFEEGTPLLNEF
                     GEDLEPYYRDGIPELIIPWNGSTILPIGMNLTDFVAEVDCEANGTFCGIAYSYTTDSE
                     GKNHYFASGIGRADTFFFEDLYAKVPSFNWDHKNFAPSTSKEFLHHIRVERFGKVLRL
                     YVNGELMEERILNYPEAVSGDVGFFFGKASNDTGIQTLRMDNFRVWELP"
     gene            1227..1970
                     /locus_tag="PLE39_19255"
     CDS             1227..1970
                     /locus_tag="PLE39_19255"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNE70529.1"
                     /translation="MQLYFIRHGQSINNAKWEEPGYVESPDPYLTEIGQEQAQVLAAY
                     LKANHPITEKEARNNQNQFGYGFTHIYTSLMERAANTAAPTARALGVPFTAWVDIYEE
                     GGIYAREKENLFQGLPGKPRSYFEKNHPALNLPESYNESGWWNRPYESEEERQPRADK
                     VLKELIHRHGDRAGQPEERIVFVSHGGFFVRLLSAMLKLPWRQGAHDMKSWFILNNCS
                     ISRFDFRDGEILINYLNRTDHLPARLITE"
     gene            2013..2273
                     /locus_tag="PLE39_19260"
     CDS             2013..2273
                     /locus_tag="PLE39_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNE70530.1"
                     /translation="MNFLVWIIFGAIAGWVASIIMKRNKQMGALANIIVGVLGAFIGG
                     YIMDFFGAEGVTGFNVPSLLVAILGAVLLLWIGNLLTGRRRR"
     gene            complement(2313..2915)
                     /locus_tag="PLE39_19265"
     CDS             complement(2313..2915)
                     /locus_tag="PLE39_19265"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE70531.1"
                     /translation="MPKKKTYHHGDLKNALIKAGVEILAKDGVSGLSLRKVALKAGVS
                     HAAPYAHFADKQALIAAISTEGFRQLYERVSGVAEKYQSQPEKQLTEAAWAYVQFAMD
                     DRDRFKVMFSGVLEKEKEYSDFVVESQRNFQLVKSIVEANQASGRLRGGDSALVALSA
                     WGIIHGFLMLLLEGQISHAVLEKMSLRELVEFQLEQIKVK"
BASE COUNT          811 a          861 c          646 g          647 t
ORIGIN      
        1 tgctatactc gccgtatggc aaaacacaaa cctaaaccga tcaaattcag ctgggacccc
       61 gaaaaagcgg aaaagaacga atccaaatac ggggtcagtt tcgatgaagc aatgaccatc
      121 ttcaaagacc ctgttgcgat gtatttcgac gacctcgaac actcgcaaaa gcaaaaacgc
      181 gagatggtca ttggcaggtc catgccccgc gaaaggctgt tgacctgtgt gttcgaaaga
      241 accgaaagcg gcattcacat catcaacgcc aggatttcca cacataggga gcggcaggat
      301 tacgacgaca attcggaagt ggaatatcaa atacagtaaa attcaatccc cgtcagatgg
      361 cggggatttt cattcaccaa ggagaaaaag attcatgtca cgcggaaaga aaatcgtcat
      421 cgggatcatt ggcctgctcg ttctgctttg cggagcattc atgctattcg tcaactgggc
      481 gaattcagat gaaggacagg cggcaatgaa cgaggtcagc acaaaacaag ccatcgaaag
      541 cgccacagcc aatgcacagg ccaccatcca ggctgaagca gatgcaaaag ccgcagccac
      601 cgccacggca gtgatggcag ccatcaacga gcggatgcaa aacatgaccc tcgttcatga
      661 aaacactttc gaagaaggca ccccgctctt aaatgaattc ggcgaagacc tcgaacctta
      721 ttatcgtgat ggcattcctg aactcatcat cccgtggaac ggctcaacca tcctgcccat
      781 cggaatgaac ctgaccgatt ttgttgccga agtggattgc gaagcaaacg gcacgttctg
      841 cggcatcgcc tacagctaca caaccgactc tgaaggtaaa aatcactact tcgcctcagg
      901 catcggaaga gccgacactt ttttctttga agatttatat gccaaagtgc catccttcaa
      961 ttgggatcat aaaaacttcg ccccttccac ctctaaagaa ttcctacacc acatccgcgt
     1021 ggaacgcttt ggaaaagtac tccgcctgta tgtcaacggt gagttgatgg aagaacgcat
     1081 cctcaactac cccgaagctg tttctggcga tgtgggcttt ttcttcggga aggcctccaa
     1141 cgacacaggc attcagaccc tccgaatgga taacttccgc gtctgggaac tgccgtaaac
     1201 caaacttcaa acctacagag accatcatgc aactttactt catccgtcat ggacaatcca
     1261 tcaacaacgc aaaatgggaa gagcccggtt acgtggaaag cccagaccca tatctgacag
     1321 agatcgggca ggaacaggcg caagtcctcg ccgcatattt aaaagcaaac caccccatca
     1381 ccgaaaaaga ggcacggaat aaccaaaacc agttcgggta tggtttcacc catatctaca
     1441 ccagcctgat ggaacgggct gccaacaccg ccgcacccac cgcccgcgca ttgggcgtcc
     1501 cattcacagc ctgggtggat atttacgaag aaggcggaat ctatgcccgt gaaaaggaaa
     1561 atctctttca aggcctgcct ggcaagccgc gttcgtattt cgagaaaaac catcctgcat
     1621 tgaatctgcc tgaaagctac aacgaatcgg gctggtggaa tcgtccctac gaatcggaag
     1681 aagagcgcca gccgcgagcc gacaaagtcc tcaaagagct gatccatcgt catggcgaca
     1741 gggcaggcca acccgaagaa aggatcgtct tcgtcagcca cggcggattt tttgtccgcc
     1801 tgctttctgc catgctaaaa cttccctggc ggcagggcgc acatgatatg aagtcgtggt
     1861 tcattctcaa caattgctcc atcagccgct ttgattttcg agacggcgaa attctcatca
     1921 attatcttaa ccgcaccgat cacctccctg cacgcctcat taccgaatga taaaataaaa
     1981 aaacgaaacc aacttatcta ttggaggcaa aaatgaactt tctcgtttgg atcatcttcg
     2041 gcgcgatcgc aggctgggtt gccagcatca tcatgaagcg caacaaacaa atgggagcac
     2101 tcgccaatat catcgtcggt gttctcggcg ctttcatcgg cggttacatc atggatttct
     2161 tcggcgctga aggcgtcact ggcttcaacg tcccaagcct gcttgtcgcc atccttggcg
     2221 cagtgctgct gctctggatc ggcaacctgc tcacgggtcg ccgccgcaga taatttcaaa
     2281 aagagccgtg caacgaattg cacggctctt ttttatttca ctttgatctg ctccaactga
     2341 aactccacca gttcacgcaa gctcatcttc tccaaaaccg cgtgcgagat ctgcccctca
     2401 agcagcaaca tcaaaaaccc gtggatgatt ccccacgccg aaagagccac caacgccgaa
     2461 tcaccgcctc tcaacctacc tgacgcctga ttcgcctcta caatgctttt caccagctga
     2521 aaatttctct gcgactccac cacaaaatcg gaatattcct tttccttctc cagcaccccc
     2581 gaaaacatga ccttgaatcg gtcacggtca tccatcgcaa actggacata cgcccaagcc
     2641 gcctcggtca attgcttttc gggctggctt tgatatttct ccgccacccc actcacccgc
     2701 tcgtatagtt gacgaaaccc ttcagtggaa attgccgcga tcaacgcctg cttatccgca
     2761 aaatgagcat acggcgcggc atgactcacc cccgccttca acgccacctt ccgcagggac
     2821 agtccgctca ccccatcctt cgcaaggatc tccaccccag ctttgataag cgcattcttc
     2881 aaatcaccgt gatgataggt tttcttcttt ggcattctcg tctctcaatc ttgacactgt
     2941 aaagattgta gaggataatt tattt
//
LOCUS       DAOQJC010000238         2556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_29206_length_2556_cov_8.747701, whole genome shotgun sequence.
ACCESSION   DAOQJC010000238 DAOQJC010000000
VERSION     DAOQJC010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29206_length_2556_cov_8.747701"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PLE39_19270"
     CDS             <1..402
                     /locus_tag="PLE39_19270"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNE70532.1"
                     /translation="TRVPKLTLVIGGSFGAGNYAMAGRAYGSRFLWMWPNARISVMGG
                     EQAANVLLTIKQDQLVREGKPALSTEEADEFKRPLLEKYETEGSPYHSSARLWDDGVI
                     APDETRQVLGLALSIVLNSPKEEGGFGVFRM"
     gene            432..908
                     /locus_tag="PLE39_19275"
     CDS             432..908
                     /locus_tag="PLE39_19275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNE70533.1"
                     /translation="MRYVEWQAGVPDEIKGDSLWKLEVYRLGLFAADIAWQDALVLNK
                     NPLTRGLADQLYRAVCSISANIAEGYSRSTGKDRARFLEYSLGSARESRDWYFKSRHT
                     LKEEVVKHRIDLLTQIIKMLSVLTPNQRQKGIREEQAEYVILQSSDFNIDLETPLP"
     gene            944..1429
                     /locus_tag="PLE39_19280"
     CDS             944..1429
                     /locus_tag="PLE39_19280"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HNE70534.1"
                     /translation="MTFSNSIDTPHHFLAQLAGNWRGTSKLWMEPGKLADESQIVGTI
                     QLLLEGRFALFLYQSSIEGEAQHGMFTFGYNTSLEQFETSWVDSFHTNTGIMFCTGRE
                     LGGGFSVTGSYPDPSGGPDWNWRTEVELNGDQLTITAYNITPEGEEAKATEMALTRLK
                     K"
     gene            1552..2541
                     /locus_tag="PLE39_19285"
     CDS             1552..2541
                     /locus_tag="PLE39_19285"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE70535.1"
                     /translation="MKILIIGGTRFLGRHLVESALARGHEVTLFNRGKSNPDLFKQVQ
                     TIRGDREKDLDQISGKYDAVIDTCGYFPRIVRMSAEALKGKARSYVFISSISVYAGFS
                     KIGINESDPVGKIEDETIEEITGESYGPLKALCENAVQDVFGEQALIVRPGLIVGPHD
                     PTDRFTYWPVRVARGGDVLTPEKPDVPIQIIDVRDLSDFVIELLQQNEFGVFNATGPA
                     HELTLGAMLNACKQVSGSGANFKWASVEFLNQNQVAPWSDMPVWVPNVPEDAGFSRVD
                     ISKAMRSGLKFMPLEKTIHDTLEWEKSRPSDHEWRAGLKPEREKELLRLLDVQ"
BASE COUNT          660 a          633 c          647 g          616 t
ORIGIN      
        1 acccgcgtcc ccaaattgac tctcgtcatc ggcggctcct ttggcgcagg caactacgcc
       61 atggcaggcc gtgcctacgg ctcccgcttc ctgtggatgt ggcccaacgc ccgtatctcc
      121 gtcatgggcg gggagcaagc tgcgaatgtg ctcttaacca tcaaacaaga tcaactcgtc
      181 agagaaggca aaccagctct cagcacggaa gaagcggacg agttcaagcg tccactgttg
      241 gaaaaatacg aaaccgaagg cagtccctat cattcctccg cccgcctttg ggacgatggc
      301 gtcattgctc ccgatgaaac gagacaagtg ttggggttgg cgttatccat cgttttgaat
      361 tcaccgaagg aagagggtgg gtttggggtg tttaggatgt agaaagtgga aagtggtgag
      421 tggaggatgg catgaggtat gttgaatggc aagcgggtgt tcctgatgaa atcaaaggtg
      481 attcgctttg gaaattagaa gtctatcgct taggtttatt tgccgccgac attgcatggc
      541 aggatgcgct tgttttgaat aaaaatccat taacccgtgg acttgccgac caactctatc
      601 gagcggtgtg ctctatatct gcaaacatag ctgaagggta ctcgcgttct acaggaaaag
      661 accgtgctcg gtttctcgaa tactcacttg gttctgcaag agaatctcgt gattggtatt
      721 tcaaatcacg ccatacgctg aaagaagaag ttgtcaaaca tcgaattgac ttgttgactc
      781 aaatcatcaa aatgttaagt gtccttacac ccaatcaacg ccaaaaaggt atccgcgaag
      841 aacaagctga atacgttatt ttacaatcat cagatttcaa tattgattta gagactccac
      901 ttccataatc cactcaccac tttccactca ctagaggtac tccatgacct tctcaaactc
      961 aatcgacacc cctcatcact tcctcgccca gcttgctggt aactggcgcg gcacatccaa
     1021 actttggatg gagccaggca aacttgcaga tgaatctcag atcgttggaa ccatccaact
     1081 cctgctggaa ggacgctttg cccttttcct gtatcaatcg tccattgaag gtgaagcaca
     1141 gcatggcatg tttacctttg gctataacac cagcctcgaa caattcgaaa cctcatgggt
     1201 ggattcgttc cacaccaaca cgggcatcat gttttgcaca ggcagggaat tgggcggcgg
     1261 tttctctgtc actggcagtt atcccgaccc cagcggaggc ccggattgga actggcgcac
     1321 cgaagtggaa ttgaacggcg atcaactgac catcaccgcc tacaacatca cccctgaagg
     1381 ggaagaagcc aaggccaccg aaatggcgct gacaagattg aagaaataaa agtgtcatgt
     1441 cgttgcgagg agcgctcttg ttttttgcga cgaagcaacc tccaattacg aacgagattg
     1501 cttcgtcggg acgaacaccc tcctcgcaac gacatatcaa atggagacaa aatgaaaatc
     1561 ctcatcattg gcggcacacg ttttttaggc aggcatctcg ttgagtcggc gctggctcgc
     1621 ggacatgaag tgaccttgtt caatcgcggc aaatccaacc ctgatctgtt taagcaagtc
     1681 caaaccatca ggggtgatcg tgaaaaagat ctggatcaga tttcagggaa gtatgatgcg
     1741 gtcattgata cctgcggata ttttccgcgc atcgtccgca tgtccgccga agcgttgaag
     1801 ggcaaggccc ggtcttatgt ttttatctca agcatttcgg tgtacgcagg tttcagcaaa
     1861 attggaatca acgaaagcga ccccgttgga aagattgaag atgaaaccat cgaagagatc
     1921 acgggcgagt cgtatggccc gttgaaggca ttgtgtgaaa acgccgtgca ggatgtcttt
     1981 ggcgaacagg cattgatcgt ccgccctgga ctcattgttg gcccgcatga ccccactgac
     2041 cgattcacct attggcctgt gcgcgtggct cgcggcggcg atgtgctcac gcccgaaaag
     2101 cccgatgtgc caattcaaat tattgatgtc cgtgacctgt ccgattttgt cattgagctt
     2161 cttcaacaaa atgagtttgg cgttttcaat gcaaccggcc cagcgcatga actgactttg
     2221 ggtgcaatgt tgaacgcctg taaacaggtc agcggcagcg gcgcgaattt caaatgggca
     2281 tcggttgagt tcctaaatca gaaccaggtt gcgccgtgga gcgatatgcc tgtttgggtt
     2341 cccaatgtgc ctgaagatgc aggcttctcc cgtgtggata tttcaaaagc gatgcgatct
     2401 ggcttgaaat ttatgccgct cgaaaaaacg attcatgaca cgctcgaatg ggaaaagtcg
     2461 cgtccgtcag atcatgaatg gcgcgctggc ttgaaacccg aacgtgaaaa ggaattactg
     2521 aggctgctcg atgttcaata aaattctcat cgccaa
//
LOCUS       DAOQJC010000239         2335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_34370_length_2335_cov_11.463596, whole genome shotgun
            sequence.
ACCESSION   DAOQJC010000239 DAOQJC010000000
VERSION     DAOQJC010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34370_length_2335_cov_11.463596"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..716)
                     /locus_tag="PLE39_19290"
     CDS             complement(75..716)
                     /locus_tag="PLE39_19290"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNE70536.1"
                     /translation="MYIAIEGVIGVGKTTLARLLQTTFEAEVLLEVFEENPFLSDFYA
                     DRARYAFQTQIFFLLSRYHQQNNNVPKILADGKNLLADYTFAKDALFAGINMQGDELD
                     MYHKVHEALGEKIPKPDLIVYLQATTDTLMSRIAFRDRPYERQMERAYIDELNHAYEN
                     FFSKPFDHVPVLKIDSNELDIIRNAEHLKLIENRIRSTLGLIPFQQNLPLPEK"
     gene            816..1859
                     /locus_tag="PLE39_19295"
     CDS             816..1859
                     /locus_tag="PLE39_19295"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE70537.1"
                     /translation="MSKICIVPKVDGIGGMASFRLKFEQGLRARGVDVTHDLSEDSDA
                     ALVIAGTRNLIPLWRARQRGRRVVQRLDGINWVHRKMNTGLKHYLRAEYGNFVLSFIR
                     RFIATDIIYQSEFSRDWWERKYGKTRVPFAVIHNGIDLNAYSVKRDNIPLYPPFRLLL
                     VEGNLGGGYDMGLDNAIALTETLIEKHNLPVELMAVGKISEEHRQRVEAKTRAPIQWM
                     GSVPRERIPEIMRSAHLFFSADLHPACPNSVIEALACGLPVVGFDTGAVKELVIGDAG
                     RVVPYGSNSWNIEPPDISALAVVAKEILQNQPHFSQAARTHAESALGLDKMVDAYLKV
                     LLTTKGHEGTQRF"
     gene            1923..>2335
                     /locus_tag="PLE39_19300"
     CDS             1923..>2335
                     /locus_tag="PLE39_19300"
                     /inference="COORDINATES: protein motif:HMM:NF019932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNE70538.1"
                     /translation="MDNEIHEFLLKHLHGIMNNDIASYHETTAEDLTLYEWWVTPHRL
                     DGLPFHEFMMNSNASRGSVFGAEDQGQKAQSRFDLANLHIQRYGDTAIASYTLLISTA
                     LPEGVKVAAHNESRVMVKMDGKWKVVHVHKSPAYSA"
BASE COUNT          576 a          581 c          629 g          549 t
ORIGIN      
        1 ctcttttgcg attcgaagaa gtgattcacg tgttacgcgc atagggattt cctttgtatc
       61 tggggcgaag agaactattt ttcggggaga ggcaaattct gttgaaacgg aatcagccca
      121 agggtcgatc ggatgcggtt ctctatcagc ttgagatgtt cggcgttgcg gataatgtcc
      181 agttcgttcg agtcaatttt caaaacgggg acatggtcaa acggtttcga aaagaaattt
      241 tcatacgcgt gattgagttc gtcaatgtag gcgcgttcca tttgtctttc gtacggcctg
      301 tcgcggaagg caattcgtga catgagcgtg tcggtggtgg cctgcaaata cacgatcaga
      361 tcgggcttgg gaattttctc gccaagcgcc tcgtgaacct tgtgatacat atcgagctcg
      421 tcgccctgca tgttgatgcc cgcaaacaac gcatccttcg caaaggtgta atcggcgagc
      481 aggttttttc catcggccaa aatcttcggg acgttgttat tttgttgatg atagcggctg
      541 agtaaaaaga agatctgggt ttgaaatgcg taccgtgcgc ggtcagcata aaaatcagaa
      601 aggaaggggt tctcctcgaa aacctcaagc agaacctcgg cttcgaatgt agtttgcaga
      661 aggcgggcaa gtgtggtctt gccaacgccg atcacccctt caatggcaat gtacatgaaa
      721 aaagctccct gagtggattg ccgcaaggat accataagtt aaggatgaag gagaaaggat
      781 gagggctgaa aaatggaaga ttgtataatg gatgaatgtc taaaatttgc atcgttccaa
      841 aagtggatgg gatcggcggc atggcatcct tccgcttgaa atttgagcag ggactgcgcg
      901 cgcgcggcgt ggatgtgaca cacgatcttt ctgaggattc agacgctgcg ctcgttattg
      961 ccgggacccg aaacctgatc ccgttatgga gagcccgtca gcgcggacgg cgcgttgtgc
     1021 aacgcctcga cggaatcaac tgggttcacc gcaaaatgaa cacgggactc aaacattacc
     1081 tgcgtgccga gtatggcaac tttgtgttgt cgttcatccg ccgttttatt gcgaccgata
     1141 ttatttatca aagcgaattt tcgcgcgatt ggtgggaacg taaatacggc aagacacggg
     1201 tgccgttcgc tgtgattcac aatgggattg atttgaatgc gtattctgta aagcgggata
     1261 atatcccgct ctacccgccc ttccgccttt tgctcgtcga aggcaatctc ggcggcggct
     1321 acgacatggg tctggacaat gccatcgcac tcaccgaaac cctgatcgaa aaacacaacc
     1381 tgcccgtgga attgatggct gtcgggaaaa tttccgagga acaccgtcag cgggtggaag
     1441 caaaaacccg tgcgccgatt caatggatgg gcagcgtacc gcgtgagcgc atccctgaga
     1501 tcatgcgctc ggcgcatctg ttcttctccg ctgacttgca ccccgcctgc ccgaattcgg
     1561 tcatcgaagc actggcctgc ggattgcccg tcgtgggttt cgatacaggc gcggtcaagg
     1621 aattggtcat cggcgatgct ggcagggttg tgccgtacgg aagcaactcc tggaatatcg
     1681 aaccgcccga tatttccgcg ctcgccgttg tcgccaaaga gattttgcaa aaccagccgc
     1741 atttcagcca agctgcgcgg actcacgccg aatccgcgct ggggctggat aaaatggtgg
     1801 atgcatattt gaaagtccta ttaaccacaa aggggcacga aggtacacaa aggttttaaa
     1861 agggtttccc ttcgtgcccc tttgtgacac ttcgtggttg aatcttttac agaggtcaaa
     1921 tcatggataa cgaaattcac gaattcctcc tcaaacacct gcatggaatt atgaacaacg
     1981 acatcgcgtc gtatcatgaa accacagccg aagacctcac gctttatgag tggtgggtca
     2041 cgccgcaccg tctggacggt cttcccttcc acgaattcat gatgaactcc aatgcctcac
     2101 gcggatccgt cttcggcgcg gaagatcagg gacaaaaagc gcaaagtcgc tttgacctcg
     2161 ccaacttgca cattcaacgc tacggcgata ccgccattgc cagctacaca ttgctcatca
     2221 gcaccgcgct cccagaaggg gtcaaagtgg ctgcacacaa cgaaagccgc gtaatggtta
     2281 agatggacgg aaagtggaaa gtagtccacg tgcataaatc ccccgcctac tccgc
//
LOCUS       DAOQJC010000240         1805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0610
            NODE_56234_length_1805_cov_7.663429, whole genome shotgun sequence.
ACCESSION   DAOQJC010000240 DAOQJC010000000
VERSION     DAOQJC010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810242
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,862
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56234_length_1805_cov_7.663429"
                     /isolate="AMR_MDS_0610"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PLE39_19305"
     CDS             <1..627
                     /locus_tag="PLE39_19305"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE70539.1"
                     /translation="MERVVMVDLDGEVVEACKIHLPEMHQGAFDDPRVELVIQDAVAY
                     LDSSPSEWDVIISDLTDPIESGPSFKLFTKEYFSKAQKALRPGGYFLLQAGPVSPPSM
                     DMHIRLVSTLKAVFKYVHSYHIHVPMLGHPWGFILASDSPINTRPEPAEVDQLLTEKT
                     NGIFKMFDGITLLGLMQSPRHIRDAIAKETTVYTLEHPPVFSTQELPK"
     gene            723..1550
                     /gene="speE"
                     /locus_tag="PLE39_19310"
     CDS             723..1550
                     /gene="speE"
                     /locus_tag="PLE39_19310"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNE70540.1"
                     /translation="MSIWFTEELHPYYRKGIRIKNILADEQTPFQHVQVVETEFFGKA
                     MILDGIIQLTERDNMGYHEMIVHVPMLAVGQPKRVLIVGGGDGGSLQQVLRYPSVEEA
                     IVCELDQRVVDLSREHFASFGDPWADPRARLLVRDAFGYLEENPSQFDVIISDTTDPI
                     GMAERLFSEEFYKLMVRALTPNGAAATQCEQPFFDTELIKQIHHSAKTLTKNPAYYYA
                     NIPTYPGGGIGFMYVSNTPWENGLKTPFPPGKNQYINPEIHKAAFALPEFFRRELMG"
BASE COUNT          427 a          487 c          434 g          457 t
ORIGIN      
        1 atggaacggg ttgtgatggt ggacctcgac ggggaagtgg tggaagcctg caagattcat
       61 ctccctgaaa tgcaccaggg cgcgttcgac gacccgcgtg tcgagttggt cattcaagat
      121 gcagttgcct atctcgattc ttctccatct gaatgggatg tcatcatctc agacctgacc
      181 gaccccatcg aatctgggcc gtctttcaag ttattcacca aggaatattt ctccaaggcg
      241 caaaaagcac tgcgcccggg cgggtatttt ctccttcagg cagggccagt ttccccgcct
      301 tcgatggata tgcacattcg cctggtttcc actcttaagg cagtgttcaa gtatgtccat
      361 tcgtatcaca tccatgtgcc catgctgggg cacccgtggg gctttatcct tgcaagcgat
      421 tccccgatca acacccgccc cgaacctgcg gaagtggatc agttgctgac agagaagacc
      481 aatggcattt tcaagatgtt tgatggcatt accctgttgg gtttgatgca atcgcccagg
      541 cacatccgcg atgcgatcgc aaaagagacc acggtgtaca cgcttgagca tccgcctgtt
      601 ttcagcacac aggaactgcc caagtagtaa aatatccata gtcttctaca ggtcaccaga
      661 ccgccgacca tgggccgtag atttcggtcc ttcgtcggcg gtttttatta aaaggaattt
      721 ccatgagcat ttggttcaca gaagagcttc acccttacta tcgcaaaggc atccgcatca
      781 agaacatact ggccgatgag cagaccccgt ttcaacatgt tcaggtggtt gaaacagagt
      841 ttttcggcaa agccatgatt ctggatggca tcattcaact taccgagcgc gataacatgg
      901 gctatcatga aatgatcgtc catgttccca tgctggcggt tggtcagcca aaacgtgtgc
      961 tcattgtcgg cggcggggat ggcggctcgc ttcaacaggt cttgcgatat ccctccgtcg
     1021 aagaggccat tgtctgtgaa ttggatcagc gtgtggtgga tctctcccga gagcacttcg
     1081 cttcttttgg cgacccttgg gctgatcctc gtgccaggct gctcgtacgg gacgcatttg
     1141 gttacctcga agaaaacccc tcccaattcg atgtcatcat ctcagatacc accgacccca
     1201 ttggcatggc cgaacggctt ttctctgagg aattttacaa actcatggtc cgcgctctga
     1261 cgcccaacgg cgctgccgcc actcaatgcg aacagccgtt tttcgacacg gaattgatca
     1321 agcagatcca tcactccgcc aaaacgctga cgaagaaccc cgcttattac tacgctaata
     1381 tccccaccta tcctggcggc ggcattggct ttatgtatgt ctcaaatacc ccgtgggaaa
     1441 acggcctgaa aaccccgttc ccgcctggga aaaaccaata tatcaaccct gaaatccaca
     1501 aagcggcttt tgccctgccc gagtttttca ggcgggaatt gatgggctga ttctcaaaaa
     1561 cccttgagct tttcaagggt tttataatac ccacggtcac aaattgcgct ttcccgcctt
     1621 tgaaaaggca taatttgtga ccgagtgcgg tatattttca aaggttataa tctgtacaga
     1681 ggcatcttat gagcaaactc attgtttcct actcacgaaa agattccgtg gttgcccgca
     1741 aattgatcga tacgttcaaa aagaacgaat tcgatgtctg ggtggattgg gaggatattc
     1801 cgcct
//
